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PMC:1914394 / 57610-59166 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T30759 0-3 NN denotes RNA
T30760 4-14 NN denotes extraction
T30761 14-16 , denotes ,
T30762 16-23 JJ denotes reverse
T30763 24-37 NN denotes transcription
T30764 37-39 , denotes ,
T30765 39-42 CC denotes and
T30766 43-46 NN denotes PCR
T30767 46-47 . denotes .
T30768 47-279 sentence denotes Total RNA was isolated from dissected peripheral retina using TRIzol reagent (Invitrogen, http://www.invitrogen.com) followed by digestion with RNase-free DNase (DNA-free, Ambion, http://www.ambion.com) to remove DNA contamination.
T30769 48-53 JJ denotes Total
T30770 54-57 NN denotes RNA
T30772 58-61 VBD denotes was
T30771 62-70 VBN denotes isolated
T30773 71-75 IN denotes from
T30774 76-85 VBN denotes dissected
T30776 86-96 JJ denotes peripheral
T30775 97-103 NN denotes retina
T30777 104-109 VBG denotes using
T30778 110-116 NN denotes TRIzol
T30779 117-124 NN denotes reagent
T30780 125-126 -LRB- denotes (
T30781 126-136 NNP denotes Invitrogen
T30782 136-138 , denotes ,
T30783 138-163 NN denotes http://www.invitrogen.com
T30784 163-164 -RRB- denotes )
T30785 165-173 VBN denotes followed
T30786 174-176 IN denotes by
T30787 177-186 NN denotes digestion
T30788 187-191 IN denotes with
T30789 192-197 NN denotes RNase
T30791 197-198 HYPH denotes -
T30790 198-202 JJ denotes free
T30792 203-208 NN denotes DNase
T30793 209-210 -LRB- denotes (
T30794 210-213 NN denotes DNA
T30795 213-214 HYPH denotes -
T30796 214-218 JJ denotes free
T30797 218-220 , denotes ,
T30798 220-226 NNP denotes Ambion
T30799 226-228 , denotes ,
T30800 228-249 NN denotes http://www.ambion.com
T30801 249-250 -RRB- denotes )
T30802 251-253 TO denotes to
T30803 254-260 VB denotes remove
T30804 261-264 NN denotes DNA
T30805 265-278 NN denotes contamination
T30806 278-279 . denotes .
T30807 279-411 sentence denotes First-strand cDNA was synthesized from 0.2–0.5 μg of total RNA using the SuperScript II first-strand synthesis system (Invitrogen).
T30808 280-285 JJ denotes First
T30810 285-286 HYPH denotes -
T30809 286-292 NN denotes strand
T30811 293-297 NN denotes cDNA
T30813 298-301 VBD denotes was
T30812 302-313 VBN denotes synthesized
T30814 314-318 IN denotes from
T30815 319-322 CD denotes 0.2
T30817 322-323 SYM denotes –
T30816 323-326 CD denotes 0.5
T30818 327-329 NN denotes μg
T30819 330-332 IN denotes of
T30820 333-338 JJ denotes total
T30821 339-342 NN denotes RNA
T30822 343-348 VBG denotes using
T30823 349-352 DT denotes the
T30825 353-364 NNP denotes SuperScript
T30826 365-367 CD denotes II
T30827 368-373 JJ denotes first
T30829 373-374 HYPH denotes -
T30828 374-380 NN denotes strand
T30830 381-390 NN denotes synthesis
T30824 391-397 NN denotes system
T30831 398-399 -LRB- denotes (
T30832 399-409 NNP denotes Invitrogen
T30833 409-410 -RRB- denotes )
T30834 410-411 . denotes .
T30835 411-447 sentence denotes PCR primers are listed in Table S2.
T30836 412-415 NN denotes PCR
T30837 416-423 NNS denotes primers
T30839 424-427 VBP denotes are
T30838 428-434 VBN denotes listed
T30840 435-437 IN denotes in
T30841 438-443 NN denotes Table
T30842 444-446 NN denotes S2
T30843 446-447 . denotes .
T30844 447-561 sentence denotes Real-time quantitative PCR was performed using an Applied Biosystems (http://appliedbiosystems.com) PRISM 7900HT.
