PMC:1858683 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T944	6-9	CC	denotes	and
T945	10-15	NN	denotes	Pygo2
T943	16-21	NNS	denotes	roles
T946	22-24	IN	denotes	in
T947	25-28	NN	denotes	Wnt
T948	29-38	NN	denotes	signaling
T949	39-41	IN	denotes	in
T950	42-51	JJ	denotes	mammalian
T951	52-58	NN	denotes	kidney
T952	59-70	NN	denotes	development
T953	70-71	sentence	denotes
T954	81-91	sentence	denotes	Background
T955	81-91	NN	denotes	Background
T956	91-239	sentence	denotes	The pygopus gene of Drosophila encodes an essential component of the Armadillo (β-catenin) transcription factor complex of canonical Wnt signaling.
T957	92-95	DT	denotes	The
T959	96-103	NN	denotes	pygopus
T958	104-108	NN	denotes	gene
T961	109-111	IN	denotes	of
T962	112-122	NNP	denotes	Drosophila
T960	123-130	VBZ	denotes	encodes
T963	131-133	DT	denotes	an
T965	134-143	JJ	denotes	essential
T964	144-153	NN	denotes	component
T966	154-156	IN	denotes	of
T967	157-160	DT	denotes	the
T969	161-170	NN	denotes	Armadillo
T971	171-172	-LRB-	denotes	(
T973	172-173	NN	denotes	β
T974	173-174	HYPH	denotes	-
T972	174-181	NN	denotes	catenin
T975	181-182	-RRB-	denotes	)
T976	183-196	NN	denotes	transcription
T970	197-203	NN	denotes	factor
T968	204-211	NN	denotes	complex
T977	212-214	IN	denotes	of
T978	215-224	JJ	denotes	canonical
T980	225-228	NN	denotes	Wnt
T979	229-238	NN	denotes	signaling
T981	238-239	.	denotes	.
T982	239-419	sentence	denotes	To better understand the functions of Pygopus-mediated canonical Wnt signaling in kidney development, targeted mutations were made in the two mammalian orthologs, Pygo1 and Pygo2.
T983	240-242	TO	denotes	To
T985	243-249	RBR	denotes	better
T984	250-260	VB	denotes	understand
T987	261-264	DT	denotes	the
T988	265-274	NNS	denotes	functions
T989	275-277	IN	denotes	of
T990	278-285	NN	denotes	Pygopus
T992	285-286	HYPH	denotes	-
T991	286-294	VBN	denotes	mediated
T994	295-304	JJ	denotes	canonical
T995	305-308	NN	denotes	Wnt
T993	309-318	NN	denotes	signaling
T996	319-321	IN	denotes	in
T997	322-328	NN	denotes	kidney
T998	329-340	NN	denotes	development
T999	340-342	,	denotes	,
T1000	342-350	VBN	denotes	targeted
T1001	351-360	NNS	denotes	mutations
T1002	361-365	VBD	denotes	were
T986	366-370	VBN	denotes	made
T1003	371-373	IN	denotes	in
T1004	374-377	DT	denotes	the
T1006	378-381	CD	denotes	two
T1007	382-391	JJ	denotes	mammalian
T1005	392-401	NNS	denotes	orthologs
T1008	401-403	,	denotes	,
T1009	403-408	NN	denotes	Pygo1
T1010	409-412	CC	denotes	and
T1011	413-418	NN	denotes	Pygo2
T1012	418-419	.	denotes	.
T1013	419-428	sentence	denotes	Results
T1014	421-428	NNS	denotes	Results
T1015	428-562	sentence	denotes	Each mutation deleted >80% of the coding sequence, including the critical PHD domain, and almost certainly resulted in null function.
T1016	429-433	DT	denotes	Each
T1017	434-442	NN	denotes	mutation
T1019	443-450	VBD	denotes	deleted
T1020	451-452	SYM	denotes	>
T1021	452-454	CD	denotes	80
T1022	454-455	NN	denotes	%
T1023	456-458	IN	denotes	of
T1024	459-462	DT	denotes	the
T1026	463-469	NN	denotes	coding
T1025	470-478	NN	denotes	sequence
T1027	478-480	,	denotes	,
T1028	480-489	VBG	denotes	including
T1029	490-493	DT	denotes	the
T1031	494-502	JJ	denotes	critical
T1032	503-506	NN	denotes	PHD
T1030	507-513	NN	denotes	domain
T1033	513-515	,	denotes	,
T1034	515-518	CC	denotes	and
T1035	519-525	RB	denotes	almost
T1036	526-535	RB	denotes	certainly
T1018	536-544	VBD	denotes	resulted
T1037	545-547	IN	denotes	in
T1038	548-552	JJ	denotes	null
T1039	553-561	NN	denotes	function
T1040	561-562	.	denotes	.
T1041	562-773	sentence	denotes	Pygo2 homozygous mutants, with rare exception, died shortly after birth, with a phenotype including lens agenesis, growth retardation, altered kidney development, and in some cases exencephaly and cleft palate.
T1042	563-568	NN	denotes	Pygo2
T1043	569-579	JJ	denotes	homozygous
T1044	580-587	NNS	denotes	mutants
T1046	587-589	,	denotes	,
T1047	589-593	IN	denotes	with
T1048	594-598	JJ	denotes	rare
T1049	599-608	NN	denotes	exception
T1050	608-610	,	denotes	,
T1045	610-614	VBD	denotes	died
T1051	615-622	RB	denotes	shortly
T1052	623-628	IN	denotes	after
T1053	629-634	NN	denotes	birth
T1054	634-636	,	denotes	,
T1055	636-640	IN	denotes	with
T1056	641-642	DT	denotes	a
T1057	643-652	NN	denotes	phenotype
T1058	653-662	VBG	denotes	including
T1059	663-667	NN	denotes	lens
T1060	668-676	NN	denotes	agenesis
T1061	676-678	,	denotes	,
T1062	678-684	NN	denotes	growth
T1063	685-696	NN	denotes	retardation
T1064	696-698	,	denotes	,
T1065	698-705	VBN	denotes	altered
T1067	706-712	NN	denotes	kidney
T1066	713-724	NN	denotes	development
T1068	724-726	,	denotes	,
T1069	726-729	CC	denotes	and
T1070	730-732	IN	denotes	in
T1072	733-737	DT	denotes	some
T1073	738-743	NNS	denotes	cases
T1071	744-755	NN	denotes	exencephaly
T1074	756-759	CC	denotes	and
T1075	760-765	VBN	denotes	cleft
T1076	766-772	NN	denotes	palate
T1077	772-773	.	denotes	.
T1078	773-875	sentence	denotes	Pygo1 homozygous mutants, however, were viable and fertile, with no detectable developmental defects.
T1079	774-779	NN	denotes	Pygo1
T1080	780-790	JJ	denotes	homozygous
T1081	791-798	NNS	denotes	mutants
T1083	798-800	,	denotes	,
T1084	800-807	RB	denotes	however
T1085	807-809	,	denotes	,
T1082	809-813	VBD	denotes	were
T1086	814-820	JJ	denotes	viable
T1087	821-824	CC	denotes	and
T1088	825-832	JJ	denotes	fertile
T1089	832-834	,	denotes	,
T1090	834-838	IN	denotes	with
T1091	839-841	DT	denotes	no
T1093	842-852	JJ	denotes	detectable
T1094	853-866	JJ	denotes	developmental
T1092	867-874	NNS	denotes	defects
T1095	874-875	.	denotes	.
T1096	875-963	sentence	denotes	Double Pygo1/Pygo2 homozygous mutants showed no apparent synergy in phenotype severity.
T1097	876-882	JJ	denotes	Double
T1099	883-888	NN	denotes	Pygo1
T1100	888-889	HYPH	denotes	/
T1098	889-894	NN	denotes	Pygo2
T1101	895-905	JJ	denotes	homozygous
T1102	906-913	NNS	denotes	mutants
T1103	914-920	VBD	denotes	showed
T1104	921-923	DT	denotes	no
T1106	924-932	JJ	denotes	apparent
T1105	933-940	NN	denotes	synergy
T1107	941-943	IN	denotes	in
T1108	944-953	NN	denotes	phenotype
T1109	954-962	NN	denotes	severity
T1110	962-963	.	denotes	.
T1111	963-1143	sentence	denotes	The BAT-gal transgene reporter of canonical Wnt signaling showed reduced levels of expression in Pygo1-/-/Pygo2-/- mutants, with tissue-specific variation in degree of diminution.
T1112	964-967	DT	denotes	The
T1114	968-971	NN	denotes	BAT
T1116	971-972	HYPH	denotes	-
T1115	972-975	NN	denotes	gal
T1117	976-985	NN	denotes	transgene
T1113	986-994	NN	denotes	reporter
T1119	995-997	IN	denotes	of
T1120	998-1007	JJ	denotes	canonical
T1122	1008-1011	NN	denotes	Wnt
T1121	1012-1021	NN	denotes	signaling
T1118	1022-1028	VBD	denotes	showed
T1123	1029-1036	VBN	denotes	reduced
T1124	1037-1043	NNS	denotes	levels
T1125	1044-1046	IN	denotes	of
T1126	1047-1057	NN	denotes	expression
T1127	1058-1060	IN	denotes	in
T1128	1061-1066	NN	denotes	Pygo1
T1130	1066-1067	SYM	denotes	-
T1131	1067-1068	HYPH	denotes	/
T1132	1068-1069	SYM	denotes	-
T1133	1069-1070	HYPH	denotes	/
T1134	1070-1075	NN	denotes	Pygo2
T1135	1075-1076	SYM	denotes	-
T1136	1076-1077	HYPH	denotes	/
T1137	1077-1078	SYM	denotes	-
T1129	1079-1086	NNS	denotes	mutants
T1138	1086-1088	,	denotes	,
T1139	1088-1092	IN	denotes	with
T1140	1093-1099	NN	denotes	tissue
T1142	1099-1100	HYPH	denotes	-
T1141	1100-1108	JJ	denotes	specific
T1143	1109-1118	NN	denotes	variation
T1144	1119-1121	IN	denotes	in
T1145	1122-1128	NN	denotes	degree
T1146	1129-1131	IN	denotes	of
T1147	1132-1142	NN	denotes	diminution
T1148	1142-1143	.	denotes	.
T1149	1143-1281	sentence	denotes	The Pygo1 and Pygo2 genes both showed widespread expression in the developing kidney, with raised levels in the stromal cell compartment.
T1150	1144-1147	DT	denotes	The
T1152	1148-1153	NN	denotes	Pygo1
T1153	1154-1157	CC	denotes	and
T1154	1158-1163	NN	denotes	Pygo2
T1151	1164-1169	NNS	denotes	genes
T1156	1170-1174	RB	denotes	both
T1155	1175-1181	VBD	denotes	showed
T1157	1182-1192	JJ	denotes	widespread
T1158	1193-1203	NN	denotes	expression
T1159	1204-1206	IN	denotes	in
T1160	1207-1210	DT	denotes	the
T1162	1211-1221	VBG	denotes	developing
T1161	1222-1228	NN	denotes	kidney
T1163	1228-1230	,	denotes	,
T1164	1230-1234	IN	denotes	with
T1165	1235-1241	VBN	denotes	raised
T1166	1242-1248	NNS	denotes	levels
T1167	1249-1251	IN	denotes	in
T1168	1252-1255	DT	denotes	the
T1170	1256-1263	JJ	denotes	stromal
T1171	1264-1268	NN	denotes	cell
T1169	1269-1280	NN	denotes	compartment
T1172	1280-1281	.	denotes	.
T1173	1281-1421	sentence	denotes	Confocal analysis of the double mutant kidneys showed disturbance of both the ureteric bud and metanephric mesenchyme-derived compartments.
T1174	1282-1290	JJ	denotes	Confocal
T1175	1291-1299	NN	denotes	analysis
T1177	1300-1302	IN	denotes	of
T1178	1303-1306	DT	denotes	the
T1180	1307-1313	JJ	denotes	double
T1181	1314-1320	NN	denotes	mutant
T1179	1321-1328	NNS	denotes	kidneys
T1176	1329-1335	VBD	denotes	showed
T1182	1336-1347	NN	denotes	disturbance
T1183	1348-1350	IN	denotes	of
T1184	1351-1355	CC	denotes	both
T1186	1356-1359	DT	denotes	the
T1187	1360-1368	JJ	denotes	ureteric
T1188	1369-1372	NN	denotes	bud
T1190	1373-1376	CC	denotes	and
T1191	1377-1388	JJ	denotes	metanephric
T1192	1389-1399	NN	denotes	mesenchyme
T1193	1399-1400	HYPH	denotes	-
T1189	1400-1407	VBN	denotes	derived
T1185	1408-1420	NNS	denotes	compartments
T1194	1420-1421	.	denotes	.
T1195	1421-1586	sentence	denotes	Branching morphogenesis of the ureteric bud was altered, with expanded tips and reduced tip density, probably contributing to the smaller size of the mutant kidney.
T1196	1422-1431	VBG	denotes	Branching
T1197	1432-1445	NN	denotes	morphogenesis
T1199	1446-1448	IN	denotes	of
T1200	1449-1452	DT	denotes	the
T1202	1453-1461	JJ	denotes	ureteric
T1201	1462-1465	NN	denotes	bud
T1203	1466-1469	VBD	denotes	was
T1198	1470-1477	VBN	denotes	altered
T1204	1477-1479	,	denotes	,
T1205	1479-1483	IN	denotes	with
T1206	1484-1492	VBN	denotes	expanded
T1207	1493-1497	NNS	denotes	tips
T1208	1498-1501	CC	denotes	and
T1209	1502-1509	VBN	denotes	reduced
T1211	1510-1513	NN	denotes	tip
T1210	1514-1521	NN	denotes	density
T1212	1521-1523	,	denotes	,
T1213	1523-1531	RB	denotes	probably
T1214	1532-1544	VBG	denotes	contributing
T1215	1545-1547	IN	denotes	to
T1216	1548-1551	DT	denotes	the
T1218	1552-1559	JJR	denotes	smaller
T1217	1560-1564	NN	denotes	size
T1219	1565-1567	IN	denotes	of
T1220	1568-1571	DT	denotes	the
T1222	1572-1578	NN	denotes	mutant
T1221	1579-1585	NN	denotes	kidney
T1223	1585-1586	.	denotes	.
T1224	1586-1684	sentence	denotes	In addition, there was an expansion of the zone of condensed mesenchyme capping the ureteric bud.
T1225	1587-1589	IN	denotes	In
T1227	1590-1598	NN	denotes	addition
T1228	1598-1600	,	denotes	,
T1229	1600-1605	EX	denotes	there
T1226	1606-1609	VBD	denotes	was
T1230	1610-1612	DT	denotes	an
T1231	1613-1622	NN	denotes	expansion
T1232	1623-1625	IN	denotes	of
T1233	1626-1629	DT	denotes	the
T1234	1630-1634	NN	denotes	zone
T1235	1635-1637	IN	denotes	of
T1236	1638-1647	VBN	denotes	condensed
T1237	1648-1658	NN	denotes	mesenchyme
T1238	1659-1666	VBG	denotes	capping
T1239	1667-1670	DT	denotes	the
T1241	1671-1679	JJ	denotes	ureteric
T1240	1680-1683	NN	denotes	bud
T1242	1683-1684	.	denotes	.
T1243	1684-1732	sentence	denotes	Nephron formation, however, proceeded normally.
T1244	1685-1692	NN	denotes	Nephron
T1245	1693-1702	NN	denotes	formation
T1247	1702-1704	,	denotes	,
T1248	1704-1711	RB	denotes	however
T1249	1711-1713	,	denotes	,
T1246	1713-1722	VBD	denotes	proceeded
T1250	1723-1731	RB	denotes	normally
T1251	1731-1732	.	denotes	.
T1252	1732-1908	sentence	denotes	Microarray analysis showed changed expression of several genes, including Cxcl13, Slc5a2, Klk5, Ren2 and Timeless, which represent candidate Wnt targets in kidney development.
T1253	1733-1743	NN	denotes	Microarray
T1254	1744-1752	NN	denotes	analysis
T1255	1753-1759	VBD	denotes	showed
T1256	1760-1767	VBN	denotes	changed
T1257	1768-1778	NN	denotes	expression
T1258	1779-1781	IN	denotes	of
T1259	1782-1789	JJ	denotes	several
T1260	1790-1795	NNS	denotes	genes
T1261	1795-1797	,	denotes	,
T1262	1797-1806	VBG	denotes	including
T1263	1807-1813	NN	denotes	Cxcl13
T1264	1813-1815	,	denotes	,
T1265	1815-1821	NN	denotes	Slc5a2
T1266	1821-1823	,	denotes	,
T1267	1823-1827	NN	denotes	Klk5
T1268	1827-1829	,	denotes	,
T1269	1829-1833	NN	denotes	Ren2
T1270	1834-1837	CC	denotes	and
T1271	1838-1846	NN	denotes	Timeless
T1272	1846-1848	,	denotes	,
T1273	1848-1853	WDT	denotes	which
T1274	1854-1863	VBP	denotes	represent
T1275	1864-1873	NN	denotes	candidate
T1277	1874-1877	NN	denotes	Wnt
T1276	1878-1885	NNS	denotes	targets
T1278	1886-1888	IN	denotes	in
T1279	1889-1895	NN	denotes	kidney
T1280	1896-1907	NN	denotes	development
T1281	1907-1908	.	denotes	.
T1282	1908-1920	sentence	denotes	Conclusion
T1283	1910-1920	NN	denotes	Conclusion
T1284	1920-2043	sentence	denotes	The mammalian Pygopus genes are required for normal branching morphogenesis of the ureteric bud during kidney development.
T1285	1921-1924	DT	denotes	The
T1287	1925-1934	JJ	denotes	mammalian
T1288	1935-1942	NN	denotes	Pygopus
T1286	1943-1948	NNS	denotes	genes
T1290	1949-1952	VBP	denotes	are
T1289	1953-1961	VBN	denotes	required
T1291	1962-1965	IN	denotes	for
T1292	1966-1972	JJ	denotes	normal
T1294	1973-1982	NN	denotes	branching
T1293	1983-1996	NN	denotes	morphogenesis
T1295	1997-1999	IN	denotes	of
T1296	2000-2003	DT	denotes	the
T1298	2004-2012	JJ	denotes	ureteric
T1297	2013-2016	NN	denotes	bud
T1299	2017-2023	IN	denotes	during
T1300	2024-2030	NN	denotes	kidney
T1301	2031-2042	NN	denotes	development
T1302	2042-2043	.	denotes	.
T1303	2043-2240	sentence	denotes	Nevertheless, the relatively mild phenotype observed in the kidney, as well as other organ systems, indicates a striking evolutionary divergence of Pygopus function between mammals and Drosophila.
T1304	2044-2056	RB	denotes	Nevertheless
T1306	2056-2058	,	denotes	,
T1307	2058-2061	DT	denotes	the
T1309	2062-2072	RB	denotes	relatively
T1310	2073-2077	JJ	denotes	mild
T1308	2078-2087	NN	denotes	phenotype
T1311	2088-2096	VBN	denotes	observed
T1312	2097-2099	IN	denotes	in
T1313	2100-2103	DT	denotes	the
T1314	2104-2110	NN	denotes	kidney
T1315	2110-2112	,	denotes	,
T1316	2112-2114	RB	denotes	as
T1318	2115-2119	RB	denotes	well
T1317	2120-2122	IN	denotes	as
T1319	2123-2128	JJ	denotes	other
T1321	2129-2134	NN	denotes	organ
T1320	2135-2142	NNS	denotes	systems
T1322	2142-2144	,	denotes	,
T1305	2144-2153	VBZ	denotes	indicates
T1323	2154-2155	DT	denotes	a
T1325	2156-2164	JJ	denotes	striking
T1326	2165-2177	JJ	denotes	evolutionary
T1324	2178-2188	NN	denotes	divergence
T1327	2189-2191	IN	denotes	of
T1328	2192-2199	NN	denotes	Pygopus
T1329	2200-2208	NN	denotes	function
T1330	2209-2216	IN	denotes	between
T1331	2217-2224	NNS	denotes	mammals
T1332	2225-2228	CC	denotes	and
T1333	2229-2239	NNP	denotes	Drosophila
T1334	2239-2240	.	denotes	.
T1335	2240-2447	sentence	denotes	In mammals, the Pygo1/Pygo2 genes are not absolutely required for canonical Wnt signaling in most developing systems, but rather function as quantitative transducers, or modulators, of Wnt signal intensity.
T1336	2241-2243	IN	denotes	In
T1338	2244-2251	NNS	denotes	mammals
T1339	2251-2253	,	denotes	,
T1340	2253-2256	DT	denotes	the
T1342	2257-2262	NN	denotes	Pygo1
T1344	2262-2263	HYPH	denotes	/
T1343	2263-2268	NN	denotes	Pygo2
T1341	2269-2274	NNS	denotes	genes
T1345	2275-2278	VBP	denotes	are
T1346	2279-2282	RB	denotes	not
T1347	2283-2293	RB	denotes	absolutely
T1337	2294-2302	VBN	denotes	required
T1348	2303-2306	IN	denotes	for
T1349	2307-2316	JJ	denotes	canonical
T1351	2317-2320	NN	denotes	Wnt
T1350	2321-2330	NN	denotes	signaling
T1352	2331-2333	IN	denotes	in
T1353	2334-2338	JJS	denotes	most
T1355	2339-2349	VBG	denotes	developing
T1354	2350-2357	NNS	denotes	systems
T1356	2357-2359	,	denotes	,
T1357	2359-2362	CC	denotes	but
T1358	2363-2369	RB	denotes	rather
T1359	2370-2378	VBP	denotes	function
T1360	2379-2381	IN	denotes	as
T1361	2382-2394	JJ	denotes	quantitative
T1362	2395-2406	NNS	denotes	transducers
T1363	2406-2408	,	denotes	,
T1364	2408-2410	CC	denotes	or
T1365	2411-2421	NNS	denotes	modulators
T1366	2421-2423	,	denotes	,
T1367	2423-2425	IN	denotes	of
T1368	2426-2429	NN	denotes	Wnt
T1369	2430-2436	NN	denotes	signal
T1370	2437-2446	NN	denotes	intensity
T1371	2446-2447	.	denotes	.
T2638	2460-2463	NN	denotes	Wnt
T2639	2464-2473	NN	denotes	signaling
T2640	2474-2476	VBZ	denotes	is
T2641	2477-2479	IN	denotes	of
T2642	2480-2488	JJ	denotes	critical
T2643	2489-2499	NN	denotes	importance
T2644	2500-2502	IN	denotes	in
T2645	2503-2510	JJ	denotes	several
T2646	2511-2517	NNS	denotes	stages
T2647	2518-2520	IN	denotes	of
T2648	2521-2527	NN	denotes	kidney
T2649	2528-2539	NN	denotes	development
T2650	2539-2540	.	denotes	.
T2651	2540-2647	sentence	denotes	Mutual inductive interactions between the ureteric bud and metanephric mesenchyme drive nephrogenesis [1].
T2652	2541-2547	JJ	denotes	Mutual
T2654	2548-2557	JJ	denotes	inductive
T2653	2558-2570	NNS	denotes	interactions
T2656	2571-2578	IN	denotes	between
T2657	2579-2582	DT	denotes	the
T2659	2583-2591	JJ	denotes	ureteric
T2658	2592-2595	NN	denotes	bud
T2660	2596-2599	CC	denotes	and
T2661	2600-2611	JJ	denotes	metanephric
T2662	2612-2622	NN	denotes	mesenchyme
T2655	2623-2628	VBP	denotes	drive
T2663	2629-2642	NN	denotes	nephrogenesis
T2664	2643-2644	-LRB-	denotes	[
T2665	2644-2645	CD	denotes	1
T2666	2645-2646	-RRB-	denotes	]
T2667	2646-2647	.	denotes	.
T2668	2647-2756	sentence	denotes	The ureteric bud synthesizes Wnt9b, which is essential for induction of the mesenchyme to form nephrons [2].
T2669	2648-2651	DT	denotes	The
T2671	2652-2660	JJ	denotes	ureteric
T2670	2661-2664	NN	denotes	bud
T2672	2665-2676	VBZ	denotes	synthesizes
T2673	2677-2682	NN	denotes	Wnt9b
T2674	2682-2684	,	denotes	,
T2675	2684-2689	WDT	denotes	which
T2676	2690-2692	VBZ	denotes	is
T2677	2693-2702	JJ	denotes	essential
T2678	2703-2706	IN	denotes	for
T2679	2707-2716	NN	denotes	induction
T2680	2717-2719	IN	denotes	of
T2681	2720-2723	DT	denotes	the
T2682	2724-2734	NN	denotes	mesenchyme
T2683	2735-2737	TO	denotes	to
T2684	2738-2742	VB	denotes	form
T2685	2743-2751	NNS	denotes	nephrons
T2686	2752-2753	-LRB-	denotes	[
T2687	2753-2754	CD	denotes	2
T2688	2754-2755	-RRB-	denotes	]
T2689	2755-2756	.	denotes	.
T2690	2756-2851	sentence	denotes	Wnt4 is made by the induced metanephric mesenchyme and is also required for nephrogenesis [3].
T2691	2757-2761	NN	denotes	Wnt4
T2693	2762-2764	VBZ	denotes	is
T2692	2765-2769	VBN	denotes	made
T2694	2770-2772	IN	denotes	by
T2695	2773-2776	DT	denotes	the
T2697	2777-2784	VBN	denotes	induced
T2698	2785-2796	JJ	denotes	metanephric
T2696	2797-2807	NN	denotes	mesenchyme
T2699	2808-2811	CC	denotes	and
T2700	2812-2814	VBZ	denotes	is
T2702	2815-2819	RB	denotes	also
T2701	2820-2828	VBN	denotes	required
T2703	2829-2832	IN	denotes	for
T2704	2833-2846	NN	denotes	nephrogenesis
T2705	2847-2848	-LRB-	denotes	[
T2706	2848-2849	CD	denotes	3
T2707	2849-2850	-RRB-	denotes	]
T2708	2850-2851	.	denotes	.
T2709	2851-3054	sentence	denotes	Furthermore, Wnt11, secreted by the ureteric bud tips, participates in a positive feedback loop promoting glial cell line-derived neurotrophic factor (GDNF) expression by the metanephric mesenchyme [4].
T2710	2852-2863	RB	denotes	Furthermore
T2712	2863-2865	,	denotes	,
T2713	2865-2870	NN	denotes	Wnt11
T2714	2870-2872	,	denotes	,
T2715	2872-2880	VBN	denotes	secreted
T2716	2881-2883	IN	denotes	by
T2717	2884-2887	DT	denotes	the
T2719	2888-2896	JJ	denotes	ureteric
T2720	2897-2900	NN	denotes	bud
T2718	2901-2905	NNS	denotes	tips
T2721	2905-2907	,	denotes	,
T2711	2907-2919	VBZ	denotes	participates
T2722	2920-2922	IN	denotes	in
T2723	2923-2924	DT	denotes	a
T2725	2925-2933	JJ	denotes	positive
T2726	2934-2942	NN	denotes	feedback
T2724	2943-2947	NN	denotes	loop
T2727	2948-2957	VBG	denotes	promoting
T2728	2958-2963	JJ	denotes	glial
T2730	2964-2968	NN	denotes	cell
T2729	2969-2973	NN	denotes	line
T2732	2973-2974	HYPH	denotes	-
T2731	2974-2981	VBN	denotes	derived
T2734	2982-2994	JJ	denotes	neurotrophic
T2733	2995-3001	NN	denotes	factor
T2736	3002-3003	-LRB-	denotes	(
T2737	3003-3007	NN	denotes	GDNF
T2738	3007-3008	-RRB-	denotes	)
T2735	3009-3019	NN	denotes	expression
T2739	3020-3022	IN	denotes	by
T2740	3023-3026	DT	denotes	the
T2742	3027-3038	JJ	denotes	metanephric
T2741	3039-3049	NN	denotes	mesenchyme
T2743	3050-3051	-LRB-	denotes	[
T2744	3051-3052	CD	denotes	4
T2745	3052-3053	-RRB-	denotes	]
T2746	3053-3054	.	denotes	.
T2747	3054-3198	sentence	denotes	Mutations in Wnt9b or Wnt4 result in a dramatic block in nephron formation, while Wnt11 mutants show a significant reduction in nephron number.
T2748	3055-3064	NNS	denotes	Mutations
T2750	3065-3067	IN	denotes	in
T2751	3068-3073	NN	denotes	Wnt9b
T2752	3074-3076	CC	denotes	or
T2753	3077-3081	NN	denotes	Wnt4
T2749	3082-3088	VBP	denotes	result
T2754	3089-3091	IN	denotes	in
T2755	3092-3093	DT	denotes	a
T2757	3094-3102	JJ	denotes	dramatic
T2756	3103-3108	NN	denotes	block
T2758	3109-3111	IN	denotes	in
T2759	3112-3119	NN	denotes	nephron
T2760	3120-3129	NN	denotes	formation
T2761	3129-3131	,	denotes	,
T2762	3131-3136	IN	denotes	while
T2764	3137-3142	NN	denotes	Wnt11
T2765	3143-3150	NNS	denotes	mutants
T2763	3151-3155	VBP	denotes	show
T2766	3156-3157	DT	denotes	a
T2768	3158-3169	JJ	denotes	significant
T2767	3170-3179	NN	denotes	reduction
T2769	3180-3182	IN	denotes	in
T2770	3183-3190	NN	denotes	nephron
T2771	3191-3197	NN	denotes	number
T2772	3197-3198	.	denotes	.
T2773	3198-3431	sentence	denotes	It is interesting to note that Wnt4 and Wnt11 have been shown to signal, at least in some cases, through noncanonical pathways [5-7], while there is evidence indicating that Wnt9b activates canonical Wnt signaling in the kidney [2].
