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PMC:1860061 / 8242-8963 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T5000 0-6 NN denotes Sample
T5001 7-18 NN denotes preparation
T5002 19-22 CC denotes and
T5003 23-33 NN denotes microarray
T5004 34-47 NN denotes hybridisation
T5005 47-133 sentence denotes Lymph nodes (LNs) draining the immunisation site were used for total RNA preparation.
T5006 48-53 NN denotes Lymph
T5007 54-59 NNS denotes nodes
T5009 60-61 -LRB- denotes (
T5010 61-64 NNS denotes LNs
T5011 64-65 -RRB- denotes )
T5012 66-74 VBG denotes draining
T5013 75-78 DT denotes the
T5015 79-91 NN denotes immunisation
T5014 92-96 NN denotes site
T5016 97-101 VBD denotes were
T5008 102-106 VBN denotes used
T5017 107-110 IN denotes for
T5018 111-116 JJ denotes total
T5020 117-120 NN denotes RNA
T5019 121-132 NN denotes preparation
T5021 132-133 . denotes .
T5022 133-279 sentence denotes The total RNA was extracted from the tissue homogenates using a commercial kit in accordance with the provided protocol (QIGEN, Hilden, Germany).
T5023 134-137 DT denotes The
T5025 138-143 JJ denotes total
T5024 144-147 NN denotes RNA
T5027 148-151 VBD denotes was
T5026 152-161 VBN denotes extracted
T5028 162-166 IN denotes from
T5029 167-170 DT denotes the
T5031 171-177 NN denotes tissue
T5030 178-189 NNS denotes homogenates
T5032 190-195 VBG denotes using
T5033 196-197 DT denotes a
T5035 198-208 JJ denotes commercial
T5034 209-212 NN denotes kit
T5036 213-215 IN denotes in
T5037 216-226 NN denotes accordance
T5038 227-231 IN denotes with
T5039 232-235 DT denotes the
T5041 236-244 VBN denotes provided
T5040 245-253 NN denotes protocol
T5042 254-255 -LRB- denotes (
T5043 255-260 NNP denotes QIGEN
T5044 260-262 , denotes ,
T5045 262-268 NNP denotes Hilden
T5046 268-270 , denotes ,
T5047 270-277 NNP denotes Germany
T5048 277-278 -RRB- denotes )
T5049 278-279 . denotes .
T5050 279-414 sentence denotes Analysis of gene expression was conducted using a U430A array (Affymetrix, Santa Clara, CA, USA) interrogating more than 22,000 genes.
T5051 280-288 NN denotes Analysis
T5053 289-291 IN denotes of
T5054 292-296 NN denotes gene
T5055 297-307 NN denotes expression
T5056 308-311 VBD denotes was
T5052 312-321 VBN denotes conducted
T5057 322-327 VBG denotes using
T5058 328-329 DT denotes a
T5060 330-335 NN denotes U430A
T5059 336-341 NN denotes array
T5061 342-343 -LRB- denotes (
T5062 343-353 NNP denotes Affymetrix
T5063 353-355 , denotes ,
T5064 355-360 NNP denotes Santa
T5065 361-366 NNP denotes Clara
T5066 366-368 , denotes ,
T5067 368-370 NNP denotes CA
T5068 370-372 , denotes ,
T5069 372-375 NNP denotes USA
T5070 375-376 -RRB- denotes )
T5071 377-390 VBG denotes interrogating
T5072 391-395 JJR denotes more
T5074 396-400 IN denotes than
T5073 401-407 CD denotes 22,000
T5075 408-413 NNS denotes genes
T5076 413-414 . denotes .
T5077 414-491 sentence denotes RNA probes were labelled in accordance with the manufacturer's instructions.
T5078 415-418 NN denotes RNA
T5079 419-425 NNS denotes probes
T5081 426-430 VBD denotes were
T5080 431-439 VBN denotes labelled
T5082 440-442 IN denotes in
T5083 443-453 NN denotes accordance
T5084 454-458 IN denotes with
T5085 459-462 DT denotes the
T5086 463-475 NN denotes manufacturer
T5088 475-477 POS denotes 's
T5087 478-490 NNS denotes instructions
T5089 490-491 . denotes .
