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PMC:329117 / 12107-15667 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T3520 0-1 DT denotes A
T3522 2-7 NN denotes class
T3523 8-9 CD denotes I
T3524 10-19 JJ denotes olfactory
T3525 20-28 NN denotes receptor
T3526 29-39 JJ denotes degenerate
T3521 40-46 NN denotes primer
T3527 47-55 VBZ denotes broadens
T3528 56-68 JJ denotes phylogenetic
T3529 69-81 NN denotes distribution
T3530 82-84 IN denotes of
T3531 85-94 VBN denotes confirmed
T3533 95-104 JJ denotes olfactory
T3534 105-113 NN denotes receptor
T3532 114-119 NNS denotes genes
T3535 119-383 sentence denotes Previous analyses of the mammalian olfactory receptor family define two major phylogenetic clades, referred to as class I and II olfactory receptors, and suggest that class I olfactory receptors are more similar to fish olfactory receptors than are class IIs [5].
T3536 120-128 JJ denotes Previous
T3537 129-137 NNS denotes analyses
T3539 138-140 IN denotes of
T3540 141-144 DT denotes the
T3542 145-154 JJ denotes mammalian
T3543 155-164 JJ denotes olfactory
T3544 165-173 NN denotes receptor
T3541 174-180 NN denotes family
T3538 181-187 VBZ denotes define
T3545 188-191 CD denotes two
T3547 192-197 JJ denotes major
T3548 198-210 JJ denotes phylogenetic
T3546 211-217 NNS denotes clades
T3549 217-219 , denotes ,
T3550 219-227 VBN denotes referred
T3551 228-230 IN denotes to
T3552 231-233 IN denotes as
T3553 234-239 NN denotes class
T3554 240-241 CD denotes I
T3556 242-245 CC denotes and
T3557 246-248 CD denotes II
T3558 249-258 JJ denotes olfactory
T3555 259-268 NNS denotes receptors
T3559 268-270 , denotes ,
T3560 270-273 CC denotes and
T3561 274-281 VBP denotes suggest
T3562 282-286 IN denotes that
T3564 287-292 NN denotes class
T3566 293-294 CD denotes I
T3567 295-304 JJ denotes olfactory
T3565 305-314 NNS denotes receptors
T3563 315-318 VBP denotes are
T3568 319-323 RBR denotes more
T3569 324-331 JJ denotes similar
T3570 332-334 IN denotes to
T3571 335-339 NN denotes fish
T3573 340-349 JJ denotes olfactory
T3572 350-359 NNS denotes receptors
T3574 360-364 IN denotes than
T3575 365-368 VBP denotes are
T3576 369-374 NN denotes class
T3577 375-378 NNS denotes IIs
T3578 379-380 -LRB- denotes [
T3579 380-381 CD denotes 5
T3580 381-382 -RRB- denotes ]
T3581 382-383 . denotes .
T3582 383-742 sentence denotes Figure 1 illustrates the phylogenetic diversity of our cDNA collection, showing that we have confirmed expression of at least one olfactory receptor gene in each major clade of the class II olfactory receptor genes, or 391 out of 983 (40%) of all intact class II olfactory receptor genes where full-length genomic sequence data are available (blue branches).
T3583 384-390 NN denotes Figure
T3585 391-392 CD denotes 1
T3584 393-404 VBZ denotes illustrates
T3586 405-408 DT denotes the
T3588 409-421 JJ denotes phylogenetic
T3587 422-431 NN denotes diversity
T3589 432-434 IN denotes of
T3590 435-438 PRP$ denotes our
T3592 439-443 NN denotes cDNA
T3591 444-454 NN denotes collection
T3593 454-456 , denotes ,
T3594 456-463 VBG denotes showing
T3595 464-468 IN denotes that
T3597 469-471 PRP denotes we
T3598 472-476 VBP denotes have
T3596 477-486 VBN denotes confirmed
T3599 487-497 NN denotes expression
T3600 498-500 IN denotes of
T3601 501-503 RB denotes at
T3603 504-509 RBS denotes least
T3602 510-513 CD denotes one
T3605 514-523 JJ denotes olfactory
T3606 524-532 NN denotes receptor
T3604 533-537 NN denotes gene
T3607 538-540 IN denotes in
T3608 541-545 DT denotes each
T3610 546-551 JJ denotes major
T3609 552-557 NN denotes clade
T3611 558-560 IN denotes of
T3612 561-564 DT denotes the
T3614 565-570 NN denotes class
T3615 571-573 CD denotes II
T3616 574-583 JJ denotes olfactory
T3617 584-592 NN denotes receptor
T3613 593-598 NNS denotes genes
T3618 598-600 , denotes ,
T3619 600-602 CC denotes or
T3620 603-606 CD denotes 391
T3622 607-610 IN denotes out
T3623 611-613 IN denotes of
T3621 614-617 CD denotes 983
T3624 618-619 -LRB- denotes (
T3626 619-621 CD denotes 40
T3625 621-622 NN denotes %
T3627 622-623 -RRB- denotes )
T3628 624-626 IN denotes of
T3629 627-630 DT denotes all
T3631 631-637 JJ denotes intact
T3632 638-643 NN denotes class
T3633 644-646 CD denotes II
T3634 647-656 JJ denotes olfactory
T3635 657-665 NN denotes receptor
T3630 666-671 NNS denotes genes
T3636 672-677 WRB denotes where
T3638 678-682 JJ denotes full
T3640 682-683 HYPH denotes -
T3639 683-689 NN denotes length
T3642 690-697 JJ denotes genomic
T3643 698-706 NN denotes sequence
T3641 707-711 NNS denotes data
T3637 712-715 VBP denotes are
T3644 716-725 JJ denotes available
T3645 726-727 -LRB- denotes (
T3647 727-731 JJ denotes blue
T3646 732-740 NNS denotes branches
T3648 740-741 -RRB- denotes )
T3649 741-742 . denotes .
T3650 742-835 sentence denotes The screen thus appears relatively unbiased in its coverage of class II olfactory receptors.
T3651 743-746 DT denotes The
T3652 747-753 NN denotes screen
T3654 754-758 RB denotes thus
T3653 759-766 VBZ denotes appears
T3655 767-777 RB denotes relatively
T3656 778-786 JJ denotes unbiased
T3657 787-789 IN denotes in
T3658 790-793 PRP$ denotes its
T3659 794-802 NN denotes coverage
T3660 803-805 IN denotes of
T3661 806-811 NN denotes class
T3663 812-814 CD denotes II
T3664 815-824 JJ denotes olfactory
T3662 825-834 NNS denotes receptors
T3665 834-835 . denotes .
T3666 835-950 sentence denotes However, our random screen provided cDNAs for only two out of 124 intact, full-length class I olfactory receptors.
T3667 836-843 RB denotes However
T3669 843-845 , denotes ,
T3670 845-848 PRP$ denotes our
T3672 849-855 JJ denotes random
T3671 856-862 NN denotes screen
T3668 863-871 VBD denotes provided
T3673 872-877 NNS denotes cDNAs
T3674 878-881 IN denotes for
T3675 882-886 RB denotes only
T3677 887-890 CD denotes two
T3678 891-894 IN denotes out
T3679 895-897 IN denotes of
T3676 898-901 CD denotes 124
T3681 902-908 JJ denotes intact
T3682 908-910 , denotes ,
T3683 910-914 JJ denotes full
T3685 914-915 HYPH denotes -
T3684 915-921 NN denotes length
T3686 922-927 NN denotes class
T3687 928-929 CD denotes I
T3688 930-939 JJ denotes olfactory
T3680 940-949 NNS denotes receptors
T3689 949-950 . denotes .
T3690 950-1133 sentence denotes In an attempt to broaden the phylogenetic coverage of our hybridization screen, we used additional degenerate probes on the adult library and screened an embryonic library (Table 2).
