Id |
Subject |
Object |
Predicate |
Lexical cue |
T2624 |
0-2 |
RB |
denotes |
At |
T2626 |
3-8 |
RBS |
denotes |
least |
T2625 |
9-12 |
CD |
denotes |
419 |
T2628 |
13-18 |
NN |
denotes |
mouse |
T2629 |
19-28 |
JJ |
denotes |
olfactory |
T2630 |
29-37 |
NN |
denotes |
receptor |
T2627 |
38-43 |
NNS |
denotes |
genes |
T2632 |
44-47 |
VBP |
denotes |
are |
T2631 |
48-57 |
VBN |
denotes |
expressed |
T2633 |
58-60 |
IN |
denotes |
in |
T2634 |
61-64 |
DT |
denotes |
the |
T2636 |
65-74 |
JJ |
denotes |
olfactory |
T2635 |
75-85 |
NN |
denotes |
epithelium |
T2637 |
85-242 |
sentence |
denotes |
We have isolated 1,264 olfactory receptor cDNA clones, which together confirm the olfactory epithelial expression of 419 annotated olfactory receptor genes. |
T2638 |
86-88 |
PRP |
denotes |
We |
T2640 |
89-93 |
VBP |
denotes |
have |
T2639 |
94-102 |
VBN |
denotes |
isolated |
T2641 |
103-108 |
CD |
denotes |
1,264 |
T2643 |
109-118 |
JJ |
denotes |
olfactory |
T2644 |
119-127 |
NN |
denotes |
receptor |
T2645 |
128-132 |
NN |
denotes |
cDNA |
T2642 |
133-139 |
NNS |
denotes |
clones |
T2646 |
139-141 |
, |
denotes |
, |
T2647 |
141-146 |
WDT |
denotes |
which |
T2649 |
147-155 |
RB |
denotes |
together |
T2648 |
156-163 |
VBP |
denotes |
confirm |
T2650 |
164-167 |
DT |
denotes |
the |
T2652 |
168-177 |
JJ |
denotes |
olfactory |
T2653 |
178-188 |
JJ |
denotes |
epithelial |
T2651 |
189-199 |
NN |
denotes |
expression |
T2654 |
200-202 |
IN |
denotes |
of |
T2655 |
203-206 |
CD |
denotes |
419 |
T2657 |
207-216 |
VBN |
denotes |
annotated |
T2658 |
217-226 |
JJ |
denotes |
olfactory |
T2659 |
227-235 |
NN |
denotes |
receptor |
T2656 |
236-241 |
NNS |
denotes |
genes |
T2660 |
241-242 |
. |
denotes |
. |
T2661 |
242-500 |
sentence |
denotes |
We used low-stringency hybridization with degenerate olfactory receptor DNA probes to screen around 4,100,000 plaque-forming units (pfu) of an adult mouse olfactory epithelium cDNA library and around 640,000 pfu of an embryonic olfactory epithelium library. |
T2662 |
243-245 |
PRP |
denotes |
We |
T2663 |
246-250 |
VBD |
denotes |
used |
T2664 |
251-254 |
JJ |
denotes |
low |
T2666 |
254-255 |
HYPH |
denotes |
- |
T2665 |
255-265 |
NN |
denotes |
stringency |
T2667 |
266-279 |
NN |
denotes |
hybridization |
T2668 |
280-284 |
IN |
denotes |
with |
T2669 |
285-295 |
JJ |
denotes |
degenerate |
T2671 |
296-305 |
JJ |
denotes |
olfactory |
T2672 |
306-314 |
NN |
denotes |
receptor |
T2673 |
315-318 |
NN |
denotes |
DNA |
T2670 |
319-325 |
NNS |
denotes |
probes |
T2674 |
326-328 |
TO |
denotes |
to |
T2675 |
329-335 |
VB |
denotes |
screen |
T2676 |
336-342 |
RB |
denotes |
around |
T2677 |
343-352 |
CD |
denotes |
4,100,000 |
T2679 |
353-359 |
NN |
denotes |
plaque |
T2681 |
359-360 |
HYPH |
denotes |
- |
T2680 |
360-367 |
VBG |
denotes |
forming |
T2678 |
368-373 |
NNS |
denotes |
units |
T2682 |
374-375 |
-LRB- |
denotes |
( |
T2683 |
375-378 |
NNS |
denotes |
pfu |
T2684 |
378-379 |
-RRB- |
denotes |
) |
T2685 |
380-382 |
IN |
denotes |
of |
T2686 |
383-385 |
DT |
denotes |
an |
T2688 |
386-391 |
JJ |
denotes |
adult |
T2689 |
392-397 |
NN |
denotes |
mouse |
T2691 |
398-407 |
JJ |
denotes |
olfactory |
T2692 |
408-418 |
NN |
denotes |
epithelium |
T2690 |
419-423 |
NN |
denotes |
cDNA |
T2687 |
424-431 |
NN |
denotes |
library |
T2693 |
432-435 |
CC |
denotes |
and |
T2694 |
436-442 |
RB |
denotes |
around |
T2695 |
443-450 |
CD |
denotes |
640,000 |
T2696 |
451-454 |
NNS |
denotes |
pfu |
T2697 |
455-457 |
IN |
denotes |
of |
T2698 |
458-460 |
DT |
denotes |
an |
T2700 |
461-470 |
JJ |
denotes |
embryonic |
T2702 |
471-480 |
JJ |
denotes |
olfactory |
T2701 |
481-491 |
NN |
denotes |
epithelium |
T2699 |
492-499 |
NN |
denotes |
library |
T2703 |
499-500 |
. |
denotes |
. |
T2704 |
500-616 |
sentence |
denotes |
We obtained sequences from 1,715 hybridization-positive cDNAs following secondary screens to isolate single clones. |
T2705 |
501-503 |
PRP |
denotes |
We |
T2706 |
504-512 |
VBD |
denotes |
obtained |
T2707 |
513-522 |
NNS |
denotes |
sequences |
T2708 |
523-527 |
IN |
denotes |
from |
T2709 |
528-533 |
CD |
denotes |
1,715 |
T2711 |
534-547 |
NN |
denotes |
hybridization |
T2713 |
547-548 |
HYPH |
denotes |
- |
T2712 |
548-556 |
JJ |
denotes |
positive |
T2710 |
557-562 |
NNS |
denotes |
cDNAs |
T2714 |
563-572 |
VBG |
denotes |
following |
T2715 |
573-582 |
JJ |
denotes |
secondary |
T2716 |
583-590 |
NNS |
denotes |
screens |
T2717 |
591-593 |
TO |
denotes |
to |
T2718 |
594-601 |
VB |
denotes |
isolate |
T2719 |
602-608 |
JJ |
denotes |
single |
T2720 |
609-615 |
NNS |
denotes |
clones |
T2721 |
615-616 |
. |
denotes |
. |
T2722 |
616-688 |
sentence |
denotes |
Of these clones, 1,264 yielded olfactory receptor-containing sequences. |
T2723 |
617-619 |
IN |
denotes |
Of |
T2725 |
620-625 |
DT |
denotes |
these |
T2726 |
626-632 |
NNS |
denotes |
clones |
T2727 |
632-634 |
, |
denotes |
, |
T2728 |
634-639 |
CD |
denotes |
1,264 |
T2724 |
640-647 |
VBD |
denotes |
yielded |
T2729 |
648-657 |
JJ |
denotes |
olfactory |
T2730 |
658-666 |
NN |
denotes |
receptor |
T2732 |
666-667 |
HYPH |
denotes |
- |
T2731 |
667-677 |
VBG |
denotes |
containing |
T2733 |
678-687 |
NNS |
denotes |
sequences |
T2734 |
687-688 |
. |
denotes |
. |
T2735 |
688-802 |
sentence |
denotes |
The 26% false-positive rate is a consequence of using low-stringency hybridization to obtain maximal sensitivity. |
T2736 |
689-692 |
DT |
denotes |
The |
T2738 |
693-695 |
CD |
denotes |
26 |
T2739 |
695-696 |
NN |
denotes |
% |
T2740 |
697-702 |
JJ |
denotes |
false |
T2742 |
702-703 |
HYPH |
denotes |
- |
T2741 |
703-711 |
JJ |
denotes |
positive |
T2737 |
712-716 |
NN |
denotes |
rate |
T2743 |
717-719 |
VBZ |
denotes |
is |
T2744 |
720-721 |
DT |
denotes |
a |
T2745 |
722-733 |
NN |
denotes |
consequence |
T2746 |
734-736 |
IN |
denotes |
of |
T2747 |
737-742 |
VBG |
denotes |
using |
T2748 |
743-746 |
JJ |
denotes |
low |
T2750 |
746-747 |
HYPH |
denotes |
- |
T2749 |
747-757 |
NN |
denotes |
stringency |
T2751 |
758-771 |
NN |
denotes |
hybridization |
T2752 |
772-774 |
TO |
denotes |
to |
T2753 |
775-781 |
VB |
denotes |
obtain |
T2754 |
782-789 |
JJ |
denotes |
maximal |
T2755 |
790-801 |
NN |
denotes |
sensitivity |
T2756 |
801-802 |
. |
denotes |
. |
T2757 |
802-1110 |
sentence |
denotes |
Continuing the screen would have resulted in cDNAs from additional olfactory receptors, but we reached a point of limiting returns: our final batch of 45 olfactory receptor-positive sequences represented 33 different olfactory receptors, of which only four had not been encountered previously in our screen. |
T2758 |
803-813 |
VBG |
denotes |
Continuing |
T2760 |
814-817 |
DT |
denotes |
the |
T2761 |
818-824 |
NN |
denotes |
screen |
T2762 |
825-830 |
MD |
denotes |
would |
T2763 |
831-835 |
VB |
denotes |
have |
T2759 |