T5090 491-560 sentence denotes Samples from individual mice were hybridised onto individual arrays.
T5091 492-499 NNS denotes Samples
T5093 500-504 IN denotes from
T5094 505-515 JJ denotes individual
T5095 516-520 NNS denotes mice
T5096 521-525 VBD denotes were
T5092 526-536 VBN denotes hybridised
T5097 537-541 IN denotes onto
T5098 542-552 JJ denotes individual
T5099 553-559 NNS denotes arrays
T5100 559-560 . denotes .
T5101 560-640 sentence denotes Hybridisation and washing of gene chips were done as previously described [16].
T5102 561-574 NN denotes Hybridisation
T5104 575-578 CC denotes and
T5105 579-586 NN denotes washing
T5106 587-589 IN denotes of
T5107 590-594 NN denotes gene
T5108 595-600 NNS denotes chips
T5109 601-605 VBD denotes were
T5103 606-610 VBN denotes done
T5110 611-613 IN denotes as
T5112 614-624 RB denotes previously
T5111 625-634 VBN denotes described
T5113 635-636 -LRB- denotes [
T5114 636-638 CD denotes 16
T5115 638-639 -RRB- denotes ]
T5116 639-640 . denotes .
T5117 640-721 sentence denotes Fluorescent signals were collected by laser scan (Hewlett-Packard Gene Scanner).
T5118 641-652 JJ denotes Fluorescent
T5119 653-660 NNS denotes signals
T5121 661-665 VBD denotes were
T5120 666-675 VBN denotes collected
T5122 676-678 IN denotes by
T5123 679-684 NN denotes laser
T5124 685-689 NN denotes scan
T5125 690-691 -LRB- denotes (
T5127 691-698 NNP denotes Hewlett
T5129 698-699 HYPH denotes -
T5128 699-706 NNP denotes Packard
T5130 707-711 NNP denotes Gene
T5126 712-719 NNP denotes Scanner
T5131 719-720 -RRB- denotes )
T5132 720-721 . denotes .
R1478 T5000 T5001 compound Sample,preparation
R1479 T5002 T5001 cc and,preparation
R1480 T5003 T5004 compound microarray,hybridisation
R1481 T5004 T5001 conj hybridisation,preparation
R1482 T5006 T5007 compound Lymph,nodes
R1483 T5007 T5008 nsubjpass nodes,used
R1484 T5009 T5007 punct (,nodes
R1485 T5010 T5007 appos LNs,nodes
R1486 T5011 T5007 punct ),nodes
R1487 T5012 T5007 acl draining,nodes
R1488 T5013 T5014 det the,site
R1489 T5014 T5012 dobj site,draining
R1490 T5015 T5014 compound immunisation,site
R1491 T5016 T5008 auxpass were,used
R1492 T5017 T5008 prep for,used
R1493 T5018 T5019 amod total,preparation
R1494 T5019 T5017 pobj preparation,for
R1495 T5020 T5019 compound RNA,preparation
R1496 T5021 T5008 punct .,used
R1497 T5023 T5024 det The,RNA
R1498 T5024 T5026 nsubjpass RNA,extracted
R1499 T5025 T5024 amod total,RNA
R1500 T5027 T5026 auxpass was,extracted
R1501 T5028 T5026 prep from,extracted
R1502 T5029 T5030 det the,homogenates
R1503 T5030 T5028 pobj homogenates,from
R1504 T5031 T5030 compound tissue,homogenates
R1505 T5032 T5026 advcl using,extracted
R1506 T5033 T5034 det a,kit
R1507 T5034 T5032 dobj kit,using
R1508 T5035 T5034 amod commercial,kit
R1509 T5036 T5026 prep in,extracted
R1510 T5037 T5036 pobj accordance,in
R1511 T5038 T5037 prep with,accordance
R1512 T5039 T5040 det the,protocol
R1513 T5040 T5038 pobj protocol,with
R1514 T5041 T5040 amod provided,protocol
R1515 T5042 T5043 punct (,QIGEN
R1516 T5043 T5026 parataxis QIGEN,extracted