T30845 448-452 JJ denotes Real
T30847 452-453 HYPH denotes -
T30846 453-457 NN denotes time
T30849 458-470 JJ denotes quantitative
T30848 471-474 NN denotes PCR
T30851 475-478 VBD denotes was
T30850 479-488 VBN denotes performed
T30852 489-494 VBG denotes using
T30853 495-497 DT denotes an
T30855 498-505 NNP denotes Applied
T30856 506-516 NNP denotes Biosystems
T30857 517-518 -LRB- denotes (
T30858 518-546 NN denotes http://appliedbiosystems.com
T30859 546-547 -RRB- denotes )
T30860 548-553 NNP denotes PRISM
T30854 554-560 NN denotes 7900HT
T30861 560-561 . denotes .
T30862 561-719 sentence denotes Tests were run in duplicate on three separate biological samples with SYBR Green PCR Master Mix (Applied Biosystems) exactly as we described previously [71].
T30863 562-567 NNS denotes Tests
T30865 568-572 VBD denotes were
T30864 573-576 VBN denotes run
T30866 577-579 IN denotes in
T30867 580-589 NN denotes duplicate
T30868 590-592 IN denotes on
T30869 593-598 CD denotes three
T30871 599-607 JJ denotes separate
T30872 608-618 JJ denotes biological
T30870 619-626 NNS denotes samples
T30873 627-631 IN denotes with
T30874 632-636 NN denotes SYBR
T30876 637-642 JJ denotes Green
T30877 643-646 NN denotes PCR
T30878 647-653 NNP denotes Master
T30875 654-657 NNP denotes Mix
T30879 658-659 -LRB- denotes (
T30881 659-666 NNP denotes Applied
T30880 667-677 NNP denotes Biosystems
T30882 677-678 -RRB- denotes )
T30883 679-686 RB denotes exactly
T30885 687-689 IN denotes as
T30886 690-692 PRP denotes we
T30884 693-702 VBD denotes described
T30887 703-713 RB denotes previously
T30888 714-715 -LRB- denotes [
T30889 715-717 CD denotes 71
T30890 717-718 -RRB- denotes ]
T30891 718-719 . denotes .
T30892 719-1052 sentence denotes Briefly, master stocks were prepared such that each 10-μl reaction contained 5 μl of SYBR Green PCR Master Mix, 0.1 μl of each forward and reverse primer (stock 50 μM), 0.8 μl of blue H2O (0.73% Blue Food Colour; McCormick, http://www.mccormick.com), 2 μl of diluted cDNA template, and 2 μl of yellow H2O (0.73% Yellow Food Colour).
T30893 720-727 RB denotes Briefly
T30895 727-729 , denotes ,
T30896 729-735 NN denotes master
T30897 736-742 NNS denotes stocks
T30898 743-747 VBD denotes were
T30894 748-756 VBN denotes prepared
T30899 757-761 JJ denotes such
T30901 762-766 IN denotes that
T30902 767-771 DT denotes each
T30904 772-774 CD denotes 10
T30906 774-775 HYPH denotes -
T30905 775-777 NN denotes μl
T30903 778-786 NN denotes reaction
T30900 787-796 VBD denotes contained
T30907 797-798 CD denotes 5
T30908 799-801 NN denotes μl
T30909 802-804 IN denotes of
T30910 805-809 NNP denotes SYBR
T30912 810-815 JJ denotes Green
T30913 816-819 NN denotes PCR
T30914 820-826 NN denotes Master
T30911 827-830 NN denotes Mix
T30915 830-832 , denotes ,
T30916 832-835 CD denotes 0.1
T30917 836-838 NN denotes μl
T30918 839-841 IN denotes of
T30919 842-846 DT denotes each
T30921 847-854 JJ denotes forward
T30922 855-858 CC denotes and
T30923 859-866 JJ denotes reverse
T30920 867-873 NN denotes primer
T30924 874-875 -LRB- denotes (
T30926 875-880 NN denotes stock
T30927 881-883 CD denotes 50
T30925 884-886 NN denotes μM
T30928 886-887 -RRB- denotes )
T30929 887-889 , denotes ,
T30930 889-892 CD denotes 0.8
T30931 893-895 NN denotes μl
T30932 896-898 IN denotes of
T30933 899-903 JJ denotes blue
T30934 904-907 NN denotes H2O
T30935 908-909 -LRB- denotes (
T30937 909-913 CD denotes 0.73
T30938 913-914 NN denotes %
T30940 915-919 NNP denotes Blue
T30941 920-924 NNP denotes Food
T30939 925-931 NNP denotes Colour
T30942 931-932 : denotes ;
T30936 933-942 NNP denotes McCormick
T30943 942-944 , denotes ,
T30944 944-968 NN denotes http://www.mccormick.com
T30945 968-969 -RRB- denotes )
T30946 969-971 , denotes ,
T30947 971-972 CD denotes 2
T30948 973-975 NN denotes μl
T30949 976-978 IN denotes of
T30950 979-986 VBN denotes diluted
T30952 987-991 NN denotes cDNA
T30951 992-1000 NN denotes template
T30953 1000-1002 , denotes ,
T30954 1002-1005 CC denotes and
T30955 1006-1007 CD denotes 2
T30956 1008-1010 NN denotes μl
T30957 1011-1013 IN denotes of
T30958 1014-1020 JJ denotes yellow
T30959 1021-1024 NN denotes H2O
T30960 1025-1026 -LRB- denotes (
T30962 1026-1030 CD denotes 0.73
T30963 1030-1031 NN denotes %
T30964 1032-1038 JJ denotes Yellow
T30965 1039-1043 NN denotes Food
T30961 1044-1050 NN denotes Colour
T30966 1050-1051 -RRB- denotes )
T30967 1051-1052 . denotes .
