craft-sa-dev Home  

PMC:329117 / 45060-47408 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T12269 0-2 NN denotes RT
T12271 2-3 HYPH denotes -
T12270 3-6 NN denotes PCR
T12272 6-135 sentence denotes The olfactory epithelia were dissected from three adult female C57BL/6 mice, including tissues attached to the skull and septum.
T12273 7-10 DT denotes The
T12275 11-20 JJ denotes olfactory
T12274 21-30 NNS denotes epithelia
T12277 31-35 VBD denotes were
T12276 36-45 VBN denotes dissected
T12278 46-50 IN denotes from
T12279 51-56 CD denotes three
T12281 57-62 JJ denotes adult
T12282 63-69 JJ denotes female
T12283 70-75 NN denotes C57BL
T12284 75-76 HYPH denotes /
T12285 76-77 CD denotes 6
T12280 78-82 NNS denotes mice
T12286 82-84 , denotes ,
T12287 84-93 VBG denotes including
T12288 94-101 NNS denotes tissues
T12289 102-110 VBN denotes attached
T12290 111-113 IN denotes to
T12291 114-117 DT denotes the
T12292 118-123 NN denotes skull
T12293 124-127 CC denotes and
T12294 128-134 NN denotes septum
T12295 134-135 . denotes .
T12296 135-248 sentence denotes RNA was isolated using the Qiagen RNeasy midi kit (Qiagen, Valencia, CA, USA), including a DNase treatment step.
T12297 136-139 NN denotes RNA
T12299 140-143 VBD denotes was
T12298 144-152 VBN denotes isolated
T12300 153-158 VBG denotes using
T12301 159-162 DT denotes the
T12303 163-169 NNP denotes Qiagen
T12304 170-176 NNP denotes RNeasy
T12305 177-181 NN denotes midi
T12302 182-185 NN denotes kit
T12306 186-187 -LRB- denotes (
T12307 187-193 NNP denotes Qiagen
T12308 193-195 , denotes ,
T12309 195-203 NNP denotes Valencia
T12310 203-205 , denotes ,
T12311 205-207 NNP denotes CA
T12312 207-209 , denotes ,
T12313 209-212 NNP denotes USA
T12314 212-213 -RRB- denotes )
T12315 213-215 , denotes ,
T12316 215-224 VBG denotes including
T12317 225-226 DT denotes a
T12319 227-232 NN denotes DNase
T12320 233-242 NN denotes treatment
T12318 243-247 NN denotes step
T12321 247-248 . denotes .
T12322 248-477 sentence denotes First-strand cDNA was produced from 2.5 μg of RNA in a volume of 50 μl using random hexamers and Invitrogen's Superscript II reverse transcriptase (Invitrogen, Carlsbad, CA, USA), according to the manufacturer's recommendations.
T12323 249-254 JJ denotes First
T12325 254-255 HYPH denotes -
T12324 255-261 NN denotes strand
T12326 262-266 NN denotes cDNA
T12328 267-270 VBD denotes was
T12327 271-279 VBN denotes produced
T12329 280-284 IN denotes from
T12330 285-288 CD denotes 2.5
T12331 289-291 NNS denotes μg
T12332 292-294 IN denotes of
T12333 295-298 NN denotes RNA
T12334 299-301 IN denotes in
T12335 302-303 DT denotes a
T12336 304-310 NN denotes volume
T12337 311-313 IN denotes of
T12338 314-316 CD denotes 50
T12339 317-319 NNS denotes μl
T12340 320-325 VBG denotes using
T12341 326-332 JJ denotes random
T12342 333-341 NNS denotes hexamers
T12343 342-345 CC denotes and
T12344 346-356 NNP denotes Invitrogen
T12346 356-358 POS denotes 's
T12347 359-370 NN denotes Superscript
T12348 371-373 CD denotes II
T12349 374-381 JJ denotes reverse
T12345 382-395 NN denotes transcriptase
T12350 396-397 -LRB- denotes (
T12351 397-407 NNP denotes Invitrogen
T12352 407-409 , denotes ,
T12353 409-417 NNP denotes Carlsbad
T12354 417-419 , denotes ,
T12355 419-421 NNP denotes CA
T12356 421-423 , denotes ,
T12357 423-426 NNP denotes USA
T12358 426-427 -RRB- denotes )
T12359 427-429 , denotes ,
T12360 429-438 VBG denotes according
T12361 439-441 IN denotes to
T12362 442-445 DT denotes the
T12363 446-458 NN denotes manufacturer
T12365 458-460 POS denotes 's
T12364 461-476 NNS denotes recommendations
T12366 476-477 . denotes .
T12367 477-566 sentence denotes One-twenty-fifth of the resulting cDNA was used as template in subsequent PCR reactions.
T12368 478-481 CD denotes One
T12370 481-482 HYPH denotes -
T12371 482-488 CD denotes twenty
T12372 488-489 HYPH denotes -
T12369 489-494 NN denotes fifth
T12374 495-497 IN denotes of
T12375 498-501 DT denotes the
T12377 502-511 VBG denotes resulting
T12376 512-516 NN denotes cDNA
T12378 517-520 VBD denotes was
T12373 521-525 VBN denotes used
T12379 526-528 IN denotes as
T12380 529-537 NN denotes template
T12381 538-540 IN denotes in
T12382 541-551 JJ denotes subsequent
T12384 552-555 NN denotes PCR
T12383 556-565 NNS denotes reactions
T12385 565-566 . denotes .
