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PMC:1941754 / 11173-11955 JSONTXT 3 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1877 0-2 NN denotes RT
T1876 0-217 sentence denotes RT-PCR analysis of Trip13Gt expression (Figure 1D) revealed a low level of normally spliced transcripts in testes of homozygotes that is presumably a consequence of incomplete usage of the gene trap's splice acceptor.
T1879 2-3 HYPH denotes -
T1878 3-6 NN denotes PCR
T1880 7-15 NN denotes analysis
T1882 16-18 IN denotes of
T1883 19-27 NN denotes Trip13Gt
T1884 28-38 NN denotes expression
T1885 39-40 -LRB- denotes (
T1887 40-46 NN denotes Figure
T1886 47-49 NN denotes 1D
T1888 49-50 -RRB- denotes )
T1881 51-59 VBD denotes revealed
T1889 60-61 DT denotes a
T1891 62-65 JJ denotes low
T1890 66-71 NN denotes level
T1892 72-74 IN denotes of
T1893 75-83 RB denotes normally
T1894 84-91 VBN denotes spliced
T1895 92-103 NNS denotes transcripts
T1896 104-106 IN denotes in
T1897 107-113 NNS denotes testes
T1898 114-116 IN denotes of
T1899 117-128 NNS denotes homozygotes
T1900 129-133 WDT denotes that
T1901 134-136 VBZ denotes is
T1902 137-147 RB denotes presumably
T1903 148-149 DT denotes a
T1904 150-161 NN denotes consequence
T1905 162-164 IN denotes of
T1906 165-175 JJ denotes incomplete
T1907 176-181 NN denotes usage
T1908 182-184 IN denotes of
T1909 185-188 DT denotes the
T1911 189-193 NN denotes gene
T1910 194-198 NN denotes trap
T1913 198-200 POS denotes 's
T1914 201-207 NN denotes splice
T1912 208-216 NN denotes acceptor
T1915 216-217 . denotes .
T1916 217-445 sentence denotes Western blot analysis, using a polyclonal antibody raised against a peptide encoded by exon 3, revealed multiple species in wild-type and heterozygous testes, one of which corresponds to the expected size of 48 kDa (Figure 1E).
T1917 218-225 NNP denotes Western
T1918 226-230 NN denotes blot
T1919 231-239 NN denotes analysis
T1921 239-241 , denotes ,
T1922 241-246 VBG denotes using
T1923 247-248 DT denotes a
T1925 249-259 JJ denotes polyclonal
T1924 260-268 NN denotes antibody
T1926 269-275 VBN denotes raised
T1927 276-283 IN denotes against
T1928 284-285 DT denotes a
T1929 286-293 NN denotes peptide
T1930 294-301 VBN denotes encoded
T1931 302-304 IN denotes by
T1932 305-309 NN denotes exon
T1933 310-311 CD denotes 3
T1934 311-313 , denotes ,
T1920 313-321 VBD denotes revealed
T1935 322-330 JJ denotes multiple
T1936 331-338 NNS denotes species
T1937 339-341 IN denotes in
T1938 342-346 JJ denotes wild
T1940 346-347 HYPH denotes -
T1939 347-351 NN denotes type
T1942 352-355 CC denotes and
T1943 356-368 JJ denotes heterozygous
T1941 369-375 NNS denotes testes
T1944 375-377 , denotes ,
T1945 377-380 CD denotes one
T1947 381-383 IN denotes of
T1948 384-389 WDT denotes which
T1946 390-401 VBZ denotes corresponds
T1949 402-404 IN denotes to
T1950 405-408 DT denotes the
T1952 409-417 VBN denotes expected
T1951 418-422 NN denotes size
T1953 423-425 IN denotes of
T1954 426-428 CD denotes 48
T1955 429-432 NN denotes kDa
T1956 433-434 -LRB- denotes (
T1958 434-440 NN denotes Figure
T1957 441-443 NN denotes 1E
T1959 443-444 -RRB- denotes )
T1960 444-445 . denotes .
T1961 445-590 sentence denotes This and three other species were undetectable in homozygous mutant testes, but a reduced amount of an intense ∼38 kDa smaller band was present.
