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PMC:1941754 / 42377-42944 JSONTXT 3 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T9024 0-7 NNP denotes Western
T9025 8-16 NN denotes blotting
T9026 16-17 . denotes .
T9027 17-178 sentence denotes 50 μg of testis extract in RIPA buffer was separated by 8% SDS-PAGE and electrotransferred onto a Pure Nitrocellulose membrane (Bio-Rad, http://www.biorad.com).
T9028 18-20 CD denotes 50
T9029 21-23 NN denotes μg
T9031 24-26 IN denotes of
T9032 27-33 NN denotes testis
T9033 34-41 NN denotes extract
T9034 42-44 IN denotes in
T9035 45-49 NN denotes RIPA
T9036 50-56 NN denotes buffer
T9037 57-60 VBD denotes was
T9030 61-70 VBN denotes separated
T9038 71-73 IN denotes by
T9039 74-75 CD denotes 8
T9040 75-76 NN denotes %
T9042 77-80 NN denotes SDS
T9043 80-81 HYPH denotes -
T9041 81-85 NN denotes PAGE
T9044 86-89 CC denotes and
T9045 90-108 VBD denotes electrotransferred
T9046 109-113 IN denotes onto
T9047 114-115 DT denotes a
T9049 116-120 NNP denotes Pure
T9050 121-135 NNP denotes Nitrocellulose
T9048 136-144 NN denotes membrane
T9051 145-146 -LRB- denotes (
T9053 146-149 NNP denotes Bio
T9054 149-150 HYPH denotes -
T9052 150-153 NNP denotes Rad
T9055 153-155 , denotes ,
T9056 155-176 NN denotes http://www.biorad.com
T9057 176-177 -RRB- denotes )
T9058 177-178 . denotes .
T9059 178-307 sentence denotes The membrane was incubated with a polyclonal rabbit anti-human TRIP13 antibody (18-003-42687; Genway, http://www.genwaybio.com).
T9060 179-182 DT denotes The
T9061 183-191 NN denotes membrane
T9063 192-195 VBD denotes was
T9062 196-205 VBN denotes incubated
T9064 206-210 IN denotes with
T9065 211-212 DT denotes a
T9067 213-223 JJ denotes polyclonal
T9068 224-230 NN denotes rabbit
T9069 231-241 JJ denotes anti-human
T9070 242-248 NN denotes TRIP13
T9066 249-257 NN denotes antibody
T9071 258-259 -LRB- denotes (
T9073 259-261 CD denotes 18
T9075 261-262 HYPH denotes -
T9076 262-265 CD denotes 003
T9077 265-266 HYPH denotes -
T9074 266-271 CD denotes 42687
T9078 271-272 : denotes ;
T9072 273-279 NNP denotes Genway
T9079 279-281 , denotes ,
T9080 281-305 NN denotes http://www.genwaybio.com
T9081 305-306 -RRB- denotes )
T9082 306-307 . denotes .
T9083 307-433 sentence denotes According to the manufacturer, the immunogen was a synthetic peptide embedded in sequence we deduced to correspond to exon 3.
T9084 308-317 VBG denotes According
T9086 318-320 IN denotes to
T9087 321-324 DT denotes the
T9088 325-337 NN denotes manufacturer
T9089 337-339 , denotes ,
T9090 339-342 DT denotes the
T9091 343-352 NN denotes immunogen
T9085 353-356 VBD denotes was
T9092 357-358 DT denotes a
T9094 359-368 JJ denotes synthetic
T9093 369-376 NN denotes peptide
T9095 377-385 VBN denotes embedded
T9096 386-388 IN denotes in
T9097 389-397 NN denotes sequence
T9098 398-400 PRP denotes we
T9099 401-408 VBD denotes deduced
T9100 409-411 TO denotes to
T9101 412-422 VB denotes correspond
T9102 423-425 IN denotes to
T9103 426-430 NN denotes exon
T9104 431-432 CD denotes 3
T9105 432-433 . denotes .
T9106 433-567 sentence denotes Binding was detected by chemiluminescence ECL kit (Pierce) using a rabbit anti-chicken IgG horseradish peroxidase conjugate (Pierce).
T9107 434-441 NN denotes Binding
T9109 442-445 VBD denotes was
T9108 446-454 VBN denotes detected
T9110 455-457 IN denotes by
T9111 458-475 NN denotes chemiluminescence
T9113 476-479 NN denotes ECL
T9112 480-483 NN denotes kit
T9114 484-485 -LRB- denotes (
T9115 485-491 NNP denotes Pierce
T9116 491-492 -RRB- denotes )
T9117 493-498 VBG denotes using
T9118 499-500 DT denotes a
T9120 501-507 NN denotes rabbit
T9121 508-520 JJ denotes anti-chicken
T9122 521-524 NN denotes IgG
T9123 525-536 NN denotes horseradish
T9124 537-547 NN denotes peroxidase
T9119 548-557 NN denotes conjugate
T9125 558-559 -LRB- denotes (
T9126 559-565 NNP denotes Pierce
T9127 565-566 -RRB- denotes )
T9128 566-567 . denotes .