T12386 566-754 sentence denotes PCR amplification biased towards class I olfactory receptors was performed using degenerate primers P26 [17] and classI_R1 (5'-GGRTTIADIRYIGGNGG-3') with an annealing temperature of 44°C.
T12387 567-570 NN denotes PCR
T12388 571-584 NN denotes amplification
T12390 585-591 JJ denotes biased
T12391 592-599 IN denotes towards
T12392 600-605 NN denotes class
T12394 606-607 CD denotes I
T12395 608-617 JJ denotes olfactory
T12393 618-627 NNS denotes receptors
T12396 628-631 VBD denotes was
T12389 632-641 VBN denotes performed
T12397 642-647 VBG denotes using
T12398 648-658 JJ denotes degenerate
T12400 659-666 NNS denotes primers
T12399 667-670 NN denotes P26
T12401 671-672 -LRB- denotes [
T12402 672-674 CD denotes 17
T12403 674-675 -RRB- denotes ]
T12404 676-679 CC denotes and
T12405 680-689 NN denotes classI_R1
T12406 690-691 -LRB- denotes (
T12408 691-692 CD denotes 5
T12409 692-693 SYM denotes '
T12410 693-694 HYPH denotes -
T12407 694-711 NN denotes GGRTTIADIRYIGGNGG
T12411 711-712 HYPH denotes -
T12412 712-713 CD denotes 3
T12413 713-714 SYM denotes '
T12414 714-715 -RRB- denotes )
T12415 716-720 IN denotes with
T12416 721-723 DT denotes an
T12418 724-733 JJ denotes annealing
T12417 734-745 NN denotes temperature
T12419 746-748 IN denotes of
T12420 749-751 CD denotes 44
T12421 751-753 NNS denotes °C
T12422 753-754 . denotes .
T12423 754-845 sentence denotes The product was cloned (TA cloning kit, Invitrogen), and individual clones were sequenced.
T12424 755-758 DT denotes The
T12425 759-766 NN denotes product
T12427 767-770 VBD denotes was
T12426 771-777 VBN denotes cloned
T12428 778-779 -LRB- denotes (
T12430 779-781 NN denotes TA
T12432 782-789 NN denotes cloning
T12431 790-793 NN denotes kit
T12433 793-795 , denotes ,
T12429 795-805 NNP denotes Invitrogen
T12434 805-806 -RRB- denotes )
T12435 806-808 , denotes ,
T12436 808-811 CC denotes and
T12437 812-822 JJ denotes individual
T12438 823-829 NNS denotes clones
T12440 830-834 VBD denotes were
T12439 835-844 VBN denotes sequenced
T12441 844-845 . denotes .
T12442 845-969 sentence denotes Specific PCR primers used to confirm expression of individual olfactory receptor genes are given in Additional data file 1.
T12443 846-854 JJ denotes Specific
T12445 855-858 NN denotes PCR
T12444 859-866 NNS denotes primers
T12447 867-871 VBN denotes used
T12448 872-874 TO denotes to
T12449 875-882 VB denotes confirm
T12450 883-893 NN denotes expression
T12451 894-896 IN denotes of
T12452 897-907 JJ denotes individual
T12454 908-917 JJ denotes olfactory
T12455 918-926 NN denotes receptor
T12453 927-932 NNS denotes genes
T12456 933-936 VBP denotes are
T12446 937-942 VBN denotes given
T12457 943-945 IN denotes in
T12458 946-956 JJ denotes Additional
T12460 957-961 NNS denotes data
T12459 962-966 NN denotes file
T12461 967-968 CD denotes 1
T12462 968-969 . denotes .
T12463 969-1068 sentence denotes Each PCR product was sequenced to confirm that the expected gene and no others had been amplified.
T12464 970-974 DT denotes Each
T12466 975-978 NN denotes PCR
T12465 979-986 NN denotes product
T12468 987-990 VBD denotes was
T12467 991-1000 VBN denotes sequenced
T12469 1001-1003 TO denotes to
T12470 1004-1011 VB denotes confirm
T12471 1012-1016 IN denotes that
T12473 1017-1020 DT denotes the
T12475 1021-1029 VBN denotes expected
T12474 1030-1034 NN denotes gene
T12476 1035-1038 CC denotes and
T12477 1039-1041 DT denotes no
T12478 1042-1048 NNS denotes others
T12479 1049-1052 VBD denotes had
T12480 1053-1057 VBN denotes been
T12472 1058-1067 VBN denotes amplified
T12481 1067-1068 . denotes .
T12482 1068-1227 sentence denotes Control reactions on a template made by omitting reverse transcriptase gave no product, indicating that the RNA preparation was uncontaminated by genomic DNA.
T12483 1069-1076 NN denotes Control
T12484 1077-1086 NNS denotes reactions
T12486 1087-1089 IN denotes on
T12487 1090-1091 DT denotes a
T12488 1092-1100 NN denotes template
T12489 1101-1105 VBN denotes made
T12490 1106-1108 IN denotes by
T12491 1109-1117 VBG denotes omitting
T12492 1118-1125 JJ denotes reverse
T12493 1126-1139 NN denotes transcriptase
T12485 1140-1144 VBD denotes gave
T12494 1145-1147 DT denotes no
T12495 1148-1155 NN denotes product
T12496 1155-1157 , denotes ,
T12497 1157-1167 VBG denotes indicating
T12498 1168-1172 IN denotes that
T12500 1173-1176 DT denotes the
T12502 1177-1180 NN denotes RNA
T12501 1181-1192 NN denotes preparation
T12499 1193-1196 VBD denotes was
T12503 1197-1211 JJ denotes uncontaminated
T12504 1212-1214 IN denotes by
T12505 1215-1222 JJ denotes genomic
T12506 1223-1226 NN denotes DNA
T12507 1226-1227 . denotes .
