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PMC:1858683 / 42766-43428 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T20633 0-3 NN denotes BAT
T20635 3-4 HYPH denotes -
T20634 4-7 NN denotes gal
T20636 8-17 NN denotes transgene
T20637 18-26 NN denotes reporter
T20638 27-32 NN denotes assay
T20639 33-35 IN denotes of
T20640 36-45 JJ denotes canonical
T20642 46-49 NN denotes Wnt
T20641 50-59 NN denotes signaling
T20643 59-157 sentence denotes X-gal staining of both embryos and developing kidneys was performed as previously described [21].
T20644 60-61 NN denotes X
T20646 61-62 HYPH denotes -
T20645 62-65 NN denotes gal
T20647 66-74 NN denotes staining
T20649 75-77 IN denotes of
T20650 78-82 CC denotes both
T20651 83-90 NNS denotes embryos
T20652 91-94 CC denotes and
T20653 95-105 VBG denotes developing
T20654 106-113 NNS denotes kidneys
T20655 114-117 VBD denotes was
T20648 118-127 VBN denotes performed
T20656 128-130 IN denotes as
T20658 131-141 RB denotes previously
T20657 142-151 VBN denotes described
T20659 152-153 -LRB- denotes [
T20660 153-155 CD denotes 21
T20661 155-156 -RRB- denotes ]
T20662 156-157 . denotes .
T20663 157-303 sentence denotes Care was taken to reduce endogenous β-galactosidase activity within the developing kidney by increasing pH of the X-gal staining solution to 8.0.
T20664 158-162 NN denotes Care
T20666 163-166 VBD denotes was
T20665 167-172 VBN denotes taken
T20667 173-175 TO denotes to
T20668 176-182 VB denotes reduce
T20669 183-193 JJ denotes endogenous
T20671 194-195 NN denotes β
T20673 195-196 HYPH denotes -
T20672 196-209 NN denotes galactosidase
T20670 210-218 NN denotes activity
T20674 219-225 IN denotes within
T20675 226-229 DT denotes the
T20677 230-240 VBG denotes developing
T20676 241-247 NN denotes kidney
T20678 248-250 IN denotes by
T20679 251-261 VBG denotes increasing
T20680 262-264 NN denotes pH
T20681 265-267 IN denotes of
T20682 268-271 DT denotes the
T20684 272-273 NN denotes X
T20686 273-274 HYPH denotes -
T20685 274-277 NN denotes gal
T20687 278-286 NN denotes staining
T20683 287-295 NN denotes solution
T20688 296-298 IN denotes to
T20689 299-302 CD denotes 8.0
T20690 302-303 . denotes .
T20691 303-477 sentence denotes Changes in transgene β-Gal expression were quantitated using a β-Gal ELISA (Enzyme-linked immunoassay) kit (Roche, Indianapolis, IN), normalizing according to total protein.
T20692 304-311 NNS denotes Changes
T20694 312-314 IN denotes in
T20695 315-324 NN denotes transgene
T20697 325-326 NN denotes β
T20698 326-327 HYPH denotes -
T20696 327-330 NN denotes Gal
T20699 331-341 NN denotes expression
T20700 342-346 VBD denotes were
T20693 347-358 VBN denotes quantitated
T20701 359-364 VBG denotes using
T20702 365-366 DT denotes a
T20704 367-368 NN denotes β
T20706 368-369 HYPH denotes -
T20705 369-372 NN denotes Gal
T20707 373-378 NNP denotes ELISA
T20708 379-380 -LRB- denotes (
T20709 380-386 NN denotes Enzyme
T20711 386-387 HYPH denotes -
T20710 387-393 VBN denotes linked
T20712 394-405 NN denotes immunoassay
T20713 405-406 -RRB- denotes )
T20703 407-410 NN denotes kit
T20714 411-412 -LRB- denotes (
T20715 412-417 NNP denotes Roche
T20716 417-419 , denotes ,
T20717 419-431 NNP denotes Indianapolis
T20718 431-433 , denotes ,
T20719 433-435 NNP denotes IN
T20720 435-436 -RRB- denotes )
T20721 436-438 , denotes ,
T20722 438-449 VBG denotes normalizing
T20723 450-459 VBG denotes according
T20724 460-462 IN denotes to
T20725 463-468 JJ denotes total
T20726 469-476 NN denotes protein
T20727 476-477 . denotes .