R6100 T9024 T9025 compound Western,blotting
R6101 T9026 T9025 punct .,blotting
R6102 T9028 T9029 nummod 50,μg
R6103 T9029 T9030 nsubjpass μg,separated
R6104 T9031 T9029 prep of,μg
R6105 T9032 T9033 compound testis,extract
R6106 T9033 T9031 pobj extract,of
R6107 T9034 T9029 prep in,μg
R6108 T9035 T9036 compound RIPA,buffer
R6109 T9036 T9034 pobj buffer,in
R6110 T9037 T9030 auxpass was,separated
R6111 T9038 T9030 agent by,separated
R6112 T9039 T9040 nummod 8,%
R6113 T9040 T9041 compound %,PAGE
R6114 T9041 T9038 pobj PAGE,by
R6115 T9042 T9041 compound SDS,PAGE
R6116 T9043 T9041 punct -,PAGE
R6117 T9044 T9030 cc and,separated
R6118 T9045 T9030 conj electrotransferred,separated
R6119 T9046 T9045 prep onto,electrotransferred
R6120 T9047 T9048 det a,membrane
R6121 T9048 T9046 pobj membrane,onto
R6122 T9049 T9048 compound Pure,membrane
R6123 T9050 T9048 compound Nitrocellulose,membrane
R6124 T9051 T9052 punct (,Rad
R6125 T9052 T9048 parataxis Rad,membrane
R6126 T9053 T9052 compound Bio,Rad
R6127 T9054 T9052 punct -,Rad
R6128 T9055 T9052 punct ", ",Rad
R6129 T9056 T9052 npadvmod http://www.biorad.com,Rad
R6130 T9057 T9052 punct ),Rad
R6131 T9058 T9030 punct .,separated
R6132 T9060 T9061 det The,membrane
R6133 T9061 T9062 nsubjpass membrane,incubated
R6134 T9063 T9062 auxpass was,incubated
R6135 T9064 T9062 prep with,incubated
R6136 T9065 T9066 det a,antibody
R6137 T9066 T9064 pobj antibody,with
R6138 T9067 T9068 amod polyclonal,rabbit
R6139 T9068 T9066 nmod rabbit,antibody
R6140 T9069 T9066 amod anti-human,antibody
R6141 T9070 T9066 compound TRIP13,antibody
R6142 T9071 T9072 punct (,Genway
R6143 T9072 T9066 parataxis Genway,antibody
R6144 T9073 T9074 nummod 18,42687
R6145 T9074 T9072 dep 42687,Genway
R6146 T9075 T9074 punct -,42687
R6147 T9076 T9074 nummod 003,42687
R6148 T9077 T9074 punct -,42687
R6149 T9078 T9072 punct ;,Genway
R6150 T9079 T9072 punct ", ",Genway
R6151 T9080 T9072 npadvmod http://www.genwaybio.com,Genway
R6152 T9081 T9072 punct ),Genway
R6153 T9082 T9062 punct .,incubated
R6154 T9084 T9085 prep According,was
R6155 T9086 T9084 prep to,According
R6156 T9087 T9088 det the,manufacturer
R6157 T9088 T9086 pobj manufacturer,to
R6158 T9089 T9085 punct ", ",was
R6159 T9090 T9091 det the,immunogen
R6160 T9091 T9085 nsubj immunogen,was
R6161 T9092 T9093 det a,peptide
R6162 T9093 T9085 attr peptide,was
R6163 T9094 T9093 amod synthetic,peptide
R6164 T9095 T9093 acl embedded,peptide
R6165 T9096 T9095 prep in,embedded
R6166 T9097 T9096 pobj sequence,in
R6167 T9098 T9099 nsubj we,deduced
R6168 T9099 T9097 advcl deduced,sequence
R6169 T9100 T9101 aux to,correspond
R6170 T9101 T9099 xcomp correspond,deduced
R6171 T9102 T9101 prep to,correspond
R6172 T9103 T9102 pobj exon,to
R6173 T9104 T9103 nummod 3,exon
R6174 T9105 T9085 punct .,was
R6175 T9107 T9108 nsubjpass Binding,detected
R6176 T9109 T9108 auxpass was,detected
R6177 T9110 T9108 prep by,detected
R6178 T9111 T9112 compound chemiluminescence,kit
R6179 T9112 T9110 pobj kit,by
R6180 T9113 T9112 compound ECL,kit
R6181 T9114 T9115 punct (,Pierce
R6182 T9115 T9112 parataxis Pierce,kit
R6183 T9116 T9115 punct ),Pierce
R6184 T9117 T9108 advcl using,detected
R6185 T9118 T9119 det a,conjugate
R6186 T9119 T9117 dobj conjugate,using
R6187 T9120 T9119 nmod rabbit,conjugate
R6188 T9121 T9119 amod anti-chicken,conjugate
R6189 T9122 T9119 compound IgG,conjugate
R6190 T9123 T9124 compound horseradish,peroxidase
R6191 T9124 T9119 compound peroxidase,conjugate
R6192 T9125 T9126 punct (,Pierce
R6193 T9126 T9119 parataxis Pierce,conjugate
R6194 T9127 T9126 punct ),Pierce
R6195 T9128 T9108 punct .,detected