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PMC:1914394 / 11622-43093 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T6888 9-11 NN denotes Rb
T6889 12-21 VBZ denotes Regulates
T6890 22-30 NN denotes Division
T6891 31-34 CC denotes and
T6892 35-40 NN denotes Death
T6893 41-48 IN denotes through
T6894 49-53 NN denotes E2f1
T6895 53-159 sentence denotes We used the α-Cre transgene to delete floxed Rb exon 19 at embryonic day (E) 10 in peripheral retina [2].
T6896 54-56 PRP denotes We
T6897 57-61 VBD denotes used
T6898 62-65 DT denotes the
T6900 66-67 NN denotes α
T6902 67-68 HYPH denotes -
T6901 68-71 NN denotes Cre
T6899 72-81 NN denotes transgene
T6903 82-84 TO denotes to
T6904 85-91 VB denotes delete
T6905 92-98 VBN denotes floxed
T6907 99-101 NN denotes Rb
T6906 102-106 NN denotes exon
T6908 107-109 CD denotes 19
T6909 110-112 IN denotes at
T6910 113-122 JJ denotes embryonic
T6911 123-126 NN denotes day
T6912 127-128 -LRB- denotes (
T6913 128-129 NN denotes E
T6914 129-130 -RRB- denotes )
T6915 131-133 CD denotes 10
T6916 134-136 IN denotes in
T6917 137-147 JJ denotes peripheral
T6918 148-154 NN denotes retina
T6919 155-156 -LRB- denotes [
T6920 156-157 CD denotes 2
T6921 157-158 -RRB- denotes ]
T6922 158-159 . denotes .
T6923 159-291 sentence denotes RbloxP/loxP;α-Cre mice were bred with strains lacking E2f1 or E2f2 in the germ line, or a strain carrying a floxed E2f3 allele [5].
T6924 160-166 NN denotes RbloxP
T6926 166-167 HYPH denotes /
T6925 167-171 NN denotes loxP
T6928 171-172 : denotes ;
T6929 172-173 NN denotes α
T6931 173-174 HYPH denotes -
T6930 174-177 NN denotes Cre
T6927 178-182 NNS denotes mice
T6933 183-187 VBD denotes were
T6932 188-192 VBN denotes bred
T6934 193-197 IN denotes with
T6935 198-205 NNS denotes strains
T6936 206-213 VBG denotes lacking
T6937 214-218 NN denotes E2f1
T6938 219-221 CC denotes or
T6939 222-226 NN denotes E2f2
T6940 227-229 IN denotes in
T6941 230-233 DT denotes the
T6943 234-238 NN denotes germ
T6942 239-243 NN denotes line
T6944 243-245 , denotes ,
T6945 245-247 CC denotes or
T6946 248-249 DT denotes a
T6947 250-256 NN denotes strain
T6948 257-265 VBG denotes carrying
T6949 266-267 DT denotes a
T6951 268-274 VBN denotes floxed
T6952 275-279 NN denotes E2f3
T6950 280-286 NN denotes allele
T6953 287-288 -LRB- denotes [
T6954 288-289 CD denotes 5
T6955 289-290 -RRB- denotes ]
T6956 290-291 . denotes .
T6957 291-483 sentence denotes RbloxP/loxP;E2f1+/− and RbloxP/loxP;E2f1+/−;α-Cre mice were bred to produce RbloxP/loxP;E2f1−/−;α-Cre mice at a frequency of 1/8 and littermate controls at the same or higher (1/4) frequency.
T6958 292-298 NN denotes RbloxP
T6960 298-299 HYPH denotes /
T6959 299-303 NN denotes loxP
T6962 303-304 : denotes ;
T6963 304-308 NN denotes E2f1
T6964 308-309 SYM denotes +
T6965 309-310 HYPH denotes /
T6966 310-311 SYM denotes −
T6967 312-315 CC denotes and
T6968 316-322 NN denotes RbloxP
T6970 322-323 HYPH denotes /
T6969 323-327 NN denotes loxP
T6971 327-328 : denotes ;
T6972 328-332 NN denotes E2f1
T6973 332-333 SYM denotes +
T6974 333-334 HYPH denotes /
T6975 334-335 SYM denotes −
T6976 335-336 : denotes ;
T6977 336-337 NN denotes α
T6979 337-338 HYPH denotes -
T6978 338-341 NN denotes Cre
T6961 342-346 NNS denotes mice
T6981 347-351 VBD denotes were
T6980 352-356 VBN denotes bred
T6982 357-359 TO denotes to
T6983 360-367 VB denotes produce
T6984 368-374 NN denotes RbloxP
T6986 374-375 HYPH denotes /
T6985 375-379 NN denotes loxP
T6988 379-380 : denotes ;
T6989 380-384 NN denotes E2f1
T6990 384-385 SYM denotes −
T6991 385-386 HYPH denotes /
T6992 386-387 SYM denotes −
T6993 387-388 : denotes ;
T6994 388-389 NN denotes α
T6996 389-390 HYPH denotes -
T6995 390-393 NN denotes Cre
T6987 394-398 NNS denotes mice
T6997 399-401 IN denotes at
T6998 402-403 DT denotes a
T6999 404-413 NN denotes frequency
T7000 414-416 IN denotes of
T7001 417-418 CD denotes 1
T7003 418-419 SYM denotes /
T7002 419-420 CD denotes 8
T7004 421-424 CC denotes and
T7005 425-435 NN denotes littermate
T7006 436-444 NNS denotes controls
T7007 445-447 IN denotes at
T7008 448-451 DT denotes the
T7009 452-456 JJ denotes same
T7010 457-459 CC denotes or
T7011 460-466 JJR denotes higher
T7013 467-468 -LRB- denotes (
T7014 468-469 CD denotes 1
T7015 469-470 SYM denotes /
T7016 470-471 CD denotes 4
T7017 471-472 -RRB- denotes )
T7012 473-482 NN denotes frequency
T7018 482-483 . denotes .
T7019 483-608 sentence denotes For simplicity we will refer to the RbloxP/loxP;E2f1−/−;α-Cre peripheral retina as the Rb/E2f1 double knockout (DKO) retina.
T7020 484-487 IN denotes For
T7022 488-498 NN denotes simplicity
T7023 499-501 PRP denotes we
T7024 502-506 MD denotes will
T7021 507-512 VB denotes refer
T7025 513-515 IN denotes to
T7026 516-519 DT denotes the
T7028 520-526 NN denotes RbloxP
T7030 526-527 HYPH denotes /
T7029 527-531 NN denotes loxP
T7031 531-532 : denotes ;
T7032 532-536 NN denotes E2f1
T7033 536-537 SYM denotes −
T7034 537-538 HYPH denotes /
T7035 538-539 SYM denotes −
T7036 539-540 : denotes ;
T7037 540-541 NN denotes α
T7039 541-542 HYPH denotes -
T7038 542-545 NN denotes Cre
T7040 546-556 JJ denotes peripheral
T7027 557-563 NN denotes retina
T7041 564-566 IN denotes as
T7042 567-570 DT denotes the
T7044 571-573 NN denotes Rb
T7046 573-574 HYPH denotes /
T7045 574-578 NN denotes E2f1
T7047 579-585 JJ denotes double
T7048 586-594 NN denotes knockout
T7049 595-596 -LRB- denotes (
T7050 596-599 NN denotes DKO
T7051 599-600 -RRB- denotes )
T7043 601-607 NN denotes retina
T7052 607-608 . denotes .
T7053 608-670 sentence denotes Similar strategies were employed in the case of E2f2 or E2f3.
T7054 609-616 JJ denotes Similar
T7055 617-627 NNS denotes strategies
T7057 628-632 VBD denotes were
T7056 633-641 VBN denotes employed
T7058 642-644 IN denotes in
T7059 645-648 DT denotes the
T7060 649-653 NN denotes case
T7061 654-656 IN denotes of
T7062 657-661 NN denotes E2f2
T7063 662-664 CC denotes or
T7064 665-669 NN denotes E2f3
T7065 669-670 . denotes .
T7066 670-781 sentence denotes Cre-mediated excision of Rb and E2f3 alleles in the retina was confirmed by PCR as described previously [2,5].
T7067 671-674 NN denotes Cre
T7069 674-675 HYPH denotes -
T7068 675-683 VBN denotes mediated
T7070 684-692 NN denotes excision
T7072 693-695 IN denotes of
T7073 696-698 NN denotes Rb
T7075 699-702 CC denotes and
T7076 703-707 NN denotes E2f3
T7074 708-715 NNS denotes alleles
T7077 716-718 IN denotes in
T7078 719-722 DT denotes the
T7079 723-729 NN denotes retina
T7080 730-733 VBD denotes was
T7071 734-743 VBN denotes confirmed
T7081 744-746 IN denotes by
T7082 747-750 NN denotes PCR
T7083 751-753 IN denotes as
T7084 754-763 VBN denotes described
T7085 764-774 RB denotes previously
T7086 775-776 -LRB- denotes [
T7088 776-777 CD denotes 2
T7089 777-778 , denotes ,
T7087 778-779 CD denotes 5
T7090 779-780 -RRB- denotes ]
T7091 780-781 . denotes .
T7092 781-972 sentence denotes To measure ectopic cell division, mice were pulse-labelled with bromodeoxyuridine (BrdU) 2 h before sacrifice and the peripheral retina analyzed for BrdU incorporation by immunofluorescence.
T7093 782-784 TO denotes To
T7094 785-792 VB denotes measure
T7096 793-800 JJ denotes ectopic
T7098 801-805 NN denotes cell
T7097 806-814 NN denotes division
T7099 814-816 , denotes ,
T7100 816-820 NNS denotes mice
T7101 821-825 VBD denotes were
T7102 826-831 NN denotes pulse
T7103 831-832 HYPH denotes -
T7095 832-840 VBN denotes labelled
T7104 841-845 IN denotes with
T7105 846-863 NN denotes bromodeoxyuridine
T7106 864-865 -LRB- denotes (
T7107 865-869 NN denotes BrdU
T7108 869-870 -RRB- denotes )
T7109 871-872 CD denotes 2
T7110 873-874 NN denotes h
T7111 875-881 IN denotes before
T7112 882-891 NN denotes sacrifice
T7113 892-895 CC denotes and
T7114 896-899 DT denotes the
T7116 900-910 JJ denotes peripheral
T7115 911-917 NN denotes retina
T7117 918-926 VBN denotes analyzed
T7118 927-930 IN denotes for
T7119 931-935 NN denotes BrdU
T7120 936-949 NN denotes incorporation
T7121 950-952 IN denotes by
T7122 953-971 NN denotes immunofluorescence
T7123 971-972 . denotes .
T7124 972-1092 sentence denotes As reported before [2,3], Rb KO retinas exhibited both spatial and temporal ectopic DNA synthesis (Figures 1C and S1A).
T7125 973-975 IN denotes As
T7126 976-984 VBN denotes reported
T7128 985-991 RB denotes before
T7129 992-993 -LRB- denotes [
T7131 993-994 CD denotes 2
T7132 994-995 , denotes ,
T7130 995-996 CD denotes 3
T7133 996-997 -RRB- denotes ]
T7134 997-999 DT denotes ,
T7135 999-1001 NN denotes Rb
T7136 1002-1004 NN denotes KO
T7137 1005-1012 NNS denotes retinas
T7127 1013-1022 VBD denotes exhibited
T7138 1023-1027 CC denotes both
T7139 1028-1035 JJ denotes spatial
T7141 1036-1039 CC denotes and
T7142 1040-1048 JJ denotes temporal
T7143 1049-1056 JJ denotes ectopic
T7144 1057-1060 NN denotes DNA
T7140 1061-1070 NN denotes synthesis
T7145 1071-1072 -LRB- denotes (
T7147 1072-1079 NNS denotes Figures
T7146 1080-1082 NN denotes 1C
T7148 1083-1086 CC denotes and
T7149 1087-1090 NN denotes S1A
T7150 1090-1091 -RRB- denotes )
T7151 1091-1092 . denotes .
T7152 1092-1351 sentence denotes This is easily detected at E14, E16, and postnatal day (P) 0 in the inner retina where abnormal BrdU+ ganglion and amacrine RTCs are located, or on the outermost region of the P0 retina, where BrdU+ photoreceptor RTCs reside (Figures S1A and S2, arrows) [2].
T7153 1093-1097 DT denotes This
T7155 1098-1100 VBZ denotes is
T7156 1101-1107 RB denotes easily
T7154 1108-1116 VBN denotes detected
T7157 1117-1119 IN denotes at
T7158 1120-1123 NN denotes E14
T7159 1123-1125 , denotes ,
T7160 1125-1128 NN denotes E16
T7161 1128-1130 , denotes ,
T7162 1130-1133 CC denotes and
T7163 1134-1143 JJ denotes postnatal
T7164 1144-1147 NN denotes day
T7165 1148-1149 -LRB- denotes (
T7166 1149-1150 NN denotes P
T7167 1150-1151 -RRB- denotes )
T7168 1152-1153 CD denotes 0
T7169 1154-1156 IN denotes in
T7170 1157-1160 DT denotes the
T7172 1161-1166 JJ denotes inner
T7171 1167-1173 NN denotes retina
T7173 1174-1179 WRB denotes where
T7175 1180-1188 JJ denotes abnormal
T7177 1189-1193 NN denotes BrdU
T7178 1193-1194 SYM denotes +
T7179 1195-1203 NN denotes ganglion
T7180 1204-1207 CC denotes and
T7181 1208-1216 JJ denotes amacrine
T7176 1217-1221 NNS denotes RTCs
T7182 1222-1225 VBP denotes are
T7174 1226-1233 VBN denotes located
T7183 1233-1235 , denotes ,
T7184 1235-1237 CC denotes or
T7185 1238-1240 IN denotes on
T7186 1241-1244 DT denotes the
T7188 1245-1254 JJ denotes outermost
T7187 1255-1261 NN denotes region
T7189 1262-1264 IN denotes of
T7190 1265-1268 DT denotes the
T7192 1269-1271 NN denotes P0
T7191 1272-1278 NN denotes retina
T7193 1278-1280 , denotes ,
T7194 1280-1285 WRB denotes where
T7196 1286-1290 NN denotes BrdU
T7198 1290-1291 SYM denotes +
T7199 1292-1305 NN denotes photoreceptor
T7197 1306-1310 NNS denotes RTCs
T7195 1311-1317 VBP denotes reside
T7200 1318-1319 -LRB- denotes (
T7202 1319-1326 NNS denotes Figures
T7203 1327-1330 NN denotes S1A
T7204 1331-1334 CC denotes and
T7205 1335-1337 NN denotes S2
T7206 1337-1339 , denotes ,
T7201 1339-1345 NNS denotes arrows
T7207 1345-1346 -RRB- denotes )
T7208 1347-1348 -LRB- denotes [
T7209 1348-1349 CD denotes 2
T7210 1349-1350 -RRB- denotes ]
T7211 1350-1351 . denotes .
T7212 1351-1498 sentence denotes Ectopic RTC division in Rb KO retinas is even more obvious at P8 or P18, when division is completed in wild-type (WT) retina (Figures 1C and S1A).
T7213 1352-1359 JJ denotes Ectopic
T7215 1360-1363 NN denotes RTC
T7214 1364-1372 NN denotes division
T7217 1373-1375 IN denotes in
T7218 1376-1378 NN denotes Rb
T7219 1379-1381 NN denotes KO
T7220 1382-1389 NNS denotes retinas
T7216 1390-1392 VBZ denotes is
T7221 1393-1397 RB denotes even
T7222 1398-1402 RBR denotes more
T7223 1403-1410 JJ denotes obvious
T7224 1411-1413 IN denotes at
T7225 1414-1416 NN denotes P8
T7226 1417-1419 CC denotes or
T7227 1420-1423 NN denotes P18
T7228 1423-1425 , denotes ,
T7229 1425-1429 WRB denotes when
T7231 1430-1438 NN denotes division
T7232 1439-1441 VBZ denotes is
T7230 1442-1451 VBN denotes completed
T7233 1452-1454 IN denotes in
T7234 1455-1459 JJ denotes wild
T7236 1459-1460 HYPH denotes -
T7235 1460-1464 NN denotes type
T7238 1465-1466 -LRB- denotes (
T7239 1466-1468 NN denotes WT
T7240 1468-1469 -RRB- denotes )
T7237 1470-1476 NN denotes retina
T7241 1477-1478 -LRB- denotes (
T7243 1478-1485 NNS denotes Figures
T7242 1486-1488 NN denotes 1C
T7244 1489-1492 CC denotes and
T7245 1493-1496 NN denotes S1A
T7246 1496-1497 -RRB- denotes )
T7247 1497-1498 . denotes .
T7248 1498-1703 sentence denotes Strikingly, the ectopically positioned S-phase cells at E14, E16, and P0 and all the abnormal division at P8 and P18 were completely suppressed in the Rb/E2f1 DKO retina (Figures 1C, 1E, 1F, S1A, and S2).
T7249 1499-1509 RB denotes Strikingly
T7251 1509-1511 , denotes ,
T7252 1511-1514 DT denotes the
T7254 1515-1526 RB denotes ectopically
T7255 1527-1537 VBN denotes positioned
T7256 1538-1539 NN denotes S
T7258 1539-1540 HYPH denotes -
T7257 1540-1545 NN denotes phase
T7253 1546-1551 NNS denotes cells
T7259 1552-1554 IN denotes at
T7260 1555-1558 NN denotes E14
T7261 1558-1560 , denotes ,
T7262 1560-1563 NN denotes E16
T7263 1563-1565 , denotes ,
T7264 1565-1568 CC denotes and
T7265 1569-1571 NN denotes P0
T7266 1572-1575 CC denotes and
T7267 1576-1579 PDT denotes all
T7269 1580-1583 DT denotes the
T7270 1584-1592 JJ denotes abnormal
T7268 1593-1601 NN denotes division
T7271 1602-1604 IN denotes at
T7272 1605-1607 NN denotes P8
T7273 1608-1611 CC denotes and
T7274 1612-1615 NN denotes P18
T7275 1616-1620 VBD denotes were
T7276 1621-1631 RB denotes completely
T7250 1632-1642 VBN denotes suppressed
T7277 1643-1645 IN denotes in
T7278 1646-1649 DT denotes the
T7280 1650-1652 NN denotes Rb
T7282 1652-1653 HYPH denotes /
T7281 1653-1657 NN denotes E2f1
T7283 1658-1661 NN denotes DKO
T7279 1662-1668 NN denotes retina
T7284 1669-1670 -LRB- denotes (
T7286 1670-1677 NNS denotes Figures
T7285 1678-1680 NN denotes 1C
T7287 1680-1682 , denotes ,
T7288 1682-1684 NN denotes 1E
T7289 1684-1686 , denotes ,
T7290 1686-1688 NN denotes 1F
T7291 1688-1690 , denotes ,
T7292 1690-1693 NN denotes S1A
T7293 1693-1695 , denotes ,
T7294 1695-1698 CC denotes and
T7295 1699-1701 NN denotes S2
T7296 1701-1702 -RRB- denotes )
T7297 1702-1703 . denotes .
T7298 1703-1784 sentence denotes In contrast, deletion of E2f2 or E2f3 had no effect at any stage of development.
T7299 1704-1706 IN denotes In
T7301 1707-1715 NN denotes contrast
T7302 1715-1717 , denotes ,
T7303 1717-1725 NN denotes deletion
T7304 1726-1728 IN denotes of
T7305 1729-1733 NN denotes E2f2
T7306 1734-1736 CC denotes or
T7307 1737-1741 NN denotes E2f3
T7300 1742-1745 VBD denotes had
T7308 1746-1748 DT denotes no
T7309 1749-1755 NN denotes effect
T7310 1756-1758 IN denotes at
T7311 1759-1762 DT denotes any
T7312 1763-1768 NN denotes stage
T7313 1769-1771 IN denotes of
T7314 1772-1783 NN denotes development
T7315 1783-1784 . denotes .
T7316 1784-1955 sentence denotes Analysis of mitotic cells with anti–phosphohistone 3 (PH3)–specific antibodies confirmed that loss of E2f1, but not E2f2 or E2f3, suppressed ectopic division (Figure S3).
T7317 1785-1793 NN denotes Analysis
T7319 1794-1796 IN denotes of
T7320 1797-1804 JJ denotes mitotic
T7321 1805-1810 NNS denotes cells
T7322 1811-1815 IN denotes with
T7323 1816-1835 NN denotes anti–phosphohistone
T7325 1836-1837 CD denotes 3
T7326 1838-1839 -LRB- denotes (
T7327 1839-1842 NN denotes PH3
T7328 1842-1843 -RRB- denotes )
T7329 1843-1844 HYPH denotes –
T7324 1844-1852 JJ denotes specific
T7330 1853-1863 NNS denotes antibodies
T7318 1864-1873 VBD denotes confirmed
T7331 1874-1878 IN denotes that
T7333 1879-1883 NN denotes loss
T7334 1884-1886 IN denotes of
T7335 1887-1891 NN denotes E2f1
T7336 1891-1893 , denotes ,
T7337 1893-1896 CC denotes but
T7338 1897-1900 RB denotes not
T7339 1901-1905 NN denotes E2f2
T7340 1906-1908 CC denotes or
T7341 1909-1913 NN denotes E2f3
T7342 1913-1915 , denotes ,
T7332 1915-1925 VBD denotes suppressed
T7343 1926-1933 JJ denotes ectopic
T7344 1934-1942 NN denotes division
T7345 1943-1944 -LRB- denotes (
T7347 1944-1950 NN denotes Figure
T7346 1951-1953 NN denotes S3
T7348 1953-1954 -RRB- denotes )
T7349 1954-1955 . denotes .
T7350 1955-2170 sentence denotes Deleting one E2f1 allele partially suppressed ectopic S-phase and mitosis in Rb KO RTCs (Figures 1C, 1E, 1F, S1A, S2, and S3), suggesting that E2f1 drives ectopic division in Rb KO RTCs in a dose-dependent fashion.
T7351 1956-1964 VBG denotes Deleting
T7353 1965-1968 CD denotes one
T7355 1969-1973 NN denotes E2f1
T7354 1974-1980 NN denotes allele
T7356 1981-1990 RB denotes partially
T7352 1991-2001 VBD denotes suppressed
T7357 2002-2009 JJ denotes ectopic
T7359 2010-2011 NN denotes S
T7360 2011-2012 HYPH denotes -
T7358 2012-2017 NN denotes phase
T7361 2018-2021 CC denotes and
T7362 2022-2029 NN denotes mitosis
T7363 2030-2032 IN denotes in
T7364 2033-2035 NN denotes Rb
T7365 2036-2038 NN denotes KO
T7366 2039-2043 NNS denotes RTCs
T7367 2044-2045 -LRB- denotes (
T7369 2045-2052 NNS denotes Figures
T7368 2053-2055 NN denotes 1C
T7370 2055-2057 , denotes ,
T7371 2057-2059 NN denotes 1E
T7372 2059-2061 , denotes ,
T7373 2061-2063 NN denotes 1F
T7374 2063-2065 , denotes ,
T7375 2065-2068 NN denotes S1A
T7376 2068-2070 , denotes ,
T7377 2070-2072 NN denotes S2
T7378 2072-2074 , denotes ,
T7379 2074-2077 CC denotes and
T7380 2078-2080 NN denotes S3
T7381 2080-2081 -RRB- denotes )
T7382 2081-2083 , denotes ,
T7383 2083-2093 VBG denotes suggesting
T7384 2094-2098 IN denotes that
T7386 2099-2103 NN denotes E2f1
T7385 2104-2110 VBZ denotes drives
T7387 2111-2118 JJ denotes ectopic
T7388 2119-2127 NN denotes division
T7389 2128-2130 IN denotes in
T7390 2131-2133 NN denotes Rb
T7391 2134-2136 NN denotes KO
T7392 2137-2141 NNS denotes RTCs
T7393 2142-2144 IN denotes in
T7394 2145-2146 DT denotes a
T7396 2147-2151 NN denotes dose
T7398 2151-2152 HYPH denotes -
T7397 2152-2161 JJ denotes dependent
T7395 2162-2169 NN denotes fashion
T7399 2169-2170 . denotes .
T7400 2170-2338 sentence denotes These data contrast with previous findings in the lens and CNS of Rb KO embryos, where deletion of any activating E2f suppresses ectopic division to some extent [6–9].
T7401 2171-2176 DT denotes These
T7402 2177-2181 NNS denotes data
T7403 2182-2190 VBP denotes contrast
T7404 2191-2195 IN denotes with
T7405 2196-2204 JJ denotes previous
T7406 2205-2213 NNS denotes findings
T7407 2214-2216 IN denotes in
T7408 2217-2220 DT denotes the
T7409 2221-2225 NN denotes lens
T7410 2226-2229 CC denotes and
T7411 2230-2233 NN denotes CNS
T7412 2234-2236 IN denotes of
T7413 2237-2239 NN denotes Rb
T7414 2240-2242 NN denotes KO
T7415 2243-2250 NNS denotes embryos
T7416 2250-2252 , denotes ,
T7417 2252-2257 WRB denotes where
T7419 2258-2266 NN denotes deletion
T7420 2267-2269 IN denotes of
T7421 2270-2273 DT denotes any
T7423 2274-2284 VBG denotes activating
T7422 2285-2288 NN denotes E2f
T7418 2289-2299 VBZ denotes suppresses
T7424 2300-2307 JJ denotes ectopic
T7425 2308-2316 NN denotes division
T7426 2317-2319 IN denotes to
T7427 2320-2324 DT denotes some
T7428 2325-2331 NN denotes extent
T7429 2332-2333 -LRB- denotes [
T7430 2333-2334 CD denotes 6
T7431 2334-2335 SYM denotes –
T7432 2335-2336 CD denotes 9
T7433 2336-2337 -RRB- denotes ]
T7434 2337-2338 . denotes .
T7435 2338-2489 sentence denotes Loss of Rb in the retina results in considerable RTC apoptosis, eliminating most bipolar and ganglion cells as well as many rods (Figure 2A–2D) [2,3].
T7436 2339-2343 NN denotes Loss
T7438 2344-2346 IN denotes of
T7439 2347-2349 NN denotes Rb
T7440 2350-2352 IN denotes in
T7441 2353-2356 DT denotes the
T7442 2357-2363 NN denotes retina
T7437 2364-2371 VBZ denotes results
T7443 2372-2374 IN denotes in
T7444 2375-2387 JJ denotes considerable
T7446 2388-2391 NN denotes RTC
T7445 2392-2401 NN denotes apoptosis
T7447 2401-2403 , denotes ,
T7448 2403-2414 VBG denotes eliminating
T7449 2415-2419 JJS denotes most
T7451 2420-2427 JJ denotes bipolar
T7452 2428-2431 CC denotes and
T7453 2432-2440 NN denotes ganglion
T7450 2441-2446 NNS denotes cells
T7454 2447-2449 RB denotes as
T7456 2450-2454 RB denotes well
T7455 2455-2457 IN denotes as
T7457 2458-2462 JJ denotes many
T7458 2463-2467 NNS denotes rods
T7459 2468-2469 -LRB- denotes (
T7461 2469-2475 NN denotes Figure
T7460 2476-2478 NN denotes 2A
T7462 2478-2479 SYM denotes –
T7463 2479-2481 NN denotes 2D
T7464 2481-2482 -RRB- denotes )
T7465 2483-2484 -LRB- denotes [
T7467 2484-2485 CD denotes 2
T7468 2485-2486 , denotes ,
T7466 2486-2487 CD denotes 3
T7469 2487-2488 -RRB- denotes ]
T7470 2488-2489 . denotes .
T7471 2489-2745 sentence denotes The loss of Rb KO rods is evident from the thinner ONL, and the death of these cells as well as bipolar and ganglion neurons can be detected directly by double labelling for apoptotic and cell-type-specific markers [2] (M. P. and R. B., unpublished data).
T7472 2490-2493 DT denotes The
T7473 2494-2498 NN denotes loss
T7475 2499-2501 IN denotes of
T7476 2502-2504 NN denotes Rb
T7477 2505-2507 NN denotes KO
T7478 2508-2512 NNS denotes rods
T7474 2513-2515 VBZ denotes is
T7479 2516-2523 JJ denotes evident
T7480 2524-2528 IN denotes from
T7481 2529-2532 DT denotes the
T7483 2533-2540 JJ denotes thinner
T7482 2541-2544 NN denotes ONL
T7484 2544-2546 , denotes ,
T7485 2546-2549 CC denotes and
T7486 2550-2553 DT denotes the
T7487 2554-2559 NN denotes death
T7489 2560-2562 IN denotes of
T7490 2563-2568 DT denotes these
T7491 2569-2574 NNS denotes cells
T7492 2575-2577 RB denotes as
T7494 2578-2582 RB denotes well
T7493 2583-2585 IN denotes as
T7495 2586-2593 JJ denotes bipolar
T7497 2594-2597 CC denotes and
T7498 2598-2606 NN denotes ganglion
T7496 2607-2614 NNS denotes neurons
T7499 2615-2618 MD denotes can
T7500 2619-2621 VB denotes be
T7488 2622-2630 VBN denotes detected
T7501 2631-2639 RB denotes directly
T7502 2640-2642 IN denotes by
T7503 2643-2649 RB denotes double
T7504 2650-2659 VBG denotes labelling
T7505 2660-2663 IN denotes for
T7506 2664-2673 JJ denotes apoptotic
T7508 2674-2677 CC denotes and
T7509 2678-2682 NN denotes cell
T7511 2682-2683 HYPH denotes -
T7510 2683-2687 NN denotes type
T7513 2687-2688 HYPH denotes -
T7512 2688-2696 JJ denotes specific
T7507 2697-2704 NNS denotes markers
T7514 2705-2706 -LRB- denotes [
T7515 2706-2707 CD denotes 2
T7516 2707-2708 -RRB- denotes ]
T7517 2709-2710 -LRB- denotes (
T7518 2710-2712 NNP denotes M.
T7519 2713-2715 NNP denotes P.
T7520 2716-2719 CC denotes and
T7521 2720-2722 NNP denotes R.
T7522 2723-2725 NNP denotes B.
T7523 2725-2727 , denotes ,
T7524 2727-2738 JJ denotes unpublished
T7525 2739-2743 NNS denotes data
T7526 2743-2744 -RRB- denotes )
T7527 2744-2745 . denotes .
T7528 2745-2871 sentence denotes Loss of peripheral Rb KO ganglion cells is also evident from thinning of the optic nerve (D. C. and R. B., unpublished data).
T7529 2746-2750 NN denotes Loss
T7531 2751-2753 IN denotes of
T7532 2754-2764 JJ denotes peripheral
T7534 2765-2767 NN denotes Rb
T7535 2768-2770 NN denotes KO
T7536 2771-2779 NN denotes ganglion
T7533 2780-2785 NNS denotes cells
T7530 2786-2788 VBZ denotes is
T7537 2789-2793 RB denotes also
T7538 2794-2801 JJ denotes evident
T7539 2802-2806 IN denotes from
T7540 2807-2815 VBG denotes thinning
T7541 2816-2818 IN denotes of
T7542 2819-2822 DT denotes the
T7544 2823-2828 JJ denotes optic
T7543 2829-2834 NN denotes nerve
T7545 2835-2836 -LRB- denotes (
T7546 2836-2838 NNP denotes D.
T7547 2839-2841 NNP denotes C.
T7548 2842-2845 CC denotes and
T7549 2846-2848 NNP denotes R.
T7550 2849-2851 NNP denotes B.
T7551 2851-2853 , denotes ,
T7552 2853-2864 JJ denotes unpublished
T7553 2865-2869 NNS denotes data
T7554 2869-2870 -RRB- denotes )
T7555 2870-2871 . denotes .
T7556 2871-2995 sentence denotes Deleting E2f1, but not E2f2 or E2f3, blocked this ectopic cell death in a dose-dependent fashion (Figures 1D, 1G, and S1B).
T7557 2872-2880 VBG denotes Deleting
T7559 2881-2885 NN denotes E2f1
T7560 2885-2887 , denotes ,
T7561 2887-2890 CC denotes but
T7562 2891-2894 RB denotes not
T7563 2895-2899 NN denotes E2f2
T7564 2900-2902 CC denotes or
T7565 2903-2907 NN denotes E2f3
T7566 2907-2909 , denotes ,
T7558 2909-2916 VBD denotes blocked
T7567 2917-2921 DT denotes this
T7569 2922-2929 JJ denotes ectopic
T7570 2930-2934 NN denotes cell
T7568 2935-2940 NN denotes death
T7571 2941-2943 IN denotes in
T7572 2944-2945 DT denotes a
T7574 2946-2950 NN denotes dose
T7576 2950-2951 HYPH denotes -
T7575 2951-2960 JJ denotes dependent
T7573 2961-2968 NN denotes fashion
T7577 2969-2970 -LRB- denotes (
T7579 2970-2977 NNS denotes Figures
T7578 2978-2980 NN denotes 1D
T7580 2980-2982 , denotes ,
T7581 2982-2984 NN denotes 1G
T7582 2984-2986 , denotes ,
T7583 2986-2989 CC denotes and
T7584 2990-2993 NN denotes S1B
T7585 2993-2994 -RRB- denotes )
T7586 2994-2995 . denotes .
T7587 2995-4510 sentence denotes Figure 2 E2f1 Deletion Rescues Ganglion, Rod, and Bipolar Cells in the Rb KO Retina (A) Horizontal retinal sections from mice of the indicated ages and genotypes were stained for nuclei (DAPI, blue) and markers that detect ganglion cells (Pou4f2, red), rods and cones (Sag [rod arrestin], green), and rod bipolar cells (Prkca, green, and Cabp5, red). Scale bars are 50 μm. (B) Quantification of Pou4f2+ ganglion cells. (C) Quantification of Prkca+ and Cabp5+ bipolar cells. (D) Thickness of the ONL, which represents the number of rods. Error bars represent SD of measurements from three animals, and asterisks indicate a significant difference between retinas of WT and the indicated genotypes, unless indicated otherwise by connecting lines (*, p < 0.05; **; p < 0.01; ANOVA and Tukey HSD test). (E and F) ERGs were recorded from the indicated genotypes under dark-adapted (scotopic) conditions, and (E) intensity series and (F) b-wave amplitudes as a function of the logarithm of the flash intensity were determined. (F) Further illustrates that the relative influence of the mutations on the photoreceptors (indicated by the saturated a-wave amplitude, right graph) was not substantially different from their effect on the b-wave response (dominated by the bipolars, left graph) at the same intensity of 10 cd·s/m2. To investigate the molecular mechanism that underlies the unique role of E2f1, we assessed the expression of known E2f targets as well as other genes that regulate the cell cycle and apoptosis.
T34416 3006-3010 NN denotes E2f1
T34417 3011-3019 NN denotes Deletion
T34418 3020-3027 VBZ denotes Rescues
T34419 3028-3036 NN denotes Ganglion
T34421 3036-3038 , denotes ,
T34422 3038-3041 NN denotes Rod
T34423 3041-3043 , denotes ,
T34424 3043-3046 CC denotes and
T34425 3047-3054 JJ denotes Bipolar
T34420 3055-3060 NNS denotes Cells
T34426 3061-3063 IN denotes in
T34427 3064-3067 DT denotes the
T34429 3068-3070 NN denotes Rb
T34430 3071-3073 NN denotes KO
T34428 3074-3080 NN denotes Retina
T34431 3080-3347 sentence denotes (A) Horizontal retinal sections from mice of the indicated ages and genotypes were stained for nuclei (DAPI, blue) and markers that detect ganglion cells (Pou4f2, red), rods and cones (Sag [rod arrestin], green), and rod bipolar cells (Prkca, green, and Cabp5, red).
T34432 3081-3082 -LRB- denotes (
T34433 3082-3083 LS denotes A
T34435 3083-3084 -RRB- denotes )
T34436 3085-3095 JJ denotes Horizontal
T34438 3096-3103 JJ denotes retinal
T34437 3104-3112 NNS denotes sections
T34439 3113-3117 IN denotes from
T34440 3118-3122 NNS denotes mice
T34441 3123-3125 IN denotes of
T34442 3126-3129 DT denotes the
T34444 3130-3139 VBN denotes indicated
T34443 3140-3144 NNS denotes ages
T34445 3145-3148 CC denotes and
T34446 3149-3158 NNS denotes genotypes
T34447 3159-3163 VBD denotes were
T34434 3164-3171 VBN denotes stained
T34448 3172-3175 IN denotes for
T34449 3176-3182 NNS denotes nuclei
T34450 3183-3184 -LRB- denotes (
T34452 3184-3188 NN denotes DAPI
T34453 3188-3190 , denotes ,
T34451 3190-3194 JJ denotes blue
T34454 3194-3195 -RRB- denotes )
T34455 3196-3199 CC denotes and
T34456 3200-3207 NNS denotes markers
T34457 3208-3212 WDT denotes that
T34458 3213-3219 VBP denotes detect
T34459 3220-3228 NN denotes ganglion
T34460 3229-3234 NNS denotes cells
T34461 3235-3236 -LRB- denotes (
T34463 3236-3242 NN denotes Pou4f2
T34464 3242-3244 , denotes ,
T34462 3244-3247 JJ denotes red
T34465 3247-3248 -RRB- denotes )
T34466 3248-3250 , denotes ,
T34467 3250-3254 NNS denotes rods
T34468 3255-3258 CC denotes and
T34469 3259-3264 NNS denotes cones
T34470 3265-3266 -LRB- denotes (
T34472 3266-3269 NN denotes Sag
T34473 3270-3271 -LRB- denotes [
T34474 3271-3274 NN denotes rod
T34475 3275-3283 NN denotes arrestin
T34476 3283-3284 -RRB- denotes ]
T34477 3284-3286 , denotes ,
T34471 3286-3291 JJ denotes green
T34478 3291-3292 -RRB- denotes )
T34479 3292-3294 , denotes ,
T34480 3294-3297 CC denotes and
T34481 3298-3301 NN denotes rod
T34483 3302-3309 JJ denotes bipolar
T34482 3310-3315 NNS denotes cells
T34484 3316-3317 -LRB- denotes (
T34486 3317-3322 NN denotes Prkca
T34487 3322-3324 , denotes ,
T34485 3324-3329 JJ denotes green
T34488 3329-3331 , denotes ,
T34489 3331-3334 CC denotes and
T34490 3335-3340 NN denotes Cabp5
T34492 3340-3342 , denotes ,
T34491 3342-3345 JJ denotes red
T34493 3345-3346 -RRB- denotes )
T34494 3346-3347 . denotes .
T34495 3347-3369 sentence denotes Scale bars are 50 μm.
T34496 3348-3353 NN denotes Scale
T34497 3354-3358 NNS denotes bars
T34498 3359-3362 VBP denotes are
T34499 3363-3365 CD denotes 50
T34500 3366-3368 NN denotes μm
T34501 3368-3369 . denotes .
T34502 3369-3415 sentence denotes (B) Quantification of Pou4f2+ ganglion cells.
T34503 3370-3371 -LRB- denotes (
T34504 3371-3372 LS denotes B
T34506 3372-3373 -RRB- denotes )
T34505 3374-3388 NN denotes Quantification
T34507 3389-3391 IN denotes of
T34508 3392-3398 NN denotes Pou4f2
T34510 3398-3399 SYM denotes +
T34511 3400-3408 NN denotes ganglion
T34509 3409-3414 NNS denotes cells
T34512 3414-3415 . denotes .
T34513 3415-3470 sentence denotes (C) Quantification of Prkca+ and Cabp5+ bipolar cells.
T34514 3416-3417 -LRB- denotes (
T34515 3417-3418 LS denotes C
T34517 3418-3419 -RRB- denotes )
T34516 3420-3434 NN denotes Quantification
T34518 3435-3437 IN denotes of
T34519 3438-3443 NN denotes Prkca
T34521 3443-3444 SYM denotes +
T34522 3445-3448 CC denotes and
T34523 3449-3454 NN denotes Cabp5
T34524 3454-3455 SYM denotes +
T34525 3456-3463 JJ denotes bipolar
T34520 3464-3469 NNS denotes cells
T34526 3469-3470 . denotes .
T34527 3470-3533 sentence denotes (D) Thickness of the ONL, which represents the number of rods.
T34528 3471-3472 -LRB- denotes (
T34529 3472-3473 LS denotes D
T34531 3473-3474 -RRB- denotes )
T34530 3475-3484 NN denotes Thickness
T34532 3485-3487 IN denotes of
T34533 3488-3491 DT denotes the
T34534 3492-3495 NN denotes ONL
T34535 3495-3497 , denotes ,
T34536 3497-3502 WDT denotes which
T34537 3503-3513 VBZ denotes represents
T34538 3514-3517 DT denotes the
T34539 3518-3524 NN denotes number
T34540 3525-3527 IN denotes of
T34541 3528-3532 NNS denotes rods
T34542 3532-3533 . denotes .
T34543 3533-3794 sentence denotes Error bars represent SD of measurements from three animals, and asterisks indicate a significant difference between retinas of WT and the indicated genotypes, unless indicated otherwise by connecting lines (*, p < 0.05; **; p < 0.01; ANOVA and Tukey HSD test).
T34544 3534-3539 NN denotes Error
T34545 3540-3544 NNS denotes bars
T34546 3545-3554 VBP denotes represent
T34547 3555-3557 NN denotes SD
T34548 3558-3560 IN denotes of
T34549 3561-3573 NNS denotes measurements
T34550 3574-3578 IN denotes from
T34551 3579-3584 CD denotes three
T34552 3585-3592 NNS denotes animals
T34553 3592-3594 , denotes ,
T34554 3594-3597 CC denotes and
T34555 3598-3607 NNS denotes asterisks
T34556 3608-3616 VBP denotes indicate
T34557 3617-3618 DT denotes a
T34559 3619-3630 JJ denotes significant
T34558 3631-3641 NN denotes difference
T34560 3642-3649 IN denotes between
T34561 3650-3657 NNS denotes retinas
T34562 3658-3660 IN denotes of
T34563 3661-3663 NN denotes WT
T34564 3664-3667 CC denotes and
T34565 3668-3671 DT denotes the
T34566 3672-3681 VBN denotes indicated
T34567 3682-3691 NNS denotes genotypes
T34568 3691-3693 , denotes ,
T34569 3693-3699 IN denotes unless
T34570 3700-3709 VBN denotes indicated
T34571 3710-3719 RB denotes otherwise
T34572 3720-3722 IN denotes by
T34573 3723-3733 VBG denotes connecting
T34574 3734-3739 NNS denotes lines
T34575 3740-3741 -LRB- denotes (
T34577 3741-3742 SYM denotes *
T34579 3742-3744 , denotes ,
T34580 3744-3745 NN denotes p
T34581 3746-3747 SYM denotes <
T34578 3748-3752 CD denotes 0.05
T34582 3752-3753 : denotes ;
T34583 3754-3756 SYM denotes **
T34585 3756-3757 : denotes ;
T34586 3758-3759 NN denotes p
T34587 3760-3761 SYM denotes <
T34584 3762-3766 CD denotes 0.01
T34588 3766-3767 : denotes ;
T34589 3768-3773 NN denotes ANOVA
T34590 3774-3777 CC denotes and
T34591 3778-3783 NNP denotes Tukey
T34592 3784-3787 NN denotes HSD
T34576 3788-3792 NN denotes test
T34593 3792-3793 -RRB- denotes )
T34594 3793-3794 . denotes .
T34595 3794-4016 sentence denotes (E and F) ERGs were recorded from the indicated genotypes under dark-adapted (scotopic) conditions, and (E) intensity series and (F) b-wave amplitudes as a function of the logarithm of the flash intensity were determined.
T34596 3795-3796 -LRB- denotes (
T34597 3796-3797 LS denotes E
T34599 3798-3801 CC denotes and
T34600 3802-3803 LS denotes F
T34601 3803-3804 -RRB- denotes )
T34602 3805-3809 NNS denotes ERGs
T34603 3810-3814 VBD denotes were
T34598 3815-3823 VBN denotes recorded
T34604 3824-3828 IN denotes from
T34605 3829-3832 DT denotes the
T34607 3833-3842 VBN denotes indicated
T34606 3843-3852 NNS denotes genotypes
T34608 3853-3858 IN denotes under
T34609 3859-3863 NN denotes dark
T34611 3863-3864 HYPH denotes -
T34610 3864-3871 VBN denotes adapted
T34613 3872-3873 -LRB- denotes (
T34614 3873-3881 JJ denotes scotopic
T34615 3881-3882 -RRB- denotes )
T34612 3883-3893 NNS denotes conditions
T34616 3893-3895 , denotes ,
T34617 3895-3898 CC denotes and
T34618 3899-3900 -LRB- denotes (
T34619 3900-3901 NN denotes E
T34621 3901-3902 -RRB- denotes )
T34622 3903-3912 NN denotes intensity
T34620 3913-3919 NN denotes series
T34624 3920-3923 CC denotes and
T34625 3924-3925 -LRB- denotes (
T34626 3925-3926 NN denotes F
T34628 3926-3927 -RRB- denotes )
T34629 3928-3929 NN denotes b
T34631 3929-3930 HYPH denotes -
T34630 3930-3934 NN denotes wave
T34627 3935-3945 NNS denotes amplitudes
T34632 3946-3948 IN denotes as
T34633 3949-3950 DT denotes a
T34634 3951-3959 NN denotes function
T34635 3960-3962 IN denotes of
T34636 3963-3966 DT denotes the
T34637 3967-3976 NN denotes logarithm
T34638 3977-3979 IN denotes of
T34639 3980-3983 DT denotes the
T34641 3984-3989 NN denotes flash
T34640 3990-3999 NN denotes intensity
T34642 4000-4004 VBD denotes were
T34623 4005-4015 VBN denotes determined
T34643 4015-4016 . denotes .
T34644 4016-4316 sentence denotes (F) Further illustrates that the relative influence of the mutations on the photoreceptors (indicated by the saturated a-wave amplitude, right graph) was not substantially different from their effect on the b-wave response (dominated by the bipolars, left graph) at the same intensity of 10 cd·s/m2.
T34645 4017-4018 -LRB- denotes (
T34647 4018-4019 NN denotes F
T34648 4019-4020 -RRB- denotes )
T34649 4021-4028 RB denotes Further
T34646 4029-4040 VBZ denotes illustrates
T34650 4041-4045 IN denotes that
T34652 4046-4049 DT denotes the
T34654 4050-4058 JJ denotes relative
T34653 4059-4068 NN denotes influence
T34655 4069-4071 IN denotes of
T34656 4072-4075 DT denotes the
T34657 4076-4085 NNS denotes mutations
T34658 4086-4088 IN denotes on
T34659 4089-4092 DT denotes the
T34660 4093-4107 NNS denotes photoreceptors
T34661 4108-4109 -LRB- denotes (
T34662 4109-4118 VBN denotes indicated
T34663 4119-4121 IN denotes by
T34664 4122-4125 DT denotes the
T34666 4126-4135 VBN denotes saturated
T34667 4136-4137 NN denotes a
T34669 4137-4138 HYPH denotes -
T34668 4138-4142 NN denotes wave
T34665 4143-4152 NN denotes amplitude
T34670 4152-4154 , denotes ,
T34672 4154-4159 JJ denotes right
T34671 4160-4165 NN denotes graph
T34673 4165-4166 -RRB- denotes )
T34651 4167-4170 VBD denotes was
T34674 4171-4174 RB denotes not
T34675 4175-4188 RB denotes substantially
T34676 4189-4198 JJ denotes different
T34677 4199-4203 IN denotes from
T34678 4204-4209 PRP$ denotes their
T34679 4210-4216 NN denotes effect
T34680 4217-4219 IN denotes on
T34681 4220-4223 DT denotes the
T34683 4224-4225 NN denotes b
T34685 4225-4226 HYPH denotes -
T34684 4226-4230 NN denotes wave
T34682 4231-4239 NN denotes response
T34686 4240-4241 -LRB- denotes (
T34687 4241-4250 VBN denotes dominated
T34688 4251-4253 IN denotes by
T34689 4254-4257 DT denotes the
T34690 4258-4266 NNS denotes bipolars
T34691 4266-4268 , denotes ,
T34693 4268-4272 JJ denotes left
T34692 4273-4278 NN denotes graph
T34694 4278-4279 -RRB- denotes )
T34695 4280-4282 IN denotes at
T34696 4283-4286 DT denotes the
T34698 4287-4291 JJ denotes same
T34697 4292-4301 NN denotes intensity
T34699 4302-4304 IN denotes of
T34700 4305-4307 CD denotes 10
T34702 4308-4310 NN denotes cd
T34703 4310-4311 SYM denotes ·
T34701 4311-4312 NN denotes s
T34704 4312-4313 SYM denotes /
T34705 4313-4315 NN denotes m2
T34706 4315-4316 . denotes .
T7588 4317-4319 TO denotes To
T7589 4320-4331 VB denotes investigate
T7591 4332-4335 DT denotes the
T7593 4336-4345 JJ denotes molecular
T7592 4346-4355 NN denotes mechanism
T7594 4356-4360 WDT denotes that
T7595 4361-4370 VBZ denotes underlies
T7596 4371-4374 DT denotes the
T7598 4375-4381 JJ denotes unique
T7597 4382-4386 NN denotes role
T7599 4387-4389 IN denotes of
T7600 4390-4394 NN denotes E2f1
T7601 4394-4396 , denotes ,
T7602 4396-4398 PRP denotes we
T7590 4399-4407 VBD denotes assessed
T7603 4408-4411 DT denotes the
T7604 4412-4422 NN denotes expression
T7605 4423-4425 IN denotes of
T7606 4426-4431 JJ denotes known
T7608 4432-4435 NN denotes E2f
T7607 4436-4443 NNS denotes targets
T7609 4444-4446 RB denotes as
T7611 4447-4451 RB denotes well
T7610 4452-4454 IN denotes as
T7612 4455-4460 JJ denotes other
T7613 4461-4466 NNS denotes genes
T7614 4467-4471 WDT denotes that
T7615 4472-4480 VBP denotes regulate
T7616 4481-4484 DT denotes the
T7618 4485-4489 NN denotes cell
T7617 4490-4495 NN denotes cycle
T7619 4496-4499 CC denotes and
T7620 4500-4509 NN denotes apoptosis
T7621 4509-4510 . denotes .
T7622 4510-4628 sentence denotes Numerous positive and negative cell cycle and apoptotic regulators were up-regulated in the Rb KO retina (Figure 1H).
T7623 4511-4519 JJ denotes Numerous
T7625 4520-4528 JJ denotes positive
T7626 4529-4532 CC denotes and
T7627 4533-4541 JJ denotes negative
T7628 4542-4546 NN denotes cell
T7624 4547-4552 NN denotes cycle
T7630 4553-4556 CC denotes and
T7631 4557-4566 JJ denotes apoptotic
T7632 4567-4577 NNS denotes regulators
T7633 4578-4582 VBD denotes were
T7634 4583-4585 RB denotes up
T7635 4585-4586 HYPH denotes -
T7629 4586-4595 VBN denotes regulated
T7636 4596-4598 IN denotes in
T7637 4599-4602 DT denotes the
T7639 4603-4605 NN denotes Rb
T7640 4606-4608 NN denotes KO
T7638 4609-4615 NN denotes retina
T7641 4616-4617 -LRB- denotes (
T7643 4617-4623 NN denotes Figure
T7642 4624-4626 NN denotes 1H
T7644 4626-4627 -RRB- denotes )
T7645 4627-4628 . denotes .
T7646 4628-4753 sentence denotes Among the E2f family, E2f1, E2f2, E2f3a, and E2f7 were induced following Rb loss, but E2f3b, E2f4, and E2f5 were unaffected.
T7647 4629-4634 IN denotes Among
T7649 4635-4638 DT denotes the
T7651 4639-4642 NN denotes E2f
T7650 4643-4649 NN denotes family
T7652 4649-4651 , denotes ,
T7653 4651-4655 NN denotes E2f1
T7654 4655-4657 , denotes ,
T7655 4657-4661 NN denotes E2f2
T7656 4661-4663 , denotes ,
T7657 4663-4668 NN denotes E2f3a
T7658 4668-4670 , denotes ,
T7659 4670-4673 CC denotes and
T7660 4674-4678 NN denotes E2f7
T7661 4679-4683 VBD denotes were
T7648 4684-4691 VBN denotes induced
T7662 4692-4701 VBG denotes following
T7663 4702-4704 NN denotes Rb
T7664 4705-4709 NN denotes loss
T7665 4709-4711 , denotes ,
T7666 4711-4714 CC denotes but
T7667 4715-4720 NN denotes E2f3b
T7669 4720-4722 , denotes ,
T7670 4722-4726 NN denotes E2f4
T7671 4726-4728 , denotes ,
T7672 4728-4731 CC denotes and
T7673 4732-4736 NN denotes E2f5
T7668 4737-4741 VBD denotes were
T7674 4742-4752 JJ denotes unaffected
T7675 4752-4753 . denotes .
T7676 4753-4943 sentence denotes Consistent with the BrdU and terminal dUTP nick-end labelling (TUNEL) analyses, E2f1 deletion specifically reversed all these molecular defects, but E2f3 deletion had no effect (Figure 1H).
T7677 4754-4764 JJ denotes Consistent
T7679 4765-4769 IN denotes with
T7680 4770-4773 DT denotes the
T7682 4774-4778 NN denotes BrdU
T7683 4779-4782 CC denotes and
T7684 4783-4791 JJ denotes terminal
T7686 4792-4796 NN denotes dUTP
T7687 4797-4801 NN denotes nick
T7688 4801-4802 HYPH denotes -
T7689 4802-4805 NN denotes end
T7685 4806-4815 NN denotes labelling
T7690 4816-4817 -LRB- denotes (
T7691 4817-4822 NN denotes TUNEL
T7692 4822-4823 -RRB- denotes )
T7681 4824-4832 NNS denotes analyses
T7693 4832-4834 , denotes ,
T7694 4834-4838 NN denotes E2f1
T7695 4839-4847 NN denotes deletion
T7696 4848-4860 RB denotes specifically
T7678 4861-4869 VBD denotes reversed
T7697 4870-4873 PDT denotes all
T7699 4874-4879 DT denotes these
T7700 4880-4889 JJ denotes molecular
T7698 4890-4897 NNS denotes defects
T7701 4897-4899 , denotes ,
T7702 4899-4902 CC denotes but
T7703 4903-4907 NN denotes E2f3
T7704 4908-4916 NN denotes deletion
T7705 4917-4920 VBD denotes had
T7706 4921-4923 DT denotes no
T7707 4924-4930 NN denotes effect
T7708 4931-4932 -LRB- denotes (
T7710 4932-4938 NN denotes Figure
T7709 4939-4941 NN denotes 1H
T7711 4941-4942 -RRB- denotes )
T7712 4942-4943 . denotes .
T9110 4945-4951 JJ denotes Normal
T9111 4952-4967 NN denotes Differentiation
T9112 4968-4970 IN denotes in
T9113 4971-4974 DT denotes the
T9115 4975-4977 NN denotes Rb
T9117 4977-4978 HYPH denotes /
T9116 4978-4982 NN denotes E2f1
T9118 4983-4986 NNP denotes DKO
T9114 4987-4993 NN denotes Retina
T9119 4993-5207 sentence denotes Because E2f1 deletion blocks abnormal division and death in the Rb KO retina, the Rb/E2f1 DKO retina provided a unique opportunity to evaluate whether Rb controls differentiation independent of cell cycle effects.
T9120 4994-5001 IN denotes Because
T9122 5002-5006 NN denotes E2f1
T9123 5007-5015 NN denotes deletion
T9121 5016-5022 VBZ denotes blocks
T9125 5023-5031 JJ denotes abnormal
T9126 5032-5040 NN denotes division
T9127 5041-5044 CC denotes and
T9128 5045-5050 NN denotes death
T9129 5051-5053 IN denotes in
T9130 5054-5057 DT denotes the
T9132 5058-5060 NN denotes Rb
T9133 5061-5063 NN denotes KO
T9131 5064-5070 NN denotes retina
T9134 5070-5072 , denotes ,
T9135 5072-5075 DT denotes the
T9137 5076-5078 NN denotes Rb
T9139 5078-5079 HYPH denotes /
T9138 5079-5083 NN denotes E2f1
T9140 5084-5087 NN denotes DKO
T9136 5088-5094 NN denotes retina
T9124 5095-5103 VBD denotes provided
T9141 5104-5105 DT denotes a
T9143 5106-5112 JJ denotes unique
T9142 5113-5124 NN denotes opportunity
T9144 5125-5127 TO denotes to
T9145 5128-5136 VB denotes evaluate
T9146 5137-5144 IN denotes whether
T9148 5145-5147 NN denotes Rb
T9147 5148-5156 VBZ denotes controls
T9149 5157-5172 NN denotes differentiation
T9150 5173-5184 JJ denotes independent
T9151 5185-5187 IN denotes of
T9152 5188-5192 NN denotes cell
T9153 5193-5198 NN denotes cycle
T9154 5199-5206 NNS denotes effects
T9155 5206-5207 . denotes .
T9156 5207-5376 sentence denotes The Rb/E2f1 DKO retina had many Sag+ (S-antigen/rod arrestin) photoreceptors, Pou4f2+ (Brn3b) ganglion cells, and numerous Prkca+/Cabp5+ bipolar neurons (Figure 2A–2D).
T9157 5208-5211 DT denotes The
T9159 5212-5214 NN denotes Rb
T9161 5214-5215 HYPH denotes /
T9160 5215-5219 NN denotes E2f1
T9162 5220-5223 NN denotes DKO
T9158 5224-5230 NN denotes retina
T9163 5231-5234 VBD denotes had
T9164 5235-5239 JJ denotes many
T9166 5240-5243 NN denotes Sag
T9167 5243-5244 SYM denotes +
T9168 5245-5246 -LRB- denotes (
T9169 5246-5247 NN denotes S
T9171 5247-5248 HYPH denotes -
T9172 5248-5255 NN denotes antigen
T9173 5255-5256 HYPH denotes /
T9174 5256-5259 NN denotes rod
T9170 5260-5268 NN denotes arrestin
T9175 5268-5269 -RRB- denotes )
T9165 5270-5284 NNS denotes photoreceptors
T9176 5284-5286 , denotes ,
T9177 5286-5292 NN denotes Pou4f2
T9179 5292-5293 SYM denotes +
T9180 5294-5295 -LRB- denotes (
T9181 5295-5300 NN denotes Brn3b
T9182 5300-5301 -RRB- denotes )
T9183 5302-5310 NN denotes ganglion
T9178 5311-5316 NNS denotes cells
T9184 5316-5318 , denotes ,
T9185 5318-5321 CC denotes and
T9186 5322-5330 JJ denotes numerous
T9188 5331-5336 NN denotes Prkca
T9189 5336-5337 SYM denotes +
T9190 5337-5338 HYPH denotes /
T9191 5338-5343 NN denotes Cabp5
T9192 5343-5344 SYM denotes +
T9193 5345-5352 JJ denotes bipolar
T9187 5353-5360 NNS denotes neurons
T9194 5361-5362 -LRB- denotes (
T9196 5362-5368 NN denotes Figure
T9195 5369-5371 NN denotes 2A
T9197 5371-5372 SYM denotes –
T9198 5372-5374 NN denotes 2D
T9199 5374-5375 -RRB- denotes )
T9200 5375-5376 . denotes .
T9201 5376-5475 sentence denotes In contrast, there was no such rescue of cell types in Rb/E2f2 or Rb/E2f3 DKO retinas (Figure S4).
T9202 5377-5379 IN denotes In
T9204 5380-5388 NN denotes contrast
T9205 5388-5390 , denotes ,
T9206 5390-5395 EX denotes there
T9203 5396-5399 VBD denotes was
T9207 5400-5402 DT denotes no
T9209 5403-5407 JJ denotes such
T9208 5408-5414 NN denotes rescue
T9210 5415-5417 IN denotes of
T9211 5418-5422 NN denotes cell
T9212 5423-5428 NNS denotes types
T9213 5429-5431 IN denotes in
T9214 5432-5434 NN denotes Rb
T9216 5434-5435 SYM denotes /
T9215 5435-5439 NN denotes E2f2
T9218 5440-5442 CC denotes or
T9219 5443-5445 NN denotes Rb
T9221 5445-5446 SYM denotes /
T9220 5446-5450 NN denotes E2f3
T9217 5451-5454 NN denotes DKO
T9222 5455-5462 NNS denotes retinas
T9223 5463-5464 -LRB- denotes (
T9225 5464-5470 NN denotes Figure
T9224 5471-5473 NN denotes S4
T9226 5473-5474 -RRB- denotes )
T9227 5474-5475 . denotes .
T9228 5475-5725 sentence denotes Analysis with general neuronal markers Mtap2 (MAP2) and Snap25, as well as other markers expressed in bipolar cells (Chx10, Rcvrn, Vsx1, Tacr3, and Atp2b1) and rod photoreceptors (Rho and Rcvrn) confirmed rescue of the Rb/E2f1 DKO retina (Table S1).
T9229 5476-5484 NN denotes Analysis
T9231 5485-5489 IN denotes with
T9232 5490-5497 JJ denotes general
T9234 5498-5506 JJ denotes neuronal
T9235 5507-5514 NNS denotes markers
T9233 5515-5520 NN denotes Mtap2
T9236 5521-5522 -LRB- denotes (
T9237 5522-5526 NN denotes MAP2
T9238 5526-5527 -RRB- denotes )
T9239 5528-5531 CC denotes and
T9240 5532-5538 NN denotes Snap25
T9241 5538-5540 , denotes ,
T9242 5540-5542 RB denotes as
T9244 5543-5547 RB denotes well
T9243 5548-5550 IN denotes as
T9245 5551-5556 JJ denotes other
T9246 5557-5564 NNS denotes markers
T9247 5565-5574 VBN denotes expressed
T9248 5575-5577 IN denotes in
T9249 5578-5585 JJ denotes bipolar
T9250 5586-5591 NNS denotes cells
T9251 5592-5593 -LRB- denotes (
T9252 5593-5598 NN denotes Chx10
T9253 5598-5600 , denotes ,
T9254 5600-5605 NN denotes Rcvrn
T9255 5605-5607 , denotes ,
T9256 5607-5611 NN denotes Vsx1
T9257 5611-5613 , denotes ,
T9258 5613-5618 NN denotes Tacr3
T9259 5618-5620 , denotes ,
T9260 5620-5623 CC denotes and
T9261 5624-5630 NN denotes Atp2b1
T9262 5630-5631 -RRB- denotes )
T9263 5632-5635 CC denotes and
T9264 5636-5639 NN denotes rod
T9265 5640-5654 NNS denotes photoreceptors
T9266 5655-5656 -LRB- denotes (
T9267 5656-5659 NN denotes Rho
T9268 5660-5663 CC denotes and
T9269 5664-5669 NN denotes Rcvrn
T9270 5669-5670 -RRB- denotes )
T9230 5671-5680 VBD denotes confirmed
T9271 5681-5687 NN denotes rescue
T9272 5688-5690 IN denotes of
T9273 5691-5694 DT denotes the
T9275 5695-5697 NN denotes Rb
T9277 5697-5698 HYPH denotes /
T9276 5698-5702 NN denotes E2f1
T9278 5703-5706 NN denotes DKO
T9274 5707-5713 NN denotes retina
T9279 5714-5715 -LRB- denotes (
T9281 5715-5720 NN denotes Table
T9280 5721-5723 NN denotes S1
T9282 5723-5724 -RRB- denotes )
T9283 5724-5725 . denotes .
T9284 5725-5798 sentence denotes Moreover, neurons exhibited the same complex morphology as in WT retina.
T9285 5726-5734 RB denotes Moreover
T9287 5734-5736 , denotes ,
T9288 5736-5743 NNS denotes neurons
T9286 5744-5753 VBD denotes exhibited
T9289 5754-5757 DT denotes the
T9291 5758-5762 JJ denotes same
T9292 5763-5770 NN denotes complex
T9290 5771-5781 NN denotes morphology
T9293 5782-5784 IN denotes as
T9294 5785-5787 IN denotes in
T9295 5788-5790 NN denotes WT
T9296 5791-5797 NN denotes retina
T9297 5797-5798 . denotes .
T9298 5798-5938 sentence denotes Bipolar cell bodies were located in the INL and had ascending and descending processes ending in the OPL and IPL, respectively (Figure 2A).
T9299 5799-5806 JJ denotes Bipolar
T9301 5807-5811 NN denotes cell
T9300 5812-5818 NNS denotes bodies
T9303 5819-5823 VBD denotes were
T9302 5824-5831 VBN denotes located
T9304 5832-5834 IN denotes in
T9305 5835-5838 DT denotes the
T9306 5839-5842 NN denotes INL
T9307 5843-5846 CC denotes and
T9308 5847-5850 VBD denotes had
T9309 5851-5860 VBG denotes ascending
T9311 5861-5864 CC denotes and
T9312 5865-5875 VBG denotes descending
T9310 5876-5885 NNS denotes processes
T9313 5886-5892 VBG denotes ending
T9314 5893-5895 IN denotes in
T9315 5896-5899 DT denotes the
T9316 5900-5903 NN denotes OPL
T9317 5904-5907 CC denotes and
T9318 5908-5911 NN denotes IPL
T9319 5911-5913 , denotes ,
T9320 5913-5925 RB denotes respectively
T9321 5926-5927 -LRB- denotes (
T9323 5927-5933 NN denotes Figure
T9322 5934-5936 NN denotes 2A
T9324 5936-5937 -RRB- denotes )
T9325 5937-5938 . denotes .
T9326 5938-6169 sentence denotes In addition, the Rb/E2f1 DKO retina had a healthy appearing ONL consisting of morphologically normal rods with descending processes ending in the OPL and ascending processes that terminated in inner and outer segments (Figure 2A).
T9327 5939-5941 IN denotes In
T9329 5942-5950 NN denotes addition
T9330 5950-5952 , denotes ,
T9331 5952-5955 DT denotes the
T9333 5956-5958 NN denotes Rb
T9335 5958-5959 HYPH denotes /
T9334 5959-5963 NN denotes E2f1
T9336 5964-5967 NN denotes DKO
T9332 5968-5974 NN denotes retina
T9328 5975-5978 VBD denotes had
T9337 5979-5980 DT denotes a
T9339 5981-5988 JJ denotes healthy
T9340 5989-5998 VBG denotes appearing
T9338 5999-6002 NN denotes ONL
T9341 6003-6013 VBG denotes consisting
T9342 6014-6016 IN denotes of
T9343 6017-6032 RB denotes morphologically
T9344 6033-6039 JJ denotes normal
T9345 6040-6044 NNS denotes rods
T9346 6045-6049 IN denotes with
T9347 6050-6060 VBG denotes descending
T9348 6061-6070 NNS denotes processes
T9349 6071-6077 VBG denotes ending
T9350 6078-6080 IN denotes in
T9351 6081-6084 DT denotes the
T9352 6085-6088 NN denotes OPL
T9353 6089-6092 CC denotes and
T9354 6093-6102 VBG denotes ascending
T9355 6103-6112 NNS denotes processes
T9356 6113-6117 WDT denotes that
T9357 6118-6128 VBD denotes terminated
T9358 6129-6131 IN denotes in
T9359 6132-6137 JJ denotes inner
T9361 6138-6141 CC denotes and
T9362 6142-6147 JJ denotes outer
T9360 6148-6156 NNS denotes segments
T9363 6157-6158 -LRB- denotes (
T9365 6158-6164 NN denotes Figure
T9364 6165-6167 NN denotes 2A
T9366 6167-6168 -RRB- denotes )
T9367 6168-6169 . denotes .
T9368 6169-6310 sentence denotes It was suggested that Rb might regulate photoreceptor differentiation, possibly through rod-specific transcription factors (Figure 1B) [29].
T9369 6170-6172 PRP denotes It
T9371 6173-6176 VBD denotes was
T9370 6177-6186 VBN denotes suggested
T9372 6187-6191 IN denotes that
T9374 6192-6194 NN denotes Rb
T9375 6195-6200 MD denotes might
T9373 6201-6209 VB denotes regulate
T9376 6210-6223 NN denotes photoreceptor
T9377 6224-6239 NN denotes differentiation
T9378 6239-6241 , denotes ,
T9379 6241-6249 RB denotes possibly
T9380 6250-6257 IN denotes through
T9381 6258-6261 NN denotes rod
T9383 6261-6262 HYPH denotes -
T9382 6262-6270 JJ denotes specific
T9385 6271-6284 NN denotes transcription
T9384 6285-6292 NNS denotes factors
T9386 6293-6294 -LRB- denotes (
T9388 6294-6300 NN denotes Figure
T9387 6301-6303 NN denotes 1B
T9389 6303-6304 -RRB- denotes )
T9390 6305-6306 -LRB- denotes [
T9391 6306-6308 CD denotes 29
T9392 6308-6309 -RRB- denotes ]
T9393 6309-6310 . denotes .
T9394 6310-6477 sentence denotes However, if Rb does regulate photoreceptor differentiation, it does so by inhibiting E2f1, not by potentiating rod differentiation factors, such as Otx2, Crx, or Nrl.
T9395 6311-6318 RB denotes However
T9397 6318-6320 , denotes ,
T9398 6320-6322 IN denotes if
T9400 6323-6325 NN denotes Rb
T9401 6326-6330 VBZ denotes does
T9399 6331-6339 VB denotes regulate
T9402 6340-6353 NN denotes photoreceptor
T9403 6354-6369 NN denotes differentiation
T9404 6369-6371 , denotes ,
T9405 6371-6373 PRP denotes it
T9396 6374-6378 VBZ denotes does
T9406 6379-6381 RB denotes so
T9407 6382-6384 IN denotes by
T9408 6385-6395 VBG denotes inhibiting
T9409 6396-6400 NN denotes E2f1
T9410 6400-6402 , denotes ,
T9411 6402-6405 CC denotes not
T9412 6406-6408 IN denotes by
T9413 6409-6421 VBG denotes potentiating
T9414 6422-6425 NN denotes rod
T9415 6426-6441 NN denotes differentiation
T9416 6442-6449 NNS denotes factors
T9417 6449-6451 , denotes ,
T9418 6451-6455 JJ denotes such
T9419 6456-6458 IN denotes as
T9420 6459-6463 NN denotes Otx2
T9421 6463-6465 , denotes ,
T9422 6465-6468 NN denotes Crx
T9423 6468-6470 , denotes ,
T9424 6470-6472 CC denotes or
T9425 6473-6476 NN denotes Nrl
T9426 6476-6477 . denotes .
T9427 6477-6694 sentence denotes It is impossible to tell whether E2f1 perturbs differentiation directly, by affecting the expression of genes that modulate maturation, and/or indirectly through its effects on proliferation and survival (Figure 1B).
T9428 6478-6480 PRP denotes It
T9429 6481-6483 VBZ denotes is
T9430 6484-6494 JJ denotes impossible
T9431 6495-6497 TO denotes to
T9432 6498-6502 VB denotes tell
T9433 6503-6510 IN denotes whether
T9435 6511-6515 NN denotes E2f1
T9434 6516-6524 NNS denotes perturbs
T9436 6525-6540 NN denotes differentiation
T9437 6541-6549 RB denotes directly
T9438 6549-6551 , denotes ,
T9439 6551-6553 IN denotes by
T9440 6554-6563 VBG denotes affecting
T9441 6564-6567 DT denotes the
T9442 6568-6578 NN denotes expression
T9443 6579-6581 IN denotes of
T9444 6582-6587 NNS denotes genes
T9445 6588-6592 WDT denotes that
T9446 6593-6601 VBP denotes modulate
T9447 6602-6612 NN denotes maturation
T9448 6612-6614 , denotes ,
T9449 6614-6617 CC denotes and
T9450 6617-6618 HYPH denotes /
T9451 6618-6620 CC denotes or
T9452 6621-6631 RB denotes indirectly
T9454 6632-6639 IN denotes through
T9455 6640-6643 PRP$ denotes its
T9456 6644-6651 NNS denotes effects
T9457 6652-6654 IN denotes on
T9458 6655-6668 NN denotes proliferation
T9459 6669-6672 CC denotes and
T9460 6673-6681 NN denotes survival
T9461 6682-6683 -LRB- denotes (
T9462 6683-6689 NN denotes Figure
T9453 6690-6692 NN denotes 1B
T9463 6692-6693 -RRB- denotes )
T9464 6693-6694 . denotes .
T9465 6694-6857 sentence denotes As with ectopic division and apoptosis (Figure 1C and 1D), the rescue of Rb KO retinal bipolar, ganglion, and rod cells was dependent on E2f1 dose (Figure 2A–2D).
T9466 6695-6697 IN denotes As
T9468 6698-6702 IN denotes with
T9469 6703-6710 JJ denotes ectopic
T9470 6711-6719 NN denotes division
T9471 6720-6723 CC denotes and
T9472 6724-6733 NN denotes apoptosis
T9473 6734-6735 -LRB- denotes (
T9475 6735-6741 NN denotes Figure
T9474 6742-6744 NN denotes 1C
T9476 6745-6748 CC denotes and
T9477 6749-6751 NN denotes 1D
T9478 6751-6752 -RRB- denotes )
T9479 6752-6754 , denotes ,
T9480 6754-6757 DT denotes the
T9481 6758-6764 NN denotes rescue
T9482 6765-6767 IN denotes of
T9483 6768-6770 NN denotes Rb
T9484 6771-6773 NN denotes KO
T9486 6774-6781 NN denotes retinal
T9487 6782-6789 JJ denotes bipolar
T9488 6789-6791 , denotes ,
T9489 6791-6799 NN denotes ganglion
T9490 6799-6801 , denotes ,
T9491 6801-6804 CC denotes and
T9492 6805-6808 NN denotes rod
T9485 6809-6814 NNS denotes cells
T9467 6815-6818 VBD denotes was
T9493 6819-6828 JJ denotes dependent
T9494 6829-6831 IN denotes on
T9495 6832-6836 NN denotes E2f1
T9496 6837-6841 NN denotes dose
T9497 6842-6843 -LRB- denotes (
T9499 6843-6849 NN denotes Figure
T9498 6850-6852 NN denotes 2A
T9500 6852-6853 SYM denotes –
T9501 6853-6855 NN denotes 2D
T9502 6855-6856 -RRB- denotes )
T9503 6856-6857 . denotes .
T9504 6857-7052 sentence denotes Separate from its role in driving ectopic division of Rb KO RTCs, E2f1 also promotes normal RPC division since in its absence RPC proliferation drops ~2-fold (D. C. and R. B., unpublished data).
T9505 6858-6866 JJ denotes Separate
T9507 6867-6871 IN denotes from
T9508 6872-6875 PRP$ denotes its
T9509 6876-6880 NN denotes role
T9510 6881-6883 IN denotes in
T9511 6884-6891 VBG denotes driving
T9512 6892-6899 JJ denotes ectopic
T9513 6900-6908 NN denotes division
T9514 6909-6911 IN denotes of
T9515 6912-6914 NN denotes Rb
T9516 6915-6917 NN denotes KO
T9517 6918-6922 NNS denotes RTCs
T9518 6922-6924 , denotes ,
T9519 6924-6928 NN denotes E2f1
T9520 6929-6933 RB denotes also
T9506 6934-6942 VBZ denotes promotes
T9521 6943-6949 JJ denotes normal
T9523 6950-6953 NN denotes RPC
T9522 6954-6962 NN denotes division
T9524 6963-6968 IN denotes since
T9526 6969-6971 IN denotes in
T9527 6972-6975 PRP$ denotes its
T9528 6976-6983 NN denotes absence
T9529 6984-6987 NN denotes RPC
T9530 6988-7001 NN denotes proliferation
T9525 7002-7007 VBZ denotes drops
T9531 7008-7009 SYM denotes ~
T9532 7009-7010 CD denotes 2
T9533 7010-7011 HYPH denotes -
T9534 7011-7015 RB denotes fold
T9535 7016-7017 -LRB- denotes (
T9536 7017-7019 NNP denotes D.
T9537 7020-7022 NNP denotes C.
T9538 7023-7026 CC denotes and
T9539 7027-7029 NNP denotes R.
T9540 7030-7032 NNP denotes B.
T9541 7032-7034 , denotes ,
T9542 7034-7045 JJ denotes unpublished
T9543 7046-7050 NNS denotes data
T9544 7050-7051 -RRB- denotes )
T9545 7051-7052 . denotes .
T9546 7052-7331 sentence denotes This modest reduction of RPC numbers in the absence of E2f1 accounts for the slight reduction in the number of ganglion cells at P0, in the number of bipolar cells at P18 or P30, and in the thickness of the ONL at P18 or P30 in the E2f1 KO and Rb/E2f1 DKO retina (Figure 2B–2D).
T9547 7053-7057 DT denotes This
T9549 7058-7064 JJ denotes modest
T9548 7065-7074 NN denotes reduction
T9551 7075-7077 IN denotes of
T9552 7078-7081 NN denotes RPC
T9553 7082-7089 NNS denotes numbers
T9554 7090-7092 IN denotes in
T9555 7093-7096 DT denotes the
T9556 7097-7104 NN denotes absence
T9557 7105-7107 IN denotes of
T9558 7108-7112 NN denotes E2f1
T9550 7113-7121 VBZ denotes accounts
T9559 7122-7125 IN denotes for
T9560 7126-7129 DT denotes the
T9562 7130-7136 JJ denotes slight
T9561 7137-7146 NN denotes reduction
T9563 7147-7149 IN denotes in
T9564 7150-7153 DT denotes the
T9565 7154-7160 NN denotes number
T9566 7161-7163 IN denotes of
T9567 7164-7172 NN denotes ganglion
T9568 7173-7178 NNS denotes cells
T9569 7179-7181 IN denotes at
T9570 7182-7184 NN denotes P0
T9571 7184-7186 , denotes ,
T9572 7186-7188 IN denotes in
T9573 7189-7192 DT denotes the
T9574 7193-7199 NN denotes number
T9575 7200-7202 IN denotes of
T9576 7203-7210 JJ denotes bipolar
T9577 7211-7216 NNS denotes cells
T9578 7217-7219 IN denotes at
T9579 7220-7223 NN denotes P18
T9580 7224-7226 CC denotes or
T9581 7227-7230 NN denotes P30
T9582 7230-7232 , denotes ,
T9583 7232-7235 CC denotes and
T9584 7236-7238 IN denotes in
T9585 7239-7242 DT denotes the
T9586 7243-7252 NN denotes thickness
T9587 7253-7255 IN denotes of
T9588 7256-7259 DT denotes the
T9589 7260-7263 NN denotes ONL
T9590 7264-7266 IN denotes at
T9591 7267-7270 NN denotes P18
T9592 7271-7273 CC denotes or
T9593 7274-7277 NN denotes P30
T9594 7278-7280 IN denotes in
T9595 7281-7284 DT denotes the
T9597 7285-7289 NN denotes E2f1
T9598 7290-7292 NN denotes KO
T9599 7293-7296 CC denotes and
T9600 7297-7299 NN denotes Rb
T9602 7299-7300 HYPH denotes /
T9601 7300-7304 NN denotes E2f1
T9603 7305-7308 NN denotes DKO
T9596 7309-7315 NN denotes retina
T9604 7316-7317 -LRB- denotes (
T9606 7317-7323 NN denotes Figure
T9605 7324-7326 NN denotes 2B
T9607 7326-7327 SYM denotes –
T9608 7327-7329 NN denotes 2D
T9609 7329-7330 -RRB- denotes )
T9610 7330-7331 . denotes .
T9611 7331-7385 sentence denotes The morphology of E2f1 KO neurons was WT (Figure 2A).
T9612 7332-7335 DT denotes The
T9613 7336-7346 NN denotes morphology
T9615 7347-7349 IN denotes of
T9616 7350-7354 NN denotes E2f1
T9617 7355-7357 NN denotes KO
T9618 7358-7365 NNS denotes neurons
T9614 7366-7369 VBD denotes was
T9619 7370-7372 NN denotes WT
T9620 7373-7374 -LRB- denotes (
T9622 7374-7380 NN denotes Figure
T9621 7381-7383 NN denotes 2A
T9623 7383-7384 -RRB- denotes )
T9624 7384-7385 . denotes .
T9625 7385-7526 sentence denotes Despite a slight drop in absolute cell numbers, the proportion of Rb/E2f1 DKO and E2f1 KO bipolar cells was the same as WT (data not shown).
T9626 7386-7393 IN denotes Despite
T9628 7394-7395 DT denotes a
T9630 7396-7402 JJ denotes slight
T9629 7403-7407 NN denotes drop
T9631 7408-7410 IN denotes in
T9632 7411-7419 JJ denotes absolute
T9634 7420-7424 NN denotes cell
T9633 7425-7432 NNS denotes numbers
T9635 7432-7434 , denotes ,
T9636 7434-7437 DT denotes the
T9637 7438-7448 NN denotes proportion
T9638 7449-7451 IN denotes of
T9639 7452-7454 NN denotes Rb
T9641 7454-7455 HYPH denotes /
T9640 7455-7459 NN denotes E2f1
T9642 7460-7463 NN denotes DKO
T9644 7464-7467 CC denotes and
T9645 7468-7472 NN denotes E2f1
T9646 7473-7475 NN denotes KO
T9647 7476-7483 JJ denotes bipolar
T9643 7484-7489 NNS denotes cells
T9627 7490-7493 VBD denotes was
T9648 7494-7497 DT denotes the
T9649 7498-7502 JJ denotes same
T9650 7503-7505 IN denotes as
T9651 7506-7508 NN denotes WT
T9652 7509-7510 -LRB- denotes (
T9654 7510-7514 NNS denotes data
T9655 7515-7518 RB denotes not
T9653 7519-7524 VBN denotes shown
T9656 7524-7525 -RRB- denotes )
T9657 7525-7526 . denotes .
T9658 7526-7735 sentence denotes Slightly reduced cell numbers were not due to residual RTC death since we have not observed ectopic apoptosis at any embryonic or postnatal stage in the developing Rb/E2f1 DKO retina (Figures 1D, 1G, and S2).
