PMC:1858683 / 13634-23616 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T9319	0-7	VBN	denotes	Reduced
T9321	8-17	JJ	denotes	canonical
T9322	18-21	NN	denotes	Wnt
T9320	22-31	NN	denotes	signaling
T9323	32-34	IN	denotes	in
T9324	35-40	NN	denotes	Pygo1
T9326	40-41	HYPH	denotes	/
T9325	41-46	NN	denotes	Pygo2
T9327	47-53	NN	denotes	mutant
T9328	54-58	NNS	denotes	mice
T9329	58-186	sentence	denotes	The BAT-gal transgene reporter of canonical Wnt signaling [21] was used to examine changes in Wnt signaling in the mutant mice.
T9330	59-62	DT	denotes	The
T9332	63-66	NN	denotes	BAT
T9334	66-67	HYPH	denotes	-
T9333	67-70	NN	denotes	gal
T9335	71-80	NN	denotes	transgene
T9331	81-89	NN	denotes	reporter
T9337	90-92	IN	denotes	of
T9338	93-102	JJ	denotes	canonical
T9340	103-106	NN	denotes	Wnt
T9339	107-116	NN	denotes	signaling
T9341	117-118	-LRB-	denotes	[
T9342	118-120	CD	denotes	21
T9343	120-121	-RRB-	denotes	]
T9344	122-125	VBD	denotes	was
T9336	126-130	VBN	denotes	used
T9345	131-133	TO	denotes	to
T9346	134-141	VB	denotes	examine
T9347	142-149	NNS	denotes	changes
T9348	150-152	IN	denotes	in
T9349	153-156	NN	denotes	Wnt
T9350	157-166	NN	denotes	signaling
T9351	167-169	IN	denotes	in
T9352	170-173	DT	denotes	the
T9354	174-180	NN	denotes	mutant
T9353	181-185	NNS	denotes	mice
T9355	185-186	.	denotes	.
T9356	186-299	sentence	denotes	Both Pygo2-/- and Pygo1/Pygo2 double-null E10.5 embryos showed a decrease in canonical Wnt signaling (Figure 5).
T9357	187-191	CC	denotes	Both
T9358	192-197	NN	denotes	Pygo2
T9360	197-198	SYM	denotes	-
T9361	198-199	HYPH	denotes	/
T9362	199-200	SYM	denotes	-
T9363	201-204	CC	denotes	and
T9364	205-210	NN	denotes	Pygo1
T9366	210-211	HYPH	denotes	/
T9365	211-216	NN	denotes	Pygo2
T9367	217-223	JJ	denotes	double
T9368	223-224	HYPH	denotes	-
T9359	224-228	JJ	denotes	null
T9370	229-234	NN	denotes	E10.5
T9369	235-242	NNS	denotes	embryos
T9371	243-249	VBD	denotes	showed
T9372	250-251	DT	denotes	a
T9373	252-260	NN	denotes	decrease
T9374	261-263	IN	denotes	in
T9375	264-273	JJ	denotes	canonical
T9377	274-277	NN	denotes	Wnt
T9376	278-287	NN	denotes	signaling
T9378	288-289	-LRB-	denotes	(
T9379	289-295	NN	denotes	Figure
T9380	296-297	CD	denotes	5
T9381	297-298	-RRB-	denotes	)
T9382	298-299	.	denotes	.
T9383	299-498	sentence	denotes	There was, however, tissue-specific variability in the degree of reduction, with the somites, for example, showing strong reduction, while the mutant telencephalon still showed robust Wnt signaling.
T9384	300-305	EX	denotes	There
T9385	306-309	VBD	denotes	was
T9386	309-311	,	denotes	,
T9387	311-318	RB	denotes	however
T9388	318-320	,	denotes	,
T9389	320-326	NN	denotes	tissue
T9391	326-327	HYPH	denotes	-
T9390	327-335	JJ	denotes	specific
T9392	336-347	NN	denotes	variability
T9393	348-350	IN	denotes	in
T9394	351-354	DT	denotes	the
T9395	355-361	NN	denotes	degree
T9396	362-364	IN	denotes	of
T9397	365-374	NN	denotes	reduction
T9398	374-376	,	denotes	,
T9399	376-380	IN	denotes	with
T9400	381-384	DT	denotes	the
T9401	385-392	NNS	denotes	somites
T9403	392-394	,	denotes	,
T9404	394-397	IN	denotes	for
T9405	398-405	NN	denotes	example
T9406	405-407	,	denotes	,
T9402	407-414	VBG	denotes	showing
T9407	415-421	JJ	denotes	strong
T9408	422-431	NN	denotes	reduction
T9409	431-433	,	denotes	,
T9410	433-438	IN	denotes	while
T9412	439-442	DT	denotes	the
T9414	443-449	NN	denotes	mutant
T9413	450-463	NN	denotes	telencephalon
T9415	464-469	RB	denotes	still
T9411	470-476	VBD	denotes	showed
T9416	477-483	JJ	denotes	robust
T9418	484-487	NN	denotes	Wnt
T9417	488-497	NN	denotes	signaling
T9419	497-498	.	denotes	.
T9420	498-648	sentence	denotes	These results suggest that the mammalian Pygo genes are significant modulators of canonical Wnt signaling in some, but not in all developing systems.
T9421	499-504	DT	denotes	These
T9422	505-512	NNS	denotes	results
T9423	513-520	VBP	denotes	suggest
T9424	521-525	IN	denotes	that
T9426	526-529	DT	denotes	the
T9428	530-539	JJ	denotes	mammalian
T9429	540-544	NN	denotes	Pygo
T9427	545-550	NNS	denotes	genes
T9425	551-554	VBP	denotes	are
T9430	555-566	JJ	denotes	significant
T9431	567-577	NNS	denotes	modulators
T9432	578-580	IN	denotes	of
T9433	581-590	JJ	denotes	canonical
T9435	591-594	NN	denotes	Wnt
T9434	595-604	NN	denotes	signaling
T9436	605-607	IN	denotes	in
T9437	608-612	DT	denotes	some
T9438	612-614	,	denotes	,
T9439	614-617	CC	denotes	but
T9440	618-621	RB	denotes	not
T9441	622-624	IN	denotes	in
T9442	625-628	DT	denotes	all
T9443	629-639	VBG	denotes	developing
T9444	640-647	NNS	denotes	systems
T9445	647-648	.	denotes	.
T9446	648-649	sentence	denotes
T24238	659-666	VBN	denotes	Reduced
T24240	667-676	JJ	denotes	canonical
T24241	677-680	NN	denotes	Wnt
T24239	681-690	NN	denotes	signaling
T24242	691-693	IN	denotes	in
T24243	694-699	NN	denotes	Pygo2
T24245	700-703	CC	denotes	and
T24246	704-709	NN	denotes	Pygo1
T24248	709-710	HYPH	denotes	/
T24247	710-715	NN	denotes	Pygo2
T24244	716-722	NN	denotes	mutant
T24249	723-730	NNS	denotes	embryos
T24250	730-731	.	denotes	.
T24251	731-814	sentence	denotes	E10.5 embryos, all with the BAT-gal transgene reporter of canonical Wnt signaling.
T24252	732-737	NN	denotes	E10.5
T24253	738-745	NNS	denotes	embryos
T24254	745-747	,	denotes	,
T24255	747-750	DT	denotes	all
T24256	751-755	IN	denotes	with
T24257	756-759	DT	denotes	the
T24259	760-763	NN	denotes	BAT
T24261	763-764	HYPH	denotes	-
T24260	764-767	NN	denotes	gal
T24262	768-777	NN	denotes	transgene
T24258	778-786	NN	denotes	reporter
T24263	787-789	IN	denotes	of
T24264	790-799	JJ	denotes	canonical
T24266	800-803	NN	denotes	Wnt
T24265	804-813	NN	denotes	signaling
T24267	813-814	.	denotes	.
T24268	814-1005	sentence	denotes	(A) Pygo1+/+/Pygo2+/- embryos showed normal X-gal staining in the developing brain, pharyngeal pouches, otic vesicle, apical ectodermal ridges of the fore and hind limb buds, and in somites.
T24269	815-816	-LRB-	denotes	(
T24270	816-817	LS	denotes	A
T24272	817-818	-RRB-	denotes	)
T24273	819-824	NN	denotes	Pygo1
T24275	824-825	SYM	denotes	+
T24276	825-826	HYPH	denotes	/
T24277	826-827	SYM	denotes	+
T24278	827-828	HYPH	denotes	/
T24279	828-833	NN	denotes	Pygo2
T24280	833-834	SYM	denotes	+
T24281	834-835	HYPH	denotes	/
T24282	835-836	SYM	denotes	-
T24274	837-844	NNS	denotes	embryos
T24271	845-851	VBD	denotes	showed
T24283	852-858	JJ	denotes	normal
T24285	859-860	NN	denotes	X
T24287	860-861	HYPH	denotes	-
T24286	861-864	NN	denotes	gal
T24284	865-873	NN	denotes	staining
T24288	874-876	IN	denotes	in
T24289	877-880	DT	denotes	the
T24291	881-891	VBG	denotes	developing
T24290	892-897	NN	denotes	brain
T24292	897-899	,	denotes	,
T24293	899-909	JJ	denotes	pharyngeal
T24294	910-917	NNS	denotes	pouches
T24295	917-919	,	denotes	,
T24296	919-923	JJ	denotes	otic
T24297	924-931	NN	denotes	vesicle
T24298	931-933	,	denotes	,
T24299	933-939	JJ	denotes	apical
T24301	940-950	JJ	denotes	ectodermal
T24300	951-957	NNS	denotes	ridges
T24302	958-960	IN	denotes	of
T24303	961-964	DT	denotes	the
T24305	965-969	JJ	denotes	fore
T24307	970-973	CC	denotes	and
T24308	974-978	JJ	denotes	hind
T24306	979-983	NN	denotes	limb
T24304	984-988	NNS	denotes	buds
T24309	988-990	,	denotes	,
T24310	990-993	CC	denotes	and
T24311	994-996	IN	denotes	in
T24312	997-1004	NNS	denotes	somites
T24313	1004-1005	.	denotes	.
T24314	1005-1215	sentence	denotes	(B) Pygo1+/-/Pygo2-/- embryos, with loss of both Pygo2 alleles, showed reduced but not absent BAT-gal reporter expression in many developing structures, including pharyngeal pouches, otic vesicle, and somites.
T24315	1006-1007	-LRB-	denotes	(
T24316	1007-1008	LS	denotes	B
T24318	1008-1009	-RRB-	denotes	)
T24319	1010-1015	NN	denotes	Pygo1
T24321	1015-1016	SYM	denotes	+
T24322	1016-1017	HYPH	denotes	/
T24323	1017-1018	SYM	denotes	-
T24324	1018-1019	HYPH	denotes	/
T24325	1019-1024	NN	denotes	Pygo2
T24326	1024-1025	SYM	denotes	-
T24327	1025-1026	HYPH	denotes	/
T24328	1026-1027	SYM	denotes	-
T24320	1028-1035	NNS	denotes	embryos
T24329	1035-1037	,	denotes	,
T24330	1037-1041	IN	denotes	with
T24331	1042-1046	NN	denotes	loss
T24332	1047-1049	IN	denotes	of
T24333	1050-1054	DT	denotes	both
T24335	1055-1060	NN	denotes	Pygo2
T24334	1061-1068	NNS	denotes	alleles
T24336	1068-1070	,	denotes	,
T24317	1070-1076	VBD	denotes	showed
T24337	1077-1084	JJ	denotes	reduced
T24339	1085-1088	CC	denotes	but
T24340	1089-1092	RB	denotes	not
T24341	1093-1099	JJ	denotes	absent
T24342	1100-1103	NN	denotes	BAT
T24344	1103-1104	HYPH	denotes	-
T24343	1104-1107	NN	denotes	gal
T24338	1108-1116	NN	denotes	reporter
T24345	1117-1127	NN	denotes	expression
T24346	1128-1130	IN	denotes	in
T24347	1131-1135	JJ	denotes	many
T24349	1136-1146	VBG	denotes	developing
T24348	1147-1157	NNS	denotes	structures
T24350	1157-1159	,	denotes	,
T24351	1159-1168	VBG	denotes	including
T24352	1169-1179	JJ	denotes	pharyngeal
T24353	1180-1187	NNS	denotes	pouches
T24354	1187-1189	,	denotes	,
T24355	1189-1193	JJ	denotes	otic
T24356	1194-1201	NN	denotes	vesicle
T24357	1201-1203	,	denotes	,
T24358	1203-1206	CC	denotes	and
T24359	1207-1214	NNS	denotes	somites
T24360	1214-1215	.	denotes	.
T24361	1215-1348	sentence	denotes	(C) Pygo1-/-/Pygo2+/- embryo, with mutation of both Pygo1 alleles, but one wild-type Pygo2 allele, showed normal BAT-gal expression.
T24362	1216-1217	-LRB-	denotes	(
T24363	1217-1218	LS	denotes	C
T24365	1218-1219	-RRB-	denotes	)
T24366	1220-1225	NN	denotes	Pygo1
T24368	1225-1226	SYM	denotes	-
T24369	1226-1227	HYPH	denotes	/
T24370	1227-1228	SYM	denotes	-
T24371	1228-1229	HYPH	denotes	/
T24372	1229-1234	NN	denotes	Pygo2
T24373	1234-1235	SYM	denotes	+
T24374	1235-1236	HYPH	denotes	/
T24375	1236-1237	SYM	denotes	-
T24367	1238-1244	NN	denotes	embryo
T24376	1244-1246	,	denotes	,
T24377	1246-1250	IN	denotes	with
T24378	1251-1259	NN	denotes	mutation
T24379	1260-1262	IN	denotes	of
T24380	1263-1267	DT	denotes	both
T24382	1268-1273	NN	denotes	Pygo1
T24381	1274-1281	NNS	denotes	alleles
T24383	1281-1283	,	denotes	,
T24384	1283-1286	CC	denotes	but
T24385	1287-1290	CD	denotes	one
T24387	1291-1295	JJ	denotes	wild
T24389	1295-1296	HYPH	denotes	-
T24388	1296-1300	NN	denotes	type
T24390	1301-1306	NN	denotes	Pygo2
T24386	1307-1313	NN	denotes	allele
T24391	1313-1315	,	denotes	,
T24364	1315-1321	VBD	denotes	showed
T24392	1322-1328	JJ	denotes	normal
T24394	1329-1332	NN	denotes	BAT
T24396	1332-1333	HYPH	denotes	-
T24395	1333-1336	NN	denotes	gal
T24393	1337-1347	NN	denotes	expression
T24397	1347-1348	.	denotes	.
T24398	1348-1496	sentence	denotes	(D) Double-homozygous mutant Pygo1-/-/Pygo2-/- embryos still showed some remaining BAT-gal expression, suggesting residual canonical Wnt signaling.
T24399	1349-1350	-LRB-	denotes	(
T24400	1350-1351	LS	denotes	D
T24402	1351-1352	-RRB-	denotes	)
T24403	1353-1359	JJ	denotes	Double
T24405	1359-1360	HYPH	denotes	-
T24404	1360-1370	JJ	denotes	homozygous
T24407	1371-1377	NN	denotes	mutant
T24408	1378-1383	JJ	denotes	Pygo1
T24409	1383-1384	SYM	denotes	-
T24410	1384-1385	HYPH	denotes	/
T24411	1385-1386	SYM	denotes	-
T24412	1386-1387	HYPH	denotes	/
T24413	1387-1392	NN	denotes	Pygo2
T24414	1392-1393	SYM	denotes	-
T24415	1393-1394	HYPH	denotes	/
T24416	1394-1395	SYM	denotes	-
T24406	1396-1403	NNS	denotes	embryos
T24417	1404-1409	RB	denotes	still
T24401	1410-1416	VBD	denotes	showed
T24418	1417-1421	DT	denotes	some
T24420	1422-1431	VBG	denotes	remaining
T24421	1432-1435	NN	denotes	BAT
T24423	1435-1436	HYPH	denotes	-
T24422	1436-1439	NN	denotes	gal
T24419	1440-1450	NN	denotes	expression
T24424	1450-1452	,	denotes	,
T24425	1452-1462	VBG	denotes	suggesting
T24426	1463-1471	JJ	denotes	residual
T24428	1472-1481	JJ	denotes	canonical
T24429	1482-1485	NN	denotes	Wnt
T24427	1486-1495	NN	denotes	signaling
T24430	1495-1496	.	denotes	.
T24431	1496-1719	sentence	denotes	Embryos in panels (A) and (B) were from the same litter and were processed in parallel, while embryos in (C) and (D) were from a separate litter, also processed in parallel, and were slightly more developmentally advanced.
T24432	1497-1504	NNS	denotes	Embryos
T24434	1505-1507	IN	denotes	in
T24435	1508-1514	NNS	denotes	panels
T24437	1515-1516	-LRB-	denotes	(
T24436	1516-1517	NN	denotes	A
T24438	1517-1518	-RRB-	denotes	)
T24439	1519-1522	CC	denotes	and
T24440	1523-1524	-LRB-	denotes	(
T24441	1524-1525	NN	denotes	B
T24442	1525-1526	-RRB-	denotes	)
T24433	1527-1531	VBD	denotes	were
T24443	1532-1536	IN	denotes	from
T24444	1537-1540	DT	denotes	the
T24446	1541-1545	JJ	denotes	same
T24445	1546-1552	NN	denotes	litter
T24447	1553-1556	CC	denotes	and
T24448	1557-1561	VBD	denotes	were
T24449	1562-1571	VBN	denotes	processed
T24450	1572-1574	IN	denotes	in
T24451	1575-1583	NN	denotes	parallel
T24452	1583-1585	,	denotes	,
T24453	1585-1590	IN	denotes	while
T24455	1591-1598	NNS	denotes	embryos
T24456	1599-1601	IN	denotes	in
T24457	1602-1603	-LRB-	denotes	(
T24458	1603-1604	NN	denotes	C
T24459	1604-1605	-RRB-	denotes	)
T24460	1606-1609	CC	denotes	and
T24461	1610-1611	-LRB-	denotes	(
T24462	1611-1612	NN	denotes	D
T24463	1612-1613	-RRB-	denotes	)
T24454	1614-1618	VBD	denotes	were
T24464	1619-1623	IN	denotes	from
T24465	1624-1625	DT	denotes	a
T24467	1626-1634	JJ	denotes	separate
T24466	1635-1641	NN	denotes	litter
T24468	1641-1643	,	denotes	,
T24469	1643-1647	RB	denotes	also
T24470	1648-1657	VBN	denotes	processed
T24471	1658-1660	IN	denotes	in
T24472	1661-1669	NN	denotes	parallel
T24473	1669-1671	,	denotes	,
T24474	1671-1674	CC	denotes	and
T24475	1675-1679	VBD	denotes	were
T24476	1680-1688	RB	denotes	slightly
T24477	1689-1693	RBR	denotes	more
T24479	1694-1709	RB	denotes	developmentally
T24478	1710-1718	JJ	denotes	advanced
T24480	1718-1719	.	denotes	.
T24481	1719-1768	sentence	denotes	Original magnification (A, B) × 20; (C, D) × 16.
T24482	1720-1728	JJ	denotes	Original
T24483	1729-1742	NN	denotes	magnification
T24485	1743-1744	-LRB-	denotes	(
T24487	1744-1745	NN	denotes	A
T24488	1745-1747	,	denotes	,
T24486	1747-1748	NN	denotes	B
T24489	1748-1749	-RRB-	denotes	)
T24490	1750-1751	SYM	denotes	×
T24484	1752-1754	CD	denotes	20
T24491	1754-1755	:	denotes	;
T24492	1756-1757	-LRB-	denotes	(
T24494	1757-1758	NN	denotes	C
T24495	1758-1760	,	denotes	,
T24493	1760-1761	NN	denotes	D
T24497	1761-1762	-RRB-	denotes	)
T24498	1763-1764	SYM	denotes	×
T24496	1765-1767	CD	denotes	16
T24499	1767-1768	.	denotes	.
T9448	1769-1770	NN	denotes	W
T9447	1769-1771	sentence	denotes	We
T9449	1770-1771	NNS	denotes	e
T9450	1771-1777	sentence	denotes	also
T9451	1772-1774	DT	denotes	al
T9453	1774-1775	NN	denotes	s
T9452	1775-1776	NN	denotes	o
T9455	1777-1778	NNS	denotes	e
T9454	1777-1778	sentence	denotes	e
T9457	1778-1779	NN	denotes	x
T9456	1778-1782	sentence	denotes	xami
T9458	1779-1780	JJ	denotes	a
T9459	1780-1782	NNS	denotes	mi
T9461	1782-1783	NN	denotes	n
T9460	1782-1796	sentence	denotes	ned BAT-gal re
T9462	1783-1784	NNS	denotes	e
T9463	1784-1785	JJ	denotes	d
T9465	1786-1789	NN	denotes	BAT
T9467	1789-1790	HYPH	denotes	-
T9466	1790-1793	NN	denotes	gal
T9464	1794-1796	NN	denotes	re
T9469	1796-1798	NN	denotes	po
T9468	1796-1800	sentence	denotes	port
T9470	1798-1799	JJ	denotes	r
T9471	1799-1800	NN	denotes	t
T9474	1800-1801	NNS	denotes	e
T9472	1800-1804	sentence	denotes	er e
T9473	1801-1802	VBD	denotes	r
T9475	1803-1804	DT	denotes	e
T9477	1804-1805	VBD	denotes	x
T9476	1804-1881	sentence	denotes	xpression in more detail in the developing urogenital system of Pygo mutants.
T9478	1805-1808	NN	denotes	pre
T9479	1808-1813	NN	denotes	ssion
T9480	1814-1816	IN	denotes	in
T9481	1817-1821	JJR	denotes	more
T9482	1822-1828	NN	denotes	detail
T9483	1829-1831	IN	denotes	in
T9484	1832-1835	DT	denotes	the
T9486	1836-1846	VBG	denotes	developing
T9487	1847-1857	JJ	denotes	urogenital
T9485	1858-1864	NN	denotes	system
T9488	1865-1867	IN	denotes	of
T9489	1868-1872	NN	denotes	Pygo
T9490	1873-1880	NNS	denotes	mutants
T9491	1880-1881	.	denotes	.
T9492	1881-1984	sentence	denotes	The results suggested that the Pygo2 gene is required for canonical Wnt signaling in the nephric duct.
T9493	1882-1885	DT	denotes	The
T9494	1886-1893	NNS	denotes	results
T9495	1894-1903	VBD	denotes	suggested
T9496	1904-1908	IN	denotes	that
T9498	1909-1912	DT	denotes	the
T9500	1913-1918	NN	denotes	Pygo2
T9499	1919-1923	NN	denotes	gene
T9501	1924-1926	VBZ	denotes	is
T9497	1927-1935	VBN	denotes	required
T9502	1936-1939	IN	denotes	for
T9503	1940-1949	JJ	denotes	canonical
T9505	1950-1953	NN	denotes	Wnt
T9504	1954-1963	NN	denotes	signaling
T9506	1964-1966	IN	denotes	in
T9507	1967-1970	DT	denotes	the
T9509	1971-1978	JJ	denotes	nephric
T9508	1979-1983	NN	denotes	duct
T9510	1983-1984	.	denotes	.
T9511	1984-2170	sentence	denotes	Both Pygo2 null and Pygo1/Pygo2 double-null E10.5 embryos showed an absence of reporter expression in the nephric duct, while control littermates showed strong expression (Figure 6A–C).
T9512	1985-1989	CC	denotes	Both
T9514	1990-1995	NN	denotes	Pygo2
T9513	1996-2000	JJ	denotes	null
T9516	2001-2004	CC	denotes	and
T9517	2005-2010	NN	denotes	Pygo1
T9519	2010-2011	HYPH	denotes	/
T9518	2011-2016	NN	denotes	Pygo2
T9521	2017-2023	JJ	denotes	double
T9522	2023-2024	HYPH	denotes	-
T9520	2024-2028	JJ	denotes	null
T9523	2029-2034	NN	denotes	E10.5
T9515	2035-2042	NNS	denotes	embryos
T9524	2043-2049	VBD	denotes	showed
T9525	2050-2052	DT	denotes	an
T9526	2053-2060	NN	denotes	absence
T9527	2061-2063	IN	denotes	of
T9528	2064-2072	NN	denotes	reporter
T9529	2073-2083	NN	denotes	expression
T9530	2084-2086	IN	denotes	in
T9531	2087-2090	DT	denotes	the
T9533	2091-2098	JJ	denotes	nephric
T9532	2099-2103	NN	denotes	duct
T9534	2103-2105	,	denotes	,
T9535	2105-2110	IN	denotes	while
T9537	2111-2118	NN	denotes	control
T9538	2119-2130	NNS	denotes	littermates
T9536	2131-2137	VBD	denotes	showed
T9539	2138-2144	JJ	denotes	strong
T9540	2145-2155	NN	denotes	expression
T9541	2156-2157	-LRB-	denotes	(
T9543	2157-2163	NN	denotes	Figure
T9542	2164-2166	NN	denotes	6A
T9544	2166-2167	SYM	denotes	–
T9545	2167-2168	NN	denotes	C
T9546	2168-2169	-RRB-	denotes	)
T9547	2169-2170	.	denotes	.
T9548	2170-2366	sentence	denotes	In Pygo1/Pygo2 double-null mutants, the nephric duct did form, however, and give rise to the ureteric bud outgrowth, which showed reduced but not absent BAT-gal reporter expression (Figure 6A–C).
T9549	2171-2173	IN	denotes	In
T9551	2174-2179	NN	denotes	Pygo1
T9553	2179-2180	HYPH	denotes	/
T9552	2180-2185	NN	denotes	Pygo2
T9555	2186-2192	JJ	denotes	double
T9556	2192-2193	HYPH	denotes	-
T9554	2193-2197	JJ	denotes	null
T9557	2198-2205	NNS	denotes	mutants
T9558	2205-2207	,	denotes	,
T9559	2207-2210	DT	denotes	the
T9561	2211-2218	JJ	denotes	nephric
T9560	2219-2223	NN	denotes	duct
T9562	2224-2227	VBD	denotes	did
T9550	2228-2232	VB	denotes	form
T9563	2232-2234	,	denotes	,
T9564	2234-2241	RB	denotes	however
T9565	2241-2243	,	denotes	,
T9566	2243-2246	CC	denotes	and
T9567	2247-2251	VB	denotes	give
T9568	2252-2256	NN	denotes	rise
T9569	2257-2259	IN	denotes	to
T9570	2260-2263	DT	denotes	the
T9572	2264-2272	JJ	denotes	ureteric
T9573	2273-2276	NN	denotes	bud
T9571	2277-2286	NN	denotes	outgrowth
T9574	2286-2288	,	denotes	,
T9575	2288-2293	WDT	denotes	which
T9576	2294-2300	VBD	denotes	showed
T9577	2301-2308	VBN	denotes	reduced
T9579	2309-2312	CC	denotes	but
T9580	2313-2316	RB	denotes	not
T9581	2317-2323	JJ	denotes	absent
T9582	2324-2327	NN	denotes	BAT
T9584	2327-2328	HYPH	denotes	-
T9583	2328-2331	NN	denotes	gal
T9585	2332-2340	NN	denotes	reporter
T9578	2341-2351	NN	denotes	expression
T9586	2352-2353	-LRB-	denotes	(
T9588	2353-2359	NN	denotes	Figure
T9587	2360-2362	NN	denotes	6A
T9589	2362-2363	SYM	denotes	–
T9590	2363-2364	NN	denotes	C
T9591	2364-2365	-RRB-	denotes	)
T9592	2365-2366	.	denotes	.
T9593	2366-2367	sentence	denotes
T25934	2377-2382	NN	denotes	Pygo2
T25936	2383-2385	VBZ	denotes	is
T25935	2386-2394	VBN	denotes	required
T25937	2395-2398	IN	denotes	for
T25938	2399-2402	NN	denotes	BAT
T25940	2402-2403	HYPH	denotes	-
T25939	2403-2406	NN	denotes	gal
T25941	2407-2415	NN	denotes	reporter
T25942	2416-2426	NN	denotes	expression
T25943	2427-2429	IN	denotes	in
T25944	2430-2438	JJ	denotes	ureteric
T25945	2439-2442	NN	denotes	bud
T25947	2442-2443	HYPH	denotes	-
T25946	2443-2450	VBN	denotes	derived
T25948	2451-2461	NNS	denotes	structures
T25949	2462-2464	IN	denotes	of
T25950	2465-2468	DT	denotes	the
T25952	2469-2479	VBG	denotes	developing
T25951	2480-2486	NN	denotes	kidney
T25953	2486-2487	.	denotes	.
T25954	2487-2597	sentence	denotes	X-Gal staining of BAT-gal transgenic (A-C) E10.5, and (E-L) E13.5 urogenital tracts, and (M-O) E18.5 kidneys.
T25955	2488-2489	NN	denotes	X
T25957	2489-2490	HYPH	denotes	-
T25956	2490-2493	NN	denotes	Gal
T25958	2494-2502	NN	denotes	staining
T25959	2503-2505	IN	denotes	of
T25960	2506-2509	NN	denotes	BAT
T25962	2509-2510	HYPH	denotes	-
T25961	2510-2513	NN	denotes	gal
T25963	2514-2524	JJ	denotes	transgenic
T25965	2525-2526	-LRB-	denotes	(
T25966	2526-2527	NN	denotes	A
T25968	2527-2528	SYM	denotes	-
T25969	2528-2529	NN	denotes	C
T25970	2529-2530	-RRB-	denotes	)
T25967	2531-2536	NN	denotes	E10.5
T25971	2536-2538	,	denotes	,
T25972	2538-2541	CC	denotes	and
T25973	2542-2543	-LRB-	denotes	(
T25974	2543-2544	NN	denotes	E
T25976	2544-2545	SYM	denotes	-
T25977	2545-2546	NN	denotes	L
T25978	2546-2547	-RRB-	denotes	)
T25975	2548-2553	NN	denotes	E13.5
T25979	2554-2564	JJ	denotes	urogenital
T25964	2565-2571	NNS	denotes	tracts
T25980	2571-2573	,	denotes	,
T25981	2573-2576	CC	denotes	and
T25982	2577-2578	-LRB-	denotes	(
T25983	2578-2579	NN	denotes	M
T25985	2579-2580	SYM	denotes	-
T25986	2580-2581	NN	denotes	O
T25987	2581-2582	-RRB-	denotes	)
T25988	2583-2588	NN	denotes	E18.5
T25984	2589-2596	NNS	denotes	kidneys
T25989	2596-2597	.	denotes	.
