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PMC:1941754 / 6545-30700 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1407 9-15 NN denotes Trip13
T1408 16-18 VBZ denotes Is
T1409 19-20 DT denotes a
T1411 21-27 RB denotes Widely
T1412 28-37 VBN denotes Expressed
T1413 38-47 JJ denotes Mammalian
T1410 48-56 NN denotes Ortholog
T1414 57-59 IN denotes of
T1415 60-64 NN denotes PCH2
T1416 65-69 IN denotes with
T1417 70-77 JJ denotes Unusual
T1419 78-90 JJ denotes Phylogenetic
T1418 91-104 NNS denotes Relationships
T1420 104-319 sentence denotes The mammalian ortholog of PCH2, Trip13 (thyroid hormone receptor interacting protein 13), encodes a protein with extensive amino acid homology in regions alignable to the yeast and worm orthologs (Figure S1) [12]).
T1421 105-108 DT denotes The
T1423 109-118 JJ denotes mammalian
T1422 119-127 NN denotes ortholog
T1425 128-130 IN denotes of
T1426 131-135 NN denotes PCH2
T1427 135-137 , denotes ,
T1428 137-143 NN denotes Trip13
T1429 144-145 -LRB- denotes (
T1430 145-152 NN denotes thyroid
T1432 153-160 NN denotes hormone
T1431 161-169 NN denotes receptor
T1433 170-181 VBG denotes interacting
T1434 182-189 NN denotes protein
T1435 190-192 CD denotes 13
T1436 192-193 -RRB- denotes )
T1437 193-195 , denotes ,
T1424 195-202 VBZ denotes encodes
T1438 203-204 DT denotes a
T1439 205-212 NN denotes protein
T1440 213-217 IN denotes with
T1441 218-227 JJ denotes extensive
T1443 228-233 NN denotes amino
T1444 234-238 NN denotes acid
T1442 239-247 NN denotes homology
T1445 248-250 IN denotes in
T1446 251-258 NNS denotes regions
T1447 259-268 JJ denotes alignable
T1448 269-271 IN denotes to
T1449 272-275 DT denotes the
T1451 276-281 NN denotes yeast
T1452 282-285 CC denotes and
T1453 286-290 NN denotes worm
T1450 291-300 NNS denotes orthologs
T1454 301-302 -LRB- denotes (
T1456 302-308 NN denotes Figure
T1455 309-311 NN denotes S1
T1457 311-312 -RRB- denotes )
T1458 313-314 -LRB- denotes [
T1459 314-316 CD denotes 12
T1460 316-317 -RRB- denotes ]
T1461 317-318 -RRB- denotes )
T1462 318-319 . denotes .
T1463 319-538 sentence denotes Interestingly, phylogenetic analysis of TRIP13/Pch2p shows that the mammalian protein clusters more closely to plants than it does to the evolutionarily more closely related worms and flies (Figure 1A; see Discussion).
T1464 320-333 RB denotes Interestingly
T1466 333-335 , denotes ,
T1467 335-347 JJ denotes phylogenetic
T1468 348-356 NN denotes analysis
T1469 357-359 IN denotes of
T1470 360-366 NN denotes TRIP13
T1472 366-367 HYPH denotes /
T1471 367-372 NN denotes Pch2p
T1465 373-378 VBZ denotes shows
T1473 379-383 IN denotes that
T1475 384-387 DT denotes the
T1477 388-397 JJ denotes mammalian
T1476 398-405 NN denotes protein
T1474 406-414 VBZ denotes clusters
T1478 415-419 RBR denotes more
T1479 420-427 RB denotes closely
T1480 428-430 IN denotes to
T1481 431-437 NNS denotes plants
T1482 438-442 IN denotes than
T1484 443-445 PRP denotes it
T1485 446-450 VBZ denotes does
T1483 451-453 IN denotes to
T1486 454-457 DT denotes the
T1488 458-472 RB denotes evolutionarily
T1490 473-477 RBR denotes more
T1491 478-485 RB denotes closely
T1489 486-493 VBN denotes related
T1487 494-499 NNS denotes worms
T1492 500-503 CC denotes and
T1493 504-509 NNS denotes flies
T1494 510-511 -LRB- denotes (
T1496 511-517 NN denotes Figure
T1495 518-520 NN denotes 1A
T1497 520-521 : denotes ;
T1498 522-525 VB denotes see
T1499 526-536 NN denotes Discussion
T1500 536-537 -RRB- denotes )
T1501 537-538 . denotes .
T1502 538-812 sentence denotes Semi-quantitative reverse-transcriptase PCR (RT-PCR) analysis showed Trip13 mRNA to be expressed in a variety of embryonic and adult tissues, including testis (Figure 1B), consistent with mouse and human EST data summarized in Unigene (http://www.ncbi.nlm.nih.gov/UniGene).
T1503 539-556 JJ denotes Semi-quantitative
T1505 557-564 NN denotes reverse
T1507 564-565 HYPH denotes -
T1506 565-578 NN denotes transcriptase
T1508 579-582 NN denotes PCR
T1509 583-584 -LRB- denotes (
T1510 584-586 NN denotes RT
T1512 586-587 HYPH denotes -
T1511 587-590 NN denotes PCR
T1513 590-591 -RRB- denotes )
T1504 592-600 NN denotes analysis
T1514 601-607 VBD denotes showed
T1515 608-614 NN denotes Trip13
T1516 615-619 NN denotes mRNA
T1518 620-622 TO denotes to
T1519 623-625 VB denotes be
T1517 626-635 VBN denotes expressed
T1520 636-638 IN denotes in
T1521 639-640 DT denotes a
T1522 641-648 NN denotes variety
T1523 649-651 IN denotes of
T1524 652-661 JJ denotes embryonic
T1526 662-665 CC denotes and
T1527 666-671 JJ denotes adult
T1525 672-679 NNS denotes tissues
T1528 679-681 , denotes ,
T1529 681-690 VBG denotes including
T1530 691-697 NN denotes testis
T1531 698-699 -LRB- denotes (
T1533 699-705 NN denotes Figure
T1532 706-708 NN denotes 1B
T1534 708-709 -RRB- denotes )
T1535 709-711 , denotes ,
T1536 711-721 JJ denotes consistent
T1537 722-726 IN denotes with
T1538 727-732 NN denotes mouse
T1540 733-736 CC denotes and
T1541 737-742 JJ denotes human
T1539 743-746 NN denotes EST
T1542 747-751 NNS denotes data
T1543 752-762 VBN denotes summarized
T1544 763-765 IN denotes in
T1545 766-773 NNP denotes Unigene
T1546 774-775 -LRB- denotes (
T1547 775-810 NN denotes http://www.ncbi.nlm.nih.gov/UniGene
T1548 810-811 -RRB- denotes )
T1549 811-812 . denotes .
T1550 812-873 sentence denotes It is also highly expressed in human and mouse oocytes [17].
T1551 813-815 PRP denotes It
T1553 816-818 VBZ denotes is
T1554 819-823 RB denotes also
T1555 824-830 RB denotes highly
T1552 831-840 VBN denotes expressed
T1556 841-843 IN denotes in
T1557 844-849 JJ denotes human
T1559 850-853 CC denotes and
T1558 854-859 NN denotes mouse
T1560 860-867 NNS denotes oocytes
T1561 868-869 -LRB- denotes [
T1562 869-871 CD denotes 17
T1563 871-872 -RRB- denotes ]
T1564 872-873 . denotes .
T10169 884-887 DT denotes The
T10171 888-893 NN denotes Mouse
T10172 894-898 NN denotes PCH2
T10170 899-907 NN denotes Ortholog
T10173 908-914 NN denotes TRIP13
T10174 915-918 CC denotes and
T10175 919-929 NN denotes Expression
T10176 930-932 IN denotes in
T10177 933-937 JJ denotes Wild
T10178 938-942 NN denotes Type
T10179 943-946 CC denotes and
T10180 947-953 NN denotes Mutant
T10181 953-1010 sentence denotes (A) Phylogenetic tree of presumed PCH2/TRIP13 orthologs.
T10182 954-955 -LRB- denotes (
T10183 955-956 LS denotes A
T10185 956-957 -RRB- denotes )
T10186 958-970 JJ denotes Phylogenetic
T10184 971-975 NN denotes tree
T10187 976-978 IN denotes of
T10188 979-987 JJ denotes presumed
T10190 988-992 NN denotes PCH2
T10192 992-993 HYPH denotes /
T10191 993-999 NN denotes TRIP13
T10189 1000-1009 NNS denotes orthologs
T10193 1009-1010 . denotes .
T10194 1010-1091 sentence denotes The database sequence accession number of each protein is presented in Table S1.
T10195 1011-1014 DT denotes The
T10197 1015-1023 NN denotes database
T10198 1024-1032 NN denotes sequence
T10199 1033-1042 NN denotes accession
T10196 1043-1049 NN denotes number
T10201 1050-1052 IN denotes of
T10202 1053-1057 DT denotes each
T10203 1058-1065 NN denotes protein
T10204 1066-1068 VBZ denotes is
T10200 1069-1078 VBN denotes presented
T10205 1079-1081 IN denotes in
T10206 1082-1087 NN denotes Table
T10207 1088-1090 NN denotes S1
T10208 1090-1091 . denotes .
T10209 1091-1155 sentence denotes Numbers shown are bootstrap values (see Materials and Methods).
T10210 1092-1099 NNS denotes Numbers
T10212 1100-1105 VBN denotes shown
T10211 1106-1109 VBP denotes are
T10213 1110-1119 NN denotes bootstrap
T10214 1120-1126 NNS denotes values
T10215 1127-1128 -LRB- denotes (
T10216 1128-1131 VB denotes see
T10217 1132-1141 NNS denotes Materials
T10218 1142-1145 CC denotes and
T10219 1146-1153 NNS denotes Methods
T10220 1153-1154 -RRB- denotes )
T10221 1154-1155 . denotes .
T10222 1155-1340 sentence denotes The clustering was the same regardless of whether the whole entire AA sequence or trimmed sequences (where regions showing little conservation were removed) were used for the analysis.
T10223 1156-1159 DT denotes The
T10224 1160-1170 NN denotes clustering
T10225 1171-1174 VBD denotes was
T10226 1175-1178 DT denotes the
T10227 1179-1183 JJ denotes same
T10228 1184-1194 RB denotes regardless
T10229 1195-1197 IN denotes of
T10230 1198-1205 IN denotes whether
T10232 1206-1209 DT denotes the
T10234 1210-1215 JJ denotes whole
T10235 1216-1222 JJ denotes entire
T10236 1223-1225 NN denotes AA
T10233 1226-1234 NN denotes sequence
T10237 1235-1237 CC denotes or
T10238 1238-1245 VBN denotes trimmed
T10239 1246-1255 NNS denotes sequences
T10240 1256-1257 -LRB- denotes (
T10241 1257-1262 WRB denotes where
T10243 1263-1270 NNS denotes regions
T10244 1271-1278 VBG denotes showing
T10245 1279-1285 JJ denotes little
T10246 1286-1298 NN denotes conservation
T10247 1299-1303 VBD denotes were
T10242 1304-1311 VBN denotes removed
T10248 1311-1312 -RRB- denotes )
T10249 1313-1317 VBD denotes were
T10231 1318-1322 VBN denotes used
T10250 1323-1326 IN denotes for
T10251 1327-1330 DT denotes the
T10252 1331-1339 NN denotes analysis
T10253 1339-1340 . denotes .
T10254 1340-1477 sentence denotes Major eukaryotic groups are indicated in color, with deuterostomia in blue, plants in green, protostomia in purple, and fungi in maroon.
T10255 1341-1346 JJ denotes Major
T10257 1347-1357 JJ denotes eukaryotic
T10256 1358-1364 NNS denotes groups
T10259 1365-1368 VBP denotes are
T10258 1369-1378 VBN denotes indicated
T10260 1379-1381 IN denotes in
T10261 1382-1387 NN denotes color
T10262 1387-1389 , denotes ,
T10263 1389-1393 IN denotes with
T10265 1394-1407 NN denotes deuterostomia
T10264 1408-1410 IN denotes in
T10266 1411-1415 NN denotes blue
T10267 1415-1417 , denotes ,
T10268 1417-1423 NNS denotes plants
T10269 1424-1426 IN denotes in
T10270 1427-1432 NN denotes green
T10271 1432-1434 , denotes ,
T10272 1434-1445 NN denotes protostomia
T10273 1446-1448 IN denotes in
T10274 1449-1455 NN denotes purple
T10275 1455-1457 , denotes ,
T10276 1457-1460 CC denotes and
T10277 1461-1466 NNS denotes fungi
T10278 1467-1469 IN denotes in
T10279 1470-1476 NN denotes maroon
T10280 1476-1477 . denotes .
T10281 1477-1698 sentence denotes (B) Amplification products of cDNA from the following tissues: 1, heart; 2, brain; 3, spleen; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; 8, testis; 9, E7 embryo; 10, E11 embryo; 11, E15 embryo; and 12, E17 embryo.
T10282 1478-1479 -LRB- denotes (
T10283 1479-1480 LS denotes B
T10285 1480-1481 -RRB- denotes )
T10286 1482-1495 NN denotes Amplification
T10284 1496-1504 NNS denotes products
T10287 1505-1507 IN denotes of
T10288 1508-1512 NN denotes cDNA
T10289 1513-1517 IN denotes from
T10290 1518-1521 DT denotes the
T10292 1522-1531 JJ denotes following
T10291 1532-1539 NNS denotes tissues
T10293 1539-1541 : denotes :
T10294 1541-1542 LS denotes 1
T10296 1542-1544 , denotes ,
T10295 1544-1549 NN denotes heart
T10297 1549-1550 : denotes ;
T10298 1551-1552 LS denotes 2
T10300 1552-1554 , denotes ,
T10299 1554-1559 NN denotes brain
T10301 1559-1560 : denotes ;
T10302 1561-1562 LS denotes 3
T10304 1562-1564 , denotes ,
T10303 1564-1570 NN denotes spleen
T10305 1570-1571 : denotes ;
T10306 1572-1573 LS denotes 4
T10308 1573-1575 , denotes ,
T10307 1575-1579 NN denotes lung
T10309 1579-1580 : denotes ;
T10310 1581-1582 LS denotes 5
T10312 1582-1584 , denotes ,
T10311 1584-1589 NN denotes liver
T10313 1589-1590 : denotes ;
T10314 1591-1592 LS denotes 6
T10316 1592-1594 , denotes ,
T10317 1594-1602 JJ denotes skeletal
T10315 1603-1609 NN denotes muscle
T10318 1609-1610 : denotes ;
T10319 1611-1612 LS denotes 7
T10321 1612-1614 , denotes ,
T10320 1614-1620 NN denotes kidney
T10322 1620-1621 : denotes ;
T10323 1622-1623 LS denotes 8
T10325 1623-1625 , denotes ,
T10324 1625-1631 NN denotes testis
T10326 1631-1632 : denotes ;
T10327 1633-1634 LS denotes 9
T10329 1634-1636 , denotes ,
T10330 1636-1638 NN denotes E7
T10328 1639-1645 NN denotes embryo
T10331 1645-1646 : denotes ;
T10332 1647-1649 LS denotes 10
T10334 1649-1651 , denotes ,
T10335 1651-1654 NN denotes E11
T10333 1655-1661 NN denotes embryo
T10336 1661-1662 : denotes ;
T10337 1663-1665 LS denotes 11
T10339 1665-1667 , denotes ,
T10340 1667-1670 NN denotes E15
T10338 1671-1677 NN denotes embryo
T10341 1677-1678 : denotes ;
T10342 1679-1682 CC denotes and
T10343 1683-1685 LS denotes 12
T10345 1685-1687 , denotes ,
T10346 1687-1690 NN denotes E17
T10344 1691-1697 NN denotes embryo
T10347 1697-1698 . denotes .
T10348 1698-1774 sentence denotes (C) Intron–exon structure of TRIP13 and insertion site of gene-trap vector.
T10349 1699-1700 -LRB- denotes (
T10350 1700-1701 LS denotes C
T10352 1701-1702 -RRB- denotes )
T10353 1703-1709 NN denotes Intron
T10355 1709-1710 HYPH denotes –
T10354 1710-1714 NN denotes exon
T10351 1715-1724 NN denotes structure
T10356 1725-1727 IN denotes of
T10357 1728-1734 NN denotes TRIP13
T10358 1735-1738 CC denotes and
T10359 1739-1748 NN denotes insertion
T10360 1749-1753 NN denotes site
T10361 1754-1756 IN denotes of
T10362 1757-1761 NN denotes gene
T10364 1761-1762 HYPH denotes -
T10363 1762-1766 NN denotes trap
T10365 1767-1773 NN denotes vector
T10366 1773-1774 . denotes .
T10367 1774-1862 sentence denotes See Materials and Methods for details on how the precise insertion site was identified.
T10368 1775-1778 VB denotes See
T10369 1779-1788 NNS denotes Materials
T10370 1789-1792 CC denotes and
T10371 1793-1800 NNS denotes Methods
T10372 1801-1804 IN denotes for
T10373 1805-1812 NNS denotes details
T10374 1813-1815 IN denotes on
T10375 1816-1819 WRB denotes how
T10377 1820-1823 DT denotes the
T10379 1824-1831 JJ denotes precise
T10380 1832-1841 NN denotes insertion
T10378 1842-1846 NN denotes site
T10381 1847-1850 VBD denotes was
T10376 1851-1861 VBN denotes identified
T10382 1861-1862 . denotes .
T10383 1862-1925 sentence denotes (D) RT-PCR of Trip13 and a control gene Med31 from testis RNA.
T10384 1863-1864 -LRB- denotes (
T10385 1864-1865 LS denotes D
T10387 1865-1866 -RRB- denotes )
T10388 1867-1869 NN denotes RT
T10389 1869-1870 HYPH denotes -
T10386 1870-1873 NN denotes PCR
T10390 1874-1876 IN denotes of
T10391 1877-1883 NN denotes Trip13
T10392 1884-1887 CC denotes and
T10393 1888-1889 DT denotes a
T10395 1890-1897 NN denotes control
T10396 1898-1902 NN denotes gene
T10394 1903-1908 NN denotes Med31
T10397 1909-1913 IN denotes from
T10398 1914-1920 NN denotes testis
T10399 1921-1924 NN denotes RNA
T10400 1924-1925 . denotes .
T10401 1925-2014 sentence denotes The Trip13 primers are situated in the first and last exons (see Materials and Methods).
T10402 1926-1929 DT denotes The
T10404 1930-1936 NN denotes Trip13
T10403 1937-1944 NNS denotes primers
T10406 1945-1948 VBP denotes are
T10405 1949-1957 VBN denotes situated
T10407 1958-1960 IN denotes in
T10408 1961-1964 DT denotes the
T10410 1965-1970 JJ denotes first
T10411 1971-1974 CC denotes and
T10412 1975-1979 JJ denotes last
T10409 1980-1985 NNS denotes exons
T10413 1986-1987 -LRB- denotes (
T10414 1987-1990 VB denotes see
T10415 1991-2000 NNS denotes Materials
T10416 2001-2004 CC denotes and
T10417 2005-2012 NNS denotes Methods
T10418 2012-2013 -RRB- denotes )
T10419 2013-2014 . denotes .
T10420 2014-2085 sentence denotes (E) Western blot analysis of testis protein with anti-TRIP13 antibody.
T10421 2015-2016 -LRB- denotes (
T10422 2016-2017 LS denotes E
T10424 2017-2018 -RRB- denotes )
T10425 2019-2026 NNP denotes Western
T10426 2027-2031 NN denotes blot
T10423 2032-2040 NN denotes analysis
T10427 2041-2043 IN denotes of
T10428 2044-2050 NN denotes testis
T10429 2051-2058 NN denotes protein
T10430 2059-2063 IN denotes with
T10431 2064-2075 JJ denotes anti-TRIP13
T10432 2076-2084 NN denotes antibody
T10433 2084-2085 . denotes .
T10434 2085-2163 sentence denotes The blot was later probed with anti-alpha tubulin actin as a loading control.
T10435 2086-2089 DT denotes The
T10436 2090-2094 NN denotes blot
T10438 2095-2098 VBD denotes was
T10439 2099-2104 RB denotes later
T10437 2105-2111 VBN denotes probed
T10440 2112-2116 IN denotes with
T10441 2117-2127 JJ denotes anti-alpha
T10443 2128-2135 NN denotes tubulin
T10442 2136-2141 NN denotes actin
T10444 2142-2144 IN denotes as
T10445 2145-2146 DT denotes a
T10447 2147-2154 NN denotes loading
T10446 2155-2162 NN denotes control
T10448 2162-2163 . denotes .
T10449 2163-2203 sentence denotes The expected TRIP13 protein is ∼48 KDa.
T10450 2164-2167 DT denotes The
T10452 2168-2176 JJ denotes expected
T10453 2177-2183 NN denotes TRIP13
T10451 2184-2191 NN denotes protein
T10454 2192-2194 VBZ denotes is
T10455 2195-2196 SYM denotes ∼
T10456 2196-2198 CD denotes 48
T10457 2199-2202 NN denotes KDa
T10458 2202-2203 . denotes .
T10459 2203-2241 sentence denotes (F) Localization of TRIP13 in testes.
T10460 2204-2205 -LRB- denotes (
T10461 2205-2206 LS denotes F
T10463 2206-2207 -RRB- denotes )
T10462 2208-2220 NN denotes Localization
T10464 2221-2223 IN denotes of
T10465 2224-2230 NN denotes TRIP13
T10466 2231-2233 IN denotes in
T10467 2234-2240 NNS denotes testes
T10468 2240-2241 . denotes .
T10469 2241-2403 sentence denotes Wild-type (top) and mutant (bottom) testis sections were probed with chicken anti-TRIP13, and detected with HRP-conjugated anti-chicken IgG (brown/red staining).
T10470 2242-2246 JJ denotes Wild
T10472 2246-2247 HYPH denotes -
T10471 2247-2251 NN denotes type
T10474 2252-2253 -LRB- denotes (
T10475 2253-2256 JJ denotes top
T10476 2256-2257 -RRB- denotes )
T10477 2258-2261 CC denotes and
T10478 2262-2268 NN denotes mutant
T10479 2269-2270 -LRB- denotes (
T10480 2270-2276 JJ denotes bottom
T10481 2276-2277 -RRB- denotes )
T10473 2278-2284 NN denotes testis
T10482 2285-2293 NNS denotes sections
T10484 2294-2298 VBD denotes were
T10483 2299-2305 VBN denotes probed
T10485 2306-2310 IN denotes with
T10486 2311-2318 NN denotes chicken
T10487 2319-2330 JJ denotes anti-TRIP13
T10488 2330-2332 , denotes ,
T10489 2332-2335 CC denotes and
T10490 2336-2344 VBN denotes detected
T10491 2345-2349 IN denotes with
T10492 2350-2353 NN denotes HRP
T10494 2353-2354 HYPH denotes -
T10493 2354-2364 VBN denotes conjugated
T10496 2365-2377 JJ denotes anti-chicken
T10495 2378-2381 NN denotes IgG
T10497 2382-2383 -LRB- denotes (
T10499 2383-2388 JJ denotes brown
T10501 2388-2389 HYPH denotes /
T10500 2389-2392 JJ denotes red
T10498 2393-2401 NN denotes staining
T10502 2401-2402 -RRB- denotes )
T10503 2402-2403 . denotes .
T10504 2403-2596 sentence denotes Expression in WT was most prominent in the nuclei of Type B spermatogonia (Sg), leptotene spermatocytes (LS), and early pachytene spermatocytes (PS), but not late pachytene spermatocytes (LP).
T10505 2404-2414 NN denotes Expression
T10507 2415-2417 IN denotes in
T10508 2418-2420 NN denotes WT
T10506 2421-2424 VBD denotes was
T10509 2425-2429 RBS denotes most
T10510 2430-2439 JJ denotes prominent
T10511 2440-2442 IN denotes in
T10512 2443-2446 DT denotes the
T10513 2447-2453 NNS denotes nuclei
T10514 2454-2456 IN denotes of
T10515 2457-2461 NN denotes Type
T10516 2462-2463 NN denotes B
T10517 2464-2477 NNS denotes spermatogonia
T10518 2478-2479 -LRB- denotes (
T10519 2479-2481 NN denotes Sg
T10520 2481-2482 -RRB- denotes )
T10521 2482-2484 , denotes ,
T10522 2484-2493 NN denotes leptotene
T10523 2494-2507 NNS denotes spermatocytes
T10524 2508-2509 -LRB- denotes (
T10525 2509-2511 NNS denotes LS
T10526 2511-2512 -RRB- denotes )
T10527 2512-2514 , denotes ,
T10528 2514-2517 CC denotes and
T10529 2518-2523 JJ denotes early
T10531 2524-2533 NN denotes pachytene
T10530 2534-2547 NNS denotes spermatocytes
T10532 2548-2549 -LRB- denotes (
T10533 2549-2551 NN denotes PS
T10534 2551-2552 -RRB- denotes )
T10535 2552-2554 , denotes ,
T10536 2554-2557 CC denotes but
T10537 2558-2561 RB denotes not
T10538 2562-2566 JJ denotes late
T10540 2567-2576 NN denotes pachytene
T10539 2577-2590 NNS denotes spermatocytes
T10541 2591-2592 -LRB- denotes (
T10542 2592-2594 NN denotes LP
T10543 2594-2595 -RRB- denotes )
T10544 2595-2596 . denotes .
T10545 2596-2704 sentence denotes No nuclear staining was seen in mutant testis sections, although reddish cytoplasmic background is present.
T10546 2597-2599 DT denotes No
T10548 2600-2607 JJ denotes nuclear
T10547 2608-2616 NN denotes staining
T10550 2617-2620 VBD denotes was
T10549 2621-2625 VBN denotes seen
T10551 2626-2628 IN denotes in
T10552 2629-2635 NN denotes mutant
T10554 2636-2642 NN denotes testis
T10553 2643-2651 NNS denotes sections
T10555 2651-2653 , denotes ,
T10556 2653-2661 IN denotes although
T10558 2662-2669 JJ denotes reddish
T10560 2670-2681 JJ denotes cytoplasmic
T10559 2682-2692 NN denotes background
T10557 2693-2695 VBZ denotes is
T10561 2696-2703 JJ denotes present
T10562 2703-2704 . denotes .
T10563 2704-2821 sentence denotes Identification of cell types was judged in part by estimating the epithelial stage of the tubules as described [67].
T10564 2705-2719 NN denotes Identification
T10566 2720-2722 IN denotes of
T10567 2723-2727 NN denotes cell
T10568 2728-2733 NNS denotes types
T10569 2734-2737 VBD denotes was
T10565 2738-2744 VBN denotes judged
T10570 2745-2747 IN denotes in
T10571 2748-2752 JJ denotes part
T10572 2753-2755 IN denotes by
T10573 2756-2766 VBG denotes estimating
T10574 2767-2770 DT denotes the
T10576 2771-2781 JJ denotes epithelial
T10575 2782-2787 NN denotes stage
T10577 2788-2790 IN denotes of
T10578 2791-2794 DT denotes the
T10579 2795-2802 NNS denotes tubules
T10580 2803-2805 IN denotes as
T10581 2806-2815 VBN denotes described
T10582 2816-2817 -LRB- denotes [
T10583 2817-2819 CD denotes 67
T10584 2819-2820 -RRB- denotes ]
T10585 2820-2821 . denotes .
T10586 2821-2878 sentence denotes (G) TRIP13 localization in surface-spread spermatocytes.
T10587 2822-2823 -LRB- denotes (
T10588 2823-2824 LS denotes G
T10590 2824-2825 -RRB- denotes )
T10591 2826-2832 NN denotes TRIP13
T10589 2833-2845 NN denotes localization
T10592 2846-2848 IN denotes in
T10593 2849-2856 NN denotes surface
T10595 2856-2857 HYPH denotes -
T10594 2857-2863 NN denotes spread
T10596 2864-2877 NNS denotes spermatocytes
T10597 2877-2878 . denotes .
T10598 2878-2946 sentence denotes Preparations were immunolabeled with anti-SYCP3 (S) and TRIP13 (T).
T10599 2879-2891 NNS denotes Preparations
T10601 2892-2896 VBD denotes were
T10600 2897-2910 VBN denotes immunolabeled
T10602 2911-2915 IN denotes with
T10603 2916-2926 JJ denotes anti-SYCP3
T10604 2927-2928 -LRB- denotes (
T10605 2928-2929 NN denotes S
T10606 2929-2930 -RRB- denotes )
T10607 2931-2934 CC denotes and
T10608 2935-2941 NN denotes TRIP13
T10609 2942-2943 -LRB- denotes (
T10610 2943-2944 NN denotes T
T10611 2944-2945 -RRB- denotes )
T10612 2945-2946 . denotes .
T10613 2946-3062 sentence denotes Both individual and merged images are shown for leptotene (Lep), zygotene (Zyg), and pachytene (Pac) spermatocytes.
T10614 2947-2951 CC denotes Both
T10615 2952-2962 JJ denotes individual
T10617 2963-2966 CC denotes and
T10618 2967-2973 VBN denotes merged
T10616 2974-2980 NNS denotes images
T10620 2981-2984 VBP denotes are
T10619 2985-2990 VBN denotes shown
T10621 2991-2994 IN denotes for
T10622 2995-3004 NN denotes leptotene
T10624 3005-3006 -LRB- denotes (
T10625 3006-3009 NN denotes Lep
T10626 3009-3010 -RRB- denotes )
T10627 3010-3012 , denotes ,
T10628 3012-3020 NN denotes zygotene
T10629 3021-3022 -LRB- denotes (
T10630 3022-3025 NN denotes Zyg
T10631 3025-3026 -RRB- denotes )
T10632 3026-3028 , denotes ,
T10633 3028-3031 CC denotes and
T10634 3032-3041 NN denotes pachytene
T10635 3042-3043 -LRB- denotes (
T10636 3043-3046 NN denotes Pac
T10637 3046-3047 -RRB- denotes )
T10623 3048-3061 NNS denotes spermatocytes
T10638 3061-3062 . denotes .
T10639 3062-3105 sentence denotes Nuclear staining was absent in the mutant.
T10640 3063-3070 JJ denotes Nuclear
T10641 3071-3079 NN denotes staining
T10642 3080-3083 VBD denotes was
T10643 3084-3090 JJ denotes absent
T10644 3091-3093 IN denotes in
T10645 3094-3097 DT denotes the
T10646 3098-3104 NN denotes mutant
T10647 3104-3105 . denotes .
T1725 3107-3117 NN denotes Generation
T1726 3118-3120 IN denotes of
T1727 3121-3127 NN denotes Trip13
T1729 3128-3134 NN denotes Mutant
T1728 3135-3139 NNS denotes Mice
T1730 3139-3289 sentence denotes To explore the function of TRIP13 in mammals, we generated mice with a gene trap-disrupted allele, Trip13RRB047 (Figure 1C; abbreviated as Trip13Gt).
T1731 3140-3142 TO denotes To
T1732 3143-3150 VB denotes explore
T1734 3151-3154 DT denotes the
T1735 3155-3163 NN denotes function
T1736 3164-3166 IN denotes of
T1737 3167-3173 NN denotes TRIP13
T1738 3174-3176 IN denotes in
T1739 3177-3184 NNS denotes mammals
T1740 3184-3186 , denotes ,
T1741 3186-3188 PRP denotes we
T1733 3189-3198 VBD denotes generated
T1742 3199-3203 NNS denotes mice
T1743 3204-3208 IN denotes with
T1744 3209-3210 DT denotes a
T1746 3211-3215 NN denotes gene
T1747 3216-3220 NN denotes trap
T1749 3220-3221 HYPH denotes -
T1748 3221-3230 VBN denotes disrupted
T1745 3231-3237 NN denotes allele
T1750 3237-3239 , denotes ,
T1751 3239-3251 NN denotes Trip13RRB047
T1752 3252-3253 -LRB- denotes (
T1754 3253-3259 NN denotes Figure
T1753 3260-3262 NN denotes 1C
T1755 3262-3263 : denotes ;
T1756 3264-3275 VBN denotes abbreviated
T1757 3276-3278 IN denotes as
T1758 3279-3287 NN denotes Trip13Gt
T1759 3287-3288 -RRB- denotes )
T1760 3288-3289 . denotes .
T1761 3289-3481 sentence denotes Heterozygotes were normal in all respects, but homozygotes were present at ∼2/3 the expected ratio from intercrosses between heterozygotes (91 Trip13 +/+, 183 Trip13Gt/+, and 61 Trip13Gt/Gt).
T1762 3290-3303 NNS denotes Heterozygotes
T1763 3304-3308 VBD denotes were
T1764 3309-3315 JJ denotes normal
T1765 3316-3318 IN denotes in
T1766 3319-3322 DT denotes all
T1767 3323-3331 NNS denotes respects
T1768 3331-3333 , denotes ,
T1769 3333-3336 CC denotes but
T1770 3337-3348 NNS denotes homozygotes
T1771 3349-3353 VBD denotes were
T1772 3354-3361 JJ denotes present
T1773 3362-3364 IN denotes at
T1774 3365-3366 SYM denotes ∼
T1776 3366-3367 CD denotes 2
T1777 3367-3368 SYM denotes /
T1775 3368-3369 CD denotes 3
T1779 3370-3373 DT denotes the
T1780 3374-3382 VBN denotes expected
T1778 3383-3388 NN denotes ratio
T1781 3389-3393 IN denotes from
T1782 3394-3406 NNS denotes intercrosses
T1783 3407-3414 IN denotes between
T1784 3415-3428 NNS denotes heterozygotes
T1785 3429-3430 -LRB- denotes (
T1786 3430-3432 CD denotes 91
T1787 3433-3439 NN denotes Trip13
T1788 3440-3441 SYM denotes +
T1789 3441-3442 HYPH denotes /
T1790 3442-3443 SYM denotes +
T1791 3443-3445 , denotes ,
T1792 3445-3448 CD denotes 183
T1793 3449-3457 NN denotes Trip13Gt
T1794 3457-3458 HYPH denotes /
T1795 3458-3459 SYM denotes +
T1796 3459-3461 , denotes ,
T1797 3461-3464 CC denotes and
T1798 3465-3467 CD denotes 61
T1800 3468-3476 NN denotes Trip13Gt
T1801 3476-3477 HYPH denotes /
T1799 3477-3479 NN denotes Gt
T1802 3479-3480 -RRB- denotes )
T1803 3480-3481 . denotes .
T1804 3481-3614 sentence denotes Since >90% of prewean mice that died were mutant homozygotes, this discrepancy is apparently due to a partially penetrant lethality.
T1805 3482-3487 IN denotes Since
T1807 3488-3489 SYM denotes >
T1808 3489-3491 CD denotes 90
T1809 3491-3492 NN denotes %
T1810 3493-3495 IN denotes of
T1811 3496-3503 JJ denotes prewean
T1812 3504-3508 NNS denotes mice
T1813 3509-3513 WDT denotes that
T1814 3514-3518 VBD denotes died
T1806 3519-3523 VBD denotes were
T1816 3524-3530 NN denotes mutant
T1817 3531-3542 NNS denotes homozygotes
T1818 3542-3544 , denotes ,
T1819 3544-3548 DT denotes this
T1820 3549-3560 NN denotes discrepancy
T1815 3561-3563 VBZ denotes is
T1821 3564-3574 RB denotes apparently
T1822 3575-3578 IN denotes due
T1823 3579-3581 IN denotes to
T1824 3582-3583 DT denotes a
T1826 3584-3593 RB denotes partially
T1827 3594-3603 JJ denotes penetrant
T1825 3604-3613 NN denotes lethality
T1828 3613-3614 . denotes .
T1829 3614-3670 sentence denotes Most surviving Trip13Gt/Gt animals were grossly normal.
T1830 3615-3619 JJS denotes Most
T1832 3620-3629 VBG denotes surviving
T1833 3630-3638 NN denotes Trip13Gt
T1835 3638-3639 HYPH denotes /
T1834 3639-3641 NN denotes Gt
T1831 3642-3649 NNS denotes animals
T1836 3650-3654 VBD denotes were
T1837 3655-3662 RB denotes grossly
T1838 3663-3669 JJ denotes normal
T1839 3669-3670 . denotes .
T1840 3670-3826 sentence denotes However, homozygotes that were semi-congenic (N4) on the C57BL/6J strain were often markedly smaller and/or had kinked or shorter tails (Figure 2A and 2B).
T1841 3671-3678 RB denotes However
T1843 3678-3680 , denotes ,
T1844 3680-3691 NNS denotes homozygotes
T1845 3692-3696 WDT denotes that
T1846 3697-3701 VBD denotes were
T1847 3702-3715 JJ denotes semi-congenic
T1848 3716-3717 -LRB- denotes (
T1849 3717-3719 NN denotes N4
T1850 3719-3720 -RRB- denotes )
T1851 3721-3723 IN denotes on
T1852 3724-3727 DT denotes the
T1854 3728-3733 NN denotes C57BL
T1856 3733-3734 HYPH denotes /
T1855 3734-3736 NN denotes 6J
T1853 3737-3743 NN denotes strain
T1842 3744-3748 VBD denotes were
T1857 3749-3754 RB denotes often
T1858 3755-3763 RB denotes markedly
T1859 3764-3771 JJR denotes smaller
T1860 3772-3775 CC denotes and
T1861 3775-3776 HYPH denotes /
T1862 3776-3778 CC denotes or
T1863 3779-3782 VBD denotes had
T1864 3783-3789 VBN denotes kinked
T1866 3790-3792 CC denotes or
T1867 3793-3800 JJR denotes shorter
T1865 3801-3806 NNS denotes tails
T1868 3807-3808 -LRB- denotes (
T1870 3808-3814 NN denotes Figure
T1869 3815-3817 NN denotes 2A
T1871 3818-3821 CC denotes and
T1872 3822-3824 NN denotes 2B
T1873 3824-3825 -RRB- denotes )
T1874 3825-3826 . denotes .
T1875 3826-3827 sentence denotes
T10698 3837-3850 JJ denotes Developmental
T10699 3851-3861 NNS denotes Phenotypes
T10700 3862-3864 IN denotes of
T10701 3865-3871 NN denotes Trip13
T10702 3872-3878 NN denotes Mutant
T10703 3879-3883 NNS denotes Mice
T10704 3883-3919 sentence denotes (A) Shown are 21-d-old littermates.
T10705 3884-3885 -LRB- denotes (
T10706 3885-3886 LS denotes A
T10708 3886-3887 -RRB- denotes )
T10709 3888-3893 VBN denotes Shown
T10707 3894-3897 VBP denotes are
T10710 3898-3900 CD denotes 21
T10712 3900-3901 HYPH denotes -
T10711 3901-3902 NN denotes d
T10714 3902-3903 HYPH denotes -
T10713 3903-3906 JJ denotes old
T10715 3907-3918 NNS denotes littermates
T10716 3918-3919 . denotes .
T10717 3919-3989 sentence denotes Note the shortened tail in the mutant, but overall similar body size.
T10718 3920-3924 VB denotes Note
T10719 3925-3928 DT denotes the
T10721 3929-3938 VBN denotes shortened
T10720 3939-3943 NN denotes tail
T10722 3944-3946 IN denotes in
T10723 3947-3950 DT denotes the
T10724 3951-3957 NN denotes mutant
T10725 3957-3959 , denotes ,
T10726 3959-3962 CC denotes but
T10727 3963-3970 JJ denotes overall
T10728 3971-3978 JJ denotes similar
T10730 3979-3983 NN denotes body
T10729 3984-3988 NN denotes size
T10731 3988-3989 . denotes .
T10732 3989-4025 sentence denotes (B) Shown are 23-d-old littermates.
T10733 3990-3991 -LRB- denotes (
T10734 3991-3992 LS denotes B
T10736 3992-3993 -RRB- denotes )
T10737 3994-3999 VBN denotes Shown
T10735 4000-4003 VBP denotes are
T10738 4004-4006 CD denotes 23
T10740 4006-4007 HYPH denotes -
T10739 4007-4008 NN denotes d
T10742 4008-4009 HYPH denotes -
T10741 4009-4012 JJ denotes old
T10743 4013-4024 NNS denotes littermates
T10744 4024-4025 . denotes .
T10745 4025-4115 sentence denotes The mutant is smaller in this case, but the tail is not as truncated as the mouse in (A).
T10746 4026-4029 DT denotes The
T10747 4030-4036 NN denotes mutant
T10748 4037-4039 VBZ denotes is
T10749 4040-4047 JJR denotes smaller
T10750 4048-4050 IN denotes in
T10751 4051-4055 DT denotes this
T10752 4056-4060 NN denotes case
T10753 4060-4062 , denotes ,
T10754 4062-4065 CC denotes but
T10755 4066-4069 DT denotes the
T10756 4070-4074 NN denotes tail
T10757 4075-4077 VBZ denotes is
T10758 4078-4081 RB denotes not
T10759 4082-4084 RB denotes as
T10760 4085-4094 JJ denotes truncated
T10761 4095-4097 IN denotes as
T10762 4098-4101 DT denotes the
T10763 4102-4107 NN denotes mouse
T10764 4108-4110 IN denotes in
T10765 4111-4112 -LRB- denotes (
T10766 4112-4113 NN denotes A
T10767 4113-4114 -RRB- denotes )
T10768 4114-4115 . denotes .
T10769 4115-4177 sentence denotes (C) Wild-type (WT) and homozygous Trip13 mutant (MUT) testes.
T10770 4116-4117 -LRB- denotes (
T10771 4117-4118 LS denotes C
T10773 4118-4119 -RRB- denotes )
T10774 4120-4124 JJ denotes Wild
T10776 4124-4125 HYPH denotes -
T10775 4125-4129 NN denotes type
T10777 4130-4131 -LRB- denotes (
T10778 4131-4133 NN denotes WT
T10779 4133-4134 -RRB- denotes )
T10780 4135-4138 CC denotes and
T10781 4139-4149 JJ denotes homozygous
T10783 4150-4156 NN denotes Trip13
T10782 4157-4163 NN denotes mutant
T10784 4164-4165 -LRB- denotes (
T10785 4165-4168 NN denotes MUT
T10786 4168-4169 -RRB- denotes )
T10772 4170-4176 NNS denotes testes
T10787 4176-4177 . denotes .
T10788 4177-4298 sentence denotes (D) and (E) are cross sections through 17.5-d-old heterozygous (“WT”) and homozygous mutant Trip13 testes, respectively.
T10789 4178-4179 -LRB- denotes (
T10790 4179-4180 NN denotes D
T10792 4180-4181 -RRB- denotes )
T10793 4182-4185 CC denotes and
T10794 4186-4187 -LRB- denotes (
T10795 4187-4188 NN denotes E
T10796 4188-4189 -RRB- denotes )
T10791 4190-4193 VBP denotes are
T10797 4194-4199 NN denotes cross
T10798 4200-4208 NNS denotes sections
T10799 4209-4216 IN denotes through
T10800 4217-4221 CD denotes 17.5
T10802 4221-4222 HYPH denotes -
T10801 4222-4223 NN denotes d
T10804 4223-4224 HYPH denotes -
T10803 4224-4227 JJ denotes old
T10806 4228-4240 JJ denotes heterozygous
T10808 4241-4242 -LRB- denotes (
T10810 4242-4243 `` denotes “
T10809 4243-4245 NN denotes WT
T10811 4245-4246 '' denotes ”
T10812 4246-4247 -RRB- denotes )
T10813 4248-4251 CC denotes and
T10814 4252-4262 JJ denotes homozygous
T10807 4263-4269 NN denotes mutant
T10815 4270-4276 NN denotes Trip13
T10805 4277-4283 NNS denotes testes
T10816 4283-4285 , denotes ,
T10817 4285-4297 RB denotes respectively
T10818 4297-4298 . denotes .
T10819 4298-4511 sentence denotes Whereas the tubules in WT show coordinated spermatogenesis with pachytene spermatocytes present in all tubules (proximal to the lumen), developmental progression in the mutant is not synchronized between tubules.
T10820 4299-4306 IN denotes Whereas
T10822 4307-4310 DT denotes the
T10823 4311-4318 NNS denotes tubules
T10824 4319-4321 IN denotes in
T10825 4322-4324 NN denotes WT
T10821 4325-4329 VBP denotes show
T10827 4330-4341 VBN denotes coordinated
T10828 4342-4357 NN denotes spermatogenesis
T10829 4358-4362 IN denotes with
T10830 4363-4372 NN denotes pachytene
T10831 4373-4386 NNS denotes spermatocytes
T10832 4387-4394 JJ denotes present
T10833 4395-4397 IN denotes in
T10834 4398-4401 DT denotes all
T10835 4402-4409 NNS denotes tubules
T10836 4410-4411 -LRB- denotes (
T10837 4411-4419 JJ denotes proximal
T10838 4420-4422 IN denotes to
T10839 4423-4426 DT denotes the
T10840 4427-4432 NN denotes lumen
T10841 4432-4433 -RRB- denotes )
T10842 4433-4435 , denotes ,
T10843 4435-4448 JJ denotes developmental
T10844 4449-4460 NN denotes progression
T10845 4461-4463 IN denotes in
T10846 4464-4467 DT denotes the
T10847 4468-4474 NN denotes mutant
T10848 4475-4477 VBZ denotes is
T10849 4478-4481 RB denotes not
T10826 4482-4494 VBN denotes synchronized
T10850 4495-4502 IN denotes between
T10851 4503-4510 NNS denotes tubules
T10852 4510-4511 . denotes .
T10853 4511-4627 sentence denotes Some tubules have no pachytene spermatocyes (asterisks), while in others, development is somewhat disorganized (#).
T10854 4512-4516 DT denotes Some
T10855 4517-4524 NNS denotes tubules
T10856 4525-4529 VBP denotes have
T10857 4530-4532 DT denotes no
T10859 4533-4542 NN denotes pachytene
T10858 4543-4555 NNS denotes spermatocyes
T10860 4556-4557 -LRB- denotes (
T10861 4557-4566 NNS denotes asterisks
T10862 4566-4567 -RRB- denotes )
T10863 4567-4569 , denotes ,
T10864 4569-4574 IN denotes while
T10866 4575-4577 IN denotes in
T10867 4578-4584 NNS denotes others
T10868 4584-4586 , denotes ,
T10869 4586-4597 NN denotes development
T10865 4598-4600 VBZ denotes is
T10870 4601-4609 RB denotes somewhat
T10871 4610-4622 JJ denotes disorganized
T10872 4623-4624 -LRB- denotes (
T10873 4624-4625 SYM denotes #
T10874 4625-4626 -RRB- denotes )
T10875 4626-4627 . denotes .
T1877 4628-4630 NN denotes RT
T1876 4628-4845 sentence denotes RT-PCR analysis of Trip13Gt expression (Figure 1D) revealed a low level of normally spliced transcripts in testes of homozygotes that is presumably a consequence of incomplete usage of the gene trap's splice acceptor.
T1879 4630-4631 HYPH denotes -
T1878 4631-4634 NN denotes PCR
T1880 4635-4643 NN denotes analysis
T1882 4644-4646 IN denotes of
T1883 4647-4655 NN denotes Trip13Gt
T1884 4656-4666 NN denotes expression
T1885 4667-4668 -LRB- denotes (
T1887 4668-4674 NN denotes Figure
T1886 4675-4677 NN denotes 1D
T1888 4677-4678 -RRB- denotes )
T1881 4679-4687 VBD denotes revealed
T1889 4688-4689 DT denotes a
T1891 4690-4693 JJ denotes low
T1890 4694-4699 NN denotes level
T1892 4700-4702 IN denotes of
T1893 4703-4711 RB denotes normally
T1894 4712-4719 VBN denotes spliced
T1895 4720-4731 NNS denotes transcripts
T1896 4732-4734 IN denotes in
T1897 4735-4741 NNS denotes testes
T1898 4742-4744 IN denotes of
T1899 4745-4756 NNS denotes homozygotes
T1900 4757-4761 WDT denotes that
T1901 4762-4764 VBZ denotes is
T1902 4765-4775 RB denotes presumably
T1903 4776-4777 DT denotes a
T1904 4778-4789 NN denotes consequence
T1905 4790-4792 IN denotes of
T1906 4793-4803 JJ denotes incomplete
T1907 4804-4809 NN denotes usage
T1908 4810-4812 IN denotes of
T1909 4813-4816 DT denotes the
T1911 4817-4821 NN denotes gene
T1910 4822-4826 NN denotes trap
T1913 4826-4828 POS denotes 's
T1914 4829-4835 NN denotes splice
T1912 4836-4844 NN denotes acceptor
T1915 4844-4845 . denotes .
T1916 4845-5073 sentence denotes Western blot analysis, using a polyclonal antibody raised against a peptide encoded by exon 3, revealed multiple species in wild-type and heterozygous testes, one of which corresponds to the expected size of 48 kDa (Figure 1E).
T1917 4846-4853 NNP denotes Western
T1918 4854-4858 NN denotes blot
T1919 4859-4867 NN denotes analysis
T1921 4867-4869 , denotes ,
T1922 4869-4874 VBG denotes using
T1923 4875-4876 DT denotes a
T1925 4877-4887 JJ denotes polyclonal
T1924 4888-4896 NN denotes antibody
T1926 4897-4903 VBN denotes raised
T1927 4904-4911 IN denotes against
T1928 4912-4913 DT denotes a
T1929 4914-4921 NN denotes peptide
T1930 4922-4929 VBN denotes encoded
T1931 4930-4932 IN denotes by
T1932 4933-4937 NN denotes exon
T1933 4938-4939 CD denotes 3
T1934 4939-4941 , denotes ,
T1920 4941-4949 VBD denotes revealed
T1935 4950-4958 JJ denotes multiple
T1936 4959-4966 NNS denotes species
T1937 4967-4969 IN denotes in
T1938 4970-4974 JJ denotes wild
T1940 4974-4975 HYPH denotes -
T1939 4975-4979 NN denotes type
T1942 4980-4983 CC denotes and
T1943 4984-4996 JJ denotes heterozygous
T1941 4997-5003 NNS denotes testes
T1944 5003-5005 , denotes ,
T1945 5005-5008 CD denotes one
T1947 5009-5011 IN denotes of
T1948 5012-5017 WDT denotes which
T1946 5018-5029 VBZ denotes corresponds
T1949 5030-5032 IN denotes to
T1950 5033-5036 DT denotes the
T1952 5037-5045 VBN denotes expected
T1951 5046-5050 NN denotes size
T1953 5051-5053 IN denotes of
T1954 5054-5056 CD denotes 48
T1955 5057-5060 NN denotes kDa
T1956 5061-5062 -LRB- denotes (
T1958 5062-5068 NN denotes Figure
T1957 5069-5071 NN denotes 1E
T1959 5071-5072 -RRB- denotes )
T1960 5072-5073 . denotes .
T1961 5073-5218 sentence denotes This and three other species were undetectable in homozygous mutant testes, but a reduced amount of an intense ∼38 kDa smaller band was present.
T1962 5074-5078 DT denotes This
T1964 5079-5082 CC denotes and
T1965 5083-5088 CD denotes three
T1967 5089-5094 JJ denotes other
T1966 5095-5102 NNS denotes species
T1963 5103-5107 VBD denotes were
T1968 5108-5120 JJ denotes undetectable
T1969 5121-5123 IN denotes in
T1970 5124-5134 JJ denotes homozygous
T1971 5135-5141 NN denotes mutant
T1972 5142-5148 NNS denotes testes
T1973 5148-5150 , denotes ,
T1974 5150-5153 CC denotes but
T1975 5154-5155 DT denotes a
T1977 5156-5163 VBN denotes reduced
T1976 5164-5170 NN denotes amount
T1979 5171-5173 IN denotes of
T1980 5174-5176 DT denotes an
T1982 5177-5184 JJ denotes intense
T1983 5185-5186 SYM denotes ∼
T1984 5186-5188 CD denotes 38
T1985 5189-5192 NN denotes kDa
T1986 5193-5200 JJR denotes smaller
T1981 5201-5205 NN denotes band
T1978 5206-5209 VBD denotes was
T1987 5210-5217 JJ denotes present
T1988 5217-5218 . denotes .
T1989 5218-5265 sentence denotes It is not clear if this corresponds to TRIP13.
T1990 5219-5221 PRP denotes It
T1991 5222-5224 VBZ denotes is
T1992 5225-5228 RB denotes not
T1993 5229-5234 JJ denotes clear
T1994 5235-5237 IN denotes if
T1996 5238-5242 DT denotes this
T1995 5243-5254 VBZ denotes corresponds
T1997 5255-5257 IN denotes to
T1998 5258-5264 NN denotes TRIP13
T1999 5264-5265 . denotes .
T2000 5265-5410 sentence denotes The greatly decreased Trip13 mRNA and predicted correct-length protein in mutants indicate that the Trip13RRB047 allele is severely hypomorphic.
T2001 5266-5269 DT denotes The
T2003 5270-5277 RB denotes greatly
T2004 5278-5287 VBN denotes decreased
T2005 5288-5294 NN denotes Trip13
T2002 5295-5299 NN denotes mRNA
T2007 5300-5303 CC denotes and
T2008 5304-5313 VBN denotes predicted
T2010 5314-5321 JJ denotes correct
T2012 5321-5322 HYPH denotes -
T2011 5322-5328 NN denotes length
T2009 5329-5336 NN denotes protein
T2013 5337-5339 IN denotes in
T2014 5340-5347 NNS denotes mutants
T2006 5348-5356 VBP denotes indicate
T2015 5357-5361 IN denotes that
T2017 5362-5365 DT denotes the
T2019 5366-5378 NN denotes Trip13RRB047
T2018 5379-5385 NN denotes allele
T2016 5386-5388 VBZ denotes is
T2020 5389-5397 RB denotes severely
T2021 5398-5409 JJ denotes hypomorphic
T2022 5409-5410 . denotes .
T2023 5410-5682 sentence denotes To determine the germ cell types in which TRIP13 is expressed, and to assess possible expression in the mutant by means other than Western analysis, testis sections were immunolabeled for TRIP13 using a polyclonal chicken antipeptide antibody (see Materials and Methods).
T2024 5411-5413 TO denotes To
T2025 5414-5423 VB denotes determine
T2027 5424-5427 DT denotes the
T2029 5428-5432 NN denotes germ
T2030 5433-5437 NN denotes cell
T2028 5438-5443 NNS denotes types
T2031 5444-5446 IN denotes in
T2033 5447-5452 WDT denotes which
T2034 5453-5459 NN denotes TRIP13
T2035 5460-5462 VBZ denotes is
T2032 5463-5472 VBN denotes expressed
T2036 5472-5474 , denotes ,
T2037 5474-5477 CC denotes and
T2038 5478-5480 TO denotes to
T2039 5481-5487 VB denotes assess
T2040 5488-5496 JJ denotes possible
T2041 5497-5507 NN denotes expression
T2042 5508-5510 IN denotes in
T2043 5511-5514 DT denotes the
T2044 5515-5521 NN denotes mutant
T2045 5522-5524 IN denotes by
T2046 5525-5530 NNS denotes means
T2047 5531-5536 JJ denotes other
T2048 5537-5541 IN denotes than
T2049 5542-5549 NNP denotes Western
T2050 5550-5558 NN denotes analysis
T2051 5558-5560 , denotes ,
T2052 5560-5566 NN denotes testis
T2053 5567-5575 NNS denotes sections
T2054 5576-5580 VBD denotes were
T2026 5581-5594 VBN denotes immunolabeled
T2055 5595-5598 IN denotes for
T2056 5599-5605 NN denotes TRIP13
T2057 5606-5611 VBG denotes using
T2058 5612-5613 DT denotes a
T2060 5614-5624 JJ denotes polyclonal
T2062 5625-5632 NN denotes chicken
T2061 5633-5644 NN denotes antipeptide
T2059 5645-5653 NN denotes antibody
T2063 5654-5655 -LRB- denotes (
T2064 5655-5658 VB denotes see
T2065 5659-5668 NNS denotes Materials
T2066 5669-5672 CC denotes and
T2067 5673-5680 NNS denotes Methods
T2068 5680-5681 -RRB- denotes )
T2069 5681-5682 . denotes .
T2070 5682-5800 sentence denotes The most intensely labeled cells in control testes were Type B spermatogonia and leptotene spermatocytes (Figure 1F).
T2071 5683-5686 DT denotes The
T2073 5687-5691 RBS denotes most
T2074 5692-5701 RB denotes intensely
T2075 5702-5709 VBN denotes labeled
T2072 5710-5715 NNS denotes cells
T2077 5716-5718 IN denotes in
T2078 5719-5726 NN denotes control
T2079 5727-5733 NNS denotes testes
T2076 5734-5738 VBD denotes were
T2080 5739-5743 NN denotes Type
T2081 5744-5745 NN denotes B
T2082 5746-5759 NNS denotes spermatogonia
T2083 5760-5763 CC denotes and
T2084 5764-5773 NN denotes leptotene
T2085 5774-5787 NNS denotes spermatocytes
T2086 5788-5789 -LRB- denotes (
T2088 5789-5795 NN denotes Figure
T2087 5796-5798 NN denotes 1F
T2089 5798-5799 -RRB- denotes )
T2090 5799-5800 . denotes .
T2091 5800-5926 sentence denotes Zygotene/pachytene spermatocytes stained less strongly, and there was no detectable staining in late pachytene spermatocytes.
T2092 5801-5809 NN denotes Zygotene
T2094 5809-5810 HYPH denotes /
T2093 5810-5819 NN denotes pachytene
T2095 5820-5833 NNS denotes spermatocytes
T2096 5834-5841 VBD denotes stained
T2097 5842-5846 RBR denotes less
T2098 5847-5855 RB denotes strongly
T2099 5855-5857 , denotes ,
T2100 5857-5860 CC denotes and
T2101 5861-5866 EX denotes there
T2102 5867-5870 VBD denotes was
T2103 5871-5873 DT denotes no
T2105 5874-5884 JJ denotes detectable
T2104 5885-5893 NN denotes staining
T2106 5894-5896 IN denotes in
T2107 5897-5901 JJ denotes late
T2108 5902-5911 NN denotes pachytene
T2109 5912-5925 NNS denotes spermatocytes
T2110 5925-5926 . denotes .
T2111 5926-5967 sentence denotes TRIP13 appeared to be nuclear localized.
T2112 5927-5933 NN denotes TRIP13
T2113 5934-5942 VBD denotes appeared
T2114 5943-5945 TO denotes to
T2115 5946-5948 VB denotes be
T2116 5949-5956 JJ denotes nuclear
T2117 5957-5966 VBN denotes localized
T2118 5966-5967 . denotes .
T2119 5967-6048 sentence denotes There was no such staining of nuclei in mutant seminiferous tubules (Figure 1F).
T2120 5968-5973 EX denotes There
T2121 5974-5977 VBD denotes was
T2122 5978-5980 DT denotes no
T2124 5981-5985 JJ denotes such
T2123 5986-5994 NN denotes staining
T2125 5995-5997 IN denotes of
T2126 5998-6004 NNS denotes nuclei
T2127 6005-6007 IN denotes in
T2128 6008-6014 NN denotes mutant
T2130 6015-6027 JJ denotes seminiferous
T2129 6028-6035 NNS denotes tubules
T2131 6036-6037 -LRB- denotes (
T2133 6037-6043 NN denotes Figure
T2132 6044-6046 NN denotes 1F
T2134 6046-6047 -RRB- denotes )
T2135 6047-6048 . denotes .
T2136 6048-6191 sentence denotes To further assess the nuclear localization, TRIP13 was used to probe meiotic chromosomes prepared by surface spreading of spermatocyte nuclei.
