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PMC:1892049 / 21969-23829 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T10938 0-6 NNS denotes Brains
T10940 7-11 VBD denotes were
T10939 12-20 VBN denotes isolated
T10941 21-25 IN denotes from
T10942 26-28 CD denotes 21
T10944 28-29 HYPH denotes -
T10943 29-30 NN denotes d
T10946 30-31 HYPH denotes -
T10945 31-34 JJ denotes old
T10947 35-39 NNS denotes mice
T10948 39-41 , denotes ,
T10949 41-49 VBN denotes perfused
T10950 50-54 IN denotes with
T10951 55-56 CD denotes 4
T10952 56-57 NN denotes %
T10953 58-74 NN denotes paraformaldehyde
T10954 75-77 IN denotes in
T10955 78-81 NN denotes PBS
T10956 81-83 , denotes ,
T10957 83-86 CC denotes and
T10958 87-97 VBN denotes post-fixed
T10959 98-107 RB denotes overnight
T10960 108-110 IN denotes in
T10961 111-114 DT denotes the
T10963 115-119 JJ denotes same
T10962 120-128 NN denotes fixative
T10964 128-129 . denotes .
T10965 129-291 sentence denotes Brains were embedded in gelatin, and 35-μm sagittal sections were cut using a sliding microtome (NeuroScience Associates, http://www.neuroscienceassociates.com).
T10966 130-136 NNS denotes Brains
T10968 137-141 VBD denotes were
T10967 142-150 VBN denotes embedded
T10969 151-153 IN denotes in
T10970 154-161 NN denotes gelatin
T10971 161-163 , denotes ,
T10972 163-166 CC denotes and
T10973 167-169 CD denotes 35
T10975 169-170 HYPH denotes -
T10974 170-172 NN denotes μm
T10977 173-181 JJ denotes sagittal
T10976 182-190 NNS denotes sections
T10979 191-195 VBD denotes were
T10978 196-199 VBN denotes cut
T10980 200-205 VBG denotes using
T10981 206-207 DT denotes a
T10983 208-215 VBG denotes sliding
T10982 216-225 NN denotes microtome
T10984 226-227 -LRB- denotes (
T10986 227-239 NNP denotes NeuroScience
T10985 240-250 NNP denotes Associates
T10987 250-252 , denotes ,
T10988 252-289 NN denotes http://www.neuroscienceassociates.com
T10989 289-290 -RRB- denotes )
T10990 290-291 . denotes .
T10991 291-392 sentence denotes Sections from wild-type, heterozygote, and homozygous brains were placed in the MultiBrain template.
T10992 292-300 NNS denotes Sections
T10994 301-305 IN denotes from
T10995 306-310 JJ denotes wild
T10997 310-311 HYPH denotes -
T10996 311-315 NN denotes type
T10999 315-317 , denotes ,
T11000 317-329 NN denotes heterozygote
T11001 329-331 , denotes ,
T11002 331-334 CC denotes and
T11003 335-345 JJ denotes homozygous
T10998 346-352 NNS denotes brains
T11004 353-357 VBD denotes were
T10993 358-364 VBN denotes placed
T11005 365-367 IN denotes in
T11006 368-371 DT denotes the
T11008 372-382 NN denotes MultiBrain
T11007 383-391 NN denotes template
T11009 391-392 . denotes .
T11010 392-545 sentence denotes Sections were washed in 1× PBS prior to 1 h of incubation in block solution containing 1× PBS with 20% normal goat serum and 0.3% Triton X-100 (pH 7.4).