T25990	2597-2657	sentence	denotes	(A) Pygo1-/-/Pygo2+/+ (left) and Pygo1+/-/Pygo2-/- (right).
T25991	2598-2599	-LRB-	denotes	(
T25992	2599-2600	LS	denotes	A
T25994	2600-2601	-RRB-	denotes	)
T25993	2602-2607	NN	denotes	Pygo1
T25995	2607-2608	SYM	denotes	-
T25996	2608-2609	HYPH	denotes	/
T25997	2609-2610	SYM	denotes	-
T25998	2610-2611	HYPH	denotes	/
T25999	2611-2616	NN	denotes	Pygo2
T26000	2616-2617	SYM	denotes	+
T26001	2617-2618	HYPH	denotes	/
T26002	2618-2619	SYM	denotes	+
T26003	2620-2621	-LRB-	denotes	(
T26004	2621-2625	JJ	denotes	left
T26005	2625-2626	-RRB-	denotes	)
T26006	2627-2630	CC	denotes	and
T26007	2631-2636	NN	denotes	Pygo1
T26008	2636-2637	SYM	denotes	+
T26009	2637-2638	HYPH	denotes	/
T26010	2638-2639	SYM	denotes	-
T26011	2639-2640	HYPH	denotes	/
T26012	2640-2645	NN	denotes	Pygo2
T26013	2645-2646	SYM	denotes	-
T26014	2646-2647	HYPH	denotes	/
T26015	2647-2648	SYM	denotes	-
T26016	2649-2650	-LRB-	denotes	(
T26017	2650-2655	JJ	denotes	right
T26018	2655-2656	-RRB-	denotes	)
T26019	2656-2657	.	denotes	.
T26020	2657-2824	sentence	denotes	Note the loss of reporter activity in the nephric duct (black arrowhead) and reduction of staining in the ureteric bud (white arrow) of the Pygo2 null embryo (Right).
T26021	2658-2662	VB	denotes	Note
T26022	2663-2666	DT	denotes	the
T26023	2667-2671	NN	denotes	loss
T26024	2672-2674	IN	denotes	of
T26025	2675-2683	NN	denotes	reporter
T26026	2684-2692	NN	denotes	activity
T26027	2693-2695	IN	denotes	in
T26028	2696-2699	DT	denotes	the
T26030	2700-2707	JJ	denotes	nephric
T26029	2708-2712	NN	denotes	duct
T26031	2713-2714	-LRB-	denotes	(
T26033	2714-2719	JJ	denotes	black
T26032	2720-2729	NN	denotes	arrowhead
T26034	2729-2730	-RRB-	denotes	)
T26035	2731-2734	CC	denotes	and
T26036	2735-2744	NN	denotes	reduction
T26037	2745-2747	IN	denotes	of
T26038	2748-2756	NN	denotes	staining
T26039	2757-2759	IN	denotes	in
T26040	2760-2763	DT	denotes	the
T26042	2764-2772	JJ	denotes	ureteric
T26041	2773-2776	NN	denotes	bud
T26043	2777-2778	-LRB-	denotes	(
T26045	2778-2783	JJ	denotes	white
T26044	2784-2789	NN	denotes	arrow
T26046	2789-2790	-RRB-	denotes	)
T26047	2791-2793	IN	denotes	of
T26048	2794-2797	DT	denotes	the
T26050	2798-2803	NN	denotes	Pygo2
T26051	2804-2808	JJ	denotes	null
T26049	2809-2815	NN	denotes	embryo
T26052	2816-2817	-LRB-	denotes	(
T26053	2817-2822	JJ	denotes	Right
T26054	2822-2823	-RRB-	denotes	)
T26055	2823-2824	.	denotes	.
T26056	2824-2960	sentence	denotes	(B) Pygo1-/-/Pygo2+/- E10.5 embryo with BAT-gal reporter activity in the nephric duct (black arrowhead) and ureteric bud (white arrow).
T26057	2825-2826	-LRB-	denotes	(
T26058	2826-2827	LS	denotes	B
T26060	2827-2828	-RRB-	denotes	)
T26061	2829-2834	NN	denotes	Pygo1
T26062	2834-2835	SYM	denotes	-
T26063	2835-2836	HYPH	denotes	/
T26064	2836-2837	SYM	denotes	-
T26065	2837-2838	HYPH	denotes	/
T26066	2838-2843	NN	denotes	Pygo2
T26067	2843-2844	SYM	denotes	+
T26068	2844-2845	HYPH	denotes	/
T26069	2845-2846	SYM	denotes	-
T26070	2847-2852	NN	denotes	E10.5
T26059	2853-2859	NN	denotes	embryo
T26071	2860-2864	IN	denotes	with
T26072	2865-2868	NN	denotes	BAT
T26074	2868-2869	HYPH	denotes	-
T26073	2869-2872	NN	denotes	gal
T26075	2873-2881	NN	denotes	reporter
T26076	2882-2890	NN	denotes	activity
T26077	2891-2893	IN	denotes	in
T26078	2894-2897	DT	denotes	the
T26080	2898-2905	JJ	denotes	nephric
T26079	2906-2910	NN	denotes	duct
T26081	2911-2912	-LRB-	denotes	(
T26083	2912-2917	JJ	denotes	black
T26082	2918-2927	NN	denotes	arrowhead
T26084	2927-2928	-RRB-	denotes	)
T26085	2929-2932	CC	denotes	and
T26086	2933-2941	JJ	denotes	ureteric
T26087	2942-2945	NN	denotes	bud
T26088	2946-2947	-LRB-	denotes	(
T26090	2947-2952	JJ	denotes	white
T26089	2953-2958	NN	denotes	arrow
T26091	2958-2959	-RRB-	denotes	)
T26092	2959-2960	.	denotes	.
T26093	2960-3086	sentence	denotes	(C) Pygo1-/-/Pygo-/- embryo, with reporter expression lost in the nephric duct and reduced in the ureteric bud (white arrow).
T26094	2961-2962	-LRB-	denotes	(
T26095	2962-2963	LS	denotes	C
T26097	2963-2964	-RRB-	denotes	)
T26098	2965-2970	NN	denotes	Pygo1
T26099	2970-2971	SYM	denotes	-
T26100	2971-2972	HYPH	denotes	/
T26101	2972-2973	SYM	denotes	-
T26102	2973-2974	HYPH	denotes	/
T26103	2974-2978	NN	denotes	Pygo
T26104	2978-2979	SYM	denotes	-
T26105	2979-2980	HYPH	denotes	/
T26106	2980-2981	SYM	denotes	-
T26096	2982-2988	NN	denotes	embryo
T26107	2988-2990	,	denotes	,
T26108	2990-2994	IN	denotes	with
T26110	2995-3003	NN	denotes	reporter
T26111	3004-3014	NN	denotes	expression
T26109	3015-3019	JJ	denotes	lost
T26112	3020-3022	IN	denotes	in
T26113	3023-3026	DT	denotes	the
T26115	3027-3034	JJ	denotes	nephric
T26114	3035-3039	NN	denotes	duct
T26116	3040-3043	CC	denotes	and
T26117	3044-3051	VBN	denotes	reduced
T26118	3052-3054	IN	denotes	in
T26119	3055-3058	DT	denotes	the
T26121	3059-3067	JJ	denotes	ureteric
T26120	3068-3071	NN	denotes	bud
T26122	3072-3073	-LRB-	denotes	(
T26124	3073-3078	JJ	denotes	white
T26123	3079-3084	NN	denotes	arrow
T26125	3084-3085	-RRB-	denotes	)
T26126	3085-3086	.	denotes	.
T26127	3086-3266	sentence	denotes	(D) Control background X-gal staining of a urogenital tract from an E13.5 embryo without the BAT-gal transgene (Non-Tg), showing absence of endogenous beta-galactosidase activity.
T26128	3087-3088	-LRB-	denotes	(
T26129	3088-3089	LS	denotes	D
T26131	3089-3090	-RRB-	denotes	)
T26132	3091-3098	NN	denotes	Control
T26133	3099-3109	NN	denotes	background
T26134	3110-3111	NN	denotes	X
T26136	3111-3112	HYPH	denotes	-
T26135	3112-3115	NN	denotes	gal
T26130	3116-3124	NN	denotes	staining
T26137	3125-3127	IN	denotes	of
T26138	3128-3129	DT	denotes	a
T26140	3130-3140	JJ	denotes	urogenital
T26139	3141-3146	NN	denotes	tract
T26141	3147-3151	IN	denotes	from
T26142	3152-3154	DT	denotes	an
T26144	3155-3160	NN	denotes	E13.5
T26143	3161-3167	NN	denotes	embryo
T26145	3168-3175	IN	denotes	without
T26146	3176-3179	DT	denotes	the
T26148	3180-3183	NN	denotes	BAT
T26150	3183-3184	HYPH	denotes	-
T26149	3184-3187	NN	denotes	gal
T26147	3188-3197	NN	denotes	transgene
T26151	3198-3199	-LRB-	denotes	(
T26152	3199-3205	NN	denotes	Non-Tg
T26153	3205-3206	-RRB-	denotes	)
T26154	3206-3208	,	denotes	,
T26155	3208-3215	VBG	denotes	showing
T26156	3216-3223	NN	denotes	absence
T26157	3224-3226	IN	denotes	of
T26158	3227-3237	JJ	denotes	endogenous
T26160	3238-3242	NN	denotes	beta
T26162	3242-3243	HYPH	denotes	-
T26161	3243-3256	NN	denotes	galactosidase
T26159	3257-3265	NN	denotes	activity
T26163	3265-3266	.	denotes	.
T26164	3266-3430	sentence	denotes	(E) Pygo1+/-/Pygo2+/+, with BAT-gal reporter activity in the ureteric compartment of the developing kidney, including the ureteric tips, ureteric tree, and ureter.
T26165	3267-3268	-LRB-	denotes	(
T26166	3268-3269	LS	denotes	E
T26168	3269-3270	-RRB-	denotes	)
T26167	3271-3276	NN	denotes	Pygo1
T26169	3276-3277	SYM	denotes	+
T26170	3277-3278	HYPH	denotes	/
T26171	3278-3279	SYM	denotes	-
T26172	3279-3280	HYPH	denotes	/
T26173	3280-3285	NN	denotes	Pygo2
T26174	3285-3286	SYM	denotes	+
T26175	3286-3287	HYPH	denotes	/
T26176	3287-3288	SYM	denotes	+
T26177	3288-3290	,	denotes	,
T26178	3290-3294	IN	denotes	with
T26179	3295-3298	NN	denotes	BAT
T26181	3298-3299	HYPH	denotes	-
T26180	3299-3302	NN	denotes	gal
T26182	3303-3311	NN	denotes	reporter
T26183	3312-3320	NN	denotes	activity
T26184	3321-3323	IN	denotes	in
T26185	3324-3327	DT	denotes	the
T26187	3328-3336	JJ	denotes	ureteric
T26186	3337-3348	NN	denotes	compartment
T26188	3349-3351	IN	denotes	of
T26189	3352-3355	DT	denotes	the
T26191	3356-3366	VBG	denotes	developing
T26190	3367-3373	NN	denotes	kidney
T26192	3373-3375	,	denotes	,
T26193	3375-3384	VBG	denotes	including
T26194	3385-3388	DT	denotes	the
T26196	3389-3397	JJ	denotes	ureteric
T26195	3398-3402	NNS	denotes	tips
T26197	3402-3404	,	denotes	,
T26198	3404-3412	JJ	denotes	ureteric
T26199	3413-3417	NN	denotes	tree
T26200	3417-3419	,	denotes	,
T26201	3419-3422	CC	denotes	and
T26202	3423-3429	NN	denotes	ureter
T26203	3429-3430	.	denotes	.
T26204	3430-3533	sentence	denotes	(F) Pygo1+/-/Pygo2-/-, with marked reduction of BAT-gal reporter activity in the ureteric compartment.
T26205	3431-3432	-LRB-	denotes	(
T26206	3432-3433	LS	denotes	F
T26208	3433-3434	-RRB-	denotes	)
T26207	3435-3440	NN	denotes	Pygo1
T26209	3440-3441	SYM	denotes	+
T26210	3441-3442	HYPH	denotes	/
T26211	3442-3443	SYM	denotes	-
T26212	3443-3444	HYPH	denotes	/
T26213	3444-3449	NN	denotes	Pygo2
T26214	3449-3450	SYM	denotes	-
T26215	3450-3451	HYPH	denotes	/
T26216	3451-3452	SYM	denotes	-
T26217	3452-3454	,	denotes	,
T26218	3454-3458	IN	denotes	with
T26219	3459-3465	JJ	denotes	marked
T26220	3466-3475	NN	denotes	reduction
T26221	3476-3478	IN	denotes	of
T26222	3479-3482	NN	denotes	BAT
T26224	3482-3483	HYPH	denotes	-
T26223	3483-3486	NN	denotes	gal
T26225	3487-3495	NN	denotes	reporter
T26226	3496-3504	NN	denotes	activity
T26227	3505-3507	IN	denotes	in
T26228	3508-3511	DT	denotes	the
T26230	3512-3520	JJ	denotes	ureteric
T26229	3521-3532	NN	denotes	compartment
T26231	3532-3533	.	denotes	.
T26232	3533-3646	sentence	denotes	(G) Pygo1+/-/Pygo2+/-, with reporter expression in the paramesonephric duct (white arrowhead) and ureteric tree.
T26233	3534-3535	-LRB-	denotes	(
T26234	3535-3536	LS	denotes	G
T26236	3536-3537	-RRB-	denotes	)
T26235	3538-3543	NN	denotes	Pygo1
T26237	3543-3544	SYM	denotes	+
T26238	3544-3545	HYPH	denotes	/
T26239	3545-3546	SYM	denotes	-
T26240	3546-3547	HYPH	denotes	/
T26241	3547-3552	NN	denotes	Pygo2
T26242	3552-3553	SYM	denotes	+
T26243	3553-3554	HYPH	denotes	/
T26244	3554-3555	SYM	denotes	-
T26245	3555-3557	,	denotes	,
T26246	3557-3561	IN	denotes	with
T26247	3562-3570	NN	denotes	reporter
T26248	3571-3581	NN	denotes	expression
T26249	3582-3584	IN	denotes	in
T26250	3585-3588	DT	denotes	the
T26252	3589-3604	JJ	denotes	paramesonephric
T26251	3605-3609	NN	denotes	duct
T26253	3610-3611	-LRB-	denotes	(
T26255	3611-3616	JJ	denotes	white
T26254	3617-3626	NN	denotes	arrowhead
T26256	3626-3627	-RRB-	denotes	)
T26257	3628-3631	CC	denotes	and
T26258	3632-3640	JJ	denotes	ureteric
T26259	3641-3645	NN	denotes	tree
T26260	3645-3646	.	denotes	.
T26261	3646-3837	sentence	denotes	(H, I) Pygo1-/-/Pygo2+/-, with (H) ventral view showing reporter expression in the paramesonephric duct (white arrowhead), and (I) dorsal view showing ureteric tree expression in the kidney.
T26262	3647-3648	-LRB-	denotes	(
T26263	3648-3649	LS	denotes	H
T26265	3649-3651	,	denotes	,
T26266	3651-3652	LS	denotes	I
T26267	3652-3653	-RRB-	denotes	)
T26264	3654-3659	NN	denotes	Pygo1
T26268	3659-3660	SYM	denotes	-
T26269	3660-3661	HYPH	denotes	/
T26270	3661-3662	SYM	denotes	-
T26271	3662-3663	HYPH	denotes	/
T26272	3663-3668	NN	denotes	Pygo2
T26273	3668-3669	SYM	denotes	+
T26274	3669-3670	HYPH	denotes	/
T26275	3670-3671	SYM	denotes	-
T26276	3671-3673	,	denotes	,
T26277	3673-3677	IN	denotes	with
T26278	3678-3679	-LRB-	denotes	(
T26279	3679-3680	LS	denotes	H
T26281	3680-3681	-RRB-	denotes	)
T26282	3682-3689	JJ	denotes	ventral
T26280	3690-3694	NN	denotes	view
T26283	3695-3702	VBG	denotes	showing
T26284	3703-3711	NN	denotes	reporter
T26285	3712-3722	NN	denotes	expression
T26286	3723-3725	IN	denotes	in
T26287	3726-3729	DT	denotes	the
T26289	3730-3745	JJ	denotes	paramesonephric
T26288	3746-3750	NN	denotes	duct
T26290	3751-3752	-LRB-	denotes	(
T26292	3752-3757	JJ	denotes	white
T26291	3758-3767	NN	denotes	arrowhead
T26293	3767-3768	-RRB-	denotes	)
T26294	3768-3770	,	denotes	,
T26295	3770-3773	CC	denotes	and
T26296	3774-3775	-LRB-	denotes	(
T26297	3775-3776	LS	denotes	I
T26299	3776-3777	-RRB-	denotes	)
T26300	3778-3784	JJ	denotes	dorsal
T26298	3785-3789	NN	denotes	view
T26301	3790-3797	VBG	denotes	showing
T26302	3798-3806	JJ	denotes	ureteric
T26303	3807-3811	NN	denotes	tree
T26304	3812-3822	NN	denotes	expression
T26305	3823-3825	IN	denotes	in
T26306	3826-3829	DT	denotes	the
T26307	3830-3836	NN	denotes	kidney
T26308	3836-3837	.	denotes	.
T26309	3837-3968	sentence	denotes	(J)Pygo1+/-/Pygo2+/-, a control processed in parallel with (K) and (L), with expression in ureteric tree and paramesonephric duct.
T26310	3838-3839	-LRB-	denotes	(
T26311	3839-3840	LS	denotes	J
T26313	3840-3841	-RRB-	denotes	)
T26312	3841-3846	NN	denotes	Pygo1
T26314	3846-3847	SYM	denotes	+
T26315	3847-3848	HYPH	denotes	/
T26316	3848-3849	SYM	denotes	-
T26317	3849-3850	HYPH	denotes	/
T26318	3850-3855	NN	denotes	Pygo2
T26319	3855-3856	SYM	denotes	+
T26320	3856-3857	HYPH	denotes	/
T26321	3857-3858	SYM	denotes	-
T26322	3858-3860	,	denotes	,
T26323	3860-3861	DT	denotes	a
T26324	3862-3869	NN	denotes	control
T26325	3870-3879	VBN	denotes	processed
T26326	3880-3882	IN	denotes	in
T26327	3883-3891	NN	denotes	parallel
T26328	3892-3896	IN	denotes	with
T26329	3897-3898	-LRB-	denotes	(
T26330	3898-3899	NN	denotes	K
T26331	3899-3900	-RRB-	denotes	)
T26332	3901-3904	CC	denotes	and
T26333	3905-3906	-LRB-	denotes	(
T26334	3906-3907	NN	denotes	L
T26335	3907-3908	-RRB-	denotes	)
T26336	3908-3910	,	denotes	,
T26337	3910-3914	IN	denotes	with
T26338	3915-3925	NN	denotes	expression
T26339	3926-3928	IN	denotes	in
T26340	3929-3937	JJ	denotes	ureteric
T26341	3938-3942	NN	denotes	tree
T26342	3943-3946	CC	denotes	and
T26343	3947-3962	JJ	denotes	paramesonephric
T26344	3963-3967	NN	denotes	duct
T26345	3967-3968	.	denotes	.
T26346	3968-4199	sentence	denotes	(K, L)Pygo1-/-/Pygo2-/-, reporter activity was lost in the paramesonephric duct (white arrowhead), and in the ureter and ureteric compartment of the developing kidneys (dashed circles), except for (K) a few faintly staining cells.
T26347	3969-3970	-LRB-	denotes	(
T26348	3970-3971	LS	denotes	K
T26350	3971-3973	,	denotes	,
T26351	3973-3974	LS	denotes	L
T26352	3974-3975	-RRB-	denotes	)
T26353	3975-3980	NN	denotes	Pygo1
T26355	3980-3981	SYM	denotes	-
T26356	3981-3982	HYPH	denotes	/
T26357	3982-3983	SYM	denotes	-
T26358	3983-3984	HYPH	denotes	/
T26359	3984-3989	NN	denotes	Pygo2
T26360	3989-3990	SYM	denotes	-
T26361	3990-3991	HYPH	denotes	/
T26362	3991-3992	SYM	denotes	-
T26363	3992-3994	,	denotes	,
T26354	3994-4002	NN	denotes	reporter
T26364	4003-4011	NN	denotes	activity
T26365	4012-4015	VBD	denotes	was
T26349	4016-4020	VBN	denotes	lost
T26366	4021-4023	IN	denotes	in
T26367	4024-4027	DT	denotes	the
T26369	4028-4043	JJ	denotes	paramesonephric
T26368	4044-4048	NN	denotes	duct
T26370	4049-4050	-LRB-	denotes	(
T26372	4050-4055	JJ	denotes	white
T26371	4056-4065	NN	denotes	arrowhead
T26373	4065-4066	-RRB-	denotes	)
T26374	4066-4068	,	denotes	,
T26375	4068-4071	CC	denotes	and
T26376	4072-4074	IN	denotes	in
T26377	4075-4078	DT	denotes	the
T26378	4079-4085	NN	denotes	ureter
T26379	4086-4089	CC	denotes	and
T26380	4090-4098	JJ	denotes	ureteric
T26381	4099-4110	NN	denotes	compartment
T26382	4111-4113	IN	denotes	of
T26383	4114-4117	DT	denotes	the
T26385	4118-4128	VBG	denotes	developing
T26384	4129-4136	NNS	denotes	kidneys
T26386	4137-4138	-LRB-	denotes	(
T26388	4138-4144	VBN	denotes	dashed
T26387	4145-4152	NNS	denotes	circles
T26389	4152-4153	-RRB-	denotes	)
T26390	4153-4155	,	denotes	,
T26391	4155-4161	IN	denotes	except
T26392	4162-4165	IN	denotes	for
T26393	4166-4167	-LRB-	denotes	(
T26394	4167-4168	NN	denotes	K
T26396	4168-4169	-RRB-	denotes	)
T26397	4170-4171	DT	denotes	a
T26398	4172-4175	JJ	denotes	few
T26399	4176-4183	RB	denotes	faintly
T26400	4184-4192	VBG	denotes	staining
T26395	4193-4198	NNS	denotes	cells
T26401	4198-4199	.	denotes	.
T26402	4199-4276	sentence	denotes	(M, N) Pygo1+/-/Pygo2+/- (left) and Pygo1-/-/Pygo2-/- (right) E18.5 kidneys.
T26403	4200-4201	-LRB-	denotes	(
T26404	4201-4202	LS	denotes	M
T26406	4202-4204	,	denotes	,
T26407	4204-4205	LS	denotes	N
T26408	4205-4206	-RRB-	denotes	)
T26409	4207-4212	NN	denotes	Pygo1
T26410	4212-4213	SYM	denotes	+
T26411	4213-4214	HYPH	denotes	/
T26412	4214-4215	SYM	denotes	-
T26413	4215-4216	HYPH	denotes	/
T26414	4216-4221	NN	denotes	Pygo2
T26415	4221-4222	SYM	denotes	+
T26416	4222-4223	HYPH	denotes	/
T26417	4223-4224	SYM	denotes	-
T26418	4225-4226	-LRB-	denotes	(
T26419	4226-4230	JJ	denotes	left
T26420	4230-4231	-RRB-	denotes	)
T26421	4232-4235	CC	denotes	and
T26422	4236-4241	NN	denotes	Pygo1
T26423	4241-4242	SYM	denotes	-
T26424	4242-4243	HYPH	denotes	/
T26425	4243-4244	SYM	denotes	-
T26426	4244-4245	HYPH	denotes	/
T26427	4245-4250	NN	denotes	Pygo2
T26428	4250-4251	SYM	denotes	-
T26429	4251-4252	HYPH	denotes	/
T26430	4252-4253	SYM	denotes	-
T26431	4254-4255	-LRB-	denotes	(
T26432	4255-4260	JJ	denotes	right
T26433	4260-4261	-RRB-	denotes	)
T26434	4262-4267	NN	denotes	E18.5
T26405	4268-4275	NNS	denotes	kidneys
T26435	4275-4276	.	denotes	.
T26436	4276-4310	sentence	denotes	The kidneys in (N) were bisected.
T26437	4277-4280	DT	denotes	The
T26438	4281-4288	NNS	denotes	kidneys
T26440	4289-4291	IN	denotes	in
T26441	4292-4293	-LRB-	denotes	(
T26442	4293-4294	NN	denotes	N
T26443	4294-4295	-RRB-	denotes	)
T26444	4296-4300	VBD	denotes	were
T26439	4301-4309	VBN	denotes	bisected
T26445	4309-4310	.	denotes	.
T26446	4310-4498	sentence	denotes	BAT-gal reporter expression was seen in the ureteric tree components of the cortex and medulla of the double heterozygotes but was almost completely lost in the double-homozygous mutants.
T26447	4311-4314	NN	denotes	BAT
T26449	4314-4315	HYPH	denotes	-
T26448	4315-4318	NN	denotes	gal
T26450	4319-4327	NN	denotes	reporter
T26451	4328-4338	NN	denotes	expression
T26453	4339-4342	VBD	denotes	was
T26452	4343-4347	VBN	denotes	seen
T26454	4348-4350	IN	denotes	in
T26455	4351-4354	DT	denotes	the
T26457	4355-4363	JJ	denotes	ureteric
T26458	4364-4368	NN	denotes	tree
T26456	4369-4379	NNS	denotes	components
T26459	4380-4382	IN	denotes	of
T26460	4383-4386	DT	denotes	the
T26461	4387-4393	NN	denotes	cortex
T26462	4394-4397	CC	denotes	and
T26463	4398-4405	NN	denotes	medulla
T26464	4406-4408	IN	denotes	of
T26465	4409-4412	DT	denotes	the
T26467	4413-4419	JJ	denotes	double
T26466	4420-4433	NNS	denotes	heterozygotes
T26468	4434-4437	CC	denotes	but
T26469	4438-4441	VBD	denotes	was
T26471	4442-4448	RB	denotes	almost
T26472	4449-4459	RB	denotes	completely
T26470	4460-4464	VBN	denotes	lost
T26473	4465-4467	IN	denotes	in
T26474	4468-4471	DT	denotes	the
T26476	4472-4478	JJ	denotes	double
T26478	4478-4479	HYPH	denotes	-
T26477	4479-4489	JJ	denotes	homozygous
T26475	4490-4497	NNS	denotes	mutants
T26479	4497-4498	.	denotes	.
T26480	4498-4601	sentence	denotes	(O) Pygo1+/-/Pygo2+/+ (left), Pygo1-/-/Pygo2+/- (middle), and Pygo1-/-/Pygo2-/- (right) E18.5 kidneys.
T26481	4499-4500	-LRB-	denotes	(
T26482	4500-4501	LS	denotes	O
T26484	4501-4502	-RRB-	denotes	)
T26485	4503-4508	NN	denotes	Pygo1
T26486	4508-4509	SYM	denotes	+
T26487	4509-4510	HYPH	denotes	/
T26488	4510-4511	SYM	denotes	-
T26489	4511-4512	HYPH	denotes	/
T26490	4512-4517	NN	denotes	Pygo2
T26491	4517-4518	SYM	denotes	+
T26492	4518-4519	HYPH	denotes	/
T26493	4519-4520	SYM	denotes	+
T26494	4521-4522	-LRB-	denotes	(
T26495	4522-4526	JJ	denotes	left
T26496	4526-4527	-RRB-	denotes	)
T26497	4527-4529	,	denotes	,
T26498	4529-4534	NN	denotes	Pygo1
T26499	4534-4535	SYM	denotes	-
T26500	4535-4536	HYPH	denotes	/
T26501	4536-4537	SYM	denotes	-
T26502	4537-4538	HYPH	denotes	/
T26503	4538-4543	NN	denotes	Pygo2
T26504	4543-4544	SYM	denotes	+
T26505	4544-4545	HYPH	denotes	/
T26506	4545-4546	SYM	denotes	-
T26507	4547-4548	-LRB-	denotes	(
T26508	4548-4554	JJ	denotes	middle
T26509	4554-4555	-RRB-	denotes	)
T26510	4555-4557	,	denotes	,
T26511	4557-4560	CC	denotes	and
T26512	4561-4566	NN	denotes	Pygo1
T26513	4566-4567	SYM	denotes	-
T26514	4567-4568	HYPH	denotes	/
T26515	4568-4569	SYM	denotes	-
T26516	4569-4570	HYPH	denotes	/
T26517	4570-4575	NN	denotes	Pygo2
T26518	4575-4576	SYM	denotes	-
T26519	4576-4577	HYPH	denotes	/
T26520	4577-4578	SYM	denotes	-
T26521	4579-4580	-LRB-	denotes	(
T26522	4580-4585	JJ	denotes	right
T26523	4585-4586	-RRB-	denotes	)
T26524	4587-4592	NN	denotes	E18.5
T26483	4593-4600	NNS	denotes	kidneys
T26525	4600-4601	.	denotes	.
T26526	4601-4782	sentence	denotes	Reporter activity was present in the ureteric compartments of the Pygo1+/-/Pygo2+/+ (left) and Pygo1-/-/Pygo2+/- (middle) kidneys, but lost in the Pygo1-/-/Pygo2-/- (right) kidney.
