PMC:1941754 / 43315-45265 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T9322 0-19 NN denotes Immunocytochemistry
T9323 19-20 . denotes .
T9324 20-115 sentence denotes Immunolabeling of surface-spread spermatocytes and oocytes was performed as described [39,62].
T9325 21-35 NN denotes Immunolabeling
T9327 36-38 IN denotes of
T9328 39-46 NN denotes surface
T9330 46-47 HYPH denotes -
T9331 47-53 NN denotes spread
T9329 54-67 NNS denotes spermatocytes
T9332 68-71 CC denotes and
T9333 72-79 NNS denotes oocytes
T9334 80-83 VBD denotes was
T9326 84-93 VBN denotes performed
T9335 94-96 IN denotes as
T9336 97-106 VBN denotes described
T9337 107-108 -LRB- denotes [
T9339 108-110 CD denotes 39
T9340 110-111 , denotes ,
T9338 111-113 CD denotes 62
T9341 113-114 -RRB- denotes ]
T9342 114-115 . denotes .
T9343 115-491 sentence denotes To reach conclusions on the pattern of staining for various proteins, 30 (unless otherwise indicated) well-spread nuclei of particular meiotic stages were first identified under the fluorescent microscope on the basis of SYCP3 or STAG3 staining, then imaged at both appropriate wavelengths to determine the pattern of second proteins with focal patterns such as RAD51 or RPA.
T9344 116-118 TO denotes To
T9345 119-124 VB denotes reach
T9347 125-136 NNS denotes conclusions
T9348 137-139 IN denotes on
T9349 140-143 DT denotes the
T9350 144-151 NN denotes pattern
T9351 152-154 IN denotes of
T9352 155-163 NN denotes staining
T9353 164-167 IN denotes for
T9354 168-175 JJ denotes various
T9355 176-184 NN denotes proteins
T9356 184-186 , denotes ,
T9357 186-188 CD denotes 30
T9359 189-190 -LRB- denotes (
T9361 190-196 IN denotes unless
T9362 197-206 RB denotes otherwise
T9360 207-216 VBN denotes indicated
T9363 216-217 -RRB- denotes )
T9364 218-222 RB denotes well
T9366 222-223 HYPH denotes -
T9365 223-229 VBN denotes spread
T9358 230-236 NNS denotes nuclei
T9367 237-239 IN denotes of
T9368 240-250 JJ denotes particular
T9370 251-258 JJ denotes meiotic
T9369 259-265 NNS denotes stages
T9371 266-270 VBD denotes were
T9372 271-276 RB denotes first
T9346 277-287 VBN denotes identified
T9373 288-293 IN denotes under
T9374 294-297 DT denotes the
T9376 298-309 JJ denotes fluorescent
T9375 310-320 NN denotes microscope
T9377 321-323 IN denotes on
T9378 324-327 DT denotes the
T9379 328-333 NN denotes basis
T9380 334-336 IN denotes of
T9381 337-342 NN denotes SYCP3
T9383 343-345 CC denotes or
T9384 346-351 NN denotes STAG3
T9382 352-360 NN denotes staining
T9385 360-362 , denotes ,
T9386 362-366 RB denotes then
T9387 367-373 VBN denotes imaged
T9388 374-376 IN denotes at
T9389 377-381 DT denotes both
T9391 382-393 JJ denotes appropriate
T9390 394-405 NNS denotes wavelengths
T9392 406-408 TO denotes to
T9393 409-418 VB denotes determine
T9394 419-422 DT denotes the
T9395 423-430 NN denotes pattern
T9396 431-433 IN denotes of
T9397 434-440 JJ denotes second
T9398 441-449 NN denotes proteins
T9399 450-454 IN denotes with
T9400 455-460 JJ denotes focal
T9401 461-469 NNS denotes patterns
T9402 470-474 JJ denotes such
T9403 475-477 IN denotes as
T9404 478-483 NN denotes RAD51
T9405 484-486 CC denotes or
T9406 487-490 NN denotes RPA
T9407 490-491 . denotes .
T9408 491-600 sentence denotes Unless otherwise indicated, the panels shown in the figures were the exclusive or predominant patterns seen.
T9409 492-498 IN denotes Unless
T9411 499-508 RB denotes otherwise
T9410 509-518 VBN denotes indicated
T9413 518-520 , denotes ,
T9414 520-523 DT denotes the
T9415 524-530 NNS denotes panels
T9416 531-536 VBN denotes shown
T9417 537-539 IN denotes in
T9418 540-543 DT denotes the
T9419 544-551 NNS denotes figures
T9412 552-556 VBD denotes were
T9420 557-560 DT denotes the
T9422 561-570 JJ denotes exclusive
T9423 571-573 CC denotes or
T9424 574-585 JJ denotes predominant
T9421 586-594 NNS denotes patterns
T9425 595-599 VBN denotes seen
T9426 599-600 . denotes .
T9427 600-763 sentence denotes The exception for this approach was in the case of staining for MLH1 or MLH3 plus RAD51 (in which case SYCP3 or STAG3 was not available to find chromosome cores).