T12508 1227-1430 sentence denotes Relative transcript levels were estimated using real-time PCR according to Applied Biosystems' protocols, with magnesium concentration, primer pair and fluorescent probe given in Additional data file 2.
T12509 1228-1236 JJ denotes Relative
T12511 1237-1247 NN denotes transcript
T12510 1248-1254 NNS denotes levels
T12513 1255-1259 VBD denotes were
T12512 1260-1269 VBN denotes estimated
T12514 1270-1275 VBG denotes using
T12515 1276-1280 JJ denotes real
T12517 1280-1281 HYPH denotes -
T12516 1281-1285 NN denotes time
T12518 1286-1289 NN denotes PCR
T12519 1290-1299 VBG denotes according
T12520 1300-1302 IN denotes to
T12521 1303-1310 NNP denotes Applied
T12522 1311-1321 NNP denotes Biosystems
T12524 1321-1322 POS denotes '
T12523 1323-1332 NNS denotes protocols
T12525 1332-1334 , denotes ,
T12526 1334-1338 IN denotes with
T12528 1339-1348 NN denotes magnesium
T12529 1349-1362 NN denotes concentration
T12530 1362-1364 , denotes ,
T12531 1364-1370 NN denotes primer
T12532 1371-1375 NN denotes pair
T12533 1376-1379 CC denotes and
T12534 1380-1391 JJ denotes fluorescent
T12535 1392-1397 NN denotes probe
T12527 1398-1403 VBN denotes given
T12536 1404-1406 IN denotes in
T12537 1407-1417 JJ denotes Additional
T12539 1418-1422 NNS denotes data
T12538 1423-1427 NN denotes file
T12540 1428-1429 CD denotes 2
T12541 1429-1430 . denotes .
T12542 1430-1517 sentence denotes The increase in fluorescence during thermocycling was measured on an ABI PRISM 7900HT.
T12543 1431-1434 DT denotes The
T12544 1435-1443 NN denotes increase
T12546 1444-1446 IN denotes in
T12547 1447-1459 NN denotes fluorescence
T12548 1460-1466 IN denotes during
T12549 1467-1480 NN denotes thermocycling
T12550 1481-1484 VBD denotes was
T12545 1485-1493 VBN denotes measured
T12551 1494-1496 IN denotes on
T12552 1497-1499 DT denotes an
T12554 1500-1503 NN denotes ABI
T12555 1504-1509 NN denotes PRISM
T12553 1510-1516 NN denotes 7900HT
T12556 1516-1517 . denotes .
T12557 1517-1715 sentence denotes Standard curves were constructed for each primer pair using triplicate samples of mouse genomic DNA of nine known concentrations (range 0.01-100 ng, or about 3-30,000 copies of the haploid genome).
T12558 1518-1526 JJ denotes Standard
T12559 1527-1533 NNS denotes curves
T12561 1534-1538 VBD denotes were
T12560 1539-1550 VBN denotes constructed
T12562 1551-1554 IN denotes for
T12563 1555-1559 DT denotes each
T12565 1560-1566 NN denotes primer
T12564 1567-1571 NN denotes pair
T12566 1572-1577 VBG denotes using
T12567 1578-1588 NN denotes triplicate
T12568 1589-1596 NNS denotes samples
T12569 1597-1599 IN denotes of
T12570 1600-1605 NN denotes mouse
T12572 1606-1613 JJ denotes genomic
T12571 1614-1617 NN denotes DNA
T12573 1618-1620 IN denotes of
T12574 1621-1625 CD denotes nine
T12576 1626-1631 VBN denotes known
T12575 1632-1646 NNS denotes concentrations
T12577 1647-1648 -LRB- denotes (
T12579 1648-1653 NN denotes range
T12580 1654-1658 CD denotes 0.01
T12582 1658-1659 SYM denotes -
T12581 1659-1662 CD denotes 100
T12578 1663-1665 NNS denotes ng
T12583 1665-1667 , denotes ,
T12584 1667-1669 CC denotes or
T12585 1670-1675 RB denotes about
T12587 1676-1677 CD denotes 3
T12588 1677-1678 SYM denotes -
T12586 1678-1684 CD denotes 30,000
T12589 1685-1691 NNS denotes copies
T12590 1692-1694 IN denotes of
T12591 1695-1698 DT denotes the
T12593 1699-1706 NN denotes haploid
T12592 1707-1713 NN denotes genome
T12594 1713-1714 -RRB- denotes )
T12595 1714-1715 . denotes .
T12596 1715-1974 sentence denotes Relative expression level of each gene was determined by comparing the mean Ct (cycle where fluorescence reaches an arbitrary threshold value) obtained with six replicate samples of reverse-transcribed RNA to the standard curve for the corresponding primers.