T9659 7527-7535 RB denotes Slightly
T9660 7536-7543 VBN denotes reduced
T9662 7544-7548 NN denotes cell
T9661 7549-7556 NNS denotes numbers
T9663 7557-7561 VBD denotes were
T9664 7562-7565 RB denotes not
T9665 7566-7569 IN denotes due
T9666 7570-7572 IN denotes to
T9667 7573-7581 JJ denotes residual
T9669 7582-7585 NN denotes RTC
T9668 7586-7591 NN denotes death
T9670 7592-7597 IN denotes since
T9672 7598-7600 PRP denotes we
T9673 7601-7605 VBP denotes have
T9674 7606-7609 RB denotes not
T9671 7610-7618 VBN denotes observed
T9675 7619-7626 JJ denotes ectopic
T9676 7627-7636 NN denotes apoptosis
T9677 7637-7639 IN denotes at
T9678 7640-7643 DT denotes any
T9680 7644-7653 JJ denotes embryonic
T9681 7654-7656 CC denotes or
T9682 7657-7666 JJ denotes postnatal
T9679 7667-7672 NN denotes stage
T9683 7673-7675 IN denotes in
T9684 7676-7679 DT denotes the
T9686 7680-7690 VBG denotes developing
T9687 7691-7693 NN denotes Rb
T9689 7693-7694 HYPH denotes /
T9688 7694-7698 NN denotes E2f1
T9690 7699-7702 NN denotes DKO
T9685 7703-7709 NN denotes retina
T9691 7710-7711 -LRB- denotes (
T9693 7711-7718 NNS denotes Figures
T9692 7719-7721 NN denotes 1D
T9694 7721-7723 , denotes ,
T9695 7723-7725 NN denotes 1G
T9696 7725-7727 , denotes ,
T9697 7727-7730 CC denotes and
T9698 7731-7733 NN denotes S2
T9699 7733-7734 -RRB- denotes )
T9700 7734-7735 . denotes .
T9701 7735-7912 sentence denotes Moreover, deleting Ccnd1, which acts upstream of Rb proteins, also reduces RPC number, but does not suppress any defect in the Rb KO retina (D. C. and R. B., unpublished data).
T9702 7736-7744 RB denotes Moreover
T9704 7744-7746 , denotes ,
T9705 7746-7754 VBG denotes deleting
T9706 7755-7760 NN denotes Ccnd1
T9707 7760-7762 , denotes ,
T9708 7762-7767 WDT denotes which
T9709 7768-7772 VBZ denotes acts
T9710 7773-7781 RB denotes upstream
T9711 7782-7784 IN denotes of
T9712 7785-7787 NN denotes Rb
T9713 7788-7796 NN denotes proteins
T9714 7796-7798 , denotes ,
T9715 7798-7802 RB denotes also
T9703 7803-7810 VBZ denotes reduces
T9716 7811-7814 NN denotes RPC
T9717 7815-7821 NN denotes number
T9718 7821-7823 , denotes ,
T9719 7823-7826 CC denotes but
T9720 7827-7831 VBZ denotes does
T9722 7832-7835 RB denotes not
T9721 7836-7844 VB denotes suppress
T9723 7845-7848 DT denotes any
T9724 7849-7855 NN denotes defect
T9725 7856-7858 IN denotes in
T9726 7859-7862 DT denotes the
T9728 7863-7865 NN denotes Rb
T9729 7866-7868 NN denotes KO
T9727 7869-7875 NN denotes retina
T9730 7876-7877 -LRB- denotes (
T9731 7877-7879 NNP denotes D.
T9732 7880-7882 NNP denotes C.
T9733 7883-7886 CC denotes and
T9734 7887-7889 NNP denotes R.
T9735 7890-7892 NNP denotes B.
T9736 7892-7894 , denotes ,
T9737 7894-7905 JJ denotes unpublished
T9738 7906-7910 NNS denotes data
T9739 7910-7911 -RRB- denotes )
T9740 7911-7912 . denotes .
T9741 7912-8057 sentence denotes Thus, slightly reduced RPC division and dramatic rescue of severe defects in Rb KO RTCs are distinct effects stemming from the deletion of E2f1.
T9742 7913-7917 RB denotes Thus
T9744 7917-7919 , denotes ,
T9745 7919-7927 RB denotes slightly
T9746 7928-7935 JJ denotes reduced
T9748 7936-7939 NN denotes RPC
T9747 7940-7948 NN denotes division
T9749 7949-7952 CC denotes and
T9750 7953-7961 JJ denotes dramatic
T9751 7962-7968 NN denotes rescue
T9752 7969-7971 IN denotes of
T9753 7972-7978 JJ denotes severe
T9754 7979-7986 NNS denotes defects
T9755 7987-7989 IN denotes in
T9756 7990-7992 NN denotes Rb
T9757 7993-7995 NN denotes KO
T9758 7996-8000 NNS denotes RTCs
T9743 8001-8004 VBP denotes are
T9759 8005-8013 JJ denotes distinct
T9760 8014-8021 NNS denotes effects
T9761 8022-8030 VBG denotes stemming
T9762 8031-8035 IN denotes from
T9763 8036-8039 DT denotes the
T9764 8040-8048 NN denotes deletion
T9765 8049-8051 IN denotes of
T9766 8052-8056 NN denotes E2f1
T9767 8056-8057 . denotes .
T11029 8059-8065 JJ denotes Normal
T11030 8066-8074 NN denotes Function
T11031 8075-8077 IN denotes of
T11032 8078-8081 DT denotes the
T11034 8082-8084 NN denotes Rb
T11036 8084-8085 HYPH denotes /
T11035 8085-8089 NN denotes E2f1
T11037 8090-8093 NN denotes DKO
T11033 8094-8100 NN denotes Retina
T11038 8100-8290 sentence denotes The discovery that E2f1 loss rescues even the morphology of Rb KO neurons is surprising because Rb is thought to regulate differentiation primarily through E2f-independent pathways [30–33].
T11039 8101-8104 DT denotes The
T11040 8105-8114 NN denotes discovery
T11042 8115-8119 IN denotes that
T11044 8120-8124 NN denotes E2f1
T11045 8125-8129 NN denotes loss
T11043 8130-8137 VBZ denotes rescues
T11046 8138-8142 RB denotes even
T11048 8143-8146 DT denotes the
T11047 8147-8157 NN denotes morphology
T11049 8158-8160 IN denotes of
T11050 8161-8163 NN denotes Rb
T11051 8164-8166 NN denotes KO
T11052 8167-8174 NNS denotes neurons
T11041 8175-8177 VBZ denotes is
T11053 8178-8188 JJ denotes surprising
T11054 8189-8196 IN denotes because
T11056 8197-8199 NN denotes Rb
T11057 8200-8202 VBZ denotes is
T11055 8203-8210 VBN denotes thought
T11058 8211-8213 TO denotes to
T11059 8214-8222 VB denotes regulate
T11060 8223-8238 NN denotes differentiation
T11061 8239-8248 RB denotes primarily
T11062 8249-8256 IN denotes through
T11063 8257-8260 NN denotes E2f
T11065 8260-8261 HYPH denotes -
T11064 8261-8272 JJ denotes independent
T11066 8273-8281 NNS denotes pathways
T11067 8282-8283 -LRB- denotes [
T11068 8283-8285 CD denotes 30
T11069 8285-8286 SYM denotes –
T11070 8286-8288 CD denotes 33
T11071 8288-8289 -RRB- denotes ]
T11072 8289-8290 . denotes .
T11073 8290-8370 sentence denotes However, normal morphology may not equate to completely normal differentiation.
T11074 8291-8298 RB denotes However
T11076 8298-8300 , denotes ,
T11077 8300-8306 JJ denotes normal
T11078 8307-8317 NN denotes morphology
T11079 8318-8321 MD denotes may
T11080 8322-8325 RB denotes not
T11075 8326-8332 VB denotes equate
T11081 8333-8335 IN denotes to
T11082 8336-8346 RB denotes completely
T11083 8347-8353 JJ denotes normal
T11084 8354-8369 NN denotes differentiation
T11085 8369-8370 . denotes .
T11086 8370-8503 sentence denotes Thus, we compared the electroretinograms (ERGs) of adult WT (α-Cre), E2f1−/−, α-Cre;RbloxP/loxP, and α-Cre;RbloxP/loxP;E2f1−/− mice.
T11087 8371-8375 RB denotes Thus
T11089 8375-8377 , denotes ,
T11090 8377-8379 PRP denotes we
T11088 8380-8388 VBD denotes compared
T11091 8389-8392 DT denotes the
T11092 8393-8411 NNS denotes electroretinograms
T11093 8412-8413 -LRB- denotes (
T11094 8413-8417 NNS denotes ERGs
T11095 8417-8418 -RRB- denotes )
T11096 8419-8421 IN denotes of
T11097 8422-8427 JJ denotes adult
T11099 8428-8430 NN denotes WT
T11100 8431-8432 -LRB- denotes (
T11101 8432-8433 NN denotes α
T11103 8433-8434 HYPH denotes -
T11102 8434-8437 NN denotes Cre
T11104 8437-8438 -RRB- denotes )
T11105 8438-8440 , denotes ,
T11106 8440-8444 NN denotes E2f1
T11107 8444-8445 SYM denotes −
T11108 8445-8446 HYPH denotes /
T11109 8446-8447 SYM denotes −
T11110 8447-8449 , denotes ,
T11111 8449-8450 NN denotes α
T11113 8450-8451 HYPH denotes -
T11112 8451-8454 NN denotes Cre
T11114 8454-8455 : denotes ;
T11115 8455-8461 NN denotes RbloxP
T11117 8461-8462 HYPH denotes /
T11116 8462-8466 NN denotes loxP
T11118 8466-8468 , denotes ,
T11119 8468-8471 CC denotes and
T11120 8472-8473 NN denotes α
T11122 8473-8474 HYPH denotes -
T11121 8474-8477 NN denotes Cre
T11123 8477-8478 : denotes ;
T11124 8478-8484 NN denotes RbloxP
T11126 8484-8485 HYPH denotes /
T11125 8485-8489 NN denotes loxP
T11127 8489-8490 : denotes ;
T11128 8490-8494 NN denotes E2f1
T11129 8494-8495 SYM denotes −
T11130 8495-8496 HYPH denotes /
T11131 8496-8497 SYM denotes −
T11098 8498-8502 NNS denotes mice
T11132 8502-8503 . denotes .
T11133 8503-8690 sentence denotes ERGs functionally assess visual signalling in the mammalian retina from photoreceptors to amacrine cells (but usually not gangion cells), and are dominated by rod and cone bipolar cells.
T11134 8504-8508 NNS denotes ERGs
T11136 8509-8521 RB denotes functionally
T11135 8522-8528 VBP denotes assess
T11137 8529-8535 JJ denotes visual
T11138 8536-8546 NN denotes signalling
T11139 8547-8549 IN denotes in
T11140 8550-8553 DT denotes the
T11142 8554-8563 JJ denotes mammalian
T11141 8564-8570 NN denotes retina
T11143 8571-8575 IN denotes from
T11144 8576-8590 NNS denotes photoreceptors
T11145 8591-8593 IN denotes to
T11146 8594-8602 JJ denotes amacrine
T11147 8603-8608 NNS denotes cells
T11148 8609-8610 -LRB- denotes (
T11149 8610-8613 CC denotes but
T11150 8614-8621 RB denotes usually
T11151 8622-8625 RB denotes not
T11153 8626-8633 NN denotes gangion
T11152 8634-8639 NNS denotes cells
T11154 8639-8640 -RRB- denotes )
T11155 8640-8642 , denotes ,
T11156 8642-8645 CC denotes and
T11157 8646-8649 VBP denotes are
T11158 8650-8659 VBN denotes dominated
T11159 8660-8662 IN denotes by
T11160 8663-8666 NN denotes rod
T11162 8667-8670 CC denotes and
T11163 8671-8675 NN denotes cone
T11164 8676-8683 JJ denotes bipolar
T11161 8684-8689 NNS denotes cells
T11165 8689-8690 . denotes .
T11166 8690-8902 sentence denotes Typically, an ERG signal begins with a negative deflection initiated by the photoreceptors (the a-wave), which is terminated by a large positive deflection due to the activation of ON bipolar cells (the b-wave).
T11167 8691-8700 RB denotes Typically
T11169 8700-8702 , denotes ,
T11170 8702-8704 DT denotes an
T11172 8705-8708 NN denotes ERG
T11171 8709-8715 NN denotes signal
T11168 8716-8722 VBZ denotes begins
T11173 8723-8727 IN denotes with
T11174 8728-8729 DT denotes a
T11176 8730-8738 JJ denotes negative
T11175 8739-8749 NN denotes deflection
T11177 8750-8759 VBN denotes initiated
T11178 8760-8762 IN denotes by
T11179 8763-8766 DT denotes the
T11180 8767-8781 NNS denotes photoreceptors
T11181 8782-8783 -LRB- denotes (
T11182 8783-8786 DT denotes the
T11184 8787-8788 NN denotes a
T11185 8788-8789 HYPH denotes -
T11183 8789-8793 NN denotes wave
T11186 8793-8794 -RRB- denotes )
T11187 8794-8796 , denotes ,
T11188 8796-8801 WDT denotes which
T11190 8802-8804 VBZ denotes is
T11189 8805-8815 VBN denotes terminated
T11191 8816-8818 IN denotes by
T11192 8819-8820 DT denotes a
T11194 8821-8826 JJ denotes large
T11195 8827-8835 JJ denotes positive
T11193 8836-8846 NN denotes deflection
T11196 8847-8850 IN denotes due
T11197 8851-8853 IN denotes to
T11198 8854-8857 DT denotes the
T11199 8858-8868 NN denotes activation
T11200 8869-8871 IN denotes of
T11201 8872-8874 NN denotes ON
T11203 8875-8882 JJ denotes bipolar
T11202 8883-8888 NNS denotes cells
T11204 8889-8890 -LRB- denotes (
T11205 8890-8893 DT denotes the
T11207 8894-8895 NN denotes b
T11208 8895-8896 HYPH denotes -
T11206 8896-8900 NN denotes wave
T11209 8900-8901 -RRB- denotes )
T11210 8901-8902 . denotes .
T11211 8902-9051 sentence denotes Responses to dim light in dark-adapted (scotopic) conditions specifically assess the rod system, and were defective in the Rb KO retina (Figure 2E).
T11212 8903-8912 NNS denotes Responses
T11214 8913-8915 IN denotes to
T11215 8916-8919 JJ denotes dim
T11216 8920-8925 NN denotes light
T11217 8926-8928 IN denotes in
T11218 8929-8933 NN denotes dark
T11220 8933-8934 HYPH denotes -
T11219 8934-8941 VBN denotes adapted
T11222 8942-8943 -LRB- denotes (
T11223 8943-8951 JJ denotes scotopic
T11224 8951-8952 -RRB- denotes )
T11221 8953-8963 NNS denotes conditions
T11225 8964-8976 RB denotes specifically
T11213 8977-8983 VBP denotes assess
T11226 8984-8987 DT denotes the
T11228 8988-8991 NN denotes rod
T11227 8992-8998 NN denotes system
T11229 8998-9000 , denotes ,
T11230 9000-9003 CC denotes and
T11231 9004-9008 VBD denotes were
T11232 9009-9018 JJ denotes defective
T11233 9019-9021 IN denotes in
T11234 9022-9025 DT denotes the
T11236 9026-9028 NN denotes Rb
T11237 9029-9031 NN denotes KO
T11235 9032-9038 NN denotes retina
T11238 9039-9040 -LRB- denotes (
T11240 9040-9046 NN denotes Figure
T11239 9047-9049 NN denotes 2E
T11241 9049-9050 -RRB- denotes )
T11242 9050-9051 . denotes .
T11243 9051-9155 sentence denotes The substantial reduction of both a- and b-waves is consistent with rod and bipolar cell apoptosis [2].
T11244 9052-9055 DT denotes The
T11246 9056-9067 JJ denotes substantial
T11245 9068-9077 NN denotes reduction
T11248 9078-9080 IN denotes of
T11249 9081-9085 CC denotes both
T11250 9086-9087 NN denotes a
T11252 9087-9088 HYPH denotes -
T11253 9089-9092 CC denotes and
T11254 9093-9094 NN denotes b
T11255 9094-9095 HYPH denotes -
T11251 9095-9100 NNS denotes waves
T11247 9101-9103 VBZ denotes is
T11256 9104-9114 JJ denotes consistent
T11257 9115-9119 IN denotes with
T11258 9120-9123 NN denotes rod
T11260 9124-9127 CC denotes and
T11261 9128-9135 JJ denotes bipolar
T11262 9136-9140 NN denotes cell
T11259 9141-9150 NN denotes apoptosis
T11263 9151-9152 -LRB- denotes [
T11264 9152-9153 CD denotes 2
T11265 9153-9154 -RRB- denotes ]
T11266 9154-9155 . denotes .
T11267 9155-9282 sentence denotes The sensitivity of the residual response appeared unchanged, suggesting it arose from the Cre-negative portions of the retina.
T11268 9156-9159 DT denotes The
T11269 9160-9171 NN denotes sensitivity
T11271 9172-9174 IN denotes of
T11272 9175-9178 DT denotes the
T11274 9179-9187 JJ denotes residual
T11273 9188-9196 NN denotes response
T11270 9197-9205 VBD denotes appeared
T11275 9206-9215 JJ denotes unchanged
T11276 9215-9217 , denotes ,
T11277 9217-9227 VBG denotes suggesting
T11278 9228-9230 PRP denotes it
T11279 9231-9236 VBD denotes arose
T11280 9237-9241 IN denotes from
T11281 9242-9245 DT denotes the
T11283 9246-9249 NN denotes Cre
T11285 9249-9250 HYPH denotes -
T11284 9250-9258 JJ denotes negative
T11282 9259-9267 NNS denotes portions
T11286 9268-9270 IN denotes of
T11287 9271-9274 DT denotes the
T11288 9275-9281 NN denotes retina
T11289 9281-9282 . denotes .
T11290 9282-9477 sentence denotes Responses were about the same in the WT and E2f1 KO retina, and, most importantly, also the Rb/E2f1 DKO response median lay at the lower end of the normal range for most intensities (Figure 2F).
T11291 9283-9292 NNS denotes Responses
T11292 9293-9297 VBD denotes were
T11293 9298-9303 RB denotes about
T11295 9304-9307 DT denotes the
T11294 9308-9312 JJ denotes same
T11296 9313-9315 IN denotes in
T11297 9316-9319 DT denotes the
T11299 9320-9322 NN denotes WT
T11300 9323-9326 CC denotes and
T11301 9327-9331 NN denotes E2f1
T11302 9332-9334 NN denotes KO
T11298 9335-9341 NN denotes retina
T11303 9341-9343 , denotes ,
T11304 9343-9346 CC denotes and
T11305 9346-9348 , denotes ,
T11306 9348-9352 RBS denotes most
T11307 9353-9364 RB denotes importantly
T11309 9364-9366 , denotes ,
T11310 9366-9370 RB denotes also
T11311 9371-9374 DT denotes the
T11313 9375-9377 NN denotes Rb
T11315 9377-9378 HYPH denotes /
T11314 9378-9382 NN denotes E2f1
T11316 9383-9386 NN denotes DKO
T11312 9387-9395 NN denotes response
T11317 9396-9402 JJ denotes median
T11308 9403-9406 VBD denotes lay
T11318 9407-9409 IN denotes at
T11319 9410-9413 DT denotes the
T11321 9414-9419 JJ denotes lower
T11320 9420-9423 NN denotes end
T11322 9424-9426 IN denotes of
T11323 9427-9430 DT denotes the
T11325 9431-9437 JJ denotes normal
T11324 9438-9443 NN denotes range
T11326 9444-9447 IN denotes for
T11327 9448-9452 JJS denotes most
T11328 9453-9464 NNS denotes intensities
T11329 9465-9466 -LRB- denotes (
T11331 9466-9472 NN denotes Figure
T11330 9473-9475 NN denotes 2F
T11332 9475-9476 -RRB- denotes )
T11333 9476-9477 . denotes .
T11334 9477-9559 sentence denotes Thus, E2f1 deletion almost completely rescued the rod system in the Rb KO retina.
T11335 9478-9482 RB denotes Thus
T11337 9482-9484 , denotes ,
T11338 9484-9488 NN denotes E2f1
T11339 9489-9497 NN denotes deletion
T11340 9498-9504 RB denotes almost
T11341 9505-9515 RB denotes completely
T11336 9516-9523 VBD denotes rescued
T11342 9524-9527 DT denotes the
T11344 9528-9531 NN denotes rod
T11343 9532-9538 NN denotes system
T11345 9539-9541 IN denotes in
T11346 9542-9545 DT denotes the
T11348 9546-9548 NN denotes Rb
T11349 9549-9551 NN denotes KO
T11347 9552-9558 NN denotes retina
T11350 9558-9559 . denotes .
T11351 9559-9662 sentence denotes Light-adapted (photopic) recordings to specifically assess the cone system yielded comparable results.
T11352 9560-9565 NN denotes Light
T11354 9565-9566 HYPH denotes -
T11353 9566-9573 VBN denotes adapted
T11356 9574-9575 -LRB- denotes (
T11357 9575-9583 JJ denotes photopic
T11358 9583-9584 -RRB- denotes )
T11355 9585-9595 NNS denotes recordings
T11360 9596-9598 TO denotes to
T11362 9599-9611 RB denotes specifically
T11361 9612-9618 VB denotes assess
T11363 9619-9622 DT denotes the
T11365 9623-9627 NN denotes cone
T11364 9628-9634 NN denotes system
T11359 9635-9642 VBD denotes yielded
T11366 9643-9653 JJ denotes comparable
T11367 9654-9661 NNS denotes results
T11368 9661-9662 . denotes .
T11369 9662-9899 sentence denotes Cones represent only 3% of photoreceptors and, unlike rods, develop without Rb, but they require rods for survival, and in the Rb KO retina, they have abnormal morphology and their synaptic targets, bipolar cells, are much depleted [2].
T11370 9663-9668 NNS denotes Cones
T11371 9669-9678 VBP denotes represent
T11372 9679-9683 RB denotes only
T11373 9684-9685 CD denotes 3
T11374 9685-9686 NN denotes %
T11375 9687-9689 IN denotes of
T11376 9690-9704 NNS denotes photoreceptors
T11377 9705-9708 CC denotes and
T11378 9708-9710 , denotes ,
T11379 9710-9716 JJ denotes unlike
T11381 9717-9721 NNS denotes rods
T11382 9721-9723 , denotes ,
T11380 9723-9730 VBP denotes develop
T11383 9731-9738 IN denotes without
T11384 9739-9741 NN denotes Rb
T11385 9741-9743 , denotes ,
T11386 9743-9746 CC denotes but
T11387 9747-9751 PRP denotes they
T11388 9752-9759 VBP denotes require
T11389 9760-9764 NNS denotes rods
T11390 9765-9768 IN denotes for
T11391 9769-9777 NN denotes survival
T11392 9777-9779 , denotes ,
T11393 9779-9782 CC denotes and
T11394 9783-9785 IN denotes in
T11396 9786-9789 DT denotes the
T11398 9790-9792 NN denotes Rb
T11399 9793-9795 NN denotes KO
T11397 9796-9802 NN denotes retina
T11400 9802-9804 , denotes ,
T11401 9804-9808 PRP denotes they
T11395 9809-9813 VBP denotes have
T11402 9814-9822 JJ denotes abnormal
T11403 9823-9833 NN denotes morphology
T11404 9834-9837 CC denotes and
T11405 9838-9843 PRP$ denotes their
T11407 9844-9852 JJ denotes synaptic
T11406 9853-9860 NNS denotes targets
T11409 9860-9862 , denotes ,
T11410 9862-9869 JJ denotes bipolar
T11411 9870-9875 NNS denotes cells
T11412 9875-9877 , denotes ,
T11408 9877-9880 VBP denotes are
T11413 9881-9885 RB denotes much
T11414 9886-9894 JJ denotes depleted
T11415 9895-9896 -LRB- denotes [
T11416 9896-9897 CD denotes 2
T11417 9897-9898 -RRB- denotes ]
T11418 9898-9899 . denotes .
T11419 9899-10066 sentence denotes The photopic response, a product of cone and mainly bipolar activity, was much reduced by Rb loss, but was rescued considerably in the Rb/E2f1 DKO retina (Figure S5).
T11420 9900-9903 DT denotes The
T11422 9904-9912 JJ denotes photopic
T11421 9913-9921 NN denotes response
T11424 9921-9923 , denotes ,
T11425 9923-9924 DT denotes a
T11426 9925-9932 NN denotes product
T11427 9933-9935 IN denotes of
T11428 9936-9940 NN denotes cone
T11430 9941-9944 CC denotes and
T11431 9945-9951 RB denotes mainly
T11432 9952-9959 JJ denotes bipolar
T11429 9960-9968 NN denotes activity
T11433 9968-9970 , denotes ,
T11434 9970-9973 VBD denotes was
T11435 9974-9978 RB denotes much
T11423 9979-9986 VBN denotes reduced
T11436 9987-9989 IN denotes by
T11437 9990-9992 NN denotes Rb
T11438 9993-9997 NN denotes loss
T11439 9997-9999 , denotes ,
T11440 9999-10002 CC denotes but
T11441 10003-10006 VBD denotes was
T11442 10007-10014 VBN denotes rescued
T11443 10015-10027 RB denotes considerably
T11444 10028-10030 IN denotes in
T11445 10031-10034 DT denotes the
T11447 10035-10037 NN denotes Rb
T11449 10037-10038 HYPH denotes /
T11448 10038-10042 NN denotes E2f1
T11450 10043-10046 NN denotes DKO
T11446 10047-10053 NN denotes retina
T11451 10054-10055 -LRB- denotes (
T11453 10055-10061 NN denotes Figure
T11452 10062-10064 NN denotes S5
T11454 10064-10065 -RRB- denotes )
T11455 10065-10066 . denotes .
T11456 10066-10137 sentence denotes Again, the median amplitude lay at the lower end of the E2f1 KO range.
T11457 10067-10072 RB denotes Again
T11459 10072-10074 , denotes ,
T11460 10074-10077 DT denotes the
T11462 10078-10084 JJ denotes median
T11461 10085-10094 NN denotes amplitude
T11458 10095-10098 VBD denotes lay
T11463 10099-10101 IN denotes at
T11464 10102-10105 DT denotes the
T11466 10106-10111 JJ denotes lower
T11465 10112-10115 NN denotes end
T11467 10116-10118 IN denotes of
T11468 10119-10122 DT denotes the
T11470 10123-10127 NN denotes E2f1
T11471 10128-10130 NN denotes KO
T11469 10131-10136 NN denotes range
T11472 10136-10137 . denotes .
T11473 10137-10470 sentence denotes The photopic response in E2f1 KO mice was slightly reduced relative to WT (Figure S5B), possibly because E2f1 is required for maximal expansion of embryonic RPCs, and the E2f1 KO retina has, as noted earlier, slightly fewer cells than the WT retina, although cell type proportions are unaffected (D. C. and R. B., unpublished data).
T11474 10138-10141 DT denotes The
T11476 10142-10150 JJ denotes photopic
T11475 10151-10159 NN denotes response
T11478 10160-10162 IN denotes in
T11479 10163-10167 NN denotes E2f1
T11480 10168-10170 NN denotes KO
T11481 10171-10175 NNS denotes mice
T11482 10176-10179 VBD denotes was
T11483 10180-10188 RB denotes slightly
T11477 10189-10196 VBN denotes reduced
T11484 10197-10205 JJ denotes relative
T11485 10206-10208 IN denotes to
T11486 10209-10211 NN denotes WT
T11487 10212-10213 -LRB- denotes (
T11489 10213-10219 NN denotes Figure
T11488 10220-10223 NN denotes S5B
T11490 10223-10224 -RRB- denotes )
T11491 10224-10226 , denotes ,
T11492 10226-10234 RB denotes possibly
T11494 10235-10242 IN denotes because
T11495 10243-10247 NN denotes E2f1
T11496 10248-10250 VBZ denotes is
T11493 10251-10259 VBN denotes required
T11497 10260-10263 IN denotes for
T11498 10264-10271 JJ denotes maximal
T11499 10272-10281 NN denotes expansion
T11500 10282-10284 IN denotes of
T11501 10285-10294 JJ denotes embryonic
T11502 10295-10299 NNS denotes RPCs
T11503 10299-10301 , denotes ,
T11504 10301-10304 CC denotes and
T11505 10305-10308 DT denotes the
T11507 10309-10313 NN denotes E2f1
T11508 10314-10316 NN denotes KO
T11506 10317-10323 NN denotes retina
T11509 10324-10327 VBZ denotes has
T11510 10327-10329 , denotes ,
T11511 10329-10331 IN denotes as
T11512 10332-10337 VBN denotes noted
T11513 10338-10345 RBR denotes earlier
T11514 10345-10347 , denotes ,
T11515 10347-10355 RB denotes slightly
T11516 10356-10361 JJR denotes fewer
T11517 10362-10367 NNS denotes cells
T11518 10368-10372 IN denotes than
T11519 10373-10376 DT denotes the
T11521 10377-10379 NN denotes WT
T11520 10380-10386 NN denotes retina
T11522 10386-10388 , denotes ,
T11523 10388-10396 IN denotes although
T11525 10397-10401 NN denotes cell
T11526 10402-10406 NN denotes type
T11527 10407-10418 NNS denotes proportions
T11524 10419-10422 VBP denotes are
T11528 10423-10433 JJ denotes unaffected
T11529 10434-10435 -LRB- denotes (
T11530 10435-10437 NNP denotes D.
T11531 10438-10440 NNP denotes C.
T11532 10441-10444 CC denotes and
T11533 10445-10447 NNP denotes R.
T11534 10448-10450 NNP denotes B.
T11535 10450-10452 , denotes ,
T11536 10452-10463 JJ denotes unpublished
T11537 10464-10468 NNS denotes data
T11538 10468-10469 -RRB- denotes )
T11539 10469-10470 . denotes .
T11540 10470-10709 sentence denotes Thus, marginally subnormal photopic responses in the Rb/E2f1 DKO retina can be attributed to both a reduction of cone numbers in E2f1 KO mice alone, and a “genuine” slight reduction in cone function attributable to Rb loss relative to WT.
T11541 10471-10475 RB denotes Thus
T11543 10475-10477 , denotes ,
T11544 10477-10487 RB denotes marginally
T11545 10488-10497 JJ denotes subnormal
T11547 10498-10506 JJ denotes photopic
T11546 10507-10516 NNS denotes responses
T11548 10517-10519 IN denotes in
T11549 10520-10523 DT denotes the
T11551 10524-10526 NN denotes Rb
T11553 10526-10527 HYPH denotes /
T11552 10527-10531 NN denotes E2f1
T11554 10532-10535 NN denotes DKO
T11550 10536-10542 NN denotes retina
T11555 10543-10546 MD denotes can
T11556 10547-10549 VB denotes be
T11542 10550-10560 VBN denotes attributed
T11557 10561-10563 IN denotes to
T11558 10564-10568 CC denotes both
T11560 10569-10570 DT denotes a
T11559 10571-10580 NN denotes reduction
T11561 10581-10583 IN denotes of
T11562 10584-10588 NN denotes cone
T11563 10589-10596 NNS denotes numbers
T11564 10597-10599 IN denotes in
T11565 10600-10604 NN denotes E2f1
T11566 10605-10607 NN denotes KO
T11567 10608-10612 NNS denotes mice
T11568 10613-10618 RB denotes alone
T11569 10618-10620 , denotes ,
T11570 10620-10623 CC denotes and
T11571 10624-10625 DT denotes a
T11573 10626-10627 `` denotes “
T11574 10627-10634 JJ denotes genuine
T11575 10634-10635 '' denotes ”
T11576 10636-10642 JJ denotes slight
T11572 10643-10652 NN denotes reduction
T11577 10653-10655 IN denotes in
T11578 10656-10660 NN denotes cone
T11579 10661-10669 NN denotes function
T11580 10670-10682 JJ denotes attributable
T11581 10683-10685 IN denotes to
T11582 10686-10688 NN denotes Rb
T11583 10689-10693 NN denotes loss
T11584 10694-10702 JJ denotes relative
T11585 10703-10705 IN denotes to
T11586 10706-10708 NN denotes WT
T11587 10708-10709 . denotes .
T11588 10709-10819 sentence denotes This slight effect may relate to a true differentiation defect in a subset of amacrine cells discussed below.
T11589 10710-10714 DT denotes This
T11591 10715-10721 JJ denotes slight
T11590 10722-10728 NN denotes effect
T11593 10729-10732 MD denotes may
T11592 10733-10739 VB denotes relate
T11594 10740-10742 IN denotes to
T11595 10743-10744 DT denotes a
T11597 10745-10749 JJ denotes true
T11598 10750-10765 NN denotes differentiation
T11596 10766-10772 NN denotes defect
T11599 10773-10775 IN denotes in
T11600 10776-10777 DT denotes a
T11601 10778-10784 NN denotes subset
T11602 10785-10787 IN denotes of
T11603 10788-10796 JJ denotes amacrine
T11604 10797-10802 NNS denotes cells
T11605 10803-10812 VBN denotes discussed
T11606 10813-10818 RB denotes below
T11607 10818-10819 . denotes .
T11608 10819-10926 sentence denotes This discussion should not obscure the major outcome that E2f1 deletion recovers most of the ERG response.
T11609 10820-10824 DT denotes This
T11610 10825-10835 NN denotes discussion
T11612 10836-10842 MD denotes should
T11613 10843-10846 RB denotes not
T11611 10847-10854 VB denotes obscure
T11614 10855-10858 DT denotes the
T11616 10859-10864 JJ denotes major
T11615 10865-10872 NN denotes outcome
T11617 10873-10877 IN denotes that
T11619 10878-10882 NN denotes E2f1
T11620 10883-10891 NN denotes deletion
T11618 10892-10900 VBZ denotes recovers
T11621 10901-10905 JJS denotes most
T11622 10906-10908 IN denotes of
T11623 10909-10912 DT denotes the
T11625 10913-10916 NN denotes ERG
T11624 10917-10925 NN denotes response
T11626 10925-10926 . denotes .
T11627 10926-11038 sentence denotes Thus, E2f1 deletion not only rescues morphology but also both rod and cone system function in the Rb KO retina.
T11628 10927-10931 RB denotes Thus
T11630 10931-10933 , denotes ,
T11631 10933-10937 NN denotes E2f1
T11629 10938-10946 NN denotes deletion
T11632 10947-10950 RB denotes not
T11633 10951-10955 RB denotes only
T11634 10956-10963 VBZ denotes rescues
T11635 10964-10974 NN denotes morphology
T11636 10975-10978 CC denotes but
T11637 10979-10983 RB denotes also
T11638 10984-10988 CC denotes both
T11639 10989-10992 NN denotes rod
T11641 10993-10996 CC denotes and
T11642 10997-11001 NN denotes cone
T11643 11002-11008 NN denotes system
T11640 11009-11017 NN denotes function
T11644 11018-11020 IN denotes in
T11645 11021-11024 DT denotes the
T11647 11025-11027 NN denotes Rb
T11648 11028-11030 NN denotes KO
T11646 11031-11037 NN denotes retina
T11649 11037-11038 . denotes .
T13755 11040-11048 JJ denotes Abnormal
T13757 11049-11052 NN denotes SAC
T13756 11053-11068 NN denotes Differentiation
T13758 11069-11080 JJ denotes Independent
T13759 11081-11083 IN denotes of
T13760 11084-11088 NN denotes Cell
T13761 11089-11094 NN denotes Cycle
T13762 11095-11098 CC denotes and
T13763 11099-11107 NN denotes Survival
T13764 11108-11115 NNS denotes Defects
T13765 11115-11231 sentence denotes ERGs primarily assess photoreceptor and bipolar cell function, but may miss differentiation defects in other cells.
T13766 11116-11120 NNS denotes ERGs
T13768 11121-11130 RB denotes primarily
T13767 11131-11137 VBP denotes assess
T13769 11138-11151 NN denotes photoreceptor
T13771 11152-11155 CC denotes and
T13772 11156-11163 JJ denotes bipolar
T13773 11164-11168 NN denotes cell
T13770 11169-11177 NN denotes function
T13774 11177-11179 , denotes ,
T13775 11179-11182 CC denotes but
T13776 11183-11186 MD denotes may
T13777 11187-11191 VB denotes miss
T13778 11192-11207 NN denotes differentiation
T13779 11208-11215 NNS denotes defects
T13780 11216-11218 IN denotes in
T13781 11219-11224 JJ denotes other
T13782 11225-11230 NNS denotes cells
T13783 11230-11231 . denotes .
T13784 11231-11324 sentence denotes To test for subtle differences we stained the Rb/E2f1 DKO retina with 43 markers (Table S1).
T13785 11232-11234 TO denotes To
T13786 11235-11239 VB denotes test
T13788 11240-11243 IN denotes for
T13789 11244-11250 JJ denotes subtle
T13790 11251-11262 NNS denotes differences
T13791 11263-11265 PRP denotes we
T13787 11266-11273 VBD denotes stained
T13792 11274-11277 DT denotes the
T13794 11278-11280 NN denotes Rb
T13796 11280-11281 HYPH denotes /
T13795 11281-11285 NN denotes E2f1
T13797 11286-11289 NN denotes DKO
T13793 11290-11296 NN denotes retina
T13798 11297-11301 IN denotes with
T13799 11302-11304 CD denotes 43
T13800 11305-11312 NNS denotes markers
T13801 11313-11314 -LRB- denotes (
T13803 11314-11319 NN denotes Table
T13802 11320-11322 NN denotes S1
T13804 11322-11323 -RRB- denotes )
T13805 11323-11324 . denotes .
T13806 11324-11424 sentence denotes Thirty-two proteins displayed identical patterns in WT, E2f1 KO, and Rb/E2f1 DKO retina (Table S1).
T13807 11325-11331 CD denotes Thirty
T13809 11331-11332 HYPH denotes -
T13808 11332-11335 CD denotes two
T13810 11336-11344 NN denotes proteins
T13811 11345-11354 VBD denotes displayed
T13812 11355-11364 JJ denotes identical
T13813 11365-11373 NNS denotes patterns
T13814 11374-11376 IN denotes in
T13815 11377-11379 NN denotes WT
T13817 11379-11381 , denotes ,
T13818 11381-11385 NN denotes E2f1
T13819 11386-11388 NN denotes KO
T13820 11388-11390 , denotes ,
T13821 11390-11393 CC denotes and
T13822 11394-11396 NN denotes Rb
T13824 11396-11397 HYPH denotes /
T13823 11397-11401 NN denotes E2f1
T13825 11402-11405 NN denotes DKO
T13816 11406-11412 NN denotes retina
T13826 11413-11414 -LRB- denotes (
T13828 11414-11419 NN denotes Table
T13827 11420-11422 NN denotes S1
T13829 11422-11423 -RRB- denotes )
T13830 11423-11424 . denotes .
T13831 11424-11526 sentence denotes The other 11 markers revealed a cell-cycle– and apoptosis-independent differentiation defect in SACs.
T13832 11425-11428 DT denotes The
T13834 11429-11434 JJ denotes other
T13835 11435-11437 CD denotes 11
T13833 11438-11445 NNS denotes markers
T13836 11446-11454 VBD denotes revealed
T13837 11455-11456 DT denotes a
T13839 11457-11461 NN denotes cell
T13841 11461-11462 HYPH denotes -
T13840 11462-11467 NN denotes cycle
T13843 11467-11468 HYPH denotes –
T13844 11469-11472 CC denotes and
T13845 11473-11482 NN denotes apoptosis
T13846 11482-11483 HYPH denotes -
T13842 11483-11494 JJ denotes independent
T13847 11495-11510 NN denotes differentiation
T13838 11511-11517 NN denotes defect
T13848 11518-11520 IN denotes in
T13849 11521-11525 NNS denotes SACs
T13850 11525-11526 . denotes .
T13851 11526-11702 sentence denotes We first studied Calb2 (calretinin), which marks a subset of amacrine and ganglion cell bodies as well as three tracks corresponding to their processes in the IPL (Figure 3A).
T13852 11527-11529 PRP denotes We
T13854 11530-11535 RB denotes first
T13853 11536-11543 VBD denotes studied
T13855 11544-11549 NN denotes Calb2
T13856 11550-11551 -LRB- denotes (
T13857 11551-11561 NN denotes calretinin
T13858 11561-11562 -RRB- denotes )
T13859 11562-11564 , denotes ,
T13860 11564-11569 WDT denotes which
T13861 11570-11575 VBZ denotes marks
T13862 11576-11577 DT denotes a
T13863 11578-11584 NN denotes subset
T13864 11585-11587 IN denotes of
T13865 11588-11596 JJ denotes amacrine
T13867 11597-11600 CC denotes and
T13868 11601-11609 NN denotes ganglion
T13869 11610-11614 NN denotes cell
T13866 11615-11621 NNS denotes bodies
T13870 11622-11624 RB denotes as
T13872 11625-11629 RB denotes well
T13871 11630-11632 IN denotes as
T13873 11633-11638 CD denotes three
T13874 11639-11645 NNS denotes tracks
T13875 11646-11659 VBG denotes corresponding
T13876 11660-11662 IN denotes to
T13877 11663-11668 PRP$ denotes their
T13878 11669-11678 NNS denotes processes
T13879 11679-11681 IN denotes in
T13880 11682-11685 DT denotes the
T13881 11686-11689 NN denotes IPL
T13882 11690-11691 -LRB- denotes (
T13884 11691-11697 NN denotes Figure
T13883 11698-11700 NN denotes 3A
T13885 11700-11701 -RRB- denotes )
T13886 11701-11702 . denotes .
T13887 11702-11770 sentence denotes Normal Calb2 staining was seen in the E2f1 KO IPL (data not shown).
T13888 11703-11709 JJ denotes Normal
T13890 11710-11715 NN denotes Calb2
T13889 11716-11724 NN denotes staining
T13892 11725-11728 VBD denotes was
T13891 11729-11733 VBN denotes seen
T13893 11734-11736 IN denotes in
T13894 11737-11740 DT denotes the
T13896 11741-11745 NN denotes E2f1
T13897 11746-11748 NN denotes KO
T13895 11749-11752 NN denotes IPL
T13898 11753-11754 -LRB- denotes (
T13900 11754-11758 NNS denotes data
T13901 11759-11762 RB denotes not
T13899 11763-11768 VBN denotes shown
T13902 11768-11769 -RRB- denotes )
T13903 11769-11770 . denotes .
T13904 11770-11902 sentence denotes However, only one Calb2+ track was evident in the Rb KO IPL, and this defect was not rescued in the Rb/E2f1 DKO retina (Figure 3A).
T13905 11771-11778 RB denotes However
T13907 11778-11780 , denotes ,
T13908 11780-11784 RB denotes only
T13910 11785-11788 CD denotes one
T13911 11789-11794 NN denotes Calb2
T13912 11794-11795 SYM denotes +
T13909 11796-11801 NN denotes track
T13906 11802-11805 VBD denotes was
T13913 11806-11813 JJ denotes evident
T13914 11814-11816 IN denotes in
T13915 11817-11820 DT denotes the
T13917 11821-11823 NN denotes Rb
T13918 11824-11826 NN denotes KO
T13916 11827-11830 NN denotes IPL
T13919 11830-11832 , denotes ,
T13920 11832-11835 CC denotes and
T13921 11836-11840 DT denotes this
T13922 11841-11847 NN denotes defect
T13924 11848-11851 VBD denotes was
T13925 11852-11855 RB denotes not
T13923 11856-11863 VBN denotes rescued
T13926 11864-11866 IN denotes in
T13927 11867-11870 DT denotes the
T13929 11871-11873 NN denotes Rb
T13931 11873-11874 HYPH denotes /
T13930 11874-11878 NN denotes E2f1
T13932 11879-11882 NN denotes DKO
T13928 11883-11889 NN denotes retina
T13933 11890-11891 -LRB- denotes (
T13935 11891-11897 NN denotes Figure
T13934 11898-11900 NN denotes 3A
T13936 11900-11901 -RRB- denotes )
T13937 11901-11902 . denotes .
T13938 11902-12061 sentence denotes We quantified Calb2+ cell bodies in the Rb KO INL (corresponding to amacrine cell staining only) and observed a reduction from P8 onwards (Figures 3C and S6).
T13939 11903-11905 PRP denotes We
T13940 11906-11916 VBD denotes quantified
T13941 11917-11922 NN denotes Calb2
T13943 11922-11923 SYM denotes +
T13944 11924-11928 NN denotes cell
T13942 11929-11935 NNS denotes bodies
T13945 11936-11938 IN denotes in
T13946 11939-11942 DT denotes the
T13948 11943-11945 NN denotes Rb
T13949 11946-11948 NN denotes KO
T13947 11949-11952 NN denotes INL
T13950 11953-11954 -LRB- denotes (
T13951 11954-11967 VBG denotes corresponding
T13952 11968-11970 IN denotes to
T13953 11971-11979 JJ denotes amacrine
T13954 11980-11984 NN denotes cell
T13955 11985-11993 NN denotes staining
T13956 11994-11998 RB denotes only
T13957 11998-11999 -RRB- denotes )
T13958 12000-12003 CC denotes and
T13959 12004-12012 VBD denotes observed
T13960 12013-12014 DT denotes a
T13961 12015-12024 NN denotes reduction
T13962 12025-12029 IN denotes from
T13963 12030-12032 NN denotes P8
T13964 12033-12040 RB denotes onwards
T13965 12041-12042 -LRB- denotes (
T13967 12042-12049 NNS denotes Figures
T13966 12050-12052 NN denotes 3C
T13968 12053-12056 CC denotes and
T13969 12057-12059 NN denotes S6
T13970 12059-12060 -RRB- denotes )
T13971 12060-12061 . denotes .
T13972 12061-12062 sentence denotes
T35125 12072-12087 NN denotes Differentiation
T35126 12088-12095 NNS denotes Defects
T35127 12096-12098 IN denotes in
T35128 12099-12101 NN denotes Rb
T35129 12102-12104 NN denotes KO
T35130 12105-12109 NNS denotes SACs
T35131 12109-12246 sentence denotes (A) P18 horizontal sections of WT, Rb KO, and Rb/E2f1 DKO retina were stained for nuclei (DAPI, blue), Calb2 (green), and Slc18a3 (red).
T35132 12110-12111 -LRB- denotes (
T35133 12111-12112 LS denotes A
T35135 12112-12113 -RRB- denotes )
T35136 12114-12117 NN denotes P18
T35138 12118-12128 JJ denotes horizontal
T35137 12129-12137 NNS denotes sections
T35139 12138-12140 IN denotes of
T35140 12141-12143 NN denotes WT
T35142 12143-12145 , denotes ,
T35143 12145-12147 NN denotes Rb
T35144 12148-12150 NN denotes KO
T35145 12150-12152 , denotes ,
T35146 12152-12155 CC denotes and
T35147 12156-12158 NN denotes Rb
T35149 12158-12159 HYPH denotes /
T35148 12159-12163 NN denotes E2f1
T35150 12164-12167 NN denotes DKO
T35141 12168-12174 NN denotes retina
T35151 12175-12179 VBD denotes were
T35134 12180-12187 VBN denotes stained
T35152 12188-12191 IN denotes for
T35153 12192-12198 NNS denotes nuclei
T35154 12199-12200 -LRB- denotes (
T35156 12200-12204 NN denotes DAPI
T35157 12204-12206 , denotes ,
T35155 12206-12210 JJ denotes blue
T35158 12210-12211 -RRB- denotes )
T35159 12211-12213 , denotes ,
T35160 12213-12218 NN denotes Calb2
T35161 12219-12220 -LRB- denotes (
T35162 12220-12225 JJ denotes green
T35163 12225-12226 -RRB- denotes )
T35164 12226-12228 , denotes ,
T35165 12228-12231 CC denotes and
T35166 12232-12239 NN denotes Slc18a3
T35167 12240-12241 -LRB- denotes (
T35168 12241-12244 JJ denotes red
T35169 12244-12245 -RRB- denotes )
T35170 12245-12246 . denotes .
T35171 12246-12403 sentence denotes (B) Confocal images of P30 horizontal sections of WT and Rb KO retina were stained for nuclei (DAPI, blue), Chat and Slc18a3 (both red), and Camk2a (green).
T35172 12247-12248 -LRB- denotes (
T35173 12248-12249 LS denotes B
T35175 12249-12250 -RRB- denotes )
T35176 12251-12259 JJ denotes Confocal
T35177 12260-12266 NNS denotes images
T35178 12267-12269 IN denotes of
T35179 12270-12273 NN denotes P30
T35181 12274-12284 JJ denotes horizontal
T35180 12285-12293 NNS denotes sections
T35182 12294-12296 IN denotes of
T35183 12297-12299 NN denotes WT
T35185 12300-12303 CC denotes and
T35186 12304-12306 NN denotes Rb
T35187 12307-12309 NN denotes KO
T35184 12310-12316 NN denotes retina
T35188 12317-12321 VBD denotes were
T35174 12322-12329 VBN denotes stained
T35189 12330-12333 IN denotes for
T35190 12334-12340 NNS denotes nuclei
T35191 12341-12342 -LRB- denotes (
T35192 12342-12346 NN denotes DAPI
T35193 12346-12348 , denotes ,
T35194 12348-12352 JJ denotes blue
T35195 12352-12353 -RRB- denotes )
T35196 12353-12355 , denotes ,
T35197 12355-12359 NN denotes Chat
T35198 12360-12363 CC denotes and
T35199 12364-12371 NN denotes Slc18a3
T35200 12372-12373 -LRB- denotes (
T35202 12373-12377 RB denotes both
T35201 12378-12381 JJ denotes red
T35203 12381-12382 -RRB- denotes )
T35204 12382-12384 , denotes ,
T35205 12384-12387 CC denotes and
T35206 12388-12394 NN denotes Camk2a
T35207 12395-12396 -LRB- denotes (
T35208 12396-12401 JJ denotes green
T35209 12401-12402 -RRB- denotes )
T35210 12402-12403 . denotes .
T35211 12403-12486 sentence denotes In the Rb KO section, the red stain is Chat only, as Slc18a3 is missing (see [A]).
T35212 12404-12406 IN denotes In
T35214 12407-12410 DT denotes the
T35216 12411-12413 NN denotes Rb
T35217 12414-12416 NN denotes KO
T35215 12417-12424 NN denotes section
T35218 12424-12426 , denotes ,
T35219 12426-12429 DT denotes the
T35221 12430-12433 JJ denotes red
T35220 12434-12439 NN denotes stain
T35213 12440-12442 VBZ denotes is
T35222 12443-12447 NN denotes Chat
T35223 12448-12452 RB denotes only
T35224 12452-12454 , denotes ,
T35225 12454-12456 IN denotes as
T35227 12457-12464 NN denotes Slc18a3
T35228 12465-12467 VBZ denotes is
T35226 12468-12475 VBG denotes missing
T35229 12476-12477 -LRB- denotes (
T35230 12477-12480 VB denotes see
T35231 12481-12482 -LRB- denotes [
T35232 12482-12483 NN denotes A
T35233 12483-12484 -RRB- denotes ]
T35234 12484-12485 -RRB- denotes )
T35235 12485-12486 . denotes .
T35236 12486-12609 sentence denotes (C) Quantification of dense Calb2+ cell bodies in the INL, total Slc18a3+ cell bodies, and Camk2a+ cell bodies in the INL.
T35237 12487-12488 -LRB- denotes (
T35238 12488-12489 LS denotes C
T35240 12489-12490 -RRB- denotes )
T35239 12491-12505 NN denotes Quantification
T35241 12506-12508 IN denotes of
T35242 12509-12514 JJ denotes dense
T35244 12515-12520 NN denotes Calb2
T35245 12520-12521 SYM denotes +
T35246 12522-12526 NN denotes cell
T35243 12527-12533 NNS denotes bodies
T35247 12534-12536 IN denotes in
T35248 12537-12540 DT denotes the
T35249 12541-12544 NN denotes INL
T35250 12544-12546 , denotes ,
T35251 12546-12551 JJ denotes total
T35253 12552-12559 NN denotes Slc18a3
T35254 12559-12560 SYM denotes +
T35255 12561-12565 NN denotes cell
T35252 12566-12572 NNS denotes bodies
T35256 12572-12574 , denotes ,
T35257 12574-12577 CC denotes and
T35258 12578-12584 NN denotes Camk2a
T35260 12584-12585 SYM denotes +
T35261 12586-12590 NN denotes cell
T35259 12591-12597 NNS denotes bodies
T35262 12598-12600 IN denotes in
T35263 12601-12604 DT denotes the
T35264 12605-12608 NN denotes INL
T35265 12608-12609 . denotes .
T35266 12609-12819 sentence denotes Error bars represent SD of measurements from three animals, and asterisks indicate significant differences between retinas of WT and the indicated genotypes (*, p <0.05; **; p <0.01; ANOVA and Tukey HSD test).
T35267 12610-12615 NN denotes Error
T35268 12616-12620 NNS denotes bars
T35269 12621-12630 VBP denotes represent
T35270 12631-12633 NN denotes SD
T35271 12634-12636 IN denotes of
T35272 12637-12649 NNS denotes measurements
T35273 12650-12654 IN denotes from
T35274 12655-12660 CD denotes three
T35275 12661-12668 NNS denotes animals
T35276 12668-12670 , denotes ,
T35277 12670-12673 CC denotes and
T35278 12674-12683 NNS denotes asterisks
T35279 12684-12692 VBP denotes indicate
T35280 12693-12704 JJ denotes significant
T35281 12705-12716 NNS denotes differences
T35282 12717-12724 IN denotes between
T35283 12725-12732 NNS denotes retinas
T35284 12733-12735 IN denotes of
T35285 12736-12738 NN denotes WT
T35286 12739-12742 CC denotes and
T35287 12743-12746 DT denotes the
T35289 12747-12756 VBN denotes indicated
T35288 12757-12766 NNS denotes genotypes
T35290 12767-12768 -LRB- denotes (
T35292 12768-12769 SYM denotes *
T35294 12769-12771 , denotes ,
T35295 12771-12772 NN denotes p
T35296 12773-12774 SYM denotes <
T35293 12774-12778 CD denotes 0.05
T35297 12778-12779 : denotes ;
T35298 12780-12782 SYM denotes **
T35300 12782-12783 : denotes ;
T35301 12784-12785 NN denotes p
T35302 12786-12787 SYM denotes <
T35299 12787-12791 CD denotes 0.01
T35303 12791-12792 : denotes ;
T35304 12793-12798 NN denotes ANOVA
T35305 12799-12802 CC denotes and
T35306 12803-12808 NNP denotes Tukey
T35307 12809-12812 NN denotes HSD
T35291 12813-12817 NN denotes test
T35308 12817-12818 -RRB- denotes )
T35309 12818-12819 . denotes .
T35310 12819-12865 sentence denotes Scale bars are 50 μm in (A) and 20 μm in (B).
T35311 12820-12825 NN denotes Scale
T35312 12826-12830 NNS denotes bars
T35313 12831-12834 VBP denotes are
T35314 12835-12837 CD denotes 50
T35315 12838-12840 NN denotes μm
T35316 12841-12843 IN denotes in
T35317 12844-12845 -LRB- denotes (
T35318 12845-12846 NN denotes A
T35319 12846-12847 -RRB- denotes )
T35320 12848-12851 CC denotes and
T35321 12852-12854 CD denotes 20
T35322 12855-12857 NN denotes μm
T35323 12858-12860 IN denotes in
T35324 12861-12862 -LRB- denotes (
T35325 12862-12863 NN denotes B
T35326 12863-12864 -RRB- denotes )
T35327 12864-12865 . denotes .
T13973 12866-12867 sentence denotes O
T13974 12866-12867 NN denotes O
T13976 12867-12868 IN denotes f
T13975 12867-12870 sentence denotes f t
T13977 12869-12870 NN denotes t
T13980 12870-12871 JJ denotes h
T13978 12870-12878 sentence denotes he three
T13979 12871-12872 NNS denotes e
T13981 12873-12874 NN denotes t
T13982 12874-12875 NN denotes h
T13983 12875-12877 JJ denotes re
T13984 12877-12878 JJ denotes e
T13985 12878-12882 sentence denotes Cal
T13986 12879-12881 NN denotes Ca
T13987 12881-12882 RB denotes l
T13989 12882-12884 NN denotes b2
T13988 12882-12887 sentence denotes b2+ t
T13990 12884-12885 SYM denotes +
T13991 12886-12887 DT denotes t
T13993 12887-12888 NN denotes r
T13992 12887-12892 sentence denotes racks
T13994 12888-12889 NNS denotes a
T13995 12889-12890 VBP denotes c
T13997 12890-12891 NNP denotes k
T13996 12891-12892 NN denotes s
T13998 12892-12896 sentence denotes in
T13999 12893-12895 IN denotes in
T14002 12896-12899 DT denotes the
T14000 12896-13005 sentence denotes the IPL, the two outer tracks are from SACs, named after their extensive dendritic-tree-like morphology [37].
T14003 12900-12903 NN denotes IPL
T14004 12903-12905 , denotes ,
T14005 12905-12908 DT denotes the
T14007 12909-12912 CD denotes two
T14008 12913-12918 JJ denotes outer
T14006 12919-12925 NNS denotes tracks
T14001 12926-12929 VBP denotes are
T14009 12930-12934 IN denotes from
T14010 12935-12939 NNS denotes SACs
T14011 12939-12941 , denotes ,
T14012 12941-12946 VBN denotes named
T14013 12947-12952 IN denotes after
T14014 12953-12958 PRP$ denotes their
T14016 12959-12968 JJ denotes extensive
T14017 12969-12978 JJ denotes dendritic
T14019 12978-12979 HYPH denotes -
T14018 12979-12983 NN denotes tree
T14021 12983-12984 HYPH denotes -
T14020 12984-12988 JJ denotes like
T14015 12989-12999 NN denotes morphology
T14022 13000-13001 -LRB- denotes [
T14023 13001-13003 CD denotes 37
T14024 13003-13004 -RRB- denotes ]
T14025 13004-13005 . denotes .
T14026 13005-13211 sentence denotes SACs are cholinergic, represent ~5.2% of amacrine neurons [38], and are critical for both direction selectivity [34,35] and spontaneous rhythmic activity that occurs during normal retinal development [36].
T14027 13006-13010 NNS denotes SACs
T14028 13011-13014 VBP denotes are
T14029 13015-13026 JJ denotes cholinergic
T14030 13026-13028 , denotes ,
T14031 13028-13037 VBP denotes represent
T14032 13038-13039 SYM denotes ~
T14033 13039-13042 CD denotes 5.2
T14034 13042-13043 NN denotes %
T14035 13044-13046 IN denotes of
T14036 13047-13055 JJ denotes amacrine
T14037 13056-13063 NNS denotes neurons
T14038 13064-13065 -LRB- denotes [
T14039 13065-13067 CD denotes 38
T14040 13067-13068 -RRB- denotes ]
T14041 13068-13070 , denotes ,
T14042 13070-13073 CC denotes and
T14043 13074-13077 VBP denotes are
T14044 13078-13086 JJ denotes critical
T14045 13087-13090 IN denotes for
T14046 13091-13095 CC denotes both
T14048 13096-13105 NN denotes direction
T14047 13106-13117 NN denotes selectivity
T14049 13118-13119 -LRB- denotes [
T14051 13119-13121 CD denotes 34
T14052 13121-13122 , denotes ,
T14050 13122-13124 CD denotes 35
T14053 13124-13125 -RRB- denotes ]
T14054 13126-13129 CC denotes and
T14055 13130-13141 JJ denotes spontaneous
T14057 13142-13150 JJ denotes rhythmic
T14056 13151-13159 NN denotes activity
T14058 13160-13164 WDT denotes that
T14059 13165-13171 VBZ denotes occurs
T14060 13172-13178 IN denotes during
T14061 13179-13185 JJ denotes normal
T14063 13186-13193 JJ denotes retinal
T14062 13194-13205 NN denotes development
T14064 13206-13207 -LRB- denotes [
T14065 13207-13209 CD denotes 36
T14066 13209-13210 -RRB- denotes ]
T14067 13210-13211 . denotes .
T14068 13211-13446 sentence denotes SACs in the INL synapse in the OFF layer of the IPL that responds to decreasing light, while displaced SACs in the GCL have processes that synapse in the nearby ON layer of the IPL that responds to increasing light (reviewed in [39]).
T14069 13212-13216 NNS denotes SACs
T14071 13217-13219 IN denotes in
T14072 13220-13223 DT denotes the
T14073 13224-13227 NN denotes INL
T14070 13228-13235 VBP denotes synapse
T14074 13236-13238 IN denotes in
T14075 13239-13242 DT denotes the
T14077 13243-13246 NN denotes OFF
T14076 13247-13252 NN denotes layer
T14078 13253-13255 IN denotes of
T14079 13256-13259 DT denotes the
T14080 13260-13263 NN denotes IPL
T14081 13264-13268 WDT denotes that
T14082 13269-13277 VBZ denotes responds
T14083 13278-13280 IN denotes to
T14084 13281-13291 VBG denotes decreasing
T14085 13292-13297 NN denotes light
T14086 13297-13299 , denotes ,
T14087 13299-13304 IN denotes while
T14089 13305-13314 VBN denotes displaced
T14090 13315-13319 NNS denotes SACs
T14091 13320-13322 IN denotes in
T14092 13323-13326 DT denotes the
T14093 13327-13330 NN denotes GCL
T14088 13331-13335 VBP denotes have
T14094 13336-13345 NNS denotes processes
T14095 13346-13350 WDT denotes that
T14096 13351-13358 VBP denotes synapse
T14097 13359-13361 IN denotes in
T14098 13362-13365 DT denotes the
T14100 13366-13372 JJ denotes nearby
T14101 13373-13375 NN denotes ON
T14099 13376-13381 NN denotes layer
T14102 13382-13384 IN denotes of
T14103 13385-13388 DT denotes the
T14104 13389-13392 NN denotes IPL
T14105 13393-13397 WDT denotes that
T14106 13398-13406 VBZ denotes responds
T14107 13407-13409 IN denotes to
T14108 13410-13420 VBG denotes increasing
T14109 13421-13426 NN denotes light
T14110 13427-13428 -LRB- denotes (
T14111 13428-13436 VBN denotes reviewed
T14112 13437-13439 IN denotes in
T14113 13440-13441 -LRB- denotes [
T14114 13441-13443 CD denotes 39
T14115 13443-13444 -RRB- denotes ]
T14116 13444-13445 -RRB- denotes )
T14117 13445-13446 . denotes .
T14118 13446-13665 sentence denotes Mature SAC processes stain specifically for Slc18a3 (vesicular acetyl choline transporter, VAChT) [37], and, significantly, this marker was absent in the peripheral Rb KO or Rb/E2f1 DKO P18 retina (Figures 3A and S7B).
T14119 13447-13453 JJ denotes Mature
T14121 13454-13457 NN denotes SAC
T14120 13458-13467 NNS denotes processes
T14122 13468-13473 VBP denotes stain
T14123 13474-13486 RB denotes specifically
T14124 13487-13490 IN denotes for
T14125 13491-13498 NN denotes Slc18a3
T14126 13499-13500 -LRB- denotes (
T14127 13500-13509 JJ denotes vesicular
T14129 13510-13516 NN denotes acetyl
T14130 13517-13524 NN denotes choline
T14128 13525-13536 NN denotes transporter
T14131 13536-13538 , denotes ,
T14132 13538-13543 NN denotes VAChT
T14133 13543-13544 -RRB- denotes )
T14134 13545-13546 -LRB- denotes [
T14135 13546-13548 CD denotes 37
T14136 13548-13549 -RRB- denotes ]
T14137 13549-13551 , denotes ,
T14138 13551-13554 CC denotes and
T14139 13554-13556 , denotes ,
T14140 13556-13569 RB denotes significantly
T14142 13569-13571 , denotes ,
T14143 13571-13575 DT denotes this
T14144 13576-13582 NN denotes marker
T14141 13583-13586 VBD denotes was
T14145 13587-13593 JJ denotes absent
T14146 13594-13596 IN denotes in
T14147 13597-13600 DT denotes the
T14149 13601-13611 JJ denotes peripheral
T14150 13612-13614 NN denotes Rb
T14151 13615-13617 NN denotes KO
T14152 13618-13620 CC denotes or
T14153 13621-13623 NN denotes Rb
T14155 13623-13624 HYPH denotes /
T14154 13624-13628 NN denotes E2f1
T14156 13629-13632 NN denotes DKO
T14157 13633-13636 NN denotes P18
T14148 13637-13643 NN denotes retina
T14158 13644-13645 -LRB- denotes (
T14160 13645-13652 NNS denotes Figures
T14159 13653-13655 NN denotes 3A
T14161 13656-13659 CC denotes and
T14162 13660-13663 NN denotes S7B
T14163 13663-13664 -RRB- denotes )
T14164 13664-13665 . denotes .
T14165 13665-13786 sentence denotes Chat, expressed from the same locus, is also SAC specific, but marks both cell bodies and processes of mature SACs [37].
T14166 13666-13670 NN denotes Chat
T14168 13670-13672 , denotes ,
T14169 13672-13681 VBN denotes expressed
T14170 13682-13686 IN denotes from
T14171 13687-13690 DT denotes the
T14173 13691-13695 JJ denotes same
T14172 13696-13701 NN denotes locus
T14174 13701-13703 , denotes ,
T14167 13703-13705 VBZ denotes is
T14175 13706-13710 RB denotes also
T14176 13711-13714 NN denotes SAC
T14177 13715-13723 JJ denotes specific
T14178 13723-13725 , denotes ,
T14179 13725-13728 CC denotes but
T14180 13729-13734 VBZ denotes marks
T14181 13735-13739 CC denotes both
T14183 13740-13744 NN denotes cell
T14182 13745-13751 NNS denotes bodies
T14184 13752-13755 CC denotes and
T14185 13756-13765 NNS denotes processes
T14186 13766-13768 IN denotes of
T14187 13769-13775 JJ denotes mature
T14188 13776-13780 NNS denotes SACs
T14189 13781-13782 -LRB- denotes [
T14190 13782-13784 CD denotes 37
T14191 13784-13785 -RRB- denotes ]
T14192 13785-13786 . denotes .
T14193 13786-13910 sentence denotes Chat was seen in fewer cells in the mature Rb KO retina, and was present in the soma but absent from processes (Figure 3B).
T14194 13787-13791 NN denotes Chat
T14196 13792-13795 VBD denotes was
T14195 13796-13800 VBN denotes seen
T14197 13801-13803 IN denotes in
T14198 13804-13809 JJR denotes fewer
T14199 13810-13815 NNS denotes cells
T14200 13816-13818 IN denotes in
T14201 13819-13822 DT denotes the
T14203 13823-13829 JJ denotes mature
T14204 13830-13832 NN denotes Rb
T14205 13833-13835 NN denotes KO
T14202 13836-13842 NN denotes retina
T14206 13842-13844 , denotes ,
T14207 13844-13847 CC denotes and
T14208 13848-13851 VBD denotes was
T14209 13852-13859 JJ denotes present
T14210 13860-13862 IN denotes in
T14211 13863-13866 DT denotes the
T14212 13867-13871 NN denotes soma
T14213 13872-13875 CC denotes but
T14214 13876-13882 JJ denotes absent
T14215 13883-13887 IN denotes from
T14216 13888-13897 NNS denotes processes
T14217 13898-13899 -LRB- denotes (
T14219 13899-13905 NN denotes Figure
T14218 13906-13908 NN denotes 3B
T14220 13908-13909 -RRB- denotes )
T14221 13909-13910 . denotes .
T14222 13910-14443 sentence denotes We obtained similar results for Sv2c, a synaptic vesicle protein found in SACs [40]; Kcnc1b and Kcnc2, potassium channels expressed on SAC soma and dendrites as well as a very small number of ganglion cells [41]; gamma-aminobutyric acid (GABA), an inhibitory neurotransmitter present in about half of amacrine cells including SACs, as well as horizontal and some bipolar neurons [37]; and Calb1 (calbindin), which is expressed in many amacrine cells and labels SAC process faintly (Figure S7A and S7B; Table S1; and data not shown).
T14223 13911-13913 PRP denotes We
T14224 13914-13922 VBD denotes obtained
T14225 13923-13930 JJ denotes similar
T14226 13931-13938 NNS denotes results
T14227 13939-13942 IN denotes for
T14228 13943-13947 NN denotes Sv2c
T14229 13947-13949 , denotes ,
T14230 13949-13950 DT denotes a
T14232 13951-13959 JJ denotes synaptic
T14233 13960-13967 NN denotes vesicle
T14231 13968-13975 NN denotes protein
T14234 13976-13981 VBN denotes found
T14235 13982-13984 IN denotes in
T14236 13985-13989 NNS denotes SACs
T14237 13990-13991 -LRB- denotes [
T14238 13991-13993 CD denotes 40
T14239 13993-13994 -RRB- denotes ]
T14240 13994-13995 : denotes ;
T14241 13996-14002 NN denotes Kcnc1b
T14242 14003-14006 CC denotes and
T14243 14007-14012 NN denotes Kcnc2
T14244 14012-14014 , denotes ,
T14245 14014-14023 NN denotes potassium
T14246 14024-14032 NNS denotes channels
T14247 14033-14042 VBN denotes expressed
T14248 14043-14045 IN denotes on
T14249 14046-14049 NN denotes SAC
T14250 14050-14054 NN denotes soma
T14251 14055-14058 CC denotes and
T14252 14059-14068 VBZ denotes dendrites
T14253 14069-14071 RB denotes as
T14255 14072-14076 RB denotes well
T14254 14077-14079 IN denotes as
T14256 14080-14081 DT denotes a
T14258 14082-14086 RB denotes very
T14259 14087-14092 JJ denotes small
T14257 14093-14099 NN denotes number
T14260 14100-14102 IN denotes of
T14261 14103-14111 NN denotes ganglion
T14262 14112-14117 NNS denotes cells
T14263 14118-14119 -LRB- denotes [
T14264 14119-14121 CD denotes 41
T14265 14121-14122 -RRB- denotes ]
T14266 14122-14123 : denotes ;
T14267 14124-14129 NN denotes gamma
T14269 14129-14130 HYPH denotes -
T14270 14130-14142 JJ denotes aminobutyric
T14268 14143-14147 NN denotes acid
T14271 14148-14149 -LRB- denotes (
T14272 14149-14153 NN denotes GABA
T14273 14153-14154 -RRB- denotes )
T14274 14154-14156 , denotes ,
T14275 14156-14158 DT denotes an
T14277 14159-14169 JJ denotes inhibitory
T14276 14170-14186 NN denotes neurotransmitter
T14278 14187-14194 JJ denotes present
T14279 14195-14197 IN denotes in
T14280 14198-14203 IN denotes about
T14281 14204-14208 NN denotes half
T14282 14209-14211 IN denotes of
T14283 14212-14220 JJ denotes amacrine
T14284 14221-14226 NNS denotes cells
T14285 14227-14236 VBG denotes including
T14286 14237-14241 NNS denotes SACs
T14287 14241-14243 , denotes ,
T14288 14243-14245 RB denotes as
T14290 14246-14250 RB denotes well
T14289 14251-14253 IN denotes as
T14291 14254-14264 JJ denotes horizontal
T14292 14265-14268 CC denotes and
T14293 14269-14273 DT denotes some
T14294 14274-14281 JJ denotes bipolar
T14295 14282-14289 NNS denotes neurons
T14296 14290-14291 -LRB- denotes [
T14297 14291-14293 CD denotes 37
T14298 14293-14294 -RRB- denotes ]
T14299 14294-14295 : denotes ;
T14300 14296-14299 CC denotes and
T14301 14300-14305 NN denotes Calb1
T14302 14306-14307 -LRB- denotes (
T14303 14307-14316 NN denotes calbindin
T14304 14316-14317 -RRB- denotes )
T14305 14317-14319 , denotes ,
T14306 14319-14324 WDT denotes which
T14308 14325-14327 VBZ denotes is
T14307 14328-14337 VBN denotes expressed
T14309 14338-14340 IN denotes in
T14310 14341-14345 JJ denotes many
T14312 14346-14354 JJ denotes amacrine
T14311 14355-14360 NNS denotes cells
T14313 14361-14364 CC denotes and
T14314 14365-14371 VBZ denotes labels
T14315 14372-14375 NN denotes SAC
T14316 14376-14383 NN denotes process
T14317 14384-14391 RB denotes faintly
T14318 14392-14393 -LRB- denotes (
T14320 14393-14399 NN denotes Figure
T14319 14400-14403 NN denotes S7A
T14321 14404-14407 CC denotes and
T14322 14408-14411 NN denotes S7B
T14323 14411-14412 : denotes ;
T14324 14413-14418 NN denotes Table
T14325 14419-14421 NN denotes S1
T14326 14421-14422 : denotes ;
T14327 14423-14426 CC denotes and
T14328 14427-14431 NNS denotes data
T14330 14432-14435 RB denotes not
T14329 14436-14441 VBN denotes shown
T14331 14441-14442 -RRB- denotes )
T14332 14442-14443 . denotes .
T14333 14443-14652 sentence denotes Finally, we also examined the effect of Rb deletion on SAC differentiation using a Chx10-Cre transgene that is expressed in a mosaic pattern across the retina, generating patches of Cre-expressing cells [42].
T14334 14444-14451 RB denotes Finally
T14336 14451-14453 , denotes ,
T14337 14453-14455 PRP denotes we
T14338 14456-14460 RB denotes also
T14335 14461-14469 VBD denotes examined
T14339 14470-14473 DT denotes the
T14340 14474-14480 NN denotes effect
T14341 14481-14483 IN denotes of
T14342 14484-14486 NN denotes Rb
T14343 14487-14495 NN denotes deletion
T14344 14496-14498 IN denotes on
T14345 14499-14502 NN denotes SAC
T14346 14503-14518 NN denotes differentiation
T14347 14519-14524 VBG denotes using
T14348 14525-14526 DT denotes a
T14350 14527-14532 NN denotes Chx10
T14352 14532-14533 HYPH denotes -
T14351 14533-14536 NN denotes Cre
T14349 14537-14546 NN denotes transgene
T14353 14547-14551 WDT denotes that
T14355 14552-14554 VBZ denotes is
T14354 14555-14564 VBN denotes expressed
T14356 14565-14567 IN denotes in
T14357 14568-14569 DT denotes a
T14359 14570-14576 JJ denotes mosaic
T14358 14577-14584 NN denotes pattern
T14360 14585-14591 IN denotes across
T14361 14592-14595 DT denotes the
T14362 14596-14602 NN denotes retina
T14363 14602-14604 , denotes ,
T14364 14604-14614 VBG denotes generating
T14365 14615-14622 NNS denotes patches
T14366 14623-14625 IN denotes of
T14367 14626-14629 NN denotes Cre
T14369 14629-14630 HYPH denotes -
T14368 14630-14640 VBG denotes expressing
T14370 14641-14646 NNS denotes cells
T14371 14647-14648 -LRB- denotes [
T14372 14648-14650 CD denotes 42
T14373 14650-14651 -RRB- denotes ]
T14374 14651-14652 . denotes .
T14375 14652-14820 sentence denotes Consistent with the mosaic deletion pattern, we observed markedly reduced Chat/Slc18a3 staining in the IPL of Chx10-Cre;RbloxP/loxP retina compared to WT (Figure S7C).
T14376 14653-14663 JJ denotes Consistent
T14378 14664-14668 IN denotes with
T14379 14669-14672 DT denotes the
T14381 14673-14679 JJ denotes mosaic
T14382 14680-14688 NN denotes deletion
T14380 14689-14696 NN denotes pattern
T14383 14696-14698 , denotes ,
T14384 14698-14700 PRP denotes we
T14377 14701-14709 VBD denotes observed
T14385 14710-14718 RB denotes markedly
T14386 14719-14726 VBN denotes reduced
T14388 14727-14731 NN denotes Chat
T14390 14731-14732 HYPH denotes /
T14389 14732-14739 NN denotes Slc18a3
T14387 14740-14748 NN denotes staining
T14391 14749-14751 IN denotes in
T14392 14752-14755 DT denotes the
T14393 14756-14759 NN denotes IPL
T14394 14760-14762 IN denotes of
T14395 14763-14768 NN denotes Chx10
T14397 14768-14769 HYPH denotes -
T14396 14769-14772 NN denotes Cre
T14399 14772-14773 : denotes ;
T14400 14773-14779 NN denotes RbloxP
T14402 14779-14780 HYPH denotes /
T14401 14780-14784 NN denotes loxP
T14398 14785-14791 NN denotes retina
T14403 14792-14800 VBN denotes compared
T14404 14801-14803 IN denotes to
T14405 14804-14806 NN denotes WT
T14406 14807-14808 -LRB- denotes (
T14408 14808-14814 NN denotes Figure
T14407 14815-14818 NN denotes S7C
T14409 14818-14819 -RRB- denotes )
T14410 14819-14820 . denotes .
T14411 14820-14890 sentence denotes Together, these results suggest a role for Rb in SAC differentiation.
T14412 14821-14829 RB denotes Together
T14414 14829-14831 , denotes ,
T14415 14831-14836 DT denotes these
T14416 14837-14844 NNS denotes results
T14413 14845-14852 VBP denotes suggest
T14417 14853-14854 DT denotes a
T14418 14855-14859 NN denotes role
T14419 14860-14863 IN denotes for
T14420 14864-14866 NN denotes Rb
T14421 14867-14869 IN denotes in
T14422 14870-14873 NN denotes SAC
T14423 14874-14889 NN denotes differentiation
T14424 14889-14890 . denotes .
T14425 14890-15032 sentence denotes The above findings could indicate a defect in SAC specification, SAC survival, or the levels and/or transport of the markers described above.
T14426 14891-14894 DT denotes The
T14428 14895-14900 JJ denotes above
T14427 14901-14909 NNS denotes findings
T14430 14910-14915 MD denotes could
T14429 14916-14924 VB denotes indicate
T14431 14925-14926 DT denotes a
T14432 14927-14933 NN denotes defect
T14433 14934-14936 IN denotes in
T14434 14937-14940 NN denotes SAC
T14435 14941-14954 NN denotes specification
T14436 14954-14956 , denotes ,
T14437 14956-14959 NN denotes SAC
T14438 14960-14968 NN denotes survival
T14439 14968-14970 , denotes ,
T14440 14970-14972 CC denotes or
T14441 14973-14976 DT denotes the
T14442 14977-14983 NNS denotes levels
T14443 14984-14987 CC denotes and
T14444 14987-14988 HYPH denotes /
T14445 14988-14990 CC denotes or
T14446 14991-15000 NN denotes transport
T14447 15001-15003 IN denotes of
T14448 15004-15007 DT denotes the
T14449 15008-15015 NNS denotes markers
T14450 15016-15025 VBN denotes described
T14451 15026-15031 RB denotes above
T14452 15031-15032 . denotes .
T14453 15032-15192 sentence denotes Camk2a marks both SACs and ganglion cells [37], but because ganglion cells are eliminated in the Rb KO retina, Camk2a is a specific SAC marker in this context.
T14454 15033-15039 NN denotes Camk2a
T14455 15040-15045 VBZ denotes marks
T14456 15046-15050 CC denotes both
T14457 15051-15055 NNS denotes SACs
T14458 15056-15059 CC denotes and
T14459 15060-15068 NN denotes ganglion
T14460 15069-15074 NNS denotes cells
T14461 15075-15076 -LRB- denotes [
T14462 15076-15078 CD denotes 37
T14463 15078-15079 -RRB- denotes ]
T14464 15079-15081 , denotes ,
T14465 15081-15084 CC denotes but
T14466 15085-15092 IN denotes because
T14468 15093-15101 NN denotes ganglion
T14469 15102-15107 NNS denotes cells
T14470 15108-15111 VBP denotes are
T14467 15112-15122 VBN denotes eliminated
T14472 15123-15125 IN denotes in
T14473 15126-15129 DT denotes the
T14475 15130-15132 NN denotes Rb
T14476 15133-15135 NN denotes KO
T14474 15136-15142 NN denotes retina
T14477 15142-15144 , denotes ,
T14478 15144-15150 NN denotes Camk2a
T14471 15151-15153 VBZ denotes is
T14479 15154-15155 DT denotes a
T14481 15156-15164 JJ denotes specific
T14482 15165-15168 NN denotes SAC
T14480 15169-15175 NN denotes marker
T14483 15176-15178 IN denotes in
T14484 15179-15183 DT denotes this
T14485 15184-15191 NN denotes context
T14486 15191-15192 . denotes .
T14487 15192-15489 sentence denotes Importantly, Camk2a+ tracks and dendrites were present in both the WT and Rb KO retina (Figure 3B), and the number of Camk2a+ soma was similar in WT and Rb KO retina at P30 and beyond, although fewer cells stained in Rb KO retina at P18, suggesting a delay in its appearance (Figures 3C and S6B).
T14488 15193-15204 RB denotes Importantly
T14490 15204-15206 , denotes ,
T14491 15206-15212 NN denotes Camk2a
T14492 15212-15213 SYM denotes +
T14493 15214-15220 NNS denotes tracks
T14494 15221-15224 CC denotes and
T14495 15225-15234 NNS denotes dendrites
T14489 15235-15239 VBD denotes were
T14496 15240-15247 JJ denotes present
T14497 15248-15250 IN denotes in
T14498 15251-15255 CC denotes both
T14500 15256-15259 DT denotes the
T14501 15260-15262 NN denotes WT
T14502 15263-15266 CC denotes and
T14503 15267-15269 NN denotes Rb
T14504 15270-15272 NN denotes KO
T14499 15273-15279 NN denotes retina
T14505 15280-15281 -LRB- denotes (
T14507 15281-15287 NN denotes Figure
T14506 15288-15290 NN denotes 3B
T14508 15290-15291 -RRB- denotes )
T14509 15291-15293 , denotes ,
T14510 15293-15296 CC denotes and
T14511 15297-15300 DT denotes the
T14512 15301-15307 NN denotes number
T14514 15308-15310 IN denotes of
T14515 15311-15317 NN denotes Camk2a
T14517 15317-15318 SYM denotes +
T14516 15319-15323 NN denotes soma
T14513 15324-15327 VBD denotes was
T14518 15328-15335 JJ denotes similar
T14519 15336-15338 IN denotes in
T14520 15339-15341 NN denotes WT
T14522 15342-15345 CC denotes and
T14523 15346-15348 NN denotes Rb
T14524 15349-15351 NN denotes KO
T14521 15352-15358 NN denotes retina
T14525 15359-15361 IN denotes at
T14526 15362-15365 NN denotes P30
T14527 15366-15369 CC denotes and
T14528 15370-15376 RB denotes beyond
T14529 15376-15378 , denotes ,
T14530 15378-15386 IN denotes although
T14532 15387-15392 JJR denotes fewer
T14533 15393-15398 NNS denotes cells
T14531 15399-15406 VBN denotes stained
T14534 15407-15409 IN denotes in
T14535 15410-15412 NN denotes Rb
T14536 15413-15415 NN denotes KO
T14537 15416-15422 NN denotes retina
T14538 15423-15425 IN denotes at
T14539 15426-15429 NN denotes P18
T14540 15429-15431 , denotes ,
T14541 15431-15441 VBG denotes suggesting
T14542 15442-15443 DT denotes a
T14543 15444-15449 NN denotes delay
T14544 15450-15452 IN denotes in
T14545 15453-15456 PRP$ denotes its
T14546 15457-15467 NN denotes appearance
T14547 15468-15469 -LRB- denotes (
T14549 15469-15476 NNS denotes Figures
T14548 15477-15479 NN denotes 3C
T14550 15480-15483 CC denotes and
T14551 15484-15487 NN denotes S6B
T14552 15487-15488 -RRB- denotes )
T14553 15488-15489 . denotes .