T9428 601-604 DT denotes The
T9429 605-614 NN denotes exception
T9431 615-618 IN denotes for
T9432 619-623 DT denotes this
T9433 624-632 NN denotes approach
T9430 633-636 VBD denotes was
T9434 637-639 IN denotes in
T9435 640-643 DT denotes the
T9436 644-648 NN denotes case
T9437 649-651 IN denotes of
T9438 652-660 VBG denotes staining
T9439 661-664 IN denotes for
T9440 665-669 NN denotes MLH1
T9441 670-672 CC denotes or
T9442 673-677 NN denotes MLH3
T9443 678-682 CC denotes plus
T9444 683-688 NN denotes RAD51
T9445 689-690 -LRB- denotes (
T9446 690-692 IN denotes in
T9448 693-698 WDT denotes which
T9449 699-703 NN denotes case
T9450 704-709 NN denotes SYCP3
T9451 710-712 CC denotes or
T9452 713-718 NN denotes STAG3
T9447 719-722 VBD denotes was
T9453 723-726 RB denotes not
T9454 727-736 JJ denotes available
T9455 737-739 TO denotes to
T9456 740-744 VB denotes find
T9457 745-755 NN denotes chromosome
T9458 756-761 NNS denotes cores
T9459 761-762 -RRB- denotes )
T9460 762-763 . denotes .
T9461 763-883 sentence denotes Nuclei in this situation were identified first by MLH1/3 foci clustering, then imaged for both fluorescent wavelengths.
T9462 764-770 NNS denotes Nuclei
T9464 771-773 IN denotes in
T9465 774-778 DT denotes this
T9466 779-788 NN denotes situation
T9467 789-793 VBD denotes were
T9463 794-804 VBN denotes identified
T9468 805-810 RB denotes first
T9469 811-813 IN denotes by
T9470 814-818 NN denotes MLH1
T9472 818-819 HYPH denotes /
T9473 819-820 CD denotes 3
T9471 821-825 NNS denotes foci
T9474 826-836 VBG denotes clustering
T9475 836-838 , denotes ,
T9476 838-842 RB denotes then
T9477 843-849 VBN denotes imaged
T9478 850-853 IN denotes for
T9479 854-858 DT denotes both
T9481 859-870 JJ denotes fluorescent
T9480 871-882 NNS denotes wavelengths
T9482 882-883 . denotes .
T9483 883-1723 sentence denotes Primary antibodies used in this study were as follows: mouse anti-SCP3 (1:500; Abcam, http://www.abcam.com); rabbit anti-SYCP1 (1:1,000; a gift from C. Heyting) [63]; rabbit anti-REC8 (1:100; a gift from C. Heyting); rabbit anti-RAD51 (1:250, this polyclonal antibody recognizes both RAD51 and DMC1; Oncogene Research Products, http://www.merckbiosciences.co.uk); rabbit anti-γH2AX (1:500; Upstate Biotechnology, http://www.upstate.com/); rabbit anti-STAG3 (1:1,000; a gift from R. Jessberger); rabbit anti-MLH3 (1:400; a gift from P. Cohen); mouse-anti-human MLH1 (1:50; BD Biosciences, http://www.bdbiosciences.com); rabbit-anti-TopBP1 (1:100; a gift from J. Chen) [22]; mouse-anti-ubiquityl-histone H2A (1:200; Upstate Biotechnology); rabbit-anti-TRF2 (1:500; a gift from T. de Lange); and rabbit-anti-BLM (1:50; a gift from R. Freire).
T9484 884-891 JJ denotes Primary
T9485 892-902 NNS denotes antibodies
T9487 903-907 VBN denotes used
T9488 908-910 IN denotes in
T9489 911-915 DT denotes this
T9490 916-921 NN denotes study
T9486 922-926 VBD denotes were
T9491 927-929 IN denotes as
T9492 930-937 VBZ denotes follows
T9493 937-939 : denotes :
T9494 939-944 NN denotes mouse
T9495 945-954 JJ denotes anti-SCP3
T9496 955-956 -LRB- denotes (
T9498 956-957 CD denotes 1
T9499 957-958 SYM denotes :
T9500 958-961 CD denotes 500
T9501 961-962 : denotes ;
T9497 963-968 NNP denotes Abcam
T9502 968-970 , denotes ,
T9503 970-990 NN denotes http://www.abcam.com
T9504 990-991 -RRB- denotes )
T9505 991-992 : denotes ;
T9506 993-999 NN denotes rabbit
T9507 1000-1010 NN denotes anti-SYCP1
T9508 1011-1012 -LRB- denotes (
T9510 1012-1013 CD denotes 1
T9511 1013-1014 SYM denotes :
T9512 1014-1019 CD denotes 1,000
T9513 1019-1020 : denotes ;
T9514 1021-1022 DT denotes a
T9509 1023-1027 NN denotes gift
T9515 1028-1032 IN denotes from
T9516 1033-1035 NNP denotes C.