T11011 393-401 NNS denotes Sections
T11013 402-406 VBD denotes were
T11012 407-413 VBN denotes washed
T11014 414-416 IN denotes in
T11015 417-418 CD denotes 1
T11017 418-419 SYM denotes ×
T11016 420-423 NN denotes PBS
T11018 424-429 RB denotes prior
T11019 430-432 IN denotes to
T11020 433-434 CD denotes 1
T11021 435-436 NN denotes h
T11022 437-439 IN denotes of
T11023 440-450 NN denotes incubation
T11024 451-453 IN denotes in
T11025 454-459 NN denotes block
T11026 460-468 NN denotes solution
T11027 469-479 VBG denotes containing
T11028 480-481 CD denotes 1
T11030 481-482 SYM denotes ×
T11029 483-486 NN denotes PBS
T11031 487-491 IN denotes with
T11032 492-494 CD denotes 20
T11033 494-495 NN denotes %
T11035 496-502 JJ denotes normal
T11036 503-507 NN denotes goat
T11034 508-513 NN denotes serum
T11037 514-517 CC denotes and
T11038 518-521 CD denotes 0.3
T11039 521-522 NN denotes %
T11041 523-529 NN denotes Triton
T11040 530-531 NN denotes X
T11042 531-532 HYPH denotes -
T11043 532-535 CD denotes 100
T11044 536-537 -LRB- denotes (
T11045 537-539 NN denotes pH
T11046 540-543 CD denotes 7.4
T11047 543-544 -RRB- denotes )
T11048 544-545 . denotes .
T11049 545-831 sentence denotes Sections were incubated overnight at 4 °C in primary antibodies: affinity purified polyclonal Itpr1 antibody (1:2,000, Chemicon International, http://www.chemicon.com) and monoclonal anti-Calb1 antibody (1:6,000, Sigma-Aldrich, http://www.sigmaaldrich.com) diluted in carrier solution.
T11050 546-554 NNS denotes Sections
T11052 555-559 VBD denotes were
T11051 560-569 VBN denotes incubated
T11053 570-579 RB denotes overnight
T11054 580-582 IN denotes at
T11055 583-584 CD denotes 4
T11056 585-587 NN denotes °C
T11057 588-590 IN denotes in
T11058 591-598 JJ denotes primary
T11059 599-609 NNS denotes antibodies
T11060 609-611 : denotes :
T11061 611-619 NN denotes affinity
T11063 620-628 VBN denotes purified
T11064 629-639 JJ denotes polyclonal
T11065 640-645 NN denotes Itpr1
T11062 646-654 NN denotes antibody
T11066 655-656 -LRB- denotes (
T11068 656-657 CD denotes 1
T11069 657-658 SYM denotes :
T11070 658-663 CD denotes 2,000
T11071 663-665 , denotes ,
T11072 665-673 NNP denotes Chemicon
T11067 674-687 NNP denotes International
T11073 687-689 , denotes ,
T11074 689-712 NN denotes http://www.chemicon.com
T11075 712-713 -RRB- denotes )
T11076 714-717 CC denotes and
T11077 718-728 JJ denotes monoclonal
T11079 729-739 JJ denotes anti-Calb1
T11078 740-748 NN denotes antibody
T11080 749-750 -LRB- denotes (
T11082 750-751 CD denotes 1
T11083 751-752 SYM denotes :
T11084 752-757 CD denotes 6,000
T11085 757-759 , denotes ,
T11086 759-764 NNP denotes Sigma
T11087 764-765 HYPH denotes -
T11081 765-772 NNP denotes Aldrich
T11088 772-774 , denotes ,
T11089 774-801 NN denotes http://www.sigmaaldrich.com
T11090 801-802 -RRB- denotes )
T11091 803-810 VBN denotes diluted
T11092 811-813 IN denotes in
T11093 814-821 NN denotes carrier
T11094 822-830 NN denotes solution
T11095 830-831 . denotes .
T11096 831-1101 sentence denotes Following extensive washes (in 6.0 ml of PBS, three times), sections were incubated with appropriate secondary antibodies (Alexa Fluor 555 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG [Invitrogen, http://www.invitrogen.com]) for 1 h at room temperature.