T3691 951-953 IN denotes In
T3693 954-956 DT denotes an
T3694 957-964 NN denotes attempt
T3695 965-967 TO denotes to
T3696 968-975 VB denotes broaden
T3697 976-979 DT denotes the
T3699 980-992 JJ denotes phylogenetic
T3698 993-1001 NN denotes coverage
T3700 1002-1004 IN denotes of
T3701 1005-1008 PRP$ denotes our
T3703 1009-1022 NN denotes hybridization
T3702 1023-1029 NN denotes screen
T3704 1029-1031 , denotes ,
T3705 1031-1033 PRP denotes we
T3692 1034-1038 VBD denotes used
T3706 1039-1049 JJ denotes additional
T3708 1050-1060 JJ denotes degenerate
T3707 1061-1067 NNS denotes probes
T3709 1068-1070 IN denotes on
T3710 1071-1074 DT denotes the
T3712 1075-1080 JJ denotes adult
T3711 1081-1088 NN denotes library
T3713 1089-1092 CC denotes and
T3714 1093-1101 VBD denotes screened
T3715 1102-1104 DT denotes an
T3717 1105-1114 JJ denotes embryonic
T3716 1115-1122 NN denotes library
T3718 1123-1124 -LRB- denotes (
T3719 1124-1129 NN denotes Table
T3720 1130-1131 CD denotes 2
T3721 1131-1132 -RRB- denotes )
T3722 1132-1133 . denotes .
T3723 1133-1216 sentence denotes These experiments did not increase the diversity of clones identified (not shown).
T3724 1134-1139 DT denotes These
T3725 1140-1151 NNS denotes experiments
T3727 1152-1155 VBD denotes did
T3728 1156-1159 RB denotes not
T3726 1160-1168 VB denotes increase
T3729 1169-1172 DT denotes the
T3730 1173-1182 NN denotes diversity
T3731 1183-1185 IN denotes of
T3732 1186-1192 NNS denotes clones
T3733 1193-1203 VBN denotes identified
T3734 1204-1205 -LRB- denotes (
T3736 1205-1208 RB denotes not
T3735 1209-1214 VBN denotes shown
T3737 1214-1215 -RRB- denotes )
T3738 1215-1216 . denotes .
T3739 1216-1369 sentence denotes This severe class I underrepresentation could be due to experimental bias - a consequence of using degenerate primers to create our hybridization probe.
T3740 1217-1221 DT denotes This
T3742 1222-1228 JJ denotes severe
T3743 1229-1234 NN denotes class
T3744 1235-1236 CD denotes I
T3741 1237-1256 NN denotes underrepresentation
T3746 1257-1262 MD denotes could
T3745 1263-1265 VB denotes be
T3747 1266-1269 IN denotes due
T3748 1270-1272 IN denotes to
T3749 1273-1285 JJ denotes experimental
T3750 1286-1290 NN denotes bias
T3751 1291-1292 : denotes -
T3752 1293-1294 DT denotes a
T3753 1295-1306 NN denotes consequence
T3754 1307-1309 IN denotes of
T3755 1310-1315 VBG denotes using
T3756 1316-1326 JJ denotes degenerate
T3757 1327-1334 NNS denotes primers
T3758 1335-1337 TO denotes to
T3759 1338-1344 VB denotes create
T3760 1345-1348 PRP$ denotes our
T3762 1349-1362 NN denotes hybridization
T3761 1363-1368 NN denotes probe
T3763 1368-1369 . denotes .
T3764 1369-1470 sentence denotes Alternatively, class I genes might be expressed at extremely low levels in the olfactory epithelium.
T3765 1370-1383 RB denotes Alternatively
T3767 1383-1385 , denotes ,
T3768 1385-1390 NN denotes class
T3770 1391-1392 CD denotes I
T3769 1393-1398 NNS denotes genes
T3771 1399-1404 MD denotes might
T3772 1405-1407 VB denotes be
T3766 1408-1417 VBN denotes expressed
T3773 1418-1420 IN denotes at
T3774 1421-1430 RB denotes extremely
T3775 1431-1434 JJ denotes low
T3776 1435-1441 NNS denotes levels
T3777 1442-1444 IN denotes in
T3778 1445-1448 DT denotes the
T3780 1449-1458 JJ denotes olfactory
T3779 1459-1469 NN denotes epithelium
T3781 1469-1470 . denotes .
T3782 1470-1743 sentence denotes In order to determine whether class I olfactory receptors are expressed in the olfactory epithelium, we designed a reverse-strand degenerate primer to recognize a motif in transmembrane domain 7 (PP{V/M/A/T}{F/L/I/M}NP) enriched among class I olfactory receptor sequences.
T3783 1471-1473 IN denotes In
T3785 1474-1479 NN denotes order
T3786 1480-1482 TO denotes to
T3787 1483-1492 VB denotes determine
T3788 1493-1500 IN denotes whether
T3790 1501-1506 NN denotes class
T3792 1507-1508 CD denotes I
T3793 1509-1518 JJ denotes olfactory
T3791 1519-1528 NNS denotes receptors
T3794 1529-1532 VBP denotes are
T3789 1533-1542 VBN denotes expressed
T3795 1543-1545 IN denotes in
T3796 1546-1549 DT denotes the
T3798 1550-1559 JJ denotes olfactory
T3797 1560-1570 NN denotes epithelium
T3799 1570-1572 , denotes ,
T3800 1572-1574 PRP denotes we
T3784 1575-1583 VBD denotes designed
T3801 1584-1585 DT denotes a
T3803 1586-1593 JJ denotes reverse
T3805 1593-1594 HYPH denotes -
T3804 1594-1600 NN denotes strand
T3806 1601-1611 JJ denotes degenerate
T3802 1612-1618 NN denotes primer
T3807 1619-1621 TO denotes to
T3808 1622-1631 VB denotes recognize
T3809 1632-1633 DT denotes a
T3810 1634-1639 NN denotes motif
T3811 1640-1642 IN denotes in
T3812 1643-1656 NN denotes transmembrane
T3813 1657-1663 NN denotes domain
T3814 1664-1665 CD denotes 7
T3815 1666-1667 -LRB- denotes (
T3817 1667-1669 NN denotes PP
T3818 1669-1670 -LRB- denotes {
T3819 1670-1671 NN denotes V
T3821 1671-1672 HYPH denotes /
T3822 1672-1673 NN denotes M
T3823 1673-1674 HYPH denotes /
T3824 1674-1675 NN denotes A
T3825 1675-1676 HYPH denotes /
T3820 1676-1677 NN denotes T
T3826 1677-1678 RBR denotes }
T3827 1678-1679 -LRB- denotes {
T3828 1679-1680 NN denotes F
T3830 1680-1681 HYPH denotes /
T3831 1681-1682 NN denotes L
T3832 1682-1683 HYPH denotes /
T3833 1683-1684 NN denotes I
T3834 1684-1685 HYPH denotes /
T3829 1685-1686 NN denotes M
T3835 1686-1687 RBR denotes }
T3816 1687-1689 NN denotes NP
T3836 1689-1690 -RRB- denotes )
T3837 1691-1699 VBN denotes enriched
T3838 1700-1705 IN denotes among
T3839 1706-1711 NN denotes class
T3841 1712-1713 CD denotes I
T3842 1714-1723 JJ denotes olfactory
T3843 1724-1732 NN denotes receptor
T3840 1733-1742 NNS denotes sequences
T3844 1742-1743 . denotes .
T3845 1743-1970 sentence denotes Most of the motif is shared among all olfactory receptors, but the first proline residue (at the primer's 3' end) is found in 121 out of 124 (98%) intact class I genes compared to only 37 out of 983 (4%) intact class II genes.
