PMC:194730 JSON TXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T205	0-8	NN	denotes	Function
T207	0-134	sentence	denotes	Function, expression and localization of annexin A7 in platelets and red blood cells: Insights derived from an annexin A7 mutant mouse
T208	8-10	,	denotes	,
T209	10-20	NN	denotes	expression
T210	21-24	CC	denotes	and
T211	25-37	NN	denotes	localization
T212	38-40	IN	denotes	of
T213	41-48	NN	denotes	annexin
T214	49-51	NN	denotes	A7
T215	52-54	IN	denotes	in
T216	55-64	NNS	denotes	platelets
T217	65-68	CC	denotes	and
T218	69-72	JJ	denotes	red
T220	73-78	NN	denotes	blood
T219	79-84	NNS	denotes	cells
T221	84-86	:	denotes	:
T206	86-94	NNS	denotes	Insights
T222	95-102	VBN	denotes	derived
T223	103-107	IN	denotes	from
T224	108-110	DT	denotes	an
T226	111-118	NN	denotes	annexin
T227	119-121	NN	denotes	A7
T228	122-128	JJ	denotes	mutant
T225	129-134	NN	denotes	mouse
T229	134-135	sentence	denotes
T230	145-155	sentence	denotes	Background
T231	145-155	NN	denotes	Background
T232	155-310	sentence	denotes	Annexin A7 is a Ca2+- and phospholipid-binding protein expressed as a 47 and 51 kDa isoform, which is thought to be involved in membrane fusion processes.
T233	156-163	NN	denotes	Annexin
T234	164-166	NN	denotes	A7
T235	167-169	VBZ	denotes	is
T236	170-171	DT	denotes	a
T238	172-176	NN	denotes	Ca2+
T240	176-177	HYPH	denotes	-
T241	178-181	CC	denotes	and
T242	182-194	NN	denotes	phospholipid
T243	194-195	HYPH	denotes	-
T239	195-202	VBG	denotes	binding
T237	203-210	NN	denotes	protein
T244	211-220	VBN	denotes	expressed
T245	221-223	IN	denotes	as
T246	224-225	DT	denotes	a
T248	226-228	CD	denotes	47
T250	229-232	CC	denotes	and
T251	233-235	CD	denotes	51
T249	236-239	NN	denotes	kDa
T247	240-247	NN	denotes	isoform
T252	247-249	,	denotes	,
T253	249-254	WDT	denotes	which
T255	255-257	VBZ	denotes	is
T254	258-265	VBN	denotes	thought
T256	266-268	TO	denotes	to
T258	269-271	VB	denotes	be
T257	272-280	VBN	denotes	involved
T259	281-283	IN	denotes	in
T260	284-292	NN	denotes	membrane
T261	293-299	NN	denotes	fusion
T262	300-309	NNS	denotes	processes
T263	309-310	.	denotes	.
T264	310-590	sentence	denotes	Recently the 47 kDa isoform has been identified in erythrocytes where it was proposed to be a key component in the process of the Ca2+-dependent vesicle release, a process with which red blood cells might protect themselves against an attack by for example complement components.
T265	311-319	RB	denotes	Recently
T267	320-323	DT	denotes	the
T269	324-326	CD	denotes	47
T270	327-330	NN	denotes	kDa
T268	331-338	NN	denotes	isoform
T271	339-342	VBZ	denotes	has
T272	343-347	VBN	denotes	been
T266	348-358	VBN	denotes	identified
T273	359-361	IN	denotes	in
T274	362-374	NNS	denotes	erythrocytes
T275	375-380	WRB	denotes	where
T277	381-383	PRP	denotes	it
T278	384-387	VBD	denotes	was
T276	388-396	VBN	denotes	proposed
T279	397-399	TO	denotes	to
T280	400-402	VB	denotes	be
T281	403-404	DT	denotes	a
T283	405-408	JJ	denotes	key
T282	409-418	NN	denotes	component
T284	419-421	IN	denotes	in
T285	422-425	DT	denotes	the
T286	426-433	NN	denotes	process
T287	434-436	IN	denotes	of
T288	437-440	DT	denotes	the
T290	441-445	NN	denotes	Ca2+
T292	445-446	HYPH	denotes	-
T291	446-455	JJ	denotes	dependent
T293	456-463	NN	denotes	vesicle
T289	464-471	NN	denotes	release
T294	471-473	,	denotes	,
T295	473-474	DT	denotes	a
T296	475-482	NN	denotes	process
T297	483-487	IN	denotes	with
T299	488-493	WDT	denotes	which
T300	494-497	JJ	denotes	red
T302	498-503	NN	denotes	blood
T301	504-509	NNS	denotes	cells
T303	510-515	MD	denotes	might
T298	516-523	VB	denotes	protect
T304	524-534	PRP	denotes	themselves
T305	535-542	IN	denotes	against
T306	543-545	DT	denotes	an
T307	546-552	NN	denotes	attack
T308	553-555	IN	denotes	by
T309	556-559	IN	denotes	for
T311	560-567	NN	denotes	example
T312	568-578	NN	denotes	complement
T310	579-589	NNS	denotes	components
T313	589-590	.	denotes	.
T314	590-599	sentence	denotes	Results
T315	592-599	NNS	denotes	Results
T316	599-691	sentence	denotes	The role of annexin A7 in red blood cells was addressed in erythrocytes from anxA7-/- mice.
T317	600-603	DT	denotes	The
T318	604-608	NN	denotes	role
T320	609-611	IN	denotes	of
T321	612-619	NN	denotes	annexin
T322	620-622	NN	denotes	A7
T323	623-625	IN	denotes	in
T324	626-629	JJ	denotes	red
T326	630-635	NN	denotes	blood
T325	636-641	NNS	denotes	cells
T327	642-645	VBD	denotes	was
T319	646-655	VBN	denotes	addressed
T328	656-658	IN	denotes	in
T329	659-671	NNS	denotes	erythrocytes
T330	672-676	IN	denotes	from
T331	677-682	JJ	denotes	anxA7
T333	682-683	SYM	denotes	-
T334	683-684	HYPH	denotes	/
T335	684-685	SYM	denotes	-
T332	686-690	NNS	denotes	mice
T336	690-691	.	denotes	.
T337	691-763	sentence	denotes	Interestingly, the Ca2+-mediated vesiculation process was not impaired.
T338	692-705	RB	denotes	Interestingly
T340	705-707	,	denotes	,
T341	707-710	DT	denotes	the
T343	711-715	NN	denotes	Ca2+
T345	715-716	HYPH	denotes	-
T344	716-724	VBN	denotes	mediated
T346	725-737	NN	denotes	vesiculation
T342	738-745	NN	denotes	process
T347	746-749	VBD	denotes	was
T348	750-753	RB	denotes	not
T339	754-762	VBN	denotes	impaired
T349	762-763	.	denotes	.
T350	763-874	sentence	denotes	Also, the membrane organization appeared not to be disturbed as assessed using gradient fractionation studies.
T351	764-768	RB	denotes	Also
T353	768-770	,	denotes	,
T354	770-773	DT	denotes	the
T356	774-782	NN	denotes	membrane
T355	783-795	NN	denotes	organization
T352	796-804	VBD	denotes	appeared
T357	805-808	RB	denotes	not
T359	809-811	TO	denotes	to
T360	812-814	VB	denotes	be
T358	815-824	VBN	denotes	disturbed
T361	825-827	IN	denotes	as
T362	828-836	VBN	denotes	assessed
T363	837-842	VBG	denotes	using
T364	843-851	NN	denotes	gradient
T365	852-865	NN	denotes	fractionation
T366	866-873	NNS	denotes	studies
T367	873-874	.	denotes	.
T368	874-984	sentence	denotes	Instead, lack of annexin A7 led to an altered cell shape and increased osmotic resistance of red blood cells.
T369	875-882	RB	denotes	Instead
T371	882-884	,	denotes	,
T372	884-888	NN	denotes	lack
T373	889-891	IN	denotes	of
T374	892-899	NN	denotes	annexin
T375	900-902	NN	denotes	A7
T370	903-906	VBD	denotes	led
T376	907-909	IN	denotes	to
T377	910-912	DT	denotes	an
T379	913-920	JJ	denotes	altered
T380	921-925	NN	denotes	cell
T378	926-931	NN	denotes	shape
T381	932-935	CC	denotes	and
T382	936-945	VBN	denotes	increased
T384	946-953	JJ	denotes	osmotic
T383	954-964	NN	denotes	resistance
T385	965-967	IN	denotes	of
T386	968-971	JJ	denotes	red
T388	972-977	NN	denotes	blood
T387	978-983	NNS	denotes	cells
T389	983-984	.	denotes	.
T390	984-1029	sentence	denotes	Annexin A7 was also identified in platelets.
T391	985-992	NN	denotes	Annexin
T392	993-995	NN	denotes	A7
T394	996-999	VBD	denotes	was
T395	1000-1004	RB	denotes	also
T393	1005-1015	VBN	denotes	identified
T396	1016-1018	IN	denotes	in
T397	1019-1028	NNS	denotes	platelets
T398	1028-1029	.	denotes	.
T399	1029-1166	sentence	denotes	In these cells its loss led to a slightly slower aggregation velocity which seems to be compensated by an increased number of platelets.
T400	1030-1032	IN	denotes	In
T402	1033-1038	DT	denotes	these
T403	1039-1044	NNS	denotes	cells
T404	1045-1048	PRP$	denotes	its
T405	1049-1053	NN	denotes	loss
T401	1054-1057	VBD	denotes	led
T406	1058-1060	IN	denotes	to
T407	1061-1062	DT	denotes	a
T409	1063-1071	RB	denotes	slightly
T410	1072-1078	JJR	denotes	slower
T411	1079-1090	NN	denotes	aggregation
T408	1091-1099	NN	denotes	velocity
T412	1100-1105	WDT	denotes	which
T413	1106-1111	VBZ	denotes	seems
T414	1112-1114	TO	denotes	to
T416	1115-1117	VB	denotes	be
T415	1118-1129	VBN	denotes	compensated
T417	1130-1132	IN	denotes	by
T418	1133-1135	DT	denotes	an
T420	1136-1145	VBN	denotes	increased
T419	1146-1152	NN	denotes	number
T421	1153-1155	IN	denotes	of
T422	1156-1165	NNS	denotes	platelets
T423	1165-1166	.	denotes	.
T424	1166-1288	sentence	denotes	The results appear to rule out an important role of annexin A7 in membrane fusion processes occurring in red blood cells.
T425	1167-1170	DT	denotes	The
T426	1171-1178	NNS	denotes	results
T427	1179-1185	VBP	denotes	appear
T428	1186-1188	TO	denotes	to
T429	1189-1193	VB	denotes	rule
T430	1194-1197	RP	denotes	out
T431	1198-1200	DT	denotes	an
T433	1201-1210	JJ	denotes	important
T432	1211-1215	NN	denotes	role
T434	1216-1218	IN	denotes	of
T435	1219-1226	NN	denotes	annexin
T436	1227-1229	NN	denotes	A7
T437	1230-1232	IN	denotes	in
T438	1233-1241	NN	denotes	membrane
T439	1242-1248	NN	denotes	fusion
T440	1249-1258	NNS	denotes	processes
T441	1259-1268	VBG	denotes	occurring
T442	1269-1271	IN	denotes	in
T443	1272-1275	JJ	denotes	red
T445	1276-1281	NN	denotes	blood
T444	1282-1287	NNS	denotes	cells
T446	1287-1288	.	denotes	.
T447	1288-1376	sentence	denotes	Instead the protein might be involved in the organization of the membrane cytoskeleton.
T448	1289-1296	RB	denotes	Instead
T450	1297-1300	DT	denotes	the
T451	1301-1308	NN	denotes	protein
T452	1309-1314	MD	denotes	might
T453	1315-1317	VB	denotes	be
T449	1318-1326	VBN	denotes	involved
T454	1327-1329	IN	denotes	in
T455	1330-1333	DT	denotes	the
T456	1334-1346	NN	denotes	organization
T457	1347-1349	IN	denotes	of
T458	1350-1353	DT	denotes	the
T460	1354-1362	NN	denotes	membrane
T459	1363-1375	NN	denotes	cytoskeleton
T461	1375-1376	.	denotes	.
T462	1376-1482	sentence	denotes	Red blood cells may represent an appropriate model to study the role of annexin A7 in cellular processes.
T463	1377-1380	JJ	denotes	Red
T465	1381-1386	NN	denotes	blood
T464	1387-1392	NNS	denotes	cells
T467	1393-1396	MD	denotes	may
T466	1397-1406	VB	denotes	represent
T468	1407-1409	DT	denotes	an
T470	1410-1421	JJ	denotes	appropriate
T469	1422-1427	NN	denotes	model
T471	1428-1430	TO	denotes	to
T472	1431-1436	VB	denotes	study
T473	1437-1440	DT	denotes	the
T474	1441-1445	NN	denotes	role
T475	1446-1448	IN	denotes	of
T476	1449-1456	NN	denotes	annexin
T477	1457-1459	NN	denotes	A7
T478	1460-1462	IN	denotes	in
T479	1463-1471	JJ	denotes	cellular
T480	1472-1481	NNS	denotes	processes
T481	1481-1482	.	denotes	.
T482	1482-1494	sentence	denotes	Conclusion
T483	1484-1494	NN	denotes	Conclusion
T484	1494-1627	sentence	denotes	We have demonstrated the presence of both annexin A7 isoforms in red blood cells and the presence of the small isoform in platelets.
T485	1495-1497	PRP	denotes	We
T487	1498-1502	VBP	denotes	have
T486	1503-1515	VBN	denotes	demonstrated
T488	1516-1519	DT	denotes	the
T489	1520-1528	NN	denotes	presence
T490	1529-1531	IN	denotes	of
T491	1532-1536	CC	denotes	both
T493	1537-1544	NN	denotes	annexin
T494	1545-1547	NN	denotes	A7
T492	1548-1556	NNS	denotes	isoforms
T495	1557-1559	IN	denotes	in
T496	1560-1563	JJ	denotes	red
T498	1564-1569	NN	denotes	blood
T497	1570-1575	NNS	denotes	cells
T499	1576-1579	CC	denotes	and
T500	1580-1583	DT	denotes	the
T501	1584-1592	NN	denotes	presence
T502	1593-1595	IN	denotes	of
T503	1596-1599	DT	denotes	the
T505	1600-1605	JJ	denotes	small
T504	1606-1613	NN	denotes	isoform
T506	1614-1616	IN	denotes	in
T507	1617-1626	NNS	denotes	platelets
T508	1626-1627	.	denotes	.
T509	1627-1697	sentence	denotes	In both cell types the loss of annexin A7 impairs cellular functions.
T510	1628-1630	IN	denotes	In
T512	1631-1635	DT	denotes	both
T514	1636-1640	NN	denotes	cell
T513	1641-1646	NNS	denotes	types
T515	1647-1650	DT	denotes	the
T516	1651-1655	NN	denotes	loss
T517	1656-1658	IN	denotes	of
T518	1659-1666	NN	denotes	annexin
T519	1667-1669	NN	denotes	A7
T511	1670-1677	VBZ	denotes	impairs
T520	1678-1686	JJ	denotes	cellular
T521	1687-1696	NNS	denotes	functions
T522	1696-1697	.	denotes	.
T523	1697-1830	sentence	denotes	The defects observed are however not compatible with a crucial role for annexin A7 in membrane fusion processes in these cell types.
T524	1698-1701	DT	denotes	The
T525	1702-1709	NNS	denotes	defects
T527	1710-1718	VBN	denotes	observed
T526	1719-1722	VBP	denotes	are
T528	1723-1730	RB	denotes	however
T529	1731-1734	RB	denotes	not
T530	1735-1745	JJ	denotes	compatible
T531	1746-1750	IN	denotes	with
T532	1751-1752	DT	denotes	a
T534	1753-1760	JJ	denotes	crucial
T533	1761-1765	NN	denotes	role
T535	1766-1769	IN	denotes	for
T536	1770-1777	NN	denotes	annexin
T537	1778-1780	NN	denotes	A7
T538	1781-1783	IN	denotes	in
T539	1784-1792	NN	denotes	membrane
T540	1793-1799	NN	denotes	fusion
T541	1800-1809	NNS	denotes	processes
T542	1810-1812	IN	denotes	in
T543	1813-1818	DT	denotes	these
T545	1819-1823	NN	denotes	cell
T544	1824-1829	NNS	denotes	types
T546	1829-1830	.	denotes	.
T1134	1843-1850	NN	denotes	Annexin
T1135	1851-1853	NN	denotes	A7
T1136	1854-1856	VBZ	denotes	is
T1137	1857-1858	DT	denotes	a
T1139	1859-1863	NN	denotes	Ca2+
T1141	1863-1864	HYPH	denotes	-
T1142	1865-1868	CC	denotes	and
T1143	1869-1881	NN	denotes	phospholipid
T1144	1881-1882	HYPH	denotes	-
T1140	1882-1889	VBG	denotes	binding
T1138	1890-1897	NN	denotes	protein
T1145	1897-1899	,	denotes	,
T1146	1899-1904	WDT	denotes	which
T1148	1905-1908	VBD	denotes	was
T1147	1909-1917	VBN	denotes	isolated
T1149	1918-1920	IN	denotes	as
T1150	1921-1924	DT	denotes	the
T1151	1925-1930	NN	denotes	agent
T1152	1931-1935	WDT	denotes	that
T1153	1936-1944	VBD	denotes	mediated
T1154	1945-1956	NN	denotes	aggregation
T1155	1957-1959	IN	denotes	of
T1156	1960-1970	NN	denotes	chromaffin
T1157	1971-1979	NNS	denotes	granules
T1158	1980-1983	CC	denotes	and
T1159	1984-1990	NN	denotes	fusion
T1160	1991-1993	IN	denotes	of
T1161	1994-2006	NN	denotes	phospholipid
T1162	2007-2016	NNS	denotes	membranes
T1163	2017-2019	IN	denotes	in
T1164	2020-2023	DT	denotes	the
T1165	2024-2032	NN	denotes	presence
T1166	2033-2035	IN	denotes	of
T1167	2036-2040	NN	denotes	Ca2+
T1168	2040-2041	.	denotes	.
T1169	2041-2154	sentence	denotes	This activity led to the proposal of annexin A7's involvement in the exocytotic secretion of catecholamines [1].
T1170	2042-2046	DT	denotes	This
T1171	2047-2055	NN	denotes	activity
T1172	2056-2059	VBD	denotes	led
T1173	2060-2062	IN	denotes	to
T1174	2063-2066	DT	denotes	the
T1175	2067-2075	NN	denotes	proposal
T1176	2076-2078	IN	denotes	of
T1177	2079-2086	NN	denotes	annexin
T1178	2087-2089	NN	denotes	A7
T1180	2089-2091	POS	denotes	's
T1179	2092-2103	NN	denotes	involvement
T1181	2104-2106	IN	denotes	in
T1182	2107-2110	DT	denotes	the
T1184	2111-2121	JJ	denotes	exocytotic
T1183	2122-2131	NN	denotes	secretion
T1185	2132-2134	IN	denotes	of
T1186	2135-2149	NNS	denotes	catecholamines
T1187	2150-2151	-LRB-	denotes	[
T1188	2151-2152	CD	denotes	1
T1189	2152-2153	-RRB-	denotes	]
T1190	2153-2154	.	denotes	.
T1191	2154-2310	sentence	denotes	The protein belongs to a family of evolutionarily conserved proteins of a bipartite structure with a variable N-terminal and a conserved C-terminal domain.
T1192	2155-2158	DT	denotes	The
T1193	2159-2166	NN	denotes	protein
T1194	2167-2174	VBZ	denotes	belongs
T1195	2175-2177	IN	denotes	to
T1196	2178-2179	DT	denotes	a
T1197	2180-2186	NN	denotes	family
T1198	2187-2189	IN	denotes	of
T1199	2190-2204	RB	denotes	evolutionarily
T1200	2205-2214	VBN	denotes	conserved
T1201	2215-2223	NN	denotes	proteins
T1202	2224-2226	IN	denotes	of
T1203	2227-2228	DT	denotes	a
T1205	2229-2238	JJ	denotes	bipartite
T1204	2239-2248	NN	denotes	structure
T1206	2249-2253	IN	denotes	with
T1207	2254-2255	DT	denotes	a
T1208	2256-2264	JJ	denotes	variable
T1209	2265-2266	NN	denotes	N
T1211	2266-2267	HYPH	denotes	-
T1210	2267-2275	JJ	denotes	terminal
T1212	2276-2279	CC	denotes	and
T1213	2280-2281	DT	denotes	a
T1214	2282-2291	VBN	denotes	conserved
T1215	2292-2293	NN	denotes	C
T1217	2293-2294	HYPH	denotes	-
T1216	2294-2302	JJ	denotes	terminal
T1218	2303-2309	NN	denotes	domain
T1219	2309-2310	.	denotes	.
T1220	2310-2455	sentence	denotes	The C-terminal domain is responsible for the Ca2+- and phospholipid-binding, the N-terminal domain appears to confer functional diversity [2-4].
T1221	2311-2314	DT	denotes	The
T1223	2315-2316	NN	denotes	C
T1225	2316-2317	HYPH	denotes	-
T1224	2317-2325	JJ	denotes	terminal
T1222	2326-2332	NN	denotes	domain
T1226	2333-2335	VBZ	denotes	is
T1228	2336-2347	JJ	denotes	responsible
T1229	2348-2351	IN	denotes	for
T1230	2352-2355	DT	denotes	the
T1231	2356-2360	NN	denotes	Ca2+
T1232	2360-2361	HYPH	denotes	-
T1233	2362-2365	CC	denotes	and
T1234	2366-2378	NN	denotes	phospholipid
T1235	2378-2379	HYPH	denotes	-
T1236	2379-2386	VBG	denotes	binding
T1237	2386-2388	,	denotes	,
T1238	2388-2391	DT	denotes	the
T1240	2392-2393	NN	denotes	N
T1242	2393-2394	HYPH	denotes	-
T1241	2394-2402	JJ	denotes	terminal
T1239	2403-2409	NN	denotes	domain
T1227	2410-2417	VBZ	denotes	appears
T1243	2418-2420	TO	denotes	to
T1244	2421-2427	VB	denotes	confer
T1245	2428-2438	JJ	denotes	functional
T1246	2439-2448	NN	denotes	diversity
T1247	2449-2450	-LRB-	denotes	[
T1248	2450-2451	CD	denotes	2
T1249	2451-2452	SYM	denotes	-
T1250	2452-2453	CD	denotes	4
T1251	2453-2454	-RRB-	denotes	]
T1252	2454-2455	.	denotes	.
T1253	2455-2543	sentence	denotes	The binding to negatively charged phospholipids is thought to be mediated by Ca2+ ions.
T1254	2456-2459	DT	denotes	The
T1255	2460-2467	NN	denotes	binding
T1257	2468-2470	IN	denotes	to
T1258	2471-2481	RB	denotes	negatively
T1259	2482-2489	JJ	denotes	charged
T1260	2490-2503	NNS	denotes	phospholipids
T1261	2504-2506	VBZ	denotes	is
T1256	2507-2514	VBN	denotes	thought
T1262	2515-2517	TO	denotes	to
T1264	2518-2520	VB	denotes	be
T1263	2521-2529	VBN	denotes	mediated
T1265	2530-2532	IN	denotes	by
T1266	2533-2537	NN	denotes	Ca2+
T1267	2538-2542	NNS	denotes	ions
T1268	2542-2543	.	denotes	.
T1269	2543-2670	sentence	denotes	Annexin A7 is unique in that it carries an extraordinarily long and hydrophobic amino terminus with more than 100 amino acids.
T1270	2544-2551	NN	denotes	Annexin
T1271	2552-2554	NN	denotes	A7
T1272	2555-2557	VBZ	denotes	is
T1273	2558-2564	JJ	denotes	unique
T1274	2565-2567	IN	denotes	in
T1276	2568-2572	IN	denotes	that
T1277	2573-2575	PRP	denotes	it
T1275	2576-2583	VBZ	denotes	carries
T1278	2584-2586	DT	denotes	an
T1280	2587-2602	RB	denotes	extraordinarily
T1281	2603-2607	JJ	denotes	long
T1282	2608-2611	CC	denotes	and
T1283	2612-2623	JJ	denotes	hydrophobic
T1284	2624-2629	NN	denotes	amino
T1279	2630-2638	NN	denotes	terminus
T1285	2639-2643	IN	denotes	with
T1286	2644-2648	JJR	denotes	more
T1288	2649-2653	IN	denotes	than
T1287	2654-2657	CD	denotes	100
T1290	2658-2663	NN	denotes	amino
T1289	2664-2669	NNS	denotes	acids
T1291	2669-2670	.	denotes	.
T1292	2670-2736	sentence	denotes	Alternative splicing gives rise to two isoforms of 47 and 51 kDa.
T1293	2671-2682	JJ	denotes	Alternative
T1294	2683-2691	NN	denotes	splicing
T1295	2692-2697	VBZ	denotes	gives
T1296	2698-2702	NN	denotes	rise
T1297	2703-2705	IN	denotes	to
T1298	2706-2709	CD	denotes	two
T1299	2710-2718	NNS	denotes	isoforms
T1300	2719-2721	IN	denotes	of
T1301	2722-2724	CD	denotes	47
T1303	2725-2728	CC	denotes	and
T1304	2729-2731	CD	denotes	51
T1302	2732-2735	NN	denotes	kDa
T1305	2735-2736	.	denotes	.
T1306	2736-2841	sentence	denotes	Both isoforms differ by an additional cassette exon located in the first third of the N-terminal domain.
T1307	2737-2741	DT	denotes	Both
T1308	2742-2750	NNS	denotes	isoforms
T1309	2751-2757	VBP	denotes	differ
T1310	2758-2760	IN	denotes	by
T1311	2761-2763	DT	denotes	an
T1313	2764-2774	JJ	denotes	additional
T1314	2775-2783	NN	denotes	cassette
T1312	2784-2788	NN	denotes	exon
T1315	2789-2796	JJ	denotes	located
T1316	2797-2799	IN	denotes	in
T1317	2800-2803	DT	denotes	the
T1319	2804-2809	JJ	denotes	first
T1318	2810-2815	NN	denotes	third
T1320	2816-2818	IN	denotes	of
T1321	2819-2822	DT	denotes	the
T1323	2823-2824	NN	denotes	N
T1325	2824-2825	HYPH	denotes	-
T1324	2825-2833	JJ	denotes	terminal
T1322	2834-2840	NN	denotes	domain
T1326	2840-2841	.	denotes	.
T1327	2841-3003	sentence	denotes	Most tissues harbor only the 47 kDa isoform, both forms are found in brain and heart, while the large isoform is exclusively expressed in mature skeletal muscle.
T1328	2842-2846	JJS	denotes	Most
T1329	2847-2854	NNS	denotes	tissues
T1330	2855-2861	VBP	denotes	harbor
T1332	2862-2866	RB	denotes	only
T1334	2867-2870	DT	denotes	the
T1335	2871-2873	CD	denotes	47
T1336	2874-2877	NN	denotes	kDa
T1333	2878-2885	NN	denotes	isoform
T1337	2885-2887	,	denotes	,
T1338	2887-2891	DT	denotes	both
T1339	2892-2897	NNS	denotes	forms
T1340	2898-2901	VBP	denotes	are
T1331	2902-2907	VBN	denotes	found
T1341	2908-2910	IN	denotes	in
T1342	2911-2916	NN	denotes	brain
T1343	2917-2920	CC	denotes	and
T1344	2921-2926	NN	denotes	heart
T1345	2926-2928	,	denotes	,
T1346	2928-2933	IN	denotes	while
T1348	2934-2937	DT	denotes	the
T1350	2938-2943	JJ	denotes	large
T1349	2944-2951	NN	denotes	isoform
T1351	2952-2954	VBZ	denotes	is
T1352	2955-2966	RB	denotes	exclusively
T1347	2967-2976	VBN	denotes	expressed
T1353	2977-2979	IN	denotes	in
T1354	2980-2986	JJ	denotes	mature
T1356	2987-2995	JJ	denotes	skeletal
T1355	2996-3002	NN	denotes	muscle
T1357	3002-3003	.	denotes	.
T1358	3003-3209	sentence	denotes	At the cellular level annexin A7 can be detected in the cytosol, at the plasma membrane, around the nucleus, at vesicular structures including adrenal chromaffin granules, and at the t-tubule system [5,6].
T1359	3004-3006	IN	denotes	At
T1361	3007-3010	DT	denotes	the
T1363	3011-3019	JJ	denotes	cellular
T1362	3020-3025	NN	denotes	level
T1364	3026-3033	NN	denotes	annexin
T1365	3034-3036	NN	denotes	A7
T1366	3037-3040	MD	denotes	can
T1367	3041-3043	VB	denotes	be
T1360	3044-3052	VBN	denotes	detected
T1368	3053-3055	IN	denotes	in
T1369	3056-3059	DT	denotes	the
T1370	3060-3067	NN	denotes	cytosol
T1371	3067-3069	,	denotes	,
T1372	3069-3071	IN	denotes	at
T1373	3072-3075	DT	denotes	the
T1375	3076-3082	NN	denotes	plasma
T1374	3083-3091	NN	denotes	membrane
T1376	3091-3093	,	denotes	,
T1377	3093-3099	IN	denotes	around
T1378	3100-3103	DT	denotes	the
T1379	3104-3111	NN	denotes	nucleus
T1380	3111-3113	,	denotes	,
T1381	3113-3115	IN	denotes	at
T1382	3116-3125	JJ	denotes	vesicular
T1383	3126-3136	NNS	denotes	structures
T1384	3137-3146	VBG	denotes	including
T1385	3147-3154	JJ	denotes	adrenal
T1387	3155-3165	NN	denotes	chromaffin
T1386	3166-3174	NNS	denotes	granules
T1388	3174-3176	,	denotes	,
T1389	3176-3179	CC	denotes	and
T1390	3180-3182	IN	denotes	at
T1391	3183-3186	DT	denotes	the
T1393	3187-3188	NN	denotes	t
T1395	3188-3189	HYPH	denotes	-
T1394	3189-3195	NN	denotes	tubule
T1392	3196-3202	NN	denotes	system
T1396	3203-3204	-LRB-	denotes	[
T1398	3204-3205	CD	denotes	5
T1399	3205-3206	,	denotes	,
T1397	3206-3207	CD	denotes	6
T1400	3207-3208	-RRB-	denotes	]
T1401	3208-3209	.	denotes	.
T1402	3209-3420	sentence	denotes	Annexin A7 translocates to membranes in a Ca2+-dependent fashion and, when intracellular Ca2+ levels rise, sequentially redistributes to the plasma and the nuclear membrane as well as to intracellular vesicles.
T1403	3210-3217	NN	denotes	Annexin
T1404	3218-3220	NN	denotes	A7
T1405	3221-3233	VBZ	denotes	translocates
T1406	3234-3236	IN	denotes	to
T1407	3237-3246	NNS	denotes	membranes
T1408	3247-3249	IN	denotes	in
T1409	3250-3251	DT	denotes	a
T1411	3252-3256	NN	denotes	Ca2+
T1413	3256-3257	HYPH	denotes	-
T1412	3257-3266	JJ	denotes	dependent
T1410	3267-3274	NN	denotes	fashion
T1414	3275-3278	CC	denotes	and
T1415	3278-3280	,	denotes	,
T1417	3280-3284	WRB	denotes	when
T1419	3285-3298	JJ	denotes	intracellular
T1421	3299-3303	NN	denotes	Ca2+
T1420	3304-3310	NNS	denotes	levels
T1418	3311-3315	VBP	denotes	rise
T1422	3315-3317	,	denotes	,
T1423	3317-3329	RB	denotes	sequentially
T1416	3330-3343	VBZ	denotes	redistributes
T1424	3344-3346	IN	denotes	to
T1425	3347-3350	DT	denotes	the
T1426	3351-3357	NN	denotes	plasma
T1427	3358-3361	CC	denotes	and
T1428	3362-3365	DT	denotes	the
T1430	3366-3373	JJ	denotes	nuclear
T1429	3374-3382	NN	denotes	membrane
T1431	3383-3385	RB	denotes	as
T1433	3386-3390	RB	denotes	well
T1432	3391-3393	IN	denotes	as
T1434	3394-3396	IN	denotes	to
T1435	3397-3410	JJ	denotes	intracellular
T1436	3411-3419	NNS	denotes	vesicles
T1437	3419-3420	.	denotes	.
T1438	3420-3477	sentence	denotes	Furthermore, annexin A7 associates with lipid rafts [7].
T1439	3421-3432	RB	denotes	Furthermore
T1441	3432-3434	,	denotes	,
T1442	3434-3441	NN	denotes	annexin
T1443	3442-3444	NN	denotes	A7
T1440	3445-3455	VBZ	denotes	associates
T1444	3456-3460	IN	denotes	with
T1445	3461-3466	NN	denotes	lipid
T1446	3467-3472	NNS	denotes	rafts
T1447	3473-3474	-LRB-	denotes	[
T1448	3474-3475	CD	denotes	7
T1449	3475-3476	-RRB-	denotes	]
T1450	3476-3477	.	denotes	.
T1451	3477-3592	sentence	denotes	Lipid rafts play a key role in membrane budding and in vesiculation processes such as endo- and exocytosis [8-10].
T1452	3478-3483	NN	denotes	Lipid
T1453	3484-3489	NNS	denotes	rafts
T1454	3490-3494	VBP	denotes	play
T1455	3495-3496	DT	denotes	a
T1457	3497-3500	JJ	denotes	key
T1456	3501-3505	NN	denotes	role
T1458	3506-3508	IN	denotes	in
T1459	3509-3517	NN	denotes	membrane
T1460	3518-3525	NN	denotes	budding
T1461	3526-3529	CC	denotes	and
T1462	3530-3532	IN	denotes	in
T1463	3533-3545	NN	denotes	vesiculation
T1464	3546-3555	NNS	denotes	processes
T1465	3556-3560	JJ	denotes	such
T1466	3561-3563	IN	denotes	as
T1467	3564-3568	AFX	denotes	endo
T1469	3568-3569	HYPH	denotes	-
T1470	3570-3573	CC	denotes	and
T1468	3574-3584	NN	denotes	exocytosis
T1471	3585-3586	-LRB-	denotes	[
T1472	3586-3587	CD	denotes	8
T1473	3587-3588	SYM	denotes	-
T1474	3588-3590	CD	denotes	10
T1475	3590-3591	-RRB-	denotes	]
T1476	3591-3592	.	denotes	.
T1477	3592-3680	sentence	denotes	Two binding partners of annexin A7 have been identified, sorcin and galectin-3 [11-13].
T1478	3593-3596	CD	denotes	Two
T1480	3597-3604	VBG	denotes	binding
T1479	3605-3613	NNS	denotes	partners
T1482	3614-3616	IN	denotes	of
T1483	3617-3624	NN	denotes	annexin
T1484	3625-3627	NN	denotes	A7
T1485	3628-3632	VBP	denotes	have
T1486	3633-3637	VBN	denotes	been
T1481	3638-3648	VBN	denotes	identified
T1487	3648-3650	,	denotes	,
T1488	3650-3656	NN	denotes	sorcin
T1489	3657-3660	CC	denotes	and
T1490	3661-3669	NN	denotes	galectin
T1491	3669-3670	HYPH	denotes	-
T1492	3670-3671	CD	denotes	3
T1493	3672-3673	-LRB-	denotes	[
T1494	3673-3675	CD	denotes	11
T1495	3675-3676	SYM	denotes	-
T1496	3676-3678	CD	denotes	13
T1497	3678-3679	-RRB-	denotes	]
T1498	3679-3680	.	denotes	.
T1499	3680-3767	sentence	denotes	Sorcin is a Ca2+-binding protein and belongs to the penta EF-hand protein family [14].
T1500	3681-3687	NN	denotes	Sorcin
T1501	3688-3690	VBZ	denotes	is
T1502	3691-3692	DT	denotes	a
T1504	3693-3697	NN	denotes	Ca2+
T1506	3697-3698	HYPH	denotes	-
T1505	3698-3705	VBG	denotes	binding
T1503	3706-3713	NN	denotes	protein
T1507	3714-3717	CC	denotes	and
T1508	3718-3725	VBZ	denotes	belongs
T1509	3726-3728	IN	denotes	to
T1510	3729-3732	DT	denotes	the
T1512	3733-3738	NN	denotes	penta
T1514	3739-3741	NN	denotes	EF
T1516	3741-3742	HYPH	denotes	-
T1515	3742-3746	NN	denotes	hand
T1513	3747-3754	NN	denotes	protein
T1511	3755-3761	NN	denotes	family
T1517	3762-3763	-LRB-	denotes	[
T1518	3763-3765	CD	denotes	14
T1519	3765-3766	-RRB-	denotes	]
T1520	3766-3767	.	denotes	.
T1521	3767-3833	sentence	denotes	Like annexin A7 it binds to membranes in a Ca2+-dependent manner.
T1522	3768-3772	IN	denotes	Like
T1524	3773-3780	NN	denotes	annexin
T1525	3781-3783	NN	denotes	A7
T1526	3784-3786	PRP	denotes	it
T1523	3787-3792	VBZ	denotes	binds
T1527	3793-3795	IN	denotes	to
T1528	3796-3805	NNS	denotes	membranes
T1529	3806-3808	IN	denotes	in
T1530	3809-3810	DT	denotes	a
T1532	3811-3815	NN	denotes	Ca2+
T1534	3815-3816	HYPH	denotes	-
T1533	3816-3825	JJ	denotes	dependent
T1531	3826-3832	NN	denotes	manner
T1535	3832-3833	.	denotes	.
T1536	3833-3957	sentence	denotes	Sorcin also has been described as interaction partner of the ryanodine receptor, and appears to modulate its function [15].
T1537	3834-3840	NN	denotes	Sorcin
T1539	3841-3845	RB	denotes	also
T1540	3846-3849	VBZ	denotes	has
T1541	3850-3854	VBN	denotes	been
T1538	3855-3864	VBN	denotes	described
T1542	3865-3867	IN	denotes	as
T1543	3868-3879	NN	denotes	interaction
T1544	3880-3887	NN	denotes	partner
T1545	3888-3890	IN	denotes	of
T1546	3891-3894	DT	denotes	the
T1548	3895-3904	NN	denotes	ryanodine
T1547	3905-3913	NN	denotes	receptor
T1549	3913-3915	,	denotes	,
T1550	3915-3918	CC	denotes	and
T1551	3919-3926	VBZ	denotes	appears
T1552	3927-3929	TO	denotes	to
T1553	3930-3938	VB	denotes	modulate
T1554	3939-3942	PRP$	denotes	its
T1555	3943-3951	NN	denotes	function
T1556	3952-3953	-LRB-	denotes	[
T1557	3953-3955	CD	denotes	15
T1558	3955-3956	-RRB-	denotes	]
T1559	3956-3957	.	denotes	.
T1560	3957-4046	sentence	denotes	The influence of the sorcin/annexin A7 interaction on the ryanodine receptor is unknown.
T1561	3958-3961	DT	denotes	The
T1562	3962-3971	NN	denotes	influence
T1564	3972-3974	IN	denotes	of
T1565	3975-3978	DT	denotes	the
T1567	3979-3985	NN	denotes	sorcin
T1569	3985-3986	HYPH	denotes	/
T1568	3986-3993	NN	denotes	annexin
T1570	3994-3996	NN	denotes	A7
T1566	3997-4008	NN	denotes	interaction
T1571	4009-4011	IN	denotes	on
T1572	4012-4015	DT	denotes	the
T1574	4016-4025	NN	denotes	ryanodine
T1573	4026-4034	NN	denotes	receptor
T1563	4035-4037	VBZ	denotes	is
T1575	4038-4045	JJ	denotes	unknown
T1576	4045-4046	.	denotes	.
T1577	4046-4121	sentence	denotes	Sorcin and annexin A7 are coexpressed in all tissues examined so far [11].
T1578	4047-4053	NN	denotes	Sorcin
T1580	4054-4057	CC	denotes	and
T1581	4058-4065	NN	denotes	annexin
T1582	4066-4068	NN	denotes	A7
T1583	4069-4072	VBP	denotes	are
T1579	4073-4084	VBN	denotes	coexpressed
T1584	4085-4087	IN	denotes	in
T1585	4088-4091	DT	denotes	all
T1586	4092-4099	NNS	denotes	tissues
T1587	4100-4108	VBN	denotes	examined
T1588	4109-4111	RB	denotes	so
T1589	4112-4115	RB	denotes	far
T1590	4116-4117	-LRB-	denotes	[
T1591	4117-4119	CD	denotes	11
T1592	4119-4120	-RRB-	denotes	]
T1593	4120-4121	.	denotes	.
T1594	4121-4222	sentence	denotes	The binding of annexin A7 and sorcin is Ca2+-dependent and occurs at micromolar Ca2+ concentrations.
T1595	4122-4125	DT	denotes	The
T1596	4126-4133	NN	denotes	binding
T1598	4134-4136	IN	denotes	of
T1599	4137-4144	NN	denotes	annexin
T1600	4145-4147	NN	denotes	A7
T1601	4148-4151	CC	denotes	and
T1602	4152-4158	NN	denotes	sorcin
T1597	4159-4161	VBZ	denotes	is
T1603	4162-4166	NN	denotes	Ca2+
T1605	4166-4167	HYPH	denotes	-
T1604	4167-4176	JJ	denotes	dependent
T1606	4177-4180	CC	denotes	and
T1607	4181-4187	VBZ	denotes	occurs
T1608	4188-4190	IN	denotes	at
T1609	4191-4201	JJ	denotes	micromolar
T1610	4202-4206	NN	denotes	Ca2+
T1611	4207-4221	NNS	denotes	concentrations
T1612	4221-4222	.	denotes	.
T1613	4222-4372	sentence	denotes	The binding sites have been localised to the amino terminal GGYY and GYGG motifs in sorcin and to the GYPP motif in the amino terminus of annexin A7.
T1614	4223-4226	DT	denotes	The
T1616	4227-4234	VBG	denotes	binding
T1615	4235-4240	NNS	denotes	sites
T1618	4241-4245	VBP	denotes	have
T1619	4246-4250	VBN	denotes	been
T1617	4251-4260	VBN	denotes	localised
T1620	4261-4263	IN	denotes	to
T1621	4264-4267	DT	denotes	the
T1623	4268-4273	NN	denotes	amino
T1624	4274-4282	JJ	denotes	terminal
T1625	4283-4287	NN	denotes	GGYY
T1626	4288-4291	CC	denotes	and
T1627	4292-4296	NN	denotes	GYGG
T1622	4297-4303	NNS	denotes	motifs
T1628	4304-4306	IN	denotes	in
T1629	4307-4313	NN	denotes	sorcin
T1630	4314-4317	CC	denotes	and
T1631	4318-4320	IN	denotes	to
T1632	4321-4324	DT	denotes	the
T1634	4325-4329	NN	denotes	GYPP
T1633	4330-4335	NN	denotes	motif
T1635	4336-4338	IN	denotes	in
T1636	4339-4342	DT	denotes	the
T1638	4343-4348	NN	denotes	amino
T1637	4349-4357	NN	denotes	terminus
T1639	4358-4360	IN	denotes	of
T1640	4361-4368	NN	denotes	annexin
T1641	4369-4371	NN	denotes	A7
T1642	4371-4372	.	denotes	.
T1643	4372-4479	sentence	denotes	The proteins bind to each other with a stoichiometry of two sorcin molecules per annexin A7 molecule [12].
T1644	4373-4376	DT	denotes	The
T1645	4377-4385	NN	denotes	proteins
T1646	4386-4390	VBP	denotes	bind
T1647	4391-4393	IN	denotes	to
T1648	4394-4398	DT	denotes	each
T1649	4399-4404	JJ	denotes	other
T1650	4405-4409	IN	denotes	with
T1651	4410-4411	DT	denotes	a
T1652	4412-4425	NN	denotes	stoichiometry
T1653	4426-4428	IN	denotes	of
T1654	4429-4432	CD	denotes	two
T1656	4433-4439	NN	denotes	sorcin
T1655	4440-4449	NNS	denotes	molecules
T1657	4450-4453	IN	denotes	per
T1658	4454-4461	NN	denotes	annexin
T1659	4462-4464	NN	denotes	A7
T1660	4465-4473	NN	denotes	molecule
T1661	4474-4475	-LRB-	denotes	[
T1662	4475-4477	CD	denotes	12
T1663	4477-4478	-RRB-	denotes	]
T1664	4478-4479	.	denotes	.
T1665	4479-4645	sentence	denotes	Galectin-3 is a multifunctional oncogenic protein with an anti-apoptotic activity found in the extracellular space, in the nucleus and cytoplasm and in mitochondria.
T1666	4480-4488	NN	denotes	Galectin
T1668	4488-4489	HYPH	denotes	-
T1669	4489-4490	CD	denotes	3
T1667	4491-4493	VBZ	denotes	is
T1670	4494-4495	DT	denotes	a
T1672	4496-4511	JJ	denotes	multifunctional
T1673	4512-4521	JJ	denotes	oncogenic
T1671	4522-4529	NN	denotes	protein
T1674	4530-4534	IN	denotes	with
T1675	4535-4537	DT	denotes	an
T1677	4538-4552	JJ	denotes	anti-apoptotic
T1676	4553-4561	NN	denotes	activity
T1678	4562-4567	VBN	denotes	found
T1679	4568-4570	IN	denotes	in
T1680	4571-4574	DT	denotes	the
T1682	4575-4588	JJ	denotes	extracellular
T1681	4589-4594	NN	denotes	space
T1683	4594-4596	,	denotes	,
T1684	4596-4598	IN	denotes	in
T1685	4599-4602	DT	denotes	the
T1686	4603-4610	NN	denotes	nucleus
T1687	4611-4614	CC	denotes	and
T1688	4615-4624	NN	denotes	cytoplasm
T1689	4625-4628	CC	denotes	and
T1690	4629-4631	IN	denotes	in
T1691	4632-4644	NNS	denotes	mitochondria
T1692	4644-4645	.	denotes	.
T1693	4645-4740	sentence	denotes	Cytoplasmic galectin-3 correlates with tumor progression and protects mitochondrial integrity.
T1694	4646-4657	JJ	denotes	Cytoplasmic
T1695	4658-4666	NN	denotes	galectin
T1697	4666-4667	HYPH	denotes	-
T1698	4667-4668	CD	denotes	3
T1696	4669-4679	VBZ	denotes	correlates
T1699	4680-4684	IN	denotes	with
T1700	4685-4690	NN	denotes	tumor
T1701	4691-4702	NN	denotes	progression
T1702	4703-4706	CC	denotes	and
T1703	4707-4715	VBZ	denotes	protects
T1704	4716-4729	JJ	denotes	mitochondrial
T1705	4730-4739	NN	denotes	integrity
T1706	4739-4740	.	denotes	.
T1707	4740-4868	sentence	denotes	Down regulation of annexin A7 prevents galectin-3 translocation to the perinuclear membrane and increases galectin-3 secretion.
T1708	4741-4745	NN	denotes	Down
T1709	4746-4756	NN	denotes	regulation
T1711	4757-4759	IN	denotes	of
T1712	4760-4767	NN	denotes	annexin
T1713	4768-4770	NN	denotes	A7
T1710	4771-4779	VBZ	denotes	prevents
T1714	4780-4788	NN	denotes	galectin
T1716	4788-4789	HYPH	denotes	-
T1715	4789-4790	NN	denotes	3
T1717	4791-4804	NN	denotes	translocation
T1718	4805-4807	IN	denotes	to
T1719	4808-4811	DT	denotes	the
T1721	4812-4823	JJ	denotes	perinuclear
T1720	4824-4832	NN	denotes	membrane
T1722	4833-4836	CC	denotes	and
T1723	4837-4846	VBZ	denotes	increases
T1724	4847-4855	NN	denotes	galectin
T1726	4855-4856	HYPH	denotes	-
T1725	4856-4857	NN	denotes	3
T1727	4858-4867	NN	denotes	secretion
T1728	4867-4868	.	denotes	.
T1729	4868-4987	sentence	denotes	For annexin A7 a role for galectin-3 trafficking, apoptosis regulation, and mitochondrial integrity was proposed [13].
T1730	4869-4872	IN	denotes	For
T1732	4873-4880	NN	denotes	annexin
T1733	4881-4883	NN	denotes	A7
T1734	4884-4885	DT	denotes	a
T1735	4886-4890	NN	denotes	role
T1736	4891-4894	IN	denotes	for
T1737	4895-4903	NN	denotes	galectin
T1739	4903-4904	HYPH	denotes	-
T1738	4904-4905	NN	denotes	3
T1740	4906-4917	NN	denotes	trafficking
T1741	4917-4919	,	denotes	,
T1742	4919-4928	NN	denotes	apoptosis
T1743	4929-4939	NN	denotes	regulation
T1744	4939-4941	,	denotes	,
T1745	4941-4944	CC	denotes	and
T1746	4945-4958	JJ	denotes	mitochondrial
T1747	4959-4968	NN	denotes	integrity
T1748	4969-4972	VBD	denotes	was
T1731	4973-4981	VBN	denotes	proposed
T1749	4982-4983	-LRB-	denotes	[
T1750	4983-4985	CD	denotes	13
T1751	4985-4986	-RRB-	denotes	]
T1752	4986-4987	.	denotes	.
T1753	4987-5043	sentence	denotes	The cellular role of annexin A7 is not well understood.
T1754	4988-4991	DT	denotes	The
T1756	4992-5000	JJ	denotes	cellular
T1755	5001-5005	NN	denotes	role
T1758	5006-5008	IN	denotes	of
T1759	5009-5016	NN	denotes	annexin
T1760	5017-5019	NN	denotes	A7
T1761	5020-5022	VBZ	denotes	is
T1762	5023-5026	RB	denotes	not
T1763	5027-5031	RB	denotes	well
T1757	5032-5042	VBN	denotes	understood
T1764	5042-5043	.	denotes	.
T1765	5043-5178	sentence	denotes	It is thought to regulate and stabilize membrane domains and to have a role in Ca2+ homeostasis and Ca2+-dependent signaling pathways.
T1766	5044-5046	PRP	denotes	It
T1768	5047-5049	VBZ	denotes	is
T1767	5050-5057	VBN	denotes	thought
T1769	5058-5060	TO	denotes	to
T1770	5061-5069	VB	denotes	regulate
T1771	5070-5073	CC	denotes	and
T1772	5074-5083	VB	denotes	stabilize
T1773	5084-5092	NN	denotes	membrane
T1774	5093-5100	NNS	denotes	domains
T1775	5101-5104	CC	denotes	and
T1776	5105-5107	TO	denotes	to
T1777	5108-5112	VB	denotes	have
T1778	5113-5114	DT	denotes	a
T1779	5115-5119	NN	denotes	role
T1780	5120-5122	IN	denotes	in
T1781	5123-5127	NN	denotes	Ca2+
T1782	5128-5139	NN	denotes	homeostasis
T1783	5140-5143	CC	denotes	and
T1784	5144-5148	NN	denotes	Ca2+
T1786	5148-5149	HYPH	denotes	-
T1785	5149-5158	JJ	denotes	dependent
T1788	5159-5168	NN	denotes	signaling
T1787	5169-5177	NNS	denotes	pathways
T1789	5177-5178	.	denotes	.
T1790	5178-5278	sentence	denotes	These proposals are supported by data obtained from analysis of annexin A7 deficient mouse mutants.
T1791	5179-5184	DT	denotes	These
T1792	5185-5194	NNS	denotes	proposals
T1794	5195-5198	VBP	denotes	are
T1793	5199-5208	VBN	denotes	supported
T1795	5209-5211	IN	denotes	by
T1796	5212-5216	NNS	denotes	data
T1797	5217-5225	VBN	denotes	obtained
T1798	5226-5230	IN	denotes	from
T1799	5231-5239	NN	denotes	analysis
T1800	5240-5242	IN	denotes	of
T1801	5243-5250	NN	denotes	annexin
T1802	5251-5253	NN	denotes	A7
T1803	5254-5263	JJ	denotes	deficient
T1805	5264-5269	NN	denotes	mouse
T1804	5270-5277	NNS	denotes	mutants
T1806	5277-5278	.	denotes	.
T1807	5278-5374	sentence	denotes	Two annexin A7 129Sv null mice strains were generated independently using a different strategy.
T1808	5279-5282	CD	denotes	Two
T1810	5283-5290	NN	denotes	annexin
T1811	5291-5293	NN	denotes	A7
T1812	5294-5299	NN	denotes	129Sv
T1813	5300-5304	JJ	denotes	null
T1814	5305-5309	NNS	denotes	mice
T1809	5310-5317	NNS	denotes	strains
T1816	5318-5322	VBD	denotes	were
T1815	5323-5332	VBN	denotes	generated
T1817	5333-5346	RB	denotes	independently
T1818	5347-5352	VBG	denotes	using
T1819	5353-5354	DT	denotes	a
T1821	5355-5364	JJ	denotes	different
T1820	5365-5373	NN	denotes	strategy
T1822	5373-5374	.	denotes	.
T1823	5374-5419	sentence	denotes	The one of Srivastava et al. [16] is lethal.
T1824	5375-5378	DT	denotes	The
T1825	5379-5382	CD	denotes	one
T1827	5383-5385	IN	denotes	of
T1828	5386-5396	NNP	denotes	Srivastava
T1829	5397-5399	FW	denotes	et
T1830	5400-5403	FW	denotes	al.
T1831	5404-5405	-LRB-	denotes	[
T1832	5405-5407	CD	denotes	16
T1833	5407-5408	-RRB-	denotes	]
T1826	5409-5411	VBZ	denotes	is
T1834	5412-5418	JJ	denotes	lethal
T1835	5418-5419	.	denotes	.
T1836	5419-5495	sentence	denotes	Heterozygous mice exhibit an insulin secretion defect and tumor phenotypes.
T1837	5420-5432	JJ	denotes	Heterozygous
T1838	5433-5437	NNS	denotes	mice
T1839	5438-5445	VBP	denotes	exhibit
T1840	5446-5448	DT	denotes	an
T1842	5449-5456	NN	denotes	insulin
T1843	5457-5466	NN	denotes	secretion
T1841	5467-5473	NN	denotes	defect
T1844	5474-5477	CC	denotes	and
T1845	5478-5483	NN	denotes	tumor
T1846	5484-5494	NNS	denotes	phenotypes
T1847	5494-5495	.	denotes	.
T1848	5495-5624	sentence	denotes	The null strain reported by Herr et al. [17] is viable, healthy, and shows no insulin secretion defect or other obvious defects.
T1849	5496-5499	DT	denotes	The
T1851	5500-5504	JJ	denotes	null
T1850	5505-5511	NN	denotes	strain
T1853	5512-5520	VBN	denotes	reported
T1854	5521-5523	IN	denotes	by
T1855	5524-5528	NNP	denotes	Herr
T1856	5529-5531	FW	denotes	et
T1857	5532-5535	FW	denotes	al.
T1858	5536-5537	-LRB-	denotes	[
T1859	5537-5539	CD	denotes	17
T1860	5539-5540	-RRB-	denotes	]
T1852	5541-5543	VBZ	denotes	is
T1861	5544-5550	JJ	denotes	viable
T1863	5550-5552	,	denotes	,
T1862	5552-5559	JJ	denotes	healthy
T1864	5559-5561	,	denotes	,
T1865	5561-5564	CC	denotes	and
T1866	5565-5570	VBZ	denotes	shows
T1867	5571-5573	DT	denotes	no
T1869	5574-5581	NN	denotes	insulin
T1870	5582-5591	NN	denotes	secretion
T1868	5592-5598	NN	denotes	defect
T1871	5599-5601	CC	denotes	or
T1872	5602-5607	JJ	denotes	other
T1874	5608-5615	JJ	denotes	obvious
T1873	5616-5623	NNS	denotes	defects
T1875	5623-5624	.	denotes	.
T1876	5624-5707	sentence	denotes	However, in isolated cardiomyocytes the frequency-induced shortening is disturbed.
T1877	5625-5632	RB	denotes	However
T1879	5632-5634	,	denotes	,
T1880	5634-5636	IN	denotes	in
T1881	5637-5645	VBN	denotes	isolated
T1882	5646-5660	NNS	denotes	cardiomyocytes
T1883	5661-5664	DT	denotes	the
T1885	5665-5674	NN	denotes	frequency
T1887	5674-5675	HYPH	denotes	-
T1886	5675-5682	VBN	denotes	induced
T1884	5683-5693	NN	denotes	shortening
T1888	5694-5696	VBZ	denotes	is
T1878	5697-5706	VBN	denotes	disturbed
T1889	5706-5707	.	denotes	.
T1890	5707-5801	sentence	denotes	Here we have focused on the analysis of red blood cells and platelets of the anxA7-/- mutant.
T1891	5708-5712	RB	denotes	Here
T1893	5713-5715	PRP	denotes	we
T1894	5716-5720	VBP	denotes	have
T1892	5721-5728	VBN	denotes	focused
T1895	5729-5731	IN	denotes	on
T1896	5732-5735	DT	denotes	the
T1897	5736-5744	NN	denotes	analysis
T1898	5745-5747	IN	denotes	of
T1899	5748-5751	JJ	denotes	red
T1901	5752-5757	NN	denotes	blood
T1900	5758-5763	NNS	denotes	cells
T1902	5764-5767	CC	denotes	and
T1903	5768-5777	NNS	denotes	platelets
T1904	5778-5780	IN	denotes	of
T1905	5781-5784	DT	denotes	the
T1907	5785-5790	JJ	denotes	anxA7
T1908	5790-5791	SYM	denotes	-
T1909	5791-5792	HYPH	denotes	/
T1910	5792-5793	SYM	denotes	-
T1906	5794-5800	NN	denotes	mutant
T1911	5800-5801	.	denotes	.
T1912	5801-5973	sentence	denotes	Generally, red blood cells and platelets were thought not to contain annexin A7 and only recently the 47 kDa isoform has been reported as component of red blood cells [7].
T1913	5802-5811	RB	denotes	Generally
T1915	5811-5813	,	denotes	,
T1916	5813-5816	JJ	denotes	red
T1918	5817-5822	NN	denotes	blood
T1917	5823-5828	NNS	denotes	cells
T1919	5829-5832	CC	denotes	and
T1920	5833-5842	NNS	denotes	platelets
T1921	5843-5847	VBD	denotes	were
T1914	5848-5855	VBN	denotes	thought
T1922	5856-5859	RB	denotes	not
T1923	5860-5862	TO	denotes	to
T1924	5863-5870	VB	denotes	contain
T1925	5871-5878	NN	denotes	annexin
T1926	5879-5881	NN	denotes	A7
T1927	5882-5885	CC	denotes	and
T1928	5886-5890	RB	denotes	only
T1929	5891-5899	RB	denotes	recently
T1931	5900-5903	DT	denotes	the
T1933	5904-5906	CD	denotes	47
T1934	5907-5910	NN	denotes	kDa
T1932	5911-5918	NN	denotes	isoform
T1935	5919-5922	VBZ	denotes	has
T1936	5923-5927	VBN	denotes	been
T1930	5928-5936	VBN	denotes	reported
T1937	5937-5939	IN	denotes	as
T1938	5940-5949	NN	denotes	component
T1939	5950-5952	IN	denotes	of
T1940	5953-5956	JJ	denotes	red
T1942	5957-5962	NN	denotes	blood
T1941	5963-5968	NNS	denotes	cells
T1943	5969-5970	-LRB-	denotes	[
T1944	5970-5971	CD	denotes	7
T1945	5971-5972	-RRB-	denotes	]
T1946	5972-5973	.	denotes	.
T1947	5973-6094	sentence	denotes	Red blood cells undergo various biochemical or morphological changes that appear to be Ca2+-dependent processes [18,19].
T1948	5974-5977	JJ	denotes	Red
T1950	5978-5983	NN	denotes	blood
T1949	5984-5989	NNS	denotes	cells
T1951	5990-5997	VBP	denotes	undergo
T1952	5998-6005	JJ	denotes	various
T1954	6006-6017	JJ	denotes	biochemical
T1955	6018-6020	CC	denotes	or
T1956	6021-6034	JJ	denotes	morphological
T1953	6035-6042	NNS	denotes	changes
T1957	6043-6047	WDT	denotes	that
T1958	6048-6054	VBP	denotes	appear
T1959	6055-6057	TO	denotes	to
T1960	6058-6060	VB	denotes	be
T1961	6061-6065	NN	denotes	Ca2+
T1963	6065-6066	HYPH	denotes	-
T1962	6066-6075	JJ	denotes	dependent
T1964	6076-6085	NNS	denotes	processes
T1965	6086-6087	-LRB-	denotes	[
T1967	6087-6089	CD	denotes	18
T1968	6089-6090	,	denotes	,
T1966	6090-6092	CD	denotes	19
T1969	6092-6093	-RRB-	denotes	]
T1970	6093-6094	.	denotes	.
T1971	6094-6204	sentence	denotes	One of them is the release of hemoglobin-containing exovesicles occurring in vivo as well as in vitro [7,20].
T1972	6095-6098	CD	denotes	One
T1974	6099-6101	IN	denotes	of
T1975	6102-6106	PRP	denotes	them
T1973	6107-6109	VBZ	denotes	is
T1976	6110-6113	DT	denotes	the
T1977	6114-6121	NN	denotes	release
T1978	6122-6124	IN	denotes	of
T1979	6125-6135	NN	denotes	hemoglobin
T1981	6135-6136	HYPH	denotes	-
T1980	6136-6146	VBG	denotes	containing
T1982	6147-6158	NNS	denotes	exovesicles
T1983	6159-6168	VBG	denotes	occurring
T1984	6169-6171	FW	denotes	in
T1985	6172-6176	FW	denotes	vivo
T1986	6177-6179	RB	denotes	as
T1988	6180-6184	RB	denotes	well
T1987	6185-6187	IN	denotes	as
T1989	6188-6190	FW	denotes	in
T1990	6191-6196	FW	denotes	vitro
T1991	6197-6198	-LRB-	denotes	[
T1993	6198-6199	CD	denotes	7
T1994	6199-6200	,	denotes	,
T1992	6200-6202	CD	denotes	20
T1995	6202-6203	-RRB-	denotes	]
T1996	6203-6204	.	denotes	.
T1997	6204-6339	sentence	denotes	This process is thought to represent a protective method of the red blood cell against an attack by for example complement components.
T1998	6205-6209	DT	denotes	This
T1999	6210-6217	NN	denotes	process
T2001	6218-6220	VBZ	denotes	is
T2000	6221-6228	VBN	denotes	thought
T2002	6229-6231	TO	denotes	to
T2003	6232-6241	VB	denotes	represent
T2004	6242-6243	DT	denotes	a
T2006	6244-6254	JJ	denotes	protective
T2005	6255-6261	NN	denotes	method
T2007	6262-6264	IN	denotes	of
T2008	6265-6268	DT	denotes	the
T2010	6269-6272	JJ	denotes	red
T2011	6273-6278	NN	denotes	blood
T2009	6279-6283	NN	denotes	cell
T2012	6284-6291	IN	denotes	against
T2013	6292-6294	DT	denotes	an
T2014	6295-6301	NN	denotes	attack
T2015	6302-6304	IN	denotes	by
T2016	6305-6308	IN	denotes	for
T2018	6309-6316	NN	denotes	example
T2019	6317-6327	NN	denotes	complement
T2017	6328-6338	NNS	denotes	components
T2020	6338-6339	.	denotes	.
T2021	6339-6466	sentence	denotes	Red blood cells which lack the ability to vesiculate cause a disease with red blood cell destruction and haemoglobinuria [21].
T2022	6340-6343	JJ	denotes	Red
T2024	6344-6349	NN	denotes	blood
T2023	6350-6355	NNS	denotes	cells
T2026	6356-6361	WDT	denotes	which
T2027	6362-6366	VBP	denotes	lack
T2028	6367-6370	DT	denotes	the
T2029	6371-6378	NN	denotes	ability
T2030	6379-6381	TO	denotes	to
T2031	6382-6392	VB	denotes	vesiculate
T2025	6393-6398	VB	denotes	cause
T2032	6399-6400	DT	denotes	a
T2033	6401-6408	NN	denotes	disease
T2034	6409-6413	IN	denotes	with
T2035	6414-6417	JJ	denotes	red
T2037	6418-6423	NN	denotes	blood
T2036	6424-6428	NN	denotes	cell
T2038	6429-6440	NN	denotes	destruction
T2039	6441-6444	CC	denotes	and
T2040	6445-6460	NN	denotes	haemoglobinuria
T2041	6461-6462	-LRB-	denotes	[
T2042	6462-6464	CD	denotes	21
T2043	6464-6465	-RRB-	denotes	]
T2044	6465-6466	.	denotes	.
T2045	6466-6572	sentence	denotes	There exist two types of vesicles, micro- and nanovesicles with a size of 180 nm and 60 nm, respectively.
T2046	6467-6472	EX	denotes	There
T2047	6473-6478	VBP	denotes	exist
T2048	6479-6482	CD	denotes	two
T2049	6483-6488	NNS	denotes	types
T2050	6489-6491	IN	denotes	of
T2051	6492-6500	NNS	denotes	vesicles
T2052	6500-6502	,	denotes	,
T2053	6502-6507	AFX	denotes	micro
T2055	6507-6508	HYPH	denotes	-
T2056	6509-6512	CC	denotes	and
T2054	6513-6525	NNS	denotes	nanovesicles
T2057	6526-6530	IN	denotes	with
T2058	6531-6532	DT	denotes	a
T2059	6533-6537	NN	denotes	size
T2060	6538-6540	IN	denotes	of
T2061	6541-6544	CD	denotes	180
T2062	6545-6547	NN	denotes	nm
T2063	6548-6551	CC	denotes	and
T2064	6552-6554	CD	denotes	60
T2065	6555-6557	NN	denotes	nm
T2066	6557-6559	,	denotes	,
T2067	6559-6571	RB	denotes	respectively
T2068	6571-6572	.	denotes	.
T2069	6572-6858	sentence	denotes	They are enriched in cholesterol and sphingolipid rich lipid raft domains that are associated with proteins like acetylcholinesterase, cell surface proteins including a complement receptor, and the lipid raft proteins stomatin and flotillin, but they lack any cytoskeletal protein [7].
T2070	6573-6577	PRP	denotes	They
T2072	6578-6581	VBP	denotes	are
T2071	6582-6590	VBN	denotes	enriched
T2073	6591-6593	IN	denotes	in
T2074	6594-6605	NN	denotes	cholesterol
T2076	6606-6609	CC	denotes	and
T2077	6610-6622	NN	denotes	sphingolipid
T2075	6623-6627	JJ	denotes	rich
T2079	6628-6633	NN	denotes	lipid
T2080	6634-6638	NN	denotes	raft
T2078	6639-6646	NNS	denotes	domains
T2081	6647-6651	WDT	denotes	that
T2083	6652-6655	VBP	denotes	are
T2082	6656-6666	VBN	denotes	associated
T2084	6667-6671	IN	denotes	with
T2085	6672-6680	NN	denotes	proteins
T2086	6681-6685	IN	denotes	like
T2087	6686-6706	NN	denotes	acetylcholinesterase
T2088	6706-6708	,	denotes	,
T2089	6708-6712	NN	denotes	cell
T2091	6713-6720	NN	denotes	surface
T2090	6721-6729	NN	denotes	proteins
T2092	6730-6739	VBG	denotes	including
T2093	6740-6741	DT	denotes	a
T2095	6742-6752	NN	denotes	complement
T2094	6753-6761	NN	denotes	receptor
T2096	6761-6763	,	denotes	,
T2097	6763-6766	CC	denotes	and
T2098	6767-6770	DT	denotes	the
T2100	6771-6776	NN	denotes	lipid
T2101	6777-6781	NN	denotes	raft
T2099	6782-6790	NN	denotes	proteins
T2102	6791-6799	NN	denotes	stomatin
T2103	6800-6803	CC	denotes	and
T2104	6804-6813	NN	denotes	flotillin
T2105	6813-6815	,	denotes	,
T2106	6815-6818	CC	denotes	but
T2107	6819-6823	PRP	denotes	they
T2108	6824-6828	VBP	denotes	lack
T2109	6829-6832	DT	denotes	any
T2111	6833-6845	JJ	denotes	cytoskeletal
T2110	6846-6853	NN	denotes	protein
T2112	6854-6855	-LRB-	denotes	[
T2113	6855-6856	CD	denotes	7
T2114	6856-6857	-RRB-	denotes	]
T2115	6857-6858	.	denotes	.
T2116	6858-6935	sentence	denotes	Furthermore, they contain sorcin and annexin A7 attached to the lipid rafts.
T2117	6859-6870	RB	denotes	Furthermore
T2119	6870-6872	,	denotes	,
T2120	6872-6876	PRP	denotes	they
T2118	6877-6884	VBP	denotes	contain
T2121	6885-6891	NN	denotes	sorcin
T2122	6892-6895	CC	denotes	and
T2123	6896-6903	NN	denotes	annexin
T2124	6904-6906	NN	denotes	A7
T2125	6907-6915	VBN	denotes	attached
T2126	6916-6918	IN	denotes	to
T2127	6919-6922	DT	denotes	the
T2129	6923-6928	NN	denotes	lipid
T2128	6929-6934	NNS	denotes	rafts
T2130	6934-6935	.	denotes	.
T2131	6935-6984	sentence	denotes	Both proteins are more abundant in nanovesicles.
T2132	6936-6940	DT	denotes	Both
T2133	6941-6949	NN	denotes	proteins
T2134	6950-6953	VBP	denotes	are
T2135	6954-6958	RBR	denotes	more
T2136	6959-6967	JJ	denotes	abundant
T2137	6968-6970	IN	denotes	in
T2138	6971-6983	NNS	denotes	nanovesicles
T2139	6983-6984	.	denotes	.
T2140	6984-7169	sentence	denotes	The vesicle formation goes along with several other changes in the red blood cell like cytoskeletal rearrangements and changes in the phospholipid orientation in the cellular membrane.
T2141	6985-6988	DT	denotes	The
T2143	6989-6996	NN	denotes	vesicle
T2142	6997-7006	NN	denotes	formation
T2144	7007-7011	VBZ	denotes	goes
T2145	7012-7017	RP	denotes	along
T2146	7018-7022	IN	denotes	with
T2147	7023-7030	JJ	denotes	several
T2149	7031-7036	JJ	denotes	other
T2148	7037-7044	NNS	denotes	changes
T2150	7045-7047	IN	denotes	in
T2151	7048-7051	DT	denotes	the
T2153	7052-7055	JJ	denotes	red
T2154	7056-7061	NN	denotes	blood
T2152	7062-7066	NN	denotes	cell
T2155	7067-7071	IN	denotes	like
T2156	7072-7084	JJ	denotes	cytoskeletal
T2157	7085-7099	NNS	denotes	rearrangements
T2158	7100-7103	CC	denotes	and
T2159	7104-7111	NNS	denotes	changes
T2160	7112-7114	IN	denotes	in
T2161	7115-7118	DT	denotes	the
T2163	7119-7131	NN	denotes	phospholipid
T2162	7132-7143	NN	denotes	orientation
T2164	7144-7146	IN	denotes	in
T2165	7147-7150	DT	denotes	the
T2167	7151-7159	JJ	denotes	cellular
T2166	7160-7168	NN	denotes	membrane
T2168	7168-7169	.	denotes	.
T2443	7180-7187	NN	denotes	Annexin
T2444	7188-7190	NN	denotes	A7
T2445	7191-7201	NN	denotes	expression
T2446	7202-7204	IN	denotes	in
T2447	7205-7208	JJ	denotes	red
T2449	7209-7214	NN	denotes	blood
T2448	7215-7220	NNS	denotes	cells
T2450	7220-7222	,	denotes	,
T2451	7222-7225	JJ	denotes	red
T2453	7226-7231	NN	denotes	blood
T2452	7232-7236	NN	denotes	cell
T2454	7237-7244	VBN	denotes	derived
T2455	7245-7256	NNS	denotes	exovesicles
T2456	7257-7260	CC	denotes	and
T2457	7261-7263	IN	denotes	in
T2458	7264-7273	NNS	denotes	platelets
T2459	7273-7514	sentence	denotes	Salzer et al. recently reported the presence of the 47 kDa isoform of annexin A7 and its partner sorcin in micro- and nanovesicles derived from red blood cells where they are located in the lumen and are also enriched in membrane rafts [7].
T2460	7274-7280	NNP	denotes	Salzer
T2462	7281-7283	FW	denotes	et
T2463	7284-7287	FW	denotes	al.
T2464	7288-7296	RB	denotes	recently
T2461	7297-7305	VBD	denotes	reported
T2465	7306-7309	DT	denotes	the
T2466	7310-7318	NN	denotes	presence
T2467	7319-7321	IN	denotes	of
T2468	7322-7325	DT	denotes	the
T2470	7326-7328	CD	denotes	47
T2471	7329-7332	NN	denotes	kDa
T2469	7333-7340	NN	denotes	isoform
T2472	7341-7343	IN	denotes	of
T2473	7344-7351	NN	denotes	annexin
T2474	7352-7354	NN	denotes	A7
T2475	7355-7358	CC	denotes	and
T2476	7359-7362	PRP$	denotes	its
T2477	7363-7370	NN	denotes	partner
T2478	7371-7377	NN	denotes	sorcin
T2479	7378-7380	IN	denotes	in
T2480	7381-7386	NN	denotes	micro
T2481	7386-7387	HYPH	denotes	-
T2482	7388-7391	CC	denotes	and
T2483	7392-7404	NNS	denotes	nanovesicles
T2484	7405-7412	VBN	denotes	derived
T2485	7413-7417	IN	denotes	from
T2486	7418-7421	JJ	denotes	red
T2488	7422-7427	NN	denotes	blood
T2487	7428-7433	NNS	denotes	cells
T2489	7434-7439	WRB	denotes	where
T2491	7440-7444	PRP	denotes	they
T2492	7445-7448	VBP	denotes	are
T2490	7449-7456	VBN	denotes	located
T2493	7457-7459	IN	denotes	in
T2494	7460-7463	DT	denotes	the
T2495	7464-7469	NN	denotes	lumen
T2496	7470-7473	CC	denotes	and
T2497	7474-7477	VBP	denotes	are
T2499	7478-7482	RB	denotes	also
T2498	7483-7491	VBN	denotes	enriched
T2500	7492-7494	IN	denotes	in
T2501	7495-7503	NN	denotes	membrane
T2502	7504-7509	NNS	denotes	rafts
T2503	7510-7511	-LRB-	denotes	[
T2504	7511-7512	CD	denotes	7
T2505	7512-7513	-RRB-	denotes	]
T2506	7513-7514	.	denotes	.
T2507	7514-7608	sentence	denotes	We have now extended these findings and show here that the 51 kDa isoform is present as well.
T2508	7515-7517	PRP	denotes	We
T2510	7518-7522	VBP	denotes	have
T2511	7523-7526	RB	denotes	now
T2509	7527-7535	VBN	denotes	extended
T2512	7536-7541	DT	denotes	these
T2513	7542-7550	NNS	denotes	findings
T2514	7551-7554	CC	denotes	and
T2515	7555-7559	VBP	denotes	show
T2516	7560-7564	RB	denotes	here
T2517	7565-7569	IN	denotes	that
T2519	7570-7573	DT	denotes	the
T2521	7574-7576	CD	denotes	51
T2522	7577-7580	NN	denotes	kDa
T2520	7581-7588	NN	denotes	isoform
T2518	7589-7591	VBZ	denotes	is
T2523	7592-7599	JJ	denotes	present
T2524	7600-7602	RB	denotes	as
T2525	7603-7607	RB	denotes	well
T2526	7607-7608	.	denotes	.
T2527	7608-7719	sentence	denotes	In western blots of human red blood cells the 47 and 51 kDa isoforms were detected using mAb 203–217 (Fig. 1).
T2528	7609-7611	IN	denotes	In
T2530	7612-7619	JJ	denotes	western
T2531	7620-7625	NNS	denotes	blots
T2532	7626-7628	IN	denotes	of
T2533	7629-7634	JJ	denotes	human
T2535	7635-7638	JJ	denotes	red
T2536	7639-7644	NN	denotes	blood
T2534	7645-7650	NNS	denotes	cells
T2537	7651-7654	DT	denotes	the
T2539	7655-7657	CD	denotes	47
T2541	7658-7661	CC	denotes	and
T2542	7662-7664	CD	denotes	51
T2540	7665-7668	NN	denotes	kDa
T2538	7669-7677	NNS	denotes	isoforms
T2543	7678-7682	VBD	denotes	were
T2529	7683-7691	VBN	denotes	detected
T2544	7692-7697	VBG	denotes	using
T2545	7698-7701	NN	denotes	mAb
T2546	7702-7709	CD	denotes	203–217
T2547	7710-7711	-LRB-	denotes	(
T2548	7711-7715	NN	denotes	Fig.
T2549	7716-7717	CD	denotes	1
T2550	7717-7718	-RRB-	denotes	)
T2551	7718-7719	.	denotes	.
T2552	7719-7866	sentence	denotes	The 47 kDa isoform was detected in silver stained gels and its identity with annexin A7 confirmed by peptide mass fingerprinting (data not shown).
T2553	7720-7723	DT	denotes	The
T2555	7724-7726	CD	denotes	47
T2556	7727-7730	NN	denotes	kDa
T2554	7731-7738	NN	denotes	isoform
T2558	7739-7742	VBD	denotes	was
T2557	7743-7751	VBN	denotes	detected
T2559	7752-7754	IN	denotes	in
T2560	7755-7761	NN	denotes	silver
T2561	7762-7769	VBN	denotes	stained
T2562	7770-7774	NNS	denotes	gels
T2563	7775-7778	CC	denotes	and
T2564	7779-7782	PRP$	denotes	its
T2565	7783-7791	NN	denotes	identity
T2567	7792-7796	IN	denotes	with
T2568	7797-7804	NN	denotes	annexin
T2569	7805-7807	NN	denotes	A7
T2566	7808-7817	VBN	denotes	confirmed
T2570	7818-7820	IN	denotes	by
T2571	7821-7828	NN	denotes	peptide
T2573	7829-7833	NN	denotes	mass
T2572	7834-7848	NN	denotes	fingerprinting
T2574	7849-7850	-LRB-	denotes	(
T2576	7850-7854	NNS	denotes	data
T2577	7855-7858	RB	denotes	not
T2575	7859-7864	VBN	denotes	shown
T2578	7864-7865	-RRB-	denotes	)
T2579	7865-7866	.	denotes	.
T2580	7866-7921	sentence	denotes	The 51 kDa isoform was only detected in western blots.
T2581	7867-7870	DT	denotes	The
T2583	7871-7873	CD	denotes	51
T2584	7874-7877	NN	denotes	kDa
T2582	7878-7885	NN	denotes	isoform
T2586	7886-7889	VBD	denotes	was
T2587	7890-7894	RB	denotes	only
T2585	7895-7903	VBN	denotes	detected
T2588	7904-7906	IN	denotes	in
T2589	7907-7914	JJ	denotes	western
T2590	7915-7920	NNS	denotes	blots
T2591	7920-7921	.	denotes	.
T2592	7921-7922	sentence	denotes
T9089	7932-7942	NN	denotes	Expression
T9090	7943-7945	IN	denotes	of
T9091	7946-7953	NN	denotes	annexin
T9092	7954-7956	NN	denotes	A7
T9093	7957-7959	IN	denotes	in
T9094	7960-7965	JJ	denotes	human
T9096	7966-7969	JJ	denotes	red
T9097	7970-7975	NN	denotes	blood
T9095	7976-7981	NNS	denotes	cells
T9098	7982-7983	-LRB-	denotes	(
T9099	7983-7984	CD	denotes	1
T9100	7984-7985	-RRB-	denotes	)
T9101	7986-7989	CC	denotes	and
T9102	7990-7995	JJ	denotes	human
T9103	7996-8005	NNS	denotes	platelets
T9104	8006-8007	-LRB-	denotes	(
T9105	8007-8008	CD	denotes	2
T9106	8008-8009	-RRB-	denotes	)
T9107	8009-8010	.	denotes	.
T9108	8010-8076	sentence	denotes	Protein homogenates were separated by SDS-PAGE (12 % acrylamide).
T9109	8011-8018	NN	denotes	Protein
T9110	8019-8030	NNS	denotes	homogenates
T9112	8031-8035	VBD	denotes	were
T9111	8036-8045	VBN	denotes	separated
T9113	8046-8048	IN	denotes	by
T9114	8049-8052	NN	denotes	SDS
T9116	8052-8053	HYPH	denotes	-
T9115	8053-8057	NN	denotes	PAGE
T9117	8058-8059	-LRB-	denotes	(
T9119	8059-8061	CD	denotes	12
T9120	8062-8063	NN	denotes	%
T9118	8064-8074	NN	denotes	acrylamide
T9121	8074-8075	-RRB-	denotes	)
T9122	8075-8076	.	denotes	.
T9123	8076-8210	sentence	denotes	The resulting western blot was probed with mAb 203–217, visualization was by a secondary peroxidase coupled antibody followed by ECL.
T9124	8077-8080	DT	denotes	The
T9126	8081-8090	VBG	denotes	resulting
T9127	8091-8098	JJ	denotes	western
T9125	8099-8103	NN	denotes	blot
T9129	8104-8107	VBD	denotes	was
T9128	8108-8114	VBN	denotes	probed
T9131	8115-8119	IN	denotes	with
T9132	8120-8123	NN	denotes	mAb
T9133	8124-8131	CD	denotes	203–217
T9134	8131-8133	,	denotes	,
T9135	8133-8146	NN	denotes	visualization
T9130	8147-8150	VBD	denotes	was
T9136	8151-8153	IN	denotes	by
T9137	8154-8155	DT	denotes	a
T9139	8156-8165	JJ	denotes	secondary
T9140	8166-8176	NN	denotes	peroxidase
T9141	8177-8184	VBN	denotes	coupled
T9138	8185-8193	NN	denotes	antibody
T9142	8194-8202	VBN	denotes	followed
T9143	8203-8205	IN	denotes	by
T9144	8206-8209	NN	denotes	ECL
T9145	8209-8210	.	denotes	.
T9146	8210-8305	sentence	denotes	Both isoforms are detected in red blood cells, in platelets only the small isoform is present.
T9147	8211-8215	DT	denotes	Both
T9148	8216-8224	NNS	denotes	isoforms
T9150	8225-8228	VBP	denotes	are
T9149	8229-8237	VBN	denotes	detected
T9152	8238-8240	IN	denotes	in
T9153	8241-8244	JJ	denotes	red
T9155	8245-8250	NN	denotes	blood
T9154	8251-8256	NNS	denotes	cells
T9156	8256-8258	,	denotes	,
T9157	8258-8260	IN	denotes	in
T9158	8261-8270	NNS	denotes	platelets
T9159	8271-8275	RB	denotes	only
T9161	8276-8279	DT	denotes	the
T9162	8280-8285	JJ	denotes	small
T9160	8286-8293	NN	denotes	isoform
T9151	8294-8296	VBZ	denotes	is
T9163	8297-8304	JJ	denotes	present
T9164	8304-8305	.	denotes	.
T2594	8306-8309	PRP$	denotes	Its
T2593	8306-8463	sentence	denotes	Its presence in vesicles led to the suggestion, that, along with raft domains, annexin A7 plays a role in membrane organization and the vesiculation process.
T2595	8310-8318	NN	denotes	presence
T2597	8319-8321	IN	denotes	in
T2598	8322-8330	NNS	denotes	vesicles
T2596	8331-8334	VBD	denotes	led
T2599	8335-8337	IN	denotes	to
T2600	8338-8341	DT	denotes	the
T2601	8342-8352	NN	denotes	suggestion
T2602	8352-8354	,	denotes	,
T2603	8354-8358	IN	denotes	that
T2605	8358-8360	,	denotes	,
T2606	8360-8365	IN	denotes	along
T2607	8366-8370	IN	denotes	with
T2608	8371-8375	NN	denotes	raft
T2609	8376-8383	NNS	denotes	domains
T2610	8383-8385	,	denotes	,
T2611	8385-8392	NN	denotes	annexin
T2612	8393-8395	NN	denotes	A7
T2604	8396-8401	VBZ	denotes	plays
T2613	8402-8403	DT	denotes	a
T2614	8404-8408	NN	denotes	role
T2615	8409-8411	IN	denotes	in
T2616	8412-8420	NN	denotes	membrane
T2617	8421-8433	NN	denotes	organization
T2618	8434-8437	CC	denotes	and
T2619	8438-8441	DT	denotes	the
T2621	8442-8454	NN	denotes	vesiculation
T2620	8455-8462	NN	denotes	process
T2622	8462-8463	.	denotes	.
T2623	8463-8598	sentence	denotes	To examine this, we analysed the ability of red blood cells derived from the annexin A7 knock out mice (anxA7-/-) to form exovesicles.