T9517 1036-1043 NNP denotes Heyting
T9518 1043-1044 -RRB- denotes )
T9519 1045-1046 -LRB- denotes [
T9520 1046-1048 CD denotes 63
T9521 1048-1049 -RRB- denotes ]
T9522 1049-1050 : denotes ;
T9523 1051-1057 NN denotes rabbit
T9524 1058-1067 NN denotes anti-REC8
T9525 1068-1069 -LRB- denotes (
T9527 1069-1070 CD denotes 1
T9528 1070-1071 SYM denotes :
T9529 1071-1074 CD denotes 100
T9530 1074-1075 : denotes ;
T9531 1076-1077 DT denotes a
T9526 1078-1082 NN denotes gift
T9532 1083-1087 IN denotes from
T9533 1088-1090 NNP denotes C.
T9534 1091-1098 NNP denotes Heyting
T9535 1098-1099 -RRB- denotes )
T9536 1099-1100 : denotes ;
T9537 1101-1107 NN denotes rabbit
T9538 1108-1118 NN denotes anti-RAD51
T9539 1119-1120 -LRB- denotes (
T9541 1120-1121 CD denotes 1
T9542 1121-1122 SYM denotes :
T9543 1122-1125 CD denotes 250
T9544 1125-1127 , denotes ,
T9545 1127-1131 DT denotes this
T9547 1132-1142 JJ denotes polyclonal
T9546 1143-1151 NN denotes antibody
T9548 1152-1162 VBZ denotes recognizes
T9549 1163-1167 CC denotes both
T9550 1168-1173 NN denotes RAD51
T9551 1174-1177 CC denotes and
T9552 1178-1182 NN denotes DMC1
T9553 1182-1183 : denotes ;
T9554 1184-1192 NNP denotes Oncogene
T9555 1193-1201 NNP denotes Research
T9540 1202-1210 NNP denotes Products
T9556 1210-1212 , denotes ,
T9557 1212-1245 NN denotes http://www.merckbiosciences.co.uk
T9558 1245-1246 -RRB- denotes )
T9559 1246-1247 : denotes ;
T9560 1248-1254 NN denotes rabbit
T9561 1255-1265 NN denotes anti-γH2AX
T9562 1266-1267 -LRB- denotes (
T9564 1267-1268 CD denotes 1
T9565 1268-1269 SYM denotes :
T9566 1269-1272 CD denotes 500
T9567 1272-1273 : denotes ;
T9568 1274-1281 NNP denotes Upstate
T9563 1282-1295 NNP denotes Biotechnology
T9569 1295-1297 , denotes ,
T9570 1297-1320 NN denotes http://www.upstate.com/
T9571 1320-1321 -RRB- denotes )
T9572 1321-1322 : denotes ;
T9573 1323-1329 NN denotes rabbit
T9574 1330-1340 NN denotes anti-STAG3
T9575 1341-1342 -LRB- denotes (
T9577 1342-1343 CD denotes 1
T9578 1343-1344 SYM denotes :
T9579 1344-1349 CD denotes 1,000
T9580 1349-1350 : denotes ;
T9581 1351-1352 DT denotes a
T9576 1353-1357 NN denotes gift
T9582 1358-1362 IN denotes from
T9583 1363-1365 NNP denotes R.
T9584 1366-1376 NNP denotes Jessberger
T9585 1376-1377 -RRB- denotes )
T9586 1377-1378 : denotes ;
T9587 1379-1385 NN denotes rabbit
T9588 1386-1395 NN denotes anti-MLH3
T9589 1396-1397 -LRB- denotes (
T9591 1397-1398 CD denotes 1
T9592 1398-1399 SYM denotes :
T9593 1399-1402 CD denotes 400
T9594 1402-1403 : denotes ;
T9595 1404-1405 DT denotes a
T9590 1406-1410 NN denotes gift
T9596 1411-1415 IN denotes from
T9597 1416-1418 NNP denotes P.
T9598 1419-1424 NNP denotes Cohen
T9599 1424-1425 -RRB- denotes )
T9600 1425-1426 : denotes ;
T9601 1427-1432 JJ denotes mouse
T9603 1432-1433 HYPH denotes -
T9604 1433-1443 JJ denotes anti-human
T9602 1444-1448 NN denotes MLH1
T9605 1449-1450 -LRB- denotes (
T9607 1450-1451 CD denotes 1
T9608 1451-1452 SYM denotes :
T9609 1452-1454 CD denotes 50
T9610 1454-1455 : denotes ;
T9611 1456-1458 NNP denotes BD
T9606 1459-1470 NNP denotes Biosciences
T9612 1470-1472 , denotes ,
T9613 1472-1500 NN denotes http://www.bdbiosciences.com
T9614 1500-1501 -RRB- denotes )
T9615 1501-1502 : denotes ;
T9616 1503-1509 NN denotes rabbit
T9618 1509-1510 HYPH denotes -
T9617 1510-1521 NN denotes anti-TopBP1
T9619 1522-1523 -LRB- denotes (
T9621 1523-1524 CD denotes 1
T9622 1524-1525 SYM denotes :
T9623 1525-1528 CD denotes 100
T9624 1528-1529 : denotes ;
T9625 1530-1531 DT denotes a
T9620 1532-1536 NN denotes gift
T9626 1537-1541 IN denotes from
T9627 1542-1544 NNP denotes J.
