PMC:1941754 / 39200-46401 JSON TXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T8150	23-26	NN	denotes	PCR
T8151	27-35	NN	denotes	analysis
T8152	36-38	IN	denotes	of
T8153	39-45	NN	denotes	Trip13
T8154	46-50	NN	denotes	cDNA
T8155	50-51	.	denotes	.
T8156	51-269	sentence	denotes	Trip13 was amplified from samples of Clontech's Mouse Multiple Tissue cDNA Panel I (http://www.clontech.com), using the following primers: 5′-GCACCATTGCACTTCACATC-3′ (TRP3-6F) and 5′-TGACCATCAGACTGTCGAGC-3′ (TRP3-6R).
T8157	52-58	NN	denotes	Trip13
T8159	59-62	VBD	denotes	was
T8158	63-72	VBN	denotes	amplified
T8160	73-77	IN	denotes	from
T8161	78-85	NNS	denotes	samples
T8162	86-88	IN	denotes	of
T8163	89-97	NNP	denotes	Clontech
T8165	97-99	POS	denotes	's
T8166	100-105	NN	denotes	Mouse
T8167	106-114	JJ	denotes	Multiple
T8168	115-121	NN	denotes	Tissue
T8169	122-126	NN	denotes	cDNA
T8164	127-132	NN	denotes	Panel
T8170	133-134	CD	denotes	I
T8171	135-136	-LRB-	denotes	(
T8172	136-159	NN	denotes	http://www.clontech.com
T8173	159-160	-RRB-	denotes	)
T8174	160-162	,	denotes	,
T8175	162-167	VBG	denotes	using
T8176	168-171	DT	denotes	the
T8178	172-181	VBG	denotes	following
T8177	182-189	NNS	denotes	primers
T8179	189-191	:	denotes	:
T8180	191-192	CD	denotes	5
T8182	192-193	SYM	denotes	′
T8183	193-194	HYPH	denotes	-
T8181	194-214	NN	denotes	GCACCATTGCACTTCACATC
T8184	214-215	HYPH	denotes	-
T8185	215-216	CD	denotes	3
T8186	216-217	SYM	denotes	′
T8187	218-219	-LRB-	denotes	(
T8188	219-223	NN	denotes	TRP3
T8190	223-224	HYPH	denotes	-
T8189	224-226	NN	denotes	6F
T8191	226-227	-RRB-	denotes	)
T8192	228-231	CC	denotes	and
T8193	232-233	CD	denotes	5
T8195	233-234	SYM	denotes	′
T8196	234-235	HYPH	denotes	-
T8194	235-255	NN	denotes	TGACCATCAGACTGTCGAGC
T8197	255-256	HYPH	denotes	-
T8198	256-257	CD	denotes	3
T8199	257-258	SYM	denotes	′
T8200	259-260	-LRB-	denotes	(
T8201	260-264	NN	denotes	TRP3
T8203	264-265	HYPH	denotes	-
T8202	265-267	NN	denotes	6R
T8204	267-268	-RRB-	denotes	)
T8205	268-269	.	denotes	.
T8206	269-361	sentence	denotes	These primers correspond to exons 3 and 6, respectively, and amplify a 330-bp cDNA product.
T8207	270-275	DT	denotes	These
T8208	276-283	NNS	denotes	primers
T8209	284-294	VBP	denotes	correspond
T8210	295-297	IN	denotes	to
T8211	298-303	NNS	denotes	exons
T8212	304-305	CD	denotes	3
T8213	306-309	CC	denotes	and
T8214	310-311	CD	denotes	6
T8215	311-313	,	denotes	,
T8216	313-325	RB	denotes	respectively
T8217	325-327	,	denotes	,
T8218	327-330	CC	denotes	and
T8219	331-338	VB	denotes	amplify
T8220	339-340	DT	denotes	a
T8222	341-344	CD	denotes	330
T8224	344-345	HYPH	denotes	-
T8223	345-347	NN	denotes	bp
T8225	348-352	NN	denotes	cDNA
T8221	353-360	NN	denotes	product
T8226	360-361	.	denotes	.
T8227	361-441	sentence	denotes	The cDNAs in this panel are equalized to allow quantitative analysis by RT-PCR.
T8228	362-365	DT	denotes	The
T8229	366-371	NNS	denotes	cDNAs
T8231	372-374	IN	denotes	in
T8232	375-379	DT	denotes	this
T8233	380-385	NN	denotes	panel
T8234	386-389	VBP	denotes	are
T8230	390-399	VBN	denotes	equalized
T8235	400-402	TO	denotes	to
T8236	403-408	VB	denotes	allow
T8237	409-421	JJ	denotes	quantitative
T8238	422-430	NN	denotes	analysis
T8239	431-433	IN	denotes	by
T8240	434-436	NN	denotes	RT
T8242	436-437	HYPH	denotes	-
T8241	437-440	NN	denotes	PCR
T8243	440-441	.	denotes	.
T8308	443-453	NN	denotes	Generation
T8309	454-456	IN	denotes	of
T8310	457-463	NN	denotes	Trip13
T8312	463-464	HYPH	denotes	-
T8311	464-473	JJ	denotes	deficient
T8313	474-478	NNS	denotes	mice
T8314	478-479	.	denotes	.
T8315	479-647	sentence	denotes	The mouse embryonic stem cell line RRB047 (strain 129/Ola) containing a gene trap insertion in Trip13 was obtained from BayGenomics (http://www.baygenomics.ucsf.edu/).
T8316	480-483	DT	denotes	The
T8318	484-489	NN	denotes	mouse
T8319	490-499	JJ	denotes	embryonic
T8320	500-504	NN	denotes	stem
T8321	505-509	NN	denotes	cell
T8322	510-514	NN	denotes	line
T8317	515-521	NN	denotes	RRB047
T8324	522-523	-LRB-	denotes	(
T8325	523-529	NN	denotes	strain
T8327	530-533	CD	denotes	129
T8328	533-534	HYPH	denotes	/
T8326	534-537	NN	denotes	Ola
T8329	537-538	-RRB-	denotes	)
T8330	539-549	VBG	denotes	containing
T8331	550-551	DT	denotes	a
T8333	552-556	NN	denotes	gene
T8334	557-561	NN	denotes	trap
T8332	562-571	NN	denotes	insertion
T8335	572-574	IN	denotes	in
T8336	575-581	NN	denotes	Trip13
T8337	582-585	VBD	denotes	was
T8323	586-594	VBN	denotes	obtained
T8338	595-599	IN	denotes	from
T8339	600-611	NNP	denotes	BayGenomics
T8340	612-613	-LRB-	denotes	(
T8341	613-645	NN	denotes	http://www.baygenomics.ucsf.edu/
T8342	645-646	-RRB-	denotes	)
T8343	646-647	.	denotes	.
T8344	647-877	sentence	denotes	The gene-trapping vector used to create this line, pGT1lxf, was designed to create an in-frame fusion between the 5′ exons of the trapped gene and a reporter, βgeo (a fusion of β-galactosidase and neomycin phosphotransferase II).
T8345	648-651	DT	denotes	The
T8347	652-656	NN	denotes	gene
T8348	656-657	HYPH	denotes	-
T8349	657-665	VBG	denotes	trapping
T8346	666-672	NN	denotes	vector
T8351	673-677	VBN	denotes	used
T8352	678-680	TO	denotes	to
T8353	681-687	VB	denotes	create
T8354	688-692	DT	denotes	this
T8355	693-697	NN	denotes	line
T8356	697-699	,	denotes	,
T8357	699-706	NN	denotes	pGT1lxf
T8358	706-708	,	denotes	,
T8359	708-711	VBD	denotes	was
T8350	712-720	VBN	denotes	designed
T8360	721-723	TO	denotes	to
T8361	724-730	VB	denotes	create
T8362	731-733	DT	denotes	an
T8364	734-736	IN	denotes	in
T8365	736-737	HYPH	denotes	-
T8366	737-742	NN	denotes	frame
T8363	743-749	NN	denotes	fusion
T8367	750-757	IN	denotes	between
T8368	758-761	DT	denotes	the
T8370	762-763	CD	denotes	5
T8371	763-764	SYM	denotes	′
T8369	765-770	NNS	denotes	exons
T8372	771-773	IN	denotes	of
T8373	774-777	DT	denotes	the
T8375	778-785	VBN	denotes	trapped
T8374	786-790	NN	denotes	gene
T8376	791-794	CC	denotes	and
T8377	795-796	DT	denotes	a
T8378	797-805	NN	denotes	reporter
T8379	805-807	,	denotes	,
T8380	807-811	NN	denotes	βgeo
T8381	812-813	-LRB-	denotes	(
T8382	813-814	DT	denotes	a
T8383	815-821	NN	denotes	fusion
T8384	822-824	IN	denotes	of
T8385	825-826	NN	denotes	β
T8387	826-827	HYPH	denotes	-
T8386	827-840	NN	denotes	galactosidase
T8388	841-844	CC	denotes	and
T8389	845-853	NN	denotes	neomycin
T8390	854-872	NN	denotes	phosphotransferase
T8391	873-875	CD	denotes	II
T8392	875-876	-RRB-	denotes	)
T8393	876-877	.	denotes	.
T8394	877-969	sentence	denotes	The gene-trapped locus creates a fusion transcript containing exons 1–3 of Trip13 and βgeo.
T8395	878-881	DT	denotes	The
T8397	882-886	NN	denotes	gene
T8399	886-887	HYPH	denotes	-
T8398	887-894	VBN	denotes	trapped
T8396	895-900	NN	denotes	locus
T8400	901-908	VBZ	denotes	creates
T8401	909-910	DT	denotes	a
T8403	911-917	NN	denotes	fusion
T8402	918-928	NN	denotes	transcript
T8404	929-939	VBG	denotes	containing
T8405	940-945	NNS	denotes	exons
T8407	946-947	CD	denotes	1
T8408	947-948	SYM	denotes	–
T8406	948-949	CD	denotes	3
T8409	950-952	IN	denotes	of
T8410	953-959	NN	denotes	Trip13
T8411	960-963	CC	denotes	and
T8412	964-968	NN	denotes	βgeo
T8413	968-969	.	denotes	.
T8414	969-1182	sentence	denotes	To identify the exact insertion site within intron 3, PCR was performed using one primer within the gene trap vector, and the other primer at various positions in intron 3 pointing towards the 3′ end of the gene.
T8415	970-972	TO	denotes	To
T8416	973-981	VB	denotes	identify
T8418	982-985	DT	denotes	the
T8420	986-991	JJ	denotes	exact
T8421	992-1001	NN	denotes	insertion
T8419	1002-1006	NN	denotes	site
T8422	1007-1013	IN	denotes	within
T8423	1014-1020	NN	denotes	intron
T8424	1021-1022	CD	denotes	3
T8425	1022-1024	,	denotes	,
T8426	1024-1027	NN	denotes	PCR
T8427	1028-1031	VBD	denotes	was
T8417	1032-1041	VBN	denotes	performed
T8428	1042-1047	VBG	denotes	using
T8429	1048-1051	CD	denotes	one
T8430	1052-1058	NN	denotes	primer
T8431	1059-1065	IN	denotes	within
T8432	1066-1069	DT	denotes	the
T8434	1070-1074	NN	denotes	gene
T8435	1075-1079	NN	denotes	trap
T8433	1080-1086	NN	denotes	vector
T8436	1086-1088	,	denotes	,
T8437	1088-1091	CC	denotes	and
T8438	1092-1095	DT	denotes	the
T8440	1096-1101	JJ	denotes	other
T8439	1102-1108	NN	denotes	primer
T8441	1109-1111	IN	denotes	at
T8442	1112-1119	JJ	denotes	various
T8443	1120-1129	NNS	denotes	positions
T8444	1130-1132	IN	denotes	in
T8445	1133-1139	NN	denotes	intron
T8446	1140-1141	CD	denotes	3
T8447	1142-1150	VBG	denotes	pointing
T8448	1151-1158	IN	denotes	towards
T8449	1159-1162	DT	denotes	the
T8451	1163-1164	CD	denotes	3
T8452	1164-1165	SYM	denotes	′
T8450	1166-1169	NN	denotes	end
T8453	1170-1172	IN	denotes	of
T8454	1173-1176	DT	denotes	the
T8455	1177-1181	NN	denotes	gene
T8456	1181-1182	.	denotes	.
T8457	1182-1292	sentence	denotes	Product from a productive reaction was sequenced, revealing that the insertion site was 445 bp into intron 3.
T8458	1183-1190	NN	denotes	Product
T8460	1191-1195	IN	denotes	from
T8461	1196-1197	DT	denotes	a
T8463	1198-1208	JJ	denotes	productive
T8462	1209-1217	NN	denotes	reaction
T8464	1218-1221	VBD	denotes	was
T8459	1222-1231	VBN	denotes	sequenced
T8465	1231-1233	,	denotes	,
T8466	1233-1242	VBG	denotes	revealing
T8467	1243-1247	IN	denotes	that
T8469	1248-1251	DT	denotes	the
T8471	1252-1261	NN	denotes	insertion
T8470	1262-1266	NN	denotes	site
T8468	1267-1270	VBD	denotes	was
T8472	1271-1274	CD	denotes	445
T8473	1275-1277	NN	denotes	bp
T8474	1278-1282	IN	denotes	into
T8475	1283-1289	NN	denotes	intron
T8476	1290-1291	CD	denotes	3
T8477	1291-1292	.	denotes	.
T8521	1294-1304	NN	denotes	Genotyping
T8522	1305-1307	IN	denotes	of
T8523	1308-1312	NNS	denotes	mice
T8524	1312-1313	.	denotes	.
T8525	1313-1527	sentence	denotes	Three primers were used to distinguish wild-type and mutant alleles of Trip13: primer 1, 5′-CGTCGCTCCATTGCTTTGTGC-3′; primer 2, 5′-AGTAGTGGTACACTGTATTTTTGCTTTCATTGA-3′; and primer 3, 5′-GTAGATCCCGGCGCTCTTACCAA-3′.
T8526	1314-1319	CD	denotes	Three
T8527	1320-1327	NNS	denotes	primers
T8529	1328-1332	VBD	denotes	were
T8528	1333-1337	VBN	denotes	used
T8530	1338-1340	TO	denotes	to
T8531	1341-1352	VB	denotes	distinguish
T8532	1353-1357	JJ	denotes	wild
T8534	1357-1358	HYPH	denotes	-
T8533	1358-1362	NN	denotes	type
T8536	1363-1366	CC	denotes	and
T8537	1367-1373	NN	denotes	mutant
T8535	1374-1381	NNS	denotes	alleles
T8538	1382-1384	IN	denotes	of
T8539	1385-1391	NN	denotes	Trip13
T8540	1391-1393	:	denotes	:
T8541	1393-1399	NN	denotes	primer
T8542	1400-1401	CD	denotes	1
T8543	1401-1403	,	denotes	,
T8544	1403-1404	CD	denotes	5
T8546	1404-1405	SYM	denotes	′
T8547	1405-1406	HYPH	denotes	-
T8545	1406-1427	NN	denotes	CGTCGCTCCATTGCTTTGTGC
T8548	1427-1428	HYPH	denotes	-
T8549	1428-1429	CD	denotes	3
T8550	1429-1430	SYM	denotes	′
T8551	1430-1431	:	denotes	;
T8552	1432-1438	NN	denotes	primer
T8553	1439-1440	CD	denotes	2
T8554	1440-1442	,	denotes	,
T8555	1442-1443	CD	denotes	5
T8557	1443-1444	SYM	denotes	′
T8558	1444-1445	HYPH	denotes	-
T8556	1445-1478	NN	denotes	AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
T8559	1478-1479	HYPH	denotes	-
T8560	1479-1480	CD	denotes	3
T8561	1480-1481	SYM	denotes	′
T8562	1481-1482	:	denotes	;
T8563	1483-1486	CC	denotes	and
T8564	1487-1493	NN	denotes	primer
T8565	1494-1495	CD	denotes	3
T8566	1495-1497	,	denotes	,
T8567	1497-1498	CD	denotes	5
T8569	1498-1499	SYM	denotes	′
T8570	1499-1500	HYPH	denotes	-
T8568	1500-1523	NN	denotes	GTAGATCCCGGCGCTCTTACCAA
T8571	1523-1524	HYPH	denotes	-
T8572	1524-1525	CD	denotes	3
T8573	1525-1526	SYM	denotes	′
T8574	1526-1527	.	denotes	.
T8575	1527-1642	sentence	denotes	Primers 1 and 2 are located upstream and downstream, respectively, of the gene trap insertion within the intron 3.
T8576	1528-1535	NNS	denotes	Primers
T8577	1536-1537	CD	denotes	1
T8579	1538-1541	CC	denotes	and
T8580	1542-1543	CD	denotes	2
T8581	1544-1547	VBP	denotes	are
T8578	1548-1555	VBN	denotes	located
T8582	1556-1564	RB	denotes	upstream
T8583	1565-1568	CC	denotes	and
T8584	1569-1579	RB	denotes	downstream
T8585	1579-1581	,	denotes	,
T8586	1581-1593	RB	denotes	respectively
T8587	1593-1595	,	denotes	,
T8588	1595-1597	IN	denotes	of
T8589	1598-1601	DT	denotes	the
T8591	1602-1606	NN	denotes	gene
T8592	1607-1611	NN	denotes	trap
T8590	1612-1621	NN	denotes	insertion
T8593	1622-1628	IN	denotes	within
T8594	1629-1632	DT	denotes	the
T8595	1633-1639	NN	denotes	intron
T8596	1640-1641	CD	denotes	3
T8597	1641-1642	.	denotes	.
T8598	1642-1683	sentence	denotes	Primer 3 corresponds to pGTlxf sequence.
T8599	1643-1649	NN	denotes	Primer
T8601	1650-1651	CD	denotes	3
T8600	1652-1663	VBZ	denotes	corresponds
T8602	1664-1666	IN	denotes	to
T8603	1667-1673	NN	denotes	pGTlxf
T8604	1674-1682	NN	denotes	sequence
T8605	1682-1683	.	denotes	.
T8606	1683-1810	sentence	denotes	Primers 1 and 2 amplify a 700-bp band from a wild-type allele; primers 1 and 3 amplify a 540-bp fragment from a mutant allele.
T8607	1684-1691	NNS	denotes	Primers
T8608	1692-1693	CD	denotes	1
T8610	1694-1697	CC	denotes	and
T8611	1698-1699	CD	denotes	2
T8609	1700-1707	VBP	denotes	amplify
T8613	1708-1709	DT	denotes	a
T8615	1710-1713	CD	denotes	700
T8617	1713-1714	HYPH	denotes	-
T8616	1714-1716	NN	denotes	bp
T8614	1717-1721	NN	denotes	band
T8618	1722-1726	IN	denotes	from
T8619	1727-1728	DT	denotes	a
T8621	1729-1733	JJ	denotes	wild
T8623	1733-1734	HYPH	denotes	-
T8622	1734-1738	NN	denotes	type
T8620	1739-1745	NN	denotes	allele
T8624	1745-1746	:	denotes	;
T8625	1747-1754	NNS	denotes	primers
T8626	1755-1756	CD	denotes	1
T8627	1757-1760	CC	denotes	and
T8628	1761-1762	CD	denotes	3
T8612	1763-1770	VBP	denotes	amplify
T8629	1771-1772	DT	denotes	a
T8631	1773-1776	CD	denotes	540
T8633	1776-1777	HYPH	denotes	-
T8632	1777-1779	NN	denotes	bp
T8630	1780-1788	NN	denotes	fragment
T8634	1789-1793	IN	denotes	from
T8635	1794-1795	DT	denotes	a
T8637	1796-1802	NN	denotes	mutant
T8636	1803-1809	NN	denotes	allele
T8638	1809-1810	.	denotes	.