T12597 1716-1724 JJ denotes Relative
T12599 1725-1735 NN denotes expression
T12598 1736-1741 NN denotes level
T12601 1742-1744 IN denotes of
T12602 1745-1749 DT denotes each
T12603 1750-1754 NN denotes gene
T12604 1755-1758 VBD denotes was
T12600 1759-1769 VBN denotes determined
T12605 1770-1772 IN denotes by
T12606 1773-1782 VBG denotes comparing
T12607 1783-1786 DT denotes the
T12609 1787-1791 JJ denotes mean
T12608 1792-1794 NN denotes Ct
T12610 1795-1796 -LRB- denotes (
T12611 1796-1801 NN denotes cycle
T12612 1802-1807 WRB denotes where
T12614 1808-1820 NN denotes fluorescence
T12613 1821-1828 VBZ denotes reaches
T12615 1829-1831 DT denotes an
T12617 1832-1841 JJ denotes arbitrary
T12618 1842-1851 NN denotes threshold
T12616 1852-1857 NN denotes value
T12619 1857-1858 -RRB- denotes )
T12620 1859-1867 VBN denotes obtained
T12621 1868-1872 IN denotes with
T12622 1873-1876 CD denotes six
T12624 1877-1886 NN denotes replicate
T12623 1887-1894 NNS denotes samples
T12625 1895-1897 IN denotes of
T12626 1898-1905 JJ denotes reverse
T12628 1905-1906 HYPH denotes -
T12627 1906-1917 JJ denotes transcribed
T12629 1918-1921 NN denotes RNA
T12630 1922-1924 IN denotes to
T12631 1925-1928 DT denotes the
T12633 1929-1937 JJ denotes standard
T12632 1938-1943 NN denotes curve
T12634 1944-1947 IN denotes for
T12635 1948-1951 DT denotes the
T12637 1952-1965 VBG denotes corresponding
T12636 1966-1973 NNS denotes primers
T12638 1973-1974 . denotes .
T12639 1974-2077 sentence denotes Relative RNA levels of a housekeeping gene, ribosomal S16, were measured as previously described [52].
T12640 1975-1983 JJ denotes Relative
T12642 1984-1987 NN denotes RNA
T12641 1988-1994 NNS denotes levels
T12644 1995-1997 IN denotes of
T12645 1998-1999 DT denotes a
T12647 2000-2012 NN denotes housekeeping
T12646 2013-2017 NN denotes gene
T12648 2017-2019 , denotes ,
T12649 2019-2028 JJ denotes ribosomal
T12650 2029-2032 NN denotes S16
T12651 2032-2034 , denotes ,
T12652 2034-2038 VBD denotes were
T12643 2039-2047 VBN denotes measured
T12653 2048-2050 IN denotes as
T12655 2051-2061 RB denotes previously
T12654 2062-2071 VBN denotes described
T12656 2072-2073 -LRB- denotes [
T12657 2073-2075 CD denotes 52
T12658 2075-2076 -RRB- denotes ]
T12659 2076-2077 . denotes .
T12660 2077-2220 sentence denotes Control reactions on template prepared by omitting reverse transcriptase amplified at a relative level of 0.03 ± 0.01 ng or less in each case.
T12661 2078-2085 NN denotes Control
T12662 2086-2095 NNS denotes reactions
T12664 2096-2098 IN denotes on
T12665 2099-2107 NN denotes template
T12666 2108-2116 VBN denotes prepared
T12667 2117-2119 IN denotes by
T12668 2120-2128 VBG denotes omitting
T12669 2129-2136 JJ denotes reverse
T12670 2137-2150 NN denotes transcriptase
T12663 2151-2160 VBN denotes amplified
T12671 2161-2163 IN denotes at
T12672 2164-2165 DT denotes a
T12674 2166-2174 JJ denotes relative
T12673 2175-2180 NN denotes level
T12675 2181-2183 IN denotes of
T12676 2184-2188 CD denotes 0.03
T12678 2189-2190 SYM denotes ±
T12677 2191-2195 CD denotes 0.01
T12679 2196-2198 NNS denotes ng
T12680 2199-2201 CC denotes or
T12681 2202-2206 JJR denotes less
T12682 2207-2209 IN denotes in
T12683 2210-2214 DT denotes each
T12684 2215-2219 NN denotes case
T12685 2219-2220 . denotes .
T12686 2220-2348 sentence denotes Expression measurements of the seven genes were normalized for each mouse so that S16 levels were equal to 1 (arbitrary units).
T12687 2221-2231 NN denotes Expression
T12688 2232-2244 NNS denotes measurements
T12690 2245-2247 IN denotes of
T12691 2248-2251 DT denotes the
T12693 2252-2257 CD denotes seven
T12692 2258-2263 NNS denotes genes
T12694 2264-2268 VBD denotes were
T12689 2269-2279 VBN denotes normalized
T12695 2280-2283 IN denotes for
T12696 2284-2288 DT denotes each
T12697 2289-2294 NN denotes mouse
T12698 2295-2297 IN denotes so
T12700 2298-2302 IN denotes that
T12701 2303-2306 NN denotes S16
T12702 2307-2313 NNS denotes levels
T12699 2314-2318 VBD denotes were
T12703 2319-2324 JJ denotes equal
T12704 2325-2327 IN denotes to
T12705 2328-2329 CD denotes 1
T12706 2330-2331 -LRB- denotes (
T12708 2331-2340 JJ denotes arbitrary
T12707 2341-2346 NNS denotes units
T12709 2346-2347 -RRB- denotes )
T12710 2347-2348 . denotes .
