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PMC:1892049 / 21236-21945 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T10116 0-8 NN denotes Analysis
T10117 9-11 IN denotes of
T10118 12-17 NN denotes Itpr1
T10119 18-25 NN denotes protein
T10120 26-28 IN denotes in
T10121 29-33 NNS denotes mice
T10122 33-34 . denotes .
T10123 34-156 sentence denotes We performed Western blot analyses using standard techniques with ECL detection kits (Amersham, http://www.amersham.com).
T10124 35-37 PRP denotes We
T10125 38-47 VBD denotes performed
T10126 48-55 NNP denotes Western
T10127 56-60 NN denotes blot
T10128 61-69 NNS denotes analyses
T10129 70-75 VBG denotes using
T10130 76-84 JJ denotes standard
T10131 85-95 NNS denotes techniques
T10132 96-100 IN denotes with
T10133 101-104 NN denotes ECL
T10135 105-114 NN denotes detection
T10134 115-119 NNS denotes kits
T10136 120-121 -LRB- denotes (
T10137 121-129 NNP denotes Amersham
T10138 129-131 , denotes ,
T10139 131-154 NN denotes http://www.amersham.com
T10140 154-155 -RRB- denotes )
T10141 155-156 . denotes .
T10142 156-422 sentence denotes Briefly, dissected whole brains from postnatal day 21 littermates were homogenized in a buffer containing 50 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, and a cocktail of protease inhibitors (Roche, http://www.roche.com).
T10143 157-164 RB denotes Briefly
T10145 164-166 , denotes ,
T10146 166-175 VBN denotes dissected
T10148 176-181 JJ denotes whole
T10147 182-188 NNS denotes brains
T10149 189-193 IN denotes from
T10150 194-203 JJ denotes postnatal
T10151 204-207 NN denotes day
T10153 208-210 CD denotes 21
T10152 211-222 NNS denotes littermates
T10154 223-227 VBD denotes were
T10144 228-239 VBN denotes homogenized
T10155 240-242 IN denotes in
T10156 243-244 DT denotes a
T10157 245-251 NN denotes buffer
T10158 252-262 VBG denotes containing
T10159 263-265 CD denotes 50
T10160 266-268 NN denotes mM
T10162 269-273 NN denotes Tris
T10163 273-274 HYPH denotes -
T10161 274-277 NN denotes HCl
T10164 277-279 , denotes ,
T10165 279-282 CD denotes 150
T10166 283-285 NN denotes mM
T10167 286-290 NN denotes NaCl
T10168 290-292 , denotes ,
T10169 292-293 CD denotes 1
T10170 294-296 NN denotes mM
T10171 297-301 NN denotes EDTA
T10172 301-303 , denotes ,
T10173 303-304 CD denotes 1
T10174 304-305 NN denotes %
T10176 306-312 NN denotes Triton
T10175 313-314 NN denotes X
T10177 314-315 HYPH denotes -
T10178 315-318 CD denotes 100
T10179 318-320 , denotes ,
T10180 320-321 CD denotes 1
T10181 321-322 NN denotes %
T10183 323-329 NN denotes sodium
T10182 330-342 NN denotes deoxycholate
T10184 342-344 , denotes ,
T10185 344-347 CD denotes 0.1
T10186 347-348 NN denotes %
T10187 349-352 NN denotes SDS
T10188 352-354 , denotes ,
T10189 354-357 CC denotes and
T10190 358-359 DT denotes a
T10191 360-368 NN denotes cocktail
T10192 369-371 IN denotes of
T10193 372-380 NN denotes protease
T10194 381-391 NNS denotes inhibitors
T10195 392-393 -LRB- denotes (
T10196 393-398 NNP denotes Roche
T10197 398-400 , denotes ,
T10198 400-420 NN denotes http://www.roche.com
T10199 420-421 -RRB- denotes )
T10200 421-422 . denotes .
T10201 422-613 sentence denotes Homogenates were diluted appropriately, mixed with 4× reducing sample buffer, and loaded onto 4%–12% precast gradient gels (Novex, http://www.invitrogen.com) for SDS-PAGE and immunoblotting.
