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PMC:194730 / 28698-29903 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T7273 0-4 NN denotes Self
T7274 5-12 VBG denotes forming
T7276 13-22 NN denotes iodixanol
T7277 23-30 NN denotes density
T7275 31-40 NNS denotes gradients
T7278 41-43 IN denotes of
T7279 44-49 JJ denotes human
T7281 50-53 JJ denotes red
T7282 54-59 NN denotes blood
T7280 60-65 NNS denotes cells
T7283 65-141 sentence denotes The subcellular distribution of annexin A7 was addressed according to [24].
T7284 66-69 DT denotes The
T7286 70-81 JJ denotes subcellular
T7285 82-94 NN denotes distribution
T7288 95-97 IN denotes of
T7289 98-105 NN denotes annexin
T7290 106-108 NN denotes A7
T7291 109-112 VBD denotes was
T7287 113-122 VBN denotes addressed
T7292 123-132 VBG denotes according
T7293 133-135 IN denotes to
T7294 136-137 -LRB- denotes [
T7295 137-139 CD denotes 24
T7296 139-140 -RRB- denotes ]
T7297 140-141 . denotes .
T7298 141-447 sentence denotes Red blood cells were centrifuged and washed in isotonic buffered salt solution, collected in ice cold lysis buffer (20 mM Hepes, pH 7.4, 200 μM CaCl2, 0.25 M sucrose, and proteinase inhibitors (Roche)), homogenized by a loosely fitting dounce homogenizer and additional passages through a 22 gauge needle.
T7299 142-145 JJ denotes Red
T7301 146-151 NN denotes blood
T7300 152-157 NNS denotes cells
T7303 158-162 VBD denotes were
T7302 163-174 VBN denotes centrifuged
T7304 175-178 CC denotes and
T7305 179-185 VBN denotes washed
T7306 186-188 IN denotes in
T7307 189-197 JJ denotes isotonic
T7309 198-206 VBN denotes buffered
T7310 207-211 NN denotes salt
T7308 212-220 NN denotes solution
T7311 220-222 , denotes ,
T7312 222-231 VBN denotes collected
T7313 232-234 IN denotes in
T7314 235-238 NN denotes ice
T7315 239-243 JJ denotes cold
T7317 244-249 NN denotes lysis
T7316 250-256 NN denotes buffer
T7318 257-258 -LRB- denotes (
T7320 258-260 CD denotes 20
T7321 261-263 NN denotes mM
T7319 264-269 NN denotes Hepes
T7322 269-271 , denotes ,
T7323 271-273 NN denotes pH
T7324 274-277 CD denotes 7.4
T7325 277-279 , denotes ,
T7326 279-282 CD denotes 200
T7327 283-285 NN denotes μM
T7328 286-291 NN denotes CaCl2
T7329 291-293 , denotes ,
T7330 293-297 CD denotes 0.25
T7331 298-299 NN denotes M
T7332 300-307 NN denotes sucrose
T7333 307-309 , denotes ,
T7334 309-312 CC denotes and
T7335 313-323 NN denotes proteinase
T7336 324-334 NNS denotes inhibitors
T7337 335-336 -LRB- denotes (
T7338 336-341 NNP denotes Roche
T7339 341-342 -RRB- denotes )
T7340 342-343 -RRB- denotes )
T7341 343-345 , denotes ,
T7342 345-356 VBN denotes homogenized
T7343 357-359 IN denotes by
T7344 360-361 DT denotes a
T7346 362-369 RB denotes loosely
T7347 370-377 JJ denotes fitting
T7348 378-384 NN denotes dounce
T7345 385-396 NN denotes homogenizer
T7349 397-400 CC denotes and
T7350 401-411 JJ denotes additional
T7351 412-420 NNS denotes passages
T7352 421-428 IN denotes through
T7353 429-430 DT denotes a
T7355 431-433 CD denotes 22
T7356 434-439 NN denotes gauge
T7354 440-446 NN denotes needle
T7357 446-447 . denotes .