T2137 6049-6051 TO denotes To
T2139 6052-6059 RB denotes further
T2138 6060-6066 VB denotes assess
T2141 6067-6070 DT denotes the
T2143 6071-6078 JJ denotes nuclear
T2142 6079-6091 NN denotes localization
T2144 6091-6093 , denotes ,
T2145 6093-6099 NN denotes TRIP13
T2146 6100-6103 VBD denotes was
T2140 6104-6108 VBN denotes used
T2147 6109-6111 TO denotes to
T2148 6112-6117 VB denotes probe
T2149 6118-6125 JJ denotes meiotic
T2150 6126-6137 NNS denotes chromosomes
T2151 6138-6146 VBN denotes prepared
T2152 6147-6149 IN denotes by
T2153 6150-6157 NN denotes surface
T2154 6158-6167 NN denotes spreading
T2155 6168-6170 IN denotes of
T2156 6171-6183 NN denotes spermatocyte
T2157 6184-6190 NNS denotes nuclei
T2158 6190-6191 . denotes .
T2159 6191-6367 sentence denotes In wild type, there was diffuse nuclear staining, and no evidence of concentration on SC cores (marked by the axial element protein SYCP3) at any meiotic substage (Figure 1G).
T2160 6192-6194 IN denotes In
T2162 6195-6199 JJ denotes wild
T2163 6200-6204 NN denotes type
T2164 6204-6206 , denotes ,
T2165 6206-6211 EX denotes there
T2161 6212-6215 VBD denotes was
T2166 6216-6223 JJ denotes diffuse
T2168 6224-6231 JJ denotes nuclear
T2167 6232-6240 NN denotes staining
T2169 6240-6242 , denotes ,
T2170 6242-6245 CC denotes and
T2171 6246-6248 DT denotes no
T2172 6249-6257 NN denotes evidence
T2173 6258-6260 IN denotes of
T2174 6261-6274 NN denotes concentration
T2175 6275-6277 IN denotes on
T2176 6278-6280 NN denotes SC
T2177 6281-6286 NNS denotes cores
T2178 6287-6288 -LRB- denotes (
T2179 6288-6294 VBN denotes marked
T2180 6295-6297 IN denotes by
T2181 6298-6301 DT denotes the
T2183 6302-6307 JJ denotes axial
T2184 6308-6315 NN denotes element
T2185 6316-6323 NN denotes protein
T2182 6324-6329 NN denotes SYCP3
T2186 6329-6330 -RRB- denotes )
T2187 6331-6333 IN denotes at
T2188 6334-6337 DT denotes any
T2190 6338-6345 JJ denotes meiotic
T2189 6346-6354 NN denotes substage
T2191 6355-6356 -LRB- denotes (
T2193 6356-6362 NN denotes Figure
T2192 6363-6365 NN denotes 1G
T2194 6365-6366 -RRB- denotes )
T2195 6366-6367 . denotes .
T2196 6367-6429 sentence denotes TRIP13 signal was noticeably absent in mutant meiotic nuclei.
T2197 6368-6374 NN denotes TRIP13
T2198 6375-6381 NN denotes signal
T2199 6382-6385 VBD denotes was
T2200 6386-6396 RB denotes noticeably
T2201 6397-6403 JJ denotes absent
T2202 6404-6406 IN denotes in
T2203 6407-6413 NN denotes mutant
T2205 6414-6421 JJ denotes meiotic
T2204 6422-6428 NNS denotes nuclei
T2206 6428-6429 . denotes .
T2304 6431-6442 NN denotes Infertility
T2305 6443-6446 IN denotes Due
T2306 6447-6449 IN denotes to
T2307 6450-6457 JJ denotes Meiotic
T2308 6458-6468 NN denotes Disruption
T2309 6469-6471 IN denotes in
T2310 6472-6478 NN denotes TRIP13
T2312 6478-6479 HYPH denotes -
T2311 6479-6488 JJ denotes Deficient
T2313 6489-6498 NNS denotes Meiocytes
T2314 6498-6593 sentence denotes Homozygotes of both sexes had small gonads (Figure 2C; see below) and were invariably sterile.
T2315 6499-6510 NNS denotes Homozygotes
T2317 6511-6513 IN denotes of
T2318 6514-6518 DT denotes both
T2319 6519-6524 NNS denotes sexes
T2316 6525-6528 VBD denotes had
T2320 6529-6534 JJ denotes small
T2321 6535-6541 NNS denotes gonads
T2322 6542-6543 -LRB- denotes (
T2324 6543-6549 NN denotes Figure
T2323 6550-6552 NN denotes 2C
T2325 6552-6553 : denotes ;
T2326 6554-6557 VB denotes see
T2327 6558-6563 RB denotes below
T2328 6563-6564 -RRB- denotes )
T2329 6565-6568 CC denotes and
T2330 6569-6573 VBD denotes were
T2331 6574-6584 RB denotes invariably
T2332 6585-6592 JJ denotes sterile
T2333 6592-6593 . denotes .
T2334 6593-6703 sentence denotes Ovaries of adult Trip13Gt/Gt females were severely dysmorphic and had few or no follicles (Figure 3A and 3B).
T2335 6594-6601 NNS denotes Ovaries
T2337 6602-6604 IN denotes of
T2338 6605-6610 JJ denotes adult
T2340 6611-6619 NN denotes Trip13Gt
T2342 6619-6620 HYPH denotes /
T2341 6620-6622 NN denotes Gt
T2339 6623-6630 NNS denotes females
T2336 6631-6635 VBD denotes were
T2343 6636-6644 RB denotes severely
T2344 6645-6655 JJ denotes dysmorphic
T2345 6656-6659 CC denotes and
T2346 6660-6663 VBD denotes had
T2347 6664-6667 JJ denotes few
T2349 6668-6670 CC denotes or
T2350 6671-6673 DT denotes no
T2348 6674-6683 NNS denotes follicles
T2351 6684-6685 -LRB- denotes (
T2353 6685-6691 NN denotes Figure
T2352 6692-6694 NN denotes 3A
T2354 6695-6698 CC denotes and
T2355 6699-6701 NN denotes 3B
T2356 6701-6702 -RRB- denotes )
T2357 6702-6703 . denotes .
T2358 6703-6955 sentence denotes The majority of oocyte loss occurred in late embryogenesis or early in postnatal development, since 2 d postpartum ovaries were markedly smaller than those of control littermates, and were lacking oocytes or newly forming follicles (Figure 3C and 3D).
T2359 6704-6707 DT denotes The
T2360 6708-6716 NN denotes majority
T2362 6717-6719 IN denotes of
T2363 6720-6726 NN denotes oocyte
T2364 6727-6731 NN denotes loss
T2361 6732-6740 VBD denotes occurred
T2365 6741-6743 IN denotes in
T2366 6744-6748 JJ denotes late
T2367 6749-6762 NN denotes embryogenesis
T2368 6763-6765 CC denotes or
T2369 6766-6771 RB denotes early
T2370 6772-6774 IN denotes in
T2371 6775-6784 JJ denotes postnatal
T2372 6785-6796 NN denotes development
T2373 6796-6798 , denotes ,
T2374 6798-6803 IN denotes since
T2376 6804-6805 CD denotes 2
T2377 6806-6807 NN denotes d
T2379 6808-6818 RB denotes postpartum
T2378 6819-6826 NNS denotes ovaries
T2375 6827-6831 VBD denotes were
T2380 6832-6840 RB denotes markedly
T2381 6841-6848 JJR denotes smaller
T2382 6849-6853 IN denotes than
T2383 6854-6859 DT denotes those
T2384 6860-6862 IN denotes of
T2385 6863-6870 NN denotes control
T2386 6871-6882 NNS denotes littermates
T2387 6882-6884 , denotes ,
T2388 6884-6887 CC denotes and
T2389 6888-6892 VBD denotes were
T2390 6893-6900 VBG denotes lacking
T2391 6901-6908 NNS denotes oocytes
T2392 6909-6911 CC denotes or
T2393 6912-6917 RB denotes newly
T2394 6918-6925 VBG denotes forming
T2395 6926-6935 NNS denotes follicles
T2396 6936-6937 -LRB- denotes (
T2398 6937-6943 NN denotes Figure
T2397 6944-6946 NN denotes 3C
T2399 6947-6950 CC denotes and
T2400 6951-6953 NN denotes 3D
T2401 6953-6954 -RRB- denotes )
T2402 6954-6955 . denotes .
T2403 6955-7021 sentence denotes Thus, oocytes failed to progress to the dictyate (resting) phase.
T2404 6956-6960 RB denotes Thus
T2406 6960-6962 , denotes ,
T2407 6962-6969 NNS denotes oocytes
T2405 6970-6976 VBD denotes failed
T2408 6977-6979 TO denotes to
T2409 6980-6988 VB denotes progress
T2410 6989-6991 IN denotes to
T2411 6992-6995 DT denotes the
T2413 6996-7004 NN denotes dictyate
T2414 7005-7006 -LRB- denotes (
T2415 7006-7013 VBG denotes resting
T2416 7013-7014 -RRB- denotes )
T2412 7015-7020 NN denotes phase
T2417 7020-7021 . denotes .
T2418 7021-7226 sentence denotes Since we observed oocytes with pachytene stage chromosomes in 17.5 d Trip13Gt/Gt embryonic ovaries (unpublished data), this indicates that oocytes were eliminated somewhere between pachynema and dictyate.
T2419 7022-7027 IN denotes Since
T2421 7028-7030 PRP denotes we
T2420 7031-7039 VBD denotes observed
T2423 7040-7047 NNS denotes oocytes
T2424 7048-7052 IN denotes with
T2425 7053-7062 NN denotes pachytene
T2426 7063-7068 NN denotes stage
T2427 7069-7080 NNS denotes chromosomes
T2428 7081-7083 IN denotes in
T2429 7084-7088 CD denotes 17.5
T2430 7089-7090 NN denotes d
T2432 7091-7099 NN denotes Trip13Gt
T2434 7099-7100 HYPH denotes /
T2433 7100-7102 NN denotes Gt
T2435 7103-7112 JJ denotes embryonic
T2431 7113-7120 NNS denotes ovaries
T2436 7121-7122 -LRB- denotes (
T2438 7122-7133 JJ denotes unpublished
T2437 7134-7138 NNS denotes data
T2439 7138-7139 -RRB- denotes )
T2440 7139-7141 , denotes ,
T2441 7141-7145 DT denotes this
T2422 7146-7155 VBZ denotes indicates
T2442 7156-7160 IN denotes that
T2444 7161-7168 NNS denotes oocytes
T2445 7169-7173 VBD denotes were
T2443 7174-7184 VBN denotes eliminated
T2446 7185-7194 RB denotes somewhere
T2447 7195-7202 IN denotes between
T2448 7203-7212 NN denotes pachynema
T2449 7213-7216 CC denotes and
T2450 7217-7225 NN denotes dictyate
T2451 7225-7226 . denotes .
T2452 7226-9120 sentence denotes Figure 3 Histology of Mutant Gonads All are hematoxylin/eosin-stained paraffin sections. Testes are from 6-wk-old males, except as indicated below. (A) Wild-type 25-d-old ovary. (B) Trip13Gt /Gt 25-d-old ovary, showing dysgenesis from an absence of oocytes. (C) Trip13Gt/+ 2-d-old control ovary. Arrows point to oocytes in newly forming follicles. (D) Trip13Gt/Gt 2-d-old ovary, dysgenic due to lack of oocytes. Magnification is the same as its littermate in “C.” (E) Wild-type testis. (F) Trip13Gt/Gt testis with uniform pachytene arrest. (G) Trip13Gt/Gt 3-mo-old testis with some postmeiotic spermatids (arrows). (H) Spo11−/- testis. A tubule with spermatocytes at leptotene/zygotene transition is labeled ZP, and tubules with apoptotic spermatocytes are marked with an asterisk. The specimen was taken from a littermate of that in (I). (I) Spo11 −/− Trip13Gt/Gt testis. Labeling is the same as in (H). The inset contains a tubule with leptotene-zygotene spermatocytes. (J) Mei1 −/− Trip13Gt/+ testis. The specimen was taken from a littermate of that in (K). (K) Mei1 −/− Trip13Gt/Gt testis. (L) Rec8Mei8/Rec8Mei8 Trip13Gt/+ testis. The Rec8Mei8 allele was described [39]. The specimen was taken from a littermate of that in (M). (M) Rec8Mei8/Rec8Mei8 Trip13Gt/Gt testis. (N) Dmc1 −/− Trip13Gt/Gt testis. (O) Spo11 −/− Trip13Gt/+ 25-d-old ovary. The specimen was taken from a littermate of that in (P). (P) Spo11 −/− Trip13Gt/Gt 25-d-old ovary. (Q) Mei1 −/− Trip13Gt/+ 25-d-old ovary. The specimen was taken from a littermate of that in (R). (R) Mei1 −/− Trip13Gt/Gt 25-d-old ovary. (S) Rec8Mei8/Rec8Mei8 Trip13Gt/+ 25-d-old ovary. The specimen was taken from a littermate of that in (T). (T) Rec8Mei8/Rec8Mei8 Trip13Gt/Gt 25-d-old ovary. Histological sections of mutant testes revealed a lack of postmeiotic cell types that are characteristic of wild-type seminiferous tubules (Figure 3E).
T11057 7237-7246 NN denotes Histology
T11058 7247-7249 IN denotes of
T11059 7250-7256 NN denotes Mutant
T11060 7257-7263 NNS denotes Gonads
T11061 7263-7316 sentence denotes All are hematoxylin/eosin-stained paraffin sections.
T11062 7264-7267 DT denotes All
T11063 7268-7271 VBP denotes are
T11064 7272-7283 NN denotes hematoxylin
T11066 7283-7284 HYPH denotes /
T11065 7284-7289 NN denotes eosin
T11068 7289-7290 HYPH denotes -
T11067 7290-7297 VBN denotes stained
T11070 7298-7306 NN denotes paraffin
T11069 7307-7315 NNS denotes sections
T11071 7315-7316 . denotes .
T11072 7316-7375 sentence denotes Testes are from 6-wk-old males, except as indicated below.
T11073 7317-7323 NNS denotes Testes
T11074 7324-7327 VBP denotes are
T11075 7328-7332 IN denotes from
T11076 7333-7334 CD denotes 6
T11078 7334-7335 HYPH denotes -
T11077 7335-7337 NN denotes wk
T11080 7337-7338 HYPH denotes -
T11079 7338-7341 JJ denotes old
T11081 7342-7347 NNS denotes males
T11082 7347-7349 , denotes ,
T11083 7349-7355 IN denotes except
T11084 7356-7358 IN denotes as
T11085 7359-7368 VBN denotes indicated
T11086 7369-7374 RB denotes below
T11087 7374-7375 . denotes .
T11088 7375-7405 sentence denotes (A) Wild-type 25-d-old ovary.
T11089 7376-7377 -LRB- denotes (
T11090 7377-7378 LS denotes A
T11092 7378-7379 -RRB- denotes )
T11093 7380-7384 JJ denotes Wild
T11095 7384-7385 HYPH denotes -
T11094 7385-7389 NN denotes type
T11096 7390-7392 CD denotes 25
T11098 7392-7393 HYPH denotes -
T11097 7393-7394 NN denotes d
T11100 7394-7395 HYPH denotes -
T11099 7395-7398 JJ denotes old
T11091 7399-7404 NN denotes ovary
T11101 7404-7405 . denotes .
T11102 7405-7485 sentence denotes (B) Trip13Gt /Gt 25-d-old ovary, showing dysgenesis from an absence of oocytes.
T11103 7406-7407 -LRB- denotes (
T11104 7407-7408 LS denotes B
T11106 7408-7409 -RRB- denotes )
T11107 7410-7418 NN denotes Trip13Gt
T11109 7419-7420 HYPH denotes /
T11108 7420-7422 NN denotes Gt
T11110 7423-7425 CD denotes 25
T11112 7425-7426 HYPH denotes -
T11111 7426-7427 NN denotes d
T11114 7427-7428 HYPH denotes -
T11113 7428-7431 JJ denotes old
T11105 7432-7437 NN denotes ovary
T11115 7437-7439 , denotes ,
T11116 7439-7446 VBG denotes showing
T11117 7447-7457 NN denotes dysgenesis
T11118 7458-7462 IN denotes from
T11119 7463-7465 DT denotes an
T11120 7466-7473 NN denotes absence
T11121 7474-7476 IN denotes of
T11122 7477-7484 NNS denotes oocytes
T11123 7484-7485 . denotes .
T11124 7485-7523 sentence denotes (C) Trip13Gt/+ 2-d-old control ovary.
T11125 7486-7487 -LRB- denotes (
T11126 7487-7488 LS denotes C
T11128 7488-7489 -RRB- denotes )
T11129 7490-7498 NN denotes Trip13Gt
T11130 7498-7499 HYPH denotes /
T11131 7499-7500 SYM denotes +
T11132 7501-7502 CD denotes 2
T11134 7502-7503 HYPH denotes -
T11133 7503-7504 NN denotes d
T11136 7504-7505 HYPH denotes -
T11135 7505-7508 JJ denotes old
T11137 7509-7516 NN denotes control
T11127 7517-7522 NN denotes ovary
T11138 7522-7523 . denotes .
T11139 7523-7575 sentence denotes Arrows point to oocytes in newly forming follicles.
T11140 7524-7530 NNS denotes Arrows
T11141 7531-7536 VBP denotes point
T11142 7537-7539 IN denotes to
T11143 7540-7547 NNS denotes oocytes
T11144 7548-7550 IN denotes in
T11145 7551-7556 RB denotes newly
T11146 7557-7564 VBG denotes forming
T11147 7565-7574 NNS denotes follicles
T11148 7574-7575 . denotes .
T11149 7575-7639 sentence denotes (D) Trip13Gt/Gt 2-d-old ovary, dysgenic due to lack of oocytes.
T11150 7576-7577 -LRB- denotes (
T11151 7577-7578 LS denotes D
T11153 7578-7579 -RRB- denotes )
T11154 7580-7588 NN denotes Trip13Gt
T11156 7588-7589 HYPH denotes /
T11155 7589-7591 NN denotes Gt
T11157 7592-7593 CD denotes 2
T11159 7593-7594 HYPH denotes -
T11158 7594-7595 NN denotes d
T11161 7595-7596 HYPH denotes -
T11160 7596-7599 JJ denotes old
T11152 7600-7605 NN denotes ovary
T11162 7605-7607 , denotes ,
T11163 7607-7615 JJ denotes dysgenic
T11164 7616-7619 IN denotes due
T11165 7620-7622 IN denotes to
T11166 7623-7627 NN denotes lack
T11167 7628-7630 IN denotes of
T11168 7631-7638 NNS denotes oocytes
T11169 7638-7639 . denotes .
T11170 7639-7691 sentence denotes Magnification is the same as its littermate in “C.”
T11171 7640-7653 NN denotes Magnification
T11172 7654-7656 VBZ denotes is
T11173 7657-7660 DT denotes the
T11174 7661-7665 JJ denotes same
T11175 7666-7668 IN denotes as
T11176 7669-7672 PRP$ denotes its
T11177 7673-7683 NN denotes littermate
T11178 7684-7686 IN denotes in
T11179 7687-7688 `` denotes “
T11180 7688-7690 NN denotes C.
T11181 7690-7691 '' denotes ”
T11182 7691-7713 sentence denotes (E) Wild-type testis.
T11183 7692-7693 -LRB- denotes (
T11184 7693-7694 LS denotes E
T11186 7694-7695 -RRB- denotes )
T11187 7696-7700 JJ denotes Wild
T11189 7700-7701 HYPH denotes -
T11188 7701-7705 NN denotes type
T11185 7706-7712 NN denotes testis
T11190 7712-7713 . denotes .
T11191 7713-7767 sentence denotes (F) Trip13Gt/Gt testis with uniform pachytene arrest.
T11192 7714-7715 -LRB- denotes (
T11193 7715-7716 LS denotes F
T11195 7716-7717 -RRB- denotes )
T11196 7718-7726 NN denotes Trip13Gt
T11198 7726-7727 HYPH denotes /
T11197 7727-7729 NN denotes Gt
T11194 7730-7736 NN denotes testis
T11199 7737-7741 IN denotes with
T11200 7742-7749 JJ denotes uniform
T11202 7750-7759 NN denotes pachytene
T11201 7760-7766 NN denotes arrest
T11203 7766-7767 . denotes .
T11204 7767-7842 sentence denotes (G) Trip13Gt/Gt 3-mo-old testis with some postmeiotic spermatids (arrows).
T11205 7768-7769 -LRB- denotes (
T11206 7769-7770 LS denotes G
T11208 7770-7771 -RRB- denotes )
T11209 7772-7780 NN denotes Trip13Gt
T11211 7780-7781 HYPH denotes /
T11210 7781-7783 NN denotes Gt
T11212 7784-7785 CD denotes 3
T11214 7785-7786 HYPH denotes -
T11213 7786-7788 NN denotes mo
T11216 7788-7789 HYPH denotes -
T11215 7789-7792 JJ denotes old
T11207 7793-7799 NN denotes testis
T11217 7800-7804 IN denotes with
T11218 7805-7809 DT denotes some
T11220 7810-7821 JJ denotes postmeiotic
T11219 7822-7832 NNS denotes spermatids
T11221 7833-7834 -LRB- denotes (
T11222 7834-7840 NNS denotes arrows
T11223 7840-7841 -RRB- denotes )
T11224 7841-7842 . denotes .
T11225 7842-7863 sentence denotes (H) Spo11−/- testis.
T11226 7843-7844 -LRB- denotes (
T11227 7844-7845 LS denotes H
T11229 7845-7846 -RRB- denotes )
T11230 7847-7852 NN denotes Spo11
T11231 7852-7853 SYM denotes −
T11232 7853-7854 HYPH denotes /
T11233 7854-7855 SYM denotes -
T11228 7856-7862 NN denotes testis
T11234 7862-7863 . denotes .
T11235 7863-8009 sentence denotes A tubule with spermatocytes at leptotene/zygotene transition is labeled ZP, and tubules with apoptotic spermatocytes are marked with an asterisk.
T11236 7864-7865 DT denotes A
T11237 7866-7872 NN denotes tubule
T11239 7873-7877 IN denotes with
T11240 7878-7891 NNS denotes spermatocytes
T11241 7892-7894 IN denotes at
T11242 7895-7904 NN denotes leptotene
T11244 7904-7905 HYPH denotes /
T11243 7905-7913 NN denotes zygotene
T11245 7914-7924 NN denotes transition
T11246 7925-7927 VBZ denotes is
T11238 7928-7935 VBN denotes labeled
T11247 7936-7938 NN denotes ZP
T11248 7938-7940 , denotes ,
T11249 7940-7943 CC denotes and
T11250 7944-7951 NNS denotes tubules
T11252 7952-7956 IN denotes with
T11253 7957-7966 JJ denotes apoptotic
T11254 7967-7980 NNS denotes spermatocytes
T11255 7981-7984 VBP denotes are
T11251 7985-7991 VBN denotes marked
T11256 7992-7996 IN denotes with
T11257 7997-7999 DT denotes an
T11258 8000-8008 NN denotes asterisk
T11259 8008-8009 . denotes .
T11260 8009-8066 sentence denotes The specimen was taken from a littermate of that in (I).
T11261 8010-8013 DT denotes The
T11262 8014-8022 NN denotes specimen
T11264 8023-8026 VBD denotes was
T11263 8027-8032 VBN denotes taken
T11265 8033-8037 IN denotes from
T11266 8038-8039 DT denotes a
T11267 8040-8050 NN denotes littermate
T11268 8051-8053 IN denotes of
T11269 8054-8058 DT denotes that
T11270 8059-8061 IN denotes in
T11271 8062-8063 -LRB- denotes (
T11272 8063-8064 NN denotes I
T11273 8064-8065 -RRB- denotes )
T11274 8065-8066 . denotes .
T11275 8066-8100 sentence denotes (I) Spo11 −/− Trip13Gt/Gt testis.
T11276 8067-8068 -LRB- denotes (
T11277 8068-8069 LS denotes I
T11279 8069-8070 -RRB- denotes )
T11280 8071-8076 NN denotes Spo11
T11281 8077-8078 SYM denotes −
T11282 8078-8079 HYPH denotes /
T11283 8079-8080 SYM denotes −
T11284 8081-8089 NN denotes Trip13Gt
T11286 8089-8090 HYPH denotes /
T11285 8090-8092 NN denotes Gt
T11278 8093-8099 NN denotes testis
T11287 8099-8100 . denotes .
T11288 8100-8132 sentence denotes Labeling is the same as in (H).
T11289 8101-8109 NN denotes Labeling
T11290 8110-8112 VBZ denotes is
T11291 8113-8116 DT denotes the
T11292 8117-8121 JJ denotes same
T11293 8122-8124 IN denotes as
T11294 8125-8127 IN denotes in
T11295 8128-8129 -LRB- denotes (
T11296 8129-8130 NN denotes H
T11297 8130-8131 -RRB- denotes )
T11298 8131-8132 . denotes .
T11299 8132-8199 sentence denotes The inset contains a tubule with leptotene-zygotene spermatocytes.
T11300 8133-8136 DT denotes The
T11301 8137-8142 NN denotes inset
T11302 8143-8151 VBZ denotes contains
T11303 8152-8153 DT denotes a
T11304 8154-8160 NN denotes tubule
T11305 8161-8165 IN denotes with
T11306 8166-8175 NN denotes leptotene
T11308 8175-8176 HYPH denotes -
T11307 8176-8184 NN denotes zygotene
T11309 8185-8198 NNS denotes spermatocytes
T11310 8198-8199 . denotes .
T11311 8199-8231 sentence denotes (J) Mei1 −/− Trip13Gt/+ testis.
T11312 8200-8201 -LRB- denotes (
T11313 8201-8202 LS denotes J
T11315 8202-8203 -RRB- denotes )
T11316 8204-8208 NN denotes Mei1
T11317 8209-8210 SYM denotes −
T11318 8210-8211 HYPH denotes /
T11319 8211-8212 SYM denotes −
T11320 8213-8221 NN denotes Trip13Gt
T11321 8221-8222 HYPH denotes /
T11322 8222-8223 SYM denotes +
T11314 8224-8230 NN denotes testis
T11323 8230-8231 . denotes .
T11324 8231-8288 sentence denotes The specimen was taken from a littermate of that in (K).
T11325 8232-8235 DT denotes The
T11326 8236-8244 NN denotes specimen
T11328 8245-8248 VBD denotes was
T11327 8249-8254 VBN denotes taken
T11329 8255-8259 IN denotes from
T11330 8260-8261 DT denotes a
T11331 8262-8272 NN denotes littermate
T11332 8273-8275 IN denotes of
T11333 8276-8280 DT denotes that
T11334 8281-8283 IN denotes in
T11335 8284-8285 -LRB- denotes (
T11336 8285-8286 NN denotes K
T11337 8286-8287 -RRB- denotes )
T11338 8287-8288 . denotes .
T11339 8288-8321 sentence denotes (K) Mei1 −/− Trip13Gt/Gt testis.
T11340 8289-8290 -LRB- denotes (
T11341 8290-8291 LS denotes K
T11343 8291-8292 -RRB- denotes )
T11344 8293-8297 NN denotes Mei1
T11345 8298-8299 SYM denotes −
T11346 8299-8300 HYPH denotes /
T11347 8300-8301 SYM denotes −
T11348 8302-8310 NN denotes Trip13Gt
T11350 8310-8311 HYPH denotes /
T11349 8311-8313 NN denotes Gt
T11342 8314-8320 NN denotes testis
T11351 8320-8321 . denotes .
T11352 8321-8362 sentence denotes (L) Rec8Mei8/Rec8Mei8 Trip13Gt/+ testis.
T11353 8322-8323 -LRB- denotes (
T11354 8323-8324 LS denotes L
T11356 8324-8325 -RRB- denotes )
T11357 8326-8334 NN denotes Rec8Mei8
T11359 8334-8335 HYPH denotes /
T11358 8335-8343 NN denotes Rec8Mei8
T11360 8344-8352 NN denotes Trip13Gt
T11361 8352-8353 HYPH denotes /
T11362 8353-8354 SYM denotes +
T11355 8355-8361 NN denotes testis
T11363 8361-8362 . denotes .
T11364 8362-8402 sentence denotes The Rec8Mei8 allele was described [39].
T11365 8363-8366 DT denotes The
T11367 8367-8375 NN denotes Rec8Mei8
T11366 8376-8382 NN denotes allele
T11369 8383-8386 VBD denotes was
T11368 8387-8396 VBN denotes described
T11370 8397-8398 -LRB- denotes [
T11371 8398-8400 CD denotes 39
T11372 8400-8401 -RRB- denotes ]
T11373 8401-8402 . denotes .
T11374 8402-8459 sentence denotes The specimen was taken from a littermate of that in (M).
T11375 8403-8406 DT denotes The
T11376 8407-8415 NN denotes specimen
T11378 8416-8419 VBD denotes was
T11377 8420-8425 VBN denotes taken
T11379 8426-8430 IN denotes from
T11380 8431-8432 DT denotes a
T11381 8433-8443 NN denotes littermate
T11382 8444-8446 IN denotes of
T11383 8447-8451 DT denotes that
T11384 8452-8454 IN denotes in
T11385 8455-8456 -LRB- denotes (
T11386 8456-8457 NN denotes M
T11387 8457-8458 -RRB- denotes )
T11388 8458-8459 . denotes .
T11389 8459-8501 sentence denotes (M) Rec8Mei8/Rec8Mei8 Trip13Gt/Gt testis.
T11390 8460-8461 -LRB- denotes (
T11391 8461-8462 LS denotes M
T11393 8462-8463 -RRB- denotes )
T11394 8464-8472 NN denotes Rec8Mei8
T11396 8472-8473 HYPH denotes /
T11395 8473-8481 NN denotes Rec8Mei8
T11397 8482-8490 NN denotes Trip13Gt
T11399 8490-8491 HYPH denotes /
T11398 8491-8493 NN denotes Gt
T11392 8494-8500 NN denotes testis
T11400 8500-8501 . denotes .
T11401 8501-8534 sentence denotes (N) Dmc1 −/− Trip13Gt/Gt testis.
T11402 8502-8503 -LRB- denotes (
T11403 8503-8504 LS denotes N
T11405 8504-8505 -RRB- denotes )
T11406 8506-8510 NN denotes Dmc1
T11407 8511-8512 SYM denotes −
T11408 8512-8513 HYPH denotes /
T11409 8513-8514 SYM denotes −
T11410 8515-8523 NN denotes Trip13Gt
T11412 8523-8524 HYPH denotes /
T11411 8524-8526 NN denotes Gt
T11404 8527-8533 NN denotes testis
T11413 8533-8534 . denotes .
T11414 8534-8575 sentence denotes (O) Spo11 −/− Trip13Gt/+ 25-d-old ovary.
T11415 8535-8536 -LRB- denotes (
T11416 8536-8537 LS denotes O
T11418 8537-8538 -RRB- denotes )
T11419 8539-8544 NN denotes Spo11
T11420 8545-8546 SYM denotes −
T11421 8546-8547 HYPH denotes /
T11422 8547-8548 SYM denotes −
T11423 8549-8557 NN denotes Trip13Gt
T11424 8557-8558 HYPH denotes /
T11425 8558-8559 SYM denotes +
T11426 8560-8562 CD denotes 25
T11428 8562-8563 HYPH denotes -
T11427 8563-8564 NN denotes d
T11430 8564-8565 HYPH denotes -
T11429 8565-8568 JJ denotes old
T11417 8569-8574 NN denotes ovary
T11431 8574-8575 . denotes .
T11432 8575-8632 sentence denotes The specimen was taken from a littermate of that in (P).
T11433 8576-8579 DT denotes The
T11434 8580-8588 NN denotes specimen
T11436 8589-8592 VBD denotes was
T11435 8593-8598 VBN denotes taken
T11437 8599-8603 IN denotes from
T11438 8604-8605 DT denotes a
T11439 8606-8616 NN denotes littermate
T11440 8617-8619 IN denotes of
T11441 8620-8624 DT denotes that
T11442 8625-8627 IN denotes in
T11443 8628-8629 -LRB- denotes (
T11444 8629-8630 NN denotes P
T11445 8630-8631 -RRB- denotes )
T11446 8631-8632 . denotes .
T11447 8632-8674 sentence denotes (P) Spo11 −/− Trip13Gt/Gt 25-d-old ovary.
T11448 8633-8634 -LRB- denotes (
T11449 8634-8635 LS denotes P
T11451 8635-8636 -RRB- denotes )
T11452 8637-8642 NN denotes Spo11
T11453 8643-8644 SYM denotes −
T11454 8644-8645 HYPH denotes /
T11455 8645-8646 SYM denotes −
T11456 8647-8655 NN denotes Trip13Gt
T11458 8655-8656 HYPH denotes /
T11457 8656-8658 NN denotes Gt
T11459 8659-8661 CD denotes 25
T11461 8661-8662 HYPH denotes -
T11460 8662-8663 NN denotes d
T11463 8663-8664 HYPH denotes -
T11462 8664-8667 JJ denotes old
T11450 8668-8673 NN denotes ovary
T11464 8673-8674 . denotes .
T11465 8674-8714 sentence denotes (Q) Mei1 −/− Trip13Gt/+ 25-d-old ovary.
T11466 8675-8676 -LRB- denotes (
T11467 8676-8677 LS denotes Q
T11469 8677-8678 -RRB- denotes )
T11470 8679-8683 NN denotes Mei1
T11471 8684-8685 SYM denotes −
T11472 8685-8686 HYPH denotes /
T11473 8686-8687 SYM denotes −
T11474 8688-8696 NN denotes Trip13Gt
T11475 8696-8697 HYPH denotes /
T11476 8697-8698 SYM denotes +
T11477 8699-8701 CD denotes 25
T11479 8701-8702 HYPH denotes -
T11478 8702-8703 NN denotes d
T11481 8703-8704 HYPH denotes -
T11480 8704-8707 JJ denotes old
T11468 8708-8713 NN denotes ovary
T11482 8713-8714 . denotes .
T11483 8714-8771 sentence denotes The specimen was taken from a littermate of that in (R).
T11484 8715-8718 DT denotes The
T11485 8719-8727 NN denotes specimen
T11487 8728-8731 VBD denotes was
T11486 8732-8737 VBN denotes taken
T11488 8738-8742 IN denotes from
T11489 8743-8744 DT denotes a
T11490 8745-8755 NN denotes littermate
T11491 8756-8758 IN denotes of
T11492 8759-8763 DT denotes that
T11493 8764-8766 IN denotes in
T11494 8767-8768 -LRB- denotes (
T11495 8768-8769 NN denotes R
T11496 8769-8770 -RRB- denotes )
T11497 8770-8771 . denotes .
T11498 8771-8812 sentence denotes (R) Mei1 −/− Trip13Gt/Gt 25-d-old ovary.
T11499 8772-8773 -LRB- denotes (
T11500 8773-8774 LS denotes R
T11502 8774-8775 -RRB- denotes )
T11503 8776-8780 NN denotes Mei1
T11504 8781-8782 SYM denotes −
T11505 8782-8783 HYPH denotes /
T11506 8783-8784 SYM denotes −
T11507 8785-8793 NN denotes Trip13Gt
T11509 8793-8794 HYPH denotes /
T11508 8794-8796 NN denotes Gt
T11510 8797-8799 CD denotes 25
T11512 8799-8800 HYPH denotes -
T11511 8800-8801 NN denotes d
T11514 8801-8802 HYPH denotes -
T11513 8802-8805 JJ denotes old
T11501 8806-8811 NN denotes ovary
T11515 8811-8812 . denotes .
T11516 8812-8861 sentence denotes (S) Rec8Mei8/Rec8Mei8 Trip13Gt/+ 25-d-old ovary.
T11517 8813-8814 -LRB- denotes (
T11518 8814-8815 LS denotes S
T11520 8815-8816 -RRB- denotes )
T11521 8817-8825 NN denotes Rec8Mei8
T11523 8825-8826 HYPH denotes /
T11522 8826-8834 NN denotes Rec8Mei8
T11524 8835-8843 NN denotes Trip13Gt
T11525 8843-8844 HYPH denotes /
T11526 8844-8845 SYM denotes +
T11527 8846-8848 CD denotes 25
T11529 8848-8849 HYPH denotes -
T11528 8849-8850 NN denotes d
T11531 8850-8851 HYPH denotes -
T11530 8851-8854 JJ denotes old
T11519 8855-8860 NN denotes ovary
T11532 8860-8861 . denotes .
T11533 8861-8918 sentence denotes The specimen was taken from a littermate of that in (T).
T11534 8862-8865 DT denotes The
T11535 8866-8874 NN denotes specimen
T11537 8875-8878 VBD denotes was
T11536 8879-8884 VBN denotes taken
T11538 8885-8889 IN denotes from
T11539 8890-8891 DT denotes a
T11540 8892-8902 NN denotes littermate
T11541 8903-8905 IN denotes of
T11542 8906-8910 DT denotes that
T11543 8911-8913 IN denotes in
T11544 8914-8915 -LRB- denotes (
T11545 8915-8916 NN denotes T
T11546 8916-8917 -RRB- denotes )
T11547 8917-8918 . denotes .
T11548 8918-8968 sentence denotes (T) Rec8Mei8/Rec8Mei8 Trip13Gt/Gt 25-d-old ovary.
T11549 8919-8920 -LRB- denotes (
T11550 8920-8921 LS denotes T
T11552 8921-8922 -RRB- denotes )
T11553 8923-8931 NN denotes Rec8Mei8
T11555 8931-8932 HYPH denotes /
T11554 8932-8940 NN denotes Rec8Mei8
T11556 8941-8949 NN denotes Trip13Gt
T11558 8949-8950 HYPH denotes /
T11557 8950-8952 NN denotes Gt
T11559 8953-8955 CD denotes 25
T11561 8955-8956 HYPH denotes -
T11560 8956-8957 NN denotes d
T11563 8957-8958 HYPH denotes -
T11562 8958-8961 JJ denotes old
T11551 8962-8967 NN denotes ovary
T11564 8967-8968 . denotes .
T2453 8969-8981 JJ denotes Histological
T2454 8982-8990 NNS denotes sections
T2456 8991-8993 IN denotes of
T2457 8994-9000 NN denotes mutant
T2458 9001-9007 NNS denotes testes
T2455 9008-9016 VBD denotes revealed
T2459 9017-9018 DT denotes a
T2460 9019-9023 NN denotes lack
T2461 9024-9026 IN denotes of
T2462 9027-9038 JJ denotes postmeiotic
T2464 9039-9043 NN denotes cell
T2463 9044-9049 NNS denotes types
T2465 9050-9054 WDT denotes that
T2466 9055-9058 VBP denotes are
T2467 9059-9073 JJ denotes characteristic
T2468 9074-9076 IN denotes of
T2469 9077-9081 JJ denotes wild
T2471 9081-9082 HYPH denotes -
T2470 9082-9086 NN denotes type
T2473 9087-9099 JJ denotes seminiferous
T2472 9100-9107 NNS denotes tubules
T2474 9108-9109 -LRB- denotes (
T2476 9109-9115 NN denotes Figure
T2475 9116-9118 NN denotes 3E
T2477 9118-9119 -RRB- denotes )
T2478 9119-9120 . denotes .
T2479 9120-9275 sentence denotes The most developmentally advanced seminiferous tubules contained adluminal spermatocytes with condensed chromatin characteristic of pachynema (Figure 3F).
T2480 9121-9124 DT denotes The
T2482 9125-9129 RBS denotes most
T2484 9130-9145 RB denotes developmentally
T2483 9146-9154 VBN denotes advanced
T2485 9155-9167 JJ denotes seminiferous
T2481 9168-9175 NNS denotes tubules
T2486 9176-9185 VBD denotes contained
T2487 9186-9195 JJ denotes adluminal
T2488 9196-9209 NNS denotes spermatocytes
T2489 9210-9214 IN denotes with
T2490 9215-9224 VBN denotes condensed
T2491 9225-9234 NN denotes chromatin
T2492 9235-9249 JJ denotes characteristic
T2493 9250-9252 IN denotes of
T2494 9253-9262 NN denotes pachynema
T2495 9263-9264 -LRB- denotes (
T2497 9264-9270 NN denotes Figure
T2496 9271-9273 NN denotes 3F
T2498 9273-9274 -RRB- denotes )
T2499 9274-9275 . denotes .
T2500 9275-9383 sentence denotes The absence of coordinated spermatogenic progression beyond this stage is indicative of a pachytene arrest.
T2501 9276-9279 DT denotes The
T2502 9280-9287 NN denotes absence
T2504 9288-9290 IN denotes of
T2505 9291-9302 VBN denotes coordinated
T2507 9303-9316 JJ denotes spermatogenic
T2506 9317-9328 NN denotes progression
T2508 9329-9335 IN denotes beyond
T2509 9336-9340 DT denotes this
T2510 9341-9346 NN denotes stage
T2503 9347-9349 VBZ denotes is
T2511 9350-9360 JJ denotes indicative
T2512 9361-9363 IN denotes of
T2513 9364-9365 DT denotes a
T2515 9366-9375 NN denotes pachytene
T2514 9376-9382 NN denotes arrest
T2516 9382-9383 . denotes .
T2517 9383-9450 sentence denotes This was revealed more clearly by chromosome analysis (see below).
T2518 9384-9388 DT denotes This
T2520 9389-9392 VBD denotes was
T2519 9393-9401 VBN denotes revealed
T2521 9402-9406 RBR denotes more
T2522 9407-9414 RB denotes clearly
T2523 9415-9417 IN denotes by
T2524 9418-9428 NN denotes chromosome
T2525 9429-9437 NN denotes analysis
T2526 9438-9439 -LRB- denotes (
T2527 9439-9442 VB denotes see
T2528 9443-9448 RB denotes below
T2529 9448-9449 -RRB- denotes )
T2530 9449-9450 . denotes .
T2531 9450-9584 sentence denotes Some sections of adult seminiferous tubules contained postmeiotic spermatids (Figure 3G), although we saw no motile epididymal sperm.
T2532 9451-9455 DT denotes Some
T2533 9456-9464 NNS denotes sections
T2535 9465-9467 IN denotes of
T2536 9468-9473 JJ denotes adult
T2538 9474-9486 JJ denotes seminiferous
T2537 9487-9494 NNS denotes tubules
T2534 9495-9504 VBD denotes contained
T2539 9505-9516 JJ denotes postmeiotic
T2540 9517-9527 NNS denotes spermatids
T2541 9528-9529 -LRB- denotes (
T2543 9529-9535 NN denotes Figure
T2542 9536-9538 NN denotes 3G
T2544 9538-9539 -RRB- denotes )
T2545 9539-9541 , denotes ,
T2546 9541-9549 IN denotes although
T2548 9550-9552 PRP denotes we
T2547 9553-9556 VBD denotes saw
T2549 9557-9559 DT denotes no
T2551 9560-9566 JJ denotes motile
T2552 9567-9577 JJ denotes epididymal
T2550 9578-9583 NN denotes sperm
T2553 9583-9584 . denotes .
T2554 9584-9742 sentence denotes These drastic meiotic defects stand in contrast to yeast and C. elegans, in which deletion of Pch2 alone has minor effects on spore/gamete development [2,8].
T2555 9585-9590 DT denotes These
T2557 9591-9598 JJ denotes drastic
T2558 9599-9606 JJ denotes meiotic
T2556 9607-9614 NNS denotes defects
T2559 9615-9620 VBP denotes stand
T2560 9621-9623 IN denotes in
T2561 9624-9632 NN denotes contrast
T2562 9633-9635 IN denotes to
T2563 9636-9641 NN denotes yeast
T2564 9642-9645 CC denotes and
T2565 9646-9648 NNP denotes C.
T2566 9649-9656 NNP denotes elegans
T2567 9656-9658 , denotes ,
T2568 9658-9660 IN denotes in
T2570 9661-9666 WDT denotes which
T2571 9667-9675 NN denotes deletion
T2572 9676-9678 IN denotes of
T2573 9679-9683 NN denotes Pch2
T2574 9684-9689 RB denotes alone
T2569 9690-9693 VBZ denotes has
T2575 9694-9699 JJ denotes minor
T2576 9700-9707 NNS denotes effects
T2577 9708-9710 IN denotes on
T2578 9711-9716 NN denotes spore
T2580 9716-9717 HYPH denotes /
T2579 9717-9723 NN denotes gamete
T2581 9724-9735 NN denotes development
T2582 9736-9737 -LRB- denotes [
T2584 9737-9738 CD denotes 2
T2585 9738-9739 , denotes ,
T2583 9739-9740 CD denotes 8
T2586 9740-9741 -RRB- denotes ]
T2587 9741-9742 . denotes .
T2968 9744-9750 NN denotes TRIP13
T2970 9750-9751 HYPH denotes -
T2969 9751-9760 JJ denotes Deficient
T2971 9761-9770 NNS denotes Meiocytes
T2972 9771-9778 VBP denotes Undergo
T2973 9779-9789 JJ denotes Homologous
T2975 9790-9800 NN denotes Chromosome
T2974 9801-9809 NN denotes Synapsis
T2976 9810-9817 IN denotes Despite
T2977 9818-9821 DT denotes the
T2978 9822-9830 NN denotes Presence
T2979 9831-9833 IN denotes of
T2980 9834-9844 JJ denotes Unrepaired
T2981 9845-9849 NNS denotes DSBs
T2982 9850-9852 IN denotes in
T2983 9853-9862 NN denotes Pachynema
T2984 9862-10121 sentence denotes To better characterize the degree of meiotic progression in Trip13Gt/Gt spermatocytes, we immunostained chromosome spreads for SYCP3 and SYCP1, components of the axial/lateral elements and transverse filaments, respectively, of the synaptonemal complex (SC).
T2985 9863-9865 TO denotes To
T2987 9866-9872 RBR denotes better
T2986 9873-9885 VB denotes characterize
T2989 9886-9889 DT denotes the
T2990 9890-9896 NN denotes degree
T2991 9897-9899 IN denotes of
T2992 9900-9907 JJ denotes meiotic
T2993 9908-9919 NN denotes progression
T2994 9920-9922 IN denotes in
T2995 9923-9931 NN denotes Trip13Gt
T2997 9931-9932 HYPH denotes /
T2996 9932-9934 NN denotes Gt
T2998 9935-9948 NNS denotes spermatocytes
T2999 9948-9950 , denotes ,
T3000 9950-9952 PRP denotes we
T2988 9953-9966 VBD denotes immunostained
T3001 9967-9977 NN denotes chromosome
T3002 9978-9985 NNS denotes spreads
T3003 9986-9989 IN denotes for
T3004 9990-9995 NN denotes SYCP3
T3005 9996-9999 CC denotes and
T3006 10000-10005 NN denotes SYCP1
T3007 10005-10007 , denotes ,
T3008 10007-10017 NNS denotes components
T3009 10018-10020 IN denotes of
T3010 10021-10024 DT denotes the
T3012 10025-10030 JJ denotes axial
T3014 10030-10031 HYPH denotes /
T3013 10031-10038 JJ denotes lateral
T3011 10039-10047 NNS denotes elements
T3015 10048-10051 CC denotes and
T3016 10052-10062 JJ denotes transverse
T3017 10063-10072 NNS denotes filaments
T3018 10072-10074 , denotes ,
T3019 10074-10086 RB denotes respectively
T3020 10086-10088 , denotes ,
T3021 10088-10090 IN denotes of
T3022 10091-10094 DT denotes the
T3024 10095-10107 JJ denotes synaptonemal
T3023 10108-10115 NN denotes complex
T3025 10116-10117 -LRB- denotes (
T3026 10117-10119 NN denotes SC
T3027 10119-10120 -RRB- denotes )
T3028 10120-10121 . denotes .
T3029 10121-10353 sentence denotes Pachytene spermatocyte nuclei from postpubertal mutant testes could assemble normal SC cores and exhibited full synapsis of chromosomes as judged by colabeling of SYCP1 and SYCP3 along the full lengths of all autosomes (Figure 4A).
T3030 10122-10131 NN denotes Pachytene
T3032 10132-10144 NN denotes spermatocyte
T3031 10145-10151 NNS denotes nuclei
T3034 10152-10156 IN denotes from
T3035 10157-10169 JJ denotes postpubertal
T3037 10170-10176 NN denotes mutant
T3036 10177-10183 NNS denotes testes
T3038 10184-10189 MD denotes could
T3033 10190-10198 VB denotes assemble
T3039 10199-10205 JJ denotes normal
T3041 10206-10208 NN denotes SC
T3040 10209-10214 NNS denotes cores
T3042 10215-10218 CC denotes and
T3043 10219-10228 VBD denotes exhibited
T3044 10229-10233 JJ denotes full
T3045 10234-10242 NN denotes synapsis
T3046 10243-10245 IN denotes of
T3047 10246-10257 NNS denotes chromosomes
T3048 10258-10260 IN denotes as
T3049 10261-10267 VBN denotes judged
T3050 10268-10270 IN denotes by
T3051 10271-10281 VBG denotes colabeling
T3052 10282-10284 IN denotes of
T3053 10285-10290 NN denotes SYCP1
T3054 10291-10294 CC denotes and
T3055 10295-10300 NN denotes SYCP3
T3056 10301-10306 IN denotes along
T3057 10307-10310 DT denotes the
T3059 10311-10315 JJ denotes full
T3058 10316-10323 NNS denotes lengths
T3060 10324-10326 IN denotes of
T3061 10327-10330 DT denotes all
T3062 10331-10340 NNS denotes autosomes
T3063 10341-10342 -LRB- denotes (
T3065 10342-10348 NN denotes Figure
T3064 10349-10351 NN denotes 4A
T3066 10351-10352 -RRB- denotes )
T3067 10352-10353 . denotes .
T3068 10353-10447 sentence denotes Additionally, the X and Y chromosomes were normally synapsed at their pseudoautosomal region.
T3069 10354-10366 RB denotes Additionally
T3071 10366-10368 , denotes ,
T3072 10368-10371 DT denotes the
T3074 10372-10373 NN denotes X
T3075 10374-10377 CC denotes and
T3076 10378-10379 NN denotes Y
T3073 10380-10391 NNS denotes chromosomes
T3077 10392-10396 VBD denotes were
T3078 10397-10405 RB denotes normally
T3070 10406-10414 VBN denotes synapsed
T3079 10415-10417 IN denotes at
T3080 10418-10423 PRP$ denotes their
T3082 10424-10439 JJ denotes pseudoautosomal
T3081 10440-10446 NN denotes region
T3083 10446-10447 . denotes .
T3084 10447-10632 sentence denotes More prepubertal (17.5 d postpartum) mutant spermatocytes contained asynaptic or terminally asynapsed chromosomes than age-matched controls (62.5% versus 25%, respectively; Figure 4B).
T3085 10448-10452 RBR denotes More
T3087 10453-10464 JJ denotes prepubertal
T3088 10465-10466 -LRB- denotes (
T3089 10466-10470 CD denotes 17.5
T3090 10471-10472 NN denotes d
T3091 10473-10483 NN denotes postpartum
T3092 10483-10484 -RRB- denotes )
T3093 10485-10491 NN denotes mutant
T3086 10492-10505 NNS denotes spermatocytes
T3094 10506-10515 VBD denotes contained
T3095 10516-10525 JJ denotes asynaptic
T3097 10526-10528 CC denotes or
T3098 10529-10539 RB denotes terminally
T3099 10540-10549 VBN denotes asynapsed
T3096 10550-10561 NNS denotes chromosomes
T3100 10562-10566 IN denotes than
T3101 10567-10570 NN denotes age
T3103 10570-10571 HYPH denotes -
T3102 10571-10578 VBN denotes matched
T3104 10579-10587 NNS denotes controls
T3105 10588-10589 -LRB- denotes (
T3107 10589-10593 CD denotes 62.5
T3108 10593-10594 NN denotes %
T3109 10595-10601 CC denotes versus
T3110 10602-10604 CD denotes 25
T3111 10604-10605 NN denotes %
T3112 10605-10607 , denotes ,
T3113 10607-10619 RB denotes respectively
T3114 10619-10620 : denotes ;
T3115 10621-10627 NN denotes Figure
T3106 10628-10630 NN denotes 4B
T3116 10630-10631 -RRB- denotes )
T3117 10631-10632 . denotes .
T3118 10632-10801 sentence denotes We attribute this to a delay in the first wave of postnatal spermatogenesis (Figure 2D and 2E), likely related to systemic developmental retardation (Figure 2A and 2B).
T3119 10633-10635 PRP denotes We
T3120 10636-10645 VBP denotes attribute
T3121 10646-10650 DT denotes this
T3122 10651-10653 IN denotes to
T3123 10654-10655 DT denotes a
T3124 10656-10661 NN denotes delay
T3125 10662-10664 IN denotes in
T3126 10665-10668 DT denotes the
T3128 10669-10674 JJ denotes first
T3127 10675-10679 NN denotes wave
T3129 10680-10682 IN denotes of
T3130 10683-10692 JJ denotes postnatal
T3131 10693-10708 NN denotes spermatogenesis
T3132 10709-10710 -LRB- denotes (
T3134 10710-10716 NN denotes Figure
T3133 10717-10719 NN denotes 2D
T3135 10720-10723 CC denotes and
T3136 10724-10726 NN denotes 2E
T3137 10726-10727 -RRB- denotes )
T3138 10727-10729 , denotes ,
T3139 10729-10735 RB denotes likely
T3140 10736-10743 VBN denotes related
T3141 10744-10746 IN denotes to
T3142 10747-10755 JJ denotes systemic
T3144 10756-10769 JJ denotes developmental
T3143 10770-10781 NN denotes retardation
T3145 10782-10783 -LRB- denotes (
T3147 10783-10789 NN denotes Figure
T3146 10790-10792 NN denotes 2A
T3148 10793-10796 CC denotes and
T3149 10797-10799 NN denotes 2B
T3150 10799-10800 -RRB- denotes )
T3151 10800-10801 . denotes .
T3152 10801-11090 sentence denotes Nevertheless, since Trip13Gt/Gt spermatocytes progress to pachynema with no gross SC abnormalities, and oocytes were eliminated soon after birth (a characteristic of DNA repair mutants [13]), this suggested that unrepaired DSBs are responsible for eventual meiotic arrest and elimination.
T3153 10802-10814 RB denotes Nevertheless
T3155 10814-10816 , denotes ,
T3156 10816-10821 IN denotes since
T3158 10822-10830 NN denotes Trip13Gt
T3160 10830-10831 HYPH denotes /
T3159 10831-10833 NN denotes Gt
T3161 10834-10847 NNS denotes spermatocytes
T3157 10848-10856 VBP denotes progress
T3162 10857-10859 IN denotes to
T3163 10860-10869 NN denotes pachynema
T3164 10870-10874 IN denotes with
T3165 10875-10877 DT denotes no
T3167 10878-10883 JJ denotes gross
T3168 10884-10886 NN denotes SC
T3166 10887-10900 NNS denotes abnormalities
T3169 10900-10902 , denotes ,
T3170 10902-10905 CC denotes and
T3171 10906-10913 NNS denotes oocytes
T3173 10914-10918 VBD denotes were
T3172 10919-10929 VBN denotes eliminated
T3174 10930-10934 RB denotes soon
T3175 10935-10940 IN denotes after
T3176 10941-10946 NN denotes birth
T3177 10947-10948 -LRB- denotes (
T3179 10948-10949 DT denotes a
T3178 10950-10964 NN denotes characteristic
T3180 10965-10967 IN denotes of
T3181 10968-10971 NN denotes DNA
T3182 10972-10978 NN denotes repair
T3183 10979-10986 NNS denotes mutants
T3184 10987-10988 -LRB- denotes [
T3185 10988-10990 CD denotes 13
T3186 10990-10991 -RRB- denotes ]
T3187 10991-10992 -RRB- denotes )
T3188 10992-10994 , denotes ,
T3189 10994-10998 DT denotes this
T3154 10999-11008 VBD denotes suggested
T3190 11009-11013 IN denotes that
T3192 11014-11024 JJ denotes unrepaired
T3193 11025-11029 NNS denotes DSBs
T3191 11030-11033 VBP denotes are
T3194 11034-11045 JJ denotes responsible
T3195 11046-11049 IN denotes for
T3196 11050-11058 JJ denotes eventual
T3198 11059-11066 JJ denotes meiotic
T3197 11067-11073 NN denotes arrest
T3199 11074-11077 CC denotes and
T3200 11078-11089 NN denotes elimination
T3201 11089-11090 . denotes .
T3202 11090-11091 sentence denotes
T11701 11101-11120 JJ denotes Immunohistochemical
T11702 11121-11129 NN denotes Analysis
T11703 11130-11132 IN denotes of
T11704 11133-11142 NN denotes Pachytene
T11706 11143-11155 NN denotes Spermatocyte
T11705 11156-11167 NNS denotes Chromosomes
T11707 11167-11261 sentence denotes Surface-spread chromosomes were immunolabeled with the indicated antibodies and fluorophores.
T11708 11168-11175 NN denotes Surface
T11710 11175-11176 HYPH denotes -
T11709 11176-11182 NN denotes spread
T11711 11183-11194 NNS denotes chromosomes
T11713 11195-11199 VBD denotes were
T11712 11200-11213 VBN denotes immunolabeled
T11714 11214-11218 IN denotes with
T11715 11219-11222 DT denotes the
T11717 11223-11232 VBN denotes indicated
T11716 11233-11243 NNS denotes antibodies
T11718 11244-11247 CC denotes and
T11719 11248-11260 NNS denotes fluorophores
T11720 11260-11261 . denotes .
T11721 11261-11387 sentence denotes As indicated in the upper right of each panel, cells were from wild type (WT, either +/+ or Trip13Gt/+) or Trip13Gt/Gt (Mut).
T11722 11262-11264 IN denotes As
T11723 11265-11274 VBN denotes indicated
T11725 11275-11277 IN denotes in
T11726 11278-11281 DT denotes the
T11728 11282-11287 JJ denotes upper
T11727 11288-11293 NN denotes right
T11729 11294-11296 IN denotes of
T11730 11297-11301 DT denotes each
T11731 11302-11307 NN denotes panel
T11732 11307-11309 , denotes ,
T11733 11309-11314 NNS denotes cells
T11724 11315-11319 VBD denotes were
T11734 11320-11324 IN denotes from
T11735 11325-11329 JJ denotes wild
T11736 11330-11334 NN denotes type
T11737 11335-11336 -LRB- denotes (
T11738 11336-11338 NN denotes WT
T11739 11338-11340 , denotes ,
T11740 11340-11346 CC denotes either
T11742 11347-11348 SYM denotes +
T11743 11348-11349 HYPH denotes /
T11741 11349-11350 SYM denotes +
T11744 11351-11353 CC denotes or
T11745 11354-11362 NN denotes Trip13Gt
T11746 11362-11363 HYPH denotes /
T11747 11363-11364 SYM denotes +
T11748 11364-11365 -RRB- denotes )
T11749 11366-11368 CC denotes or
T11750 11369-11377 NN denotes Trip13Gt
T11752 11377-11378 HYPH denotes /
T11751 11378-11380 NN denotes Gt
T11753 11381-11382 -LRB- denotes (
T11754 11382-11385 NN denotes Mut
T11755 11385-11386 -RRB- denotes )
T11756 11386-11387 . denotes .
T11757 11387-11463 sentence denotes There were no differences seen between heterozygotes and +/+ spermatocytes.
T11758 11388-11393 EX denotes There
T11759 11394-11398 VBD denotes were
T11760 11399-11401 DT denotes no
T11761 11402-11413 NNS denotes differences
T11762 11414-11418 VBN denotes seen
T11763 11419-11426 IN denotes between
T11764 11427-11440 NNS denotes heterozygotes
T11765 11441-11444 CC denotes and
T11766 11445-11446 SYM denotes +
T11768 11446-11447 HYPH denotes /
T11767 11447-11448 SYM denotes +
T11769 11449-11462 NNS denotes spermatocytes
T11770 11462-11463 . denotes .
T11771 11463-11514 sentence denotes (A) A mutant pachytene nucleus with full synapsis.