T1962 446-450 DT denotes This
T1964 451-454 CC denotes and
T1965 455-460 CD denotes three
T1967 461-466 JJ denotes other
T1966 467-474 NNS denotes species
T1963 475-479 VBD denotes were
T1968 480-492 JJ denotes undetectable
T1969 493-495 IN denotes in
T1970 496-506 JJ denotes homozygous
T1971 507-513 NN denotes mutant
T1972 514-520 NNS denotes testes
T1973 520-522 , denotes ,
T1974 522-525 CC denotes but
T1975 526-527 DT denotes a
T1977 528-535 VBN denotes reduced
T1976 536-542 NN denotes amount
T1979 543-545 IN denotes of
T1980 546-548 DT denotes an
T1982 549-556 JJ denotes intense
T1983 557-558 SYM denotes ∼
T1984 558-560 CD denotes 38
T1985 561-564 NN denotes kDa
T1986 565-572 JJR denotes smaller
T1981 573-577 NN denotes band
T1978 578-581 VBD denotes was
T1987 582-589 JJ denotes present
T1988 589-590 . denotes .
T1989 590-637 sentence denotes It is not clear if this corresponds to TRIP13.
T1990 591-593 PRP denotes It
T1991 594-596 VBZ denotes is
T1992 597-600 RB denotes not
T1993 601-606 JJ denotes clear
T1994 607-609 IN denotes if
T1996 610-614 DT denotes this
T1995 615-626 VBZ denotes corresponds
T1997 627-629 IN denotes to
T1998 630-636 NN denotes TRIP13
T1999 636-637 . denotes .
T2000 637-782 sentence denotes The greatly decreased Trip13 mRNA and predicted correct-length protein in mutants indicate that the Trip13RRB047 allele is severely hypomorphic.
T2001 638-641 DT denotes The
T2003 642-649 RB denotes greatly
T2004 650-659 VBN denotes decreased
T2005 660-666 NN denotes Trip13
T2002 667-671 NN denotes mRNA
T2007 672-675 CC denotes and
T2008 676-685 VBN denotes predicted
T2010 686-693 JJ denotes correct
T2012 693-694 HYPH denotes -
T2011 694-700 NN denotes length
T2009 701-708 NN denotes protein
T2013 709-711 IN denotes in
T2014 712-719 NNS denotes mutants
T2006 720-728 VBP denotes indicate
T2015 729-733 IN denotes that
T2017 734-737 DT denotes the
T2019 738-750 NN denotes Trip13RRB047
T2018 751-757 NN denotes allele
T2016 758-760 VBZ denotes is
T2020 761-769 RB denotes severely
T2021 770-781 JJ denotes hypomorphic
T2022 781-782 . denotes .
R1167 T1877 T1878 compound RT,PCR
R1168 T1878 T1880 compound PCR,analysis
R1169 T1879 T1878 punct -,PCR
R1170 T1880 T1881 nsubj analysis,revealed
R1171 T1882 T1880 prep of,analysis
R1172 T1883 T1884 compound Trip13Gt,expression
R1173 T1884 T1882 pobj expression,of
R1174 T1885 T1886 punct (,1D
R1175 T1886 T1880 parataxis 1D,analysis
R1176 T1887 T1886 compound Figure,1D
R1177 T1888 T1886 punct ),1D
R1178 T1889 T1890 det a,level
R1179 T1890 T1881 dobj level,revealed
R1180 T1891 T1890 amod low,level
R1181 T1892 T1890 prep of,level
R1182 T1893 T1894 advmod normally,spliced
R1183 T1894 T1895 amod spliced,transcripts
R1184 T1895 T1892 pobj transcripts,of
R1185 T1896 T1890 prep in,level
R1186 T1897 T1896 pobj testes,in
R1187 T1898 T1897 prep of,testes
R1188 T1899 T1898 pobj homozygotes,of
R1189 T1900 T1901 dep that,is
R1190 T1901 T1890 relcl is,level
R1191 T1902 T1901 advmod presumably,is
R1192 T1903 T1904 det a,consequence
R1193 T1904 T1901 attr consequence,is
R1194 T1905 T1904 prep of,consequence
R1195 T1906 T1907 amod incomplete,usage
R1196 T1907 T1905 pobj usage,of
R1197 T1908 T1907 prep of,usage
R1198 T1909 T1910 det the,trap
R1199 T1910 T1912 poss trap,acceptor
R1200 T1911 T1910 compound gene,trap
R1201 T1912 T1908 pobj acceptor,of
R1202 T1913 T1910 case 's,trap
R1203 T1914 T1912 compound splice,acceptor
R1204 T1915 T1881 punct .,revealed
R1205 T1917 T1918 compound Western,blot
