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PMC:1892049 / 21947-23829 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T10935 0-20 NN denotes Immunohistochemistry
T10936 20-21 . denotes .
T10937 21-151 sentence denotes Brains were isolated from 21-d-old mice, perfused with 4% paraformaldehyde in PBS, and post-fixed overnight in the same fixative.
T10938 22-28 NNS denotes Brains
T10940 29-33 VBD denotes were
T10939 34-42 VBN denotes isolated
T10941 43-47 IN denotes from
T10942 48-50 CD denotes 21
T10944 50-51 HYPH denotes -
T10943 51-52 NN denotes d
T10946 52-53 HYPH denotes -
T10945 53-56 JJ denotes old
T10947 57-61 NNS denotes mice
T10948 61-63 , denotes ,
T10949 63-71 VBN denotes perfused
T10950 72-76 IN denotes with
T10951 77-78 CD denotes 4
T10952 78-79 NN denotes %
T10953 80-96 NN denotes paraformaldehyde
T10954 97-99 IN denotes in
T10955 100-103 NN denotes PBS
T10956 103-105 , denotes ,
T10957 105-108 CC denotes and
T10958 109-119 VBN denotes post-fixed
T10959 120-129 RB denotes overnight
T10960 130-132 IN denotes in
T10961 133-136 DT denotes the
T10963 137-141 JJ denotes same
T10962 142-150 NN denotes fixative
T10964 150-151 . denotes .
T10965 151-313 sentence denotes Brains were embedded in gelatin, and 35-μm sagittal sections were cut using a sliding microtome (NeuroScience Associates, http://www.neuroscienceassociates.com).
T10966 152-158 NNS denotes Brains
T10968 159-163 VBD denotes were
T10967 164-172 VBN denotes embedded
T10969 173-175 IN denotes in
T10970 176-183 NN denotes gelatin
T10971 183-185 , denotes ,
T10972 185-188 CC denotes and
T10973 189-191 CD denotes 35
T10975 191-192 HYPH denotes -
T10974 192-194 NN denotes μm
T10977 195-203 JJ denotes sagittal
T10976 204-212 NNS denotes sections
T10979 213-217 VBD denotes were
T10978 218-221 VBN denotes cut
T10980 222-227 VBG denotes using
T10981 228-229 DT denotes a
T10983 230-237 VBG denotes sliding
T10982 238-247 NN denotes microtome
T10984 248-249 -LRB- denotes (
T10986 249-261 NNP denotes NeuroScience
T10985 262-272 NNP denotes Associates
T10987 272-274 , denotes ,
T10988 274-311 NN denotes http://www.neuroscienceassociates.com
T10989 311-312 -RRB- denotes )
T10990 312-313 . denotes .
T10991 313-414 sentence denotes Sections from wild-type, heterozygote, and homozygous brains were placed in the MultiBrain template.
T10992 314-322 NNS denotes Sections
T10994 323-327 IN denotes from
T10995 328-332 JJ denotes wild
T10997 332-333 HYPH denotes -
T10996 333-337 NN denotes type
T10999 337-339 , denotes ,
T11000 339-351 NN denotes heterozygote
T11001 351-353 , denotes ,
T11002 353-356 CC denotes and
T11003 357-367 JJ denotes homozygous
T10998 368-374 NNS denotes brains
T11004 375-379 VBD denotes were
T10993 380-386 VBN denotes placed
T11005 387-389 IN denotes in
T11006 390-393 DT denotes the
T11008 394-404 NN denotes MultiBrain
T11007 405-413 NN denotes template
T11009 413-414 . denotes .
T11010 414-567 sentence denotes Sections were washed in 1× PBS prior to 1 h of incubation in block solution containing 1× PBS with 20% normal goat serum and 0.3% Triton X-100 (pH 7.4).
