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PMC:1914394 / 36644-38867 JSONTXT 3 Projects

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Id Subject Object Predicate Lexical cue
T20222 0-2 IN denotes As
T20223 3-8 VBN denotes noted
T20225 9-14 RB denotes above
T20226 14-16 , denotes ,
T20227 16-20 NN denotes E2f3
T20229 21-24 CC denotes and
T20230 25-27 NN denotes Rb
T20228 28-36 NN denotes staining
T20231 37-39 IN denotes in
T20232 40-44 NNS denotes SACs
T20224 45-48 VBD denotes was
T20233 49-53 CC denotes both
T20234 54-61 JJ denotes nuclear
T20235 62-65 CC denotes and
T20236 66-77 JJ denotes cytoplasmic
T20237 78-79 -LRB- denotes (
T20239 79-85 NN denotes Figure
T20238 86-88 NN denotes 5A
T20240 89-92 CC denotes and
T20241 93-95 NN denotes 5B
T20242 95-96 -RRB- denotes )
T20243 96-97 . denotes .
T20244 97-216 sentence denotes The antibody that worked in immunostaining recognizes a C-terminal region and thus, does not distinguish a/b isoforms.
T20245 98-101 DT denotes The
T20246 102-110 NN denotes antibody
T20248 111-115 WDT denotes that
T20249 116-122 VBD denotes worked
T20250 123-125 IN denotes in
T20251 126-140 NN denotes immunostaining
T20247 141-151 VBZ denotes recognizes
T20252 152-153 DT denotes a
T20254 154-155 NN denotes C
T20256 155-156 HYPH denotes -
T20255 156-164 JJ denotes terminal
T20253 165-171 NN denotes region
T20257 172-175 CC denotes and
T20258 176-180 RB denotes thus
T20260 180-182 , denotes ,
T20261 182-186 VBZ denotes does
T20262 187-190 RB denotes not
T20259 191-202 VB denotes distinguish
T20263 203-204 NN denotes a
T20265 204-205 HYPH denotes /
T20264 205-206 NN denotes b
T20266 207-215 NNS denotes isoforms
T20267 215-216 . denotes .
T20268 216-318 sentence denotes To our knowledge, the subcellular location of E2f3 isoforms has not been determined in any cell type.
T20269 217-219 IN denotes To
T20271 220-223 PRP$ denotes our
T20272 224-233 NN denotes knowledge
T20273 233-235 , denotes ,
T20274 235-238 DT denotes the
T20276 239-250 JJ denotes subcellular
T20275 251-259 NN denotes location
T20277 260-262 IN denotes of
T20278 263-267 NN denotes E2f3
T20279 268-276 NNS denotes isoforms
T20280 277-280 VBZ denotes has
T20281 281-284 RB denotes not
T20282 285-289 VBN denotes been
T20270 290-300 VBN denotes determined
T20283 301-303 IN denotes in
T20284 304-307 DT denotes any
T20286 308-312 NN denotes cell
T20285 313-317 NN denotes type
T20287 317-318 . denotes .
T20288 318-512 sentence denotes To verify the dual locations of E2f3 and to determine which isoforms were present in retina, we analyzed nuclear and cytoplasmic fractions by Western blot at different times during development.
T20289 319-321 TO denotes To
T20290 322-328 VB denotes verify
T20292 329-332 DT denotes the
T20294 333-337 JJ denotes dual
T20293 338-347 NNS denotes locations
T20295 348-350 IN denotes of
T20296 351-355 NN denotes E2f3
T20297 356-359 CC denotes and
T20298 360-362 TO denotes to
T20299 363-372 VB denotes determine
T20300 373-378 WDT denotes which
T20301 379-387 NNS denotes isoforms
T20302 388-392 VBD denotes were
T20303 393-400 JJ denotes present
T20304 401-403 IN denotes in
T20305 404-410 NN denotes retina
T20306 410-412 , denotes ,
T20307 412-414 PRP denotes we
T20291 415-423 VBD denotes analyzed
T20308 424-431 JJ denotes nuclear
T20310 432-435 CC denotes and
T20311 436-447 JJ denotes cytoplasmic
T20309 448-457 NNS denotes fractions
T20312 458-460 IN denotes by
T20313 461-468 NNP denotes Western
T20314 469-473 NN denotes blot
T20315 474-476 IN denotes at
T20316 477-486 JJ denotes different
T20317 487-492 NNS denotes times
T20318 493-499 IN denotes during
T20319 500-511 NN denotes development
T20320 511-512 . denotes .
T20321 512-656 sentence denotes Analysis with the pan-E2f3 antibody (sc-878, Santa Cruz Biotechnology) detected a 55-kD E2f3a species and a 40-kD E2f3b polypeptide (Figure 6).