T11772 11464-11465 -LRB- denotes (
T11773 11465-11466 LS denotes A
T11775 11466-11467 -RRB- denotes )
T11776 11468-11469 DT denotes A
T11777 11470-11476 NN denotes mutant
T11778 11477-11486 NN denotes pachytene
T11774 11487-11494 NN denotes nucleus
T11779 11495-11499 IN denotes with
T11780 11500-11504 JJ denotes full
T11781 11505-11513 NN denotes synapsis
T11782 11513-11514 . denotes .
T11783 11514-11558 sentence denotes Areas of SYCP1/SYCP3 colabeling are yellow.
T11784 11515-11520 NNS denotes Areas
T11786 11521-11523 IN denotes of
T11787 11524-11529 NN denotes SYCP1
T11789 11529-11530 HYPH denotes /
T11788 11530-11535 NN denotes SYCP3
T11790 11536-11546 NN denotes colabeling
T11785 11547-11550 VBP denotes are
T11791 11551-11557 JJ denotes yellow
T11792 11557-11558 . denotes .
T11793 11558-11617 sentence denotes (B–E) Spermatocytes nucleus from 17.5 d postpartum mutant.
T11794 11559-11560 -LRB- denotes (
T11795 11560-11561 LS denotes B
T11797 11561-11562 SYM denotes –
T11798 11562-11563 LS denotes E
T11799 11563-11564 -RRB- denotes )
T11800 11565-11578 NNS denotes Spermatocytes
T11796 11579-11586 NN denotes nucleus
T11801 11587-11591 IN denotes from
T11802 11592-11596 CD denotes 17.5
T11803 11597-11598 NN denotes d
T11804 11599-11609 JJ denotes postpartum
T11805 11610-11616 NN denotes mutant
T11806 11616-11617 . denotes .
T11807 11617-11721 sentence denotes Asynapsed chromosomes or regions of chromosomes are indicated by white and yellow arrows, respectively.
T11808 11618-11627 VBN denotes Asynapsed
T11809 11628-11639 NNS denotes chromosomes
T11811 11640-11642 CC denotes or
T11812 11643-11650 NNS denotes regions
T11813 11651-11653 IN denotes of
T11814 11654-11665 NNS denotes chromosomes
T11815 11666-11669 VBP denotes are
T11810 11670-11679 VBN denotes indicated
T11816 11680-11682 IN denotes by
T11817 11683-11688 JJ denotes white
T11819 11689-11692 CC denotes and
T11820 11693-11699 JJ denotes yellow
T11818 11700-11706 NNS denotes arrows
T11821 11706-11708 , denotes ,
T11822 11708-11720 RB denotes respectively
T11823 11720-11721 . denotes .
T11824 11721-11868 sentence denotes Unlike the normal distribution in wild-type pachytene spermatocytes (C), BLM foci are present on synapsed pachytene chromosomes in the mutant (D).
T11825 11722-11728 IN denotes Unlike
T11827 11729-11732 DT denotes the
T11829 11733-11739 JJ denotes normal
T11828 11740-11752 NN denotes distribution
T11830 11753-11755 IN denotes in
T11831 11756-11760 JJ denotes wild
T11833 11760-11761 HYPH denotes -
T11832 11761-11765 NN denotes type
T11835 11766-11775 NN denotes pachytene
T11834 11776-11789 NNS denotes spermatocytes
T11836 11790-11791 -LRB- denotes (
T11837 11791-11792 NN denotes C
T11838 11792-11793 -RRB- denotes )
T11839 11793-11795 , denotes ,
T11840 11795-11798 NN denotes BLM
T11841 11799-11803 NNS denotes foci
T11826 11804-11807 VBP denotes are
T11842 11808-11815 JJ denotes present
T11843 11816-11818 IN denotes on
T11844 11819-11827 VBN denotes synapsed
T11846 11828-11837 NN denotes pachytene
T11845 11838-11849 NNS denotes chromosomes
T11847 11850-11852 IN denotes in
T11848 11853-11856 DT denotes the
T11849 11857-11863 NN denotes mutant
T11850 11864-11865 -LRB- denotes (
T11851 11865-11866 NN denotes D
T11852 11866-11867 -RRB- denotes )
T11853 11867-11868 . denotes .
T11854 11868-12033 sentence denotes RAD51 foci, which are abundant earlier in prophase, disappear from autosomes in wild-type pachytene nuclei (E) and the bulk of staining is over the XY body (arrow).
T11855 11869-11874 NN denotes RAD51
T11856 11875-11879 NNS denotes foci
T11858 11879-11881 , denotes ,
T11859 11881-11886 WDT denotes which
T11860 11887-11890 VBP denotes are
T11861 11891-11899 JJ denotes abundant
T11862 11900-11907 RBR denotes earlier
T11863 11908-11910 IN denotes in
T11864 11911-11919 NN denotes prophase
T11865 11919-11921 , denotes ,
T11857 11921-11930 VBP denotes disappear
T11866 11931-11935 IN denotes from
T11867 11936-11945 NNS denotes autosomes
T11868 11946-11948 IN denotes in
T11869 11949-11953 JJ denotes wild
T11871 11953-11954 HYPH denotes -
T11870 11954-11958 NN denotes type
T11873 11959-11968 NN denotes pachytene
T11872 11969-11975 NNS denotes nuclei
T11874 11976-11977 -LRB- denotes (
T11875 11977-11978 NN denotes E
T11876 11978-11979 -RRB- denotes )
T11877 11980-11983 CC denotes and
T11878 11984-11987 DT denotes the
T11879 11988-11992 NN denotes bulk
T11881 11993-11995 IN denotes of
T11882 11996-12004 NN denotes staining
T11880 12005-12007 VBZ denotes is
T11883 12008-12012 IN denotes over
T11884 12013-12016 DT denotes the
T11886 12017-12019 NN denotes XY
T11885 12020-12024 NN denotes body
T11887 12025-12026 -LRB- denotes (
T11888 12026-12031 NN denotes arrow
T11889 12031-12032 -RRB- denotes )
T11890 12032-12033 . denotes .
T11891 12033-12097 sentence denotes (F) RAD51 persists on the synapsed mutant chromosomes (arrows).
T11892 12034-12035 -LRB- denotes (
T11893 12035-12036 LS denotes F
T11895 12036-12037 -RRB- denotes )
T11896 12038-12043 NN denotes RAD51
T11894 12044-12052 VBZ denotes persists
T11897 12053-12055 IN denotes on
T11898 12056-12059 DT denotes the
T11900 12060-12068 VBN denotes synapsed
T11901 12069-12075 NN denotes mutant
T11899 12076-12087 NNS denotes chromosomes
T11902 12088-12089 -LRB- denotes (
T11903 12089-12095 NNS denotes arrows
T11904 12095-12096 -RRB- denotes )
T11905 12096-12097 . denotes .
T11906 12097-12158 sentence denotes (G) H2AX phosphorylation is restricted to the XY body in WT.
T11907 12098-12099 -LRB- denotes (
T11908 12099-12100 LS denotes G
T11910 12100-12101 -RRB- denotes )
T11911 12102-12106 NN denotes H2AX
T11912 12107-12122 NN denotes phosphorylation
T11913 12123-12125 VBZ denotes is
T11909 12126-12136 VBN denotes restricted
T11914 12137-12139 IN denotes to
T11915 12140-12143 DT denotes the
T11917 12144-12146 NN denotes XY
T11916 12147-12151 NN denotes body
T11918 12152-12154 IN denotes in
T11919 12155-12157 NN denotes WT
T11920 12157-12158 . denotes .
T11921 12158-12294 sentence denotes (H) In addition to a large area of γH2AX staining (arrow) over the XY body, there is extensive autosomal H2AX phosphorylation (arrows).
T11922 12159-12160 -LRB- denotes (
T11923 12160-12161 LS denotes H
T11925 12161-12162 -RRB- denotes )
T11926 12163-12165 IN denotes In
T11927 12166-12174 NN denotes addition
T11928 12175-12177 IN denotes to
T11929 12178-12179 DT denotes a
T11931 12180-12185 JJ denotes large
T11930 12186-12190 NN denotes area
T11932 12191-12193 IN denotes of
T11933 12194-12199 NN denotes γH2AX
T11934 12200-12208 NN denotes staining
T11935 12209-12210 -LRB- denotes (
T11936 12210-12215 NN denotes arrow
T11937 12215-12216 -RRB- denotes )
T11938 12217-12221 IN denotes over
T11939 12222-12225 DT denotes the
T11941 12226-12228 NN denotes XY
T11940 12229-12233 NN denotes body
T11942 12233-12235 , denotes ,
T11943 12235-12240 EX denotes there
T11924 12241-12243 VBZ denotes is
T11944 12244-12253 JJ denotes extensive
T11946 12254-12263 JJ denotes autosomal
T11947 12264-12268 NN denotes H2AX
T11945 12269-12284 NN denotes phosphorylation
T11948 12285-12286 -LRB- denotes (
T11949 12286-12292 NNS denotes arrows
T11950 12292-12293 -RRB- denotes )
T11951 12293-12294 . denotes .
T11952 12294-12405 sentence denotes (I, J) Note that in wild-type pachytene spermatocytes, TOPBP1 is present only over the XY body (yellow arrow).
T11953 12295-12296 -LRB- denotes (
T11954 12296-12297 LS denotes I
T11956 12297-12299 , denotes ,
T11957 12299-12300 LS denotes J
T11958 12300-12301 -RRB- denotes )
T11955 12302-12306 VB denotes Note
T11959 12307-12311 IN denotes that
T11961 12312-12314 IN denotes in
T11962 12315-12319 JJ denotes wild
T11964 12319-12320 HYPH denotes -
T11963 12320-12324 NN denotes type
T11966 12325-12334 NN denotes pachytene
T11965 12335-12348 NNS denotes spermatocytes
T11967 12348-12350 , denotes ,
T11968 12350-12356 NN denotes TOPBP1
T11960 12357-12359 VBZ denotes is
T11969 12360-12367 JJ denotes present
T11970 12368-12372 RB denotes only
T11971 12373-12377 IN denotes over
T11972 12378-12381 DT denotes the
T11974 12382-12384 NN denotes XY
T11973 12385-12389 NN denotes body
T11975 12390-12391 -LRB- denotes (
T11977 12391-12397 JJ denotes yellow
T11976 12398-12403 NN denotes arrow
T11978 12403-12404 -RRB- denotes )
T11979 12404-12405 . denotes .
T11980 12405-12566 sentence denotes In the mutant (J), an arrow denotes one area of intensive staining that may be over the sex chromosomes, but many other chromosome cores are positively stained.
T11981 12406-12408 IN denotes In
T11983 12409-12412 DT denotes the
T11984 12413-12419 NN denotes mutant
T11985 12420-12421 -LRB- denotes (
T11986 12421-12422 NN denotes J
T11987 12422-12423 -RRB- denotes )
T11988 12423-12425 , denotes ,
T11989 12425-12427 DT denotes an
T11990 12428-12433 NN denotes arrow
T11982 12434-12441 VBZ denotes denotes
T11991 12442-12445 CD denotes one
T11992 12446-12450 NN denotes area
T11993 12451-12453 IN denotes of
T11994 12454-12463 JJ denotes intensive
T11995 12464-12472 NN denotes staining
T11996 12473-12477 WDT denotes that
T11998 12478-12481 MD denotes may
T11997 12482-12484 VB denotes be
T11999 12485-12489 IN denotes over
T12000 12490-12493 DT denotes the
T12002 12494-12497 NN denotes sex
T12001 12498-12509 NNS denotes chromosomes
T12003 12509-12511 , denotes ,
T12004 12511-12514 CC denotes but
T12005 12515-12519 JJ denotes many
T12007 12520-12525 JJ denotes other
T12008 12526-12536 NN denotes chromosome
T12006 12537-12542 NNS denotes cores
T12010 12543-12546 VBP denotes are
T12011 12547-12557 RB denotes positively
T12009 12558-12565 VBN denotes stained
T12012 12565-12566 . denotes .
T12013 12566-12630 sentence denotes (K, L) RPA persists along synapsed cores in the mutant, not WT.
T12014 12567-12568 -LRB- denotes (
T12015 12568-12569 LS denotes K
T12017 12569-12571 , denotes ,
T12018 12571-12572 LS denotes L
T12019 12572-12573 -RRB- denotes )
T12020 12574-12577 NN denotes RPA
T12016 12578-12586 VBZ denotes persists
T12021 12587-12592 IN denotes along
T12022 12593-12601 VBN denotes synapsed
T12023 12602-12607 NNS denotes cores
T12024 12608-12610 IN denotes in
T12025 12611-12614 DT denotes the
T12026 12615-12621 NN denotes mutant
T12027 12621-12623 , denotes ,
T12028 12623-12626 CC denotes not
T12029 12627-12629 NN denotes WT
T12030 12629-12630 . denotes .
T12031 12630-12683 sentence denotes (M, N) Arrows indicate examples of MLH3 foci on SCs.
T12032 12631-12632 -LRB- denotes (
T12033 12632-12633 LS denotes M
T12035 12633-12635 , denotes ,
T12036 12635-12636 LS denotes N
T12037 12636-12637 -RRB- denotes )
T12038 12638-12644 NNS denotes Arrows
T12034 12645-12653 VBP denotes indicate
T12039 12654-12662 NNS denotes examples
T12040 12663-12665 IN denotes of
T12041 12666-12670 NN denotes MLH3
T12042 12671-12675 NNS denotes foci
T12043 12676-12678 IN denotes on
T12044 12679-12682 NNS denotes SCs
T12045 12682-12683 . denotes .
T12046 12683-12767 sentence denotes (O) In WT late pachytene spermatocytes, RAD51 is present only at background levels.
T12047 12684-12685 -LRB- denotes (
T12048 12685-12686 LS denotes O
T12050 12686-12687 -RRB- denotes )
T12051 12688-12690 IN denotes In
T12052 12691-12693 NN denotes WT
T12054 12694-12698 JJ denotes late
T12055 12699-12708 NN denotes pachytene
T12053 12709-12722 NNS denotes spermatocytes
T12056 12722-12724 , denotes ,
T12057 12724-12729 NN denotes RAD51
T12049 12730-12732 VBZ denotes is
T12058 12733-12740 JJ denotes present
T12059 12741-12745 RB denotes only
T12060 12746-12748 IN denotes at
T12061 12749-12759 NN denotes background
T12062 12760-12766 NNS denotes levels
T12063 12766-12767 . denotes .
T12064 12767-12876 sentence denotes (P) As in (F), extensive RAD51 staining delineates SCs in mutant pachytene nuclei (indicated by white arcs).
T12065 12768-12769 -LRB- denotes (
T12066 12769-12770 LS denotes P
T12068 12770-12771 -RRB- denotes )
T12069 12772-12774 IN denotes As
T12070 12775-12777 IN denotes in
T12071 12778-12779 -LRB- denotes (
T12072 12779-12780 NN denotes F
T12073 12780-12781 -RRB- denotes )
T12074 12781-12783 , denotes ,
T12075 12783-12792 JJ denotes extensive
T12077 12793-12798 NN denotes RAD51
T12076 12799-12807 NN denotes staining
T12067 12808-12818 VBZ denotes delineates
T12078 12819-12822 NNS denotes SCs
T12079 12823-12825 IN denotes in
T12080 12826-12832 NN denotes mutant
T12082 12833-12842 NN denotes pachytene
T12081 12843-12849 NNS denotes nuclei
T12083 12850-12851 -LRB- denotes (
T12084 12851-12860 VBN denotes indicated
T12085 12861-12863 IN denotes by
T12086 12864-12869 JJ denotes white
T12087 12870-12874 NNS denotes arcs
T12088 12874-12875 -RRB- denotes )
T12089 12875-12876 . denotes .
T12090 12876-12974 sentence denotes MLH1 foci colocalize with these tracts (arrows) at the typical 1–2 foci per chromosome as in (M).
T12091 12877-12881 NN denotes MLH1
T12092 12882-12886 NNS denotes foci
T12093 12887-12897 VBP denotes colocalize
T12094 12898-12902 IN denotes with
T12095 12903-12908 DT denotes these
T12096 12909-12915 NNS denotes tracts
T12097 12916-12917 -LRB- denotes (
T12098 12917-12923 NNS denotes arrows
T12099 12923-12924 -RRB- denotes )
T12100 12925-12927 IN denotes at
T12101 12928-12931 DT denotes the
T12103 12932-12939 JJ denotes typical
T12104 12940-12941 CD denotes 1
T12106 12941-12942 SYM denotes –
T12105 12942-12943 CD denotes 2
T12102 12944-12948 NNS denotes foci
T12107 12949-12952 IN denotes per
T12108 12953-12963 NN denotes chromosome
T12109 12964-12966 IN denotes as
T12110 12967-12969 IN denotes in
T12111 12970-12971 -LRB- denotes (
T12112 12971-12972 NN denotes M
T12113 12972-12973 -RRB- denotes )
T12114 12973-12974 . denotes .
T3204 12975-12976 NN denotes T
T3203 12975-12978 sentence denotes To
T3205 12976-12977 CC denotes o
T3206 12978-12979 sentence denotes e
T3207 12978-12979 EX denotes e
T3208 12979-12981 sentence denotes lu
T3209 12979-12981 NN denotes lu
T3210 12981-12983 sentence denotes ci
T3211 12981-12983 NN denotes ci
T3213 12983-12984 NN denotes d
T3212 12983-12986 sentence denotes dat
T3214 12984-12986 JJ denotes at
T3216 12986-12987 VBN denotes e
T3215 12986-12993 sentence denotes e the c
T3217 12988-12989 VBN denotes t
T3218 12989-12991 JJ denotes he
T3219 12992-12993 RB denotes c
T3222 12993-12994 JJ denotes a
T3220 12993-12999 sentence denotes ause o
T3221 12994-12995 NN denotes u
T3223 12995-12997 NNS denotes se
T3224 12998-12999 NNS denotes o
T3226 12999-13000 NNS denotes f
T3225 12999-13007 sentence denotes f meioti
T3227 13000-13001 , denotes
T3228 13001-13002 IN denotes m
T3229 13002-13003 NNS denotes e
T3230 13003-13004 IN denotes i
T3231 13004-13005 IN denotes o
T3232 13005-13007 NN denotes ti
T3234 13007-13008 NNS denotes c
T3233 13007-13012 sentence denotes c arr
T3235 13009-13012 NNS denotes arr
T3236 13012-13015 sentence denotes est
T3237 13012-13015 VBN denotes est
T3239 13015-13017 , denotes ,
T3238 13015-13018 sentence denotes , w
T3240 13017-13018 NNS denotes w
T3242 13018-13019 VB denotes e
T3241 13018-13025 sentence denotes e analy
T3243 13020-13021 IN denotes a
T3244 13021-13022 IN denotes n
T3245 13022-13023 NN denotes a
T3246 13023-13025 RB denotes ly
T3247 13025-13027 sentence denotes ze
T3248 13025-13027 VBP denotes ze
T3250 13027-13028 VB denotes d
T3249 13027-13125 sentence denotes d meiotic chromosomes with a variety of markers that are diagnostic of recombination and synapsis.
T3251 13029-13036 JJ denotes meiotic
T3252 13037-13038 JJ denotes c
T3253 13038-13048 NNS denotes hromosomes
T3254 13049-13053 IN denotes with
T3255 13054-13055 DT denotes a
T3256 13056-13063 NN denotes variety
T3257 13064-13066 IN denotes of
T3258 13067-13074 NNS denotes markers
T3259 13075-13079 WDT denotes that
T3260 13080-13083 VBP denotes are
T3261 13084-13094 JJ denotes diagnostic
T3262 13095-13097 IN denotes of
T3263 13098-13111 NN denotes recombination
T3264 13112-13115 CC denotes and
T3265 13116-13124 NN denotes synapsis
T3266 13124-13125 . denotes .
T3267 13125-13330 sentence denotes Recombination in Trip13Gt/Gt spermatocytes appeared to initiate normally as judged by the presence of γH2AX in leptonema (Figure S2A and S2B), which reflects the presence of meiotically induced DSBs [18].
T3268 13126-13139 NN denotes Recombination
T3270 13140-13142 IN denotes in
T3271 13143-13151 NN denotes Trip13Gt
T3273 13151-13152 HYPH denotes /
T3272 13152-13154 NN denotes Gt
T3274 13155-13168 NNS denotes spermatocytes
T3269 13169-13177 VBD denotes appeared
T3275 13178-13180 TO denotes to
T3276 13181-13189 VB denotes initiate
T3277 13190-13198 RB denotes normally
T3278 13199-13201 IN denotes as
T3279 13202-13208 VBN denotes judged
T3280 13209-13211 IN denotes by
T3281 13212-13215 DT denotes the
T3282 13216-13224 NN denotes presence
T3283 13225-13227 IN denotes of
T3284 13228-13233 NN denotes γH2AX
T3285 13234-13236 IN denotes in
T3286 13237-13246 NN denotes leptonema
T3287 13247-13248 -LRB- denotes (
T3289 13248-13254 NN denotes Figure
T3288 13255-13258 NN denotes S2A
T3290 13259-13262 CC denotes and
T3291 13263-13266 NN denotes S2B
T3292 13266-13267 -RRB- denotes )
T3293 13267-13269 , denotes ,
T3294 13269-13274 WDT denotes which
T3295 13275-13283 VBZ denotes reflects
T3296 13284-13287 DT denotes the
T3297 13288-13296 NN denotes presence
T3298 13297-13299 IN denotes of
T3299 13300-13311 RB denotes meiotically
T3300 13312-13319 VBN denotes induced
T3301 13320-13324 NNS denotes DSBs
T3302 13325-13326 -LRB- denotes [
T3303 13326-13328 CD denotes 18
T3304 13328-13329 -RRB- denotes ]
T3305 13329-13330 . denotes .
T3306 13330-13600 sentence denotes RAD51 and/or DMC1, components of early recombination nodules (ERNs), was also present as abundant foci in Trip13Gt/Gt zygotene spermatocytes (unpublished data; the anti-RAD51 antibody cross-reacts with DMC1), indicating that recombinational repair of DSBs is initiated.
T3307 13331-13336 NN denotes RAD51
T3309 13337-13340 CC denotes and
T3310 13340-13341 HYPH denotes /
T3311 13341-13343 CC denotes or
T3312 13344-13348 NN denotes DMC1
T3313 13348-13350 , denotes ,
T3314 13350-13360 NNS denotes components
T3315 13361-13363 IN denotes of
T3316 13364-13369 JJ denotes early
T3317 13370-13383 NN denotes recombination
T3318 13384-13391 NNS denotes nodules
T3319 13392-13393 -LRB- denotes (
T3320 13393-13397 NNS denotes ERNs
T3321 13397-13398 -RRB- denotes )
T3322 13398-13400 , denotes ,
T3308 13400-13403 VBD denotes was
T3323 13404-13408 RB denotes also
T3324 13409-13416 JJ denotes present
T3325 13417-13419 IN denotes as
T3326 13420-13428 JJ denotes abundant
T3327 13429-13433 NNS denotes foci
T3328 13434-13436 IN denotes in
T3329 13437-13445 NN denotes Trip13Gt
T3331 13445-13446 HYPH denotes /
T3330 13446-13448 NN denotes Gt
T3333 13449-13457 NN denotes zygotene
T3332 13458-13471 NNS denotes spermatocytes
T3334 13472-13473 -LRB- denotes (
T3336 13473-13484 JJ denotes unpublished
T3335 13485-13489 NNS denotes data
T3337 13489-13490 : denotes ;
T3338 13491-13494 DT denotes the
T3340 13495-13505 JJ denotes anti-RAD51
T3339 13506-13514 NN denotes antibody
T3341 13515-13527 VBZ denotes cross-reacts
T3342 13528-13532 IN denotes with
T3343 13533-13537 NN denotes DMC1
T3344 13537-13538 -RRB- denotes )
T3345 13538-13540 , denotes ,
T3346 13540-13550 VBG denotes indicating
T3347 13551-13555 IN denotes that
T3349 13556-13571 JJ denotes recombinational
T3350 13572-13578 NN denotes repair
T3351 13579-13581 IN denotes of
T3352 13582-13586 NNS denotes DSBs
T3353 13587-13589 VBZ denotes is
T3348 13590-13599 VBN denotes initiated
T3354 13599-13600 . denotes .
T3355 13600-13848 sentence denotes The cohesin complex, which is essential for completion and/or maintenance of synaptic associations, appeared to assemble normally as judged by immunolabeling for the meiosis-specific cohesins STAG3 (Figure S2C and S2D) and REC8 (unpublished data).
T3356 13601-13604 DT denotes The
T3358 13605-13612 NN denotes cohesin
T3357 13613-13620 NN denotes complex
T3360 13620-13622 , denotes ,
T3361 13622-13627 WDT denotes which
T3362 13628-13630 VBZ denotes is
T3363 13631-13640 JJ denotes essential
T3364 13641-13644 IN denotes for
T3365 13645-13655 NN denotes completion
T3366 13656-13659 CC denotes and
T3367 13659-13660 HYPH denotes /
T3368 13660-13662 CC denotes or
T3369 13663-13674 NN denotes maintenance
T3370 13675-13677 IN denotes of
T3371 13678-13686 JJ denotes synaptic
T3372 13687-13699 NNS denotes associations
T3373 13699-13701 , denotes ,
T3359 13701-13709 VBD denotes appeared
T3374 13710-13712 TO denotes to
T3375 13713-13721 VB denotes assemble
T3376 13722-13730 RB denotes normally
T3377 13731-13733 IN denotes as
T3378 13734-13740 VBN denotes judged
T3379 13741-13743 IN denotes by
T3380 13744-13758 VBG denotes immunolabeling
T3381 13759-13762 IN denotes for
T3382 13763-13766 DT denotes the
T3384 13767-13774 NN denotes meiosis
T3386 13774-13775 HYPH denotes -
T3385 13775-13783 JJ denotes specific
T3387 13784-13792 NNS denotes cohesins
T3383 13793-13798 NN denotes STAG3
T3388 13799-13800 -LRB- denotes (
T3390 13800-13806 NN denotes Figure
T3389 13807-13810 NN denotes S2C
T3391 13811-13814 CC denotes and
T3392 13815-13818 NN denotes S2D
T3393 13818-13819 -RRB- denotes )
T3394 13820-13823 CC denotes and
T3395 13824-13828 NN denotes REC8
T3396 13829-13830 -LRB- denotes (
T3398 13830-13841 JJ denotes unpublished
T3397 13842-13846 NNS denotes data
T3399 13846-13847 -RRB- denotes )
T3400 13847-13848 . denotes .
T3401 13848-14078 sentence denotes Because yeast PCH2 localizes to telomeres in a Sir3p-dependent manner, we tested for possible telomere defects by immunolabeling for TRF2, a component of a protein complex that plays an essential role in telomere protection [19].
T3402 13849-13856 IN denotes Because
T3404 13857-13862 NN denotes yeast
T3405 13863-13867 NN denotes PCH2
T3403 13868-13877 VBZ denotes localizes
T3407 13878-13880 IN denotes to
T3408 13881-13890 NNS denotes telomeres
T3409 13891-13893 IN denotes in
T3410 13894-13895 DT denotes a
T3412 13896-13901 NN denotes Sir3p
T3414 13901-13902 HYPH denotes -
T3413 13902-13911 JJ denotes dependent
T3411 13912-13918 NN denotes manner
T3415 13918-13920 , denotes ,
T3416 13920-13922 PRP denotes we
T3406 13923-13929 VBD denotes tested
T3417 13930-13933 IN denotes for
T3418 13934-13942 JJ denotes possible
T3420 13943-13951 NN denotes telomere
T3419 13952-13959 NNS denotes defects
T3421 13960-13962 IN denotes by
T3422 13963-13977 VBG denotes immunolabeling
T3423 13978-13981 IN denotes for
T3424 13982-13986 NN denotes TRF2
T3425 13986-13988 , denotes ,
T3426 13988-13989 DT denotes a
T3427 13990-13999 NN denotes component
T3428 14000-14002 IN denotes of
T3429 14003-14004 DT denotes a
T3431 14005-14012 NN denotes protein
T3430 14013-14020 NN denotes complex
T3432 14021-14025 WDT denotes that
T3433 14026-14031 VBZ denotes plays
T3434 14032-14034 DT denotes an
T3436 14035-14044 JJ denotes essential
T3435 14045-14049 NN denotes role
T3437 14050-14052 IN denotes in
T3438 14053-14061 NN denotes telomere
T3439 14062-14072 NN denotes protection
T3440 14073-14074 -LRB- denotes [
T3441 14074-14076 CD denotes 19
T3442 14076-14077 -RRB- denotes ]
T3443 14077-14078 . denotes .
T3444 14078-14200 sentence denotes It was localized to telomeres of both fully synapsed and telomerically asynaptic mutant chromosomes (Figure S2E and S2F).
T3445 14079-14081 PRP denotes It
T3447 14082-14085 VBD denotes was
T3446 14086-14095 VBN denotes localized
T3448 14096-14098 IN denotes to
T3449 14099-14108 NNS denotes telomeres
T3450 14109-14111 IN denotes of
T3451 14112-14116 CC denotes both
T3453 14117-14122 RB denotes fully
T3452 14123-14131 VBN denotes synapsed
T3455 14132-14135 CC denotes and
T3456 14136-14149 RB denotes telomerically
T3457 14150-14159 JJ denotes asynaptic
T3458 14160-14166 NN denotes mutant
T3454 14167-14178 NNS denotes chromosomes
T3459 14179-14180 -LRB- denotes (
T3461 14180-14186 NN denotes Figure
T3460 14187-14190 NN denotes S2E
T3462 14191-14194 CC denotes and
T3463 14195-14198 NN denotes S2F
T3464 14198-14199 -RRB- denotes )
T3465 14199-14200 . denotes .
T3466 14200-14373 sentence denotes Defects in DSB repair became apparent in pachynema upon probing of mutant spermatocyte nuclei with antibodies against molecules involved in various stages of recombination.
T3467 14201-14208 NNS denotes Defects
T3469 14209-14211 IN denotes in
T3470 14212-14215 NN denotes DSB
T3471 14216-14222 NN denotes repair
T3468 14223-14229 VBD denotes became
T3472 14230-14238 JJ denotes apparent
T3473 14239-14241 IN denotes in
T3474 14242-14251 NN denotes pachynema
T3475 14252-14256 IN denotes upon
T3476 14257-14264 NN denotes probing
T3477 14265-14267 IN denotes of
T3478 14268-14274 NN denotes mutant
T3480 14275-14287 NN denotes spermatocyte
T3479 14288-14294 NNS denotes nuclei
T3481 14295-14299 IN denotes with
T3482 14300-14310 NNS denotes antibodies
T3483 14311-14318 IN denotes against
T3484 14319-14328 NNS denotes molecules
T3485 14329-14337 VBN denotes involved
T3486 14338-14340 IN denotes in
T3487 14341-14348 JJ denotes various
T3488 14349-14355 NNS denotes stages
T3489 14356-14358 IN denotes of
T3490 14359-14372 NN denotes recombination
T3491 14372-14373 . denotes .
T3492 14373-14586 sentence denotes In >99% of Trip13Gt/Gt chromosome spreads, BLM helicase (Figure 4C and 4D), RAD51/DMC1 (Figure 4E and 4F), γH2AX (Figure 4G and 4H), and TOPBP1 (Figure 4I and 4J) all persisted abnormally on synapsed chromosomes.
T3493 14374-14376 IN denotes In
T3495 14377-14378 SYM denotes >
T3496 14378-14380 CD denotes 99
T3497 14380-14381 NN denotes %
T3498 14382-14384 IN denotes of
T3499 14385-14393 NN denotes Trip13Gt
T3501 14393-14394 HYPH denotes /
T3500 14394-14396 NN denotes Gt
T3502 14397-14407 NN denotes chromosome
T3503 14408-14415 NNS denotes spreads
T3504 14415-14417 , denotes ,
T3505 14417-14420 NN denotes BLM
T3506 14421-14429 NN denotes helicase
T3507 14430-14431 -LRB- denotes (
T3509 14431-14437 NN denotes Figure
T3508 14438-14440 NN denotes 4C
T3510 14441-14444 CC denotes and
T3511 14445-14447 NN denotes 4D
T3512 14447-14448 -RRB- denotes )
T3513 14448-14450 , denotes ,
T3514 14450-14455 NN denotes RAD51
T3516 14455-14456 HYPH denotes /
T3515 14456-14460 NN denotes DMC1
T3517 14461-14462 -LRB- denotes (
T3519 14462-14468 NN denotes Figure
T3518 14469-14471 NN denotes 4E
T3520 14472-14475 CC denotes and
T3521 14476-14478 NN denotes 4F
T3522 14478-14479 -RRB- denotes )
T3523 14479-14481 , denotes ,
T3524 14481-14486 NN denotes γH2AX
T3525 14487-14488 -LRB- denotes (
T3527 14488-14494 NN denotes Figure
T3526 14495-14497 NN denotes 4G
T3528 14498-14501 CC denotes and
T3529 14502-14504 NN denotes 4H
T3530 14504-14505 -RRB- denotes )
T3531 14505-14507 , denotes ,
T3532 14507-14510 CC denotes and
T3533 14511-14517 NN denotes TOPBP1
T3534 14518-14519 -LRB- denotes (
T3535 14519-14525 NN denotes Figure
T3536 14526-14528 NN denotes 4I
T3537 14529-14532 CC denotes and
T3538 14533-14535 NN denotes 4J
T3539 14535-14536 -RRB- denotes )
T3540 14537-14540 DT denotes all
T3494 14541-14550 VBD denotes persisted
T3541 14551-14561 RB denotes abnormally
T3542 14562-14564 IN denotes on
T3543 14565-14573 VBN denotes synapsed
T3544 14574-14585 NNS denotes chromosomes
T3545 14585-14586 . denotes .
T3546 14586-14804 sentence denotes For RAD51/DMC1, mutant pachytene spermatocytes contained 138 ± 6 foci (compared to 11 ± 3 foci in wild type, most of which were on the XY body), down from 218 ± 13 in zygonema (compared to 220 ± 13 foci in wild type).
T3547 14587-14590 IN denotes For
T3549 14591-14596 NN denotes RAD51
T3551 14596-14597 HYPH denotes /
T3550 14597-14601 NN denotes DMC1
T3552 14601-14603 , denotes ,
T3553 14603-14609 NN denotes mutant
T3554 14610-14619 NN denotes pachytene
T3555 14620-14633 NNS denotes spermatocytes
T3548 14634-14643 VBD denotes contained
T3556 14644-14647 CD denotes 138
T3558 14648-14649 SYM denotes ±
T3557 14650-14651 CD denotes 6
T3559 14652-14656 NNS denotes foci
T3560 14657-14658 -LRB- denotes (
T3561 14658-14666 VBN denotes compared
T3562 14667-14669 IN denotes to
T3563 14670-14672 CD denotes 11
T3565 14673-14674 SYM denotes ±
T3564 14675-14676 CD denotes 3
T3566 14677-14681 NNS denotes foci
T3567 14682-14684 IN denotes in
T3568 14685-14689 JJ denotes wild
T3569 14690-14694 NN denotes type
T3570 14694-14696 , denotes ,
T3571 14696-14700 JJS denotes most
T3573 14701-14703 IN denotes of
T3574 14704-14709 WDT denotes which
T3572 14710-14714 VBD denotes were
T3575 14715-14717 IN denotes on
T3576 14718-14721 DT denotes the
T3578 14722-14724 NN denotes XY
T3577 14725-14729 NN denotes body
T3579 14729-14730 -RRB- denotes )
T3580 14730-14732 , denotes ,
T3581 14732-14736 RB denotes down
T3582 14737-14741 IN denotes from
T3583 14742-14745 CD denotes 218
T3585 14746-14747 SYM denotes ±
T3584 14748-14750 CD denotes 13
T3586 14751-14753 IN denotes in
T3587 14754-14762 NN denotes zygonema
T3588 14763-14764 -LRB- denotes (
T3589 14764-14772 VBN denotes compared
T3590 14773-14775 IN denotes to
T3591 14776-14779 CD denotes 220
T3593 14780-14781 SYM denotes ±
T3592 14782-14784 CD denotes 13
T3594 14785-14789 NNS denotes foci
T3595 14790-14792 IN denotes in
T3596 14793-14797 JJ denotes wild
T3597 14798-14802 NN denotes type
T3598 14802-14803 -RRB- denotes )
T3599 14803-14804 . denotes .
T3600 14804-14915 sentence denotes TOPBP1 is a DNA damage–checkpoint protein involved in ATM protein–dependent activation of ATR protein [20,21].
T3601 14805-14811 NN denotes TOPBP1
T3602 14812-14814 VBZ denotes is
T3603 14815-14816 DT denotes a
T3605 14817-14820 NN denotes DNA
T3606 14821-14827 NN denotes damage
T3608 14827-14828 HYPH denotes –
T3607 14828-14838 NN denotes checkpoint
T3604 14839-14846 NN denotes protein
T3609 14847-14855 VBN denotes involved
T3610 14856-14858 IN denotes in
T3611 14859-14862 NN denotes ATM
T3612 14863-14870 NN denotes protein
T3614 14870-14871 HYPH denotes –
T3613 14871-14880 JJ denotes dependent
T3615 14881-14891 NN denotes activation
T3616 14892-14894 IN denotes of
T3617 14895-14898 NN denotes ATR
T3618 14899-14906 NN denotes protein
T3619 14907-14908 -LRB- denotes [
T3621 14908-14910 CD denotes 20
T3622 14910-14911 , denotes ,
T3620 14911-14913 CD denotes 21
T3623 14913-14914 -RRB- denotes ]
T3624 14914-14915 . denotes .
T3625 14915-14993 sentence denotes It binds sites of DSBs and unsynapsed regions of meiotic chromosomes [22,23].
T3626 14916-14918 PRP denotes It
T3627 14919-14924 VBZ denotes binds
T3628 14925-14930 NNS denotes sites
T3629 14931-14933 IN denotes of
T3630 14934-14938 NNS denotes DSBs
T3631 14939-14942 CC denotes and
T3632 14943-14953 JJ denotes unsynapsed
T3633 14954-14961 NNS denotes regions
T3634 14962-14964 IN denotes of
T3635 14965-14972 JJ denotes meiotic
T3636 14973-14984 NNS denotes chromosomes
T3637 14985-14986 -LRB- denotes [
T3639 14986-14988 CD denotes 22
T3640 14988-14989 , denotes ,
T3638 14989-14991 CD denotes 23
T3641 14991-14992 -RRB- denotes ]
T3642 14992-14993 . denotes .
T3643 14993-15149 sentence denotes BLM has been reported to colocalize with markers (RPA and MSH4) of recombination at sites distinct from those that become resolved as crossovers (CO) [24].
T3644 14994-14997 NN denotes BLM
T3646 14998-15001 VBZ denotes has
T3647 15002-15006 VBN denotes been
T3645 15007-15015 VBN denotes reported
T3648 15016-15018 TO denotes to
T3649 15019-15029 VB denotes colocalize
T3650 15030-15034 IN denotes with
T3651 15035-15042 NNS denotes markers
T3653 15043-15044 -LRB- denotes (
T3652 15044-15047 NN denotes RPA
T3654 15048-15051 CC denotes and
T3655 15052-15056 NN denotes MSH4
T3656 15056-15057 -RRB- denotes )
T3657 15058-15060 IN denotes of
T3658 15061-15074 NN denotes recombination
T3659 15075-15077 IN denotes at
T3660 15078-15083 NNS denotes sites
T3661 15084-15092 JJ denotes distinct
T3662 15093-15097 IN denotes from
T3663 15098-15103 DT denotes those
T3664 15104-15108 WDT denotes that
T3665 15109-15115 VBP denotes become
T3666 15116-15124 JJ denotes resolved
T3667 15125-15127 IN denotes as
T3668 15128-15138 NNS denotes crossovers
T3669 15139-15140 -LRB- denotes (
T3670 15140-15142 NN denotes CO
T3671 15142-15143 -RRB- denotes )
T3672 15144-15145 -LRB- denotes [
T3673 15145-15147 CD denotes 24
T3674 15147-15148 -RRB- denotes ]
T3675 15148-15149 . denotes .
T3676 15149-15340 sentence denotes We therefore assessed the distribution of RPA, the ssDNA binding protein, which is normally present at focal sites of synapsing meiotic chromosomes before disappearing in mid-pachynema [25].
T3677 15150-15152 PRP denotes We
T3679 15153-15162 RB denotes therefore
T3678 15163-15171 VBD denotes assessed
T3680 15172-15175 DT denotes the
T3681 15176-15188 NN denotes distribution
T3682 15189-15191 IN denotes of
T3683 15192-15195 NN denotes RPA
T3684 15195-15197 , denotes ,
T3685 15197-15200 DT denotes the
T3687 15201-15206 NN denotes ssDNA
T3688 15207-15214 NN denotes binding
T3686 15215-15222 NN denotes protein
T3689 15222-15224 , denotes ,
T3690 15224-15229 WDT denotes which
T3691 15230-15232 VBZ denotes is
T3692 15233-15241 RB denotes normally
T3693 15242-15249 JJ denotes present
T3694 15250-15252 IN denotes at
T3695 15253-15258 JJ denotes focal
T3696 15259-15264 NNS denotes sites
T3697 15265-15267 IN denotes of
T3698 15268-15277 VBG denotes synapsing
T3700 15278-15285 JJ denotes meiotic
T3699 15286-15297 NNS denotes chromosomes
T3701 15298-15304 IN denotes before
T3702 15305-15317 VBG denotes disappearing
T3703 15318-15320 IN denotes in
T3704 15321-15334 NN denotes mid-pachynema
T3705 15335-15336 -LRB- denotes [
T3706 15336-15338 CD denotes 25
T3707 15338-15339 -RRB- denotes ]
T3708 15339-15340 . denotes .
T3709 15340-15407 sentence denotes It is thought to bind D-loops of recombination intermediates [26].
T3710 15341-15343 PRP denotes It
T3712 15344-15346 VBZ denotes is
T3711 15347-15354 VBN denotes thought
T3713 15355-15357 TO denotes to
T3714 15358-15362 VB denotes bind
T3715 15363-15364 NN denotes D
T3717 15364-15365 HYPH denotes -
T3716 15365-15370 NNS denotes loops
T3718 15371-15373 IN denotes of
T3719 15374-15387 NN denotes recombination
T3720 15388-15401 NNS denotes intermediates
T3721 15402-15403 -LRB- denotes [
T3722 15403-15405 CD denotes 26
T3723 15405-15406 -RRB- denotes ]
T3724 15406-15407 . denotes .
T3725 15407-15478 sentence denotes RPA also persisted on pachytene mutant chromosomes (Figure 4K and 4L).
T3726 15408-15411 NN denotes RPA
T3728 15412-15416 RB denotes also
T3727 15417-15426 VBD denotes persisted
T3729 15427-15429 IN denotes on
T3730 15430-15439 NN denotes pachytene
T3732 15440-15446 NN denotes mutant
T3731 15447-15458 NNS denotes chromosomes
T3733 15459-15460 -LRB- denotes (
T3735 15460-15466 NN denotes Figure
T3734 15467-15469 NN denotes 4K
T3736 15470-15473 CC denotes and
T3737 15474-15476 NN denotes 4L
T3738 15476-15477 -RRB- denotes )
T3739 15477-15478 . denotes .
T3740 15478-15628 sentence denotes These data indicate that unrepaired DSBs, or unresolved recombination intermediates, remain in pachynema and activate a DNA damage checkpoint system.
T3741 15479-15484 DT denotes These
T3742 15485-15489 NNS denotes data
T3743 15490-15498 VBP denotes indicate
T3744 15499-15503 IN denotes that
T3746 15504-15514 JJ denotes unrepaired
T3747 15515-15519 NNS denotes DSBs
T3748 15519-15521 , denotes ,
T3749 15521-15523 CC denotes or
T3750 15524-15534 JJ denotes unresolved
T3752 15535-15548 NN denotes recombination
T3751 15549-15562 NNS denotes intermediates
T3753 15562-15564 , denotes ,
T3745 15564-15570 VBP denotes remain
T3754 15571-15573 IN denotes in
T3755 15574-15583 NN denotes pachynema
T3756 15584-15587 CC denotes and
T3757 15588-15596 VBP denotes activate
T3758 15597-15598 DT denotes a
T3760 15599-15602 NN denotes DNA
T3761 15603-15609 NN denotes damage
T3762 15610-15620 NN denotes checkpoint
T3759 15621-15627 NN denotes system
T3763 15627-15628 . denotes .
T3764 15628-15866 sentence denotes It should be noted that chromosomes affected by meiotic sex chromosome inactivation (MSCI) and meiotic silencing of unpaired chromatin (MSUC) are heavily stained by antibodies for several DSB repair-associated molecules, including γH2AX.
T3765 15629-15631 PRP denotes It
T3767 15632-15638 MD denotes should
T3768 15639-15641 VB denotes be
T3766 15642-15647 VBN denotes noted
T3769 15648-15652 IN denotes that
T3771 15653-15664 NNS denotes chromosomes
T3772 15665-15673 VBN denotes affected
T3773 15674-15676 IN denotes by
T3774 15677-15684 JJ denotes meiotic
T3776 15685-15688 NN denotes sex
T3777 15689-15699 NN denotes chromosome
T3775 15700-15712 NN denotes inactivation
T3778 15713-15714 -LRB- denotes (
T3779 15714-15718 NN denotes MSCI
T3780 15718-15719 -RRB- denotes )
T3781 15720-15723 CC denotes and
T3782 15724-15731 JJ denotes meiotic
T3783 15732-15741 NN denotes silencing
T3784 15742-15744 IN denotes of
T3785 15745-15753 JJ denotes unpaired
T3786 15754-15763 NN denotes chromatin
T3787 15764-15765 -LRB- denotes (
T3788 15765-15769 NN denotes MSUC
T3789 15769-15770 -RRB- denotes )
T3790 15771-15774 VBP denotes are
T3791 15775-15782 RB denotes heavily
T3770 15783-15790 VBN denotes stained
T3792 15791-15793 IN denotes by
T3793 15794-15804 NNS denotes antibodies
T3794 15805-15808 IN denotes for
T3795 15809-15816 JJ denotes several
T3797 15817-15820 NN denotes DSB
T3798 15821-15827 NN denotes repair
T3800 15827-15828 HYPH denotes -
T3799 15828-15838 VBN denotes associated
T3796 15839-15848 NNS denotes molecules
T3801 15848-15850 , denotes ,
T3802 15850-15859 VBG denotes including
T3803 15860-15865 NN denotes γH2AX
T3804 15865-15866 . denotes .
T3805 15866-15946 sentence denotes H2AX phosphorylation due to MSCI and MSUC is conducted by ATR, not ATM [27–29].
T3806 15867-15871 NN denotes H2AX
T3807 15872-15887 NN denotes phosphorylation
T3809 15888-15891 IN denotes due
T3810 15892-15894 IN denotes to
T3811 15895-15899 NN denotes MSCI
T3812 15900-15903 CC denotes and
T3813 15904-15908 NN denotes MSUC
T3814 15909-15911 VBZ denotes is
T3808 15912-15921 VBN denotes conducted
T3815 15922-15924 IN denotes by
T3816 15925-15928 NN denotes ATR
T3817 15928-15930 , denotes ,
T3818 15930-15933 CC denotes not
T3819 15934-15937 NN denotes ATM
T3820 15938-15939 -LRB- denotes [
T3821 15939-15941 CD denotes 27
T3822 15941-15942 SYM denotes –
T3823 15942-15944 CD denotes 29
T3824 15944-15945 -RRB- denotes ]
T3825 15945-15946 . denotes .
T3826 15946-16204 sentence denotes Since mutant chromosomes are fully synapsed, and MSUC is known to occur only as a result of asynapsis, the decoration of Trip13Gt/Gt chromosomes with DNA repair markers is probably attributable to incomplete DNA repair rather than transcriptional silencing.
T3827 15947-15952 IN denotes Since
T3829 15953-15959 NN denotes mutant
T3830 15960-15971 NNS denotes chromosomes
T3831 15972-15975 VBP denotes are
T3832 15976-15981 RB denotes fully
T3828 15982-15990 VBN denotes synapsed
T3834 15990-15992 , denotes ,
T3835 15992-15995 CC denotes and
T3836 15996-16000 NN denotes MSUC
T3838 16001-16003 VBZ denotes is
T3837 16004-16009 VBN denotes known
T3839 16010-16012 TO denotes to
T3840 16013-16018 VB denotes occur
T3841 16019-16023 RB denotes only
T3842 16024-16026 IN denotes as
T3843 16027-16028 DT denotes a
T3844 16029-16035 NN denotes result
T3845 16036-16038 IN denotes of
T3846 16039-16048 NN denotes asynapsis
T3847 16048-16050 , denotes ,
T3848 16050-16053 DT denotes the
T3849 16054-16064 NN denotes decoration
T3850 16065-16067 IN denotes of
T3851 16068-16076 NN denotes Trip13Gt
T3853 16076-16077 HYPH denotes /
T3852 16077-16079 NN denotes Gt
T3854 16080-16091 NNS denotes chromosomes
T3855 16092-16096 IN denotes with
T3856 16097-16100 NN denotes DNA
T3857 16101-16107 NN denotes repair
T3858 16108-16115 NNS denotes markers
T3833 16116-16118 VBZ denotes is
T3859 16119-16127 RB denotes probably
T3860 16128-16140 JJ denotes attributable
T3861 16141-16143 IN denotes to
T3862 16144-16154 JJ denotes incomplete
T3864 16155-16158 NN denotes DNA
T3863 16159-16165 NN denotes repair
T3865 16166-16172 RB denotes rather
T3866 16173-16177 IN denotes than
T3867 16178-16193 JJ denotes transcriptional
T3868 16194-16203 NN denotes silencing
T3869 16203-16204 . denotes .
T3870 16204-16565 sentence denotes Consistent with the presence of rare (<1%) Trip13Gt/Gt pachytene spermatocytes devoid of persistent DNA repair markers, and testis histology showing some degree of postmeiotic progression (Figure 3G), we observed both diplotene nuclei that lacked autosomal RAD51/DMC1 and γH2AX (Figure S3A–S3D), and also metaphase I spreads with 20 bivalents (Figure S3E–S3F).
T3871 16205-16215 JJ denotes Consistent
T3873 16216-16220 IN denotes with
T3874 16221-16224 DT denotes the
T3875 16225-16233 NN denotes presence
T3876 16234-16236 IN denotes of
T3877 16237-16241 JJ denotes rare
T3879 16242-16243 -LRB- denotes (
T3881 16243-16244 SYM denotes <
T3882 16244-16245 CD denotes 1
T3880 16245-16246 NN denotes %
T3883 16246-16247 -RRB- denotes )
T3884 16248-16256 NN denotes Trip13Gt
T3886 16256-16257 HYPH denotes /
T3885 16257-16259 NN denotes Gt
T3887 16260-16269 NN denotes pachytene
T3878 16270-16283 NNS denotes spermatocytes
T3888 16284-16290 JJ denotes devoid
T3889 16291-16293 IN denotes of
T3890 16294-16304 JJ denotes persistent
T3892 16305-16308 NN denotes DNA
T3893 16309-16315 NN denotes repair
T3891 16316-16323 NNS denotes markers
T3894 16323-16325 , denotes ,
T3895 16325-16328 CC denotes and
T3896 16329-16335 NN denotes testis
T3897 16336-16345 NN denotes histology
T3898 16346-16353 VBG denotes showing
T3899 16354-16358 DT denotes some
T3900 16359-16365 NN denotes degree
T3901 16366-16368 IN denotes of
T3902 16369-16380 JJ denotes postmeiotic
T3903 16381-16392 NN denotes progression
T3904 16393-16394 -LRB- denotes (
T3906 16394-16400 NN denotes Figure
T3905 16401-16403 NN denotes 3G
T3907 16403-16404 -RRB- denotes )
T3908 16404-16406 , denotes ,
T3909 16406-16408 PRP denotes we
T3872 16409-16417 VBD denotes observed
T3910 16418-16422 CC denotes both
T3912 16423-16432 NN denotes diplotene
T3911 16433-16439 NNS denotes nuclei
T3913 16440-16444 WDT denotes that
T3914 16445-16451 VBD denotes lacked
T3915 16452-16461 JJ denotes autosomal
T3917 16462-16467 NN denotes RAD51
T3918 16467-16468 HYPH denotes /
T3916 16468-16472 NN denotes DMC1
T3919 16473-16476 CC denotes and
T3920 16477-16482 NN denotes γH2AX
T3921 16483-16484 -LRB- denotes (
T3923 16484-16490 NN denotes Figure
T3924 16491-16494 NN denotes S3A
T3925 16494-16495 SYM denotes –
T3922 16495-16498 NN denotes S3D
T3926 16498-16499 -RRB- denotes )
T3927 16499-16501 , denotes ,
T3928 16501-16504 CC denotes and
T3929 16505-16509 RB denotes also
T3930 16510-16519 NN denotes metaphase
T3932 16520-16521 CD denotes I
T3931 16522-16529 NNS denotes spreads
T3933 16530-16534 IN denotes with
T3934 16535-16537 CD denotes 20
T3935 16538-16547 NNS denotes bivalents
T3936 16548-16549 -LRB- denotes (
T3938 16549-16555 NN denotes Figure
T3939 16556-16559 NN denotes S3E
T3940 16559-16560 SYM denotes –
T3937 16560-16563 NN denotes S3F
T3941 16563-16564 -RRB- denotes )
T3942 16564-16565 . denotes .
T3943 16565-16715 sentence denotes Since Trip13Gt may not be a complete null, these diplotene and metaphase I spermatocytes might arise by virtue of having sufficient wild-type TRIP13.
T3944 16566-16571 IN denotes Since
T3946 16572-16580 NN denotes Trip13Gt
T3947 16581-16584 MD denotes may
T3948 16585-16588 RB denotes not
T3945 16589-16591 VB denotes be
T3950 16592-16593 DT denotes a
T3952 16594-16602 JJ denotes complete
T3951 16603-16607 NN denotes null
T3953 16607-16609 , denotes ,
T3954 16609-16614 DT denotes these
T3956 16615-16624 NN denotes diplotene
T3957 16625-16628 CC denotes and
T3958 16629-16638 NN denotes metaphase
T3959 16639-16640 CD denotes I
T3955 16641-16654 NNS denotes spermatocytes
T3960 16655-16660 MD denotes might
T3949 16661-16666 VB denotes arise
T3961 16667-16669 IN denotes by
T3962 16670-16676 NN denotes virtue
T3963 16677-16679 IN denotes of
T3964 16680-16686 VBG denotes having
T3965 16687-16697 JJ denotes sufficient
T3967 16698-16702 JJ denotes wild
T3969 16702-16703 HYPH denotes -
T3968 16703-16707 NN denotes type
T3966 16708-16714 NN denotes TRIP13
T3970 16714-16715 . denotes .
T4133 16717-16719 NN denotes CO
T4135 16719-16720 HYPH denotes -
T4134 16720-16730 VBN denotes Associated
T4136 16731-16738 NNS denotes Markers
T4137 16739-16745 VBP denotes Appear
T4138 16746-16754 RB denotes Normally
T4139 16755-16757 IN denotes in
T4140 16758-16761 DT denotes the
T4141 16762-16769 NN denotes Absence
T4142 16770-16772 IN denotes of
T4143 16773-16779 NN denotes TRIP13
T4144 16779-16958 sentence denotes The persistence of BLM on Trip13Gt/Gt spermatocyte chromosomes suggests that at least a subset of the unrepaired DSBs correspond to sites of defective NCO recombinational repair.
T4145 16780-16783 DT denotes The
T4146 16784-16795 NN denotes persistence
T4148 16796-16798 IN denotes of
T4149 16799-16802 NN denotes BLM
T4150 16803-16805 IN denotes on
T4151 16806-16814 NN denotes Trip13Gt
T4153 16814-16815 HYPH denotes /
T4152 16815-16817 NN denotes Gt
T4154 16818-16830 NN denotes spermatocyte
T4155 16831-16842 NNS denotes chromosomes
T4147 16843-16851 VBZ denotes suggests
T4156 16852-16856 IN denotes that
T4158 16857-16859 RB denotes at
T4159 16860-16865 RBS denotes least
T4161 16866-16867 DT denotes a
T4160 16868-16874 NN denotes subset
T4162 16875-16877 IN denotes of
T4163 16878-16881 DT denotes the
T4165 16882-16892 JJ denotes unrepaired
T4164 16893-16897 NNS denotes DSBs
T4157 16898-16908 VBP denotes correspond
T4166 16909-16911 IN denotes to
T4167 16912-16917 NNS denotes sites
T4168 16918-16920 IN denotes of
T4169 16921-16930 JJ denotes defective
T4171 16931-16934 NN denotes NCO
T4172 16935-16950 JJ denotes recombinational
T4170 16951-16957 NN denotes repair
T4173 16957-16958 . denotes .
T4174 16958-17196 sentence denotes To assess whether CO recombination occurs in the mutant, we examined the distribution of the mismatch repair proteins MLH1 and MLH3, which are normally detectable as foci in mid-late pachynema and mark the locations of chiasmata [30,31].
T4175 16959-16961 TO denotes To
T4176 16962-16968 VB denotes assess
T4178 16969-16976 IN denotes whether
T4180 16977-16979 NN denotes CO
T4181 16980-16993 NN denotes recombination
T4179 16994-17000 VBZ denotes occurs
T4182 17001-17003 IN denotes in
T4183 17004-17007 DT denotes the
T4184 17008-17014 NN denotes mutant
T4185 17014-17016 , denotes ,
T4186 17016-17018 PRP denotes we
T4177 17019-17027 VBD denotes examined
T4187 17028-17031 DT denotes the
T4188 17032-17044 NN denotes distribution
T4189 17045-17047 IN denotes of
T4190 17048-17051 DT denotes the
T4192 17052-17060 NN denotes mismatch
T4193 17061-17067 NN denotes repair
T4191 17068-17076 NN denotes proteins
T4194 17077-17081 NN denotes MLH1
T4195 17082-17085 CC denotes and
T4196 17086-17090 NN denotes MLH3
T4197 17090-17092 , denotes ,
T4198 17092-17097 WDT denotes which
T4199 17098-17101 VBP denotes are
T4200 17102-17110 RB denotes normally
T4201 17111-17121 JJ denotes detectable
T4202 17122-17124 IN denotes as
T4203 17125-17129 NNS denotes foci
T4204 17130-17132 IN denotes in
T4205 17133-17136 JJ denotes mid
T4207 17136-17137 SYM denotes -
T4206 17137-17141 JJ denotes late
T4208 17142-17151 NN denotes pachynema
T4209 17152-17155 CC denotes and
T4210 17156-17160 VBP denotes mark
T4211 17161-17164 DT denotes the
T4212 17165-17174 NNS denotes locations
T4213 17175-17177 IN denotes of
T4214 17178-17187 NNS denotes chiasmata
T4215 17188-17189 -LRB- denotes [
T4217 17189-17191 CD denotes 30
T4218 17191-17192 , denotes ,
T4216 17192-17194 CD denotes 31
T4219 17194-17195 -RRB- denotes ]
T4220 17195-17196 . denotes .
T4221 17196-17414 sentence denotes Remarkably, MLH1/3 foci were formed; we observed 1–2 foci/chromosome as in wild type and at typical overall levels (MLH3 = 23 ± 2, N = 10; [30,32]) on mid-late pachytene chromosomes (Figure 4M and 4N; MLH1 not shown).