R1517 T5044 T5043 punct ", ",QIGEN
R1518 T5045 T5043 npadvmod Hilden,QIGEN
R1519 T5046 T5043 punct ", ",QIGEN
R1520 T5047 T5043 npadvmod Germany,QIGEN
R1521 T5048 T5043 punct ),QIGEN
R1522 T5049 T5026 punct .,extracted
R1523 T5051 T5052 nsubjpass Analysis,conducted
R1524 T5053 T5051 prep of,Analysis
R1525 T5054 T5055 compound gene,expression
R1526 T5055 T5053 pobj expression,of
R1527 T5056 T5052 auxpass was,conducted
R1528 T5057 T5052 advcl using,conducted
R1529 T5058 T5059 det a,array
R1530 T5059 T5057 dobj array,using
R1531 T5060 T5059 compound U430A,array
R1532 T5061 T5062 punct (,Affymetrix
R1533 T5062 T5059 parataxis Affymetrix,array
R1534 T5063 T5062 punct ", ",Affymetrix
R1535 T5064 T5065 compound Santa,Clara
R1536 T5065 T5062 npadvmod Clara,Affymetrix
R1537 T5066 T5062 punct ", ",Affymetrix
R1538 T5067 T5062 npadvmod CA,Affymetrix
R1539 T5068 T5062 punct ", ",Affymetrix
R1540 T5069 T5062 npadvmod USA,Affymetrix
R1541 T5070 T5062 punct ),Affymetrix
R1542 T5071 T5059 acl interrogating,array
R1543 T5072 T5073 amod more,"22,000"
R1544 T5073 T5075 nummod "22,000",genes
R1545 T5074 T5073 quantmod than,"22,000"
R1546 T5075 T5071 dobj genes,interrogating
R1547 T5076 T5052 punct .,conducted
R1548 T5078 T5079 compound RNA,probes
R1549 T5079 T5080 nsubjpass probes,labelled
R1550 T5081 T5080 auxpass were,labelled
R1551 T5082 T5080 prep in,labelled
R1552 T5083 T5082 pobj accordance,in
R1553 T5084 T5083 prep with,accordance
R1554 T5085 T5086 det the,manufacturer
R1555 T5086 T5087 poss manufacturer,instructions
R1556 T5087 T5084 pobj instructions,with
R1557 T5088 T5086 case 's,manufacturer
R1558 T5089 T5080 punct .,labelled
R1559 T5091 T5092 nsubjpass Samples,hybridised
R1560 T5093 T5091 prep from,Samples
R1561 T5094 T5095 amod individual,mice
R1562 T5095 T5093 pobj mice,from
R1563 T5096 T5092 auxpass were,hybridised
R1564 T5097 T5092 prep onto,hybridised
R1565 T5098 T5099 amod individual,arrays
R1566 T5099 T5097 pobj arrays,onto
R1567 T5100 T5092 punct .,hybridised
R1568 T5102 T5103 nsubjpass Hybridisation,done
R1569 T5104 T5102 cc and,Hybridisation
R1570 T5105 T5102 conj washing,Hybridisation
R1571 T5106 T5102 prep of,Hybridisation
R1572 T5107 T5108 compound gene,chips
R1573 T5108 T5106 pobj chips,of
R1574 T5109 T5103 auxpass were,done
R1575 T5110 T5111 mark as,described
R1576 T5111 T5103 advcl described,done
R1577 T5112 T5111 advmod previously,described
R1578 T5113 T5114 punct [,16
R1579 T5114 T5103 parataxis 16,done
R1580 T5115 T5114 punct ],16
R1581 T5116 T5103 punct .,done
R1582 T5118 T5119 amod Fluorescent,signals
R1583 T5119 T5120 nsubjpass signals,collected
R1584 T5121 T5120 auxpass were,collected
R1585 T5122 T5120 prep by,collected
R1586 T5123 T5124 compound laser,scan
R1587 T5124 T5122 pobj scan,by
R1588 T5125 T5126 punct (,Scanner
R1589 T5126 T5124 parataxis Scanner,scan
R1590 T5127 T5128 compound Hewlett,Packard
R1591 T5128 T5126 compound Packard,Scanner
R1592 T5129 T5128 punct -,Packard
R1593 T5130 T5126 compound Gene,Scanner
R1594 T5131 T5126 punct ),Scanner
R1595 T5132 T5120 punct .,collected