T20322 513-521 NN denotes Analysis
T20324 522-526 IN denotes with
T20325 527-530 DT denotes the
T20327 531-539 JJ denotes pan-E2f3
T20326 540-548 NN denotes antibody
T20328 549-550 -LRB- denotes (
T20329 550-552 NN denotes sc
T20330 552-553 HYPH denotes -
T20331 553-556 CD denotes 878
T20332 556-558 , denotes ,
T20334 558-563 NNP denotes Santa
T20335 564-568 NNP denotes Cruz
T20333 569-582 NNP denotes Biotechnology
T20336 582-583 -RRB- denotes )
T20323 584-592 VBD denotes detected
T20337 593-594 DT denotes a
T20339 595-597 CD denotes 55
T20341 597-598 HYPH denotes -
T20340 598-600 NN denotes kD
T20342 601-606 NN denotes E2f3a
T20338 607-614 NNS denotes species
T20343 615-618 CC denotes and
T20344 619-620 DT denotes a
T20346 621-623 CD denotes 40
T20348 623-624 HYPH denotes -
T20347 624-626 NN denotes kD
T20349 627-632 NN denotes E2f3b
T20345 633-644 NN denotes polypeptide
T20350 645-646 -LRB- denotes (
T20351 646-652 NN denotes Figure
T20352 653-654 CD denotes 6
T20353 654-655 -RRB- denotes )
T20354 655-656 . denotes .
T20355 656-843 sentence denotes To confirm that the upper species in our retinal lysates was E2f3a, we exploited novel mice that lack E2f3 exon 1a and thus express E2f3b exclusively (R. O. and G. L., unpublished data).
T20356 657-659 TO denotes To
T20357 660-667 VB denotes confirm
T20359 668-672 IN denotes that
T20361 673-676 DT denotes the
T20363 677-682 JJ denotes upper
T20362 683-690 NNS denotes species
T20364 691-693 IN denotes in
T20365 694-697 PRP$ denotes our
T20367 698-705 JJ denotes retinal
T20366 706-713 NNS denotes lysates
T20360 714-717 VBD denotes was
T20368 718-723 NN denotes E2f3a
T20369 723-725 , denotes ,
T20370 725-727 PRP denotes we
T20358 728-737 VBD denotes exploited
T20371 738-743 JJ denotes novel
T20372 744-748 NNS denotes mice
T20373 749-753 WDT denotes that
T20374 754-758 VBP denotes lack
T20375 759-763 NN denotes E2f3
T20377 764-768 NN denotes exon
T20376 769-771 NN denotes 1a
T20378 772-775 CC denotes and
T20379 776-780 RB denotes thus
T20380 781-788 VBP denotes express
T20381 789-794 NN denotes E2f3b
T20382 795-806 RB denotes exclusively
T20383 807-808 -LRB- denotes (
T20384 808-810 NNP denotes R.
T20385 811-813 NNP denotes O.
T20386 814-817 CC denotes and
T20387 818-820 NNP denotes G.
T20388 821-823 NNP denotes L.
T20389 823-825 , denotes ,
T20390 825-836 JJ denotes unpublished
T20391 837-841 NNS denotes data
T20392 841-842 -RRB- denotes )
T20393 842-843 . denotes .
T20394 843-926 sentence denotes The genotyping strategy is discussed in detail later and is outlined in Figure 7A.
T20395 844-847 DT denotes The
T20397 848-858 NN denotes genotyping
T20396 859-867 NN denotes strategy
T20399 868-870 VBZ denotes is
T20398 871-880 VBN denotes discussed
T20400 881-883 IN denotes in
T20401 884-890 NN denotes detail
T20402 891-896 RB denotes later
T20403 897-900 CC denotes and
T20404 901-903 VBZ denotes is
T20405 904-912 VBN denotes outlined
T20406 913-915 IN denotes in
T20407 916-922 NN denotes Figure
T20408 923-925 NN denotes 7A
T20409 925-926 . denotes .
T20410 926-1021 sentence denotes Western analysis confirmed that the upper band was absent in E2f3a−/− mice (Figures 6 and S8).
T20411 927-934 NNP denotes Western
T20412 935-943 NN denotes analysis
T20413 944-953 VBD denotes confirmed
T20414 954-958 IN denotes that
T20416 959-962 DT denotes the
T20418 963-968 JJ denotes upper
T20417 969-973 NN denotes band
T20415 974-977 VBD denotes was
T20419 978-984 JJ denotes absent
T20420 985-987 IN denotes in
T20421 988-993 NN denotes E2f3a
T20423 993-994 SYM denotes −
T20424 994-995 HYPH denotes /
T20425 995-996 SYM denotes −
T20422 997-1001 NNS denotes mice
T20426 1002-1003 -LRB- denotes (
T20428 1003-1010 NNS denotes Figures
T20429 1011-1012 CD denotes 6
T20430 1013-1016 CC denotes and
T20427 1017-1019 NN denotes S8
T20431 1019-1020 -RRB- denotes )
T20432 1020-1021 . denotes .
T20433 1021-1182 sentence denotes Consistent with the drop in E2f3-expressing cells during WT retinal maturation (Figure 5A), the total amount of E2f3a was less at P18 compared to P0 (Figure 6).
T20434 1022-1032 JJ denotes Consistent
T20436 1033-1037 IN denotes with
T20437 1038-1041 DT denotes the
T20438 1042-1046 NN denotes drop
T20439 1047-1049 IN denotes in
T20440 1050-1054 NN denotes E2f3
T20442 1054-1055 HYPH denotes -
T20441 1055-1065 VBG denotes expressing
T20443 1066-1071 NNS denotes cells
T20444 1072-1078 IN denotes during
T20445 1079-1081 NN denotes WT
T20447 1082-1089 JJ denotes retinal
T20446 1090-1100 NN denotes maturation
T20448 1101-1102 -LRB- denotes (
T20450 1102-1108 NN denotes Figure
T20449 1109-1111 NN denotes 5A
T20451 1111-1112 -RRB- denotes )
T20452 1112-1114 , denotes ,
T20453 1114-1117 DT denotes the
T20455 1118-1123 JJ denotes total
T20454 1124-1130 NN denotes amount
T20456 1131-1133 IN denotes of
T20457 1134-1139 NN denotes E2f3a
T20435 1140-1143 VBD denotes was
T20458 1144-1148 JJR denotes less
T20459 1149-1151 IN denotes at
T20460 1152-1155 NN denotes P18
T20461 1156-1164 VBN denotes compared
T20462 1165-1167 IN denotes to
T20463 1168-1170 NN denotes P0
T20464 1171-1172 -LRB- denotes (
T20465 1172-1178 NN denotes Figure
T20466 1179-1180 CD denotes 6
T20467 1180-1181 -RRB- denotes )
T20468 1181-1182 . denotes .