T14554 15489-15743 sentence denotes Thus, Rb is not required for SAC survival or for process outgrowth, but rather it seems to regulate the expression and/or stability of Calb2, Calb1, Chat, Slc18a3, Sv2c, Kcnc1b, Kcnc2, and GABA in SACs, but leaves Camk2a expression virtually unaffected.
T14555 15490-15494 RB denotes Thus
T14557 15494-15496 , denotes ,
T14558 15496-15498 NN denotes Rb
T14559 15499-15501 VBZ denotes is
T14560 15502-15505 RB denotes not
T14556 15506-15514 VBN denotes required
T14561 15515-15518 IN denotes for
T14562 15519-15522 NN denotes SAC
T14563 15523-15531 NN denotes survival
T14564 15532-15534 CC denotes or
T14565 15535-15538 IN denotes for
T14566 15539-15546 NN denotes process
T14567 15547-15556 NN denotes outgrowth
T14568 15556-15558 , denotes ,
T14569 15558-15561 CC denotes but
T14570 15562-15568 RB denotes rather
T14571 15569-15571 PRP denotes it
T14572 15572-15577 VBZ denotes seems
T14573 15578-15580 TO denotes to
T14574 15581-15589 VB denotes regulate
T14575 15590-15593 DT denotes the
T14576 15594-15604 NN denotes expression
T14577 15605-15608 CC denotes and
T14578 15608-15609 HYPH denotes /
T14579 15609-15611 CC denotes or
T14580 15612-15621 NN denotes stability
T14581 15622-15624 IN denotes of
T14582 15625-15630 NN denotes Calb2
T14583 15630-15632 , denotes ,
T14584 15632-15637 NN denotes Calb1
T14585 15637-15639 , denotes ,
T14586 15639-15643 NN denotes Chat
T14587 15643-15645 , denotes ,
T14588 15645-15652 NN denotes Slc18a3
T14589 15652-15654 , denotes ,
T14590 15654-15658 NN denotes Sv2c
T14591 15658-15660 , denotes ,
T14592 15660-15666 NN denotes Kcnc1b
T14593 15666-15668 , denotes ,
T14594 15668-15673 NN denotes Kcnc2
T14595 15673-15675 , denotes ,
T14596 15675-15678 CC denotes and
T14597 15679-15683 NN denotes GABA
T14598 15684-15686 IN denotes in
T14599 15687-15691 NNS denotes SACs
T14600 15691-15693 , denotes ,
T14601 15693-15696 CC denotes but
T14602 15697-15703 VBZ denotes leaves
T14603 15704-15710 NN denotes Camk2a
T14604 15711-15721 NN denotes expression
T14605 15722-15731 RB denotes virtually
T14606 15732-15742 JJ denotes unaffected
T14607 15742-15743 . denotes .
T14608 15743-15853 sentence denotes The presence of Chat in some cell bodies but never in processes (Figure 3B) also suggests a transport defect.
T14609 15744-15747 DT denotes The
T14610 15748-15756 NN denotes presence
T14612 15757-15759 IN denotes of
T14613 15760-15764 NN denotes Chat
T14614 15765-15767 IN denotes in
T14615 15768-15772 DT denotes some
T14617 15773-15777 NN denotes cell
T14616 15778-15784 NNS denotes bodies
T14618 15785-15788 CC denotes but
T14619 15789-15794 RB denotes never
T14620 15795-15797 IN denotes in
T14621 15798-15807 NNS denotes processes
T14622 15808-15809 -LRB- denotes (
T14624 15809-15815 NN denotes Figure
T14623 15816-15818 NN denotes 3B
T14625 15818-15819 -RRB- denotes )
T14626 15820-15824 RB denotes also
T14611 15825-15833 VBZ denotes suggests
T14627 15834-15835 DT denotes a
T14629 15836-15845 NN denotes transport
T14628 15846-15852 NN denotes defect
T14630 15852-15853 . denotes .
T14631 15853-15929 sentence denotes The developmental pattern of Slc18a3 expression also supported this notion.
T14632 15854-15857 DT denotes The
T14634 15858-15871 JJ denotes developmental
T14633 15872-15879 NN denotes pattern
T14636 15880-15882 IN denotes of
T14637 15883-15890 NN denotes Slc18a3
T14638 15891-15901 NN denotes expression
T14639 15902-15906 RB denotes also
T14635 15907-15916 VBD denotes supported
T14640 15917-15921 DT denotes this
T14641 15922-15928 NN denotes notion
T14642 15928-15929 . denotes .
T14643 15929-16094 sentence denotes In mature WT SACs Slc18a3 is only in processes, but in early postnatal SACs, it is found in the cell body, and moves into emerging processes at approximately P4–P6.
T14644 15930-15932 IN denotes In
T14646 15933-15939 JJ denotes mature
T14648 15940-15942 NN denotes WT
T14647 15943-15947 NNS denotes SACs
T14649 15948-15955 NN denotes Slc18a3
T14645 15956-15958 VBZ denotes is
T14650 15959-15963 RB denotes only
T14651 15964-15966 IN denotes in
T14652 15967-15976 NNS denotes processes
T14653 15976-15978 , denotes ,
T14654 15978-15981 CC denotes but
T14655 15982-15984 IN denotes in
T14657 15985-15990 JJ denotes early
T14659 15991-16000 JJ denotes postnatal
T14658 16001-16005 NNS denotes SACs
T14660 16005-16007 , denotes ,
T14661 16007-16009 PRP denotes it
T14662 16010-16012 VBZ denotes is
T14656 16013-16018 VBN denotes found
T14663 16019-16021 IN denotes in
T14664 16022-16025 DT denotes the
T14666 16026-16030 NN denotes cell
T14665 16031-16035 NN denotes body
T14667 16035-16037 , denotes ,
T14668 16037-16040 CC denotes and
T14669 16041-16046 VBZ denotes moves
T14670 16047-16051 IN denotes into
T14671 16052-16060 VBG denotes emerging
T14672 16061-16070 NNS denotes processes
T14673 16071-16073 IN denotes at
T14674 16074-16087 RB denotes approximately
T14675 16088-16090 NN denotes P4
T14676 16090-16091 SYM denotes –
T14677 16091-16093 NN denotes P6
T14678 16093-16094 . denotes .
T14679 16094-16267 sentence denotes As noted above, Slc18a3 was absent at P18 in the Rb KO retina (Figure 3A); at P4 or P5 it was in cell bodies, yet was rarely present in Rb KO processes (Figures 4A and S6).
T14680 16095-16097 IN denotes As
T14681 16098-16103 VBN denotes noted
T14683 16104-16109 RB denotes above
T14684 16109-16111 , denotes ,
T14685 16111-16118 NN denotes Slc18a3
T14682 16119-16122 VBD denotes was
T14687 16123-16129 JJ denotes absent
T14688 16130-16132 IN denotes at
T14689 16133-16136 NN denotes P18
T14690 16137-16139 IN denotes in
T14691 16140-16143 DT denotes the
T14693 16144-16146 NN denotes Rb
T14694 16147-16149 NN denotes KO
T14692 16150-16156 NN denotes retina
T14695 16157-16158 -LRB- denotes (
T14697 16158-16164 NN denotes Figure
T14696 16165-16167 NN denotes 3A
T14698 16167-16168 -RRB- denotes )
T14699 16168-16169 : denotes ;
T14700 16170-16172 IN denotes at
T14701 16173-16175 NN denotes P4
T14702 16176-16178 CC denotes or
T14703 16179-16181 NN denotes P5
T14704 16182-16184 PRP denotes it
T14686 16185-16188 VBD denotes was
T14705 16189-16191 IN denotes in
T14706 16192-16196 NN denotes cell
T14707 16197-16203 NNS denotes bodies
T14708 16203-16205 , denotes ,
T14709 16205-16208 CC denotes yet
T14710 16209-16212 VBD denotes was
T14711 16213-16219 RB denotes rarely
T14712 16220-16227 JJ denotes present
T14713 16228-16230 IN denotes in
T14714 16231-16233 NN denotes Rb
T14715 16234-16236 NN denotes KO
T14716 16237-16246 NNS denotes processes
T14717 16247-16248 -LRB- denotes (
T14719 16248-16255 NNS denotes Figures
T14718 16256-16258 NN denotes 4A
T14720 16259-16262 CC denotes and
T14721 16263-16265 NN denotes S6
T14722 16265-16266 -RRB- denotes )
T14723 16266-16267 . denotes .
T14724 16267-16347 sentence denotes Slc18a3 became virtually undetectable in Rb KO SACs by P8 (Figures 3C and S6C).
T14725 16268-16275 NN denotes Slc18a3
T14726 16276-16282 VBD denotes became
T14727 16283-16292 RB denotes virtually
T14728 16293-16305 JJ denotes undetectable
T14729 16306-16308 IN denotes in
T14730 16309-16311 NN denotes Rb
T14731 16312-16314 NN denotes KO
T14732 16315-16319 NNS denotes SACs
T14733 16320-16322 IN denotes by
T14734 16323-16325 NN denotes P8
T14735 16326-16327 -LRB- denotes (
T14737 16327-16334 NNS denotes Figures
T14736 16335-16337 NN denotes 3C
T14738 16338-16341 CC denotes and
T14739 16342-16345 NN denotes S6C
T14740 16345-16346 -RRB- denotes )
T14741 16346-16347 . denotes .
T14742 16347-16441 sentence denotes These data suggest that Rb affects both the synthesis/stability and transport of SAC markers.
T14743 16348-16353 DT denotes These
T14744 16354-16358 NNS denotes data
T14745 16359-16366 VBP denotes suggest
T14746 16367-16371 IN denotes that
T14748 16372-16374 NN denotes Rb
T14747 16375-16382 VBZ denotes affects
T14749 16383-16387 CC denotes both
T14751 16388-16391 DT denotes the
T14752 16392-16401 NN denotes synthesis
T14753 16401-16402 HYPH denotes /
T14750 16402-16411 NN denotes stability
T14754 16412-16415 CC denotes and
T14755 16416-16425 NN denotes transport
T14756 16426-16428 IN denotes of
T14757 16429-16432 NN denotes SAC
T14758 16433-16440 NNS denotes markers
T14759 16440-16441 . denotes .
T36048 16452-16456 NN denotes E2f3
T36049 16457-16461 NN denotes Loss
T36050 16462-16469 VBZ denotes Rescues
T36051 16470-16485 NN denotes Differentiation
T36052 16486-16488 IN denotes of
T36053 16489-16491 NN denotes Rb
T36054 16492-16494 NN denotes KO
T36055 16495-16499 NNS denotes SACs
T36056 16499-16721 sentence denotes (A) Horizontal retinal sections of the indicated ages and genotypes were stained for nuclei (DAPI, blue), mitosis marker PH3 (green), and Slc18a3 (red), which marks SAC soma at early stages and processes from ~P5 onwards.
T36057 16500-16501 -LRB- denotes (
T36058 16501-16502 LS denotes A
T36060 16502-16503 -RRB- denotes )
T36061 16504-16514 JJ denotes Horizontal
T36063 16515-16522 JJ denotes retinal
T36062 16523-16531 NNS denotes sections
T36064 16532-16534 IN denotes of
T36065 16535-16538 DT denotes the
T36067 16539-16548 VBN denotes indicated
T36066 16549-16553 NNS denotes ages
T36068 16554-16557 CC denotes and
T36069 16558-16567 NNS denotes genotypes
T36070 16568-16572 VBD denotes were
T36059 16573-16580 VBN denotes stained
T36071 16581-16584 IN denotes for
T36072 16585-16591 NNS denotes nuclei
T36073 16592-16593 -LRB- denotes (
T36075 16593-16597 NN denotes DAPI
T36076 16597-16599 , denotes ,
T36074 16599-16603 JJ denotes blue
T36077 16603-16604 -RRB- denotes )
T36078 16604-16606 , denotes ,
T36079 16606-16613 NN denotes mitosis
T36080 16614-16620 NN denotes marker
T36081 16621-16624 NN denotes PH3
T36082 16625-16626 -LRB- denotes (
T36083 16626-16631 JJ denotes green
T36084 16631-16632 -RRB- denotes )
T36085 16632-16634 , denotes ,
T36086 16634-16637 CC denotes and
T36087 16638-16645 NN denotes Slc18a3
T36088 16646-16647 -LRB- denotes (
T36089 16647-16650 JJ denotes red
T36090 16650-16651 -RRB- denotes )
T36091 16651-16653 , denotes ,
T36092 16653-16658 WDT denotes which
T36093 16659-16664 VBZ denotes marks
T36094 16665-16668 NN denotes SAC
T36095 16669-16673 NN denotes soma
T36096 16674-16676 IN denotes at
T36097 16677-16682 JJ denotes early
T36098 16683-16689 NNS denotes stages
T36099 16690-16693 CC denotes and
T36100 16694-16703 NNS denotes processes
T36101 16704-16708 IN denotes from
T36102 16709-16710 SYM denotes ~
T36103 16710-16712 NN denotes P5
T36104 16713-16720 RB denotes onwards
T36105 16720-16721 . denotes .
T36106 16721-16801 sentence denotes Arrows show mitotic PH3+ nuclei in Rb KO, Rb/E2f2 DKO, and Rb/E2f3 DKO retinas.
T36107 16722-16728 NNS denotes Arrows
T36108 16729-16733 VBP denotes show
T36109 16734-16741 JJ denotes mitotic
T36111 16742-16745 NN denotes PH3
T36112 16745-16746 SYM denotes +
T36110 16747-16753 NNS denotes nuclei
T36113 16754-16756 IN denotes in
T36114 16757-16759 NN denotes Rb
T36115 16760-16762 NN denotes KO
T36117 16762-16764 , denotes ,
T36118 16764-16766 NN denotes Rb
T36120 16766-16767 HYPH denotes /
T36119 16767-16771 NN denotes E2f2
T36121 16772-16775 NN denotes DKO
T36122 16775-16777 , denotes ,
T36123 16777-16780 CC denotes and
T36124 16781-16783 NN denotes Rb
T36126 16783-16784 HYPH denotes /
T36125 16784-16788 NN denotes E2f3
T36127 16789-16792 NN denotes DKO
T36116 16793-16800 NNS denotes retinas
T36128 16800-16801 . denotes .
T36129 16801-16887 sentence denotes E2f1 loss rescues the ectopic mitosis and cell death defects, but not the SAC defect.
T36130 16802-16806 NN denotes E2f1
T36131 16807-16811 NN denotes loss
T36132 16812-16819 VBZ denotes rescues
T36133 16820-16823 DT denotes the
T36135 16824-16831 JJ denotes ectopic
T36136 16832-16839 NN denotes mitosis
T36137 16840-16843 CC denotes and
T36138 16844-16848 NN denotes cell
T36139 16849-16854 NN denotes death
T36134 16855-16862 NNS denotes defects
T36140 16862-16864 , denotes ,
T36141 16864-16867 CC denotes but
T36142 16868-16871 RB denotes not
T36143 16872-16875 DT denotes the
T36145 16876-16879 NN denotes SAC
T36144 16880-16886 NN denotes defect
T36146 16886-16887 . denotes .
T36147 16887-16912 sentence denotes E2f2 loss has no effect.
T36148 16888-16892 NN denotes E2f2
T36149 16893-16897 NN denotes loss
T36150 16898-16901 VBZ denotes has
T36151 16902-16904 DT denotes no
T36152 16905-16911 NN denotes effect
T36153 16911-16912 . denotes .
T36154 16912-17009 sentence denotes E2f3 loss does not rescue the ectopic mitosis and cell loss defects, but rescues the SAC defect.
T36155 16913-16917 NN denotes E2f3
T36156 16918-16922 NN denotes loss
T36158 16923-16927 VBZ denotes does
T36159 16928-16931 RB denotes not
T36157 16932-16938 VB denotes rescue
T36160 16939-16942 DT denotes the
T36162 16943-16950 JJ denotes ectopic
T36163 16951-16958 NN denotes mitosis
T36164 16959-16962 CC denotes and
T36165 16963-16967 NN denotes cell
T36166 16968-16972 NN denotes loss
T36161 16973-16980 NNS denotes defects
T36167 16980-16982 , denotes ,
T36168 16982-16985 CC denotes but
T36169 16986-16993 VBZ denotes rescues
T36170 16994-16997 DT denotes the
T36172 16998-17001 NN denotes SAC
T36171 17002-17008 NN denotes defect
T36173 17008-17009 . denotes .
T36174 17009-17103 sentence denotes Inactivating E2f1 and E2f3 together rescues the ectopic mitosis, cell death, and SAC defects.
T36175 17010-17022 VBG denotes Inactivating
T36177 17023-17027 NN denotes E2f1
T36178 17028-17031 CC denotes and
T36179 17032-17036 NN denotes E2f3
T36180 17037-17045 RB denotes together
T36176 17046-17053 VBZ denotes rescues
T36181 17054-17057 DT denotes the
T36183 17058-17065 JJ denotes ectopic
T36184 17066-17073 NN denotes mitosis
T36185 17073-17075 , denotes ,
T36186 17075-17079 NN denotes cell
T36187 17080-17085 NN denotes death
T36188 17085-17087 , denotes ,
T36189 17087-17090 CC denotes and
T36190 17091-17094 NN denotes SAC
T36182 17095-17102 NNS denotes defects
T36191 17102-17103 . denotes .
T36192 17103-17185 sentence denotes (B) The fraction of Camk2a+ cells that are Chat + and Slc18a3+ in the P30 retina.
T36193 17104-17105 -LRB- denotes (
T36194 17105-17106 LS denotes B
T36196 17106-17107 -RRB- denotes )
T36197 17108-17111 DT denotes The
T36195 17112-17120 NN denotes fraction
T36198 17121-17123 IN denotes of
T36199 17124-17130 NN denotes Camk2a
T36201 17130-17131 SYM denotes +
T36200 17132-17137 NNS denotes cells
T36202 17138-17142 WDT denotes that
T36203 17143-17146 VBP denotes are
T36204 17147-17151 NN denotes Chat
T36205 17152-17153 SYM denotes +
T36206 17154-17157 CC denotes and
T36207 17158-17165 NN denotes Slc18a3
T36208 17165-17166 SYM denotes +
T36209 17167-17169 IN denotes in
T36210 17170-17173 DT denotes the
T36212 17174-17177 NN denotes P30
T36211 17178-17184 NN denotes retina
T36213 17184-17185 . denotes .
T36214 17185-17291 sentence denotes (C) Horizontal P5 retinal sections were stained for nuclei (DAPI, blue), Slc18a3 (green), and Isl1 (red).
T36215 17186-17187 -LRB- denotes (
T36216 17187-17188 LS denotes C
T36218 17188-17189 -RRB- denotes )
T36219 17190-17200 JJ denotes Horizontal
T36221 17201-17203 NN denotes P5
T36222 17204-17211 NN denotes retinal
T36220 17212-17220 NNS denotes sections
T36223 17221-17225 VBD denotes were
T36217 17226-17233 VBN denotes stained
T36224 17234-17237 IN denotes for
T36225 17238-17244 NNS denotes nuclei
T36226 17245-17246 -LRB- denotes (
T36228 17246-17250 NN denotes DAPI
T36229 17250-17252 , denotes ,
T36227 17252-17256 JJ denotes blue
T36230 17256-17257 -RRB- denotes )
T36231 17257-17259 , denotes ,
T36232 17259-17266 NN denotes Slc18a3
T36233 17267-17268 -LRB- denotes (
T36234 17268-17273 JJ denotes green
T36235 17273-17274 -RRB- denotes )
T36236 17274-17276 , denotes ,
T36237 17276-17279 CC denotes and
T36238 17280-17284 NN denotes Isl1
T36239 17285-17286 -LRB- denotes (
T36240 17286-17289 JJ denotes red
T36241 17289-17290 -RRB- denotes )
T36242 17290-17291 . denotes .
T36243 17291-17358 sentence denotes Arrows show double-labelled Isl1+/Slc18a3+ cells in the inner INL.
T36244 17292-17298 NNS denotes Arrows
T36245 17299-17303 VBP denotes show
T36246 17304-17310 JJ denotes double
T36248 17310-17311 HYPH denotes -
T36247 17311-17319 VBN denotes labelled
T36250 17320-17324 NN denotes Isl1
T36251 17324-17325 SYM denotes +
T36252 17325-17326 HYPH denotes /
T36253 17326-17333 NN denotes Slc18a3
T36254 17333-17334 SYM denotes +
T36249 17335-17340 NNS denotes cells
T36255 17341-17343 IN denotes in
T36256 17344-17347 DT denotes the
T36258 17348-17353 JJ denotes inner
T36257 17354-17357 NN denotes INL
T36259 17357-17358 . denotes .
T36260 17358-17510 sentence denotes (D) Horizontal P0 retinal sections of the indicated genotypes were stained for nuclei (DAPI, blue), cell division marker Mki67 (green), and Isl1 (red).
T36261 17359-17360 -LRB- denotes (
T36262 17360-17361 LS denotes D
T36264 17361-17362 -RRB- denotes )
T36265 17363-17373 JJ denotes Horizontal
T36267 17374-17376 NN denotes P0
T36268 17377-17384 JJ denotes retinal
T36266 17385-17393 NNS denotes sections
T36269 17394-17396 IN denotes of
T36270 17397-17400 DT denotes the
T36272 17401-17410 VBN denotes indicated
T36271 17411-17420 NNS denotes genotypes
T36273 17421-17425 VBD denotes were
T36263 17426-17433 VBN denotes stained
T36274 17434-17437 IN denotes for
T36275 17438-17444 NNS denotes nuclei
T36276 17445-17446 -LRB- denotes (
T36278 17446-17450 NN denotes DAPI
T36279 17450-17452 , denotes ,
T36277 17452-17456 JJ denotes blue
T36280 17456-17457 -RRB- denotes )
T36281 17457-17459 , denotes ,
T36282 17459-17463 NN denotes cell
T36283 17464-17472 NN denotes division
T36284 17473-17479 NN denotes marker
T36285 17480-17485 NN denotes Mki67
T36286 17486-17487 -LRB- denotes (
T36287 17487-17492 JJ denotes green
T36288 17492-17493 -RRB- denotes )
T36289 17493-17495 , denotes ,
T36290 17495-17498 CC denotes and
T36291 17499-17503 NN denotes Isl1
T36292 17504-17505 -LRB- denotes (
T36293 17505-17508 JJ denotes red
T36294 17508-17509 -RRB- denotes )
T36295 17509-17510 . denotes .
T36296 17510-17553 sentence denotes Arrows show double-labelled dividing SACs.
T36297 17511-17517 NNS denotes Arrows
T36298 17518-17522 VBP denotes show
T36299 17523-17529 JJ denotes double
T36301 17529-17530 HYPH denotes -
T36300 17530-17538 VBN denotes labelled
T36303 17539-17547 VBG denotes dividing
T36302 17548-17552 NNS denotes SACs
T36304 17552-17553 . denotes .
T36305 17553-17651 sentence denotes (E) The fraction of Isl1+ cells in the inner NBL (INBL) of P0 retinas that are dividing (Mki67+).
T36306 17554-17555 -LRB- denotes (
T36307 17555-17556 LS denotes E
T36309 17556-17557 -RRB- denotes )
T36310 17558-17561 DT denotes The
T36308 17562-17570 NN denotes fraction
T36311 17571-17573 IN denotes of
T36312 17574-17578 NN denotes Isl1
T36314 17578-17579 SYM denotes +
T36313 17580-17585 NNS denotes cells
T36315 17586-17588 IN denotes in
T36316 17589-17592 DT denotes the
T36318 17593-17598 JJ denotes inner
T36317 17599-17602 NN denotes NBL
T36319 17603-17604 -LRB- denotes (
T36320 17604-17608 NN denotes INBL
T36321 17608-17609 -RRB- denotes )
T36322 17610-17612 IN denotes of
T36323 17613-17615 NN denotes P0
T36324 17616-17623 NNS denotes retinas
T36325 17624-17628 WDT denotes that
T36327 17629-17632 VBP denotes are
T36326 17633-17641 VBG denotes dividing
T36328 17642-17643 -LRB- denotes (
T36329 17643-17648 NN denotes Mki67
T36330 17648-17649 SYM denotes +
T36331 17649-17650 -RRB- denotes )
T36332 17650-17651 . denotes .
T36333 17651-17861 sentence denotes Error bars represent SD of measurements from three animals, and asterisks indicate significant differences between retinas of WT and the indicated genotypes (*, p <0.05; **; p <0.01; ANOVA and Tukey HSD test).
T36334 17652-17657 NN denotes Error
T36335 17658-17662 NNS denotes bars
T36336 17663-17672 VBP denotes represent
T36337 17673-17675 NN denotes SD
T36338 17676-17678 IN denotes of
T36339 17679-17691 NNS denotes measurements
T36340 17692-17696 IN denotes from
T36341 17697-17702 CD denotes three
T36342 17703-17710 NNS denotes animals
T36343 17710-17712 , denotes ,
T36344 17712-17715 CC denotes and
T36345 17716-17725 NNS denotes asterisks
T36346 17726-17734 VBP denotes indicate
T36347 17735-17746 JJ denotes significant
T36348 17747-17758 NNS denotes differences
T36349 17759-17766 IN denotes between
T36350 17767-17774 NNS denotes retinas
T36351 17775-17777 IN denotes of
T36352 17778-17780 NN denotes WT
T36353 17781-17784 CC denotes and
T36354 17785-17788 DT denotes the
T36355 17789-17798 VBN denotes indicated
T36356 17799-17808 NNS denotes genotypes
T36357 17809-17810 -LRB- denotes (
T36359 17810-17811 SYM denotes *
T36361 17811-17813 , denotes ,
T36362 17813-17814 NN denotes p
T36363 17815-17816 SYM denotes <
T36360 17816-17820 CD denotes 0.05
T36364 17820-17821 : denotes ;
T36365 17822-17824 SYM denotes **
T36367 17824-17825 : denotes ;
T36368 17826-17827 NN denotes p
T36369 17828-17829 SYM denotes <
T36366 17829-17833 CD denotes 0.01
T36370 17833-17834 : denotes ;
T36371 17835-17840 NN denotes ANOVA
T36372 17841-17844 CC denotes and
T36373 17845-17850 NNP denotes Tukey
T36374 17851-17854 NN denotes HSD
T36358 17855-17859 NN denotes test
T36375 17859-17860 -RRB- denotes )
T36376 17860-17861 . denotes .
T36377 17861-17904 sentence denotes Scale bars in (A), (C), and (D) are 50 μm.
T36378 17862-17867 NN denotes Scale
T36379 17868-17872 NNS denotes bars
T36381 17873-17875 IN denotes in
T36382 17876-17877 -LRB- denotes (
T36383 17877-17878 NN denotes A
T36384 17878-17879 -RRB- denotes )
T36385 17879-17881 , denotes ,
T36386 17881-17882 -LRB- denotes (
T36387 17882-17883 NN denotes C
T36388 17883-17884 -RRB- denotes )
T36389 17884-17886 , denotes ,
T36390 17886-17889 CC denotes and
T36391 17890-17891 -LRB- denotes (
T36392 17891-17892 NN denotes D
T36393 17892-17893 -RRB- denotes )
T36380 17894-17897 VBP denotes are
T36394 17898-17900 CD denotes 50
T36395 17901-17903 NN denotes μm
T36396 17903-17904 . denotes .
T16394 17906-17908 NN denotes Rb
T16395 17909-17918 VBZ denotes Regulates
T16396 17919-17922 NN denotes SAC
T16397 17923-17938 NN denotes Differentiation
T16398 17939-17946 IN denotes through
T16399 17947-17951 NN denotes E2f3
T16400 17951-18180 sentence denotes Rb binds more than 100 proteins [43] and in some non-neuronal cells, such as skeletal muscle, adipocytes, and bone, Rb is thought to bind and potentiate tissue-specific transcription factors that promote differentiation [31–33].
T16401 17952-17954 NN denotes Rb
T16402 17955-17960 VBZ denotes binds
T16403 17961-17965 JJR denotes more
T16405 17966-17970 IN denotes than
T16404 17971-17974 CD denotes 100
T16406 17975-17983 NN denotes proteins
T16407 17984-17985 -LRB- denotes [
T16408 17985-17987 CD denotes 43
T16409 17987-17988 -RRB- denotes ]
T16410 17989-17992 CC denotes and
T16411 17993-17995 IN denotes in
T16413 17996-18000 DT denotes some
T16415 18001-18013 JJ denotes non-neuronal
T16414 18014-18019 NNS denotes cells
T16416 18019-18021 , denotes ,
T16417 18021-18025 JJ denotes such
T16418 18026-18028 IN denotes as
T16419 18029-18037 JJ denotes skeletal
T16420 18038-18044 NN denotes muscle
T16421 18044-18046 , denotes ,
T16422 18046-18056 NNS denotes adipocytes
T16423 18056-18058 , denotes ,
T16424 18058-18061 CC denotes and
T16425 18062-18066 NN denotes bone
T16426 18066-18068 , denotes ,
T16427 18068-18070 NN denotes Rb
T16428 18071-18073 VBZ denotes is
T16412 18074-18081 VBN denotes thought
T16429 18082-18084 TO denotes to
T16430 18085-18089 VB denotes bind
T16431 18090-18093 CC denotes and
T16432 18094-18104 VB denotes potentiate
T16433 18105-18111 NN denotes tissue
T16435 18111-18112 HYPH denotes -
T16434 18112-18120 JJ denotes specific
T16437 18121-18134 NN denotes transcription
T16436 18135-18142 NNS denotes factors
T16438 18143-18147 WDT denotes that
T16439 18148-18155 VBP denotes promote
T16440 18156-18171 NN denotes differentiation
T16441 18172-18173 -LRB- denotes [
T16442 18173-18175 CD denotes 31
T16443 18175-18176 SYM denotes –
T16444 18176-18178 CD denotes 33
T16445 18178-18179 -RRB- denotes ]
T16446 18179-18180 . denotes .
T16447 18180-18285 sentence denotes Thus, we expected that Rb might interact with retina-specific factors to facilitate SAC differentiation.
T16448 18181-18185 RB denotes Thus
T16450 18185-18187 , denotes ,
T16451 18187-18189 PRP denotes we
T16449 18190-18198 VBD denotes expected
T16452 18199-18203 IN denotes that
T16454 18204-18206 NN denotes Rb
T16455 18207-18212 MD denotes might
T16453 18213-18221 VB denotes interact
T16456 18222-18226 IN denotes with
T16457 18227-18233 NN denotes retina
T16459 18233-18234 HYPH denotes -
T16458 18234-18242 JJ denotes specific
T16460 18243-18250 NNS denotes factors
T16461 18251-18253 TO denotes to
T16462 18254-18264 VB denotes facilitate
T16463 18265-18268 NN denotes SAC
T16464 18269-18284 NN denotes differentiation
T16465 18284-18285 . denotes .
T16466 18285-18581 sentence denotes A direct role for E2f in mediating Rb-dependent differentiation defects (independent of cell cycle or death defects) has to our knowledge not been described, but because E2f can regulate some differentiation genes [44–48], we first tested whether E2f2 or E2f3 might perturb Rb KO SAC maturation.
T16467 18286-18287 DT denotes A
T16469 18288-18294 JJ denotes direct
T16468 18295-18299 NN denotes role
T16471 18300-18303 IN denotes for
T16472 18304-18307 NN denotes E2f
T16473 18308-18310 IN denotes in
T16474 18311-18320 VBG denotes mediating
T16475 18321-18323 NN denotes Rb
T16477 18323-18324 HYPH denotes -
T16476 18324-18333 JJ denotes dependent
T16479 18334-18349 NN denotes differentiation
T16478 18350-18357 NNS denotes defects
T16480 18358-18359 -LRB- denotes (
T16481 18359-18370 JJ denotes independent
T16482 18371-18373 IN denotes of
T16483 18374-18378 NN denotes cell
T16484 18379-18384 NN denotes cycle
T16486 18385-18387 CC denotes or
T16487 18388-18393 NN denotes death
T16485 18394-18401 NNS denotes defects
T16488 18401-18402 -RRB- denotes )
T16489 18403-18406 VBZ denotes has
T16490 18407-18409 IN denotes to
T16491 18410-18413 PRP$ denotes our
T16492 18414-18423 NN denotes knowledge
T16493 18424-18427 RB denotes not
T16494 18428-18432 VBN denotes been
T16470 18433-18442 VBN denotes described
T16495 18442-18444 , denotes ,
T16496 18444-18447 CC denotes but
T16497 18448-18455 IN denotes because
T16499 18456-18459 NN denotes E2f
T16500 18460-18463 MD denotes can
T16498 18464-18472 VB denotes regulate
T16502 18473-18477 DT denotes some
T16504 18478-18493 NN denotes differentiation
T16503 18494-18499 NNS denotes genes
T16505 18500-18501 -LRB- denotes [
T16506 18501-18503 CD denotes 44
T16507 18503-18504 SYM denotes –
T16508 18504-18506 CD denotes 48
T16509 18506-18507 -RRB- denotes ]
T16510 18507-18509 , denotes ,
T16511 18509-18511 PRP denotes we
T16512 18512-18517 RB denotes first
T16501 18518-18524 VBD denotes tested
T16513 18525-18532 IN denotes whether
T16515 18533-18537 NN denotes E2f2
T16516 18538-18540 CC denotes or
T16517 18541-18545 NN denotes E2f3
T16518 18546-18551 MD denotes might
T16514 18552-18559 VB denotes perturb
T16519 18560-18562 NN denotes Rb
T16520 18563-18565 NN denotes KO
T16521 18566-18569 NN denotes SAC
T16522 18570-18580 NN denotes maturation
T16523 18580-18581 . denotes .
T16524 18581-18759 sentence denotes At multiple time points, E2f1 deletion suppressed ectopic mitosis (PH3+ cells), but did not reverse the SAC defect, and E2f2 deletion had no effect on either defect (Figure 4A).
T16525 18582-18584 IN denotes At
T16527 18585-18593 JJ denotes multiple
T16529 18594-18598 NN denotes time
T16528 18599-18605 NNS denotes points
T16530 18605-18607 , denotes ,
T16531 18607-18611 NN denotes E2f1
T16532 18612-18620 NN denotes deletion
T16526 18621-18631 VBD denotes suppressed
T16533 18632-18639 JJ denotes ectopic
T16534 18640-18647 NN denotes mitosis
T16535 18648-18649 -LRB- denotes (
T16537 18649-18652 NN denotes PH3
T16538 18652-18653 SYM denotes +
T16536 18654-18659 NNS denotes cells
T16539 18659-18660 -RRB- denotes )
T16540 18660-18662 , denotes ,
T16541 18662-18665 CC denotes but
T16542 18666-18669 VBD denotes did
T16544 18670-18673 RB denotes not
T16543 18674-18681 VB denotes reverse
T16545 18682-18685 DT denotes the
T16547 18686-18689 NN denotes SAC
T16546 18690-18696 NN denotes defect
T16548 18696-18698 , denotes ,
T16549 18698-18701 CC denotes and
T16550 18702-18706 NN denotes E2f2
T16551 18707-18715 NN denotes deletion
T16552 18716-18719 VBD denotes had
T16553 18720-18722 DT denotes no
T16554 18723-18729 NN denotes effect
T16555 18730-18732 IN denotes on
T16556 18733-18739 CC denotes either
T16557 18740-18746 NN denotes defect
T16558 18747-18748 -LRB- denotes (
T16560 18748-18754 NN denotes Figure
T16559 18755-18757 NN denotes 4A
T16561 18757-18758 -RRB- denotes )
T16562 18758-18759 . denotes .
T16563 18759-18949 sentence denotes Remarkably, although E2f3 deletion did not reverse ectopic mitosis, it rescued Calb2, Slc18a3, Chat, GABA, Kcnc1b, Kcnc2, and Sv2c staining at multiple times (Figure 4A and data not shown).
T16564 18760-18770 RB denotes Remarkably
T16566 18770-18772 , denotes ,
T16567 18772-18780 IN denotes although
T16569 18781-18785 NN denotes E2f3
T16570 18786-18794 NN denotes deletion
T16571 18795-18798 VBD denotes did
T16572 18799-18802 RB denotes not
T16568 18803-18810 VB denotes reverse
T16573 18811-18818 JJ denotes ectopic
T16574 18819-18826 NN denotes mitosis
T16575 18826-18828 , denotes ,
T16576 18828-18830 PRP denotes it
T16565 18831-18838 VBD denotes rescued
T16577 18839-18844 NN denotes Calb2
T16579 18844-18846 , denotes ,
T16580 18846-18853 NN denotes Slc18a3
T16581 18853-18855 , denotes ,
T16582 18855-18859 NN denotes Chat
T16583 18859-18861 , denotes ,
T16584 18861-18865 NN denotes GABA
T16585 18865-18867 , denotes ,
T16586 18867-18873 NN denotes Kcnc1b
T16587 18873-18875 , denotes ,
T16588 18875-18880 NN denotes Kcnc2
T16589 18880-18882 , denotes ,
T16590 18882-18885 CC denotes and
T16591 18886-18890 NN denotes Sv2c
T16578 18891-18899 NN denotes staining
T16592 18900-18902 IN denotes at
T16593 18903-18911 JJ denotes multiple
T16594 18912-18917 NNS denotes times
T16595 18918-18919 -LRB- denotes (
T16597 18919-18925 NN denotes Figure
T16596 18926-18928 NN denotes 4A
T16598 18929-18932 CC denotes and
T16599 18933-18937 NNS denotes data
T16601 18938-18941 RB denotes not
T16600 18942-18947 VBN denotes shown
T16602 18947-18948 -RRB- denotes )
T16603 18948-18949 . denotes .
T16604 18949-19109 sentence denotes Rb/E2f3 DKO SAC tracks were slightly more disordered than in WT retina, most likely because of the absence of synaptic partner cells, which are killed by E2f1.
T16605 18950-18952 NN denotes Rb
T16607 18952-18953 HYPH denotes /
T16606 18953-18957 NN denotes E2f3
T16608 18958-18961 NN denotes DKO
T16609 18962-18965 NN denotes SAC
T16610 18966-18972 NNS denotes tracks
T16611 18973-18977 VBD denotes were
T16612 18978-18986 RB denotes slightly
T16613 18987-18991 RBR denotes more
T16614 18992-19002 VBN denotes disordered
T16615 19003-19007 IN denotes than
T16616 19008-19010 IN denotes in
T16617 19011-19013 NN denotes WT
T16618 19014-19020 NN denotes retina
T16619 19020-19022 , denotes ,
T16620 19022-19026 RBS denotes most
T16621 19027-19033 RB denotes likely
T16622 19034-19041 IN denotes because
T16623 19042-19044 IN denotes of
T16624 19045-19048 DT denotes the
T16625 19049-19056 NN denotes absence
T16626 19057-19059 IN denotes of
T16627 19060-19068 JJ denotes synaptic
T16629 19069-19076 NN denotes partner
T16628 19077-19082 NNS denotes cells
T16630 19082-19084 , denotes ,
T16631 19084-19089 WDT denotes which
T16633 19090-19093 VBP denotes are
T16632 19094-19100 VBN denotes killed
T16634 19101-19103 IN denotes by
T16635 19104-19108 NN denotes E2f1
T16636 19108-19109 . denotes .
T16637 19109-19291 sentence denotes Indeed, this minor defect was rescued in the Rb/E2f1/E2f3 triple knockout retina, where bipolar and ganglion cell death was rescued and SAC differentiation was restored (Figure 4A).
T16638 19110-19116 RB denotes Indeed
T16640 19116-19118 , denotes ,
T16641 19118-19122 DT denotes this
T16643 19123-19128 JJ denotes minor
T16642 19129-19135 NN denotes defect
T16644 19136-19139 VBD denotes was
T16639 19140-19147 VBN denotes rescued
T16645 19148-19150 IN denotes in
T16646 19151-19154 DT denotes the
T16648 19155-19157 NN denotes Rb
T16650 19157-19158 HYPH denotes /
T16651 19158-19162 NN denotes E2f1
T16652 19162-19163 HYPH denotes /
T16649 19163-19167 NN denotes E2f3
T16654 19168-19174 JJ denotes triple
T16653 19175-19183 NN denotes knockout
T16647 19184-19190 NN denotes retina
T16655 19190-19192 , denotes ,
T16656 19192-19197 WRB denotes where
T16658 19198-19205 JJ denotes bipolar
T16660 19206-19209 CC denotes and
T16661 19210-19218 NN denotes ganglion
T16659 19219-19223 NN denotes cell
T16662 19224-19229 NN denotes death
T16663 19230-19233 VBD denotes was
T16657 19234-19241 VBN denotes rescued
T16664 19242-19245 CC denotes and
T16665 19246-19249 NN denotes SAC
T16666 19250-19265 NN denotes differentiation
T16668 19266-19269 VBD denotes was
T16667 19270-19278 VBN denotes restored
T16669 19279-19280 -LRB- denotes (
T16671 19280-19286 NN denotes Figure
T16670 19287-19289 NN denotes 4A
T16672 19289-19290 -RRB- denotes )
T16673 19290-19291 . denotes .
T16674 19291-19465 sentence denotes E2f3 deletion alone did not affect SAC differentiation (Figure 4A); thus, it is unleashed E2f3 activity that is detrimental, and the critical role for Rb is to inhibit E2f3.
T16675 19292-19296 NN denotes E2f3
T16676 19297-19305 NN denotes deletion
T16678 19306-19311 RB denotes alone
T16679 19312-19315 VBD denotes did
T16680 19316-19319 RB denotes not
T16677 19320-19326 VB denotes affect
T16681 19327-19330 NN denotes SAC
T16682 19331-19346 NN denotes differentiation
T16683 19347-19348 -LRB- denotes (
T16685 19348-19354 NN denotes Figure
T16684 19355-19357 NN denotes 4A
T16686 19357-19358 -RRB- denotes )
T16687 19358-19359 : denotes ;
T16688 19360-19364 RB denotes thus
T16690 19364-19366 , denotes ,
T16691 19366-19368 PRP denotes it
T16689 19369-19371 VBZ denotes is
T16692 19372-19381 VBN denotes unleashed
T16694 19382-19386 NN denotes E2f3
T16693 19387-19395 NN denotes activity
T16695 19396-19400 WDT denotes that
T16696 19401-19403 VBZ denotes is
T16697 19404-19415 JJ denotes detrimental
T16698 19415-19417 , denotes ,
T16699 19417-19420 CC denotes and
T16700 19421-19424 DT denotes the
T16702 19425-19433 JJ denotes critical
T16701 19434-19438 NN denotes role
T16704 19439-19442 IN denotes for
T16705 19443-19445 NN denotes Rb
T16703 19446-19448 VBZ denotes is
T16706 19449-19451 TO denotes to
T16707 19452-19459 VB denotes inhibit
T16708 19460-19464 NN denotes E2f3
T16709 19464-19465 . denotes .
T16710 19465-19757 sentence denotes We quantified the fraction of Camk2a+ SACs in different genotypes and found that 60% of WT P30 Camk2a+ cells expressed Chat and Slc18a3, which dropped to only 5.6% in the Rb KO retina, and remained low at 3.7% in the Rb/E2f1 DKO retina, but rose to 91% in the Rb/E2f3 DKO retina (Figure 4B).
T16711 19466-19468 PRP denotes We
T16712 19469-19479 VBD denotes quantified
T16713 19480-19483 DT denotes the
T16714 19484-19492 NN denotes fraction
T16715 19493-19495 IN denotes of
T16716 19496-19502 NN denotes Camk2a
T16718 19502-19503 SYM denotes +
T16717 19504-19508 NNS denotes SACs
T16719 19509-19511 IN denotes in
T16720 19512-19521 JJ denotes different
T16721 19522-19531 NNS denotes genotypes
T16722 19532-19535 CC denotes and
T16723 19536-19541 VBD denotes found
T16724 19542-19546 IN denotes that
T16726 19547-19549 CD denotes 60
T16727 19549-19550 NN denotes %
T16728 19551-19553 IN denotes of
T16729 19554-19556 NN denotes WT
T16731 19557-19560 NN denotes P30
T16732 19561-19567 NN denotes Camk2a
T16733 19567-19568 SYM denotes +
T16730 19569-19574 NNS denotes cells
T16725 19575-19584 VBD denotes expressed
T16734 19585-19589 NN denotes Chat
T16735 19590-19593 CC denotes and
T16736 19594-19601 NN denotes Slc18a3
T16737 19601-19603 , denotes ,
T16738 19603-19608 WDT denotes which
T16739 19609-19616 VBD denotes dropped
T16740 19617-19619 IN denotes to
T16741 19620-19624 RB denotes only
T16742 19625-19628 CD denotes 5.6
T16743 19628-19629 NN denotes %
T16744 19630-19632 IN denotes in
T16745 19633-19636 DT denotes the
T16747 19637-19639 NN denotes Rb
T16748 19640-19642 NN denotes KO
T16746 19643-19649 NN denotes retina
T16749 19649-19651 , denotes ,
T16750 19651-19654 CC denotes and
T16751 19655-19663 VBD denotes remained
T16752 19664-19667 JJ denotes low
T16753 19668-19670 IN denotes at
T16754 19671-19674 CD denotes 3.7
T16755 19674-19675 NN denotes %
T16756 19676-19678 IN denotes in
T16757 19679-19682 DT denotes the
T16759 19683-19685 NN denotes Rb
T16761 19685-19686 HYPH denotes /
T16760 19686-19690 NN denotes E2f1
T16762 19691-19694 NN denotes DKO
T16758 19695-19701 NN denotes retina
T16763 19701-19703 , denotes ,
T16764 19703-19706 CC denotes but
T16765 19707-19711 VBD denotes rose
T16766 19712-19714 IN denotes to
T16767 19715-19717 CD denotes 91
T16768 19717-19718 NN denotes %
T16769 19719-19721 IN denotes in
T16770 19722-19725 DT denotes the
T16772 19726-19728 NN denotes Rb
T16774 19728-19729 HYPH denotes /
T16773 19729-19733 NN denotes E2f3
T16775 19734-19737 NN denotes DKO
T16771 19738-19744 NN denotes retina
T16776 19745-19746 -LRB- denotes (
T16778 19746-19752 NN denotes Figure
T16777 19753-19755 NN denotes 4B
T16779 19755-19756 -RRB- denotes )
T16780 19756-19757 . denotes .
T16781 19757-19890 sentence denotes The latter fraction is higher than WT because ganglion cells, which normally make up ~40% of Camk2a+ cells, are killed by apoptosis.
T16782 19758-19761 DT denotes The
T16784 19762-19768 JJ denotes latter
T16783 19769-19777 NN denotes fraction
T16785 19778-19780 VBZ denotes is
T16786 19781-19787 JJR denotes higher
T16787 19788-19792 IN denotes than
T16788 19793-19795 NN denotes WT
T16789 19796-19803 IN denotes because
T16791 19804-19812 NN denotes ganglion
T16792 19813-19818 NNS denotes cells
T16793 19818-19820 , denotes ,
T16794 19820-19825 WDT denotes which
T16796 19826-19834 RB denotes normally
T16795 19835-19839 VBP denotes make
T16797 19840-19842 RP denotes up
T16798 19843-19844 SYM denotes ~
T16799 19844-19846 CD denotes 40
T16800 19846-19847 NN denotes %
T16801 19848-19850 IN denotes of
T16802 19851-19857 NN denotes Camk2a
T16804 19857-19858 SYM denotes +
T16803 19859-19864 NNS denotes cells
T16805 19864-19866 , denotes ,
T16806 19866-19869 VBP denotes are
T16790 19870-19876 VBN denotes killed
T16807 19877-19879 IN denotes by
T16808 19880-19889 NN denotes apoptosis
T16809 19889-19890 . denotes .
T16810 19890-20001 sentence denotes To quantify the effect of different E2fs on ectopic division specifically in SACs, we exploited Isl1 (Islet1).
T16811 19891-19893 TO denotes To
T16812 19894-19902 VB denotes quantify
T16814 19903-19906 DT denotes the
T16815 19907-19913 NN denotes effect
T16816 19914-19916 IN denotes of
T16817 19917-19926 JJ denotes different
T16818 19927-19931 NNS denotes E2fs
T16819 19932-19934 IN denotes on
T16820 19935-19942 JJ denotes ectopic
T16821 19943-19951 NN denotes division
T16822 19952-19964 RB denotes specifically
T16823 19965-19967 IN denotes in
T16824 19968-19972 NNS denotes SACs
T16825 19972-19974 , denotes ,
T16826 19974-19976 PRP denotes we
T16813 19977-19986 VBD denotes exploited
T16827 19987-19991 NN denotes Isl1
T16828 19992-19993 -LRB- denotes (
T16829 19993-19999 NN denotes Islet1
T16830 19999-20000 -RRB- denotes )
T16831 20000-20001 . denotes .
T16832 20001-20114 sentence denotes This marker is expressed in both SACs and ganglion cells, thus Isl1+ cells in the INL are exclusively SACs [49].
T16833 20002-20006 DT denotes This
T16834 20007-20013 NN denotes marker
T16836 20014-20016 VBZ denotes is
T16835 20017-20026 VBN denotes expressed
T16838 20027-20029 IN denotes in
T16839 20030-20034 CC denotes both
T16840 20035-20039 NNS denotes SACs
T16841 20040-20043 CC denotes and
T16842 20044-20052 NN denotes ganglion
T16843 20053-20058 NNS denotes cells
T16844 20058-20060 , denotes ,
T16845 20060-20064 RB denotes thus
T16846 20065-20069 NN denotes Isl1
T16848 20069-20070 SYM denotes +
T16847 20071-20076 NNS denotes cells
T16849 20077-20079 IN denotes in
T16850 20080-20083 DT denotes the
T16851 20084-20087 NN denotes INL
T16837 20088-20091 VBP denotes are
T16852 20092-20103 RB denotes exclusively
T16853 20104-20108 NNS denotes SACs
T16854 20109-20110 -LRB- denotes [
T16855 20110-20112 CD denotes 49
T16856 20112-20113 -RRB- denotes ]
T16857 20113-20114 . denotes .
T16858 20114-20268 sentence denotes We found that 98.2% ± 1.8% of Isl1+ cells in the forming inner INL at P5 were also Slc18a3+, confirming that Isl1 is an excellent SAC marker (Figure 4C).
T16859 20115-20117 PRP denotes We
T16860 20118-20123 VBD denotes found
T16861 20124-20128 IN denotes that
T16863 20129-20133 CD denotes 98.2
T16864 20133-20134 NN denotes %
T16865 20135-20136 SYM denotes ±
T16866 20137-20140 CD denotes 1.8
T16867 20140-20141 NN denotes %
T16868 20142-20144 IN denotes of
T16869 20145-20149 NN denotes Isl1
T16871 20149-20150 SYM denotes +
T16870 20151-20156 NNS denotes cells
T16872 20157-20159 IN denotes in
T16873 20160-20163 DT denotes the
T16875 20164-20171 VBG denotes forming
T16876 20172-20177 JJ denotes inner
T16874 20178-20181 NN denotes INL
T16877 20182-20184 IN denotes at
T16878 20185-20187 NN denotes P5
T16862 20188-20192 VBD denotes were
T16879 20193-20197 RB denotes also
T16880 20198-20205 NN denotes Slc18a3
T16881 20205-20206 SYM denotes +
T16882 20206-20208 , denotes ,
T16883 20208-20218 VBG denotes confirming
T16884 20219-20223 IN denotes that
T16886 20224-20228 NN denotes Isl1
T16885 20229-20231 VBZ denotes is
T16887 20232-20234 DT denotes an
T16889 20235-20244 JJ denotes excellent
T16890 20245-20248 NN denotes SAC
T16888 20249-20255 NN denotes marker
T16891 20256-20257 -LRB- denotes (
T16893 20257-20263 NN denotes Figure
T16892 20264-20266 NN denotes 4C
T16894 20266-20267 -RRB- denotes )
T16895 20267-20268 . denotes .
T16896 20268-20361 sentence denotes Moreover, Isl1, unlike Slc18a3, is nuclear, which facilitates scoring of Isl1+/Mki67+ cells.
T16897 20269-20277 RB denotes Moreover
T16899 20277-20279 , denotes ,
T16900 20279-20283 NN denotes Isl1
T16901 20283-20285 , denotes ,
T16902 20285-20291 IN denotes unlike
T16903 20292-20299 NN denotes Slc18a3
T16904 20299-20301 , denotes ,
T16898 20301-20303 VBZ denotes is
T16905 20304-20311 JJ denotes nuclear
T16906 20311-20313 , denotes ,
T16907 20313-20318 WDT denotes which
T16908 20319-20330 VBZ denotes facilitates
T16909 20331-20338 NN denotes scoring
T16910 20339-20341 IN denotes of
T16911 20342-20346 NN denotes Isl1
T16913 20346-20347 SYM denotes +
T16914 20347-20348 HYPH denotes /
T16915 20348-20353 NN denotes Mki67
T16916 20353-20354 SYM denotes +
T16912 20355-20360 NNS denotes cells
T16917 20360-20361 . denotes .
T16918 20361-20646 sentence denotes It is also expressed earlier than Slc18a3, permitting analysis of SACs soon after their birth at ~E15; thus, we could study retina at P0, a time when ectopic division is high in the inner retina and prior to Rb-independent cell cycle exit associated with terminal differentiation [2].
T16919 20362-20364 PRP denotes It
T16921 20365-20367 VBZ denotes is
T16922 20368-20372 RB denotes also
T16920 20373-20382 VBN denotes expressed
T16924 20383-20390 RBR denotes earlier
T16925 20391-20395 IN denotes than
T16926 20396-20403 NN denotes Slc18a3
T16927 20403-20405 , denotes ,
T16928 20405-20415 VBG denotes permitting
T16929 20416-20424 NN denotes analysis
T16930 20425-20427 IN denotes of
T16931 20428-20432 NNS denotes SACs
T16932 20433-20437 RB denotes soon
T16933 20438-20443 IN denotes after
T16934 20444-20449 PRP$ denotes their
T16935 20450-20455 NN denotes birth
T16936 20456-20458 IN denotes at
T16937 20459-20460 SYM denotes ~
T16938 20460-20463 NN denotes E15
T16939 20463-20464 : denotes ;
T16940 20465-20469 RB denotes thus
T16941 20469-20471 , denotes ,
T16942 20471-20473 PRP denotes we
T16943 20474-20479 MD denotes could
T16923 20480-20485 VB denotes study
T16944 20486-20492 NN denotes retina
T16945 20493-20495 IN denotes at
T16946 20496-20498 NN denotes P0
T16947 20498-20500 , denotes ,
T16948 20500-20501 DT denotes a
T16949 20502-20506 NN denotes time
T16950 20507-20511 WRB denotes when
T16952 20512-20519 JJ denotes ectopic
T16953 20520-20528 NN denotes division
T16951 20529-20531 VBZ denotes is
T16954 20532-20536 JJ denotes high
T16955 20537-20539 IN denotes in
T16956 20540-20543 DT denotes the
T16958 20544-20549 JJ denotes inner
T16957 20550-20556 NN denotes retina
T16959 20557-20560 CC denotes and
T16960 20561-20566 RB denotes prior
T16961 20567-20569 IN denotes to
T16962 20570-20572 NN denotes Rb
T16964 20572-20573 HYPH denotes -
T16963 20573-20584 JJ denotes independent
T16966 20585-20589 NN denotes cell
T16967 20590-20595 NN denotes cycle
T16965 20596-20600 NN denotes exit
T16968 20601-20611 VBN denotes associated
T16969 20612-20616 IN denotes with
T16970 20617-20625 JJ denotes terminal
T16971 20626-20641 NN denotes differentiation
T16972 20642-20643 -LRB- denotes [
T16973 20643-20644 CD denotes 2
T16974 20644-20645 -RRB- denotes ]
T16975 20645-20646 . denotes .
T16976 20646-20831 sentence denotes At P0, no WT Isl1+ cells in the inner neuroblastic layer (NBL) (which is the future INL) were dividing, but 57 ± 14 Isl1+/Mki67+ cells were detected in the Rb KO inner NBL (Figure 4D).
T16977 20647-20649 IN denotes At
T16979 20650-20652 NN denotes P0
T16980 20652-20654 , denotes ,
T16981 20654-20656 DT denotes no
T16983 20657-20659 NN denotes WT
T16984 20660-20664 NN denotes Isl1
T16985 20664-20665 SYM denotes +
T16982 20666-20671 NNS denotes cells
T16986 20672-20674 IN denotes in
T16987 20675-20678 DT denotes the
T16989 20679-20684 JJ denotes inner
T16990 20685-20697 JJ denotes neuroblastic
T16988 20698-20703 NN denotes layer
T16991 20704-20705 -LRB- denotes (
T16992 20705-20708 NN denotes NBL
T16993 20708-20709 -RRB- denotes )
T16994 20710-20711 -LRB- denotes (
T16995 20711-20716 WDT denotes which
T16996 20717-20719 VBZ denotes is
T16997 20720-20723 DT denotes the
T16999 20724-20730 JJ denotes future
T16998 20731-20734 NN denotes INL
T17000 20734-20735 -RRB- denotes )
T17001 20736-20740 VBD denotes were
T16978 20741-20749 VBG denotes dividing
T17002 20749-20751 , denotes ,
T17003 20751-20754 CC denotes but
T17004 20755-20757 CD denotes 57
T17006 20758-20759 SYM denotes ±
T17005 20760-20762 CD denotes 14
T17008 20763-20767 NN denotes Isl1
T17009 20767-20768 SYM denotes +
T17010 20768-20769 HYPH denotes /
T17011 20769-20774 NN denotes Mki67
T17012 20774-20775 SYM denotes +
T17007 20776-20781 NNS denotes cells
T17014 20782-20786 VBD denotes were
T17013 20787-20795 VBN denotes detected
T17015 20796-20798 IN denotes in
T17016 20799-20802 DT denotes the
T17018 20803-20805 NN denotes Rb
T17019 20806-20808 NN denotes KO
T17020 20809-20814 JJ denotes inner
T17017 20815-20818 NN denotes NBL
T17021 20819-20820 -LRB- denotes (
T17023 20820-20826 NN denotes Figure
T17022 20827-20829 NN denotes 4D
T17024 20829-20830 -RRB- denotes )
T17025 20830-20831 . denotes .
T17026 20831-21013 sentence denotes Indeed, about one-third of all Isl1+ cells in the entire inner NBL were dividing in the Rb KO retina, or ~50% in the periphery where Cre is expressed (Figure 4E and data not shown).
T17027 20832-20838 RB denotes Indeed
T17029 20838-20840 , denotes ,
T17030 20840-20845 IN denotes about
T17031 20846-20849 CD denotes one
T17032 20849-20850 HYPH denotes -
T17033 20850-20855 NN denotes third
T17034 20856-20858 IN denotes of
T17035 20859-20862 DT denotes all
T17037 20863-20867 NN denotes Isl1
T17038 20867-20868 SYM denotes +
T17036 20869-20874 NNS denotes cells
T17039 20875-20877 IN denotes in
T17040 20878-20881 DT denotes the
T17042 20882-20888 JJ denotes entire
T17043 20889-20894 JJ denotes inner
T17041 20895-20898 NN denotes NBL
T17044 20899-20903 VBD denotes were
T17028 20904-20912 VBG denotes dividing
T17045 20913-20915 IN denotes in
T17046 20916-20919 DT denotes the
T17048 20920-20922 NN denotes Rb
T17049 20923-20925 NN denotes KO
T17047 20926-20932 NN denotes retina
T17050 20932-20934 , denotes ,
T17051 20934-20936 CC denotes or
T17052 20937-20938 SYM denotes ~
T17053 20938-20940 CD denotes 50
T17054 20940-20941 NN denotes %
T17055 20942-20944 IN denotes in
T17056 20945-20948 DT denotes the
T17057 20949-20958 NN denotes periphery
T17058 20959-20964 WRB denotes where
T17060 20965-20968 NN denotes Cre
T17061 20969-20971 VBZ denotes is
T17059 20972-20981 VBN denotes expressed
T17062 20982-20983 -LRB- denotes (
T17064 20983-20989 NN denotes Figure
T17063 20990-20992 NN denotes 4E
T17065 20993-20996 CC denotes and
T17066 20997-21001 NNS denotes data
T17068 21002-21005 RB denotes not
T17067 21006-21011 VBN denotes shown
T17069 21011-21012 -RRB- denotes )
T17070 21012-21013 . denotes .
T17071 21013-21200 sentence denotes This defect was suppressed in the Rb/E2f1 DKO retina, where we detected only 1 ± 1 dividing SAC, but not the Rb/E2f3 DKO retina, where there were 53 ± 8 dividing SACs (Figure 4D and 4E).
T17072 21014-21018 DT denotes This
T17073 21019-21025 NN denotes defect
T17075 21026-21029 VBD denotes was
T17074 21030-21040 VBN denotes suppressed
T17076 21041-21043 IN denotes in
T17077 21044-21047 DT denotes the
T17079 21048-21050 NN denotes Rb
T17081 21050-21051 HYPH denotes /
T17080 21051-21055 NN denotes E2f1
T17082 21056-21059 NN denotes DKO
T17078 21060-21066 NN denotes retina
T17083 21066-21068 , denotes ,
T17084 21068-21073 WRB denotes where
T17086 21074-21076 PRP denotes we
T17085 21077-21085 VBD denotes detected
T17087 21086-21090 RB denotes only
T17089 21091-21092 CD denotes 1
T17090 21093-21094 SYM denotes ±
T17088 21095-21096 CD denotes 1
T17092 21097-21105 NN denotes dividing
T17091 21106-21109 NN denotes SAC
T17093 21109-21111 , denotes ,
T17094 21111-21114 CC denotes but
T17095 21115-21118 RB denotes not
T17096 21119-21122 DT denotes the
T17098 21123-21125 NN denotes Rb
T17100 21125-21126 HYPH denotes /
T17099 21126-21130 NN denotes E2f3
T17101 21131-21134 NN denotes DKO
T17097 21135-21141 NN denotes retina
T17102 21141-21143 , denotes ,
T17103 21143-21148 WRB denotes where
T17105 21149-21154 EX denotes there
T17104 21155-21159 VBD denotes were
T17106 21160-21162 CD denotes 53
T17108 21163-21164 SYM denotes ±
T17107 21165-21166 CD denotes 8
T17110 21167-21175 VBG denotes dividing
T17109 21176-21180 NNS denotes SACs
T17111 21181-21182 -LRB- denotes (
T17113 21182-21188 NN denotes Figure
T17112 21189-21191 NN denotes 4D
T17114 21192-21195 CC denotes and
T17115 21196-21198 NN denotes 4E
T17116 21198-21199 -RRB- denotes )
T17117 21199-21200 . denotes .
T17118 21200-21314 sentence denotes We observed similar effects at P0 with Calb2, which marks newborn SACs and other amacrine cells (data not shown).
T17119 21201-21203 PRP denotes We
T17120 21204-21212 VBD denotes observed
T17121 21213-21220 JJ denotes similar
T17122 21221-21228 NNS denotes effects
T17123 21229-21231 IN denotes at
T17124 21232-21234 NN denotes P0
T17125 21235-21239 IN denotes with
T17126 21240-21245 NN denotes Calb2
T17127 21245-21247 , denotes ,
T17128 21247-21252 WDT denotes which
T17129 21253-21258 VBZ denotes marks
T17130 21259-21266 JJ denotes newborn
T17131 21267-21271 NNS denotes SACs
T17132 21272-21275 CC denotes and
T17133 21276-21281 JJ denotes other
T17135 21282-21290 JJ denotes amacrine
T17134 21291-21296 NNS denotes cells
T17136 21297-21298 -LRB- denotes (
T17138 21298-21302 NNS denotes data
T17139 21303-21306 RB denotes not
T17137 21307-21312 VBN denotes shown
T17140 21312-21313 -RRB- denotes )
T17141 21313-21314 . denotes .
T17142 21314-21495 sentence denotes Thus, in Rb KO SACs, E2f1 deletion suppresses ectopic division but not aberrant differentiation, whereas E2f3 deletion suppresses aberrant differentiation but not ectopic division.
T17143 21315-21319 RB denotes Thus
T17145 21319-21321 , denotes ,
T17146 21321-21323 IN denotes in
T17147 21324-21326 NN denotes Rb
T17148 21327-21329 NN denotes KO
T17149 21330-21334 NNS denotes SACs
T17150 21334-21336 , denotes ,
T17151 21336-21340 NN denotes E2f1
T17152 21341-21349 NN denotes deletion
T17144 21350-21360 VBZ denotes suppresses
T17153 21361-21368 JJ denotes ectopic
T17154 21369-21377 NN denotes division
T17155 21378-21381 CC denotes but
T17156 21382-21385 RB denotes not
T17157 21386-21394 JJ denotes aberrant
T17158 21395-21410 NN denotes differentiation
T17159 21410-21412 , denotes ,
T17160 21412-21419 IN denotes whereas
T17162 21420-21424 NN denotes E2f3
T17163 21425-21433 NN denotes deletion
T17161 21434-21444 VBZ denotes suppresses
T17164 21445-21453 JJ denotes aberrant
T17165 21454-21469 NN denotes differentiation
T17166 21470-21473 CC denotes but
T17167 21474-21477 RB denotes not
T17168 21478-21485 JJ denotes ectopic
T17169 21486-21494 NN denotes division
T17170 21494-21495 . denotes .
T18365 21497-21505 JJ denotes Specific
T18366 21506-21516 NN denotes Expression
T18367 21517-21519 IN denotes of
T18368 21520-21524 NN denotes E2f3
T18369 21525-21527 IN denotes in
T18370 21528-21532 NNS denotes SACs
T18371 21533-21536 CC denotes and
T18372 21537-21542 JJ denotes Other
T18373 21543-21550 NNS denotes Subsets
T18374 21551-21553 IN denotes of
T18375 21554-21557 NN denotes CNS
T18376 21558-21565 NNS denotes Neurons
T18377 21565-21749 sentence denotes The unique effect of E2f3 in disrupting the differentiation of SACs but not other retinal neurons might be due to cell-type-specific expression or cell-type-specific activity of E2f3.
T18378 21566-21569 DT denotes The
T18380 21570-21576 JJ denotes unique
T18379 21577-21583 NN denotes effect
T18382 21584-21586 IN denotes of
T18383 21587-21591 NN denotes E2f3
T18384 21592-21594 IN denotes in
T18385 21595-21605 VBG denotes disrupting
T18386 21606-21609 DT denotes the
T18387 21610-21625 NN denotes differentiation
T18388 21626-21628 IN denotes of
T18389 21629-21633 NNS denotes SACs
T18390 21634-21637 CC denotes but
T18391 21638-21641 RB denotes not
T18392 21642-21647 JJ denotes other
T18394 21648-21655 JJ denotes retinal
T18393 21656-21663 NNS denotes neurons
T18395 21664-21669 MD denotes might
T18381 21670-21672 VB denotes be
T18396 21673-21676 IN denotes due
T18397 21677-21679 IN denotes to
T18398 21680-21684 NN denotes cell
T18400 21684-21685 HYPH denotes -
T18399 21685-21689 NN denotes type
T18402 21689-21690 HYPH denotes -
T18401 21690-21698 JJ denotes specific
T18403 21699-21709 NN denotes expression
T18404 21710-21712 CC denotes or
T18405 21713-21717 NN denotes cell
T18407 21717-21718 HYPH denotes -
T18406 21718-21722 NN denotes type
T18409 21722-21723 HYPH denotes -
T18408 21723-21731 JJ denotes specific
T18410 21732-21740 NN denotes activity
T18411 21741-21743 IN denotes of
T18412 21744-21748 NN denotes E2f3
T18413 21748-21749 . denotes .
T18414 21749-21861 sentence denotes Determining between these two possibilities is not easy, as E2f immunostaining in mouse tissues is problematic.
T18415 21750-21761 VBG denotes Determining
T18417 21762-21769 IN denotes between
T18418 21770-21775 DT denotes these
T18420 21776-21779 CD denotes two
T18419 21780-21793 NNS denotes possibilities
T18416 21794-21796 VBZ denotes is
T18421 21797-21800 RB denotes not
T18422 21801-21805 JJ denotes easy
T18423 21805-21807 , denotes ,
T18424 21807-21809 IN denotes as
T18426 21810-21813 NN denotes E2f
T18427 21814-21828 NN denotes immunostaining
T18428 21829-21831 IN denotes in
T18429 21832-21837 NN denotes mouse
T18430 21838-21845 NNS denotes tissues
T18425 21846-21848 VBZ denotes is
T18431 21849-21860 JJ denotes problematic
T18432 21860-21861 . denotes .
T18433 21861-21991 sentence denotes We did not solve this issue for E2f1 or E2f2, but used a modified protocol [50] to successfully track E2f3 expression (Figure 5).
T18434 21862-21864 PRP denotes We
T18436 21865-21868 VBD denotes did
T18437 21869-21872 RB denotes not
T18435 21873-21878 VB denotes solve
T18438 21879-21883 DT denotes this
T18439 21884-21889 NN denotes issue
T18440 21890-21893 IN denotes for
T18441 21894-21898 NN denotes E2f1
T18442 21899-21901 CC denotes or
T18443 21902-21906 NN denotes E2f2
T18444 21906-21908 , denotes ,
T18445 21908-21911 CC denotes but
T18446 21912-21916 VBD denotes used
T18447 21917-21918 DT denotes a
T18449 21919-21927 VBN denotes modified
T18448 21928-21936 NN denotes protocol
T18450 21937-21938 -LRB- denotes [
T18451 21938-21940 CD denotes 50
T18452 21940-21941 -RRB- denotes ]
T18453 21942-21944 TO denotes to
T18455 21945-21957 RB denotes successfully
T18454 21958-21963 VB denotes track
T18456 21964-21968 NN denotes E2f3
T18457 21969-21979 NN denotes expression
T18458 21980-21981 -LRB- denotes (
T18459 21981-21987 NN denotes Figure
T18460 21988-21989 CD denotes 5
T18461 21989-21990 -RRB- denotes )
T18462 21990-21991 . denotes .
T18463 21991-22094 sentence denotes At P0, E2f3 was detected in RPCs, consistent with a putative role in normal proliferation (Figure 5A).
T18464 21992-21994 IN denotes At
T18466 21995-21997 NN denotes P0
T18467 21997-21999 , denotes ,
T18468 21999-22003 NN denotes E2f3
T18469 22004-22007 VBD denotes was
T18465 22008-22016 VBN denotes detected
T18470 22017-22019 IN denotes in
T18471 22020-22024 NNS denotes RPCs
T18472 22024-22026 , denotes ,
T18473 22026-22036 JJ denotes consistent
T18474 22037-22041 IN denotes with
T18475 22042-22043 DT denotes a
T18477 22044-22052 JJ denotes putative
T18476 22053-22057 NN denotes role
T18478 22058-22060 IN denotes in
T18479 22061-22067 JJ denotes normal
T18480 22068-22081 NN denotes proliferation
T18481 22082-22083 -LRB- denotes (
T18483 22083-22089 NN denotes Figure
T18482 22090-22092 NN denotes 5A
T18484 22092-22093 -RRB- denotes )
T18485 22093-22094 . denotes .
T18486 22094-22181 sentence denotes The signal was specific as it was absent in the E2f3 KO peripheral retina (Figure 5A).
T18487 22095-22098 DT denotes The
T18488 22099-22105 NN denotes signal
T18489 22106-22109 VBD denotes was
T18490 22110-22118 JJ denotes specific
T18491 22119-22121 IN denotes as
T18493 22122-22124 PRP denotes it
T18492 22125-22128 VBD denotes was
T18494 22129-22135 JJ denotes absent
T18495 22136-22138 IN denotes in
T18496 22139-22142 DT denotes the
T18498 22143-22147 NN denotes E2f3
T18499 22148-22150 NN denotes KO
T18500 22151-22161 JJ denotes peripheral
T18497 22162-22168 NN denotes retina
T18501 22169-22170 -LRB- denotes (
T18503 22170-22176 NN denotes Figure
T18502 22177-22179 NN denotes 5A
T18504 22179-22180 -RRB- denotes )
T18505 22180-22181 . denotes .
T18506 22181-22407 sentence denotes As the retina differentiated and RPC division diminished, the number of E2f3+ cells also dropped, and by P8, when division is virtually over, only a subset of post-mitotic cells in the inner retina expressed E2f3 (Figure 5A).
T18507 22182-22184 IN denotes As
T18509 22185-22188 DT denotes the
T18510 22189-22195 NN denotes retina
T18508 22196-22210 VBN denotes differentiated
T18512 22211-22214 CC denotes and
T18513 22215-22218 NN denotes RPC
T18514 22219-22227 NN denotes division
T18515 22228-22238 VBD denotes diminished
T18516 22238-22240 , denotes ,
T18517 22240-22243 DT denotes the
T18518 22244-22250 NN denotes number
T18519 22251-22253 IN denotes of
T18520 22254-22258 NN denotes E2f3
T18522 22258-22259 SYM denotes +
T18521 22260-22265 NNS denotes cells
T18523 22266-22270 RB denotes also
T18511 22271-22278 VBD denotes dropped
T18524 22278-22280 , denotes ,
T18525 22280-22283 CC denotes and
T18526 22284-22286 IN denotes by
T18528 22287-22289 NN denotes P8
T18529 22289-22291 , denotes ,
T18530 22291-22295 WRB denotes when
T18532 22296-22304 NN denotes division
T18531 22305-22307 VBZ denotes is
T18533 22308-22317 RB denotes virtually
T18534 22318-22322 RB denotes over
T18535 22322-22324 , denotes ,
T18536 22324-22328 RB denotes only
T18538 22329-22330 DT denotes a
T18537 22331-22337 NN denotes subset
T18539 22338-22340 IN denotes of
T18540 22341-22353 JJ denotes post-mitotic
T18541 22354-22359 NNS denotes cells
T18542 22360-22362 IN denotes in
T18543 22363-22366 DT denotes the
T18545 22367-22372 JJ denotes inner
T18544 22373-22379 NN denotes retina
T18527 22380-22389 VBD denotes expressed
T18546 22390-22394 NN denotes E2f3
T18547 22395-22396 -LRB- denotes (
T18549 22396-22402 NN denotes Figure
T18548 22403-22405 NN denotes 5A
T18550 22405-22406 -RRB- denotes )
T18551 22406-22407 . denotes .
T18552 22407-22559 sentence denotes By P18, E2f3 was also detected in two tracks in the IPL (Figure 5A and 5B), reminiscent of SAC markers such as Chat and Slc18a3 (c.f. Figures 3 and 4).
T18553 22408-22410 IN denotes By
T18555 22411-22414 NN denotes P18
T18556 22414-22416 , denotes ,
T18557 22416-22420 NN denotes E2f3
T18558 22421-22424 VBD denotes was
T18559 22425-22429 RB denotes also
T18554 22430-22438 VBN denotes detected
T18560 22439-22441 IN denotes in
T18561 22442-22445 CD denotes two
T18562 22446-22452 NNS denotes tracks
T18563 22453-22455 IN denotes in
T18564 22456-22459 DT denotes the
T18565 22460-22463 NN denotes IPL
T18566 22464-22465 -LRB- denotes (
T18568 22465-22471 NN denotes Figure
T18567 22472-22474 NN denotes 5A
T18569 22475-22478 CC denotes and
T18570 22479-22481 NN denotes 5B
T18571 22481-22482 -RRB- denotes )
T18572 22482-22484 , denotes ,
T18573 22484-22495 JJ denotes reminiscent
T18574 22496-22498 IN denotes of
T18575 22499-22502 NN denotes SAC
T18576 22503-22510 NNS denotes markers
T18577 22511-22515 JJ denotes such
T18578 22516-22518 IN denotes as
T18579 22519-22523 NN denotes Chat
T18580 22524-22527 CC denotes and
T18581 22528-22535 NN denotes Slc18a3
T18582 22536-22537 -LRB- denotes (
T18584 22537-22541 FW denotes c.f.
T18585 22542-22549 NNS denotes Figures
T18583 22550-22551 CD denotes 3
T18586 22552-22555 CC denotes and
T18587 22556-22557 CD denotes 4
T18588 22557-22558 -RRB- denotes )
T18589 22558-22559 . denotes .
T18590 22559-22700 sentence denotes This cytoplasmic E2f3 staining was also specific, as it was absent in the E2f3 KO peripheral retina of α-Cre;E2f3loxP/loxP mice (Figure 5A).
T18591 22560-22564 DT denotes This
T18593 22565-22576 JJ denotes cytoplasmic
T18594 22577-22581 NN denotes E2f3
T18592 22582-22590 NN denotes staining
T18595 22591-22594 VBD denotes was
T18596 22595-22599 RB denotes also
T18597 22600-22608 JJ denotes specific
T18598 22608-22610 , denotes ,
T18599 22610-22612 IN denotes as
T18601 22613-22615 PRP denotes it
T18600 22616-22619 VBD denotes was
T18602 22620-22626 JJ denotes absent
T18603 22627-22629 IN denotes in
T18604 22630-22633 DT denotes the
T18606 22634-22638 NN denotes E2f3
T18607 22639-22641 NN denotes KO
T18608 22642-22652 JJ denotes peripheral
T18605 22653-22659 NN denotes retina
T18609 22660-22662 IN denotes of
T18610 22663-22664 NN denotes α
T18612 22664-22665 HYPH denotes -
T18611 22665-22668 NN denotes Cre
T18614 22668-22669 : denotes ;
T18615 22669-22677 NN denotes E2f3loxP
T18617 22677-22678 HYPH denotes /
T18616 22678-22682 NN denotes loxP
T18613 22683-22687 NNS denotes mice
T18618 22688-22689 -LRB- denotes (
T18620 22689-22695 NN denotes Figure
T18619 22696-22698 NN denotes 5A
T18621 22698-22699 -RRB- denotes )
T18622 22699-22700 . denotes .
T18623 22700-22846 sentence denotes Indeed, double labelling with E2f3 (red) and Chat plus Slc18a3 (green) confirmed that E2f3 is present in both SAC soma and dendrites (Figure 5B).
T18624 22701-22707 RB denotes Indeed
T18626 22707-22709 , denotes ,
T18627 22709-22715 JJ denotes double
T18628 22716-22725 NN denotes labelling
T18629 22726-22730 IN denotes with
T18630 22731-22735 NN denotes E2f3
T18631 22736-22737 -LRB- denotes (
T18632 22737-22740 JJ denotes red
T18633 22740-22741 -RRB- denotes )
T18634 22742-22745 CC denotes and
T18635 22746-22750 NN denotes Chat
T18636 22751-22755 CC denotes plus
T18637 22756-22763 NN denotes Slc18a3
T18638 22764-22765 -LRB- denotes (
T18639 22765-22770 JJ denotes green
T18640 22770-22771 -RRB- denotes )
T18625 22772-22781 VBD denotes confirmed
T18641 22782-22786 IN denotes that
T18643 22787-22791 NN denotes E2f3
T18642 22792-22794 VBZ denotes is
T18644 22795-22802 JJ denotes present
T18645 22803-22805 IN denotes in
T18646 22806-22810 CC denotes both
T18648 22811-22814 NN denotes SAC
T18647 22815-22819 NN denotes soma
T18649 22820-22823 CC denotes and
T18650 22824-22833 NNS denotes dendrites
T18651 22834-22835 -LRB- denotes (
T18653 22835-22841 NN denotes Figure
T18652 22842-22844 NN denotes 5B
T18654 22844-22845 -RRB- denotes )
T18655 22845-22846 . denotes .
T18656 22846-23052 sentence denotes Rb protein was also detected in the inner retina (Figure 5A), and showed a similar distribution as E2f3 in SACs (Figure 5B), and was also present in mature ganglion cells and Müller cells as reported [51].
T18657 22847-22849 NN denotes Rb
T18658 22850-22857 NN denotes protein
T18660 22858-22861 VBD denotes was
T18661 22862-22866 RB denotes also
T18659 22867-22875 VBN denotes detected
T18662 22876-22878 IN denotes in
T18663 22879-22882 DT denotes the
T18665 22883-22888 JJ denotes inner
T18664 22889-22895 NN denotes retina
T18666 22896-22897 -LRB- denotes (
T18668 22897-22903 NN denotes Figure
T18667 22904-22906 NN denotes 5A
T18669 22906-22907 -RRB- denotes )
T18670 22907-22909 , denotes ,
T18671 22909-22912 CC denotes and
T18672 22913-22919 VBD denotes showed
T18673 22920-22921 DT denotes a
T18675 22922-22929 JJ denotes similar
T18674 22930-22942 NN denotes distribution
T18676 22943-22945 IN denotes as
T18677 22946-22950 NN denotes E2f3
T18678 22951-22953 IN denotes in
T18679 22954-22958 NNS denotes SACs
T18680 22959-22960 -LRB- denotes (
T18682 22960-22966 NN denotes Figure
T18681 22967-22969 NN denotes 5B
T18683 22969-22970 -RRB- denotes )
T18684 22970-22972 , denotes ,
T18685 22972-22975 CC denotes and
T18686 22976-22979 VBD denotes was
T18687 22980-22984 RB denotes also
T18688 22985-22992 JJ denotes present
T18689 22993-22995 IN denotes in
T18690 22996-23002 JJ denotes mature
T18692 23003-23011 NN denotes ganglion
T18691 23012-23017 NNS denotes cells
T18693 23018-23021 CC denotes and
T18694 23022-23028 NN denotes Müller
T18695 23029-23034 NNS denotes cells
T18696 23035-23037 IN denotes as
T18697 23038-23046 VBN denotes reported
T18698 23047-23048 -LRB- denotes [
T18699 23048-23050 CD denotes 51
T18700 23050-23051 -RRB- denotes ]
T18701 23051-23052 . denotes .