R7750 T12269 T12270 compound RT,PCR
R7751 T12271 T12270 punct -,PCR
R7752 T12273 T12274 det The,epithelia
R7753 T12274 T12276 nsubjpass epithelia,dissected
R7754 T12275 T12274 amod olfactory,epithelia
R7755 T12277 T12276 auxpass were,dissected
R7756 T12278 T12276 prep from,dissected
R7757 T12279 T12280 nummod three,mice
R7758 T12280 T12278 pobj mice,from
R7759 T12281 T12280 amod adult,mice
R7760 T12282 T12280 amod female,mice
R7761 T12283 T12280 nmod C57BL,mice
R7762 T12284 T12283 punct /,C57BL
R7763 T12285 T12283 nummod 6,C57BL
R7764 T12286 T12276 punct ", ",dissected
R7765 T12287 T12276 prep including,dissected
R7766 T12288 T12287 pobj tissues,including
R7767 T12289 T12288 acl attached,tissues
R7768 T12290 T12289 prep to,attached
R7769 T12291 T12292 det the,skull
R7770 T12292 T12290 pobj skull,to
R7771 T12293 T12292 cc and,skull
R7772 T12294 T12292 conj septum,skull
R7773 T12295 T12276 punct .,dissected
R7774 T12297 T12298 nsubjpass RNA,isolated
R7775 T12299 T12298 auxpass was,isolated
R7776 T12300 T12298 advcl using,isolated
R7777 T12301 T12302 det the,kit
R7778 T12302 T12300 dobj kit,using
R7779 T12303 T12302 compound Qiagen,kit
R7780 T12304 T12302 compound RNeasy,kit
R7781 T12305 T12302 compound midi,kit
R7782 T12306 T12307 punct (,Qiagen
R7783 T12307 T12302 parataxis Qiagen,kit
R7784 T12308 T12307 punct ", ",Qiagen
R7785 T12309 T12307 npadvmod Valencia,Qiagen
R7786 T12310 T12307 punct ", ",Qiagen
R7787 T12311 T12307 npadvmod CA,Qiagen
R7788 T12312 T12307 punct ", ",Qiagen
R7789 T12313 T12307 npadvmod USA,Qiagen
R7790 T12314 T12307 punct ),Qiagen
R7791 T12315 T12300 punct ", ",using
R7792 T12316 T12300 prep including,using
R7793 T12317 T12318 det a,step
R7794 T12318 T12316 pobj step,including
R7795 T12319 T12318 compound DNase,step
R7796 T12320 T12318 compound treatment,step
R7797 T12321 T12298 punct .,isolated
R7798 T12323 T12324 amod First,strand
R7799 T12324 T12326 compound strand,cDNA
R7800 T12325 T12324 punct -,strand
R7801 T12326 T12327 nsubjpass cDNA,produced
R7802 T12328 T12327 auxpass was,produced
R7803 T12329 T12327 prep from,produced
R7804 T12330 T12331 nummod 2.5,μg
R7805 T12331 T12329 pobj μg,from
R7806 T12332 T12331 prep of,μg
R7807 T12333 T12332 pobj RNA,of
R7808 T12334 T12331 prep in,μg
R7809 T12335 T12336 det a,volume
R7810 T12336 T12334 pobj volume,in
R7811 T12337 T12336 prep of,volume
R7812 T12338 T12339 nummod 50,μl
R7813 T12339 T12337 pobj μl,of
R7814 T12340 T12327 advcl using,produced
R7815 T12341 T12342 amod random,hexamers
R7816 T12342 T12340 dobj hexamers,using
R7817 T12343 T12342 cc and,hexamers
R7818 T12344 T12345 poss Invitrogen,transcriptase
R7819 T12345 T12342 conj transcriptase,hexamers
R7820 T12346 T12344 case 's,Invitrogen
R7821 T12347 T12345 nmod Superscript,transcriptase
R7822 T12348 T12347 nummod II,Superscript
R7823 T12349 T12345 amod reverse,transcriptase
R7824 T12350 T12351 punct (,Invitrogen
R7825 T12351 T12345 parataxis Invitrogen,transcriptase
R7826 T12352 T12351 punct ", ",Invitrogen
R7827 T12353 T12351 npadvmod Carlsbad,Invitrogen
R7828 T12354 T12351 punct ", ",Invitrogen
R7829 T12355 T12351 npadvmod CA,Invitrogen
R7830 T12356 T12351 punct ", ",Invitrogen
R7831 T12357 T12351 npadvmod USA,Invitrogen
R7832 T12358 T12351 punct ),Invitrogen
R7833 T12359 T12327 punct ", ",produced
R7834 T12360 T12327 prep according,produced
R7835 T12361 T12360 prep to,according
R7836 T12362 T12363 det the,manufacturer
R7837 T12363 T12364 poss manufacturer,recommendations
R7838 T12364 T12361 pobj recommendations,to
R7839 T12365 T12363 case 's,manufacturer
R7840 T12366 T12327 punct .,produced
R7841 T12368 T12369 nummod One,fifth
R7842 T12369 T12373 nsubjpass fifth,used
R7843 T12370 T12369 punct -,fifth
R7844 T12371 T12369 nummod twenty,fifth
R7845 T12372 T12369 punct -,fifth
R7846 T12374 T12369 prep of,fifth
R7847 T12375 T12376 det the,cDNA
R7848 T12376 T12374 pobj cDNA,of
R7849 T12377 T12376 amod resulting,cDNA
R7850 T12378 T12373 auxpass was,used
R7851 T12379 T12373 prep as,used
R7852 T12380 T12379 pobj template,as