T11097 832-841 VBG denotes Following
T11099 842-851 JJ denotes extensive
T11100 852-858 NNS denotes washes
T11101 859-860 -LRB- denotes (
T11102 860-862 IN denotes in
T11103 863-866 CD denotes 6.0
T11104 867-869 NN denotes ml
T11105 870-872 IN denotes of
T11106 873-876 NN denotes PBS
T11107 876-878 , denotes ,
T11109 878-883 CD denotes three
T11108 884-889 NNS denotes times
T11110 889-890 -RRB- denotes )
T11111 890-892 , denotes ,
T11112 892-900 NNS denotes sections
T11113 901-905 VBD denotes were
T11098 906-915 VBN denotes incubated
T11114 916-920 IN denotes with
T11115 921-932 JJ denotes appropriate
T11117 933-942 JJ denotes secondary
T11116 943-953 NNS denotes antibodies
T11118 954-955 -LRB- denotes (
T11120 955-960 NNP denotes Alexa
T11121 961-966 NNP denotes Fluor
T11122 967-970 CD denotes 555
T11123 971-975 NN denotes goat
T11124 976-987 JJ denotes anti-rabbit
T11119 988-991 NN denotes IgG
T11125 992-995 CC denotes and
T11126 996-1001 NNP denotes Alexa
T11127 1002-1007 NNP denotes Fluor
T11129 1008-1011 CD denotes 488
T11130 1012-1016 NN denotes goat
T11131 1017-1027 JJ denotes anti-mouse
T11128 1028-1031 NN denotes IgG
T11132 1032-1033 -LRB- denotes [
T11133 1033-1043 NNP denotes Invitrogen
T11134 1043-1045 , denotes ,
T11135 1045-1070 NN denotes http://www.invitrogen.com
T11136 1070-1071 -RRB- denotes ]
T11137 1071-1072 -RRB- denotes )
T11138 1073-1076 IN denotes for
T11139 1077-1078 CD denotes 1
T11140 1079-1080 NN denotes h
T11141 1081-1083 IN denotes at
T11142 1084-1088 NN denotes room
T11143 1089-1100 NN denotes temperature
T11144 1100-1101 . denotes .
T11145 1101-1260 sentence denotes Sections were washed and mounted on glass slides in a buffered medium containing Mowiol (Calbiochem, http://www.emdbiosciences.com) as described earlier [14].
T11146 1102-1110 NNS denotes Sections
T11148 1111-1115 VBD denotes were
T11147 1116-1122 VBN denotes washed
T11149 1123-1126 CC denotes and
T11150 1127-1134 VBN denotes mounted
T11151 1135-1137 IN denotes on
T11152 1138-1143 NN denotes glass
T11153 1144-1150 NNS denotes slides
T11154 1151-1153 IN denotes in
T11155 1154-1155 DT denotes a
T11157 1156-1164 VBN denotes buffered
T11156 1165-1171 NN denotes medium
T11158 1172-1182 VBG denotes containing
T11159 1183-1189 NN denotes Mowiol
T11160 1190-1191 -LRB- denotes (
T11161 1191-1201 NNP denotes Calbiochem
T11162 1201-1203 , denotes ,
T11163 1203-1232 NN denotes http://www.emdbiosciences.com
T11164 1232-1233 -RRB- denotes )
T11165 1234-1236 IN denotes as
T11166 1237-1246 VBN denotes described
T11167 1247-1254 RBR denotes earlier
T11168 1255-1256 -LRB- denotes [
T11169 1256-1258 CD denotes 14
T11170 1258-1259 -RRB- denotes ]
T11171 1259-1260 . denotes .
T11172 1260-1364 sentence denotes Sections were imaged using a laser scanning confocal microscope (LSM 510; Zeiss, http://www.zeiss.com).
T11173 1261-1269 NNS denotes Sections
T11175 1270-1274 VBD denotes were
T11174 1275-1281 VBN denotes imaged
T11176 1282-1287 VBG denotes using
T11177 1288-1289 DT denotes a
T11179 1290-1295 NN denotes laser
T11180 1296-1304 VBG denotes scanning
T11181 1305-1313 JJ denotes confocal
T11178 1314-1324 NN denotes microscope
T11182 1325-1326 -LRB- denotes (
T11184 1326-1329 NN denotes LSM
T11185 1330-1333 CD denotes 510
T11186 1333-1334 : denotes ;
T11183 1335-1340 NNP denotes Zeiss
T11187 1340-1342 , denotes ,
T11188 1342-1362 NN denotes http://www.zeiss.com
T11189 1362-1363 -RRB- denotes )
T11190 1363-1364 . denotes .
T11191 1364-1523 sentence denotes Imaging parameters (pinhole, detector gain, laser power) were optimized, and were kept constant for the wild-type, heterozygous, and homozygous mutant brains.