T18702 23052-23174 sentence denotes Rb staining in SAC processes was specific as it was absent in the peripheral retina of αCre;RbloxP/loxP mice (Figure 5A).
T18703 23053-23055 NN denotes Rb
T18704 23056-23064 NN denotes staining
T18706 23065-23067 IN denotes in
T18707 23068-23071 NN denotes SAC
T18708 23072-23081 NNS denotes processes
T18705 23082-23085 VBD denotes was
T18709 23086-23094 JJ denotes specific
T18710 23095-23097 IN denotes as
T18712 23098-23100 PRP denotes it
T18711 23101-23104 VBD denotes was
T18713 23105-23111 JJ denotes absent
T18714 23112-23114 IN denotes in
T18715 23115-23118 DT denotes the
T18717 23119-23129 JJ denotes peripheral
T18716 23130-23136 NN denotes retina
T18718 23137-23139 IN denotes of
T18719 23140-23144 NN denotes αCre
T18721 23144-23145 : denotes ;
T18722 23145-23151 NN denotes RbloxP
T18723 23151-23152 HYPH denotes /
T18720 23152-23156 NN denotes loxP
T18724 23157-23161 NNS denotes mice
T18725 23162-23163 -LRB- denotes (
T18727 23163-23169 NN denotes Figure
T18726 23170-23172 NN denotes 5A
T18728 23172-23173 -RRB- denotes )
T18729 23173-23174 . denotes .
T18730 23174-23346 sentence denotes These data suggest that Rb and E2f3 colocalize in SACs and that E2f3 triggers defects in SAC differentiation because it is specifically expressed in these retinal neurons.
T18731 23175-23180 DT denotes These
T18732 23181-23185 NNS denotes data
T18733 23186-23193 VBP denotes suggest
T18734 23194-23198 IN denotes that
T18736 23199-23201 NN denotes Rb
T18737 23202-23205 CC denotes and
T18738 23206-23210 NN denotes E2f3
T18735 23211-23221 VBP denotes colocalize
T18739 23222-23224 IN denotes in
T18740 23225-23229 NNS denotes SACs
T18741 23230-23233 CC denotes and
T18742 23234-23238 IN denotes that
T18744 23239-23243 NN denotes E2f3
T18743 23244-23252 VBZ denotes triggers
T18745 23253-23260 NNS denotes defects
T18746 23261-23263 IN denotes in
T18747 23264-23267 NN denotes SAC
T18748 23268-23283 NN denotes differentiation
T18749 23284-23291 IN denotes because
T18751 23292-23294 PRP denotes it
T18752 23295-23297 VBZ denotes is
T18753 23298-23310 RB denotes specifically
T18750 23311-23320 VBN denotes expressed
T18754 23321-23323 IN denotes in
T18755 23324-23329 DT denotes these
T18757 23330-23337 JJ denotes retinal
T18756 23338-23345 NNS denotes neurons
T18758 23345-23346 . denotes .
T18759 23346-24268 sentence denotes Figure 5 E2f3 and Rb Expression in SACs (A) Left panels: horizontal P0, P8, and P18 retinal sections of the indicated genotypes were stained for E2f3 (red) and DAPI (blue). The arrow indicates the junction between the E2f3 null peripheral and WT central P0 retina. Note the absence of E2f3 protein in the peripheral E2f3 KO RPCs at P0 and in peripheral inner retinal neurons at P18. Far right panel: P18 retinal sections of the indicated genotypes were stained for Rb (red) and DAPI (blue). Note the absence of Rb protein in the peripheral Rb KO inner retinal neurons. (B) WT P18 retinal sections were stained for nuclei (DAPI, blue), E2f3 (red) or Rb (red), and Chat plus Slc18a3 (green). Arrows indicate double-labelled soma. Note that the IPL processes are also double-labelled. Scale bars are 50 μm. We also found that E2f3 is present in a specific subset of mature neurons in various brain regions (data not shown).
T36780 23357-23361 NN denotes E2f3
T36782 23362-23365 CC denotes and
T36783 23366-23368 NN denotes Rb
T36781 23369-23379 NN denotes Expression
T36784 23380-23382 IN denotes in
T36785 23383-23387 NNS denotes SACs
T36786 23387-23520 sentence denotes (A) Left panels: horizontal P0, P8, and P18 retinal sections of the indicated genotypes were stained for E2f3 (red) and DAPI (blue).
T36787 23388-23389 -LRB- denotes (
T36788 23389-23390 LS denotes A
T36790 23390-23391 -RRB- denotes )
T36791 23392-23396 JJ denotes Left
T36792 23397-23403 NNS denotes panels
T36793 23403-23405 : denotes :
T36794 23405-23415 JJ denotes horizontal
T36796 23416-23418 NN denotes P0
T36797 23418-23420 , denotes ,
T36798 23420-23422 NN denotes P8
T36799 23422-23424 , denotes ,
T36800 23424-23427 CC denotes and
T36801 23428-23431 NN denotes P18
T36802 23432-23439 JJ denotes retinal
T36795 23440-23448 NNS denotes sections
T36803 23449-23451 IN denotes of
T36804 23452-23455 DT denotes the
T36806 23456-23465 VBN denotes indicated
T36805 23466-23475 NNS denotes genotypes
T36807 23476-23480 VBD denotes were
T36789 23481-23488 VBN denotes stained
T36808 23489-23492 IN denotes for
T36809 23493-23497 NN denotes E2f3
T36810 23498-23499 -LRB- denotes (
T36811 23499-23502 JJ denotes red
T36812 23502-23503 -RRB- denotes )
T36813 23504-23507 CC denotes and
T36814 23508-23512 NN denotes DAPI
T36815 23513-23514 -LRB- denotes (
T36816 23514-23518 JJ denotes blue
T36817 23518-23519 -RRB- denotes )
T36818 23519-23520 . denotes .
T36819 23520-23612 sentence denotes The arrow indicates the junction between the E2f3 null peripheral and WT central P0 retina.
T36820 23521-23524 DT denotes The
T36821 23525-23530 NN denotes arrow
T36822 23531-23540 VBZ denotes indicates
T36823 23541-23544 DT denotes the
T36824 23545-23553 NN denotes junction
T36825 23554-23561 IN denotes between
T36826 23562-23565 DT denotes the
T36828 23566-23570 NN denotes E2f3
T36829 23571-23575 JJ denotes null
T36827 23576-23586 JJ denotes peripheral
T36830 23587-23590 CC denotes and
T36831 23591-23593 NN denotes WT
T36833 23594-23601 JJ denotes central
T36834 23602-23604 NN denotes P0
T36832 23605-23611 NN denotes retina
T36835 23611-23612 . denotes .
T36836 23612-23730 sentence denotes Note the absence of E2f3 protein in the peripheral E2f3 KO RPCs at P0 and in peripheral inner retinal neurons at P18.
T36837 23613-23617 VB denotes Note
T36838 23618-23621 DT denotes the
T36839 23622-23629 NN denotes absence
T36840 23630-23632 IN denotes of
T36841 23633-23637 NN denotes E2f3
T36842 23638-23645 NN denotes protein
T36843 23646-23648 IN denotes in
T36844 23649-23652 DT denotes the
T36846 23653-23663 JJ denotes peripheral
T36847 23664-23668 NN denotes E2f3
T36848 23669-23671 NN denotes KO
T36845 23672-23676 NNS denotes RPCs
T36849 23677-23679 IN denotes at
T36850 23680-23682 NN denotes P0
T36851 23683-23686 CC denotes and
T36852 23687-23689 IN denotes in
T36854 23690-23700 JJ denotes peripheral
T36856 23701-23706 JJ denotes inner
T36857 23707-23714 JJ denotes retinal
T36855 23715-23722 NNS denotes neurons
T36853 23723-23725 IN denotes at
T36858 23726-23729 NN denotes P18
T36859 23729-23730 . denotes .
T36860 23730-23838 sentence denotes Far right panel: P18 retinal sections of the indicated genotypes were stained for Rb (red) and DAPI (blue).
T36861 23731-23734 RB denotes Far
T36862 23735-23740 JJ denotes right
T36863 23741-23746 NN denotes panel
T36865 23746-23748 : denotes :
T36866 23748-23751 NN denotes P18
T36868 23752-23759 JJ denotes retinal
T36867 23760-23768 NNS denotes sections
T36869 23769-23771 IN denotes of
T36870 23772-23775 DT denotes the
T36872 23776-23785 VBN denotes indicated
T36871 23786-23795 NNS denotes genotypes
T36873 23796-23800 VBD denotes were
T36864 23801-23808 VBN denotes stained
T36874 23809-23812 IN denotes for
T36875 23813-23815 NN denotes Rb
T36876 23816-23817 -LRB- denotes (
T36877 23817-23820 JJ denotes red
T36878 23820-23821 -RRB- denotes )
T36879 23822-23825 CC denotes and
T36880 23826-23830 NN denotes DAPI
T36881 23831-23832 -LRB- denotes (
T36882 23832-23836 JJ denotes blue
T36883 23836-23837 -RRB- denotes )
T36884 23837-23838 . denotes .
T36885 23838-23916 sentence denotes Note the absence of Rb protein in the peripheral Rb KO inner retinal neurons.
T36886 23839-23843 VB denotes Note
T36887 23844-23847 DT denotes the
T36888 23848-23855 NN denotes absence
T36889 23856-23858 IN denotes of
T36890 23859-23861 NN denotes Rb
T36891 23862-23869 NN denotes protein
T36892 23870-23872 IN denotes in
T36893 23873-23876 DT denotes the
T36895 23877-23887 JJ denotes peripheral
T36896 23888-23890 NN denotes Rb
T36897 23891-23893 NN denotes KO
T36898 23894-23899 JJ denotes inner
T36899 23900-23907 JJ denotes retinal
T36894 23908-23915 NNS denotes neurons
T36900 23915-23916 . denotes .
T36901 23916-24037 sentence denotes (B) WT P18 retinal sections were stained for nuclei (DAPI, blue), E2f3 (red) or Rb (red), and Chat plus Slc18a3 (green).
T36902 23917-23918 -LRB- denotes (
T36903 23918-23919 LS denotes B
T36905 23919-23920 -RRB- denotes )
T36906 23921-23923 NN denotes WT
T36908 23924-23927 NN denotes P18
T36909 23928-23935 JJ denotes retinal
T36907 23936-23944 NNS denotes sections
T36910 23945-23949 VBD denotes were
T36904 23950-23957 VBN denotes stained
T36911 23958-23961 IN denotes for
T36912 23962-23968 NNS denotes nuclei
T36913 23969-23970 -LRB- denotes (
T36915 23970-23974 NN denotes DAPI
T36916 23974-23976 , denotes ,
T36914 23976-23980 JJ denotes blue
T36917 23980-23981 -RRB- denotes )
T36918 23981-23983 , denotes ,
T36919 23983-23987 NN denotes E2f3
T36920 23988-23989 -LRB- denotes (
T36921 23989-23992 JJ denotes red
T36922 23992-23993 -RRB- denotes )
T36923 23994-23996 CC denotes or
T36924 23997-23999 NN denotes Rb
T36925 24000-24001 -LRB- denotes (
T36926 24001-24004 JJ denotes red
T36927 24004-24005 -RRB- denotes )
T36928 24005-24007 , denotes ,
T36929 24007-24010 CC denotes and
T36930 24011-24015 NN denotes Chat
T36931 24016-24020 CC denotes plus
T36932 24021-24028 NN denotes Slc18a3
T36933 24029-24030 -LRB- denotes (
T36934 24030-24035 JJ denotes green
T36935 24035-24036 -RRB- denotes )
T36936 24036-24037 . denotes .
T36937 24037-24075 sentence denotes Arrows indicate double-labelled soma.
T36938 24038-24044 NNS denotes Arrows
T36939 24045-24053 VBP denotes indicate
T36940 24054-24060 JJ denotes double
T36942 24060-24061 HYPH denotes -
T36941 24061-24069 VBN denotes labelled
T36943 24070-24074 NNS denotes soma
T36944 24074-24075 . denotes .
T36945 24075-24129 sentence denotes Note that the IPL processes are also double-labelled.
T36946 24076-24080 VB denotes Note
T36947 24081-24085 IN denotes that
T36949 24086-24089 DT denotes the
T36951 24090-24093 NN denotes IPL
T36950 24094-24103 NNS denotes processes
T36952 24104-24107 VBP denotes are
T36953 24108-24112 RB denotes also
T36954 24113-24119 JJ denotes double
T36955 24119-24120 HYPH denotes -
T36948 24120-24128 VBN denotes labelled
T36956 24128-24129 . denotes .
T36957 24129-24151 sentence denotes Scale bars are 50 μm.
T36958 24130-24135 NN denotes Scale
T36959 24136-24140 NNS denotes bars
T36960 24141-24144 VBP denotes are
T36961 24145-24147 CD denotes 50
T36962 24148-24150 NN denotes μm
T36963 24150-24151 . denotes .
T18760 24152-24154 PRP denotes We
T18762 24155-24159 RB denotes also
T18761 24160-24165 VBD denotes found
T18763 24166-24170 IN denotes that
T18765 24171-24175 NN denotes E2f3
T18764 24176-24178 VBZ denotes is
T18766 24179-24186 JJ denotes present
T18767 24187-24189 IN denotes in
T18768 24190-24191 DT denotes a
T18770 24192-24200 JJ denotes specific
T18769 24201-24207 NN denotes subset
T18771 24208-24210 IN denotes of
T18772 24211-24217 JJ denotes mature
T18773 24218-24225 NNS denotes neurons
T18774 24226-24228 IN denotes in
T18775 24229-24236 JJ denotes various
T18777 24237-24242 NN denotes brain
T18776 24243-24250 NNS denotes regions
T18778 24251-24252 -LRB- denotes (
T18780 24252-24256 NNS denotes data
T18781 24257-24260 RB denotes not
T18779 24261-24266 VBN denotes shown
T18782 24266-24267 -RRB- denotes )
T18783 24267-24268 . denotes .
T18784 24268-24476 sentence denotes For example, in the P20 amygdala, E2f3 colocalized with the general neuronal markers Mtap2 and Mecp2 [52], but not with Calb2, which marks a subset of neurons, or with the glial marker Gfap (data not shown).
T18785 24269-24272 IN denotes For
T18787 24273-24280 NN denotes example
T18788 24280-24282 , denotes ,
T18789 24282-24284 IN denotes in
T18790 24285-24288 DT denotes the
T18792 24289-24292 NN denotes P20
T18791 24293-24301 NN denotes amygdala
T18793 24301-24303 , denotes ,
T18794 24303-24307 NN denotes E2f3
T18786 24308-24319 VBD denotes colocalized
T18795 24320-24324 IN denotes with
T18796 24325-24328 DT denotes the
T18798 24329-24336 JJ denotes general
T18799 24337-24345 JJ denotes neuronal
T18797 24346-24353 NNS denotes markers
T18800 24354-24359 NN denotes Mtap2
T18801 24360-24363 CC denotes and
T18802 24364-24369 NN denotes Mecp2
T18803 24370-24371 -LRB- denotes [
T18804 24371-24373 CD denotes 52
T18805 24373-24374 -RRB- denotes ]
T18806 24374-24376 , denotes ,
T18807 24376-24379 CC denotes but
T18808 24380-24383 RB denotes not
T18809 24384-24388 IN denotes with
T18810 24389-24394 NN denotes Calb2
T18811 24394-24396 , denotes ,
T18812 24396-24401 WDT denotes which
T18813 24402-24407 VBZ denotes marks
T18814 24408-24409 DT denotes a
T18815 24410-24416 NN denotes subset
T18816 24417-24419 IN denotes of
T18817 24420-24427 NNS denotes neurons
T18818 24427-24429 , denotes ,
T18819 24429-24431 CC denotes or
T18820 24432-24436 IN denotes with
T18821 24437-24440 DT denotes the
T18823 24441-24446 JJ denotes glial
T18822 24447-24453 NN denotes marker
T18824 24454-24458 NN denotes Gfap
T18825 24459-24460 -LRB- denotes (
T18827 24460-24464 NNS denotes data
T18828 24465-24468 RB denotes not
T18826 24469-24474 VBN denotes shown
T18829 24474-24475 -RRB- denotes )
T18830 24475-24476 . denotes .
T18831 24476-24640 sentence denotes Unlike in retinal SACs, E2f3 was not coexpressed in Chat+ or Slc18a3+ cholinergic neurons located in various regions of the brain and spinal cord (data not shown).
T18832 24477-24483 IN denotes Unlike
T18834 24484-24486 IN denotes in
T18835 24487-24494 JJ denotes retinal
T18836 24495-24499 NNS denotes SACs
T18837 24499-24501 , denotes ,
T18838 24501-24505 NN denotes E2f3
T18839 24506-24509 VBD denotes was
T18840 24510-24513 RB denotes not
T18833 24514-24525 VBN denotes coexpressed
T18841 24526-24528 IN denotes in
T18842 24529-24533 NN denotes Chat
T18844 24533-24534 SYM denotes +
T18845 24535-24537 CC denotes or
T18846 24538-24545 NN denotes Slc18a3
T18847 24545-24546 SYM denotes +
T18848 24547-24558 JJ denotes cholinergic
T18843 24559-24566 NNS denotes neurons
T18849 24567-24574 VBN denotes located
T18850 24575-24577 IN denotes in
T18851 24578-24585 JJ denotes various
T18852 24586-24593 NNS denotes regions
T18853 24594-24596 IN denotes of
T18854 24597-24600 DT denotes the
T18855 24601-24606 NN denotes brain
T18856 24607-24610 CC denotes and
T18857 24611-24617 JJ denotes spinal
T18858 24618-24622 NN denotes cord
T18859 24623-24624 -LRB- denotes (
T18861 24624-24628 NNS denotes data
T18862 24629-24632 RB denotes not
T18860 24633-24638 VBN denotes shown
T18863 24638-24639 -RRB- denotes )
T18864 24639-24640 . denotes .
T18865 24640-24851 sentence denotes In agreement, we could not detect defects in cholinergic Rb KO neurons in the developing forebrain, but other Rb KO neurons in this region showed differentiation defects that were rescued by deleting E2f3 [53].
T18866 24641-24643 IN denotes In
T18868 24644-24653 NN denotes agreement
T18869 24653-24655 , denotes ,
T18870 24655-24657 PRP denotes we
T18871 24658-24663 MD denotes could
T18872 24664-24667 RB denotes not
T18867 24668-24674 VB denotes detect
T18873 24675-24682 NNS denotes defects
T18874 24683-24685 IN denotes in
T18875 24686-24697 JJ denotes cholinergic
T18877 24698-24700 NN denotes Rb
T18878 24701-24703 NN denotes KO
T18876 24704-24711 NNS denotes neurons
T18879 24712-24714 IN denotes in
T18880 24715-24718 DT denotes the
T18882 24719-24729 VBG denotes developing
T18881 24730-24739 NN denotes forebrain
T18883 24739-24741 , denotes ,
T18884 24741-24744 CC denotes but
T18885 24745-24750 JJ denotes other
T18887 24751-24753 NN denotes Rb
T18888 24754-24756 NN denotes KO
T18886 24757-24764 NNS denotes neurons
T18890 24765-24767 IN denotes in
T18891 24768-24772 DT denotes this
T18892 24773-24779 NN denotes region
T18889 24780-24786 VBD denotes showed
T18893 24787-24802 NN denotes differentiation
T18894 24803-24810 NNS denotes defects
T18895 24811-24815 WDT denotes that
T18897 24816-24820 VBD denotes were
T18896 24821-24828 VBN denotes rescued
T18898 24829-24831 IN denotes by
T18899 24832-24840 VBG denotes deleting
T18900 24841-24845 NN denotes E2f3
T18901 24846-24847 -LRB- denotes [
T18902 24847-24849 CD denotes 53
T18903 24849-24850 -RRB- denotes ]
T18904 24850-24851 . denotes .
T18905 24851-24981 sentence denotes Together, these results suggest that the common mechanism by which Rb promotes neural differentiation is through E2f3 inhibition.
T18906 24852-24860 RB denotes Together
T18908 24860-24862 , denotes ,
T18909 24862-24867 DT denotes these
T18910 24868-24875 NNS denotes results
T18907 24876-24883 VBP denotes suggest
T18911 24884-24888 IN denotes that
T18913 24889-24892 DT denotes the
T18915 24893-24899 JJ denotes common
T18914 24900-24909 NN denotes mechanism
T18916 24910-24912 IN denotes by
T18918 24913-24918 WDT denotes which
T18919 24919-24921 NN denotes Rb
T18917 24922-24930 VBZ denotes promotes
T18920 24931-24937 JJ denotes neural
T18921 24938-24953 NN denotes differentiation
T18912 24954-24956 VBZ denotes is
T18922 24957-24964 IN denotes through
T18923 24965-24969 NN denotes E2f3
T18924 24970-24980 NN denotes inhibition
T18925 24980-24981 . denotes .
T20216 24983-24991 JJ denotes Distinct
T20217 24992-25004 NN denotes Localization
T20218 25005-25007 IN denotes of
T20219 25008-25012 NN denotes E2f3
T20220 25013-25021 NNS denotes Isoforms
T20221 25021-25119 sentence denotes As noted above, E2f3 and Rb staining in SACs was both nuclear and cytoplasmic (Figure 5A and 5B).
T20222 25022-25024 IN denotes As
T20223 25025-25030 VBN denotes noted
T20225 25031-25036 RB denotes above
T20226 25036-25038 , denotes ,
T20227 25038-25042 NN denotes E2f3
T20229 25043-25046 CC denotes and
T20230 25047-25049 NN denotes Rb
T20228 25050-25058 NN denotes staining
T20231 25059-25061 IN denotes in
T20232 25062-25066 NNS denotes SACs
T20224 25067-25070 VBD denotes was
T20233 25071-25075 CC denotes both
T20234 25076-25083 JJ denotes nuclear
T20235 25084-25087 CC denotes and
T20236 25088-25099 JJ denotes cytoplasmic
T20237 25100-25101 -LRB- denotes (
T20239 25101-25107 NN denotes Figure
T20238 25108-25110 NN denotes 5A
T20240 25111-25114 CC denotes and
T20241 25115-25117 NN denotes 5B
T20242 25117-25118 -RRB- denotes )
T20243 25118-25119 . denotes .
T20244 25119-25238 sentence denotes The antibody that worked in immunostaining recognizes a C-terminal region and thus, does not distinguish a/b isoforms.
T20245 25120-25123 DT denotes The
T20246 25124-25132 NN denotes antibody
T20248 25133-25137 WDT denotes that
T20249 25138-25144 VBD denotes worked
T20250 25145-25147 IN denotes in
T20251 25148-25162 NN denotes immunostaining
T20247 25163-25173 VBZ denotes recognizes
T20252 25174-25175 DT denotes a
T20254 25176-25177 NN denotes C
T20256 25177-25178 HYPH denotes -
T20255 25178-25186 JJ denotes terminal
T20253 25187-25193 NN denotes region
T20257 25194-25197 CC denotes and
T20258 25198-25202 RB denotes thus
T20260 25202-25204 , denotes ,
T20261 25204-25208 VBZ denotes does
T20262 25209-25212 RB denotes not
T20259 25213-25224 VB denotes distinguish
T20263 25225-25226 NN denotes a
T20265 25226-25227 HYPH denotes /
T20264 25227-25228 NN denotes b
T20266 25229-25237 NNS denotes isoforms
T20267 25237-25238 . denotes .
T20268 25238-25340 sentence denotes To our knowledge, the subcellular location of E2f3 isoforms has not been determined in any cell type.
T20269 25239-25241 IN denotes To
T20271 25242-25245 PRP$ denotes our
T20272 25246-25255 NN denotes knowledge
T20273 25255-25257 , denotes ,
T20274 25257-25260 DT denotes the
T20276 25261-25272 JJ denotes subcellular
T20275 25273-25281 NN denotes location
T20277 25282-25284 IN denotes of
T20278 25285-25289 NN denotes E2f3
T20279 25290-25298 NNS denotes isoforms
T20280 25299-25302 VBZ denotes has
T20281 25303-25306 RB denotes not
T20282 25307-25311 VBN denotes been
T20270 25312-25322 VBN denotes determined
T20283 25323-25325 IN denotes in
T20284 25326-25329 DT denotes any
T20286 25330-25334 NN denotes cell
T20285 25335-25339 NN denotes type
T20287 25339-25340 . denotes .
T20288 25340-25534 sentence denotes To verify the dual locations of E2f3 and to determine which isoforms were present in retina, we analyzed nuclear and cytoplasmic fractions by Western blot at different times during development.
T20289 25341-25343 TO denotes To
T20290 25344-25350 VB denotes verify
T20292 25351-25354 DT denotes the
T20294 25355-25359 JJ denotes dual
T20293 25360-25369 NNS denotes locations
T20295 25370-25372 IN denotes of
T20296 25373-25377 NN denotes E2f3
T20297 25378-25381 CC denotes and
T20298 25382-25384 TO denotes to
T20299 25385-25394 VB denotes determine
T20300 25395-25400 WDT denotes which
T20301 25401-25409 NNS denotes isoforms
T20302 25410-25414 VBD denotes were
T20303 25415-25422 JJ denotes present
T20304 25423-25425 IN denotes in
T20305 25426-25432 NN denotes retina
T20306 25432-25434 , denotes ,
T20307 25434-25436 PRP denotes we
T20291 25437-25445 VBD denotes analyzed
T20308 25446-25453 JJ denotes nuclear
T20310 25454-25457 CC denotes and
T20311 25458-25469 JJ denotes cytoplasmic
T20309 25470-25479 NNS denotes fractions
T20312 25480-25482 IN denotes by
T20313 25483-25490 NNP denotes Western
T20314 25491-25495 NN denotes blot
T20315 25496-25498 IN denotes at
T20316 25499-25508 JJ denotes different
T20317 25509-25514 NNS denotes times
T20318 25515-25521 IN denotes during
T20319 25522-25533 NN denotes development
T20320 25533-25534 . denotes .
T20321 25534-25678 sentence denotes Analysis with the pan-E2f3 antibody (sc-878, Santa Cruz Biotechnology) detected a 55-kD E2f3a species and a 40-kD E2f3b polypeptide (Figure 6).
T20322 25535-25543 NN denotes Analysis
T20324 25544-25548 IN denotes with
T20325 25549-25552 DT denotes the
T20327 25553-25561 JJ denotes pan-E2f3
T20326 25562-25570 NN denotes antibody
T20328 25571-25572 -LRB- denotes (
T20329 25572-25574 NN denotes sc
T20330 25574-25575 HYPH denotes -
T20331 25575-25578 CD denotes 878
T20332 25578-25580 , denotes ,
T20334 25580-25585 NNP denotes Santa
T20335 25586-25590 NNP denotes Cruz
T20333 25591-25604 NNP denotes Biotechnology
T20336 25604-25605 -RRB- denotes )
T20323 25606-25614 VBD denotes detected
T20337 25615-25616 DT denotes a
T20339 25617-25619 CD denotes 55
T20341 25619-25620 HYPH denotes -
T20340 25620-25622 NN denotes kD
T20342 25623-25628 NN denotes E2f3a
T20338 25629-25636 NNS denotes species
T20343 25637-25640 CC denotes and
T20344 25641-25642 DT denotes a
T20346 25643-25645 CD denotes 40
T20348 25645-25646 HYPH denotes -
T20347 25646-25648 NN denotes kD
T20349 25649-25654 NN denotes E2f3b
T20345 25655-25666 NN denotes polypeptide
T20350 25667-25668 -LRB- denotes (
T20351 25668-25674 NN denotes Figure
T20352 25675-25676 CD denotes 6
T20353 25676-25677 -RRB- denotes )
T20354 25677-25678 . denotes .
T20355 25678-25865 sentence denotes To confirm that the upper species in our retinal lysates was E2f3a, we exploited novel mice that lack E2f3 exon 1a and thus express E2f3b exclusively (R. O. and G. L., unpublished data).
T20356 25679-25681 TO denotes To
T20357 25682-25689 VB denotes confirm
T20359 25690-25694 IN denotes that
T20361 25695-25698 DT denotes the
T20363 25699-25704 JJ denotes upper
T20362 25705-25712 NNS denotes species
T20364 25713-25715 IN denotes in
T20365 25716-25719 PRP$ denotes our
T20367 25720-25727 JJ denotes retinal
T20366 25728-25735 NNS denotes lysates
T20360 25736-25739 VBD denotes was
T20368 25740-25745 NN denotes E2f3a
T20369 25745-25747 , denotes ,
T20370 25747-25749 PRP denotes we
T20358 25750-25759 VBD denotes exploited
T20371 25760-25765 JJ denotes novel
T20372 25766-25770 NNS denotes mice
T20373 25771-25775 WDT denotes that
T20374 25776-25780 VBP denotes lack
T20375 25781-25785 NN denotes E2f3
T20377 25786-25790 NN denotes exon
T20376 25791-25793 NN denotes 1a
T20378 25794-25797 CC denotes and
T20379 25798-25802 RB denotes thus
T20380 25803-25810 VBP denotes express
T20381 25811-25816 NN denotes E2f3b
T20382 25817-25828 RB denotes exclusively
T20383 25829-25830 -LRB- denotes (
T20384 25830-25832 NNP denotes R.
T20385 25833-25835 NNP denotes O.
T20386 25836-25839 CC denotes and
T20387 25840-25842 NNP denotes G.
T20388 25843-25845 NNP denotes L.
T20389 25845-25847 , denotes ,
T20390 25847-25858 JJ denotes unpublished
T20391 25859-25863 NNS denotes data
T20392 25863-25864 -RRB- denotes )
T20393 25864-25865 . denotes .
T20394 25865-25948 sentence denotes The genotyping strategy is discussed in detail later and is outlined in Figure 7A.
T20395 25866-25869 DT denotes The
T20397 25870-25880 NN denotes genotyping
T20396 25881-25889 NN denotes strategy
T20399 25890-25892 VBZ denotes is
T20398 25893-25902 VBN denotes discussed
T20400 25903-25905 IN denotes in
T20401 25906-25912 NN denotes detail
T20402 25913-25918 RB denotes later
T20403 25919-25922 CC denotes and
T20404 25923-25925 VBZ denotes is
T20405 25926-25934 VBN denotes outlined
T20406 25935-25937 IN denotes in
T20407 25938-25944 NN denotes Figure
T20408 25945-25947 NN denotes 7A
T20409 25947-25948 . denotes .
T20410 25948-26043 sentence denotes Western analysis confirmed that the upper band was absent in E2f3a−/− mice (Figures 6 and S8).
T20411 25949-25956 NNP denotes Western
T20412 25957-25965 NN denotes analysis
T20413 25966-25975 VBD denotes confirmed
T20414 25976-25980 IN denotes that
T20416 25981-25984 DT denotes the
T20418 25985-25990 JJ denotes upper
T20417 25991-25995 NN denotes band
T20415 25996-25999 VBD denotes was
T20419 26000-26006 JJ denotes absent
T20420 26007-26009 IN denotes in
T20421 26010-26015 NN denotes E2f3a
T20423 26015-26016 SYM denotes −
T20424 26016-26017 HYPH denotes /
T20425 26017-26018 SYM denotes −
T20422 26019-26023 NNS denotes mice
T20426 26024-26025 -LRB- denotes (
T20428 26025-26032 NNS denotes Figures
T20429 26033-26034 CD denotes 6
T20430 26035-26038 CC denotes and
T20427 26039-26041 NN denotes S8
T20431 26041-26042 -RRB- denotes )
T20432 26042-26043 . denotes .
T20433 26043-26204 sentence denotes Consistent with the drop in E2f3-expressing cells during WT retinal maturation (Figure 5A), the total amount of E2f3a was less at P18 compared to P0 (Figure 6).
T20434 26044-26054 JJ denotes Consistent
T20436 26055-26059 IN denotes with
T20437 26060-26063 DT denotes the
T20438 26064-26068 NN denotes drop
T20439 26069-26071 IN denotes in
T20440 26072-26076 NN denotes E2f3
T20442 26076-26077 HYPH denotes -
T20441 26077-26087 VBG denotes expressing
T20443 26088-26093 NNS denotes cells
T20444 26094-26100 IN denotes during
T20445 26101-26103 NN denotes WT
T20447 26104-26111 JJ denotes retinal
T20446 26112-26122 NN denotes maturation
T20448 26123-26124 -LRB- denotes (
T20450 26124-26130 NN denotes Figure
T20449 26131-26133 NN denotes 5A
T20451 26133-26134 -RRB- denotes )
T20452 26134-26136 , denotes ,
T20453 26136-26139 DT denotes the
T20455 26140-26145 JJ denotes total
T20454 26146-26152 NN denotes amount
T20456 26153-26155 IN denotes of
T20457 26156-26161 NN denotes E2f3a
T20435 26162-26165 VBD denotes was
T20458 26166-26170 JJR denotes less
T20459 26171-26173 IN denotes at
T20460 26174-26177 NN denotes P18
T20461 26178-26186 VBN denotes compared
T20462 26187-26189 IN denotes to
T20463 26190-26192 NN denotes P0
T20464 26193-26194 -LRB- denotes (
T20465 26194-26200 NN denotes Figure
T20466 26201-26202 CD denotes 6
T20467 26202-26203 -RRB- denotes )
T20468 26203-26204 . denotes .
T20469 26204-26262 sentence denotes E2f3b was present in similar amounts at both time points.
T20470 26205-26210 NN denotes E2f3b
T20471 26211-26214 VBD denotes was
T20472 26215-26222 JJ denotes present
T20473 26223-26225 IN denotes in
T20474 26226-26233 JJ denotes similar
T20475 26234-26241 NNS denotes amounts
T20476 26242-26244 IN denotes at
T20477 26245-26249 DT denotes both
T20479 26250-26254 NN denotes time
T20478 26255-26261 NNS denotes points
T20480 26261-26262 . denotes .
T20481 26262-26418 sentence denotes At P0 and P18, E2f3a was present in both nuclear and cytoplasmic fractions, but in marked contrast, E2f3b was exclusively nuclear at both times (Figure 6).
T20482 26263-26265 IN denotes At
T20484 26266-26268 NN denotes P0
T20485 26269-26272 CC denotes and
T20486 26273-26276 NN denotes P18
T20487 26276-26278 , denotes ,
T20488 26278-26283 NN denotes E2f3a
T20483 26284-26287 VBD denotes was
T20489 26288-26295 JJ denotes present
T20490 26296-26298 IN denotes in
T20491 26299-26303 CC denotes both
T20492 26304-26311 JJ denotes nuclear
T20494 26312-26315 CC denotes and
T20495 26316-26327 JJ denotes cytoplasmic
T20493 26328-26337 NNS denotes fractions
T20496 26337-26339 , denotes ,
T20497 26339-26342 CC denotes but
T20498 26343-26345 IN denotes in
T20500 26346-26352 JJ denotes marked
T20501 26353-26361 NN denotes contrast
T20502 26361-26363 , denotes ,
T20503 26363-26368 NN denotes E2f3b
T20499 26369-26372 VBD denotes was
T20504 26373-26384 RB denotes exclusively
T20505 26385-26392 JJ denotes nuclear
T20506 26393-26395 IN denotes at
T20507 26396-26400 DT denotes both
T20508 26401-26406 NNS denotes times
T20509 26407-26408 -LRB- denotes (
T20510 26408-26414 NN denotes Figure
T20511 26415-26416 CD denotes 6
T20512 26416-26417 -RRB- denotes )
T20513 26417-26418 . denotes .
T20514 26418-26619 sentence denotes Two closely migrating E2f3a bands were detected, more clearly evident at P18, of which the faster migrating species was dominant in nuclear and the slower species was dominant in cytoplasm (Figure 6).
T20515 26419-26422 CD denotes Two
T20517 26423-26430 RB denotes closely
T20518 26431-26440 VBG denotes migrating
T20519 26441-26446 NN denotes E2f3a
T20516 26447-26452 NNS denotes bands
T20521 26453-26457 VBD denotes were
T20520 26458-26466 VBN denotes detected
T20522 26466-26468 , denotes ,
T20523 26468-26472 RBR denotes more
T20524 26473-26480 RB denotes clearly
T20525 26481-26488 JJ denotes evident
T20526 26489-26491 IN denotes at
T20527 26492-26495 NN denotes P18
T20528 26495-26497 , denotes ,
T20529 26497-26499 IN denotes of
T20531 26500-26505 WDT denotes which
T20532 26506-26509 DT denotes the
T20534 26510-26516 RBR denotes faster
T20535 26517-26526 VBG denotes migrating
T20533 26527-26534 NN denotes species
T20530 26535-26538 VBD denotes was
T20536 26539-26547 JJ denotes dominant
T20537 26548-26550 IN denotes in
T20538 26551-26558 JJ denotes nuclear
T20539 26559-26562 CC denotes and
T20540 26563-26566 DT denotes the
T20542 26567-26573 JJR denotes slower
T20541 26574-26581 NN denotes species
T20543 26582-26585 VBD denotes was
T20544 26586-26594 JJ denotes dominant
T20545 26595-26597 IN denotes in
T20546 26598-26607 NN denotes cytoplasm
T20547 26608-26609 -LRB- denotes (
T20548 26609-26615 NN denotes Figure
T20549 26616-26617 CD denotes 6
T20550 26617-26618 -RRB- denotes )
T20551 26618-26619 . denotes .
T20552 26619-26728 sentence denotes The identity of both as E2f3a species was confirmed by their absence in the P18 E2f3a KO retina (Figure S8).
T20553 26620-26623 DT denotes The
T20554 26624-26632 NN denotes identity
T20556 26633-26635 IN denotes of
T20557 26636-26640 DT denotes both
T20558 26641-26643 IN denotes as
T20559 26644-26649 NN denotes E2f3a
T20560 26650-26657 NNS denotes species
T20561 26658-26661 VBD denotes was
T20555 26662-26671 VBN denotes confirmed
T20562 26672-26674 IN denotes by
T20563 26675-26680 PRP$ denotes their
T20564 26681-26688 NN denotes absence
T20565 26689-26691 IN denotes in
T20566 26692-26695 DT denotes the
T20568 26696-26699 NN denotes P18
T20569 26700-26705 NN denotes E2f3a
T20570 26706-26708 NN denotes KO
T20567 26709-26715 NN denotes retina
T20571 26716-26717 -LRB- denotes (
T20573 26717-26723 NN denotes Figure
T20572 26724-26726 NN denotes S8
T20574 26726-26727 -RRB- denotes )
T20575 26727-26728 . denotes .
T20576 26728-26996 sentence denotes Analysis of Pou4f2, a nuclear transcription factor expressed in ganglion cells, showed that nuclear proteins had not contaminated the cytoplasmic fraction, and analysis of Slc18a3, a cytoplasmic SAC marker, confirmed that the reverse had also not occurred (Figure 6).
T20577 26729-26737 NN denotes Analysis
T20579 26738-26740 IN denotes of
T20580 26741-26747 NN denotes Pou4f2
T20581 26747-26749 , denotes ,
T20582 26749-26750 DT denotes a
T20584 26751-26758 JJ denotes nuclear
T20585 26759-26772 NN denotes transcription
T20583 26773-26779 NN denotes factor
T20586 26780-26789 VBN denotes expressed
T20587 26790-26792 IN denotes in
T20588 26793-26801 NN denotes ganglion
T20589 26802-26807 NNS denotes cells
T20590 26807-26809 , denotes ,
T20578 26809-26815 VBD denotes showed
T20591 26816-26820 IN denotes that
T20593 26821-26828 JJ denotes nuclear
T20594 26829-26837 NN denotes proteins
T20595 26838-26841 VBD denotes had
T20596 26842-26845 RB denotes not
T20592 26846-26858 VBN denotes contaminated
T20597 26859-26862 DT denotes the
T20599 26863-26874 JJ denotes cytoplasmic
T20598 26875-26883 NN denotes fraction
T20600 26883-26885 , denotes ,
T20601 26885-26888 CC denotes and
T20602 26889-26897 NN denotes analysis
T20604 26898-26900 IN denotes of
T20605 26901-26908 NN denotes Slc18a3
T20606 26908-26910 , denotes ,
T20607 26910-26911 DT denotes a
T20609 26912-26923 JJ denotes cytoplasmic
T20610 26924-26927 NN denotes SAC
T20608 26928-26934 NN denotes marker
T20611 26934-26936 , denotes ,
T20603 26936-26945 VBD denotes confirmed
T20612 26946-26950 IN denotes that
T20614 26951-26954 DT denotes the
T20615 26955-26962 NN denotes reverse
T20616 26963-26966 VBD denotes had
T20617 26967-26971 RB denotes also
T20618 26972-26975 RB denotes not
T20613 26976-26984 VBN denotes occurred
T20619 26985-26986 -LRB- denotes (
T20620 26986-26992 NN denotes Figure
T20621 26993-26994 CD denotes 6
T20622 26994-26995 -RRB- denotes )
T20623 26995-26996 . denotes .
T20624 26996-27245 sentence denotes These data show, to our knowledge for the first time, that E2f3a and E2f3b exhibit distinct patterns of subcellular distribution, and raise the possibility that E2f3a localization may be regulated by as yet unknown post-translational modifications.
T20625 26997-27002 DT denotes These
T20626 27003-27007 NNS denotes data
T20627 27008-27012 VBP denotes show
T20628 27012-27014 , denotes ,
T20629 27014-27016 IN denotes to
T20631 27017-27020 PRP$ denotes our
T20632 27021-27030 NN denotes knowledge
T20630 27031-27034 IN denotes for
T20633 27035-27038 DT denotes the
T20635 27039-27044 JJ denotes first
T20634 27045-27049 NN denotes time
T20636 27049-27051 , denotes ,
T20637 27051-27055 IN denotes that
T20639 27056-27061 NN denotes E2f3a
T20640 27062-27065 CC denotes and
T20641 27066-27071 NN denotes E2f3b
T20638 27072-27079 VBP denotes exhibit
T20642 27080-27088 JJ denotes distinct
T20643 27089-27097 NNS denotes patterns
T20644 27098-27100 IN denotes of
T20645 27101-27112 JJ denotes subcellular
T20646 27113-27125 NN denotes distribution
T20647 27125-27127 , denotes ,
T20648 27127-27130 CC denotes and
T20649 27131-27136 VB denotes raise
T20650 27137-27140 DT denotes the
T20651 27141-27152 NN denotes possibility
T20652 27153-27157 IN denotes that
T20654 27158-27163 NN denotes E2f3a
T20655 27164-27176 NN denotes localization
T20656 27177-27180 MD denotes may
T20657 27181-27183 VB denotes be
T20653 27184-27193 VBN denotes regulated
T20658 27194-27196 IN denotes by
T20659 27197-27199 RB denotes as
T20660 27200-27203 RB denotes yet
T20661 27204-27211 JJ denotes unknown
T20663 27212-27230 JJ denotes post-translational
T20662 27231-27244 NNS denotes modifications
T20664 27244-27245 . denotes .
T20665 27245-29189 sentence denotes Figure 6 Subcellular Distribution of E2f3 Isoforms and Other Cell Cycle Proteins in the Developing Retina Nuclear and cytoplasmic extracts from an equivalent number of retinal cells from mice of the indicated genotypes and ages were analyzed by Western blotting to detect the indicated proteins. Lysate from E2f3a−/− mice was used as a control to confirm the location of E2f3a protein. C, cytoplasmic extracts; N, nuclear extracts. Figure 7 The E2f3a Isoform Drives the Differentiation Defect in Rb KO SACs (A) Schematic diagrams of the mouse WT, E2f3a−/−, and the Cre-recombined floxed E2f3 loci (indicated here as E2f3−/− for simplicity). E2f3a−/− mice lack most of E2f3 exon 1a and part of intron 1a (red dotted box). Arrows indicate PCR primers. Genotyping of an E2f3a+/− mouse is shown on the right. (B) RT-PCR detection of E2f3a and E2f3b mRNA in the retina. The sequences of primers are 1aF (5′-GCCTCTACACCACGCCACAAG-3′), 1bF (5′-CGGAAATGCCCTTACAGC-3′), and 4R (5′-CTCAGTCACTTCTTTGGACAG-3′). WT retina expresses both E2f3a and E2f3b mRNA. As expected, E2f3a−/− retina lacks E2f3a mRNA and still expresses E2f3b mRNA. E2f3−/− retina lacks full-length E2f3a and E2f3b mRNAs, and instead expresses a truncated mRNA lacking exon 3. (C) Real-time RT-PCR analysis of E2f genes in P8 retinas of the indicated genotypes. Error bars represent SD of measurements from three animals, and asterisks indicate a significant difference between WT and the indicated genotypes (*, p <0.05; **; p <0.01; ANOVA and Tukey HSD test). (D) Rescue of Rb KO SACs by E2f3a deletion. Horizontal retinal sections of the indicated genotypes and ages were stained for nuclei (DAPI, blue), M-phase (PH3, green), and the SAC marker Slc18a3 (red). E2f3a deletion does not suppress ectopic division, but rescues the SAC defect. Scale bars are 50 μm. M, molecular size marker. We also examined the distribution of other cell cycle regulators during retinal development.
T37145 27256-27267 JJ denotes Subcellular
T37146 27268-27280 NN denotes Distribution
T37147 27281-27283 IN denotes of
T37148 27284-27288 NN denotes E2f3
T37149 27289-27297 NNS denotes Isoforms
T37150 27298-27301 CC denotes and
T37151 27302-27307 JJ denotes Other
T37153 27308-27312 NN denotes Cell
T37154 27313-27318 NN denotes Cycle
T37152 27319-27327 NNS denotes Proteins
T37155 27328-27330 IN denotes in
T37156 27331-27334 DT denotes the
T37158 27335-27345 VBG denotes Developing
T37157 27346-27352 NN denotes Retina
T37159 27352-27542 sentence denotes Nuclear and cytoplasmic extracts from an equivalent number of retinal cells from mice of the indicated genotypes and ages were analyzed by Western blotting to detect the indicated proteins.
T37160 27353-27360 JJ denotes Nuclear
T37162 27361-27364 CC denotes and
T37163 27365-27376 JJ denotes cytoplasmic
T37161 27377-27385 NNS denotes extracts
T37165 27386-27390 IN denotes from
T37166 27391-27393 DT denotes an
T37168 27394-27404 JJ denotes equivalent
T37167 27405-27411 NN denotes number
T37169 27412-27414 IN denotes of
T37170 27415-27422 JJ denotes retinal
T37171 27423-27428 NNS denotes cells
T37172 27429-27433 IN denotes from
T37173 27434-27438 NNS denotes mice
T37174 27439-27441 IN denotes of
T37175 27442-27445 DT denotes the
T37177 27446-27455 VBN denotes indicated
T37176 27456-27465 NNS denotes genotypes
T37178 27466-27469 CC denotes and
T37179 27470-27474 NNS denotes ages
T37180 27475-27479 VBD denotes were
T37164 27480-27488 VBN denotes analyzed
T37181 27489-27491 IN denotes by
T37182 27492-27499 NNP denotes Western
T37183 27500-27508 NN denotes blotting
T37184 27509-27511 TO denotes to
T37185 27512-27518 VB denotes detect
T37186 27519-27522 DT denotes the
T37188 27523-27532 VBN denotes indicated
T37187 27533-27541 NN denotes proteins
T37189 27541-27542 . denotes .
T37190 27542-27632 sentence denotes Lysate from E2f3a−/− mice was used as a control to confirm the location of E2f3a protein.
T37191 27543-27549 NN denotes Lysate
T37193 27550-27554 IN denotes from
T37194 27555-27560 NN denotes E2f3a
T37196 27560-27561 SYM denotes −
T37197 27561-27562 HYPH denotes /
T37198 27562-27563 SYM denotes −
T37195 27564-27568 NNS denotes mice
T37199 27569-27572 VBD denotes was
T37192 27573-27577 VBN denotes used
T37200 27578-27580 IN denotes as
T37201 27581-27582 DT denotes a
T37202 27583-27590 NN denotes control
T37203 27591-27593 TO denotes to
T37204 27594-27601 VB denotes confirm
T37205 27602-27605 DT denotes the
T37206 27606-27614 NN denotes location
T37207 27615-27617 IN denotes of
T37208 27618-27623 NN denotes E2f3a
T37209 27624-27631 NN denotes protein
T37210 27631-27632 . denotes .
T37211 27632-27678 sentence denotes C, cytoplasmic extracts; N, nuclear extracts.
T37212 27633-27634 NN denotes C
T37213 27634-27636 , denotes ,
T37214 27636-27647 JJ denotes cytoplasmic
T37215 27648-27656 NNS denotes extracts
T37216 27656-27657 : denotes ;
T37217 27658-27659 NN denotes N
T37218 27659-27661 , denotes ,
T37219 27661-27668 JJ denotes nuclear
T37220 27669-27677 NNS denotes extracts
T37221 27677-27678 . denotes .
T37915 27689-27692 DT denotes The
T37917 27693-27698 NN denotes E2f3a
T37916 27699-27706 NN denotes Isoform
T37918 27707-27713 VBZ denotes Drives
T37919 27714-27717 DT denotes the
T37921 27718-27733 NN denotes Differentiation
T37920 27734-27740 NN denotes Defect
T37922 27741-27743 IN denotes in
T37923 27744-27746 NN denotes Rb
T37924 27747-27749 NN denotes KO
T37925 27750-27754 NNS denotes SACs
T37926 27754-27888 sentence denotes (A) Schematic diagrams of the mouse WT, E2f3a−/−, and the Cre-recombined floxed E2f3 loci (indicated here as E2f3−/− for simplicity).
T37927 27755-27756 -LRB- denotes (
T37928 27756-27757 LS denotes A
T37930 27757-27758 -RRB- denotes )
T37931 27759-27768 JJ denotes Schematic
T37929 27769-27777 NNS denotes diagrams
T37932 27778-27780 IN denotes of
T37933 27781-27784 DT denotes the
T37935 27785-27790 NN denotes mouse
T37934 27791-27793 NN denotes WT
T37936 27793-27795 , denotes ,
T37937 27795-27800 NN denotes E2f3a
T37938 27800-27801 SYM denotes −
T37939 27801-27802 HYPH denotes /
T37940 27802-27803 SYM denotes −
T37941 27803-27805 , denotes ,
T37942 27805-27808 CC denotes and
T37943 27809-27812 DT denotes the
T37945 27813-27816 NN denotes Cre
T37946 27816-27817 HYPH denotes -
T37947 27817-27827 VBN denotes recombined
T37948 27828-27834 VBN denotes floxed
T37949 27835-27839 NN denotes E2f3
T37944 27840-27844 NNS denotes loci
T37950 27845-27846 -LRB- denotes (
T37951 27846-27855 VBN denotes indicated
T37952 27856-27860 RB denotes here
T37953 27861-27863 IN denotes as
T37954 27864-27868 NN denotes E2f3
T37955 27868-27869 SYM denotes −
T37956 27869-27870 HYPH denotes /
T37957 27870-27871 SYM denotes −
T37958 27872-27875 IN denotes for
T37959 27876-27886 NN denotes simplicity
T37960 27886-27887 -RRB- denotes )
T37961 27887-27888 . denotes .
T37962 27888-27968 sentence denotes E2f3a−/− mice lack most of E2f3 exon 1a and part of intron 1a (red dotted box).
T37963 27889-27894 NN denotes E2f3a
T37965 27894-27895 SYM denotes −
T37966 27895-27896 HYPH denotes /
T37967 27896-27897 SYM denotes −
T37964 27898-27902 NNS denotes mice
T37968 27903-27907 VBP denotes lack
T37969 27908-27912 JJS denotes most
T37970 27913-27915 IN denotes of
T37971 27916-27920 NN denotes E2f3
T37973 27921-27925 NN denotes exon
T37972 27926-27928 NN denotes 1a
T37974 27929-27932 CC denotes and
T37975 27933-27937 NN denotes part
T37976 27938-27940 IN denotes of
T37977 27941-27947 NN denotes intron
T37978 27948-27950 NN denotes 1a
T37979 27951-27952 -LRB- denotes (
T37981 27952-27955 JJ denotes red
T37982 27956-27962 VBN denotes dotted
T37980 27963-27966 NN denotes box
T37983 27966-27967 -RRB- denotes )
T37984 27967-27968 . denotes .
T37985 27968-27997 sentence denotes Arrows indicate PCR primers.
T37986 27969-27975 NNS denotes Arrows
T37987 27976-27984 VBP denotes indicate
T37988 27985-27988 NN denotes PCR
T37989 27989-27996 NNS denotes primers
T37990 27996-27997 . denotes .
T37991 27997-28052 sentence denotes Genotyping of an E2f3a+/− mouse is shown on the right.
T37992 27998-28008 NN denotes Genotyping
T37994 28009-28011 IN denotes of
T37995 28012-28014 DT denotes an
T37997 28015-28020 NN denotes E2f3a
T37998 28020-28021 SYM denotes +
T37999 28021-28022 HYPH denotes /
T38000 28022-28023 SYM denotes −
T37996 28024-28029 NN denotes mouse
T38001 28030-28032 VBZ denotes is
T37993 28033-28038 VBN denotes shown
T38002 28039-28041 IN denotes on
T38003 28042-28045 DT denotes the
T38004 28046-28051 NN denotes right
T38005 28051-28052 . denotes .
T38006 28052-28112 sentence denotes (B) RT-PCR detection of E2f3a and E2f3b mRNA in the retina.
T38007 28053-28054 -LRB- denotes (
T38008 28054-28055 LS denotes B
T38010 28055-28056 -RRB- denotes )
T38011 28057-28059 NN denotes RT
T38013 28059-28060 HYPH denotes -
T38012 28060-28063 NN denotes PCR
T38009 28064-28073 NN denotes detection
T38014 28074-28076 IN denotes of
T38015 28077-28082 NN denotes E2f3a
T38017 28083-28086 CC denotes and
T38018 28087-28092 NN denotes E2f3b
T38016 28093-28097 NN denotes mRNA
T38019 28098-28100 IN denotes in
T38020 28101-28104 DT denotes the
T38021 28105-28111 NN denotes retina
T38022 28111-28112 . denotes .
T38023 28112-28246 sentence denotes The sequences of primers are 1aF (5′-GCCTCTACACCACGCCACAAG-3′), 1bF (5′-CGGAAATGCCCTTACAGC-3′), and 4R (5′-CTCAGTCACTTCTTTGGACAG-3′).
T38024 28113-28116 DT denotes The
T38025 28117-28126 NNS denotes sequences
T38027 28127-28129 IN denotes of
T38028 28130-28137 NNS denotes primers
T38026 28138-28141 VBP denotes are
T38029 28142-28145 NN denotes 1aF
T38030 28146-28147 -LRB- denotes (
T38031 28147-28148 CD denotes 5
T38033 28148-28149 SYM denotes ′
T38034 28149-28150 HYPH denotes -
T38032 28150-28171 NN denotes GCCTCTACACCACGCCACAAG
T38035 28171-28172 HYPH denotes -
T38036 28172-28173 CD denotes 3
T38037 28173-28174 SYM denotes ′
T38038 28174-28175 -RRB- denotes )
T38039 28175-28177 , denotes ,
T38040 28177-28180 NN denotes 1bF
T38041 28181-28182 -LRB- denotes (
T38042 28182-28183 CD denotes 5
T38044 28183-28184 SYM denotes ′
T38045 28184-28185 HYPH denotes -
T38043 28185-28203 NN denotes CGGAAATGCCCTTACAGC
T38046 28203-28204 HYPH denotes -
T38047 28204-28205 CD denotes 3
T38048 28205-28206 SYM denotes ′
T38049 28206-28207 -RRB- denotes )
T38050 28207-28209 , denotes ,
T38051 28209-28212 CC denotes and
T38052 28213-28215 NN denotes 4R
T38053 28216-28217 -LRB- denotes (
T38054 28217-28218 CD denotes 5
T38056 28218-28219 SYM denotes ′
T38057 28219-28220 HYPH denotes -
T38055 28220-28241 NN denotes CTCAGTCACTTCTTTGGACAG
T38058 28241-28242 HYPH denotes -
T38059 28242-28243 CD denotes 3
T38060 28243-28244 SYM denotes ′
T38061 28244-28245 -RRB- denotes )
T38062 28245-28246 . denotes .
T38063 28246-28293 sentence denotes WT retina expresses both E2f3a and E2f3b mRNA.
T38064 28247-28249 NN denotes WT
T38065 28250-28256 NN denotes retina
T38066 28257-28266 VBZ denotes expresses
T38067 28267-28271 CC denotes both
T38068 28272-28277 NN denotes E2f3a
T38070 28278-28281 CC denotes and
T38071 28282-28287 NN denotes E2f3b
T38069 28288-28292 NN denotes mRNA
T38072 28292-28293 . denotes .
T38073 28293-28371 sentence denotes As expected, E2f3a−/− retina lacks E2f3a mRNA and still expresses E2f3b mRNA.
T38074 28294-28296 IN denotes As
T38075 28297-28305 VBN denotes expected
T38077 28305-28307 , denotes ,
T38078 28307-28312 NN denotes E2f3a
T38080 28312-28313 SYM denotes −
T38081 28313-28314 HYPH denotes /
T38082 28314-28315 SYM denotes −
T38079 28316-28322 NN denotes retina
T38076 28323-28328 VBZ denotes lacks
T38083 28329-28334 NN denotes E2f3a
T38084 28335-28339 NN denotes mRNA
T38085 28340-28343 CC denotes and
T38086 28344-28349 RB denotes still
T38087 28350-28359 VBZ denotes expresses
T38088 28360-28365 NN denotes E2f3b
T38089 28366-28370 NN denotes mRNA
T38090 28370-28371 . denotes .
T38091 28371-28482 sentence denotes E2f3−/− retina lacks full-length E2f3a and E2f3b mRNAs, and instead expresses a truncated mRNA lacking exon 3.
T38092 28372-28376 NN denotes E2f3
T38094 28376-28377 SYM denotes −
T38095 28377-28378 HYPH denotes /
T38096 28378-28379 SYM denotes −
T38093 28380-28386 NN denotes retina
T38097 28387-28392 VBZ denotes lacks
T38098 28393-28397 JJ denotes full
T38100 28397-28398 HYPH denotes -
T38099 28398-28404 NN denotes length
T38102 28405-28410 NN denotes E2f3a
T38103 28411-28414 CC denotes and
T38104 28415-28420 NN denotes E2f3b
T38101 28421-28426 NNS denotes mRNAs
T38105 28426-28428 , denotes ,
T38106 28428-28431 CC denotes and
T38107 28432-28439 RB denotes instead
T38108 28440-28449 VBZ denotes expresses
T38109 28450-28451 DT denotes a
T38111 28452-28461 VBN denotes truncated
T38110 28462-28466 NN denotes mRNA
T38112 28467-28474 VBG denotes lacking
T38113 28475-28479 NN denotes exon
T38114 28480-28481 CD denotes 3
T38115 28481-28482 . denotes .
T38116 28482-28567 sentence denotes (C) Real-time RT-PCR analysis of E2f genes in P8 retinas of the indicated genotypes.
T38117 28483-28484 -LRB- denotes (
T38118 28484-28485 LS denotes C
T38120 28485-28486 -RRB- denotes )
T38121 28487-28491 JJ denotes Real
T38123 28491-28492 HYPH denotes -
T38122 28492-28496 NN denotes time
T38124 28497-28499 NN denotes RT
T38126 28499-28500 HYPH denotes -
T38125 28500-28503 NN denotes PCR
T38119 28504-28512 NN denotes analysis
T38127 28513-28515 IN denotes of
T38128 28516-28519 NN denotes E2f
T38129 28520-28525 NNS denotes genes
T38130 28526-28528 IN denotes in
T38131 28529-28531 NN denotes P8
T38132 28532-28539 NNS denotes retinas
T38133 28540-28542 IN denotes of
T38134 28543-28546 DT denotes the
T38136 28547-28556 VBN denotes indicated
T38135 28557-28566 NNS denotes genotypes
T38137 28566-28567 . denotes .
T38138 28567-28767 sentence denotes Error bars represent SD of measurements from three animals, and asterisks indicate a significant difference between WT and the indicated genotypes (*, p <0.05; **; p <0.01; ANOVA and Tukey HSD test).
T38139 28568-28573 NN denotes Error
T38140 28574-28578 NNS denotes bars
T38141 28579-28588 VBP denotes represent
T38142 28589-28591 NN denotes SD
T38143 28592-28594 IN denotes of
T38144 28595-28607 NNS denotes measurements
T38145 28608-28612 IN denotes from
T38146 28613-28618 CD denotes three
T38147 28619-28626 NNS denotes animals
T38148 28626-28628 , denotes ,
T38149 28628-28631 CC denotes and
T38150 28632-28641 NNS denotes asterisks
T38151 28642-28650 VBP denotes indicate
T38152 28651-28652 DT denotes a
T38154 28653-28664 JJ denotes significant
T38153 28665-28675 NN denotes difference
T38155 28676-28683 IN denotes between
T38156 28684-28686 NN denotes WT
T38157 28687-28690 CC denotes and
T38158 28691-28694 DT denotes the
T38159 28695-28704 VBN denotes indicated
T38160 28705-28714 NNS denotes genotypes
T38161 28715-28716 -LRB- denotes (
T38163 28716-28717 SYM denotes *
T38165 28717-28719 , denotes ,
T38166 28719-28720 NN denotes p
T38167 28721-28722 SYM denotes <
T38164 28722-28726 CD denotes 0.05
T38168 28726-28727 : denotes ;
T38169 28728-28730 SYM denotes **
T38171 28730-28731 : denotes ;
T38172 28732-28733 NN denotes p
T38173 28734-28735 SYM denotes <
T38170 28735-28739 CD denotes 0.01
T38174 28739-28740 : denotes ;
T38175 28741-28746 NN denotes ANOVA
T38176 28747-28750 CC denotes and
T38177 28751-28756 NNP denotes Tukey
T38178 28757-28760 NN denotes HSD
T38162 28761-28765 NN denotes test
T38179 28765-28766 -RRB- denotes )
T38180 28766-28767 . denotes .
T38181 28767-28811 sentence denotes (D) Rescue of Rb KO SACs by E2f3a deletion.
T38182 28768-28769 -LRB- denotes (
T38183 28769-28770 LS denotes D
T38185 28770-28771 -RRB- denotes )
T38184 28772-28778 NN denotes Rescue
T38186 28779-28781 IN denotes of
T38187 28782-28784 NN denotes Rb
T38188 28785-28787 NN denotes KO
T38189 28788-28792 NNS denotes SACs
T38190 28793-28795 IN denotes by
T38191 28796-28801 NN denotes E2f3a
T38192 28802-28810 NN denotes deletion
T38193 28810-28811 . denotes .
T38194 28811-28969 sentence denotes Horizontal retinal sections of the indicated genotypes and ages were stained for nuclei (DAPI, blue), M-phase (PH3, green), and the SAC marker Slc18a3 (red).
T38195 28812-28822 JJ denotes Horizontal
T38197 28823-28830 JJ denotes retinal
T38196 28831-28839 NNS denotes sections
T38199 28840-28842 IN denotes of
T38200 28843-28846 DT denotes the
T38202 28847-28856 VBN denotes indicated
T38201 28857-28866 NNS denotes genotypes
T38203 28867-28870 CC denotes and
T38204 28871-28875 NNS denotes ages
T38205 28876-28880 VBD denotes were
T38198 28881-28888 VBN denotes stained
T38206 28889-28892 IN denotes for
T38207 28893-28899 NNS denotes nuclei
T38208 28900-28901 -LRB- denotes (
T38210 28901-28905 NN denotes DAPI
T38211 28905-28907 , denotes ,
T38209 28907-28911 JJ denotes blue
T38212 28911-28912 -RRB- denotes )
T38213 28912-28914 , denotes ,
T38214 28914-28915 NN denotes M
T38216 28915-28916 HYPH denotes -
T38215 28916-28921 NN denotes phase
T38217 28922-28923 -LRB- denotes (
T38219 28923-28926 NN denotes PH3
T38220 28926-28928 , denotes ,
T38218 28928-28933 JJ denotes green
T38221 28933-28934 -RRB- denotes )
T38222 28934-28936 , denotes ,
T38223 28936-28939 CC denotes and
T38224 28940-28943 DT denotes the
T38226 28944-28947 NN denotes SAC
T38225 28948-28954 NN denotes marker
T38227 28955-28962 NN denotes Slc18a3
T38228 28963-28964 -LRB- denotes (
T38229 28964-28967 JJ denotes red
T38230 28967-28968 -RRB- denotes )
T38231 28968-28969 . denotes .
T38232 28969-29048 sentence denotes E2f3a deletion does not suppress ectopic division, but rescues the SAC defect.
T38233 28970-28975 NN denotes E2f3a
T38234 28976-28984 NN denotes deletion
T38236 28985-28989 VBZ denotes does
T38237 28990-28993 RB denotes not
T38235 28994-29002 VB denotes suppress
T38238 29003-29010 JJ denotes ectopic
T38239 29011-29019 NN denotes division
T38240 29019-29021 , denotes ,
T38241 29021-29024 CC denotes but
T38242 29025-29032 VBZ denotes rescues
T38243 29033-29036 DT denotes the
T38245 29037-29040 NN denotes SAC
T38244 29041-29047 NN denotes defect
T38246 29047-29048 . denotes .
T38247 29048-29070 sentence denotes Scale bars are 50 μm.
T38248 29049-29054 NN denotes Scale
T38249 29055-29059 NNS denotes bars
T38250 29060-29063 VBP denotes are
T38251 29064-29066 CD denotes 50
T38252 29067-29069 NN denotes μm
T38253 29069-29070 . denotes .
T38254 29070-29096 sentence denotes M, molecular size marker.
T38255 29071-29072 NN denotes M
T38256 29072-29074 , denotes ,
T38257 29074-29083 JJ denotes molecular
T38258 29084-29088 NN denotes size
T38259 29089-29095 NN denotes marker
T38260 29095-29096 . denotes .
T20666 29097-29099 PRP denotes We
T20668 29100-29104 RB denotes also
T20667 29105-29113 VBD denotes examined
T20669 29114-29117 DT denotes the
T20670 29118-29130 NN denotes distribution
T20671 29131-29133 IN denotes of
T20672 29134-29139 JJ denotes other
T20674 29140-29144 NN denotes cell
T20675 29145-29150 NN denotes cycle
T20673 29151-29161 NNS denotes regulators
T20676 29162-29168 IN denotes during
T20677 29169-29176 JJ denotes retinal
T20678 29177-29188 NN denotes development
T20679 29188-29189 . denotes .
T20680 29189-29346 sentence denotes Like E2f3a, Rb was present in both the WT cytoplasm and nucleus at P0, but at P18, when the levels of Rb had increased, it was primarily nuclear (Figure 6).
T20681 29190-29194 IN denotes Like
T20683 29195-29200 NN denotes E2f3a
T20684 29200-29202 , denotes ,
T20685 29202-29204 NN denotes Rb
T20682 29205-29208 VBD denotes was
T20686 29209-29216 JJ denotes present
T20687 29217-29219 IN denotes in
T20688 29220-29224 CC denotes both
T20690 29225-29228 DT denotes the
T20691 29229-29231 NN denotes WT
T20689 29232-29241 NN denotes cytoplasm
T20692 29242-29245 CC denotes and
T20693 29246-29253 NN denotes nucleus
T20694 29254-29256 IN denotes at
T20695 29257-29259 NN denotes P0
T20696 29259-29261 , denotes ,
T20697 29261-29264 CC denotes but
T20698 29265-29267 IN denotes at
T20700 29268-29271 NN denotes P18
T20701 29271-29273 , denotes ,
T20702 29273-29277 WRB denotes when
T20704 29278-29281 DT denotes the
T20705 29282-29288 NNS denotes levels
T20706 29289-29291 IN denotes of
T20707 29292-29294 NN denotes Rb
T20708 29295-29298 VBD denotes had
T20703 29299-29308 VBN denotes increased
T20709 29308-29310 , denotes ,
T20710 29310-29312 PRP denotes it
T20699 29313-29316 VBD denotes was
T20711 29317-29326 RB denotes primarily
T20712 29327-29334 JJ denotes nuclear
T20713 29335-29336 -LRB- denotes (
T20714 29336-29342 NN denotes Figure
T20715 29343-29344 CD denotes 6
T20716 29344-29345 -RRB- denotes )
T20717 29345-29346 . denotes .
T20718 29346-29531 sentence denotes A very faint cytoplasmic Rb signal was evident at P18, which is consistent with Rb staining of SAC processes (Figure 5B), and with the very small proportion of SACs in the retina [38].
T20719 29347-29348 DT denotes A
T20721 29349-29353 RB denotes very
T20722 29354-29359 JJ denotes faint
T20723 29360-29371 JJ denotes cytoplasmic
T20724 29372-29374 NN denotes Rb
T20720 29375-29381 NN denotes signal
T20725 29382-29385 VBD denotes was
T20726 29386-29393 JJ denotes evident
T20727 29394-29396 IN denotes at
T20728 29397-29400 NN denotes P18
T20729 29400-29402 , denotes ,
T20730 29402-29407 WDT denotes which
T20731 29408-29410 VBZ denotes is
T20732 29411-29421 JJ denotes consistent
T20733 29422-29426 IN denotes with
T20734 29427-29429 NN denotes Rb
T20735 29430-29438 NN denotes staining
T20736 29439-29441 IN denotes of
T20737 29442-29445 NN denotes SAC
T20738 29446-29455 NNS denotes processes
T20739 29456-29457 -LRB- denotes (
T20741 29457-29463 NN denotes Figure
T20740 29464-29466 NN denotes 5B
T20742 29466-29467 -RRB- denotes )
T20743 29467-29469 , denotes ,
T20744 29469-29472 CC denotes and
T20745 29473-29477 IN denotes with
T20746 29478-29481 DT denotes the
T20748 29482-29486 RB denotes very
T20749 29487-29492 JJ denotes small
T20747 29493-29503 NN denotes proportion
T20750 29504-29506 IN denotes of
T20751 29507-29511 NNS denotes SACs
T20752 29512-29514 IN denotes in
T20753 29515-29518 DT denotes the
T20754 29519-29525 NN denotes retina
T20755 29526-29527 -LRB- denotes [
T20756 29527-29529 CD denotes 38
T20757 29529-29530 -RRB- denotes ]
T20758 29530-29531 . denotes .
T20759 29531-29679 sentence denotes E2f1 was also detected in both nuclear and cytoplasmic fractions, although unlike E2f3a it was predominantly nuclear both at P0 and P18 (Figure 6).
T20760 29532-29536 NN denotes E2f1
T20762 29537-29540 VBD denotes was
T20763 29541-29545 RB denotes also
T20761 29546-29554 VBN denotes detected
T20764 29555-29557 IN denotes in
T20765 29558-29562 CC denotes both
T20766 29563-29570 JJ denotes nuclear
T20768 29571-29574 CC denotes and
T20769 29575-29586 JJ denotes cytoplasmic
T20767 29587-29596 NNS denotes fractions
T20770 29596-29598 , denotes ,
T20771 29598-29606 IN denotes although
T20773 29607-29613 IN denotes unlike
T20774 29614-29619 NN denotes E2f3a
T20775 29620-29622 PRP denotes it
T20772 29623-29626 VBD denotes was
T20776 29627-29640 RB denotes predominantly
T20777 29641-29648 JJ denotes nuclear
T20778 29649-29653 CC denotes both
T20779 29654-29656 IN denotes at
T20780 29657-29659 NN denotes P0
T20781 29660-29663 CC denotes and
T20782 29664-29667 NN denotes P18
T20783 29668-29669 -LRB- denotes (
T20784 29669-29675 NN denotes Figure
T20785 29676-29677 CD denotes 6
T20786 29677-29678 -RRB- denotes )
T20787 29678-29679 . denotes .
T20788 29679-29904 sentence denotes The E2f dimerization partner, Tfdp1, which lacks a nuclear localization signal [54], was primarily cytoplasmic at both P0 and P18, and the Cdk inhibitors Cdkn1a and Cdkn1b showed a similar pattern of distribution (Figure 6).
T20789 29680-29683 DT denotes The
T20791 29684-29687 NN denotes E2f
T20792 29688-29700 NN denotes dimerization
T20790 29701-29708 NN denotes partner
T20794 29708-29710 , denotes ,
T20795 29710-29715 NN denotes Tfdp1
T20796 29715-29717 , denotes ,
T20797 29717-29722 WDT denotes which
T20798 29723-29728 VBZ denotes lacks
T20799 29729-29730 DT denotes a
T20801 29731-29738 JJ denotes nuclear
T20802 29739-29751 NN denotes localization
T20800 29752-29758 NN denotes signal
T20803 29759-29760 -LRB- denotes [
T20804 29760-29762 CD denotes 54
T20805 29762-29763 -RRB- denotes ]
T20806 29763-29765 , denotes ,
T20793 29765-29768 VBD denotes was
T20807 29769-29778 RB denotes primarily
T20808 29779-29790 JJ denotes cytoplasmic
T20809 29791-29793 IN denotes at
T20810 29794-29798 CC denotes both
T20811 29799-29801 NN denotes P0
T20812 29802-29805 CC denotes and
T20813 29806-29809 NN denotes P18
T20814 29809-29811 , denotes ,
T20815 29811-29814 CC denotes and
T20816 29815-29818 DT denotes the
T20818 29819-29822 NN denotes Cdk
T20817 29823-29833 NNS denotes inhibitors
T20820 29834-29840 NN denotes Cdkn1a
T20821 29841-29844 CC denotes and
T20822 29845-29851 NN denotes Cdkn1b
T20819 29852-29858 VBD denotes showed
T20823 29859-29860 DT denotes a
T20825 29861-29868 JJ denotes similar
T20824 29869-29876 NN denotes pattern
T20826 29877-29879 IN denotes of
T20827 29880-29892 NN denotes distribution
T20828 29893-29894 -LRB- denotes (
T20829 29894-29900 NN denotes Figure
T20830 29901-29902 CD denotes 6
T20831 29902-29903 -RRB- denotes )
T20832 29903-29904 . denotes .
T20833 29904-30056 sentence denotes Thus, among the cell cycle regulators we examined, most showed bivalent distribution, and E2f3b was unusual in its solely nuclear compartmentalization.
T20834 29905-29909 RB denotes Thus
T20836 29909-29911 , denotes ,
T20837 29911-29916 IN denotes among
T20838 29917-29920 DT denotes the
T20840 29921-29925 NN denotes cell
T20841 29926-29931 NN denotes cycle
T20839 29932-29942 NNS denotes regulators
T20842 29943-29945 PRP denotes we
T20843 29946-29954 VBD denotes examined
T20844 29954-29956 , denotes ,
T20845 29956-29960 JJS denotes most
T20835 29961-29967 VBD denotes showed
T20846 29968-29976 JJ denotes bivalent
T20847 29977-29989 NN denotes distribution
T20848 29989-29991 , denotes ,
T20849 29991-29994 CC denotes and
T20850 29995-30000 NN denotes E2f3b
T20851 30001-30004 VBD denotes was
T20852 30005-30012 JJ denotes unusual
T20853 30013-30015 IN denotes in
T20854 30016-30019 PRP$ denotes its
T20856 30020-30026 RB denotes solely
T20857 30027-30034 JJ denotes nuclear
T20855 30035-30055 NN denotes compartmentalization
T20858 30055-30056 . denotes .
T21488 30058-30060 NN denotes Rb
T21489 30061-30070 VBZ denotes Regulates
T21490 30071-30074 NN denotes SAC
T21491 30075-30090 NN denotes Differentiation
T21492 30091-30098 IN denotes through
T21493 30099-30104 NN denotes E2f3a
T21494 30104-30225 sentence denotes To test which E2f3 isoform is responsible for aberrant Rb KO SAC differentiation we exploited E2f3a−/− mice (Figure 7A).
T21495 30105-30107 TO denotes To
T21496 30108-30112 VB denotes test
T21498 30113-30118 WDT denotes which
T21500 30119-30123 NN denotes E2f3
T21499 30124-30131 NN denotes isoform
T21501 30132-30134 VBZ denotes is
T21502 30135-30146 JJ denotes responsible
T21503 30147-30150 IN denotes for
T21504 30151-30159 JJ denotes aberrant
T21506 30160-30162 NN denotes Rb
T21507 30163-30165 NN denotes KO
T21508 30166-30169 NN denotes SAC
T21505 30170-30185 NN denotes differentiation
T21509 30186-30188 PRP denotes we
T21497 30189-30198 VBD denotes exploited
T21510 30199-30204 NN denotes E2f3a
T21512 30204-30205 SYM denotes −
T21513 30205-30206 HYPH denotes /
T21514 30206-30207 SYM denotes −
T21511 30208-30212 NNS denotes mice
T21515 30213-30214 -LRB- denotes (
T21517 30214-30220 NN denotes Figure
T21516 30221-30223 NN denotes 7A
T21518 30223-30224 -RRB- denotes )
T21519 30224-30225 . denotes .
T21520 30225-30328 sentence denotes The genotyping strategy outlined in Figure 7A was used to distinguish the E2f3a, WT, and null alleles.
T21521 30226-30229 DT denotes The
T21523 30230-30240 NN denotes genotyping
T21522 30241-30249 NN denotes strategy
T21525 30250-30258 VBN denotes outlined
T21526 30259-30261 IN denotes in
T21527 30262-30268 NN denotes Figure
T21528 30269-30271 NN denotes 7A
T21529 30272-30275 VBD denotes was
T21524 30276-30280 VBN denotes used
T21530 30281-30283 TO denotes to
T21531 30284-30295 VB denotes distinguish
T21532 30296-30299 DT denotes the
T21534 30300-30305 NN denotes E2f3a
T21535 30305-30307 , denotes ,
T21536 30307-30309 NN denotes WT
T21537 30309-30311 , denotes ,
T21538 30311-30314 CC denotes and
T21539 30315-30319 JJ denotes null
T21533 30320-30327 NNS denotes alleles
T21540 30327-30328 . denotes .
T21541 30328-30525 sentence denotes Reverse transcriptase PCR (RT-PCR) confirmed the presence of both E2f3a and E2f3b RNA species in the developing WT retina, and the specific absence of E2f3a RNA in the E2f3a−/− retina (Figure 7B).
T21542 30329-30336 JJ denotes Reverse
T21544 30337-30350 NN denotes transcriptase
T21543 30351-30354 NN denotes PCR
T21546 30355-30356 -LRB- denotes (
T21547 30356-30358 NN denotes RT
T21549 30358-30359 HYPH denotes -
T21548 30359-30362 NN denotes PCR
T21550 30362-30363 -RRB- denotes )
T21545 30364-30373 VBD denotes confirmed
T21551 30374-30377 DT denotes the
T21552 30378-30386 NN denotes presence
T21553 30387-30389 IN denotes of
T21554 30390-30394 CC denotes both
T21555 30395-30400 NN denotes E2f3a
T21557 30401-30404 CC denotes and
T21558 30405-30410 NN denotes E2f3b
T21559 30411-30414 NN denotes RNA
T21556 30415-30422 NNS denotes species
T21560 30423-30425 IN denotes in
T21561 30426-30429 DT denotes the
T21563 30430-30440 VBG denotes developing
T21564 30441-30443 NN denotes WT
T21562 30444-30450 NN denotes retina
T21565 30450-30452 , denotes ,
T21566 30452-30455 CC denotes and
T21567 30456-30459 DT denotes the
T21569 30460-30468 JJ denotes specific
T21568 30469-30476 NN denotes absence
T21570 30477-30479 IN denotes of
T21571 30480-30485 NN denotes E2f3a
T21572 30486-30489 NN denotes RNA
T21573 30490-30492 IN denotes in
T21574 30493-30496 DT denotes the
T21576 30497-30502 NN denotes E2f3a
T21577 30502-30503 SYM denotes −
T21578 30503-30504 HYPH denotes /
T21579 30504-30505 SYM denotes −
T21575 30506-30512 NN denotes retina
T21580 30513-30514 -LRB- denotes (
T21582 30514-30520 NN denotes Figure
T21581 30521-30523 NN denotes 7B
T21583 30523-30524 -RRB- denotes )
T21584 30524-30525 . denotes .
T21585 30525-30597 sentence denotes E2f3a protein was absent in E2f3a−/− retinal lysate (Figures 6 and S8).
T21586 30526-30531 NN denotes E2f3a
T21587 30532-30539 NN denotes protein
T21588 30540-30543 VBD denotes was
T21589 30544-30550 JJ denotes absent
T21590 30551-30553 IN denotes in
T21591 30554-30559 NN denotes E2f3a
T21593 30559-30560 SYM denotes −
T21594 30560-30561 HYPH denotes /
T21595 30561-30562 SYM denotes −
T21596 30563-30570 JJ denotes retinal
T21592 30571-30577 NN denotes lysate
T21597 30578-30579 -LRB- denotes (
T21599 30579-30586 NNS denotes Figures
T21600 30587-30588 CD denotes 6
T21601 30589-30592 CC denotes and
T21598 30593-30595 NN denotes S8
T21602 30595-30596 -RRB- denotes )
T21603 30596-30597 . denotes .
T21604 30597-30818 sentence denotes Importantly, the levels of E2f3b message were similar in the Rb KO and Rb/E2f3a DKO retina, ruling out the possibility that any effects of E2f3a deletion we might observe were due to down-regulation of E2f3b (Figure 7C).
