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PMC:314463 / 56161-57785 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T12097 0-10 JJ denotes Phenotypic
T12098 11-19 NN denotes analysis
T12099 19-211 sentence denotes For measurements of hair length and color, the entire interlimb region of skin was first dissected with a single incision at the dorsal midline and preserved with powdered sodium bicarbonate.
T12100 20-23 IN denotes For
T12102 24-36 NNS denotes measurements
T12103 37-39 IN denotes of
T12104 40-44 NN denotes hair
T12105 45-51 NN denotes length
T12106 52-55 CC denotes and
T12107 56-61 NN denotes color
T12108 61-63 , denotes ,
T12109 63-66 DT denotes the
T12111 67-73 JJ denotes entire
T12112 74-83 NN denotes interlimb
T12110 84-90 NN denotes region
T12113 91-93 IN denotes of
T12114 94-98 NN denotes skin
T12115 99-102 VBD denotes was
T12116 103-108 RB denotes first
T12101 109-118 VBN denotes dissected
T12117 119-123 IN denotes with
T12118 124-125 DT denotes a
T12120 126-132 JJ denotes single
T12119 133-141 NN denotes incision
T12121 142-144 IN denotes at
T12122 145-148 DT denotes the
T12124 149-155 JJ denotes dorsal
T12123 156-163 NN denotes midline
T12125 164-167 CC denotes and
T12126 168-177 VBN denotes preserved
T12127 178-182 IN denotes with
T12128 183-191 VBN denotes powdered
T12130 192-198 NN denotes sodium
T12129 199-210 NN denotes bicarbonate
T12131 210-211 . denotes .
T12132 211-479 sentence denotes Slices 2–2.5 mm in width were then prepared parallel to the dorsoventral axis, hair length boundaries determined from electronic images with Adobe Photoshop (San Jose, California, United States), and measurements obtained using ImageJ (National Institutes of Health).
T12133 212-218 NNS denotes Slices
T12135 219-220 CD denotes 2
T12137 220-221 HYPH denotes –
T12136 221-224 CD denotes 2.5
T12138 225-227 NN denotes mm
T12139 228-230 IN denotes in
T12140 231-236 NN denotes width
T12141 237-241 VBD denotes were
T12142 242-246 RB denotes then
T12134 247-255 VBN denotes prepared
T12143 256-264 RB denotes parallel
T12144 265-267 IN denotes to
T12145 268-271 DT denotes the
T12147 272-284 JJ denotes dorsoventral
T12146 285-289 NN denotes axis
T12148 289-291 , denotes ,
T12149 291-295 NN denotes hair
T12151 296-302 NN denotes length
T12150 303-313 NNS denotes boundaries
T12152 314-324 VBN denotes determined
T12153 325-329 IN denotes from
T12154 330-340 JJ denotes electronic
T12155 341-347 NNS denotes images
T12156 348-352 IN denotes with
T12157 353-358 NNP denotes Adobe
T12158 359-368 NNP denotes Photoshop
T12159 369-370 -LRB- denotes (
T12161 370-373 NNP denotes San
T12160 374-378 NNP denotes Jose
T12162 378-380 , denotes ,
T12163 380-390 NNP denotes California
T12164 390-392 , denotes ,
T12165 392-398 NNP denotes United
T12166 399-405 NNP denotes States
T12167 405-406 -RRB- denotes )
T12168 406-408 , denotes ,
T12169 408-411 CC denotes and
T12170 412-424 NNS denotes measurements
T12171 425-433 VBN denotes obtained
T12172 434-439 VBG denotes using
T12173 440-446 NNP denotes ImageJ
T12174 447-448 -LRB- denotes (
T12176 448-456 NNP denotes National
T12175 457-467 NNPS denotes Institutes
T12177 468-470 IN denotes of
T12178 471-477 NNP denotes Health
T12179 477-478 -RRB- denotes )
T12180 478-479 . denotes .
T12181 479-614 sentence denotes This approach samples awls and auchenes, because they are much thicker and therefore visually more predominant than zigzag underhairs.