T7358 447-607 sentence denotes A 50 % iodixanol solution was established by mixing Optiprep solution (60 % iodixanol; Sigma) with buffer (120 mM Hepes, pH 7.4, 1.2 mM CaCl2, 0.25 M sucrose).
T7359 448-449 DT denotes A
T7361 450-452 CD denotes 50
T7362 453-454 NN denotes %
T7363 455-464 NN denotes iodixanol
T7360 465-473 NN denotes solution
T7365 474-477 VBD denotes was
T7364 478-489 VBN denotes established
T7366 490-492 IN denotes by
T7367 493-499 VBG denotes mixing
T7368 500-508 NN denotes Optiprep
T7369 509-517 NN denotes solution
T7370 518-519 -LRB- denotes (
T7372 519-521 CD denotes 60
T7373 522-523 NN denotes %
T7374 524-533 NN denotes iodixanol
T7375 533-534 : denotes ;
T7371 535-540 NN denotes Sigma
T7376 540-541 -RRB- denotes )
T7377 542-546 IN denotes with
T7378 547-553 NN denotes buffer
T7379 554-555 -LRB- denotes (
T7381 555-558 CD denotes 120
T7382 559-561 NN denotes mM
T7383 562-567 NN denotes Hepes
T7384 567-569 , denotes ,
T7385 569-571 NN denotes pH
T7386 572-575 CD denotes 7.4
T7387 575-577 , denotes ,
T7388 577-580 CD denotes 1.2
T7389 581-583 NN denotes mM
T7390 584-589 NN denotes CaCl2
T7391 589-591 , denotes ,
T7392 591-595 CD denotes 0.25
T7393 596-597 NN denotes M
T7380 598-605 NN denotes sucrose
T7394 605-606 -RRB- denotes )
T7395 606-607 . denotes .
T7396 607-774 sentence denotes Seven parts of this 50 % iodixanol solution were mixed with thirteen parts of the red blood cell solution, generating a gradient solution containing 17.5 % iodixanol.
T7397 608-613 CD denotes Seven
T7398 614-619 NNS denotes parts
T7400 620-622 IN denotes of
T7401 623-627 DT denotes this
T7403 628-630 CD denotes 50
T7404 631-632 NN denotes %
T7405 633-642 NN denotes iodixanol
T7402 643-651 NN denotes solution
T7406 652-656 VBD denotes were
T7399 657-662 VBN denotes mixed
T7407 663-667 IN denotes with
T7408 668-676 CD denotes thirteen
T7409 677-682 NNS denotes parts
T7410 683-685 IN denotes of
T7411 686-689 DT denotes the
T7413 690-693 JJ denotes red
T7415 694-699 NN denotes blood
T7414 700-704 NN denotes cell
T7412 705-713 NN denotes solution
T7416 713-715 , denotes ,
T7417 715-725 VBG denotes generating
T7418 726-727 DT denotes a
T7420 728-736 NN denotes gradient
T7419 737-745 NN denotes solution
T7421 746-756 VBG denotes containing
T7422 757-761 CD denotes 17.5
T7423 762-763 NN denotes %
T7424 764-773 NN denotes iodixanol
T7425 773-774 . denotes .
T7426 774-1041 sentence denotes The mixture was filled into a centrifuge tube underlayed by cushions of 30 % and 35 % iodixanol and centrifuged for 3 hours at 270,000 × g in a SW41 Ti swing out rotor (Beckman) to achieve a nearly linear density gradient (approximately 1.06 g/ml to 1.20 g/ml) [24].