R7853 T12381 T12373 prep in,used
R7854 T12382 T12383 amod subsequent,reactions
R7855 T12383 T12381 pobj reactions,in
R7856 T12384 T12383 compound PCR,reactions
R7857 T12385 T12373 punct .,used
R7858 T12387 T12388 compound PCR,amplification
R7859 T12388 T12389 nsubjpass amplification,performed
R7860 T12390 T12388 amod biased,amplification
R7861 T12391 T12390 prep towards,biased
R7862 T12392 T12393 nmod class,receptors
R7863 T12393 T12391 pobj receptors,towards
R7864 T12394 T12392 nummod I,class
R7865 T12395 T12393 amod olfactory,receptors
R7866 T12396 T12389 auxpass was,performed
R7867 T12397 T12389 advcl using,performed
R7868 T12398 T12399 amod degenerate,P26
R7869 T12399 T12397 dobj P26,using
R7870 T12400 T12399 compound primers,P26
R7871 T12401 T12402 punct [,17
R7872 T12402 T12399 parataxis 17,P26
R7873 T12403 T12402 punct ],17
R7874 T12404 T12399 cc and,P26
R7875 T12405 T12399 conj classI_R1,P26
R7876 T12406 T12407 punct (,GGRTTIADIRYIGGNGG
R7877 T12407 T12405 parataxis GGRTTIADIRYIGGNGG,classI_R1
R7878 T12408 T12407 nummod 5,GGRTTIADIRYIGGNGG
R7879 T12409 T12408 punct ',5
R7880 T12410 T12407 punct -,GGRTTIADIRYIGGNGG
R7881 T12411 T12407 punct -,GGRTTIADIRYIGGNGG
R7882 T12412 T12407 nummod 3,GGRTTIADIRYIGGNGG
R7883 T12413 T12407 punct ',GGRTTIADIRYIGGNGG
R7884 T12414 T12407 punct ),GGRTTIADIRYIGGNGG
R7885 T12415 T12399 prep with,P26
R7886 T12416 T12417 det an,temperature
R7887 T12417 T12415 pobj temperature,with
R7888 T12418 T12417 amod annealing,temperature
R7889 T12419 T12417 prep of,temperature
R7890 T12420 T12421 nummod 44,°C
R7891 T12421 T12419 pobj °C,of
R7892 T12422 T12389 punct .,performed
R7893 T12424 T12425 det The,product
R7894 T12425 T12426 nsubjpass product,cloned
R7895 T12427 T12426 auxpass was,cloned
R7896 T12428 T12429 punct (,Invitrogen
R7897 T12429 T12426 parataxis Invitrogen,cloned
R7898 T12430 T12431 compound TA,kit
R7899 T12431 T12429 dep kit,Invitrogen
R7900 T12432 T12431 compound cloning,kit
R7901 T12433 T12429 punct ", ",Invitrogen
R7902 T12434 T12429 punct ),Invitrogen
R7903 T12435 T12426 punct ", ",cloned
R7904 T12436 T12426 cc and,cloned
R7905 T12437 T12438 amod individual,clones
R7906 T12438 T12439 nsubjpass clones,sequenced
R7907 T12439 T12426 conj sequenced,cloned
R7908 T12440 T12439 auxpass were,sequenced
R7909 T12441 T12439 punct .,sequenced
R7910 T12443 T12444 amod Specific,primers
R7911 T12444 T12446 nsubjpass primers,given
R7912 T12445 T12444 compound PCR,primers
R7913 T12447 T12444 acl used,primers
R7914 T12448 T12449 aux to,confirm
R7915 T12449 T12447 advcl confirm,used
R7916 T12450 T12449 dobj expression,confirm
R7917 T12451 T12450 prep of,expression
R7918 T12452 T12453 amod individual,genes
R7919 T12453 T12451 pobj genes,of
R7920 T12454 T12455 amod olfactory,receptor
R7921 T12455 T12453 compound receptor,genes
R7922 T12456 T12446 auxpass are,given
R7923 T12457 T12446 prep in,given
R7924 T12458 T12459 amod Additional,file
R7925 T12459 T12457 pobj file,in
R7926 T12460 T12459 compound data,file
R7927 T12461 T12459 nummod 1,file
R7928 T12462 T12446 punct .,given
R7929 T12464 T12465 det Each,product
R7930 T12465 T12467 nsubjpass product,sequenced
R7931 T12466 T12465 compound PCR,product
R7932 T12468 T12467 auxpass was,sequenced
R7933 T12469 T12470 aux to,confirm
R7934 T12470 T12467 advcl confirm,sequenced
R7935 T12471 T12472 mark that,amplified
R7936 T12472 T12470 ccomp amplified,confirm
R7937 T12473 T12474 det the,gene
R7938 T12474 T12472 nsubjpass gene,amplified
R7939 T12475 T12474 amod expected,gene
R7940 T12476 T12474 cc and,gene
R7941 T12477 T12478 det no,others
R7942 T12478 T12474 conj others,gene
R7943 T12479 T12472 aux had,amplified
R7944 T12480 T12472 auxpass been,amplified
R7945 T12481 T12467 punct .,sequenced
R7946 T12483 T12484 compound Control,reactions
R7947 T12484 T12485 nsubj reactions,gave
R7948 T12486 T12484 prep on,reactions
R7949 T12487 T12488 det a,template
R7950 T12488 T12486 pobj template,on
R7951 T12489 T12488 acl made,template
R7952 T12490 T12489 prep by,made