T30968 1052-1160 sentence denotes PCR consisted of 40 cycles of denaturation at 95 °C for 15 s and annealing and extension at 55 °C for 30 s.
T30969 1053-1056 NN denotes PCR
T30970 1057-1066 VBD denotes consisted
T30971 1067-1069 IN denotes of
T30972 1070-1072 CD denotes 40
T30973 1073-1079 NNS denotes cycles
T30974 1080-1082 IN denotes of
T30975 1083-1095 NN denotes denaturation
T30976 1096-1098 IN denotes at
T30977 1099-1101 CD denotes 95
T30978 1102-1104 NN denotes °C
T30979 1105-1108 IN denotes for
T30980 1109-1111 CD denotes 15
T30981 1112-1113 NN denotes s
T30982 1114-1117 CC denotes and
T30983 1118-1127 NN denotes annealing
T30984 1128-1131 CC denotes and
T30985 1132-1141 NN denotes extension
T30986 1142-1144 IN denotes at
T30987 1145-1147 CD denotes 55
T30988 1148-1150 NN denotes °C
T30989 1151-1154 IN denotes for
T30990 1155-1157 CD denotes 30
T30991 1158-1159 NN denotes s
T30992 1159-1160 . denotes .
T30993 1160-1261 sentence denotes An additional cycle (95 °C, 15 s, 60 °C) generated a dissociation curve to confirm a single product.
T30994 1161-1163 DT denotes An
T30996 1164-1174 JJ denotes additional
T30995 1175-1180 NN denotes cycle
T30998 1181-1182 -LRB- denotes (
T31000 1182-1184 CD denotes 95
T31001 1185-1187 NN denotes °C
T31002 1187-1189 , denotes ,
T31003 1189-1191 CD denotes 15
T31004 1192-1193 NN denotes s
T31005 1193-1195 , denotes ,
T31006 1195-1197 CD denotes 60
T30999 1198-1200 NN denotes °C
T31007 1200-1201 -RRB- denotes )
T30997 1202-1211 VBD denotes generated
T31008 1212-1213 DT denotes a
T31010 1214-1226 NN denotes dissociation
T31009 1227-1232 NN denotes curve
T31011 1233-1235 TO denotes to
T31012 1236-1243 VB denotes confirm
T31013 1244-1245 DT denotes a
T31015 1246-1252 JJ denotes single
T31014 1253-1260 NN denotes product
T31016 1260-1261 . denotes .
T31017 1261-1488 sentence denotes The cycle quantity required to reach a threshold in the linear range was determined and compared to a standard curve for each primer set generated by five 3-fold dilutions of genomic DNA or cDNA samples of known concentration.