T7427 775-778 DT denotes The
T7428 779-786 NN denotes mixture
T7430 787-790 VBD denotes was
T7429 791-797 VBN denotes filled
T7431 798-802 IN denotes into
T7432 803-804 DT denotes a
T7434 805-815 JJ denotes centrifuge
T7433 816-820 NN denotes tube
T7435 821-831 VBN denotes underlayed
T7436 832-834 IN denotes by
T7437 835-843 NNS denotes cushions
T7438 844-846 IN denotes of
T7439 847-849 CD denotes 30
T7440 850-851 NN denotes %
T7442 852-855 CC denotes and
T7443 856-858 CD denotes 35
T7444 859-860 NN denotes %
T7441 861-870 NN denotes iodixanol
T7445 871-874 CC denotes and
T7446 875-886 VBN denotes centrifuged
T7447 887-890 IN denotes for
T7448 891-892 CD denotes 3
T7449 893-898 NNS denotes hours
T7450 899-901 IN denotes at
T7451 902-909 CD denotes 270,000
T7453 910-911 SYM denotes ×
T7452 912-913 NN denotes g
T7454 914-916 IN denotes in
T7455 917-918 DT denotes a
T7457 919-923 NN denotes SW41
T7458 924-926 NNP denotes Ti
T7459 927-932 NN denotes swing
T7460 933-936 NN denotes out
T7456 937-942 NN denotes rotor
T7461 943-944 -LRB- denotes (
T7462 944-951 NN denotes Beckman
T7463 951-952 -RRB- denotes )
T7464 953-955 TO denotes to
T7465 956-963 VB denotes achieve
T7466 964-965 DT denotes a
T7468 966-972 RB denotes nearly
T7469 973-979 JJ denotes linear
T7470 980-987 NN denotes density
T7467 988-996 NN denotes gradient
T7471 997-998 -LRB- denotes (
T7473 998-1011 RB denotes approximately
T7474 1012-1016 CD denotes 1.06
T7472 1017-1018 NNS denotes g
T7475 1018-1019 SYM denotes /
T7476 1019-1021 NN denotes ml
T7477 1022-1024 IN denotes to
T7478 1025-1029 CD denotes 1.20
T7479 1030-1031 NNS denotes g
T7480 1031-1032 SYM denotes /
T7481 1032-1034 NN denotes ml
T7482 1034-1035 -RRB- denotes )
T7483 1036-1037 -LRB- denotes [
T7484 1037-1039 CD denotes 24
T7485 1039-1040 -RRB- denotes ]
T7486 1040-1041 . denotes .
T7487 1041-1205 sentence denotes Fractions of 0.6 ml were collected from the top of the gradient by a 1 ml syringe without using a needle and immediately frozen in liquid nitrogen for further use.
T7488 1042-1051 NNS denotes Fractions
T7490 1052-1054 IN denotes of
T7491 1055-1058 CD denotes 0.6
T7492 1059-1061 NN denotes ml
T7493 1062-1066 VBD denotes were
T7489 1067-1076 VBN denotes collected
T7494 1077-1081 IN denotes from
T7495 1082-1085 DT denotes the
T7496 1086-1089 NN denotes top
T7497 1090-1092 IN denotes of
T7498 1093-1096 DT denotes the
T7499 1097-1105 NN denotes gradient
T7500 1106-1108 IN denotes by
T7501 1109-1110 DT denotes a
T7503 1111-1112 CD denotes 1
T7504 1113-1115 NN denotes ml
T7502 1116-1123 NN denotes syringe
T7505 1124-1131 IN denotes without
T7506 1132-1137 VBG denotes using
T7507 1138-1139 DT denotes a
T7508 1140-1146 NN denotes needle
T7509 1147-1150 CC denotes and
T7510 1151-1162 RB denotes immediately
T7511 1163-1169 VBN denotes frozen
T7512 1170-1172 IN denotes in
T7513 1173-1179 JJ denotes liquid
T7514 1180-1188 NN denotes nitrogen
T7515 1189-1192 IN denotes for
T7516 1193-1200 JJ denotes further
T7517 1201-1204 NN denotes use
T7518 1204-1205 . denotes .