T4222 17197-17207 RB denotes Remarkably
T4224 17207-17209 , denotes ,
T4225 17209-17213 NN denotes MLH1
T4227 17213-17214 HYPH denotes /
T4228 17214-17215 CD denotes 3
T4226 17216-17220 NNS denotes foci
T4229 17221-17225 VBD denotes were
T4223 17226-17232 VBN denotes formed
T4231 17232-17233 : denotes ;
T4232 17234-17236 PRP denotes we
T4230 17237-17245 VBD denotes observed
T4233 17246-17247 CD denotes 1
T4235 17247-17248 SYM denotes –
T4234 17248-17249 CD denotes 2
T4236 17250-17254 NNS denotes foci
T4237 17254-17255 SYM denotes /
T4238 17255-17265 NN denotes chromosome
T4239 17266-17268 IN denotes as
T4240 17269-17271 IN denotes in
T4241 17272-17276 JJ denotes wild
T4242 17277-17281 NN denotes type
T4243 17282-17285 CC denotes and
T4244 17286-17288 IN denotes at
T4245 17289-17296 JJ denotes typical
T4247 17297-17304 JJ denotes overall
T4246 17305-17311 NNS denotes levels
T4248 17312-17313 -LRB- denotes (
T4250 17313-17317 NN denotes MLH3
T4252 17318-17319 SYM denotes =
T4253 17320-17322 CD denotes 23
T4254 17323-17324 SYM denotes ±
T4251 17325-17326 CD denotes 2
T4255 17326-17328 , denotes ,
T4256 17328-17329 NN denotes N
T4257 17330-17331 SYM denotes =
T4249 17332-17334 CD denotes 10
T4258 17334-17335 : denotes ;
T4259 17336-17337 -LRB- denotes [
T4261 17337-17339 CD denotes 30
T4262 17339-17340 , denotes ,
T4260 17340-17342 CD denotes 32
T4263 17342-17343 -RRB- denotes ]
T4264 17343-17344 -RRB- denotes )
T4265 17345-17347 IN denotes on
T4266 17348-17351 JJ denotes mid
T4268 17351-17352 SYM denotes -
T4267 17352-17356 JJ denotes late
T4269 17357-17366 NN denotes pachytene
T4270 17367-17378 NNS denotes chromosomes
T4271 17379-17380 -LRB- denotes (
T4273 17380-17386 NN denotes Figure
T4272 17387-17389 NN denotes 4M
T4274 17390-17393 CC denotes and
T4275 17394-17396 NN denotes 4N
T4276 17396-17397 : denotes ;
T4277 17398-17402 NN denotes MLH1
T4279 17403-17406 RB denotes not
T4278 17407-17412 VBN denotes shown
T4280 17412-17413 -RRB- denotes )
T4281 17413-17414 . denotes .
T4282 17414-17682 sentence denotes Since <1% of Trip13Gt/Gt pachytene nuclei had normal repair (as judged by absence of persistent DSB repair markers; see above), but most of the pachytene nuclei had MLH1/3 foci, it was unlikely that the MLH1/3 foci formed only on chromosomes with fully repaired DSBs.
T4283 17415-17420 IN denotes Since
T4285 17421-17422 SYM denotes <
T4286 17422-17423 CD denotes 1
T4287 17423-17424 NN denotes %
T4288 17425-17427 IN denotes of
T4289 17428-17436 NN denotes Trip13Gt
T4291 17436-17437 HYPH denotes /
T4290 17437-17439 NN denotes Gt
T4292 17440-17449 NN denotes pachytene
T4293 17450-17456 NNS denotes nuclei
T4284 17457-17460 VBD denotes had
T4295 17461-17467 JJ denotes normal
T4296 17468-17474 NN denotes repair
T4297 17475-17476 -LRB- denotes (
T4299 17476-17478 IN denotes as
T4300 17479-17485 VBN denotes judged
T4301 17486-17488 IN denotes by
T4302 17489-17496 NN denotes absence
T4303 17497-17499 IN denotes of
T4304 17500-17510 JJ denotes persistent
T4306 17511-17514 NN denotes DSB
T4307 17515-17521 NN denotes repair
T4305 17522-17529 NNS denotes markers
T4308 17529-17530 : denotes ;
T4298 17531-17534 VB denotes see
T4309 17535-17540 RB denotes above
T4310 17540-17541 -RRB- denotes )
T4311 17541-17543 , denotes ,
T4312 17543-17546 CC denotes but
T4313 17547-17551 JJS denotes most
T4315 17552-17554 IN denotes of
T4316 17555-17558 DT denotes the
T4318 17559-17568 NN denotes pachytene
T4317 17569-17575 NNS denotes nuclei
T4314 17576-17579 VBD denotes had
T4319 17580-17584 NN denotes MLH1
T4321 17584-17585 HYPH denotes /
T4322 17585-17586 CD denotes 3
T4320 17587-17591 NNS denotes foci
T4323 17591-17593 , denotes ,
T4324 17593-17595 PRP denotes it
T4294 17596-17599 VBD denotes was
T4325 17600-17608 JJ denotes unlikely
T4326 17609-17613 IN denotes that
T4328 17614-17617 DT denotes the
T4330 17618-17622 NN denotes MLH1
T4331 17622-17623 HYPH denotes /
T4332 17623-17624 CD denotes 3
T4329 17625-17629 NNS denotes foci
T4327 17630-17636 VBD denotes formed
T4333 17637-17641 RB denotes only
T4334 17642-17644 IN denotes on
T4335 17645-17656 NNS denotes chromosomes
T4336 17657-17661 IN denotes with
T4337 17662-17667 RB denotes fully
T4338 17668-17676 VBN denotes repaired
T4339 17677-17681 NNS denotes DSBs
T4340 17681-17682 . denotes .
T4341 17682-17759 sentence denotes To test this directly, we conducted double staining for MLH1 and RAD51/DMC1.
T4342 17683-17685 TO denotes To
T4343 17686-17690 VB denotes test
T4345 17691-17695 DT denotes this
T4346 17696-17704 RB denotes directly
T4347 17704-17706 , denotes ,
T4348 17706-17708 PRP denotes we
T4344 17709-17718 VBD denotes conducted
T4349 17719-17725 JJ denotes double
T4350 17726-17734 NN denotes staining
T4351 17735-17738 IN denotes for
T4352 17739-17743 NN denotes MLH1
T4353 17744-17747 CC denotes and
T4354 17748-17753 NN denotes RAD51
T4356 17753-17754 HYPH denotes /
T4355 17754-17758 NN denotes DMC1
T4357 17758-17759 . denotes .
T4358 17759-17862 sentence denotes MLH1 foci were present on chromosomes that also contained numerous RAD51/DMC1 foci (Figure 4O and 4P).
T4359 17760-17764 NN denotes MLH1
T4360 17765-17769 NNS denotes foci
T4361 17770-17774 VBD denotes were
T4362 17775-17782 JJ denotes present
T4363 17783-17785 IN denotes on
T4364 17786-17797 NNS denotes chromosomes
T4365 17798-17802 WDT denotes that
T4367 17803-17807 RB denotes also
T4366 17808-17817 VBD denotes contained
T4368 17818-17826 JJ denotes numerous
T4370 17827-17832 NN denotes RAD51
T4372 17832-17833 HYPH denotes /
T4371 17833-17837 NN denotes DMC1
T4369 17838-17842 NNS denotes foci
T4373 17843-17844 -LRB- denotes (
T4375 17844-17850 NN denotes Figure
T4374 17851-17853 NN denotes 4O
T4376 17854-17857 CC denotes and
T4377 17858-17860 NN denotes 4P
T4378 17860-17861 -RRB- denotes )
T4379 17861-17862 . denotes .
T4380 17862-18331 sentence denotes To assess whether these MLH1/3 foci in Trip13Gt/Gt pachytene spermatocytes represent CO events completed to a point where they could maintain interhomolog attachments though the end of prophase I, we treated testicular cells from 17.5–20.5-d-old control (+/+), Trip13Gt/Gt, and Dmc1 −/− mice with the protein phosphatase inhibitor okadaic acid (OA), a chemical that induces degradation of the SC, chromosome condensation, and premature progression to metaphase I [33].
T4381 17863-17865 TO denotes To
T4382 17866-17872 VB denotes assess
T4384 17873-17880 IN denotes whether
T4386 17881-17886 DT denotes these
T4388 17887-17891 NN denotes MLH1
T4389 17891-17892 HYPH denotes /
T4390 17892-17893 CD denotes 3
T4387 17894-17898 NNS denotes foci
T4391 17899-17901 IN denotes in
T4392 17902-17910 NN denotes Trip13Gt
T4394 17910-17911 HYPH denotes /
T4393 17911-17913 NN denotes Gt
T4395 17914-17923 NN denotes pachytene
T4396 17924-17937 NNS denotes spermatocytes
T4385 17938-17947 VBP denotes represent
T4397 17948-17950 NN denotes CO
T4398 17951-17957 NNS denotes events
T4399 17958-17967 VBN denotes completed
T4400 17968-17970 IN denotes to
T4401 17971-17972 DT denotes a
T4402 17973-17978 NN denotes point
T4403 17979-17984 WRB denotes where
T4405 17985-17989 PRP denotes they
T4406 17990-17995 MD denotes could
T4404 17996-18004 VB denotes maintain
T4407 18005-18017 JJ denotes interhomolog
T4408 18018-18029 NNS denotes attachments
T4409 18030-18036 IN denotes though
T4410 18037-18040 DT denotes the
T4411 18041-18044 NN denotes end
T4412 18045-18047 IN denotes of
T4413 18048-18056 NN denotes prophase
T4414 18057-18058 CD denotes I
T4415 18058-18060 , denotes ,
T4416 18060-18062 PRP denotes we
T4383 18063-18070 VBD denotes treated
T4417 18071-18081 JJ denotes testicular
T4418 18082-18087 NNS denotes cells
T4419 18088-18092 IN denotes from
T4420 18093-18097 CD denotes 17.5
T4422 18097-18098 SYM denotes –
T4421 18098-18102 CD denotes 20.5
T4424 18102-18103 HYPH denotes -
T4423 18103-18104 NN denotes d
T4426 18104-18105 HYPH denotes -
T4425 18105-18108 JJ denotes old
T4428 18109-18116 NN denotes control
T4429 18117-18118 -LRB- denotes (
T4430 18118-18119 SYM denotes +
T4432 18119-18120 HYPH denotes /
T4431 18120-18121 SYM denotes +
T4433 18121-18122 -RRB- denotes )
T4434 18122-18124 , denotes ,
T4435 18124-18132 NN denotes Trip13Gt
T4437 18132-18133 HYPH denotes /
T4436 18133-18135 NN denotes Gt
T4438 18135-18137 , denotes ,
T4439 18137-18140 CC denotes and
T4440 18141-18145 NN denotes Dmc1
T4441 18146-18147 SYM denotes −
T4442 18147-18148 HYPH denotes /
T4443 18148-18149 SYM denotes −
T4427 18150-18154 NNS denotes mice
T4444 18155-18159 IN denotes with
T4445 18160-18163 DT denotes the
T4447 18164-18171 NN denotes protein
T4448 18172-18183 NN denotes phosphatase
T4446 18184-18193 NN denotes inhibitor
T4449 18194-18201 JJ denotes okadaic
T4450 18202-18206 NN denotes acid
T4451 18207-18208 -LRB- denotes (
T4452 18208-18210 NN denotes OA
T4453 18210-18211 -RRB- denotes )
T4454 18211-18213 , denotes ,
T4455 18213-18214 DT denotes a
T4456 18215-18223 NN denotes chemical
T4457 18224-18228 WDT denotes that
T4458 18229-18236 VBZ denotes induces
T4459 18237-18248 NN denotes degradation
T4460 18249-18251 IN denotes of
T4461 18252-18255 DT denotes the
T4462 18256-18258 NN denotes SC
T4463 18258-18260 , denotes ,
T4464 18260-18270 NN denotes chromosome
T4465 18271-18283 NN denotes condensation
T4466 18283-18285 , denotes ,
T4467 18285-18288 CC denotes and
T4468 18289-18298 JJ denotes premature
T4469 18299-18310 NN denotes progression
T4470 18311-18313 IN denotes to
T4471 18314-18323 NN denotes metaphase
T4472 18324-18325 CD denotes I
T4473 18326-18327 -LRB- denotes [
T4474 18327-18329 CD denotes 33
T4475 18329-18330 -RRB- denotes ]
T4476 18330-18331 . denotes .
T4477 18331-18392 sentence denotes Fifteen metaphase spreads were identified for each genotype.
T4478 18332-18339 CD denotes Fifteen
T4480 18340-18349 NN denotes metaphase
T4479 18350-18357 NNS denotes spreads
T4482 18358-18362 VBD denotes were
T4481 18363-18373 VBN denotes identified
T4483 18374-18377 IN denotes for
T4484 18378-18382 DT denotes each
T4485 18383-18391 NN denotes genotype
T4486 18391-18392 . denotes .
T4487 18392-18642 sentence denotes Whereas all of the Dmc1 −/− spreads had ∼35 or more condensed chromosomes, all of the +/+ and Trip13Gt/Gt spreads had 20–25, suggesting that the MLH1/3 foci in Trip13Gt/Gt pachytene spermatocytes represent sites of completed, or near-completed, COs.
T4488 18393-18400 IN denotes Whereas
T4490 18401-18404 DT denotes all
T4491 18405-18407 IN denotes of
T4492 18408-18411 DT denotes the
T4494 18412-18416 NN denotes Dmc1
T4495 18417-18418 SYM denotes −
T4496 18418-18419 HYPH denotes /
T4497 18419-18420 SYM denotes −
T4493 18421-18428 NNS denotes spreads
T4489 18429-18432 VBD denotes had
T4499 18433-18434 SYM denotes ∼
T4500 18434-18436 CD denotes 35
T4502 18437-18439 CC denotes or
T4503 18440-18444 JJR denotes more
T4504 18445-18454 VBN denotes condensed
T4501 18455-18466 NNS denotes chromosomes
T4505 18466-18468 , denotes ,
T4506 18468-18471 DT denotes all
T4507 18472-18474 IN denotes of
T4508 18475-18478 DT denotes the
T4510 18479-18480 SYM denotes +
T4512 18480-18481 HYPH denotes /
T4511 18481-18482 SYM denotes +
T4513 18483-18486 CC denotes and
T4514 18487-18495 NN denotes Trip13Gt
T4516 18495-18496 HYPH denotes /
T4515 18496-18498 NN denotes Gt
T4509 18499-18506 NNS denotes spreads
T4498 18507-18510 VBD denotes had
T4517 18511-18513 CD denotes 20
T4519 18513-18514 SYM denotes –
T4518 18514-18516 CD denotes 25
T4520 18516-18518 , denotes ,
T4521 18518-18528 VBG denotes suggesting
T4522 18529-18533 IN denotes that
T4524 18534-18537 DT denotes the
T4526 18538-18542 NN denotes MLH1
T4527 18542-18543 HYPH denotes /
T4528 18543-18544 CD denotes 3
T4525 18545-18549 NNS denotes foci
T4529 18550-18552 IN denotes in
T4530 18553-18561 NN denotes Trip13Gt
T4532 18561-18562 HYPH denotes /
T4531 18562-18564 NN denotes Gt
T4533 18565-18574 NN denotes pachytene
T4534 18575-18588 NNS denotes spermatocytes
T4523 18589-18598 VBP denotes represent
T4535 18599-18604 NNS denotes sites
T4536 18605-18607 IN denotes of
T4537 18608-18617 JJ denotes completed
T4539 18617-18619 , denotes ,
T4540 18619-18621 CC denotes or
T4541 18622-18626 RB denotes near
T4543 18626-18627 HYPH denotes -
T4542 18627-18636 JJ denotes completed
T4544 18636-18638 , denotes ,
T4538 18638-18641 NNS denotes COs
T4545 18641-18642 . denotes .
T4546 18642-18815 sentence denotes Because the preparations were made from whole testes, it is possible that the univalent-containing metaphases from Dmc1 −/− mice were from spermatogonia, not spermatocytes.
T4547 18643-18650 IN denotes Because
T4549 18651-18654 DT denotes the
T4550 18655-18667 NNS denotes preparations
T4551 18668-18672 VBD denotes were
T4548 18673-18677 VBN denotes made
T4553 18678-18682 IN denotes from
T4554 18683-18688 JJ denotes whole
T4555 18689-18695 NNS denotes testes
T4556 18695-18697 , denotes ,
T4557 18697-18699 PRP denotes it
T4552 18700-18702 VBZ denotes is
T4558 18703-18711 JJ denotes possible
T4559 18712-18716 IN denotes that
T4561 18717-18720 DT denotes the
T4563 18721-18730 NN denotes univalent
T4565 18730-18731 HYPH denotes -
T4564 18731-18741 VBG denotes containing
T4562 18742-18752 NNS denotes metaphases
T4566 18753-18757 IN denotes from
T4567 18758-18762 NN denotes Dmc1
T4569 18763-18764 SYM denotes −
T4570 18764-18765 HYPH denotes /
T4571 18765-18766 SYM denotes −
T4568 18767-18771 NNS denotes mice
T4560 18772-18776 VBD denotes were
T4572 18777-18781 IN denotes from
T4573 18782-18795 NNS denotes spermatogonia
T4574 18795-18797 , denotes ,
T4575 18797-18800 CC denotes not
T4576 18801-18814 NNS denotes spermatocytes
T4577 18814-18815 . denotes .
T4919 18817-18823 NN denotes TRIP13
T4920 18824-18834 NN denotes Deficiency
T4922 18835-18839 VBP denotes Does
T4923 18840-18843 RB denotes Not
T4921 18844-18853 VB denotes Alleviate
T4924 18854-18861 JJ denotes Meiotic
T4925 18862-18868 NN denotes Arrest
T4926 18869-18879 NNS denotes Phenotypes
T4927 18880-18882 IN denotes of
T4928 18883-18890 NNS denotes Mutants
T4929 18891-18900 JJ denotes Defective
T4930 18901-18903 IN denotes in
T4931 18904-18912 NN denotes Synapsis
T4932 18912-19069 sentence denotes To determine if TRIP13 deficiency prevents apoptosis triggered by asynapsis as in C. elegans, we analyzed mice that were doubly mutant for Spo11 and Trip13.
T4933 18913-18915 TO denotes To
T4934 18916-18925 VB denotes determine
T4936 18926-18928 IN denotes if
T4938 18929-18935 NN denotes TRIP13
T4939 18936-18946 NN denotes deficiency
T4937 18947-18955 VBZ denotes prevents
T4940 18956-18965 NN denotes apoptosis
T4941 18966-18975 VBN denotes triggered
T4942 18976-18978 IN denotes by
T4943 18979-18988 NN denotes asynapsis
T4944 18989-18991 IN denotes as
T4945 18992-18994 IN denotes in
T4946 18995-18997 NNP denotes C.
T4947 18998-19005 NNP denotes elegans
T4948 19005-19007 , denotes ,
T4949 19007-19009 PRP denotes we
T4935 19010-19018 VBD denotes analyzed
T4950 19019-19023 NNS denotes mice
T4951 19024-19028 WDT denotes that
T4952 19029-19033 VBD denotes were
T4953 19034-19040 RB denotes doubly
T4954 19041-19047 NN denotes mutant
T4955 19048-19051 IN denotes for
T4956 19052-19057 NN denotes Spo11
T4957 19058-19061 CC denotes and
T4958 19062-19068 NN denotes Trip13
T4959 19068-19069 . denotes .
T4960 19069-19216 sentence denotes SPO11 is a transesterase that is essential for the creation of genetically programmed DSB during leptonema of many organisms, including mice [18].
T4961 19070-19075 NN denotes SPO11
T4962 19076-19078 VBZ denotes is
T4963 19079-19080 DT denotes a
T4964 19081-19094 NN denotes transesterase
T4965 19095-19099 WDT denotes that
T4966 19100-19102 VBZ denotes is
T4967 19103-19112 JJ denotes essential
T4968 19113-19116 IN denotes for
T4969 19117-19120 DT denotes the
T4970 19121-19129 NN denotes creation
T4971 19130-19132 IN denotes of
T4972 19133-19144 RB denotes genetically
T4973 19145-19155 VBN denotes programmed
T4974 19156-19159 NN denotes DSB
T4975 19160-19166 IN denotes during
T4976 19167-19176 NN denotes leptonema
T4977 19177-19179 IN denotes of
T4978 19180-19184 JJ denotes many
T4979 19185-19194 NNS denotes organisms
T4980 19194-19196 , denotes ,
T4981 19196-19205 VBG denotes including
T4982 19206-19210 NNS denotes mice
T4983 19211-19212 -LRB- denotes [
T4984 19212-19214 CD denotes 18
T4985 19214-19215 -RRB- denotes ]
T4986 19215-19216 . denotes .
T4987 19216-19338 sentence denotes In C. elegans, spo-11 mutant gametes have extensive asynapsis, which triggers PCH-2 dependent apoptosis in pachynema [2].
T4988 19217-19219 IN denotes In
T4990 19220-19222 NNP denotes C.
T4991 19223-19230 NNP denotes elegans
T4992 19230-19232 , denotes ,
T4993 19232-19235 NN denotes spo
T4995 19235-19236 HYPH denotes -
T4996 19236-19238 CD denotes 11
T4997 19239-19245 NN denotes mutant
T4994 19246-19253 NNS denotes gametes
T4989 19254-19258 VBP denotes have
T4998 19259-19268 JJ denotes extensive
T4999 19269-19278 NN denotes asynapsis
T5000 19278-19280 , denotes ,
T5001 19280-19285 WDT denotes which
T5002 19286-19294 VBZ denotes triggers
T5003 19295-19298 NN denotes PCH
T5005 19298-19299 HYPH denotes -
T5006 19299-19300 CD denotes 2
T5004 19301-19310 JJ denotes dependent
T5007 19311-19320 NN denotes apoptosis
T5008 19321-19323 IN denotes in
T5009 19324-19333 NN denotes pachynema
T5010 19334-19335 -LRB- denotes [
T5011 19335-19336 CD denotes 2
T5012 19336-19337 -RRB- denotes ]
T5013 19337-19338 . denotes .
T5014 19338-19541 sentence denotes In mice, Spo11 −/− spermatocytes are severely defective in homologous chromosome synapsis [34,35], and arrest with chromosomes in a state characteristic of the zygotene/pachytene transition (Figure 3H).
T5015 19339-19341 IN denotes In
T5017 19342-19346 NNS denotes mice
T5018 19346-19348 , denotes ,
T5019 19348-19353 NN denotes Spo11
T5021 19354-19355 SYM denotes −
T5022 19355-19356 HYPH denotes /
T5023 19356-19357 SYM denotes −
T5020 19358-19371 NNS denotes spermatocytes
T5016 19372-19375 VBP denotes are
T5024 19376-19384 RB denotes severely
T5025 19385-19394 JJ denotes defective
T5026 19395-19397 IN denotes in
T5027 19398-19408 JJ denotes homologous
T5029 19409-19419 NN denotes chromosome
T5028 19420-19428 NN denotes synapsis
T5030 19429-19430 -LRB- denotes [
T5032 19430-19432 CD denotes 34
T5033 19432-19433 , denotes ,
T5031 19433-19435 CD denotes 35
T5034 19435-19436 -RRB- denotes ]
T5035 19436-19438 , denotes ,
T5036 19438-19441 CC denotes and
T5037 19442-19448 VBP denotes arrest
T5038 19449-19453 IN denotes with
T5039 19454-19465 NNS denotes chromosomes
T5040 19466-19468 IN denotes in
T5041 19469-19470 DT denotes a
T5042 19471-19476 NN denotes state
T5043 19477-19491 JJ denotes characteristic
T5044 19492-19494 IN denotes of
T5045 19495-19498 DT denotes the
T5047 19499-19507 NN denotes zygotene
T5049 19507-19508 HYPH denotes /
T5048 19508-19517 NN denotes pachytene
T5046 19518-19528 NN denotes transition
T5050 19529-19530 -LRB- denotes (
T5052 19530-19536 NN denotes Figure
T5051 19537-19539 NN denotes 3H
T5053 19539-19540 -RRB- denotes )
T5054 19540-19541 . denotes .
T5055 19541-19739 sentence denotes Spermatocytes in Trip13Gt/Gt Spo11 −/− testes progressed maximally to that point before undergoing death (Figs 3I), well before the spindle checkpoint that eliminates achiasmate spermatocytes [36].
T5056 19542-19555 NNS denotes Spermatocytes
T5058 19556-19558 IN denotes in
T5059 19559-19567 NN denotes Trip13Gt
T5061 19567-19568 HYPH denotes /
T5060 19568-19570 NN denotes Gt
T5063 19571-19576 NN denotes Spo11
T5064 19577-19578 SYM denotes −
T5065 19578-19579 HYPH denotes /
T5066 19579-19580 SYM denotes −
T5062 19581-19587 NNS denotes testes
T5057 19588-19598 VBD denotes progressed
T5067 19599-19608 RB denotes maximally
T5068 19609-19611 IN denotes to
T5069 19612-19616 DT denotes that
T5070 19617-19622 NN denotes point
T5071 19623-19629 IN denotes before
T5072 19630-19640 VBG denotes undergoing
T5073 19641-19646 NN denotes death
T5074 19647-19648 -LRB- denotes (
T5076 19648-19652 NNS denotes Figs
T5075 19653-19655 NN denotes 3I
T5077 19655-19656 -RRB- denotes )
T5078 19656-19658 , denotes ,
T5079 19658-19662 RB denotes well
T5080 19663-19669 IN denotes before
T5081 19670-19673 DT denotes the
T5083 19674-19681 NN denotes spindle
T5082 19682-19692 NN denotes checkpoint
T5084 19693-19697 WDT denotes that
T5085 19698-19708 VBZ denotes eliminates
T5086 19709-19719 NN denotes achiasmate
T5087 19720-19733 NNS denotes spermatocytes
T5088 19734-19735 -LRB- denotes [
T5089 19735-19737 CD denotes 36
T5090 19737-19738 -RRB- denotes ]
T5091 19738-19739 . denotes .
T5092 19739-19889 sentence denotes There was no evidence of metaphase I spermatocytes or postmeiotic spermatids in these testes, unlike those seen in Trip13 single mutants (Figure 3G).
T5093 19740-19745 EX denotes There
T5094 19746-19749 VBD denotes was
T5095 19750-19752 DT denotes no
T5096 19753-19761 NN denotes evidence
T5097 19762-19764 IN denotes of
T5098 19765-19774 NN denotes metaphase
T5100 19775-19776 CD denotes I
T5099 19777-19790 NNS denotes spermatocytes
T5101 19791-19793 CC denotes or
T5102 19794-19805 JJ denotes postmeiotic
T5103 19806-19816 NNS denotes spermatids
T5104 19817-19819 IN denotes in
T5105 19820-19825 DT denotes these
T5106 19826-19832 NNS denotes testes
T5107 19832-19834 , denotes ,
T5108 19834-19840 IN denotes unlike
T5109 19841-19846 DT denotes those
T5110 19847-19851 VBN denotes seen
T5111 19852-19854 IN denotes in
T5112 19855-19861 NN denotes Trip13
T5114 19862-19868 JJ denotes single
T5113 19869-19876 NNS denotes mutants
T5115 19877-19878 -LRB- denotes (
T5117 19878-19884 NN denotes Figure
T5116 19885-19887 NN denotes 3G
T5118 19887-19888 -RRB- denotes )
T5119 19888-19889 . denotes .
T5120 19889-20229 sentence denotes In contrast to the complete synapsis in Trip13Gt/Gt pachytene spermatocytes (Figure 5A), in which SPO11 is available in leptonema to initiate (via DSB induction, Figure S2A and S2B) a recombination-driven homolog search, chromosome synapsis in doubly mutant spermatocytes was highly disrupted as in Spo11 single mutants (Figure 5B and 5C).
T5121 19890-19892 IN denotes In
T5123 19893-19901 NN denotes contrast
T5124 19902-19904 IN denotes to
T5125 19905-19908 DT denotes the
T5127 19909-19917 JJ denotes complete
T5126 19918-19926 NN denotes synapsis
T5128 19927-19929 IN denotes in
T5129 19930-19938 NN denotes Trip13Gt
T5131 19938-19939 HYPH denotes /
T5130 19939-19941 NN denotes Gt
T5132 19942-19951 NN denotes pachytene
T5133 19952-19965 NNS denotes spermatocytes
T5134 19966-19967 -LRB- denotes (
T5136 19967-19973 NN denotes Figure
T5135 19974-19976 NN denotes 5A
T5137 19976-19977 -RRB- denotes )
T5138 19977-19979 , denotes ,
T5139 19979-19981 IN denotes in
T5141 19982-19987 WDT denotes which
T5142 19988-19993 NN denotes SPO11
T5140 19994-19996 VBZ denotes is
T5143 19997-20006 JJ denotes available
T5144 20007-20009 IN denotes in
T5145 20010-20019 NN denotes leptonema
T5146 20020-20022 TO denotes to
T5147 20023-20031 VB denotes initiate
T5148 20032-20033 -LRB- denotes (
T5150 20033-20036 IN denotes via
T5151 20037-20040 NN denotes DSB
T5152 20041-20050 NN denotes induction
T5153 20050-20052 , denotes ,
T5154 20052-20058 NN denotes Figure
T5149 20059-20062 NN denotes S2A
T5155 20063-20066 CC denotes and
T5156 20067-20070 NN denotes S2B
T5157 20070-20071 -RRB- denotes )
T5158 20072-20073 DT denotes a
T5160 20074-20087 NN denotes recombination
T5162 20087-20088 HYPH denotes -
T5161 20088-20094 VBN denotes driven
T5163 20095-20102 NN denotes homolog
T5159 20103-20109 NN denotes search
T5164 20109-20111 , denotes ,
T5165 20111-20121 NN denotes chromosome
T5166 20122-20130 NN denotes synapsis
T5167 20131-20133 IN denotes in
T5168 20134-20140 RB denotes doubly
T5169 20141-20147 JJ denotes mutant
T5170 20148-20161 NNS denotes spermatocytes
T5171 20162-20165 VBD denotes was
T5172 20166-20172 RB denotes highly
T5122 20173-20182 VBN denotes disrupted
T5173 20183-20185 IN denotes as
T5174 20186-20188 IN denotes in
T5175 20189-20194 NN denotes Spo11
T5177 20195-20201 JJ denotes single
T5176 20202-20209 NNS denotes mutants
T5178 20210-20211 -LRB- denotes (
T5180 20211-20217 NN denotes Figure
T5179 20218-20220 NN denotes 5B
T5181 20221-20224 CC denotes and
T5182 20225-20227 NN denotes 5C
T5183 20227-20228 -RRB- denotes )
T5184 20228-20229 . denotes .
T5185 20229-20448 sentence denotes Identical studies were performed with mice deficient for Mei1, a vertebrate-specific gene also required for DSB formation and chromosome synapsis [37], with similar results (Figure 3J and 3K; immunocytology not shown).
T5186 20230-20239 JJ denotes Identical
T5187 20240-20247 NNS denotes studies
T5189 20248-20252 VBD denotes were
T5188 20253-20262 VBN denotes performed
T5190 20263-20267 IN denotes with
T5191 20268-20272 NNS denotes mice
T5192 20273-20282 JJ denotes deficient
T5193 20283-20286 IN denotes for
T5194 20287-20291 NN denotes Mei1
T5195 20291-20293 , denotes ,
T5196 20293-20294 DT denotes a
T5198 20295-20305 NN denotes vertebrate
T5200 20305-20306 HYPH denotes -
T5199 20306-20314 JJ denotes specific
T5197 20315-20319 NN denotes gene
T5201 20320-20324 RB denotes also
T5202 20325-20333 VBN denotes required
T5203 20334-20337 IN denotes for
T5204 20338-20341 NN denotes DSB
T5205 20342-20351 NN denotes formation
T5206 20352-20355 CC denotes and
T5207 20356-20366 NN denotes chromosome
T5208 20367-20375 NN denotes synapsis
T5209 20376-20377 -LRB- denotes [
T5210 20377-20379 CD denotes 37
T5211 20379-20380 -RRB- denotes ]
T5212 20380-20382 , denotes ,
T5213 20382-20386 IN denotes with
T5214 20387-20394 JJ denotes similar
T5215 20395-20402 NNS denotes results
T5216 20403-20404 -LRB- denotes (
T5218 20404-20410 NN denotes Figure
T5217 20411-20413 NN denotes 3J
T5219 20414-20417 CC denotes and
T5220 20418-20420 NN denotes 3K
T5221 20420-20421 : denotes ;
T5222 20422-20436 NN denotes immunocytology
T5224 20437-20440 RB denotes not
T5223 20441-20446 VBN denotes shown
T5225 20446-20447 -RRB- denotes )
T5226 20447-20448 . denotes .
T5227 20448-20449 sentence denotes
T12126 20459-20476 JJ denotes Immunocytological
T12127 20477-20485 NN denotes Analysis
T12128 20486-20488 IN denotes of
T12129 20489-20495 NN denotes Trip13
T12131 20496-20504 NN denotes Compound
T12130 20505-20512 NNS denotes Mutants
T12132 20512-20606 sentence denotes Surface-spread chromosomes were immunolabeled with the indicated antibodies and fluorophores.
T12133 20513-20520 NN denotes Surface
T12135 20520-20521 HYPH denotes -
T12134 20521-20527 NN denotes spread
T12136 20528-20539 NNS denotes chromosomes
T12138 20540-20544 VBD denotes were
T12137 20545-20558 VBN denotes immunolabeled
T12139 20559-20563 IN denotes with
T12140 20564-20567 DT denotes the
T12142 20568-20577 VBN denotes indicated
T12141 20578-20588 NNS denotes antibodies
T12143 20589-20592 CC denotes and
T12144 20593-20605 NNS denotes fluorophores
T12145 20605-20606 . denotes .
T12146 20606-20695 sentence denotes Genotypes are indicated, as are those panels in which dual staining patterns are merged.
T12147 20607-20616 NNS denotes Genotypes
T12149 20617-20620 VBP denotes are
T12148 20621-20630 VBN denotes indicated
T12150 20630-20632 , denotes ,
T12151 20632-20634 IN denotes as
T12152 20635-20638 VBP denotes are
T12153 20639-20644 DT denotes those
T12154 20645-20651 NNS denotes panels
T12155 20652-20654 IN denotes in
T12157 20655-20660 WDT denotes which
T12158 20661-20665 JJ denotes dual
T12160 20666-20674 NN denotes staining
T12159 20675-20683 NNS denotes patterns
T12161 20684-20687 VBP denotes are
T12156 20688-20694 VBN denotes merged
T12162 20694-20695 . denotes .
T12163 20695-20769 sentence denotes Note that (H) and (I) are at lower magnification to show multiple nuclei.
T12164 20696-20700 VB denotes Note
T12165 20701-20705 IN denotes that
T12167 20706-20707 -LRB- denotes (
T12168 20707-20708 NN denotes H
T12169 20708-20709 -RRB- denotes )
T12170 20710-20713 CC denotes and
T12171 20714-20715 -LRB- denotes (
T12172 20715-20716 NN denotes I
T12173 20716-20717 -RRB- denotes )
T12166 20718-20721 VBP denotes are
T12174 20722-20724 IN denotes at
T12175 20725-20730 JJR denotes lower
T12176 20731-20744 NN denotes magnification
T12177 20745-20747 TO denotes to
T12178 20748-20752 VB denotes show
T12179 20753-20761 JJ denotes multiple
T12180 20762-20768 NNS denotes nuclei
T12181 20768-20769 . denotes .
T5229 20770-20772 JJ denotes In
T5228 20770-20774 sentence denotes In y
T5230 20773-20774 NN denotes y
T5232 20774-20775 NN denotes e
T5231 20774-20778 sentence denotes east
T5233 20775-20776 NN denotes a
T5234 20776-20777 NNS denotes s
T5235 20777-20778 IN denotes t
T5237 20778-20780 , denotes ,
T5236 20778-20782 sentence denotes , de
T5238 20780-20781 JJ denotes d
T5239 20781-20782 NNS denotes e
T5241 20782-20784 JJR denotes le
T5240 20782-20790 sentence denotes letion o
T5242 20784-20788 NN denotes tion
T5243 20789-20790 TO denotes o
T5246 20790-20791 IN denotes f
T5244 20790-20875 sentence denotes f PCH2 alleviates the pachytene arrest caused by asynaptic mutants zip1 and zip2 [8].
T5247 20792-20796 NN denotes PCH2
T5245 20797-20807 VBZ denotes alleviates
T5248 20808-20811 DT denotes the
T5250 20812-20821 NN denotes pachytene
T5249 20822-20828 NN denotes arrest
T5251 20829-20835 VBN denotes caused
T5252 20836-20838 IN denotes by
T5253 20839-20848 JJ denotes asynaptic
T5255 20849-20856 NNS denotes mutants
T5254 20857-20861 NN denotes zip1
T5256 20862-20865 CC denotes and
T5257 20866-20870 NN denotes zip2
T5258 20871-20872 -LRB- denotes [
T5259 20872-20873 CD denotes 8
T5260 20873-20874 -RRB- denotes ]
T5261 20874-20875 . denotes .
T5262 20875-21094 sentence denotes Although mouse SYCP1 might be a functional equivalent of Zip1p, because Sycp1 mutant spermatocytes arrest at approximately the same point as Trip13 mutants, there would be no opportunity to observe bypass of Sycp1 −/−.
T5263 20876-20884 IN denotes Although
T5265 20885-20890 NN denotes mouse
T5266 20891-20896 NN denotes SYCP1
T5267 20897-20902 MD denotes might
T5264 20903-20905 VB denotes be
T5269 20906-20907 DT denotes a
T5271 20908-20918 JJ denotes functional
T5270 20919-20929 NN denotes equivalent
T5272 20930-20932 IN denotes of
T5273 20933-20938 NN denotes Zip1p
T5274 20938-20940 , denotes ,
T5275 20940-20947 IN denotes because
T5277 20948-20953 NN denotes Sycp1
T5279 20954-20960 NN denotes mutant
T5278 20961-20974 NNS denotes spermatocytes
T5276 20975-20981 VBP denotes arrest
T5280 20982-20984 IN denotes at
T5281 20985-20998 RB denotes approximately
T5283 20999-21002 DT denotes the
T5284 21003-21007 JJ denotes same
T5282 21008-21013 NN denotes point
T5285 21014-21016 IN denotes as
T5286 21017-21023 NN denotes Trip13
T5287 21024-21031 NNS denotes mutants
T5288 21031-21033 , denotes ,
T5289 21033-21038 EX denotes there
T5290 21039-21044 MD denotes would
T5268 21045-21047 VB denotes be
T5291 21048-21050 DT denotes no
T5292 21051-21062 NN denotes opportunity
T5293 21063-21065 TO denotes to
T5294 21066-21073 VB denotes observe
T5295 21074-21080 NN denotes bypass
T5296 21081-21083 IN denotes of
T5297 21084-21089 NN denotes Sycp1
T5298 21090-21091 SYM denotes −
T5299 21091-21092 HYPH denotes /
T5300 21092-21093 SYM denotes −
T5301 21093-21094 . denotes .
T5302 21094-21253 sentence denotes Since Zip2p is present at sites of axial associations, even in zip1 mutants, it has been suggested that Zip2p promotes initiation of chromosome synapsis [38].
T5303 21095-21100 IN denotes Since
T5305 21101-21106 NN denotes Zip2p
T5304 21107-21109 VBZ denotes is
T5307 21110-21117 JJ denotes present
T5308 21118-21120 IN denotes at
T5309 21121-21126 NNS denotes sites
T5310 21127-21129 IN denotes of
T5311 21130-21135 JJ denotes axial
T5312 21136-21148 NNS denotes associations
T5313 21148-21150 , denotes ,
T5314 21150-21154 RB denotes even
T5315 21155-21157 IN denotes in
T5316 21158-21162 NN denotes zip1
T5317 21163-21170 NNS denotes mutants
T5318 21170-21172 , denotes ,
T5319 21172-21174 PRP denotes it
T5320 21175-21178 VBZ denotes has
T5321 21179-21183 VBN denotes been
T5306 21184-21193 VBN denotes suggested
T5322 21194-21198 IN denotes that
T5324 21199-21204 NN denotes Zip2p
T5323 21205-21213 VBZ denotes promotes
T5325 21214-21224 NN denotes initiation
T5326 21225-21227 IN denotes of
T5327 21228-21238 NN denotes chromosome
T5328 21239-21247 NN denotes synapsis
T5329 21248-21249 -LRB- denotes [
T5330 21249-21251 CD denotes 38
T5331 21251-21252 -RRB- denotes ]
T5332 21252-21253 . denotes .
T5333 21253-21375 sentence denotes These observations raise the possibility that in yeast, Pch2p responds to synapsis polymerization rather than initiation.
T5334 21254-21259 DT denotes These
T5335 21260-21272 NNS denotes observations
T5336 21273-21278 VBP denotes raise
T5337 21279-21282 DT denotes the
T5338 21283-21294 NN denotes possibility
T5339 21295-21299 IN denotes that
T5341 21300-21302 IN denotes in
T5342 21303-21308 NN denotes yeast
T5343 21308-21310 , denotes ,
T5344 21310-21315 NN denotes Pch2p
T5340 21316-21324 VBZ denotes responds
T5345 21325-21327 IN denotes to
T5346 21328-21336 NN denotes synapsis
T5347 21337-21351 NN denotes polymerization
T5348 21352-21358 RB denotes rather
T5349 21359-21363 IN denotes than
T5350 21364-21374 NN denotes initiation
T5351 21374-21375 . denotes .
T5352 21375-21475 sentence denotes To test this, we performed epistasis analysis with a Rec8 allele (Rec8Mei8, abbreviated as Rec8 −).
T5353 21376-21378 TO denotes To
T5354 21379-21383 VB denotes test
T5356 21384-21388 DT denotes this
T5357 21388-21390 , denotes ,
T5358 21390-21392 PRP denotes we
T5355 21393-21402 VBD denotes performed
T5359 21403-21412 NN denotes epistasis
T5360 21413-21421 NN denotes analysis
T5361 21422-21426 IN denotes with
T5362 21427-21428 DT denotes a
T5364 21429-21433 NN denotes Rec8
T5363 21434-21440 NN denotes allele
T5365 21441-21442 -LRB- denotes (
T5366 21442-21450 NN denotes Rec8Mei8
T5367 21450-21452 , denotes ,
T5368 21452-21463 VBN denotes abbreviated
T5369 21464-21466 IN denotes as
T5370 21467-21471 NN denotes Rec8
T5371 21472-21473 SYM denotes −
T5372 21473-21474 -RRB- denotes )
T5373 21474-21475 . denotes .
T5374 21475-21679 sentence denotes Meiotic chromosomes of Rec8 mutant spermatocytes undergo apparent homolog pairing and interhomolog synaptic initiation, but are defective in DSB repair and fail to maintain interhomolog synapsis [39,40].
T5375 21476-21483 JJ denotes Meiotic
T5376 21484-21495 NNS denotes chromosomes
T5378 21496-21498 IN denotes of
T5379 21499-21503 NN denotes Rec8
T5381 21504-21510 NN denotes mutant
T5380 21511-21524 NNS denotes spermatocytes
T5377 21525-21532 VBP denotes undergo
T5382 21533-21541 JJ denotes apparent
T5384 21542-21549 NN denotes homolog
T5383 21550-21557 NN denotes pairing
T5385 21558-21561 CC denotes and
T5386 21562-21574 JJ denotes interhomolog
T5388 21575-21583 JJ denotes synaptic
T5387 21584-21594 NN denotes initiation
T5389 21594-21596 , denotes ,
T5390 21596-21599 CC denotes but
T5391 21600-21603 VBP denotes are
T5392 21604-21613 JJ denotes defective
T5393 21614-21616 IN denotes in
T5394 21617-21620 NN denotes DSB
T5395 21621-21627 NN denotes repair
T5396 21628-21631 CC denotes and
T5397 21632-21636 VBP denotes fail
T5398 21637-21639 TO denotes to
T5399 21640-21648 VB denotes maintain
T5400 21649-21661 JJ denotes interhomolog
T5401 21662-21670 NN denotes synapsis
T5402 21671-21672 -LRB- denotes [
T5404 21672-21674 CD denotes 39
T5405 21674-21675 , denotes ,
T5403 21675-21677 CD denotes 40
T5406 21677-21678 -RRB- denotes ]
T5407 21678-21679 . denotes .
T5408 21679-21764 sentence denotes Rather, sister chromatids appear to synapse and are bound by SYCP1 along their axes.
T5409 21680-21686 RB denotes Rather
T5411 21686-21688 , denotes ,
T5412 21688-21694 NN denotes sister
T5413 21695-21705 NNS denotes chromatids
T5410 21706-21712 VBP denotes appear
T5414 21713-21715 TO denotes to
T5415 21716-21723 VB denotes synapse
T5416 21724-21727 CC denotes and
T5417 21728-21731 VBP denotes are
T5418 21732-21737 VBN denotes bound
T5419 21738-21740 IN denotes by
T5420 21741-21746 NN denotes SYCP1
T5421 21747-21752 IN denotes along
T5422 21753-21758 PRP$ denotes their
T5423 21759-21763 NNS denotes axes
T5424 21763-21764 . denotes .
T5425 21764-21822 sentence denotes Rec8 mutants do not progress to diplonema or metaphase I.
T5426 21765-21769 NN denotes Rec8
T5427 21770-21777 NNS denotes mutants
T5429 21778-21780 VBP denotes do
T5430 21781-21784 RB denotes not
T5428 21785-21793 VB denotes progress
T5431 21794-21796 IN denotes to
T5432 21797-21806 NN denotes diplonema
T5433 21807-21809 CC denotes or
T5434 21810-21819 NN denotes metaphase
T5435 21820-21821 CD denotes I
T5436 21821-21822 . denotes .
T5437 21822-21889 sentence denotes Double mutant analysis indicated that Rec8 is epistatic to Trip13.
T5438 21823-21829 JJ denotes Double
T5440 21830-21836 NN denotes mutant
T5439 21837-21845 NN denotes analysis
T5441 21846-21855 VBD denotes indicated
T5442 21856-21860 IN denotes that
T5444 21861-21865 NN denotes Rec8
T5443 21866-21868 VBZ denotes is
T5445 21869-21878 JJ denotes epistatic
T5446 21879-21881 IN denotes to
T5447 21882-21888 NN denotes Trip13
T5448 21888-21889 . denotes .
T5449 21889-22108 sentence denotes As in the Spo11 and Mei1 experiments, histology of testes deficient for both REC8 and TRIP13 resembled the Rec8 mutant, with no evidence of progression to metaphase I that occurs in Trip13Gt/Gt mice (Figure 3L and 3M).
T5450 21890-21892 IN denotes As
T5452 21893-21895 IN denotes in
T5453 21896-21899 DT denotes the
T5455 21900-21905 NN denotes Spo11
T5456 21906-21909 CC denotes and
T5457 21910-21914 NN denotes Mei1
T5454 21915-21926 NNS denotes experiments
T5458 21926-21928 , denotes ,
T5459 21928-21937 NN denotes histology
T5460 21938-21940 IN denotes of
T5461 21941-21947 NNS denotes testes
T5462 21948-21957 JJ denotes deficient
T5463 21958-21961 IN denotes for
T5464 21962-21966 CC denotes both
T5465 21967-21971 NN denotes REC8
T5466 21972-21975 CC denotes and
T5467 21976-21982 NN denotes TRIP13
T5451 21983-21992 VBD denotes resembled
T5468 21993-21996 DT denotes the
T5470 21997-22001 NN denotes Rec8
T5469 22002-22008 NN denotes mutant
T5471 22008-22010 , denotes ,
T5472 22010-22014 IN denotes with
T5473 22015-22017 DT denotes no
T5474 22018-22026 NN denotes evidence
T5475 22027-22029 IN denotes of
T5476 22030-22041 NN denotes progression
T5477 22042-22044 IN denotes to
T5478 22045-22054 NN denotes metaphase
T5479 22055-22056 CD denotes I
T5480 22057-22061 WDT denotes that
T5481 22062-22068 VBZ denotes occurs
T5482 22069-22071 IN denotes in
T5483 22072-22080 NN denotes Trip13Gt
T5485 22080-22081 HYPH denotes /
T5484 22081-22083 NN denotes Gt
T5486 22084-22088 NNS denotes mice
T5487 22089-22090 -LRB- denotes (
T5489 22090-22096 NN denotes Figure
T5488 22097-22099 NN denotes 3L
T5490 22100-22103 CC denotes and
T5491 22104-22106 NN denotes 3M
T5492 22106-22107 -RRB- denotes )
T5493 22107-22108 . denotes .
T5494 22108-22337 sentence denotes Immunocytological analysis of spread chromosomes showed a failure of homologous chromosome synapsis in both the Rec8 −/− and Rec8 −/− Trip13Gt/Gt spermatocytes, as previously reported for Rec8 mutants (Figure 5D and 5E) [39,40].
T5495 22109-22126 JJ denotes Immunocytological
T5496 22127-22135 NN denotes analysis
T5498 22136-22138 IN denotes of
T5499 22139-22145 NN denotes spread
T5500 22146-22157 NNS denotes chromosomes
T5497 22158-22164 VBD denotes showed
T5501 22165-22166 DT denotes a
T5502 22167-22174 NN denotes failure
T5503 22175-22177 IN denotes of
T5504 22178-22188 JJ denotes homologous
T5506 22189-22199 NN denotes chromosome
T5505 22200-22208 NN denotes synapsis
T5507 22209-22211 IN denotes in
T5508 22212-22216 CC denotes both
T5510 22217-22220 DT denotes the
T5511 22221-22225 NN denotes Rec8
T5512 22226-22227 SYM denotes −
T5513 22227-22228 HYPH denotes /
T5514 22228-22229 SYM denotes −
T5515 22230-22233 CC denotes and
T5516 22234-22238 NN denotes Rec8
T5518 22239-22240 SYM denotes −
T5519 22240-22241 HYPH denotes /
T5520 22241-22242 SYM denotes −
T5521 22243-22251 NN denotes Trip13Gt
T5522 22251-22252 HYPH denotes /
T5517 22252-22254 NN denotes Gt
T5509 22255-22268 NNS denotes spermatocytes
T5523 22268-22270 , denotes ,
T5524 22270-22272 IN denotes as
T5526 22273-22283 RB denotes previously
T5525 22284-22292 VBN denotes reported
T5527 22293-22296 IN denotes for
T5528 22297-22301 NN denotes Rec8
T5529 22302-22309 NNS denotes mutants
T5530 22310-22311 -LRB- denotes (
T5532 22311-22317 NN denotes Figure
T5531 22318-22320 NN denotes 5D
T5533 22321-22324 CC denotes and
T5534 22325-22327 NN denotes 5E
T5535 22327-22328 -RRB- denotes )
T5536 22329-22330 -LRB- denotes [
T5538 22330-22332 CD denotes 39
T5539 22332-22333 , denotes ,
T5537 22333-22335 CD denotes 40
T5540 22335-22336 -RRB- denotes ]
T5541 22336-22337 . denotes .
T5542 22337-22540 sentence denotes Although subsequent reports indicate otherwise [10,12], deletion of PCH2 in yeast was originally reported to alleviate meiotic arrest caused by deficiency for the meiosis-specific RecA homolog DMC1 [8].
T5543 22338-22346 IN denotes Although
T5545 22347-22357 JJ denotes subsequent
T5546 22358-22365 NNS denotes reports
T5544 22366-22374 VBP denotes indicate
T5548 22375-22384 RB denotes otherwise
T5549 22385-22386 -LRB- denotes [
T5551 22386-22388 CD denotes 10
T5552 22388-22389 , denotes ,
T5550 22389-22391 CD denotes 12
T5553 22391-22392 -RRB- denotes ]
T5554 22392-22394 , denotes ,
T5555 22394-22402 NN denotes deletion
T5556 22403-22405 IN denotes of
T5557 22406-22410 NN denotes PCH2
T5558 22411-22413 IN denotes in
T5559 22414-22419 NN denotes yeast
T5560 22420-22423 VBD denotes was
T5561 22424-22434 RB denotes originally
T5547 22435-22443 VBN denotes reported
T5562 22444-22446 TO denotes to
T5563 22447-22456 VB denotes alleviate
T5564 22457-22464 JJ denotes meiotic
T5565 22465-22471 NN denotes arrest
T5566 22472-22478 VBN denotes caused
T5567 22479-22481 IN denotes by
T5568 22482-22492 NN denotes deficiency
T5569 22493-22496 IN denotes for
T5570 22497-22500 DT denotes the
T5572 22501-22508 NN denotes meiosis
T5574 22508-22509 HYPH denotes -
T5573 22509-22517 JJ denotes specific
T5575 22518-22522 NN denotes RecA
T5571 22523-22530 NN denotes homolog
T5576 22531-22535 NN denotes DMC1
T5577 22536-22537 -LRB- denotes [
T5578 22537-22538 CD denotes 8
T5579 22538-22539 -RRB- denotes ]
T5580 22539-22540 . denotes .
T5581 22540-22640 sentence denotes To investigate this relationship in mice, we constructed animals doubly mutant for Trip13 and Dmc1.
T5582 22541-22543 TO denotes To
T5583 22544-22555 VB denotes investigate
T5585 22556-22560 DT denotes this
T5586 22561-22573 NN denotes relationship
T5587 22574-22576 IN denotes in
T5588 22577-22581 NNS denotes mice
T5589 22581-22583 , denotes ,
T5590 22583-22585 PRP denotes we
T5584 22586-22597 VBD denotes constructed
T5591 22598-22605 NNS denotes animals
T5592 22606-22612 RB denotes doubly
T5593 22613-22619 JJ denotes mutant
T5594 22620-22623 IN denotes for
T5595 22624-22630 NN denotes Trip13
T5596 22631-22634 CC denotes and
T5597 22635-22639 NN denotes Dmc1
T5598 22639-22640 . denotes .
T5599 22640-22943 sentence denotes As in Dmc1 −/− mice, in which spermatocytes undergo meiotic arrest from defective DSB repair and failed chromosome synapsis [16], spermatogenesis in Dmc1 −/− Trip13Gt/Gt testes was uniformly arrested at the point where spermatocytes contained chromatin characteristic of zygonema/pachynema (Figure 3N).
T5600 22641-22643 IN denotes As
T5602 22644-22646 IN denotes in
T5603 22647-22651 NN denotes Dmc1
T5605 22652-22653 SYM denotes −
T5606 22653-22654 HYPH denotes /
T5607 22654-22655 SYM denotes −
T5604 22656-22660 NNS denotes mice
T5608 22660-22662 , denotes ,
T5609 22662-22664 IN denotes in
T5611 22665-22670 WDT denotes which
T5612 22671-22684 NNS denotes spermatocytes
T5610 22685-22692 VBP denotes undergo
T5613 22693-22700 JJ denotes meiotic
T5614 22701-22707 NN denotes arrest
T5615 22708-22712 IN denotes from
T5616 22713-22722 JJ denotes defective
T5618 22723-22726 NN denotes DSB
T5617 22727-22733 NN denotes repair
T5619 22734-22737 CC denotes and
T5620 22738-22744 VBN denotes failed
T5622 22745-22755 NN denotes chromosome
T5621 22756-22764 NN denotes synapsis
T5623 22765-22766 -LRB- denotes [
T5624 22766-22768 CD denotes 16
T5625 22768-22769 -RRB- denotes ]
T5626 22769-22771 , denotes ,
T5627 22771-22786 NN denotes spermatogenesis
T5628 22787-22789 IN denotes in
T5629 22790-22794 NN denotes Dmc1
T5631 22795-22796 SYM denotes −
T5632 22796-22797 HYPH denotes /
T5633 22797-22798 SYM denotes −
T5634 22799-22807 NN denotes Trip13Gt
T5636 22807-22808 HYPH denotes /
T5635 22808-22810 NN denotes Gt
T5630 22811-22817 NNS denotes testes
T5637 22818-22821 VBD denotes was
T5638 22822-22831 RB denotes uniformly
T5601 22832-22840 VBN denotes arrested
T5639 22841-22843 IN denotes at
T5640 22844-22847 DT denotes the
T5641 22848-22853 NN denotes point
T5642 22854-22859 WRB denotes where
T5644 22860-22873 NNS denotes spermatocytes
T5643 22874-22883 VBD denotes contained
T5645 22884-22893 NN denotes chromatin
T5646 22894-22908 JJ denotes characteristic
T5647 22909-22911 IN denotes of
T5648 22912-22920 NN denotes zygonema
T5650 22920-22921 HYPH denotes /
T5649 22921-22930 NN denotes pachynema
T5651 22931-22932 -LRB- denotes (
T5653 22932-22938 NN denotes Figure
T5652 22939-22941 NN denotes 3N
T5654 22941-22942 -RRB- denotes )
T5655 22942-22943 . denotes .
T5656 22943-23243 sentence denotes Immunocytological analysis indicated that both Dmc1 −/− and Dmc1 −/− Trip13Gt/Gt chromosomes had extensive asynapsis compared to Trip13Gt single mutants (Figure 5F–5H), and all had persistent RAD51/DMC1 foci and phosphorylated H2AX (γH2AX; Figure 5I–5L), confirming that Dmc1 is epistatic to Trip13.
T5657 22944-22961 JJ denotes Immunocytological
T5658 22962-22970 NN denotes analysis
T5659 22971-22980 VBD denotes indicated
T5660 22981-22985 IN denotes that
T5662 22986-22990 CC denotes both
T5663 22991-22995 NN denotes Dmc1
T5665 22996-22997 SYM denotes −
T5666 22997-22998 HYPH denotes /
T5667 22998-22999 SYM denotes −
T5668 23000-23003 CC denotes and
T5669 23004-23008 NN denotes Dmc1
T5671 23009-23010 SYM denotes −
T5672 23010-23011 HYPH denotes /
T5673 23011-23012 SYM denotes −
T5674 23013-23021 NN denotes Trip13Gt
T5675 23021-23022 HYPH denotes /
T5670 23022-23024 NN denotes Gt
T5664 23025-23036 NNS denotes chromosomes
T5661 23037-23040 VBD denotes had
T5676 23041-23050 JJ denotes extensive
T5677 23051-23060 NN denotes asynapsis
T5678 23061-23069 VBN denotes compared
T5679 23070-23072 IN denotes to
T5680 23073-23081 NN denotes Trip13Gt
T5682 23082-23088 JJ denotes single
T5681 23089-23096 NNS denotes mutants
T5683 23097-23098 -LRB- denotes (
T5685 23098-23104 NN denotes Figure
T5686 23105-23107 NN denotes 5F
T5687 23107-23108 SYM denotes –
T5684 23108-23110 NN denotes 5H
T5688 23110-23111 -RRB- denotes )
T5689 23111-23113 , denotes ,
T5690 23113-23116 CC denotes and
T5691 23117-23120 DT denotes all
T5692 23121-23124 VBD denotes had
T5693 23125-23135 JJ denotes persistent
T5695 23136-23141 NN denotes RAD51
T5697 23141-23142 HYPH denotes /
T5696 23142-23146 NN denotes DMC1
T5694 23147-23151 NNS denotes foci
T5698 23152-23155 CC denotes and
T5699 23156-23170 VBN denotes phosphorylated
T5700 23171-23175 NN denotes H2AX
T5701 23176-23177 -LRB- denotes (
T5703 23177-23182 NN denotes γH2AX
T5704 23182-23183 : denotes ;
T5705 23184-23190 NN denotes Figure
T5706 23191-23193 NN denotes 5I
T5707 23193-23194 SYM denotes –
T5702 23194-23196 NN denotes 5L
T5708 23196-23197 -RRB- denotes )
T5709 23197-23199 , denotes ,
T5710 23199-23209 VBG denotes confirming
T5711 23210-23214 IN denotes that
T5713 23215-23219 NN denotes Dmc1
T5712 23220-23222 VBZ denotes is
T5714 23223-23232 JJ denotes epistatic
T5715 23233-23235 IN denotes to
T5716 23236-23242 NN denotes Trip13
T5717 23242-23243 . denotes .
T5718 23243-23360 sentence denotes Doubly mutant females had residual ovaries, phenocopying Dmc1 −/− and Trip13Gt/Gt single mutants (unpublished data).