T11192 1365-1372 NN denotes Imaging
T11193 1373-1383 NNS denotes parameters
T11195 1384-1385 -LRB- denotes (
T11196 1385-1392 NN denotes pinhole
T11197 1392-1394 , denotes ,
T11198 1394-1402 NN denotes detector
T11199 1403-1407 NN denotes gain
T11200 1407-1409 , denotes ,
T11201 1409-1414 NN denotes laser
T11202 1415-1420 NN denotes power
T11203 1420-1421 -RRB- denotes )
T11204 1422-1426 VBD denotes were
T11194 1427-1436 VBN denotes optimized
T11205 1436-1438 , denotes ,
T11206 1438-1441 CC denotes and
T11207 1442-1446 VBD denotes were
T11208 1447-1451 VBN denotes kept
T11209 1452-1460 JJ denotes constant
T11210 1461-1464 IN denotes for
T11211 1465-1468 DT denotes the
T11213 1469-1473 JJ denotes wild
T11215 1473-1474 HYPH denotes -
T11214 1474-1478 NN denotes type
T11216 1478-1480 , denotes ,
T11217 1480-1492 JJ denotes heterozygous
T11218 1492-1494 , denotes ,
T11219 1494-1497 CC denotes and
T11220 1498-1508 JJ denotes homozygous
T11221 1509-1515 NN denotes mutant
T11212 1516-1522 NNS denotes brains
T11222 1522-1523 . denotes .
T11223 1523-1610 sentence denotes Specificity of the Itpr1 antibodies was verified by preabsorption control experiments.
T11224 1524-1535 NN denotes Specificity
T11226 1536-1538 IN denotes of
T11227 1539-1542 DT denotes the
T11229 1543-1548 NN denotes Itpr1
T11228 1549-1559 NNS denotes antibodies
T11230 1560-1563 VBD denotes was
T11225 1564-1572 VBN denotes verified
T11231 1573-1575 IN denotes by
T11232 1576-1589 NN denotes preabsorption
T11234 1590-1597 NN denotes control
T11233 1598-1609 NNS denotes experiments
T11235 1609-1610 . denotes .
T11236 1610-1690 sentence denotes Antibody dilutions were incubated for 24 h at 4 °C with the immunizing peptide.
T11237 1611-1619 NN denotes Antibody
T11238 1620-1629 NNS denotes dilutions
T11240 1630-1634 VBD denotes were
T11239 1635-1644 VBN denotes incubated
T11241 1645-1648 IN denotes for
T11242 1649-1651 CD denotes 24
T11243 1652-1653 NN denotes h
T11244 1654-1656 IN denotes at
T11245 1657-1658 CD denotes 4
T11246 1659-1661 NN denotes °C
T11247 1662-1666 IN denotes with
T11248 1667-1670 DT denotes the
T11250 1671-1681 VBG denotes immunizing
T11249 1682-1689 NN denotes peptide
T11251 1689-1690 . denotes .
T11252 1690-1787 sentence denotes Tissue sections were incubated with the preabsorbed antibodies and processed as described above.
T11253 1691-1697 NN denotes Tissue
T11254 1698-1706 NNS denotes sections
T11256 1707-1711 VBD denotes were
T11255 1712-1721 VBN denotes incubated
T11257 1722-1726 IN denotes with
T11258 1727-1730 DT denotes the
T11260 1731-1742 VBN denotes preabsorbed
T11259 1743-1753 NNS denotes antibodies
T11261 1754-1757 CC denotes and
T11262 1758-1767 VBN denotes processed
T11263 1768-1770 IN denotes as
T11264 1771-1780 VBN denotes described
T11265 1781-1786 RB denotes above
T11266 1786-1787 . denotes .
T11267 1787-1860 sentence denotes Under these conditions, no staining above autofluorescence was detected.
T11268 1788-1793 IN denotes Under
T11270 1794-1799 DT denotes these
T11271 1800-1810 NNS denotes conditions
T11272 1810-1812 , denotes ,
T11273 1812-1814 DT denotes no
T11274 1815-1823 NN denotes staining
T11275 1824-1829 IN denotes above
T11276 1830-1846 NN denotes autofluorescence
T11277 1847-1850 VBD denotes was
T11269 1851-1859 VBN denotes detected
T11278 1859-1860 . denotes .