T31018 1262-1265 DT denotes The
T31020 1266-1271 NN denotes cycle
T31019 1272-1280 NN denotes quantity
T31022 1281-1289 VBN denotes required
T31023 1290-1292 TO denotes to
T31024 1293-1298 VB denotes reach
T31025 1299-1300 DT denotes a
T31026 1301-1310 NN denotes threshold
T31027 1311-1313 IN denotes in
T31028 1314-1317 DT denotes the
T31030 1318-1324 JJ denotes linear
T31029 1325-1330 NN denotes range
T31031 1331-1334 VBD denotes was
T31021 1335-1345 VBN denotes determined
T31032 1346-1349 CC denotes and
T31033 1350-1358 VBN denotes compared
T31034 1359-1361 IN denotes to
T31035 1362-1363 DT denotes a
T31037 1364-1372 JJ denotes standard
T31036 1373-1378 NN denotes curve
T31038 1379-1382 IN denotes for
T31039 1383-1387 DT denotes each
T31041 1388-1394 NN denotes primer
T31040 1395-1398 NN denotes set
T31042 1399-1408 VBN denotes generated
T31043 1409-1411 IN denotes by
T31044 1412-1416 CD denotes five
T31046 1417-1418 CD denotes 3
T31047 1418-1419 HYPH denotes -
T31048 1419-1423 JJ denotes fold
T31045 1424-1433 NNS denotes dilutions
T31049 1434-1436 IN denotes of
T31050 1437-1444 JJ denotes genomic
T31052 1445-1448 NN denotes DNA
T31053 1449-1451 CC denotes or
T31054 1452-1456 NN denotes cDNA
T31051 1457-1464 NNS denotes samples
T31055 1465-1467 IN denotes of
T31056 1468-1473 JJ denotes known
T31057 1474-1487 NN denotes concentration
T31058 1487-1488 . denotes .
T31059 1488-1556 sentence denotes Values obtained for test RNAs were normalized to Hprt1 mRNA levels.
T31060 1489-1495 NNS denotes Values
T31061 1496-1504 VBN denotes obtained
T31062 1505-1508 IN denotes for
T31064 1509-1513 NN denotes test
T31065 1514-1518 NNS denotes RNAs
T31066 1519-1523 VBD denotes were
T31063 1524-1534 VBN denotes normalized
T31067 1535-1537 IN denotes to
T31068 1538-1543 NN denotes Hprt1
T31069 1544-1548 NN denotes mRNA
T31070 1549-1555 NNS denotes levels
T31071 1555-1556 . denotes .
R9134 T30759 T30760 compound RNA,extraction
R9135 T30761 T30760 punct ", ",extraction
R9136 T30762 T30763 amod reverse,transcription
R9137 T30763 T30760 conj transcription,extraction
R9138 T30764 T30763 punct ", ",transcription
R9139 T30765 T30763 cc and,transcription
R9140 T30766 T30763 conj PCR,transcription
R9141 T30767 T30766 punct .,PCR
R9142 T30769 T30770 amod Total,RNA
R9143 T30770 T30771 nsubjpass RNA,isolated
R9144 T30772 T30771 auxpass was,isolated
R9145 T30773 T30771 prep from,isolated
R9146 T30774 T30775 amod dissected,retina
R9147 T30775 T30773 pobj retina,from
R9148 T30776 T30775 amod peripheral,retina
R9149 T30777 T30771 advcl using,isolated
R9150 T30778 T30779 compound TRIzol,reagent
R9151 T30779 T30777 dobj reagent,using
R9152 T30780 T30781 punct (,Invitrogen
R9153 T30781 T30779 parataxis Invitrogen,reagent
R9154 T30782 T30781 punct ", ",Invitrogen
R9155 T30783 T30781 npadvmod http://www.invitrogen.com,Invitrogen
R9156 T30784 T30781 punct ),Invitrogen
R9157 T30785 T30779 acl followed,reagent
R9158 T30786 T30785 agent by,followed
R9159 T30787 T30786 pobj digestion,by
R9160 T30788 T30787 prep with,digestion
R9161 T30789 T30790 npadvmod RNase,free
R9162 T30790 T30792 amod free,DNase
R9163 T30791 T30790 punct -,free
R9164 T30792 T30788 pobj DNase,with
R9165 T30793 T30792 punct (,DNase
R9166 T30794 T30792 appos DNA,DNase