T26527	4602-4610	NN	denotes	Reporter
T26528	4611-4619	NN	denotes	activity
T26529	4620-4623	VBD	denotes	was
T26530	4624-4631	JJ	denotes	present
T26531	4632-4634	IN	denotes	in
T26532	4635-4638	DT	denotes	the
T26534	4639-4647	JJ	denotes	ureteric
T26533	4648-4660	NNS	denotes	compartments
T26535	4661-4663	IN	denotes	of
T26536	4664-4667	DT	denotes	the
T26538	4668-4673	NN	denotes	Pygo1
T26539	4673-4674	SYM	denotes	+
T26540	4674-4675	HYPH	denotes	/
T26541	4675-4676	SYM	denotes	-
T26542	4676-4677	HYPH	denotes	/
T26543	4677-4682	NN	denotes	Pygo2
T26544	4682-4683	SYM	denotes	+
T26545	4683-4684	HYPH	denotes	/
T26546	4684-4685	SYM	denotes	+
T26547	4686-4687	-LRB-	denotes	(
T26548	4687-4691	JJ	denotes	left
T26549	4691-4692	-RRB-	denotes	)
T26550	4693-4696	CC	denotes	and
T26551	4697-4702	NN	denotes	Pygo1
T26552	4702-4703	SYM	denotes	-
T26553	4703-4704	HYPH	denotes	/
T26554	4704-4705	SYM	denotes	-
T26555	4705-4706	HYPH	denotes	/
T26556	4706-4711	NN	denotes	Pygo2
T26557	4711-4712	SYM	denotes	+
T26558	4712-4713	HYPH	denotes	/
T26559	4713-4714	SYM	denotes	-
T26560	4715-4716	-LRB-	denotes	(
T26561	4716-4722	JJ	denotes	middle
T26562	4722-4723	-RRB-	denotes	)
T26537	4724-4731	NNS	denotes	kidneys
T26563	4731-4733	,	denotes	,
T26564	4733-4736	CC	denotes	but
T26565	4737-4741	JJ	denotes	lost
T26566	4742-4744	IN	denotes	in
T26567	4745-4748	DT	denotes	the
T26569	4749-4754	NN	denotes	Pygo1
T26570	4754-4755	SYM	denotes	-
T26571	4755-4756	HYPH	denotes	/
T26572	4756-4757	SYM	denotes	-
T26573	4757-4758	HYPH	denotes	/
T26574	4758-4763	NN	denotes	Pygo2
T26575	4763-4764	SYM	denotes	-
T26576	4764-4765	HYPH	denotes	/
T26577	4765-4766	SYM	denotes	-
T26578	4767-4768	-LRB-	denotes	(
T26579	4768-4773	JJ	denotes	right
T26580	4773-4774	-RRB-	denotes	)
T26568	4775-4781	NN	denotes	kidney
T26581	4781-4782	.	denotes	.
T26582	4782-4883	sentence	denotes	Original magnification: (A-C) × 32, (D-F) × 63, (G-I) × 40, (J-L) × 50, (M, N) × 10, and (O) × 12.5.
T26583	4783-4791	JJ	denotes	Original
T26584	4792-4805	NN	denotes	magnification
T26586	4805-4807	:	denotes	:
T26587	4807-4808	-LRB-	denotes	(
T26588	4808-4809	NN	denotes	A
T26589	4809-4810	SYM	denotes	-
T26590	4810-4811	NN	denotes	C
T26591	4811-4812	-RRB-	denotes	)
T26592	4813-4814	SYM	denotes	×
T26585	4815-4817	CD	denotes	32
T26593	4817-4819	,	denotes	,
T26594	4819-4820	-LRB-	denotes	(
T26595	4820-4821	NN	denotes	D
T26597	4821-4822	SYM	denotes	-
T26598	4822-4823	NN	denotes	F
T26599	4823-4824	-RRB-	denotes	)
T26600	4825-4826	SYM	denotes	×
T26596	4827-4829	CD	denotes	63
T26601	4829-4831	,	denotes	,
T26602	4831-4832	-LRB-	denotes	(
T26603	4832-4833	NN	denotes	G
T26605	4833-4834	SYM	denotes	-
T26606	4834-4835	NN	denotes	I
T26607	4835-4836	-RRB-	denotes	)
T26608	4837-4838	SYM	denotes	×
T26604	4839-4841	CD	denotes	40
T26609	4841-4843	,	denotes	,
T26610	4843-4844	-LRB-	denotes	(
T26611	4844-4845	NN	denotes	J
T26613	4845-4846	SYM	denotes	-
T26614	4846-4847	NN	denotes	L
T26615	4847-4848	-RRB-	denotes	)
T26616	4849-4850	SYM	denotes	×
T26612	4851-4853	CD	denotes	50
T26617	4853-4855	,	denotes	,
T26618	4855-4856	-LRB-	denotes	(
T26620	4856-4857	NN	denotes	M
T26621	4857-4859	,	denotes	,
T26619	4859-4860	NN	denotes	N
T26623	4860-4861	-RRB-	denotes	)
T26624	4862-4863	SYM	denotes	×
T26622	4864-4866	CD	denotes	10
T26625	4866-4868	,	denotes	,
T26626	4868-4871	CC	denotes	and
T26627	4872-4873	-LRB-	denotes	(
T26628	4873-4874	NN	denotes	O
T26630	4874-4875	-RRB-	denotes	)
T26631	4876-4877	SYM	denotes	×
T26629	4878-4882	CD	denotes	12.5
T26632	4882-4883	.	denotes	.
T9595	4884-4885	NN	denotes	A
T9594	4884-4887	sentence	denotes	At
T9596	4885-4886	NN	denotes	t
T9598	4887-4889	NN	denotes	E1
T9597	4887-4894	sentence	denotes	E13.5,
T9599	4889-4892	NN	denotes	3.5
T9600	4892-4894	,	denotes	,
T9602	4894-4895	JJ	denotes	t
T9601	4894-4896	sentence	denotes	th
T9603	4895-4896	JJ	denotes	h
T9605	4896-4897	VB	denotes	e
T9604	4896-4905	sentence	denotes	e Pygo2 g
T9606	4898-4903	NN	denotes	Pygo2
T9607	4904-4905	JJ	denotes	g
T9609	4905-4906	NN	denotes	e
T9608	4905-4910	sentence	denotes	ene a
T9610	4906-4907	IN	denotes	n
T9611	4907-4908	DT	denotes	e
T9612	4909-4910	JJ	denotes	a
T9614	4910-4911	NN	denotes	p
T9613	4910-4920	sentence	denotes	ppeared to
T9615	4911-4913	NN	denotes	pe
T9616	4913-4914	CC	denotes	a
T9617	4914-4917	VBN	denotes	red
T9618	4918-4919	DT	denotes	t
T9619	4919-4920	NN	denotes	o
T9620	4920-4921	sentence	denotes
T9622	4921-4922	NN	denotes	p
T9621	4921-4924	sentence	denotes	pla
T9623	4922-4923	VBG	denotes	l
T9624	4923-4924	CC	denotes	a
T9626	4924-4925	NN	denotes	y
T9625	4924-4930	sentence	denotes	y a ma
T9627	4925-4926	,	denotes
T9628	4926-4927	JJ	denotes	a
T9629	4928-4930	NN	denotes	ma
T9632	4930-4933	JJ	denotes	jor
T9630	4930-5056	sentence	denotes	jor role, and the Pygo1 gene a minor role, in canonical Wnt signaling in the ureteric tree, as measured by BAT-gal expression.
T9631	4934-4938	NN	denotes	role
T9633	4938-4940	,	denotes	,
T9634	4940-4943	CC	denotes	and
T9635	4944-4947	DT	denotes	the
T9637	4948-4953	NN	denotes	Pygo1
T9636	4954-4958	NN	denotes	gene
T9639	4959-4960	DT	denotes	a
T9640	4961-4966	JJ	denotes	minor
T9638	4967-4971	NN	denotes	role
T9641	4971-4973	,	denotes	,
T9642	4973-4975	IN	denotes	in
T9643	4976-4985	JJ	denotes	canonical
T9645	4986-4989	NN	denotes	Wnt
T9644	4990-4999	NN	denotes	signaling
T9646	5000-5002	IN	denotes	in
T9647	5003-5006	DT	denotes	the
T9649	5007-5015	JJ	denotes	ureteric
T9648	5016-5020	NN	denotes	tree
T9650	5020-5022	,	denotes	,
T9651	5022-5024	IN	denotes	as
T9652	5025-5033	VBN	denotes	measured
T9653	5034-5036	IN	denotes	by
T9654	5037-5040	NN	denotes	BAT
T9656	5040-5041	HYPH	denotes	-
T9655	5041-5044	NN	denotes	gal
T9657	5045-5055	NN	denotes	expression
T9658	5055-5056	.	denotes	.
T9659	5056-5306	sentence	denotes	A negative control kidney, without the BAT-gal transgene, showed minimal background X-gal staining (Figure 6D), whereas a BAT-gal transgenic kidney with at least one wild-type Pygo2 gene showed strong X-gal staining in the ureteric tree (Figure 6E).
T9660	5057-5058	DT	denotes	A
T9662	5059-5067	JJ	denotes	negative
T9663	5068-5075	NN	denotes	control
T9661	5076-5082	NN	denotes	kidney
T9665	5082-5084	,	denotes	,
T9666	5084-5091	IN	denotes	without
T9667	5092-5095	DT	denotes	the
T9669	5096-5099	NN	denotes	BAT
T9671	5099-5100	HYPH	denotes	-
T9670	5100-5103	NN	denotes	gal
T9668	5104-5113	NN	denotes	transgene
T9672	5113-5115	,	denotes	,
T9664	5115-5121	VBD	denotes	showed
T9673	5122-5129	JJ	denotes	minimal
T9674	5130-5140	NN	denotes	background
T9676	5141-5142	NN	denotes	X
T9678	5142-5143	HYPH	denotes	-
T9677	5143-5146	NN	denotes	gal
T9675	5147-5155	NN	denotes	staining
T9679	5156-5157	-LRB-	denotes	(
T9681	5157-5163	NN	denotes	Figure
T9680	5164-5166	NN	denotes	6D
T9682	5166-5167	-RRB-	denotes	)
T9683	5167-5169	,	denotes	,
T9684	5169-5176	IN	denotes	whereas
T9686	5177-5178	DT	denotes	a
T9688	5179-5182	NN	denotes	BAT
T9690	5182-5183	HYPH	denotes	-
T9689	5183-5186	NN	denotes	gal
T9691	5187-5197	JJ	denotes	transgenic
T9687	5198-5204	NN	denotes	kidney
T9692	5205-5209	IN	denotes	with
T9693	5210-5212	RB	denotes	at
T9695	5213-5218	RBS	denotes	least
T9694	5219-5222	CD	denotes	one
T9697	5223-5227	JJ	denotes	wild
T9699	5227-5228	HYPH	denotes	-
T9698	5228-5232	NN	denotes	type
T9700	5233-5238	NN	denotes	Pygo2
T9696	5239-5243	NN	denotes	gene
T9685	5244-5250	VBD	denotes	showed
T9701	5251-5257	JJ	denotes	strong
T9703	5258-5259	NN	denotes	X
T9705	5259-5260	HYPH	denotes	-
T9704	5260-5263	NN	denotes	gal
T9702	5264-5272	NN	denotes	staining
T9706	5273-5275	IN	denotes	in
T9707	5276-5279	DT	denotes	the
T9709	5280-5288	JJ	denotes	ureteric
T9708	5289-5293	NN	denotes	tree
T9710	5294-5295	-LRB-	denotes	(
T9712	5295-5301	NN	denotes	Figure
T9711	5302-5304	NN	denotes	6E
T9713	5304-5305	-RRB-	denotes	)
T9714	5305-5306	.	denotes	.
T9715	5306-5459	sentence	denotes	In contrast, Pygo1+/-/Pygo2-/- E13.5 kidneys showed very weak reporter expression (Figure 6F), suggesting a significant loss of canonical Wnt signaling.
T9716	5307-5309	IN	denotes	In
T9718	5310-5318	NN	denotes	contrast
T9719	5318-5320	,	denotes	,
T9720	5320-5325	NN	denotes	Pygo1
T9722	5325-5326	SYM	denotes	+
T9723	5326-5327	HYPH	denotes	/
T9724	5327-5328	SYM	denotes	-
T9725	5328-5329	HYPH	denotes	/
T9726	5329-5334	NN	denotes	Pygo2
T9727	5334-5335	SYM	denotes	-
T9728	5335-5336	HYPH	denotes	/
T9729	5336-5337	SYM	denotes	-
T9730	5338-5343	NN	denotes	E13.5
T9721	5344-5351	NNS	denotes	kidneys
T9717	5352-5358	VBD	denotes	showed
T9731	5359-5363	RB	denotes	very
T9732	5364-5368	JJ	denotes	weak
T9734	5369-5377	NN	denotes	reporter
T9733	5378-5388	NN	denotes	expression
T9735	5389-5390	-LRB-	denotes	(
T9737	5390-5396	NN	denotes	Figure
T9736	5397-5399	NN	denotes	6F
T9738	5399-5400	-RRB-	denotes	)
T9739	5400-5402	,	denotes	,
T9740	5402-5412	VBG	denotes	suggesting
T9741	5413-5414	DT	denotes	a
T9743	5415-5426	JJ	denotes	significant
T9742	5427-5431	NN	denotes	loss
T9744	5432-5434	IN	denotes	of
T9745	5435-5444	JJ	denotes	canonical
T9747	5445-5448	NN	denotes	Wnt
T9746	5449-5458	NN	denotes	signaling
T9748	5458-5459	.	denotes	.
T9749	5459-5566	sentence	denotes	Homozygous loss of the Pygo1 gene alone, however, had a small effect on reporter expression (Figure 6G–H).
T9750	5460-5470	JJ	denotes	Homozygous
T9751	5471-5475	NN	denotes	loss
T9753	5476-5478	IN	denotes	of
T9754	5479-5482	DT	denotes	the
T9756	5483-5488	NN	denotes	Pygo1
T9755	5489-5493	NN	denotes	gene
T9757	5494-5499	RB	denotes	alone
T9758	5499-5501	,	denotes	,
T9759	5501-5508	RB	denotes	however
T9760	5508-5510	,	denotes	,
T9752	5510-5513	VBD	denotes	had
T9761	5514-5515	DT	denotes	a
T9763	5516-5521	JJ	denotes	small
T9762	5522-5528	NN	denotes	effect
T9764	5529-5531	IN	denotes	on
T9765	5532-5540	NN	denotes	reporter
T9766	5541-5551	NN	denotes	expression
T9767	5552-5553	-LRB-	denotes	(
T9769	5553-5559	NN	denotes	Figure
T9768	5560-5562	NN	denotes	6G
T9770	5562-5563	SYM	denotes	–
T9771	5563-5564	NN	denotes	H
T9772	5564-5565	-RRB-	denotes	)
T9773	5565-5566	.	denotes	.
T9774	5566-5714	sentence	denotes	Homozygous mutation of both the Pygo1 and Pygo2 genes gave a more dramatic reduction of BAT-gal expression than loss of Pygo2 alone (Figure 6F, L).
T9775	5567-5577	JJ	denotes	Homozygous
T9776	5578-5586	NN	denotes	mutation
T9778	5587-5589	IN	denotes	of
T9779	5590-5594	CC	denotes	both
T9781	5595-5598	DT	denotes	the
T9782	5599-5604	NN	denotes	Pygo1
T9783	5605-5608	CC	denotes	and
T9784	5609-5614	NN	denotes	Pygo2
T9780	5615-5620	NNS	denotes	genes
T9777	5621-5625	VBD	denotes	gave
T9785	5626-5627	DT	denotes	a
T9787	5628-5632	RBR	denotes	more
T9788	5633-5641	JJ	denotes	dramatic
T9786	5642-5651	NN	denotes	reduction
T9789	5652-5654	IN	denotes	of
T9790	5655-5658	NN	denotes	BAT
T9792	5658-5659	HYPH	denotes	-
T9791	5659-5662	NN	denotes	gal
T9793	5663-5673	NN	denotes	expression
T9794	5674-5678	IN	denotes	than
T9795	5679-5683	NN	denotes	loss
T9796	5684-5686	IN	denotes	of
T9797	5687-5692	NN	denotes	Pygo2
T9798	5693-5698	RB	denotes	alone
T9799	5699-5700	-LRB-	denotes	(
T9801	5700-5706	NN	denotes	Figure
T9802	5707-5709	NN	denotes	6F
T9803	5709-5711	,	denotes	,
T9800	5711-5712	NN	denotes	L
T9804	5712-5713	-RRB-	denotes	)
T9805	5713-5714	.	denotes	.
T9806	5714-5833	sentence	denotes	The Pygo1 and Pygo2 genes were also required for BAT-gal reporter expression in the paramesonephric (Mullerian) ducts.
T9807	5715-5718	DT	denotes	The
T9809	5719-5724	NN	denotes	Pygo1
T9810	5725-5728	CC	denotes	and
T9811	5729-5734	NN	denotes	Pygo2
T9808	5735-5740	NNS	denotes	genes
T9813	5741-5745	VBD	denotes	were
T9814	5746-5750	RB	denotes	also
T9812	5751-5759	VBN	denotes	required
T9815	5760-5763	IN	denotes	for
T9816	5764-5767	NN	denotes	BAT
T9818	5767-5768	HYPH	denotes	-
T9817	5768-5771	NN	denotes	gal
T9819	5772-5780	NN	denotes	reporter
T9820	5781-5791	NN	denotes	expression
T9821	5792-5794	IN	denotes	in
T9822	5795-5798	DT	denotes	the
T9824	5799-5814	JJ	denotes	paramesonephric
T9825	5815-5816	-LRB-	denotes	(
T9826	5816-5825	JJ	denotes	Mullerian
T9827	5825-5826	-RRB-	denotes	)
T9823	5827-5832	NNS	denotes	ducts
T9828	5832-5833	.	denotes	.
T9829	5833-6138	sentence	denotes	In Pygo2-/- mice, there was a significant loss of reporter expression (data not shown), and double-homozygous mutants showed loss of X-gal staining in the paramesonephric ducts (Figure 6K), whereas Pygo1-/-/Pygo2+/- and Pygo1+/-/Pygo2+/- mice showed normal levels of BAT-gal expression (Figure 6G, H, J).
T9830	5834-5836	IN	denotes	In
T9832	5837-5842	NN	denotes	Pygo2
T9834	5842-5843	SYM	denotes	-
T9835	5843-5844	HYPH	denotes	/
T9836	5844-5845	SYM	denotes	-
T9833	5846-5850	NNS	denotes	mice
T9837	5850-5852	,	denotes	,
T9838	5852-5857	EX	denotes	there
T9831	5858-5861	VBD	denotes	was
T9839	5862-5863	DT	denotes	a
T9841	5864-5875	JJ	denotes	significant
T9840	5876-5880	NN	denotes	loss
T9842	5881-5883	IN	denotes	of
T9843	5884-5892	NN	denotes	reporter
T9844	5893-5903	NN	denotes	expression
T9845	5904-5905	-LRB-	denotes	(
T9847	5905-5909	NNS	denotes	data
T9848	5910-5913	RB	denotes	not
T9846	5914-5919	VBN	denotes	shown
T9849	5919-5920	-RRB-	denotes	)
T9850	5920-5922	,	denotes	,
T9851	5922-5925	CC	denotes	and
T9852	5926-5932	JJ	denotes	double
T9854	5932-5933	HYPH	denotes	-
T9853	5933-5943	JJ	denotes	homozygous
T9855	5944-5951	NNS	denotes	mutants
T9856	5952-5958	VBD	denotes	showed
T9857	5959-5963	NN	denotes	loss
T9858	5964-5966	IN	denotes	of
T9859	5967-5968	NN	denotes	X
T9861	5968-5969	HYPH	denotes	-
T9860	5969-5972	NN	denotes	gal
T9862	5973-5981	NN	denotes	staining
T9863	5982-5984	IN	denotes	in
T9864	5985-5988	DT	denotes	the
T9866	5989-6004	JJ	denotes	paramesonephric
T9865	6005-6010	NNS	denotes	ducts
T9867	6011-6012	-LRB-	denotes	(
T9869	6012-6018	NN	denotes	Figure
T9868	6019-6021	NN	denotes	6K
T9870	6021-6022	-RRB-	denotes	)
T9871	6022-6024	,	denotes	,
T9872	6024-6031	IN	denotes	whereas
T9874	6032-6037	NN	denotes	Pygo1
T9876	6037-6038	SYM	denotes	-
T9877	6038-6039	HYPH	denotes	/
T9878	6039-6040	SYM	denotes	-
T9879	6040-6041	HYPH	denotes	/
T9880	6041-6046	NN	denotes	Pygo2
T9881	6046-6047	SYM	denotes	+
T9882	6047-6048	HYPH	denotes	/
T9883	6048-6049	SYM	denotes	-
T9884	6050-6053	CC	denotes	and
T9885	6054-6059	NN	denotes	Pygo1
T9886	6059-6060	SYM	denotes	+
T9887	6060-6061	HYPH	denotes	/
T9888	6061-6062	SYM	denotes	-
T9889	6062-6063	HYPH	denotes	/
T9890	6063-6068	NN	denotes	Pygo2
T9891	6068-6069	SYM	denotes	+
T9892	6069-6070	HYPH	denotes	/
T9893	6070-6071	SYM	denotes	-
T9875	6072-6076	NNS	denotes	mice
T9873	6077-6083	VBD	denotes	showed
T9894	6084-6090	JJ	denotes	normal
T9895	6091-6097	NNS	denotes	levels
T9896	6098-6100	IN	denotes	of
T9897	6101-6104	NN	denotes	BAT
T9899	6104-6105	HYPH	denotes	-
T9898	6105-6108	NN	denotes	gal
T9900	6109-6119	NN	denotes	expression
T9901	6120-6121	-LRB-	denotes	(
T9903	6121-6127	NN	denotes	Figure
T9904	6128-6130	NN	denotes	6G
T9905	6130-6132	,	denotes	,
T9906	6132-6133	NN	denotes	H
T9907	6133-6135	,	denotes	,
T9902	6135-6136	NN	denotes	J
T9908	6136-6137	-RRB-	denotes	)
T9909	6137-6138	.	denotes	.
T9910	6138-6380	sentence	denotes	BAT-gal reporter analysis of the Pygo mutants at a later developmental stage, E18.5, also identified a significant decrease in canonical Wnt signaling in the cortical ureteric branches and renal pelvis of the developing kidney (Figure 6M–O).
T9911	6139-6142	NN	denotes	BAT
T9913	6142-6143	HYPH	denotes	-
T9912	6143-6146	NN	denotes	gal
T9914	6147-6155	NN	denotes	reporter
T9915	6156-6164	NN	denotes	analysis
T9917	6165-6167	IN	denotes	of
T9918	6168-6171	DT	denotes	the
T9920	6172-6176	NN	denotes	Pygo
T9919	6177-6184	NNS	denotes	mutants
T9921	6185-6187	IN	denotes	at
T9922	6188-6189	DT	denotes	a
T9924	6190-6195	RB	denotes	later
T9925	6196-6209	JJ	denotes	developmental
T9923	6210-6215	NN	denotes	stage
T9926	6215-6217	,	denotes	,
T9927	6217-6222	NN	denotes	E18.5
T9928	6222-6224	,	denotes	,
T9929	6224-6228	RB	denotes	also
T9916	6229-6239	VBD	denotes	identified
T9930	6240-6241	DT	denotes	a
T9932	6242-6253	JJ	denotes	significant
T9931	6254-6262	NN	denotes	decrease
T9933	6263-6265	IN	denotes	in
T9934	6266-6275	JJ	denotes	canonical
T9936	6276-6279	NN	denotes	Wnt
T9935	6280-6289	NN	denotes	signaling
T9937	6290-6292	IN	denotes	in
T9938	6293-6296	DT	denotes	the
T9940	6297-6305	JJ	denotes	cortical
T9941	6306-6314	JJ	denotes	ureteric
T9939	6315-6323	NNS	denotes	branches
T9942	6324-6327	CC	denotes	and
T9943	6328-6333	JJ	denotes	renal
T9944	6334-6340	NN	denotes	pelvis
T9945	6341-6343	IN	denotes	of
T9946	6344-6347	DT	denotes	the
T9948	6348-6358	VBG	denotes	developing
T9947	6359-6365	NN	denotes	kidney
T9949	6366-6367	-LRB-	denotes	(
T9951	6367-6373	NN	denotes	Figure
T9950	6374-6376	NN	denotes	6M
T9952	6376-6377	SYM	denotes	–
T9953	6377-6378	NN	denotes	O
T9954	6378-6379	-RRB-	denotes	)
T9955	6379-6380	.	denotes	.
T9956	6380-6577	sentence	denotes	Cortical X-gal staining was seen in the ureteric branches of a Pygo1+/-/Pygo2+/- kidney (Figure 6M, left), but was completely absent in the cortex of a Pygo1+/-/Pygo2-/- kidney (Figure 6M, right).
T9957	6381-6389	JJ	denotes	Cortical
T9959	6390-6391	NN	denotes	X
T9961	6391-6392	HYPH	denotes	-
T9960	6392-6395	NN	denotes	gal
T9958	6396-6404	NN	denotes	staining
T9963	6405-6408	VBD	denotes	was
T9962	6409-6413	VBN	denotes	seen
T9964	6414-6416	IN	denotes	in
T9965	6417-6420	DT	denotes	the
T9967	6421-6429	JJ	denotes	ureteric
T9966	6430-6438	NNS	denotes	branches
T9968	6439-6441	IN	denotes	of
T9969	6442-6443	DT	denotes	a
T9971	6444-6449	NN	denotes	Pygo1
T9972	6449-6450	SYM	denotes	+
T9973	6450-6451	HYPH	denotes	/
T9974	6451-6452	SYM	denotes	-
T9975	6452-6453	HYPH	denotes	/
T9976	6453-6458	NN	denotes	Pygo2
T9977	6458-6459	SYM	denotes	+
T9978	6459-6460	HYPH	denotes	/
T9979	6460-6461	SYM	denotes	-
T9970	6462-6468	NN	denotes	kidney
T9980	6469-6470	-LRB-	denotes	(
T9982	6470-6476	NN	denotes	Figure
T9983	6477-6479	NN	denotes	6M
T9984	6479-6481	,	denotes	,
T9981	6481-6485	JJ	denotes	left
T9985	6485-6486	-RRB-	denotes	)
T9986	6486-6488	,	denotes	,
T9987	6488-6491	CC	denotes	but
T9988	6492-6495	VBD	denotes	was
T9989	6496-6506	RB	denotes	completely
T9990	6507-6513	JJ	denotes	absent
T9991	6514-6516	IN	denotes	in
T9992	6517-6520	DT	denotes	the
T9993	6521-6527	NN	denotes	cortex
T9994	6528-6530	IN	denotes	of
T9995	6531-6532	DT	denotes	a
T9997	6533-6538	NN	denotes	Pygo1
T9998	6538-6539	SYM	denotes	+
T9999	6539-6540	HYPH	denotes	/
T10000	6540-6541	SYM	denotes	-
T10001	6541-6542	HYPH	denotes	/
T10002	6542-6547	NN	denotes	Pygo2
T10003	6547-6548	SYM	denotes	-
T10004	6548-6549	HYPH	denotes	/
T10005	6549-6550	SYM	denotes	-
T9996	6551-6557	NN	denotes	kidney
T10006	6558-6559	-LRB-	denotes	(
T10008	6559-6565	NN	denotes	Figure
T10009	6566-6568	NN	denotes	6M
T10010	6568-6570	,	denotes	,
T10007	6570-6575	JJ	denotes	right
T10011	6575-6576	-RRB-	denotes	)
T10012	6576-6577	.	denotes	.
T10013	6577-6752	sentence	denotes	Bisection revealed a significant loss of X-gal staining cells in the collecting ducts and renal pelvis of the Pygo2 null kidney compared with control littermates (Figure 6N).
T10014	6578-6587	NN	denotes	Bisection
T10015	6588-6596	VBD	denotes	revealed
T10016	6597-6598	DT	denotes	a
T10018	6599-6610	JJ	denotes	significant
T10017	6611-6615	NN	denotes	loss
T10019	6616-6618	IN	denotes	of
T10020	6619-6620	NN	denotes	X
T10022	6620-6621	HYPH	denotes	-
T10021	6621-6624	NN	denotes	gal
T10023	6625-6633	VBG	denotes	staining
T10024	6634-6639	NNS	denotes	cells
T10025	6640-6642	IN	denotes	in
T10026	6643-6646	DT	denotes	the
T10028	6647-6657	VBG	denotes	collecting
T10027	6658-6663	NNS	denotes	ducts
T10029	6664-6667	CC	denotes	and
T10030	6668-6673	JJ	denotes	renal
T10031	6674-6680	NN	denotes	pelvis
T10032	6681-6683	IN	denotes	of
T10033	6684-6687	DT	denotes	the
T10035	6688-6693	NN	denotes	Pygo2
T10036	6694-6698	JJ	denotes	null
T10034	6699-6705	NN	denotes	kidney
T10037	6706-6714	VBN	denotes	compared
T10038	6715-6719	IN	denotes	with
T10039	6720-6727	NN	denotes	control
T10040	6728-6739	NNS	denotes	littermates
T10041	6740-6741	-LRB-	denotes	(
T10043	6741-6747	NN	denotes	Figure
T10042	6748-6750	NN	denotes	6N
T10044	6750-6751	-RRB-	denotes	)
T10045	6751-6752	.	denotes	.
T10046	6752-7027	sentence	denotes	Side by side comparison of Pygo1+/+/Pygo2+/- (Figure 6O, left), Pygo1-/-/Pygo2+/- (Figure 6O, middle), and Pygo1-/-/Pygo2-/- (Figure 6O, right) E18.5 kidneys suggested a significant role for the Pygo2 gene in canonical Wnt signaling in the ureteric tree and its derivatives.