T11011 415-423 NNS denotes Sections
T11013 424-428 VBD denotes were
T11012 429-435 VBN denotes washed
T11014 436-438 IN denotes in
T11015 439-440 CD denotes 1
T11017 440-441 SYM denotes ×
T11016 442-445 NN denotes PBS
T11018 446-451 RB denotes prior
T11019 452-454 IN denotes to
T11020 455-456 CD denotes 1
T11021 457-458 NN denotes h
T11022 459-461 IN denotes of
T11023 462-472 NN denotes incubation
T11024 473-475 IN denotes in
T11025 476-481 NN denotes block
T11026 482-490 NN denotes solution
T11027 491-501 VBG denotes containing
T11028 502-503 CD denotes 1
T11030 503-504 SYM denotes ×
T11029 505-508 NN denotes PBS
T11031 509-513 IN denotes with
T11032 514-516 CD denotes 20
T11033 516-517 NN denotes %
T11035 518-524 JJ denotes normal
T11036 525-529 NN denotes goat
T11034 530-535 NN denotes serum
T11037 536-539 CC denotes and
T11038 540-543 CD denotes 0.3
T11039 543-544 NN denotes %
T11041 545-551 NN denotes Triton
T11040 552-553 NN denotes X
T11042 553-554 HYPH denotes -
T11043 554-557 CD denotes 100
T11044 558-559 -LRB- denotes (
T11045 559-561 NN denotes pH
T11046 562-565 CD denotes 7.4
T11047 565-566 -RRB- denotes )
T11048 566-567 . denotes .
T11049 567-853 sentence denotes Sections were incubated overnight at 4 °C in primary antibodies: affinity purified polyclonal Itpr1 antibody (1:2,000, Chemicon International, http://www.chemicon.com) and monoclonal anti-Calb1 antibody (1:6,000, Sigma-Aldrich, http://www.sigmaaldrich.com) diluted in carrier solution.
T11050 568-576 NNS denotes Sections
T11052 577-581 VBD denotes were
T11051 582-591 VBN denotes incubated
T11053 592-601 RB denotes overnight
T11054 602-604 IN denotes at
T11055 605-606 CD denotes 4
T11056 607-609 NN denotes °C
T11057 610-612 IN denotes in
T11058 613-620 JJ denotes primary
T11059 621-631 NNS denotes antibodies
T11060 631-633 : denotes :
T11061 633-641 NN denotes affinity
T11063 642-650 VBN denotes purified
T11064 651-661 JJ denotes polyclonal
T11065 662-667 NN denotes Itpr1
T11062 668-676 NN denotes antibody
T11066 677-678 -LRB- denotes (
T11068 678-679 CD denotes 1
T11069 679-680 SYM denotes :
T11070 680-685 CD denotes 2,000
T11071 685-687 , denotes ,
T11072 687-695 NNP denotes Chemicon
T11067 696-709 NNP denotes International
T11073 709-711 , denotes ,
T11074 711-734 NN denotes http://www.chemicon.com
T11075 734-735 -RRB- denotes )
T11076 736-739 CC denotes and
T11077 740-750 JJ denotes monoclonal
T11079 751-761 JJ denotes anti-Calb1
T11078 762-770 NN denotes antibody
T11080 771-772 -LRB- denotes (
T11082 772-773 CD denotes 1
T11083 773-774 SYM denotes :
T11084 774-779 CD denotes 6,000
T11085 779-781 , denotes ,
T11086 781-786 NNP denotes Sigma
T11087 786-787 HYPH denotes -
T11081 787-794 NNP denotes Aldrich
T11088 794-796 , denotes ,
T11089 796-823 NN denotes http://www.sigmaaldrich.com
T11090 823-824 -RRB- denotes )
T11091 825-832 VBN denotes diluted
T11092 833-835 IN denotes in
T11093 836-843 NN denotes carrier
T11094 844-852 NN denotes solution
T11095 852-853 . denotes .
T11096 853-1123 sentence denotes Following extensive washes (in 6.0 ml of PBS, three times), sections were incubated with appropriate secondary antibodies (Alexa Fluor 555 goat anti-rabbit IgG and Alexa Fluor 488 goat anti-mouse IgG [Invitrogen, http://www.invitrogen.com]) for 1 h at room temperature.