T8639	1810-1908	sentence	denotes	Separate reactions were used to assay the presence or absence of each amplicon from a DNA sample.
T8640	1811-1819	JJ	denotes	Separate
T8641	1820-1829	NNS	denotes	reactions
T8643	1830-1834	VBD	denotes	were
T8642	1835-1839	VBN	denotes	used
T8644	1840-1842	TO	denotes	to
T8645	1843-1848	VB	denotes	assay
T8646	1849-1852	DT	denotes	the
T8647	1853-1861	NN	denotes	presence
T8648	1862-1864	CC	denotes	or
T8649	1865-1872	NN	denotes	absence
T8650	1873-1875	IN	denotes	of
T8651	1876-1880	DT	denotes	each
T8652	1881-1889	NN	denotes	amplicon
T8653	1890-1894	IN	denotes	from
T8654	1895-1896	DT	denotes	a
T8656	1897-1900	NN	denotes	DNA
T8655	1901-1907	NN	denotes	sample
T8657	1907-1908	.	denotes	.
T8658	1908-2019	sentence	denotes	The cycling conditions were: 94 °C 2 min; 35 cycles of 94 °C 30 s, 57 °C 45 s, and 72°C 50 s; and 72 °C 2 min.
T8659	1909-1912	DT	denotes	The
T8661	1913-1920	NN	denotes	cycling
T8660	1921-1931	NNS	denotes	conditions
T8662	1932-1936	VBD	denotes	were
T8663	1936-1938	:	denotes	:
T8664	1938-1940	CD	denotes	94
T8665	1941-1943	NN	denotes	°C
T8666	1944-1945	CD	denotes	2
T8667	1946-1949	NN	denotes	min
T8668	1949-1950	:	denotes	;
T8669	1951-1953	CD	denotes	35
T8670	1954-1960	NNS	denotes	cycles
T8671	1961-1963	IN	denotes	of
T8672	1964-1966	CD	denotes	94
T8673	1967-1969	NN	denotes	°C
T8674	1970-1972	CD	denotes	30
T8675	1973-1974	NN	denotes	s
T8676	1974-1976	,	denotes	,
T8677	1976-1978	CD	denotes	57
T8678	1979-1981	NN	denotes	°C
T8679	1982-1984	CD	denotes	45
T8680	1985-1986	NN	denotes	s
T8681	1986-1988	,	denotes	,
T8682	1988-1991	CC	denotes	and
T8683	1992-1994	CD	denotes	72
T8684	1994-1996	NN	denotes	°C
T8685	1997-1999	CD	denotes	50
T8686	2000-2001	NN	denotes	s
T8687	2001-2002	:	denotes	;
T8688	2003-2006	CC	denotes	and
T8689	2007-2009	CD	denotes	72
T8690	2010-2012	NN	denotes	°C
T8691	2013-2014	CD	denotes	2
T8692	2015-2018	NN	denotes	min
T8693	2018-2019	.	denotes	.
T8717	2021-2023	NN	denotes	RT
T8719	2023-2024	HYPH	denotes	-
T8718	2024-2027	NN	denotes	PCR
T8720	2027-2028	.	denotes	.
T8721	2028-2243	sentence	denotes	Total RNA was isolated from adult testes with the RNeasy Mini Kit (Qiagen, http://www.qiagen.com), and 4.0 μg was oligo dT–primed and reverse-transcribed with Superscript II (Stratagene, http://www.stratagene.com).
T8722	2029-2034	JJ	denotes	Total
T8723	2035-2038	NN	denotes	RNA
T8725	2039-2042	VBD	denotes	was
T8724	2043-2051	VBN	denotes	isolated
T8726	2052-2056	IN	denotes	from
T8727	2057-2062	JJ	denotes	adult
T8728	2063-2069	NNS	denotes	testes
T8729	2070-2074	IN	denotes	with
T8730	2075-2078	DT	denotes	the
T8732	2079-2085	NNP	denotes	RNeasy
T8733	2086-2090	NNP	denotes	Mini
T8731	2091-2094	NNP	denotes	Kit
T8734	2095-2096	-LRB-	denotes	(
T8735	2096-2102	NNP	denotes	Qiagen
T8736	2102-2104	,	denotes	,
T8737	2104-2125	NN	denotes	http://www.qiagen.com
T8738	2125-2126	-RRB-	denotes	)
T8739	2126-2128	,	denotes	,
T8740	2128-2131	CC	denotes	and
T8741	2132-2135	CD	denotes	4.0
T8742	2136-2138	NN	denotes	μg
T8744	2139-2142	VBD	denotes	was
T8745	2143-2148	NN	denotes	oligo
T8746	2149-2151	NN	denotes	dT
T8747	2151-2152	HYPH	denotes	–
T8743	2152-2158	VBN	denotes	primed
T8748	2159-2162	CC	denotes	and
T8749	2163-2170	RB	denotes	reverse
T8751	2170-2171	HYPH	denotes	-
T8750	2171-2182	VBN	denotes	transcribed
T8752	2183-2187	IN	denotes	with
T8753	2188-2199	NNP	denotes	Superscript
T8754	2200-2202	CD	denotes	II
T8755	2203-2204	-LRB-	denotes	(
T8756	2204-2214	NNP	denotes	Stratagene
T8757	2214-2216	,	denotes	,
T8758	2216-2241	NN	denotes	http://www.stratagene.com
T8759	2241-2242	-RRB-	denotes	)
T8760	2242-2243	.	denotes	.
T8761	2243-2366	sentence	denotes	The entire Trip13 protein-coding sequence was amplified with primers 5′-ATGGACGAGGCGGTG-3′ and 5′-TCAAACATAAGCTGAAAGTT-3′.
T8762	2244-2247	DT	denotes	The
T8764	2248-2254	JJ	denotes	entire
T8765	2255-2261	NN	denotes	Trip13
T8766	2262-2269	NN	denotes	protein
T8768	2269-2270	HYPH	denotes	-
T8767	2270-2276	VBG	denotes	coding
T8763	2277-2285	NN	denotes	sequence
T8770	2286-2289	VBD	denotes	was
T8769	2290-2299	VBN	denotes	amplified
T8771	2300-2304	IN	denotes	with
T8772	2305-2312	NNS	denotes	primers
T8774	2313-2314	CD	denotes	5
T8775	2314-2315	SYM	denotes	′
T8776	2315-2316	HYPH	denotes	-
T8773	2316-2331	NN	denotes	ATGGACGAGGCGGTG
T8777	2331-2332	HYPH	denotes	-
T8778	2332-2333	CD	denotes	3
T8779	2333-2334	SYM	denotes	′
T8780	2335-2338	CC	denotes	and
T8781	2339-2340	CD	denotes	5
T8783	2340-2341	SYM	denotes	′
T8784	2341-2342	HYPH	denotes	-
T8782	2342-2362	NN	denotes	TCAAACATAAGCTGAAAGTT
T8785	2362-2363	HYPH	denotes	-
T8786	2363-2364	CD	denotes	3
T8787	2364-2365	SYM	denotes	′
T8788	2365-2366	.	denotes	.
T8789	2366-2478	sentence	denotes	The cycling conditions were: 94 °C 2 min; 94 °C 30s, 55 °C 45 s, and 72 °C 80 s for 35 cycles; and 72 °C 2 min.
T8790	2367-2370	DT	denotes	The
T8792	2371-2378	NN	denotes	cycling
T8791	2379-2389	NNS	denotes	conditions
T8793	2390-2394	VBD	denotes	were
T8794	2394-2396	:	denotes	:
T8795	2396-2398	CD	denotes	94
T8796	2399-2401	NN	denotes	°C
T8797	2402-2403	CD	denotes	2
T8798	2404-2407	NN	denotes	min
T8799	2407-2408	:	denotes	;
T8800	2409-2411	CD	denotes	94
T8801	2412-2414	NN	denotes	°C
T8802	2415-2417	CD	denotes	30
T8803	2417-2418	NN	denotes	s
T8804	2418-2420	,	denotes	,
T8805	2420-2422	CD	denotes	55
T8806	2423-2425	NN	denotes	°C
T8807	2426-2428	CD	denotes	45
T8808	2429-2430	NN	denotes	s
T8809	2430-2432	,	denotes	,
T8810	2432-2435	CC	denotes	and
T8811	2436-2438	CD	denotes	72
T8812	2439-2441	NN	denotes	°C
T8813	2442-2444	CD	denotes	80
T8814	2445-2446	NN	denotes	s
T8815	2447-2450	IN	denotes	for
T8816	2451-2453	CD	denotes	35
T8817	2454-2460	NNS	denotes	cycles
T8818	2460-2461	:	denotes	;
T8819	2462-2465	CC	denotes	and
T8820	2466-2468	CD	denotes	72
T8821	2469-2471	NN	denotes	°C
T8822	2472-2473	CD	denotes	2
T8823	2474-2477	NN	denotes	min
T8824	2477-2478	.	denotes	.
T8825	2478-2624	sentence	denotes	The primers for amplifying the Med31 coding sequence as control were : 5′-ATGGCCGCGGCCGTCGCTATGG-3′ and 5′-TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC-3′.
T8826	2479-2482	DT	denotes	The
T8827	2483-2490	NNS	denotes	primers
T8829	2491-2494	IN	denotes	for
T8830	2495-2505	VBG	denotes	amplifying
T8831	2506-2509	DT	denotes	the
T8833	2510-2515	NN	denotes	Med31
T8834	2516-2522	NN	denotes	coding
T8832	2523-2531	NN	denotes	sequence
T8835	2532-2534	IN	denotes	as
T8836	2535-2542	NN	denotes	control
T8828	2543-2547	VBD	denotes	were
T8837	2548-2550	:	denotes	:
T8838	2550-2551	CD	denotes	5
T8840	2551-2552	SYM	denotes	′
T8841	2552-2553	HYPH	denotes	-
T8839	2553-2575	NN	denotes	ATGGCCGCGGCCGTCGCTATGG
T8842	2575-2576	HYPH	denotes	-
T8843	2576-2577	CD	denotes	3
T8844	2577-2578	SYM	denotes	′
T8845	2579-2582	CC	denotes	and
T8846	2583-2584	CD	denotes	5
T8848	2584-2585	SYM	denotes	′
T8849	2585-2586	HYPH	denotes	-
T8847	2586-2620	NN	denotes	TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC
T8850	2620-2621	HYPH	denotes	-
T8851	2621-2622	CD	denotes	3
T8852	2622-2623	SYM	denotes	′
T8853	2623-2624	.	denotes	.
T8854	2624-2737	sentence	denotes	The cycling conditions were: 94 °C 2 min; 94 °C 30 s, 55 °C 30 s, and 72 °C 35 s for 35 cycles; and 72 °C 2 min.
T8855	2625-2628	DT	denotes	The
T8857	2629-2636	NN	denotes	cycling
T8856	2637-2647	NNS	denotes	conditions
T8858	2648-2652	VBD	denotes	were
T8859	2652-2654	:	denotes	:
T8860	2654-2656	CD	denotes	94
T8861	2657-2659	NN	denotes	°C
T8862	2660-2661	CD	denotes	2
T8863	2662-2665	NN	denotes	min
T8864	2665-2666	:	denotes	;
T8865	2667-2669	CD	denotes	94
T8866	2670-2672	NN	denotes	°C
T8867	2673-2675	CD	denotes	30
T8868	2676-2677	NN	denotes	s
T8869	2677-2679	,	denotes	,
T8870	2679-2681	CD	denotes	55
T8871	2682-2684	NN	denotes	°C
T8872	2685-2687	CD	denotes	30
T8873	2688-2689	NN	denotes	s
T8874	2689-2691	,	denotes	,
T8875	2691-2694	CC	denotes	and
T8876	2695-2697	CD	denotes	72
T8877	2698-2700	NN	denotes	°C
T8878	2701-2703	CD	denotes	35
T8879	2704-2705	NN	denotes	s
T8880	2706-2709	IN	denotes	for
T8881	2710-2712	CD	denotes	35
T8882	2713-2719	NNS	denotes	cycles
T8883	2719-2720	:	denotes	;
T8884	2721-2724	CC	denotes	and
T8885	2725-2727	CD	denotes	72
T8886	2728-2730	NN	denotes	°C
T8887	2731-2732	CD	denotes	2
T8888	2733-2736	NN	denotes	min
T8889	2736-2737	.	denotes	.
T8918	2739-2750	NN	denotes	Development
T8919	2751-2754	CC	denotes	and
T8920	2755-2767	NN	denotes	purification
T8921	2768-2770	IN	denotes	of
T8922	2771-2778	NN	denotes	chicken
T8923	2779-2789	NNS	denotes	antibodies
T8924	2789-2790	.	denotes	.
T8925	2790-2969	sentence	denotes	A peptide corresponding to amino acids 25–40 of TRIP13, VLQRSGSTAKKEDIK, was conjugated to KLH and used to immunize chickens (done by Sigma Genosys, http://www.sigmaaldrich.com).
T8926	2791-2792	DT	denotes	A
T8927	2793-2800	NN	denotes	peptide
T8929	2801-2814	VBG	denotes	corresponding
T8930	2815-2817	IN	denotes	to
T8931	2818-2823	NN	denotes	amino
T8933	2824-2829	NNS	denotes	acids
T8932	2830-2832	CD	denotes	25
T8934	2832-2833	SYM	denotes	–
T8935	2833-2835	CD	denotes	40
T8936	2836-2838	IN	denotes	of
T8937	2839-2845	NN	denotes	TRIP13
T8938	2845-2847	,	denotes	,
T8939	2847-2862	NN	denotes	VLQRSGSTAKKEDIK
T8940	2862-2864	,	denotes	,
T8941	2864-2867	VBD	denotes	was
T8928	2868-2878	VBN	denotes	conjugated
T8942	2879-2881	IN	denotes	to
T8943	2882-2885	NN	denotes	KLH
T8944	2886-2889	CC	denotes	and
T8945	2890-2894	VBN	denotes	used
T8946	2895-2897	TO	denotes	to
T8947	2898-2906	VB	denotes	immunize
T8948	2907-2915	NNS	denotes	chickens
T8949	2916-2917	-LRB-	denotes	(
T8950	2917-2921	VBN	denotes	done
T8951	2922-2924	IN	denotes	by
T8952	2925-2930	NNP	denotes	Sigma
T8953	2931-2938	NNP	denotes	Genosys
T8954	2938-2940	,	denotes	,
T8955	2940-2967	NN	denotes	http://www.sigmaaldrich.com
T8956	2967-2968	-RRB-	denotes	)
T8957	2968-2969	.	denotes	.
T8958	2969-3092	sentence	denotes	Polyclonal IgY was isolated from eggs with the Eggcellent Chicken IgY Purification kit (Pierce, http://www.piercenet.com).
T8959	2970-2980	JJ	denotes	Polyclonal
T8960	2981-2984	NN	denotes	IgY
T8962	2985-2988	VBD	denotes	was
T8961	2989-2997	VBN	denotes	isolated
T8963	2998-3002	IN	denotes	from
T8964	3003-3007	NNS	denotes	eggs
T8965	3008-3012	IN	denotes	with
T8966	3013-3016	DT	denotes	the
T8968	3017-3027	NNP	denotes	Eggcellent
T8969	3028-3035	NNP	denotes	Chicken
T8970	3036-3039	NNP	denotes	IgY
T8971	3040-3052	NNP	denotes	Purification
T8967	3053-3056	NN	denotes	kit
T8972	3057-3058	-LRB-	denotes	(
T8973	3058-3064	NNP	denotes	Pierce
T8974	3064-3066	,	denotes	,
T8975	3066-3090	NN	denotes	http://www.piercenet.com
T8976	3090-3091	-RRB-	denotes	)
T8977	3091-3092	.	denotes	.
T8978	3092-3175	sentence	denotes	IgY antibodies were then affinity purified using the immunizing synthetic peptide.
T8979	3093-3096	NN	denotes	IgY
T8980	3097-3107	NNS	denotes	antibodies
T8982	3108-3112	VBD	denotes	were
T8983	3113-3117	RB	denotes	then
T8984	3118-3126	NN	denotes	affinity
T8981	3127-3135	VBN	denotes	purified
T8985	3136-3141	VBG	denotes	using
T8986	3142-3145	DT	denotes	the
T8988	3146-3156	VBG	denotes	immunizing
T8989	3157-3166	JJ	denotes	synthetic
T8987	3167-3174	NN	denotes	peptide
T8990	3174-3175	.	denotes	.
T9024	3177-3184	NNP	denotes	Western
T9025	3185-3193	NN	denotes	blotting
T9026	3193-3194	.	denotes	.
T9027	3194-3355	sentence	denotes	50 μg of testis extract in RIPA buffer was separated by 8% SDS-PAGE and electrotransferred onto a Pure Nitrocellulose membrane (Bio-Rad, http://www.biorad.com).
T9028	3195-3197	CD	denotes	50
T9029	3198-3200	NN	denotes	μg
T9031	3201-3203	IN	denotes	of
T9032	3204-3210	NN	denotes	testis
T9033	3211-3218	NN	denotes	extract
T9034	3219-3221	IN	denotes	in
T9035	3222-3226	NN	denotes	RIPA
T9036	3227-3233	NN	denotes	buffer
T9037	3234-3237	VBD	denotes	was
T9030	3238-3247	VBN	denotes	separated
T9038	3248-3250	IN	denotes	by
T9039	3251-3252	CD	denotes	8
T9040	3252-3253	NN	denotes	%
T9042	3254-3257	NN	denotes	SDS
T9043	3257-3258	HYPH	denotes	-
T9041	3258-3262	NN	denotes	PAGE
T9044	3263-3266	CC	denotes	and
T9045	3267-3285	VBD	denotes	electrotransferred
T9046	3286-3290	IN	denotes	onto
T9047	3291-3292	DT	denotes	a
T9049	3293-3297	NNP	denotes	Pure
T9050	3298-3312	NNP	denotes	Nitrocellulose
T9048	3313-3321	NN	denotes	membrane
T9051	3322-3323	-LRB-	denotes	(
T9053	3323-3326	NNP	denotes	Bio
T9054	3326-3327	HYPH	denotes	-
T9052	3327-3330	NNP	denotes	Rad
T9055	3330-3332	,	denotes	,
T9056	3332-3353	NN	denotes	http://www.biorad.com
T9057	3353-3354	-RRB-	denotes	)
T9058	3354-3355	.	denotes	.
T9059	3355-3484	sentence	denotes	The membrane was incubated with a polyclonal rabbit anti-human TRIP13 antibody (18-003-42687; Genway, http://www.genwaybio.com).
T9060	3356-3359	DT	denotes	The
T9061	3360-3368	NN	denotes	membrane
T9063	3369-3372	VBD	denotes	was
T9062	3373-3382	VBN	denotes	incubated
T9064	3383-3387	IN	denotes	with
T9065	3388-3389	DT	denotes	a
T9067	3390-3400	JJ	denotes	polyclonal
T9068	3401-3407	NN	denotes	rabbit
T9069	3408-3418	JJ	denotes	anti-human
T9070	3419-3425	NN	denotes	TRIP13
T9066	3426-3434	NN	denotes	antibody
T9071	3435-3436	-LRB-	denotes	(
T9073	3436-3438	CD	denotes	18
T9075	3438-3439	HYPH	denotes	-
T9076	3439-3442	CD	denotes	003
T9077	3442-3443	HYPH	denotes	-
T9074	3443-3448	CD	denotes	42687
T9078	3448-3449	:	denotes	;
T9072	3450-3456	NNP	denotes	Genway
T9079	3456-3458	,	denotes	,
T9080	3458-3482	NN	denotes	http://www.genwaybio.com
T9081	3482-3483	-RRB-	denotes	)
T9082	3483-3484	.	denotes	.