T21605 30598-30609 RB denotes Importantly
T21607 30609-30611 , denotes ,
T21608 30611-30614 DT denotes the
T21609 30615-30621 NNS denotes levels
T21610 30622-30624 IN denotes of
T21611 30625-30630 NN denotes E2f3b
T21612 30631-30638 NN denotes message
T21606 30639-30643 VBD denotes were
T21613 30644-30651 JJ denotes similar
T21614 30652-30654 IN denotes in
T21615 30655-30658 DT denotes the
T21617 30659-30661 NN denotes Rb
T21618 30662-30664 NN denotes KO
T21619 30665-30668 CC denotes and
T21620 30669-30671 NN denotes Rb
T21622 30671-30672 HYPH denotes /
T21621 30672-30677 NN denotes E2f3a
T21623 30678-30681 NN denotes DKO
T21616 30682-30688 NN denotes retina
T21624 30688-30690 , denotes ,
T21625 30690-30696 VBG denotes ruling
T21626 30697-30700 RP denotes out
T21627 30701-30704 DT denotes the
T21628 30705-30716 NN denotes possibility
T21629 30717-30721 IN denotes that
T21631 30722-30725 DT denotes any
T21632 30726-30733 NNS denotes effects
T21633 30734-30736 IN denotes of
T21634 30737-30742 NN denotes E2f3a
T21635 30743-30751 NN denotes deletion
T21636 30752-30754 PRP denotes we
T21638 30755-30760 MD denotes might
T21637 30761-30768 VB denotes observe
T21630 30769-30773 VBD denotes were
T21639 30774-30777 IN denotes due
T21640 30778-30780 IN denotes to
T21641 30781-30785 JJ denotes down
T21643 30785-30786 HYPH denotes -
T21642 30786-30796 NN denotes regulation
T21644 30797-30799 IN denotes of
T21645 30800-30805 NN denotes E2f3b
T21646 30806-30807 -LRB- denotes (
T21648 30807-30813 NN denotes Figure
T21647 30814-30816 NN denotes 7C
T21649 30816-30817 -RRB- denotes )
T21650 30817-30818 . denotes .
T21651 30818-30973 sentence denotes Also, the levels of other E2fs were the same in the Rb KO, Rb/E2f3 DKO, and Rb/E2f3a DKO retina, ruling out any cross-regulatory effects (Figure 7C) [55].
T21652 30819-30823 RB denotes Also
T21654 30823-30825 , denotes ,
T21655 30825-30828 DT denotes the
T21656 30829-30835 NNS denotes levels
T21657 30836-30838 IN denotes of
T21658 30839-30844 JJ denotes other
T21659 30845-30849 NNS denotes E2fs
T21653 30850-30854 VBD denotes were
T21660 30855-30858 DT denotes the
T21661 30859-30863 JJ denotes same
T21662 30864-30866 IN denotes in
T21663 30867-30870 DT denotes the
T21665 30871-30873 NN denotes Rb
T21666 30874-30876 NN denotes KO
T21667 30876-30878 , denotes ,
T21668 30878-30880 NN denotes Rb
T21670 30880-30881 HYPH denotes /
T21669 30881-30885 NN denotes E2f3
T21671 30886-30889 NN denotes DKO
T21672 30889-30891 , denotes ,
T21673 30891-30894 CC denotes and
T21674 30895-30897 NN denotes Rb
T21676 30897-30898 HYPH denotes /
T21675 30898-30903 NN denotes E2f3a
T21677 30904-30907 NN denotes DKO
T21664 30908-30914 NN denotes retina
T21678 30914-30916 , denotes ,
T21679 30916-30922 VBG denotes ruling
T21680 30923-30926 RP denotes out
T21681 30927-30930 DT denotes any
T21683 30931-30936 RB denotes cross
T21685 30936-30937 HYPH denotes -
T21684 30937-30947 JJ denotes regulatory
T21682 30948-30955 NNS denotes effects
T21686 30956-30957 -LRB- denotes (
T21688 30957-30963 NN denotes Figure
T21687 30964-30966 NN denotes 7C
T21689 30966-30967 -RRB- denotes )
T21690 30968-30969 -LRB- denotes [
T21691 30969-30971 CD denotes 55
T21692 30971-30972 -RRB- denotes ]
T21693 30972-30973 . denotes .
T21694 30973-31258 sentence denotes E2f3a can trigger cell cycle induction, but because SAC defects are not linked to cell cycle perturbation (Figures 3A and 4), and in view of the predominant association between E2f3b and Rb in quiescent cells [16,19], we suspected that E2f3b may perturb differentiation in Rb KO SACs.
T21695 30974-30979 NN denotes E2f3a
T21697 30980-30983 MD denotes can
T21696 30984-30991 VB denotes trigger
T21698 30992-30996 NN denotes cell
T21699 30997-31002 NN denotes cycle
T21700 31003-31012 NN denotes induction
T21701 31012-31014 , denotes ,
T21702 31014-31017 CC denotes but
T21703 31018-31025 IN denotes because
T21705 31026-31029 NN denotes SAC
T21706 31030-31037 NNS denotes defects
T21707 31038-31041 VBP denotes are
T21708 31042-31045 RB denotes not
T21704 31046-31052 VBN denotes linked
T21710 31053-31055 IN denotes to
T21711 31056-31060 NN denotes cell
T21712 31061-31066 NN denotes cycle
T21713 31067-31079 NN denotes perturbation
T21714 31080-31081 -LRB- denotes (
T21716 31081-31088 NNS denotes Figures
T21715 31089-31091 NN denotes 3A
T21717 31092-31095 CC denotes and
T21718 31096-31097 CD denotes 4
T21719 31097-31098 -RRB- denotes )
T21720 31098-31100 , denotes ,
T21721 31100-31103 CC denotes and
T21722 31104-31106 IN denotes in
T21723 31107-31111 NN denotes view
T21724 31112-31114 IN denotes of
T21725 31115-31118 DT denotes the
T21727 31119-31130 JJ denotes predominant
T21726 31131-31142 NN denotes association
T21728 31143-31150 IN denotes between
T21729 31151-31156 NN denotes E2f3b
T21730 31157-31160 CC denotes and
T21731 31161-31163 NN denotes Rb
T21732 31164-31166 IN denotes in
T21733 31167-31176 JJ denotes quiescent
T21734 31177-31182 NNS denotes cells
T21735 31183-31184 -LRB- denotes [
T21737 31184-31186 CD denotes 16
T21738 31186-31187 , denotes ,
T21736 31187-31189 CD denotes 19
T21739 31189-31190 -RRB- denotes ]
T21740 31190-31192 , denotes ,
T21741 31192-31194 PRP denotes we
T21709 31195-31204 VBD denotes suspected
T21742 31205-31209 IN denotes that
T21744 31210-31215 NN denotes E2f3b
T21745 31216-31219 MD denotes may
T21743 31220-31227 VB denotes perturb
T21746 31228-31243 NN denotes differentiation
T21747 31244-31246 IN denotes in
T21748 31247-31249 NN denotes Rb
T21749 31250-31252 NN denotes KO
T21750 31253-31257 NNS denotes SACs
T21751 31257-31258 . denotes .
T21752 31258-31341 sentence denotes Unexpectedly, however, E2f3a deletion suppressed the Rb KO SAC defect (Figure 7D).
T21753 31259-31271 RB denotes Unexpectedly
T21755 31271-31273 , denotes ,
T21756 31273-31280 RB denotes however
T21757 31280-31282 , denotes ,
T21758 31282-31287 NN denotes E2f3a
T21759 31288-31296 NN denotes deletion
T21754 31297-31307 VBD denotes suppressed
T21760 31308-31311 DT denotes the
T21762 31312-31314 NN denotes Rb
T21763 31315-31317 NN denotes KO
T21764 31318-31321 NN denotes SAC
T21761 31322-31328 NN denotes defect
T21765 31329-31330 -LRB- denotes (
T21767 31330-31336 NN denotes Figure
T21766 31337-31339 NN denotes 7D
T21768 31339-31340 -RRB- denotes )
T21769 31340-31341 . denotes .
T21770 31341-31471 sentence denotes Thus, separate from its role in cell cycle control, Rb regulation of E2f3a is critical to ensure proper neuronal differentiation.
T21771 31342-31346 RB denotes Thus
T21773 31346-31348 , denotes ,
T21774 31348-31356 JJ denotes separate
T21775 31357-31361 IN denotes from
T21776 31362-31365 PRP$ denotes its
T21777 31366-31370 NN denotes role
T21778 31371-31373 IN denotes in
T21779 31374-31378 NN denotes cell
T21780 31379-31384 NN denotes cycle
T21781 31385-31392 NN denotes control
T21782 31392-31394 , denotes ,
T21783 31394-31396 NN denotes Rb
T21784 31397-31407 NN denotes regulation
T21785 31408-31410 IN denotes of
T21786 31411-31416 NN denotes E2f3a
T21772 31417-31419 VBZ denotes is
T21787 31420-31428 JJ denotes critical
T21788 31429-31431 TO denotes to
T21789 31432-31438 VB denotes ensure
T21790 31439-31445 JJ denotes proper
T21792 31446-31454 JJ denotes neuronal
T21791 31455-31470 NN denotes differentiation
T21793 31470-31471 . denotes .
R10316 T34416 T34417 compound E2f1,Deletion
R10317 T34417 T34418 nsubj Deletion,Rescues
R10318 T34419 T34420 nmod Ganglion,Cells
R10319 T34420 T34418 dobj Cells,Rescues
R10320 T34421 T34419 punct ", ",Ganglion
R10321 T34422 T34419 conj Rod,Ganglion
R10322 T34423 T34422 punct ", ",Rod
R10323 T34424 T34422 cc and,Rod
R10324 T34425 T34422 conj Bipolar,Rod
R10325 T34426 T34418 prep in,Rescues
R10326 T34427 T34428 det the,Retina
R10327 T34428 T34426 pobj Retina,in
R10328 T34429 T34430 compound Rb,KO
R10329 T34430 T34428 compound KO,Retina
R10330 T34432 T34433 punct (,A
R10331 T34433 T34434 meta A,stained
R10332 T34435 T34433 punct ),A
R10333 T34436 T34437 amod Horizontal,sections
R10334 T34437 T34434 nsubjpass sections,stained
R10335 T34438 T34437 amod retinal,sections
R10336 T34439 T34437 prep from,sections
R10337 T34440 T34439 pobj mice,from
R10338 T34441 T34440 prep of,mice
R10339 T34442 T34443 det the,ages
R10340 T34443 T34441 pobj ages,of
R10341 T34444 T34443 amod indicated,ages
R10342 T34445 T34443 cc and,ages
R10343 T34446 T34443 conj genotypes,ages
R10344 T34447 T34434 auxpass were,stained
R10345 T34448 T34434 prep for,stained
R10346 T34449 T34448 pobj nuclei,for
R10347 T34450 T34451 punct (,blue
R10348 T34451 T34449 parataxis blue,nuclei
R10349 T34452 T34451 dep DAPI,blue
R10350 T34453 T34451 punct ", ",blue
R10351 T34454 T34451 punct ),blue
R10352 T34455 T34449 cc and,nuclei
R10353 T34456 T34449 conj markers,nuclei
R10354 T34457 T34458 dep that,detect
R10355 T34458 T34456 relcl detect,markers
R10356 T34459 T34460 compound ganglion,cells
R10357 T34460 T34458 dobj cells,detect
R10358 T34461 T34462 punct (,red
R10359 T34462 T34460 parataxis red,cells
R10360 T34463 T34462 dep Pou4f2,red
R10361 T34464 T34462 punct ", ",red
R10362 T34465 T34462 punct ),red
R10363 T34466 T34460 punct ", ",cells
R10364 T34467 T34460 conj rods,cells
R10365 T34468 T34467 cc and,rods
R10366 T34469 T34467 conj cones,rods
R10367 T34470 T34471 punct (,green
R10368 T34471 T34469 parataxis green,cones
R10369 T34472 T34471 dep Sag,green
R10370 T34473 T34471 punct [,green
R10371 T34474 T34475 compound rod,arrestin
R10372 T34475 T34471 dep arrestin,green
R10373 T34476 T34471 punct ],green
R10374 T34477 T34471 punct ", ",green
R10375 T34478 T34471 punct ),green
R10376 T34479 T34467 punct ", ",rods
R10377 T34480 T34467 cc and,rods
R10378 T34481 T34482 nmod rod,cells
R10379 T34482 T34467 conj cells,rods
R10380 T34483 T34482 amod bipolar,cells
R10381 T34484 T34485 punct (,green
R10382 T34485 T34482 parataxis green,cells
R10383 T34486 T34485 dep Prkca,green
R10384 T34487 T34485 punct ", ",green
R10385 T34488 T34485 punct ", ",green
R10386 T34489 T34485 cc and,green
R10387 T34490 T34491 dep Cabp5,red
R10388 T34491 T34485 conj red,green
R10389 T34492 T34491 punct ", ",red
R10390 T34493 T34491 punct ),red
R10391 T34494 T34434 punct .,stained
R10392 T34496 T34497 compound Scale,bars
R10393 T34497 T34498 nsubj bars,are
R10394 T34499 T34500 nummod 50,μm
R10395 T34500 T34498 attr μm,are
R10396 T34501 T34498 punct .,are
R10397 T34503 T34504 punct (,B
R10398 T34504 T34505 meta B,Quantification
R10399 T34506 T34504 punct ),B
R10400 T34507 T34505 prep of,Quantification
R10401 T34508 T34509 nmod Pou4f2,cells
R10402 T34509 T34507 pobj cells,of
R10403 T34510 T34508 punct +,Pou4f2
R10404 T34511 T34509 compound ganglion,cells
R10405 T34512 T34505 punct .,Quantification
R10406 T34514 T34515 punct (,C
R10407 T34515 T34516 meta C,Quantification
R10408 T34517 T34515 punct ),C
R10409 T34518 T34516 prep of,Quantification
R10410 T34519 T34520 nmod Prkca,cells
R10411 T34520 T34518 pobj cells,of
R10412 T34521 T34519 punct +,Prkca
R10413 T34522 T34519 cc and,Prkca
R10414 T34523 T34519 conj Cabp5,Prkca
R10415 T34524 T34523 punct +,Cabp5
R10416 T34525 T34520 amod bipolar,cells
R10417 T34526 T34516 punct .,Quantification
R10418 T34528 T34529 punct (,D
R10419 T34529 T34530 meta D,Thickness
R10420 T34531 T34529 punct ),D
R10421 T34532 T34530 prep of,Thickness
R10422 T34533 T34534 det the,ONL
R10423 T34534 T34532 pobj ONL,of
R10424 T34535 T34534 punct ", ",ONL
R10425 T34536 T34537 dep which,represents
R10426 T34537 T34534 relcl represents,ONL
R10427 T34538 T34539 det the,number
R10428 T34539 T34537 dobj number,represents
R10429 T34540 T34539 prep of,number
R10430 T34541 T34540 pobj rods,of
R10431 T34542 T34530 punct .,Thickness
R10432 T34544 T34545 compound Error,bars
R10433 T34545 T34546 nsubj bars,represent
R10434 T34547 T34546 dobj SD,represent
R10435 T34548 T34547 prep of,SD
R10436 T34549 T34548 pobj measurements,of
R10437 T34550 T34549 prep from,measurements
R10438 T34551 T34552 nummod three,animals
R10439 T34552 T34550 pobj animals,from
R10440 T34553 T34546 punct ", ",represent
R10441 T34554 T34546 cc and,represent
R10442 T34555 T34556 nsubj asterisks,indicate
R10443 T34556 T34546 conj indicate,represent
R10444 T34557 T34558 det a,difference
R10445 T34558 T34556 dobj difference,indicate
R10446 T34559 T34558 amod significant,difference
R10447 T34560 T34558 prep between,difference
R10448 T34561 T34560 pobj retinas,between
R10449 T34562 T34561 prep of,retinas
R10450 T34563 T34562 pobj WT,of
R10451 T34564 T34563 cc and,WT
R10452 T34565 T34566 det the,indicated
R10453 T34566 T34567 nmod indicated,genotypes
R10454 T34567 T34563 conj genotypes,WT
R10455 T34568 T34556 punct ", ",indicate
R10456 T34569 T34570 mark unless,indicated
R10457 T34570 T34556 advcl indicated,indicate
R10458 T34571 T34570 advmod otherwise,indicated
R10459 T34572 T34570 agent by,indicated
R10460 T34573 T34574 amod connecting,lines
R10461 T34574 T34572 pobj lines,by
R10462 T34575 T34576 punct (,test
R10463 T34576 T34556 parataxis test,indicate
R10464 T34577 T34578 punct *,0.05
R10465 T34578 T34576 ccomp 0.05,test
R10466 T34579 T34578 punct ", ",0.05
R10467 T34580 T34578 nsubj p,0.05
R10468 T34581 T34578 punct <,0.05
R10469 T34582 T34576 punct ;,test
R10470 T34583 T34584 punct **,0.01
R10471 T34584 T34576 ccomp 0.01,test
R10472 T34585 T34584 punct ;,0.01
R10473 T34586 T34584 nsubj p,0.01
R10474 T34587 T34584 punct <,0.01
R10475 T34588 T34576 punct ;,test
R10476 T34589 T34576 nmod ANOVA,test
R10477 T34590 T34589 cc and,ANOVA
R10478 T34591 T34592 compound Tukey,HSD
R10479 T34592 T34589 conj HSD,ANOVA
R10480 T34593 T34576 punct ),test
R10481 T34594 T34556 punct .,indicate
R10482 T34596 T34597 punct (,E
R10483 T34597 T34598 meta E,recorded
R10484 T34599 T34597 cc and,E
R10485 T34600 T34597 conj F,E
R10486 T34601 T34600 punct ),F
R10487 T34602 T34598 nsubjpass ERGs,recorded
R10488 T34603 T34598 auxpass were,recorded
R10489 T34604 T34598 prep from,recorded
R10490 T34605 T34606 det the,genotypes
R10491 T34606 T34604 pobj genotypes,from
R10492 T34607 T34606 amod indicated,genotypes
R10493 T34608 T34598 prep under,recorded
R10494 T34609 T34610 npadvmod dark,adapted
R10495 T34610 T34612 amod adapted,conditions
R10496 T34611 T34610 punct -,adapted
R10497 T34612 T34608 pobj conditions,under
R10498 T34613 T34614 punct (,scotopic
R10499 T34614 T34610 parataxis scotopic,adapted
R10500 T34615 T34614 punct ),scotopic
R10501 T34616 T34598 punct ", ",recorded
R10502 T34617 T34598 cc and,recorded
R10503 T34618 T34619 punct (,E
R10504 T34619 T34620 parataxis E,series
R10505 T34620 T34623 nsubjpass series,determined
R10506 T34621 T34619 punct ),E
R10507 T34622 T34620 compound intensity,series
R10508 T34623 T34598 conj determined,recorded
R10509 T34624 T34620 cc and,series
R10510 T34625 T34626 punct (,F
R10511 T34626 T34627 parataxis F,amplitudes
R10512 T34627 T34620 conj amplitudes,series
R10513 T34628 T34626 punct ),F
R10514 T34629 T34630 compound b,wave
R10515 T34630 T34627 compound wave,amplitudes
R10516 T34631 T34630 punct -,wave
R10517 T34632 T34620 prep as,series
R10518 T34633 T34634 det a,function
R10519 T34634 T34632 pobj function,as
R10520 T34635 T34634 prep of,function
R10521 T34636 T34637 det the,logarithm
R10522 T34637 T34635 pobj logarithm,of
R10523 T34638 T34637 prep of,logarithm
R10524 T34639 T34640 det the,intensity
R10525 T34640 T34638 pobj intensity,of
R10526 T34641 T34640 compound flash,intensity
R10527 T34642 T34623 auxpass were,determined
R10528 T34643 T34623 punct .,determined
R10529 T34645 T34646 punct (,illustrates
R10530 T34647 T34646 nsubj F,illustrates
R10531 T34648 T34646 punct ),illustrates
R10532 T34649 T34646 advmod Further,illustrates
R10533 T34650 T34651 mark that,was
R10534 T34651 T34646 ccomp was,illustrates
R10535 T34652 T34653 det the,influence
R10536 T34653 T34651 nsubj influence,was
R10537 T34654 T34653 amod relative,influence
R10538 T34655 T34653 prep of,influence
R10539 T34656 T34657 det the,mutations
R10540 T34657 T34655 pobj mutations,of
R10541 T34658 T34653 prep on,influence
R10542 T34659 T34660 det the,photoreceptors
R10543 T34660 T34658 pobj photoreceptors,on
R10544 T34661 T34660 punct (,photoreceptors
R10545 T34662 T34660 acl indicated,photoreceptors
R10546 T34663 T34662 agent by,indicated
R10547 T34664 T34665 det the,amplitude
R10548 T34665 T34663 pobj amplitude,by
R10549 T34666 T34665 amod saturated,amplitude
R10550 T34667 T34668 compound a,wave
R10551 T34668 T34665 compound wave,amplitude
R10552 T34669 T34668 punct -,wave
R10553 T34670 T34671 punct ", ",graph
R10554 T34671 T34662 parataxis graph,indicated
R10555 T34672 T34671 amod right,graph
R10556 T34673 T34671 punct ),graph
R10557 T34674 T34651 neg not,was
R10558 T34675 T34676 advmod substantially,different
R10559 T34676 T34651 acomp different,was
R10560 T34677 T34676 prep from,different
R10561 T34678 T34679 poss their,effect
R10562 T34679 T34677 pobj effect,from
R10563 T34680 T34679 prep on,effect
R10564 T34681 T34682 det the,response
R10565 T34682 T34680 pobj response,on
R10566 T34683 T34684 compound b,wave
R10567 T34684 T34682 compound wave,response
R10568 T34685 T34684 punct -,wave
R10569 T34686 T34679 punct (,effect
R10570 T34687 T34679 acl dominated,effect
R10571 T34688 T34687 agent by,dominated
R10572 T34689 T34690 det the,bipolars
R10573 T34690 T34688 pobj bipolars,by
R10574 T34691 T34692 punct ", ",graph
R10575 T34692 T34690 parataxis graph,bipolars
R10576 T34693 T34692 amod left,graph
R10577 T34694 T34692 punct ),graph
R10578 T34695 T34690 prep at,bipolars
R10579 T34696 T34697 det the,intensity
R10580 T34697 T34695 pobj intensity,at
R10581 T34698 T34697 amod same,intensity
R10582 T34699 T34697 prep of,intensity
R10583 T34700 T34701 nummod 10,s
R10584 T34701 T34699 pobj s,of
R10585 T34702 T34701 nmod cd,s
R10586 T34703 T34701 punct ·,s
R10587 T34704 T34705 punct /,m2
R10588 T34705 T34701 prep m2,s
R10589 T34706 T34646 punct .,illustrates
R10590 T35125 T35126 compound Differentiation,Defects
R10591 T35127 T35126 prep in,Defects
R10592 T35128 T35129 compound Rb,KO
R10593 T35129 T35130 compound KO,SACs
R10594 T35130 T35127 pobj SACs,in
R10595 T35132 T35133 punct (,A
R10596 T35133 T35134 meta A,stained
R10597 T35135 T35133 punct ),A
R10598 T35136 T35137 nmod P18,sections
R10599 T35137 T35134 nsubjpass sections,stained
R10600 T35138 T35137 amod horizontal,sections
R10601 T35139 T35137 prep of,sections
R10602 T35140 T35141 nmod WT,retina
R10603 T35141 T35139 pobj retina,of
R10604 T35142 T35140 punct ", ",WT
R10605 T35143 T35144 compound Rb,KO
R10606 T35144 T35140 conj KO,WT
R10607 T35145 T35144 punct ", ",KO
R10608 T35146 T35144 cc and,KO
R10609 T35147 T35148 compound Rb,E2f1
R10610 T35148 T35150 compound E2f1,DKO
R10611 T35149 T35148 punct /,E2f1
R10612 T35150 T35144 conj DKO,KO
R10613 T35151 T35134 auxpass were,stained
R10614 T35152 T35134 prep for,stained
R10615 T35153 T35152 pobj nuclei,for
R10616 T35154 T35155 punct (,blue
R10617 T35155 T35153 parataxis blue,nuclei
R10618 T35156 T35155 dep DAPI,blue
R10619 T35157 T35155 punct ", ",blue
R10620 T35158 T35155 punct ),blue
R10621 T35159 T35153 punct ", ",nuclei
R10622 T35160 T35153 conj Calb2,nuclei
R10623 T35161 T35162 punct (,green
R10624 T35162 T35160 parataxis green,Calb2
R10625 T35163 T35162 punct ),green
R10626 T35164 T35160 punct ", ",Calb2
R10627 T35165 T35160 cc and,Calb2
R10628 T35166 T35160 conj Slc18a3,Calb2
R10629 T35167 T35168 punct (,red
R10630 T35168 T35166 parataxis red,Slc18a3
R10631 T35169 T35168 punct ),red
R10632 T35170 T35134 punct .,stained
R10633 T35172 T35173 punct (,B
R10634 T35173 T35174 meta B,stained
R10635 T35175 T35173 punct ),B
R10636 T35176 T35177 amod Confocal,images
R10637 T35177 T35174 nsubjpass images,stained
R10638 T35178 T35177 prep of,images
R10639 T35179 T35180 nmod P30,sections
R10640 T35180 T35178 pobj sections,of
R10641 T35181 T35180 amod horizontal,sections
R10642 T35182 T35180 prep of,sections
R10643 T35183 T35184 nmod WT,retina
R10644 T35184 T35182 pobj retina,of
R10645 T35185 T35183 cc and,WT
R10646 T35186 T35187 compound Rb,KO
R10647 T35187 T35183 conj KO,WT
R10648 T35188 T35174 auxpass were,stained
R10649 T35189 T35174 prep for,stained
R10650 T35190 T35189 pobj nuclei,for
R10651 T35191 T35192 punct (,DAPI
R10652 T35192 T35190 parataxis DAPI,nuclei
R10653 T35193 T35192 punct ", ",DAPI
R10654 T35194 T35192 amod blue,DAPI
R10655 T35195 T35192 punct ),DAPI
R10656 T35196 T35190 punct ", ",nuclei
R10657 T35197 T35190 conj Chat,nuclei
R10658 T35198 T35197 cc and,Chat
R10659 T35199 T35197 conj Slc18a3,Chat
R10660 T35200 T35201 punct (,red
R10661 T35201 T35199 parataxis red,Slc18a3
R10662 T35202 T35201 advmod both,red
R10663 T35203 T35201 punct ),red
R10664 T35204 T35197 punct ", ",Chat
R10665 T35205 T35197 cc and,Chat
R10666 T35206 T35197 conj Camk2a,Chat
R10667 T35207 T35208 punct (,green
R10668 T35208 T35206 parataxis green,Camk2a
R10669 T35209 T35208 punct ),green
R10670 T35210 T35174 punct .,stained
R10671 T35212 T35213 prep In,is
R10672 T35214 T35215 det the,section
R10673 T35215 T35212 pobj section,In
R10674 T35216 T35217 compound Rb,KO
R10675 T35217 T35215 compound KO,section
R10676 T35218 T35213 punct ", ",is
R10677 T35219 T35220 det the,stain
R10678 T35220 T35213 nsubj stain,is
R10679 T35221 T35220 amod red,stain
R10680 T35222 T35213 attr Chat,is
R10681 T35223 T35222 advmod only,Chat
R10682 T35224 T35213 punct ", ",is
R10683 T35225 T35226 mark as,missing
R10684 T35226 T35213 advcl missing,is
R10685 T35227 T35226 nsubj Slc18a3,missing
R10686 T35228 T35226 aux is,missing
R10687 T35229 T35230 punct (,see
R10688 T35230 T35213 parataxis see,is
R10689 T35231 T35230 punct [,see
R10690 T35232 T35230 dobj A,see
R10691 T35233 T35230 punct ],see
R10692 T35234 T35230 punct ),see
R10693 T35235 T35213 punct .,is
R10694 T35237 T35238 punct (,C
R10695 T35238 T35239 meta C,Quantification
R10696 T35240 T35238 punct ),C
R10697 T35241 T35239 prep of,Quantification
R10698 T35242 T35243 amod dense,bodies
R10699 T35243 T35241 pobj bodies,of
R10700 T35244 T35243 nmod Calb2,bodies
R10701 T35245 T35244 punct +,Calb2
R10702 T35246 T35243 compound cell,bodies
R10703 T35247 T35243 prep in,bodies
R10704 T35248 T35249 det the,INL
R10705 T35249 T35247 pobj INL,in
R10706 T35250 T35243 punct ", ",bodies
R10707 T35251 T35252 amod total,bodies
R10708 T35252 T35243 conj bodies,bodies
R10709 T35253 T35252 nmod Slc18a3,bodies
R10710 T35254 T35253 punct +,Slc18a3
R10711 T35255 T35252 compound cell,bodies
R10712 T35256 T35252 punct ", ",bodies
R10713 T35257 T35252 cc and,bodies
R10714 T35258 T35259 nmod Camk2a,bodies
R10715 T35259 T35252 conj bodies,bodies
R10716 T35260 T35258 punct +,Camk2a
R10717 T35261 T35259 compound cell,bodies
R10718 T35262 T35259 prep in,bodies
R10719 T35263 T35264 det the,INL
R10720 T35264 T35262 pobj INL,in
R10721 T35265 T35239 punct .,Quantification
R10722 T35267 T35268 compound Error,bars
R10723 T35268 T35269 nsubj bars,represent
R10724 T35270 T35269 dobj SD,represent
R10725 T35271 T35270 prep of,SD
R10726 T35272 T35271 pobj measurements,of
R10727 T35273 T35272 prep from,measurements
R10728 T35274 T35275 nummod three,animals
R10729 T35275 T35273 pobj animals,from
R10730 T35276 T35269 punct ", ",represent
R10731 T35277 T35269 cc and,represent
R10732 T35278 T35279 nsubj asterisks,indicate
R10733 T35279 T35269 conj indicate,represent
R10734 T35280 T35281 amod significant,differences
R10735 T35281 T35279 dobj differences,indicate
R10736 T35282 T35281 prep between,differences
R10737 T35283 T35282 pobj retinas,between
R10738 T35284 T35283 prep of,retinas
R10739 T35285 T35284 pobj WT,of
R10740 T35286 T35285 cc and,WT
R10741 T35287 T35288 det the,genotypes
R10742 T35288 T35285 conj genotypes,WT
R10743 T35289 T35288 amod indicated,genotypes
R10744 T35290 T35291 punct (,test
R10745 T35291 T35279 parataxis test,indicate
R10746 T35292 T35293 punct *,0.05
R10747 T35293 T35291 ccomp 0.05,test
R10748 T35294 T35293 punct ", ",0.05
R10749 T35295 T35293 nsubj p,0.05
R10750 T35296 T35293 punct <,0.05
R10751 T35297 T35291 punct ;,test
R10752 T35298 T35299 punct **,0.01
R10753 T35299 T35291 ccomp 0.01,test
R10754 T35300 T35299 punct ;,0.01
R10755 T35301 T35299 nsubj p,0.01
R10756 T35302 T35299 punct <,0.01
R10757 T35303 T35291 punct ;,test
R10758 T35304 T35291 nmod ANOVA,test
R10759 T35305 T35304 cc and,ANOVA
R10760 T35306 T35307 compound Tukey,HSD
R10761 T35307 T35304 conj HSD,ANOVA
R10762 T35308 T35291 punct ),test
R10763 T35309 T35279 punct .,indicate
R10764 T35311 T35312 compound Scale,bars
R10765 T35312 T35313 nsubj bars,are
R10766 T35314 T35315 nummod 50,μm
R10767 T35315 T35313 attr μm,are
R10768 T35316 T35315 prep in,μm
R10769 T35317 T35316 punct (,in
R10770 T35318 T35316 pobj A,in
R10771 T35319 T35315 punct ),μm
R10772 T35320 T35315 cc and,μm
R10773 T35321 T35322 nummod 20,μm
R10774 T35322 T35315 conj μm,μm
R10775 T35323 T35322 prep in,μm
R10776 T35324 T35323 punct (,in
R10777 T35325 T35323 pobj B,in
R10778 T35326 T35313 punct ),are
R10779 T35327 T35313 punct .,are
R10780 T36048 T36049 compound E2f3,Loss
R10781 T36049 T36050 nsubj Loss,Rescues
R10782 T36051 T36050 dobj Differentiation,Rescues
R10783 T36052 T36051 prep of,Differentiation
R10784 T36053 T36054 compound Rb,KO
R10785 T36054 T36055 compound KO,SACs
R10786 T36055 T36052 pobj SACs,of
R10787 T36057 T36058 punct (,A
R10788 T36058 T36059 meta A,stained
R10789 T36060 T36058 punct ),A
R10790 T36061 T36062 amod Horizontal,sections
R10791 T36062 T36059 nsubjpass sections,stained
R10792 T36063 T36062 amod retinal,sections
R10793 T36064 T36062 prep of,sections
R10794 T36065 T36066 det the,ages
R10795 T36066 T36064 pobj ages,of
R10796 T36067 T36066 amod indicated,ages
R10797 T36068 T36066 cc and,ages
R10798 T36069 T36066 conj genotypes,ages
R10799 T36070 T36059 auxpass were,stained
R10800 T36071 T36059 prep for,stained
R10801 T36072 T36071 pobj nuclei,for
R10802 T36073 T36074 punct (,blue
R10803 T36074 T36072 parataxis blue,nuclei
R10804 T36075 T36074 dep DAPI,blue
R10805 T36076 T36074 punct ", ",blue
R10806 T36077 T36074 punct ),blue
R10807 T36078 T36072 punct ", ",nuclei
R10808 T36079 T36080 compound mitosis,marker
R10809 T36080 T36081 compound marker,PH3
R10810 T36081 T36072 conj PH3,nuclei
R10811 T36082 T36083 punct (,green
R10812 T36083 T36081 parataxis green,PH3
R10813 T36084 T36083 punct ),green
R10814 T36085 T36081 punct ", ",PH3
R10815 T36086 T36081 cc and,PH3
R10816 T36087 T36081 conj Slc18a3,PH3
R10817 T36088 T36089 punct (,red
R10818 T36089 T36087 parataxis red,Slc18a3
R10819 T36090 T36089 punct ),red
R10820 T36091 T36087 punct ", ",Slc18a3
R10821 T36092 T36093 dep which,marks
R10822 T36093 T36087 relcl marks,Slc18a3
R10823 T36094 T36095 compound SAC,soma
R10824 T36095 T36093 dobj soma,marks
R10825 T36096 T36093 prep at,marks
R10826 T36097 T36098 amod early,stages
R10827 T36098 T36096 pobj stages,at
R10828 T36099 T36093 cc and,marks
R10829 T36100 T36093 conj processes,marks
R10830 T36101 T36100 prep from,processes
R10831 T36102 T36103 punct ~,P5
R10832 T36103 T36101 pobj P5,from
R10833 T36104 T36103 advmod onwards,P5
R10834 T36105 T36059 punct .,stained
R10835 T36107 T36108 nsubj Arrows,show
R10836 T36109 T36110 amod mitotic,nuclei
R10837 T36110 T36108 dobj nuclei,show
R10838 T36111 T36110 nmod PH3,nuclei
R10839 T36112 T36111 punct +,PH3
R10840 T36113 T36108 prep in,show
R10841 T36114 T36115 nmod Rb,KO
R10842 T36115 T36116 nmod KO,retinas
R10843 T36116 T36113 pobj retinas,in
R10844 T36117 T36115 punct ", ",KO
R10845 T36118 T36119 compound Rb,E2f2
R10846 T36119 T36121 compound E2f2,DKO
R10847 T36120 T36119 punct /,E2f2
R10848 T36121 T36115 conj DKO,KO
R10849 T36122 T36121 punct ", ",DKO
R10850 T36123 T36121 cc and,DKO
R10851 T36124 T36125 compound Rb,E2f3
R10852 T36125 T36127 compound E2f3,DKO
R10853 T36126 T36125 punct /,E2f3
R10854 T36127 T36121 conj DKO,DKO
R10855 T36128 T36108 punct .,show
R10856 T36130 T36131 compound E2f1,loss
R10857 T36131 T36132 nsubj loss,rescues
R10858 T36133 T36134 det the,defects
R10859 T36134 T36132 dobj defects,rescues
R10860 T36135 T36136 amod ectopic,mitosis
R10861 T36136 T36134 nmod mitosis,defects
R10862 T36137 T36136 cc and,mitosis
R10863 T36138 T36139 compound cell,death
R10864 T36139 T36136 conj death,mitosis
R10865 T36140 T36134 punct ", ",defects
R10866 T36141 T36134 cc but,defects
R10867 T36142 T36141 neg not,but
R10868 T36143 T36144 det the,defect
R10869 T36144 T36134 conj defect,defects
R10870 T36145 T36144 compound SAC,defect
R10871 T36146 T36132 punct .,rescues
R10872 T36148 T36149 compound E2f2,loss
R10873 T36149 T36150 nsubj loss,has
R10874 T36151 T36152 det no,effect
R10875 T36152 T36150 dobj effect,has
R10876 T36153 T36150 punct .,has
R10877 T36155 T36156 compound E2f3,loss
R10878 T36156 T36157 nsubj loss,rescue
R10879 T36158 T36157 aux does,rescue
R10880 T36159 T36157 neg not,rescue
R10881 T36160 T36161 det the,defects
R10882 T36161 T36157 dobj defects,rescue
R10883 T36162 T36163 amod ectopic,mitosis
R10884 T36163 T36161 nmod mitosis,defects
R10885 T36164 T36163 cc and,mitosis
R10886 T36165 T36166 compound cell,loss
R10887 T36166 T36163 conj loss,mitosis
R10888 T36167 T36157 punct ", ",rescue
R10889 T36168 T36157 cc but,rescue
R10890 T36169 T36157 conj rescues,rescue
R10891 T36170 T36171 det the,defect
R10892 T36171 T36169 dobj defect,rescues
R10893 T36172 T36171 compound SAC,defect
R10894 T36173 T36157 punct .,rescue
R10895 T36175 T36176 csubj Inactivating,rescues
R10896 T36177 T36175 dobj E2f1,Inactivating
R10897 T36178 T36177 cc and,E2f1
R10898 T36179 T36177 conj E2f3,E2f1
R10899 T36180 T36175 advmod together,Inactivating
R10900 T36181 T36182 det the,defects
R10901 T36182 T36176 dobj defects,rescues
R10902 T36183 T36184 amod ectopic,mitosis
R10903 T36184 T36182 nmod mitosis,defects
R10904 T36185 T36184 punct ", ",mitosis
R10905 T36186 T36187 compound cell,death
R10906 T36187 T36184 conj death,mitosis
R10907 T36188 T36187 punct ", ",death
R10908 T36189 T36187 cc and,death
R10909 T36190 T36187 conj SAC,death
R10910 T36191 T36176 punct .,rescues
R10911 T36193 T36194 punct (,B
R10912 T36194 T36195 meta B,fraction
R10913 T36196 T36194 punct ),B
R10914 T36197 T36195 det The,fraction
R10915 T36198 T36195 prep of,fraction
R10916 T36199 T36200 nmod Camk2a,cells
R10917 T36200 T36198 pobj cells,of
R10918 T36201 T36199 punct +,Camk2a
R10919 T36202 T36203 dep that,are
R10920 T36203 T36200 relcl are,cells
R10921 T36204 T36203 attr Chat,are
R10922 T36205 T36204 punct +,Chat
R10923 T36206 T36204 cc and,Chat
R10924 T36207 T36204 conj Slc18a3,Chat
R10925 T36208 T36207 punct +,Slc18a3
R10926 T36209 T36195 prep in,fraction
R10927 T36210 T36211 det the,retina
R10928 T36211 T36209 pobj retina,in
R10929 T36212 T36211 compound P30,retina
R10930 T36213 T36195 punct .,fraction
R10931 T36215 T36216 punct (,C
R10932 T36216 T36217 meta C,stained
R10933 T36218 T36216 punct ),C
R10934 T36219 T36220 amod Horizontal,sections
R10935 T36220 T36217 nsubjpass sections,stained
R10936 T36221 T36220 compound P5,sections
R10937 T36222 T36220 compound retinal,sections
R10938 T36223 T36217 auxpass were,stained
R10939 T36224 T36217 prep for,stained
R10940 T36225 T36224 pobj nuclei,for
R10941 T36226 T36227 punct (,blue
R10942 T36227 T36225 parataxis blue,nuclei
R10943 T36228 T36227 dep DAPI,blue
R10944 T36229 T36227 punct ", ",blue
R10945 T36230 T36227 punct ),blue
R10946 T36231 T36225 punct ", ",nuclei
R10947 T36232 T36225 conj Slc18a3,nuclei
R10948 T36233 T36234 punct (,green
R10949 T36234 T36232 parataxis green,Slc18a3
R10950 T36235 T36234 punct ),green
R10951 T36236 T36232 punct ", ",Slc18a3
R10952 T36237 T36232 cc and,Slc18a3
R10953 T36238 T36232 conj Isl1,Slc18a3
R10954 T36239 T36240 punct (,red
R10955 T36240 T36238 parataxis red,Isl1
R10956 T36241 T36240 punct ),red
R10957 T36242 T36217 punct .,stained
R10958 T36244 T36245 nsubj Arrows,show
R10959 T36246 T36247 amod double,labelled
R10960 T36247 T36249 amod labelled,cells
R10961 T36248 T36247 punct -,labelled
R10962 T36249 T36245 dobj cells,show
R10963 T36250 T36249 nmod Isl1,cells
R10964 T36251 T36250 punct +,Isl1
R10965 T36252 T36250 punct /,Isl1
R10966 T36253 T36250 appos Slc18a3,Isl1
R10967 T36254 T36253 punct +,Slc18a3
R10968 T36255 T36245 prep in,show
R10969 T36256 T36257 det the,INL
R10970 T36257 T36255 pobj INL,in
R10971 T36258 T36257 amod inner,INL
R10972 T36259 T36245 punct .,show
R10973 T36261 T36262 punct (,D
R10974 T36262 T36263 meta D,stained
R10975 T36264 T36262 punct ),D
R10976 T36265 T36266 amod Horizontal,sections
R10977 T36266 T36263 nsubjpass sections,stained
R10978 T36267 T36266 nmod P0,sections
R10979 T36268 T36266 amod retinal,sections
R10980 T36269 T36266 prep of,sections
R10981 T36270 T36271 det the,genotypes
R10982 T36271 T36269 pobj genotypes,of
R10983 T36272 T36271 amod indicated,genotypes
R10984 T36273 T36263 auxpass were,stained
R10985 T36274 T36263 prep for,stained
R10986 T36275 T36274 pobj nuclei,for
R10987 T36276 T36277 punct (,blue
R10988 T36277 T36275 parataxis blue,nuclei
R10989 T36278 T36277 dep DAPI,blue
R10990 T36279 T36277 punct ", ",blue
R10991 T36280 T36277 punct ),blue
R10992 T36281 T36275 punct ", ",nuclei
R10993 T36282 T36283 compound cell,division
R10994 T36283 T36284 compound division,marker
R10995 T36284 T36285 compound marker,Mki67
R10996 T36285 T36275 conj Mki67,nuclei
R10997 T36286 T36287 punct (,green
R10998 T36287 T36285 parataxis green,Mki67
R10999 T36288 T36287 punct ),green
R11000 T36289 T36285 punct ", ",Mki67
R11001 T36290 T36285 cc and,Mki67
R11002 T36291 T36285 conj Isl1,Mki67
R11003 T36292 T36293 punct (,red
R11004 T36293 T36291 parataxis red,Isl1
R11005 T36294 T36293 punct ),red
R11006 T36295 T36263 punct .,stained
R11007 T36297 T36298 nsubj Arrows,show
R11008 T36299 T36300 amod double,labelled
R11009 T36300 T36302 amod labelled,SACs
R11010 T36301 T36300 punct -,labelled
R11011 T36302 T36298 dobj SACs,show
R11012 T36303 T36302 amod dividing,SACs
R11013 T36304 T36298 punct .,show
R11014 T36306 T36307 punct (,E
R11015 T36307 T36308 meta E,fraction
R11016 T36309 T36307 punct ),E
R11017 T36310 T36308 det The,fraction
R11018 T36311 T36308 prep of,fraction
R11019 T36312 T36313 nmod Isl1,cells
R11020 T36313 T36311 pobj cells,of
R11021 T36314 T36312 punct +,Isl1
R11022 T36315 T36313 prep in,cells
R11023 T36316 T36317 det the,NBL
R11024 T36317 T36315 pobj NBL,in
R11025 T36318 T36317 amod inner,NBL
R11026 T36319 T36317 punct (,NBL
R11027 T36320 T36317 appos INBL,NBL
R11028 T36321 T36317 punct ),NBL
R11029 T36322 T36317 prep of,NBL
R11030 T36323 T36324 compound P0,retinas
R11031 T36324 T36322 pobj retinas,of
R11032 T36325 T36326 dep that,dividing
R11033 T36326 T36313 relcl dividing,cells
R11034 T36327 T36326 aux are,dividing
R11035 T36328 T36329 punct (,Mki67
R11036 T36329 T36326 parataxis Mki67,dividing
R11037 T36330 T36329 punct +,Mki67
R11038 T36331 T36329 punct ),Mki67
R11039 T36332 T36308 punct .,fraction
R11040 T36334 T36335 compound Error,bars
R11041 T36335 T36336 nsubj bars,represent
R11042 T36337 T36336 dobj SD,represent
R11043 T36338 T36337 prep of,SD
R11044 T36339 T36338 pobj measurements,of
R11045 T36340 T36339 prep from,measurements
R11046 T36341 T36342 nummod three,animals
R11047 T36342 T36340 pobj animals,from
R11048 T36343 T36336 punct ", ",represent
R11049 T36344 T36336 cc and,represent
R11050 T36345 T36346 nsubj asterisks,indicate
R11051 T36346 T36336 conj indicate,represent
R11052 T36347 T36348 amod significant,differences
R11053 T36348 T36346 dobj differences,indicate
R11054 T36349 T36348 prep between,differences
R11055 T36350 T36349 pobj retinas,between
R11056 T36351 T36350 prep of,retinas
R11057 T36352 T36351 pobj WT,of
R11058 T36353 T36352 cc and,WT
R11059 T36354 T36355 det the,indicated
R11060 T36355 T36356 nmod indicated,genotypes
R11061 T36356 T36352 conj genotypes,WT
R11062 T36357 T36358 punct (,test
R11063 T36358 T36346 parataxis test,indicate
R11064 T36359 T36360 punct *,0.05
R11065 T36360 T36358 ccomp 0.05,test
R11066 T36361 T36360 punct ", ",0.05
R11067 T36362 T36360 nsubj p,0.05
R11068 T36363 T36360 punct <,0.05
R11069 T36364 T36358 punct ;,test
R11070 T36365 T36366 punct **,0.01
R11071 T36366 T36358 ccomp 0.01,test
R11072 T36367 T36366 punct ;,0.01
R11073 T36368 T36366 nsubj p,0.01
R11074 T36369 T36366 punct <,0.01
R11075 T36370 T36358 punct ;,test
R11076 T36371 T36358 nmod ANOVA,test
R11077 T36372 T36371 cc and,ANOVA
R11078 T36373 T36374 compound Tukey,HSD
R11079 T36374 T36371 conj HSD,ANOVA
R11080 T36375 T36358 punct ),test
R11081 T36376 T36346 punct .,indicate
R11082 T36378 T36379 compound Scale,bars
R11083 T36379 T36380 nsubj bars,are
R11084 T36381 T36379 prep in,bars
R11085 T36382 T36381 punct (,in
R11086 T36383 T36381 pobj A,in
R11087 T36384 T36383 punct ),A
R11088 T36385 T36383 punct ", ",A
R11089 T36386 T36387 punct (,C
R11090 T36387 T36383 conj C,A
R11091 T36388 T36387 punct ),C
R11092 T36389 T36387 punct ", ",C
R11093 T36390 T36387 cc and,C
R11094 T36391 T36392 punct (,D
R11095 T36392 T36387 conj D,C
R11096 T36393 T36380 punct ),are
R11097 T36394 T36395 nummod 50,μm
R11098 T36395 T36380 attr μm,are
R11099 T36396 T36380 punct .,are
R11106 T36780 T36781 nmod E2f3,Expression
R11107 T36782 T36780 cc and,E2f3
R11108 T36783 T36780 conj Rb,E2f3
R11109 T36784 T36781 prep in,Expression
R11110 T36785 T36784 pobj SACs,in
R11111 T36787 T36788 punct (,A
R11112 T36788 T36789 meta A,stained
R11113 T36790 T36788 punct ),A
R11114 T36791 T36792 amod Left,panels
R11115 T36792 T36789 dep panels,stained
R11116 T36793 T36789 punct : ,stained
R11117 T36794 T36795 amod horizontal,sections
R11118 T36795 T36789 nsubjpass sections,stained
R11119 T36796 T36795 nmod P0,sections
R11120 T36797 T36796 punct ", ",P0
R11121 T36798 T36796 conj P8,P0
R11122 T36799 T36798 punct ", ",P8
R11123 T36800 T36798 cc and,P8
R11124 T36801 T36798 conj P18,P8
R11125 T36802 T36795 amod retinal,sections
R11126 T36803 T36795 prep of,sections
R11127 T36804 T36805 det the,genotypes
R11128 T36805 T36803 pobj genotypes,of
R11129 T36806 T36805 amod indicated,genotypes
R11130 T36807 T36789 auxpass were,stained
R11131 T36808 T36789 prep for,stained
R11132 T36809 T36808 pobj E2f3,for
R11133 T36810 T36811 punct (,red
R11134 T36811 T36809 parataxis red,E2f3
R11135 T36812 T36811 punct ),red
R11136 T36813 T36809 cc and,E2f3
R11137 T36814 T36809 conj DAPI,E2f3
R11138 T36815 T36816 punct (,blue
R11139 T36816 T36814 parataxis blue,DAPI
R11140 T36817 T36816 punct ),blue
R11141 T36818 T36789 punct .,stained
R11142 T36820 T36821 det The,arrow
R11143 T36821 T36822 nsubj arrow,indicates
R11144 T36823 T36824 det the,junction
R11145 T36824 T36822 dobj junction,indicates
R11146 T36825 T36824 prep between,junction
R11147 T36826 T36827 det the,peripheral
R11148 T36827 T36825 pobj peripheral,between
R11149 T36828 T36829 npadvmod E2f3,null
R11150 T36829 T36827 amod null,peripheral
R11151 T36830 T36827 cc and,peripheral
R11152 T36831 T36832 nmod WT,retina
R11153 T36832 T36827 conj retina,peripheral
R11154 T36833 T36831 amod central,WT
R11155 T36834 T36832 compound P0,retina
R11156 T36835 T36822 punct .,indicates
R11157 T36838 T36839 det the,absence
R11158 T36839 T36837 dobj absence,Note
R11159 T36840 T36839 prep of,absence
R11160 T36841 T36842 compound E2f3,protein
R11161 T36842 T36840 pobj protein,of
R11162 T36843 T36839 prep in,absence
R11163 T36844 T36845 det the,RPCs
R11164 T36845 T36843 pobj RPCs,in
R11165 T36846 T36845 amod peripheral,RPCs
R11166 T36847 T36848 compound E2f3,KO
R11167 T36848 T36845 compound KO,RPCs
R11168 T36849 T36839 prep at,absence
R11169 T36850 T36849 pobj P0,at
R11170 T36851 T36839 cc and,absence
R11171 T36852 T36853 prep in,at
R11172 T36853 T36839 conj at,absence
R11173 T36854 T36855 amod peripheral,neurons
R11174 T36855 T36852 pobj neurons,in
R11175 T36856 T36855 amod inner,neurons
R11176 T36857 T36855 amod retinal,neurons
R11177 T36858 T36853 pobj P18,at
R11178 T36859 T36837 punct .,Note
R11179 T36861 T36862 advmod Far,right
R11180 T36862 T36863 amod right,panel
R11181 T36863 T36864 dep panel,stained
R11182 T36865 T36864 punct : ,stained
R11183 T36866 T36867 nmod P18,sections
R11184 T36867 T36864 nsubjpass sections,stained
R11185 T36868 T36867 amod retinal,sections
R11186 T36869 T36867 prep of,sections
R11187 T36870 T36871 det the,genotypes
R11188 T36871 T36869 pobj genotypes,of
R11189 T36872 T36871 amod indicated,genotypes
R11190 T36873 T36864 auxpass were,stained
R11191 T36874 T36864 prep for,stained
R11192 T36875 T36874 pobj Rb,for
R11193 T36876 T36877 punct (,red
R11194 T36877 T36875 parataxis red,Rb
R11195 T36878 T36877 punct ),red
R11196 T36879 T36875 cc and,Rb
R11197 T36880 T36875 conj DAPI,Rb
R11198 T36881 T36882 punct (,blue
R11199 T36882 T36880 parataxis blue,DAPI
R11200 T36883 T36882 punct ),blue
R11201 T36884 T36864 punct .,stained
R11202 T36887 T36888 det the,absence
R11203 T36888 T36886 dobj absence,Note
R11204 T36889 T36888 prep of,absence
R11205 T36890 T36891 compound Rb,protein
R11206 T36891 T36889 pobj protein,of
R11207 T36892 T36888 prep in,absence
R11208 T36893 T36894 det the,neurons
R11209 T36894 T36892 pobj neurons,in
R11210 T36895 T36894 amod peripheral,neurons
R11211 T36896 T36897 nmod Rb,KO
R11212 T36897 T36894 nmod KO,neurons
R11213 T36898 T36894 amod inner,neurons
R11214 T36899 T36894 amod retinal,neurons
R11215 T36900 T36886 punct .,Note
R11216 T36902 T36903 punct (,B
R11217 T36903 T36904 meta B,stained
R11218 T36905 T36903 punct ),B
R11219 T36906 T36907 nmod WT,sections
R11220 T36907 T36904 nsubjpass sections,stained
R11221 T36908 T36907 nmod P18,sections
R11222 T36909 T36907 amod retinal,sections
R11223 T36910 T36904 auxpass were,stained
R11224 T36911 T36904 prep for,stained
R11225 T36912 T36911 pobj nuclei,for
R11226 T36913 T36914 punct (,blue
R11227 T36914 T36912 parataxis blue,nuclei
R11228 T36915 T36914 dep DAPI,blue
R11229 T36916 T36914 punct ", ",blue
R11230 T36917 T36914 punct ),blue
R11231 T36918 T36912 punct ", ",nuclei
R11232 T36919 T36912 conj E2f3,nuclei
R11233 T36920 T36921 punct (,red
R11234 T36921 T36919 parataxis red,E2f3
R11235 T36922 T36921 punct ),red
R11236 T36923 T36919 cc or,E2f3
R11237 T36924 T36919 conj Rb,E2f3
R11238 T36925 T36926 punct (,red
R11239 T36926 T36924 parataxis red,Rb
R11240 T36927 T36926 punct ),red
R11241 T36928 T36924 punct ", ",Rb
R11242 T36929 T36924 cc and,Rb
R11243 T36930 T36924 conj Chat,Rb
R11244 T36931 T36930 cc plus,Chat
R11245 T36932 T36930 conj Slc18a3,Chat
R11246 T36933 T36934 punct (,green
R11247 T36934 T36932 parataxis green,Slc18a3
R11248 T36935 T36934 punct ),green
R11249 T36936 T36904 punct .,stained
R11250 T36938 T36939 nsubj Arrows,indicate
R11251 T36940 T36941 amod double,labelled
R11252 T36941 T36943 amod labelled,soma
R11253 T36942 T36941 punct -,labelled
R11254 T36943 T36939 dobj soma,indicate
R11255 T36944 T36939 punct .,indicate
R11256 T36947 T36948 mark that,labelled
R11257 T36948 T36946 ccomp labelled,Note
R11258 T36949 T36950 det the,processes
R11259 T36950 T36948 nsubjpass processes,labelled
R11260 T36951 T36950 compound IPL,processes
R11261 T36952 T36948 auxpass are,labelled
R11262 T36953 T36948 advmod also,labelled
R11263 T36954 T36948 amod double,labelled
R11264 T36955 T36948 punct -,labelled
R11265 T36956 T36946 punct .,Note
R11266 T36958 T36959 compound Scale,bars
R11267 T36959 T36960 nsubj bars,are
R11268 T36961 T36962 nummod 50,μm
R11269 T36962 T36960 attr μm,are
R11270 T36963 T36960 punct .,are
R11271 T37145 T37146 amod Subcellular,Distribution
R11272 T37147 T37146 prep of,Distribution
R11273 T37148 T37149 compound E2f3,Isoforms
R11274 T37149 T37147 pobj Isoforms,of
R11275 T37150 T37149 cc and,Isoforms
R11276 T37151 T37152 amod Other,Proteins
R11277 T37152 T37149 conj Proteins,Isoforms
R11278 T37153 T37154 compound Cell,Cycle
R11279 T37154 T37152 compound Cycle,Proteins
R11280 T37155 T37146 prep in,Distribution
R11281 T37156 T37157 det the,Retina
R11282 T37157 T37155 pobj Retina,in
R11283 T37158 T37157 amod Developing,Retina
R11284 T37160 T37161 amod Nuclear,extracts
R11285 T37161 T37164 nsubjpass extracts,analyzed
R11286 T37162 T37160 cc and,Nuclear
R11287 T37163 T37160 conj cytoplasmic,Nuclear
R11288 T37165 T37161 prep from,extracts
R11289 T37166 T37167 det an,number
R11290 T37167 T37165 pobj number,from
R11291 T37168 T37167 amod equivalent,number
R11292 T37169 T37167 prep of,number
R11293 T37170 T37171 amod retinal,cells
R11294 T37171 T37169 pobj cells,of
R11295 T37172 T37171 prep from,cells
R11296 T37173 T37172 pobj mice,from
R11297 T37174 T37173 prep of,mice
R11298 T37175 T37176 det the,genotypes
R11299 T37176 T37174 pobj genotypes,of
R11300 T37177 T37176 amod indicated,genotypes
R11301 T37178 T37176 cc and,genotypes
R11302 T37179 T37176 conj ages,genotypes
R11303 T37180 T37164 auxpass were,analyzed
R11304 T37181 T37164 prep by,analyzed
R11305 T37182 T37183 compound Western,blotting
R11306 T37183 T37181 pobj blotting,by
R11307 T37184 T37185 aux to,detect
R11308 T37185 T37164 advcl detect,analyzed
R11309 T37186 T37187 det the,proteins
R11310 T37187 T37185 dobj proteins,detect
R11311 T37188 T37187 amod indicated,proteins
R11312 T37189 T37164 punct .,analyzed
R11313 T37191 T37192 nsubjpass Lysate,used
R11314 T37193 T37191 prep from,Lysate
R11315 T37194 T37195 nmod E2f3a,mice
R11316 T37195 T37193 pobj mice,from
R11317 T37196 T37194 punct −,E2f3a
R11318 T37197 T37194 punct /,E2f3a
R11319 T37198 T37194 punct −,E2f3a
R11320 T37199 T37192 auxpass was,used
R11321 T37200 T37192 prep as,used
R11322 T37201 T37202 det a,control
R11323 T37202 T37200 pobj control,as
R11324 T37203 T37204 aux to,confirm
R11325 T37204 T37192 advcl confirm,used
R11326 T37205 T37206 det the,location
R11327 T37206 T37204 dobj location,confirm
R11328 T37207 T37206 prep of,location
R11329 T37208 T37209 compound E2f3a,protein
R11330 T37209 T37207 pobj protein,of
R11331 T37210 T37192 punct .,used
R11332 T37213 T37212 punct ", ",C
R11333 T37214 T37215 amod cytoplasmic,extracts
R11334 T37215 T37212 appos extracts,C
R11335 T37216 T37212 punct ;,C
R11336 T37217 T37212 appos N,C
R11337 T37218 T37217 punct ", ",N
R11338 T37219 T37220 amod nuclear,extracts
R11339 T37220 T37217 appos extracts,N
R11340 T37221 T37212 punct .,C
R11341 T37915 T37916 det The,Isoform
R11342 T37916 T37918 nsubj Isoform,Drives
R11343 T37917 T37916 compound E2f3a,Isoform
R11344 T37919 T37920 det the,Defect
R11345 T37920 T37918 dobj Defect,Drives
R11346 T37921 T37920 compound Differentiation,Defect
R11347 T37922 T37918 prep in,Drives
R11348 T37923 T37924 compound Rb,KO
R11349 T37924 T37925 compound KO,SACs
R11350 T37925 T37922 pobj SACs,in
R11351 T37927 T37928 punct (,A
R11352 T37928 T37929 meta A,diagrams
R11353 T37930 T37928 punct ),A
R11354 T37931 T37929 amod Schematic,diagrams
R11355 T37932 T37929 prep of,diagrams
R11356 T37933 T37934 det the,WT
R11357 T37934 T37932 pobj WT,of
R11358 T37935 T37934 compound mouse,WT
R11359 T37936 T37934 punct ", ",WT
R11360 T37937 T37934 conj E2f3a,WT
R11361 T37938 T37937 punct −,E2f3a
R11362 T37939 T37937 punct /,E2f3a
R11363 T37940 T37937 punct −,E2f3a
R11364 T37941 T37937 punct ", ",E2f3a
R11365 T37942 T37937 cc and,E2f3a
R11366 T37943 T37944 det the,loci
R11367 T37944 T37937 conj loci,E2f3a
R11368 T37945 T37944 nmod Cre,loci
R11369 T37946 T37945 punct -,Cre
R11370 T37947 T37945 amod recombined,Cre
R11371 T37948 T37944 amod floxed,loci
R11372 T37949 T37944 compound E2f3,loci
R11373 T37950 T37944 punct (,loci
R11374 T37951 T37944 acl indicated,loci
R11375 T37952 T37951 advmod here,indicated
R11376 T37953 T37951 prep as,indicated
R11377 T37954 T37953 pobj E2f3,as
R11378 T37955 T37954 punct −,E2f3
R11379 T37956 T37954 punct /,E2f3
R11380 T37957 T37954 punct −,E2f3
R11381 T37958 T37951 prep for,indicated
R11382 T37959 T37958 pobj simplicity,for
R11383 T37960 T37929 punct ),diagrams
R11384 T37961 T37929 punct .,diagrams
R11385 T37963 T37964 nmod E2f3a,mice
R11386 T37964 T37968 nsubj mice,lack
R11387 T37965 T37963 punct −,E2f3a
R11388 T37966 T37963 punct /,E2f3a
R11389 T37967 T37963 punct −,E2f3a
R11390 T37969 T37968 dobj most,lack
R11391 T37970 T37969 prep of,most
R11392 T37971 T37972 compound E2f3,1a
R11393 T37972 T37970 pobj 1a,of
R11394 T37973 T37972 compound exon,1a
R11395 T37974 T37969 cc and,most
R11396 T37975 T37969 conj part,most
R11397 T37976 T37975 prep of,part
R11398 T37977 T37978 compound intron,1a
R11399 T37978 T37976 pobj 1a,of
R11400 T37979 T37980 punct (,box
R11401 T37980 T37968 parataxis box,lack
R11402 T37981 T37980 amod red,box
R11403 T37982 T37980 amod dotted,box
R11404 T37983 T37980 punct ),box
R11405 T37984 T37968 punct .,lack
R11406 T37986 T37987 nsubj Arrows,indicate
R11407 T37988 T37989 compound PCR,primers
R11408 T37989 T37987 dobj primers,indicate
R11409 T37990 T37987 punct .,indicate
R11410 T37992 T37993 nsubjpass Genotyping,shown
R11411 T37994 T37992 prep of,Genotyping
R11412 T37995 T37996 det an,mouse
R11413 T37996 T37994 pobj mouse,of
R11414 T37997 T37996 nmod E2f3a,mouse
R11415 T37998 T37997 punct +,E2f3a
R11416 T37999 T37997 punct /,E2f3a
R11417 T38000 T37997 punct −,E2f3a
R11418 T38001 T37993 auxpass is,shown
R11419 T38002 T37993 prep on,shown
R11420 T38003 T38004 det the,right
R11421 T38004 T38002 pobj right,on
R11422 T38005 T37993 punct .,shown
R11423 T38007 T38008 punct (,B
R11424 T38008 T38009 meta B,detection
R11425 T38010 T38008 punct ),B
R11426 T38011 T38012 compound RT,PCR
R11427 T38012 T38009 compound PCR,detection
R11428 T38013 T38012 punct -,PCR
R11429 T38014 T38009 prep of,detection
R11430 T38015 T38016 nmod E2f3a,mRNA
R11431 T38016 T38014 pobj mRNA,of
R11432 T38017 T38015 cc and,E2f3a
R11433 T38018 T38015 conj E2f3b,E2f3a
R11434 T38019 T38009 prep in,detection
R11435 T38020 T38021 det the,retina
R11436 T38021 T38019 pobj retina,in
R11437 T38022 T38009 punct .,detection
R11438 T38024 T38025 det The,sequences
R11439 T38025 T38026 nsubj sequences,are
R11440 T38027 T38025 prep of,sequences
R11441 T38028 T38027 pobj primers,of
R11442 T38029 T38026 attr 1aF,are
R11443 T38030 T38029 punct (,1aF
R11444 T38031 T38032 nummod 5,GCCTCTACACCACGCCACAAG
R11445 T38032 T38029 appos GCCTCTACACCACGCCACAAG,1aF
R11446 T38033 T38031 punct ′,5
R11447 T38034 T38032 punct -,GCCTCTACACCACGCCACAAG
R11448 T38035 T38032 punct -,GCCTCTACACCACGCCACAAG
R11449 T38036 T38032 nummod 3,GCCTCTACACCACGCCACAAG
R11450 T38037 T38032 punct ′,GCCTCTACACCACGCCACAAG
R11451 T38038 T38029 punct ),1aF
R11452 T38039 T38029 punct ", ",1aF
R11453 T38040 T38029 conj 1bF,1aF
R11454 T38041 T38040 punct (,1bF
R11455 T38042 T38043 nummod 5,CGGAAATGCCCTTACAGC
R11456 T38043 T38040 appos CGGAAATGCCCTTACAGC,1bF
R11457 T38044 T38042 punct ′,5
R11458 T38045 T38043 punct -,CGGAAATGCCCTTACAGC
R11459 T38046 T38043 punct -,CGGAAATGCCCTTACAGC
R11460 T38047 T38043 nummod 3,CGGAAATGCCCTTACAGC
R11461 T38048 T38043 punct ′,CGGAAATGCCCTTACAGC
R11462 T38049 T38040 punct ),1bF
R11463 T38050 T38040 punct ", ",1bF
R11464 T38051 T38040 cc and,1bF
R11465 T38052 T38040 conj 4R,1bF
R11466 T38053 T38052 punct (,4R
R11467 T38054 T38055 nummod 5,CTCAGTCACTTCTTTGGACAG
R11468 T38055 T38052 appos CTCAGTCACTTCTTTGGACAG,4R
R11469 T38056 T38054 punct ′,5
R11470 T38057 T38055 punct -,CTCAGTCACTTCTTTGGACAG
R11471 T38058 T38055 punct -,CTCAGTCACTTCTTTGGACAG
R11472 T38059 T38055 nummod 3,CTCAGTCACTTCTTTGGACAG
R11473 T38060 T38055 punct ′,CTCAGTCACTTCTTTGGACAG
R11474 T38061 T38026 punct ),are
R11475 T38062 T38026 punct .,are
R11476 T38064 T38065 compound WT,retina
R11477 T38065 T38066 nsubj retina,expresses
R11478 T38067 T38068 preconj both,E2f3a
R11479 T38068 T38069 nmod E2f3a,mRNA
R11480 T38069 T38066 dobj mRNA,expresses
R11481 T38070 T38068 cc and,E2f3a
R11482 T38071 T38068 conj E2f3b,E2f3a
R11483 T38072 T38066 punct .,expresses
R11484 T38074 T38075 mark As,expected
R11485 T38075 T38076 advcl expected,lacks
R11486 T38077 T38076 punct ", ",lacks
R11487 T38078 T38079 nmod E2f3a,retina
R11488 T38079 T38076 nsubj retina,lacks
R11489 T38080 T38078 punct −,E2f3a
R11490 T38081 T38078 punct /,E2f3a
R11491 T38082 T38078 punct −,E2f3a
R11492 T38083 T38084 compound E2f3a,mRNA
R11493 T38084 T38076 dobj mRNA,lacks
R11494 T38085 T38076 cc and,lacks
R11495 T38086 T38087 advmod still,expresses
R11496 T38087 T38076 conj expresses,lacks
R11497 T38088 T38089 compound E2f3b,mRNA
R11498 T38089 T38087 dobj mRNA,expresses
R11499 T38090 T38076 punct .,lacks
R11500 T38092 T38093 nmod E2f3,retina
R11501 T38093 T38097 nsubj retina,lacks
R11502 T38094 T38092 punct −,E2f3
R11503 T38095 T38092 punct /,E2f3
R11504 T38096 T38092 punct −,E2f3
R11505 T38098 T38099 amod full,length
R11506 T38099 T38101 nmod length,mRNAs
R11507 T38100 T38099 punct -,length
R11508 T38101 T38097 dobj mRNAs,lacks
R11509 T38102 T38101 nmod E2f3a,mRNAs
R11510 T38103 T38102 cc and,E2f3a
R11511 T38104 T38102 conj E2f3b,E2f3a
R11512 T38105 T38097 punct ", ",lacks
R11513 T38106 T38097 cc and,lacks
R11514 T38107 T38108 advmod instead,expresses
R11515 T38108 T38097 conj expresses,lacks
R11516 T38109 T38110 det a,mRNA
R11517 T38110 T38108 dobj mRNA,expresses
R11518 T38111 T38110 amod truncated,mRNA
R11519 T38112 T38110 acl lacking,mRNA
R11520 T38113 T38112 dobj exon,lacking
R11521 T38114 T38113 nummod 3,exon
R11522 T38115 T38097 punct .,lacks
R11523 T38117 T38118 punct (,C
R11524 T38118 T38119 meta C,analysis
R11525 T38120 T38118 punct ),C
R11526 T38121 T38122 amod Real,time
R11527 T38122 T38119 compound time,analysis
R11528 T38123 T38122 punct -,time
R11529 T38124 T38125 compound RT,PCR
R11530 T38125 T38119 compound PCR,analysis
R11531 T38126 T38125 punct -,PCR
R11532 T38127 T38119 prep of,analysis
R11533 T38128 T38129 compound E2f,genes
R11534 T38129 T38127 pobj genes,of
R11535 T38130 T38119 prep in,analysis
R11536 T38131 T38132 compound P8,retinas
R11537 T38132 T38130 pobj retinas,in
R11538 T38133 T38132 prep of,retinas
R11539 T38134 T38135 det the,genotypes
R11540 T38135 T38133 pobj genotypes,of
R11541 T38136 T38135 amod indicated,genotypes
R11542 T38137 T38119 punct .,analysis
R11543 T38139 T38140 compound Error,bars
R11544 T38140 T38141 nsubj bars,represent
R11545 T38142 T38141 dobj SD,represent
R11546 T38143 T38142 prep of,SD
R11547 T38144 T38143 pobj measurements,of
R11548 T38145 T38144 prep from,measurements
R11549 T38146 T38147 nummod three,animals
R11550 T38147 T38145 pobj animals,from
R11551 T38148 T38141 punct ", ",represent
R11552 T38149 T38141 cc and,represent
R11553 T38150 T38151 nsubj asterisks,indicate
R11554 T38151 T38141 conj indicate,represent
R11555 T38152 T38153 det a,difference
R11556 T38153 T38151 dobj difference,indicate
R11557 T38154 T38153 amod significant,difference
R11558 T38155 T38153 prep between,difference
R11559 T38156 T38155 pobj WT,between
R11560 T38157 T38156 cc and,WT
R11561 T38158 T38159 det the,indicated
R11562 T38159 T38160 nmod indicated,genotypes
R11563 T38160 T38156 conj genotypes,WT
R11564 T38161 T38162 punct (,test
R11565 T38162 T38153 parataxis test,difference
R11566 T38163 T38164 punct *,0.05
R11567 T38164 T38162 ccomp 0.05,test
R11568 T38165 T38164 punct ", ",0.05
R11569 T38166 T38164 nsubj p,0.05
R11570 T38167 T38164 punct <,0.05
R11571 T38168 T38162 punct ;,test
R11572 T38169 T38170 punct **,0.01
R11573 T38170 T38162 ccomp 0.01,test
R11574 T38171 T38170 punct ;,0.01
R11575 T38172 T38170 nsubj p,0.01
R11576 T38173 T38170 punct <,0.01
R11577 T38174 T38162 punct ;,test
R11578 T38175 T38162 nmod ANOVA,test
R11579 T38176 T38175 cc and,ANOVA
R11580 T38177 T38178 compound Tukey,HSD
R11581 T38178 T38175 conj HSD,ANOVA
R11582 T38179 T38162 punct ),test
R11583 T38180 T38151 punct .,indicate
R11584 T38182 T38183 punct (,D
R11585 T38183 T38184 meta D,Rescue
R11586 T38185 T38183 punct ),D
R11587 T38186 T38184 prep of,Rescue
R11588 T38187 T38188 compound Rb,KO
R11589 T38188 T38189 compound KO,SACs
R11590 T38189 T38186 pobj SACs,of
R11591 T38190 T38184 prep by,Rescue
R11592 T38191 T38192 compound E2f3a,deletion
R11593 T38192 T38190 pobj deletion,by
R11594 T38193 T38184 punct .,Rescue
R11595 T38195 T38196 amod Horizontal,sections
R11596 T38196 T38198 nsubjpass sections,stained
R11597 T38197 T38196 amod retinal,sections
R11598 T38199 T38196 prep of,sections
R11599 T38200 T38201 det the,genotypes
R11600 T38201 T38199 pobj genotypes,of
R11601 T38202 T38201 amod indicated,genotypes
R11602 T38203 T38201 cc and,genotypes
R11603 T38204 T38201 conj ages,genotypes
R11604 T38205 T38198 auxpass were,stained
R11605 T38206 T38198 prep for,stained
R11606 T38207 T38206 pobj nuclei,for
R11607 T38208 T38209 punct (,blue
R11608 T38209 T38207 parataxis blue,nuclei
R11609 T38210 T38209 dep DAPI,blue
R11610 T38211 T38209 punct ", ",blue
R11611 T38212 T38209 punct ),blue
R11612 T38213 T38207 punct ", ",nuclei
R11613 T38214 T38215 compound M,phase
R11614 T38215 T38207 conj phase,nuclei
R11615 T38216 T38215 punct -,phase
R11616 T38217 T38218 punct (,green
R11617 T38218 T38215 parataxis green,phase
R11618 T38219 T38218 dep PH3,green
R11619 T38220 T38218 punct ", ",green
R11620 T38221 T38218 punct ),green
R11621 T38222 T38215 punct ", ",phase
R11622 T38223 T38215 cc and,phase
R11623 T38224 T38225 det the,marker
R11624 T38225 T38215 conj marker,phase
R11625 T38226 T38225 compound SAC,marker
R11626 T38227 T38225 appos Slc18a3,marker
R11627 T38228 T38229 punct (,red
R11628 T38229 T38225 parataxis red,marker
R11629 T38230 T38229 punct ),red
R11630 T38231 T38198 punct .,stained
R11631 T38233 T38234 compound E2f3a,deletion
R11632 T38234 T38235 nsubj deletion,suppress
R11633 T38236 T38235 aux does,suppress
R11634 T38237 T38235 neg not,suppress
R11635 T38238 T38239 amod ectopic,division
R11636 T38239 T38235 dobj division,suppress
R11637 T38240 T38235 punct ", ",suppress
R11638 T38241 T38235 cc but,suppress
R11639 T38242 T38235 conj rescues,suppress
R11640 T38243 T38244 det the,defect
R11641 T38244 T38242 dobj defect,rescues
R11642 T38245 T38244 compound SAC,defect
R11643 T38246 T38235 punct .,suppress
R11644 T38248 T38249 compound Scale,bars
R11645 T38249 T38250 nsubj bars,are
R11646 T38251 T38252 nummod 50,μm
R11647 T38252 T38250 attr μm,are
R11648 T38253 T38250 punct .,are
R11649 T38256 T38255 punct ", ",M
R11650 T38257 T38258 amod molecular,size
R11651 T38258 T38259 compound size,marker
R11652 T38259 T38255 appos marker,M
R11653 T38260 T38255 punct .,M
R1519 T6888 T6889 nsubj Rb,Regulates
R1520 T6890 T6889 dobj Division,Regulates
R1521 T6891 T6890 cc and,Division
R1522 T6892 T6890 conj Death,Division
R1523 T6893 T6889 prep through,Regulates
R1524 T6894 T6893 pobj E2f1,through
R1525 T6896 T6897 nsubj We,used
R1526 T6898 T6899 det the,transgene
R1527 T6899 T6897 dobj transgene,used
R1528 T6900 T6901 compound α,Cre
R1529 T6901 T6899 compound Cre,transgene
R1530 T6902 T6901 punct -,Cre
R1531 T6903 T6904 aux to,delete
R1532 T6904 T6897 advcl delete,used
R1533 T6905 T6906 amod floxed,exon
R1534 T6906 T6904 dobj exon,delete
R1535 T6907 T6906 compound Rb,exon
R1536 T6908 T6906 nummod 19,exon
R1537 T6909 T6904 prep at,delete
R1538 T6910 T6911 amod embryonic,day
R1539 T6911 T6909 pobj day,at
R1540 T6912 T6911 punct (,day
R1541 T6913 T6911 appos E,day
R1542 T6914 T6911 punct ),day
R1543 T6915 T6911 nummod 10,day
R1544 T6916 T6904 prep in,delete
R1545 T6917 T6918 amod peripheral,retina
R1546 T6918 T6916 pobj retina,in
R1547 T6919 T6920 punct [,2
R1548 T6920 T6897 parataxis 2,used
R1549 T6921 T6920 punct ],2
R1550 T6922 T6897 punct .,used
R1551 T6924 T6925 nmod RbloxP,loxP
R1552 T6925 T6927 nmod loxP,mice
R1553 T6926 T6925 punct /,loxP
R1554 T6927 T6932 nsubjpass mice,bred
R1555 T6928 T6925 punct ;,loxP
R1556 T6929 T6930 compound α,Cre
R1557 T6930 T6925 appos Cre,loxP
R1558 T6931 T6930 punct -,Cre
R1559 T6933 T6932 auxpass were,bred
R1560 T6934 T6932 prep with,bred
R1561 T6935 T6934 pobj strains,with
R1562 T6936 T6935 acl lacking,strains
R1563 T6937 T6936 dobj E2f1,lacking
R1564 T6938 T6937 cc or,E2f1
R1565 T6939 T6937 conj E2f2,E2f1
R1566 T6940 T6936 prep in,lacking
R1567 T6941 T6942 det the,line
R1568 T6942 T6940 pobj line,in
R1569 T6943 T6942 compound germ,line
R1570 T6944 T6935 punct ", ",strains
R1571 T6945 T6935 cc or,strains
R1572 T6946 T6947 det a,strain
R1573 T6947 T6935 conj strain,strains
R1574 T6948 T6947 acl carrying,strain
R1575 T6949 T6950 det a,allele
R1576 T6950 T6948 dobj allele,carrying
R1577 T6951 T6950 amod floxed,allele
R1578 T6952 T6950 compound E2f3,allele
R1579 T6953 T6954 punct [,5
R1580 T6954 T6932 parataxis 5,bred
R1581 T6955 T6954 punct ],5
R1582 T6956 T6932 punct .,bred
R1583 T6958 T6959 nmod RbloxP,loxP
R1584 T6959 T6961 nmod loxP,mice
R1585 T6960 T6959 punct /,loxP
R1586 T6961 T6980 nsubjpass mice,bred
R1587 T6962 T6959 punct ;,loxP
R1588 T6963 T6959 appos E2f1,loxP
R1589 T6964 T6963 punct +,E2f1
R1590 T6965 T6963 punct /,E2f1
R1591 T6966 T6963 punct −,E2f1
R1592 T6967 T6959 cc and,loxP
R1593 T6968 T6969 compound RbloxP,loxP
R1594 T6969 T6959 conj loxP,loxP
R1595 T6970 T6969 punct /,loxP
R1596 T6971 T6969 punct ;,loxP
R1597 T6972 T6969 appos E2f1,loxP
R1598 T6973 T6972 punct +,E2f1
R1599 T6974 T6972 punct /,E2f1
R1600 T6975 T6972 punct −,E2f1
R1601 T6976 T6959 punct ;,loxP
R1602 T6977 T6978 compound α,Cre
R1603 T6978 T6959 appos Cre,loxP
R1604 T6979 T6978 punct -,Cre
R1605 T6981 T6980 auxpass were,bred
R1606 T6982 T6983 aux to,produce
R1607 T6983 T6980 advcl produce,bred
R1608 T6984 T6985 nmod RbloxP,loxP
R1609 T6985 T6987 nmod loxP,mice
R1610 T6986 T6985 punct /,loxP
R1611 T6987 T6983 dobj mice,produce
R1612 T6988 T6985 punct ;,loxP
R1613 T6989 T6985 appos E2f1,loxP
R1614 T6990 T6989 punct −,E2f1
R1615 T6991 T6989 punct /,E2f1
R1616 T6992 T6989 punct −,E2f1
R1617 T6993 T6985 punct ;,loxP
R1618 T6994 T6995 compound α,Cre
R1619 T6995 T6985 appos Cre,loxP
R1620 T6996 T6995 punct -,Cre
R1621 T6997 T6983 prep at,produce
R1622 T6998 T6999 det a,frequency
R1623 T6999 T6997 pobj frequency,at
R1624 T7000 T6999 prep of,frequency
R1625 T7001 T7002 nummod 1,8
R1626 T7002 T7000 pobj 8,of
R1627 T7003 T7002 punct /,8
R1628 T7004 T6980 cc and,bred
R1629 T7005 T7006 compound littermate,controls
R1630 T7006 T6980 conj controls,bred
R1631 T7007 T7006 prep at,controls
R1632 T7008 T7009 det the,same
R1633 T7009 T7007 pobj same,at
R1634 T7010 T7009 cc or,same
R1635 T7011 T7012 nmod higher,frequency
R1636 T7012 T7009 conj frequency,same
R1637 T7013 T7014 punct (,1
R1638 T7014 T7011 parataxis 1,higher
R1639 T7015 T7016 punct /,4
R1640 T7016 T7014 prep 4,1
R1641 T7017 T7014 punct ),1
R1642 T7018 T7006 punct .,controls
R1643 T7020 T7021 prep For,refer
R1644 T7022 T7020 pobj simplicity,For
R1645 T7023 T7021 nsubj we,refer
R1646 T7024 T7021 aux will,refer
R1647 T7025 T7021 prep to,refer
R1648 T7026 T7027 det the,retina
R1649 T7027 T7025 pobj retina,to
R1650 T7028 T7029 nmod RbloxP,loxP
R1651 T7029 T7027 nmod loxP,retina
R1652 T7030 T7029 punct /,loxP
R1653 T7031 T7029 punct ;,loxP
R1654 T7032 T7029 appos E2f1,loxP
R1655 T7033 T7032 punct −,E2f1
R1656 T7034 T7032 punct /,E2f1
R1657 T7035 T7032 punct −,E2f1
R1658 T7036 T7029 punct ;,loxP
R1659 T7037 T7038 compound α,Cre
R1660 T7038 T7029 appos Cre,loxP
R1661 T7039 T7038 punct -,Cre
R1662 T7040 T7027 amod peripheral,retina
R1663 T7041 T7021 prep as,refer
R1664 T7042 T7043 det the,retina
R1665 T7043 T7041 pobj retina,as
R1666 T7044 T7045 nmod Rb,E2f1
R1667 T7045 T7043 nmod E2f1,retina
R1668 T7046 T7045 punct /,E2f1
R1669 T7047 T7048 amod double,knockout
R1670 T7048 T7045 appos knockout,E2f1
R1671 T7049 T7048 punct (,knockout
R1672 T7050 T7048 appos DKO,knockout
R1673 T7051 T7043 punct ),retina
R1674 T7052 T7021 punct .,refer
R1675 T7054 T7055 amod Similar,strategies
R1676 T7055 T7056 nsubjpass strategies,employed
R1677 T7057 T7056 auxpass were,employed
R1678 T7058 T7056 prep in,employed
R1679 T7059 T7060 det the,case
R1680 T7060 T7058 pobj case,in
R1681 T7061 T7060 prep of,case
R1682 T7062 T7061 pobj E2f2,of
R1683 T7063 T7062 cc or,E2f2
R1684 T7064 T7062 conj E2f3,E2f2
R1685 T7065 T7056 punct .,employed
R1686 T7067 T7068 npadvmod Cre,mediated
R1687 T7068 T7070 amod mediated,excision
R1688 T7069 T7068 punct -,mediated
R1689 T7070 T7071 nsubjpass excision,confirmed
R1690 T7072 T7070 prep of,excision
R1691 T7073 T7074 nmod Rb,alleles
R1692 T7074 T7072 pobj alleles,of
R1693 T7075 T7073 cc and,Rb
R1694 T7076 T7073 conj E2f3,Rb
R1695 T7077 T7070 prep in,excision
R1696 T7078 T7079 det the,retina
R1697 T7079 T7077 pobj retina,in
R1698 T7080 T7071 auxpass was,confirmed
R1699 T7081 T7071 prep by,confirmed
R1700 T7082 T7081 pobj PCR,by
R1701 T7083 T7084 mark as,described
R1702 T7084 T7071 advcl described,confirmed
R1703 T7085 T7084 advmod previously,described
R1704 T7086 T7087 punct [,5
R1705 T7087 T7071 parataxis 5,confirmed
R1706 T7088 T7087 nummod 2,5
R1707 T7089 T7087 punct ",",5
R1708 T7090 T7087 punct ],5
R1709 T7091 T7071 punct .,confirmed
R1710 T7093 T7094 aux To,measure
R1711 T7094 T7095 advcl measure,labelled
R1712 T7096 T7097 amod ectopic,division
R1713 T7097 T7094 dobj division,measure
R1714 T7098 T7097 compound cell,division
R1715 T7099 T7095 punct ", ",labelled
R1716 T7100 T7095 nsubjpass mice,labelled
R1717 T7101 T7095 auxpass were,labelled
R1718 T7102 T7095 dep pulse,labelled
R1719 T7103 T7095 punct -,labelled
R1720 T7104 T7095 prep with,labelled
R1721 T7105 T7104 pobj bromodeoxyuridine,with
R1722 T7106 T7105 punct (,bromodeoxyuridine
R1723 T7107 T7105 appos BrdU,bromodeoxyuridine
R1724 T7108 T7095 punct ),labelled
R1725 T7109 T7110 nummod 2,h
R1726 T7110 T7111 npadvmod h,before
R1727 T7111 T7095 prep before,labelled
R1728 T7112 T7111 pobj sacrifice,before
R1729 T7113 T7095 cc and,labelled
R1730 T7114 T7115 det the,retina
R1731 T7115 T7117 nsubj retina,analyzed
R1732 T7116 T7115 amod peripheral,retina
R1733 T7117 T7095 conj analyzed,labelled
R1734 T7118 T7117 prep for,analyzed
R1735 T7119 T7120 compound BrdU,incorporation
R1736 T7120 T7118 pobj incorporation,for
R1737 T7121 T7117 prep by,analyzed
R1738 T7122 T7121 pobj immunofluorescence,by
R1739 T7123 T7095 punct .,labelled
R1740 T7125 T7126 mark As,reported
R1741 T7126 T7127 advcl reported,exhibited
R1742 T7128 T7126 advmod before,reported
R1743 T7129 T7130 punct [,3
R1744 T7130 T7126 parataxis 3,reported
R1745 T7131 T7130 nummod 2,3
R1746 T7132 T7130 punct ",",3
R1747 T7133 T7130 punct ],3
R1748 T7134 T7127 dep ", ",exhibited
R1749 T7135 T7136 compound Rb,KO
R1750 T7136 T7137 compound KO,retinas
R1751 T7137 T7127 nsubj retinas,exhibited
R1752 T7138 T7139 preconj both,spatial
R1753 T7139 T7140 amod spatial,synthesis
R1754 T7140 T7127 dobj synthesis,exhibited
R1755 T7141 T7139 cc and,spatial
R1756 T7142 T7139 conj temporal,spatial
R1757 T7143 T7140 amod ectopic,synthesis
R1758 T7144 T7140 compound DNA,synthesis
R1759 T7145 T7146 punct (,1C
R1760 T7146 T7127 parataxis 1C,exhibited
R1761 T7147 T7146 compound Figures,1C
R1762 T7148 T7146 cc and,1C
R1763 T7149 T7146 conj S1A,1C
R1764 T7150 T7146 punct ),1C
R1765 T7151 T7127 punct .,exhibited
R1766 T7153 T7154 nsubjpass This,detected
R1767 T7155 T7154 auxpass is,detected
R1768 T7156 T7154 advmod easily,detected
R1769 T7157 T7154 prep at,detected
R1770 T7158 T7157 pobj E14,at
R1771 T7159 T7158 punct ", ",E14
R1772 T7160 T7158 conj E16,E14
R1773 T7161 T7160 punct ", ",E16
R1774 T7162 T7160 cc and,E16
R1775 T7163 T7164 amod postnatal,day
R1776 T7164 T7160 conj day,E16
R1777 T7165 T7164 punct (,day
R1778 T7166 T7164 appos P,day
R1779 T7167 T7164 punct ),day
R1780 T7168 T7164 nummod 0,day
R1781 T7169 T7154 prep in,detected
R1782 T7170 T7171 det the,retina
R1783 T7171 T7169 pobj retina,in
R1784 T7172 T7171 amod inner,retina
R1785 T7173 T7174 advmod where,located
R1786 T7174 T7171 relcl located,retina
R1787 T7175 T7176 amod abnormal,RTCs
R1788 T7176 T7174 nsubjpass RTCs,located
R1789 T7177 T7176 nmod BrdU,RTCs
R1790 T7178 T7177 punct +,BrdU
R1791 T7179 T7176 nmod ganglion,RTCs
R1792 T7180 T7179 cc and,ganglion
R1793 T7181 T7179 conj amacrine,ganglion
R1794 T7182 T7174 auxpass are,located
R1795 T7183 T7169 punct ", ",in
R1796 T7184 T7169 cc or,in
R1797 T7185 T7169 conj on,in
R1798 T7186 T7187 det the,region
R1799 T7187 T7185 pobj region,on
R1800 T7188 T7187 amod outermost,region
R1801 T7189 T7187 prep of,region
R1802 T7190 T7191 det the,retina
R1803 T7191 T7189 pobj retina,of
R1804 T7192 T7191 compound P0,retina
R1805 T7193 T7191 punct ", ",retina
R1806 T7194 T7195 advmod where,reside
R1807 T7195 T7191 relcl reside,retina
R1808 T7196 T7197 nmod BrdU,RTCs
R1809 T7197 T7195 nsubj RTCs,reside
R1810 T7198 T7196 punct +,BrdU
R1811 T7199 T7197 compound photoreceptor,RTCs
R1812 T7200 T7201 punct (,arrows
R1813 T7201 T7154 parataxis arrows,detected
R1814 T7202 T7203 compound Figures,S1A
R1815 T7203 T7201 dep S1A,arrows
R1816 T7204 T7203 cc and,S1A
R1817 T7205 T7203 conj S2,S1A
R1818 T7206 T7201 punct ", ",arrows
R1819 T7207 T7201 punct ),arrows
R1820 T7208 T7209 punct [,2
R1821 T7209 T7154 parataxis 2,detected
R1822 T7210 T7209 punct ],2
R1823 T7211 T7154 punct .,detected
R1824 T7213 T7214 amod Ectopic,division
R1825 T7214 T7216 nsubj division,is
R1826 T7215 T7214 compound RTC,division
R1827 T7217 T7214 prep in,division
R1828 T7218 T7219 compound Rb,KO
R1829 T7219 T7220 compound KO,retinas
R1830 T7220 T7217 pobj retinas,in
R1831 T7221 T7222 advmod even,more
R1832 T7222 T7223 advmod more,obvious
R1833 T7223 T7216 acomp obvious,is
R1834 T7224 T7216 prep at,is
R1835 T7225 T7224 pobj P8,at
R1836 T7226 T7225 cc or,P8
R1837 T7227 T7225 conj P18,P8
R1838 T7228 T7225 punct ", ",P8
R1839 T7229 T7230 advmod when,completed
R1840 T7230 T7225 relcl completed,P8
R1841 T7231 T7230 nsubjpass division,completed
R1842 T7232 T7230 auxpass is,completed
R1843 T7233 T7230 prep in,completed
R1844 T7234 T7235 amod wild,type
R1845 T7235 T7237 nmod type,retina
R1846 T7236 T7235 punct -,type
R1847 T7237 T7233 pobj retina,in
R1848 T7238 T7235 punct (,type
R1849 T7239 T7235 appos WT,type
R1850 T7240 T7237 punct ),retina
R1851 T7241 T7242 punct (,1C
R1852 T7242 T7216 parataxis 1C,is
R1853 T7243 T7242 compound Figures,1C
R1854 T7244 T7242 cc and,1C
R1855 T7245 T7242 conj S1A,1C
R1856 T7246 T7242 punct ),1C
R1857 T7247 T7216 punct .,is
R1858 T7249 T7250 advmod Strikingly,suppressed
R1859 T7251 T7250 punct ", ",suppressed
R1860 T7252 T7253 det the,cells
R1861 T7253 T7250 nsubjpass cells,suppressed
R1862 T7254 T7255 advmod ectopically,positioned
R1863 T7255 T7253 amod positioned,cells
R1864 T7256 T7257 compound S,phase
R1865 T7257 T7253 compound phase,cells
R1866 T7258 T7257 punct -,phase
R1867 T7259 T7253 prep at,cells
R1868 T7260 T7259 pobj E14,at
R1869 T7261 T7260 punct ", ",E14
R1870 T7262 T7260 conj E16,E14
R1871 T7263 T7262 punct ", ",E16
R1872 T7264 T7262 cc and,E16
R1873 T7265 T7262 conj P0,E16
R1874 T7266 T7253 cc and,cells
R1875 T7267 T7268 predet all,division
R1876 T7268 T7253 conj division,cells
R1877 T7269 T7268 det the,division
R1878 T7270 T7268 amod abnormal,division
R1879 T7271 T7268 prep at,division
R1880 T7272 T7271 pobj P8,at
R1881 T7273 T7272 cc and,P8
R1882 T7274 T7272 conj P18,P8
R1883 T7275 T7250 auxpass were,suppressed
R1884 T7276 T7250 advmod completely,suppressed
R1885 T7277 T7250 prep in,suppressed
R1886 T7278 T7279 det the,retina
R1887 T7279 T7277 pobj retina,in
R1888 T7280 T7281 compound Rb,E2f1
R1889 T7281 T7283 compound E2f1,DKO
R1890 T7282 T7281 punct /,E2f1
R1891 T7283 T7279 compound DKO,retina
R1892 T7284 T7285 punct (,1C
R1893 T7285 T7250 parataxis 1C,suppressed
R1894 T7286 T7285 compound Figures,1C
R1895 T7287 T7285 punct ", ",1C
R1896 T7288 T7285 conj 1E,1C
R1897 T7289 T7288 punct ", ",1E
R1898 T7290 T7288 conj 1F,1E
R1899 T7291 T7290 punct ", ",1F
R1900 T7292 T7290 conj S1A,1F
R1901 T7293 T7292 punct ", ",S1A
R1902 T7294 T7292 cc and,S1A
R1903 T7295 T7292 conj S2,S1A
R1904 T7296 T7285 punct ),1C
R1905 T7297 T7250 punct .,suppressed
R1906 T7299 T7300 prep In,had
R1907 T7301 T7299 pobj contrast,In
R1908 T7302 T7300 punct ", ",had
R1909 T7303 T7300 nsubj deletion,had
R1910 T7304 T7303 prep of,deletion
R1911 T7305 T7304 pobj E2f2,of
R1912 T7306 T7305 cc or,E2f2
R1913 T7307 T7305 conj E2f3,E2f2
R1914 T7308 T7309 det no,effect
R1915 T7309 T7300 dobj effect,had
R1916 T7310 T7300 prep at,had
R1917 T7311 T7312 det any,stage
R1918 T7312 T7310 pobj stage,at
R1919 T7313 T7312 prep of,stage
R1920 T7314 T7313 pobj development,of
R1921 T7315 T7300 punct .,had
R1922 T7317 T7318 nsubj Analysis,confirmed
R1923 T7319 T7317 prep of,Analysis
R1924 T7320 T7321 amod mitotic,cells
R1925 T7321 T7319 pobj cells,of
R1926 T7322 T7317 prep with,Analysis
R1927 T7323 T7324 npadvmod anti–phosphohistone,specific
R1928 T7324 T7330 amod specific,antibodies
R1929 T7325 T7323 nummod 3,anti–phosphohistone
R1930 T7326 T7323 punct (,anti–phosphohistone
R1931 T7327 T7323 appos PH3,anti–phosphohistone
R1932 T7328 T7324 punct ),specific
R1933 T7329 T7324 punct –,specific
R1934 T7330 T7322 pobj antibodies,with
R1935 T7331 T7332 mark that,suppressed
R1936 T7332 T7318 ccomp suppressed,confirmed
R1937 T7333 T7332 nsubj loss,suppressed
R1938 T7334 T7333 prep of,loss
R1939 T7335 T7334 pobj E2f1,of
R1940 T7336 T7335 punct ", ",E2f1
R1941 T7337 T7335 cc but,E2f1
R1942 T7338 T7337 neg not,but
R1943 T7339 T7335 conj E2f2,E2f1
R1944 T7340 T7339 cc or,E2f2
R1945 T7341 T7339 conj E2f3,E2f2
R1946 T7342 T7332 punct ", ",suppressed
R1947 T7343 T7344 amod ectopic,division
R1948 T7344 T7332 dobj division,suppressed
R1949 T7345 T7346 punct (,S3
R1950 T7346 T7318 parataxis S3,confirmed
R1951 T7347 T7346 compound Figure,S3
R1952 T7348 T7346 punct ),S3
R1953 T7349 T7318 punct .,confirmed
R1954 T7351 T7352 csubj Deleting,suppressed
R1955 T7353 T7354 nummod one,allele
R1956 T7354 T7351 dobj allele,Deleting
R1957 T7355 T7354 compound E2f1,allele
R1958 T7356 T7352 advmod partially,suppressed
R1959 T7357 T7358 amod ectopic,phase
R1960 T7358 T7352 dobj phase,suppressed
R1961 T7359 T7358 compound S,phase
R1962 T7360 T7358 punct -,phase
R1963 T7361 T7358 cc and,phase
R1964 T7362 T7358 conj mitosis,phase
R1965 T7363 T7352 prep in,suppressed
R1966 T7364 T7365 compound Rb,KO
R1967 T7365 T7366 compound KO,RTCs
R1968 T7366 T7363 pobj RTCs,in
R1969 T7367 T7368 punct (,1C
R1970 T7368 T7352 parataxis 1C,suppressed
R1971 T7369 T7368 compound Figures,1C
R1972 T7370 T7368 punct ", ",1C
R1973 T7371 T7368 conj 1E,1C
R1974 T7372 T7371 punct ", ",1E
R1975 T7373 T7371 conj 1F,1E
R1976 T7374 T7373 punct ", ",1F
R1977 T7375 T7373 conj S1A,1F
R1978 T7376 T7375 punct ", ",S1A
R1979 T7377 T7375 conj S2,S1A
R1980 T7378 T7377 punct ", ",S2
R1981 T7379 T7377 cc and,S2
R1982 T7380 T7377 conj S3,S2
R1983 T7381 T7368 punct ),1C
R1984 T7382 T7352 punct ", ",suppressed
R1985 T7383 T7352 advcl suggesting,suppressed
R1986 T7384 T7385 mark that,drives
R1987 T7385 T7383 ccomp drives,suggesting
R1988 T7386 T7385 nsubj E2f1,drives
R1989 T7387 T7388 amod ectopic,division
R1990 T7388 T7385 dobj division,drives
R1991 T7389 T7385 prep in,drives
R1992 T7390 T7391 compound Rb,KO
R1993 T7391 T7392 compound KO,RTCs
R1994 T7392 T7389 pobj RTCs,in
R1995 T7393 T7385 prep in,drives
R1996 T7394 T7395 det a,fashion
R1997 T7395 T7393 pobj fashion,in
R1998 T7450 T7448 dobj cells,eliminating
R1999 T7396 T7397 npadvmod dose,dependent
R2000 T7451 T7450 amod bipolar,cells
R2001 T7397 T7395 amod dependent,fashion
R2002 T7452 T7451 cc and,bipolar
R2003 T7398 T7397 punct -,dependent
R2004 T7453 T7451 conj ganglion,bipolar
R2005 T7454 T7455 advmod as,as
R2006 T7455 T7450 cc as,cells
R2007 T7399 T7352 punct .,suppressed
R2008 T7456 T7455 advmod well,as
R2009 T7457 T7458 amod many,rods
R2010 T7401 T7402 det These,data
R2011 T7458 T7450 conj rods,cells
R2012 T7459 T7460 punct (,2A
R2013 T7460 T7448 parataxis 2A,eliminating
R2014 T7461 T7460 compound Figure,2A
R2015 T7402 T7403 nsubj data,contrast
R2016 T7462 T7463 punct –,2D
R2017 T7463 T7460 prep 2D,2A
R2018 T7404 T7403 prep with,contrast
R2019 T7464 T7460 punct ),2A
R2020 T7465 T7466 punct [,3
R2021 T7466 T7448 parataxis 3,eliminating
R2022 T7467 T7466 nummod 2,3
R2023 T7405 T7406 amod previous,findings
R2024 T7468 T7466 punct ",",3
R2025 T7469 T7466 punct ],3
R2026 T7406 T7404 pobj findings,with
R2027 T7470 T7437 punct .,results
R2028 T7407 T7403 prep in,contrast
R2029 T7472 T7473 det The,loss
R2030 T7473 T7474 nsubj loss,is
R2031 T7475 T7473 prep of,loss
R2032 T7476 T7477 compound Rb,KO
R2033 T7408 T7409 det the,lens
R2034 T7409 T7407 pobj lens,in
R2035 T7477 T7478 compound KO,rods
R2036 T7410 T7409 cc and,lens
R2037 T7478 T7475 pobj rods,of
R2038 T7479 T7474 acomp evident,is
R2039 T7480 T7474 prep from,is
R2040 T7411 T7409 conj CNS,lens
R2041 T7481 T7482 det the,ONL
R2042 T7412 T7409 prep of,lens
R2043 T7482 T7480 pobj ONL,from
R2044 T7483 T7482 amod thinner,ONL
R2045 T7484 T7474 punct ", ",is
R2046 T7485 T7474 cc and,is
R2047 T7413 T7414 compound Rb,KO
R2048 T7486 T7487 det the,death
R2049 T7487 T7488 nsubjpass death,detected
R2050 T7414 T7415 compound KO,embryos
R2051 T7488 T7474 conj detected,is
R2052 T7489 T7487 prep of,death
R2053 T7490 T7491 det these,cells
R2054 T7415 T7412 pobj embryos,of
R2055 T7491 T7489 pobj cells,of
R2056 T7492 T7493 advmod as,as
R2057 T7416 T7409 punct ", ",lens
R2058 T7493 T7491 cc as,cells
R2059 T7494 T7493 advmod well,as
R2060 T7495 T7496 amod bipolar,neurons
R2061 T7417 T7418 advmod where,suppresses
R2062 T7496 T7491 conj neurons,cells
R2063 T7497 T7495 cc and,bipolar
R2064 T7498 T7495 conj ganglion,bipolar
R2065 T7418 T7409 relcl suppresses,lens
R2066 T7499 T7488 aux can,detected
R2067 T7500 T7488 auxpass be,detected
R2068 T7501 T7488 advmod directly,detected
R2069 T7419 T7418 nsubj deletion,suppresses
R2070 T7502 T7488 prep by,detected
R2071 T7503 T7504 advmod double,labelling
R2072 T7420 T7419 prep of,deletion
R2073 T7504 T7502 pcomp labelling,by
R2074 T7505 T7504 prep for,labelling
R2075 T7506 T7507 amod apoptotic,markers
R2076 T7421 T7422 det any,E2f
R2077 T7507 T7505 pobj markers,for
R2078 T7508 T7506 cc and,apoptotic
R2079 T7422 T7420 pobj E2f,of
R2080 T7509 T7510 compound cell,type
R2081 T7510 T7512 npadvmod type,specific
R2082 T7511 T7510 punct -,type
R2083 T7512 T7506 conj specific,apoptotic
R2084 T7513 T7512 punct -,specific
R2085 T7514 T7515 punct [,2
R2086 T7423 T7422 amod activating,E2f
R2087 T7515 T7488 parataxis 2,detected
R2088 T7516 T7515 punct ],2
R2089 T7517 T7518 punct (,M.