T3846 1744-1748 JJS denotes Most
T3848 1749-1751 IN denotes of
T3849 1752-1755 DT denotes the
T3850 1756-1761 NN denotes motif
T3851 1762-1764 VBZ denotes is
T3847 1765-1771 VBN denotes shared
T3852 1772-1777 IN denotes among
T3853 1778-1781 DT denotes all
T3855 1782-1791 JJ denotes olfactory
T3854 1792-1801 NNS denotes receptors
T3856 1801-1803 , denotes ,
T3857 1803-1806 CC denotes but
T3858 1807-1810 DT denotes the
T3860 1811-1816 JJ denotes first
T3861 1817-1824 NN denotes proline
T3859 1825-1832 NN denotes residue
T3863 1833-1834 -LRB- denotes (
T3864 1834-1836 IN denotes at
T3865 1837-1840 DT denotes the
T3866 1841-1847 NN denotes primer
T3868 1847-1849 POS denotes 's
T3869 1850-1851 CD denotes 3
T3870 1851-1852 SYM denotes '
T3867 1853-1856 NN denotes end
T3871 1856-1857 -RRB- denotes )
T3872 1858-1860 VBZ denotes is
T3862 1861-1866 VBN denotes found
T3873 1867-1869 IN denotes in
T3874 1870-1873 CD denotes 121
T3876 1874-1877 IN denotes out
T3877 1878-1880 IN denotes of
T3875 1881-1884 CD denotes 124
T3879 1885-1886 -LRB- denotes (
T3881 1886-1888 CD denotes 98
T3880 1888-1889 NN denotes %
T3882 1889-1890 -RRB- denotes )
T3883 1891-1897 JJ denotes intact
T3884 1898-1903 NN denotes class
T3885 1904-1905 CD denotes I
T3878 1906-1911 NNS denotes genes
T3886 1912-1920 VBN denotes compared
T3887 1921-1923 IN denotes to
T3888 1924-1928 RB denotes only
T3890 1929-1931 CD denotes 37
T3891 1932-1935 IN denotes out
T3892 1936-1938 IN denotes of
T3889 1939-1942 CD denotes 983
T3894 1943-1944 -LRB- denotes (
T3896 1944-1945 CD denotes 4
T3895 1945-1946 NN denotes %
T3897 1946-1947 -RRB- denotes )
T3898 1948-1954 JJ denotes intact
T3899 1955-1960 NN denotes class
T3900 1961-1963 CD denotes II
T3893 1964-1969 NNS denotes genes
T3901 1969-1970 . denotes .
T3902 1970-2366 sentence denotes When combined with another olfactory receptor degenerate primer, P26 [17], this primer preferentially amplifies class I olfactory receptors from mouse genomic DNA: of 33 sequenced, cloned PCR products, 17 represented seven different class I olfactory receptors, six represented three different class II olfactory receptors, and ten represented five different non-olfactory receptor contaminants.
T3903 1971-1975 WRB denotes When
T3904 1976-1984 VBN denotes combined
T3906 1985-1989 IN denotes with
T3907 1990-1997 DT denotes another
T3909 1998-2007 JJ denotes olfactory
T3910 2008-2016 NN denotes receptor
T3911 2017-2027 JJ denotes degenerate
T3908 2028-2034 NN denotes primer
T3912 2034-2036 , denotes ,
T3913 2036-2039 NN denotes P26
T3914 2040-2041 -LRB- denotes [
T3915 2041-2043 CD denotes 17
T3916 2043-2044 -RRB- denotes ]
T3917 2044-2046 , denotes ,
T3918 2046-2050 DT denotes this
T3919 2051-2057 NN denotes primer
T3920 2058-2072 RB denotes preferentially
T3905 2073-2082 VBZ denotes amplifies
T3922 2083-2088 NN denotes class
T3924 2089-2090 CD denotes I
T3925 2091-2100 JJ denotes olfactory
T3923 2101-2110 NNS denotes receptors
T3926 2111-2115 IN denotes from
T3927 2116-2121 NN denotes mouse
T3929 2122-2129 JJ denotes genomic
T3928 2130-2133 NN denotes DNA
T3930 2133-2135 : denotes :
T3931 2135-2137 IN denotes of
T3932 2138-2140 CD denotes 33
T3934 2141-2150 JJ denotes sequenced
T3935 2150-2152 , denotes ,
T3936 2152-2158 JJ denotes cloned
T3937 2159-2162 NN denotes PCR
T3933 2163-2171 NNS denotes products
T3938 2171-2173 , denotes ,
T3939 2173-2175 CD denotes 17
T3921 2176-2187 VBD denotes represented
T3940 2188-2193 CD denotes seven
T3942 2194-2203 JJ denotes different
T3943 2204-2209 NN denotes class
T3944 2210-2211 CD denotes I
T3945 2212-2221 JJ denotes olfactory
T3941 2222-2231 NNS denotes receptors
T3946 2231-2233 , denotes ,
T3947 2233-2236 CD denotes six
T3948 2237-2248 VBD denotes represented
T3949 2249-2254 CD denotes three
T3951 2255-2264 JJ denotes different
T3952 2265-2270 NN denotes class
T3953 2271-2273 CD denotes II
T3954 2274-2283 JJ denotes olfactory
T3950 2284-2293 NNS denotes receptors
T3955 2293-2295 , denotes ,
T3956 2295-2298 CC denotes and
T3957 2299-2302 CD denotes ten
T3958 2303-2314 VBD denotes represented
T3959 2315-2319 CD denotes five
T3961 2320-2329 JJ denotes different
T3962 2330-2343 JJ denotes non-olfactory
T3963 2344-2352 NN denotes receptor
T3960 2353-2365 NNS denotes contaminants
T3964 2365-2366 . denotes .
T3965 2366-2593 sentence denotes Degenerate PCR, cloning, and sequencing from reverse-transcribed olfactory epithelium RNA showed that at least seven class I olfactory receptors are expressed, as well as one additional class II gene (colored red in Figure 1).
T3966 2367-2377 JJ denotes Degenerate
T3967 2378-2381 NN denotes PCR
T3969 2381-2383 , denotes ,
T3970 2383-2390 NN denotes cloning
T3971 2390-2392 , denotes ,
T3972 2392-2395 CC denotes and
T3973 2396-2406 NN denotes sequencing
T3974 2407-2411 IN denotes from
T3975 2412-2419 JJ denotes reverse
T3977 2419-2420 HYPH denotes -
T3976 2420-2431 VBN denotes transcribed
T3979 2432-2441 JJ denotes olfactory
T3980 2442-2452 NN denotes epithelium
T3978 2453-2456 NN denotes RNA
T3968 2457-2463 VBD denotes showed
T3981 2464-2468 IN denotes that
T3983 2469-2471 RB denotes at
T3985 2472-2477 RBS denotes least
T3984 2478-2483 CD denotes seven
T3987 2484-2489 NN denotes class
T3988 2490-2491 CD denotes I
T3989 2492-2501 JJ denotes olfactory
T3986 2502-2511 NNS denotes receptors
T3990 2512-2515 VBP denotes are
T3982 2516-2525 VBN denotes expressed
T3991 2525-2527 , denotes ,
T3992 2527-2529 RB denotes as
T3994 2530-2534 RB denotes well
T3993 2535-2537 IN denotes as
T3996 2538-2541 CD denotes one
T3997 2542-2552 JJ denotes additional
T3998 2553-2558 NN denotes class
T3999 2559-2561 CD denotes II
T3995 2562-2566 NN denotes gene
T4000 2567-2568 -LRB- denotes (
T4001 2568-2575 VBN denotes colored
T4002 2576-2579 JJ denotes red
T4003 2580-2582 IN denotes in
T4004 2583-2589 NN denotes Figure
T4005 2590-2591 CD denotes 1
T4006 2591-2592 -RRB- denotes )
T4007 2592-2593 . denotes .
T4008 2593-2808 sentence denotes However, no products could be obtained from the adult or the fetal olfactory epithelium cDNA libraries using the class I primer, suggesting that the libraries contain very low levels of class I olfactory receptors.