836-844 |
VBN |
denotes |
resulted |
T2765 |
845-847 |
IN |
denotes |
in |
T2766 |
848-853 |
NNS |
denotes |
cDNAs |
T2767 |
854-858 |
IN |
denotes |
from |
T2768 |
859-869 |
JJ |
denotes |
additional |
T2770 |
870-879 |
JJ |
denotes |
olfactory |
T2769 |
880-889 |
NNS |
denotes |
receptors |
T2771 |
889-891 |
, |
denotes |
, |
T2772 |
891-894 |
CC |
denotes |
but |
T2773 |
895-897 |
PRP |
denotes |
we |
T2774 |
898-905 |
VBD |
denotes |
reached |
T2775 |
906-907 |
DT |
denotes |
a |
T2776 |
908-913 |
NN |
denotes |
point |
T2777 |
914-916 |
IN |
denotes |
of |
T2778 |
917-925 |
VBG |
denotes |
limiting |
T2779 |
926-933 |
NNS |
denotes |
returns |
T2780 |
933-935 |
: |
denotes |
: |
T2781 |
935-938 |
PRP$ |
denotes |
our |
T2783 |
939-944 |
JJ |
denotes |
final |
T2782 |
945-950 |
NN |
denotes |
batch |
T2784 |
951-953 |
IN |
denotes |
of |
T2785 |
954-956 |
CD |
denotes |
45 |
T2787 |
957-966 |
JJ |
denotes |
olfactory |
T2788 |
967-975 |
NN |
denotes |
receptor |
T2790 |
975-976 |
HYPH |
denotes |
- |
T2789 |
976-984 |
JJ |
denotes |
positive |
T2786 |
985-994 |
NNS |
denotes |
sequences |
T2764 |
995-1006 |
VBD |
denotes |
represented |
T2791 |
1007-1009 |
CD |
denotes |
33 |
T2793 |
1010-1019 |
JJ |
denotes |
different |
T2794 |
1020-1029 |
JJ |
denotes |
olfactory |
T2792 |
1030-1039 |
NNS |
denotes |
receptors |
T2795 |
1039-1041 |
, |
denotes |
, |
T2796 |
1041-1043 |
IN |
denotes |
of |
T2798 |
1044-1049 |
WDT |
denotes |
which |
T2799 |
1050-1054 |
RB |
denotes |
only |
T2800 |
1055-1059 |
CD |
denotes |
four |
T2801 |
1060-1063 |
VBD |
denotes |
had |
T2802 |
1064-1067 |
RB |
denotes |
not |
T2803 |
1068-1072 |
VBN |
denotes |
been |
T2797 |
1073-1084 |
VBN |
denotes |
encountered |
T2804 |
1085-1095 |
RB |
denotes |
previously |
T2805 |
1096-1098 |
IN |
denotes |
in |
T2806 |
1099-1102 |
PRP$ |
denotes |
our |
T2807 |
1103-1109 |
NN |
denotes |
screen |
T2808 |
1109-1110 |
. |
denotes |
. |
T2809 |
1110-1174 |
sentence |
denotes |
Sequence analysis shows that the libraries are of high quality. |
T2810 |
1111-1119 |
NN |
denotes |
Sequence |
T2811 |
1120-1128 |
NN |
denotes |
analysis |
T2812 |
1129-1134 |
VBZ |
denotes |
shows |
T2813 |
1135-1139 |
IN |
denotes |
that |
T2815 |
1140-1143 |
DT |
denotes |
the |
T2816 |
1144-1153 |
NNS |
denotes |
libraries |
T2814 |
1154-1157 |
VBP |
denotes |
are |
T2817 |
1158-1160 |
IN |
denotes |
of |
T2818 |
1161-1165 |
JJ |
denotes |
high |
T2819 |
1166-1173 |
NN |
denotes |
quality |
T2820 |
1173-1174 |
. |
denotes |
. |
T2821 |
1174-1328 |
sentence |
denotes |
Firstly, directional cloning was successful: only eight out of 1,430 cDNA sequences with any protein homology matched that protein on the reverse strand. |
T2822 |
1175-1182 |
RB |
denotes |
Firstly |
T2824 |
1182-1184 |
, |
denotes |
, |
T2825 |
1184-1195 |
JJ |
denotes |
directional |
T2826 |
1196-1203 |
NN |
denotes |
cloning |
T2827 |
1204-1207 |
VBD |
denotes |
was |
T2828 |
1208-1218 |
JJ |
denotes |
successful |
T2829 |
1218-1220 |
: |
denotes |
: |
T2830 |
1220-1224 |
RB |
denotes |
only |
T2832 |
1225-1230 |
CD |
denotes |
eight |
T2833 |
1231-1234 |
IN |
denotes |
out |
T2834 |
1235-1237 |
IN |
denotes |
of |
T2831 |
1238-1243 |
CD |
denotes |
1,430 |
T2836 |
1244-1248 |
NN |
denotes |
cDNA |
T2835 |
1249-1258 |
NNS |
denotes |
sequences |
T2837 |
1259-1263 |
IN |
denotes |
with |
T2838 |
1264-1267 |
DT |
denotes |
any |
T2840 |
1268-1275 |
NN |
denotes |
protein |
T2839 |
1276-1284 |
NN |
denotes |
homology |
T2823 |
1285-1292 |
VBD |
denotes |
matched |
T2841 |
1293-1297 |
DT |
denotes |
that |
T2842 |
1298-1305 |
NN |
denotes |
protein |
T2843 |
1306-1308 |
IN |
denotes |
on |
T2844 |
1309-1312 |
DT |
denotes |
the |
T2846 |
1313-1320 |
JJ |
denotes |
reverse |
T2845 |
1321-1327 |
NN |
denotes |
strand |
T2847 |
1327-1328 |
. |
denotes |
. |
T2848 |
1328-1640 |
sentence |
denotes |
Secondly, genomic contamination is rare: when the 83 olfactory receptor-containing sequences that had a 5' UTR of 400 bp or longer were aligned to genomic sequence, 80 spliced across at least one intron, leaving a maximum of three clones (3.6%) that potentially represent genomic contamination of the libraries. |
T2849 |
1329-1337 |
RB |
denotes |
Secondly |
T2851 |
1337-1339 |
, |
denotes |
, |
T2852 |
1339-1346 |
JJ |
denotes |
genomic |
T2853 |
1347-1360 |
NN |
denotes |
contamination |
T2854 |
1361-1363 |
VBZ |
denotes |
is |
T2855 |
1364-1368 |
JJ |
denotes |
rare |
T2856 |
1368-1370 |
: |
denotes |
: |
T2857 |
1370-1374 |
WRB |
denotes |
when |
T2859 |
1375-1378 |
DT |
denotes |
the |
T2861 |
1379-1381 |
CD |
denotes |
83 |
T2862 |
1382-1391 |
JJ |
denotes |
olfactory |
T2863 |
1392-1400 |
NN |
denotes |
receptor |
T2865 |
1400-1401 |
HYPH |
denotes |
- |
T2864 |
1401-1411 |
VBG |
denotes |
containing |
T2860 |
1412-1421 |
NNS |
denotes |
sequences |
T2866 |
1422-1426 |
WDT |
denotes |
that |
T2867 |
1427-1430 |
VBD |
denotes |
had |
T2868 |
1431-1432 |
DT |
denotes |
a |
T2870 |
1433-1434 |
CD |
denotes |
5 |
T2871 |
1434-1435 |
SYM |
denotes |
' |
T2869 |
1436-1439 |
NN |
denotes |
UTR |
T2872 |
1440-1442 |
IN |
denotes |
of |
T2873 |
1443-1446 |
CD |
denotes |
400 |
T2874 |
1447-1449 |
NNS |
denotes |
bp |
T2875 |
1450-1452 |
CC |
denotes |
or |
T2876 |
1453-1459 |
JJR |
denotes |
longer |
T2877 |
1460-1464 |
VBD |
denotes |
were |
T2858 |
1465-1472 |
VBN |
denotes |
aligned |
T2878 |
1473-1475 |
IN |
denotes |
to |
T2879 |
1476-1483 |
JJ |
denotes |
genomic |
T2880 |
1484-1492 |
NN |
denotes |
sequence |
T2881 |
1492-1494 |
, |
denotes |
, |
T2882 |
1494-1496 |
CD |
denotes |
80 |
T2850 |
1497-1504 |
VBN |
denotes |
spliced |
T2883 |
1505-1511 |
IN |
denotes |
across |
T2884 |
1512-1514 |
RB |
denotes |
at |
T2886 |
1515-1520 |
RBS |
denotes |
least |
T2885 |
1521-1524 |
CD |
denotes |
one |
T2887 |
1525-1531 |
NN |
denotes |
intron |
T2888 |
1531-1533 |
, |
denotes |
, |
T2889 |
1533-1540 |
VBG |
denotes |
leaving |
T2890 |
1541-1542 |
DT |
denotes |
a |
T2891 |
1543-1550 |
NN |
denotes |
maximum |
T2892 |
1551-1553 |
IN |
denotes |
of |
T2893 |
1554-1559 |
CD |
denotes |
three |
T2894 |
1560-1566 |
NNS |
denotes |
clones |
T2895 |
1567-1568 |
-LRB- |
denotes |
( |
T2897 |
1568-1571 |
CD |
denotes |
3.6 |
T2896 |
1571-1572 |
NN |
denotes |
% |
T2898 |
1572-1573 |
-RRB- |
denotes |
) |
T2899 |
1574-1578 |
WDT |
denotes |
that |
T2901 |
1579-1590 |
RB |
denotes |
potentially |
T2900 |
1591-1600 |
VBP |
denotes |
represent |
T2902 |
1601-1608 |
JJ |
denotes |
genomic |
T2903 |
1609-1622 |
NN |
denotes |
contamination |
T2904 |
1623-1625 |
IN |
denotes |
of |
T2905 |
1626-1629 |
DT |
denotes |
the |
T2906 |
1630-1639 |
NNS |
denotes |
libraries |
T2907 |
1639-1640 |
. |
denotes |
. |
T2908 |
1640-1861 |
sentence |
denotes |