R9167 T30795 T30794 punct -,DNA
R9168 T30796 T30794 amod free,DNA
R9169 T30797 T30798 punct ", ",Ambion
R9170 T30798 T30792 parataxis Ambion,DNase
R9171 T30799 T30798 punct ", ",Ambion
R9172 T30800 T30798 npadvmod http://www.ambion.com,Ambion
R9173 T30801 T30798 punct ),Ambion
R9174 T30802 T30803 aux to,remove
R9175 T30803 T30785 advcl remove,followed
R9176 T30804 T30805 compound DNA,contamination
R9177 T30805 T30803 dobj contamination,remove
R9178 T30806 T30771 punct .,isolated
R9179 T30808 T30809 amod First,strand
R9180 T30809 T30811 compound strand,cDNA
R9181 T30810 T30809 punct -,strand
R9182 T30811 T30812 nsubjpass cDNA,synthesized
R9183 T30813 T30812 auxpass was,synthesized
R9184 T30814 T30812 prep from,synthesized
R9185 T30815 T30816 quantmod 0.2,0.5
R9186 T30816 T30818 nummod 0.5,μg
R9187 T30817 T30816 punct –,0.5
R9188 T30818 T30814 pobj μg,from
R9189 T30819 T30818 prep of,μg
R9190 T30820 T30821 amod total,RNA
R9191 T30821 T30819 pobj RNA,of
R9192 T30822 T30812 advcl using,synthesized
R9193 T30823 T30824 det the,system
R9194 T30824 T30822 dobj system,using
R9195 T30825 T30824 nmod SuperScript,system
R9196 T30826 T30825 nummod II,SuperScript
R9197 T30827 T30828 amod first,strand
R9198 T30828 T30830 compound strand,synthesis
R9199 T30829 T30828 punct -,strand
R9200 T30830 T30824 compound synthesis,system
R9201 T30831 T30832 punct (,Invitrogen
R9202 T30832 T30822 parataxis Invitrogen,using
R9203 T30833 T30832 punct ),Invitrogen
R9204 T30834 T30812 punct .,synthesized
R9205 T30836 T30837 compound PCR,primers
R9206 T30837 T30838 nsubjpass primers,listed
R9207 T30839 T30838 auxpass are,listed
R9208 T30840 T30838 prep in,listed
R9209 T30841 T30842 compound Table,S2
R9210 T30842 T30840 pobj S2,in
R9211 T30843 T30838 punct .,listed
R9212 T30845 T30846 amod Real,time
R9213 T30846 T30848 nmod time,PCR
R9214 T30847 T30846 punct -,time
R9215 T30848 T30850 nsubjpass PCR,performed
R9216 T30849 T30848 amod quantitative,PCR
R9217 T30851 T30850 auxpass was,performed
R9218 T30852 T30850 advcl using,performed
R9219 T30853 T30854 det an,7900HT
R9220 T30854 T30852 dobj 7900HT,using
R9221 T30855 T30856 nmod Applied,Biosystems
R9222 T30856 T30854 nmod Biosystems,7900HT
R9223 T30857 T30856 punct (,Biosystems
R9224 T30858 T30856 appos http://appliedbiosystems.com,Biosystems
R9225 T30859 T30854 punct ),7900HT
R9226 T30860 T30854 compound PRISM,7900HT
R9227 T30861 T30850 punct .,performed
R9228 T30863 T30864 nsubjpass Tests,run
R9229 T30865 T30864 auxpass were,run
R9230 T30866 T30864 prep in,run
R9231 T30867 T30866 pobj duplicate,in
R9232 T30868 T30864 prep on,run
R9233 T30869 T30870 nummod three,samples
R9234 T30870 T30868 pobj samples,on
R9235 T30871 T30870 amod separate,samples
R9236 T30872 T30870 amod biological,samples
R9237 T30873 T30864 prep with,run
R9238 T30874 T30875 nmod SYBR,Mix
R9239 T30875 T30873 pobj Mix,with
R9240 T30876 T30875 amod Green,Mix
R9241 T30877 T30875 compound PCR,Mix
R9242 T30878 T30875 compound Master,Mix
R9243 T30879 T30880 punct (,Biosystems
R9244 T30880 T30875 parataxis Biosystems,Mix
R9245 T30881 T30880 compound Applied,Biosystems
R9246 T30882 T30880 punct ),Biosystems
R9247 T30883 T30884 advmod exactly,described
R9248 T30884 T30864 advcl described,run
R9249 T30885 T30884 mark as,described