T4009 2594-2601 RB denotes However
T4011 2601-2603 , denotes ,
T4012 2603-2605 DT denotes no
T4013 2606-2614 NNS denotes products
T4014 2615-2620 MD denotes could
T4015 2621-2623 VB denotes be
T4010 2624-2632 VBN denotes obtained
T4016 2633-2637 IN denotes from
T4017 2638-2641 DT denotes the
T4018 2642-2647 JJ denotes adult
T4019 2648-2650 CC denotes or
T4020 2651-2654 DT denotes the
T4021 2655-2660 JJ denotes fetal
T4023 2661-2670 JJ denotes olfactory
T4024 2671-2681 NN denotes epithelium
T4025 2682-2686 NN denotes cDNA
T4022 2687-2696 NNS denotes libraries
T4026 2697-2702 VBG denotes using
T4027 2703-2706 DT denotes the
T4029 2707-2712 NN denotes class
T4030 2713-2714 CD denotes I
T4028 2715-2721 NN denotes primer
T4031 2721-2723 , denotes ,
T4032 2723-2733 VBG denotes suggesting
T4033 2734-2738 IN denotes that
T4035 2739-2742 DT denotes the
T4036 2743-2752 NNS denotes libraries
T4034 2753-2760 VBP denotes contain
T4037 2761-2765 RB denotes very
T4038 2766-2769 JJ denotes low
T4039 2770-2776 NNS denotes levels
T4040 2777-2779 IN denotes of
T4041 2780-2785 NN denotes class
T4043 2786-2787 CD denotes I
T4044 2788-2797 JJ denotes olfactory
T4042 2798-2807 NNS denotes receptors
T4045 2807-2808 . denotes .
T4046 2808-3092 sentence denotes We also confirmed expression of nine additional olfactory receptors (three class I and six class II, colored red in Figure 1) from subclades that were poorly represented in our cDNA screen using gene-specific primer pairs to amplify cDNA library or reverse-transcribed RNA templates.
T4047 2809-2811 PRP denotes We
T4049 2812-2816 RB denotes also
T4048 2817-2826 VBD denotes confirmed
T4050 2827-2837 NN denotes expression
T4051 2838-2840 IN denotes of
T4052 2841-2845 CD denotes nine
T4054 2846-2856 JJ denotes additional
T4055 2857-2866 JJ denotes olfactory
T4053 2867-2876 NNS denotes receptors
T4056 2877-2878 -LRB- denotes (
T4057 2878-2883 CD denotes three
T4058 2884-2889 NN denotes class
T4059 2890-2891 CD denotes I
T4060 2892-2895 CC denotes and
T4061 2896-2899 CD denotes six
T4062 2900-2905 NN denotes class
T4063 2906-2908 CD denotes II
T4064 2908-2910 , denotes ,
T4065 2910-2917 VBN denotes colored
T4066 2918-2921 JJ denotes red
T4067 2922-2924 IN denotes in
T4068 2925-2931 NN denotes Figure
T4069 2932-2933 CD denotes 1
T4070 2933-2934 -RRB- denotes )
T4071 2935-2939 IN denotes from
T4072 2940-2949 NNS denotes subclades
T4073 2950-2954 WDT denotes that
T4075 2955-2959 VBD denotes were
T4076 2960-2966 RB denotes poorly
T4074 2967-2978 VBN denotes represented
T4077 2979-2981 IN denotes in
T4078 2982-2985 PRP$ denotes our
T4080 2986-2990 NN denotes cDNA
T4079 2991-2997 NN denotes screen
T4081 2998-3003 VBG denotes using
T4082 3004-3008 NN denotes gene
T4084 3008-3009 HYPH denotes -
T4083 3009-3017 JJ denotes specific
T4086 3018-3024 NN denotes primer
T4085 3025-3030 NNS denotes pairs
T4087 3031-3033 TO denotes to
T4088 3034-3041 VB denotes amplify
T4089 3042-3046 NN denotes cDNA
T4090 3047-3054 NN denotes library
T4091 3055-3057 CC denotes or
T4092 3058-3065 JJ denotes reverse
T4094 3065-3066 HYPH denotes -
T4093 3066-3077 VBN denotes transcribed
T4096 3078-3081 NN denotes RNA
T4095 3082-3091 NNS denotes templates
T4097 3091-3092 . denotes .
T4098 3092-3231 sentence denotes For two of the class I genes we had shown to be expressed, we determined relative transcript levels using quantitative RT-PCR (see below).
T4099 3093-3096 IN denotes For
T4101 3097-3100 CD denotes two
T4102 3101-3103 IN denotes of
T4103 3104-3107 DT denotes the
T4105 3108-3113 NN denotes class
T4106 3114-3115 CD denotes I
T4104 3116-3121 NNS denotes genes
T4107 3122-3124 PRP denotes we
T4109 3125-3128 VBD denotes had
T4108 3129-3134 VBN denotes shown
T4110 3135-3137 TO denotes to
T4112 3138-3140 VB denotes be
T4111 3141-3150 VBN denotes expressed
T4113 3150-3152 , denotes ,
T4114 3152-3154 PRP denotes we
T4100 3155-3165 VBD denotes determined
T4115 3166-3174 JJ denotes relative
T4117 3175-3185 NN denotes transcript
T4116 3186-3192 NNS denotes levels
T4118 3193-3198 VBG denotes using
T4119 3199-3211 JJ denotes quantitative
T4121 3212-3214 NN denotes RT
T4122 3214-3215 HYPH denotes -
T4120 3215-3218 NN denotes PCR
T4123 3219-3220 -LRB- denotes (
T4124 3220-3223 VB denotes see
T4125 3224-3229 RB denotes below
T4126 3229-3230 -RRB- denotes )
T4127 3230-3231 . denotes .
T4128 3231-3560 sentence denotes Expression levels were similar to those observed for genes that were represented in our cDNA collection, suggesting that class I olfactory receptors are not under-represented in the olfactory epithelium, and that the dearth of class I cDNAs in our screen is likely to be due to bias in the libraries and/or hybridization probes.
T4129 3232-3242 NN denotes Expression
T4130 3243-3249 NNS denotes levels
T4131 3250-3254 VBD denotes were
T4132 3255-3262 JJ denotes similar
T4133 3263-3265 IN denotes to
T4134 3266-3271 DT denotes those
T4135 3272-3280 VBN denotes observed
T4136 3281-3284 IN denotes for
T4137 3285-3290 NNS denotes genes
T4138 3291-3295 WDT denotes that
T4140 3296-3300 VBD denotes were
T4139 3301-3312 VBN denotes represented
T4141 3313-3315 IN denotes in
T4142 3316-3319 PRP$ denotes our
T4144 3320-3324 NN denotes cDNA
T4143 3325-3335 NN denotes collection
T4145 3335-3337 , denotes ,
T4146 3337-3347 VBG denotes suggesting
T4147 3348-3352 IN denotes that
T4149 3353-3358 NN denotes class
T4151 3359-3360 CD denotes I
T4152 3361-3370 JJ denotes olfactory
T4150 3371-3380 NNS denotes receptors
T4153 3381-3384 VBP denotes are
T4154 3385-3388 RB denotes not
T4155 3389-3394 RB denotes under
T4156 3394-3395 HYPH denotes -
T4148 3395-3406 VBN denotes represented
T4157 3407-3409 IN denotes in
T4158 3410-3413 DT denotes the
T4160 3414-3423 JJ denotes olfactory
T4159 3424-3434 NN denotes epithelium
T4161 3434-3436 , denotes ,
T4162 3436-3439 CC denotes and
T4163 3440-3444 IN denotes that
T4165 3445-3448 DT denotes the
T4166 3449-3455 NN denotes dearth
T4167 3456-3458 IN denotes of
T4168 3459-3464 NN denotes class
T4170 3465-3466 CD denotes I
T4169 3467-3472 NNS denotes cDNAs
T4171 3473-3475 IN denotes in
T4172 3476-3479 PRP$ denotes our
T4173 3480-3486 NN denotes screen
T4164 3487-3489 VBZ denotes is
T4174 3490-3496 JJ denotes likely
T4175 3497-3499 TO denotes to
T4176 3500-3502 VB denotes be
T4177 3503-3506 IN denotes due
T4178 3507-3509 IN denotes to
T4179 3510-3514 NN denotes bias
T4180 3515-3517 IN denotes in
T4181 3518-3521 DT denotes the
T4182 3522-3531 NNS denotes libraries
T4183 3532-3535 CC denotes and
T4184 3535-3536 HYPH denotes /
T4185 3536-3538 CC denotes or
T4186 3539-3552 NN denotes hybridization
T4187 3553-3559 NNS denotes probes
T4188 3559-3560 . denotes .