T9083	3484-3610	sentence	denotes	According to the manufacturer, the immunogen was a synthetic peptide embedded in sequence we deduced to correspond to exon 3.
T9084	3485-3494	VBG	denotes	According
T9086	3495-3497	IN	denotes	to
T9087	3498-3501	DT	denotes	the
T9088	3502-3514	NN	denotes	manufacturer
T9089	3514-3516	,	denotes	,
T9090	3516-3519	DT	denotes	the
T9091	3520-3529	NN	denotes	immunogen
T9085	3530-3533	VBD	denotes	was
T9092	3534-3535	DT	denotes	a
T9094	3536-3545	JJ	denotes	synthetic
T9093	3546-3553	NN	denotes	peptide
T9095	3554-3562	VBN	denotes	embedded
T9096	3563-3565	IN	denotes	in
T9097	3566-3574	NN	denotes	sequence
T9098	3575-3577	PRP	denotes	we
T9099	3578-3585	VBD	denotes	deduced
T9100	3586-3588	TO	denotes	to
T9101	3589-3599	VB	denotes	correspond
T9102	3600-3602	IN	denotes	to
T9103	3603-3607	NN	denotes	exon
T9104	3608-3609	CD	denotes	3
T9105	3609-3610	.	denotes	.
T9106	3610-3744	sentence	denotes	Binding was detected by chemiluminescence ECL kit (Pierce) using a rabbit anti-chicken IgG horseradish peroxidase conjugate (Pierce).
T9107	3611-3618	NN	denotes	Binding
T9109	3619-3622	VBD	denotes	was
T9108	3623-3631	VBN	denotes	detected
T9110	3632-3634	IN	denotes	by
T9111	3635-3652	NN	denotes	chemiluminescence
T9113	3653-3656	NN	denotes	ECL
T9112	3657-3660	NN	denotes	kit
T9114	3661-3662	-LRB-	denotes	(
T9115	3662-3668	NNP	denotes	Pierce
T9116	3668-3669	-RRB-	denotes	)
T9117	3670-3675	VBG	denotes	using
T9118	3676-3677	DT	denotes	a
T9120	3678-3684	NN	denotes	rabbit
T9121	3685-3697	JJ	denotes	anti-chicken
T9122	3698-3701	NN	denotes	IgG
T9123	3702-3713	NN	denotes	horseradish
T9124	3714-3724	NN	denotes	peroxidase
T9119	3725-3734	NN	denotes	conjugate
T9125	3735-3736	-LRB-	denotes	(
T9126	3736-3742	NNP	denotes	Pierce
T9127	3742-3743	-RRB-	denotes	)
T9128	3743-3744	.	denotes	.
T9148	3746-3758	JJ	denotes	Histological
T9149	3759-3767	NNS	denotes	analyses
T9150	3767-3768	.	denotes	.
T9151	3768-3888	sentence	denotes	Testes or ovaries were fixed in Bouin's, embedded in paraffin, sectioned at 6 μm, and stained by hematoxylin and eosin.
T9152	3769-3775	NNS	denotes	Testes
T9154	3776-3778	CC	denotes	or
T9155	3779-3786	NNS	denotes	ovaries
T9156	3787-3791	VBD	denotes	were
T9153	3792-3797	VBN	denotes	fixed
T9157	3798-3800	IN	denotes	in
T9158	3801-3806	NNP	denotes	Bouin
T9159	3806-3808	POS	denotes	's
T9160	3808-3810	,	denotes	,
T9161	3810-3818	VBN	denotes	embedded
T9162	3819-3821	IN	denotes	in
T9163	3822-3830	NN	denotes	paraffin
T9164	3830-3832	,	denotes	,
T9165	3832-3841	VBN	denotes	sectioned
T9166	3842-3844	IN	denotes	at
T9167	3845-3846	CD	denotes	6
T9168	3847-3849	NN	denotes	μm
T9169	3849-3851	,	denotes	,
T9170	3851-3854	CC	denotes	and
T9171	3855-3862	VBN	denotes	stained
T9172	3863-3865	IN	denotes	by
T9173	3866-3877	NN	denotes	hematoxylin
T9174	3878-3881	CC	denotes	and
T9175	3882-3887	NN	denotes	eosin
T9176	3887-3888	.	denotes	.
T9177	3888-3973	sentence	denotes	Antigen retrieval for immunohistochemistry of testis sections was as described [60].
T9178	3889-3896	NN	denotes	Antigen
T9179	3897-3906	NN	denotes	retrieval
T9181	3907-3910	IN	denotes	for
T9182	3911-3931	NN	denotes	immunohistochemistry
T9183	3932-3934	IN	denotes	of
T9184	3935-3941	NN	denotes	testis
T9185	3942-3950	NNS	denotes	sections
T9180	3951-3954	VBD	denotes	was
T9186	3955-3957	IN	denotes	as
T9187	3958-3967	VBN	denotes	described
T9188	3968-3969	-LRB-	denotes	[
T9189	3969-3971	CD	denotes	60
T9190	3971-3972	-RRB-	denotes	]
T9191	3972-3973	.	denotes	.
T9192	3973-4033	sentence	denotes	Oocyte and follicle numbers were counted as described [61].
T9193	3974-3980	NN	denotes	Oocyte
T9195	3981-3984	CC	denotes	and
T9196	3985-3993	NN	denotes	follicle
T9194	3994-4001	NNS	denotes	numbers
T9198	4002-4006	VBD	denotes	were
T9197	4007-4014	VBN	denotes	counted
T9199	4015-4017	IN	denotes	as
T9200	4018-4027	VBN	denotes	described
T9201	4028-4029	-LRB-	denotes	[
T9202	4029-4031	CD	denotes	61
T9203	4031-4032	-RRB-	denotes	]
T9204	4032-4033	.	denotes	.
T9205	4033-4113	sentence	denotes	Only follicles containing an oocyte with a clearly visible nucleus were scored.
T9206	4034-4038	RB	denotes	Only
T9207	4039-4048	NNS	denotes	follicles
T9209	4049-4059	VBG	denotes	containing
T9210	4060-4062	DT	denotes	an
T9211	4063-4069	NN	denotes	oocyte
T9212	4070-4074	IN	denotes	with
T9213	4075-4076	DT	denotes	a
T9215	4077-4084	RB	denotes	clearly
T9216	4085-4092	JJ	denotes	visible
T9214	4093-4100	NN	denotes	nucleus
T9217	4101-4105	VBD	denotes	were
T9208	4106-4112	VBN	denotes	scored
T9218	4112-4113	.	denotes	.
T9322	4115-4134	NN	denotes	Immunocytochemistry
T9323	4134-4135	.	denotes	.
T9324	4135-4230	sentence	denotes	Immunolabeling of surface-spread spermatocytes and oocytes was performed as described [39,62].
T9325	4136-4150	NN	denotes	Immunolabeling
T9327	4151-4153	IN	denotes	of
T9328	4154-4161	NN	denotes	surface
T9330	4161-4162	HYPH	denotes	-
T9331	4162-4168	NN	denotes	spread
T9329	4169-4182	NNS	denotes	spermatocytes
T9332	4183-4186	CC	denotes	and
T9333	4187-4194	NNS	denotes	oocytes
T9334	4195-4198	VBD	denotes	was
T9326	4199-4208	VBN	denotes	performed
T9335	4209-4211	IN	denotes	as
T9336	4212-4221	VBN	denotes	described
T9337	4222-4223	-LRB-	denotes	[
T9339	4223-4225	CD	denotes	39
T9340	4225-4226	,	denotes	,
T9338	4226-4228	CD	denotes	62
T9341	4228-4229	-RRB-	denotes	]
T9342	4229-4230	.	denotes	.
T9343	4230-4606	sentence	denotes	To reach conclusions on the pattern of staining for various proteins, 30 (unless otherwise indicated) well-spread nuclei of particular meiotic stages were first identified under the fluorescent microscope on the basis of SYCP3 or STAG3 staining, then imaged at both appropriate wavelengths to determine the pattern of second proteins with focal patterns such as RAD51 or RPA.
T9344	4231-4233	TO	denotes	To
T9345	4234-4239	VB	denotes	reach
T9347	4240-4251	NNS	denotes	conclusions
T9348	4252-4254	IN	denotes	on
T9349	4255-4258	DT	denotes	the
T9350	4259-4266	NN	denotes	pattern
T9351	4267-4269	IN	denotes	of
T9352	4270-4278	NN	denotes	staining
T9353	4279-4282	IN	denotes	for
T9354	4283-4290	JJ	denotes	various
T9355	4291-4299	NN	denotes	proteins
T9356	4299-4301	,	denotes	,
T9357	4301-4303	CD	denotes	30
T9359	4304-4305	-LRB-	denotes	(
T9361	4305-4311	IN	denotes	unless
T9362	4312-4321	RB	denotes	otherwise
T9360	4322-4331	VBN	denotes	indicated
T9363	4331-4332	-RRB-	denotes	)
T9364	4333-4337	RB	denotes	well
T9366	4337-4338	HYPH	denotes	-
T9365	4338-4344	VBN	denotes	spread
T9358	4345-4351	NNS	denotes	nuclei
T9367	4352-4354	IN	denotes	of
T9368	4355-4365	JJ	denotes	particular
T9370	4366-4373	JJ	denotes	meiotic
T9369	4374-4380	NNS	denotes	stages
T9371	4381-4385	VBD	denotes	were
T9372	4386-4391	RB	denotes	first
T9346	4392-4402	VBN	denotes	identified
T9373	4403-4408	IN	denotes	under
T9374	4409-4412	DT	denotes	the
T9376	4413-4424	JJ	denotes	fluorescent
T9375	4425-4435	NN	denotes	microscope
T9377	4436-4438	IN	denotes	on
T9378	4439-4442	DT	denotes	the
T9379	4443-4448	NN	denotes	basis
T9380	4449-4451	IN	denotes	of
T9381	4452-4457	NN	denotes	SYCP3
T9383	4458-4460	CC	denotes	or
T9384	4461-4466	NN	denotes	STAG3
T9382	4467-4475	NN	denotes	staining
T9385	4475-4477	,	denotes	,
T9386	4477-4481	RB	denotes	then
T9387	4482-4488	VBN	denotes	imaged
T9388	4489-4491	IN	denotes	at
T9389	4492-4496	DT	denotes	both
T9391	4497-4508	JJ	denotes	appropriate
T9390	4509-4520	NNS	denotes	wavelengths
T9392	4521-4523	TO	denotes	to
T9393	4524-4533	VB	denotes	determine
T9394	4534-4537	DT	denotes	the
T9395	4538-4545	NN	denotes	pattern
T9396	4546-4548	IN	denotes	of
T9397	4549-4555	JJ	denotes	second
T9398	4556-4564	NN	denotes	proteins
T9399	4565-4569	IN	denotes	with
T9400	4570-4575	JJ	denotes	focal
T9401	4576-4584	NNS	denotes	patterns
T9402	4585-4589	JJ	denotes	such
T9403	4590-4592	IN	denotes	as
T9404	4593-4598	NN	denotes	RAD51
T9405	4599-4601	CC	denotes	or
T9406	4602-4605	NN	denotes	RPA
T9407	4605-4606	.	denotes	.
T9408	4606-4715	sentence	denotes	Unless otherwise indicated, the panels shown in the figures were the exclusive or predominant patterns seen.
T9409	4607-4613	IN	denotes	Unless
T9411	4614-4623	RB	denotes	otherwise
T9410	4624-4633	VBN	denotes	indicated
T9413	4633-4635	,	denotes	,
T9414	4635-4638	DT	denotes	the
T9415	4639-4645	NNS	denotes	panels
T9416	4646-4651	VBN	denotes	shown
T9417	4652-4654	IN	denotes	in
T9418	4655-4658	DT	denotes	the
T9419	4659-4666	NNS	denotes	figures
T9412	4667-4671	VBD	denotes	were
T9420	4672-4675	DT	denotes	the
T9422	4676-4685	JJ	denotes	exclusive
T9423	4686-4688	CC	denotes	or
T9424	4689-4700	JJ	denotes	predominant
T9421	4701-4709	NNS	denotes	patterns
T9425	4710-4714	VBN	denotes	seen
T9426	4714-4715	.	denotes	.
T9427	4715-4878	sentence	denotes	The exception for this approach was in the case of staining for MLH1 or MLH3 plus RAD51 (in which case SYCP3 or STAG3 was not available to find chromosome cores).
T9428	4716-4719	DT	denotes	The
T9429	4720-4729	NN	denotes	exception
T9431	4730-4733	IN	denotes	for
T9432	4734-4738	DT	denotes	this
T9433	4739-4747	NN	denotes	approach
T9430	4748-4751	VBD	denotes	was
T9434	4752-4754	IN	denotes	in
T9435	4755-4758	DT	denotes	the
T9436	4759-4763	NN	denotes	case
T9437	4764-4766	IN	denotes	of
T9438	4767-4775	VBG	denotes	staining
T9439	4776-4779	IN	denotes	for
T9440	4780-4784	NN	denotes	MLH1
T9441	4785-4787	CC	denotes	or
T9442	4788-4792	NN	denotes	MLH3
T9443	4793-4797	CC	denotes	plus
T9444	4798-4803	NN	denotes	RAD51
T9445	4804-4805	-LRB-	denotes	(
T9446	4805-4807	IN	denotes	in
T9448	4808-4813	WDT	denotes	which
T9449	4814-4818	NN	denotes	case
T9450	4819-4824	NN	denotes	SYCP3
T9451	4825-4827	CC	denotes	or
T9452	4828-4833	NN	denotes	STAG3
T9447	4834-4837	VBD	denotes	was
T9453	4838-4841	RB	denotes	not
T9454	4842-4851	JJ	denotes	available
T9455	4852-4854	TO	denotes	to
T9456	4855-4859	VB	denotes	find
T9457	4860-4870	NN	denotes	chromosome
T9458	4871-4876	NNS	denotes	cores
T9459	4876-4877	-RRB-	denotes	)
T9460	4877-4878	.	denotes	.
T9461	4878-4998	sentence	denotes	Nuclei in this situation were identified first by MLH1/3 foci clustering, then imaged for both fluorescent wavelengths.
T9462	4879-4885	NNS	denotes	Nuclei
T9464	4886-4888	IN	denotes	in
T9465	4889-4893	DT	denotes	this
T9466	4894-4903	NN	denotes	situation
T9467	4904-4908	VBD	denotes	were
T9463	4909-4919	VBN	denotes	identified
T9468	4920-4925	RB	denotes	first
T9469	4926-4928	IN	denotes	by
T9470	4929-4933	NN	denotes	MLH1
T9472	4933-4934	HYPH	denotes	/
T9473	4934-4935	CD	denotes	3
T9471	4936-4940	NNS	denotes	foci
T9474	4941-4951	VBG	denotes	clustering
T9475	4951-4953	,	denotes	,
T9476	4953-4957	RB	denotes	then
T9477	4958-4964	VBN	denotes	imaged
T9478	4965-4968	IN	denotes	for
T9479	4969-4973	DT	denotes	both
T9481	4974-4985	JJ	denotes	fluorescent
T9480	4986-4997	NNS	denotes	wavelengths
T9482	4997-4998	.	denotes	.
T9483	4998-5838	sentence	denotes	Primary antibodies used in this study were as follows: mouse anti-SCP3 (1:500; Abcam, http://www.abcam.com); rabbit anti-SYCP1 (1:1,000; a gift from C. Heyting) [63]; rabbit anti-REC8 (1:100; a gift from C. Heyting); rabbit anti-RAD51 (1:250, this polyclonal antibody recognizes both RAD51 and DMC1; Oncogene Research Products, http://www.merckbiosciences.co.uk); rabbit anti-γH2AX (1:500; Upstate Biotechnology, http://www.upstate.com/); rabbit anti-STAG3 (1:1,000; a gift from R. Jessberger); rabbit anti-MLH3 (1:400; a gift from P. Cohen); mouse-anti-human MLH1 (1:50; BD Biosciences, http://www.bdbiosciences.com); rabbit-anti-TopBP1 (1:100; a gift from J. Chen) [22]; mouse-anti-ubiquityl-histone H2A (1:200; Upstate Biotechnology); rabbit-anti-TRF2 (1:500; a gift from T. de Lange); and rabbit-anti-BLM (1:50; a gift from R. Freire).
T9484	4999-5006	JJ	denotes	Primary
T9485	5007-5017	NNS	denotes	antibodies
T9487	5018-5022	VBN	denotes	used
T9488	5023-5025	IN	denotes	in
T9489	5026-5030	DT	denotes	this
T9490	5031-5036	NN	denotes	study
T9486	5037-5041	VBD	denotes	were
T9491	5042-5044	IN	denotes	as
T9492	5045-5052	VBZ	denotes	follows
T9493	5052-5054	:	denotes	:
T9494	5054-5059	NN	denotes	mouse
T9495	5060-5069	JJ	denotes	anti-SCP3
T9496	5070-5071	-LRB-	denotes	(
T9498	5071-5072	CD	denotes	1
T9499	5072-5073	SYM	denotes	:
T9500	5073-5076	CD	denotes	500
T9501	5076-5077	:	denotes	;
T9497	5078-5083	NNP	denotes	Abcam
T9502	5083-5085	,	denotes	,
T9503	5085-5105	NN	denotes	http://www.abcam.com
T9504	5105-5106	-RRB-	denotes	)
T9505	5106-5107	:	denotes	;
T9506	5108-5114	NN	denotes	rabbit
T9507	5115-5125	NN	denotes	anti-SYCP1
T9508	5126-5127	-LRB-	denotes	(
T9510	5127-5128	CD	denotes	1
T9511	5128-5129	SYM	denotes	:
T9512	5129-5134	CD	denotes	1,000
T9513	5134-5135	:	denotes	;
T9514	5136-5137	DT	denotes	a
T9509	5138-5142	NN	denotes	gift
T9515	5143-5147	IN	denotes	from
T9516	5148-5150	NNP	denotes	C.
T9517	5151-5158	NNP	denotes	Heyting
T9518	5158-5159	-RRB-	denotes	)
T9519	5160-5161	-LRB-	denotes	[
T9520	5161-5163	CD	denotes	63
T9521	5163-5164	-RRB-	denotes	]
T9522	5164-5165	:	denotes	;
T9523	5166-5172	NN	denotes	rabbit
T9524	5173-5182	NN	denotes	anti-REC8
T9525	5183-5184	-LRB-	denotes	(
T9527	5184-5185	CD	denotes	1
T9528	5185-5186	SYM	denotes	:
T9529	5186-5189	CD	denotes	100
T9530	5189-5190	:	denotes	;
T9531	5191-5192	DT	denotes	a
T9526	5193-5197	NN	denotes	gift
T9532	5198-5202	IN	denotes	from
T9533	5203-5205	NNP	denotes	C.