T9628 1545-1549 NNP denotes Chen
T9629 1549-1550 -RRB- denotes )
T9630 1551-1552 -LRB- denotes [
T9631 1552-1554 CD denotes 22
T9632 1554-1555 -RRB- denotes ]
T9633 1555-1556 : denotes ;
T9634 1557-1562 NN denotes mouse
T9636 1562-1563 HYPH denotes -
T9637 1563-1577 JJ denotes anti-ubiquityl
T9638 1577-1578 HYPH denotes -
T9639 1578-1585 NN denotes histone
T9635 1586-1589 NN denotes H2A
T9640 1590-1591 -LRB- denotes (
T9642 1591-1592 CD denotes 1
T9643 1592-1593 SYM denotes :
T9644 1593-1596 CD denotes 200
T9645 1596-1597 : denotes ;
T9646 1598-1605 NNP denotes Upstate
T9641 1606-1619 NNP denotes Biotechnology
T9647 1619-1620 -RRB- denotes )
T9648 1620-1621 : denotes ;
T9649 1622-1628 NN denotes rabbit
T9651 1628-1629 HYPH denotes -
T9650 1629-1638 NN denotes anti-TRF2
T9652 1639-1640 -LRB- denotes (
T9654 1640-1641 CD denotes 1
T9655 1641-1642 SYM denotes :
T9656 1642-1645 CD denotes 500
T9657 1645-1646 : denotes ;
T9658 1647-1648 DT denotes a
T9653 1649-1653 NN denotes gift
T9659 1654-1658 IN denotes from
T9660 1659-1661 NNP denotes T.
T9662 1662-1664 NNP denotes de
T9661 1665-1670 NNP denotes Lange
T9663 1670-1671 -RRB- denotes )
T9664 1671-1672 : denotes ;
T9665 1673-1676 CC denotes and
T9666 1677-1683 NN denotes rabbit
T9668 1683-1684 HYPH denotes -
T9667 1684-1692 NN denotes anti-BLM
T9669 1693-1694 -LRB- denotes (
T9671 1694-1695 CD denotes 1
T9672 1695-1696 SYM denotes :
T9673 1696-1698 CD denotes 50
T9674 1698-1699 : denotes ;
T9675 1700-1701 DT denotes a
T9670 1702-1706 NN denotes gift
T9676 1707-1711 IN denotes from
T9677 1712-1714 NNP denotes R.
T9678 1715-1721 NNP denotes Freire
T9679 1721-1722 -RRB- denotes )
T9680 1722-1723 . denotes .
T9681 1723-1880 sentence denotes All secondary antibodies conjugated with either Alexa Fluor 488 or 594 (Molecular Probes, http://probes.invitrogen.com/) were used at a dilution of 1:1,000.
T9682 1724-1727 DT denotes All
T9684 1728-1737 JJ denotes secondary
T9683 1738-1748 NNS denotes antibodies
T9686 1749-1759 VBN denotes conjugated
T9687 1760-1764 IN denotes with
T9688 1765-1771 CC denotes either
T9690 1772-1777 NNP denotes Alexa
T9691 1778-1783 NNP denotes Fluor
T9689 1784-1787 CD denotes 488
T9692 1788-1790 CC denotes or
T9693 1791-1794 CD denotes 594
T9694 1795-1796 -LRB- denotes (
T9696 1796-1805 NNP denotes Molecular
T9695 1806-1812 NNP denotes Probes
T9697 1812-1814 , denotes ,
T9698 1814-1843 NN denotes http://probes.invitrogen.com/
T9699 1843-1844 -RRB- denotes )
T9700 1845-1849 VBD denotes were
T9685 1850-1854 VBN denotes used
T9701 1855-1857 IN denotes at
T9702 1858-1859 DT denotes a
T9703 1860-1868 NN denotes dilution
T9704 1869-1871 IN denotes of
T9705 1872-1873 CD denotes 1
T9706 1873-1874 SYM denotes :
T9707 1874-1879 CD denotes 1,000
T9708 1879-1880 . denotes .
T9709 1880-1950 sentence denotes All images were taken with a 100× objective lens under immersion oil.
T9710 1881-1884 DT denotes All
T9711 1885-1891 NNS denotes images
T9713 1892-1896 VBD denotes were
T9712 1897-1902 VBN denotes taken
T9714 1903-1907 IN denotes with
T9715 1908-1909 DT denotes a
T9717 1910-1913 CD denotes 100
T9718 1913-1914 SYM denotes ×
T9719 1915-1924 NN denotes objective
T9716 1925-1929 NN denotes lens
T9720 1930-1935 IN denotes under
T9721 1936-1945 NN denotes immersion
T9722 1946-1949 NN denotes oil
T9723 1949-1950 . denotes .