T10047	6753-6757	NN	denotes	Side
T10049	6758-6760	IN	denotes	by
T10050	6761-6765	NN	denotes	side
T10048	6766-6776	NN	denotes	comparison
T10052	6777-6779	IN	denotes	of
T10053	6780-6785	NN	denotes	Pygo1
T10055	6785-6786	SYM	denotes	+
T10056	6786-6787	HYPH	denotes	/
T10057	6787-6788	SYM	denotes	+
T10058	6788-6789	HYPH	denotes	/
T10059	6789-6794	NN	denotes	Pygo2
T10060	6794-6795	SYM	denotes	+
T10061	6795-6796	HYPH	denotes	/
T10062	6796-6797	SYM	denotes	-
T10063	6798-6799	-LRB-	denotes	(
T10065	6799-6805	NN	denotes	Figure
T10066	6806-6808	NN	denotes	6O
T10067	6808-6810	,	denotes	,
T10064	6810-6814	JJ	denotes	left
T10068	6814-6815	-RRB-	denotes	)
T10069	6815-6817	,	denotes	,
T10070	6817-6822	NN	denotes	Pygo1
T10071	6822-6823	SYM	denotes	-
T10072	6823-6824	HYPH	denotes	/
T10073	6824-6825	SYM	denotes	-
T10074	6825-6826	HYPH	denotes	/
T10075	6826-6831	NN	denotes	Pygo2
T10076	6831-6832	SYM	denotes	+
T10077	6832-6833	HYPH	denotes	/
T10078	6833-6834	SYM	denotes	-
T10079	6835-6836	-LRB-	denotes	(
T10081	6836-6842	NN	denotes	Figure
T10082	6843-6845	NN	denotes	6O
T10083	6845-6847	,	denotes	,
T10080	6847-6853	JJ	denotes	middle
T10084	6853-6854	-RRB-	denotes	)
T10085	6854-6856	,	denotes	,
T10086	6856-6859	CC	denotes	and
T10087	6860-6865	NN	denotes	Pygo1
T10088	6865-6866	SYM	denotes	-
T10089	6866-6867	HYPH	denotes	/
T10090	6867-6868	SYM	denotes	-
T10091	6868-6869	HYPH	denotes	/
T10092	6869-6874	NN	denotes	Pygo2
T10093	6874-6875	SYM	denotes	-
T10094	6875-6876	HYPH	denotes	/
T10095	6876-6877	SYM	denotes	-
T10096	6878-6879	-LRB-	denotes	(
T10098	6879-6885	NN	denotes	Figure
T10099	6886-6888	NN	denotes	6O
T10100	6888-6890	,	denotes	,
T10097	6890-6895	JJ	denotes	right
T10101	6895-6896	-RRB-	denotes	)
T10102	6897-6902	NN	denotes	E18.5
T10054	6903-6910	NNS	denotes	kidneys
T10051	6911-6920	VBD	denotes	suggested
T10103	6921-6922	DT	denotes	a
T10105	6923-6934	JJ	denotes	significant
T10104	6935-6939	NN	denotes	role
T10106	6940-6943	IN	denotes	for
T10107	6944-6947	DT	denotes	the
T10109	6948-6953	NN	denotes	Pygo2
T10108	6954-6958	NN	denotes	gene
T10110	6959-6961	IN	denotes	in
T10111	6962-6971	JJ	denotes	canonical
T10113	6972-6975	NN	denotes	Wnt
T10112	6976-6985	NN	denotes	signaling
T10114	6986-6988	IN	denotes	in
T10115	6989-6992	DT	denotes	the
T10117	6993-7001	JJ	denotes	ureteric
T10116	7002-7006	NN	denotes	tree
T10118	7007-7010	CC	denotes	and
T10119	7011-7014	PRP$	denotes	its
T10120	7015-7026	NNS	denotes	derivatives
T10121	7026-7027	.	denotes	.
T10122	7027-7248	sentence	denotes	In order to validate and quantify the BAT-gal reporter expression changes in the Pygo2 null and Pygo1/Pygo2 nulls, we performed ELISA measurements of transgene specific β-galactosidase levels in E18.5 kidneys (Figure 7).
T10123	7028-7030	IN	denotes	In
T10125	7031-7036	NN	denotes	order
T10126	7037-7039	TO	denotes	to
T10127	7040-7048	VB	denotes	validate
T10128	7049-7052	CC	denotes	and
T10129	7053-7061	VB	denotes	quantify
T10130	7062-7065	DT	denotes	the
T10132	7066-7069	NN	denotes	BAT
T10134	7069-7070	HYPH	denotes	-
T10133	7070-7073	NN	denotes	gal
T10135	7074-7082	NN	denotes	reporter
T10136	7083-7093	NN	denotes	expression
T10131	7094-7101	NNS	denotes	changes
T10137	7102-7104	IN	denotes	in
T10138	7105-7108	DT	denotes	the
T10140	7109-7114	NN	denotes	Pygo2
T10139	7115-7119	NN	denotes	null
T10141	7120-7123	CC	denotes	and
T10142	7124-7129	NN	denotes	Pygo1
T10144	7129-7130	HYPH	denotes	/
T10143	7130-7135	NN	denotes	Pygo2
T10145	7136-7141	NNS	denotes	nulls
T10146	7141-7143	,	denotes	,
T10147	7143-7145	PRP	denotes	we
T10124	7146-7155	VBD	denotes	performed
T10148	7156-7161	NN	denotes	ELISA
T10149	7162-7174	NNS	denotes	measurements
T10150	7175-7177	IN	denotes	of
T10151	7178-7187	NN	denotes	transgene
T10152	7188-7196	JJ	denotes	specific
T10154	7197-7198	NN	denotes	β
T10156	7198-7199	HYPH	denotes	-
T10155	7199-7212	NN	denotes	galactosidase
T10153	7213-7219	NNS	denotes	levels
T10157	7220-7222	IN	denotes	in
T10158	7223-7228	NN	denotes	E18.5
T10159	7229-7236	NNS	denotes	kidneys
T10160	7237-7238	-LRB-	denotes	(
T10161	7238-7244	NN	denotes	Figure
T10162	7245-7246	CD	denotes	7
T10163	7246-7247	-RRB-	denotes	)
T10164	7247-7248	.	denotes	.
T10165	7248-7368	sentence	denotes	Loss of the Pygo2 gene (Pygo1+/-/Pygo2-/- and Pygo1-/-/Pygo2-/-) gave greater than 90% reduction in BAT-gal expression.
T10166	7249-7253	NN	denotes	Loss
T10168	7254-7256	IN	denotes	of
T10169	7257-7260	DT	denotes	the
T10171	7261-7266	NN	denotes	Pygo2
T10170	7267-7271	NN	denotes	gene
T10172	7272-7273	-LRB-	denotes	(
T10173	7273-7278	NN	denotes	Pygo1
T10174	7278-7279	SYM	denotes	+
T10175	7279-7280	HYPH	denotes	/
T10176	7280-7281	SYM	denotes	-
T10177	7281-7282	HYPH	denotes	/
T10178	7282-7287	NN	denotes	Pygo2
T10179	7287-7288	SYM	denotes	-
T10180	7288-7289	HYPH	denotes	/
T10181	7289-7290	SYM	denotes	-
T10182	7291-7294	CC	denotes	and
T10183	7295-7300	NN	denotes	Pygo1
T10184	7300-7301	SYM	denotes	-
T10185	7301-7302	HYPH	denotes	/
T10186	7302-7303	SYM	denotes	-
T10187	7303-7304	HYPH	denotes	/
T10188	7304-7309	NN	denotes	Pygo2
T10189	7309-7310	SYM	denotes	-
T10190	7310-7311	HYPH	denotes	/
T10191	7311-7312	SYM	denotes	-
T10192	7312-7313	-RRB-	denotes	)
T10167	7314-7318	VBD	denotes	gave
T10193	7319-7326	JJR	denotes	greater
T10195	7327-7331	IN	denotes	than
T10194	7332-7334	CD	denotes	90
T10196	7334-7335	NN	denotes	%
T10197	7336-7345	NN	denotes	reduction
T10198	7346-7348	IN	denotes	in
T10199	7349-7352	NN	denotes	BAT
T10201	7352-7353	HYPH	denotes	-
T10200	7353-7356	NN	denotes	gal
T10202	7357-7367	NN	denotes	expression
T10203	7367-7368	.	denotes	.
T10204	7368-7448	sentence	denotes	Loss of Pygo1 alone (Pygo1-/-/Pygo2+/+) did not result in a significant change.
T10205	7369-7373	NN	denotes	Loss
T10207	7374-7376	IN	denotes	of
T10208	7377-7382	NN	denotes	Pygo1
T10209	7383-7388	RB	denotes	alone
T10210	7389-7390	-LRB-	denotes	(
T10211	7390-7395	NN	denotes	Pygo1
T10212	7395-7396	SYM	denotes	-
T10213	7396-7397	HYPH	denotes	/
T10214	7397-7398	SYM	denotes	-
T10215	7398-7399	HYPH	denotes	/
T10216	7399-7404	NN	denotes	Pygo2
T10217	7404-7405	SYM	denotes	+
T10218	7405-7406	HYPH	denotes	/
T10219	7406-7407	SYM	denotes	+
T10220	7407-7408	-RRB-	denotes	)
T10221	7409-7412	VBD	denotes	did
T10222	7413-7416	RB	denotes	not
T10206	7417-7423	VB	denotes	result
T10223	7424-7426	IN	denotes	in
T10224	7427-7428	DT	denotes	a
T10226	7429-7440	JJ	denotes	significant
T10225	7441-7447	NN	denotes	change
T10227	7447-7448	.	denotes	.
T10228	7448-7612	sentence	denotes	Interestingly, however, the Pygo1-/-/Pygo2+/- showed only 50% of wild-type BAT-gal expression, suggesting a minor contribution by Pygo1 in canonical Wnt signaling.
T10229	7449-7462	RB	denotes	Interestingly
T10231	7462-7464	,	denotes	,
T10232	7464-7471	RB	denotes	however
T10233	7471-7473	,	denotes	,
T10234	7473-7476	DT	denotes	the
T10235	7477-7482	NN	denotes	Pygo1
T10236	7482-7483	SYM	denotes	-
T10237	7483-7484	HYPH	denotes	/
T10238	7484-7485	SYM	denotes	-
T10239	7485-7486	HYPH	denotes	/
T10240	7486-7491	NN	denotes	Pygo2
T10241	7491-7492	SYM	denotes	+
T10242	7492-7493	HYPH	denotes	/
T10243	7493-7494	SYM	denotes	-
T10230	7495-7501	VBD	denotes	showed
T10244	7502-7506	RB	denotes	only
T10245	7507-7509	CD	denotes	50
T10246	7509-7510	NN	denotes	%
T10247	7511-7513	IN	denotes	of
T10248	7514-7518	JJ	denotes	wild
T10250	7518-7519	HYPH	denotes	-
T10249	7519-7523	NN	denotes	type
T10252	7524-7527	NN	denotes	BAT
T10254	7527-7528	HYPH	denotes	-
T10253	7528-7531	NN	denotes	gal
T10251	7532-7542	NN	denotes	expression
T10255	7542-7544	,	denotes	,
T10256	7544-7554	VBG	denotes	suggesting
T10257	7555-7556	DT	denotes	a
T10259	7557-7562	JJ	denotes	minor
T10258	7563-7575	NN	denotes	contribution
T10260	7576-7578	IN	denotes	by
T10261	7579-7584	NN	denotes	Pygo1
T10262	7585-7587	IN	denotes	in
T10263	7588-7597	JJ	denotes	canonical
T10265	7598-7601	NN	denotes	Wnt
T10264	7602-7611	NN	denotes	signaling
T10266	7611-7612	.	denotes	.
T10267	7612-7888	sentence	denotes	Although BAT-gal expression was decreased in the E18.5 Pygo1-/-/Pygo2+/- kidney, confocal analysis of kidneys with this genotype revealed no dilated ureteric tips or significant changes in ureteric tip number per area compared with Pygo1-/-/Pygo2+/+ kidneys (data not shown).
T10268	7613-7621	IN	denotes	Although
T10270	7622-7625	NN	denotes	BAT
T10272	7625-7626	HYPH	denotes	-
T10271	7626-7629	NN	denotes	gal
T10273	7630-7640	NN	denotes	expression
T10274	7641-7644	VBD	denotes	was
T10269	7645-7654	VBN	denotes	decreased
T10276	7655-7657	IN	denotes	in
T10277	7658-7661	DT	denotes	the
T10279	7662-7667	NN	denotes	E18.5
T10280	7668-7673	NN	denotes	Pygo1
T10281	7673-7674	SYM	denotes	-
T10282	7674-7675	HYPH	denotes	/
T10283	7675-7676	SYM	denotes	-
T10284	7676-7677	HYPH	denotes	/
T10285	7677-7682	NN	denotes	Pygo2
T10286	7682-7683	SYM	denotes	+
T10287	7683-7684	HYPH	denotes	/
T10288	7684-7685	SYM	denotes	-
T10278	7686-7692	NN	denotes	kidney
T10289	7692-7694	,	denotes	,
T10290	7694-7702	JJ	denotes	confocal
T10291	7703-7711	NN	denotes	analysis
T10292	7712-7714	IN	denotes	of
T10293	7715-7722	NNS	denotes	kidneys
T10294	7723-7727	IN	denotes	with
T10295	7728-7732	DT	denotes	this
T10296	7733-7741	NN	denotes	genotype
T10275	7742-7750	VBD	denotes	revealed
T10297	7751-7753	DT	denotes	no
T10299	7754-7761	JJ	denotes	dilated
T10300	7762-7770	JJ	denotes	ureteric
T10298	7771-7775	NNS	denotes	tips
T10301	7776-7778	CC	denotes	or
T10302	7779-7790	JJ	denotes	significant
T10303	7791-7798	NNS	denotes	changes
T10304	7799-7801	IN	denotes	in
T10305	7802-7810	JJ	denotes	ureteric
T10306	7811-7814	NN	denotes	tip
T10307	7815-7821	NN	denotes	number
T10308	7822-7825	IN	denotes	per
T10309	7826-7830	NN	denotes	area
T10310	7831-7839	VBN	denotes	compared
T10311	7840-7844	IN	denotes	with
T10312	7845-7850	NN	denotes	Pygo1
T10314	7850-7851	SYM	denotes	-
T10315	7851-7852	HYPH	denotes	/
T10316	7852-7853	SYM	denotes	-
T10317	7853-7854	HYPH	denotes	/
T10318	7854-7859	NN	denotes	Pygo2
T10319	7859-7860	SYM	denotes	+
T10320	7860-7861	HYPH	denotes	/
T10321	7861-7862	SYM	denotes	+
T10313	7863-7870	NNS	denotes	kidneys
T10322	7871-7872	-LRB-	denotes	(
T10324	7872-7876	NNS	denotes	data
T10325	7877-7880	RB	denotes	not
T10323	7881-7886	VBN	denotes	shown
T10326	7886-7887	-RRB-	denotes	)
T10327	7887-7888	.	denotes	.
T10328	7888-8072	sentence	denotes	Collectively, these BAT-gal reporter results suggest a significant role for the Pygo1 and Pygo2 genes in canonical Wnt signaling during development of the ureteric tree of the kidney.
T10329	7889-7901	RB	denotes	Collectively
T10331	7901-7903	,	denotes	,
T10332	7903-7908	DT	denotes	these
T10334	7909-7912	NN	denotes	BAT
T10336	7912-7913	HYPH	denotes	-
T10335	7913-7916	NN	denotes	gal
T10337	7917-7925	NN	denotes	reporter
T10333	7926-7933	NNS	denotes	results
T10330	7934-7941	VBP	denotes	suggest
T10338	7942-7943	DT	denotes	a
T10340	7944-7955	JJ	denotes	significant
T10339	7956-7960	NN	denotes	role
T10341	7961-7964	IN	denotes	for
T10342	7965-7968	DT	denotes	the
T10344	7969-7974	NN	denotes	Pygo1
T10345	7975-7978	CC	denotes	and
T10346	7979-7984	NN	denotes	Pygo2
T10343	7985-7990	NNS	denotes	genes
T10347	7991-7993	IN	denotes	in
T10348	7994-8003	JJ	denotes	canonical
T10350	8004-8007	NN	denotes	Wnt
T10349	8008-8017	NN	denotes	signaling
T10351	8018-8024	IN	denotes	during
T10352	8025-8036	NN	denotes	development
T10353	8037-8039	IN	denotes	of
T10354	8040-8043	DT	denotes	the
T10356	8044-8052	JJ	denotes	ureteric
T10355	8053-8057	NN	denotes	tree
T10357	8058-8060	IN	denotes	of
T10358	8061-8064	DT	denotes	the
T10359	8065-8071	NN	denotes	kidney
T10360	8071-8072	.	denotes	.
T10361	8072-8884	sentence	denotes	Figure 7 Quantitative analysis of BAT-gal reporter expression in Pygo1/Pygo2 E18.5 kidney extracts. Transgene-specific beta-galactosidase was quantified by ELISA analysis. Pygo1+/-/Pygo2+/+, Pygo1+/-/Pygo2+/- and Pygo1+/-/Pygo2+/+ kidneys all showed similar levels of BAT-gal expression. In the Pygo2 hetereozygote, Pygo1 homozygous mutants, however, reporter expression was reduced by about 50%. In Pygo2 homozygous mutants BAT-gal expression was uniformly low, with or without wild-type Pygo1 alleles. Each experimental group included a sample size of at least four. Interestingly, however, even in wild-type mice the BAT-gal reporter showed no expression in the developing metanephric mesenchyme, or metanephric mesenchyme-derived structures, such as renal vesicles, S-shaped bodies, tubules, and glomeruli.
T26917	8083-8095	JJ	denotes	Quantitative
T26918	8096-8104	NN	denotes	analysis
T26919	8105-8107	IN	denotes	of
T26920	8108-8111	NN	denotes	BAT
T26922	8111-8112	HYPH	denotes	-
T26921	8112-8115	NN	denotes	gal
T26923	8116-8124	NN	denotes	reporter
T26924	8125-8135	NN	denotes	expression
T26925	8136-8138	IN	denotes	in
T26926	8139-8144	NN	denotes	Pygo1
T26928	8144-8145	HYPH	denotes	/
T26927	8145-8150	NN	denotes	Pygo2
T26930	8151-8156	NN	denotes	E18.5
T26931	8157-8163	NN	denotes	kidney
T26929	8164-8172	NNS	denotes	extracts
T26932	8172-8173	.	denotes	.
T26933	8173-8245	sentence	denotes	Transgene-specific beta-galactosidase was quantified by ELISA analysis.
T26934	8174-8183	NN	denotes	Transgene
T26936	8183-8184	HYPH	denotes	-
T26935	8184-8192	JJ	denotes	specific
T26938	8193-8197	NN	denotes	beta
T26939	8197-8198	HYPH	denotes	-
T26937	8198-8211	NN	denotes	galactosidase
T26941	8212-8215	VBD	denotes	was
T26940	8216-8226	VBN	denotes	quantified
T26942	8227-8229	IN	denotes	by
T26943	8230-8235	NNP	denotes	ELISA
T26944	8236-8244	NN	denotes	analysis
T26945	8244-8245	.	denotes	.
T26946	8245-8361	sentence	denotes	Pygo1+/-/Pygo2+/+, Pygo1+/-/Pygo2+/- and Pygo1+/-/Pygo2+/+ kidneys all showed similar levels of BAT-gal expression.
T26947	8246-8251	NN	denotes	Pygo1
T26949	8251-8252	SYM	denotes	+
T26950	8252-8253	HYPH	denotes	/
T26951	8253-8254	SYM	denotes	-
T26952	8254-8255	HYPH	denotes	/
T26953	8255-8260	NN	denotes	Pygo2
T26954	8260-8261	SYM	denotes	+
T26955	8261-8262	HYPH	denotes	/
T26956	8262-8263	SYM	denotes	+
T26957	8263-8265	,	denotes	,
T26958	8265-8270	NN	denotes	Pygo1
T26959	8270-8271	SYM	denotes	+
T26960	8271-8272	HYPH	denotes	/
T26961	8272-8273	SYM	denotes	-
T26962	8273-8274	HYPH	denotes	/
T26963	8274-8279	NN	denotes	Pygo2
T26964	8279-8280	SYM	denotes	+
T26965	8280-8281	HYPH	denotes	/
T26966	8281-8282	SYM	denotes	-
T26967	8283-8286	CC	denotes	and
T26968	8287-8292	NN	denotes	Pygo1
T26969	8292-8293	SYM	denotes	+
T26970	8293-8294	HYPH	denotes	/
T26971	8294-8295	SYM	denotes	-
T26972	8295-8296	HYPH	denotes	/
T26973	8296-8301	NN	denotes	Pygo2
T26974	8301-8302	SYM	denotes	+
T26975	8302-8303	HYPH	denotes	/
T26976	8303-8304	SYM	denotes	+
T26948	8305-8312	NNS	denotes	kidneys
T26978	8313-8316	RB	denotes	all
T26977	8317-8323	VBD	denotes	showed
T26979	8324-8331	JJ	denotes	similar
T26980	8332-8338	NNS	denotes	levels
T26981	8339-8341	IN	denotes	of
T26982	8342-8345	NN	denotes	BAT
T26984	8345-8346	HYPH	denotes	-
T26983	8346-8349	NN	denotes	gal
T26985	8350-8360	NN	denotes	expression
T26986	8360-8361	.	denotes	.
T26987	8361-8470	sentence	denotes	In the Pygo2 hetereozygote, Pygo1 homozygous mutants, however, reporter expression was reduced by about 50%.
T26988	8362-8364	IN	denotes	In
T26990	8365-8368	DT	denotes	the
T26992	8369-8374	NN	denotes	Pygo2
T26993	8375-8388	NN	denotes	hetereozygote
T26994	8388-8390	,	denotes	,
T26995	8390-8395	NN	denotes	Pygo1
T26996	8396-8406	JJ	denotes	homozygous
T26991	8407-8414	NNS	denotes	mutants
T26997	8414-8416	,	denotes	,
T26998	8416-8423	RB	denotes	however
T26999	8423-8425	,	denotes	,
T27000	8425-8433	NN	denotes	reporter
T27001	8434-8444	NN	denotes	expression
T27002	8445-8448	VBD	denotes	was
T26989	8449-8456	VBN	denotes	reduced
T27003	8457-8459	IN	denotes	by
T27004	8460-8465	IN	denotes	about
T27005	8466-8468	CD	denotes	50
T27006	8468-8469	NN	denotes	%
T27007	8469-8470	.	denotes	.
T27008	8470-8577	sentence	denotes	In Pygo2 homozygous mutants BAT-gal expression was uniformly low, with or without wild-type Pygo1 alleles.
T27009	8471-8473	IN	denotes	In
T27011	8474-8479	NN	denotes	Pygo2
T27012	8480-8490	JJ	denotes	homozygous
T27013	8491-8498	NNS	denotes	mutants
T27014	8499-8502	NN	denotes	BAT
T27016	8502-8503	HYPH	denotes	-
T27015	8503-8506	NN	denotes	gal
T27017	8507-8517	NN	denotes	expression
T27010	8518-8521	VBD	denotes	was
T27018	8522-8531	RB	denotes	uniformly
T27019	8532-8535	JJ	denotes	low
T27020	8535-8537	,	denotes	,
T27021	8537-8541	IN	denotes	with
T27022	8542-8544	CC	denotes	or
T27023	8545-8552	IN	denotes	without
T27024	8553-8557	JJ	denotes	wild
T27026	8557-8558	HYPH	denotes	-
T27025	8558-8562	NN	denotes	type
T27028	8563-8568	NN	denotes	Pygo1
T27027	8569-8576	NNS	denotes	alleles
T27029	8576-8577	.	denotes	.
T27030	8577-8642	sentence	denotes	Each experimental group included a sample size of at least four.
T27031	8578-8582	DT	denotes	Each
T27033	8583-8595	JJ	denotes	experimental
T27032	8596-8601	NN	denotes	group
T27034	8602-8610	VBD	denotes	included
T27035	8611-8612	DT	denotes	a
T27037	8613-8619	NN	denotes	sample
T27036	8620-8624	NN	denotes	size
T27038	8625-8627	IN	denotes	of
T27039	8628-8630	RB	denotes	at
T27041	8631-8636	RBS	denotes	least
T27040	8637-8641	CD	denotes	four
T27042	8641-8642	.	denotes	.
T10362	8643-8656	RB	denotes	Interestingly
T10364	8656-8658	,	denotes	,
T10365	8658-8665	RB	denotes	however
T10366	8665-8667	,	denotes	,
T10367	8667-8671	RB	denotes	even
T10368	8672-8674	IN	denotes	in
T10369	8675-8679	JJ	denotes	wild
T10371	8679-8680	HYPH	denotes	-
T10370	8680-8684	NN	denotes	type
T10372	8685-8689	NNS	denotes	mice
T10373	8690-8693	DT	denotes	the
T10375	8694-8697	NN	denotes	BAT
T10377	8697-8698	HYPH	denotes	-
T10376	8698-8701	NN	denotes	gal
T10374	8702-8710	NN	denotes	reporter
T10363	8711-8717	VBD	denotes	showed
T10378	8718-8720	DT	denotes	no
T10379	8721-8731	NN	denotes	expression
T10380	8732-8734	IN	denotes	in
T10381	8735-8738	DT	denotes	the
T10383	8739-8749	VBG	denotes	developing
T10384	8750-8761	JJ	denotes	metanephric
T10382	8762-8772	NN	denotes	mesenchyme
T10385	8772-8774	,	denotes	,
T10386	8774-8776	CC	denotes	or
T10387	8777-8788	JJ	denotes	metanephric
T10388	8789-8799	NN	denotes	mesenchyme
T10390	8799-8800	HYPH	denotes	-
T10389	8800-8807	VBN	denotes	derived
T10391	8808-8818	NNS	denotes	structures
T10392	8818-8820	,	denotes	,
T10393	8820-8824	JJ	denotes	such
T10394	8825-8827	IN	denotes	as
T10395	8828-8833	JJ	denotes	renal
T10396	8834-8842	NNS	denotes	vesicles
T10397	8842-8844	,	denotes	,
T10398	8844-8845	NN	denotes	S
T10400	8845-8846	HYPH	denotes	-
T10399	8846-8852	VBN	denotes	shaped
T10401	8853-8859	NNS	denotes	bodies
T10402	8859-8861	,	denotes	,
T10403	8861-8868	NNS	denotes	tubules
T10404	8868-8870	,	denotes	,
T10405	8870-8873	CC	denotes	and
T10406	8874-8883	NNS	denotes	glomeruli
T10407	8883-8884	.	denotes	.
T10408	8884-9026	sentence	denotes	This has been reported previously, and was interpreted to indicate the absence of canonical Wnt signaling in the metanephric mesenchyme [21].
T10409	8885-8889	DT	denotes	This
T10411	8890-8893	VBZ	denotes	has
T10412	8894-8898	VBN	denotes	been
T10410	8899-8907	VBN	denotes	reported
T10413	8908-8918	RB	denotes	previously
T10414	8918-8920	,	denotes	,
T10415	8920-8923	CC	denotes	and
T10416	8924-8927	VBD	denotes	was
T10417	8928-8939	VBN	denotes	interpreted
T10418	8940-8942	TO	denotes	to
T10419	8943-8951	VB	denotes	indicate
T10420	8952-8955	DT	denotes	the
T10421	8956-8963	NN	denotes	absence
T10422	8964-8966	IN	denotes	of
T10423	8967-8976	JJ	denotes	canonical
T10425	8977-8980	NN	denotes	Wnt
T10424	8981-8990	NN	denotes	signaling
T10426	8991-8993	IN	denotes	in
T10427	8994-8997	DT	denotes	the
T10429	8998-9009	JJ	denotes	metanephric
T10428	9010-9020	NN	denotes	mesenchyme
T10430	9021-9022	-LRB-	denotes	[
T10431	9022-9024	CD	denotes	21
T10432	9024-9025	-RRB-	denotes	]
T10433	9025-9026	.	denotes	.
T10434	9026-9144	sentence	denotes	Results from other studies, however, argue for the presence of canonical Wnt signaling in the metanephric mesenchyme.
T10435	9027-9034	NNS	denotes	Results
T10437	9035-9039	IN	denotes	from
T10438	9040-9045	JJ	denotes	other
T10439	9046-9053	NNS	denotes	studies
T10440	9053-9055	,	denotes	,
T10441	9055-9062	RB	denotes	however
T10442	9062-9064	,	denotes	,
T10436	9064-9069	VBP	denotes	argue
T10443	9070-9073	IN	denotes	for
T10444	9074-9077	DT	denotes	the
T10445	9078-9086	NN	denotes	presence
T10446	9087-9089	IN	denotes	of
T10447	9090-9099	JJ	denotes	canonical
T10449	9100-9103	NN	denotes	Wnt
T10448	9104-9113	NN	denotes	signaling
T10450	9114-9116	IN	denotes	in
T10451	9117-9120	DT	denotes	the
T10453	9121-9132	JJ	denotes	metanephric
T10452	9133-9143	NN	denotes	mesenchyme
T10454	9143-9144	.	denotes	.
T10455	9144-9323	sentence	denotes	For example, ureteric bud expression of Wnt1, thought to act through canonical Wnt signaling, can rescue Wnt9b mutants and induce nephrogenesis of the metanephric mesenchyme [2].
T10456	9145-9148	IN	denotes	For
T10458	9149-9156	NN	denotes	example
T10459	9156-9158	,	denotes	,
T10460	9158-9166	JJ	denotes	ureteric
T10461	9167-9170	NN	denotes	bud
T10462	9171-9181	NN	denotes	expression
T10463	9182-9184	IN	denotes	of
T10464	9185-9189	NN	denotes	Wnt1
T10465	9189-9191	,	denotes	,
T10466	9191-9198	VBN	denotes	thought
T10467	9199-9201	TO	denotes	to
T10468	9202-9205	VB	denotes	act
T10469	9206-9213	IN	denotes	through
T10470	9214-9223	JJ	denotes	canonical
T10472	9224-9227	NN	denotes	Wnt
T10471	9228-9237	NN	denotes	signaling
T10473	9237-9239	,	denotes	,
T10474	9239-9242	MD	denotes	can
T10457	9243-9249	VB	denotes	rescue
T10475	9250-9255	NN	denotes	Wnt9b
T10476	9256-9263	NNS	denotes	mutants
T10477	9264-9267	CC	denotes	and
T10478	9268-9274	VB	denotes	induce
T10479	9275-9288	NN	denotes	nephrogenesis
T10480	9289-9291	IN	denotes	of
T10481	9292-9295	DT	denotes	the
T10483	9296-9307	JJ	denotes	metanephric
T10482	9308-9318	NN	denotes	mesenchyme
T10484	9319-9320	-LRB-	denotes	[
T10485	9320-9321	CD	denotes	2
T10486	9321-9322	-RRB-	denotes	]
T10487	9322-9323	.	denotes	.
T10488	9323-9447	sentence	denotes	This suggests that the BAT-gal transgene might not accurately report canonical Wnt signaling in the metanephric mesenchyme.