T5719 23244-23250 RB denotes Doubly
T5720 23251-23257 JJ denotes mutant
T5721 23258-23265 NNS denotes females
T5722 23266-23269 VBD denotes had
T5723 23270-23278 JJ denotes residual
T5724 23279-23286 NNS denotes ovaries
T5725 23286-23288 , denotes ,
T5726 23288-23300 VBG denotes phenocopying
T5727 23301-23305 NN denotes Dmc1
T5729 23306-23307 SYM denotes −
T5730 23307-23308 HYPH denotes /
T5731 23308-23309 SYM denotes −
T5732 23310-23313 CC denotes and
T5733 23314-23322 NN denotes Trip13Gt
T5735 23322-23323 HYPH denotes /
T5734 23323-23325 NN denotes Gt
T5736 23326-23332 JJ denotes single
T5728 23333-23340 NNS denotes mutants
T5737 23341-23342 -LRB- denotes (
T5739 23342-23353 JJ denotes unpublished
T5738 23354-23358 NNS denotes data
T5740 23358-23359 -RRB- denotes )
T5741 23359-23360 . denotes .
T5807 23362-23369 JJ denotes Meiotic
T5808 23370-23377 NNS denotes Defects
T5810 23378-23380 IN denotes in
T5811 23381-23389 NN denotes Trip13Gt
T5813 23389-23390 HYPH denotes /
T5812 23390-23392 NN denotes Gt
T5814 23393-23400 NNS denotes Oocytes
T5809 23401-23404 VBP denotes Are
T5815 23405-23408 NN denotes DSB
T5817 23408-23409 HYPH denotes -
T5816 23409-23418 JJ denotes Dependent
T5818 23418-23518 sentence denotes Epistasis analysis of females was insightful with respect to the cause of arrest in Trip13 mutants.
T5819 23419-23428 NN denotes Epistasis
T5820 23429-23437 NN denotes analysis
T5822 23438-23440 IN denotes of
T5823 23441-23448 NNS denotes females
T5821 23449-23452 VBD denotes was
T5824 23453-23463 JJ denotes insightful
T5825 23464-23468 IN denotes with
T5826 23469-23476 NN denotes respect
T5827 23477-23479 IN denotes to
T5828 23480-23483 DT denotes the
T5829 23484-23489 NN denotes cause
T5830 23490-23492 IN denotes of
T5831 23493-23499 NN denotes arrest
T5832 23500-23502 IN denotes in
T5833 23503-23509 NN denotes Trip13
T5834 23510-23517 NNS denotes mutants
T5835 23517-23518 . denotes .
T5836 23518-23674 sentence denotes Both Mei1 −/−/Trip13Gt/Gt and Spo11 −/−/Trip13Gt/Gt females had ovaries with numerous follicles, identical to Mei1 and Spo11 single mutants (Figure 3O–3R).
T5837 23519-23523 CC denotes Both
T5839 23524-23528 NN denotes Mei1
T5840 23529-23530 SYM denotes −
T5841 23530-23531 HYPH denotes /
T5842 23531-23532 SYM denotes −
T5843 23532-23533 HYPH denotes /
T5844 23533-23541 NN denotes Trip13Gt
T5845 23541-23542 HYPH denotes /
T5838 23542-23544 NN denotes Gt
T5847 23545-23548 CC denotes and
T5848 23549-23554 NN denotes Spo11
T5850 23555-23556 SYM denotes −
T5851 23556-23557 HYPH denotes /
T5852 23557-23558 SYM denotes −
T5853 23558-23559 HYPH denotes /
T5854 23559-23567 NN denotes Trip13Gt
T5855 23567-23568 HYPH denotes /
T5849 23568-23570 NN denotes Gt
T5856 23571-23578 NNS denotes females
T5846 23579-23582 VBD denotes had
T5857 23583-23590 NNS denotes ovaries
T5858 23591-23595 IN denotes with
T5859 23596-23604 JJ denotes numerous
T5860 23605-23614 NNS denotes follicles
T5861 23614-23616 , denotes ,
T5862 23616-23625 JJ denotes identical
T5863 23626-23628 IN denotes to
T5864 23629-23633 NN denotes Mei1
T5866 23634-23637 CC denotes and
T5867 23638-23643 NN denotes Spo11
T5868 23644-23650 JJ denotes single
T5865 23651-23658 NNS denotes mutants
T5869 23659-23660 -LRB- denotes (
T5871 23660-23666 NN denotes Figure
T5872 23667-23669 NN denotes 3O
T5873 23669-23670 SYM denotes –
T5870 23670-23672 NN denotes 3R
T5874 23672-23673 -RRB- denotes )
T5875 23673-23674 . denotes .
T5876 23674-23767 sentence denotes Thus, Spo11 and Mei1 are epistatic to Trip13 in oogenesis, just as they are to Dmc1 [13,41].
T5877 23675-23679 RB denotes Thus
T5879 23679-23681 , denotes ,
T5880 23681-23686 NN denotes Spo11
T5881 23687-23690 CC denotes and
T5882 23691-23695 NN denotes Mei1
T5878 23696-23699 VBP denotes are
T5883 23700-23709 JJ denotes epistatic
T5884 23710-23712 IN denotes to
T5885 23713-23719 NN denotes Trip13
T5886 23720-23722 IN denotes in
T5887 23723-23732 NN denotes oogenesis
T5888 23732-23734 , denotes ,
T5889 23734-23738 RB denotes just
T5891 23739-23741 IN denotes as
T5892 23742-23746 PRP denotes they
T5890 23747-23750 VBP denotes are
T5893 23751-23753 IN denotes to
T5894 23754-23758 NN denotes Dmc1
T5895 23759-23760 -LRB- denotes [
T5897 23760-23762 CD denotes 13
T5898 23762-23763 , denotes ,
T5896 23763-23765 CD denotes 41
T5899 23765-23766 -RRB- denotes ]
T5900 23766-23767 . denotes .
T5901 23767-23856 sentence denotes This demonstrates that oocyte loss in Trip13Gt/Gt females is dependent on DSB formation.
T5902 23768-23772 DT denotes This
T5903 23773-23785 VBZ denotes demonstrates
T5904 23786-23790 IN denotes that
T5906 23791-23797 NN denotes oocyte
T5907 23798-23802 NN denotes loss
T5908 23803-23805 IN denotes in
T5909 23806-23814 NN denotes Trip13Gt
T5911 23814-23815 HYPH denotes /
T5910 23815-23817 NN denotes Gt
T5912 23818-23825 NNS denotes females
T5905 23826-23828 VBZ denotes is
T5913 23829-23838 JJ denotes dependent
T5914 23839-23841 IN denotes on
T5915 23842-23845 NN denotes DSB
T5916 23846-23855 NN denotes formation
T5917 23855-23856 . denotes .
T5918 23856-24016 sentence denotes In conjunction with the immunohistochemical data, these data provide strong evidence that meiotic arrest in Trip13 mutant mice is due to defects in DSB repair.
T5919 23857-23859 IN denotes In
T5921 23860-23871 NN denotes conjunction
T5922 23872-23876 IN denotes with
T5923 23877-23880 DT denotes the
T5925 23881-23900 JJ denotes immunohistochemical
T5924 23901-23905 NNS denotes data
T5926 23905-23907 , denotes ,
T5927 23907-23912 DT denotes these
T5928 23913-23917 NNS denotes data
T5920 23918-23925 VBP denotes provide
T5929 23926-23932 JJ denotes strong
T5930 23933-23941 NN denotes evidence
T5931 23942-23946 IN denotes that
T5933 23947-23954 JJ denotes meiotic
T5934 23955-23961 NN denotes arrest
T5935 23962-23964 IN denotes in
T5936 23965-23971 NN denotes Trip13
T5938 23972-23978 NN denotes mutant
T5937 23979-23983 NNS denotes mice
T5932 23984-23986 VBZ denotes is
T5939 23987-23990 IN denotes due
T5940 23991-23993 IN denotes to
T5941 23994-24001 NNS denotes defects
T5942 24002-24004 IN denotes in
T5943 24005-24008 NN denotes DSB
T5944 24009-24015 NN denotes repair
T5945 24015-24016 . denotes .
T5946 24016-24155 sentence denotes As expected, ovaries of Rec8 Trip13 double mutants were devoid of oocytes as were those from either single mutant (Figure 3B, 3S, and 3T).
T5947 24017-24019 IN denotes As
T5948 24020-24028 VBN denotes expected
T5950 24028-24030 , denotes ,
T5951 24030-24037 NNS denotes ovaries
T5952 24038-24040 IN denotes of
T5953 24041-24045 NN denotes Rec8
T5954 24046-24052 NN denotes Trip13
T5956 24053-24059 JJ denotes double
T5955 24060-24067 NNS denotes mutants
T5949 24068-24072 VBD denotes were
T5957 24073-24079 JJ denotes devoid
T5958 24080-24082 IN denotes of
T5959 24083-24090 NNS denotes oocytes
T5960 24091-24093 IN denotes as
T5961 24094-24098 VBD denotes were
T5962 24099-24104 DT denotes those
T5963 24105-24109 IN denotes from
T5964 24110-24116 CC denotes either
T5966 24117-24123 JJ denotes single
T5965 24124-24130 NN denotes mutant
T5967 24131-24132 -LRB- denotes (
T5969 24132-24138 NN denotes Figure
T5968 24139-24141 NN denotes 3B
T5970 24141-24143 , denotes ,
T5971 24143-24145 NN denotes 3S
T5972 24145-24147 , denotes ,
T5973 24147-24150 CC denotes and
T5974 24151-24153 NN denotes 3T
T5975 24153-24154 -RRB- denotes )
T5976 24154-24155 . denotes .
R1000 T1535 T1517 punct ", ",expressed
R1001 T1536 T1517 advcl consistent,expressed
R1002 T1537 T1536 prep with,consistent
R1003 T1538 T1539 nmod mouse,EST
R1004 T1539 T1542 compound EST,data
R1005 T1540 T1538 cc and,mouse
R1006 T1541 T1538 conj human,mouse
R1007 T1542 T1537 pobj data,with
R1008 T1543 T1542 acl summarized,data
R1009 T1544 T1543 prep in,summarized
R1010 T1545 T1544 pobj Unigene,in
R1011 T1546 T1547 punct (,http://www.ncbi.nlm.nih.gov/UniGene
R1012 T1547 T1545 parataxis http://www.ncbi.nlm.nih.gov/UniGene,Unigene
R1013 T1548 T1547 punct ),http://www.ncbi.nlm.nih.gov/UniGene
R1014 T1549 T1514 punct .,showed
R1015 T1551 T1552 nsubjpass It,expressed
R1016 T1553 T1552 auxpass is,expressed
R1017 T1554 T1552 advmod also,expressed
R1018 T1555 T1552 advmod highly,expressed
R1019 T1556 T1552 prep in,expressed
R1020 T1557 T1558 amod human,mouse
R1021 T1558 T1560 compound mouse,oocytes
R1022 T1559 T1558 cc and,mouse
R1023 T1560 T1556 pobj oocytes,in
R1024 T1561 T1562 punct [,17
R1025 T1562 T1552 parataxis 17,expressed
R1026 T1563 T1562 punct ],17
R1027 T1564 T1552 punct .,expressed
R1028 T1726 T1725 prep of,Generation
R1029 T1727 T1728 compound Trip13,Mice
R1030 T1728 T1726 pobj Mice,of
R1031 T1729 T1728 compound Mutant,Mice
R1032 T1731 T1732 aux To,explore
R1033 T1732 T1733 advcl explore,generated
R1034 T1734 T1735 det the,function
R1035 T1735 T1732 dobj function,explore
R1036 T1736 T1735 prep of,function
R1037 T1737 T1736 pobj TRIP13,of
R1038 T1738 T1735 prep in,function
R1039 T1739 T1738 pobj mammals,in
R1040 T1740 T1733 punct ", ",generated
R1041 T1741 T1733 nsubj we,generated
R1042 T1742 T1733 dobj mice,generated
R1043 T1743 T1742 prep with,mice
R1044 T1744 T1745 det a,allele
R1045 T1745 T1743 pobj allele,with
R1046 T1746 T1747 compound gene,trap
R1047 T1747 T1748 npadvmod trap,disrupted
R1048 T1748 T1745 amod disrupted,allele
R1049 T1749 T1748 punct -,disrupted
R1050 T1750 T1745 punct ", ",allele
R1051 T1751 T1745 appos Trip13RRB047,allele
R1052 T1752 T1753 punct (,1C
R1053 T1753 T1733 parataxis 1C,generated
R1054 T1754 T1753 compound Figure,1C
R1055 T1755 T1753 punct ;,1C
R1056 T1756 T1753 advcl abbreviated,1C
R1057 T1757 T1756 prep as,abbreviated
R1058 T1758 T1757 pobj Trip13Gt,as
R1059 T1759 T1753 punct ),1C
R1060 T1760 T1733 punct .,generated
R1061 T1762 T1763 nsubj Heterozygotes,were
R1062 T1764 T1763 acomp normal,were
R1063 T1765 T1763 prep in,were
R1064 T1766 T1767 det all,respects
R1065 T1767 T1765 pobj respects,in
R1066 T1768 T1763 punct ", ",were
R1067 T1769 T1763 cc but,were
R1068 T1770 T1771 nsubj homozygotes,were
R1069 T1771 T1763 conj were,were
R1070 T1772 T1771 acomp present,were
R1071 T1773 T1771 prep at,were
R1072 T1774 T1775 punct ∼,3
R1073 T1775 T1778 nummod 3,ratio
R1074 T1776 T1775 quantmod 2,3
R1075 T1777 T1775 punct /,3
R1076 T1778 T1773 pobj ratio,at
R1077 T1779 T1778 det the,ratio
R1078 T1780 T1778 amod expected,ratio
R1079 T1781 T1778 prep from,ratio
R1080 T1782 T1781 pobj intercrosses,from
R1081 T1783 T1782 prep between,intercrosses
R1082 T1784 T1783 pobj heterozygotes,between
R1083 T1785 T1782 punct (,intercrosses
R1084 T1786 T1787 nummod 91,Trip13
R1085 T1787 T1782 appos Trip13,intercrosses
R1086 T1788 T1787 punct +,Trip13
R1087 T1789 T1787 punct /,Trip13
R1088 T1790 T1787 punct +,Trip13
R1089 T1791 T1787 punct ", ",Trip13
R1090 T1792 T1793 nummod 183,Trip13Gt
R1091 T1793 T1787 conj Trip13Gt,Trip13
R1092 T1794 T1793 punct /,Trip13Gt
R1093 T1795 T1793 punct +,Trip13Gt
R1094 T1796 T1793 punct ", ",Trip13Gt
R1095 T1797 T1793 cc and,Trip13Gt
R1096 T1798 T1799 nummod 61,Gt
R1097 T1799 T1793 conj Gt,Trip13Gt
R1098 T1800 T1799 compound Trip13Gt,Gt
R1099 T1801 T1799 punct /,Gt
R1100 T1802 T1771 punct ),were
R1101 T1803 T1771 punct .,were
R1102 T1805 T1806 mark Since,were
R1103 T1806 T1815 advcl were,is
R1104 T1807 T1808 punct >,90
R1105 T1808 T1809 nummod 90,%
R1106 T1809 T1806 nsubj %,were
R1107 T1810 T1809 prep of,%
R1108 T1811 T1812 amod prewean,mice
R1109 T1812 T1810 pobj mice,of
R1110 T1813 T1814 dep that,died
R1111 T1814 T1812 relcl died,mice
R1112 T1816 T1817 compound mutant,homozygotes
R1113 T1817 T1806 attr homozygotes,were
R1114 T1818 T1815 punct ", ",is
R1115 T1819 T1820 det this,discrepancy
R1116 T1820 T1815 nsubj discrepancy,is
R1117 T1821 T1815 advmod apparently,is
R1118 T1822 T1815 prep due,is
R1119 T1823 T1822 pcomp to,due
R1120 T1824 T1825 det a,lethality
R1121 T1825 T1822 pobj lethality,due
R1122 T1826 T1827 advmod partially,penetrant
R1123 T1827 T1825 amod penetrant,lethality
R1124 T1828 T1815 punct .,is
R1125 T1830 T1831 amod Most,animals
R1126 T1831 T1836 nsubj animals,were
R1127 T1832 T1831 amod surviving,animals
R1128 T1833 T1834 compound Trip13Gt,Gt
R1129 T1834 T1831 compound Gt,animals
R1130 T1835 T1834 punct /,Gt
R1131 T1837 T1838 advmod grossly,normal
R1132 T1838 T1836 acomp normal,were
R1133 T1839 T1836 punct .,were
R1134 T1841 T1842 advmod However,were
R1135 T1843 T1842 punct ", ",were
R1136 T1844 T1842 nsubj homozygotes,were
R1137 T1845 T1846 dep that,were
R1138 T1846 T1844 relcl were,homozygotes
R1139 T1847 T1846 acomp semi-congenic,were
R1140 T1848 T1849 punct (,N4
R1141 T1849 T1847 parataxis N4,semi-congenic
R1142 T1850 T1849 punct ),N4
R1143 T1851 T1846 prep on,were
R1144 T1852 T1853 det the,strain
R1145 T1853 T1851 pobj strain,on
R1146 T1854 T1855 compound C57BL,6J
R1147 T1855 T1853 compound 6J,strain
R1148 T1856 T1855 punct /,6J
R1149 T1857 T1842 advmod often,were
R1150 T1858 T1859 advmod markedly,smaller
R1151 T1859 T1842 acomp smaller,were
R1152 T1860 T1842 cc and,were
R1153 T1861 T1860 punct /,and
R1154 T1862 T1860 cc or,and
R1155 T1863 T1842 conj had,were
R1156 T1864 T1865 amod kinked,tails
R1157 T1865 T1863 dobj tails,had
R1158 T1866 T1864 cc or,kinked
R1159 T1867 T1864 conj shorter,kinked
R1160 T1868 T1869 punct (,2A
R1161 T1869 T1863 parataxis 2A,had
R1162 T1870 T1869 compound Figure,2A
R1163 T1871 T1869 cc and,2A
R1164 T1872 T1869 conj 2B,2A
R1165 T1873 T1869 punct ),2A
R1166 T1874 T1842 punct .,were
R1167 T1877 T1878 compound RT,PCR
R1168 T1878 T1880 compound PCR,analysis
R1169 T1879 T1878 punct -,PCR
R1170 T1880 T1881 nsubj analysis,revealed
R1171 T1882 T1880 prep of,analysis
R1172 T1883 T1884 compound Trip13Gt,expression
R1173 T1884 T1882 pobj expression,of
R1174 T1885 T1886 punct (,1D
R1175 T1886 T1880 parataxis 1D,analysis
R1176 T1887 T1886 compound Figure,1D
R1177 T1888 T1886 punct ),1D
R1178 T1889 T1890 det a,level
R1179 T1890 T1881 dobj level,revealed
R1180 T1891 T1890 amod low,level
R1181 T1892 T1890 prep of,level
R1182 T1893 T1894 advmod normally,spliced
R1183 T1894 T1895 amod spliced,transcripts
R1184 T1895 T1892 pobj transcripts,of
R1185 T1896 T1890 prep in,level
R1186 T1897 T1896 pobj testes,in
R1187 T1898 T1897 prep of,testes
R1188 T1899 T1898 pobj homozygotes,of
R1189 T1900 T1901 dep that,is
R1190 T1901 T1890 relcl is,level
R1191 T1902 T1901 advmod presumably,is
R1192 T1903 T1904 det a,consequence
R1193 T1904 T1901 attr consequence,is
R1194 T1905 T1904 prep of,consequence
R1195 T1906 T1907 amod incomplete,usage
R1196 T1907 T1905 pobj usage,of
R1197 T1908 T1907 prep of,usage
R1198 T1909 T1910 det the,trap
R1199 T1910 T1912 poss trap,acceptor
R1200 T1911 T1910 compound gene,trap
R1201 T1912 T1908 pobj acceptor,of
R1202 T1913 T1910 case 's,trap
R1203 T1914 T1912 compound splice,acceptor
R1204 T1915 T1881 punct .,revealed
R1205 T1917 T1918 compound Western,blot
R1206 T1918 T1919 compound blot,analysis
R1207 T1919 T1920 nsubj analysis,revealed
R1208 T1921 T1919 punct ", ",analysis
R1209 T1922 T1919 acl using,analysis
R1210 T1923 T1924 det a,antibody
R1211 T1924 T1922 dobj antibody,using
R1212 T1925 T1924 amod polyclonal,antibody
R1213 T1926 T1924 acl raised,antibody
R1214 T1927 T1926 prep against,raised
R1215 T1928 T1929 det a,peptide
R1216 T1929 T1927 pobj peptide,against
R1217 T1930 T1929 acl encoded,peptide
R1218 T1931 T1930 agent by,encoded
R1219 T1932 T1931 pobj exon,by
R1220 T1933 T1932 nummod 3,exon
R1221 T1934 T1920 punct ", ",revealed
R1222 T1935 T1936 amod multiple,species
R1223 T1936 T1920 dobj species,revealed
R1224 T1937 T1936 prep in,species
R1225 T1938 T1939 amod wild,type
R1226 T1939 T1941 nmod type,testes
R1227 T1940 T1939 punct -,type
R1228 T1941 T1937 pobj testes,in
R1229 T1942 T1939 cc and,type
R1230 T1943 T1939 conj heterozygous,type
R1231 T1944 T1936 punct ", ",species
R1232 T1945 T1946 dep one,corresponds
R1233 T1946 T1936 relcl corresponds,species
R1234 T1947 T1945 prep of,one
R1235 T1948 T1947 pobj which,of
R1236 T1949 T1946 prep to,corresponds
R1237 T1950 T1951 det the,size
R1238 T1951 T1949 pobj size,to
R1239 T1952 T1951 amod expected,size
R1240 T1953 T1951 prep of,size
R1241 T1954 T1955 nummod 48,kDa
R1242 T1955 T1953 pobj kDa,of
R1243 T1956 T1957 punct (,1E
R1244 T1957 T1920 parataxis 1E,revealed
R1245 T1958 T1957 compound Figure,1E
R1246 T1959 T1957 punct ),1E
R1247 T1960 T1920 punct .,revealed
R1248 T1962 T1963 nsubj This,were
R1249 T1964 T1962 cc and,This
R1250 T1965 T1966 nummod three,species
R1251 T1966 T1962 conj species,This
R1252 T1967 T1966 amod other,species
R1253 T1968 T1963 acomp undetectable,were
R1254 T1969 T1963 prep in,were
R1255 T1970 T1971 amod homozygous,mutant
R1256 T1971 T1972 compound mutant,testes
R1257 T1972 T1969 pobj testes,in
R1258 T1973 T1963 punct ", ",were
R1259 T1974 T1963 cc but,were
R1260 T1975 T1976 det a,amount
R1261 T1976 T1978 nsubj amount,was
R1262 T1977 T1976 amod reduced,amount
R1263 T1978 T1963 conj was,were
R1264 T1979 T1976 prep of,amount
R1265 T1980 T1981 det an,band
R1266 T1981 T1979 pobj band,of
R1267 T1982 T1981 amod intense,band
R1268 T1983 T1984 punct ∼,38
R1269 T1984 T1985 nummod 38,kDa
R1270 T1985 T1986 npadvmod kDa,smaller
R1271 T1986 T1981 amod smaller,band
R1272 T1987 T1978 acomp present,was
R1273 T1988 T1978 punct .,was
R1274 T1990 T1991 nsubj It,is
R1275 T1992 T1991 neg not,is
R1276 T1993 T1991 acomp clear,is
R1277 T1994 T1995 mark if,corresponds
R1278 T1995 T1991 ccomp corresponds,is
R1279 T1996 T1995 nsubj this,corresponds
R1280 T1997 T1995 prep to,corresponds
R1281 T1998 T1997 pobj TRIP13,to
R1282 T1999 T1991 punct .,is
R1283 T2001 T2002 det The,mRNA
R1284 T2002 T2006 nsubj mRNA,indicate
R1285 T2003 T2004 advmod greatly,decreased
R1286 T2004 T2002 amod decreased,mRNA
R1287 T2005 T2002 compound Trip13,mRNA
R1288 T2007 T2002 cc and,mRNA
R1289 T2008 T2009 amod predicted,protein
R1290 T2009 T2002 conj protein,mRNA
R1291 T2010 T2011 amod correct,length
R1292 T2011 T2009 compound length,protein
R1293 T2012 T2011 punct -,length
R1294 T2013 T2009 prep in,protein
R1295 T2014 T2013 pobj mutants,in
R1296 T2015 T2016 mark that,is
R1297 T2016 T2006 ccomp is,indicate
R1298 T2017 T2018 det the,allele
R1299 T2018 T2016 nsubj allele,is
R1300 T2019 T2018 compound Trip13RRB047,allele
R1301 T2020 T2021 advmod severely,hypomorphic
R1302 T2021 T2016 acomp hypomorphic,is
R1303 T2022 T2006 punct .,indicate
R1304 T2024 T2025 aux To,determine
R1305 T2025 T2026 advcl determine,immunolabeled
R1306 T2027 T2028 det the,types
R1307 T2028 T2025 dobj types,determine
R1308 T2029 T2030 compound germ,cell
R1309 T2030 T2028 compound cell,types
R1310 T2031 T2032 prep in,expressed
R1311 T2032 T2028 relcl expressed,types
R1312 T2033 T2031 pobj which,in
R1313 T2034 T2032 nsubjpass TRIP13,expressed
R1314 T2035 T2032 auxpass is,expressed
R1315 T2036 T2025 punct ", ",determine
R1316 T2037 T2025 cc and,determine
R1317 T2038 T2039 aux to,assess
R1318 T2039 T2025 conj assess,determine
R1319 T2040 T2041 amod possible,expression
R1320 T2041 T2039 dobj expression,assess
R1321 T2042 T2041 prep in,expression
R1322 T2043 T2044 det the,mutant
R1323 T2044 T2042 pobj mutant,in
R1324 T2045 T2039 prep by,assess
R1325 T2046 T2045 pobj means,by
R1326 T2047 T2046 amod other,means
R1327 T2048 T2047 prep than,other
R1328 T2049 T2050 compound Western,analysis
R1329 T2050 T2048 pobj analysis,than
R1330 T2051 T2026 punct ", ",immunolabeled
R1331 T2052 T2053 compound testis,sections
R1332 T2053 T2026 nsubjpass sections,immunolabeled
R1333 T2054 T2026 auxpass were,immunolabeled
R1334 T2055 T2026 prep for,immunolabeled
R1335 T2056 T2055 pobj TRIP13,for
R1336 T2057 T2026 advcl using,immunolabeled
R1337 T2058 T2059 det a,antibody
R1338 T2059 T2057 dobj antibody,using
R1339 T2060 T2061 amod polyclonal,antipeptide
R1340 T2061 T2059 compound antipeptide,antibody
R1341 T2062 T2061 compound chicken,antipeptide
R1342 T2063 T2064 punct (,see
R1343 T2064 T2026 parataxis see,immunolabeled
R1344 T2065 T2064 dobj Materials,see
R1345 T2066 T2065 cc and,Materials
R1346 T2067 T2065 conj Methods,Materials
R1347 T2068 T2064 punct ),see
R1348 T2069 T2026 punct .,immunolabeled
R1349 T2071 T2072 det The,cells
R1350 T2072 T2076 nsubj cells,were
R1351 T2073 T2074 advmod most,intensely
R1352 T2074 T2075 advmod intensely,labeled
R1353 T2075 T2072 amod labeled,cells
R1354 T2077 T2072 prep in,cells
R1355 T2078 T2079 compound control,testes
R1356 T2079 T2077 pobj testes,in
R1357 T2080 T2081 compound Type,B
R1358 T2081 T2082 compound B,spermatogonia
R1359 T2082 T2076 attr spermatogonia,were
R1360 T2083 T2082 cc and,spermatogonia
R1361 T2084 T2085 compound leptotene,spermatocytes
R1362 T2085 T2082 conj spermatocytes,spermatogonia
R1363 T2086 T2087 punct (,1F
R1364 T2087 T2076 parataxis 1F,were
R1365 T2088 T2087 compound Figure,1F
R1366 T2089 T2087 punct ),1F
R1367 T2090 T2076 punct .,were
R1368 T2092 T2093 compound Zygotene,pachytene
R1369 T2093 T2095 compound pachytene,spermatocytes
R1370 T2094 T2093 punct /,pachytene
R1371 T2095 T2096 nsubj spermatocytes,stained
R1372 T2097 T2098 advmod less,strongly
R1373 T2098 T2096 advmod strongly,stained
R1374 T2099 T2096 punct ", ",stained
R1375 T2100 T2096 cc and,stained
R1376 T2101 T2102 expl there,was
R1377 T2102 T2096 conj was,stained
R1378 T2103 T2104 det no,staining
R1379 T2104 T2102 attr staining,was
R1380 T2105 T2104 amod detectable,staining
R1381 T2106 T2104 prep in,staining
R1382 T2107 T2108 amod late,pachytene
R1383 T2108 T2109 compound pachytene,spermatocytes
R1384 T2109 T2106 pobj spermatocytes,in
R1385 T2110 T2102 punct .,was
R1386 T2112 T2113 nsubj TRIP13,appeared
R1387 T2114 T2115 aux to,be
R1388 T2115 T2113 xcomp be,appeared
R1389 T2116 T2117 amod nuclear,localized
R1390 T2117 T2115 acomp localized,be
R1391 T2118 T2113 punct .,appeared
R1392 T2120 T2121 expl There,was
R1393 T2122 T2123 det no,staining
R1394 T2123 T2121 attr staining,was
R1395 T2124 T2123 amod such,staining
R1396 T2125 T2123 prep of,staining
R1397 T2126 T2125 pobj nuclei,of
R1398 T2127 T2121 prep in,was
R1399 T2128 T2129 nmod mutant,tubules
R1400 T2129 T2127 pobj tubules,in
R1401 T2130 T2129 amod seminiferous,tubules
R1402 T2131 T2132 punct (,1F
R1403 T2132 T2121 parataxis 1F,was
R1404 T2133 T2132 compound Figure,1F
R1405 T2134 T2132 punct ),1F
R1406 T2135 T2121 punct .,was
R1407 T2137 T2138 aux To,assess
R1408 T2138 T2140 advcl assess,used
R1409 T2139 T2138 advmod further,assess
R1410 T2141 T2142 det the,localization
R1411 T2142 T2138 dobj localization,assess
R1412 T2143 T2142 amod nuclear,localization
R1413 T2144 T2140 punct ", ",used
R1414 T2145 T2140 nsubjpass TRIP13,used
R1415 T2146 T2140 auxpass was,used
R1416 T2147 T2148 aux to,probe
R1417 T2148 T2140 advcl probe,used
R1418 T2149 T2150 amod meiotic,chromosomes
R1419 T2150 T2148 dobj chromosomes,probe
R1420 T2151 T2150 acl prepared,chromosomes
R1421 T2152 T2151 prep by,prepared
R1422 T2153 T2154 compound surface,spreading
R1423 T2154 T2152 pobj spreading,by
R1424 T2155 T2154 prep of,spreading
R1425 T2156 T2157 compound spermatocyte,nuclei
R1426 T2157 T2155 pobj nuclei,of
R1427 T2158 T2140 punct .,used
R1428 T2160 T2161 prep In,was
R1429 T2162 T2163 amod wild,type
R1430 T2163 T2160 pobj type,In
R1431 T2164 T2161 punct ", ",was
R1432 T2165 T2161 expl there,was
R1433 T2166 T2167 amod diffuse,staining
R1434 T2167 T2161 attr staining,was
R1435 T2168 T2167 amod nuclear,staining
R1436 T2169 T2167 punct ", ",staining
R1437 T2170 T2167 cc and,staining
R1438 T2171 T2172 det no,evidence
R1439 T2172 T2167 conj evidence,staining
R1440 T2173 T2172 prep of,evidence
R1441 T2174 T2173 pobj concentration,of
R1442 T2175 T2174 prep on,concentration
R1443 T2176 T2177 compound SC,cores
R1444 T2177 T2175 pobj cores,on
R1445 T2178 T2174 punct (,concentration
R1446 T2179 T2174 acl marked,concentration
R1447 T2180 T2179 agent by,marked
R1448 T2181 T2182 det the,SYCP3
R1449 T2182 T2180 pobj SYCP3,by
R1450 T2183 T2184 amod axial,element
R1451 T2184 T2185 compound element,protein
R1452 T2185 T2182 compound protein,SYCP3
R1453 T2186 T2172 punct ),evidence
R1454 T2187 T2172 prep at,evidence
R1455 T2188 T2189 det any,substage
R1456 T2189 T2187 pobj substage,at
R1457 T2190 T2189 amod meiotic,substage
R1458 T2191 T2192 punct (,1G
R1459 T2192 T2161 parataxis 1G,was
R1460 T2193 T2192 compound Figure,1G
R1461 T2194 T2192 punct ),1G
R1462 T2195 T2161 punct .,was
R1463 T2197 T2198 compound TRIP13,signal
R1464 T2198 T2199 nsubj signal,was
R1465 T2200 T2201 advmod noticeably,absent
R1466 T2201 T2199 acomp absent,was
R1467 T2202 T2199 prep in,was
R1468 T2203 T2204 nmod mutant,nuclei
R1469 T2204 T2202 pobj nuclei,in
R1470 T2205 T2204 amod meiotic,nuclei
R1471 T2206 T2199 punct .,was
R1474 T2305 T2304 prep Due,Infertility
R1475 T2306 T2305 pcomp to,Due
R1476 T2307 T2308 amod Meiotic,Disruption
R1477 T2308 T2305 pobj Disruption,Due
R1478 T2309 T2304 prep in,Infertility
R1479 T2310 T2311 npadvmod TRIP13,Deficient
R1480 T2311 T2313 amod Deficient,Meiocytes
R1481 T2312 T2311 punct -,Deficient
R1482 T2313 T2309 pobj Meiocytes,in
R1483 T2315 T2316 nsubj Homozygotes,had
R1484 T2317 T2315 prep of,Homozygotes
R1485 T2318 T2319 det both,sexes
R1486 T2319 T2317 pobj sexes,of
R1487 T2320 T2321 amod small,gonads
R1488 T2321 T2316 dobj gonads,had
R1489 T2322 T2323 punct (,2C
R1490 T2323 T2316 parataxis 2C,had
R1491 T2324 T2323 compound Figure,2C
R1492 T2325 T2323 punct ;,2C
R1493 T2326 T2323 advcl see,2C
R1494 T2327 T2326 advmod below,see
R1495 T2328 T2323 punct ),2C
R1496 T2329 T2316 cc and,had
R1497 T2330 T2316 conj were,had
R1498 T2331 T2332 advmod invariably,sterile
R1499 T2332 T2330 acomp sterile,were
R1500 T2333 T2316 punct .,had
R1501 T2335 T2336 nsubj Ovaries,were
R1502 T2337 T2335 prep of,Ovaries
R1503 T2338 T2339 amod adult,females
R1504 T2339 T2337 pobj females,of
R1505 T2340 T2341 compound Trip13Gt,Gt
R1506 T2341 T2339 compound Gt,females
R1507 T2342 T2341 punct /,Gt
R1508 T2343 T2344 advmod severely,dysmorphic
R1509 T2344 T2336 acomp dysmorphic,were
R1510 T2345 T2336 cc and,were
R1511 T2346 T2336 conj had,were
R1512 T2347 T2348 amod few,follicles
R1513 T2348 T2346 dobj follicles,had
R1514 T2349 T2348 cc or,follicles
R1515 T2350 T2348 det no,follicles
R1516 T2351 T2352 punct (,3A
R1517 T2352 T2346 parataxis 3A,had
R1518 T2353 T2352 compound Figure,3A
R1519 T2354 T2352 cc and,3A
R1520 T2355 T2352 conj 3B,3A
R1521 T2356 T2352 punct ),3A
R1522 T2357 T2336 punct .,were
R1523 T2359 T2360 det The,majority
R1524 T2360 T2361 nsubj majority,occurred
R1525 T2362 T2360 prep of,majority
R1526 T2363 T2364 compound oocyte,loss
R1527 T2364 T2362 pobj loss,of
R1528 T2365 T2361 prep in,occurred
R1529 T2366 T2367 amod late,embryogenesis
R1530 T2367 T2365 pobj embryogenesis,in
R1531 T2368 T2365 cc or,in
R1532 T2369 T2370 advmod early,in
R1533 T2370 T2365 conj in,in
R1534 T2371 T2372 amod postnatal,development
R1535 T2372 T2370 pobj development,in
R1536 T2373 T2361 punct ", ",occurred
R1537 T2374 T2375 mark since,were
R1538 T2375 T2361 advcl were,occurred
R1539 T2376 T2377 nummod 2,d
R1540 T2377 T2378 nmod d,ovaries
R1541 T2378 T2375 nsubj ovaries,were
R1542 T2379 T2378 advmod postpartum,ovaries
R1543 T2380 T2381 advmod markedly,smaller
R1544 T2381 T2375 acomp smaller,were
R1545 T2382 T2381 prep than,smaller
R1546 T2383 T2382 pobj those,than
R1547 T2384 T2383 prep of,those
R1548 T2385 T2386 compound control,littermates
R1549 T2386 T2384 pobj littermates,of
R1550 T2387 T2375 punct ", ",were
R1551 T2388 T2375 cc and,were
R1552 T2389 T2390 aux were,lacking
R1553 T2390 T2375 conj lacking,were
R1554 T2391 T2390 dobj oocytes,lacking
R1555 T2392 T2391 cc or,oocytes
R1556 T2393 T2394 advmod newly,forming
R1557 T2394 T2395 amod forming,follicles
R1558 T2395 T2391 conj follicles,oocytes
R1559 T2396 T2397 punct (,3C
R1560 T2397 T2361 parataxis 3C,occurred
R1561 T2398 T2397 compound Figure,3C
R1562 T2399 T2397 cc and,3C
R1563 T2400 T2397 conj 3D,3C
R1564 T2401 T2397 punct ),3C
R1565 T2402 T2361 punct .,occurred
R1566 T2404 T2405 advmod Thus,failed
R1567 T2406 T2405 punct ", ",failed
R1568 T2407 T2405 nsubj oocytes,failed
R1569 T2408 T2409 aux to,progress
R1570 T2409 T2405 xcomp progress,failed
R1571 T2410 T2409 prep to,progress
R1572 T2411 T2412 det the,phase
R1573 T2412 T2410 pobj phase,to
R1574 T2413 T2412 nmod dictyate,phase
R1575 T2414 T2415 punct (,resting
R1576 T2415 T2413 parataxis resting,dictyate
R1577 T2416 T2415 punct ),resting
R1578 T2417 T2405 punct .,failed
R1579 T2419 T2420 mark Since,observed
R1580 T2420 T2422 advcl observed,indicates
R1581 T2421 T2420 nsubj we,observed
R1582 T2423 T2420 dobj oocytes,observed
R1583 T2424 T2423 prep with,oocytes
R1584 T2425 T2426 compound pachytene,stage
R1585 T2426 T2427 compound stage,chromosomes
R1586 T2427 T2424 pobj chromosomes,with
R1587 T2428 T2427 prep in,chromosomes
R1588 T2429 T2430 nummod 17.5,d
R1589 T2430 T2431 nmod d,ovaries
R1590 T2431 T2428 pobj ovaries,in
R1591 T2432 T2433 nmod Trip13Gt,Gt
R1592 T2433 T2431 nmod Gt,ovaries
R1593 T2434 T2433 punct /,Gt
R1594 T2435 T2431 amod embryonic,ovaries
R1595 T2436 T2437 punct (,data
R1596 T2437 T2420 meta data,observed
R1597 T2438 T2437 amod unpublished,data
R1598 T2439 T2437 punct ),data
R1599 T2440 T2422 punct ", ",indicates
R1600 T2441 T2422 nsubj this,indicates
R1601 T2442 T2443 mark that,eliminated
R1602 T2443 T2422 ccomp eliminated,indicates
R1603 T2444 T2443 nsubjpass oocytes,eliminated
R1604 T2445 T2443 auxpass were,eliminated
R1605 T2446 T2443 advmod somewhere,eliminated
R1606 T2447 T2446 prep between,somewhere
R1607 T2448 T2447 pobj pachynema,between
R1608 T2449 T2448 cc and,pachynema
R1609 T2450 T2448 conj dictyate,pachynema
R1610 T2451 T2422 punct .,indicates
R1611 T2453 T2454 amod Histological,sections
R1612 T2454 T2455 nsubj sections,revealed
R1613 T2456 T2454 prep of,sections
R1614 T2457 T2458 compound mutant,testes
R1615 T2458 T2456 pobj testes,of
R1616 T2459 T2460 det a,lack
R1617 T2460 T2455 dobj lack,revealed
R1618 T2461 T2460 prep of,lack
R1619 T2462 T2463 amod postmeiotic,types
R1620 T2463 T2461 pobj types,of
R1621 T2464 T2463 compound cell,types
R1622 T2465 T2466 dep that,are
R1623 T2466 T2463 relcl are,types
R1624 T2467 T2466 acomp characteristic,are
R1625 T2468 T2467 prep of,characteristic
R1626 T2469 T2470 amod wild,type
R1627 T2470 T2472 nmod type,tubules
R1628 T2471 T2470 punct -,type
R1629 T2472 T2468 pobj tubules,of
R1630 T2473 T2472 amod seminiferous,tubules
R1631 T2474 T2475 punct (,3E
R1632 T2475 T2455 parataxis 3E,revealed
R1633 T2476 T2475 compound Figure,3E
R1634 T2477 T2475 punct ),3E
R1635 T2478 T2455 punct .,revealed
R1636 T2480 T2481 det The,tubules
R1637 T2481 T2486 nsubj tubules,contained
R1638 T2482 T2483 advmod most,advanced
R1639 T2483 T2481 amod advanced,tubules
R1640 T2484 T2483 advmod developmentally,advanced
R1641 T2485 T2481 amod seminiferous,tubules
R1642 T2487 T2488 amod adluminal,spermatocytes
R1643 T2488 T2486 dobj spermatocytes,contained
R1644 T2489 T2488 prep with,spermatocytes
R1645 T2490 T2491 amod condensed,chromatin
R1646 T2491 T2489 pobj chromatin,with
R1647 T2492 T2488 amod characteristic,spermatocytes
R1648 T2493 T2492 prep of,characteristic
R1649 T2494 T2493 pobj pachynema,of
R1650 T2495 T2496 punct (,3F
R1651 T2496 T2486 parataxis 3F,contained
R1652 T2497 T2496 compound Figure,3F
R1653 T2498 T2496 punct ),3F
R1654 T2499 T2486 punct .,contained
R1655 T2501 T2502 det The,absence
R1656 T2502 T2503 nsubj absence,is
R1657 T2504 T2502 prep of,absence
R1658 T2505 T2506 amod coordinated,progression
R1659 T2506 T2504 pobj progression,of
R1660 T2507 T2506 amod spermatogenic,progression
R1661 T2508 T2502 prep beyond,absence
R1662 T2509 T2510 det this,stage
R1663 T2510 T2508 pobj stage,beyond
R1664 T2511 T2503 acomp indicative,is
R1665 T2512 T2511 prep of,indicative
R1666 T2513 T2514 det a,arrest
R1667 T2514 T2512 pobj arrest,of
R1668 T2515 T2514 compound pachytene,arrest
R1669 T2516 T2503 punct .,is
R1670 T2518 T2519 nsubjpass This,revealed
R1671 T2520 T2519 auxpass was,revealed
R1672 T2521 T2522 advmod more,clearly
R1673 T2522 T2519 advmod clearly,revealed
R1674 T2523 T2519 agent by,revealed
R1675 T2524 T2525 compound chromosome,analysis
R1676 T2525 T2523 pobj analysis,by
R1677 T2526 T2527 punct (,see
R1678 T2527 T2519 parataxis see,revealed
R1679 T2528 T2527 advmod below,see
R1680 T2529 T2527 punct ),see
R1681 T2530 T2519 punct .,revealed
R1682 T2532 T2533 det Some,sections
R1683 T2533 T2534 nsubj sections,contained
R1684 T2535 T2533 prep of,sections
R1685 T2536 T2537 amod adult,tubules
R1686 T2537 T2535 pobj tubules,of
R1687 T2538 T2537 amod seminiferous,tubules
R1688 T2539 T2540 amod postmeiotic,spermatids
R1689 T2540 T2534 dobj spermatids,contained
R1690 T2541 T2542 punct (,3G
R1691 T2542 T2534 parataxis 3G,contained
R1692 T2543 T2542 compound Figure,3G
R1693 T2544 T2542 punct ),3G
R1694 T2545 T2534 punct ", ",contained
R1695 T2546 T2547 mark although,saw
R1696 T2547 T2534 advcl saw,contained
R1697 T2548 T2547 nsubj we,saw
R1698 T2549 T2550 det no,sperm
R1699 T2550 T2547 dobj sperm,saw
R1700 T2551 T2550 amod motile,sperm
R1701 T2552 T2550 amod epididymal,sperm
R1702 T2553 T2534 punct .,contained
R1703 T2555 T2556 det These,defects
R1704 T2556 T2559 nsubj defects,stand
R1705 T2557 T2556 amod drastic,defects
R1706 T2558 T2556 amod meiotic,defects
R1707 T2560 T2559 prep in,stand
R1708 T2561 T2560 pobj contrast,in
R1709 T2562 T2561 prep to,contrast
R1710 T2563 T2562 pobj yeast,to
R1711 T2564 T2563 cc and,yeast
R1712 T2565 T2566 compound C.,elegans
R1713 T2566 T2563 conj elegans,yeast
R1714 T2567 T2563 punct ", ",yeast
R1715 T2568 T2569 prep in,has
R1716 T2569 T2563 relcl has,yeast
R1717 T2570 T2568 pobj which,in
R1718 T2571 T2569 nsubj deletion,has
R1719 T2572 T2571 prep of,deletion
R1720 T2573 T2572 pobj Pch2,of
R1721 T2574 T2573 advmod alone,Pch2
R1722 T2575 T2576 amod minor,effects
R1723 T2576 T2569 dobj effects,has
R1724 T2577 T2576 prep on,effects
R1725 T2578 T2579 compound spore,gamete
R1726 T2579 T2581 compound gamete,development
R1727 T2580 T2579 punct /,gamete
R1728 T2581 T2577 pobj development,on
R1729 T2582 T2583 punct [,8
R1730 T2583 T2569 parataxis 8,has
R1731 T2584 T2583 nummod 2,8
R1732 T2585 T2583 punct ",",8
R1733 T2586 T2583 punct ],8
R1734 T2587 T2559 punct .,stand
R1742 T2968 T2969 npadvmod TRIP13,Deficient
R1743 T2969 T2971 amod Deficient,Meiocytes
R1744 T2970 T2969 punct -,Deficient
R1745 T2971 T2972 nsubj Meiocytes,Undergo
R1746 T2973 T2974 amod Homologous,Synapsis
R1747 T2974 T2972 dobj Synapsis,Undergo
R1748 T2975 T2974 compound Chromosome,Synapsis
R1749 T2976 T2972 prep Despite,Undergo
R1750 T2977 T2978 det the,Presence
R1751 T2978 T2976 pobj Presence,Despite
R1752 T2979 T2978 prep of,Presence
R1753 T2980 T2981 amod Unrepaired,DSBs
R1754 T2981 T2979 pobj DSBs,of
R1755 T2982 T2978 prep in,Presence
R1756 T2983 T2982 pobj Pachynema,in
R1757 T2985 T2986 aux To,characterize
R1758 T2986 T2988 advcl characterize,immunostained
R1759 T2987 T2986 advmod better,characterize
R1760 T2989 T2990 det the,degree
R1761 T2990 T2986 dobj degree,characterize
R1762 T2991 T2990 prep of,degree
R1763 T2992 T2993 amod meiotic,progression
R1764 T2993 T2991 pobj progression,of
R1765 T2994 T2990 prep in,degree
R1766 T2995 T2996 compound Trip13Gt,Gt
R1767 T2996 T2998 compound Gt,spermatocytes
R1768 T2997 T2996 punct /,Gt
R1769 T2998 T2994 pobj spermatocytes,in
R1770 T2999 T2988 punct ", ",immunostained
R1771 T3000 T2988 nsubj we,immunostained
R1772 T3001 T3002 compound chromosome,spreads
R1773 T3002 T2988 dobj spreads,immunostained
R1774 T3003 T3002 prep for,spreads
R1775 T3004 T3003 pobj SYCP3,for
R1776 T3005 T3004 cc and,SYCP3
R1777 T3006 T3004 conj SYCP1,SYCP3
R1778 T3007 T3004 punct ", ",SYCP3
R1779 T3008 T3004 appos components,SYCP3
R1780 T3009 T3008 prep of,components
R1781 T3010 T3011 det the,elements
R1782 T3011 T3009 pobj elements,of
R1783 T3012 T3013 amod axial,lateral
R1784 T3013 T3011 amod lateral,elements
R1785 T3014 T3013 punct /,lateral
R1786 T3015 T3011 cc and,elements
R1787 T3016 T3017 amod transverse,filaments
R1788 T3017 T3011 conj filaments,elements
R1789 T3018 T3011 punct ", ",elements
R1790 T3019 T3011 advmod respectively,elements
R1791 T3020 T3011 punct ", ",elements
R1792 T3021 T3011 prep of,elements
R1793 T3022 T3023 det the,complex
R1794 T3023 T3021 pobj complex,of
R1795 T3024 T3023 amod synaptonemal,complex
R1796 T3025 T3023 punct (,complex
R1797 T3026 T3023 appos SC,complex
R1798 T3027 T2988 punct ),immunostained
R1799 T3028 T2988 punct .,immunostained
R1800 T3030 T3031 compound Pachytene,nuclei
R1801 T3031 T3033 nsubj nuclei,assemble
R1802 T3032 T3031 compound spermatocyte,nuclei
R1803 T3034 T3031 prep from,nuclei
R1804 T3035 T3036 amod postpubertal,testes
R1805 T3036 T3034 pobj testes,from
R1806 T3037 T3036 compound mutant,testes
R1807 T3038 T3033 aux could,assemble
R1808 T3039 T3040 amod normal,cores
R1809 T3040 T3033 dobj cores,assemble
R1810 T3041 T3040 compound SC,cores
R1811 T3042 T3033 cc and,assemble
R1812 T3043 T3033 conj exhibited,assemble
R1813 T3044 T3045 amod full,synapsis
R1814 T3045 T3043 dobj synapsis,exhibited
R1815 T3046 T3045 prep of,synapsis
R1816 T3047 T3046 pobj chromosomes,of
R1817 T3048 T3049 mark as,judged
R1818 T3049 T3043 advcl judged,exhibited
R1819 T3050 T3049 prep by,judged
R1820 T3051 T3050 pcomp colabeling,by
R1821 T3052 T3051 prep of,colabeling
R1822 T3053 T3052 pobj SYCP1,of
R1823 T3054 T3053 cc and,SYCP1
R1824 T3055 T3053 conj SYCP3,SYCP1
R1825 T3056 T3051 prep along,colabeling
R1826 T3057 T3058 det the,lengths
R1827 T3058 T3056 pobj lengths,along
R1828 T3059 T3058 amod full,lengths
R1829 T3060 T3058 prep of,lengths
R1830 T3061 T3062 det all,autosomes
R1831 T3062 T3060 pobj autosomes,of
R1832 T3063 T3064 punct (,4A
R1833 T3064 T3051 parataxis 4A,colabeling
R1834 T3065 T3064 compound Figure,4A
R1835 T3066 T3064 punct ),4A
R1836 T3067 T3033 punct .,assemble
R1837 T3069 T3070 advmod Additionally,synapsed
R1838 T3071 T3070 punct ", ",synapsed
R1839 T3072 T3073 det the,chromosomes
R1840 T3073 T3070 nsubjpass chromosomes,synapsed
R1841 T3074 T3073 nmod X,chromosomes
R1842 T3075 T3074 cc and,X
R1843 T3076 T3074 conj Y,X
R1844 T3077 T3070 auxpass were,synapsed
R1845 T3078 T3070 advmod normally,synapsed
R1846 T3079 T3070 prep at,synapsed
R1847 T3080 T3081 poss their,region
R1848 T3081 T3079 pobj region,at
R1849 T3082 T3081 amod pseudoautosomal,region
R1850 T3083 T3070 punct .,synapsed
R1851 T3085 T3086 advmod More,spermatocytes
R1852 T3086 T3094 nsubj spermatocytes,contained
R1853 T3087 T3086 amod prepubertal,spermatocytes
R1854 T3088 T3086 punct (,spermatocytes
R1855 T3089 T3090 nummod 17.5,d
R1856 T3090 T3091 nmod d,postpartum
R1857 T3091 T3086 nmod postpartum,spermatocytes
R1858 T3092 T3086 punct ),spermatocytes
R1859 T3093 T3086 compound mutant,spermatocytes
R1860 T3095 T3096 amod asynaptic,chromosomes
R1861 T3096 T3094 dobj chromosomes,contained
R1862 T3097 T3095 cc or,asynaptic
R1863 T3098 T3099 advmod terminally,asynapsed
R1864 T3099 T3095 conj asynapsed,asynaptic
R1865 T3100 T3094 prep than,contained
R1866 T3101 T3102 npadvmod age,matched
R1867 T3102 T3104 amod matched,controls
R1868 T3103 T3102 punct -,matched
R1869 T3104 T3100 pobj controls,than
R1870 T3105 T3106 punct (,4B
R1871 T3106 T3094 parataxis 4B,contained
R1872 T3107 T3108 nummod 62.5,%
R1873 T3108 T3106 dep %,4B
R1874 T3109 T3108 cc versus,%
R1875 T3110 T3111 nummod 25,%
R1876 T3111 T3108 conj %,%
R1877 T3112 T3108 punct ", ",%
R1878 T3113 T3108 advmod respectively,%
R1879 T3114 T3106 punct ;,4B
R1880 T3115 T3106 compound Figure,4B
R1881 T3116 T3106 punct ),4B
R1882 T3117 T3094 punct .,contained
R1883 T3119 T3120 nsubj We,attribute
R1884 T3121 T3120 dobj this,attribute
R1885 T3122 T3120 prep to,attribute
R1886 T3123 T3124 det a,delay
R1887 T3124 T3122 pobj delay,to
R1888 T3125 T3124 prep in,delay
R1889 T3126 T3127 det the,wave
R1890 T3127 T3125 pobj wave,in
R1891 T3128 T3127 amod first,wave
R1892 T3129 T3127 prep of,wave
R1893 T3130 T3131 amod postnatal,spermatogenesis
R1894 T3131 T3129 pobj spermatogenesis,of
R1895 T3132 T3133 punct (,2D
R1896 T3133 T3124 parataxis 2D,delay
R1897 T3134 T3133 compound Figure,2D
R1898 T3135 T3133 cc and,2D
R1899 T3136 T3133 conj 2E,2D
R1900 T3137 T3133 punct ),2D
R1901 T3138 T3124 punct ", ",delay
R1902 T3139 T3140 advmod likely,related
R1903 T3140 T3124 acl related,delay
R1904 T3141 T3140 prep to,related
R1905 T3142 T3143 amod systemic,retardation
R1906 T3143 T3141 pobj retardation,to
R1907 T3144 T3143 amod developmental,retardation
R1908 T3145 T3146 punct (,2A
R1909 T3146 T3140 parataxis 2A,related
R1910 T3147 T3146 compound Figure,2A
R1911 T3148 T3146 cc and,2A
R1912 T3149 T3146 conj 2B,2A
R1913 T3150 T3146 punct ),2A
R1914 T3151 T3120 punct .,attribute
R1915 T3153 T3154 advmod Nevertheless,suggested
R1916 T3155 T3154 punct ", ",suggested
R1917 T3156 T3157 mark since,progress
R1918 T3157 T3154 advcl progress,suggested
R1919 T3158 T3159 compound Trip13Gt,Gt
R1920 T3159 T3161 compound Gt,spermatocytes
R1921 T3160 T3159 punct /,Gt
R1922 T3161 T3157 nsubj spermatocytes,progress
R1923 T3162 T3157 prep to,progress
R1924 T3163 T3162 pobj pachynema,to
R1925 T3164 T3157 prep with,progress
R1926 T3165 T3166 det no,abnormalities
R1927 T3166 T3164 pobj abnormalities,with
R1928 T3167 T3166 amod gross,abnormalities
R1929 T3168 T3166 compound SC,abnormalities
R1930 T3169 T3157 punct ", ",progress
R1931 T3170 T3157 cc and,progress
R1932 T3171 T3172 nsubjpass oocytes,eliminated
R1933 T3172 T3157 conj eliminated,progress
R1934 T3173 T3172 auxpass were,eliminated
R1935 T3174 T3175 advmod soon,after
R1936 T3175 T3172 prep after,eliminated
R1937 T3176 T3175 pobj birth,after
R1938 T3177 T3178 punct (,characteristic
R1939 T3178 T3172 parataxis characteristic,eliminated
R1940 T3179 T3178 det a,characteristic
R1941 T3180 T3178 prep of,characteristic
R1942 T3181 T3182 compound DNA,repair
R1943 T3182 T3183 compound repair,mutants
R1944 T3183 T3180 pobj mutants,of
R1945 T3184 T3185 punct [,13
R1946 T3185 T3178 parataxis 13,characteristic
R1947 T3186 T3185 punct ],13
R1948 T3187 T3178 punct ),characteristic
R1949 T3188 T3154 punct ", ",suggested
R1950 T3189 T3154 nsubj this,suggested
R1951 T3190 T3191 mark that,are
R1952 T3191 T3154 ccomp are,suggested
R1953 T3192 T3193 amod unrepaired,DSBs
R1954 T3193 T3191 nsubj DSBs,are
R1955 T3194 T3191 acomp responsible,are
R1956 T3195 T3194 prep for,responsible
R1957 T3196 T3197 amod eventual,arrest
R1958 T3197 T3195 pobj arrest,for
R1959 T3198 T3197 amod meiotic,arrest
R1960 T3199 T3197 cc and,arrest
R1961 T3200 T3197 conj elimination,arrest
R1962 T3201 T3154 punct .,suggested
R1963 T3213 T3214 npadvmod d,at
R1964 T3219 T3217 advmod c,t
R1965 T3222 T3221 amod a,u
R1966 T3227 T3226 punct ,f
R1967 T3229 T3228 pobj e,m
R1968 T3232 T3231 pobj ti,o
R1969 T3252 T3253 amod c,hromosomes
R1970 T3255 T3256 det a,variety
R1971 T3256 T3254 pobj variety,with
R1972 T3257 T3256 prep of,variety
R1973 T3258 T3257 pobj markers,of
R1974 T3259 T3260 dep that,are
R1975 T3260 T3258 relcl are,markers
R1976 T3261 T3260 acomp diagnostic,are
R1977 T3262 T3261 prep of,diagnostic
R1978 T3263 T3262 pobj recombination,of
R1979 T3264 T3263 cc and,recombination
R1980 T3265 T3263 conj synapsis,recombination
R1981 T3268 T3269 nsubj Recombination,appeared
R1982 T3270 T3268 prep in,Recombination
R1983 T3271 T3272 compound Trip13Gt,Gt
R1984 T3272 T3274 compound Gt,spermatocytes
R1985 T3273 T3272 punct /,Gt
R1986 T3274 T3270 pobj spermatocytes,in
R1987 T3275 T3276 aux to,initiate
R1988 T3276 T3269 xcomp initiate,appeared
R1989 T3277 T3276 advmod normally,initiate
R1990 T3278 T3279 mark as,judged
R1991 T3279 T3276 advcl judged,initiate
R1992 T3280 T3279 prep by,judged
R1993 T3281 T3282 det the,presence
R1994 T3282 T3280 pobj presence,by
R1995 T3283 T3282 prep of,presence
R1996 T3284 T3283 pobj γH2AX,of
R1997 T3285 T3282 prep in,presence
R1998 T3286 T3285 pobj leptonema,in
R1999 T3287 T3288 punct (,S2A
R2000 T3288 T3282 parataxis S2A,presence
R2001 T3289 T3288 compound Figure,S2A
R2002 T3290 T3288 cc and,S2A
R2003 T3291 T3288 conj S2B,S2A
R2004 T3292 T3288 punct ),S2A
R2005 T3293 T3282 punct ", ",presence
R2006 T3294 T3295 dep which,reflects
R2007 T3295 T3282 relcl reflects,presence
R2008 T3296 T3297 det the,presence
R2009 T3297 T3295 dobj presence,reflects
R2010 T3298 T3297 prep of,presence
R2011 T3299 T3300 advmod meiotically,induced
R2012 T3300 T3301 amod induced,DSBs
R2013 T3301 T3298 pobj DSBs,of
R2014 T3302 T3303 punct [,18
R2015 T3303 T3269 parataxis 18,appeared
R2016 T3304 T3303 punct ],18
R2017 T3305 T3269 punct .,appeared
R2018 T3307 T3308 nsubj RAD51,was
R2019 T3309 T3307 cc and,RAD51
R2020 T3310 T3309 punct /,and
R2021 T3311 T3309 cc or,and
R2022 T3312 T3307 conj DMC1,RAD51
R2023 T3313 T3307 punct ", ",RAD51
R2024 T3314 T3307 appos components,RAD51
R2025 T3315 T3314 prep of,components
R2026 T3316 T3317 amod early,recombination
R2027 T3317 T3318 compound recombination,nodules
R2028 T3318 T3315 pobj nodules,of
R2029 T3319 T3318 punct (,nodules
R2030 T3320 T3318 appos ERNs,nodules
R2031 T3321 T3308 punct ),was
R2032 T3322 T3308 punct ", ",was
R2033 T3323 T3308 advmod also,was
R2034 T3324 T3308 acomp present,was
R2035 T3325 T3308 prep as,was
R2036 T3326 T3327 amod abundant,foci
R2037 T3327 T3325 pobj foci,as
R2038 T3328 T3308 prep in,was
R2039 T3329 T3330 compound Trip13Gt,Gt
R2040 T3330 T3332 compound Gt,spermatocytes
R2041 T3331 T3330 punct /,Gt
R2042 T3332 T3328 pobj spermatocytes,in
R2043 T3333 T3332 compound zygotene,spermatocytes
R2044 T3334 T3335 punct (,data
R2045 T3335 T3308 parataxis data,was
R2046 T3336 T3335 amod unpublished,data
R2047 T3337 T3335 punct ;,data
R2048 T3338 T3339 det the,antibody
R2049 T3339 T3341 nsubj antibody,cross-reacts
R2050 T3340 T3339 amod anti-RAD51,antibody
R2051 T3341 T3335 ccomp cross-reacts,data
R2052 T3342 T3341 prep with,cross-reacts
R2053 T3343 T3342 pobj DMC1,with
R2054 T3344 T3335 punct ),data
R2055 T3345 T3308 punct ", ",was
R2056 T3346 T3308 advcl indicating,was
R2057 T3347 T3348 mark that,initiated
R2058 T3348 T3346 ccomp initiated,indicating
R2059 T3349 T3350 amod recombinational,repair
R2060 T3350 T3348 nsubjpass repair,initiated
R2061 T3351 T3350 prep of,repair
R2062 T3352 T3351 pobj DSBs,of
R2063 T3353 T3348 auxpass is,initiated
R2064 T3354 T3308 punct .,was
R2065 T3356 T3357 det The,complex
R2066 T3357 T3359 nsubj complex,appeared
R2067 T3358 T3357 compound cohesin,complex
R2068 T3360 T3357 punct ", ",complex
R2069 T3361 T3362 dep which,is
R2070 T3362 T3357 relcl is,complex
R2071 T3363 T3362 acomp essential,is
R2072 T3364 T3363 prep for,essential
R2073 T3365 T3364 pobj completion,for
R2074 T3366 T3365 cc and,completion
R2075 T3367 T3366 punct /,and
R2076 T3368 T3366 cc or,and
R2077 T3369 T3365 conj maintenance,completion
R2078 T3370 T3365 prep of,completion
R2079 T3371 T3372 amod synaptic,associations
R2080 T3372 T3370 pobj associations,of
R2081 T3373 T3359 punct ", ",appeared
R2082 T3374 T3375 aux to,assemble
R2083 T3375 T3359 xcomp assemble,appeared
R2084 T3376 T3375 advmod normally,assemble
R2085 T3377 T3378 mark as,judged
R2086 T3378 T3375 advcl judged,assemble
R2087 T3379 T3378 prep by,judged
R2088 T3380 T3379 pcomp immunolabeling,by
R2089 T3381 T3380 prep for,immunolabeling
R2090 T3382 T3383 det the,STAG3
R2091 T3383 T3381 pobj STAG3,for
