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PMC:1892049 / 27981-28690 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T13807 0-7 NNP denotes Western
T13808 8-12 NN denotes blot
T13809 13-21 NN denotes analysis
T13810 22-24 IN denotes in
T13811 25-30 NN denotes SCA15
T13812 31-39 NNS denotes patients
T13813 39-40 . denotes .
T13814 40-270 sentence denotes EBV immortalized lymphoblasts from three affected family members who carry the deletion and one family member without the mutation were used as a readily accessible source of protein; all samples came from members of family AUS1.
T13815 41-44 NN denotes EBV
T13817 45-57 VBN denotes immortalized
T13816 58-70 NNS denotes lymphoblasts
T13819 71-75 IN denotes from
T13820 76-81 CD denotes three
T13822 82-90 VBN denotes affected
T13823 91-97 NN denotes family
T13821 98-105 NNS denotes members
T13824 106-109 WP denotes who
T13825 110-115 VBP denotes carry
T13826 116-119 DT denotes the
T13827 120-128 NN denotes deletion
T13828 129-132 CC denotes and
T13829 133-136 CD denotes one
T13831 137-143 NN denotes family
T13830 144-150 NN denotes member
T13832 151-158 IN denotes without
T13833 159-162 DT denotes the
T13834 163-171 NN denotes mutation
T13835 172-176 VBD denotes were
T13818 177-181 VBN denotes used
T13837 182-184 IN denotes as
T13838 185-186 DT denotes a
T13840 187-194 RB denotes readily
T13841 195-205 JJ denotes accessible
T13839 206-212 NN denotes source
T13842 213-215 IN denotes of
T13843 216-223 NN denotes protein
T13844 223-224 : denotes ;
T13845 225-228 DT denotes all
T13846 229-236 NNS denotes samples
T13836 237-241 VBD denotes came
T13847 242-246 IN denotes from
T13848 247-254 NNS denotes members
T13849 255-257 IN denotes of
T13850 258-264 NN denotes family
T13851 265-269 NN denotes AUS1
T13852 269-270 . denotes .
T13853 270-436 sentence denotes Protein extraction was performed using lysis buffer containing 1× TBS, 1% Triton X-100, and a cocktail of protease inhibitors (Roche) with overnight lysis at −80 °C.
T13854 271-278 NN denotes Protein
T13855 279-289 NN denotes extraction
T13857 290-293 VBD denotes was
T13856 294-303 VBN denotes performed
T13858 304-309 VBG denotes using
T13859 310-315 NN denotes lysis
T13860 316-322 NN denotes buffer
T13861 323-333 VBG denotes containing
T13862 334-335 CD denotes 1
T13864 335-336 SYM denotes ×
T13863 337-340 NN denotes TBS
T13865 340-342 , denotes ,
T13866 342-343 CD denotes 1
T13867 343-344 NN denotes %
T13869 345-351 NN denotes Triton
T13868 352-353 NN denotes X
T13870 353-354 HYPH denotes -
T13871 354-357 CD denotes 100
T13872 357-359 , denotes ,
T13873 359-362 CC denotes and
T13874 363-364 DT denotes a
T13875 365-373 NN denotes cocktail
T13876 374-376 IN denotes of
T13877 377-385 NN denotes protease
T13878 386-396 NNS denotes inhibitors
T13879 397-398 -LRB- denotes (
T13880 398-403 NNP denotes Roche
T13881 403-404 -RRB- denotes )
T13882 405-409 IN denotes with
T13883 410-419 JJ denotes overnight
T13884 420-425 NN denotes lysis
T13885 426-428 IN denotes at
T13886 429-430 SYM denotes −
T13887 430-432 CD denotes 80
T13888 433-435 NN denotes °C
T13889 435-436 . denotes .
T13890 436-613 sentence denotes Homogenates were diluted appropriately, mixed with 4× reducing sample buffer, and loaded onto 4%–12% precast gradient gels (NuPAGE, Invitrogen) for SDS-PAGE and immunoblotting.