R2090 T7424 T7425 amod ectopic,division
R2091 T7518 T7488 meta M.,detected
R2092 T7519 T7518 nmod P.,M.
R2093 T7425 T7418 dobj division,suppresses
R2094 T7520 T7518 cc and,M.
R2095 T7521 T7518 conj R.,M.
R2096 T7522 T7521 nmod B.,R.
R2097 T7426 T7418 prep to,suppresses
R2098 T7523 T7521 punct ", ",R.
R2099 T7524 T7525 amod unpublished,data
R2100 T7525 T7521 conj data,R.
R2101 T7427 T7428 det some,extent
R2102 T7526 T7525 punct ),data
R2103 T7527 T7488 punct .,detected
R2104 T7428 T7426 pobj extent,to
R2105 T7529 T7530 nsubj Loss,is
R2106 T7429 T7430 punct [,6
R2107 T7531 T7529 prep of,Loss
R2108 T7532 T7533 amod peripheral,cells
R2109 T7533 T7531 pobj cells,of
R2110 T7430 T7418 parataxis 6,suppresses
R2111 T7534 T7535 compound Rb,KO
R2112 T7535 T7533 compound KO,cells
R2113 T7536 T7533 compound ganglion,cells
R2114 T7431 T7432 punct –,9
R2115 T7537 T7530 advmod also,is
R2116 T7538 T7530 acomp evident,is
R2117 T7432 T7430 prep 9,6
R2118 T7539 T7530 prep from,is
R2119 T7540 T7539 pobj thinning,from
R2120 T7541 T7540 prep of,thinning
R2121 T7433 T7430 punct ],6
R2122 T7542 T7543 det the,nerve
R2123 T7543 T7541 pobj nerve,of
R2124 T7544 T7543 amod optic,nerve
R2125 T7434 T7403 punct .,contrast
R2126 T7545 T7546 punct (,D.
R2127 T7546 T7530 meta D.,is
R2128 T7436 T7437 nsubj Loss,results
R2129 T7547 T7546 nmod C.,D.
R2130 T7548 T7546 cc and,D.
R2131 T7549 T7546 conj R.,D.
R2132 T7550 T7549 nmod B.,R.
R2133 T7438 T7436 prep of,Loss
R2134 T7551 T7549 punct ", ",R.
R2135 T7552 T7553 amod unpublished,data
R2136 T7553 T7549 conj data,R.
R2137 T7554 T7553 punct ),data
R2138 T7555 T7530 punct .,is
R2139 T7439 T7438 pobj Rb,of
R2140 T7440 T7436 prep in,Loss
R2141 T7441 T7442 det the,retina
R2142 T7557 T7558 csubj Deleting,blocked
R2143 T7442 T7440 pobj retina,in
R2144 T7559 T7557 dobj E2f1,Deleting
R2145 T7560 T7559 punct ", ",E2f1
R2146 T7443 T7437 prep in,results
R2147 T7561 T7559 cc but,E2f1
R2148 T7444 T7445 amod considerable,apoptosis
R2149 T7445 T7443 pobj apoptosis,in
R2150 T7446 T7445 compound RTC,apoptosis
R2151 T7562 T7561 neg not,but
R2152 T7563 T7559 conj E2f2,E2f1
R2153 T7447 T7437 punct ", ",results
R2154 T7564 T7563 cc or,E2f2
R2155 T7448 T7437 advcl eliminating,results
R2156 T7565 T7563 conj E2f3,E2f2
R2157 T7566 T7558 punct ", ",blocked
R2158 T7567 T7568 det this,death
R2159 T7568 T7558 dobj death,blocked
R2160 T7449 T7450 amod most,cells
R2161 T7569 T7568 amod ectopic,death
R2162 T7570 T7568 compound cell,death
R2163 T7588 T7589 aux To,investigate
R2164 T7571 T7558 prep in,blocked
R2165 T7572 T7573 det a,fashion
R2166 T7573 T7571 pobj fashion,in
R2167 T7574 T7575 npadvmod dose,dependent
R2168 T7575 T7573 amod dependent,fashion
R2169 T7589 T7590 advcl investigate,assessed
R2170 T7576 T7575 punct -,dependent
R2171 T7577 T7578 punct (,1D
R2172 T7591 T7592 det the,mechanism
R2173 T7578 T7558 parataxis 1D,blocked
R2174 T7579 T7578 compound Figures,1D
R2175 T7580 T7578 punct ", ",1D
R2176 T7581 T7578 conj 1G,1D
R2177 T7592 T7589 dobj mechanism,investigate
R2178 T7582 T7581 punct ", ",1G
R2179 T7583 T7581 cc and,1G
R2180 T7584 T7581 conj S1B,1G
R2181 T7593 T7592 amod molecular,mechanism
R2182 T7585 T7578 punct ),1D
R2183 T7586 T7558 punct .,blocked
R2184 T7594 T7595 dep that,underlies
R2185 T7595 T7592 relcl underlies,mechanism
R2186 T7596 T7597 det the,role
R2187 T7597 T7595 dobj role,underlies
R2188 T7598 T7597 amod unique,role
R2189 T7683 T7682 cc and,BrdU
R2190 T7684 T7685 amod terminal,labelling
R2191 T7599 T7597 prep of,role
R2192 T7685 T7682 conj labelling,BrdU
R2193 T7686 T7685 compound dUTP,labelling
R2194 T7600 T7599 pobj E2f1,of
R2195 T7687 T7685 compound nick,labelling
R2196 T7688 T7685 punct -,labelling
R2197 T7689 T7685 compound end,labelling
R2198 T7601 T7590 punct ", ",assessed
R2199 T7690 T7685 punct (,labelling
R2200 T7691 T7685 appos TUNEL,labelling
R2201 T7602 T7590 nsubj we,assessed
R2202 T7692 T7681 punct ),analyses
R2203 T7693 T7678 punct ", ",reversed
R2204 T7603 T7604 det the,expression
R2205 T7694 T7695 compound E2f1,deletion
R2206 T7695 T7678 nsubj deletion,reversed
R2207 T7696 T7678 advmod specifically,reversed
R2208 T7604 T7590 dobj expression,assessed
R2209 T7697 T7698 predet all,defects
R2210 T7605 T7604 prep of,expression
R2211 T7698 T7678 dobj defects,reversed
R2212 T7606 T7607 amod known,targets
R2213 T7699 T7698 det these,defects
R2214 T7607 T7605 pobj targets,of
R2215 T7700 T7698 amod molecular,defects
R2216 T7701 T7678 punct ", ",reversed
R2217 T7702 T7678 cc but,reversed
R2218 T7608 T7607 compound E2f,targets
R2219 T7703 T7704 compound E2f3,deletion
R2220 T7704 T7705 nsubj deletion,had
R2221 T7705 T7678 conj had,reversed
R2222 T7609 T7610 advmod as,as
R2223 T7706 T7707 det no,effect
R2224 T7707 T7705 dobj effect,had
R2225 T7610 T7607 cc as,targets
R2226 T7708 T7709 punct (,1H
R2227 T7709 T7705 parataxis 1H,had
R2228 T7611 T7610 advmod well,as
R2229 T7710 T7709 compound Figure,1H
R2230 T7711 T7709 punct ),1H
R2231 T7712 T7705 punct .,had
R2232 T7612 T7613 amod other,genes
R2233 T7613 T7607 conj genes,targets
R2234 T7614 T7615 dep that,regulate
R2235 T7615 T7613 relcl regulate,genes
R2236 T7616 T7617 det the,cycle
R2237 T7617 T7615 dobj cycle,regulate
R2238 T7618 T7617 compound cell,cycle
R2239 T7619 T7617 cc and,cycle
R2240 T7620 T7617 conj apoptosis,cycle
R2241 T7621 T7590 punct .,assessed
R2242 T7623 T7624 amod Numerous,cycle
R2243 T7624 T7629 nsubjpass cycle,regulated
R2244 T7625 T7624 amod positive,cycle
R2245 T7626 T7625 cc and,positive
R2246 T7627 T7625 conj negative,positive
R2247 T7628 T7624 compound cell,cycle
R2248 T7630 T7624 cc and,cycle
R2249 T7631 T7632 amod apoptotic,regulators
R2250 T7632 T7624 conj regulators,cycle
R2251 T7633 T7629 auxpass were,regulated
R2252 T7634 T7629 advmod up,regulated
R2253 T7635 T7629 punct -,regulated
R2254 T7636 T7629 prep in,regulated
R2255 T7637 T7638 det the,retina
R2256 T7638 T7636 pobj retina,in
R2257 T7639 T7640 compound Rb,KO
R2258 T7640 T7638 compound KO,retina
R2259 T7641 T7642 punct (,1H
R2260 T7642 T7629 parataxis 1H,regulated
R2261 T7643 T7642 compound Figure,1H
R2262 T7644 T7642 punct ),1H
R2263 T7645 T7629 punct .,regulated
R2264 T7647 T7648 prep Among,induced
R2265 T7649 T7650 det the,family
R2266 T7650 T7647 pobj family,Among
R2267 T7651 T7650 compound E2f,family
R2268 T7652 T7648 punct ", ",induced
R2269 T7653 T7648 nsubjpass E2f1,induced
R2270 T7654 T7653 punct ", ",E2f1
R2271 T7655 T7653 conj E2f2,E2f1
R2272 T7656 T7655 punct ", ",E2f2
R2273 T7657 T7655 conj E2f3a,E2f2
R2274 T7658 T7657 punct ", ",E2f3a
R2275 T7659 T7657 cc and,E2f3a
R2276 T7660 T7657 conj E2f7,E2f3a
R2277 T7661 T7648 auxpass were,induced
R2278 T7662 T7648 prep following,induced
R2279 T7663 T7664 compound Rb,loss
R2280 T7664 T7662 pobj loss,following
R2281 T7665 T7648 punct ", ",induced
R2282 T7666 T7648 cc but,induced
R2283 T7667 T7668 nsubj E2f3b,were
R2284 T7668 T7648 conj were,induced
R2285 T7669 T7667 punct ", ",E2f3b
R2286 T7670 T7667 conj E2f4,E2f3b
R2287 T7671 T7670 punct ", ",E2f4
R2288 T7672 T7670 cc and,E2f4
R2289 T7673 T7670 conj E2f5,E2f4
R2290 T7674 T7668 acomp unaffected,were
R2291 T7675 T7668 punct .,were
R2292 T7677 T7678 advcl Consistent,reversed
R2293 T7679 T7677 prep with,Consistent
R2294 T7680 T7681 det the,analyses
R2295 T7681 T7679 pobj analyses,with
R2296 T7682 T7681 nmod BrdU,analyses
R2308 T9110 T9111 amod Normal,Differentiation
R2309 T9112 T9111 prep in,Differentiation
R2310 T9113 T9114 det the,Retina
R2311 T9114 T9112 pobj Retina,in
R2312 T9115 T9116 compound Rb,E2f1
R2313 T9116 T9118 compound E2f1,DKO
R2314 T9117 T9116 punct /,E2f1
R2315 T9118 T9114 compound DKO,Retina
R2316 T9120 T9121 mark Because,blocks
R2317 T9121 T9124 advcl blocks,provided
R2318 T9122 T9123 compound E2f1,deletion
R2319 T9123 T9121 nsubj deletion,blocks
R2320 T9125 T9126 amod abnormal,division
R2321 T9126 T9121 dobj division,blocks
R2322 T9127 T9126 cc and,division
R2323 T9128 T9126 conj death,division
R2324 T9129 T9121 prep in,blocks
R2325 T9130 T9131 det the,retina
R2326 T9131 T9129 pobj retina,in
R2327 T9132 T9133 compound Rb,KO
R2328 T9133 T9131 compound KO,retina
R2329 T9134 T9124 punct ", ",provided
R2330 T9135 T9136 det the,retina
R2331 T9136 T9124 nsubj retina,provided
R2332 T9137 T9138 compound Rb,E2f1
R2333 T9138 T9140 compound E2f1,DKO
R2334 T9139 T9138 punct /,E2f1
R2335 T9140 T9136 compound DKO,retina
R2336 T9141 T9142 det a,opportunity
R2337 T9142 T9124 dobj opportunity,provided
R2338 T9143 T9142 amod unique,opportunity
R2339 T9144 T9145 aux to,evaluate
R2340 T9145 T9142 acl evaluate,opportunity
R2341 T9146 T9147 mark whether,controls
R2342 T9147 T9145 ccomp controls,evaluate
R2343 T9148 T9147 nsubj Rb,controls
R2344 T9149 T9147 dobj differentiation,controls
R2345 T9150 T9147 advcl independent,controls
R2346 T9151 T9150 prep of,independent
R2347 T9152 T9153 compound cell,cycle
R2348 T9153 T9154 compound cycle,effects
R2349 T9154 T9151 pobj effects,of
R2350 T9155 T9124 punct .,provided
R2351 T9157 T9158 det The,retina
R2352 T9158 T9163 nsubj retina,had
R2353 T9159 T9160 compound Rb,E2f1
R2354 T9160 T9162 compound E2f1,DKO
R2355 T9161 T9160 punct /,E2f1
R2356 T9162 T9158 compound DKO,retina
R2357 T9164 T9165 amod many,photoreceptors
R2358 T9165 T9163 dobj photoreceptors,had
R2359 T9166 T9165 nmod Sag,photoreceptors
R2360 T9167 T9166 punct +,Sag
R2361 T9168 T9166 punct (,Sag
R2362 T9169 T9170 compound S,arrestin
R2363 T9170 T9166 appos arrestin,Sag
R2364 T9171 T9170 punct -,arrestin
R2365 T9172 T9170 compound antigen,arrestin
R2366 T9173 T9170 punct /,arrestin
R2367 T9174 T9170 compound rod,arrestin
R2368 T9175 T9165 punct ),photoreceptors
R2369 T9176 T9165 punct ", ",photoreceptors
R2370 T9177 T9178 nmod Pou4f2,cells
R2371 T9178 T9165 conj cells,photoreceptors
R2372 T9179 T9177 punct +,Pou4f2
R2373 T9180 T9177 punct (,Pou4f2
R2374 T9181 T9177 appos Brn3b,Pou4f2
R2375 T9182 T9177 punct ),Pou4f2
R2376 T9183 T9178 compound ganglion,cells
R2377 T9184 T9178 punct ", ",cells
R2378 T9185 T9178 cc and,cells
R2379 T9186 T9187 amod numerous,neurons
R2380 T9187 T9178 conj neurons,cells
R2381 T9188 T9187 nmod Prkca,neurons
R2382 T9189 T9188 punct +,Prkca
R2383 T9190 T9188 punct /,Prkca
R2384 T9191 T9188 appos Cabp5,Prkca
R2385 T9192 T9191 punct +,Cabp5
R2386 T9193 T9187 amod bipolar,neurons
R2387 T9194 T9195 punct (,2A
R2388 T9195 T9163 parataxis 2A,had
R2389 T9196 T9195 compound Figure,2A
R2390 T9197 T9198 punct –,2D
R2391 T9198 T9195 prep 2D,2A
R2392 T9199 T9195 punct ),2A
R2393 T9200 T9163 punct .,had
R2394 T9202 T9203 prep In,was
R2395 T9204 T9202 pobj contrast,In
R2396 T9205 T9203 punct ", ",was
R2397 T9206 T9203 expl there,was
R2398 T9207 T9208 det no,rescue
R2399 T9208 T9203 attr rescue,was
R2400 T9209 T9208 amod such,rescue
R2401 T9210 T9208 prep of,rescue
R2402 T9211 T9212 compound cell,types
R2403 T9212 T9210 pobj types,of
R2404 T9213 T9208 prep in,rescue
R2405 T9214 T9215 nmod Rb,E2f2
R2406 T9215 T9217 nmod E2f2,DKO
R2407 T9216 T9215 punct /,E2f2
R2408 T9217 T9222 compound DKO,retinas
R2409 T9218 T9215 cc or,E2f2
R2410 T9219 T9220 nmod Rb,E2f3
R2411 T9220 T9215 conj E2f3,E2f2
R2412 T9221 T9220 punct /,E2f3
R2413 T9222 T9213 pobj retinas,in
R2414 T9223 T9224 punct (,S4
R2415 T9224 T9203 parataxis S4,was
R2416 T9225 T9224 compound Figure,S4
R2417 T9226 T9224 punct ),S4
R2418 T9227 T9203 punct .,was
R2419 T9229 T9230 nsubj Analysis,confirmed
R2420 T9231 T9229 prep with,Analysis
R2421 T9232 T9233 amod general,Mtap2
R2422 T9233 T9231 pobj Mtap2,with
R2423 T9234 T9233 amod neuronal,Mtap2
R2424 T9235 T9233 compound markers,Mtap2
R2425 T9236 T9233 punct (,Mtap2
R2426 T9237 T9233 appos MAP2,Mtap2
R2427 T9238 T9233 punct ),Mtap2
R2428 T9239 T9233 cc and,Mtap2
R2429 T9240 T9233 conj Snap25,Mtap2
R2430 T9241 T9233 punct ", ",Mtap2
R2431 T9242 T9243 advmod as,as
R2432 T9243 T9233 cc as,Mtap2
R2433 T9244 T9243 advmod well,as
R2434 T9245 T9246 amod other,markers
R2435 T9246 T9233 conj markers,Mtap2
R2436 T9247 T9246 acl expressed,markers
R2437 T9248 T9247 prep in,expressed
R2438 T9249 T9250 amod bipolar,cells
R2439 T9250 T9248 pobj cells,in
R2440 T9251 T9246 punct (,markers
R2441 T9252 T9246 appos Chx10,markers
R2442 T9253 T9252 punct ", ",Chx10
R2443 T9254 T9252 conj Rcvrn,Chx10
R2444 T9255 T9254 punct ", ",Rcvrn
R2445 T9256 T9254 conj Vsx1,Rcvrn
R2446 T9257 T9256 punct ", ",Vsx1
R2447 T9258 T9256 conj Tacr3,Vsx1
R2448 T9259 T9258 punct ", ",Tacr3
R2449 T9260 T9258 cc and,Tacr3
R2450 T9261 T9258 conj Atp2b1,Tacr3
R2451 T9262 T9246 punct ),markers
R2452 T9263 T9246 cc and,markers
R2453 T9264 T9265 compound rod,photoreceptors
R2454 T9265 T9246 conj photoreceptors,markers
R2455 T9266 T9265 punct (,photoreceptors
R2456 T9267 T9265 appos Rho,photoreceptors
R2457 T9268 T9267 cc and,Rho
R2458 T9269 T9267 conj Rcvrn,Rho
R2459 T9270 T9230 punct ),confirmed
R2460 T9271 T9230 dobj rescue,confirmed
R2461 T9272 T9271 prep of,rescue
R2462 T9273 T9274 det the,retina
R2463 T9274 T9272 pobj retina,of
R2464 T9275 T9276 compound Rb,E2f1
R2465 T9276 T9278 compound E2f1,DKO
R2466 T9277 T9276 punct /,E2f1
R2467 T9278 T9274 compound DKO,retina
R2468 T9279 T9280 punct (,S1
R2469 T9280 T9230 parataxis S1,confirmed
R2470 T9281 T9280 compound Table,S1
R2471 T9282 T9280 punct ),S1
R2472 T9283 T9230 punct .,confirmed
R2473 T9285 T9286 advmod Moreover,exhibited
R2474 T9287 T9286 punct ", ",exhibited
R2475 T9288 T9286 nsubj neurons,exhibited
R2476 T9289 T9290 det the,morphology
R2477 T9290 T9286 dobj morphology,exhibited
R2478 T9291 T9290 amod same,morphology
R2479 T9292 T9290 compound complex,morphology
R2480 T9293 T9290 prep as,morphology
R2481 T9294 T9293 prep in,as
R2482 T9295 T9296 compound WT,retina
R2483 T9296 T9294 pobj retina,in
R2484 T9297 T9286 punct .,exhibited
R2485 T9299 T9300 amod Bipolar,bodies
R2486 T9300 T9302 nsubjpass bodies,located
R2487 T9301 T9300 compound cell,bodies
R2488 T9303 T9302 auxpass were,located
R2489 T9304 T9302 prep in,located
R2490 T9305 T9306 det the,INL
R2491 T9306 T9304 pobj INL,in
R2492 T9307 T9302 cc and,located
R2493 T9308 T9302 conj had,located
R2494 T9309 T9310 amod ascending,processes
R2495 T9310 T9308 dobj processes,had
R2496 T9311 T9309 cc and,ascending
R2497 T9312 T9309 conj descending,ascending
R2498 T9313 T9310 acl ending,processes
R2499 T9314 T9313 prep in,ending
R2500 T9315 T9316 det the,OPL
R2501 T9316 T9314 pobj OPL,in
R2502 T9317 T9316 cc and,OPL
R2503 T9318 T9316 conj IPL,OPL
R2504 T9319 T9308 punct ", ",had
R2505 T9320 T9308 advmod respectively,had
R2506 T9321 T9322 punct (,2A
R2507 T9322 T9308 parataxis 2A,had
R2508 T9323 T9322 compound Figure,2A
R2509 T9324 T9322 punct ),2A
R2510 T9325 T9302 punct .,located
R2511 T9327 T9328 prep In,had
R2512 T9329 T9327 pobj addition,In
R2513 T9330 T9328 punct ", ",had
R2514 T9331 T9332 det the,retina
R2515 T9332 T9328 nsubj retina,had
R2516 T9333 T9334 compound Rb,E2f1
R2517 T9334 T9336 compound E2f1,DKO
R2518 T9335 T9334 punct /,E2f1
R2519 T9336 T9332 compound DKO,retina
R2520 T9337 T9338 det a,ONL
R2521 T9338 T9328 dobj ONL,had
R2522 T9339 T9340 amod healthy,appearing
R2523 T9340 T9338 amod appearing,ONL
R2524 T9341 T9338 acl consisting,ONL
R2525 T9342 T9341 prep of,consisting
R2526 T9343 T9344 advmod morphologically,normal
R2527 T9344 T9345 amod normal,rods
R2528 T9345 T9342 pobj rods,of
R2529 T9346 T9328 prep with,had
R2530 T9347 T9348 amod descending,processes
R2531 T9348 T9346 pobj processes,with
R2532 T9349 T9348 acl ending,processes
R2533 T9350 T9349 prep in,ending
R2534 T9351 T9352 det the,OPL
R2535 T9352 T9350 pobj OPL,in
R2536 T9353 T9348 cc and,processes
R2537 T9354 T9355 amod ascending,processes
R2538 T9355 T9348 conj processes,processes
R2539 T9356 T9357 dep that,terminated
R2540 T9357 T9355 relcl terminated,processes
R2541 T9358 T9357 prep in,terminated
R2542 T9359 T9360 amod inner,segments
R2543 T9360 T9358 pobj segments,in
R2544 T9361 T9359 cc and,inner
R2545 T9362 T9359 conj outer,inner
R2546 T9363 T9364 punct (,2A
R2547 T9364 T9328 parataxis 2A,had
R2548 T9365 T9364 compound Figure,2A
R2549 T9366 T9364 punct ),2A
R2550 T9367 T9328 punct .,had
R2551 T9369 T9370 nsubjpass It,suggested
R2552 T9371 T9370 auxpass was,suggested
R2553 T9372 T9373 mark that,regulate
R2554 T9373 T9370 ccomp regulate,suggested
R2555 T9374 T9373 nsubj Rb,regulate
R2556 T9375 T9373 aux might,regulate
R2557 T9376 T9377 compound photoreceptor,differentiation
R2558 T9377 T9373 dobj differentiation,regulate
R2559 T9378 T9373 punct ", ",regulate
R2560 T9379 T9380 advmod possibly,through
R2561 T9380 T9373 prep through,regulate
R2562 T9381 T9382 npadvmod rod,specific
R2563 T9382 T9384 amod specific,factors
R2564 T9383 T9382 punct -,specific
R2565 T9384 T9380 pobj factors,through
R2566 T9385 T9384 compound transcription,factors
R2567 T9386 T9387 punct (,1B
R2568 T9387 T9370 parataxis 1B,suggested
R2569 T9388 T9387 compound Figure,1B
R2570 T9389 T9387 punct ),1B
R2571 T9390 T9391 punct [,29
R2572 T9391 T9370 parataxis 29,suggested
R2573 T9392 T9391 punct ],29
R2574 T9393 T9370 punct .,suggested
R2575 T9395 T9396 advmod However,does
R2576 T9397 T9396 punct ", ",does
R2577 T9398 T9399 mark if,regulate
R2578 T9399 T9396 advcl regulate,does
R2579 T9400 T9399 nsubj Rb,regulate
R2580 T9401 T9399 aux does,regulate
R2581 T9402 T9403 compound photoreceptor,differentiation
R2582 T9403 T9399 dobj differentiation,regulate
R2583 T9404 T9396 punct ", ",does
R2584 T9405 T9396 nsubj it,does
R2585 T9406 T9396 advmod so,does
R2586 T9407 T9396 prep by,does
R2587 T9408 T9407 pcomp inhibiting,by
R2588 T9409 T9408 dobj E2f1,inhibiting
R2589 T9410 T9407 punct ", ",by
R2590 T9411 T9407 cc not,by
R2591 T9412 T9407 conj by,by
R2592 T9413 T9412 pcomp potentiating,by
R2593 T9414 T9415 compound rod,differentiation
R2594 T9415 T9416 compound differentiation,factors
R2595 T9416 T9413 dobj factors,potentiating
R2596 T9417 T9416 punct ", ",factors
R2597 T9418 T9419 amod such,as
R2598 T9419 T9416 prep as,factors
R2599 T9420 T9419 pobj Otx2,as
R2600 T9421 T9420 punct ", ",Otx2
R2601 T9422 T9420 conj Crx,Otx2
R2602 T9423 T9422 punct ", ",Crx
R2603 T9424 T9422 cc or,Crx
R2604 T9425 T9422 conj Nrl,Crx
R2605 T9426 T9396 punct .,does
R2606 T9428 T9429 nsubj It,is
R2607 T9430 T9429 acomp impossible,is
R2608 T9431 T9432 aux to,tell
R2609 T9432 T9429 xcomp tell,is
R2610 T9433 T9434 mark whether,perturbs
R2611 T9434 T9432 ccomp perturbs,tell
R2612 T9435 T9434 nsubj E2f1,perturbs
R2613 T9436 T9434 dobj differentiation,perturbs
R2614 T9437 T9434 advmod directly,perturbs
R2615 T9438 T9434 punct ", ",perturbs
R2616 T9439 T9434 prep by,perturbs
R2617 T9440 T9439 pcomp affecting,by
R2618 T9441 T9442 det the,expression
R2619 T9442 T9440 dobj expression,affecting
R2620 T9443 T9442 prep of,expression
R2621 T9444 T9443 pobj genes,of
R2622 T9445 T9446 dep that,modulate
R2623 T9446 T9444 relcl modulate,genes
R2624 T9447 T9446 dobj maturation,modulate
R2625 T9448 T9434 punct ", ",perturbs
R2626 T9449 T9434 cc and,perturbs
R2627 T9450 T9449 punct /,and
R2628 T9451 T9449 cc or,and
R2629 T9452 T9453 advmod indirectly,1B
R2630 T9453 T9434 conj 1B,perturbs
R2631 T9454 T9453 prep through,1B
R2632 T9455 T9456 poss its,effects
R2633 T9456 T9454 pobj effects,through
R2634 T9457 T9456 prep on,effects
R2635 T9458 T9457 pobj proliferation,on
R2636 T9459 T9458 cc and,proliferation
R2637 T9460 T9458 conj survival,proliferation
R2638 T9461 T9453 punct (,1B
R2639 T9462 T9453 compound Figure,1B
R2640 T9463 T9453 punct ),1B
R2641 T9464 T9429 punct .,is
R2642 T9466 T9467 prep As,was
R2643 T9468 T9466 prep with,As
R2644 T9469 T9470 amod ectopic,division
R2645 T9470 T9468 pobj division,with
R2646 T9471 T9470 cc and,division
R2647 T9472 T9470 conj apoptosis,division
R2648 T9473 T9474 punct (,1C
R2649 T9474 T9470 parataxis 1C,division
R2650 T9475 T9474 compound Figure,1C
R2651 T9476 T9474 cc and,1C
R2652 T9477 T9474 conj 1D,1C
R2653 T9478 T9474 punct ),1C
R2654 T9479 T9467 punct ", ",was
R2655 T9480 T9481 det the,rescue
R2656 T9481 T9467 nsubj rescue,was
R2657 T9482 T9481 prep of,rescue
R2658 T9483 T9484 nmod Rb,KO
R2659 T9484 T9485 nmod KO,cells
R2660 T9485 T9482 pobj cells,of
R2661 T9486 T9485 nmod retinal,cells
R2662 T9487 T9485 amod bipolar,cells
R2663 T9488 T9487 punct ", ",bipolar
R2664 T9489 T9487 conj ganglion,bipolar
R2665 T9490 T9489 punct ", ",ganglion
R2666 T9491 T9489 cc and,ganglion
R2667 T9492 T9489 conj rod,ganglion
R2668 T9493 T9467 acomp dependent,was
R2669 T9494 T9493 prep on,dependent
R2670 T9495 T9496 compound E2f1,dose
R2671 T9496 T9494 pobj dose,on
R2672 T9497 T9498 punct (,2A
R2673 T9498 T9467 parataxis 2A,was
R2674 T9499 T9498 compound Figure,2A
R2675 T9500 T9501 punct –,2D
R2676 T9501 T9498 prep 2D,2A
R2677 T9502 T9498 punct ),2A
R2678 T9503 T9467 punct .,was
R2679 T9505 T9506 advcl Separate,promotes
R2680 T9507 T9505 prep from,Separate
R2681 T9508 T9509 poss its,role
R2682 T9509 T9507 pobj role,from
R2683 T9510 T9509 prep in,role
R2684 T9511 T9510 pcomp driving,in
R2685 T9512 T9513 amod ectopic,division
R2686 T9513 T9511 dobj division,driving
R2687 T9514 T9513 prep of,division
R2688 T9515 T9516 compound Rb,KO
R2689 T9516 T9517 compound KO,RTCs
R2690 T9517 T9514 pobj RTCs,of
R2691 T9518 T9506 punct ", ",promotes
R2692 T9519 T9506 nsubj E2f1,promotes
R2693 T9520 T9506 advmod also,promotes
R2694 T9521 T9522 amod normal,division
R2695 T9522 T9506 dobj division,promotes
R2696 T9523 T9522 compound RPC,division
R2697 T9524 T9525 mark since,drops
R2698 T9525 T9506 advcl drops,promotes
R2699 T9526 T9525 prep in,drops
R2700 T9527 T9528 poss its,absence
R2701 T9528 T9526 pobj absence,in
R2702 T9529 T9530 compound RPC,proliferation
R2703 T9530 T9525 nsubj proliferation,drops
R2704 T9531 T9532 punct ~,2
R2705 T9532 T9525 npadvmod 2,drops
R2706 T9533 T9532 punct -,2
R2707 T9534 T9532 advmod fold,2
R2708 T9535 T9536 punct (,D.
R2709 T9536 T9506 meta D.,promotes
R2710 T9537 T9536 nmod C.,D.
R2711 T9538 T9536 cc and,D.
R2712 T9539 T9536 conj R.,D.
R2713 T9540 T9539 nmod B.,R.
R2714 T9541 T9539 punct ", ",R.
R2715 T9542 T9543 amod unpublished,data
R2716 T9543 T9539 conj data,R.
R2717 T9544 T9543 punct ),data
R2718 T9545 T9506 punct .,promotes
R2719 T9547 T9548 det This,reduction
R2720 T9548 T9550 nsubj reduction,accounts
R2721 T9549 T9548 amod modest,reduction
R2722 T9551 T9548 prep of,reduction
R2723 T9552 T9553 compound RPC,numbers
R2724 T9553 T9551 pobj numbers,of
R2725 T9554 T9548 prep in,reduction
R2726 T9555 T9556 det the,absence
R2727 T9556 T9554 pobj absence,in
R2728 T9557 T9556 prep of,absence
R2729 T9558 T9557 pobj E2f1,of
R2730 T9559 T9550 prep for,accounts
R2731 T9560 T9561 det the,reduction
R2732 T9561 T9559 pobj reduction,for
R2733 T9562 T9561 amod slight,reduction
R2734 T9563 T9561 prep in,reduction
R2735 T9564 T9565 det the,number
R2736 T9565 T9563 pobj number,in
R2737 T9566 T9565 prep of,number
R2738 T9567 T9568 compound ganglion,cells
R2739 T9568 T9566 pobj cells,of
R2740 T9569 T9565 prep at,number
R2741 T9570 T9569 pobj P0,at
R2742 T9571 T9563 punct ", ",in
R2743 T9572 T9563 conj in,in
R2744 T9573 T9574 det the,number
R2745 T9574 T9572 pobj number,in
R2746 T9575 T9574 prep of,number
R2747 T9576 T9577 amod bipolar,cells
R2748 T9577 T9575 pobj cells,of
R2749 T9578 T9574 prep at,number
R2750 T9579 T9578 pobj P18,at
R2751 T9580 T9579 cc or,P18
R2752 T9581 T9579 conj P30,P18
R2753 T9582 T9572 punct ", ",in
R2754 T9583 T9572 cc and,in
R2755 T9584 T9572 conj in,in
R2756 T9585 T9586 det the,thickness
R2757 T9586 T9584 pobj thickness,in
R2758 T9587 T9586 prep of,thickness
R2759 T9588 T9589 det the,ONL
R2760 T9589 T9587 pobj ONL,of
R2761 T9590 T9586 prep at,thickness
R2762 T9591 T9590 pobj P18,at
R2763 T9592 T9591 cc or,P18
R2764 T9593 T9591 conj P30,P18
R2765 T9594 T9586 prep in,thickness
R2766 T9595 T9596 det the,retina
R2767 T9596 T9594 pobj retina,in
R2768 T9597 T9598 nmod E2f1,KO
R2769 T9598 T9596 nmod KO,retina
R2770 T9599 T9598 cc and,KO
R2771 T9600 T9601 compound Rb,E2f1
R2772 T9601 T9603 compound E2f1,DKO
R2773 T9602 T9601 punct /,E2f1
R2774 T9603 T9598 conj DKO,KO
R2775 T9604 T9605 punct (,2B
R2776 T9605 T9550 parataxis 2B,accounts
R2777 T9606 T9605 compound Figure,2B
R2778 T9607 T9608 punct –,2D
R2779 T9608 T9605 prep 2D,2B
R2780 T9609 T9605 punct ),2B
R2781 T9610 T9550 punct .,accounts
R2782 T9612 T9613 det The,morphology
R2783 T9613 T9614 nsubj morphology,was
R2784 T9615 T9613 prep of,morphology
R2785 T9616 T9617 compound E2f1,KO
R2786 T9617 T9618 compound KO,neurons
R2787 T9618 T9615 pobj neurons,of
R2788 T9619 T9614 attr WT,was
R2789 T9620 T9621 punct (,2A
R2790 T9621 T9614 parataxis 2A,was
R2791 T9622 T9621 compound Figure,2A
R2792 T9623 T9621 punct ),2A
R2793 T9624 T9614 punct .,was
R2794 T9626 T9627 prep Despite,was
R2795 T9628 T9629 det a,drop
R2796 T9629 T9626 pobj drop,Despite
R2797 T9630 T9629 amod slight,drop
R2798 T9631 T9629 prep in,drop
R2799 T9632 T9633 amod absolute,numbers
R2800 T9633 T9631 pobj numbers,in
R2801 T9634 T9633 compound cell,numbers
R2802 T9635 T9627 punct ", ",was
R2803 T9636 T9637 det the,proportion
R2804 T9637 T9627 nsubj proportion,was
R2805 T9638 T9637 prep of,proportion
R2806 T9639 T9640 nmod Rb,E2f1
R2807 T9640 T9642 nmod E2f1,DKO
R2808 T9641 T9640 punct /,E2f1
R2809 T9642 T9643 nmod DKO,cells
R2810 T9643 T9638 pobj cells,of
R2811 T9644 T9642 cc and,DKO
R2812 T9645 T9646 compound E2f1,KO
R2813 T9646 T9642 conj KO,DKO
R2814 T9647 T9643 amod bipolar,cells
R2815 T9648 T9649 det the,same
R2816 T9649 T9627 attr same,was
R2817 T9650 T9649 prep as,same
R2818 T9651 T9650 pobj WT,as
R2819 T9652 T9653 punct (,shown
R2820 T9653 T9627 parataxis shown,was
R2821 T9654 T9653 nsubj data,shown
R2822 T9655 T9653 neg not,shown
R2823 T9656 T9653 punct ),shown
R2824 T9657 T9627 punct .,was
R2825 T9659 T9660 advmod Slightly,reduced
R2826 T9660 T9661 amod reduced,numbers
R2827 T9661 T9663 nsubj numbers,were
R2828 T9662 T9661 compound cell,numbers
R2829 T9664 T9663 neg not,were
R2830 T9665 T9663 prep due,were
R2831 T9666 T9665 pcomp to,due
R2832 T9667 T9668 amod residual,death
R2833 T9668 T9665 pobj death,due
R2834 T9669 T9668 compound RTC,death
R2835 T9670 T9671 mark since,observed
R2836 T9671 T9663 advcl observed,were
R2837 T9672 T9671 nsubj we,observed
R2838 T9673 T9671 aux have,observed
R2839 T9674 T9671 neg not,observed
R2840 T9675 T9676 amod ectopic,apoptosis
R2841 T9676 T9671 dobj apoptosis,observed
R2842 T9677 T9671 prep at,observed
R2843 T9678 T9679 det any,stage
R2844 T9679 T9677 pobj stage,at
R2845 T9680 T9679 amod embryonic,stage
R2846 T9681 T9680 cc or,embryonic
R2847 T9682 T9680 conj postnatal,embryonic
R2848 T9683 T9671 prep in,observed
R2849 T9684 T9685 det the,retina
R2850 T9685 T9683 pobj retina,in
R2851 T9686 T9685 amod developing,retina
R2852 T9687 T9688 compound Rb,E2f1
R2853 T9688 T9690 compound E2f1,DKO
R2854 T9689 T9688 punct /,E2f1
R2855 T9690 T9685 compound DKO,retina
R2856 T9691 T9692 punct (,1D
R2857 T9692 T9671 parataxis 1D,observed
R2858 T9693 T9692 compound Figures,1D
R2859 T9694 T9692 punct ", ",1D
R2860 T9695 T9692 conj 1G,1D
R2861 T9696 T9695 punct ", ",1G
R2862 T9697 T9695 cc and,1G
R2863 T9698 T9695 conj S2,1G
R2864 T9699 T9692 punct ),1D
R2865 T9700 T9663 punct .,were
R2866 T9702 T9703 advmod Moreover,reduces
R2867 T9704 T9703 punct ", ",reduces
R2868 T9705 T9703 csubj deleting,reduces
R2869 T9706 T9705 dobj Ccnd1,deleting
R2870 T9707 T9706 punct ", ",Ccnd1
R2871 T9708 T9709 dep which,acts
R2872 T9709 T9706 relcl acts,Ccnd1
R2873 T9710 T9709 advmod upstream,acts
R2874 T9711 T9710 prep of,upstream
R2875 T9712 T9713 compound Rb,proteins
R2876 T9713 T9711 pobj proteins,of
R2877 T9714 T9703 punct ", ",reduces
R2878 T9715 T9703 advmod also,reduces
R2879 T9716 T9717 compound RPC,number
R2880 T9717 T9703 dobj number,reduces
R2881 T9718 T9703 punct ", ",reduces
R2882 T9719 T9703 cc but,reduces
R2883 T9720 T9721 aux does,suppress
R2884 T9721 T9703 conj suppress,reduces
R2885 T9722 T9721 neg not,suppress
R2886 T9723 T9724 det any,defect
R2887 T9724 T9721 dobj defect,suppress
R2888 T9725 T9724 prep in,defect
R2889 T9726 T9727 det the,retina
R2890 T9727 T9725 pobj retina,in
R2891 T9728 T9729 compound Rb,KO
R2892 T9729 T9727 compound KO,retina
R2893 T9730 T9731 punct (,D.
R2894 T9731 T9721 meta D.,suppress
R2895 T9732 T9731 nmod C.,D.
R2896 T9733 T9731 cc and,D.
R2897 T9734 T9731 conj R.,D.
R2898 T9735 T9734 nmod B.,R.
R2899 T9736 T9734 punct ", ",R.
R2900 T9737 T9738 amod unpublished,data
R2901 T9738 T9734 conj data,R.
R2902 T9739 T9738 punct ),data
R2903 T9740 T9703 punct .,reduces
R2904 T9742 T9743 advmod Thus,are
R2905 T9744 T9743 punct ", ",are
R2906 T9745 T9746 advmod slightly,reduced
R2907 T9746 T9747 amod reduced,division
R2908 T9747 T9743 nsubj division,are
R2909 T9748 T9747 compound RPC,division
R2910 T9749 T9747 cc and,division
R2911 T9750 T9751 amod dramatic,rescue
R2912 T9751 T9747 conj rescue,division
R2913 T9752 T9751 prep of,rescue
R2914 T9753 T9754 amod severe,defects
R2915 T9754 T9752 pobj defects,of
R2916 T9755 T9751 prep in,rescue
R2917 T9756 T9757 compound Rb,KO
R2918 T9757 T9758 compound KO,RTCs
R2919 T9758 T9755 pobj RTCs,in
R2920 T9759 T9760 amod distinct,effects
R2921 T9760 T9743 attr effects,are
R2922 T9761 T9760 acl stemming,effects
R2923 T9762 T9761 prep from,stemming
R2924 T9763 T9764 det the,deletion
R2925 T9764 T9762 pobj deletion,from
R2926 T9765 T9764 prep of,deletion
R2927 T9766 T9765 pobj E2f1,of
R2928 T9767 T9743 punct .,are
R2937 T11029 T11030 amod Normal,Function
R2938 T11031 T11030 prep of,Function
R2939 T11032 T11033 det the,Retina
R2940 T11033 T11031 pobj Retina,of
R2941 T11034 T11035 compound Rb,E2f1
R2942 T11035 T11037 compound E2f1,DKO
R2943 T11036 T11035 punct /,E2f1
R2944 T11037 T11033 compound DKO,Retina
R2945 T11039 T11040 det The,discovery
R2946 T11040 T11041 nsubj discovery,is
R2947 T11042 T11043 mark that,rescues
R2948 T11043 T11040 acl rescues,discovery
R2949 T11044 T11045 compound E2f1,loss
R2950 T11045 T11043 nsubj loss,rescues
R2951 T11046 T11047 advmod even,morphology
R2952 T11047 T11043 dobj morphology,rescues
R2953 T11048 T11047 det the,morphology
R2954 T11049 T11047 prep of,morphology
R2955 T11050 T11051 compound Rb,KO
R2956 T11051 T11052 compound KO,neurons
R2957 T11052 T11049 pobj neurons,of
R2958 T11053 T11041 acomp surprising,is
R2959 T11054 T11055 mark because,thought
R2960 T11055 T11041 advcl thought,is
R2961 T11056 T11055 nsubjpass Rb,thought
R2962 T11057 T11055 auxpass is,thought
R2963 T11058 T11059 aux to,regulate
R2964 T11059 T11055 xcomp regulate,thought
R2965 T11060 T11059 dobj differentiation,regulate
R2966 T11061 T11062 advmod primarily,through
R2967 T11062 T11059 prep through,regulate
R2968 T11063 T11064 npadvmod E2f,independent
R2969 T11064 T11066 amod independent,pathways
R2970 T11065 T11064 punct -,independent
R2971 T11066 T11062 pobj pathways,through
R2972 T11067 T11068 punct [,30
R2973 T11068 T11041 parataxis 30,is
R2974 T11069 T11070 punct –,33
R2975 T11070 T11068 prep 33,30
R2976 T11071 T11068 punct ],30
R2977 T11072 T11041 punct .,is
R2978 T11074 T11075 advmod However,equate
R2979 T11076 T11075 punct ", ",equate
R2980 T11077 T11078 amod normal,morphology
R2981 T11078 T11075 nsubj morphology,equate
R2982 T11079 T11075 aux may,equate
R2983 T11080 T11075 neg not,equate
R2984 T11081 T11075 prep to,equate
R2985 T11082 T11083 advmod completely,normal
R2986 T11083 T11084 amod normal,differentiation
R2987 T11084 T11081 pobj differentiation,to
R2988 T11085 T11075 punct .,equate
R2989 T11087 T11088 advmod Thus,compared
R2990 T11089 T11088 punct ", ",compared
R2991 T11090 T11088 nsubj we,compared
R2992 T11091 T11092 det the,electroretinograms
R2993 T11092 T11088 dobj electroretinograms,compared
R2994 T11093 T11092 punct (,electroretinograms
R2995 T11094 T11092 appos ERGs,electroretinograms
R2996 T11095 T11092 punct ),electroretinograms
R2997 T11096 T11092 prep of,electroretinograms
R2998 T11097 T11098 amod adult,mice
R2999 T11098 T11096 pobj mice,of
R3000 T11099 T11098 nmod WT,mice
R3001 T11100 T11099 punct (,WT
R3002 T11101 T11102 compound α,Cre
R3003 T11102 T11099 appos Cre,WT
R3004 T11103 T11102 punct -,Cre
R3005 T11104 T11099 punct ),WT
R3006 T11105 T11099 punct ", ",WT
R3007 T11106 T11099 conj E2f1,WT
R3008 T11107 T11106 punct −,E2f1
R3009 T11108 T11106 punct /,E2f1
R3010 T11109 T11106 punct −,E2f1
R3011 T11110 T11106 punct ", ",E2f1
R3012 T11111 T11112 compound α,Cre
R3013 T11112 T11106 conj Cre,E2f1
R3014 T11113 T11112 punct -,Cre
R3015 T11114 T11112 punct ;,Cre
R3016 T11115 T11116 compound RbloxP,loxP
R3017 T11116 T11112 appos loxP,Cre
R3018 T11117 T11116 punct /,loxP
R3019 T11118 T11112 punct ", ",Cre
R3020 T11119 T11112 cc and,Cre
R3021 T11120 T11121 compound α,Cre
R3022 T11121 T11112 conj Cre,Cre
R3023 T11122 T11121 punct -,Cre
R3024 T11123 T11121 punct ;,Cre
R3025 T11124 T11125 compound RbloxP,loxP
R3026 T11125 T11121 appos loxP,Cre
R3027 T11126 T11125 punct /,loxP
R3028 T11127 T11121 punct ;,Cre
R3029 T11128 T11121 appos E2f1,Cre
R3030 T11129 T11128 punct −,E2f1
R3031 T11130 T11128 punct /,E2f1
R3032 T11131 T11128 punct −,E2f1
R3033 T11132 T11088 punct .,compared
R3034 T11134 T11135 nsubj ERGs,assess
R3035 T11136 T11135 advmod functionally,assess
R3036 T11137 T11138 amod visual,signalling
R3037 T11138 T11135 dobj signalling,assess
R3038 T11139 T11138 prep in,signalling
R3039 T11140 T11141 det the,retina
R3040 T11141 T11139 pobj retina,in
R3041 T11142 T11141 amod mammalian,retina
R3042 T11143 T11138 prep from,signalling
R3043 T11144 T11143 pobj photoreceptors,from
R3044 T11145 T11143 prep to,from
R3045 T11146 T11147 amod amacrine,cells
R3046 T11147 T11145 pobj cells,to
R3047 T11148 T11147 punct (,cells
R3048 T11149 T11147 cc but,cells
R3049 T11150 T11151 advmod usually,not
R3050 T11151 T11152 neg not,cells
R3051 T11152 T11147 conj cells,cells
R3052 T11153 T11152 compound gangion,cells
R3053 T11154 T11135 punct ),assess
R3054 T11155 T11135 punct ", ",assess
R3055 T11156 T11135 cc and,assess
R3056 T11157 T11158 auxpass are,dominated
R3057 T11158 T11135 conj dominated,assess
R3058 T11159 T11158 agent by,dominated
R3059 T11160 T11161 nmod rod,cells
R3060 T11161 T11159 pobj cells,by
R3061 T11162 T11160 cc and,rod
R3062 T11163 T11160 conj cone,rod
R3063 T11164 T11161 amod bipolar,cells
R3064 T11165 T11135 punct .,assess
R3065 T11167 T11168 advmod Typically,begins
R3066 T11169 T11168 punct ", ",begins
R3067 T11170 T11171 det an,signal
R3068 T11171 T11168 nsubj signal,begins
R3069 T11172 T11171 compound ERG,signal
R3070 T11173 T11168 prep with,begins
R3071 T11174 T11175 det a,deflection
R3072 T11175 T11173 pobj deflection,with
R3073 T11176 T11175 amod negative,deflection
R3074 T11177 T11175 acl initiated,deflection
R3075 T11178 T11177 agent by,initiated
R3076 T11179 T11180 det the,photoreceptors
R3077 T11180 T11178 pobj photoreceptors,by
R3078 T11181 T11175 punct (,deflection
R3079 T11182 T11183 det the,wave
R3080 T11183 T11175 appos wave,deflection
R3081 T11184 T11183 compound a,wave
R3082 T11185 T11183 punct -,wave
R3083 T11186 T11175 punct ),deflection
R3084 T11187 T11175 punct ", ",deflection
R3085 T11188 T11189 dep which,terminated
R3086 T11189 T11175 relcl terminated,deflection
R3087 T11190 T11189 auxpass is,terminated
R3088 T11191 T11189 agent by,terminated
R3089 T11192 T11193 det a,deflection
R3090 T11193 T11191 pobj deflection,by
R3091 T11194 T11193 amod large,deflection
R3092 T11195 T11193 amod positive,deflection
R3093 T11196 T11189 prep due,terminated
R3094 T11197 T11196 pcomp to,due
R3095 T11198 T11199 det the,activation
R3096 T11199 T11196 pobj activation,due
R3097 T11200 T11199 prep of,activation
R3098 T11201 T11202 nmod ON,cells
R3099 T11202 T11200 pobj cells,of
R3100 T11203 T11202 amod bipolar,cells
R3101 T11204 T11189 punct (,terminated
R3102 T11205 T11206 det the,wave
R3103 T11206 T11189 dobj wave,terminated
R3104 T11207 T11206 compound b,wave
R3105 T11208 T11206 punct -,wave
R3106 T11209 T11168 punct ),begins
R3107 T11210 T11168 punct .,begins
R3108 T11212 T11213 nsubj Responses,assess
R3109 T11214 T11212 prep to,Responses
R3110 T11215 T11216 amod dim,light
R3111 T11216 T11214 pobj light,to
R3112 T11217 T11212 prep in,Responses
R3113 T11218 T11219 npadvmod dark,adapted
R3114 T11219 T11221 amod adapted,conditions
R3115 T11220 T11219 punct -,adapted
R3116 T11221 T11217 pobj conditions,in
R3117 T11222 T11223 punct (,scotopic
R3118 T11223 T11219 parataxis scotopic,adapted
R3119 T11224 T11223 punct ),scotopic
R3120 T11225 T11213 advmod specifically,assess
R3121 T11226 T11227 det the,system
R3122 T11227 T11213 dobj system,assess
R3123 T11228 T11227 compound rod,system
R3124 T11229 T11213 punct ", ",assess
R3125 T11230 T11213 cc and,assess
R3126 T11231 T11213 conj were,assess
R3127 T11232 T11231 acomp defective,were
R3128 T11233 T11231 prep in,were
R3129 T11234 T11235 det the,retina
R3130 T11235 T11233 pobj retina,in
R3131 T11236 T11237 compound Rb,KO
R3132 T11237 T11235 compound KO,retina
R3133 T11238 T11239 punct (,2E
R3134 T11239 T11231 parataxis 2E,were
R3135 T11240 T11239 compound Figure,2E
R3136 T11241 T11239 punct ),2E
R3137 T11242 T11213 punct .,assess
R3138 T11244 T11245 det The,reduction
R3139 T11245 T11247 nsubj reduction,is
R3140 T11246 T11245 amod substantial,reduction
R3141 T11248 T11245 prep of,reduction
R3142 T11249 T11250 preconj both,a
R3143 T11250 T11251 nmod a,waves
R3144 T11251 T11248 pobj waves,of
R3145 T11252 T11250 punct -,a
R3146 T11253 T11250 cc and,a
R3147 T11254 T11250 conj b,a
R3148 T11255 T11251 punct -,waves
R3149 T11256 T11247 acomp consistent,is
R3150 T11257 T11256 prep with,consistent
R3151 T11258 T11259 nmod rod,apoptosis
R3152 T11259 T11257 pobj apoptosis,with
R3153 T11260 T11258 cc and,rod
R3154 T11261 T11262 amod bipolar,cell
R3155 T11262 T11258 conj cell,rod
R3156 T11263 T11264 punct [,2
R3157 T11264 T11247 parataxis 2,is
R3158 T11265 T11264 punct ],2
R3159 T11266 T11247 punct .,is
R3160 T11268 T11269 det The,sensitivity
R3161 T11269 T11270 nsubj sensitivity,appeared
R3162 T11271 T11269 prep of,sensitivity
R3163 T11272 T11273 det the,response
R3164 T11273 T11271 pobj response,of
R3165 T11274 T11273 amod residual,response
R3166 T11275 T11270 oprd unchanged,appeared
R3167 T11276 T11270 punct ", ",appeared
R3168 T11277 T11270 advcl suggesting,appeared
R3169 T11278 T11279 nsubj it,arose
R3170 T11279 T11277 advcl arose,suggesting
R3171 T11280 T11279 prep from,arose
R3172 T11281 T11282 det the,portions
R3173 T11282 T11280 pobj portions,from
R3174 T11283 T11284 npadvmod Cre,negative
R3175 T11284 T11282 amod negative,portions
R3176 T11285 T11284 punct -,negative
R3177 T11286 T11282 prep of,portions
R3178 T11287 T11288 det the,retina
R3179 T11288 T11286 pobj retina,of
R3180 T11289 T11270 punct .,appeared
R3181 T11291 T11292 nsubj Responses,were
R3182 T11293 T11294 advmod about,same
R3183 T11294 T11292 attr same,were
R3184 T11295 T11294 det the,same
R3185 T11296 T11292 prep in,were
R3186 T11297 T11298 det the,retina
R3187 T11298 T11296 pobj retina,in
R3188 T11299 T11298 nmod WT,retina
R3189 T11300 T11299 cc and,WT
R3190 T11301 T11302 compound E2f1,KO
R3191 T11302 T11299 conj KO,WT
R3192 T11303 T11292 punct ", ",were
R3193 T11304 T11292 cc and,were
R3194 T11305 T11292 punct ", ",were
R3195 T11306 T11307 advmod most,importantly
R3196 T11307 T11308 advmod importantly,lay
R3197 T11308 T11292 conj lay,were
R3198 T11309 T11308 punct ", ",lay
R3199 T11310 T11308 advmod also,lay
R3200 T11311 T11312 det the,response
R3201 T11312 T11308 nsubj response,lay
R3202 T11313 T11314 compound Rb,E2f1
R3203 T11314 T11316 compound E2f1,DKO
R3204 T11315 T11314 punct /,E2f1
R3205 T11316 T11312 compound DKO,response
R3206 T11317 T11312 amod median,response
R3207 T11318 T11308 prep at,lay
R3208 T11319 T11320 det the,end
R3209 T11320 T11318 pobj end,at
R3210 T11321 T11320 amod lower,end
R3211 T11322 T11320 prep of,end
R3212 T11323 T11324 det the,range
R3213 T11324 T11322 pobj range,of
R3214 T11325 T11324 amod normal,range
R3215 T11326 T11308 prep for,lay
R3216 T11327 T11328 amod most,intensities
R3217 T11328 T11326 pobj intensities,for
R3218 T11329 T11330 punct (,2F
R3219 T11330 T11308 parataxis 2F,lay
R3220 T11331 T11330 compound Figure,2F
R3221 T11332 T11330 punct ),2F
R3222 T11333 T11308 punct .,lay
R3223 T11335 T11336 advmod Thus,rescued
R3224 T11337 T11336 punct ", ",rescued
R3225 T11338 T11339 compound E2f1,deletion
R3226 T11339 T11336 nsubj deletion,rescued
R3227 T11340 T11341 advmod almost,completely
R3228 T11341 T11336 advmod completely,rescued
R3229 T11342 T11343 det the,system
R3230 T11343 T11336 dobj system,rescued
R3231 T11344 T11343 compound rod,system
R3232 T11345 T11336 prep in,rescued
R3233 T11346 T11347 det the,retina
R3234 T11347 T11345 pobj retina,in
R3235 T11348 T11349 compound Rb,KO
R3236 T11349 T11347 compound KO,retina
R3237 T11350 T11336 punct .,rescued
R3238 T11352 T11353 npadvmod Light,adapted
R3239 T11353 T11355 amod adapted,recordings
R3240 T11354 T11353 punct -,adapted
R3241 T11355 T11359 nsubj recordings,yielded
R3242 T11356 T11357 punct (,photopic
R3243 T11357 T11353 parataxis photopic,adapted
R3244 T11358 T11357 punct ),photopic
R3245 T11360 T11361 aux to,assess
R3246 T11361 T11355 advcl assess,recordings
R3247 T11362 T11361 advmod specifically,assess
R3248 T11363 T11364 det the,system
R3249 T11364 T11361 dobj system,assess
R3250 T11365 T11364 compound cone,system
R3251 T11366 T11367 amod comparable,results
R3252 T11367 T11359 dobj results,yielded
R3253 T11368 T11359 punct .,yielded
R3254 T11370 T11371 nsubj Cones,represent
R3255 T11372 T11373 advmod only,3
R3256 T11373 T11374 nummod 3,%
R3257 T11374 T11371 dobj %,represent
R3258 T11375 T11374 prep of,%
R3259 T11376 T11375 pobj photoreceptors,of
R3260 T11377 T11371 cc and,represent
R3261 T11378 T11371 punct ", ",represent
R3262 T11379 T11380 prep unlike,develop
R3263 T11380 T11371 conj develop,represent
R3264 T11381 T11379 pobj rods,unlike
R3265 T11382 T11380 punct ", ",develop
R3266 T11383 T11380 prep without,develop
R3267 T11384 T11383 pobj Rb,without
R3268 T11385 T11371 punct ", ",represent
R3269 T11386 T11371 cc but,represent
R3270 T11387 T11388 nsubj they,require
R3271 T11388 T11371 conj require,represent
R3272 T11389 T11388 dobj rods,require
R3273 T11390 T11388 prep for,require
R3274 T11391 T11390 pobj survival,for
R3275 T11392 T11388 punct ", ",require
R3276 T11393 T11388 cc and,require
R3277 T11394 T11395 prep in,have
R3278 T11395 T11388 conj have,require
R3279 T11396 T11397 det the,retina
R3280 T11397 T11394 pobj retina,in
R3281 T11398 T11399 compound Rb,KO
R3282 T11399 T11397 compound KO,retina
R3283 T11400 T11395 punct ", ",have
R3284 T11401 T11395 nsubj they,have
R3285 T11402 T11403 amod abnormal,morphology
R3286 T11403 T11395 dobj morphology,have
R3287 T11404 T11395 cc and,have
R3288 T11405 T11406 poss their,targets
R3289 T11406 T11408 nsubj targets,are
R3290 T11407 T11406 amod synaptic,targets
R3291 T11408 T11395 conj are,have
R3292 T11409 T11406 punct ", ",targets
R3293 T11410 T11411 amod bipolar,cells
R3294 T11411 T11406 appos cells,targets
R3295 T11412 T11408 punct ", ",are
R3296 T11413 T11414 advmod much,depleted
R3297 T11414 T11408 acomp depleted,are
R3298 T11415 T11416 punct [,2
R3299 T11416 T11408 parataxis 2,are
R3300 T11417 T11416 punct ],2
R3301 T11418 T11408 punct .,are
R3302 T11420 T11421 det The,response
R3303 T11421 T11423 nsubjpass response,reduced
R3304 T11422 T11421 amod photopic,response
R3305 T11424 T11421 punct ", ",response
R3306 T11425 T11426 det a,product
R3307 T11426 T11421 appos product,response
R3308 T11427 T11426 prep of,product
R3309 T11428 T11429 nmod cone,activity
R3310 T11429 T11427 pobj activity,of
R3311 T11430 T11428 cc and,cone
R3312 T11431 T11432 advmod mainly,bipolar
R3313 T11432 T11428 conj bipolar,cone
R3314 T11433 T11423 punct ", ",reduced
R3315 T11434 T11423 auxpass was,reduced
R3316 T11435 T11423 advmod much,reduced
R3317 T11436 T11423 agent by,reduced
R3318 T11437 T11438 compound Rb,loss
R3319 T11438 T11436 pobj loss,by
R3320 T11439 T11423 punct ", ",reduced
R3321 T11440 T11423 cc but,reduced
R3322 T11441 T11442 auxpass was,rescued
R3323 T11442 T11423 conj rescued,reduced
R3324 T11443 T11442 advmod considerably,rescued
R3325 T11444 T11442 prep in,rescued
R3326 T11445 T11446 det the,retina
R3327 T11446 T11444 pobj retina,in
R3328 T11447 T11448 compound Rb,E2f1
R3329 T11448 T11450 compound E2f1,DKO
R3330 T11449 T11448 punct /,E2f1
R3331 T11450 T11446 compound DKO,retina
R3332 T11451 T11452 punct (,S5
R3333 T11452 T11442 parataxis S5,rescued
R3334 T11453 T11452 compound Figure,S5
R3335 T11454 T11452 punct ),S5
R3336 T11455 T11423 punct .,reduced
R3337 T11457 T11458 advmod Again,lay
R3338 T11459 T11458 punct ", ",lay
R3339 T11460 T11461 det the,amplitude
R3340 T11461 T11458 nsubj amplitude,lay
R3341 T11462 T11461 amod median,amplitude
R3342 T11463 T11458 prep at,lay
R3343 T11464 T11465 det the,end
R3344 T11465 T11463 pobj end,at
R3345 T11466 T11465 amod lower,end
R3346 T11467 T11465 prep of,end
R3347 T11468 T11469 det the,range
R3348 T11469 T11467 pobj range,of
R3349 T11470 T11471 compound E2f1,KO
R3350 T11471 T11469 compound KO,range
R3351 T11472 T11458 punct .,lay
R3352 T11474 T11475 det The,response
R3353 T11475 T11477 nsubjpass response,reduced
R3354 T11476 T11475 amod photopic,response
R3355 T11478 T11475 prep in,response
R3356 T11479 T11480 compound E2f1,KO
R3357 T11480 T11481 compound KO,mice
R3358 T11481 T11478 pobj mice,in
R3359 T11482 T11477 auxpass was,reduced
R3360 T11483 T11477 advmod slightly,reduced
R3361 T11484 T11477 advcl relative,reduced
R3362 T11485 T11484 prep to,relative
R3363 T11486 T11485 pobj WT,to
R3364 T11487 T11488 punct (,S5B
R3365 T11488 T11477 parataxis S5B,reduced
R3366 T11489 T11488 compound Figure,S5B
R3367 T11490 T11488 punct ),S5B
R3368 T11491 T11477 punct ", ",reduced
R3369 T11492 T11493 advmod possibly,required
R3370 T11493 T11477 advcl required,reduced
R3371 T11494 T11493 mark because,required
R3372 T11495 T11493 nsubjpass E2f1,required
R3373 T11496 T11493 auxpass is,required
R3374 T11497 T11493 prep for,required
R3375 T11498 T11499 amod maximal,expansion
R3376 T11499 T11497 pobj expansion,for
R3377 T11500 T11499 prep of,expansion
R3378 T11501 T11502 amod embryonic,RPCs
R3379 T11502 T11500 pobj RPCs,of
R3380 T11503 T11477 punct ", ",reduced
R3381 T11504 T11477 cc and,reduced
R3382 T11505 T11506 det the,retina
R3383 T11506 T11509 nsubj retina,has
R3384 T11507 T11508 compound E2f1,KO
R3385 T11508 T11506 compound KO,retina
R3386 T11509 T11477 conj has,reduced
R3387 T11510 T11509 punct ", ",has
R3388 T11511 T11512 mark as,noted
R3389 T11512 T11509 advcl noted,has
R3390 T11513 T11512 advmod earlier,noted
R3391 T11514 T11509 punct ", ",has
R3392 T11515 T11516 advmod slightly,fewer
R3393 T11516 T11517 amod fewer,cells
R3394 T11517 T11509 dobj cells,has
R3395 T11518 T11517 prep than,cells
R3396 T11519 T11520 det the,retina
R3397 T11520 T11518 pobj retina,than
R3398 T11521 T11520 compound WT,retina
R3399 T11522 T11509 punct ", ",has
R3400 T11523 T11524 mark although,are
R3401 T11524 T11509 advcl are,has
R3402 T11525 T11526 compound cell,type
R3403 T11526 T11527 compound type,proportions
R3404 T11527 T11524 nsubj proportions,are
R3405 T11528 T11524 acomp unaffected,are
R3406 T11529 T11530 punct (,D.