R1950 T3520 T3521 det A,primer
R1951 T3521 T3527 nsubj primer,broadens
R1952 T3522 T3521 nmod class,primer
R1953 T3523 T3522 nummod I,class
R1954 T3524 T3525 amod olfactory,receptor
R1955 T3525 T3521 nmod receptor,primer
R1956 T3526 T3521 amod degenerate,primer
R1957 T3528 T3529 amod phylogenetic,distribution
R1958 T3529 T3527 dobj distribution,broadens
R1959 T3530 T3529 prep of,distribution
R1960 T3531 T3532 amod confirmed,genes
R1961 T3532 T3530 pobj genes,of
R1962 T3533 T3534 amod olfactory,receptor
R1963 T3534 T3532 compound receptor,genes
R1964 T3536 T3537 amod Previous,analyses
R1965 T3537 T3538 nsubj analyses,define
R1966 T3539 T3537 prep of,analyses
R1967 T3540 T3541 det the,family
R1968 T3541 T3539 pobj family,of
R1969 T3542 T3541 amod mammalian,family
R1970 T3543 T3544 amod olfactory,receptor
R1971 T3544 T3541 compound receptor,family
R1972 T3545 T3546 nummod two,clades
R1973 T3546 T3538 dobj clades,define
R1974 T3547 T3546 amod major,clades
R1975 T3548 T3546 amod phylogenetic,clades
R1976 T3549 T3546 punct ", ",clades
R1977 T3550 T3546 acl referred,clades
R1978 T3551 T3550 prep to,referred
R1979 T3552 T3550 prep as,referred
R1980 T3553 T3554 nmod class,I
R1981 T3554 T3555 nummod I,receptors
R1982 T3555 T3552 pobj receptors,as
R1983 T3556 T3554 cc and,I
R1984 T3557 T3554 conj II,I
R1985 T3558 T3555 amod olfactory,receptors
R1986 T3559 T3538 punct ", ",define
R1987 T3560 T3538 cc and,define
R1988 T3561 T3538 conj suggest,define
R1989 T3562 T3563 mark that,are
R1990 T3563 T3561 ccomp are,suggest
R1991 T3564 T3565 nmod class,receptors
R1992 T3565 T3563 nsubj receptors,are
R1993 T3566 T3564 nummod I,class
R1994 T3567 T3565 amod olfactory,receptors
R1995 T3568 T3569 advmod more,similar
R1996 T3569 T3563 acomp similar,are
R1997 T3570 T3569 prep to,similar
R1998 T3571 T3572 nmod fish,receptors
R1999 T3572 T3570 pobj receptors,to
R2000 T3573 T3572 amod olfactory,receptors
R2001 T3574 T3575 mark than,are
R2002 T3575 T3569 advcl are,similar
R2003 T3576 T3577 compound class,IIs
R2004 T3577 T3575 nsubj IIs,are
R2005 T3578 T3579 punct [,5
R2006 T3579 T3561 parataxis 5,suggest
R2007 T3580 T3579 punct ],5
R2008 T3581 T3538 punct .,define
R2009 T3583 T3584 nsubj Figure,illustrates
R2010 T3585 T3583 nummod 1,Figure
R2011 T3586 T3587 det the,diversity
R2012 T3587 T3584 dobj diversity,illustrates
R2013 T3588 T3587 amod phylogenetic,diversity
R2014 T3589 T3587 prep of,diversity
R2015 T3590 T3591 poss our,collection
R2016 T3591 T3589 pobj collection,of
R2017 T3592 T3591 compound cDNA,collection
R2018 T3593 T3584 punct ", ",illustrates
R2019 T3594 T3584 advcl showing,illustrates
R2020 T3595 T3596 mark that,confirmed
R2021 T3596 T3594 ccomp confirmed,showing
R2022 T3597 T3596 nsubj we,confirmed
R2023 T3598 T3596 aux have,confirmed
R2024 T3599 T3596 dobj expression,confirmed
R2025 T3600 T3599 prep of,expression
R2026 T3601 T3602 advmod at,one
R2027 T3602 T3604 nummod one,gene
R2028 T3603 T3602 advmod least,one
R2029 T3604 T3600 pobj gene,of
R2030 T3605 T3606 amod olfactory,receptor
R2031 T3606 T3604 compound receptor,gene
R2032 T3607 T3599 prep in,expression
R2033 T3608 T3609 det each,clade
R2034 T3609 T3607 pobj clade,in
R2035 T3610 T3609 amod major,clade
R2036 T3611 T3609 prep of,clade
R2037 T3612 T3613 det the,genes
R2038 T3613 T3611 pobj genes,of
R2039 T3614 T3613 nmod class,genes
R2040 T3615 T3614 nummod II,class
R2041 T3616 T3617 amod olfactory,receptor
R2042 T3617 T3613 compound receptor,genes
R2043 T3618 T3599 punct ", ",expression
R2044 T3619 T3599 cc or,expression
R2045 T3620 T3621 quantmod 391,983
R2046 T3621 T3599 conj 983,expression
R2047 T3622 T3621 quantmod out,983
R2048 T3623 T3621 quantmod of,983
R2049 T3624 T3625 punct (,%
R2050 T3625 T3621 parataxis %,983
R2051 T3626 T3625 nummod 40,%
R2052 T3627 T3625 punct ),%
R2053 T3628 T3621 prep of,983
R2054 T3629 T3630 det all,genes
R2055 T3630 T3628 pobj genes,of
R2056 T3631 T3630 amod intact,genes
R2057 T3632 T3630 nmod class,genes
R2058 T3633 T3632 nummod II,class
R2059 T3634 T3635 amod olfactory,receptor
R2060 T3635 T3630 compound receptor,genes
R2061 T3636 T3637 advmod where,are
R2062 T3637 T3630 relcl are,genes
R2063 T3638 T3639 amod full,length
R2064 T3639 T3641 nmod length,data
R2065 T3640 T3639 punct -,length
R2066 T3641 T3637 nsubj data,are
R2067 T3642 T3643 amod genomic,sequence
R2068 T3643 T3641 compound sequence,data
R2069 T3644 T3637 acomp available,are
R2070 T3645 T3646 punct (,branches
R2071 T3646 T3596 parataxis branches,confirmed
R2072 T3647 T3646 amod blue,branches
R2073 T3648 T3646 punct ),branches
R2074 T3649 T3584 punct .,illustrates
R2075 T3651 T3652 det The,screen
R2076 T3652 T3653 nsubj screen,appears
R2077 T3654 T3653 advmod thus,appears
R2078 T3655 T3656 advmod relatively,unbiased
R2079 T3656 T3653 oprd unbiased,appears
R2080 T3657 T3653 prep in,appears
R2081 T3658 T3659 poss its,coverage
R2082 T3659 T3657 pobj coverage,in
R2083 T3660 T3659 prep of,coverage
R2084 T3661 T3662 nmod class,receptors
R2085 T3662 T3660 pobj receptors,of
R2086 T3663 T3661 nummod II,class
R2087 T3664 T3662 amod olfactory,receptors
R2088 T3665 T3653 punct .,appears
R2089 T3667 T3668 advmod However,provided
R2090 T3669 T3668 punct ", ",provided
R2091 T3670 T3671 poss our,screen
R2092 T3671 T3668 nsubj screen,provided
R2093 T3672 T3671 amod random,screen
R2094 T3673 T3668 dobj cDNAs,provided
R2095 T3674 T3668 prep for,provided
R2096 T3675 T3676 advmod only,124
R2097 T3676 T3680 nummod 124,receptors
R2098 T3677 T3676 quantmod two,124
R2099 T3678 T3676 quantmod out,124
R2100 T3679 T3676 quantmod of,124
R2101 T3680 T3674 pobj receptors,for
R2102 T3681 T3680 amod intact,receptors
R2103 T3682 T3680 punct ", ",receptors
R2104 T3683 T3684 amod full,length
R2105 T3684 T3680 nmod length,receptors
R2106 T3685 T3684 punct -,length
R2107 T3686 T3680 nmod class,receptors
R2108 T3687 T3686 nummod I,class
