Id |
Subject |
Object |
Predicate |
Lexical cue |
T8918 |
0-11 |
NN |
denotes |
Development |
T8919 |
12-15 |
CC |
denotes |
and |
T8920 |
16-28 |
NN |
denotes |
purification |
T8921 |
29-31 |
IN |
denotes |
of |
T8922 |
32-39 |
NN |
denotes |
chicken |
T8923 |
40-50 |
NNS |
denotes |
antibodies |
T8924 |
50-51 |
. |
denotes |
. |
T8925 |
51-230 |
sentence |
denotes |
A peptide corresponding to amino acids 25–40 of TRIP13, VLQRSGSTAKKEDIK, was conjugated to KLH and used to immunize chickens (done by Sigma Genosys, http://www.sigmaaldrich.com). |
T8926 |
52-53 |
DT |
denotes |
A |
T8927 |
54-61 |
NN |
denotes |
peptide |
T8929 |
62-75 |
VBG |
denotes |
corresponding |
T8930 |
76-78 |
IN |
denotes |
to |
T8931 |
79-84 |
NN |
denotes |
amino |
T8933 |
85-90 |
NNS |
denotes |
acids |
T8932 |
91-93 |
CD |
denotes |
25 |
T8934 |
93-94 |
SYM |
denotes |
– |
T8935 |
94-96 |
CD |
denotes |
40 |
T8936 |
97-99 |
IN |
denotes |
of |
T8937 |
100-106 |
NN |
denotes |
TRIP13 |
T8938 |
106-108 |
, |
denotes |
, |
T8939 |
108-123 |
NN |
denotes |
VLQRSGSTAKKEDIK |
T8940 |
123-125 |
, |
denotes |
, |
T8941 |
125-128 |
VBD |
denotes |
was |
T8928 |
129-139 |
VBN |
denotes |
conjugated |
T8942 |
140-142 |
IN |
denotes |
to |
T8943 |
143-146 |
NN |
denotes |
KLH |
T8944 |
147-150 |
CC |
denotes |
and |
T8945 |
151-155 |
VBN |
denotes |
used |
T8946 |
156-158 |
TO |
denotes |
to |
T8947 |
159-167 |
VB |
denotes |
immunize |
T8948 |
168-176 |
NNS |
denotes |
chickens |
T8949 |
177-178 |
-LRB- |
denotes |
( |
T8950 |
178-182 |
VBN |
denotes |
done |
T8951 |
183-185 |
IN |
denotes |
by |
T8952 |
186-191 |
NNP |
denotes |
Sigma |
T8953 |
192-199 |
NNP |
denotes |
Genosys |
T8954 |
199-201 |
, |
denotes |
, |
T8955 |
201-228 |
NN |
denotes |
http://www.sigmaaldrich.com |
T8956 |
228-229 |
-RRB- |
denotes |
) |
T8957 |
229-230 |
. |
denotes |
. |
T8958 |
230-353 |
sentence |
denotes |
Polyclonal IgY was isolated from eggs with the Eggcellent Chicken IgY Purification kit (Pierce, http://www.piercenet.com). |
T8959 |
231-241 |
JJ |
denotes |
Polyclonal |
T8960 |
242-245 |
NN |
denotes |
IgY |
T8962 |
246-249 |
VBD |
denotes |
was |
T8961 |
250-258 |
VBN |
denotes |
isolated |
T8963 |
259-263 |
IN |
denotes |
from |
T8964 |
264-268 |
NNS |
denotes |
eggs |
T8965 |
269-273 |
IN |
denotes |
with |
T8966 |
274-277 |
DT |
denotes |
the |
T8968 |
278-288 |
NNP |
denotes |
Eggcellent |
T8969 |
289-296 |
NNP |
denotes |
Chicken |
T8970 |
297-300 |
NNP |
denotes |
IgY |
T8971 |
301-313 |
NNP |
denotes |
Purification |
T8967 |
314-317 |
NN |
denotes |
kit |
T8972 |
318-319 |
-LRB- |
denotes |
( |
T8973 |
319-325 |
NNP |
denotes |
Pierce |
T8974 |
325-327 |
, |
denotes |
, |
T8975 |
327-351 |
NN |
denotes |
http://www.piercenet.com |
T8976 |
351-352 |
-RRB- |
denotes |
) |
T8977 |
352-353 |
. |
denotes |
. |
T8978 |
353-436 |
sentence |
denotes |
IgY antibodies were then affinity purified using the immunizing synthetic peptide. |
T8979 |
354-357 |
NN |
denotes |
IgY |
T8980 |
358-368 |
NNS |
denotes |
antibodies |
T8982 |
369-373 |
VBD |
denotes |
were |
T8983 |
374-378 |
RB |
denotes |
then |
T8984 |
379-387 |
NN |
denotes |
affinity |
T8981 |
388-396 |
VBN |
denotes |
purified |
T8985 |
397-402 |
VBG |
denotes |
using |
T8986 |
403-406 |
DT |
denotes |
the |
T8988 |
407-417 |
VBG |