T10489	9324-9328	DT	denotes	This
T10490	9329-9337	VBZ	denotes	suggests
T10491	9338-9342	IN	denotes	that
T10493	9343-9346	DT	denotes	the
T10495	9347-9350	NN	denotes	BAT
T10497	9350-9351	HYPH	denotes	-
T10496	9351-9354	NN	denotes	gal
T10494	9355-9364	NN	denotes	transgene
T10498	9365-9370	MD	denotes	might
T10499	9371-9374	RB	denotes	not
T10500	9375-9385	RB	denotes	accurately
T10492	9386-9392	VB	denotes	report
T10501	9393-9402	JJ	denotes	canonical
T10503	9403-9406	NN	denotes	Wnt
T10502	9407-9416	NN	denotes	signaling
T10504	9417-9419	IN	denotes	in
T10505	9420-9423	DT	denotes	the
T10507	9424-9435	JJ	denotes	metanephric
T10506	9436-9446	NN	denotes	mesenchyme
T10508	9446-9447	.	denotes	.
T10509	9447-9691	sentence	denotes	To address this question, we incubated E11.5 metanephric mesenchyme in lithium chloride (LiCl), which activates canonical Wnt signaling through inhibition of GSK3, and also functions as an inducer of nephrogenesis in kidney organ culture [22].
T10510	9448-9450	TO	denotes	To
T10511	9451-9458	VB	denotes	address
T10513	9459-9463	DT	denotes	this
T10514	9464-9472	NN	denotes	question
T10515	9472-9474	,	denotes	,
T10516	9474-9476	PRP	denotes	we
T10512	9477-9486	VBD	denotes	incubated
T10517	9487-9492	NN	denotes	E11.5
T10519	9493-9504	JJ	denotes	metanephric
T10518	9505-9515	NN	denotes	mesenchyme
T10520	9516-9518	IN	denotes	in
T10521	9519-9526	NN	denotes	lithium
T10522	9527-9535	NN	denotes	chloride
T10523	9536-9537	-LRB-	denotes	(
T10524	9537-9541	NN	denotes	LiCl
T10525	9541-9542	-RRB-	denotes	)
T10526	9542-9544	,	denotes	,
T10527	9544-9549	WDT	denotes	which
T10528	9550-9559	VBZ	denotes	activates
T10529	9560-9569	JJ	denotes	canonical
T10531	9570-9573	NN	denotes	Wnt
T10530	9574-9583	NN	denotes	signaling
T10532	9584-9591	IN	denotes	through
T10533	9592-9602	NN	denotes	inhibition
T10534	9603-9605	IN	denotes	of
T10535	9606-9610	NN	denotes	GSK3
T10536	9610-9612	,	denotes	,
T10537	9612-9615	CC	denotes	and
T10538	9616-9620	RB	denotes	also
T10539	9621-9630	VBZ	denotes	functions
T10540	9631-9633	IN	denotes	as
T10541	9634-9636	DT	denotes	an
T10542	9637-9644	NN	denotes	inducer
T10543	9645-9647	IN	denotes	of
T10544	9648-9661	NN	denotes	nephrogenesis
T10545	9662-9664	IN	denotes	in
T10546	9665-9671	NN	denotes	kidney
T10547	9672-9677	NN	denotes	organ
T10548	9678-9685	NN	denotes	culture
T10549	9686-9687	-LRB-	denotes	[
T10550	9687-9689	CD	denotes	22
T10551	9689-9690	-RRB-	denotes	]
T10552	9690-9691	.	denotes	.
T10553	9691-9982	sentence	denotes	We observed that LiCl-treated metanephric mesenchyme did undergo nephrogenesis, as expected, but failed to show BAT-gal expression (data not shown), suggesting that this transgene is not an accurate reporter of canonical Wnt signaling in the metanephric mesenchyme of the developing kidney.
T10554	9692-9694	PRP	denotes	We
T10555	9695-9703	VBD	denotes	observed
T10556	9704-9708	IN	denotes	that
T10558	9709-9713	NN	denotes	LiCl
T10560	9713-9714	HYPH	denotes	-
T10559	9714-9721	VBN	denotes	treated
T10562	9722-9733	JJ	denotes	metanephric
T10561	9734-9744	NN	denotes	mesenchyme
T10563	9745-9748	VBD	denotes	did
T10557	9749-9756	VB	denotes	undergo
T10564	9757-9770	NN	denotes	nephrogenesis
T10565	9770-9772	,	denotes	,
T10566	9772-9774	IN	denotes	as
T10567	9775-9783	VBN	denotes	expected
T10568	9783-9785	,	denotes	,
T10569	9785-9788	CC	denotes	but
T10570	9789-9795	VBD	denotes	failed
T10571	9796-9798	TO	denotes	to
T10572	9799-9803	VB	denotes	show
T10573	9804-9807	NN	denotes	BAT
T10575	9807-9808	HYPH	denotes	-
T10574	9808-9811	NN	denotes	gal
T10576	9812-9822	NN	denotes	expression
T10577	9823-9824	-LRB-	denotes	(
T10579	9824-9828	NNS	denotes	data
T10580	9829-9832	RB	denotes	not
T10578	9833-9838	VBN	denotes	shown
T10581	9838-9839	-RRB-	denotes	)
T10582	9839-9841	,	denotes	,
T10583	9841-9851	VBG	denotes	suggesting
T10584	9852-9856	IN	denotes	that
T10586	9857-9861	DT	denotes	this
T10587	9862-9871	NN	denotes	transgene
T10585	9872-9874	VBZ	denotes	is
T10588	9875-9878	RB	denotes	not
T10589	9879-9881	DT	denotes	an
T10591	9882-9890	JJ	denotes	accurate
T10590	9891-9899	NN	denotes	reporter
T10592	9900-9902	IN	denotes	of
T10593	9903-9912	JJ	denotes	canonical
T10595	9913-9916	NN	denotes	Wnt
T10594	9917-9926	NN	denotes	signaling
T10596	9927-9929	IN	denotes	in
T10597	9930-9933	DT	denotes	the
T10599	9934-9945	JJ	denotes	metanephric
T10598	9946-9956	NN	denotes	mesenchyme
T10600	9957-9959	IN	denotes	of
T10601	9960-9963	DT	denotes	the
T10603	9964-9974	VBG	denotes	developing
T10602	9975-9981	NN	denotes	kidney
T10604	9981-9982	.	denotes	.
R1858	T9319	T9320	amod	Reduced,signaling
R1859	T9321	T9320	amod	canonical,signaling
R1860	T9322	T9320	compound	Wnt,signaling
R1861	T9323	T9320	prep	in,signaling
R1862	T9324	T9325	compound	Pygo1,Pygo2
R1863	T9325	T9327	compound	Pygo2,mutant
R1864	T9326	T9325	punct	/,Pygo2
R1865	T9327	T9328	compound	mutant,mice
R1866	T9328	T9323	pobj	mice,in
R1867	T9330	T9331	det	The,reporter
R1868	T9331	T9336	nsubjpass	reporter,used
R1869	T9332	T9333	compound	BAT,gal
R1870	T9333	T9335	compound	gal,transgene
R1871	T9334	T9333	punct	-,gal
R1872	T9335	T9331	compound	transgene,reporter
R1873	T9337	T9331	prep	of,reporter
R1875	T9338	T9339	amod	canonical,signaling
R1877	T9339	T9337	pobj	signaling,of
R1878	T9340	T9339	compound	Wnt,signaling
R1879	T9341	T9342	punct	[,21
R1880	T9342	T9331	parataxis	21,reporter
R1881	T9343	T9342	punct	],21
R1882	T9344	T9336	auxpass	was,used
R1883	T9345	T9346	aux	to,examine
R1884	T9346	T9336	advcl	examine,used
R1885	T9347	T9346	dobj	changes,examine
R1886	T9348	T9347	prep	in,changes
R1887	T9349	T9350	compound	Wnt,signaling
R1888	T9350	T9348	pobj	signaling,in
R1889	T9351	T9347	prep	in,changes
R1890	T9352	T9353	det	the,mice
R1891	T9353	T9351	pobj	mice,in
R1892	T9354	T9353	compound	mutant,mice
R1893	T9410	T9411	mark	while,showed
R1894	T9355	T9336	punct	.,used
R1895	T9411	T9402	advcl	showed,showing
R1896	T9412	T9413	det	the,telencephalon
R1897	T9413	T9411	nsubj	telencephalon,showed
R1898	T9414	T9413	compound	mutant,telencephalon
R1899	T9415	T9411	advmod	still,showed
R1900	T9416	T9417	amod	robust,signaling
R1901	T9417	T9411	dobj	signaling,showed
R1902	T9418	T9417	compound	Wnt,signaling
R1903	T9419	T9385	punct	.,was
R1904	T9421	T9422	det	These,results
R1905	T9422	T9423	nsubj	results,suggest
R1906	T9357	T9358	preconj	Both,Pygo2
R1907	T9424	T9425	mark	that,are
R1908	T9425	T9423	ccomp	are,suggest
R1909	T9426	T9427	det	the,genes
R1910	T9427	T9425	nsubj	genes,are
R1911	T9428	T9427	amod	mammalian,genes
R1912	T9358	T9359	npadvmod	Pygo2,null
R1913	T9429	T9427	compound	Pygo,genes
R1914	T9430	T9431	amod	significant,modulators
R1915	T9431	T9425	attr	modulators,are
R1916	T9432	T9431	prep	of,modulators
R1917	T9359	T9369	amod	null,embryos
R1918	T9433	T9434	amod	canonical,signaling
R1919	T9434	T9432	pobj	signaling,of
R1920	T9360	T9358	punct	-,Pygo2
R1921	T9435	T9434	compound	Wnt,signaling
R1922	T9436	T9425	prep	in,are
R1923	T9437	T9436	pobj	some,in
R1924	T9438	T9436	punct	", ",in
R1925	T9361	T9358	punct	/,Pygo2
R1926	T9439	T9436	cc	but,in
R1927	T9362	T9358	punct	-,Pygo2
R1928	T9440	T9439	neg	not,but
R1929	T9441	T9436	conj	in,in
R1930	T9442	T9441	pobj	all,in
R1931	T9443	T9444	amod	developing,systems
R1932	T9363	T9358	cc	and,Pygo2
R1933	T9444	T9441	pobj	systems,in
R1934	T9445	T9423	punct	.,suggest
R1935	T9364	T9365	compound	Pygo1,Pygo2
R1936	T9365	T9358	conj	Pygo2,Pygo2
R1937	T9366	T9365	punct	/,Pygo2
R1938	T9453	T9452	compound	s,o
R1939	T9367	T9359	amod	double,null
R1940	T9368	T9359	punct	-,null
R1941	T9369	T9371	nsubj	embryos,showed
R1942	T9370	T9369	compound	E10.5,embryos
R1943	T9372	T9373	det	a,decrease
R1944	T9373	T9371	dobj	decrease,showed
R1945	T9374	T9373	prep	in,decrease
R1946	T9375	T9376	amod	canonical,signaling
R1947	T9376	T9374	pobj	signaling,in
R1948	T9465	T9466	compound	BAT,gal
R1949	T9466	T9464	compound	gal,re
R1950	T9467	T9466	punct	-,gal
R1951	T9377	T9376	compound	Wnt,signaling
R1952	T9378	T9379	punct	(,Figure
R1953	T9379	T9371	parataxis	Figure,showed
R1954	T9380	T9379	nummod	5,Figure
R1955	T9381	T9379	punct	),Figure
R1956	T9382	T9371	punct	.,showed
R1957	T9384	T9385	expl	There,was
R1958	T9386	T9385	punct	", ",was
R1959	T9478	T9479	compound	pre,ssion
R1960	T9479	T9477	dobj	ssion,x
R1961	T9387	T9385	advmod	however,was
R1962	T9480	T9477	prep	in,x
R1963	T9481	T9482	amod	more,detail
R1964	T9482	T9480	pobj	detail,in
R1965	T9388	T9385	punct	", ",was
R1966	T9483	T9477	prep	in,x
R1967	T9484	T9485	det	the,system
R1968	T9389	T9390	npadvmod	tissue,specific
R1969	T9390	T9392	amod	specific,variability
R1970	T9485	T9483	pobj	system,in
R1971	T9391	T9390	punct	-,specific
R1972	T9486	T9485	amod	developing,system
R1973	T9487	T9485	amod	urogenital,system
R1974	T9392	T9385	attr	variability,was
R1975	T9488	T9485	prep	of,system
R1976	T9489	T9490	compound	Pygo,mutants
R1977	T9490	T9488	pobj	mutants,of
R1978	T9393	T9392	prep	in,variability
R1979	T9491	T9477	punct	.,x
R1980	T9394	T9395	det	the,degree
R1981	T9493	T9494	det	The,results
R1982	T9395	T9393	pobj	degree,in
R1983	T9396	T9395	prep	of,degree
R1984	T9494	T9495	nsubj	results,suggested
R1985	T9397	T9396	pobj	reduction,of
R1986	T9496	T9497	mark	that,required
R1987	T9497	T9495	ccomp	required,suggested
R1988	T9398	T9385	punct	", ",was
R1989	T9498	T9499	det	the,gene
R1990	T9499	T9497	nsubjpass	gene,required
R1991	T9500	T9499	compound	Pygo2,gene
R1992	T9399	T9385	prep	with,was
R1993	T9501	T9497	auxpass	is,required
R1994	T9502	T9497	prep	for,required
R1995	T9503	T9504	amod	canonical,signaling
R1996	T9400	T9401	det	the,somites
R1997	T9504	T9502	pobj	signaling,for
R1998	T9505	T9504	compound	Wnt,signaling
R1999	T9401	T9402	nsubj	somites,showing
R2000	T9506	T9504	prep	in,signaling
R2001	T9507	T9508	det	the,duct
R2002	T9508	T9506	pobj	duct,in
R2003	T9402	T9399	pcomp	showing,with
R2004	T9403	T9402	punct	", ",showing
R2005	T9509	T9508	amod	nephric,duct
R2006	T9510	T9495	punct	.,suggested
R2007	T9404	T9402	prep	for,showing
R2008	T9512	T9513	preconj	Both,null
R2009	T9513	T9515	amod	null,embryos
R2010	T9405	T9404	pobj	example,for
R2011	T9514	T9513	npadvmod	Pygo2,null
R2012	T9515	T9524	nsubj	embryos,showed
R2013	T9516	T9513	cc	and,null
R2014	T9406	T9402	punct	", ",showing
R2015	T9517	T9518	compound	Pygo1,Pygo2
R2016	T9518	T9520	npadvmod	Pygo2,null
R2017	T9519	T9518	punct	/,Pygo2
R2018	T9407	T9408	amod	strong,reduction
R2019	T9520	T9513	conj	null,null
R2020	T9521	T9520	amod	double,null
R2021	T9522	T9520	punct	-,null
R2022	T9408	T9402	dobj	reduction,showing
R2023	T9523	T9515	compound	E10.5,embryos
R2024	T9525	T9526	det	an,absence
R2025	T9409	T9402	punct	", ",showing
R2026	T9526	T9524	dobj	absence,showed
R2027	T9527	T9526	prep	of,absence
R2028	T9528	T9529	compound	reporter,expression
R2029	T9591	T9587	punct	),6A
R2030	T9529	T9527	pobj	expression,of
R2031	T9530	T9526	prep	in,absence
R2032	T9531	T9532	det	the,duct
R2033	T9592	T9550	punct	.,form
R2034	T9532	T9530	pobj	duct,in
R2035	T9533	T9532	amod	nephric,duct
R2036	T9534	T9524	punct	", ",showed
R2037	T9599	T9598	conj	3.5,E1
R2038	T9535	T9536	mark	while,showed
R2039	T9536	T9524	advcl	showed,showed
R2040	T9537	T9538	compound	control,littermates
R2041	T9538	T9536	nsubj	littermates,showed
R2042	T9539	T9540	amod	strong,expression
R2043	T9540	T9536	dobj	expression,showed
R2044	T9541	T9542	punct	(,6A
R2045	T9542	T9524	parataxis	6A,showed
R2046	T9543	T9542	compound	Figure,6A
R2047	T9544	T9545	punct	–,C
R2048	T9545	T9542	prep	C,6A
R2049	T9546	T9542	punct	),6A
R2050	T9614	T9615	compound	p,pe
R2051	T9547	T9524	punct	.,showed
R2052	T9549	T9550	prep	In,form
R2053	T9551	T9552	compound	Pygo1,Pygo2
R2054	T9552	T9554	npadvmod	Pygo2,null
R2055	T9553	T9552	punct	/,Pygo2
R2056	T9554	T9557	amod	null,mutants
R2057	T9555	T9554	amod	double,null
R2058	T9556	T9554	punct	-,null
R2059	T9557	T9549	pobj	mutants,In
R2060	T9558	T9550	punct	", ",form
R2061	T9559	T9560	det	the,duct
R2062	T9560	T9550	nsubj	duct,form
R2063	T9561	T9560	amod	nephric,duct
R2064	T9562	T9550	aux	did,form
R2065	T9563	T9550	punct	", ",form
R2066	T9564	T9550	advmod	however,form
R2067	T9565	T9550	punct	", ",form
R2068	T9566	T9550	cc	and,form
R2069	T9567	T9550	conj	give,form
R2070	T9568	T9567	dobj	rise,give
R2071	T9569	T9567	prep	to,give
R2072	T9570	T9571	det	the,outgrowth
R2073	T9571	T9569	pobj	outgrowth,to
R2074	T9572	T9573	amod	ureteric,bud
R2075	T9573	T9571	compound	bud,outgrowth
R2076	T9574	T9571	punct	", ",outgrowth
R2077	T9627	T9626	punct	,y
R2078	T9575	T9576	dep	which,showed
R2079	T9576	T9571	relcl	showed,outgrowth
R2080	T9577	T9578	amod	reduced,expression
R2081	T9578	T9576	dobj	expression,showed
R2082	T9579	T9577	cc	but,reduced
R2083	T9580	T9579	neg	not,but
R2084	T9581	T9577	conj	absent,reduced
R2085	T9582	T9583	compound	BAT,gal
R2086	T9583	T9585	compound	gal,reporter
R2087	T9584	T9583	punct	-,gal
R2088	T9585	T9578	compound	reporter,expression
R2089	T9586	T9587	punct	(,6A
R2090	T9587	T9576	parataxis	6A,showed
R2091	T9588	T9587	compound	Figure,6A
R2092	T9589	T9590	punct	–,C
R2093	T9632	T9631	amod	jor,role
R2094	T9590	T9587	prep	C,6A
R2095	T9635	T9636	det	the,gene
R2096	T9636	T9638	nsubj	gene,role
R2097	T9637	T9636	compound	Pygo1,gene
R2098	T9639	T9638	det	a,role
R2099	T9643	T9644	amod	canonical,signaling
R2100	T9640	T9638	amod	minor,role
R2101	T9644	T9642	pobj	signaling,in
R2102	T9645	T9644	compound	Wnt,signaling
R2103	T9641	T9638	punct	", ",role
R2104	T9646	T9644	prep	in,signaling
R2105	T9647	T9648	det	the,tree
R2106	T9648	T9646	pobj	tree,in
R2107	T9649	T9648	amod	ureteric,tree
R2108	T9651	T9652	mark	as,measured
R2109	T9642	T9638	conj	in,role
R2110	T9653	T9652	agent	by,measured
R2111	T9654	T9655	compound	BAT,gal
R2112	T9655	T9657	compound	gal,expression
R2113	T9656	T9655	punct	-,gal
R2114	T9748	T9717	punct	.,showed
R2115	T9657	T9653	pobj	expression,by
R2116	T9750	T9751	amod	Homozygous,loss
R2117	T9660	T9661	det	A,kidney
R2118	T9661	T9664	nsubj	kidney,showed
R2119	T9662	T9661	amod	negative,kidney
R2120	T9663	T9661	compound	control,kidney
R2121	T9751	T9752	nsubj	loss,had
R2122	T9665	T9661	punct	", ",kidney
R2123	T9666	T9661	prep	without,kidney
R2124	T9753	T9751	prep	of,loss
R2125	T9667	T9668	det	the,transgene
R2126	T9668	T9666	pobj	transgene,without
R2127	T9669	T9670	compound	BAT,gal
R2128	T9670	T9668	compound	gal,transgene
R2129	T9754	T9755	det	the,gene
R2130	T9671	T9670	punct	-,gal
R2131	T9672	T9664	punct	", ",showed
R2132	T9673	T9674	amod	minimal,background
R2133	T9755	T9753	pobj	gene,of
R2134	T9674	T9675	compound	background,staining
R2135	T9756	T9755	compound	Pygo1,gene
R2136	T9675	T9664	dobj	staining,showed
R2137	T9676	T9677	compound	X,gal
R2138	T9757	T9755	advmod	alone,gene
R2139	T9677	T9675	compound	gal,staining
R2140	T9678	T9677	punct	-,gal
R2141	T9679	T9680	punct	(,6D
R2142	T9758	T9752	punct	", ",had
R2143	T9680	T9675	parataxis	6D,staining
R2144	T9681	T9680	compound	Figure,6D
R2145	T9759	T9752	advmod	however,had
R2146	T9682	T9680	punct	),6D
R2147	T9683	T9664	punct	", ",showed
R2148	T9760	T9752	punct	", ",had
R2149	T9684	T9685	mark	whereas,showed
R2150	T9685	T9664	advcl	showed,showed
R2151	T9761	T9762	det	a,effect
R2152	T9686	T9687	det	a,kidney
R2153	T9687	T9685	nsubj	kidney,showed
R2154	T9688	T9689	compound	BAT,gal
R2155	T9689	T9691	npadvmod	gal,transgenic
R2156	T9762	T9752	dobj	effect,had
R2157	T9690	T9689	punct	-,gal
R2158	T9691	T9687	amod	transgenic,kidney
R2159	T9692	T9687	prep	with,kidney
R2160	T9693	T9694	advmod	at,one
R2161	T9694	T9696	nummod	one,gene
R2162	T9695	T9694	advmod	least,one
R2163	T9763	T9762	amod	small,effect
R2164	T9696	T9692	pobj	gene,with
R2165	T9697	T9698	amod	wild,type
R2166	T9698	T9696	compound	type,gene
R2167	T9764	T9762	prep	on,effect
R2168	T9699	T9698	punct	-,type
R2169	T9700	T9696	compound	Pygo2,gene
R2170	T9765	T9766	compound	reporter,expression
R2171	T9701	T9702	amod	strong,staining
R2172	T9702	T9685	dobj	staining,showed
R2173	T9703	T9704	compound	X,gal
R2174	T9766	T9764	pobj	expression,on
R2175	T9704	T9702	compound	gal,staining
R2176	T9705	T9704	punct	-,gal
R2177	T9706	T9702	prep	in,staining
R2178	T9767	T9768	punct	(,6G
R2179	T9707	T9708	det	the,tree
R2180	T9708	T9706	pobj	tree,in
R2181	T9709	T9708	amod	ureteric,tree
R2182	T9768	T9752	parataxis	6G,had
R2183	T9710	T9711	punct	(,6E
R2184	T9711	T9702	parataxis	6E,staining
R2185	T9712	T9711	compound	Figure,6E
R2186	T9713	T9711	punct	),6E
R2187	T9769	T9768	compound	Figure,6G
R2188	T9714	T9664	punct	.,showed
R2189	T9770	T9771	punct	–,H
R2190	T9716	T9717	prep	In,showed
R2191	T9718	T9716	pobj	contrast,In
R2192	T9771	T9768	prep	H,6G
R2193	T9719	T9717	punct	", ",showed
R2194	T9720	T9721	nmod	Pygo1,kidneys
R2195	T9721	T9717	nsubj	kidneys,showed
R2196	T9772	T9768	punct	),6G
R2197	T9722	T9720	punct	+,Pygo1
R2198	T9723	T9720	punct	/,Pygo1
R2199	T9724	T9720	punct	-,Pygo1
R2200	T9773	T9752	punct	.,had
R2201	T9725	T9720	punct	/,Pygo1
R2202	T9726	T9720	appos	Pygo2,Pygo1
R2203	T9727	T9726	punct	-,Pygo2
R2204	T9775	T9776	amod	Homozygous,mutation
R2205	T9728	T9726	punct	/,Pygo2
R2206	T9729	T9726	punct	-,Pygo2
R2207	T9730	T9721	compound	E13.5,kidneys
R2208	T9776	T9777	nsubj	mutation,gave
R2209	T9731	T9732	advmod	very,weak
R2210	T9732	T9733	amod	weak,expression
R2211	T9733	T9717	dobj	expression,showed
R2212	T9734	T9733	compound	reporter,expression
R2213	T9735	T9736	punct	(,6F
R2214	T9736	T9733	parataxis	6F,expression
R2215	T9778	T9776	prep	of,mutation
R2216	T9737	T9736	compound	Figure,6F
R2217	T9738	T9736	punct	),6F
R2218	T9739	T9717	punct	", ",showed
R2219	T9740	T9717	advcl	suggesting,showed
R2220	T9741	T9742	det	a,loss
R2221	T9779	T9780	preconj	both,genes
R2222	T9742	T9740	dobj	loss,suggesting
R2223	T9743	T9742	amod	significant,loss
R2224	T9744	T9742	prep	of,loss
R2225	T9780	T9778	pobj	genes,of
R2226	T9745	T9746	amod	canonical,signaling
R2227	T9746	T9744	pobj	signaling,of
R2228	T9781	T9780	det	the,genes
R2229	T9747	T9746	compound	Wnt,signaling
R2230	T9782	T9780	nmod	Pygo1,genes
R2231	T9783	T9782	cc	and,Pygo1
R2232	T9784	T9782	conj	Pygo2,Pygo1
R2233	T9853	T9855	amod	homozygous,mutants
R2234	T9785	T9786	det	a,reduction
R2235	T9854	T9853	punct	-,homozygous
R2236	T9855	T9856	nsubj	mutants,showed
R2237	T9786	T9777	dobj	reduction,gave
R2238	T9856	T9831	conj	showed,was
R2239	T9857	T9856	dobj	loss,showed
R2240	T9787	T9788	advmod	more,dramatic
R2241	T9858	T9857	prep	of,loss
R2242	T9859	T9860	compound	X,gal
R2243	T9788	T9786	amod	dramatic,reduction
R2244	T9860	T9862	compound	gal,staining
R2245	T9861	T9860	punct	-,gal
R2246	T9862	T9858	pobj	staining,of
R2247	T9789	T9786	prep	of,reduction
R2248	T9863	T9856	prep	in,showed
R2249	T9864	T9865	det	the,ducts
R2250	T9865	T9863	pobj	ducts,in
R2251	T9790	T9791	compound	BAT,gal
R2252	T9866	T9865	amod	paramesonephric,ducts
R2253	T9867	T9868	punct	(,6K
R2254	T9868	T9865	parataxis	6K,ducts
R2255	T9791	T9793	compound	gal,expression
R2256	T9869	T9868	compound	Figure,6K
R2257	T9870	T9868	punct	),6K
R2258	T9871	T9856	punct	", ",showed
R2259	T9792	T9791	punct	-,gal
R2260	T9872	T9873	mark	whereas,showed
R2261	T9873	T9856	advcl	showed,showed
R2262	T9793	T9789	pobj	expression,of
R2263	T9874	T9875	nmod	Pygo1,mice
R2264	T9875	T9873	nsubj	mice,showed
R2265	T9876	T9874	punct	-,Pygo1
R2266	T9877	T9874	punct	/,Pygo1
R2267	T9878	T9874	punct	-,Pygo1
R2268	T9879	T9874	punct	/,Pygo1
R2269	T9794	T9786	prep	than,reduction
R2270	T9880	T9874	appos	Pygo2,Pygo1
R2271	T9881	T9880	punct	+,Pygo2
R2272	T9882	T9880	punct	/,Pygo2
R2273	T9883	T9880	punct	-,Pygo2
R2274	T9795	T9794	pobj	loss,than
R2275	T9884	T9874	cc	and,Pygo1
R2276	T9885	T9874	conj	Pygo1,Pygo1
R2277	T9796	T9795	prep	of,loss
R2278	T9886	T9885	punct	+,Pygo1
R2279	T9887	T9885	punct	/,Pygo1
R2280	T9888	T9885	punct	-,Pygo1
R2281	T9797	T9796	pobj	Pygo2,of
R2282	T9889	T9885	punct	/,Pygo1
R2283	T9890	T9885	appos	Pygo2,Pygo1
R2284	T9891	T9890	punct	+,Pygo2
R2285	T9892	T9890	punct	/,Pygo2
R2286	T9798	T9795	advmod	alone,loss
R2287	T9893	T9890	punct	-,Pygo2
R2288	T9894	T9895	amod	normal,levels
R2289	T9895	T9873	dobj	levels,showed
R2290	T9799	T9800	punct	(,L
R2291	T9896	T9895	prep	of,levels
R2292	T9897	T9898	compound	BAT,gal
R2293	T9898	T9900	compound	gal,expression
R2294	T9800	T9786	parataxis	L,reduction
R2295	T9899	T9898	punct	-,gal
R2296	T9900	T9896	pobj	expression,of
R2297	T9901	T9902	punct	(,J
R2298	T9801	T9800	nmod	Figure,L
R2299	T9902	T9873	parataxis	J,showed
R2300	T9903	T9902	nmod	Figure,J
R2301	T9802	T9800	nmod	6F,L
R2302	T9904	T9902	nmod	6G,J
R2303	T9905	T9902	punct	", ",J
R2304	T9906	T9902	nmod	H,J
R2305	T9803	T9800	punct	", ",L
R2306	T9907	T9902	punct	", ",J
R2307	T9908	T9902	punct	),J
R2308	T9909	T9856	punct	.,showed
R2309	T9804	T9800	punct	),L
R2310	T9911	T9912	compound	BAT,gal
R2311	T9912	T9914	compound	gal,reporter
R2312	T9913	T9912	punct	-,gal
R2313	T9805	T9777	punct	.,gave
R2314	T9914	T9915	compound	reporter,analysis
R2315	T9915	T9916	nsubj	analysis,identified
R2316	T9917	T9915	prep	of,analysis
R2317	T9918	T9919	det	the,mutants
R2318	T9807	T9808	det	The,genes
R2319	T9919	T9917	pobj	mutants,of
R2320	T9920	T9919	compound	Pygo,mutants
R2321	T9921	T9915	prep	at,analysis
R2322	T9922	T9923	det	a,stage
R2323	T9923	T9921	pobj	stage,at
R2324	T9924	T9923	advmod	later,stage
R2325	T9808	T9812	nsubjpass	genes,required
R2326	T9925	T9923	amod	developmental,stage
R2327	T9926	T9923	punct	", ",stage