T20469 1182-1240 sentence denotes E2f3b was present in similar amounts at both time points.
T20470 1183-1188 NN denotes E2f3b
T20471 1189-1192 VBD denotes was
T20472 1193-1200 JJ denotes present
T20473 1201-1203 IN denotes in
T20474 1204-1211 JJ denotes similar
T20475 1212-1219 NNS denotes amounts
T20476 1220-1222 IN denotes at
T20477 1223-1227 DT denotes both
T20479 1228-1232 NN denotes time
T20478 1233-1239 NNS denotes points
T20480 1239-1240 . denotes .
T20481 1240-1396 sentence denotes At P0 and P18, E2f3a was present in both nuclear and cytoplasmic fractions, but in marked contrast, E2f3b was exclusively nuclear at both times (Figure 6).
T20482 1241-1243 IN denotes At
T20484 1244-1246 NN denotes P0
T20485 1247-1250 CC denotes and
T20486 1251-1254 NN denotes P18
T20487 1254-1256 , denotes ,
T20488 1256-1261 NN denotes E2f3a
T20483 1262-1265 VBD denotes was
T20489 1266-1273 JJ denotes present
T20490 1274-1276 IN denotes in
T20491 1277-1281 CC denotes both
T20492 1282-1289 JJ denotes nuclear
T20494 1290-1293 CC denotes and
T20495 1294-1305 JJ denotes cytoplasmic
T20493 1306-1315 NNS denotes fractions
T20496 1315-1317 , denotes ,
T20497 1317-1320 CC denotes but
T20498 1321-1323 IN denotes in
T20500 1324-1330 JJ denotes marked
T20501 1331-1339 NN denotes contrast
T20502 1339-1341 , denotes ,
T20503 1341-1346 NN denotes E2f3b
T20499 1347-1350 VBD denotes was
T20504 1351-1362 RB denotes exclusively
T20505 1363-1370 JJ denotes nuclear
T20506 1371-1373 IN denotes at
T20507 1374-1378 DT denotes both
T20508 1379-1384 NNS denotes times
T20509 1385-1386 -LRB- denotes (
T20510 1386-1392 NN denotes Figure
T20511 1393-1394 CD denotes 6
T20512 1394-1395 -RRB- denotes )
T20513 1395-1396 . denotes .
T20514 1396-1597 sentence denotes Two closely migrating E2f3a bands were detected, more clearly evident at P18, of which the faster migrating species was dominant in nuclear and the slower species was dominant in cytoplasm (Figure 6).
T20515 1397-1400 CD denotes Two
T20517 1401-1408 RB denotes closely
T20518 1409-1418 VBG denotes migrating
T20519 1419-1424 NN denotes E2f3a
T20516 1425-1430 NNS denotes bands
T20521 1431-1435 VBD denotes were
T20520 1436-1444 VBN denotes detected
T20522 1444-1446 , denotes ,
T20523 1446-1450 RBR denotes more
T20524 1451-1458 RB denotes clearly
T20525 1459-1466 JJ denotes evident
T20526 1467-1469 IN denotes at
T20527 1470-1473 NN denotes P18
T20528 1473-1475 , denotes ,
T20529 1475-1477 IN denotes of
T20531 1478-1483 WDT denotes which
T20532 1484-1487 DT denotes the
T20534 1488-1494 RBR denotes faster
T20535 1495-1504 VBG denotes migrating
T20533 1505-1512 NN denotes species
T20530 1513-1516 VBD denotes was
T20536 1517-1525 JJ denotes dominant
T20537 1526-1528 IN denotes in
T20538 1529-1536 JJ denotes nuclear
T20539 1537-1540 CC denotes and
T20540 1541-1544 DT denotes the
T20542 1545-1551 JJR denotes slower
T20541 1552-1559 NN denotes species
T20543 1560-1563 VBD denotes was
T20544 1564-1572 JJ denotes dominant
T20545 1573-1575 IN denotes in
T20546 1576-1585 NN denotes cytoplasm
T20547 1586-1587 -LRB- denotes (
T20548 1587-1593 NN denotes Figure
T20549 1594-1595 CD denotes 6
T20550 1595-1596 -RRB- denotes )
T20551 1596-1597 . denotes .
T20552 1597-1706 sentence denotes The identity of both as E2f3a species was confirmed by their absence in the P18 E2f3a KO retina (Figure S8).