Thirdly, most clones are of a reasonable length: although we did not determine whether clones are full-length, 881 of 1,264 (70%) olfactory receptor cDNAs contain the gene's start codon and at least some 5' UTR sequence. |
T2909 |
1641-1648 |
RB |
denotes |
Thirdly |
T2911 |
1648-1650 |
, |
denotes |
, |
T2912 |
1650-1654 |
JJS |
denotes |
most |
T2913 |
1655-1661 |
NNS |
denotes |
clones |
T2914 |
1662-1665 |
VBP |
denotes |
are |
T2915 |
1666-1668 |
IN |
denotes |
of |
T2916 |
1669-1670 |
DT |
denotes |
a |
T2918 |
1671-1681 |
JJ |
denotes |
reasonable |
T2917 |
1682-1688 |
NN |
denotes |
length |
T2919 |
1688-1690 |
: |
denotes |
: |
T2920 |
1690-1698 |
IN |
denotes |
although |
T2922 |
1699-1701 |
PRP |
denotes |
we |
T2923 |
1702-1705 |
VBD |
denotes |
did |
T2924 |
1706-1709 |
RB |
denotes |
not |
T2921 |
1710-1719 |
VB |
denotes |
determine |
T2925 |
1720-1727 |
IN |
denotes |
whether |
T2927 |
1728-1734 |
NNS |
denotes |
clones |
T2926 |
1735-1738 |
VBP |
denotes |
are |
T2928 |
1739-1743 |
JJ |
denotes |
full |
T2930 |
1743-1744 |
HYPH |
denotes |
- |
T2929 |
1744-1750 |
NN |
denotes |
length |
T2931 |
1750-1752 |
, |
denotes |
, |
T2932 |
1752-1755 |
CD |
denotes |
881 |
T2934 |
1756-1758 |
IN |
denotes |
of |
T2933 |
1759-1764 |
CD |
denotes |
1,264 |
T2936 |
1765-1766 |
-LRB- |
denotes |
( |
T2938 |
1766-1768 |
CD |
denotes |
70 |
T2937 |
1768-1769 |
NN |
denotes |
% |
T2939 |
1769-1770 |
-RRB- |
denotes |
) |
T2940 |
1771-1780 |
JJ |
denotes |
olfactory |
T2941 |
1781-1789 |
NN |
denotes |
receptor |
T2935 |
1790-1795 |
NNS |
denotes |
cDNAs |
T2910 |
1796-1803 |
VBP |
denotes |
contain |
T2942 |
1804-1807 |
DT |
denotes |
the |
T2943 |
1808-1812 |
NN |
denotes |
gene |
T2945 |
1812-1814 |
POS |
denotes |
's |
T2946 |
1815-1820 |
NN |
denotes |
start |
T2944 |
1821-1826 |
NN |
denotes |
codon |
T2947 |
1827-1830 |
CC |
denotes |
and |
T2948 |
1831-1833 |
RB |
denotes |
at |
T2949 |
1834-1839 |
RBS |
denotes |
least |
T2951 |
1840-1844 |
DT |
denotes |
some |
T2952 |
1845-1846 |
CD |
denotes |
5 |
T2954 |
1846-1847 |
SYM |
denotes |
' |
T2953 |
1848-1851 |
NN |
denotes |
UTR |
T2950 |
1852-1860 |
NN |
denotes |
sequence |
T2955 |
1860-1861 |
. |
denotes |
. |
T2956 |
1861-2048 |
sentence |
denotes |
In order to match cDNAs to their genomic counterparts, first we updated our catalog of mouse olfactory receptor genes [1] based on Celera's most recent genome assembly (Release 13) [29]. |
T2957 |
1862-1864 |
IN |
denotes |
In |
T2959 |
1865-1870 |
NN |
denotes |
order |
T2960 |
1871-1873 |
TO |
denotes |
to |
T2961 |
1874-1879 |
VB |
denotes |
match |
T2962 |
1880-1885 |
NNS |
denotes |
cDNAs |
T2963 |
1886-1888 |
IN |
denotes |
to |
T2964 |
1889-1894 |
PRP$ |
denotes |
their |
T2966 |
1895-1902 |
JJ |
denotes |
genomic |
T2965 |
1903-1915 |
NNS |
denotes |
counterparts |
T2967 |
1915-1917 |
, |
denotes |
, |
T2968 |
1917-1922 |
RB |
denotes |
first |
T2969 |
1923-1925 |
PRP |
denotes |
we |
T2958 |
1926-1933 |
VBD |
denotes |
updated |
T2970 |
1934-1937 |
PRP$ |
denotes |
our |
T2971 |
1938-1945 |
NN |
denotes |
catalog |
T2972 |
1946-1948 |
IN |
denotes |
of |
T2973 |
1949-1954 |
NN |
denotes |
mouse |
T2975 |
1955-1964 |
JJ |
denotes |
olfactory |
T2976 |
1965-1973 |
NN |
denotes |
receptor |
T2974 |
1974-1979 |
NNS |
denotes |
genes |
T2977 |
1980-1981 |
-LRB- |
denotes |
[ |
T2978 |
1981-1982 |
CD |
denotes |
1 |
T2979 |
1982-1983 |
-RRB- |
denotes |
] |
T2980 |
1984-1989 |
VBN |
denotes |
based |
T2981 |
1990-1992 |
IN |
denotes |
on |
T2982 |
1993-1999 |
NNP |
denotes |
Celera |
T2984 |
1999-2001 |
POS |
denotes |
's |
T2985 |
2002-2006 |
RBS |
denotes |
most |
T2986 |
2007-2013 |
JJ |
denotes |
recent |
T2987 |
2014-2020 |
NN |
denotes |
genome |
T2983 |
2021-2029 |
NN |
denotes |
assembly |
T2988 |
2030-2031 |
-LRB- |
denotes |
( |
T2989 |
2031-2038 |
NN |
denotes |
Release |
T2990 |
2039-2041 |
CD |
denotes |
13 |
T2991 |
2041-2042 |
-RRB- |
denotes |
) |
T2992 |
2043-2044 |
-LRB- |
denotes |
[ |
T2993 |
2044-2046 |
CD |
denotes |
29 |
T2994 |
2046-2047 |
-RRB- |
denotes |
] |
T2995 |
2047-2048 |
. |
denotes |
. |
T2996 |
2048-2153 |
sentence |
denotes |
Previous reports of the mouse olfactory receptor repertoire [1,2] were based on the Release 12 assembly. |
T2997 |
2049-2057 |
JJ |
denotes |
Previous |
T2998 |
2058-2065 |
NNS |
denotes |
reports |
T3000 |
2066-2068 |
IN |
denotes |
of |
T3001 |
2069-2072 |
DT |
denotes |
the |
T3003 |
2073-2078 |
NN |
denotes |
mouse |
T3005 |
2079-2088 |
JJ |
denotes |
olfactory |
T3004 |
2089-2097 |
NN |
denotes |
receptor |
T3002 |
2098-2108 |
NN |
denotes |
repertoire |
T3006 |
2109-2110 |
-LRB- |
denotes |
[ |
T3008 |
2110-2111 |
CD |
denotes |
1 |
T3009 |
2111-2112 |
, |
denotes |
, |
T3007 |
2112-2113 |
CD |
denotes |
2 |
T3010 |
2113-2114 |
-RRB- |
denotes |
] |
T3011 |
2115-2119 |
VBD |
denotes |
were |
T2999 |
2120-2125 |
VBN |
denotes |
based |
T3012 |
2126-2128 |
IN |
denotes |
on |
T3013 |
2129-2132 |
DT |
denotes |
the |
T3015 |
2133-2140 |
NN |
denotes |
Release |
T3016 |
2141-2143 |
CD |
denotes |
12 |
T3014 |
2144-2152 |
NN |
denotes |
assembly |
T3017 |
2152-2153 |
. |
denotes |
. |
T3018 |
2153-2257 |
sentence |
denotes |
Release 13 consists of fewer, longer scaffold sequences containing fewer, smaller gaps than Release 12. |
T3019 |
2154-2161 |
NN |
denotes |
Release |
T3021 |
2162-2164 |
CD |
denotes |
13 |
T3020 |
2165-2173 |
VBZ |
denotes |
consists |
T3022 |
2174-2176 |
IN |
denotes |
of |
T3023 |
2177-2182 |
JJR |
denotes |
fewer |
T3025 |
2182-2184 |
, |
denotes |
, |
T3024 |
2184-2190 |
JJR |
denotes |
longer |
T3027 |
2191-2199 |
NN |
denotes |
scaffold |
T3026 |
2200-2209 |
NNS |
denotes |
sequences |
T3028 |
2210-2220 |
VBG |
denotes |
containing |
T3029 |
2221-2226 |
JJR |
denotes |
fewer |
T3031 |
2226-2228 |
, |
denotes |
, |
T3030 |
2228-2235 |
JJR |
denotes |
smaller |
T3032 |
2236-2240 |
NNS |
denotes |
gaps |
T3033 |
2241-2245 |
IN |
denotes |
than |
T3034 |
2246-2253 |
NN |
denotes |
Release |
T3035 |
2254-2256 |
CD |
denotes |
12 |
T3036 |
2256-2257 |
. |
denotes |
. |
T3037 |
2257-2592 |
sentence |
denotes |
Using the BLAST-based methods detailed previously [1], we identified 1,490 olfactory receptor sequences in the new assembly, including 1,107 intact olfactory receptor genes (compared to 866 intact olfactory receptors in the old assembly) reflecting the reduced sequence error rate and increased coverage of the new assembly (Table 1). |
T3038 |
2258-2263 |
VBG |
denotes |
Using |
T3040 |
2264-2267 |
DT |
denotes |
the |
T3042 |
2268-2273 |
NN |
denotes |
BLAST |
T3044 |
2273-2274 |
HYPH |
denotes |
- |
T3043 |
2274-2279 |
VBN |
denotes |
based |
T3041 |
2280-2287 |
NNS |
denotes |
methods |
T3045 |
2288-2296 |
VBN |
denotes |
detailed |
T3046 |
2297-2307 |
RB |
denotes |
previously |
T3047 |
2308-2309 |
-LRB- |
denotes |
[ |
T3048 |
2309-2310 |
CD |
denotes |
1 |
T3049 |
2310-2311 |
-RRB- |
denotes |
] |
T3050 |