R2328	T9927	T9923	appos	E18.5,stage
R2329	T9809	T9808	nmod	Pygo1,genes
R2330	T9928	T9916	punct	", ",identified
R2331	T9929	T9916	advmod	also,identified
R2332	T9930	T9931	det	a,decrease
R2333	T9810	T9809	cc	and,Pygo1
R2334	T9811	T9809	conj	Pygo2,Pygo1
R2335	T9931	T9916	dobj	decrease,identified
R2336	T9932	T9931	amod	significant,decrease
R2337	T9813	T9812	auxpass	were,required
R2338	T9933	T9931	prep	in,decrease
R2339	T9934	T9935	amod	canonical,signaling
R2340	T9935	T9933	pobj	signaling,in
R2341	T9936	T9935	compound	Wnt,signaling
R2342	T9937	T9931	prep	in,decrease
R2343	T9814	T9812	advmod	also,required
R2344	T9938	T9939	det	the,branches
R2345	T9939	T9937	pobj	branches,in
R2346	T9815	T9812	prep	for,required
R2347	T9940	T9939	amod	cortical,branches
R2348	T9941	T9939	amod	ureteric,branches
R2349	T9942	T9939	cc	and,branches
R2350	T9816	T9817	compound	BAT,gal
R2351	T9943	T9944	amod	renal,pelvis
R2352	T9944	T9939	conj	pelvis,branches
R2353	T9945	T9939	prep	of,branches
R2354	T9817	T9819	compound	gal,reporter
R2355	T9946	T9947	det	the,kidney
R2356	T9947	T9945	pobj	kidney,of
R2357	T9948	T9947	amod	developing,kidney
R2358	T9818	T9817	punct	-,gal
R2359	T9949	T9950	punct	(,6M
R2360	T9950	T9916	parataxis	6M,identified
R2361	T9951	T9950	compound	Figure,6M
R2362	T9819	T9820	compound	reporter,expression
R2363	T9952	T9953	punct	–,O
R2364	T9953	T9950	prep	O,6M
R2365	T9820	T9815	pobj	expression,for
R2366	T9954	T9950	punct	),6M
R2367	T9955	T9916	punct	.,identified
R2368	T9957	T9958	amod	Cortical,staining
R2369	T9821	T9820	prep	in,expression
R2370	T9822	T9823	det	the,ducts
R2371	T9958	T9962	nsubjpass	staining,seen
R2372	T9823	T9821	pobj	ducts,in
R2373	T9959	T9960	compound	X,gal
R2374	T9960	T9958	compound	gal,staining
R2375	T9961	T9960	punct	-,gal
R2376	T9824	T9823	amod	paramesonephric,ducts
R2377	T9963	T9962	auxpass	was,seen
R2378	T9964	T9962	prep	in,seen
R2379	T9825	T9826	punct	(,Mullerian
R2380	T9965	T9966	det	the,branches
R2381	T9966	T9964	pobj	branches,in
R2382	T9967	T9966	amod	ureteric,branches
R2383	T9826	T9824	parataxis	Mullerian,paramesonephric
R2384	T9968	T9966	prep	of,branches
R2385	T9969	T9970	det	a,kidney
R2386	T9970	T9968	pobj	kidney,of
R2387	T9827	T9826	punct	),Mullerian
R2388	T9971	T9970	nmod	Pygo1,kidney
R2389	T9972	T9971	punct	+,Pygo1
R2390	T9973	T9971	punct	/,Pygo1
R2391	T9828	T9812	punct	.,required
R2392	T9974	T9971	punct	-,Pygo1
R2393	T9975	T9971	punct	/,Pygo1
R2394	T9976	T9971	appos	Pygo2,Pygo1
R2395	T9830	T9831	prep	In,was
R2396	T9977	T9976	punct	+,Pygo2
R2397	T9978	T9976	punct	/,Pygo2
R2398	T9979	T9976	punct	-,Pygo2
R2399	T9980	T9981	punct	(,left
R2400	T9981	T9962	parataxis	left,seen
R2401	T9832	T9833	nmod	Pygo2,mice
R2402	T9982	T9983	compound	Figure,6M
R2403	T9983	T9981	dep	6M,left
R2404	T9984	T9981	punct	", ",left
R2405	T9985	T9981	punct	),left
R2406	T9833	T9830	pobj	mice,In
R2407	T9986	T9962	punct	", ",seen
R2408	T9987	T9962	cc	but,seen
R2409	T9988	T9962	conj	was,seen
R2410	T9834	T9832	punct	-,Pygo2
R2411	T9989	T9990	advmod	completely,absent
R2412	T9990	T9988	acomp	absent,was
R2413	T9991	T9988	prep	in,was
R2414	T9835	T9832	punct	/,Pygo2
R2415	T9992	T9993	det	the,cortex
R2416	T9836	T9832	punct	-,Pygo2
R2417	T9993	T9991	pobj	cortex,in
R2418	T9837	T9831	punct	", ",was
R2419	T9994	T9993	prep	of,cortex
R2420	T9995	T9996	det	a,kidney
R2421	T9996	T9994	pobj	kidney,of
R2422	T9997	T9996	nmod	Pygo1,kidney
R2423	T9838	T9831	expl	there,was
R2424	T9998	T9997	punct	+,Pygo1
R2425	T9999	T9997	punct	/,Pygo1
R2426	T10000	T9997	punct	-,Pygo1
R2427	T9839	T9840	det	a,loss
R2428	T10001	T9997	punct	/,Pygo1
R2429	T10002	T9997	appos	Pygo2,Pygo1
R2430	T10003	T10002	punct	-,Pygo2
R2431	T9840	T9831	attr	loss,was
R2432	T10004	T10002	punct	/,Pygo2
R2433	T10005	T10002	punct	-,Pygo2
R2434	T10006	T10007	punct	(,right
R2435	T9841	T9840	amod	significant,loss
R2436	T10007	T9988	parataxis	right,was
R2437	T10008	T10009	compound	Figure,6M
R2438	T10009	T10007	dep	6M,right
R2439	T9842	T9840	prep	of,loss
R2440	T10010	T10007	punct	", ",right
R2441	T10011	T10007	punct	),right
R2442	T10012	T9962	punct	.,seen
R2443	T9843	T9844	compound	reporter,expression
R2444	T10014	T10015	nsubj	Bisection,revealed
R2445	T9844	T9842	pobj	expression,of
R2446	T10016	T10017	det	a,loss
R2447	T9845	T9846	punct	(,shown
R2448	T9846	T9831	parataxis	shown,was
R2449	T10017	T10015	dobj	loss,revealed
R2450	T10018	T10017	amod	significant,loss
R2451	T10019	T10017	prep	of,loss
R2452	T9847	T9846	nsubj	data,shown
R2453	T10020	T10021	compound	X,gal
R2454	T10021	T10023	npadvmod	gal,staining
R2455	T10022	T10021	punct	-,gal
R2456	T9848	T9846	neg	not,shown
R2457	T10023	T10024	amod	staining,cells
R2458	T10024	T10019	pobj	cells,of
R2459	T9849	T9846	punct	),shown
R2460	T10025	T10017	prep	in,loss
R2461	T10026	T10027	det	the,ducts
R2462	T10027	T10025	pobj	ducts,in
R2463	T9850	T9831	punct	", ",was
R2464	T10028	T10027	amod	collecting,ducts
R2465	T10029	T10027	cc	and,ducts
R2466	T10030	T10031	amod	renal,pelvis
R2467	T10031	T10027	conj	pelvis,ducts
R2468	T9851	T9831	cc	and,was
R2469	T10032	T10027	prep	of,ducts
R2470	T10033	T10034	det	the,kidney
R2471	T10034	T10032	pobj	kidney,of
R2472	T10035	T10036	npadvmod	Pygo2,null
R2473	T10036	T10034	amod	null,kidney
R2474	T9852	T9853	amod	double,homozygous
R2475	T10037	T10017	prep	compared,loss
R2476	T10038	T10037	prep	with,compared
R2477	T10039	T10040	compound	control,littermates
R2478	T10040	T10038	pobj	littermates,with
R2479	T10041	T10042	punct	(,6N
R2480	T10063	T10064	punct	(,left
R2481	T10042	T10015	parataxis	6N,revealed
R2482	T10043	T10042	compound	Figure,6N
R2483	T10044	T10042	punct	),6N
R2484	T10064	T10053	parataxis	left,Pygo1
R2485	T10045	T10015	punct	.,revealed
R2486	T10047	T10048	nmod	Side,comparison
R2487	T10048	T10051	nsubj	comparison,suggested
R2488	T10065	T10066	compound	Figure,6O
R2489	T10049	T10047	prep	by,Side
R2490	T10050	T10049	pobj	side,by
R2491	T10066	T10064	dep	6O,left
R2492	T10052	T10048	prep	of,comparison
R2493	T10053	T10054	nmod	Pygo1,kidneys
R2494	T10067	T10064	punct	", ",left
R2495	T10068	T10064	punct	),left
R2496	T10054	T10052	pobj	kidneys,of
R2497	T10055	T10053	punct	+,Pygo1
R2498	T10069	T10053	punct	", ",Pygo1
R2499	T10056	T10053	punct	/,Pygo1
R2500	T10057	T10053	punct	+,Pygo1
R2501	T10058	T10053	punct	/,Pygo1
R2502	T10070	T10053	conj	Pygo1,Pygo1
R2503	T10059	T10053	appos	Pygo2,Pygo1
R2504	T10060	T10059	punct	+,Pygo2
R2505	T10061	T10059	punct	/,Pygo2
R2506	T10071	T10070	punct	-,Pygo1
R2507	T10062	T10059	punct	-,Pygo2
R2508	T10072	T10070	punct	/,Pygo1
R2509	T10073	T10070	punct	-,Pygo1
R2510	T10168	T10166	prep	of,Loss
R2511	T10169	T10170	det	the,gene
R2512	T10074	T10070	punct	/,Pygo1
R2513	T10170	T10168	pobj	gene,of
R2514	T10171	T10170	compound	Pygo2,gene
R2515	T10172	T10166	punct	(,Loss
R2516	T10075	T10070	appos	Pygo2,Pygo1
R2517	T10173	T10166	appos	Pygo1,Loss
R2518	T10174	T10173	punct	+,Pygo1
R2519	T10076	T10075	punct	+,Pygo2
R2520	T10175	T10173	punct	/,Pygo1
R2521	T10176	T10173	punct	-,Pygo1
R2522	T10177	T10173	punct	/,Pygo1
R2523	T10077	T10075	punct	/,Pygo2
R2524	T10178	T10173	appos	Pygo2,Pygo1
R2525	T10179	T10178	punct	-,Pygo2
R2526	T10180	T10178	punct	/,Pygo2
R2527	T10181	T10178	punct	-,Pygo2
R2528	T10182	T10173	cc	and,Pygo1
R2529	T10078	T10075	punct	-,Pygo2
R2530	T10183	T10173	conj	Pygo1,Pygo1
R2531	T10184	T10183	punct	-,Pygo1
R2532	T10079	T10080	punct	(,middle
R2533	T10080	T10070	parataxis	middle,Pygo1
R2534	T10185	T10183	punct	/,Pygo1
R2535	T10186	T10183	punct	-,Pygo1
R2536	T10187	T10183	punct	/,Pygo1
R2537	T10081	T10082	compound	Figure,6O
R2538	T10188	T10183	appos	Pygo2,Pygo1
R2539	T10189	T10188	punct	-,Pygo2
R2540	T10082	T10080	dep	6O,middle
R2541	T10190	T10188	punct	/,Pygo2
R2542	T10191	T10188	punct	-,Pygo2
R2543	T10192	T10167	punct	),gave
R2544	T10083	T10080	punct	", ",middle
R2545	T10193	T10194	amod	greater,90
R2546	T10194	T10196	nummod	90,%
R2547	T10084	T10080	punct	),middle
R2548	T10195	T10194	quantmod	than,90
R2549	T10196	T10197	compound	%,reduction
R2550	T10197	T10167	dobj	reduction,gave
R2551	T10085	T10070	punct	", ",Pygo1
R2552	T10198	T10197	prep	in,reduction
R2553	T10199	T10200	compound	BAT,gal
R2554	T10200	T10202	compound	gal,expression
R2555	T10086	T10070	cc	and,Pygo1
R2556	T10201	T10200	punct	-,gal
R2557	T10202	T10198	pobj	expression,in
R2558	T10203	T10167	punct	.,gave
R2559	T10087	T10070	conj	Pygo1,Pygo1
R2560	T10205	T10206	nsubj	Loss,result
R2561	T10088	T10087	punct	-,Pygo1
R2562	T10207	T10205	prep	of,Loss
R2563	T10208	T10207	pobj	Pygo1,of
R2564	T10209	T10205	advmod	alone,Loss
R2565	T10089	T10087	punct	/,Pygo1
R2566	T10210	T10205	punct	(,Loss
R2567	T10211	T10205	appos	Pygo1,Loss
R2568	T10212	T10211	punct	-,Pygo1
R2569	T10090	T10087	punct	-,Pygo1
R2570	T10213	T10211	punct	/,Pygo1
R2571	T10214	T10211	punct	-,Pygo1
R2572	T10215	T10211	punct	/,Pygo1
R2573	T10216	T10211	appos	Pygo2,Pygo1
R2574	T10091	T10087	punct	/,Pygo1
R2575	T10217	T10216	punct	+,Pygo2
R2576	T10218	T10216	punct	/,Pygo2
R2577	T10219	T10216	punct	+,Pygo2
R2578	T10220	T10206	punct	),result
R2579	T10221	T10206	aux	did,result
R2580	T10222	T10206	neg	not,result
R2581	T10092	T10087	appos	Pygo2,Pygo1
R2582	T10223	T10206	prep	in,result
R2583	T10224	T10225	det	a,change
R2584	T10093	T10092	punct	-,Pygo2
R2585	T10225	T10223	pobj	change,in
R2586	T10226	T10225	amod	significant,change
R2587	T10227	T10206	punct	.,result
R2588	T10094	T10092	punct	/,Pygo2
R2589	T10229	T10230	advmod	Interestingly,showed
R2590	T10095	T10092	punct	-,Pygo2
R2591	T10231	T10230	punct	", ",showed
R2592	T10232	T10230	advmod	however,showed
R2593	T10233	T10230	punct	", ",showed
R2594	T10096	T10097	punct	(,right
R2595	T10234	T10235	det	the,Pygo1
R2596	T10235	T10230	nsubj	Pygo1,showed
R2597	T10236	T10235	punct	-,Pygo1
R2598	T10097	T10087	parataxis	right,Pygo1
R2599	T10237	T10235	punct	/,Pygo1
R2600	T10238	T10235	punct	-,Pygo1
R2601	T10239	T10235	punct	/,Pygo1
R2602	T10240	T10235	appos	Pygo2,Pygo1
R2603	T10098	T10099	compound	Figure,6O
R2604	T10241	T10240	punct	+,Pygo2
R2605	T10242	T10240	punct	/,Pygo2
R2606	T10243	T10240	punct	-,Pygo2
R2607	T10099	T10097	dep	6O,right
R2608	T10244	T10245	advmod	only,50
R2609	T10245	T10246	nummod	50,%
R2610	T10246	T10230	dobj	%,showed
R2611	T10100	T10097	punct	", ",right
R2612	T10247	T10246	prep	of,%
R2613	T10248	T10249	amod	wild,type
R2614	T10249	T10251	compound	type,expression
R2615	T10250	T10249	punct	-,type
R2616	T10101	T10097	punct	),right
R2617	T10251	T10247	pobj	expression,of
R2618	T10252	T10253	compound	BAT,gal
R2619	T10253	T10251	compound	gal,expression
R2620	T10102	T10054	compound	E18.5,kidneys
R2621	T10254	T10253	punct	-,gal
R2622	T10255	T10230	punct	", ",showed
R2623	T10256	T10230	advcl	suggesting,showed
R2624	T10103	T10104	det	a,role
R2625	T10257	T10258	det	a,contribution
R2626	T10258	T10256	dobj	contribution,suggesting
R2627	T10104	T10051	dobj	role,suggested
R2628	T10259	T10258	amod	minor,contribution
R2629	T10260	T10258	prep	by,contribution
R2630	T10261	T10260	pobj	Pygo1,by
R2631	T10262	T10258	prep	in,contribution
R2632	T10263	T10264	amod	canonical,signaling
R2633	T10264	T10262	pobj	signaling,in
R2634	T10265	T10264	compound	Wnt,signaling
R2635	T10105	T10104	amod	significant,role
R2636	T10266	T10230	punct	.,showed
R2637	T10268	T10269	mark	Although,decreased
R2638	T10106	T10104	prep	for,role
R2639	T10107	T10108	det	the,gene
R2640	T10269	T10275	advcl	decreased,revealed
R2641	T10270	T10271	compound	BAT,gal
R2642	T10108	T10106	pobj	gene,for
R2643	T10271	T10273	compound	gal,expression
R2644	T10272	T10271	punct	-,gal
R2645	T10109	T10108	compound	Pygo2,gene
R2646	T10110	T10104	prep	in,role
R2647	T10111	T10112	amod	canonical,signaling
R2648	T10273	T10269	nsubjpass	expression,decreased
R2649	T10112	T10110	pobj	signaling,in
R2650	T10274	T10269	auxpass	was,decreased
R2651	T10276	T10269	prep	in,decreased
R2652	T10277	T10278	det	the,kidney
R2653	T10113	T10112	compound	Wnt,signaling
R2654	T10278	T10276	pobj	kidney,in
R2655	T10279	T10278	nmod	E18.5,kidney
R2656	T10280	T10278	nmod	Pygo1,kidney
R2657	T10114	T10104	prep	in,role
R2658	T10281	T10280	punct	-,Pygo1
R2659	T10282	T10280	punct	/,Pygo1
R2660	T10283	T10280	punct	-,Pygo1
R2661	T10115	T10116	det	the,tree
R2662	T10284	T10280	punct	/,Pygo1
R2663	T10285	T10280	appos	Pygo2,Pygo1
R2664	T10116	T10114	pobj	tree,in
R2665	T10286	T10285	punct	+,Pygo2
R2666	T10287	T10285	punct	/,Pygo2
R2667	T10288	T10285	punct	-,Pygo2
R2668	T10117	T10116	amod	ureteric,tree
R2669	T10289	T10275	punct	", ",revealed
R2670	T10290	T10291	amod	confocal,analysis
R2671	T10291	T10275	nsubj	analysis,revealed
R2672	T10118	T10116	cc	and,tree
R2673	T10292	T10291	prep	of,analysis
R2674	T10293	T10292	pobj	kidneys,of
R2675	T10294	T10293	prep	with,kidneys
R2676	T10119	T10120	poss	its,derivatives
R2677	T10295	T10296	det	this,genotype
R2678	T10296	T10294	pobj	genotype,with
R2679	T10297	T10298	det	no,tips
R2680	T10120	T10116	conj	derivatives,tree
R2681	T10298	T10275	dobj	tips,revealed
R2682	T10299	T10298	amod	dilated,tips
R2683	T10300	T10298	amod	ureteric,tips
R2684	T10301	T10298	cc	or,tips
R2685	T10302	T10303	amod	significant,changes
R2686	T10303	T10298	conj	changes,tips
R2687	T10121	T10051	punct	.,suggested
R2688	T10304	T10303	prep	in,changes
R2689	T10305	T10306	amod	ureteric,tip
R2690	T10306	T10307	compound	tip,number
R2691	T10123	T10124	prep	In,performed
R2692	T10307	T10304	pobj	number,in
R2693	T10308	T10307	prep	per,number
R2694	T10309	T10308	pobj	area,per
R2695	T10310	T10303	prep	compared,changes
R2696	T10125	T10123	pobj	order,In
R2697	T10311	T10310	prep	with,compared
R2698	T10312	T10313	nmod	Pygo1,kidneys
R2699	T10313	T10311	pobj	kidneys,with
R2700	T10314	T10312	punct	-,Pygo1
R2701	T10126	T10127	aux	to,validate
R2702	T10315	T10312	punct	/,Pygo1
R2703	T10316	T10312	punct	-,Pygo1
R2704	T10317	T10312	punct	/,Pygo1
R2705	T10127	T10125	acl	validate,order
R2706	T10318	T10312	appos	Pygo2,Pygo1
R2707	T10319	T10318	punct	+,Pygo2
R2708	T10320	T10318	punct	/,Pygo2
R2709	T10128	T10127	cc	and,validate
R2710	T10321	T10318	punct	+,Pygo2
R2711	T10322	T10323	punct	(,shown
R2712	T10129	T10127	conj	quantify,validate
R2713	T10323	T10275	parataxis	shown,revealed
R2714	T10324	T10323	nsubj	data,shown
R2715	T10325	T10323	neg	not,shown
R2716	T10326	T10323	punct	),shown
R2717	T10130	T10131	det	the,changes
R2718	T10327	T10275	punct	.,revealed
R2719	T10131	T10129	dobj	changes,quantify
R2720	T10329	T10330	advmod	Collectively,suggest
R2721	T10331	T10330	punct	", ",suggest
R2722	T10132	T10133	compound	BAT,gal
R2723	T10332	T10333	det	these,results
R2724	T10333	T10330	nsubj	results,suggest
R2725	T10334	T10335	compound	BAT,gal
R2726	T10335	T10337	compound	gal,reporter
R2727	T10133	T10135	compound	gal,reporter
R2728	T10336	T10335	punct	-,gal
R2729	T10337	T10333	compound	reporter,results
R2730	T10338	T10339	det	a,role
R2731	T10134	T10133	punct	-,gal
R2732	T10339	T10330	dobj	role,suggest
R2733	T10340	T10339	amod	significant,role
R2734	T10341	T10339	prep	for,role
R2735	T10342	T10343	det	the,genes
R2736	T10343	T10341	pobj	genes,for
R2737	T10344	T10343	nmod	Pygo1,genes
R2738	T10135	T10136	compound	reporter,expression
R2739	T10345	T10344	cc	and,Pygo1
R2740	T10346	T10344	conj	Pygo2,Pygo1
R2741	T10347	T10339	prep	in,role
R2742	T10348	T10349	amod	canonical,signaling
R2743	T10136	T10131	compound	expression,changes
R2744	T10349	T10347	pobj	signaling,in
R2745	T10350	T10349	compound	Wnt,signaling
R2746	T10351	T10339	prep	during,role
R2747	T10137	T10131	prep	in,changes
R2748	T10352	T10351	pobj	development,during
R2749	T10353	T10352	prep	of,development
R2750	T10138	T10139	det	the,null
R2751	T10354	T10355	det	the,tree
R2752	T10139	T10137	pobj	null,in
R2753	T10140	T10139	compound	Pygo2,null
R2754	T10141	T10139	cc	and,null
R2755	T10355	T10353	pobj	tree,of
R2756	T10142	T10143	compound	Pygo1,Pygo2
R2757	T10356	T10355	amod	ureteric,tree
R2758	T10357	T10355	prep	of,tree
R2759	T10143	T10145	compound	Pygo2,nulls
R2760	T10358	T10359	det	the,kidney
R2761	T10359	T10357	pobj	kidney,of
R2762	T10360	T10330	punct	.,suggest
R2763	T10144	T10143	punct	/,Pygo2
R2764	T10145	T10139	conj	nulls,null
R2765	T10146	T10124	punct	", ",performed
R2766	T10147	T10124	nsubj	we,performed
R2767	T10148	T10149	compound	ELISA,measurements
R2768	T10149	T10124	dobj	measurements,performed
R2769	T10150	T10149	prep	of,measurements
R2770	T10151	T10152	npadvmod	transgene,specific
R2771	T10152	T10153	amod	specific,levels
R2772	T10362	T10363	advmod	Interestingly,showed
R2773	T10153	T10150	pobj	levels,of
R2774	T10364	T10363	punct	", ",showed
R2775	T10154	T10155	compound	β,galactosidase
R2776	T10365	T10363	advmod	however,showed
R2777	T10366	T10363	punct	", ",showed
R2778	T10155	T10153	compound	galactosidase,levels
R2779	T10367	T10368	advmod	even,in
R2780	T10368	T10363	prep	in,showed
R2781	T10369	T10370	amod	wild,type
R2782	T10156	T10155	punct	-,galactosidase
R2783	T10370	T10372	compound	type,mice
R2784	T10371	T10370	punct	-,type
R2785	T10372	T10368	pobj	mice,in
R2786	T10373	T10374	det	the,reporter
R2787	T10374	T10363	nsubj	reporter,showed
R2788	T10157	T10149	prep	in,measurements
R2789	T10375	T10376	compound	BAT,gal
R2790	T10376	T10374	compound	gal,reporter
R2791	T10377	T10376	punct	-,gal
R2792	T10378	T10379	det	no,expression
R2793	T10158	T10159	compound	E18.5,kidneys
R2794	T10379	T10363	dobj	expression,showed
R2795	T10380	T10379	prep	in,expression
R2796	T10159	T10157	pobj	kidneys,in
R2797	T10381	T10382	det	the,mesenchyme
R2798	T10382	T10380	pobj	mesenchyme,in
R2799	T10383	T10382	amod	developing,mesenchyme
R2800	T10160	T10161	punct	(,Figure
R2801	T10384	T10382	amod	metanephric,mesenchyme
R2802	T10385	T10382	punct	", ",mesenchyme
R2803	T10386	T10382	cc	or,mesenchyme
R2804	T10161	T10124	parataxis	Figure,performed
R2805	T10387	T10388	amod	metanephric,mesenchyme
R2806	T10388	T10389	npadvmod	mesenchyme,derived
R2807	T10389	T10391	amod	derived,structures
R2808	T10162	T10161	nummod	7,Figure
R2809	T10390	T10389	punct	-,derived
R2810	T10391	T10382	conj	structures,mesenchyme
R2811	T10163	T10161	punct	),Figure
R2812	T10392	T10391	punct	", ",structures
R2813	T10393	T10394	amod	such,as
R2814	T10394	T10391	prep	as,structures
R2815	T10395	T10396	amod	renal,vesicles
R2816	T10164	T10124	punct	.,performed
R2817	T10396	T10394	pobj	vesicles,as
R2818	T10397	T10396	punct	", ",vesicles
R2819	T10166	T10167	nsubj	Loss,gave
R2820	T10398	T10399	npadvmod	S,shaped
R2821	T10399	T10401	amod	shaped,bodies
R2822	T10400	T10399	punct	-,shaped
R2823	T10401	T10396	conj	bodies,vesicles
R2824	T10402	T10401	punct	", ",bodies
R2825	T10460	T10461	amod	ureteric,bud
R2826	T10403	T10401	conj	tubules,bodies
R2827	T10404	T10403	punct	", ",tubules
R2828	T10405	T10403	cc	and,tubules
R2829	T10406	T10403	conj	glomeruli,tubules
R2830	T10461	T10462	compound	bud,expression
R2831	T10407	T10363	punct	.,showed
R2832	T10409	T10410	nsubjpass	This,reported
R2833	T10462	T10457	nsubj	expression,rescue
R2834	T10411	T10410	aux	has,reported
R2835	T10412	T10410	auxpass	been,reported
R2836	T10463	T10462	prep	of,expression
R2837	T10413	T10410	advmod	previously,reported
R2838	T10414	T10410	punct	", ",reported
R2839	T10415	T10410	cc	and,reported
R2840	T10416	T10417	auxpass	was,interpreted
R2841	T10417	T10410	conj	interpreted,reported
R2842	T10464	T10463	pobj	Wnt1,of
R2843	T10418	T10419	aux	to,indicate
R2844	T10419	T10417	xcomp	indicate,interpreted
R2845	T10420	T10421	det	the,absence
R2846	T10465	T10462	punct	", ",expression
R2847	T10421	T10419	dobj	absence,indicate
R2848	T10422	T10421	prep	of,absence
R2849	T10423	T10424	amod	canonical,signaling
R2850	T10424	T10422	pobj	signaling,of
R2851	T10466	T10462	acl	thought,expression
R2852	T10425	T10424	compound	Wnt,signaling
R2853	T10426	T10421	prep	in,absence
R2854	T10467	T10468	aux	to,act
R2855	T10427	T10428	det	the,mesenchyme
R2856	T10428	T10426	pobj	mesenchyme,in
R2857	T10429	T10428	amod	metanephric,mesenchyme
R2858	T10430	T10431	punct	[,21
R2859	T10468	T10466	xcomp	act,thought
R2860	T10431	T10419	parataxis	21,indicate
R2861	T10432	T10431	punct	],21
R2862	T10469	T10468	prep	through,act
R2863	T10433	T10410	punct	.,reported
R2864	T10435	T10436	nsubj	Results,argue
R2865	T10470	T10471	amod	canonical,signaling
R2866	T10437	T10435	prep	from,Results
R2867	T10471	T10469	pobj	signaling,through
R2868	T10438	T10439	amod	other,studies
R2869	T10439	T10437	pobj	studies,from
R2870	T10440	T10436	punct	", ",argue
R2871	T10441	T10436	advmod	however,argue
R2872	T10472	T10471	compound	Wnt,signaling
R2873	T10442	T10436	punct	", ",argue
R2874	T10443	T10436	prep	for,argue
R2875	T10444	T10445	det	the,presence
R2876	T10473	T10457	punct	", ",rescue
R2877	T10445	T10443	pobj	presence,for
R2878	T10446	T10445	prep	of,presence
R2879	T10447	T10448	amod	canonical,signaling
R2880	T10474	T10457	aux	can,rescue
R2881	T10448	T10446	pobj	signaling,of
R2882	T10449	T10448	compound	Wnt,signaling
R2883	T10450	T10445	prep	in,presence
R2884	T10451	T10452	det	the,mesenchyme
R2885	T10475	T10476	compound	Wnt9b,mutants
R2886	T10452	T10450	pobj	mesenchyme,in
R2887	T10453	T10452	amod	metanephric,mesenchyme
R2888	T10454	T10436	punct	.,argue
R2889	T10476	T10457	dobj	mutants,rescue
R2890	T10456	T10457	prep	For,rescue
R2891	T10458	T10456	pobj	example,For
R2892	T10459	T10457	punct	", ",rescue
R2893	T10477	T10457	cc	and,rescue
R2894	T10478	T10457	conj	induce,rescue