T20553 1598-1601 DT denotes The
T20554 1602-1610 NN denotes identity
T20556 1611-1613 IN denotes of
T20557 1614-1618 DT denotes both
T20558 1619-1621 IN denotes as
T20559 1622-1627 NN denotes E2f3a
T20560 1628-1635 NNS denotes species
T20561 1636-1639 VBD denotes was
T20555 1640-1649 VBN denotes confirmed
T20562 1650-1652 IN denotes by
T20563 1653-1658 PRP$ denotes their
T20564 1659-1666 NN denotes absence
T20565 1667-1669 IN denotes in
T20566 1670-1673 DT denotes the
T20568 1674-1677 NN denotes P18
T20569 1678-1683 NN denotes E2f3a
T20570 1684-1686 NN denotes KO
T20567 1687-1693 NN denotes retina
T20571 1694-1695 -LRB- denotes (
T20573 1695-1701 NN denotes Figure
T20572 1702-1704 NN denotes S8
T20574 1704-1705 -RRB- denotes )
T20575 1705-1706 . denotes .
T20576 1706-1974 sentence denotes Analysis of Pou4f2, a nuclear transcription factor expressed in ganglion cells, showed that nuclear proteins had not contaminated the cytoplasmic fraction, and analysis of Slc18a3, a cytoplasmic SAC marker, confirmed that the reverse had also not occurred (Figure 6).
T20577 1707-1715 NN denotes Analysis
T20579 1716-1718 IN denotes of
T20580 1719-1725 NN denotes Pou4f2
T20581 1725-1727 , denotes ,
T20582 1727-1728 DT denotes a
T20584 1729-1736 JJ denotes nuclear
T20585 1737-1750 NN denotes transcription
T20583 1751-1757 NN denotes factor
T20586 1758-1767 VBN denotes expressed
T20587 1768-1770 IN denotes in
T20588 1771-1779 NN denotes ganglion
T20589 1780-1785 NNS denotes cells
T20590 1785-1787 , denotes ,
T20578 1787-1793 VBD denotes showed
T20591 1794-1798 IN denotes that
T20593 1799-1806 JJ denotes nuclear
T20594 1807-1815 NN denotes proteins
T20595 1816-1819 VBD denotes had
T20596 1820-1823 RB denotes not
T20592 1824-1836 VBN denotes contaminated
T20597 1837-1840 DT denotes the
T20599 1841-1852 JJ denotes cytoplasmic
T20598 1853-1861 NN denotes fraction
T20600 1861-1863 , denotes ,
T20601 1863-1866 CC denotes and
T20602 1867-1875 NN denotes analysis
T20604 1876-1878 IN denotes of
T20605 1879-1886 NN denotes Slc18a3
T20606 1886-1888 , denotes ,
T20607 1888-1889 DT denotes a
T20609 1890-1901 JJ denotes cytoplasmic
T20610 1902-1905 NN denotes SAC
T20608 1906-1912 NN denotes marker
T20611 1912-1914 , denotes ,
T20603 1914-1923 VBD denotes confirmed
T20612 1924-1928 IN denotes that
T20614 1929-1932 DT denotes the
T20615 1933-1940 NN denotes reverse
T20616 1941-1944 VBD denotes had
T20617 1945-1949 RB denotes also
T20618 1950-1953 RB denotes not
T20613 1954-1962 VBN denotes occurred
T20619 1963-1964 -LRB- denotes (
T20620 1964-1970 NN denotes Figure
T20621 1971-1972 CD denotes 6
T20622 1972-1973 -RRB- denotes )
T20623 1973-1974 . denotes .
T20624 1974-2223 sentence denotes These data show, to our knowledge for the first time, that E2f3a and E2f3b exhibit distinct patterns of subcellular distribution, and raise the possibility that E2f3a localization may be regulated by as yet unknown post-translational modifications.
T20625 1975-1980 DT denotes These
T20626 1981-1985 NNS denotes data
T20627 1986-1990 VBP denotes show
T20628 1990-1992 , denotes ,
T20629 1992-1994 IN denotes to
T20631 1995-1998 PRP$ denotes our
T20632 1999-2008 NN denotes knowledge
T20630 2009-2012 IN denotes for
T20633 2013-2016 DT denotes the
T20635 2017-2022 JJ denotes first
T20634 2023-2027 NN denotes time
T20636 2027-2029 , denotes ,
T20637 2029-2033 IN denotes that
T20639 2034-2039 NN denotes E2f3a
T20640 2040-2043 CC denotes and
T20641 2044-2049 NN denotes E2f3b
T20638 2050-2057 VBP denotes exhibit
T20642 2058-2066 JJ denotes distinct
T20643 2067-2075 NNS denotes patterns
T20644 2076-2078 IN denotes of
T20645 2079-2090 JJ denotes subcellular
T20646 2091-2103 NN denotes distribution
T20647 2103-2105 , denotes ,
T20648 2105-2108 CC denotes and
T20649 2109-2114 VB denotes raise
T20650 2115-2118 DT denotes the
T20651 2119-2130 NN denotes possibility
T20652 2131-2135 IN denotes that
T20654 2136-2141 NN denotes E2f3a
T20655 2142-2154 NN denotes localization
T20656 2155-2158 MD denotes may
T20657 2159-2161 VB denotes be
T20653 2162-2171 VBN denotes regulated
T20658 2172-2174 IN denotes by
T20659 2175-2177 RB denotes as
T20660 2178-2181 RB denotes yet
T20661 2182-2189 JJ denotes unknown
T20663 2190-2208 JJ denotes post-translational
T20662 2209-2222 NNS denotes modifications
T20664 2222-2223 . denotes .