T2624	8464-8466	TO	denotes	To
T2625	8467-8474	VB	denotes	examine
T2627	8475-8479	DT	denotes	this
T2628	8479-8481	,	denotes	,
T2629	8481-8483	PRP	denotes	we
T2626	8484-8492	VBD	denotes	analysed
T2630	8493-8496	DT	denotes	the
T2631	8497-8504	NN	denotes	ability
T2632	8505-8507	IN	denotes	of
T2633	8508-8511	JJ	denotes	red
T2635	8512-8517	NN	denotes	blood
T2634	8518-8523	NNS	denotes	cells
T2636	8524-8531	VBN	denotes	derived
T2637	8532-8536	IN	denotes	from
T2638	8537-8540	DT	denotes	the
T2640	8541-8548	NN	denotes	annexin
T2641	8549-8551	NN	denotes	A7
T2642	8552-8557	NN	denotes	knock
T2643	8558-8561	NN	denotes	out
T2639	8562-8566	NNS	denotes	mice
T2644	8567-8568	-LRB-	denotes	(
T2645	8568-8573	NN	denotes	anxA7
T2646	8573-8574	SYM	denotes	-
T2647	8574-8575	HYPH	denotes	/
T2648	8575-8576	SYM	denotes	-
T2649	8576-8577	-RRB-	denotes	)
T2650	8578-8580	TO	denotes	to
T2651	8581-8585	VB	denotes	form
T2652	8586-8597	NNS	denotes	exovesicles
T2653	8597-8598	.	denotes	.
T2654	8598-8734	sentence	denotes	Because of better accessibility and larger available amounts that led to clear results, we included our data obtained with human blood.
T2655	8599-8606	IN	denotes	Because
T2657	8607-8609	IN	denotes	of
T2658	8610-8616	JJR	denotes	better
T2659	8617-8630	NN	denotes	accessibility
T2660	8631-8634	CC	denotes	and
T2661	8635-8641	JJR	denotes	larger
T2663	8642-8651	JJ	denotes	available
T2662	8652-8659	NNS	denotes	amounts
T2664	8660-8664	WDT	denotes	that
T2665	8665-8668	VBD	denotes	led
T2666	8669-8671	IN	denotes	to
T2667	8672-8677	JJ	denotes	clear
T2668	8678-8685	NNS	denotes	results
T2669	8685-8687	,	denotes	,
T2670	8687-8689	PRP	denotes	we
T2656	8690-8698	VBD	denotes	included
T2671	8699-8702	PRP$	denotes	our
T2672	8703-8707	NNS	denotes	data
T2673	8708-8716	VBN	denotes	obtained
T2674	8717-8721	IN	denotes	with
T2675	8722-8727	JJ	denotes	human
T2676	8728-8733	NN	denotes	blood
T2677	8733-8734	.	denotes	.
T2678	8734-8951	sentence	denotes	Independent of the presence of annexin A7 both types of exovesicles, micro- and nanovesicles, were released after Ca2+/ionophore treatment as determined by acetylcholine esterase activity and microscopic examination.
T2679	8735-8746	JJ	denotes	Independent
T2681	8747-8749	IN	denotes	of
T2682	8750-8753	DT	denotes	the
T2683	8754-8762	NN	denotes	presence
T2684	8763-8765	IN	denotes	of
T2685	8766-8773	NN	denotes	annexin
T2686	8774-8776	NN	denotes	A7
T2687	8777-8781	DT	denotes	both
T2688	8782-8787	NNS	denotes	types
T2689	8788-8790	IN	denotes	of
T2690	8791-8802	NNS	denotes	exovesicles
T2691	8802-8804	,	denotes	,
T2692	8804-8809	AFX	denotes	micro
T2694	8809-8810	HYPH	denotes	-
T2695	8811-8814	CC	denotes	and
T2693	8815-8827	NNS	denotes	nanovesicles
T2696	8827-8829	,	denotes	,
T2697	8829-8833	VBD	denotes	were
T2680	8834-8842	VBN	denotes	released
T2698	8843-8848	IN	denotes	after
T2699	8849-8853	NN	denotes	Ca2+
T2701	8853-8854	HYPH	denotes	/
T2700	8854-8863	NN	denotes	ionophore
T2702	8864-8873	NN	denotes	treatment
T2703	8874-8876	IN	denotes	as
T2704	8877-8887	VBN	denotes	determined
T2705	8888-8890	IN	denotes	by
T2706	8891-8904	NN	denotes	acetylcholine
T2707	8905-8913	NN	denotes	esterase
T2708	8914-8922	NN	denotes	activity
T2709	8923-8926	CC	denotes	and
T2710	8927-8938	JJ	denotes	microscopic
T2711	8939-8950	NN	denotes	examination
T2712	8950-8951	.	denotes	.
T2713	8951-9047	sentence	denotes	Annexin A7 is present in both vesicle types where it is more enriched in nanovesicles (Fig. 2).
T2714	8952-8959	NN	denotes	Annexin
T2715	8960-8962	NN	denotes	A7
T2716	8963-8965	VBZ	denotes	is
T2717	8966-8973	JJ	denotes	present
T2718	8974-8976	IN	denotes	in
T2719	8977-8981	CC	denotes	both
T2721	8982-8989	NN	denotes	vesicle
T2720	8990-8995	NNS	denotes	types
T2722	8996-9001	WRB	denotes	where
T2724	9002-9004	PRP	denotes	it
T2723	9005-9007	VBZ	denotes	is
T2725	9008-9012	RBR	denotes	more
T2726	9013-9021	JJ	denotes	enriched
T2727	9022-9024	IN	denotes	in
T2728	9025-9037	NNS	denotes	nanovesicles
T2729	9038-9039	-LRB-	denotes	(
T2730	9039-9043	NN	denotes	Fig.
T2731	9044-9045	CD	denotes	2
T2732	9045-9046	-RRB-	denotes	)
T2733	9046-9047	.	denotes	.
T2734	9047-9102	sentence	denotes	The 51 kDa isoform is only detectable in nanovesicles.
T2735	9048-9051	DT	denotes	The
T2737	9052-9054	CD	denotes	51
T2738	9055-9058	NN	denotes	kDa
T2736	9059-9066	NN	denotes	isoform
T2739	9067-9069	VBZ	denotes	is
T2740	9070-9074	RB	denotes	only
T2741	9075-9085	JJ	denotes	detectable
T2742	9086-9088	IN	denotes	in
T2743	9089-9101	NNS	denotes	nanovesicles
T2744	9101-9102	.	denotes	.
T2745	9102-9316	sentence	denotes	The quantity of both vesicle types did not differ between wild type and knock out red blood cell vesicles as determined by the AChE-values (A405 nanovesicles: ~0.18; A405 microvesicles: ~0.9; both, for wt and ko).
T2746	9103-9106	DT	denotes	The
T2747	9107-9115	NN	denotes	quantity
T2749	9116-9118	IN	denotes	of
T2750	9119-9123	DT	denotes	both
T2752	9124-9131	NN	denotes	vesicle
T2751	9132-9137	NNS	denotes	types
T2753	9138-9141	VBD	denotes	did
T2754	9142-9145	RB	denotes	not
T2748	9146-9152	VB	denotes	differ
T2755	9153-9160	IN	denotes	between
T2756	9161-9165	JJ	denotes	wild
T2757	9166-9170	NN	denotes	type
T2759	9171-9174	CC	denotes	and
T2760	9175-9180	NN	denotes	knock
T2761	9181-9184	NN	denotes	out
T2762	9185-9188	JJ	denotes	red
T2764	9189-9194	NN	denotes	blood
T2763	9195-9199	NN	denotes	cell
T2758	9200-9208	NNS	denotes	vesicles
T2765	9209-9211	IN	denotes	as
T2766	9212-9222	VBN	denotes	determined
T2767	9223-9225	IN	denotes	by
T2768	9226-9229	DT	denotes	the
T2770	9230-9234	NN	denotes	AChE
T2771	9234-9235	HYPH	denotes	-
T2769	9235-9241	NNS	denotes	values
T2772	9242-9243	-LRB-	denotes	(
T2774	9243-9247	NN	denotes	A405
T2775	9248-9260	NNS	denotes	nanovesicles
T2777	9260-9262	:	denotes	:
T2778	9262-9263	SYM	denotes	~
T2776	9263-9267	CD	denotes	0.18
T2779	9267-9268	:	denotes	;
T2780	9269-9273	NN	denotes	A405
T2781	9274-9287	NNS	denotes	microvesicles
T2783	9287-9289	:	denotes	:
T2784	9289-9290	SYM	denotes	~
T2782	9290-9293	CD	denotes	0.9
T2785	9293-9294	:	denotes	;
T2773	9295-9299	DT	denotes	both
T2786	9299-9301	,	denotes	,
T2787	9301-9304	IN	denotes	for
T2788	9305-9307	NN	denotes	wt
T2789	9308-9311	CC	denotes	and
T2790	9312-9314	NN	denotes	ko
T2791	9314-9315	-RRB-	denotes	)
T2792	9315-9316	.	denotes	.
T2793	9316-9480	sentence	denotes	It appears that annexin A7, although it has been described to fuse membranes, is not a key component in the process of the formation of red blood cell exovesicles.
T2794	9317-9319	PRP	denotes	It
T2795	9320-9327	VBZ	denotes	appears
T2796	9328-9332	IN	denotes	that
T2798	9333-9340	NN	denotes	annexin
T2799	9341-9343	NN	denotes	A7
T2800	9343-9345	,	denotes	,
T2801	9345-9353	IN	denotes	although
T2803	9354-9356	PRP	denotes	it
T2804	9357-9360	VBZ	denotes	has
T2805	9361-9365	VBN	denotes	been
T2802	9366-9375	VBN	denotes	described
T2806	9376-9378	TO	denotes	to
T2807	9379-9383	VB	denotes	fuse
T2808	9384-9393	NNS	denotes	membranes
T2809	9393-9395	,	denotes	,
T2797	9395-9397	VBZ	denotes	is
T2810	9398-9401	RB	denotes	not
T2811	9402-9403	DT	denotes	a
T2813	9404-9407	JJ	denotes	key
T2812	9408-9417	NN	denotes	component
T2814	9418-9420	IN	denotes	in
T2815	9421-9424	DT	denotes	the
T2816	9425-9432	NN	denotes	process
T2817	9433-9435	IN	denotes	of
T2818	9436-9439	DT	denotes	the
T2819	9440-9449	NN	denotes	formation
T2820	9450-9452	IN	denotes	of
T2821	9453-9456	JJ	denotes	red
T2823	9457-9462	NN	denotes	blood
T2822	9463-9467	NN	denotes	cell
T2824	9468-9479	NNS	denotes	exovesicles
T2825	9479-9480	.	denotes	.
T2826	9480-9637	sentence	denotes	When we probed for the presence of sorcin in wild type and mutant vesicles we found that the sorcin levels were reduced in the mutant nanovesicles (Fig. 2).
T2827	9481-9485	WRB	denotes	When
T2829	9486-9488	PRP	denotes	we
T2828	9489-9495	VBD	denotes	probed
T2831	9496-9499	IN	denotes	for
T2832	9500-9503	DT	denotes	the
T2833	9504-9512	NN	denotes	presence
T2834	9513-9515	IN	denotes	of
T2835	9516-9522	NN	denotes	sorcin
T2836	9523-9525	IN	denotes	in
T2837	9526-9530	JJ	denotes	wild
T2838	9531-9535	NN	denotes	type
T2840	9536-9539	CC	denotes	and
T2841	9540-9546	JJ	denotes	mutant
T2839	9547-9555	NNS	denotes	vesicles
T2842	9556-9558	PRP	denotes	we
T2830	9559-9564	VBD	denotes	found
T2843	9565-9569	IN	denotes	that
T2845	9570-9573	DT	denotes	the
T2847	9574-9580	NN	denotes	sorcin
T2846	9581-9587	NNS	denotes	levels
T2848	9588-9592	VBD	denotes	were
T2844	9593-9600	VBN	denotes	reduced
T2849	9601-9603	IN	denotes	in
T2850	9604-9607	DT	denotes	the
T2852	9608-9614	JJ	denotes	mutant
T2851	9615-9627	NNS	denotes	nanovesicles
T2853	9628-9629	-LRB-	denotes	(
T2854	9629-9633	NN	denotes	Fig.
T2855	9634-9635	CD	denotes	2
T2856	9635-9636	-RRB-	denotes	)
T2857	9636-9637	.	denotes	.
T2858	9637-10382	sentence	denotes	Figure 2 Enrichment of annexin A7 and sorcin in exovesicles derived from red blood cells. Vesicles from wild type (wt) and anxA7-/- mutant (ko) were generated with Ca2+/ionophore treatment, isolated by differential centrifugation and analysed by immunoblotting with mAb 203–217 and a sorcin polyclonal antibody. In general, annexin A7 and sorcin are more abundant in nanovesicles. The 51 kDa isoform of annexin A7 (arrows) is only observed in nanovesicles. Samples of both vesicles types were normalized according to their acetylcholine esterase activity. Human red blood cells (co) were used for control and normalized independently. To study the distribution of annexin A7 in red blood cells we used self forming iodixanol density gradients.
T9240	9648-9658	NN	denotes	Enrichment
T9241	9659-9661	IN	denotes	of
T9242	9662-9669	NN	denotes	annexin
T9243	9670-9672	NN	denotes	A7
T9244	9673-9676	CC	denotes	and
T9245	9677-9683	NN	denotes	sorcin
T9246	9684-9686	IN	denotes	in
T9247	9687-9698	NNS	denotes	exovesicles
T9248	9699-9706	VBN	denotes	derived
T9249	9707-9711	IN	denotes	from
T9250	9712-9715	JJ	denotes	red
T9252	9716-9721	NN	denotes	blood
T9251	9722-9727	NNS	denotes	cells
T9253	9727-9728	.	denotes	.
T9254	9728-9950	sentence	denotes	Vesicles from wild type (wt) and anxA7-/- mutant (ko) were generated with Ca2+/ionophore treatment, isolated by differential centrifugation and analysed by immunoblotting with mAb 203–217 and a sorcin polyclonal antibody.
T9255	9729-9737	NNS	denotes	Vesicles
T9257	9738-9742	IN	denotes	from
T9258	9743-9747	JJ	denotes	wild
T9259	9748-9752	NN	denotes	type
T9260	9753-9754	-LRB-	denotes	(
T9261	9754-9756	NN	denotes	wt
T9262	9756-9757	-RRB-	denotes	)
T9263	9758-9761	CC	denotes	and
T9264	9762-9767	JJ	denotes	anxA7
T9266	9767-9768	SYM	denotes	-
T9267	9768-9769	HYPH	denotes	/
T9268	9769-9770	SYM	denotes	-
T9265	9771-9777	NN	denotes	mutant
T9269	9778-9779	-LRB-	denotes	(
T9270	9779-9781	NN	denotes	ko
T9271	9781-9782	-RRB-	denotes	)
T9272	9783-9787	VBD	denotes	were
T9256	9788-9797	VBN	denotes	generated
T9273	9798-9802	IN	denotes	with
T9274	9803-9807	NN	denotes	Ca2+
T9276	9807-9808	HYPH	denotes	/
T9275	9808-9817	NN	denotes	ionophore
T9277	9818-9827	NN	denotes	treatment
T9278	9827-9829	,	denotes	,
T9279	9829-9837	VBN	denotes	isolated
T9280	9838-9840	IN	denotes	by
T9281	9841-9853	JJ	denotes	differential
T9282	9854-9868	NN	denotes	centrifugation
T9283	9869-9872	CC	denotes	and
T9284	9873-9881	VBN	denotes	analysed
T9285	9882-9884	IN	denotes	by
T9286	9885-9899	VBG	denotes	immunoblotting
T9287	9900-9904	IN	denotes	with
T9288	9905-9908	NN	denotes	mAb
T9289	9909-9912	CD	denotes	203
T9290	9912-9913	SYM	denotes	–
T9291	9913-9916	CD	denotes	217
T9292	9917-9920	CC	denotes	and
T9293	9921-9922	DT	denotes	a
T9295	9923-9929	NN	denotes	sorcin
T9296	9930-9940	JJ	denotes	polyclonal
T9294	9941-9949	NN	denotes	antibody
T9297	9949-9950	.	denotes	.
T9298	9950-10019	sentence	denotes	In general, annexin A7 and sorcin are more abundant in nanovesicles.
T9299	9951-9953	IN	denotes	In
T9301	9954-9961	JJ	denotes	general
T9302	9961-9963	,	denotes	,
T9303	9963-9970	NN	denotes	annexin
T9304	9971-9973	NN	denotes	A7
T9305	9974-9977	CC	denotes	and
T9306	9978-9984	NN	denotes	sorcin
T9300	9985-9988	VBP	denotes	are
T9307	9989-9993	RBR	denotes	more
T9308	9994-10002	JJ	denotes	abundant
T9309	10003-10005	IN	denotes	in
T9310	10006-10018	NNS	denotes	nanovesicles
T9311	10018-10019	.	denotes	.
T9312	10019-10095	sentence	denotes	The 51 kDa isoform of annexin A7 (arrows) is only observed in nanovesicles.
T9313	10020-10023	DT	denotes	The
T9315	10024-10026	CD	denotes	51
T9316	10027-10030	NN	denotes	kDa
T9314	10031-10038	NN	denotes	isoform
T9318	10039-10041	IN	denotes	of
T9319	10042-10049	NN	denotes	annexin
T9320	10050-10052	NN	denotes	A7
T9321	10053-10054	-LRB-	denotes	(
T9322	10054-10060	NNS	denotes	arrows
T9323	10060-10061	-RRB-	denotes	)
T9324	10062-10064	VBZ	denotes	is
T9325	10065-10069	RB	denotes	only
T9317	10070-10078	VBN	denotes	observed
T9326	10079-10081	IN	denotes	in
T9327	10082-10094	NNS	denotes	nanovesicles
T9328	10094-10095	.	denotes	.
T9329	10095-10194	sentence	denotes	Samples of both vesicles types were normalized according to their acetylcholine esterase activity.
T9330	10096-10103	NNS	denotes	Samples
T9332	10104-10106	IN	denotes	of
T9333	10107-10111	DT	denotes	both
T9335	10112-10120	NNS	denotes	vesicles
T9334	10121-10126	NNS	denotes	types
T9336	10127-10131	VBD	denotes	were
T9331	10132-10142	VBN	denotes	normalized
T9337	10143-10152	VBG	denotes	according
T9338	10153-10155	IN	denotes	to
T9339	10156-10161	PRP$	denotes	their
T9341	10162-10175	NN	denotes	acetylcholine
T9342	10176-10184	NN	denotes	esterase
T9340	10185-10193	NN	denotes	activity
T9343	10193-10194	.	denotes	.
T9344	10194-10273	sentence	denotes	Human red blood cells (co) were used for control and normalized independently.
T9345	10195-10200	JJ	denotes	Human
T9347	10201-10204	JJ	denotes	red
T9348	10205-10210	NN	denotes	blood
T9346	10211-10216	NNS	denotes	cells
T9350	10217-10218	-LRB-	denotes	(
T9351	10218-10220	NN	denotes	co
T9352	10220-10221	-RRB-	denotes	)
T9353	10222-10226	VBD	denotes	were
T9349	10227-10231	VBN	denotes	used
T9354	10232-10235	IN	denotes	for
T9355	10236-10243	NN	denotes	control
T9356	10244-10247	CC	denotes	and
T9357	10248-10258	VBD	denotes	normalized
T9358	10259-10272	RB	denotes	independently
T9359	10272-10273	.	denotes	.
T2859	10274-10276	TO	denotes	To
T2860	10277-10282	VB	denotes	study
T2862	10283-10286	DT	denotes	the
T2863	10287-10299	NN	denotes	distribution
T2864	10300-10302	IN	denotes	of
T2865	10303-10310	NN	denotes	annexin
T2866	10311-10313	NN	denotes	A7
T2867	10314-10316	IN	denotes	in
T2868	10317-10320	JJ	denotes	red
T2870	10321-10326	NN	denotes	blood
T2869	10327-10332	NNS	denotes	cells
T2871	10333-10335	PRP	denotes	we
T2861	10336-10340	VBD	denotes	used
T2872	10341-10345	NN	denotes	self
T2873	10346-10353	VBG	denotes	forming
T2875	10354-10363	NN	denotes	iodixanol
T2876	10364-10371	NN	denotes	density
T2874	10372-10381	NNS	denotes	gradients
T2877	10381-10382	.	denotes	.
T2878	10382-10526	sentence	denotes	Both isoforms were present in the soluble fraction as well as in the gradient fractions where they are assumed to be associated with membranes.
T2879	10383-10387	DT	denotes	Both
T2880	10388-10396	NNS	denotes	isoforms
T2881	10397-10401	VBD	denotes	were
T2882	10402-10409	JJ	denotes	present
T2883	10410-10412	IN	denotes	in
T2884	10413-10416	DT	denotes	the
T2886	10417-10424	JJ	denotes	soluble
T2885	10425-10433	NN	denotes	fraction
T2887	10434-10436	RB	denotes	as
T2889	10437-10441	RB	denotes	well
T2888	10442-10444	IN	denotes	as
T2890	10445-10447	IN	denotes	in
T2891	10448-10451	DT	denotes	the
T2893	10452-10460	NN	denotes	gradient
T2892	10461-10470	NNS	denotes	fractions
T2894	10471-10476	WRB	denotes	where
T2896	10477-10481	PRP	denotes	they
T2897	10482-10485	VBP	denotes	are
T2895	10486-10493	VBN	denotes	assumed
T2898	10494-10496	TO	denotes	to
T2900	10497-10499	VB	denotes	be
T2899	10500-10510	VBN	denotes	associated
T2901	10511-10515	IN	denotes	with
T2902	10516-10525	NNS	denotes	membranes
T2903	10525-10526	.	denotes	.
T2904	10526-10622	sentence	denotes	These membranes are exclusively plasma membranes as red blood cells are free of any organelles.
T2905	10527-10532	DT	denotes	These
T2906	10533-10542	NNS	denotes	membranes
T2907	10543-10546	VBP	denotes	are
T2908	10547-10558	RB	denotes	exclusively
T2909	10559-10565	NN	denotes	plasma
T2910	10566-10575	NNS	denotes	membranes
T2911	10576-10578	IN	denotes	as
T2913	10579-10582	JJ	denotes	red
T2915	10583-10588	NN	denotes	blood
T2914	10589-10594	NNS	denotes	cells
T2912	10595-10598	VBP	denotes	are
T2916	10599-10603	JJ	denotes	free
T2917	10604-10606	IN	denotes	of
T2918	10607-10610	DT	denotes	any
T2919	10611-10621	NNS	denotes	organelles
T2920	10621-10622	.	denotes	.
T2921	10622-10692	sentence	denotes	The distribution was however not homogeneous throughout the gradient.
T2922	10623-10626	DT	denotes	The
T2923	10627-10639	NN	denotes	distribution
T2924	10640-10643	VBD	denotes	was
T2925	10644-10651	RB	denotes	however
T2926	10652-10655	RB	denotes	not
T2927	10656-10667	JJ	denotes	homogeneous
T2928	10668-10678	IN	denotes	throughout
T2929	10679-10682	DT	denotes	the
T2930	10683-10691	NN	denotes	gradient
T2931	10691-10692	.	denotes	.
T2932	10692-10850	sentence	denotes	This could reflect the binding of annexin A7 to membrane subdomains that have different lipid or lipid/protein composition as discussed by Salzer et al. [7].
T2933	10693-10697	DT	denotes	This
T2935	10698-10703	MD	denotes	could
T2934	10704-10711	VB	denotes	reflect
T2936	10712-10715	DT	denotes	the
T2937	10716-10723	NN	denotes	binding
T2938	10724-10726	IN	denotes	of
T2939	10727-10734	NN	denotes	annexin
T2940	10735-10737	NN	denotes	A7
T2941	10738-10740	IN	denotes	to
T2942	10741-10749	NN	denotes	membrane
T2943	10750-10760	NNS	denotes	subdomains
T2944	10761-10765	WDT	denotes	that
T2945	10766-10770	VBP	denotes	have
T2946	10771-10780	JJ	denotes	different
T2948	10781-10786	NN	denotes	lipid
T2949	10787-10789	CC	denotes	or
T2950	10790-10795	NN	denotes	lipid
T2952	10795-10796	HYPH	denotes	/
T2951	10796-10803	NN	denotes	protein
T2947	10804-10815	NN	denotes	composition
T2953	10816-10818	IN	denotes	as
T2954	10819-10828	VBN	denotes	discussed
T2955	10829-10831	IN	denotes	by
T2956	10832-10838	NNP	denotes	Salzer
T2957	10839-10841	FW	denotes	et
T2958	10842-10845	FW	denotes	al.
T2959	10846-10847	-LRB-	denotes	[
T2960	10847-10848	CD	denotes	7
T2961	10848-10849	-RRB-	denotes	]
T2962	10849-10850	.	denotes	.
T2963	10850-10928	sentence	denotes	Likewise, sorcin does not exhibit a homogeneous distribution in the gradient.
T2964	10851-10859	RB	denotes	Likewise
T2966	10859-10861	,	denotes	,
T2967	10861-10867	NN	denotes	sorcin
T2968	10868-10872	VBZ	denotes	does
T2969	10873-10876	RB	denotes	not
T2965	10877-10884	VB	denotes	exhibit
T2970	10885-10886	DT	denotes	a
T2972	10887-10898	JJ	denotes	homogeneous
T2971	10899-10911	NN	denotes	distribution
T2973	10912-10914	IN	denotes	in
T2974	10915-10918	DT	denotes	the
T2975	10919-10927	NN	denotes	gradient
T2976	10927-10928	.	denotes	.
T2977	10928-11017	sentence	denotes	Moreover, it segregates into vesicles which are not associated with annexin A7 (Fig. 3).
T2978	10929-10937	RB	denotes	Moreover
T2980	10937-10939	,	denotes	,
T2981	10939-10941	PRP	denotes	it
T2979	10942-10952	VBZ	denotes	segregates
T2982	10953-10957	IN	denotes	into
T2983	10958-10966	NNS	denotes	vesicles
T2984	10967-10972	WDT	denotes	which
T2986	10973-10976	VBP	denotes	are
T2987	10977-10980	RB	denotes	not
T2985	10981-10991	VBN	denotes	associated
T2988	10992-10996	IN	denotes	with
T2989	10997-11004	NN	denotes	annexin
T2990	11005-11007	NN	denotes	A7
T2991	11008-11009	-LRB-	denotes	(
T2992	11009-11013	NN	denotes	Fig.
T2993	11014-11015	CD	denotes	3
T2994	11015-11016	-RRB-	denotes	)
T2995	11016-11017	.	denotes	.
T2996	11017-11110	sentence	denotes	We also tested platelets for annexin A7 expression and detected the 47 kDa isoform (Fig. 1).
T2997	11018-11020	PRP	denotes	We
T2999	11021-11025	RB	denotes	also
T2998	11026-11032	VBD	denotes	tested
T3000	11033-11042	NNS	denotes	platelets
T3001	11043-11046	IN	denotes	for
T3002	11047-11054	NN	denotes	annexin
T3003	11055-11057	NN	denotes	A7
T3004	11058-11068	NN	denotes	expression
T3005	11069-11072	CC	denotes	and
T3006	11073-11081	VBD	denotes	detected
T3007	11082-11085	DT	denotes	the
T3009	11086-11088	CD	denotes	47
T3010	11089-11092	NN	denotes	kDa
T3008	11093-11100	NN	denotes	isoform
T3011	11101-11102	-LRB-	denotes	(
T3012	11102-11106	NN	denotes	Fig.
T3013	11107-11108	CD	denotes	1
T3014	11108-11109	-RRB-	denotes	)
T3015	11109-11110	.	denotes	.
T9397	11121-11132	NN	denotes	Association
T9398	11133-11135	IN	denotes	of
T9399	11136-11143	NN	denotes	annexin
T9400	11144-11146	NN	denotes	A7
T9401	11147-11150	CC	denotes	and
T9402	11151-11157	NN	denotes	sorcin
T9403	11158-11162	IN	denotes	with
T9404	11163-11171	JJ	denotes	distinct
T9406	11172-11175	JJ	denotes	red
T9408	11176-11181	NN	denotes	blood
T9407	11182-11186	NN	denotes	cell
T9409	11187-11193	NN	denotes	plasma
T9410	11194-11202	NN	denotes	membrane
T9405	11203-11212	NNS	denotes	fractions
T9411	11212-11213	.	denotes	.
T9412	11213-11298	sentence	denotes	Lysed human red blood cells were added to a self forming iodixanol density gradient.
T9413	11214-11219	VBN	denotes	Lysed
T9415	11220-11225	JJ	denotes	human
T9416	11226-11229	JJ	denotes	red
T9417	11230-11235	NN	denotes	blood
T9414	11236-11241	NNS	denotes	cells
T9419	11242-11246	VBD	denotes	were
T9418	11247-11252	VBN	denotes	added
T9420	11253-11255	IN	denotes	to
T9421	11256-11257	DT	denotes	a
T9423	11258-11262	NN	denotes	self
T9424	11263-11270	VBG	denotes	forming
T9425	11271-11280	NN	denotes	iodixanol
T9426	11281-11288	NN	denotes	density
T9422	11289-11297	NN	denotes	gradient
T9427	11297-11298	.	denotes	.
T9428	11298-11380	sentence	denotes	The density of the gradient increases from left (1.06 g/ml) to right (1.20 g/ml).
T9429	11299-11302	DT	denotes	The
T9430	11303-11310	NN	denotes	density
T9432	11311-11313	IN	denotes	of
T9433	11314-11317	DT	denotes	the
T9434	11318-11326	NN	denotes	gradient
T9431	11327-11336	VBZ	denotes	increases
T9435	11337-11341	IN	denotes	from
T9436	11342-11346	JJ	denotes	left
T9437	11347-11348	-LRB-	denotes	(
T9439	11348-11352	CD	denotes	1.06
T9438	11353-11354	NNS	denotes	g
T9440	11354-11355	SYM	denotes	/
T9441	11355-11357	NN	denotes	ml
T9442	11357-11358	-RRB-	denotes	)
T9443	11359-11361	IN	denotes	to
T9444	11362-11367	JJ	denotes	right
T9445	11368-11369	-LRB-	denotes	(
T9447	11369-11373	CD	denotes	1.20
T9446	11374-11375	NNS	denotes	g
T9448	11375-11376	SYM	denotes	/
T9449	11376-11378	NN	denotes	ml
T9450	11378-11379	-RRB-	denotes	)
T9451	11379-11380	.	denotes	.
T9452	11380-11476	sentence	denotes	Fractions were analysed by immunoblotting using mAb 203–217 and the sorcin polyclonal antibody.
T9453	11381-11390	NNS	denotes	Fractions
T9455	11391-11395	VBD	denotes	were
T9454	11396-11404	VBN	denotes	analysed
T9456	11405-11407	IN	denotes	by
T9457	11408-11422	NN	denotes	immunoblotting
T9458	11423-11428	VBG	denotes	using
T9459	11429-11432	NN	denotes	mAb
T9460	11433-11436	CD	denotes	203
T9461	11436-11437	SYM	denotes	–
T9462	11437-11440	CD	denotes	217
T9463	11441-11444	CC	denotes	and
T9464	11445-11448	DT	denotes	the
T9466	11449-11455	NN	denotes	sorcin
T9467	11456-11466	JJ	denotes	polyclonal
T9465	11467-11475	NN	denotes	antibody
T9468	11475-11476	.	denotes	.
T3280	11478-11481	DT	denotes	The
T3281	11482-11486	NN	denotes	lack
T3283	11487-11489	IN	denotes	of
T3284	11490-11497	NN	denotes	annexin
T3285	11498-11500	NN	denotes	A7
T3282	11501-11508	VBZ	denotes	changes
T3286	11509-11512	JJ	denotes	red
T3288	11513-11518	NN	denotes	blood
T3287	11519-11523	NN	denotes	cell
T3289	11524-11534	NN	denotes	morphology
T3290	11535-11538	CC	denotes	and
T3291	11539-11546	JJ	denotes	osmotic
T3292	11547-11557	NN	denotes	resistance
T3293	11557-11743	sentence	denotes	As annexin A7 is a component of red blood cell vesicles we studied the consequences of the loss of the protein in the anxA7-/-mouse for red blood cell morphology and osmotic resistance.
T3294	11558-11560	IN	denotes	As
T3296	11561-11568	NN	denotes	annexin
T3297	11569-11571	NN	denotes	A7
T3295	11572-11574	VBZ	denotes	is
T3299	11575-11576	DT	denotes	a
T3300	11577-11586	NN	denotes	component
T3301	11587-11589	IN	denotes	of
T3302	11590-11593	JJ	denotes	red
T3304	11594-11599	NN	denotes	blood
T3303	11600-11604	NN	denotes	cell
T3305	11605-11613	NNS	denotes	vesicles
T3306	11614-11616	PRP	denotes	we
T3298	11617-11624	VBD	denotes	studied
T3307	11625-11628	DT	denotes	the
T3308	11629-11641	NNS	denotes	consequences
T3309	11642-11644	IN	denotes	of
T3310	11645-11648	DT	denotes	the
T3311	11649-11653	NN	denotes	loss
T3312	11654-11656	IN	denotes	of
T3313	11657-11660	DT	denotes	the
T3314	11661-11668	NN	denotes	protein
T3315	11669-11671	IN	denotes	in
T3316	11672-11675	DT	denotes	the
T3318	11676-11681	NN	denotes	anxA7
T3319	11681-11682	SYM	denotes	-
T3320	11682-11683	HYPH	denotes	/
T3321	11683-11684	SYM	denotes	-
T3317	11684-11689	NN	denotes	mouse
T3322	11690-11693	IN	denotes	for
T3323	11694-11697	JJ	denotes	red
T3325	11698-11703	NN	denotes	blood
T3324	11704-11708	NN	denotes	cell
T3326	11709-11719	NN	denotes	morphology
T3327	11720-11723	CC	denotes	and
T3328	11724-11731	JJ	denotes	osmotic
T3329	11732-11742	NN	denotes	resistance
T3330	11742-11743	.	denotes	.
T3331	11743-11916	sentence	denotes	In a standard 'blood smear' we did not note a deformation of the cells, however dark field microscopy revealed changes in shape and diameter of the anxA7-/-red blood cells.
T3332	11744-11746	IN	denotes	In
T3334	11747-11748	DT	denotes	a
T3336	11749-11757	JJ	denotes	standard
T3337	11758-11759	``	denotes	'
T3338	11759-11764	NN	denotes	blood
T3335	11765-11770	NN	denotes	smear
T3339	11770-11771	''	denotes	'
T3340	11772-11774	PRP	denotes	we
T3342	11775-11778	VBD	denotes	did
T3343	11779-11782	RB	denotes	not
T3341	11783-11787	VB	denotes	note
T3344	11788-11789	DT	denotes	a
T3345	11790-11801	NN	denotes	deformation
T3346	11802-11804	IN	denotes	of
T3347	11805-11808	DT	denotes	the
T3348	11809-11814	NNS	denotes	cells
T3349	11814-11816	,	denotes	,
T3350	11816-11823	RB	denotes	however
T3351	11824-11828	JJ	denotes	dark
T3352	11829-11834	NN	denotes	field
T3353	11835-11845	NN	denotes	microscopy
T3333	11846-11854	VBD	denotes	revealed
T3354	11855-11862	NNS	denotes	changes
T3355	11863-11865	IN	denotes	in
T3356	11866-11871	NN	denotes	shape
T3357	11872-11875	CC	denotes	and
T3358	11876-11884	NN	denotes	diameter
T3359	11885-11887	IN	denotes	of
T3360	11888-11891	DT	denotes	the
T3362	11892-11897	JJ	denotes	anxA7
T3363	11897-11898	SYM	denotes	-
T3364	11898-11899	HYPH	denotes	/
T3365	11899-11900	SYM	denotes	-
T3366	11900-11903	JJ	denotes	red
T3367	11904-11909	NN	denotes	blood
T3361	11910-11915	NNS	denotes	cells
T3368	11915-11916	.	denotes	.
T3369	11916-12120	sentence	denotes	They had a statistically significant larger cell diameter of 6.0 μm compared to wild type with 5.7 μm (p = 0.015, n = 40), a remarkably lower emphasized central impression and a more flat shape (Fig. 4).
T3370	11917-11921	PRP	denotes	They
T3371	11922-11925	VBD	denotes	had
T3372	11926-11927	DT	denotes	a
T3374	11928-11941	RB	denotes	statistically
T3375	11942-11953	JJ	denotes	significant
T3376	11954-11960	JJR	denotes	larger
T3377	11961-11965	NN	denotes	cell
T3373	11966-11974	NN	denotes	diameter
T3378	11975-11977	IN	denotes	of
T3379	11978-11981	CD	denotes	6.0
T3380	11982-11984	NN	denotes	μm
T3381	11985-11993	VBN	denotes	compared
T3382	11994-11996	IN	denotes	to
T3383	11997-12001	JJ	denotes	wild
T3384	12002-12006	NN	denotes	type
T3385	12007-12011	IN	denotes	with
T3386	12012-12015	CD	denotes	5.7
T3387	12016-12018	NNS	denotes	μm
T3388	12019-12020	-LRB-	denotes	(
T3390	12020-12021	NN	denotes	p
T3392	12022-12023	SYM	denotes	=
T3391	12024-12029	CD	denotes	0.015
T3393	12029-12031	,	denotes	,
T3394	12031-12032	NN	denotes	n
T3395	12033-12034	SYM	denotes	=
T3389	12035-12037	CD	denotes	40
T3396	12037-12038	-RRB-	denotes	)
T3397	12038-12040	,	denotes	,
T3398	12040-12041	DT	denotes	a
T3400	12042-12052	RB	denotes	remarkably
T3401	12053-12058	JJR	denotes	lower
T3402	12059-12069	VBN	denotes	emphasized
T3403	12070-12077	JJ	denotes	central
T3399	12078-12088	NN	denotes	impression
T3404	12089-12092	CC	denotes	and
T3405	12093-12094	DT	denotes	a
T3407	12095-12099	RBR	denotes	more
T3408	12100-12104	JJ	denotes	flat
T3406	12105-12110	NN	denotes	shape
T3409	12111-12112	-LRB-	denotes	(
T3410	12112-12116	NN	denotes	Fig.
T3411	12117-12118	CD	denotes	4
T3412	12118-12119	-RRB-	denotes	)
T3413	12119-12120	.	denotes	.
T3414	12120-12225	sentence	denotes	No significant change in the mean corpuscular volume (MCV) was measured with the ADVIA 120 cell counter.
T3415	12121-12123	DT	denotes	No
T3417	12124-12135	JJ	denotes	significant
T3416	12136-12142	NN	denotes	change
T3419	12143-12145	IN	denotes	in
T3420	12146-12149	DT	denotes	the
T3422	12150-12154	JJ	denotes	mean
T3423	12155-12166	JJ	denotes	corpuscular
T3421	12167-12173	NN	denotes	volume
T3424	12174-12175	-LRB-	denotes	(
T3425	12175-12178	NN	denotes	MCV
T3426	12178-12179	-RRB-	denotes	)
T3427	12180-12183	VBD	denotes	was
T3418	12184-12192	VBN	denotes	measured
T3428	12193-12197	IN	denotes	with
T3429	12198-12201	DT	denotes	the
T3431	12202-12207	NN	denotes	ADVIA
T3432	12208-12211	CD	denotes	120
T3433	12212-12216	NN	denotes	cell
T3430	12217-12224	NN	denotes	counter
T3434	12224-12225	.	denotes	.
T3435	12225-12226	sentence	denotes
T9489	12236-12240	JJ	denotes	Dark
T9490	12241-12246	NN	denotes	field
T9491	12247-12257	NN	denotes	microscopy
T9492	12258-12260	IN	denotes	of
T9493	12261-12264	JJ	denotes	red
T9495	12265-12270	NN	denotes	blood
T9494	12271-12276	NNS	denotes	cells
T9496	12277-12281	IN	denotes	from
T9497	12282-12287	JJ	denotes	anxA7
T9499	12287-12288	SYM	denotes	-
T9500	12288-12289	HYPH	denotes	/
T9501	12289-12290	SYM	denotes	-
T9498	12291-12297	NN	denotes	mutant
T9503	12298-12299	-LRB-	denotes	(
T9504	12299-12300	NN	denotes	B
T9505	12300-12301	-RRB-	denotes	)
T9506	12302-12305	CC	denotes	and
T9507	12306-12310	JJ	denotes	wild
T9508	12311-12315	NN	denotes	type
T9502	12316-12320	NNS	denotes	mice
T9509	12321-12322	-LRB-	denotes	(
T9510	12322-12323	NN	denotes	A
T9511	12323-12324	-RRB-	denotes	)
T9512	12324-12325	.	denotes	.
T9513	12325-12390	sentence	denotes	The mean cellular diameters are 6.0 μm and 5.7 μm, respectively.
T9514	12326-12329	DT	denotes	The
T9516	12330-12334	JJ	denotes	mean
T9517	12335-12343	JJ	denotes	cellular
T9515	12344-12353	NNS	denotes	diameters
T9518	12354-12357	VBP	denotes	are
T9519	12358-12361	CD	denotes	6.0
T9520	12362-12364	NNS	denotes	μm
T9521	12365-12368	CC	denotes	and
T9522	12369-12372	CD	denotes	5.7
T9523	12373-12375	NNS	denotes	μm
T9524	12375-12377	,	denotes	,
T9525	12377-12389	RB	denotes	respectively
T9526	12389-12390	.	denotes	.
T9527	12390-12462	sentence	denotes	The size differences are statistically significant (p = 0.015; n = 40).
T9528	12391-12394	DT	denotes	The
T9530	12395-12399	NN	denotes	size
T9529	12400-12411	NNS	denotes	differences
T9531	12412-12415	VBP	denotes	are
T9532	12416-12429	RB	denotes	statistically
T9533	12430-12441	JJ	denotes	significant
T9534	12442-12443	-LRB-	denotes	(
T9536	12443-12444	NN	denotes	p
T9538	12445-12446	SYM	denotes	=
T9537	12447-12452	CD	denotes	0.015
T9539	12452-12453	:	denotes	;
T9540	12454-12455	NN	denotes	n
T9541	12456-12457	SYM	denotes	=
T9535	12458-12460	CD	denotes	40
T9542	12460-12461	-RRB-	denotes	)
T9543	12461-12462	.	denotes	.
T9544	12462-12473	sentence	denotes	Bar, 3 μm.
T9545	12463-12466	NN	denotes	Bar
T9547	12466-12468	,	denotes	,
T9548	12468-12469	CD	denotes	3
T9546	12470-12472	NNS	denotes	μm
T9549	12472-12473	.	denotes	.
T3437	12474-12479	NN	denotes	Shape
T3436	12474-12565	sentence	denotes	Shape and MCV of a red blood cell essentially influence its function and capillary passage.
T3439	12480-12483	CC	denotes	and
T3440	12484-12487	NN	denotes	MCV
T3441	12488-12490	IN	denotes	of
T3442	12491-12492	DT	denotes	a
T3444	12493-12496	JJ	denotes	red
T3445	12497-12502	NN	denotes	blood
T3443	12503-12507	NN	denotes	cell
T3446	12508-12519	RB	denotes	essentially
T3438	12520-12529	VB	denotes	influence
T3447	12530-12533	PRP$	denotes	its
T3448	12534-12542	NN	denotes	function
T3449	12543-12546	CC	denotes	and
T3450	12547-12556	JJ	denotes	capillary
T3451	12557-12564	NN	denotes	passage
T3452	12564-12565	.	denotes	.
T3453	12565-12739	sentence	denotes	The typical biconcave form depends on various influences including the membrane lipid composition and the submembranous cytoskeleton [32] where annexin A7 might play a role.
T3454	12566-12569	DT	denotes	The
T3456	12570-12577	JJ	denotes	typical
T3457	12578-12587	NN	denotes	biconcave
T3455	12588-12592	NN	denotes	form
T3458	12593-12600	VBZ	denotes	depends
T3459	12601-12603	IN	denotes	on
T3460	12604-12611	JJ	denotes	various
T3461	12612-12622	NNS	denotes	influences
T3462	12623-12632	VBG	denotes	including
T3463	12633-12636	DT	denotes	the
T3465	12637-12645	NN	denotes	membrane
T3466	12646-12651	NN	denotes	lipid
T3464	12652-12663	NN	denotes	composition
T3467	12664-12667	CC	denotes	and
T3468	12668-12671	DT	denotes	the
T3470	12672-12685	JJ	denotes	submembranous
T3469	12686-12698	NN	denotes	cytoskeleton
T3471	12699-12700	-LRB-	denotes	[
T3472	12700-12702	CD	denotes	32
T3473	12702-12703	-RRB-	denotes	]
T3474	12704-12709	WRB	denotes	where
T3476	12710-12717	NN	denotes	annexin
T3477	12718-12720	NN	denotes	A7
T3478	12721-12726	MD	denotes	might
T3475	12727-12731	VB	denotes	play
T3479	12732-12733	DT	denotes	a
T3480	12734-12738	NN	denotes	role
T3481	12738-12739	.	denotes	.
T3482	12739-12908	sentence	denotes	The osmotic resistance, which is the resistance towards changes in the extracellular ionic strength, is a convenient assay for analysis of the red blood cell integrity.
T3483	12740-12743	DT	denotes	The
T3485	12744-12751	JJ	denotes	osmotic
T3484	12752-12762	NN	denotes	resistance
T3487	12762-12764	,	denotes	,
T3488	12764-12769	WDT	denotes	which
T3489	12770-12772	VBZ	denotes	is
T3490	12773-12776	DT	denotes	the
T3491	12777-12787	NN	denotes	resistance
T3492	12788-12795	IN	denotes	towards
T3493	12796-12803	NNS	denotes	changes
T3494	12804-12806	IN	denotes	in
T3495	12807-12810	DT	denotes	the
T3497	12811-12824	JJ	denotes	extracellular
T3498	12825-12830	JJ	denotes	ionic
T3496	12831-12839	NN	denotes	strength
T3499	12839-12841	,	denotes	,
T3486	12841-12843	VBZ	denotes	is
T3500	12844-12845	DT	denotes	a
T3502	12846-12856	JJ	denotes	convenient
T3501	12857-12862	NN	denotes	assay
T3503	12863-12866	IN	denotes	for
T3504	12867-12875	NN	denotes	analysis
T3505	12876-12878	IN	denotes	of
T3506	12879-12882	DT	denotes	the
T3508	12883-12886	JJ	denotes	red
T3510	12887-12892	NN	denotes	blood
T3509	12893-12897	NN	denotes	cell
T3507	12898-12907	NN	denotes	integrity
T3511	12907-12908	.	denotes	.
T3512	12908-13075	sentence	denotes	It is measured as the sodium chloride concentration at which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of the cells [33].
T3513	12909-12911	PRP	denotes	It
T3515	12912-12914	VBZ	denotes	is
T3514	12915-12923	VBN	denotes	measured
T3516	12924-12926	IN	denotes	as
T3517	12927-12930	DT	denotes	the
T3519	12931-12937	NN	denotes	sodium
T3520	12938-12946	NN	denotes	chloride
T3518	12947-12960	NN	denotes	concentration
T3521	12961-12963	IN	denotes	at
T3523	12964-12969	WDT	denotes	which
T3524	12970-12978	JJ	denotes	cellular
T3525	12979-12984	NN	denotes	lysis
T3522	12985-12991	VBZ	denotes	starts
T3526	12992-12993	-LRB-	denotes	(
T3527	12993-13000	JJ	denotes	minimal
T3528	13001-13011	NN	denotes	resistance
T3529	13011-13012	-RRB-	denotes	)
T3530	13013-13015	IN	denotes	up
T3531	13016-13018	IN	denotes	to
T3532	13019-13020	DT	denotes	a
T3534	13021-13029	JJ	denotes	complete
T3533	13030-13035	NN	denotes	lysis
T3535	13036-13037	-LRB-	denotes	(
T3536	13037-13044	JJ	denotes	maximal
T3537	13045-13055	NN	denotes	resistance
T3538	13055-13056	-RRB-	denotes	)
T3539	13057-13059	IN	denotes	of
T3540	13060-13063	DT	denotes	the
T3541	13064-13069	NNS	denotes	cells
T3542	13070-13071	-LRB-	denotes	[
T3543	13071-13073	CD	denotes	33
T3544	13073-13074	-RRB-	denotes	]
T3545	13074-13075	.	denotes	.
T3546	13075-13226	sentence	denotes	Osmotic resistance increases with higher MCV, larger surface area and a higher degree of cellular metabolism stabilizing the intracellular ion levels.
T3547	13076-13083	JJ	denotes	Osmotic
T3548	13084-13094	NN	denotes	resistance
T3549	13095-13104	VBZ	denotes	increases
T3550	13105-13109	IN	denotes	with
T3551	13110-13116	JJR	denotes	higher
T3552	13117-13120	NN	denotes	MCV
T3553	13120-13122	,	denotes	,
T3554	13122-13128	JJR	denotes	larger
T3556	13129-13136	NN	denotes	surface
T3555	13137-13141	NN	denotes	area
T3557	13142-13145	CC	denotes	and
T3558	13146-13147	DT	denotes	a
T3560	13148-13154	JJR	denotes	higher
T3559	13155-13161	NN	denotes	degree
T3561	13162-13164	IN	denotes	of
T3562	13165-13173	JJ	denotes	cellular
T3563	13174-13184	NN	denotes	metabolism
T3564	13185-13196	VBG	denotes	stabilizing
T3565	13197-13200	DT	denotes	the
T3567	13201-13214	JJ	denotes	intracellular
T3568	13215-13218	NN	denotes	ion
T3566	13219-13225	NNS	denotes	levels
T3569	13225-13226	.	denotes	.
T3570	13226-13301	sentence	denotes	Aged red blood cells show a lowered MCV and lower osmotic resistance [33].
T3571	13227-13231	VBN	denotes	Aged
T3573	13232-13235	JJ	denotes	red
T3574	13236-13241	NN	denotes	blood
T3572	13242-13247	NNS	denotes	cells
T3575	13248-13252	VBP	denotes	show
T3576	13253-13254	DT	denotes	a
T3578	13255-13262	JJ	denotes	lowered
T3577	13263-13266	NN	denotes	MCV
T3579	13267-13270	CC	denotes	and
T3580	13271-13276	JJR	denotes	lower
T3582	13277-13284	JJ	denotes	osmotic
T3581	13285-13295	NN	denotes	resistance
T3583	13296-13297	-LRB-	denotes	[
T3584	13297-13299	CD	denotes	33
T3585	13299-13300	-RRB-	denotes	]
T3586	13300-13301	.	denotes	.
T3587	13301-13391	sentence	denotes	Similar properties can be observed in red blood cells with lowered membrane permeability.
T3588	13302-13309	JJ	denotes	Similar
T3589	13310-13320	NNS	denotes	properties
T3591	13321-13324	MD	denotes	can
T3592	13325-13327	VB	denotes	be
T3590	13328-13336	VBN	denotes	observed
T3593	13337-13339	IN	denotes	in
T3594	13340-13343	JJ	denotes	red
T3596	13344-13349	NN	denotes	blood
T3595	13350-13355	NNS	denotes	cells
T3597	13356-13360	IN	denotes	with
T3598	13361-13368	VBN	denotes	lowered
T3600	13369-13377	NN	denotes	membrane
T3599	13378-13390	NN	denotes	permeability
T3601	13390-13391	.	denotes	.
T3602	13391-13510	sentence	denotes	The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to wild type cells.
T3603	13392-13395	DT	denotes	The
T3605	13396-13403	JJ	denotes	osmotic
T3604	13404-13414	NN	denotes	resistance
T3607	13415-13417	IN	denotes	of
T3608	13418-13425	NN	denotes	annexin
T3609	13426-13428	NN	denotes	A7
T3610	13429-13438	JJ	denotes	deficient
T3612	13439-13442	JJ	denotes	red
T3613	13443-13448	NN	denotes	blood
T3611	13449-13454	NNS	denotes	cells
T3606	13455-13457	VBZ	denotes	is
T3614	13458-13471	RB	denotes	significantly
T3615	13472-13481	VBN	denotes	increased
T3616	13482-13490	VBN	denotes	compared
T3617	13491-13493	IN	denotes	to
T3618	13494-13498	JJ	denotes	wild
T3619	13499-13503	NN	denotes	type
T3620	13504-13509	NNS	denotes	cells
T3621	13509-13510	.	denotes	.
T3622	13510-13631	sentence	denotes	The minimal resistance value of knock out cells is observed in a 0.60 % NaCl solution versus 0.65 % for wild type cells.
T3623	13511-13514	DT	denotes	The
T3625	13515-13522	JJ	denotes	minimal
T3626	13523-13533	NN	denotes	resistance
T3624	13534-13539	NN	denotes	value
T3628	13540-13542	IN	denotes	of
T3629	13543-13548	NN	denotes	knock
T3630	13549-13552	NN	denotes	out
T3631	13553-13558	NNS	denotes	cells
T3632	13559-13561	VBZ	denotes	is
T3627	13562-13570	VBN	denotes	observed
T3633	13571-13573	IN	denotes	in
T3634	13574-13575	DT	denotes	a
T3636	13576-13580	CD	denotes	0.60
T3637	13581-13582	NN	denotes	%
T3638	13583-13587	NN	denotes	NaCl
T3635	13588-13596	NN	denotes	solution
T3639	13597-13603	CC	denotes	versus
T3640	13604-13608	CD	denotes	0.65
T3641	13609-13610	NN	denotes	%
T3642	13611-13614	IN	denotes	for
T3643	13615-13619	JJ	denotes	wild
T3644	13620-13624	NN	denotes	type
T3645	13625-13630	NNS	denotes	cells
T3646	13630-13631	.	denotes	.
T3647	13631-13781	sentence	denotes	50 % haemolysis is achieved at 0.516 % NaCl solution for knock out versus 0.564 % NaCl for wild type cells (mean values, p = 0.00066, n = 8; Fig. 5).
T3648	13632-13634	CD	denotes	50
T3649	13635-13636	NN	denotes	%
T3650	13637-13647	NN	denotes	haemolysis
T3652	13648-13650	VBZ	denotes	is
T3651	13651-13659	VBN	denotes	achieved
T3653	13660-13662	IN	denotes	at
T3654	13663-13668	CD	denotes	0.516
T3655	13669-13670	NN	denotes	%
T3657	13671-13675	NN	denotes	NaCl
T3656	13676-13684	NN	denotes	solution
T3658	13685-13688	IN	denotes	for
T3659	13689-13694	NN	denotes	knock
T3660	13695-13698	NN	denotes	out
T3661	13699-13705	CC	denotes	versus
T3662	13706-13711	CD	denotes	0.564
T3663	13712-13713	NN	denotes	%
T3664	13714-13718	NN	denotes	NaCl
T3665	13719-13722	IN	denotes	for
T3666	13723-13727	JJ	denotes	wild
T3667	13728-13732	NN	denotes	type
T3668	13733-13738	NNS	denotes	cells
T3669	13739-13740	-LRB-	denotes	(
T3671	13740-13744	NN	denotes	mean
T3672	13745-13751	NNS	denotes	values
T3673	13751-13753	,	denotes	,
T3674	13753-13754	NN	denotes	p
T3676	13755-13756	SYM	denotes	=
T3675	13757-13764	CD	denotes	0.00066
T3677	13764-13766	,	denotes	,
T3678	13766-13767	NN	denotes	n
T3680	13768-13769	SYM	denotes	=
T3679	13770-13771	CD	denotes	8
T3681	13771-13772	:	denotes	;
T3670	13773-13777	NN	denotes	Fig.
T3682	13778-13779	CD	denotes	5
T3683	13779-13780	-RRB-	denotes	)
T3684	13780-13781	.	denotes	.
T3685	13781-13885	sentence	denotes	The resistance width of the knock out cells is slightly lower (0.20 % instead of 0.25 % NaCl solution).
T3686	13782-13785	DT	denotes	The
T3688	13786-13796	NN	denotes	resistance
T3687	13797-13802	NN	denotes	width
T3690	13803-13805	IN	denotes	of
T3691	13806-13809	DT	denotes	the
T3693	13810-13815	NN	denotes	knock
T3694	13816-13819	NN	denotes	out
T3692	13820-13825	NNS	denotes	cells
T3689	13826-13828	VBZ	denotes	is
T3695	13829-13837	RB	denotes	slightly
T3696	13838-13843	JJR	denotes	lower
T3697	13844-13845	-LRB-	denotes	(
T3699	13845-13849	CD	denotes	0.20
T3700	13850-13851	NN	denotes	%
T3702	13852-13859	RB	denotes	instead
T3703	13860-13862	IN	denotes	of
T3704	13863-13867	CD	denotes	0.25
T3705	13868-13869	NN	denotes	%
T3701	13870-13874	NN	denotes	NaCl
T3698	13875-13883	NN	denotes	solution
T3706	13883-13884	-RRB-	denotes	)
T3707	13884-13885	.	denotes	.
T3708	13885-14050	sentence	denotes	We also performed direct measurements of the red blood cell membrane deformability, to further characterize a possible role of annexin A7 on the membrane stability.
T3709	13886-13888	PRP	denotes	We
T3711	13889-13893	RB	denotes	also
T3710	13894-13903	VBD	denotes	performed
T3712	13904-13910	JJ	denotes	direct
T3713	13911-13923	NNS	denotes	measurements
T3714	13924-13926	IN	denotes	of
T3715	13927-13930	DT	denotes	the
T3717	13931-13934	JJ	denotes	red
T3719	13935-13940	NN	denotes	blood
T3718	13941-13945	NN	denotes	cell
T3720	13946-13954	NN	denotes	membrane
T3716	13955-13968	NN	denotes	deformability
T3721	13968-13970	,	denotes	,
T3722	13970-13972	TO	denotes	to
T3724	13973-13980	RBR	denotes	further
T3723	13981-13993	VB	denotes	characterize
T3725	13994-13995	DT	denotes	a
T3727	13996-14004	JJ	denotes	possible
T3726	14005-14009	NN	denotes	role
T3728	14010-14012	IN	denotes	of
T3729	14013-14020	NN	denotes	annexin
T3730	14021-14023	NN	denotes	A7
T3731	14024-14026	IN	denotes	on
T3732	14027-14030	DT	denotes	the
T3734	14031-14039	NN	denotes	membrane
T3733	14040-14049	NN	denotes	stability
T3735	14049-14050	.	denotes	.
T3736	14050-14175	sentence	denotes	With micropipette experiments we tried to correlate mechanical characteristics of the red blood cells with their morphology.
T3737	14051-14055	IN	denotes	With
T3739	14056-14068	NN	denotes	micropipette
T3740	14069-14080	NNS	denotes	experiments
T3741	14081-14083	PRP	denotes	we
T3738	14084-14089	VBD	denotes	tried
T3742	14090-14092	TO	denotes	to
T3743	14093-14102	VB	denotes	correlate
T3744	14103-14113	JJ	denotes	mechanical
T3745	14114-14129	NNS	denotes	characteristics
T3746	14130-14132	IN	denotes	of
T3747	14133-14136	DT	denotes	the
T3749	14137-14140	JJ	denotes	red
T3750	14141-14146	NN	denotes	blood
T3748	14147-14152	NNS	denotes	cells
T3751	14153-14157	IN	denotes	with
T3752	14158-14163	PRP$	denotes	their
T3753	14164-14174	NN	denotes	morphology
T3754	14174-14175	.	denotes	.
T3755	14175-14372	sentence	denotes	We could not observe any statistically significant difference of values describing membrane rigidity and lysis force in these experiments (M. Heil, B. Hoffmann and R. Merkel, unpublished results).
T3756	14176-14178	PRP	denotes	We
T3758	14179-14184	MD	denotes	could
T3759	14185-14188	RB	denotes	not
T3757	14189-14196	VB	denotes	observe
T3760	14197-14200	DT	denotes	any
T3762	14201-14214	RB	denotes	statistically
T3763	14215-14226	JJ	denotes	significant
T3761	14227-14237	NN	denotes	difference
T3764	14238-14240	IN	denotes	of
T3765	14241-14247	NNS	denotes	values
T3766	14248-14258	VBG	denotes	describing
T3767	14259-14267	NN	denotes	membrane
T3768	14268-14276	NN	denotes	rigidity
T3769	14277-14280	CC	denotes	and
T3770	14281-14286	NN	denotes	lysis
T3771	14287-14292	NN	denotes	force
T3772	14293-14295	IN	denotes	in
T3773	14296-14301	DT	denotes	these
T3774	14302-14313	NNS	denotes	experiments
T3775	14314-14315	-LRB-	denotes	(
T3776	14315-14317	NNP	denotes	M.
T3777	14318-14322	NNP	denotes	Heil
T3778	14322-14324	,	denotes	,
T3779	14324-14326	NNP	denotes	B.
T3780	14327-14335	NNP	denotes	Hoffmann
T3781	14336-14339	CC	denotes	and
T3782	14340-14342	NNP	denotes	R.
T3783	14343-14349	NNP	denotes	Merkel
T3784	14349-14351	,	denotes	,
T3785	14351-14362	JJ	denotes	unpublished
T3786	14363-14370	NNS	denotes	results
T3787	14370-14371	-RRB-	denotes	)
T3788	14371-14372	.	denotes	.
T3789	14372-14542	sentence	denotes	However, the distinction observed in the osmotic resistance experiments was highly significant and reflects a mean value over a high number of different red blood cells.
T3790	14373-14380	RB	denotes	However
T3792	14380-14382	,	denotes	,
T3793	14382-14385	DT	denotes	the
T3794	14386-14397	NN	denotes	distinction
T3795	14398-14406	VBN	denotes	observed
T3796	14407-14409	IN	denotes	in
T3797	14410-14413	DT	denotes	the
T3799	14414-14421	JJ	denotes	osmotic
T3800	14422-14432	NN	denotes	resistance
T3798	14433-14444	NNS	denotes	experiments
T3791	14445-14448	VBD	denotes	was
T3801	14449-14455	RB	denotes	highly
T3802	14456-14467	JJ	denotes	significant
T3803	14468-14471	CC	denotes	and
T3804	14472-14480	VBZ	denotes	reflects
T3805	14481-14482	DT	denotes	a
T3807	14483-14487	JJ	denotes	mean
T3806	14488-14493	NN	denotes	value
T3808	14494-14498	IN	denotes	over
T3809	14499-14500	DT	denotes	a
T3811	14501-14505	JJ	denotes	high
T3810	14506-14512	NN	denotes	number
T3812	14513-14515	IN	denotes	of
T3813	14516-14525	JJ	denotes	different
T3815	14526-14529	JJ	denotes	red
T3816	14530-14535	NN	denotes	blood
T3814	14536-14541	NNS	denotes	cells
T3817	14541-14542	.	denotes	.
T3818	14542-15200	sentence	denotes	Figure 5 Osmotic resistance curves of red blood cells from wild type and anxA7-/- mutant mice. The osmotic resistance towards changes in the extracellular ionic strength is a convenient assay for analysis of the red blood cell integrity. It is measured as a sodium chloride concentration in which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of a red blood cell. The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to the one of wild type (p = 0.00066; n = 8). These data suggest that annexin A7 contributes to the shape of red blood cells and the osmotic resistance.
T9617	14553-14560	JJ	denotes	Osmotic
T9618	14561-14571	NN	denotes	resistance
T9619	14572-14578	NNS	denotes	curves
T9620	14579-14581	IN	denotes	of
T9621	14582-14585	JJ	denotes	red
T9623	14586-14591	NN	denotes	blood
T9622	14592-14597	NNS	denotes	cells
T9624	14598-14602	IN	denotes	from
T9625	14603-14607	JJ	denotes	wild
T9626	14608-14612	NN	denotes	type
T9628	14613-14616	CC	denotes	and
T9629	14617-14622	NN	denotes	anxA7
T9630	14622-14623	SYM	denotes	-
T9631	14623-14624	HYPH	denotes	/
T9632	14624-14625	SYM	denotes	-
T9633	14626-14632	JJ	denotes	mutant
T9627	14633-14637	NNS	denotes	mice
T9634	14637-14638	.	denotes	.
T9635	14638-14781	sentence	denotes	The osmotic resistance towards changes in the extracellular ionic strength is a convenient assay for analysis of the red blood cell integrity.
T9636	14639-14642	DT	denotes	The
T9638	14643-14650	JJ	denotes	osmotic
T9637	14651-14661	NN	denotes	resistance
T9640	14662-14669	IN	denotes	towards
T9641	14670-14677	NNS	denotes	changes
T9642	14678-14680	IN	denotes	in
T9643	14681-14684	DT	denotes	the
T9645	14685-14698	JJ	denotes	extracellular
T9646	14699-14704	JJ	denotes	ionic
T9644	14705-14713	NN	denotes	strength
T9639	14714-14716	VBZ	denotes	is
T9647	14717-14718	DT	denotes	a
T9649	14719-14729	JJ	denotes	convenient
T9648	14730-14735	NN	denotes	assay
T9650	14736-14739	IN	denotes	for
T9651	14740-14748	NN	denotes	analysis
T9652	14749-14751	IN	denotes	of
T9653	14752-14755	DT	denotes	the
T9655	14756-14759	JJ	denotes	red
T9657	14760-14765	NN	denotes	blood
T9656	14766-14770	NN	denotes	cell
T9654	14771-14780	NN	denotes	integrity
T9658	14780-14781	.	denotes	.
T9659	14781-14948	sentence	denotes	It is measured as a sodium chloride concentration in which cellular lysis starts (minimal resistance) up to a complete lysis (maximal resistance) of a red blood cell.
T9660	14782-14784	PRP	denotes	It
T9662	14785-14787	VBZ	denotes	is
T9661	14788-14796	VBN	denotes	measured
T9663	14797-14799	IN	denotes	as
T9664	14800-14801	DT	denotes	a
T9666	14802-14808	NN	denotes	sodium
T9667	14809-14817	NN	denotes	chloride
T9665	14818-14831	NN	denotes	concentration
T9668	14832-14834	IN	denotes	in
T9670	14835-14840	WDT	denotes	which
T9671	14841-14849	JJ	denotes	cellular
T9672	14850-14855	NN	denotes	lysis
T9669	14856-14862	VBZ	denotes	starts
T9673	14863-14864	-LRB-	denotes	(
T9675	14864-14871	JJ	denotes	minimal
T9674	14872-14882	NN	denotes	resistance
T9676	14882-14883	-RRB-	denotes	)
T9677	14884-14886	IN	denotes	up
T9678	14887-14889	IN	denotes	to
T9679	14890-14891	DT	denotes	a
T9681	14892-14900	JJ	denotes	complete
T9680	14901-14906	NN	denotes	lysis
T9682	14907-14908	-LRB-	denotes	(
T9684	14908-14915	JJ	denotes	maximal
T9683	14916-14926	NN	denotes	resistance
T9685	14926-14927	-RRB-	denotes	)
T9686	14928-14930	IN	denotes	of
T9687	14931-14932	DT	denotes	a
T9689	14933-14936	JJ	denotes	red
T9690	14937-14942	NN	denotes	blood
T9688	14943-14947	NN	denotes	cell
T9691	14947-14948	.	denotes	.
T9692	14948-15093	sentence	denotes	The osmotic resistance of annexin A7 deficient red blood cells is significantly increased compared to the one of wild type (p = 0.00066; n = 8).
T9693	14949-14952	DT	denotes	The
T9695	14953-14960	JJ	denotes	osmotic
T9694	14961-14971	NN	denotes	resistance
T9697	14972-14974	IN	denotes	of
T9698	14975-14982	NN	denotes	annexin
T9699	14983-14985	NN	denotes	A7
T9700	14986-14995	JJ	denotes	deficient
T9702	14996-14999	JJ	denotes	red
T9703	15000-15005	NN	denotes	blood
T9701	15006-15011	NNS	denotes	cells
T9696	15012-15014	VBZ	denotes	is
T9704	15015-15028	RB	denotes	significantly
T9705	15029-15038	JJ	denotes	increased
T9706	15039-15047	VBN	denotes	compared
T9707	15048-15050	IN	denotes	to
T9708	15051-15054	DT	denotes	the
T9709	15055-15058	CD	denotes	one
T9710	15059-15061	IN	denotes	of
T9711	15062-15066	JJ	denotes	wild
T9712	15067-15071	NN	denotes	type
T9713	15072-15073	-LRB-	denotes	(
T9715	15073-15074	NN	denotes	p
T9717	15075-15076	SYM	denotes	=
T9716	15077-15084	CD	denotes	0.00066
T9718	15084-15085	:	denotes	;
T9719	15086-15087	NN	denotes	n
T9720	15088-15089	SYM	denotes	=
T9714	15090-15091	CD	denotes	8
T9721	15091-15092	-RRB-	denotes	)
T9722	15092-15093	.	denotes	.
T3819	15094-15099	DT	denotes	These
T3820	15100-15104	NNS	denotes	data
T3821	15105-15112	VBP	denotes	suggest
T3822	15113-15117	IN	denotes	that
T3824	15118-15125	NN	denotes	annexin
T3825	15126-15128	NN	denotes	A7
T3823	15129-15140	VBZ	denotes	contributes
T3826	15141-15143	IN	denotes	to
T3827	15144-15147	DT	denotes	the
T3828	15148-15153	NN	denotes	shape
T3829	15154-15156	IN	denotes	of
T3830	15157-15160	JJ	denotes	red
T3832	15161-15166	NN	denotes	blood
T3831	15167-15172	NNS	denotes	cells
T3833	15173-15176	CC	denotes	and
T3834	15177-15180	DT	denotes	the
T3836	15181-15188	JJ	denotes	osmotic
T3835	15189-15199	NN	denotes	resistance
T3837	15199-15200	.	denotes	.
T3838	15200-15378	sentence	denotes	As we have not observed a binding of annexin A7 to F-actin (data not shown) it could do so either by alteration of the membrane rigidity and/or by affecting the ion homeostasis.
T3839	15201-15203	IN	denotes	As
T3841	15204-15206	PRP	denotes	we
T3842	15207-15211	VBP	denotes	have
T3843	15212-15215	RB	denotes	not
T3840	15216-15224	VBN	denotes	observed
T3845	15225-15226	DT	denotes	a
T3846	15227-15234	NN	denotes	binding
T3847	15235-15237	IN	denotes	of
T3848	15238-15245	NN	denotes	annexin
T3849	15246-15248	NN	denotes	A7
T3850	15249-15251	IN	denotes	to
T3851	15252-15253	NN	denotes	F
T3853	15253-15254	HYPH	denotes	-
T3852	15254-15259	NN	denotes	actin
T3854	15260-15261	-LRB-	denotes	(
T3856	15261-15265	NNS	denotes	data
T3857	15266-15269	RB	denotes	not
T3855	15270-15275	VBN	denotes	shown
T3858	15275-15276	-RRB-	denotes	)
T3859	15277-15279	PRP	denotes	it
T3860	15280-15285	MD	denotes	could
T3844	15286-15288	VB	denotes	do
T3861	15289-15291	RB	denotes	so
T3862	15292-15298	CC	denotes	either
T3863	15299-15301	IN	denotes	by
T3864	15302-15312	NN	denotes	alteration
T3865	15313-15315	IN	denotes	of
T3866	15316-15319	DT	denotes	the
T3868	15320-15328	NN	denotes	membrane
T3867	15329-15337	NN	denotes	rigidity
T3869	15338-15341	CC	denotes	and
T3870	15341-15342	HYPH	denotes	/
T3871	15342-15344	CC	denotes	or
T3872	15345-15347	IN	denotes	by
T3873	15348-15357	VBG	denotes	affecting
T3874	15358-15361	DT	denotes	the
T3876	15362-15365	NN	denotes	ion
T3875	15366-15377	NN	denotes	homeostasis
T3877	15377-15378	.	denotes	.
T4078	15380-15383	DT	denotes	The
T4079	15384-15388	NN	denotes	lack
T4081	15389-15391	IN	denotes	of
T4082	15392-15399	NN	denotes	annexin
T4083	15400-15402	NN	denotes	A7
T4080	15403-15410	VBZ	denotes	affects
T4084	15411-15418	JJ	denotes	primary
T4085	15419-15430	NN	denotes	haemostasis
T4086	15431-15433	FW	denotes	ex
T4087	15434-15438	FW	denotes	vivo
T4088	15438-15602	sentence	denotes	The use of an advanced electronic cell counter/flow cytometer (ADVIA 120) allowed us to screen other parameters as well despite of the limited murine blood volume.
T4089	15439-15442	DT	denotes	The
T4090	15443-15446	NN	denotes	use
T4092	15447-15449	IN	denotes	of
T4093	15450-15452	DT	denotes	an
T4095	15453-15461	JJ	denotes	advanced
T4096	15462-15472	JJ	denotes	electronic
T4097	15473-15477	NN	denotes	cell
T4094	15478-15485	NN	denotes	counter
T4098	15485-15486	HYPH	denotes	/
T4099	15486-15490	NN	denotes	flow
T4100	15491-15500	NN	denotes	cytometer
T4101	15501-15502	-LRB-	denotes	(
T4102	15502-15507	NN	denotes	ADVIA
T4103	15508-15511	CD	denotes	120
T4104	15511-15512	-RRB-	denotes	)
T4091	15513-15520	VBD	denotes	allowed
T4105	15521-15523	PRP	denotes	us
T4107	15524-15526	TO	denotes	to
T4106	15527-15533	VB	denotes	screen
T4108	15534-15539	JJ	denotes	other
T4109	15540-15550	NNS	denotes	parameters
T4110	15551-15553	RB	denotes	as
T4111	15554-15558	RB	denotes	well
T4112	15559-15566	IN	denotes	despite
T4113	15567-15569	IN	denotes	of
T4114	15570-15573	DT	denotes	the
T4116	15574-15581	JJ	denotes	limited
T4117	15582-15588	JJ	denotes	murine
T4118	15589-15594	NN	denotes	blood
T4115	15595-15601	NN	denotes	volume
T4119	15601-15602	.	denotes	.
T4120	15602-15845	sentence	denotes	We analysed leukocytes, red blood cells, haemoglobin content, haematocrit, mean cellular volume, mean cellular haemoglobin, mean cellular haemoglobin concentration, platelets, neutrophiles, lymphocytes, monocytes, eosinophiles and basophiles.
T4121	15603-15605	PRP	denotes	We
T4122	15606-15614	VBD	denotes	analysed
T4123	15615-15625	NNS	denotes	leukocytes
T4124	15625-15627	,	denotes	,
T4125	15627-15630	JJ	denotes	red
T4127	15631-15636	NN	denotes	blood
T4126	15637-15642	NNS	denotes	cells
T4128	15642-15644	,	denotes	,
T4129	15644-15655	NN	denotes	haemoglobin
T4130	15656-15663	NN	denotes	content
T4131	15663-15665	,	denotes	,
T4132	15665-15676	NN	denotes	haematocrit
T4133	15676-15678	,	denotes	,
T4134	15678-15682	JJ	denotes	mean
T4136	15683-15691	JJ	denotes	cellular
T4135	15692-15698	NN	denotes	volume
T4137	15698-15700	,	denotes	,
T4138	15700-15704	JJ	denotes	mean
T4140	15705-15713	JJ	denotes	cellular
T4139	15714-15725	NN	denotes	haemoglobin
T4141	15725-15727	,	denotes	,
T4142	15727-15731	JJ	denotes	mean
T4144	15732-15740	JJ	denotes	cellular
T4143	15741-15752	NN	denotes	haemoglobin
T4145	15753-15766	NN	denotes	concentration
T4146	15766-15768	,	denotes	,
T4147	15768-15777	NNS	denotes	platelets
T4148	15777-15779	,	denotes	,
T4149	15779-15791	NNS	denotes	neutrophiles
T4150	15791-15793	,	denotes	,
T4151	15793-15804	NNS	denotes	lymphocytes
T4152	15804-15806	,	denotes	,
T4153	15806-15815	NNS	denotes	monocytes
T4154	15815-15817	,	denotes	,
T4155	15817-15829	NNS	denotes	eosinophiles
T4156	15830-15833	CC	denotes	and
T4157	15834-15844	NNS	denotes	basophiles
T4158	15844-15845	.	denotes	.
T4159	15845-15986	sentence	denotes	In these tests of whole blood we found only a change in the platelet numbers, whereas all other haematological parameters were not affected.
T4160	15846-15848	IN	denotes	In
T4162	15849-15854	DT	denotes	these
T4163	15855-15860	NNS	denotes	tests
T4164	15861-15863	IN	denotes	of
T4165	15864-15869	JJ	denotes	whole
T4166	15870-15875	NN	denotes	blood
T4167	15876-15878	PRP	denotes	we
T4161	15879-15884	VBD	denotes	found
T4168	15885-15889	RB	denotes	only
T4169	15890-15891	DT	denotes	a
T4170	15892-15898	NN	denotes	change
T4171	15899-15901	IN	denotes	in
T4172	15902-15905	DT	denotes	the
T4174	15906-15914	NN	denotes	platelet
T4173	15915-15922	NNS	denotes	numbers
T4175	15922-15924	,	denotes	,
T4176	15924-15931	IN	denotes	whereas
T4178	15932-15935	DT	denotes	all
T4180	15936-15941	JJ	denotes	other
T4181	15942-15956	JJ	denotes	haematological
T4179	15957-15967	NNS	denotes	parameters
T4182	15968-15972	VBD	denotes	were
T4183	15973-15976	RB	denotes	not
T4177	15977-15985	VBN	denotes	affected
T4184	15985-15986	.	denotes	.
T4185	15986-16114	sentence	denotes	The mean platelet counts from wild type and anxA7-/- mutant mice are determined as 674 × 103/μl and 774 × 103/μl, respectively.
T4186	15987-15990	DT	denotes	The
T4188	15991-15995	JJ	denotes	mean
T4189	15996-16004	NN	denotes	platelet
T4187	16005-16011	NNS	denotes	counts
T4191	16012-16016	IN	denotes	from
T4192	16017-16021	JJ	denotes	wild
T4193	16022-16026	NN	denotes	type
T4195	16027-16030	CC	denotes	and
T4196	16031-16036	NN	denotes	anxA7
T4197	16036-16037	SYM	denotes	-
T4198	16037-16038	HYPH	denotes	/
T4199	16038-16039	SYM	denotes	-
T4200	16040-16046	JJ	denotes	mutant
T4194	16047-16051	NNS	denotes	mice
T4201	16052-16055	VBP	denotes	are
T4190	16056-16066	VBN	denotes	determined
T4202	16067-16069	IN	denotes	as
T4203	16070-16073	CD	denotes	674
T4205	16074-16075	SYM	denotes	×
T4204	16076-16079	CD	denotes	103
T4206	16079-16080	SYM	denotes	/
T4207	16080-16082	NNS	denotes	μl
T4208	16083-16086	CC	denotes	and
T4209	16087-16090	CD	denotes	774
T4211	16091-16092	SYM	denotes	×
T4210	16093-16096	CD	denotes	103
T4212	16096-16097	SYM	denotes	/
T4213	16097-16099	NNS	denotes	μl
T4214	16099-16101	,	denotes	,
T4215	16101-16113	RB	denotes	respectively
T4216	16113-16114	.	denotes	.
T4217	16114-16227	sentence	denotes	The platelet counts in knock out mice are significantly higher (p = 0.0275; n knock out = 11, n wild type = 14).
T4218	16115-16118	DT	denotes	The
T4220	16119-16127	NN	denotes	platelet
T4219	16128-16134	NNS	denotes	counts
T4222	16135-16137	IN	denotes	in
T4223	16138-16143	NN	denotes	knock
T4224	16144-16147	NN	denotes	out
T4225	16148-16152	NNS	denotes	mice
T4221	16153-16156	VBP	denotes	are
T4226	16157-16170	RB	denotes	significantly
T4227	16171-16177	JJR	denotes	higher
T4228	16178-16179	-LRB-	denotes	(
T4230	16179-16180	NN	denotes	p
T4232	16181-16182	SYM	denotes	=
T4231	16183-16189	CD	denotes	0.0275
T4233	16189-16190	:	denotes	;
T4234	16191-16192	NN	denotes	n
T4236	16193-16198	NN	denotes	knock
T4235	16199-16202	NN	denotes	out
T4238	16203-16204	SYM	denotes	=
T4237	16205-16207	CD	denotes	11
T4239	16207-16209	,	denotes	,
T4240	16209-16210	NN	denotes	n
T4242	16211-16215	JJ	denotes	wild
T4241	16216-16220	NN	denotes	type
T4243	16221-16222	SYM	denotes	=
T4229	16223-16225	CD	denotes	14
T4244	16225-16226	-RRB-	denotes	)
T4245	16226-16227	.	denotes	.
T4246	16227-16289	sentence	denotes	An increase in platelet counts is a rather uncommon disorder.
T4247	16228-16230	DT	denotes	An
T4248	16231-16239	NN	denotes	increase
T4250	16240-16242	IN	denotes	in
T4251	16243-16251	NN	denotes	platelet
T4252	16252-16258	NNS	denotes	counts
T4249	16259-16261	VBZ	denotes	is
T4253	16262-16263	DT	denotes	a
T4255	16264-16270	RB	denotes	rather
T4256	16271-16279	JJ	denotes	uncommon
T4254	16280-16288	NN	denotes	disorder
T4257	16288-16289	.	denotes	.
T4258	16289-16465	sentence	denotes	In humans, platelet counts normally increase only transiently as in reactive thrombocytosis and under postoperative conditions or are largely increased in neoplastic diseases.
T4259	16290-16292	IN	denotes	In
T4261	16293-16299	NNS	denotes	humans
T4262	16299-16301	,	denotes	,
T4263	16301-16309	NN	denotes	platelet
T4264	16310-16316	NNS	denotes	counts
T4265	16317-16325	RB	denotes	normally
T4260	16326-16334	VBP	denotes	increase
T4266	16335-16339	RB	denotes	only
T4267	16340-16351	RB	denotes	transiently
T4268	16352-16354	IN	denotes	as
T4269	16355-16357	IN	denotes	in
T4270	16358-16366	JJ	denotes	reactive
T4271	16367-16381	NN	denotes	thrombocytosis
T4272	16382-16385	CC	denotes	and
T4273	16386-16391	IN	denotes	under
T4274	16392-16405	JJ	denotes	postoperative
T4275	16406-16416	NNS	denotes	conditions
T4276	16417-16419	CC	denotes	or
T4277	16420-16423	VBP	denotes	are
T4279	16424-16431	RB	denotes	largely
T4278	16432-16441	VBN	denotes	increased
T4280	16442-16444	IN	denotes	in
T4281	16445-16455	JJ	denotes	neoplastic
T4282	16456-16464	NNS	denotes	diseases
T4283	16464-16465	.	denotes	.
T4284	16465-16519	sentence	denotes	By contrast, the anxA7-/-mice are healthy and viable.
T4285	16466-16468	IN	denotes	By
T4287	16469-16477	NN	denotes	contrast
T4288	16477-16479	,	denotes	,
T4289	16479-16482	DT	denotes	the
T4291	16483-16488	NN	denotes	anxA7
T4292	16488-16489	SYM	denotes	-
T4293	16489-16490	HYPH	denotes	/
T4294	16490-16491	SYM	denotes	-
T4290	16491-16495	NNS	denotes	mice
T4286	16496-16499	VBP	denotes	are
T4295	16500-16507	JJ	denotes	healthy
T4296	16508-16511	CC	denotes	and
T4297	16512-16518	JJ	denotes	viable
T4298	16518-16519	.	denotes	.
T4299	16519-16628	sentence	denotes	Therefore we tested the platelet function and performed aggregometry measurements with platelet rich plasma.
T4300	16520-16529	RB	denotes	Therefore
T4302	16530-16532	PRP	denotes	we
T4301	16533-16539	VBD	denotes	tested
T4303	16540-16543	DT	denotes	the
T4305	16544-16552	NN	denotes	platelet
T4304	16553-16561	NN	denotes	function
T4306	16562-16565	CC	denotes	and
T4307	16566-16575	VBD	denotes	performed
T4308	16576-16588	NN	denotes	aggregometry
T4309	16589-16601	NNS	denotes	measurements
T4310	16602-16606	IN	denotes	with
T4311	16607-16615	NN	denotes	platelet
T4312	16616-16620	JJ	denotes	rich
T4313	16621-16627	NN	denotes	plasma
T4314	16627-16628	.	denotes	.
T4315	16628-16763	sentence	denotes	Platelet activation is observed as a morphological change from the resting discoid state to activated spherical cells with pseudopods.
