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PMC:194730 / 31250-31825 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T7951 0-13 NN denotes Acetylcholine
T7952 14-22 NN denotes esterase
T7953 23-28 NN denotes assay
T7954 28-213 sentence denotes A 30 μl sample of red blood cell vesicles was mixed with an equal volume of buffer (20 mM Hepes, pH 7.6, 150 mM NaCl, 0.5 % Triton X-100), vortexed and incubated for 5 minutes at 37°C.
T7955 29-30 DT denotes A
T7957 31-33 CD denotes 30
T7958 34-36 NN denotes μl
T7956 37-43 NN denotes sample
T7960 44-46 IN denotes of
T7961 47-50 JJ denotes red
T7963 51-56 NN denotes blood
T7962 57-61 NN denotes cell
T7964 62-70 NNS denotes vesicles
T7965 71-74 VBD denotes was
T7959 75-80 VBN denotes mixed
T7966 81-85 IN denotes with
T7967 86-88 DT denotes an
T7969 89-94 JJ denotes equal
T7968 95-101 NN denotes volume
T7970 102-104 IN denotes of
T7971 105-111 NN denotes buffer
T7972 112-113 -LRB- denotes (
T7974 113-115 CD denotes 20
T7975 116-118 NN denotes mM
T7976 119-124 NN denotes Hepes
T7977 124-126 , denotes ,
T7978 126-128 NN denotes pH
T7979 129-132 CD denotes 7.6
T7980 132-134 , denotes ,
T7981 134-137 CD denotes 150
T7982 138-140 NN denotes mM
T7983 141-145 NN denotes NaCl
T7984 145-147 , denotes ,
T7985 147-150 CD denotes 0.5
T7986 151-152 NN denotes %
T7987 153-159 NN denotes Triton
T7973 160-161 NN denotes X
T7988 161-162 HYPH denotes -
T7989 162-165 CD denotes 100
T7990 165-166 -RRB- denotes )
T7991 166-168 , denotes ,
T7992 168-176 VBN denotes vortexed
T7993 177-180 CC denotes and
T7994 181-190 VBN denotes incubated
T7995 191-194 IN denotes for
T7996 195-196 CD denotes 5
T7997 197-204 NNS denotes minutes
T7998 205-207 IN denotes at
T7999 208-210 CD denotes 37
T8000 210-212 NNS denotes °C
T8001 212-213 . denotes .
T8002 213-433 sentence denotes Subsequently 640 μl sodium phosphate buffer (100 mM Na2PO4, pH 7.6), 50 μl DTNB (5,5'-dithiobis-(2-nitrobenzoic acid), 10 mM in sodium phosphate buffer), and 50 μl acetylthiocholine chloride (12,5 mM in H2O) were added.
T8003 214-226 RB denotes Subsequently
T8005 227-230 CD denotes 640
T8006 231-233 NN denotes μl
T8008 234-240 NN denotes sodium
T8009 241-250 NN denotes phosphate
T8007 251-257 NN denotes buffer
T8010 258-259 -LRB- denotes (
T8012 259-262 CD denotes 100
T8013 263-265 NN denotes mM
T8011 266-272 NN denotes Na2PO4
T8014 272-274 , denotes ,
T8015 274-276 NN denotes pH
T8016 277-280 CD denotes 7.6
T8017 280-281 -RRB- denotes )
T8018 281-283 , denotes ,
T8019 283-285 CD denotes 50
T8020 286-288 NN denotes μl
T8021 289-293 NN denotes DTNB
T8022 294-295 -LRB- denotes (
T8024 295-296 CD denotes 5
T8026 296-297 , denotes ,
T8027 297-298 CD denotes 5
T8028 298-299 SYM denotes '
T8029 299-300 HYPH denotes -
T8025 300-309 NN denotes dithiobis
T8030 309-310 HYPH denotes -
T8031 310-311 -LRB- denotes (
T8033 311-312 CD denotes 2
T8034 312-313 HYPH denotes -
T8035 313-325 JJ denotes nitrobenzoic
T8032 326-330 NN denotes acid
T8036 330-331 -RRB- denotes )
T8037 331-333 , denotes ,
T8038 333-335 CD denotes 10
T8023 336-338 NN denotes mM
T8039 339-341 IN denotes in
T8040 342-348 NN denotes sodium
T8041 349-358 NN denotes phosphate
T8042 359-365 NN denotes buffer
T8043 365-366 -RRB- denotes )
T8044 366-368 , denotes ,
T8045 368-371 CC denotes and
T8046 372-374 CD denotes 50
T8047 375-377 NN denotes μl
T8049 378-395 NN denotes acetylthiocholine
T8048 396-404 NN denotes chloride
T8050 405-406 -LRB- denotes (
T8052 406-408 CD denotes 12
T8053 408-409 , denotes ,
T8054 409-410 CD denotes 5
T8051 411-413 NN denotes mM
T8055 414-416 IN denotes in
T8056 417-420 NN denotes H2O
T8057 420-421 -RRB- denotes )
T8058 422-426 VBD denotes were
T8004 427-432 VBN denotes added
T8059 432-433 . denotes .