R5725 T20222 T20223 mark As,noted
R5726 T20223 T20224 advcl noted,was
R5727 T20225 T20223 advmod above,noted
R5728 T20226 T20224 punct ", ",was
R5729 T20227 T20228 nmod E2f3,staining
R5730 T20228 T20224 nsubj staining,was
R5731 T20229 T20227 cc and,E2f3
R5732 T20230 T20227 conj Rb,E2f3
R5733 T20231 T20228 prep in,staining
R5734 T20232 T20231 pobj SACs,in
R5735 T20233 T20234 preconj both,nuclear
R5736 T20234 T20224 acomp nuclear,was
R5737 T20235 T20234 cc and,nuclear
R5738 T20236 T20234 conj cytoplasmic,nuclear
R5739 T20237 T20238 punct (,5A
R5740 T20238 T20224 parataxis 5A,was
R5741 T20239 T20238 compound Figure,5A
R5742 T20240 T20238 cc and,5A
R5743 T20241 T20238 conj 5B,5A
R5744 T20242 T20238 punct ),5A
R5745 T20243 T20224 punct .,was
R5746 T20245 T20246 det The,antibody
R5747 T20246 T20247 nsubj antibody,recognizes
R5748 T20248 T20249 dep that,worked
R5749 T20249 T20246 relcl worked,antibody
R5750 T20250 T20249 prep in,worked
R5751 T20251 T20250 pobj immunostaining,in
R5752 T20252 T20253 det a,region
R5753 T20253 T20247 dobj region,recognizes
R5754 T20254 T20255 npadvmod C,terminal
R5755 T20255 T20253 amod terminal,region
R5756 T20256 T20255 punct -,terminal
R5757 T20257 T20247 cc and,recognizes
R5758 T20258 T20259 advmod thus,distinguish
R5759 T20259 T20247 conj distinguish,recognizes
R5760 T20260 T20259 punct ", ",distinguish
R5761 T20261 T20259 aux does,distinguish
R5762 T20262 T20259 neg not,distinguish
R5763 T20263 T20264 compound a,b
R5764 T20264 T20266 compound b,isoforms
R5765 T20265 T20264 punct /,b
R5766 T20266 T20259 dobj isoforms,distinguish
R5767 T20267 T20247 punct .,recognizes
R5768 T20269 T20270 prep To,determined
R5769 T20271 T20272 poss our,knowledge
R5770 T20272 T20269 pobj knowledge,To
R5771 T20273 T20270 punct ", ",determined
R5772 T20274 T20275 det the,location
R5773 T20275 T20270 nsubjpass location,determined
R5774 T20276 T20275 amod subcellular,location
R5775 T20277 T20275 prep of,location
R5776 T20278 T20279 compound E2f3,isoforms
R5777 T20279 T20277 pobj isoforms,of
R5778 T20280 T20270 aux has,determined
R5779 T20281 T20270 neg not,determined
R5780 T20282 T20270 auxpass been,determined
R5781 T20283 T20270 prep in,determined
R5782 T20284 T20285 det any,type
R5783 T20285 T20283 pobj type,in
R5784 T20286 T20285 compound cell,type
R5785 T20287 T20270 punct .,determined
R5786 T20289 T20290 aux To,verify
R5787 T20290 T20291 advcl verify,analyzed
R5788 T20292 T20293 det the,locations
R5789 T20293 T20290 dobj locations,verify
R5790 T20294 T20293 amod dual,locations
R5791 T20295 T20293 prep of,locations
R5792 T20296 T20295 pobj E2f3,of
R5793 T20297 T20290 cc and,verify
R5794 T20298 T20299 aux to,determine
R5795 T20299 T20290 conj determine,verify
R5796 T20300 T20301 det which,isoforms
R5797 T20301 T20302 dep isoforms,were
R5798 T20302 T20299 ccomp were,determine
R5799 T20303 T20302 acomp present,were
R5800 T20304 T20302 prep in,were
R5801 T20305 T20304 pobj retina,in
R5802 T20306 T20291 punct ", ",analyzed
R5803 T20307 T20291 nsubj we,analyzed
R5804 T20308 T20309 amod nuclear,fractions
R5805 T20309 T20291 dobj fractions,analyzed
R5806 T20310 T20308 cc and,nuclear
R5807 T20311 T20308 conj cytoplasmic,nuclear
R5808 T20312 T20291 prep by,analyzed
R5809 T20313 T20314 compound Western,blot
R5810 T20314 T20312 pobj blot,by
R5811 T20315 T20291 prep at,analyzed
R5812 T20316 T20317 amod different,times
R5813 T20317 T20315 pobj times,at
R5814 T20318 T20317 prep during,times
R5815 T20319 T20318 pobj development,during
R5816 T20320 T20291 punct .,analyzed
R5817 T20322 T20323 nsubj Analysis,detected
R5818 T20324 T20322 prep with,Analysis
R5819 T20325 T20326 det the,antibody
R5820 T20326 T20324 pobj antibody,with
R5821 T20327 T20326 amod pan-E2f3,antibody
R5822 T20328 T20326 punct (,antibody
R5823 T20329 T20326 appos sc,antibody
R5824 T20330 T20329 punct -,sc
R5825 T20331 T20329 nummod 878,sc
R5826 T20332 T20333 punct ", ",Biotechnology