T11097 854-863 VBG denotes Following
T11099 864-873 JJ denotes extensive
T11100 874-880 NNS denotes washes
T11101 881-882 -LRB- denotes (
T11102 882-884 IN denotes in
T11103 885-888 CD denotes 6.0
T11104 889-891 NN denotes ml
T11105 892-894 IN denotes of
T11106 895-898 NN denotes PBS
T11107 898-900 , denotes ,
T11109 900-905 CD denotes three
T11108 906-911 NNS denotes times
T11110 911-912 -RRB- denotes )
T11111 912-914 , denotes ,
T11112 914-922 NNS denotes sections
T11113 923-927 VBD denotes were
T11098 928-937 VBN denotes incubated
T11114 938-942 IN denotes with
T11115 943-954 JJ denotes appropriate
T11117 955-964 JJ denotes secondary
T11116 965-975 NNS denotes antibodies
T11118 976-977 -LRB- denotes (
T11120 977-982 NNP denotes Alexa
T11121 983-988 NNP denotes Fluor
T11122 989-992 CD denotes 555
T11123 993-997 NN denotes goat
T11124 998-1009 JJ denotes anti-rabbit
T11119 1010-1013 NN denotes IgG
T11125 1014-1017 CC denotes and
T11126 1018-1023 NNP denotes Alexa
T11127 1024-1029 NNP denotes Fluor
T11129 1030-1033 CD denotes 488
T11130 1034-1038 NN denotes goat
T11131 1039-1049 JJ denotes anti-mouse
T11128 1050-1053 NN denotes IgG
T11132 1054-1055 -LRB- denotes [
T11133 1055-1065 NNP denotes Invitrogen
T11134 1065-1067 , denotes ,
T11135 1067-1092 NN denotes http://www.invitrogen.com
T11136 1092-1093 -RRB- denotes ]
T11137 1093-1094 -RRB- denotes )
T11138 1095-1098 IN denotes for
T11139 1099-1100 CD denotes 1
T11140 1101-1102 NN denotes h
T11141 1103-1105 IN denotes at
T11142 1106-1110 NN denotes room
T11143 1111-1122 NN denotes temperature
T11144 1122-1123 . denotes .
T11145 1123-1282 sentence denotes Sections were washed and mounted on glass slides in a buffered medium containing Mowiol (Calbiochem, http://www.emdbiosciences.com) as described earlier [14].
T11146 1124-1132 NNS denotes Sections
T11148 1133-1137 VBD denotes were
T11147 1138-1144 VBN denotes washed
T11149 1145-1148 CC denotes and
T11150 1149-1156 VBN denotes mounted
T11151 1157-1159 IN denotes on
T11152 1160-1165 NN denotes glass
T11153 1166-1172 NNS denotes slides
T11154 1173-1175 IN denotes in
T11155 1176-1177 DT denotes a
T11157 1178-1186 VBN denotes buffered
T11156 1187-1193 NN denotes medium
T11158 1194-1204 VBG denotes containing
T11159 1205-1211 NN denotes Mowiol
T11160 1212-1213 -LRB- denotes (
T11161 1213-1223 NNP denotes Calbiochem
T11162 1223-1225 , denotes ,
T11163 1225-1254 NN denotes http://www.emdbiosciences.com
T11164 1254-1255 -RRB- denotes )
T11165 1256-1258 IN denotes as
T11166 1259-1268 VBN denotes described
T11167 1269-1276 RBR denotes earlier
T11168 1277-1278 -LRB- denotes [
T11169 1278-1280 CD denotes 14
T11170 1280-1281 -RRB- denotes ]
T11171 1281-1282 . denotes .
T11172 1282-1386 sentence denotes Sections were imaged using a laser scanning confocal microscope (LSM 510; Zeiss, http://www.zeiss.com).
T11173 1283-1291 NNS denotes Sections
T11175 1292-1296 VBD denotes were
T11174 1297-1303 VBN denotes imaged
T11176 1304-1309 VBG denotes using
T11177 1310-1311 DT denotes a
T11179 1312-1317 NN denotes laser
T11180 1318-1326 VBG denotes scanning
T11181 1327-1335 JJ denotes confocal
T11178 1336-1346 NN denotes microscope
T11182 1347-1348 -LRB- denotes (
T11184 1348-1351 NN denotes LSM
T11185 1352-1355 CD denotes 510
T11186 1355-1356 : denotes ;
T11183 1357-1362 NNP denotes Zeiss
T11187 1362-1364 , denotes ,
T11188 1364-1384 NN denotes http://www.zeiss.com
T11189 1384-1385 -RRB- denotes )
T11190 1385-1386 . denotes .
T11191 1386-1545 sentence denotes Imaging parameters (pinhole, detector gain, laser power) were optimized, and were kept constant for the wild-type, heterozygous, and homozygous mutant brains.