T2774	3199-3201	PRP	denotes	It
T2775	3202-3204	VBZ	denotes	is
T2776	3205-3216	JJ	denotes	interesting
T2777	3217-3219	TO	denotes	to
T2778	3220-3224	VB	denotes	note
T2779	3225-3229	IN	denotes	that
T2781	3230-3234	NN	denotes	Wnt4
T2782	3235-3238	CC	denotes	and
T2783	3239-3244	NN	denotes	Wnt11
T2784	3245-3249	VBP	denotes	have
T2785	3250-3254	VBN	denotes	been
T2780	3255-3260	VBN	denotes	shown
T2786	3261-3263	TO	denotes	to
T2787	3264-3270	VB	denotes	signal
T2788	3270-3272	,	denotes	,
T2789	3272-3274	RB	denotes	at
T2790	3275-3280	RBS	denotes	least
T2791	3281-3283	IN	denotes	in
T2792	3284-3288	DT	denotes	some
T2793	3289-3294	NNS	denotes	cases
T2794	3294-3296	,	denotes	,
T2795	3296-3303	IN	denotes	through
T2796	3304-3316	JJ	denotes	noncanonical
T2797	3317-3325	NNS	denotes	pathways
T2798	3326-3327	-LRB-	denotes	[
T2799	3327-3328	CD	denotes	5
T2800	3328-3329	SYM	denotes	-
T2801	3329-3330	CD	denotes	7
T2802	3330-3331	-RRB-	denotes	]
T2803	3331-3333	,	denotes	,
T2804	3333-3338	IN	denotes	while
T2806	3339-3344	EX	denotes	there
T2805	3345-3347	VBZ	denotes	is
T2807	3348-3356	NN	denotes	evidence
T2808	3357-3367	VBG	denotes	indicating
T2809	3368-3372	IN	denotes	that
T2811	3373-3378	NN	denotes	Wnt9b
T2810	3379-3388	VBZ	denotes	activates
T2812	3389-3398	JJ	denotes	canonical
T2814	3399-3402	NN	denotes	Wnt
T2813	3403-3412	NN	denotes	signaling
T2815	3413-3415	IN	denotes	in
T2816	3416-3419	DT	denotes	the
T2817	3420-3426	NN	denotes	kidney
T2818	3427-3428	-LRB-	denotes	[
T2819	3428-3429	CD	denotes	2
T2820	3429-3430	-RRB-	denotes	]
T2821	3430-3431	.	denotes	.
T2822	3431-3560	sentence	denotes	Genetic studies in Drosophila have identified the Pygopus (Pygo) gene as a critical component of canonical Wnt signaling [8-11].
T2823	3432-3439	JJ	denotes	Genetic
T2824	3440-3447	NNS	denotes	studies
T2826	3448-3450	IN	denotes	in
T2827	3451-3461	NNP	denotes	Drosophila
T2828	3462-3466	VBP	denotes	have
T2825	3467-3477	VBN	denotes	identified
T2829	3478-3481	DT	denotes	the
T2831	3482-3489	NN	denotes	Pygopus
T2832	3490-3491	-LRB-	denotes	(
T2833	3491-3495	NN	denotes	Pygo
T2834	3495-3496	-RRB-	denotes	)
T2830	3497-3501	NN	denotes	gene
T2835	3502-3504	IN	denotes	as
T2836	3505-3506	DT	denotes	a
T2838	3507-3515	JJ	denotes	critical
T2837	3516-3525	NN	denotes	component
T2839	3526-3528	IN	denotes	of
T2840	3529-3538	JJ	denotes	canonical
T2842	3539-3542	NN	denotes	Wnt
T2841	3543-3552	NN	denotes	signaling
T2843	3553-3554	-LRB-	denotes	[
T2844	3554-3555	CD	denotes	8
T2845	3555-3556	SYM	denotes	-
T2846	3556-3558	CD	denotes	11
T2847	3558-3559	-RRB-	denotes	]
T2848	3559-3560	.	denotes	.
T2849	3560-3727	sentence	denotes	Pygo and Lgs interact with β-catenin during the formation of the canonical transcriptional complex and are required for accumulation of β-catenin in the nucleus [12].
T2850	3561-3565	NN	denotes	Pygo
T2852	3566-3569	CC	denotes	and
T2853	3570-3573	NN	denotes	Lgs
T2851	3574-3582	VBP	denotes	interact
T2854	3583-3587	IN	denotes	with
T2855	3588-3589	NN	denotes	β
T2857	3589-3590	HYPH	denotes	-
T2856	3590-3597	NN	denotes	catenin
T2858	3598-3604	IN	denotes	during
T2859	3605-3608	DT	denotes	the
T2860	3609-3618	NN	denotes	formation
T2861	3619-3621	IN	denotes	of
T2862	3622-3625	DT	denotes	the
T2864	3626-3635	JJ	denotes	canonical
T2865	3636-3651	JJ	denotes	transcriptional
T2863	3652-3659	NN	denotes	complex
T2866	3660-3663	CC	denotes	and
T2867	3664-3667	VBP	denotes	are
T2868	3668-3676	VBN	denotes	required
T2869	3677-3680	IN	denotes	for
T2870	3681-3693	NN	denotes	accumulation
T2871	3694-3696	IN	denotes	of
T2872	3697-3698	NN	denotes	β
T2874	3698-3699	HYPH	denotes	-
T2873	3699-3706	NN	denotes	catenin
T2875	3707-3709	IN	denotes	in
T2876	3710-3713	DT	denotes	the
T2877	3714-3721	NN	denotes	nucleus
T2878	3722-3723	-LRB-	denotes	[
T2879	3723-3725	CD	denotes	12
T2880	3725-3726	-RRB-	denotes	]
T2881	3726-3727	.	denotes	.
T2882	3727-3856	sentence	denotes	Lgs binds the central armadillo repeats of β-catenin, while Pygo interacts with Lgs, mediating activation of Wnt targets [9,13].
T2883	3728-3731	NN	denotes	Lgs
T2884	3732-3737	VBZ	denotes	binds
T2885	3738-3741	DT	denotes	the
T2887	3742-3749	JJ	denotes	central
T2888	3750-3759	NN	denotes	armadillo
T2886	3760-3767	NNS	denotes	repeats
T2889	3768-3770	IN	denotes	of
T2890	3771-3772	NN	denotes	β
T2892	3772-3773	HYPH	denotes	-
T2891	3773-3780	NN	denotes	catenin
T2893	3780-3782	,	denotes	,
T2894	3782-3787	IN	denotes	while
T2896	3788-3792	NN	denotes	Pygo
T2895	3793-3802	VBZ	denotes	interacts
T2897	3803-3807	IN	denotes	with
T2898	3808-3811	NN	denotes	Lgs
T2899	3811-3813	,	denotes	,
T2900	3813-3822	VBG	denotes	mediating
T2901	3823-3833	NN	denotes	activation
T2902	3834-3836	IN	denotes	of
T2903	3837-3840	NN	denotes	Wnt
T2904	3841-3848	NNS	denotes	targets
T2905	3849-3850	-LRB-	denotes	[
T2907	3850-3851	CD	denotes	9
T2908	3851-3852	,	denotes	,
T2906	3852-3854	CD	denotes	13
T2909	3854-3855	-RRB-	denotes	]
T2910	3855-3856	.	denotes	.
T2911	3856-4011	sentence	denotes	The N-terminal domain of Pygo is required for Wnt transcriptional activation, while the PHD motif is required for the association of Pygo with Lgs [9,13].
T2912	3857-3860	DT	denotes	The
T2914	3861-3862	NN	denotes	N
T2916	3862-3863	HYPH	denotes	-
T2915	3863-3871	JJ	denotes	terminal
T2913	3872-3878	NN	denotes	domain
T2918	3879-3881	IN	denotes	of
T2919	3882-3886	NN	denotes	Pygo
T2920	3887-3889	VBZ	denotes	is
T2917	3890-3898	VBN	denotes	required
T2921	3899-3902	IN	denotes	for
T2922	3903-3906	NN	denotes	Wnt
T2924	3907-3922	JJ	denotes	transcriptional
T2923	3923-3933	NN	denotes	activation
T2925	3933-3935	,	denotes	,
T2926	3935-3940	IN	denotes	while
T2928	3941-3944	DT	denotes	the
T2930	3945-3948	NN	denotes	PHD
T2929	3949-3954	NN	denotes	motif
T2931	3955-3957	VBZ	denotes	is
T2927	3958-3966	VBN	denotes	required
T2932	3967-3970	IN	denotes	for
T2933	3971-3974	DT	denotes	the
T2934	3975-3986	NN	denotes	association
T2935	3987-3989	IN	denotes	of
T2936	3990-3994	NN	denotes	Pygo
T2937	3995-3999	IN	denotes	with
T2938	4000-4003	NN	denotes	Lgs
T2939	4004-4005	-LRB-	denotes	[
T2941	4005-4006	CD	denotes	9
T2942	4006-4007	,	denotes	,
T2940	4007-4009	CD	denotes	13
T2943	4009-4010	-RRB-	denotes	]
T2944	4010-4011	.	denotes	.
T2945	4011-4194	sentence	denotes	Additionally, a putative nuclear localization signal (NLS) was identified within the N-terminal domain of Pygo, suggesting a possible role of nuclear importation of β-catenin [8,11].
T2946	4012-4024	RB	denotes	Additionally
T2948	4024-4026	,	denotes	,
T2949	4026-4027	DT	denotes	a
T2951	4028-4036	JJ	denotes	putative
T2952	4037-4044	JJ	denotes	nuclear
T2953	4045-4057	NN	denotes	localization
T2950	4058-4064	NN	denotes	signal
T2954	4065-4066	-LRB-	denotes	(
T2955	4066-4069	NN	denotes	NLS
T2956	4069-4070	-RRB-	denotes	)
T2957	4071-4074	VBD	denotes	was
T2947	4075-4085	VBN	denotes	identified
T2958	4086-4092	IN	denotes	within
T2959	4093-4096	DT	denotes	the
T2961	4097-4098	NN	denotes	N
T2963	4098-4099	HYPH	denotes	-
T2962	4099-4107	JJ	denotes	terminal
T2960	4108-4114	NN	denotes	domain
T2964	4115-4117	IN	denotes	of
T2965	4118-4122	NN	denotes	Pygo
T2966	4122-4124	,	denotes	,
T2967	4124-4134	VBG	denotes	suggesting
T2968	4135-4136	DT	denotes	a
T2970	4137-4145	JJ	denotes	possible
T2969	4146-4150	NN	denotes	role
T2971	4151-4153	IN	denotes	of
T2972	4154-4161	JJ	denotes	nuclear
T2973	4162-4173	NN	denotes	importation
T2974	4174-4176	IN	denotes	of
T2975	4177-4178	NN	denotes	β
T2977	4178-4179	HYPH	denotes	-
T2976	4179-4186	NN	denotes	catenin
T2978	4187-4188	-LRB-	denotes	[
T2980	4188-4189	CD	denotes	8
T2981	4189-4190	,	denotes	,
T2979	4190-4192	CD	denotes	11
T2982	4192-4193	-RRB-	denotes	]
T2983	4193-4194	.	denotes	.
T2984	4194-4373	sentence	denotes	Analyses of multiple aspects of the pygopus mutant phenotype indicate that this gene is dedicated to, and required for, canonical Wnt signaling during Drosophila development [9].
T2985	4195-4203	NNS	denotes	Analyses
T2987	4204-4206	IN	denotes	of
T2988	4207-4215	JJ	denotes	multiple
T2989	4216-4223	NNS	denotes	aspects
T2990	4224-4226	IN	denotes	of
T2991	4227-4230	DT	denotes	the
T2993	4231-4238	NN	denotes	pygopus
T2994	4239-4245	NN	denotes	mutant
T2992	4246-4255	NN	denotes	phenotype
T2986	4256-4264	VBP	denotes	indicate
T2995	4265-4269	IN	denotes	that
T2997	4270-4274	DT	denotes	this
T2998	4275-4279	NN	denotes	gene
T2999	4280-4282	VBZ	denotes	is
T2996	4283-4292	VBN	denotes	dedicated
T3000	4293-4295	IN	denotes	to
T3001	4295-4297	,	denotes	,
T3002	4297-4300	CC	denotes	and
T3003	4301-4309	VBN	denotes	required
T3004	4310-4313	IN	denotes	for
T3005	4313-4315	,	denotes	,
T3006	4315-4324	JJ	denotes	canonical
T3008	4325-4328	NN	denotes	Wnt
T3007	4329-4338	NN	denotes	signaling
T3009	4339-4345	IN	denotes	during
T3010	4346-4356	NNP	denotes	Drosophila
T3011	4357-4368	NN	denotes	development
T3012	4369-4370	-LRB-	denotes	[
T3013	4370-4371	CD	denotes	9
T3014	4371-4372	-RRB-	denotes	]
T3015	4372-4373	.	denotes	.
T3016	4373-4477	sentence	denotes	The mammalian genome carries two, and only two, orthologs of Drosophila Pygo, Pygo1 and Pygo2 [8,9,14].
T3017	4374-4377	DT	denotes	The
T3019	4378-4387	JJ	denotes	mammalian
T3018	4388-4394	NN	denotes	genome
T3020	4395-4402	VBZ	denotes	carries
T3021	4403-4406	CD	denotes	two
T3023	4406-4408	,	denotes	,
T3024	4408-4411	CC	denotes	and
T3025	4412-4416	RB	denotes	only
T3026	4417-4420	CD	denotes	two
T3027	4420-4422	,	denotes	,
T3022	4422-4431	NNS	denotes	orthologs
T3028	4432-4434	IN	denotes	of
T3029	4435-4445	NNP	denotes	Drosophila
T3030	4446-4450	NN	denotes	Pygo
T3031	4450-4452	,	denotes	,
T3032	4452-4457	NN	denotes	Pygo1
T3033	4458-4461	CC	denotes	and
T3034	4462-4467	NN	denotes	Pygo2
T3035	4468-4469	-LRB-	denotes	[
T3037	4469-4470	CD	denotes	8
T3038	4470-4471	,	denotes	,
T3039	4471-4472	CD	denotes	9
T3040	4472-4473	,	denotes	,
T3036	4473-4475	CD	denotes	14
T3041	4475-4476	-RRB-	denotes	]
T3042	4476-4477	.	denotes	.
T3043	4477-4691	sentence	denotes	In this report, we generated targeted mutations of Pygo1 and Pygo2 to determine their functions, with a particular interest in the contributions of these genes to canonical Wnt signaling during kidney development.
T3044	4478-4480	IN	denotes	In
T3046	4481-4485	DT	denotes	this
T3047	4486-4492	NN	denotes	report
T3048	4492-4494	,	denotes	,
T3049	4494-4496	PRP	denotes	we
T3045	4497-4506	VBD	denotes	generated
T3050	4507-4515	VBN	denotes	targeted
T3051	4516-4525	NNS	denotes	mutations
T3052	4526-4528	IN	denotes	of
T3053	4529-4534	NN	denotes	Pygo1
T3054	4535-4538	CC	denotes	and
T3055	4539-4544	NN	denotes	Pygo2
T3056	4545-4547	TO	denotes	to
T3057	4548-4557	VB	denotes	determine
T3058	4558-4563	PRP$	denotes	their
T3059	4564-4573	NNS	denotes	functions
T3060	4573-4575	,	denotes	,
T3061	4575-4579	IN	denotes	with
T3062	4580-4581	DT	denotes	a
T3064	4582-4592	JJ	denotes	particular
T3063	4593-4601	NN	denotes	interest
T3065	4602-4604	IN	denotes	in
T3066	4605-4608	DT	denotes	the
T3067	4609-4622	NNS	denotes	contributions
T3068	4623-4625	IN	denotes	of
T3069	4626-4631	DT	denotes	these
T3070	4632-4637	NNS	denotes	genes
T3071	4638-4640	IN	denotes	to
T3072	4641-4650	JJ	denotes	canonical
T3074	4651-4654	NN	denotes	Wnt
T3073	4655-4664	NN	denotes	signaling
T3075	4665-4671	IN	denotes	during
T3076	4672-4678	NN	denotes	kidney
T3077	4679-4690	NN	denotes	development
T3078	4690-4691	.	denotes	.
T3079	4691-4852	sentence	denotes	The resulting double-homozygous mutant embryos showed a context-dependent reduction in canonical Wnt signaling as measured by Wnt reporter transgene expression.
T3080	4692-4695	DT	denotes	The
T3082	4696-4705	VBG	denotes	resulting
T3083	4706-4712	JJ	denotes	double
T3085	4712-4713	HYPH	denotes	-
T3084	4713-4723	JJ	denotes	homozygous
T3086	4724-4730	NN	denotes	mutant
T3081	4731-4738	NNS	denotes	embryos
T3087	4739-4745	VBD	denotes	showed
T3088	4746-4747	DT	denotes	a
T3090	4748-4755	NN	denotes	context
T3092	4755-4756	HYPH	denotes	-
T3091	4756-4765	JJ	denotes	dependent
T3089	4766-4775	NN	denotes	reduction
T3093	4776-4778	IN	denotes	in
T3094	4779-4788	JJ	denotes	canonical
T3096	4789-4792	NN	denotes	Wnt
T3095	4793-4802	NN	denotes	signaling
T3097	4803-4805	IN	denotes	as
T3098	4806-4814	VBN	denotes	measured
T3099	4815-4817	IN	denotes	by
T3100	4818-4821	NN	denotes	Wnt
T3101	4822-4830	NN	denotes	reporter
T3102	4831-4840	NN	denotes	transgene
T3103	4841-4851	NN	denotes	expression
T3104	4851-4852	.	denotes	.
T3105	4852-4975	sentence	denotes	Development remained, however, surprisingly normal, with survival to birth and few apparent defects in most organ systems.
T3106	4853-4864	NN	denotes	Development
T3107	4865-4873	VBD	denotes	remained
T3108	4873-4875	,	denotes	,
T3109	4875-4882	RB	denotes	however
T3110	4882-4884	,	denotes	,
T3111	4884-4896	RB	denotes	surprisingly
T3112	4897-4903	JJ	denotes	normal
T3113	4903-4905	,	denotes	,
T3114	4905-4909	IN	denotes	with
T3115	4910-4918	NN	denotes	survival
T3116	4919-4921	IN	denotes	to
T3117	4922-4927	NN	denotes	birth
T3118	4928-4931	CC	denotes	and
T3119	4932-4935	JJ	denotes	few
T3121	4936-4944	JJ	denotes	apparent
T3120	4945-4952	NNS	denotes	defects
T3122	4953-4955	IN	denotes	in
T3123	4956-4960	JJS	denotes	most
T3125	4961-4966	NN	denotes	organ
T3124	4967-4974	NNS	denotes	systems
T3126	4974-4975	.	denotes	.
T3127	4975-5301	sentence	denotes	Our phenotypic analysis focused on the kidney, which showed altered branching morphogenesis of the ureteric bud, and expansion of the zone of condensed mesenchyme surrounding the ureteric bud, yet relatively normal nephron formation, as measured by histology, confocal analysis, in situ hybridization and microarray analysis.
T3128	4976-4979	PRP$	denotes	Our
T3130	4980-4990	JJ	denotes	phenotypic
T3129	4991-4999	NN	denotes	analysis
T3131	5000-5007	VBD	denotes	focused
T3132	5008-5010	IN	denotes	on
T3133	5011-5014	DT	denotes	the
T3134	5015-5021	NN	denotes	kidney
T3135	5021-5023	,	denotes	,
T3136	5023-5028	WDT	denotes	which
T3137	5029-5035	VBD	denotes	showed
T3138	5036-5043	VBN	denotes	altered
T3140	5044-5053	NN	denotes	branching
T3139	5054-5067	NN	denotes	morphogenesis
T3141	5068-5070	IN	denotes	of
T3142	5071-5074	DT	denotes	the
T3144	5075-5083	JJ	denotes	ureteric
T3143	5084-5087	NN	denotes	bud
T3145	5087-5089	,	denotes	,
T3146	5089-5092	CC	denotes	and
T3147	5093-5102	NN	denotes	expansion
T3148	5103-5105	IN	denotes	of
T3149	5106-5109	DT	denotes	the
T3150	5110-5114	NN	denotes	zone
T3151	5115-5117	IN	denotes	of
T3152	5118-5127	VBN	denotes	condensed
T3153	5128-5138	NN	denotes	mesenchyme
T3154	5139-5150	VBG	denotes	surrounding
T3155	5151-5154	DT	denotes	the
T3157	5155-5163	JJ	denotes	ureteric
T3156	5164-5167	NN	denotes	bud
T3158	5167-5169	,	denotes	,
T3159	5169-5172	CC	denotes	yet
T3160	5173-5183	RB	denotes	relatively
T3161	5184-5190	JJ	denotes	normal
T3163	5191-5198	NN	denotes	nephron
T3162	5199-5208	NN	denotes	formation
T3164	5208-5210	,	denotes	,
T3165	5210-5212	IN	denotes	as
T3166	5213-5221	VBN	denotes	measured
T3167	5222-5224	IN	denotes	by
T3168	5225-5234	NN	denotes	histology
T3169	5234-5236	,	denotes	,
T3170	5236-5244	JJ	denotes	confocal
T3171	5245-5253	NN	denotes	analysis
T3172	5253-5255	,	denotes	,
T3173	5255-5257	FW	denotes	in
T3174	5258-5262	FW	denotes	situ
T3175	5263-5276	NN	denotes	hybridization
T3176	5277-5280	CC	denotes	and
T3177	5281-5291	NN	denotes	microarray
T3178	5292-5300	NN	denotes	analysis
T3179	5300-5301	.	denotes	.
T3180	5301-5466	sentence	denotes	The obvious conclusion is that in mammals, unlike Drosophila, Pygopus-mediated canonical Wnt signaling is not absolutely necessary in most developing organ systems.
T3181	5302-5305	DT	denotes	The
T3183	5306-5313	JJ	denotes	obvious
T3182	5314-5324	NN	denotes	conclusion
T3184	5325-5327	VBZ	denotes	is
T3185	5328-5332	IN	denotes	that
T3187	5333-5335	IN	denotes	in
T3188	5336-5343	NNS	denotes	mammals
T3189	5343-5345	,	denotes	,
T3190	5345-5351	IN	denotes	unlike
T3191	5352-5362	NNP	denotes	Drosophila
T3192	5362-5364	,	denotes	,
T3193	5364-5371	NN	denotes	Pygopus
T3195	5371-5372	HYPH	denotes	-
T3194	5372-5380	VBN	denotes	mediated
T3197	5381-5390	JJ	denotes	canonical
T3198	5391-5394	NN	denotes	Wnt
T3196	5395-5404	NN	denotes	signaling
T3186	5405-5407	VBZ	denotes	is
T3199	5408-5411	RB	denotes	not
T3200	5412-5422	RB	denotes	absolutely
T3201	5423-5432	JJ	denotes	necessary
T3202	5433-5435	IN	denotes	in
T3203	5436-5440	JJS	denotes	most
T3205	5441-5451	VBG	denotes	developing
T3206	5452-5457	NN	denotes	organ
T3204	5458-5465	NNS	denotes	systems
T3207	5465-5466	.	denotes	.
T3498	5477-5482	NN	denotes	Pygo1
T3500	5483-5486	CC	denotes	and
T3501	5487-5492	NN	denotes	Pygo2
T3499	5493-5501	VBN	denotes	targeted
T3502	5502-5511	NNS	denotes	mutations
T3503	5511-5642	sentence	denotes	We targeted both the Pygo1 and Pygo2 genes by inserting LoxP sequences to flank critical coding regions including the PHD domains.
T3504	5512-5514	PRP	denotes	We
T3505	5515-5523	VBD	denotes	targeted
T3506	5524-5528	CC	denotes	both
T3508	5529-5532	DT	denotes	the
T3509	5533-5538	NN	denotes	Pygo1
T3510	5539-5542	CC	denotes	and
T3511	5543-5548	NN	denotes	Pygo2
T3507	5549-5554	NNS	denotes	genes
T3512	5555-5557	IN	denotes	by
T3513	5558-5567	VBG	denotes	inserting
T3514	5568-5572	NN	denotes	LoxP
T3515	5573-5582	NNS	denotes	sequences
T3516	5583-5585	TO	denotes	to
T3517	5586-5591	VB	denotes	flank
T3518	5592-5600	JJ	denotes	critical
T3520	5601-5607	VBG	denotes	coding
T3519	5608-5615	NNS	denotes	regions
T3521	5616-5625	VBG	denotes	including
T3522	5626-5629	DT	denotes	the
T3524	5630-5633	NN	denotes	PHD
T3523	5634-5641	NNS	denotes	domains
T3525	5641-5642	.	denotes	.
T3526	5642-5821	sentence	denotes	The resulting targeted mice were mated with transgenic CMV-Cre mice [15] to drive germline LoxP recombination, resulting in the null mutant alleles that were used for this study.
T3527	5643-5646	DT	denotes	The
T3529	5647-5656	VBG	denotes	resulting
T3530	5657-5665	VBN	denotes	targeted
T3528	5666-5670	NNS	denotes	mice
T3532	5671-5675	VBD	denotes	were
T3531	5676-5681	VBN	denotes	mated
T3533	5682-5686	IN	denotes	with
T3534	5687-5697	JJ	denotes	transgenic
T3536	5698-5701	NN	denotes	CMV
T3538	5701-5702	HYPH	denotes	-
T3537	5702-5705	NN	denotes	Cre
T3535	5706-5710	NNS	denotes	mice
T3539	5711-5712	-LRB-	denotes	[
T3540	5712-5714	CD	denotes	15
T3541	5714-5715	-RRB-	denotes	]
T3542	5716-5718	TO	denotes	to
T3543	5719-5724	VB	denotes	drive
T3544	5725-5733	NN	denotes	germline
T3545	5734-5738	NN	denotes	LoxP
T3546	5739-5752	NN	denotes	recombination
T3547	5752-5754	,	denotes	,
T3548	5754-5763	VBG	denotes	resulting
T3549	5764-5766	IN	denotes	in
T3550	5767-5770	DT	denotes	the
T3552	5771-5775	JJ	denotes	null
T3553	5776-5782	NN	denotes	mutant
T3551	5783-5790	NNS	denotes	alleles
T3554	5791-5795	WDT	denotes	that
T3556	5796-5800	VBD	denotes	were
T3555	5801-5805	VBN	denotes	used
T3557	5806-5809	IN	denotes	for
T3558	5810-5814	DT	denotes	this
T3559	5815-5820	NN	denotes	study
T3560	5820-5821	.	denotes	.
T3561	5821-5981	sentence	denotes	PCR confirmed the deletion of the bulk of the coding sequences for both genes, including 89% of coding sequence for Pygo1 and 87% of coding sequence for Pygo2.
T3562	5822-5825	NN	denotes	PCR
T3563	5826-5835	VBD	denotes	confirmed
T3564	5836-5839	DT	denotes	the
T3565	5840-5848	NN	denotes	deletion
T3566	5849-5851	IN	denotes	of
T3567	5852-5855	DT	denotes	the
T3568	5856-5860	NN	denotes	bulk
T3569	5861-5863	IN	denotes	of
T3570	5864-5867	DT	denotes	the
T3572	5868-5874	VBG	denotes	coding
T3571	5875-5884	NNS	denotes	sequences
T3573	5885-5888	IN	denotes	for
T3574	5889-5893	DT	denotes	both
T3575	5894-5899	NNS	denotes	genes
T3576	5899-5901	,	denotes	,
T3577	5901-5910	VBG	denotes	including
T3578	5911-5913	CD	denotes	89
T3579	5913-5914	NN	denotes	%
T3580	5915-5917	IN	denotes	of
T3581	5918-5924	NN	denotes	coding
T3582	5925-5933	NN	denotes	sequence
T3583	5934-5937	IN	denotes	for
T3584	5938-5943	NN	denotes	Pygo1
T3585	5944-5947	CC	denotes	and
T3586	5948-5950	CD	denotes	87
T3587	5950-5951	NN	denotes	%
T3588	5952-5954	IN	denotes	of
T3589	5955-5961	NN	denotes	coding
T3590	5962-5970	NN	denotes	sequence
T3591	5971-5974	IN	denotes	for
T3592	5975-5980	NN	denotes	Pygo2
T3593	5980-5981	.	denotes	.
T3594	5981-6128	sentence	denotes	Previous studies in Drosophila have shown that even a single missense mutation in the PHD domain can eliminate Pygo function in Wnt signaling [8].
T3595	5982-5990	JJ	denotes	Previous
T3596	5991-5998	NNS	denotes	studies
T3598	5999-6001	IN	denotes	in
T3599	6002-6012	NNP	denotes	Drosophila
T3600	6013-6017	VBP	denotes	have
T3597	6018-6023	VBN	denotes	shown
T3601	6024-6028	IN	denotes	that
T3603	6029-6033	RB	denotes	even
T3605	6034-6035	DT	denotes	a
T3606	6036-6042	JJ	denotes	single
T3607	6043-6051	NN	denotes	missense
T3604	6052-6060	NN	denotes	mutation
T3608	6061-6063	IN	denotes	in
T3609	6064-6067	DT	denotes	the
T3611	6068-6071	NN	denotes	PHD
T3610	6072-6078	NN	denotes	domain
T3612	6079-6082	MD	denotes	can
T3602	6083-6092	VB	denotes	eliminate
T3613	6093-6097	NN	denotes	Pygo
T3614	6098-6106	NN	denotes	function
T3615	6107-6109	IN	denotes	in
T3616	6110-6113	NN	denotes	Wnt
T3617	6114-6123	NN	denotes	signaling
T3618	6124-6125	-LRB-	denotes	[
T3619	6125-6126	CD	denotes	8
T3620	6126-6127	-RRB-	denotes	]
T3621	6127-6128	.	denotes	.
T4091	6130-6135	NN	denotes	Pygo1
T4093	6136-6139	CC	denotes	and
T4094	6140-6145	NN	denotes	Pygo2
T4092	6146-6152	NN	denotes	mutant
T4095	6153-6163	NNS	denotes	phenotypes
T4096	6163-6256	sentence	denotes	Pygo1 homozygous null mice were viable and fertile with no developmental defects detectable.
T4097	6164-6169	NN	denotes	Pygo1
T4099	6170-6180	JJ	denotes	homozygous
T4098	6181-6185	JJ	denotes	null
T4100	6186-6190	NNS	denotes	mice
T4101	6191-6195	VBD	denotes	were
T4102	6196-6202	JJ	denotes	viable
T4103	6203-6206	CC	denotes	and
T4104	6207-6214	JJ	denotes	fertile
T4105	6215-6219	IN	denotes	with
T4107	6220-6222	DT	denotes	no
T4109	6223-6236	JJ	denotes	developmental
T4108	6237-6244	NNS	denotes	defects
T4106	6245-6255	JJ	denotes	detectable
T4110	6255-6256	.	denotes	.
T4111	6256-6550	sentence	denotes	This was surprising given the importance of the pygopus gene in Wnt signaling in Drosophila, and the reported expression during development of the mouse Pygo1 gene in, for example, the brain, limbs, kidney and branchial arches [14,16] (Yu J, Valerius MT, McMahon AP, contribution to GUDMAP, ).