T4316	16629-16637	NN	denotes	Platelet
T4317	16638-16648	NN	denotes	activation
T4319	16649-16651	VBZ	denotes	is
T4318	16652-16660	VBN	denotes	observed
T4320	16661-16663	IN	denotes	as
T4321	16664-16665	DT	denotes	a
T4323	16666-16679	JJ	denotes	morphological
T4322	16680-16686	NN	denotes	change
T4324	16687-16691	IN	denotes	from
T4325	16692-16695	DT	denotes	the
T4327	16696-16703	VBG	denotes	resting
T4328	16704-16711	JJ	denotes	discoid
T4326	16712-16717	NN	denotes	state
T4329	16718-16720	IN	denotes	to
T4330	16721-16730	VBN	denotes	activated
T4332	16731-16740	JJ	denotes	spherical
T4331	16741-16746	NNS	denotes	cells
T4333	16747-16751	IN	denotes	with
T4334	16752-16762	NNS	denotes	pseudopods
T4335	16762-16763	.	denotes	.
T4336	16763-16829	sentence	denotes	The morphological changes are due to cytoskeletal rearrangements.
T4337	16764-16767	DT	denotes	The
T4339	16768-16781	JJ	denotes	morphological
T4338	16782-16789	NNS	denotes	changes
T4340	16790-16793	VBP	denotes	are
T4341	16794-16797	IN	denotes	due
T4342	16798-16800	IN	denotes	to
T4343	16801-16813	JJ	denotes	cytoskeletal
T4344	16814-16828	NNS	denotes	rearrangements
T4345	16828-16829	.	denotes	.
T4346	16829-16951	sentence	denotes	In vitro the activated cells form aggregates recruiting additional cells in the solution thereby reducing its cloudiness.
T4347	16830-16832	FW	denotes	In
T4348	16833-16838	FW	denotes	vitro
T4350	16839-16842	DT	denotes	the
T4352	16843-16852	VBN	denotes	activated
T4351	16853-16858	NNS	denotes	cells
T4349	16859-16863	VBP	denotes	form
T4353	16864-16874	NNS	denotes	aggregates
T4354	16875-16885	VBG	denotes	recruiting
T4355	16886-16896	JJ	denotes	additional
T4356	16897-16902	NNS	denotes	cells
T4357	16903-16905	IN	denotes	in
T4358	16906-16909	DT	denotes	the
T4359	16910-16918	NN	denotes	solution
T4360	16919-16926	RB	denotes	thereby
T4361	16927-16935	VBG	denotes	reducing
T4362	16936-16939	PRP$	denotes	its
T4363	16940-16950	NN	denotes	cloudiness
T4364	16950-16951	.	denotes	.
T4365	16951-17250	sentence	denotes	Analysis of the transmission values of aggregation curves after platelet activation by ristocetin addition (von Willebrand cofactor) showed that both curves differed significantly at the time point of seven seconds after platelet initiation (p = 0.0287, n knock out = 16, n wild type = 15; Fig. 6).
T4366	16952-16960	NN	denotes	Analysis
T4368	16961-16963	IN	denotes	of
T4369	16964-16967	DT	denotes	the
T4371	16968-16980	NN	denotes	transmission
T4370	16981-16987	NNS	denotes	values
T4372	16988-16990	IN	denotes	of
T4373	16991-17002	NN	denotes	aggregation
T4374	17003-17009	NNS	denotes	curves
T4375	17010-17015	IN	denotes	after
T4376	17016-17024	NN	denotes	platelet
T4377	17025-17035	NN	denotes	activation
T4378	17036-17038	IN	denotes	by
T4379	17039-17049	NN	denotes	ristocetin
T4380	17050-17058	NN	denotes	addition
T4381	17059-17060	-LRB-	denotes	(
T4383	17060-17063	NN	denotes	von
T4384	17064-17074	NN	denotes	Willebrand
T4382	17075-17083	NN	denotes	cofactor
T4385	17083-17084	-RRB-	denotes	)
T4367	17085-17091	VBD	denotes	showed
T4386	17092-17096	IN	denotes	that
T4388	17097-17101	DT	denotes	both
T4389	17102-17108	NNS	denotes	curves
T4387	17109-17117	VBD	denotes	differed
T4390	17118-17131	RB	denotes	significantly
T4391	17132-17134	IN	denotes	at
T4392	17135-17138	DT	denotes	the
T4394	17139-17143	NN	denotes	time
T4393	17144-17149	NN	denotes	point
T4395	17150-17152	IN	denotes	of
T4396	17153-17158	CD	denotes	seven
T4397	17159-17166	NNS	denotes	seconds
T4398	17167-17172	IN	denotes	after
T4399	17173-17181	NN	denotes	platelet
T4400	17182-17192	NN	denotes	initiation
T4401	17193-17194	-LRB-	denotes	(
T4403	17194-17195	NN	denotes	p
T4405	17196-17197	SYM	denotes	=
T4404	17198-17204	CD	denotes	0.0287
T4406	17204-17206	,	denotes	,
T4407	17206-17207	NN	denotes	n
T4409	17208-17213	NN	denotes	knock
T4408	17214-17217	NN	denotes	out
T4411	17218-17219	SYM	denotes	=
T4410	17220-17222	CD	denotes	16
T4412	17222-17224	,	denotes	,
T4413	17224-17225	NN	denotes	n
T4415	17226-17230	JJ	denotes	wild
T4414	17231-17235	NN	denotes	type
T4417	17236-17237	SYM	denotes	=
T4416	17238-17240	CD	denotes	15
T4418	17240-17241	:	denotes	;
T4402	17242-17246	NN	denotes	Fig.
T4419	17247-17248	CD	denotes	6
T4420	17248-17249	-RRB-	denotes	)
T4421	17249-17250	.	denotes	.
T4422	17250-17329	sentence	denotes	Annexin A7 deficient platelets showed a slightly lowered aggregation velocity.
T4423	17251-17258	NN	denotes	Annexin
T4424	17259-17261	NN	denotes	A7
T4425	17262-17271	JJ	denotes	deficient
T4426	17272-17281	NNS	denotes	platelets
T4427	17282-17288	VBD	denotes	showed
T4428	17289-17290	DT	denotes	a
T4430	17291-17299	RB	denotes	slightly
T4431	17300-17307	VBN	denotes	lowered
T4432	17308-17319	NN	denotes	aggregation
T4429	17320-17328	NN	denotes	velocity
T4433	17328-17329	.	denotes	.
T4434	17329-17640	sentence	denotes	When we compared the aggregation curves of human platelets from a healthy donor with the ones obtained from an individual with a von Willebrand factor type 1 defect, we found that the difference in the curves was much more pronounced as observed in our studies of healthy mouse platelets and anxA7-/-platelets.
T4435	17330-17334	WRB	denotes	When
T4437	17335-17337	PRP	denotes	we
T4436	17338-17346	VBD	denotes	compared
T4439	17347-17350	DT	denotes	the
T4441	17351-17362	NN	denotes	aggregation
T4440	17363-17369	NNS	denotes	curves
T4442	17370-17372	IN	denotes	of
T4443	17373-17378	JJ	denotes	human
T4444	17379-17388	NNS	denotes	platelets
T4445	17389-17393	IN	denotes	from
T4446	17394-17395	DT	denotes	a
T4448	17396-17403	JJ	denotes	healthy
T4447	17404-17409	NN	denotes	donor
T4449	17410-17414	IN	denotes	with
T4450	17415-17418	DT	denotes	the
T4451	17419-17423	NNS	denotes	ones
T4452	17424-17432	VBN	denotes	obtained
T4453	17433-17437	IN	denotes	from
T4454	17438-17440	DT	denotes	an
T4455	17441-17451	NN	denotes	individual
T4456	17452-17456	IN	denotes	with
T4457	17457-17458	DT	denotes	a
T4459	17459-17462	NN	denotes	von
T4460	17463-17473	NN	denotes	Willebrand
T4461	17474-17480	NN	denotes	factor
T4462	17481-17485	NN	denotes	type
T4463	17486-17487	CD	denotes	1
T4458	17488-17494	NN	denotes	defect
T4464	17494-17496	,	denotes	,
T4465	17496-17498	PRP	denotes	we
T4438	17499-17504	VBD	denotes	found
T4466	17505-17509	IN	denotes	that
T4468	17510-17513	DT	denotes	the
T4469	17514-17524	NN	denotes	difference
T4470	17525-17527	IN	denotes	in
T4471	17528-17531	DT	denotes	the
T4472	17532-17538	NNS	denotes	curves
T4467	17539-17542	VBD	denotes	was
T4473	17543-17547	RB	denotes	much
T4474	17548-17552	RBR	denotes	more
T4475	17553-17563	JJ	denotes	pronounced
T4476	17564-17566	IN	denotes	as
T4477	17567-17575	VBN	denotes	observed
T4478	17576-17578	IN	denotes	in
T4479	17579-17582	PRP$	denotes	our
T4480	17583-17590	NNS	denotes	studies
T4481	17591-17593	IN	denotes	of
T4482	17594-17601	JJ	denotes	healthy
T4484	17602-17607	NN	denotes	mouse
T4483	17608-17617	NNS	denotes	platelets
T4485	17618-17621	CC	denotes	and
T4486	17622-17627	NN	denotes	anxA7
T4488	17627-17628	SYM	denotes	-
T4489	17628-17629	HYPH	denotes	/
T4490	17629-17630	SYM	denotes	-
T4487	17630-17639	NNS	denotes	platelets
T4491	17639-17640	.	denotes	.
T4492	17640-17779	sentence	denotes	Furthermore we found that murine platelets responded immediately to the initiating chemical whereas human platelets have a lag phase [23].
T4493	17641-17652	RB	denotes	Furthermore
T4495	17653-17655	PRP	denotes	we
T4494	17656-17661	VBD	denotes	found
T4496	17662-17666	IN	denotes	that
T4498	17667-17673	JJ	denotes	murine
T4499	17674-17683	NNS	denotes	platelets
T4497	17684-17693	VBD	denotes	responded
T4500	17694-17705	RB	denotes	immediately
T4501	17706-17708	IN	denotes	to
T4502	17709-17712	DT	denotes	the
T4504	17713-17723	VBG	denotes	initiating
T4503	17724-17732	NN	denotes	chemical
T4505	17733-17740	IN	denotes	whereas
T4507	17741-17746	JJ	denotes	human
T4508	17747-17756	NNS	denotes	platelets
T4506	17757-17761	VBP	denotes	have
T4509	17762-17763	DT	denotes	a
T4511	17764-17767	NN	denotes	lag
T4510	17768-17773	NN	denotes	phase
T4512	17774-17775	-LRB-	denotes	[
T4513	17775-17777	CD	denotes	23
T4514	17777-17778	-RRB-	denotes	]
T4515	17778-17779	.	denotes	.
T9783	17790-17798	NN	denotes	Platelet
T9784	17799-17810	NN	denotes	aggregation
T9785	17811-17817	NNS	denotes	curves
T9786	17818-17822	IN	denotes	from
T9787	17823-17831	NN	denotes	platelet
T9788	17832-17836	JJ	denotes	rich
T9789	17837-17843	NN	denotes	plasma
T9790	17844-17846	IN	denotes	of
T9791	17847-17854	NN	denotes	annexin
T9792	17855-17857	NN	denotes	A7
T9794	17858-17863	NN	denotes	knock
T9795	17864-17867	NN	denotes	out
T9796	17868-17871	CC	denotes	and
T9797	17872-17876	JJ	denotes	wild
T9798	17877-17881	NN	denotes	type
T9793	17882-17886	NNS	denotes	mice
T9799	17886-17887	.	denotes	.
T9800	17887-18002	sentence	denotes	The transmission values were measured with an APACT photometer and aggregation was initiated by adding ristocetin.
T9801	17888-17891	DT	denotes	The
T9803	17892-17904	NN	denotes	transmission
T9802	17905-17911	NNS	denotes	values
T9805	17912-17916	VBD	denotes	were
T9804	17917-17925	VBN	denotes	measured
T9806	17926-17930	IN	denotes	with
T9807	17931-17933	DT	denotes	an
T9809	17934-17939	NN	denotes	APACT
T9808	17940-17950	NN	denotes	photometer
T9810	17951-17954	CC	denotes	and
T9811	17955-17966	NN	denotes	aggregation
T9813	17967-17970	VBD	denotes	was
T9812	17971-17980	VBN	denotes	initiated
T9814	17981-17983	IN	denotes	by
T9815	17984-17990	VBG	denotes	adding
T9816	17991-18001	NN	denotes	ristocetin
T9817	18001-18002	.	denotes	.
T9818	18002-18038	sentence	denotes	The first thirty seconds are given.
T9819	18003-18006	DT	denotes	The
T9821	18007-18012	JJ	denotes	first
T9822	18013-18019	CD	denotes	thirty
T9820	18020-18027	NNS	denotes	seconds
T9824	18028-18031	VBP	denotes	are
T9823	18032-18037	VBN	denotes	given
T9825	18037-18038	.	denotes	.
T9826	18038-18101	sentence	denotes	Constant platelet counts were used throughout all experiments.
T9827	18039-18047	JJ	denotes	Constant
T9829	18048-18056	NN	denotes	platelet
T9828	18057-18063	NNS	denotes	counts
T9831	18064-18068	VBD	denotes	were
T9830	18069-18073	VBN	denotes	used
T9832	18074-18084	IN	denotes	throughout
T9833	18085-18088	DT	denotes	all
T9834	18089-18100	NNS	denotes	experiments
T9835	18100-18101	.	denotes	.
T9836	18101-18234	sentence	denotes	The aggregation curve data were analysed for slope values, the maximal aggregation amplitude of every single curve was set to 100 %.
T9837	18102-18105	DT	denotes	The
T9839	18106-18117	NN	denotes	aggregation
T9840	18118-18123	NN	denotes	curve
T9838	18124-18128	NNS	denotes	data
T9842	18129-18133	VBD	denotes	were
T9841	18134-18142	VBN	denotes	analysed
T9844	18143-18146	IN	denotes	for
T9845	18147-18152	NN	denotes	slope
T9846	18153-18159	NNS	denotes	values
T9847	18159-18161	,	denotes	,
T9848	18161-18164	DT	denotes	the
T9850	18165-18172	JJ	denotes	maximal
T9851	18173-18184	NN	denotes	aggregation
T9849	18185-18194	NN	denotes	amplitude
T9852	18195-18197	IN	denotes	of
T9853	18198-18203	DT	denotes	every
T9855	18204-18210	JJ	denotes	single
T9854	18211-18216	NN	denotes	curve
T9856	18217-18220	VBD	denotes	was
T9843	18221-18224	VBN	denotes	set
T9857	18225-18227	IN	denotes	to
T9858	18228-18231	CD	denotes	100
T9859	18232-18233	NN	denotes	%
T9860	18233-18234	.	denotes	.
T9861	18234-18610	sentence	denotes	Both murine curves differ significantly at a time point of seven seconds after platelet initiation, the mutant shows a slightly lower aggregation velocity (p = 0.0287, n knock out = 16, n wild type = 15, mean knock out = 67.7 % transmission, mean wild type = 77.1 % transmission, standard error knock out = 8.3 % transmission, standard error wild type = 13.8 % transmission).
T9862	18235-18239	DT	denotes	Both
T9864	18240-18246	JJ	denotes	murine
T9863	18247-18253	NNS	denotes	curves
T9865	18254-18260	VBP	denotes	differ
T9867	18261-18274	RB	denotes	significantly
T9868	18275-18277	IN	denotes	at
T9869	18278-18279	DT	denotes	a
T9871	18280-18284	NN	denotes	time
T9870	18285-18290	NN	denotes	point
T9872	18291-18293	IN	denotes	of
T9873	18294-18299	CD	denotes	seven
T9874	18300-18307	NNS	denotes	seconds
T9875	18308-18313	IN	denotes	after
T9876	18314-18322	NN	denotes	platelet
T9877	18323-18333	NN	denotes	initiation
T9878	18333-18335	,	denotes	,
T9879	18335-18338	DT	denotes	the
T9880	18339-18345	NN	denotes	mutant
T9866	18346-18351	VBZ	denotes	shows
T9881	18352-18353	DT	denotes	a
T9883	18354-18362	RB	denotes	slightly
T9884	18363-18368	JJR	denotes	lower
T9885	18369-18380	NN	denotes	aggregation
T9882	18381-18389	NN	denotes	velocity
T9886	18390-18391	-LRB-	denotes	(
T9888	18391-18392	NN	denotes	p
T9890	18393-18394	SYM	denotes	=
T9889	18395-18401	CD	denotes	0.0287
T9891	18401-18403	,	denotes	,
T9892	18403-18404	NN	denotes	n
T9894	18405-18410	NN	denotes	knock
T9893	18411-18414	NN	denotes	out
T9896	18415-18416	SYM	denotes	=
T9895	18417-18419	CD	denotes	16
T9897	18419-18421	,	denotes	,
T9898	18421-18422	NN	denotes	n
T9900	18423-18427	JJ	denotes	wild
T9899	18428-18432	NN	denotes	type
T9902	18433-18434	SYM	denotes	=
T9901	18435-18437	CD	denotes	15
T9903	18437-18439	,	denotes	,
T9904	18439-18443	JJ	denotes	mean
T9906	18444-18449	NN	denotes	knock
T9905	18450-18453	NN	denotes	out
T9908	18454-18455	SYM	denotes	=
T9909	18456-18460	CD	denotes	67.7
T9910	18461-18462	NN	denotes	%
T9907	18463-18475	NN	denotes	transmission
T9911	18475-18477	,	denotes	,
T9912	18477-18481	JJ	denotes	mean
T9914	18482-18486	JJ	denotes	wild
T9913	18487-18491	NN	denotes	type
T9916	18492-18493	SYM	denotes	=
T9917	18494-18498	CD	denotes	77.1
T9918	18499-18500	NN	denotes	%
T9915	18501-18513	NN	denotes	transmission
T9919	18513-18515	,	denotes	,
T9920	18515-18523	JJ	denotes	standard
T9921	18524-18529	NN	denotes	error
T9923	18530-18535	NN	denotes	knock
T9922	18536-18539	NN	denotes	out
T9925	18540-18541	SYM	denotes	=
T9926	18542-18545	CD	denotes	8.3
T9927	18546-18547	NN	denotes	%
T9924	18548-18560	NN	denotes	transmission
T9928	18560-18562	,	denotes	,
T9929	18562-18570	JJ	denotes	standard
T9930	18571-18576	NN	denotes	error
T9932	18577-18581	JJ	denotes	wild
T9931	18582-18586	NN	denotes	type
T9933	18587-18588	SYM	denotes	=
T9934	18589-18593	CD	denotes	13.8
T9935	18594-18595	NN	denotes	%
T9887	18596-18608	NN	denotes	transmission
T9936	18608-18609	-RRB-	denotes	)
T9937	18609-18610	.	denotes	.
T9938	18610-18727	sentence	denotes	Murine platelets react immediately to the initiating chemical and show no lag phase like the normal human platelets.
T9939	18611-18617	JJ	denotes	Murine
T9940	18618-18627	NNS	denotes	platelets
T9941	18628-18633	VBP	denotes	react
T9942	18634-18645	RB	denotes	immediately
T9943	18646-18648	IN	denotes	to
T9944	18649-18652	DT	denotes	the
T9946	18653-18663	VBG	denotes	initiating
T9945	18664-18672	NN	denotes	chemical
T9947	18673-18676	CC	denotes	and
T9948	18677-18681	VBP	denotes	show
T9949	18682-18684	DT	denotes	no
T9951	18685-18688	NN	denotes	lag
T9950	18689-18694	NN	denotes	phase
T9952	18695-18699	IN	denotes	like
T9953	18700-18703	DT	denotes	the
T9955	18704-18710	JJ	denotes	normal
T9956	18711-18716	JJ	denotes	human
T9954	18717-18726	NNS	denotes	platelets
T9957	18726-18727	.	denotes	.
T9958	18727-18861	sentence	denotes	For comparison of the range of the platelet impairment slightly affected human platelets are shown as well (von Willebrand syndrome).
T9959	18728-18731	IN	denotes	For
T9961	18732-18742	NN	denotes	comparison
T9962	18743-18745	IN	denotes	of
T9963	18746-18749	DT	denotes	the
T9964	18750-18755	NN	denotes	range
T9965	18756-18758	IN	denotes	of
T9966	18759-18762	DT	denotes	the
T9968	18763-18771	NN	denotes	platelet
T9967	18772-18782	NN	denotes	impairment
T9969	18783-18791	RB	denotes	slightly
T9970	18792-18800	VBN	denotes	affected
T9972	18801-18806	JJ	denotes	human
T9971	18807-18816	NNS	denotes	platelets
T9973	18817-18820	VBP	denotes	are
T9960	18821-18826	VBN	denotes	shown
T9974	18827-18829	RB	denotes	as
T9975	18830-18834	RB	denotes	well
T9976	18835-18836	-LRB-	denotes	(
T9978	18836-18839	NNP	denotes	von
T9979	18840-18850	NNP	denotes	Willebrand
T9977	18851-18859	NN	denotes	syndrome
T9980	18859-18860	-RRB-	denotes	)
T9981	18860-18861	.	denotes	.
T5213	18874-18881	NN	denotes	Annexin
T5214	18882-18884	NN	denotes	A7
T5216	18885-18887	VBZ	denotes	is
T5215	18888-18895	VBN	denotes	thought
T5217	18896-18898	TO	denotes	to
T5218	18899-18903	VB	denotes	have
T5219	18904-18905	DT	denotes	a
T5220	18906-18910	NN	denotes	role
T5221	18911-18913	IN	denotes	in
T5222	18914-18921	NN	denotes	vesicle
T5223	18922-18928	NN	denotes	fusion
T5224	18929-18932	CC	denotes	and
T5225	18933-18935	IN	denotes	in
T5226	18936-18946	VBG	denotes	regulating
T5227	18947-18950	CC	denotes	and
T5228	18951-18962	VBG	denotes	stabilizing
T5229	18963-18971	NN	denotes	membrane
T5230	18972-18979	NNS	denotes	domains
T5231	18980-18981	-LRB-	denotes	[
T5232	18981-18982	CD	denotes	1
T5233	18982-18983	-RRB-	denotes	]
T5234	18983-18984	.	denotes	.
T5235	18984-19164	sentence	denotes	The protein has been localized to the plasma membrane including raft subdomains, furthermore it is present in the cytosol where it is found on subcellular vesicle-like structures.
T5236	18985-18988	DT	denotes	The
T5237	18989-18996	NN	denotes	protein
T5239	18997-19000	VBZ	denotes	has
T5240	19001-19005	VBN	denotes	been
T5238	19006-19015	VBN	denotes	localized
T5242	19016-19018	IN	denotes	to
T5243	19019-19022	DT	denotes	the
T5245	19023-19029	NN	denotes	plasma
T5244	19030-19038	NN	denotes	membrane
T5246	19039-19048	VBG	denotes	including
T5247	19049-19053	NN	denotes	raft
T5248	19054-19064	NNS	denotes	subdomains
T5249	19064-19066	,	denotes	,
T5250	19066-19077	RB	denotes	furthermore
T5251	19078-19080	PRP	denotes	it
T5241	19081-19083	VBZ	denotes	is
T5252	19084-19091	JJ	denotes	present
T5253	19092-19094	IN	denotes	in
T5254	19095-19098	DT	denotes	the
T5255	19099-19106	NN	denotes	cytosol
T5256	19107-19112	WRB	denotes	where
T5258	19113-19115	PRP	denotes	it
T5259	19116-19118	VBZ	denotes	is
T5257	19119-19124	VBN	denotes	found
T5260	19125-19127	IN	denotes	on
T5261	19128-19139	JJ	denotes	subcellular
T5263	19140-19147	NN	denotes	vesicle
T5265	19147-19148	HYPH	denotes	-
T5264	19148-19152	JJ	denotes	like
T5262	19153-19163	NNS	denotes	structures
T5266	19163-19164	.	denotes	.
T5267	19164-19245	sentence	denotes	This distribution appears to be independent of the tissue or cell type analysed.
T5268	19165-19169	DT	denotes	This
T5269	19170-19182	NN	denotes	distribution
T5270	19183-19190	VBZ	denotes	appears
T5271	19191-19193	TO	denotes	to
T5272	19194-19196	VB	denotes	be
T5273	19197-19208	JJ	denotes	independent
T5274	19209-19211	IN	denotes	of
T5275	19212-19215	DT	denotes	the
T5277	19216-19222	NN	denotes	tissue
T5278	19223-19225	CC	denotes	or
T5279	19226-19230	NN	denotes	cell
T5276	19231-19235	NN	denotes	type
T5280	19236-19244	VBN	denotes	analysed
T5281	19244-19245	.	denotes	.
T5282	19245-19399	sentence	denotes	In specialized or terminally differentiated cells, expression levels increase and frequently the larger of the two isoforms is being expressed [6,34,35].
T5283	19246-19248	IN	denotes	In
T5285	19249-19260	VBN	denotes	specialized
T5287	19261-19263	CC	denotes	or
T5288	19264-19274	RB	denotes	terminally
T5289	19275-19289	VBN	denotes	differentiated
T5286	19290-19295	NNS	denotes	cells
T5290	19295-19297	,	denotes	,
T5291	19297-19307	NN	denotes	expression
T5292	19308-19314	NNS	denotes	levels
T5284	19315-19323	VBP	denotes	increase
T5293	19324-19327	CC	denotes	and
T5294	19328-19338	RB	denotes	frequently
T5296	19339-19342	DT	denotes	the
T5297	19343-19349	JJR	denotes	larger
T5298	19350-19352	IN	denotes	of
T5299	19353-19356	DT	denotes	the
T5301	19357-19360	CD	denotes	two
T5300	19361-19369	NNS	denotes	isoforms
T5302	19370-19372	VBZ	denotes	is
T5303	19373-19378	VBG	denotes	being
T5295	19379-19388	VBN	denotes	expressed
T5304	19389-19390	-LRB-	denotes	[
T5306	19390-19391	CD	denotes	6
T5307	19391-19392	,	denotes	,
T5308	19392-19394	CD	denotes	34
T5309	19394-19395	,	denotes	,
T5305	19395-19397	CD	denotes	35
T5310	19397-19398	-RRB-	denotes	]
T5311	19398-19399	.	denotes	.
T5312	19399-19558	sentence	denotes	Red blood cells, which were thought to be devoid of annexin A7, were recently shown to harbor the 47 kDa isoform together with its binding partner sorcin [7].
T5313	19400-19403	JJ	denotes	Red
T5315	19404-19409	NN	denotes	blood
T5314	19410-19415	NNS	denotes	cells
T5317	19415-19417	,	denotes	,
T5318	19417-19422	WDT	denotes	which
T5320	19423-19427	VBD	denotes	were
T5319	19428-19435	VBN	denotes	thought
T5321	19436-19438	TO	denotes	to
T5322	19439-19441	VB	denotes	be
T5323	19442-19448	JJ	denotes	devoid
T5324	19449-19451	IN	denotes	of
T5325	19452-19459	NN	denotes	annexin
T5326	19460-19462	NN	denotes	A7
T5327	19462-19464	,	denotes	,
T5328	19464-19468	VBD	denotes	were
T5329	19469-19477	RB	denotes	recently
T5316	19478-19483	VBN	denotes	shown
T5330	19484-19486	TO	denotes	to
T5331	19487-19493	VB	denotes	harbor
T5332	19494-19497	DT	denotes	the
T5334	19498-19500	CD	denotes	47
T5335	19501-19504	NN	denotes	kDa
T5333	19505-19512	NN	denotes	isoform
T5336	19513-19521	RB	denotes	together
T5337	19522-19526	IN	denotes	with
T5338	19527-19530	PRP$	denotes	its
T5340	19531-19538	VBG	denotes	binding
T5339	19539-19546	NN	denotes	partner
T5341	19547-19553	NN	denotes	sorcin
T5342	19554-19555	-LRB-	denotes	[
T5343	19555-19556	CD	denotes	7
T5344	19556-19557	-RRB-	denotes	]
T5345	19557-19558	.	denotes	.
T5346	19558-19682	sentence	denotes	We showed the presence of the 51 kDa isoform as well, adding red blood cells to the list of cells that harbor this isoform.
T5347	19559-19561	PRP	denotes	We
T5348	19562-19568	VBD	denotes	showed
T5349	19569-19572	DT	denotes	the
T5350	19573-19581	NN	denotes	presence
T5351	19582-19584	IN	denotes	of
T5352	19585-19588	DT	denotes	the
T5354	19589-19591	CD	denotes	51
T5355	19592-19595	NN	denotes	kDa
T5353	19596-19603	NN	denotes	isoform
T5356	19604-19606	RB	denotes	as
T5357	19607-19611	RB	denotes	well
T5358	19611-19613	,	denotes	,
T5359	19613-19619	VBG	denotes	adding
T5360	19620-19623	JJ	denotes	red
T5362	19624-19629	NN	denotes	blood
T5361	19630-19635	NNS	denotes	cells
T5363	19636-19638	IN	denotes	to
T5364	19639-19642	DT	denotes	the
T5365	19643-19647	NN	denotes	list
T5366	19648-19650	IN	denotes	of
T5367	19651-19656	NNS	denotes	cells
T5368	19657-19661	WDT	denotes	that
T5369	19662-19668	VBP	denotes	harbor
T5370	19669-19673	DT	denotes	this
T5371	19674-19681	NN	denotes	isoform
T5372	19681-19682	.	denotes	.
T5373	19682-19906	sentence	denotes	Salzer et al. [7] not only demonstrated the presence of annexin A7 in red blood cells, they also showed that annexin A7 and sorcin were enriched in membrane raft domains of nanovesicles formed from red blood cells in vitro.
T5374	19683-19689	NNP	denotes	Salzer
T5376	19690-19692	FW	denotes	et
T5377	19693-19696	FW	denotes	al.
T5378	19697-19698	-LRB-	denotes	[
T5379	19698-19699	CD	denotes	7
T5380	19699-19700	-RRB-	denotes	]
T5381	19701-19704	RB	denotes	not
T5382	19705-19709	RB	denotes	only
T5383	19710-19722	VBD	denotes	demonstrated
T5384	19723-19726	DT	denotes	the
T5385	19727-19735	NN	denotes	presence
T5386	19736-19738	IN	denotes	of
T5387	19739-19746	NN	denotes	annexin
T5388	19747-19749	NN	denotes	A7
T5389	19750-19752	IN	denotes	in
T5390	19753-19756	JJ	denotes	red
T5392	19757-19762	NN	denotes	blood
T5391	19763-19768	NNS	denotes	cells
T5393	19768-19770	,	denotes	,
T5394	19770-19774	PRP	denotes	they
T5395	19775-19779	RB	denotes	also
T5375	19780-19786	VBD	denotes	showed
T5396	19787-19791	IN	denotes	that
T5398	19792-19799	NN	denotes	annexin
T5399	19800-19802	NN	denotes	A7
T5400	19803-19806	CC	denotes	and
T5401	19807-19813	NN	denotes	sorcin
T5397	19814-19818	VBD	denotes	were
T5402	19819-19827	JJ	denotes	enriched
T5403	19828-19830	IN	denotes	in
T5404	19831-19839	NN	denotes	membrane
T5405	19840-19844	NN	denotes	raft
T5406	19845-19852	NNS	denotes	domains
T5407	19853-19855	IN	denotes	of
T5408	19856-19868	NNS	denotes	nanovesicles
T5409	19869-19875	VBN	denotes	formed
T5410	19876-19880	IN	denotes	from
T5411	19881-19884	JJ	denotes	red
T5413	19885-19890	NN	denotes	blood
T5412	19891-19896	NNS	denotes	cells
T5414	19897-19899	FW	denotes	in
T5415	19900-19905	FW	denotes	vitro
T5416	19905-19906	.	denotes	.
T5417	19906-20025	sentence	denotes	Raft domains in general are considered key players in membrane organization and in mediating the vesiculation process.
T5418	19907-19911	NN	denotes	Raft
T5419	19912-19919	NNS	denotes	domains
T5421	19920-19922	IN	denotes	in
T5422	19923-19930	JJ	denotes	general
T5423	19931-19934	VBP	denotes	are
T5420	19935-19945	VBN	denotes	considered
T5424	19946-19949	JJ	denotes	key
T5425	19950-19957	NNS	denotes	players
T5426	19958-19960	IN	denotes	in
T5427	19961-19969	NN	denotes	membrane
T5428	19970-19982	NN	denotes	organization
T5429	19983-19986	CC	denotes	and
T5430	19987-19989	IN	denotes	in
T5431	19990-19999	VBG	denotes	mediating
T5432	20000-20003	DT	denotes	the
T5434	20004-20016	NN	denotes	vesiculation
T5433	20017-20024	NN	denotes	process
T5435	20024-20025	.	denotes	.
T5436	20025-20229	sentence	denotes	They result from the clustering of sphingolipids and cholesterol in the outer leaflet of the membrane connected to phospholipids and cholesterol in the inner leaflet and are enriched in special proteins.
T5437	20026-20030	PRP	denotes	They
T5438	20031-20037	VBP	denotes	result
T5439	20038-20042	IN	denotes	from
T5440	20043-20046	DT	denotes	the
T5441	20047-20057	NN	denotes	clustering
T5442	20058-20060	IN	denotes	of
T5443	20061-20074	NNS	denotes	sphingolipids
T5444	20075-20078	CC	denotes	and
T5445	20079-20090	NN	denotes	cholesterol
T5446	20091-20093	IN	denotes	in
T5447	20094-20097	DT	denotes	the
T5449	20098-20103	JJ	denotes	outer
T5448	20104-20111	NN	denotes	leaflet
T5450	20112-20114	IN	denotes	of
T5451	20115-20118	DT	denotes	the
T5452	20119-20127	NN	denotes	membrane
T5453	20128-20137	VBN	denotes	connected
T5454	20138-20140	IN	denotes	to
T5455	20141-20154	NNS	denotes	phospholipids
T5456	20155-20158	CC	denotes	and
T5457	20159-20170	NN	denotes	cholesterol
T5458	20171-20173	IN	denotes	in
T5459	20174-20177	DT	denotes	the
T5461	20178-20183	JJ	denotes	inner
T5460	20184-20191	NN	denotes	leaflet
T5462	20192-20195	CC	denotes	and
T5463	20196-20199	VBP	denotes	are
T5464	20200-20208	JJ	denotes	enriched
T5465	20209-20211	IN	denotes	in
T5466	20212-20219	JJ	denotes	special
T5467	20220-20228	NN	denotes	proteins
T5468	20228-20229	.	denotes	.
T5469	20229-20405	sentence	denotes	Biochemically rafts are defined as membranes that are resistant to extraction by cold Triton X-100 and can be floated to low densities in sucrose gradient centrifugation [36].
T5470	20230-20243	RB	denotes	Biochemically
T5472	20244-20249	NNS	denotes	rafts
T5473	20250-20253	VBP	denotes	are
T5471	20254-20261	VBN	denotes	defined
T5474	20262-20264	IN	denotes	as
T5475	20265-20274	NNS	denotes	membranes
T5476	20275-20279	WDT	denotes	that
T5477	20280-20283	VBP	denotes	are
T5478	20284-20293	JJ	denotes	resistant
T5479	20294-20296	IN	denotes	to
T5480	20297-20307	NN	denotes	extraction
T5481	20308-20310	IN	denotes	by
T5482	20311-20315	JJ	denotes	cold
T5484	20316-20322	NN	denotes	Triton
T5483	20323-20324	NN	denotes	X
T5485	20324-20325	HYPH	denotes	-
T5486	20325-20328	CD	denotes	100
T5487	20329-20332	CC	denotes	and
T5488	20333-20336	MD	denotes	can
T5490	20337-20339	VB	denotes	be
T5489	20340-20347	VBN	denotes	floated
T5491	20348-20350	IN	denotes	to
T5492	20351-20354	JJ	denotes	low
T5493	20355-20364	NNS	denotes	densities
T5494	20365-20367	IN	denotes	in
T5495	20368-20375	NN	denotes	sucrose
T5496	20376-20384	NN	denotes	gradient
T5497	20385-20399	NN	denotes	centrifugation
T5498	20400-20401	-LRB-	denotes	[
T5499	20401-20403	CD	denotes	36
T5500	20403-20404	-RRB-	denotes	]
T5501	20404-20405	.	denotes	.
T5502	20405-20564	sentence	denotes	The findings of Caohuy et al. [37] that annexin A7 mediates membrane aggregation also suggested that annexin A7 is an essential component during vesiculation.
T5503	20406-20409	DT	denotes	The
T5504	20410-20418	NNS	denotes	findings
T5506	20419-20421	IN	denotes	of
T5507	20422-20428	NNP	denotes	Caohuy
T5508	20429-20431	FW	denotes	et
T5509	20432-20435	FW	denotes	al.
T5510	20436-20437	-LRB-	denotes	[
T5511	20437-20439	CD	denotes	37
T5512	20439-20440	-RRB-	denotes	]
T5513	20441-20445	IN	denotes	that
T5515	20446-20453	NN	denotes	annexin
T5516	20454-20456	NN	denotes	A7
T5514	20457-20465	VBZ	denotes	mediates
T5517	20466-20474	NN	denotes	membrane
T5518	20475-20486	NN	denotes	aggregation
T5519	20487-20491	RB	denotes	also
T5505	20492-20501	VBD	denotes	suggested
T5520	20502-20506	IN	denotes	that
T5522	20507-20514	NN	denotes	annexin
T5523	20515-20517	NN	denotes	A7
T5521	20518-20520	VBZ	denotes	is
T5524	20521-20523	DT	denotes	an
T5526	20524-20533	JJ	denotes	essential
T5525	20534-20543	NN	denotes	component
T5527	20544-20550	IN	denotes	during
T5528	20551-20563	NN	denotes	vesiculation
T5529	20563-20564	.	denotes	.
T5530	20564-20693	sentence	denotes	In addition they reported a further activation of the vesiculation process by GTP and proposed that annexin A7 acts as a GTPase.
T5531	20565-20567	IN	denotes	In
T5533	20568-20576	NN	denotes	addition
T5534	20577-20581	PRP	denotes	they
T5532	20582-20590	VBD	denotes	reported
T5535	20591-20592	DT	denotes	a
T5537	20593-20600	JJ	denotes	further
T5536	20601-20611	NN	denotes	activation
T5538	20612-20614	IN	denotes	of
T5539	20615-20618	DT	denotes	the
T5541	20619-20631	NN	denotes	vesiculation
T5540	20632-20639	NN	denotes	process
T5542	20640-20642	IN	denotes	by
T5543	20643-20646	NN	denotes	GTP
T5544	20647-20650	CC	denotes	and
T5545	20651-20659	VBD	denotes	proposed
T5546	20660-20664	IN	denotes	that
T5548	20665-20672	NN	denotes	annexin
T5549	20673-20675	NN	denotes	A7
T5547	20676-20680	VBZ	denotes	acts
T5550	20681-20683	IN	denotes	as
T5551	20684-20685	DT	denotes	a
T5552	20686-20692	NN	denotes	GTPase
T5553	20692-20693	.	denotes	.
T5554	20693-20776	sentence	denotes	Similarly, the presence of GTP enhanced the secretion of surfactant by annexin A7.
T5555	20694-20703	RB	denotes	Similarly
T5557	20703-20705	,	denotes	,
T5558	20705-20708	DT	denotes	the
T5559	20709-20717	NN	denotes	presence
T5560	20718-20720	IN	denotes	of
T5561	20721-20724	NN	denotes	GTP
T5556	20725-20733	VBD	denotes	enhanced
T5562	20734-20737	DT	denotes	the
T5563	20738-20747	NN	denotes	secretion
T5564	20748-20750	IN	denotes	of
T5565	20751-20761	NN	denotes	surfactant
T5566	20762-20764	IN	denotes	by
T5567	20765-20772	NN	denotes	annexin
T5568	20773-20775	NN	denotes	A7
T5569	20775-20776	.	denotes	.
T5570	20776-20983	sentence	denotes	These results were thought to support a direct role for annexin A7 in surfactant secretion, but in these studies annexin A7 mediated membrane fusion was separated from a second GTP-dependent mechanism [38].
T5571	20777-20782	DT	denotes	These
T5572	20783-20790	NNS	denotes	results
T5574	20791-20795	VBD	denotes	were
T5573	20796-20803	VBN	denotes	thought
T5575	20804-20806	TO	denotes	to
T5576	20807-20814	VB	denotes	support
T5577	20815-20816	DT	denotes	a
T5579	20817-20823	JJ	denotes	direct
T5578	20824-20828	NN	denotes	role
T5580	20829-20832	IN	denotes	for
T5581	20833-20840	NN	denotes	annexin
T5582	20841-20843	NN	denotes	A7
T5583	20844-20846	IN	denotes	in
T5584	20847-20857	JJ	denotes	surfactant
T5585	20858-20867	NN	denotes	secretion
T5586	20867-20869	,	denotes	,
T5587	20869-20872	CC	denotes	but
T5588	20873-20875	IN	denotes	in
T5590	20876-20881	DT	denotes	these
T5591	20882-20889	NNS	denotes	studies
T5592	20890-20897	NN	denotes	annexin
T5593	20898-20900	NN	denotes	A7
T5594	20901-20909	VBN	denotes	mediated
T5596	20910-20918	NN	denotes	membrane
T5595	20919-20925	NN	denotes	fusion
T5597	20926-20929	VBD	denotes	was
T5589	20930-20939	VBN	denotes	separated
T5598	20940-20944	IN	denotes	from
T5599	20945-20946	DT	denotes	a
T5601	20947-20953	JJ	denotes	second
T5602	20954-20957	NN	denotes	GTP
T5604	20957-20958	HYPH	denotes	-
T5603	20958-20967	JJ	denotes	dependent
T5600	20968-20977	NN	denotes	mechanism
T5605	20978-20979	-LRB-	denotes	[
T5606	20979-20981	CD	denotes	38
T5607	20981-20982	-RRB-	denotes	]
T5608	20982-20983	.	denotes	.
T5609	20983-21098	sentence	denotes	Having an annexin A7 knockout mouse we tested the involvement of annexin A7 in budding of red blood cell vesicles.
T5610	20984-20990	VBG	denotes	Having
T5612	20991-20993	DT	denotes	an
T5614	20994-21001	NN	denotes	annexin
T5615	21002-21004	NN	denotes	A7
T5616	21005-21013	NN	denotes	knockout
T5613	21014-21019	NN	denotes	mouse
T5617	21020-21022	PRP	denotes	we
T5611	21023-21029	VBD	denotes	tested
T5618	21030-21033	DT	denotes	the
T5619	21034-21045	NN	denotes	involvement
T5620	21046-21048	IN	denotes	of
T5621	21049-21056	NN	denotes	annexin
T5622	21057-21059	NN	denotes	A7
T5623	21060-21062	IN	denotes	in
T5624	21063-21070	NN	denotes	budding
T5625	21071-21073	IN	denotes	of
T5626	21074-21077	JJ	denotes	red
T5628	21078-21083	NN	denotes	blood
T5627	21084-21088	NN	denotes	cell
T5629	21089-21097	NNS	denotes	vesicles
T5630	21097-21098	.	denotes	.
T5631	21098-21243	sentence	denotes	Our data suggest that the efficiency of micro- and nanovesicle formation is not significantly different in wild type and mutant red blood cells.
T5632	21099-21102	PRP$	denotes	Our
T5633	21103-21107	NNS	denotes	data
T5634	21108-21115	VBP	denotes	suggest
T5635	21116-21120	IN	denotes	that
T5637	21121-21124	DT	denotes	the
T5638	21125-21135	NN	denotes	efficiency
T5639	21136-21138	IN	denotes	of
T5640	21139-21144	AFX	denotes	micro
T5642	21144-21145	HYPH	denotes	-
T5643	21146-21149	CC	denotes	and
T5641	21150-21161	NN	denotes	nanovesicle
T5644	21162-21171	NN	denotes	formation
T5636	21172-21174	VBZ	denotes	is
T5645	21175-21178	RB	denotes	not
T5646	21179-21192	RB	denotes	significantly
T5647	21193-21202	JJ	denotes	different
T5648	21203-21205	IN	denotes	in
T5649	21206-21210	JJ	denotes	wild
T5650	21211-21215	NN	denotes	type
T5652	21216-21219	CC	denotes	and
T5653	21220-21226	JJ	denotes	mutant
T5654	21227-21230	JJ	denotes	red
T5655	21231-21236	NN	denotes	blood
T5651	21237-21242	NNS	denotes	cells
T5656	21242-21243	.	denotes	.
T5657	21243-21422	sentence	denotes	Likewise, Dictyostelium discoideum cells, in which the single annexin gene was inactivated, were not impaired in processes requiring membrane fusion [[39]; and data unpublished].
T5658	21244-21252	RB	denotes	Likewise
T5660	21252-21254	,	denotes	,
T5661	21254-21267	NNP	denotes	Dictyostelium
T5662	21268-21278	NNP	denotes	discoideum
T5663	21279-21284	NNS	denotes	cells
T5664	21284-21286	,	denotes	,
T5665	21286-21288	IN	denotes	in
T5667	21289-21294	WDT	denotes	which
T5668	21295-21298	DT	denotes	the
T5670	21299-21305	JJ	denotes	single
T5671	21306-21313	NN	denotes	annexin
T5669	21314-21318	NN	denotes	gene
T5666	21319-21322	VBD	denotes	was
T5672	21323-21334	JJ	denotes	inactivated
T5673	21334-21336	,	denotes	,
T5659	21336-21340	VBD	denotes	were
T5674	21341-21344	RB	denotes	not
T5675	21345-21353	JJ	denotes	impaired
T5676	21354-21356	IN	denotes	in
T5677	21357-21366	NNS	denotes	processes
T5678	21367-21376	VBG	denotes	requiring
T5679	21377-21385	NN	denotes	membrane
T5680	21386-21392	NN	denotes	fusion
T5681	21393-21394	-LRB-	denotes	[
T5683	21394-21395	-LRB-	denotes	[
T5682	21395-21397	CD	denotes	39
T5684	21397-21398	-RRB-	denotes	]
T5685	21398-21399	:	denotes	;
T5686	21400-21403	CC	denotes	and
T5687	21404-21408	NNS	denotes	data
T5688	21409-21420	JJ	denotes	unpublished
T5689	21420-21421	-RRB-	denotes	]
T5690	21421-21422	.	denotes	.
T5691	21422-21584	sentence	denotes	It might well be that the observed fusion of phospholipid layers by annexin A7 in the presence of high Ca2+ concentrations may turn out to be an in vitro effect.
T5692	21423-21425	PRP	denotes	It
T5694	21426-21431	MD	denotes	might
T5695	21432-21436	RB	denotes	well
T5693	21437-21439	VB	denotes	be
T5696	21440-21444	IN	denotes	that
T5698	21445-21448	DT	denotes	the
T5700	21449-21457	VBN	denotes	observed
T5699	21458-21464	NN	denotes	fusion
T5701	21465-21467	IN	denotes	of
T5702	21468-21480	NN	denotes	phospholipid
T5703	21481-21487	NNS	denotes	layers
T5704	21488-21490	IN	denotes	by
T5705	21491-21498	NN	denotes	annexin
T5706	21499-21501	NN	denotes	A7
T5707	21502-21504	IN	denotes	in
T5708	21505-21508	DT	denotes	the
T5709	21509-21517	NN	denotes	presence
T5710	21518-21520	IN	denotes	of
T5711	21521-21525	JJ	denotes	high
T5713	21526-21530	NN	denotes	Ca2+
T5712	21531-21545	NNS	denotes	concentrations
T5714	21546-21549	MD	denotes	may
T5697	21550-21554	VB	denotes	turn
T5715	21555-21558	RP	denotes	out
T5716	21559-21561	TO	denotes	to
T5717	21562-21564	VB	denotes	be
T5718	21565-21567	DT	denotes	an
T5720	21568-21570	FW	denotes	in
T5721	21571-21576	FW	denotes	vitro
T5719	21577-21583	NN	denotes	effect
T5722	21583-21584	.	denotes	.
T5723	21584-21851	sentence	denotes	Similarly, the high abundance of annexin A7 in micro- and nanovesicles might be fortuitous as a significant percentage of the red blood cell membrane, with which annexin A7 associates when the Ca2+ levels rise, appears to be involved in the formation of exovesicles.
T5724	21585-21594	RB	denotes	Similarly
T5726	21594-21596	,	denotes	,
T5727	21596-21599	DT	denotes	the
T5729	21600-21604	JJ	denotes	high
T5728	21605-21614	NN	denotes	abundance
T5730	21615-21617	IN	denotes	of
T5731	21618-21625	NN	denotes	annexin
T5732	21626-21628	NN	denotes	A7
T5733	21629-21631	IN	denotes	in
T5734	21632-21637	AFX	denotes	micro
T5736	21637-21638	HYPH	denotes	-
T5737	21639-21642	CC	denotes	and
T5735	21643-21655	NNS	denotes	nanovesicles
T5738	21656-21661	MD	denotes	might
T5725	21662-21664	VB	denotes	be
T5739	21665-21675	JJ	denotes	fortuitous
T5740	21676-21678	IN	denotes	as
T5742	21679-21680	DT	denotes	a
T5744	21681-21692	JJ	denotes	significant
T5743	21693-21703	NN	denotes	percentage
T5745	21704-21706	IN	denotes	of
T5746	21707-21710	DT	denotes	the
T5748	21711-21714	JJ	denotes	red
T5750	21715-21720	NN	denotes	blood
T5749	21721-21725	NN	denotes	cell
T5747	21726-21734	NN	denotes	membrane
T5751	21734-21736	,	denotes	,
T5752	21736-21740	IN	denotes	with
T5754	21741-21746	WDT	denotes	which
T5755	21747-21754	NN	denotes	annexin
T5756	21755-21757	NN	denotes	A7
T5753	21758-21768	VBZ	denotes	associates
T5757	21769-21773	WRB	denotes	when
T5759	21774-21777	DT	denotes	the
T5761	21778-21782	NN	denotes	Ca2+
T5760	21783-21789	NNS	denotes	levels
T5758	21790-21794	VBP	denotes	rise
T5762	21794-21796	,	denotes	,
T5741	21796-21803	VBZ	denotes	appears
T5763	21804-21806	TO	denotes	to
T5765	21807-21809	VB	denotes	be
T5764	21810-21818	VBN	denotes	involved
T5766	21819-21821	IN	denotes	in
T5767	21822-21825	DT	denotes	the
T5768	21826-21835	NN	denotes	formation
T5769	21836-21838	IN	denotes	of
T5770	21839-21850	NNS	denotes	exovesicles
T5771	21850-21851	.	denotes	.
T5772	21851-22036	sentence	denotes	Furthermore, the distribution of both annexin A7 isoforms in gradients of red blood cells indicates a function which differs from organizing subcellular membranes or membrane pathways.
T5773	21852-21863	RB	denotes	Furthermore
T5775	21863-21865	,	denotes	,
T5776	21865-21868	DT	denotes	the
T5777	21869-21881	NN	denotes	distribution
T5778	21882-21884	IN	denotes	of
T5779	21885-21889	DT	denotes	both
T5781	21890-21897	NN	denotes	annexin
T5782	21898-21900	NN	denotes	A7
T5780	21901-21909	NNS	denotes	isoforms
T5783	21910-21912	IN	denotes	in
T5784	21913-21922	NNS	denotes	gradients
T5785	21923-21925	IN	denotes	of
T5786	21926-21929	JJ	denotes	red
T5788	21930-21935	NN	denotes	blood
T5787	21936-21941	NNS	denotes	cells
T5774	21942-21951	VBZ	denotes	indicates
T5789	21952-21953	DT	denotes	a
T5790	21954-21962	NN	denotes	function
T5791	21963-21968	WDT	denotes	which
T5792	21969-21976	VBZ	denotes	differs
T5793	21977-21981	IN	denotes	from
T5794	21982-21992	VBG	denotes	organizing
T5795	21993-22004	JJ	denotes	subcellular
T5796	22005-22014	NNS	denotes	membranes
T5797	22015-22017	CC	denotes	or
T5798	22018-22026	NN	denotes	membrane
T5799	22027-22035	NNS	denotes	pathways
T5800	22035-22036	.	denotes	.
T5801	22036-22252	sentence	denotes	Whereas annexin A2 is tightly associated with endosomes and may have functions in membrane fusion and the endosomal pathway, annexin A7 distributes with plasma membrane raft domains of different types and densities.
T5802	22037-22044	IN	denotes	Whereas
T5804	22045-22052	NN	denotes	annexin
T5805	22053-22055	NN	denotes	A2
T5806	22056-22058	VBZ	denotes	is
T5807	22059-22066	RB	denotes	tightly
T5803	22067-22077	VBN	denotes	associated
T5809	22078-22082	IN	denotes	with
T5810	22083-22092	NNS	denotes	endosomes
T5811	22093-22096	CC	denotes	and
T5812	22097-22100	MD	denotes	may
T5813	22101-22105	VB	denotes	have
T5814	22106-22115	NNS	denotes	functions
T5815	22116-22118	IN	denotes	in
T5816	22119-22127	NN	denotes	membrane
T5817	22128-22134	NN	denotes	fusion
T5818	22135-22138	CC	denotes	and
T5819	22139-22142	DT	denotes	the
T5821	22143-22152	JJ	denotes	endosomal
T5820	22153-22160	NN	denotes	pathway
T5822	22160-22162	,	denotes	,
T5823	22162-22169	NN	denotes	annexin
T5824	22170-22172	NN	denotes	A7
T5808	22173-22184	VBZ	denotes	distributes
T5825	22185-22189	IN	denotes	with
T5826	22190-22196	NN	denotes	plasma
T5827	22197-22205	NN	denotes	membrane
T5829	22206-22210	NN	denotes	raft
T5828	22211-22218	NNS	denotes	domains
T5830	22219-22221	IN	denotes	of
T5831	22222-22231	JJ	denotes	different
T5832	22232-22237	NNS	denotes	types
T5833	22238-22241	CC	denotes	and
T5834	22242-22251	NNS	denotes	densities
T5835	22251-22252	.	denotes	.
T5836	22252-22486	sentence	denotes	Interestingly, in vesicles of red blood cells lacking annexin A7 the amount of sorcin is lowered, indicating that its localisation is affected by the loss of annexin A7 or its expression during red blood cell development was reduced.
T5837	22253-22266	RB	denotes	Interestingly
T5839	22266-22268	,	denotes	,
T5840	22268-22270	IN	denotes	in
T5841	22271-22279	NNS	denotes	vesicles
T5842	22280-22282	IN	denotes	of
T5843	22283-22286	JJ	denotes	red
T5845	22287-22292	NN	denotes	blood
T5844	22293-22298	NNS	denotes	cells
T5846	22299-22306	VBG	denotes	lacking
T5847	22307-22314	NN	denotes	annexin
T5848	22315-22317	NN	denotes	A7
T5849	22318-22321	DT	denotes	the
T5850	22322-22328	NN	denotes	amount
T5851	22329-22331	IN	denotes	of
T5852	22332-22338	NN	denotes	sorcin
T5853	22339-22341	VBZ	denotes	is
T5838	22342-22349	VBN	denotes	lowered
T5854	22349-22351	,	denotes	,
T5855	22351-22361	VBG	denotes	indicating
T5856	22362-22366	IN	denotes	that
T5858	22367-22370	PRP$	denotes	its
T5859	22371-22383	NN	denotes	localisation
T5860	22384-22386	VBZ	denotes	is
T5857	22387-22395	VBN	denotes	affected
T5861	22396-22398	IN	denotes	by
T5862	22399-22402	DT	denotes	the
T5863	22403-22407	NN	denotes	loss
T5864	22408-22410	IN	denotes	of
T5865	22411-22418	NN	denotes	annexin
T5866	22419-22421	NN	denotes	A7
T5867	22422-22424	CC	denotes	or
T5868	22425-22428	PRP$	denotes	its
T5869	22429-22439	NN	denotes	expression
T5871	22440-22446	IN	denotes	during
T5872	22447-22450	JJ	denotes	red
T5874	22451-22456	NN	denotes	blood
T5873	22457-22461	NN	denotes	cell
T5875	22462-22473	NN	denotes	development
T5876	22474-22477	VBD	denotes	was
T5870	22478-22485	VBN	denotes	reduced
T5877	22485-22486	.	denotes	.
T5878	22486-22625	sentence	denotes	Having excluded a direct role in vesicle formation and membrane fusion, annexin A7 might act by its property as Ca2+-binding protein [17].
T5879	22487-22493	VBG	denotes	Having
T5880	22494-22502	VBN	denotes	excluded
T5882	22503-22504	DT	denotes	a
T5884	22505-22511	JJ	denotes	direct
T5883	22512-22516	NN	denotes	role
T5885	22517-22519	IN	denotes	in
T5886	22520-22527	NN	denotes	vesicle
T5887	22528-22537	NN	denotes	formation
T5888	22538-22541	CC	denotes	and
T5889	22542-22550	NN	denotes	membrane
T5890	22551-22557	NN	denotes	fusion
T5891	22557-22559	,	denotes	,
T5892	22559-22566	NN	denotes	annexin
T5893	22567-22569	NN	denotes	A7
T5894	22570-22575	MD	denotes	might
T5881	22576-22579	VB	denotes	act
T5895	22580-22582	IN	denotes	by
T5896	22583-22586	PRP$	denotes	its
T5897	22587-22595	NN	denotes	property
T5898	22596-22598	IN	denotes	as
T5899	22599-22603	NN	denotes	Ca2+
T5901	22603-22604	HYPH	denotes	-
T5900	22604-22611	VBG	denotes	binding
T5902	22612-22619	NN	denotes	protein
T5903	22620-22621	-LRB-	denotes	[
T5904	22621-22623	CD	denotes	17
T5905	22623-22624	-RRB-	denotes	]
T5906	22624-22625	.	denotes	.
T5907	22625-22863	sentence	denotes	Previous results in which annexin A7 was shown to play a role in membrane aggregation might be explained by a supportive function of annexin A7 in that it interferes with the local Ca2+ homeostasis thus influencing membrane organization.
T5908	22626-22634	JJ	denotes	Previous
T5909	22635-22642	NNS	denotes	results
T5911	22643-22645	IN	denotes	in
T5913	22646-22651	WDT	denotes	which
T5914	22652-22659	NN	denotes	annexin
T5915	22660-22662	NN	denotes	A7
T5916	22663-22666	VBD	denotes	was
T5912	22667-22672	VBN	denotes	shown
T5917	22673-22675	TO	denotes	to
T5918	22676-22680	VB	denotes	play
T5919	22681-22682	DT	denotes	a
T5920	22683-22687	NN	denotes	role
T5921	22688-22690	IN	denotes	in
T5922	22691-22699	NN	denotes	membrane
T5923	22700-22711	NN	denotes	aggregation
T5924	22712-22717	MD	denotes	might
T5925	22718-22720	VB	denotes	be
T5910	22721-22730	VBN	denotes	explained
T5926	22731-22733	IN	denotes	by
T5927	22734-22735	DT	denotes	a
T5929	22736-22746	JJ	denotes	supportive
T5928	22747-22755	NN	denotes	function
T5930	22756-22758	IN	denotes	of
T5931	22759-22766	NN	denotes	annexin
T5932	22767-22769	NN	denotes	A7
T5933	22770-22772	IN	denotes	in
T5935	22773-22777	IN	denotes	that
T5936	22778-22780	PRP	denotes	it
T5934	22781-22791	VBZ	denotes	interferes
T5937	22792-22796	IN	denotes	with
T5938	22797-22800	DT	denotes	the
T5940	22801-22806	JJ	denotes	local
T5941	22807-22811	NN	denotes	Ca2+
T5939	22812-22823	NN	denotes	homeostasis
T5942	22824-22828	RB	denotes	thus
T5943	22829-22840	VBG	denotes	influencing
T5944	22841-22849	NN	denotes	membrane
T5945	22850-22862	NN	denotes	organization
T5946	22862-22863	.	denotes	.
T5947	22863-23001	sentence	denotes	As the annexins belong to a wide spread and evolutionarily conserved protein family, redundant, but not identical functions are expected.
T5948	22864-22866	IN	denotes	As
T5950	22867-22870	DT	denotes	the
T5951	22871-22879	NNS	denotes	annexins
T5949	22880-22886	VBP	denotes	belong
T5953	22887-22889	IN	denotes	to
T5954	22890-22891	DT	denotes	a
T5956	22892-22896	RB	denotes	wide
T5957	22897-22903	VBN	denotes	spread
T5958	22904-22907	CC	denotes	and
T5959	22908-22922	RB	denotes	evolutionarily
T5960	22923-22932	VBN	denotes	conserved
T5961	22933-22940	NN	denotes	protein
T5955	22941-22947	NN	denotes	family
T5962	22947-22949	,	denotes	,
T5963	22949-22958	NN	denotes	redundant
T5965	22958-22960	,	denotes	,
T5966	22960-22963	CC	denotes	but
T5967	22964-22967	RB	denotes	not
T5964	22968-22977	JJ	denotes	identical
T5968	22978-22987	NNS	denotes	functions
T5969	22988-22991	VBP	denotes	are
T5952	22992-23000	VBN	denotes	expected
T5970	23000-23001	.	denotes	.
T5971	23001-23134	sentence	denotes	However, so far no other members of the annexin family, which may substitute for annexin A7, have been described in red blood cells.
T5972	23002-23009	RB	denotes	However
T5974	23009-23011	,	denotes	,
T5975	23011-23013	RB	denotes	so
T5976	23014-23017	RB	denotes	far
T5977	23018-23020	DT	denotes	no
T5979	23021-23026	JJ	denotes	other
T5978	23027-23034	NNS	denotes	members
T5980	23035-23037	IN	denotes	of
T5981	23038-23041	DT	denotes	the
T5983	23042-23049	NN	denotes	annexin
T5982	23050-23056	NN	denotes	family
T5984	23056-23058	,	denotes	,
T5985	23058-23063	WDT	denotes	which
T5987	23064-23067	MD	denotes	may
T5986	23068-23078	VB	denotes	substitute
T5988	23079-23082	IN	denotes	for
T5989	23083-23090	NN	denotes	annexin
T5990	23091-23093	NN	denotes	A7
T5991	23093-23095	,	denotes	,
T5992	23095-23099	VBP	denotes	have
T5993	23100-23104	VBN	denotes	been
T5973	23105-23114	VBN	denotes	described
T5994	23115-23117	IN	denotes	in
T5995	23118-23121	JJ	denotes	red
T5997	23122-23127	NN	denotes	blood
T5996	23128-23133	NNS	denotes	cells
T5998	23133-23134	.	denotes	.
T5999	23134-23326	sentence	denotes	The structural basis for the elasticy of red blood cells in circulation are long range molecular functions of the plasma membrane and the membrane associated part of the cytoskeleton [40-43].
T6000	23135-23138	DT	denotes	The
T6002	23139-23149	JJ	denotes	structural
T6001	23150-23155	NN	denotes	basis
T6004	23156-23159	IN	denotes	for
T6005	23160-23163	DT	denotes	the
T6006	23164-23172	NN	denotes	elasticy
T6007	23173-23175	IN	denotes	of
T6008	23176-23179	JJ	denotes	red
T6010	23180-23185	NN	denotes	blood
T6009	23186-23191	NNS	denotes	cells
T6011	23192-23194	IN	denotes	in
T6012	23195-23206	NN	denotes	circulation
T6003	23207-23210	VBP	denotes	are
T6013	23211-23215	JJ	denotes	long
T6014	23216-23221	NN	denotes	range
T6016	23222-23231	JJ	denotes	molecular
T6015	23232-23241	NNS	denotes	functions
T6017	23242-23244	IN	denotes	of
T6018	23245-23248	DT	denotes	the
T6020	23249-23255	NN	denotes	plasma
T6019	23256-23264	NN	denotes	membrane
T6021	23265-23268	CC	denotes	and
T6022	23269-23272	DT	denotes	the
T6024	23273-23281	NN	denotes	membrane
T6025	23282-23292	VBN	denotes	associated
T6023	23293-23297	NN	denotes	part
T6026	23298-23300	IN	denotes	of
T6027	23301-23304	DT	denotes	the
T6028	23305-23317	NN	denotes	cytoskeleton
T6029	23318-23319	-LRB-	denotes	[
T6030	23319-23321	CD	denotes	40
T6031	23321-23322	SYM	denotes	-
T6032	23322-23324	CD	denotes	43
T6033	23324-23325	-RRB-	denotes	]
T6034	23325-23326	.	denotes	.
T6035	23326-23538	sentence	denotes	Major constituents of the membrane skeleton are spectrin tetramers linked together by short actin filaments and several other proteins covering the entire cytoplasmic surface of the red blood cell membrane [44].
T6036	23327-23332	JJ	denotes	Major
T6037	23333-23345	NNS	denotes	constituents
T6039	23346-23348	IN	denotes	of
T6040	23349-23352	DT	denotes	the
T6042	23353-23361	NN	denotes	membrane
T6041	23362-23370	NN	denotes	skeleton
T6038	23371-23374	VBP	denotes	are
T6043	23375-23383	NN	denotes	spectrin
T6044	23384-23393	NNS	denotes	tetramers
T6045	23394-23400	VBN	denotes	linked
T6046	23401-23409	RB	denotes	together
T6047	23410-23412	IN	denotes	by
T6048	23413-23418	JJ	denotes	short
T6050	23419-23424	NN	denotes	actin
T6049	23425-23434	NNS	denotes	filaments
T6051	23435-23438	CC	denotes	and
T6052	23439-23446	JJ	denotes	several
T6054	23447-23452	JJ	denotes	other
T6053	23453-23461	NN	denotes	proteins
T6055	23462-23470	VBG	denotes	covering
T6056	23471-23474	DT	denotes	the
T6058	23475-23481	JJ	denotes	entire
T6059	23482-23493	JJ	denotes	cytoplasmic
T6057	23494-23501	NN	denotes	surface
T6060	23502-23504	IN	denotes	of
T6061	23505-23508	DT	denotes	the
T6063	23509-23512	JJ	denotes	red
T6065	23513-23518	NN	denotes	blood
T6064	23519-23523	NN	denotes	cell
T6062	23524-23532	NN	denotes	membrane
T6066	23533-23534	-LRB-	denotes	[
T6067	23534-23536	CD	denotes	44
T6068	23536-23537	-RRB-	denotes	]
T6069	23537-23538	.	denotes	.
T6070	23538-23800	sentence	denotes	Membrane cholesterol diminishes red blood cell haemolysis by the complement complex, whereas depletion of raft cholesterol abrogates association of all raft proteins with no significant effect on areas in the rest of the cell membrane and deformability [45,46].
T6071	23539-23547	NN	denotes	Membrane
T6072	23548-23559	NN	denotes	cholesterol
T6073	23560-23570	VBZ	denotes	diminishes
T6074	23571-23574	JJ	denotes	red
T6076	23575-23580	NN	denotes	blood
T6075	23581-23585	NN	denotes	cell
T6077	23586-23596	NN	denotes	haemolysis
T6078	23597-23599	IN	denotes	by
T6079	23600-23603	DT	denotes	the
T6081	23604-23614	NN	denotes	complement
T6080	23615-23622	NN	denotes	complex
T6082	23622-23624	,	denotes	,
T6083	23624-23631	IN	denotes	whereas
T6085	23632-23641	NN	denotes	depletion
T6086	23642-23644	IN	denotes	of
T6087	23645-23649	NN	denotes	raft
T6088	23650-23661	NN	denotes	cholesterol
T6084	23662-23671	VBZ	denotes	abrogates
T6089	23672-23683	NN	denotes	association
T6090	23684-23686	IN	denotes	of
T6091	23687-23690	DT	denotes	all
T6093	23691-23695	NN	denotes	raft
T6092	23696-23704	NN	denotes	proteins
T6094	23705-23709	IN	denotes	with
T6095	23710-23712	DT	denotes	no
T6097	23713-23724	JJ	denotes	significant
T6096	23725-23731	NN	denotes	effect
T6098	23732-23734	IN	denotes	on
T6099	23735-23740	NNS	denotes	areas
T6100	23741-23743	IN	denotes	in
T6101	23744-23747	DT	denotes	the
T6102	23748-23752	NN	denotes	rest
T6103	23753-23755	IN	denotes	of
T6104	23756-23759	DT	denotes	the
T6106	23760-23764	NN	denotes	cell
T6105	23765-23773	NN	denotes	membrane
T6107	23774-23777	CC	denotes	and
T6108	23778-23791	NN	denotes	deformability
T6109	23792-23793	-LRB-	denotes	[
T6111	23793-23795	CD	denotes	45
T6112	23795-23796	,	denotes	,
T6110	23796-23798	CD	denotes	46
T6113	23798-23799	-RRB-	denotes	]
T6114	23799-23800	.	denotes	.
T6115	23800-23904	sentence	denotes	Annexin A7 knock out mouse red blood cells show a more flat shape and have a higher osmotic resistance.
T6116	23801-23808	NN	denotes	Annexin
T6117	23809-23811	NN	denotes	A7
T6119	23812-23817	NN	denotes	knock
T6120	23818-23821	NN	denotes	out
T6121	23822-23827	NN	denotes	mouse
T6122	23828-23831	JJ	denotes	red
T6123	23832-23837	NN	denotes	blood
T6118	23838-23843	NNS	denotes	cells
T6124	23844-23848	VBP	denotes	show
T6125	23849-23850	DT	denotes	a
T6127	23851-23855	RBR	denotes	more
T6128	23856-23860	JJ	denotes	flat
T6126	23861-23866	NN	denotes	shape
T6129	23867-23870	CC	denotes	and
T6130	23871-23875	VBP	denotes	have
T6131	23876-23877	DT	denotes	a
T6133	23878-23884	JJR	denotes	higher
T6134	23885-23892	JJ	denotes	osmotic
T6132	23893-23903	NN	denotes	resistance
T6135	23903-23904	.	denotes	.
T6136	23904-24072	sentence	denotes	The presence of annexin A7 may alter the membrane flexibility in that it supports the typical biconcave membrane shape and leads to a lower minimal osmotic resistance.
T6137	23905-23908	DT	denotes	The
T6138	23909-23917	NN	denotes	presence
T6140	23918-23920	IN	denotes	of
T6141	23921-23928	NN	denotes	annexin
T6142	23929-23931	NN	denotes	A7
T6143	23932-23935	MD	denotes	may
T6139	23936-23941	VB	denotes	alter
T6144	23942-23945	DT	denotes	the
T6146	23946-23954	NN	denotes	membrane
T6145	23955-23966	NN	denotes	flexibility
T6147	23967-23969	IN	denotes	in
T6149	23970-23974	IN	denotes	that
T6150	23975-23977	PRP	denotes	it
T6148	23978-23986	VBZ	denotes	supports
T6151	23987-23990	DT	denotes	the
T6153	23991-23998	JJ	denotes	typical
T6154	23999-24008	NN	denotes	biconcave
T6155	24009-24017	NN	denotes	membrane
T6152	24018-24023	NN	denotes	shape
T6156	24024-24027	CC	denotes	and
T6157	24028-24033	VBZ	denotes	leads
T6158	24034-24036	IN	denotes	to
T6159	24037-24038	DT	denotes	a
T6161	24039-24044	JJR	denotes	lower
T6162	24045-24052	JJ	denotes	minimal
T6163	24053-24060	JJ	denotes	osmotic
T6160	24061-24071	NN	denotes	resistance
T6164	24071-24072	.	denotes	.
T6165	24072-24212	sentence	denotes	Annexin A7 does not appear to have an influence on plasma membrane integrity itself as the maximal osmotic resistance value stays constant.
T6166	24073-24080	NN	denotes	Annexin
T6167	24081-24083	NN	denotes	A7
T6169	24084-24088	VBZ	denotes	does
T6170	24089-24092	RB	denotes	not
T6168	24093-24099	VB	denotes	appear
T6171	24100-24102	TO	denotes	to
T6172	24103-24107	VB	denotes	have
T6173	24108-24110	DT	denotes	an
T6174	24111-24120	NN	denotes	influence
T6175	24121-24123	IN	denotes	on
T6176	24124-24130	NN	denotes	plasma
T6177	24131-24139	NN	denotes	membrane
T6178	24140-24149	NN	denotes	integrity
T6179	24150-24156	PRP	denotes	itself
T6180	24157-24159	IN	denotes	as
T6182	24160-24163	DT	denotes	the
T6184	24164-24171	JJ	denotes	maximal
T6185	24172-24179	JJ	denotes	osmotic
T6186	24180-24190	NN	denotes	resistance
T6183	24191-24196	NN	denotes	value
T6181	24197-24202	VBZ	denotes	stays
T6187	24203-24211	JJ	denotes	constant
T6188	24211-24212	.	denotes	.
T6189	24212-24333	sentence	denotes	Ca2+ and membrane skeletal proteins are known to play key roles on the red blood cell membrane shape and stability [47].
T6190	24213-24217	NN	denotes	Ca2+
T6192	24218-24221	CC	denotes	and
T6193	24222-24230	NN	denotes	membrane
T6194	24231-24239	JJ	denotes	skeletal
T6191	24240-24248	NN	denotes	proteins
T6196	24249-24252	VBP	denotes	are
T6195	24253-24258	VBN	denotes	known
T6197	24259-24261	TO	denotes	to
T6198	24262-24266	VB	denotes	play
T6199	24267-24270	JJ	denotes	key
T6200	24271-24276	NNS	denotes	roles
T6201	24277-24279	IN	denotes	on
T6202	24280-24283	DT	denotes	the
T6204	24284-24287	JJ	denotes	red
T6206	24288-24293	NN	denotes	blood
T6205	24294-24298	NN	denotes	cell
T6207	24299-24307	NN	denotes	membrane
T6203	24308-24313	NN	denotes	shape
T6208	24314-24317	CC	denotes	and
T6209	24318-24327	NN	denotes	stability
T6210	24328-24329	-LRB-	denotes	[
T6211	24329-24331	CD	denotes	47
T6212	24331-24332	-RRB-	denotes	]
T6213	24332-24333	.	denotes	.
T6214	24333-24448	sentence	denotes	Annexins have been reported to bind directly to F-actin, we could however exclude such an activity for annexin A7.
T6215	24334-24342	NNS	denotes	Annexins
T6217	24343-24347	VBP	denotes	have
T6218	24348-24352	VBN	denotes	been
T6216	24353-24361	VBN	denotes	reported
T6220	24362-24364	TO	denotes	to
T6221	24365-24369	VB	denotes	bind
T6222	24370-24378	RB	denotes	directly
T6223	24379-24381	IN	denotes	to
T6224	24382-24383	NN	denotes	F
T6226	24383-24384	HYPH	denotes	-
T6225	24384-24389	NN	denotes	actin
T6227	24389-24391	,	denotes	,
T6228	24391-24393	PRP	denotes	we
T6229	24394-24399	MD	denotes	could
T6230	24400-24407	RB	denotes	however
T6219	24408-24415	VB	denotes	exclude
T6231	24416-24420	JJ	denotes	such
T6233	24421-24423	DT	denotes	an
T6232	24424-24432	NN	denotes	activity
T6234	24433-24436	IN	denotes	for
T6235	24437-24444	NN	denotes	annexin
T6236	24445-24447	NN	denotes	A7
T6237	24447-24448	.	denotes	.
T6238	24448-24709	sentence	denotes	Nevertheless it might interact with other components and have a role in organizing the membrane cytoskeleton, controlling raft protein associations or influencing the ionic strength of red blood cells on its own or by interfering with other signaling pathways.
T6239	24449-24461	RB	denotes	Nevertheless
T6241	24462-24464	PRP	denotes	it
T6242	24465-24470	MD	denotes	might
T6240	24471-24479	VB	denotes	interact
T6243	24480-24484	IN	denotes	with
T6244	24485-24490	JJ	denotes	other
T6245	24491-24501	NNS	denotes	components
T6246	24502-24505	CC	denotes	and
T6247	24506-24510	VBP	denotes	have
T6248	24511-24512	DT	denotes	a
T6249	24513-24517	NN	denotes	role
T6250	24518-24520	IN	denotes	in
T6251	24521-24531	VBG	denotes	organizing
T6252	24532-24535	DT	denotes	the
T6254	24536-24544	NN	denotes	membrane
T6253	24545-24557	NN	denotes	cytoskeleton
T6255	24557-24559	,	denotes	,
T6256	24559-24570	VBG	denotes	controlling
T6257	24571-24575	NN	denotes	raft
T6258	24576-24583	NN	denotes	protein
T6259	24584-24596	NNS	denotes	associations
T6260	24597-24599	CC	denotes	or
T6261	24600-24611	VBG	denotes	influencing
T6262	24612-24615	DT	denotes	the
T6264	24616-24621	JJ	denotes	ionic
T6263	24622-24630	NN	denotes	strength
T6265	24631-24633	IN	denotes	of
T6266	24634-24637	JJ	denotes	red
T6268	24638-24643	NN	denotes	blood
T6267	24644-24649	NNS	denotes	cells
T6269	24650-24652	IN	denotes	on
T6270	24653-24656	PRP$	denotes	its
T6271	24657-24660	JJ	denotes	own
T6272	24661-24663	CC	denotes	or
T6273	24664-24666	IN	denotes	by
T6274	24667-24678	VBG	denotes	interfering
T6275	24679-24683	IN	denotes	with
T6276	24684-24689	JJ	denotes	other
T6278	24690-24699	NN	denotes	signaling
T6277	24700-24708	NNS	denotes	pathways
T6279	24708-24709	.	denotes	.
T6280	24709-24794	sentence	denotes	As discussed above annexin A7 could be involved in the cytoplasmic Ca2+ homeostasis.
T6281	24710-24712	IN	denotes	As
T6282	24713-24722	VBN	denotes	discussed
T6284	24723-24728	RB	denotes	above
T6285	24729-24736	NN	denotes	annexin
T6286	24737-24739	NN	denotes	A7
T6287	24740-24745	MD	denotes	could
T6288	24746-24748	VB	denotes	be
T6283	24749-24757	VBN	denotes	involved
T6289	24758-24760	IN	denotes	in
T6290	24761-24764	DT	denotes	the
T6292	24765-24776	JJ	denotes	cytoplasmic
T6293	24777-24781	NN	denotes	Ca2+
T6291	24782-24793	NN	denotes	homeostasis
T6294	24793-24794	.	denotes	.
T6295	24794-24986	sentence	denotes	It has been demonstrated that micromolar changes of the intracellular Ca2+ concentration exert a profound effect on the membrane properties that regulate red blood cell deformability [48,49].
T6296	24795-24797	PRP	denotes	It
T6298	24798-24801	VBZ	denotes	has
T6299	24802-24806	VBN	denotes	been
T6297	24807-24819	VBN	denotes	demonstrated
T6300	24820-24824	IN	denotes	that
T6302	24825-24835	JJ	denotes	micromolar
T6303	24836-24843	NNS	denotes	changes
T6304	24844-24846	IN	denotes	of
T6305	24847-24850	DT	denotes	the
T6307	24851-24864	JJ	denotes	intracellular
T6308	24865-24869	NN	denotes	Ca2+
T6306	24870-24883	NN	denotes	concentration
T6301	24884-24889	VBP	denotes	exert
T6309	24890-24891	DT	denotes	a
T6311	24892-24900	JJ	denotes	profound
T6310	24901-24907	NN	denotes	effect
T6312	24908-24910	IN	denotes	on
T6313	24911-24914	DT	denotes	the
T6315	24915-24923	NN	denotes	membrane
T6314	24924-24934	NNS	denotes	properties
T6316	24935-24939	WDT	denotes	that
T6317	24940-24948	VBP	denotes	regulate
T6318	24949-24952	JJ	denotes	red
T6320	24953-24958	NN	denotes	blood
T6319	24959-24963	NN	denotes	cell
T6321	24964-24977	NN	denotes	deformability
T6322	24978-24979	-LRB-	denotes	[
T6324	24979-24981	CD	denotes	48
T6325	24981-24982	,	denotes	,
T6323	24982-24984	CD	denotes	49
T6326	24984-24985	-RRB-	denotes	]
T6327	24985-24986	.	denotes	.
T6328	24986-25129	sentence	denotes	Furthermore, the intracellular Ca2+ was shown to regulate the membrane stability through modulation of cytoskeletal protein interactions [50].
T6329	24987-24998	RB	denotes	Furthermore
T6331	24998-25000	,	denotes	,
T6332	25000-25003	DT	denotes	the
T6334	25004-25017	JJ	denotes	intracellular
T6333	25018-25022	NN	denotes	Ca2+
T6335	25023-25026	VBD	denotes	was
T6330	25027-25032	VBN	denotes	shown
T6336	25033-25035	TO	denotes	to
T6337	25036-25044	VB	denotes	regulate
T6338	25045-25048	DT	denotes	the
T6340	25049-25057	NN	denotes	membrane
T6339	25058-25067	NN	denotes	stability
T6341	25068-25075	IN	denotes	through
T6342	25076-25086	NN	denotes	modulation
T6343	25087-25089	IN	denotes	of
T6344	25090-25102	JJ	denotes	cytoskeletal
T6345	25103-25110	NN	denotes	protein
T6346	25111-25123	NNS	denotes	interactions
T6347	25124-25125	-LRB-	denotes	[
T6348	25125-25127	CD	denotes	50
T6349	25127-25128	-RRB-	denotes	]
T6350	25128-25129	.	denotes	.
T6351	25129-25239	sentence	denotes	Ca2+ also induces a transbilayer phospholipid redistribution inducing a shape change of red blood cells [51].
T6352	25130-25134	NN	denotes	Ca2+
T6354	25135-25139	RB	denotes	also
T6353	25140-25147	VBZ	denotes	induces
T6355	25148-25149	DT	denotes	a
T6357	25150-25162	NN	denotes	transbilayer
T6358	25163-25175	NN	denotes	phospholipid
T6356	25176-25190	NN	denotes	redistribution
T6359	25191-25199	VBG	denotes	inducing
T6360	25200-25201	DT	denotes	a
T6362	25202-25207	NN	denotes	shape
T6361	25208-25214	NN	denotes	change
T6363	25215-25217	IN	denotes	of
T6364	25218-25221	JJ	denotes	red
T6366	25222-25227	NN	denotes	blood
T6365	25228-25233	NNS	denotes	cells
T6367	25234-25235	-LRB-	denotes	[
T6368	25235-25237	CD	denotes	51
T6369	25237-25238	-RRB-	denotes	]
T6370	25238-25239	.	denotes	.
T6371	25239-25349	sentence	denotes	In addition, Ca2+ ions can drive spiculation of the membrane bilayer without involving the cytoskeleton [52].
T6372	25240-25242	IN	denotes	In
T6374	25243-25251	NN	denotes	addition
T6375	25251-25253	,	denotes	,
T6376	25253-25257	NN	denotes	Ca2+
T6377	25258-25262	NNS	denotes	ions
T6378	25263-25266	MD	denotes	can
T6373	25267-25272	VB	denotes	drive
T6379	25273-25284	NN	denotes	spiculation
T6380	25285-25287	IN	denotes	of
T6381	25288-25291	DT	denotes	the
T6383	25292-25300	NN	denotes	membrane
T6382	25301-25308	NN	denotes	bilayer
T6384	25309-25316	IN	denotes	without
T6385	25317-25326	VBG	denotes	involving
T6386	25327-25330	DT	denotes	the
T6387	25331-25343	NN	denotes	cytoskeleton
T6388	25344-25345	-LRB-	denotes	[
T6389	25345-25347	CD	denotes	52
T6390	25347-25348	-RRB-	denotes	]
T6391	25348-25349	.	denotes	.
T6392	25349-25427	sentence	denotes	We have also observed a defect in platelet function in the annexin A7 mutant.
T6393	25350-25352	PRP	denotes	We
T6395	25353-25357	VBP	denotes	have
T6396	25358-25362	RB	denotes	also
T6394	25363-25371	VBN	denotes	observed
T6397	25372-25373	DT	denotes	a
T6398	25374-25380	NN	denotes	defect
T6399	25381-25383	IN	denotes	in
T6400	25384-25392	NN	denotes	platelet
T6401	25393-25401	NN	denotes	function
T6402	25402-25404	IN	denotes	in
T6403	25405-25408	DT	denotes	the
T6405	25409-25416	NN	denotes	annexin
T6406	25417-25419	NN	denotes	A7
T6404	25420-25426	NN	denotes	mutant
T6407	25426-25427	.	denotes	.
T6408	25427-25571	sentence	denotes	In the platelet aggregation experiments the initial aggregation velocity was slightly, but significantly lower in platelets lacking annexin A7.
T6409	25428-25430	IN	denotes	In
T6411	25431-25434	DT	denotes	the
T6413	25435-25443	NN	denotes	platelet
T6414	25444-25455	NN	denotes	aggregation
T6412	25456-25467	NNS	denotes	experiments
T6415	25468-25471	DT	denotes	the
T6417	25472-25479	JJ	denotes	initial
T6418	25480-25491	NN	denotes	aggregation
T6416	25492-25500	NN	denotes	velocity
T6410	25501-25504	VBD	denotes	was
T6419	25505-25513	RB	denotes	slightly
T6421	25513-25515	,	denotes	,
T6422	25515-25518	CC	denotes	but
T6423	25519-25532	RB	denotes	significantly
T6420	25533-25538	JJR	denotes	lower
T6424	25539-25541	IN	denotes	in
T6425	25542-25551	NNS	denotes	platelets
T6426	25552-25559	VBG	denotes	lacking
T6427	25560-25567	NN	denotes	annexin
T6428	25568-25570	NN	denotes	A7
T6429	25570-25571	.	denotes	.