T11192 1387-1394 NN denotes Imaging
T11193 1395-1405 NNS denotes parameters
T11195 1406-1407 -LRB- denotes (
T11196 1407-1414 NN denotes pinhole
T11197 1414-1416 , denotes ,
T11198 1416-1424 NN denotes detector
T11199 1425-1429 NN denotes gain
T11200 1429-1431 , denotes ,
T11201 1431-1436 NN denotes laser
T11202 1437-1442 NN denotes power
T11203 1442-1443 -RRB- denotes )
T11204 1444-1448 VBD denotes were
T11194 1449-1458 VBN denotes optimized
T11205 1458-1460 , denotes ,
T11206 1460-1463 CC denotes and
T11207 1464-1468 VBD denotes were
T11208 1469-1473 VBN denotes kept
T11209 1474-1482 JJ denotes constant
T11210 1483-1486 IN denotes for
T11211 1487-1490 DT denotes the
T11213 1491-1495 JJ denotes wild
T11215 1495-1496 HYPH denotes -
T11214 1496-1500 NN denotes type
T11216 1500-1502 , denotes ,
T11217 1502-1514 JJ denotes heterozygous
T11218 1514-1516 , denotes ,
T11219 1516-1519 CC denotes and
T11220 1520-1530 JJ denotes homozygous
T11221 1531-1537 NN denotes mutant
T11212 1538-1544 NNS denotes brains
T11222 1544-1545 . denotes .
T11223 1545-1632 sentence denotes Specificity of the Itpr1 antibodies was verified by preabsorption control experiments.
T11224 1546-1557 NN denotes Specificity
T11226 1558-1560 IN denotes of
T11227 1561-1564 DT denotes the
T11229 1565-1570 NN denotes Itpr1
T11228 1571-1581 NNS denotes antibodies
T11230 1582-1585 VBD denotes was
T11225 1586-1594 VBN denotes verified
T11231 1595-1597 IN denotes by
T11232 1598-1611 NN denotes preabsorption
T11234 1612-1619 NN denotes control
T11233 1620-1631 NNS denotes experiments
T11235 1631-1632 . denotes .
T11236 1632-1712 sentence denotes Antibody dilutions were incubated for 24 h at 4 °C with the immunizing peptide.
T11237 1633-1641 NN denotes Antibody
T11238 1642-1651 NNS denotes dilutions
T11240 1652-1656 VBD denotes were
T11239 1657-1666 VBN denotes incubated
T11241 1667-1670 IN denotes for
T11242 1671-1673 CD denotes 24
T11243 1674-1675 NN denotes h
T11244 1676-1678 IN denotes at
T11245 1679-1680 CD denotes 4
T11246 1681-1683 NN denotes °C
T11247 1684-1688 IN denotes with
T11248 1689-1692 DT denotes the
T11250 1693-1703 VBG denotes immunizing
T11249 1704-1711 NN denotes peptide
T11251 1711-1712 . denotes .
T11252 1712-1809 sentence denotes Tissue sections were incubated with the preabsorbed antibodies and processed as described above.
T11253 1713-1719 NN denotes Tissue
T11254 1720-1728 NNS denotes sections
T11256 1729-1733 VBD denotes were
T11255 1734-1743 VBN denotes incubated
T11257 1744-1748 IN denotes with
T11258 1749-1752 DT denotes the
T11260 1753-1764 VBN denotes preabsorbed
T11259 1765-1775 NNS denotes antibodies
T11261 1776-1779 CC denotes and
T11262 1780-1789 VBN denotes processed
T11263 1790-1792 IN denotes as
T11264 1793-1802 VBN denotes described
T11265 1803-1808 RB denotes above
T11266 1808-1809 . denotes .
T11267 1809-1882 sentence denotes Under these conditions, no staining above autofluorescence was detected.
T11268 1810-1815 IN denotes Under
T11270 1816-1821 DT denotes these
T11271 1822-1832 NNS denotes conditions
T11272 1832-1834 , denotes ,
T11273 1834-1836 DT denotes no
T11274 1837-1845 NN denotes staining
T11275 1846-1851 IN denotes above
T11276 1852-1868 NN denotes autofluorescence
T11277 1869-1872 VBD denotes was
T11269 1873-1881 VBN denotes detected
T11278 1881-1882 . denotes .