T4112	6257-6261	DT	denotes	This
T4113	6262-6265	VBD	denotes	was
T4114	6266-6276	JJ	denotes	surprising
T4115	6277-6282	VBN	denotes	given
T4116	6283-6286	DT	denotes	the
T4117	6287-6297	NN	denotes	importance
T4118	6298-6300	IN	denotes	of
T4119	6301-6304	DT	denotes	the
T4121	6305-6312	NN	denotes	pygopus
T4120	6313-6317	NN	denotes	gene
T4122	6318-6320	IN	denotes	in
T4123	6321-6324	NN	denotes	Wnt
T4124	6325-6334	NN	denotes	signaling
T4125	6335-6337	IN	denotes	in
T4126	6338-6348	NNP	denotes	Drosophila
T4127	6348-6350	,	denotes	,
T4128	6350-6353	CC	denotes	and
T4129	6354-6357	DT	denotes	the
T4131	6358-6366	VBN	denotes	reported
T4130	6367-6377	NN	denotes	expression
T4132	6378-6384	IN	denotes	during
T4133	6385-6396	NN	denotes	development
T4134	6397-6399	IN	denotes	of
T4135	6400-6403	DT	denotes	the
T4137	6404-6409	NN	denotes	mouse
T4138	6410-6415	NN	denotes	Pygo1
T4136	6416-6420	NN	denotes	gene
T4139	6421-6423	IN	denotes	in
T4140	6423-6425	,	denotes	,
T4141	6425-6428	IN	denotes	for
T4142	6429-6436	NN	denotes	example
T4143	6436-6438	,	denotes	,
T4144	6438-6441	DT	denotes	the
T4145	6442-6447	NN	denotes	brain
T4146	6447-6449	,	denotes	,
T4147	6449-6454	NNS	denotes	limbs
T4148	6454-6456	,	denotes	,
T4149	6456-6462	NN	denotes	kidney
T4150	6463-6466	CC	denotes	and
T4151	6467-6476	JJ	denotes	branchial
T4152	6477-6483	NNS	denotes	arches
T4153	6484-6485	-LRB-	denotes	[
T4155	6485-6487	CD	denotes	14
T4156	6487-6488	,	denotes	,
T4154	6488-6490	CD	denotes	16
T4157	6490-6491	-RRB-	denotes	]
T4158	6492-6493	-LRB-	denotes	(
T4159	6493-6495	NNP	denotes	Yu
T4160	6496-6497	NNP	denotes	J
T4161	6497-6499	,	denotes	,
T4162	6499-6507	NNP	denotes	Valerius
T4163	6508-6510	NNP	denotes	MT
T4164	6510-6512	,	denotes	,
T4165	6512-6519	NNP	denotes	McMahon
T4166	6520-6522	NNP	denotes	AP
T4167	6522-6524	,	denotes	,
T4168	6524-6536	NN	denotes	contribution
T4169	6537-6539	IN	denotes	to
T4170	6540-6546	NN	denotes	GUDMAP
T4171	6546-6548	,	denotes	,
T4172	6548-6549	-RRB-	denotes	)
T4173	6549-6550	.	denotes	.
T4174	6550-6651	sentence	denotes	The Pygo2 homozygous null mice survived to birth, but with rare exceptions, died shortly afterwards.
T4175	6551-6554	DT	denotes	The
T4177	6555-6560	NN	denotes	Pygo2
T4179	6561-6571	JJ	denotes	homozygous
T4178	6572-6576	JJ	denotes	null
T4176	6577-6581	NNS	denotes	mice
T4180	6582-6590	VBD	denotes	survived
T4181	6591-6593	IN	denotes	to
T4182	6594-6599	NN	denotes	birth
T4183	6599-6601	,	denotes	,
T4184	6601-6604	CC	denotes	but
T4185	6605-6609	IN	denotes	with
T4187	6610-6614	JJ	denotes	rare
T4188	6615-6625	NNS	denotes	exceptions
T4189	6625-6627	,	denotes	,
T4186	6627-6631	VBD	denotes	died
T4190	6632-6639	RB	denotes	shortly
T4191	6640-6650	RB	denotes	afterwards
T4192	6650-6651	.	denotes	.
T4193	6651-6780	sentence	denotes	The gut, heart and limbs developed without detectable abnormality (data not shown) despite known requirements for Wnt signaling.
T4194	6652-6655	DT	denotes	The
T4195	6656-6659	NN	denotes	gut
T4197	6659-6661	,	denotes	,
T4198	6661-6666	NN	denotes	heart
T4199	6667-6670	CC	denotes	and
T4200	6671-6676	NNS	denotes	limbs
T4196	6677-6686	VBD	denotes	developed
T4201	6687-6694	IN	denotes	without
T4202	6695-6705	JJ	denotes	detectable
T4203	6706-6717	NN	denotes	abnormality
T4204	6718-6719	-LRB-	denotes	(
T4206	6719-6723	NNS	denotes	data
T4207	6724-6727	RB	denotes	not
T4205	6728-6733	VBN	denotes	shown
T4208	6733-6734	-RRB-	denotes	)
T4209	6735-6742	IN	denotes	despite
T4210	6743-6748	JJ	denotes	known
T4211	6749-6761	NNS	denotes	requirements
T4212	6762-6765	IN	denotes	for
T4213	6766-6769	NN	denotes	Wnt
T4214	6770-6779	NN	denotes	signaling
T4215	6779-6780	.	denotes	.
T4216	6780-6954	sentence	denotes	The Pygo2 mutants did, however, show growth retardation, lens agenesis and a kidney phenotype with high penetrance, exencephaly, and cleft palate with incomplete penetrance.
T4217	6781-6784	DT	denotes	The
T4219	6785-6790	NN	denotes	Pygo2
T4218	6791-6798	NNS	denotes	mutants
T4221	6799-6802	VBD	denotes	did
T4222	6802-6804	,	denotes	,
T4223	6804-6811	RB	denotes	however
T4224	6811-6813	,	denotes	,
T4220	6813-6817	VB	denotes	show
T4225	6818-6824	NN	denotes	growth
T4226	6825-6836	NN	denotes	retardation
T4227	6836-6838	,	denotes	,
T4228	6838-6842	NN	denotes	lens
T4229	6843-6851	NN	denotes	agenesis
T4230	6852-6855	CC	denotes	and
T4231	6856-6857	DT	denotes	a
T4233	6858-6864	NN	denotes	kidney
T4232	6865-6874	NN	denotes	phenotype
T4234	6875-6879	IN	denotes	with
T4235	6880-6884	JJ	denotes	high
T4236	6885-6895	NN	denotes	penetrance
T4237	6895-6897	,	denotes	,
T4238	6897-6908	NN	denotes	exencephaly
T4239	6908-6910	,	denotes	,
T4240	6910-6913	CC	denotes	and
T4241	6914-6919	VBN	denotes	cleft
T4242	6920-6926	NN	denotes	palate
T4243	6927-6931	IN	denotes	with
T4244	6932-6942	JJ	denotes	incomplete
T4245	6943-6953	NN	denotes	penetrance
T4246	6953-6954	.	denotes	.
T4247	6954-7075	sentence	denotes	Double-homozygous mutant Pygo1 and Pygo2 mice had a phenotype similar to that of single Pygo2 nulls (Figure 1, Table 1).
T4248	6955-6961	JJ	denotes	Double
T4250	6961-6962	HYPH	denotes	-
T4249	6962-6972	JJ	denotes	homozygous
T4252	6973-6979	NN	denotes	mutant
T4253	6980-6985	NN	denotes	Pygo1
T4254	6986-6989	CC	denotes	and
T4255	6990-6995	NN	denotes	Pygo2
T4251	6996-7000	NNS	denotes	mice
T4256	7001-7004	VBD	denotes	had
T4257	7005-7006	DT	denotes	a
T4258	7007-7016	NN	denotes	phenotype
T4259	7017-7024	JJ	denotes	similar
T4260	7025-7027	IN	denotes	to
T4261	7028-7032	DT	denotes	that
T4262	7033-7035	IN	denotes	of
T4263	7036-7042	JJ	denotes	single
T4265	7043-7048	NN	denotes	Pygo2
T4264	7049-7054	NNS	denotes	nulls
T4266	7055-7056	-LRB-	denotes	(
T4268	7056-7062	NN	denotes	Figure
T4269	7063-7064	CD	denotes	1
T4270	7064-7066	,	denotes	,
T4267	7066-7071	NN	denotes	Table
T4271	7072-7073	CD	denotes	1
T4272	7073-7074	-RRB-	denotes	)
T4273	7074-7075	.	denotes	.
T4274	7075-7164	sentence	denotes	There was no significant synergism of developmental abnormalities in the double mutants.
T4275	7076-7081	EX	denotes	There
T4276	7082-7085	VBD	denotes	was
T4277	7086-7088	DT	denotes	no
T4279	7089-7100	JJ	denotes	significant
T4278	7101-7110	NN	denotes	synergism
T4280	7111-7113	IN	denotes	of
T4281	7114-7127	JJ	denotes	developmental
T4282	7128-7141	NNS	denotes	abnormalities
T4283	7142-7144	IN	denotes	in
T4284	7145-7148	DT	denotes	the
T4286	7149-7155	JJ	denotes	double
T4285	7156-7163	NNS	denotes	mutants
T4287	7163-7164	.	denotes	.
T4288	7164-7335	sentence	denotes	Together these results suggest that Pygo2 is required for the proper development of a limited number of structures, whereas Pygo1 is not necessary for normal development.
T4289	7165-7173	RB	denotes	Together
T4291	7174-7179	DT	denotes	these
T4292	7180-7187	NNS	denotes	results
T4290	7188-7195	VBP	denotes	suggest
T4293	7196-7200	IN	denotes	that
T4295	7201-7206	NN	denotes	Pygo2
T4296	7207-7209	VBZ	denotes	is
T4294	7210-7218	VBN	denotes	required
T4297	7219-7222	IN	denotes	for
T4298	7223-7226	DT	denotes	the
T4300	7227-7233	JJ	denotes	proper
T4299	7234-7245	NN	denotes	development
T4301	7246-7248	IN	denotes	of
T4302	7249-7250	DT	denotes	a
T4304	7251-7258	JJ	denotes	limited
T4303	7259-7265	NN	denotes	number
T4305	7266-7268	IN	denotes	of
T4306	7269-7279	NNS	denotes	structures
T4307	7279-7281	,	denotes	,
T4308	7281-7288	IN	denotes	whereas
T4310	7289-7294	NN	denotes	Pygo1
T4309	7295-7297	VBZ	denotes	is
T4311	7298-7301	RB	denotes	not
T4312	7302-7311	JJ	denotes	necessary
T4313	7312-7315	IN	denotes	for
T4314	7316-7322	JJ	denotes	normal
T4315	7323-7334	NN	denotes	development
T4316	7334-7335	.	denotes	.
T22293	7346-7351	NN	denotes	Pygo1
T22295	7351-7352	HYPH	denotes	/
T22294	7352-7357	NN	denotes	Pygo2
T22296	7358-7364	NN	denotes	mutant
T22297	7365-7372	NNS	denotes	embryos
T22298	7372-7373	.	denotes	.
T22299	7373-7465	sentence	denotes	(A) E18.5 Pygo1-/-/Pygo2+/- embryos, with only one wild-type Pygo2 allele, appeared normal.
T22300	7374-7375	-LRB-	denotes	(
T22301	7375-7376	LS	denotes	A
T22303	7376-7377	-RRB-	denotes	)
T22304	7378-7383	NN	denotes	E18.5
T22306	7384-7389	NN	denotes	Pygo1
T22307	7389-7390	SYM	denotes	-
T22308	7390-7391	HYPH	denotes	/
T22309	7391-7392	SYM	denotes	-
T22310	7392-7393	HYPH	denotes	/
T22311	7393-7398	NN	denotes	Pygo2
T22312	7398-7399	SYM	denotes	+
T22313	7399-7400	HYPH	denotes	/
T22314	7400-7401	SYM	denotes	-
T22305	7402-7409	NNS	denotes	embryos
T22315	7409-7411	,	denotes	,
T22316	7411-7415	IN	denotes	with
T22317	7416-7420	RB	denotes	only
T22319	7421-7424	CD	denotes	one
T22320	7425-7429	JJ	denotes	wild
T22322	7429-7430	HYPH	denotes	-
T22321	7430-7434	NN	denotes	type
T22323	7435-7440	NN	denotes	Pygo2
T22318	7441-7447	NN	denotes	allele
T22324	7447-7449	,	denotes	,
T22302	7449-7457	VBD	denotes	appeared
T22325	7458-7464	JJ	denotes	normal
T22326	7464-7465	.	denotes	.
T22327	7465-7629	sentence	denotes	(B, C) Double homozygous mutant Pygo1-/-/Pygo2-/- embryos were smaller, with eye defects including absent or rudimentary lens and folded pigmented retina (arrows).
T22328	7466-7467	-LRB-	denotes	(
T22329	7467-7468	LS	denotes	B
T22331	7468-7470	,	denotes	,
T22332	7470-7471	LS	denotes	C
T22333	7471-7472	-RRB-	denotes	)
T22334	7473-7479	JJ	denotes	Double
T22335	7480-7490	JJ	denotes	homozygous
T22336	7491-7497	NN	denotes	mutant
T22338	7498-7503	NN	denotes	Pygo1
T22339	7503-7504	SYM	denotes	-
T22340	7504-7505	HYPH	denotes	/
T22341	7505-7506	SYM	denotes	-
T22342	7506-7507	HYPH	denotes	/
T22343	7507-7512	NN	denotes	Pygo2
T22344	7512-7513	SYM	denotes	-
T22345	7513-7514	HYPH	denotes	/
T22346	7514-7515	SYM	denotes	-
T22337	7516-7523	NNS	denotes	embryos
T22330	7524-7528	VBD	denotes	were
T22347	7529-7536	JJR	denotes	smaller
T22348	7536-7538	,	denotes	,
T22349	7538-7542	IN	denotes	with
T22350	7543-7546	NN	denotes	eye
T22351	7547-7554	NNS	denotes	defects
T22352	7555-7564	VBG	denotes	including
T22353	7565-7571	JJ	denotes	absent
T22355	7572-7574	CC	denotes	or
T22356	7575-7586	JJ	denotes	rudimentary
T22354	7587-7591	NN	denotes	lens
T22357	7592-7595	CC	denotes	and
T22358	7596-7602	VBN	denotes	folded
T22360	7603-7612	VBN	denotes	pigmented
T22359	7613-7619	NN	denotes	retina
T22361	7620-7621	-LRB-	denotes	(
T22362	7621-7627	NNS	denotes	arrows
T22363	7627-7628	-RRB-	denotes	)
T22364	7628-7629	.	denotes	.
T22365	7629-7708	sentence	denotes	(C) A small percentage of Pygo1/Pygo2 null embryos also displayed exencephaly.
T22366	7630-7631	-LRB-	denotes	(
T22367	7631-7632	LS	denotes	C
T22369	7632-7633	-RRB-	denotes	)
T22370	7634-7635	DT	denotes	A
T22372	7636-7641	JJ	denotes	small
T22371	7642-7652	NN	denotes	percentage
T22373	7653-7655	IN	denotes	of
T22374	7656-7661	NN	denotes	Pygo1
T22376	7661-7662	HYPH	denotes	/
T22375	7662-7667	NN	denotes	Pygo2
T22377	7668-7672	JJ	denotes	null
T22378	7673-7680	NNS	denotes	embryos
T22379	7681-7685	RB	denotes	also
T22368	7686-7695	VBD	denotes	displayed
T22380	7696-7707	NN	denotes	exencephaly
T22381	7707-7708	.	denotes	.
T27423	7718-7728	NNS	denotes	Phenotypes
T27424	7729-7731	IN	denotes	of
T27425	7732-7737	NN	denotes	Pygo2
T27427	7738-7742	JJ	denotes	wild
T27428	7742-7743	HYPH	denotes	-
T27426	7743-7747	NN	denotes	type
T27430	7747-7749	,	denotes	,
T27431	7749-7762	JJ	denotes	heterozyogous
T27432	7762-7764	,	denotes	,
T27433	7764-7767	CC	denotes	and
T27434	7768-7772	JJ	denotes	null
T27435	7773-7778	NN	denotes	E18.5
T27429	7779-7786	NNS	denotes	embryos
T27436	7787-7789	IN	denotes	on
T27437	7790-7791	DT	denotes	a
T27439	7792-7797	NN	denotes	Pygo1
T27440	7798-7802	JJ	denotes	null
T27438	7803-7813	NN	denotes	background
T27441	7813-7814	.	denotes	.
T4629	8190-8200	NN	denotes	Expression
T4630	8201-8203	IN	denotes	of
T4631	8204-8209	NN	denotes	Pygo1
T4632	8210-8213	CC	denotes	and
T4633	8214-8219	NN	denotes	Pygo2
T4634	8220-8222	IN	denotes	in
T4635	8223-8226	DT	denotes	the
T4637	8227-8237	VBG	denotes	developing
T4636	8238-8244	NN	denotes	kidney
T4638	8244-8393	sentence	denotes	In situ hybridization has been used previously to characterize expression of Pygo1 [14,16](Yu J, Valerius MT, McMahon AP, contribution to GUDMAP, ).
T4639	8245-8247	FW	denotes	In
T4640	8248-8252	FW	denotes	situ
T4641	8253-8266	NN	denotes	hybridization
T4643	8267-8270	VBZ	denotes	has
T4644	8271-8275	VBN	denotes	been
T4642	8276-8280	VBN	denotes	used
T4645	8281-8291	RB	denotes	previously
T4646	8292-8294	TO	denotes	to
T4647	8295-8307	VB	denotes	characterize
T4648	8308-8318	NN	denotes	expression
T4649	8319-8321	IN	denotes	of
T4650	8322-8327	NN	denotes	Pygo1
T4651	8328-8329	-LRB-	denotes	[
T4653	8329-8331	CD	denotes	14
T4654	8331-8332	,	denotes	,
T4652	8332-8334	CD	denotes	16
T4655	8334-8335	-RRB-	denotes	]
T4656	8335-8336	-LRB-	denotes	(
T4657	8336-8338	NNP	denotes	Yu
T4658	8339-8340	NNP	denotes	J
T4659	8340-8342	,	denotes	,
T4660	8342-8350	NNP	denotes	Valerius
T4661	8351-8353	NNP	denotes	MT
T4662	8353-8355	,	denotes	,
T4663	8355-8362	NNP	denotes	McMahon
T4664	8363-8365	NNP	denotes	AP
T4665	8365-8367	,	denotes	,
T4666	8367-8379	NN	denotes	contribution
T4667	8380-8382	IN	denotes	to
T4668	8383-8389	NN	denotes	GUDMAP
T4669	8389-8391	,	denotes	,
T4670	8391-8392	-RRB-	denotes	)
T4671	8392-8393	.	denotes	.
T4672	8393-8532	sentence	denotes	In this report, we used immunofluorescence to better define the expression patterns of the Pygo1 and Pygo2 genes in the developing kidney.
T4673	8394-8396	IN	denotes	In
T4675	8397-8401	DT	denotes	this
T4676	8402-8408	NN	denotes	report
T4677	8408-8410	,	denotes	,
T4678	8410-8412	PRP	denotes	we
T4674	8413-8417	VBD	denotes	used
T4679	8418-8436	NN	denotes	immunofluorescence
T4680	8437-8439	TO	denotes	to
T4682	8440-8446	RBR	denotes	better
T4681	8447-8453	VB	denotes	define
T4683	8454-8457	DT	denotes	the
T4685	8458-8468	NN	denotes	expression
T4684	8469-8477	NNS	denotes	patterns
T4686	8478-8480	IN	denotes	of
T4687	8481-8484	DT	denotes	the
T4689	8485-8490	NN	denotes	Pygo1
T4690	8491-8494	CC	denotes	and
T4691	8495-8500	NN	denotes	Pygo2
T4688	8501-8506	NNS	denotes	genes
T4692	8507-8509	IN	denotes	in
T4693	8510-8513	DT	denotes	the
T4695	8514-8524	VBG	denotes	developing
T4694	8525-8531	NN	denotes	kidney
T4696	8531-8532	.	denotes	.
T4697	8532-8686	sentence	denotes	Both genes were widely expressed, showing nuclear localization of encoded proteins in the ureteric bud, capping mesenchyme, and stromal cells (Figure 2).
T4698	8533-8537	DT	denotes	Both
T4699	8538-8543	NNS	denotes	genes
T4701	8544-8548	VBD	denotes	were
T4702	8549-8555	RB	denotes	widely
T4700	8556-8565	VBN	denotes	expressed
T4703	8565-8567	,	denotes	,
T4704	8567-8574	VBG	denotes	showing
T4705	8575-8582	JJ	denotes	nuclear
T4706	8583-8595	NN	denotes	localization
T4707	8596-8598	IN	denotes	of
T4708	8599-8606	VBN	denotes	encoded
T4709	8607-8615	NN	denotes	proteins
T4710	8616-8618	IN	denotes	in
T4711	8619-8622	DT	denotes	the
T4713	8623-8631	JJ	denotes	ureteric
T4712	8632-8635	NN	denotes	bud
T4714	8635-8637	,	denotes	,
T4715	8637-8644	NN	denotes	capping
T4716	8645-8655	NN	denotes	mesenchyme
T4717	8655-8657	,	denotes	,
T4718	8657-8660	CC	denotes	and
T4719	8661-8668	JJ	denotes	stromal
T4720	8669-8674	NNS	denotes	cells
T4721	8675-8676	-LRB-	denotes	(
T4722	8676-8682	NN	denotes	Figure
T4723	8683-8684	CD	denotes	2
T4724	8684-8685	-RRB-	denotes	)
T4725	8685-8686	.	denotes	.
T4726	8686-8889	sentence	denotes	Raised expression of Pygo1, and to a lesser degree of Pygo2, was seen in stromal cells, and significant nuclear staining was detected in essentially all cells of the developing kidney for both proteins.
T4727	8687-8693	VBN	denotes	Raised
T4728	8694-8704	NN	denotes	expression
T4730	8705-8707	IN	denotes	of
T4731	8708-8713	NN	denotes	Pygo1
T4732	8713-8715	,	denotes	,
T4733	8715-8718	CC	denotes	and
T4734	8719-8721	IN	denotes	to
T4736	8722-8723	DT	denotes	a
T4738	8724-8730	JJR	denotes	lesser
T4737	8731-8737	NN	denotes	degree
T4735	8738-8740	IN	denotes	of
T4739	8741-8746	NN	denotes	Pygo2
T4740	8746-8748	,	denotes	,
T4741	8748-8751	VBD	denotes	was
T4729	8752-8756	VBN	denotes	seen
T4742	8757-8759	IN	denotes	in
T4743	8760-8767	JJ	denotes	stromal
T4744	8768-8773	NNS	denotes	cells
T4745	8773-8775	,	denotes	,
T4746	8775-8778	CC	denotes	and
T4747	8779-8790	JJ	denotes	significant
T4749	8791-8798	JJ	denotes	nuclear
T4748	8799-8807	NN	denotes	staining
T4751	8808-8811	VBD	denotes	was
T4750	8812-8820	VBN	denotes	detected
T4752	8821-8823	IN	denotes	in
T4753	8824-8835	RB	denotes	essentially
T4755	8836-8839	DT	denotes	all
T4754	8840-8845	NNS	denotes	cells
T4756	8846-8848	IN	denotes	of
T4757	8849-8852	DT	denotes	the
T4759	8853-8863	VBG	denotes	developing
T4758	8864-8870	NN	denotes	kidney
T4760	8871-8874	IN	denotes	for
T4761	8875-8879	DT	denotes	both
T4762	8880-8888	NN	denotes	proteins
T4763	8888-8889	.	denotes	.
T22637	8900-8905	NN	denotes	Pygo1
T22639	8906-8909	CC	denotes	and
T22640	8910-8915	NN	denotes	Pygo2
T22638	8916-8926	NN	denotes	expression
T22641	8927-8929	IN	denotes	in
T22642	8930-8933	DT	denotes	the
T22644	8934-8939	NN	denotes	E18.5
T22645	8940-8950	VBG	denotes	developing
T22643	8951-8957	NN	denotes	kidney
T22646	8957-8958	.	denotes	.
T22647	8958-9086	sentence	denotes	Immunofluorescence was used to determine expression patterns of the Pygo1 and Pygo2 proteins in the cortex of the E18.5 kidney.
T22648	8959-8977	NN	denotes	Immunofluorescence
T22650	8978-8981	VBD	denotes	was
T22649	8982-8986	VBN	denotes	used
T22651	8987-8989	TO	denotes	to
T22652	8990-8999	VB	denotes	determine
T22653	9000-9010	NN	denotes	expression
T22654	9011-9019	NNS	denotes	patterns
T22655	9020-9022	IN	denotes	of
T22656	9023-9026	DT	denotes	the
T22658	9027-9032	NN	denotes	Pygo1
T22659	9033-9036	CC	denotes	and
T22660	9037-9042	NN	denotes	Pygo2
T22657	9043-9051	NN	denotes	proteins
T22661	9052-9054	IN	denotes	in
T22662	9055-9058	DT	denotes	the
T22663	9059-9065	NN	denotes	cortex
T22664	9066-9068	IN	denotes	of
T22665	9069-9072	DT	denotes	the
T22667	9073-9078	NN	denotes	E18.5
T22666	9079-9085	NN	denotes	kidney
T22668	9085-9086	.	denotes	.
T22669	9086-9157	sentence	denotes	Both Pygo1 and Pygo2 (red) were localized in the nucleus, as expected.
T22670	9087-9091	CC	denotes	Both
T22671	9092-9097	NN	denotes	Pygo1
T22673	9098-9101	CC	denotes	and
T22674	9102-9107	NN	denotes	Pygo2
T22675	9108-9109	-LRB-	denotes	(
T22676	9109-9112	JJ	denotes	red
T22677	9112-9113	-RRB-	denotes	)
T22678	9114-9118	VBD	denotes	were
T22672	9119-9128	VBN	denotes	localized
T22679	9129-9131	IN	denotes	in
T22680	9132-9135	DT	denotes	the
T22681	9136-9143	NN	denotes	nucleus
T22682	9143-9145	,	denotes	,
T22683	9145-9147	IN	denotes	as
T22684	9148-9156	VBN	denotes	expected
T22685	9156-9157	.	denotes	.
T22686	9157-9330	sentence	denotes	Both genes showed widespread expression, with signal detected in all components of the developing kidney, but with elevated levels in the stromal cell compartment (arrows).
T22687	9158-9162	DT	denotes	Both
T22688	9163-9168	NNS	denotes	genes
T22689	9169-9175	VBD	denotes	showed
T22690	9176-9186	JJ	denotes	widespread
T22691	9187-9197	NN	denotes	expression
T22692	9197-9199	,	denotes	,
T22693	9199-9203	IN	denotes	with
T22694	9204-9210	NN	denotes	signal
T22695	9211-9219	VBN	denotes	detected
T22696	9220-9222	IN	denotes	in
T22697	9223-9226	DT	denotes	all
T22698	9227-9237	NNS	denotes	components
T22699	9238-9240	IN	denotes	of
T22700	9241-9244	DT	denotes	the
T22702	9245-9255	VBG	denotes	developing
T22701	9256-9262	NN	denotes	kidney
T22703	9262-9264	,	denotes	,
T22704	9264-9267	CC	denotes	but
T22705	9268-9272	IN	denotes	with
T22706	9273-9281	VBN	denotes	elevated
T22707	9282-9288	NNS	denotes	levels
T22708	9289-9291	IN	denotes	in
T22709	9292-9295	DT	denotes	the
T22711	9296-9303	JJ	denotes	stromal
T22712	9304-9308	NN	denotes	cell
T22710	9309-9320	NN	denotes	compartment
T22713	9321-9322	-LRB-	denotes	(
T22714	9322-9328	NNS	denotes	arrows
T22715	9328-9329	-RRB-	denotes	)
T22716	9329-9330	.	denotes	.
T22717	9330-9435	sentence	denotes	Epithelial cells, primarily ureteric bud in these sections, were labeled blue using E-cadherin antibody.
T22718	9331-9341	JJ	denotes	Epithelial
T22719	9342-9347	NNS	denotes	cells
T22721	9347-9349	,	denotes	,
T22722	9349-9358	RB	denotes	primarily
T22724	9359-9367	JJ	denotes	ureteric
T22723	9368-9371	NN	denotes	bud
T22725	9372-9374	IN	denotes	in
T22726	9375-9380	DT	denotes	these
T22727	9381-9389	NNS	denotes	sections
T22728	9389-9391	,	denotes	,
T22729	9391-9395	VBD	denotes	were
T22720	9396-9403	VBN	denotes	labeled
T22730	9404-9408	NN	denotes	blue
T22731	9409-9414	VBG	denotes	using
T22732	9415-9416	NN	denotes	E
T22734	9416-9417	HYPH	denotes	-
T22733	9417-9425	NN	denotes	cadherin
T22735	9426-9434	NN	denotes	antibody
T22736	9434-9435	.	denotes	.
T22737	9435-9465	sentence	denotes	Original magnification × 200.
T22738	9436-9444	JJ	denotes	Original
T22739	9445-9458	NN	denotes	magnification
T22741	9459-9460	SYM	denotes	×
T22740	9461-9464	CD	denotes	200
T22742	9464-9465	.	denotes	.
T5653	9467-9475	JJ	denotes	Confocal
T5654	9476-9484	NN	denotes	analysis
T5655	9485-9487	IN	denotes	of
T5656	9488-9493	NN	denotes	Pygo1
T5658	9493-9494	HYPH	denotes	/
T5657	9494-9499	NN	denotes	Pygo2
T5659	9500-9506	NN	denotes	mutant
T5660	9507-9514	NNS	denotes	kidneys
T5661	9514-9664	sentence	denotes	Histological examination of Pygo2 null and Pygo1/Pygo2 double-null mutant kidneys did not reveal any abnormalities in nephrogenesis (data not shown).
T5662	9515-9527	JJ	denotes	Histological
T5663	9528-9539	NN	denotes	examination
T5665	9540-9542	IN	denotes	of
T5666	9543-9548	NN	denotes	Pygo2
T5667	9549-9553	JJ	denotes	null
T5669	9554-9557	CC	denotes	and
T5670	9558-9563	NN	denotes	Pygo1
T5672	9563-9564	HYPH	denotes	/
T5671	9564-9569	NN	denotes	Pygo2
T5674	9570-9576	JJ	denotes	double
T5675	9576-9577	HYPH	denotes	-
T5673	9577-9581	JJ	denotes	null
T5676	9582-9588	NN	denotes	mutant
T5668	9589-9596	NNS	denotes	kidneys
T5677	9597-9600	VBD	denotes	did
T5678	9601-9604	RB	denotes	not
T5664	9605-9611	VB	denotes	reveal
T5679	9612-9615	DT	denotes	any
T5680	9616-9629	NNS	denotes	abnormalities
T5681	9630-9632	IN	denotes	in
T5682	9633-9646	NN	denotes	nephrogenesis
T5683	9647-9648	-LRB-	denotes	(
T5685	9648-9652	NNS	denotes	data
T5686	9653-9656	RB	denotes	not
T5684	9657-9662	VBN	denotes	shown
T5687	9662-9663	-RRB-	denotes	)
T5688	9663-9664	.	denotes	.