T12182 480-484 DT denotes This
T12183 485-493 NN denotes approach
T12184 494-501 VBZ denotes samples
T12185 502-506 NNS denotes awls
T12186 507-510 CC denotes and
T12187 511-519 NNS denotes auchenes
T12188 519-521 , denotes ,
T12189 521-528 IN denotes because
T12191 529-533 PRP denotes they
T12190 534-537 VBP denotes are
T12192 538-542 RB denotes much
T12193 543-550 JJR denotes thicker
T12194 551-554 CC denotes and
T12195 555-564 RB denotes therefore
T12197 565-573 RB denotes visually
T12198 574-578 RBR denotes more
T12196 579-590 JJ denotes predominant
T12199 591-595 IN denotes than
T12200 596-602 NN denotes zigzag
T12201 603-613 NNS denotes underhairs
T12202 613-614 . denotes .
T12203 614-837 sentence denotes To assess dorsoventral variation in hair-type distribution, several hundred hairs were plucked from the middorsum or midventrum of 8-wk-old male BA strain animals, then sorted and categorized using a dissection microscope.
T12204 615-617 TO denotes To
T12205 618-624 VB denotes assess
T12207 625-637 JJ denotes dorsoventral
T12208 638-647 NN denotes variation
T12209 648-650 IN denotes in
T12210 651-655 NN denotes hair
T12212 655-656 HYPH denotes -
T12211 656-660 NN denotes type
T12213 661-673 NN denotes distribution
T12214 673-675 , denotes ,
T12215 675-682 JJ denotes several
T12217 683-690 CD denotes hundred
T12216 691-696 NNS denotes hairs
T12218 697-701 VBD denotes were
T12206 702-709 VBN denotes plucked
T12219 710-714 IN denotes from
T12220 715-718 DT denotes the
T12221 719-728 NN denotes middorsum
T12222 729-731 CC denotes or
T12223 732-742 NN denotes midventrum
T12224 743-745 IN denotes of
T12225 746-747 CD denotes 8
T12227 747-748 HYPH denotes -
T12226 748-750 NN denotes wk
T12229 750-751 HYPH denotes -
T12228 751-754 JJ denotes old
T12231 755-759 JJ denotes male
T12232 760-762 NN denotes BA
T12233 763-769 NN denotes strain
T12230 770-777 NNS denotes animals
T12234 777-779 , denotes ,
T12235 779-783 RB denotes then
T12236 784-790 VBN denotes sorted
T12237 791-794 CC denotes and
T12238 795-806 VBN denotes categorized
T12239 807-812 VBG denotes using
T12240 813-814 DT denotes a
T12242 815-825 NN denotes dissection
T12241 826-836 NN denotes microscope
T12243 836-837 . denotes .
T12244 837-899 sentence denotes No attempt was made to distinguish between awls and auchenes.
T12245 838-840 DT denotes No
T12246 841-848 NN denotes attempt
T12248 849-852 VBD denotes was
T12247 853-857 VBN denotes made
T12249 858-860 TO denotes to
T12250 861-872 VB denotes distinguish
T12251 873-880 IN denotes between
T12252 881-885 NNS denotes awls
T12253 886-889 CC denotes and
T12254 890-898 NNS denotes auchenes
T12255 898-899 . denotes .
T12256 899-1005 sentence denotes For skin histology, 12 μm sections from paraffin-embedded tissue were stained with hematoxylin and eosin.
T12257 900-903 IN denotes For
T12259 904-908 NN denotes skin
T12260 909-918 NN denotes histology
T12261 918-920 , denotes ,
T12262 920-922 CD denotes 12
T12263 923-925 NN denotes μm
T12264 926-934 NNS denotes sections
T12265 935-939 IN denotes from
T12266 940-948 NN denotes paraffin
T12268 948-949 HYPH denotes -
T12267 949-957 VBN denotes embedded
T12269 958-964 NN denotes tissue
T12270 965-969 VBD denotes were
T12258 970-977 VBN denotes stained
T12271 978-982 IN denotes with
T12272 983-994 NN denotes hematoxylin
T12273 995-998 CC denotes and
T12274 999-1004 NN denotes eosin
T12275 1004-1005 . denotes .
T12276 1005-1411 sentence denotes For DOPA staining, the dermis and epidermis were split after 3 h of incubation in 2 M sodium bromide at 37°C (this preparation causes most hair follicles to remain with the dermis), individually fixed for 1 h, then rinsed and stained with 0.1% L-DOPA (Sigma, St. Louis, Missouri, United States), 0.1 M sodium phosphate buffer (pH 6.8) for 5 h at 37°C in the dark, changing the staining solution after 1 h.