R5827 T20333 T20326 parataxis Biotechnology,antibody
R5828 T20334 T20335 compound Santa,Cruz
R5829 T20335 T20333 compound Cruz,Biotechnology
R5830 T20336 T20333 punct ),Biotechnology
R5831 T20337 T20338 det a,species
R5832 T20338 T20323 dobj species,detected
R5833 T20339 T20340 nummod 55,kD
R5834 T20340 T20338 compound kD,species
R5835 T20341 T20340 punct -,kD
R5836 T20342 T20338 compound E2f3a,species
R5837 T20343 T20338 cc and,species
R5838 T20344 T20345 det a,polypeptide
R5839 T20345 T20338 conj polypeptide,species
R5840 T20346 T20347 nummod 40,kD
R5841 T20347 T20345 compound kD,polypeptide
R5842 T20348 T20347 punct -,kD
R5843 T20349 T20345 compound E2f3b,polypeptide
R5844 T20350 T20351 punct (,Figure
R5845 T20351 T20323 parataxis Figure,detected
R5846 T20352 T20351 nummod 6,Figure
R5847 T20353 T20351 punct ),Figure
R5848 T20354 T20323 punct .,detected
R5849 T20356 T20357 aux To,confirm
R5850 T20357 T20358 advcl confirm,exploited
R5851 T20359 T20360 mark that,was
R5852 T20360 T20357 ccomp was,confirm
R5853 T20361 T20362 det the,species
R5854 T20362 T20360 nsubj species,was
R5855 T20363 T20362 amod upper,species
R5856 T20364 T20362 prep in,species
R5857 T20365 T20366 poss our,lysates
R5858 T20366 T20364 pobj lysates,in
R5859 T20367 T20366 amod retinal,lysates
R5860 T20368 T20360 attr E2f3a,was
R5861 T20369 T20358 punct ", ",exploited
R5862 T20370 T20358 nsubj we,exploited
R5863 T20371 T20372 amod novel,mice
R5864 T20372 T20358 dobj mice,exploited
R5865 T20373 T20374 dep that,lack
R5866 T20374 T20372 relcl lack,mice
R5867 T20375 T20376 compound E2f3,1a
R5868 T20376 T20374 dobj 1a,lack
R5869 T20377 T20376 compound exon,1a
R5870 T20378 T20374 cc and,lack
R5871 T20379 T20380 advmod thus,express
R5872 T20380 T20374 conj express,lack
R5873 T20381 T20380 dobj E2f3b,express
R5874 T20382 T20380 advmod exclusively,express
R5875 T20383 T20384 punct (,R.
R5876 T20384 T20380 meta R.,express
R5877 T20385 T20384 nmod O.,R.
R5878 T20386 T20384 cc and,R.
R5879 T20387 T20384 conj G.,R.
R5880 T20388 T20387 nmod L.,G.
R5881 T20389 T20387 punct ", ",G.
R5882 T20390 T20391 amod unpublished,data
R5883 T20391 T20387 conj data,G.
R5884 T20392 T20391 punct ),data
R5885 T20393 T20358 punct .,exploited
R5886 T20395 T20396 det The,strategy
R5887 T20396 T20398 nsubjpass strategy,discussed
R5888 T20397 T20396 compound genotyping,strategy
R5889 T20399 T20398 auxpass is,discussed
R5890 T20400 T20398 prep in,discussed
R5891 T20401 T20400 pobj detail,in
R5892 T20402 T20398 advmod later,discussed
R5893 T20403 T20398 cc and,discussed
R5894 T20404 T20405 auxpass is,outlined
R5895 T20405 T20398 conj outlined,discussed
R5896 T20406 T20405 prep in,outlined
R5897 T20407 T20408 compound Figure,7A
R5898 T20408 T20406 pobj 7A,in
R5899 T20409 T20398 punct .,discussed
R5900 T20411 T20412 compound Western,analysis
R5901 T20412 T20413 nsubj analysis,confirmed
R5902 T20414 T20415 mark that,was
R5903 T20415 T20413 ccomp was,confirmed
R5904 T20416 T20417 det the,band
R5905 T20417 T20415 nsubj band,was
R5906 T20418 T20417 amod upper,band
R5907 T20419 T20415 acomp absent,was
R5908 T20420 T20415 prep in,was
R5909 T20421 T20422 nmod E2f3a,mice
R5910 T20422 T20420 pobj mice,in
R5911 T20423 T20421 punct −,E2f3a
R5912 T20424 T20421 punct /,E2f3a
R5913 T20425 T20421 punct −,E2f3a
R5914 T20426 T20427 punct (,S8
R5915 T20427 T20413 parataxis S8,confirmed
R5916 T20428 T20427 nmod Figures,S8
R5917 T20429 T20427 nummod 6,S8
R5918 T20430 T20427 cc and,S8
R5919 T20431 T20427 punct ),S8
R5920 T20432 T20413 punct .,confirmed
R5921 T20434 T20435 advcl Consistent,was
R5922 T20436 T20434 prep with,Consistent
R5923 T20437 T20438 det the,drop
R5924 T20438 T20436 pobj drop,with
R5925 T20439 T20438 prep in,drop
R5926 T20440 T20441 npadvmod E2f3,expressing
R5927 T20441 T20443 amod expressing,cells
R5928 T20442 T20441 punct -,expressing
R5929 T20443 T20439 pobj cells,in
R5930 T20444 T20438 prep during,drop
R5931 T20445 T20446 nmod WT,maturation