T10202 423-434 NNS denotes Homogenates
T10204 435-439 VBD denotes were
T10203 440-447 VBN denotes diluted
T10205 448-461 RB denotes appropriately
T10206 461-463 , denotes ,
T10207 463-468 VBN denotes mixed
T10208 469-473 IN denotes with
T10209 474-475 CD denotes 4
T10211 475-476 SYM denotes ×
T10212 477-485 VBG denotes reducing
T10213 486-492 NN denotes sample
T10210 493-499 NN denotes buffer
T10214 499-501 , denotes ,
T10215 501-504 CC denotes and
T10216 505-511 VBN denotes loaded
T10217 512-516 IN denotes onto
T10218 517-518 CD denotes 4
T10219 518-519 NN denotes %
T10221 519-520 SYM denotes –
T10223 520-522 CD denotes 12
T10222 522-523 NN denotes %
T10224 524-531 NN denotes precast
T10225 532-540 NN denotes gradient
T10220 541-545 NNS denotes gels
T10226 546-547 -LRB- denotes (
T10227 547-552 NNP denotes Novex
T10228 552-554 , denotes ,
T10229 554-579 NN denotes http://www.invitrogen.com
T10230 579-580 -RRB- denotes )
T10231 581-584 IN denotes for
T10232 585-588 NN denotes SDS
T10234 588-589 HYPH denotes -
T10233 589-593 NN denotes PAGE
T10235 594-597 CC denotes and
T10236 598-612 NN denotes immunoblotting
T10237 612-613 . denotes .
T10238 613-709 sentence denotes The antibodies to Itpr1 (1:2,000) and Actb (1:5,000) were used as recommended by manufacturers.
T10239 614-617 DT denotes The
T10240 618-628 NNS denotes antibodies
T10242 629-631 IN denotes to
T10243 632-637 NN denotes Itpr1
T10244 638-639 -LRB- denotes (
T10245 639-640 CD denotes 1
T10246 640-641 SYM denotes :
T10247 641-646 CD denotes 2,000
T10248 646-647 -RRB- denotes )
T10249 648-651 CC denotes and
T10250 652-656 NN denotes Actb
T10251 657-658 -LRB- denotes (
T10252 658-659 CD denotes 1
T10253 659-660 SYM denotes :
T10254 660-665 CD denotes 5,000
T10255 665-666 -RRB- denotes )
T10256 667-671 VBD denotes were
T10241 672-676 VBN denotes used
T10257 677-679 IN denotes as
T10258 680-691 VBN denotes recommended
T10259 692-694 IN denotes by
T10260 695-708 NNS denotes manufacturers
T10261 708-709 . denotes .
R2899 T10117 T10116 prep of,Analysis
R2900 T10118 T10119 compound Itpr1,protein
R2901 T10119 T10117 pobj protein,of
R2902 T10120 T10119 prep in,protein
R2903 T10121 T10120 pobj mice,in
R2904 T10122 T10116 punct .,Analysis
R2905 T10124 T10125 nsubj We,performed
R2906 T10126 T10127 compound Western,blot
R2907 T10127 T10128 compound blot,analyses
R2908 T10128 T10125 dobj analyses,performed
R2909 T10129 T10125 advcl using,performed
R2910 T10130 T10131 amod standard,techniques
R2911 T10131 T10129 dobj techniques,using
R2912 T10132 T10125 prep with,performed
R2913 T10133 T10134 compound ECL,kits
R2914 T10134 T10132 pobj kits,with
R2915 T10135 T10134 compound detection,kits
R2916 T10136 T10137 punct (,Amersham
R2917 T10137 T10134 parataxis Amersham,kits
R2918 T10138 T10137 punct ", ",Amersham
R2919 T10139 T10137 npadvmod http://www.amersham.com,Amersham
R2920 T10140 T10137 punct ),Amersham
R2921 T10141 T10125 punct .,performed
R2922 T10143 T10144 advmod Briefly,homogenized
R2923 T10145 T10144 punct ", ",homogenized
R2924 T10146 T10147 amod dissected,brains
R2925 T10147 T10144 nsubjpass brains,homogenized
R2926 T10148 T10147 amod whole,brains
R2927 T10149 T10147 prep from,brains
R2928 T10150 T10151 amod postnatal,day
R2929 T10151 T10152 nmod day,littermates
R2930 T10152 T10149 pobj littermates,from
R2931 T10153 T10151 nummod 21,day
R2932 T10154 T10144 auxpass were,homogenized
R2933 T10155 T10144 prep in,homogenized
R2934 T10156 T10157 det a,buffer
R2935 T10157 T10155 pobj buffer,in
R2936 T10158 T10157 acl containing,buffer
R2937 T10159 T10160 nummod 50,mM
R2938 T10160 T10161 compound mM,HCl