R9250 T30886 T30884 nsubj we,described
R9251 T30887 T30884 advmod previously,described
R9252 T30888 T30889 punct [,71
R9253 T30889 T30884 parataxis 71,described
R9254 T30890 T30889 punct ],71
R9255 T30891 T30864 punct .,run
R9256 T30893 T30894 advmod Briefly,prepared
R9257 T30895 T30894 punct ", ",prepared
R9258 T30896 T30897 compound master,stocks
R9259 T30897 T30894 nsubjpass stocks,prepared
R9260 T30898 T30894 auxpass were,prepared
R9261 T30899 T30900 amod such,contained
R9262 T30900 T30894 advcl contained,prepared
R9263 T30901 T30900 mark that,contained
R9264 T30902 T30903 det each,reaction
R9265 T30903 T30900 nsubj reaction,contained
R9266 T30904 T30905 nummod 10,μl
R9267 T30905 T30903 compound μl,reaction
R9268 T30906 T30905 punct -,μl
R9269 T30907 T30908 nummod 5,μl
R9270 T30908 T30900 dobj μl,contained
R9271 T30909 T30908 prep of,μl
R9272 T30910 T30911 nmod SYBR,Mix
R9273 T30911 T30909 pobj Mix,of
R9274 T30912 T30911 amod Green,Mix
R9275 T30913 T30911 compound PCR,Mix
R9276 T30914 T30911 compound Master,Mix
R9277 T30915 T30908 punct ", ",μl
R9278 T30916 T30917 nummod 0.1,μl
R9279 T30917 T30908 conj μl,μl
R9280 T30918 T30917 prep of,μl
R9281 T30919 T30920 det each,primer
R9282 T30920 T30918 pobj primer,of
R9283 T30921 T30920 nmod forward,primer
R9284 T30922 T30921 cc and,forward
R9285 T30923 T30921 conj reverse,forward
R9286 T30924 T30925 punct (,μM
R9287 T30925 T30920 parataxis μM,primer
R9288 T30926 T30925 nmod stock,μM
R9289 T30927 T30925 nummod 50,μM
R9290 T30928 T30925 punct ),μM
R9291 T30929 T30917 punct ", ",μl
R9292 T30930 T30931 nummod 0.8,μl
R9293 T30931 T30917 conj μl,μl
R9294 T30932 T30931 prep of,μl
R9295 T30933 T30934 amod blue,H2O
R9296 T30934 T30932 pobj H2O,of
R9297 T30935 T30936 punct (,McCormick
R9298 T30936 T30934 parataxis McCormick,H2O
R9299 T30937 T30938 nummod 0.73,%
R9300 T30938 T30939 compound %,Colour
R9301 T30939 T30936 dep Colour,McCormick
R9302 T30940 T30939 compound Blue,Colour
R9303 T30941 T30939 compound Food,Colour
R9304 T30942 T30936 punct ;,McCormick
R9305 T30943 T30936 punct ", ",McCormick
R9306 T30944 T30936 npadvmod http://www.mccormick.com,McCormick
R9307 T30945 T30936 punct ),McCormick
R9308 T30946 T30931 punct ", ",μl
R9309 T30947 T30948 nummod 2,μl
R9310 T30948 T30931 conj μl,μl
R9311 T30949 T30948 prep of,μl
R9312 T30950 T30951 amod diluted,template
R9313 T30951 T30949 pobj template,of
R9314 T30952 T30951 compound cDNA,template
R9315 T30953 T30948 punct ", ",μl
R9316 T30954 T30948 cc and,μl
R9317 T30955 T30956 nummod 2,μl
R9318 T30956 T30948 conj μl,μl
R9319 T30957 T30956 prep of,μl
R9320 T30958 T30959 amod yellow,H2O
R9321 T30959 T30957 pobj H2O,of
R9322 T30960 T30961 punct (,Colour
R9323 T30961 T30959 parataxis Colour,H2O
R9324 T30962 T30963 nummod 0.73,%
R9325 T30963 T30961 nmod %,Colour
R9326 T30964 T30961 amod Yellow,Colour
R9327 T30965 T30961 compound Food,Colour
R9328 T30966 T30961 punct ),Colour
R9329 T30967 T30894 punct .,prepared
R9330 T30969 T30970 nsubj PCR,consisted
R9331 T30971 T30970 prep of,consisted
R9332 T30972 T30973 nummod 40,cycles
R9333 T30973 T30971 pobj cycles,of
R9334 T30974 T30973 prep of,cycles
R9335 T30975 T30974 pobj denaturation,of
R9336 T30976 T30973 prep at,cycles
R9337 T30977 T30978 nummod 95,°C
R9338 T30978 T30976 pobj °C,at