R2109 T3688 T3680 amod olfactory,receptors
R2110 T3689 T3668 punct .,provided
R2111 T3691 T3692 prep In,used
R2112 T3693 T3694 det an,attempt
R2113 T3694 T3691 pobj attempt,In
R2114 T3695 T3696 aux to,broaden
R2115 T3696 T3694 acl broaden,attempt
R2116 T3697 T3698 det the,coverage
R2117 T3698 T3696 dobj coverage,broaden
R2118 T3699 T3698 amod phylogenetic,coverage
R2119 T3700 T3698 prep of,coverage
R2120 T3701 T3702 poss our,screen
R2121 T3702 T3700 pobj screen,of
R2122 T3703 T3702 compound hybridization,screen
R2123 T3704 T3692 punct ", ",used
R2124 T3705 T3692 nsubj we,used
R2125 T3706 T3707 amod additional,probes
R2126 T3707 T3692 dobj probes,used
R2127 T3708 T3707 amod degenerate,probes
R2128 T3709 T3692 prep on,used
R2129 T3710 T3711 det the,library
R2130 T3711 T3709 pobj library,on
R2131 T3712 T3711 amod adult,library
R2132 T3713 T3692 cc and,used
R2133 T3714 T3692 conj screened,used
R2134 T3715 T3716 det an,library
R2135 T3716 T3714 dobj library,screened
R2136 T3717 T3716 amod embryonic,library
R2137 T3718 T3719 punct (,Table
R2138 T3719 T3714 parataxis Table,screened
R2139 T3720 T3719 nummod 2,Table
R2140 T3721 T3719 punct ),Table
R2141 T3722 T3692 punct .,used
R2142 T3724 T3725 det These,experiments
R2143 T3725 T3726 nsubj experiments,increase
R2144 T3727 T3726 aux did,increase
R2145 T3728 T3726 neg not,increase
R2146 T3729 T3730 det the,diversity
R2147 T3730 T3726 dobj diversity,increase
R2148 T3731 T3730 prep of,diversity
R2149 T3732 T3731 pobj clones,of
R2150 T3733 T3732 acl identified,clones
R2151 T3734 T3735 punct (,shown
R2152 T3735 T3726 parataxis shown,increase
R2153 T3736 T3735 neg not,shown
R2154 T3737 T3735 punct ),shown
R2155 T3738 T3726 punct .,increase
R2156 T3740 T3741 det This,underrepresentation
R2157 T3741 T3745 nsubj underrepresentation,be
R2158 T3742 T3741 amod severe,underrepresentation
R2159 T3743 T3741 nmod class,underrepresentation
R2160 T3744 T3743 nummod I,class
R2161 T3746 T3745 aux could,be
R2162 T3747 T3745 prep due,be
R2163 T3748 T3747 pcomp to,due
R2164 T3749 T3750 amod experimental,bias
R2165 T3750 T3747 pobj bias,due
R2166 T3751 T3750 punct -,bias
R2167 T3752 T3753 det a,consequence
R2168 T3753 T3750 appos consequence,bias
R2169 T3754 T3753 prep of,consequence
R2170 T3755 T3754 pcomp using,of
R2171 T3756 T3757 amod degenerate,primers
R2172 T3757 T3755 dobj primers,using
R2173 T3758 T3759 aux to,create
R2174 T3759 T3755 advcl create,using
R2175 T3760 T3761 poss our,probe
R2176 T3761 T3759 dobj probe,create
R2177 T3762 T3761 compound hybridization,probe
R2178 T3763 T3745 punct .,be
R2179 T3765 T3766 advmod Alternatively,expressed
R2180 T3767 T3766 punct ", ",expressed
R2181 T3768 T3769 nmod class,genes
R2182 T3769 T3766 nsubjpass genes,expressed
R2183 T3770 T3768 nummod I,class
R2184 T3771 T3766 aux might,expressed
R2185 T3772 T3766 auxpass be,expressed
R2186 T3773 T3766 prep at,expressed
R2187 T3774 T3775 advmod extremely,low
R2188 T3775 T3776 amod low,levels
R2189 T3776 T3773 pobj levels,at
R2190 T3777 T3766 prep in,expressed
R2191 T3778 T3779 det the,epithelium
R2192 T3779 T3777 pobj epithelium,in
R2193 T3780 T3779 amod olfactory,epithelium
R2194 T3781 T3766 punct .,expressed
R2195 T3783 T3784 prep In,designed
R2196 T3785 T3783 pobj order,In
R2197 T3786 T3787 aux to,determine
R2198 T3787 T3785 acl determine,order
R2199 T3788 T3789 mark whether,expressed
R2200 T3789 T3787 ccomp expressed,determine
R2201 T3790 T3791 nmod class,receptors
R2202 T3791 T3789 nsubjpass receptors,expressed
R2203 T3792 T3790 nummod I,class
R2204 T3793 T3791 amod olfactory,receptors
R2205 T3794 T3789 auxpass are,expressed
R2206 T3795 T3789 prep in,expressed
R2207 T3796 T3797 det the,epithelium
R2208 T3797 T3795 pobj epithelium,in
R2209 T3798 T3797 amod olfactory,epithelium
R2210 T3799 T3784 punct ", ",designed
R2211 T3800 T3784 nsubj we,designed
R2212 T3801 T3802 det a,primer
R2213 T3802 T3784 dobj primer,designed
R2214 T3803 T3804 amod reverse,strand
R2215 T3804 T3802 nmod strand,primer
R2216 T3805 T3804 punct -,strand
R2217 T3806 T3802 amod degenerate,primer
R2218 T3807 T3808 aux to,recognize
R2219 T3808 T3784 advcl recognize,designed
R2220 T3809 T3810 det a,motif
R2221 T3810 T3808 dobj motif,recognize
R2222 T3811 T3810 prep in,motif
R2223 T3812 T3813 compound transmembrane,domain
R2224 T3813 T3811 pobj domain,in
R2225 T3814 T3813 nummod 7,domain
R2226 T3815 T3816 punct (,NP
R2227 T3816 T3813 parataxis NP,domain
R2228 T3817 T3816 dep PP,NP
R2229 T3818 T3816 punct {,NP
R2230 T3819 T3820 compound V,T
R2231 T3820 T3816 dep T,NP
R2232 T3821 T3820 punct /,T
R2233 T3822 T3820 compound M,T
R2234 T3823 T3820 punct /,T
R2235 T3824 T3820 compound A,T
R2236 T3825 T3820 punct /,T
R2237 T3826 T3816 advmod },NP
R2238 T3827 T3816 punct {,NP
R2239 T3828 T3829 compound F,M
R2240 T3829 T3816 dep M,NP
R2241 T3830 T3829 punct /,M
R2242 T3831 T3829 compound L,M
R2243 T3832 T3829 punct /,M
R2244 T3833 T3829 compound I,M
R2245 T3834 T3829 punct /,M
R2246 T3835 T3816 advmod },NP
R2247 T3836 T3816 punct ),NP
R2248 T3837 T3810 acl enriched,motif
R2249 T3838 T3837 prep among,enriched
R2250 T3839 T3840 nmod class,sequences
R2251 T3840 T3838 pobj sequences,among
R2252 T3841 T3839 nummod I,class
R2253 T3842 T3843 amod olfactory,receptor
R2254 T3843 T3840 compound receptor,sequences
R2255 T3844 T3784 punct .,designed
R2256 T3846 T3847 nsubjpass Most,shared
R2257 T3848 T3846 prep of,Most
R2258 T3849 T3850 det the,motif
R2259 T3850 T3848 pobj motif,of
R2260 T3851 T3847 auxpass is,shared
R2261 T3852 T3847 prep among,shared
R2262 T3853 T3854 det all,receptors
R2263 T3854 T3852 pobj receptors,among
R2264 T3855 T3854 amod olfactory,receptors
R2265 T3856 T3847 punct ", ",shared
R2266 T3857 T3847 cc but,shared
R2267 T3858 T3859 det the,residue
R2268 T3859 T3862 nsubjpass residue,found
R2269 T3860 T3859 amod first,residue
R2270 T3861 T3859 compound proline,residue