R6260 T9323 T9322 punct .,Immunocytochemistry
R6261 T9325 T9326 nsubjpass Immunolabeling,performed
R6262 T9327 T9325 prep of,Immunolabeling
R6263 T9328 T9329 compound surface,spermatocytes
R6264 T9329 T9327 pobj spermatocytes,of
R6265 T9330 T9329 punct -,spermatocytes
R6266 T9331 T9329 compound spread,spermatocytes
R6267 T9332 T9329 cc and,spermatocytes
R6268 T9333 T9329 conj oocytes,spermatocytes
R6269 T9334 T9326 auxpass was,performed
R6270 T9335 T9336 mark as,described
R6271 T9336 T9326 advcl described,performed
R6272 T9337 T9338 punct [,62
R6273 T9338 T9326 parataxis 62,performed
R6274 T9339 T9338 nummod 39,62
R6275 T9340 T9338 punct ",",62
R6276 T9341 T9338 punct ],62
R6277 T9342 T9326 punct .,performed
R6278 T9344 T9345 aux To,reach
R6279 T9345 T9346 advcl reach,identified
R6280 T9347 T9345 dobj conclusions,reach
R6281 T9348 T9347 prep on,conclusions
R6282 T9349 T9350 det the,pattern
R6283 T9350 T9348 pobj pattern,on
R6284 T9351 T9350 prep of,pattern
R6285 T9352 T9351 pobj staining,of
R6286 T9353 T9350 prep for,pattern
R6287 T9354 T9355 amod various,proteins
R6288 T9355 T9353 pobj proteins,for
R6289 T9356 T9346 punct ", ",identified
R6290 T9357 T9358 nummod 30,nuclei
R6291 T9358 T9346 nsubjpass nuclei,identified
R6292 T9359 T9360 punct (,indicated
R6293 T9360 T9358 parataxis indicated,nuclei
R6294 T9361 T9360 mark unless,indicated
R6295 T9362 T9360 advmod otherwise,indicated
R6296 T9363 T9360 punct ),indicated
R6297 T9364 T9365 advmod well,spread
R6298 T9365 T9358 amod spread,nuclei
R6299 T9366 T9365 punct -,spread
R6300 T9367 T9358 prep of,nuclei
R6301 T9368 T9369 amod particular,stages
R6302 T9369 T9367 pobj stages,of
R6303 T9370 T9369 amod meiotic,stages
R6304 T9371 T9346 auxpass were,identified
R6305 T9372 T9346 advmod first,identified
R6306 T9373 T9346 prep under,identified
R6307 T9374 T9375 det the,microscope
R6308 T9375 T9373 pobj microscope,under
R6309 T9376 T9375 amod fluorescent,microscope
R6310 T9377 T9346 prep on,identified
R6311 T9378 T9379 det the,basis
R6312 T9379 T9377 pobj basis,on
R6313 T9380 T9379 prep of,basis
R6314 T9381 T9382 nmod SYCP3,staining
R6315 T9382 T9380 pobj staining,of
R6316 T9383 T9381 cc or,SYCP3
R6317 T9384 T9381 conj STAG3,SYCP3
R6318 T9385 T9346 punct ", ",identified
R6319 T9386 T9387 advmod then,imaged
R6320 T9387 T9346 dep imaged,identified
R6321 T9388 T9387 prep at,imaged
R6322 T9389 T9390 det both,wavelengths
R6323 T9390 T9388 pobj wavelengths,at
R6324 T9391 T9390 amod appropriate,wavelengths
R6325 T9392 T9393 aux to,determine
R6326 T9393 T9387 advcl determine,imaged
R6327 T9394 T9395 det the,pattern
R6328 T9395 T9393 dobj pattern,determine
R6329 T9396 T9395 prep of,pattern
R6330 T9397 T9398 amod second,proteins
R6331 T9398 T9396 pobj proteins,of
R6332 T9399 T9393 prep with,determine
R6333 T9400 T9401 amod focal,patterns
R6334 T9401 T9399 pobj patterns,with
R6335 T9402 T9403 amod such,as
R6336 T9403 T9401 prep as,patterns
R6337 T9404 T9403 pobj RAD51,as
R6338 T9405 T9404 cc or,RAD51
R6339 T9406 T9404 conj RPA,RAD51
R6340 T9407 T9346 punct .,identified
R6341 T9409 T9410 mark Unless,indicated
R6342 T9410 T9412 advcl indicated,were
R6343 T9411 T9410 advmod otherwise,indicated
R6344 T9413 T9412 punct ", ",were
R6345 T9414 T9415 det the,panels
R6346 T9415 T9412 nsubj panels,were
R6347 T9416 T9415 acl shown,panels
R6348 T9417 T9416 prep in,shown
R6349 T9418 T9419 det the,figures
R6350 T9419 T9417 pobj figures,in
R6351 T9420 T9421 det the,patterns
R6352 T9421 T9412 attr patterns,were