T20728 477-662 sentence denotes Each genotype was represented by a sample size of 4 except the non-transgenic (n = 5), Pygo1+/- ; Pygo2+/+ (n = 6), Pygo1-/- ; Pygo2+/- (n = 8), and Pygo1-/- ; Pygo2-/- (n = 6) groups.
T20729 478-482 DT denotes Each
T20730 483-491 NN denotes genotype
T20732 492-495 VBD denotes was
T20731 496-507 VBN denotes represented
T20733 508-510 IN denotes by
T20734 511-512 DT denotes a
T20736 513-519 NN denotes sample
T20735 520-524 NN denotes size
T20737 525-527 IN denotes of
T20738 528-529 CD denotes 4
T20739 530-536 IN denotes except
T20740 537-540 DT denotes the
T20742 541-555 JJ denotes non-transgenic
T20743 556-557 -LRB- denotes (
T20745 557-558 NN denotes n
T20746 559-560 SYM denotes =
T20744 561-562 CD denotes 5
T20747 562-563 -RRB- denotes )
T20748 563-565 , denotes ,
T20749 565-570 NN denotes Pygo1
T20750 570-571 SYM denotes +
T20751 571-572 HYPH denotes /
T20752 572-573 SYM denotes -
T20753 574-575 : denotes ;
T20754 576-581 NN denotes Pygo2
T20755 581-582 SYM denotes +
T20756 582-583 HYPH denotes /
T20757 583-584 SYM denotes +
T20758 585-586 -LRB- denotes (
T20760 586-587 NN denotes n
T20761 588-589 SYM denotes =
T20759 590-591 CD denotes 6
T20762 591-592 -RRB- denotes )
T20763 592-594 , denotes ,
T20764 594-599 NN denotes Pygo1
T20765 599-600 SYM denotes -
T20766 600-601 HYPH denotes /
T20767 601-602 SYM denotes -
T20768 603-604 : denotes ;
T20769 605-610 NN denotes Pygo2
T20770 610-611 SYM denotes +
T20771 611-612 HYPH denotes /
T20772 612-613 SYM denotes -
T20773 614-615 -LRB- denotes (
T20775 615-616 NN denotes n
T20776 617-618 SYM denotes =
T20774 619-620 CD denotes 8
T20777 620-621 -RRB- denotes )
T20778 621-623 , denotes ,
T20779 623-626 CC denotes and
T20780 627-632 NN denotes Pygo1
T20781 632-633 SYM denotes -
T20782 633-634 HYPH denotes /
T20783 634-635 SYM denotes -
T20784 636-637 : denotes ;
T20785 638-643 NN denotes Pygo2
T20786 643-644 SYM denotes -
T20787 644-645 HYPH denotes /
T20788 645-646 SYM denotes -
T20789 647-648 -LRB- denotes (
T20791 648-649 NN denotes n
T20792 650-651 SYM denotes =
T20790 652-653 CD denotes 6
T20793 653-654 -RRB- denotes )
T20741 655-661 NNS denotes groups
T20794 661-662 . denotes .