R3036 T10936 T10935 punct .,Immunohistochemistry
R3037 T10938 T10939 nsubjpass Brains,isolated
R3038 T10940 T10939 auxpass were,isolated
R3039 T10941 T10939 prep from,isolated
R3040 T10942 T10943 nummod 21,d
R3041 T10943 T10945 npadvmod d,old
R3042 T10944 T10943 punct -,d
R3043 T10945 T10947 amod old,mice
R3044 T10946 T10945 punct -,old
R3045 T10947 T10941 pobj mice,from
R3046 T10948 T10939 punct ", ",isolated
R3047 T10949 T10939 conj perfused,isolated
R3048 T10950 T10949 prep with,perfused
R3049 T10951 T10952 nummod 4,%
R3050 T10952 T10953 compound %,paraformaldehyde
R3051 T10953 T10950 pobj paraformaldehyde,with
R3052 T10954 T10953 prep in,paraformaldehyde
R3053 T10955 T10954 pobj PBS,in
R3054 T10956 T10949 punct ", ",perfused
R3055 T10957 T10949 cc and,perfused
R3056 T10958 T10949 conj post-fixed,perfused
R3057 T10959 T10958 advmod overnight,post-fixed
R3058 T10960 T10958 prep in,post-fixed
R3059 T10961 T10962 det the,fixative
R3060 T10962 T10960 pobj fixative,in
R3061 T10963 T10962 amod same,fixative
R3062 T10964 T10939 punct .,isolated
R3063 T10966 T10967 nsubjpass Brains,embedded
R3064 T10968 T10967 auxpass were,embedded
R3065 T10969 T10967 prep in,embedded
R3066 T10970 T10969 pobj gelatin,in
R3067 T10971 T10967 punct ", ",embedded
R3068 T10972 T10967 cc and,embedded
R3069 T10973 T10974 nummod 35,μm
R3070 T10974 T10976 nmod μm,sections
R3071 T10975 T10974 punct -,μm
R3072 T10976 T10978 nsubjpass sections,cut
R3073 T10977 T10976 amod sagittal,sections
R3074 T10978 T10967 conj cut,embedded
R3075 T10979 T10978 auxpass were,cut
R3076 T10980 T10978 advcl using,cut
R3077 T10981 T10982 det a,microtome
R3078 T10982 T10980 dobj microtome,using
R3079 T10983 T10982 amod sliding,microtome
R3080 T10984 T10985 punct (,Associates
R3081 T10985 T10982 parataxis Associates,microtome
R3082 T10986 T10985 compound NeuroScience,Associates
R3083 T10987 T10985 punct ", ",Associates
R3084 T10988 T10985 npadvmod http://www.neuroscienceassociates.com,Associates
R3085 T10989 T10985 punct ),Associates
R3086 T10990 T10978 punct .,cut
R3087 T10992 T10993 nsubjpass Sections,placed
R3088 T10994 T10992 prep from,Sections
R3089 T10995 T10996 amod wild,type
R3090 T10996 T10998 nmod type,brains
R3091 T10997 T10996 punct -,type
R3092 T10998 T10994 pobj brains,from
R3093 T10999 T10996 punct ", ",type
R3094 T11000 T10996 conj heterozygote,type
R3095 T11001 T11000 punct ", ",heterozygote
R3096 T11002 T11000 cc and,heterozygote
R3097 T11003 T11000 conj homozygous,heterozygote
R3098 T11004 T10993 auxpass were,placed
R3099 T11005 T10993 prep in,placed
R3100 T11006 T11007 det the,template
R3101 T11007 T11005 pobj template,in
R3102 T11008 T11007 compound MultiBrain,template
R3103 T11009 T10993 punct .,placed
R3104 T11011 T11012 nsubjpass Sections,washed
R3105 T11013 T11012 auxpass were,washed
R3106 T11014 T11012 prep in,washed
R3107 T11015 T11016 nummod 1,PBS
R3108 T11016 T11014 pobj PBS,in
R3109 T11017 T11015 punct ×,1
R3110 T11018 T11019 advmod prior,to
R3111 T11019 T11012 prep to,washed
R3112 T11020 T11021 nummod 1,h
R3113 T11021 T11019 pobj h,to
R3114 T11022 T11021 prep of,h
R3115 T11023 T11022 pobj incubation,of