R3407 T11530 T11509 meta D.,has
R3408 T11531 T11530 nmod C.,D.
R3409 T11532 T11530 cc and,D.
R3410 T11533 T11530 conj R.,D.
R3411 T11534 T11533 nmod B.,R.
R3412 T11535 T11533 punct ", ",R.
R3413 T11536 T11537 amod unpublished,data
R3414 T11537 T11533 conj data,R.
R3415 T11538 T11537 punct ),data
R3416 T11539 T11509 punct .,has
R3417 T11541 T11542 advmod Thus,attributed
R3418 T11543 T11542 punct ", ",attributed
R3419 T11544 T11545 advmod marginally,subnormal
R3420 T11545 T11546 amod subnormal,responses
R3421 T11546 T11542 nsubjpass responses,attributed
R3422 T11547 T11546 amod photopic,responses
R3423 T11548 T11546 prep in,responses
R3424 T11549 T11550 det the,retina
R3425 T11550 T11548 pobj retina,in
R3426 T11551 T11552 compound Rb,E2f1
R3427 T11552 T11554 compound E2f1,DKO
R3428 T11553 T11552 punct /,E2f1
R3429 T11554 T11550 compound DKO,retina
R3430 T11555 T11542 aux can,attributed
R3431 T11556 T11542 auxpass be,attributed
R3432 T11557 T11542 prep to,attributed
R3433 T11558 T11559 preconj both,reduction
R3434 T11559 T11557 pobj reduction,to
R3435 T11560 T11559 det a,reduction
R3436 T11561 T11559 prep of,reduction
R3437 T11562 T11563 compound cone,numbers
R3438 T11563 T11561 pobj numbers,of
R3439 T11564 T11559 prep in,reduction
R3440 T11565 T11566 compound E2f1,KO
R3441 T11566 T11567 compound KO,mice
R3442 T11567 T11564 pobj mice,in
R3443 T11568 T11567 advmod alone,mice
R3444 T11569 T11559 punct ", ",reduction
R3445 T11570 T11559 cc and,reduction
R3446 T11571 T11572 det a,reduction
R3447 T11572 T11559 conj reduction,reduction
R3448 T11573 T11572 punct “,reduction
R3449 T11574 T11572 amod genuine,reduction
R3450 T11575 T11572 punct ”,reduction
R3451 T11576 T11572 amod slight,reduction
R3452 T11577 T11572 prep in,reduction
R3453 T11578 T11579 compound cone,function
R3454 T11579 T11577 pobj function,in
R3455 T11580 T11572 amod attributable,reduction
R3456 T11581 T11580 prep to,attributable
R3457 T11582 T11583 compound Rb,loss
R3458 T11583 T11581 pobj loss,to
R3459 T11584 T11572 amod relative,reduction
R3460 T11585 T11584 prep to,relative
R3461 T11586 T11585 pobj WT,to
R3462 T11587 T11542 punct .,attributed
R3463 T11589 T11590 det This,effect
R3464 T11590 T11592 nsubj effect,relate
R3465 T11591 T11590 amod slight,effect
R3466 T11593 T11592 aux may,relate
R3467 T11594 T11592 prep to,relate
R3468 T11595 T11596 det a,defect
R3469 T11596 T11594 pobj defect,to
R3470 T11597 T11596 amod true,defect
R3471 T11598 T11596 compound differentiation,defect
R3472 T11599 T11592 prep in,relate
R3473 T11600 T11601 det a,subset
R3474 T11601 T11599 pobj subset,in
R3475 T11602 T11601 prep of,subset
R3476 T11603 T11604 amod amacrine,cells
R3477 T11604 T11602 pobj cells,of
R3478 T11605 T11601 acl discussed,subset
R3479 T11606 T11605 advmod below,discussed
R3480 T11607 T11592 punct .,relate
R3481 T11609 T11610 det This,discussion
R3482 T11610 T11611 nsubj discussion,obscure
R3483 T11612 T11611 aux should,obscure
R3484 T11613 T11611 neg not,obscure
R3485 T11614 T11615 det the,outcome
R3486 T11615 T11611 dobj outcome,obscure
R3487 T11616 T11615 amod major,outcome
R3488 T11617 T11618 mark that,recovers
R3489 T11618 T11615 acl recovers,outcome
R3490 T11619 T11620 compound E2f1,deletion
R3491 T11620 T11618 nsubj deletion,recovers
R3492 T11621 T11618 dobj most,recovers
R3493 T11622 T11621 prep of,most
R3494 T11623 T11624 det the,response
R3495 T11624 T11622 pobj response,of
R3496 T11625 T11624 compound ERG,response
R3497 T11626 T11611 punct .,obscure
R3498 T11628 T11629 advmod Thus,deletion
R3499 T11630 T11629 punct ", ",deletion
R3500 T11631 T11629 compound E2f1,deletion
R3501 T11632 T11629 preconj not,deletion
R3502 T11633 T11632 advmod only,not
R3503 T11634 T11629 conj rescues,deletion
R3504 T11635 T11634 dobj morphology,rescues
R3505 T11636 T11635 cc but,morphology
R3506 T11637 T11636 advmod also,but
R3507 T11638 T11639 preconj both,rod
R3508 T11639 T11640 nmod rod,function
R3509 T11640 T11635 conj function,morphology
R3510 T11641 T11639 cc and,rod
R3511 T11642 T11639 conj cone,rod
R3512 T11643 T11640 compound system,function
R3513 T11644 T11634 prep in,rescues
R3514 T11645 T11646 det the,retina
R3515 T11646 T11644 pobj retina,in
R3516 T11647 T11648 compound Rb,KO
R3517 T11648 T11646 compound KO,retina
R3518 T11649 T11634 punct .,rescues
R3525 T13755 T13756 amod Abnormal,Differentiation
R3526 T13757 T13756 compound SAC,Differentiation
R3527 T13758 T13756 amod Independent,Differentiation
R3528 T13759 T13758 prep of,Independent
R3529 T13760 T13761 compound Cell,Cycle
R3530 T13761 T13759 pobj Cycle,of
R3531 T13762 T13761 cc and,Cycle
R3532 T13763 T13764 compound Survival,Defects
R3533 T13764 T13761 conj Defects,Cycle
R3534 T13766 T13767 nsubj ERGs,assess
R3535 T13768 T13767 advmod primarily,assess
R3536 T13769 T13770 nmod photoreceptor,function
R3537 T13770 T13767 dobj function,assess
R3538 T13771 T13769 cc and,photoreceptor
R3539 T13772 T13773 amod bipolar,cell
R3540 T13773 T13769 conj cell,photoreceptor
R3541 T13774 T13767 punct ", ",assess
R3542 T13775 T13767 cc but,assess
R3543 T13776 T13777 aux may,miss
R3544 T13777 T13767 conj miss,assess
R3545 T13778 T13779 compound differentiation,defects
R3546 T13779 T13777 dobj defects,miss
R3547 T13780 T13777 prep in,miss
R3548 T13781 T13782 amod other,cells
R3549 T13782 T13780 pobj cells,in
R3550 T13783 T13767 punct .,assess
R3551 T13785 T13786 aux To,test
R3552 T13786 T13787 advcl test,stained
R3553 T13788 T13786 prep for,test
R3554 T13789 T13790 amod subtle,differences
R3555 T13790 T13788 pobj differences,for
R3556 T13791 T13787 nsubj we,stained
R3557 T13792 T13793 det the,retina
R3558 T13793 T13787 dobj retina,stained
R3559 T13794 T13795 compound Rb,E2f1
R3560 T13795 T13797 compound E2f1,DKO
R3561 T13796 T13795 punct /,E2f1
R3562 T13797 T13793 compound DKO,retina
R3563 T13798 T13787 prep with,stained
R3564 T13799 T13800 nummod 43,markers
R3565 T13800 T13798 pobj markers,with
R3566 T13801 T13802 punct (,S1
R3567 T13802 T13787 parataxis S1,stained
R3568 T13803 T13802 compound Table,S1
R3569 T13804 T13802 punct ),S1
R3570 T13805 T13787 punct .,stained
R3571 T13807 T13808 compound Thirty,two
R3572 T13808 T13810 nummod two,proteins
R3573 T13809 T13808 punct -,two
R3574 T13810 T13811 nsubj proteins,displayed
R3575 T13812 T13813 amod identical,patterns
R3576 T13813 T13811 dobj patterns,displayed
R3577 T13814 T13811 prep in,displayed
R3578 T13815 T13816 nmod WT,retina
R3579 T13816 T13814 pobj retina,in
R3580 T13817 T13815 punct ", ",WT
R3581 T13818 T13819 compound E2f1,KO
R3582 T13819 T13815 conj KO,WT
R3583 T13820 T13819 punct ", ",KO
R3584 T13821 T13819 cc and,KO
R3585 T13822 T13823 compound Rb,E2f1
R3586 T13823 T13825 compound E2f1,DKO
R3587 T13824 T13823 punct /,E2f1
R3588 T13825 T13819 conj DKO,KO
R3589 T13826 T13827 punct (,S1
R3590 T13827 T13811 parataxis S1,displayed
R3591 T13828 T13827 compound Table,S1
R3592 T13829 T13827 punct ),S1
R3593 T13830 T13811 punct .,displayed
R3594 T13832 T13833 det The,markers
R3595 T13833 T13836 nsubj markers,revealed
R3596 T13834 T13833 amod other,markers
R3597 T13835 T13833 nummod 11,markers
R3598 T13837 T13838 det a,defect
R3599 T13838 T13836 dobj defect,revealed
R3600 T13839 T13840 compound cell,cycle
R3601 T13840 T13842 npadvmod cycle,independent
R3602 T13841 T13840 punct -,cycle
R3603 T13842 T13838 amod independent,defect
R3604 T13843 T13840 punct –,cycle
R3605 T13844 T13840 cc and,cycle
R3606 T13845 T13840 conj apoptosis,cycle
R3607 T13846 T13842 punct -,independent
R3608 T13847 T13838 compound differentiation,defect
R3609 T13848 T13836 prep in,revealed
R3610 T13849 T13848 pobj SACs,in
R3611 T13850 T13836 punct .,revealed
R3612 T13852 T13853 nsubj We,studied
R3613 T13854 T13853 advmod first,studied
R3614 T13855 T13853 dobj Calb2,studied
R3615 T13856 T13855 punct (,Calb2
R3616 T13857 T13855 appos calretinin,Calb2
R3617 T13858 T13855 punct ),Calb2
R3618 T13859 T13855 punct ", ",Calb2
R3619 T13860 T13861 dep which,marks
R3620 T13861 T13855 relcl marks,Calb2
R3621 T13862 T13863 det a,subset
R3622 T13863 T13861 dobj subset,marks
R3623 T13864 T13863 prep of,subset
R3624 T13865 T13866 amod amacrine,bodies
R3625 T13866 T13864 pobj bodies,of
R3626 T13867 T13865 cc and,amacrine
R3627 T13868 T13869 compound ganglion,cell
R3628 T13869 T13865 conj cell,amacrine
R3629 T13870 T13871 advmod as,as
R3630 T13871 T13863 cc as,subset
R3631 T13872 T13871 advmod well,as
R3632 T13873 T13874 nummod three,tracks
R3633 T13874 T13863 conj tracks,subset
R3634 T13875 T13874 acl corresponding,tracks
R3635 T13876 T13875 prep to,corresponding
R3636 T13877 T13878 poss their,processes
R3637 T13878 T13876 pobj processes,to
R3638 T13879 T13878 prep in,processes
R3639 T13880 T13881 det the,IPL
R3640 T13881 T13879 pobj IPL,in
R3641 T13882 T13883 punct (,3A
R3642 T13883 T13861 parataxis 3A,marks
R3643 T13884 T13883 compound Figure,3A
R3644 T13885 T13883 punct ),3A
R3645 T13886 T13853 punct .,studied
R3646 T13888 T13889 amod Normal,staining
R3647 T13889 T13891 nsubjpass staining,seen
R3648 T13890 T13889 compound Calb2,staining
R3649 T13892 T13891 auxpass was,seen
R3650 T13893 T13891 prep in,seen
R3651 T13894 T13895 det the,IPL
R3652 T13895 T13893 pobj IPL,in
R3653 T13896 T13897 compound E2f1,KO
R3654 T13897 T13895 compound KO,IPL
R3655 T13898 T13899 punct (,shown
R3656 T13899 T13891 parataxis shown,seen
R3657 T13900 T13899 nsubj data,shown
R3658 T13901 T13899 neg not,shown
R3659 T13902 T13899 punct ),shown
R3660 T13903 T13891 punct .,seen
R3661 T13905 T13906 advmod However,was
R3662 T13907 T13906 punct ", ",was
R3663 T13908 T13909 advmod only,track
R3664 T13909 T13906 nsubj track,was
R3665 T13910 T13909 nummod one,track
R3666 T13911 T13909 nmod Calb2,track
R3667 T13912 T13911 punct +,Calb2
R3668 T13913 T13906 acomp evident,was
R3669 T13914 T13906 prep in,was
R3670 T13915 T13916 det the,IPL
R3671 T13916 T13914 pobj IPL,in
R3672 T13917 T13918 compound Rb,KO
R3673 T13918 T13916 compound KO,IPL
R3674 T13919 T13906 punct ", ",was
R3675 T13920 T13906 cc and,was
R3676 T13921 T13922 det this,defect
R3677 T13922 T13923 nsubjpass defect,rescued
R3678 T13923 T13906 conj rescued,was
R3679 T13924 T13923 auxpass was,rescued
R3680 T13925 T13923 neg not,rescued
R3681 T13926 T13923 prep in,rescued
R3682 T13927 T13928 det the,retina
R3683 T13928 T13926 pobj retina,in
R3684 T13929 T13930 compound Rb,E2f1
R3685 T13930 T13932 compound E2f1,DKO
R3686 T13931 T13930 punct /,E2f1
R3687 T13932 T13928 compound DKO,retina
R3688 T13933 T13934 punct (,3A
R3689 T13934 T13923 parataxis 3A,rescued
R3690 T13935 T13934 compound Figure,3A
R3691 T13936 T13934 punct ),3A
R3692 T13937 T13923 punct .,rescued
R3693 T13939 T13940 nsubj We,quantified
R3694 T13941 T13942 nmod Calb2,bodies
R3695 T13942 T13940 dobj bodies,quantified
R3696 T13943 T13941 punct +,Calb2
R3697 T13944 T13942 compound cell,bodies
R3698 T13945 T13940 prep in,quantified
R3699 T13946 T13947 det the,INL
R3700 T13947 T13945 pobj INL,in
R3701 T13948 T13949 compound Rb,KO
R3702 T13949 T13947 compound KO,INL
R3703 T13950 T13947 punct (,INL
R3704 T13951 T13947 acl corresponding,INL
R3705 T13952 T13951 prep to,corresponding
R3706 T13953 T13954 amod amacrine,cell
R3707 T13954 T13955 compound cell,staining
R3708 T13955 T13952 pobj staining,to
R3709 T13956 T13955 advmod only,staining
R3710 T13957 T13940 punct ),quantified
R3711 T13958 T13940 cc and,quantified
R3712 T13959 T13940 conj observed,quantified
R3713 T13960 T13961 det a,reduction
R3714 T13961 T13959 dobj reduction,observed
R3715 T13962 T13959 prep from,observed
R3716 T13963 T13962 pobj P8,from
R3717 T13964 T13963 advmod onwards,P8
R3718 T13965 T13966 punct (,3C
R3719 T13966 T13959 parataxis 3C,observed
R3720 T13967 T13966 compound Figures,3C
R3721 T13968 T13966 cc and,3C
R3722 T13969 T13966 conj S6,3C
R3723 T13970 T13966 punct ),3C
R3724 T13971 T13940 punct .,quantified
R3725 T13977 T13976 pobj t,f
R3726 T13980 T13979 amod h,e
R3727 T13987 T13986 advmod l,Ca
R3728 T13990 T13989 punct +,b2
R3729 T13993 T13994 compound r,a
R3730 T13996 T13995 parataxis s,c
R3731 T14004 T14001 punct ", ",are
R3732 T14005 T14006 det the,tracks
R3733 T14006 T14001 nsubj tracks,are
R3734 T14007 T14006 nummod two,tracks
R3735 T14008 T14006 amod outer,tracks
R3736 T14009 T14001 prep from,are
R3737 T14010 T14009 pobj SACs,from
R3738 T14011 T14010 punct ", ",SACs
R3739 T14012 T14010 acl named,SACs
R3740 T14013 T14012 prep after,named
R3741 T14014 T14015 poss their,morphology
R3742 T14015 T14013 pobj morphology,after
R3743 T14016 T14015 amod extensive,morphology
R3744 T14017 T14018 amod dendritic,tree
R3745 T14018 T14020 npadvmod tree,like
R3746 T14019 T14018 punct -,tree
R3747 T14020 T14015 amod like,morphology
R3748 T14021 T14020 punct -,like
R3749 T14022 T14023 punct [,37
R3750 T14023 T14001 parataxis 37,are
R3751 T14024 T14023 punct ],37
R3752 T14025 T14001 punct .,are
R3753 T14027 T14028 nsubj SACs,are
R3754 T14029 T14028 acomp cholinergic,are
R3755 T14030 T14028 punct ", ",are
R3756 T14031 T14028 conj represent,are
R3757 T14032 T14033 punct ~,5.2
R3758 T14033 T14034 nummod 5.2,%
R3759 T14034 T14031 dobj %,represent
R3760 T14035 T14034 prep of,%
R3761 T14036 T14037 amod amacrine,neurons
R3762 T14037 T14035 pobj neurons,of
R3763 T14038 T14039 punct [,38
R3764 T14039 T14031 parataxis 38,represent
R3765 T14040 T14039 punct ],38
R3766 T14041 T14031 punct ", ",represent
R3767 T14042 T14031 cc and,represent
R3768 T14043 T14031 conj are,represent
R3769 T14044 T14043 acomp critical,are
R3770 T14045 T14044 prep for,critical
R3771 T14046 T14047 preconj both,selectivity
R3772 T14047 T14045 pobj selectivity,for
R3773 T14048 T14047 compound direction,selectivity
R3774 T14049 T14050 punct [,35
R3775 T14050 T14047 parataxis 35,selectivity
R3776 T14051 T14050 nummod 34,35
R3777 T14052 T14050 punct ",",35
R3778 T14053 T14050 punct ],35
R3779 T14054 T14047 cc and,selectivity
R3780 T14055 T14056 amod spontaneous,activity
R3781 T14056 T14047 conj activity,selectivity
R3782 T14057 T14056 amod rhythmic,activity
R3783 T14058 T14059 dep that,occurs
R3784 T14059 T14056 relcl occurs,activity
R3785 T14060 T14059 prep during,occurs
R3786 T14061 T14062 amod normal,development
R3787 T14062 T14060 pobj development,during
R3788 T14063 T14062 amod retinal,development
R3789 T14064 T14065 punct [,36
R3790 T14065 T14056 parataxis 36,activity
R3791 T14066 T14065 punct ],36
R3792 T14067 T14028 punct .,are
R3793 T14069 T14070 nsubj SACs,synapse
R3794 T14071 T14069 prep in,SACs
R3795 T14072 T14073 det the,INL
R3796 T14073 T14071 pobj INL,in
R3797 T14074 T14070 prep in,synapse
R3798 T14075 T14076 det the,layer
R3799 T14076 T14074 pobj layer,in
R3800 T14077 T14076 compound OFF,layer
R3801 T14078 T14076 prep of,layer
R3802 T14079 T14080 det the,IPL
R3803 T14080 T14078 pobj IPL,of
R3804 T14081 T14082 dep that,responds
R3805 T14082 T14076 relcl responds,layer
R3806 T14083 T14082 prep to,responds
R3807 T14084 T14085 amod decreasing,light
R3808 T14085 T14083 pobj light,to
R3809 T14086 T14070 punct ", ",synapse
R3810 T14087 T14088 mark while,have
R3811 T14088 T14070 advcl have,synapse
R3812 T14089 T14090 amod displaced,SACs
R3813 T14090 T14088 nsubj SACs,have
R3814 T14091 T14090 prep in,SACs
R3815 T14092 T14093 det the,GCL
R3816 T14093 T14091 pobj GCL,in
R3817 T14094 T14088 dobj processes,have
R3818 T14095 T14096 dep that,synapse
R3819 T14096 T14094 relcl synapse,processes
R3820 T14097 T14096 prep in,synapse
R3821 T14098 T14099 det the,layer
R3822 T14099 T14097 pobj layer,in
R3823 T14100 T14099 amod nearby,layer
R3824 T14101 T14099 compound ON,layer
R3825 T14102 T14099 prep of,layer
R3826 T14103 T14104 det the,IPL
R3827 T14104 T14102 pobj IPL,of
R3828 T14105 T14106 dep that,responds
R3829 T14106 T14099 relcl responds,layer
R3830 T14107 T14106 prep to,responds
R3831 T14108 T14109 amod increasing,light
R3832 T14109 T14107 pobj light,to
R3833 T14110 T14111 punct (,reviewed
R3834 T14111 T14088 parataxis reviewed,have
R3835 T14112 T14111 prep in,reviewed
R3836 T14113 T14112 punct [,in
R3837 T14114 T14112 pobj 39,in
R3838 T14115 T14111 punct ],reviewed
R3839 T14116 T14111 punct ),reviewed
R3840 T14117 T14070 punct .,synapse
R3841 T14119 T14120 amod Mature,processes
R3842 T14120 T14122 nsubj processes,stain
R3843 T14121 T14120 compound SAC,processes
R3844 T14123 T14124 advmod specifically,for
R3845 T14124 T14122 prep for,stain
R3846 T14125 T14124 pobj Slc18a3,for
R3847 T14126 T14125 punct (,Slc18a3
R3848 T14127 T14128 amod vesicular,transporter
R3849 T14128 T14125 appos transporter,Slc18a3
R3850 T14129 T14128 compound acetyl,transporter
R3851 T14130 T14128 compound choline,transporter
R3852 T14131 T14128 punct ", ",transporter
R3853 T14132 T14128 appos VAChT,transporter
R3854 T14133 T14122 punct ),stain
R3855 T14134 T14135 punct [,37
R3856 T14135 T14122 parataxis 37,stain
R3857 T14136 T14135 punct ],37
R3858 T14137 T14122 punct ", ",stain
R3859 T14138 T14122 cc and,stain
R3860 T14139 T14122 punct ", ",stain
R3861 T14140 T14141 advmod significantly,was
R3862 T14141 T14122 conj was,stain
R3863 T14142 T14141 punct ", ",was
R3864 T14143 T14144 det this,marker
R3865 T14144 T14141 nsubj marker,was
R3866 T14145 T14141 acomp absent,was
R3867 T14146 T14141 prep in,was
R3868 T14147 T14148 det the,retina
R3869 T14148 T14146 pobj retina,in
R3870 T14149 T14148 amod peripheral,retina
R3871 T14150 T14151 nmod Rb,KO
R3872 T14151 T14148 nmod KO,retina
R3873 T14152 T14151 cc or,KO
R3874 T14153 T14154 compound Rb,E2f1
R3875 T14154 T14156 compound E2f1,DKO
R3876 T14155 T14154 punct /,E2f1
R3877 T14156 T14151 conj DKO,KO
R3878 T14157 T14148 compound P18,retina
R3879 T14158 T14159 punct (,3A
R3880 T14159 T14141 parataxis 3A,was
R3881 T14160 T14159 compound Figures,3A
R3882 T14161 T14159 cc and,3A
R3883 T14162 T14159 conj S7B,3A
R3884 T14163 T14159 punct ),3A
R3885 T14164 T14141 punct .,was
R3886 T14166 T14167 nsubj Chat,is
R3887 T14168 T14166 punct ", ",Chat
R3888 T14169 T14166 acl expressed,Chat
R3889 T14170 T14169 prep from,expressed
R3890 T14171 T14172 det the,locus
R3891 T14172 T14170 pobj locus,from
R3892 T14173 T14172 amod same,locus
R3893 T14174 T14167 punct ", ",is
R3894 T14175 T14167 advmod also,is
R3895 T14176 T14177 npadvmod SAC,specific
R3896 T14177 T14167 acomp specific,is
R3897 T14178 T14167 punct ", ",is
R3898 T14179 T14167 cc but,is
R3899 T14180 T14167 conj marks,is
R3900 T14181 T14182 preconj both,bodies
R3901 T14182 T14180 dobj bodies,marks
R3902 T14183 T14182 compound cell,bodies
R3903 T14184 T14182 cc and,bodies
R3904 T14185 T14182 conj processes,bodies
R3905 T14186 T14182 prep of,bodies
R3906 T14187 T14188 amod mature,SACs
R3907 T14188 T14186 pobj SACs,of
R3908 T14189 T14190 punct [,37
R3909 T14190 T14180 parataxis 37,marks
R3910 T14191 T14190 punct ],37
R3911 T14192 T14167 punct .,is
R3912 T14194 T14195 nsubjpass Chat,seen
R3913 T14196 T14195 auxpass was,seen
R3914 T14197 T14195 prep in,seen
R3915 T14198 T14199 amod fewer,cells
R3916 T14199 T14197 pobj cells,in
R3917 T14200 T14199 prep in,cells
R3918 T14201 T14202 det the,retina
R3919 T14202 T14200 pobj retina,in
R3920 T14203 T14202 amod mature,retina
R3921 T14204 T14205 compound Rb,KO
R3922 T14205 T14202 compound KO,retina
R3923 T14206 T14195 punct ", ",seen
R3924 T14207 T14195 cc and,seen
R3925 T14208 T14195 conj was,seen
R3926 T14209 T14208 acomp present,was
R3927 T14210 T14208 prep in,was
R3928 T14211 T14212 det the,soma
R3929 T14212 T14210 pobj soma,in
R3930 T14213 T14208 cc but,was
R3931 T14214 T14208 conj absent,was
R3932 T14215 T14214 prep from,absent
R3933 T14216 T14215 pobj processes,from
R3934 T14217 T14218 punct (,3B
R3935 T14218 T14208 parataxis 3B,was
R3936 T14219 T14218 compound Figure,3B
R3937 T14220 T14218 punct ),3B
R3938 T14221 T14195 punct .,seen
R3939 T14223 T14224 nsubj We,obtained
R3940 T14225 T14226 amod similar,results
R3941 T14226 T14224 dobj results,obtained
R3942 T14227 T14224 prep for,obtained
R3943 T14228 T14227 pobj Sv2c,for
R3944 T14229 T14228 punct ", ",Sv2c
R3945 T14230 T14231 det a,protein
R3946 T14231 T14228 appos protein,Sv2c
R3947 T14232 T14233 amod synaptic,vesicle
R3948 T14233 T14231 compound vesicle,protein
R3949 T14234 T14231 acl found,protein
R3950 T14235 T14234 prep in,found
R3951 T14236 T14235 pobj SACs,in
R3952 T14237 T14238 punct [,40
R3953 T14238 T14234 parataxis 40,found
R3954 T14239 T14238 punct ],40
R3955 T14240 T14228 punct ;,Sv2c
R3956 T14241 T14228 conj Kcnc1b,Sv2c
R3957 T14242 T14241 cc and,Kcnc1b
R3958 T14243 T14241 conj Kcnc2,Kcnc1b
R3959 T14244 T14241 punct ", ",Kcnc1b
R3960 T14245 T14246 compound potassium,channels
R3961 T14246 T14241 appos channels,Kcnc1b
R3962 T14247 T14246 acl expressed,channels
R3963 T14248 T14247 prep on,expressed
R3964 T14249 T14250 compound SAC,soma
R3965 T14250 T14248 pobj soma,on
R3966 T14251 T14250 cc and,soma
R3967 T14252 T14250 conj dendrites,soma
R3968 T14253 T14254 advmod as,as
R3969 T14254 T14250 cc as,soma
R3970 T14255 T14254 advmod well,as
R3971 T14256 T14257 det a,number
R3972 T14257 T14250 conj number,soma
R3973 T14258 T14259 advmod very,small
R3974 T14259 T14257 amod small,number
R3975 T14260 T14257 prep of,number
R3976 T14261 T14262 compound ganglion,cells
R3977 T14262 T14260 pobj cells,of
R3978 T14263 T14264 punct [,41
R3979 T14264 T14247 parataxis 41,expressed
R3980 T14265 T14264 punct ],41
R3981 T14266 T14241 punct ;,Kcnc1b
R3982 T14267 T14268 nmod gamma,acid
R3983 T14268 T14241 conj acid,Kcnc1b
R3984 T14269 T14268 punct -,acid
R3985 T14270 T14268 amod aminobutyric,acid
R3986 T14271 T14268 punct (,acid
R3987 T14272 T14268 appos GABA,acid
R3988 T14273 T14268 punct ),acid
R3989 T14274 T14268 punct ", ",acid
R3990 T14275 T14276 det an,neurotransmitter
R3991 T14276 T14268 appos neurotransmitter,acid
R3992 T14277 T14276 amod inhibitory,neurotransmitter
R3993 T14278 T14276 relcl present,neurotransmitter
R3994 T14279 T14278 prep in,present
R3995 T14280 T14281 quantmod about,half
R3996 T14281 T14279 pobj half,in
R3997 T14282 T14281 prep of,half
R3998 T14283 T14284 amod amacrine,cells
R3999 T14284 T14282 pobj cells,of
R4000 T14285 T14281 prep including,half
R4001 T14286 T14285 pobj SACs,including
R4002 T14287 T14281 punct ", ",half
R4003 T14288 T14289 advmod as,as
R4004 T14289 T14281 cc as,half
R4005 T14290 T14289 advmod well,as
R4006 T14291 T14281 conj horizontal,half
R4007 T14292 T14291 cc and,horizontal
R4008 T14293 T14294 det some,bipolar
R4009 T14294 T14295 nmod bipolar,neurons
R4010 T14295 T14291 conj neurons,horizontal
R4011 T14296 T14297 punct [,37
R4012 T14297 T14278 parataxis 37,present
R4013 T14298 T14297 punct ],37
R4014 T14299 T14268 punct ;,acid
R4015 T14300 T14268 cc and,acid
R4016 T14301 T14268 conj Calb1,acid
R4017 T14302 T14301 punct (,Calb1
R4018 T14303 T14301 appos calbindin,Calb1
R4019 T14304 T14301 punct ),Calb1
R4020 T14305 T14301 punct ", ",Calb1
R4021 T14306 T14307 dep which,expressed
R4022 T14307 T14301 relcl expressed,Calb1
R4023 T14308 T14307 auxpass is,expressed
R4024 T14309 T14307 prep in,expressed
R4025 T14310 T14311 amod many,cells
R4026 T14311 T14309 pobj cells,in
R4027 T14312 T14311 amod amacrine,cells
R4028 T14313 T14307 cc and,expressed
R4029 T14314 T14307 conj labels,expressed
R4030 T14315 T14316 compound SAC,process
R4031 T14316 T14314 dobj process,labels
R4032 T14317 T14314 advmod faintly,labels
R4033 T14318 T14319 punct (,S7A
R4034 T14319 T14314 parataxis S7A,labels
R4035 T14320 T14319 compound Figure,S7A
R4036 T14321 T14319 cc and,S7A
R4037 T14322 T14319 conj S7B,S7A
R4038 T14323 T14319 punct ;,S7A
R4039 T14324 T14325 compound Table,S1
R4040 T14325 T14319 conj S1,S7A
R4041 T14326 T14325 punct ;,S1
R4042 T14327 T14325 cc and,S1
R4043 T14328 T14329 nsubj data,shown
R4044 T14329 T14325 conj shown,S1
R4045 T14330 T14329 neg not,shown
R4046 T14331 T14329 punct ),shown
R4047 T14332 T14224 punct .,obtained
R4048 T14334 T14335 advmod Finally,examined
R4049 T14336 T14335 punct ", ",examined
R4050 T14337 T14335 nsubj we,examined
R4051 T14338 T14335 advmod also,examined
R4052 T14339 T14340 det the,effect
R4053 T14340 T14335 dobj effect,examined
R4054 T14341 T14340 prep of,effect
R4055 T14342 T14343 compound Rb,deletion
R4056 T14343 T14341 pobj deletion,of
R4057 T14344 T14340 prep on,effect
R4058 T14345 T14346 compound SAC,differentiation
R4059 T14346 T14344 pobj differentiation,on
R4060 T14347 T14335 advcl using,examined
R4061 T14348 T14349 det a,transgene
R4062 T14349 T14347 dobj transgene,using
R4063 T14350 T14351 compound Chx10,Cre
R4064 T14351 T14349 compound Cre,transgene
R4065 T14352 T14351 punct -,Cre
R4066 T14353 T14354 dep that,expressed
R4067 T14354 T14349 relcl expressed,transgene
R4068 T14355 T14354 auxpass is,expressed
R4069 T14356 T14354 prep in,expressed
R4070 T14357 T14358 det a,pattern
R4071 T14358 T14356 pobj pattern,in
R4072 T14359 T14358 amod mosaic,pattern
R4073 T14360 T14354 prep across,expressed
R4074 T14361 T14362 det the,retina
R4075 T14362 T14360 pobj retina,across
R4076 T14363 T14354 punct ", ",expressed
R4077 T14364 T14354 advcl generating,expressed
R4078 T14365 T14364 dobj patches,generating
R4079 T14366 T14365 prep of,patches
R4080 T14367 T14368 npadvmod Cre,expressing
R4081 T14368 T14370 amod expressing,cells
R4082 T14369 T14368 punct -,expressing
R4083 T14370 T14366 pobj cells,of
R4084 T14371 T14372 punct [,42
R4085 T14372 T14364 parataxis 42,generating
R4086 T14373 T14372 punct ],42
R4087 T14374 T14335 punct .,examined
R4088 T14376 T14377 advcl Consistent,observed
R4089 T14378 T14376 prep with,Consistent
R4090 T14379 T14380 det the,pattern
R4091 T14380 T14378 pobj pattern,with
R4092 T14381 T14382 amod mosaic,deletion
R4093 T14382 T14380 compound deletion,pattern
R4094 T14383 T14377 punct ", ",observed
R4095 T14384 T14377 nsubj we,observed
R4096 T14385 T14386 advmod markedly,reduced
R4097 T14386 T14387 amod reduced,staining
R4098 T14387 T14377 dobj staining,observed
R4099 T14388 T14389 compound Chat,Slc18a3
R4100 T14389 T14387 compound Slc18a3,staining
R4101 T14390 T14389 punct /,Slc18a3
R4102 T14391 T14377 prep in,observed
R4103 T14392 T14393 det the,IPL
R4104 T14393 T14391 pobj IPL,in
R4105 T14394 T14393 prep of,IPL
R4106 T14395 T14396 nmod Chx10,Cre
R4107 T14396 T14398 nmod Cre,retina
R4108 T14397 T14396 punct -,Cre
R4109 T14398 T14394 pobj retina,of
R4110 T14399 T14396 punct ;,Cre
R4111 T14400 T14401 compound RbloxP,loxP
R4112 T14401 T14396 appos loxP,Cre
R4113 T14402 T14401 punct /,loxP
R4114 T14403 T14377 prep compared,observed
R4115 T14404 T14403 prep to,compared
R4116 T14405 T14404 pobj WT,to
R4117 T14406 T14407 punct (,S7C
R4118 T14407 T14377 parataxis S7C,observed
R4119 T14408 T14407 compound Figure,S7C
R4120 T14409 T14407 punct ),S7C
R4121 T14410 T14377 punct .,observed
R4122 T14412 T14413 advmod Together,suggest
R4123 T14414 T14413 punct ", ",suggest
R4124 T14415 T14416 det these,results
R4125 T14416 T14413 nsubj results,suggest
R4126 T14417 T14418 det a,role
R4127 T14418 T14413 dobj role,suggest
R4128 T14419 T14418 prep for,role
R4129 T14420 T14419 pobj Rb,for
R4130 T14421 T14418 prep in,role
R4131 T14422 T14423 compound SAC,differentiation
R4132 T14423 T14421 pobj differentiation,in
R4133 T14424 T14413 punct .,suggest
R4134 T14426 T14427 det The,findings
R4135 T14427 T14429 nsubj findings,indicate
R4136 T14428 T14427 amod above,findings
R4137 T14430 T14429 aux could,indicate
R4138 T14431 T14432 det a,defect
R4139 T14432 T14429 dobj defect,indicate
R4140 T14433 T14432 prep in,defect
R4141 T14434 T14435 compound SAC,specification
R4142 T14435 T14433 pobj specification,in
R4143 T14436 T14435 punct ", ",specification
R4144 T14437 T14438 compound SAC,survival
R4145 T14438 T14435 conj survival,specification
R4146 T14439 T14438 punct ", ",survival
R4147 T14440 T14438 cc or,survival
R4148 T14441 T14442 det the,levels
R4149 T14442 T14438 conj levels,survival
R4150 T14443 T14442 cc and,levels
R4151 T14444 T14443 punct /,and
R4152 T14445 T14443 cc or,and
R4153 T14446 T14442 conj transport,levels
R4154 T14447 T14442 prep of,levels
R4155 T14448 T14449 det the,markers
R4156 T14449 T14447 pobj markers,of
R4157 T14450 T14449 acl described,markers
R4158 T14451 T14450 advmod above,described
R4159 T14452 T14429 punct .,indicate
R4160 T14454 T14455 nsubj Camk2a,marks
R4161 T14456 T14457 preconj both,SACs
R4162 T14457 T14455 dobj SACs,marks
R4163 T14458 T14457 cc and,SACs
R4164 T14459 T14460 compound ganglion,cells
R4165 T14460 T14457 conj cells,SACs
R4166 T14461 T14462 punct [,37
R4167 T14462 T14455 parataxis 37,marks
R4168 T14463 T14462 punct ],37
R4169 T14464 T14455 punct ", ",marks
R4170 T14465 T14455 cc but,marks
R4171 T14466 T14467 mark because,eliminated
R4172 T14467 T14471 advcl eliminated,is
R4173 T14468 T14469 compound ganglion,cells
R4174 T14469 T14467 nsubjpass cells,eliminated
R4175 T14470 T14467 auxpass are,eliminated
R4176 T14471 T14455 conj is,marks
R4177 T14472 T14467 prep in,eliminated
R4178 T14473 T14474 det the,retina
R4179 T14474 T14472 pobj retina,in
R4180 T14475 T14476 compound Rb,KO
R4181 T14476 T14474 compound KO,retina
R4182 T14477 T14471 punct ", ",is
R4183 T14478 T14471 nsubj Camk2a,is
R4184 T14479 T14480 det a,marker
R4185 T14480 T14471 attr marker,is
R4186 T14481 T14480 amod specific,marker
R4187 T14482 T14480 compound SAC,marker
R4188 T14483 T14471 prep in,is
R4189 T14484 T14485 det this,context
R4190 T14485 T14483 pobj context,in
R4191 T14486 T14471 punct .,is
R4192 T14488 T14489 advmod Importantly,were
R4193 T14490 T14489 punct ", ",were
R4194 T14491 T14489 nsubj Camk2a,were
R4195 T14492 T14491 punct +,Camk2a
R4196 T14493 T14491 appos tracks,Camk2a
R4197 T14494 T14493 cc and,tracks
R4198 T14495 T14493 conj dendrites,tracks
R4199 T14496 T14489 acomp present,were
R4200 T14497 T14489 prep in,were
R4201 T14498 T14499 preconj both,retina
R4202 T14499 T14497 pobj retina,in
R4203 T14500 T14499 det the,retina
R4204 T14501 T14499 nmod WT,retina
R4205 T14502 T14501 cc and,WT
R4206 T14503 T14504 compound Rb,KO
R4207 T14504 T14501 conj KO,WT
R4208 T14505 T14506 punct (,3B
R4209 T14506 T14489 parataxis 3B,were
R4210 T14507 T14506 compound Figure,3B
R4211 T14508 T14506 punct ),3B
R4212 T14509 T14489 punct ", ",were
R4213 T14510 T14489 cc and,were
R4214 T14511 T14512 det the,number
R4215 T14512 T14513 nsubj number,was
R4216 T14513 T14489 conj was,were
R4217 T14514 T14512 prep of,number
R4218 T14515 T14516 nmod Camk2a,soma
R4219 T14516 T14514 pobj soma,of
R4220 T14517 T14515 punct +,Camk2a
R4221 T14518 T14513 acomp similar,was
R4222 T14519 T14513 prep in,was
R4223 T14520 T14521 nmod WT,retina
R4224 T14521 T14519 pobj retina,in
R4225 T14522 T14520 cc and,WT
R4226 T14523 T14524 compound Rb,KO
R4227 T14524 T14520 conj KO,WT
R4228 T14525 T14513 prep at,was
R4229 T14526 T14525 pobj P30,at
R4230 T14527 T14526 cc and,P30
R4231 T14528 T14526 conj beyond,P30
R4232 T14529 T14513 punct ", ",was
R4233 T14530 T14531 mark although,stained
R4234 T14531 T14513 advcl stained,was
R4235 T14532 T14533 amod fewer,cells
R4236 T14533 T14531 nsubj cells,stained
R4237 T14534 T14531 prep in,stained
R4238 T14535 T14536 compound Rb,KO
R4239 T14536 T14537 compound KO,retina
R4240 T14537 T14534 pobj retina,in
R4241 T14538 T14531 prep at,stained
R4242 T14539 T14538 pobj P18,at
R4243 T14540 T14531 punct ", ",stained
R4244 T14541 T14531 advcl suggesting,stained
R4245 T14542 T14543 det a,delay
R4246 T14543 T14541 dobj delay,suggesting
R4247 T14544 T14543 prep in,delay
R4248 T14545 T14546 poss its,appearance
R4249 T14546 T14544 pobj appearance,in
R4250 T14547 T14548 punct (,3C
R4251 T14548 T14541 parataxis 3C,suggesting
R4252 T14549 T14548 compound Figures,3C
R4253 T14550 T14548 cc and,3C
R4254 T14551 T14548 conj S6B,3C
R4255 T14552 T14548 punct ),3C
R4256 T14553 T14513 punct .,was
R4257 T14555 T14556 advmod Thus,required
R4258 T14557 T14556 punct ", ",required
R4259 T14558 T14556 nsubjpass Rb,required
R4260 T14559 T14556 auxpass is,required
R4261 T14560 T14556 neg not,required
R4262 T14561 T14556 prep for,required
R4263 T14562 T14563 compound SAC,survival
R4264 T14563 T14561 pobj survival,for
R4265 T14564 T14561 cc or,for
R4266 T14565 T14561 conj for,for
R4267 T14566 T14567 compound process,outgrowth
R4268 T14567 T14565 pobj outgrowth,for
R4269 T14568 T14556 punct ", ",required
R4270 T14569 T14556 cc but,required
R4271 T14570 T14569 advmod rather,but
R4272 T14571 T14572 nsubj it,seems
R4273 T14572 T14556 conj seems,required
R4274 T14573 T14574 aux to,regulate
R4275 T14574 T14572 xcomp regulate,seems
R4276 T14575 T14576 det the,expression
R4277 T14576 T14574 dobj expression,regulate
R4278 T14577 T14576 cc and,expression
R4279 T14578 T14577 punct /,and
R4280 T14579 T14577 cc or,and
R4281 T14580 T14576 conj stability,expression
R4282 T14581 T14576 prep of,expression
R4283 T14582 T14581 pobj Calb2,of
R4284 T14583 T14582 punct ", ",Calb2
R4285 T14584 T14582 conj Calb1,Calb2
R4286 T14585 T14584 punct ", ",Calb1
R4287 T14586 T14584 conj Chat,Calb1
R4288 T14587 T14586 punct ", ",Chat
R4289 T14588 T14586 conj Slc18a3,Chat
R4290 T14589 T14588 punct ", ",Slc18a3
R4291 T14590 T14588 conj Sv2c,Slc18a3
R4292 T14591 T14590 punct ", ",Sv2c
R4293 T14592 T14590 conj Kcnc1b,Sv2c
R4294 T14593 T14592 punct ", ",Kcnc1b
R4295 T14594 T14592 conj Kcnc2,Kcnc1b
R4296 T14595 T14594 punct ", ",Kcnc2
R4297 T14596 T14594 cc and,Kcnc2
R4298 T14597 T14594 conj GABA,Kcnc2
R4299 T14598 T14574 prep in,regulate
R4300 T14599 T14598 pobj SACs,in
R4301 T14600 T14572 punct ", ",seems
R4302 T14601 T14572 cc but,seems
R4303 T14602 T14572 conj leaves,seems
R4304 T14603 T14604 compound Camk2a,expression
R4305 T14604 T14602 dobj expression,leaves
R4306 T14605 T14606 advmod virtually,unaffected
R4307 T14606 T14602 oprd unaffected,leaves
R4308 T14607 T14572 punct .,seems
R4309 T14609 T14610 det The,presence
R4310 T14610 T14611 nsubj presence,suggests
R4311 T14612 T14610 prep of,presence
R4312 T14613 T14612 pobj Chat,of
R4313 T14614 T14610 prep in,presence
R4314 T14615 T14616 det some,bodies
R4315 T14616 T14614 pobj bodies,in
R4316 T14617 T14616 compound cell,bodies
R4317 T14618 T14614 cc but,in
R4318 T14619 T14620 neg never,in
R4319 T14620 T14614 conj in,in
R4320 T14621 T14620 pobj processes,in
R4321 T14622 T14623 punct (,3B
R4322 T14623 T14610 parataxis 3B,presence
R4323 T14624 T14623 compound Figure,3B
R4324 T14625 T14623 punct ),3B
R4325 T14626 T14611 advmod also,suggests
R4326 T14627 T14628 det a,defect
R4327 T14628 T14611 dobj defect,suggests
R4328 T14629 T14628 compound transport,defect
R4329 T14630 T14611 punct .,suggests
R4330 T14632 T14633 det The,pattern
R4331 T14633 T14635 nsubj pattern,supported
R4332 T14634 T14633 amod developmental,pattern
R4333 T14636 T14633 prep of,pattern
R4334 T14637 T14638 compound Slc18a3,expression
R4335 T14638 T14636 pobj expression,of
R4336 T14639 T14635 advmod also,supported
R4337 T14640 T14641 det this,notion
R4338 T14641 T14635 dobj notion,supported
R4339 T14642 T14635 punct .,supported
R4340 T14644 T14645 prep In,is
R4341 T14646 T14647 amod mature,SACs
R4342 T14647 T14644 pobj SACs,In
R4343 T14648 T14647 compound WT,SACs
R4344 T14649 T14645 nsubj Slc18a3,is
R4345 T14650 T14651 advmod only,in
R4346 T14651 T14645 prep in,is
R4347 T14652 T14651 pobj processes,in
R4348 T14653 T14645 punct ", ",is
R4349 T14654 T14645 cc but,is
R4350 T14655 T14656 prep in,found
R4351 T14656 T14645 conj found,is
R4352 T14657 T14658 amod early,SACs
R4353 T14658 T14655 pobj SACs,in
R4354 T14659 T14658 amod postnatal,SACs
R4355 T14660 T14656 punct ", ",found
R4356 T14661 T14656 nsubjpass it,found
R4357 T14662 T14656 auxpass is,found
R4358 T14663 T14656 prep in,found
R4359 T14664 T14665 det the,body
R4360 T14665 T14663 pobj body,in
R4361 T14666 T14665 compound cell,body
R4362 T14667 T14656 punct ", ",found
R4363 T14668 T14656 cc and,found
R4364 T14669 T14656 conj moves,found
R4365 T14670 T14669 prep into,moves
R4366 T14671 T14672 amod emerging,processes
R4367 T14672 T14670 pobj processes,into
R4368 T14673 T14669 prep at,moves
R4369 T14674 T14675 advmod approximately,P4
R4370 T14675 T14673 pobj P4,at
R4371 T14676 T14677 punct –,P6
R4372 T14677 T14675 prep P6,P4
R4373 T14678 T14656 punct .,found
R4374 T14680 T14681 mark As,noted
R4375 T14699 T14686 punct ;,was
R4376 T14681 T14682 advcl noted,was
R4377 T14700 T14686 prep at,was
R4378 T14682 T14686 ccomp was,was
R4379 T14701 T14700 pobj P4,at
R4380 T14683 T14681 advmod above,noted
R4381 T14702 T14701 cc or,P4
R4382 T14703 T14701 conj P5,P4
R4383 T14684 T14682 punct ", ",was
R4384 T14704 T14686 nsubj it,was
R4385 T14705 T14686 prep in,was
R4386 T14685 T14682 nsubj Slc18a3,was
R4387 T14706 T14707 compound cell,bodies
R4388 T14707 T14705 pobj bodies,in
R4389 T14708 T14686 punct ", ",was
R4390 T14687 T14682 acomp absent,was
R4391 T14709 T14686 cc yet,was
R4392 T14710 T14686 conj was,was
R4393 T14688 T14682 prep at,was
R4394 T14711 T14710 advmod rarely,was
R4395 T14712 T14710 acomp present,was
R4396 T14689 T14688 pobj P18,at
R4397 T14713 T14710 prep in,was
R4398 T14714 T14715 compound Rb,KO
R4399 T14690 T14682 prep in,was
R4400 T14715 T14716 compound KO,processes
R4401 T14716 T14713 pobj processes,in
R4402 T14717 T14718 punct (,4A
R4403 T14718 T14710 parataxis 4A,was
R4404 T14691 T14692 det the,retina
R4405 T14719 T14718 compound Figures,4A
R4406 T14720 T14718 cc and,4A
R4407 T14692 T14690 pobj retina,in
R4408 T14721 T14718 conj S6,4A
R4409 T14722 T14718 punct ),4A
R4410 T14723 T14686 punct .,was
R4411 T14693 T14694 compound Rb,KO
R4412 T14694 T14692 compound KO,retina
R4413 T14725 T14726 nsubj Slc18a3,became
R4414 T14727 T14728 advmod virtually,undetectable
R4415 T14728 T14726 acomp undetectable,became
R4416 T14695 T14696 punct (,3A
R4417 T14729 T14726 prep in,became
R4418 T14730 T14731 compound Rb,KO
R4419 T14696 T14682 parataxis 3A,was
R4420 T14731 T14732 compound KO,SACs
R4421 T14732 T14729 pobj SACs,in
R4422 T14733 T14726 prep by,became
R4423 T14734 T14733 pobj P8,by
R4424 T14697 T14696 compound Figure,3A
R4425 T14735 T14736 punct (,3C
R4426 T14736 T14726 parataxis 3C,became
R4427 T14737 T14736 compound Figures,3C
R4428 T14738 T14736 cc and,3C
R4429 T14739 T14736 conj S6C,3C
R4430 T14740 T14736 punct ),3C
R4431 T14741 T14726 punct .,became
R4432 T14698 T14696 punct ),3A
R4433 T14743 T14744 det These,data
R4434 T14744 T14745 nsubj data,suggest
R4435 T14746 T14747 mark that,affects
R4436 T14747 T14745 ccomp affects,suggest
R4437 T14748 T14747 nsubj Rb,affects
R4438 T14749 T14750 preconj both,stability
R4439 T14750 T14747 dobj stability,affects
R4440 T14751 T14750 det the,stability
R4441 T14752 T14750 compound synthesis,stability
R4442 T14753 T14750 punct /,stability
R4443 T14754 T14750 cc and,stability
R4444 T14755 T14750 conj transport,stability
R4445 T14756 T14750 prep of,stability
R4446 T14757 T14758 compound SAC,markers
R4447 T14758 T14756 pobj markers,of
R4448 T14759 T14745 punct .,suggest
R4453 T16394 T16395 nsubj Rb,Regulates
R4454 T16396 T16397 compound SAC,Differentiation
R4455 T16397 T16395 dobj Differentiation,Regulates
R4456 T16398 T16395 prep through,Regulates
R4457 T16399 T16398 pobj E2f3,through
R4458 T16401 T16402 nsubj Rb,binds
R4459 T16403 T16404 amod more,100
R4460 T16404 T16406 nummod 100,proteins
R4461 T16405 T16404 quantmod than,100
R4462 T16406 T16402 dobj proteins,binds
R4463 T16407 T16408 punct [,43
R4464 T16408 T16402 parataxis 43,binds
R4465 T16409 T16408 punct ],43
R4466 T16410 T16402 cc and,binds
R4467 T16411 T16412 prep in,thought
R4468 T16412 T16402 conj thought,binds
R4469 T16413 T16414 det some,cells
R4470 T16414 T16411 pobj cells,in
R4471 T16415 T16414 amod non-neuronal,cells
R4472 T16416 T16414 punct ", ",cells
R4473 T16417 T16418 amod such,as
R4474 T16418 T16414 prep as,cells
R4475 T16419 T16420 amod skeletal,muscle
R4476 T16420 T16418 pobj muscle,as
R4477 T16421 T16420 punct ", ",muscle
R4478 T16422 T16420 conj adipocytes,muscle
R4479 T16423 T16422 punct ", ",adipocytes
R4480 T16424 T16422 cc and,adipocytes
R4481 T16425 T16422 conj bone,adipocytes
R4482 T16426 T16412 punct ", ",thought
R4483 T16427 T16412 nsubjpass Rb,thought
R4484 T16428 T16412 auxpass is,thought
R4485 T16429 T16430 aux to,bind
R4486 T16430 T16412 xcomp bind,thought
R4487 T16431 T16430 cc and,bind
R4488 T16432 T16430 conj potentiate,bind
R4489 T16433 T16434 npadvmod tissue,specific
R4490 T16434 T16436 amod specific,factors
R4491 T16435 T16434 punct -,specific
R4492 T16436 T16432 dobj factors,potentiate
R4493 T16437 T16436 compound transcription,factors
R4494 T16438 T16439 dep that,promote
R4495 T16439 T16436 relcl promote,factors
R4496 T16440 T16439 dobj differentiation,promote
R4497 T16441 T16442 punct [,31
R4498 T16442 T16432 parataxis 31,potentiate
R4499 T16443 T16444 punct –,33
R4500 T16444 T16442 prep 33,31
R4501 T16445 T16442 punct ],31
R4502 T16446 T16412 punct .,thought
R4503 T16448 T16449 advmod Thus,expected
R4504 T16450 T16449 punct ", ",expected
R4505 T16451 T16449 nsubj we,expected
R4506 T16452 T16453 mark that,interact
R4507 T16453 T16449 ccomp interact,expected
R4508 T16454 T16453 nsubj Rb,interact
R4509 T16455 T16453 aux might,interact
R4510 T16456 T16453 prep with,interact
R4511 T16457 T16458 npadvmod retina,specific
R4512 T16458 T16460 amod specific,factors
R4513 T16459 T16458 punct -,specific
R4514 T16460 T16456 pobj factors,with
R4515 T16461 T16462 aux to,facilitate
R4516 T16462 T16453 advcl facilitate,interact
R4517 T16463 T16464 compound SAC,differentiation
R4518 T16464 T16462 dobj differentiation,facilitate
R4519 T16465 T16449 punct .,expected
R4520 T16467 T16468 det A,role
R4521 T16468 T16470 nsubjpass role,described
R4522 T16469 T16468 amod direct,role
R4523 T16471 T16468 prep for,role
R4524 T16472 T16471 pobj E2f,for
R4525 T16473 T16468 prep in,role
R4526 T16474 T16473 pcomp mediating,in
R4527 T16475 T16476 npadvmod Rb,dependent
R4528 T16476 T16478 amod dependent,defects
R4529 T16477 T16476 punct -,dependent
R4530 T16478 T16474 dobj defects,mediating
R4531 T16479 T16478 compound differentiation,defects
R4532 T16480 T16478 punct (,defects
R4533 T16481 T16478 amod independent,defects
R4534 T16482 T16481 prep of,independent
R4535 T16483 T16484 nmod cell,cycle
R4536 T16484 T16485 nmod cycle,defects
R4537 T16485 T16482 pobj defects,of
R4538 T16486 T16484 cc or,cycle
R4539 T16487 T16484 conj death,cycle
R4540 T16488 T16470 punct ),described
R4541 T16489 T16470 aux has,described
R4542 T16490 T16470 prep to,described
R4543 T16491 T16492 poss our,knowledge
R4544 T16492 T16490 pobj knowledge,to
R4545 T16493 T16470 neg not,described
R4546 T16494 T16470 auxpass been,described
R4547 T16495 T16470 punct ", ",described
R4548 T16496 T16470 cc but,described
R4549 T16497 T16498 mark because,regulate
R4550 T16498 T16501 advcl regulate,tested
R4551 T16499 T16498 nsubj E2f,regulate
R4552 T16500 T16498 aux can,regulate
R4553 T16501 T16470 conj tested,described
R4554 T16502 T16503 det some,genes
R4555 T16503 T16498 dobj genes,regulate
R4556 T16504 T16503 compound differentiation,genes
R4557 T16505 T16506 punct [,44
R4558 T16506 T16498 parataxis 44,regulate
R4559 T16507 T16508 punct –,48
R4560 T16508 T16506 prep 48,44
R4561 T16509 T16506 punct ],44
R4562 T16510 T16501 punct ", ",tested
R4563 T16511 T16501 nsubj we,tested
R4564 T16512 T16501 advmod first,tested
R4565 T16513 T16514 mark whether,perturb
R4566 T16514 T16501 ccomp perturb,tested
R4567 T16515 T16514 nsubj E2f2,perturb
R4568 T16516 T16515 cc or,E2f2
R4569 T16517 T16515 conj E2f3,E2f2
R4570 T16518 T16514 aux might,perturb
R4571 T16519 T16520 compound Rb,KO
R4572 T16520 T16521 compound KO,SAC
R4573 T16521 T16522 compound SAC,maturation
R4574 T16522 T16514 dobj maturation,perturb
R4575 T16523 T16501 punct .,tested
R4576 T16525 T16526 prep At,suppressed
R4577 T16527 T16528 amod multiple,points
R4578 T16528 T16525 pobj points,At
R4579 T16529 T16528 compound time,points
R4580 T16530 T16526 punct ", ",suppressed
R4581 T16531 T16532 compound E2f1,deletion
R4582 T16532 T16526 nsubj deletion,suppressed
R4583 T16533 T16534 amod ectopic,mitosis
R4584 T16534 T16526 dobj mitosis,suppressed
R4585 T16535 T16536 punct (,cells
R4586 T16536 T16526 parataxis cells,suppressed
R4587 T16537 T16536 nmod PH3,cells
R4588 T16538 T16537 punct +,PH3
R4589 T16539 T16536 punct ),cells
R4590 T16540 T16526 punct ", ",suppressed
R4591 T16541 T16526 cc but,suppressed
R4592 T16542 T16543 aux did,reverse
R4593 T16543 T16526 conj reverse,suppressed
R4594 T16544 T16543 neg not,reverse
R4595 T16545 T16546 det the,defect
R4596 T16546 T16543 dobj defect,reverse
R4597 T16547 T16546 compound SAC,defect
R4598 T16548 T16526 punct ", ",suppressed
R4599 T16549 T16526 cc and,suppressed
R4600 T16550 T16551 compound E2f2,deletion
R4601 T16551 T16552 nsubj deletion,had
R4602 T16552 T16526 conj had,suppressed
R4603 T16553 T16554 det no,effect
R4604 T16554 T16552 dobj effect,had
R4605 T16555 T16554 prep on,effect
R4606 T16556 T16557 preconj either,defect
R4607 T16557 T16555 pobj defect,on
R4608 T16558 T16559 punct (,4A
R4609 T16559 T16552 parataxis 4A,had
R4610 T16560 T16559 compound Figure,4A
R4611 T16561 T16559 punct ),4A
R4612 T16562 T16552 punct .,had
R4613 T16564 T16565 advmod Remarkably,rescued
R4614 T16566 T16565 punct ", ",rescued
R4615 T16567 T16568 mark although,reverse
R4616 T16568 T16565 advcl reverse,rescued
R4617 T16569 T16570 compound E2f3,deletion
R4618 T16570 T16568 nsubj deletion,reverse
R4619 T16571 T16568 aux did,reverse
R4620 T16572 T16568 neg not,reverse
R4621 T16573 T16574 amod ectopic,mitosis
R4622 T16574 T16568 dobj mitosis,reverse
R4623 T16575 T16565 punct ", ",rescued
R4624 T16576 T16565 nsubj it,rescued
R4625 T16577 T16578 nmod Calb2,staining
R4626 T16578 T16565 dobj staining,rescued
R4627 T16579 T16577 punct ", ",Calb2
R4628 T16580 T16577 conj Slc18a3,Calb2
R4629 T16581 T16580 punct ", ",Slc18a3
R4630 T16582 T16580 conj Chat,Slc18a3
R4631 T16583 T16582 punct ", ",Chat
R4632 T16584 T16582 conj GABA,Chat
R4633 T16585 T16584 punct ", ",GABA
R4634 T16586 T16584 conj Kcnc1b,GABA
R4635 T16587 T16586 punct ", ",Kcnc1b
R4636 T16588 T16586 conj Kcnc2,Kcnc1b
R4637 T16589 T16588 punct ", ",Kcnc2
R4638 T16590 T16588 cc and,Kcnc2
R4639 T16591 T16588 conj Sv2c,Kcnc2
R4640 T16592 T16565 prep at,rescued
R4641 T16593 T16594 amod multiple,times
R4642 T16594 T16592 pobj times,at
R4643 T16595 T16596 punct (,4A
R4644 T16596 T16565 parataxis 4A,rescued
R4645 T16597 T16596 compound Figure,4A
R4646 T16598 T16596 cc and,4A
R4647 T16599 T16600 nsubj data,shown
R4648 T16600 T16596 conj shown,4A
R4649 T16601 T16600 neg not,shown
R4650 T16602 T16600 punct ),shown
R4651 T16603 T16565 punct .,rescued
R4652 T16605 T16606 compound Rb,E2f3
R4653 T16606 T16608 compound E2f3,DKO
R4654 T16607 T16606 punct /,E2f3
R4655 T16608 T16609 compound DKO,SAC
R4656 T16609 T16610 compound SAC,tracks
R4657 T16610 T16611 nsubj tracks,were
R4658 T16612 T16613 advmod slightly,more
R4659 T16613 T16614 advmod more,disordered
R4660 T16614 T16611 acomp disordered,were
R4661 T16615 T16614 prep than,disordered
R4662 T16616 T16615 prep in,than
R4663 T16617 T16618 compound WT,retina
R4664 T16618 T16616 pobj retina,in
R4665 T16619 T16611 punct ", ",were
R4666 T16620 T16621 advmod most,likely
R4667 T16621 T16622 advmod likely,because
R4668 T16622 T16611 prep because,were
R4669 T16623 T16622 pcomp of,because
R4670 T16624 T16625 det the,absence
R4671 T16625 T16622 pobj absence,because
R4672 T16626 T16625 prep of,absence
R4673 T16627 T16628 amod synaptic,cells
R4674 T16628 T16626 pobj cells,of
R4675 T16629 T16628 compound partner,cells
R4676 T16630 T16628 punct ", ",cells
R4677 T16631 T16632 dep which,killed
R4678 T16632 T16628 relcl killed,cells
R4679 T16633 T16632 auxpass are,killed
R4680 T16634 T16632 agent by,killed
R4681 T16635 T16634 pobj E2f1,by
R4682 T16636 T16611 punct .,were
R4683 T16638 T16639 advmod Indeed,rescued
R4684 T16640 T16639 punct ", ",rescued
R4685 T16641 T16642 det this,defect
R4686 T16642 T16639 nsubjpass defect,rescued
R4687 T16643 T16642 amod minor,defect
R4688 T16644 T16639 auxpass was,rescued
R4689 T16645 T16639 prep in,rescued
R4690 T16646 T16647 det the,retina
R4691 T16647 T16645 pobj retina,in
R4692 T16648 T16649 nmod Rb,E2f3
R4693 T16649 T16653 nmod E2f3,knockout
R4694 T16650 T16649 punct /,E2f3
R4695 T16651 T16649 nmod E2f1,E2f3
R4696 T16652 T16649 punct /,E2f3
R4697 T16653 T16647 compound knockout,retina
R4698 T16654 T16653 amod triple,knockout
R4699 T16655 T16647 punct ", ",retina
R4700 T16656 T16657 advmod where,rescued
R4701 T16657 T16647 relcl rescued,retina
R4702 T16658 T16659 amod bipolar,cell
R4703 T16659 T16662 compound cell,death
R4704 T16660 T16658 cc and,bipolar
R4705 T16661 T16658 conj ganglion,bipolar
R4706 T16662 T16657 nsubjpass death,rescued
R4707 T16663 T16657 auxpass was,rescued
R4708 T16664 T16657 cc and,rescued
R4709 T16665 T16666 compound SAC,differentiation
R4710 T16666 T16667 nsubjpass differentiation,restored
R4711 T16667 T16657 conj restored,rescued
R4712 T16668 T16667 auxpass was,restored
R4713 T16669 T16670 punct (,4A
R4714 T16670 T16639 parataxis 4A,rescued
R4715 T16671 T16670 compound Figure,4A
R4716 T16672 T16670 punct ),4A
R4717 T16673 T16639 punct .,rescued
R4718 T16675 T16676 compound E2f3,deletion
R4719 T16676 T16677 nsubj deletion,affect
R4720 T16678 T16676 advmod alone,deletion
R4721 T16679 T16677 aux did,affect
R4722 T16680 T16677 neg not,affect
R4723 T16681 T16682 compound SAC,differentiation
R4724 T16682 T16677 dobj differentiation,affect
R4725 T16683 T16684 punct (,4A
R4726 T16684 T16677 parataxis 4A,affect
R4727 T16685 T16684 compound Figure,4A
R4728 T16686 T16684 punct ),4A
R4729 T16687 T16677 punct ;,affect
R4730 T16688 T16689 advmod thus,is
R4731 T16689 T16677 conj is,affect
R4732 T16690 T16689 punct ", ",is
R4733 T16691 T16689 nsubj it,is
R4734 T16692 T16693 amod unleashed,activity
R4735 T16693 T16689 attr activity,is
R4736 T16694 T16693 compound E2f3,activity
R4737 T16695 T16696 dep that,is
R4738 T16696 T16689 ccomp is,is
R4739 T16697 T16696 acomp detrimental,is
R4740 T16698 T16689 punct ", ",is
R4741 T16699 T16689 cc and,is
R4742 T16700 T16701 det the,role
R4743 T16701 T16703 nsubj role,is
R4744 T16702 T16701 amod critical,role
R4745 T16703 T16689 conj is,is
R4746 T16704 T16701 prep for,role
R4747 T16705 T16704 pobj Rb,for
R4748 T16706 T16707 aux to,inhibit
R4749 T16707 T16703 xcomp inhibit,is
R4750 T16708 T16707 dobj E2f3,inhibit
R4751 T16709 T16703 punct .,is
R4752 T16711 T16712 nsubj We,quantified
R4753 T16713 T16714 det the,fraction
R4754 T16714 T16712 dobj fraction,quantified
R4755 T16715 T16714 prep of,fraction
R4756 T16716 T16717 nmod Camk2a,SACs
R4757 T16717 T16715 pobj SACs,of
R4758 T16718 T16716 punct +,Camk2a
R4759 T16719 T16714 prep in,fraction
R4760 T16720 T16721 amod different,genotypes
R4761 T16721 T16719 pobj genotypes,in
R4762 T16722 T16712 cc and,quantified
R4763 T16723 T16712 conj found,quantified
R4764 T16724 T16725 mark that,expressed
R4765 T16725 T16723 ccomp expressed,found
R4766 T16726 T16727 nummod 60,%
R4767 T16727 T16725 nsubj %,expressed
R4768 T16728 T16727 prep of,%
R4769 T16729 T16730 nmod WT,cells
R4770 T16730 T16728 pobj cells,of
R4771 T16731 T16730 nmod P30,cells
R4772 T16732 T16730 nmod Camk2a,cells
R4773 T16733 T16732 punct +,Camk2a
R4774 T16734 T16725 dobj Chat,expressed
R4775 T16735 T16734 cc and,Chat
R4776 T16736 T16734 conj Slc18a3,Chat
R4777 T16737 T16725 punct ", ",expressed
R4778 T16738 T16739 dep which,dropped
R4779 T16739 T16725 ccomp dropped,expressed
R4780 T16740 T16739 prep to,dropped
R4781 T16741 T16742 advmod only,5.6
R4782 T16742 T16743 nummod 5.6,%
R4783 T16743 T16740 pobj %,to
R4784 T16744 T16739 prep in,dropped
R4785 T16745 T16746 det the,retina
R4786 T16746 T16744 pobj retina,in
R4787 T16747 T16748 compound Rb,KO
R4788 T16748 T16746 compound KO,retina
R4789 T16749 T16739 punct ", ",dropped
R4790 T16750 T16739 cc and,dropped
R4791 T16751 T16739 conj remained,dropped
R4792 T16752 T16751 acomp low,remained
R4793 T16753 T16751 prep at,remained
R4794 T16754 T16755 nummod 3.7,%
R4795 T16755 T16753 pobj %,at
R4796 T16756 T16751 prep in,remained
R4797 T16757 T16758 det the,retina
R4798 T16758 T16756 pobj retina,in
R4799 T16759 T16760 compound Rb,E2f1
R4800 T16760 T16762 compound E2f1,DKO
R4801 T16761 T16760 punct /,E2f1
R4802 T16762 T16758 compound DKO,retina
R4803 T16763 T16751 punct ", ",remained
R4804 T16764 T16751 cc but,remained
R4805 T16765 T16751 conj rose,remained
R4806 T16766 T16765 prep to,rose
R4807 T16767 T16768 nummod 91,%
R4808 T16768 T16766 pobj %,to
R4809 T16769 T16765 prep in,rose
R4810 T16770 T16771 det the,retina
R4811 T16771 T16769 pobj retina,in
R4812 T16772 T16773 compound Rb,E2f3
R4813 T16773 T16775 compound E2f3,DKO
R4814 T16774 T16773 punct /,E2f3
R4815 T16775 T16771 compound DKO,retina
R4816 T16776 T16777 punct (,4B
R4817 T16777 T16765 parataxis 4B,rose
R4818 T16778 T16777 compound Figure,4B
R4819 T16779 T16777 punct ),4B
R4820 T16780 T16712 punct .,quantified
R4821 T16782 T16783 det The,fraction
R4822 T16783 T16785 nsubj fraction,is
R4823 T16784 T16783 amod latter,fraction
R4824 T16786 T16785 acomp higher,is
R4825 T16787 T16786 prep than,higher
R4826 T16788 T16787 pobj WT,than
R4827 T16789 T16790 mark because,killed
R4828 T16790 T16785 advcl killed,is
R4829 T16791 T16792 compound ganglion,cells
R4830 T16792 T16790 nsubjpass cells,killed
R4831 T16793 T16792 punct ", ",cells
R4832 T16794 T16795 dep which,make
R4833 T16795 T16792 relcl make,cells
R4834 T16796 T16795 advmod normally,make
R4835 T16797 T16795 prt up,make
R4836 T16798 T16799 punct ~,40
R4837 T16799 T16800 nummod 40,%
R4838 T16800 T16795 dobj %,make
R4839 T16801 T16800 prep of,%
R4840 T16802 T16803 nmod Camk2a,cells
R4841 T16803 T16801 pobj cells,of
R4842 T16804 T16802 punct +,Camk2a
R4843 T16805 T16790 punct ", ",killed
R4844 T16806 T16790 auxpass are,killed
R4845 T16807 T16790 agent by,killed
R4846 T16808 T16807 pobj apoptosis,by
R4847 T16809 T16785 punct .,is
R4848 T16811 T16812 aux To,quantify
R4849 T16812 T16813 advcl quantify,exploited
R4850 T16814 T16815 det the,effect
R4851 T16815 T16812 dobj effect,quantify
R4852 T16816 T16815 prep of,effect
R4853 T16817 T16818 amod different,E2fs
R4854 T16818 T16816 pobj E2fs,of
R4855 T16819 T16815 prep on,effect
R4856 T16820 T16821 amod ectopic,division
R4857 T16821 T16819 pobj division,on
R4858 T16822 T16823 advmod specifically,in
R4859 T16823 T16815 prep in,effect
R4860 T16824 T16823 pobj SACs,in
R4861 T16825 T16813 punct ", ",exploited
R4862 T16826 T16813 nsubj we,exploited
R4863 T16827 T16813 dobj Isl1,exploited
R4864 T16828 T16827 punct (,Isl1
R4865 T16829 T16827 appos Islet1,Isl1
R4866 T16830 T16813 punct ),exploited
R4867 T16831 T16813 punct .,exploited
R4868 T16833 T16834 det This,marker
R4869 T16834 T16835 nsubjpass marker,expressed
R4870 T16835 T16837 ccomp expressed,are
R4871 T16836 T16835 auxpass is,expressed
R4872 T16838 T16835 prep in,expressed
R4873 T16839 T16840 preconj both,SACs
R4874 T16840 T16838 pobj SACs,in
R4875 T16841 T16840 cc and,SACs
R4876 T16842 T16843 compound ganglion,cells
R4877 T16843 T16840 conj cells,SACs
R4878 T16844 T16837 punct ", ",are
R4879 T16845 T16837 advmod thus,are
R4880 T16846 T16847 nmod Isl1,cells
R4881 T16847 T16837 nsubj cells,are
R4882 T16848 T16846 punct +,Isl1
R4883 T16849 T16847 prep in,cells
R4884 T16850 T16851 det the,INL
R4885 T16851 T16849 pobj INL,in
R4886 T16852 T16837 advmod exclusively,are
R4887 T16853 T16837 attr SACs,are
R4888 T16854 T16855 punct [,49
R4889 T16855 T16837 parataxis 49,are
R4890 T16856 T16855 punct ],49
R4891 T16857 T16837 punct .,are
R4892 T16859 T16860 nsubj We,found
R4893 T16861 T16862 mark that,were
R4894 T16862 T16860 ccomp were,found
R4895 T16863 T16864 nummod 98.2,%
R4896 T16864 T16862 nsubj %,were
R4897 T16865 T16864 punct ±,%
R4898 T16866 T16867 nummod 1.8,%
R4899 T16867 T16864 appos %,%
R4900 T16868 T16864 prep of,%
R4901 T16869 T16870 nmod Isl1,cells
R4902 T16870 T16868 pobj cells,of
R4903 T16871 T16869 punct +,Isl1
R4904 T16872 T16864 prep in,%
R4905 T16873 T16874 det the,INL
R4906 T16874 T16872 pobj INL,in
R4907 T16875 T16874 amod forming,INL
R4908 T16876 T16874 amod inner,INL
R4909 T16877 T16864 prep at,%
R4910 T16878 T16877 pobj P5,at
R4911 T16879 T16862 advmod also,were
R4912 T16880 T16862 attr Slc18a3,were
R4913 T16881 T16880 punct +,Slc18a3
R4914 T16882 T16860 punct ", ",found
R4915 T16883 T16860 advcl confirming,found
R4916 T16884 T16885 mark that,is
R4917 T16885 T16883 ccomp is,confirming
R4918 T16886 T16885 nsubj Isl1,is
R4919 T16887 T16888 det an,marker
R4920 T16888 T16885 attr marker,is
R4921 T16889 T16888 amod excellent,marker
R4922 T16890 T16888 compound SAC,marker
R4923 T16891 T16892 punct (,4C
R4924 T16892 T16885 parataxis 4C,is
R4925 T16893 T16892 compound Figure,4C
R4926 T16894 T16892 punct ),4C
R4927 T16895 T16860 punct .,found
R4928 T16897 T16898 advmod Moreover,is
R4929 T16899 T16898 punct ", ",is
R4930 T16900 T16898 nsubj Isl1,is
R4931 T16901 T16900 punct ", ",Isl1
R4932 T16902 T16900 prep unlike,Isl1
R4933 T16903 T16902 pobj Slc18a3,unlike
R4934 T16904 T16898 punct ", ",is
R4935 T16905 T16898 acomp nuclear,is
R4936 T16906 T16898 punct ", ",is
R4937 T16907 T16908 dep which,facilitates
R4938 T16908 T16898 advcl facilitates,is
R4939 T16909 T16908 dobj scoring,facilitates
R4940 T16910 T16909 prep of,scoring
R4941 T16911 T16912 nmod Isl1,cells
R4942 T16912 T16910 pobj cells,of
R4943 T16913 T16911 punct +,Isl1
R4944 T16914 T16911 punct /,Isl1
R4945 T16915 T16911 appos Mki67,Isl1
R4946 T16916 T16915 punct +,Mki67
R4947 T16917 T16898 punct .,is
R4948 T16919 T16920 nsubjpass It,expressed
R4949 T16920 T16923 ccomp expressed,study
R4950 T16921 T16920 auxpass is,expressed
R4951 T16922 T16920 advmod also,expressed
R4952 T16924 T16920 advmod earlier,expressed
R4953 T16925 T16924 prep than,earlier
R4954 T16926 T16925 pobj Slc18a3,than
R4955 T16927 T16920 punct ", ",expressed
R4956 T16928 T16920 advcl permitting,expressed
R4957 T16929 T16928 dobj analysis,permitting
R4958 T16930 T16929 prep of,analysis
R4959 T16931 T16930 pobj SACs,of
R4960 T16932 T16933 advmod soon,after
R4961 T16933 T16928 prep after,permitting
R4962 T16934 T16935 poss their,birth
R4963 T16935 T16933 pobj birth,after
R4964 T16936 T16935 prep at,birth
R4965 T16937 T16938 punct ~,E15
R4966 T16938 T16936 pobj E15,at
R4967 T16939 T16923 punct ;,study
R4968 T16940 T16923 advmod thus,study
R4969 T16941 T16923 punct ", ",study
R4970 T16942 T16923 nsubj we,study
R4971 T16943 T16923 aux could,study
R4972 T16944 T16923 dobj retina,study
R4973 T16945 T16923 prep at,study
R4974 T16946 T16945 pobj P0,at
R4975 T16947 T16946 punct ", ",P0
R4976 T16948 T16949 det a,time
R4977 T16949 T16946 appos time,P0
R4978 T16950 T16951 advmod when,is
R4979 T16951 T16949 relcl is,time
R4980 T16952 T16953 amod ectopic,division
R4981 T16953 T16951 nsubj division,is
R4982 T16954 T16951 acomp high,is
R4983 T16955 T16951 prep in,is
R4984 T16956 T16957 det the,retina
R4985 T16957 T16955 pobj retina,in
R4986 T16958 T16957 amod inner,retina
R4987 T16959 T16945 cc and,at
R4988 T16960 T16961 advmod prior,to
R4989 T16961 T16945 conj to,at
R4990 T16962 T16963 npadvmod Rb,independent
R4991 T16963 T16965 amod independent,exit
R4992 T16964 T16963 punct -,independent
R4993 T16965 T16961 pobj exit,to
R4994 T16966 T16967 compound cell,cycle
R4995 T16967 T16965 compound cycle,exit
R4996 T16968 T16965 acl associated,exit
R4997 T16969 T16968 prep with,associated
R4998 T16970 T16971 amod terminal,differentiation
R4999 T16971 T16969 pobj differentiation,with
R5000 T16972 T16973 punct [,2
R5001 T16973 T16923 parataxis 2,study
R5002 T16974 T16973 punct ],2
R5003 T16975 T16923 punct .,study
R5004 T16977 T16978 prep At,dividing
R5005 T16979 T16977 pobj P0,At
R5006 T16980 T16978 punct ", ",dividing
R5007 T16981 T16982 det no,cells
R5008 T16982 T16978 nsubj cells,dividing
R5009 T16983 T16982 nmod WT,cells
R5010 T16984 T16982 nmod Isl1,cells
R5011 T16985 T16984 punct +,Isl1
R5012 T16986 T16982 prep in,cells
R5013 T16987 T16988 det the,layer
R5014 T16988 T16986 pobj layer,in
R5015 T16989 T16988 amod inner,layer
R5016 T16990 T16988 amod neuroblastic,layer