R9339 T30979 T30973 prep for,cycles
R9340 T30980 T30981 nummod 15,s
R9341 T30981 T30979 pobj s,for
R9342 T30982 T30973 cc and,cycles
R9343 T30983 T30973 conj annealing,cycles
R9344 T30984 T30983 cc and,annealing
R9345 T30985 T30983 conj extension,annealing
R9346 T30986 T30983 prep at,annealing
R9347 T30987 T30988 nummod 55,°C
R9348 T30988 T30986 pobj °C,at
R9349 T30989 T30983 prep for,annealing
R9350 T30990 T30991 nummod 30,s
R9351 T30991 T30989 pobj s,for
R9352 T30992 T30970 punct .,consisted
R9353 T30994 T30995 det An,cycle
R9354 T30995 T30997 nsubj cycle,generated
R9355 T30996 T30995 amod additional,cycle
R9356 T30998 T30999 punct (,°C
R9357 T30999 T30995 parataxis °C,cycle
R9358 T31000 T31001 nummod 95,°C
R9359 T31001 T30999 dep °C,°C
R9360 T31002 T30999 punct ", ",°C
R9361 T31003 T31004 nummod 15,s
R9362 T31004 T30999 dep s,°C
R9363 T31005 T30999 punct ", ",°C
R9364 T31006 T30999 nummod 60,°C
R9365 T31007 T30999 punct ),°C
R9366 T31008 T31009 det a,curve
R9367 T31009 T30997 dobj curve,generated
R9368 T31010 T31009 compound dissociation,curve
R9369 T31011 T31012 aux to,confirm
R9370 T31012 T30997 advcl confirm,generated
R9371 T31013 T31014 det a,product
R9372 T31014 T31012 dobj product,confirm
R9373 T31015 T31014 amod single,product
R9374 T31016 T30997 punct .,generated
R9375 T31018 T31019 det The,quantity
R9376 T31019 T31021 nsubjpass quantity,determined
R9377 T31020 T31019 compound cycle,quantity
R9378 T31022 T31019 acl required,quantity
R9379 T31023 T31024 aux to,reach
R9380 T31024 T31022 xcomp reach,required
R9381 T31025 T31026 det a,threshold
R9382 T31026 T31024 dobj threshold,reach
R9383 T31027 T31024 prep in,reach
R9384 T31028 T31029 det the,range
R9385 T31029 T31027 pobj range,in
R9386 T31030 T31029 amod linear,range
R9387 T31031 T31021 auxpass was,determined
R9388 T31032 T31021 cc and,determined
R9389 T31033 T31021 conj compared,determined
R9390 T31034 T31033 prep to,compared
R9391 T31035 T31036 det a,curve
R9392 T31036 T31034 pobj curve,to
R9393 T31037 T31036 amod standard,curve
R9394 T31038 T31036 prep for,curve
R9395 T31039 T31040 det each,set
R9396 T31040 T31038 pobj set,for
R9397 T31041 T31040 compound primer,set
R9398 T31042 T31040 acl generated,set
R9399 T31043 T31042 agent by,generated
R9400 T31044 T31045 nummod five,dilutions
R9401 T31045 T31043 pobj dilutions,by
R9402 T31046 T31045 nummod 3,dilutions
R9403 T31047 T31046 punct -,3
R9404 T31048 T31046 amod fold,3
R9405 T31049 T31045 prep of,dilutions
R9406 T31050 T31051 amod genomic,samples
R9407 T31051 T31049 pobj samples,of
R9408 T31052 T31051 nmod DNA,samples
R9409 T31053 T31052 cc or,DNA
R9410 T31054 T31052 conj cDNA,DNA
R9411 T31055 T31051 prep of,samples
R9412 T31056 T31057 amod known,concentration
R9413 T31057 T31055 pobj concentration,of
R9414 T31058 T31021 punct .,determined
R9415 T31060 T31061 nsubj Values,obtained
R9416 T31062 T31063 mark for,normalized
R9417 T31063 T31061 advcl normalized,obtained
R9418 T31064 T31065 compound test,RNAs
R9419 T31065 T31063 nsubjpass RNAs,normalized
R9420 T31066 T31063 auxpass were,normalized
R9421 T31067 T31063 prep to,normalized
R9422 T31068 T31069 compound Hprt1,mRNA
R9423 T31069 T31070 compound mRNA,levels
R9424 T31070 T31067 pobj levels,to
R9425 T31071 T31061 punct .,obtained