T6430	25571-25642	sentence	denotes	These data are not reflected by a change in the in vivo bleeding time.
T6431	25572-25577	DT	denotes	These
T6432	25578-25582	NNS	denotes	data
T6434	25583-25586	VBP	denotes	are
T6435	25587-25590	RB	denotes	not
T6433	25591-25600	VBN	denotes	reflected
T6436	25601-25603	IN	denotes	by
T6437	25604-25605	DT	denotes	a
T6438	25606-25612	NN	denotes	change
T6439	25613-25615	IN	denotes	in
T6440	25616-25619	DT	denotes	the
T6442	25620-25622	FW	denotes	in
T6443	25623-25627	FW	denotes	vivo
T6444	25628-25636	NN	denotes	bleeding
T6441	25637-25641	NN	denotes	time
T6445	25641-25642	.	denotes	.
T6446	25642-25813	sentence	denotes	However the reaction speed of murine platelets is much higher than those of human and due to the technical setup we might have missed larger changes in the mouse samples.
T6447	25643-25650	RB	denotes	However
T6449	25651-25654	DT	denotes	the
T6451	25655-25663	NN	denotes	reaction
T6450	25664-25669	NN	denotes	speed
T6452	25670-25672	IN	denotes	of
T6453	25673-25679	JJ	denotes	murine
T6454	25680-25689	NNS	denotes	platelets
T6448	25690-25692	VBZ	denotes	is
T6455	25693-25697	RB	denotes	much
T6456	25698-25704	JJR	denotes	higher
T6457	25705-25709	IN	denotes	than
T6458	25710-25715	DT	denotes	those
T6459	25716-25718	IN	denotes	of
T6460	25719-25724	JJ	denotes	human
T6461	25725-25728	CC	denotes	and
T6462	25729-25732	IN	denotes	due
T6464	25733-25735	IN	denotes	to
T6465	25736-25739	DT	denotes	the
T6467	25740-25749	JJ	denotes	technical
T6466	25750-25755	NN	denotes	setup
T6468	25756-25758	PRP	denotes	we
T6469	25759-25764	MD	denotes	might
T6470	25765-25769	VB	denotes	have
T6463	25770-25776	VBN	denotes	missed
T6471	25777-25783	JJR	denotes	larger
T6472	25784-25791	NNS	denotes	changes
T6473	25792-25794	IN	denotes	in
T6474	25795-25798	DT	denotes	the
T6476	25799-25804	NN	denotes	mouse
T6475	25805-25812	NNS	denotes	samples
T6477	25812-25813	.	denotes	.
T6478	25813-25971	sentence	denotes	The immediate reaction of mouse platelets and the absence of a lag phase after triggering may be caused by an immediately initiated thrombin generation [22].
T6479	25814-25817	DT	denotes	The
T6481	25818-25827	JJ	denotes	immediate
T6480	25828-25836	NN	denotes	reaction
T6483	25837-25839	IN	denotes	of
T6484	25840-25845	NN	denotes	mouse
T6485	25846-25855	NNS	denotes	platelets
T6486	25856-25859	CC	denotes	and
T6487	25860-25863	DT	denotes	the
T6488	25864-25871	NN	denotes	absence
T6489	25872-25874	IN	denotes	of
T6490	25875-25876	DT	denotes	a
T6492	25877-25880	NN	denotes	lag
T6491	25881-25886	NN	denotes	phase
T6493	25887-25892	IN	denotes	after
T6494	25893-25903	NN	denotes	triggering
T6495	25904-25907	MD	denotes	may
T6496	25908-25910	VB	denotes	be
T6482	25911-25917	VBN	denotes	caused
T6497	25918-25920	IN	denotes	by
T6498	25921-25923	DT	denotes	an
T6500	25924-25935	RB	denotes	immediately
T6501	25936-25945	VBN	denotes	initiated
T6502	25946-25954	NN	denotes	thrombin
T6499	25955-25965	NN	denotes	generation
T6503	25966-25967	-LRB-	denotes	[
T6504	25967-25969	CD	denotes	22
T6505	25969-25970	-RRB-	denotes	]
T6506	25970-25971	.	denotes	.
T6507	25971-26158	sentence	denotes	As known from the frequent human vWF-syndrome or other haemostatic diseases, small defects often are clinically silent and do not result in physiological changes under normal conditions.
T6508	25972-25974	IN	denotes	As
T6509	25975-25980	VBN	denotes	known
T6511	25981-25985	IN	denotes	from
T6512	25986-25989	DT	denotes	the
T6514	25990-25998	JJ	denotes	frequent
T6515	25999-26004	JJ	denotes	human
T6516	26005-26008	NN	denotes	vWF
T6517	26008-26009	HYPH	denotes	-
T6513	26009-26017	NN	denotes	syndrome
T6518	26018-26020	CC	denotes	or
T6519	26021-26026	JJ	denotes	other
T6521	26027-26038	JJ	denotes	haemostatic
T6520	26039-26047	NNS	denotes	diseases
T6522	26047-26049	,	denotes	,
T6523	26049-26054	JJ	denotes	small
T6524	26055-26062	NNS	denotes	defects
T6525	26063-26068	RB	denotes	often
T6510	26069-26072	VBP	denotes	are
T6526	26073-26083	RB	denotes	clinically
T6527	26084-26090	JJ	denotes	silent
T6528	26091-26094	CC	denotes	and
T6529	26095-26097	VBP	denotes	do
T6531	26098-26101	RB	denotes	not
T6530	26102-26108	VB	denotes	result
T6532	26109-26111	IN	denotes	in
T6533	26112-26125	JJ	denotes	physiological
T6534	26126-26133	NNS	denotes	changes
T6535	26134-26139	IN	denotes	under
T6536	26140-26146	JJ	denotes	normal
T6537	26147-26157	NNS	denotes	conditions
T6538	26157-26158	.	denotes	.
T6539	26158-26353	sentence	denotes	Only in situations with severe physical injury, distinct environmental influences or additional platelet attributes like the platelet antigen polymorphism [53] differences might become apparent.
T6540	26159-26163	RB	denotes	Only
T6541	26164-26166	IN	denotes	in
T6543	26167-26177	NNS	denotes	situations
T6544	26178-26182	IN	denotes	with
T6545	26183-26189	JJ	denotes	severe
T6547	26190-26198	JJ	denotes	physical
T6546	26199-26205	NN	denotes	injury
T6548	26205-26207	,	denotes	,
T6549	26207-26215	JJ	denotes	distinct
T6551	26216-26229	JJ	denotes	environmental
T6550	26230-26240	NNS	denotes	influences
T6552	26241-26243	CC	denotes	or
T6553	26244-26254	JJ	denotes	additional
T6554	26255-26263	NN	denotes	platelet
T6555	26264-26274	VBZ	denotes	attributes
T6556	26275-26279	IN	denotes	like
T6557	26280-26283	DT	denotes	the
T6559	26284-26292	NN	denotes	platelet
T6560	26293-26300	NN	denotes	antigen
T6561	26301-26313	NN	denotes	polymorphism
T6562	26314-26315	-LRB-	denotes	[
T6563	26315-26317	CD	denotes	53
T6564	26317-26318	-RRB-	denotes	]
T6558	26319-26330	NNS	denotes	differences
T6565	26331-26336	MD	denotes	might
T6542	26337-26343	VB	denotes	become
T6566	26344-26352	JJ	denotes	apparent
T6567	26352-26353	.	denotes	.
T6643	26367-26369	IN	denotes	In
T6645	26370-26374	DT	denotes	this
T6646	26375-26380	NN	denotes	paper
T6647	26381-26383	PRP	denotes	we
T6644	26384-26390	VBP	denotes	report
T6648	26391-26394	DT	denotes	the
T6649	26395-26403	NN	denotes	presence
T6650	26404-26406	IN	denotes	of
T6651	26407-26411	CC	denotes	both
T6653	26412-26419	NN	denotes	annexin
T6654	26420-26422	NN	denotes	A7
T6652	26423-26431	NNS	denotes	isoforms
T6655	26432-26434	IN	denotes	in
T6656	26435-26438	JJ	denotes	red
T6658	26439-26444	NN	denotes	blood
T6657	26445-26450	NNS	denotes	cells
T6659	26451-26456	WRB	denotes	where
T6661	26457-26461	PRP	denotes	they
T6660	26462-26465	VBP	denotes	are
T6662	26466-26474	JJ	denotes	abundant
T6663	26475-26477	IN	denotes	in
T6664	26478-26490	NNS	denotes	nanovesicles
T6665	26491-26495	WDT	denotes	that
T6666	26496-26500	VBP	denotes	form
T6667	26501-26505	IN	denotes	upon
T6668	26506-26510	NN	denotes	Ca2+
T6669	26511-26519	NN	denotes	addition
T6670	26519-26520	.	denotes	.
T6671	26520-26588	sentence	denotes	The proteins are however not required for the vesiculation process.
T6672	26521-26524	DT	denotes	The
T6673	26525-26533	NN	denotes	proteins
T6675	26534-26537	VBP	denotes	are
T6676	26538-26545	RB	denotes	however
T6677	26546-26549	RB	denotes	not
T6674	26550-26558	VBN	denotes	required
T6678	26559-26562	IN	denotes	for
T6679	26563-26566	DT	denotes	the
T6681	26567-26579	NN	denotes	vesiculation
T6680	26580-26587	NN	denotes	process
T6682	26587-26588	.	denotes	.
T6683	26588-26852	sentence	denotes	They are also not essential for the formation of specific membrane domains such as lipid rafts, as exogenously added recombinant annexin A7 redistributed to similar positions in a density gradient containing membranes derived from red blood cells of anxA7-/-mice.
T6684	26589-26593	PRP	denotes	They
T6685	26594-26597	VBP	denotes	are
T6686	26598-26602	RB	denotes	also
T6687	26603-26606	RB	denotes	not
T6688	26607-26616	JJ	denotes	essential
T6689	26617-26620	IN	denotes	for
T6690	26621-26624	DT	denotes	the
T6691	26625-26634	NN	denotes	formation
T6692	26635-26637	IN	denotes	of
T6693	26638-26646	JJ	denotes	specific
T6695	26647-26655	NN	denotes	membrane
T6694	26656-26663	NNS	denotes	domains
T6696	26664-26668	JJ	denotes	such
T6697	26669-26671	IN	denotes	as
T6698	26672-26677	NN	denotes	lipid
T6699	26678-26683	NNS	denotes	rafts
T6700	26683-26685	,	denotes	,
T6701	26685-26687	IN	denotes	as
T6702	26688-26699	RB	denotes	exogenously
T6703	26700-26705	VBN	denotes	added
T6705	26706-26717	JJ	denotes	recombinant
T6706	26718-26725	NN	denotes	annexin
T6704	26726-26728	NN	denotes	A7
T6707	26729-26742	VBN	denotes	redistributed
T6708	26743-26745	IN	denotes	to
T6709	26746-26753	JJ	denotes	similar
T6710	26754-26763	NNS	denotes	positions
T6711	26764-26766	IN	denotes	in
T6712	26767-26768	DT	denotes	a
T6714	26769-26776	NN	denotes	density
T6713	26777-26785	NN	denotes	gradient
T6715	26786-26796	VBG	denotes	containing
T6716	26797-26806	NNS	denotes	membranes
T6717	26807-26814	VBN	denotes	derived
T6718	26815-26819	IN	denotes	from
T6719	26820-26823	JJ	denotes	red
T6721	26824-26829	NN	denotes	blood
T6720	26830-26835	NNS	denotes	cells
T6722	26836-26838	IN	denotes	of
T6723	26839-26844	NN	denotes	anxA7
T6725	26844-26845	SYM	denotes	-
T6726	26845-26846	HYPH	denotes	/
T6727	26846-26847	SYM	denotes	-
T6724	26847-26851	NNS	denotes	mice
T6728	26851-26852	.	denotes	.
T6729	26852-26979	sentence	denotes	The observed cell shape change of erythrocytes lacking annexin A7 might be due to alterations in the erythrocyte cytoskeleton.
T6730	26853-26856	DT	denotes	The
T6732	26857-26865	VBN	denotes	observed
T6733	26866-26870	NN	denotes	cell
T6734	26871-26876	NN	denotes	shape
T6731	26877-26883	NN	denotes	change
T6736	26884-26886	IN	denotes	of
T6737	26887-26899	NNS	denotes	erythrocytes
T6738	26900-26907	VBG	denotes	lacking
T6739	26908-26915	NN	denotes	annexin
T6740	26916-26918	NN	denotes	A7
T6741	26919-26924	MD	denotes	might
T6735	26925-26927	VB	denotes	be
T6742	26928-26931	IN	denotes	due
T6743	26932-26934	IN	denotes	to
T6744	26935-26946	NNS	denotes	alterations
T6745	26947-26949	IN	denotes	in
T6746	26950-26953	DT	denotes	the
T6748	26954-26965	NN	denotes	erythrocyte
T6747	26966-26978	NN	denotes	cytoskeleton
T6749	26978-26979	.	denotes	.
T6750	26979-27097	sentence	denotes	As a direct interaction with F-actin was ruled out, this effect might be mediated by annexin's Ca2+-binding activity.
T6751	26980-26982	IN	denotes	As
T6753	26983-26984	DT	denotes	a
T6755	26985-26991	JJ	denotes	direct
T6754	26992-27003	NN	denotes	interaction
T6756	27004-27008	IN	denotes	with
T6757	27009-27010	NN	denotes	F
T6759	27010-27011	HYPH	denotes	-
T6758	27011-27016	NN	denotes	actin
T6760	27017-27020	VBD	denotes	was
T6752	27021-27026	VBN	denotes	ruled
T6762	27027-27030	RP	denotes	out
T6763	27030-27032	,	denotes	,
T6764	27032-27036	DT	denotes	this
T6765	27037-27043	NN	denotes	effect
T6766	27044-27049	MD	denotes	might
T6767	27050-27052	VB	denotes	be
T6761	27053-27061	VBN	denotes	mediated
T6768	27062-27064	IN	denotes	by
T6769	27065-27072	NN	denotes	annexin
T6771	27072-27074	POS	denotes	's
T6772	27075-27079	NN	denotes	Ca2+
T6774	27079-27080	HYPH	denotes	-
T6773	27080-27087	VBG	denotes	binding
T6770	27088-27096	NN	denotes	activity
T6775	27096-27097	.	denotes	.
T6879	27108-27119	NN	denotes	Acquisition
T6880	27120-27122	IN	denotes	of
T6881	27123-27129	JJ	denotes	murine
T6883	27130-27133	CC	denotes	and
T6884	27134-27139	JJ	denotes	human
T6882	27140-27145	NN	denotes	blood
T6885	27146-27153	NNS	denotes	samples
T6886	27153-27441	sentence	denotes	Blood from 129Sv wild type and annexin A7 knock out mice (anxA7-/-) kept under pathogen-free conditions and fed with regular food, was collected in 1 ml syringes with a 22 gauge needle punctating the right cardiac ventricle immediately after they had been killed by cervical dislocation.
T6887	27154-27159	NN	denotes	Blood
T6889	27160-27164	IN	denotes	from
T6890	27165-27170	NN	denotes	129Sv
T6892	27171-27175	JJ	denotes	wild
T6893	27176-27180	NN	denotes	type
T6894	27181-27184	CC	denotes	and
T6895	27185-27192	NN	denotes	annexin
T6896	27193-27195	NN	denotes	A7
T6897	27196-27201	NN	denotes	knock
T6898	27202-27205	NN	denotes	out
T6891	27206-27210	NNS	denotes	mice
T6899	27211-27212	-LRB-	denotes	(
T6900	27212-27217	NN	denotes	anxA7
T6901	27217-27218	SYM	denotes	-
T6902	27218-27219	HYPH	denotes	/
T6903	27219-27220	SYM	denotes	-
T6904	27220-27221	-RRB-	denotes	)
T6905	27222-27226	VBN	denotes	kept
T6906	27227-27232	IN	denotes	under
T6907	27233-27241	NN	denotes	pathogen
T6909	27241-27242	HYPH	denotes	-
T6908	27242-27246	JJ	denotes	free
T6910	27247-27257	NNS	denotes	conditions
T6911	27258-27261	CC	denotes	and
T6912	27262-27265	VBN	denotes	fed
T6913	27266-27270	IN	denotes	with
T6914	27271-27278	JJ	denotes	regular
T6915	27279-27283	NN	denotes	food
T6916	27283-27285	,	denotes	,
T6917	27285-27288	VBD	denotes	was
T6888	27289-27298	VBN	denotes	collected
T6918	27299-27301	IN	denotes	in
T6919	27302-27303	CD	denotes	1
T6920	27304-27306	NN	denotes	ml
T6921	27307-27315	NNS	denotes	syringes
T6922	27316-27320	IN	denotes	with
T6923	27321-27322	DT	denotes	a
T6925	27323-27325	CD	denotes	22
T6926	27326-27331	NN	denotes	gauge
T6924	27332-27338	NN	denotes	needle
T6927	27339-27349	VBG	denotes	punctating
T6928	27350-27353	DT	denotes	the
T6930	27354-27359	JJ	denotes	right
T6931	27360-27367	JJ	denotes	cardiac
T6929	27368-27377	NN	denotes	ventricle
T6932	27378-27389	RB	denotes	immediately
T6934	27390-27395	IN	denotes	after
T6935	27396-27400	PRP	denotes	they
T6936	27401-27404	VBD	denotes	had
T6937	27405-27409	VBN	denotes	been
T6933	27410-27416	VBN	denotes	killed
T6938	27417-27419	IN	denotes	by
T6939	27420-27428	JJ	denotes	cervical
T6940	27429-27440	NN	denotes	dislocation
T6941	27440-27441	.	denotes	.
T6942	27441-27551	sentence	denotes	Coagulation generally was inhibited with 1/10 volume of 10 mM NaEDTA, pH 7.5, already present in the syringe.
T6943	27442-27453	NN	denotes	Coagulation
T6945	27454-27463	RB	denotes	generally
T6946	27464-27467	VBD	denotes	was
T6944	27468-27477	VBN	denotes	inhibited
T6947	27478-27482	IN	denotes	with
T6948	27483-27484	CD	denotes	1
T6950	27484-27485	SYM	denotes	/
T6949	27485-27487	CD	denotes	10
T6951	27488-27494	NN	denotes	volume
T6952	27495-27497	IN	denotes	of
T6953	27498-27500	CD	denotes	10
T6954	27501-27503	NN	denotes	mM
T6955	27504-27510	NN	denotes	NaEDTA
T6956	27510-27512	,	denotes	,
T6957	27512-27514	NN	denotes	pH
T6958	27515-27518	CD	denotes	7.5
T6959	27518-27520	,	denotes	,
T6960	27520-27527	RB	denotes	already
T6961	27528-27535	JJ	denotes	present
T6962	27536-27538	IN	denotes	in
T6963	27539-27542	DT	denotes	the
T6964	27543-27550	NN	denotes	syringe
T6965	27550-27551	.	denotes	.
T6966	27551-27671	sentence	denotes	The mice were killed by cervical dislocation in order to avoid any effect of anaesthetic chemicals on blood parameters.
T6967	27552-27555	DT	denotes	The
T6968	27556-27560	NNS	denotes	mice
T6970	27561-27565	VBD	denotes	were
T6969	27566-27572	VBN	denotes	killed
T6971	27573-27575	IN	denotes	by
T6972	27576-27584	JJ	denotes	cervical
T6973	27585-27596	NN	denotes	dislocation
T6974	27597-27599	IN	denotes	in
T6975	27600-27605	NN	denotes	order
T6976	27606-27608	TO	denotes	to
T6977	27609-27614	VB	denotes	avoid
T6978	27615-27618	DT	denotes	any
T6979	27619-27625	NN	denotes	effect
T6980	27626-27628	IN	denotes	of
T6981	27629-27640	JJ	denotes	anaesthetic
T6982	27641-27650	NNS	denotes	chemicals
T6983	27651-27653	IN	denotes	on
T6984	27654-27659	NN	denotes	blood
T6985	27660-27670	NNS	denotes	parameters
T6986	27670-27671	.	denotes	.
T6987	27671-27751	sentence	denotes	The wild type and mutant mice were matched regarding their age, weight and sex.
T6988	27672-27675	DT	denotes	The
T6990	27676-27680	JJ	denotes	wild
T6991	27681-27685	NN	denotes	type
T6992	27686-27689	CC	denotes	and
T6993	27690-27696	JJ	denotes	mutant
T6989	27697-27701	NNS	denotes	mice
T6995	27702-27706	VBD	denotes	were
T6994	27707-27714	VBN	denotes	matched
T6996	27715-27724	VBG	denotes	regarding
T6997	27725-27730	PRP$	denotes	their
T6998	27731-27734	NN	denotes	age
T6999	27734-27736	,	denotes	,
T7000	27736-27742	NN	denotes	weight
T7001	27743-27746	CC	denotes	and
T7002	27747-27750	NN	denotes	sex
T7003	27750-27751	.	denotes	.
T7004	27751-27863	sentence	denotes	The daytime of blood collection, sampling site and collection method were kept constant during all experiments.
T7005	27752-27755	DT	denotes	The
T7006	27756-27763	NN	denotes	daytime
T7008	27764-27766	IN	denotes	of
T7009	27767-27772	NN	denotes	blood
T7010	27773-27783	NN	denotes	collection
T7011	27783-27785	,	denotes	,
T7012	27785-27793	VBG	denotes	sampling
T7013	27794-27798	NN	denotes	site
T7014	27799-27802	CC	denotes	and
T7015	27803-27813	NN	denotes	collection
T7016	27814-27820	NN	denotes	method
T7017	27821-27825	VBD	denotes	were
T7007	27826-27830	VBN	denotes	kept
T7018	27831-27839	JJ	denotes	constant
T7019	27840-27846	IN	denotes	during
T7020	27847-27850	DT	denotes	all
T7021	27851-27862	NNS	denotes	experiments
T7022	27862-27863	.	denotes	.
T7023	27863-27968	sentence	denotes	A typical murine blood sample volume consisted of 550 μl, with variations ranging from 200 μl to 900 μl.
T7024	27864-27865	DT	denotes	A
T7026	27866-27873	JJ	denotes	typical
T7027	27874-27880	JJ	denotes	murine
T7029	27881-27886	NN	denotes	blood
T7028	27887-27893	NN	denotes	sample
T7025	27894-27900	NN	denotes	volume
T7030	27901-27910	VBD	denotes	consisted
T7031	27911-27913	IN	denotes	of
T7032	27914-27917	CD	denotes	550
T7033	27918-27920	NN	denotes	μl
T7034	27920-27922	,	denotes	,
T7035	27922-27926	IN	denotes	with
T7036	27927-27937	NNS	denotes	variations
T7037	27938-27945	VBG	denotes	ranging
T7038	27946-27950	IN	denotes	from
T7039	27951-27954	CD	denotes	200
T7040	27955-27957	NNS	denotes	μl
T7041	27958-27960	IN	denotes	to
T7042	27961-27964	CD	denotes	900
T7043	27965-27967	NNS	denotes	μl
T7044	27967-27968	.	denotes	.
T7045	27968-28078	sentence	denotes	Human blood samples from the authors were collected by vein puncture using common EDTA-monovettes (Sarstedt).
T7046	27969-27974	JJ	denotes	Human
T7048	27975-27980	NN	denotes	blood
T7047	27981-27988	NNS	denotes	samples
T7050	27989-27993	IN	denotes	from
T7051	27994-27997	DT	denotes	the
T7052	27998-28005	NNS	denotes	authors
T7053	28006-28010	VBD	denotes	were
T7049	28011-28020	VBN	denotes	collected
T7054	28021-28023	IN	denotes	by
T7055	28024-28028	NN	denotes	vein
T7056	28029-28037	NN	denotes	puncture
T7057	28038-28043	VBG	denotes	using
T7058	28044-28050	JJ	denotes	common
T7060	28051-28055	NN	denotes	EDTA
T7061	28055-28056	HYPH	denotes	-
T7059	28056-28066	NNS	denotes	monovettes
T7062	28067-28068	-LRB-	denotes	(
T7063	28068-28076	NNP	denotes	Sarstedt
T7064	28076-28077	-RRB-	denotes	)
T7065	28077-28078	.	denotes	.
T7066	28078-28271	sentence	denotes	We performed our studies using well standardized sampling conditions, as differences in the blood sampling site, for example between tail and heart, show large changes in all cell type counts.
T7067	28079-28081	PRP	denotes	We
T7068	28082-28091	VBD	denotes	performed
T7069	28092-28095	PRP$	denotes	our
T7070	28096-28103	NNS	denotes	studies
T7071	28104-28109	VBG	denotes	using
T7072	28110-28114	RB	denotes	well
T7073	28115-28127	JJ	denotes	standardized
T7075	28128-28136	NN	denotes	sampling
T7074	28137-28147	NNS	denotes	conditions
T7076	28147-28149	,	denotes	,
T7077	28149-28151	IN	denotes	as
T7079	28152-28163	NNS	denotes	differences
T7080	28164-28166	IN	denotes	in
T7081	28167-28170	DT	denotes	the
T7083	28171-28176	NN	denotes	blood
T7084	28177-28185	NN	denotes	sampling
T7082	28186-28190	NN	denotes	site
T7085	28190-28192	,	denotes	,
T7086	28192-28195	IN	denotes	for
T7088	28196-28203	NN	denotes	example
T7087	28204-28211	IN	denotes	between
T7089	28212-28216	NN	denotes	tail
T7090	28217-28220	CC	denotes	and
T7091	28221-28226	NN	denotes	heart
T7092	28226-28228	,	denotes	,
T7078	28228-28232	VBP	denotes	show
T7093	28233-28238	JJ	denotes	large
T7094	28239-28246	NNS	denotes	changes
T7095	28247-28249	IN	denotes	in
T7096	28250-28253	DT	denotes	all
T7098	28254-28258	NN	denotes	cell
T7099	28259-28263	NN	denotes	type
T7097	28264-28270	NNS	denotes	counts
T7100	28270-28271	.	denotes	.
T7101	28271-28337	sentence	denotes	First and second sample draw affect blood measurements, too [22].
T7102	28272-28277	JJ	denotes	First
T7104	28278-28281	CC	denotes	and
T7105	28282-28288	JJ	denotes	second
T7106	28289-28295	NN	denotes	sample
T7103	28296-28300	NN	denotes	draw
T7107	28301-28307	VBP	denotes	affect
T7108	28308-28313	NN	denotes	blood
T7109	28314-28326	NNS	denotes	measurements
T7110	28326-28328	,	denotes	,
T7111	28328-28331	RB	denotes	too
T7112	28332-28333	-LRB-	denotes	[
T7113	28333-28335	CD	denotes	22
T7114	28335-28336	-RRB-	denotes	]
T7115	28336-28337	.	denotes	.
T7116	28337-28429	sentence	denotes	Also the genetic background in common laboratory and transgenic mice affects the phenotype.
T7117	28338-28342	RB	denotes	Also
T7119	28343-28346	DT	denotes	the
T7121	28347-28354	JJ	denotes	genetic
T7120	28355-28365	NN	denotes	background
T7122	28366-28368	IN	denotes	in
T7123	28369-28375	JJ	denotes	common
T7125	28376-28386	NN	denotes	laboratory
T7126	28387-28390	CC	denotes	and
T7127	28391-28401	JJ	denotes	transgenic
T7124	28402-28406	NNS	denotes	mice
T7118	28407-28414	VBZ	denotes	affects
T7128	28415-28418	DT	denotes	the
T7129	28419-28428	NN	denotes	phenotype
T7130	28428-28429	.	denotes	.
T7131	28429-28536	sentence	denotes	A change from one strain to another may protect mice from effects of the primarily induced genetic defect.
T7132	28430-28431	DT	denotes	A
T7133	28432-28438	NN	denotes	change
T7135	28439-28443	IN	denotes	from
T7136	28444-28447	CD	denotes	one
T7137	28448-28454	NN	denotes	strain
T7138	28455-28457	IN	denotes	to
T7139	28458-28465	DT	denotes	another
T7140	28466-28469	MD	denotes	may
T7134	28470-28477	VB	denotes	protect
T7141	28478-28482	NNS	denotes	mice
T7142	28483-28487	IN	denotes	from
T7143	28488-28495	NNS	denotes	effects
T7144	28496-28498	IN	denotes	of
T7145	28499-28502	DT	denotes	the
T7147	28503-28512	RB	denotes	primarily
T7148	28513-28520	VBN	denotes	induced
T7149	28521-28528	JJ	denotes	genetic
T7146	28529-28535	NN	denotes	defect
T7150	28535-28536	.	denotes	.
T7151	28536-28696	sentence	denotes	For example, BALB/c mice have a significantly increased velocity of platelet aggregation as compared to 129Sv mice, which were used throughout this study [23].
T7152	28537-28540	IN	denotes	For
T7154	28541-28548	NN	denotes	example
T7155	28548-28550	,	denotes	,
T7156	28550-28554	NN	denotes	BALB
T7158	28554-28555	HYPH	denotes	/
T7157	28555-28556	NN	denotes	c
T7159	28557-28561	NNS	denotes	mice
T7153	28562-28566	VBP	denotes	have
T7160	28567-28568	DT	denotes	a
T7162	28569-28582	RB	denotes	significantly
T7163	28583-28592	VBN	denotes	increased
T7161	28593-28601	NN	denotes	velocity
T7164	28602-28604	IN	denotes	of
T7165	28605-28613	NN	denotes	platelet
T7166	28614-28625	NN	denotes	aggregation
T7167	28626-28628	IN	denotes	as
T7168	28629-28637	VBN	denotes	compared
T7169	28638-28640	IN	denotes	to
T7170	28641-28646	NN	denotes	129Sv
T7171	28647-28651	NNS	denotes	mice
T7172	28651-28653	,	denotes	,
T7173	28653-28658	WDT	denotes	which
T7175	28659-28663	VBD	denotes	were
T7174	28664-28668	VBN	denotes	used
T7176	28669-28679	IN	denotes	throughout
T7177	28680-28684	DT	denotes	this
T7178	28685-28690	NN	denotes	study
T7179	28691-28692	-LRB-	denotes	[
T7180	28692-28694	CD	denotes	23
T7181	28694-28695	-RRB-	denotes	]
T7182	28695-28696	.	denotes	.
T7273	28698-28702	NN	denotes	Self
T7274	28703-28710	VBG	denotes	forming
T7276	28711-28720	NN	denotes	iodixanol
T7277	28721-28728	NN	denotes	density
T7275	28729-28738	NNS	denotes	gradients
T7278	28739-28741	IN	denotes	of
T7279	28742-28747	JJ	denotes	human
T7281	28748-28751	JJ	denotes	red
T7282	28752-28757	NN	denotes	blood
T7280	28758-28763	NNS	denotes	cells
T7283	28763-28839	sentence	denotes	The subcellular distribution of annexin A7 was addressed according to [24].
T7284	28764-28767	DT	denotes	The
T7286	28768-28779	JJ	denotes	subcellular
T7285	28780-28792	NN	denotes	distribution
T7288	28793-28795	IN	denotes	of
T7289	28796-28803	NN	denotes	annexin
T7290	28804-28806	NN	denotes	A7
T7291	28807-28810	VBD	denotes	was
T7287	28811-28820	VBN	denotes	addressed
T7292	28821-28830	VBG	denotes	according
T7293	28831-28833	IN	denotes	to
T7294	28834-28835	-LRB-	denotes	[
T7295	28835-28837	CD	denotes	24
T7296	28837-28838	-RRB-	denotes	]
T7297	28838-28839	.	denotes	.
T7298	28839-29145	sentence	denotes	Red blood cells were centrifuged and washed in isotonic buffered salt solution, collected in ice cold lysis buffer (20 mM Hepes, pH 7.4, 200 μM CaCl2, 0.25 M sucrose, and proteinase inhibitors (Roche)), homogenized by a loosely fitting dounce homogenizer and additional passages through a 22 gauge needle.
T7299	28840-28843	JJ	denotes	Red
T7301	28844-28849	NN	denotes	blood
T7300	28850-28855	NNS	denotes	cells
T7303	28856-28860	VBD	denotes	were
T7302	28861-28872	VBN	denotes	centrifuged
T7304	28873-28876	CC	denotes	and
T7305	28877-28883	VBN	denotes	washed
T7306	28884-28886	IN	denotes	in
T7307	28887-28895	JJ	denotes	isotonic
T7309	28896-28904	VBN	denotes	buffered
T7310	28905-28909	NN	denotes	salt
T7308	28910-28918	NN	denotes	solution
T7311	28918-28920	,	denotes	,
T7312	28920-28929	VBN	denotes	collected
T7313	28930-28932	IN	denotes	in
T7314	28933-28936	NN	denotes	ice
T7315	28937-28941	JJ	denotes	cold
T7317	28942-28947	NN	denotes	lysis
T7316	28948-28954	NN	denotes	buffer
T7318	28955-28956	-LRB-	denotes	(
T7320	28956-28958	CD	denotes	20
T7321	28959-28961	NN	denotes	mM
T7319	28962-28967	NN	denotes	Hepes
T7322	28967-28969	,	denotes	,
T7323	28969-28971	NN	denotes	pH
T7324	28972-28975	CD	denotes	7.4
T7325	28975-28977	,	denotes	,
T7326	28977-28980	CD	denotes	200
T7327	28981-28983	NN	denotes	μM
T7328	28984-28989	NN	denotes	CaCl2
T7329	28989-28991	,	denotes	,
T7330	28991-28995	CD	denotes	0.25
T7331	28996-28997	NN	denotes	M
T7332	28998-29005	NN	denotes	sucrose
T7333	29005-29007	,	denotes	,
T7334	29007-29010	CC	denotes	and
T7335	29011-29021	NN	denotes	proteinase
T7336	29022-29032	NNS	denotes	inhibitors
T7337	29033-29034	-LRB-	denotes	(
T7338	29034-29039	NNP	denotes	Roche
T7339	29039-29040	-RRB-	denotes	)
T7340	29040-29041	-RRB-	denotes	)
T7341	29041-29043	,	denotes	,
T7342	29043-29054	VBN	denotes	homogenized
T7343	29055-29057	IN	denotes	by
T7344	29058-29059	DT	denotes	a
T7346	29060-29067	RB	denotes	loosely
T7347	29068-29075	JJ	denotes	fitting
T7348	29076-29082	NN	denotes	dounce
T7345	29083-29094	NN	denotes	homogenizer
T7349	29095-29098	CC	denotes	and
T7350	29099-29109	JJ	denotes	additional
T7351	29110-29118	NNS	denotes	passages
T7352	29119-29126	IN	denotes	through
T7353	29127-29128	DT	denotes	a
T7355	29129-29131	CD	denotes	22
T7356	29132-29137	NN	denotes	gauge
T7354	29138-29144	NN	denotes	needle
T7357	29144-29145	.	denotes	.
T7358	29145-29305	sentence	denotes	A 50 % iodixanol solution was established by mixing Optiprep solution (60 % iodixanol; Sigma) with buffer (120 mM Hepes, pH 7.4, 1.2 mM CaCl2, 0.25 M sucrose).
T7359	29146-29147	DT	denotes	A
T7361	29148-29150	CD	denotes	50
T7362	29151-29152	NN	denotes	%
T7363	29153-29162	NN	denotes	iodixanol
T7360	29163-29171	NN	denotes	solution
T7365	29172-29175	VBD	denotes	was
T7364	29176-29187	VBN	denotes	established
T7366	29188-29190	IN	denotes	by
T7367	29191-29197	VBG	denotes	mixing
T7368	29198-29206	NN	denotes	Optiprep
T7369	29207-29215	NN	denotes	solution
T7370	29216-29217	-LRB-	denotes	(
T7372	29217-29219	CD	denotes	60
T7373	29220-29221	NN	denotes	%
T7374	29222-29231	NN	denotes	iodixanol
T7375	29231-29232	:	denotes	;
T7371	29233-29238	NN	denotes	Sigma
T7376	29238-29239	-RRB-	denotes	)
T7377	29240-29244	IN	denotes	with
T7378	29245-29251	NN	denotes	buffer
T7379	29252-29253	-LRB-	denotes	(
T7381	29253-29256	CD	denotes	120
T7382	29257-29259	NN	denotes	mM
T7383	29260-29265	NN	denotes	Hepes
T7384	29265-29267	,	denotes	,
T7385	29267-29269	NN	denotes	pH
T7386	29270-29273	CD	denotes	7.4
T7387	29273-29275	,	denotes	,
T7388	29275-29278	CD	denotes	1.2
T7389	29279-29281	NN	denotes	mM
T7390	29282-29287	NN	denotes	CaCl2
T7391	29287-29289	,	denotes	,
T7392	29289-29293	CD	denotes	0.25
T7393	29294-29295	NN	denotes	M
T7380	29296-29303	NN	denotes	sucrose
T7394	29303-29304	-RRB-	denotes	)
T7395	29304-29305	.	denotes	.
T7396	29305-29472	sentence	denotes	Seven parts of this 50 % iodixanol solution were mixed with thirteen parts of the red blood cell solution, generating a gradient solution containing 17.5 % iodixanol.
T7397	29306-29311	CD	denotes	Seven
T7398	29312-29317	NNS	denotes	parts
T7400	29318-29320	IN	denotes	of
T7401	29321-29325	DT	denotes	this
T7403	29326-29328	CD	denotes	50
T7404	29329-29330	NN	denotes	%
T7405	29331-29340	NN	denotes	iodixanol
T7402	29341-29349	NN	denotes	solution
T7406	29350-29354	VBD	denotes	were
T7399	29355-29360	VBN	denotes	mixed
T7407	29361-29365	IN	denotes	with
T7408	29366-29374	CD	denotes	thirteen
T7409	29375-29380	NNS	denotes	parts
T7410	29381-29383	IN	denotes	of
T7411	29384-29387	DT	denotes	the
T7413	29388-29391	JJ	denotes	red
T7415	29392-29397	NN	denotes	blood
T7414	29398-29402	NN	denotes	cell
T7412	29403-29411	NN	denotes	solution
T7416	29411-29413	,	denotes	,
T7417	29413-29423	VBG	denotes	generating
T7418	29424-29425	DT	denotes	a
T7420	29426-29434	NN	denotes	gradient
T7419	29435-29443	NN	denotes	solution
T7421	29444-29454	VBG	denotes	containing
T7422	29455-29459	CD	denotes	17.5
T7423	29460-29461	NN	denotes	%
T7424	29462-29471	NN	denotes	iodixanol
T7425	29471-29472	.	denotes	.
T7426	29472-29739	sentence	denotes	The mixture was filled into a centrifuge tube underlayed by cushions of 30 % and 35 % iodixanol and centrifuged for 3 hours at 270,000 × g in a SW41 Ti swing out rotor (Beckman) to achieve a nearly linear density gradient (approximately 1.06 g/ml to 1.20 g/ml) [24].
T7427	29473-29476	DT	denotes	The
T7428	29477-29484	NN	denotes	mixture
T7430	29485-29488	VBD	denotes	was
T7429	29489-29495	VBN	denotes	filled
T7431	29496-29500	IN	denotes	into
T7432	29501-29502	DT	denotes	a
T7434	29503-29513	JJ	denotes	centrifuge
T7433	29514-29518	NN	denotes	tube
T7435	29519-29529	VBN	denotes	underlayed
T7436	29530-29532	IN	denotes	by
T7437	29533-29541	NNS	denotes	cushions
T7438	29542-29544	IN	denotes	of
T7439	29545-29547	CD	denotes	30
T7440	29548-29549	NN	denotes	%
T7442	29550-29553	CC	denotes	and
T7443	29554-29556	CD	denotes	35
T7444	29557-29558	NN	denotes	%
T7441	29559-29568	NN	denotes	iodixanol
T7445	29569-29572	CC	denotes	and
T7446	29573-29584	VBN	denotes	centrifuged
T7447	29585-29588	IN	denotes	for
T7448	29589-29590	CD	denotes	3
T7449	29591-29596	NNS	denotes	hours
T7450	29597-29599	IN	denotes	at
T7451	29600-29607	CD	denotes	270,000
T7453	29608-29609	SYM	denotes	×
T7452	29610-29611	NN	denotes	g
T7454	29612-29614	IN	denotes	in
T7455	29615-29616	DT	denotes	a
T7457	29617-29621	NN	denotes	SW41
T7458	29622-29624	NNP	denotes	Ti
T7459	29625-29630	NN	denotes	swing
T7460	29631-29634	NN	denotes	out
T7456	29635-29640	NN	denotes	rotor
T7461	29641-29642	-LRB-	denotes	(
T7462	29642-29649	NN	denotes	Beckman
T7463	29649-29650	-RRB-	denotes	)
T7464	29651-29653	TO	denotes	to
T7465	29654-29661	VB	denotes	achieve
T7466	29662-29663	DT	denotes	a
T7468	29664-29670	RB	denotes	nearly
T7469	29671-29677	JJ	denotes	linear
T7470	29678-29685	NN	denotes	density
T7467	29686-29694	NN	denotes	gradient
T7471	29695-29696	-LRB-	denotes	(
T7473	29696-29709	RB	denotes	approximately
T7474	29710-29714	CD	denotes	1.06
T7472	29715-29716	NNS	denotes	g
T7475	29716-29717	SYM	denotes	/
T7476	29717-29719	NN	denotes	ml
T7477	29720-29722	IN	denotes	to
T7478	29723-29727	CD	denotes	1.20
T7479	29728-29729	NNS	denotes	g
T7480	29729-29730	SYM	denotes	/
T7481	29730-29732	NN	denotes	ml
T7482	29732-29733	-RRB-	denotes	)
T7483	29734-29735	-LRB-	denotes	[
T7484	29735-29737	CD	denotes	24
T7485	29737-29738	-RRB-	denotes	]
T7486	29738-29739	.	denotes	.
T7487	29739-29903	sentence	denotes	Fractions of 0.6 ml were collected from the top of the gradient by a 1 ml syringe without using a needle and immediately frozen in liquid nitrogen for further use.
T7488	29740-29749	NNS	denotes	Fractions
T7490	29750-29752	IN	denotes	of
T7491	29753-29756	CD	denotes	0.6
T7492	29757-29759	NN	denotes	ml
T7493	29760-29764	VBD	denotes	were
T7489	29765-29774	VBN	denotes	collected
T7494	29775-29779	IN	denotes	from
T7495	29780-29783	DT	denotes	the
T7496	29784-29787	NN	denotes	top
T7497	29788-29790	IN	denotes	of
T7498	29791-29794	DT	denotes	the
T7499	29795-29803	NN	denotes	gradient
T7500	29804-29806	IN	denotes	by
T7501	29807-29808	DT	denotes	a
T7503	29809-29810	CD	denotes	1
T7504	29811-29813	NN	denotes	ml
T7502	29814-29821	NN	denotes	syringe
T7505	29822-29829	IN	denotes	without
T7506	29830-29835	VBG	denotes	using
T7507	29836-29837	DT	denotes	a
T7508	29838-29844	NN	denotes	needle
T7509	29845-29848	CC	denotes	and
T7510	29849-29860	RB	denotes	immediately
T7511	29861-29867	VBN	denotes	frozen
T7512	29868-29870	IN	denotes	in
T7513	29871-29877	JJ	denotes	liquid
T7514	29878-29886	NN	denotes	nitrogen
T7515	29887-29890	IN	denotes	for
T7516	29891-29898	JJ	denotes	further
T7517	29899-29902	NN	denotes	use
T7518	29902-29903	.	denotes	.
T7632	29905-29915	NN	denotes	Exovesicle
T7633	29916-29926	NN	denotes	generation
T7634	29927-29931	IN	denotes	from
T7635	29932-29937	JJ	denotes	human
T7637	29938-29941	CC	denotes	and
T7638	29942-29948	JJ	denotes	murine
T7639	29949-29952	JJ	denotes	red
T7640	29953-29958	NN	denotes	blood
T7636	29959-29964	NNS	denotes	cells
T7641	29964-30034	sentence	denotes	Red blood cells naturally can form hemoglobin containing exovesicles.
T7642	29965-29968	JJ	denotes	Red
T7644	29969-29974	NN	denotes	blood
T7643	29975-29980	NNS	denotes	cells
T7646	29981-29990	RB	denotes	naturally
T7647	29991-29994	MD	denotes	can
T7645	29995-29999	VB	denotes	form
T7648	30000-30010	NN	denotes	hemoglobin
T7649	30011-30021	VBG	denotes	containing
T7650	30022-30033	NNS	denotes	exovesicles
T7651	30033-30034	.	denotes	.
T7652	30034-30084	sentence	denotes	In vitro these vesicle formation is Ca2+ induced.
T7653	30035-30037	FW	denotes	In
T7654	30038-30043	FW	denotes	vitro
T7656	30044-30049	DT	denotes	these
T7658	30050-30057	NN	denotes	vesicle
T7657	30058-30067	NN	denotes	formation
T7655	30068-30070	VBZ	denotes	is
T7659	30071-30075	NN	denotes	Ca2+
T7660	30076-30083	VBN	denotes	induced
T7661	30083-30084	.	denotes	.
T7662	30084-30198	sentence	denotes	Freshly collected lithium-heparin anticoagulated blood samples were centrifuged for 5 minutes at 400 × g and 4°C.
T7663	30085-30092	RB	denotes	Freshly
T7664	30093-30102	VBN	denotes	collected
T7666	30103-30110	NN	denotes	lithium
T7668	30110-30111	HYPH	denotes	-
T7667	30111-30118	NN	denotes	heparin
T7669	30119-30133	VBN	denotes	anticoagulated
T7670	30134-30139	NN	denotes	blood
T7665	30140-30147	NNS	denotes	samples
T7672	30148-30152	VBD	denotes	were
T7671	30153-30164	VBN	denotes	centrifuged
T7673	30165-30168	IN	denotes	for
T7674	30169-30170	CD	denotes	5
T7675	30171-30178	NNS	denotes	minutes
T7676	30179-30181	IN	denotes	at
T7677	30182-30185	CD	denotes	400
T7679	30186-30187	SYM	denotes	×
T7678	30188-30189	NN	denotes	g
T7680	30190-30193	CC	denotes	and
T7681	30194-30195	CD	denotes	4
T7682	30195-30197	NN	denotes	°C
T7683	30197-30198	.	denotes	.
T7684	30198-30298	sentence	denotes	The erythrocytes were washed five times with 20 mM Hepes, pH 7.5, and 150 mM NaCl (washing buffer).
T7685	30199-30202	DT	denotes	The
T7686	30203-30215	NNS	denotes	erythrocytes
T7688	30216-30220	VBD	denotes	were
T7687	30221-30227	VBN	denotes	washed
T7689	30228-30232	CD	denotes	five
T7690	30233-30238	NNS	denotes	times
T7691	30239-30243	IN	denotes	with
T7692	30244-30246	CD	denotes	20
T7693	30247-30249	NN	denotes	mM
T7694	30250-30255	NN	denotes	Hepes
T7695	30255-30257	,	denotes	,
T7696	30257-30259	NN	denotes	pH
T7697	30260-30263	CD	denotes	7.5
T7698	30263-30265	,	denotes	,
T7699	30265-30268	CC	denotes	and
T7700	30269-30272	CD	denotes	150
T7701	30273-30275	NN	denotes	mM
T7702	30276-30280	NN	denotes	NaCl
T7703	30281-30282	-LRB-	denotes	(
T7705	30282-30289	VBG	denotes	washing
T7704	30290-30296	NN	denotes	buffer
T7706	30296-30297	-RRB-	denotes	)
T7707	30297-30298	.	denotes	.
T7708	30298-30511	sentence	denotes	A volume of 500 μl washed red blood cells were combined with 3 ml of vesiculation buffer (20 mM Hepes, pH 7.5, 150 mM NaCl, 1 mM CaCl2 adjusted to 5 μM Ca2+ ionophore A23187) and incubated for 30 minutes at 37°C.
T7709	30299-30300	DT	denotes	A
T7710	30301-30307	NN	denotes	volume
T7712	30308-30310	IN	denotes	of
T7713	30311-30314	CD	denotes	500
T7714	30315-30317	NN	denotes	μl
T7716	30318-30324	VBN	denotes	washed
T7717	30325-30328	JJ	denotes	red
T7718	30329-30334	NN	denotes	blood
T7715	30335-30340	NNS	denotes	cells
T7719	30341-30345	VBD	denotes	were
T7711	30346-30354	VBN	denotes	combined
T7720	30355-30359	IN	denotes	with
T7721	30360-30361	CD	denotes	3
T7722	30362-30364	NN	denotes	ml
T7723	30365-30367	IN	denotes	of
T7724	30368-30380	NN	denotes	vesiculation
T7725	30381-30387	NN	denotes	buffer
T7726	30388-30389	-LRB-	denotes	(
T7728	30389-30391	CD	denotes	20
T7729	30392-30394	NN	denotes	mM
T7730	30395-30400	NN	denotes	Hepes
T7731	30400-30402	,	denotes	,
T7732	30402-30404	NN	denotes	pH
T7733	30405-30408	CD	denotes	7.5
T7734	30408-30410	,	denotes	,
T7735	30410-30413	CD	denotes	150
T7736	30414-30416	NN	denotes	mM
T7737	30417-30421	NN	denotes	NaCl
T7738	30421-30423	,	denotes	,
T7739	30423-30424	CD	denotes	1
T7740	30425-30427	NN	denotes	mM
T7727	30428-30433	NN	denotes	CaCl2
T7741	30434-30442	VBN	denotes	adjusted
T7742	30443-30445	IN	denotes	to
T7743	30446-30447	CD	denotes	5
T7744	30448-30450	NN	denotes	μM
T7746	30451-30455	NN	denotes	Ca2+
T7747	30456-30465	NN	denotes	ionophore
T7745	30466-30472	NN	denotes	A23187
T7748	30472-30473	-RRB-	denotes	)
T7749	30474-30477	CC	denotes	and
T7750	30478-30487	VBN	denotes	incubated
T7751	30488-30491	IN	denotes	for
T7752	30492-30494	CD	denotes	30
T7753	30495-30502	NNS	denotes	minutes
T7754	30503-30505	IN	denotes	at
T7755	30506-30508	CD	denotes	37
T7756	30508-30510	NNS	denotes	°C
T7757	30510-30511	.	denotes	.
T7758	30511-30642	sentence	denotes	After incubation EDTA was added to the solution to a final concentration of 5 mM and centrifuged for 5 minutes at 400 × g and 4°C.
T7759	30512-30517	IN	denotes	After
T7761	30518-30528	NN	denotes	incubation
T7762	30529-30533	NN	denotes	EDTA
T7763	30534-30537	VBD	denotes	was
T7760	30538-30543	VBN	denotes	added
T7764	30544-30546	IN	denotes	to
T7765	30547-30550	DT	denotes	the
T7766	30551-30559	NN	denotes	solution
T7767	30560-30562	IN	denotes	to
T7768	30563-30564	DT	denotes	a
T7770	30565-30570	JJ	denotes	final
T7769	30571-30584	NN	denotes	concentration
T7771	30585-30587	IN	denotes	of
T7772	30588-30589	CD	denotes	5
T7773	30590-30592	NN	denotes	mM
T7774	30593-30596	CC	denotes	and
T7775	30597-30608	VBN	denotes	centrifuged
T7776	30609-30612	IN	denotes	for
T7777	30613-30614	CD	denotes	5
T7778	30615-30622	NNS	denotes	minutes
T7779	30623-30625	IN	denotes	at
T7780	30626-30629	CD	denotes	400
T7782	30630-30631	SYM	denotes	×
T7781	30632-30633	NN	denotes	g
T7783	30634-30637	CC	denotes	and
T7784	30638-30639	CD	denotes	4
T7785	30639-30641	NNS	denotes	°C
T7786	30641-30642	.	denotes	.
T7787	30642-30726	sentence	denotes	The supernatant was collected and centrifuged for 20 minutes at 15,000 × g and 4°C.
T7788	30643-30646	DT	denotes	The
T7789	30647-30658	NN	denotes	supernatant
T7791	30659-30662	VBD	denotes	was
T7790	30663-30672	VBN	denotes	collected
T7792	30673-30676	CC	denotes	and
T7793	30677-30688	VBN	denotes	centrifuged
T7794	30689-30692	IN	denotes	for
T7795	30693-30695	CD	denotes	20
T7796	30696-30703	NNS	denotes	minutes
T7797	30704-30706	IN	denotes	at
T7798	30707-30713	CD	denotes	15,000
T7800	30714-30715	SYM	denotes	×
T7799	30716-30717	NN	denotes	g
T7801	30718-30721	CC	denotes	and
T7802	30722-30723	CD	denotes	4
T7803	30723-30725	NNS	denotes	°C
T7804	30725-30726	.	denotes	.
T7805	30726-30883	sentence	denotes	The resulting pellet was resuspended in washing buffer and centrifuged at 400 × g again to collect a supernatant enriched with red blood cell microvesicles.
T7806	30727-30730	DT	denotes	The
T7808	30731-30740	VBG	denotes	resulting
T7807	30741-30747	NN	denotes	pellet
T7810	30748-30751	VBD	denotes	was
T7809	30752-30763	VBN	denotes	resuspended
T7811	30764-30766	IN	denotes	in
T7812	30767-30774	VBG	denotes	washing
T7813	30775-30781	NN	denotes	buffer
T7814	30782-30785	CC	denotes	and
T7815	30786-30797	VBN	denotes	centrifuged
T7816	30798-30800	IN	denotes	at
T7817	30801-30804	CD	denotes	400
T7819	30805-30806	SYM	denotes	×
T7818	30807-30808	NN	denotes	g
T7820	30809-30814	RB	denotes	again
T7821	30815-30817	TO	denotes	to
T7822	30818-30825	VB	denotes	collect
T7823	30826-30827	DT	denotes	a
T7824	30828-30839	NN	denotes	supernatant
T7825	30840-30848	VBN	denotes	enriched
T7826	30849-30853	IN	denotes	with
T7827	30854-30857	JJ	denotes	red
T7829	30858-30863	NN	denotes	blood
T7828	30864-30868	NN	denotes	cell
T7830	30869-30882	NNS	denotes	microvesicles
T7831	30882-30883	.	denotes	.
T7832	30883-30985	sentence	denotes	The supernatant of the 15,000 × g step was further centrifuged for 60 minutes at 100,000 × g and 4°C.
T7833	30884-30887	DT	denotes	The
T7834	30888-30899	NN	denotes	supernatant
T7836	30900-30902	IN	denotes	of
T7837	30903-30906	DT	denotes	the
T7839	30907-30913	CD	denotes	15,000
T7841	30914-30915	SYM	denotes	×
T7840	30916-30917	NN	denotes	g
T7838	30918-30922	NN	denotes	step
T7842	30923-30926	VBD	denotes	was
T7843	30927-30934	RBR	denotes	further
T7835	30935-30946	VBN	denotes	centrifuged
T7844	30947-30950	IN	denotes	for
T7845	30951-30953	CD	denotes	60
T7846	30954-30961	NNS	denotes	minutes
T7847	30962-30964	IN	denotes	at
T7848	30965-30972	CD	denotes	100,000
T7850	30973-30974	SYM	denotes	×
T7849	30975-30976	NN	denotes	g
T7851	30977-30980	CC	denotes	and
T7852	30981-30982	CD	denotes	4
T7853	30982-30984	NNS	denotes	°C
T7854	30984-30985	.	denotes	.
T7855	30985-31155	sentence	denotes	The final pellet of this step was resuspended in washing buffer and centrifuged at 15,000 × g again to purify a supernatant enriched in nanovesicles according to [7,20].
T7856	30986-30989	DT	denotes	The
T7858	30990-30995	JJ	denotes	final
T7857	30996-31002	NN	denotes	pellet
T7860	31003-31005	IN	denotes	of
T7861	31006-31010	DT	denotes	this
T7862	31011-31015	NN	denotes	step
T7863	31016-31019	VBD	denotes	was
T7859	31020-31031	VBN	denotes	resuspended
T7864	31032-31034	IN	denotes	in
T7865	31035-31042	VBG	denotes	washing
T7866	31043-31049	NN	denotes	buffer
T7867	31050-31053	CC	denotes	and
T7868	31054-31065	VBN	denotes	centrifuged
T7869	31066-31068	IN	denotes	at
T7870	31069-31075	CD	denotes	15,000
T7872	31076-31077	SYM	denotes	×
T7871	31078-31079	NN	denotes	g
T7873	31080-31085	RB	denotes	again
T7874	31086-31088	TO	denotes	to
T7875	31089-31095	VB	denotes	purify
T7876	31096-31097	DT	denotes	a
T7877	31098-31109	NN	denotes	supernatant
T7878	31110-31118	VBN	denotes	enriched
T7879	31119-31121	IN	denotes	in
T7880	31122-31134	NNS	denotes	nanovesicles
T7881	31135-31144	VBG	denotes	according
T7882	31145-31147	IN	denotes	to
T7883	31148-31149	-LRB-	denotes	[
T7884	31149-31150	CD	denotes	7
T7886	31150-31151	,	denotes	,
T7885	31151-31153	CD	denotes	20
T7887	31153-31154	-RRB-	denotes	]
T7888	31154-31155	.	denotes	.
T7889	31155-31248	sentence	denotes	The vesicles were assayed for acetylcholine esterase activity and used for further analysis.
T7890	31156-31159	DT	denotes	The
T7891	31160-31168	NNS	denotes	vesicles
T7893	31169-31173	VBD	denotes	were
T7892	31174-31181	VBN	denotes	assayed
T7894	31182-31185	IN	denotes	for
T7895	31186-31199	NN	denotes	acetylcholine
T7896	31200-31208	NN	denotes	esterase
T7897	31209-31217	NN	denotes	activity
T7898	31218-31221	CC	denotes	and
T7899	31222-31226	VBN	denotes	used
T7900	31227-31230	IN	denotes	for
T7901	31231-31238	JJ	denotes	further
T7902	31239-31247	NN	denotes	analysis
T7903	31247-31248	.	denotes	.
T7951	31250-31263	NN	denotes	Acetylcholine
T7952	31264-31272	NN	denotes	esterase
T7953	31273-31278	NN	denotes	assay
T7954	31278-31463	sentence	denotes	A 30 μl sample of red blood cell vesicles was mixed with an equal volume of buffer (20 mM Hepes, pH 7.6, 150 mM NaCl, 0.5 % Triton X-100), vortexed and incubated for 5 minutes at 37°C.
T7955	31279-31280	DT	denotes	A
T7957	31281-31283	CD	denotes	30
T7958	31284-31286	NN	denotes	μl
T7956	31287-31293	NN	denotes	sample
T7960	31294-31296	IN	denotes	of
T7961	31297-31300	JJ	denotes	red
T7963	31301-31306	NN	denotes	blood
T7962	31307-31311	NN	denotes	cell
T7964	31312-31320	NNS	denotes	vesicles
T7965	31321-31324	VBD	denotes	was
T7959	31325-31330	VBN	denotes	mixed
T7966	31331-31335	IN	denotes	with
T7967	31336-31338	DT	denotes	an
T7969	31339-31344	JJ	denotes	equal
T7968	31345-31351	NN	denotes	volume
T7970	31352-31354	IN	denotes	of
T7971	31355-31361	NN	denotes	buffer
T7972	31362-31363	-LRB-	denotes	(
T7974	31363-31365	CD	denotes	20
T7975	31366-31368	NN	denotes	mM
T7976	31369-31374	NN	denotes	Hepes
T7977	31374-31376	,	denotes	,
T7978	31376-31378	NN	denotes	pH
T7979	31379-31382	CD	denotes	7.6
T7980	31382-31384	,	denotes	,
T7981	31384-31387	CD	denotes	150
T7982	31388-31390	NN	denotes	mM
T7983	31391-31395	NN	denotes	NaCl
T7984	31395-31397	,	denotes	,
T7985	31397-31400	CD	denotes	0.5
T7986	31401-31402	NN	denotes	%
T7987	31403-31409	NN	denotes	Triton
T7973	31410-31411	NN	denotes	X
T7988	31411-31412	HYPH	denotes	-
T7989	31412-31415	CD	denotes	100
T7990	31415-31416	-RRB-	denotes	)
T7991	31416-31418	,	denotes	,
T7992	31418-31426	VBN	denotes	vortexed
T7993	31427-31430	CC	denotes	and
T7994	31431-31440	VBN	denotes	incubated
T7995	31441-31444	IN	denotes	for
T7996	31445-31446	CD	denotes	5
T7997	31447-31454	NNS	denotes	minutes
T7998	31455-31457	IN	denotes	at
T7999	31458-31460	CD	denotes	37
T8000	31460-31462	NNS	denotes	°C
T8001	31462-31463	.	denotes	.
T8002	31463-31683	sentence	denotes	Subsequently 640 μl sodium phosphate buffer (100 mM Na2PO4, pH 7.6), 50 μl DTNB (5,5'-dithiobis-(2-nitrobenzoic acid), 10 mM in sodium phosphate buffer), and 50 μl acetylthiocholine chloride (12,5 mM in H2O) were added.
T8003	31464-31476	RB	denotes	Subsequently
T8005	31477-31480	CD	denotes	640
T8006	31481-31483	NN	denotes	μl
T8008	31484-31490	NN	denotes	sodium
T8009	31491-31500	NN	denotes	phosphate
T8007	31501-31507	NN	denotes	buffer
T8010	31508-31509	-LRB-	denotes	(
T8012	31509-31512	CD	denotes	100
T8013	31513-31515	NN	denotes	mM
T8011	31516-31522	NN	denotes	Na2PO4
T8014	31522-31524	,	denotes	,
T8015	31524-31526	NN	denotes	pH
T8016	31527-31530	CD	denotes	7.6
T8017	31530-31531	-RRB-	denotes	)
T8018	31531-31533	,	denotes	,
T8019	31533-31535	CD	denotes	50
T8020	31536-31538	NN	denotes	μl
T8021	31539-31543	NN	denotes	DTNB
T8022	31544-31545	-LRB-	denotes	(
T8024	31545-31546	CD	denotes	5
T8026	31546-31547	,	denotes	,
T8027	31547-31548	CD	denotes	5
T8028	31548-31549	SYM	denotes	'
T8029	31549-31550	HYPH	denotes	-
T8025	31550-31559	NN	denotes	dithiobis
T8030	31559-31560	HYPH	denotes	-
T8031	31560-31561	-LRB-	denotes	(
T8033	31561-31562	CD	denotes	2
T8034	31562-31563	HYPH	denotes	-
T8035	31563-31575	JJ	denotes	nitrobenzoic
T8032	31576-31580	NN	denotes	acid
T8036	31580-31581	-RRB-	denotes	)
T8037	31581-31583	,	denotes	,
T8038	31583-31585	CD	denotes	10
T8023	31586-31588	NN	denotes	mM
T8039	31589-31591	IN	denotes	in
T8040	31592-31598	NN	denotes	sodium
T8041	31599-31608	NN	denotes	phosphate
T8042	31609-31615	NN	denotes	buffer
T8043	31615-31616	-RRB-	denotes	)
T8044	31616-31618	,	denotes	,
T8045	31618-31621	CC	denotes	and
T8046	31622-31624	CD	denotes	50
T8047	31625-31627	NN	denotes	μl
T8049	31628-31645	NN	denotes	acetylthiocholine
T8048	31646-31654	NN	denotes	chloride
T8050	31655-31656	-LRB-	denotes	(
T8052	31656-31658	CD	denotes	12
T8053	31658-31659	,	denotes	,
T8054	31659-31660	CD	denotes	5
T8051	31661-31663	NN	denotes	mM
T8055	31664-31666	IN	denotes	in
T8056	31667-31670	NN	denotes	H2O
T8057	31670-31671	-RRB-	denotes	)
T8058	31672-31676	VBD	denotes	were
T8004	31677-31682	VBN	denotes	added
T8059	31682-31683	.	denotes	.
T8060	31683-31825	sentence	denotes	The reaction took place at room temperature and was measured photometrically at a wavelength of 405 nm according to the Ellmann' method [25].
T8061	31684-31687	DT	denotes	The
T8062	31688-31696	NN	denotes	reaction
T8063	31697-31701	VBD	denotes	took
T8064	31702-31707	NN	denotes	place
T8065	31708-31710	IN	denotes	at
T8066	31711-31715	NN	denotes	room
T8067	31716-31727	NN	denotes	temperature
T8068	31728-31731	CC	denotes	and
T8069	31732-31735	VBD	denotes	was
T8070	31736-31744	VBN	denotes	measured
T8071	31745-31760	RB	denotes	photometrically
T8072	31761-31763	IN	denotes	at
T8073	31764-31765	DT	denotes	a
T8074	31766-31776	NN	denotes	wavelength
T8075	31777-31779	IN	denotes	of
T8076	31780-31783	CD	denotes	405
T8077	31784-31786	NNS	denotes	nm
T8078	31787-31796	VBG	denotes	according
T8079	31797-31799	IN	denotes	to
T8080	31800-31803	DT	denotes	the
T8082	31804-31811	NNP	denotes	Ellmann
T8083	31811-31812	POS	denotes	'
T8081	31813-31819	NN	denotes	method
T8084	31820-31821	-LRB-	denotes	[
T8085	31821-31823	CD	denotes	25
T8086	31823-31824	-RRB-	denotes	]
T8087	31824-31825	.	denotes	.
T8113	31827-31840	NN	denotes	Determination
T8114	31841-31843	IN	denotes	of
T8115	31844-31847	DT	denotes	the
T8117	31848-31855	JJ	denotes	osmotic
T8116	31856-31866	NN	denotes	resistance
T8118	31867-31869	IN	denotes	of
T8119	31870-31873	JJ	denotes	red
T8121	31874-31879	NN	denotes	blood
T8120	31880-31885	NNS	denotes	cells
T8122	31885-32068	sentence	denotes	A Na2HPO4/NaH2PO4 buffered NaCl gradient (pH 7.4) comprised of the following NaCl steps was used for this assay: 0.90%, 0.65%, 0.60%, 0.55%, 0.50%, 0.45%, 0.40%, 0.35%, 0.30%, 0.10%.
T8123	31886-31887	DT	denotes	A
T8125	31888-31895	NN	denotes	Na2HPO4
T8127	31895-31896	HYPH	denotes	/
T8126	31896-31903	NN	denotes	NaH2PO4
T8128	31904-31912	VBN	denotes	buffered
T8129	31913-31917	NN	denotes	NaCl
T8124	31918-31926	NN	denotes	gradient
T8131	31927-31928	-LRB-	denotes	(
T8132	31928-31930	NN	denotes	pH
T8133	31931-31934	CD	denotes	7.4
T8134	31934-31935	-RRB-	denotes	)
T8135	31936-31945	VBN	denotes	comprised
T8136	31946-31948	IN	denotes	of
T8137	31949-31952	DT	denotes	the
T8139	31953-31962	VBG	denotes	following
T8140	31963-31967	NN	denotes	NaCl
T8138	31968-31973	NNS	denotes	steps
T8141	31974-31977	VBD	denotes	was
T8130	31978-31982	VBN	denotes	used
T8142	31983-31986	IN	denotes	for
T8143	31987-31991	DT	denotes	this
T8144	31992-31997	NN	denotes	assay
T8145	31997-31999	:	denotes	:
T8146	31999-32003	CD	denotes	0.90
T8147	32003-32004	NN	denotes	%
T8148	32004-32006	,	denotes	,
T8149	32006-32010	CD	denotes	0.65
T8150	32010-32011	NN	denotes	%
T8151	32011-32013	,	denotes	,
T8152	32013-32017	CD	denotes	0.60
T8153	32017-32018	NN	denotes	%
T8154	32018-32020	,	denotes	,
T8155	32020-32024	CD	denotes	0.55
T8156	32024-32025	NN	denotes	%
T8157	32025-32027	,	denotes	,
T8158	32027-32031	CD	denotes	0.50
T8159	32031-32032	NN	denotes	%
T8160	32032-32034	,	denotes	,
T8161	32034-32038	CD	denotes	0.45
T8162	32038-32039	NN	denotes	%
T8163	32039-32041	,	denotes	,
T8164	32041-32045	CD	denotes	0.40
T8165	32045-32046	NN	denotes	%
T8166	32046-32048	,	denotes	,
T8167	32048-32052	CD	denotes	0.35
T8168	32052-32053	NN	denotes	%
T8169	32053-32055	,	denotes	,
T8170	32055-32059	CD	denotes	0.30
T8171	32059-32060	NN	denotes	%
T8172	32060-32062	,	denotes	,
T8173	32062-32066	CD	denotes	0.10
T8174	32066-32067	NN	denotes	%
T8175	32067-32068	.	denotes	.
T8176	32068-32217	sentence	denotes	For each step 5 ml of the gradient step solution and 50 μl blood sample were carefully inverted four times and incubated for 30 minutes in the dark.
T8177	32069-32072	IN	denotes	For
T8179	32073-32077	DT	denotes	each
T8180	32078-32082	NN	denotes	step
T8181	32083-32084	CD	denotes	5
T8182	32085-32087	NNS	denotes	ml
T8183	32088-32090	IN	denotes	of
T8184	32091-32094	DT	denotes	the
T8186	32095-32103	NN	denotes	gradient
T8187	32104-32108	NN	denotes	step
T8185	32109-32117	NN	denotes	solution
T8188	32118-32121	CC	denotes	and
T8189	32122-32124	CD	denotes	50
T8190	32125-32127	NN	denotes	μl
T8192	32128-32133	NN	denotes	blood
T8191	32134-32140	NN	denotes	sample
T8193	32141-32145	VBD	denotes	were
T8194	32146-32155	RB	denotes	carefully
T8178	32156-32164	VBN	denotes	inverted
T8195	32165-32169	CD	denotes	four
T8196	32170-32175	NNS	denotes	times
T8197	32176-32179	CC	denotes	and
T8198	32180-32189	VBN	denotes	incubated
T8199	32190-32193	IN	denotes	for
T8200	32194-32196	CD	denotes	30
T8201	32197-32204	NNS	denotes	minutes
T8202	32205-32207	IN	denotes	in
T8203	32208-32211	DT	denotes	the
T8204	32212-32216	NN	denotes	dark
T8205	32216-32217	.	denotes	.
T8206	32217-32300	sentence	denotes	Samples were carefully inverted again and centrifuged at 1,500 × g for 10 minutes.
T8207	32218-32225	NNS	denotes	Samples
T8209	32226-32230	VBD	denotes	were
T8210	32231-32240	RB	denotes	carefully
T8208	32241-32249	VBN	denotes	inverted
T8211	32250-32255	RB	denotes	again
T8212	32256-32259	CC	denotes	and
T8213	32260-32271	VBN	denotes	centrifuged
T8214	32272-32274	IN	denotes	at
T8215	32275-32280	CD	denotes	1,500
T8217	32281-32282	SYM	denotes	×
T8216	32283-32284	NN	denotes	g
T8218	32285-32288	IN	denotes	for
T8219	32289-32291	CD	denotes	10
T8220	32292-32299	NNS	denotes	minutes
T8221	32299-32300	.	denotes	.
T8222	32300-32385	sentence	denotes	From 1 ml supernatant of each step the extinction at 546 nm wavelength was measured.
T8223	32301-32305	IN	denotes	From
T8225	32306-32307	CD	denotes	1
T8226	32308-32310	NN	denotes	ml
T8227	32311-32322	NN	denotes	supernatant
T8228	32323-32325	IN	denotes	of
T8229	32326-32330	DT	denotes	each
T8230	32331-32335	NN	denotes	step
T8231	32336-32339	DT	denotes	the
T8232	32340-32350	NN	denotes	extinction
T8233	32351-32353	IN	denotes	at
T8234	32354-32357	CD	denotes	546
T8235	32358-32360	NN	denotes	nm
T8236	32361-32371	NN	denotes	wavelength
T8237	32372-32375	VBD	denotes	was
T8224	32376-32384	VBN	denotes	measured
T8238	32384-32385	.	denotes	.
T8239	32385-32435	sentence	denotes	The experiment was performed at room temperature.
T8240	32386-32389	DT	denotes	The
T8241	32390-32400	NN	denotes	experiment
T8243	32401-32404	VBD	denotes	was
T8242	32405-32414	VBN	denotes	performed
T8244	32415-32417	IN	denotes	at
T8245	32418-32422	NN	denotes	room
T8246	32423-32434	NN	denotes	temperature
T8247	32434-32435	.	denotes	.
T8326	32437-32445	NN	denotes	Platelet
T8327	32446-32457	NN	denotes	aggregation
T8328	32458-32463	NN	denotes	assay
T8329	32463-32544	sentence	denotes	Approximately 500 μl of murine blood were centrifuged at 200 × g for 15 minutes.
T8330	32464-32477	RB	denotes	Approximately
T8331	32478-32481	CD	denotes	500
T8332	32482-32484	NNS	denotes	μl
T8334	32485-32487	IN	denotes	of
T8335	32488-32494	JJ	denotes	murine
T8336	32495-32500	NN	denotes	blood
T8337	32501-32505	VBD	denotes	were
T8333	32506-32517	VBN	denotes	centrifuged
T8338	32518-32520	IN	denotes	at
T8339	32521-32524	CD	denotes	200
T8341	32525-32526	SYM	denotes	×
T8340	32527-32528	NN	denotes	g
T8342	32529-32532	IN	denotes	for
T8343	32533-32535	CD	denotes	15
T8344	32536-32543	NNS	denotes	minutes
T8345	32543-32544	.	denotes	.
T8346	32544-32641	sentence	denotes	The resulting supernatants were transferred to new tubes and used as platelet rich plasma (PRP).
T8347	32545-32548	DT	denotes	The
T8349	32549-32558	VBG	denotes	resulting
T8348	32559-32571	NNS	denotes	supernatants
T8351	32572-32576	VBD	denotes	were
T8350	32577-32588	VBN	denotes	transferred
T8352	32589-32591	IN	denotes	to
T8353	32592-32595	JJ	denotes	new
T8354	32596-32601	NNS	denotes	tubes
T8355	32602-32605	CC	denotes	and
T8356	32606-32610	VBN	denotes	used
T8357	32611-32613	IN	denotes	as
T8358	32614-32622	NN	denotes	platelet
T8359	32623-32627	JJ	denotes	rich
T8360	32628-32634	NN	denotes	plasma
T8361	32635-32636	-LRB-	denotes	(
T8362	32636-32639	NN	denotes	PRP
T8363	32639-32640	-RRB-	denotes	)
T8364	32640-32641	.	denotes	.
T8365	32641-32834	sentence	denotes	The platelet aggregation measurements were done according to the method developed by Born and Cross [26] using an APACT photometer (Labor, Ahrendsburg, FRG) and the APACT software (APACT 1.4).
T8366	32642-32645	DT	denotes	The
T8368	32646-32654	NN	denotes	platelet
T8369	32655-32666	NN	denotes	aggregation
T8367	32667-32679	NNS	denotes	measurements
T8371	32680-32684	VBD	denotes	were
T8370	32685-32689	VBN	denotes	done
T8372	32690-32699	VBG	denotes	according
T8373	32700-32702	IN	denotes	to
T8374	32703-32706	DT	denotes	the
T8375	32707-32713	NN	denotes	method
T8376	32714-32723	VBN	denotes	developed
T8377	32724-32726	IN	denotes	by
T8378	32727-32731	NNP	denotes	Born
T8379	32732-32735	CC	denotes	and
T8380	32736-32741	NNP	denotes	Cross
T8381	32742-32743	-LRB-	denotes	[
T8382	32743-32745	CD	denotes	26
T8383	32745-32746	-RRB-	denotes	]
T8384	32747-32752	VBG	denotes	using
T8385	32753-32755	DT	denotes	an
T8387	32756-32761	NN	denotes	APACT
T8386	32762-32772	NN	denotes	photometer
T8388	32773-32774	-LRB-	denotes	(
T8390	32774-32779	NN	denotes	Labor
T8391	32779-32781	,	denotes	,
T8392	32781-32792	NN	denotes	Ahrendsburg
T8393	32792-32794	,	denotes	,
T8389	32794-32797	NN	denotes	FRG
T8394	32797-32798	-RRB-	denotes	)
T8395	32799-32802	CC	denotes	and
T8396	32803-32806	DT	denotes	the
T8398	32807-32812	NN	denotes	APACT
T8397	32813-32821	NN	denotes	software
T8399	32822-32823	-LRB-	denotes	(
T8400	32823-32828	NN	denotes	APACT
T8401	32829-32832	CD	denotes	1.4
T8402	32832-32833	-RRB-	denotes	)
T8403	32833-32834	.	denotes	.
T8404	32834-32951	sentence	denotes	Subsequently samples of the PRP were adjusted with buffer (5 mM Hepes, 150 mM NaCl, pH 7.3) to 250,000 platelets/μl.
T8405	32835-32847	RB	denotes	Subsequently
T8407	32848-32855	NNS	denotes	samples
T8408	32856-32858	IN	denotes	of
T8409	32859-32862	DT	denotes	the
T8410	32863-32866	NN	denotes	PRP
T8411	32867-32871	VBD	denotes	were
T8406	32872-32880	VBN	denotes	adjusted
T8412	32881-32885	IN	denotes	with
T8413	32886-32892	NN	denotes	buffer
T8414	32893-32894	-LRB-	denotes	(
T8416	32894-32895	CD	denotes	5
T8417	32896-32898	NN	denotes	mM
T8418	32899-32904	NN	denotes	Hepes
T8419	32904-32906	,	denotes	,
T8420	32906-32909	CD	denotes	150
T8421	32910-32912	NN	denotes	mM
T8422	32913-32917	NN	denotes	NaCl
T8423	32917-32919	,	denotes	,
T8415	32919-32921	NN	denotes	pH
T8424	32922-32925	CD	denotes	7.3
T8425	32925-32926	-RRB-	denotes	)
T8426	32927-32929	IN	denotes	to
T8427	32930-32937	CD	denotes	250,000
T8428	32938-32947	NNS	denotes	platelets
T8429	32947-32948	SYM	denotes	/
T8430	32948-32950	NN	denotes	μl
T8431	32950-32951	.	denotes	.
T8432	32951-33076	sentence	denotes	A constant platelet count was used throughout allowing comparisons between wild type and knock out murine and human samples.
T8433	32952-32953	DT	denotes	A
T8435	32954-32962	JJ	denotes	constant
T8436	32963-32971	NN	denotes	platelet
T8434	32972-32977	NN	denotes	count
T8438	32978-32981	VBD	denotes	was
T8437	32982-32986	VBN	denotes	used
T8439	32987-32997	RB	denotes	throughout
T8440	32998-33006	VBG	denotes	allowing
T8441	33007-33018	NNS	denotes	comparisons
T8442	33019-33026	IN	denotes	between
T8443	33027-33031	JJ	denotes	wild
T8444	33032-33036	NN	denotes	type
T8446	33037-33040	CC	denotes	and
T8447	33041-33046	NN	denotes	knock
T8448	33047-33050	NN	denotes	out
T8449	33051-33057	JJ	denotes	murine
T8450	33058-33061	CC	denotes	and
T8451	33062-33067	JJ	denotes	human
T8445	33068-33075	NNS	denotes	samples
T8452	33075-33076	.	denotes	.
T8453	33076-33403	sentence	denotes	250 μl adjusted platelets were prewarmed to 37°C and used in the assay, and the aggregation process was started by adding 25 μl of a prewarmed 16.5 mg/ml ristocetin solution (DiaMed Diagnostica, Bensheim, FRG; concentration in the assay: 1.5 mg/ml), a reagent which worked reliably in our hands with mouse and human platelets.
T8454	33077-33080	CD	denotes	250
T8455	33081-33083	NN	denotes	μl
T8457	33084-33092	VBN	denotes	adjusted
T8456	33093-33102	NNS	denotes	platelets
T8459	33103-33107	VBD	denotes	were
T8458	33108-33117	VBN	denotes	prewarmed
T8460	33118-33120	IN	denotes	to
T8461	33121-33123	CD	denotes	37
T8462	33123-33125	NNS	denotes	°C
T8463	33126-33129	CC	denotes	and
T8464	33130-33134	VBN	denotes	used
T8465	33135-33137	IN	denotes	in
T8466	33138-33141	DT	denotes	the
T8467	33142-33147	NN	denotes	assay
T8468	33147-33149	,	denotes	,
T8469	33149-33152	CC	denotes	and
T8470	33153-33156	DT	denotes	the
T8472	33157-33168	NN	denotes	aggregation
T8471	33169-33176	NN	denotes	process
T8474	33177-33180	VBD	denotes	was
T8473	33181-33188	VBN	denotes	started
T8475	33189-33191	IN	denotes	by
T8476	33192-33198	VBG	denotes	adding
T8477	33199-33201	CD	denotes	25
T8478	33202-33204	NN	denotes	μl
T8479	33205-33207	IN	denotes	of
T8480	33208-33209	DT	denotes	a
T8482	33210-33219	JJ	denotes	prewarmed
T8483	33220-33224	CD	denotes	16.5
T8484	33225-33227	NN	denotes	mg
T8485	33227-33228	SYM	denotes	/
T8486	33228-33230	NN	denotes	ml
T8487	33231-33241	NN	denotes	ristocetin
T8481	33242-33250	NN	denotes	solution
T8488	33251-33252	-LRB-	denotes	(
T8490	33252-33258	NNP	denotes	DiaMed
T8491	33259-33270	NNP	denotes	Diagnostica
T8492	33270-33272	,	denotes	,
T8493	33272-33280	NNP	denotes	Bensheim
T8494	33280-33282	,	denotes	,
T8489	33282-33285	NNP	denotes	FRG
T8495	33285-33286	:	denotes	;
T8496	33287-33300	NN	denotes	concentration
T8498	33301-33303	IN	denotes	in
T8499	33304-33307	DT	denotes	the
T8500	33308-33313	NN	denotes	assay
T8501	33313-33315	:	denotes	:
T8502	33315-33318	CD	denotes	1.5
T8497	33319-33321	NNS	denotes	mg
T8503	33321-33322	SYM	denotes	/
T8504	33322-33324	NN	denotes	ml
T8505	33324-33325	-RRB-	denotes	)
T8506	33325-33327	,	denotes	,
T8507	33327-33328	DT	denotes	a
T8508	33329-33336	NN	denotes	reagent
T8509	33337-33342	WDT	denotes	which
T8510	33343-33349	VBD	denotes	worked
T8511	33350-33358	RB	denotes	reliably
T8512	33359-33361	IN	denotes	in
T8513	33362-33365	PRP$	denotes	our
T8514	33366-33371	NNS	denotes	hands
T8515	33372-33376	IN	denotes	with
T8516	33377-33382	NN	denotes	mouse
T8518	33383-33386	CC	denotes	and
T8519	33387-33392	JJ	denotes	human
T8517	33393-33402	NNS	denotes	platelets
T8520	33402-33403	.	denotes	.
T8521	33403-33452	sentence	denotes	Each murine assay represents a different animal.
T8522	33404-33408	DT	denotes	Each
T8524	33409-33415	JJ	denotes	murine
T8523	33416-33421	NN	denotes	assay
T8525	33422-33432	VBZ	denotes	represents
T8526	33433-33434	DT	denotes	a
T8528	33435-33444	JJ	denotes	different
T8527	33445-33451	NN	denotes	animal
T8529	33451-33452	.	denotes	.
T8530	33452-33544	sentence	denotes	Analyses of human blood samples were performed as described in the manufacturer's protocol.
T8531	33453-33461	NNS	denotes	Analyses
T8533	33462-33464	IN	denotes	of
T8534	33465-33470	JJ	denotes	human
T8536	33471-33476	NN	denotes	blood
T8535	33477-33484	NNS	denotes	samples
T8537	33485-33489	VBD	denotes	were
T8532	33490-33499	VBN	denotes	performed
T8538	33500-33502	IN	denotes	as
T8539	33503-33512	VBN	denotes	described
T8540	33513-33515	IN	denotes	in
T8541	33516-33519	DT	denotes	the
T8542	33520-33532	NN	denotes	manufacturer
T8544	33532-33534	POS	denotes	's
T8543	33535-33543	NN	denotes	protocol
T8545	33543-33544	.	denotes	.
T8546	33544-33637	sentence	denotes	Several repeated measurements were done per human individual to control the reproducibility.
T8547	33545-33552	JJ	denotes	Several
T8549	33553-33561	JJ	denotes	repeated
T8548	33562-33574	NNS	denotes	measurements
T8551	33575-33579	VBD	denotes	were
T8550	33580-33584	VBN	denotes	done
T8552	33585-33588	IN	denotes	per
T8553	33589-33594	JJ	denotes	human
T8554	33595-33605	NN	denotes	individual
T8555	33606-33608	TO	denotes	to
T8556	33609-33616	VB	denotes	control
T8557	33617-33620	DT	denotes	the
T8558	33621-33636	NN	denotes	reproducibility
T8559	33636-33637	.	denotes	.