R6353 T9422 T9421 amod exclusive,patterns
R6354 T9423 T9422 cc or,exclusive
R6355 T9424 T9422 conj predominant,exclusive
R6356 T9425 T9421 acl seen,patterns
R6357 T9426 T9412 punct .,were
R6358 T9428 T9429 det The,exception
R6359 T9429 T9430 nsubj exception,was
R6360 T9431 T9429 prep for,exception
R6361 T9432 T9433 det this,approach
R6362 T9433 T9431 pobj approach,for
R6363 T9434 T9430 prep in,was
R6364 T9435 T9436 det the,case
R6365 T9436 T9434 pobj case,in
R6366 T9437 T9436 prep of,case
R6367 T9438 T9437 pcomp staining,of
R6368 T9439 T9438 prep for,staining
R6369 T9440 T9439 pobj MLH1,for
R6370 T9441 T9440 cc or,MLH1
R6371 T9442 T9440 conj MLH3,MLH1
R6372 T9443 T9440 cc plus,MLH1
R6373 T9444 T9440 conj RAD51,MLH1
R6374 T9445 T9444 punct (,RAD51
R6375 T9446 T9447 prep in,was
R6376 T9447 T9444 advcl was,RAD51
R6377 T9448 T9449 det which,case
R6378 T9449 T9446 pcomp case,in
R6379 T9450 T9447 nsubj SYCP3,was
R6380 T9451 T9450 cc or,SYCP3
R6381 T9452 T9450 conj STAG3,SYCP3
R6382 T9453 T9447 neg not,was
R6383 T9454 T9447 acomp available,was
R6384 T9455 T9456 aux to,find
R6385 T9456 T9447 advcl find,was
R6386 T9457 T9458 compound chromosome,cores
R6387 T9458 T9456 dobj cores,find
R6388 T9459 T9430 punct ),was
R6389 T9460 T9430 punct .,was
R6390 T9462 T9463 nsubjpass Nuclei,identified
R6391 T9464 T9462 prep in,Nuclei
R6392 T9465 T9466 det this,situation
R6393 T9466 T9464 pobj situation,in
R6394 T9467 T9463 auxpass were,identified
R6395 T9468 T9463 advmod first,identified
R6396 T9469 T9463 agent by,identified
R6397 T9470 T9471 nmod MLH1,foci
R6398 T9471 T9469 pobj foci,by
R6399 T9472 T9470 punct /,MLH1
R6400 T9473 T9470 nummod 3,MLH1
R6401 T9474 T9471 amod clustering,foci
R6402 T9475 T9463 punct ", ",identified
R6403 T9476 T9477 advmod then,imaged
R6404 T9477 T9463 dep imaged,identified
R6405 T9478 T9477 prep for,imaged
R6406 T9479 T9480 det both,wavelengths
R6407 T9480 T9478 pobj wavelengths,for
R6408 T9481 T9480 amod fluorescent,wavelengths
R6409 T9482 T9463 punct .,identified
R6410 T9484 T9485 amod Primary,antibodies
R6411 T9485 T9486 nsubj antibodies,were
R6412 T9487 T9485 acl used,antibodies
R6413 T9488 T9487 prep in,used
R6414 T9489 T9490 det this,study
R6415 T9490 T9488 pobj study,in
R6416 T9491 T9492 mark as,follows
R6417 T9492 T9486 advcl follows,were
R6418 T9493 T9486 punct : ,were
R6419 T9494 T9486 dep mouse,were
R6420 T9495 T9494 amod anti-SCP3,mouse
R6421 T9496 T9497 punct (,Abcam
R6422 T9497 T9494 parataxis Abcam,mouse
R6423 T9498 T9497 dep 1,Abcam
R6424 T9499 T9500 punct :,500
R6425 T9500 T9498 prep 500,1
R6426 T9501 T9497 punct ;,Abcam
R6427 T9502 T9497 punct ", ",Abcam
R6428 T9503 T9497 npadvmod http://www.abcam.com,Abcam
R6429 T9504 T9497 punct ),Abcam
R6430 T9505 T9494 punct ;,mouse
R6431 T9506 T9507 compound rabbit,anti-SYCP1
R6432 T9507 T9494 conj anti-SYCP1,mouse
R6433 T9508 T9509 punct (,gift
R6434 T9509 T9507 parataxis gift,anti-SYCP1
R6435 T9510 T9509 dep 1,gift
R6436 T9511 T9512 punct :,"1,000"
R6437 T9512 T9510 prep "1,000",1
R6438 T9513 T9509 punct ;,gift
R6439 T9514 T9509 det a,gift
R6440 T9515 T9509 prep from,gift
R6441 T9516 T9517 compound C.,Heyting
R6442 T9517 T9515 pobj Heyting,from
R6443 T9518 T9509 punct ),gift
R6444 T9519 T9520 punct [,63
R6445 T9520 T9507 parataxis 63,anti-SYCP1
R6446 T9521 T9520 punct ],63
R6447 T9522 T9507 punct ;,anti-SYCP1
R6448 T9523 T9524 compound rabbit,anti-REC8
R6449 T9524 T9507 conj anti-REC8,anti-SYCP1
R6450 T9525 T9526 punct (,gift
R6451 T9526 T9524 parataxis gift,anti-REC8