T8060 433-575 sentence denotes The reaction took place at room temperature and was measured photometrically at a wavelength of 405 nm according to the Ellmann' method [25].
T8061 434-437 DT denotes The
T8062 438-446 NN denotes reaction
T8063 447-451 VBD denotes took
T8064 452-457 NN denotes place
T8065 458-460 IN denotes at
T8066 461-465 NN denotes room
T8067 466-477 NN denotes temperature
T8068 478-481 CC denotes and
T8069 482-485 VBD denotes was
T8070 486-494 VBN denotes measured
T8071 495-510 RB denotes photometrically
T8072 511-513 IN denotes at
T8073 514-515 DT denotes a
T8074 516-526 NN denotes wavelength
T8075 527-529 IN denotes of
T8076 530-533 CD denotes 405
T8077 534-536 NNS denotes nm
T8078 537-546 VBG denotes according
T8079 547-549 IN denotes to
T8080 550-553 DT denotes the
T8082 554-561 NNP denotes Ellmann
T8083 561-562 POS denotes '
T8081 563-569 NN denotes method
T8084 570-571 -LRB- denotes [
T8085 571-573 CD denotes 25
T8086 573-574 -RRB- denotes ]
T8087 574-575 . denotes .
R4913 T7951 T7952 compound Acetylcholine,esterase
R4914 T7952 T7953 compound esterase,assay
R4915 T7955 T7956 det A,sample
R4916 T7956 T7959 nsubjpass sample,mixed
R4917 T7957 T7958 nummod 30,μl
R4918 T7958 T7956 compound μl,sample
R4919 T7960 T7956 prep of,sample
R4920 T7961 T7962 amod red,cell
R4921 T7962 T7964 compound cell,vesicles
R4922 T7963 T7962 compound blood,cell
R4923 T7964 T7960 pobj vesicles,of
R4924 T7965 T7959 auxpass was,mixed
R4925 T7966 T7959 prep with,mixed
R4926 T7967 T7968 det an,volume
R4927 T7968 T7966 pobj volume,with
R4928 T7969 T7968 amod equal,volume
R4929 T7970 T7968 prep of,volume
R4930 T7971 T7970 pobj buffer,of
R4931 T7972 T7973 punct (,X
R4932 T7973 T7971 parataxis X,buffer
R4933 T7974 T7975 nummod 20,mM
R4934 T7975 T7976 compound mM,Hepes
R4935 T7976 T7973 dep Hepes,X
R4936 T7977 T7976 punct ", ",Hepes
R4937 T7978 T7976 npadvmod pH,Hepes
R4938 T7979 T7978 nummod 7.6,pH
R4939 T7980 T7973 punct ", ",X
R4940 T7981 T7982 nummod 150,mM
R4941 T7982 T7983 compound mM,NaCl
R4942 T7983 T7973 dep NaCl,X
R4943 T7984 T7973 punct ", ",X
R4944 T7985 T7986 nummod 0.5,%
R4945 T7986 T7973 compound %,X
R4946 T7987 T7973 compound Triton,X
R4947 T7988 T7973 punct -,X
R4948 T7989 T7973 nummod 100,X
R4949 T7990 T7973 punct ),X
R4950 T7991 T7959 punct ", ",mixed
R4951 T7992 T7959 conj vortexed,mixed
R4952 T7993 T7992 cc and,vortexed
R4953 T7994 T7992 conj incubated,vortexed
R4954 T7995 T7994 prep for,incubated
R4955 T7996 T7997 nummod 5,minutes
R4956 T7997 T7995 pobj minutes,for
R4957 T7998 T7994 prep at,incubated
R4958 T7999 T8000 nummod 37,°C
R4959 T8000 T7998 pobj °C,at
R4960 T8001 T7959 punct .,mixed
R4961 T8003 T8004 advmod Subsequently,added
R4962 T8005 T8006 nummod 640,μl
R4963 T8006 T8007 compound μl,buffer