T8560	33637-33840	sentence	denotes	The acquired aggregation curve data, measured as ascending degree of transmission, were analysed for maximal slope values, while the maximal aggregation amplitude of every single curve was set to 100 %.
T8561	33638-33641	DT	denotes	The
T8563	33642-33650	VBN	denotes	acquired
T8564	33651-33662	NN	denotes	aggregation
T8565	33663-33668	NN	denotes	curve
T8562	33669-33673	NNS	denotes	data
T8567	33673-33675	,	denotes	,
T8568	33675-33683	VBN	denotes	measured
T8569	33684-33686	IN	denotes	as
T8570	33687-33696	VBG	denotes	ascending
T8571	33697-33703	NN	denotes	degree
T8572	33704-33706	IN	denotes	of
T8573	33707-33719	NN	denotes	transmission
T8574	33719-33721	,	denotes	,
T8575	33721-33725	VBD	denotes	were
T8566	33726-33734	VBN	denotes	analysed
T8576	33735-33738	IN	denotes	for
T8577	33739-33746	JJ	denotes	maximal
T8579	33747-33752	NN	denotes	slope
T8578	33753-33759	NNS	denotes	values
T8580	33759-33761	,	denotes	,
T8581	33761-33766	IN	denotes	while
T8583	33767-33770	DT	denotes	the
T8585	33771-33778	JJ	denotes	maximal
T8586	33779-33790	NN	denotes	aggregation
T8584	33791-33800	NN	denotes	amplitude
T8587	33801-33803	IN	denotes	of
T8588	33804-33809	DT	denotes	every
T8590	33810-33816	JJ	denotes	single
T8589	33817-33822	NN	denotes	curve
T8591	33823-33826	VBD	denotes	was
T8582	33827-33830	VBN	denotes	set
T8592	33831-33833	IN	denotes	to
T8593	33834-33837	CD	denotes	100
T8594	33838-33839	NN	denotes	%
T8595	33839-33840	.	denotes	.
T8596	33840-33947	sentence	denotes	In general, all platelet procedures were done at 22°C within four hours after collecting the blood sample.
T8597	33841-33843	IN	denotes	In
T8599	33844-33851	JJ	denotes	general
T8600	33851-33853	,	denotes	,
T8601	33853-33856	DT	denotes	all
T8603	33857-33865	NN	denotes	platelet
T8602	33866-33876	NNS	denotes	procedures
T8604	33877-33881	VBD	denotes	were
T8598	33882-33886	VBN	denotes	done
T8605	33887-33889	IN	denotes	at
T8606	33890-33892	CD	denotes	22
T8607	33892-33894	NNS	denotes	°C
T8608	33895-33901	IN	denotes	within
T8609	33902-33906	CD	denotes	four
T8610	33907-33912	NNS	denotes	hours
T8611	33913-33918	IN	denotes	after
T8612	33919-33929	VBG	denotes	collecting
T8613	33930-33933	DT	denotes	the
T8615	33934-33939	NN	denotes	blood
T8614	33940-33946	NN	denotes	sample
T8616	33946-33947	.	denotes	.
T8735	33949-33962	JJ	denotes	Miscellaneous
T8736	33963-33970	NNS	denotes	methods
T8737	33970-34147	sentence	denotes	For standard haematopoetic measurements and cell counts on murine and human blood samples about 300 μl of blood were analysed with an ADVIA 120 electronic cell counter (Bayer).
T8738	33971-33974	IN	denotes	For
T8740	33975-33983	JJ	denotes	standard
T8742	33984-33997	JJ	denotes	haematopoetic
T8741	33998-34010	NNS	denotes	measurements
T8743	34011-34014	CC	denotes	and
T8744	34015-34019	NN	denotes	cell
T8745	34020-34026	NNS	denotes	counts
T8746	34027-34029	IN	denotes	on
T8747	34030-34036	JJ	denotes	murine
T8749	34037-34040	CC	denotes	and
T8750	34041-34046	JJ	denotes	human
T8751	34047-34052	NN	denotes	blood
T8748	34053-34060	NNS	denotes	samples
T8752	34061-34066	IN	denotes	about
T8753	34067-34070	CD	denotes	300
T8754	34071-34073	NNS	denotes	μl
T8755	34074-34076	IN	denotes	of
T8756	34077-34082	NN	denotes	blood
T8757	34083-34087	VBD	denotes	were
T8739	34088-34096	VBN	denotes	analysed
T8758	34097-34101	IN	denotes	with
T8759	34102-34104	DT	denotes	an
T8761	34105-34110	NN	denotes	ADVIA
T8762	34111-34114	CD	denotes	120
T8763	34115-34125	JJ	denotes	electronic
T8764	34126-34130	NN	denotes	cell
T8760	34131-34138	NN	denotes	counter
T8765	34139-34140	-LRB-	denotes	(
T8766	34140-34145	NN	denotes	Bayer
T8767	34145-34146	-RRB-	denotes	)
T8768	34146-34147	.	denotes	.
T8769	34147-34268	sentence	denotes	With this equipment red blood cells and platelets are "transformed" into spherical bodies without changing their volume.
T8770	34148-34152	IN	denotes	With
T8772	34153-34157	DT	denotes	this
T8773	34158-34167	NN	denotes	equipment
T8774	34168-34171	JJ	denotes	red
T8776	34172-34177	NN	denotes	blood
T8775	34178-34183	NNS	denotes	cells
T8777	34184-34187	CC	denotes	and
T8778	34188-34197	NNS	denotes	platelets
T8779	34198-34201	VBP	denotes	are
T8780	34202-34203	``	denotes	"
T8771	34203-34214	VBN	denotes	transformed
T8781	34214-34215	''	denotes	"
T8782	34216-34220	IN	denotes	into
T8783	34221-34230	JJ	denotes	spherical
T8784	34231-34237	NNS	denotes	bodies
T8785	34238-34245	IN	denotes	without
T8786	34246-34254	VBG	denotes	changing
T8787	34255-34260	PRP$	denotes	their
T8788	34261-34267	NN	denotes	volume
T8789	34267-34268	.	denotes	.
T8790	34268-34386	sentence	denotes	Therefore number and volume of red blood cells and platelets are not calculated values, but directly measured values.
T8791	34269-34278	RB	denotes	Therefore
T8793	34279-34285	NN	denotes	number
T8794	34286-34289	CC	denotes	and
T8795	34290-34296	NN	denotes	volume
T8796	34297-34299	IN	denotes	of
T8797	34300-34303	JJ	denotes	red
T8799	34304-34309	NN	denotes	blood
T8798	34310-34315	NNS	denotes	cells
T8800	34316-34319	CC	denotes	and
T8801	34320-34329	NNS	denotes	platelets
T8792	34330-34333	VBP	denotes	are
T8802	34334-34337	RB	denotes	not
T8803	34338-34348	VBN	denotes	calculated
T8804	34349-34355	NNS	denotes	values
T8805	34355-34357	,	denotes	,
T8806	34357-34360	CC	denotes	but
T8807	34361-34369	RB	denotes	directly
T8808	34370-34378	VBN	denotes	measured
T8809	34379-34385	NNS	denotes	values
T8810	34385-34386	.	denotes	.
T8811	34386-34660	sentence	denotes	The following data were statistically analysed: leucocytes, red blood cells, haemoglobin, haematocrit, mean cellular volume, mean cellular haemoglobin, mean cellular haemoglobin concentration, platelet number, neutrophiles, lymphocytes, monocytes, eosinophiles, basophiles.
T8812	34387-34390	DT	denotes	The
T8814	34391-34400	VBG	denotes	following
T8813	34401-34405	NNS	denotes	data
T8816	34406-34410	VBD	denotes	were
T8817	34411-34424	RB	denotes	statistically
T8815	34425-34433	VBN	denotes	analysed
T8818	34433-34435	:	denotes	:
T8819	34435-34445	NNS	denotes	leucocytes
T8820	34445-34447	,	denotes	,
T8821	34447-34450	JJ	denotes	red
T8823	34451-34456	NN	denotes	blood
T8822	34457-34462	NNS	denotes	cells
T8824	34462-34464	,	denotes	,
T8825	34464-34475	NN	denotes	haemoglobin
T8826	34475-34477	,	denotes	,
T8827	34477-34488	NN	denotes	haematocrit
T8828	34488-34490	,	denotes	,
T8829	34490-34494	JJ	denotes	mean
T8831	34495-34503	JJ	denotes	cellular
T8830	34504-34510	NN	denotes	volume
T8832	34510-34512	,	denotes	,
T8833	34512-34516	JJ	denotes	mean
T8835	34517-34525	JJ	denotes	cellular
T8834	34526-34537	NN	denotes	haemoglobin
T8836	34537-34539	,	denotes	,
T8837	34539-34543	JJ	denotes	mean
T8839	34544-34552	JJ	denotes	cellular
T8838	34553-34564	NN	denotes	haemoglobin
T8840	34565-34578	NN	denotes	concentration
T8841	34578-34580	,	denotes	,
T8842	34580-34588	NN	denotes	platelet
T8843	34589-34595	NN	denotes	number
T8844	34595-34597	,	denotes	,
T8845	34597-34609	NNS	denotes	neutrophiles
T8846	34609-34611	,	denotes	,
T8847	34611-34622	NNS	denotes	lymphocytes
T8848	34622-34624	,	denotes	,
T8849	34624-34633	NNS	denotes	monocytes
T8850	34633-34635	,	denotes	,
T8851	34635-34647	NNS	denotes	eosinophiles
T8852	34647-34649	,	denotes	,
T8853	34649-34659	NNS	denotes	basophiles
T8854	34659-34660	.	denotes	.
T8855	34660-34753	sentence	denotes	Peptide mass fingerprinting was performed on a Bruker Reflex IV MALDI-TOF mass spectrometer.
T8856	34661-34668	NN	denotes	Peptide
T8857	34669-34673	NN	denotes	mass
T8858	34674-34688	NN	denotes	fingerprinting
T8860	34689-34692	VBD	denotes	was
T8859	34693-34702	VBN	denotes	performed
T8861	34703-34705	IN	denotes	on
T8862	34706-34707	DT	denotes	a
T8864	34708-34714	NNP	denotes	Bruker
T8865	34715-34721	NNP	denotes	Reflex
T8866	34722-34724	CD	denotes	IV
T8867	34725-34730	NN	denotes	MALDI
T8869	34730-34731	HYPH	denotes	-
T8868	34731-34734	NN	denotes	TOF
T8870	34735-34739	NN	denotes	mass
T8863	34740-34752	NN	denotes	spectrometer
T8871	34752-34753	.	denotes	.
T8872	34753-34878	sentence	denotes	Recombinant annexin A7 was purified according to [27], F-actin-binding of recombinant annexin A7 was done as described [28].
T8873	34754-34765	JJ	denotes	Recombinant
T8875	34766-34773	NN	denotes	annexin
T8874	34774-34776	NN	denotes	A7
T8877	34777-34780	VBD	denotes	was
T8876	34781-34789	VBN	denotes	purified
T8879	34790-34799	VBG	denotes	according
T8880	34800-34802	IN	denotes	to
T8881	34803-34804	-LRB-	denotes	[
T8882	34804-34806	CD	denotes	27
T8883	34806-34807	-RRB-	denotes	]
T8884	34807-34809	,	denotes	,
T8885	34809-34810	NN	denotes	F
T8887	34810-34811	HYPH	denotes	-
T8886	34811-34816	NN	denotes	actin
T8888	34816-34817	HYPH	denotes	-
T8889	34817-34824	VBG	denotes	binding
T8890	34825-34827	IN	denotes	of
T8891	34828-34839	JJ	denotes	recombinant
T8893	34840-34847	NN	denotes	annexin
T8892	34848-34850	NN	denotes	A7
T8894	34851-34854	VBD	denotes	was
T8878	34855-34859	VBN	denotes	done
T8895	34860-34862	IN	denotes	as
T8896	34863-34872	VBN	denotes	described
T8897	34873-34874	-LRB-	denotes	[
T8898	34874-34876	CD	denotes	28
T8899	34876-34877	-RRB-	denotes	]
T8900	34877-34878	.	denotes	.
T8901	34878-34940	sentence	denotes	SDS-PAGE and western blotting were done as described [29,30].
T8902	34879-34882	NN	denotes	SDS
T8904	34882-34883	HYPH	denotes	-
T8903	34883-34887	NN	denotes	PAGE
T8906	34888-34891	CC	denotes	and
T8907	34892-34899	JJ	denotes	western
T8908	34900-34908	NN	denotes	blotting
T8909	34909-34913	VBD	denotes	were
T8905	34914-34918	VBN	denotes	done
T8910	34919-34921	IN	denotes	as
T8911	34922-34931	VBN	denotes	described
T8912	34932-34933	-LRB-	denotes	[
T8914	34933-34935	CD	denotes	29
T8915	34935-34936	,	denotes	,
T8913	34936-34938	CD	denotes	30
T8916	34938-34939	-RRB-	denotes	]
T8917	34939-34940	.	denotes	.
T8918	34940-35007	sentence	denotes	Detection in immunoblots was with enhanced chemiluminescence [17].
T8919	34941-34950	NN	denotes	Detection
T8921	34951-34953	IN	denotes	in
T8922	34954-34965	NNS	denotes	immunoblots
T8920	34966-34969	VBD	denotes	was
T8923	34970-34974	IN	denotes	with
T8924	34975-34983	VBN	denotes	enhanced
T8925	34984-35001	NN	denotes	chemiluminescence
T8926	35002-35003	-LRB-	denotes	[
T8927	35003-35005	CD	denotes	17
T8928	35005-35006	-RRB-	denotes	]
T8929	35006-35007	.	denotes	.
T8930	35007-35145	sentence	denotes	Antibodies employed were mab 203–217 directed against mouse annexin A7 core domain [5] and a polyclonal rabbit anti-sorcin antibody [31].
T8931	35008-35018	NNS	denotes	Antibodies
T8933	35019-35027	VBN	denotes	employed
T8932	35028-35032	VBD	denotes	were
T8934	35033-35036	NN	denotes	mab
T8935	35037-35040	CD	denotes	203
T8936	35040-35041	SYM	denotes	–
T8937	35041-35044	CD	denotes	217
T8938	35045-35053	VBN	denotes	directed
T8939	35054-35061	IN	denotes	against
T8940	35062-35067	NN	denotes	mouse
T8942	35068-35075	NN	denotes	annexin
T8943	35076-35078	NN	denotes	A7
T8944	35079-35083	NN	denotes	core
T8941	35084-35090	NN	denotes	domain
T8945	35091-35092	-LRB-	denotes	[
T8946	35092-35093	CD	denotes	5
T8947	35093-35094	-RRB-	denotes	]
T8948	35095-35098	CC	denotes	and
T8949	35099-35100	DT	denotes	a
T8951	35101-35111	JJ	denotes	polyclonal
T8952	35112-35118	NN	denotes	rabbit
T8953	35119-35130	JJ	denotes	anti-sorcin
T8950	35131-35139	NN	denotes	antibody
T8954	35140-35141	-LRB-	denotes	[
T8955	35141-35143	CD	denotes	31
T8956	35143-35144	-RRB-	denotes	]
T8957	35144-35145	.	denotes	.
T8958	35145-35462	sentence	denotes	For statistical analyses the following tests were performed: David et al. range test to ensure samples are from a normal distribution, F-test looking for a samples' homoscedascity, Student's T-test to judge if samples of different groups have a different mean value and are parts from different normal distributions.
T8959	35146-35149	IN	denotes	For
T8961	35150-35161	JJ	denotes	statistical
T8962	35162-35170	NNS	denotes	analyses
T8963	35171-35174	DT	denotes	the
T8965	35175-35184	VBG	denotes	following
T8964	35185-35190	NNS	denotes	tests
T8966	35191-35195	VBD	denotes	were
T8960	35196-35205	VBN	denotes	performed
T8967	35205-35207	:	denotes	:
T8968	35207-35212	NNP	denotes	David
T8970	35213-35215	FW	denotes	et
T8971	35216-35219	FW	denotes	al.
T8972	35220-35225	NN	denotes	range
T8969	35226-35230	NN	denotes	test
T8973	35231-35233	TO	denotes	to
T8974	35234-35240	VB	denotes	ensure
T8975	35241-35248	NNS	denotes	samples
T8976	35249-35252	VBP	denotes	are
T8977	35253-35257	IN	denotes	from
T8978	35258-35259	DT	denotes	a
T8980	35260-35266	JJ	denotes	normal
T8979	35267-35279	NN	denotes	distribution
T8981	35279-35281	,	denotes	,
T8982	35281-35282	NN	denotes	F
T8984	35282-35283	HYPH	denotes	-
T8983	35283-35287	NN	denotes	test
T8985	35288-35295	VBG	denotes	looking
T8986	35296-35299	IN	denotes	for
T8987	35300-35301	DT	denotes	a
T8988	35302-35309	NNS	denotes	samples
T8990	35309-35310	POS	denotes	'
T8989	35311-35325	NN	denotes	homoscedascity
T8991	35325-35327	,	denotes	,
T8992	35327-35334	NN	denotes	Student
T8994	35334-35336	POS	denotes	's
T8995	35337-35338	NN	denotes	T
T8996	35338-35339	HYPH	denotes	-
T8993	35339-35343	NN	denotes	test
T8997	35344-35346	TO	denotes	to
T8998	35347-35352	VB	denotes	judge
T8999	35353-35355	IN	denotes	if
T9001	35356-35363	NNS	denotes	samples
T9002	35364-35366	IN	denotes	of
T9003	35367-35376	JJ	denotes	different
T9004	35377-35383	NNS	denotes	groups
T9000	35384-35388	VBP	denotes	have
T9005	35389-35390	DT	denotes	a
T9007	35391-35400	JJ	denotes	different
T9008	35401-35405	JJ	denotes	mean
T9006	35406-35411	NN	denotes	value
T9009	35412-35415	CC	denotes	and
T9010	35416-35419	VBP	denotes	are
T9011	35420-35425	NNS	denotes	parts
T9012	35426-35430	IN	denotes	from
T9013	35431-35440	JJ	denotes	different
T9015	35441-35447	JJ	denotes	normal
T9014	35448-35461	NNS	denotes	distributions
T9016	35461-35462	.	denotes	.
T9017	35462-35577	sentence	denotes	The exact probability values and the significance of an analysis are indicated when the experiments are described.
T9018	35463-35466	DT	denotes	The
T9020	35467-35472	JJ	denotes	exact
T9021	35473-35484	NN	denotes	probability
T9019	35485-35491	NNS	denotes	values
T9023	35492-35495	CC	denotes	and
T9024	35496-35499	DT	denotes	the
T9025	35500-35512	NN	denotes	significance
T9026	35513-35515	IN	denotes	of
T9027	35516-35518	DT	denotes	an
T9028	35519-35527	NN	denotes	analysis
T9029	35528-35531	VBP	denotes	are
T9022	35532-35541	VBN	denotes	indicated
T9030	35542-35546	WRB	denotes	when
T9032	35547-35550	DT	denotes	the
T9033	35551-35562	NNS	denotes	experiments
T9034	35563-35566	VBP	denotes	are
T9031	35567-35576	VBN	denotes	described
T9035	35576-35577	.	denotes	.
R1	T208	T205	punct	", ",Function
R10	T217	T216	cc	and,platelets
R100	T317	T318	det	The,role
R1000	T1862	T1852	acomp	healthy,is
R1001	T1863	T1862	punct	", ",healthy
R1002	T1942	T1941	compound	blood,cells
R1003	T1864	T1852	punct	", ",is
R1004	T1865	T1852	cc	and,is
R1005	T1943	T1944	punct	[,7
R1006	T1866	T1852	conj	shows,is
R1007	T1867	T1868	det	no,defect
R1008	T1868	T1866	dobj	defect,shows
R1009	T1944	T1930	parataxis	7,reported
R101	T318	T319	nsubjpass	role,addressed
R1010	T1869	T1870	compound	insulin,secretion
R1011	T1870	T1868	compound	secretion,defect
R1012	T1945	T1944	punct	],7
R1013	T1871	T1868	cc	or,defect
R1014	T1946	T1914	punct	.,thought
R1015	T1872	T1873	amod	other,defects
R1016	T1948	T1949	amod	Red,cells
R1017	T1873	T1868	conj	defects,defect
R1018	T1874	T1873	amod	obvious,defects
R1019	T1875	T1852	punct	.,is
R102	T320	T318	prep	of,role
R1020	T1949	T1951	nsubj	cells,undergo
R1021	T1877	T1878	advmod	However,disturbed
R1022	T1950	T1949	compound	blood,cells
R1023	T1879	T1878	punct	", ",disturbed
R1024	T1880	T1878	prep	in,disturbed
R1025	T1952	T1953	amod	various,changes
R1026	T1881	T1882	amod	isolated,cardiomyocytes
R1027	T1882	T1880	pobj	cardiomyocytes,in
R1028	T1883	T1884	det	the,shortening
R1029	T1953	T1951	dobj	changes,undergo
R103	T321	T322	compound	annexin,A7
R1030	T1884	T1878	nsubjpass	shortening,disturbed
R1031	T1885	T1886	npadvmod	frequency,induced
R1032	T1954	T1953	amod	biochemical,changes
R1033	T1886	T1884	amod	induced,shortening
R1034	T1887	T1886	punct	-,induced
R1035	T1888	T1878	auxpass	is,disturbed
R1036	T1889	T1878	punct	.,disturbed
R1037	T1955	T1954	cc	or,biochemical
R1038	T1891	T1892	advmod	Here,focused
R1039	T1956	T1954	conj	morphological,biochemical
R104	T322	T320	pobj	A7,of
R1040	T1893	T1892	nsubj	we,focused
R1041	T1957	T1958	dep	that,appear
R1042	T1894	T1892	aux	have,focused
R1043	T1895	T1892	prep	on,focused
R1044	T1896	T1897	det	the,analysis
R1045	T1958	T1953	relcl	appear,changes
R1046	T1897	T1895	pobj	analysis,on
R1047	T1898	T1897	prep	of,analysis
R1048	T1959	T1960	aux	to,be
R1049	T1899	T1900	amod	red,cells
R105	T323	T318	prep	in,role
R1050	T1900	T1898	pobj	cells,of
R1051	T1901	T1900	compound	blood,cells
R1052	T1960	T1958	xcomp	be,appear
R1053	T1902	T1900	cc	and,cells
R1054	T1903	T1900	conj	platelets,cells
R1055	T1904	T1900	prep	of,cells
R1056	T1961	T1962	npadvmod	Ca2+,dependent
R1057	T1905	T1906	det	the,mutant
R1058	T1906	T1904	pobj	mutant,of
R1059	T1907	T1906	nmod	anxA7,mutant
R106	T324	T325	amod	red,cells
R1060	T1962	T1964	amod	dependent,processes
R1061	T1908	T1907	punct	-,anxA7
R1062	T1909	T1907	punct	/,anxA7
R1063	T1963	T1962	punct	-,dependent
R1064	T1910	T1907	punct	-,anxA7
R1065	T1911	T1892	punct	.,focused
R1066	T1964	T1960	attr	processes,be
R1067	T1913	T1914	advmod	Generally,thought
R1068	T1915	T1914	punct	", ",thought
R1069	T1965	T1966	punct	[,19
R107	T325	T323	pobj	cells,in
R1070	T1916	T1917	amod	red,cells
R1071	T1917	T1914	nsubjpass	cells,thought
R1072	T1966	T1951	parataxis	19,undergo
R1073	T1918	T1917	compound	blood,cells
R1074	T1967	T1966	nummod	18,19
R1075	T1968	T1966	punct	",",19
R1076	T1969	T1966	punct	],19
R1077	T1970	T1951	punct	.,undergo
R1078	T1972	T1973	nsubj	One,is
R1079	T2026	T2027	dep	which,lack
R108	T326	T325	compound	blood,cells
R1080	T2027	T2023	relcl	lack,cells
R1081	T1974	T1972	prep	of,One
R1082	T2028	T2029	det	the,ability
R1083	T2029	T2027	dobj	ability,lack
R1084	T2030	T2031	aux	to,vesiculate
R1085	T1975	T1974	pobj	them,of
R1086	T2031	T2029	acl	vesiculate,ability
R1087	T2032	T2033	det	a,disease
R1088	T2033	T2025	dobj	disease,cause
R1089	T2034	T2025	prep	with,cause
R109	T327	T319	auxpass	was,addressed
R1090	T2035	T2036	amod	red,cell
R1091	T2036	T2038	compound	cell,destruction
R1092	T1976	T1977	det	the,release
R1093	T2037	T2036	compound	blood,cell
R1094	T2038	T2034	pobj	destruction,with
R1095	T2039	T2038	cc	and,destruction
R1096	T1977	T1973	attr	release,is
R1097	T2040	T2038	conj	haemoglobinuria,destruction
R1098	T2041	T2042	punct	[,21
R1099	T1978	T1977	prep	of,release
R11	T218	T219	amod	red,cells
R110	T328	T319	prep	in,addressed
R1100	T2042	T2025	parataxis	21,cause
R1101	T2043	T2042	punct	],21
R1102	T1979	T1980	npadvmod	hemoglobin,containing
R1103	T2044	T2025	punct	.,cause
R1104	T2046	T2047	expl	There,exist
R1105	T1980	T1982	amod	containing,exovesicles
R1106	T2048	T2049	nummod	two,types
R1107	T2049	T2047	dobj	types,exist
R1108	T1981	T1980	punct	-,containing
R1109	T1982	T1978	pobj	exovesicles,of
R111	T329	T328	pobj	erythrocytes,in
R1110	T2050	T2049	prep	of,types
R1111	T2051	T2050	pobj	vesicles,of
R1112	T1983	T1977	acl	occurring,release
R1113	T2052	T2049	punct	", ",types
R1114	T2053	T2054	nmod	micro,nanovesicles
R1115	T1984	T1985	advmod	in,vivo
R1116	T2054	T2049	appos	nanovesicles,types
R1117	T2055	T2054	punct	-,nanovesicles
R1118	T2056	T2054	cc	and,nanovesicles
R1119	T1985	T1983	advmod	vivo,occurring
R112	T330	T329	prep	from,erythrocytes
R1120	T2057	T2049	prep	with,types
R1121	T2058	T2059	det	a,size
R1122	T1986	T1987	advmod	as,as
R1123	T2059	T2057	pobj	size,with
R1124	T2060	T2059	prep	of,size
R1125	T1987	T1985	cc	as,vivo
R1126	T2061	T2062	nummod	180,nm
R1127	T2062	T2060	pobj	nm,of
R1128	T2063	T2062	cc	and,nm
R1129	T1988	T1987	advmod	well,as
R113	T331	T332	nmod	anxA7,mice
R1130	T2064	T2065	nummod	60,nm
R1131	T2065	T2062	conj	nm,nm
R1132	T1989	T1990	advmod	in,vitro
R1133	T2066	T2047	punct	", ",exist
R1134	T2067	T2047	advmod	respectively,exist
R1135	T2068	T2047	punct	.,exist
R1136	T1990	T1985	conj	vitro,vivo
R1137	T2070	T2071	nsubjpass	They,enriched
R1138	T1991	T1992	punct	[,20
R1139	T2072	T2071	auxpass	are,enriched
R114	T332	T330	pobj	mice,from
R1140	T2073	T2071	prep	in,enriched
R1141	T2074	T2075	npadvmod	cholesterol,rich
R1142	T2075	T2078	amod	rich,domains
R1143	T2076	T2074	cc	and,cholesterol
R1144	T1992	T1973	parataxis	20,is
R1145	T2077	T2074	conj	sphingolipid,cholesterol
R1146	T2078	T2073	pobj	domains,in
R1147	T2079	T2080	compound	lipid,raft
R1148	T1993	T1992	nummod	7,20
R1149	T2080	T2078	compound	raft,domains
R115	T333	T331	punct	-,anxA7
R1150	T2081	T2082	dep	that,associated
R1151	T2082	T2078	relcl	associated,domains
R1152	T1994	T1992	punct	",",20
R1153	T2083	T2082	auxpass	are,associated
R1154	T2084	T2082	prep	with,associated
R1155	T1995	T1992	punct	],20
R1156	T2085	T2084	pobj	proteins,with
R1157	T2086	T2085	prep	like,proteins
R1158	T1996	T1973	punct	.,is
R1159	T2087	T2086	pobj	acetylcholinesterase,like
R116	T334	T331	punct	/,anxA7
R1160	T2088	T2087	punct	", ",acetylcholinesterase
R1161	T2089	T2090	compound	cell,proteins
R1162	T1998	T1999	det	This,process
R1163	T2090	T2087	conj	proteins,acetylcholinesterase
R1164	T2091	T2090	compound	surface,proteins
R1165	T2092	T2090	prep	including,proteins
R1166	T1999	T2000	nsubjpass	process,thought
R1167	T2093	T2094	det	a,receptor
R1168	T2094	T2092	pobj	receptor,including
R1169	T2095	T2094	compound	complement,receptor
R117	T335	T331	punct	-,anxA7
R1170	T2001	T2000	auxpass	is,thought
R1171	T2096	T2090	punct	", ",proteins
R1172	T2097	T2090	cc	and,proteins
R1173	T2098	T2099	det	the,proteins
R1174	T2099	T2090	conj	proteins,proteins
R1175	T2002	T2003	aux	to,represent
R1176	T2100	T2101	compound	lipid,raft
R1177	T2101	T2099	compound	raft,proteins
R1178	T2102	T2099	appos	stomatin,proteins
R1179	T2003	T2000	xcomp	represent,thought
R118	T336	T319	punct	.,addressed
R1180	T2103	T2102	cc	and,stomatin
R1181	T2104	T2102	conj	flotillin,stomatin
R1182	T2105	T2071	punct	", ",enriched
R1183	T2004	T2005	det	a,method
R1184	T2106	T2071	cc	but,enriched
R1185	T2107	T2108	nsubj	they,lack
R1186	T2005	T2003	dobj	method,represent
R1187	T2108	T2071	conj	lack,enriched
R1188	T2109	T2110	det	any,protein
R1189	T2006	T2005	amod	protective,method
R119	T338	T339	advmod	Interestingly,impaired
R1190	T2110	T2108	dobj	protein,lack
R1191	T2111	T2110	amod	cytoskeletal,protein
R1192	T2112	T2113	punct	[,7
R1193	T2113	T2108	parataxis	7,lack
R1194	T2114	T2113	punct	],7
R1195	T2115	T2108	punct	.,lack
R1196	T2007	T2005	prep	of,method
R1197	T2117	T2118	advmod	Furthermore,contain
R1198	T2008	T2009	det	the,cell
R1199	T2119	T2118	punct	", ",contain
R12	T219	T216	conj	cells,platelets
R120	T340	T339	punct	", ",impaired
R1200	T2120	T2118	nsubj	they,contain
R1201	T2009	T2007	pobj	cell,of
R1202	T2121	T2118	dobj	sorcin,contain
R1203	T2122	T2121	cc	and,sorcin
R1204	T2010	T2009	amod	red,cell
R1205	T2123	T2124	compound	annexin,A7
R1206	T2124	T2121	conj	A7,sorcin
R1207	T2125	T2121	acl	attached,sorcin
R1208	T2011	T2009	compound	blood,cell
R1209	T2012	T2005	prep	against,method
R121	T341	T342	det	the,process
R1210	T2126	T2125	prep	to,attached
R1211	T2013	T2014	det	an,attack
R1212	T2127	T2128	det	the,rafts
R1213	T2128	T2126	pobj	rafts,to
R1214	T2014	T2012	pobj	attack,against
R1215	T2129	T2128	compound	lipid,rafts
R1216	T2130	T2118	punct	.,contain
R1217	T2015	T2014	prep	by,attack
R1218	T2016	T2017	prep	for,components
R1219	T2017	T2015	pobj	components,by
R122	T342	T339	nsubjpass	process,impaired
R1220	T2132	T2133	det	Both,proteins
R1221	T2133	T2134	nsubj	proteins,are
R1222	T2018	T2016	pobj	example,for
R1223	T2135	T2136	advmod	more,abundant
R1224	T2136	T2134	acomp	abundant,are
R1225	T2019	T2017	compound	complement,components
R1226	T2020	T2000	punct	.,thought
R1227	T2137	T2134	prep	in,are
R1228	T2138	T2137	pobj	nanovesicles,in
R1229	T2022	T2023	amod	Red,cells
R123	T343	T344	npadvmod	Ca2+,mediated
R1230	T2139	T2134	punct	.,are
R1231	T2141	T2142	det	The,formation
R1232	T2142	T2144	nsubj	formation,goes
R1233	T2023	T2025	nsubj	cells,cause
R1234	T2143	T2142	compound	vesicle,formation
R1235	T2024	T2023	compound	blood,cells
R1236	T2145	T2144	prt	along,goes
R1237	T2146	T2144	prep	with,goes
R1238	T2147	T2148	amod	several,changes
R1239	T2148	T2146	pobj	changes,with
R124	T344	T342	amod	mediated,process
R1240	T2149	T2148	amod	other,changes
R1241	T2150	T2148	prep	in,changes
R1242	T2151	T2152	det	the,cell
R1243	T2152	T2150	pobj	cell,in
R1244	T2153	T2152	amod	red,cell
R1245	T2154	T2152	compound	blood,cell
R1246	T2155	T2148	prep	like,changes
R1247	T2156	T2157	amod	cytoskeletal,rearrangements
R1248	T2157	T2155	pobj	rearrangements,like
R1249	T2158	T2157	cc	and,rearrangements
R125	T345	T344	punct	-,mediated
R1250	T2159	T2157	conj	changes,rearrangements
R1251	T2160	T2159	prep	in,changes
R1252	T2161	T2162	det	the,orientation
R1253	T2162	T2160	pobj	orientation,in
R1254	T2163	T2162	compound	phospholipid,orientation
R1255	T2164	T2159	prep	in,changes
R1256	T2165	T2166	det	the,membrane
R1257	T2166	T2164	pobj	membrane,in
R1258	T2167	T2166	amod	cellular,membrane
R1259	T2168	T2144	punct	.,goes
R126	T346	T342	compound	vesiculation,process
R1264	T2443	T2444	compound	Annexin,A7
R1265	T2444	T2445	compound	A7,expression
R1266	T2446	T2445	prep	in,expression
R1267	T2447	T2448	amod	red,cells
R1268	T2448	T2446	pobj	cells,in
R1269	T2449	T2448	compound	blood,cells
R127	T347	T339	auxpass	was,impaired
R1270	T2450	T2448	punct	", ",cells
R1271	T2451	T2452	amod	red,cell
R1272	T2452	T2454	npadvmod	cell,derived
R1273	T2453	T2452	compound	blood,cell
R1274	T2454	T2455	amod	derived,exovesicles
R1275	T2455	T2448	appos	exovesicles,cells
R1276	T2456	T2446	cc	and,in
R1277	T2457	T2446	conj	in,in
R1278	T2458	T2457	pobj	platelets,in
R1279	T2460	T2461	nsubj	Salzer,reported
R128	T348	T339	neg	not,impaired
R1280	T2462	T2463	advmod	et,al.
R1281	T2463	T2460	advmod	al.,Salzer
R1282	T2464	T2461	advmod	recently,reported
R1283	T2465	T2466	det	the,presence
R1284	T2466	T2461	dobj	presence,reported
R1285	T2467	T2466	prep	of,presence
R1286	T2468	T2469	det	the,isoform
R1287	T2469	T2467	pobj	isoform,of
R1288	T2470	T2471	nummod	47,kDa
R1289	T2471	T2469	compound	kDa,isoform
R129	T349	T339	punct	.,impaired
R1290	T2472	T2469	prep	of,isoform
R1291	T2473	T2474	compound	annexin,A7
R1292	T2474	T2472	pobj	A7,of
R1293	T2475	T2474	cc	and,A7
R1294	T2476	T2477	poss	its,partner
R1295	T2477	T2474	conj	partner,A7
R1296	T2478	T2477	appos	sorcin,partner
R1297	T2479	T2469	prep	in,isoform
R1298	T2480	T2479	pobj	micro,in
R1299	T2481	T2480	punct	-,micro
R13	T220	T219	compound	blood,cells
R130	T351	T352	advmod	Also,appeared
R1300	T2482	T2480	cc	and,micro
R1301	T2483	T2480	conj	nanovesicles,micro
R1302	T2484	T2480	acl	derived,micro
R1303	T2485	T2484	prep	from,derived
R1304	T2486	T2487	amod	red,cells
R1305	T2487	T2485	pobj	cells,from
R1306	T2488	T2487	compound	blood,cells
R1307	T2489	T2490	advmod	where,located
R1308	T2490	T2487	relcl	located,cells
R1309	T2491	T2490	nsubjpass	they,located
R131	T353	T352	punct	", ",appeared
R1310	T2492	T2490	auxpass	are,located
R1311	T2493	T2490	prep	in,located
R1312	T2494	T2495	det	the,lumen
R1313	T2495	T2493	pobj	lumen,in
R1314	T2496	T2490	cc	and,located
R1315	T2497	T2498	auxpass	are,enriched
R1316	T2498	T2490	conj	enriched,located
R1317	T2499	T2498	advmod	also,enriched
R1318	T2500	T2498	prep	in,enriched
R1319	T2501	T2502	compound	membrane,rafts
R132	T354	T355	det	the,organization
R1320	T2502	T2500	pobj	rafts,in
R1321	T2503	T2504	punct	[,7
R1322	T2504	T2461	parataxis	7,reported
R1323	T2505	T2504	punct	],7
R1324	T2506	T2461	punct	.,reported
R1325	T2508	T2509	nsubj	We,extended
R1326	T2510	T2509	aux	have,extended
R1327	T2511	T2509	advmod	now,extended
R1328	T2512	T2513	det	these,findings
R1329	T2513	T2509	dobj	findings,extended
R133	T355	T352	nsubj	organization,appeared
R1330	T2514	T2509	cc	and,extended
R1331	T2515	T2509	conj	show,extended
R1332	T2516	T2515	advmod	here,show
R1333	T2517	T2518	mark	that,is
R1334	T2518	T2515	ccomp	is,show
R1335	T2519	T2520	det	the,isoform
R1336	T2520	T2518	nsubj	isoform,is
R1337	T2521	T2522	nummod	51,kDa
R1338	T2522	T2520	compound	kDa,isoform
R1339	T2523	T2518	acomp	present,is
R134	T356	T355	compound	membrane,organization
R1340	T2524	T2525	advmod	as,well
R1341	T2525	T2518	advmod	well,is
R1342	T2526	T2509	punct	.,extended
R1343	T2528	T2529	prep	In,detected
R1344	T2530	T2531	amod	western,blots
R1345	T2531	T2528	pobj	blots,In
R1346	T2532	T2531	prep	of,blots
R1347	T2533	T2534	amod	human,cells
R1348	T2534	T2532	pobj	cells,of
R1349	T2535	T2534	amod	red,cells
R135	T357	T358	neg	not,disturbed
R1350	T2536	T2534	compound	blood,cells
R1351	T2537	T2538	det	the,isoforms
R1352	T2538	T2529	nsubjpass	isoforms,detected
R1353	T2539	T2540	nummod	47,kDa
R1354	T2540	T2538	compound	kDa,isoforms
R1355	T2541	T2539	cc	and,47
R1356	T2542	T2539	conj	51,47
R1357	T2543	T2529	auxpass	were,detected
R1358	T2544	T2529	advcl	using,detected
R1359	T2545	T2544	dobj	mAb,using
R136	T358	T352	xcomp	disturbed,appeared
R1360	T2546	T2545	nummod	203–217,mAb
R1361	T2547	T2548	punct	(,Fig.
R1362	T2548	T2529	parataxis	Fig.,detected
R1363	T2549	T2548	nummod	1,Fig.
R1364	T2550	T2548	punct	),Fig.
R1365	T2551	T2529	punct	.,detected
R1366	T2553	T2554	det	The,isoform
R1367	T2554	T2557	nsubjpass	isoform,detected
R1368	T2555	T2556	nummod	47,kDa
R1369	T2556	T2554	compound	kDa,isoform
R137	T359	T358	aux	to,disturbed
R1370	T2558	T2557	auxpass	was,detected
R1371	T2559	T2557	prep	in,detected
R1372	T2560	T2561	npadvmod	silver,stained
R1373	T2561	T2562	amod	stained,gels
R1374	T2562	T2559	pobj	gels,in
R1375	T2563	T2557	cc	and,detected
R1376	T2564	T2565	poss	its,identity
R1377	T2565	T2566	nsubj	identity,confirmed
R1378	T2566	T2557	conj	confirmed,detected
R1379	T2567	T2565	prep	with,identity
R138	T360	T358	auxpass	be,disturbed
R1380	T2568	T2569	compound	annexin,A7
R1381	T2569	T2567	pobj	A7,with
R1382	T2570	T2566	prep	by,confirmed
R1383	T2571	T2572	compound	peptide,fingerprinting
R1384	T2572	T2570	pobj	fingerprinting,by
R1385	T2573	T2572	compound	mass,fingerprinting
R1386	T2574	T2575	punct	(,shown
R1387	T2575	T2566	parataxis	shown,confirmed
R1388	T2576	T2575	nsubj	data,shown
R1389	T2577	T2575	neg	not,shown
R139	T361	T362	mark	as,assessed
R1390	T2578	T2575	punct	),shown
R1391	T2579	T2566	punct	.,confirmed
R1392	T2581	T2582	det	The,isoform
R1393	T2582	T2585	nsubjpass	isoform,detected
R1394	T2583	T2584	nummod	51,kDa
R1395	T2584	T2582	compound	kDa,isoform
R1396	T2586	T2585	auxpass	was,detected
R1397	T2587	T2585	advmod	only,detected
R1398	T2588	T2585	prep	in,detected
R1399	T2589	T2590	amod	western,blots
R14	T221	T206	punct	: ,Insights
R140	T362	T358	advcl	assessed,disturbed
R1400	T2590	T2588	pobj	blots,in
R1401	T2591	T2585	punct	.,detected
R1402	T2594	T2595	poss	Its,presence
R1403	T2595	T2596	nsubj	presence,led
R1404	T2597	T2595	prep	in,presence
R1405	T2598	T2597	pobj	vesicles,in
R1406	T2599	T2596	prep	to,led
R1407	T2600	T2601	det	the,suggestion
R1408	T2601	T2599	pobj	suggestion,to
R1409	T2602	T2601	punct	", ",suggestion
R141	T363	T362	advcl	using,assessed
R1410	T2603	T2604	mark	that,plays
R1411	T2604	T2601	acl	plays,suggestion
R1412	T2605	T2604	punct	", ",plays
R1413	T2606	T2604	prep	along,plays
R1414	T2607	T2606	prep	with,along
R1415	T2608	T2609	compound	raft,domains
R1416	T2609	T2607	pobj	domains,with
R1417	T2610	T2604	punct	", ",plays
R1418	T2611	T2612	compound	annexin,A7
R1419	T2612	T2604	nsubj	A7,plays
R142	T364	T365	compound	gradient,fractionation
R1420	T2613	T2614	det	a,role
R1421	T2614	T2604	dobj	role,plays
R1422	T2615	T2604	prep	in,plays
R1423	T2616	T2617	compound	membrane,organization
R1424	T2617	T2615	pobj	organization,in
R1425	T2618	T2617	cc	and,organization
R1426	T2619	T2620	det	the,process
R1427	T2620	T2617	conj	process,organization
R1428	T2621	T2620	compound	vesiculation,process
R1429	T2622	T2596	punct	.,led
R143	T365	T366	compound	fractionation,studies
R1430	T2624	T2625	aux	To,examine
R1431	T2625	T2626	advcl	examine,analysed
R1432	T2627	T2625	dobj	this,examine
R1433	T2628	T2626	punct	", ",analysed
R1434	T2629	T2626	nsubj	we,analysed
R1435	T2630	T2631	det	the,ability
R1436	T2631	T2626	dobj	ability,analysed
R1437	T2632	T2631	prep	of,ability
R1438	T2633	T2634	amod	red,cells
R1439	T2634	T2632	pobj	cells,of
R144	T366	T363	dobj	studies,using
R1440	T2635	T2634	compound	blood,cells
R1441	T2636	T2634	acl	derived,cells
R1442	T2637	T2636	prep	from,derived
R1443	T2638	T2639	det	the,mice
R1444	T2639	T2637	pobj	mice,from
R1445	T2640	T2641	compound	annexin,A7
R1446	T2641	T2639	compound	A7,mice
R1447	T2642	T2643	compound	knock,out
R1448	T2643	T2639	compound	out,mice
R1449	T2644	T2645	punct	(,anxA7
R145	T367	T352	punct	.,appeared
R1450	T2645	T2639	parataxis	anxA7,mice
R1451	T2646	T2645	punct	-,anxA7
R1452	T2647	T2645	punct	/,anxA7
R1453	T2648	T2645	punct	-,anxA7
R1454	T2649	T2645	punct	),anxA7
R1455	T2650	T2651	aux	to,form
R1456	T2651	T2631	acl	form,ability
R1457	T2652	T2651	dobj	exovesicles,form
R1458	T2653	T2626	punct	.,analysed
R1459	T2655	T2656	prep	Because,included
R146	T369	T370	advmod	Instead,led
R1460	T2657	T2655	pcomp	of,Because
R1461	T2658	T2659	amod	better,accessibility
R1462	T2659	T2655	pobj	accessibility,Because
R1463	T2660	T2659	cc	and,accessibility
R1464	T2661	T2662	amod	larger,amounts
R1465	T2662	T2659	conj	amounts,accessibility
R1466	T2663	T2662	amod	available,amounts
R1467	T2664	T2665	dep	that,led
R1468	T2665	T2659	relcl	led,accessibility
R1469	T2666	T2665	prep	to,led
R147	T371	T370	punct	", ",led
R1470	T2667	T2668	amod	clear,results
R1471	T2668	T2666	pobj	results,to
R1472	T2669	T2656	punct	", ",included
R1473	T2670	T2656	nsubj	we,included
R1474	T2671	T2672	poss	our,data
R1475	T2672	T2656	dobj	data,included
R1476	T2673	T2672	acl	obtained,data
R1477	T2674	T2673	prep	with,obtained
R1478	T2675	T2676	amod	human,blood
R1479	T2676	T2674	pobj	blood,with
R148	T372	T370	nsubj	lack,led
R1480	T2677	T2656	punct	.,included
R1481	T2679	T2680	advcl	Independent,released
R1482	T2681	T2679	prep	of,Independent
R1483	T2682	T2683	det	the,presence
R1484	T2683	T2681	pobj	presence,of
R1485	T2684	T2683	prep	of,presence
R1486	T2685	T2686	compound	annexin,A7
R1487	T2686	T2684	pobj	A7,of
R1488	T2687	T2688	det	both,types
R1489	T2688	T2680	nsubjpass	types,released
R149	T373	T372	prep	of,lack
R1490	T2689	T2688	prep	of,types
R1491	T2690	T2689	pobj	exovesicles,of
R1492	T2691	T2688	punct	", ",types
R1493	T2692	T2693	nmod	micro,nanovesicles
R1494	T2693	T2688	appos	nanovesicles,types
R1495	T2694	T2693	punct	-,nanovesicles
R1496	T2695	T2693	cc	and,nanovesicles
R1497	T2696	T2680	punct	", ",released
R1498	T2697	T2680	auxpass	were,released
R1499	T2698	T2680	prep	after,released
R15	T222	T206	acl	derived,Insights
R150	T374	T375	compound	annexin,A7
R1500	T2699	T2700	compound	Ca2+,ionophore
R1501	T2700	T2702	compound	ionophore,treatment
R1502	T2701	T2700	punct	/,ionophore
R1503	T2702	T2698	pobj	treatment,after
R1504	T2703	T2704	mark	as,determined
R1505	T2704	T2680	advcl	determined,released
R1506	T2705	T2704	agent	by,determined
R1507	T2706	T2707	compound	acetylcholine,esterase
R1508	T2707	T2708	compound	esterase,activity
R1509	T2708	T2705	pobj	activity,by
R151	T375	T373	pobj	A7,of
R1510	T2709	T2708	cc	and,activity
R1511	T2710	T2711	amod	microscopic,examination
R1512	T2711	T2708	conj	examination,activity
R1513	T2712	T2680	punct	.,released
R1514	T2714	T2715	compound	Annexin,A7
R1515	T2715	T2716	nsubj	A7,is
R1516	T2717	T2716	acomp	present,is
R1517	T2718	T2716	prep	in,is
R1518	T2719	T2720	preconj	both,types
R1519	T2720	T2718	pobj	types,in
R152	T376	T370	prep	to,led
R1520	T2721	T2720	compound	vesicle,types
R1521	T2722	T2723	advmod	where,is
R1522	T2723	T2720	advcl	is,types
R1523	T2724	T2723	nsubj	it,is
R1524	T2725	T2726	advmod	more,enriched
R1525	T2726	T2723	acomp	enriched,is
R1526	T2727	T2723	prep	in,is
R1527	T2728	T2727	pobj	nanovesicles,in
R1528	T2729	T2730	punct	(,Fig.
R1529	T2730	T2716	parataxis	Fig.,is
R153	T377	T378	det	an,shape
R1530	T2731	T2730	nummod	2,Fig.
R1531	T2732	T2730	punct	),Fig.
R1532	T2733	T2716	punct	.,is
R1533	T2735	T2736	det	The,isoform
R1534	T2736	T2739	nsubj	isoform,is
R1535	T2737	T2738	nummod	51,kDa
R1536	T2738	T2736	compound	kDa,isoform
R1537	T2740	T2739	advmod	only,is
R1538	T2741	T2739	acomp	detectable,is
R1539	T2742	T2739	prep	in,is
R154	T378	T376	pobj	shape,to
R1540	T2743	T2742	pobj	nanovesicles,in
R1541	T2744	T2739	punct	.,is
R1542	T2746	T2747	det	The,quantity
R1543	T2747	T2748	nsubj	quantity,differ
R1544	T2749	T2747	prep	of,quantity
R1545	T2750	T2751	det	both,types
R1546	T2751	T2749	pobj	types,of
R1547	T2752	T2751	compound	vesicle,types
R1548	T2753	T2748	aux	did,differ
R1549	T2754	T2748	neg	not,differ
R155	T379	T378	amod	altered,shape
R1550	T2755	T2748	prep	between,differ
R1551	T2756	T2757	amod	wild,type
R1552	T2757	T2758	nmod	type,vesicles
R1553	T2758	T2755	pobj	vesicles,between
R1554	T2759	T2757	cc	and,type
R1555	T2760	T2761	compound	knock,out
R1556	T2761	T2757	conj	out,type
R1557	T2762	T2763	amod	red,cell
R1558	T2763	T2758	compound	cell,vesicles
R1559	T2764	T2763	compound	blood,cell
R156	T380	T378	compound	cell,shape
R1560	T2765	T2766	mark	as,determined
R1561	T2766	T2748	advcl	determined,differ
R1562	T2767	T2766	agent	by,determined
R1563	T2768	T2769	det	the,values
R1564	T2769	T2767	pobj	values,by
R1565	T2770	T2769	compound	AChE,values
R1566	T2771	T2769	punct	-,values
R1567	T2772	T2773	punct	(,both
R1568	T2773	T2769	parataxis	both,values
R1569	T2774	T2775	compound	A405,nanovesicles
R157	T381	T378	cc	and,shape
R1570	T2775	T2776	dep	nanovesicles,0.18
R1571	T2776	T2773	dep	0.18,both
R1572	T2777	T2776	punct	: ,0.18
R1573	T2778	T2776	punct	~,0.18
R1574	T2779	T2773	punct	;,both
R1575	T2780	T2781	compound	A405,microvesicles
R1576	T2781	T2782	dep	microvesicles,0.9
R1577	T2782	T2773	dep	0.9,both
R1578	T2783	T2782	punct	: ,0.9
R1579	T2784	T2782	punct	~,0.9
R158	T382	T383	amod	increased,resistance
R1580	T2785	T2773	punct	;,both
R1581	T2786	T2773	punct	", ",both
R1582	T2787	T2773	prep	for,both
R1583	T2788	T2787	pobj	wt,for
R1584	T2789	T2788	cc	and,wt
R1585	T2790	T2788	conj	ko,wt
R1586	T2791	T2773	punct	),both
R1587	T2792	T2748	punct	.,differ
R1588	T2794	T2795	nsubj	It,appears
R1589	T2796	T2797	mark	that,is
R159	T383	T378	conj	resistance,shape
R1590	T2797	T2795	ccomp	is,appears
R1591	T2798	T2799	compound	annexin,A7
R1592	T2799	T2797	nsubj	A7,is
R1593	T2800	T2797	punct	", ",is
R1594	T2801	T2802	mark	although,described
R1595	T2802	T2797	advcl	described,is
R1596	T2803	T2802	nsubjpass	it,described
R1597	T2804	T2802	aux	has,described
R1598	T2805	T2802	auxpass	been,described
R1599	T2806	T2807	aux	to,fuse
R16	T223	T222	prep	from,derived
R160	T384	T383	amod	osmotic,resistance
R1600	T2807	T2802	xcomp	fuse,described
R1601	T2808	T2807	dobj	membranes,fuse
R1602	T2809	T2797	punct	", ",is
R1603	T2810	T2797	neg	not,is
R1604	T2811	T2812	det	a,component
R1605	T2812	T2797	attr	component,is
R1606	T2813	T2812	amod	key,component
R1607	T2814	T2812	prep	in,component
R1608	T2815	T2816	det	the,process
R1609	T2816	T2814	pobj	process,in
R161	T385	T378	prep	of,shape
R1610	T2817	T2816	prep	of,process
R1611	T2818	T2819	det	the,formation
R1612	T2819	T2817	pobj	formation,of
R1613	T2820	T2819	prep	of,formation
R1614	T2821	T2822	amod	red,cell
R1615	T2822	T2824	compound	cell,exovesicles
R1616	T2823	T2822	compound	blood,cell
R1617	T2824	T2820	pobj	exovesicles,of
R1618	T2825	T2795	punct	.,appears
R1619	T2827	T2828	advmod	When,probed
R162	T386	T387	amod	red,cells
R1620	T2828	T2830	advcl	probed,found
R1621	T2829	T2828	nsubj	we,probed
R1622	T2831	T2828	prep	for,probed
R1623	T2832	T2833	det	the,presence
R1624	T2833	T2831	pobj	presence,for
R1625	T2834	T2833	prep	of,presence
R1626	T2835	T2834	pobj	sorcin,of
R1627	T2836	T2828	prep	in,probed
R1628	T2837	T2838	amod	wild,type
R1629	T2838	T2839	nmod	type,vesicles
R163	T387	T385	pobj	cells,of
R1630	T2839	T2836	pobj	vesicles,in
R1631	T2840	T2838	cc	and,type
R1632	T2841	T2838	conj	mutant,type
R1633	T2842	T2830	nsubj	we,found
R1634	T2843	T2844	mark	that,reduced
R1635	T2844	T2830	ccomp	reduced,found
R1636	T2845	T2846	det	the,levels
R1637	T2846	T2844	nsubjpass	levels,reduced
R1638	T2847	T2846	compound	sorcin,levels
R1639	T2848	T2844	auxpass	were,reduced
R164	T388	T387	compound	blood,cells
R1640	T2849	T2844	prep	in,reduced
R1641	T2850	T2851	det	the,nanovesicles
R1642	T2851	T2849	pobj	nanovesicles,in
R1643	T2852	T2851	amod	mutant,nanovesicles
R1644	T2853	T2854	punct	(,Fig.
R1645	T2854	T2830	parataxis	Fig.,found
R1646	T2855	T2854	nummod	2,Fig.
R1647	T2856	T2854	punct	),Fig.
R1648	T2857	T2830	punct	.,found
R1649	T2859	T2860	aux	To,study
R165	T389	T370	punct	.,led
R1650	T2860	T2861	advcl	study,used
R1651	T2862	T2863	det	the,distribution
R1652	T2863	T2860	dobj	distribution,study
R1653	T2864	T2863	prep	of,distribution
R1654	T2865	T2866	compound	annexin,A7
R1655	T2866	T2864	pobj	A7,of
R1656	T2867	T2860	prep	in,study
R1657	T2868	T2869	amod	red,cells
R1658	T2869	T2867	pobj	cells,in
R1659	T2870	T2869	compound	blood,cells
R166	T391	T392	compound	Annexin,A7
R1660	T2871	T2861	nsubj	we,used
R1661	T2872	T2873	npadvmod	self,forming
R1662	T2873	T2874	amod	forming,gradients
R1663	T2874	T2861	dobj	gradients,used
R1664	T2875	T2874	compound	iodixanol,gradients
R1665	T2876	T2874	compound	density,gradients
R1666	T2877	T2861	punct	.,used
R1667	T2879	T2880	det	Both,isoforms
R1668	T2880	T2881	nsubj	isoforms,were
R1669	T2882	T2881	acomp	present,were
R167	T392	T393	nsubjpass	A7,identified
R1670	T2883	T2881	prep	in,were
R1671	T2884	T2885	det	the,fraction
R1672	T2885	T2883	pobj	fraction,in
R1673	T2886	T2885	amod	soluble,fraction
R1674	T2887	T2888	advmod	as,as
R1675	T2888	T2883	cc	as,in
R1676	T2889	T2888	advmod	well,as
R1677	T2890	T2883	conj	in,in
R1678	T2891	T2892	det	the,fractions
R1679	T2892	T2890	pobj	fractions,in
R168	T394	T393	auxpass	was,identified
R1680	T2893	T2892	compound	gradient,fractions
R1681	T2894	T2895	advmod	where,assumed
R1682	T2895	T2892	relcl	assumed,fractions
R1683	T2896	T2895	nsubjpass	they,assumed
R1684	T2897	T2895	auxpass	are,assumed
R1685	T2898	T2899	aux	to,associated
R1686	T2899	T2895	xcomp	associated,assumed
R1687	T2900	T2899	auxpass	be,associated
R1688	T2901	T2899	prep	with,associated
R1689	T2902	T2901	pobj	membranes,with
R169	T395	T393	advmod	also,identified
R1690	T2903	T2881	punct	.,were
R1691	T2905	T2906	det	These,membranes
R1692	T2906	T2907	nsubj	membranes,are
R1693	T2908	T2907	advmod	exclusively,are
R1694	T2909	T2910	compound	plasma,membranes
R1695	T2910	T2907	attr	membranes,are
R1696	T2911	T2912	mark	as,are
R1697	T2912	T2907	advcl	are,are
R1698	T2913	T2914	amod	red,cells
R1699	T2914	T2912	nsubj	cells,are
R17	T224	T225	det	an,mouse
R170	T396	T393	prep	in,identified
R1700	T2915	T2914	compound	blood,cells
R1701	T2916	T2912	acomp	free,are
R1702	T2917	T2916	prep	of,free
R1703	T2918	T2919	det	any,organelles
R1704	T2919	T2917	pobj	organelles,of
R1705	T2920	T2907	punct	.,are
R1706	T2922	T2923	det	The,distribution
R1707	T2923	T2924	nsubj	distribution,was
R1708	T2925	T2924	advmod	however,was
R1709	T2926	T2924	neg	not,was
R171	T397	T396	pobj	platelets,in
R1710	T2927	T2924	acomp	homogeneous,was
R1711	T2928	T2924	prep	throughout,was
R1712	T2929	T2930	det	the,gradient
R1713	T2930	T2928	pobj	gradient,throughout
R1714	T2931	T2924	punct	.,was
R1715	T2933	T2934	nsubj	This,reflect
R1716	T2935	T2934	aux	could,reflect
R1717	T2936	T2937	det	the,binding
R1718	T2937	T2934	dobj	binding,reflect
R1719	T2938	T2937	prep	of,binding
R172	T398	T393	punct	.,identified
R1720	T2939	T2940	compound	annexin,A7
R1721	T2940	T2938	pobj	A7,of
R1722	T2941	T2937	prep	to,binding
R1723	T2942	T2943	compound	membrane,subdomains
R1724	T2943	T2941	pobj	subdomains,to
R1725	T2944	T2945	dep	that,have
R1726	T2945	T2943	relcl	have,subdomains
R1727	T2946	T2947	amod	different,composition
R1728	T2947	T2945	dobj	composition,have
R1729	T2948	T2947	nmod	lipid,composition
R173	T400	T401	prep	In,led
R1730	T2949	T2948	cc	or,lipid
R1731	T2950	T2951	compound	lipid,protein
R1732	T2951	T2948	conj	protein,lipid
R1733	T2952	T2951	punct	/,protein
R1734	T2953	T2954	mark	as,discussed
R1735	T2954	T2934	advcl	discussed,reflect
R1736	T2955	T2954	agent	by,discussed
R1737	T2956	T2955	pobj	Salzer,by
R1738	T2957	T2958	advmod	et,al.
R1739	T2958	T2956	advmod	al.,Salzer
R174	T402	T403	det	these,cells
R1740	T2959	T2960	punct	[,7
R1741	T2960	T2934	parataxis	7,reflect
R1742	T2961	T2960	punct	],7
R1743	T2962	T2934	punct	.,reflect
R1744	T2964	T2965	advmod	Likewise,exhibit
R1745	T2966	T2965	punct	", ",exhibit
R1746	T2967	T2965	nsubj	sorcin,exhibit
R1747	T2968	T2965	aux	does,exhibit
R1748	T2969	T2965	neg	not,exhibit
R1749	T2970	T2971	det	a,distribution
R175	T403	T400	pobj	cells,In
R1750	T2971	T2965	dobj	distribution,exhibit
R1751	T2972	T2971	amod	homogeneous,distribution
R1752	T2973	T2965	prep	in,exhibit
R1753	T2974	T2975	det	the,gradient
R1754	T2975	T2973	pobj	gradient,in
R1755	T2976	T2965	punct	.,exhibit
R1756	T2978	T2979	advmod	Moreover,segregates
R1757	T2980	T2979	punct	", ",segregates
R1758	T2981	T2979	nsubj	it,segregates
R1759	T2982	T2979	prep	into,segregates
R176	T404	T405	poss	its,loss
R1760	T2983	T2982	pobj	vesicles,into
R1761	T2984	T2985	dep	which,associated
R1762	T2985	T2983	relcl	associated,vesicles
R1763	T2986	T2985	auxpass	are,associated
R1764	T2987	T2985	neg	not,associated
R1765	T2988	T2985	prep	with,associated
R1766	T2989	T2990	compound	annexin,A7
R1767	T2990	T2988	pobj	A7,with
R1768	T2991	T2992	punct	(,Fig.
R1769	T2992	T2979	parataxis	Fig.,segregates
R177	T405	T401	nsubj	loss,led
R1770	T2993	T2992	nummod	3,Fig.
R1771	T2994	T2992	punct	),Fig.
R1772	T2995	T2979	punct	.,segregates
R1773	T2997	T2998	nsubj	We,tested
R1774	T2999	T2998	advmod	also,tested
R1775	T3000	T2998	dobj	platelets,tested
R1776	T3001	T2998	prep	for,tested
R1777	T3002	T3003	compound	annexin,A7
R1778	T3003	T3004	compound	A7,expression
R1779	T3004	T3001	pobj	expression,for
R178	T406	T401	prep	to,led
R1780	T3005	T2998	cc	and,tested
R1781	T3006	T2998	conj	detected,tested
R1782	T3007	T3008	det	the,isoform
R1783	T3008	T3006	dobj	isoform,detected
R1784	T3009	T3010	nummod	47,kDa
R1785	T3010	T3008	compound	kDa,isoform
R1786	T3011	T3012	punct	(,Fig.
R1787	T3012	T3006	parataxis	Fig.,detected
R1788	T3013	T3012	nummod	1,Fig.
R1789	T3014	T3012	punct	),Fig.
R179	T407	T408	det	a,velocity
R1790	T3015	T2998	punct	.,tested
R1795	T3280	T3281	det	The,lack
R1796	T3281	T3282	nsubj	lack,changes
R1797	T3283	T3281	prep	of,lack
R1798	T3284	T3285	compound	annexin,A7
R1799	T3285	T3283	pobj	A7,of
R18	T225	T223	pobj	mouse,from
R180	T408	T406	pobj	velocity,to
R1800	T3286	T3287	amod	red,cell
R1801	T3287	T3282	dobj	cell,changes
R1802	T3288	T3287	compound	blood,cell
R1803	T3289	T3287	appos	morphology,cell
R1804	T3290	T3289	cc	and,morphology
R1805	T3291	T3292	amod	osmotic,resistance
R1806	T3292	T3289	conj	resistance,morphology
R1807	T3294	T3295	mark	As,is
R1808	T3295	T3298	advcl	is,studied
R1809	T3296	T3297	compound	annexin,A7
R181	T409	T410	advmod	slightly,slower
R1810	T3297	T3295	nsubj	A7,is
R1811	T3299	T3300	det	a,component
R1812	T3300	T3295	attr	component,is
R1813	T3301	T3300	prep	of,component
R1814	T3302	T3303	amod	red,cell
R1815	T3303	T3305	compound	cell,vesicles
R1816	T3304	T3303	compound	blood,cell
R1817	T3305	T3301	pobj	vesicles,of
R1818	T3306	T3298	nsubj	we,studied
R1819	T3307	T3308	det	the,consequences
R182	T410	T408	amod	slower,velocity
R1820	T3308	T3298	dobj	consequences,studied
R1821	T3309	T3308	prep	of,consequences
R1822	T3310	T3311	det	the,loss
R1823	T3311	T3309	pobj	loss,of
R1824	T3312	T3311	prep	of,loss
R1825	T3313	T3314	det	the,protein
R1826	T3314	T3312	pobj	protein,of
R1827	T3315	T3298	prep	in,studied
R1828	T3316	T3317	det	the,mouse
R1829	T3317	T3315	pobj	mouse,in
R183	T411	T408	compound	aggregation,velocity
R1830	T3318	T3317	nmod	anxA7,mouse
R1831	T3319	T3318	punct	-,anxA7
R1832	T3320	T3318	punct	/,anxA7
R1833	T3321	T3318	punct	-,anxA7
R1834	T3322	T3298	prep	for,studied
R1835	T3323	T3324	amod	red,cell
R1836	T3324	T3322	pobj	cell,for
R1837	T3325	T3324	compound	blood,cell
R1838	T3326	T3324	appos	morphology,cell
R1839	T3327	T3326	cc	and,morphology
R184	T412	T413	dep	which,seems
R1840	T3328	T3329	amod	osmotic,resistance
R1841	T3329	T3326	conj	resistance,morphology
R1842	T3330	T3298	punct	.,studied
R1843	T3332	T3333	prep	In,revealed
R1844	T3334	T3335	det	a,smear
R1845	T3335	T3332	pobj	smear,In
R1846	T3336	T3335	amod	standard,smear
R1847	T3337	T3335	punct	',smear
R1848	T3338	T3335	compound	blood,smear
R1849	T3339	T3333	punct	',revealed
R185	T413	T408	relcl	seems,velocity
R1850	T3340	T3341	nsubj	we,note
R1851	T3341	T3333	ccomp	note,revealed
R1852	T3342	T3341	aux	did,note
R1853	T3343	T3341	neg	not,note
R1854	T3344	T3345	det	a,deformation
R1855	T3345	T3341	dobj	deformation,note
R1856	T3346	T3345	prep	of,deformation
R1857	T3347	T3348	det	the,cells
R1858	T3348	T3346	pobj	cells,of
R1859	T3349	T3333	punct	", ",revealed
R186	T414	T415	aux	to,compensated
R1860	T3350	T3333	advmod	however,revealed
R1861	T3351	T3352	amod	dark,field
R1862	T3352	T3353	compound	field,microscopy
R1863	T3353	T3333	nsubj	microscopy,revealed
R1864	T3354	T3333	dobj	changes,revealed
R1865	T3355	T3354	prep	in,changes
R1866	T3356	T3355	pobj	shape,in
R1867	T3357	T3356	cc	and,shape
R1868	T3358	T3356	conj	diameter,shape
R1869	T3359	T3356	prep	of,shape
R187	T415	T413	xcomp	compensated,seems
R1870	T3360	T3361	det	the,cells
R1871	T3361	T3359	pobj	cells,of
R1872	T3362	T3361	nmod	anxA7,cells
R1873	T3363	T3362	punct	-,anxA7
R1874	T3364	T3362	punct	/,anxA7
R1875	T3365	T3362	punct	-,anxA7
R1876	T3366	T3361	amod	red,cells
R1877	T3367	T3361	compound	blood,cells
R1878	T3368	T3333	punct	.,revealed
R1879	T3370	T3371	nsubj	They,had
R188	T416	T415	auxpass	be,compensated
R1880	T3372	T3373	det	a,diameter
R1881	T3373	T3371	dobj	diameter,had
R1882	T3374	T3375	advmod	statistically,significant
R1883	T3375	T3373	amod	significant,diameter
R1884	T3376	T3373	amod	larger,diameter
R1885	T3377	T3373	compound	cell,diameter
R1886	T3378	T3373	prep	of,diameter
R1887	T3379	T3380	nummod	6.0,μm
R1888	T3380	T3378	pobj	μm,of
R1889	T3381	T3373	prep	compared,diameter
R189	T417	T415	prep	by,compensated
R1890	T3382	T3381	prep	to,compared
R1891	T3383	T3384	amod	wild,type
R1892	T3384	T3382	pobj	type,to
R1893	T3385	T3384	prep	with,type
R1894	T3386	T3387	nummod	5.7,μm
R1895	T3387	T3385	pobj	μm,with
R1896	T3388	T3389	punct	(,40
R1897	T3389	T3373	parataxis	40,diameter
R1898	T3390	T3391	nsubj	p,0.015
R1899	T3391	T3389	ccomp	0.015,40
R19	T226	T227	nmod	annexin,A7
R190	T418	T419	det	an,number
R1900	T3392	T3391	punct	=,0.015
R1901	T3393	T3389	punct	", ",40
R1902	T3394	T3389	nsubj	n,40
R1903	T3395	T3389	punct	=,40
R1904	T3396	T3389	punct	),40
R1905	T3397	T3373	punct	", ",diameter
R1906	T3398	T3399	det	a,impression
R1907	T3399	T3373	conj	impression,diameter
R1908	T3400	T3401	advmod	remarkably,lower
R1909	T3401	T3399	amod	lower,impression
R191	T419	T417	pobj	number,by
R1910	T3402	T3399	amod	emphasized,impression
R1911	T3403	T3399	amod	central,impression
R1912	T3404	T3399	cc	and,impression
R1913	T3405	T3406	det	a,shape
R1914	T3406	T3399	conj	shape,impression
R1915	T3407	T3408	advmod	more,flat
R1916	T3408	T3406	amod	flat,shape
R1917	T3409	T3410	punct	(,Fig.
R1918	T3410	T3406	parataxis	Fig.,shape
R1919	T3411	T3410	nummod	4,Fig.
R192	T420	T419	amod	increased,number
R1920	T3412	T3410	punct	),Fig.
R1921	T3413	T3371	punct	.,had
R1922	T3415	T3416	det	No,change
R1923	T3416	T3418	nsubjpass	change,measured
R1924	T3417	T3416	amod	significant,change
R1925	T3419	T3416	prep	in,change
R1926	T3420	T3421	det	the,volume
R1927	T3421	T3419	pobj	volume,in
R1928	T3422	T3421	amod	mean,volume
R1929	T3423	T3421	amod	corpuscular,volume
R193	T421	T419	prep	of,number
R1930	T3424	T3421	punct	(,volume
R1931	T3425	T3421	appos	MCV,volume
R1932	T3426	T3418	punct	),measured
R1933	T3427	T3418	auxpass	was,measured
R1934	T3428	T3418	prep	with,measured
R1935	T3429	T3430	det	the,counter
R1936	T3430	T3428	pobj	counter,with
R1937	T3431	T3430	nmod	ADVIA,counter
R1938	T3432	T3431	nummod	120,ADVIA
R1939	T3433	T3430	compound	cell,counter
R194	T422	T421	pobj	platelets,of
R1940	T3434	T3418	punct	.,measured
R1941	T3437	T3438	nsubj	Shape,influence
R1942	T3439	T3437	cc	and,Shape
R1943	T3440	T3437	conj	MCV,Shape
R1944	T3441	T3437	prep	of,Shape
R1945	T3442	T3443	det	a,cell
R1946	T3443	T3441	pobj	cell,of
R1947	T3444	T3443	amod	red,cell
R1948	T3445	T3443	compound	blood,cell
R1949	T3446	T3438	advmod	essentially,influence
R195	T423	T401	punct	.,led
R1950	T3447	T3448	poss	its,function
R1951	T3448	T3438	dobj	function,influence
R1952	T3449	T3448	cc	and,function
R1953	T3450	T3451	amod	capillary,passage
R1954	T3451	T3448	conj	passage,function
R1955	T3452	T3438	punct	.,influence
R1956	T3454	T3455	det	The,form
R1957	T3455	T3458	nsubj	form,depends
R1958	T3456	T3455	amod	typical,form
R1959	T3457	T3455	compound	biconcave,form
R196	T425	T426	det	The,results
R1960	T3459	T3458	prep	on,depends
R1961	T3460	T3461	amod	various,influences
R1962	T3461	T3459	pobj	influences,on
R1963	T3462	T3461	prep	including,influences
R1964	T3463	T3464	det	the,composition
R1965	T3464	T3462	pobj	composition,including
R1966	T3465	T3466	compound	membrane,lipid
R1967	T3466	T3464	compound	lipid,composition
R1968	T3467	T3464	cc	and,composition
R1969	T3468	T3469	det	the,cytoskeleton
R197	T426	T427	nsubj	results,appear
R1970	T3469	T3464	conj	cytoskeleton,composition
R1971	T3470	T3469	amod	submembranous,cytoskeleton
R1972	T3471	T3472	punct	[,32
R1973	T3472	T3469	parataxis	32,cytoskeleton
R1974	T3473	T3472	punct	],32
R1975	T3474	T3475	advmod	where,play
R1976	T3475	T3469	relcl	play,cytoskeleton
R1977	T3476	T3477	compound	annexin,A7
R1978	T3477	T3475	nsubj	A7,play
R1979	T3478	T3475	aux	might,play
R198	T428	T429	aux	to,rule
R1980	T3479	T3480	det	a,role
R1981	T3480	T3475	dobj	role,play
R1982	T3481	T3458	punct	.,depends
R1983	T3483	T3484	det	The,resistance
R1984	T3484	T3486	nsubj	resistance,is
R1985	T3485	T3484	amod	osmotic,resistance
R1986	T3487	T3484	punct	", ",resistance
R1987	T3488	T3489	dep	which,is
R1988	T3489	T3484	relcl	is,resistance
R1989	T3490	T3491	det	the,resistance
R199	T429	T427	xcomp	rule,appear
R1990	T3491	T3489	attr	resistance,is
R1991	T3492	T3491	prep	towards,resistance
R1992	T3493	T3492	pobj	changes,towards
R1993	T3494	T3489	prep	in,is
R1994	T3495	T3496	det	the,strength
R1995	T3496	T3494	pobj	strength,in
R1996	T3497	T3496	amod	extracellular,strength
R1997	T3498	T3496	amod	ionic,strength
R1998	T3499	T3486	punct	", ",is
R1999	T3500	T3501	det	a,assay
R2	T209	T205	conj	expression,Function
R20	T227	T225	nmod	A7,mouse
R200	T430	T429	prt	out,rule
R2000	T3501	T3486	attr	assay,is
R2001	T3502	T3501	amod	convenient,assay
R2002	T3503	T3501	prep	for,assay
R2003	T3504	T3503	pobj	analysis,for
R2004	T3505	T3504	prep	of,analysis
R2005	T3506	T3507	det	the,integrity
R2006	T3507	T3505	pobj	integrity,of
R2007	T3508	T3509	amod	red,cell
R2008	T3509	T3507	compound	cell,integrity
R2009	T3510	T3509	compound	blood,cell
R201	T431	T432	det	an,role
R2010	T3511	T3486	punct	.,is
R2011	T3513	T3514	nsubjpass	It,measured
R2012	T3515	T3514	auxpass	is,measured
R2013	T3516	T3514	prep	as,measured
R2014	T3517	T3518	det	the,concentration
R2015	T3518	T3516	pobj	concentration,as
R2016	T3519	T3520	compound	sodium,chloride
R2017	T3520	T3518	compound	chloride,concentration
R2018	T3521	T3522	prep	at,starts
R2019	T3522	T3518	relcl	starts,concentration
R202	T432	T429	dobj	role,rule
R2020	T3523	T3521	pobj	which,at
R2021	T3524	T3525	amod	cellular,lysis
R2022	T3525	T3522	nsubj	lysis,starts
R2023	T3526	T3518	punct	(,concentration
R2024	T3527	T3528	amod	minimal,resistance
R2025	T3528	T3518	appos	resistance,concentration
R2026	T3529	T3518	punct	),concentration
R2027	T3530	T3518	prep	up,concentration
R2028	T3531	T3530	prep	to,up
R2029	T3532	T3533	det	a,lysis
R203	T433	T432	amod	important,role
R2030	T3533	T3531	pobj	lysis,to
R2031	T3534	T3533	amod	complete,lysis
R2032	T3535	T3533	punct	(,lysis
R2033	T3536	T3537	amod	maximal,resistance
R2034	T3537	T3533	appos	resistance,lysis
R2035	T3538	T3533	punct	),lysis
R2036	T3539	T3533	prep	of,lysis
R2037	T3540	T3541	det	the,cells
R2038	T3541	T3539	pobj	cells,of
R2039	T3542	T3543	punct	[,33
R204	T434	T432	prep	of,role
R2040	T3543	T3514	parataxis	33,measured
R2041	T3544	T3543	punct	],33
R2042	T3545	T3514	punct	.,measured
R2043	T3547	T3548	amod	Osmotic,resistance
R2044	T3548	T3549	nsubj	resistance,increases
R2045	T3550	T3549	prep	with,increases
R2046	T3551	T3552	amod	higher,MCV
R2047	T3552	T3550	pobj	MCV,with
R2048	T3553	T3552	punct	", ",MCV
R2049	T3554	T3555	amod	larger,area
R205	T435	T436	compound	annexin,A7
R2050	T3555	T3552	conj	area,MCV
R2051	T3556	T3555	compound	surface,area
R2052	T3557	T3555	cc	and,area
R2053	T3558	T3559	det	a,degree
R2054	T3559	T3555	conj	degree,area
R2055	T3560	T3559	amod	higher,degree
R2056	T3561	T3559	prep	of,degree
R2057	T3562	T3563	amod	cellular,metabolism
R2058	T3563	T3561	pobj	metabolism,of
R2059	T3564	T3552	acl	stabilizing,MCV
R206	T436	T434	pobj	A7,of
R2060	T3565	T3566	det	the,levels
R2061	T3566	T3564	dobj	levels,stabilizing
R2062	T3567	T3566	amod	intracellular,levels
R2063	T3568	T3566	compound	ion,levels
R2064	T3569	T3549	punct	.,increases
R2065	T3571	T3572	amod	Aged,cells
R2066	T3572	T3575	nsubj	cells,show
R2067	T3573	T3572	amod	red,cells
R2068	T3574	T3572	compound	blood,cells
R2069	T3576	T3577	det	a,MCV
R207	T437	T432	prep	in,role
R2070	T3577	T3575	dobj	MCV,show
R2071	T3578	T3577	amod	lowered,MCV
R2072	T3579	T3577	cc	and,MCV
R2073	T3580	T3581	amod	lower,resistance
R2074	T3581	T3577	conj	resistance,MCV
R2075	T3582	T3581	amod	osmotic,resistance
R2076	T3583	T3584	punct	[,33
R2077	T3584	T3575	parataxis	33,show
R2078	T3585	T3584	punct	],33
R2079	T3586	T3575	punct	.,show
R208	T438	T439	compound	membrane,fusion
R2080	T3588	T3589	amod	Similar,properties
R2081	T3589	T3590	nsubjpass	properties,observed
R2082	T3591	T3590	aux	can,observed
R2083	T3592	T3590	auxpass	be,observed
R2084	T3593	T3590	prep	in,observed
R2085	T3594	T3595	amod	red,cells
R2086	T3595	T3593	pobj	cells,in
R2087	T3596	T3595	compound	blood,cells
R2088	T3597	T3595	prep	with,cells
R2089	T3598	T3599	amod	lowered,permeability
R209	T439	T440	compound	fusion,processes
R2090	T3599	T3597	pobj	permeability,with
R2091	T3600	T3599	compound	membrane,permeability
R2092	T3601	T3590	punct	.,observed
R2093	T3603	T3604	det	The,resistance
R2094	T3604	T3606	nsubj	resistance,is
R2095	T3605	T3604	amod	osmotic,resistance
R2096	T3607	T3604	prep	of,resistance
R2097	T3608	T3609	compound	annexin,A7
R2098	T3609	T3610	npadvmod	A7,deficient
R2099	T3610	T3611	amod	deficient,cells
R21	T228	T225	amod	mutant,mouse
R210	T440	T437	pobj	processes,in
R2100	T3611	T3607	pobj	cells,of
R2101	T3612	T3611	amod	red,cells
R2102	T3613	T3611	compound	blood,cells
R2103	T3614	T3615	advmod	significantly,increased
R2104	T3615	T3606	acomp	increased,is
R2105	T3616	T3606	prep	compared,is
R2106	T3617	T3616	prep	to,compared
R2107	T3618	T3619	amod	wild,type
R2108	T3619	T3620	compound	type,cells
R2109	T3620	T3617	pobj	cells,to
R211	T441	T440	acl	occurring,processes
R2110	T3621	T3606	punct	.,is
R2111	T3623	T3624	det	The,value
R2112	T3624	T3627	nsubjpass	value,observed
R2113	T3625	T3626	amod	minimal,resistance
R2114	T3626	T3624	compound	resistance,value
R2115	T3628	T3624	prep	of,value
R2116	T3629	T3630	compound	knock,out
R2117	T3630	T3631	compound	out,cells
R2118	T3631	T3628	pobj	cells,of
R2119	T3632	T3627	auxpass	is,observed
R212	T442	T441	prep	in,occurring
R2120	T3633	T3627	prep	in,observed
R2121	T3634	T3635	det	a,solution
R2122	T3635	T3633	pobj	solution,in
R2123	T3636	T3637	nummod	0.60,%
R2124	T3637	T3635	compound	%,solution
R2125	T3638	T3635	compound	NaCl,solution
R2126	T3639	T3635	cc	versus,solution
R2127	T3640	T3641	nummod	0.65,%
R2128	T3641	T3635	conj	%,solution
R2129	T3642	T3641	prep	for,%
R213	T443	T444	amod	red,cells
R2130	T3643	T3644	amod	wild,type
R2131	T3644	T3645	compound	type,cells
R2132	T3645	T3642	pobj	cells,for
R2133	T3646	T3627	punct	.,observed
R2134	T3648	T3649	nummod	50,%
R2135	T3649	T3650	compound	%,haemolysis
R2136	T3650	T3651	nsubjpass	haemolysis,achieved
R2137	T3652	T3651	auxpass	is,achieved
R2138	T3653	T3651	prep	at,achieved
R2139	T3654	T3655	nummod	0.516,%
R214	T444	T442	pobj	cells,in
R2140	T3655	T3656	compound	%,solution
R2141	T3656	T3653	pobj	solution,at
R2142	T3657	T3656	compound	NaCl,solution
R2143	T3658	T3656	prep	for,solution
R2144	T3659	T3660	compound	knock,out
R2145	T3660	T3658	pobj	out,for
R2146	T3661	T3656	cc	versus,solution
R2147	T3662	T3663	nummod	0.564,%
R2148	T3663	T3664	compound	%,NaCl
R2149	T3664	T3656	conj	NaCl,solution
R215	T445	T444	compound	blood,cells
R2150	T3665	T3664	prep	for,NaCl
R2151	T3666	T3667	amod	wild,type
R2152	T3667	T3668	compound	type,cells
R2153	T3668	T3665	pobj	cells,for
R2154	T3669	T3670	punct	(,Fig.
R2155	T3670	T3651	parataxis	Fig.,achieved
R2156	T3671	T3672	compound	mean,values
R2157	T3672	T3670	dep	values,Fig.
R2158	T3673	T3670	punct	", ",Fig.
R2159	T3674	T3675	nsubj	p,0.00066
R216	T446	T427	punct	.,appear
R2160	T3675	T3670	ccomp	0.00066,Fig.
R2161	T3676	T3675	punct	=,0.00066
R2162	T3677	T3670	punct	", ",Fig.
R2163	T3678	T3679	nsubj	n,8
R2164	T3679	T3670	ccomp	8,Fig.
R2165	T3680	T3679	punct	=,8
R2166	T3681	T3670	punct	;,Fig.
R2167	T3682	T3670	nummod	5,Fig.
R2168	T3683	T3670	punct	),Fig.