R5826 T20633 T20634 compound BAT,gal
R5827 T20634 T20636 compound gal,transgene
R5828 T20635 T20634 punct -,gal
R5829 T20636 T20637 compound transgene,reporter
R5830 T20637 T20638 compound reporter,assay
R5831 T20639 T20638 prep of,assay
R5832 T20640 T20641 amod canonical,signaling
R5833 T20641 T20639 pobj signaling,of
R5834 T20642 T20641 compound Wnt,signaling
R5835 T20644 T20645 compound X,gal
R5836 T20645 T20647 compound gal,staining
R5837 T20646 T20645 punct -,gal
R5838 T20647 T20648 nsubjpass staining,performed
R5839 T20649 T20647 prep of,staining
R5840 T20650 T20651 preconj both,embryos
R5841 T20651 T20649 pobj embryos,of
R5842 T20652 T20651 cc and,embryos
R5843 T20653 T20654 amod developing,kidneys
R5844 T20654 T20651 conj kidneys,embryos
R5845 T20655 T20648 auxpass was,performed
R5846 T20656 T20657 mark as,described
R5847 T20657 T20648 advcl described,performed
R5848 T20658 T20657 advmod previously,described
R5849 T20659 T20660 punct [,21
R5850 T20660 T20657 parataxis 21,described
R5851 T20661 T20660 punct ],21
R5852 T20662 T20648 punct .,performed
R5853 T20664 T20665 nsubjpass Care,taken
R5854 T20666 T20665 auxpass was,taken
R5855 T20667 T20668 aux to,reduce
R5856 T20668 T20665 advcl reduce,taken
R5857 T20669 T20670 amod endogenous,activity
R5858 T20670 T20668 dobj activity,reduce
R5859 T20671 T20672 compound β,galactosidase
R5860 T20672 T20670 compound galactosidase,activity
R5861 T20673 T20672 punct -,galactosidase
R5862 T20674 T20668 prep within,reduce
R5863 T20675 T20676 det the,kidney
R5864 T20676 T20674 pobj kidney,within
R5865 T20677 T20676 amod developing,kidney
R5866 T20678 T20668 prep by,reduce
R5867 T20679 T20678 pcomp increasing,by
R5868 T20680 T20679 dobj pH,increasing
R5869 T20681 T20680 prep of,pH
R5870 T20682 T20683 det the,solution
R5871 T20683 T20681 pobj solution,of
R5872 T20684 T20685 compound X,gal
R5873 T20685 T20687 compound gal,staining
R5874 T20686 T20685 punct -,gal
R5875 T20687 T20683 compound staining,solution
R5876 T20688 T20679 prep to,increasing
R5877 T20689 T20688 pobj 8.0,to
R5878 T20690 T20665 punct .,taken
R5879 T20692 T20693 nsubjpass Changes,quantitated
R5880 T20694 T20692 prep in,Changes
R5881 T20695 T20696 compound transgene,Gal
R5882 T20696 T20699 compound Gal,expression
R5883 T20697 T20696 compound β,Gal
R5884 T20698 T20696 punct -,Gal
R5885 T20699 T20694 pobj expression,in
R5886 T20700 T20693 auxpass were,quantitated
R5887 T20701 T20693 advcl using,quantitated
R5888 T20702 T20703 det a,kit
R5889 T20703 T20701 dobj kit,using
R5890 T20704 T20705 nmod β,Gal
R5891 T20705 T20703 nmod Gal,kit
R5892 T20706 T20705 punct -,Gal
R5893 T20707 T20703 nmod ELISA,kit
R5894 T20708 T20707 punct (,ELISA
R5895 T20709 T20710 npadvmod Enzyme,linked
R5896 T20710 T20712 amod linked,immunoassay
R5897 T20711 T20710 punct -,linked
R5898 T20712 T20707 appos immunoassay,ELISA