T12277 1006-1009 IN denotes For
T12279 1010-1014 NN denotes DOPA
T12280 1015-1023 NN denotes staining
T12281 1023-1025 , denotes ,
T12282 1025-1028 DT denotes the
T12283 1029-1035 NN denotes dermis
T12284 1036-1039 CC denotes and
T12285 1040-1049 NN denotes epidermis
T12286 1050-1054 VBD denotes were
T12278 1055-1060 VBN denotes split
T12287 1061-1066 IN denotes after
T12288 1067-1068 CD denotes 3
T12289 1069-1070 NN denotes h
T12290 1071-1073 IN denotes of
T12291 1074-1084 NN denotes incubation
T12292 1085-1087 IN denotes in
T12293 1088-1089 CD denotes 2
T12294 1090-1091 NN denotes M
T12296 1092-1098 NN denotes sodium
T12295 1099-1106 NN denotes bromide
T12297 1107-1109 IN denotes at
T12298 1110-1112 CD denotes 37
T12299 1112-1114 NN denotes °C
T12300 1115-1116 -LRB- denotes (
T12302 1116-1120 DT denotes this
T12303 1121-1132 NN denotes preparation
T12301 1133-1139 VBZ denotes causes
T12304 1140-1144 JJS denotes most
T12306 1145-1149 NN denotes hair
T12305 1150-1159 NNS denotes follicles
T12308 1160-1162 TO denotes to
T12307 1163-1169 VB denotes remain
T12309 1170-1174 IN denotes with
T12310 1175-1178 DT denotes the
T12311 1179-1185 NN denotes dermis
T12312 1185-1186 -RRB- denotes )
T12313 1186-1188 , denotes ,
T12314 1188-1200 RB denotes individually
T12315 1201-1206 VBN denotes fixed
T12316 1207-1210 IN denotes for
T12317 1211-1212 CD denotes 1
T12318 1213-1214 NN denotes h
T12319 1214-1216 , denotes ,
T12320 1216-1220 RB denotes then
T12321 1221-1227 VBD denotes rinsed
T12322 1228-1231 CC denotes and
T12323 1232-1239 VBD denotes stained
T12324 1240-1244 IN denotes with
T12325 1245-1248 CD denotes 0.1
T12326 1248-1249 NN denotes %
T12328 1250-1251 NN denotes L
T12329 1251-1252 HYPH denotes -
T12327 1252-1256 NN denotes DOPA
T12331 1257-1258 -LRB- denotes (
T12332 1258-1263 NNP denotes Sigma
T12333 1263-1265 , denotes ,
T12334 1265-1268 NNP denotes St.
T12335 1269-1274 NNP denotes Louis
T12336 1274-1276 , denotes ,
T12337 1276-1284 NNP denotes Missouri
T12338 1284-1286 , denotes ,
T12339 1286-1292 NNP denotes United
T12340 1293-1299 NNP denotes States
T12341 1299-1300 -RRB- denotes )
T12342 1300-1302 , denotes ,
T12343 1302-1305 CD denotes 0.1
T12344 1306-1307 NN denotes M
T12346 1308-1314 NN denotes sodium
T12345 1315-1324 NN denotes phosphate
T12330 1325-1331 NN denotes buffer
T12347 1332-1333 -LRB- denotes (
T12348 1333-1335 NN denotes pH
T12349 1336-1339 CD denotes 6.8
T12350 1339-1340 -RRB- denotes )
T12351 1341-1344 IN denotes for
T12352 1345-1346 CD denotes 5
T12353 1347-1348 NN denotes h
T12354 1349-1351 IN denotes at
T12355 1352-1354 CD denotes 37
T12356 1354-1356 NN denotes °C
T12357 1357-1359 IN denotes in
T12358 1360-1363 DT denotes the
T12359 1364-1368 NN denotes dark
T12360 1368-1370 , denotes ,
T12361 1370-1378 VBG denotes changing
T12362 1379-1382 DT denotes the
T12364 1383-1391 NN denotes staining
T12363 1392-1400 NN denotes solution
T12365 1401-1406 IN denotes after
T12366 1407-1408 CD denotes 1
T12367 1409-1410 NN denotes h
T12368 1410-1411 . denotes .