R7953 T12491 T12490 pcomp omitting,by
R7954 T12492 T12493 amod reverse,transcriptase
R7955 T12493 T12491 dobj transcriptase,omitting
R7956 T12494 T12495 det no,product
R7957 T12495 T12485 dobj product,gave
R7958 T12496 T12485 punct ", ",gave
R7959 T12497 T12485 advcl indicating,gave
R7960 T12498 T12499 mark that,was
R7961 T12499 T12497 ccomp was,indicating
R7962 T12500 T12501 det the,preparation
R7963 T12501 T12499 nsubj preparation,was
R7964 T12502 T12501 compound RNA,preparation
R7965 T12503 T12499 acomp uncontaminated,was
R7966 T12504 T12503 prep by,uncontaminated
R7967 T12505 T12506 amod genomic,DNA
R7968 T12506 T12504 pobj DNA,by
R7969 T12507 T12485 punct .,gave
R7970 T12509 T12510 amod Relative,levels
R7971 T12510 T12512 nsubjpass levels,estimated
R7972 T12511 T12510 compound transcript,levels
R7973 T12513 T12512 auxpass were,estimated
R7974 T12514 T12512 advcl using,estimated
R7975 T12515 T12516 amod real,time
R7976 T12516 T12518 compound time,PCR
R7977 T12517 T12516 punct -,time
R7978 T12518 T12514 dobj PCR,using
R7979 T12519 T12514 prep according,using
R7980 T12520 T12519 prep to,according
R7981 T12521 T12522 compound Applied,Biosystems
R7982 T12522 T12523 poss Biosystems,protocols
R7983 T12523 T12520 pobj protocols,to
R7984 T12524 T12522 case ',Biosystems
R7985 T12525 T12514 punct ", ",using
R7986 T12526 T12527 mark with,given
R7987 T12527 T12514 advcl given,using
R7988 T12528 T12529 compound magnesium,concentration
R7989 T12529 T12527 nsubj concentration,given
R7990 T12530 T12529 punct ", ",concentration
R7991 T12531 T12532 compound primer,pair
R7992 T12532 T12529 conj pair,concentration
R7993 T12533 T12532 cc and,pair
R7994 T12534 T12535 amod fluorescent,probe
R7995 T12535 T12532 conj probe,pair
R7996 T12536 T12527 prep in,given
R7997 T12537 T12538 amod Additional,file
R7998 T12538 T12536 pobj file,in
R7999 T12539 T12538 compound data,file
R8000 T12540 T12538 nummod 2,file
R8001 T12541 T12512 punct .,estimated
R8002 T12543 T12544 det The,increase
R8003 T12544 T12545 nsubjpass increase,measured
R8004 T12546 T12544 prep in,increase
R8005 T12547 T12546 pobj fluorescence,in
R8006 T12548 T12544 prep during,increase
R8007 T12549 T12548 pobj thermocycling,during
R8008 T12550 T12545 auxpass was,measured
R8009 T12551 T12545 prep on,measured
R8010 T12552 T12553 det an,7900HT
R8011 T12553 T12551 pobj 7900HT,on
R8012 T12554 T12553 compound ABI,7900HT
R8013 T12555 T12553 compound PRISM,7900HT
R8014 T12556 T12545 punct .,measured
R8015 T12558 T12559 amod Standard,curves
R8016 T12559 T12560 nsubjpass curves,constructed
R8017 T12561 T12560 auxpass were,constructed
R8018 T12562 T12560 prep for,constructed
R8019 T12563 T12564 det each,pair
R8020 T12564 T12562 pobj pair,for
R8021 T12565 T12564 compound primer,pair
R8022 T12566 T12560 advcl using,constructed
R8023 T12567 T12568 compound triplicate,samples
R8024 T12568 T12566 dobj samples,using
R8025 T12569 T12568 prep of,samples
R8026 T12570 T12571 nmod mouse,DNA
R8027 T12571 T12569 pobj DNA,of
R8028 T12572 T12571 amod genomic,DNA
R8029 T12573 T12571 prep of,DNA
R8030 T12574 T12575 nummod nine,concentrations
R8031 T12575 T12573 pobj concentrations,of
R8032 T12576 T12575 amod known,concentrations
R8033 T12577 T12578 punct (,ng
R8034 T12578 T12575 parataxis ng,concentrations
R8035 T12579 T12578 dep range,ng
R8036 T12580 T12581 quantmod 0.01,100
R8037 T12581 T12578 nummod 100,ng
R8038 T12582 T12581 punct -,100
R8039 T12583 T12578 punct ", ",ng
R8040 T12584 T12578 cc or,ng
R8041 T12585 T12586 advmod about,"30,000"
R8042 T12586 T12589 nummod "30,000",copies
R8043 T12587 T12586 quantmod 3,"30,000"
R8044 T12588 T12586 punct -,"30,000"
R8045 T12589 T12578 conj copies,ng
R8046 T12590 T12589 prep of,copies
R8047 T12591 T12592 det the,genome
R8048 T12592 T12590 pobj genome,of
R8049 T12593 T12592 compound haploid,genome
R8050 T12594 T12578 punct ),ng
R8051 T12595 T12560 punct .,constructed
R8052 T12597 T12598 amod Relative,level
R8053 T12598 T12600 nsubjpass level,determined