R4964 T8007 T8004 nsubjpass buffer,added
R4965 T8008 T8009 compound sodium,phosphate
R4966 T8009 T8007 compound phosphate,buffer
R4967 T8010 T8011 punct (,Na2PO4
R4968 T8011 T8007 parataxis Na2PO4,buffer
R4969 T8012 T8013 nummod 100,mM
R4970 T8013 T8011 compound mM,Na2PO4
R4971 T8014 T8011 punct ", ",Na2PO4
R4972 T8015 T8011 npadvmod pH,Na2PO4
R4973 T8016 T8015 nummod 7.6,pH
R4974 T8017 T8011 punct ),Na2PO4
R4975 T8018 T8007 punct ", ",buffer
R4976 T8019 T8020 nummod 50,μl
R4977 T8020 T8021 compound μl,DTNB
R4978 T8021 T8007 conj DTNB,buffer
R4979 T8022 T8023 punct (,mM
R4980 T8023 T8021 parataxis mM,DTNB
R4981 T8024 T8025 nummod 5,dithiobis
R4982 T8025 T8023 dep dithiobis,mM
R4983 T8026 T8025 punct ",",dithiobis
R4984 T8027 T8025 nummod 5,dithiobis
R4985 T8028 T8027 punct ',5
R4986 T8029 T8025 punct -,dithiobis
R4987 T8030 T8023 punct -,mM
R4988 T8031 T8032 punct (,acid
R4989 T8032 T8023 parataxis acid,mM
R4990 T8033 T8032 nummod 2,acid
R4991 T8034 T8032 punct -,acid
R4992 T8035 T8032 amod nitrobenzoic,acid
R4993 T8036 T8032 punct ),acid
R4994 T8037 T8023 punct ", ",mM
R4995 T8038 T8023 nummod 10,mM
R4996 T8039 T8023 prep in,mM
R4997 T8040 T8041 compound sodium,phosphate
R4998 T8041 T8042 compound phosphate,buffer
R4999 T8042 T8039 pobj buffer,in
R5000 T8043 T8023 punct ),mM
R5001 T8044 T8021 punct ", ",DTNB
R5002 T8045 T8021 cc and,DTNB
R5003 T8046 T8047 nummod 50,μl
R5004 T8047 T8048 compound μl,chloride
R5005 T8048 T8021 conj chloride,DTNB
R5006 T8049 T8048 compound acetylthiocholine,chloride
R5007 T8050 T8051 punct (,mM
R5008 T8051 T8048 parataxis mM,chloride
R5009 T8052 T8051 nummod 12,mM
R5010 T8053 T8051 punct ",",mM
R5011 T8054 T8051 nummod 5,mM
R5012 T8055 T8051 prep in,mM
R5013 T8056 T8055 pobj H2O,in
R5014 T8057 T8051 punct ),mM
R5015 T8058 T8004 auxpass were,added
R5016 T8059 T8004 punct .,added
R5017 T8061 T8062 det The,reaction
R5018 T8062 T8063 nsubj reaction,took
R5019 T8064 T8063 dobj place,took
R5020 T8065 T8063 prep at,took
R5021 T8066 T8067 compound room,temperature
R5022 T8067 T8065 pobj temperature,at
R5023 T8068 T8063 cc and,took
R5024 T8069 T8070 auxpass was,measured
R5025 T8070 T8063 conj measured,took
R5026 T8071 T8070 advmod photometrically,measured
R5027 T8072 T8070 prep at,measured
R5028 T8073 T8074 det a,wavelength
R5029 T8074 T8072 pobj wavelength,at
R5030 T8075 T8074 prep of,wavelength
R5031 T8076 T8077 nummod 405,nm
R5032 T8077 T8075 pobj nm,of
R5033 T8078 T8070 prep according,measured
R5034 T8079 T8078 prep to,according
R5035 T8080 T8081 det the,method
R5036 T8081 T8079 pobj method,to
R5037 T8082 T8081 nmod Ellmann,method
R5038 T8083 T8081 case ',method
R5039 T8084 T8085 punct [,25
R5040 T8085 T8070 parataxis 25,measured
R5041 T8086 T8085 punct ],25
R5042 T8087 T8063 punct .,took