R6452 T9527 T9526 dep 1,gift
R6453 T9528 T9529 punct :,100
R6454 T9529 T9527 prep 100,1
R6455 T9530 T9526 punct ;,gift
R6456 T9531 T9526 det a,gift
R6457 T9532 T9526 prep from,gift
R6458 T9533 T9534 compound C.,Heyting
R6459 T9534 T9532 pobj Heyting,from
R6460 T9535 T9526 punct ),gift
R6461 T9536 T9524 punct ;,anti-REC8
R6462 T9537 T9538 compound rabbit,anti-RAD51
R6463 T9538 T9524 conj anti-RAD51,anti-REC8
R6464 T9539 T9540 punct (,Products
R6465 T9540 T9538 parataxis Products,anti-RAD51
R6466 T9541 T9540 dep 1,Products
R6467 T9542 T9543 punct :,250
R6468 T9543 T9541 prep 250,1
R6469 T9544 T9540 punct ", ",Products
R6470 T9545 T9546 det this,antibody
R6471 T9546 T9548 nsubj antibody,recognizes
R6472 T9547 T9546 amod polyclonal,antibody
R6473 T9548 T9540 ccomp recognizes,Products
R6474 T9549 T9550 preconj both,RAD51
R6475 T9550 T9548 dobj RAD51,recognizes
R6476 T9551 T9550 cc and,RAD51
R6477 T9552 T9550 conj DMC1,RAD51
R6478 T9553 T9540 punct ;,Products
R6479 T9554 T9540 compound Oncogene,Products
R6480 T9555 T9540 compound Research,Products
R6481 T9556 T9540 punct ", ",Products
R6482 T9557 T9540 npadvmod http://www.merckbiosciences.co.uk,Products
R6483 T9558 T9540 punct ),Products
R6484 T9559 T9538 punct ;,anti-RAD51
R6485 T9560 T9561 compound rabbit,anti-γH2AX
R6486 T9561 T9538 conj anti-γH2AX,anti-RAD51
R6487 T9562 T9563 punct (,Biotechnology
R6488 T9563 T9561 parataxis Biotechnology,anti-γH2AX
R6489 T9564 T9563 dep 1,Biotechnology
R6490 T9565 T9566 punct :,500
R6491 T9566 T9564 prep 500,1
R6492 T9567 T9563 punct ;,Biotechnology
R6493 T9568 T9563 compound Upstate,Biotechnology
R6494 T9569 T9563 punct ", ",Biotechnology
R6495 T9570 T9563 npadvmod http://www.upstate.com/,Biotechnology
R6496 T9571 T9563 punct ),Biotechnology
R6497 T9572 T9561 punct ;,anti-γH2AX
R6498 T9573 T9574 compound rabbit,anti-STAG3
R6499 T9574 T9561 conj anti-STAG3,anti-γH2AX
R6500 T9575 T9576 punct (,gift
R6501 T9576 T9574 parataxis gift,anti-STAG3
R6502 T9577 T9576 dep 1,gift
R6503 T9578 T9579 punct :,"1,000"
R6504 T9579 T9577 prep "1,000",1
R6505 T9580 T9576 punct ;,gift
R6506 T9581 T9576 det a,gift
R6507 T9582 T9576 prep from,gift
R6508 T9583 T9584 compound R.,Jessberger
R6509 T9584 T9582 pobj Jessberger,from
R6510 T9585 T9576 punct ),gift
R6511 T9586 T9574 punct ;,anti-STAG3
R6512 T9587 T9588 compound rabbit,anti-MLH3
R6513 T9588 T9574 conj anti-MLH3,anti-STAG3
R6514 T9589 T9590 punct (,gift
R6515 T9590 T9588 parataxis gift,anti-MLH3
R6516 T9591 T9590 dep 1,gift
R6517 T9592 T9593 punct :,400
R6518 T9593 T9591 prep 400,1
R6519 T9594 T9590 punct ;,gift
R6520 T9595 T9590 det a,gift
R6521 T9596 T9590 prep from,gift
R6522 T9597 T9598 compound P.,Cohen
R6523 T9598 T9596 pobj Cohen,from
R6524 T9599 T9590 punct ),gift
R6525 T9600 T9588 punct ;,anti-MLH3
R6526 T9601 T9602 amod mouse,MLH1
R6527 T9602 T9588 conj MLH1,anti-MLH3
R6528 T9603 T9602 punct -,MLH1
R6529 T9604 T9602 amod anti-human,MLH1
R6530 T9605 T9606 punct (,Biosciences
R6531 T9606 T9602 parataxis Biosciences,MLH1
R6532 T9607 T9606 dep 1,Biosciences
R6533 T9608 T9609 punct :,50
R6534 T9609 T9607 prep 50,1
R6535 T9610 T9606 punct ;,Biosciences
R6536 T9611 T9606 compound BD,Biosciences
R6537 T9612 T9606 punct ", ",Biosciences
R6538 T9613 T9606 npadvmod http://www.bdbiosciences.com,Biosciences
R6539 T9614 T9606 punct ),Biosciences
R6540 T9615 T9602 punct ;,MLH1
R6541 T9616 T9617 compound rabbit,anti-TopBP1
R6542 T9617 T9602 conj anti-TopBP1,MLH1
R6543 T9618 T9617 punct -,anti-TopBP1
R6544 T9619 T9620 punct (,gift