2311-2313 |
, |
denotes |
, |
T3051 |
2313-2315 |
PRP |
denotes |
we |
T3039 |
2316-2326 |
VBD |
denotes |
identified |
T3052 |
2327-2332 |
CD |
denotes |
1,490 |
T3054 |
2333-2342 |
JJ |
denotes |
olfactory |
T3055 |
2343-2351 |
NN |
denotes |
receptor |
T3053 |
2352-2361 |
NNS |
denotes |
sequences |
T3056 |
2362-2364 |
IN |
denotes |
in |
T3057 |
2365-2368 |
DT |
denotes |
the |
T3059 |
2369-2372 |
JJ |
denotes |
new |
T3058 |
2373-2381 |
NN |
denotes |
assembly |
T3060 |
2381-2383 |
, |
denotes |
, |
T3061 |
2383-2392 |
VBG |
denotes |
including |
T3062 |
2393-2398 |
CD |
denotes |
1,107 |
T3064 |
2399-2405 |
JJ |
denotes |
intact |
T3065 |
2406-2415 |
JJ |
denotes |
olfactory |
T3066 |
2416-2424 |
NN |
denotes |
receptor |
T3063 |
2425-2430 |
NNS |
denotes |
genes |
T3067 |
2431-2432 |
-LRB- |
denotes |
( |
T3068 |
2432-2440 |
VBN |
denotes |
compared |
T3069 |
2441-2443 |
IN |
denotes |
to |
T3070 |
2444-2447 |
CD |
denotes |
866 |
T3072 |
2448-2454 |
JJ |
denotes |
intact |
T3073 |
2455-2464 |
JJ |
denotes |
olfactory |
T3071 |
2465-2474 |
NNS |
denotes |
receptors |
T3074 |
2475-2477 |
IN |
denotes |
in |
T3075 |
2478-2481 |
DT |
denotes |
the |
T3077 |
2482-2485 |
JJ |
denotes |
old |
T3076 |
2486-2494 |
NN |
denotes |
assembly |
T3078 |
2494-2495 |
-RRB- |
denotes |
) |
T3079 |
2496-2506 |
VBG |
denotes |
reflecting |
T3080 |
2507-2510 |
DT |
denotes |
the |
T3082 |
2511-2518 |
VBN |
denotes |
reduced |
T3083 |
2519-2527 |
NN |
denotes |
sequence |
T3084 |
2528-2533 |
NN |
denotes |
error |
T3081 |
2534-2538 |
NN |
denotes |
rate |
T3085 |
2539-2542 |
CC |
denotes |
and |
T3086 |
2543-2552 |
VBN |
denotes |
increased |
T3087 |
2553-2561 |
NN |
denotes |
coverage |
T3088 |
2562-2564 |
IN |
denotes |
of |
T3089 |
2565-2568 |
DT |
denotes |
the |
T3091 |
2569-2572 |
JJ |
denotes |
new |
T3090 |
2573-2581 |
NN |
denotes |
assembly |
T3092 |
2582-2583 |
-LRB- |
denotes |
( |
T3093 |
2583-2588 |
NN |
denotes |
Table |
T3094 |
2589-2590 |
CD |
denotes |
1 |
T3095 |
2590-2591 |
-RRB- |
denotes |
) |
T3096 |
2591-2592 |
. |
denotes |
. |
T3097 |
2592-2812 |
sentence |
denotes |
We created a local database of genomic sequences including all olfactory receptor loci and 0.5 Mb flanking sequences (if available) and compared each cDNA sequence to this 'olfactory subgenome' database using sim4 [30]. |
T3098 |
2593-2595 |
PRP |
denotes |
We |
T3099 |
2596-2603 |
VBD |
denotes |
created |
T3100 |
2604-2605 |
DT |
denotes |
a |
T3102 |
2606-2611 |
JJ |
denotes |
local |
T3101 |
2612-2620 |
NN |
denotes |
database |
T3103 |
2621-2623 |
IN |
denotes |
of |
T3104 |
2624-2631 |
JJ |
denotes |
genomic |
T3105 |
2632-2641 |
NNS |
denotes |
sequences |
T3106 |
2642-2651 |
VBG |
denotes |
including |
T3107 |
2652-2655 |
DT |
denotes |
all |
T3109 |
2656-2665 |
JJ |
denotes |
olfactory |
T3110 |
2666-2674 |
NN |
denotes |
receptor |
T3108 |
2675-2679 |
NNS |
denotes |
loci |
T3111 |
2680-2683 |
CC |
denotes |
and |
T3112 |
2684-2687 |
CD |
denotes |
0.5 |
T3113 |
2688-2690 |
NN |
denotes |
Mb |
T3115 |
2691-2699 |
NN |
denotes |
flanking |
T3114 |
2700-2709 |
NNS |
denotes |
sequences |
T3116 |
2710-2711 |
-LRB- |
denotes |
( |
T3118 |
2711-2713 |
IN |
denotes |
if |
T3117 |
2714-2723 |
JJ |
denotes |
available |
T3119 |
2723-2724 |
-RRB- |
denotes |
) |
T3120 |
2725-2728 |
CC |
denotes |
and |
T3121 |
2729-2737 |
VBN |
denotes |
compared |
T3122 |
2738-2742 |
DT |
denotes |
each |
T3124 |
2743-2747 |
NN |
denotes |
cDNA |
T3123 |
2748-2756 |
NN |
denotes |
sequence |
T3125 |
2757-2759 |
IN |
denotes |
to |
T3126 |
2760-2764 |
DT |
denotes |
this |
T3128 |
2765-2766 |
`` |
denotes |
' |
T3129 |
2766-2775 |
JJ |
denotes |
olfactory |
T3130 |
2776-2785 |
NN |
denotes |
subgenome |
T3131 |
2785-2786 |
'' |
denotes |
' |
T3127 |
2787-2795 |
NN |
denotes |
database |
T3132 |
2796-2801 |
VBG |
denotes |
using |
T3133 |
2802-2806 |
NN |
denotes |
sim4 |
T3134 |
2807-2808 |
-LRB- |
denotes |
[ |
T3135 |
2808-2810 |
CD |
denotes |
30 |
T3136 |
2810-2811 |
-RRB- |
denotes |
] |
T3137 |
2811-2812 |
. |
denotes |
. |
T3138 |
2812-2969 |
sentence |
denotes |
cDNAs were assigned to individual genes based on their best match to an olfactory receptor coding region or its upstream region (see Materials and methods). |
T3139 |
2813-2818 |
NNS |
denotes |
cDNAs |
T3141 |
2819-2823 |
VBD |
denotes |
were |
T3140 |
2824-2832 |
VBN |
denotes |
assigned |
T3142 |
2833-2835 |
IN |
denotes |
to |
T3143 |
2836-2846 |
JJ |
denotes |
individual |
T3144 |
2847-2852 |
NNS |
denotes |
genes |
T3145 |
2853-2858 |
VBN |
denotes |
based |
T3146 |
2859-2861 |
IN |
denotes |
on |
T3147 |
2862-2867 |
PRP$ |
denotes |
their |
T3149 |
2868-2872 |
JJS |
denotes |
best |
T3148 |
2873-2878 |
NN |
denotes |
match |
T3150 |
2879-2881 |
IN |
denotes |
to |
T3151 |
2882-2884 |
DT |
denotes |
an |
T3153 |
2885-2894 |
JJ |
denotes |
olfactory |
T3154 |
2895-2903 |
NN |
denotes |
receptor |
T3155 |
2904-2910 |
NN |
denotes |
coding |
T3152 |
2911-2917 |
NN |
denotes |
region |
T3156 |
2918-2920 |
CC |
denotes |
or |
T3157 |
2921-2924 |
PRP$ |
denotes |
its |
T3159 |
2925-2933 |
JJ |
denotes |
upstream |
T3158 |
2934-2940 |
NN |
denotes |
region |
T3160 |
2941-2942 |
-LRB- |
denotes |
( |
T3161 |
2942-2945 |
VB |
denotes |
see |
T3162 |
2946-2955 |
NNS |
denotes |
Materials |
T3163 |
2956-2959 |
CC |
denotes |
and |
T3164 |
2960-2967 |
NNS |
denotes |
methods |
T3165 |
2967-2968 |
-RRB- |
denotes |
) |
T3166 |
2968-2969 |
. |
denotes |
. |
T3167 |
2969-3260 |
sentence |
denotes |
Of the 1,264 olfactory receptor cDNAs, 1,176 matched a total of 419 olfactory receptor genes; the remaining cDNAs either matched an olfactory receptor below our 96% nucleotide identity threshold or had ambiguous matches encompassing more than one olfactory receptor gene region (see below). |
T3168 |
2970-2972 |
IN |
denotes |
Of |
T3170 |
2973-2976 |
DT |
denotes |
the |
T3172 |
2977-2982 |
CD |
denotes |
1,264 |
T3173 |
2983-2992 |
JJ |
denotes |
olfactory |
T3174 |
2993-3001 |
NN |
denotes |
receptor |
T3171 |
3002-3007 |
NNS |
denotes |
cDNAs |
T3175 |
3007-3009 |
, |
denotes |
, |
T3176 |
3009-3014 |
CD |
denotes |
1,176 |
T3169 |
3015-3022 |
VBD |
denotes |
matched |
T3178 |
3023-3024 |
DT |
denotes |
a |
T3179 |
3025-3030 |
NN |
denotes |
total |
T3180 |
3031-3033 |
IN |
denotes |
of |
T3181 |
3034-3037 |
CD |
denotes |
419 |
T3183 |
3038-3047 |
JJ |
denotes |
olfactory |
T3184 |
3048-3056 |
NN |
denotes |
receptor |
T3182 |
3057-3062 |
NNS |
denotes |
genes |
T3185 |
3062-3063 |
: |
denotes |
; |
T3186 |
3064-3067 |
DT |
denotes |
the |
T3188 |
3068-3077 |
VBG |
denotes |
remaining |
T3187 |
3078-3083 |
NNS |
denotes |
cDNAs |
T3189 |
3084-3090 |
CC |
denotes |
either |
T3177 |
3091-3098 |
VBD |
denotes |
matched |
T3190 |
3099-3101 |
DT |
denotes |
an |
T3192 |
3102-3111 |
JJ |
denotes |
olfactory |