R2092 T3384 T3385 npadvmod meiosis,specific
R2093 T3385 T3383 amod specific,STAG3
R2094 T3386 T3385 punct -,specific
R2095 T3387 T3383 compound cohesins,STAG3
R2096 T3388 T3389 punct (,S2C
R2097 T3389 T3383 parataxis S2C,STAG3
R2098 T3390 T3389 compound Figure,S2C
R2099 T3391 T3389 cc and,S2C
R2100 T3392 T3389 conj S2D,S2C
R2101 T3393 T3389 punct ),S2C
R2102 T3394 T3383 cc and,STAG3
R2103 T3395 T3383 conj REC8,STAG3
R2104 T3396 T3397 punct (,data
R2105 T3397 T3395 meta data,REC8
R2106 T3398 T3397 amod unpublished,data
R2107 T3399 T3397 punct ),data
R2108 T3400 T3359 punct .,appeared
R2109 T3402 T3403 mark Because,localizes
R2110 T3403 T3406 advcl localizes,tested
R2111 T3404 T3405 compound yeast,PCH2
R2112 T3405 T3403 nsubj PCH2,localizes
R2113 T3407 T3403 prep to,localizes
R2114 T3408 T3407 pobj telomeres,to
R2115 T3409 T3403 prep in,localizes
R2116 T3410 T3411 det a,manner
R2117 T3411 T3409 pobj manner,in
R2118 T3412 T3413 npadvmod Sir3p,dependent
R2119 T3413 T3411 amod dependent,manner
R2120 T3414 T3413 punct -,dependent
R2121 T3415 T3406 punct ", ",tested
R2122 T3416 T3406 nsubj we,tested
R2123 T3417 T3406 prep for,tested
R2124 T3418 T3419 amod possible,defects
R2125 T3419 T3417 pobj defects,for
R2126 T3420 T3419 compound telomere,defects
R2127 T3421 T3406 prep by,tested
R2128 T3422 T3421 pcomp immunolabeling,by
R2129 T3423 T3422 prep for,immunolabeling
R2130 T3424 T3423 pobj TRF2,for
R2131 T3425 T3424 punct ", ",TRF2
R2132 T3426 T3427 det a,component
R2133 T3427 T3424 appos component,TRF2
R2134 T3428 T3427 prep of,component
R2135 T3429 T3430 det a,complex
R2136 T3430 T3428 pobj complex,of
R2137 T3431 T3430 compound protein,complex
R2138 T3432 T3433 dep that,plays
R2139 T3433 T3430 relcl plays,complex
R2140 T3434 T3435 det an,role
R2141 T3435 T3433 dobj role,plays
R2142 T3436 T3435 amod essential,role
R2143 T3437 T3433 prep in,plays
R2144 T3438 T3439 compound telomere,protection
R2145 T3439 T3437 pobj protection,in
R2146 T3440 T3441 punct [,19
R2147 T3441 T3406 parataxis 19,tested
R2148 T3442 T3441 punct ],19
R2149 T3443 T3406 punct .,tested
R2150 T3445 T3446 nsubjpass It,localized
R2151 T3447 T3446 auxpass was,localized
R2152 T3448 T3446 prep to,localized
R2153 T3449 T3448 pobj telomeres,to
R2154 T3450 T3449 prep of,telomeres
R2155 T3451 T3452 preconj both,synapsed
R2156 T3452 T3454 amod synapsed,chromosomes
R2157 T3453 T3452 advmod fully,synapsed
R2158 T3454 T3450 pobj chromosomes,of
R2159 T3455 T3452 cc and,synapsed
R2160 T3456 T3457 advmod telomerically,asynaptic
R2161 T3457 T3452 conj asynaptic,synapsed
R2162 T3458 T3454 compound mutant,chromosomes
R2163 T3459 T3460 punct (,S2E
R2164 T3460 T3446 parataxis S2E,localized
R2165 T3461 T3460 compound Figure,S2E
R2166 T3462 T3460 cc and,S2E
R2167 T3463 T3460 conj S2F,S2E
R2168 T3464 T3460 punct ),S2E
R2169 T3465 T3446 punct .,localized
R2170 T3467 T3468 nsubj Defects,became
R2171 T3469 T3467 prep in,Defects
R2172 T3470 T3471 compound DSB,repair
R2173 T3471 T3469 pobj repair,in
R2174 T3472 T3468 acomp apparent,became
R2175 T3473 T3468 prep in,became
R2176 T3474 T3473 pobj pachynema,in
R2177 T3475 T3468 prep upon,became
R2178 T3476 T3475 pobj probing,upon
R2179 T3477 T3476 prep of,probing
R2180 T3478 T3479 compound mutant,nuclei
R2181 T3479 T3477 pobj nuclei,of
R2182 T3480 T3479 compound spermatocyte,nuclei
R2183 T3481 T3476 prep with,probing
R2184 T3482 T3481 pobj antibodies,with
R2185 T3483 T3482 prep against,antibodies
R2186 T3484 T3483 pobj molecules,against
R2187 T3485 T3484 acl involved,molecules
R2188 T3486 T3485 prep in,involved
R2189 T3487 T3488 amod various,stages
R2190 T3488 T3486 pobj stages,in
R2191 T3489 T3488 prep of,stages
R2192 T3490 T3489 pobj recombination,of
R2193 T3491 T3468 punct .,became
R2194 T3493 T3494 prep In,persisted
R2195 T3495 T3496 punct >,99
R2196 T3496 T3497 nummod 99,%
R2197 T3497 T3493 pobj %,In
R2198 T3498 T3497 prep of,%
R2199 T3499 T3500 compound Trip13Gt,Gt
R2200 T3500 T3502 compound Gt,chromosome
R2201 T3501 T3500 punct /,Gt
R2202 T3502 T3503 compound chromosome,spreads
R2203 T3503 T3498 pobj spreads,of
R2204 T3504 T3494 punct ", ",persisted
R2205 T3505 T3506 compound BLM,helicase
R2206 T3506 T3494 nsubj helicase,persisted
R2207 T3507 T3508 punct (,4C
R2208 T3508 T3506 parataxis 4C,helicase
R2209 T3509 T3508 compound Figure,4C
R2210 T3510 T3508 cc and,4C
R2211 T3511 T3508 conj 4D,4C
R2212 T3512 T3508 punct ),4C
R2213 T3513 T3506 punct ", ",helicase
R2214 T3514 T3515 compound RAD51,DMC1
R2215 T3515 T3506 conj DMC1,helicase
R2216 T3516 T3515 punct /,DMC1
R2217 T3517 T3518 punct (,4E
R2218 T3518 T3515 parataxis 4E,DMC1
R2219 T3519 T3518 compound Figure,4E
R2220 T3520 T3518 cc and,4E
R2221 T3521 T3518 conj 4F,4E
R2222 T3522 T3518 punct ),4E
R2223 T3523 T3515 punct ", ",DMC1
R2224 T3524 T3515 conj γH2AX,DMC1
R2225 T3525 T3526 punct (,4G
R2226 T3526 T3524 parataxis 4G,γH2AX
R2227 T3527 T3526 compound Figure,4G
R2228 T3528 T3526 cc and,4G
R2229 T3529 T3526 conj 4H,4G
R2230 T3530 T3526 punct ),4G
R2231 T3531 T3524 punct ", ",γH2AX
R2232 T3532 T3524 cc and,γH2AX
R2233 T3533 T3524 conj TOPBP1,γH2AX
R2234 T3534 T3533 punct (,TOPBP1
R2235 T3535 T3536 compound Figure,4I
R2236 T3536 T3533 parataxis 4I,TOPBP1
R2237 T3537 T3536 cc and,4I
R2238 T3538 T3536 conj 4J,4I
R2239 T3539 T3533 punct ),TOPBP1
R2240 T3540 T3506 appos all,helicase
R2241 T3541 T3494 advmod abnormally,persisted
R2242 T3542 T3494 prep on,persisted
R2243 T3543 T3544 amod synapsed,chromosomes
R2244 T3544 T3542 pobj chromosomes,on
R2245 T3545 T3494 punct .,persisted
R2246 T3547 T3548 prep For,contained
R2247 T3549 T3550 compound RAD51,DMC1
R2248 T3550 T3547 pobj DMC1,For
R2249 T3551 T3550 punct /,DMC1
R2250 T3552 T3548 punct ", ",contained
R2251 T3553 T3554 compound mutant,pachytene
R2252 T3554 T3548 nsubj pachytene,contained
R2253 T3555 T3548 dep spermatocytes,contained
R2254 T3556 T3557 quantmod 138,6
R2255 T3557 T3559 nummod 6,foci
R2256 T3558 T3557 punct ±,6
R2257 T3559 T3548 dobj foci,contained
R2258 T3560 T3559 punct (,foci
R2259 T3561 T3559 prep compared,foci
R2260 T3562 T3561 prep to,compared
R2261 T3563 T3564 quantmod 11,3
R2262 T3564 T3566 nummod 3,foci
R2263 T3565 T3564 punct ±,3
R2264 T3566 T3562 pobj foci,to
R2265 T3567 T3566 prep in,foci
R2266 T3568 T3569 amod wild,type
R2267 T3569 T3567 pobj type,in
R2268 T3570 T3566 punct ", ",foci
R2269 T3571 T3572 dep most,were
R2270 T3572 T3566 relcl were,foci
R2271 T3573 T3571 prep of,most
R2272 T3574 T3573 pobj which,of
R2273 T3575 T3572 prep on,were
R2274 T3576 T3577 det the,body
R2275 T3577 T3575 pobj body,on
R2276 T3578 T3577 compound XY,body
R2277 T3579 T3559 punct ),foci
R2278 T3580 T3548 punct ", ",contained
R2279 T3581 T3548 advmod down,contained
R2280 T3582 T3581 prep from,down
R2281 T3583 T3584 nummod 218,13
R2282 T3584 T3582 pobj 13,from
R2283 T3585 T3584 punct ±,13
R2284 T3586 T3584 prep in,13
R2285 T3587 T3586 pobj zygonema,in
R2286 T3588 T3584 punct (,13
R2287 T3589 T3584 prep compared,13
R2288 T3590 T3589 prep to,compared
R2289 T3591 T3592 quantmod 220,13
R2290 T3592 T3594 nummod 13,foci
R2291 T3593 T3592 punct ±,13
R2292 T3594 T3590 pobj foci,to
R2293 T3595 T3594 prep in,foci
R2294 T3596 T3597 amod wild,type
R2295 T3597 T3595 pobj type,in
R2296 T3598 T3584 punct ),13
R2297 T3599 T3548 punct .,contained
R2298 T3601 T3602 nsubj TOPBP1,is
R2299 T3603 T3604 det a,protein
R2300 T3604 T3602 attr protein,is
R2301 T3605 T3606 compound DNA,damage
R2302 T3606 T3607 compound damage,checkpoint
R2303 T3607 T3604 compound checkpoint,protein
R2304 T3608 T3607 punct –,checkpoint
R2305 T3609 T3604 acl involved,protein
R2306 T3610 T3609 prep in,involved
R2307 T3611 T3612 compound ATM,protein
R2308 T3612 T3613 npadvmod protein,dependent
R2309 T3613 T3615 amod dependent,activation
R2310 T3614 T3613 punct –,dependent
R2311 T3615 T3610 pobj activation,in
R2312 T3616 T3615 prep of,activation
R2313 T3617 T3618 compound ATR,protein
R2314 T3618 T3616 pobj protein,of
R2315 T3619 T3620 punct [,21
R2316 T3620 T3602 parataxis 21,is
R2317 T3621 T3620 nummod 20,21
R2318 T3622 T3620 punct ",",21
R2319 T3623 T3620 punct ],21
R2320 T3624 T3602 punct .,is
R2321 T3626 T3627 nsubj It,binds
R2322 T3628 T3627 dobj sites,binds
R2323 T3629 T3628 prep of,sites
R2324 T3630 T3629 pobj DSBs,of
R2325 T3631 T3628 cc and,sites
R2326 T3632 T3633 amod unsynapsed,regions
R2327 T3633 T3628 conj regions,sites
R2328 T3634 T3633 prep of,regions
R2329 T3635 T3636 amod meiotic,chromosomes
R2330 T3636 T3634 pobj chromosomes,of
R2331 T3637 T3638 punct [,23
R2332 T3638 T3627 parataxis 23,binds
R2333 T3639 T3638 nummod 22,23
R2334 T3640 T3638 punct ",",23
R2335 T3641 T3638 punct ],23
R2336 T3642 T3627 punct .,binds
R2337 T3644 T3645 nsubjpass BLM,reported
R2338 T3646 T3645 aux has,reported
R2339 T3647 T3645 auxpass been,reported
R2340 T3648 T3649 aux to,colocalize
R2341 T3649 T3645 xcomp colocalize,reported
R2342 T3650 T3649 prep with,colocalize
R2343 T3651 T3652 nmod markers,RPA
R2344 T3652 T3650 pobj RPA,with
R2345 T3653 T3652 punct (,RPA
R2346 T3654 T3652 cc and,RPA
R2347 T3655 T3652 conj MSH4,RPA
R2348 T3656 T3652 punct ),RPA
R2349 T3657 T3652 prep of,RPA
R2350 T3658 T3657 pobj recombination,of
R2351 T3659 T3649 prep at,colocalize
R2352 T3660 T3659 pobj sites,at
R2353 T3661 T3660 amod distinct,sites
R2354 T3662 T3661 prep from,distinct
R2355 T3663 T3662 pobj those,from
R2356 T3664 T3665 dep that,become
R2357 T3665 T3663 relcl become,those
R2358 T3666 T3665 acomp resolved,become
R2359 T3667 T3665 prep as,become
R2360 T3668 T3667 pobj crossovers,as
R2361 T3669 T3668 punct (,crossovers
R2362 T3670 T3668 appos CO,crossovers
R2363 T3671 T3649 punct ),colocalize
R2364 T3672 T3673 punct [,24
R2365 T3673 T3649 parataxis 24,colocalize
R2366 T3674 T3673 punct ],24
R2367 T3675 T3645 punct .,reported
R2368 T3677 T3678 nsubj We,assessed
R2369 T3679 T3678 advmod therefore,assessed
R2370 T3680 T3681 det the,distribution
R2371 T3681 T3678 dobj distribution,assessed
R2372 T3682 T3681 prep of,distribution
R2373 T3683 T3682 pobj RPA,of
R2374 T3684 T3683 punct ", ",RPA
R2375 T3685 T3686 det the,protein
R2376 T3686 T3683 appos protein,RPA
R2377 T3687 T3686 compound ssDNA,protein
R2378 T3688 T3686 compound binding,protein
R2379 T3689 T3686 punct ", ",protein
R2380 T3690 T3691 dep which,is
R2381 T3691 T3686 relcl is,protein
R2382 T3692 T3691 advmod normally,is
R2383 T3693 T3691 acomp present,is
R2384 T3694 T3691 prep at,is
R2385 T3695 T3696 amod focal,sites
R2386 T3696 T3694 pobj sites,at
R2387 T3697 T3696 prep of,sites
R2388 T3698 T3699 amod synapsing,chromosomes
R2389 T3699 T3697 pobj chromosomes,of
R2390 T3700 T3699 amod meiotic,chromosomes
R2391 T3701 T3691 prep before,is
R2392 T3702 T3701 pcomp disappearing,before
R2393 T3703 T3702 prep in,disappearing
R2394 T3704 T3703 pobj mid-pachynema,in
R2395 T3705 T3706 punct [,25
R2396 T3706 T3678 parataxis 25,assessed
R2397 T3707 T3706 punct ],25
R2398 T3708 T3678 punct .,assessed
R2399 T3710 T3711 nsubjpass It,thought
R2400 T3712 T3711 auxpass is,thought
R2401 T3713 T3714 aux to,bind
R2402 T3714 T3711 xcomp bind,thought
R2403 T3715 T3716 compound D,loops
R2404 T3716 T3714 dobj loops,bind
R2405 T3717 T3716 punct -,loops
R2406 T3718 T3716 prep of,loops
R2407 T3719 T3720 compound recombination,intermediates
R2408 T3720 T3718 pobj intermediates,of
R2409 T3721 T3722 punct [,26
R2410 T3722 T3711 parataxis 26,thought
R2411 T3723 T3722 punct ],26
R2412 T3724 T3711 punct .,thought
R2413 T3726 T3727 nsubj RPA,persisted
R2414 T3728 T3727 advmod also,persisted
R2415 T3729 T3727 prep on,persisted
R2416 T3730 T3731 compound pachytene,chromosomes
R2417 T3731 T3729 pobj chromosomes,on
R2418 T3732 T3731 compound mutant,chromosomes
R2419 T3733 T3734 punct (,4K
R2420 T3734 T3727 parataxis 4K,persisted
R2421 T3735 T3734 compound Figure,4K
R2422 T3736 T3734 cc and,4K
R2423 T3737 T3734 conj 4L,4K
R2424 T3738 T3734 punct ),4K
R2425 T3739 T3727 punct .,persisted
R2426 T3741 T3742 det These,data
R2427 T3742 T3743 nsubj data,indicate
R2428 T3744 T3745 mark that,remain
R2429 T3745 T3743 ccomp remain,indicate
R2430 T3746 T3747 amod unrepaired,DSBs
R2431 T3747 T3745 nsubj DSBs,remain
R2432 T3748 T3747 punct ", ",DSBs
R2433 T3749 T3747 cc or,DSBs
R2434 T3750 T3751 amod unresolved,intermediates
R2435 T3751 T3747 conj intermediates,DSBs
R2436 T3752 T3751 compound recombination,intermediates
R2437 T3753 T3745 punct ", ",remain
R2438 T3754 T3745 prep in,remain
R2439 T3755 T3754 pobj pachynema,in
R2440 T3756 T3745 cc and,remain
R2441 T3757 T3745 conj activate,remain
R2442 T3758 T3759 det a,system
R2443 T3759 T3757 dobj system,activate
R2444 T3760 T3761 compound DNA,damage
R2445 T3761 T3762 compound damage,checkpoint
R2446 T3762 T3759 compound checkpoint,system
R2447 T3763 T3743 punct .,indicate
R2448 T3765 T3766 nsubjpass It,noted
R2449 T3767 T3766 aux should,noted
R2450 T3768 T3766 auxpass be,noted
R2451 T3769 T3770 mark that,stained
R2452 T3770 T3766 ccomp stained,noted
R2453 T3771 T3770 nsubjpass chromosomes,stained
R2454 T3772 T3771 acl affected,chromosomes
R2455 T3773 T3772 agent by,affected
R2456 T3774 T3775 amod meiotic,inactivation
R2457 T3775 T3773 pobj inactivation,by
R2458 T3776 T3777 compound sex,chromosome
R2459 T3777 T3775 compound chromosome,inactivation
R2460 T3778 T3775 punct (,inactivation
R2461 T3779 T3775 appos MSCI,inactivation
R2462 T3780 T3775 punct ),inactivation
R2463 T3781 T3775 cc and,inactivation
R2464 T3782 T3783 amod meiotic,silencing
R2465 T3783 T3775 conj silencing,inactivation
R2466 T3784 T3783 prep of,silencing
R2467 T3785 T3786 amod unpaired,chromatin
R2468 T3786 T3784 pobj chromatin,of
R2469 T3787 T3783 punct (,silencing
R2470 T3788 T3783 appos MSUC,silencing
R2471 T3789 T3770 punct ),stained
R2472 T3790 T3770 auxpass are,stained
R2473 T3791 T3770 advmod heavily,stained
R2474 T3792 T3770 agent by,stained
R2475 T3793 T3792 pobj antibodies,by
R2476 T3794 T3770 prep for,stained
R2477 T3795 T3796 amod several,molecules
R2478 T3796 T3794 pobj molecules,for
R2479 T3797 T3798 compound DSB,repair
R2480 T3798 T3799 npadvmod repair,associated
R2481 T3799 T3796 amod associated,molecules
R2482 T3800 T3799 punct -,associated
R2483 T3801 T3796 punct ", ",molecules
R2484 T3802 T3796 prep including,molecules
R2485 T3803 T3802 pobj γH2AX,including
R2486 T3804 T3766 punct .,noted
R2487 T3806 T3807 compound H2AX,phosphorylation
R2488 T3807 T3808 nsubjpass phosphorylation,conducted
R2489 T3809 T3807 prep due,phosphorylation
R2490 T3810 T3809 pcomp to,due
R2491 T3811 T3809 pobj MSCI,due
R2492 T3812 T3811 cc and,MSCI
R2493 T3813 T3811 conj MSUC,MSCI
R2494 T3814 T3808 auxpass is,conducted
R2495 T3815 T3808 prep by,conducted
R2496 T3816 T3815 pobj ATR,by
R2497 T3817 T3816 punct ", ",ATR
R2498 T3818 T3816 cc not,ATR
R2499 T3819 T3816 conj ATM,ATR
R2500 T3820 T3821 punct [,27
R2501 T3821 T3808 parataxis 27,conducted
R2502 T3822 T3823 punct –,29
R2503 T3823 T3821 prep 29,27
R2504 T3824 T3821 punct ],27
R2505 T3825 T3808 punct .,conducted
R2506 T3827 T3828 mark Since,synapsed
R2507 T3828 T3833 advcl synapsed,is
R2508 T3829 T3830 compound mutant,chromosomes
R2509 T3830 T3828 nsubjpass chromosomes,synapsed
R2510 T3831 T3828 auxpass are,synapsed
R2511 T3832 T3828 advmod fully,synapsed
R2512 T3834 T3828 punct ", ",synapsed
R2513 T3835 T3828 cc and,synapsed
R2514 T3836 T3837 nsubjpass MSUC,known
R2515 T3837 T3828 conj known,synapsed
R2516 T3838 T3837 auxpass is,known
R2517 T3839 T3840 aux to,occur
R2518 T3840 T3837 xcomp occur,known
R2519 T3841 T3842 advmod only,as
R2520 T3842 T3840 prep as,occur
R2521 T3843 T3844 det a,result
R2522 T3844 T3842 pobj result,as
R2523 T3845 T3844 prep of,result
R2524 T3846 T3845 pobj asynapsis,of
R2525 T3847 T3833 punct ", ",is
R2526 T3848 T3849 det the,decoration
R2527 T3849 T3833 nsubj decoration,is
R2528 T3850 T3849 prep of,decoration
R2529 T3851 T3852 compound Trip13Gt,Gt
R2530 T3852 T3854 compound Gt,chromosomes
R2531 T3853 T3852 punct /,Gt
R2532 T3854 T3850 pobj chromosomes,of
R2533 T3855 T3849 prep with,decoration
R2534 T3856 T3857 compound DNA,repair
R2535 T3857 T3858 compound repair,markers
R2536 T3858 T3855 pobj markers,with
R2537 T3859 T3860 advmod probably,attributable
R2538 T3860 T3833 acomp attributable,is
R2539 T3861 T3860 prep to,attributable
R2540 T3862 T3863 amod incomplete,repair
R2541 T3863 T3861 pobj repair,to
R2542 T3864 T3863 compound DNA,repair
R2543 T3865 T3866 advmod rather,than
R2544 T3866 T3863 cc than,repair
R2545 T3867 T3868 amod transcriptional,silencing
R2546 T3868 T3863 conj silencing,repair
R2547 T3869 T3833 punct .,is
R2548 T3871 T3872 advcl Consistent,observed
R2549 T3873 T3871 prep with,Consistent
R2550 T3874 T3875 det the,presence
R2551 T3875 T3873 pobj presence,with
R2552 T3876 T3875 prep of,presence
R2553 T3877 T3878 amod rare,spermatocytes
R2554 T3878 T3876 pobj spermatocytes,of
R2555 T3879 T3880 punct (,%
R2556 T3880 T3877 parataxis %,rare
R2557 T3881 T3882 punct <,1
R2558 T3882 T3880 nummod 1,%
R2559 T3883 T3880 punct ),%
R2560 T3884 T3885 compound Trip13Gt,Gt
R2561 T3885 T3878 compound Gt,spermatocytes
R2562 T3886 T3885 punct /,Gt
R2563 T3887 T3878 compound pachytene,spermatocytes
R2564 T3888 T3878 amod devoid,spermatocytes
R2565 T3889 T3888 prep of,devoid
R2566 T3890 T3891 amod persistent,markers
R2567 T3891 T3889 pobj markers,of
R2568 T3892 T3893 compound DNA,repair
R2569 T3893 T3891 compound repair,markers
R2570 T3894 T3878 punct ", ",spermatocytes
R2571 T3895 T3878 cc and,spermatocytes
R2572 T3896 T3897 compound testis,histology
R2573 T3897 T3878 conj histology,spermatocytes
R2574 T3898 T3897 acl showing,histology
R2575 T3899 T3900 det some,degree
R2576 T3900 T3898 dobj degree,showing
R2577 T3901 T3900 prep of,degree
R2578 T3902 T3903 amod postmeiotic,progression
R2579 T3903 T3901 pobj progression,of
R2580 T3904 T3905 punct (,3G
R2581 T3905 T3871 parataxis 3G,Consistent
R2582 T3906 T3905 compound Figure,3G
R2583 T3907 T3905 punct ),3G
R2584 T3908 T3872 punct ", ",observed
R2585 T3909 T3872 nsubj we,observed
R2586 T3910 T3911 preconj both,nuclei
R2587 T3911 T3872 dobj nuclei,observed
R2588 T3912 T3911 compound diplotene,nuclei
R2589 T3913 T3914 dep that,lacked
R2590 T3914 T3911 relcl lacked,nuclei
R2591 T3915 T3916 amod autosomal,DMC1
R2592 T3916 T3914 dobj DMC1,lacked
R2593 T3917 T3916 compound RAD51,DMC1
R2594 T3918 T3916 punct /,DMC1
R2595 T3919 T3916 cc and,DMC1
R2596 T3920 T3916 conj γH2AX,DMC1
R2597 T3921 T3922 punct (,S3D
R2598 T3922 T3914 parataxis S3D,lacked
R2599 T3923 T3922 nmod Figure,S3D
R2600 T3924 T3922 nmod S3A,S3D
R2601 T3925 T3922 punct –,S3D
R2602 T3926 T3922 punct ),S3D
R2603 T3927 T3911 punct ", ",nuclei
R2604 T3928 T3911 cc and,nuclei
R2605 T3929 T3928 advmod also,and
R2606 T3930 T3931 nmod metaphase,spreads
R2607 T3931 T3911 conj spreads,nuclei
R2608 T3932 T3930 nummod I,metaphase
R2609 T3933 T3931 prep with,spreads
R2610 T3934 T3935 nummod 20,bivalents
R2611 T3935 T3933 pobj bivalents,with
R2612 T3936 T3937 punct (,S3F
R2613 T3937 T3931 parataxis S3F,spreads
R2614 T3938 T3937 nmod Figure,S3F
R2615 T3939 T3937 nmod S3E,S3F
R2616 T3940 T3937 punct –,S3F
R2617 T3941 T3937 punct ),S3F
R2618 T3942 T3872 punct .,observed
R2619 T3944 T3945 mark Since,be
R2620 T3945 T3949 advcl be,arise
R2621 T3946 T3945 nsubj Trip13Gt,be
R2622 T3947 T3945 aux may,be
R2623 T3948 T3945 neg not,be
R2624 T3950 T3951 det a,null
R2625 T3951 T3945 attr null,be
R2626 T3952 T3951 amod complete,null
R2627 T3953 T3949 punct ", ",arise
R2628 T3954 T3955 det these,spermatocytes
R2629 T3955 T3949 nsubj spermatocytes,arise
R2630 T3956 T3955 nmod diplotene,spermatocytes
R2631 T3957 T3956 cc and,diplotene
R2632 T3958 T3956 conj metaphase,diplotene
R2633 T3959 T3958 nummod I,metaphase
R2634 T3960 T3949 aux might,arise
R2635 T3961 T3949 prep by,arise
R2636 T3962 T3961 pobj virtue,by
R2637 T3963 T3962 prep of,virtue
R2638 T3964 T3963 pcomp having,of
R2639 T3965 T3966 amod sufficient,TRIP13
R2640 T3966 T3964 dobj TRIP13,having
R2641 T3967 T3968 amod wild,type
R2642 T3968 T3966 compound type,TRIP13
R2643 T3969 T3968 punct -,type
R2644 T3970 T3949 punct .,arise
R2647 T4133 T4134 npadvmod CO,Associated
R2648 T4134 T4136 amod Associated,Markers
R2649 T4135 T4134 punct -,Associated
R2650 T4136 T4137 nsubj Markers,Appear
R2651 T4138 T4137 advmod Normally,Appear
R2652 T4139 T4137 prep in,Appear
R2653 T4140 T4141 det the,Absence
R2654 T4141 T4139 pobj Absence,in
R2655 T4142 T4141 prep of,Absence
R2656 T4143 T4142 pobj TRIP13,of
R2657 T4145 T4146 det The,persistence
R2658 T4146 T4147 nsubj persistence,suggests
R2659 T4148 T4146 prep of,persistence
R2660 T4149 T4148 pobj BLM,of
R2661 T4150 T4146 prep on,persistence
R2662 T4151 T4152 compound Trip13Gt,Gt
R2663 T4152 T4154 compound Gt,spermatocyte
R2664 T4153 T4152 punct /,Gt
R2665 T4154 T4155 compound spermatocyte,chromosomes
R2666 T4155 T4150 pobj chromosomes,on
R2667 T4156 T4157 mark that,correspond
R2668 T4157 T4147 ccomp correspond,suggests
R2669 T4158 T4159 advmod at,least
R2670 T4159 T4160 advmod least,subset
R2671 T4160 T4157 nsubj subset,correspond
R2672 T4161 T4160 det a,subset
R2673 T4162 T4160 prep of,subset
R2674 T4163 T4164 det the,DSBs
R2675 T4164 T4162 pobj DSBs,of
R2676 T4165 T4164 amod unrepaired,DSBs
R2677 T4166 T4157 prep to,correspond
R2678 T4167 T4166 pobj sites,to
R2679 T4168 T4167 prep of,sites
R2680 T4169 T4170 amod defective,repair
R2681 T4170 T4168 pobj repair,of
R2682 T4171 T4172 npadvmod NCO,recombinational
R2683 T4172 T4170 amod recombinational,repair
R2684 T4173 T4147 punct .,suggests
R2685 T4175 T4176 aux To,assess
R2686 T4176 T4177 advcl assess,examined
R2687 T4178 T4179 mark whether,occurs
R2688 T4179 T4176 ccomp occurs,assess
R2689 T4180 T4181 compound CO,recombination
R2690 T4181 T4179 nsubj recombination,occurs
R2691 T4182 T4179 prep in,occurs
R2692 T4183 T4184 det the,mutant
R2693 T4184 T4182 pobj mutant,in
R2694 T4185 T4177 punct ", ",examined
R2695 T4186 T4177 nsubj we,examined
R2696 T4187 T4188 det the,distribution
R2697 T4188 T4177 dobj distribution,examined
R2698 T4189 T4188 prep of,distribution
R2699 T4190 T4191 det the,proteins
R2700 T4191 T4189 pobj proteins,of
R2701 T4192 T4193 compound mismatch,repair
R2702 T4193 T4191 compound repair,proteins
R2703 T4194 T4191 appos MLH1,proteins
R2704 T4195 T4194 cc and,MLH1
R2705 T4196 T4194 conj MLH3,MLH1
R2706 T4197 T4191 punct ", ",proteins
R2707 T4198 T4199 dep which,are
R2708 T4199 T4191 relcl are,proteins
R2709 T4200 T4199 advmod normally,are
R2710 T4201 T4199 acomp detectable,are
R2711 T4202 T4199 prep as,are
R2712 T4203 T4202 pobj foci,as
R2713 T4204 T4203 prep in,foci
R2714 T4205 T4206 amod mid,late
R2715 T4206 T4208 amod late,pachynema
R2716 T4207 T4206 punct -,late
R2717 T4208 T4204 pobj pachynema,in
R2718 T4209 T4199 cc and,are
R2719 T4210 T4199 conj mark,are
R2720 T4211 T4212 det the,locations
R2721 T4212 T4210 dobj locations,mark
R2722 T4213 T4212 prep of,locations
R2723 T4214 T4213 pobj chiasmata,of
R2724 T4215 T4216 punct [,31
R2725 T4216 T4177 parataxis 31,examined
R2726 T4217 T4216 nummod 30,31
R2727 T4218 T4216 punct ",",31
R2728 T4219 T4216 punct ],31
R2729 T4220 T4177 punct .,examined
R2730 T4222 T4223 advmod Remarkably,formed
R2731 T4223 T4230 ccomp formed,observed
R2732 T4224 T4223 punct ", ",formed
R2733 T4225 T4226 nmod MLH1,foci
R2734 T4226 T4223 nsubjpass foci,formed
R2735 T4227 T4225 punct /,MLH1
R2736 T4228 T4225 nummod 3,MLH1
R2737 T4229 T4223 auxpass were,formed
R2738 T4231 T4230 punct ;,observed
R2739 T4232 T4230 nsubj we,observed
R2740 T4233 T4234 quantmod 1,2
R2741 T4234 T4236 nummod 2,foci
R2742 T4235 T4234 punct –,2
R2743 T4236 T4230 dobj foci,observed
R2744 T4237 T4238 punct /,chromosome
R2745 T4238 T4236 prep chromosome,foci
R2746 T4239 T4230 prep as,observed
R2747 T4240 T4239 prep in,as
R2748 T4241 T4242 amod wild,type
R2749 T4242 T4240 pobj type,in
R2750 T4243 T4239 cc and,as
R2751 T4244 T4239 conj at,as
R2752 T4245 T4246 amod typical,levels
R2753 T4246 T4244 pobj levels,at
R2754 T4247 T4246 amod overall,levels
R2755 T4248 T4249 punct (,10
R2756 T4249 T4246 parataxis 10,levels
R2757 T4250 T4251 nsubj MLH3,2
R2758 T4251 T4249 ccomp 2,10
R2759 T4252 T4251 punct =,2
R2760 T4253 T4251 quantmod 23,2
R2761 T4254 T4251 punct ±,2
R2762 T4255 T4249 punct ", ",10
R2763 T4256 T4249 nsubj N,10
R2764 T4257 T4249 punct =,10
R2765 T4258 T4249 punct ;,10
R2766 T4259 T4260 punct [,32
R2767 T4260 T4249 parataxis 32,10
R2768 T4261 T4260 nummod 30,32
R2769 T4262 T4260 punct ",",32
R2770 T4263 T4260 punct ],32
R2771 T4264 T4249 punct ),10
R2772 T4265 T4230 prep on,observed
R2773 T4266 T4267 amod mid,late
R2774 T4267 T4269 nmod late,pachytene
R2775 T4268 T4267 punct -,late
R2776 T4269 T4270 compound pachytene,chromosomes
R2777 T4270 T4265 pobj chromosomes,on
R2778 T4271 T4272 punct (,4M
R2779 T4272 T4230 parataxis 4M,observed
R2780 T4273 T4272 compound Figure,4M
R2781 T4274 T4272 cc and,4M
R2782 T4275 T4272 conj 4N,4M
R2783 T4276 T4272 punct ;,4M
R2784 T4277 T4278 nsubj MLH1,shown
R2785 T4278 T4272 ccomp shown,4M
R2786 T4279 T4278 neg not,shown
R2787 T4280 T4272 punct ),4M
R2788 T4281 T4230 punct .,observed
R2789 T4283 T4284 mark Since,had
R2790 T4284 T4294 advcl had,was
R2791 T4285 T4286 punct <,1
R2792 T4286 T4287 nummod 1,%
R2793 T4287 T4284 nsubj %,had
R2794 T4288 T4287 prep of,%
R2795 T4289 T4290 compound Trip13Gt,Gt
R2796 T4290 T4292 compound Gt,pachytene
R2797 T4291 T4290 punct /,Gt
R2798 T4292 T4293 compound pachytene,nuclei
R2799 T4293 T4288 pobj nuclei,of
R2800 T4295 T4296 amod normal,repair
R2801 T4296 T4284 dobj repair,had
R2802 T4297 T4298 punct (,see
R2803 T4298 T4284 parataxis see,had
R2804 T4299 T4300 mark as,judged
R2805 T4300 T4298 advcl judged,see
R2806 T4301 T4300 prep by,judged
R2807 T4302 T4301 pobj absence,by
R2808 T4303 T4302 prep of,absence
R2809 T4304 T4305 amod persistent,markers
R2810 T4305 T4303 pobj markers,of
R2811 T4306 T4307 compound DSB,repair
R2812 T4307 T4305 compound repair,markers
R2813 T4308 T4298 punct ;,see
R2814 T4309 T4298 dobj above,see
R2815 T4310 T4298 punct ),see
R2816 T4311 T4284 punct ", ",had
R2817 T4312 T4284 cc but,had
R2818 T4313 T4314 nsubj most,had
R2819 T4314 T4284 conj had,had
R2820 T4315 T4313 prep of,most
R2821 T4316 T4317 det the,nuclei
R2822 T4317 T4315 pobj nuclei,of
R2823 T4318 T4317 compound pachytene,nuclei
R2824 T4319 T4320 nmod MLH1,foci
R2825 T4320 T4314 dobj foci,had
R2826 T4321 T4319 punct /,MLH1
R2827 T4322 T4319 nummod 3,MLH1
R2828 T4323 T4294 punct ", ",was
R2829 T4324 T4294 nsubj it,was
R2830 T4325 T4294 acomp unlikely,was
R2831 T4326 T4327 mark that,formed
R2832 T4327 T4294 ccomp formed,was
R2833 T4328 T4329 det the,foci
R2834 T4329 T4327 nsubj foci,formed
R2835 T4330 T4329 nmod MLH1,foci
R2836 T4331 T4330 punct /,MLH1
R2837 T4332 T4330 nummod 3,MLH1
R2838 T4333 T4334 advmod only,on
R2839 T4334 T4327 prep on,formed
R2840 T4335 T4334 pobj chromosomes,on
R2841 T4336 T4335 prep with,chromosomes
R2842 T4337 T4338 advmod fully,repaired
R2843 T4338 T4339 amod repaired,DSBs
R2844 T4339 T4336 pobj DSBs,with
R2845 T4340 T4294 punct .,was
R2846 T4342 T4343 aux To,test
R2847 T4343 T4344 advcl test,conducted
R2848 T4345 T4343 dobj this,test
R2849 T4346 T4343 advmod directly,test
R2850 T4347 T4344 punct ", ",conducted
R2851 T4348 T4344 nsubj we,conducted
R2852 T4349 T4350 amod double,staining
R2853 T4350 T4344 dobj staining,conducted
R2854 T4351 T4344 prep for,conducted
R2855 T4352 T4351 pobj MLH1,for
R2856 T4353 T4352 cc and,MLH1
R2857 T4354 T4355 compound RAD51,DMC1
R2858 T4355 T4352 conj DMC1,MLH1
R2859 T4356 T4355 punct /,DMC1
R2860 T4357 T4344 punct .,conducted
R2861 T4359 T4360 compound MLH1,foci
R2862 T4360 T4361 nsubj foci,were
R2863 T4362 T4361 acomp present,were
R2864 T4363 T4361 prep on,were
R2865 T4364 T4363 pobj chromosomes,on
R2866 T4365 T4366 dep that,contained
R2867 T4366 T4364 relcl contained,chromosomes
R2868 T4367 T4366 advmod also,contained
R2869 T4368 T4369 amod numerous,foci
R2870 T4369 T4366 dobj foci,contained
R2871 T4370 T4371 compound RAD51,DMC1
R2872 T4371 T4369 compound DMC1,foci
R2873 T4372 T4371 punct /,DMC1
R2874 T4373 T4374 punct (,4O
R2875 T4374 T4366 parataxis 4O,contained
R2876 T4375 T4374 compound Figure,4O
R2877 T4376 T4374 cc and,4O
R2878 T4377 T4374 conj 4P,4O
R2879 T4378 T4374 punct ),4O
R2880 T4379 T4361 punct .,were
R2881 T4381 T4382 aux To,assess
R2882 T4382 T4383 advcl assess,treated
R2883 T4384 T4385 mark whether,represent
R2884 T4385 T4382 ccomp represent,assess
R2885 T4386 T4387 det these,foci
R2886 T4387 T4385 nsubj foci,represent
R2887 T4388 T4387 nmod MLH1,foci
R2888 T4389 T4388 punct /,MLH1
R2889 T4390 T4388 nummod 3,MLH1
R2890 T4391 T4387 prep in,foci
R2891 T4392 T4393 compound Trip13Gt,Gt
R2892 T4393 T4395 compound Gt,pachytene
R2893 T4394 T4393 punct /,Gt
R2894 T4395 T4396 compound pachytene,spermatocytes
R2895 T4396 T4391 pobj spermatocytes,in
R2896 T4397 T4398 compound CO,events
R2897 T4398 T4385 dobj events,represent
R2898 T4399 T4398 acl completed,events
R2899 T4400 T4399 prep to,completed
R2900 T4401 T4402 det a,point
R2901 T4402 T4400 pobj point,to
R2902 T4403 T4404 advmod where,maintain
R2903 T4404 T4402 relcl maintain,point
R2904 T4405 T4404 nsubj they,maintain
R2905 T4406 T4404 aux could,maintain
R2906 T4407 T4408 amod interhomolog,attachments
R2907 T4408 T4404 dobj attachments,maintain
R2908 T4409 T4404 prep though,maintain
R2909 T4410 T4411 det the,end
R2910 T4411 T4409 pobj end,though
R2911 T4412 T4411 prep of,end
R2912 T4413 T4412 pobj prophase,of
R2913 T4414 T4413 nummod I,prophase
R2914 T4415 T4383 punct ", ",treated
R2915 T4416 T4383 nsubj we,treated
R2916 T4417 T4418 amod testicular,cells
R2917 T4418 T4383 dobj cells,treated
R2918 T4419 T4418 prep from,cells
R2919 T4420 T4421 quantmod 17.5,20.5
R2920 T4421 T4423 nummod 20.5,d
R2921 T4422 T4421 punct –,20.5
R2922 T4423 T4425 npadvmod d,old
R2923 T4424 T4423 punct -,d
R2924 T4425 T4427 amod old,mice
R2925 T4426 T4425 punct -,old
R2926 T4427 T4419 pobj mice,from
R2927 T4428 T4427 nmod control,mice
R2928 T4429 T4428 punct (,control
R2929 T4430 T4431 punct +,+
R2930 T4431 T4428 appos +,control
R2931 T4432 T4431 punct /,+
R2932 T4433 T4428 punct ),control
R2933 T4434 T4428 punct ", ",control
R2934 T4435 T4436 compound Trip13Gt,Gt
R2935 T4436 T4428 conj Gt,control
R2936 T4437 T4436 punct /,Gt
R2937 T4438 T4436 punct ", ",Gt
R2938 T4439 T4436 cc and,Gt
R2939 T4440 T4436 conj Dmc1,Gt
R2940 T4441 T4440 punct −,Dmc1
R2941 T4442 T4440 punct /,Dmc1
R2942 T4443 T4440 punct −,Dmc1
R2943 T4444 T4383 prep with,treated
R2944 T4445 T4446 det the,inhibitor
R2945 T4446 T4444 pobj inhibitor,with
R2946 T4447 T4448 compound protein,phosphatase
R2947 T4448 T4446 compound phosphatase,inhibitor
R2948 T4449 T4450 amod okadaic,acid
R2949 T4450 T4446 appos acid,inhibitor
R2950 T4451 T4446 punct (,inhibitor
R2951 T4452 T4446 appos OA,inhibitor
R2952 T4453 T4446 punct ),inhibitor
R2953 T4454 T4446 punct ", ",inhibitor
R2954 T4455 T4456 det a,chemical
R2955 T4456 T4446 appos chemical,inhibitor
R2956 T4457 T4458 dep that,induces
R2957 T4458 T4456 relcl induces,chemical
R2958 T4459 T4458 dobj degradation,induces
R2959 T4460 T4459 prep of,degradation
R2960 T4461 T4462 det the,SC
R2961 T4462 T4460 pobj SC,of
R2962 T4463 T4459 punct ", ",degradation
R2963 T4464 T4465 compound chromosome,condensation
R2964 T4465 T4459 conj condensation,degradation
R2965 T4466 T4465 punct ", ",condensation
R2966 T4467 T4465 cc and,condensation
R2967 T4468 T4469 amod premature,progression
R2968 T4469 T4465 conj progression,condensation
R2969 T4470 T4469 prep to,progression
R2970 T4471 T4470 pobj metaphase,to
R2971 T4472 T4471 nummod I,metaphase
R2972 T4473 T4474 punct [,33
R2973 T4474 T4383 parataxis 33,treated
R2974 T4475 T4474 punct ],33
R2975 T4476 T4383 punct .,treated
R2976 T4478 T4479 nummod Fifteen,spreads
R2977 T4479 T4481 nsubjpass spreads,identified
R2978 T4480 T4479 compound metaphase,spreads
R2979 T4482 T4481 auxpass were,identified
R2980 T4483 T4481 prep for,identified
R2981 T4484 T4485 det each,genotype
R2982 T4485 T4483 pobj genotype,for
R2983 T4486 T4481 punct .,identified
R2984 T4488 T4489 mark Whereas,had
R2985 T4489 T4498 advcl had,had
R2986 T4490 T4489 nsubj all,had
R2987 T4491 T4490 prep of,all
R2988 T4492 T4493 det the,spreads
R2989 T4493 T4491 pobj spreads,of
R2990 T4494 T4493 nmod Dmc1,spreads
R2991 T4495 T4494 punct −,Dmc1
R2992 T4496 T4494 punct /,Dmc1
R2993 T4497 T4494 punct −,Dmc1
R2994 T4499 T4500 punct ∼,35
R2995 T4500 T4501 nummod 35,chromosomes
R2996 T4501 T4489 dobj chromosomes,had
R2997 T4502 T4500 cc or,35
R2998 T4503 T4500 conj more,35
R2999 T4504 T4501 amod condensed,chromosomes
R3000 T4505 T4498 punct ", ",had
R3001 T4506 T4498 nsubj all,had
R3002 T4507 T4506 prep of,all
R3003 T4508 T4509 det the,spreads
R3004 T4509 T4507 pobj spreads,of
R3005 T4510 T4511 punct +,+
R3006 T4511 T4509 punct +,spreads
R3007 T4512 T4511 punct /,+
R3008 T4513 T4511 cc and,+
R3009 T4514 T4515 compound Trip13Gt,Gt
R3010 T4515 T4511 conj Gt,+
R3011 T4516 T4515 punct /,Gt
R3012 T4517 T4518 quantmod 20,25
R3013 T4518 T4498 dobj 25,had
R3014 T4519 T4518 punct –,25
R3015 T4520 T4498 punct ", ",had
R3016 T4521 T4498 advcl suggesting,had
R3017 T4522 T4523 mark that,represent
R3018 T4523 T4521 ccomp represent,suggesting
R3019 T4524 T4525 det the,foci
R3020 T4525 T4523 nsubj foci,represent
R3021 T4526 T4525 nmod MLH1,foci
R3022 T4527 T4526 punct /,MLH1
R3023 T4528 T4526 nummod 3,MLH1
R3024 T4529 T4525 prep in,foci
R3025 T4530 T4531 compound Trip13Gt,Gt
R3026 T4531 T4533 compound Gt,pachytene
R3027 T4532 T4531 punct /,Gt
R3028 T4533 T4534 compound pachytene,spermatocytes
R3029 T4534 T4529 pobj spermatocytes,in
R3030 T4535 T4523 dobj sites,represent
R3031 T4536 T4535 prep of,sites
R3032 T4537 T4538 amod completed,COs
R3033 T4538 T4536 pobj COs,of
R3034 T4539 T4537 punct ", ",completed
R3035 T4540 T4537 cc or,completed
R3036 T4541 T4542 advmod near,completed
R3037 T4542 T4537 conj completed,completed
R3038 T4543 T4542 punct -,completed
R3039 T4544 T4538 punct ", ",COs
R3040 T4545 T4498 punct .,had
R3041 T4547 T4548 mark Because,made
R3042 T4548 T4552 advcl made,is
R3043 T4549 T4550 det the,preparations
R3044 T4550 T4548 nsubjpass preparations,made
R3045 T4551 T4548 auxpass were,made
R3046 T4553 T4548 prep from,made
R3047 T4554 T4555 amod whole,testes
R3048 T4555 T4553 pobj testes,from
R3049 T4556 T4552 punct ", ",is
R3050 T4557 T4552 nsubj it,is
R3051 T4558 T4552 acomp possible,is
R3052 T4559 T4560 mark that,were
R3053 T4560 T4552 ccomp were,is
R3054 T4561 T4562 det the,metaphases
R3055 T4562 T4560 nsubj metaphases,were
R3056 T4563 T4564 npadvmod univalent,containing
R3057 T4564 T4562 amod containing,metaphases
R3058 T4565 T4564 punct -,containing
R3059 T4566 T4562 prep from,metaphases
R3060 T4567 T4568 nmod Dmc1,mice
R3061 T4568 T4566 pobj mice,from
R3062 T4569 T4567 punct −,Dmc1
R3063 T4570 T4567 punct /,Dmc1
R3064 T4571 T4567 punct −,Dmc1
R3065 T4572 T4560 prep from,were
R3066 T4573 T4572 pobj spermatogonia,from
R3067 T4574 T4573 punct ", ",spermatogonia
R3068 T4575 T4573 cc not,spermatogonia
R3069 T4576 T4573 conj spermatocytes,spermatogonia
R3070 T4577 T4552 punct .,is
R3071 T4919 T4920 compound TRIP13,Deficiency
R3072 T4920 T4921 nsubj Deficiency,Alleviate
R3073 T4922 T4921 aux Does,Alleviate
R3074 T4923 T4921 neg Not,Alleviate
R3075 T4924 T4925 amod Meiotic,Arrest
R3076 T4925 T4926 compound Arrest,Phenotypes
R3077 T4926 T4921 dobj Phenotypes,Alleviate
R3078 T4927 T4926 prep of,Phenotypes
R3079 T4928 T4927 pobj Mutants,of
R3080 T4929 T4928 amod Defective,Mutants
R3081 T4930 T4929 prep in,Defective
R3082 T4931 T4930 pobj Synapsis,in
R3083 T4933 T4934 aux To,determine
R3084 T4934 T4935 advcl determine,analyzed
R3085 T4936 T4937 mark if,prevents
R3086 T4937 T4934 ccomp prevents,determine
R3087 T4938 T4939 compound TRIP13,deficiency
R3088 T4939 T4937 nsubj deficiency,prevents
R3089 T4940 T4937 dobj apoptosis,prevents
R3090 T4941 T4940 acl triggered,apoptosis
R3091 T4942 T4941 agent by,triggered
R3092 T4943 T4942 pobj asynapsis,by
R3093 T4944 T4941 prep as,triggered
R3094 T4945 T4944 prep in,as
R3095 T4946 T4947 compound C.,elegans
R3096 T4947 T4945 pobj elegans,in
R3097 T4948 T4935 punct ", ",analyzed
R3098 T4949 T4935 nsubj we,analyzed
R3099 T4950 T4935 dobj mice,analyzed
R3100 T4951 T4952 dep that,were
R3101 T4952 T4950 relcl were,mice
R3102 T4953 T4954 advmod doubly,mutant
R3103 T4954 T4952 acomp mutant,were
R3104 T4955 T4954 prep for,mutant
R3105 T4956 T4955 pobj Spo11,for
R3106 T4957 T4956 cc and,Spo11
R3107 T4958 T4956 conj Trip13,Spo11
R3108 T4959 T4935 punct .,analyzed
R3109 T4961 T4962 nsubj SPO11,is
R3110 T4963 T4964 det a,transesterase
R3111 T4964 T4962 attr transesterase,is
R3112 T4965 T4966 dep that,is
R3113 T4966 T4964 relcl is,transesterase
R3114 T4967 T4966 acomp essential,is
R3115 T4968 T4967 prep for,essential
R3116 T4969 T4970 det the,creation
R3117 T4970 T4968 pobj creation,for
R3118 T4971 T4970 prep of,creation
R3119 T4972 T4973 advmod genetically,programmed
R3120 T4973 T4974 amod programmed,DSB
R3121 T4974 T4971 pobj DSB,of
R3122 T4975 T4970 prep during,creation
R3123 T4976 T4975 pobj leptonema,during
R3124 T4977 T4976 prep of,leptonema
R3125 T4978 T4979 amod many,organisms
R3126 T4979 T4977 pobj organisms,of
R3127 T4980 T4979 punct ", ",organisms
R3128 T4981 T4979 prep including,organisms
R3129 T4982 T4981 pobj mice,including
R3130 T4983 T4984 punct [,18
R3131 T4984 T4966 parataxis 18,is
R3132 T4985 T4984 punct ],18
R3133 T4986 T4962 punct .,is
R3134 T4988 T4989 prep In,have
R3135 T4990 T4991 compound C.,elegans
R3136 T4991 T4988 pobj elegans,In
R3137 T4992 T4989 punct ", ",have
R3138 T4993 T4994 nmod spo,gametes
R3139 T4994 T4989 nsubj gametes,have
R3140 T4995 T4993 punct -,spo
R3141 T4996 T4993 nummod 11,spo
R3142 T4997 T4994 compound mutant,gametes
R3143 T4998 T4999 amod extensive,asynapsis
R3144 T4999 T4989 dobj asynapsis,have
R3145 T5000 T4999 punct ", ",asynapsis
R3146 T5001 T5002 dep which,triggers
R3147 T5002 T4999 relcl triggers,asynapsis
R3148 T5003 T5004 npadvmod PCH,dependent
R3149 T5004 T5007 amod dependent,apoptosis
R3150 T5005 T5003 punct -,PCH
R3151 T5006 T5003 nummod 2,PCH
R3152 T5007 T5002 dobj apoptosis,triggers
R3153 T5008 T5007 prep in,apoptosis
R3154 T5009 T5008 pobj pachynema,in
R3155 T5010 T5011 punct [,2
R3156 T5011 T4989 parataxis 2,have
R3157 T5012 T5011 punct ],2
R3158 T5013 T4989 punct .,have
R3159 T5015 T5016 prep In,are
R3160 T5017 T5015 pobj mice,In
R3161 T5018 T5016 punct ", ",are
R3162 T5019 T5020 nmod Spo11,spermatocytes
R3163 T5020 T5016 nsubj spermatocytes,are
R3164 T5021 T5019 punct −,Spo11
R3165 T5022 T5019 punct /,Spo11
R3166 T5023 T5019 punct −,Spo11
R3167 T5024 T5025 advmod severely,defective
R3168 T5025 T5016 acomp defective,are
R3169 T5026 T5016 prep in,are
R3170 T5027 T5028 amod homologous,synapsis
R3171 T5028 T5026 pobj synapsis,in
R3172 T5029 T5028 compound chromosome,synapsis
R3173 T5030 T5031 punct [,35
R3174 T5031 T5016 parataxis 35,are
R3175 T5032 T5031 nummod 34,35
R3176 T5033 T5031 punct ",",35
R3177 T5034 T5031 punct ],35
R3178 T5035 T5016 punct ", ",are
R3179 T5036 T5016 cc and,are
R3180 T5037 T5016 conj arrest,are
R3181 T5038 T5037 prep with,arrest
R3182 T5039 T5038 pobj chromosomes,with
R3183 T5040 T5037 prep in,arrest
R3184 T5041 T5042 det a,state
R3185 T5042 T5040 pobj state,in
R3186 T5043 T5042 amod characteristic,state
R3187 T5044 T5043 prep of,characteristic
R3188 T5045 T5046 det the,transition
R3189 T5046 T5044 pobj transition,of
R3190 T5047 T5048 compound zygotene,pachytene
R3191 T5048 T5046 compound pachytene,transition
R3192 T5049 T5048 punct /,pachytene
R3193 T5050 T5051 punct (,3H
R3194 T5051 T5037 parataxis 3H,arrest
R3195 T5052 T5051 compound Figure,3H
R3196 T5053 T5051 punct ),3H
R3197 T5054 T5016 punct .,are
R3198 T5056 T5057 nsubj Spermatocytes,progressed
R3199 T5058 T5056 prep in,Spermatocytes
R3200 T5059 T5060 nmod Trip13Gt,Gt
R3201 T5060 T5062 nmod Gt,testes
R3202 T5061 T5060 punct /,Gt
R3203 T5062 T5058 pobj testes,in
R3204 T5063 T5062 nmod Spo11,testes
R3205 T5064 T5063 punct −,Spo11
R3206 T5065 T5063 punct /,Spo11
R3207 T5066 T5063 punct −,Spo11
R3208 T5067 T5057 advmod maximally,progressed
R3209 T5068 T5057 prep to,progressed
R3210 T5069 T5070 det that,point
R3211 T5070 T5068 pobj point,to
R3212 T5071 T5057 prep before,progressed
R3213 T5072 T5071 pcomp undergoing,before
R3214 T5073 T5072 dobj death,undergoing
R3215 T5074 T5075 punct (,3I
R3216 T5075 T5072 parataxis 3I,undergoing
R3217 T5076 T5075 compound Figs,3I
R3218 T5077 T5075 punct ),3I
R3219 T5078 T5072 punct ", ",undergoing
R3220 T5079 T5080 advmod well,before
R3221 T5080 T5072 prep before,undergoing
R3222 T5081 T5082 det the,checkpoint
R3223 T5082 T5080 pobj checkpoint,before
R3224 T5083 T5082 compound spindle,checkpoint
R3225 T5084 T5085 dep that,eliminates
R3226 T5085 T5082 relcl eliminates,checkpoint
R3227 T5086 T5087 compound achiasmate,spermatocytes
R3228 T5087 T5085 dobj spermatocytes,eliminates
R3229 T5088 T5089 punct [,36
R3230 T5089 T5057 parataxis 36,progressed
R3231 T5090 T5089 punct ],36
R3232 T5091 T5057 punct .,progressed
R3233 T5093 T5094 expl There,was
R3234 T5095 T5096 det no,evidence
R3235 T5096 T5094 attr evidence,was
R3236 T5097 T5096 prep of,evidence
R3237 T5098 T5099 nmod metaphase,spermatocytes
R3238 T5099 T5097 pobj spermatocytes,of
R3239 T5100 T5098 nummod I,metaphase
R3240 T5101 T5099 cc or,spermatocytes
R3241 T5102 T5103 amod postmeiotic,spermatids
R3242 T5103 T5099 conj spermatids,spermatocytes
R3243 T5104 T5096 prep in,evidence
R3244 T5105 T5106 det these,testes
R3245 T5106 T5104 pobj testes,in
R3246 T5107 T5094 punct ", ",was
R3247 T5108 T5094 prep unlike,was
R3248 T5109 T5108 pobj those,unlike
R3249 T5110 T5109 acl seen,those
R3250 T5111 T5110 prep in,seen
R3251 T5112 T5113 nmod Trip13,mutants
R3252 T5113 T5111 pobj mutants,in
R3253 T5114 T5113 amod single,mutants
R3254 T5115 T5116 punct (,3G
R3255 T5116 T5094 parataxis 3G,was
R3256 T5117 T5116 compound Figure,3G
R3257 T5118 T5116 punct ),3G
R3258 T5119 T5094 punct .,was
R3259 T5121 T5122 prep In,disrupted
R3260 T5123 T5121 pobj contrast,In
R3261 T5124 T5123 prep to,contrast
R3262 T5125 T5126 det the,synapsis
R3263 T5126 T5124 pobj synapsis,to
R3264 T5127 T5126 amod complete,synapsis
R3265 T5128 T5126 prep in,synapsis
R3266 T5129 T5130 compound Trip13Gt,Gt
R3267 T5130 T5132 compound Gt,pachytene
R3268 T5131 T5130 punct /,Gt
R3269 T5132 T5133 compound pachytene,spermatocytes
R3270 T5133 T5128 pobj spermatocytes,in
R3271 T5134 T5135 punct (,5A
R3272 T5135 T5126 parataxis 5A,synapsis
R3273 T5136 T5135 compound Figure,5A
R3274 T5137 T5135 punct ),5A
R3275 T5138 T5126 punct ", ",synapsis
R3276 T5139 T5140 prep in,is
R3277 T5140 T5126 relcl is,synapsis
R3278 T5141 T5139 pobj which,in
R3279 T5142 T5140 nsubj SPO11,is
R3280 T5143 T5140 acomp available,is
R3281 T5144 T5140 prep in,is
R3282 T5145 T5144 pobj leptonema,in
R3283 T5146 T5147 aux to,initiate
R3284 T5147 T5140 advcl initiate,is
R3285 T5148 T5149 punct (,S2A
R3286 T5149 T5147 parataxis S2A,initiate
R3287 T5150 T5149 prep via,S2A
R3288 T5151 T5152 compound DSB,induction
R3289 T5152 T5150 pobj induction,via
R3290 T5153 T5149 punct ", ",S2A
R3291 T5154 T5149 compound Figure,S2A
R3292 T5155 T5149 cc and,S2A
R3293 T5156 T5149 conj S2B,S2A
R3294 T5157 T5149 punct ),S2A
R3295 T5158 T5159 det a,search
R3296 T5159 T5147 dobj search,initiate
R3297 T5160 T5161 npadvmod recombination,driven
R3298 T5161 T5159 amod driven,search
R3299 T5162 T5161 punct -,driven
R3300 T5163 T5159 compound homolog,search
R3301 T5164 T5122 punct ", ",disrupted
R3302 T5165 T5166 compound chromosome,synapsis
R3303 T5166 T5122 nsubjpass synapsis,disrupted
R3304 T5167 T5166 prep in,synapsis
R3305 T5168 T5169 advmod doubly,mutant
R3306 T5169 T5170 amod mutant,spermatocytes
R3307 T5170 T5167 pobj spermatocytes,in
R3308 T5171 T5122 auxpass was,disrupted
R3309 T5172 T5122 advmod highly,disrupted
R3310 T5173 T5122 prep as,disrupted
R3311 T5174 T5173 prep in,as
R3312 T5175 T5176 nmod Spo11,mutants
R3313 T5176 T5174 pobj mutants,in
R3314 T5177 T5176 amod single,mutants
R3315 T5178 T5179 punct (,5B
R3316 T5179 T5122 parataxis 5B,disrupted
R3317 T5180 T5179 compound Figure,5B
R3318 T5181 T5179 cc and,5B
R3319 T5182 T5179 conj 5C,5B
R3320 T5183 T5179 punct ),5B
R3321 T5184 T5122 punct .,disrupted
R3322 T5186 T5187 amod Identical,studies
R3323 T5187 T5188 nsubjpass studies,performed
R3324 T5189 T5188 auxpass were,performed
R3325 T5190 T5188 prep with,performed
R3326 T5191 T5190 pobj mice,with
R3327 T5192 T5191 amod deficient,mice
R3328 T5193 T5192 prep for,deficient
R3329 T5194 T5193 pobj Mei1,for
R3330 T5195 T5194 punct ", ",Mei1
R3331 T5196 T5197 det a,gene
R3332 T5197 T5194 appos gene,Mei1
R3333 T5198 T5199 npadvmod vertebrate,specific
R3334 T5199 T5197 amod specific,gene
R3335 T5200 T5199 punct -,specific
R3336 T5201 T5202 advmod also,required
R3337 T5202 T5197 acl required,gene
R3338 T5203 T5202 prep for,required
R3339 T5204 T5205 compound DSB,formation
R3340 T5205 T5203 pobj formation,for
R3341 T5206 T5205 cc and,formation
R3342 T5207 T5208 compound chromosome,synapsis
R3343 T5208 T5205 conj synapsis,formation
R3344 T5209 T5210 punct [,37
R3345 T5210 T5188 parataxis 37,performed
R3346 T5211 T5210 punct ],37
R3347 T5212 T5188 punct ", ",performed
R3348 T5213 T5188 prep with,performed
R3349 T5214 T5215 amod similar,results
R3350 T5215 T5213 pobj results,with
R3351 T5216 T5217 punct (,3J
R3352 T5217 T5188 parataxis 3J,performed
R3353 T5218 T5217 compound Figure,3J
R3354 T5219 T5217 cc and,3J
R3355 T5220 T5217 conj 3K,3J
R3356 T5221 T5217 punct ;,3J
R3357 T5222 T5223 nsubj immunocytology,shown
R3358 T5223 T5217 ccomp shown,3J
R3359 T5224 T5223 neg not,shown
R3360 T5225 T5217 punct ),3J
R3361 T5226 T5188 punct .,performed
R3362 T5229 T5230 amod In,y
R3363 T5232 T5233 compound e,a
R3364 T5233 T5234 compound a,s
R3365 T5238 T5239 amod d,e
R3366 T5241 T5242 amod le,tion
R3367 T5247 T5246 pobj PCH2,f
R3368 T5248 T5249 det the,arrest
R3369 T5249 T5245 dobj arrest,alleviates
R3370 T5250 T5249 compound pachytene,arrest
R3371 T5251 T5249 acl caused,arrest
R3372 T5252 T5251 agent by,caused
R3373 T5253 T5254 amod asynaptic,zip1
R3374 T5254 T5252 pobj zip1,by
R3375 T5255 T5254 compound mutants,zip1
R3376 T5256 T5254 cc and,zip1
R3377 T5257 T5254 conj zip2,zip1
R3378 T5258 T5259 punct [,8
R3379 T5259 T5245 parataxis 8,alleviates
R3380 T5260 T5259 punct ],8
R3381 T5261 T5245 punct .,alleviates
R3382 T5263 T5264 mark Although,be
R3383 T5264 T5268 advcl be,be
R3384 T5265 T5266 compound mouse,SYCP1
R3385 T5266 T5264 nsubj SYCP1,be
R3386 T5267 T5264 aux might,be
R3387 T5269 T5270 det a,equivalent
R3388 T5270 T5264 attr equivalent,be
R3389 T5271 T5270 amod functional,equivalent
R3390 T5272 T5270 prep of,equivalent
R3391 T5273 T5272 pobj Zip1p,of
R3392 T5274 T5268 punct ", ",be
R3393 T5275 T5276 mark because,arrest
R3394 T5276 T5268 advcl arrest,be
R3395 T5277 T5278 compound Sycp1,spermatocytes
R3396 T5278 T5276 nsubj spermatocytes,arrest
R3397 T5279 T5278 compound mutant,spermatocytes
R3398 T5280 T5276 prep at,arrest
R3399 T5281 T5282 advmod approximately,point
R3400 T5282 T5280 pobj point,at
R3401 T5283 T5282 det the,point
R3402 T5284 T5282 amod same,point
R3403 T5285 T5282 prep as,point
R3404 T5286 T5287 compound Trip13,mutants