R3116 T11024 T11023 prep in,incubation
R3117 T11025 T11026 compound block,solution
R3118 T11026 T11024 pobj solution,in
R3119 T11027 T11026 acl containing,solution
R3120 T11028 T11029 nummod 1,PBS
R3121 T11029 T11027 dobj PBS,containing
R3122 T11030 T11028 punct ×,1
R3123 T11031 T11029 prep with,PBS
R3124 T11032 T11033 nummod 20,%
R3125 T11033 T11034 nmod %,serum
R3126 T11034 T11031 pobj serum,with
R3127 T11035 T11034 amod normal,serum
R3128 T11036 T11034 compound goat,serum
R3129 T11037 T11034 cc and,serum
R3130 T11038 T11039 nummod 0.3,%
R3131 T11039 T11040 compound %,X
R3132 T11040 T11034 conj X,serum
R3133 T11041 T11040 compound Triton,X
R3134 T11042 T11040 punct -,X
R3135 T11043 T11040 nummod 100,X
R3136 T11044 T11045 punct (,pH
R3137 T11045 T11040 parataxis pH,X
R3138 T11046 T11045 nummod 7.4,pH
R3139 T11047 T11045 punct ),pH
R3140 T11048 T11012 punct .,washed
R3141 T11050 T11051 nsubjpass Sections,incubated
R3142 T11052 T11051 auxpass were,incubated
R3143 T11053 T11051 advmod overnight,incubated
R3144 T11054 T11051 prep at,incubated
R3145 T11055 T11056 nummod 4,°C
R3146 T11056 T11054 pobj °C,at
R3147 T11057 T11051 prep in,incubated
R3148 T11058 T11059 amod primary,antibodies
R3149 T11059 T11057 pobj antibodies,in
R3150 T11060 T11059 punct : ,antibodies
R3151 T11061 T11062 nmod affinity,antibody
R3152 T11062 T11059 appos antibody,antibodies
R3153 T11063 T11062 amod purified,antibody
R3154 T11064 T11062 amod polyclonal,antibody
R3155 T11065 T11062 compound Itpr1,antibody
R3156 T11066 T11067 punct (,International
R3157 T11067 T11062 parataxis International,antibody
R3158 T11068 T11067 dep 1,International
R3159 T11069 T11070 punct :,"2,000"
R3160 T11070 T11068 prep "2,000",1
R3161 T11071 T11067 punct ", ",International
R3162 T11072 T11067 compound Chemicon,International
R3163 T11073 T11067 punct ", ",International
R3164 T11074 T11067 npadvmod http://www.chemicon.com,International
R3165 T11075 T11067 punct ),International
R3166 T11076 T11062 cc and,antibody
R3167 T11077 T11078 amod monoclonal,antibody
R3168 T11078 T11062 conj antibody,antibody
R3169 T11079 T11078 amod anti-Calb1,antibody
R3170 T11080 T11081 punct (,Aldrich
R3171 T11081 T11078 parataxis Aldrich,antibody
R3172 T11082 T11081 dep 1,Aldrich
R3173 T11083 T11084 punct :,"6,000"
R3174 T11084 T11082 prep "6,000",1
R3175 T11085 T11081 punct ", ",Aldrich
R3176 T11086 T11081 compound Sigma,Aldrich
R3177 T11087 T11081 punct -,Aldrich
R3178 T11088 T11081 punct ", ",Aldrich
R3179 T11089 T11081 npadvmod http://www.sigmaaldrich.com,Aldrich
R3180 T11090 T11081 punct ),Aldrich
R3181 T11091 T11062 acl diluted,antibody
R3182 T11092 T11091 prep in,diluted
R3183 T11093 T11094 compound carrier,solution
R3184 T11094 T11092 pobj solution,in
R3185 T11095 T11051 punct .,incubated
R3186 T11097 T11098 prep Following,incubated
R3187 T11099 T11100 amod extensive,washes
R3188 T11100 T11097 pobj washes,Following
R3189 T11101 T11100 punct (,washes
R3190 T11102 T11100 prep in,washes
R3191 T11103 T11104 nummod 6.0,ml
R3192 T11104 T11102 pobj ml,in
R3193 T11105 T11104 prep of,ml
R3194 T11106 T11105 pobj PBS,of
R3195 T11107 T11108 punct ", ",times
R3196 T11108 T11100 parataxis times,washes