T3191 |
3112-3120 |
NN |
denotes |
receptor |
T3193 |
3121-3126 |
IN |
denotes |
below |
T3194 |
3127-3130 |
PRP$ |
denotes |
our |
T3196 |
3131-3133 |
CD |
denotes |
96 |
T3197 |
3133-3134 |
NN |
denotes |
% |
T3199 |
3135-3145 |
NN |
denotes |
nucleotide |
T3198 |
3146-3154 |
NN |
denotes |
identity |
T3195 |
3155-3164 |
NN |
denotes |
threshold |
T3200 |
3165-3167 |
CC |
denotes |
or |
T3201 |
3168-3171 |
VBD |
denotes |
had |
T3202 |
3172-3181 |
JJ |
denotes |
ambiguous |
T3203 |
3182-3189 |
NNS |
denotes |
matches |
T3204 |
3190-3202 |
VBG |
denotes |
encompassing |
T3205 |
3203-3207 |
JJR |
denotes |
more |
T3207 |
3208-3212 |
IN |
denotes |
than |
T3206 |
3213-3216 |
CD |
denotes |
one |
T3209 |
3217-3226 |
JJ |
denotes |
olfactory |
T3210 |
3227-3235 |
NN |
denotes |
receptor |
T3211 |
3236-3240 |
NN |
denotes |
gene |
T3208 |
3241-3247 |
NN |
denotes |
region |
T3212 |
3248-3249 |
-LRB- |
denotes |
( |
T3213 |
3249-3252 |
VB |
denotes |
see |
T3214 |
3253-3258 |
RB |
denotes |
below |
T3215 |
3258-3259 |
-RRB- |
denotes |
) |
T3216 |
3259-3260 |
. |
denotes |
. |
R1390 |
T2624 |
T2625 |
advmod |
At,419 |
R1391 |
T2625 |
T2627 |
nummod |
419,genes |
R1392 |
T2626 |
T2625 |
advmod |
least,419 |
R1393 |
T2627 |
T2631 |
nsubjpass |
genes,expressed |
R1394 |
T2628 |
T2627 |
nmod |
mouse,genes |
R1395 |
T2629 |
T2630 |
amod |
olfactory,receptor |
R1396 |
T2630 |
T2627 |
compound |
receptor,genes |
R1397 |
T2632 |
T2631 |
auxpass |
are,expressed |
R1398 |
T2633 |
T2631 |
prep |
in,expressed |
R1399 |
T2634 |
T2635 |
det |
the,epithelium |
R1400 |
T2635 |
T2633 |
pobj |
epithelium,in |
R1401 |
T2636 |
T2635 |
amod |
olfactory,epithelium |
R1402 |
T2638 |
T2639 |
nsubj |
We,isolated |
R1403 |
T2640 |
T2639 |
aux |
have,isolated |
R1404 |
T2641 |
T2642 |
nummod |
"1,264",clones |
R1405 |
T2642 |
T2639 |
dobj |
clones,isolated |
R1406 |
T2643 |
T2644 |
amod |
olfactory,receptor |
R1407 |
T2644 |
T2645 |
compound |
receptor,cDNA |
R1408 |
T2645 |
T2642 |
compound |
cDNA,clones |
R1409 |
T2646 |
T2639 |
punct |
", ",isolated |
R1410 |
T2647 |
T2648 |
dep |
which,confirm |
R1411 |
T2648 |
T2639 |
ccomp |
confirm,isolated |
R1412 |
T2649 |
T2648 |
advmod |
together,confirm |
R1413 |
T2650 |
T2651 |
det |
the,expression |
R1414 |
T2651 |
T2648 |
dobj |
expression,confirm |
R1415 |
T2652 |
T2653 |
amod |
olfactory,epithelial |
R1416 |
T2653 |
T2651 |
amod |
epithelial,expression |
R1417 |
T2654 |
T2651 |
prep |
of,expression |
R1418 |
T2655 |
T2656 |
nummod |
419,genes |
R1419 |
T2656 |
T2654 |
pobj |
genes,of |
R1420 |
T2657 |
T2656 |
amod |
annotated,genes |
R1421 |
T2658 |
T2659 |
amod |
olfactory,receptor |
R1422 |
T2659 |
T2656 |
compound |
receptor,genes |
R1423 |
T2660 |
T2639 |
punct |
.,isolated |
R1424 |
T2662 |
T2663 |
nsubj |
We,used |
R1425 |
T2664 |
T2665 |
amod |
low,stringency |
R1426 |
T2665 |
T2667 |
compound |
stringency,hybridization |
R1427 |
T2666 |
T2665 |
punct |
-,stringency |
R1428 |
T2667 |
T2663 |
dobj |
hybridization,used |
R1429 |
T2668 |
T2667 |
prep |
with,hybridization |
R1430 |
T2669 |
T2670 |
amod |
degenerate,probes |
R1431 |
T2670 |
T2668 |
pobj |
probes,with |
R1432 |
T2671 |
T2672 |
amod |
olfactory,receptor |
R1433 |
T2672 |
T2670 |
compound |
receptor,probes |
R1434 |
T2673 |
T2670 |
compound |
DNA,probes |
R1435 |
T2674 |
T2675 |
aux |
to,screen |
R1436 |
T2675 |
T2663 |
advcl |
screen,used |
R1437 |
T2676 |
T2677 |
advmod |
around,"4,100,000" |
R1438 |
T2677 |
T2678 |
nummod |
"4,100,000",units |
R1439 |
T2678 |
T2675 |
dobj |
units,screen |
R1440 |
T2679 |
T2680 |
npadvmod |
plaque,forming |
R1441 |
T2680 |
T2678 |
amod |
forming,units |
R1442 |
T2681 |
T2680 |
punct |
-,forming |
R1443 |
T2682 |
T2678 |
punct |
(,units |
R1444 |
T2683 |
T2678 |
appos |
pfu,units |
R1445 |
T2684 |
T2678 |
punct |
),units |
R1446 |
T2685 |
T2678 |
prep |
of,units |
R1447 |
T2686 |
T2687 |
det |
an,library |
R1448 |
T2687 |
T2685 |
pobj |
library,of |
R1449 |
T2688 |
T2689 |
amod |
adult,mouse |
R1450 |
T2689 |
T2690 |
nmod |
mouse,cDNA |
R1451 |
T2690 |
T2687 |
compound |
cDNA,library |
R1452 |
T2691 |
T2692 |
amod |
olfactory,epithelium |
R1453 |
T2692 |
T2690 |
compound |
epithelium,cDNA |
R1454 |
T2693 |
T2678 |
cc |
and,units |
R1455 |
T2694 |
T2695 |
advmod |
around,"640,000" |
R1456 |
T2695 |
T2696 |
nummod |
"640,000",pfu |
R1457 |
T2696 |
T2678 |
conj |
pfu,units |
R1458 |
T2697 |
T2696 |
prep |
of,pfu |
R1459 |
T2698 |
T2699 |
det |
an,library |
R1460 |
T2699 |
T2697 |
pobj |
library,of |
R1461 |
T2700 |
T2701 |
amod |
embryonic,epithelium |
R1462 |
T2701 |
T2699 |
compound |
epithelium,library |
R1463 |
T2702 |
T2701 |
amod |
olfactory,epithelium |
R1464 |
T2703 |
T2663 |
punct |
.,used |
R1465 |
T2705 |
T2706 |
nsubj |
We,obtained |
R1466 |
T2707 |
T2706 |
dobj |
sequences,obtained |
R1467 |
T2708 |
T2706 |
prep |
from,obtained |
R1468 |
T2709 |
T2710 |
nummod |
"1,715",cDNAs |
R1469 |
T2710 |
T2708 |
pobj |
cDNAs,from |
R1470 |
T2711 |
T2712 |
npadvmod |
hybridization,positive |
R1471 |
T2712 |
T2710 |
amod |
positive,cDNAs |
R1472 |
T2713 |
T2712 |
punct |
-,positive |
R1473 |
T2714 |
T2706 |
prep |
following,obtained |
R1474 |
T2715 |
T2716 |
amod |
secondary,screens |
R1475 |
T2716 |
T2714 |
pobj |
screens,following |
R1476 |
T2717 |
T2718 |
aux |
to,isolate |
R1477 |
T2718 |
T2716 |
advcl |
isolate,screens |
R1478 |
T2719 |
T2720 |
amod |
single,clones |
R1479 |
T2720 |
T2718 |
dobj |
clones,isolate |
R1480 |
T2721 |
T2706 |
punct |
.,obtained |
R1481 |
T2723 |
T2724 |
prep |
Of,yielded |
R1482 |
T2725 |
T2726 |
det |
these,clones |
R1483 |
T2726 |
T2723 |
pobj |
clones,Of |
R1484 |
T2727 |
T2724 |
punct |
", ",yielded |
R1485 |
T2728 |
T2724 |
nsubj |
"1,264",yielded |
R1486 |
T2729 |
T2730 |
amod |
olfactory,receptor |
R1487 |
T2730 |
T2731 |
npadvmod |
receptor,containing |
R1488 |
T2731 |
T2733 |
amod |
containing,sequences |
R1489 |
T2732 |
T2731 |
punct |
-,containing |
R1490 |
T2733 |
T2724 |
dobj |
sequences,yielded |
R1491 |
T2734 |
T2724 |
punct |
.,yielded |
R1492 |
T2736 |
T2737 |
det |
The,rate |
R1493 |
T2737 |
T2743 |
nsubj |
rate,is |
R1494 |
T2738 |
T2739 |
nummod |
26,% |
R1495 |
T2739 |
T2737 |
nmod |
%,rate |
R1496 |
T2740 |
T2741 |
amod |
false,positive |
R1497 |
T2741 |
T2737 |
amod |
positive,rate |
R1498 |
T2742 |
T2741 |
punct |
-,positive |
R1499 |
T2744 |
T2745 |
det |
a,consequence |
R1500 |
T2745 |
T2743 |
attr |
consequence,is |
R1501 |
T2746 |
T2745 |
prep |
of,consequence |
R1502 |
T2747 |
T2746 |
pcomp |
using,of |
R1503 |
T2748 |
T2749 |
amod |
low,stringency |
R1504 |
T2749 |
T2751 |
compound |
stringency,hybridization |
R1505 |
T2750 |
T2749 |
punct |
-,stringency |
R1506 |
T2751 |
T2747 |
dobj |
hybridization,using |
R1507 |
T2752 |
T2753 |
aux |
to,obtain |
R1508 |
T2753 |
T2747 |
advcl |
obtain,using |
R1509 |
T2754 |
T2755 |
amod |
maximal,sensitivity |