R3037 T10938 T10939 nsubjpass Brains,isolated
R3038 T10940 T10939 auxpass were,isolated
R3039 T10941 T10939 prep from,isolated
R3040 T10942 T10943 nummod 21,d
R3041 T10943 T10945 npadvmod d,old
R3042 T10944 T10943 punct -,d
R3043 T10945 T10947 amod old,mice
R3044 T10946 T10945 punct -,old
R3045 T10947 T10941 pobj mice,from
R3046 T10948 T10939 punct ", ",isolated
R3047 T10949 T10939 conj perfused,isolated
R3048 T10950 T10949 prep with,perfused
R3049 T10951 T10952 nummod 4,%
R3050 T10952 T10953 compound %,paraformaldehyde
R3051 T10953 T10950 pobj paraformaldehyde,with
R3052 T10954 T10953 prep in,paraformaldehyde
R3053 T10955 T10954 pobj PBS,in
R3054 T10956 T10949 punct ", ",perfused
R3055 T10957 T10949 cc and,perfused
R3056 T10958 T10949 conj post-fixed,perfused
R3057 T10959 T10958 advmod overnight,post-fixed
R3058 T10960 T10958 prep in,post-fixed
R3059 T10961 T10962 det the,fixative
R3060 T10962 T10960 pobj fixative,in
R3061 T10963 T10962 amod same,fixative
R3062 T10964 T10939 punct .,isolated
R3063 T10966 T10967 nsubjpass Brains,embedded
R3064 T10968 T10967 auxpass were,embedded
R3065 T10969 T10967 prep in,embedded
R3066 T10970 T10969 pobj gelatin,in
R3067 T10971 T10967 punct ", ",embedded
R3068 T10972 T10967 cc and,embedded
R3069 T10973 T10974 nummod 35,μm
R3070 T10974 T10976 nmod μm,sections
R3071 T10975 T10974 punct -,μm
R3072 T10976 T10978 nsubjpass sections,cut
R3073 T10977 T10976 amod sagittal,sections
R3074 T10978 T10967 conj cut,embedded
R3075 T10979 T10978 auxpass were,cut
R3076 T10980 T10978 advcl using,cut
R3077 T10981 T10982 det a,microtome
R3078 T10982 T10980 dobj microtome,using
R3079 T10983 T10982 amod sliding,microtome
R3080 T10984 T10985 punct (,Associates
R3081 T10985 T10982 parataxis Associates,microtome
R3082 T10986 T10985 compound NeuroScience,Associates
R3083 T10987 T10985 punct ", ",Associates
R3084 T10988 T10985 npadvmod http://www.neuroscienceassociates.com,Associates
R3085 T10989 T10985 punct ),Associates
R3086 T10990 T10978 punct .,cut
R3087 T10992 T10993 nsubjpass Sections,placed
R3088 T10994 T10992 prep from,Sections
R3089 T10995 T10996 amod wild,type
R3090 T10996 T10998 nmod type,brains
R3091 T10997 T10996 punct -,type
R3092 T10998 T10994 pobj brains,from
R3093 T10999 T10996 punct ", ",type
R3094 T11000 T10996 conj heterozygote,type
R3095 T11001 T11000 punct ", ",heterozygote
R3096 T11002 T11000 cc and,heterozygote
R3097 T11003 T11000 conj homozygous,heterozygote
R3098 T11004 T10993 auxpass were,placed
R3099 T11005 T10993 prep in,placed
R3100 T11006 T11007 det the,template
R3101 T11007 T11005 pobj template,in
R3102 T11008 T11007 compound MultiBrain,template
R3103 T11009 T10993 punct .,placed
R3104 T11011 T11012 nsubjpass Sections,washed
R3105 T11013 T11012 auxpass were,washed
R3106 T11014 T11012 prep in,washed
R3107 T11015 T11016 nummod 1,PBS
R3108 T11016 T11014 pobj PBS,in
R3109 T11017 T11015 punct ×,1
R3110 T11018 T11019 advmod prior,to
R3111 T11019 T11012 prep to,washed
R3112 T11020 T11021 nummod 1,h
R3113 T11021 T11019 pobj h,to
R3114 T11022 T11021 prep of,h
R3115 T11023 T11022 pobj incubation,of
R3116 T11024 T11023 prep in,incubation