R3197 T11109 T11108 nummod three,times
R3198 T11110 T11108 punct ),times
R3199 T11111 T11098 punct ", ",incubated
R3200 T11112 T11098 nsubjpass sections,incubated
R3201 T11113 T11098 auxpass were,incubated
R3202 T11114 T11098 prep with,incubated
R3203 T11115 T11116 amod appropriate,antibodies
R3204 T11116 T11114 pobj antibodies,with
R3205 T11117 T11116 amod secondary,antibodies
R3206 T11118 T11119 punct (,IgG
R3207 T11119 T11116 parataxis IgG,antibodies
R3208 T11120 T11121 nmod Alexa,Fluor
R3209 T11121 T11119 nmod Fluor,IgG
R3210 T11122 T11121 nummod 555,Fluor
R3211 T11123 T11119 nmod goat,IgG
R3212 T11124 T11119 amod anti-rabbit,IgG
R3213 T11125 T11119 cc and,IgG
R3214 T11126 T11127 nmod Alexa,Fluor
R3215 T11127 T11128 nmod Fluor,IgG
R3216 T11128 T11119 conj IgG,IgG
R3217 T11129 T11127 nummod 488,Fluor
R3218 T11130 T11128 nmod goat,IgG
R3219 T11131 T11128 amod anti-mouse,IgG
R3220 T11132 T11133 punct [,Invitrogen
R3221 T11133 T11128 parataxis Invitrogen,IgG
R3222 T11134 T11133 punct ", ",Invitrogen
R3223 T11135 T11133 npadvmod http://www.invitrogen.com,Invitrogen
R3224 T11136 T11133 punct ],Invitrogen
R3225 T11137 T11119 punct ),IgG
R3226 T11138 T11098 prep for,incubated
R3227 T11139 T11140 nummod 1,h
R3228 T11140 T11138 pobj h,for
R3229 T11141 T11098 prep at,incubated
R3230 T11142 T11143 compound room,temperature
R3231 T11143 T11141 pobj temperature,at
R3232 T11144 T11098 punct .,incubated
R3233 T11146 T11147 nsubjpass Sections,washed
R3234 T11148 T11147 auxpass were,washed
R3235 T11149 T11147 cc and,washed
R3236 T11150 T11147 conj mounted,washed
R3237 T11151 T11150 prep on,mounted
R3238 T11152 T11153 compound glass,slides
R3239 T11153 T11151 pobj slides,on
R3240 T11154 T11150 prep in,mounted
R3241 T11155 T11156 det a,medium
R3242 T11156 T11154 pobj medium,in
R3243 T11157 T11156 amod buffered,medium
R3244 T11158 T11156 acl containing,medium
R3245 T11159 T11158 dobj Mowiol,containing
R3246 T11160 T11161 punct (,Calbiochem
R3247 T11161 T11159 parataxis Calbiochem,Mowiol
R3248 T11162 T11161 punct ", ",Calbiochem
R3249 T11163 T11161 npadvmod http://www.emdbiosciences.com,Calbiochem
R3250 T11164 T11161 punct ),Calbiochem
R3251 T11165 T11166 mark as,described
R3252 T11166 T11150 advcl described,mounted
R3253 T11167 T11166 advmod earlier,described
R3254 T11168 T11169 punct [,14
R3255 T11169 T11150 parataxis 14,mounted
R3256 T11170 T11169 punct ],14
R3257 T11171 T11147 punct .,washed
R3258 T11173 T11174 nsubjpass Sections,imaged
R3259 T11175 T11174 auxpass were,imaged
R3260 T11176 T11174 advcl using,imaged
R3261 T11177 T11178 det a,microscope
R3262 T11178 T11176 dobj microscope,using
R3263 T11179 T11178 nmod laser,microscope
R3264 T11180 T11178 amod scanning,microscope
R3265 T11181 T11178 amod confocal,microscope
R3266 T11182 T11183 punct (,Zeiss
R3267 T11183 T11178 parataxis Zeiss,microscope
R3268 T11184 T11183 dep LSM,Zeiss
R3269 T11185 T11184 nummod 510,LSM
R3270 T11186 T11183 punct ;,Zeiss
R3271 T11187 T11183 punct ", ",Zeiss
R3272 T11188 T11183 npadvmod http://www.zeiss.com,Zeiss
R3273 T11189 T11183 punct ),Zeiss
R3274 T11190 T11174 punct .,imaged
R3275 T11192 T11193 compound Imaging,parameters