R1510 |
T2755 |
T2753 |
dobj |
sensitivity,obtain |
R1511 |
T2756 |
T2743 |
punct |
.,is |
R1512 |
T2758 |
T2759 |
csubj |
Continuing,resulted |
R1513 |
T2759 |
T2764 |
ccomp |
resulted,represented |
R1514 |
T2760 |
T2761 |
det |
the,screen |
R1515 |
T2761 |
T2758 |
dobj |
screen,Continuing |
R1516 |
T2762 |
T2759 |
aux |
would,resulted |
R1517 |
T2763 |
T2759 |
aux |
have,resulted |
R1518 |
T2765 |
T2759 |
prep |
in,resulted |
R1519 |
T2766 |
T2765 |
pobj |
cDNAs,in |
R1520 |
T2767 |
T2766 |
prep |
from,cDNAs |
R1521 |
T2768 |
T2769 |
amod |
additional,receptors |
R1522 |
T2769 |
T2767 |
pobj |
receptors,from |
R1523 |
T2770 |
T2769 |
amod |
olfactory,receptors |
R1524 |
T2771 |
T2759 |
punct |
", ",resulted |
R1525 |
T2772 |
T2759 |
cc |
but,resulted |
R1526 |
T2773 |
T2774 |
nsubj |
we,reached |
R1527 |
T2774 |
T2759 |
conj |
reached,resulted |
R1528 |
T2775 |
T2776 |
det |
a,point |
R1529 |
T2776 |
T2774 |
dobj |
point,reached |
R1530 |
T2777 |
T2776 |
prep |
of,point |
R1531 |
T2778 |
T2779 |
amod |
limiting,returns |
R1532 |
T2779 |
T2777 |
pobj |
returns,of |
R1533 |
T2780 |
T2764 |
punct |
: ,represented |
R1534 |
T2781 |
T2782 |
poss |
our,batch |
R1535 |
T2782 |
T2764 |
nsubj |
batch,represented |
R1536 |
T2783 |
T2782 |
amod |
final,batch |
R1537 |
T2784 |
T2782 |
prep |
of,batch |
R1538 |
T2785 |
T2786 |
nummod |
45,sequences |
R1539 |
T2786 |
T2784 |
pobj |
sequences,of |
R1540 |
T2787 |
T2788 |
amod |
olfactory,receptor |
R1541 |
T2788 |
T2789 |
npadvmod |
receptor,positive |
R1542 |
T2789 |
T2786 |
amod |
positive,sequences |
R1543 |
T2790 |
T2789 |
punct |
-,positive |
R1544 |
T2791 |
T2792 |
nummod |
33,receptors |
R1545 |
T2792 |
T2764 |
dobj |
receptors,represented |
R1546 |
T2793 |
T2792 |
amod |
different,receptors |
R1547 |
T2794 |
T2792 |
amod |
olfactory,receptors |
R1548 |
T2795 |
T2792 |
punct |
", ",receptors |
R1549 |
T2796 |
T2797 |
prep |
of,encountered |
R1550 |
T2797 |
T2792 |
relcl |
encountered,receptors |
R1551 |
T2798 |
T2796 |
pobj |
which,of |
R1552 |
T2799 |
T2800 |
advmod |
only,four |
R1553 |
T2800 |
T2797 |
nsubjpass |
four,encountered |
R1554 |
T2801 |
T2797 |
aux |
had,encountered |
R1555 |
T2802 |
T2797 |
neg |
not,encountered |
R1556 |
T2803 |
T2797 |
auxpass |
been,encountered |
R1557 |
T2804 |
T2797 |
advmod |
previously,encountered |
R1558 |
T2805 |
T2797 |
prep |
in,encountered |
R1559 |
T2806 |
T2807 |
poss |
our,screen |
R1560 |
T2807 |
T2805 |
pobj |
screen,in |
R1561 |
T2808 |
T2764 |
punct |
.,represented |
R1562 |
T2810 |
T2811 |
compound |
Sequence,analysis |
R1563 |
T2811 |
T2812 |
nsubj |
analysis,shows |
R1564 |
T2813 |
T2814 |
mark |
that,are |
R1565 |
T2814 |
T2812 |
ccomp |
are,shows |
R1566 |
T2815 |
T2816 |
det |
the,libraries |
R1567 |
T2816 |
T2814 |
nsubj |
libraries,are |
R1568 |
T2817 |
T2814 |
prep |
of,are |
R1569 |
T2818 |
T2819 |
amod |
high,quality |
R1570 |
T2819 |
T2817 |
pobj |
quality,of |
R1571 |
T2820 |
T2812 |
punct |
.,shows |
R1572 |
T2822 |
T2823 |
advmod |
Firstly,matched |
R1573 |
T2824 |
T2823 |
punct |
", ",matched |
R1574 |
T2825 |
T2826 |
amod |
directional,cloning |
R1575 |
T2826 |
T2827 |
nsubj |
cloning,was |
R1576 |
T2827 |
T2823 |
ccomp |
was,matched |
R1577 |
T2828 |
T2827 |
acomp |
successful,was |
R1578 |
T2829 |
T2823 |
punct |
: ,matched |
R1579 |
T2830 |
T2831 |
advmod |
only,"1,430" |
R1580 |
T2831 |
T2835 |
nummod |
"1,430",sequences |
R1581 |
T2832 |
T2831 |
quantmod |
eight,"1,430" |
R1582 |
T2833 |
T2831 |
quantmod |
out,"1,430" |
R1583 |
T2834 |
T2831 |
quantmod |
of,"1,430" |
R1584 |
T2835 |
T2823 |
nsubj |
sequences,matched |
R1585 |
T2836 |
T2835 |
compound |
cDNA,sequences |
R1586 |
T2837 |
T2835 |
prep |
with,sequences |
R1587 |
T2838 |
T2839 |
det |
any,homology |
R1588 |
T2839 |
T2837 |
pobj |
homology,with |
R1589 |
T2840 |
T2839 |
compound |
protein,homology |
R1590 |
T2841 |
T2842 |
det |
that,protein |
R1591 |
T2842 |
T2823 |
dobj |
protein,matched |
R1592 |
T2843 |
T2823 |
prep |
on,matched |
R1593 |
T2844 |
T2845 |
det |
the,strand |
R1594 |
T2845 |
T2843 |
pobj |
strand,on |
R1595 |
T2846 |
T2845 |
amod |
reverse,strand |
R1596 |
T2847 |
T2823 |
punct |
.,matched |
R1597 |
T2849 |
T2850 |
advmod |
Secondly,spliced |
R1598 |
T2851 |
T2850 |
punct |
", ",spliced |
R1599 |
T2852 |
T2853 |
amod |
genomic,contamination |
R1600 |
T2853 |
T2854 |
nsubj |
contamination,is |
R1601 |
T2854 |
T2850 |
ccomp |
is,spliced |
R1602 |
T2855 |
T2854 |
acomp |
rare,is |
R1603 |
T2856 |
T2850 |
punct |
: ,spliced |
R1604 |
T2857 |
T2858 |
advmod |
when,aligned |
R1605 |
T2858 |
T2850 |
advcl |
aligned,spliced |
R1606 |
T2859 |
T2860 |
det |
the,sequences |
R1607 |
T2860 |
T2858 |
nsubjpass |
sequences,aligned |
R1608 |
T2861 |
T2860 |
nummod |
83,sequences |
R1609 |
T2862 |
T2863 |
amod |
olfactory,receptor |
R1610 |
T2863 |
T2864 |
npadvmod |
receptor,containing |
R1611 |
T2864 |
T2860 |
amod |
containing,sequences |
R1612 |
T2865 |
T2864 |
punct |
-,containing |
R1613 |
T2866 |
T2867 |
dep |
that,had |
R1614 |
T2867 |
T2860 |
relcl |
had,sequences |
R1615 |
T2868 |
T2869 |
det |
a,UTR |
R1616 |
T2869 |
T2867 |
dobj |
UTR,had |
R1617 |
T2870 |
T2869 |
nummod |
5,UTR |
R1618 |
T2871 |
T2870 |
punct |
',5 |
R1619 |
T2872 |
T2869 |
prep |
of,UTR |
R1620 |
T2873 |
T2874 |
nummod |
400,bp |
R1621 |
T2874 |
T2872 |
pobj |
bp,of |
R1622 |
T2875 |
T2876 |
cc |
or,longer |
R1623 |
T2876 |
T2874 |
nummod |
longer,bp |
R1624 |
T2877 |
T2858 |
auxpass |
were,aligned |
R1625 |
T2878 |
T2858 |
prep |
to,aligned |
R1626 |
T2879 |
T2880 |
amod |
genomic,sequence |
R1627 |
T2880 |
T2878 |
pobj |
sequence,to |
R1628 |
T2881 |
T2850 |
punct |
", ",spliced |
R1629 |
T2882 |
T2850 |
nsubj |
80,spliced |
R1630 |
T2883 |
T2850 |
prep |
across,spliced |
R1631 |
T2884 |
T2885 |
advmod |
at,one |
R1632 |
T2885 |
T2887 |
nummod |
one,intron |
R1633 |
T2886 |
T2885 |
advmod |
least,one |
R1634 |
T2887 |
T2883 |
pobj |
intron,across |
R1635 |
T2888 |
T2850 |
punct |
", ",spliced |
R1636 |
T2889 |
T2850 |
advcl |
leaving,spliced |
R1637 |
T2890 |
T2891 |
det |
a,maximum |
R1638 |
T2891 |
T2889 |
dobj |
maximum,leaving |
R1639 |
T2892 |
T2891 |
prep |
of,maximum |
R1640 |
T2893 |
T2894 |
nummod |
three,clones |
R1641 |
T2894 |
T2892 |
pobj |
clones,of |
R1642 |
T2895 |
T2896 |
punct |
(,% |
R1643 |
T2896 |
T2894 |
parataxis |
%,clones |
R1644 |
T2897 |
T2896 |
nummod |
3.6,% |
R1645 |
T2898 |
T2896 |
punct |
),% |
R1646 |
T2899 |
T2900 |
dep |
that,represent |
R1647 |
T2900 |
T2894 |
relcl |
represent,clones |
R1648 |
T2901 |
T2900 |
advmod |
potentially,represent |
R1649 |
T2902 |
T2903 |
amod |
genomic,contamination |
R1650 |
T2903 |
T2900 |
dobj |
contamination,represent |
R1651 |
T2904 |
T2903 |
prep |
of,contamination |
R1652 |
T2905 |
T2906 |
det |
the,libraries |
R1653 |
T2906 |
T2904 |
pobj |
libraries,of |
R1654 |
T2907 |
T2850 |
punct |