R2169	T3684	T3651	punct	.,achieved
R217	T448	T449	advmod	Instead,involved
R2170	T3686	T3687	det	The,width
R2171	T3687	T3689	nsubj	width,is
R2172	T3688	T3687	compound	resistance,width
R2173	T3690	T3687	prep	of,width
R2174	T3691	T3692	det	the,cells
R2175	T3692	T3690	pobj	cells,of
R2176	T3693	T3694	compound	knock,out
R2177	T3694	T3692	compound	out,cells
R2178	T3695	T3696	advmod	slightly,lower
R2179	T3696	T3689	acomp	lower,is
R218	T450	T451	det	the,protein
R2180	T3697	T3698	punct	(,solution
R2181	T3698	T3696	parataxis	solution,lower
R2182	T3699	T3700	nummod	0.20,%
R2183	T3700	T3701	nmod	%,NaCl
R2184	T3701	T3698	compound	NaCl,solution
R2185	T3702	T3703	advmod	instead,of
R2186	T3703	T3700	cc	of,%
R2187	T3704	T3705	nummod	0.25,%
R2188	T3705	T3700	conj	%,%
R2189	T3706	T3698	punct	),solution
R219	T451	T449	nsubjpass	protein,involved
R2190	T3707	T3689	punct	.,is
R2191	T3709	T3710	nsubj	We,performed
R2192	T3711	T3710	advmod	also,performed
R2193	T3712	T3713	amod	direct,measurements
R2194	T3713	T3710	dobj	measurements,performed
R2195	T3714	T3713	prep	of,measurements
R2196	T3715	T3716	det	the,deformability
R2197	T3716	T3714	pobj	deformability,of
R2198	T3717	T3718	amod	red,cell
R2199	T3718	T3716	compound	cell,deformability
R22	T233	T234	compound	Annexin,A7
R220	T452	T449	aux	might,involved
R2200	T3719	T3718	compound	blood,cell
R2201	T3720	T3716	compound	membrane,deformability
R2202	T3721	T3710	punct	", ",performed
R2203	T3722	T3723	aux	to,characterize
R2204	T3723	T3710	advcl	characterize,performed
R2205	T3724	T3723	advmod	further,characterize
R2206	T3725	T3726	det	a,role
R2207	T3726	T3723	dobj	role,characterize
R2208	T3727	T3726	amod	possible,role
R2209	T3728	T3726	prep	of,role
R221	T453	T449	auxpass	be,involved
R2210	T3729	T3730	compound	annexin,A7
R2211	T3730	T3728	pobj	A7,of
R2212	T3731	T3726	prep	on,role
R2213	T3732	T3733	det	the,stability
R2214	T3733	T3731	pobj	stability,on
R2215	T3734	T3733	compound	membrane,stability
R2216	T3735	T3710	punct	.,performed
R2217	T3737	T3738	prep	With,tried
R2218	T3739	T3740	compound	micropipette,experiments
R2219	T3740	T3737	pobj	experiments,With
R222	T454	T449	prep	in,involved
R2220	T3741	T3738	nsubj	we,tried
R2221	T3742	T3743	aux	to,correlate
R2222	T3743	T3738	xcomp	correlate,tried
R2223	T3744	T3745	amod	mechanical,characteristics
R2224	T3745	T3743	dobj	characteristics,correlate
R2225	T3746	T3745	prep	of,characteristics
R2226	T3747	T3748	det	the,cells
R2227	T3748	T3746	pobj	cells,of
R2228	T3749	T3748	amod	red,cells
R2229	T3750	T3748	compound	blood,cells
R223	T455	T456	det	the,organization
R2230	T3751	T3743	prep	with,correlate
R2231	T3752	T3753	poss	their,morphology
R2232	T3753	T3751	pobj	morphology,with
R2233	T3754	T3738	punct	.,tried
R2234	T3756	T3757	nsubj	We,observe
R2235	T3758	T3757	aux	could,observe
R2236	T3759	T3757	neg	not,observe
R2237	T3760	T3761	det	any,difference
R2238	T3761	T3757	dobj	difference,observe
R2239	T3762	T3763	advmod	statistically,significant
R224	T456	T454	pobj	organization,in
R2240	T3763	T3761	amod	significant,difference
R2241	T3764	T3761	prep	of,difference
R2242	T3765	T3764	pobj	values,of
R2243	T3766	T3765	acl	describing,values
R2244	T3767	T3768	compound	membrane,rigidity
R2245	T3768	T3766	dobj	rigidity,describing
R2246	T3769	T3768	cc	and,rigidity
R2247	T3770	T3771	compound	lysis,force
R2248	T3771	T3768	conj	force,rigidity
R2249	T3772	T3757	prep	in,observe
R225	T457	T456	prep	of,organization
R2250	T3773	T3774	det	these,experiments
R2251	T3774	T3772	pobj	experiments,in
R2252	T3775	T3776	punct	(,M.
R2253	T3776	T3757	meta	M.,observe
R2254	T3777	T3776	nmod	Heil,M.
R2255	T3778	T3776	punct	", ",M.
R2256	T3779	T3776	conj	B.,M.
R2257	T3780	T3779	nmod	Hoffmann,B.
R2258	T3781	T3779	cc	and,B.
R2259	T3782	T3779	conj	R.,B.
R226	T458	T459	det	the,cytoskeleton
R2260	T3783	T3782	nmod	Merkel,R.
R2261	T3784	T3782	punct	", ",R.
R2262	T3785	T3786	amod	unpublished,results
R2263	T3786	T3782	conj	results,R.
R2264	T3787	T3786	punct	),results
R2265	T3788	T3757	punct	.,observe
R2266	T3790	T3791	advmod	However,was
R2267	T3792	T3791	punct	", ",was
R2268	T3793	T3794	det	the,distinction
R2269	T3794	T3791	nsubj	distinction,was
R227	T459	T457	pobj	cytoskeleton,of
R2270	T3795	T3794	acl	observed,distinction
R2271	T3796	T3795	prep	in,observed
R2272	T3797	T3798	det	the,experiments
R2273	T3798	T3796	pobj	experiments,in
R2274	T3799	T3800	amod	osmotic,resistance
R2275	T3800	T3798	compound	resistance,experiments
R2276	T3801	T3802	advmod	highly,significant
R2277	T3802	T3791	acomp	significant,was
R2278	T3803	T3791	cc	and,was
R2279	T3804	T3791	conj	reflects,was
R228	T460	T459	compound	membrane,cytoskeleton
R2280	T3805	T3806	det	a,value
R2281	T3806	T3804	dobj	value,reflects
R2282	T3807	T3806	amod	mean,value
R2283	T3808	T3806	prep	over,value
R2284	T3809	T3810	det	a,number
R2285	T3810	T3808	pobj	number,over
R2286	T3811	T3810	amod	high,number
R2287	T3812	T3810	prep	of,number
R2288	T3813	T3814	amod	different,cells
R2289	T3814	T3812	pobj	cells,of
R229	T461	T449	punct	.,involved
R2290	T3815	T3814	amod	red,cells
R2291	T3816	T3814	compound	blood,cells
R2292	T3817	T3791	punct	.,was
R2293	T3819	T3820	det	These,data
R2294	T3820	T3821	nsubj	data,suggest
R2295	T3822	T3823	mark	that,contributes
R2296	T3823	T3821	ccomp	contributes,suggest
R2297	T3824	T3825	compound	annexin,A7
R2298	T3825	T3823	nsubj	A7,contributes
R2299	T3826	T3823	prep	to,contributes
R23	T234	T235	nsubj	A7,is
R230	T463	T464	amod	Red,cells
R2300	T3827	T3828	det	the,shape
R2301	T3828	T3826	pobj	shape,to
R2302	T3829	T3828	prep	of,shape
R2303	T3830	T3831	amod	red,cells
R2304	T3831	T3829	pobj	cells,of
R2305	T3832	T3831	compound	blood,cells
R2306	T3833	T3828	cc	and,shape
R2307	T3834	T3835	det	the,resistance
R2308	T3835	T3828	conj	resistance,shape
R2309	T3836	T3835	amod	osmotic,resistance
R231	T464	T466	nsubj	cells,represent
R2310	T3837	T3821	punct	.,suggest
R2311	T3839	T3840	mark	As,observed
R2312	T3840	T3844	advcl	observed,do
R2313	T3841	T3840	nsubj	we,observed
R2314	T3842	T3840	aux	have,observed
R2315	T3843	T3840	neg	not,observed
R2316	T3845	T3846	det	a,binding
R2317	T3846	T3840	dobj	binding,observed
R2318	T3847	T3846	prep	of,binding
R2319	T3848	T3849	compound	annexin,A7
R232	T465	T464	compound	blood,cells
R2320	T3849	T3847	pobj	A7,of
R2321	T3850	T3846	prep	to,binding
R2322	T3851	T3852	compound	F,actin
R2323	T3852	T3850	pobj	actin,to
R2324	T3853	T3852	punct	-,actin
R2325	T3854	T3855	punct	(,shown
R2326	T3855	T3840	parataxis	shown,observed
R2327	T3856	T3855	nsubj	data,shown
R2328	T3857	T3855	neg	not,shown
R2329	T3858	T3855	punct	),shown
R233	T467	T466	aux	may,represent
R2330	T3859	T3844	nsubj	it,do
R2331	T3860	T3844	aux	could,do
R2332	T3861	T3844	advmod	so,do
R2333	T3862	T3863	preconj	either,by
R2334	T3863	T3844	prep	by,do
R2335	T3864	T3863	pobj	alteration,by
R2336	T3865	T3864	prep	of,alteration
R2337	T3866	T3867	det	the,rigidity
R2338	T3867	T3865	pobj	rigidity,of
R2339	T3868	T3867	compound	membrane,rigidity
R234	T468	T469	det	an,model
R2340	T3869	T3863	cc	and,by
R2341	T3870	T3869	punct	/,and
R2342	T3871	T3869	cc	or,and
R2343	T3872	T3863	conj	by,by
R2344	T3873	T3872	pcomp	affecting,by
R2345	T3874	T3875	det	the,homeostasis
R2346	T3875	T3873	dobj	homeostasis,affecting
R2347	T3876	T3875	compound	ion,homeostasis
R2348	T3877	T3844	punct	.,do
R2349	T4078	T4079	det	The,lack
R235	T469	T466	dobj	model,represent
R2350	T4079	T4080	nsubj	lack,affects
R2351	T4081	T4079	prep	of,lack
R2352	T4082	T4083	compound	annexin,A7
R2353	T4083	T4081	pobj	A7,of
R2354	T4084	T4085	amod	primary,haemostasis
R2355	T4085	T4080	dobj	haemostasis,affects
R2356	T4086	T4087	advmod	ex,vivo
R2357	T4087	T4080	advmod	vivo,affects
R2358	T4089	T4090	det	The,use
R2359	T4090	T4091	nsubj	use,allowed
R236	T470	T469	amod	appropriate,model
R2360	T4092	T4090	prep	of,use
R2361	T4093	T4094	det	an,counter
R2362	T4094	T4092	pobj	counter,of
R2363	T4095	T4094	amod	advanced,counter
R2364	T4096	T4094	amod	electronic,counter
R2365	T4097	T4094	compound	cell,counter
R2366	T4098	T4094	punct	/,counter
R2367	T4099	T4100	compound	flow,cytometer
R2368	T4100	T4094	appos	cytometer,counter
R2369	T4101	T4094	punct	(,counter
R237	T471	T472	aux	to,study
R2370	T4102	T4094	appos	ADVIA,counter
R2371	T4103	T4102	nummod	120,ADVIA
R2372	T4104	T4091	punct	),allowed
R2373	T4105	T4106	nsubj	us,screen
R2374	T4106	T4091	ccomp	screen,allowed
R2375	T4107	T4106	aux	to,screen
R2376	T4108	T4109	amod	other,parameters
R2377	T4109	T4106	dobj	parameters,screen
R2378	T4110	T4111	advmod	as,well
R2379	T4111	T4106	advmod	well,screen
R238	T472	T469	advcl	study,model
R2380	T4112	T4106	prep	despite,screen
R2381	T4113	T4112	pcomp	of,despite
R2382	T4114	T4115	det	the,volume
R2383	T4115	T4112	pobj	volume,despite
R2384	T4116	T4115	amod	limited,volume
R2385	T4117	T4115	amod	murine,volume
R2386	T4118	T4115	compound	blood,volume
R2387	T4119	T4091	punct	.,allowed
R2388	T4121	T4122	nsubj	We,analysed
R2389	T4123	T4122	dobj	leukocytes,analysed
R239	T473	T474	det	the,role
R2390	T4124	T4123	punct	", ",leukocytes
R2391	T4125	T4126	amod	red,cells
R2392	T4126	T4123	conj	cells,leukocytes
R2393	T4127	T4126	compound	blood,cells
R2394	T4128	T4126	punct	", ",cells
R2395	T4129	T4130	compound	haemoglobin,content
R2396	T4130	T4126	conj	content,cells
R2397	T4131	T4130	punct	", ",content
R2398	T4132	T4130	conj	haematocrit,content
R2399	T4133	T4132	punct	", ",haematocrit
R24	T236	T237	det	a,protein
R240	T474	T472	dobj	role,study
R2400	T4134	T4135	amod	mean,volume
R2401	T4135	T4132	conj	volume,haematocrit
R2402	T4136	T4135	amod	cellular,volume
R2403	T4137	T4135	punct	", ",volume
R2404	T4138	T4139	amod	mean,haemoglobin
R2405	T4139	T4135	conj	haemoglobin,volume
R2406	T4140	T4139	amod	cellular,haemoglobin
R2407	T4141	T4139	punct	", ",haemoglobin
R2408	T4142	T4143	amod	mean,haemoglobin
R2409	T4143	T4145	compound	haemoglobin,concentration
R241	T475	T474	prep	of,role
R2410	T4144	T4143	amod	cellular,haemoglobin
R2411	T4145	T4139	conj	concentration,haemoglobin
R2412	T4146	T4145	punct	", ",concentration
R2413	T4147	T4145	conj	platelets,concentration
R2414	T4148	T4147	punct	", ",platelets
R2415	T4149	T4147	conj	neutrophiles,platelets
R2416	T4150	T4149	punct	", ",neutrophiles
R2417	T4151	T4149	conj	lymphocytes,neutrophiles
R2418	T4152	T4151	punct	", ",lymphocytes
R2419	T4153	T4151	conj	monocytes,lymphocytes
R242	T476	T477	compound	annexin,A7
R2420	T4154	T4153	punct	", ",monocytes
R2421	T4155	T4153	conj	eosinophiles,monocytes
R2422	T4156	T4155	cc	and,eosinophiles
R2423	T4157	T4155	conj	basophiles,eosinophiles
R2424	T4158	T4122	punct	.,analysed
R2425	T4160	T4161	prep	In,found
R2426	T4162	T4163	det	these,tests
R2427	T4163	T4160	pobj	tests,In
R2428	T4164	T4163	prep	of,tests
R2429	T4165	T4166	amod	whole,blood
R243	T477	T475	pobj	A7,of
R2430	T4166	T4164	pobj	blood,of
R2431	T4167	T4161	nsubj	we,found
R2432	T4168	T4161	advmod	only,found
R2433	T4169	T4170	det	a,change
R2434	T4170	T4161	dobj	change,found
R2435	T4171	T4170	prep	in,change
R2436	T4172	T4173	det	the,numbers
R2437	T4173	T4171	pobj	numbers,in
R2438	T4174	T4173	compound	platelet,numbers
R2439	T4175	T4161	punct	", ",found
R244	T478	T474	prep	in,role
R2440	T4176	T4177	mark	whereas,affected
R2441	T4177	T4161	advcl	affected,found
R2442	T4178	T4179	det	all,parameters
R2443	T4179	T4177	nsubjpass	parameters,affected
R2444	T4180	T4179	amod	other,parameters
R2445	T4181	T4179	amod	haematological,parameters
R2446	T4182	T4177	auxpass	were,affected
R2447	T4183	T4177	neg	not,affected
R2448	T4184	T4161	punct	.,found
R2449	T4186	T4187	det	The,counts
R245	T479	T480	amod	cellular,processes
R2450	T4187	T4190	nsubjpass	counts,determined
R2451	T4188	T4187	amod	mean,counts
R2452	T4189	T4187	compound	platelet,counts
R2453	T4191	T4187	prep	from,counts
R2454	T4192	T4193	amod	wild,type
R2455	T4193	T4194	nmod	type,mice
R2456	T4194	T4191	pobj	mice,from
R2457	T4195	T4193	cc	and,type
R2458	T4196	T4193	conj	anxA7,type
R2459	T4197	T4196	punct	-,anxA7
R246	T480	T478	pobj	processes,in
R2460	T4198	T4196	punct	/,anxA7
R2461	T4199	T4196	punct	-,anxA7
R2462	T4200	T4194	amod	mutant,mice
R2463	T4201	T4190	auxpass	are,determined
R2464	T4202	T4190	prep	as,determined
R2465	T4203	T4204	quantmod	674,103
R2466	T4204	T4202	pobj	103,as
R2467	T4205	T4204	punct	×,103
R2468	T4206	T4207	punct	/,μl
R2469	T4207	T4204	prep	μl,103
R247	T481	T466	punct	.,represent
R2470	T4208	T4204	cc	and,103
R2471	T4209	T4210	quantmod	774,103
R2472	T4210	T4204	conj	103,103
R2473	T4211	T4210	punct	×,103
R2474	T4212	T4213	punct	/,μl
R2475	T4213	T4210	prep	μl,103
R2476	T4214	T4190	punct	", ",determined
R2477	T4215	T4190	advmod	respectively,determined
R2478	T4216	T4190	punct	.,determined
R2479	T4218	T4219	det	The,counts
R248	T485	T486	nsubj	We,demonstrated
R2480	T4219	T4221	nsubj	counts,are
R2481	T4220	T4219	compound	platelet,counts
R2482	T4222	T4219	prep	in,counts
R2483	T4223	T4224	compound	knock,out
R2484	T4224	T4225	compound	out,mice
R2485	T4225	T4222	pobj	mice,in
R2486	T4226	T4227	advmod	significantly,higher
R2487	T4227	T4221	acomp	higher,are
R2488	T4228	T4229	punct	(,14
R2489	T4229	T4227	parataxis	14,higher
R249	T487	T486	aux	have,demonstrated
R2490	T4230	T4231	nsubj	p,0.0275
R2491	T4231	T4229	ccomp	0.0275,14
R2492	T4232	T4231	punct	=,0.0275
R2493	T4233	T4229	punct	;,14
R2494	T4234	T4235	compound	n,out
R2495	T4235	T4237	nsubj	out,11
R2496	T4236	T4235	compound	knock,out
R2497	T4237	T4229	ccomp	11,14
R2498	T4238	T4237	punct	=,11
R2499	T4239	T4229	punct	", ",14
R25	T237	T235	attr	protein,is
R250	T488	T489	det	the,presence
R2500	T4240	T4241	nmod	n,type
R2501	T4241	T4229	nsubj	type,14
R2502	T4242	T4241	amod	wild,type
R2503	T4243	T4229	punct	=,14
R2504	T4244	T4229	punct	),14
R2505	T4245	T4221	punct	.,are
R2506	T4247	T4248	det	An,increase
R2507	T4248	T4249	nsubj	increase,is
R2508	T4250	T4248	prep	in,increase
R2509	T4251	T4252	compound	platelet,counts
R251	T489	T486	dobj	presence,demonstrated
R2510	T4252	T4250	pobj	counts,in
R2511	T4253	T4254	det	a,disorder
R2512	T4254	T4249	attr	disorder,is
R2513	T4255	T4256	advmod	rather,uncommon
R2514	T4256	T4254	amod	uncommon,disorder
R2515	T4257	T4249	punct	.,is
R2516	T4259	T4260	prep	In,increase
R2517	T4261	T4259	pobj	humans,In
R2518	T4262	T4260	punct	", ",increase
R2519	T4263	T4264	compound	platelet,counts
R252	T490	T489	prep	of,presence
R2520	T4264	T4260	nsubj	counts,increase
R2521	T4265	T4260	advmod	normally,increase
R2522	T4266	T4267	advmod	only,transiently
R2523	T4267	T4260	advmod	transiently,increase
R2524	T4268	T4260	prep	as,increase
R2525	T4269	T4268	pobj	in,as
R2526	T4270	T4271	amod	reactive,thrombocytosis
R2527	T4271	T4269	pobj	thrombocytosis,in
R2528	T4272	T4269	cc	and,in
R2529	T4273	T4269	conj	under,in
R253	T491	T492	preconj	both,isoforms
R2530	T4274	T4275	amod	postoperative,conditions
R2531	T4275	T4273	pobj	conditions,under
R2532	T4276	T4260	cc	or,increase
R2533	T4277	T4278	auxpass	are,increased
R2534	T4278	T4260	conj	increased,increase
R2535	T4279	T4278	advmod	largely,increased
R2536	T4280	T4278	prep	in,increased
R2537	T4281	T4282	amod	neoplastic,diseases
R2538	T4282	T4280	pobj	diseases,in
R2539	T4283	T4260	punct	.,increase
R254	T492	T490	pobj	isoforms,of
R2540	T4285	T4286	prep	By,are
R2541	T4287	T4285	pobj	contrast,By
R2542	T4288	T4286	punct	", ",are
R2543	T4289	T4290	det	the,mice
R2544	T4290	T4286	nsubj	mice,are
R2545	T4291	T4290	nmod	anxA7,mice
R2546	T4292	T4291	punct	-,anxA7
R2547	T4293	T4291	punct	/,anxA7
R2548	T4294	T4291	punct	-,anxA7
R2549	T4295	T4286	acomp	healthy,are
R255	T493	T494	compound	annexin,A7
R2550	T4296	T4295	cc	and,healthy
R2551	T4297	T4295	conj	viable,healthy
R2552	T4298	T4286	punct	.,are
R2553	T4300	T4301	advmod	Therefore,tested
R2554	T4302	T4301	nsubj	we,tested
R2555	T4303	T4304	det	the,function
R2556	T4304	T4301	dobj	function,tested
R2557	T4305	T4304	compound	platelet,function
R2558	T4306	T4301	cc	and,tested
R2559	T4307	T4301	conj	performed,tested
R256	T494	T492	compound	A7,isoforms
R2560	T4308	T4309	compound	aggregometry,measurements
R2561	T4309	T4307	dobj	measurements,performed
R2562	T4310	T4307	prep	with,performed
R2563	T4311	T4312	npadvmod	platelet,rich
R2564	T4312	T4313	amod	rich,plasma
R2565	T4313	T4310	pobj	plasma,with
R2566	T4314	T4301	punct	.,tested
R2567	T4316	T4317	compound	Platelet,activation
R2568	T4317	T4318	nsubjpass	activation,observed
R2569	T4319	T4318	auxpass	is,observed
R257	T495	T489	prep	in,presence
R2570	T4320	T4318	prep	as,observed
R2571	T4321	T4322	det	a,change
R2572	T4322	T4320	pobj	change,as
R2573	T4323	T4322	amod	morphological,change
R2574	T4324	T4322	prep	from,change
R2575	T4325	T4326	det	the,state
R2576	T4326	T4324	pobj	state,from
R2577	T4327	T4326	amod	resting,state
R2578	T4328	T4326	amod	discoid,state
R2579	T4329	T4322	prep	to,change
R258	T496	T497	amod	red,cells
R2580	T4330	T4331	amod	activated,cells
R2581	T4331	T4329	pobj	cells,to
R2582	T4332	T4331	amod	spherical,cells
R2583	T4333	T4331	prep	with,cells
R2584	T4334	T4333	pobj	pseudopods,with
R2585	T4335	T4318	punct	.,observed
R2586	T4337	T4338	det	The,changes
R2587	T4338	T4340	nsubj	changes,are
R2588	T4339	T4338	amod	morphological,changes
R2589	T4341	T4340	prep	due,are
R259	T497	T495	pobj	cells,in
R2590	T4342	T4341	pcomp	to,due
R2591	T4343	T4344	amod	cytoskeletal,rearrangements
R2592	T4344	T4341	pobj	rearrangements,due
R2593	T4345	T4340	punct	.,are
R2594	T4347	T4348	advmod	In,vitro
R2595	T4348	T4349	advmod	vitro,form
R2596	T4350	T4351	det	the,cells
R2597	T4351	T4349	nsubj	cells,form
R2598	T4352	T4351	amod	activated,cells
R2599	T4353	T4349	dobj	aggregates,form
R26	T238	T239	npadvmod	Ca2+,binding
R260	T498	T497	compound	blood,cells
R2600	T4354	T4349	advcl	recruiting,form
R2601	T4355	T4356	amod	additional,cells
R2602	T4356	T4354	dobj	cells,recruiting
R2603	T4357	T4354	prep	in,recruiting
R2604	T4358	T4359	det	the,solution
R2605	T4359	T4357	pobj	solution,in
R2606	T4360	T4361	advmod	thereby,reducing
R2607	T4361	T4354	dep	reducing,recruiting
R2608	T4362	T4363	poss	its,cloudiness
R2609	T4363	T4361	dobj	cloudiness,reducing
R261	T499	T489	cc	and,presence
R2610	T4364	T4349	punct	.,form
R2611	T4366	T4367	nsubj	Analysis,showed
R2612	T4368	T4366	prep	of,Analysis
R2613	T4369	T4370	det	the,values
R2614	T4370	T4368	pobj	values,of
R2615	T4371	T4370	compound	transmission,values
R2616	T4372	T4370	prep	of,values
R2617	T4373	T4374	compound	aggregation,curves
R2618	T4374	T4372	pobj	curves,of
R2619	T4375	T4366	prep	after,Analysis
R262	T500	T501	det	the,presence
R2620	T4376	T4377	compound	platelet,activation
R2621	T4377	T4375	pobj	activation,after
R2622	T4378	T4377	prep	by,activation
R2623	T4379	T4380	compound	ristocetin,addition
R2624	T4380	T4378	pobj	addition,by
R2625	T4381	T4382	punct	(,cofactor
R2626	T4382	T4380	parataxis	cofactor,addition
R2627	T4383	T4384	compound	von,Willebrand
R2628	T4384	T4382	compound	Willebrand,cofactor
R2629	T4385	T4382	punct	),cofactor
R263	T501	T489	conj	presence,presence
R2630	T4386	T4387	mark	that,differed
R2631	T4387	T4367	ccomp	differed,showed
R2632	T4388	T4389	det	both,curves
R2633	T4389	T4387	nsubj	curves,differed
R2634	T4390	T4387	advmod	significantly,differed
R2635	T4391	T4387	prep	at,differed
R2636	T4392	T4393	det	the,point
R2637	T4393	T4391	pobj	point,at
R2638	T4394	T4393	compound	time,point
R2639	T4395	T4393	prep	of,point
R264	T502	T501	prep	of,presence
R2640	T4396	T4397	nummod	seven,seconds
R2641	T4397	T4395	pobj	seconds,of
R2642	T4398	T4397	prep	after,seconds
R2643	T4399	T4400	compound	platelet,initiation
R2644	T4400	T4398	pobj	initiation,after
R2645	T4401	T4402	punct	(,Fig.
R2646	T4402	T4387	parataxis	Fig.,differed
R2647	T4403	T4404	nsubj	p,0.0287
R2648	T4404	T4402	ccomp	0.0287,Fig.
R2649	T4405	T4404	punct	=,0.0287
R265	T503	T504	det	the,isoform
R2650	T4406	T4402	punct	", ",Fig.
R2651	T4407	T4408	compound	n,out
R2652	T4408	T4410	nsubj	out,16
R2653	T4409	T4408	compound	knock,out
R2654	T4410	T4402	ccomp	16,Fig.
R2655	T4411	T4410	punct	=,16
R2656	T4412	T4402	punct	", ",Fig.
R2657	T4413	T4414	nmod	n,type
R2658	T4414	T4416	nsubj	type,15
R2659	T4415	T4414	amod	wild,type
R266	T504	T502	pobj	isoform,of
R2660	T4416	T4402	ccomp	15,Fig.
R2661	T4417	T4416	punct	=,15
R2662	T4418	T4402	punct	;,Fig.
R2663	T4419	T4402	nummod	6,Fig.
R2664	T4420	T4402	punct	),Fig.
R2665	T4421	T4367	punct	.,showed
R2666	T4423	T4424	compound	Annexin,A7
R2667	T4424	T4425	npadvmod	A7,deficient
R2668	T4425	T4426	amod	deficient,platelets
R2669	T4426	T4427	nsubj	platelets,showed
R267	T505	T504	amod	small,isoform
R2670	T4428	T4429	det	a,velocity
R2671	T4429	T4427	dobj	velocity,showed
R2672	T4430	T4431	advmod	slightly,lowered
R2673	T4431	T4429	amod	lowered,velocity
R2674	T4432	T4429	compound	aggregation,velocity
R2675	T4433	T4427	punct	.,showed
R2676	T4435	T4436	advmod	When,compared
R2677	T4436	T4438	advcl	compared,found
R2678	T4437	T4436	nsubj	we,compared
R2679	T4439	T4440	det	the,curves
R268	T506	T501	prep	in,presence
R2680	T4440	T4436	dobj	curves,compared
R2681	T4441	T4440	compound	aggregation,curves
R2682	T4442	T4440	prep	of,curves
R2683	T4443	T4444	amod	human,platelets
R2684	T4444	T4442	pobj	platelets,of
R2685	T4445	T4444	prep	from,platelets
R2686	T4446	T4447	det	a,donor
R2687	T4447	T4445	pobj	donor,from
R2688	T4448	T4447	amod	healthy,donor
R2689	T4449	T4436	prep	with,compared
R269	T507	T506	pobj	platelets,in
R2690	T4450	T4451	det	the,ones
R2691	T4451	T4449	pobj	ones,with
R2692	T4452	T4451	acl	obtained,ones
R2693	T4453	T4452	prep	from,obtained
R2694	T4454	T4455	det	an,individual
R2695	T4455	T4453	pobj	individual,from
R2696	T4456	T4455	prep	with,individual
R2697	T4457	T4458	det	a,defect
R2698	T4458	T4456	pobj	defect,with
R2699	T4459	T4460	nmod	von,Willebrand
R27	T239	T237	amod	binding,protein
R270	T508	T486	punct	.,demonstrated
R2700	T4460	T4461	nmod	Willebrand,factor
R2701	T4461	T4458	nmod	factor,defect
R2702	T4462	T4458	nmod	type,defect
R2703	T4463	T4462	nummod	1,type
R2704	T4464	T4438	punct	", ",found
R2705	T4465	T4438	nsubj	we,found
R2706	T4466	T4467	mark	that,was
R2707	T4467	T4438	ccomp	was,found
R2708	T4468	T4469	det	the,difference
R2709	T4469	T4467	nsubj	difference,was
R271	T510	T511	prep	In,impairs
R2710	T4470	T4469	prep	in,difference
R2711	T4471	T4472	det	the,curves
R2712	T4472	T4470	pobj	curves,in
R2713	T4473	T4474	advmod	much,more
R2714	T4474	T4475	advmod	more,pronounced
R2715	T4475	T4467	acomp	pronounced,was
R2716	T4476	T4477	mark	as,observed
R2717	T4477	T4467	advcl	observed,was
R2718	T4478	T4477	prep	in,observed
R2719	T4479	T4480	poss	our,studies
R272	T512	T513	det	both,types
R2720	T4480	T4478	pobj	studies,in
R2721	T4481	T4480	prep	of,studies
R2722	T4482	T4483	amod	healthy,platelets
R2723	T4483	T4481	pobj	platelets,of
R2724	T4484	T4483	compound	mouse,platelets
R2725	T4485	T4483	cc	and,platelets
R2726	T4486	T4487	nmod	anxA7,platelets
R2727	T4487	T4483	conj	platelets,platelets
R2728	T4488	T4486	punct	-,anxA7
R2729	T4489	T4486	punct	/,anxA7
R273	T513	T510	pobj	types,In
R2730	T4490	T4486	punct	-,anxA7
R2731	T4491	T4438	punct	.,found
R2732	T4493	T4494	advmod	Furthermore,found
R2733	T4495	T4494	nsubj	we,found
R2734	T4496	T4497	mark	that,responded
R2735	T4497	T4494	ccomp	responded,found
R2736	T4498	T4499	amod	murine,platelets
R2737	T4499	T4497	nsubj	platelets,responded
R2738	T4500	T4497	advmod	immediately,responded
R2739	T4501	T4497	prep	to,responded
R274	T514	T513	compound	cell,types
R2740	T4502	T4503	det	the,chemical
R2741	T4503	T4501	pobj	chemical,to
R2742	T4504	T4503	amod	initiating,chemical
R2743	T4505	T4506	mark	whereas,have
R2744	T4506	T4497	advcl	have,responded
R2745	T4507	T4508	amod	human,platelets
R2746	T4508	T4506	nsubj	platelets,have
R2747	T4509	T4510	det	a,phase
R2748	T4510	T4506	dobj	phase,have
R2749	T4511	T4510	compound	lag,phase
R275	T515	T516	det	the,loss
R2750	T4512	T4513	punct	[,23
R2751	T4513	T4494	parataxis	23,found
R2752	T4514	T4513	punct	],23
R2753	T4515	T4494	punct	.,found
R276	T516	T511	nsubj	loss,impairs
R2766	T5213	T5214	compound	Annexin,A7
R2767	T5214	T5215	nsubjpass	A7,thought
R2768	T5216	T5215	auxpass	is,thought
R2769	T5217	T5218	aux	to,have
R277	T517	T516	prep	of,loss
R2770	T5218	T5215	xcomp	have,thought
R2771	T5219	T5220	det	a,role
R2772	T5220	T5218	dobj	role,have
R2773	T5221	T5218	prep	in,have
R2774	T5222	T5223	compound	vesicle,fusion
R2775	T5223	T5221	pobj	fusion,in
R2776	T5224	T5221	cc	and,in
R2777	T5225	T5221	conj	in,in
R2778	T5226	T5225	pcomp	regulating,in
R2779	T5227	T5226	cc	and,regulating
R278	T518	T519	compound	annexin,A7
R2780	T5228	T5226	conj	stabilizing,regulating
R2781	T5229	T5230	compound	membrane,domains
R2782	T5230	T5228	dobj	domains,stabilizing
R2783	T5231	T5232	punct	[,1
R2784	T5232	T5215	parataxis	1,thought
R2785	T5233	T5232	punct	],1
R2786	T5234	T5215	punct	.,thought
R2787	T5236	T5237	det	The,protein
R2788	T5237	T5238	nsubjpass	protein,localized
R2789	T5238	T5241	ccomp	localized,is
R279	T519	T517	pobj	A7,of
R2790	T5239	T5238	aux	has,localized
R2791	T5240	T5238	auxpass	been,localized
R2792	T5242	T5238	prep	to,localized
R2793	T5243	T5244	det	the,membrane
R2794	T5244	T5242	pobj	membrane,to
R2795	T5245	T5244	compound	plasma,membrane
R2796	T5246	T5244	prep	including,membrane
R2797	T5247	T5248	compound	raft,subdomains
R2798	T5248	T5246	pobj	subdomains,including
R2799	T5249	T5241	punct	", ",is
R28	T240	T238	punct	-,Ca2+
R280	T520	T521	amod	cellular,functions
R2800	T5250	T5241	advmod	furthermore,is
R2801	T5251	T5241	nsubj	it,is
R2802	T5252	T5241	acomp	present,is
R2803	T5253	T5241	prep	in,is
R2804	T5254	T5255	det	the,cytosol
R2805	T5255	T5253	pobj	cytosol,in
R2806	T5256	T5257	advmod	where,found
R2807	T5257	T5255	relcl	found,cytosol
R2808	T5258	T5257	nsubjpass	it,found
R2809	T5259	T5257	auxpass	is,found
R281	T521	T511	dobj	functions,impairs
R2810	T5260	T5257	prep	on,found
R2811	T5261	T5262	amod	subcellular,structures
R2812	T5262	T5260	pobj	structures,on
R2813	T5263	T5264	npadvmod	vesicle,like
R2814	T5264	T5262	amod	like,structures
R2815	T5265	T5264	punct	-,like
R2816	T5266	T5241	punct	.,is
R2817	T5268	T5269	det	This,distribution
R2818	T5269	T5270	nsubj	distribution,appears
R2819	T5271	T5272	aux	to,be
R282	T522	T511	punct	.,impairs
R2820	T5272	T5270	xcomp	be,appears
R2821	T5273	T5272	acomp	independent,be
R2822	T5274	T5273	prep	of,independent
R2823	T5275	T5276	det	the,type
R2824	T5276	T5274	pobj	type,of
R2825	T5277	T5276	nmod	tissue,type
R2826	T5278	T5277	cc	or,tissue
R2827	T5279	T5277	conj	cell,tissue
R2828	T5280	T5276	acl	analysed,type
R2829	T5281	T5270	punct	.,appears
R283	T524	T525	det	The,defects
R2830	T5283	T5284	prep	In,increase
R2831	T5285	T5286	amod	specialized,cells
R2832	T5286	T5283	pobj	cells,In
R2833	T5287	T5285	cc	or,specialized
R2834	T5288	T5289	advmod	terminally,differentiated
R2835	T5289	T5285	conj	differentiated,specialized
R2836	T5290	T5284	punct	", ",increase
R2837	T5291	T5292	compound	expression,levels
R2838	T5292	T5284	nsubj	levels,increase
R2839	T5293	T5284	cc	and,increase
R284	T525	T526	nsubj	defects,are
R2840	T5294	T5295	advmod	frequently,expressed
R2841	T5295	T5284	conj	expressed,increase
R2842	T5296	T5297	det	the,larger
R2843	T5297	T5295	nsubjpass	larger,expressed
R2844	T5298	T5297	prep	of,larger
R2845	T5299	T5300	det	the,isoforms
R2846	T5300	T5298	pobj	isoforms,of
R2847	T5301	T5300	nummod	two,isoforms
R2848	T5302	T5295	aux	is,expressed
R2849	T5303	T5295	auxpass	being,expressed
R285	T527	T525	acl	observed,defects
R2850	T5304	T5305	punct	[,35
R2851	T5305	T5295	parataxis	35,expressed
R2852	T5306	T5305	nummod	6,35
R2853	T5307	T5305	punct	",",35
R2854	T5308	T5305	nummod	34,35
R2855	T5309	T5305	punct	",",35
R2856	T5310	T5305	punct	],35
R2857	T5311	T5284	punct	.,increase
R2858	T5313	T5314	amod	Red,cells
R2859	T5314	T5316	nsubjpass	cells,shown
R286	T528	T526	advmod	however,are
R2860	T5315	T5314	compound	blood,cells
R2861	T5317	T5314	punct	", ",cells
R2862	T5318	T5319	dep	which,thought
R2863	T5319	T5314	relcl	thought,cells
R2864	T5320	T5319	auxpass	were,thought
R2865	T5321	T5322	aux	to,be
R2866	T5322	T5319	xcomp	be,thought
R2867	T5323	T5322	acomp	devoid,be
R2868	T5324	T5323	prep	of,devoid
R2869	T5325	T5326	compound	annexin,A7
R287	T529	T526	neg	not,are
R2870	T5326	T5324	pobj	A7,of
R2871	T5327	T5316	punct	", ",shown
R2872	T5328	T5316	auxpass	were,shown
R2873	T5329	T5316	advmod	recently,shown
R2874	T5330	T5331	aux	to,harbor
R2875	T5331	T5316	xcomp	harbor,shown
R2876	T5332	T5333	det	the,isoform
R2877	T5333	T5331	dobj	isoform,harbor
R2878	T5334	T5335	nummod	47,kDa
R2879	T5335	T5333	compound	kDa,isoform
R288	T530	T526	acomp	compatible,are
R2880	T5336	T5337	advmod	together,with
R2881	T5337	T5333	prep	with,isoform
R2882	T5338	T5339	poss	its,partner
R2883	T5339	T5337	pobj	partner,with
R2884	T5340	T5339	amod	binding,partner
R2885	T5341	T5339	appos	sorcin,partner
R2886	T5342	T5343	punct	[,7
R2887	T5343	T5316	parataxis	7,shown
R2888	T5344	T5343	punct	],7
R2889	T5345	T5316	punct	.,shown
R289	T531	T530	prep	with,compatible
R2890	T5347	T5348	nsubj	We,showed
R2891	T5349	T5350	det	the,presence
R2892	T5350	T5348	dobj	presence,showed
R2893	T5351	T5350	prep	of,presence
R2894	T5352	T5353	det	the,isoform
R2895	T5353	T5351	pobj	isoform,of
R2896	T5354	T5355	nummod	51,kDa
R2897	T5355	T5353	compound	kDa,isoform
R2898	T5356	T5357	advmod	as,well
R2899	T5357	T5348	advmod	well,showed
R29	T241	T238	cc	and,Ca2+
R290	T532	T533	det	a,role
R2900	T5358	T5348	punct	", ",showed
R2901	T5359	T5348	advcl	adding,showed
R2902	T5360	T5361	amod	red,cells
R2903	T5361	T5359	dobj	cells,adding
R2904	T5362	T5361	compound	blood,cells
R2905	T5363	T5359	prep	to,adding
R2906	T5364	T5365	det	the,list
R2907	T5365	T5363	pobj	list,to
R2908	T5366	T5365	prep	of,list
R2909	T5367	T5366	pobj	cells,of
R291	T533	T531	pobj	role,with
R2910	T5368	T5369	dep	that,harbor
R2911	T5369	T5367	relcl	harbor,cells
R2912	T5370	T5371	det	this,isoform
R2913	T5371	T5369	dobj	isoform,harbor
R2914	T5372	T5348	punct	.,showed
R2915	T5374	T5375	ccomp	Salzer,showed
R2916	T5376	T5377	advmod	et,al.
R2917	T5377	T5374	advmod	al.,Salzer
R2918	T5378	T5379	punct	[,7
R2919	T5379	T5374	parataxis	7,Salzer
R292	T534	T533	amod	crucial,role
R2920	T5380	T5379	punct	],7
R2921	T5381	T5374	preconj	not,Salzer
R2922	T5382	T5381	advmod	only,not
R2923	T5383	T5374	conj	demonstrated,Salzer
R2924	T5384	T5385	det	the,presence
R2925	T5385	T5383	dobj	presence,demonstrated
R2926	T5386	T5385	prep	of,presence
R2927	T5387	T5388	compound	annexin,A7
R2928	T5388	T5386	pobj	A7,of
R2929	T5389	T5383	prep	in,demonstrated
R293	T535	T533	prep	for,role
R2930	T5390	T5391	amod	red,cells
R2931	T5391	T5389	pobj	cells,in
R2932	T5392	T5391	compound	blood,cells
R2933	T5393	T5375	punct	", ",showed
R2934	T5394	T5375	nsubj	they,showed
R2935	T5395	T5375	advmod	also,showed
R2936	T5396	T5397	mark	that,were
R2937	T5397	T5375	ccomp	were,showed
R2938	T5398	T5399	compound	annexin,A7
R2939	T5399	T5397	nsubj	A7,were
R294	T536	T537	compound	annexin,A7
R2940	T5400	T5399	cc	and,A7
R2941	T5401	T5399	conj	sorcin,A7
R2942	T5402	T5397	acomp	enriched,were
R2943	T5403	T5397	prep	in,were
R2944	T5404	T5405	compound	membrane,raft
R2945	T5405	T5406	compound	raft,domains
R2946	T5406	T5403	pobj	domains,in
R2947	T5407	T5406	prep	of,domains
R2948	T5408	T5407	pobj	nanovesicles,of
R2949	T5409	T5408	acl	formed,nanovesicles
R295	T537	T535	pobj	A7,for
R2950	T5410	T5409	prep	from,formed
R2951	T5411	T5412	amod	red,cells
R2952	T5412	T5410	pobj	cells,from
R2953	T5413	T5412	compound	blood,cells
R2954	T5414	T5415	advmod	in,vitro
R2955	T5415	T5409	advmod	vitro,formed
R2956	T5416	T5375	punct	.,showed
R2957	T5418	T5419	compound	Raft,domains
R2958	T5419	T5420	nsubjpass	domains,considered
R2959	T5421	T5420	prep	in,considered
R296	T538	T533	prep	in,role
R2960	T5422	T5421	amod	general,in
R2961	T5423	T5420	auxpass	are,considered
R2962	T5424	T5425	amod	key,players
R2963	T5425	T5420	oprd	players,considered
R2964	T5426	T5425	prep	in,players
R2965	T5427	T5428	compound	membrane,organization
R2966	T5428	T5426	pobj	organization,in
R2967	T5429	T5426	cc	and,in
R2968	T5430	T5426	conj	in,in
R2969	T5431	T5430	pcomp	mediating,in
R297	T539	T540	compound	membrane,fusion
R2970	T5432	T5433	det	the,process
R2971	T5433	T5431	dobj	process,mediating
R2972	T5434	T5433	compound	vesiculation,process
R2973	T5435	T5420	punct	.,considered
R2974	T5437	T5438	nsubj	They,result
R2975	T5439	T5438	prep	from,result
R2976	T5440	T5441	det	the,clustering
R2977	T5441	T5439	pobj	clustering,from
R2978	T5442	T5441	prep	of,clustering
R2979	T5443	T5442	pobj	sphingolipids,of
R298	T540	T541	compound	fusion,processes
R2980	T5444	T5443	cc	and,sphingolipids
R2981	T5445	T5443	conj	cholesterol,sphingolipids
R2982	T5446	T5441	prep	in,clustering
R2983	T5447	T5448	det	the,leaflet
R2984	T5448	T5446	pobj	leaflet,in
R2985	T5449	T5448	amod	outer,leaflet
R2986	T5450	T5448	prep	of,leaflet
R2987	T5451	T5452	det	the,membrane
R2988	T5452	T5450	pobj	membrane,of
R2989	T5453	T5452	acl	connected,membrane
R299	T541	T538	pobj	processes,in
R2990	T5454	T5453	prep	to,connected
R2991	T5455	T5454	pobj	phospholipids,to
R2992	T5456	T5455	cc	and,phospholipids
R2993	T5457	T5455	conj	cholesterol,phospholipids
R2994	T5458	T5453	prep	in,connected
R2995	T5459	T5460	det	the,leaflet
R2996	T5460	T5458	pobj	leaflet,in
R2997	T5461	T5460	amod	inner,leaflet
R2998	T5462	T5438	cc	and,result
R2999	T5463	T5438	conj	are,result
R3	T210	T209	cc	and,expression
R30	T242	T238	conj	phospholipid,Ca2+
R300	T542	T533	prep	in,role
R3000	T5464	T5463	acomp	enriched,are
R3001	T5465	T5463	prep	in,are
R3002	T5466	T5467	amod	special,proteins
R3003	T5467	T5465	pobj	proteins,in
R3004	T5468	T5438	punct	.,result
R3005	T5470	T5471	advmod	Biochemically,defined
R3006	T5472	T5471	nsubjpass	rafts,defined
R3007	T5473	T5471	auxpass	are,defined
R3008	T5474	T5471	prep	as,defined
R3009	T5475	T5474	pobj	membranes,as
R301	T543	T544	det	these,types
R3010	T5476	T5477	dep	that,are
R3011	T5477	T5475	relcl	are,membranes
R3012	T5478	T5477	acomp	resistant,are
R3013	T5479	T5478	prep	to,resistant
R3014	T5480	T5479	pobj	extraction,to
R3015	T5481	T5480	prep	by,extraction
R3016	T5482	T5483	amod	cold,X
R3017	T5483	T5481	pobj	X,by
R3018	T5484	T5483	compound	Triton,X
R3019	T5485	T5483	punct	-,X
R302	T544	T542	pobj	types,in
R3020	T5486	T5483	nummod	100,X
R3021	T5487	T5471	cc	and,defined
R3022	T5488	T5489	aux	can,floated
R3023	T5489	T5471	conj	floated,defined
R3024	T5490	T5489	auxpass	be,floated
R3025	T5491	T5489	prep	to,floated
R3026	T5492	T5493	amod	low,densities
R3027	T5493	T5491	pobj	densities,to
R3028	T5494	T5489	prep	in,floated
R3029	T5495	T5496	compound	sucrose,gradient
R303	T545	T544	compound	cell,types
R3030	T5496	T5497	compound	gradient,centrifugation
R3031	T5497	T5494	pobj	centrifugation,in
R3032	T5498	T5499	punct	[,36
R3033	T5499	T5489	parataxis	36,floated
R3034	T5500	T5499	punct	],36
R3035	T5501	T5471	punct	.,defined
R3036	T5503	T5504	det	The,findings
R3037	T5504	T5505	nsubj	findings,suggested
R3038	T5506	T5504	prep	of,findings
R3039	T5507	T5506	pobj	Caohuy,of
R304	T546	T526	punct	.,are
R3040	T5508	T5509	advmod	et,al.
R3041	T5509	T5507	advmod	al.,Caohuy
R3042	T5510	T5511	punct	[,37
R3043	T5511	T5507	parataxis	37,Caohuy
R3044	T5512	T5511	punct	],37
R3045	T5513	T5514	mark	that,mediates
R3046	T5514	T5504	acl	mediates,findings
R3047	T5515	T5516	compound	annexin,A7
R3048	T5516	T5514	nsubj	A7,mediates
R3049	T5517	T5518	compound	membrane,aggregation
R305	T205	T206	dep	Function,Insights
R3050	T5518	T5514	dobj	aggregation,mediates
R3051	T5519	T5505	advmod	also,suggested
R3052	T5520	T5521	mark	that,is
R3053	T5521	T5505	ccomp	is,suggested
R3054	T5522	T5523	compound	annexin,A7
R3055	T5523	T5521	nsubj	A7,is
R3056	T5524	T5525	det	an,component
R3057	T5525	T5521	attr	component,is
R3058	T5526	T5525	amod	essential,component
R3059	T5527	T5521	prep	during,is
R3060	T5528	T5527	pobj	vesiculation,during
R3061	T5529	T5505	punct	.,suggested
R3062	T5531	T5532	prep	In,reported
R3063	T5533	T5531	pobj	addition,In
R3064	T5534	T5532	nsubj	they,reported
R3065	T5535	T5536	det	a,activation
R3066	T5536	T5532	dobj	activation,reported
R3067	T5537	T5536	amod	further,activation
R3068	T5538	T5536	prep	of,activation
R3069	T5539	T5540	det	the,process
R3070	T5540	T5538	pobj	process,of
R3071	T5541	T5540	compound	vesiculation,process
R3072	T5542	T5536	prep	by,activation
R3073	T5543	T5542	pobj	GTP,by
R3074	T5544	T5532	cc	and,reported
R3075	T5545	T5532	conj	proposed,reported
R3076	T5546	T5547	mark	that,acts
R3077	T5547	T5545	ccomp	acts,proposed
R3078	T5548	T5549	compound	annexin,A7
R3079	T5549	T5547	nsubj	A7,acts
R3080	T5550	T5547	prep	as,acts
R3081	T5551	T5552	det	a,GTPase
R3082	T5552	T5550	pobj	GTPase,as
R3083	T5553	T5532	punct	.,reported
R3084	T5555	T5556	advmod	Similarly,enhanced
R3085	T5557	T5556	punct	", ",enhanced
R3086	T5558	T5559	det	the,presence
R3087	T5559	T5556	nsubj	presence,enhanced
R3088	T5560	T5559	prep	of,presence
R3089	T5561	T5560	pobj	GTP,of
R3090	T5562	T5563	det	the,secretion
R3091	T5563	T5556	dobj	secretion,enhanced
R3092	T5564	T5563	prep	of,secretion
R3093	T5565	T5564	pobj	surfactant,of
R3094	T5566	T5563	prep	by,secretion
R3095	T5567	T5568	compound	annexin,A7
R3096	T5568	T5566	pobj	A7,by
R3097	T5569	T5556	punct	.,enhanced
R3098	T5571	T5572	det	These,results
R3099	T5572	T5573	nsubjpass	results,thought
R31	T243	T239	punct	-,binding
R3100	T5574	T5573	auxpass	were,thought
R3101	T5575	T5576	aux	to,support
R3102	T5576	T5573	xcomp	support,thought
R3103	T5577	T5578	det	a,role
R3104	T5578	T5576	dobj	role,support
R3105	T5579	T5578	amod	direct,role
R3106	T5580	T5578	prep	for,role
R3107	T5581	T5582	compound	annexin,A7
R3108	T5582	T5580	pobj	A7,for
R3109	T5583	T5578	prep	in,role
R3110	T5584	T5585	amod	surfactant,secretion
R3111	T5585	T5583	pobj	secretion,in
R3112	T5586	T5573	punct	", ",thought
R3113	T5587	T5573	cc	but,thought
R3114	T5588	T5589	prep	in,separated
R3115	T5589	T5573	conj	separated,thought
R3116	T5590	T5591	det	these,studies
R3117	T5591	T5588	pobj	studies,in
R3118	T5592	T5593	compound	annexin,A7
R3119	T5593	T5594	npadvmod	A7,mediated
R3120	T5594	T5595	amod	mediated,fusion
R3121	T5595	T5589	nsubjpass	fusion,separated
R3122	T5596	T5595	compound	membrane,fusion
R3123	T5597	T5589	auxpass	was,separated
R3124	T5598	T5589	prep	from,separated
R3125	T5599	T5600	det	a,mechanism
R3126	T5600	T5598	pobj	mechanism,from
R3127	T5601	T5600	amod	second,mechanism
R3128	T5602	T5603	npadvmod	GTP,dependent
R3129	T5603	T5600	amod	dependent,mechanism
R3130	T5604	T5603	punct	-,dependent
R3131	T5605	T5606	punct	[,38
R3132	T5606	T5589	parataxis	38,separated
R3133	T5607	T5606	punct	],38
R3134	T5608	T5589	punct	.,separated
R3135	T5610	T5611	advcl	Having,tested
R3136	T5612	T5613	det	an,mouse
R3137	T5613	T5610	dobj	mouse,Having
R3138	T5614	T5615	compound	annexin,A7
R3139	T5615	T5613	compound	A7,mouse
R314	T1134	T1135	compound	Annexin,A7
R3140	T5616	T5613	compound	knockout,mouse
R3141	T5617	T5611	nsubj	we,tested
R3142	T5618	T5619	det	the,involvement
R3143	T5619	T5611	dobj	involvement,tested
R3144	T5620	T5619	prep	of,involvement
R3145	T5621	T5622	compound	annexin,A7
R3146	T5622	T5620	pobj	A7,of
R3147	T5623	T5619	prep	in,involvement
R3148	T5624	T5623	pobj	budding,in
R3149	T5625	T5624	prep	of,budding
R315	T1135	T1136	nsubj	A7,is
R3150	T5626	T5627	amod	red,cell
R3151	T5627	T5629	compound	cell,vesicles
R3152	T5628	T5627	compound	blood,cell
R3153	T5629	T5625	pobj	vesicles,of
R3154	T5630	T5611	punct	.,tested
R3155	T5632	T5633	poss	Our,data
R3156	T5633	T5634	nsubj	data,suggest
R3157	T5635	T5636	mark	that,is
R3158	T5636	T5634	ccomp	is,suggest
R3159	T5637	T5638	det	the,efficiency
R316	T1137	T1138	det	a,protein
R3160	T5638	T5636	nsubj	efficiency,is
R3161	T5639	T5638	prep	of,efficiency
R3162	T5640	T5641	nmod	micro,nanovesicle
R3163	T5641	T5644	compound	nanovesicle,formation
R3164	T5642	T5641	punct	-,nanovesicle
R3165	T5643	T5641	cc	and,nanovesicle
R3166	T5644	T5639	pobj	formation,of
R3167	T5645	T5636	neg	not,is
R3168	T5646	T5647	advmod	significantly,different
R3169	T5647	T5636	acomp	different,is
R317	T1138	T1136	attr	protein,is
R3170	T5648	T5636	prep	in,is
R3171	T5649	T5650	amod	wild,type
R3172	T5650	T5651	nmod	type,cells
R3173	T5651	T5648	pobj	cells,in
R3174	T5652	T5650	cc	and,type
R3175	T5653	T5650	conj	mutant,type
R3176	T5654	T5651	amod	red,cells
R3177	T5655	T5651	compound	blood,cells
R3178	T5656	T5634	punct	.,suggest
R3179	T5658	T5659	advmod	Likewise,were
R318	T1139	T1140	npadvmod	Ca2+,binding
R3180	T5660	T5659	punct	", ",were
R3181	T5661	T5662	compound	Dictyostelium,discoideum
R3182	T5662	T5663	compound	discoideum,cells
R3183	T5663	T5659	nsubj	cells,were
R3184	T5664	T5663	punct	", ",cells
R3185	T5665	T5666	prep	in,was
R3186	T5666	T5663	relcl	was,cells
R3187	T5667	T5665	pobj	which,in
R3188	T5668	T5669	det	the,gene
R3189	T5669	T5666	nsubj	gene,was
R319	T1140	T1138	amod	binding,protein
R3190	T5670	T5669	amod	single,gene
R3191	T5671	T5669	compound	annexin,gene
R3192	T5672	T5666	acomp	inactivated,was
R3193	T5673	T5659	punct	", ",were
R3194	T5674	T5659	neg	not,were
R3195	T5675	T5659	acomp	impaired,were
R3196	T5676	T5675	prep	in,impaired
R3197	T5677	T5676	pobj	processes,in
R3198	T5678	T5677	acl	requiring,processes
R3199	T5679	T5680	compound	membrane,fusion
R32	T244	T237	acl	expressed,protein
R320	T1141	T1139	punct	-,Ca2+
R3200	T5680	T5678	dobj	fusion,requiring
R3201	T5681	T5682	punct	[,39
R3202	T5682	T5659	parataxis	39,were
R3203	T5683	T5682	punct	[,39
R3204	T5684	T5682	punct	],39
R3205	T5685	T5682	punct	;,39
R3206	T5686	T5682	cc	and,39
R3207	T5687	T5682	conj	data,39
R3208	T5688	T5687	amod	unpublished,data
R3209	T5689	T5687	punct	],data
R321	T1142	T1139	cc	and,Ca2+
R3210	T5690	T5659	punct	.,were
R3211	T5692	T5693	nsubj	It,be
R3212	T5694	T5693	aux	might,be
R3213	T5695	T5693	advmod	well,be
R3214	T5696	T5697	mark	that,turn
R3215	T5697	T5693	ccomp	turn,be
R3216	T5698	T5699	det	the,fusion
R3217	T5699	T5697	nsubj	fusion,turn
R3218	T5700	T5699	amod	observed,fusion
R3219	T5701	T5699	prep	of,fusion
R322	T1143	T1139	conj	phospholipid,Ca2+
R3220	T5702	T5703	compound	phospholipid,layers
R3221	T5703	T5701	pobj	layers,of
R3222	T5704	T5699	prep	by,fusion
R3223	T5705	T5706	compound	annexin,A7
R3224	T5706	T5704	pobj	A7,by
R3225	T5707	T5699	prep	in,fusion
R3226	T5708	T5709	det	the,presence
R3227	T5709	T5707	pobj	presence,in
R3228	T5710	T5709	prep	of,presence
R3229	T5711	T5712	amod	high,concentrations
R323	T1144	T1140	punct	-,binding
R3230	T5712	T5710	pobj	concentrations,of
R3231	T5713	T5712	compound	Ca2+,concentrations
R3232	T5714	T5697	aux	may,turn
R3233	T5715	T5697	prt	out,turn
R3234	T5716	T5717	aux	to,be
R3235	T5717	T5697	xcomp	be,turn
R3236	T5718	T5719	det	an,effect
R3237	T5719	T5717	attr	effect,be
R3238	T5720	T5721	advmod	in,vitro
R3239	T5721	T5719	amod	vitro,effect
R324	T1145	T1138	punct	", ",protein
R3240	T5722	T5693	punct	.,be
R3241	T5724	T5725	advmod	Similarly,be
R3242	T5726	T5725	punct	", ",be
R3243	T5727	T5728	det	the,abundance
R3244	T5728	T5725	nsubj	abundance,be
R3245	T5729	T5728	amod	high,abundance
R3246	T5730	T5728	prep	of,abundance
R3247	T5731	T5732	compound	annexin,A7
R3248	T5732	T5730	pobj	A7,of
R3249	T5733	T5728	prep	in,abundance
R325	T1146	T1147	dep	which,isolated
R3250	T5734	T5735	nmod	micro,nanovesicles
R3251	T5735	T5733	pobj	nanovesicles,in
R3252	T5736	T5735	punct	-,nanovesicles
R3253	T5737	T5735	cc	and,nanovesicles
R3254	T5738	T5725	aux	might,be
R3255	T5739	T5725	acomp	fortuitous,be
R3256	T5740	T5741	mark	as,appears
R3257	T5741	T5725	advcl	appears,be
R3258	T5742	T5743	det	a,percentage
R3259	T5743	T5741	nsubj	percentage,appears
R326	T1147	T1138	relcl	isolated,protein
R3260	T5744	T5743	amod	significant,percentage
R3261	T5745	T5743	prep	of,percentage
R3262	T5746	T5747	det	the,membrane
R3263	T5747	T5745	pobj	membrane,of
R3264	T5748	T5749	amod	red,cell
R3265	T5749	T5747	compound	cell,membrane
R3266	T5750	T5749	compound	blood,cell
R3267	T5751	T5747	punct	", ",membrane
R3268	T5752	T5753	prep	with,associates
R3269	T5753	T5747	relcl	associates,membrane
R327	T1148	T1147	auxpass	was,isolated
R3270	T5754	T5752	pobj	which,with
R3271	T5755	T5756	compound	annexin,A7
R3272	T5756	T5753	nsubj	A7,associates
R3273	T5757	T5758	advmod	when,rise
R3274	T5758	T5753	advcl	rise,associates
R3275	T5759	T5760	det	the,levels
R3276	T5760	T5758	nsubj	levels,rise
R3277	T5761	T5760	compound	Ca2+,levels
R3278	T5762	T5741	punct	", ",appears
R3279	T5763	T5764	aux	to,involved
R328	T1149	T1147	prep	as,isolated
R3280	T5764	T5741	xcomp	involved,appears
R3281	T5765	T5764	auxpass	be,involved
R3282	T5766	T5764	prep	in,involved
R3283	T5767	T5768	det	the,formation
R3284	T5768	T5766	pobj	formation,in
R3285	T5769	T5768	prep	of,formation
R3286	T5770	T5769	pobj	exovesicles,of
R3287	T5771	T5725	punct	.,be
R3288	T5773	T5774	advmod	Furthermore,indicates
R3289	T5775	T5774	punct	", ",indicates
R329	T1150	T1151	det	the,agent
R3290	T5776	T5777	det	the,distribution
R3291	T5777	T5774	nsubj	distribution,indicates
R3292	T5778	T5777	prep	of,distribution
R3293	T5779	T5780	det	both,isoforms
R3294	T5780	T5778	pobj	isoforms,of
R3295	T5781	T5782	compound	annexin,A7
R3296	T5782	T5780	compound	A7,isoforms
R3297	T5783	T5777	prep	in,distribution
R3298	T5784	T5783	pobj	gradients,in
R3299	T5785	T5784	prep	of,gradients
R33	T245	T244	prep	as,expressed
R330	T1151	T1149	pobj	agent,as
R3300	T5786	T5787	amod	red,cells
R3301	T5787	T5785	pobj	cells,of
R3302	T5788	T5787	compound	blood,cells
R3303	T5789	T5790	det	a,function
R3304	T5790	T5774	dobj	function,indicates
R3305	T5791	T5792	dep	which,differs
R3306	T5792	T5790	relcl	differs,function
R3307	T5793	T5792	prep	from,differs
R3308	T5794	T5793	pcomp	organizing,from
R3309	T5795	T5796	amod	subcellular,membranes
R331	T1152	T1153	dep	that,mediated
R3310	T5796	T5794	dobj	membranes,organizing
R3311	T5797	T5796	cc	or,membranes
R3312	T5798	T5799	compound	membrane,pathways
R3313	T5799	T5796	conj	pathways,membranes
R3314	T5800	T5774	punct	.,indicates
R3315	T5802	T5803	mark	Whereas,associated
R3316	T5803	T5808	advcl	associated,distributes
R3317	T5804	T5805	compound	annexin,A2
R3318	T5805	T5803	nsubjpass	A2,associated
R3319	T5806	T5803	auxpass	is,associated
R332	T1153	T1151	relcl	mediated,agent
R3320	T5807	T5803	advmod	tightly,associated
R3321	T5809	T5803	prep	with,associated
R3322	T5810	T5809	pobj	endosomes,with
R3323	T5811	T5803	cc	and,associated
R3324	T5812	T5813	aux	may,have
R3325	T5813	T5803	conj	have,associated
R3326	T5814	T5813	dobj	functions,have
R3327	T5815	T5813	prep	in,have
R3328	T5816	T5817	compound	membrane,fusion
R3329	T5817	T5815	pobj	fusion,in
R333	T1154	T1153	dobj	aggregation,mediated
R3330	T5818	T5817	cc	and,fusion
R3331	T5819	T5820	det	the,pathway
R3332	T5820	T5817	conj	pathway,fusion
R3333	T5821	T5820	amod	endosomal,pathway
R3334	T5822	T5808	punct	", ",distributes
R3335	T5823	T5824	compound	annexin,A7
R3336	T5824	T5808	nsubj	A7,distributes
R3337	T5825	T5808	prep	with,distributes
R3338	T5826	T5827	compound	plasma,membrane
R3339	T5827	T5828	compound	membrane,domains
R334	T1155	T1154	prep	of,aggregation
R3340	T5828	T5825	pobj	domains,with
R3341	T5829	T5828	compound	raft,domains
R3342	T5830	T5828	prep	of,domains
R3343	T5831	T5832	amod	different,types
R3344	T5832	T5830	pobj	types,of
R3345	T5833	T5832	cc	and,types
R3346	T5834	T5832	conj	densities,types
R3347	T5835	T5808	punct	.,distributes
R3348	T5837	T5838	advmod	Interestingly,lowered
R3349	T5839	T5838	punct	", ",lowered
R335	T1156	T1157	compound	chromaffin,granules
R3350	T5840	T5838	prep	in,lowered
R3351	T5841	T5840	pobj	vesicles,in
R3352	T5842	T5841	prep	of,vesicles
R3353	T5843	T5844	amod	red,cells
R3354	T5844	T5842	pobj	cells,of
R3355	T5845	T5844	compound	blood,cells
R3356	T5846	T5844	acl	lacking,cells
R3357	T5847	T5848	compound	annexin,A7
R3358	T5848	T5846	dobj	A7,lacking
R3359	T5849	T5850	det	the,amount
R336	T1157	T1155	pobj	granules,of
R3360	T5850	T5838	nsubjpass	amount,lowered
R3361	T5851	T5850	prep	of,amount
R3362	T5852	T5851	pobj	sorcin,of
R3363	T5853	T5838	auxpass	is,lowered
R3364	T5854	T5838	punct	", ",lowered
R3365	T5855	T5838	advcl	indicating,lowered
R3366	T5856	T5857	mark	that,affected
R3367	T5857	T5855	ccomp	affected,indicating
R3368	T5858	T5859	poss	its,localisation
R3369	T5859	T5857	nsubjpass	localisation,affected
R337	T1158	T1154	cc	and,aggregation
R3370	T5860	T5857	auxpass	is,affected
R3371	T5861	T5857	agent	by,affected
R3372	T5862	T5863	det	the,loss
R3373	T5863	T5861	pobj	loss,by
R3374	T5864	T5863	prep	of,loss
R3375	T5865	T5866	compound	annexin,A7
R3376	T5866	T5864	pobj	A7,of
R3377	T5867	T5857	cc	or,affected
R3378	T5868	T5869	poss	its,expression
R3379	T5869	T5870	nsubjpass	expression,reduced
R338	T1159	T1154	conj	fusion,aggregation
R3380	T5870	T5857	conj	reduced,affected
R3381	T5871	T5869	prep	during,expression
R3382	T5872	T5873	amod	red,cell
R3383	T5873	T5875	compound	cell,development
R3384	T5874	T5873	compound	blood,cell
R3385	T5875	T5871	pobj	development,during
R3386	T5876	T5870	auxpass	was,reduced
R3387	T5877	T5838	punct	.,lowered
R3388	T5879	T5880	aux	Having,excluded
R3389	T5880	T5881	advcl	excluded,act
R339	T1160	T1159	prep	of,fusion
R3390	T5882	T5883	det	a,role
R3391	T5883	T5880	dobj	role,excluded
R3392	T5884	T5883	amod	direct,role
R3393	T5885	T5883	prep	in,role
R3394	T5886	T5887	compound	vesicle,formation
R3395	T5887	T5885	pobj	formation,in
R3396	T5888	T5887	cc	and,formation
R3397	T5889	T5890	compound	membrane,fusion
R3398	T5890	T5887	conj	fusion,formation
R3399	T5891	T5881	punct	", ",act
R34	T246	T247	det	a,isoform
R340	T1161	T1162	compound	phospholipid,membranes
R3400	T5892	T5893	compound	annexin,A7
R3401	T5893	T5881	nsubj	A7,act
R3402	T5894	T5881	aux	might,act
R3403	T5895	T5881	prep	by,act
R3404	T5896	T5897	poss	its,property
R3405	T5897	T5895	pobj	property,by
R3406	T5898	T5897	prep	as,property
R3407	T5899	T5900	npadvmod	Ca2+,binding
R3408	T5900	T5902	amod	binding,protein
R3409	T5901	T5900	punct	-,binding
R341	T1162	T1160	pobj	membranes,of
R3410	T5902	T5898	pobj	protein,as
R3411	T5903	T5904	punct	[,17
R3412	T5904	T5881	parataxis	17,act
R3413	T5905	T5904	punct	],17
R3414	T5906	T5881	punct	.,act
R3415	T5908	T5909	amod	Previous,results
R3416	T5909	T5910	nsubjpass	results,explained
R3417	T5911	T5912	prep	in,shown
R3418	T5912	T5909	relcl	shown,results
R3419	T5913	T5911	pobj	which,in
R342	T1163	T1154	prep	in,aggregation
R3420	T5914	T5915	compound	annexin,A7
R3421	T5915	T5912	nsubjpass	A7,shown
R3422	T5916	T5912	auxpass	was,shown
R3423	T5917	T5918	aux	to,play
R3424	T5918	T5912	xcomp	play,shown
R3425	T5919	T5920	det	a,role
R3426	T5920	T5918	dobj	role,play
R3427	T5921	T5918	prep	in,play
R3428	T5922	T5923	compound	membrane,aggregation
R3429	T5923	T5921	pobj	aggregation,in
R343	T1164	T1165	det	the,presence
R3430	T5924	T5910	aux	might,explained
R3431	T5925	T5910	auxpass	be,explained
R3432	T5926	T5910	agent	by,explained
R3433	T5927	T5928	det	a,function
R3434	T5928	T5926	pobj	function,by
R3435	T5929	T5928	amod	supportive,function
R3436	T5930	T5928	prep	of,function
R3437	T5931	T5932	compound	annexin,A7
R3438	T5932	T5930	pobj	A7,of
R3439	T5933	T5934	mark	in,interferes
R344	T1165	T1163	pobj	presence,in
R3440	T5934	T5910	advcl	interferes,explained
R3441	T5935	T5934	mark	that,interferes
R3442	T5936	T5934	nsubj	it,interferes
R3443	T5937	T5934	prep	with,interferes
R3444	T5938	T5939	det	the,homeostasis
R3445	T5939	T5937	pobj	homeostasis,with
R3446	T5940	T5939	amod	local,homeostasis
R3447	T5941	T5939	compound	Ca2+,homeostasis
R3448	T5942	T5943	advmod	thus,influencing
R3449	T5943	T5934	advcl	influencing,interferes
R345	T1166	T1165	prep	of,presence
R3450	T5944	T5945	compound	membrane,organization
R3451	T5945	T5943	dobj	organization,influencing
R3452	T5946	T5910	punct	.,explained
R3453	T5948	T5949	mark	As,belong
R3454	T5949	T5952	advcl	belong,expected
R3455	T5950	T5951	det	the,annexins
R3456	T5951	T5949	nsubj	annexins,belong
R3457	T5953	T5949	prep	to,belong
R3458	T5954	T5955	det	a,family
R3459	T5955	T5953	pobj	family,to
R346	T1167	T1166	pobj	Ca2+,of
R3460	T5956	T5957	advmod	wide,spread
R3461	T5957	T5955	amod	spread,family
R3462	T5958	T5957	cc	and,spread
R3463	T5959	T5960	advmod	evolutionarily,conserved
R3464	T5960	T5957	conj	conserved,spread
R3465	T5961	T5955	compound	protein,family
R3466	T5962	T5952	punct	", ",expected
R3467	T5963	T5964	npadvmod	redundant,identical
R3468	T5964	T5968	amod	identical,functions
R3469	T5965	T5964	punct	", ",identical
R347	T1168	T1136	punct	.,is
R3470	T5966	T5964	cc	but,identical
R3471	T5967	T5966	neg	not,but
R3472	T5968	T5952	nsubjpass	functions,expected
R3473	T5969	T5952	auxpass	are,expected
R3474	T5970	T5952	punct	.,expected
R3475	T5972	T5973	advmod	However,described
R3476	T5974	T5973	punct	", ",described
R3477	T5975	T5976	advmod	so,far
R3478	T5976	T5973	advmod	far,described
R3479	T5977	T5978	det	no,members
R348	T1170	T1171	det	This,activity
R3480	T5978	T5973	nsubjpass	members,described
R3481	T5979	T5978	amod	other,members
R3482	T5980	T5978	prep	of,members
R3483	T5981	T5982	det	the,family
R3484	T5982	T5980	pobj	family,of
R3485	T5983	T5982	compound	annexin,family
R3486	T5984	T5982	punct	", ",family
R3487	T5985	T5986	dep	which,substitute
R3488	T5986	T5982	relcl	substitute,family
R3489	T5987	T5986	aux	may,substitute
R349	T1171	T1172	nsubj	activity,led
R3490	T5988	T5986	prep	for,substitute
R3491	T5989	T5990	compound	annexin,A7
R3492	T5990	T5988	pobj	A7,for
R3493	T5991	T5973	punct	", ",described
R3494	T5992	T5973	aux	have,described
R3495	T5993	T5973	auxpass	been,described
R3496	T5994	T5973	prep	in,described
R3497	T5995	T5996	amod	red,cells
R3498	T5996	T5994	pobj	cells,in
R3499	T5997	T5996	compound	blood,cells
R35	T247	T245	pobj	isoform,as
R350	T1173	T1172	prep	to,led
R3500	T5998	T5973	punct	.,described
R3501	T6000	T6001	det	The,basis
R3502	T6001	T6003	nsubj	basis,are
R3503	T6002	T6001	amod	structural,basis
R3504	T6004	T6001	prep	for,basis
R3505	T6005	T6006	det	the,elasticy
R3506	T6006	T6004	pobj	elasticy,for
R3507	T6007	T6006	prep	of,elasticy
R3508	T6008	T6009	amod	red,cells
R3509	T6009	T6007	pobj	cells,of
R351	T1174	T1175	det	the,proposal
R3510	T6010	T6009	compound	blood,cells
R3511	T6011	T6009	prep	in,cells
R3512	T6012	T6011	pobj	circulation,in
R3513	T6013	T6014	amod	long,range
R3514	T6014	T6015	nmod	range,functions
R3515	T6015	T6003	attr	functions,are
R3516	T6016	T6015	amod	molecular,functions
R3517	T6017	T6015	prep	of,functions
R3518	T6018	T6019	det	the,membrane
R3519	T6019	T6017	pobj	membrane,of
R352	T1175	T1173	pobj	proposal,to
R3520	T6020	T6019	compound	plasma,membrane
R3521	T6021	T6019	cc	and,membrane
R3522	T6022	T6023	det	the,part
R3523	T6023	T6019	conj	part,membrane
R3524	T6024	T6025	npadvmod	membrane,associated
R3525	T6025	T6023	amod	associated,part
R3526	T6026	T6023	prep	of,part
R3527	T6027	T6028	det	the,cytoskeleton
R3528	T6028	T6026	pobj	cytoskeleton,of
R3529	T6029	T6030	punct	[,40
R353	T1176	T1175	prep	of,proposal
R3530	T6030	T6003	parataxis	40,are
R3531	T6031	T6032	punct	-,43
R3532	T6032	T6030	prep	43,40
R3533	T6033	T6030	punct	],40
R3534	T6034	T6003	punct	.,are
R3535	T6036	T6037	amod	Major,constituents
R3536	T6037	T6038	nsubj	constituents,are
R3537	T6039	T6037	prep	of,constituents
R3538	T6040	T6041	det	the,skeleton
R3539	T6041	T6039	pobj	skeleton,of
R354	T1177	T1178	compound	annexin,A7
R3540	T6042	T6041	compound	membrane,skeleton
R3541	T6043	T6044	compound	spectrin,tetramers
R3542	T6044	T6038	attr	tetramers,are
R3543	T6045	T6044	acl	linked,tetramers
R3544	T6046	T6045	advmod	together,linked
R3545	T6047	T6045	agent	by,linked
R3546	T6048	T6049	amod	short,filaments
R3547	T6049	T6047	pobj	filaments,by
R3548	T6050	T6049	compound	actin,filaments
R3549	T6051	T6049	cc	and,filaments
R355	T1178	T1179	poss	A7,involvement
R3550	T6052	T6053	amod	several,proteins
R3551	T6053	T6049	conj	proteins,filaments
R3552	T6054	T6053	amod	other,proteins
R3553	T6055	T6053	acl	covering,proteins
R3554	T6056	T6057	det	the,surface
R3555	T6057	T6055	dobj	surface,covering
R3556	T6058	T6057	amod	entire,surface
R3557	T6059	T6057	amod	cytoplasmic,surface
R3558	T6060	T6057	prep	of,surface
R3559	T6061	T6062	det	the,membrane
R356	T1179	T1176	pobj	involvement,of
R3560	T6062	T6060	pobj	membrane,of
R3561	T6063	T6064	amod	red,cell
R3562	T6064	T6062	compound	cell,membrane
R3563	T6065	T6064	compound	blood,cell
R3564	T6066	T6067	punct	[,44
R3565	T6067	T6038	parataxis	44,are
R3566	T6068	T6067	punct	],44
R3567	T6069	T6038	punct	.,are
R3568	T6071	T6072	compound	Membrane,cholesterol
R3569	T6072	T6073	nsubj	cholesterol,diminishes
R357	T1180	T1178	case	's,A7
R3570	T6074	T6075	amod	red,cell
R3571	T6075	T6077	compound	cell,haemolysis
R3572	T6076	T6075	compound	blood,cell
R3573	T6077	T6073	dobj	haemolysis,diminishes
R3574	T6078	T6077	prep	by,haemolysis
R3575	T6079	T6080	det	the,complex
R3576	T6080	T6078	pobj	complex,by
R3577	T6081	T6080	compound	complement,complex
R3578	T6082	T6073	punct	", ",diminishes
R3579	T6083	T6084	mark	whereas,abrogates
R358	T1181	T1179	prep	in,involvement
R3580	T6084	T6073	advcl	abrogates,diminishes
R3581	T6085	T6084	nsubj	depletion,abrogates
R3582	T6086	T6085	prep	of,depletion
R3583	T6087	T6088	compound	raft,cholesterol
R3584	T6088	T6086	pobj	cholesterol,of
R3585	T6089	T6084	dobj	association,abrogates
R3586	T6090	T6089	prep	of,association
R3587	T6091	T6092	det	all,proteins
R3588	T6092	T6090	pobj	proteins,of
R3589	T6093	T6092	compound	raft,proteins
R359	T1182	T1183	det	the,secretion
R3590	T6094	T6084	prep	with,abrogates
R3591	T6095	T6096	det	no,effect
R3592	T6132	T6130	dobj	resistance,have
R3593	T6096	T6094	pobj	effect,with
R3594	T6097	T6096	amod	significant,effect
R3595	T6098	T6096	prep	on,effect
R3596	T6133	T6132	amod	higher,resistance
R3597	T6099	T6098	pobj	areas,on
R3598	T6134	T6132	amod	osmotic,resistance
R3599	T6135	T6124	punct	.,show
R36	T248	T249	nummod	47,kDa
R360	T1183	T1181	pobj	secretion,in
R3600	T6100	T6099	prep	in,areas
R3601	T6137	T6138	det	The,presence
R3602	T6101	T6102	det	the,rest
R3603	T6138	T6139	nsubj	presence,alter
R3604	T6140	T6138	prep	of,presence
R3605	T6102	T6100	pobj	rest,in
R3606	T6141	T6142	compound	annexin,A7
R3607	T6142	T6140	pobj	A7,of
R3608	T6143	T6139	aux	may,alter
R3609	T6103	T6102	prep	of,rest
R361	T1184	T1183	amod	exocytotic,secretion
R3610	T6144	T6145	det	the,flexibility
R3611	T6145	T6139	dobj	flexibility,alter
R3612	T6104	T6105	det	the,membrane
R3613	T6146	T6145	compound	membrane,flexibility
R3614	T6147	T6148	mark	in,supports
R3615	T6148	T6139	advcl	supports,alter
R3616	T6105	T6103	pobj	membrane,of
R3617	T6149	T6148	mark	that,supports
R3618	T6150	T6148	nsubj	it,supports
R3619	T6106	T6105	compound	cell,membrane
R362	T1185	T1183	prep	of,secretion
R3620	T6151	T6152	det	the,shape
R3621	T6152	T6148	dobj	shape,supports
R3622	T6153	T6152	amod	typical,shape
R3623	T6107	T6099	cc	and,areas
R3624	T6154	T6152	compound	biconcave,shape
R3625	T6155	T6152	compound	membrane,shape
R3626	T6108	T6099	conj	deformability,areas
R3627	T6156	T6148	cc	and,supports
R3628	T6157	T6148	conj	leads,supports
R3629	T6158	T6157	prep	to,leads
R363	T1186	T1185	pobj	catecholamines,of
R3630	T6159	T6160	det	a,resistance
R3631	T6109	T6110	punct	[,46
R3632	T6160	T6158	pobj	resistance,to
R3633	T6161	T6160	amod	lower,resistance
R3634	T6162	T6160	amod	minimal,resistance
R3635	T6163	T6160	amod	osmotic,resistance
R3636	T6110	T6073	parataxis	46,diminishes
R3637	T6164	T6139	punct	.,alter
R3638	T6166	T6167	compound	Annexin,A7
R3639	T6167	T6168	nsubj	A7,appear
R364	T1187	T1188	punct	[,1
R3640	T6111	T6110	nummod	45,46
R3641	T6169	T6168	aux	does,appear
R3642	T6170	T6168	neg	not,appear
R3643	T6112	T6110	punct	",",46
R3644	T6171	T6172	aux	to,have
R3645	T6172	T6168	xcomp	have,appear
R3646	T6173	T6174	det	an,influence
R3647	T6113	T6110	punct	],46
R3648	T6174	T6172	dobj	influence,have
R3649	T6175	T6174	prep	on,influence
R365	T1188	T1172	parataxis	1,led
R3650	T6114	T6073	punct	.,diminishes
R3651	T6176	T6177	compound	plasma,membrane
R3652	T6177	T6178	compound	membrane,integrity
R3653	T6178	T6175	pobj	integrity,on
R3654	T6116	T6117	nmod	Annexin,A7
R3655	T6179	T6178	appos	itself,integrity
R3656	T6180	T6181	mark	as,stays
R3657	T6181	T6172	advcl	stays,have
R3658	T6182	T6183	det	the,value
R3659	T6117	T6118	nmod	A7,cells
R366	T1189	T1188	punct	],1
R3660	T6183	T6181	nsubj	value,stays
R3661	T6184	T6183	amod	maximal,value
R3662	T6185	T6186	amod	osmotic,resistance
R3663	T6118	T6124	nsubj	cells,show
R3664	T6186	T6183	compound	resistance,value
R3665	T6187	T6181	acomp	constant,stays
R3666	T6188	T6168	punct	.,appear
R3667	T6119	T6120	nmod	knock,out
R3668	T6190	T6191	nmod	Ca2+,proteins
R3669	T6120	T6121	nmod	out,mouse
R367	T1190	T1172	punct	.,led
R3670	T6191	T6195	nsubjpass	proteins,known
R3671	T6192	T6190	cc	and,Ca2+