R4431 T7273 T7274 npadvmod Self,forming
R4432 T7274 T7275 amod forming,gradients
R4433 T7276 T7275 compound iodixanol,gradients
R4434 T7277 T7275 compound density,gradients
R4435 T7278 T7275 prep of,gradients
R4436 T7279 T7280 amod human,cells
R4437 T7280 T7278 pobj cells,of
R4438 T7281 T7280 amod red,cells
R4439 T7282 T7280 compound blood,cells
R4440 T7284 T7285 det The,distribution
R4441 T7285 T7287 nsubjpass distribution,addressed
R4442 T7286 T7285 amod subcellular,distribution
R4443 T7288 T7285 prep of,distribution
R4444 T7289 T7290 compound annexin,A7
R4445 T7290 T7288 pobj A7,of
R4446 T7291 T7287 auxpass was,addressed
R4447 T7292 T7287 prep according,addressed
R4448 T7293 T7292 prep to,according
R4449 T7294 T7293 punct [,to
R4450 T7295 T7293 pobj 24,to
R4451 T7296 T7287 punct ],addressed
R4452 T7297 T7287 punct .,addressed
R4453 T7299 T7300 amod Red,cells
R4454 T7300 T7302 nsubjpass cells,centrifuged
R4455 T7301 T7300 compound blood,cells
R4456 T7303 T7302 auxpass were,centrifuged
R4457 T7304 T7302 cc and,centrifuged
R4458 T7305 T7302 conj washed,centrifuged
R4459 T7306 T7305 prep in,washed
R4460 T7307 T7308 amod isotonic,solution
R4461 T7308 T7306 pobj solution,in
R4462 T7309 T7310 amod buffered,salt
R4463 T7310 T7308 compound salt,solution
R4464 T7311 T7305 punct ", ",washed
R4465 T7312 T7305 conj collected,washed
R4466 T7313 T7312 prep in,collected
R4467 T7314 T7315 npadvmod ice,cold
R4468 T7315 T7316 amod cold,buffer
R4469 T7316 T7313 pobj buffer,in
R4470 T7317 T7316 compound lysis,buffer
R4471 T7318 T7319 punct (,Hepes
R4472 T7319 T7316 parataxis Hepes,buffer
R4473 T7320 T7321 nummod 20,mM
R4474 T7321 T7319 compound mM,Hepes
R4475 T7322 T7319 punct ", ",Hepes
R4476 T7323 T7319 npadvmod pH,Hepes
R4477 T7324 T7323 nummod 7.4,pH
R4478 T7325 T7319 punct ", ",Hepes
R4479 T7326 T7327 nummod 200,μM
R4480 T7327 T7328 compound μM,CaCl2
R4481 T7328 T7319 conj CaCl2,Hepes
R4482 T7329 T7328 punct ", ",CaCl2
R4483 T7330 T7331 nummod 0.25,M
R4484 T7331 T7332 compound M,sucrose
R4485 T7332 T7328 conj sucrose,CaCl2
R4486 T7333 T7332 punct ", ",sucrose
R4487 T7334 T7332 cc and,sucrose
R4488 T7335 T7336 compound proteinase,inhibitors
R4489 T7336 T7332 conj inhibitors,sucrose
R4490 T7337 T7338 punct (,Roche
R4491 T7338 T7336 parataxis Roche,inhibitors
R4492 T7339 T7338 punct ),Roche
R4493 T7340 T7336 punct ),inhibitors
R4494 T7341 T7312 punct ", ",collected
R4495 T7342 T7312 conj homogenized,collected
R4496 T7343 T7342 prep by,homogenized
R4497 T7344 T7345 det a,homogenizer
R4498 T7345 T7343 pobj homogenizer,by
R4499 T7346 T7347 advmod loosely,fitting
R4500 T7347 T7345 amod fitting,homogenizer
R4501 T7348 T7345 compound dounce,homogenizer
R4502 T7349 T7345 cc and,homogenizer
R4503 T7350 T7351 amod additional,passages
R4504 T7351 T7345 conj passages,homogenizer