.,spliced |
R1655 |
T2909 |
T2910 |
advmod |
Thirdly,contain |
R1656 |
T2911 |
T2910 |
punct |
", ",contain |
R1657 |
T2912 |
T2913 |
amod |
most,clones |
R1658 |
T2913 |
T2914 |
nsubj |
clones,are |
R1659 |
T2914 |
T2910 |
ccomp |
are,contain |
R1660 |
T2915 |
T2914 |
prep |
of,are |
R1661 |
T2916 |
T2917 |
det |
a,length |
R1662 |
T2917 |
T2915 |
pobj |
length,of |
R1663 |
T2918 |
T2917 |
amod |
reasonable,length |
R1664 |
T2919 |
T2910 |
punct |
: ,contain |
R1665 |
T2920 |
T2921 |
mark |
although,determine |
R1666 |
T2921 |
T2910 |
advcl |
determine,contain |
R1667 |
T2922 |
T2921 |
nsubj |
we,determine |
R1668 |
T2923 |
T2921 |
aux |
did,determine |
R1669 |
T2924 |
T2921 |
neg |
not,determine |
R1670 |
T2925 |
T2926 |
mark |
whether,are |
R1671 |
T2926 |
T2921 |
ccomp |
are,determine |
R1672 |
T2927 |
T2926 |
nsubj |
clones,are |
R1673 |
T2928 |
T2929 |
amod |
full,length |
R1674 |
T2929 |
T2926 |
attr |
length,are |
R1675 |
T2930 |
T2929 |
punct |
-,length |
R1676 |
T2931 |
T2910 |
punct |
", ",contain |
R1677 |
T2932 |
T2933 |
quantmod |
881,"1,264" |
R1678 |
T2933 |
T2935 |
nummod |
"1,264",cDNAs |
R1679 |
T2934 |
T2933 |
quantmod |
of,"1,264" |
R1680 |
T2935 |
T2910 |
nsubj |
cDNAs,contain |
R1681 |
T2936 |
T2937 |
punct |
(,% |
R1682 |
T2937 |
T2933 |
parataxis |
%,"1,264" |
R1683 |
T2938 |
T2937 |
nummod |
70,% |
R1684 |
T2939 |
T2937 |
punct |
),% |
R1685 |
T2940 |
T2941 |
amod |
olfactory,receptor |
R1686 |
T2941 |
T2935 |
compound |
receptor,cDNAs |
R1687 |
T2942 |
T2943 |
det |
the,gene |
R1688 |
T2943 |
T2944 |
poss |
gene,codon |
R1689 |
T2944 |
T2910 |
dobj |
codon,contain |
R1690 |
T2945 |
T2943 |
case |
's,gene |
R1691 |
T2946 |
T2944 |
compound |
start,codon |
R1692 |
T2947 |
T2944 |
cc |
and,codon |
R1693 |
T2948 |
T2949 |
advmod |
at,least |
R1694 |
T2949 |
T2950 |
advmod |
least,sequence |
R1695 |
T2950 |
T2944 |
conj |
sequence,codon |
R1696 |
T2951 |
T2950 |
det |
some,sequence |
R1697 |
T2952 |
T2953 |
nummod |
5,UTR |
R1698 |
T2953 |
T2950 |
compound |
UTR,sequence |
R1699 |
T2954 |
T2952 |
punct |
',5 |
R1700 |
T2955 |
T2910 |
punct |
.,contain |
R1701 |
T2957 |
T2958 |
prep |
In,updated |
R1702 |
T2959 |
T2957 |
pobj |
order,In |
R1703 |
T2960 |
T2961 |
aux |
to,match |
R1704 |
T2961 |
T2959 |
acl |
match,order |
R1705 |
T2962 |
T2961 |
dobj |
cDNAs,match |
R1706 |
T2963 |
T2961 |
prep |
to,match |
R1707 |
T2964 |
T2965 |
poss |
their,counterparts |
R1708 |
T2965 |
T2963 |
pobj |
counterparts,to |
R1709 |
T2966 |
T2965 |
amod |
genomic,counterparts |
R1710 |
T2967 |
T2958 |
punct |
", ",updated |
R1711 |
T2968 |
T2958 |
advmod |
first,updated |
R1712 |
T2969 |
T2958 |
nsubj |
we,updated |
R1713 |
T2970 |
T2971 |
poss |
our,catalog |
R1714 |
T2971 |
T2958 |
dobj |
catalog,updated |
R1715 |
T2972 |
T2971 |
prep |
of,catalog |
R1716 |
T2973 |
T2974 |
nmod |
mouse,genes |
R1717 |
T2974 |
T2972 |
pobj |
genes,of |
R1718 |
T2975 |
T2976 |
amod |
olfactory,receptor |
R1719 |
T2976 |
T2974 |
compound |
receptor,genes |
R1720 |
T2977 |
T2978 |
punct |
[,1 |
R1721 |
T2978 |
T2971 |
parataxis |
1,catalog |
R1722 |
T2979 |
T2978 |
punct |
],1 |
R1723 |
T2980 |
T2958 |
prep |
based,updated |
R1724 |
T2981 |
T2980 |
prep |
on,based |
R1725 |
T2982 |
T2983 |
poss |
Celera,assembly |
R1726 |
T2983 |
T2981 |
pobj |
assembly,on |
R1727 |
T2984 |
T2982 |
case |
's,Celera |
R1728 |
T2985 |
T2986 |
advmod |
most,recent |
R1729 |
T2986 |
T2983 |
amod |
recent,assembly |
R1730 |
T2987 |
T2983 |
compound |
genome,assembly |
R1731 |
T2988 |
T2989 |
punct |
(,Release |
R1732 |
T2989 |
T2983 |
parataxis |
Release,assembly |
R1733 |
T2990 |
T2989 |
nummod |
13,Release |
R1734 |
T2991 |
T2989 |
punct |
),Release |
R1735 |
T2992 |
T2993 |
punct |
[,29 |
R1736 |
T2993 |
T2983 |
parataxis |
29,assembly |
R1737 |
T2994 |
T2993 |
punct |
],29 |
R1738 |
T2995 |
T2958 |
punct |
.,updated |
R1739 |
T2997 |
T2998 |
amod |
Previous,reports |
R1740 |
T2998 |
T2999 |
nsubjpass |
reports,based |
R1741 |
T3000 |
T2998 |
prep |
of,reports |
R1742 |
T3001 |
T3002 |
det |
the,repertoire |
R1743 |
T3002 |
T3000 |
pobj |
repertoire,of |
R1744 |
T3003 |
T3004 |
nmod |
mouse,receptor |
R1745 |
T3004 |
T3002 |
compound |
receptor,repertoire |
R1746 |
T3005 |
T3004 |
amod |
olfactory,receptor |
R1747 |
T3006 |
T3007 |
punct |
[,2 |
R1748 |
T3007 |
T2998 |
parataxis |
2,reports |
R1749 |
T3008 |
T3007 |
nummod |
1,2 |
R1750 |
T3009 |
T3007 |
punct |
",",2 |
R1751 |
T3010 |
T3007 |
punct |
],2 |
R1752 |
T3011 |
T2999 |
auxpass |
were,based |
R1753 |
T3012 |
T2999 |
prep |
on,based |
R1754 |
T3013 |
T3014 |
det |
the,assembly |
R1755 |
T3014 |
T3012 |
pobj |
assembly,on |
R1756 |
T3015 |
T3014 |
nmod |
Release,assembly |
R1757 |
T3016 |
T3015 |
nummod |
12,Release |
R1758 |
T3017 |
T2999 |
punct |
.,based |
R1759 |
T3019 |
T3020 |
nsubj |
Release,consists |
R1760 |
T3021 |
T3019 |
nummod |
13,Release |
R1761 |
T3022 |
T3020 |
prep |
of,consists |
R1762 |
T3023 |
T3024 |
amod |
fewer,longer |
R1763 |
T3024 |
T3026 |
amod |
longer,sequences |
R1764 |
T3025 |
T3024 |
punct |
", ",longer |
R1765 |
T3026 |
T3022 |
pobj |
sequences,of |
R1766 |
T3027 |
T3026 |
compound |
scaffold,sequences |
R1767 |
T3028 |
T3026 |
acl |
containing,sequences |
R1768 |
T3029 |
T3030 |
amod |
fewer,smaller |
R1769 |
T3030 |
T3032 |
amod |
smaller,gaps |
R1770 |
T3031 |
T3030 |
punct |
", ",smaller |
R1771 |
T3032 |
T3028 |
dobj |
gaps,containing |
R1772 |
T3033 |
T3032 |
prep |
than,gaps |
R1773 |
T3034 |
T3033 |
pobj |
Release,than |
R1774 |
T3035 |
T3034 |
nummod |
12,Release |
R1775 |
T3036 |
T3020 |
punct |
.,consists |
R1776 |
T3038 |
T3039 |
advcl |
Using,identified |
R1777 |
T3040 |
T3041 |
det |
the,methods |
R1778 |
T3041 |
T3038 |
dobj |
methods,Using |
R1779 |
T3042 |
T3043 |
npadvmod |
BLAST,based |
R1780 |
T3043 |
T3041 |
amod |
based,methods |
R1781 |
T3044 |
T3043 |
punct |
-,based |
R1782 |
T3045 |
T3041 |
acl |
detailed,methods |
R1783 |
T3046 |
T3045 |
advmod |
previously,detailed |
R1784 |
T3047 |
T3048 |
punct |
[,1 |
R1785 |
T3048 |
T3045 |
parataxis |
1,detailed |
R1786 |
T3049 |
T3048 |
punct |
],1 |
R1787 |
T3050 |
T3039 |
punct |
", ",identified |
R1788 |
T3051 |
T3039 |
nsubj |
we,identified |
R1789 |
T3052 |
T3053 |
nummod |
"1,490",sequences |
R1790 |
T3053 |
T3039 |
dobj |
sequences,identified |
R1791 |
T3054 |
T3055 |
amod |
olfactory,receptor |
R1792 |
T3055 |
T3053 |
compound |
receptor,sequences |
R1793 |
T3056 |
T3039 |
prep |
in,identified |
R1794 |
T3057 |
T3058 |
det |
the,assembly |
R1795 |
T3058 |
T3056 |
pobj |
assembly,in |
R1796 |
T3059 |
T3058 |
amod |
new,assembly |
R1797 |
T3060 |
T3039 |
punct |
", ",identified |
R1798 |
T3061 |
T3039 |
prep |
including,identified |
R1799 |
T3062 |
T3063 |
nummod |
"1,107",genes |
R1800 |
T3063 |
T3061 |
pobj |
genes,including |
R1801 |
T3064 |
T3063 |
amod |
intact,genes |
R1802 |
T3065 |