R2271 T3862 T3847 conj found,shared
R2272 T3863 T3859 punct (,residue
R2273 T3864 T3859 prep at,residue
R2274 T3865 T3866 det the,primer
R2275 T3866 T3867 poss primer,end
R2276 T3867 T3864 pobj end,at
R2277 T3868 T3866 case 's,primer
R2278 T3869 T3867 nummod 3,end
R2279 T3870 T3869 punct ',3
R2280 T3871 T3862 punct ),found
R2281 T3872 T3862 auxpass is,found
R2282 T3873 T3862 prep in,found
R2283 T3874 T3875 quantmod 121,124
R2284 T3875 T3878 nummod 124,genes
R2285 T3876 T3875 quantmod out,124
R2286 T3877 T3875 quantmod of,124
R2287 T3878 T3873 pobj genes,in
R2288 T3879 T3880 punct (,%
R2289 T3880 T3875 parataxis %,124
R2290 T3881 T3880 nummod 98,%
R2291 T3882 T3880 punct ),%
R2292 T3883 T3878 amod intact,genes
R2293 T3884 T3878 nmod class,genes
R2294 T3885 T3884 nummod I,class
R2295 T3886 T3862 prep compared,found
R2296 T3887 T3886 prep to,compared
R2297 T3888 T3889 advmod only,983
R2298 T3889 T3893 nummod 983,genes
R2299 T3890 T3889 quantmod 37,983
R2300 T3891 T3889 quantmod out,983
R2301 T3892 T3889 quantmod of,983
R2302 T3893 T3887 pobj genes,to
R2303 T3894 T3895 punct (,%
R2304 T3895 T3889 parataxis %,983
R2305 T3896 T3895 nummod 4,%
R2306 T3897 T3895 punct ),%
R2307 T3898 T3893 amod intact,genes
R2308 T3899 T3893 nmod class,genes
R2309 T3900 T3899 nummod II,class
R2310 T3901 T3862 punct .,found
R2311 T3903 T3904 advmod When,combined
R2312 T3904 T3905 advcl combined,amplifies
R2313 T3905 T3921 ccomp amplifies,represented
R2314 T3906 T3904 prep with,combined
R2315 T3907 T3908 det another,primer
R2316 T3908 T3906 pobj primer,with
R2317 T3909 T3910 amod olfactory,receptor
R2318 T3910 T3908 nmod receptor,primer
R2319 T3911 T3908 amod degenerate,primer
R2320 T3912 T3908 punct ", ",primer
R2321 T3913 T3908 appos P26,primer
R2322 T3914 T3915 punct [,17
R2323 T3915 T3904 parataxis 17,combined
R2324 T3916 T3915 punct ],17
R2325 T3917 T3905 punct ", ",amplifies
R2326 T3918 T3919 det this,primer
R2327 T3919 T3905 nsubj primer,amplifies
R2328 T3920 T3905 advmod preferentially,amplifies
R2329 T3922 T3923 nmod class,receptors
R2330 T3923 T3905 dobj receptors,amplifies
R2331 T3924 T3922 nummod I,class
R2332 T3925 T3923 amod olfactory,receptors
R2333 T3926 T3923 prep from,receptors
R2334 T3927 T3928 nmod mouse,DNA
R2335 T3928 T3926 pobj DNA,from
R2336 T3929 T3928 amod genomic,DNA
R2337 T3930 T3921 punct : ,represented
R2338 T3931 T3921 prep of,represented
R2339 T3932 T3933 nummod 33,products
R2340 T3933 T3931 pobj products,of
R2341 T3934 T3933 amod sequenced,products
R2342 T3935 T3933 punct ", ",products
R2343 T3936 T3933 amod cloned,products
R2344 T3937 T3933 compound PCR,products
R2345 T3938 T3921 punct ", ",represented
R2346 T3939 T3921 nsubj 17,represented
R2347 T3940 T3941 nummod seven,receptors
R2348 T3941 T3921 dobj receptors,represented
R2349 T3942 T3941 amod different,receptors
R2350 T3943 T3941 nmod class,receptors
R2351 T3944 T3943 nummod I,class
R2352 T3945 T3941 amod olfactory,receptors
R2353 T3946 T3921 punct ", ",represented
R2354 T3947 T3948 nsubj six,represented
R2355 T3948 T3921 conj represented,represented
R2356 T3949 T3950 nummod three,receptors
R2357 T3950 T3948 dobj receptors,represented
R2358 T3951 T3950 amod different,receptors
R2359 T3952 T3950 nmod class,receptors
R2360 T3953 T3952 nummod II,class
R2361 T3954 T3950 amod olfactory,receptors
R2362 T3955 T3948 punct ", ",represented
R2363 T3956 T3948 cc and,represented
R2364 T3957 T3958 nsubj ten,represented
R2365 T3958 T3948 conj represented,represented
R2366 T3959 T3960 nummod five,contaminants
R2367 T3960 T3958 dobj contaminants,represented
R2368 T3961 T3960 amod different,contaminants
R2369 T3962 T3963 amod non-olfactory,receptor
R2370 T3963 T3960 compound receptor,contaminants
R2371 T3964 T3921 punct .,represented
R2372 T3966 T3967 amod Degenerate,PCR
R2373 T3967 T3968 nsubj PCR,showed
R2374 T3969 T3967 punct ", ",PCR
R2375 T3970 T3967 conj cloning,PCR
R2376 T3971 T3970 punct ", ",cloning
R2377 T3972 T3970 cc and,cloning
R2378 T3973 T3970 conj sequencing,cloning
R2379 T3974 T3973 prep from,sequencing
R2380 T3975 T3976 amod reverse,transcribed
R2381 T3976 T3978 amod transcribed,RNA
R2382 T3977 T3976 punct -,transcribed
R2383 T3978 T3974 pobj RNA,from
R2384 T3979 T3980 amod olfactory,epithelium
R2385 T3980 T3978 compound epithelium,RNA
R2386 T3981 T3982 mark that,expressed
R2387 T3982 T3968 ccomp expressed,showed
R2388 T3983 T3984 advmod at,seven
R2389 T3984 T3986 nummod seven,receptors
R2390 T3985 T3984 advmod least,seven
R2391 T3986 T3982 nsubjpass receptors,expressed
R2392 T3987 T3986 nmod class,receptors
R2393 T3988 T3987 nummod I,class
R2394 T3989 T3986 amod olfactory,receptors
R2395 T3990 T3982 auxpass are,expressed
R2396 T3991 T3982 punct ", ",expressed
R2397 T3992 T3993 advmod as,as
R2398 T3993 T3995 cc as,gene
R2399 T3994 T3993 advmod well,as
R2400 T3995 T3982 dep gene,expressed
R2401 T3996 T3995 nummod one,gene
R2402 T3997 T3995 amod additional,gene
R2403 T3998 T3995 nmod class,gene
R2404 T3999 T3998 nummod II,class
R2405 T4000 T3995 punct (,gene
R2406 T4001 T3995 acl colored,gene
R2407 T4002 T4001 oprd red,colored
R2408 T4003 T4001 prep in,colored
R2409 T4004 T4003 pobj Figure,in
R2410 T4005 T4004 nummod 1,Figure
R2411 T4006 T3995 punct ),gene
R2412 T4007 T3968 punct .,showed
R2413 T4009 T4010 advmod However,obtained
R2414 T4011 T4010 punct ", ",obtained
R2415 T4012 T4013 det no,products
R2416 T4013 T4010 nsubjpass products,obtained
R2417 T4014 T4010 aux could,obtained
R2418 T4015 T4010 auxpass be,obtained
R2419 T4016 T4010 prep from,obtained
R2420 T4017 T4018 det the,adult
R2421 T4018 T4016 pobj adult,from
R2422 T4019 T4018 cc or,adult
R2423 T4020 T4021 det the,fetal
R2424 T4021 T4022 nmod fetal,libraries
R2425 T4022 T4018 conj libraries,adult
R2426 T4023 T4024 amod olfactory,epithelium
R2427 T4024 T4022 compound epithelium,libraries