R5932 T20446 T20444 pobj maturation,during
R5933 T20447 T20446 amod retinal,maturation
R5934 T20448 T20449 punct (,5A
R5935 T20449 T20438 parataxis 5A,drop
R5936 T20450 T20449 compound Figure,5A
R5937 T20451 T20449 punct ),5A
R5938 T20452 T20435 punct ", ",was
R5939 T20453 T20454 det the,amount
R5940 T20454 T20435 nsubj amount,was
R5941 T20455 T20454 amod total,amount
R5942 T20456 T20454 prep of,amount
R5943 T20457 T20456 pobj E2f3a,of
R5944 T20458 T20435 acomp less,was
R5945 T20459 T20435 prep at,was
R5946 T20460 T20459 pobj P18,at
R5947 T20461 T20435 prep compared,was
R5948 T20462 T20461 prep to,compared
R5949 T20463 T20462 pobj P0,to
R5950 T20464 T20465 punct (,Figure
R5951 T20465 T20435 parataxis Figure,was
R5952 T20466 T20465 nummod 6,Figure
R5953 T20467 T20465 punct ),Figure
R5954 T20468 T20435 punct .,was
R5955 T20470 T20471 nsubj E2f3b,was
R5956 T20472 T20471 acomp present,was
R5957 T20473 T20471 prep in,was
R5958 T20474 T20475 amod similar,amounts
R5959 T20475 T20473 pobj amounts,in
R5960 T20476 T20471 prep at,was
R5961 T20477 T20478 det both,points
R5962 T20478 T20476 pobj points,at
R5963 T20479 T20478 compound time,points
R5964 T20480 T20471 punct .,was
R5965 T20482 T20483 prep At,was
R5966 T20484 T20482 pobj P0,At
R5967 T20485 T20484 cc and,P0
R5968 T20486 T20484 conj P18,P0
R5969 T20487 T20483 punct ", ",was
R5970 T20488 T20483 nsubj E2f3a,was
R5971 T20489 T20483 acomp present,was
R5972 T20490 T20483 prep in,was
R5973 T20491 T20492 preconj both,nuclear
R5974 T20492 T20493 amod nuclear,fractions
R5975 T20493 T20490 pobj fractions,in
R5976 T20494 T20492 cc and,nuclear
R5977 T20495 T20492 conj cytoplasmic,nuclear
R5978 T20496 T20483 punct ", ",was
R5979 T20497 T20483 cc but,was
R5980 T20498 T20499 prep in,was
R5981 T20499 T20483 conj was,was
R5982 T20500 T20501 amod marked,contrast
R5983 T20501 T20498 pobj contrast,in
R5984 T20502 T20499 punct ", ",was
R5985 T20503 T20499 nsubj E2f3b,was
R5986 T20504 T20505 advmod exclusively,nuclear
R5987 T20505 T20499 acomp nuclear,was
R5988 T20506 T20499 prep at,was
R5989 T20507 T20508 det both,times
R5990 T20508 T20506 pobj times,at
R5991 T20509 T20510 punct (,Figure
R5992 T20510 T20499 parataxis Figure,was
R5993 T20511 T20510 nummod 6,Figure
R5994 T20512 T20510 punct ),Figure
R5995 T20513 T20499 punct .,was
R5996 T20515 T20516 nummod Two,bands
R5997 T20516 T20520 nsubjpass bands,detected
R5998 T20517 T20518 advmod closely,migrating
R5999 T20518 T20516 amod migrating,bands
R6000 T20519 T20516 compound E2f3a,bands
R6001 T20521 T20520 auxpass were,detected
R6002 T20522 T20520 punct ", ",detected
R6003 T20523 T20524 advmod more,clearly
R6004 T20524 T20525 advmod clearly,evident
R6005 T20525 T20520 advcl evident,detected
R6006 T20526 T20525 prep at,evident
R6007 T20527 T20526 pobj P18,at
R6008 T20528 T20520 punct ", ",detected
R6009 T20529 T20530 prep of,was
R6010 T20530 T20520 ccomp was,detected
R6011 T20531 T20529 pobj which,of
R6012 T20532 T20533 det the,species
R6013 T20533 T20530 nsubj species,was
R6014 T20534 T20533 advmod faster,species
R6015 T20535 T20533 amod migrating,species
R6016 T20536 T20530 acomp dominant,was
R6017 T20537 T20530 prep in,was
R6018 T20538 T20537 amod nuclear,in
R6019 T20539 T20530 cc and,was
R6020 T20540 T20541 det the,species
R6021 T20541 T20543 nsubj species,was
R6022 T20542 T20541 amod slower,species
R6023 T20543 T20530 conj was,was
R6024 T20544 T20543 acomp dominant,was
R6025 T20545 T20543 prep in,was
R6026 T20546 T20545 pobj cytoplasm,in
R6027 T20547 T20548 punct (,Figure
R6028 T20548 T20543 parataxis Figure,was
R6029 T20549 T20548 nummod 6,Figure
R6030 T20550 T20548 punct ),Figure
R6031 T20551 T20520 punct .,detected
R6032 T20553 T20554 det The,identity
R6033 T20554 T20555 nsubjpass identity,confirmed
R6034 T20556 T20554 prep of,identity
R6035 T20557 T20556 pobj both,of
R6036 T20558 T20554 prep as,identity
R6037 T20559 T20560 compound E2f3a,species
R6038 T20560 T20558 pobj species,as