R2895	T10479	T10478	dobj	nephrogenesis,induce
R2896	T10566	T10567	mark	as,expected
R2897	T10480	T10479	prep	of,nephrogenesis
R2898	T10567	T10557	advcl	expected,undergo
R2899	T10568	T10557	punct	", ",undergo
R2900	T10481	T10482	det	the,mesenchyme
R2901	T10569	T10557	cc	but,undergo
R2902	T10570	T10557	conj	failed,undergo
R2903	T10482	T10480	pobj	mesenchyme,of
R2904	T10571	T10572	aux	to,show
R2905	T10572	T10570	xcomp	show,failed
R2906	T10573	T10574	compound	BAT,gal
R2907	T10483	T10482	amod	metanephric,mesenchyme
R2908	T10574	T10576	compound	gal,expression
R2909	T10575	T10574	punct	-,gal
R2910	T10576	T10572	dobj	expression,show
R2911	T10484	T10485	punct	[,2
R2912	T10485	T10478	parataxis	2,induce
R2913	T10577	T10578	punct	(,shown
R2914	T10578	T10572	parataxis	shown,show
R2915	T10486	T10485	punct	],2
R2916	T10579	T10578	nsubj	data,shown
R2917	T10580	T10578	neg	not,shown
R2918	T10581	T10578	punct	),shown
R2919	T10487	T10457	punct	.,rescue
R2920	T10582	T10570	punct	", ",failed
R2921	T10583	T10570	advcl	suggesting,failed
R2922	T10584	T10585	mark	that,is
R2923	T10489	T10490	nsubj	This,suggests
R2924	T10585	T10583	ccomp	is,suggesting
R2925	T10586	T10587	det	this,transgene
R2926	T10587	T10585	nsubj	transgene,is
R2927	T10491	T10492	mark	that,report
R2928	T10588	T10585	neg	not,is
R2929	T10589	T10590	det	an,reporter
R2930	T10590	T10585	attr	reporter,is
R2931	T10591	T10590	amod	accurate,reporter
R2932	T10492	T10490	ccomp	report,suggests
R2933	T10592	T10590	prep	of,reporter
R2934	T10593	T10594	amod	canonical,signaling
R2935	T10594	T10592	pobj	signaling,of
R2936	T10493	T10494	det	the,transgene
R2937	T10595	T10594	compound	Wnt,signaling
R2938	T10596	T10590	prep	in,reporter
R2939	T10597	T10598	det	the,mesenchyme
R2940	T10494	T10492	nsubj	transgene,report
R2941	T10598	T10596	pobj	mesenchyme,in
R2942	T10599	T10598	amod	metanephric,mesenchyme
R2943	T10600	T10598	prep	of,mesenchyme
R2944	T10601	T10602	det	the,kidney
R2945	T10602	T10600	pobj	kidney,of
R2946	T10603	T10602	amod	developing,kidney
R2947	T10495	T10496	compound	BAT,gal
R2948	T10604	T10555	punct	.,observed
R2949	T10496	T10494	compound	gal,transgene
R2950	T10497	T10496	punct	-,gal
R2951	T10498	T10492	aux	might,report
R2952	T10499	T10492	neg	not,report
R2953	T10500	T10492	advmod	accurately,report
R2954	T10501	T10502	amod	canonical,signaling
R2955	T10502	T10492	dobj	signaling,report
R2956	T10503	T10502	compound	Wnt,signaling
R2957	T10504	T10492	prep	in,report
R2958	T10505	T10506	det	the,mesenchyme
R2959	T10506	T10504	pobj	mesenchyme,in
R2960	T10507	T10506	amod	metanephric,mesenchyme
R2961	T10508	T10490	punct	.,suggests
R2962	T10510	T10511	aux	To,address
R2963	T10511	T10512	advcl	address,incubated
R2964	T10513	T10514	det	this,question
R2965	T10514	T10511	dobj	question,address
R2966	T10515	T10512	punct	", ",incubated
R2967	T10516	T10512	nsubj	we,incubated
R2968	T10517	T10518	nmod	E11.5,mesenchyme
R2969	T10518	T10512	dobj	mesenchyme,incubated
R2970	T10519	T10518	amod	metanephric,mesenchyme
R2971	T10520	T10512	prep	in,incubated
R2972	T10521	T10522	compound	lithium,chloride
R2973	T10522	T10520	pobj	chloride,in
R2974	T10523	T10522	punct	(,chloride
R2975	T10524	T10522	appos	LiCl,chloride
R2976	T10525	T10522	punct	),chloride
R2977	T10526	T10522	punct	", ",chloride
R2978	T10527	T10528	dep	which,activates
R2979	T10528	T10522	relcl	activates,chloride
R2980	T10529	T10530	amod	canonical,signaling
R2981	T10530	T10528	dobj	signaling,activates
R2982	T10531	T10530	compound	Wnt,signaling
R2983	T10532	T10528	prep	through,activates
R2984	T10533	T10532	pobj	inhibition,through
R2985	T10534	T10533	prep	of,inhibition
R2986	T10535	T10534	pobj	GSK3,of
R2987	T10536	T10528	punct	", ",activates
R2988	T10537	T10528	cc	and,activates
R2989	T10538	T10539	advmod	also,functions
R2990	T10539	T10528	conj	functions,activates
R2991	T10540	T10539	prep	as,functions
R2992	T10541	T10542	det	an,inducer
R2993	T10542	T10540	pobj	inducer,as
R2994	T10543	T10542	prep	of,inducer
R2995	T10544	T10543	pobj	nephrogenesis,of
R2996	T10545	T10542	prep	in,inducer
R2997	T10546	T10547	compound	kidney,organ
R2998	T10547	T10548	compound	organ,culture
R2999	T10548	T10545	pobj	culture,in
R3000	T10549	T10550	punct	[,22
R3001	T10550	T10539	parataxis	22,functions
R3002	T10551	T10550	punct	],22
R3003	T10552	T10512	punct	.,incubated
R3004	T10554	T10555	nsubj	We,observed
R3005	T10556	T10557	mark	that,undergo
R3006	T10557	T10555	ccomp	undergo,observed
R3007	T10558	T10559	npadvmod	LiCl,treated
R3008	T10559	T10561	amod	treated,mesenchyme
R3009	T10560	T10559	punct	-,treated
R3010	T10561	T10557	nsubj	mesenchyme,undergo
R3011	T10562	T10561	amod	metanephric,mesenchyme
R3012	T10563	T10557	aux	did,undergo
R3013	T10564	T10557	dobj	nephrogenesis,undergo
R3014	T10565	T10557	punct	", ",undergo
R6861	T24238	T24239	amod	Reduced,signaling
R6862	T24240	T24239	amod	canonical,signaling
R6863	T24241	T24239	compound	Wnt,signaling
R6864	T24242	T24239	prep	in,signaling
R6865	T24243	T24244	nmod	Pygo2,mutant
R6866	T24244	T24249	compound	mutant,embryos
R6867	T24245	T24243	cc	and,Pygo2
R6868	T24246	T24247	compound	Pygo1,Pygo2
R6869	T24247	T24243	conj	Pygo2,Pygo2
R6870	T24248	T24247	punct	/,Pygo2
R6871	T24249	T24242	pobj	embryos,in
R6872	T24250	T24239	punct	.,signaling
R6873	T24252	T24253	compound	E10.5,embryos
R6874	T24254	T24253	punct	", ",embryos
R6875	T24255	T24253	appos	all,embryos
R6876	T24256	T24255	prep	with,all
R6877	T24257	T24258	det	the,reporter
R6878	T24258	T24256	pobj	reporter,with
R6879	T24259	T24260	compound	BAT,gal
R6880	T24260	T24262	compound	gal,transgene
R6881	T24261	T24260	punct	-,gal
R6882	T24262	T24258	compound	transgene,reporter
R6883	T24263	T24258	prep	of,reporter
R6884	T24264	T24265	amod	canonical,signaling
R6885	T24265	T24263	pobj	signaling,of
R6886	T24266	T24265	compound	Wnt,signaling
R6887	T24267	T24253	punct	.,embryos
R6888	T24269	T24270	punct	(,A
R6889	T24270	T24271	meta	A,showed
R6890	T24272	T24270	punct	),A
R6891	T24273	T24274	nmod	Pygo1,embryos
R6892	T24274	T24271	nsubj	embryos,showed
R6893	T24275	T24273	punct	+,Pygo1
R6894	T24276	T24273	punct	/,Pygo1
R6895	T24277	T24273	punct	+,Pygo1
R6896	T24278	T24273	punct	/,Pygo1
R6897	T24279	T24273	appos	Pygo2,Pygo1
R6898	T24280	T24279	punct	+,Pygo2
R6899	T24281	T24279	punct	/,Pygo2
R6900	T24282	T24279	punct	-,Pygo2
R6901	T24283	T24284	amod	normal,staining
R6902	T24284	T24271	dobj	staining,showed
R6903	T24285	T24286	compound	X,gal
R6904	T24286	T24284	compound	gal,staining
R6905	T24287	T24286	punct	-,gal
R6906	T24288	T24271	prep	in,showed
R6907	T24289	T24290	det	the,brain
R6908	T24290	T24288	pobj	brain,in
R6909	T24291	T24290	amod	developing,brain
R6910	T24292	T24290	punct	", ",brain
R6911	T24293	T24294	amod	pharyngeal,pouches
R6912	T24294	T24290	appos	pouches,brain
R6913	T24295	T24290	punct	", ",brain
R6914	T24296	T24297	amod	otic,vesicle
R6915	T24297	T24290	appos	vesicle,brain
R6916	T24298	T24290	punct	", ",brain
R6917	T24299	T24300	amod	apical,ridges
R6918	T24300	T24290	appos	ridges,brain
R6919	T24301	T24300	amod	ectodermal,ridges
R6920	T24302	T24300	prep	of,ridges
R6921	T24303	T24304	det	the,buds
R6922	T24304	T24302	pobj	buds,of
R6923	T24305	T24306	amod	fore,limb
R6924	T24306	T24304	compound	limb,buds
R6925	T24307	T24305	cc	and,fore
R6926	T24308	T24305	conj	hind,fore
R6927	T24309	T24288	punct	", ",in
R6928	T24310	T24288	cc	and,in
R6929	T24311	T24288	conj	in,in
R6930	T24312	T24311	pobj	somites,in
R6931	T24313	T24271	punct	.,showed
R6932	T24315	T24316	punct	(,B
R6933	T24316	T24317	meta	B,showed
R6934	T24318	T24316	punct	),B
R6935	T24319	T24320	nmod	Pygo1,embryos
R6936	T24320	T24317	nsubj	embryos,showed
R6937	T24321	T24319	punct	+,Pygo1
R6938	T24322	T24319	punct	/,Pygo1
R6939	T24323	T24319	punct	-,Pygo1
R6940	T24324	T24319	punct	/,Pygo1
R6941	T24325	T24319	appos	Pygo2,Pygo1
R6942	T24326	T24325	punct	-,Pygo2
R6943	T24327	T24325	punct	/,Pygo2
R6944	T24328	T24325	punct	-,Pygo2
R6945	T24329	T24320	punct	", ",embryos
R6946	T24330	T24320	prep	with,embryos
R6947	T24331	T24330	pobj	loss,with
R6948	T24332	T24331	prep	of,loss
R6949	T24333	T24334	det	both,alleles
R6950	T24334	T24332	pobj	alleles,of
R6951	T24335	T24334	compound	Pygo2,alleles
R6952	T24336	T24317	punct	", ",showed
R6953	T24337	T24338	amod	reduced,reporter
R6954	T24338	T24345	compound	reporter,expression
R6955	T24339	T24337	cc	but,reduced
R6956	T24340	T24339	neg	not,but
R6957	T24341	T24337	conj	absent,reduced
R6958	T24342	T24343	compound	BAT,gal
R6959	T24343	T24338	compound	gal,reporter
R6960	T24344	T24343	punct	-,gal
R6961	T24345	T24317	dobj	expression,showed
R6962	T24346	T24317	prep	in,showed
R6963	T24347	T24348	amod	many,structures
R6964	T24348	T24346	pobj	structures,in
R6965	T24349	T24348	amod	developing,structures
R6966	T24350	T24348	punct	", ",structures
R6967	T24351	T24348	prep	including,structures
R6968	T24352	T24353	amod	pharyngeal,pouches
R6969	T24353	T24351	pobj	pouches,including
R6970	T24354	T24353	punct	", ",pouches
R6971	T24355	T24356	amod	otic,vesicle
R6972	T24356	T24353	conj	vesicle,pouches
R6973	T24357	T24356	punct	", ",vesicle
R6974	T24358	T24356	cc	and,vesicle
R6975	T24359	T24356	conj	somites,vesicle
R6976	T24360	T24317	punct	.,showed
R6977	T24362	T24363	punct	(,C
R6978	T24363	T24364	meta	C,showed
R6979	T24365	T24363	punct	),C
R6980	T24366	T24367	nmod	Pygo1,embryo
R6981	T24367	T24364	nsubj	embryo,showed
R6982	T24368	T24366	punct	-,Pygo1
R6983	T24369	T24366	punct	/,Pygo1
R6984	T24370	T24366	punct	-,Pygo1
R6985	T24371	T24366	punct	/,Pygo1
R6986	T24372	T24366	appos	Pygo2,Pygo1
R6987	T24373	T24372	punct	+,Pygo2
R6988	T24374	T24372	punct	/,Pygo2
R6989	T24375	T24372	punct	-,Pygo2
R6990	T24376	T24367	punct	", ",embryo
R6991	T24377	T24367	prep	with,embryo
R6992	T24378	T24377	pobj	mutation,with
R6993	T24379	T24378	prep	of,mutation
R6994	T24380	T24381	det	both,alleles
R6995	T24381	T24379	pobj	alleles,of
R6996	T24382	T24381	compound	Pygo1,alleles
R6997	T24383	T24381	punct	", ",alleles
R6998	T24384	T24381	cc	but,alleles
R6999	T24385	T24386	nummod	one,allele
R7000	T24386	T24381	conj	allele,alleles
R7001	T24387	T24388	amod	wild,type
R7002	T24388	T24386	compound	type,allele
R7003	T24389	T24388	punct	-,type
R7004	T24390	T24386	compound	Pygo2,allele
R7005	T24391	T24364	punct	", ",showed
R7006	T24392	T24393	amod	normal,expression
R7007	T24393	T24364	dobj	expression,showed
R7008	T24394	T24395	compound	BAT,gal
R7009	T24395	T24393	compound	gal,expression
R7010	T24396	T24395	punct	-,gal
R7011	T24397	T24364	punct	.,showed
R7012	T24399	T24400	punct	(,D
R7013	T24400	T24401	meta	D,showed
R7014	T24402	T24400	punct	),D
R7015	T24403	T24404	amod	Double,homozygous
R7016	T24404	T24406	amod	homozygous,embryos
R7017	T24405	T24404	punct	-,homozygous
R7018	T24406	T24401	nsubj	embryos,showed
R7019	T24407	T24406	nmod	mutant,embryos
R7020	T24408	T24406	nmod	Pygo1,embryos
R7021	T24409	T24408	punct	-,Pygo1
R7022	T24410	T24408	punct	/,Pygo1
R7023	T24411	T24408	punct	-,Pygo1
R7024	T24412	T24408	punct	/,Pygo1
R7025	T24413	T24408	appos	Pygo2,Pygo1
R7026	T24414	T24413	punct	-,Pygo2
R7027	T24415	T24413	punct	/,Pygo2
R7028	T24416	T24413	punct	-,Pygo2
R7029	T24417	T24401	advmod	still,showed
R7030	T24418	T24419	det	some,expression
R7031	T24419	T24401	dobj	expression,showed
R7032	T24420	T24419	amod	remaining,expression
R7033	T24421	T24422	compound	BAT,gal
R7034	T24422	T24419	compound	gal,expression
R7035	T24423	T24422	punct	-,gal
R7036	T24424	T24401	punct	", ",showed
R7037	T24425	T24401	advcl	suggesting,showed
R7038	T24426	T24427	amod	residual,signaling
R7039	T24427	T24425	dobj	signaling,suggesting
R7040	T24428	T24427	amod	canonical,signaling
R7041	T24429	T24427	compound	Wnt,signaling
R7042	T24430	T24401	punct	.,showed
R7043	T24432	T24433	nsubj	Embryos,were
R7044	T24434	T24432	prep	in,Embryos
R7045	T24435	T24436	nmod	panels,A
R7046	T24436	T24434	pobj	A,in
R7047	T24437	T24436	punct	(,A
R7048	T24438	T24436	punct	),A
R7049	T24439	T24436	cc	and,A
R7050	T24440	T24441	punct	(,B
R7051	T24441	T24436	conj	B,A
R7052	T24442	T24433	punct	),were
R7053	T24443	T24433	prep	from,were
R7054	T24444	T24445	det	the,litter
R7055	T24445	T24443	pobj	litter,from
R7056	T24446	T24445	amod	same,litter
R7057	T24447	T24433	cc	and,were
R7058	T24448	T24449	auxpass	were,processed
R7059	T24449	T24433	conj	processed,were
R7060	T24450	T24449	prep	in,processed
R7061	T24451	T24450	pobj	parallel,in
R7062	T24452	T24449	punct	", ",processed
R7063	T24453	T24454	mark	while,were
R7064	T24454	T24449	advcl	were,processed
R7065	T24455	T24454	nsubj	embryos,were
R7066	T24456	T24455	prep	in,embryos
R7067	T24457	T24456	punct	(,in
R7068	T24458	T24456	pobj	C,in
R7069	T24459	T24458	punct	),C
R7070	T24460	T24458	cc	and,C
R7071	T24461	T24462	punct	(,D
R7072	T24462	T24458	conj	D,C
R7073	T24463	T24454	punct	),were
R7074	T24464	T24454	prep	from,were
R7075	T24465	T24466	det	a,litter
R7076	T24466	T24464	pobj	litter,from
R7077	T24467	T24466	amod	separate,litter
R7078	T24468	T24466	punct	", ",litter
R7079	T24469	T24470	advmod	also,processed
R7080	T24470	T24466	acl	processed,litter
R7081	T24471	T24470	prep	in,processed
R7082	T24472	T24471	pobj	parallel,in
R7083	T24473	T24454	punct	", ",were
R7084	T24474	T24454	cc	and,were
R7085	T24475	T24454	conj	were,were
R7086	T24476	T24477	advmod	slightly,more
R7087	T24477	T24478	advmod	more,advanced
R7088	T24478	T24475	acomp	advanced,were
R7089	T24479	T24478	advmod	developmentally,advanced
R7090	T24480	T24433	punct	.,were
R7091	T24482	T24483	amod	Original,magnification
R7092	T24483	T24484	dep	magnification,20
R7093	T24485	T24486	punct	(,B
R7094	T24486	T24484	parataxis	B,20
R7095	T24487	T24486	nmod	A,B
R7096	T24488	T24486	punct	", ",B
R7097	T24489	T24486	punct	),B
R7098	T24490	T24484	punct	×,20
R7099	T24491	T24484	punct	;,20
R7100	T24492	T24493	punct	(,D
R7101	T24493	T24496	parataxis	D,16
R7102	T24494	T24493	nmod	C,D
R7103	T24495	T24493	punct	", ",D
R7104	T24496	T24484	appos	16,20
R7105	T24497	T24493	punct	),D
R7106	T24498	T24496	punct	×,16
R7107	T24499	T24484	punct	.,20
R7108	T25934	T25935	nsubjpass	Pygo2,required
R7109	T25936	T25935	auxpass	is,required
R7110	T25937	T25935	prep	for,required
R7111	T25938	T25939	compound	BAT,gal
R7112	T25939	T25941	compound	gal,reporter
R7113	T25940	T25939	punct	-,gal
R7114	T25941	T25942	compound	reporter,expression
R7115	T25942	T25937	pobj	expression,for
R7116	T25943	T25935	prep	in,required
R7117	T25944	T25945	amod	ureteric,bud
R7118	T25945	T25946	npadvmod	bud,derived
R7119	T25946	T25948	amod	derived,structures
R7120	T25947	T25946	punct	-,derived
R7121	T25948	T25943	pobj	structures,in
R7122	T25949	T25948	prep	of,structures
R7123	T25950	T25951	det	the,kidney
R7124	T25951	T25949	pobj	kidney,of
R7125	T25952	T25951	amod	developing,kidney
R7126	T25953	T25935	punct	.,required
R7127	T25955	T25956	compound	X,Gal
R7128	T25956	T25958	compound	Gal,staining
R7129	T25957	T25956	punct	-,Gal
R7130	T25959	T25958	prep	of,staining
R7131	T25960	T25961	compound	BAT,gal
R7132	T25961	T25963	npadvmod	gal,transgenic
R7133	T25962	T25961	punct	-,gal
R7134	T25963	T25964	amod	transgenic,tracts
R7135	T25964	T25959	pobj	tracts,of
R7136	T25965	T25966	punct	(,A
R7137	T25966	T25967	parataxis	A,E10.5
R7138	T25967	T25964	nmod	E10.5,tracts
R7139	T25968	T25969	punct	-,C
R7140	T25969	T25966	prep	C,A
R7141	T25970	T25966	punct	),A
R7142	T25971	T25967	punct	", ",E10.5
R7143	T25972	T25967	cc	and,E10.5
R7144	T25973	T25974	punct	(,E
R7145	T25974	T25975	parataxis	E,E13.5
R7146	T25975	T25967	conj	E13.5,E10.5
R7147	T25976	T25977	punct	-,L
R7148	T25977	T25974	prep	L,E
R7149	T25978	T25974	punct	),E
R7150	T25979	T25964	amod	urogenital,tracts
R7151	T25980	T25964	punct	", ",tracts
R7152	T25981	T25964	cc	and,tracts
R7153	T25982	T25983	punct	(,M
R7154	T25983	T25984	parataxis	M,kidneys
R7155	T25984	T25964	conj	kidneys,tracts
R7156	T25985	T25986	punct	-,O
R7157	T25986	T25983	prep	O,M
R7158	T25987	T25983	punct	),M
R7159	T25988	T25984	compound	E18.5,kidneys
R7160	T25989	T25958	punct	.,staining
R7161	T25991	T25992	punct	(,A
R7162	T25992	T25993	meta	A,Pygo1
R7163	T25994	T25992	punct	),A
R7164	T25995	T25993	punct	-,Pygo1
R7165	T25996	T25993	punct	/,Pygo1
R7166	T25997	T25993	punct	-,Pygo1
R7167	T25998	T25993	punct	/,Pygo1
R7168	T25999	T25993	appos	Pygo2,Pygo1
R7169	T26000	T25999	punct	+,Pygo2
R7170	T26001	T25999	punct	/,Pygo2
R7171	T26002	T25999	punct	+,Pygo2
R7172	T26003	T26004	punct	(,left
R7173	T26004	T25993	parataxis	left,Pygo1
R7174	T26005	T26004	punct	),left
R7175	T26006	T25993	cc	and,Pygo1
R7176	T26007	T25993	conj	Pygo1,Pygo1
R7177	T26008	T26007	punct	+,Pygo1
R7178	T26009	T26007	punct	/,Pygo1
R7179	T26010	T26007	punct	-,Pygo1
R7180	T26011	T26007	punct	/,Pygo1
R7181	T26012	T26007	appos	Pygo2,Pygo1
R7182	T26013	T26012	punct	-,Pygo2
R7183	T26014	T26012	punct	/,Pygo2
R7184	T26015	T26012	punct	-,Pygo2
R7185	T26016	T26017	punct	(,right
R7186	T26017	T26007	parataxis	right,Pygo1
R7187	T26018	T26017	punct	),right
R7188	T26019	T26007	punct	.,Pygo1
R7189	T26022	T26023	det	the,loss
R7190	T26023	T26021	dobj	loss,Note
R7191	T26024	T26023	prep	of,loss
R7192	T26025	T26026	compound	reporter,activity
R7193	T26026	T26024	pobj	activity,of
R7194	T26027	T26023	prep	in,loss
R7195	T26028	T26029	det	the,duct
R7196	T26029	T26027	pobj	duct,in
R7197	T26030	T26029	amod	nephric,duct
R7198	T26031	T26032	punct	(,arrowhead
R7199	T26032	T26023	parataxis	arrowhead,loss
R7200	T26033	T26032	amod	black,arrowhead
R7201	T26034	T26032	punct	),arrowhead
R7202	T26035	T26023	cc	and,loss
R7203	T26036	T26023	conj	reduction,loss
R7204	T26037	T26036	prep	of,reduction
R7205	T26038	T26037	pobj	staining,of
R7206	T26039	T26036	prep	in,reduction
R7207	T26040	T26041	det	the,bud
R7208	T26041	T26039	pobj	bud,in
R7209	T26042	T26041	amod	ureteric,bud
R7210	T26043	T26044	punct	(,arrow
R7211	T26044	T26041	parataxis	arrow,bud
R7212	T26045	T26044	amod	white,arrow
R7213	T26046	T26044	punct	),arrow
R7214	T26047	T26041	prep	of,bud
R7215	T26048	T26049	det	the,embryo
R7216	T26049	T26047	pobj	embryo,of
R7217	T26050	T26051	npadvmod	Pygo2,null
R7218	T26051	T26049	amod	null,embryo
R7219	T26052	T26053	punct	(,Right
R7220	T26053	T26036	parataxis	Right,reduction
R7221	T26054	T26053	punct	),Right
R7222	T26055	T26021	punct	.,Note
R7223	T26057	T26058	punct	(,B
R7224	T26058	T26059	meta	B,embryo
R7225	T26060	T26058	punct	),B
R7226	T26061	T26059	nmod	Pygo1,embryo
R7227	T26062	T26061	punct	-,Pygo1
R7228	T26063	T26061	punct	/,Pygo1
R7229	T26064	T26061	punct	-,Pygo1
R7230	T26065	T26061	punct	/,Pygo1
R7231	T26066	T26061	appos	Pygo2,Pygo1
R7232	T26067	T26066	punct	+,Pygo2
R7233	T26068	T26066	punct	/,Pygo2
R7234	T26069	T26066	punct	-,Pygo2
R7235	T26070	T26059	compound	E10.5,embryo
R7236	T26071	T26059	prep	with,embryo
R7237	T26072	T26073	compound	BAT,gal
R7238	T26073	T26075	compound	gal,reporter
R7239	T26074	T26073	punct	-,gal
R7240	T26075	T26076	compound	reporter,activity
R7241	T26076	T26071	pobj	activity,with
R7242	T26077	T26076	prep	in,activity
R7243	T26078	T26079	det	the,duct
R7244	T26079	T26077	pobj	duct,in
R7245	T26080	T26079	amod	nephric,duct
R7246	T26081	T26082	punct	(,arrowhead
R7247	T26082	T26079	parataxis	arrowhead,duct
R7248	T26083	T26082	amod	black,arrowhead
R7249	T26084	T26082	punct	),arrowhead
R7250	T26085	T26079	cc	and,duct
R7251	T26086	T26087	amod	ureteric,bud
R7252	T26087	T26079	conj	bud,duct
R7253	T26088	T26089	punct	(,arrow
R7254	T26089	T26087	parataxis	arrow,bud
R7255	T26090	T26089	amod	white,arrow
R7256	T26091	T26089	punct	),arrow
R7257	T26092	T26059	punct	.,embryo
R7258	T26094	T26095	punct	(,C
R7259	T26095	T26096	meta	C,embryo
R7260	T26097	T26095	punct	),C
R7261	T26098	T26096	nmod	Pygo1,embryo
R7262	T26099	T26098	punct	-,Pygo1
R7263	T26100	T26098	punct	/,Pygo1
R7264	T26101	T26098	punct	-,Pygo1
R7265	T26102	T26098	punct	/,Pygo1
R7266	T26103	T26098	appos	Pygo,Pygo1
R7267	T26104	T26103	punct	-,Pygo
R7268	T26105	T26103	punct	/,Pygo
R7269	T26106	T26103	punct	-,Pygo
R7270	T26107	T26096	punct	", ",embryo
R7271	T26108	T26109	mark	with,lost
R7272	T26109	T26096	advcl	lost,embryo
R7273	T26110	T26111	compound	reporter,expression
R7274	T26111	T26109	nsubj	expression,lost
R7275	T26112	T26109	prep	in,lost
R7276	T26113	T26114	det	the,duct
R7277	T26114	T26112	pobj	duct,in
R7278	T26115	T26114	amod	nephric,duct
R7279	T26116	T26109	cc	and,lost
R7280	T26117	T26109	conj	reduced,lost
R7281	T26118	T26117	prep	in,reduced
R7282	T26119	T26120	det	the,bud
R7283	T26120	T26118	pobj	bud,in
R7284	T26121	T26120	amod	ureteric,bud
R7285	T26122	T26123	punct	(,arrow
R7286	T26123	T26109	parataxis	arrow,lost
R7287	T26124	T26123	amod	white,arrow
R7288	T26125	T26123	punct	),arrow
R7289	T26126	T26096	punct	.,embryo
R7290	T26128	T26129	punct	(,D
R7291	T26129	T26130	meta	D,staining
R7292	T26131	T26129	punct	),D
R7293	T26132	T26130	compound	Control,staining
R7294	T26133	T26130	compound	background,staining
R7295	T26134	T26135	compound	X,gal
R7296	T26135	T26130	compound	gal,staining
R7297	T26136	T26135	punct	-,gal
R7298	T26137	T26130	prep	of,staining
R7299	T26138	T26139	det	a,tract
R7300	T26139	T26137	pobj	tract,of
R7301	T26140	T26139	amod	urogenital,tract
R7302	T26141	T26139	prep	from,tract
R7303	T26142	T26143	det	an,embryo
R7304	T26143	T26141	pobj	embryo,from
R7305	T26144	T26143	compound	E13.5,embryo
R7306	T26145	T26143	prep	without,embryo
R7307	T26146	T26147	det	the,transgene
R7308	T26147	T26145	pobj	transgene,without