R6545 T9620 T9617 parataxis gift,anti-TopBP1
R6546 T9621 T9620 dep 1,gift
R6547 T9622 T9623 punct :,100
R6548 T9623 T9621 prep 100,1
R6549 T9624 T9620 punct ;,gift
R6550 T9625 T9620 det a,gift
R6551 T9626 T9620 prep from,gift
R6552 T9627 T9628 compound J.,Chen
R6553 T9628 T9626 pobj Chen,from
R6554 T9629 T9620 punct ),gift
R6555 T9630 T9631 punct [,22
R6556 T9631 T9617 parataxis 22,anti-TopBP1
R6557 T9632 T9631 punct ],22
R6558 T9633 T9617 punct ;,anti-TopBP1
R6559 T9634 T9635 nmod mouse,H2A
R6560 T9635 T9617 conj H2A,anti-TopBP1
R6561 T9636 T9635 punct -,H2A
R6562 T9637 T9635 amod anti-ubiquityl,H2A
R6563 T9638 T9635 punct -,H2A
R6564 T9639 T9635 compound histone,H2A
R6565 T9640 T9641 punct (,Biotechnology
R6566 T9641 T9635 parataxis Biotechnology,H2A
R6567 T9642 T9641 dep 1,Biotechnology
R6568 T9643 T9644 punct :,200
R6569 T9644 T9642 prep 200,1
R6570 T9645 T9641 punct ;,Biotechnology
R6571 T9646 T9641 compound Upstate,Biotechnology
R6572 T9647 T9641 punct ),Biotechnology
R6573 T9648 T9635 punct ;,H2A
R6574 T9649 T9650 compound rabbit,anti-TRF2
R6575 T9650 T9635 conj anti-TRF2,H2A
R6576 T9651 T9650 punct -,anti-TRF2
R6577 T9652 T9653 punct (,gift
R6578 T9653 T9650 parataxis gift,anti-TRF2
R6579 T9654 T9653 dep 1,gift
R6580 T9655 T9656 punct :,500
R6581 T9656 T9654 prep 500,1
R6582 T9657 T9653 punct ;,gift
R6583 T9658 T9653 det a,gift
R6584 T9659 T9653 prep from,gift
R6585 T9660 T9661 compound T.,Lange
R6586 T9661 T9659 pobj Lange,from
R6587 T9662 T9661 compound de,Lange
R6588 T9663 T9653 punct ),gift
R6589 T9664 T9650 punct ;,anti-TRF2
R6590 T9665 T9650 cc and,anti-TRF2
R6591 T9666 T9667 compound rabbit,anti-BLM
R6592 T9667 T9650 conj anti-BLM,anti-TRF2
R6593 T9668 T9667 punct -,anti-BLM
R6594 T9669 T9670 punct (,gift
R6595 T9670 T9667 parataxis gift,anti-BLM
R6596 T9671 T9670 dep 1,gift
R6597 T9672 T9673 punct :,50
R6598 T9673 T9671 prep 50,1
R6599 T9674 T9670 punct ;,gift
R6600 T9675 T9670 det a,gift
R6601 T9676 T9670 prep from,gift
R6602 T9677 T9678 compound R.,Freire
R6603 T9678 T9676 pobj Freire,from
R6604 T9679 T9670 punct ),gift
R6605 T9680 T9486 punct .,were
R6606 T9682 T9683 det All,antibodies
R6607 T9683 T9685 nsubjpass antibodies,used
R6608 T9684 T9683 amod secondary,antibodies
R6609 T9686 T9683 acl conjugated,antibodies
R6610 T9687 T9686 prep with,conjugated
R6611 T9688 T9689 preconj either,488
R6612 T9689 T9687 pobj 488,with
R6613 T9690 T9689 nmod Alexa,488
R6614 T9691 T9689 nmod Fluor,488
R6615 T9692 T9689 cc or,488
R6616 T9693 T9689 conj 594,488
R6617 T9694 T9695 punct (,Probes
R6618 T9695 T9689 parataxis Probes,488
R6619 T9696 T9695 compound Molecular,Probes
R6620 T9697 T9695 punct ", ",Probes
R6621 T9698 T9695 npadvmod http://probes.invitrogen.com/,Probes
R6622 T9699 T9695 punct ),Probes
R6623 T9700 T9685 auxpass were,used
R6624 T9701 T9685 prep at,used
R6625 T9702 T9703 det a,dilution
R6626 T9703 T9701 pobj dilution,at
R6627 T9704 T9703 prep of,dilution
R6628 T9705 T9704 pobj 1,of
R6629 T9706 T9707 punct :,"1,000"
R6630 T9707 T9705 prep "1,000",1
R6631 T9708 T9685 punct .,used
R6632 T9710 T9711 det All,images
R6633 T9711 T9712 nsubjpass images,taken
R6634 T9713 T9712 auxpass were,taken
R6635 T9714 T9712 prep with,taken
R6636 T9715 T9716 det a,lens
R6637 T9716 T9714 pobj lens,with
R6638 T9717 T9716 nummod 100,lens
R6639 T9718 T9717 punct ×,100
R6640 T9719 T9716 compound objective,lens
R6641 T9720 T9712 prep under,taken
R6642 T9721 T9722 compound immersion,oil
R6643 T9722 T9720 pobj oil,under
R6644 T9723 T9712 punct .,taken