R3672	T6193	T6194	npadvmod	membrane,skeletal
R3673	T6121	T6118	nmod	mouse,cells
R3674	T6194	T6190	conj	skeletal,Ca2+
R3675	T6122	T6118	amod	red,cells
R3676	T6196	T6195	auxpass	are,known
R3677	T6197	T6198	aux	to,play
R3678	T6198	T6195	xcomp	play,known
R3679	T6123	T6118	compound	blood,cells
R368	T1192	T1193	det	The,protein
R3680	T6199	T6200	amod	key,roles
R3681	T6200	T6198	dobj	roles,play
R3682	T6125	T6126	det	a,shape
R3683	T6201	T6198	prep	on,play
R3684	T6202	T6203	det	the,shape
R3685	T6203	T6201	pobj	shape,on
R3686	T6204	T6205	amod	red,cell
R3687	T6205	T6203	compound	cell,shape
R3688	T6206	T6205	compound	blood,cell
R3689	T6126	T6124	dobj	shape,show
R369	T1193	T1194	nsubj	protein,belongs
R3690	T6127	T6128	advmod	more,flat
R3691	T6207	T6203	compound	membrane,shape
R3692	T6208	T6203	cc	and,shape
R3693	T6209	T6203	conj	stability,shape
R3694	T6128	T6126	amod	flat,shape
R3695	T6210	T6211	punct	[,47
R3696	T6211	T6195	parataxis	47,known
R3697	T6129	T6124	cc	and,show
R3698	T6212	T6211	punct	],47
R3699	T6213	T6195	punct	.,known
R37	T249	T247	compound	kDa,isoform
R370	T1195	T1194	prep	to,belongs
R3700	T6215	T6216	nsubjpass	Annexins,reported
R3701	T6130	T6124	conj	have,show
R3702	T6216	T6219	ccomp	reported,exclude
R3703	T6217	T6216	aux	have,reported
R3704	T6131	T6132	det	a,resistance
R3705	T6218	T6216	auxpass	been,reported
R3706	T6220	T6221	aux	to,bind
R3707	T6239	T6240	advmod	Nevertheless,interact
R3708	T6221	T6216	xcomp	bind,reported
R3709	T6222	T6221	advmod	directly,bind
R371	T1196	T1197	det	a,family
R3710	T6223	T6221	prep	to,bind
R3711	T6224	T6225	compound	F,actin
R3712	T6225	T6223	pobj	actin,to
R3713	T6226	T6225	punct	-,actin
R3714	T6241	T6240	nsubj	it,interact
R3715	T6227	T6219	punct	", ",exclude
R3716	T6228	T6219	nsubj	we,exclude
R3717	T6229	T6219	aux	could,exclude
R3718	T6230	T6219	advmod	however,exclude
R3719	T6242	T6240	aux	might,interact
R372	T1197	T1195	pobj	family,to
R3720	T6231	T6232	amod	such,activity
R3721	T6232	T6219	dobj	activity,exclude
R3722	T6243	T6240	prep	with,interact
R3723	T6233	T6232	det	an,activity
R3724	T6234	T6219	prep	for,exclude
R3725	T6244	T6245	amod	other,components
R3726	T6235	T6236	compound	annexin,A7
R3727	T6236	T6234	pobj	A7,for
R3728	T6237	T6219	punct	.,exclude
R3729	T6245	T6243	pobj	components,with
R373	T1198	T1197	prep	of,family
R3730	T6246	T6240	cc	and,interact
R3731	T6247	T6240	conj	have,interact
R3732	T6248	T6249	det	a,role
R3733	T6249	T6247	dobj	role,have
R3734	T6344	T6345	amod	cytoskeletal,protein
R3735	T6250	T6247	prep	in,have
R3736	T6345	T6346	compound	protein,interactions
R3737	T6346	T6343	pobj	interactions,of
R3738	T6347	T6348	punct	[,50
R3739	T6348	T6330	parataxis	50,shown
R374	T1199	T1200	advmod	evolutionarily,conserved
R3740	T6349	T6348	punct	],50
R3741	T6350	T6330	punct	.,shown
R3742	T6251	T6250	pcomp	organizing,in
R3743	T6352	T6353	nsubj	Ca2+,induces
R3744	T6252	T6253	det	the,cytoskeleton
R3745	T6354	T6353	advmod	also,induces
R3746	T6355	T6356	det	a,redistribution
R3747	T6356	T6353	dobj	redistribution,induces
R3748	T6253	T6251	dobj	cytoskeleton,organizing
R3749	T6357	T6356	compound	transbilayer,redistribution
R375	T1200	T1201	amod	conserved,proteins
R3750	T6358	T6356	compound	phospholipid,redistribution
R3751	T6254	T6253	compound	membrane,cytoskeleton
R3752	T6359	T6356	acl	inducing,redistribution
R3753	T6360	T6361	det	a,change
R3754	T6361	T6359	dobj	change,inducing
R3755	T6255	T6240	punct	", ",interact
R3756	T6362	T6361	compound	shape,change
R3757	T6363	T6361	prep	of,change
R3758	T6256	T6240	advcl	controlling,interact
R3759	T6364	T6365	amod	red,cells
R376	T1201	T1198	pobj	proteins,of
R3760	T6365	T6363	pobj	cells,of
R3761	T6366	T6365	compound	blood,cells
R3762	T6257	T6258	compound	raft,protein
R3763	T6367	T6368	punct	[,51
R3764	T6368	T6353	parataxis	51,induces
R3765	T6369	T6368	punct	],51
R3766	T6258	T6259	compound	protein,associations
R3767	T6370	T6353	punct	.,induces
R3768	T6372	T6373	prep	In,drive
R3769	T6259	T6256	dobj	associations,controlling
R377	T1202	T1201	prep	of,proteins
R3770	T6374	T6372	pobj	addition,In
R3771	T6260	T6256	cc	or,controlling
R3772	T6375	T6373	punct	", ",drive
R3773	T6376	T6377	compound	Ca2+,ions
R3774	T6377	T6373	nsubj	ions,drive
R3775	T6261	T6256	conj	influencing,controlling
R3776	T6378	T6373	aux	can,drive
R3777	T6379	T6373	dobj	spiculation,drive
R3778	T6380	T6379	prep	of,spiculation
R3779	T6381	T6382	det	the,bilayer
R378	T1203	T1204	det	a,structure
R3780	T6262	T6263	det	the,strength
R3781	T6382	T6380	pobj	bilayer,of
R3782	T6383	T6382	compound	membrane,bilayer
R3783	T6263	T6261	dobj	strength,influencing
R3784	T6384	T6373	prep	without,drive
R3785	T6385	T6384	pcomp	involving,without
R3786	T6386	T6387	det	the,cytoskeleton
R3787	T6264	T6263	amod	ionic,strength
R3788	T6387	T6385	dobj	cytoskeleton,involving
R3789	T6388	T6389	punct	[,52
R379	T1204	T1202	pobj	structure,of
R3790	T6389	T6373	parataxis	52,drive
R3791	T6390	T6389	punct	],52
R3792	T6391	T6373	punct	.,drive
R3793	T6265	T6263	prep	of,strength
R3794	T6393	T6394	nsubj	We,observed
R3795	T6266	T6267	amod	red,cells
R3796	T6395	T6394	aux	have,observed
R3797	T6396	T6394	advmod	also,observed
R3798	T6397	T6398	det	a,defect
R3799	T6267	T6265	pobj	cells,of
R38	T250	T248	cc	and,47
R380	T1205	T1204	amod	bipartite,structure
R3800	T6268	T6267	compound	blood,cells
R3801	T6398	T6394	dobj	defect,observed
R3802	T6269	T6256	prep	on,controlling
R3803	T6399	T6398	prep	in,defect
R3804	T6270	T6271	poss	its,own
R3805	T6400	T6401	compound	platelet,function
R3806	T6401	T6399	pobj	function,in
R3807	T6402	T6394	prep	in,observed
R3808	T6403	T6404	det	the,mutant
R3809	T6271	T6269	pobj	own,on
R381	T1206	T1201	prep	with,proteins
R3810	T6404	T6402	pobj	mutant,in
R3811	T6405	T6406	compound	annexin,A7
R3812	T6406	T6404	compound	A7,mutant
R3813	T6272	T6269	cc	or,on
R3814	T6407	T6394	punct	.,observed
R3815	T6273	T6269	conj	by,on
R3816	T6409	T6410	prep	In,was
R3817	T6411	T6412	det	the,experiments
R3818	T6274	T6273	pcomp	interfering,by
R3819	T6412	T6409	pobj	experiments,In
R382	T1207	T1208	det	a,variable
R3820	T6413	T6414	compound	platelet,aggregation
R3821	T6414	T6412	compound	aggregation,experiments
R3822	T6275	T6274	prep	with,interfering
R3823	T6415	T6416	det	the,velocity
R3824	T6416	T6410	nsubj	velocity,was
R3825	T6417	T6416	amod	initial,velocity
R3826	T6276	T6277	amod	other,pathways
R3827	T6418	T6416	compound	aggregation,velocity
R3828	T6419	T6420	advmod	slightly,lower
R3829	T6277	T6275	pobj	pathways,with
R383	T1208	T1206	pobj	variable,with
R3830	T6420	T6410	acomp	lower,was
R3831	T6421	T6419	punct	", ",slightly
R3832	T6422	T6419	cc	but,slightly
R3833	T6423	T6419	conj	significantly,slightly
R3834	T6278	T6277	compound	signaling,pathways
R3835	T6424	T6410	prep	in,was
R3836	T6425	T6424	pobj	platelets,in
R3837	T6279	T6240	punct	.,interact
R3838	T6426	T6425	acl	lacking,platelets
R3839	T6427	T6428	compound	annexin,A7
R384	T1209	T1210	npadvmod	N,terminal
R3840	T6428	T6426	dobj	A7,lacking
R3841	T6281	T6282	mark	As,discussed
R3842	T6429	T6410	punct	.,was
R3843	T6431	T6432	det	These,data
R3844	T6432	T6433	nsubjpass	data,reflected
R3845	T6434	T6433	auxpass	are,reflected
R3846	T6435	T6433	neg	not,reflected
R3847	T6282	T6283	advcl	discussed,involved
R3848	T6436	T6433	agent	by,reflected
R3849	T6437	T6438	det	a,change
R385	T1210	T1208	amod	terminal,variable
R3850	T6438	T6436	pobj	change,by
R3851	T6284	T6282	advmod	above,discussed
R3852	T6439	T6438	prep	in,change
R3853	T6440	T6441	det	the,time
R3854	T6441	T6439	pobj	time,in
R3855	T6442	T6443	advmod	in,vivo
R3856	T6285	T6286	compound	annexin,A7
R3857	T6443	T6441	amod	vivo,time
R3858	T6444	T6441	compound	bleeding,time
R3859	T6445	T6433	punct	.,reflected
R386	T1211	T1210	punct	-,terminal
R3860	T6286	T6283	nsubjpass	A7,involved
R3861	T6447	T6448	advmod	However,is
R3862	T6449	T6450	det	the,speed
R3863	T6287	T6283	aux	could,involved
R3864	T6288	T6283	auxpass	be,involved
R3865	T6289	T6283	prep	in,involved
R3866	T6290	T6291	det	the,homeostasis
R3867	T6291	T6289	pobj	homeostasis,in
R3868	T6292	T6293	amod	cytoplasmic,Ca2+
R3869	T6450	T6448	nsubj	speed,is
R387	T1212	T1208	cc	and,variable
R3870	T6293	T6291	compound	Ca2+,homeostasis
R3871	T6451	T6450	compound	reaction,speed
R3872	T6452	T6450	prep	of,speed
R3873	T6294	T6283	punct	.,involved
R3874	T6453	T6454	amod	murine,platelets
R3875	T6454	T6452	pobj	platelets,of
R3876	T6296	T6297	nsubjpass	It,demonstrated
R3877	T6455	T6456	advmod	much,higher
R3878	T6456	T6448	acomp	higher,is
R3879	T6457	T6456	prep	than,higher
R388	T1213	T1214	det	a,conserved
R3880	T6458	T6457	pobj	those,than
R3881	T6298	T6297	aux	has,demonstrated
R3882	T6459	T6458	prep	of,those
R3883	T6460	T6459	pobj	human,of
R3884	T6461	T6448	cc	and,is
R3885	T6462	T6463	prep	due,missed
R3886	T6299	T6297	auxpass	been,demonstrated
R3887	T6463	T6448	conj	missed,is
R3888	T6464	T6462	pcomp	to,due
R3889	T6300	T6301	mark	that,exert
R389	T1214	T1208	conj	conserved,variable
R3890	T6465	T6466	det	the,setup
R3891	T6466	T6462	pobj	setup,due
R3892	T6467	T6466	amod	technical,setup
R3893	T6301	T6297	ccomp	exert,demonstrated
R3894	T6468	T6463	nsubj	we,missed
R3895	T6469	T6463	aux	might,missed
R3896	T6470	T6463	aux	have,missed
R3897	T6471	T6472	amod	larger,changes
R3898	T6472	T6463	dobj	changes,missed
R3899	T6473	T6463	prep	in,missed
R39	T251	T248	conj	51,47
R390	T1215	T1216	npadvmod	C,terminal
R3900	T6302	T6303	amod	micromolar,changes
R3901	T6474	T6475	det	the,samples
R3902	T6475	T6473	pobj	samples,in
R3903	T6476	T6475	compound	mouse,samples
R3904	T6303	T6301	nsubj	changes,exert
R3905	T6477	T6463	punct	.,missed
R3906	T6304	T6303	prep	of,changes
R3907	T6479	T6480	det	The,reaction
R3908	T6480	T6482	nsubjpass	reaction,caused
R3909	T6481	T6480	amod	immediate,reaction
R391	T1216	T1214	amod	terminal,conserved
R3910	T6305	T6306	det	the,concentration
R3911	T6483	T6480	prep	of,reaction
R3912	T6306	T6304	pobj	concentration,of
R3913	T6484	T6485	compound	mouse,platelets
R3914	T6485	T6483	pobj	platelets,of
R3915	T6307	T6306	amod	intracellular,concentration
R3916	T6486	T6480	cc	and,reaction
R3917	T6308	T6306	compound	Ca2+,concentration
R3918	T6487	T6488	det	the,absence
R3919	T6309	T6310	det	a,effect
R392	T1217	T1216	punct	-,terminal
R3920	T6488	T6480	conj	absence,reaction
R3921	T6489	T6488	prep	of,absence
R3922	T6310	T6301	dobj	effect,exert
R3923	T6490	T6491	det	a,phase
R3924	T6491	T6489	pobj	phase,of
R3925	T6492	T6491	compound	lag,phase
R3926	T6493	T6491	prep	after,phase
R3927	T6311	T6310	amod	profound,effect
R3928	T6494	T6493	pobj	triggering,after
R3929	T6495	T6482	aux	may,caused
R393	T1218	T1208	appos	domain,variable
R3930	T6312	T6310	prep	on,effect
R3931	T6496	T6482	auxpass	be,caused
R3932	T6497	T6482	agent	by,caused
R3933	T6313	T6314	det	the,properties
R3934	T6498	T6499	det	an,generation
R3935	T6499	T6497	pobj	generation,by
R3936	T6500	T6501	advmod	immediately,initiated
R3937	T6314	T6312	pobj	properties,on
R3938	T6501	T6499	amod	initiated,generation
R3939	T6502	T6499	compound	thrombin,generation
R394	T1219	T1194	punct	.,belongs
R3940	T6315	T6314	compound	membrane,properties
R3941	T6503	T6504	punct	[,22
R3942	T6504	T6482	parataxis	22,caused
R3943	T6505	T6504	punct	],22
R3944	T6316	T6317	dep	that,regulate
R3945	T6506	T6482	punct	.,caused
R3946	T6508	T6509	mark	As,known
R3947	T6509	T6510	advcl	known,are
R3948	T6511	T6509	prep	from,known
R3949	T6317	T6314	relcl	regulate,properties
R395	T1221	T1222	det	The,domain
R3950	T6512	T6513	det	the,syndrome
R3951	T6513	T6511	pobj	syndrome,from
R3952	T6514	T6513	amod	frequent,syndrome
R3953	T6318	T6319	amod	red,cell
R3954	T6515	T6513	amod	human,syndrome
R3955	T6516	T6513	compound	vWF,syndrome
R3956	T6517	T6513	punct	-,syndrome
R3957	T6319	T6321	compound	cell,deformability
R3958	T6518	T6513	cc	or,syndrome
R3959	T6519	T6520	amod	other,diseases
R396	T1222	T1226	nsubj	domain,is
R3960	T6320	T6319	compound	blood,cell
R3961	T6520	T6513	conj	diseases,syndrome
R3962	T6521	T6520	amod	haemostatic,diseases
R3963	T6522	T6510	punct	", ",are
R3964	T6321	T6317	dobj	deformability,regulate
R3965	T6523	T6524	amod	small,defects
R3966	T6524	T6510	nsubj	defects,are
R3967	T6322	T6323	punct	[,49
R3968	T6525	T6510	advmod	often,are
R3969	T6526	T6527	advmod	clinically,silent
R397	T1223	T1224	npadvmod	C,terminal
R3970	T6527	T6510	acomp	silent,are
R3971	T6323	T6297	parataxis	49,demonstrated
R3972	T6528	T6510	cc	and,are
R3973	T6529	T6530	aux	do,result
R3974	T6324	T6323	nummod	48,49
R3975	T6530	T6510	conj	result,are
R3976	T6531	T6530	neg	not,result
R3977	T6532	T6530	prep	in,result
R3978	T6533	T6534	amod	physiological,changes
R3979	T6325	T6323	punct	",",49
R398	T1224	T1222	amod	terminal,domain
R3980	T6534	T6532	pobj	changes,in
R3981	T6535	T6510	prep	under,are
R3982	T6326	T6323	punct	],49
R3983	T6536	T6537	amod	normal,conditions
R3984	T6537	T6535	pobj	conditions,under
R3985	T6538	T6510	punct	.,are
R3986	T6327	T6297	punct	.,demonstrated
R3987	T6540	T6541	advmod	Only,in
R3988	T6541	T6542	prep	in,become
R3989	T6329	T6330	advmod	Furthermore,shown
R399	T1225	T1224	punct	-,terminal
R3990	T6543	T6541	pobj	situations,in
R3991	T6544	T6543	prep	with,situations
R3992	T6545	T6546	amod	severe,injury
R3993	T6331	T6330	punct	", ",shown
R3994	T6546	T6544	pobj	injury,with
R3995	T6547	T6546	amod	physical,injury
R3996	T6548	T6542	punct	", ",become
R3997	T6549	T6550	amod	distinct,influences
R3998	T6550	T6542	nsubj	influences,become
R3999	T6551	T6550	amod	environmental,influences
R4	T211	T209	conj	localization,expression
R40	T252	T247	punct	", ",isoform
R400	T1226	T1227	ccomp	is,appears
R4000	T6552	T6550	cc	or,influences
R4001	T6332	T6333	det	the,Ca2+
R4002	T6553	T6554	amod	additional,platelet
R4003	T6554	T6550	conj	platelet,influences
R4004	T6555	T6554	nmod	attributes,platelet
R4005	T6333	T6330	nsubjpass	Ca2+,shown
R4006	T6334	T6333	amod	intracellular,Ca2+
R4007	T6335	T6330	auxpass	was,shown
R4008	T6556	T6554	prep	like,platelet
R4009	T6557	T6558	det	the,differences
R401	T1228	T1226	acomp	responsible,is
R4010	T6336	T6337	aux	to,regulate
R4011	T6558	T6556	pobj	differences,like
R4012	T6559	T6560	nmod	platelet,antigen
R4013	T6337	T6330	xcomp	regulate,shown
R4014	T6560	T6558	nmod	antigen,differences
R4015	T6561	T6558	nmod	polymorphism,differences
R4016	T6562	T6563	punct	[,53
R4017	T6338	T6339	det	the,stability
R4018	T6563	T6558	parataxis	53,differences
R4019	T6564	T6563	punct	],53
R402	T1229	T1228	prep	for,responsible
R4020	T6565	T6542	aux	might,become
R4021	T6339	T6337	dobj	stability,regulate
R4022	T6566	T6542	acomp	apparent,become
R4023	T6567	T6542	punct	.,become
R4024	T6340	T6339	compound	membrane,stability
R4025	T6341	T6337	prep	through,regulate
R4026	T6342	T6341	pobj	modulation,through
R4027	T6343	T6342	prep	of,modulation
R4028	T6643	T6644	prep	In,report
R4029	T6645	T6646	det	this,paper
R403	T1230	T1231	det	the,Ca2+
R4030	T6646	T6643	pobj	paper,In
R4031	T6647	T6644	nsubj	we,report
R4032	T6648	T6649	det	the,presence
R4033	T6649	T6644	dobj	presence,report
R4034	T6650	T6649	prep	of,presence
R4035	T6651	T6652	preconj	both,isoforms
R4036	T6652	T6650	pobj	isoforms,of
R4037	T6653	T6654	compound	annexin,A7
R4038	T6654	T6652	compound	A7,isoforms
R4039	T6655	T6649	prep	in,presence
R404	T1231	T1229	pobj	Ca2+,for
R4040	T6656	T6657	amod	red,cells
R4041	T6657	T6655	pobj	cells,in
R4042	T6658	T6657	compound	blood,cells
R4043	T6659	T6660	advmod	where,are
R4044	T6660	T6657	relcl	are,cells
R4045	T6661	T6660	nsubj	they,are
R4046	T6662	T6660	acomp	abundant,are
R4047	T6663	T6660	prep	in,are
R4048	T6664	T6663	pobj	nanovesicles,in
R4049	T6665	T6666	dep	that,form
R405	T1232	T1231	punct	-,Ca2+
R4050	T6666	T6664	relcl	form,nanovesicles
R4051	T6667	T6666	prep	upon,form
R4052	T6668	T6669	compound	Ca2+,addition
R4053	T6669	T6667	pobj	addition,upon
R4054	T6670	T6644	punct	.,report
R4055	T6672	T6673	det	The,proteins
R4056	T6673	T6674	nsubjpass	proteins,required
R4057	T6675	T6674	auxpass	are,required
R4058	T6676	T6674	advmod	however,required
R4059	T6677	T6674	neg	not,required
R406	T1233	T1231	cc	and,Ca2+
R4060	T6678	T6674	prep	for,required
R4061	T6679	T6680	det	the,process
R4062	T6680	T6678	pobj	process,for
R4063	T6681	T6680	compound	vesiculation,process
R4064	T6682	T6674	punct	.,required
R4065	T6684	T6685	nsubj	They,are
R4066	T6686	T6685	advmod	also,are
R4067	T6687	T6685	neg	not,are
R4068	T6688	T6685	acomp	essential,are
R4069	T6689	T6688	prep	for,essential
R407	T1234	T1231	conj	phospholipid,Ca2+
R4070	T6690	T6691	det	the,formation
R4071	T6691	T6689	pobj	formation,for
R4072	T6692	T6691	prep	of,formation
R4073	T6693	T6694	amod	specific,domains
R4074	T6694	T6692	pobj	domains,of
R4075	T6695	T6694	compound	membrane,domains
R4076	T6696	T6697	amod	such,as
R4077	T6697	T6694	prep	as,domains
R4078	T6698	T6699	compound	lipid,rafts
R4079	T6699	T6697	pobj	rafts,as
R408	T1235	T1231	punct	-,Ca2+
R4080	T6700	T6685	punct	", ",are
R4081	T6701	T6685	prep	as,are
R4082	T6702	T6703	advmod	exogenously,added
R4083	T6703	T6704	amod	added,A7
R4084	T6704	T6701	pobj	A7,as
R4085	T6705	T6704	amod	recombinant,A7
R4086	T6706	T6704	compound	annexin,A7
R4087	T6707	T6704	acl	redistributed,A7
R4088	T6708	T6707	prep	to,redistributed
R4089	T6709	T6710	amod	similar,positions
R409	T1236	T1231	amod	binding,Ca2+
R4090	T6710	T6708	pobj	positions,to
R4091	T6711	T6704	prep	in,A7
R4092	T6712	T6713	det	a,gradient
R4093	T6713	T6711	pobj	gradient,in
R4094	T6714	T6713	compound	density,gradient
R4095	T6715	T6713	acl	containing,gradient
R4096	T6716	T6715	dobj	membranes,containing
R4097	T6717	T6716	acl	derived,membranes
R4098	T6718	T6717	prep	from,derived
R4099	T6719	T6720	amod	red,cells
R41	T253	T254	dep	which,thought
R410	T1237	T1227	punct	", ",appears
R4100	T6720	T6718	pobj	cells,from
R4101	T6721	T6720	compound	blood,cells
R4102	T6722	T6720	prep	of,cells
R4103	T6723	T6724	nmod	anxA7,mice
R4104	T6724	T6722	pobj	mice,of
R4105	T6725	T6723	punct	-,anxA7
R4106	T6726	T6723	punct	/,anxA7
R4107	T6727	T6723	punct	-,anxA7
R4108	T6728	T6685	punct	.,are
R4109	T6730	T6731	det	The,change
R411	T1238	T1239	det	the,domain
R4110	T6731	T6735	nsubj	change,be
R4111	T6732	T6731	amod	observed,change
R4112	T6733	T6734	compound	cell,shape
R4113	T6734	T6731	compound	shape,change
R4114	T6736	T6731	prep	of,change
R4115	T6737	T6736	pobj	erythrocytes,of
R4116	T6738	T6737	acl	lacking,erythrocytes
R4117	T6739	T6740	compound	annexin,A7
R4118	T6740	T6738	dobj	A7,lacking
R4119	T6741	T6735	aux	might,be
R412	T1239	T1227	nsubj	domain,appears
R4120	T6742	T6735	prep	due,be
R4121	T6743	T6742	pcomp	to,due
R4122	T6744	T6742	pobj	alterations,due
R4123	T6745	T6744	prep	in,alterations
R4124	T6746	T6747	det	the,cytoskeleton
R4125	T6747	T6745	pobj	cytoskeleton,in
R4126	T6748	T6747	compound	erythrocyte,cytoskeleton
R4127	T6749	T6735	punct	.,be
R4128	T6751	T6752	mark	As,ruled
R4129	T6752	T6761	advcl	ruled,mediated
R413	T1240	T1241	npadvmod	N,terminal
R4130	T6753	T6754	det	a,interaction
R4131	T6754	T6752	nsubjpass	interaction,ruled
R4132	T6755	T6754	amod	direct,interaction
R4133	T6756	T6754	prep	with,interaction
R4134	T6757	T6758	compound	F,actin
R4135	T6758	T6756	pobj	actin,with
R4136	T6759	T6758	punct	-,actin
R4137	T6760	T6752	auxpass	was,ruled
R4138	T6762	T6752	prt	out,ruled
R4139	T6763	T6761	punct	", ",mediated
R414	T1241	T1239	amod	terminal,domain
R4140	T6764	T6765	det	this,effect
R4141	T6765	T6761	nsubjpass	effect,mediated
R4142	T6766	T6761	aux	might,mediated
R4143	T6767	T6761	auxpass	be,mediated
R4144	T6768	T6761	agent	by,mediated
R4145	T6769	T6770	poss	annexin,activity
R4146	T6770	T6768	pobj	activity,by
R4147	T6771	T6769	case	's,annexin
R4148	T6772	T6773	npadvmod	Ca2+,binding
R4149	T6773	T6770	amod	binding,activity
R415	T1242	T1241	punct	-,terminal
R4150	T6774	T6773	punct	-,binding
R4151	T6775	T6761	punct	.,mediated
R4152	T6880	T6879	prep	of,Acquisition
R4153	T6881	T6882	nmod	murine,blood
R4154	T6882	T6885	compound	blood,samples
R4155	T6883	T6881	cc	and,murine
R4156	T6884	T6881	conj	human,murine
R4157	T6885	T6880	pobj	samples,of
R4158	T6887	T6888	nsubjpass	Blood,collected
R4159	T6889	T6887	prep	from,Blood
R416	T1243	T1244	aux	to,confer
R4160	T6890	T6891	nmod	129Sv,mice
R4161	T6891	T6889	pobj	mice,from
R4162	T6892	T6893	amod	wild,type
R4163	T6893	T6891	nmod	type,mice
R4164	T6894	T6893	cc	and,type
R4165	T6895	T6896	compound	annexin,A7
R4166	T6896	T6893	conj	A7,type
R4167	T6897	T6898	compound	knock,out
R4168	T6898	T6891	compound	out,mice
R4169	T6899	T6900	punct	(,anxA7
R417	T1244	T1227	xcomp	confer,appears
R4170	T6900	T6891	parataxis	anxA7,mice
R4171	T6901	T6900	punct	-,anxA7
R4172	T6902	T6900	punct	/,anxA7
R4173	T6903	T6900	punct	-,anxA7
R4174	T6904	T6900	punct	),anxA7
R4175	T6905	T6887	acl	kept,Blood
R4176	T6906	T6905	prep	under,kept
R4177	T6907	T6908	npadvmod	pathogen,free
R4178	T6908	T6910	amod	free,conditions
R4179	T6909	T6908	punct	-,free
R418	T1245	T1246	amod	functional,diversity
R4180	T6910	T6906	pobj	conditions,under
R4181	T6911	T6905	cc	and,kept
R4182	T6912	T6905	conj	fed,kept
R4183	T6913	T6912	prep	with,fed
R4184	T6914	T6915	amod	regular,food
R4185	T6915	T6913	pobj	food,with
R4186	T6916	T6888	punct	", ",collected
R4187	T6917	T6888	auxpass	was,collected
R4188	T6918	T6888	prep	in,collected
R4189	T6919	T6920	nummod	1,ml
R419	T1246	T1244	dobj	diversity,confer
R4190	T6920	T6921	compound	ml,syringes
R4191	T6921	T6918	pobj	syringes,in
R4192	T6922	T6888	prep	with,collected
R4193	T6923	T6924	det	a,needle
R4194	T6924	T6927	nsubj	needle,punctating
R4195	T6925	T6926	nummod	22,gauge
R4196	T6926	T6924	compound	gauge,needle
R4197	T6927	T6922	pcomp	punctating,with
R4198	T6928	T6929	det	the,ventricle
R4199	T6929	T6927	dobj	ventricle,punctating
R42	T254	T247	relcl	thought,isoform
R420	T1247	T1248	punct	[,2
R4200	T6930	T6929	amod	right,ventricle
R4201	T6931	T6929	amod	cardiac,ventricle
R4202	T6932	T6933	advmod	immediately,killed
R4203	T6933	T6927	advcl	killed,punctating
R4204	T6934	T6933	mark	after,killed
R4205	T6935	T6933	nsubjpass	they,killed
R4206	T6936	T6933	aux	had,killed
R4207	T6937	T6933	auxpass	been,killed
R4208	T6938	T6933	prep	by,killed
R4209	T6939	T6940	amod	cervical,dislocation
R421	T1248	T1227	parataxis	2,appears
R4210	T6940	T6938	pobj	dislocation,by
R4211	T6941	T6888	punct	.,collected
R4212	T6943	T6944	nsubjpass	Coagulation,inhibited
R4213	T6945	T6944	advmod	generally,inhibited
R4214	T6946	T6944	auxpass	was,inhibited
R4215	T6947	T6944	prep	with,inhibited
R4216	T6948	T6949	quantmod	1,10
R4217	T6949	T6951	nummod	10,volume
R4218	T6950	T6949	punct	/,10
R4219	T6951	T6947	pobj	volume,with
R422	T1249	T1250	punct	-,4
R4220	T6952	T6951	prep	of,volume
R4221	T6953	T6954	nummod	10,mM
R4222	T6954	T6955	compound	mM,NaEDTA
R4223	T6955	T6952	pobj	NaEDTA,of
R4224	T6956	T6955	punct	", ",NaEDTA
R4225	T6957	T6955	npadvmod	pH,NaEDTA
R4226	T6958	T6957	nummod	7.5,pH
R4227	T6959	T6951	punct	", ",volume
R4228	T6960	T6961	advmod	already,present
R4229	T6961	T6951	relcl	present,volume
R423	T1250	T1248	prep	4,2
R4230	T6962	T6961	prep	in,present
R4231	T6963	T6964	det	the,syringe
R4232	T6964	T6962	pobj	syringe,in
R4233	T6965	T6944	punct	.,inhibited
R4234	T6967	T6968	det	The,mice
R4235	T6968	T6969	nsubjpass	mice,killed
R4236	T6970	T6969	auxpass	were,killed
R4237	T6971	T6969	prep	by,killed
R4238	T6972	T6973	amod	cervical,dislocation
R4239	T6973	T6971	pobj	dislocation,by
R424	T1251	T1248	punct	],2
R4240	T6974	T6969	prep	in,killed
R4241	T6975	T6974	pobj	order,in
R4242	T6976	T6977	aux	to,avoid
R4243	T6977	T6975	acl	avoid,order
R4244	T6978	T6979	det	any,effect
R4245	T6979	T6977	dobj	effect,avoid
R4246	T6980	T6979	prep	of,effect
R4247	T6981	T6982	amod	anaesthetic,chemicals
R4248	T6982	T6980	pobj	chemicals,of
R4249	T6983	T6979	prep	on,effect
R425	T1252	T1227	punct	.,appears
R4250	T6984	T6985	compound	blood,parameters
R4251	T6985	T6983	pobj	parameters,on
R4252	T6986	T6969	punct	.,killed
R4253	T6988	T6989	det	The,mice
R4254	T6989	T6994	nsubjpass	mice,matched
R4255	T6990	T6991	amod	wild,type
R4256	T6991	T6989	nmod	type,mice
R4257	T6992	T6991	cc	and,type
R4258	T6993	T6991	conj	mutant,type
R4259	T6995	T6994	auxpass	were,matched
R426	T1254	T1255	det	The,binding
R4260	T6996	T6994	prep	regarding,matched
R4261	T6997	T6998	poss	their,age
R4262	T6998	T6996	pobj	age,regarding
R4263	T6999	T6998	punct	", ",age
R4264	T7000	T6998	conj	weight,age
R4265	T7001	T7000	cc	and,weight
R4266	T7002	T7000	conj	sex,weight
R4267	T7003	T6994	punct	.,matched
R4268	T7005	T7006	det	The,daytime
R4269	T7006	T7007	nsubjpass	daytime,kept
R427	T1255	T1256	nsubjpass	binding,thought
R4270	T7008	T7006	prep	of,daytime
R4271	T7009	T7010	compound	blood,collection
R4272	T7010	T7008	pobj	collection,of
R4273	T7011	T7006	punct	", ",daytime
R4274	T7012	T7013	amod	sampling,site
R4275	T7013	T7006	conj	site,daytime
R4276	T7014	T7013	cc	and,site
R4277	T7015	T7016	compound	collection,method
R4278	T7016	T7013	conj	method,site
R4279	T7017	T7007	auxpass	were,kept
R428	T1257	T1255	prep	to,binding
R4280	T7018	T7007	oprd	constant,kept
R4281	T7019	T7007	prep	during,kept
R4282	T7020	T7021	det	all,experiments
R4283	T7021	T7019	pobj	experiments,during
R4284	T7022	T7007	punct	.,kept
R4285	T7024	T7025	det	A,volume
R4286	T7025	T7030	nsubj	volume,consisted
R4287	T7026	T7025	amod	typical,volume
R4288	T7027	T7028	amod	murine,sample
R4289	T7028	T7025	compound	sample,volume
R429	T1258	T1259	advmod	negatively,charged
R4290	T7029	T7028	compound	blood,sample
R4291	T7031	T7030	prep	of,consisted
R4292	T7032	T7033	nummod	550,μl
R4293	T7033	T7031	pobj	μl,of
R4294	T7034	T7030	punct	", ",consisted
R4295	T7035	T7030	prep	with,consisted
R4296	T7036	T7035	pobj	variations,with
R4297	T7037	T7036	acl	ranging,variations
R4298	T7038	T7037	prep	from,ranging
R4299	T7039	T7040	nummod	200,μl
R43	T255	T254	auxpass	is,thought
R430	T1259	T1260	amod	charged,phospholipids
R4300	T7040	T7038	pobj	μl,from
R4301	T7041	T7038	prep	to,from
R4302	T7042	T7043	nummod	900,μl
R4303	T7043	T7041	pobj	μl,to
R4304	T7044	T7030	punct	.,consisted
R4305	T7046	T7047	amod	Human,samples
R4306	T7047	T7049	nsubjpass	samples,collected
R4307	T7048	T7047	compound	blood,samples
R4308	T7050	T7047	prep	from,samples
R4309	T7051	T7052	det	the,authors
R431	T1260	T1257	pobj	phospholipids,to
R4310	T7052	T7050	pobj	authors,from
R4311	T7053	T7049	auxpass	were,collected
R4312	T7054	T7049	prep	by,collected
R4313	T7055	T7056	compound	vein,puncture
R4314	T7056	T7054	pobj	puncture,by
R4315	T7057	T7049	advcl	using,collected
R4316	T7058	T7059	amod	common,monovettes
R4317	T7059	T7057	dobj	monovettes,using
R4318	T7060	T7059	compound	EDTA,monovettes
R4319	T7061	T7059	punct	-,monovettes
R432	T1261	T1256	auxpass	is,thought
R4320	T7062	T7063	punct	(,Sarstedt
R4321	T7063	T7049	parataxis	Sarstedt,collected
R4322	T7064	T7063	punct	),Sarstedt
R4323	T7065	T7049	punct	.,collected
R4324	T7067	T7068	nsubj	We,performed
R4325	T7069	T7070	poss	our,studies
R4326	T7070	T7068	dobj	studies,performed
R4327	T7071	T7068	advcl	using,performed
R4328	T7072	T7073	advmod	well,standardized
R4329	T7073	T7074	amod	standardized,conditions
R433	T1262	T1263	aux	to,mediated
R4330	T7074	T7071	dobj	conditions,using
R4331	T7075	T7074	compound	sampling,conditions
R4332	T7076	T7068	punct	", ",performed
R4333	T7077	T7078	mark	as,show
R4334	T7078	T7068	advcl	show,performed
R4335	T7079	T7078	nsubj	differences,show
R4336	T7080	T7079	prep	in,differences
R4337	T7081	T7082	det	the,site
R4338	T7082	T7080	pobj	site,in
R4339	T7083	T7084	compound	blood,sampling
R434	T1263	T1256	xcomp	mediated,thought
R4340	T7084	T7082	compound	sampling,site
R4341	T7085	T7079	punct	", ",differences
R4342	T7086	T7087	prep	for,between
R4343	T7087	T7079	prep	between,differences
R4344	T7088	T7086	pobj	example,for
R4345	T7089	T7087	pobj	tail,between
R4346	T7090	T7089	cc	and,tail
R4347	T7091	T7089	conj	heart,tail
R4348	T7092	T7078	punct	", ",show
R4349	T7093	T7094	amod	large,changes
R435	T1264	T1263	auxpass	be,mediated
R4350	T7094	T7078	dobj	changes,show
R4351	T7095	T7094	prep	in,changes
R4352	T7096	T7097	det	all,counts
R4353	T7097	T7095	pobj	counts,in
R4354	T7098	T7099	compound	cell,type
R4355	T7099	T7097	compound	type,counts
R4356	T7100	T7068	punct	.,performed
R4357	T7102	T7103	amod	First,draw
R4358	T7103	T7107	nsubj	draw,affect
R4359	T7104	T7102	cc	and,First
R436	T1265	T1263	agent	by,mediated
R4360	T7105	T7102	conj	second,First
R4361	T7106	T7103	compound	sample,draw
R4362	T7108	T7109	compound	blood,measurements
R4363	T7109	T7107	dobj	measurements,affect
R4364	T7110	T7107	punct	", ",affect
R4365	T7111	T7107	advmod	too,affect
R4366	T7112	T7113	punct	[,22
R4367	T7113	T7107	parataxis	22,affect
R4368	T7114	T7113	punct	],22
R4369	T7115	T7107	punct	.,affect
R437	T1266	T1267	compound	Ca2+,ions
R4370	T7117	T7118	advmod	Also,affects
R4371	T7119	T7120	det	the,background
R4372	T7120	T7118	nsubj	background,affects
R4373	T7121	T7120	amod	genetic,background
R4374	T7122	T7120	prep	in,background
R4375	T7123	T7124	amod	common,mice
R4376	T7124	T7122	pobj	mice,in
R4377	T7125	T7124	nmod	laboratory,mice
R4378	T7126	T7125	cc	and,laboratory
R4379	T7127	T7125	conj	transgenic,laboratory
R438	T1267	T1265	pobj	ions,by
R4380	T7128	T7129	det	the,phenotype
R4381	T7129	T7118	dobj	phenotype,affects
R4382	T7130	T7118	punct	.,affects
R4383	T7132	T7133	det	A,change
R4384	T7133	T7134	nsubj	change,protect
R4385	T7135	T7133	prep	from,change
R4386	T7136	T7137	nummod	one,strain
R4387	T7137	T7135	pobj	strain,from
R4388	T7138	T7133	prep	to,change
R4389	T7139	T7138	pobj	another,to
R439	T1268	T1256	punct	.,thought
R4390	T7140	T7134	aux	may,protect
R4391	T7141	T7134	dobj	mice,protect
R4392	T7142	T7134	prep	from,protect
R4393	T7143	T7142	pobj	effects,from
R4394	T7144	T7143	prep	of,effects
R4395	T7145	T7146	det	the,defect
R4396	T7146	T7144	pobj	defect,of
R4397	T7147	T7148	advmod	primarily,induced
R4398	T7148	T7146	amod	induced,defect
R4399	T7149	T7146	amod	genetic,defect
R44	T256	T257	aux	to,involved
R440	T1270	T1271	compound	Annexin,A7
R4400	T7150	T7134	punct	.,protect
R4401	T7152	T7153	prep	For,have
R4402	T7154	T7152	pobj	example,For
R4403	T7155	T7153	punct	", ",have
R4404	T7156	T7157	compound	BALB,c
R4405	T7157	T7159	compound	c,mice
R4406	T7158	T7157	punct	/,c
R4407	T7159	T7153	nsubj	mice,have
R4408	T7160	T7161	det	a,velocity
R4409	T7161	T7153	dobj	velocity,have
R441	T1271	T1272	nsubj	A7,is
R4410	T7162	T7163	advmod	significantly,increased
R4411	T7163	T7161	amod	increased,velocity
R4412	T7164	T7161	prep	of,velocity
R4413	T7165	T7166	compound	platelet,aggregation
R4414	T7166	T7164	pobj	aggregation,of
R4415	T7167	T7153	prep	as,have
R4416	T7168	T7167	prep	compared,as
R4417	T7169	T7168	prep	to,compared
R4418	T7170	T7171	compound	129Sv,mice
R4419	T7171	T7169	pobj	mice,to
R442	T1273	T1272	acomp	unique,is
R4420	T7172	T7171	punct	", ",mice
R4421	T7173	T7174	dep	which,used
R4422	T7174	T7171	relcl	used,mice
R4423	T7175	T7174	auxpass	were,used
R4424	T7176	T7174	prep	throughout,used
R4425	T7177	T7178	det	this,study
R4426	T7178	T7176	pobj	study,throughout
R4427	T7179	T7180	punct	[,23
R4428	T7180	T7153	parataxis	23,have
R4429	T7181	T7180	punct	],23
R443	T1274	T1275	mark	in,carries
R4430	T7182	T7153	punct	.,have
R4431	T7273	T7274	npadvmod	Self,forming
R4432	T7274	T7275	amod	forming,gradients
R4433	T7276	T7275	compound	iodixanol,gradients
R4434	T7277	T7275	compound	density,gradients
R4435	T7278	T7275	prep	of,gradients
R4436	T7279	T7280	amod	human,cells
R4437	T7280	T7278	pobj	cells,of
R4438	T7281	T7280	amod	red,cells
R4439	T7282	T7280	compound	blood,cells
R444	T1275	T1272	advcl	carries,is
R4440	T7284	T7285	det	The,distribution
R4441	T7285	T7287	nsubjpass	distribution,addressed
R4442	T7286	T7285	amod	subcellular,distribution
R4443	T7288	T7285	prep	of,distribution
R4444	T7289	T7290	compound	annexin,A7
R4445	T7290	T7288	pobj	A7,of
R4446	T7291	T7287	auxpass	was,addressed
R4447	T7292	T7287	prep	according,addressed
R4448	T7293	T7292	prep	to,according
R4449	T7294	T7293	punct	[,to
R445	T1276	T1275	mark	that,carries
R4450	T7295	T7293	pobj	24,to
R4451	T7296	T7287	punct	],addressed
R4452	T7297	T7287	punct	.,addressed
R4453	T7299	T7300	amod	Red,cells
R4454	T7300	T7302	nsubjpass	cells,centrifuged
R4455	T7301	T7300	compound	blood,cells
R4456	T7303	T7302	auxpass	were,centrifuged
R4457	T7304	T7302	cc	and,centrifuged
R4458	T7305	T7302	conj	washed,centrifuged
R4459	T7306	T7305	prep	in,washed
R446	T1277	T1275	nsubj	it,carries
R4460	T7307	T7308	amod	isotonic,solution
R4461	T7308	T7306	pobj	solution,in
R4462	T7309	T7310	amod	buffered,salt
R4463	T7310	T7308	compound	salt,solution
R4464	T7311	T7305	punct	", ",washed
R4465	T7312	T7305	conj	collected,washed
R4466	T7313	T7312	prep	in,collected
R4467	T7314	T7315	npadvmod	ice,cold
R4468	T7315	T7316	amod	cold,buffer
R4469	T7316	T7313	pobj	buffer,in
R447	T1278	T1279	det	an,terminus
R4470	T7317	T7316	compound	lysis,buffer
R4471	T7318	T7319	punct	(,Hepes
R4472	T7319	T7316	parataxis	Hepes,buffer
R4473	T7320	T7321	nummod	20,mM
R4474	T7321	T7319	compound	mM,Hepes
R4475	T7322	T7319	punct	", ",Hepes
R4476	T7323	T7319	npadvmod	pH,Hepes
R4477	T7324	T7323	nummod	7.4,pH
R4478	T7325	T7319	punct	", ",Hepes
R4479	T7326	T7327	nummod	200,μM
R448	T1279	T1275	dobj	terminus,carries
R4480	T7327	T7328	compound	μM,CaCl2
R4481	T7328	T7319	conj	CaCl2,Hepes
R4482	T7329	T7328	punct	", ",CaCl2
R4483	T7330	T7331	nummod	0.25,M
R4484	T7331	T7332	compound	M,sucrose
R4485	T7332	T7328	conj	sucrose,CaCl2
R4486	T7333	T7332	punct	", ",sucrose
R4487	T7334	T7332	cc	and,sucrose
R4488	T7335	T7336	compound	proteinase,inhibitors
R4489	T7336	T7332	conj	inhibitors,sucrose
R449	T1280	T1281	advmod	extraordinarily,long
R4490	T7337	T7338	punct	(,Roche
R4491	T7338	T7336	parataxis	Roche,inhibitors
R4492	T7339	T7338	punct	),Roche
R4493	T7340	T7336	punct	),inhibitors
R4494	T7341	T7312	punct	", ",collected
R4495	T7342	T7312	conj	homogenized,collected
R4496	T7343	T7342	prep	by,homogenized
R4497	T7344	T7345	det	a,homogenizer
R4498	T7345	T7343	pobj	homogenizer,by
R4499	T7346	T7347	advmod	loosely,fitting
R45	T257	T254	xcomp	involved,thought
R450	T1281	T1279	amod	long,terminus
R4500	T7347	T7345	amod	fitting,homogenizer
R4501	T7348	T7345	compound	dounce,homogenizer
R4502	T7349	T7345	cc	and,homogenizer
R4503	T7350	T7351	amod	additional,passages
R4504	T7351	T7345	conj	passages,homogenizer
R4505	T7352	T7351	prep	through,passages
R4506	T7353	T7354	det	a,needle
R4507	T7354	T7352	pobj	needle,through
R4508	T7355	T7356	nummod	22,gauge
R4509	T7356	T7354	compound	gauge,needle
R451	T1282	T1281	cc	and,long
R4510	T7357	T7302	punct	.,centrifuged
R4511	T7359	T7360	det	A,solution
R4512	T7360	T7364	nsubjpass	solution,established
R4513	T7361	T7362	nummod	50,%
R4514	T7362	T7360	compound	%,solution
R4515	T7363	T7360	compound	iodixanol,solution
R4516	T7365	T7364	auxpass	was,established
R4517	T7366	T7364	prep	by,established
R4518	T7367	T7366	pcomp	mixing,by
R4519	T7368	T7369	compound	Optiprep,solution
R452	T1283	T1281	conj	hydrophobic,long
R4520	T7369	T7367	dobj	solution,mixing
R4521	T7370	T7371	punct	(,Sigma
R4522	T7371	T7369	parataxis	Sigma,solution
R4523	T7372	T7373	nummod	60,%
R4524	T7373	T7374	compound	%,iodixanol
R4525	T7374	T7371	dep	iodixanol,Sigma
R4526	T7375	T7371	punct	;,Sigma
R4527	T7376	T7371	punct	),Sigma
R4528	T7377	T7367	prep	with,mixing
R4529	T7378	T7377	pobj	buffer,with
R453	T1284	T1279	compound	amino,terminus
R4530	T7379	T7380	punct	(,sucrose
R4531	T7380	T7378	parataxis	sucrose,buffer
R4532	T7381	T7382	nummod	120,mM
R4533	T7382	T7383	compound	mM,Hepes
R4534	T7383	T7380	dep	Hepes,sucrose
R4535	T7384	T7383	punct	", ",Hepes
R4536	T7385	T7383	npadvmod	pH,Hepes
R4537	T7386	T7385	nummod	7.4,pH
R4538	T7387	T7380	punct	", ",sucrose
R4539	T7388	T7389	nummod	1.2,mM
R454	T1285	T1279	prep	with,terminus
R4540	T7389	T7390	compound	mM,CaCl2
R4541	T7390	T7380	dep	CaCl2,sucrose
R4542	T7391	T7380	punct	", ",sucrose
R4543	T7392	T7393	nummod	0.25,M
R4544	T7393	T7380	compound	M,sucrose
R4545	T7394	T7380	punct	),sucrose
R4546	T7395	T7364	punct	.,established
R4547	T7397	T7398	nummod	Seven,parts
R4548	T7398	T7399	nsubjpass	parts,mixed
R4549	T7400	T7398	prep	of,parts
R455	T1286	T1287	amod	more,100
R4550	T7401	T7402	det	this,solution
R4551	T7402	T7400	pobj	solution,of
R4552	T7403	T7404	nummod	50,%
R4553	T7404	T7402	compound	%,solution
R4554	T7405	T7402	compound	iodixanol,solution
R4555	T7406	T7399	auxpass	were,mixed
R4556	T7407	T7399	prep	with,mixed
R4557	T7408	T7409	nummod	thirteen,parts
R4558	T7409	T7407	pobj	parts,with
R4559	T7410	T7409	prep	of,parts
R456	T1287	T1289	nummod	100,acids
R4560	T7411	T7412	det	the,solution
R4561	T7412	T7410	pobj	solution,of
R4562	T7413	T7414	amod	red,cell
R4563	T7414	T7412	compound	cell,solution
R4564	T7415	T7414	compound	blood,cell
R4565	T7416	T7399	punct	", ",mixed
R4566	T7417	T7399	advcl	generating,mixed
R4567	T7418	T7419	det	a,solution
R4568	T7419	T7417	dobj	solution,generating
R4569	T7420	T7419	compound	gradient,solution
R457	T1288	T1287	quantmod	than,100
R4570	T7421	T7419	acl	containing,solution
R4571	T7422	T7423	nummod	17.5,%
R4572	T7423	T7424	compound	%,iodixanol
R4573	T7424	T7421	dobj	iodixanol,containing
R4574	T7425	T7399	punct	.,mixed
R4575	T7427	T7428	det	The,mixture
R4576	T7428	T7429	nsubj	mixture,filled
R4577	T7430	T7429	aux	was,filled
R4578	T7431	T7429	prep	into,filled
R4579	T7432	T7433	det	a,tube
R458	T1289	T1285	pobj	acids,with
R4580	T7433	T7431	pobj	tube,into
R4581	T7434	T7433	amod	centrifuge,tube
R4582	T7435	T7433	amod	underlayed,tube
R4583	T7436	T7435	prep	by,underlayed
R4584	T7437	T7436	pobj	cushions,by
R4585	T7438	T7437	prep	of,cushions
R4586	T7439	T7440	nummod	30,%
R4587	T7440	T7441	nmod	%,iodixanol
R4588	T7441	T7438	pobj	iodixanol,of
R4589	T7442	T7440	cc	and,%
R459	T1290	T1289	compound	amino,acids
R4590	T7443	T7444	nummod	35,%
R4591	T7444	T7440	conj	%,%
R4592	T7445	T7429	cc	and,filled
R4593	T7446	T7429	conj	centrifuged,filled
R4594	T7447	T7446	prep	for,centrifuged
R4595	T7448	T7449	nummod	3,hours
R4596	T7449	T7447	pobj	hours,for
R4597	T7450	T7446	prep	at,centrifuged
R4598	T7451	T7452	nummod	"270,000",g
R4599	T7452	T7450	pobj	g,at
R46	T258	T257	auxpass	be,involved
R460	T1291	T1272	punct	.,is
R4600	T7453	T7452	punct	×,g
R4601	T7454	T7446	prep	in,centrifuged
R4602	T7455	T7456	det	a,rotor
R4603	T7456	T7454	pobj	rotor,in
R4604	T7457	T7458	compound	SW41,Ti
R4605	T7458	T7456	compound	Ti,rotor
R4606	T7459	T7460	compound	swing,out
R4607	T7460	T7456	compound	out,rotor
R4608	T7461	T7462	punct	(,Beckman
R4609	T7462	T7456	parataxis	Beckman,rotor
R461	T1293	T1294	amod	Alternative,splicing
R4610	T7463	T7462	punct	),Beckman
R4611	T7464	T7465	aux	to,achieve
R4612	T7465	T7429	advcl	achieve,filled
R4613	T7466	T7467	det	a,gradient
R4614	T7467	T7465	dobj	gradient,achieve
R4615	T7468	T7469	advmod	nearly,linear
R4616	T7469	T7467	amod	linear,gradient
R4617	T7470	T7467	compound	density,gradient
R4618	T7471	T7472	punct	(,g
R4619	T7472	T7467	parataxis	g,gradient
R462	T1294	T1295	nsubj	splicing,gives
R4620	T7473	T7472	advmod	approximately,g
R4621	T7474	T7472	nummod	1.06,g
R4622	T7475	T7476	punct	/,ml
R4623	T7476	T7472	prep	ml,g
R4624	T7477	T7472	prep	to,g
R4625	T7478	T7479	nummod	1.20,g
R4626	T7479	T7477	pobj	g,to
R4627	T7480	T7481	punct	/,ml
R4628	T7481	T7479	prep	ml,g
R4629	T7482	T7472	punct	),g
R463	T1296	T1295	dobj	rise,gives
R4630	T7483	T7484	punct	[,24
R4631	T7484	T7429	parataxis	24,filled
R4632	T7485	T7484	punct	],24
R4633	T7486	T7429	punct	.,filled
R4634	T7488	T7489	nsubjpass	Fractions,collected
R4635	T7490	T7488	prep	of,Fractions
R4636	T7491	T7492	nummod	0.6,ml
R4637	T7492	T7490	pobj	ml,of
R4638	T7493	T7489	auxpass	were,collected
R4639	T7494	T7489	prep	from,collected
R464	T1297	T1295	prep	to,gives
R4640	T7495	T7496	det	the,top
R4641	T7496	T7494	pobj	top,from
R4642	T7497	T7496	prep	of,top
R4643	T7498	T7499	det	the,gradient
R4644	T7499	T7497	pobj	gradient,of
R4645	T7500	T7489	prep	by,collected
R4646	T7501	T7502	det	a,syringe
R4647	T7502	T7500	pobj	syringe,by
R4648	T7503	T7504	nummod	1,ml
R4649	T7504	T7502	compound	ml,syringe
R465	T1298	T1299	nummod	two,isoforms
R4650	T7505	T7489	prep	without,collected
R4651	T7506	T7505	pcomp	using,without
R4652	T7507	T7508	det	a,needle
R4653	T7508	T7506	dobj	needle,using
R4654	T7509	T7489	cc	and,collected
R4655	T7510	T7511	advmod	immediately,frozen
R4656	T7511	T7489	conj	frozen,collected
R4657	T7512	T7511	prep	in,frozen
R4658	T7513	T7514	amod	liquid,nitrogen
R4659	T7514	T7512	pobj	nitrogen,in
R466	T1299	T1297	pobj	isoforms,to
R4660	T7515	T7511	prep	for,frozen
R4661	T7516	T7517	amod	further,use
R4662	T7517	T7515	pobj	use,for
R4663	T7518	T7489	punct	.,collected
R4664	T7632	T7633	compound	Exovesicle,generation
R4665	T7634	T7633	prep	from,generation
R4666	T7635	T7636	amod	human,cells
R4667	T7636	T7634	pobj	cells,from
R4668	T7637	T7635	cc	and,human
R4669	T7638	T7635	conj	murine,human
R467	T1300	T1299	prep	of,isoforms
R4670	T7639	T7636	amod	red,cells
R4671	T7640	T7636	compound	blood,cells
R4672	T7642	T7643	amod	Red,cells
R4673	T7643	T7645	nsubj	cells,form
R4674	T7644	T7643	compound	blood,cells
R4675	T7646	T7645	advmod	naturally,form
R4676	T7647	T7645	aux	can,form
R4677	T7648	T7649	npadvmod	hemoglobin,containing
R4678	T7649	T7650	amod	containing,exovesicles
R4679	T7650	T7645	dobj	exovesicles,form
R468	T1301	T1302	nummod	47,kDa
R4680	T7651	T7645	punct	.,form
R4681	T7653	T7654	advmod	In,vitro
R4682	T7654	T7655	advmod	vitro,is
R4683	T7656	T7657	det	these,formation
R4684	T7657	T7655	nsubj	formation,is
R4685	T7658	T7657	compound	vesicle,formation
R4686	T7659	T7660	npadvmod	Ca2+,induced
R4687	T7660	T7655	acomp	induced,is
R4688	T7661	T7655	punct	.,is
R4689	T7663	T7664	advmod	Freshly,collected
R469	T1302	T1300	pobj	kDa,of
R4690	T7664	T7665	amod	collected,samples
R4691	T7665	T7671	nsubjpass	samples,centrifuged
R4692	T7666	T7667	nmod	lithium,heparin
R4693	T7667	T7665	nmod	heparin,samples
R4694	T7668	T7667	punct	-,heparin
R4695	T7669	T7665	amod	anticoagulated,samples
R4696	T7670	T7665	compound	blood,samples
R4697	T7672	T7671	auxpass	were,centrifuged
R4698	T7673	T7671	prep	for,centrifuged
R4699	T7674	T7675	nummod	5,minutes
R47	T259	T257	prep	in,involved
R470	T1303	T1301	cc	and,47
R4700	T7675	T7673	pobj	minutes,for
R4701	T7676	T7671	prep	at,centrifuged
R4702	T7677	T7678	nummod	400,g
R4703	T7678	T7676	pobj	g,at
R4704	T7679	T7678	punct	×,g
R4705	T7680	T7678	cc	and,g
R4706	T7681	T7682	nummod	4,°C
R4707	T7682	T7678	conj	°C,g
R4708	T7683	T7671	punct	.,centrifuged
R4709	T7685	T7686	det	The,erythrocytes
R471	T1304	T1301	conj	51,47
R4710	T7686	T7687	nsubjpass	erythrocytes,washed
R4711	T7688	T7687	auxpass	were,washed
R4712	T7689	T7690	nummod	five,times
R4713	T7690	T7687	npadvmod	times,washed
R4714	T7691	T7687	prep	with,washed
R4715	T7692	T7693	nummod	20,mM
R4716	T7693	T7694	compound	mM,Hepes
R4717	T7694	T7691	pobj	Hepes,with
R4718	T7695	T7694	punct	", ",Hepes
R4719	T7696	T7694	npadvmod	pH,Hepes
R472	T1305	T1295	punct	.,gives
R4720	T7697	T7696	nummod	7.5,pH
R4721	T7698	T7694	punct	", ",Hepes
R4722	T7699	T7694	cc	and,Hepes
R4723	T7700	T7701	nummod	150,mM
R4724	T7701	T7702	compound	mM,NaCl
R4725	T7702	T7694	conj	NaCl,Hepes
R4726	T7703	T7704	punct	(,buffer
R4727	T7704	T7702	parataxis	buffer,NaCl
R4728	T7705	T7704	amod	washing,buffer
R4729	T7706	T7704	punct	),buffer
R473	T1307	T1308	det	Both,isoforms
R4730	T7707	T7687	punct	.,washed
R4731	T7709	T7710	det	A,volume
R4732	T7710	T7711	nsubjpass	volume,combined
R4733	T7712	T7710	prep	of,volume
R4734	T7713	T7714	nummod	500,μl
R4735	T7714	T7715	nmod	μl,cells
R4736	T7715	T7712	pobj	cells,of
R4737	T7716	T7715	amod	washed,cells
R4738	T7717	T7715	amod	red,cells
R4739	T7718	T7715	compound	blood,cells
R474	T1308	T1309	nsubj	isoforms,differ
R4740	T7719	T7711	auxpass	were,combined
R4741	T7720	T7711	prep	with,combined
R4742	T7721	T7722	nummod	3,ml
R4743	T7722	T7720	pobj	ml,with
R4744	T7723	T7722	prep	of,ml
R4745	T7724	T7725	compound	vesiculation,buffer
R4746	T7725	T7723	pobj	buffer,of
R4747	T7726	T7727	punct	(,CaCl2
R4748	T7727	T7725	parataxis	CaCl2,buffer
R4749	T7728	T7729	nummod	20,mM
R475	T1310	T1309	prep	by,differ
R4750	T7729	T7730	compound	mM,Hepes
R4751	T7730	T7727	dep	Hepes,CaCl2
R4752	T7731	T7730	punct	", ",Hepes
R4753	T7732	T7730	npadvmod	pH,Hepes
R4754	T7733	T7732	nummod	7.5,pH
R4755	T7734	T7727	punct	", ",CaCl2
R4756	T7735	T7736	nummod	150,mM
R4757	T7736	T7737	compound	mM,NaCl
R4758	T7737	T7727	dep	NaCl,CaCl2
R4759	T7738	T7727	punct	", ",CaCl2
R476	T1311	T1312	det	an,exon
R4760	T7739	T7740	nummod	1,mM
R4761	T7740	T7727	compound	mM,CaCl2
R4762	T7741	T7727	acl	adjusted,CaCl2
R4763	T7742	T7741	prep	to,adjusted
R4764	T7743	T7744	nummod	5,μM
R4765	T7744	T7745	compound	μM,A23187
R4766	T7745	T7742	pobj	A23187,to
R4767	T7746	T7745	compound	Ca2+,A23187
R4768	T7747	T7745	compound	ionophore,A23187
R4769	T7748	T7727	punct	),CaCl2
R477	T1312	T1310	pobj	exon,by
R4770	T7749	T7711	cc	and,combined
R4771	T7750	T7711	conj	incubated,combined
R4772	T7751	T7750	prep	for,incubated
R4773	T7752	T7753	nummod	30,minutes
R4774	T7753	T7751	pobj	minutes,for
R4775	T7754	T7750	prep	at,incubated
R4776	T7755	T7756	nummod	37,°C
R4777	T7756	T7754	pobj	°C,at
R4778	T7757	T7711	punct	.,combined
R4779	T7759	T7760	prep	After,added
R478	T1313	T1312	amod	additional,exon
R4780	T7761	T7759	pobj	incubation,After
R4781	T7762	T7760	nsubjpass	EDTA,added
R4782	T7763	T7760	auxpass	was,added
R4783	T7764	T7760	prep	to,added
R4784	T7765	T7766	det	the,solution
R4785	T7766	T7764	pobj	solution,to
R4786	T7767	T7760	prep	to,added
R4787	T7768	T7769	det	a,concentration
R4788	T7769	T7767	pobj	concentration,to
R4789	T7770	T7769	amod	final,concentration
R479	T1314	T1312	compound	cassette,exon
R4790	T7771	T7769	prep	of,concentration
R4791	T7772	T7773	nummod	5,mM
R4792	T7773	T7771	pobj	mM,of
R4793	T7774	T7760	cc	and,added
R4794	T7775	T7760	conj	centrifuged,added
R4795	T7776	T7775	prep	for,centrifuged
R4796	T7777	T7778	nummod	5,minutes
R4797	T7778	T7776	pobj	minutes,for
R4798	T7779	T7775	prep	at,centrifuged
R4799	T7780	T7781	nummod	400,g
R48	T260	T261	compound	membrane,fusion
R480	T1315	T1312	amod	located,exon
R4800	T7781	T7779	pobj	g,at
R4801	T7782	T7781	punct	×,g
R4802	T7783	T7781	cc	and,g
R4803	T7784	T7785	nummod	4,°C
R4804	T7785	T7781	conj	°C,g
R4805	T7786	T7760	punct	.,added
R4806	T7788	T7789	det	The,supernatant
R4807	T7789	T7790	nsubjpass	supernatant,collected
R4808	T7791	T7790	auxpass	was,collected
R4809	T7792	T7790	cc	and,collected
R481	T1316	T1315	prep	in,located
R4810	T7793	T7790	conj	centrifuged,collected
R4811	T7794	T7793	prep	for,centrifuged
R4812	T7795	T7796	nummod	20,minutes
R4813	T7796	T7794	pobj	minutes,for
R4814	T7797	T7793	prep	at,centrifuged
R4815	T7798	T7799	nummod	"15,000",g
R4816	T7799	T7797	pobj	g,at
R4817	T7800	T7799	punct	×,g
R4818	T7801	T7799	cc	and,g
R4819	T7802	T7803	nummod	4,°C
R482	T1317	T1318	det	the,third
R4820	T7803	T7799	conj	°C,g
R4821	T7804	T7790	punct	.,collected
R4822	T7806	T7807	det	The,pellet
R4823	T7807	T7809	nsubj	pellet,resuspended
R4824	T7808	T7807	amod	resulting,pellet
R4825	T7810	T7809	aux	was,resuspended
R4826	T7811	T7809	prep	in,resuspended
R4827	T7812	T7813	amod	washing,buffer
R4828	T7813	T7811	pobj	buffer,in
R4829	T7814	T7809	cc	and,resuspended
R483	T1318	T1316	pobj	third,in
R4830	T7815	T7809	conj	centrifuged,resuspended
R4831	T7816	T7815	prep	at,centrifuged
R4832	T7817	T7818	nummod	400,g
R4833	T7818	T7816	pobj	g,at
R4834	T7819	T7818	punct	×,g
R4835	T7820	T7815	advmod	again,centrifuged
R4836	T7821	T7822	aux	to,collect
R4837	T7822	T7809	advcl	collect,resuspended
R4838	T7823	T7824	det	a,supernatant
R4839	T7824	T7822	dobj	supernatant,collect
R484	T1319	T1318	amod	first,third
R4840	T7825	T7824	acl	enriched,supernatant
R4841	T7826	T7825	prep	with,enriched
R4842	T7827	T7828	amod	red,cell
R4843	T7828	T7830	compound	cell,microvesicles
R4844	T7829	T7828	compound	blood,cell
R4845	T7830	T7826	pobj	microvesicles,with
R4846	T7831	T7809	punct	.,resuspended
R4847	T7833	T7834	det	The,supernatant
R4848	T7834	T7835	nsubjpass	supernatant,centrifuged
R4849	T7836	T7834	prep	of,supernatant
R485	T1320	T1318	prep	of,third
R4850	T7837	T7838	det	the,step
R4851	T7838	T7836	pobj	step,of
R4852	T7839	T7840	nummod	"15,000",g
R4853	T7840	T7838	compound	g,step
R4854	T7841	T7840	punct	×,g
R4855	T7842	T7835	auxpass	was,centrifuged
R4856	T7843	T7835	advmod	further,centrifuged
R4857	T7844	T7835	prep	for,centrifuged
R4858	T7845	T7846	nummod	60,minutes
R4859	T7846	T7844	pobj	minutes,for
R486	T1321	T1322	det	the,domain
R4860	T7847	T7835	prep	at,centrifuged
R4861	T7848	T7849	nummod	"100,000",g
R4862	T7849	T7847	pobj	g,at
R4863	T7850	T7849	punct	×,g
R4864	T7851	T7849	cc	and,g
R4865	T7852	T7853	nummod	4,°C
R4866	T7853	T7849	conj	°C,g
R4867	T7854	T7835	punct	.,centrifuged
R4868	T7856	T7857	det	The,pellet
R4869	T7857	T7859	nsubj	pellet,resuspended
R487	T1322	T1320	pobj	domain,of
R4870	T7858	T7857	amod	final,pellet
R4871	T7860	T7857	prep	of,pellet
R4872	T7861	T7862	det	this,step
R4873	T7862	T7860	pobj	step,of
R4874	T7863	T7859	aux	was,resuspended
R4875	T7864	T7859	prep	in,resuspended
R4876	T7865	T7866	amod	washing,buffer
R4877	T7866	T7864	pobj	buffer,in
R4878	T7867	T7859	cc	and,resuspended
R4879	T7868	T7859	conj	centrifuged,resuspended
R488	T1323	T1324	npadvmod	N,terminal
R4880	T7869	T7868	prep	at,centrifuged
R4881	T7870	T7871	nummod	"15,000",g
R4882	T7871	T7869	pobj	g,at
R4883	T7872	T7871	punct	×,g
R4884	T7873	T7868	advmod	again,centrifuged
R4885	T7874	T7875	aux	to,purify
R4886	T7875	T7859	advcl	purify,resuspended
R4887	T7876	T7877	det	a,supernatant
R4888	T7877	T7875	dobj	supernatant,purify
R4889	T7878	T7877	acl	enriched,supernatant
R489	T1324	T1322	amod	terminal,domain
R4890	T7879	T7878	prep	in,enriched
R4891	T7880	T7879	pobj	nanovesicles,in
R4892	T7881	T7875	prep	according,purify
R4893	T7882	T7881	prep	to,according
R4894	T7883	T7882	punct	[,to
R4895	T7884	T7885	nummod	7,20
R4896	T7885	T7882	pobj	20,to
R4897	T7886	T7885	punct	",",20
R4898	T7887	T7859	punct	],resuspended
R4899	T7888	T7859	punct	.,resuspended
R49	T261	T262	compound	fusion,processes
R490	T1325	T1324	punct	-,terminal
R4900	T7890	T7891	det	The,vesicles
R4901	T7891	T7892	nsubjpass	vesicles,assayed
R4902	T7893	T7892	auxpass	were,assayed
R4903	T7894	T7892	prep	for,assayed
R4904	T7895	T7896	compound	acetylcholine,esterase
R4905	T7896	T7897	compound	esterase,activity
R4906	T7897	T7894	pobj	activity,for
R4907	T7898	T7892	cc	and,assayed
R4908	T7899	T7892	conj	used,assayed
R4909	T7900	T7899	prep	for,used
R491	T1326	T1309	punct	.,differ
R4910	T7901	T7902	amod	further,analysis
R4911	T7902	T7900	pobj	analysis,for
R4912	T7903	T7892	punct	.,assayed
R4913	T7951	T7952	compound	Acetylcholine,esterase
R4914	T7952	T7953	compound	esterase,assay
R4915	T7955	T7956	det	A,sample
R4916	T7956	T7959	nsubjpass	sample,mixed
R4917	T7957	T7958	nummod	30,μl
R4918	T7958	T7956	compound	μl,sample
R4919	T7960	T7956	prep	of,sample
R492	T1328	T1329	amod	Most,tissues
R4920	T7961	T7962	amod	red,cell
R4921	T7962	T7964	compound	cell,vesicles
R4922	T7963	T7962	compound	blood,cell
R4923	T7964	T7960	pobj	vesicles,of
R4924	T7965	T7959	auxpass	was,mixed
R4925	T7966	T7959	prep	with,mixed
R4926	T7967	T7968	det	an,volume
R4927	T7968	T7966	pobj	volume,with
R4928	T7969	T7968	amod	equal,volume
R4929	T7970	T7968	prep	of,volume
R493	T1329	T1330	nsubj	tissues,harbor
R4930	T7971	T7970	pobj	buffer,of
R4931	T7972	T7973	punct	(,X
R4932	T7973	T7971	parataxis	X,buffer
R4933	T7974	T7975	nummod	20,mM
R4934	T7975	T7976	compound	mM,Hepes
R4935	T7976	T7973	dep	Hepes,X
R4936	T7977	T7976	punct	", ",Hepes
R4937	T7978	T7976	npadvmod	pH,Hepes
R4938	T7979	T7978	nummod	7.6,pH
R4939	T7980	T7973	punct	", ",X
R494	T1330	T1331	ccomp	harbor,found
R4940	T7981	T7982	nummod	150,mM
R4941	T7982	T7983	compound	mM,NaCl
R4942	T7983	T7973	dep	NaCl,X
R4943	T7984	T7973	punct	", ",X
R4944	T7985	T7986	nummod	0.5,%
R4945	T7986	T7973	compound	%,X
R4946	T7987	T7973	compound	Triton,X
R4947	T7988	T7973	punct	-,X
R4948	T7989	T7973	nummod	100,X
R4949	T7990	T7973	punct	),X
R495	T1332	T1333	advmod	only,isoform
R4950	T7991	T7959	punct	", ",mixed
R4951	T7992	T7959	conj	vortexed,mixed
R4952	T7993	T7992	cc	and,vortexed
R4953	T7994	T7992	conj	incubated,vortexed
R4954	T7995	T7994	prep	for,incubated
R4955	T7996	T7997	nummod	5,minutes
R4956	T7997	T7995	pobj	minutes,for
R4957	T7998	T7994	prep	at,incubated
R4958	T7999	T8000	nummod	37,°C
R4959	T8000	T7998	pobj	°C,at
R496	T1333	T1330	dobj	isoform,harbor
R4960	T8001	T7959	punct	.,mixed
R4961	T8003	T8004	advmod	Subsequently,added
R4962	T8005	T8006	nummod	640,μl
R4963	T8006	T8007	compound	μl,buffer
R4964	T8007	T8004	nsubjpass	buffer,added
R4965	T8008	T8009	compound	sodium,phosphate
R4966	T8009	T8007	compound	phosphate,buffer
R4967	T8010	T8011	punct	(,Na2PO4
R4968	T8011	T8007	parataxis	Na2PO4,buffer
R4969	T8012	T8013	nummod	100,mM
R497	T1334	T1333	det	the,isoform
R4970	T8013	T8011	compound	mM,Na2PO4
R4971	T8014	T8011	punct	", ",Na2PO4
R4972	T8015	T8011	npadvmod	pH,Na2PO4
R4973	T8016	T8015	nummod	7.6,pH
R4974	T8017	T8011	punct	),Na2PO4
R4975	T8018	T8007	punct	", ",buffer
R4976	T8019	T8020	nummod	50,μl
R4977	T8020	T8021	compound	μl,DTNB
R4978	T8021	T8007	conj	DTNB,buffer
R4979	T8022	T8023	punct	(,mM
R498	T1335	T1336	nummod	47,kDa
R4980	T8023	T8021	parataxis	mM,DTNB
R4981	T8024	T8025	nummod	5,dithiobis
R4982	T8025	T8023	dep	dithiobis,mM
R4983	T8026	T8025	punct	",",dithiobis
R4984	T8027	T8025	nummod	5,dithiobis
R4985	T8028	T8027	punct	',5
R4986	T8029	T8025	punct	-,dithiobis
R4987	T8030	T8023	punct	-,mM
R4988	T8031	T8032	punct	(,acid
R4989	T8032	T8023	parataxis	acid,mM
R499	T1336	T1333	compound	kDa,isoform
R4990	T8033	T8032	nummod	2,acid
R4991	T8034	T8032	punct	-,acid
R4992	T8035	T8032	amod	nitrobenzoic,acid
R4993	T8036	T8032	punct	),acid
R4994	T8037	T8023	punct	", ",mM
R4995	T8038	T8023	nummod	10,mM
R4996	T8039	T8023	prep	in,mM
R4997	T8040	T8041	compound	sodium,phosphate
R4998	T8041	T8042	compound	phosphate,buffer
R4999	T8042	T8039	pobj	buffer,in
R5	T212	T205	prep	of,Function
R50	T262	T259	pobj	processes,in
R500	T1337	T1331	punct	", ",found
R5000	T8043	T8023	punct	),mM
R5001	T8044	T8021	punct	", ",DTNB
R5002	T8045	T8021	cc	and,DTNB
R5003	T8046	T8047	nummod	50,μl
R5004	T8047	T8048	compound	μl,chloride
R5005	T8048	T8021	conj	chloride,DTNB
R5006	T8049	T8048	compound	acetylthiocholine,chloride
R5007	T8050	T8051	punct	(,mM
R5008	T8051	T8048	parataxis	mM,chloride
R5009	T8052	T8051	nummod	12,mM
R501	T1338	T1339	det	both,forms
R5010	T8053	T8051	punct	",",mM
R5011	T8054	T8051	nummod	5,mM
R5012	T8055	T8051	prep	in,mM
R5013	T8056	T8055	pobj	H2O,in
R5014	T8057	T8051	punct	),mM
R5015	T8058	T8004	auxpass	were,added
R5016	T8059	T8004	punct	.,added
R5017	T8061	T8062	det	The,reaction
R5018	T8062	T8063	nsubj	reaction,took
R5019	T8064	T8063	dobj	place,took
R502	T1339	T1331	nsubjpass	forms,found
R5020	T8065	T8063	prep	at,took
R5021	T8066	T8067	compound	room,temperature
R5022	T8067	T8065	pobj	temperature,at
R5023	T8068	T8063	cc	and,took
R5024	T8069	T8070	auxpass	was,measured
R5025	T8070	T8063	conj	measured,took
R5026	T8071	T8070	advmod	photometrically,measured
R5027	T8072	T8070	prep	at,measured
R5028	T8073	T8074	det	a,wavelength
R5029	T8074	T8072	pobj	wavelength,at
R503	T1340	T1331	auxpass	are,found
R5030	T8075	T8074	prep	of,wavelength
R5031	T8076	T8077	nummod	405,nm
R5032	T8077	T8075	pobj	nm,of
R5033	T8078	T8070	prep	according,measured
R5034	T8079	T8078	prep	to,according
R5035	T8080	T8081	det	the,method
R5036	T8081	T8079	pobj	method,to
R5037	T8082	T8081	nmod	Ellmann,method
R5038	T8083	T8081	case	',method
R5039	T8084	T8085	punct	[,25
R504	T1341	T1331	prep	in,found
R5040	T8085	T8070	parataxis	25,measured
R5041	T8086	T8085	punct	],25
R5042	T8087	T8063	punct	.,took
R5043	T8114	T8113	prep	of,Determination
R5044	T8115	T8116	det	the,resistance
R5045	T8116	T8114	pobj	resistance,of
R5046	T8117	T8116	amod	osmotic,resistance
R5047	T8118	T8116	prep	of,resistance
R5048	T8119	T8120	amod	red,cells
R5049	T8120	T8118	pobj	cells,of
R505	T1342	T1341	pobj	brain,in
R5050	T8121	T8120	compound	blood,cells
R5051	T8123	T8124	det	A,gradient
R5052	T8124	T8130	nsubjpass	gradient,used
R5053	T8125	T8126	compound	Na2HPO4,NaH2PO4
R5054	T8126	T8128	npadvmod	NaH2PO4,buffered
R5055	T8127	T8126	punct	/,NaH2PO4
R5056	T8128	T8124	amod	buffered,gradient
R5057	T8129	T8124	compound	NaCl,gradient
R5058	T8131	T8132	punct	(,pH
R5059	T8132	T8124	npadvmod	pH,gradient
R506	T1343	T1342	cc	and,brain
R5060	T8133	T8132	nummod	7.4,pH
R5061	T8134	T8132	punct	),pH
R5062	T8135	T8124	acl	comprised,gradient
R5063	T8136	T8135	prep	of,comprised
R5064	T8137	T8138	det	the,steps
R5065	T8138	T8136	pobj	steps,of
R5066	T8139	T8138	amod	following,steps
R5067	T8140	T8138	compound	NaCl,steps
R5068	T8141	T8130	auxpass	was,used
R5069	T8142	T8130	prep	for,used
R507	T1344	T1342	conj	heart,brain
R5070	T8143	T8144	det	this,assay
R5071	T8144	T8142	pobj	assay,for
R5072	T8145	T8130	punct	: ,used
R5073	T8146	T8147	nummod	0.90,%
R5074	T8147	T8130	dobj	%,used
R5075	T8148	T8147	punct	", ",%
R5076	T8149	T8150	nummod	0.65,%
R5077	T8150	T8147	appos	%,%
R5078	T8151	T8147	punct	", ",%
R5079	T8152	T8153	nummod	0.60,%
R508	T1345	T1331	punct	", ",found
R5080	T8153	T8147	appos	%,%
R5081	T8154	T8147	punct	", ",%
R5082	T8155	T8156	nummod	0.55,%
R5083	T8156	T8147	appos	%,%
R5084	T8157	T8147	punct	", ",%
R5085	T8158	T8159	nummod	0.50,%
R5086	T8159	T8147	appos	%,%
R5087	T8160	T8147	punct	", ",%
R5088	T8161	T8162	nummod	0.45,%
R5089	T8162	T8147	appos	%,%
R509	T1346	T1347	prep	while,expressed
R5090	T8163	T8147	punct	", ",%
R5091	T8164	T8165	nummod	0.40,%
R5092	T8165	T8147	appos	%,%
R5093	T8166	T8147	punct	", ",%
R5094	T8167	T8168	nummod	0.35,%
R5095	T8168	T8147	appos	%,%
R5096	T8169	T8147	punct	", ",%
R5097	T8170	T8171	nummod	0.30,%
R5098	T8171	T8147	appos	%,%
R5099	T8172	T8147	punct	", ",%
R51	T263	T235	punct	.,is
R510	T1347	T1331	advcl	expressed,found
R5100	T8173	T8174	nummod	0.10,%
R5101	T8174	T8147	appos	%,%
R5102	T8175	T8130	punct	.,used
R5103	T8177	T8178	prep	For,inverted
R5104	T8179	T8180	det	each,step
R5105	T8180	T8177	pobj	step,For
R5106	T8181	T8182	nummod	5,ml
R5107	T8182	T8178	nsubjpass	ml,inverted
R5108	T8183	T8182	prep	of,ml
R5109	T8184	T8185	det	the,solution
R511	T1348	T1349	det	the,isoform
R5110	T8185	T8183	pobj	solution,of
R5111	T8186	T8185	compound	gradient,solution
R5112	T8187	T8185	compound	step,solution
R5113	T8188	T8182	cc	and,ml
R5114	T8189	T8190	nummod	50,μl
R5115	T8190	T8191	compound	μl,sample
R5116	T8191	T8182	conj	sample,ml
R5117	T8192	T8191	compound	blood,sample
R5118	T8193	T8178	auxpass	were,inverted
R5119	T8194	T8178	advmod	carefully,inverted
R512	T1349	T1347	nsubjpass	isoform,expressed
R5120	T8195	T8196	nummod	four,times
R5121	T8196	T8178	npadvmod	times,inverted
R5122	T8197	T8178	cc	and,inverted
R5123	T8198	T8178	conj	incubated,inverted
R5124	T8199	T8198	prep	for,incubated
R5125	T8200	T8201	nummod	30,minutes
R5126	T8201	T8199	pobj	minutes,for
R5127	T8202	T8198	prep	in,incubated
R5128	T8203	T8204	det	the,dark
R5129	T8204	T8202	pobj	dark,in
R513	T1350	T1349	amod	large,isoform
R5130	T8205	T8178	punct	.,inverted
R5131	T8207	T8208	nsubjpass	Samples,inverted
R5132	T8209	T8208	auxpass	were,inverted
R5133	T8210	T8208	advmod	carefully,inverted
R5134	T8211	T8208	advmod	again,inverted
R5135	T8212	T8208	cc	and,inverted
R5136	T8213	T8208	conj	centrifuged,inverted
R5137	T8214	T8213	prep	at,centrifuged
R5138	T8215	T8216	nummod	"1,500",g
R5139	T8216	T8214	pobj	g,at
R514	T1351	T1347	auxpass	is,expressed
R5140	T8217	T8216	punct	×,g
R5141	T8218	T8213	prep	for,centrifuged
R5142	T8219	T8220	nummod	10,minutes
R5143	T8220	T8218	pobj	minutes,for
R5144	T8221	T8208	punct	.,inverted
R5145	T8223	T8224	prep	From,measured
R5146	T8225	T8226	nummod	1,ml
R5147	T8226	T8227	compound	ml,supernatant
R5148	T8227	T8223	pobj	supernatant,From
R5149	T8228	T8227	prep	of,supernatant
R515	T1352	T1347	advmod	exclusively,expressed
R5150	T8229	T8230	det	each,step
R5151	T8230	T8228	pobj	step,of
R5152	T8231	T8232	det	the,extinction
R5153	T8232	T8224	nsubjpass	extinction,measured
R5154	T8233	T8232	prep	at,extinction
R5155	T8234	T8235	nummod	546,nm
R5156	T8235	T8236	compound	nm,wavelength
R5157	T8236	T8233	pobj	wavelength,at
R5158	T8237	T8224	auxpass	was,measured
R5159	T8238	T8224	punct	.,measured
R516	T1353	T1347	prep	in,expressed
R5160	T8240	T8241	det	The,experiment
R5161	T8241	T8242	nsubjpass	experiment,performed
R5162	T8243	T8242	auxpass	was,performed
R5163	T8244	T8242	prep	at,performed
R5164	T8245	T8246	compound	room,temperature
R5165	T8246	T8244	pobj	temperature,at
R5166	T8247	T8242	punct	.,performed
R5167	T8326	T8327	compound	Platelet,aggregation
R5168	T8327	T8328	compound	aggregation,assay