T5689	9664-9801	sentence	denotes	Confocal analysis was therefore performed to characterize renal development in E18.5 Pygo1/Pygo2 null kidneys more precisely (Figure 3).
T5690	9665-9673	JJ	denotes	Confocal
T5691	9674-9682	NN	denotes	analysis
T5693	9683-9686	VBD	denotes	was
T5694	9687-9696	RB	denotes	therefore
T5692	9697-9706	VBN	denotes	performed
T5695	9707-9709	TO	denotes	to
T5696	9710-9722	VB	denotes	characterize
T5697	9723-9728	JJ	denotes	renal
T5698	9729-9740	NN	denotes	development
T5699	9741-9743	IN	denotes	in
T5700	9744-9749	NN	denotes	E18.5
T5702	9750-9755	NN	denotes	Pygo1
T5704	9755-9756	HYPH	denotes	/
T5703	9756-9761	NN	denotes	Pygo2
T5705	9762-9766	JJ	denotes	null
T5701	9767-9774	NNS	denotes	kidneys
T5706	9775-9779	RBR	denotes	more
T5707	9780-9789	RB	denotes	precisely
T5708	9790-9791	-LRB-	denotes	(
T5709	9791-9797	NN	denotes	Figure
T5710	9798-9799	CD	denotes	3
T5711	9799-9800	-RRB-	denotes	)
T5712	9800-9801	.	denotes	.
T5713	9801-9920	sentence	denotes	Wt1 (red) and Cited1 (red) antibodies both stain the capping metanephric mesenchyme around the ureteric bud tips [17].
T5714	9802-9805	NN	denotes	Wt1
T5716	9806-9807	-LRB-	denotes	(
T5717	9807-9810	NN	denotes	red
T5718	9810-9811	-RRB-	denotes	)
T5719	9812-9815	CC	denotes	and
T5720	9816-9822	NN	denotes	Cited1
T5721	9823-9824	-LRB-	denotes	(
T5722	9824-9827	NN	denotes	red
T5723	9827-9828	-RRB-	denotes	)
T5715	9829-9839	NNS	denotes	antibodies
T5725	9840-9844	DT	denotes	both
T5724	9845-9850	VBP	denotes	stain
T5726	9851-9854	DT	denotes	the
T5728	9855-9862	VBG	denotes	capping
T5729	9863-9874	JJ	denotes	metanephric
T5727	9875-9885	NN	denotes	mesenchyme
T5730	9886-9892	IN	denotes	around
T5731	9893-9896	DT	denotes	the
T5733	9897-9905	JJ	denotes	ureteric
T5734	9906-9909	NN	denotes	bud
T5732	9910-9914	NNS	denotes	tips
T5735	9915-9916	-LRB-	denotes	[
T5736	9916-9918	CD	denotes	17
T5737	9918-9919	-RRB-	denotes	]
T5738	9919-9920	.	denotes	.
T5739	9920-9983	sentence	denotes	WT1 antibody also stains renal vesicles and glomerular anlage.
T5740	9921-9924	NN	denotes	WT1
T5741	9925-9933	NN	denotes	antibody
T5743	9934-9938	RB	denotes	also
T5742	9939-9945	VBZ	denotes	stains
T5744	9946-9951	JJ	denotes	renal
T5745	9952-9960	NNS	denotes	vesicles
T5746	9961-9964	CC	denotes	and
T5747	9965-9975	JJ	denotes	glomerular
T5748	9976-9982	NN	denotes	anlage
T5749	9982-9983	.	denotes	.
T5750	9983-10170	sentence	denotes	Antibodies to Cdh1, also known as E-cadherin (blue), were used to identify epithelial structures, including the branching ureteric bud and nascent nephrons of the developing kidney [18].
T5751	9984-9994	NNS	denotes	Antibodies
T5753	9995-9997	IN	denotes	to
T5754	9998-10002	NN	denotes	Cdh1
T5755	10002-10004	,	denotes	,
T5756	10004-10008	RB	denotes	also
T5757	10009-10014	VBN	denotes	known
T5758	10015-10017	IN	denotes	as
T5759	10018-10019	NN	denotes	E
T5761	10019-10020	HYPH	denotes	-
T5760	10020-10028	NN	denotes	cadherin
T5762	10029-10030	-LRB-	denotes	(
T5763	10030-10034	NN	denotes	blue
T5764	10034-10035	-RRB-	denotes	)
T5765	10035-10037	,	denotes	,
T5766	10037-10041	VBD	denotes	were
T5752	10042-10046	VBN	denotes	used
T5767	10047-10049	TO	denotes	to
T5768	10050-10058	VB	denotes	identify
T5769	10059-10069	JJ	denotes	epithelial
T5770	10070-10080	NNS	denotes	structures
T5771	10080-10082	,	denotes	,
T5772	10082-10091	VBG	denotes	including
T5773	10092-10095	DT	denotes	the
T5775	10096-10105	VBG	denotes	branching
T5776	10106-10114	JJ	denotes	ureteric
T5774	10115-10118	NN	denotes	bud
T5777	10119-10122	CC	denotes	and
T5778	10123-10130	JJ	denotes	nascent
T5779	10131-10139	NNS	denotes	nephrons
T5780	10140-10142	IN	denotes	of
T5781	10143-10146	DT	denotes	the
T5783	10147-10157	VBG	denotes	developing
T5782	10158-10164	NN	denotes	kidney
T5784	10165-10166	-LRB-	denotes	[
T5785	10166-10168	CD	denotes	18
T5786	10168-10169	-RRB-	denotes	]
T5787	10169-10170	.	denotes	.
T5788	10170-10274	sentence	denotes	Dolichos biflorus (DBA) lectin [16] was used to selectively stain ureteric bud-derived structures [19].
T5789	10171-10179	NN	denotes	Dolichos
T5790	10180-10188	NN	denotes	biflorus
T5792	10189-10190	-LRB-	denotes	(
T5793	10190-10193	NN	denotes	DBA
T5794	10193-10194	-RRB-	denotes	)
T5791	10195-10201	NN	denotes	lectin
T5796	10202-10203	-LRB-	denotes	[
T5797	10203-10205	CD	denotes	16
T5798	10205-10206	-RRB-	denotes	]
T5799	10207-10210	VBD	denotes	was
T5795	10211-10215	VBN	denotes	used
T5800	10216-10218	TO	denotes	to
T5802	10219-10230	RB	denotes	selectively
T5801	10231-10236	VB	denotes	stain
T5803	10237-10245	JJ	denotes	ureteric
T5804	10246-10249	NN	denotes	bud
T5806	10249-10250	HYPH	denotes	-
T5805	10250-10257	VBN	denotes	derived
T5807	10258-10268	NNS	denotes	structures
T5808	10269-10270	-LRB-	denotes	[
T5809	10270-10272	CD	denotes	19
T5810	10272-10273	-RRB-	denotes	]
T5811	10273-10274	.	denotes	.
T5812	10274-10275	sentence	denotes
T23117	10285-10293	JJ	denotes	Confocal
T23118	10294-10302	NN	denotes	analysis
T23119	10303-10305	IN	denotes	of
T23120	10306-10311	NN	denotes	Pygo1
T23122	10311-10312	HYPH	denotes	/
T23121	10312-10317	NN	denotes	Pygo2
T23123	10318-10324	NN	denotes	mutant
T23125	10325-10330	NN	denotes	E18.5
T23124	10331-10338	NNS	denotes	kidneys
T23126	10338-10339	.	denotes	.
T23127	10339-10657	sentence	denotes	(B, D) Pygo1-/-/Pygo2-/- mutant E18.5 kidneys and (A, C) kidneys of normal littermates were stained using antibodies to (C, D) Cited1 or (A, B) Wt1, both expressed in the condensing metanephric mesenchyme, and colored as red, Cdh1, a general marker of epithelia (blue), and DBA lectin staining the ureteric tree [16].
T23128	10340-10341	-LRB-	denotes	(
T23129	10341-10342	LS	denotes	B
T23131	10342-10344	,	denotes	,
T23132	10344-10345	LS	denotes	D
T23133	10345-10346	-RRB-	denotes	)
T23134	10347-10352	NN	denotes	Pygo1
T23136	10352-10353	SYM	denotes	-
T23137	10353-10354	HYPH	denotes	/
T23138	10354-10355	SYM	denotes	-
T23139	10355-10356	HYPH	denotes	/
T23140	10356-10361	NN	denotes	Pygo2
T23141	10361-10362	SYM	denotes	-
T23142	10362-10363	HYPH	denotes	/
T23143	10363-10364	SYM	denotes	-
T23135	10365-10371	NN	denotes	mutant
T23145	10372-10377	NN	denotes	E18.5
T23144	10378-10385	NNS	denotes	kidneys
T23146	10386-10389	CC	denotes	and
T23147	10390-10391	-LRB-	denotes	(
T23149	10391-10392	NN	denotes	A
T23150	10392-10394	,	denotes	,
T23148	10394-10395	NN	denotes	C
T23152	10395-10396	-RRB-	denotes	)
T23151	10397-10404	NNS	denotes	kidneys
T23153	10405-10407	IN	denotes	of
T23154	10408-10414	JJ	denotes	normal
T23155	10415-10426	NNS	denotes	littermates
T23156	10427-10431	VBD	denotes	were
T23130	10432-10439	VBN	denotes	stained
T23157	10440-10445	VBG	denotes	using
T23158	10446-10456	NNS	denotes	antibodies
T23159	10457-10459	IN	denotes	to
T23160	10460-10461	-LRB-	denotes	(
T23162	10461-10462	NN	denotes	C
T23163	10462-10464	,	denotes	,
T23161	10464-10465	NN	denotes	D
T23165	10465-10466	-RRB-	denotes	)
T23164	10467-10473	NN	denotes	Cited1
T23166	10474-10476	CC	denotes	or
T23167	10477-10478	-LRB-	denotes	(
T23169	10478-10479	NN	denotes	A
T23170	10479-10481	,	denotes	,
T23168	10481-10482	NN	denotes	B
T23172	10482-10483	-RRB-	denotes	)
T23171	10484-10487	NN	denotes	Wt1
T23173	10487-10489	,	denotes	,
T23174	10489-10493	RB	denotes	both
T23175	10494-10503	VBN	denotes	expressed
T23176	10504-10506	IN	denotes	in
T23177	10507-10510	DT	denotes	the
T23179	10511-10521	VBG	denotes	condensing
T23180	10522-10533	JJ	denotes	metanephric
T23178	10534-10544	NN	denotes	mesenchyme
T23181	10544-10546	,	denotes	,
T23182	10546-10549	CC	denotes	and
T23183	10550-10557	VBN	denotes	colored
T23184	10558-10560	IN	denotes	as
T23185	10561-10564	NN	denotes	red
T23186	10564-10566	,	denotes	,
T23187	10566-10570	NN	denotes	Cdh1
T23188	10570-10572	,	denotes	,
T23189	10572-10573	DT	denotes	a
T23191	10574-10581	JJ	denotes	general
T23190	10582-10588	NN	denotes	marker
T23192	10589-10591	IN	denotes	of
T23193	10592-10601	NNS	denotes	epithelia
T23194	10602-10603	-LRB-	denotes	(
T23195	10603-10607	NN	denotes	blue
T23196	10607-10608	-RRB-	denotes	)
T23197	10608-10610	,	denotes	,
T23198	10610-10613	CC	denotes	and
T23199	10614-10617	NN	denotes	DBA
T23200	10618-10624	NN	denotes	lectin
T23201	10625-10633	VBG	denotes	staining
T23202	10634-10637	DT	denotes	the
T23204	10638-10646	JJ	denotes	ureteric
T23203	10647-10651	NN	denotes	tree
T23205	10652-10653	-LRB-	denotes	[
T23206	10653-10655	CD	denotes	16
T23207	10655-10656	-RRB-	denotes	]
T23208	10656-10657	.	denotes	.
T23209	10657-10716	sentence	denotes	Confocal Z-sections were obtained every 5 μm for 75–80 μm.
T23210	10658-10666	JJ	denotes	Confocal
T23212	10667-10668	NN	denotes	Z
T23213	10668-10669	HYPH	denotes	-
T23211	10669-10677	NNS	denotes	sections
T23215	10678-10682	VBD	denotes	were
T23214	10683-10691	VBN	denotes	obtained
T23216	10692-10697	DT	denotes	every
T23218	10698-10699	CD	denotes	5
T23217	10700-10702	NN	denotes	μm
T23219	10703-10706	IN	denotes	for
T23220	10707-10709	CD	denotes	75
T23222	10709-10710	SYM	denotes	–
T23221	10710-10712	CD	denotes	80
T23223	10713-10715	NN	denotes	μm
T23224	10715-10716	.	denotes	.
T23225	10716-10853	sentence	denotes	A reduced number of ureteric tips per area was observed in (B, D) the Pygo1/Pygo2 null kidneys compared with (A, C) control littermates.
T23226	10717-10718	DT	denotes	A
T23228	10719-10726	VBN	denotes	reduced
T23227	10727-10733	NN	denotes	number
T23230	10734-10736	IN	denotes	of
T23231	10737-10745	JJ	denotes	ureteric
T23232	10746-10750	NNS	denotes	tips
T23233	10751-10754	IN	denotes	per
T23234	10755-10759	NN	denotes	area
T23235	10760-10763	VBD	denotes	was
T23229	10764-10772	VBN	denotes	observed
T23236	10773-10775	IN	denotes	in
T23237	10776-10777	-LRB-	denotes	(
T23239	10777-10778	NN	denotes	B
T23240	10778-10780	,	denotes	,
T23238	10780-10781	NN	denotes	D
T23242	10781-10782	-RRB-	denotes	)
T23243	10783-10786	DT	denotes	the
T23244	10787-10792	NN	denotes	Pygo1
T23246	10792-10793	HYPH	denotes	/
T23245	10793-10798	NN	denotes	Pygo2
T23247	10799-10803	JJ	denotes	null
T23241	10804-10811	NNS	denotes	kidneys
T23248	10812-10820	VBN	denotes	compared
T23249	10821-10825	IN	denotes	with
T23250	10826-10827	-LRB-	denotes	(
T23252	10827-10828	NN	denotes	A
T23253	10828-10830	,	denotes	,
T23251	10830-10831	NN	denotes	C
T23255	10831-10832	-RRB-	denotes	)
T23256	10833-10840	NN	denotes	control
T23254	10841-10852	NNS	denotes	littermates
T23257	10852-10853	.	denotes	.
T23258	10853-10956	sentence	denotes	In addition, ureteric bud tips were often more dilated in Pygo1/2 null mutants compared with controls.
T23259	10854-10856	IN	denotes	In
T23261	10857-10865	NN	denotes	addition
T23262	10865-10867	,	denotes	,
T23263	10867-10875	JJ	denotes	ureteric
T23264	10876-10879	NN	denotes	bud
T23265	10880-10884	NNS	denotes	tips
T23260	10885-10889	VBD	denotes	were
T23266	10890-10895	RB	denotes	often
T23267	10896-10900	RBR	denotes	more
T23268	10901-10908	JJ	denotes	dilated
T23269	10909-10911	IN	denotes	in
T23270	10912-10917	NN	denotes	Pygo1
T23272	10917-10918	HYPH	denotes	/
T23273	10918-10919	CD	denotes	2
T23271	10920-10924	JJ	denotes	null
T23274	10925-10932	NNS	denotes	mutants
T23275	10933-10941	VBN	denotes	compared
T23276	10942-10946	IN	denotes	with
T23277	10947-10955	NNS	denotes	controls
T23278	10955-10956	.	denotes	.
T23279	10956-11058	sentence	denotes	The condensed mesenchyme surrounding the ureteric bud was also significantly expanded in the mutants.
T23280	10957-10960	DT	denotes	The
T23282	10961-10970	VBN	denotes	condensed
T23281	10971-10981	NN	denotes	mesenchyme
T23284	10982-10993	VBG	denotes	surrounding
T23285	10994-10997	DT	denotes	the
T23287	10998-11006	JJ	denotes	ureteric
T23286	11007-11010	NN	denotes	bud
T23288	11011-11014	VBD	denotes	was
T23289	11015-11019	RB	denotes	also
T23290	11020-11033	RB	denotes	significantly
T23283	11034-11042	VBN	denotes	expanded
T23291	11043-11045	IN	denotes	in
T23292	11046-11049	DT	denotes	the
T23293	11050-11057	NNS	denotes	mutants
T23294	11057-11058	.	denotes	.
T23295	11058-11088	sentence	denotes	Original magnification × 200.
T23296	11059-11067	JJ	denotes	Original
T23297	11068-11081	NN	denotes	magnification
T23299	11082-11083	SYM	denotes	×
T23298	11084-11087	CD	denotes	200
T23300	11087-11088	.	denotes	.
T5814	11089-11092	NN	denotes	Wt1
T5813	11089-11103	sentence	denotes	Wt1 and Cited1
T5815	11092-11093	,	denotes
T5816	11093-11095	JJ	denotes	an
T5817	11095-11096	CC	denotes	d
T5818	11097-11098	NN	denotes	C
T5819	11098-11101	NNS	denotes	ite
T5820	11101-11102	CC	denotes	d
T5821	11102-11103	CD	denotes	1
T5822	11103-11104	sentence	denotes
T5824	11104-11105	-LRB-	denotes	(
T5823	11104-11111	sentence	denotes	(red) s
T5825	11105-11108	VBN	denotes	red
T5827	11108-11109	-RRB-	denotes	)
T5826	11110-11111	NNS	denotes	s
T5829	11111-11112	NN	denotes	t
T5828	11111-11117	sentence	denotes	tainin
T5830	11112-11113	JJ	denotes	a
T5831	11113-11115	IN	denotes	in
T5832	11115-11116	IN	denotes	i
T5833	11116-11117	NNS	denotes	n
T5835	11117-11118	VBG	denotes	g
T5834	11117-11121	sentence	denotes	g re
T5836	11119-11121	JJ	denotes	re
T5838	11121-11127	VBD	denotes	vealed
T5837	11121-11261	sentence	denotes	vealed an increase of approximately 30% in the thickness of the capping mesenchyme surrounding the mutant ureteric buds (Figure 3, Table 2).
T5839	11128-11130	DT	denotes	an
T5840	11131-11139	NN	denotes	increase
T5841	11140-11142	IN	denotes	of
T5842	11143-11156	RB	denotes	approximately
T5843	11157-11159	CD	denotes	30
T5844	11159-11160	NN	denotes	%
T5845	11161-11163	IN	denotes	in
T5846	11164-11167	DT	denotes	the
T5847	11168-11177	NN	denotes	thickness
T5848	11178-11180	IN	denotes	of
T5849	11181-11184	DT	denotes	the
T5851	11185-11192	VBG	denotes	capping
T5850	11193-11203	NN	denotes	mesenchyme
T5852	11204-11215	VBG	denotes	surrounding
T5853	11216-11219	DT	denotes	the
T5855	11220-11226	NN	denotes	mutant
T5856	11227-11235	JJ	denotes	ureteric
T5854	11236-11240	NNS	denotes	buds
T5857	11241-11242	-LRB-	denotes	(
T5859	11242-11248	NN	denotes	Figure
T5860	11249-11250	CD	denotes	3
T5861	11250-11252	,	denotes	,
T5858	11252-11257	NN	denotes	Table
T5862	11258-11259	CD	denotes	2
T5863	11259-11260	-RRB-	denotes	)
T5864	11260-11261	.	denotes	.
T5865	11261-11352	sentence	denotes	Nevertheless, the mutant metanephric mesenchyme underwent relatively normal nephrogenesis.
T5866	11262-11274	RB	denotes	Nevertheless
T5868	11274-11276	,	denotes	,
T5869	11276-11279	DT	denotes	the
T5871	11280-11286	NN	denotes	mutant
T5872	11287-11298	JJ	denotes	metanephric
T5870	11299-11309	NN	denotes	mesenchyme
T5867	11310-11319	VBD	denotes	underwent
T5873	11320-11330	RB	denotes	relatively
T5874	11331-11337	JJ	denotes	normal
T5875	11338-11351	NN	denotes	nephrogenesis
T5876	11351-11352	.	denotes	.
T5877	11352-11496	sentence	denotes	Cdh1-staining nephrons (blue) were identified connecting to the ureteric bud tips [16] and extending into the medulla of the kidney, as normal.
T5878	11353-11357	NN	denotes	Cdh1
T5880	11357-11358	HYPH	denotes	-
T5879	11358-11366	VBG	denotes	staining
T5881	11367-11375	NNS	denotes	nephrons
T5883	11376-11377	-LRB-	denotes	(
T5884	11377-11381	NN	denotes	blue
T5885	11381-11382	-RRB-	denotes	)
T5886	11383-11387	VBD	denotes	were
T5882	11388-11398	VBN	denotes	identified
T5887	11399-11409	VBG	denotes	connecting
T5888	11410-11412	IN	denotes	to
T5889	11413-11416	DT	denotes	the
T5891	11417-11425	JJ	denotes	ureteric
T5892	11426-11429	NN	denotes	bud
T5890	11430-11434	NNS	denotes	tips
T5893	11435-11436	-LRB-	denotes	[
T5894	11436-11438	CD	denotes	16
T5895	11438-11439	-RRB-	denotes	]
T5896	11440-11443	CC	denotes	and
T5897	11444-11453	VBG	denotes	extending
T5898	11454-11458	IN	denotes	into
T5899	11459-11462	DT	denotes	the
T5900	11463-11470	NN	denotes	medulla
T5901	11471-11473	IN	denotes	of
T5902	11474-11477	DT	denotes	the
T5903	11478-11484	NN	denotes	kidney
T5904	11484-11486	,	denotes	,
T5905	11486-11488	IN	denotes	as
T5906	11489-11495	JJ	denotes	normal
T5907	11495-11496	.	denotes	.
T5908	11496-11622	sentence	denotes	Intermediate structures of nephrogenesis, including renal vesicles, comma-shaped bodies, and S-shaped bodies appeared normal.
T5909	11497-11509	JJ	denotes	Intermediate
T5910	11510-11520	NNS	denotes	structures
T5912	11521-11523	IN	denotes	of
T5913	11524-11537	NN	denotes	nephrogenesis
T5914	11537-11539	,	denotes	,
T5915	11539-11548	VBG	denotes	including
T5916	11549-11554	JJ	denotes	renal
T5917	11555-11563	NNS	denotes	vesicles
T5918	11563-11565	,	denotes	,
T5919	11565-11570	NN	denotes	comma
T5921	11570-11571	HYPH	denotes	-
T5920	11571-11577	VBN	denotes	shaped
T5922	11578-11584	NNS	denotes	bodies
T5923	11584-11586	,	denotes	,
T5924	11586-11589	CC	denotes	and
T5925	11590-11591	NN	denotes	S
T5927	11591-11592	HYPH	denotes	-
T5926	11592-11598	VBN	denotes	shaped
T5928	11599-11605	NNS	denotes	bodies
T5911	11606-11614	VBD	denotes	appeared
T5929	11615-11621	JJ	denotes	normal
T5930	11621-11622	.	denotes	.
T5931	11622-11623	sentence	denotes
T5933	11632-11640	JJ	denotes	Confocal
T27487	11632-11640	JJ	denotes	Confocal
T5932	11632-12084	sentence	denotes	Confocal analysis of Pygo2 wild-type, heterozygous, and null E18.5 kidneys on the Pygo1 null background. Genotype Ureteric tip Condensing mesenchyme Ureteric tips/mm2 SD n Mesenchyme condensate size (μm) SD n Pygo2 +/+ 196 8.36 3 16.2 1.09 3 Pygo2 +/- 191 11.6 7 15.6 1.18 5 Pygo2 -/- 147* 12.2 7 20.9 * 1.12 5 *Significant change (P < 0.01) between the three groups. SD, Standard deviation; n, number Confocal analysis also
T5934	11641-11649	NN	denotes	analysis
T27488	11641-11649	NN	denotes	analysis
T27489	11650-11652	IN	denotes	of
T27490	11653-11658	NN	denotes	Pygo2
T27492	11659-11663	JJ	denotes	wild
T27493	11663-11664	HYPH	denotes	-
T27491	11664-11668	NN	denotes	type
T27495	11668-11670	,	denotes	,
T27496	11670-11682	JJ	denotes	heterozygous
T27497	11682-11684	,	denotes	,
T27498	11684-11687	CC	denotes	and
T27499	11688-11692	JJ	denotes	null
T27500	11693-11698	NN	denotes	E18.5
T27494	11699-11706	NNS	denotes	kidneys
T27501	11707-11709	IN	denotes	on
T27502	11710-11713	DT	denotes	the
T27504	11714-11719	NN	denotes	Pygo1
T27505	11720-11724	JJ	denotes	null
T27503	11725-11735	NN	denotes	background
T27506	11735-11736	.	denotes	.
T5936	12079-12080	CC	denotes	a
T5937	12080-12081	JJ	denotes	l
T5935	12081-12082	NNS	denotes	s
T5938	12082-12083	IN	denotes	o
T5940	12084-12085	NNS	denotes	s
T5939	12084-12085	sentence	denotes	s
T5942	12085-12090	VBD	denotes	howed
T5941	12085-12265	sentence	denotes	howed that the ureteric bud tips [16] of Pygo1/Pygo2 null kidneys were dilated and misshaped compared with those of littermates with at least one wild-type Pygo2 allele (Figure 3).
T5943	12091-12095	IN	denotes	that
T5945	12096-12099	DT	denotes	the
T5947	12100-12108	JJ	denotes	ureteric
T5948	12109-12112	NN	denotes	bud
T5946	12113-12117	NNS	denotes	tips
T5949	12118-12119	-LRB-	denotes	[
T5950	12119-12121	CD	denotes	16
T5951	12121-12122	-RRB-	denotes	]
T5952	12123-12125	IN	denotes	of
T5953	12126-12131	NN	denotes	Pygo1
T5955	12131-12132	HYPH	denotes	/
T5954	12132-12137	NN	denotes	Pygo2
T5956	12138-12142	JJ	denotes	null
T5957	12143-12150	NNS	denotes	kidneys
T5944	12151-12155	VBD	denotes	were
T5958	12156-12163	JJ	denotes	dilated
T5959	12164-12167	CC	denotes	and
T5960	12168-12177	JJ	denotes	misshaped
T5961	12178-12186	VBN	denotes	compared
T5962	12187-12191	IN	denotes	with
T5963	12192-12197	DT	denotes	those
T5964	12198-12200	IN	denotes	of
T5965	12201-12212	NNS	denotes	littermates
T5966	12213-12217	IN	denotes	with
T5967	12218-12220	RB	denotes	at
T5969	12221-12226	RBS	denotes	least
T5968	12227-12230	CD	denotes	one
T5971	12231-12235	JJ	denotes	wild
T5973	12235-12236	HYPH	denotes	-
T5972	12236-12240	NN	denotes	type
T5974	12241-12246	NN	denotes	Pygo2
T5970	12247-12253	NN	denotes	allele
T5975	12254-12255	-LRB-	denotes	(
T5976	12255-12261	NN	denotes	Figure
T5977	12262-12263	CD	denotes	3
T5978	12263-12264	-RRB-	denotes	)
T5979	12264-12265	.	denotes	.
T5980	12265-12491	sentence	denotes	In addition, the Pygo1/Pygo2 double-homozygous mutant kidneys also had a decrease of approximately 25% in the number of ureteric bud tips per area compared with littermates with at least one wild-type copy of Pygo2 (Table 2).
T5981	12266-12268	IN	denotes	In
T5983	12269-12277	NN	denotes	addition
T5984	12277-12279	,	denotes	,
T5985	12279-12282	DT	denotes	the
T5987	12283-12288	NN	denotes	Pygo1
T5989	12288-12289	HYPH	denotes	/
T5988	12289-12294	NN	denotes	Pygo2
T5991	12295-12301	JJ	denotes	double
T5992	12301-12302	HYPH	denotes	-
T5990	12302-12312	JJ	denotes	homozygous
T5993	12313-12319	NN	denotes	mutant
T5986	12320-12327	NNS	denotes	kidneys
T5994	12328-12332	RB	denotes	also
T5982	12333-12336	VBD	denotes	had
T5995	12337-12338	DT	denotes	a
T5996	12339-12347	NN	denotes	decrease
T5997	12348-12350	IN	denotes	of
T5998	12351-12364	RB	denotes	approximately
T5999	12365-12367	CD	denotes	25
T6000	12367-12368	NN	denotes	%
T6001	12369-12371	IN	denotes	in
T6002	12372-12375	DT	denotes	the
T6003	12376-12382	NN	denotes	number
T6004	12383-12385	IN	denotes	of
T6005	12386-12394	JJ	denotes	ureteric
T6006	12395-12398	NN	denotes	bud
T6007	12399-12403	NNS	denotes	tips
T6008	12404-12407	IN	denotes	per
T6009	12408-12412	NN	denotes	area
T6010	12413-12421	VBN	denotes	compared
T6011	12422-12426	IN	denotes	with
T6012	12427-12438	NNS	denotes	littermates
T6013	12439-12443	IN	denotes	with
T6014	12444-12446	RB	denotes	at
T6016	12447-12452	RBS	denotes	least
T6015	12453-12456	CD	denotes	one
T6018	12457-12461	JJ	denotes	wild
T6020	12461-12462	HYPH	denotes	-
T6019	12462-12466	NN	denotes	type
T6017	12467-12471	NN	denotes	copy
T6021	12472-12474	IN	denotes	of
T6022	12475-12480	NN	denotes	Pygo2
T6023	12481-12482	-LRB-	denotes	(
T6024	12482-12487	NN	denotes	Table
T6025	12488-12489	CD	denotes	2
T6026	12489-12490	-RRB-	denotes	)
T6027	12490-12491	.	denotes	.
T6028	12491-12604	sentence	denotes	No significant difference in ureteric bud-tip density was seen between Pygo2+/- and Pygo2+/+ embryos (P = 0.33).