R3276 T11193 T11194 nsubjpass parameters,optimized
R3277 T11195 T11196 punct (,pinhole
R3278 T11196 T11193 parataxis pinhole,parameters
R3279 T11197 T11196 punct ", ",pinhole
R3280 T11198 T11199 compound detector,gain
R3281 T11199 T11196 appos gain,pinhole
R3282 T11200 T11196 punct ", ",pinhole
R3283 T11201 T11202 compound laser,power
R3284 T11202 T11196 appos power,pinhole
R3285 T11203 T11196 punct ),pinhole
R3286 T11204 T11194 auxpass were,optimized
R3287 T11205 T11194 punct ", ",optimized
R3288 T11206 T11194 cc and,optimized
R3289 T11207 T11208 auxpass were,kept
R3290 T11208 T11194 conj kept,optimized
R3291 T11209 T11208 oprd constant,kept
R3292 T11210 T11208 prep for,kept
R3293 T11211 T11212 det the,brains
R3294 T11212 T11210 pobj brains,for
R3295 T11213 T11214 amod wild,type
R3296 T11214 T11212 nmod type,brains
R3297 T11215 T11214 punct -,type
R3298 T11216 T11214 punct ", ",type
R3299 T11217 T11214 conj heterozygous,type
R3300 T11218 T11217 punct ", ",heterozygous
R3301 T11219 T11217 cc and,heterozygous
R3302 T11220 T11217 conj homozygous,heterozygous
R3303 T11221 T11212 compound mutant,brains
R3304 T11222 T11194 punct .,optimized
R3305 T11224 T11225 nsubjpass Specificity,verified
R3306 T11226 T11224 prep of,Specificity
R3307 T11227 T11228 det the,antibodies
R3308 T11228 T11226 pobj antibodies,of
R3309 T11229 T11228 compound Itpr1,antibodies
R3310 T11230 T11225 auxpass was,verified
R3311 T11231 T11225 prep by,verified
R3312 T11232 T11233 compound preabsorption,experiments
R3313 T11233 T11231 pobj experiments,by
R3314 T11234 T11233 compound control,experiments
R3315 T11235 T11225 punct .,verified
R3316 T11237 T11238 compound Antibody,dilutions
R3317 T11238 T11239 nsubjpass dilutions,incubated
R3318 T11240 T11239 auxpass were,incubated
R3319 T11241 T11239 prep for,incubated
R3320 T11242 T11243 nummod 24,h
R3321 T11243 T11241 pobj h,for
R3322 T11244 T11239 prep at,incubated
R3323 T11245 T11246 nummod 4,°C
R3324 T11246 T11244 pobj °C,at
R3325 T11247 T11239 prep with,incubated
R3326 T11248 T11249 det the,peptide
R3327 T11249 T11247 pobj peptide,with
R3328 T11250 T11249 amod immunizing,peptide
R3329 T11251 T11239 punct .,incubated
R3330 T11253 T11254 compound Tissue,sections
R3331 T11254 T11255 nsubjpass sections,incubated
R3332 T11256 T11255 auxpass were,incubated
R3333 T11257 T11255 prep with,incubated
R3334 T11258 T11259 det the,antibodies
R3335 T11259 T11257 pobj antibodies,with
R3336 T11260 T11259 amod preabsorbed,antibodies
R3337 T11261 T11255 cc and,incubated
R3338 T11262 T11255 conj processed,incubated
R3339 T11263 T11264 mark as,described
R3340 T11264 T11262 advcl described,processed
R3341 T11265 T11264 advmod above,described
R3342 T11266 T11255 punct .,incubated
R3343 T11268 T11269 prep Under,detected
R3344 T11270 T11271 det these,conditions
R3345 T11271 T11268 pobj conditions,Under
R3346 T11272 T11269 punct ", ",detected
R3347 T11273 T11274 det no,staining
R3348 T11274 T11269 nsubjpass staining,detected
R3349 T11275 T11274 prep above,staining
R3350 T11276 T11275 pobj autofluorescence,above
R3351 T11277 T11269 auxpass was,detected
R3352 T11278 T11269 punct .,detected