T13891 437-448 NNS denotes Homogenates
T13893 449-453 VBD denotes were
T13892 454-461 VBN denotes diluted
T13894 462-475 RB denotes appropriately
T13895 475-477 , denotes ,
T13896 477-482 VBN denotes mixed
T13897 483-487 IN denotes with
T13898 488-489 CD denotes 4
T13900 489-490 SYM denotes ×
T13901 491-499 VBG denotes reducing
T13902 500-506 NN denotes sample
T13899 507-513 NN denotes buffer
T13903 513-515 , denotes ,
T13904 515-518 CC denotes and
T13905 519-525 VBN denotes loaded
T13906 526-530 IN denotes onto
T13907 531-532 CD denotes 4
T13908 532-533 NN denotes %
T13910 533-534 SYM denotes –
T13912 534-536 CD denotes 12
T13911 536-537 NN denotes %
T13913 538-545 JJ denotes precast
T13914 546-554 NN denotes gradient
T13909 555-559 NNS denotes gels
T13915 560-561 -LRB- denotes (
T13916 561-567 NN denotes NuPAGE
T13917 567-569 , denotes ,
T13918 569-579 NNP denotes Invitrogen
T13919 579-580 -RRB- denotes )
T13920 581-584 IN denotes for
T13921 585-588 NN denotes SDS
T13923 588-589 HYPH denotes -
T13922 589-593 NN denotes PAGE
T13924 594-597 CC denotes and
T13925 598-612 NN denotes immunoblotting
T13926 612-613 . denotes .
T13927 613-709 sentence denotes The antibodies to ITPR1 (1:1,000) and ACTB (1:5,000) were used as recommended by manufacturers.
T13928 614-617 DT denotes The
T13929 618-628 NNS denotes antibodies
T13931 629-631 IN denotes to
T13932 632-637 NN denotes ITPR1
T13933 638-639 -LRB- denotes (
T13934 639-640 CD denotes 1
T13935 640-641 SYM denotes :
T13936 641-646 CD denotes 1,000
T13937 646-647 -RRB- denotes )
T13938 648-651 CC denotes and
T13939 652-656 NN denotes ACTB
T13940 657-658 -LRB- denotes (
T13941 658-659 CD denotes 1
T13942 659-660 SYM denotes :
T13943 660-665 CD denotes 5,000
T13944 665-666 -RRB- denotes )
T13945 667-671 VBD denotes were
T13930 672-676 VBN denotes used
T13946 677-679 IN denotes as
T13947 680-691 VBN denotes recommended
T13948 692-694 IN denotes by
T13949 695-708 NNS denotes manufacturers
T13950 708-709 . denotes .
R4064 T13807 T13808 compound Western,blot
R4065 T13808 T13809 compound blot,analysis
R4066 T13810 T13809 prep in,analysis
R4067 T13811 T13812 compound SCA15,patients
R4068 T13812 T13810 pobj patients,in
R4069 T13813 T13809 punct .,analysis
R4070 T13815 T13816 nmod EBV,lymphoblasts
R4071 T13816 T13818 nsubjpass lymphoblasts,used
R4072 T13817 T13816 amod immortalized,lymphoblasts
R4073 T13818 T13836 ccomp used,came
R4074 T13819 T13816 prep from,lymphoblasts
R4075 T13820 T13821 nummod three,members
R4076 T13821 T13819 pobj members,from
R4077 T13822 T13821 amod affected,members
R4078 T13823 T13821 compound family,members
R4079 T13824 T13825 dep who,carry
R4080 T13825 T13821 relcl carry,members
R4081 T13826 T13827 det the,deletion
R4082 T13827 T13825 dobj deletion,carry
R4083 T13828 T13821 cc and,members
R4084 T13829 T13830 nummod one,member
R4085 T13830 T13821 conj member,members
R4086 T13831 T13830 compound family,member
R4087 T13832 T13830 prep without,member
R4088 T13833 T13834 det the,mutation
R4089 T13834 T13832 pobj mutation,without
R4090 T13835 T13818 auxpass were,used
R4091 T13837 T13818 prep as,used
R4092 T13838 T13839 det a,source
R4093 T13839 T13837 pobj source,as
R4094 T13840 T13841 advmod readily,accessible
R4095 T13841 T13839 amod accessible,source
R4096 T13842 T13839 prep of,source
R4097 T13843 T13842 pobj protein,of
R4098 T13844 T13836 punct ;,came
R4099 T13845 T13846 det all,samples
R4100 T13846 T13836 nsubj samples,came
R4101 T13847 T13836 prep from,came
R4102 T13848 T13847 pobj members,from