R2939 T10161 T10158 dobj HCl,containing
R2940 T10162 T10161 compound Tris,HCl
R2941 T10163 T10161 punct -,HCl
R2942 T10164 T10161 punct ", ",HCl
R2943 T10165 T10166 nummod 150,mM
R2944 T10166 T10167 compound mM,NaCl
R2945 T10167 T10161 conj NaCl,HCl
R2946 T10168 T10167 punct ", ",NaCl
R2947 T10169 T10170 nummod 1,mM
R2948 T10170 T10171 compound mM,EDTA
R2949 T10171 T10167 conj EDTA,NaCl
R2950 T10172 T10171 punct ", ",EDTA
R2951 T10173 T10174 nummod 1,%
R2952 T10174 T10175 compound %,X
R2953 T10175 T10171 conj X,EDTA
R2954 T10176 T10175 compound Triton,X
R2955 T10177 T10175 punct -,X
R2956 T10178 T10175 nummod 100,X
R2957 T10179 T10175 punct ", ",X
R2958 T10180 T10181 nummod 1,%
R2959 T10181 T10182 compound %,deoxycholate
R2960 T10182 T10175 conj deoxycholate,X
R2961 T10183 T10182 compound sodium,deoxycholate
R2962 T10184 T10182 punct ", ",deoxycholate
R2963 T10185 T10186 nummod 0.1,%
R2964 T10186 T10187 compound %,SDS
R2965 T10187 T10182 conj SDS,deoxycholate
R2966 T10188 T10187 punct ", ",SDS
R2967 T10189 T10187 cc and,SDS
R2968 T10190 T10191 det a,cocktail
R2969 T10191 T10187 conj cocktail,SDS
R2970 T10192 T10191 prep of,cocktail
R2971 T10193 T10194 compound protease,inhibitors
R2972 T10194 T10192 pobj inhibitors,of
R2973 T10195 T10196 punct (,Roche
R2974 T10196 T10191 parataxis Roche,cocktail
R2975 T10197 T10196 punct ", ",Roche
R2976 T10198 T10196 npadvmod http://www.roche.com,Roche
R2977 T10199 T10196 punct ),Roche
R2978 T10200 T10144 punct .,homogenized
R2979 T10202 T10203 nsubjpass Homogenates,diluted
R2980 T10204 T10203 auxpass were,diluted
R2981 T10205 T10203 advmod appropriately,diluted
R2982 T10206 T10203 punct ", ",diluted
R2983 T10207 T10203 conj mixed,diluted
R2984 T10208 T10207 prep with,mixed
R2985 T10209 T10210 nummod 4,buffer
R2986 T10210 T10208 pobj buffer,with
R2987 T10211 T10209 punct ×,4
R2988 T10212 T10210 amod reducing,buffer
R2989 T10213 T10210 compound sample,buffer
R2990 T10214 T10207 punct ", ",mixed
R2991 T10215 T10207 cc and,mixed
R2992 T10216 T10207 conj loaded,mixed
R2993 T10217 T10216 prep onto,loaded
R2994 T10218 T10219 nummod 4,%
R2995 T10219 T10220 nmod %,gels
R2996 T10220 T10217 pobj gels,onto
R2997 T10221 T10222 punct –,%
R2998 T10222 T10219 prep %,%
R2999 T10223 T10222 nummod 12,%
R3000 T10224 T10220 compound precast,gels
R3001 T10225 T10220 compound gradient,gels
R3002 T10226 T10227 punct (,Novex
R3003 T10227 T10220 parataxis Novex,gels
R3004 T10228 T10227 punct ", ",Novex
R3005 T10229 T10227 npadvmod http://www.invitrogen.com,Novex
R3006 T10230 T10227 punct ),Novex
R3007 T10231 T10216 prep for,loaded
R3008 T10232 T10233 compound SDS,PAGE
R3009 T10233 T10231 pobj PAGE,for
R3010 T10234 T10233 punct -,PAGE
R3011 T10235 T10233 cc and,PAGE
R3012 T10236 T10233 conj immunoblotting,PAGE
R3013 T10237 T10203 punct .,diluted
R3014 T10239 T10240 det The,antibodies
R3015 T10240 T10241 nsubjpass antibodies,used
R3016 T10242 T10240 prep to,antibodies
R3017 T10243 T10242 pobj Itpr1,to
R3018 T10244 T10245 punct (,1
R3019 T10245 T10243 parataxis 1,Itpr1
R3020 T10246 T10247 punct :,"2,000"
R3021 T10247 T10245 prep "2,000",1
R3022 T10248 T10245 punct ),1
R3023 T10249 T10243 cc and,Itpr1
R3024 T10250 T10243 conj Actb,Itpr1
R3025 T10251 T10252 punct (,1
R3026 T10252 T10250 parataxis 1,Actb
R3027 T10253 T10254 punct :,"5,000"
R3028 T10254 T10252 prep "5,000",1
R3029 T10255 T10252 punct ),1
R3030 T10256 T10241 auxpass were,used
R3031 T10257 T10258 mark as,recommended
R3032 T10258 T10241 advcl recommended,used
R3033 T10259 T10258 agent by,recommended
R3034 T10260 T10259 pobj manufacturers,by
R3035 T10261 T10241 punct .,used