R7309	T26148	T26149	compound	BAT,gal
R7310	T26149	T26147	compound	gal,transgene
R7311	T26150	T26149	punct	-,gal
R7312	T26151	T26139	punct	(,tract
R7313	T26152	T26139	appos	Non-Tg,tract
R7314	T26153	T26139	punct	),tract
R7315	T26154	T26139	punct	", ",tract
R7316	T26155	T26139	acl	showing,tract
R7317	T26156	T26155	dobj	absence,showing
R7318	T26157	T26156	prep	of,absence
R7319	T26158	T26159	amod	endogenous,activity
R7320	T26159	T26157	pobj	activity,of
R7321	T26160	T26161	compound	beta,galactosidase
R7322	T26161	T26159	compound	galactosidase,activity
R7323	T26162	T26161	punct	-,galactosidase
R7324	T26163	T26130	punct	.,staining
R7325	T26165	T26166	punct	(,E
R7326	T26166	T26167	meta	E,Pygo1
R7327	T26168	T26166	punct	),E
R7328	T26169	T26167	punct	+,Pygo1
R7329	T26170	T26167	punct	/,Pygo1
R7330	T26171	T26167	punct	-,Pygo1
R7331	T26172	T26167	punct	/,Pygo1
R7332	T26173	T26167	appos	Pygo2,Pygo1
R7333	T26174	T26173	punct	+,Pygo2
R7334	T26175	T26173	punct	/,Pygo2
R7335	T26176	T26173	punct	+,Pygo2
R7336	T26177	T26167	punct	", ",Pygo1
R7337	T26178	T26167	prep	with,Pygo1
R7338	T26179	T26180	compound	BAT,gal
R7339	T26180	T26182	compound	gal,reporter
R7340	T26181	T26180	punct	-,gal
R7341	T26182	T26183	compound	reporter,activity
R7342	T26183	T26178	pobj	activity,with
R7343	T26184	T26167	prep	in,Pygo1
R7344	T26185	T26186	det	the,compartment
R7345	T26186	T26184	pobj	compartment,in
R7346	T26187	T26186	amod	ureteric,compartment
R7347	T26188	T26186	prep	of,compartment
R7348	T26189	T26190	det	the,kidney
R7349	T26190	T26188	pobj	kidney,of
R7350	T26191	T26190	amod	developing,kidney
R7351	T26192	T26186	punct	", ",compartment
R7352	T26193	T26186	prep	including,compartment
R7353	T26194	T26195	det	the,tips
R7354	T26195	T26193	pobj	tips,including
R7355	T26196	T26195	amod	ureteric,tips
R7356	T26197	T26195	punct	", ",tips
R7357	T26198	T26199	amod	ureteric,tree
R7358	T26199	T26195	conj	tree,tips
R7359	T26200	T26199	punct	", ",tree
R7360	T26201	T26199	cc	and,tree
R7361	T26202	T26199	conj	ureter,tree
R7362	T26203	T26167	punct	.,Pygo1
R7363	T26205	T26206	punct	(,F
R7364	T26206	T26207	meta	F,Pygo1
R7365	T26208	T26206	punct	),F
R7366	T26209	T26207	punct	+,Pygo1
R7367	T26210	T26207	punct	/,Pygo1
R7368	T26211	T26207	punct	-,Pygo1
R7369	T26212	T26207	punct	/,Pygo1
R7370	T26213	T26207	appos	Pygo2,Pygo1
R7371	T26214	T26213	punct	-,Pygo2
R7372	T26215	T26213	punct	/,Pygo2
R7373	T26216	T26213	punct	-,Pygo2
R7374	T26217	T26207	punct	", ",Pygo1
R7375	T26218	T26207	prep	with,Pygo1
R7376	T26219	T26220	amod	marked,reduction
R7377	T26220	T26218	pobj	reduction,with
R7378	T26221	T26220	prep	of,reduction
R7379	T26222	T26223	compound	BAT,gal
R7380	T26223	T26225	compound	gal,reporter
R7381	T26224	T26223	punct	-,gal
R7382	T26225	T26226	compound	reporter,activity
R7383	T26226	T26221	pobj	activity,of
R7384	T26227	T26220	prep	in,reduction
R7385	T26228	T26229	det	the,compartment
R7386	T26229	T26227	pobj	compartment,in
R7387	T26230	T26229	amod	ureteric,compartment
R7388	T26231	T26207	punct	.,Pygo1
R7389	T26233	T26234	punct	(,G
R7390	T26234	T26235	meta	G,Pygo1
R7391	T26236	T26234	punct	),G
R7392	T26237	T26235	punct	+,Pygo1
R7393	T26238	T26235	punct	/,Pygo1
R7394	T26239	T26235	punct	-,Pygo1
R7395	T26240	T26235	punct	/,Pygo1
R7396	T26241	T26235	appos	Pygo2,Pygo1
R7397	T26242	T26241	punct	+,Pygo2
R7398	T26243	T26241	punct	/,Pygo2
R7399	T26244	T26241	punct	-,Pygo2
R7400	T26245	T26235	punct	", ",Pygo1
R7401	T26246	T26235	prep	with,Pygo1
R7402	T26247	T26248	compound	reporter,expression
R7403	T26248	T26246	pobj	expression,with
R7404	T26249	T26248	prep	in,expression
R7405	T26250	T26251	det	the,duct
R7406	T26251	T26249	pobj	duct,in
R7407	T26252	T26251	amod	paramesonephric,duct
R7408	T26253	T26254	punct	(,arrowhead
R7409	T26254	T26251	parataxis	arrowhead,duct
R7410	T26255	T26254	amod	white,arrowhead
R7411	T26256	T26254	punct	),arrowhead
R7412	T26257	T26251	cc	and,duct
R7413	T26258	T26259	amod	ureteric,tree
R7414	T26259	T26251	conj	tree,duct
R7415	T26260	T26235	punct	.,Pygo1
R7416	T26262	T26263	punct	(,H
R7417	T26263	T26264	meta	H,Pygo1
R7418	T26265	T26263	punct	", ",H
R7419	T26266	T26263	dep	I,H
R7420	T26267	T26263	punct	),H
R7421	T26268	T26264	punct	-,Pygo1
R7422	T26269	T26264	punct	/,Pygo1
R7423	T26270	T26264	punct	-,Pygo1
R7424	T26271	T26264	punct	/,Pygo1
R7425	T26272	T26264	appos	Pygo2,Pygo1
R7426	T26273	T26272	punct	+,Pygo2
R7427	T26274	T26272	punct	/,Pygo2
R7428	T26275	T26272	punct	-,Pygo2
R7429	T26276	T26264	punct	", ",Pygo1
R7430	T26277	T26264	prep	with,Pygo1
R7431	T26278	T26279	punct	(,H
R7432	T26279	T26280	meta	H,view
R7433	T26280	T26277	pobj	view,with
R7434	T26281	T26279	punct	),H
R7435	T26282	T26280	amod	ventral,view
R7436	T26283	T26280	acl	showing,view
R7437	T26284	T26285	compound	reporter,expression
R7438	T26285	T26283	dobj	expression,showing
R7439	T26286	T26283	prep	in,showing
R7440	T26287	T26288	det	the,duct
R7441	T26288	T26286	pobj	duct,in
R7442	T26289	T26288	amod	paramesonephric,duct
R7443	T26290	T26291	punct	(,arrowhead
R7444	T26291	T26283	parataxis	arrowhead,showing
R7445	T26292	T26291	amod	white,arrowhead
R7446	T26293	T26291	punct	),arrowhead
R7447	T26294	T26280	punct	", ",view
R7448	T26295	T26280	cc	and,view
R7449	T26296	T26297	punct	(,I
R7450	T26297	T26298	meta	I,view
R7451	T26298	T26280	conj	view,view
R7452	T26299	T26297	punct	),I
R7453	T26300	T26298	amod	dorsal,view
R7454	T26301	T26298	acl	showing,view
R7455	T26302	T26303	amod	ureteric,tree
R7456	T26303	T26304	compound	tree,expression
R7457	T26304	T26301	dobj	expression,showing
R7458	T26305	T26301	prep	in,showing
R7459	T26306	T26307	det	the,kidney
R7460	T26307	T26305	pobj	kidney,in
R7461	T26308	T26264	punct	.,Pygo1
R7462	T26310	T26311	punct	(,J
R7463	T26311	T26312	meta	J,Pygo1
R7464	T26313	T26311	punct	),J
R7465	T26314	T26312	punct	+,Pygo1
R7466	T26315	T26312	punct	/,Pygo1
R7467	T26316	T26312	punct	-,Pygo1
R7468	T26317	T26312	punct	/,Pygo1
R7469	T26318	T26312	appos	Pygo2,Pygo1
R7470	T26319	T26318	punct	+,Pygo2
R7471	T26320	T26318	punct	/,Pygo2
R7472	T26321	T26318	punct	-,Pygo2
R7473	T26322	T26312	punct	", ",Pygo1
R7474	T26323	T26324	det	a,control
R7475	T26324	T26312	appos	control,Pygo1
R7476	T26325	T26324	acl	processed,control
R7477	T26326	T26325	prep	in,processed
R7478	T26327	T26326	pobj	parallel,in
R7479	T26328	T26325	prep	with,processed
R7480	T26329	T26328	punct	(,with
R7481	T26330	T26328	pobj	K,with
R7482	T26331	T26330	punct	),K
R7483	T26332	T26330	cc	and,K
R7484	T26333	T26334	punct	(,L
R7485	T26334	T26330	conj	L,K
R7486	T26335	T26312	punct	),Pygo1
R7487	T26336	T26312	punct	", ",Pygo1
R7488	T26337	T26312	prep	with,Pygo1
R7489	T26338	T26337	pobj	expression,with
R7490	T26339	T26338	prep	in,expression
R7491	T26340	T26341	amod	ureteric,tree
R7492	T26341	T26339	pobj	tree,in
R7493	T26342	T26341	cc	and,tree
R7494	T26343	T26344	amod	paramesonephric,duct
R7495	T26344	T26341	conj	duct,tree
R7496	T26345	T26312	punct	.,Pygo1
R7497	T26347	T26348	punct	(,K
R7498	T26348	T26349	meta	K,lost
R7499	T26350	T26348	punct	", ",K
R7500	T26351	T26348	dep	L,K
R7501	T26352	T26348	punct	),K
R7502	T26353	T26354	nmod	Pygo1,reporter
R7503	T26354	T26364	compound	reporter,activity
R7504	T26355	T26353	punct	-,Pygo1
R7505	T26356	T26353	punct	/,Pygo1
R7506	T26357	T26353	punct	-,Pygo1
R7507	T26358	T26353	punct	/,Pygo1
R7508	T26359	T26353	appos	Pygo2,Pygo1
R7509	T26360	T26359	punct	-,Pygo2
R7510	T26361	T26359	punct	/,Pygo2
R7511	T26362	T26359	punct	-,Pygo2
R7512	T26363	T26354	punct	", ",reporter
R7513	T26364	T26349	nsubjpass	activity,lost
R7514	T26365	T26349	auxpass	was,lost
R7515	T26366	T26349	prep	in,lost
R7516	T26367	T26368	det	the,duct
R7517	T26368	T26366	pobj	duct,in
R7518	T26369	T26368	amod	paramesonephric,duct
R7519	T26370	T26371	punct	(,arrowhead
R7520	T26371	T26368	parataxis	arrowhead,duct
R7521	T26372	T26371	amod	white,arrowhead
R7522	T26373	T26371	punct	),arrowhead
R7523	T26374	T26366	punct	", ",in
R7524	T26375	T26366	cc	and,in
R7525	T26376	T26366	conj	in,in
R7526	T26377	T26378	det	the,ureter
R7527	T26378	T26376	pobj	ureter,in
R7528	T26379	T26378	cc	and,ureter
R7529	T26380	T26381	amod	ureteric,compartment
R7530	T26381	T26378	conj	compartment,ureter
R7531	T26382	T26381	prep	of,compartment
R7532	T26383	T26384	det	the,kidneys
R7533	T26384	T26382	pobj	kidneys,of
R7534	T26385	T26384	amod	developing,kidneys
R7535	T26386	T26387	punct	(,circles
R7536	T26387	T26378	parataxis	circles,ureter
R7537	T26388	T26387	amod	dashed,circles
R7538	T26389	T26387	punct	),circles
R7539	T26390	T26349	punct	", ",lost
R7540	T26391	T26349	prep	except,lost
R7541	T26392	T26391	prep	for,except
R7542	T26393	T26394	punct	(,K
R7543	T26394	T26395	parataxis	K,cells
R7544	T26395	T26392	pobj	cells,for
R7545	T26396	T26394	punct	),K
R7546	T26397	T26395	det	a,cells
R7547	T26398	T26395	amod	few,cells
R7548	T26399	T26400	advmod	faintly,staining
R7549	T26400	T26395	amod	staining,cells
R7550	T26401	T26349	punct	.,lost
R7551	T26403	T26404	punct	(,M
R7552	T26404	T26405	meta	M,kidneys
R7553	T26406	T26404	punct	", ",M
R7554	T26407	T26404	dep	N,M
R7555	T26408	T26404	punct	),M
R7556	T26409	T26405	nmod	Pygo1,kidneys
R7557	T26410	T26409	punct	+,Pygo1
R7558	T26411	T26409	punct	/,Pygo1
R7559	T26412	T26409	punct	-,Pygo1
R7560	T26413	T26409	punct	/,Pygo1
R7561	T26414	T26409	appos	Pygo2,Pygo1
R7562	T26415	T26414	punct	+,Pygo2
R7563	T26416	T26414	punct	/,Pygo2
R7564	T26417	T26414	punct	-,Pygo2
R7565	T26418	T26419	punct	(,left
R7566	T26419	T26409	parataxis	left,Pygo1
R7567	T26420	T26419	punct	),left
R7568	T26421	T26409	cc	and,Pygo1
R7569	T26422	T26409	conj	Pygo1,Pygo1
R7570	T26423	T26422	punct	-,Pygo1
R7571	T26424	T26422	punct	/,Pygo1
R7572	T26425	T26422	punct	-,Pygo1
R7573	T26426	T26422	punct	/,Pygo1
R7574	T26427	T26422	appos	Pygo2,Pygo1
R7575	T26428	T26427	punct	-,Pygo2
R7576	T26429	T26427	punct	/,Pygo2
R7577	T26430	T26427	punct	-,Pygo2
R7578	T26431	T26432	punct	(,right
R7579	T26432	T26422	parataxis	right,Pygo1
R7580	T26433	T26432	punct	),right
R7581	T26434	T26405	compound	E18.5,kidneys
R7582	T26435	T26405	punct	.,kidneys
R7583	T26437	T26438	det	The,kidneys
R7584	T26438	T26439	nsubjpass	kidneys,bisected
R7585	T26440	T26438	prep	in,kidneys
R7586	T26441	T26440	punct	(,in
R7587	T26442	T26440	pobj	N,in
R7588	T26443	T26439	punct	),bisected
R7589	T26444	T26439	auxpass	were,bisected
R7590	T26445	T26439	punct	.,bisected
R7591	T26447	T26448	compound	BAT,gal
R7592	T26448	T26450	compound	gal,reporter
R7593	T26449	T26448	punct	-,gal
R7594	T26450	T26451	compound	reporter,expression
R7595	T26451	T26452	nsubjpass	expression,seen
R7596	T26453	T26452	auxpass	was,seen
R7597	T26454	T26452	prep	in,seen
R7598	T26455	T26456	det	the,components
R7599	T26456	T26454	pobj	components,in
R7600	T26457	T26458	amod	ureteric,tree
R7601	T26458	T26456	compound	tree,components
R7602	T26459	T26456	prep	of,components
R7603	T26460	T26461	det	the,cortex
R7604	T26461	T26459	pobj	cortex,of
R7605	T26462	T26461	cc	and,cortex
R7606	T26463	T26461	conj	medulla,cortex
R7607	T26464	T26461	prep	of,cortex
R7608	T26465	T26466	det	the,heterozygotes
R7609	T26466	T26464	pobj	heterozygotes,of
R7610	T26467	T26466	amod	double,heterozygotes
R7611	T26468	T26452	cc	but,seen
R7612	T26469	T26470	auxpass	was,lost
R7613	T26470	T26452	conj	lost,seen
R7614	T26471	T26472	advmod	almost,completely
R7615	T26472	T26470	advmod	completely,lost
R7616	T26473	T26470	prep	in,lost
R7617	T26474	T26475	det	the,mutants
R7618	T26475	T26473	pobj	mutants,in
R7619	T26476	T26477	amod	double,homozygous
R7620	T26477	T26475	amod	homozygous,mutants
R7621	T26478	T26477	punct	-,homozygous
R7622	T26479	T26452	punct	.,seen
R7623	T26481	T26482	punct	(,O
R7624	T26482	T26483	meta	O,kidneys
R7625	T26484	T26482	punct	),O
R7626	T26485	T26483	nmod	Pygo1,kidneys
R7627	T26486	T26485	punct	+,Pygo1
R7628	T26487	T26485	punct	/,Pygo1
R7629	T26488	T26485	punct	-,Pygo1
R7630	T26489	T26485	punct	/,Pygo1
R7631	T26490	T26485	appos	Pygo2,Pygo1
R7632	T26491	T26490	punct	+,Pygo2
R7633	T26492	T26490	punct	/,Pygo2
R7634	T26493	T26490	punct	+,Pygo2
R7635	T26494	T26495	punct	(,left
R7636	T26495	T26485	parataxis	left,Pygo1
R7637	T26496	T26495	punct	),left
R7638	T26497	T26485	punct	", ",Pygo1
R7639	T26498	T26485	conj	Pygo1,Pygo1
R7640	T26499	T26498	punct	-,Pygo1
R7641	T26500	T26498	punct	/,Pygo1
R7642	T26501	T26498	punct	-,Pygo1
R7643	T26502	T26498	punct	/,Pygo1
R7644	T26503	T26498	appos	Pygo2,Pygo1
R7645	T26504	T26503	punct	+,Pygo2
R7646	T26505	T26503	punct	/,Pygo2
R7647	T26506	T26503	punct	-,Pygo2
R7648	T26507	T26508	punct	(,middle
R7649	T26508	T26498	parataxis	middle,Pygo1
R7650	T26509	T26508	punct	),middle
R7651	T26510	T26498	punct	", ",Pygo1
R7652	T26511	T26498	cc	and,Pygo1
R7653	T26512	T26498	conj	Pygo1,Pygo1
R7654	T26513	T26512	punct	-,Pygo1
R7655	T26514	T26512	punct	/,Pygo1
R7656	T26515	T26512	punct	-,Pygo1
R7657	T26516	T26512	punct	/,Pygo1
R7658	T26517	T26512	appos	Pygo2,Pygo1
R7659	T26518	T26517	punct	-,Pygo2
R7660	T26519	T26517	punct	/,Pygo2
R7661	T26520	T26517	punct	-,Pygo2
R7662	T26521	T26522	punct	(,right
R7663	T26522	T26512	parataxis	right,Pygo1
R7664	T26523	T26522	punct	),right
R7665	T26524	T26483	compound	E18.5,kidneys
R7666	T26525	T26483	punct	.,kidneys
R7667	T26527	T26528	compound	Reporter,activity
R7668	T26528	T26529	nsubj	activity,was
R7669	T26530	T26529	acomp	present,was
R7670	T26531	T26529	prep	in,was
R7671	T26532	T26533	det	the,compartments
R7672	T26533	T26531	pobj	compartments,in
R7673	T26534	T26533	amod	ureteric,compartments
R7674	T26535	T26533	prep	of,compartments
R7675	T26536	T26537	det	the,kidneys
R7676	T26537	T26535	pobj	kidneys,of
R7677	T26538	T26537	nmod	Pygo1,kidneys
R7678	T26539	T26538	punct	+,Pygo1
R7679	T26540	T26538	punct	/,Pygo1
R7680	T26541	T26538	punct	-,Pygo1
R7681	T26542	T26538	punct	/,Pygo1
R7682	T26543	T26538	appos	Pygo2,Pygo1
R7683	T26544	T26543	punct	+,Pygo2
R7684	T26545	T26543	punct	/,Pygo2
R7685	T26546	T26543	punct	+,Pygo2
R7686	T26547	T26548	punct	(,left
R7687	T26548	T26538	parataxis	left,Pygo1
R7688	T26549	T26548	punct	),left
R7689	T26550	T26538	cc	and,Pygo1
R7690	T26551	T26538	conj	Pygo1,Pygo1
R7691	T26552	T26551	punct	-,Pygo1
R7692	T26553	T26551	punct	/,Pygo1
R7693	T26554	T26551	punct	-,Pygo1
R7694	T26555	T26551	punct	/,Pygo1
R7695	T26556	T26551	appos	Pygo2,Pygo1
R7696	T26557	T26556	punct	+,Pygo2
R7697	T26558	T26556	punct	/,Pygo2
R7698	T26559	T26556	punct	-,Pygo2
R7699	T26560	T26561	punct	(,middle
R7700	T26561	T26551	parataxis	middle,Pygo1
R7701	T26562	T26561	punct	),middle
R7702	T26563	T26529	punct	", ",was
R7703	T26564	T26529	cc	but,was
R7704	T26565	T26529	conj	lost,was
R7705	T26566	T26565	prep	in,lost
R7706	T26567	T26568	det	the,kidney
R7707	T26568	T26566	pobj	kidney,in
R7708	T26569	T26568	nmod	Pygo1,kidney
R7709	T26570	T26569	punct	-,Pygo1
R7710	T26571	T26569	punct	/,Pygo1
R7711	T26572	T26569	punct	-,Pygo1
R7712	T26573	T26569	punct	/,Pygo1
R7713	T26574	T26569	appos	Pygo2,Pygo1
R7714	T26575	T26574	punct	-,Pygo2
R7715	T26576	T26574	punct	/,Pygo2
R7716	T26577	T26574	punct	-,Pygo2
R7717	T26578	T26579	punct	(,right
R7718	T26579	T26569	parataxis	right,Pygo1
R7719	T26580	T26579	punct	),right
R7720	T26581	T26529	punct	.,was
R7721	T26583	T26584	amod	Original,magnification
R7722	T26584	T26585	dep	magnification,32
R7723	T26586	T26585	punct	: ,32
R7724	T26587	T26588	punct	(,A
R7725	T26588	T26585	parataxis	A,32
R7726	T26589	T26590	punct	-,C
R7727	T26590	T26588	prep	C,A
R7728	T26591	T26588	punct	),A
R7729	T26592	T26585	punct	×,32
R7730	T26593	T26585	punct	", ",32
R7731	T26594	T26595	punct	(,D
R7732	T26595	T26596	parataxis	D,63
R7733	T26596	T26585	conj	63,32
R7734	T26597	T26598	punct	-,F
R7735	T26598	T26595	prep	F,D
R7736	T26599	T26595	punct	),D
R7737	T26600	T26596	punct	×,63
R7738	T26601	T26596	punct	", ",63
R7739	T26602	T26603	punct	(,G
R7740	T26603	T26604	parataxis	G,40
R7741	T26604	T26596	conj	40,63
R7742	T26605	T26606	punct	-,I
R7743	T26606	T26603	prep	I,G
R7744	T26607	T26603	punct	),G
R7745	T26608	T26604	punct	×,40
R7746	T26609	T26604	punct	", ",40
R7747	T26610	T26611	punct	(,J
R7748	T26611	T26612	parataxis	J,50
R7749	T26612	T26604	conj	50,40
R7750	T26613	T26614	punct	-,L
R7751	T26614	T26611	prep	L,J
R7752	T26615	T26611	punct	),J
R7753	T26616	T26612	punct	×,50
R7754	T26617	T26612	punct	", ",50
R7755	T26618	T26619	punct	(,N
R7756	T26619	T26622	parataxis	N,10
R7757	T26620	T26619	nmod	M,N
R7758	T26621	T26619	punct	", ",N
R7759	T26622	T26612	conj	10,50
R7760	T26623	T26619	punct	),N
R7761	T26624	T26622	punct	×,10
R7762	T26625	T26622	punct	", ",10
R7763	T26626	T26622	cc	and,10
R7764	T26627	T26628	punct	(,O
R7765	T26628	T26629	parataxis	O,12.5
R7766	T26629	T26622	conj	12.5,10
R7767	T26630	T26628	punct	),O
R7768	T26631	T26629	punct	×,12.5
R7769	T26632	T26585	punct	.,32
R7770	T26917	T26918	amod	Quantitative,analysis
R7771	T26919	T26918	prep	of,analysis
R7772	T26920	T26921	compound	BAT,gal
R7773	T26921	T26923	compound	gal,reporter
R7774	T26922	T26921	punct	-,gal
R7775	T26923	T26924	compound	reporter,expression
R7776	T26924	T26919	pobj	expression,of
R7777	T26925	T26924	prep	in,expression
R7778	T26926	T26927	compound	Pygo1,Pygo2
R7779	T26927	T26929	compound	Pygo2,extracts
R7780	T26928	T26927	punct	/,Pygo2
R7781	T26929	T26925	pobj	extracts,in
R7782	T26930	T26929	compound	E18.5,extracts
R7783	T26931	T26929	compound	kidney,extracts
R7784	T26932	T26918	punct	.,analysis
R7785	T26934	T26935	npadvmod	Transgene,specific
R7786	T26935	T26937	amod	specific,galactosidase
R7787	T26936	T26935	punct	-,specific
R7788	T26937	T26940	nsubjpass	galactosidase,quantified
R7789	T26938	T26937	compound	beta,galactosidase
R7790	T26939	T26937	punct	-,galactosidase
R7791	T26941	T26940	auxpass	was,quantified
R7792	T26942	T26940	prep	by,quantified
R7793	T26943	T26944	compound	ELISA,analysis
R7794	T26944	T26942	pobj	analysis,by
R7795	T26945	T26940	punct	.,quantified
R7796	T26947	T26948	nmod	Pygo1,kidneys
R7797	T26948	T26977	nsubj	kidneys,showed
R7798	T26949	T26947	punct	+,Pygo1
R7799	T26950	T26947	punct	/,Pygo1
R7800	T26951	T26947	punct	-,Pygo1
R7801	T26952	T26947	punct	/,Pygo1
R7802	T26953	T26947	appos	Pygo2,Pygo1
R7803	T26954	T26953	punct	+,Pygo2
R7804	T26955	T26953	punct	/,Pygo2
R7805	T26956	T26953	punct	+,Pygo2
R7806	T26957	T26947	punct	", ",Pygo1
R7807	T26958	T26947	conj	Pygo1,Pygo1
R7808	T26959	T26958	punct	+,Pygo1
R7809	T26960	T26958	punct	/,Pygo1
R7810	T26961	T26958	punct	-,Pygo1
R7811	T26962	T26958	punct	/,Pygo1
R7812	T26963	T26958	appos	Pygo2,Pygo1
R7813	T26964	T26963	punct	+,Pygo2
R7814	T26965	T26963	punct	/,Pygo2
R7815	T26966	T26963	punct	-,Pygo2
R7816	T26967	T26958	cc	and,Pygo1
R7817	T26968	T26958	conj	Pygo1,Pygo1
R7818	T26969	T26968	punct	+,Pygo1
R7819	T26970	T26968	punct	/,Pygo1
R7820	T26971	T26968	punct	-,Pygo1
R7821	T26972	T26968	punct	/,Pygo1
R7822	T26973	T26968	appos	Pygo2,Pygo1
R7823	T26974	T26973	punct	+,Pygo2
R7824	T26975	T26973	punct	/,Pygo2
R7825	T26976	T26973	punct	+,Pygo2
R7826	T26978	T26948	appos	all,kidneys
R7827	T26979	T26980	amod	similar,levels
R7828	T26980	T26977	dobj	levels,showed
R7829	T26981	T26980	prep	of,levels
R7830	T26982	T26983	compound	BAT,gal
R7831	T26983	T26985	compound	gal,expression
R7832	T26984	T26983	punct	-,gal
R7833	T26985	T26981	pobj	expression,of
R7834	T26986	T26977	punct	.,showed
R7835	T26988	T26989	prep	In,reduced
R7836	T26990	T26991	det	the,mutants
R7837	T26991	T26988	pobj	mutants,In
R7838	T26992	T26993	nmod	Pygo2,hetereozygote
R7839	T26993	T26991	nmod	hetereozygote,mutants
R7840	T26994	T26991	punct	", ",mutants
R7841	T26995	T26996	npadvmod	Pygo1,homozygous
R7842	T26996	T26991	amod	homozygous,mutants
R7843	T26997	T26989	punct	", ",reduced
R7844	T26998	T26989	advmod	however,reduced
R7845	T26999	T26989	punct	", ",reduced
R7846	T27000	T27001	compound	reporter,expression
R7847	T27001	T26989	nsubjpass	expression,reduced
R7848	T27002	T26989	auxpass	was,reduced
R7849	T27003	T26989	prep	by,reduced
R7850	T27004	T27005	quantmod	about,50
R7851	T27005	T27006	nummod	50,%
R7852	T27006	T27003	pobj	%,by
R7853	T27007	T26989	punct	.,reduced
R7854	T27009	T27010	prep	In,was
R7855	T27011	T27012	npadvmod	Pygo2,homozygous
R7856	T27012	T27013	amod	homozygous,mutants
R7857	T27013	T27009	pobj	mutants,In
R7858	T27014	T27015	compound	BAT,gal
R7859	T27015	T27017	compound	gal,expression
R7860	T27016	T27015	punct	-,gal
R7861	T27017	T27010	nsubj	expression,was
R7862	T27018	T27019	advmod	uniformly,low
R7863	T27019	T27010	acomp	low,was
R7864	T27020	T27010	punct	", ",was
R7865	T27021	T27010	prep	with,was
R7866	T27022	T27021	cc	or,with
R7867	T27023	T27021	conj	without,with
R7868	T27024	T27025	amod	wild,type
R7869	T27025	T27027	compound	type,alleles
R7870	T27026	T27025	punct	-,type
R7871	T27027	T27023	pobj	alleles,without
R7872	T27028	T27027	compound	Pygo1,alleles
R7873	T27029	T27010	punct	.,was
R7874	T27031	T27032	det	Each,group
R7875	T27032	T27034	nsubj	group,included
R7876	T27033	T27032	amod	experimental,group
R7877	T27035	T27036	det	a,size
R7878	T27036	T27034	dobj	size,included
R7879	T27037	T27036	compound	sample,size
R7880	T27038	T27036	prep	of,size
R7881	T27039	T27040	advmod	at,four
R7882	T27040	T27038	pobj	four,of
R7883	T27041	T27040	advmod	least,four
R7884	T27042	T27034	punct	.,included

PMC:1858683 / 13634-23616 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

PMC:1858683 / 13634-23616 JSON TXT 5 Projects