T6029	12492-12494	DT	denotes	No
T6031	12495-12506	JJ	denotes	significant
T6030	12507-12517	NN	denotes	difference
T6033	12518-12520	IN	denotes	in
T6034	12521-12529	JJ	denotes	ureteric
T6035	12530-12533	NN	denotes	bud
T6037	12533-12534	HYPH	denotes	-
T6036	12534-12537	NN	denotes	tip
T6038	12538-12545	NN	denotes	density
T6039	12546-12549	VBD	denotes	was
T6032	12550-12554	VBN	denotes	seen
T6040	12555-12562	IN	denotes	between
T6041	12563-12568	NN	denotes	Pygo2
T6043	12568-12569	SYM	denotes	+
T6044	12569-12570	HYPH	denotes	/
T6045	12570-12571	SYM	denotes	-
T6046	12572-12575	CC	denotes	and
T6047	12576-12581	NN	denotes	Pygo2
T6048	12581-12582	SYM	denotes	+
T6049	12582-12583	HYPH	denotes	/
T6050	12583-12584	SYM	denotes	+
T6042	12585-12592	NNS	denotes	embryos
T6051	12593-12594	-LRB-	denotes	(
T6053	12594-12595	NN	denotes	P
T6054	12596-12597	SYM	denotes	=
T6052	12598-12602	CD	denotes	0.33
T6055	12602-12603	-RRB-	denotes	)
T6056	12603-12604	.	denotes	.
T6317	12606-12608	FW	denotes	In
T6318	12609-12613	FW	denotes	situ
T6319	12614-12627	NN	denotes	hybridization
T6320	12627-12686	sentence	denotes	We examined expression of three Wnt genes in Pygo mutants.
T6321	12628-12630	PRP	denotes	We
T6322	12631-12639	VBD	denotes	examined
T6323	12640-12650	NN	denotes	expression
T6324	12651-12653	IN	denotes	of
T6325	12654-12659	CD	denotes	three
T6327	12660-12663	NN	denotes	Wnt
T6326	12664-12669	NNS	denotes	genes
T6328	12670-12672	IN	denotes	in
T6329	12673-12677	NN	denotes	Pygo
T6330	12678-12685	NNS	denotes	mutants
T6331	12685-12686	.	denotes	.
T6332	12686-12830	sentence	denotes	Wnt7b is expressed in the stalks [20], Wnt11 in the tips [4], and Wnt9b in the stalks and weakly in the tips of the branching ureteric bud [2].
T6333	12687-12692	NN	denotes	Wnt7b
T6335	12693-12695	VBZ	denotes	is
T6334	12696-12705	VBN	denotes	expressed
T6336	12706-12708	IN	denotes	in
T6337	12709-12712	DT	denotes	the
T6338	12713-12719	NNS	denotes	stalks
T6339	12720-12721	-LRB-	denotes	[
T6340	12721-12723	CD	denotes	20
T6341	12723-12724	-RRB-	denotes	]
T6342	12724-12726	,	denotes	,
T6343	12726-12731	NN	denotes	Wnt11
T6344	12732-12734	IN	denotes	in
T6345	12735-12738	DT	denotes	the
T6346	12739-12743	NNS	denotes	tips
T6347	12744-12745	-LRB-	denotes	[
T6348	12745-12746	CD	denotes	4
T6349	12746-12747	-RRB-	denotes	]
T6350	12747-12749	,	denotes	,
T6351	12749-12752	CC	denotes	and
T6352	12753-12758	NN	denotes	Wnt9b
T6353	12759-12761	IN	denotes	in
T6354	12762-12765	DT	denotes	the
T6355	12766-12772	NNS	denotes	stalks
T6356	12773-12776	CC	denotes	and
T6357	12777-12783	RB	denotes	weakly
T6358	12784-12786	IN	denotes	in
T6359	12787-12790	DT	denotes	the
T6360	12791-12795	NNS	denotes	tips
T6361	12796-12798	IN	denotes	of
T6362	12799-12802	DT	denotes	the
T6364	12803-12812	NN	denotes	branching
T6365	12813-12821	JJ	denotes	ureteric
T6363	12822-12825	NN	denotes	bud
T6366	12826-12827	-LRB-	denotes	[
T6367	12827-12828	CD	denotes	2
T6368	12828-12829	-RRB-	denotes	]
T6369	12829-12830	.	denotes	.
T6370	12830-12943	sentence	denotes	All three genes showed similar expression patterns in Pygo2+/+ and Pygo1/Pygo2 double-mutant kidneys (Figure 4).
T6371	12831-12834	DT	denotes	All
T6373	12835-12840	CD	denotes	three
T6372	12841-12846	NNS	denotes	genes
T6374	12847-12853	VBD	denotes	showed
T6375	12854-12861	JJ	denotes	similar
T6377	12862-12872	NN	denotes	expression
T6376	12873-12881	NNS	denotes	patterns
T6378	12882-12884	IN	denotes	in
T6379	12885-12890	NN	denotes	Pygo2
T6381	12890-12891	SYM	denotes	+
T6382	12891-12892	HYPH	denotes	/
T6383	12892-12893	SYM	denotes	+
T6384	12894-12897	CC	denotes	and
T6385	12898-12903	NN	denotes	Pygo1
T6387	12903-12904	HYPH	denotes	/
T6386	12904-12909	NN	denotes	Pygo2
T6389	12910-12916	JJ	denotes	double
T6390	12916-12917	HYPH	denotes	-
T6388	12917-12923	NN	denotes	mutant
T6380	12924-12931	NNS	denotes	kidneys
T6391	12932-12933	-LRB-	denotes	(
T6392	12933-12939	NN	denotes	Figure
T6393	12940-12941	CD	denotes	4
T6394	12941-12942	-RRB-	denotes	)
T6395	12942-12943	.	denotes	.
T6396	12943-13149	sentence	denotes	In addition, these in situ hybridization patterns confirmed the confocal microscopy results, showing a reduced number of tips (Wnt11-positive) per surface area in the Pygo1/Pygo2 double-homozygous mutants.
T6397	12944-12946	IN	denotes	In
T6399	12947-12955	NN	denotes	addition
T6400	12955-12957	,	denotes	,
T6401	12957-12962	DT	denotes	these
T6403	12963-12965	FW	denotes	in
T6404	12966-12970	FW	denotes	situ
T6405	12971-12984	NN	denotes	hybridization
T6402	12985-12993	NNS	denotes	patterns
T6398	12994-13003	VBD	denotes	confirmed
T6406	13004-13007	DT	denotes	the
T6408	13008-13016	JJ	denotes	confocal
T6409	13017-13027	NN	denotes	microscopy
T6407	13028-13035	NNS	denotes	results
T6410	13035-13037	,	denotes	,
T6411	13037-13044	VBG	denotes	showing
T6412	13045-13046	DT	denotes	a
T6414	13047-13054	VBN	denotes	reduced
T6413	13055-13061	NN	denotes	number
T6415	13062-13064	IN	denotes	of
T6416	13065-13069	NNS	denotes	tips
T6417	13070-13071	-LRB-	denotes	(
T6418	13071-13076	NN	denotes	Wnt11
T6420	13076-13077	HYPH	denotes	-
T6419	13077-13085	JJ	denotes	positive
T6421	13085-13086	-RRB-	denotes	)
T6422	13087-13090	IN	denotes	per
T6423	13091-13098	NN	denotes	surface
T6424	13099-13103	NN	denotes	area
T6425	13104-13106	IN	denotes	in
T6426	13107-13110	DT	denotes	the
T6428	13111-13116	NN	denotes	Pygo1
T6430	13116-13117	HYPH	denotes	/
T6429	13117-13122	NN	denotes	Pygo2
T6432	13123-13129	JJ	denotes	double
T6433	13129-13130	HYPH	denotes	-
T6431	13130-13140	JJ	denotes	homozygous
T6427	13141-13148	NNS	denotes	mutants
T6434	13148-13149	.	denotes	.
T23566	13160-13170	NN	denotes	Expression
T23567	13171-13173	IN	denotes	of
T23568	13174-13179	NN	denotes	Wnt7b
T23569	13179-13181	,	denotes	,
T23570	13181-13186	NN	denotes	Wnt9b
T23571	13186-13188	,	denotes	,
T23572	13188-13191	CC	denotes	and
T23573	13192-13197	NN	denotes	Wnt11
T23574	13198-13200	IN	denotes	in
T23575	13201-13206	NN	denotes	E18.5
T23577	13207-13212	NN	denotes	Pygo1
T23579	13212-13213	HYPH	denotes	/
T23578	13213-13218	NN	denotes	Pygo2
T23581	13219-13227	NN	denotes	compound
T23580	13228-13232	JJ	denotes	null
T23576	13233-13240	NNS	denotes	kidneys
T23582	13240-13241	.	denotes	.
T23583	13241-13443	sentence	denotes	Whole-mount in situ of hybridizations of (A-C) E18.5 wild-type kidneys and (D-F) Pygo1-/-/Pygo2-/- mutant kidneys with the ureteric bud derivative markers: (A, D) Wnt7b, (B, E) Wnt9b, and (C, F) Wnt11.
T23584	13242-13247	JJ	denotes	Whole
T23586	13247-13248	HYPH	denotes	-
T23585	13248-13253	NN	denotes	mount
T23587	13254-13256	FW	denotes	in
T23588	13257-13261	FW	denotes	situ
T23589	13262-13264	IN	denotes	of
T23590	13265-13279	NNS	denotes	hybridizations
T23591	13280-13282	IN	denotes	of
T23592	13283-13284	-LRB-	denotes	(
T23593	13284-13285	NN	denotes	A
T23595	13285-13286	SYM	denotes	-
T23596	13286-13287	NN	denotes	C
T23597	13287-13288	-RRB-	denotes	)
T23598	13289-13294	NN	denotes	E18.5
T23599	13295-13299	JJ	denotes	wild
T23601	13299-13300	HYPH	denotes	-
T23600	13300-13304	NN	denotes	type
T23594	13305-13312	NNS	denotes	kidneys
T23602	13313-13316	CC	denotes	and
T23603	13317-13318	-LRB-	denotes	(
T23604	13318-13319	NN	denotes	D
T23606	13319-13320	SYM	denotes	-
T23607	13320-13321	NN	denotes	F
T23608	13321-13322	-RRB-	denotes	)
T23609	13323-13328	NN	denotes	Pygo1
T23611	13328-13329	SYM	denotes	-
T23612	13329-13330	HYPH	denotes	/
T23613	13330-13331	SYM	denotes	-
T23614	13331-13332	HYPH	denotes	/
T23615	13332-13337	NN	denotes	Pygo2
T23616	13337-13338	SYM	denotes	-
T23617	13338-13339	HYPH	denotes	/
T23618	13339-13340	SYM	denotes	-
T23610	13341-13347	NN	denotes	mutant
T23605	13348-13355	NNS	denotes	kidneys
T23619	13356-13360	IN	denotes	with
T23620	13361-13364	DT	denotes	the
T23622	13365-13373	JJ	denotes	ureteric
T23623	13374-13377	NN	denotes	bud
T23624	13378-13388	JJ	denotes	derivative
T23621	13389-13396	NNS	denotes	markers
T23625	13396-13398	:	denotes	:
T23626	13398-13399	-LRB-	denotes	(
T23628	13399-13400	NN	denotes	A
T23629	13400-13402	,	denotes	,
T23627	13402-13403	NN	denotes	D
T23631	13403-13404	-RRB-	denotes	)
T23630	13405-13410	NN	denotes	Wnt7b
T23632	13410-13412	,	denotes	,
T23633	13412-13413	-LRB-	denotes	(
T23635	13413-13414	NN	denotes	B
T23636	13414-13416	,	denotes	,
T23634	13416-13417	NN	denotes	E
T23638	13417-13418	-RRB-	denotes	)
T23637	13419-13424	NN	denotes	Wnt9b
T23639	13424-13426	,	denotes	,
T23640	13426-13429	CC	denotes	and
T23641	13430-13431	-LRB-	denotes	(
T23643	13431-13432	NN	denotes	C
T23644	13432-13434	,	denotes	,
T23642	13434-13435	NN	denotes	F
T23646	13435-13436	-RRB-	denotes	)
T23645	13437-13442	NN	denotes	Wnt11
T23647	13442-13443	.	denotes	.
T23648	13443-13603	sentence	denotes	The mutant kidneys showed normal expression patterns for the ureteric stalk markers Wnt7b and Wnt9b, and reduced density of ureteric tips as measured by Wnt11.
T23649	13444-13447	DT	denotes	The
T23651	13448-13454	NN	denotes	mutant
T23650	13455-13462	NNS	denotes	kidneys
T23652	13463-13469	VBD	denotes	showed
T23653	13470-13476	JJ	denotes	normal
T23655	13477-13487	NN	denotes	expression
T23654	13488-13496	NNS	denotes	patterns
T23656	13497-13500	IN	denotes	for
T23657	13501-13504	DT	denotes	the
T23659	13505-13513	JJ	denotes	ureteric
T23660	13514-13519	NN	denotes	stalk
T23658	13520-13527	NNS	denotes	markers
T23661	13528-13533	NN	denotes	Wnt7b
T23662	13534-13537	CC	denotes	and
T23663	13538-13543	NN	denotes	Wnt9b
T23664	13543-13545	,	denotes	,
T23665	13545-13548	CC	denotes	and
T23666	13549-13556	VBN	denotes	reduced
T23667	13557-13564	NN	denotes	density
T23668	13565-13567	IN	denotes	of
T23669	13568-13576	JJ	denotes	ureteric
T23670	13577-13581	NNS	denotes	tips
T23671	13582-13584	IN	denotes	as
T23672	13585-13593	VBN	denotes	measured
T23673	13594-13596	IN	denotes	by
T23674	13597-13602	NN	denotes	Wnt11
T23675	13602-13603	.	denotes	.
T23676	13603-13632	sentence	denotes	Original magnification × 32.
T23677	13604-13612	JJ	denotes	Original
T23678	13613-13626	NN	denotes	magnification
T23680	13627-13628	SYM	denotes	×
T23679	13629-13631	CD	denotes	32
T23681	13631-13632	.	denotes	.
T9319	13634-13641	VBN	denotes	Reduced
T9321	13642-13651	JJ	denotes	canonical
T9322	13652-13655	NN	denotes	Wnt
T9320	13656-13665	NN	denotes	signaling
T9323	13666-13668	IN	denotes	in
T9324	13669-13674	NN	denotes	Pygo1
T9326	13674-13675	HYPH	denotes	/
T9325	13675-13680	NN	denotes	Pygo2
T9327	13681-13687	NN	denotes	mutant
T9328	13688-13692	NNS	denotes	mice
T9329	13692-13820	sentence	denotes	The BAT-gal transgene reporter of canonical Wnt signaling [21] was used to examine changes in Wnt signaling in the mutant mice.
T9330	13693-13696	DT	denotes	The
T9332	13697-13700	NN	denotes	BAT
T9334	13700-13701	HYPH	denotes	-
T9333	13701-13704	NN	denotes	gal
T9335	13705-13714	NN	denotes	transgene
T9331	13715-13723	NN	denotes	reporter
T9337	13724-13726	IN	denotes	of
T9338	13727-13736	JJ	denotes	canonical
T9340	13737-13740	NN	denotes	Wnt
T9339	13741-13750	NN	denotes	signaling
T9341	13751-13752	-LRB-	denotes	[
T9342	13752-13754	CD	denotes	21
T9343	13754-13755	-RRB-	denotes	]
T9344	13756-13759	VBD	denotes	was
T9336	13760-13764	VBN	denotes	used
T9345	13765-13767	TO	denotes	to
T9346	13768-13775	VB	denotes	examine
T9347	13776-13783	NNS	denotes	changes
T9348	13784-13786	IN	denotes	in
T9349	13787-13790	NN	denotes	Wnt
T9350	13791-13800	NN	denotes	signaling
T9351	13801-13803	IN	denotes	in
T9352	13804-13807	DT	denotes	the
T9354	13808-13814	NN	denotes	mutant
T9353	13815-13819	NNS	denotes	mice
T9355	13819-13820	.	denotes	.
T9356	13820-13933	sentence	denotes	Both Pygo2-/- and Pygo1/Pygo2 double-null E10.5 embryos showed a decrease in canonical Wnt signaling (Figure 5).
T9357	13821-13825	CC	denotes	Both
T9358	13826-13831	NN	denotes	Pygo2
T9360	13831-13832	SYM	denotes	-
T9361	13832-13833	HYPH	denotes	/
T9362	13833-13834	SYM	denotes	-
T9363	13835-13838	CC	denotes	and
T9364	13839-13844	NN	denotes	Pygo1
T9366	13844-13845	HYPH	denotes	/
T9365	13845-13850	NN	denotes	Pygo2
T9367	13851-13857	JJ	denotes	double
T9368	13857-13858	HYPH	denotes	-
T9359	13858-13862	JJ	denotes	null
T9370	13863-13868	NN	denotes	E10.5
T9369	13869-13876	NNS	denotes	embryos
T9371	13877-13883	VBD	denotes	showed
T9372	13884-13885	DT	denotes	a
T9373	13886-13894	NN	denotes	decrease
T9374	13895-13897	IN	denotes	in
T9375	13898-13907	JJ	denotes	canonical
T9377	13908-13911	NN	denotes	Wnt
T9376	13912-13921	NN	denotes	signaling
T9378	13922-13923	-LRB-	denotes	(
T9379	13923-13929	NN	denotes	Figure
T9380	13930-13931	CD	denotes	5
T9381	13931-13932	-RRB-	denotes	)
T9382	13932-13933	.	denotes	.
T9383	13933-14132	sentence	denotes	There was, however, tissue-specific variability in the degree of reduction, with the somites, for example, showing strong reduction, while the mutant telencephalon still showed robust Wnt signaling.
T9384	13934-13939	EX	denotes	There
T9385	13940-13943	VBD	denotes	was
T9386	13943-13945	,	denotes	,
T9387	13945-13952	RB	denotes	however
T9388	13952-13954	,	denotes	,
T9389	13954-13960	NN	denotes	tissue
T9391	13960-13961	HYPH	denotes	-
T9390	13961-13969	JJ	denotes	specific
T9392	13970-13981	NN	denotes	variability
T9393	13982-13984	IN	denotes	in
T9394	13985-13988	DT	denotes	the
T9395	13989-13995	NN	denotes	degree
T9396	13996-13998	IN	denotes	of
T9397	13999-14008	NN	denotes	reduction
T9398	14008-14010	,	denotes	,
T9399	14010-14014	IN	denotes	with
T9400	14015-14018	DT	denotes	the
T9401	14019-14026	NNS	denotes	somites
T9403	14026-14028	,	denotes	,
T9404	14028-14031	IN	denotes	for
T9405	14032-14039	NN	denotes	example
T9406	14039-14041	,	denotes	,
T9402	14041-14048	VBG	denotes	showing
T9407	14049-14055	JJ	denotes	strong
T9408	14056-14065	NN	denotes	reduction
T9409	14065-14067	,	denotes	,
T9410	14067-14072	IN	denotes	while
T9412	14073-14076	DT	denotes	the
T9414	14077-14083	NN	denotes	mutant
T9413	14084-14097	NN	denotes	telencephalon
T9415	14098-14103	RB	denotes	still
T9411	14104-14110	VBD	denotes	showed
T9416	14111-14117	JJ	denotes	robust
T9418	14118-14121	NN	denotes	Wnt
T9417	14122-14131	NN	denotes	signaling
T9419	14131-14132	.	denotes	.
T9420	14132-14282	sentence	denotes	These results suggest that the mammalian Pygo genes are significant modulators of canonical Wnt signaling in some, but not in all developing systems.
T9421	14133-14138	DT	denotes	These
T9422	14139-14146	NNS	denotes	results
T9423	14147-14154	VBP	denotes	suggest
T9424	14155-14159	IN	denotes	that
T9426	14160-14163	DT	denotes	the
T9428	14164-14173	JJ	denotes	mammalian
T9429	14174-14178	NN	denotes	Pygo
T9427	14179-14184	NNS	denotes	genes
T9425	14185-14188	VBP	denotes	are
T9430	14189-14200	JJ	denotes	significant
T9431	14201-14211	NNS	denotes	modulators
T9432	14212-14214	IN	denotes	of
T9433	14215-14224	JJ	denotes	canonical
T9435	14225-14228	NN	denotes	Wnt
T9434	14229-14238	NN	denotes	signaling
T9436	14239-14241	IN	denotes	in
T9437	14242-14246	DT	denotes	some
T9438	14246-14248	,	denotes	,
T9439	14248-14251	CC	denotes	but
T9440	14252-14255	RB	denotes	not
T9441	14256-14258	IN	denotes	in
T9442	14259-14262	DT	denotes	all
T9443	14263-14273	VBG	denotes	developing
T9444	14274-14281	NNS	denotes	systems
T9445	14281-14282	.	denotes	.
T9446	14282-14283	sentence	denotes
T24238	14293-14300	VBN	denotes	Reduced
T24240	14301-14310	JJ	denotes	canonical
T24241	14311-14314	NN	denotes	Wnt
T24239	14315-14324	NN	denotes	signaling
T24242	14325-14327	IN	denotes	in
T24243	14328-14333	NN	denotes	Pygo2
T24245	14334-14337	CC	denotes	and
T24246	14338-14343	NN	denotes	Pygo1
T24248	14343-14344	HYPH	denotes	/
T24247	14344-14349	NN	denotes	Pygo2
T24244	14350-14356	NN	denotes	mutant
T24249	14357-14364	NNS	denotes	embryos
T24250	14364-14365	.	denotes	.
T24251	14365-14448	sentence	denotes	E10.5 embryos, all with the BAT-gal transgene reporter of canonical Wnt signaling.
T24252	14366-14371	NN	denotes	E10.5
T24253	14372-14379	NNS	denotes	embryos
T24254	14379-14381	,	denotes	,
T24255	14381-14384	DT	denotes	all
T24256	14385-14389	IN	denotes	with
T24257	14390-14393	DT	denotes	the
T24259	14394-14397	NN	denotes	BAT
T24261	14397-14398	HYPH	denotes	-
T24260	14398-14401	NN	denotes	gal
T24262	14402-14411	NN	denotes	transgene
T24258	14412-14420	NN	denotes	reporter
T24263	14421-14423	IN	denotes	of
T24264	14424-14433	JJ	denotes	canonical
T24266	14434-14437	NN	denotes	Wnt
T24265	14438-14447	NN	denotes	signaling
T24267	14447-14448	.	denotes	.
T24268	14448-14639	sentence	denotes	(A) Pygo1+/+/Pygo2+/- embryos showed normal X-gal staining in the developing brain, pharyngeal pouches, otic vesicle, apical ectodermal ridges of the fore and hind limb buds, and in somites.
T24269	14449-14450	-LRB-	denotes	(
T24270	14450-14451	LS	denotes	A
T24272	14451-14452	-RRB-	denotes	)
T24273	14453-14458	NN	denotes	Pygo1
T24275	14458-14459	SYM	denotes	+
T24276	14459-14460	HYPH	denotes	/
T24277	14460-14461	SYM	denotes	+
T24278	14461-14462	HYPH	denotes	/
T24279	14462-14467	NN	denotes	Pygo2
T24280	14467-14468	SYM	denotes	+
T24281	14468-14469	HYPH	denotes	/
T24282	14469-14470	SYM	denotes	-
T24274	14471-14478	NNS	denotes	embryos
T24271	14479-14485	VBD	denotes	showed
T24283	14486-14492	JJ	denotes	normal
T24285	14493-14494	NN	denotes	X
T24287	14494-14495	HYPH	denotes	-
T24286	14495-14498	NN	denotes	gal
T24284	14499-14507	NN	denotes	staining
T24288	14508-14510	IN	denotes	in
T24289	14511-14514	DT	denotes	the
T24291	14515-14525	VBG	denotes	developing
T24290	14526-14531	NN	denotes	brain
T24292	14531-14533	,	denotes	,
T24293	14533-14543	JJ	denotes	pharyngeal
T24294	14544-14551	NNS	denotes	pouches
T24295	14551-14553	,	denotes	,
T24296	14553-14557	JJ	denotes	otic
T24297	14558-14565	NN	denotes	vesicle
T24298	14565-14567	,	denotes	,
T24299	14567-14573	JJ	denotes	apical
T24301	14574-14584	JJ	denotes	ectodermal
T24300	14585-14591	NNS	denotes	ridges
T24302	14592-14594	IN	denotes	of
T24303	14595-14598	DT	denotes	the
T24305	14599-14603	JJ	denotes	fore
T24307	14604-14607	CC	denotes	and
T24308	14608-14612	JJ	denotes	hind
T24306	14613-14617	NN	denotes	limb
T24304	14618-14622	NNS	denotes	buds
T24309	14622-14624	,	denotes	,
T24310	14624-14627	CC	denotes	and
T24311	14628-14630	IN	denotes	in
T24312	14631-14638	NNS	denotes	somites
T24313	14638-14639	.	denotes	.
T24314	14639-14849	sentence	denotes	(B) Pygo1+/-/Pygo2-/- embryos, with loss of both Pygo2 alleles, showed reduced but not absent BAT-gal reporter expression in many developing structures, including pharyngeal pouches, otic vesicle, and somites.
T24315	14640-14641	-LRB-	denotes	(
T24316	14641-14642	LS	denotes	B
T24318	14642-14643	-RRB-	denotes	)
T24319	14644-14649	NN	denotes	Pygo1
T24321	14649-14650	SYM	denotes	+
T24322	14650-14651	HYPH	denotes	/
T24323	14651-14652	SYM	denotes	-
T24324	14652-14653	HYPH	denotes	/
T24325	14653-14658	NN	denotes	Pygo2
T24326	14658-14659	SYM	denotes	-
T24327	14659-14660	HYPH	denotes	/
T24328	14660-14661	SYM	denotes	-
T24320	14662-14669	NNS	denotes	embryos
T24329	14669-14671	,	denotes	,
T24330	14671-14675	IN	denotes	with
T24331	14676-14680	NN	denotes	loss
T24332	14681-14683	IN	denotes	of
T24333	14684-14688	DT	denotes	both
T24335	14689-14694	NN	denotes	Pygo2
T24334	14695-14702	NNS	denotes	alleles
T24336	14702-14704	,	denotes	,
T24317	14704-14710	VBD	denotes	showed
T24337	14711-14718	JJ	denotes	reduced
T24339	14719-14722	CC	denotes	but
T24340	14723-14726	RB	denotes	not
T24341	14727-14733	JJ	denotes	absent
T24342	14734-14737	NN	denotes	BAT
T24344	14737-14738	HYPH	denotes	-
T24343	14738-14741	NN	denotes	gal
T24338	14742-14750	NN	denotes	reporter
T24345	14751-14761	NN	denotes	expression
T24346	14762-14764	IN	denotes	in
T24347	14765-14769	JJ	denotes	many
T24349	14770-14780	VBG	denotes	developing
T24348	14781-14791	NNS	denotes	structures
T24350	14791-14793	,	denotes	,
T24351	14793-14802	VBG	denotes	including
T24352	14803-14813	JJ	denotes	pharyngeal
T24353	14814-14821	NNS	denotes	pouches
T24354	14821-14823	,	denotes	,
T24355	14823-14827	JJ	denotes	otic
T24356	14828-14835	NN	denotes	vesicle
T24357	14835-14837	,	denotes	,
T24358	14837-14840	CC	denotes	and
T24359	14841-14848	NNS	denotes	somites
T24360	14848-14849	.	denotes	.
T24361	14849-14982	sentence	denotes	(C) Pygo1-/-/Pygo2+/- embryo, with mutation of both Pygo1 alleles, but one wild-type Pygo2 allele, showed normal BAT-gal expression.
T24362	14850-14851	-LRB-	denotes	(
T24363	14851-14852	LS	denotes	C
T24365	14852-14853	-RRB-	denotes	)
T24366	14854-14859	NN	denotes	Pygo1
T24368	14859-14860	SYM	denotes	-
T24369	14860-14861	HYPH	denotes	/
T24370	14861-14862	SYM	denotes	-
T24371	14862-14863	HYPH	denotes	/
T24372	14863-14868	NN	denotes	Pygo2
T24373	14868-14869	SYM	denotes	+
T24374	14869-14870	HYPH	denotes	/
T24375	14870-14871	SYM	denotes	-
T24367	14872-14878	NN	denotes	embryo
T24376	14878-14880	,	denotes	,
T24377	14880-14884	IN	denotes	with
T24378	14885-14893	NN	denotes	mutation
T24379	14894-14896	IN	denotes	of
T24380	14897-14901	DT	denotes	both
T24382	14902-14907	NN	denotes	Pygo1
T24381	14908-14915	NNS	denotes	alleles
T24383	14915-14917	,	denotes	,
T24384	14917-14920	CC	denotes	but
T24385	14921-14924	CD	denotes	one
T24387	14925-14929	JJ	denotes	wild
T24389	14929-14930	HYPH	denotes	-
T24388	14930-14934	NN	denotes	type
T24390	14935-14940	NN	denotes	Pygo2
T24386	14941-14947	NN	denotes	allele
T24391	14947-14949	,	denotes	,
T24364	14949-14955	VBD	denotes	showed
T24392	14956-14962	JJ	denotes	normal
T24394	14963-14966	NN	denotes	BAT
T24396	14966-14967	HYPH	denotes	-
T24395	14967-14970	NN	denotes	gal
T24393	14971-14981	NN	denotes	expression
T24397	14981-14982	.	denotes	.
T24398	14982-15130	sentence	denotes	(D) Double-homozygous mutant Pygo1-/-/Pygo2-/- embryos still showed some remaining BAT-gal expression, suggesting residual canonical Wnt signaling.
T24399	14983-14984	-LRB-	denotes	(
T24400	14984-14985	LS	denotes	D
T24402	14985-14986	-RRB-	denotes	)
T24403	14987-14993	JJ	denotes	Double
T24405	14993-14994	HYPH	denotes	-
T24404	14994-15004	JJ	denotes	homozygous
T24407	15005-15011	NN	denotes	mutant
T24408	15012-15017	JJ	denotes	Pygo1
T24409	15017-15018	SYM	denotes	-
T24410	15018-15019	HYPH	denotes	/
T24411	15019-15020	SYM	denotes	-
T24412	15020-15021	HYPH	denotes	/
T24413	15021-15026	NN	denotes	Pygo2
T24414	15026-15027	SYM	denotes	-
T24415	15027-15028	HYPH	denotes	/
T24416	15028-15029	SYM	denotes	-
T24406	15030-15037	NNS	denotes	embryos
T24417	15038-15043	RB	denotes	still
T24401	15044-15050	VBD	denotes	showed
T24418	15051-15055	DT	denotes	some
T24420	15056-15065	VBG	denotes	remaining
T24421	15066-15069	NN	denotes	BAT
T24423	15069-15070	HYPH	denotes	-
T24422	15070-15073	NN	denotes	gal
T24419	15074-15084	NN	denotes	expression
T24424	15084-15086	,	denotes	,
T24425	15086-15096	VBG	denotes	suggesting
T24426	15097-15105	JJ	denotes	residual
T24428	15106-15115	JJ	denotes	canonical
T24429	15116-15119	NN	denotes	Wnt
T24427	15120-15129	NN	denotes	signaling
T24430	15129-15130	.	denotes	.