R5899 T20713 T20703 punct ),kit
R5900 T20714 T20715 punct (,Roche
R5901 T20715 T20703 parataxis Roche,kit
R5902 T20716 T20715 punct ", ",Roche
R5903 T20717 T20715 npadvmod Indianapolis,Roche
R5904 T20718 T20715 punct ", ",Roche
R5905 T20719 T20715 npadvmod IN,Roche
R5906 T20720 T20715 punct ),Roche
R5907 T20721 T20693 punct ", ",quantitated
R5908 T20722 T20693 advcl normalizing,quantitated
R5909 T20723 T20722 prep according,normalizing
R5910 T20724 T20723 prep to,according
R5911 T20725 T20726 amod total,protein
R5912 T20726 T20724 pobj protein,to
R5913 T20727 T20693 punct .,quantitated
R5914 T20729 T20730 det Each,genotype
R5915 T20730 T20731 nsubjpass genotype,represented
R5916 T20732 T20731 auxpass was,represented
R5917 T20733 T20731 agent by,represented
R5918 T20734 T20735 det a,size
R5919 T20735 T20733 pobj size,by
R5920 T20736 T20735 compound sample,size
R5921 T20737 T20735 prep of,size
R5922 T20738 T20737 pobj 4,of
R5923 T20739 T20731 prep except,represented
R5924 T20740 T20741 det the,groups
R5925 T20741 T20739 pobj groups,except
R5926 T20742 T20741 amod non-transgenic,groups
R5927 T20743 T20744 punct (,5
R5928 T20744 T20742 parataxis 5,non-transgenic
R5929 T20745 T20744 nsubj n,5
R5930 T20746 T20744 punct =,5
R5931 T20747 T20744 punct ),5
R5932 T20748 T20742 punct ", ",non-transgenic
R5933 T20749 T20742 conj Pygo1,non-transgenic
R5934 T20750 T20749 punct +,Pygo1
R5935 T20751 T20749 punct /,Pygo1
R5936 T20752 T20749 punct -,Pygo1
R5937 T20753 T20749 punct ;,Pygo1
R5938 T20754 T20749 appos Pygo2,Pygo1
R5939 T20755 T20754 punct +,Pygo2
R5940 T20756 T20754 punct /,Pygo2
R5941 T20757 T20754 punct +,Pygo2
R5942 T20758 T20759 punct (,6
R5943 T20759 T20749 parataxis 6,Pygo1
R5944 T20760 T20759 nsubj n,6
R5945 T20761 T20759 punct =,6
R5946 T20762 T20759 punct ),6
R5947 T20763 T20749 punct ", ",Pygo1
R5948 T20764 T20749 conj Pygo1,Pygo1
R5949 T20765 T20764 punct -,Pygo1
R5950 T20766 T20764 punct /,Pygo1
R5951 T20767 T20764 punct -,Pygo1
R5952 T20768 T20764 punct ;,Pygo1
R5953 T20769 T20764 appos Pygo2,Pygo1
R5954 T20770 T20769 punct +,Pygo2
R5955 T20771 T20769 punct /,Pygo2
R5956 T20772 T20769 punct -,Pygo2
R5957 T20773 T20774 punct (,8
R5958 T20774 T20764 parataxis 8,Pygo1
R5959 T20775 T20774 nsubj n,8
R5960 T20776 T20774 punct =,8
R5961 T20777 T20774 punct ),8
R5962 T20778 T20764 punct ", ",Pygo1
R5963 T20779 T20764 cc and,Pygo1
R5964 T20780 T20764 conj Pygo1,Pygo1
R5965 T20781 T20780 punct -,Pygo1
R5966 T20782 T20780 punct /,Pygo1
R5967 T20783 T20780 punct -,Pygo1
R5968 T20784 T20780 punct ;,Pygo1
R5969 T20785 T20780 appos Pygo2,Pygo1
R5970 T20786 T20785 punct -,Pygo2
R5971 T20787 T20785 punct /,Pygo2
R5972 T20788 T20785 punct -,Pygo2
R5973 T20789 T20790 punct (,6
R5974 T20790 T20780 parataxis 6,Pygo1
R5975 T20791 T20790 nsubj n,6
R5976 T20792 T20790 punct =,6
R5977 T20793 T20790 punct ),6
R5978 T20794 T20731 punct .,represented