T9534	5206-5213	NNP	denotes	Heyting
T9535	5213-5214	-RRB-	denotes	)
T9536	5214-5215	:	denotes	;
T9537	5216-5222	NN	denotes	rabbit
T9538	5223-5233	NN	denotes	anti-RAD51
T9539	5234-5235	-LRB-	denotes	(
T9541	5235-5236	CD	denotes	1
T9542	5236-5237	SYM	denotes	:
T9543	5237-5240	CD	denotes	250
T9544	5240-5242	,	denotes	,
T9545	5242-5246	DT	denotes	this
T9547	5247-5257	JJ	denotes	polyclonal
T9546	5258-5266	NN	denotes	antibody
T9548	5267-5277	VBZ	denotes	recognizes
T9549	5278-5282	CC	denotes	both
T9550	5283-5288	NN	denotes	RAD51
T9551	5289-5292	CC	denotes	and
T9552	5293-5297	NN	denotes	DMC1
T9553	5297-5298	:	denotes	;
T9554	5299-5307	NNP	denotes	Oncogene
T9555	5308-5316	NNP	denotes	Research
T9540	5317-5325	NNP	denotes	Products
T9556	5325-5327	,	denotes	,
T9557	5327-5360	NN	denotes	http://www.merckbiosciences.co.uk
T9558	5360-5361	-RRB-	denotes	)
T9559	5361-5362	:	denotes	;
T9560	5363-5369	NN	denotes	rabbit
T9561	5370-5380	NN	denotes	anti-γH2AX
T9562	5381-5382	-LRB-	denotes	(
T9564	5382-5383	CD	denotes	1
T9565	5383-5384	SYM	denotes	:
T9566	5384-5387	CD	denotes	500
T9567	5387-5388	:	denotes	;
T9568	5389-5396	NNP	denotes	Upstate
T9563	5397-5410	NNP	denotes	Biotechnology
T9569	5410-5412	,	denotes	,
T9570	5412-5435	NN	denotes	http://www.upstate.com/
T9571	5435-5436	-RRB-	denotes	)
T9572	5436-5437	:	denotes	;
T9573	5438-5444	NN	denotes	rabbit
T9574	5445-5455	NN	denotes	anti-STAG3
T9575	5456-5457	-LRB-	denotes	(
T9577	5457-5458	CD	denotes	1
T9578	5458-5459	SYM	denotes	:
T9579	5459-5464	CD	denotes	1,000
T9580	5464-5465	:	denotes	;
T9581	5466-5467	DT	denotes	a
T9576	5468-5472	NN	denotes	gift
T9582	5473-5477	IN	denotes	from
T9583	5478-5480	NNP	denotes	R.
T9584	5481-5491	NNP	denotes	Jessberger
T9585	5491-5492	-RRB-	denotes	)
T9586	5492-5493	:	denotes	;
T9587	5494-5500	NN	denotes	rabbit
T9588	5501-5510	NN	denotes	anti-MLH3
T9589	5511-5512	-LRB-	denotes	(
T9591	5512-5513	CD	denotes	1
T9592	5513-5514	SYM	denotes	:
T9593	5514-5517	CD	denotes	400
T9594	5517-5518	:	denotes	;
T9595	5519-5520	DT	denotes	a
T9590	5521-5525	NN	denotes	gift
T9596	5526-5530	IN	denotes	from
T9597	5531-5533	NNP	denotes	P.
T9598	5534-5539	NNP	denotes	Cohen
T9599	5539-5540	-RRB-	denotes	)
T9600	5540-5541	:	denotes	;
T9601	5542-5547	JJ	denotes	mouse
T9603	5547-5548	HYPH	denotes	-
T9604	5548-5558	JJ	denotes	anti-human
T9602	5559-5563	NN	denotes	MLH1
T9605	5564-5565	-LRB-	denotes	(
T9607	5565-5566	CD	denotes	1
T9608	5566-5567	SYM	denotes	:
T9609	5567-5569	CD	denotes	50
T9610	5569-5570	:	denotes	;
T9611	5571-5573	NNP	denotes	BD
T9606	5574-5585	NNP	denotes	Biosciences
T9612	5585-5587	,	denotes	,
T9613	5587-5615	NN	denotes	http://www.bdbiosciences.com
T9614	5615-5616	-RRB-	denotes	)
T9615	5616-5617	:	denotes	;
T9616	5618-5624	NN	denotes	rabbit
T9618	5624-5625	HYPH	denotes	-
T9617	5625-5636	NN	denotes	anti-TopBP1
T9619	5637-5638	-LRB-	denotes	(
T9621	5638-5639	CD	denotes	1
T9622	5639-5640	SYM	denotes	:
T9623	5640-5643	CD	denotes	100
T9624	5643-5644	:	denotes	;
T9625	5645-5646	DT	denotes	a
T9620	5647-5651	NN	denotes	gift
T9626	5652-5656	IN	denotes	from
T9627	5657-5659	NNP	denotes	J.
T9628	5660-5664	NNP	denotes	Chen
T9629	5664-5665	-RRB-	denotes	)
T9630	5666-5667	-LRB-	denotes	[
T9631	5667-5669	CD	denotes	22
T9632	5669-5670	-RRB-	denotes	]
T9633	5670-5671	:	denotes	;
T9634	5672-5677	NN	denotes	mouse
T9636	5677-5678	HYPH	denotes	-
T9637	5678-5692	JJ	denotes	anti-ubiquityl
T9638	5692-5693	HYPH	denotes	-
T9639	5693-5700	NN	denotes	histone
T9635	5701-5704	NN	denotes	H2A
T9640	5705-5706	-LRB-	denotes	(
T9642	5706-5707	CD	denotes	1
T9643	5707-5708	SYM	denotes	:
T9644	5708-5711	CD	denotes	200
T9645	5711-5712	:	denotes	;
T9646	5713-5720	NNP	denotes	Upstate
T9641	5721-5734	NNP	denotes	Biotechnology
T9647	5734-5735	-RRB-	denotes	)
T9648	5735-5736	:	denotes	;
T9649	5737-5743	NN	denotes	rabbit
T9651	5743-5744	HYPH	denotes	-
T9650	5744-5753	NN	denotes	anti-TRF2
T9652	5754-5755	-LRB-	denotes	(
T9654	5755-5756	CD	denotes	1
T9655	5756-5757	SYM	denotes	:
T9656	5757-5760	CD	denotes	500
T9657	5760-5761	:	denotes	;
T9658	5762-5763	DT	denotes	a
T9653	5764-5768	NN	denotes	gift
T9659	5769-5773	IN	denotes	from
T9660	5774-5776	NNP	denotes	T.
T9662	5777-5779	NNP	denotes	de
T9661	5780-5785	NNP	denotes	Lange
T9663	5785-5786	-RRB-	denotes	)
T9664	5786-5787	:	denotes	;
T9665	5788-5791	CC	denotes	and
T9666	5792-5798	NN	denotes	rabbit
T9668	5798-5799	HYPH	denotes	-
T9667	5799-5807	NN	denotes	anti-BLM
T9669	5808-5809	-LRB-	denotes	(
T9671	5809-5810	CD	denotes	1
T9672	5810-5811	SYM	denotes	:
T9673	5811-5813	CD	denotes	50
T9674	5813-5814	:	denotes	;
T9675	5815-5816	DT	denotes	a
T9670	5817-5821	NN	denotes	gift
T9676	5822-5826	IN	denotes	from
T9677	5827-5829	NNP	denotes	R.
T9678	5830-5836	NNP	denotes	Freire
T9679	5836-5837	-RRB-	denotes	)
T9680	5837-5838	.	denotes	.
T9681	5838-5995	sentence	denotes	All secondary antibodies conjugated with either Alexa Fluor 488 or 594 (Molecular Probes, http://probes.invitrogen.com/) were used at a dilution of 1:1,000.
T9682	5839-5842	DT	denotes	All
T9684	5843-5852	JJ	denotes	secondary
T9683	5853-5863	NNS	denotes	antibodies
T9686	5864-5874	VBN	denotes	conjugated
T9687	5875-5879	IN	denotes	with
T9688	5880-5886	CC	denotes	either
T9690	5887-5892	NNP	denotes	Alexa
T9691	5893-5898	NNP	denotes	Fluor
T9689	5899-5902	CD	denotes	488
T9692	5903-5905	CC	denotes	or
T9693	5906-5909	CD	denotes	594
T9694	5910-5911	-LRB-	denotes	(
T9696	5911-5920	NNP	denotes	Molecular
T9695	5921-5927	NNP	denotes	Probes
T9697	5927-5929	,	denotes	,
T9698	5929-5958	NN	denotes	http://probes.invitrogen.com/
T9699	5958-5959	-RRB-	denotes	)
T9700	5960-5964	VBD	denotes	were
T9685	5965-5969	VBN	denotes	used
T9701	5970-5972	IN	denotes	at
T9702	5973-5974	DT	denotes	a
T9703	5975-5983	NN	denotes	dilution
T9704	5984-5986	IN	denotes	of
T9705	5987-5988	CD	denotes	1
T9706	5988-5989	SYM	denotes	:
T9707	5989-5994	CD	denotes	1,000
T9708	5994-5995	.	denotes	.
T9709	5995-6065	sentence	denotes	All images were taken with a 100× objective lens under immersion oil.
T9710	5996-5999	DT	denotes	All
T9711	6000-6006	NNS	denotes	images
T9713	6007-6011	VBD	denotes	were
T9712	6012-6017	VBN	denotes	taken
T9714	6018-6022	IN	denotes	with
T9715	6023-6024	DT	denotes	a
T9717	6025-6028	CD	denotes	100
T9718	6028-6029	SYM	denotes	×
T9719	6030-6039	NN	denotes	objective
T9716	6040-6044	NN	denotes	lens
T9720	6045-6050	IN	denotes	under
T9721	6051-6060	NN	denotes	immersion
T9722	6061-6064	NN	denotes	oil
T9723	6064-6065	.	denotes	.
T9753	6067-6076	NN	denotes	Metaphase
T9755	6077-6078	CD	denotes	I
T9754	6079-6091	NN	denotes	spermatocyte
T9756	6092-6099	NNS	denotes	spreads
T9757	6100-6103	CC	denotes	and
T9758	6104-6106	NN	denotes	OA
T9759	6107-6116	NN	denotes	treatment
T9760	6116-6117	.	denotes	.
T9761	6117-6286	sentence	denotes	Metaphase fixed spermatocytes from 8-mo-old Trip13RRB047 homozygotes, using 23-d-old wild-type mice as control, were prepared and stained with Giemsa as described [64].
T9762	6118-6127	NN	denotes	Metaphase
T9763	6128-6133	VBN	denotes	fixed
T9764	6134-6147	NNS	denotes	spermatocytes
T9766	6148-6152	IN	denotes	from
T9767	6153-6154	CD	denotes	8
T9769	6154-6155	HYPH	denotes	-
T9768	6155-6157	NN	denotes	mo
T9771	6157-6158	HYPH	denotes	-
T9770	6158-6161	JJ	denotes	old
T9773	6162-6174	NN	denotes	Trip13RRB047
T9772	6175-6186	NNS	denotes	homozygotes
T9774	6186-6188	,	denotes	,
T9775	6188-6193	VBG	denotes	using
T9776	6194-6196	CD	denotes	23
T9778	6196-6197	HYPH	denotes	-
T9777	6197-6198	NN	denotes	d
T9780	6198-6199	HYPH	denotes	-
T9779	6199-6202	JJ	denotes	old
T9782	6203-6207	JJ	denotes	wild
T9784	6207-6208	HYPH	denotes	-
T9783	6208-6212	NN	denotes	type
T9781	6213-6217	NNS	denotes	mice
T9785	6218-6220	IN	denotes	as
T9786	6221-6228	NN	denotes	control
T9787	6228-6230	,	denotes	,
T9788	6230-6234	VBD	denotes	were
T9765	6235-6243	VBN	denotes	prepared
T9789	6244-6247	CC	denotes	and
T9790	6248-6255	VBN	denotes	stained
T9791	6256-6260	IN	denotes	with
T9792	6261-6267	NNP	denotes	Giemsa
T9793	6268-6270	IN	denotes	as
T9794	6271-6280	VBN	denotes	described
T9795	6281-6282	-LRB-	denotes	[
T9796	6282-6284	CD	denotes	64
T9797	6284-6285	-RRB-	denotes	]
T9798	6285-6286	.	denotes	.
T9799	6286-6456	sentence	denotes	For OA treatment, cells were exposed to 5 μM OA (Calbiochem, http://www.emdbiosciences.com) for 6 h at 32 °C in a humidified environment of 5% CO2 before spreading [65].
T9800	6287-6290	IN	denotes	For
T9802	6291-6293	NN	denotes	OA
T9803	6294-6303	NN	denotes	treatment
T9804	6303-6305	,	denotes	,
T9805	6305-6310	NNS	denotes	cells
T9806	6311-6315	VBD	denotes	were
T9801	6316-6323	VBN	denotes	exposed
T9807	6324-6326	IN	denotes	to
T9808	6327-6328	CD	denotes	5
T9809	6329-6331	NN	denotes	μM
T9810	6332-6334	NN	denotes	OA
T9811	6335-6336	-LRB-	denotes	(
T9812	6336-6346	NNP	denotes	Calbiochem
T9813	6346-6348	,	denotes	,
T9814	6348-6377	NN	denotes	http://www.emdbiosciences.com
T9815	6377-6378	-RRB-	denotes	)
T9816	6379-6382	IN	denotes	for
T9817	6383-6384	CD	denotes	6
T9818	6385-6386	NN	denotes	h
T9819	6387-6389	IN	denotes	at
T9820	6390-6392	CD	denotes	32
T9821	6393-6395	NN	denotes	°C
T9822	6396-6398	IN	denotes	in
T9823	6399-6400	DT	denotes	a
T9825	6401-6411	JJ	denotes	humidified
T9824	6412-6423	NN	denotes	environment
T9826	6424-6426	IN	denotes	of
T9827	6427-6428	CD	denotes	5
T9828	6428-6429	NN	denotes	%
T9829	6430-6433	NN	denotes	CO2
T9830	6434-6440	IN	denotes	before
T9831	6441-6450	VBG	denotes	spreading
T9832	6451-6452	-LRB-	denotes	[
T9833	6452-6454	CD	denotes	65
T9834	6454-6455	-RRB-	denotes	]
T9835	6455-6456	.	denotes	.
T9836	6456-6545	sentence	denotes	These preparations were stained with DAPI to visualize metaphase nuclei and chromosomes.
T9837	6457-6462	DT	denotes	These
T9838	6463-6475	NNS	denotes	preparations
T9840	6476-6480	VBD	denotes	were
T9839	6481-6488	VBN	denotes	stained
T9841	6489-6493	IN	denotes	with
T9842	6494-6498	NN	denotes	DAPI
T9843	6499-6501	TO	denotes	to
T9844	6502-6511	VB	denotes	visualize
T9845	6512-6521	NN	denotes	metaphase
T9846	6522-6528	NNS	denotes	nuclei
T9847	6529-6532	CC	denotes	and
T9848	6533-6544	NNS	denotes	chromosomes
T9849	6544-6545	.	denotes	.
T9864	6547-6559	JJ	denotes	Phylogenetic
T9865	6560-6568	NNS	denotes	analyses
T9866	6568-6569	.	denotes	.
T9867	6569-6689	sentence	denotes	TRIP13 orthologs were identified by BLASTP searches of Genbank and other sources providing gene models such as Ensembl.
T9868	6570-6576	NN	denotes	TRIP13
T9869	6577-6586	NNS	denotes	orthologs
T9871	6587-6591	VBD	denotes	were
T9870	6592-6602	VBN	denotes	identified
T9872	6603-6605	IN	denotes	by
T9873	6606-6612	NNP	denotes	BLASTP
T9874	6613-6621	NNS	denotes	searches
T9875	6622-6624	IN	denotes	of
T9876	6625-6632	NNP	denotes	Genbank
T9877	6633-6636	CC	denotes	and
T9878	6637-6642	JJ	denotes	other
T9879	6643-6650	NNS	denotes	sources
T9880	6651-6660	VBG	denotes	providing
T9881	6661-6665	NN	denotes	gene
T9882	6666-6672	NNS	denotes	models
T9883	6673-6677	JJ	denotes	such
T9884	6678-6680	IN	denotes	as
T9885	6681-6688	NNP	denotes	Ensembl
T9886	6688-6689	.	denotes	.
T9887	6689-6738	sentence	denotes	The selected orthologs can be found in Table S1.
T9888	6690-6693	DT	denotes	The
T9890	6694-6702	VBN	denotes	selected
T9889	6703-6712	NNS	denotes	orthologs
T9892	6713-6716	MD	denotes	can
T9893	6717-6719	VB	denotes	be
T9891	6720-6725	VBN	denotes	found
T9894	6726-6728	IN	denotes	in
T9895	6729-6734	NN	denotes	Table
T9896	6735-6737	NN	denotes	S1
T9897	6737-6738	.	denotes	.
T9898	6738-6893	sentence	denotes	Amino acid alignments were done with Clustal W, using the default settings with and without removing the regions outside of the AAA-ATPase central domain.
T9899	6739-6744	NN	denotes	Amino
T9900	6745-6749	NN	denotes	acid
T9901	6750-6760	NNS	denotes	alignments
T9903	6761-6765	VBD	denotes	were
T9902	6766-6770	VBN	denotes	done
T9904	6771-6775	IN	denotes	with
T9905	6776-6783	NNP	denotes	Clustal
T9906	6784-6785	NNP	denotes	W
T9907	6785-6787	,	denotes	,
T9908	6787-6792	VBG	denotes	using
T9909	6793-6796	DT	denotes	the
T9911	6797-6804	NN	denotes	default
T9910	6805-6813	NNS	denotes	settings
T9912	6814-6818	IN	denotes	with
T9913	6819-6822	CC	denotes	and
T9914	6823-6830	IN	denotes	without
T9915	6831-6839	VBG	denotes	removing
T9916	6840-6843	DT	denotes	the
T9917	6844-6851	NNS	denotes	regions
T9918	6852-6859	IN	denotes	outside
T9919	6860-6862	IN	denotes	of
T9920	6863-6866	DT	denotes	the
T9922	6867-6870	NN	denotes	AAA
T9924	6870-6871	HYPH	denotes	-
T9923	6871-6877	NN	denotes	ATPase
T9925	6878-6885	JJ	denotes	central
T9921	6886-6892	NN	denotes	domain
T9926	6892-6893	.	denotes	.
T9927	6893-6982	sentence	denotes	The trees were constructed by using the neighbor-joining method with Poisson correction.
T9928	6894-6897	DT	denotes	The
T9929	6898-6903	NNS	denotes	trees
T9931	6904-6908	VBD	denotes	were
T9930	6909-6920	VBN	denotes	constructed
T9932	6921-6923	IN	denotes	by
T9933	6924-6929	VBG	denotes	using
T9934	6930-6933	DT	denotes	the
T9936	6934-6942	NN	denotes	neighbor
T9938	6942-6943	HYPH	denotes	-
T9937	6943-6950	VBG	denotes	joining
T9935	6951-6957	NN	denotes	method
T9939	6958-6962	IN	denotes	with
T9940	6963-6970	NNP	denotes	Poisson
T9941	6971-6981	NN	denotes	correction
T9942	6981-6982	.	denotes	.
T9943	6982-7118	sentence	denotes	The reliability of internal branches was assessed by using 500 bootstrap replicates, and sites with gaps were ignored in this analysis.