R3405 T5287 T5285 pobj mutants,as
R3406 T5288 T5268 punct ", ",be
R3407 T5289 T5268 expl there,be
R3408 T5290 T5268 aux would,be
R3409 T5291 T5292 det no,opportunity
R3410 T5292 T5268 attr opportunity,be
R3411 T5293 T5294 aux to,observe
R3412 T5294 T5292 acl observe,opportunity
R3413 T5295 T5294 dobj bypass,observe
R3414 T5296 T5295 prep of,bypass
R3415 T5297 T5296 pobj Sycp1,of
R3416 T5298 T5297 punct −,Sycp1
R3417 T5299 T5297 punct /,Sycp1
R3418 T5300 T5297 punct −,Sycp1
R3419 T5301 T5268 punct .,be
R3420 T5303 T5304 mark Since,is
R3421 T5304 T5306 advcl is,suggested
R3422 T5305 T5304 nsubj Zip2p,is
R3423 T5307 T5304 acomp present,is
R3424 T5308 T5304 prep at,is
R3425 T5309 T5308 pobj sites,at
R3426 T5310 T5309 prep of,sites
R3427 T5311 T5312 amod axial,associations
R3428 T5312 T5310 pobj associations,of
R3429 T5313 T5308 punct ", ",at
R3430 T5314 T5315 advmod even,in
R3431 T5315 T5308 prep in,at
R3432 T5316 T5317 compound zip1,mutants
R3433 T5317 T5315 pobj mutants,in
R3434 T5318 T5306 punct ", ",suggested
R3435 T5319 T5306 nsubjpass it,suggested
R3436 T5320 T5306 aux has,suggested
R3437 T5321 T5306 auxpass been,suggested
R3438 T5322 T5323 mark that,promotes
R3439 T5323 T5306 ccomp promotes,suggested
R3440 T5324 T5323 nsubj Zip2p,promotes
R3441 T5325 T5323 dobj initiation,promotes
R3442 T5326 T5325 prep of,initiation
R3443 T5327 T5328 compound chromosome,synapsis
R3444 T5328 T5326 pobj synapsis,of
R3445 T5329 T5330 punct [,38
R3446 T5330 T5306 parataxis 38,suggested
R3447 T5331 T5330 punct ],38
R3448 T5332 T5306 punct .,suggested
R3449 T5334 T5335 det These,observations
R3450 T5335 T5336 nsubj observations,raise
R3451 T5337 T5338 det the,possibility
R3452 T5338 T5336 dobj possibility,raise
R3453 T5339 T5340 mark that,responds
R3454 T5340 T5338 acl responds,possibility
R3455 T5341 T5340 prep in,responds
R3456 T5342 T5341 pobj yeast,in
R3457 T5343 T5340 punct ", ",responds
R3458 T5344 T5340 nsubj Pch2p,responds
R3459 T5345 T5340 prep to,responds
R3460 T5346 T5347 compound synapsis,polymerization
R3461 T5347 T5345 pobj polymerization,to
R3462 T5348 T5349 advmod rather,than
R3463 T5349 T5347 cc than,polymerization
R3464 T5350 T5347 conj initiation,polymerization
R3465 T5351 T5336 punct .,raise
R3466 T5353 T5354 aux To,test
R3467 T5354 T5355 advcl test,performed
R3468 T5356 T5354 dobj this,test
R3469 T5357 T5355 punct ", ",performed
R3470 T5358 T5355 nsubj we,performed
R3471 T5359 T5360 compound epistasis,analysis
R3472 T5360 T5355 dobj analysis,performed
R3473 T5361 T5355 prep with,performed
R3474 T5362 T5363 det a,allele
R3475 T5363 T5361 pobj allele,with
R3476 T5364 T5363 compound Rec8,allele
R3477 T5365 T5363 punct (,allele
R3478 T5366 T5363 appos Rec8Mei8,allele
R3479 T5367 T5366 punct ", ",Rec8Mei8
R3480 T5368 T5366 acl abbreviated,Rec8Mei8
R3481 T5369 T5368 prep as,abbreviated
R3482 T5370 T5369 pobj Rec8,as
R3483 T5371 T5370 punct −,Rec8
R3484 T5372 T5355 punct ),performed
R3485 T5373 T5355 punct .,performed
R3486 T5375 T5376 amod Meiotic,chromosomes
R3487 T5376 T5377 nsubj chromosomes,undergo
R3488 T5378 T5376 prep of,chromosomes
R3489 T5379 T5380 compound Rec8,spermatocytes
R3490 T5380 T5378 pobj spermatocytes,of
R3491 T5381 T5380 compound mutant,spermatocytes
R3492 T5382 T5383 amod apparent,pairing
R3493 T5383 T5377 dobj pairing,undergo
R3494 T5384 T5383 compound homolog,pairing
R3495 T5385 T5383 cc and,pairing
R3496 T5386 T5387 amod interhomolog,initiation
R3497 T5387 T5383 conj initiation,pairing
R3498 T5388 T5387 amod synaptic,initiation
R3499 T5389 T5377 punct ", ",undergo
R3500 T5390 T5377 cc but,undergo
R3501 T5391 T5377 conj are,undergo
R3502 T5392 T5391 acomp defective,are
R3503 T5393 T5392 prep in,defective
R3504 T5394 T5395 compound DSB,repair
R3505 T5395 T5393 pobj repair,in
R3506 T5396 T5391 cc and,are
R3507 T5397 T5391 conj fail,are
R3508 T5398 T5399 aux to,maintain
R3509 T5399 T5397 xcomp maintain,fail
R3510 T5400 T5399 amod interhomolog,maintain
R3511 T5401 T5399 dep synapsis,maintain
R3512 T5402 T5403 punct [,40
R3513 T5403 T5391 parataxis 40,are
R3514 T5404 T5403 nummod 39,40
R3515 T5405 T5403 punct ",",40
R3516 T5406 T5403 punct ],40
R3517 T5407 T5377 punct .,undergo
R3518 T5409 T5410 advmod Rather,appear
R3519 T5411 T5410 punct ", ",appear
R3520 T5412 T5413 compound sister,chromatids
R3521 T5413 T5410 nsubj chromatids,appear
R3522 T5414 T5415 aux to,synapse
R3523 T5415 T5410 xcomp synapse,appear
R3524 T5416 T5410 cc and,appear
R3525 T5417 T5418 auxpass are,bound
R3526 T5418 T5410 conj bound,appear
R3527 T5419 T5418 prep by,bound
R3528 T5420 T5419 pobj SYCP1,by
R3529 T5421 T5418 prep along,bound
R3530 T5422 T5423 poss their,axes
R3531 T5423 T5421 pobj axes,along
R3532 T5424 T5410 punct .,appear
R3533 T5426 T5427 compound Rec8,mutants
R3534 T5427 T5428 nsubj mutants,progress
R3535 T5429 T5428 aux do,progress
R3536 T5430 T5428 neg not,progress
R3537 T5431 T5428 prep to,progress
R3538 T5432 T5431 pobj diplonema,to
R3539 T5433 T5432 cc or,diplonema
R3540 T5434 T5432 conj metaphase,diplonema
R3541 T5435 T5434 nummod I,metaphase
R3542 T5436 T5428 punct .,progress
R3543 T5438 T5439 amod Double,analysis
R3544 T5439 T5441 nsubj analysis,indicated
R3545 T5440 T5439 compound mutant,analysis
R3546 T5442 T5443 mark that,is
R3547 T5443 T5441 ccomp is,indicated
R3548 T5444 T5443 nsubj Rec8,is
R3549 T5445 T5443 acomp epistatic,is
R3550 T5446 T5445 prep to,epistatic
R3551 T5447 T5446 pobj Trip13,to
R3552 T5448 T5441 punct .,indicated
R3553 T5450 T5451 prep As,resembled
R3554 T5452 T5450 prep in,As
R3555 T5453 T5454 det the,experiments
R3556 T5454 T5452 pobj experiments,in
R3557 T5455 T5454 nmod Spo11,experiments
R3558 T5456 T5455 cc and,Spo11
R3559 T5457 T5455 conj Mei1,Spo11
R3560 T5458 T5451 punct ", ",resembled
R3561 T5459 T5451 nsubj histology,resembled
R3562 T5460 T5459 prep of,histology
R3563 T5461 T5460 pobj testes,of
R3564 T5462 T5461 amod deficient,testes
R3565 T5463 T5462 prep for,deficient
R3566 T5464 T5465 preconj both,REC8
R3567 T5465 T5463 pobj REC8,for
R3568 T5466 T5465 cc and,REC8
R3569 T5467 T5465 conj TRIP13,REC8
R3570 T5468 T5469 det the,mutant
R3571 T5469 T5451 dobj mutant,resembled
R3572 T5470 T5469 compound Rec8,mutant
R3573 T5471 T5451 punct ", ",resembled
R3574 T5472 T5451 prep with,resembled
R3575 T5473 T5474 det no,evidence
R3576 T5474 T5472 pobj evidence,with
R3577 T5475 T5474 prep of,evidence
R3578 T5476 T5475 pobj progression,of
R3579 T5477 T5476 prep to,progression
R3580 T5478 T5477 pobj metaphase,to
R3581 T5479 T5478 nummod I,metaphase
R3582 T5480 T5481 dep that,occurs
R3583 T5481 T5476 relcl occurs,progression
R3584 T5482 T5481 prep in,occurs
R3585 T5483 T5484 compound Trip13Gt,Gt
R3586 T5484 T5486 compound Gt,mice
R3587 T5485 T5484 punct /,Gt
R3588 T5486 T5482 pobj mice,in
R3589 T5487 T5488 punct (,3L
R3590 T5488 T5451 parataxis 3L,resembled
R3591 T5489 T5488 compound Figure,3L
R3592 T5490 T5488 cc and,3L
R3593 T5491 T5488 conj 3M,3L
R3594 T5492 T5488 punct ),3L
R3595 T5493 T5451 punct .,resembled
R3596 T5495 T5496 amod Immunocytological,analysis
R3597 T5496 T5497 nsubj analysis,showed
R3598 T5498 T5496 prep of,analysis
R3599 T5499 T5500 compound spread,chromosomes
R3600 T5500 T5498 pobj chromosomes,of
R3601 T5501 T5502 det a,failure
R3602 T5502 T5497 dobj failure,showed
R3603 T5503 T5502 prep of,failure
R3604 T5504 T5505 amod homologous,synapsis
R3605 T5505 T5503 pobj synapsis,of
R3606 T5506 T5505 compound chromosome,synapsis
R3607 T5507 T5502 prep in,failure
R3608 T5508 T5509 preconj both,spermatocytes
R3609 T5509 T5507 pobj spermatocytes,in
R3610 T5510 T5509 det the,spermatocytes
R3611 T5511 T5509 nmod Rec8,spermatocytes
R3612 T5512 T5511 punct −,Rec8
R3613 T5513 T5511 punct /,Rec8
R3614 T5514 T5511 punct −,Rec8
R3615 T5515 T5511 cc and,Rec8
R3616 T5516 T5517 nmod Rec8,Gt
R3617 T5517 T5511 conj Gt,Rec8
R3618 T5518 T5516 punct −,Rec8
R3619 T5519 T5516 punct /,Rec8
R3620 T5520 T5516 punct −,Rec8
R3621 T5521 T5517 compound Trip13Gt,Gt
R3622 T5522 T5517 punct /,Gt
R3623 T5523 T5497 punct ", ",showed
R3624 T5524 T5525 mark as,reported
R3625 T5525 T5497 advcl reported,showed
R3626 T5526 T5525 advmod previously,reported
R3627 T5527 T5525 prep for,reported
R3628 T5528 T5529 compound Rec8,mutants
R3629 T5529 T5527 pobj mutants,for
R3630 T5530 T5531 punct (,5D
R3631 T5531 T5497 parataxis 5D,showed
R3632 T5532 T5531 compound Figure,5D
R3633 T5533 T5531 cc and,5D
R3634 T5534 T5531 conj 5E,5D
R3635 T5535 T5531 punct ),5D
R3636 T5536 T5537 punct [,40
R3637 T5537 T5497 parataxis 40,showed
R3638 T5538 T5537 nummod 39,40
R3639 T5539 T5537 punct ",",40
R3640 T5540 T5537 punct ],40
R3641 T5541 T5497 punct .,showed
R3642 T5543 T5544 mark Although,indicate
R3643 T5544 T5547 advcl indicate,reported
R3644 T5545 T5546 amod subsequent,reports
R3645 T5546 T5544 nsubj reports,indicate
R3646 T5548 T5544 advmod otherwise,indicate
R3647 T5549 T5550 punct [,12
R3648 T5550 T5544 parataxis 12,indicate
R3649 T5551 T5550 nummod 10,12
R3650 T5552 T5550 punct ",",12
R3651 T5553 T5550 punct ],12
R3652 T5554 T5547 punct ", ",reported
R3653 T5555 T5547 nsubjpass deletion,reported
R3654 T5556 T5555 prep of,deletion
R3655 T5557 T5556 pobj PCH2,of
R3656 T5558 T5555 prep in,deletion
R3657 T5559 T5558 pobj yeast,in
R3658 T5560 T5547 auxpass was,reported
R3659 T5561 T5547 advmod originally,reported
R3660 T5562 T5563 aux to,alleviate
R3661 T5563 T5547 xcomp alleviate,reported
R3662 T5564 T5565 amod meiotic,arrest
R3663 T5565 T5563 dobj arrest,alleviate
R3664 T5566 T5565 acl caused,arrest
R3665 T5567 T5566 agent by,caused
R3666 T5568 T5567 pobj deficiency,by
R3667 T5569 T5568 prep for,deficiency
R3668 T5570 T5571 det the,homolog
R3669 T5571 T5569 pobj homolog,for
R3670 T5572 T5573 npadvmod meiosis,specific
R3671 T5573 T5571 amod specific,homolog
R3672 T5574 T5573 punct -,specific
R3673 T5575 T5571 compound RecA,homolog
R3674 T5576 T5571 appos DMC1,homolog
R3675 T5577 T5578 punct [,8
R3676 T5578 T5547 parataxis 8,reported
R3677 T5579 T5578 punct ],8
R3678 T5580 T5547 punct .,reported
R3679 T5582 T5583 aux To,investigate
R3680 T5583 T5584 advcl investigate,constructed
R3681 T5585 T5586 det this,relationship
R3682 T5586 T5583 dobj relationship,investigate
R3683 T5587 T5583 prep in,investigate
R3684 T5588 T5587 pobj mice,in
R3685 T5589 T5584 punct ", ",constructed
R3686 T5590 T5584 nsubj we,constructed
R3687 T5591 T5584 dobj animals,constructed
R3688 T5592 T5593 advmod doubly,mutant
R3689 T5593 T5591 amod mutant,animals
R3690 T5594 T5593 prep for,mutant
R3691 T5595 T5594 pobj Trip13,for
R3692 T5596 T5595 cc and,Trip13
R3693 T5597 T5595 conj Dmc1,Trip13
R3694 T5598 T5584 punct .,constructed
R3695 T5600 T5601 prep As,arrested
R3696 T5602 T5600 prep in,As
R3697 T5603 T5604 nmod Dmc1,mice
R3698 T5604 T5602 pobj mice,in
R3699 T5605 T5603 punct −,Dmc1
R3700 T5606 T5603 punct /,Dmc1
R3701 T5607 T5603 punct −,Dmc1
R3702 T5608 T5604 punct ", ",mice
R3703 T5609 T5610 prep in,undergo
R3704 T5610 T5604 relcl undergo,mice
R3705 T5611 T5609 pobj which,in
R3706 T5612 T5610 nsubj spermatocytes,undergo
R3707 T5613 T5614 amod meiotic,arrest
R3708 T5614 T5610 dobj arrest,undergo
R3709 T5615 T5610 prep from,undergo
R3710 T5616 T5617 amod defective,repair
R3711 T5617 T5615 pobj repair,from
R3712 T5618 T5617 compound DSB,repair
R3713 T5619 T5617 cc and,repair
R3714 T5620 T5621 amod failed,synapsis
R3715 T5621 T5617 conj synapsis,repair
R3716 T5622 T5621 compound chromosome,synapsis
R3717 T5623 T5624 punct [,16
R3718 T5624 T5610 parataxis 16,undergo
R3719 T5625 T5624 punct ],16
R3720 T5626 T5601 punct ", ",arrested
R3721 T5627 T5601 nsubjpass spermatogenesis,arrested
R3722 T5628 T5627 prep in,spermatogenesis
R3723 T5629 T5630 nmod Dmc1,testes
R3724 T5630 T5628 pobj testes,in
R3725 T5631 T5629 punct −,Dmc1
R3726 T5632 T5629 punct /,Dmc1
R3727 T5633 T5629 punct −,Dmc1
R3728 T5634 T5635 compound Trip13Gt,Gt
R3729 T5635 T5630 compound Gt,testes
R3730 T5636 T5635 punct /,Gt
R3731 T5637 T5601 auxpass was,arrested
R3732 T5638 T5601 advmod uniformly,arrested
R3733 T5639 T5601 prep at,arrested
R3734 T5640 T5641 det the,point
R3735 T5641 T5639 pobj point,at
R3736 T5642 T5643 advmod where,contained
R3737 T5643 T5641 relcl contained,point
R3738 T5644 T5643 nsubj spermatocytes,contained
R3739 T5645 T5643 dobj chromatin,contained
R3740 T5646 T5645 amod characteristic,chromatin
R3741 T5647 T5646 prep of,characteristic
R3742 T5648 T5649 compound zygonema,pachynema
R3743 T5649 T5647 pobj pachynema,of
R3744 T5650 T5649 punct /,pachynema
R3745 T5651 T5652 punct (,3N
R3746 T5652 T5601 parataxis 3N,arrested
R3747 T5653 T5652 compound Figure,3N
R3748 T5654 T5652 punct ),3N
R3749 T5655 T5601 punct .,arrested
R3750 T5657 T5658 amod Immunocytological,analysis
R3751 T5658 T5659 nsubj analysis,indicated
R3752 T5660 T5661 mark that,had
R3753 T5661 T5659 ccomp had,indicated
R3754 T5662 T5663 preconj both,Dmc1
R3755 T5663 T5664 nmod Dmc1,chromosomes
R3756 T5664 T5661 nsubj chromosomes,had
R3757 T5665 T5663 punct −,Dmc1
R3758 T5666 T5663 punct /,Dmc1
R3759 T5667 T5663 punct −,Dmc1
R3760 T5668 T5663 cc and,Dmc1
R3761 T5669 T5670 nmod Dmc1,Gt
R3762 T5670 T5663 conj Gt,Dmc1
R3763 T5671 T5669 punct −,Dmc1
R3764 T5672 T5669 punct /,Dmc1
R3765 T5673 T5669 punct −,Dmc1
R3766 T5674 T5670 compound Trip13Gt,Gt
R3767 T5675 T5670 punct /,Gt
R3768 T5676 T5677 amod extensive,asynapsis
R3769 T5677 T5661 dobj asynapsis,had
R3770 T5678 T5661 prep compared,had
R3771 T5679 T5678 prep to,compared
R3772 T5680 T5681 nmod Trip13Gt,mutants
R3773 T5681 T5679 pobj mutants,to
R3774 T5682 T5681 amod single,mutants
R3775 T5683 T5684 punct (,5H
R3776 T5684 T5661 parataxis 5H,had
R3777 T5685 T5684 nmod Figure,5H
R3778 T5686 T5684 nmod 5F,5H
R3779 T5687 T5684 punct –,5H
R3780 T5688 T5684 punct ),5H
R3781 T5689 T5659 punct ", ",indicated
R3782 T5690 T5659 cc and,indicated
R3783 T5691 T5692 nsubj all,had
R3784 T5692 T5659 conj had,indicated
R3785 T5693 T5694 amod persistent,foci
R3786 T5694 T5692 dobj foci,had
R3787 T5695 T5696 compound RAD51,DMC1
R3788 T5696 T5694 compound DMC1,foci
R3789 T5697 T5696 punct /,DMC1
R3790 T5698 T5694 cc and,foci
R3791 T5699 T5700 amod phosphorylated,H2AX
R3792 T5700 T5694 conj H2AX,foci
R3793 T5701 T5702 punct (,5L
R3794 T5702 T5692 parataxis 5L,had
R3795 T5703 T5702 dep γH2AX,5L
R3796 T5704 T5702 punct ;,5L
R3797 T5705 T5702 nmod Figure,5L
R3798 T5706 T5702 nmod 5I,5L
R3799 T5707 T5702 punct –,5L
R3800 T5708 T5702 punct ),5L
R3801 T5709 T5692 punct ", ",had
R3802 T5710 T5692 advcl confirming,had
R3803 T5711 T5712 mark that,is
R3804 T5712 T5710 ccomp is,confirming
R3805 T5713 T5712 nsubj Dmc1,is
R3806 T5714 T5712 acomp epistatic,is
R3807 T5715 T5714 prep to,epistatic
R3808 T5716 T5715 pobj Trip13,to
R3809 T5717 T5692 punct .,had
R3810 T5719 T5720 advmod Doubly,mutant
R3811 T5720 T5721 amod mutant,females
R3812 T5721 T5722 nsubj females,had
R3813 T5723 T5724 amod residual,ovaries
R3814 T5724 T5722 dobj ovaries,had
R3815 T5725 T5722 punct ", ",had
R3816 T5726 T5722 advcl phenocopying,had
R3817 T5727 T5728 nmod Dmc1,mutants
R3818 T5728 T5726 dobj mutants,phenocopying
R3819 T5729 T5727 punct −,Dmc1
R3820 T5730 T5727 punct /,Dmc1
R3821 T5731 T5727 punct −,Dmc1
R3822 T5732 T5727 cc and,Dmc1
R3823 T5733 T5734 compound Trip13Gt,Gt
R3824 T5734 T5727 conj Gt,Dmc1
R3825 T5735 T5734 punct /,Gt
R3826 T5736 T5728 amod single,mutants
R3827 T5737 T5738 punct (,data
R3828 T5738 T5722 meta data,had
R3829 T5739 T5738 amod unpublished,data
R3830 T5740 T5738 punct ),data
R3831 T5741 T5722 punct .,had
R3832 T5807 T5808 amod Meiotic,Defects
R3833 T5808 T5809 nsubj Defects,Are
R3834 T5810 T5808 prep in,Defects
R3835 T5811 T5812 compound Trip13Gt,Gt
R3836 T5812 T5814 compound Gt,Oocytes
R3837 T5813 T5812 punct /,Gt
R3838 T5814 T5810 pobj Oocytes,in
R3839 T5815 T5816 npadvmod DSB,Dependent
R3840 T5816 T5809 acomp Dependent,Are
R3841 T5817 T5816 punct -,Dependent
R3842 T5819 T5820 compound Epistasis,analysis
R3843 T5820 T5821 nsubj analysis,was
R3844 T5822 T5820 prep of,analysis
R3845 T5823 T5822 pobj females,of
R3846 T5824 T5821 acomp insightful,was
R3847 T5825 T5824 prep with,insightful
R3848 T5826 T5825 pobj respect,with
R3849 T5827 T5826 prep to,respect
R3850 T5828 T5829 det the,cause
R3851 T5829 T5827 pobj cause,to
R3852 T5830 T5829 prep of,cause
R3853 T5831 T5830 pobj arrest,of
R3854 T5832 T5831 prep in,arrest
R3855 T5833 T5834 compound Trip13,mutants
R3856 T5834 T5832 pobj mutants,in
R3857 T5835 T5821 punct .,was
R3858 T5837 T5838 preconj Both,Gt
R3859 T5838 T5846 nsubj Gt,had
R3860 T5839 T5838 nmod Mei1,Gt
R3861 T5840 T5839 punct −,Mei1
R3862 T5841 T5839 punct /,Mei1
R3863 T5842 T5839 punct −,Mei1
R3864 T5843 T5838 punct /,Gt
R3865 T5844 T5838 compound Trip13Gt,Gt
R3866 T5845 T5838 punct /,Gt
R3867 T5847 T5838 cc and,Gt
R3868 T5848 T5849 nmod Spo11,Gt
R3869 T5849 T5856 compound Gt,females
R3870 T5850 T5848 punct −,Spo11
R3871 T5851 T5848 punct /,Spo11
R3872 T5852 T5848 punct −,Spo11
R3873 T5853 T5849 punct /,Gt
R3874 T5854 T5849 compound Trip13Gt,Gt
R3875 T5855 T5849 punct /,Gt
R3876 T5856 T5838 conj females,Gt
R3877 T5857 T5846 dobj ovaries,had
R3878 T5858 T5857 prep with,ovaries
R3879 T5859 T5860 amod numerous,follicles
R3880 T5860 T5858 pobj follicles,with
R3881 T5861 T5857 punct ", ",ovaries
R3882 T5862 T5857 amod identical,ovaries
R3883 T5863 T5862 prep to,identical
R3884 T5864 T5865 nmod Mei1,mutants
R3885 T5865 T5863 pobj mutants,to
R3886 T5866 T5864 cc and,Mei1
R3887 T5867 T5864 conj Spo11,Mei1
R3888 T5868 T5865 amod single,mutants
R3889 T5869 T5870 punct (,3R
R3890 T5870 T5846 parataxis 3R,had
R3891 T5871 T5870 nmod Figure,3R
R3892 T5872 T5870 nmod 3O,3R
R3893 T5873 T5870 punct –,3R
R3894 T5874 T5870 punct ),3R
R3895 T5875 T5846 punct .,had
R3896 T5877 T5878 advmod Thus,are
R3897 T5879 T5878 punct ", ",are
R3898 T5880 T5878 nsubj Spo11,are
R3899 T5881 T5880 cc and,Spo11
R3900 T5882 T5880 conj Mei1,Spo11
R3901 T5883 T5878 acomp epistatic,are
R3902 T5884 T5883 prep to,epistatic
R3903 T5885 T5884 pobj Trip13,to
R3904 T5886 T5878 prep in,are
R3905 T5887 T5886 pobj oogenesis,in
R3906 T5888 T5878 punct ", ",are
R3907 T5889 T5890 advmod just,are
R3908 T5890 T5878 advcl are,are
R3909 T5891 T5890 mark as,are
R3910 T5892 T5890 nsubj they,are
R3911 T5893 T5890 acomp to,are
R3912 T5894 T5893 pobj Dmc1,to
R3913 T5895 T5896 punct [,41
R3914 T5896 T5878 parataxis 41,are
R3915 T5897 T5896 nummod 13,41
R3916 T5898 T5896 punct ",",41
R3917 T5899 T5896 punct ],41
R3918 T5900 T5878 punct .,are
R3919 T5902 T5903 nsubj This,demonstrates
R3920 T5904 T5905 mark that,is
R3921 T5905 T5903 ccomp is,demonstrates
R3922 T5906 T5907 compound oocyte,loss
R3923 T5907 T5905 nsubj loss,is
R3924 T5908 T5907 prep in,loss
R3925 T5909 T5910 compound Trip13Gt,Gt
R3926 T5910 T5912 compound Gt,females
R3927 T5911 T5910 punct /,Gt
R3928 T5912 T5908 pobj females,in
R3929 T5913 T5905 acomp dependent,is
R3930 T5914 T5913 prep on,dependent
R3931 T5915 T5916 compound DSB,formation
R3932 T5916 T5914 pobj formation,on
R3933 T5917 T5903 punct .,demonstrates
R3934 T5919 T5920 prep In,provide
R3935 T5921 T5919 pobj conjunction,In
R3936 T5922 T5921 prep with,conjunction
R3937 T5923 T5924 det the,data
R3938 T5924 T5922 pobj data,with
R3939 T5925 T5924 amod immunohistochemical,data
R3940 T5926 T5920 punct ", ",provide
R3941 T5927 T5928 det these,data
R3942 T5928 T5920 nsubj data,provide
R3943 T5929 T5930 amod strong,evidence
R3944 T5930 T5920 dobj evidence,provide
R3945 T5931 T5932 mark that,is
R3946 T5932 T5930 acl is,evidence
R3947 T5933 T5934 amod meiotic,arrest
R3948 T5934 T5932 nsubj arrest,is
R3949 T5935 T5934 prep in,arrest
R3950 T5936 T5937 compound Trip13,mice
R3951 T5937 T5935 pobj mice,in
R3952 T5938 T5937 compound mutant,mice
R3953 T5939 T5932 prep due,is
R3954 T5940 T5939 pcomp to,due
R3955 T5941 T5939 pobj defects,due
R3956 T5942 T5941 prep in,defects
R3957 T5943 T5944 compound DSB,repair
R3958 T5944 T5942 pobj repair,in
R3959 T5945 T5920 punct .,provide
R3960 T5947 T5948 mark As,expected
R3961 T5948 T5949 advcl expected,were
R3962 T5950 T5949 punct ", ",were
R3963 T5951 T5949 nsubj ovaries,were
R3964 T5952 T5951 prep of,ovaries
R3965 T5953 T5954 nmod Rec8,Trip13
R3966 T5954 T5955 nmod Trip13,mutants
R3967 T5955 T5952 pobj mutants,of
R3968 T5956 T5955 amod double,mutants
R3969 T5957 T5949 acomp devoid,were
R3970 T5958 T5957 prep of,devoid
R3971 T5959 T5958 pobj oocytes,of
R3972 T5960 T5961 mark as,were
R3973 T5961 T5949 advcl were,were
R3974 T5962 T5961 nsubj those,were
R3975 T5963 T5962 prep from,those
R3976 T5964 T5965 preconj either,mutant
R3977 T5965 T5963 pobj mutant,from
R3978 T5966 T5965 amod single,mutant
R3979 T5967 T5968 punct (,3B
R3980 T5968 T5949 parataxis 3B,were
R3981 T5969 T5968 compound Figure,3B
R3982 T5970 T5968 punct ", ",3B
R3983 T5971 T5968 conj 3S,3B
R3984 T5972 T5971 punct ", ",3S
R3985 T5973 T5971 cc and,3S
R3986 T5974 T5971 conj 3T,3S
R3987 T5975 T5968 punct ),3B
R3988 T5976 T5949 punct .,were
R6842 T10169 T10170 det The,Ortholog
R6843 T10171 T10172 compound Mouse,PCH2
R6844 T10172 T10170 compound PCH2,Ortholog
R6845 T10173 T10170 appos TRIP13,Ortholog
R6846 T10174 T10170 cc and,Ortholog
R6847 T10175 T10170 conj Expression,Ortholog
R6848 T10176 T10175 prep in,Expression
R6849 T10177 T10178 amod Wild,Type
R6850 T10178 T10176 pobj Type,in
R6851 T10179 T10178 cc and,Type
R6852 T10180 T10178 conj Mutant,Type
R6853 T10182 T10183 punct (,A
R6854 T10183 T10184 meta A,tree
R6855 T10185 T10183 punct ),A
R6856 T10186 T10184 amod Phylogenetic,tree
R6857 T10187 T10184 prep of,tree
R6858 T10188 T10189 amod presumed,orthologs
R6859 T10189 T10187 pobj orthologs,of
R6860 T10190 T10191 compound PCH2,TRIP13
R6861 T10191 T10189 compound TRIP13,orthologs
R6862 T10192 T10191 punct /,TRIP13
R6863 T10193 T10184 punct .,tree
R6864 T10195 T10196 det The,number
R6865 T10196 T10200 nsubjpass number,presented
R6866 T10197 T10198 compound database,sequence
R6867 T10198 T10199 compound sequence,accession
R6868 T10199 T10196 compound accession,number
R6869 T10201 T10196 prep of,number
R6870 T10202 T10203 det each,protein
R6871 T10203 T10201 pobj protein,of
R6872 T10204 T10200 auxpass is,presented
R6873 T10205 T10200 prep in,presented
R6874 T10206 T10207 compound Table,S1
R6875 T10207 T10205 pobj S1,in
R6876 T10208 T10200 punct .,presented
R6877 T10210 T10211 nsubj Numbers,are
R6878 T10212 T10210 acl shown,Numbers
R6879 T10213 T10214 compound bootstrap,values
R6880 T10214 T10211 attr values,are
R6881 T10215 T10216 punct (,see
R6882 T10216 T10211 parataxis see,are
R6883 T10217 T10216 dobj Materials,see
R6884 T10218 T10217 cc and,Materials
R6885 T10219 T10217 conj Methods,Materials
R6886 T10220 T10216 punct ),see
R6887 T10221 T10211 punct .,are
R6888 T10223 T10224 det The,clustering
R6889 T10224 T10225 nsubj clustering,was
R6890 T10226 T10227 det the,same
R6891 T10227 T10225 attr same,was
R6892 T10228 T10225 advmod regardless,was
R6893 T10229 T10228 prep of,regardless
R6894 T10230 T10231 mark whether,used
R6895 T10231 T10229 pcomp used,of
R6896 T10232 T10233 det the,sequence
R6897 T10233 T10231 nsubjpass sequence,used
R6898 T10234 T10235 amod whole,entire
R6899 T10235 T10233 amod entire,sequence
R6900 T10236 T10233 compound AA,sequence
R6901 T10237 T10233 cc or,sequence
R6902 T10238 T10239 amod trimmed,sequences
R6903 T10239 T10233 conj sequences,sequence
R6904 T10240 T10239 punct (,sequences
R6905 T10241 T10242 advmod where,removed
R6906 T10242 T10239 relcl removed,sequences
R6907 T10243 T10242 nsubjpass regions,removed
R6908 T10244 T10243 acl showing,regions
R6909 T10245 T10246 amod little,conservation
R6910 T10246 T10244 dobj conservation,showing
R6911 T10247 T10242 auxpass were,removed
R6912 T10248 T10231 punct ),used
R6913 T10249 T10231 auxpass were,used
R6914 T10250 T10231 prep for,used
R6915 T10251 T10252 det the,analysis
R6916 T10252 T10250 pobj analysis,for
R6917 T10253 T10225 punct .,was
R6918 T10255 T10256 amod Major,groups
R6919 T10256 T10258 nsubjpass groups,indicated
R6920 T10257 T10256 amod eukaryotic,groups
R6921 T10259 T10258 auxpass are,indicated
R6922 T10260 T10258 prep in,indicated
R6923 T10261 T10260 pobj color,in
R6924 T10262 T10258 punct ", ",indicated
R6925 T10263 T10264 mark with,in
R6926 T10264 T10258 advcl in,indicated
R6927 T10265 T10264 nsubj deuterostomia,in
R6928 T10266 T10264 pobj blue,in
R6929 T10267 T10264 punct ", ",in
R6930 T10268 T10269 nsubj plants,in
R6931 T10269 T10264 conj in,in
R6932 T10270 T10269 pobj green,in
R6933 T10271 T10269 punct ", ",in
R6934 T10272 T10273 nsubj protostomia,in
R6935 T10273 T10269 conj in,in
R6936 T10274 T10273 pobj purple,in
R6937 T10275 T10273 punct ", ",in
R6938 T10276 T10273 cc and,in
R6939 T10277 T10278 nsubj fungi,in
R6940 T10278 T10273 conj in,in
R6941 T10279 T10278 pobj maroon,in
R6942 T10280 T10258 punct .,indicated
R6943 T10282 T10283 punct (,B
R6944 T10283 T10284 meta B,products
R6945 T10285 T10283 punct ),B
R6946 T10286 T10284 compound Amplification,products
R6947 T10287 T10284 prep of,products
R6948 T10288 T10287 pobj cDNA,of
R6949 T10289 T10284 prep from,products
R6950 T10290 T10291 det the,tissues
R6951 T10291 T10289 pobj tissues,from
R6952 T10292 T10291 amod following,tissues
R6953 T10293 T10291 punct : ,tissues
R6954 T10294 T10295 meta 1,heart
R6955 T10295 T10291 appos heart,tissues
R6956 T10296 T10295 punct ", ",heart
R6957 T10297 T10295 punct ;,heart
R6958 T10298 T10299 meta 2,brain
R6959 T10299 T10295 conj brain,heart
R6960 T10300 T10299 punct ", ",brain
R6961 T10301 T10299 punct ;,brain
R6962 T10302 T10303 meta 3,spleen
R6963 T10303 T10299 conj spleen,brain
R6964 T10304 T10303 punct ", ",spleen
R6965 T10305 T10303 punct ;,spleen
R6966 T10306 T10307 meta 4,lung
R6967 T10307 T10303 conj lung,spleen
R6968 T10308 T10307 punct ", ",lung
R6969 T10309 T10307 punct ;,lung
R6970 T10310 T10311 meta 5,liver
R6971 T10311 T10307 conj liver,lung
R6972 T10312 T10311 punct ", ",liver
R6973 T10313 T10311 punct ;,liver
R6974 T10314 T10315 meta 6,muscle
R6975 T10315 T10311 conj muscle,liver
R6976 T10316 T10315 punct ", ",muscle
R6977 T10317 T10315 amod skeletal,muscle
R6978 T10318 T10315 punct ;,muscle
R6979 T10319 T10320 meta 7,kidney
R6980 T10320 T10315 conj kidney,muscle
R6981 T10321 T10320 punct ", ",kidney
R6982 T10322 T10320 punct ;,kidney
R6983 T10323 T10324 meta 8,testis
R6984 T10324 T10320 conj testis,kidney
R6985 T10325 T10324 punct ", ",testis
R6986 T10326 T10324 punct ;,testis
R6987 T10327 T10328 meta 9,embryo
R6988 T10328 T10324 conj embryo,testis
R6989 T10329 T10328 punct ", ",embryo
R6990 T10330 T10328 compound E7,embryo
R6991 T10331 T10328 punct ;,embryo
R6992 T10332 T10333 meta 10,embryo
R6993 T10333 T10328 conj embryo,embryo
R6994 T10334 T10333 punct ", ",embryo
R6995 T10335 T10333 compound E11,embryo
R6996 T10336 T10333 punct ;,embryo
R6997 T10337 T10338 meta 11,embryo
R6998 T10338 T10333 conj embryo,embryo
R6999 T10339 T10338 punct ", ",embryo
R7000 T10340 T10338 compound E15,embryo
R7001 T10341 T10338 punct ;,embryo
R7002 T10342 T10338 cc and,embryo
R7003 T10343 T10344 meta 12,embryo
R7004 T10344 T10338 conj embryo,embryo
R7005 T10345 T10344 punct ", ",embryo
R7006 T10346 T10344 compound E17,embryo
R7007 T10347 T10284 punct .,products
R7008 T10349 T10350 punct (,C
R7009 T10350 T10351 meta C,structure
R7010 T10352 T10350 punct ),C
R7011 T10353 T10354 compound Intron,exon
R7012 T10354 T10351 compound exon,structure
R7013 T10355 T10354 punct –,exon
R7014 T10356 T10351 prep of,structure
R7015 T10357 T10356 pobj TRIP13,of
R7016 T10358 T10351 cc and,structure
R7017 T10359 T10360 compound insertion,site
R7018 T10360 T10351 conj site,structure
R7019 T10361 T10360 prep of,site
R7020 T10362 T10363 compound gene,trap
R7021 T10363 T10365 compound trap,vector
R7022 T10364 T10363 punct -,trap
R7023 T10365 T10361 pobj vector,of
R7024 T10366 T10360 punct .,site
R7025 T10369 T10368 dobj Materials,See
R7026 T10370 T10369 cc and,Materials
R7027 T10371 T10369 conj Methods,Materials
R7028 T10372 T10368 prep for,See
R7029 T10373 T10372 pobj details,for
R7030 T10374 T10373 prep on,details
R7031 T10375 T10376 advmod how,identified
R7032 T10376 T10374 pcomp identified,on
R7033 T10377 T10378 det the,site
R7034 T10378 T10376 nsubjpass site,identified
R7035 T10379 T10378 amod precise,site
R7036 T10380 T10378 compound insertion,site
R7037 T10381 T10376 auxpass was,identified
R7038 T10382 T10368 punct .,See
R7039 T10384 T10385 punct (,D
R7040 T10385 T10386 meta D,PCR
R7041 T10387 T10385 punct ),D
R7042 T10388 T10386 compound RT,PCR
R7043 T10389 T10386 punct -,PCR
R7044 T10390 T10386 prep of,PCR
R7045 T10391 T10390 pobj Trip13,of
R7046 T10392 T10391 cc and,Trip13
R7047 T10393 T10394 det a,Med31
R7048 T10394 T10391 conj Med31,Trip13
R7049 T10395 T10394 compound control,Med31
R7050 T10396 T10394 compound gene,Med31
R7051 T10397 T10394 prep from,Med31
R7052 T10398 T10399 compound testis,RNA
R7053 T10399 T10397 pobj RNA,from
R7054 T10400 T10386 punct .,PCR
R7055 T10402 T10403 det The,primers
R7056 T10403 T10405 nsubjpass primers,situated
R7057 T10404 T10403 compound Trip13,primers
R7058 T10406 T10405 auxpass are,situated
R7059 T10407 T10405 prep in,situated
R7060 T10408 T10409 det the,exons
R7061 T10409 T10407 pobj exons,in
R7062 T10410 T10409 amod first,exons
R7063 T10411 T10410 cc and,first
R7064 T10412 T10410 conj last,first
R7065 T10413 T10414 punct (,see
R7066 T10414 T10405 parataxis see,situated
R7067 T10415 T10414 dobj Materials,see
R7068 T10416 T10415 cc and,Materials
R7069 T10417 T10415 conj Methods,Materials
R7070 T10418 T10414 punct ),see
R7071 T10419 T10405 punct .,situated
R7072 T10421 T10422 punct (,E
R7073 T10422 T10423 meta E,analysis
R7074 T10424 T10422 punct ),E
R7075 T10425 T10426 compound Western,blot
R7076 T10426 T10423 compound blot,analysis
R7077 T10427 T10423 prep of,analysis
R7078 T10428 T10429 compound testis,protein
R7079 T10429 T10427 pobj protein,of
R7080 T10430 T10423 prep with,analysis
R7081 T10431 T10432 amod anti-TRIP13,antibody
R7082 T10432 T10430 pobj antibody,with
R7083 T10433 T10423 punct .,analysis
R7084 T10435 T10436 det The,blot
R7085 T10436 T10437 nsubjpass blot,probed
R7086 T10438 T10437 auxpass was,probed
R7087 T10439 T10437 advmod later,probed
R7088 T10440 T10437 prep with,probed
R7089 T10441 T10442 amod anti-alpha,actin
R7090 T10442 T10440 pobj actin,with
R7091 T10443 T10442 compound tubulin,actin
R7092 T10444 T10442 prep as,actin
R7093 T10445 T10446 det a,control
R7094 T10446 T10444 pobj control,as
R7095 T10447 T10446 compound loading,control
R7096 T10448 T10437 punct .,probed
R7097 T10450 T10451 det The,protein
R7098 T10451 T10454 nsubj protein,is
R7099 T10452 T10451 amod expected,protein
R7100 T10453 T10451 compound TRIP13,protein
R7101 T10455 T10456 punct ∼,48
R7102 T10456 T10457 nummod 48,KDa
R7103 T10457 T10454 attr KDa,is
R7104 T10458 T10454 punct .,is
R7105 T10460 T10461 punct (,F
R7106 T10461 T10462 meta F,Localization
R7107 T10463 T10461 punct ),F
R7108 T10464 T10462 prep of,Localization
R7109 T10465 T10464 pobj TRIP13,of
R7110 T10466 T10462 prep in,Localization
R7111 T10467 T10466 pobj testes,in
R7112 T10468 T10462 punct .,Localization
R7113 T10470 T10471 amod Wild,type
R7114 T10471 T10473 nmod type,testis
R7115 T10472 T10471 punct -,type
R7116 T10473 T10482 compound testis,sections
R7117 T10474 T10475 punct (,top
R7118 T10475 T10471 parataxis top,type
R7119 T10476 T10475 punct ),top
R7120 T10477 T10471 cc and,type
R7121 T10478 T10471 conj mutant,type
R7122 T10479 T10480 punct (,bottom
R7123 T10480 T10478 parataxis bottom,mutant
R7124 T10481 T10480 punct ),bottom
R7125 T10482 T10483 nsubjpass sections,probed
R7126 T10484 T10483 auxpass were,probed
R7127 T10485 T10483 prep with,probed
R7128 T10486 T10485 pobj chicken,with
R7129 T10487 T10486 amod anti-TRIP13,chicken
R7130 T10488 T10483 punct ", ",probed
R7131 T10489 T10483 cc and,probed
R7132 T10490 T10483 conj detected,probed
R7133 T10491 T10490 prep with,detected
R7134 T10492 T10493 npadvmod HRP,conjugated
R7135 T10493 T10495 amod conjugated,IgG
R7136 T10494 T10493 punct -,conjugated
R7137 T10495 T10491 pobj IgG,with
R7138 T10496 T10495 amod anti-chicken,IgG
R7139 T10497 T10498 punct (,staining
R7140 T10498 T10495 parataxis staining,IgG
R7141 T10499 T10500 amod brown,red
R7142 T10500 T10498 amod red,staining
R7143 T10501 T10500 punct /,red
R7144 T10502 T10498 punct ),staining
R7145 T10503 T10483 punct .,probed
R7146 T10505 T10506 nsubj Expression,was
R7147 T10507 T10505 prep in,Expression
R7148 T10508 T10507 pobj WT,in
R7149 T10509 T10510 advmod most,prominent
R7150 T10510 T10506 acomp prominent,was
R7151 T10511 T10506 prep in,was
R7152 T10512 T10513 det the,nuclei
R7153 T10513 T10511 pobj nuclei,in
R7154 T10514 T10513 prep of,nuclei
R7155 T10515 T10516 compound Type,B
R7156 T10516 T10517 compound B,spermatogonia
R7157 T10517 T10514 pobj spermatogonia,of
R7158 T10518 T10517 punct (,spermatogonia
R7159 T10519 T10517 appos Sg,spermatogonia
R7160 T10520 T10517 punct ),spermatogonia
R7161 T10521 T10517 punct ", ",spermatogonia
R7162 T10522 T10523 compound leptotene,spermatocytes
R7163 T10523 T10517 conj spermatocytes,spermatogonia
R7164 T10524 T10523 punct (,spermatocytes
R7165 T10525 T10523 appos LS,spermatocytes
R7166 T10526 T10523 punct ),spermatocytes
R7167 T10527 T10523 punct ", ",spermatocytes
R7168 T10528 T10523 cc and,spermatocytes
R7169 T10529 T10530 amod early,spermatocytes
R7170 T10530 T10523 conj spermatocytes,spermatocytes
R7171 T10531 T10530 compound pachytene,spermatocytes
R7172 T10532 T10530 punct (,spermatocytes
R7173 T10533 T10530 appos PS,spermatocytes
R7174 T10534 T10530 punct ),spermatocytes
R7175 T10535 T10530 punct ", ",spermatocytes
R7176 T10536 T10530 cc but,spermatocytes
R7177 T10537 T10536 neg not,but
R7178 T10538 T10539 amod late,spermatocytes
R7179 T10539 T10530 conj spermatocytes,spermatocytes
R7180 T10540 T10539 compound pachytene,spermatocytes
R7181 T10541 T10539 punct (,spermatocytes
R7182 T10542 T10539 appos LP,spermatocytes
R7183 T10543 T10506 punct ),was
R7184 T10544 T10506 punct .,was
R7185 T10546 T10547 det No,staining
R7186 T10547 T10549 nsubjpass staining,seen
R7187 T10548 T10547 amod nuclear,staining
R7188 T10550 T10549 auxpass was,seen
R7189 T10551 T10549 prep in,seen
R7190 T10552 T10553 compound mutant,sections
R7191 T10553 T10551 pobj sections,in
R7192 T10554 T10553 compound testis,sections
R7193 T10555 T10549 punct ", ",seen
R7194 T10556 T10557 mark although,is
R7195 T10557 T10549 advcl is,seen
R7196 T10558 T10559 amod reddish,background
R7197 T10559 T10557 nsubj background,is
R7198 T10560 T10559 amod cytoplasmic,background
R7199 T10561 T10557 acomp present,is
R7200 T10562 T10549 punct .,seen
R7201 T10564 T10565 nsubjpass Identification,judged
R7202 T10566 T10564 prep of,Identification
R7203 T10567 T10568 compound cell,types
R7204 T10568 T10566 pobj types,of
R7205 T10569 T10565 auxpass was,judged
R7206 T10570 T10565 prep in,judged
R7207 T10571 T10570 amod part,in
R7208 T10572 T10565 prep by,judged
R7209 T10573 T10572 pcomp estimating,by
R7210 T10574 T10575 det the,stage
R7211 T10575 T10573 dobj stage,estimating
R7212 T10576 T10575 amod epithelial,stage
R7213 T10577 T10575 prep of,stage
R7214 T10578 T10579 det the,tubules
R7215 T10579 T10577 pobj tubules,of
R7216 T10580 T10581 mark as,described
R7217 T10581 T10573 advcl described,estimating
R7218 T10582 T10583 punct [,67
R7219 T10583 T10573 parataxis 67,estimating
R7220 T10584 T10583 punct ],67
R7221 T10585 T10565 punct .,judged
R7222 T10587 T10588 punct (,G
R7223 T10588 T10589 meta G,localization
R7224 T10590 T10588 punct ),G
R7225 T10591 T10589 compound TRIP13,localization
R7226 T10592 T10589 prep in,localization
R7227 T10593 T10594 compound surface,spread
R7228 T10594 T10596 compound spread,spermatocytes
R7229 T10595 T10594 punct -,spread
R7230 T10596 T10592 pobj spermatocytes,in
R7231 T10597 T10589 punct .,localization
R7232 T10599 T10600 nsubjpass Preparations,immunolabeled
R7233 T10601 T10600 auxpass were,immunolabeled
R7234 T10602 T10600 prep with,immunolabeled
R7235 T10603 T10602 pobj anti-SYCP3,with
R7236 T10604 T10603 punct (,anti-SYCP3
R7237 T10605 T10603 appos S,anti-SYCP3
R7238 T10606 T10603 punct ),anti-SYCP3
R7239 T10607 T10603 cc and,anti-SYCP3
R7240 T10608 T10603 conj TRIP13,anti-SYCP3
R7241 T10609 T10608 punct (,TRIP13
R7242 T10610 T10608 appos T,TRIP13
R7243 T10611 T10600 punct ),immunolabeled
R7244 T10612 T10600 punct .,immunolabeled
R7245 T10614 T10615 preconj Both,individual
R7246 T10615 T10616 amod individual,images
R7247 T10616 T10619 nsubjpass images,shown
R7248 T10617 T10615 cc and,individual
R7249 T10618 T10615 conj merged,individual
R7250 T10620 T10619 auxpass are,shown
R7251 T10621 T10619 prep for,shown
R7252 T10622 T10623 nmod leptotene,spermatocytes
R7253 T10623 T10621 pobj spermatocytes,for
R7254 T10624 T10622 punct (,leptotene
R7255 T10625 T10622 appos Lep,leptotene
R7256 T10626 T10622 punct ),leptotene
R7257 T10627 T10622 punct ", ",leptotene
R7258 T10628 T10622 conj zygotene,leptotene
R7259 T10629 T10628 punct (,zygotene
R7260 T10630 T10628 appos Zyg,zygotene
R7261 T10631 T10628 punct ),zygotene
R7262 T10632 T10628 punct ", ",zygotene
R7263 T10633 T10628 cc and,zygotene
R7264 T10634 T10628 conj pachytene,zygotene
R7265 T10635 T10634 punct (,pachytene
R7266 T10636 T10634 appos Pac,pachytene
R7267 T10637 T10623 punct ),spermatocytes
R7268 T10638 T10619 punct .,shown
R7269 T10640 T10641 amod Nuclear,staining
R7270 T10641 T10642 nsubj staining,was
R7271 T10643 T10642 acomp absent,was
R7272 T10644 T10642 prep in,was
R7273 T10645 T10646 det the,mutant
R7274 T10646 T10644 pobj mutant,in
R7275 T10647 T10642 punct .,was
R7276 T10698 T10699 amod Developmental,Phenotypes
R7277 T10700 T10699 prep of,Phenotypes
R7278 T10701 T10702 compound Trip13,Mutant
R7279 T10702 T10703 compound Mutant,Mice
R7280 T10703 T10700 pobj Mice,of
R7281 T10705 T10706 punct (,A
R7282 T10706 T10707 meta A,are
R7283 T10708 T10706 punct ),A
R7284 T10709 T10707 dep Shown,are
R7285 T10710 T10711 nummod 21,d
R7286 T10711 T10713 npadvmod d,old
R7287 T10712 T10711 punct -,d
R7288 T10713 T10715 amod old,littermates
R7289 T10714 T10713 punct -,old
R7290 T10715 T10707 nsubj littermates,are
R7291 T10716 T10707 punct .,are
R7292 T10719 T10720 det the,tail
R7293 T10720 T10718 dobj tail,Note
R7294 T10721 T10720 amod shortened,tail
R7295 T10722 T10720 prep in,tail
R7296 T10723 T10724 det the,mutant
R7297 T10724 T10722 pobj mutant,in
R7298 T10725 T10720 punct ", ",tail
R7299 T10726 T10720 cc but,tail
R7300 T10727 T10728 amod overall,similar
R7301 T10728 T10729 amod similar,size
R7302 T10729 T10720 conj size,tail
R7303 T10730 T10729 compound body,size
R7304 T10731 T10718 punct .,Note
R7305 T10733 T10734 punct (,B
R7306 T10734 T10735 meta B,are
R7307 T10736 T10734 punct ),B
R7308 T10737 T10735 dep Shown,are
R7309 T10738 T10739 nummod 23,d
R7310 T10739 T10741 npadvmod d,old
R7311 T10740 T10739 punct -,d
R7312 T10741 T10743 amod old,littermates
R7313 T10742 T10741 punct -,old
R7314 T10743 T10735 nsubj littermates,are
R7315 T10744 T10735 punct .,are
R7316 T10746 T10747 det The,mutant
R7317 T10747 T10748 nsubj mutant,is
R7318 T10749 T10748 acomp smaller,is
R7319 T10750 T10748 prep in,is
R7320 T10751 T10752 det this,case
R7321 T10752 T10750 pobj case,in
R7322 T10753 T10748 punct ", ",is
R7323 T10754 T10748 cc but,is
R7324 T10755 T10756 det the,tail
R7325 T10756 T10757 nsubj tail,is
R7326 T10757 T10748 conj is,is
R7327 T10758 T10757 neg not,is
R7328 T10759 T10760 advmod as,truncated
R7329 T10760 T10757 acomp truncated,is
R7330 T10761 T10760 prep as,truncated
R7331 T10762 T10763 det the,mouse
R7332 T10763 T10761 pobj mouse,as
R7333 T10764 T10763 prep in,mouse
R7334 T10765 T10766 punct (,A
R7335 T10766 T10764 pobj A,in
R7336 T10767 T10766 punct ),A
R7337 T10768 T10757 punct .,is
R7338 T10770 T10771 punct (,C
R7339 T10771 T10772 meta C,testes
R7340 T10773 T10771 punct ),C
R7341 T10774 T10775 amod Wild,type
R7342 T10775 T10772 nmod type,testes
R7343 T10776 T10775 punct -,type
R7344 T10777 T10775 punct (,type
R7345 T10778 T10775 appos WT,type
R7346 T10779 T10775 punct ),type
R7347 T10780 T10775 cc and,type
R7348 T10781 T10782 amod homozygous,mutant
R7349 T10782 T10775 conj mutant,type
R7350 T10783 T10782 compound Trip13,mutant
R7351 T10784 T10782 punct (,mutant
R7352 T10785 T10782 appos MUT,mutant
R7353 T10786 T10772 punct ),testes
R7354 T10787 T10772 punct .,testes
R7355 T10789 T10790 punct (,D
R7356 T10790 T10791 nsubj D,are
R7357 T10792 T10790 punct ),D
R7358 T10793 T10790 cc and,D
R7359 T10794 T10795 punct (,E
R7360 T10795 T10790 conj E,D
R7361 T10796 T10795 punct ),E
R7362 T10797 T10798 compound cross,sections
R7363 T10798 T10791 attr sections,are
R7364 T10799 T10798 prep through,sections
R7365 T10800 T10801 nummod 17.5,d
R7366 T10801 T10803 npadvmod d,old
R7367 T10802 T10801 punct -,d
R7368 T10803 T10805 amod old,testes
R7369 T10804 T10803 punct -,old
R7370 T10805 T10799 pobj testes,through
R7371 T10806 T10807 amod heterozygous,mutant
R7372 T10807 T10805 compound mutant,testes
R7373 T10808 T10809 punct (,WT
R7374 T10809 T10806 parataxis WT,heterozygous
R7375 T10810 T10809 punct “,WT
R7376 T10811 T10809 punct ”,WT
R7377 T10812 T10809 punct ),WT
R7378 T10813 T10806 cc and,heterozygous
R7379 T10814 T10806 conj homozygous,heterozygous
R7380 T10815 T10805 compound Trip13,testes
R7381 T10816 T10791 punct ", ",are
R7382 T10817 T10791 advmod respectively,are
R7383 T10818 T10791 punct .,are
R7384 T10820 T10821 mark Whereas,show
R7385 T10821 T10826 advcl show,synchronized
R7386 T10822 T10823 det the,tubules
R7387 T10823 T10821 nsubj tubules,show
R7388 T10824 T10823 prep in,tubules
R7389 T10825 T10824 pobj WT,in
R7390 T10827 T10828 amod coordinated,spermatogenesis
R7391 T10828 T10821 dobj spermatogenesis,show
R7392 T10829 T10828 prep with,spermatogenesis
R7393 T10830 T10831 compound pachytene,spermatocytes
R7394 T10831 T10829 pobj spermatocytes,with
R7395 T10832 T10831 relcl present,spermatocytes
R7396 T10833 T10832 prep in,present
R7397 T10834 T10835 det all,tubules
R7398 T10835 T10833 pobj tubules,in
R7399 T10836 T10835 punct (,tubules
R7400 T10837 T10835 amod proximal,tubules
R7401 T10838 T10837 prep to,proximal
R7402 T10839 T10840 det the,lumen
R7403 T10840 T10838 pobj lumen,to
R7404 T10841 T10826 punct ),synchronized
R7405 T10842 T10826 punct ", ",synchronized
R7406 T10843 T10844 amod developmental,progression
R7407 T10844 T10826 nsubjpass progression,synchronized
R7408 T10845 T10844 prep in,progression
R7409 T10846 T10847 det the,mutant
R7410 T10847 T10845 pobj mutant,in
R7411 T10848 T10826 auxpass is,synchronized
R7412 T10849 T10826 neg not,synchronized
R7413 T10850 T10826 prep between,synchronized
R7414 T10851 T10850 pobj tubules,between
R7415 T10852 T10826 punct .,synchronized
R7416 T10854 T10855 det Some,tubules
R7417 T10855 T10856 nsubj tubules,have
R7418 T10857 T10858 det no,spermatocyes
R7419 T10858 T10856 dobj spermatocyes,have
R7420 T10859 T10858 compound pachytene,spermatocyes
R7421 T10860 T10861 punct (,asterisks
R7422 T10861 T10858 parataxis asterisks,spermatocyes
R7423 T10862 T10861 punct ),asterisks
R7424 T10863 T10856 punct ", ",have
R7425 T10864 T10865 mark while,is
R7426 T10865 T10856 advcl is,have
R7427 T10866 T10865 prep in,is
R7428 T10867 T10866 pobj others,in
R7429 T10868 T10865 punct ", ",is
R7430 T10869 T10865 nsubj development,is
R7431 T10870 T10871 advmod somewhat,disorganized
R7432 T10871 T10865 acomp disorganized,is
R7433 T10872 T10873 punct (,#
R7434 T10873 T10865 punct #,is
R7435 T10874 T10873 punct ),#
R7436 T10875 T10856 punct .,have
R7437 T11058 T11057 prep of,Histology
R7438 T11059 T11060 compound Mutant,Gonads
R7439 T11060 T11058 pobj Gonads,of
R7440 T11062 T11063 nsubj All,are
R7441 T11064 T11065 compound hematoxylin,eosin
R7442 T11065 T11067 npadvmod eosin,stained
R7443 T11066 T11065 punct /,eosin
R7444 T11067 T11069 amod stained,sections
R7445 T11068 T11067 punct -,stained
R7446 T11069 T11063 attr sections,are
R7447 T11070 T11069 compound paraffin,sections
R7448 T11071 T11063 punct .,are
R7449 T11073 T11074 nsubj Testes,are
R7450 T11075 T11074 prep from,are
R7451 T11076 T11077 nummod 6,wk
R7452 T11077 T11079 npadvmod wk,old
R7453 T11078 T11077 punct -,wk
R7454 T11079 T11081 amod old,males
R7455 T11080 T11079 punct -,old
R7456 T11081 T11075 pobj males,from
R7457 T11082 T11074 punct ", ",are
R7458 T11083 T11074 prep except,are
R7459 T11084 T11085 mark as,indicated
R7460 T11085 T11083 pcomp indicated,except
R7461 T11086 T11085 advmod below,indicated
R7462 T11087 T11074 punct .,are
R7463 T11089 T11090 punct (,A
R7464 T11090 T11091 meta A,ovary
R7465 T11092 T11090 punct ),A
R7466 T11093 T11094 amod Wild,type
R7467 T11094 T11091 nmod type,ovary
R7468 T11095 T11094 punct -,type
R7469 T11096 T11097 nummod 25,d
R7470 T11097 T11099 npadvmod d,old
R7471 T11098 T11097 punct -,d
R7472 T11099 T11091 amod old,ovary
R7473 T11100 T11099 punct -,old
R7474 T11101 T11091 punct .,ovary
R7475 T11103 T11104 punct (,B
R7476 T11104 T11105 meta B,ovary
R7477 T11106 T11104 punct ),B
R7478 T11107 T11108 nmod Trip13Gt,Gt
R7479 T11108 T11105 nmod Gt,ovary
R7480 T11109 T11108 punct /,Gt
R7481 T11110 T11111 nummod 25,d
R7482 T11111 T11113 npadvmod d,old
R7483 T11112 T11111 punct -,d
R7484 T11113 T11105 amod old,ovary
R7485 T11114 T11113 punct -,old
R7486 T11115 T11105 punct ", ",ovary
R7487 T11116 T11105 acl showing,ovary
R7488 T11117 T11116 dobj dysgenesis,showing
R7489 T11118 T11117 prep from,dysgenesis
R7490 T11119 T11120 det an,absence
R7491 T11120 T11118 pobj absence,from
R7492 T11121 T11120 prep of,absence
R7493 T11122 T11121 pobj oocytes,of
R7494 T11123 T11105 punct .,ovary
R7495 T11125 T11126 punct (,C
R7496 T11126 T11127 meta C,ovary
R7497 T11128 T11126 punct ),C
R7498 T11129 T11127 nmod Trip13Gt,ovary
R7499 T11130 T11129 punct /,Trip13Gt
R7500 T11131 T11129 punct +,Trip13Gt
R7501 T11132 T11133 nummod 2,d
R7502 T11133 T11135 npadvmod d,old
R7503 T11134 T11133 punct -,d
R7504 T11135 T11127 amod old,ovary
R7505 T11136 T11135 punct -,old
R7506 T11137 T11127 compound control,ovary
R7507 T11138 T11127 punct .,ovary
R7508 T11140 T11141 nsubj Arrows,point
R7509 T11142 T11141 prep to,point
R7510 T11143 T11142 pobj oocytes,to
R7511 T11144 T11143 prep in,oocytes
R7512 T11145 T11146 advmod newly,forming
R7513 T11146 T11147 amod forming,follicles
R7514 T11147 T11144 pobj follicles,in
R7515 T11148 T11141 punct .,point
R7516 T11150 T11151 punct (,D
R7517 T11151 T11152 meta D,ovary
R7518 T11153 T11151 punct ),D
R7519 T11154 T11155 nmod Trip13Gt,Gt
R7520 T11155 T11152 nmod Gt,ovary
R7521 T11156 T11155 punct /,Gt
R7522 T11157 T11158 nummod 2,d
R7523 T11158 T11160 npadvmod d,old
R7524 T11159 T11158 punct -,d
R7525 T11160 T11152 amod old,ovary
R7526 T11161 T11160 punct -,old
R7527 T11162 T11152 punct ", ",ovary
R7528 T11163 T11152 relcl dysgenic,ovary
R7529 T11164 T11163 prep due,dysgenic
R7530 T11165 T11164 pcomp to,due
R7531 T11166 T11164 pobj lack,due
R7532 T11167 T11166 prep of,lack
R7533 T11168 T11167 pobj oocytes,of
R7534 T11169 T11152 punct .,ovary
R7535 T11171 T11172 nsubj Magnification,is
R7536 T11173 T11174 det the,same
R7537 T11174 T11172 attr same,is
R7538 T11175 T11174 prep as,same
R7539 T11176 T11177 poss its,littermate
R7540 T11177 T11175 pobj littermate,as
R7541 T11178 T11177 prep in,littermate
R7542 T11179 T11180 punct “,C.