R4505 T7352 T7351 prep through,passages
R4506 T7353 T7354 det a,needle
R4507 T7354 T7352 pobj needle,through
R4508 T7355 T7356 nummod 22,gauge
R4509 T7356 T7354 compound gauge,needle
R4510 T7357 T7302 punct .,centrifuged
R4511 T7359 T7360 det A,solution
R4512 T7360 T7364 nsubjpass solution,established
R4513 T7361 T7362 nummod 50,%
R4514 T7362 T7360 compound %,solution
R4515 T7363 T7360 compound iodixanol,solution
R4516 T7365 T7364 auxpass was,established
R4517 T7366 T7364 prep by,established
R4518 T7367 T7366 pcomp mixing,by
R4519 T7368 T7369 compound Optiprep,solution
R4520 T7369 T7367 dobj solution,mixing
R4521 T7370 T7371 punct (,Sigma
R4522 T7371 T7369 parataxis Sigma,solution
R4523 T7372 T7373 nummod 60,%
R4524 T7373 T7374 compound %,iodixanol
R4525 T7374 T7371 dep iodixanol,Sigma
R4526 T7375 T7371 punct ;,Sigma
R4527 T7376 T7371 punct ),Sigma
R4528 T7377 T7367 prep with,mixing
R4529 T7378 T7377 pobj buffer,with
R4530 T7379 T7380 punct (,sucrose
R4531 T7380 T7378 parataxis sucrose,buffer
R4532 T7381 T7382 nummod 120,mM
R4533 T7382 T7383 compound mM,Hepes
R4534 T7383 T7380 dep Hepes,sucrose
R4535 T7384 T7383 punct ", ",Hepes
R4536 T7385 T7383 npadvmod pH,Hepes
R4537 T7386 T7385 nummod 7.4,pH
R4538 T7387 T7380 punct ", ",sucrose
R4539 T7388 T7389 nummod 1.2,mM
R4540 T7389 T7390 compound mM,CaCl2
R4541 T7390 T7380 dep CaCl2,sucrose
R4542 T7391 T7380 punct ", ",sucrose
R4543 T7392 T7393 nummod 0.25,M
R4544 T7393 T7380 compound M,sucrose
R4545 T7394 T7380 punct ),sucrose
R4546 T7395 T7364 punct .,established
R4547 T7397 T7398 nummod Seven,parts
R4548 T7398 T7399 nsubjpass parts,mixed
R4549 T7400 T7398 prep of,parts
R4550 T7401 T7402 det this,solution
R4551 T7402 T7400 pobj solution,of
R4552 T7403 T7404 nummod 50,%
R4553 T7404 T7402 compound %,solution
R4554 T7405 T7402 compound iodixanol,solution
R4555 T7406 T7399 auxpass were,mixed
R4556 T7407 T7399 prep with,mixed
R4557 T7408 T7409 nummod thirteen,parts
R4558 T7409 T7407 pobj parts,with
R4559 T7410 T7409 prep of,parts
R4560 T7411 T7412 det the,solution
R4561 T7412 T7410 pobj solution,of
R4562 T7413 T7414 amod red,cell
R4563 T7414 T7412 compound cell,solution
R4564 T7415 T7414 compound blood,cell
R4565 T7416 T7399 punct ", ",mixed
R4566 T7417 T7399 advcl generating,mixed
R4567 T7418 T7419 det a,solution
R4568 T7419 T7417 dobj solution,generating
R4569 T7420 T7419 compound gradient,solution
R4570 T7421 T7419 acl containing,solution
R4571 T7422 T7423 nummod 17.5,%
R4572 T7423 T7424 compound %,iodixanol
R4573 T7424 T7421 dobj iodixanol,containing
R4574 T7425 T7399 punct .,mixed
R4575 T7427 T7428 det The,mixture
R4576 T7428 T7429 nsubj mixture,filled
R4577 T7430 T7429 aux was,filled
R4578 T7431 T7429 prep into,filled
R4579 T7432 T7433 det a,tube
R4580 T7433 T7431 pobj tube,into
R4581 T7434 T7433 amod centrifuge,tube