R8054 T12599 T12598 compound expression,level
R8055 T12601 T12598 prep of,level
R8056 T12602 T12603 det each,gene
R8057 T12603 T12601 pobj gene,of
R8058 T12604 T12600 auxpass was,determined
R8059 T12605 T12600 prep by,determined
R8060 T12606 T12605 pcomp comparing,by
R8061 T12607 T12608 det the,Ct
R8062 T12608 T12606 dobj Ct,comparing
R8063 T12609 T12608 amod mean,Ct
R8064 T12610 T12608 punct (,Ct
R8065 T12611 T12608 appos cycle,Ct
R8066 T12612 T12613 advmod where,reaches
R8067 T12613 T12611 relcl reaches,cycle
R8068 T12614 T12613 nsubj fluorescence,reaches
R8069 T12615 T12616 det an,value
R8070 T12616 T12613 dobj value,reaches
R8071 T12617 T12616 amod arbitrary,value
R8072 T12618 T12616 compound threshold,value
R8073 T12619 T12608 punct ),Ct
R8074 T12620 T12608 acl obtained,Ct
R8075 T12621 T12620 prep with,obtained
R8076 T12622 T12623 nummod six,samples
R8077 T12623 T12621 pobj samples,with
R8078 T12624 T12623 compound replicate,samples
R8079 T12625 T12623 prep of,samples
R8080 T12626 T12627 amod reverse,transcribed
R8081 T12627 T12629 amod transcribed,RNA
R8082 T12628 T12627 punct -,transcribed
R8083 T12629 T12625 pobj RNA,of
R8084 T12630 T12606 prep to,comparing
R8085 T12631 T12632 det the,curve
R8086 T12632 T12630 pobj curve,to
R8087 T12633 T12632 amod standard,curve
R8088 T12634 T12632 prep for,curve
R8089 T12635 T12636 det the,primers
R8090 T12636 T12634 pobj primers,for
R8091 T12637 T12636 amod corresponding,primers
R8092 T12638 T12600 punct .,determined
R8093 T12640 T12641 amod Relative,levels
R8094 T12641 T12643 nsubjpass levels,measured
R8095 T12642 T12641 compound RNA,levels
R8096 T12644 T12641 prep of,levels
R8097 T12645 T12646 det a,gene
R8098 T12646 T12644 pobj gene,of
R8099 T12647 T12646 compound housekeeping,gene
R8100 T12648 T12646 punct ", ",gene
R8101 T12649 T12650 amod ribosomal,S16
R8102 T12650 T12646 appos S16,gene
R8103 T12651 T12643 punct ", ",measured
R8104 T12652 T12643 auxpass were,measured
R8105 T12653 T12654 mark as,described
R8106 T12654 T12643 advcl described,measured
R8107 T12655 T12654 advmod previously,described
R8108 T12656 T12657 punct [,52
R8109 T12657 T12654 parataxis 52,described
R8110 T12658 T12657 punct ],52
R8111 T12659 T12643 punct .,measured
R8112 T12661 T12662 compound Control,reactions
R8113 T12662 T12663 nsubj reactions,amplified
R8114 T12664 T12662 prep on,reactions
R8115 T12665 T12664 pobj template,on
R8116 T12666 T12665 acl prepared,template
R8117 T12667 T12666 prep by,prepared
R8118 T12668 T12667 pcomp omitting,by
R8119 T12669 T12670 amod reverse,transcriptase
R8120 T12670 T12668 dobj transcriptase,omitting
R8121 T12671 T12663 prep at,amplified
R8122 T12672 T12673 det a,level
R8123 T12673 T12671 pobj level,at
R8124 T12674 T12673 amod relative,level
R8125 T12675 T12673 prep of,level
R8126 T12676 T12677 quantmod 0.03,0.01
R8127 T12677 T12679 nummod 0.01,ng
R8128 T12678 T12677 punct ±,0.01
R8129 T12679 T12675 pobj ng,of
R8130 T12680 T12679 cc or,ng
R8131 T12681 T12679 conj less,ng
R8132 T12682 T12663 prep in,amplified
R8133 T12683 T12684 det each,case
R8134 T12684 T12682 pobj case,in
R8135 T12685 T12663 punct .,amplified
R8136 T12687 T12688 compound Expression,measurements
R8137 T12688 T12689 nsubjpass measurements,normalized
R8138 T12690 T12688 prep of,measurements
R8139 T12691 T12692 det the,genes
R8140 T12692 T12690 pobj genes,of
R8141 T12693 T12692 nummod seven,genes
R8142 T12694 T12689 auxpass were,normalized
R8143 T12695 T12689 prep for,normalized
R8144 T12696 T12697 det each,mouse
R8145 T12697 T12695 pobj mouse,for
R8146 T12698 T12699 mark so,were
R8147 T12699 T12689 advcl were,normalized
R8148 T12700 T12699 mark that,were
R8149 T12701 T12702 compound S16,levels
R8150 T12702 T12699 nsubj levels,were
R8151 T12703 T12699 acomp equal,were
R8152 T12704 T12703 prep to,equal
R8153 T12705 T12704 pobj 1,to
R8154 T12706 T12707 punct (,units
R8155 T12707 T12705 parataxis units,1
R8156 T12708 T12707 amod arbitrary,units
R8157 T12709 T12707 punct ),units
R8158 T12710 T12689 punct .,normalized