R4103 T13849 T13848 prep of,members
R4104 T13850 T13851 compound family,AUS1
R4105 T13851 T13849 pobj AUS1,of
R4106 T13852 T13836 punct .,came
R4107 T13854 T13855 compound Protein,extraction
R4108 T13855 T13856 nsubjpass extraction,performed
R4109 T13857 T13856 auxpass was,performed
R4110 T13858 T13856 advcl using,performed
R4111 T13859 T13860 compound lysis,buffer
R4112 T13860 T13858 dobj buffer,using
R4113 T13861 T13860 acl containing,buffer
R4114 T13862 T13863 nummod 1,TBS
R4115 T13863 T13861 dobj TBS,containing
R4116 T13864 T13862 punct ×,1
R4117 T13865 T13863 punct ", ",TBS
R4118 T13866 T13867 nummod 1,%
R4119 T13867 T13868 compound %,X
R4120 T13868 T13863 conj X,TBS
R4121 T13869 T13868 compound Triton,X
R4122 T13870 T13868 punct -,X
R4123 T13871 T13868 nummod 100,X
R4124 T13872 T13868 punct ", ",X
R4125 T13873 T13868 cc and,X
R4126 T13874 T13875 det a,cocktail
R4127 T13875 T13868 conj cocktail,X
R4128 T13876 T13875 prep of,cocktail
R4129 T13877 T13878 compound protease,inhibitors
R4130 T13878 T13876 pobj inhibitors,of
R4131 T13879 T13880 punct (,Roche
R4132 T13880 T13878 parataxis Roche,inhibitors
R4133 T13881 T13880 punct ),Roche
R4134 T13882 T13856 prep with,performed
R4135 T13883 T13884 amod overnight,lysis
R4136 T13884 T13882 pobj lysis,with
R4137 T13885 T13856 prep at,performed
R4138 T13886 T13887 punct −,80
R4139 T13887 T13888 nummod 80,°C
R4140 T13888 T13885 pobj °C,at
R4141 T13889 T13856 punct .,performed
R4142 T13891 T13892 nsubjpass Homogenates,diluted
R4143 T13893 T13892 auxpass were,diluted
R4144 T13894 T13892 advmod appropriately,diluted
R4145 T13895 T13892 punct ", ",diluted
R4146 T13896 T13892 conj mixed,diluted
R4147 T13897 T13896 prep with,mixed
R4148 T13898 T13899 nummod 4,buffer
R4149 T13899 T13897 pobj buffer,with
R4150 T13900 T13898 punct ×,4
R4151 T13901 T13899 amod reducing,buffer
R4152 T13902 T13899 compound sample,buffer
R4153 T13903 T13896 punct ", ",mixed
R4154 T13904 T13896 cc and,mixed
R4155 T13905 T13896 conj loaded,mixed
R4156 T13906 T13905 prep onto,loaded
R4157 T13907 T13908 nummod 4,%
R4158 T13908 T13909 nmod %,gels
R4159 T13909 T13906 pobj gels,onto
R4160 T13910 T13911 punct –,%
R4161 T13911 T13908 prep %,%
R4162 T13912 T13911 nummod 12,%
R4163 T13913 T13909 amod precast,gels
R4164 T13914 T13909 compound gradient,gels
R4165 T13915 T13916 punct (,NuPAGE
R4166 T13916 T13909 parataxis NuPAGE,gels
R4167 T13917 T13916 punct ", ",NuPAGE
R4168 T13918 T13916 appos Invitrogen,NuPAGE
R4169 T13919 T13916 punct ),NuPAGE
R4170 T13920 T13905 prep for,loaded
R4171 T13921 T13922 compound SDS,PAGE
R4172 T13922 T13920 pobj PAGE,for
R4173 T13923 T13922 punct -,PAGE
R4174 T13924 T13922 cc and,PAGE
R4175 T13925 T13922 conj immunoblotting,PAGE
R4176 T13926 T13892 punct .,diluted
R4177 T13928 T13929 det The,antibodies
R4178 T13929 T13930 nsubjpass antibodies,used
R4179 T13931 T13929 prep to,antibodies
R4180 T13932 T13931 pobj ITPR1,to
R4181 T13933 T13934 punct (,1
R4182 T13934 T13932 parataxis 1,ITPR1
R4183 T13935 T13936 punct :,"1,000"
R4184 T13936 T13934 prep "1,000",1
R4185 T13937 T13934 punct ),1
R4186 T13938 T13932 cc and,ITPR1
R4187 T13939 T13932 conj ACTB,ITPR1
R4188 T13940 T13941 punct (,1
R4189 T13941 T13939 parataxis 1,ACTB
R4190 T13942 T13943 punct :,"5,000"
R4191 T13943 T13941 prep "5,000",1
R4192 T13944 T13941 punct ),1
R4193 T13945 T13930 auxpass were,used
R4194 T13946 T13947 mark as,recommended
R4195 T13947 T13930 advcl recommended,used
R4196 T13948 T13947 agent by,recommended
R4197 T13949 T13948 pobj manufacturers,by
R4198 T13950 T13930 punct .,used