T24431	15130-15353	sentence	denotes	Embryos in panels (A) and (B) were from the same litter and were processed in parallel, while embryos in (C) and (D) were from a separate litter, also processed in parallel, and were slightly more developmentally advanced.
T24432	15131-15138	NNS	denotes	Embryos
T24434	15139-15141	IN	denotes	in
T24435	15142-15148	NNS	denotes	panels
T24437	15149-15150	-LRB-	denotes	(
T24436	15150-15151	NN	denotes	A
T24438	15151-15152	-RRB-	denotes	)
T24439	15153-15156	CC	denotes	and
T24440	15157-15158	-LRB-	denotes	(
T24441	15158-15159	NN	denotes	B
T24442	15159-15160	-RRB-	denotes	)
T24433	15161-15165	VBD	denotes	were
T24443	15166-15170	IN	denotes	from
T24444	15171-15174	DT	denotes	the
T24446	15175-15179	JJ	denotes	same
T24445	15180-15186	NN	denotes	litter
T24447	15187-15190	CC	denotes	and
T24448	15191-15195	VBD	denotes	were
T24449	15196-15205	VBN	denotes	processed
T24450	15206-15208	IN	denotes	in
T24451	15209-15217	NN	denotes	parallel
T24452	15217-15219	,	denotes	,
T24453	15219-15224	IN	denotes	while
T24455	15225-15232	NNS	denotes	embryos
T24456	15233-15235	IN	denotes	in
T24457	15236-15237	-LRB-	denotes	(
T24458	15237-15238	NN	denotes	C
T24459	15238-15239	-RRB-	denotes	)
T24460	15240-15243	CC	denotes	and
T24461	15244-15245	-LRB-	denotes	(
T24462	15245-15246	NN	denotes	D
T24463	15246-15247	-RRB-	denotes	)
T24454	15248-15252	VBD	denotes	were
T24464	15253-15257	IN	denotes	from
T24465	15258-15259	DT	denotes	a
T24467	15260-15268	JJ	denotes	separate
T24466	15269-15275	NN	denotes	litter
T24468	15275-15277	,	denotes	,
T24469	15277-15281	RB	denotes	also
T24470	15282-15291	VBN	denotes	processed
T24471	15292-15294	IN	denotes	in
T24472	15295-15303	NN	denotes	parallel
T24473	15303-15305	,	denotes	,
T24474	15305-15308	CC	denotes	and
T24475	15309-15313	VBD	denotes	were
T24476	15314-15322	RB	denotes	slightly
T24477	15323-15327	RBR	denotes	more
T24479	15328-15343	RB	denotes	developmentally
T24478	15344-15352	JJ	denotes	advanced
T24480	15352-15353	.	denotes	.
T24481	15353-15402	sentence	denotes	Original magnification (A, B) × 20; (C, D) × 16.
T24482	15354-15362	JJ	denotes	Original
T24483	15363-15376	NN	denotes	magnification
T24485	15377-15378	-LRB-	denotes	(
T24487	15378-15379	NN	denotes	A
T24488	15379-15381	,	denotes	,
T24486	15381-15382	NN	denotes	B
T24489	15382-15383	-RRB-	denotes	)
T24490	15384-15385	SYM	denotes	×
T24484	15386-15388	CD	denotes	20
T24491	15388-15389	:	denotes	;
T24492	15390-15391	-LRB-	denotes	(
T24494	15391-15392	NN	denotes	C
T24495	15392-15394	,	denotes	,
T24493	15394-15395	NN	denotes	D
T24497	15395-15396	-RRB-	denotes	)
T24498	15397-15398	SYM	denotes	×
T24496	15399-15401	CD	denotes	16
T24499	15401-15402	.	denotes	.
T9448	15403-15404	NN	denotes	W
T9447	15403-15405	sentence	denotes	We
T9449	15404-15405	NNS	denotes	e
T9450	15405-15411	sentence	denotes	also
T9451	15406-15408	DT	denotes	al
T9453	15408-15409	NN	denotes	s
T9452	15409-15410	NN	denotes	o
T9455	15411-15412	NNS	denotes	e
T9454	15411-15412	sentence	denotes	e
T9457	15412-15413	NN	denotes	x
T9456	15412-15416	sentence	denotes	xami
T9458	15413-15414	JJ	denotes	a
T9459	15414-15416	NNS	denotes	mi
T9461	15416-15417	NN	denotes	n
T9460	15416-15430	sentence	denotes	ned BAT-gal re
T9462	15417-15418	NNS	denotes	e
T9463	15418-15419	JJ	denotes	d
T9465	15420-15423	NN	denotes	BAT
T9467	15423-15424	HYPH	denotes	-
T9466	15424-15427	NN	denotes	gal
T9464	15428-15430	NN	denotes	re
T9469	15430-15432	NN	denotes	po
T9468	15430-15434	sentence	denotes	port
T9470	15432-15433	JJ	denotes	r
T9471	15433-15434	NN	denotes	t
T9474	15434-15435	NNS	denotes	e
T9472	15434-15438	sentence	denotes	er e
T9473	15435-15436	VBD	denotes	r
T9475	15437-15438	DT	denotes	e
T9477	15438-15439	VBD	denotes	x
T9476	15438-15515	sentence	denotes	xpression in more detail in the developing urogenital system of Pygo mutants.
T9478	15439-15442	NN	denotes	pre
T9479	15442-15447	NN	denotes	ssion
T9480	15448-15450	IN	denotes	in
T9481	15451-15455	JJR	denotes	more
T9482	15456-15462	NN	denotes	detail
T9483	15463-15465	IN	denotes	in
T9484	15466-15469	DT	denotes	the
T9486	15470-15480	VBG	denotes	developing
T9487	15481-15491	JJ	denotes	urogenital
T9485	15492-15498	NN	denotes	system
T9488	15499-15501	IN	denotes	of
T9489	15502-15506	NN	denotes	Pygo
T9490	15507-15514	NNS	denotes	mutants
T9491	15514-15515	.	denotes	.
T9492	15515-15618	sentence	denotes	The results suggested that the Pygo2 gene is required for canonical Wnt signaling in the nephric duct.
T9493	15516-15519	DT	denotes	The
T9494	15520-15527	NNS	denotes	results
T9495	15528-15537	VBD	denotes	suggested
T9496	15538-15542	IN	denotes	that
T9498	15543-15546	DT	denotes	the
T9500	15547-15552	NN	denotes	Pygo2
T9499	15553-15557	NN	denotes	gene
T9501	15558-15560	VBZ	denotes	is
T9497	15561-15569	VBN	denotes	required
T9502	15570-15573	IN	denotes	for
T9503	15574-15583	JJ	denotes	canonical
T9505	15584-15587	NN	denotes	Wnt
T9504	15588-15597	NN	denotes	signaling
T9506	15598-15600	IN	denotes	in
T9507	15601-15604	DT	denotes	the
T9509	15605-15612	JJ	denotes	nephric
T9508	15613-15617	NN	denotes	duct
T9510	15617-15618	.	denotes	.
T9511	15618-15804	sentence	denotes	Both Pygo2 null and Pygo1/Pygo2 double-null E10.5 embryos showed an absence of reporter expression in the nephric duct, while control littermates showed strong expression (Figure 6A–C).
T9512	15619-15623	CC	denotes	Both
T9514	15624-15629	NN	denotes	Pygo2
T9513	15630-15634	JJ	denotes	null
T9516	15635-15638	CC	denotes	and
T9517	15639-15644	NN	denotes	Pygo1
T9519	15644-15645	HYPH	denotes	/
T9518	15645-15650	NN	denotes	Pygo2
T9521	15651-15657	JJ	denotes	double
T9522	15657-15658	HYPH	denotes	-
T9520	15658-15662	JJ	denotes	null
T9523	15663-15668	NN	denotes	E10.5
T9515	15669-15676	NNS	denotes	embryos
T9524	15677-15683	VBD	denotes	showed
T9525	15684-15686	DT	denotes	an
T9526	15687-15694	NN	denotes	absence
T9527	15695-15697	IN	denotes	of
T9528	15698-15706	NN	denotes	reporter
T9529	15707-15717	NN	denotes	expression
T9530	15718-15720	IN	denotes	in
T9531	15721-15724	DT	denotes	the
T9533	15725-15732	JJ	denotes	nephric
T9532	15733-15737	NN	denotes	duct
T9534	15737-15739	,	denotes	,
T9535	15739-15744	IN	denotes	while
T9537	15745-15752	NN	denotes	control
T9538	15753-15764	NNS	denotes	littermates
T9536	15765-15771	VBD	denotes	showed
T9539	15772-15778	JJ	denotes	strong
T9540	15779-15789	NN	denotes	expression
T9541	15790-15791	-LRB-	denotes	(
T9543	15791-15797	NN	denotes	Figure
T9542	15798-15800	NN	denotes	6A
T9544	15800-15801	SYM	denotes	–
T9545	15801-15802	NN	denotes	C
T9546	15802-15803	-RRB-	denotes	)
T9547	15803-15804	.	denotes	.
T9548	15804-16000	sentence	denotes	In Pygo1/Pygo2 double-null mutants, the nephric duct did form, however, and give rise to the ureteric bud outgrowth, which showed reduced but not absent BAT-gal reporter expression (Figure 6A–C).
T9549	15805-15807	IN	denotes	In
T9551	15808-15813	NN	denotes	Pygo1
T9553	15813-15814	HYPH	denotes	/
T9552	15814-15819	NN	denotes	Pygo2
T9555	15820-15826	JJ	denotes	double
T9556	15826-15827	HYPH	denotes	-
T9554	15827-15831	JJ	denotes	null
T9557	15832-15839	NNS	denotes	mutants
T9558	15839-15841	,	denotes	,
T9559	15841-15844	DT	denotes	the
T9561	15845-15852	JJ	denotes	nephric
T9560	15853-15857	NN	denotes	duct
T9562	15858-15861	VBD	denotes	did
T9550	15862-15866	VB	denotes	form
T9563	15866-15868	,	denotes	,
T9564	15868-15875	RB	denotes	however
T9565	15875-15877	,	denotes	,
T9566	15877-15880	CC	denotes	and
T9567	15881-15885	VB	denotes	give
T9568	15886-15890	NN	denotes	rise
T9569	15891-15893	IN	denotes	to
T9570	15894-15897	DT	denotes	the
T9572	15898-15906	JJ	denotes	ureteric
T9573	15907-15910	NN	denotes	bud
T9571	15911-15920	NN	denotes	outgrowth
T9574	15920-15922	,	denotes	,
T9575	15922-15927	WDT	denotes	which
T9576	15928-15934	VBD	denotes	showed
T9577	15935-15942	VBN	denotes	reduced
T9579	15943-15946	CC	denotes	but
T9580	15947-15950	RB	denotes	not
T9581	15951-15957	JJ	denotes	absent
T9582	15958-15961	NN	denotes	BAT
T9584	15961-15962	HYPH	denotes	-
T9583	15962-15965	NN	denotes	gal
T9585	15966-15974	NN	denotes	reporter
T9578	15975-15985	NN	denotes	expression
T9586	15986-15987	-LRB-	denotes	(
T9588	15987-15993	NN	denotes	Figure
T9587	15994-15996	NN	denotes	6A
T9589	15996-15997	SYM	denotes	–
T9590	15997-15998	NN	denotes	C
T9591	15998-15999	-RRB-	denotes	)
T9592	15999-16000	.	denotes	.
T9593	16000-16001	sentence	denotes
T25934	16011-16016	NN	denotes	Pygo2
T25936	16017-16019	VBZ	denotes	is
T25935	16020-16028	VBN	denotes	required
T25937	16029-16032	IN	denotes	for
T25938	16033-16036	NN	denotes	BAT
T25940	16036-16037	HYPH	denotes	-
T25939	16037-16040	NN	denotes	gal
T25941	16041-16049	NN	denotes	reporter
T25942	16050-16060	NN	denotes	expression
T25943	16061-16063	IN	denotes	in
T25944	16064-16072	JJ	denotes	ureteric
T25945	16073-16076	NN	denotes	bud
T25947	16076-16077	HYPH	denotes	-
T25946	16077-16084	VBN	denotes	derived
T25948	16085-16095	NNS	denotes	structures
T25949	16096-16098	IN	denotes	of
T25950	16099-16102	DT	denotes	the
T25952	16103-16113	VBG	denotes	developing
T25951	16114-16120	NN	denotes	kidney
T25953	16120-16121	.	denotes	.
T25954	16121-16231	sentence	denotes	X-Gal staining of BAT-gal transgenic (A-C) E10.5, and (E-L) E13.5 urogenital tracts, and (M-O) E18.5 kidneys.
T25955	16122-16123	NN	denotes	X
T25957	16123-16124	HYPH	denotes	-
T25956	16124-16127	NN	denotes	Gal
T25958	16128-16136	NN	denotes	staining
T25959	16137-16139	IN	denotes	of
T25960	16140-16143	NN	denotes	BAT
T25962	16143-16144	HYPH	denotes	-
T25961	16144-16147	NN	denotes	gal
T25963	16148-16158	JJ	denotes	transgenic
T25965	16159-16160	-LRB-	denotes	(
T25966	16160-16161	NN	denotes	A
T25968	16161-16162	SYM	denotes	-
T25969	16162-16163	NN	denotes	C
T25970	16163-16164	-RRB-	denotes	)
T25967	16165-16170	NN	denotes	E10.5
T25971	16170-16172	,	denotes	,
T25972	16172-16175	CC	denotes	and
T25973	16176-16177	-LRB-	denotes	(
T25974	16177-16178	NN	denotes	E
T25976	16178-16179	SYM	denotes	-
T25977	16179-16180	NN	denotes	L
T25978	16180-16181	-RRB-	denotes	)
T25975	16182-16187	NN	denotes	E13.5
T25979	16188-16198	JJ	denotes	urogenital
T25964	16199-16205	NNS	denotes	tracts
T25980	16205-16207	,	denotes	,
T25981	16207-16210	CC	denotes	and
T25982	16211-16212	-LRB-	denotes	(
T25983	16212-16213	NN	denotes	M
T25985	16213-16214	SYM	denotes	-
T25986	16214-16215	NN	denotes	O
T25987	16215-16216	-RRB-	denotes	)
T25988	16217-16222	NN	denotes	E18.5
T25984	16223-16230	NNS	denotes	kidneys
T25989	16230-16231	.	denotes	.
T25990	16231-16291	sentence	denotes	(A) Pygo1-/-/Pygo2+/+ (left) and Pygo1+/-/Pygo2-/- (right).
T25991	16232-16233	-LRB-	denotes	(
T25992	16233-16234	LS	denotes	A
T25994	16234-16235	-RRB-	denotes	)
T25993	16236-16241	NN	denotes	Pygo1
T25995	16241-16242	SYM	denotes	-
T25996	16242-16243	HYPH	denotes	/
T25997	16243-16244	SYM	denotes	-
T25998	16244-16245	HYPH	denotes	/
T25999	16245-16250	NN	denotes	Pygo2
T26000	16250-16251	SYM	denotes	+
T26001	16251-16252	HYPH	denotes	/
T26002	16252-16253	SYM	denotes	+
T26003	16254-16255	-LRB-	denotes	(
T26004	16255-16259	JJ	denotes	left
T26005	16259-16260	-RRB-	denotes	)
T26006	16261-16264	CC	denotes	and
T26007	16265-16270	NN	denotes	Pygo1
T26008	16270-16271	SYM	denotes	+
T26009	16271-16272	HYPH	denotes	/
T26010	16272-16273	SYM	denotes	-
T26011	16273-16274	HYPH	denotes	/
T26012	16274-16279	NN	denotes	Pygo2
T26013	16279-16280	SYM	denotes	-
T26014	16280-16281	HYPH	denotes	/
T26015	16281-16282	SYM	denotes	-
T26016	16283-16284	-LRB-	denotes	(
T26017	16284-16289	JJ	denotes	right
T26018	16289-16290	-RRB-	denotes	)
T26019	16290-16291	.	denotes	.
T26020	16291-16458	sentence	denotes	Note the loss of reporter activity in the nephric duct (black arrowhead) and reduction of staining in the ureteric bud (white arrow) of the Pygo2 null embryo (Right).
T26021	16292-16296	VB	denotes	Note
T26022	16297-16300	DT	denotes	the
T26023	16301-16305	NN	denotes	loss
T26024	16306-16308	IN	denotes	of
T26025	16309-16317	NN	denotes	reporter
T26026	16318-16326	NN	denotes	activity
T26027	16327-16329	IN	denotes	in
T26028	16330-16333	DT	denotes	the
T26030	16334-16341	JJ	denotes	nephric
T26029	16342-16346	NN	denotes	duct
T26031	16347-16348	-LRB-	denotes	(
T26033	16348-16353	JJ	denotes	black
T26032	16354-16363	NN	denotes	arrowhead
T26034	16363-16364	-RRB-	denotes	)
T26035	16365-16368	CC	denotes	and
T26036	16369-16378	NN	denotes	reduction
T26037	16379-16381	IN	denotes	of
T26038	16382-16390	NN	denotes	staining
T26039	16391-16393	IN	denotes	in
T26040	16394-16397	DT	denotes	the
T26042	16398-16406	JJ	denotes	ureteric
T26041	16407-16410	NN	denotes	bud
T26043	16411-16412	-LRB-	denotes	(
T26045	16412-16417	JJ	denotes	white
T26044	16418-16423	NN	denotes	arrow
T26046	16423-16424	-RRB-	denotes	)
T26047	16425-16427	IN	denotes	of
T26048	16428-16431	DT	denotes	the
T26050	16432-16437	NN	denotes	Pygo2
T26051	16438-16442	JJ	denotes	null
T26049	16443-16449	NN	denotes	embryo
T26052	16450-16451	-LRB-	denotes	(
T26053	16451-16456	JJ	denotes	Right
T26054	16456-16457	-RRB-	denotes	)
T26055	16457-16458	.	denotes	.
T26056	16458-16594	sentence	denotes	(B) Pygo1-/-/Pygo2+/- E10.5 embryo with BAT-gal reporter activity in the nephric duct (black arrowhead) and ureteric bud (white arrow).
T26057	16459-16460	-LRB-	denotes	(
T26058	16460-16461	LS	denotes	B
T26060	16461-16462	-RRB-	denotes	)
T26061	16463-16468	NN	denotes	Pygo1
T26062	16468-16469	SYM	denotes	-
T26063	16469-16470	HYPH	denotes	/
T26064	16470-16471	SYM	denotes	-
T26065	16471-16472	HYPH	denotes	/
T26066	16472-16477	NN	denotes	Pygo2
T26067	16477-16478	SYM	denotes	+
T26068	16478-16479	HYPH	denotes	/
T26069	16479-16480	SYM	denotes	-
T26070	16481-16486	NN	denotes	E10.5
T26059	16487-16493	NN	denotes	embryo
T26071	16494-16498	IN	denotes	with
T26072	16499-16502	NN	denotes	BAT
T26074	16502-16503	HYPH	denotes	-
T26073	16503-16506	NN	denotes	gal
T26075	16507-16515	NN	denotes	reporter
T26076	16516-16524	NN	denotes	activity
T26077	16525-16527	IN	denotes	in
T26078	16528-16531	DT	denotes	the
T26080	16532-16539	JJ	denotes	nephric
T26079	16540-16544	NN	denotes	duct
T26081	16545-16546	-LRB-	denotes	(
T26083	16546-16551	JJ	denotes	black
T26082	16552-16561	NN	denotes	arrowhead
T26084	16561-16562	-RRB-	denotes	)
T26085	16563-16566	CC	denotes	and
T26086	16567-16575	JJ	denotes	ureteric
T26087	16576-16579	NN	denotes	bud
T26088	16580-16581	-LRB-	denotes	(
T26090	16581-16586	JJ	denotes	white
T26089	16587-16592	NN	denotes	arrow
T26091	16592-16593	-RRB-	denotes	)
T26092	16593-16594	.	denotes	.
T26093	16594-16720	sentence	denotes	(C) Pygo1-/-/Pygo-/- embryo, with reporter expression lost in the nephric duct and reduced in the ureteric bud (white arrow).
T26094	16595-16596	-LRB-	denotes	(
T26095	16596-16597	LS	denotes	C
T26097	16597-16598	-RRB-	denotes	)
T26098	16599-16604	NN	denotes	Pygo1
T26099	16604-16605	SYM	denotes	-
T26100	16605-16606	HYPH	denotes	/
T26101	16606-16607	SYM	denotes	-
T26102	16607-16608	HYPH	denotes	/
T26103	16608-16612	NN	denotes	Pygo
T26104	16612-16613	SYM	denotes	-
T26105	16613-16614	HYPH	denotes	/
T26106	16614-16615	SYM	denotes	-
T26096	16616-16622	NN	denotes	embryo
T26107	16622-16624	,	denotes	,
T26108	16624-16628	IN	denotes	with
T26110	16629-16637	NN	denotes	reporter
T26111	16638-16648	NN	denotes	expression
T26109	16649-16653	JJ	denotes	lost
T26112	16654-16656	IN	denotes	in
T26113	16657-16660	DT	denotes	the
T26115	16661-16668	JJ	denotes	nephric
T26114	16669-16673	NN	denotes	duct
T26116	16674-16677	CC	denotes	and
T26117	16678-16685	VBN	denotes	reduced
T26118	16686-16688	IN	denotes	in
T26119	16689-16692	DT	denotes	the
T26121	16693-16701	JJ	denotes	ureteric
T26120	16702-16705	NN	denotes	bud
T26122	16706-16707	-LRB-	denotes	(
T26124	16707-16712	JJ	denotes	white
T26123	16713-16718	NN	denotes	arrow
T26125	16718-16719	-RRB-	denotes	)
T26126	16719-16720	.	denotes	.
T26127	16720-16900	sentence	denotes	(D) Control background X-gal staining of a urogenital tract from an E13.5 embryo without the BAT-gal transgene (Non-Tg), showing absence of endogenous beta-galactosidase activity.
T26128	16721-16722	-LRB-	denotes	(
T26129	16722-16723	LS	denotes	D
T26131	16723-16724	-RRB-	denotes	)
T26132	16725-16732	NN	denotes	Control
T26133	16733-16743	NN	denotes	background
T26134	16744-16745	NN	denotes	X
T26136	16745-16746	HYPH	denotes	-
T26135	16746-16749	NN	denotes	gal
T26130	16750-16758	NN	denotes	staining
T26137	16759-16761	IN	denotes	of
T26138	16762-16763	DT	denotes	a
T26140	16764-16774	JJ	denotes	urogenital
T26139	16775-16780	NN	denotes	tract
T26141	16781-16785	IN	denotes	from
T26142	16786-16788	DT	denotes	an
T26144	16789-16794	NN	denotes	E13.5
T26143	16795-16801	NN	denotes	embryo
T26145	16802-16809	IN	denotes	without
T26146	16810-16813	DT	denotes	the
T26148	16814-16817	NN	denotes	BAT
T26150	16817-16818	HYPH	denotes	-
T26149	16818-16821	NN	denotes	gal
T26147	16822-16831	NN	denotes	transgene
T26151	16832-16833	-LRB-	denotes	(
T26152	16833-16839	NN	denotes	Non-Tg
T26153	16839-16840	-RRB-	denotes	)
T26154	16840-16842	,	denotes	,
T26155	16842-16849	VBG	denotes	showing
T26156	16850-16857	NN	denotes	absence
T26157	16858-16860	IN	denotes	of
T26158	16861-16871	JJ	denotes	endogenous
T26160	16872-16876	NN	denotes	beta
T26162	16876-16877	HYPH	denotes	-
T26161	16877-16890	NN	denotes	galactosidase
T26159	16891-16899	NN	denotes	activity
T26163	16899-16900	.	denotes	.
T26164	16900-17064	sentence	denotes	(E) Pygo1+/-/Pygo2+/+, with BAT-gal reporter activity in the ureteric compartment of the developing kidney, including the ureteric tips, ureteric tree, and ureter.
T26165	16901-16902	-LRB-	denotes	(
T26166	16902-16903	LS	denotes	E
T26168	16903-16904	-RRB-	denotes	)
T26167	16905-16910	NN	denotes	Pygo1
T26169	16910-16911	SYM	denotes	+
T26170	16911-16912	HYPH	denotes	/
T26171	16912-16913	SYM	denotes	-
T26172	16913-16914	HYPH	denotes	/
T26173	16914-16919	NN	denotes	Pygo2
T26174	16919-16920	SYM	denotes	+
T26175	16920-16921	HYPH	denotes	/
T26176	16921-16922	SYM	denotes	+
T26177	16922-16924	,	denotes	,
T26178	16924-16928	IN	denotes	with
T26179	16929-16932	NN	denotes	BAT
T26181	16932-16933	HYPH	denotes	-
T26180	16933-16936	NN	denotes	gal
T26182	16937-16945	NN	denotes	reporter
T26183	16946-16954	NN	denotes	activity
T26184	16955-16957	IN	denotes	in
T26185	16958-16961	DT	denotes	the
T26187	16962-16970	JJ	denotes	ureteric
T26186	16971-16982	NN	denotes	compartment
T26188	16983-16985	IN	denotes	of
T26189	16986-16989	DT	denotes	the
T26191	16990-17000	VBG	denotes	developing
T26190	17001-17007	NN	denotes	kidney
T26192	17007-17009	,	denotes	,
T26193	17009-17018	VBG	denotes	including
T26194	17019-17022	DT	denotes	the
T26196	17023-17031	JJ	denotes	ureteric
T26195	17032-17036	NNS	denotes	tips
T26197	17036-17038	,	denotes	,
T26198	17038-17046	JJ	denotes	ureteric
T26199	17047-17051	NN	denotes	tree
T26200	17051-17053	,	denotes	,
T26201	17053-17056	CC	denotes	and
T26202	17057-17063	NN	denotes	ureter
T26203	17063-17064	.	denotes	.
T26204	17064-17167	sentence	denotes	(F) Pygo1+/-/Pygo2-/-, with marked reduction of BAT-gal reporter activity in the ureteric compartment.
T26205	17065-17066	-LRB-	denotes	(
T26206	17066-17067	LS	denotes	F
T26208	17067-17068	-RRB-	denotes	)
T26207	17069-17074	NN	denotes	Pygo1
T26209	17074-17075	SYM	denotes	+
T26210	17075-17076	HYPH	denotes	/
T26211	17076-17077	SYM	denotes	-
T26212	17077-17078	HYPH	denotes	/
T26213	17078-17083	NN	denotes	Pygo2
T26214	17083-17084	SYM	denotes	-
T26215	17084-17085	HYPH	denotes	/
T26216	17085-17086	SYM	denotes	-
T26217	17086-17088	,	denotes	,
T26218	17088-17092	IN	denotes	with
T26219	17093-17099	JJ	denotes	marked
T26220	17100-17109	NN	denotes	reduction
T26221	17110-17112	IN	denotes	of
T26222	17113-17116	NN	denotes	BAT
T26224	17116-17117	HYPH	denotes	-
T26223	17117-17120	NN	denotes	gal
T26225	17121-17129	NN	denotes	reporter
T26226	17130-17138	NN	denotes	activity
T26227	17139-17141	IN	denotes	in
T26228	17142-17145	DT	denotes	the
T26230	17146-17154	JJ	denotes	ureteric
T26229	17155-17166	NN	denotes	compartment
T26231	17166-17167	.	denotes	.
T26232	17167-17280	sentence	denotes	(G) Pygo1+/-/Pygo2+/-, with reporter expression in the paramesonephric duct (white arrowhead) and ureteric tree.
T26233	17168-17169	-LRB-	denotes	(
T26234	17169-17170	LS	denotes	G
T26236	17170-17171	-RRB-	denotes	)
T26235	17172-17177	NN	denotes	Pygo1
T26237	17177-17178	SYM	denotes	+
T26238	17178-17179	HYPH	denotes	/
T26239	17179-17180	SYM	denotes	-
T26240	17180-17181	HYPH	denotes	/
T26241	17181-17186	NN	denotes	Pygo2
T26242	17186-17187	SYM	denotes	+
T26243	17187-17188	HYPH	denotes	/
T26244	17188-17189	SYM	denotes	-
T26245	17189-17191	,	denotes	,
T26246	17191-17195	IN	denotes	with
T26247	17196-17204	NN	denotes	reporter
T26248	17205-17215	NN	denotes	expression
T26249	17216-17218	IN	denotes	in
T26250	17219-17222	DT	denotes	the
T26252	17223-17238	JJ	denotes	paramesonephric
T26251	17239-17243	NN	denotes	duct
T26253	17244-17245	-LRB-	denotes	(
T26255	17245-17250	JJ	denotes	white
T26254	17251-17260	NN	denotes	arrowhead
T26256	17260-17261	-RRB-	denotes	)
T26257	17262-17265	CC	denotes	and
T26258	17266-17274	JJ	denotes	ureteric
T26259	17275-17279	NN	denotes	tree
T26260	17279-17280	.	denotes	.
T26261	17280-17471	sentence	denotes	(H, I) Pygo1-/-/Pygo2+/-, with (H) ventral view showing reporter expression in the paramesonephric duct (white arrowhead), and (I) dorsal view showing ureteric tree expression in the kidney.