R1206 T1918 T1919 compound blot,analysis
R1207 T1919 T1920 nsubj analysis,revealed
R1208 T1921 T1919 punct ", ",analysis
R1209 T1922 T1919 acl using,analysis
R1210 T1923 T1924 det a,antibody
R1211 T1924 T1922 dobj antibody,using
R1212 T1925 T1924 amod polyclonal,antibody
R1213 T1926 T1924 acl raised,antibody
R1214 T1927 T1926 prep against,raised
R1215 T1928 T1929 det a,peptide
R1216 T1929 T1927 pobj peptide,against
R1217 T1930 T1929 acl encoded,peptide
R1218 T1931 T1930 agent by,encoded
R1219 T1932 T1931 pobj exon,by
R1220 T1933 T1932 nummod 3,exon
R1221 T1934 T1920 punct ", ",revealed
R1222 T1935 T1936 amod multiple,species
R1223 T1936 T1920 dobj species,revealed
R1224 T1937 T1936 prep in,species
R1225 T1938 T1939 amod wild,type
R1226 T1939 T1941 nmod type,testes
R1227 T1940 T1939 punct -,type
R1228 T1941 T1937 pobj testes,in
R1229 T1942 T1939 cc and,type
R1230 T1943 T1939 conj heterozygous,type
R1231 T1944 T1936 punct ", ",species
R1232 T1945 T1946 dep one,corresponds
R1233 T1946 T1936 relcl corresponds,species
R1234 T1947 T1945 prep of,one
R1235 T1948 T1947 pobj which,of
R1236 T1949 T1946 prep to,corresponds
R1237 T1950 T1951 det the,size
R1238 T1951 T1949 pobj size,to
R1239 T1952 T1951 amod expected,size
R1240 T1953 T1951 prep of,size
R1241 T1954 T1955 nummod 48,kDa
R1242 T1955 T1953 pobj kDa,of
R1243 T1956 T1957 punct (,1E
R1244 T1957 T1920 parataxis 1E,revealed
R1245 T1958 T1957 compound Figure,1E
R1246 T1959 T1957 punct ),1E
R1247 T1960 T1920 punct .,revealed
R1248 T1962 T1963 nsubj This,were
R1249 T1964 T1962 cc and,This
R1250 T1965 T1966 nummod three,species
R1251 T1966 T1962 conj species,This
R1252 T1967 T1966 amod other,species
R1253 T1968 T1963 acomp undetectable,were
R1254 T1969 T1963 prep in,were
R1255 T1970 T1971 amod homozygous,mutant
R1256 T1971 T1972 compound mutant,testes
R1257 T1972 T1969 pobj testes,in
R1258 T1973 T1963 punct ", ",were
R1259 T1974 T1963 cc but,were
R1260 T1975 T1976 det a,amount
R1261 T1976 T1978 nsubj amount,was
R1262 T1977 T1976 amod reduced,amount
R1263 T1978 T1963 conj was,were
R1264 T1979 T1976 prep of,amount
R1265 T1980 T1981 det an,band
R1266 T1981 T1979 pobj band,of
R1267 T1982 T1981 amod intense,band
R1268 T1983 T1984 punct ∼,38
R1269 T1984 T1985 nummod 38,kDa
R1270 T1985 T1986 npadvmod kDa,smaller
R1271 T1986 T1981 amod smaller,band
R1272 T1987 T1978 acomp present,was
R1273 T1988 T1978 punct .,was
R1274 T1990 T1991 nsubj It,is
R1275 T1992 T1991 neg not,is
R1276 T1993 T1991 acomp clear,is
R1277 T1994 T1995 mark if,corresponds
R1278 T1995 T1991 ccomp corresponds,is
R1279 T1996 T1995 nsubj this,corresponds
R1280 T1997 T1995 prep to,corresponds
R1281 T1998 T1997 pobj TRIP13,to
R1282 T1999 T1991 punct .,is
R1283 T2001 T2002 det The,mRNA
R1284 T2002 T2006 nsubj mRNA,indicate
R1285 T2003 T2004 advmod greatly,decreased
R1286 T2004 T2002 amod decreased,mRNA
R1287 T2005 T2002 compound Trip13,mRNA
R1288 T2007 T2002 cc and,mRNA
R1289 T2008 T2009 amod predicted,protein
R1290 T2009 T2002 conj protein,mRNA
R1291 T2010 T2011 amod correct,length
R1292 T2011 T2009 compound length,protein
R1293 T2012 T2011 punct -,length
R1294 T2013 T2009 prep in,protein
R1295 T2014 T2013 pobj mutants,in
R1296 T2015 T2016 mark that,is
R1297 T2016 T2006 ccomp is,indicate
R1298 T2017 T2018 det the,allele
R1299 T2018 T2016 nsubj allele,is
R1300 T2019 T2018 compound Trip13RRB047,allele
R1301 T2020 T2021 advmod severely,hypomorphic
R1302 T2021 T2016 acomp hypomorphic,is
R1303 T2022 T2006 punct .,indicate