T3066 |
amod |
olfactory,receptor |
R1803 |
T3066 |
T3063 |
compound |
receptor,genes |
R1804 |
T3067 |
T3039 |
punct |
(,identified |
R1805 |
T3068 |
T3039 |
prep |
compared,identified |
R1806 |
T3069 |
T3068 |
prep |
to,compared |
R1807 |
T3070 |
T3071 |
nummod |
866,receptors |
R1808 |
T3071 |
T3069 |
pobj |
receptors,to |
R1809 |
T3072 |
T3071 |
amod |
intact,receptors |
R1810 |
T3073 |
T3071 |
amod |
olfactory,receptors |
R1811 |
T3074 |
T3071 |
prep |
in,receptors |
R1812 |
T3075 |
T3076 |
det |
the,assembly |
R1813 |
T3076 |
T3074 |
pobj |
assembly,in |
R1814 |
T3077 |
T3076 |
amod |
old,assembly |
R1815 |
T3078 |
T3039 |
punct |
),identified |
R1816 |
T3079 |
T3039 |
advcl |
reflecting,identified |
R1817 |
T3080 |
T3081 |
det |
the,rate |
R1818 |
T3081 |
T3079 |
dobj |
rate,reflecting |
R1819 |
T3082 |
T3081 |
amod |
reduced,rate |
R1820 |
T3083 |
T3084 |
compound |
sequence,error |
R1821 |
T3084 |
T3081 |
compound |
error,rate |
R1822 |
T3085 |
T3081 |
cc |
and,rate |
R1823 |
T3086 |
T3087 |
amod |
increased,coverage |
R1824 |
T3087 |
T3081 |
conj |
coverage,rate |
R1825 |
T3088 |
T3087 |
prep |
of,coverage |
R1826 |
T3089 |
T3090 |
det |
the,assembly |
R1827 |
T3090 |
T3088 |
pobj |
assembly,of |
R1828 |
T3091 |
T3090 |
amod |
new,assembly |
R1829 |
T3092 |
T3093 |
punct |
(,Table |
R1830 |
T3093 |
T3079 |
parataxis |
Table,reflecting |
R1831 |
T3094 |
T3093 |
nummod |
1,Table |
R1832 |
T3095 |
T3093 |
punct |
),Table |
R1833 |
T3096 |
T3039 |
punct |
.,identified |
R1834 |
T3098 |
T3099 |
nsubj |
We,created |
R1835 |
T3100 |
T3101 |
det |
a,database |
R1836 |
T3101 |
T3099 |
dobj |
database,created |
R1837 |
T3102 |
T3101 |
amod |
local,database |
R1838 |
T3103 |
T3101 |
prep |
of,database |
R1839 |
T3104 |
T3105 |
amod |
genomic,sequences |
R1840 |
T3105 |
T3103 |
pobj |
sequences,of |
R1841 |
T3106 |
T3101 |
prep |
including,database |
R1842 |
T3107 |
T3108 |
det |
all,loci |
R1843 |
T3108 |
T3106 |
pobj |
loci,including |
R1844 |
T3109 |
T3110 |
amod |
olfactory,receptor |
R1845 |
T3110 |
T3108 |
compound |
receptor,loci |
R1846 |
T3111 |
T3108 |
cc |
and,loci |
R1847 |
T3112 |
T3113 |
nummod |
0.5,Mb |
R1848 |
T3113 |
T3114 |
compound |
Mb,sequences |
R1849 |
T3114 |
T3108 |
conj |
sequences,loci |
R1850 |
T3115 |
T3114 |
compound |
flanking,sequences |
R1851 |
T3116 |
T3117 |
punct |
(,available |
R1852 |
T3117 |
T3114 |
parataxis |
available,sequences |
R1853 |
T3118 |
T3117 |
mark |
if,available |
R1854 |
T3119 |
T3117 |
punct |
),available |
R1855 |
T3120 |
T3099 |
cc |
and,created |
R1856 |
T3121 |
T3099 |
conj |
compared,created |
R1857 |
T3122 |
T3123 |
det |
each,sequence |
R1858 |
T3123 |
T3121 |
dobj |
sequence,compared |
R1859 |
T3124 |
T3123 |
compound |
cDNA,sequence |
R1860 |
T3125 |
T3121 |
prep |
to,compared |
R1861 |
T3126 |
T3127 |
det |
this,database |
R1862 |
T3127 |
T3125 |
pobj |
database,to |
R1863 |
T3128 |
T3127 |
punct |
',database |
R1864 |
T3129 |
T3130 |
amod |
olfactory,subgenome |
R1865 |
T3130 |
T3127 |
nmod |
subgenome,database |
R1866 |
T3131 |
T3127 |
punct |
',database |
R1867 |
T3132 |
T3121 |
advcl |
using,compared |
R1868 |
T3133 |
T3132 |
dobj |
sim4,using |
R1869 |
T3134 |
T3135 |
punct |
[,30 |
R1870 |
T3135 |
T3121 |
parataxis |
30,compared |
R1871 |
T3136 |
T3135 |
punct |
],30 |
R1872 |
T3137 |
T3099 |
punct |
.,created |
R1873 |
T3139 |
T3140 |
nsubjpass |
cDNAs,assigned |
R1874 |
T3141 |
T3140 |
auxpass |
were,assigned |
R1875 |
T3142 |
T3140 |
prep |
to,assigned |
R1876 |
T3143 |
T3144 |
amod |
individual,genes |
R1877 |
T3144 |
T3142 |
pobj |
genes,to |
R1878 |
T3145 |
T3140 |
prep |
based,assigned |
R1879 |
T3146 |
T3145 |
prep |
on,based |
R1880 |
T3147 |
T3148 |
poss |
their,match |
R1881 |
T3148 |
T3146 |
pobj |
match,on |
R1882 |
T3149 |
T3148 |
amod |
best,match |
R1883 |
T3150 |
T3148 |
prep |
to,match |
R1884 |
T3151 |
T3152 |
det |
an,region |
R1885 |
T3152 |
T3150 |
pobj |
region,to |
R1886 |
T3153 |
T3154 |
amod |
olfactory,receptor |
R1887 |
T3154 |
T3152 |
compound |
receptor,region |
R1888 |
T3155 |
T3152 |
compound |
coding,region |
R1889 |
T3156 |
T3152 |
cc |
or,region |
R1890 |
T3157 |
T3158 |
poss |
its,region |
R1891 |
T3158 |
T3152 |
conj |
region,region |
R1892 |
T3159 |
T3158 |
amod |
upstream,region |
R1893 |
T3160 |
T3161 |
punct |
(,see |
R1894 |
T3161 |
T3140 |
parataxis |
see,assigned |
R1895 |
T3162 |
T3161 |
dobj |
Materials,see |
R1896 |
T3163 |
T3162 |
cc |
and,Materials |
R1897 |
T3164 |
T3162 |
conj |
methods,Materials |
R1898 |
T3165 |
T3161 |
punct |
),see |
R1899 |
T3166 |
T3140 |
punct |
.,assigned |
R1900 |
T3168 |
T3169 |
prep |
Of,matched |
R1901 |
T3169 |
T3177 |
ccomp |
matched,matched |
R1902 |
T3170 |
T3171 |
det |
the,cDNAs |
R1903 |
T3171 |
T3168 |
pobj |
cDNAs,Of |
R1904 |
T3172 |
T3171 |
nummod |
"1,264",cDNAs |
R1905 |
T3173 |
T3174 |
amod |
olfactory,receptor |
R1906 |
T3174 |
T3171 |
compound |
receptor,cDNAs |
R1907 |
T3175 |
T3169 |
punct |
", ",matched |
R1908 |
T3176 |
T3169 |
nsubj |
"1,176",matched |
R1909 |
T3178 |
T3179 |
det |
a,total |
R1910 |
T3179 |
T3169 |
dobj |
total,matched |
R1911 |
T3180 |
T3179 |
prep |
of,total |
R1912 |
T3181 |
T3182 |
nummod |
419,genes |
R1913 |
T3182 |
T3180 |
pobj |
genes,of |
R1914 |
T3183 |
T3184 |
amod |
olfactory,receptor |
R1915 |
T3184 |
T3182 |
compound |
receptor,genes |
R1916 |
T3185 |
T3177 |
punct |
;,matched |
R1917 |
T3186 |
T3187 |
det |
the,cDNAs |
R1918 |
T3187 |
T3177 |
nsubj |
cDNAs,matched |
R1919 |
T3188 |
T3187 |
amod |
remaining,cDNAs |
R1920 |
T3189 |
T3177 |
preconj |
either,matched |
R1921 |
T3190 |
T3191 |
det |
an,receptor |
R1922 |
T3191 |
T3177 |
dobj |
receptor,matched |
R1923 |
T3192 |
T3191 |
amod |
olfactory,receptor |
R1924 |
T3193 |
T3177 |
prep |
below,matched |
R1925 |
T3194 |
T3195 |
poss |
our,threshold |
R1926 |
T3195 |
T3193 |
pobj |
threshold,below |
R1927 |
T3196 |
T3197 |
nummod |
96,% |
R1928 |
T3197 |
T3198 |
compound |
%,identity |
R1929 |
T3198 |
T3195 |
compound |
identity,threshold |
R1930 |
T3199 |
T3198 |
compound |
nucleotide,identity |
R1931 |
T3200 |
T3177 |
cc |
or,matched |
R1932 |
T3201 |
T3177 |
conj |
had,matched |
R1933 |
T3202 |
T3203 |
amod |
ambiguous,matches |
R1934 |
T3203 |
T3201 |
dobj |
matches,had |
R1935 |
T3204 |
T3203 |
acl |
encompassing,matches |
R1936 |
T3205 |
T3206 |
amod |
more,one |
R1937 |
T3206 |
T3208 |
nummod |
one,region |
R1938 |
T3207 |
T3206 |
quantmod |
than,one |
R1939 |
T3208 |
T3204 |
dobj |
region,encompassing |
R1940 |
T3209 |
T3210 |
amod |
olfactory,receptor |
R1941 |
T3210 |
T3211 |
compound |
receptor,gene |
R1942 |
T3211 |
T3208 |
compound |
gene,region |
R1943 |
T3212 |
T3213 |
punct |
(,see |
R1944 |
T3213 |
T3201 |
parataxis |
see,had |
R1945 |
T3214 |
T3213 |
advmod |
below,see |
R1946 |
T3215 |
T3213 |
punct |
),see |
R1947 |
T3216 |
T3177 |
punct |
.,matched |