R5017 T16991 T16988 punct (,layer
R5018 T16992 T16988 appos NBL,layer
R5019 T16993 T16988 punct ),layer
R5020 T16994 T16988 punct (,layer
R5021 T16995 T16996 dep which,is
R5022 T16996 T16988 relcl is,layer
R5023 T16997 T16998 det the,INL
R5024 T16998 T16996 attr INL,is
R5025 T16999 T16998 amod future,INL
R5026 T17000 T16978 punct ),dividing
R5027 T17001 T16978 aux were,dividing
R5028 T17002 T16978 punct ", ",dividing
R5029 T17003 T16978 cc but,dividing
R5030 T17004 T17005 quantmod 57,14
R5031 T17005 T17007 nummod 14,cells
R5032 T17006 T17005 punct ±,14
R5033 T17007 T17013 nsubjpass cells,detected
R5034 T17008 T17007 nmod Isl1,cells
R5035 T17009 T17008 punct +,Isl1
R5036 T17010 T17008 punct /,Isl1
R5037 T17011 T17008 appos Mki67,Isl1
R5038 T17012 T17011 punct +,Mki67
R5039 T17013 T16978 conj detected,dividing
R5040 T17014 T17013 auxpass were,detected
R5041 T17015 T17013 prep in,detected
R5042 T17016 T17017 det the,NBL
R5043 T17017 T17015 pobj NBL,in
R5044 T17018 T17019 nmod Rb,KO
R5045 T17019 T17017 nmod KO,NBL
R5046 T17020 T17017 amod inner,NBL
R5047 T17021 T17022 punct (,4D
R5048 T17022 T17013 parataxis 4D,detected
R5049 T17023 T17022 compound Figure,4D
R5050 T17024 T17022 punct ),4D
R5051 T17025 T17013 punct .,detected
R5052 T17027 T17028 advmod Indeed,dividing
R5053 T17029 T17028 punct ", ",dividing
R5054 T17030 T17031 quantmod about,one
R5055 T17031 T17028 nsubj one,dividing
R5056 T17032 T17031 punct -,one
R5057 T17033 T17031 quantmod third,one
R5058 T17034 T17031 prep of,one
R5059 T17035 T17036 det all,cells
R5060 T17036 T17034 pobj cells,of
R5061 T17037 T17036 nmod Isl1,cells
R5062 T17038 T17037 punct +,Isl1
R5063 T17039 T17031 prep in,one
R5064 T17040 T17041 det the,NBL
R5065 T17041 T17039 pobj NBL,in
R5066 T17042 T17041 amod entire,NBL
R5067 T17043 T17041 amod inner,NBL
R5068 T17044 T17028 aux were,dividing
R5069 T17045 T17028 prep in,dividing
R5070 T17046 T17047 det the,retina
R5071 T17047 T17045 pobj retina,in
R5072 T17048 T17049 compound Rb,KO
R5073 T17049 T17047 compound KO,retina
R5074 T17050 T17028 punct ", ",dividing
R5075 T17051 T17028 cc or,dividing
R5076 T17052 T17053 punct ~,50
R5077 T17053 T17054 nummod 50,%
R5078 T17054 T17028 conj %,dividing
R5079 T17055 T17054 prep in,%
R5080 T17056 T17057 det the,periphery
R5081 T17057 T17055 pobj periphery,in
R5082 T17058 T17059 advmod where,expressed
R5083 T17059 T17057 relcl expressed,periphery
R5084 T17060 T17059 nsubjpass Cre,expressed
R5085 T17061 T17059 auxpass is,expressed
R5086 T17062 T17063 punct (,4E
R5087 T17063 T17054 parataxis 4E,%
R5088 T17064 T17063 compound Figure,4E
R5089 T17065 T17063 cc and,4E
R5090 T17066 T17067 nsubj data,shown
R5091 T17067 T17063 conj shown,4E
R5092 T17068 T17067 neg not,shown
R5093 T17069 T17067 punct ),shown
R5094 T17070 T17054 punct .,%
R5095 T17072 T17073 det This,defect
R5096 T17073 T17074 nsubjpass defect,suppressed
R5097 T17075 T17074 auxpass was,suppressed
R5098 T17076 T17074 prep in,suppressed
R5099 T17077 T17078 det the,retina
R5100 T17078 T17076 pobj retina,in
R5101 T17079 T17080 compound Rb,E2f1
R5102 T17080 T17082 compound E2f1,DKO
R5103 T17081 T17080 punct /,E2f1
R5104 T17082 T17078 compound DKO,retina
R5105 T17083 T17078 punct ", ",retina
R5106 T17084 T17085 advmod where,detected
R5107 T17085 T17078 relcl detected,retina
R5108 T17086 T17085 nsubj we,detected
R5109 T17087 T17088 advmod only,1
R5110 T17088 T17091 nummod 1,SAC
R5111 T17089 T17088 quantmod 1,1
R5112 T17090 T17088 punct ±,1
R5113 T17091 T17085 dobj SAC,detected
R5114 T17092 T17091 compound dividing,SAC
R5115 T17093 T17078 punct ", ",retina
R5116 T17094 T17078 cc but,retina
R5117 T17095 T17094 neg not,but
R5118 T17096 T17097 det the,retina
R5119 T17097 T17078 conj retina,retina
R5120 T17098 T17099 compound Rb,E2f3
R5121 T17099 T17101 compound E2f3,DKO
R5122 T17100 T17099 punct /,E2f3
R5123 T17101 T17097 compound DKO,retina
R5124 T17102 T17097 punct ", ",retina
R5125 T17103 T17104 advmod where,were
R5126 T17104 T17097 relcl were,retina
R5127 T17105 T17104 expl there,were
R5128 T17106 T17107 quantmod 53,8
R5129 T17107 T17109 nummod 8,SACs
R5130 T17108 T17107 punct ±,8
R5131 T17109 T17104 attr SACs,were
R5132 T17110 T17109 amod dividing,SACs
R5133 T17111 T17112 punct (,4D
R5134 T17112 T17104 parataxis 4D,were
R5135 T17113 T17112 compound Figure,4D
R5136 T17114 T17112 cc and,4D
R5137 T17115 T17112 conj 4E,4D
R5138 T17116 T17112 punct ),4D
R5139 T17117 T17074 punct .,suppressed
R5140 T17119 T17120 nsubj We,observed
R5141 T17121 T17122 amod similar,effects
R5142 T17122 T17120 dobj effects,observed
R5143 T17123 T17120 prep at,observed
R5144 T17124 T17123 pobj P0,at
R5145 T17125 T17120 prep with,observed
R5146 T17126 T17125 pobj Calb2,with
R5147 T17127 T17126 punct ", ",Calb2
R5148 T17128 T17129 dep which,marks
R5149 T17129 T17126 relcl marks,Calb2
R5150 T17130 T17131 amod newborn,SACs
R5151 T17131 T17129 dobj SACs,marks
R5152 T17132 T17131 cc and,SACs
R5153 T17133 T17134 amod other,cells
R5154 T17134 T17131 conj cells,SACs
R5155 T17135 T17134 amod amacrine,cells
R5156 T17136 T17137 punct (,shown
R5157 T17137 T17120 parataxis shown,observed
R5158 T17138 T17137 nsubj data,shown
R5159 T17139 T17137 neg not,shown
R5160 T17140 T17137 punct ),shown
R5161 T17141 T17120 punct .,observed
R5162 T17143 T17144 advmod Thus,suppresses
R5163 T17145 T17144 punct ", ",suppresses
R5164 T17146 T17144 prep in,suppresses
R5165 T17147 T17148 compound Rb,KO
R5166 T17148 T17149 compound KO,SACs
R5167 T17149 T17146 pobj SACs,in
R5168 T17150 T17144 punct ", ",suppresses
R5169 T17151 T17152 compound E2f1,deletion
R5170 T17152 T17144 nsubj deletion,suppresses
R5171 T17153 T17154 amod ectopic,division
R5172 T17154 T17144 dobj division,suppresses
R5173 T17155 T17154 cc but,division
R5174 T17156 T17155 neg not,but
R5175 T17157 T17158 amod aberrant,differentiation
R5176 T17158 T17154 conj differentiation,division
R5177 T17159 T17144 punct ", ",suppresses
R5178 T17160 T17161 mark whereas,suppresses
R5179 T17161 T17144 advcl suppresses,suppresses
R5180 T17162 T17163 compound E2f3,deletion
R5181 T17163 T17161 nsubj deletion,suppresses
R5182 T17164 T17165 amod aberrant,differentiation
R5183 T17165 T17161 dobj differentiation,suppresses
R5184 T17166 T17165 cc but,differentiation
R5185 T17167 T17166 neg not,but
R5186 T17168 T17169 amod ectopic,division
R5187 T17169 T17165 conj division,differentiation
R5188 T17170 T17144 punct .,suppresses
R5195 T18365 T18366 amod Specific,Expression
R5196 T18367 T18366 prep of,Expression
R5197 T18368 T18367 pobj E2f3,of
R5198 T18369 T18366 prep in,Expression
R5199 T18370 T18369 pobj SACs,in
R5200 T18371 T18370 cc and,SACs
R5201 T18372 T18373 amod Other,Subsets
R5202 T18373 T18370 conj Subsets,SACs
R5203 T18374 T18373 prep of,Subsets
R5204 T18375 T18376 compound CNS,Neurons
R5205 T18376 T18374 pobj Neurons,of
R5206 T18378 T18379 det The,effect
R5207 T18379 T18381 nsubj effect,be
R5208 T18380 T18379 amod unique,effect
R5209 T18382 T18379 prep of,effect
R5210 T18383 T18382 pobj E2f3,of
R5211 T18384 T18379 prep in,effect
R5212 T18385 T18384 pcomp disrupting,in
R5213 T18386 T18387 det the,differentiation
R5214 T18387 T18385 dobj differentiation,disrupting
R5215 T18388 T18387 prep of,differentiation
R5216 T18389 T18388 pobj SACs,of
R5217 T18390 T18389 cc but,SACs
R5218 T18391 T18390 neg not,but
R5219 T18392 T18393 amod other,neurons
R5220 T18393 T18389 conj neurons,SACs
R5221 T18394 T18393 amod retinal,neurons
R5222 T18395 T18381 aux might,be
R5223 T18396 T18381 prep due,be
R5224 T18397 T18396 pcomp to,due
R5225 T18398 T18399 compound cell,type
R5226 T18399 T18401 npadvmod type,specific
R5227 T18400 T18399 punct -,type
R5228 T18401 T18403 amod specific,expression
R5229 T18402 T18401 punct -,specific
R5230 T18403 T18396 pobj expression,due
R5231 T18404 T18403 cc or,expression
R5232 T18405 T18406 compound cell,type
R5233 T18406 T18408 npadvmod type,specific
R5234 T18407 T18406 punct -,type
R5235 T18408 T18410 amod specific,activity
R5236 T18409 T18408 punct -,specific
R5237 T18410 T18403 conj activity,expression
R5238 T18411 T18403 prep of,expression
R5239 T18412 T18411 pobj E2f3,of
R5240 T18413 T18381 punct .,be
R5241 T18415 T18416 csubj Determining,is
R5242 T18417 T18415 prep between,Determining
R5243 T18418 T18419 det these,possibilities
R5244 T18419 T18417 pobj possibilities,between
R5245 T18420 T18419 nummod two,possibilities
R5246 T18421 T18416 neg not,is
R5247 T18422 T18416 acomp easy,is
R5248 T18423 T18416 punct ", ",is
R5249 T18424 T18425 mark as,is
R5250 T18425 T18416 advcl is,is
R5251 T18426 T18427 compound E2f,immunostaining
R5252 T18427 T18425 nsubj immunostaining,is
R5253 T18428 T18427 prep in,immunostaining
R5254 T18429 T18430 compound mouse,tissues
R5255 T18430 T18428 pobj tissues,in
R5256 T18431 T18425 acomp problematic,is
R5257 T18432 T18416 punct .,is
R5258 T18434 T18435 nsubj We,solve
R5259 T18436 T18435 aux did,solve
R5260 T18437 T18435 neg not,solve
R5261 T18438 T18439 det this,issue
R5262 T18439 T18435 dobj issue,solve
R5263 T18440 T18435 prep for,solve
R5264 T18441 T18440 pobj E2f1,for
R5265 T18442 T18441 cc or,E2f1
R5266 T18443 T18441 conj E2f2,E2f1
R5267 T18444 T18435 punct ", ",solve
R5268 T18445 T18435 cc but,solve
R5269 T18446 T18435 conj used,solve
R5270 T18447 T18448 det a,protocol
R5271 T18448 T18446 dobj protocol,used
R5272 T18449 T18448 amod modified,protocol
R5273 T18450 T18451 punct [,50
R5274 T18451 T18448 parataxis 50,protocol
R5275 T18452 T18451 punct ],50
R5276 T18453 T18454 aux to,track
R5277 T18454 T18446 advcl track,used
R5278 T18455 T18454 advmod successfully,track
R5279 T18456 T18457 compound E2f3,expression
R5280 T18457 T18454 dobj expression,track
R5281 T18458 T18459 punct (,Figure
R5282 T18459 T18454 parataxis Figure,track
R5283 T18460 T18459 nummod 5,Figure
R5284 T18461 T18459 punct ),Figure
R5285 T18462 T18435 punct .,solve
R5286 T18464 T18465 prep At,detected
R5287 T18466 T18464 pobj P0,At
R5288 T18467 T18465 punct ", ",detected
R5289 T18468 T18465 nsubjpass E2f3,detected
R5290 T18469 T18465 auxpass was,detected
R5291 T18470 T18465 prep in,detected
R5292 T18471 T18470 pobj RPCs,in
R5293 T18472 T18465 punct ", ",detected
R5294 T18473 T18465 advcl consistent,detected
R5295 T18474 T18473 prep with,consistent
R5296 T18475 T18476 det a,role
R5297 T18476 T18474 pobj role,with
R5298 T18477 T18476 amod putative,role
R5299 T18478 T18476 prep in,role
R5300 T18479 T18480 amod normal,proliferation
R5301 T18480 T18478 pobj proliferation,in
R5302 T18481 T18482 punct (,5A
R5303 T18482 T18465 parataxis 5A,detected
R5304 T18483 T18482 compound Figure,5A
R5305 T18484 T18482 punct ),5A
R5306 T18485 T18465 punct .,detected
R5307 T18487 T18488 det The,signal
R5308 T18488 T18489 nsubj signal,was
R5309 T18490 T18489 acomp specific,was
R5310 T18491 T18492 mark as,was
R5311 T18492 T18489 advcl was,was
R5312 T18493 T18492 nsubj it,was
R5313 T18494 T18492 acomp absent,was
R5314 T18495 T18492 prep in,was
R5315 T18496 T18497 det the,retina
R5316 T18497 T18495 pobj retina,in
R5317 T18498 T18499 nmod E2f3,KO
R5318 T18499 T18497 nmod KO,retina
R5319 T18500 T18497 amod peripheral,retina
R5320 T18501 T18502 punct (,5A
R5321 T18502 T18492 parataxis 5A,was
R5322 T18503 T18502 compound Figure,5A
R5323 T18504 T18502 punct ),5A
R5324 T18505 T18489 punct .,was
R5325 T18507 T18508 mark As,differentiated
R5326 T18508 T18511 advcl differentiated,dropped
R5327 T18509 T18510 det the,retina
R5328 T18510 T18508 nsubj retina,differentiated
R5329 T18512 T18508 cc and,differentiated
R5330 T18513 T18514 compound RPC,division
R5331 T18514 T18515 nsubj division,diminished
R5332 T18515 T18508 conj diminished,differentiated
R5333 T18516 T18511 punct ", ",dropped
R5334 T18517 T18518 det the,number
R5335 T18518 T18511 nsubj number,dropped
R5336 T18519 T18518 prep of,number
R5337 T18520 T18521 nmod E2f3,cells
R5338 T18521 T18519 pobj cells,of
R5339 T18522 T18520 punct +,E2f3
R5340 T18523 T18511 advmod also,dropped
R5341 T18524 T18511 punct ", ",dropped
R5342 T18525 T18511 cc and,dropped
R5343 T18526 T18527 prep by,expressed
R5344 T18527 T18511 conj expressed,dropped
R5345 T18528 T18526 pobj P8,by
R5346 T18529 T18527 punct ", ",expressed
R5347 T18530 T18531 advmod when,is
R5348 T18531 T18527 advcl is,expressed
R5349 T18532 T18531 nsubj division,is
R5350 T18533 T18534 advmod virtually,over
R5351 T18534 T18531 advmod over,is
R5352 T18535 T18527 punct ", ",expressed
R5353 T18536 T18537 advmod only,subset
R5354 T18537 T18527 nsubj subset,expressed
R5355 T18538 T18537 det a,subset
R5356 T18539 T18537 prep of,subset
R5357 T18540 T18541 amod post-mitotic,cells
R5358 T18541 T18539 pobj cells,of
R5359 T18542 T18537 prep in,subset
R5360 T18543 T18544 det the,retina
R5361 T18544 T18542 pobj retina,in
R5362 T18545 T18544 amod inner,retina
R5363 T18546 T18527 dobj E2f3,expressed
R5364 T18547 T18548 punct (,5A
R5365 T18548 T18527 parataxis 5A,expressed
R5366 T18549 T18548 compound Figure,5A
R5367 T18550 T18548 punct ),5A
R5368 T18551 T18527 punct .,expressed
R5369 T18553 T18554 prep By,detected
R5370 T18555 T18553 pobj P18,By
R5371 T18556 T18554 punct ", ",detected
R5372 T18557 T18554 nsubjpass E2f3,detected
R5373 T18558 T18554 auxpass was,detected
R5374 T18559 T18554 advmod also,detected
R5375 T18560 T18554 prep in,detected
R5376 T18561 T18562 nummod two,tracks
R5377 T18562 T18560 pobj tracks,in
R5378 T18563 T18562 prep in,tracks
R5379 T18564 T18565 det the,IPL
R5380 T18565 T18563 pobj IPL,in
R5381 T18566 T18567 punct (,5A
R5382 T18567 T18562 parataxis 5A,tracks
R5383 T18568 T18567 compound Figure,5A
R5384 T18569 T18567 cc and,5A
R5385 T18570 T18567 conj 5B,5A
R5386 T18571 T18567 punct ),5A
R5387 T18572 T18554 punct ", ",detected
R5388 T18573 T18554 advcl reminiscent,detected
R5389 T18574 T18573 prep of,reminiscent
R5390 T18575 T18576 compound SAC,markers
R5391 T18576 T18574 pobj markers,of
R5392 T18577 T18578 amod such,as
R5393 T18578 T18576 prep as,markers
R5394 T18579 T18578 pobj Chat,as
R5395 T18580 T18579 cc and,Chat
R5396 T18581 T18579 conj Slc18a3,Chat
R5397 T18582 T18583 punct (,3
R5398 T18583 T18554 parataxis 3,detected
R5399 T18584 T18583 advmod c.f.,3
R5400 T18585 T18583 nmod Figures,3
R5401 T18586 T18583 cc and,3
R5402 T18587 T18583 conj 4,3
R5403 T18588 T18583 punct ),3
R5404 T18589 T18554 punct .,detected
R5405 T18591 T18592 det This,staining
R5406 T18592 T18595 nsubj staining,was
R5407 T18593 T18592 amod cytoplasmic,staining
R5408 T18594 T18592 compound E2f3,staining
R5409 T18596 T18595 advmod also,was
R5410 T18597 T18595 acomp specific,was
R5411 T18598 T18595 punct ", ",was
R5412 T18599 T18600 mark as,was
R5413 T18600 T18595 advcl was,was
R5414 T18601 T18600 nsubj it,was
R5415 T18602 T18600 acomp absent,was
R5416 T18603 T18600 prep in,was
R5417 T18604 T18605 det the,retina
R5418 T18605 T18603 pobj retina,in
R5419 T18606 T18607 nmod E2f3,KO
R5420 T18607 T18605 nmod KO,retina
R5421 T18608 T18605 amod peripheral,retina
R5422 T18609 T18605 prep of,retina
R5423 T18610 T18611 nmod α,Cre
R5424 T18611 T18613 nmod Cre,mice
R5425 T18612 T18611 punct -,Cre
R5426 T18613 T18609 pobj mice,of
R5427 T18614 T18611 punct ;,Cre
R5428 T18615 T18616 compound E2f3loxP,loxP
R5429 T18616 T18611 appos loxP,Cre
R5430 T18617 T18616 punct /,loxP
R5431 T18618 T18619 punct (,5A
R5432 T18619 T18595 parataxis 5A,was
R5433 T18620 T18619 compound Figure,5A
R5434 T18621 T18619 punct ),5A
R5435 T18622 T18595 punct .,was
R5436 T18624 T18625 advmod Indeed,confirmed
R5437 T18626 T18625 punct ", ",confirmed
R5438 T18627 T18628 amod double,labelling
R5439 T18628 T18625 nsubj labelling,confirmed
R5440 T18629 T18628 prep with,labelling
R5441 T18630 T18629 pobj E2f3,with
R5442 T18631 T18632 punct (,red
R5443 T18632 T18630 parataxis red,E2f3
R5444 T18633 T18632 punct ),red
R5445 T18634 T18630 cc and,E2f3
R5446 T18635 T18630 conj Chat,E2f3
R5447 T18636 T18635 cc plus,Chat
R5448 T18637 T18635 conj Slc18a3,Chat
R5449 T18638 T18639 punct (,green
R5450 T18639 T18637 parataxis green,Slc18a3
R5451 T18640 T18639 punct ),green
R5452 T18641 T18642 mark that,is
R5453 T18642 T18625 ccomp is,confirmed
R5454 T18643 T18642 nsubj E2f3,is
R5455 T18644 T18642 acomp present,is
R5456 T18645 T18642 prep in,is
R5457 T18646 T18647 preconj both,soma
R5458 T18647 T18645 pobj soma,in
R5459 T18648 T18647 compound SAC,soma
R5460 T18649 T18647 cc and,soma
R5461 T18650 T18647 conj dendrites,soma
R5462 T18651 T18652 punct (,5B
R5463 T18652 T18625 parataxis 5B,confirmed
R5464 T18653 T18652 compound Figure,5B
R5465 T18654 T18652 punct ),5B
R5466 T18655 T18625 punct .,confirmed
R5467 T18657 T18658 compound Rb,protein
R5468 T18658 T18659 nsubjpass protein,detected
R5469 T18660 T18659 auxpass was,detected
R5470 T18661 T18659 advmod also,detected
R5471 T18662 T18659 prep in,detected
R5472 T18663 T18664 det the,retina
R5473 T18664 T18662 pobj retina,in
R5474 T18665 T18664 amod inner,retina
R5475 T18666 T18667 punct (,5A
R5476 T18667 T18659 parataxis 5A,detected
R5477 T18668 T18667 compound Figure,5A
R5478 T18669 T18667 punct ),5A
R5479 T18670 T18659 punct ", ",detected
R5480 T18671 T18659 cc and,detected
R5481 T18672 T18659 conj showed,detected
R5482 T18673 T18674 det a,distribution
R5483 T18674 T18672 dobj distribution,showed
R5484 T18675 T18674 amod similar,distribution
R5485 T18676 T18674 prep as,distribution
R5486 T18677 T18676 pobj E2f3,as
R5487 T18678 T18672 prep in,showed
R5488 T18679 T18678 pobj SACs,in
R5489 T18680 T18681 punct (,5B
R5490 T18681 T18672 parataxis 5B,showed
R5491 T18682 T18681 compound Figure,5B
R5492 T18683 T18681 punct ),5B
R5493 T18684 T18672 punct ", ",showed
R5494 T18685 T18672 cc and,showed
R5495 T18686 T18672 conj was,showed
R5496 T18687 T18686 advmod also,was
R5497 T18688 T18686 acomp present,was
R5498 T18689 T18686 prep in,was
R5499 T18690 T18691 amod mature,cells
R5500 T18691 T18689 pobj cells,in
R5501 T18692 T18691 compound ganglion,cells
R5502 T18693 T18691 cc and,cells
R5503 T18694 T18695 compound Müller,cells
R5504 T18695 T18691 conj cells,cells
R5505 T18696 T18697 mark as,reported
R5506 T18697 T18686 advcl reported,was
R5507 T18698 T18699 punct [,51
R5508 T18699 T18697 parataxis 51,reported
R5509 T18700 T18699 punct ],51
R5510 T18701 T18659 punct .,detected
R5511 T18703 T18704 compound Rb,staining
R5512 T18704 T18705 nsubj staining,was
R5513 T18706 T18704 prep in,staining
R5514 T18707 T18708 compound SAC,processes
R5515 T18708 T18706 pobj processes,in
R5516 T18709 T18705 acomp specific,was
R5517 T18710 T18711 mark as,was
R5518 T18711 T18705 advcl was,was
R5519 T18712 T18711 nsubj it,was
R5520 T18713 T18711 acomp absent,was
R5521 T18714 T18711 prep in,was
R5522 T18715 T18716 det the,retina
R5523 T18716 T18714 pobj retina,in
R5524 T18717 T18716 amod peripheral,retina
R5525 T18718 T18716 prep of,retina
R5526 T18719 T18720 nmod αCre,loxP
R5527 T18720 T18724 compound loxP,mice
R5528 T18721 T18720 punct ;,loxP
R5529 T18722 T18720 compound RbloxP,loxP
R5530 T18723 T18720 punct /,loxP
R5531 T18724 T18718 pobj mice,of
R5532 T18725 T18726 punct (,5A
R5533 T18726 T18705 parataxis 5A,was
R5534 T18727 T18726 compound Figure,5A
R5535 T18728 T18726 punct ),5A
R5536 T18729 T18705 punct .,was
R5537 T18731 T18732 det These,data
R5538 T18732 T18733 nsubj data,suggest
R5539 T18734 T18735 mark that,colocalize
R5540 T18735 T18733 advcl colocalize,suggest
R5541 T18736 T18735 nsubj Rb,colocalize
R5542 T18737 T18736 cc and,Rb
R5543 T18738 T18736 conj E2f3,Rb
R5544 T18739 T18735 prep in,colocalize
R5545 T18740 T18739 pobj SACs,in
R5546 T18741 T18735 cc and,colocalize
R5547 T18742 T18743 mark that,triggers
R5548 T18743 T18735 conj triggers,colocalize
R5549 T18744 T18743 nsubj E2f3,triggers
R5550 T18745 T18743 dobj defects,triggers
R5551 T18746 T18745 prep in,defects
R5552 T18747 T18748 compound SAC,differentiation
R5553 T18748 T18746 pobj differentiation,in
R5554 T18749 T18750 mark because,expressed
R5555 T18750 T18743 advcl expressed,triggers
R5556 T18751 T18750 nsubjpass it,expressed
R5557 T18752 T18750 auxpass is,expressed
R5558 T18753 T18750 advmod specifically,expressed
R5559 T18754 T18750 prep in,expressed
R5560 T18755 T18756 det these,neurons
R5561 T18756 T18754 pobj neurons,in
R5562 T18757 T18756 amod retinal,neurons
R5563 T18758 T18733 punct .,suggest
R5564 T18760 T18761 nsubj We,found
R5565 T18762 T18761 advmod also,found
R5566 T18763 T18764 mark that,is
R5567 T18764 T18761 ccomp is,found
R5568 T18765 T18764 nsubj E2f3,is
R5569 T18766 T18764 acomp present,is
R5570 T18767 T18764 prep in,is
R5571 T18768 T18769 det a,subset
R5572 T18769 T18767 pobj subset,in
R5573 T18770 T18769 amod specific,subset
R5574 T18771 T18769 prep of,subset
R5575 T18772 T18773 amod mature,neurons
R5576 T18773 T18771 pobj neurons,of
R5577 T18774 T18769 prep in,subset
R5578 T18775 T18776 amod various,regions
R5579 T18776 T18774 pobj regions,in
R5580 T18777 T18776 compound brain,regions
R5581 T18778 T18779 punct (,shown
R5582 T18779 T18764 parataxis shown,is
R5583 T18780 T18779 nsubj data,shown
R5584 T18781 T18779 neg not,shown
R5585 T18782 T18779 punct ),shown
R5586 T18783 T18761 punct .,found
R5587 T18785 T18786 prep For,colocalized
R5588 T18787 T18785 pobj example,For
R5589 T18788 T18786 punct ", ",colocalized
R5590 T18789 T18786 prep in,colocalized
R5591 T18790 T18791 det the,amygdala
R5592 T18791 T18789 pobj amygdala,in
R5593 T18792 T18791 compound P20,amygdala
R5594 T18793 T18786 punct ", ",colocalized
R5595 T18794 T18786 nsubj E2f3,colocalized
R5596 T18795 T18786 prep with,colocalized
R5597 T18796 T18797 det the,markers
R5598 T18797 T18795 pobj markers,with
R5599 T18798 T18797 amod general,markers
R5600 T18799 T18797 amod neuronal,markers
R5601 T18800 T18797 appos Mtap2,markers
R5602 T18801 T18800 cc and,Mtap2
R5603 T18802 T18800 conj Mecp2,Mtap2
R5604 T18803 T18804 punct [,52
R5605 T18804 T18797 parataxis 52,markers
R5606 T18805 T18804 punct ],52
R5607 T18806 T18795 punct ", ",with
R5608 T18807 T18795 cc but,with
R5609 T18808 T18807 neg not,but
R5610 T18809 T18795 conj with,with
R5611 T18810 T18809 pobj Calb2,with
R5612 T18811 T18810 punct ", ",Calb2
R5613 T18812 T18813 dep which,marks
R5614 T18813 T18810 relcl marks,Calb2
R5615 T18814 T18815 det a,subset
R5616 T18815 T18813 dobj subset,marks
R5617 T18816 T18815 prep of,subset
R5618 T18817 T18816 pobj neurons,of
R5619 T18818 T18809 punct ", ",with
R5620 T18819 T18809 cc or,with
R5621 T18820 T18809 conj with,with
R5622 T18821 T18822 det the,marker
R5623 T18822 T18820 pobj marker,with
R5624 T18823 T18822 amod glial,marker
R5625 T18824 T18822 appos Gfap,marker
R5626 T18825 T18826 punct (,shown
R5627 T18826 T18786 parataxis shown,colocalized
R5628 T18827 T18826 nsubj data,shown
R5629 T18828 T18826 neg not,shown
R5630 T18829 T18826 punct ),shown
R5631 T18830 T18786 punct .,colocalized
R5632 T18832 T18833 prep Unlike,coexpressed
R5633 T18834 T18832 prep in,Unlike
R5634 T18835 T18836 amod retinal,SACs
R5635 T18836 T18834 pobj SACs,in
R5636 T18837 T18833 punct ", ",coexpressed
R5637 T18838 T18833 nsubjpass E2f3,coexpressed
R5638 T18839 T18833 auxpass was,coexpressed
R5639 T18840 T18833 neg not,coexpressed
R5640 T18841 T18833 prep in,coexpressed
R5641 T18842 T18843 nmod Chat,neurons
R5642 T18843 T18841 pobj neurons,in
R5643 T18844 T18842 punct +,Chat
R5644 T18845 T18842 cc or,Chat
R5645 T18846 T18842 conj Slc18a3,Chat
R5646 T18847 T18846 punct +,Slc18a3
R5647 T18848 T18843 amod cholinergic,neurons
R5648 T18849 T18843 acl located,neurons
R5649 T18850 T18849 prep in,located
R5650 T18851 T18852 amod various,regions
R5651 T18852 T18850 pobj regions,in
R5652 T18853 T18852 prep of,regions
R5653 T18854 T18855 det the,brain
R5654 T18855 T18853 pobj brain,of
R5655 T18856 T18855 cc and,brain
R5656 T18857 T18858 amod spinal,cord
R5657 T18858 T18855 conj cord,brain
R5658 T18859 T18860 punct (,shown
R5659 T18860 T18833 parataxis shown,coexpressed
R5660 T18861 T18860 nsubj data,shown
R5661 T18862 T18860 neg not,shown
R5662 T18863 T18860 punct ),shown
R5663 T18864 T18833 punct .,coexpressed
R5664 T18866 T18867 prep In,detect
R5665 T18868 T18866 pobj agreement,In
R5666 T18869 T18867 punct ", ",detect
R5667 T18870 T18867 nsubj we,detect
R5668 T18871 T18867 aux could,detect
R5669 T18872 T18867 neg not,detect
R5670 T18873 T18867 dobj defects,detect
R5671 T18874 T18873 prep in,defects
R5672 T18875 T18876 amod cholinergic,neurons
R5673 T18876 T18874 pobj neurons,in
R5674 T18877 T18878 compound Rb,KO
R5675 T18878 T18876 compound KO,neurons
R5676 T18879 T18876 prep in,neurons
R5677 T18880 T18881 det the,forebrain
R5678 T18881 T18879 pobj forebrain,in
R5679 T18882 T18881 amod developing,forebrain
R5680 T18883 T18867 punct ", ",detect
R5681 T18884 T18867 cc but,detect
R5682 T18885 T18886 amod other,neurons
R5683 T18886 T18889 nsubj neurons,showed
R5684 T18887 T18888 compound Rb,KO
R5685 T18888 T18886 compound KO,neurons
R5686 T18889 T18867 conj showed,detect
R5687 T18890 T18886 prep in,neurons
R5688 T18891 T18892 det this,region
R5689 T18892 T18890 pobj region,in
R5690 T18893 T18894 compound differentiation,defects
R5691 T18894 T18889 dobj defects,showed
R5692 T18895 T18896 dep that,rescued
R5693 T18896 T18894 relcl rescued,defects
R5694 T18897 T18896 auxpass were,rescued
R5695 T18898 T18896 prep by,rescued
R5696 T18899 T18898 pcomp deleting,by
R5697 T18900 T18899 dobj E2f3,deleting
R5698 T18901 T18902 punct [,53
R5699 T18902 T18889 parataxis 53,showed
R5700 T18903 T18902 punct ],53
R5701 T18904 T18889 punct .,showed
R5702 T18906 T18907 advmod Together,suggest
R5703 T18908 T18907 punct ", ",suggest
R5704 T18909 T18910 det these,results
R5705 T18910 T18907 nsubj results,suggest
R5706 T18911 T18912 mark that,is
R5707 T18912 T18907 ccomp is,suggest
R5708 T18913 T18914 det the,mechanism
R5709 T18914 T18912 nsubj mechanism,is
R5710 T18915 T18914 amod common,mechanism
R5711 T18916 T18917 prep by,promotes
R5712 T18917 T18914 relcl promotes,mechanism
R5713 T18918 T18916 pobj which,by
R5714 T18919 T18917 nsubj Rb,promotes
R5715 T18920 T18921 amod neural,differentiation
R5716 T18921 T18917 dobj differentiation,promotes
R5717 T18922 T18912 prep through,is
R5718 T18923 T18924 compound E2f3,inhibition
R5719 T18924 T18922 pobj inhibition,through
R5720 T18925 T18907 punct .,suggest
R5721 T20216 T20217 amod Distinct,Localization
R5722 T20218 T20217 prep of,Localization
R5723 T20219 T20220 compound E2f3,Isoforms
R5724 T20220 T20218 pobj Isoforms,of
R5725 T20222 T20223 mark As,noted
R5726 T20223 T20224 advcl noted,was
R5727 T20225 T20223 advmod above,noted
R5728 T20226 T20224 punct ", ",was
R5729 T20227 T20228 nmod E2f3,staining
R5730 T20228 T20224 nsubj staining,was
R5731 T20229 T20227 cc and,E2f3
R5732 T20230 T20227 conj Rb,E2f3
R5733 T20231 T20228 prep in,staining
R5734 T20232 T20231 pobj SACs,in
R5735 T20233 T20234 preconj both,nuclear
R5736 T20234 T20224 acomp nuclear,was
R5737 T20235 T20234 cc and,nuclear
R5738 T20236 T20234 conj cytoplasmic,nuclear
R5739 T20237 T20238 punct (,5A
R5740 T20238 T20224 parataxis 5A,was
R5741 T20239 T20238 compound Figure,5A
R5742 T20240 T20238 cc and,5A
R5743 T20241 T20238 conj 5B,5A
R5744 T20242 T20238 punct ),5A
R5745 T20243 T20224 punct .,was
R5746 T20245 T20246 det The,antibody
R5747 T20246 T20247 nsubj antibody,recognizes
R5748 T20248 T20249 dep that,worked
R5749 T20249 T20246 relcl worked,antibody
R5750 T20250 T20249 prep in,worked
R5751 T20251 T20250 pobj immunostaining,in
R5752 T20252 T20253 det a,region
R5753 T20253 T20247 dobj region,recognizes
R5754 T20254 T20255 npadvmod C,terminal
R5755 T20255 T20253 amod terminal,region
R5756 T20256 T20255 punct -,terminal
R5757 T20257 T20247 cc and,recognizes
R5758 T20258 T20259 advmod thus,distinguish
R5759 T20259 T20247 conj distinguish,recognizes
R5760 T20260 T20259 punct ", ",distinguish
R5761 T20261 T20259 aux does,distinguish
R5762 T20262 T20259 neg not,distinguish
R5763 T20263 T20264 compound a,b
R5764 T20264 T20266 compound b,isoforms
R5765 T20265 T20264 punct /,b
R5766 T20266 T20259 dobj isoforms,distinguish
R5767 T20267 T20247 punct .,recognizes
R5768 T20269 T20270 prep To,determined
R5769 T20271 T20272 poss our,knowledge
R5770 T20272 T20269 pobj knowledge,To
R5771 T20273 T20270 punct ", ",determined
R5772 T20274 T20275 det the,location
R5773 T20275 T20270 nsubjpass location,determined
R5774 T20276 T20275 amod subcellular,location
R5775 T20277 T20275 prep of,location
R5776 T20278 T20279 compound E2f3,isoforms
R5777 T20279 T20277 pobj isoforms,of
R5778 T20280 T20270 aux has,determined
R5779 T20281 T20270 neg not,determined
R5780 T20282 T20270 auxpass been,determined
R5781 T20283 T20270 prep in,determined
R5782 T20284 T20285 det any,type
R5783 T20285 T20283 pobj type,in
R5784 T20286 T20285 compound cell,type
R5785 T20287 T20270 punct .,determined
R5786 T20289 T20290 aux To,verify
R5787 T20290 T20291 advcl verify,analyzed
R5788 T20292 T20293 det the,locations
R5789 T20293 T20290 dobj locations,verify
R5790 T20294 T20293 amod dual,locations
R5791 T20295 T20293 prep of,locations
R5792 T20296 T20295 pobj E2f3,of
R5793 T20297 T20290 cc and,verify
R5794 T20298 T20299 aux to,determine
R5795 T20299 T20290 conj determine,verify
R5796 T20300 T20301 det which,isoforms
R5797 T20301 T20302 dep isoforms,were
R5798 T20302 T20299 ccomp were,determine
R5799 T20303 T20302 acomp present,were
R5800 T20304 T20302 prep in,were
R5801 T20305 T20304 pobj retina,in
R5802 T20306 T20291 punct ", ",analyzed
R5803 T20307 T20291 nsubj we,analyzed
R5804 T20308 T20309 amod nuclear,fractions
R5805 T20309 T20291 dobj fractions,analyzed
R5806 T20310 T20308 cc and,nuclear
R5807 T20311 T20308 conj cytoplasmic,nuclear
R5808 T20312 T20291 prep by,analyzed
R5809 T20313 T20314 compound Western,blot
R5810 T20314 T20312 pobj blot,by
R5811 T20315 T20291 prep at,analyzed
R5812 T20316 T20317 amod different,times
R5813 T20317 T20315 pobj times,at
R5814 T20318 T20317 prep during,times
R5815 T20319 T20318 pobj development,during
R5816 T20320 T20291 punct .,analyzed
R5817 T20322 T20323 nsubj Analysis,detected
R5818 T20324 T20322 prep with,Analysis
R5819 T20325 T20326 det the,antibody
R5820 T20326 T20324 pobj antibody,with
R5821 T20327 T20326 amod pan-E2f3,antibody
R5822 T20328 T20326 punct (,antibody
R5823 T20329 T20326 appos sc,antibody
R5824 T20330 T20329 punct -,sc
R5825 T20331 T20329 nummod 878,sc
R5826 T20332 T20333 punct ", ",Biotechnology
R5827 T20333 T20326 parataxis Biotechnology,antibody
R5828 T20334 T20335 compound Santa,Cruz
R5829 T20335 T20333 compound Cruz,Biotechnology
R5830 T20336 T20333 punct ),Biotechnology
R5831 T20337 T20338 det a,species
R5832 T20338 T20323 dobj species,detected
R5833 T20339 T20340 nummod 55,kD
R5834 T20340 T20338 compound kD,species
R5835 T20341 T20340 punct -,kD
R5836 T20342 T20338 compound E2f3a,species
R5837 T20343 T20338 cc and,species
R5838 T20344 T20345 det a,polypeptide
R5839 T20345 T20338 conj polypeptide,species
R5840 T20346 T20347 nummod 40,kD
R5841 T20347 T20345 compound kD,polypeptide
R5842 T20348 T20347 punct -,kD
R5843 T20349 T20345 compound E2f3b,polypeptide
R5844 T20350 T20351 punct (,Figure
R5845 T20351 T20323 parataxis Figure,detected
R5846 T20352 T20351 nummod 6,Figure
R5847 T20353 T20351 punct ),Figure
R5848 T20354 T20323 punct .,detected
R5849 T20356 T20357 aux To,confirm
R5850 T20357 T20358 advcl confirm,exploited
R5851 T20359 T20360 mark that,was
R5852 T20360 T20357 ccomp was,confirm
R5853 T20361 T20362 det the,species
R5854 T20362 T20360 nsubj species,was
R5855 T20363 T20362 amod upper,species
R5856 T20364 T20362 prep in,species
R5857 T20365 T20366 poss our,lysates
R5858 T20366 T20364 pobj lysates,in
R5859 T20367 T20366 amod retinal,lysates
R5860 T20368 T20360 attr E2f3a,was
R5861 T20369 T20358 punct ", ",exploited
R5862 T20370 T20358 nsubj we,exploited
R5863 T20371 T20372 amod novel,mice
R5864 T20372 T20358 dobj mice,exploited
R5865 T20373 T20374 dep that,lack
R5866 T20374 T20372 relcl lack,mice
R5867 T20375 T20376 compound E2f3,1a
R5868 T20376 T20374 dobj 1a,lack
R5869 T20377 T20376 compound exon,1a
R5870 T20378 T20374 cc and,lack
R5871 T20379 T20380 advmod thus,express
R5872 T20380 T20374 conj express,lack
R5873 T20381 T20380 dobj E2f3b,express
R5874 T20382 T20380 advmod exclusively,express
R5875 T20383 T20384 punct (,R.
R5876 T20384 T20380 meta R.,express
R5877 T20385 T20384 nmod O.,R.
R5878 T20386 T20384 cc and,R.
R5879 T20387 T20384 conj G.,R.
R5880 T20388 T20387 nmod L.,G.
R5881 T20389 T20387 punct ", ",G.
R5882 T20390 T20391 amod unpublished,data
R5883 T20391 T20387 conj data,G.
R5884 T20392 T20391 punct ),data
R5885 T20393 T20358 punct .,exploited
R5886 T20395 T20396 det The,strategy
R5887 T20396 T20398 nsubjpass strategy,discussed
R5888 T20397 T20396 compound genotyping,strategy
R5889 T20399 T20398 auxpass is,discussed
R5890 T20400 T20398 prep in,discussed
R5891 T20401 T20400 pobj detail,in
R5892 T20402 T20398 advmod later,discussed
R5893 T20403 T20398 cc and,discussed
R5894 T20404 T20405 auxpass is,outlined
R5895 T20405 T20398 conj outlined,discussed
R5896 T20406 T20405 prep in,outlined
R5897 T20407 T20408 compound Figure,7A
R5898 T20408 T20406 pobj 7A,in
R5899 T20409 T20398 punct .,discussed
R5900 T20411 T20412 compound Western,analysis
R5901 T20412 T20413 nsubj analysis,confirmed
R5902 T20414 T20415 mark that,was
R5903 T20415 T20413 ccomp was,confirmed
R5904 T20416 T20417 det the,band
R5905 T20417 T20415 nsubj band,was
R5906 T20418 T20417 amod upper,band
R5907 T20419 T20415 acomp absent,was
R5908 T20420 T20415 prep in,was
R5909 T20421 T20422 nmod E2f3a,mice
R5910 T20422 T20420 pobj mice,in
R5911 T20423 T20421 punct −,E2f3a
R5912 T20424 T20421 punct /,E2f3a
R5913 T20425 T20421 punct −,E2f3a
R5914 T20426 T20427 punct (,S8
R5915 T20427 T20413 parataxis S8,confirmed
R5916 T20428 T20427 nmod Figures,S8
R5917 T20429 T20427 nummod 6,S8
R5918 T20430 T20427 cc and,S8
R5919 T20431 T20427 punct ),S8
R5920 T20432 T20413 punct .,confirmed
R5921 T20434 T20435 advcl Consistent,was
R5922 T20436 T20434 prep with,Consistent
R5923 T20437 T20438 det the,drop
R5924 T20438 T20436 pobj drop,with
R5925 T20439 T20438 prep in,drop
R5926 T20440 T20441 npadvmod E2f3,expressing
R5927 T20441 T20443 amod expressing,cells
R5928 T20442 T20441 punct -,expressing
R5929 T20443 T20439 pobj cells,in
R5930 T20444 T20438 prep during,drop
R5931 T20445 T20446 nmod WT,maturation
R5932 T20446 T20444 pobj maturation,during
R5933 T20447 T20446 amod retinal,maturation
R5934 T20448 T20449 punct (,5A
R5935 T20449 T20438 parataxis 5A,drop
R5936 T20450 T20449 compound Figure,5A
R5937 T20451 T20449 punct ),5A
R5938 T20452 T20435 punct ", ",was
R5939 T20453 T20454 det the,amount
R5940 T20454 T20435 nsubj amount,was
R5941 T20455 T20454 amod total,amount
R5942 T20456 T20454 prep of,amount
R5943 T20457 T20456 pobj E2f3a,of
R5944 T20458 T20435 acomp less,was
R5945 T20459 T20435 prep at,was
R5946 T20460 T20459 pobj P18,at
R5947 T20461 T20435 prep compared,was
R5948 T20462 T20461 prep to,compared
R5949 T20463 T20462 pobj P0,to
R5950 T20464 T20465 punct (,Figure
R5951 T20465 T20435 parataxis Figure,was
R5952 T20466 T20465 nummod 6,Figure
R5953 T20467 T20465 punct ),Figure
R5954 T20468 T20435 punct .,was
R5955 T20470 T20471 nsubj E2f3b,was
R5956 T20472 T20471 acomp present,was
R5957 T20473 T20471 prep in,was
R5958 T20474 T20475 amod similar,amounts
R5959 T20475 T20473 pobj amounts,in
R5960 T20476 T20471 prep at,was
R5961 T20477 T20478 det both,points
R5962 T20478 T20476 pobj points,at
R5963 T20479 T20478 compound time,points
R5964 T20480 T20471 punct .,was
R5965 T20482 T20483 prep At,was
R5966 T20484 T20482 pobj P0,At
R5967 T20485 T20484 cc and,P0
R5968 T20486 T20484 conj P18,P0
R5969 T20487 T20483 punct ", ",was
R5970 T20488 T20483 nsubj E2f3a,was
R5971 T20489 T20483 acomp present,was
R5972 T20490 T20483 prep in,was
R5973 T20491 T20492 preconj both,nuclear
R5974 T20492 T20493 amod nuclear,fractions
R5975 T20493 T20490 pobj fractions,in
R5976 T20494 T20492 cc and,nuclear
R5977 T20495 T20492 conj cytoplasmic,nuclear
R5978 T20496 T20483 punct ", ",was
R5979 T20497 T20483 cc but,was
R5980 T20498 T20499 prep in,was
R5981 T20499 T20483 conj was,was
R5982 T20500 T20501 amod marked,contrast
R5983 T20501 T20498 pobj contrast,in
R5984 T20502 T20499 punct ", ",was
R5985 T20503 T20499 nsubj E2f3b,was
R5986 T20504 T20505 advmod exclusively,nuclear
R5987 T20505 T20499 acomp nuclear,was
R5988 T20506 T20499 prep at,was
R5989 T20507 T20508 det both,times
R5990 T20508 T20506 pobj times,at
R5991 T20509 T20510 punct (,Figure
R5992 T20510 T20499 parataxis Figure,was
R5993 T20511 T20510 nummod 6,Figure
R5994 T20512 T20510 punct ),Figure
R5995 T20513 T20499 punct .,was
R5996 T20515 T20516 nummod Two,bands
R5997 T20516 T20520 nsubjpass bands,detected
R5998 T20517 T20518 advmod closely,migrating
R5999 T20518 T20516 amod migrating,bands
R6000 T20519 T20516 compound E2f3a,bands
R6001 T20521 T20520 auxpass were,detected
R6002 T20522 T20520 punct ", ",detected
R6003 T20523 T20524 advmod more,clearly
R6004 T20524 T20525 advmod clearly,evident
R6005 T20525 T20520 advcl evident,detected
R6006 T20526 T20525 prep at,evident
R6007 T20527 T20526 pobj P18,at
R6008 T20528 T20520 punct ", ",detected
R6009 T20529 T20530 prep of,was
R6010 T20530 T20520 ccomp was,detected
R6011 T20531 T20529 pobj which,of
R6012 T20532 T20533 det the,species
R6013 T20533 T20530 nsubj species,was
R6014 T20534 T20533 advmod faster,species
R6015 T20535 T20533 amod migrating,species
R6016 T20536 T20530 acomp dominant,was
R6017 T20537 T20530 prep in,was
R6018 T20538 T20537 amod nuclear,in
R6019 T20539 T20530 cc and,was
R6020 T20540 T20541 det the,species
R6021 T20541 T20543 nsubj species,was
R6022 T20542 T20541 amod slower,species
R6023 T20543 T20530 conj was,was
R6024 T20544 T20543 acomp dominant,was
R6025 T20545 T20543 prep in,was
R6026 T20546 T20545 pobj cytoplasm,in
R6027 T20547 T20548 punct (,Figure
R6028 T20548 T20543 parataxis Figure,was
R6029 T20549 T20548 nummod 6,Figure
R6030 T20550 T20548 punct ),Figure
R6031 T20551 T20520 punct .,detected
R6032 T20553 T20554 det The,identity
R6033 T20554 T20555 nsubjpass identity,confirmed
R6034 T20556 T20554 prep of,identity
R6035 T20557 T20556 pobj both,of
R6036 T20558 T20554 prep as,identity
R6037 T20559 T20560 compound E2f3a,species
R6038 T20560 T20558 pobj species,as
R6039 T20561 T20555 auxpass was,confirmed
R6040 T20562 T20555 agent by,confirmed
R6041 T20563 T20564 poss their,absence
R6042 T20564 T20562 pobj absence,by
R6043 T20565 T20564 prep in,absence
R6044 T20566 T20567 det the,retina
R6045 T20567 T20565 pobj retina,in
R6046 T20568 T20567 compound P18,retina
R6047 T20569 T20570 compound E2f3a,KO
R6048 T20570 T20567 compound KO,retina
R6049 T20571 T20572 punct (,S8
R6050 T20572 T20555 parataxis S8,confirmed
R6051 T20573 T20572 compound Figure,S8
R6052 T20574 T20572 punct ),S8
R6053 T20575 T20555 punct .,confirmed
R6054 T20577 T20578 nsubj Analysis,showed
R6055 T20579 T20577 prep of,Analysis
R6056 T20580 T20579 pobj Pou4f2,of
R6057 T20581 T20580 punct ", ",Pou4f2
R6058 T20582 T20583 det a,factor
R6059 T20583 T20580 appos factor,Pou4f2
R6060 T20584 T20583 amod nuclear,factor
R6061 T20585 T20583 compound transcription,factor
R6062 T20586 T20583 acl expressed,factor
R6063 T20587 T20586 prep in,expressed
R6064 T20588 T20589 compound ganglion,cells
R6065 T20589 T20587 pobj cells,in
R6066 T20590 T20578 punct ", ",showed
R6067 T20591 T20592 mark that,contaminated
R6068 T20592 T20578 ccomp contaminated,showed
R6069 T20593 T20594 amod nuclear,proteins
R6070 T20594 T20592 nsubj proteins,contaminated
R6071 T20595 T20592 aux had,contaminated
R6072 T20596 T20592 neg not,contaminated
R6073 T20597 T20598 det the,fraction
R6074 T20598 T20592 dobj fraction,contaminated
R6075 T20599 T20598 amod cytoplasmic,fraction
R6076 T20600 T20578 punct ", ",showed
R6077 T20601 T20578 cc and,showed
R6078 T20602 T20603 nsubj analysis,confirmed
R6079 T20603 T20578 conj confirmed,showed
R6080 T20604 T20602 prep of,analysis
R6081 T20605 T20604 pobj Slc18a3,of
R6082 T20606 T20605 punct ", ",Slc18a3
R6083 T20607 T20608 det a,marker
R6084 T20608 T20605 appos marker,Slc18a3
R6085 T20609 T20608 amod cytoplasmic,marker
R6086 T20610 T20608 compound SAC,marker
R6087 T20611 T20603 punct ", ",confirmed
R6088 T20612 T20613 mark that,occurred
R6089 T20613 T20603 ccomp occurred,confirmed
R6090 T20614 T20615 det the,reverse
R6091 T20615 T20613 nsubj reverse,occurred
R6092 T20616 T20613 aux had,occurred
R6093 T20617 T20613 advmod also,occurred
R6094 T20618 T20613 neg not,occurred
R6095 T20619 T20620 punct (,Figure
R6096 T20620 T20613 parataxis Figure,occurred
R6097 T20621 T20620 nummod 6,Figure
R6098 T20622 T20620 punct ),Figure
R6099 T20623 T20603 punct .,confirmed
R6100 T20625 T20626 det These,data
R6101 T20626 T20627 nsubj data,show
R6102 T20628 T20627 punct ", ",show
R6103 T20629 T20630 prep to,for
R6104 T20630 T20627 prep for,show
R6105 T20631 T20632 poss our,knowledge
R6106 T20632 T20629 pobj knowledge,to
R6107 T20633 T20634 det the,time
R6108 T20634 T20630 pobj time,for
R6109 T20635 T20634 amod first,time
R6110 T20636 T20627 punct ", ",show
R6111 T20637 T20638 mark that,exhibit
R6112 T20638 T20627 ccomp exhibit,show
R6113 T20639 T20638 nsubj E2f3a,exhibit
R6114 T20640 T20639 cc and,E2f3a
R6115 T20641 T20639 conj E2f3b,E2f3a
R6116 T20642 T20643 amod distinct,patterns
R6117 T20643 T20638 dobj patterns,exhibit
R6118 T20644 T20643 prep of,patterns
R6119 T20645 T20646 amod subcellular,distribution
R6120 T20646 T20644 pobj distribution,of
R6121 T20647 T20638 punct ", ",exhibit
R6122 T20648 T20638 cc and,exhibit
R6123 T20649 T20638 conj raise,exhibit
R6124 T20650 T20651 det the,possibility
R6125 T20651 T20649 dobj possibility,raise
R6126 T20652 T20653 mark that,regulated
R6127 T20653 T20651 acl regulated,possibility
R6128 T20654 T20655 compound E2f3a,localization
R6129 T20655 T20653 nsubjpass localization,regulated
R6130 T20656 T20653 aux may,regulated
R6131 T20657 T20653 auxpass be,regulated
R6132 T20658 T20653 agent by,regulated
R6133 T20659 T20660 advmod as,yet
R6134 T20660 T20661 advmod yet,unknown
R6135 T20661 T20662 amod unknown,modifications
R6136 T20662 T20658 pobj modifications,by
R6137 T20663 T20662 amod post-translational,modifications
R6138 T20664 T20627 punct .,show
R6139 T20666 T20667 nsubj We,examined
R6140 T20668 T20667 advmod also,examined
R6141 T20669 T20670 det the,distribution
R6142 T20670 T20667 dobj distribution,examined
R6143 T20671 T20670 prep of,distribution
R6144 T20672 T20673 amod other,regulators
R6145 T20673 T20671 pobj regulators,of
R6146 T20674 T20675 compound cell,cycle
R6147 T20675 T20673 compound cycle,regulators
R6148 T20676 T20667 prep during,examined
R6149 T20677 T20678 amod retinal,development
R6150 T20678 T20676 pobj development,during
R6151 T20679 T20667 punct .,examined
R6152 T20681 T20682 prep Like,was
R6153 T20683 T20681 pobj E2f3a,Like
R6154 T20684 T20682 punct ", ",was
R6155 T20685 T20682 nsubj Rb,was
R6156 T20686 T20682 acomp present,was
R6157 T20687 T20682 prep in,was
R6158 T20688 T20689 preconj both,cytoplasm
R6159 T20689 T20687 pobj cytoplasm,in
R6160 T20690 T20689 det the,cytoplasm
R6161 T20691 T20689 compound WT,cytoplasm
R6162 T20692 T20689 cc and,cytoplasm
R6163 T20693 T20689 conj nucleus,cytoplasm
R6164 T20694 T20682 prep at,was
R6165 T20695 T20694 pobj P0,at
R6166 T20696 T20682 punct ", ",was
R6167 T20697 T20682 cc but,was
R6168 T20698 T20699 prep at,was
R6169 T20699 T20682 conj was,was
R6170 T20700 T20698 pobj P18,at
R6171 T20701 T20699 punct ", ",was
R6172 T20702 T20703 advmod when,increased
R6173 T20703 T20699 advcl increased,was
R6174 T20704 T20705 det the,levels
R6175 T20705 T20703 nsubj levels,increased
R6176 T20706 T20705 prep of,levels
R6177 T20707 T20706 pobj Rb,of
R6178 T20708 T20703 aux had,increased
R6179 T20709 T20699 punct ", ",was
R6180 T20710 T20699 nsubj it,was
R6181 T20711 T20699 advmod primarily,was
R6182 T20712 T20699 acomp nuclear,was
R6183 T20713 T20714 punct (,Figure
R6184 T20714 T20699 parataxis Figure,was
R6185 T20715 T20714 nummod 6,Figure
R6186 T20716 T20714 punct ),Figure
R6187 T20717 T20699 punct .,was
R6188 T20719 T20720 det A,signal
R6189 T20720 T20725 nsubj signal,was
R6190 T20721 T20722 advmod very,faint
R6191 T20722 T20720 amod faint,signal
R6192 T20723 T20720 amod cytoplasmic,signal
R6193 T20724 T20720 compound Rb,signal
R6194 T20726 T20725 acomp evident,was
R6195 T20727 T20725 prep at,was
R6196 T20728 T20727 pobj P18,at
R6197 T20729 T20725 punct ", ",was
R6198 T20730 T20731 dep which,is
R6199 T20731 T20725 advcl is,was
R6200 T20732 T20731 acomp consistent,is
R6201 T20733 T20732 prep with,consistent
R6202 T20734 T20735 compound Rb,staining
R6203 T20735 T20733 pobj staining,with
R6204 T20736 T20735 prep of,staining
R6205 T20737 T20738 compound SAC,processes
R6206 T20738 T20736 pobj processes,of
R6207 T20739 T20740 punct (,5B
R6208 T20740 T20735 parataxis 5B,staining
R6209 T20741 T20740 compound Figure,5B
R6210 T20742 T20740 punct ),5B
R6211 T20743 T20733 punct ", ",with
R6212 T20744 T20733 cc and,with
R6213 T20745 T20733 conj with,with
R6214 T20746 T20747 det the,proportion
R6215 T20747 T20745 pobj proportion,with
R6216 T20748 T20749 advmod very,small
R6217 T20749 T20747 amod small,proportion
R6218 T20750 T20747 prep of,proportion
R6219 T20751 T20750 pobj SACs,of
R6220 T20752 T20747 prep in,proportion
R6221 T20753 T20754 det the,retina
R6222 T20754 T20752 pobj retina,in
R6223 T20755 T20756 punct [,38
R6224 T20756 T20731 parataxis 38,is
R6225 T20757 T20756 punct ],38
R6226 T20758 T20725 punct .,was
R6227 T20760 T20761 nsubjpass E2f1,detected
R6228 T20762 T20761 auxpass was,detected
R6229 T20763 T20761 advmod also,detected
R6230 T20764 T20761 prep in,detected
R6231 T20765 T20766 preconj both,nuclear
R6232 T20766 T20767 amod nuclear,fractions
R6233 T20767 T20764 pobj fractions,in
R6234 T20768 T20766 cc and,nuclear
R6235 T20769 T20766 conj cytoplasmic,nuclear
R6236 T20770 T20761 punct ", ",detected
R6237 T20771 T20772 mark although,was
R6238 T20772 T20761 advcl was,detected
R6239 T20773 T20772 prep unlike,was
R6240 T20774 T20773 pobj E2f3a,unlike
R6241 T20775 T20772 nsubj it,was
R6242 T20776 T20777 advmod predominantly,nuclear
R6243 T20777 T20772 acomp nuclear,was
R6244 T20778 T20779 preconj both,at
R6245 T20779 T20772 prep at,was
R6246 T20780 T20779 pobj P0,at
R6247 T20781 T20780 cc and,P0
R6248 T20782 T20780 conj P18,P0
R6249 T20783 T20784 punct (,Figure
R6250 T20784 T20772 parataxis Figure,was
R6251 T20785 T20784 nummod 6,Figure
R6252 T20786 T20784 punct ),Figure
R6253 T20787 T20761 punct .,detected
R6254 T20789 T20790 det The,partner
R6255 T20790 T20793 nsubj partner,was
R6256 T20791 T20790 compound E2f,partner
R6257 T20792 T20790 compound dimerization,partner
R6258 T20794 T20790 punct ", ",partner
R6259 T20795 T20790 appos Tfdp1,partner
R6260 T20796 T20790 punct ", ",partner
R6261 T20797 T20798 dep which,lacks
R6262 T20798 T20790 relcl lacks,partner
R6263 T20799 T20800 det a,signal
R6264 T20800 T20798 dobj signal,lacks
R6265 T20801 T20802 amod nuclear,localization
R6266 T20802 T20800 compound localization,signal
R6267 T20803 T20804 punct [,54
R6268 T20804 T20798 parataxis 54,lacks
R6269 T20805 T20804 punct ],54
R6270 T20806 T20793 punct ", ",was
R6271 T20807 T20808 advmod primarily,cytoplasmic
R6272 T20808 T20793 acomp cytoplasmic,was
R6273 T20809 T20793 prep at,was
R6274 T20810 T20811 preconj both,P0
R6275 T20811 T20809 pobj P0,at
R6276 T20812 T20811 cc and,P0
R6277 T20813 T20811 conj P18,P0
R6278 T20814 T20793 punct ", ",was
R6279 T20815 T20793 cc and,was
R6280 T20816 T20817 det the,inhibitors
R6281 T20817 T20819 nsubj inhibitors,showed
R6282 T20818 T20817 compound Cdk,inhibitors
R6283 T20819 T20793 conj showed,was
R6284 T20820 T20817 appos Cdkn1a,inhibitors
R6285 T20821 T20820 cc and,Cdkn1a
R6286 T20822 T20820 conj Cdkn1b,Cdkn1a
R6287 T20823 T20824 det a,pattern
R6288 T20824 T20819 dobj pattern,showed
R6289 T20825 T20824 amod similar,pattern
R6290 T20826 T20824 prep of,pattern
R6291 T20827 T20826 pobj distribution,of
R6292 T20828 T20829 punct (,Figure
R6293 T20829 T20819 parataxis Figure,showed
R6294 T20830 T20829 nummod 6,Figure
R6295 T20831 T20829 punct ),Figure
R6296 T20832 T20819 punct .,showed
R6297 T20834 T20835 advmod Thus,showed
R6298 T20836 T20835 punct ", ",showed
R6299 T20837 T20835 prep among,showed
R6300 T20838 T20839 det the,regulators
R6301 T20839 T20837 pobj regulators,among
R6302 T20840 T20841 compound cell,cycle
R6303 T20841 T20839 compound cycle,regulators
R6304 T20842 T20843 nsubj we,examined
R6305 T20843 T20839 advcl examined,regulators
R6306 T20844 T20835 punct ", ",showed
R6307 T20845 T20835 nsubj most,showed
R6308 T20846 T20847 amod bivalent,distribution
R6309 T20847 T20835 dobj distribution,showed
R6310 T20848 T20835 punct ", ",showed
R6311 T20849 T20835 cc and,showed
R6312 T20850 T20851 nsubj E2f3b,was
R6313 T20851 T20835 conj was,showed
R6314 T20852 T20851 acomp unusual,was
R6315 T20853 T20851 prep in,was
R6316 T20854 T20855 poss its,compartmentalization
R6317 T20855 T20853 pobj compartmentalization,in
R6318 T20856 T20857 advmod solely,nuclear
R6319 T20857 T20855 amod nuclear,compartmentalization
R6320 T20858 T20851 punct .,was
R6321 T21488 T21489 nsubj Rb,Regulates
R6322 T21490 T21491 compound SAC,Differentiation
R6323 T21491 T21489 dobj Differentiation,Regulates
R6324 T21492 T21489 prep through,Regulates
R6325 T21493 T21492 pobj E2f3a,through
R6326 T21495 T21496 aux To,test
R6327 T21496 T21497 advcl test,exploited
R6328 T21498 T21499 det which,isoform
R6329 T21499 T21501 dep isoform,is
R6330 T21500 T21499 compound E2f3,isoform
R6331 T21501 T21496 ccomp is,test
R6332 T21502 T21501 acomp responsible,is
R6333 T21503 T21502 prep for,responsible
R6334 T21504 T21505 amod aberrant,differentiation
R6335 T21505 T21503 pobj differentiation,for
R6336 T21506 T21507 compound Rb,KO
R6337 T21507 T21505 compound KO,differentiation
R6338 T21508 T21505 compound SAC,differentiation
R6339 T21509 T21497 nsubj we,exploited
R6340 T21510 T21511 nmod E2f3a,mice
R6341 T21511 T21497 dobj mice,exploited
R6342 T21512 T21510 punct −,E2f3a
R6343 T21513 T21510 punct /,E2f3a
R6344 T21514 T21510 punct −,E2f3a
R6345 T21515 T21516 punct (,7A
R6346 T21516 T21497 parataxis 7A,exploited
R6347 T21517 T21516 compound Figure,7A
R6348 T21518 T21516 punct ),7A
R6349 T21519 T21497 punct .,exploited
R6350 T21521 T21522 det The,strategy
R6351 T21522 T21524 nsubjpass strategy,used
R6352 T21523 T21522 compound genotyping,strategy
R6353 T21525 T21522 acl outlined,strategy
R6354 T21526 T21525 prep in,outlined
R6355 T21527 T21528 compound Figure,7A
R6356 T21528 T21526 pobj 7A,in
R6357 T21529 T21524 auxpass was,used
R6358 T21530 T21531 aux to,distinguish
R6359 T21531 T21524 advcl distinguish,used
R6360 T21532 T21533 det the,alleles
R6361 T21533 T21531 dobj alleles,distinguish
R6362 T21534 T21533 nmod E2f3a,alleles
R6363 T21535 T21534 punct ", ",E2f3a
R6364 T21536 T21534 conj WT,E2f3a
R6365 T21537 T21536 punct ", ",WT
R6366 T21538 T21536 cc and,WT
R6367 T21539 T21536 conj null,WT
R6368 T21540 T21524 punct .,used
R6369 T21542 T21543 amod Reverse,PCR
R6370 T21543 T21545 nsubj PCR,confirmed
R6371 T21544 T21543 compound transcriptase,PCR
R6372 T21546 T21543 punct (,PCR
R6373 T21547 T21548 compound RT,PCR
R6374 T21548 T21543 appos PCR,PCR
R6375 T21549 T21548 punct -,PCR
R6376 T21550 T21545 punct ),confirmed
R6377 T21551 T21552 det the,presence
R6378 T21552 T21545 dobj presence,confirmed
R6379 T21553 T21552 prep of,presence
R6380 T21554 T21555 preconj both,E2f3a
R6381 T21555 T21556 nmod E2f3a,species
R6382 T21556 T21553 pobj species,of
R6383 T21557 T21555 cc and,E2f3a
R6384 T21558 T21555 conj E2f3b,E2f3a
R6385 T21559 T21556 compound RNA,species
R6386 T21560 T21552 prep in,presence
R6387 T21561 T21562 det the,retina
R6388 T21562 T21560 pobj retina,in
R6389 T21563 T21562 amod developing,retina
R6390 T21564 T21562 compound WT,retina
R6391 T21565 T21552 punct ", ",presence
R6392 T21566 T21552 cc and,presence
R6393 T21567 T21568 det the,absence
R6394 T21568 T21552 conj absence,presence
R6395 T21569 T21568 amod specific,absence
R6396 T21570 T21568 prep of,absence
R6397 T21571 T21572 compound E2f3a,RNA
R6398 T21572 T21570 pobj RNA,of
R6399 T21573 T21568 prep in,absence
R6400 T21574 T21575 det the,retina
R6401 T21575 T21573 pobj retina,in
R6402 T21576 T21575 nmod E2f3a,retina
R6403 T21577 T21576 punct −,E2f3a
R6404 T21578 T21576 punct /,E2f3a
R6405 T21579 T21576 punct −,E2f3a
R6406 T21580 T21581 punct (,7B
R6407 T21581 T21545 parataxis 7B,confirmed
R6408 T21582 T21581 compound Figure,7B
R6409 T21583 T21581 punct ),7B
R6410 T21584 T21545 punct .,confirmed
R6411 T21586 T21587 compound E2f3a,protein
R6412 T21587 T21588 nsubj protein,was
R6413 T21589 T21588 acomp absent,was
R6414 T21590 T21588 prep in,was
R6415 T21591 T21592 nmod E2f3a,lysate
R6416 T21592 T21590 pobj lysate,in
R6417 T21593 T21591 punct −,E2f3a
R6418 T21594 T21591 punct /,E2f3a
R6419 T21595 T21591 punct −,E2f3a
R6420 T21596 T21592 amod retinal,lysate
R6421 T21597 T21598 punct (,S8
R6422 T21598 T21588 parataxis S8,was
R6423 T21599 T21598 nmod Figures,S8
R6424 T21600 T21598 nummod 6,S8
R6425 T21601 T21598 cc and,S8
R6426 T21602 T21598 punct ),S8
R6427 T21603 T21588 punct .,was
R6428 T21605 T21606 advmod Importantly,were
R6429 T21607 T21606 punct ", ",were
R6430 T21608 T21609 det the,levels
R6431 T21609 T21606 nsubj levels,were
R6432 T21610 T21609 prep of,levels
R6433 T21611 T21612 compound E2f3b,message
R6434 T21612 T21610 pobj message,of
R6435 T21613 T21606 acomp similar,were
R6436 T21614 T21606 prep in,were
R6437 T21615 T21616 det the,retina
R6438 T21616 T21614 pobj retina,in
R6439 T21617 T21618 nmod Rb,KO
R6440 T21618 T21616 nmod KO,retina
R6441 T21619 T21618 cc and,KO
R6442 T21620 T21621 compound Rb,E2f3a
R6443 T21621 T21623 compound E2f3a,DKO
R6444 T21622 T21621 punct /,E2f3a
R6445 T21623 T21618 conj DKO,KO
R6446 T21624 T21606 punct ", ",were
R6447 T21625 T21606 advcl ruling,were
R6448 T21626 T21625 prt out,ruling
R6449 T21627 T21628 det the,possibility
R6450 T21628 T21625 dobj possibility,ruling
R6451 T21629 T21630 mark that,were
R6452 T21630 T21628 acl were,possibility
R6453 T21631 T21632 det any,effects
R6454 T21632 T21630 nsubj effects,were
R6455 T21633 T21632 prep of,effects
R6456 T21634 T21635 compound E2f3a,deletion
R6457 T21635 T21633 pobj deletion,of
R6458 T21636 T21637 nsubj we,observe
R6459 T21637 T21632 advcl observe,effects
R6460 T21638 T21637 aux might,observe
R6461 T21639 T21630 prep due,were
R6462 T21640 T21639 pcomp to,due
R6463 T21641 T21642 amod down,regulation
R6464 T21642 T21639 pobj regulation,due
R6465 T21643 T21642 punct -,regulation
R6466 T21644 T21642 prep of,regulation
R6467 T21645 T21644 pobj E2f3b,of
R6468 T21646 T21647 punct (,7C
R6469 T21647 T21630 parataxis 7C,were
R6470 T21648 T21647 compound Figure,7C
R6471 T21649 T21647 punct ),7C
R6472 T21650 T21606 punct .,were
R6473 T21652 T21653 advmod Also,were
R6474 T21654 T21653 punct ", ",were
R6475 T21655 T21656 det the,levels
R6476 T21656 T21653 nsubj levels,were
R6477 T21657 T21656 prep of,levels
R6478 T21658 T21659 amod other,E2fs
R6479 T21659 T21657 pobj E2fs,of
R6480 T21660 T21661 det the,same
R6481 T21661 T21653 attr same,were
R6482 T21662 T21661 prep in,same
R6483 T21663 T21664 det the,retina
R6484 T21664 T21662 pobj retina,in
R6485 T21665 T21666 nmod Rb,KO
R6486 T21666 T21664 nmod KO,retina
R6487 T21667 T21666 punct ", ",KO
R6488 T21668 T21669 compound Rb,E2f3
R6489 T21669 T21671 compound E2f3,DKO
R6490 T21670 T21669 punct /,E2f3
R6491 T21671 T21666 conj DKO,KO
R6492 T21672 T21671 punct ", ",DKO
R6493 T21673 T21671 cc and,DKO
R6494 T21674 T21675 compound Rb,E2f3a
R6495 T21675 T21677 compound E2f3a,DKO
R6496 T21676 T21675 punct /,E2f3a
R6497 T21677 T21671 conj DKO,DKO
R6498 T21678 T21653 punct ", ",were
R6499 T21679 T21653 advcl ruling,were
R6500 T21680 T21679 prt out,ruling
R6501 T21681 T21682 det any,effects
R6502 T21682 T21679 dobj effects,ruling
R6503 T21683 T21684 advmod cross,regulatory
R6504 T21684 T21682 amod regulatory,effects
R6505 T21685 T21684 punct -,regulatory
R6506 T21686 T21687 punct (,7C
R6507 T21687 T21653 parataxis 7C,were
R6508 T21688 T21687 compound Figure,7C
R6509 T21689 T21687 punct ),7C
R6510 T21690 T21691 punct [,55
R6511 T21691 T21653 parataxis 55,were
R6512 T21692 T21691 punct ],55
R6513 T21693 T21653 punct .,were
R6514 T21695 T21696 nsubj E2f3a,trigger
R6515 T21697 T21696 aux can,trigger
R6516 T21698 T21699 compound cell,cycle
R6517 T21699 T21700 compound cycle,induction
R6518 T21700 T21696 dobj induction,trigger
R6519 T21701 T21696 punct ", ",trigger
R6520 T21702 T21696 cc but,trigger
R6521 T21703 T21704 mark because,linked
R6522 T21704 T21709 advcl linked,suspected
R6523 T21705 T21706 compound SAC,defects
R6524 T21706 T21704 nsubjpass defects,linked
R6525 T21707 T21704 auxpass are,linked
R6526 T21708 T21704 neg not,linked
R6527 T21709 T21696 conj suspected,trigger
R6528 T21710 T21704 prep to,linked
R6529 T21711 T21712 compound cell,cycle
R6530 T21712 T21713 compound cycle,perturbation
R6531 T21713 T21710 pobj perturbation,to
R6532 T21714 T21715 punct (,3A
R6533 T21715 T21704 parataxis 3A,linked
R6534 T21716 T21715 compound Figures,3A
R6535 T21717 T21715 cc and,3A
R6536 T21718 T21715 conj 4,3A
R6537 T21719 T21715 punct ),3A
R6538 T21720 T21704 punct ", ",linked
R6539 T21721 T21704 cc and,linked
R6540 T21722 T21704 conj in,linked
R6541 T21723 T21722 pobj view,in
R6542 T21724 T21723 prep of,view
R6543 T21725 T21726 det the,association
R6544 T21726 T21724 pobj association,of
R6545 T21727 T21726 amod predominant,association
R6546 T21728 T21726 prep between,association
R6547 T21729 T21728 pobj E2f3b,between
R6548 T21730 T21729 cc and,E2f3b
R6549 T21731 T21729 conj Rb,E2f3b
R6550 T21732 T21726 prep in,association
R6551 T21733 T21734 amod quiescent,cells
R6552 T21734 T21732 pobj cells,in
R6553 T21735 T21736 punct [,19
R6554 T21736 T21726 parataxis 19,association
R6555 T21737 T21736 nummod 16,19
R6556 T21738 T21736 punct ",",19
R6557 T21739 T21736 punct ],19
R6558 T21740 T21709 punct ", ",suspected
R6559 T21741 T21709 nsubj we,suspected
R6560 T21742 T21743 mark that,perturb
R6561 T21743 T21709 ccomp perturb,suspected
R6562 T21744 T21743 nsubj E2f3b,perturb
R6563 T21745 T21743 aux may,perturb
R6564 T21746 T21743 dobj differentiation,perturb
R6565 T21747 T21743 prep in,perturb
R6566 T21748 T21749 compound Rb,KO
R6567 T21749 T21750 compound KO,SACs
R6568 T21750 T21747 pobj SACs,in
R6569 T21751 T21709 punct .,suspected
R6570 T21753 T21754 advmod Unexpectedly,suppressed
R6571 T21755 T21754 punct ", ",suppressed
R6572 T21756 T21754 advmod however,suppressed
R6573 T21757 T21754 punct ", ",suppressed
R6574 T21758 T21759 compound E2f3a,deletion
R6575 T21759 T21754 nsubj deletion,suppressed
R6576 T21760 T21761 det the,defect
R6577 T21761 T21754 dobj defect,suppressed
R6578 T21762 T21763 compound Rb,KO
R6579 T21763 T21761 compound KO,defect
R6580 T21764 T21761 compound SAC,defect
R6581 T21765 T21766 punct (,7D
R6582 T21766 T21754 parataxis 7D,suppressed
R6583 T21767 T21766 compound Figure,7D
R6584 T21768 T21766 punct ),7D
R6585 T21769 T21754 punct .,suppressed
R6586 T21771 T21772 advmod Thus,is
R6587 T21773 T21772 punct ", ",is
R6588 T21774 T21772 advcl separate,is
R6589 T21775 T21774 prep from,separate
R6590 T21776 T21777 poss its,role
R6591 T21777 T21775 pobj role,from
R6592 T21778 T21777 prep in,role
R6593 T21779 T21780 compound cell,cycle
R6594 T21780 T21781 compound cycle,control
R6595 T21781 T21778 pobj control,in
R6596 T21782 T21772 punct ", ",is
R6597 T21783 T21784 compound Rb,regulation
R6598 T21784 T21772 nsubj regulation,is
R6599 T21785 T21784 prep of,regulation
R6600 T21786 T21785 pobj E2f3a,of
R6601 T21787 T21772 acomp critical,is
R6602 T21788 T21789 aux to,ensure
R6603 T21789 T21772 advcl ensure,is
R6604 T21790 T21791 amod proper,differentiation
R6605 T21791 T21789 dobj differentiation,ensure
R6606 T21792 T21791 amod neuronal,differentiation
R6607 T21793 T21772 punct .,is