R4582 T7435 T7433 amod underlayed,tube
R4583 T7436 T7435 prep by,underlayed
R4584 T7437 T7436 pobj cushions,by
R4585 T7438 T7437 prep of,cushions
R4586 T7439 T7440 nummod 30,%
R4587 T7440 T7441 nmod %,iodixanol
R4588 T7441 T7438 pobj iodixanol,of
R4589 T7442 T7440 cc and,%
R4590 T7443 T7444 nummod 35,%
R4591 T7444 T7440 conj %,%
R4592 T7445 T7429 cc and,filled
R4593 T7446 T7429 conj centrifuged,filled
R4594 T7447 T7446 prep for,centrifuged
R4595 T7448 T7449 nummod 3,hours
R4596 T7449 T7447 pobj hours,for
R4597 T7450 T7446 prep at,centrifuged
R4598 T7451 T7452 nummod "270,000",g
R4599 T7452 T7450 pobj g,at
R4600 T7453 T7452 punct ×,g
R4601 T7454 T7446 prep in,centrifuged
R4602 T7455 T7456 det a,rotor
R4603 T7456 T7454 pobj rotor,in
R4604 T7457 T7458 compound SW41,Ti
R4605 T7458 T7456 compound Ti,rotor
R4606 T7459 T7460 compound swing,out
R4607 T7460 T7456 compound out,rotor
R4608 T7461 T7462 punct (,Beckman
R4609 T7462 T7456 parataxis Beckman,rotor
R4610 T7463 T7462 punct ),Beckman
R4611 T7464 T7465 aux to,achieve
R4612 T7465 T7429 advcl achieve,filled
R4613 T7466 T7467 det a,gradient
R4614 T7467 T7465 dobj gradient,achieve
R4615 T7468 T7469 advmod nearly,linear
R4616 T7469 T7467 amod linear,gradient
R4617 T7470 T7467 compound density,gradient
R4618 T7471 T7472 punct (,g
R4619 T7472 T7467 parataxis g,gradient
R4620 T7473 T7472 advmod approximately,g
R4621 T7474 T7472 nummod 1.06,g
R4622 T7475 T7476 punct /,ml
R4623 T7476 T7472 prep ml,g
R4624 T7477 T7472 prep to,g
R4625 T7478 T7479 nummod 1.20,g
R4626 T7479 T7477 pobj g,to
R4627 T7480 T7481 punct /,ml
R4628 T7481 T7479 prep ml,g
R4629 T7482 T7472 punct ),g
R4630 T7483 T7484 punct [,24
R4631 T7484 T7429 parataxis 24,filled
R4632 T7485 T7484 punct ],24
R4633 T7486 T7429 punct .,filled
R4634 T7488 T7489 nsubjpass Fractions,collected
R4635 T7490 T7488 prep of,Fractions
R4636 T7491 T7492 nummod 0.6,ml
R4637 T7492 T7490 pobj ml,of
R4638 T7493 T7489 auxpass were,collected
R4639 T7494 T7489 prep from,collected
R4640 T7495 T7496 det the,top
R4641 T7496 T7494 pobj top,from
R4642 T7497 T7496 prep of,top
R4643 T7498 T7499 det the,gradient
R4644 T7499 T7497 pobj gradient,of
R4645 T7500 T7489 prep by,collected
R4646 T7501 T7502 det a,syringe
R4647 T7502 T7500 pobj syringe,by
R4648 T7503 T7504 nummod 1,ml
R4649 T7504 T7502 compound ml,syringe
R4650 T7505 T7489 prep without,collected
R4651 T7506 T7505 pcomp using,without
R4652 T7507 T7508 det a,needle
R4653 T7508 T7506 dobj needle,using
R4654 T7509 T7489 cc and,collected
R4655 T7510 T7511 advmod immediately,frozen
R4656 T7511 T7489 conj frozen,collected
R4657 T7512 T7511 prep in,frozen
R4658 T7513 T7514 amod liquid,nitrogen
R4659 T7514 T7512 pobj nitrogen,in
R4660 T7515 T7511 prep for,frozen
R4661 T7516 T7517 amod further,use
R4662 T7517 T7515 pobj use,for
R4663 T7518 T7489 punct .,collected