R3117 T11025 T11026 compound block,solution
R3118 T11026 T11024 pobj solution,in
R3119 T11027 T11026 acl containing,solution
R3120 T11028 T11029 nummod 1,PBS
R3121 T11029 T11027 dobj PBS,containing
R3122 T11030 T11028 punct ×,1
R3123 T11031 T11029 prep with,PBS
R3124 T11032 T11033 nummod 20,%
R3125 T11033 T11034 nmod %,serum
R3126 T11034 T11031 pobj serum,with
R3127 T11035 T11034 amod normal,serum
R3128 T11036 T11034 compound goat,serum
R3129 T11037 T11034 cc and,serum
R3130 T11038 T11039 nummod 0.3,%
R3131 T11039 T11040 compound %,X
R3132 T11040 T11034 conj X,serum
R3133 T11041 T11040 compound Triton,X
R3134 T11042 T11040 punct -,X
R3135 T11043 T11040 nummod 100,X
R3136 T11044 T11045 punct (,pH
R3137 T11045 T11040 parataxis pH,X
R3138 T11046 T11045 nummod 7.4,pH
R3139 T11047 T11045 punct ),pH
R3140 T11048 T11012 punct .,washed
R3141 T11050 T11051 nsubjpass Sections,incubated
R3142 T11052 T11051 auxpass were,incubated
R3143 T11053 T11051 advmod overnight,incubated
R3144 T11054 T11051 prep at,incubated
R3145 T11055 T11056 nummod 4,°C
R3146 T11056 T11054 pobj °C,at
R3147 T11057 T11051 prep in,incubated
R3148 T11058 T11059 amod primary,antibodies
R3149 T11059 T11057 pobj antibodies,in
R3150 T11060 T11059 punct : ,antibodies
R3151 T11061 T11062 nmod affinity,antibody
R3152 T11062 T11059 appos antibody,antibodies
R3153 T11063 T11062 amod purified,antibody
R3154 T11064 T11062 amod polyclonal,antibody
R3155 T11065 T11062 compound Itpr1,antibody
R3156 T11066 T11067 punct (,International
R3157 T11067 T11062 parataxis International,antibody
R3158 T11068 T11067 dep 1,International
R3159 T11069 T11070 punct :,"2,000"
R3160 T11070 T11068 prep "2,000",1
R3161 T11071 T11067 punct ", ",International
R3162 T11072 T11067 compound Chemicon,International
R3163 T11073 T11067 punct ", ",International
R3164 T11074 T11067 npadvmod http://www.chemicon.com,International
R3165 T11075 T11067 punct ),International
R3166 T11076 T11062 cc and,antibody
R3167 T11077 T11078 amod monoclonal,antibody
R3168 T11078 T11062 conj antibody,antibody
R3169 T11079 T11078 amod anti-Calb1,antibody
R3170 T11080 T11081 punct (,Aldrich
R3171 T11081 T11078 parataxis Aldrich,antibody
R3172 T11082 T11081 dep 1,Aldrich
R3173 T11083 T11084 punct :,"6,000"
R3174 T11084 T11082 prep "6,000",1
R3175 T11085 T11081 punct ", ",Aldrich
R3176 T11086 T11081 compound Sigma,Aldrich
R3177 T11087 T11081 punct -,Aldrich
R3178 T11088 T11081 punct ", ",Aldrich
R3179 T11089 T11081 npadvmod http://www.sigmaaldrich.com,Aldrich
R3180 T11090 T11081 punct ),Aldrich
R3181 T11091 T11062 acl diluted,antibody
R3182 T11092 T11091 prep in,diluted
R3183 T11093 T11094 compound carrier,solution
R3184 T11094 T11092 pobj solution,in
R3185 T11095 T11051 punct .,incubated
R3186 T11097 T11098 prep Following,incubated
R3187 T11099 T11100 amod extensive,washes
R3188 T11100 T11097 pobj washes,Following
R3189 T11101 T11100 punct (,washes
R3190 T11102 T11100 prep in,washes
R3191 T11103 T11104 nummod 6.0,ml
R3192 T11104 T11102 pobj ml,in
R3193 T11105 T11104 prep of,ml
R3194 T11106 T11105 pobj PBS,of
R3195 T11107 T11108 punct ", ",times
R3196 T11108 T11100 parataxis times,washes