T12369 1411-1475 sentence denotes The samples were then fixed overnight, dehydrated, and mounted.
T12370 1412-1415 DT denotes The
T12371 1416-1423 NNS denotes samples
T12373 1424-1428 VBD denotes were
T12374 1429-1433 RB denotes then
T12372 1434-1439 VBN denotes fixed
T12375 1440-1449 RB denotes overnight
T12376 1449-1451 , denotes ,
T12377 1451-1461 VBN denotes dehydrated
T12378 1461-1463 , denotes ,
T12379 1463-1466 CC denotes and
T12380 1467-1474 VBN denotes mounted
T12381 1474-1475 . denotes .
T12382 1475-1574 sentence denotes This method is sufficient to stain interfollicular melanocytes without creating a high background.
T12383 1476-1480 DT denotes This
T12384 1481-1487 NN denotes method
T12385 1488-1490 VBZ denotes is
T12386 1491-1501 JJ denotes sufficient
T12387 1502-1504 TO denotes to
T12388 1505-1510 VB denotes stain
T12389 1511-1526 JJ denotes interfollicular
T12390 1527-1538 NNS denotes melanocytes
T12391 1539-1546 IN denotes without
T12392 1547-1555 VBG denotes creating
T12393 1556-1557 DT denotes a
T12395 1558-1562 JJ denotes high
T12394 1563-1573 NN denotes background
T12396 1573-1574 . denotes .
T12397 1574-1624 sentence denotes The fixative used was always 4% paraformaldehyde.
T12398 1575-1578 DT denotes The
T12399 1579-1587 JJ denotes fixative
T12401 1588-1592 VBN denotes used
T12400 1593-1596 VBD denotes was
T12402 1597-1603 RB denotes always
T12403 1604-1605 CD denotes 4
T12404 1605-1606 NN denotes %
T12405 1607-1623 NN denotes paraformaldehyde
T12406 1623-1624 . denotes .
R8429 T12097 T12098 amod Phenotypic,analysis
R8430 T12100 T12101 prep For,dissected
R8431 T12102 T12100 pobj measurements,For
R8432 T12103 T12102 prep of,measurements
R8433 T12104 T12105 compound hair,length
R8434 T12105 T12103 pobj length,of
R8435 T12106 T12105 cc and,length
R8436 T12107 T12105 conj color,length
R8437 T12108 T12101 punct ", ",dissected
R8438 T12109 T12110 det the,region
R8439 T12110 T12101 nsubjpass region,dissected
R8440 T12111 T12110 amod entire,region
R8441 T12112 T12110 compound interlimb,region
R8442 T12113 T12110 prep of,region
R8443 T12114 T12113 pobj skin,of
R8444 T12115 T12101 auxpass was,dissected
R8445 T12116 T12101 advmod first,dissected
R8446 T12117 T12101 prep with,dissected
R8447 T12118 T12119 det a,incision
R8448 T12119 T12117 pobj incision,with
R8449 T12120 T12119 amod single,incision
R8450 T12121 T12119 prep at,incision
R8451 T12122 T12123 det the,midline
R8452 T12123 T12121 pobj midline,at
R8453 T12124 T12123 amod dorsal,midline
R8454 T12125 T12101 cc and,dissected
R8455 T12126 T12101 conj preserved,dissected
R8456 T12127 T12126 prep with,preserved
R8457 T12128 T12129 amod powdered,bicarbonate
R8458 T12129 T12127 pobj bicarbonate,with
R8459 T12130 T12129 compound sodium,bicarbonate
R8460 T12131 T12101 punct .,dissected
R8461 T12133 T12134 nsubjpass Slices,prepared
R8462 T12135 T12136 compound 2,2.5
R8463 T12136 T12138 nummod 2.5,mm
R8464 T12137 T12136 punct –,2.5
R8465 T12138 T12139 nsubj mm,in
R8466 T12139 T12133 relcl in,Slices
R8467 T12140 T12139 pobj width,in
R8468 T12141 T12134 auxpass were,prepared
R8469 T12142 T12134 advmod then,prepared
R8470 T12143 T12134 advmod parallel,prepared