R2428 T4025 T4022 compound cDNA,libraries
R2429 T4026 T4010 advcl using,obtained
R2430 T4027 T4028 det the,primer
R2431 T4028 T4026 dobj primer,using
R2432 T4029 T4028 nmod class,primer
R2433 T4030 T4029 nummod I,class
R2434 T4031 T4010 punct ", ",obtained
R2435 T4032 T4010 advcl suggesting,obtained
R2436 T4033 T4034 mark that,contain
R2437 T4034 T4032 ccomp contain,suggesting
R2438 T4035 T4036 det the,libraries
R2439 T4036 T4034 nsubj libraries,contain
R2440 T4037 T4038 advmod very,low
R2441 T4038 T4039 amod low,levels
R2442 T4039 T4034 dobj levels,contain
R2443 T4040 T4039 prep of,levels
R2444 T4041 T4042 nmod class,receptors
R2445 T4042 T4040 pobj receptors,of
R2446 T4043 T4041 nummod I,class
R2447 T4044 T4042 amod olfactory,receptors
R2448 T4045 T4010 punct .,obtained
R2449 T4047 T4048 nsubj We,confirmed
R2450 T4049 T4048 advmod also,confirmed
R2451 T4050 T4048 dobj expression,confirmed
R2452 T4051 T4050 prep of,expression
R2453 T4052 T4053 nummod nine,receptors
R2454 T4053 T4051 pobj receptors,of
R2455 T4054 T4053 amod additional,receptors
R2456 T4055 T4053 amod olfactory,receptors
R2457 T4056 T4053 punct (,receptors
R2458 T4057 T4058 nummod three,class
R2459 T4058 T4053 appos class,receptors
R2460 T4059 T4058 nummod I,class
R2461 T4060 T4058 cc and,class
R2462 T4061 T4062 nummod six,class
R2463 T4062 T4058 conj class,class
R2464 T4063 T4062 nummod II,class
R2465 T4064 T4053 punct ", ",receptors
R2466 T4065 T4053 acl colored,receptors
R2467 T4066 T4065 oprd red,colored
R2468 T4067 T4065 prep in,colored
R2469 T4068 T4067 pobj Figure,in
R2470 T4069 T4068 nummod 1,Figure
R2471 T4070 T4053 punct ),receptors
R2472 T4071 T4048 prep from,confirmed
R2473 T4072 T4071 pobj subclades,from
R2474 T4073 T4074 dep that,represented
R2475 T4074 T4072 relcl represented,subclades
R2476 T4075 T4074 auxpass were,represented
R2477 T4076 T4074 advmod poorly,represented
R2478 T4077 T4074 prep in,represented
R2479 T4078 T4079 poss our,screen
R2480 T4079 T4077 pobj screen,in
R2481 T4080 T4079 compound cDNA,screen
R2482 T4081 T4048 advcl using,confirmed
R2483 T4082 T4083 npadvmod gene,specific
R2484 T4083 T4085 amod specific,pairs
R2485 T4084 T4083 punct -,specific
R2486 T4085 T4081 dobj pairs,using
R2487 T4086 T4085 compound primer,pairs
R2488 T4087 T4088 aux to,amplify
R2489 T4088 T4081 advcl amplify,using
R2490 T4089 T4090 compound cDNA,library
R2491 T4090 T4088 dobj library,amplify
R2492 T4091 T4090 cc or,library
R2493 T4092 T4093 amod reverse,transcribed
R2494 T4093 T4095 amod transcribed,templates
R2495 T4094 T4093 punct -,transcribed
R2496 T4095 T4090 conj templates,library
R2497 T4096 T4095 compound RNA,templates
R2498 T4097 T4048 punct .,confirmed
R2499 T4099 T4100 prep For,determined
R2500 T4101 T4099 pobj two,For
R2501 T4102 T4101 prep of,two
R2502 T4103 T4104 det the,genes
R2503 T4104 T4102 pobj genes,of
R2504 T4105 T4104 nmod class,genes
R2505 T4106 T4105 nummod I,class
R2506 T4107 T4108 nsubj we,shown
R2507 T4108 T4104 advcl shown,genes
R2508 T4109 T4108 aux had,shown
R2509 T4110 T4111 aux to,expressed
R2510 T4111 T4108 xcomp expressed,shown
R2511 T4112 T4111 auxpass be,expressed
R2512 T4113 T4100 punct ", ",determined
R2513 T4114 T4100 nsubj we,determined
R2514 T4115 T4116 amod relative,levels
R2515 T4116 T4100 dobj levels,determined
R2516 T4117 T4116 compound transcript,levels
R2517 T4118 T4100 advcl using,determined
R2518 T4119 T4120 amod quantitative,PCR
R2519 T4120 T4118 dobj PCR,using
R2520 T4121 T4120 compound RT,PCR
R2521 T4122 T4120 punct -,PCR
R2522 T4123 T4124 punct (,see
R2523 T4124 T4100 parataxis see,determined
R2524 T4125 T4124 advmod below,see
R2525 T4126 T4124 punct ),see
R2526 T4127 T4100 punct .,determined
R2527 T4129 T4130 compound Expression,levels
R2528 T4130 T4131 nsubj levels,were
R2529 T4132 T4131 acomp similar,were
R2530 T4133 T4132 prep to,similar
R2531 T4134 T4133 pobj those,to
R2532 T4135 T4134 acl observed,those
R2533 T4136 T4135 prep for,observed
R2534 T4137 T4136 pobj genes,for
R2535 T4138 T4139 dep that,represented
R2536 T4139 T4137 relcl represented,genes
R2537 T4140 T4139 auxpass were,represented
R2538 T4141 T4139 prep in,represented
R2539 T4142 T4143 poss our,collection
R2540 T4143 T4141 pobj collection,in
R2541 T4144 T4143 compound cDNA,collection
R2542 T4145 T4131 punct ", ",were
R2543 T4146 T4131 advcl suggesting,were
R2544 T4147 T4148 mark that,represented
R2545 T4148 T4146 advcl represented,suggesting
R2546 T4149 T4150 nmod class,receptors
R2547 T4150 T4148 nsubjpass receptors,represented
R2548 T4151 T4149 nummod I,class
R2549 T4152 T4150 amod olfactory,receptors
R2550 T4153 T4148 auxpass are,represented
R2551 T4154 T4148 neg not,represented
R2552 T4155 T4148 advmod under,represented
R2553 T4156 T4148 punct -,represented
R2554 T4157 T4148 prep in,represented
R2555 T4158 T4159 det the,epithelium
R2556 T4159 T4157 pobj epithelium,in
R2557 T4160 T4159 amod olfactory,epithelium
R2558 T4161 T4148 punct ", ",represented
R2559 T4162 T4148 cc and,represented
R2560 T4163 T4164 mark that,is
R2561 T4164 T4148 conj is,represented
R2562 T4165 T4166 det the,dearth
R2563 T4166 T4164 nsubj dearth,is
R2564 T4167 T4166 prep of,dearth
R2565 T4168 T4169 nmod class,cDNAs
R2566 T4169 T4167 pobj cDNAs,of
R2567 T4170 T4168 nummod I,class
R2568 T4171 T4166 prep in,dearth
R2569 T4172 T4173 poss our,screen
R2570 T4173 T4171 pobj screen,in
R2571 T4174 T4164 acomp likely,is
R2572 T4175 T4176 aux to,be
R2573 T4176 T4174 xcomp be,likely
R2574 T4177 T4176 prep due,be
R2575 T4178 T4177 pcomp to,due
R2576 T4179 T4177 pobj bias,due
R2577 T4180 T4179 prep in,bias
R2578 T4181 T4182 det the,libraries
R2579 T4182 T4180 pobj libraries,in
R2580 T4183 T4182 cc and,libraries
R2581 T4184 T4183 punct /,and
R2582 T4185 T4183 cc or,and
R2583 T4186 T4187 compound hybridization,probes
R2584 T4187 T4182 conj probes,libraries
R2585 T4188 T4131 punct .,were