denotes |
immunizing |
T8989 |
418-427 |
JJ |
denotes |
synthetic |
T8987 |
428-435 |
NN |
denotes |
peptide |
T8990 |
435-436 |
. |
denotes |
. |
R6034 |
T8919 |
T8918 |
cc |
and,Development |
R6035 |
T8920 |
T8918 |
conj |
purification,Development |
R6036 |
T8921 |
T8918 |
prep |
of,Development |
R6037 |
T8922 |
T8923 |
compound |
chicken,antibodies |
R6038 |
T8923 |
T8921 |
pobj |
antibodies,of |
R6039 |
T8924 |
T8918 |
punct |
.,Development |
R6040 |
T8926 |
T8927 |
det |
A,peptide |
R6041 |
T8927 |
T8928 |
nsubjpass |
peptide,conjugated |
R6042 |
T8929 |
T8927 |
acl |
corresponding,peptide |
R6043 |
T8930 |
T8929 |
prep |
to,corresponding |
R6044 |
T8931 |
T8932 |
nmod |
amino,25 |
R6045 |
T8932 |
T8930 |
pobj |
25,to |
R6046 |
T8933 |
T8932 |
nmod |
acids,25 |
R6047 |
T8934 |
T8935 |
punct |
–,40 |
R6048 |
T8935 |
T8932 |
prep |
40,25 |
R6049 |
T8936 |
T8932 |
prep |
of,25 |
R6050 |
T8937 |
T8936 |
pobj |
TRIP13,of |
R6051 |
T8938 |
T8927 |
punct |
", ",peptide |
R6052 |
T8939 |
T8927 |
appos |
VLQRSGSTAKKEDIK,peptide |
R6053 |
T8940 |
T8928 |
punct |
", ",conjugated |
R6054 |
T8941 |
T8928 |
auxpass |
was,conjugated |
R6055 |
T8942 |
T8928 |
prep |
to,conjugated |
R6056 |
T8943 |
T8942 |
pobj |
KLH,to |
R6057 |
T8944 |
T8928 |
cc |
and,conjugated |
R6058 |
T8945 |
T8928 |
conj |
used,conjugated |
R6059 |
T8946 |
T8947 |
aux |
to,immunize |
R6060 |
T8947 |
T8945 |
advcl |
immunize,used |
R6061 |
T8948 |
T8947 |
dobj |
chickens,immunize |
R6062 |
T8949 |
T8950 |
punct |
(,done |
R6063 |
T8950 |
T8945 |
parataxis |
done,used |
R6064 |
T8951 |
T8950 |
prep |
by,done |
R6065 |
T8952 |
T8953 |
compound |
Sigma,Genosys |
R6066 |
T8953 |
T8951 |
pobj |
Genosys,by |
R6067 |
T8954 |
T8953 |
punct |
", ",Genosys |
R6068 |
T8955 |
T8953 |
npadvmod |
http://www.sigmaaldrich.com,Genosys |
R6069 |
T8956 |
T8950 |
punct |
),done |
R6070 |
T8957 |
T8928 |
punct |
.,conjugated |
R6071 |
T8959 |
T8960 |
amod |
Polyclonal,IgY |
R6072 |
T8960 |
T8961 |
nsubjpass |
IgY,isolated |
R6073 |
T8962 |
T8961 |
auxpass |
was,isolated |
R6074 |
T8963 |
T8961 |
prep |
from,isolated |
R6075 |
T8964 |
T8963 |
pobj |
eggs,from |
R6076 |
T8965 |
T8961 |
prep |
with,isolated |
R6077 |
T8966 |
T8967 |
det |
the,kit |
R6078 |
T8967 |
T8965 |
pobj |
kit,with |
R6079 |
T8968 |
T8969 |
compound |
Eggcellent,Chicken |
R6080 |
T8969 |
T8967 |
compound |
Chicken,kit |
R6081 |
T8970 |
T8967 |
compound |
IgY,kit |
R6082 |
T8971 |
T8967 |
compound |
Purification,kit |
R6083 |
T8972 |
T8973 |
punct |
(,Pierce |
R6084 |
T8973 |
T8967 |
parataxis |
Pierce,kit |
R6085 |
T8974 |
T8973 |
punct |
", ",Pierce |
R6086 |
T8975 |
T8973 |
npadvmod |
http://www.piercenet.com,Pierce |
R6087 |
T8976 |
T8973 |
punct |
),Pierce |
R6088 |
T8977 |
T8961 |
punct |
.,isolated |
R6089 |
T8979 |
T8980 |
compound |
IgY,antibodies |
R6090 |
T8980 |
T8981 |
nsubjpass |
antibodies,purified |
R6091 |
T8982 |
T8981 |
auxpass |
were,purified |
R6092 |
T8983 |
T8981 |
advmod |
then,purified |
R6093 |
T8984 |
T8981 |
dep |
affinity,purified |
R6094 |
T8985 |
T8981 |
advcl |
using,purified |
R6095 |
T8986 |
T8987 |
det |
the,peptide |
R6096 |
T8987 |
T8985 |
dobj |
peptide,using |
R6097 |
T8988 |
T8987 |
amod |
immunizing,peptide |
R6098 |
T8989 |
T8987 |
amod |
synthetic,peptide |
R6099 |
T8990 |
T8981 |
punct |
.,purified |