R8471 T12144 T12143 prep to,parallel
R8472 T12145 T12146 det the,axis
R8473 T12146 T12144 pobj axis,to
R8474 T12147 T12146 amod dorsoventral,axis
R8475 T12148 T12134 punct ", ",prepared
R8476 T12149 T12150 compound hair,boundaries
R8477 T12150 T12152 nsubj boundaries,determined
R8478 T12151 T12150 compound length,boundaries
R8479 T12152 T12134 conj determined,prepared
R8480 T12153 T12152 prep from,determined
R8481 T12154 T12155 amod electronic,images
R8482 T12155 T12153 pobj images,from
R8483 T12156 T12152 prep with,determined
R8484 T12157 T12158 compound Adobe,Photoshop
R8485 T12158 T12156 pobj Photoshop,with
R8486 T12159 T12160 punct (,Jose
R8487 T12160 T12158 parataxis Jose,Photoshop
R8488 T12161 T12160 compound San,Jose
R8489 T12162 T12160 punct ", ",Jose
R8490 T12163 T12160 npadvmod California,Jose
R8491 T12164 T12160 punct ", ",Jose
R8492 T12165 T12166 compound United,States
R8493 T12166 T12160 npadvmod States,Jose
R8494 T12167 T12160 punct ),Jose
R8495 T12168 T12152 punct ", ",determined
R8496 T12169 T12152 cc and,determined
R8497 T12170 T12171 nsubj measurements,obtained
R8498 T12171 T12152 conj obtained,determined
R8499 T12172 T12171 advcl using,obtained
R8500 T12173 T12172 dobj ImageJ,using
R8501 T12174 T12175 punct (,Institutes
R8502 T12175 T12173 parataxis Institutes,ImageJ
R8503 T12176 T12175 compound National,Institutes
R8504 T12177 T12175 prep of,Institutes
R8505 T12178 T12177 pobj Health,of
R8506 T12179 T12175 punct ),Institutes
R8507 T12180 T12171 punct .,obtained
R8508 T12182 T12183 det This,approach
R8509 T12183 T12184 nsubj approach,samples
R8510 T12185 T12184 dobj awls,samples
R8511 T12186 T12185 cc and,awls
R8512 T12187 T12185 conj auchenes,awls
R8513 T12188 T12184 punct ", ",samples
R8514 T12189 T12190 mark because,are
R8515 T12190 T12184 advcl are,samples
R8516 T12191 T12190 nsubj they,are
R8517 T12192 T12193 advmod much,thicker
R8518 T12193 T12190 acomp thicker,are
R8519 T12194 T12193 cc and,thicker
R8520 T12195 T12196 advmod therefore,predominant
R8521 T12196 T12193 conj predominant,thicker
R8522 T12197 T12196 advmod visually,predominant
R8523 T12198 T12196 advmod more,predominant
R8524 T12199 T12193 prep than,thicker
R8525 T12200 T12201 compound zigzag,underhairs
R8526 T12201 T12199 pobj underhairs,than
R8527 T12202 T12184 punct .,samples
R8528 T12204 T12205 aux To,assess
R8529 T12205 T12206 advcl assess,plucked
R8530 T12207 T12208 amod dorsoventral,variation
R8531 T12208 T12205 dobj variation,assess
R8532 T12209 T12208 prep in,variation
R8533 T12210 T12211 compound hair,type
R8534 T12211 T12213 compound type,distribution
R8535 T12212 T12211 punct -,type
R8536 T12213 T12209 pobj distribution,in
R8537 T12214 T12206 punct ", ",plucked
R8538 T12215 T12216 amod several,hairs
R8539 T12216 T12206 nsubjpass hairs,plucked
R8540 T12217 T12216 nummod hundred,hairs
R8541 T12218 T12206 auxpass were,plucked
R8542 T12219 T12206 prep from,plucked
R8543 T12220 T12221 det the,middorsum
R8544 T12221 T12219 pobj middorsum,from
R8545 T12222 T12221 cc or,middorsum
R8546 T12223 T12221 conj midventrum,middorsum
R8547 T12224 T12221 prep of,middorsum
R8548 T12225 T12226 nummod 8,wk
R8549 T12226 T12228 npadvmod wk,old
R8550 T12227 T12226 punct -,wk