R5169	T8330	T8331	advmod	Approximately,500
R517	T1354	T1355	amod	mature,muscle
R5170	T8331	T8332	nummod	500,μl
R5171	T8332	T8333	nsubjpass	μl,centrifuged
R5172	T8334	T8332	prep	of,μl
R5173	T8335	T8336	amod	murine,blood
R5174	T8336	T8334	pobj	blood,of
R5175	T8337	T8333	auxpass	were,centrifuged
R5176	T8338	T8333	prep	at,centrifuged
R5177	T8339	T8340	nummod	200,g
R5178	T8340	T8338	pobj	g,at
R5179	T8341	T8340	punct	×,g
R518	T1355	T1353	pobj	muscle,in
R5180	T8342	T8333	prep	for,centrifuged
R5181	T8343	T8344	nummod	15,minutes
R5182	T8344	T8342	pobj	minutes,for
R5183	T8345	T8333	punct	.,centrifuged
R5184	T8347	T8348	det	The,supernatants
R5185	T8348	T8350	nsubjpass	supernatants,transferred
R5186	T8349	T8348	amod	resulting,supernatants
R5187	T8351	T8350	auxpass	were,transferred
R5188	T8352	T8350	prep	to,transferred
R5189	T8353	T8354	amod	new,tubes
R519	T1356	T1355	amod	skeletal,muscle
R5190	T8354	T8352	pobj	tubes,to
R5191	T8355	T8350	cc	and,transferred
R5192	T8356	T8350	conj	used,transferred
R5193	T8357	T8356	prep	as,used
R5194	T8358	T8359	npadvmod	platelet,rich
R5195	T8359	T8360	amod	rich,plasma
R5196	T8360	T8357	pobj	plasma,as
R5197	T8361	T8360	punct	(,plasma
R5198	T8362	T8360	appos	PRP,plasma
R5199	T8363	T8350	punct	),transferred
R52	T265	T266	advmod	Recently,identified
R520	T1357	T1331	punct	.,found
R5200	T8364	T8350	punct	.,transferred
R5201	T8366	T8367	det	The,measurements
R5202	T8367	T8370	nsubjpass	measurements,done
R5203	T8368	T8369	compound	platelet,aggregation
R5204	T8369	T8367	compound	aggregation,measurements
R5205	T8371	T8370	auxpass	were,done
R5206	T8372	T8370	prep	according,done
R5207	T8373	T8372	prep	to,according
R5208	T8374	T8375	det	the,method
R5209	T8375	T8373	pobj	method,to
R521	T1359	T1360	prep	At,detected
R5210	T8376	T8375	acl	developed,method
R5211	T8377	T8376	agent	by,developed
R5212	T8378	T8377	pobj	Born,by
R5213	T8379	T8378	cc	and,Born
R5214	T8380	T8378	conj	Cross,Born
R5215	T8381	T8382	punct	[,26
R5216	T8382	T8380	parataxis	26,Cross
R5217	T8383	T8382	punct	],26
R5218	T8384	T8370	advcl	using,done
R5219	T8385	T8386	det	an,photometer
R522	T1361	T1362	det	the,level
R5220	T8386	T8384	dobj	photometer,using
R5221	T8387	T8386	compound	APACT,photometer
R5222	T8388	T8389	punct	(,FRG
R5223	T8389	T8386	parataxis	FRG,photometer
R5224	T8390	T8389	dep	Labor,FRG
R5225	T8391	T8389	punct	", ",FRG
R5226	T8392	T8389	dep	Ahrendsburg,FRG
R5227	T8393	T8389	punct	", ",FRG
R5228	T8394	T8389	punct	),FRG
R5229	T8395	T8386	cc	and,photometer
R523	T1362	T1359	pobj	level,At
R5230	T8396	T8397	det	the,software
R5231	T8397	T8386	conj	software,photometer
R5232	T8398	T8397	compound	APACT,software
R5233	T8399	T8400	punct	(,APACT
R5234	T8400	T8397	parataxis	APACT,software
R5235	T8401	T8400	nummod	1.4,APACT
R5236	T8402	T8400	punct	),APACT
R5237	T8403	T8370	punct	.,done
R5238	T8405	T8406	advmod	Subsequently,adjusted
R5239	T8407	T8406	nsubjpass	samples,adjusted
R524	T1363	T1362	amod	cellular,level
R5240	T8408	T8407	prep	of,samples
R5241	T8409	T8410	det	the,PRP
R5242	T8410	T8408	pobj	PRP,of
R5243	T8411	T8406	auxpass	were,adjusted
R5244	T8412	T8406	prep	with,adjusted
R5245	T8413	T8412	pobj	buffer,with
R5246	T8414	T8415	punct	(,pH
R5247	T8415	T8413	parataxis	pH,buffer
R5248	T8416	T8417	nummod	5,mM
R5249	T8417	T8418	compound	mM,Hepes
R525	T1364	T1365	compound	annexin,A7
R5250	T8418	T8415	dep	Hepes,pH
R5251	T8419	T8415	punct	", ",pH
R5252	T8420	T8421	nummod	150,mM
R5253	T8421	T8422	compound	mM,NaCl
R5254	T8422	T8415	dep	NaCl,pH
R5255	T8423	T8415	punct	", ",pH
R5256	T8424	T8415	nummod	7.3,pH
R5257	T8425	T8415	punct	),pH
R5258	T8426	T8406	prep	to,adjusted
R5259	T8427	T8428	nummod	"250,000",platelets
R526	T1365	T1360	nsubjpass	A7,detected
R5260	T8428	T8426	pobj	platelets,to
R5261	T8429	T8430	punct	/,μl
R5262	T8430	T8428	prep	μl,platelets
R5263	T8431	T8406	punct	.,adjusted
R5264	T8433	T8434	det	A,count
R5265	T8434	T8437	nsubjpass	count,used
R5266	T8435	T8434	amod	constant,count
R5267	T8436	T8434	compound	platelet,count
R5268	T8438	T8437	auxpass	was,used
R5269	T8439	T8437	advmod	throughout,used
R527	T1366	T1360	aux	can,detected
R5270	T8440	T8437	advcl	allowing,used
R5271	T8441	T8440	dobj	comparisons,allowing
R5272	T8442	T8441	prep	between,comparisons
R5273	T8443	T8444	amod	wild,type
R5274	T8444	T8445	nmod	type,samples
R5275	T8445	T8442	pobj	samples,between
R5276	T8446	T8444	cc	and,type
R5277	T8447	T8448	compound	knock,out
R5278	T8448	T8444	conj	out,type
R5279	T8449	T8445	amod	murine,samples
R528	T1367	T1360	auxpass	be,detected
R5280	T8450	T8449	cc	and,murine
R5281	T8451	T8449	conj	human,murine
R5282	T8452	T8437	punct	.,used
R5283	T8454	T8455	nummod	250,μl
R5284	T8455	T8456	nmod	μl,platelets
R5285	T8456	T8458	nsubjpass	platelets,prewarmed
R5286	T8457	T8456	amod	adjusted,platelets
R5287	T8459	T8458	auxpass	were,prewarmed
R5288	T8460	T8458	prep	to,prewarmed
R5289	T8461	T8462	nummod	37,°C
R529	T1368	T1360	prep	in,detected
R5290	T8462	T8460	pobj	°C,to
R5291	T8463	T8458	cc	and,prewarmed
R5292	T8464	T8458	conj	used,prewarmed
R5293	T8465	T8464	prep	in,used
R5294	T8466	T8467	det	the,assay
R5295	T8467	T8465	pobj	assay,in
R5296	T8468	T8458	punct	", ",prewarmed
R5297	T8469	T8458	cc	and,prewarmed
R5298	T8470	T8471	det	the,process
R5299	T8471	T8473	nsubjpass	process,started
R53	T267	T268	det	the,isoform
R530	T1369	T1370	det	the,cytosol
R5300	T8472	T8471	compound	aggregation,process
R5301	T8473	T8458	conj	started,prewarmed
R5302	T8474	T8473	auxpass	was,started
R5303	T8475	T8473	prep	by,started
R5304	T8476	T8475	pcomp	adding,by
R5305	T8477	T8478	nummod	25,μl
R5306	T8478	T8476	dobj	μl,adding
R5307	T8479	T8478	prep	of,μl
R5308	T8480	T8481	det	a,solution
R5309	T8481	T8479	pobj	solution,of
R531	T1370	T1368	pobj	cytosol,in
R5310	T8482	T8481	amod	prewarmed,solution
R5311	T8483	T8484	nummod	16.5,mg
R5312	T8484	T8481	nmod	mg,solution
R5313	T8485	T8486	punct	/,ml
R5314	T8486	T8484	prep	ml,mg
R5315	T8487	T8481	compound	ristocetin,solution
R5316	T8488	T8489	punct	(,FRG
R5317	T8489	T8481	parataxis	FRG,solution
R5318	T8490	T8491	compound	DiaMed,Diagnostica
R5319	T8491	T8489	dep	Diagnostica,FRG
R532	T1371	T1368	punct	", ",in
R5320	T8492	T8489	punct	", ",FRG
R5321	T8493	T8489	dep	Bensheim,FRG
R5322	T8494	T8489	punct	", ",FRG
R5323	T8495	T8489	punct	;,FRG
R5324	T8496	T8497	dep	concentration,mg
R5325	T8497	T8489	dep	mg,FRG
R5326	T8498	T8496	prep	in,concentration
R5327	T8499	T8500	det	the,assay
R5328	T8500	T8498	pobj	assay,in
R5329	T8501	T8497	punct	: ,mg
R533	T1372	T1368	conj	at,in
R5330	T8502	T8497	nummod	1.5,mg
R5331	T8503	T8504	punct	/,ml
R5332	T8504	T8497	prep	ml,mg
R5333	T8505	T8489	punct	),FRG
R5334	T8506	T8481	punct	", ",solution
R5335	T8507	T8508	det	a,reagent
R5336	T8508	T8481	appos	reagent,solution
R5337	T8509	T8510	dep	which,worked
R5338	T8510	T8508	relcl	worked,reagent
R5339	T8511	T8510	advmod	reliably,worked
R534	T1373	T1374	det	the,membrane
R5340	T8512	T8510	prep	in,worked
R5341	T8513	T8514	poss	our,hands
R5342	T8514	T8512	pobj	hands,in
R5343	T8515	T8510	prep	with,worked
R5344	T8516	T8517	nmod	mouse,platelets
R5345	T8517	T8515	pobj	platelets,with
R5346	T8518	T8516	cc	and,mouse
R5347	T8519	T8516	conj	human,mouse
R5348	T8520	T8473	punct	.,started
R5349	T8522	T8523	det	Each,assay
R535	T1374	T1372	pobj	membrane,at
R5350	T8523	T8525	nsubj	assay,represents
R5351	T8524	T8523	amod	murine,assay
R5352	T8526	T8527	det	a,animal
R5353	T8527	T8525	dobj	animal,represents
R5354	T8528	T8527	amod	different,animal
R5355	T8529	T8525	punct	.,represents
R5356	T8531	T8532	nsubjpass	Analyses,performed
R5357	T8533	T8531	prep	of,Analyses
R5358	T8534	T8535	amod	human,samples
R5359	T8535	T8533	pobj	samples,of
R536	T1375	T1374	compound	plasma,membrane
R5360	T8536	T8535	compound	blood,samples
R5361	T8537	T8532	auxpass	were,performed
R5362	T8538	T8539	mark	as,described
R5363	T8539	T8532	advcl	described,performed
R5364	T8540	T8539	prep	in,described
R5365	T8541	T8542	det	the,manufacturer
R5366	T8542	T8543	poss	manufacturer,protocol
R5367	T8543	T8540	pobj	protocol,in
R5368	T8544	T8542	case	's,manufacturer
R5369	T8545	T8532	punct	.,performed
R537	T1376	T1372	punct	", ",at
R5370	T8547	T8548	amod	Several,measurements
R5371	T8548	T8550	nsubjpass	measurements,done
R5372	T8549	T8548	amod	repeated,measurements
R5373	T8551	T8550	auxpass	were,done
R5374	T8552	T8550	prep	per,done
R5375	T8553	T8554	amod	human,individual
R5376	T8554	T8552	pobj	individual,per
R5377	T8555	T8556	aux	to,control
R5378	T8556	T8550	advcl	control,done
R5379	T8557	T8558	det	the,reproducibility
R538	T1377	T1372	conj	around,at
R5380	T8558	T8556	dobj	reproducibility,control
R5381	T8559	T8550	punct	.,done
R5382	T8561	T8562	det	The,data
R5383	T8562	T8566	nsubjpass	data,analysed
R5384	T8563	T8562	amod	acquired,data
R5385	T8564	T8565	compound	aggregation,curve
R5386	T8565	T8562	compound	curve,data
R5387	T8567	T8562	punct	", ",data
R5388	T8568	T8562	acl	measured,data
R5389	T8569	T8568	prep	as,measured
R539	T1378	T1379	det	the,nucleus
R5390	T8570	T8571	amod	ascending,degree
R5391	T8571	T8569	pobj	degree,as
R5392	T8572	T8571	prep	of,degree
R5393	T8573	T8572	pobj	transmission,of
R5394	T8574	T8566	punct	", ",analysed
R5395	T8575	T8566	auxpass	were,analysed
R5396	T8576	T8566	prep	for,analysed
R5397	T8577	T8578	amod	maximal,values
R5398	T8578	T8576	pobj	values,for
R5399	T8579	T8578	compound	slope,values
R54	T268	T266	nsubjpass	isoform,identified
R540	T1379	T1377	pobj	nucleus,around
R5400	T8580	T8566	punct	", ",analysed
R5401	T8581	T8582	prep	while,set
R5402	T8582	T8566	advcl	set,analysed
R5403	T8583	T8584	det	the,amplitude
R5404	T8584	T8582	nsubjpass	amplitude,set
R5405	T8585	T8584	amod	maximal,amplitude
R5406	T8586	T8584	compound	aggregation,amplitude
R5407	T8587	T8584	prep	of,amplitude
R5408	T8588	T8589	det	every,curve
R5409	T8589	T8587	pobj	curve,of
R541	T1380	T1377	punct	", ",around
R5410	T8590	T8589	amod	single,curve
R5411	T8591	T8582	auxpass	was,set
R5412	T8592	T8582	prep	to,set
R5413	T8593	T8594	nummod	100,%
R5414	T8594	T8592	pobj	%,to
R5415	T8595	T8566	punct	.,analysed
R5416	T8597	T8598	prep	In,done
R5417	T8599	T8597	amod	general,In
R5418	T8600	T8598	punct	", ",done
R5419	T8601	T8602	det	all,procedures
R542	T1381	T1377	conj	at,around
R5420	T8602	T8598	nsubjpass	procedures,done
R5421	T8603	T8602	compound	platelet,procedures
R5422	T8604	T8598	auxpass	were,done
R5423	T8605	T8598	prep	at,done
R5424	T8606	T8607	nummod	22,°C
R5425	T8607	T8605	pobj	°C,at
R5426	T8608	T8598	prep	within,done
R5427	T8609	T8610	nummod	four,hours
R5428	T8610	T8608	pobj	hours,within
R5429	T8611	T8610	prep	after,hours
R543	T1382	T1383	amod	vesicular,structures
R5430	T8612	T8611	pcomp	collecting,after
R5431	T8613	T8614	det	the,sample
R5432	T8614	T8612	dobj	sample,collecting
R5433	T8615	T8614	compound	blood,sample
R5434	T8616	T8598	punct	.,done
R5435	T8735	T8736	amod	Miscellaneous,methods
R5436	T8738	T8739	prep	For,analysed
R5437	T8740	T8741	amod	standard,measurements
R5438	T8741	T8738	pobj	measurements,For
R5439	T8742	T8741	amod	haematopoetic,measurements
R544	T1383	T1381	pobj	structures,at
R5440	T8743	T8741	cc	and,measurements
R5441	T8744	T8745	compound	cell,counts
R5442	T8745	T8741	conj	counts,measurements
R5443	T8746	T8741	prep	on,measurements
R5444	T8747	T8748	nmod	murine,samples
R5445	T8748	T8746	pobj	samples,on
R5446	T8749	T8747	cc	and,murine
R5447	T8750	T8747	conj	human,murine
R5448	T8751	T8748	compound	blood,samples
R5449	T8752	T8753	quantmod	about,300
R545	T1384	T1383	prep	including,structures
R5450	T8753	T8754	nummod	300,μl
R5451	T8754	T8739	nsubjpass	μl,analysed
R5452	T8755	T8754	prep	of,μl
R5453	T8756	T8755	pobj	blood,of
R5454	T8757	T8739	auxpass	were,analysed
R5455	T8758	T8739	prep	with,analysed
R5456	T8759	T8760	det	an,counter
R5457	T8760	T8758	pobj	counter,with
R5458	T8761	T8760	nmod	ADVIA,counter
R5459	T8762	T8761	nummod	120,ADVIA
R546	T1385	T1386	amod	adrenal,granules
R5460	T8763	T8760	amod	electronic,counter
R5461	T8764	T8760	compound	cell,counter
R5462	T8765	T8766	punct	(,Bayer
R5463	T8766	T8760	parataxis	Bayer,counter
R5464	T8767	T8766	punct	),Bayer
R5465	T8768	T8739	punct	.,analysed
R5466	T8770	T8771	prep	With,transformed
R5467	T8772	T8773	det	this,equipment
R5468	T8773	T8770	pobj	equipment,With
R5469	T8774	T8775	amod	red,cells
R547	T1386	T1384	pobj	granules,including
R5470	T8775	T8771	nsubjpass	cells,transformed
R5471	T8776	T8775	compound	blood,cells
R5472	T8777	T8775	cc	and,cells
R5473	T8778	T8775	conj	platelets,cells
R5474	T8779	T8771	auxpass	are,transformed
R5475	T8780	T8771	punct	"""",transformed
R5476	T8781	T8771	punct	"""",transformed
R5477	T8782	T8771	prep	into,transformed
R5478	T8783	T8784	amod	spherical,bodies
R5479	T8784	T8782	pobj	bodies,into
R548	T1387	T1386	compound	chromaffin,granules
R5480	T8785	T8771	prep	without,transformed
R5481	T8786	T8785	pcomp	changing,without
R5482	T8787	T8788	poss	their,volume
R5483	T8788	T8786	dobj	volume,changing
R5484	T8789	T8771	punct	.,transformed
R5485	T8791	T8792	advmod	Therefore,are
R5486	T8793	T8792	nsubj	number,are
R5487	T8794	T8793	cc	and,number
R5488	T8795	T8793	conj	volume,number
R5489	T8796	T8793	prep	of,number
R549	T1388	T1381	punct	", ",at
R5490	T8797	T8798	amod	red,cells
R5491	T8798	T8796	pobj	cells,of
R5492	T8799	T8798	compound	blood,cells
R5493	T8800	T8798	cc	and,cells
R5494	T8801	T8798	conj	platelets,cells
R5495	T8802	T8792	neg	not,are
R5496	T8803	T8804	amod	calculated,values
R5497	T8804	T8792	attr	values,are
R5498	T8805	T8792	punct	", ",are
R5499	T8806	T8792	cc	but,are
R55	T269	T270	nummod	47,kDa
R550	T1389	T1381	cc	and,at
R5500	T8807	T8808	advmod	directly,measured
R5501	T8808	T8809	amod	measured,values
R5502	T8809	T8792	conj	values,are
R5503	T8810	T8792	punct	.,are
R5504	T8812	T8813	det	The,data
R5505	T8813	T8815	nsubjpass	data,analysed
R5506	T8814	T8813	amod	following,data
R5507	T8816	T8815	auxpass	were,analysed
R5508	T8817	T8815	advmod	statistically,analysed
R5509	T8818	T8815	punct	: ,analysed
R551	T1390	T1381	conj	at,at
R5510	T8819	T8815	dobj	leucocytes,analysed
R5511	T8820	T8819	punct	", ",leucocytes
R5512	T8821	T8822	amod	red,cells
R5513	T8822	T8819	appos	cells,leucocytes
R5514	T8823	T8822	compound	blood,cells
R5515	T8824	T8819	punct	", ",leucocytes
R5516	T8825	T8819	appos	haemoglobin,leucocytes
R5517	T8826	T8819	punct	", ",leucocytes
R5518	T8827	T8819	appos	haematocrit,leucocytes
R5519	T8828	T8819	punct	", ",leucocytes
R552	T1391	T1392	det	the,system
R5520	T8829	T8830	amod	mean,volume
R5521	T8830	T8819	appos	volume,leucocytes
R5522	T8831	T8830	amod	cellular,volume
R5523	T8832	T8819	punct	", ",leucocytes
R5524	T8833	T8834	amod	mean,haemoglobin
R5525	T8834	T8819	appos	haemoglobin,leucocytes
R5526	T8835	T8834	amod	cellular,haemoglobin
R5527	T8836	T8819	punct	", ",leucocytes
R5528	T8837	T8838	amod	mean,haemoglobin
R5529	T8838	T8840	compound	haemoglobin,concentration
R553	T1392	T1390	pobj	system,at
R5530	T8839	T8838	amod	cellular,haemoglobin
R5531	T8840	T8819	appos	concentration,leucocytes
R5532	T8841	T8819	punct	", ",leucocytes
R5533	T8842	T8843	compound	platelet,number
R5534	T8843	T8819	appos	number,leucocytes
R5535	T8844	T8819	punct	", ",leucocytes
R5536	T8845	T8819	appos	neutrophiles,leucocytes
R5537	T8846	T8819	punct	", ",leucocytes
R5538	T8847	T8819	appos	lymphocytes,leucocytes
R5539	T8848	T8819	punct	", ",leucocytes
R554	T1393	T1394	compound	t,tubule
R5540	T8849	T8819	appos	monocytes,leucocytes
R5541	T8850	T8819	punct	", ",leucocytes
R5542	T8851	T8819	appos	eosinophiles,leucocytes
R5543	T8852	T8819	punct	", ",leucocytes
R5544	T8853	T8819	appos	basophiles,leucocytes
R5545	T8854	T8815	punct	.,analysed
R5546	T8856	T8857	compound	Peptide,mass
R5547	T8857	T8858	compound	mass,fingerprinting
R5548	T8858	T8859	nsubjpass	fingerprinting,performed
R5549	T8860	T8859	auxpass	was,performed
R555	T1394	T1392	compound	tubule,system
R5550	T8861	T8859	prep	on,performed
R5551	T8862	T8863	det	a,spectrometer
R5552	T8863	T8861	pobj	spectrometer,on
R5553	T8864	T8865	nmod	Bruker,Reflex
R5554	T8865	T8863	nmod	Reflex,spectrometer
R5555	T8866	T8865	nummod	IV,Reflex
R5556	T8867	T8868	compound	MALDI,TOF
R5557	T8868	T8863	compound	TOF,spectrometer
R5558	T8869	T8868	punct	-,TOF
R5559	T8870	T8863	compound	mass,spectrometer
R556	T1395	T1394	punct	-,tubule
R5560	T8871	T8859	punct	.,performed
R5561	T8873	T8874	amod	Recombinant,A7
R5562	T8874	T8876	nsubjpass	A7,purified
R5563	T8875	T8874	compound	annexin,A7
R5564	T8876	T8878	ccomp	purified,done
R5565	T8877	T8876	auxpass	was,purified
R5566	T8879	T8876	prep	according,purified
R5567	T8880	T8879	prep	to,according
R5568	T8881	T8880	punct	[,to
R5569	T8882	T8880	pobj	27,to
R557	T1396	T1397	punct	[,6
R5570	T8883	T8878	punct	],done
R5571	T8884	T8878	punct	", ",done
R5572	T8885	T8886	compound	F,actin
R5573	T8886	T8878	nsubjpass	actin,done
R5574	T8887	T8886	punct	-,actin
R5575	T8888	T8886	punct	-,actin
R5576	T8889	T8886	amod	binding,actin
R5577	T8890	T8886	prep	of,actin
R5578	T8891	T8892	amod	recombinant,A7
R5579	T8892	T8890	pobj	A7,of
R558	T1397	T1360	parataxis	6,detected
R5580	T8893	T8892	compound	annexin,A7
R5581	T8894	T8878	auxpass	was,done
R5582	T8895	T8896	mark	as,described
R5583	T8896	T8878	advcl	described,done
R5584	T8897	T8898	punct	[,28
R5585	T8898	T8878	parataxis	28,done
R5586	T8899	T8898	punct	],28
R5587	T8900	T8878	punct	.,done
R5588	T8902	T8903	compound	SDS,PAGE
R5589	T8903	T8905	nsubjpass	PAGE,done
R559	T1398	T1397	nummod	5,6
R5590	T8904	T8903	punct	-,PAGE
R5591	T8906	T8903	cc	and,PAGE
R5592	T8907	T8908	amod	western,blotting
R5593	T8908	T8903	conj	blotting,PAGE
R5594	T8909	T8905	auxpass	were,done
R5595	T8910	T8911	mark	as,described
R5596	T8911	T8905	advcl	described,done
R5597	T8912	T8913	punct	[,30
R5598	T8913	T8905	parataxis	30,done
R5599	T8914	T8913	nummod	29,30
R56	T270	T268	compound	kDa,isoform
R560	T1399	T1397	punct	",",6
R5600	T8915	T8913	punct	",",30
R5601	T8916	T8913	punct	],30
R5602	T8917	T8905	punct	.,done
R5603	T8919	T8920	nsubj	Detection,was
R5604	T8921	T8919	prep	in,Detection
R5605	T8922	T8921	pobj	immunoblots,in
R5606	T8923	T8920	prep	with,was
R5607	T8924	T8925	amod	enhanced,chemiluminescence
R5608	T8925	T8923	pobj	chemiluminescence,with
R5609	T8926	T8927	punct	[,17
R561	T1400	T1397	punct	],6
R5610	T8927	T8920	parataxis	17,was
R5611	T8928	T8927	punct	],17
R5612	T8929	T8920	punct	.,was
R5613	T8931	T8932	nsubj	Antibodies,were
R5614	T8933	T8931	acl	employed,Antibodies
R5615	T8934	T8935	nmod	mab,203
R5616	T8935	T8932	attr	203,were
R5617	T8936	T8937	punct	–,217
R5618	T8937	T8935	prep	217,203
R5619	T8938	T8935	acl	directed,203
R562	T1401	T1360	punct	.,detected
R5620	T8939	T8938	prep	against,directed
R5621	T8940	T8941	compound	mouse,domain
R5622	T8941	T8939	pobj	domain,against
R5623	T8942	T8943	compound	annexin,A7
R5624	T8943	T8941	compound	A7,domain
R5625	T8944	T8941	compound	core,domain
R5626	T8945	T8946	punct	[,5
R5627	T8946	T8941	parataxis	5,domain
R5628	T8947	T8946	punct	],5
R5629	T8948	T8941	cc	and,domain
R563	T1403	T1404	compound	Annexin,A7
R5630	T8949	T8950	det	a,antibody
R5631	T8950	T8941	conj	antibody,domain
R5632	T8951	T8950	amod	polyclonal,antibody
R5633	T8952	T8950	nmod	rabbit,antibody
R5634	T8953	T8950	amod	anti-sorcin,antibody
R5635	T8954	T8955	punct	[,31
R5636	T8955	T8950	parataxis	31,antibody
R5637	T8956	T8955	punct	],31
R5638	T8957	T8932	punct	.,were
R5639	T8959	T8960	prep	For,performed
R564	T1404	T1405	nsubj	A7,translocates
R5640	T8961	T8962	amod	statistical,analyses
R5641	T8962	T8959	pobj	analyses,For
R5642	T8963	T8964	det	the,tests
R5643	T8964	T8960	nsubjpass	tests,performed
R5644	T8965	T8964	amod	following,tests
R5645	T8966	T8960	auxpass	were,performed
R5646	T8967	T8960	punct	: ,performed
R5647	T8968	T8969	nmod	David,test
R5648	T8969	T8960	dobj	test,performed
R5649	T8970	T8971	advmod	et,al.
R565	T1406	T1405	prep	to,translocates
R5650	T8971	T8968	advmod	al.,David
R5651	T8972	T8969	compound	range,test
R5652	T8973	T8974	aux	to,ensure
R5653	T8974	T8969	advcl	ensure,test
R5654	T8975	T8976	nsubj	samples,are
R5655	T8976	T8974	advcl	are,ensure
R5656	T8977	T8976	prep	from,are
R5657	T8978	T8979	det	a,distribution
R5658	T8979	T8977	pobj	distribution,from
R5659	T8980	T8979	amod	normal,distribution
R566	T1407	T1406	pobj	membranes,to
R5660	T8981	T8969	punct	", ",test
R5661	T8982	T8983	compound	F,test
R5662	T8983	T8969	appos	test,test
R5663	T8984	T8983	punct	-,test
R5664	T8985	T8983	acl	looking,test
R5665	T8986	T8985	prep	for,looking
R5666	T8987	T8988	det	a,samples
R5667	T8988	T8989	poss	samples,homoscedascity
R5668	T8989	T8986	pobj	homoscedascity,for
R5669	T8990	T8988	case	',samples
R567	T1408	T1405	prep	in,translocates
R5670	T8991	T8969	punct	", ",test
R5671	T8992	T8993	poss	Student,test
R5672	T8993	T8969	appos	test,test
R5673	T8994	T8992	case	's,Student
R5674	T8995	T8993	compound	T,test
R5675	T8996	T8993	punct	-,test
R5676	T8997	T8998	aux	to,judge
R5677	T8998	T8993	advcl	judge,test
R5678	T8999	T9000	mark	if,have
R5679	T9000	T8998	ccomp	have,judge
R568	T1409	T1410	det	a,fashion
R5680	T9001	T9000	nsubj	samples,have
R5681	T9002	T9001	prep	of,samples
R5682	T9003	T9004	amod	different,groups
R5683	T9004	T9002	pobj	groups,of
R5684	T9005	T9006	det	a,value
R5685	T9006	T9000	dobj	value,have
R5686	T9007	T9006	amod	different,value
R5687	T9008	T9006	amod	mean,value
R5688	T9009	T9000	cc	and,have
R5689	T9010	T9000	conj	are,have
R569	T1410	T1408	pobj	fashion,in
R5690	T9011	T9010	attr	parts,are
R5691	T9012	T9010	prep	from,are
R5692	T9013	T9014	amod	different,distributions
R5693	T9014	T9012	pobj	distributions,from
R5694	T9015	T9014	amod	normal,distributions
R5695	T9016	T8960	punct	.,performed
R5696	T9018	T9019	det	The,values
R5697	T9019	T9022	nsubjpass	values,indicated
R5698	T9020	T9019	amod	exact,values
R5699	T9021	T9019	compound	probability,values
R57	T271	T266	aux	has,identified
R570	T1411	T1412	npadvmod	Ca2+,dependent
R5700	T9023	T9019	cc	and,values
R5701	T9024	T9025	det	the,significance
R5702	T9025	T9019	conj	significance,values
R5703	T9026	T9025	prep	of,significance
R5704	T9027	T9028	det	an,analysis
R5705	T9028	T9026	pobj	analysis,of
R5706	T9029	T9022	auxpass	are,indicated
R5707	T9030	T9031	advmod	when,described
R5708	T9031	T9022	advcl	described,indicated
R5709	T9032	T9033	det	the,experiments
R571	T1412	T1410	amod	dependent,fashion
R5710	T9033	T9031	nsubjpass	experiments,described
R5711	T9034	T9031	auxpass	are,described
R5712	T9035	T9022	punct	.,indicated
R5713	T9090	T9089	prep	of,Expression
R5714	T9091	T9092	compound	annexin,A7
R5715	T9092	T9090	pobj	A7,of
R5716	T9093	T9089	prep	in,Expression
R5717	T9094	T9095	amod	human,cells
R5718	T9095	T9093	pobj	cells,in
R5719	T9096	T9095	amod	red,cells
R572	T1413	T1412	punct	-,dependent
R5720	T9097	T9095	compound	blood,cells
R5721	T9098	T9099	punct	(,1
R5722	T9099	T9095	parataxis	1,cells
R5723	T9100	T9099	punct	),1
R5724	T9101	T9095	cc	and,cells
R5725	T9102	T9103	amod	human,platelets
R5726	T9103	T9095	conj	platelets,cells
R5727	T9104	T9105	punct	(,2
R5728	T9105	T9103	parataxis	2,platelets
R5729	T9106	T9105	punct	),2
R573	T1414	T1405	cc	and,translocates
R5730	T9107	T9089	punct	.,Expression
R5731	T9109	T9110	compound	Protein,homogenates
R5732	T9110	T9111	nsubjpass	homogenates,separated
R5733	T9112	T9111	auxpass	were,separated
R5734	T9113	T9111	prep	by,separated
R5735	T9114	T9115	compound	SDS,PAGE
R5736	T9115	T9113	pobj	PAGE,by
R5737	T9116	T9115	punct	-,PAGE
R5738	T9117	T9118	punct	(,acrylamide
R5739	T9118	T9111	parataxis	acrylamide,separated
R574	T1415	T1416	punct	", ",redistributes
R5740	T9119	T9120	nummod	12,%
R5741	T9120	T9118	compound	%,acrylamide
R5742	T9121	T9118	punct	),acrylamide
R5743	T9122	T9111	punct	.,separated
R5744	T9124	T9125	det	The,blot
R5745	T9125	T9128	nsubjpass	blot,probed
R5746	T9126	T9125	amod	resulting,blot
R5747	T9127	T9125	amod	western,blot
R5748	T9128	T9130	ccomp	probed,was
R5749	T9129	T9128	auxpass	was,probed
R575	T1416	T1405	conj	redistributes,translocates
R5750	T9131	T9128	prep	with,probed
R5751	T9132	T9131	pobj	mAb,with
R5752	T9133	T9132	nummod	203–217,mAb
R5753	T9134	T9130	punct	", ",was
R5754	T9135	T9130	nsubj	visualization,was
R5755	T9136	T9130	prep	by,was
R5756	T9137	T9138	det	a,antibody
R5757	T9138	T9136	pobj	antibody,by
R5758	T9139	T9138	amod	secondary,antibody
R5759	T9140	T9141	npadvmod	peroxidase,coupled
R576	T1417	T1418	advmod	when,rise
R5760	T9141	T9138	amod	coupled,antibody
R5761	T9142	T9138	acl	followed,antibody
R5762	T9143	T9142	agent	by,followed
R5763	T9144	T9143	pobj	ECL,by
R5764	T9145	T9130	punct	.,was
R5765	T9147	T9148	det	Both,isoforms
R5766	T9148	T9149	nsubjpass	isoforms,detected
R5767	T9149	T9151	ccomp	detected,is
R5768	T9150	T9149	auxpass	are,detected
R5769	T9152	T9149	prep	in,detected
R577	T1418	T1416	advcl	rise,redistributes
R5770	T9153	T9154	amod	red,cells
R5771	T9154	T9152	pobj	cells,in
R5772	T9155	T9154	compound	blood,cells
R5773	T9156	T9151	punct	", ",is
R5774	T9157	T9151	prep	in,is
R5775	T9158	T9157	pobj	platelets,in
R5776	T9159	T9160	advmod	only,isoform
R5777	T9160	T9151	nsubj	isoform,is
R5778	T9161	T9160	det	the,isoform
R5779	T9162	T9160	amod	small,isoform
R578	T1419	T1420	amod	intracellular,levels
R5780	T9163	T9151	acomp	present,is
R5781	T9164	T9151	punct	.,is
R5782	T9241	T9240	prep	of,Enrichment
R5783	T9242	T9243	compound	annexin,A7
R5784	T9243	T9241	pobj	A7,of
R5785	T9244	T9243	cc	and,A7
R5786	T9245	T9243	conj	sorcin,A7
R5787	T9246	T9240	prep	in,Enrichment
R5788	T9247	T9246	pobj	exovesicles,in
R5789	T9248	T9247	acl	derived,exovesicles
R579	T1420	T1418	nsubj	levels,rise
R5790	T9249	T9248	prep	from,derived
R5791	T9250	T9251	amod	red,cells
R5792	T9251	T9249	pobj	cells,from
R5793	T9252	T9251	compound	blood,cells
R5794	T9253	T9240	punct	.,Enrichment
R5795	T9255	T9256	nsubjpass	Vesicles,generated
R5796	T9257	T9255	prep	from,Vesicles
R5797	T9258	T9259	amod	wild,type
R5798	T9259	T9257	pobj	type,from
R5799	T9260	T9259	punct	(,type
R58	T272	T266	auxpass	been,identified
R580	T1421	T1420	compound	Ca2+,levels
R5800	T9261	T9259	appos	wt,type
R5801	T9262	T9259	punct	),type
R5802	T9263	T9259	cc	and,type
R5803	T9264	T9265	nmod	anxA7,mutant
R5804	T9265	T9259	conj	mutant,type
R5805	T9266	T9264	punct	-,anxA7
R5806	T9267	T9264	punct	/,anxA7
R5807	T9268	T9264	punct	-,anxA7
R5808	T9269	T9265	punct	(,mutant
R5809	T9270	T9265	appos	ko,mutant
R581	T1422	T1416	punct	", ",redistributes
R5810	T9271	T9256	punct	),generated
R5811	T9272	T9256	auxpass	were,generated
R5812	T9273	T9256	prep	with,generated
R5813	T9274	T9275	compound	Ca2+,ionophore
R5814	T9275	T9277	compound	ionophore,treatment
R5815	T9276	T9275	punct	/,ionophore
R5816	T9277	T9273	pobj	treatment,with
R5817	T9278	T9256	punct	", ",generated
R5818	T9279	T9256	conj	isolated,generated
R5819	T9280	T9279	prep	by,isolated
R582	T1423	T1416	advmod	sequentially,redistributes
R5820	T9281	T9282	amod	differential,centrifugation
R5821	T9282	T9280	pobj	centrifugation,by
R5822	T9283	T9279	cc	and,isolated
R5823	T9284	T9279	conj	analysed,isolated
R5824	T9285	T9284	prep	by,analysed
R5825	T9286	T9285	pobj	immunoblotting,by
R5826	T9287	T9284	prep	with,analysed
R5827	T9288	T9289	nmod	mAb,203
R5828	T9289	T9287	pobj	203,with
R5829	T9290	T9291	punct	–,217
R583	T1424	T1416	prep	to,redistributes
R5830	T9291	T9289	prep	217,203
R5831	T9292	T9289	cc	and,203
R5832	T9293	T9294	det	a,antibody
R5833	T9294	T9289	conj	antibody,203
R5834	T9295	T9294	nmod	sorcin,antibody
R5835	T9296	T9294	amod	polyclonal,antibody
R5836	T9297	T9256	punct	.,generated
R5837	T9299	T9300	prep	In,are
R5838	T9301	T9299	amod	general,In
R5839	T9302	T9300	punct	", ",are
R584	T1425	T1426	det	the,plasma
R5840	T9303	T9304	compound	annexin,A7
R5841	T9304	T9300	nsubj	A7,are
R5842	T9305	T9304	cc	and,A7
R5843	T9306	T9304	conj	sorcin,A7
R5844	T9307	T9308	advmod	more,abundant
R5845	T9308	T9300	acomp	abundant,are
R5846	T9309	T9300	prep	in,are
R5847	T9310	T9309	pobj	nanovesicles,in
R5848	T9311	T9300	punct	.,are
R5849	T9313	T9314	det	The,isoform
R585	T1426	T1424	pobj	plasma,to
R5850	T9314	T9317	nsubjpass	isoform,observed
R5851	T9315	T9316	nummod	51,kDa
R5852	T9316	T9314	compound	kDa,isoform
R5853	T9318	T9314	prep	of,isoform
R5854	T9319	T9320	compound	annexin,A7
R5855	T9320	T9318	pobj	A7,of
R5856	T9321	T9322	punct	(,arrows
R5857	T9322	T9314	parataxis	arrows,isoform
R5858	T9323	T9322	punct	),arrows
R5859	T9324	T9317	auxpass	is,observed
R586	T1427	T1426	cc	and,plasma
R5860	T9325	T9317	advmod	only,observed
R5861	T9326	T9317	prep	in,observed
R5862	T9327	T9326	pobj	nanovesicles,in
R5863	T9328	T9317	punct	.,observed
R5864	T9330	T9331	nsubjpass	Samples,normalized
R5865	T9332	T9330	prep	of,Samples
R5866	T9333	T9334	det	both,types
R5867	T9334	T9332	pobj	types,of
R5868	T9335	T9334	compound	vesicles,types
R5869	T9336	T9331	auxpass	were,normalized
R587	T1428	T1429	det	the,membrane
R5870	T9337	T9331	prep	according,normalized
R5871	T9338	T9337	prep	to,according
R5872	T9339	T9340	poss	their,activity
R5873	T9340	T9338	pobj	activity,to
R5874	T9341	T9342	compound	acetylcholine,esterase
R5875	T9342	T9340	compound	esterase,activity
R5876	T9343	T9331	punct	.,normalized
R5877	T9345	T9346	amod	Human,cells
R5878	T9346	T9349	nsubjpass	cells,used
R5879	T9347	T9346	amod	red,cells
R588	T1429	T1426	conj	membrane,plasma
R5880	T9348	T9346	compound	blood,cells
R5881	T9350	T9351	punct	(,co
R5882	T9351	T9346	parataxis	co,cells
R5883	T9352	T9351	punct	),co
R5884	T9353	T9349	auxpass	were,used
R5885	T9354	T9349	prep	for,used
R5886	T9355	T9354	pobj	control,for
R5887	T9356	T9349	cc	and,used
R5888	T9357	T9349	conj	normalized,used
R5889	T9358	T9357	advmod	independently,normalized
R589	T1430	T1429	amod	nuclear,membrane
R5890	T9359	T9349	punct	.,used
R5891	T9398	T9397	prep	of,Association
R5892	T9399	T9400	compound	annexin,A7
R5893	T9400	T9398	pobj	A7,of
R5894	T9401	T9400	cc	and,A7
R5895	T9402	T9400	conj	sorcin,A7
R5896	T9403	T9397	prep	with,Association
R5897	T9404	T9405	amod	distinct,fractions
R5898	T9405	T9403	pobj	fractions,with
R5899	T9406	T9407	amod	red,cell
R59	T273	T266	prep	in,identified
R590	T1431	T1432	advmod	as,as
R5900	T9407	T9405	compound	cell,fractions
R5901	T9408	T9407	compound	blood,cell
R5902	T9409	T9410	compound	plasma,membrane
R5903	T9410	T9405	compound	membrane,fractions
R5904	T9411	T9397	punct	.,Association
R5905	T9413	T9414	amod	Lysed,cells
R5906	T9414	T9418	nsubjpass	cells,added
R5907	T9415	T9414	amod	human,cells
R5908	T9416	T9414	amod	red,cells
R5909	T9417	T9414	compound	blood,cells
R591	T1432	T1424	cc	as,to
R5910	T9419	T9418	auxpass	were,added
R5911	T9420	T9418	prep	to,added
R5912	T9421	T9422	det	a,gradient
R5913	T9422	T9420	pobj	gradient,to
R5914	T9423	T9424	npadvmod	self,forming
R5915	T9424	T9422	amod	forming,gradient
R5916	T9425	T9422	compound	iodixanol,gradient
R5917	T9426	T9422	compound	density,gradient
R5918	T9427	T9418	punct	.,added
R5919	T9429	T9430	det	The,density
R592	T1433	T1432	advmod	well,as
R5920	T9430	T9431	nsubj	density,increases
R5921	T9432	T9430	prep	of,density
R5922	T9433	T9434	det	the,gradient
R5923	T9434	T9432	pobj	gradient,of
R5924	T9435	T9431	prep	from,increases
R5925	T9436	T9435	pobj	left,from
R5926	T9437	T9438	punct	(,g
R5927	T9438	T9436	parataxis	g,left
R5928	T9439	T9438	nummod	1.06,g
R5929	T9440	T9441	punct	/,ml
R593	T1434	T1424	conj	to,to
R5930	T9441	T9438	prep	ml,g
R5931	T9442	T9438	punct	),g
R5932	T9443	T9435	prep	to,from
R5933	T9444	T9443	pobj	right,to
R5934	T9445	T9446	punct	(,g
R5935	T9446	T9444	parataxis	g,right
R5936	T9447	T9446	nummod	1.20,g
R5937	T9448	T9449	punct	/,ml
R5938	T9449	T9446	prep	ml,g
R5939	T9450	T9446	punct	),g
R594	T1435	T1436	amod	intracellular,vesicles
R5940	T9451	T9431	punct	.,increases
R5941	T9453	T9454	nsubjpass	Fractions,analysed
R5942	T9455	T9454	auxpass	were,analysed
R5943	T9456	T9454	prep	by,analysed
R5944	T9457	T9456	pobj	immunoblotting,by
R5945	T9458	T9454	advcl	using,analysed
R5946	T9459	T9460	nmod	mAb,203
R5947	T9460	T9458	dobj	203,using
R5948	T9461	T9462	punct	–,217
R5949	T9462	T9460	prep	217,203
R595	T1436	T1434	pobj	vesicles,to
R5950	T9463	T9460	cc	and,203
R5951	T9464	T9465	det	the,antibody
R5952	T9465	T9460	conj	antibody,203
R5953	T9466	T9465	nmod	sorcin,antibody
R5954	T9467	T9465	amod	polyclonal,antibody
R5955	T9468	T9454	punct	.,analysed
R5956	T9489	T9490	amod	Dark,field
R5957	T9490	T9491	compound	field,microscopy
R5958	T9492	T9491	prep	of,microscopy
R5959	T9493	T9494	amod	red,cells
R596	T1437	T1405	punct	.,translocates
R5960	T9494	T9492	pobj	cells,of
R5961	T9495	T9494	compound	blood,cells
R5962	T9496	T9494	prep	from,cells
R5963	T9497	T9498	nmod	anxA7,mutant
R5964	T9498	T9502	nmod	mutant,mice
R5965	T9499	T9497	punct	-,anxA7
R5966	T9500	T9497	punct	/,anxA7
R5967	T9501	T9497	punct	-,anxA7
R5968	T9502	T9496	pobj	mice,from
R5969	T9503	T9504	punct	(,B
R597	T1439	T1440	advmod	Furthermore,associates
R5970	T9504	T9498	parataxis	B,mutant
R5971	T9505	T9504	punct	),B
R5972	T9506	T9498	cc	and,mutant
R5973	T9507	T9508	amod	wild,type
R5974	T9508	T9498	conj	type,mutant
R5975	T9509	T9510	punct	(,A
R5976	T9510	T9502	parataxis	A,mice
R5977	T9511	T9510	punct	),A
R5978	T9512	T9491	punct	.,microscopy
R5979	T9514	T9515	det	The,diameters
R598	T1441	T1440	punct	", ",associates
R5980	T9515	T9518	nsubj	diameters,are
R5981	T9516	T9515	amod	mean,diameters
R5982	T9517	T9515	amod	cellular,diameters
R5983	T9519	T9520	nummod	6.0,μm
R5984	T9520	T9518	attr	μm,are
R5985	T9521	T9520	cc	and,μm
R5986	T9522	T9523	nummod	5.7,μm
R5987	T9523	T9520	conj	μm,μm
R5988	T9524	T9518	punct	", ",are
R5989	T9525	T9518	advmod	respectively,are
R599	T1442	T1443	compound	annexin,A7
R5990	T9526	T9518	punct	.,are
R5991	T9528	T9529	det	The,differences
R5992	T9529	T9531	nsubj	differences,are
R5993	T9530	T9529	compound	size,differences
R5994	T9532	T9533	advmod	statistically,significant
R5995	T9533	T9531	acomp	significant,are
R5996	T9534	T9535	punct	(,40
R5997	T9535	T9531	parataxis	40,are
R5998	T9536	T9537	nsubj	p,0.015
R5999	T9537	T9535	ccomp	0.015,40
R6	T213	T214	compound	annexin,A7
R60	T274	T273	pobj	erythrocytes,in
R600	T1443	T1440	nsubj	A7,associates
R6000	T9538	T9537	punct	=,0.015
R6001	T9539	T9535	punct	;,40
R6002	T9540	T9535	nsubj	n,40
R6003	T9541	T9535	punct	=,40
R6004	T9542	T9535	punct	),40
R6005	T9543	T9531	punct	.,are
R6006	T9545	T9546	dep	Bar,μm
R6007	T9547	T9546	punct	", ",μm
R6008	T9548	T9546	nummod	3,μm
R6009	T9549	T9546	punct	.,μm
R601	T1444	T1440	prep	with,associates
R6014	T9617	T9618	amod	Osmotic,resistance
R6015	T9618	T9619	compound	resistance,curves
R6016	T9620	T9619	prep	of,curves
R6017	T9621	T9622	amod	red,cells
R6018	T9622	T9620	pobj	cells,of
R6019	T9623	T9622	compound	blood,cells
R602	T1445	T1446	compound	lipid,rafts
R6020	T9624	T9619	prep	from,curves
R6021	T9625	T9626	amod	wild,type
R6022	T9626	T9627	nmod	type,mice
R6023	T9627	T9624	pobj	mice,from
R6024	T9628	T9626	cc	and,type
R6025	T9629	T9626	conj	anxA7,type
R6026	T9630	T9629	punct	-,anxA7
R6027	T9631	T9629	punct	/,anxA7
R6028	T9632	T9629	punct	-,anxA7
R6029	T9633	T9627	amod	mutant,mice
R603	T1446	T1444	pobj	rafts,with
R6030	T9634	T9619	punct	.,curves
R6031	T9636	T9637	det	The,resistance
R6032	T9637	T9639	nsubj	resistance,is
R6033	T9638	T9637	amod	osmotic,resistance
R6034	T9640	T9637	prep	towards,resistance
R6035	T9641	T9640	pobj	changes,towards
R6036	T9642	T9641	prep	in,changes
R6037	T9643	T9644	det	the,strength
R6038	T9644	T9642	pobj	strength,in
R6039	T9645	T9644	amod	extracellular,strength
R604	T1447	T1448	punct	[,7
R6040	T9646	T9644	amod	ionic,strength
R6041	T9647	T9648	det	a,assay
R6042	T9648	T9639	attr	assay,is
R6043	T9649	T9648	amod	convenient,assay
R6044	T9650	T9648	prep	for,assay
R6045	T9651	T9650	pobj	analysis,for
R6046	T9652	T9651	prep	of,analysis
R6047	T9653	T9654	det	the,integrity
R6048	T9654	T9652	pobj	integrity,of
R6049	T9655	T9656	amod	red,cell
R605	T1448	T1440	parataxis	7,associates
R6050	T9656	T9654	compound	cell,integrity
R6051	T9657	T9656	compound	blood,cell
R6052	T9658	T9639	punct	.,is
R6053	T9660	T9661	nsubjpass	It,measured
R6054	T9662	T9661	auxpass	is,measured
R6055	T9663	T9661	prep	as,measured
R6056	T9664	T9665	det	a,concentration
R6057	T9665	T9663	pobj	concentration,as
R6058	T9666	T9667	compound	sodium,chloride
R6059	T9667	T9665	compound	chloride,concentration
R606	T1449	T1448	punct	],7
R6060	T9668	T9669	prep	in,starts
R6061	T9669	T9665	relcl	starts,concentration
R6062	T9670	T9668	pobj	which,in
R6063	T9671	T9672	amod	cellular,lysis
R6064	T9672	T9669	nsubj	lysis,starts
R6065	T9673	T9674	punct	(,resistance
R6066	T9674	T9665	parataxis	resistance,concentration
R6067	T9675	T9674	amod	minimal,resistance
R6068	T9676	T9674	punct	),resistance
R6069	T9677	T9665	prep	up,concentration
R607	T1450	T1440	punct	.,associates
R6070	T9678	T9677	prep	to,up
R6071	T9679	T9680	det	a,lysis
R6072	T9680	T9678	pobj	lysis,to
R6073	T9681	T9680	amod	complete,lysis
R6074	T9682	T9683	punct	(,resistance
R6075	T9683	T9680	parataxis	resistance,lysis
R6076	T9684	T9683	amod	maximal,resistance
R6077	T9685	T9683	punct	),resistance
R6078	T9686	T9680	prep	of,lysis
R6079	T9687	T9688	det	a,cell
R608	T1452	T1453	compound	Lipid,rafts
R6080	T9688	T9686	pobj	cell,of
R6081	T9689	T9688	amod	red,cell
R6082	T9690	T9688	compound	blood,cell
R6083	T9691	T9661	punct	.,measured
R6084	T9693	T9694	det	The,resistance
R6085	T9694	T9696	nsubj	resistance,is
R6086	T9695	T9694	amod	osmotic,resistance
R6087	T9697	T9694	prep	of,resistance
R6088	T9698	T9699	compound	annexin,A7
R6089	T9699	T9700	npadvmod	A7,deficient
R609	T1453	T1454	nsubj	rafts,play
R6090	T9700	T9701	amod	deficient,cells
R6091	T9701	T9697	pobj	cells,of
R6092	T9702	T9701	amod	red,cells
R6093	T9703	T9701	compound	blood,cells
R6094	T9704	T9705	advmod	significantly,increased
R6095	T9705	T9696	acomp	increased,is
R6096	T9706	T9696	prep	compared,is
R6097	T9707	T9706	prep	to,compared
R6098	T9708	T9709	det	the,one
R6099	T9709	T9707	pobj	one,to
R61	T275	T276	advmod	where,proposed
R610	T1455	T1456	det	a,role
R6100	T9710	T9709	prep	of,one
R6101	T9711	T9712	amod	wild,type
R6102	T9712	T9710	pobj	type,of
R6103	T9713	T9714	punct	(,8
R6104	T9714	T9696	parataxis	8,is
R6105	T9715	T9716	nsubj	p,0.00066
R6106	T9716	T9714	ccomp	0.00066,8
R6107	T9717	T9716	punct	=,0.00066
R6108	T9718	T9714	punct	;,8
R6109	T9719	T9714	nsubj	n,8
R611	T1456	T1454	dobj	role,play
R6110	T9720	T9714	punct	=,8
R6111	T9721	T9714	punct	),8
R6112	T9722	T9696	punct	.,is
R6113	T9783	T9784	compound	Platelet,aggregation
R6114	T9784	T9785	compound	aggregation,curves
R6115	T9786	T9785	prep	from,curves
R6116	T9787	T9788	npadvmod	platelet,rich
R6117	T9788	T9789	amod	rich,plasma
R6118	T9789	T9786	pobj	plasma,from
R6119	T9790	T9789	prep	of,plasma
R612	T1457	T1456	amod	key,role
R6120	T9791	T9792	nmod	annexin,A7
R6121	T9792	T9793	nmod	A7,mice
R6122	T9793	T9790	pobj	mice,of
R6123	T9794	T9795	compound	knock,out
R6124	T9795	T9792	appos	out,A7
R6125	T9796	T9795	cc	and,out
R6126	T9797	T9798	amod	wild,type
R6127	T9798	T9795	conj	type,out
R6128	T9799	T9785	punct	.,curves
R6129	T9801	T9802	det	The,values
R613	T1458	T1454	prep	in,play
R6130	T9802	T9804	nsubjpass	values,measured
R6131	T9803	T9802	compound	transmission,values
R6132	T9805	T9804	auxpass	were,measured
R6133	T9806	T9804	prep	with,measured
R6134	T9807	T9808	det	an,photometer
R6135	T9808	T9806	pobj	photometer,with
R6136	T9809	T9808	compound	APACT,photometer
R6137	T9810	T9804	cc	and,measured
R6138	T9811	T9812	nsubjpass	aggregation,initiated
R6139	T9812	T9804	conj	initiated,measured
R614	T1459	T1460	compound	membrane,budding
R6140	T9813	T9812	auxpass	was,initiated
R6141	T9814	T9812	prep	by,initiated
R6142	T9815	T9814	pcomp	adding,by
R6143	T9816	T9815	dobj	ristocetin,adding
R6144	T9817	T9812	punct	.,initiated
R6145	T9819	T9820	det	The,seconds
R6146	T9820	T9823	nsubjpass	seconds,given
R6147	T9821	T9820	amod	first,seconds
R6148	T9822	T9820	nummod	thirty,seconds
R6149	T9824	T9823	auxpass	are,given
R615	T1460	T1458	pobj	budding,in
R6150	T9825	T9823	punct	.,given
R6151	T9827	T9828	amod	Constant,counts
R6152	T9828	T9830	nsubjpass	counts,used
R6153	T9829	T9828	compound	platelet,counts
R6154	T9831	T9830	auxpass	were,used
R6155	T9832	T9830	prep	throughout,used
R6156	T9833	T9834	det	all,experiments
R6157	T9834	T9832	pobj	experiments,throughout
R6158	T9835	T9830	punct	.,used
R6159	T9837	T9838	det	The,data
R616	T1461	T1458	cc	and,in
R6160	T9838	T9841	nsubjpass	data,analysed
R6161	T9839	T9840	compound	aggregation,curve
R6162	T9840	T9838	compound	curve,data
R6163	T9841	T9843	ccomp	analysed,set
R6164	T9842	T9841	auxpass	were,analysed
R6165	T9844	T9841	prep	for,analysed
R6166	T9845	T9846	compound	slope,values
R6167	T9846	T9844	pobj	values,for
R6168	T9847	T9843	punct	", ",set
R6169	T9848	T9849	det	the,amplitude
R617	T1462	T1458	conj	in,in
R6170	T9849	T9843	nsubjpass	amplitude,set
R6171	T9850	T9849	amod	maximal,amplitude
R6172	T9851	T9849	compound	aggregation,amplitude
R6173	T9852	T9849	prep	of,amplitude
R6174	T9853	T9854	det	every,curve
R6175	T9854	T9852	pobj	curve,of
R6176	T9855	T9854	amod	single,curve
R6177	T9856	T9843	auxpass	was,set
R6178	T9857	T9843	prep	to,set
R6179	T9858	T9859	nummod	100,%
R618	T1463	T1464	compound	vesiculation,processes
R6180	T9859	T9857	pobj	%,to
R6181	T9860	T9843	punct	.,set
R6182	T9862	T9863	det	Both,curves
R6183	T9863	T9865	nsubj	curves,differ
R6184	T9864	T9863	amod	murine,curves
R6185	T9865	T9866	ccomp	differ,shows
R6186	T9867	T9865	advmod	significantly,differ
R6187	T9868	T9865	prep	at,differ
R6188	T9869	T9870	det	a,point
R6189	T9870	T9868	pobj	point,at
R619	T1464	T1462	pobj	processes,in
R6190	T9871	T9870	compound	time,point
R6191	T9872	T9870	prep	of,point
R6192	T9873	T9874	nummod	seven,seconds
R6193	T9874	T9872	pobj	seconds,of
R6194	T9875	T9874	prep	after,seconds
R6195	T9876	T9877	compound	platelet,initiation
R6196	T9877	T9875	pobj	initiation,after
R6197	T9878	T9866	punct	", ",shows
R6198	T9879	T9880	det	the,mutant
R6199	T9880	T9866	nsubj	mutant,shows
R62	T276	T274	relcl	proposed,erythrocytes
R620	T1465	T1466	amod	such,as
R6200	T9881	T9882	det	a,velocity
R6201	T9882	T9866	dobj	velocity,shows
R6202	T9883	T9884	advmod	slightly,lower
R6203	T9884	T9882	amod	lower,velocity
R6204	T9885	T9882	compound	aggregation,velocity
R6205	T9886	T9887	punct	(,transmission
R6206	T9887	T9882	parataxis	transmission,velocity
R6207	T9888	T9889	nsubj	p,0.0287
R6208	T9889	T9887	ccomp	0.0287,transmission
R6209	T9890	T9889	punct	=,0.0287
R621	T1466	T1464	prep	as,processes
R6210	T9891	T9887	punct	", ",transmission
R6211	T9892	T9893	compound	n,out
R6212	T9893	T9895	nsubj	out,16
R6213	T9894	T9893	compound	knock,out
R6214	T9895	T9887	ccomp	16,transmission
R6215	T9896	T9895	punct	=,16
R6216	T9897	T9887	punct	", ",transmission
R6217	T9898	T9899	nmod	n,type
R6218	T9899	T9901	nsubj	type,15
R6219	T9900	T9899	amod	wild,type
R622	T1467	T1468	nmod	endo,exocytosis
R6220	T9901	T9887	ccomp	15,transmission
R6221	T9902	T9901	punct	=,15
R6222	T9903	T9887	punct	", ",transmission
R6223	T9904	T9905	amod	mean,out
R6224	T9905	T9907	nsubj	out,transmission
R6225	T9906	T9905	compound	knock,out
R6226	T9907	T9887	ccomp	transmission,transmission
R6227	T9908	T9907	punct	=,transmission
R6228	T9909	T9910	nummod	67.7,%
R6229	T9910	T9907	compound	%,transmission
R623	T1468	T1466	pobj	exocytosis,as
R6230	T9911	T9887	punct	", ",transmission
R6231	T9912	T9913	amod	mean,type
R6232	T9913	T9915	nsubj	type,transmission
R6233	T9914	T9913	amod	wild,type
R6234	T9915	T9887	ccomp	transmission,transmission
R6235	T9916	T9915	punct	=,transmission
R6236	T9917	T9918	nummod	77.1,%
R6237	T9918	T9915	compound	%,transmission
R6238	T9919	T9887	punct	", ",transmission
R6239	T9920	T9921	amod	standard,error
R624	T1469	T1468	punct	-,exocytosis
R6240	T9921	T9922	compound	error,out
R6241	T9922	T9924	nsubj	out,transmission
R6242	T9923	T9922	compound	knock,out
R6243	T9924	T9887	ccomp	transmission,transmission
R6244	T9925	T9924	punct	=,transmission
R6245	T9926	T9927	nummod	8.3,%
R6246	T9927	T9924	compound	%,transmission
R6247	T9928	T9887	punct	", ",transmission
R6248	T9929	T9930	amod	standard,error
R6249	T9930	T9931	nmod	error,type
R625	T1470	T1468	cc	and,exocytosis
R6250	T9931	T9887	nsubj	type,transmission
R6251	T9932	T9931	amod	wild,type
R6252	T9933	T9887	punct	=,transmission
R6253	T9934	T9935	nummod	13.8,%
R6254	T9935	T9887	compound	%,transmission
R6255	T9936	T9887	punct	),transmission
R6256	T9937	T9866	punct	.,shows
R6257	T9939	T9940	amod	Murine,platelets
R6258	T9940	T9941	nsubj	platelets,react
R6259	T9942	T9941	advmod	immediately,react
R626	T1471	T1472	punct	[,8
R6260	T9943	T9941	prep	to,react
R6261	T9944	T9945	det	the,chemical
R6262	T9945	T9943	pobj	chemical,to
R6263	T9946	T9945	amod	initiating,chemical
R6264	T9947	T9941	cc	and,react
R6265	T9948	T9941	conj	show,react
R6266	T9949	T9950	det	no,phase
R6267	T9950	T9948	dobj	phase,show
R6268	T9951	T9950	compound	lag,phase
R6269	T9952	T9948	prep	like,show
R627	T1472	T1454	parataxis	8,play
R6270	T9953	T9954	det	the,platelets
R6271	T9954	T9952	pobj	platelets,like
R6272	T9955	T9954	amod	normal,platelets
R6273	T9956	T9954	amod	human,platelets
R6274	T9957	T9941	punct	.,react
R6275	T9959	T9960	prep	For,shown
R6276	T9961	T9959	pobj	comparison,For
R6277	T9962	T9961	prep	of,comparison
R6278	T9963	T9964	det	the,range
R6279	T9964	T9962	pobj	range,of
R628	T1473	T1474	punct	-,10
R6280	T9965	T9964	prep	of,range
R6281	T9966	T9967	det	the,impairment
R6282	T9967	T9965	pobj	impairment,of
R6283	T9968	T9967	compound	platelet,impairment
R6284	T9969	T9970	advmod	slightly,affected
R6285	T9970	T9971	amod	affected,platelets
R6286	T9971	T9960	nsubjpass	platelets,shown
R6287	T9972	T9971	amod	human,platelets
R6288	T9973	T9960	auxpass	are,shown
R6289	T9974	T9975	advmod	as,well
R629	T1474	T1472	prep	10,8
R6290	T9975	T9960	advmod	well,shown
R6291	T9976	T9977	punct	(,syndrome
R6292	T9977	T9960	parataxis	syndrome,shown
R6293	T9978	T9979	compound	von,Willebrand
R6294	T9979	T9977	compound	Willebrand,syndrome
R6295	T9980	T9977	punct	),syndrome
R6296	T9981	T9960	punct	.,shown
R63	T277	T276	nsubjpass	it,proposed
R630	T1475	T1472	punct	],8
R631	T1476	T1454	punct	.,play
R632	T1478	T1479	nummod	Two,partners
R633	T1479	T1481	nsubjpass	partners,identified
R634	T1480	T1479	amod	binding,partners
R635	T1482	T1479	prep	of,partners
R636	T1483	T1484	compound	annexin,A7
R637	T1484	T1482	pobj	A7,of
R638	T1485	T1481	aux	have,identified
R639	T1486	T1481	auxpass	been,identified
R64	T278	T276	auxpass	was,proposed
R640	T1487	T1481	punct	", ",identified
R641	T1488	T1481	dobj	sorcin,identified
R642	T1489	T1488	cc	and,sorcin
R643	T1490	T1488	conj	galectin,sorcin
R644	T1491	T1490	punct	-,galectin
R645	T1492	T1490	nummod	3,galectin
R646	T1493	T1494	punct	[,11
R647	T1494	T1481	parataxis	11,identified
R648	T1495	T1496	punct	-,13
R649	T1496	T1494	prep	13,11
R65	T279	T280	aux	to,be
R650	T1497	T1494	punct	],11
R651	T1498	T1481	punct	.,identified
R652	T1500	T1501	nsubj	Sorcin,is
R653	T1502	T1503	det	a,protein
R654	T1503	T1501	attr	protein,is
R655	T1504	T1505	npadvmod	Ca2+,binding
R656	T1505	T1503	amod	binding,protein
R657	T1506	T1505	punct	-,binding
R658	T1507	T1501	cc	and,is
R659	T1508	T1501	conj	belongs,is
R66	T280	T276	xcomp	be,proposed
R660	T1509	T1508	prep	to,belongs
R661	T1510	T1511	det	the,family
R662	T1511	T1509	pobj	family,to
R663	T1512	T1513	compound	penta,protein
R664	T1513	T1511	compound	protein,family
R665	T1514	T1515	compound	EF,hand
R666	T1515	T1513	compound	hand,protein
R667	T1516	T1515	punct	-,hand
R668	T1517	T1518	punct	[,14
R669	T1518	T1508	parataxis	14,belongs
R67	T281	T282	det	a,component
R670	T1519	T1518	punct	],14
R671	T1520	T1501	punct	.,is
R672	T1522	T1523	prep	Like,binds
R673	T1524	T1525	compound	annexin,A7
R674	T1525	T1522	pobj	A7,Like
R675	T1526	T1523	nsubj	it,binds
R676	T1527	T1523	prep	to,binds
R677	T1528	T1527	pobj	membranes,to
R678	T1529	T1523	prep	in,binds
R679	T1530	T1531	det	a,manner
R68	T282	T280	attr	component,be
R680	T1531	T1529	pobj	manner,in
R681	T1532	T1533	npadvmod	Ca2+,dependent
R682	T1533	T1531	amod	dependent,manner
R683	T1534	T1533	punct	-,dependent
R684	T1535	T1523	punct	.,binds
R685	T1537	T1538	nsubjpass	Sorcin,described
R686	T1539	T1538	advmod	also,described
R687	T1540	T1538	aux	has,described
R688	T1541	T1538	auxpass	been,described
R689	T1542	T1538	prep	as,described
R69	T283	T282	amod	key,component
R690	T1543	T1544	compound	interaction,partner
R691	T1544	T1542	pobj	partner,as
R692	T1545	T1544	prep	of,partner
R693	T1546	T1547	det	the,receptor
R694	T1547	T1545	pobj	receptor,of
R695	T1548	T1547	compound	ryanodine,receptor
R696	T1549	T1538	punct	", ",described
R697	T1550	T1538	cc	and,described
R698	T1551	T1538	conj	appears,described
R699	T1552	T1553	aux	to,modulate
R7	T214	T212	pobj	A7,of
R70	T284	T282	prep	in,component
R700	T1553	T1551	xcomp	modulate,appears
R701	T1554	T1555	poss	its,function
R702	T1555	T1553	dobj	function,modulate
R703	T1556	T1557	punct	[,15
R704	T1557	T1551	parataxis	15,appears
R705	T1558	T1557	punct	],15
R706	T1559	T1538	punct	.,described
R707	T1561	T1562	det	The,influence
R708	T1562	T1563	nsubj	influence,is
R709	T1564	T1562	prep	of,influence
R71	T285	T286	det	the,process
R710	T1565	T1566	det	the,interaction
R711	T1566	T1564	pobj	interaction,of
R712	T1567	T1568	compound	sorcin,annexin
R713	T1568	T1570	compound	annexin,A7
R714	T1569	T1568	punct	/,annexin
R715	T1570	T1566	compound	A7,interaction
R716	T1571	T1562	prep	on,influence
R717	T1572	T1573	det	the,receptor
R718	T1573	T1571	pobj	receptor,on
R719	T1574	T1573	compound	ryanodine,receptor
R72	T286	T284	pobj	process,in
R720	T1575	T1563	acomp	unknown,is
R721	T1576	T1563	punct	.,is
R722	T1578	T1579	nsubjpass	Sorcin,coexpressed
R723	T1580	T1578	cc	and,Sorcin
R724	T1581	T1582	compound	annexin,A7
R725	T1582	T1578	conj	A7,Sorcin
R726	T1583	T1579	auxpass	are,coexpressed
R727	T1584	T1579	prep	in,coexpressed
R728	T1585	T1586	det	all,tissues
R729	T1586	T1584	pobj	tissues,in
R73	T287	T286	prep	of,process
R730	T1587	T1586	acl	examined,tissues
R731	T1588	T1589	advmod	so,far
R732	T1589	T1587	advmod	far,examined
R733	T1590	T1591	punct	[,11
R734	T1591	T1579	parataxis	11,coexpressed
R735	T1592	T1591	punct	],11
R736	T1593	T1579	punct	.,coexpressed
R737	T1595	T1596	det	The,binding
R738	T1596	T1597	nsubj	binding,is
R739	T1598	T1596	prep	of,binding
R74	T288	T289	det	the,release
R740	T1599	T1600	compound	annexin,A7
R741	T1600	T1598	pobj	A7,of
R742	T1601	T1600	cc	and,A7
R743	T1602	T1600	conj	sorcin,A7
R744	T1603	T1604	npadvmod	Ca2+,dependent
R745	T1604	T1597	acomp	dependent,is
R746	T1605	T1604	punct	-,dependent
R747	T1606	T1597	cc	and,is
R748	T1607	T1597	conj	occurs,is
R749	T1608	T1607	prep	at,occurs
R75	T289	T287	pobj	release,of
R750	T1609	T1610	amod	micromolar,Ca2+
R751	T1610	T1611	compound	Ca2+,concentrations
R752	T1611	T1608	pobj	concentrations,at
R753	T1612	T1597	punct	.,is
R754	T1614	T1615	det	The,sites
R755	T1615	T1617	nsubjpass	sites,localised
R756	T1616	T1615	amod	binding,sites
R757	T1618	T1617	aux	have,localised
R758	T1619	T1617	auxpass	been,localised
R759	T1620	T1617	prep	to,localised
R76	T290	T291	npadvmod	Ca2+,dependent
R760	T1621	T1622	det	the,motifs
R761	T1622	T1620	pobj	motifs,to
R762	T1623	T1624	npadvmod	amino,terminal
R763	T1624	T1622	amod	terminal,motifs
R764	T1625	T1622	nmod	GGYY,motifs
R765	T1626	T1625	cc	and,GGYY
R766	T1627	T1625	conj	GYGG,GGYY
R767	T1628	T1622	prep	in,motifs
R768	T1629	T1628	pobj	sorcin,in
R769	T1630	T1620	cc	and,to
R77	T291	T289	amod	dependent,release
R770	T1631	T1620	conj	to,to
R771	T1632	T1633	det	the,motif
R772	T1633	T1631	pobj	motif,to
R773	T1634	T1633	compound	GYPP,motif
R774	T1635	T1633	prep	in,motif
R775	T1636	T1637	det	the,terminus
R776	T1637	T1635	pobj	terminus,in
R777	T1638	T1637	compound	amino,terminus
R778	T1639	T1637	prep	of,terminus
R779	T1640	T1641	compound	annexin,A7
R78	T292	T291	punct	-,dependent
R780	T1641	T1639	pobj	A7,of
R781	T1642	T1617	punct	.,localised
R782	T1644	T1645	det	The,proteins
R783	T1645	T1646	nsubj	proteins,bind
R784	T1647	T1646	prep	to,bind
R785	T1648	T1649	det	each,other
R786	T1649	T1647	pobj	other,to
R787	T1650	T1646	prep	with,bind
R788	T1651	T1652	det	a,stoichiometry
R789	T1652	T1650	pobj	stoichiometry,with
R79	T293	T289	compound	vesicle,release
R790	T1653	T1652	prep	of,stoichiometry
R791	T1654	T1655	nummod	two,molecules
R792	T1655	T1653	pobj	molecules,of
R793	T1656	T1655	compound	sorcin,molecules
R794	T1657	T1655	prep	per,molecules
R795	T1658	T1659	compound	annexin,A7
R796	T1659	T1660	compound	A7,molecule
R797	T1660	T1657	pobj	molecule,per
R798	T1661	T1662	punct	[,12
R799	T1662	T1646	parataxis	12,bind
R8	T215	T205	prep	in,Function
R80	T294	T286	punct	", ",process
R800	T1663	T1662	punct	],12
R801	T1664	T1646	punct	.,bind
R802	T1666	T1667	nsubj	Galectin,is
R803	T1668	T1666	punct	-,Galectin
R804	T1669	T1666	nummod	3,Galectin
R805	T1670	T1671	det	a,protein
R806	T1671	T1667	attr	protein,is
R807	T1672	T1671	amod	multifunctional,protein
R808	T1673	T1671	amod	oncogenic,protein
R809	T1674	T1671	prep	with,protein
R81	T295	T296	det	a,process
R810	T1675	T1676	det	an,activity
R811	T1676	T1674	pobj	activity,with
R812	T1677	T1676	amod	anti-apoptotic,activity
R813	T1678	T1671	acl	found,protein
R814	T1679	T1678	prep	in,found
R815	T1680	T1681	det	the,space
R816	T1681	T1679	pobj	space,in
R817	T1682	T1681	amod	extracellular,space
R818	T1683	T1681	punct	", ",space
R819	T1684	T1681	prep	in,space
R82	T296	T286	appos	process,process
R820	T1685	T1686	det	the,nucleus
R821	T1686	T1684	pobj	nucleus,in
R822	T1687	T1686	cc	and,nucleus
R823	T1688	T1686	conj	cytoplasm,nucleus
R824	T1689	T1679	cc	and,in
R825	T1690	T1679	conj	in,in
R826	T1691	T1690	pobj	mitochondria,in
R827	T1692	T1667	punct	.,is
R828	T1694	T1695	amod	Cytoplasmic,galectin
R829	T1695	T1696	nsubj	galectin,correlates
R83	T297	T298	prep	with,protect
R830	T1697	T1695	punct	-,galectin
R831	T1698	T1695	nummod	3,galectin
R832	T1699	T1696	prep	with,correlates
R833	T1700	T1701	compound	tumor,progression
R834	T1701	T1699	pobj	progression,with
R835	T1702	T1696	cc	and,correlates
R836	T1703	T1696	conj	protects,correlates
R837	T1704	T1705	amod	mitochondrial,integrity
R838	T1705	T1703	dobj	integrity,protects
R839	T1706	T1696	punct	.,correlates
R84	T298	T296	relcl	protect,process
R840	T1708	T1709	compound	Down,regulation
R841	T1709	T1710	nsubj	regulation,prevents
R842	T1711	T1709	prep	of,regulation
R843	T1712	T1713	compound	annexin,A7
R844	T1713	T1711	pobj	A7,of
R845	T1714	T1715	compound	galectin,3
R846	T1715	T1717	compound	3,translocation
R847	T1716	T1715	punct	-,3
R848	T1717	T1710	dobj	translocation,prevents
R849	T1718	T1717	prep	to,translocation
R85	T299	T297	pobj	which,with
R850	T1719	T1720	det	the,membrane
R851	T1720	T1718	pobj	membrane,to
R852	T1721	T1720	amod	perinuclear,membrane
R853	T1722	T1710	cc	and,prevents
R854	T1723	T1710	conj	increases,prevents
R855	T1724	T1725	compound	galectin,3
R856	T1725	T1727	compound	3,secretion
R857	T1726	T1725	punct	-,3
R858	T1727	T1723	dobj	secretion,increases
R859	T1728	T1710	punct	.,prevents
R86	T300	T301	amod	red,cells
R860	T1730	T1731	prep	For,proposed
R861	T1732	T1733	compound	annexin,A7
R862	T1733	T1730	pobj	A7,For
R863	T1734	T1735	det	a,role
R864	T1735	T1731	nsubjpass	role,proposed
R865	T1736	T1735	prep	for,role
R866	T1737	T1738	compound	galectin,3
R867	T1738	T1740	compound	3,trafficking
R868	T1739	T1738	punct	-,3
R869	T1740	T1736	pobj	trafficking,for
R87	T301	T298	nsubj	cells,protect
R870	T1741	T1740	punct	", ",trafficking
R871	T1742	T1743	compound	apoptosis,regulation
R872	T1743	T1740	conj	regulation,trafficking
R873	T1744	T1743	punct	", ",regulation
R874	T1745	T1743	cc	and,regulation
R875	T1746	T1747	amod	mitochondrial,integrity
R876	T1747	T1743	conj	integrity,regulation
R877	T1748	T1731	auxpass	was,proposed
R878	T1749	T1750	punct	[,13
R879	T1750	T1731	parataxis	13,proposed
R88	T302	T301	compound	blood,cells
R880	T1751	T1750	punct	],13
R881	T1752	T1731	punct	.,proposed
R882	T1754	T1755	det	The,role
R883	T1755	T1757	nsubjpass	role,understood
R884	T1756	T1755	amod	cellular,role
R885	T1758	T1755	prep	of,role
R886	T1759	T1760	compound	annexin,A7
R887	T1760	T1758	pobj	A7,of
R888	T1761	T1757	auxpass	is,understood
R889	T1762	T1757	neg	not,understood
R89	T303	T298	aux	might,protect
R890	T1763	T1757	advmod	well,understood
R891	T1764	T1757	punct	.,understood
R892	T1766	T1767	nsubjpass	It,thought
R893	T1768	T1767	auxpass	is,thought
R894	T1769	T1770	aux	to,regulate
R895	T1770	T1767	xcomp	regulate,thought
R896	T1771	T1770	cc	and,regulate
R897	T1772	T1770	conj	stabilize,regulate
R898	T1773	T1774	compound	membrane,domains
R899	T1774	T1772	dobj	domains,stabilize
R9	T216	T215	pobj	platelets,in
R90	T304	T298	dobj	themselves,protect
R900	T1775	T1770	cc	and,regulate
R901	T1776	T1777	aux	to,have
R902	T1777	T1770	conj	have,regulate
R903	T1778	T1779	det	a,role
R904	T1779	T1777	dobj	role,have
R905	T1780	T1777	prep	in,have
R906	T1781	T1782	compound	Ca2+,homeostasis
R907	T1782	T1780	pobj	homeostasis,in
R908	T1783	T1782	cc	and,homeostasis
R909	T1784	T1785	npadvmod	Ca2+,dependent
R91	T305	T298	prep	against,protect
R910	T1785	T1787	amod	dependent,pathways
R911	T1786	T1785	punct	-,dependent
R912	T1787	T1782	conj	pathways,homeostasis
R913	T1788	T1787	compound	signaling,pathways
R914	T1789	T1767	punct	.,thought
R915	T1791	T1792	det	These,proposals
R916	T1792	T1793	nsubjpass	proposals,supported
R917	T1794	T1793	auxpass	are,supported
R918	T1795	T1793	agent	by,supported
R919	T1796	T1795	pobj	data,by
R92	T306	T307	det	an,attack
R920	T1797	T1796	acl	obtained,data
R921	T1798	T1797	prep	from,obtained
R922	T1799	T1798	pobj	analysis,from
R923	T1800	T1799	prep	of,analysis
R924	T1801	T1802	compound	annexin,A7
R925	T1802	T1803	npadvmod	A7,deficient
R926	T1803	T1804	amod	deficient,mutants
R927	T1804	T1800	pobj	mutants,of
R928	T1805	T1804	compound	mouse,mutants
R929	T1806	T1793	punct	.,supported
R93	T307	T305	pobj	attack,against
R930	T1808	T1809	nummod	Two,strains
R931	T1809	T1815	nsubjpass	strains,generated
R932	T1810	T1811	nmod	annexin,A7
R933	T1811	T1809	nmod	A7,strains
R934	T1812	T1809	nmod	129Sv,strains
R935	T1919	T1917	cc	and,cells
R936	T1813	T1809	amod	null,strains
R937	T1920	T1917	conj	platelets,cells
R938	T1814	T1809	compound	mice,strains
R939	T1921	T1914	auxpass	were,thought
R94	T308	T307	prep	by,attack
R940	T1816	T1815	auxpass	were,generated
R941	T1817	T1815	advmod	independently,generated
R942	T1922	T1914	neg	not,thought
R943	T1818	T1815	advcl	using,generated
R944	T1819	T1820	det	a,strategy
R945	T1923	T1924	aux	to,contain
R946	T1820	T1818	dobj	strategy,using
R947	T1821	T1820	amod	different,strategy
R948	T1924	T1914	xcomp	contain,thought
R949	T1822	T1815	punct	.,generated
R95	T309	T310	prep	for,components
R950	T1824	T1825	det	The,one
R951	T1925	T1926	compound	annexin,A7
R952	T1825	T1826	nsubj	one,is
R953	T1926	T1924	dobj	A7,contain
R954	T1927	T1914	cc	and,thought
R955	T1928	T1929	advmod	only,recently
R956	T1827	T1825	prep	of,one
R957	T1929	T1930	advmod	recently,reported
R958	T1828	T1827	pobj	Srivastava,of
R959	T1829	T1830	advmod	et,al.
R96	T310	T308	pobj	components,by
R960	T1830	T1828	advmod	al.,Srivastava
R961	T1930	T1914	conj	reported,thought
R962	T1831	T1832	punct	[,16
R963	T1832	T1828	parataxis	16,Srivastava
R964	T1931	T1932	det	the,isoform
R965	T1833	T1832	punct	],16
R966	T1834	T1826	acomp	lethal,is
R967	T1835	T1826	punct	.,is
R968	T1932	T1930	nsubjpass	isoform,reported
R969	T1837	T1838	amod	Heterozygous,mice
R97	T311	T309	pobj	example,for
R970	T1933	T1934	nummod	47,kDa
R971	T1838	T1839	nsubj	mice,exhibit
R972	T1934	T1932	compound	kDa,isoform
R973	T1840	T1841	det	an,defect
R974	T1841	T1839	dobj	defect,exhibit
R975	T1842	T1843	compound	insulin,secretion
R976	T1935	T1930	aux	has,reported
R977	T1843	T1841	compound	secretion,defect
R978	T1844	T1841	cc	and,defect
R979	T1845	T1846	compound	tumor,phenotypes
R98	T312	T310	compound	complement,components
R980	T1936	T1930	auxpass	been,reported
R981	T1846	T1841	conj	phenotypes,defect
R982	T1847	T1839	punct	.,exhibit
R983	T1937	T1930	prep	as,reported
R984	T1849	T1850	det	The,strain
R985	T1850	T1852	nsubj	strain,is
R986	T1851	T1850	amod	null,strain
R987	T1853	T1850	acl	reported,strain
R988	T1938	T1937	pobj	component,as
R989	T1854	T1853	agent	by,reported
R99	T313	T266	punct	.,identified
R990	T1855	T1854	pobj	Herr,by
R991	T1939	T1938	prep	of,component
R992	T1856	T1857	advmod	et,al.
R993	T1857	T1855	advmod	al.,Herr
R994	T1858	T1859	punct	[,17
R995	T1940	T1941	amod	red,cells
R996	T1859	T1853	parataxis	17,reported
R997	T1860	T1859	punct	],17
R998	T1941	T1939	pobj	cells,of
R999	T1861	T1862	amod	viable,healthy

PMC:194730 JSONTXT 9 Projects

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PMC:194730 JSON TXT 9 Projects