R6039 T20561 T20555 auxpass was,confirmed
R6040 T20562 T20555 agent by,confirmed
R6041 T20563 T20564 poss their,absence
R6042 T20564 T20562 pobj absence,by
R6043 T20565 T20564 prep in,absence
R6044 T20566 T20567 det the,retina
R6045 T20567 T20565 pobj retina,in
R6046 T20568 T20567 compound P18,retina
R6047 T20569 T20570 compound E2f3a,KO
R6048 T20570 T20567 compound KO,retina
R6049 T20571 T20572 punct (,S8
R6050 T20572 T20555 parataxis S8,confirmed
R6051 T20573 T20572 compound Figure,S8
R6052 T20574 T20572 punct ),S8
R6053 T20575 T20555 punct .,confirmed
R6054 T20577 T20578 nsubj Analysis,showed
R6055 T20579 T20577 prep of,Analysis
R6056 T20580 T20579 pobj Pou4f2,of
R6057 T20581 T20580 punct ", ",Pou4f2
R6058 T20582 T20583 det a,factor
R6059 T20583 T20580 appos factor,Pou4f2
R6060 T20584 T20583 amod nuclear,factor
R6061 T20585 T20583 compound transcription,factor
R6062 T20586 T20583 acl expressed,factor
R6063 T20587 T20586 prep in,expressed
R6064 T20588 T20589 compound ganglion,cells
R6065 T20589 T20587 pobj cells,in
R6066 T20590 T20578 punct ", ",showed
R6067 T20591 T20592 mark that,contaminated
R6068 T20592 T20578 ccomp contaminated,showed
R6069 T20593 T20594 amod nuclear,proteins
R6070 T20594 T20592 nsubj proteins,contaminated
R6071 T20595 T20592 aux had,contaminated
R6072 T20596 T20592 neg not,contaminated
R6073 T20597 T20598 det the,fraction
R6074 T20598 T20592 dobj fraction,contaminated
R6075 T20599 T20598 amod cytoplasmic,fraction
R6076 T20600 T20578 punct ", ",showed
R6077 T20601 T20578 cc and,showed
R6078 T20602 T20603 nsubj analysis,confirmed
R6079 T20603 T20578 conj confirmed,showed
R6080 T20604 T20602 prep of,analysis
R6081 T20605 T20604 pobj Slc18a3,of
R6082 T20606 T20605 punct ", ",Slc18a3
R6083 T20607 T20608 det a,marker
R6084 T20608 T20605 appos marker,Slc18a3
R6085 T20609 T20608 amod cytoplasmic,marker
R6086 T20610 T20608 compound SAC,marker
R6087 T20611 T20603 punct ", ",confirmed
R6088 T20612 T20613 mark that,occurred
R6089 T20613 T20603 ccomp occurred,confirmed
R6090 T20614 T20615 det the,reverse
R6091 T20615 T20613 nsubj reverse,occurred
R6092 T20616 T20613 aux had,occurred
R6093 T20617 T20613 advmod also,occurred
R6094 T20618 T20613 neg not,occurred
R6095 T20619 T20620 punct (,Figure
R6096 T20620 T20613 parataxis Figure,occurred
R6097 T20621 T20620 nummod 6,Figure
R6098 T20622 T20620 punct ),Figure
R6099 T20623 T20603 punct .,confirmed
R6100 T20625 T20626 det These,data
R6101 T20626 T20627 nsubj data,show
R6102 T20628 T20627 punct ", ",show
R6103 T20629 T20630 prep to,for
R6104 T20630 T20627 prep for,show
R6105 T20631 T20632 poss our,knowledge
R6106 T20632 T20629 pobj knowledge,to
R6107 T20633 T20634 det the,time
R6108 T20634 T20630 pobj time,for
R6109 T20635 T20634 amod first,time
R6110 T20636 T20627 punct ", ",show
R6111 T20637 T20638 mark that,exhibit
R6112 T20638 T20627 ccomp exhibit,show
R6113 T20639 T20638 nsubj E2f3a,exhibit
R6114 T20640 T20639 cc and,E2f3a
R6115 T20641 T20639 conj E2f3b,E2f3a
R6116 T20642 T20643 amod distinct,patterns
R6117 T20643 T20638 dobj patterns,exhibit
R6118 T20644 T20643 prep of,patterns
R6119 T20645 T20646 amod subcellular,distribution
R6120 T20646 T20644 pobj distribution,of
R6121 T20647 T20638 punct ", ",exhibit
R6122 T20648 T20638 cc and,exhibit
R6123 T20649 T20638 conj raise,exhibit
R6124 T20650 T20651 det the,possibility
R6125 T20651 T20649 dobj possibility,raise
R6126 T20652 T20653 mark that,regulated
R6127 T20653 T20651 acl regulated,possibility
R6128 T20654 T20655 compound E2f3a,localization
R6129 T20655 T20653 nsubjpass localization,regulated
R6130 T20656 T20653 aux may,regulated
R6131 T20657 T20653 auxpass be,regulated
R6132 T20658 T20653 agent by,regulated
R6133 T20659 T20660 advmod as,yet
R6134 T20660 T20661 advmod yet,unknown
R6135 T20661 T20662 amod unknown,modifications
R6136 T20662 T20658 pobj modifications,by
R6137 T20663 T20662 amod post-translational,modifications
R6138 T20664 T20627 punct .,show