R3197 T11109 T11108 nummod three,times
R3198 T11110 T11108 punct ),times
R3199 T11111 T11098 punct ", ",incubated
R3200 T11112 T11098 nsubjpass sections,incubated
R3201 T11113 T11098 auxpass were,incubated
R3202 T11114 T11098 prep with,incubated
R3203 T11115 T11116 amod appropriate,antibodies
R3204 T11116 T11114 pobj antibodies,with
R3205 T11117 T11116 amod secondary,antibodies
R3206 T11118 T11119 punct (,IgG
R3207 T11119 T11116 parataxis IgG,antibodies
R3208 T11120 T11121 nmod Alexa,Fluor
R3209 T11121 T11119 nmod Fluor,IgG
R3210 T11122 T11121 nummod 555,Fluor
R3211 T11123 T11119 nmod goat,IgG
R3212 T11124 T11119 amod anti-rabbit,IgG
R3213 T11125 T11119 cc and,IgG
R3214 T11126 T11127 nmod Alexa,Fluor
R3215 T11127 T11128 nmod Fluor,IgG
R3216 T11128 T11119 conj IgG,IgG
R3217 T11129 T11127 nummod 488,Fluor
R3218 T11130 T11128 nmod goat,IgG
R3219 T11131 T11128 amod anti-mouse,IgG
R3220 T11132 T11133 punct [,Invitrogen
R3221 T11133 T11128 parataxis Invitrogen,IgG
R3222 T11134 T11133 punct ", ",Invitrogen
R3223 T11135 T11133 npadvmod http://www.invitrogen.com,Invitrogen
R3224 T11136 T11133 punct ],Invitrogen
R3225 T11137 T11119 punct ),IgG
R3226 T11138 T11098 prep for,incubated
R3227 T11139 T11140 nummod 1,h
R3228 T11140 T11138 pobj h,for
R3229 T11141 T11098 prep at,incubated
R3230 T11142 T11143 compound room,temperature
R3231 T11143 T11141 pobj temperature,at
R3232 T11144 T11098 punct .,incubated
R3233 T11146 T11147 nsubjpass Sections,washed
R3234 T11148 T11147 auxpass were,washed
R3235 T11149 T11147 cc and,washed
R3236 T11150 T11147 conj mounted,washed
R3237 T11151 T11150 prep on,mounted
R3238 T11152 T11153 compound glass,slides
R3239 T11153 T11151 pobj slides,on
R3240 T11154 T11150 prep in,mounted
R3241 T11155 T11156 det a,medium
R3242 T11156 T11154 pobj medium,in
R3243 T11157 T11156 amod buffered,medium
R3244 T11158 T11156 acl containing,medium
R3245 T11159 T11158 dobj Mowiol,containing
R3246 T11160 T11161 punct (,Calbiochem
R3247 T11161 T11159 parataxis Calbiochem,Mowiol
R3248 T11162 T11161 punct ", ",Calbiochem
R3249 T11163 T11161 npadvmod http://www.emdbiosciences.com,Calbiochem
R3250 T11164 T11161 punct ),Calbiochem
R3251 T11165 T11166 mark as,described
R3252 T11166 T11150 advcl described,mounted
R3253 T11167 T11166 advmod earlier,described
R3254 T11168 T11169 punct [,14
R3255 T11169 T11150 parataxis 14,mounted
R3256 T11170 T11169 punct ],14
R3257 T11171 T11147 punct .,washed
R3258 T11173 T11174 nsubjpass Sections,imaged
R3259 T11175 T11174 auxpass were,imaged
R3260 T11176 T11174 advcl using,imaged
R3261 T11177 T11178 det a,microscope
R3262 T11178 T11176 dobj microscope,using
R3263 T11179 T11178 nmod laser,microscope
R3264 T11180 T11178 amod scanning,microscope
R3265 T11181 T11178 amod confocal,microscope
R3266 T11182 T11183 punct (,Zeiss
R3267 T11183 T11178 parataxis Zeiss,microscope
R3268 T11184 T11183 dep LSM,Zeiss
R3269 T11185 T11184 nummod 510,LSM
R3270 T11186 T11183 punct ;,Zeiss
R3271 T11187 T11183 punct ", ",Zeiss
R3272 T11188 T11183 npadvmod http://www.zeiss.com,Zeiss
R3273 T11189 T11183 punct ),Zeiss
R3274 T11190 T11174 punct .,imaged
R3275 T11192 T11193 compound Imaging,parameters