T9944	6983-6986	DT	denotes	The
T9945	6987-6998	NN	denotes	reliability
T9947	6999-7001	IN	denotes	of
T9948	7002-7010	JJ	denotes	internal
T9949	7011-7019	NNS	denotes	branches
T9950	7020-7023	VBD	denotes	was
T9946	7024-7032	VBN	denotes	assessed
T9951	7033-7035	IN	denotes	by
T9952	7036-7041	VBG	denotes	using
T9953	7042-7045	CD	denotes	500
T9955	7046-7055	NN	denotes	bootstrap
T9954	7056-7066	NNS	denotes	replicates
T9956	7066-7068	,	denotes	,
T9957	7068-7071	CC	denotes	and
T9958	7072-7077	NNS	denotes	sites
T9960	7078-7082	IN	denotes	with
T9961	7083-7087	NNS	denotes	gaps
T9962	7088-7092	VBD	denotes	were
T9959	7093-7100	VBN	denotes	ignored
T9963	7101-7103	IN	denotes	in
T9964	7104-7108	DT	denotes	this
T9965	7109-7117	NN	denotes	analysis
T9966	7117-7118	.	denotes	.
T9967	7118-7201	sentence	denotes	Neighbor-joining searches were conducted by using the computer program MEGA3 [66].
T9968	7119-7127	NN	denotes	Neighbor
T9970	7127-7128	HYPH	denotes	-
T9969	7128-7135	VBG	denotes	joining
T9971	7136-7144	NNS	denotes	searches
T9973	7145-7149	VBD	denotes	were
T9972	7150-7159	VBN	denotes	conducted
T9974	7160-7162	IN	denotes	by
T9975	7163-7168	VBG	denotes	using
T9976	7169-7172	DT	denotes	the
T9978	7173-7181	NN	denotes	computer
T9977	7182-7189	NN	denotes	program
T9979	7190-7195	NNP	denotes	MEGA3
T9980	7196-7197	-LRB-	denotes	[
T9981	7197-7199	CD	denotes	66
T9982	7199-7200	-RRB-	denotes	]
T9983	7200-7201	.	denotes	.
R5464	T8150	T8151	compound	PCR,analysis
R5465	T8152	T8151	prep	of,analysis
R5466	T8153	T8154	compound	Trip13,cDNA
R5467	T8154	T8152	pobj	cDNA,of
R5468	T8155	T8151	punct	.,analysis
R5469	T8157	T8158	nsubjpass	Trip13,amplified
R5470	T8159	T8158	auxpass	was,amplified
R5471	T8160	T8158	prep	from,amplified
R5472	T8161	T8160	pobj	samples,from
R5473	T8162	T8161	prep	of,samples
R5474	T8163	T8164	poss	Clontech,Panel
R5475	T8164	T8162	pobj	Panel,of
R5476	T8165	T8163	case	's,Clontech
R5477	T8166	T8164	nmod	Mouse,Panel
R5478	T8167	T8168	amod	Multiple,Tissue
R5479	T8168	T8164	compound	Tissue,Panel
R5480	T8169	T8164	compound	cDNA,Panel
R5481	T8170	T8164	nummod	I,Panel
R5482	T8171	T8172	punct	(,http://www.clontech.com
R5483	T8172	T8161	parataxis	http://www.clontech.com,samples
R5484	T8173	T8172	punct	),http://www.clontech.com
R5485	T8174	T8158	punct	", ",amplified
R5486	T8175	T8158	advcl	using,amplified
R5487	T8176	T8177	det	the,primers
R5488	T8177	T8175	dobj	primers,using
R5489	T8178	T8177	amod	following,primers
R5490	T8179	T8177	punct	: ,primers
R5491	T8180	T8181	nummod	5,GCACCATTGCACTTCACATC
R5492	T8181	T8177	appos	GCACCATTGCACTTCACATC,primers
R5493	T8182	T8180	punct	′,5
R5494	T8183	T8181	punct	-,GCACCATTGCACTTCACATC
R5495	T8184	T8181	punct	-,GCACCATTGCACTTCACATC
R5496	T8185	T8181	nummod	3,GCACCATTGCACTTCACATC
R5497	T8186	T8181	punct	′,GCACCATTGCACTTCACATC
R5498	T8187	T8181	punct	(,GCACCATTGCACTTCACATC
R5499	T8188	T8189	compound	TRP3,6F
R5500	T8189	T8181	appos	6F,GCACCATTGCACTTCACATC
R5501	T8190	T8189	punct	-,6F
R5502	T8191	T8181	punct	),GCACCATTGCACTTCACATC
R5503	T8192	T8181	cc	and,GCACCATTGCACTTCACATC
R5504	T8193	T8194	nummod	5,TGACCATCAGACTGTCGAGC
R5505	T8194	T8181	conj	TGACCATCAGACTGTCGAGC,GCACCATTGCACTTCACATC
R5506	T8195	T8193	punct	′,5
R5507	T8196	T8194	punct	-,TGACCATCAGACTGTCGAGC
R5508	T8197	T8194	punct	-,TGACCATCAGACTGTCGAGC
R5509	T8198	T8194	nummod	3,TGACCATCAGACTGTCGAGC
R5510	T8199	T8194	punct	′,TGACCATCAGACTGTCGAGC
R5511	T8200	T8194	punct	(,TGACCATCAGACTGTCGAGC
R5512	T8201	T8202	compound	TRP3,6R
R5513	T8202	T8194	appos	6R,TGACCATCAGACTGTCGAGC
R5514	T8203	T8202	punct	-,6R
R5515	T8204	T8158	punct	),amplified
R5516	T8205	T8158	punct	.,amplified
R5517	T8207	T8208	det	These,primers
R5518	T8208	T8209	nsubj	primers,correspond
R5519	T8210	T8209	prep	to,correspond
R5520	T8211	T8212	nmod	exons,3
R5521	T8212	T8210	pobj	3,to
R5522	T8213	T8212	cc	and,3
R5523	T8214	T8212	conj	6,3
R5524	T8215	T8209	punct	", ",correspond
R5525	T8216	T8209	advmod	respectively,correspond
R5526	T8217	T8209	punct	", ",correspond
R5527	T8218	T8209	cc	and,correspond
R5528	T8219	T8209	conj	amplify,correspond
R5529	T8220	T8221	det	a,product
R5530	T8221	T8219	dobj	product,amplify
R5531	T8222	T8223	nummod	330,bp
R5532	T8223	T8221	compound	bp,product
R5533	T8224	T8223	punct	-,bp
R5534	T8225	T8221	compound	cDNA,product
R5535	T8226	T8209	punct	.,correspond
R5536	T8228	T8229	det	The,cDNAs
R5537	T8229	T8230	nsubjpass	cDNAs,equalized
R5538	T8231	T8229	prep	in,cDNAs
R5539	T8232	T8233	det	this,panel
R5540	T8233	T8231	pobj	panel,in
R5541	T8234	T8230	auxpass	are,equalized
R5542	T8235	T8236	aux	to,allow
R5543	T8236	T8230	advcl	allow,equalized
R5544	T8237	T8238	amod	quantitative,analysis
R5545	T8238	T8236	dobj	analysis,allow
R5546	T8239	T8238	prep	by,analysis
R5547	T8240	T8241	compound	RT,PCR
R5548	T8241	T8239	pobj	PCR,by
R5549	T8242	T8241	punct	-,PCR
R5550	T8243	T8230	punct	.,equalized
R5551	T8309	T8308	prep	of,Generation
R5552	T8310	T8311	npadvmod	Trip13,deficient
R5553	T8311	T8313	amod	deficient,mice
R5554	T8312	T8311	punct	-,deficient
R5555	T8313	T8309	pobj	mice,of
R5556	T8314	T8308	punct	.,Generation
R5557	T8316	T8317	det	The,RRB047
R5558	T8317	T8323	nsubjpass	RRB047,obtained
R5559	T8318	T8317	nmod	mouse,RRB047
R5560	T8319	T8317	amod	embryonic,RRB047
R5561	T8320	T8321	compound	stem,cell
R5562	T8321	T8317	compound	cell,RRB047
R5563	T8322	T8317	compound	line,RRB047
R5564	T8324	T8317	punct	(,RRB047
R5565	T8325	T8326	nmod	strain,Ola
R5566	T8326	T8317	appos	Ola,RRB047
R5567	T8327	T8326	nummod	129,Ola
R5568	T8328	T8326	punct	/,Ola
R5569	T8329	T8317	punct	),RRB047
R5570	T8330	T8317	acl	containing,RRB047
R5571	T8331	T8332	det	a,insertion
R5572	T8332	T8330	dobj	insertion,containing
R5573	T8333	T8334	compound	gene,trap
R5574	T8334	T8332	compound	trap,insertion
R5575	T8335	T8330	prep	in,containing
R5576	T8336	T8335	pobj	Trip13,in
R5577	T8337	T8323	auxpass	was,obtained
R5578	T8338	T8323	prep	from,obtained
R5579	T8339	T8338	pobj	BayGenomics,from
R5580	T8340	T8341	punct	(,http://www.baygenomics.ucsf.edu/
R5581	T8341	T8339	parataxis	http://www.baygenomics.ucsf.edu/,BayGenomics
R5582	T8342	T8341	punct	),http://www.baygenomics.ucsf.edu/
R5583	T8343	T8323	punct	.,obtained
R5584	T8345	T8346	det	The,vector
R5585	T8346	T8350	nsubjpass	vector,designed
R5586	T8347	T8346	nmod	gene,vector
R5587	T8348	T8347	punct	-,gene
R5588	T8349	T8347	amod	trapping,gene
R5589	T8351	T8346	acl	used,vector
R5590	T8352	T8353	aux	to,create
R5591	T8353	T8351	advcl	create,used
R5592	T8354	T8355	det	this,line
R5593	T8355	T8353	dobj	line,create
R5594	T8356	T8346	punct	", ",vector
R5595	T8357	T8346	appos	pGT1lxf,vector
R5596	T8358	T8350	punct	", ",designed
R5597	T8359	T8350	auxpass	was,designed
R5598	T8360	T8361	aux	to,create
R5599	T8361	T8350	advcl	create,designed
R5600	T8362	T8363	det	an,fusion
R5601	T8363	T8361	dobj	fusion,create
R5602	T8364	T8363	nmod	in,fusion
R5603	T8365	T8364	punct	-,in
R5604	T8366	T8364	pobj	frame,in
R5605	T8367	T8363	prep	between,fusion
R5606	T8368	T8369	det	the,exons
R5607	T8369	T8367	pobj	exons,between
R5608	T8370	T8369	nummod	5,exons
R5609	T8371	T8370	punct	′,5
R5610	T8372	T8369	prep	of,exons
R5611	T8373	T8374	det	the,gene
R5612	T8374	T8372	pobj	gene,of
R5613	T8375	T8374	amod	trapped,gene
R5614	T8376	T8374	cc	and,gene
R5615	T8377	T8378	det	a,reporter
R5616	T8378	T8374	conj	reporter,gene
R5617	T8379	T8378	punct	", ",reporter
R5618	T8380	T8378	appos	βgeo,reporter
R5619	T8381	T8380	punct	(,βgeo
R5620	T8382	T8383	det	a,fusion
R5621	T8383	T8380	appos	fusion,βgeo
R5622	T8384	T8383	prep	of,fusion
R5623	T8385	T8386	compound	β,galactosidase
R5624	T8386	T8384	pobj	galactosidase,of
R5625	T8387	T8386	punct	-,galactosidase
R5626	T8388	T8386	cc	and,galactosidase
R5627	T8389	T8390	compound	neomycin,phosphotransferase
R5628	T8390	T8386	conj	phosphotransferase,galactosidase
R5629	T8391	T8390	nummod	II,phosphotransferase
R5630	T8392	T8350	punct	),designed
R5631	T8393	T8350	punct	.,designed
R5632	T8395	T8396	det	The,locus
R5633	T8396	T8400	nsubj	locus,creates
R5634	T8397	T8398	npadvmod	gene,trapped
R5635	T8398	T8396	amod	trapped,locus
R5636	T8399	T8398	punct	-,trapped
R5637	T8401	T8402	det	a,transcript
R5638	T8402	T8400	dobj	transcript,creates
R5639	T8403	T8402	compound	fusion,transcript
R5640	T8404	T8402	acl	containing,transcript
R5641	T8405	T8406	nmod	exons,3
R5642	T8406	T8404	dobj	3,containing
R5643	T8407	T8406	nummod	1,3
R5644	T8408	T8406	punct	–,3
R5645	T8409	T8406	prep	of,3
R5646	T8410	T8409	pobj	Trip13,of
R5647	T8411	T8410	cc	and,Trip13
R5648	T8412	T8410	conj	βgeo,Trip13
R5649	T8413	T8400	punct	.,creates
R5650	T8415	T8416	aux	To,identify
R5651	T8416	T8417	advcl	identify,performed
R5652	T8418	T8419	det	the,site
R5653	T8419	T8416	dobj	site,identify
R5654	T8420	T8419	amod	exact,site
R5655	T8421	T8419	compound	insertion,site
R5656	T8422	T8419	prep	within,site
R5657	T8423	T8422	pobj	intron,within
R5658	T8424	T8423	nummod	3,intron
R5659	T8425	T8417	punct	", ",performed
R5660	T8426	T8417	nsubjpass	PCR,performed
R5661	T8427	T8417	auxpass	was,performed
R5662	T8428	T8417	advcl	using,performed
R5663	T8429	T8430	nummod	one,primer
R5664	T8430	T8428	dobj	primer,using
R5665	T8431	T8430	prep	within,primer
R5666	T8432	T8433	det	the,vector
R5667	T8433	T8431	pobj	vector,within
R5668	T8434	T8435	compound	gene,trap
R5669	T8435	T8433	compound	trap,vector
R5670	T8436	T8430	punct	", ",primer
R5671	T8437	T8430	cc	and,primer
R5672	T8438	T8439	det	the,primer
R5673	T8439	T8430	conj	primer,primer
R5674	T8440	T8439	amod	other,primer
R5675	T8441	T8439	prep	at,primer
R5676	T8442	T8443	amod	various,positions
R5677	T8443	T8441	pobj	positions,at
R5678	T8444	T8443	prep	in,positions
R5679	T8445	T8444	pobj	intron,in
R5680	T8446	T8445	nummod	3,intron
R5681	T8447	T8443	acl	pointing,positions
R5682	T8448	T8447	prep	towards,pointing
R5683	T8449	T8450	det	the,end
R5684	T8450	T8448	pobj	end,towards
R5685	T8451	T8450	nummod	3,end
R5686	T8452	T8451	punct	′,3
R5687	T8453	T8450	prep	of,end
R5688	T8454	T8455	det	the,gene
R5689	T8455	T8453	pobj	gene,of
R5690	T8456	T8417	punct	.,performed
R5691	T8458	T8459	nsubjpass	Product,sequenced
R5692	T8460	T8458	prep	from,Product
R5693	T8461	T8462	det	a,reaction
R5694	T8462	T8460	pobj	reaction,from
R5695	T8463	T8462	amod	productive,reaction
R5696	T8464	T8459	auxpass	was,sequenced
R5697	T8465	T8459	punct	", ",sequenced
R5698	T8466	T8459	advcl	revealing,sequenced
R5699	T8467	T8468	mark	that,was
R5700	T8468	T8466	ccomp	was,revealing
R5701	T8469	T8470	det	the,site
R5702	T8470	T8468	nsubj	site,was
R5703	T8471	T8470	compound	insertion,site
R5704	T8472	T8473	nummod	445,bp
R5705	T8473	T8474	npadvmod	bp,into
R5706	T8474	T8468	prep	into,was
R5707	T8475	T8474	pobj	intron,into
R5708	T8476	T8475	nummod	3,intron
R5709	T8477	T8459	punct	.,sequenced
R5710	T8522	T8521	prep	of,Genotyping
R5711	T8523	T8522	pobj	mice,of
R5712	T8524	T8521	punct	.,Genotyping
R5713	T8526	T8527	nummod	Three,primers
R5714	T8527	T8528	nsubjpass	primers,used
R5715	T8529	T8528	auxpass	were,used
R5716	T8530	T8531	aux	to,distinguish
R5717	T8531	T8528	advcl	distinguish,used
R5718	T8532	T8533	amod	wild,type
R5719	T8533	T8535	nmod	type,alleles
R5720	T8534	T8533	punct	-,type
R5721	T8535	T8531	dobj	alleles,distinguish
R5722	T8536	T8533	cc	and,type
R5723	T8537	T8533	conj	mutant,type
R5724	T8538	T8535	prep	of,alleles
R5725	T8539	T8538	pobj	Trip13,of
R5726	T8540	T8535	punct	: ,alleles
R5727	T8541	T8535	appos	primer,alleles
R5728	T8542	T8541	nummod	1,primer
R5729	T8543	T8541	punct	", ",primer
R5730	T8544	T8545	nummod	5,CGTCGCTCCATTGCTTTGTGC
R5731	T8545	T8541	appos	CGTCGCTCCATTGCTTTGTGC,primer
R5732	T8546	T8544	punct	′,5
R5733	T8547	T8545	punct	-,CGTCGCTCCATTGCTTTGTGC
R5734	T8548	T8545	punct	-,CGTCGCTCCATTGCTTTGTGC
R5735	T8549	T8545	nummod	3,CGTCGCTCCATTGCTTTGTGC
R5736	T8550	T8545	punct	′,CGTCGCTCCATTGCTTTGTGC
R5737	T8551	T8541	punct	;,primer
R5738	T8552	T8541	conj	primer,primer
R5739	T8553	T8552	nummod	2,primer
R5740	T8554	T8552	punct	", ",primer
R5741	T8555	T8556	nummod	5,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5742	T8556	T8552	appos	AGTAGTGGTACACTGTATTTTTGCTTTCATTGA,primer
R5743	T8557	T8555	punct	′,5
R5744	T8558	T8556	punct	-,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5745	T8559	T8556	punct	-,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5746	T8560	T8556	nummod	3,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5747	T8561	T8556	punct	′,AGTAGTGGTACACTGTATTTTTGCTTTCATTGA
R5748	T8562	T8552	punct	;,primer
R5749	T8563	T8552	cc	and,primer
R5750	T8564	T8552	conj	primer,primer
R5751	T8565	T8564	nummod	3,primer
R5752	T8566	T8564	punct	", ",primer
R5753	T8567	T8568	nummod	5,GTAGATCCCGGCGCTCTTACCAA
R5754	T8568	T8564	appos	GTAGATCCCGGCGCTCTTACCAA,primer
R5755	T8569	T8567	punct	′,5
R5756	T8570	T8568	punct	-,GTAGATCCCGGCGCTCTTACCAA
R5757	T8571	T8568	punct	-,GTAGATCCCGGCGCTCTTACCAA
R5758	T8572	T8568	nummod	3,GTAGATCCCGGCGCTCTTACCAA
R5759	T8573	T8568	punct	′,GTAGATCCCGGCGCTCTTACCAA
R5760	T8574	T8528	punct	.,used
R5761	T8576	T8577	nmod	Primers,1
R5762	T8577	T8578	nsubjpass	1,located
R5763	T8579	T8577	cc	and,1
R5764	T8580	T8577	conj	2,1
R5765	T8581	T8578	auxpass	are,located
R5766	T8582	T8578	advmod	upstream,located
R5767	T8583	T8582	cc	and,upstream
R5768	T8584	T8582	conj	downstream,upstream
R5769	T8585	T8584	punct	", ",downstream