T26262	17281-17282	-LRB-	denotes	(
T26263	17282-17283	LS	denotes	H
T26265	17283-17285	,	denotes	,
T26266	17285-17286	LS	denotes	I
T26267	17286-17287	-RRB-	denotes	)
T26264	17288-17293	NN	denotes	Pygo1
T26268	17293-17294	SYM	denotes	-
T26269	17294-17295	HYPH	denotes	/
T26270	17295-17296	SYM	denotes	-
T26271	17296-17297	HYPH	denotes	/
T26272	17297-17302	NN	denotes	Pygo2
T26273	17302-17303	SYM	denotes	+
T26274	17303-17304	HYPH	denotes	/
T26275	17304-17305	SYM	denotes	-
T26276	17305-17307	,	denotes	,
T26277	17307-17311	IN	denotes	with
T26278	17312-17313	-LRB-	denotes	(
T26279	17313-17314	LS	denotes	H
T26281	17314-17315	-RRB-	denotes	)
T26282	17316-17323	JJ	denotes	ventral
T26280	17324-17328	NN	denotes	view
T26283	17329-17336	VBG	denotes	showing
T26284	17337-17345	NN	denotes	reporter
T26285	17346-17356	NN	denotes	expression
T26286	17357-17359	IN	denotes	in
T26287	17360-17363	DT	denotes	the
T26289	17364-17379	JJ	denotes	paramesonephric
T26288	17380-17384	NN	denotes	duct
T26290	17385-17386	-LRB-	denotes	(
T26292	17386-17391	JJ	denotes	white
T26291	17392-17401	NN	denotes	arrowhead
T26293	17401-17402	-RRB-	denotes	)
T26294	17402-17404	,	denotes	,
T26295	17404-17407	CC	denotes	and
T26296	17408-17409	-LRB-	denotes	(
T26297	17409-17410	LS	denotes	I
T26299	17410-17411	-RRB-	denotes	)
T26300	17412-17418	JJ	denotes	dorsal
T26298	17419-17423	NN	denotes	view
T26301	17424-17431	VBG	denotes	showing
T26302	17432-17440	JJ	denotes	ureteric
T26303	17441-17445	NN	denotes	tree
T26304	17446-17456	NN	denotes	expression
T26305	17457-17459	IN	denotes	in
T26306	17460-17463	DT	denotes	the
T26307	17464-17470	NN	denotes	kidney
T26308	17470-17471	.	denotes	.
T26309	17471-17602	sentence	denotes	(J)Pygo1+/-/Pygo2+/-, a control processed in parallel with (K) and (L), with expression in ureteric tree and paramesonephric duct.
T26310	17472-17473	-LRB-	denotes	(
T26311	17473-17474	LS	denotes	J
T26313	17474-17475	-RRB-	denotes	)
T26312	17475-17480	NN	denotes	Pygo1
T26314	17480-17481	SYM	denotes	+
T26315	17481-17482	HYPH	denotes	/
T26316	17482-17483	SYM	denotes	-
T26317	17483-17484	HYPH	denotes	/
T26318	17484-17489	NN	denotes	Pygo2
T26319	17489-17490	SYM	denotes	+
T26320	17490-17491	HYPH	denotes	/
T26321	17491-17492	SYM	denotes	-
T26322	17492-17494	,	denotes	,
T26323	17494-17495	DT	denotes	a
T26324	17496-17503	NN	denotes	control
T26325	17504-17513	VBN	denotes	processed
T26326	17514-17516	IN	denotes	in
T26327	17517-17525	NN	denotes	parallel
T26328	17526-17530	IN	denotes	with
T26329	17531-17532	-LRB-	denotes	(
T26330	17532-17533	NN	denotes	K
T26331	17533-17534	-RRB-	denotes	)
T26332	17535-17538	CC	denotes	and
T26333	17539-17540	-LRB-	denotes	(
T26334	17540-17541	NN	denotes	L
T26335	17541-17542	-RRB-	denotes	)
T26336	17542-17544	,	denotes	,
T26337	17544-17548	IN	denotes	with
T26338	17549-17559	NN	denotes	expression
T26339	17560-17562	IN	denotes	in
T26340	17563-17571	JJ	denotes	ureteric
T26341	17572-17576	NN	denotes	tree
T26342	17577-17580	CC	denotes	and
T26343	17581-17596	JJ	denotes	paramesonephric
T26344	17597-17601	NN	denotes	duct
T26345	17601-17602	.	denotes	.
T26346	17602-17833	sentence	denotes	(K, L)Pygo1-/-/Pygo2-/-, reporter activity was lost in the paramesonephric duct (white arrowhead), and in the ureter and ureteric compartment of the developing kidneys (dashed circles), except for (K) a few faintly staining cells.
T26347	17603-17604	-LRB-	denotes	(
T26348	17604-17605	LS	denotes	K
T26350	17605-17607	,	denotes	,
T26351	17607-17608	LS	denotes	L
T26352	17608-17609	-RRB-	denotes	)
T26353	17609-17614	NN	denotes	Pygo1
T26355	17614-17615	SYM	denotes	-
T26356	17615-17616	HYPH	denotes	/
T26357	17616-17617	SYM	denotes	-
T26358	17617-17618	HYPH	denotes	/
T26359	17618-17623	NN	denotes	Pygo2
T26360	17623-17624	SYM	denotes	-
T26361	17624-17625	HYPH	denotes	/
T26362	17625-17626	SYM	denotes	-
T26363	17626-17628	,	denotes	,
T26354	17628-17636	NN	denotes	reporter
T26364	17637-17645	NN	denotes	activity
T26365	17646-17649	VBD	denotes	was
T26349	17650-17654	VBN	denotes	lost
T26366	17655-17657	IN	denotes	in
T26367	17658-17661	DT	denotes	the
T26369	17662-17677	JJ	denotes	paramesonephric
T26368	17678-17682	NN	denotes	duct
T26370	17683-17684	-LRB-	denotes	(
T26372	17684-17689	JJ	denotes	white
T26371	17690-17699	NN	denotes	arrowhead
T26373	17699-17700	-RRB-	denotes	)
T26374	17700-17702	,	denotes	,
T26375	17702-17705	CC	denotes	and
T26376	17706-17708	IN	denotes	in
T26377	17709-17712	DT	denotes	the
T26378	17713-17719	NN	denotes	ureter
T26379	17720-17723	CC	denotes	and
T26380	17724-17732	JJ	denotes	ureteric
T26381	17733-17744	NN	denotes	compartment
T26382	17745-17747	IN	denotes	of
T26383	17748-17751	DT	denotes	the
T26385	17752-17762	VBG	denotes	developing
T26384	17763-17770	NNS	denotes	kidneys
T26386	17771-17772	-LRB-	denotes	(
T26388	17772-17778	VBN	denotes	dashed
T26387	17779-17786	NNS	denotes	circles
T26389	17786-17787	-RRB-	denotes	)
T26390	17787-17789	,	denotes	,
T26391	17789-17795	IN	denotes	except
T26392	17796-17799	IN	denotes	for
T26393	17800-17801	-LRB-	denotes	(
T26394	17801-17802	NN	denotes	K
T26396	17802-17803	-RRB-	denotes	)
T26397	17804-17805	DT	denotes	a
T26398	17806-17809	JJ	denotes	few
T26399	17810-17817	RB	denotes	faintly
T26400	17818-17826	VBG	denotes	staining
T26395	17827-17832	NNS	denotes	cells
T26401	17832-17833	.	denotes	.
T26402	17833-17910	sentence	denotes	(M, N) Pygo1+/-/Pygo2+/- (left) and Pygo1-/-/Pygo2-/- (right) E18.5 kidneys.
T26403	17834-17835	-LRB-	denotes	(
T26404	17835-17836	LS	denotes	M
T26406	17836-17838	,	denotes	,
T26407	17838-17839	LS	denotes	N
T26408	17839-17840	-RRB-	denotes	)
T26409	17841-17846	NN	denotes	Pygo1
T26410	17846-17847	SYM	denotes	+
T26411	17847-17848	HYPH	denotes	/
T26412	17848-17849	SYM	denotes	-
T26413	17849-17850	HYPH	denotes	/
T26414	17850-17855	NN	denotes	Pygo2
T26415	17855-17856	SYM	denotes	+
T26416	17856-17857	HYPH	denotes	/
T26417	17857-17858	SYM	denotes	-
T26418	17859-17860	-LRB-	denotes	(
T26419	17860-17864	JJ	denotes	left
T26420	17864-17865	-RRB-	denotes	)
T26421	17866-17869	CC	denotes	and
T26422	17870-17875	NN	denotes	Pygo1
T26423	17875-17876	SYM	denotes	-
T26424	17876-17877	HYPH	denotes	/
T26425	17877-17878	SYM	denotes	-
T26426	17878-17879	HYPH	denotes	/
T26427	17879-17884	NN	denotes	Pygo2
T26428	17884-17885	SYM	denotes	-
T26429	17885-17886	HYPH	denotes	/
T26430	17886-17887	SYM	denotes	-
T26431	17888-17889	-LRB-	denotes	(
T26432	17889-17894	JJ	denotes	right
T26433	17894-17895	-RRB-	denotes	)
T26434	17896-17901	NN	denotes	E18.5
T26405	17902-17909	NNS	denotes	kidneys
T26435	17909-17910	.	denotes	.
T26436	17910-17944	sentence	denotes	The kidneys in (N) were bisected.
T26437	17911-17914	DT	denotes	The
T26438	17915-17922	NNS	denotes	kidneys
T26440	17923-17925	IN	denotes	in
T26441	17926-17927	-LRB-	denotes	(
T26442	17927-17928	NN	denotes	N
T26443	17928-17929	-RRB-	denotes	)
T26444	17930-17934	VBD	denotes	were
T26439	17935-17943	VBN	denotes	bisected
T26445	17943-17944	.	denotes	.
T26446	17944-18132	sentence	denotes	BAT-gal reporter expression was seen in the ureteric tree components of the cortex and medulla of the double heterozygotes but was almost completely lost in the double-homozygous mutants.
T26447	17945-17948	NN	denotes	BAT
T26449	17948-17949	HYPH	denotes	-
T26448	17949-17952	NN	denotes	gal
T26450	17953-17961	NN	denotes	reporter
T26451	17962-17972	NN	denotes	expression
T26453	17973-17976	VBD	denotes	was
T26452	17977-17981	VBN	denotes	seen
T26454	17982-17984	IN	denotes	in
T26455	17985-17988	DT	denotes	the
T26457	17989-17997	JJ	denotes	ureteric
T26458	17998-18002	NN	denotes	tree
T26456	18003-18013	NNS	denotes	components
T26459	18014-18016	IN	denotes	of
T26460	18017-18020	DT	denotes	the
T26461	18021-18027	NN	denotes	cortex
T26462	18028-18031	CC	denotes	and
T26463	18032-18039	NN	denotes	medulla
T26464	18040-18042	IN	denotes	of
T26465	18043-18046	DT	denotes	the
T26467	18047-18053	JJ	denotes	double
T26466	18054-18067	NNS	denotes	heterozygotes
T26468	18068-18071	CC	denotes	but
T26469	18072-18075	VBD	denotes	was
T26471	18076-18082	RB	denotes	almost
T26472	18083-18093	RB	denotes	completely
T26470	18094-18098	VBN	denotes	lost
T26473	18099-18101	IN	denotes	in
T26474	18102-18105	DT	denotes	the
T26476	18106-18112	JJ	denotes	double
T26478	18112-18113	HYPH	denotes	-
T26477	18113-18123	JJ	denotes	homozygous
T26475	18124-18131	NNS	denotes	mutants
T26479	18131-18132	.	denotes	.
T26480	18132-18235	sentence	denotes	(O) Pygo1+/-/Pygo2+/+ (left), Pygo1-/-/Pygo2+/- (middle), and Pygo1-/-/Pygo2-/- (right) E18.5 kidneys.
T26481	18133-18134	-LRB-	denotes	(
T26482	18134-18135	LS	denotes	O
T26484	18135-18136	-RRB-	denotes	)
T26485	18137-18142	NN	denotes	Pygo1
T26486	18142-18143	SYM	denotes	+
T26487	18143-18144	HYPH	denotes	/
T26488	18144-18145	SYM	denotes	-
T26489	18145-18146	HYPH	denotes	/
T26490	18146-18151	NN	denotes	Pygo2
T26491	18151-18152	SYM	denotes	+
T26492	18152-18153	HYPH	denotes	/
T26493	18153-18154	SYM	denotes	+
T26494	18155-18156	-LRB-	denotes	(
T26495	18156-18160	JJ	denotes	left
T26496	18160-18161	-RRB-	denotes	)
T26497	18161-18163	,	denotes	,
T26498	18163-18168	NN	denotes	Pygo1
T26499	18168-18169	SYM	denotes	-
T26500	18169-18170	HYPH	denotes	/
T26501	18170-18171	SYM	denotes	-
T26502	18171-18172	HYPH	denotes	/
T26503	18172-18177	NN	denotes	Pygo2
T26504	18177-18178	SYM	denotes	+
T26505	18178-18179	HYPH	denotes	/
T26506	18179-18180	SYM	denotes	-
T26507	18181-18182	-LRB-	denotes	(
T26508	18182-18188	JJ	denotes	middle
T26509	18188-18189	-RRB-	denotes	)
T26510	18189-18191	,	denotes	,
T26511	18191-18194	CC	denotes	and
T26512	18195-18200	NN	denotes	Pygo1
T26513	18200-18201	SYM	denotes	-
T26514	18201-18202	HYPH	denotes	/
T26515	18202-18203	SYM	denotes	-
T26516	18203-18204	HYPH	denotes	/
T26517	18204-18209	NN	denotes	Pygo2
T26518	18209-18210	SYM	denotes	-
T26519	18210-18211	HYPH	denotes	/
T26520	18211-18212	SYM	denotes	-
T26521	18213-18214	-LRB-	denotes	(
T26522	18214-18219	JJ	denotes	right
T26523	18219-18220	-RRB-	denotes	)
T26524	18221-18226	NN	denotes	E18.5
T26483	18227-18234	NNS	denotes	kidneys
T26525	18234-18235	.	denotes	.
T26526	18235-18416	sentence	denotes	Reporter activity was present in the ureteric compartments of the Pygo1+/-/Pygo2+/+ (left) and Pygo1-/-/Pygo2+/- (middle) kidneys, but lost in the Pygo1-/-/Pygo2-/- (right) kidney.
T26527	18236-18244	NN	denotes	Reporter
T26528	18245-18253	NN	denotes	activity
T26529	18254-18257	VBD	denotes	was
T26530	18258-18265	JJ	denotes	present
T26531	18266-18268	IN	denotes	in
T26532	18269-18272	DT	denotes	the
T26534	18273-18281	JJ	denotes	ureteric
T26533	18282-18294	NNS	denotes	compartments
T26535	18295-18297	IN	denotes	of
T26536	18298-18301	DT	denotes	the
T26538	18302-18307	NN	denotes	Pygo1
T26539	18307-18308	SYM	denotes	+
T26540	18308-18309	HYPH	denotes	/
T26541	18309-18310	SYM	denotes	-
T26542	18310-18311	HYPH	denotes	/
T26543	18311-18316	NN	denotes	Pygo2
T26544	18316-18317	SYM	denotes	+
T26545	18317-18318	HYPH	denotes	/
T26546	18318-18319	SYM	denotes	+
T26547	18320-18321	-LRB-	denotes	(
T26548	18321-18325	JJ	denotes	left
T26549	18325-18326	-RRB-	denotes	)
T26550	18327-18330	CC	denotes	and
T26551	18331-18336	NN	denotes	Pygo1
T26552	18336-18337	SYM	denotes	-
T26553	18337-18338	HYPH	denotes	/
T26554	18338-18339	SYM	denotes	-
T26555	18339-18340	HYPH	denotes	/
T26556	18340-18345	NN	denotes	Pygo2
T26557	18345-18346	SYM	denotes	+
T26558	18346-18347	HYPH	denotes	/
T26559	18347-18348	SYM	denotes	-
T26560	18349-18350	-LRB-	denotes	(
T26561	18350-18356	JJ	denotes	middle
T26562	18356-18357	-RRB-	denotes	)
T26537	18358-18365	NNS	denotes	kidneys
T26563	18365-18367	,	denotes	,
T26564	18367-18370	CC	denotes	but
T26565	18371-18375	JJ	denotes	lost
T26566	18376-18378	IN	denotes	in
T26567	18379-18382	DT	denotes	the
T26569	18383-18388	NN	denotes	Pygo1
T26570	18388-18389	SYM	denotes	-
T26571	18389-18390	HYPH	denotes	/
T26572	18390-18391	SYM	denotes	-
T26573	18391-18392	HYPH	denotes	/
T26574	18392-18397	NN	denotes	Pygo2
T26575	18397-18398	SYM	denotes	-
T26576	18398-18399	HYPH	denotes	/
T26577	18399-18400	SYM	denotes	-
T26578	18401-18402	-LRB-	denotes	(
T26579	18402-18407	JJ	denotes	right
T26580	18407-18408	-RRB-	denotes	)
T26568	18409-18415	NN	denotes	kidney
T26581	18415-18416	.	denotes	.
T26582	18416-18517	sentence	denotes	Original magnification: (A-C) × 32, (D-F) × 63, (G-I) × 40, (J-L) × 50, (M, N) × 10, and (O) × 12.5.
T26583	18417-18425	JJ	denotes	Original
T26584	18426-18439	NN	denotes	magnification
T26586	18439-18441	:	denotes	:
T26587	18441-18442	-LRB-	denotes	(
T26588	18442-18443	NN	denotes	A
T26589	18443-18444	SYM	denotes	-
T26590	18444-18445	NN	denotes	C
T26591	18445-18446	-RRB-	denotes	)
T26592	18447-18448	SYM	denotes	×
T26585	18449-18451	CD	denotes	32
T26593	18451-18453	,	denotes	,
T26594	18453-18454	-LRB-	denotes	(
T26595	18454-18455	NN	denotes	D
T26597	18455-18456	SYM	denotes	-
T26598	18456-18457	NN	denotes	F
T26599	18457-18458	-RRB-	denotes	)
T26600	18459-18460	SYM	denotes	×
T26596	18461-18463	CD	denotes	63
T26601	18463-18465	,	denotes	,
T26602	18465-18466	-LRB-	denotes	(
T26603	18466-18467	NN	denotes	G
T26605	18467-18468	SYM	denotes	-
T26606	18468-18469	NN	denotes	I
T26607	18469-18470	-RRB-	denotes	)
T26608	18471-18472	SYM	denotes	×
T26604	18473-18475	CD	denotes	40
T26609	18475-18477	,	denotes	,
T26610	18477-18478	-LRB-	denotes	(
T26611	18478-18479	NN	denotes	J
T26613	18479-18480	SYM	denotes	-
T26614	18480-18481	NN	denotes	L
T26615	18481-18482	-RRB-	denotes	)
T26616	18483-18484	SYM	denotes	×
T26612	18485-18487	CD	denotes	50
T26617	18487-18489	,	denotes	,
T26618	18489-18490	-LRB-	denotes	(
T26620	18490-18491	NN	denotes	M
T26621	18491-18493	,	denotes	,
T26619	18493-18494	NN	denotes	N
T26623	18494-18495	-RRB-	denotes	)
T26624	18496-18497	SYM	denotes	×
T26622	18498-18500	CD	denotes	10
T26625	18500-18502	,	denotes	,
T26626	18502-18505	CC	denotes	and
T26627	18506-18507	-LRB-	denotes	(
T26628	18507-18508	NN	denotes	O
T26630	18508-18509	-RRB-	denotes	)
T26631	18510-18511	SYM	denotes	×
T26629	18512-18516	CD	denotes	12.5
T26632	18516-18517	.	denotes	.
T9595	18518-18519	NN	denotes	A
T9594	18518-18521	sentence	denotes	At
T9596	18519-18520	NN	denotes	t
T9598	18521-18523	NN	denotes	E1
T9597	18521-18528	sentence	denotes	E13.5,
T9599	18523-18526	NN	denotes	3.5
T9600	18526-18528	,	denotes	,
T9602	18528-18529	JJ	denotes	t
T9601	18528-18530	sentence	denotes	th
T9603	18529-18530	JJ	denotes	h
T9605	18530-18531	VB	denotes	e
T9604	18530-18539	sentence	denotes	e Pygo2 g
T9606	18532-18537	NN	denotes	Pygo2
T9607	18538-18539	JJ	denotes	g
T9609	18539-18540	NN	denotes	e
T9608	18539-18544	sentence	denotes	ene a
T9610	18540-18541	IN	denotes	n
T9611	18541-18542	DT	denotes	e
T9612	18543-18544	JJ	denotes	a
T9614	18544-18545	NN	denotes	p
T9613	18544-18554	sentence	denotes	ppeared to
T9615	18545-18547	NN	denotes	pe
T9616	18547-18548	CC	denotes	a
T9617	18548-18551	VBN	denotes	red
T9618	18552-18553	DT	denotes	t
T9619	18553-18554	NN	denotes	o
T9620	18554-18555	sentence	denotes
T9622	18555-18556	NN	denotes	p
T9621	18555-18558	sentence	denotes	pla
T9623	18556-18557	VBG	denotes	l
T9624	18557-18558	CC	denotes	a
T9626	18558-18559	NN	denotes	y
T9625	18558-18564	sentence	denotes	y a ma
T9627	18559-18560	,	denotes
T9628	18560-18561	JJ	denotes	a
T9629	18562-18564	NN	denotes	ma
T9632	18564-18567	JJ	denotes	jor
T9630	18564-18690	sentence	denotes	jor role, and the Pygo1 gene a minor role, in canonical Wnt signaling in the ureteric tree, as measured by BAT-gal expression.
T9631	18568-18572	NN	denotes	role
T9633	18572-18574	,	denotes	,
T9634	18574-18577	CC	denotes	and
T9635	18578-18581	DT	denotes	the
T9637	18582-18587	NN	denotes	Pygo1
T9636	18588-18592	NN	denotes	gene
T9639	18593-18594	DT	denotes	a
T9640	18595-18600	JJ	denotes	minor
T9638	18601-18605	NN	denotes	role
T9641	18605-18607	,	denotes	,
T9642	18607-18609	IN	denotes	in
T9643	18610-18619	JJ	denotes	canonical
T9645	18620-18623	NN	denotes	Wnt
T9644	18624-18633	NN	denotes	signaling
T9646	18634-18636	IN	denotes	in
T9647	18637-18640	DT	denotes	the
T9649	18641-18649	JJ	denotes	ureteric
T9648	18650-18654	NN	denotes	tree
T9650	18654-18656	,	denotes	,
T9651	18656-18658	IN	denotes	as
T9652	18659-18667	VBN	denotes	measured
T9653	18668-18670	IN	denotes	by
T9654	18671-18674	NN	denotes	BAT
T9656	18674-18675	HYPH	denotes	-
T9655	18675-18678	NN	denotes	gal
T9657	18679-18689	NN	denotes	expression
T9658	18689-18690	.	denotes	.
T9659	18690-18940	sentence	denotes	A negative control kidney, without the BAT-gal transgene, showed minimal background X-gal staining (Figure 6D), whereas a BAT-gal transgenic kidney with at least one wild-type Pygo2 gene showed strong X-gal staining in the ureteric tree (Figure 6E).
T9660	18691-18692	DT	denotes	A
T9662	18693-18701	JJ	denotes	negative
T9663	18702-18709	NN	denotes	control
T9661	18710-18716	NN	denotes	kidney
T9665	18716-18718	,	denotes	,
T9666	18718-18725	IN	denotes	without
T9667	18726-18729	DT	denotes	the
T9669	18730-18733	NN	denotes	BAT
T9671	18733-18734	HYPH	denotes	-
T9670	18734-18737	NN	denotes	gal
T9668	18738-18747	NN	denotes	transgene
T9672	18747-18749	,	denotes	,
T9664	18749-18755	VBD	denotes	showed
T9673	18756-18763	JJ	denotes	minimal
T9674	18764-18774	NN	denotes	background
T9676	18775-18776	NN	denotes	X
T9678	18776-18777	HYPH	denotes	-
T9677	18777-18780	NN	denotes	gal
T9675	18781-18789	NN	denotes	staining
T9679	18790-18791	-LRB-	denotes	(
T9681	18791-18797	NN	denotes	Figure
T9680	18798-18800	NN	denotes	6D
T9682	18800-18801	-RRB-	denotes	)
T9683	18801-18803	,	denotes	,
T9684	18803-18810	IN	denotes	whereas
T9686	18811-18812	DT	denotes	a
T9688	18813-18816	NN	denotes	BAT
T9690	18816-18817	HYPH	denotes	-
T9689	18817-18820	NN	denotes	gal
T9691	18821-18831	JJ	denotes	transgenic
T9687	18832-18838	NN	denotes	kidney
T9692	18839-18843	IN	denotes	with
T9693	18844-18846	RB	denotes	at
T9695	18847-18852	RBS	denotes	least
T9694	18853-18856	CD	denotes	one
T9697	18857-18861	JJ	denotes	wild
T9699	18861-18862	HYPH	denotes	-
T9698	18862-18866	NN	denotes	type
T9700	18867-18872	NN	denotes	Pygo2
T9696	18873-18877	NN	denotes	gene
T9685	18878-18884	VBD	denotes	showed
T9701	18885-18891	JJ	denotes	strong
T9703	18892-18893	NN	denotes	X
T9705	18893-18894	HYPH	denotes	-
T9704	18894-18897	NN	denotes	gal
T9702	18898-18906	NN	denotes	staining
T9706	18907-18909	IN	denotes	in
T9707	18910-18913	DT	denotes	the
T9709	18914-18922	JJ	denotes	ureteric
T9708	18923-18927	NN	denotes	tree
T9710	18928-18929	-LRB-	denotes	(
T9712	18929-18935	NN	denotes	Figure
T9711	18936-18938	NN	denotes	6E
T9713	18938-18939	-RRB-	denotes	)
T9714	18939-18940	.	denotes	.
T9715	18940-19093	sentence	denotes	In contrast, Pygo1+/-/Pygo2-/- E13.5 kidneys showed very weak reporter expression (Figure 6F), suggesting a significant loss of canonical Wnt signaling.
T9716	18941-18943	IN	denotes	In
T9718	18944-18952	NN	denotes	contrast
T9719	18952-18954	,	denotes	,
T9720	18954-18959	NN	denotes	Pygo1
T9722	18959-18960	SYM	denotes	+
T9723	18960-18961	HYPH	denotes	/
T9724	18961-18962	SYM	denotes	-
T9725	18962-18963	HYPH	denotes	/
T9726	18963-18968	NN	denotes	Pygo2
T9727	18968-18969	SYM	denotes	-
T9728	18969-18970	HYPH	denotes	/
T9729	18970-18971	SYM	denotes	-
T9730	18972-18977	NN	denotes	E13.5
T9721	18978-18985	NNS	denotes	kidneys
T9717	18986-18992	VBD	denotes	showed
T9731	18993-18997	RB	denotes	very
T9732	18998-19002	JJ	denotes	weak
T9734	19003-19011	NN	denotes	reporter
T9733	19012-19022	NN	denotes	expression
T9735	19023-19024	-LRB-	denotes	(
T9737	19024-19030	NN	denotes	Figure
T9736	19031-19033	NN	denotes	6F
T9738	19033-19034	-RRB-	denotes	)
T9739	19034-19036	,	denotes	,
T9740	19036-19046	VBG	denotes	suggesting
T9741	19047-19048	DT	denotes	a
T9743	19049-19060	JJ	denotes	significant
T9742	19061-19065	NN	denotes	loss
T9744	19066-19068	IN	denotes	of
T9745	19069-19078	JJ	denotes	canonical
T9747	19079-19082	NN	denotes	Wnt
T9746	19083-19092	NN	denotes	signaling
T9748	19092-19093	.	denotes	.
T9749	19093-19200	sentence	denotes	Homozygous loss of the Pygo1 gene alone, however, had a small effect on reporter expression (Figure 6G–H).
T9750	19094-19104	JJ	denotes	Homozygous
T9751	19105-19109	NN	denotes	loss
T9753	19110-19112	IN	denotes	of
T9754	19113-19116	DT	denotes	the
T9756	19117-19122	NN	denotes	Pygo1
T9755	19123-19127	NN	denotes	gene
T9757	19128-19133	RB	denotes	alone
T9758	19133-19135	,	denotes	,
T9759	19135-19142	RB	denotes	however
T9760	19142-19144	,	denotes	,
T9752	19144-19147	VBD	denotes	had
T9761	19148-19149	DT	denotes	a
T9763	19150-19155	JJ	denotes	small
T9762	19156-19162	NN	denotes	effect
T9764	19163-19165	IN	denotes	on
T9765	19166-19174	NN	denotes	reporter
T9766	19175-19185	NN	denotes	expression
T9767	19186-19187	-LRB-	denotes	(
T9769	19187-19193	NN	denotes	Figure
T9768	19194-19196	NN	denotes	6G
T9770	19196-19197	SYM	denotes	–
T9771	19197-19198	NN	denotes	H
T9772	19198-19199	-RRB-	denotes	)
T9773	19199-19200	.	denotes	.
T9774	19200-19348	sentence	denotes	Homozygous mutation of both the Pygo1 and Pygo2 genes gave a more dramatic reduction of BAT-gal expression than loss of Pygo2 alone (Figure 6F, L).
T9775	19201-19211	JJ	denotes	Homozygous
T9776	19212-19220	NN	denotes	mutation
T9778	19221-19223	IN	denotes	of
T9779	19224-19228	CC	denotes	both
T9781	19229-19232	DT	denotes	the
T9782	19233-19238	NN	denotes	Pygo1
T9783	19239-19242	CC	denotes	and
T9784	19243-19248	NN	denotes	Pygo2
T9780	19249-19254	NNS	denotes	genes
T9777	19255-19259	VBD	denotes	gave
T9785	19260-19261	DT	denotes	a
T9787	19262-19266	RBR	denotes	more
T9788	19267-19275	JJ	denotes	dramatic
T9786	19276-19285	NN	denotes	reduction
T9789	19286-19288	IN	denotes	of
T9790	19289-19292	NN	denotes	BAT
T9792	19292-19293	HYPH	denotes	-
T9791	19293-19296	NN	denotes	gal
T9793	19297-19307	NN	denotes	expression
T9794	19308-19312	IN	denotes	than
T9795	19313-19317	NN	denotes	loss
T9796	19318-19320	IN	denotes	of
T9797	19321-19326	NN	denotes	Pygo2
T9798	19327-19332	RB	denotes	alone
T9799	19333-19334	-LRB-	denotes	(
T9801	19334-19340	NN	denotes	Figure
T9802	19341-19343	NN	denotes	6F
T9803	19343-19345	,	denotes	,
T9800	19345-19346	NN	denotes	L
T9804	19346-19347	-RRB-	denotes	)
T9805	19347-19348	.	denotes	.
T9806	19348-19467	sentence	denotes	The Pygo1 and Pygo2 genes were also required for BAT-gal reporter expression in the paramesonephric (Mullerian) ducts.
T9807	19349-19352	DT	denotes	The
T9809	19353-19358	NN	denotes	Pygo1
T9810	19359-19362	CC	denotes	and
T9811	19363-19368	NN	denotes	Pygo2
T9808	19369-19374	NNS	denotes	genes
T9813	19375-19379	VBD	denotes	were
T9814	19380-19384	RB	denotes	also
T9812	19385-19393	VBN	denotes	required
T9815	19394-19397	IN	denotes	for
T9816	19398-19401	NN	denotes	BAT
T9818	19401-19402	HYPH	denotes	-
T9817	19402-19405	NN	denotes	gal
T9819	19406-19414	NN	denotes	reporter
T9820	19415-19425	NN	denotes	expression
T9821	19426-19428	IN	denotes	in
T9822	19429-19432	DT	denotes	the
T9824	19433-19448	JJ	denotes	paramesonephric
T9825	19449-19450	-LRB-	denotes	(
T9826	19450-19459	JJ	denotes	Mullerian
T9827	19459-19460	-RRB-	denotes	)
T9823	19461-19466	NNS	denotes	ducts
T9828	19466-19467	.	denotes	.