R8551 T12228 T12230 amod old,animals
R8552 T12229 T12228 punct -,old
R8553 T12230 T12224 pobj animals,of
R8554 T12231 T12230 amod male,animals
R8555 T12232 T12233 compound BA,strain
R8556 T12233 T12230 compound strain,animals
R8557 T12234 T12206 punct ", ",plucked
R8558 T12235 T12236 advmod then,sorted
R8559 T12236 T12206 dep sorted,plucked
R8560 T12237 T12236 cc and,sorted
R8561 T12238 T12236 conj categorized,sorted
R8562 T12239 T12236 advcl using,sorted
R8563 T12240 T12241 det a,microscope
R8564 T12241 T12239 dobj microscope,using
R8565 T12242 T12241 compound dissection,microscope
R8566 T12243 T12206 punct .,plucked
R8567 T12245 T12246 det No,attempt
R8568 T12246 T12247 nsubjpass attempt,made
R8569 T12248 T12247 auxpass was,made
R8570 T12249 T12250 aux to,distinguish
R8571 T12250 T12247 xcomp distinguish,made
R8572 T12251 T12250 prep between,distinguish
R8573 T12252 T12251 pobj awls,between
R8574 T12253 T12252 cc and,awls
R8575 T12254 T12252 conj auchenes,awls
R8576 T12255 T12247 punct .,made
R8577 T12257 T12258 prep For,stained
R8578 T12259 T12260 compound skin,histology
R8579 T12260 T12257 pobj histology,For
R8580 T12261 T12258 punct ", ",stained
R8581 T12262 T12263 nummod 12,μm
R8582 T12263 T12264 compound μm,sections
R8583 T12264 T12258 nsubjpass sections,stained
R8584 T12265 T12264 prep from,sections
R8585 T12266 T12267 npadvmod paraffin,embedded
R8586 T12267 T12269 amod embedded,tissue
R8587 T12268 T12267 punct -,embedded
R8588 T12269 T12265 pobj tissue,from
R8589 T12270 T12258 auxpass were,stained
R8590 T12271 T12258 prep with,stained
R8591 T12272 T12271 pobj hematoxylin,with
R8592 T12273 T12272 cc and,hematoxylin
R8593 T12274 T12272 conj eosin,hematoxylin
R8594 T12275 T12258 punct .,stained
R8595 T12277 T12278 prep For,split
R8596 T12279 T12280 compound DOPA,staining
R8597 T12280 T12277 pobj staining,For
R8598 T12281 T12278 punct ", ",split
R8599 T12282 T12283 det the,dermis
R8600 T12283 T12278 nsubjpass dermis,split
R8601 T12284 T12283 cc and,dermis
R8602 T12285 T12283 conj epidermis,dermis
R8603 T12286 T12278 auxpass were,split
R8604 T12287 T12278 prep after,split
R8605 T12288 T12289 nummod 3,h
R8606 T12289 T12287 pobj h,after
R8607 T12290 T12289 prep of,h
R8608 T12291 T12290 pobj incubation,of
R8609 T12292 T12291 prep in,incubation
R8610 T12293 T12294 nummod 2,M
R8611 T12294 T12295 compound M,bromide
R8612 T12295 T12292 pobj bromide,in
R8613 T12296 T12295 compound sodium,bromide
R8614 T12297 T12291 prep at,incubation
R8615 T12298 T12299 nummod 37,°C
R8616 T12299 T12297 pobj °C,at
R8617 T12300 T12301 punct (,causes
R8618 T12301 T12291 parataxis causes,incubation
R8619 T12302 T12303 det this,preparation
R8620 T12303 T12301 nsubj preparation,causes
R8621 T12304 T12305 amod most,follicles
R8622 T12305 T12307 nsubj follicles,remain
R8623 T12306 T12305 compound hair,follicles
R8624 T12307 T12301 ccomp remain,causes
R8625 T12308 T12307 aux to,remain
R8626 T12309 T12307 prep with,remain
R8627 T12310 T12311 det the,dermis
R8628 T12311 T12309 pobj dermis,with
R8629 T12312 T12301 punct ),causes
R8630 T12313 T12278 punct ", ",split
R8631 T12314 T12315 advmod individually,fixed
R8632 T12315 T12278 dep fixed,split