R5770	T8586	T8584	conj	respectively,downstream
R5771	T8587	T8586	punct	", ",respectively
R5772	T8588	T8586	conj	of,respectively
R5773	T8589	T8590	det	the,insertion
R5774	T8590	T8588	pobj	insertion,of
R5775	T8591	T8592	compound	gene,trap
R5776	T8592	T8590	compound	trap,insertion
R5777	T8593	T8590	prep	within,insertion
R5778	T8594	T8595	det	the,intron
R5779	T8595	T8593	pobj	intron,within
R5780	T8596	T8595	nummod	3,intron
R5781	T8597	T8578	punct	.,located
R5782	T8599	T8600	nsubj	Primer,corresponds
R5783	T8601	T8599	nummod	3,Primer
R5784	T8602	T8600	prep	to,corresponds
R5785	T8603	T8604	compound	pGTlxf,sequence
R5786	T8604	T8602	pobj	sequence,to
R5787	T8605	T8600	punct	.,corresponds
R5788	T8607	T8608	nmod	Primers,1
R5789	T8608	T8609	nsubj	1,amplify
R5790	T8609	T8612	ccomp	amplify,amplify
R5791	T8610	T8608	cc	and,1
R5792	T8611	T8608	conj	2,1
R5793	T8613	T8614	det	a,band
R5794	T8614	T8609	dobj	band,amplify
R5795	T8615	T8616	nummod	700,bp
R5796	T8616	T8614	compound	bp,band
R5797	T8617	T8616	punct	-,bp
R5798	T8618	T8614	prep	from,band
R5799	T8619	T8620	det	a,allele
R5800	T8620	T8618	pobj	allele,from
R5801	T8621	T8622	amod	wild,type
R5802	T8622	T8620	compound	type,allele
R5803	T8623	T8622	punct	-,type
R5804	T8624	T8612	punct	;,amplify
R5805	T8625	T8626	nmod	primers,1
R5806	T8626	T8612	nsubj	1,amplify
R5807	T8627	T8626	cc	and,1
R5808	T8628	T8626	conj	3,1
R5809	T8629	T8630	det	a,fragment
R5810	T8630	T8612	dobj	fragment,amplify
R5811	T8631	T8632	nummod	540,bp
R5812	T8632	T8630	compound	bp,fragment
R5813	T8633	T8632	punct	-,bp
R5814	T8634	T8630	prep	from,fragment
R5815	T8635	T8636	det	a,allele
R5816	T8636	T8634	pobj	allele,from
R5817	T8637	T8636	compound	mutant,allele
R5818	T8638	T8612	punct	.,amplify
R5819	T8640	T8641	amod	Separate,reactions
R5820	T8641	T8642	nsubjpass	reactions,used
R5821	T8643	T8642	auxpass	were,used
R5822	T8644	T8645	aux	to,assay
R5823	T8645	T8642	advcl	assay,used
R5824	T8646	T8647	det	the,presence
R5825	T8647	T8645	dobj	presence,assay
R5826	T8648	T8647	cc	or,presence
R5827	T8649	T8647	conj	absence,presence
R5828	T8650	T8647	prep	of,presence
R5829	T8651	T8652	det	each,amplicon
R5830	T8652	T8650	pobj	amplicon,of
R5831	T8653	T8645	prep	from,assay
R5832	T8654	T8655	det	a,sample
R5833	T8655	T8653	pobj	sample,from
R5834	T8656	T8655	compound	DNA,sample
R5835	T8657	T8642	punct	.,used
R5836	T8659	T8660	det	The,conditions
R5837	T8660	T8662	nsubj	conditions,were
R5838	T8661	T8660	compound	cycling,conditions
R5839	T8663	T8662	punct	: ,were
R5840	T8664	T8665	nummod	94,°C
R5841	T8665	T8662	attr	°C,were
R5842	T8666	T8667	nummod	2,min
R5843	T8667	T8665	npadvmod	min,°C
R5844	T8668	T8665	punct	;,°C
R5845	T8669	T8670	nummod	35,cycles
R5846	T8670	T8665	conj	cycles,°C
R5847	T8671	T8670	prep	of,cycles
R5848	T8672	T8673	nummod	94,°C
R5849	T8673	T8671	pobj	°C,of
R5850	T8674	T8675	nummod	30,s
R5851	T8675	T8670	npadvmod	s,cycles
R5852	T8676	T8670	punct	", ",cycles
R5853	T8677	T8678	nummod	57,°C
R5854	T8678	T8670	conj	°C,cycles
R5855	T8679	T8680	nummod	45,s
R5856	T8680	T8678	npadvmod	s,°C
R5857	T8681	T8678	punct	", ",°C
R5858	T8682	T8678	cc	and,°C
R5859	T8683	T8684	nummod	72,°C
R5860	T8684	T8678	conj	°C,°C
R5861	T8685	T8686	nummod	50,s
R5862	T8686	T8684	npadvmod	s,°C
R5863	T8687	T8670	punct	;,cycles
R5864	T8688	T8670	cc	and,cycles
R5865	T8689	T8690	nummod	72,°C
R5866	T8690	T8670	conj	°C,cycles
R5867	T8691	T8692	nummod	2,min
R5868	T8692	T8690	npadvmod	min,°C
R5869	T8693	T8662	punct	.,were
R5870	T8717	T8718	compound	RT,PCR
R5871	T8719	T8718	punct	-,PCR
R5872	T8720	T8718	punct	.,PCR
R5873	T8722	T8723	amod	Total,RNA
R5874	T8723	T8724	nsubjpass	RNA,isolated
R5875	T8725	T8724	auxpass	was,isolated
R5876	T8726	T8724	prep	from,isolated
R5877	T8727	T8728	amod	adult,testes
R5878	T8728	T8726	pobj	testes,from
R5879	T8729	T8724	prep	with,isolated
R5880	T8730	T8731	det	the,Kit
R5881	T8731	T8729	pobj	Kit,with
R5882	T8732	T8731	compound	RNeasy,Kit
R5883	T8733	T8731	compound	Mini,Kit
R5884	T8734	T8735	punct	(,Qiagen
R5885	T8735	T8731	parataxis	Qiagen,Kit
R5886	T8736	T8735	punct	", ",Qiagen
R5887	T8737	T8735	npadvmod	http://www.qiagen.com,Qiagen
R5888	T8738	T8735	punct	),Qiagen
R5889	T8739	T8724	punct	", ",isolated
R5890	T8740	T8724	cc	and,isolated
R5891	T8741	T8742	nummod	4.0,μg
R5892	T8742	T8743	nsubj	μg,primed
R5893	T8743	T8724	conj	primed,isolated
R5894	T8744	T8743	aux	was,primed
R5895	T8745	T8746	compound	oligo,dT
R5896	T8746	T8743	npadvmod	dT,primed
R5897	T8747	T8743	punct	–,primed
R5898	T8748	T8743	cc	and,primed
R5899	T8749	T8750	advmod	reverse,transcribed
R5900	T8750	T8743	conj	transcribed,primed
R5901	T8751	T8750	punct	-,transcribed
R5902	T8752	T8743	prep	with,primed
R5903	T8753	T8752	pobj	Superscript,with
R5904	T8754	T8753	nummod	II,Superscript
R5905	T8755	T8756	punct	(,Stratagene
R5906	T8756	T8753	parataxis	Stratagene,Superscript
R5907	T8757	T8756	punct	", ",Stratagene
R5908	T8758	T8756	npadvmod	http://www.stratagene.com,Stratagene
R5909	T8759	T8756	punct	),Stratagene
R5910	T8760	T8743	punct	.,primed
R5911	T8762	T8763	det	The,sequence
R5912	T8763	T8769	nsubjpass	sequence,amplified
R5913	T8764	T8763	amod	entire,sequence
R5914	T8765	T8763	nmod	Trip13,sequence
R5915	T8766	T8767	npadvmod	protein,coding
R5916	T8767	T8763	amod	coding,sequence
R5917	T8768	T8767	punct	-,coding
R5918	T8770	T8769	auxpass	was,amplified
R5919	T8771	T8769	prep	with,amplified
R5920	T8772	T8773	nmod	primers,ATGGACGAGGCGGTG
R5921	T8773	T8771	pobj	ATGGACGAGGCGGTG,with
R5922	T8774	T8773	nummod	5,ATGGACGAGGCGGTG
R5923	T8775	T8774	punct	′,5
R5924	T8776	T8773	punct	-,ATGGACGAGGCGGTG
R5925	T8777	T8773	punct	-,ATGGACGAGGCGGTG
R5926	T8778	T8773	nummod	3,ATGGACGAGGCGGTG
R5927	T8779	T8773	punct	′,ATGGACGAGGCGGTG
R5928	T8780	T8773	cc	and,ATGGACGAGGCGGTG
R5929	T8781	T8782	nummod	5,TCAAACATAAGCTGAAAGTT
R5930	T8782	T8773	conj	TCAAACATAAGCTGAAAGTT,ATGGACGAGGCGGTG
R5931	T8783	T8781	punct	′,5
R5932	T8784	T8782	punct	-,TCAAACATAAGCTGAAAGTT
R5933	T8785	T8782	punct	-,TCAAACATAAGCTGAAAGTT
R5934	T8786	T8782	nummod	3,TCAAACATAAGCTGAAAGTT
R5935	T8787	T8782	punct	′,TCAAACATAAGCTGAAAGTT
R5936	T8788	T8769	punct	.,amplified
R5937	T8790	T8791	det	The,conditions
R5938	T8791	T8793	nsubj	conditions,were
R5939	T8792	T8791	compound	cycling,conditions
R5940	T8794	T8793	punct	: ,were
R5941	T8795	T8796	nummod	94,°C
R5942	T8796	T8793	attr	°C,were
R5943	T8797	T8798	nummod	2,min
R5944	T8798	T8796	npadvmod	min,°C
R5945	T8799	T8796	punct	;,°C
R5946	T8800	T8801	nummod	94,°C
R5947	T8801	T8796	conj	°C,°C
R5948	T8802	T8803	nummod	30,s
R5949	T8803	T8801	npadvmod	s,°C
R5950	T8804	T8801	punct	", ",°C
R5951	T8805	T8806	nummod	55,°C
R5952	T8806	T8801	conj	°C,°C
R5953	T8807	T8808	nummod	45,s
R5954	T8808	T8806	npadvmod	s,°C
R5955	T8809	T8806	punct	", ",°C
R5956	T8810	T8806	cc	and,°C
R5957	T8811	T8812	nummod	72,°C
R5958	T8812	T8806	conj	°C,°C
R5959	T8813	T8814	nummod	80,s
R5960	T8814	T8812	npadvmod	s,°C
R5961	T8815	T8812	prep	for,°C
R5962	T8816	T8817	nummod	35,cycles
R5963	T8817	T8815	pobj	cycles,for
R5964	T8818	T8801	punct	;,°C
R5965	T8819	T8801	cc	and,°C
R5966	T8820	T8821	nummod	72,°C
R5967	T8821	T8801	conj	°C,°C
R5968	T8822	T8823	nummod	2,min
R5969	T8823	T8821	npadvmod	min,°C
R5970	T8824	T8793	punct	.,were
R5971	T8826	T8827	det	The,primers
R5972	T8827	T8828	nsubj	primers,were
R5973	T8829	T8827	prep	for,primers
R5974	T8830	T8829	pcomp	amplifying,for
R5975	T8831	T8832	det	the,sequence
R5976	T8832	T8830	dobj	sequence,amplifying
R5977	T8833	T8832	compound	Med31,sequence
R5978	T8834	T8832	compound	coding,sequence
R5979	T8835	T8830	prep	as,amplifying
R5980	T8836	T8835	pobj	control,as
R5981	T8837	T8828	punct	: ,were
R5982	T8838	T8839	nummod	5,ATGGCCGCGGCCGTCGCTATGG
R5983	T8839	T8828	attr	ATGGCCGCGGCCGTCGCTATGG,were
R5984	T8840	T8838	punct	′,5
R5985	T8841	T8839	punct	-,ATGGCCGCGGCCGTCGCTATGG
R5986	T8842	T8839	punct	-,ATGGCCGCGGCCGTCGCTATGG
R5987	T8843	T8839	nummod	3,ATGGCCGCGGCCGTCGCTATGG
R5988	T8844	T8839	punct	′,ATGGCCGCGGCCGTCGCTATGG
R5989	T8845	T8839	cc	and,ATGGCCGCGGCCGTCGCTATGG
R5990	T8846	T8847	nummod	5,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC
R5991	T8847	T8839	conj	TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC,ATGGCCGCGGCCGTCGCTATGG
R5992	T8848	T8846	punct	′,5
R5993	T8849	T8847	punct	-,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC
R5994	T8850	T8847	punct	-,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC
R5995	T8851	T8847	nummod	3,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC
R5996	T8852	T8847	punct	′,TCATTTCCCTGCTGTGTTATTCTGCTGCTGCTGC
R5997	T8853	T8828	punct	.,were
R5998	T8855	T8856	det	The,conditions
R5999	T8856	T8858	nsubj	conditions,were
R6000	T8857	T8856	compound	cycling,conditions
R6001	T8859	T8858	punct	: ,were
R6002	T8860	T8861	nummod	94,°C
R6003	T8861	T8858	attr	°C,were
R6004	T8862	T8863	nummod	2,min
R6005	T8863	T8861	npadvmod	min,°C
R6006	T8864	T8861	punct	;,°C
R6007	T8865	T8866	nummod	94,°C
R6008	T8866	T8861	conj	°C,°C
R6009	T8867	T8868	nummod	30,s
R6010	T8868	T8866	npadvmod	s,°C
R6011	T8869	T8866	punct	", ",°C
R6012	T8870	T8871	nummod	55,°C
R6013	T8871	T8866	conj	°C,°C
R6014	T8872	T8873	nummod	30,s
R6015	T8873	T8871	npadvmod	s,°C
R6016	T8874	T8871	punct	", ",°C
R6017	T8875	T8871	cc	and,°C
R6018	T8876	T8877	nummod	72,°C
R6019	T8877	T8871	conj	°C,°C
R6020	T8878	T8879	nummod	35,s
R6021	T8879	T8877	npadvmod	s,°C
R6022	T8880	T8877	prep	for,°C
R6023	T8881	T8882	nummod	35,cycles
R6024	T8882	T8880	pobj	cycles,for
R6025	T8883	T8866	punct	;,°C
R6026	T8884	T8866	cc	and,°C
R6027	T8885	T8886	nummod	72,°C
R6028	T8886	T8866	conj	°C,°C
R6029	T8887	T8888	nummod	2,min
R6030	T8888	T8886	npadvmod	min,°C
R6031	T8889	T8858	punct	.,were
R6034	T8919	T8918	cc	and,Development
R6035	T8920	T8918	conj	purification,Development
R6036	T8921	T8918	prep	of,Development
R6037	T8922	T8923	compound	chicken,antibodies
R6038	T8923	T8921	pobj	antibodies,of
R6039	T8924	T8918	punct	.,Development
R6040	T8926	T8927	det	A,peptide
R6041	T8927	T8928	nsubjpass	peptide,conjugated
R6042	T8929	T8927	acl	corresponding,peptide
R6043	T8930	T8929	prep	to,corresponding
R6044	T8931	T8932	nmod	amino,25
R6045	T8932	T8930	pobj	25,to
R6046	T8933	T8932	nmod	acids,25
R6047	T8934	T8935	punct	–,40
R6048	T8935	T8932	prep	40,25
R6049	T8936	T8932	prep	of,25
R6050	T8937	T8936	pobj	TRIP13,of
R6051	T8938	T8927	punct	", ",peptide
R6052	T8939	T8927	appos	VLQRSGSTAKKEDIK,peptide
R6053	T8940	T8928	punct	", ",conjugated
R6054	T8941	T8928	auxpass	was,conjugated
R6055	T8942	T8928	prep	to,conjugated
R6056	T8943	T8942	pobj	KLH,to
R6057	T8944	T8928	cc	and,conjugated
R6058	T8945	T8928	conj	used,conjugated
R6059	T8946	T8947	aux	to,immunize
R6060	T8947	T8945	advcl	immunize,used
R6061	T8948	T8947	dobj	chickens,immunize
R6062	T8949	T8950	punct	(,done
R6063	T8950	T8945	parataxis	done,used
R6064	T8951	T8950	prep	by,done
R6065	T8952	T8953	compound	Sigma,Genosys
R6066	T8953	T8951	pobj	Genosys,by
R6067	T8954	T8953	punct	", ",Genosys
R6068	T8955	T8953	npadvmod	http://www.sigmaaldrich.com,Genosys
R6069	T8956	T8950	punct	),done
R6070	T8957	T8928	punct	.,conjugated
R6071	T8959	T8960	amod	Polyclonal,IgY
R6072	T8960	T8961	nsubjpass	IgY,isolated
R6073	T8962	T8961	auxpass	was,isolated
R6074	T8963	T8961	prep	from,isolated
R6075	T8964	T8963	pobj	eggs,from
R6076	T8965	T8961	prep	with,isolated
R6077	T8966	T8967	det	the,kit
R6078	T8967	T8965	pobj	kit,with
R6079	T8968	T8969	compound	Eggcellent,Chicken
R6080	T8969	T8967	compound	Chicken,kit
R6081	T8970	T8967	compound	IgY,kit
R6082	T8971	T8967	compound	Purification,kit
R6083	T8972	T8973	punct	(,Pierce
R6084	T8973	T8967	parataxis	Pierce,kit
R6085	T8974	T8973	punct	", ",Pierce
R6086	T8975	T8973	npadvmod	http://www.piercenet.com,Pierce
R6087	T8976	T8973	punct	),Pierce
R6088	T8977	T8961	punct	.,isolated
R6089	T8979	T8980	compound	IgY,antibodies
R6090	T8980	T8981	nsubjpass	antibodies,purified
R6091	T8982	T8981	auxpass	were,purified
R6092	T8983	T8981	advmod	then,purified
R6093	T8984	T8981	dep	affinity,purified
R6094	T8985	T8981	advcl	using,purified
R6095	T8986	T8987	det	the,peptide
R6096	T8987	T8985	dobj	peptide,using
R6097	T8988	T8987	amod	immunizing,peptide
R6098	T8989	T8987	amod	synthetic,peptide
R6099	T8990	T8981	punct	.,purified
R6100	T9024	T9025	compound	Western,blotting
R6101	T9026	T9025	punct	.,blotting
R6102	T9028	T9029	nummod	50,μg
R6103	T9029	T9030	nsubjpass	μg,separated
R6104	T9031	T9029	prep	of,μg
R6105	T9032	T9033	compound	testis,extract
R6106	T9033	T9031	pobj	extract,of
R6107	T9034	T9029	prep	in,μg
R6108	T9035	T9036	compound	RIPA,buffer
R6109	T9036	T9034	pobj	buffer,in
R6110	T9037	T9030	auxpass	was,separated
R6111	T9038	T9030	agent	by,separated
R6112	T9039	T9040	nummod	8,%
R6113	T9040	T9041	compound	%,PAGE
R6114	T9041	T9038	pobj	PAGE,by
R6115	T9042	T9041	compound	SDS,PAGE
R6116	T9043	T9041	punct	-,PAGE
R6117	T9044	T9030	cc	and,separated
R6118	T9045	T9030	conj	electrotransferred,separated
R6119	T9046	T9045	prep	onto,electrotransferred
R6120	T9047	T9048	det	a,membrane
R6121	T9048	T9046	pobj	membrane,onto
R6122	T9049	T9048	compound	Pure,membrane
R6123	T9050	T9048	compound	Nitrocellulose,membrane
R6124	T9051	T9052	punct	(,Rad
R6125	T9052	T9048	parataxis	Rad,membrane
R6126	T9053	T9052	compound	Bio,Rad
R6127	T9054	T9052	punct	-,Rad
R6128	T9055	T9052	punct	", ",Rad