R3276 T11193 T11194 nsubjpass parameters,optimized
R3277 T11195 T11196 punct (,pinhole
R3278 T11196 T11193 parataxis pinhole,parameters
R3279 T11197 T11196 punct ", ",pinhole
R3280 T11198 T11199 compound detector,gain
R3281 T11199 T11196 appos gain,pinhole
R3282 T11200 T11196 punct ", ",pinhole
R3283 T11201 T11202 compound laser,power
R3284 T11202 T11196 appos power,pinhole
R3285 T11203 T11196 punct ),pinhole
R3286 T11204 T11194 auxpass were,optimized
R3287 T11205 T11194 punct ", ",optimized
R3288 T11206 T11194 cc and,optimized
R3289 T11207 T11208 auxpass were,kept
R3290 T11208 T11194 conj kept,optimized
R3291 T11209 T11208 oprd constant,kept
R3292 T11210 T11208 prep for,kept
R3293 T11211 T11212 det the,brains
R3294 T11212 T11210 pobj brains,for
R3295 T11213 T11214 amod wild,type
R3296 T11214 T11212 nmod type,brains
R3297 T11215 T11214 punct -,type
R3298 T11216 T11214 punct ", ",type
R3299 T11217 T11214 conj heterozygous,type
R3300 T11218 T11217 punct ", ",heterozygous
R3301 T11219 T11217 cc and,heterozygous
R3302 T11220 T11217 conj homozygous,heterozygous
R3303 T11221 T11212 compound mutant,brains
R3304 T11222 T11194 punct .,optimized
R3305 T11224 T11225 nsubjpass Specificity,verified
R3306 T11226 T11224 prep of,Specificity
R3307 T11227 T11228 det the,antibodies
R3308 T11228 T11226 pobj antibodies,of
R3309 T11229 T11228 compound Itpr1,antibodies
R3310 T11230 T11225 auxpass was,verified
R3311 T11231 T11225 prep by,verified
R3312 T11232 T11233 compound preabsorption,experiments
R3313 T11233 T11231 pobj experiments,by
R3314 T11234 T11233 compound control,experiments
R3315 T11235 T11225 punct .,verified
R3316 T11237 T11238 compound Antibody,dilutions
R3317 T11238 T11239 nsubjpass dilutions,incubated
R3318 T11240 T11239 auxpass were,incubated
R3319 T11241 T11239 prep for,incubated
R3320 T11242 T11243 nummod 24,h
R3321 T11243 T11241 pobj h,for
R3322 T11244 T11239 prep at,incubated
R3323 T11245 T11246 nummod 4,°C
R3324 T11246 T11244 pobj °C,at
R3325 T11247 T11239 prep with,incubated
R3326 T11248 T11249 det the,peptide
R3327 T11249 T11247 pobj peptide,with
R3328 T11250 T11249 amod immunizing,peptide
R3329 T11251 T11239 punct .,incubated
R3330 T11253 T11254 compound Tissue,sections
R3331 T11254 T11255 nsubjpass sections,incubated
R3332 T11256 T11255 auxpass were,incubated
R3333 T11257 T11255 prep with,incubated
R3334 T11258 T11259 det the,antibodies
R3335 T11259 T11257 pobj antibodies,with
R3336 T11260 T11259 amod preabsorbed,antibodies
R3337 T11261 T11255 cc and,incubated
R3338 T11262 T11255 conj processed,incubated
R3339 T11263 T11264 mark as,described
R3340 T11264 T11262 advcl described,processed
R3341 T11265 T11264 advmod above,described
R3342 T11266 T11255 punct .,incubated
R3343 T11268 T11269 prep Under,detected
R3344 T11270 T11271 det these,conditions
R3345 T11271 T11268 pobj conditions,Under
R3346 T11272 T11269 punct ", ",detected
R3347 T11273 T11274 det no,staining
R3348 T11274 T11269 nsubjpass staining,detected
R3349 T11275 T11274 prep above,staining
R3350 T11276 T11275 pobj autofluorescence,above
R3351 T11277 T11269 auxpass was,detected
R3352 T11278 T11269 punct .,detected