R8633 T12316 T12315 prep for,fixed
R8634 T12317 T12318 nummod 1,h
R8635 T12318 T12316 pobj h,for
R8636 T12319 T12278 punct ", ",split
R8637 T12320 T12321 advmod then,rinsed
R8638 T12321 T12278 dep rinsed,split
R8639 T12322 T12321 cc and,rinsed
R8640 T12323 T12321 conj stained,rinsed
R8641 T12324 T12323 prep with,stained
R8642 T12325 T12326 nummod 0.1,%
R8643 T12326 T12327 nmod %,DOPA
R8644 T12327 T12330 nmod DOPA,buffer
R8645 T12328 T12327 nmod L,DOPA
R8646 T12329 T12327 punct -,DOPA
R8647 T12330 T12324 pobj buffer,with
R8648 T12331 T12332 punct (,Sigma
R8649 T12332 T12327 parataxis Sigma,DOPA
R8650 T12333 T12332 punct ", ",Sigma
R8651 T12334 T12335 compound St.,Louis
R8652 T12335 T12332 npadvmod Louis,Sigma
R8653 T12336 T12332 punct ", ",Sigma
R8654 T12337 T12332 npadvmod Missouri,Sigma
R8655 T12338 T12332 punct ", ",Sigma
R8656 T12339 T12340 compound United,States
R8657 T12340 T12332 npadvmod States,Sigma
R8658 T12341 T12332 punct ),Sigma
R8659 T12342 T12327 punct ", ",DOPA
R8660 T12343 T12344 nummod 0.1,M
R8661 T12344 T12345 compound M,phosphate
R8662 T12345 T12327 appos phosphate,DOPA
R8663 T12346 T12345 compound sodium,phosphate
R8664 T12347 T12330 punct (,buffer
R8665 T12348 T12330 npadvmod pH,buffer
R8666 T12349 T12348 nummod 6.8,pH
R8667 T12350 T12330 punct ),buffer
R8668 T12351 T12330 prep for,buffer
R8669 T12352 T12353 nummod 5,h
R8670 T12353 T12351 pobj h,for
R8671 T12354 T12330 prep at,buffer
R8672 T12355 T12356 nummod 37,°C
R8673 T12356 T12354 pobj °C,at
R8674 T12357 T12330 prep in,buffer
R8675 T12358 T12359 det the,dark
R8676 T12359 T12357 pobj dark,in
R8677 T12360 T12323 punct ", ",stained
R8678 T12361 T12323 advcl changing,stained
R8679 T12362 T12363 det the,solution
R8680 T12363 T12361 dobj solution,changing
R8681 T12364 T12363 compound staining,solution
R8682 T12365 T12361 prep after,changing
R8683 T12366 T12367 nummod 1,h
R8684 T12367 T12365 pobj h,after
R8685 T12368 T12278 punct .,split
R8686 T12370 T12371 det The,samples
R8687 T12371 T12372 nsubjpass samples,fixed
R8688 T12373 T12372 auxpass were,fixed
R8689 T12374 T12372 advmod then,fixed
R8690 T12375 T12372 advmod overnight,fixed
R8691 T12376 T12372 punct ", ",fixed
R8692 T12377 T12372 conj dehydrated,fixed
R8693 T12378 T12377 punct ", ",dehydrated
R8694 T12379 T12377 cc and,dehydrated
R8695 T12380 T12377 conj mounted,dehydrated
R8696 T12381 T12372 punct .,fixed
R8697 T12383 T12384 det This,method
R8698 T12384 T12385 nsubj method,is
R8699 T12386 T12385 acomp sufficient,is
R8700 T12387 T12388 aux to,stain
R8701 T12388 T12386 xcomp stain,sufficient
R8702 T12389 T12390 amod interfollicular,melanocytes
R8703 T12390 T12388 dobj melanocytes,stain
R8704 T12391 T12388 prep without,stain
R8705 T12392 T12391 pcomp creating,without
R8706 T12393 T12394 det a,background
R8707 T12394 T12392 dobj background,creating
R8708 T12395 T12394 amod high,background
R8709 T12396 T12385 punct .,is
R8710 T12398 T12399 det The,fixative
R8711 T12399 T12400 nsubj fixative,was
R8712 T12401 T12399 acl used,fixative
R8713 T12402 T12400 advmod always,was
R8714 T12403 T12404 nummod 4,%
R8715 T12404 T12405 compound %,paraformaldehyde
R8716 T12405 T12400 attr paraformaldehyde,was
R8717 T12406 T12400 punct .,was