R6129	T9056	T9052	npadvmod	http://www.biorad.com,Rad
R6130	T9057	T9052	punct	),Rad
R6131	T9058	T9030	punct	.,separated
R6132	T9060	T9061	det	The,membrane
R6133	T9061	T9062	nsubjpass	membrane,incubated
R6134	T9063	T9062	auxpass	was,incubated
R6135	T9064	T9062	prep	with,incubated
R6136	T9065	T9066	det	a,antibody
R6137	T9066	T9064	pobj	antibody,with
R6138	T9067	T9068	amod	polyclonal,rabbit
R6139	T9068	T9066	nmod	rabbit,antibody
R6140	T9069	T9066	amod	anti-human,antibody
R6141	T9070	T9066	compound	TRIP13,antibody
R6142	T9071	T9072	punct	(,Genway
R6143	T9072	T9066	parataxis	Genway,antibody
R6144	T9073	T9074	nummod	18,42687
R6145	T9074	T9072	dep	42687,Genway
R6146	T9075	T9074	punct	-,42687
R6147	T9076	T9074	nummod	003,42687
R6148	T9077	T9074	punct	-,42687
R6149	T9078	T9072	punct	;,Genway
R6150	T9079	T9072	punct	", ",Genway
R6151	T9080	T9072	npadvmod	http://www.genwaybio.com,Genway
R6152	T9081	T9072	punct	),Genway
R6153	T9082	T9062	punct	.,incubated
R6154	T9084	T9085	prep	According,was
R6155	T9086	T9084	prep	to,According
R6156	T9087	T9088	det	the,manufacturer
R6157	T9088	T9086	pobj	manufacturer,to
R6158	T9089	T9085	punct	", ",was
R6159	T9090	T9091	det	the,immunogen
R6160	T9091	T9085	nsubj	immunogen,was
R6161	T9092	T9093	det	a,peptide
R6162	T9093	T9085	attr	peptide,was
R6163	T9094	T9093	amod	synthetic,peptide
R6164	T9095	T9093	acl	embedded,peptide
R6165	T9096	T9095	prep	in,embedded
R6166	T9097	T9096	pobj	sequence,in
R6167	T9098	T9099	nsubj	we,deduced
R6168	T9099	T9097	advcl	deduced,sequence
R6169	T9100	T9101	aux	to,correspond
R6170	T9101	T9099	xcomp	correspond,deduced
R6171	T9102	T9101	prep	to,correspond
R6172	T9103	T9102	pobj	exon,to
R6173	T9104	T9103	nummod	3,exon
R6174	T9105	T9085	punct	.,was
R6175	T9107	T9108	nsubjpass	Binding,detected
R6176	T9109	T9108	auxpass	was,detected
R6177	T9110	T9108	prep	by,detected
R6178	T9111	T9112	compound	chemiluminescence,kit
R6179	T9112	T9110	pobj	kit,by
R6180	T9113	T9112	compound	ECL,kit
R6181	T9114	T9115	punct	(,Pierce
R6182	T9115	T9112	parataxis	Pierce,kit
R6183	T9116	T9115	punct	),Pierce
R6184	T9117	T9108	advcl	using,detected
R6185	T9118	T9119	det	a,conjugate
R6186	T9119	T9117	dobj	conjugate,using
R6187	T9120	T9119	nmod	rabbit,conjugate
R6188	T9121	T9119	amod	anti-chicken,conjugate
R6189	T9122	T9119	compound	IgG,conjugate
R6190	T9123	T9124	compound	horseradish,peroxidase
R6191	T9124	T9119	compound	peroxidase,conjugate
R6192	T9125	T9126	punct	(,Pierce
R6193	T9126	T9119	parataxis	Pierce,conjugate
R6194	T9127	T9126	punct	),Pierce
R6195	T9128	T9108	punct	.,detected
R6196	T9148	T9149	amod	Histological,analyses
R6197	T9150	T9149	punct	.,analyses
R6198	T9152	T9153	nsubjpass	Testes,fixed
R6199	T9154	T9152	cc	or,Testes
R6200	T9155	T9152	conj	ovaries,Testes
R6201	T9156	T9153	auxpass	were,fixed
R6202	T9157	T9153	prep	in,fixed
R6203	T9158	T9157	pobj	Bouin,in
R6204	T9159	T9158	case	's,Bouin
R6205	T9160	T9153	punct	", ",fixed
R6206	T9161	T9153	conj	embedded,fixed
R6207	T9162	T9161	prep	in,embedded
R6208	T9163	T9162	pobj	paraffin,in
R6209	T9164	T9161	punct	", ",embedded
R6210	T9165	T9161	conj	sectioned,embedded
R6211	T9166	T9165	prep	at,sectioned
R6212	T9167	T9168	nummod	6,μm
R6213	T9168	T9166	pobj	μm,at
R6214	T9169	T9165	punct	", ",sectioned
R6215	T9170	T9165	cc	and,sectioned
R6216	T9171	T9165	conj	stained,sectioned
R6217	T9172	T9171	prep	by,stained
R6218	T9173	T9172	pobj	hematoxylin,by
R6219	T9174	T9173	cc	and,hematoxylin
R6220	T9175	T9173	conj	eosin,hematoxylin
R6221	T9176	T9153	punct	.,fixed
R6222	T9178	T9179	compound	Antigen,retrieval
R6223	T9179	T9180	nsubj	retrieval,was
R6224	T9181	T9179	prep	for,retrieval
R6225	T9182	T9181	pobj	immunohistochemistry,for
R6226	T9183	T9182	prep	of,immunohistochemistry
R6227	T9184	T9185	compound	testis,sections
R6228	T9185	T9183	pobj	sections,of
R6229	T9186	T9187	mark	as,described
R6230	T9187	T9180	advcl	described,was
R6231	T9188	T9189	punct	[,60
R6232	T9189	T9180	parataxis	60,was
R6233	T9190	T9189	punct	],60
R6234	T9191	T9180	punct	.,was
R6235	T9193	T9194	nmod	Oocyte,numbers
R6236	T9194	T9197	nsubjpass	numbers,counted
R6237	T9195	T9193	cc	and,Oocyte
R6238	T9196	T9193	conj	follicle,Oocyte
R6239	T9198	T9197	auxpass	were,counted
R6240	T9199	T9200	mark	as,described
R6241	T9200	T9197	advcl	described,counted
R6242	T9201	T9202	punct	[,61
R6243	T9202	T9197	parataxis	61,counted
R6244	T9203	T9202	punct	],61
R6245	T9204	T9197	punct	.,counted
R6246	T9206	T9207	advmod	Only,follicles
R6247	T9207	T9208	nsubjpass	follicles,scored
R6248	T9209	T9207	acl	containing,follicles
R6249	T9210	T9211	det	an,oocyte
R6250	T9211	T9209	dobj	oocyte,containing
R6251	T9212	T9211	prep	with,oocyte
R6252	T9213	T9214	det	a,nucleus
R6253	T9214	T9212	pobj	nucleus,with
R6254	T9215	T9216	advmod	clearly,visible
R6255	T9216	T9214	amod	visible,nucleus
R6256	T9217	T9208	auxpass	were,scored
R6257	T9218	T9208	punct	.,scored
R6260	T9323	T9322	punct	.,Immunocytochemistry
R6261	T9325	T9326	nsubjpass	Immunolabeling,performed
R6262	T9327	T9325	prep	of,Immunolabeling
R6263	T9328	T9329	compound	surface,spermatocytes
R6264	T9329	T9327	pobj	spermatocytes,of
R6265	T9330	T9329	punct	-,spermatocytes
R6266	T9331	T9329	compound	spread,spermatocytes
R6267	T9332	T9329	cc	and,spermatocytes
R6268	T9333	T9329	conj	oocytes,spermatocytes
R6269	T9334	T9326	auxpass	was,performed
R6270	T9335	T9336	mark	as,described
R6271	T9336	T9326	advcl	described,performed
R6272	T9337	T9338	punct	[,62
R6273	T9338	T9326	parataxis	62,performed
R6274	T9339	T9338	nummod	39,62
R6275	T9340	T9338	punct	",",62
R6276	T9341	T9338	punct	],62
R6277	T9342	T9326	punct	.,performed
R6278	T9344	T9345	aux	To,reach
R6279	T9345	T9346	advcl	reach,identified
R6280	T9347	T9345	dobj	conclusions,reach
R6281	T9348	T9347	prep	on,conclusions
R6282	T9349	T9350	det	the,pattern
R6283	T9350	T9348	pobj	pattern,on
R6284	T9351	T9350	prep	of,pattern
R6285	T9352	T9351	pobj	staining,of
R6286	T9353	T9350	prep	for,pattern
R6287	T9354	T9355	amod	various,proteins
R6288	T9355	T9353	pobj	proteins,for
R6289	T9356	T9346	punct	", ",identified
R6290	T9357	T9358	nummod	30,nuclei
R6291	T9358	T9346	nsubjpass	nuclei,identified
R6292	T9359	T9360	punct	(,indicated
R6293	T9360	T9358	parataxis	indicated,nuclei
R6294	T9361	T9360	mark	unless,indicated
R6295	T9362	T9360	advmod	otherwise,indicated
R6296	T9363	T9360	punct	),indicated
R6297	T9364	T9365	advmod	well,spread
R6298	T9365	T9358	amod	spread,nuclei
R6299	T9366	T9365	punct	-,spread
R6300	T9367	T9358	prep	of,nuclei
R6301	T9368	T9369	amod	particular,stages
R6302	T9369	T9367	pobj	stages,of
R6303	T9370	T9369	amod	meiotic,stages
R6304	T9371	T9346	auxpass	were,identified
R6305	T9372	T9346	advmod	first,identified
R6306	T9373	T9346	prep	under,identified
R6307	T9374	T9375	det	the,microscope
R6308	T9375	T9373	pobj	microscope,under
R6309	T9376	T9375	amod	fluorescent,microscope
R6310	T9377	T9346	prep	on,identified
R6311	T9378	T9379	det	the,basis
R6312	T9379	T9377	pobj	basis,on
R6313	T9380	T9379	prep	of,basis
R6314	T9381	T9382	nmod	SYCP3,staining
R6315	T9382	T9380	pobj	staining,of
R6316	T9383	T9381	cc	or,SYCP3
R6317	T9384	T9381	conj	STAG3,SYCP3
R6318	T9385	T9346	punct	", ",identified
R6319	T9386	T9387	advmod	then,imaged
R6320	T9387	T9346	dep	imaged,identified
R6321	T9388	T9387	prep	at,imaged
R6322	T9389	T9390	det	both,wavelengths
R6323	T9390	T9388	pobj	wavelengths,at
R6324	T9391	T9390	amod	appropriate,wavelengths
R6325	T9392	T9393	aux	to,determine
R6326	T9393	T9387	advcl	determine,imaged
R6327	T9394	T9395	det	the,pattern
R6328	T9395	T9393	dobj	pattern,determine
R6329	T9396	T9395	prep	of,pattern
R6330	T9397	T9398	amod	second,proteins
R6331	T9398	T9396	pobj	proteins,of
R6332	T9399	T9393	prep	with,determine
R6333	T9400	T9401	amod	focal,patterns
R6334	T9401	T9399	pobj	patterns,with
R6335	T9402	T9403	amod	such,as
R6336	T9403	T9401	prep	as,patterns
R6337	T9404	T9403	pobj	RAD51,as
R6338	T9405	T9404	cc	or,RAD51
R6339	T9406	T9404	conj	RPA,RAD51
R6340	T9407	T9346	punct	.,identified
R6341	T9409	T9410	mark	Unless,indicated
R6342	T9410	T9412	advcl	indicated,were
R6343	T9411	T9410	advmod	otherwise,indicated
R6344	T9413	T9412	punct	", ",were
R6345	T9414	T9415	det	the,panels
R6346	T9415	T9412	nsubj	panels,were
R6347	T9416	T9415	acl	shown,panels
R6348	T9417	T9416	prep	in,shown
R6349	T9418	T9419	det	the,figures
R6350	T9419	T9417	pobj	figures,in
R6351	T9420	T9421	det	the,patterns
R6352	T9421	T9412	attr	patterns,were
R6353	T9422	T9421	amod	exclusive,patterns
R6354	T9423	T9422	cc	or,exclusive
R6355	T9424	T9422	conj	predominant,exclusive
R6356	T9425	T9421	acl	seen,patterns
R6357	T9426	T9412	punct	.,were
R6358	T9428	T9429	det	The,exception
R6359	T9429	T9430	nsubj	exception,was
R6360	T9431	T9429	prep	for,exception
R6361	T9432	T9433	det	this,approach
R6362	T9433	T9431	pobj	approach,for
R6363	T9434	T9430	prep	in,was
R6364	T9435	T9436	det	the,case
R6365	T9436	T9434	pobj	case,in
R6366	T9437	T9436	prep	of,case
R6367	T9438	T9437	pcomp	staining,of
R6368	T9439	T9438	prep	for,staining
R6369	T9440	T9439	pobj	MLH1,for
R6370	T9441	T9440	cc	or,MLH1
R6371	T9442	T9440	conj	MLH3,MLH1
R6372	T9443	T9440	cc	plus,MLH1
R6373	T9444	T9440	conj	RAD51,MLH1
R6374	T9445	T9444	punct	(,RAD51
R6375	T9446	T9447	prep	in,was
R6376	T9447	T9444	advcl	was,RAD51
R6377	T9448	T9449	det	which,case
R6378	T9449	T9446	pcomp	case,in
R6379	T9450	T9447	nsubj	SYCP3,was
R6380	T9451	T9450	cc	or,SYCP3
R6381	T9452	T9450	conj	STAG3,SYCP3
R6382	T9453	T9447	neg	not,was
R6383	T9454	T9447	acomp	available,was
R6384	T9455	T9456	aux	to,find
R6385	T9456	T9447	advcl	find,was
R6386	T9457	T9458	compound	chromosome,cores
R6387	T9458	T9456	dobj	cores,find
R6388	T9459	T9430	punct	),was
R6389	T9460	T9430	punct	.,was
R6390	T9462	T9463	nsubjpass	Nuclei,identified
R6391	T9464	T9462	prep	in,Nuclei
R6392	T9465	T9466	det	this,situation
R6393	T9466	T9464	pobj	situation,in
R6394	T9467	T9463	auxpass	were,identified
R6395	T9468	T9463	advmod	first,identified
R6396	T9469	T9463	agent	by,identified
R6397	T9470	T9471	nmod	MLH1,foci
R6398	T9471	T9469	pobj	foci,by
R6399	T9472	T9470	punct	/,MLH1
R6400	T9473	T9470	nummod	3,MLH1
R6401	T9474	T9471	amod	clustering,foci
R6402	T9475	T9463	punct	", ",identified
R6403	T9476	T9477	advmod	then,imaged
R6404	T9477	T9463	dep	imaged,identified
R6405	T9478	T9477	prep	for,imaged
R6406	T9479	T9480	det	both,wavelengths
R6407	T9480	T9478	pobj	wavelengths,for
R6408	T9481	T9480	amod	fluorescent,wavelengths
R6409	T9482	T9463	punct	.,identified
R6410	T9484	T9485	amod	Primary,antibodies
R6411	T9485	T9486	nsubj	antibodies,were
R6412	T9487	T9485	acl	used,antibodies
R6413	T9488	T9487	prep	in,used
R6414	T9489	T9490	det	this,study
R6415	T9490	T9488	pobj	study,in
R6416	T9491	T9492	mark	as,follows
R6417	T9492	T9486	advcl	follows,were
R6418	T9493	T9486	punct	: ,were
R6419	T9494	T9486	dep	mouse,were
R6420	T9495	T9494	amod	anti-SCP3,mouse
R6421	T9496	T9497	punct	(,Abcam
R6422	T9497	T9494	parataxis	Abcam,mouse
R6423	T9498	T9497	dep	1,Abcam
R6424	T9499	T9500	punct	:,500
R6425	T9500	T9498	prep	500,1
R6426	T9501	T9497	punct	;,Abcam
R6427	T9502	T9497	punct	", ",Abcam
R6428	T9503	T9497	npadvmod	http://www.abcam.com,Abcam
R6429	T9504	T9497	punct	),Abcam
R6430	T9505	T9494	punct	;,mouse
R6431	T9506	T9507	compound	rabbit,anti-SYCP1
R6432	T9507	T9494	conj	anti-SYCP1,mouse
R6433	T9508	T9509	punct	(,gift
R6434	T9509	T9507	parataxis	gift,anti-SYCP1
R6435	T9510	T9509	dep	1,gift
R6436	T9511	T9512	punct	:,"1,000"
R6437	T9512	T9510	prep	"1,000",1
R6438	T9513	T9509	punct	;,gift
R6439	T9514	T9509	det	a,gift
R6440	T9515	T9509	prep	from,gift
R6441	T9516	T9517	compound	C.,Heyting
R6442	T9517	T9515	pobj	Heyting,from
R6443	T9518	T9509	punct	),gift
R6444	T9519	T9520	punct	[,63
R6445	T9520	T9507	parataxis	63,anti-SYCP1
R6446	T9521	T9520	punct	],63
R6447	T9522	T9507	punct	;,anti-SYCP1
R6448	T9523	T9524	compound	rabbit,anti-REC8
R6449	T9524	T9507	conj	anti-REC8,anti-SYCP1
R6450	T9525	T9526	punct	(,gift
R6451	T9526	T9524	parataxis	gift,anti-REC8
R6452	T9527	T9526	dep	1,gift
R6453	T9528	T9529	punct	:,100
R6454	T9529	T9527	prep	100,1
R6455	T9530	T9526	punct	;,gift
R6456	T9531	T9526	det	a,gift
R6457	T9532	T9526	prep	from,gift
R6458	T9533	T9534	compound	C.,Heyting
R6459	T9534	T9532	pobj	Heyting,from
R6460	T9535	T9526	punct	),gift
R6461	T9536	T9524	punct	;,anti-REC8
R6462	T9537	T9538	compound	rabbit,anti-RAD51
R6463	T9538	T9524	conj	anti-RAD51,anti-REC8
R6464	T9539	T9540	punct	(,Products
R6465	T9540	T9538	parataxis	Products,anti-RAD51
R6466	T9541	T9540	dep	1,Products
R6467	T9542	T9543	punct	:,250
R6468	T9543	T9541	prep	250,1
R6469	T9544	T9540	punct	", ",Products
R6470	T9545	T9546	det	this,antibody
R6471	T9546	T9548	nsubj	antibody,recognizes
R6472	T9547	T9546	amod	polyclonal,antibody
R6473	T9548	T9540	ccomp	recognizes,Products
R6474	T9549	T9550	preconj	both,RAD51
R6475	T9550	T9548	dobj	RAD51,recognizes
R6476	T9551	T9550	cc	and,RAD51
R6477	T9552	T9550	conj	DMC1,RAD51
R6478	T9553	T9540	punct	;,Products
R6479	T9554	T9540	compound	Oncogene,Products
R6480	T9555	T9540	compound	Research,Products
R6481	T9556	T9540	punct	", ",Products
R6482	T9557	T9540	npadvmod	http://www.merckbiosciences.co.uk,Products
R6483	T9558	T9540	punct	),Products
R6484	T9559	T9538	punct	;,anti-RAD51
R6485	T9560	T9561	compound	rabbit,anti-γH2AX
R6486	T9561	T9538	conj	anti-γH2AX,anti-RAD51