R7543 T11180 T11178 pobj C.,in
R7544 T11181 T11172 punct ”,is
R7545 T11183 T11184 punct (,E
R7546 T11184 T11185 meta E,testis
R7547 T11186 T11184 punct ),E
R7548 T11187 T11188 amod Wild,type
R7549 T11188 T11185 compound type,testis
R7550 T11189 T11188 punct -,type
R7551 T11190 T11185 punct .,testis
R7552 T11192 T11193 punct (,F
R7553 T11193 T11194 meta F,testis
R7554 T11195 T11193 punct ),F
R7555 T11196 T11197 compound Trip13Gt,Gt
R7556 T11197 T11194 compound Gt,testis
R7557 T11198 T11197 punct /,Gt
R7558 T11199 T11194 prep with,testis
R7559 T11200 T11201 amod uniform,arrest
R7560 T11201 T11199 pobj arrest,with
R7561 T11202 T11201 compound pachytene,arrest
R7562 T11203 T11194 punct .,testis
R7563 T11205 T11206 punct (,G
R7564 T11206 T11207 meta G,testis
R7565 T11208 T11206 punct ),G
R7566 T11209 T11210 nmod Trip13Gt,Gt
R7567 T11210 T11207 nmod Gt,testis
R7568 T11211 T11210 punct /,Gt
R7569 T11212 T11213 nummod 3,mo
R7570 T11213 T11215 npadvmod mo,old
R7571 T11214 T11213 punct -,mo
R7572 T11215 T11207 amod old,testis
R7573 T11216 T11215 punct -,old
R7574 T11217 T11207 prep with,testis
R7575 T11218 T11219 det some,spermatids
R7576 T11219 T11217 pobj spermatids,with
R7577 T11220 T11219 amod postmeiotic,spermatids
R7578 T11221 T11222 punct (,arrows
R7579 T11222 T11219 parataxis arrows,spermatids
R7580 T11223 T11222 punct ),arrows
R7581 T11224 T11207 punct .,testis
R7582 T11226 T11227 punct (,H
R7583 T11227 T11228 meta H,testis
R7584 T11229 T11227 punct ),H
R7585 T11230 T11228 nmod Spo11,testis
R7586 T11231 T11230 punct −,Spo11
R7587 T11232 T11230 punct /,Spo11
R7588 T11233 T11230 punct -,Spo11
R7589 T11234 T11228 punct .,testis
R7590 T11236 T11237 det A,tubule
R7591 T11237 T11238 nsubjpass tubule,labeled
R7592 T11239 T11237 prep with,tubule
R7593 T11240 T11239 pobj spermatocytes,with
R7594 T11241 T11240 prep at,spermatocytes
R7595 T11242 T11243 compound leptotene,zygotene
R7596 T11243 T11245 compound zygotene,transition
R7597 T11244 T11243 punct /,zygotene
R7598 T11245 T11241 pobj transition,at
R7599 T11246 T11238 auxpass is,labeled
R7600 T11247 T11238 oprd ZP,labeled
R7601 T11248 T11238 punct ", ",labeled
R7602 T11249 T11238 cc and,labeled
R7603 T11250 T11251 nsubjpass tubules,marked
R7604 T11251 T11238 conj marked,labeled
R7605 T11252 T11250 prep with,tubules
R7606 T11253 T11254 amod apoptotic,spermatocytes
R7607 T11254 T11252 pobj spermatocytes,with
R7608 T11255 T11251 auxpass are,marked
R7609 T11256 T11251 prep with,marked
R7610 T11257 T11258 det an,asterisk
R7611 T11258 T11256 pobj asterisk,with
R7612 T11259 T11251 punct .,marked
R7613 T11261 T11262 det The,specimen
R7614 T11262 T11263 nsubjpass specimen,taken
R7615 T11264 T11263 auxpass was,taken
R7616 T11265 T11263 prep from,taken
R7617 T11266 T11267 det a,littermate
R7618 T11267 T11265 pobj littermate,from
R7619 T11268 T11267 prep of,littermate
R7620 T11269 T11268 pobj that,of
R7621 T11270 T11269 prep in,that
R7622 T11271 T11272 punct (,I
R7623 T11272 T11270 pobj I,in
R7624 T11273 T11272 punct ),I
R7625 T11274 T11263 punct .,taken
R7626 T11276 T11277 punct (,I
R7627 T11277 T11278 meta I,testis
R7628 T11279 T11277 punct ),I
R7629 T11280 T11278 nmod Spo11,testis
R7630 T11281 T11280 punct −,Spo11
R7631 T11282 T11280 punct /,Spo11
R7632 T11283 T11280 punct −,Spo11
R7633 T11284 T11285 compound Trip13Gt,Gt
R7634 T11285 T11278 compound Gt,testis
R7635 T11286 T11285 punct /,Gt
R7636 T11287 T11278 punct .,testis
R7637 T11289 T11290 nsubj Labeling,is
R7638 T11291 T11292 advmod the,same
R7639 T11292 T11290 acomp same,is
R7640 T11293 T11292 prep as,same
R7641 T11294 T11293 prep in,as
R7642 T11295 T11296 punct (,H
R7643 T11296 T11294 pobj H,in
R7644 T11297 T11296 punct ),H
R7645 T11298 T11290 punct .,is
R7646 T11300 T11301 det The,inset
R7647 T11301 T11302 nsubj inset,contains
R7648 T11303 T11304 det a,tubule
R7649 T11304 T11302 dobj tubule,contains
R7650 T11305 T11304 prep with,tubule
R7651 T11306 T11307 compound leptotene,zygotene
R7652 T11307 T11309 compound zygotene,spermatocytes
R7653 T11308 T11307 punct -,zygotene
R7654 T11309 T11305 pobj spermatocytes,with
R7655 T11310 T11302 punct .,contains
R7656 T11312 T11313 punct (,J
R7657 T11313 T11314 meta J,testis
R7658 T11315 T11313 punct ),J
R7659 T11316 T11314 nmod Mei1,testis
R7660 T11317 T11316 punct −,Mei1
R7661 T11318 T11316 punct /,Mei1
R7662 T11319 T11316 punct −,Mei1
R7663 T11320 T11314 nmod Trip13Gt,testis
R7664 T11321 T11320 punct /,Trip13Gt
R7665 T11322 T11320 punct +,Trip13Gt
R7666 T11323 T11314 punct .,testis
R7667 T11325 T11326 det The,specimen
R7668 T11326 T11327 nsubjpass specimen,taken
R7669 T11328 T11327 auxpass was,taken
R7670 T11329 T11327 prep from,taken
R7671 T11330 T11331 det a,littermate
R7672 T11331 T11329 pobj littermate,from
R7673 T11332 T11331 prep of,littermate
R7674 T11333 T11332 pobj that,of
R7675 T11334 T11333 prep in,that
R7676 T11335 T11336 punct (,K
R7677 T11336 T11334 pobj K,in
R7678 T11337 T11336 punct ),K
R7679 T11338 T11327 punct .,taken
R7680 T11340 T11341 punct (,K
R7681 T11341 T11342 meta K,testis
R7682 T11343 T11341 punct ),K
R7683 T11344 T11342 nmod Mei1,testis
R7684 T11345 T11344 punct −,Mei1
R7685 T11346 T11344 punct /,Mei1
R7686 T11347 T11344 punct −,Mei1
R7687 T11348 T11349 compound Trip13Gt,Gt
R7688 T11349 T11342 compound Gt,testis
R7689 T11350 T11349 punct /,Gt
R7690 T11351 T11342 punct .,testis
R7691 T11353 T11354 punct (,L
R7692 T11354 T11355 meta L,testis
R7693 T11356 T11354 punct ),L
R7694 T11357 T11358 nmod Rec8Mei8,Rec8Mei8
R7695 T11358 T11355 nmod Rec8Mei8,testis
R7696 T11359 T11358 punct /,Rec8Mei8
R7697 T11360 T11355 nmod Trip13Gt,testis
R7698 T11361 T11360 punct /,Trip13Gt
R7699 T11362 T11360 punct +,Trip13Gt
R7700 T11363 T11355 punct .,testis
R7701 T11365 T11366 det The,allele
R7702 T11366 T11368 nsubjpass allele,described
R7703 T11367 T11366 compound Rec8Mei8,allele
R7704 T11369 T11368 auxpass was,described
R7705 T11370 T11371 punct [,39
R7706 T11371 T11368 parataxis 39,described
R7707 T11372 T11371 punct ],39
R7708 T11373 T11368 punct .,described
R7709 T11375 T11376 det The,specimen
R7710 T11376 T11377 nsubjpass specimen,taken
R7711 T11378 T11377 auxpass was,taken
R7712 T11379 T11377 prep from,taken
R7713 T11380 T11381 det a,littermate
R7714 T11381 T11379 pobj littermate,from
R7715 T11382 T11381 prep of,littermate
R7716 T11383 T11382 pobj that,of
R7717 T11384 T11383 prep in,that
R7718 T11385 T11384 punct (,in
R7719 T11386 T11384 pobj M,in
R7720 T11387 T11384 punct ),in
R7721 T11388 T11377 punct .,taken
R7722 T11390 T11391 punct (,M
R7723 T11391 T11392 meta M,testis
R7724 T11393 T11391 punct ),M
R7725 T11394 T11395 compound Rec8Mei8,Rec8Mei8
R7726 T11395 T11392 compound Rec8Mei8,testis
R7727 T11396 T11395 punct /,Rec8Mei8
R7728 T11397 T11398 compound Trip13Gt,Gt
R7729 T11398 T11392 compound Gt,testis
R7730 T11399 T11398 punct /,Gt
R7731 T11400 T11392 punct .,testis
R7732 T11402 T11403 punct (,N
R7733 T11403 T11404 meta N,testis
R7734 T11405 T11403 punct ),N
R7735 T11406 T11404 nmod Dmc1,testis
R7736 T11407 T11406 punct −,Dmc1
R7737 T11408 T11406 punct /,Dmc1
R7738 T11409 T11406 punct −,Dmc1
R7739 T11410 T11411 compound Trip13Gt,Gt
R7740 T11411 T11404 compound Gt,testis
R7741 T11412 T11411 punct /,Gt
R7742 T11413 T11404 punct .,testis
R7743 T11415 T11416 punct (,O
R7744 T11416 T11417 meta O,ovary
R7745 T11418 T11416 punct ),O
R7746 T11419 T11417 nmod Spo11,ovary
R7747 T11420 T11419 punct −,Spo11
R7748 T11421 T11419 punct /,Spo11
R7749 T11422 T11419 punct −,Spo11
R7750 T11423 T11417 nmod Trip13Gt,ovary
R7751 T11424 T11423 punct /,Trip13Gt
R7752 T11425 T11423 punct +,Trip13Gt
R7753 T11426 T11427 nummod 25,d
R7754 T11427 T11429 npadvmod d,old
R7755 T11428 T11427 punct -,d
R7756 T11429 T11417 amod old,ovary
R7757 T11430 T11429 punct -,old
R7758 T11431 T11417 punct .,ovary
R7759 T11433 T11434 det The,specimen
R7760 T11434 T11435 nsubjpass specimen,taken
R7761 T11436 T11435 auxpass was,taken
R7762 T11437 T11435 prep from,taken
R7763 T11438 T11439 det a,littermate
R7764 T11439 T11437 pobj littermate,from
R7765 T11440 T11439 prep of,littermate
R7766 T11441 T11440 pobj that,of
R7767 T11442 T11441 prep in,that
R7768 T11443 T11444 punct (,P
R7769 T11444 T11442 pobj P,in
R7770 T11445 T11444 punct ),P
R7771 T11446 T11435 punct .,taken
R7772 T11448 T11449 punct (,P
R7773 T11449 T11450 meta P,ovary
R7774 T11451 T11449 punct ),P
R7775 T11452 T11450 nmod Spo11,ovary
R7776 T11453 T11452 punct −,Spo11
R7777 T11454 T11452 punct /,Spo11
R7778 T11455 T11452 punct −,Spo11
R7779 T11456 T11457 nmod Trip13Gt,Gt
R7780 T11457 T11450 nmod Gt,ovary
R7781 T11458 T11457 punct /,Gt
R7782 T11459 T11460 nummod 25,d
R7783 T11460 T11462 npadvmod d,old
R7784 T11461 T11460 punct -,d
R7785 T11462 T11450 amod old,ovary
R7786 T11463 T11462 punct -,old
R7787 T11464 T11450 punct .,ovary
R7788 T11466 T11467 punct (,Q
R7789 T11467 T11468 meta Q,ovary
R7790 T11469 T11467 punct ),Q
R7791 T11470 T11468 nmod Mei1,ovary
R7792 T11471 T11470 punct −,Mei1
R7793 T11472 T11470 punct /,Mei1
R7794 T11473 T11470 punct −,Mei1
R7795 T11474 T11468 nmod Trip13Gt,ovary
R7796 T11475 T11474 punct /,Trip13Gt
R7797 T11476 T11474 punct +,Trip13Gt
R7798 T11477 T11478 nummod 25,d
R7799 T11478 T11480 npadvmod d,old
R7800 T11479 T11478 punct -,d
R7801 T11480 T11468 amod old,ovary
R7802 T11481 T11480 punct -,old
R7803 T11482 T11468 punct .,ovary
R7804 T11484 T11485 det The,specimen
R7805 T11485 T11486 nsubjpass specimen,taken
R7806 T11487 T11486 auxpass was,taken
R7807 T11488 T11486 prep from,taken
R7808 T11489 T11490 det a,littermate
R7809 T11490 T11488 pobj littermate,from
R7810 T11491 T11490 prep of,littermate
R7811 T11492 T11491 pobj that,of
R7812 T11493 T11492 prep in,that
R7813 T11494 T11495 punct (,R
R7814 T11495 T11493 pobj R,in
R7815 T11496 T11495 punct ),R
R7816 T11497 T11486 punct .,taken
R7817 T11499 T11500 punct (,R
R7818 T11500 T11501 meta R,ovary
R7819 T11502 T11500 punct ),R
R7820 T11503 T11501 nmod Mei1,ovary
R7821 T11504 T11503 punct −,Mei1
R7822 T11505 T11503 punct /,Mei1
R7823 T11506 T11503 punct −,Mei1
R7824 T11507 T11508 nmod Trip13Gt,Gt
R7825 T11508 T11501 nmod Gt,ovary
R7826 T11509 T11508 punct /,Gt
R7827 T11510 T11511 nummod 25,d
R7828 T11511 T11513 npadvmod d,old
R7829 T11512 T11511 punct -,d
R7830 T11513 T11501 amod old,ovary
R7831 T11514 T11513 punct -,old
R7832 T11515 T11501 punct .,ovary
R7833 T11517 T11518 punct (,S
R7834 T11518 T11519 meta S,ovary
R7835 T11520 T11518 punct ),S
R7836 T11521 T11522 nmod Rec8Mei8,Rec8Mei8
R7837 T11522 T11519 nmod Rec8Mei8,ovary
R7838 T11523 T11522 punct /,Rec8Mei8
R7839 T11524 T11519 nmod Trip13Gt,ovary
R7840 T11525 T11524 punct /,Trip13Gt
R7841 T11526 T11524 punct +,Trip13Gt
R7842 T11527 T11528 nummod 25,d
R7843 T11528 T11530 npadvmod d,old
R7844 T11529 T11528 punct -,d
R7845 T11530 T11519 amod old,ovary
R7846 T11531 T11530 punct -,old
R7847 T11532 T11519 punct .,ovary
R7848 T11534 T11535 det The,specimen
R7849 T11535 T11536 nsubjpass specimen,taken
R7850 T11537 T11536 auxpass was,taken
R7851 T11538 T11536 prep from,taken
R7852 T11539 T11540 det a,littermate
R7853 T11540 T11538 pobj littermate,from
R7854 T11541 T11540 prep of,littermate
R7855 T11542 T11541 pobj that,of
R7856 T11543 T11542 prep in,that
R7857 T11544 T11545 punct (,T
R7858 T11545 T11543 pobj T,in
R7859 T11546 T11545 punct ),T
R7860 T11547 T11536 punct .,taken
R7861 T11549 T11550 punct (,T
R7862 T11550 T11551 meta T,ovary
R7863 T11552 T11550 punct ),T
R7864 T11553 T11554 nmod Rec8Mei8,Rec8Mei8
R7865 T11554 T11551 nmod Rec8Mei8,ovary
R7866 T11555 T11554 punct /,Rec8Mei8
R7867 T11556 T11557 nmod Trip13Gt,Gt
R7868 T11557 T11551 nmod Gt,ovary
R7869 T11558 T11557 punct /,Gt
R7870 T11559 T11560 nummod 25,d
R7871 T11560 T11562 npadvmod d,old
R7872 T11561 T11560 punct -,d
R7873 T11562 T11551 amod old,ovary
R7874 T11563 T11562 punct -,old
R7875 T11564 T11551 punct .,ovary
R7876 T11701 T11702 amod Immunohistochemical,Analysis
R7877 T11703 T11702 prep of,Analysis
R7878 T11704 T11705 compound Pachytene,Chromosomes
R7879 T11705 T11703 pobj Chromosomes,of
R7880 T11706 T11705 compound Spermatocyte,Chromosomes
R7881 T11708 T11709 compound Surface,spread
R7882 T11709 T11711 compound spread,chromosomes
R7883 T11710 T11709 punct -,spread
R7884 T11711 T11712 nsubjpass chromosomes,immunolabeled
R7885 T11713 T11712 auxpass were,immunolabeled
R7886 T11714 T11712 prep with,immunolabeled
R7887 T11715 T11716 det the,antibodies
R7888 T11716 T11714 pobj antibodies,with
R7889 T11717 T11716 amod indicated,antibodies
R7890 T11718 T11716 cc and,antibodies
R7891 T11719 T11716 conj fluorophores,antibodies
R7892 T11720 T11712 punct .,immunolabeled
R7893 T11722 T11723 mark As,indicated
R7894 T11723 T11724 advcl indicated,were
R7895 T11725 T11723 prep in,indicated
R7896 T11726 T11727 det the,right
R7897 T11727 T11725 pobj right,in
R7898 T11728 T11727 amod upper,right
R7899 T11729 T11727 prep of,right
R7900 T11730 T11731 det each,panel
R7901 T11731 T11729 pobj panel,of
R7902 T11732 T11724 punct ", ",were
R7903 T11733 T11724 nsubj cells,were
R7904 T11734 T11724 prep from,were
R7905 T11735 T11736 amod wild,type
R7906 T11736 T11734 pobj type,from
R7907 T11737 T11736 punct (,type
R7908 T11738 T11736 appos WT,type
R7909 T11739 T11738 punct ", ",WT
R7910 T11740 T11741 preconj either,+
R7911 T11741 T11738 appos +,WT
R7912 T11742 T11741 punct +,+
R7913 T11743 T11741 punct /,+
R7914 T11744 T11741 cc or,+
R7915 T11745 T11741 conj Trip13Gt,+
R7916 T11746 T11745 punct /,Trip13Gt
R7917 T11747 T11745 punct +,Trip13Gt
R7918 T11748 T11736 punct ),type
R7919 T11749 T11736 cc or,type
R7920 T11750 T11751 compound Trip13Gt,Gt
R7921 T11751 T11736 conj Gt,type
R7922 T11752 T11751 punct /,Gt
R7923 T11753 T11751 punct (,Gt
R7924 T11754 T11751 appos Mut,Gt
R7925 T11755 T11724 punct ),were
R7926 T11756 T11724 punct .,were
R7927 T11758 T11759 expl There,were
R7928 T11760 T11761 det no,differences
R7929 T11761 T11759 attr differences,were
R7930 T11762 T11761 acl seen,differences
R7931 T11763 T11762 prep between,seen
R7932 T11764 T11763 pobj heterozygotes,between
R7933 T11765 T11764 cc and,heterozygotes
R7934 T11766 T11767 punct +,+
R7935 T11767 T11769 punct +,spermatocytes
R7936 T11768 T11767 punct /,+
R7937 T11769 T11764 conj spermatocytes,heterozygotes
R7938 T11770 T11759 punct .,were
R7939 T11772 T11773 punct (,A
R7940 T11773 T11774 meta A,nucleus
R7941 T11775 T11773 punct ),A
R7942 T11776 T11774 det A,nucleus
R7943 T11777 T11774 compound mutant,nucleus
R7944 T11778 T11774 compound pachytene,nucleus
R7945 T11779 T11774 prep with,nucleus
R7946 T11780 T11781 amod full,synapsis
R7947 T11781 T11779 pobj synapsis,with
R7948 T11782 T11774 punct .,nucleus
R7949 T11784 T11785 nsubj Areas,are
R7950 T11786 T11784 prep of,Areas
R7951 T11787 T11788 compound SYCP1,SYCP3
R7952 T11788 T11790 compound SYCP3,colabeling
R7953 T11789 T11788 punct /,SYCP3
R7954 T11790 T11786 pobj colabeling,of
R7955 T11791 T11785 acomp yellow,are
R7956 T11792 T11785 punct .,are
R7957 T11794 T11795 punct (,B
R7958 T11795 T11796 meta B,nucleus
R7959 T11797 T11795 punct –,B
R7960 T11798 T11795 dep E,B
R7961 T11799 T11795 punct ),B
R7962 T11800 T11796 compound Spermatocytes,nucleus
R7963 T11801 T11796 prep from,nucleus
R7964 T11802 T11803 nummod 17.5,d
R7965 T11803 T11804 npadvmod d,postpartum
R7966 T11804 T11805 amod postpartum,mutant
R7967 T11805 T11801 pobj mutant,from
R7968 T11806 T11796 punct .,nucleus
R7969 T11808 T11809 amod Asynapsed,chromosomes
R7970 T11809 T11810 nsubjpass chromosomes,indicated
R7971 T11811 T11809 cc or,chromosomes
R7972 T11812 T11809 conj regions,chromosomes
R7973 T11813 T11812 prep of,regions
R7974 T11814 T11813 pobj chromosomes,of
R7975 T11815 T11810 auxpass are,indicated
R7976 T11816 T11810 agent by,indicated
R7977 T11817 T11818 amod white,arrows
R7978 T11818 T11816 pobj arrows,by
R7979 T11819 T11817 cc and,white
R7980 T11820 T11817 conj yellow,white
R7981 T11821 T11810 punct ", ",indicated
R7982 T11822 T11810 advmod respectively,indicated
R7983 T11823 T11810 punct .,indicated
R7984 T11825 T11826 prep Unlike,are
R7985 T11827 T11828 det the,distribution
R7986 T11828 T11825 pobj distribution,Unlike
R7987 T11829 T11828 amod normal,distribution
R7988 T11830 T11828 prep in,distribution
R7989 T11831 T11832 amod wild,type
R7990 T11832 T11834 compound type,spermatocytes
R7991 T11833 T11832 punct -,type
R7992 T11834 T11830 pobj spermatocytes,in
R7993 T11835 T11834 compound pachytene,spermatocytes
R7994 T11836 T11837 punct (,C
R7995 T11837 T11828 parataxis C,distribution
R7996 T11838 T11837 punct ),C
R7997 T11839 T11826 punct ", ",are
R7998 T11840 T11841 compound BLM,foci
R7999 T11841 T11826 nsubj foci,are
R8000 T11842 T11826 acomp present,are
R8001 T11843 T11826 prep on,are
R8002 T11844 T11845 amod synapsed,chromosomes
R8003 T11845 T11843 pobj chromosomes,on
R8004 T11846 T11845 compound pachytene,chromosomes
R8005 T11847 T11845 prep in,chromosomes
R8006 T11848 T11849 det the,mutant
R8007 T11849 T11847 pobj mutant,in
R8008 T11850 T11851 punct (,D
R8009 T11851 T11826 parataxis D,are
R8010 T11852 T11851 punct ),D
R8011 T11853 T11826 punct .,are
R8012 T11855 T11856 compound RAD51,foci
R8013 T11856 T11857 nsubj foci,disappear
R8014 T11858 T11856 punct ", ",foci
R8015 T11859 T11860 dep which,are
R8016 T11860 T11856 relcl are,foci
R8017 T11861 T11860 acomp abundant,are
R8018 T11862 T11863 advmod earlier,in
R8019 T11863 T11860 prep in,are
R8020 T11864 T11863 pobj prophase,in
R8021 T11865 T11857 punct ", ",disappear
R8022 T11866 T11857 prep from,disappear
R8023 T11867 T11866 pobj autosomes,from
R8024 T11868 T11857 prep in,disappear
R8025 T11869 T11870 amod wild,type
R8026 T11870 T11872 compound type,nuclei
R8027 T11871 T11870 punct -,type
R8028 T11872 T11868 pobj nuclei,in
R8029 T11873 T11872 compound pachytene,nuclei
R8030 T11874 T11875 punct (,E
R8031 T11875 T11857 parataxis E,disappear
R8032 T11876 T11875 punct ),E
R8033 T11877 T11857 cc and,disappear
R8034 T11878 T11879 det the,bulk
R8035 T11879 T11880 nsubj bulk,is
R8036 T11880 T11857 conj is,disappear
R8037 T11881 T11879 prep of,bulk
R8038 T11882 T11881 pobj staining,of
R8039 T11883 T11880 prep over,is
R8040 T11884 T11885 det the,body
R8041 T11885 T11883 pobj body,over
R8042 T11886 T11885 compound XY,body
R8043 T11887 T11888 punct (,arrow
R8044 T11888 T11880 parataxis arrow,is
R8045 T11889 T11888 punct ),arrow
R8046 T11890 T11880 punct .,is
R8047 T11892 T11893 punct (,F
R8048 T11893 T11894 meta F,persists
R8049 T11895 T11893 punct ),F
R8050 T11896 T11894 nsubj RAD51,persists
R8051 T11897 T11894 prep on,persists
R8052 T11898 T11899 det the,chromosomes
R8053 T11899 T11897 pobj chromosomes,on
R8054 T11900 T11899 amod synapsed,chromosomes
R8055 T11901 T11899 compound mutant,chromosomes
R8056 T11902 T11903 punct (,arrows
R8057 T11903 T11894 parataxis arrows,persists
R8058 T11904 T11903 punct ),arrows
R8059 T11905 T11894 punct .,persists
R8060 T11907 T11908 punct (,G
R8061 T11908 T11909 meta G,restricted
R8062 T11910 T11908 punct ),G
R8063 T11911 T11912 compound H2AX,phosphorylation
R8064 T11912 T11909 nsubjpass phosphorylation,restricted
R8065 T11913 T11909 auxpass is,restricted
R8066 T11914 T11909 prep to,restricted
R8067 T11915 T11916 det the,body
R8068 T11916 T11914 pobj body,to
R8069 T11917 T11916 compound XY,body
R8070 T11918 T11909 prep in,restricted
R8071 T11919 T11918 pobj WT,in
R8072 T11920 T11909 punct .,restricted
R8073 T11922 T11923 punct (,H
R8074 T11923 T11924 meta H,is
R8075 T11925 T11923 punct ),H
R8076 T11926 T11924 prep In,is
R8077 T11927 T11926 pobj addition,In
R8078 T11928 T11927 prep to,addition
R8079 T11929 T11930 det a,area
R8080 T11930 T11928 pobj area,to
R8081 T11931 T11930 amod large,area
R8082 T11932 T11930 prep of,area
R8083 T11933 T11934 compound γH2AX,staining
R8084 T11934 T11932 pobj staining,of
R8085 T11935 T11936 punct (,arrow
R8086 T11936 T11930 parataxis arrow,area
R8087 T11937 T11936 punct ),arrow
R8088 T11938 T11930 prep over,area
R8089 T11939 T11940 det the,body
R8090 T11940 T11938 pobj body,over
R8091 T11941 T11940 compound XY,body
R8092 T11942 T11924 punct ", ",is
R8093 T11943 T11924 expl there,is
R8094 T11944 T11945 amod extensive,phosphorylation
R8095 T11945 T11924 attr phosphorylation,is
R8096 T11946 T11945 amod autosomal,phosphorylation
R8097 T11947 T11945 compound H2AX,phosphorylation
R8098 T11948 T11949 punct (,arrows
R8099 T11949 T11924 parataxis arrows,is
R8100 T11950 T11949 punct ),arrows
R8101 T11951 T11924 punct .,is
R8102 T11953 T11954 punct (,I
R8103 T11954 T11955 meta I,Note
R8104 T11956 T11954 punct ", ",I
R8105 T11957 T11954 dep J,I
R8106 T11958 T11954 punct ),I
R8107 T11959 T11960 mark that,is
R8108 T11960 T11955 ccomp is,Note
R8109 T11961 T11960 prep in,is
R8110 T11962 T11963 amod wild,type
R8111 T11963 T11965 compound type,spermatocytes
R8112 T11964 T11963 punct -,type
R8113 T11965 T11961 pobj spermatocytes,in
R8114 T11966 T11965 compound pachytene,spermatocytes
R8115 T11967 T11960 punct ", ",is
R8116 T11968 T11960 nsubj TOPBP1,is
R8117 T11969 T11960 acomp present,is
R8118 T11970 T11971 advmod only,over
R8119 T11971 T11960 prep over,is
R8120 T11972 T11973 det the,body
R8121 T11973 T11971 pobj body,over
R8122 T11974 T11973 compound XY,body
R8123 T11975 T11976 punct (,arrow
R8124 T11976 T11960 parataxis arrow,is
R8125 T11977 T11976 amod yellow,arrow
R8126 T11978 T11976 punct ),arrow
R8127 T11979 T11955 punct .,Note
R8128 T11981 T11982 prep In,denotes
R8129 T11983 T11984 det the,mutant
R8130 T11984 T11981 pobj mutant,In
R8131 T11985 T11986 punct (,J
R8132 T11986 T11984 parataxis J,mutant
R8133 T11987 T11986 punct ),J
R8134 T11988 T11982 punct ", ",denotes
R8135 T11989 T11990 det an,arrow
R8136 T11990 T11982 nsubj arrow,denotes
R8137 T11991 T11992 nummod one,area
R8138 T11992 T11982 dobj area,denotes
R8139 T11993 T11992 prep of,area
R8140 T11994 T11995 amod intensive,staining
R8141 T11995 T11993 pobj staining,of
R8142 T11996 T11997 dep that,be
R8143 T11997 T11992 relcl be,area
R8144 T11998 T11997 aux may,be
R8145 T11999 T11997 prep over,be
R8146 T12000 T12001 det the,chromosomes
R8147 T12001 T11999 pobj chromosomes,over
R8148 T12002 T12001 compound sex,chromosomes
R8149 T12003 T11982 punct ", ",denotes
R8150 T12004 T11982 cc but,denotes
R8151 T12005 T12006 amod many,cores
R8152 T12006 T12009 nsubjpass cores,stained
R8153 T12007 T12006 amod other,cores
R8154 T12008 T12006 compound chromosome,cores
R8155 T12009 T11982 conj stained,denotes
R8156 T12010 T12009 auxpass are,stained
R8157 T12011 T12009 advmod positively,stained
R8158 T12012 T12009 punct .,stained
R8159 T12014 T12015 punct (,K
R8160 T12015 T12016 meta K,persists
R8161 T12017 T12015 punct ", ",K
R8162 T12018 T12015 dep L,K
R8163 T12019 T12015 punct ),K
R8164 T12020 T12016 nsubj RPA,persists
R8165 T12021 T12016 prep along,persists
R8166 T12022 T12023 amod synapsed,cores
R8167 T12023 T12021 pobj cores,along
R8168 T12024 T12016 prep in,persists
R8169 T12025 T12026 det the,mutant
R8170 T12026 T12024 pobj mutant,in
R8171 T12027 T12026 punct ", ",mutant
R8172 T12028 T12026 cc not,mutant
R8173 T12029 T12026 conj WT,mutant
R8174 T12030 T12016 punct .,persists
R8175 T12032 T12033 punct (,M
R8176 T12033 T12034 meta M,indicate
R8177 T12035 T12033 punct ", ",M
R8178 T12036 T12033 dep N,M
R8179 T12037 T12033 punct ),M
R8180 T12038 T12034 nsubj Arrows,indicate
R8181 T12039 T12034 dobj examples,indicate
R8182 T12040 T12039 prep of,examples
R8183 T12041 T12042 compound MLH3,foci
R8184 T12042 T12040 pobj foci,of
R8185 T12043 T12042 prep on,foci
R8186 T12044 T12043 pobj SCs,on
R8187 T12045 T12034 punct .,indicate
R8188 T12047 T12048 punct (,O
R8189 T12048 T12049 meta O,is
R8190 T12050 T12048 punct ),O
R8191 T12051 T12049 prep In,is
R8192 T12052 T12053 nmod WT,spermatocytes
R8193 T12053 T12051 pobj spermatocytes,In
R8194 T12054 T12053 amod late,spermatocytes
R8195 T12055 T12053 compound pachytene,spermatocytes
R8196 T12056 T12049 punct ", ",is
R8197 T12057 T12049 nsubj RAD51,is
R8198 T12058 T12049 acomp present,is
R8199 T12059 T12060 advmod only,at
R8200 T12060 T12049 prep at,is
R8201 T12061 T12062 compound background,levels
R8202 T12062 T12060 pobj levels,at
R8203 T12063 T12049 punct .,is
R8204 T12065 T12066 punct (,P
R8205 T12066 T12067 meta P,delineates
R8206 T12068 T12066 punct ),P
R8207 T12069 T12067 prep As,delineates
R8208 T12070 T12069 prep in,As
R8209 T12071 T12072 punct (,F
R8210 T12072 T12070 pobj F,in
R8211 T12073 T12072 punct ),F
R8212 T12074 T12067 punct ", ",delineates
R8213 T12075 T12076 amod extensive,staining
R8214 T12076 T12067 nsubj staining,delineates
R8215 T12077 T12076 compound RAD51,staining
R8216 T12078 T12067 dobj SCs,delineates
R8217 T12079 T12078 prep in,SCs
R8218 T12080 T12081 compound mutant,nuclei
R8219 T12081 T12079 pobj nuclei,in
R8220 T12082 T12081 compound pachytene,nuclei
R8221 T12083 T12078 punct (,SCs
R8222 T12084 T12078 acl indicated,SCs
R8223 T12085 T12084 agent by,indicated
R8224 T12086 T12087 amod white,arcs
R8225 T12087 T12085 pobj arcs,by
R8226 T12088 T12067 punct ),delineates
R8227 T12089 T12067 punct .,delineates
R8228 T12091 T12092 compound MLH1,foci
R8229 T12092 T12093 nsubj foci,colocalize
R8230 T12094 T12093 prep with,colocalize
R8231 T12095 T12096 det these,tracts
R8232 T12096 T12094 pobj tracts,with
R8233 T12097 T12098 punct (,arrows
R8234 T12098 T12096 parataxis arrows,tracts
R8235 T12099 T12098 punct ),arrows
R8236 T12100 T12093 prep at,colocalize
R8237 T12101 T12102 det the,foci
R8238 T12102 T12100 pobj foci,at
R8239 T12103 T12102 amod typical,foci
R8240 T12104 T12105 quantmod 1,2
R8241 T12105 T12102 nummod 2,foci
R8242 T12106 T12105 punct –,2
R8243 T12107 T12102 prep per,foci
R8244 T12108 T12107 pobj chromosome,per
R8245 T12109 T12093 prep as,colocalize
R8246 T12110 T12109 prep in,as
R8247 T12111 T12112 punct (,M
R8248 T12112 T12110 pobj M,in
R8249 T12113 T12112 punct ),M
R8250 T12114 T12093 punct .,colocalize
R8251 T12126 T12127 amod Immunocytological,Analysis
R8252 T12128 T12127 prep of,Analysis
R8253 T12129 T12130 compound Trip13,Mutants
R8254 T12130 T12128 pobj Mutants,of
R8255 T12131 T12130 compound Compound,Mutants
R8256 T12133 T12134 compound Surface,spread
R8257 T12134 T12136 compound spread,chromosomes
R8258 T12135 T12134 punct -,spread
R8259 T12136 T12137 nsubjpass chromosomes,immunolabeled
R8260 T12138 T12137 auxpass were,immunolabeled
R8261 T12139 T12137 prep with,immunolabeled
R8262 T12140 T12141 det the,antibodies
R8263 T12141 T12139 pobj antibodies,with
R8264 T12142 T12141 amod indicated,antibodies
R8265 T12143 T12141 cc and,antibodies
R8266 T12144 T12141 conj fluorophores,antibodies
R8267 T12145 T12137 punct .,immunolabeled
R8268 T12147 T12148 nsubjpass Genotypes,indicated
R8269 T12149 T12148 auxpass are,indicated
R8270 T12150 T12148 punct ", ",indicated
R8271 T12151 T12152 mark as,are
R8272 T12152 T12148 advcl are,indicated
R8273 T12153 T12154 det those,panels
R8274 T12154 T12152 nsubj panels,are
R8275 T12155 T12156 prep in,merged
R8276 T12156 T12154 relcl merged,panels
R8277 T12157 T12155 pobj which,in
R8278 T12158 T12159 amod dual,patterns
R8279 T12159 T12156 nsubjpass patterns,merged
R8280 T12160 T12159 compound staining,patterns
R8281 T12161 T12156 auxpass are,merged
R8282 T12162 T12148 punct .,indicated
R8283 T12165 T12166 mark that,are
R8284 T12166 T12164 ccomp are,Note
R8285 T12167 T12168 punct (,H
R8286 T12168 T12166 nsubj H,are
R8287 T12169 T12168 punct ),H
R8288 T12170 T12168 cc and,H
R8289 T12171 T12172 punct (,I
R8290 T12172 T12168 conj I,H
R8291 T12173 T12172 punct ),I
R8292 T12174 T12166 prep at,are
R8293 T12175 T12176 amod lower,magnification
R8294 T12176 T12174 pobj magnification,at
R8295 T12177 T12178 aux to,show
R8296 T12178 T12166 advcl show,are
R8297 T12179 T12180 amod multiple,nuclei
R8298 T12180 T12178 dobj nuclei,show
R8299 T12181 T12164 punct .,Note
R879 T1407 T1408 nsubj Trip13,Is
R880 T1409 T1410 det a,Ortholog
R881 T1410 T1408 attr Ortholog,Is
R882 T1411 T1412 advmod Widely,Expressed
R883 T1412 T1410 amod Expressed,Ortholog
R884 T1413 T1410 amod Mammalian,Ortholog
R885 T1414 T1410 prep of,Ortholog
R886 T1415 T1414 pobj PCH2,of
R887 T1416 T1410 prep with,Ortholog
R888 T1417 T1418 amod Unusual,Relationships
R889 T1418 T1416 pobj Relationships,with
R890 T1419 T1418 amod Phylogenetic,Relationships
R891 T1421 T1422 det The,ortholog
R892 T1422 T1424 nsubj ortholog,encodes
R893 T1423 T1422 amod mammalian,ortholog
R894 T1425 T1422 prep of,ortholog
R895 T1426 T1425 pobj PCH2,of
R896 T1427 T1422 punct ", ",ortholog
R897 T1428 T1422 appos Trip13,ortholog
R898 T1429 T1428 punct (,Trip13
R899 T1430 T1431 compound thyroid,receptor
R900 T1431 T1433 npadvmod receptor,interacting
R901 T1432 T1431 compound hormone,receptor
R902 T1433 T1434 amod interacting,protein
R903 T1434 T1428 appos protein,Trip13
R904 T1435 T1434 nummod 13,protein
R905 T1436 T1424 punct ),encodes
R906 T1437 T1424 punct ", ",encodes
R907 T1438 T1439 det a,protein
R908 T1439 T1424 dobj protein,encodes
R909 T1440 T1439 prep with,protein
R910 T1441 T1442 amod extensive,homology
R911 T1442 T1440 pobj homology,with
R912 T1443 T1444 compound amino,acid
R913 T1444 T1442 compound acid,homology
R914 T1445 T1442 prep in,homology
R915 T1446 T1445 pobj regions,in
R916 T1447 T1446 amod alignable,regions
R917 T1448 T1447 prep to,alignable
R918 T1449 T1450 det the,orthologs
R919 T1450 T1448 pobj orthologs,to
R920 T1451 T1450 nmod yeast,orthologs
R921 T1452 T1451 cc and,yeast
R922 T1453 T1451 conj worm,yeast
R923 T1454 T1455 punct (,S1
R924 T1455 T1424 parataxis S1,encodes
R925 T1456 T1455 compound Figure,S1
R926 T1457 T1455 punct ),S1
R927 T1458 T1459 punct [,12
R928 T1459 T1424 parataxis 12,encodes
R929 T1460 T1459 punct ],12
R930 T1461 T1424 punct ),encodes
R931 T1462 T1424 punct .,encodes
R932 T1464 T1465 advmod Interestingly,shows
R933 T1466 T1465 punct ", ",shows
R934 T1467 T1468 amod phylogenetic,analysis
R935 T1468 T1465 nsubj analysis,shows
R936 T1469 T1468 prep of,analysis
R937 T1470 T1471 compound TRIP13,Pch2p
R938 T1471 T1469 pobj Pch2p,of
R939 T1472 T1471 punct /,Pch2p
R940 T1473 T1474 mark that,clusters
R941 T1474 T1465 ccomp clusters,shows
R942 T1475 T1476 det the,protein
R943 T1476 T1474 nsubj protein,clusters
R944 T1477 T1476 amod mammalian,protein
R945 T1478 T1479 advmod more,closely
R946 T1479 T1474 advmod closely,clusters
R947 T1480 T1474 prep to,clusters
R948 T1481 T1480 pobj plants,to
R949 T1482 T1483 mark than,to
R950 T1483 T1474 advcl to,clusters
R951 T1484 T1483 nsubj it,to
R952 T1485 T1483 aux does,to
R953 T1486 T1487 det the,worms
R954 T1487 T1483 pobj worms,to
R955 T1488 T1489 advmod evolutionarily,related
R956 T1489 T1487 amod related,worms
R957 T1490 T1491 advmod more,closely
R958 T1491 T1489 advmod closely,related
R959 T1492 T1487 cc and,worms
R960 T1493 T1487 conj flies,worms
R961 T1494 T1495 punct (,1A
R962 T1495 T1465 parataxis 1A,shows
R963 T1496 T1495 compound Figure,1A
R964 T1497 T1495 punct ;,1A
R965 T1498 T1495 advcl see,1A
R966 T1499 T1498 dobj Discussion,see
R967 T1500 T1495 punct ),1A
R968 T1501 T1465 punct .,shows
R969 T1503 T1504 amod Semi-quantitative,analysis
R970 T1504 T1514 nsubj analysis,showed
R971 T1505 T1506 nmod reverse,transcriptase
R972 T1506 T1508 nmod transcriptase,PCR
R973 T1507 T1506 punct -,transcriptase
R974 T1508 T1504 nmod PCR,analysis
R975 T1509 T1508 punct (,PCR
R976 T1510 T1511 compound RT,PCR
R977 T1511 T1508 appos PCR,PCR
R978 T1512 T1511 punct -,PCR
R979 T1513 T1504 punct ),analysis
R980 T1515 T1516 compound Trip13,mRNA
R981 T1516 T1517 nsubjpass mRNA,expressed
R982 T1517 T1514 ccomp expressed,showed
R983 T1518 T1517 aux to,expressed
R984 T1519 T1517 auxpass be,expressed
R985 T1520 T1517 prep in,expressed
R986 T1521 T1522 det a,variety
R987 T1522 T1520 pobj variety,in
R988 T1523 T1522 prep of,variety
R989 T1524 T1525 amod embryonic,tissues
R990 T1525 T1523 pobj tissues,of
R991 T1526 T1524 cc and,embryonic
R992 T1527 T1524 conj adult,embryonic
R993 T1528 T1525 punct ", ",tissues
R994 T1529 T1525 prep including,tissues
R995 T1530 T1529 pobj testis,including
R996 T1531 T1532 punct (,1B
R997 T1532 T1530 parataxis 1B,testis
R998 T1533 T1532 compound Figure,1B
R999 T1534 T1532 punct ),1B