R6487	T9562	T9563	punct	(,Biotechnology
R6488	T9563	T9561	parataxis	Biotechnology,anti-γH2AX
R6489	T9564	T9563	dep	1,Biotechnology
R6490	T9565	T9566	punct	:,500
R6491	T9566	T9564	prep	500,1
R6492	T9567	T9563	punct	;,Biotechnology
R6493	T9568	T9563	compound	Upstate,Biotechnology
R6494	T9569	T9563	punct	", ",Biotechnology
R6495	T9570	T9563	npadvmod	http://www.upstate.com/,Biotechnology
R6496	T9571	T9563	punct	),Biotechnology
R6497	T9572	T9561	punct	;,anti-γH2AX
R6498	T9573	T9574	compound	rabbit,anti-STAG3
R6499	T9574	T9561	conj	anti-STAG3,anti-γH2AX
R6500	T9575	T9576	punct	(,gift
R6501	T9576	T9574	parataxis	gift,anti-STAG3
R6502	T9577	T9576	dep	1,gift
R6503	T9578	T9579	punct	:,"1,000"
R6504	T9579	T9577	prep	"1,000",1
R6505	T9580	T9576	punct	;,gift
R6506	T9581	T9576	det	a,gift
R6507	T9582	T9576	prep	from,gift
R6508	T9583	T9584	compound	R.,Jessberger
R6509	T9584	T9582	pobj	Jessberger,from
R6510	T9585	T9576	punct	),gift
R6511	T9586	T9574	punct	;,anti-STAG3
R6512	T9587	T9588	compound	rabbit,anti-MLH3
R6513	T9588	T9574	conj	anti-MLH3,anti-STAG3
R6514	T9589	T9590	punct	(,gift
R6515	T9590	T9588	parataxis	gift,anti-MLH3
R6516	T9591	T9590	dep	1,gift
R6517	T9592	T9593	punct	:,400
R6518	T9593	T9591	prep	400,1
R6519	T9594	T9590	punct	;,gift
R6520	T9595	T9590	det	a,gift
R6521	T9596	T9590	prep	from,gift
R6522	T9597	T9598	compound	P.,Cohen
R6523	T9598	T9596	pobj	Cohen,from
R6524	T9599	T9590	punct	),gift
R6525	T9600	T9588	punct	;,anti-MLH3
R6526	T9601	T9602	amod	mouse,MLH1
R6527	T9602	T9588	conj	MLH1,anti-MLH3
R6528	T9603	T9602	punct	-,MLH1
R6529	T9604	T9602	amod	anti-human,MLH1
R6530	T9605	T9606	punct	(,Biosciences
R6531	T9606	T9602	parataxis	Biosciences,MLH1
R6532	T9607	T9606	dep	1,Biosciences
R6533	T9608	T9609	punct	:,50
R6534	T9609	T9607	prep	50,1
R6535	T9610	T9606	punct	;,Biosciences
R6536	T9611	T9606	compound	BD,Biosciences
R6537	T9612	T9606	punct	", ",Biosciences
R6538	T9613	T9606	npadvmod	http://www.bdbiosciences.com,Biosciences
R6539	T9614	T9606	punct	),Biosciences
R6540	T9615	T9602	punct	;,MLH1
R6541	T9616	T9617	compound	rabbit,anti-TopBP1
R6542	T9617	T9602	conj	anti-TopBP1,MLH1
R6543	T9618	T9617	punct	-,anti-TopBP1
R6544	T9619	T9620	punct	(,gift
R6545	T9620	T9617	parataxis	gift,anti-TopBP1
R6546	T9621	T9620	dep	1,gift
R6547	T9622	T9623	punct	:,100
R6548	T9623	T9621	prep	100,1
R6549	T9624	T9620	punct	;,gift
R6550	T9625	T9620	det	a,gift
R6551	T9626	T9620	prep	from,gift
R6552	T9627	T9628	compound	J.,Chen
R6553	T9628	T9626	pobj	Chen,from
R6554	T9629	T9620	punct	),gift
R6555	T9630	T9631	punct	[,22
R6556	T9631	T9617	parataxis	22,anti-TopBP1
R6557	T9632	T9631	punct	],22
R6558	T9633	T9617	punct	;,anti-TopBP1
R6559	T9634	T9635	nmod	mouse,H2A
R6560	T9635	T9617	conj	H2A,anti-TopBP1
R6561	T9636	T9635	punct	-,H2A
R6562	T9637	T9635	amod	anti-ubiquityl,H2A
R6563	T9638	T9635	punct	-,H2A
R6564	T9639	T9635	compound	histone,H2A
R6565	T9640	T9641	punct	(,Biotechnology
R6566	T9641	T9635	parataxis	Biotechnology,H2A
R6567	T9642	T9641	dep	1,Biotechnology
R6568	T9643	T9644	punct	:,200
R6569	T9644	T9642	prep	200,1
R6570	T9645	T9641	punct	;,Biotechnology
R6571	T9646	T9641	compound	Upstate,Biotechnology
R6572	T9647	T9641	punct	),Biotechnology
R6573	T9648	T9635	punct	;,H2A
R6574	T9649	T9650	compound	rabbit,anti-TRF2
R6575	T9650	T9635	conj	anti-TRF2,H2A
R6576	T9651	T9650	punct	-,anti-TRF2
R6577	T9652	T9653	punct	(,gift
R6578	T9653	T9650	parataxis	gift,anti-TRF2
R6579	T9654	T9653	dep	1,gift
R6580	T9655	T9656	punct	:,500
R6581	T9656	T9654	prep	500,1
R6582	T9657	T9653	punct	;,gift
R6583	T9658	T9653	det	a,gift
R6584	T9659	T9653	prep	from,gift
R6585	T9660	T9661	compound	T.,Lange
R6586	T9661	T9659	pobj	Lange,from
R6587	T9662	T9661	compound	de,Lange
R6588	T9663	T9653	punct	),gift
R6589	T9664	T9650	punct	;,anti-TRF2
R6590	T9665	T9650	cc	and,anti-TRF2
R6591	T9666	T9667	compound	rabbit,anti-BLM
R6592	T9667	T9650	conj	anti-BLM,anti-TRF2
R6593	T9668	T9667	punct	-,anti-BLM
R6594	T9669	T9670	punct	(,gift
R6595	T9670	T9667	parataxis	gift,anti-BLM
R6596	T9671	T9670	dep	1,gift
R6597	T9672	T9673	punct	:,50
R6598	T9673	T9671	prep	50,1
R6599	T9674	T9670	punct	;,gift
R6600	T9675	T9670	det	a,gift
R6601	T9676	T9670	prep	from,gift
R6602	T9677	T9678	compound	R.,Freire
R6603	T9678	T9676	pobj	Freire,from
R6604	T9679	T9670	punct	),gift
R6605	T9680	T9486	punct	.,were
R6606	T9682	T9683	det	All,antibodies
R6607	T9683	T9685	nsubjpass	antibodies,used
R6608	T9684	T9683	amod	secondary,antibodies
R6609	T9686	T9683	acl	conjugated,antibodies
R6610	T9687	T9686	prep	with,conjugated
R6611	T9688	T9689	preconj	either,488
R6612	T9689	T9687	pobj	488,with
R6613	T9690	T9689	nmod	Alexa,488
R6614	T9691	T9689	nmod	Fluor,488
R6615	T9692	T9689	cc	or,488
R6616	T9693	T9689	conj	594,488
R6617	T9694	T9695	punct	(,Probes
R6618	T9695	T9689	parataxis	Probes,488
R6619	T9696	T9695	compound	Molecular,Probes
R6620	T9697	T9695	punct	", ",Probes
R6621	T9698	T9695	npadvmod	http://probes.invitrogen.com/,Probes
R6622	T9699	T9695	punct	),Probes
R6623	T9700	T9685	auxpass	were,used
R6624	T9701	T9685	prep	at,used
R6625	T9702	T9703	det	a,dilution
R6626	T9703	T9701	pobj	dilution,at
R6627	T9704	T9703	prep	of,dilution
R6628	T9705	T9704	pobj	1,of
R6629	T9706	T9707	punct	:,"1,000"
R6630	T9707	T9705	prep	"1,000",1
R6631	T9708	T9685	punct	.,used
R6632	T9710	T9711	det	All,images
R6633	T9711	T9712	nsubjpass	images,taken
R6634	T9713	T9712	auxpass	were,taken
R6635	T9714	T9712	prep	with,taken
R6636	T9715	T9716	det	a,lens
R6637	T9716	T9714	pobj	lens,with
R6638	T9717	T9716	nummod	100,lens
R6639	T9718	T9717	punct	×,100
R6640	T9719	T9716	compound	objective,lens
R6641	T9720	T9712	prep	under,taken
R6642	T9721	T9722	compound	immersion,oil
R6643	T9722	T9720	pobj	oil,under
R6644	T9723	T9712	punct	.,taken
R6645	T9753	T9754	nmod	Metaphase,spermatocyte
R6646	T9754	T9756	compound	spermatocyte,spreads
R6647	T9755	T9753	nummod	I,Metaphase
R6648	T9757	T9756	cc	and,spreads
R6649	T9758	T9759	compound	OA,treatment
R6650	T9759	T9756	conj	treatment,spreads
R6651	T9760	T9759	punct	.,treatment
R6652	T9762	T9763	npadvmod	Metaphase,fixed
R6653	T9763	T9764	amod	fixed,spermatocytes
R6654	T9764	T9765	nsubj	spermatocytes,prepared
R6655	T9766	T9764	prep	from,spermatocytes
R6656	T9767	T9768	nummod	8,mo
R6657	T9768	T9770	npadvmod	mo,old
R6658	T9769	T9768	punct	-,mo
R6659	T9770	T9772	amod	old,homozygotes
R6660	T9771	T9770	punct	-,old
R6661	T9772	T9766	pobj	homozygotes,from
R6662	T9773	T9772	compound	Trip13RRB047,homozygotes
R6663	T9774	T9765	punct	", ",prepared
R6664	T9775	T9765	advcl	using,prepared
R6665	T9776	T9777	nummod	23,d
R6666	T9777	T9779	npadvmod	d,old
R6667	T9778	T9777	punct	-,d
R6668	T9779	T9781	amod	old,mice
R6669	T9780	T9779	punct	-,old
R6670	T9781	T9775	dobj	mice,using
R6671	T9782	T9783	amod	wild,type
R6672	T9783	T9781	compound	type,mice
R6673	T9784	T9783	punct	-,type
R6674	T9785	T9775	prep	as,using
R6675	T9786	T9785	pobj	control,as
R6676	T9787	T9765	punct	", ",prepared
R6677	T9788	T9765	aux	were,prepared
R6678	T9789	T9765	cc	and,prepared
R6679	T9790	T9765	conj	stained,prepared
R6680	T9791	T9790	prep	with,stained
R6681	T9792	T9791	pobj	Giemsa,with
R6682	T9793	T9794	mark	as,described
R6683	T9794	T9765	advcl	described,prepared
R6684	T9795	T9796	punct	[,64
R6685	T9796	T9765	parataxis	64,prepared
R6686	T9797	T9796	punct	],64
R6687	T9798	T9765	punct	.,prepared
R6688	T9800	T9801	prep	For,exposed
R6689	T9802	T9803	compound	OA,treatment
R6690	T9803	T9800	pobj	treatment,For
R6691	T9804	T9801	punct	", ",exposed
R6692	T9805	T9801	nsubjpass	cells,exposed
R6693	T9806	T9801	auxpass	were,exposed
R6694	T9807	T9801	prep	to,exposed
R6695	T9808	T9809	nummod	5,μM
R6696	T9809	T9810	compound	μM,OA
R6697	T9810	T9807	pobj	OA,to
R6698	T9811	T9812	punct	(,Calbiochem
R6699	T9812	T9810	parataxis	Calbiochem,OA
R6700	T9813	T9812	punct	", ",Calbiochem
R6701	T9814	T9812	npadvmod	http://www.emdbiosciences.com,Calbiochem
R6702	T9815	T9812	punct	),Calbiochem
R6703	T9816	T9801	prep	for,exposed
R6704	T9817	T9818	nummod	6,h
R6705	T9818	T9816	pobj	h,for
R6706	T9819	T9801	prep	at,exposed
R6707	T9820	T9821	nummod	32,°C
R6708	T9821	T9819	pobj	°C,at
R6709	T9822	T9801	prep	in,exposed
R6710	T9823	T9824	det	a,environment
R6711	T9824	T9822	pobj	environment,in
R6712	T9825	T9824	amod	humidified,environment
R6713	T9826	T9824	prep	of,environment
R6714	T9827	T9828	nummod	5,%
R6715	T9828	T9829	compound	%,CO2
R6716	T9829	T9826	pobj	CO2,of
R6717	T9830	T9801	prep	before,exposed
R6718	T9831	T9830	pcomp	spreading,before
R6719	T9832	T9833	punct	[,65
R6720	T9833	T9801	parataxis	65,exposed
R6721	T9834	T9833	punct	],65
R6722	T9835	T9801	punct	.,exposed
R6723	T9837	T9838	det	These,preparations
R6724	T9838	T9839	nsubjpass	preparations,stained
R6725	T9840	T9839	auxpass	were,stained
R6726	T9841	T9839	prep	with,stained
R6727	T9842	T9841	pobj	DAPI,with
R6728	T9843	T9844	aux	to,visualize
R6729	T9844	T9839	advcl	visualize,stained
R6730	T9845	T9846	compound	metaphase,nuclei
R6731	T9846	T9844	dobj	nuclei,visualize
R6732	T9847	T9846	cc	and,nuclei
R6733	T9848	T9846	conj	chromosomes,nuclei
R6734	T9849	T9839	punct	.,stained
R6735	T9864	T9865	amod	Phylogenetic,analyses
R6736	T9866	T9865	punct	.,analyses
R6737	T9868	T9869	compound	TRIP13,orthologs
R6738	T9869	T9870	nsubjpass	orthologs,identified
R6739	T9871	T9870	auxpass	were,identified
R6740	T9872	T9870	prep	by,identified
R6741	T9873	T9874	compound	BLASTP,searches
R6742	T9874	T9872	pobj	searches,by
R6743	T9875	T9874	prep	of,searches
R6744	T9876	T9875	pobj	Genbank,of
R6745	T9877	T9876	cc	and,Genbank
R6746	T9878	T9879	amod	other,sources
R6747	T9879	T9876	conj	sources,Genbank
R6748	T9880	T9879	acl	providing,sources
R6749	T9881	T9882	compound	gene,models
R6750	T9882	T9880	dobj	models,providing
R6751	T9883	T9884	amod	such,as
R6752	T9884	T9882	prep	as,models
R6753	T9885	T9884	pobj	Ensembl,as
R6754	T9886	T9870	punct	.,identified
R6755	T9888	T9889	det	The,orthologs
R6756	T9889	T9891	nsubjpass	orthologs,found
R6757	T9890	T9889	amod	selected,orthologs
R6758	T9892	T9891	aux	can,found
R6759	T9893	T9891	auxpass	be,found
R6760	T9894	T9891	prep	in,found
R6761	T9895	T9896	compound	Table,S1
R6762	T9896	T9894	pobj	S1,in
R6763	T9897	T9891	punct	.,found
R6764	T9899	T9900	compound	Amino,acid
R6765	T9900	T9901	compound	acid,alignments
R6766	T9901	T9902	nsubjpass	alignments,done
R6767	T9903	T9902	auxpass	were,done
R6768	T9904	T9902	prep	with,done
R6769	T9905	T9906	compound	Clustal,W
R6770	T9906	T9904	pobj	W,with
R6771	T9907	T9902	punct	", ",done
R6772	T9908	T9902	advcl	using,done
R6773	T9909	T9910	det	the,settings
R6774	T9910	T9908	dobj	settings,using
R6775	T9911	T9910	compound	default,settings
R6776	T9912	T9908	prep	with,using
R6777	T9913	T9912	cc	and,with
R6778	T9914	T9912	conj	without,with
R6779	T9915	T9914	pcomp	removing,without
R6780	T9916	T9917	det	the,regions
R6781	T9917	T9915	dobj	regions,removing
R6782	T9918	T9917	prep	outside,regions
R6783	T9919	T9918	prep	of,outside
R6784	T9920	T9921	det	the,domain
R6785	T9921	T9919	pobj	domain,of
R6786	T9922	T9923	nmod	AAA,ATPase
R6787	T9923	T9921	nmod	ATPase,domain
R6788	T9924	T9923	punct	-,ATPase
R6789	T9925	T9921	amod	central,domain
R6790	T9926	T9902	punct	.,done
R6791	T9928	T9929	det	The,trees
R6792	T9929	T9930	nsubjpass	trees,constructed
R6793	T9931	T9930	auxpass	were,constructed
R6794	T9932	T9930	prep	by,constructed
R6795	T9933	T9932	pcomp	using,by
R6796	T9934	T9935	det	the,method
R6797	T9935	T9933	dobj	method,using
R6798	T9936	T9937	npadvmod	neighbor,joining
R6799	T9937	T9935	amod	joining,method
R6800	T9938	T9937	punct	-,joining
R6801	T9939	T9935	prep	with,method
R6802	T9940	T9941	compound	Poisson,correction
R6803	T9941	T9939	pobj	correction,with
R6804	T9942	T9930	punct	.,constructed
R6805	T9944	T9945	det	The,reliability
R6806	T9945	T9946	nsubjpass	reliability,assessed
R6807	T9947	T9945	prep	of,reliability
R6808	T9948	T9949	amod	internal,branches
R6809	T9949	T9947	pobj	branches,of
R6810	T9950	T9946	auxpass	was,assessed
R6811	T9951	T9946	prep	by,assessed
R6812	T9952	T9951	pcomp	using,by
R6813	T9953	T9954	nummod	500,replicates
R6814	T9954	T9952	dobj	replicates,using
R6815	T9955	T9954	compound	bootstrap,replicates
R6816	T9956	T9946	punct	", ",assessed
R6817	T9957	T9946	cc	and,assessed
R6818	T9958	T9959	nsubjpass	sites,ignored
R6819	T9959	T9946	conj	ignored,assessed
R6820	T9960	T9958	prep	with,sites
R6821	T9961	T9960	pobj	gaps,with
R6822	T9962	T9959	auxpass	were,ignored
R6823	T9963	T9959	prep	in,ignored
R6824	T9964	T9965	det	this,analysis
R6825	T9965	T9963	pobj	analysis,in
R6826	T9966	T9959	punct	.,ignored
R6827	T9968	T9969	npadvmod	Neighbor,joining
R6828	T9969	T9971	amod	joining,searches
R6829	T9970	T9969	punct	-,joining
R6830	T9971	T9972	nsubjpass	searches,conducted
R6831	T9973	T9972	auxpass	were,conducted
R6832	T9974	T9972	prep	by,conducted
R6833	T9975	T9974	pcomp	using,by
R6834	T9976	T9977	det	the,program
R6835	T9977	T9975	dobj	program,using
R6836	T9978	T9977	compound	computer,program
R6837	T9979	T9977	appos	MEGA3,program
R6838	T9980	T9981	punct	[,66
R6839	T9981	T9972	parataxis	66,conducted
R6840	T9982	T9981	punct	],66
R6841	T9983	T9972	punct	.,conducted

PMC:1941754 / 39200-46401 JSONTXT 4 Projects

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PMC:1941754 / 39200-46401 JSON TXT 4 Projects