T9829	19467-19772	sentence	denotes	In Pygo2-/- mice, there was a significant loss of reporter expression (data not shown), and double-homozygous mutants showed loss of X-gal staining in the paramesonephric ducts (Figure 6K), whereas Pygo1-/-/Pygo2+/- and Pygo1+/-/Pygo2+/- mice showed normal levels of BAT-gal expression (Figure 6G, H, J).
T9830	19468-19470	IN	denotes	In
T9832	19471-19476	NN	denotes	Pygo2
T9834	19476-19477	SYM	denotes	-
T9835	19477-19478	HYPH	denotes	/
T9836	19478-19479	SYM	denotes	-
T9833	19480-19484	NNS	denotes	mice
T9837	19484-19486	,	denotes	,
T9838	19486-19491	EX	denotes	there
T9831	19492-19495	VBD	denotes	was
T9839	19496-19497	DT	denotes	a
T9841	19498-19509	JJ	denotes	significant
T9840	19510-19514	NN	denotes	loss
T9842	19515-19517	IN	denotes	of
T9843	19518-19526	NN	denotes	reporter
T9844	19527-19537	NN	denotes	expression
T9845	19538-19539	-LRB-	denotes	(
T9847	19539-19543	NNS	denotes	data
T9848	19544-19547	RB	denotes	not
T9846	19548-19553	VBN	denotes	shown
T9849	19553-19554	-RRB-	denotes	)
T9850	19554-19556	,	denotes	,
T9851	19556-19559	CC	denotes	and
T9852	19560-19566	JJ	denotes	double
T9854	19566-19567	HYPH	denotes	-
T9853	19567-19577	JJ	denotes	homozygous
T9855	19578-19585	NNS	denotes	mutants
T9856	19586-19592	VBD	denotes	showed
T9857	19593-19597	NN	denotes	loss
T9858	19598-19600	IN	denotes	of
T9859	19601-19602	NN	denotes	X
T9861	19602-19603	HYPH	denotes	-
T9860	19603-19606	NN	denotes	gal
T9862	19607-19615	NN	denotes	staining
T9863	19616-19618	IN	denotes	in
T9864	19619-19622	DT	denotes	the
T9866	19623-19638	JJ	denotes	paramesonephric
T9865	19639-19644	NNS	denotes	ducts
T9867	19645-19646	-LRB-	denotes	(
T9869	19646-19652	NN	denotes	Figure
T9868	19653-19655	NN	denotes	6K
T9870	19655-19656	-RRB-	denotes	)
T9871	19656-19658	,	denotes	,
T9872	19658-19665	IN	denotes	whereas
T9874	19666-19671	NN	denotes	Pygo1
T9876	19671-19672	SYM	denotes	-
T9877	19672-19673	HYPH	denotes	/
T9878	19673-19674	SYM	denotes	-
T9879	19674-19675	HYPH	denotes	/
T9880	19675-19680	NN	denotes	Pygo2
T9881	19680-19681	SYM	denotes	+
T9882	19681-19682	HYPH	denotes	/
T9883	19682-19683	SYM	denotes	-
T9884	19684-19687	CC	denotes	and
T9885	19688-19693	NN	denotes	Pygo1
T9886	19693-19694	SYM	denotes	+
T9887	19694-19695	HYPH	denotes	/
T9888	19695-19696	SYM	denotes	-
T9889	19696-19697	HYPH	denotes	/
T9890	19697-19702	NN	denotes	Pygo2
T9891	19702-19703	SYM	denotes	+
T9892	19703-19704	HYPH	denotes	/
T9893	19704-19705	SYM	denotes	-
T9875	19706-19710	NNS	denotes	mice
T9873	19711-19717	VBD	denotes	showed
T9894	19718-19724	JJ	denotes	normal
T9895	19725-19731	NNS	denotes	levels
T9896	19732-19734	IN	denotes	of
T9897	19735-19738	NN	denotes	BAT
T9899	19738-19739	HYPH	denotes	-
T9898	19739-19742	NN	denotes	gal
T9900	19743-19753	NN	denotes	expression
T9901	19754-19755	-LRB-	denotes	(
T9903	19755-19761	NN	denotes	Figure
T9904	19762-19764	NN	denotes	6G
T9905	19764-19766	,	denotes	,
T9906	19766-19767	NN	denotes	H
T9907	19767-19769	,	denotes	,
T9902	19769-19770	NN	denotes	J
T9908	19770-19771	-RRB-	denotes	)
T9909	19771-19772	.	denotes	.
T9910	19772-20014	sentence	denotes	BAT-gal reporter analysis of the Pygo mutants at a later developmental stage, E18.5, also identified a significant decrease in canonical Wnt signaling in the cortical ureteric branches and renal pelvis of the developing kidney (Figure 6M–O).
T9911	19773-19776	NN	denotes	BAT
T9913	19776-19777	HYPH	denotes	-
T9912	19777-19780	NN	denotes	gal
T9914	19781-19789	NN	denotes	reporter
T9915	19790-19798	NN	denotes	analysis
T9917	19799-19801	IN	denotes	of
T9918	19802-19805	DT	denotes	the
T9920	19806-19810	NN	denotes	Pygo
T9919	19811-19818	NNS	denotes	mutants
T9921	19819-19821	IN	denotes	at
T9922	19822-19823	DT	denotes	a
T9924	19824-19829	RB	denotes	later
T9925	19830-19843	JJ	denotes	developmental
T9923	19844-19849	NN	denotes	stage
T9926	19849-19851	,	denotes	,
T9927	19851-19856	NN	denotes	E18.5
T9928	19856-19858	,	denotes	,
T9929	19858-19862	RB	denotes	also
T9916	19863-19873	VBD	denotes	identified
T9930	19874-19875	DT	denotes	a
T9932	19876-19887	JJ	denotes	significant
T9931	19888-19896	NN	denotes	decrease
T9933	19897-19899	IN	denotes	in
T9934	19900-19909	JJ	denotes	canonical
T9936	19910-19913	NN	denotes	Wnt
T9935	19914-19923	NN	denotes	signaling
T9937	19924-19926	IN	denotes	in
T9938	19927-19930	DT	denotes	the
T9940	19931-19939	JJ	denotes	cortical
T9941	19940-19948	JJ	denotes	ureteric
T9939	19949-19957	NNS	denotes	branches
T9942	19958-19961	CC	denotes	and
T9943	19962-19967	JJ	denotes	renal
T9944	19968-19974	NN	denotes	pelvis
T9945	19975-19977	IN	denotes	of
T9946	19978-19981	DT	denotes	the
T9948	19982-19992	VBG	denotes	developing
T9947	19993-19999	NN	denotes	kidney
T9949	20000-20001	-LRB-	denotes	(
T9951	20001-20007	NN	denotes	Figure
T9950	20008-20010	NN	denotes	6M
T9952	20010-20011	SYM	denotes	–
T9953	20011-20012	NN	denotes	O
T9954	20012-20013	-RRB-	denotes	)
T9955	20013-20014	.	denotes	.
T9956	20014-20211	sentence	denotes	Cortical X-gal staining was seen in the ureteric branches of a Pygo1+/-/Pygo2+/- kidney (Figure 6M, left), but was completely absent in the cortex of a Pygo1+/-/Pygo2-/- kidney (Figure 6M, right).
T9957	20015-20023	JJ	denotes	Cortical
T9959	20024-20025	NN	denotes	X
T9961	20025-20026	HYPH	denotes	-
T9960	20026-20029	NN	denotes	gal
T9958	20030-20038	NN	denotes	staining
T9963	20039-20042	VBD	denotes	was
T9962	20043-20047	VBN	denotes	seen
T9964	20048-20050	IN	denotes	in
T9965	20051-20054	DT	denotes	the
T9967	20055-20063	JJ	denotes	ureteric
T9966	20064-20072	NNS	denotes	branches
T9968	20073-20075	IN	denotes	of
T9969	20076-20077	DT	denotes	a
T9971	20078-20083	NN	denotes	Pygo1
T9972	20083-20084	SYM	denotes	+
T9973	20084-20085	HYPH	denotes	/
T9974	20085-20086	SYM	denotes	-
T9975	20086-20087	HYPH	denotes	/
T9976	20087-20092	NN	denotes	Pygo2
T9977	20092-20093	SYM	denotes	+
T9978	20093-20094	HYPH	denotes	/
T9979	20094-20095	SYM	denotes	-
T9970	20096-20102	NN	denotes	kidney
T9980	20103-20104	-LRB-	denotes	(
T9982	20104-20110	NN	denotes	Figure
T9983	20111-20113	NN	denotes	6M
T9984	20113-20115	,	denotes	,
T9981	20115-20119	JJ	denotes	left
T9985	20119-20120	-RRB-	denotes	)
T9986	20120-20122	,	denotes	,
T9987	20122-20125	CC	denotes	but
T9988	20126-20129	VBD	denotes	was
T9989	20130-20140	RB	denotes	completely
T9990	20141-20147	JJ	denotes	absent
T9991	20148-20150	IN	denotes	in
T9992	20151-20154	DT	denotes	the
T9993	20155-20161	NN	denotes	cortex
T9994	20162-20164	IN	denotes	of
T9995	20165-20166	DT	denotes	a
T9997	20167-20172	NN	denotes	Pygo1
T9998	20172-20173	SYM	denotes	+
T9999	20173-20174	HYPH	denotes	/
T10000	20174-20175	SYM	denotes	-
T10001	20175-20176	HYPH	denotes	/
T10002	20176-20181	NN	denotes	Pygo2
T10003	20181-20182	SYM	denotes	-
T10004	20182-20183	HYPH	denotes	/
T10005	20183-20184	SYM	denotes	-
T9996	20185-20191	NN	denotes	kidney
T10006	20192-20193	-LRB-	denotes	(
T10008	20193-20199	NN	denotes	Figure
T10009	20200-20202	NN	denotes	6M
T10010	20202-20204	,	denotes	,
T10007	20204-20209	JJ	denotes	right
T10011	20209-20210	-RRB-	denotes	)
T10012	20210-20211	.	denotes	.
T10013	20211-20386	sentence	denotes	Bisection revealed a significant loss of X-gal staining cells in the collecting ducts and renal pelvis of the Pygo2 null kidney compared with control littermates (Figure 6N).
T10014	20212-20221	NN	denotes	Bisection
T10015	20222-20230	VBD	denotes	revealed
T10016	20231-20232	DT	denotes	a
T10018	20233-20244	JJ	denotes	significant
T10017	20245-20249	NN	denotes	loss
T10019	20250-20252	IN	denotes	of
T10020	20253-20254	NN	denotes	X
T10022	20254-20255	HYPH	denotes	-
T10021	20255-20258	NN	denotes	gal
T10023	20259-20267	VBG	denotes	staining
T10024	20268-20273	NNS	denotes	cells
T10025	20274-20276	IN	denotes	in
T10026	20277-20280	DT	denotes	the
T10028	20281-20291	VBG	denotes	collecting
T10027	20292-20297	NNS	denotes	ducts
T10029	20298-20301	CC	denotes	and
T10030	20302-20307	JJ	denotes	renal
T10031	20308-20314	NN	denotes	pelvis
T10032	20315-20317	IN	denotes	of
T10033	20318-20321	DT	denotes	the
T10035	20322-20327	NN	denotes	Pygo2
T10036	20328-20332	JJ	denotes	null
T10034	20333-20339	NN	denotes	kidney
T10037	20340-20348	VBN	denotes	compared
T10038	20349-20353	IN	denotes	with
T10039	20354-20361	NN	denotes	control
T10040	20362-20373	NNS	denotes	littermates
T10041	20374-20375	-LRB-	denotes	(
T10043	20375-20381	NN	denotes	Figure
T10042	20382-20384	NN	denotes	6N
T10044	20384-20385	-RRB-	denotes	)
T10045	20385-20386	.	denotes	.
T10046	20386-20661	sentence	denotes	Side by side comparison of Pygo1+/+/Pygo2+/- (Figure 6O, left), Pygo1-/-/Pygo2+/- (Figure 6O, middle), and Pygo1-/-/Pygo2-/- (Figure 6O, right) E18.5 kidneys suggested a significant role for the Pygo2 gene in canonical Wnt signaling in the ureteric tree and its derivatives.
T10047	20387-20391	NN	denotes	Side
T10049	20392-20394	IN	denotes	by
T10050	20395-20399	NN	denotes	side
T10048	20400-20410	NN	denotes	comparison
T10052	20411-20413	IN	denotes	of
T10053	20414-20419	NN	denotes	Pygo1
T10055	20419-20420	SYM	denotes	+
T10056	20420-20421	HYPH	denotes	/
T10057	20421-20422	SYM	denotes	+
T10058	20422-20423	HYPH	denotes	/
T10059	20423-20428	NN	denotes	Pygo2
T10060	20428-20429	SYM	denotes	+
T10061	20429-20430	HYPH	denotes	/
T10062	20430-20431	SYM	denotes	-
T10063	20432-20433	-LRB-	denotes	(
T10065	20433-20439	NN	denotes	Figure
T10066	20440-20442	NN	denotes	6O
T10067	20442-20444	,	denotes	,
T10064	20444-20448	JJ	denotes	left
T10068	20448-20449	-RRB-	denotes	)
T10069	20449-20451	,	denotes	,
T10070	20451-20456	NN	denotes	Pygo1
T10071	20456-20457	SYM	denotes	-
T10072	20457-20458	HYPH	denotes	/
T10073	20458-20459	SYM	denotes	-
T10074	20459-20460	HYPH	denotes	/
T10075	20460-20465	NN	denotes	Pygo2
T10076	20465-20466	SYM	denotes	+
T10077	20466-20467	HYPH	denotes	/
T10078	20467-20468	SYM	denotes	-
T10079	20469-20470	-LRB-	denotes	(
T10081	20470-20476	NN	denotes	Figure
T10082	20477-20479	NN	denotes	6O
T10083	20479-20481	,	denotes	,
T10080	20481-20487	JJ	denotes	middle
T10084	20487-20488	-RRB-	denotes	)
T10085	20488-20490	,	denotes	,
T10086	20490-20493	CC	denotes	and
T10087	20494-20499	NN	denotes	Pygo1
T10088	20499-20500	SYM	denotes	-
T10089	20500-20501	HYPH	denotes	/
T10090	20501-20502	SYM	denotes	-
T10091	20502-20503	HYPH	denotes	/
T10092	20503-20508	NN	denotes	Pygo2
T10093	20508-20509	SYM	denotes	-
T10094	20509-20510	HYPH	denotes	/
T10095	20510-20511	SYM	denotes	-
T10096	20512-20513	-LRB-	denotes	(
T10098	20513-20519	NN	denotes	Figure
T10099	20520-20522	NN	denotes	6O
T10100	20522-20524	,	denotes	,
T10097	20524-20529	JJ	denotes	right
T10101	20529-20530	-RRB-	denotes	)
T10102	20531-20536	NN	denotes	E18.5
T10054	20537-20544	NNS	denotes	kidneys
T10051	20545-20554	VBD	denotes	suggested
T10103	20555-20556	DT	denotes	a
T10105	20557-20568	JJ	denotes	significant
T10104	20569-20573	NN	denotes	role
T10106	20574-20577	IN	denotes	for
T10107	20578-20581	DT	denotes	the
T10109	20582-20587	NN	denotes	Pygo2
T10108	20588-20592	NN	denotes	gene
T10110	20593-20595	IN	denotes	in
T10111	20596-20605	JJ	denotes	canonical
T10113	20606-20609	NN	denotes	Wnt
T10112	20610-20619	NN	denotes	signaling
T10114	20620-20622	IN	denotes	in
T10115	20623-20626	DT	denotes	the
T10117	20627-20635	JJ	denotes	ureteric
T10116	20636-20640	NN	denotes	tree
T10118	20641-20644	CC	denotes	and
T10119	20645-20648	PRP$	denotes	its
T10120	20649-20660	NNS	denotes	derivatives
T10121	20660-20661	.	denotes	.
T10122	20661-20882	sentence	denotes	In order to validate and quantify the BAT-gal reporter expression changes in the Pygo2 null and Pygo1/Pygo2 nulls, we performed ELISA measurements of transgene specific β-galactosidase levels in E18.5 kidneys (Figure 7).
T10123	20662-20664	IN	denotes	In
T10125	20665-20670	NN	denotes	order
T10126	20671-20673	TO	denotes	to
T10127	20674-20682	VB	denotes	validate
T10128	20683-20686	CC	denotes	and
T10129	20687-20695	VB	denotes	quantify
T10130	20696-20699	DT	denotes	the
T10132	20700-20703	NN	denotes	BAT
T10134	20703-20704	HYPH	denotes	-
T10133	20704-20707	NN	denotes	gal
T10135	20708-20716	NN	denotes	reporter
T10136	20717-20727	NN	denotes	expression
T10131	20728-20735	NNS	denotes	changes
T10137	20736-20738	IN	denotes	in
T10138	20739-20742	DT	denotes	the
T10140	20743-20748	NN	denotes	Pygo2
T10139	20749-20753	NN	denotes	null
T10141	20754-20757	CC	denotes	and
T10142	20758-20763	NN	denotes	Pygo1
T10144	20763-20764	HYPH	denotes	/
T10143	20764-20769	NN	denotes	Pygo2
T10145	20770-20775	NNS	denotes	nulls
T10146	20775-20777	,	denotes	,
T10147	20777-20779	PRP	denotes	we
T10124	20780-20789	VBD	denotes	performed
T10148	20790-20795	NN	denotes	ELISA
T10149	20796-20808	NNS	denotes	measurements
T10150	20809-20811	IN	denotes	of
T10151	20812-20821	NN	denotes	transgene
T10152	20822-20830	JJ	denotes	specific
T10154	20831-20832	NN	denotes	β
T10156	20832-20833	HYPH	denotes	-
T10155	20833-20846	NN	denotes	galactosidase
T10153	20847-20853	NNS	denotes	levels
T10157	20854-20856	IN	denotes	in
T10158	20857-20862	NN	denotes	E18.5
T10159	20863-20870	NNS	denotes	kidneys
T10160	20871-20872	-LRB-	denotes	(
T10161	20872-20878	NN	denotes	Figure
T10162	20879-20880	CD	denotes	7
T10163	20880-20881	-RRB-	denotes	)
T10164	20881-20882	.	denotes	.
T10165	20882-21002	sentence	denotes	Loss of the Pygo2 gene (Pygo1+/-/Pygo2-/- and Pygo1-/-/Pygo2-/-) gave greater than 90% reduction in BAT-gal expression.
T10166	20883-20887	NN	denotes	Loss
T10168	20888-20890	IN	denotes	of
T10169	20891-20894	DT	denotes	the
T10171	20895-20900	NN	denotes	Pygo2
T10170	20901-20905	NN	denotes	gene
T10172	20906-20907	-LRB-	denotes	(
T10173	20907-20912	NN	denotes	Pygo1
T10174	20912-20913	SYM	denotes	+
T10175	20913-20914	HYPH	denotes	/
T10176	20914-20915	SYM	denotes	-
T10177	20915-20916	HYPH	denotes	/
T10178	20916-20921	NN	denotes	Pygo2
T10179	20921-20922	SYM	denotes	-
T10180	20922-20923	HYPH	denotes	/
T10181	20923-20924	SYM	denotes	-
T10182	20925-20928	CC	denotes	and
T10183	20929-20934	NN	denotes	Pygo1
T10184	20934-20935	SYM	denotes	-
T10185	20935-20936	HYPH	denotes	/
T10186	20936-20937	SYM	denotes	-
T10187	20937-20938	HYPH	denotes	/
T10188	20938-20943	NN	denotes	Pygo2
T10189	20943-20944	SYM	denotes	-
T10190	20944-20945	HYPH	denotes	/
T10191	20945-20946	SYM	denotes	-
T10192	20946-20947	-RRB-	denotes	)
T10167	20948-20952	VBD	denotes	gave
T10193	20953-20960	JJR	denotes	greater
T10195	20961-20965	IN	denotes	than
T10194	20966-20968	CD	denotes	90
T10196	20968-20969	NN	denotes	%
T10197	20970-20979	NN	denotes	reduction
T10198	20980-20982	IN	denotes	in
T10199	20983-20986	NN	denotes	BAT
T10201	20986-20987	HYPH	denotes	-
T10200	20987-20990	NN	denotes	gal
T10202	20991-21001	NN	denotes	expression
T10203	21001-21002	.	denotes	.
T10204	21002-21082	sentence	denotes	Loss of Pygo1 alone (Pygo1-/-/Pygo2+/+) did not result in a significant change.
T10205	21003-21007	NN	denotes	Loss
T10207	21008-21010	IN	denotes	of
T10208	21011-21016	NN	denotes	Pygo1
T10209	21017-21022	RB	denotes	alone
T10210	21023-21024	-LRB-	denotes	(
T10211	21024-21029	NN	denotes	Pygo1
T10212	21029-21030	SYM	denotes	-
T10213	21030-21031	HYPH	denotes	/
T10214	21031-21032	SYM	denotes	-
T10215	21032-21033	HYPH	denotes	/
T10216	21033-21038	NN	denotes	Pygo2
T10217	21038-21039	SYM	denotes	+
T10218	21039-21040	HYPH	denotes	/
T10219	21040-21041	SYM	denotes	+
T10220	21041-21042	-RRB-	denotes	)
T10221	21043-21046	VBD	denotes	did
T10222	21047-21050	RB	denotes	not
T10206	21051-21057	VB	denotes	result
T10223	21058-21060	IN	denotes	in
T10224	21061-21062	DT	denotes	a
T10226	21063-21074	JJ	denotes	significant
T10225	21075-21081	NN	denotes	change
T10227	21081-21082	.	denotes	.
T10228	21082-21246	sentence	denotes	Interestingly, however, the Pygo1-/-/Pygo2+/- showed only 50% of wild-type BAT-gal expression, suggesting a minor contribution by Pygo1 in canonical Wnt signaling.
T10229	21083-21096	RB	denotes	Interestingly
T10231	21096-21098	,	denotes	,
T10232	21098-21105	RB	denotes	however
T10233	21105-21107	,	denotes	,
T10234	21107-21110	DT	denotes	the
T10235	21111-21116	NN	denotes	Pygo1
T10236	21116-21117	SYM	denotes	-
T10237	21117-21118	HYPH	denotes	/
T10238	21118-21119	SYM	denotes	-
T10239	21119-21120	HYPH	denotes	/
T10240	21120-21125	NN	denotes	Pygo2
T10241	21125-21126	SYM	denotes	+
T10242	21126-21127	HYPH	denotes	/
T10243	21127-21128	SYM	denotes	-
T10230	21129-21135	VBD	denotes	showed
T10244	21136-21140	RB	denotes	only
T10245	21141-21143	CD	denotes	50
T10246	21143-21144	NN	denotes	%
T10247	21145-21147	IN	denotes	of
T10248	21148-21152	JJ	denotes	wild
T10250	21152-21153	HYPH	denotes	-
T10249	21153-21157	NN	denotes	type
T10252	21158-21161	NN	denotes	BAT
T10254	21161-21162	HYPH	denotes	-
T10253	21162-21165	NN	denotes	gal
T10251	21166-21176	NN	denotes	expression
T10255	21176-21178	,	denotes	,
T10256	21178-21188	VBG	denotes	suggesting
T10257	21189-21190	DT	denotes	a
T10259	21191-21196	JJ	denotes	minor
T10258	21197-21209	NN	denotes	contribution
T10260	21210-21212	IN	denotes	by
T10261	21213-21218	NN	denotes	Pygo1
T10262	21219-21221	IN	denotes	in
T10263	21222-21231	JJ	denotes	canonical
T10265	21232-21235	NN	denotes	Wnt
T10264	21236-21245	NN	denotes	signaling
T10266	21245-21246	.	denotes	.
T10267	21246-21522	sentence	denotes	Although BAT-gal expression was decreased in the E18.5 Pygo1-/-/Pygo2+/- kidney, confocal analysis of kidneys with this genotype revealed no dilated ureteric tips or significant changes in ureteric tip number per area compared with Pygo1-/-/Pygo2+/+ kidneys (data not shown).
T10268	21247-21255	IN	denotes	Although
T10270	21256-21259	NN	denotes	BAT
T10272	21259-21260	HYPH	denotes	-
T10271	21260-21263	NN	denotes	gal
T10273	21264-21274	NN	denotes	expression
T10274	21275-21278	VBD	denotes	was
T10269	21279-21288	VBN	denotes	decreased
T10276	21289-21291	IN	denotes	in
T10277	21292-21295	DT	denotes	the
T10279	21296-21301	NN	denotes	E18.5
T10280	21302-21307	NN	denotes	Pygo1
T10281	21307-21308	SYM	denotes	-
T10282	21308-21309	HYPH	denotes	/
T10283	21309-21310	SYM	denotes	-
T10284	21310-21311	HYPH	denotes	/
T10285	21311-21316	NN	denotes	Pygo2
T10286	21316-21317	SYM	denotes	+
T10287	21317-21318	HYPH	denotes	/
T10288	21318-21319	SYM	denotes	-
T10278	21320-21326	NN	denotes	kidney
T10289	21326-21328	,	denotes	,
T10290	21328-21336	JJ	denotes	confocal
T10291	21337-21345	NN	denotes	analysis
T10292	21346-21348	IN	denotes	of
T10293	21349-21356	NNS	denotes	kidneys
T10294	21357-21361	IN	denotes	with
T10295	21362-21366	DT	denotes	this
T10296	21367-21375	NN	denotes	genotype
T10275	21376-21384	VBD	denotes	revealed
T10297	21385-21387	DT	denotes	no
T10299	21388-21395	JJ	denotes	dilated
T10300	21396-21404	JJ	denotes	ureteric
T10298	21405-21409	NNS	denotes	tips
T10301	21410-21412	CC	denotes	or
T10302	21413-21424	JJ	denotes	significant
T10303	21425-21432	NNS	denotes	changes
T10304	21433-21435	IN	denotes	in
T10305	21436-21444	JJ	denotes	ureteric
T10306	21445-21448	NN	denotes	tip
T10307	21449-21455	NN	denotes	number
T10308	21456-21459	IN	denotes	per
T10309	21460-21464	NN	denotes	area
T10310	21465-21473	VBN	denotes	compared
T10311	21474-21478	IN	denotes	with
T10312	21479-21484	NN	denotes	Pygo1
T10314	21484-21485	SYM	denotes	-
T10315	21485-21486	HYPH	denotes	/
T10316	21486-21487	SYM	denotes	-
T10317	21487-21488	HYPH	denotes	/
T10318	21488-21493	NN	denotes	Pygo2
T10319	21493-21494	SYM	denotes	+
T10320	21494-21495	HYPH	denotes	/
T10321	21495-21496	SYM	denotes	+
T10313	21497-21504	NNS	denotes	kidneys
T10322	21505-21506	-LRB-	denotes	(
T10324	21506-21510	NNS	denotes	data
T10325	21511-21514	RB	denotes	not
T10323	21515-21520	VBN	denotes	shown
T10326	21520-21521	-RRB-	denotes	)
T10327	21521-21522	.	denotes	.
T10328	21522-21706	sentence	denotes	Collectively, these BAT-gal reporter results suggest a significant role for the Pygo1 and Pygo2 genes in canonical Wnt signaling during development of the ureteric tree of the kidney.
T10329	21523-21535	RB	denotes	Collectively
T10331	21535-21537	,	denotes	,
T10332	21537-21542	DT	denotes	these
T10334	21543-21546	NN	denotes	BAT
T10336	21546-21547	HYPH	denotes	-
T10335	21547-21550	NN	denotes	gal
T10337	21551-21559	NN	denotes	reporter
T10333	21560-21567	NNS	denotes	results
T10330	21568-21575	VBP	denotes	suggest
T10338	21576-21577	DT	denotes	a
T10340	21578-21589	JJ	denotes	significant
T10339	21590-21594	NN	denotes	role
T10341	21595-21598	IN	denotes	for
T10342	21599-21602	DT	denotes	the
T10344	21603-21608	NN	denotes	Pygo1
T10345	21609-21612	CC	denotes	and
T10346	21613-21618	NN	denotes	Pygo2
T10343	21619-21624	NNS	denotes	genes
T10347	21625-21627	IN	denotes	in
T10348	21628-21637	JJ	denotes	canonical
T10350	21638-21641	NN	denotes	Wnt
T10349	21642-21651	NN	denotes	signaling
T10351	21652-21658	IN	denotes	during
T10352	21659-21670	NN	denotes	development
T10353	21671-21673	IN	denotes	of
T10354	21674-21677	DT	denotes	the
T10356	21678-21686	JJ	denotes	ureteric
T10355	21687-21691	NN	denotes	tree
T10357	21692-21694	IN	denotes	of
T10358	21695-21698	DT	denotes	the
T10359	21699-21705	NN	denotes	kidney
T10360	21705-21706	.	denotes	.
T10361	21706-22518	sentence	denotes	Figure 7 Quantitative analysis of BAT-gal reporter expression in Pygo1/Pygo2 E18.5 kidney extracts. Transgene-specific beta-galactosidase was quantified by ELISA analysis. Pygo1+/-/Pygo2+/+, Pygo1+/-/Pygo2+/- and Pygo1+/-/Pygo2+/+ kidneys all showed similar levels of BAT-gal expression. In the Pygo2 hetereozygote, Pygo1 homozygous mutants, however, reporter expression was reduced by about 50%. In Pygo2 homozygous mutants BAT-gal expression was uniformly low, with or without wild-type Pygo1 alleles. Each experimental group included a sample size of at least four. Interestingly, however, even in wild-type mice the BAT-gal reporter showed no expression in the developing metanephric mesenchyme, or metanephric mesenchyme-derived structures, such as renal vesicles, S-shaped bodies, tubules, and glomeruli.
T26917	21717-21729	JJ	denotes	Quantitative
T26918	21730-21738	NN	denotes	analysis
T26919	21739-21741	IN	denotes	of
T26920	21742-21745	NN	denotes	BAT
T26922	21745-21746	HYPH	denotes	-
T26921	21746-21749	NN	denotes	gal
T26923	21750-21758	NN	denotes	reporter
T26924	21759-21769	NN	denotes	expression
T26925	21770-21772	IN	denotes	in
T26926	21773-21778	NN	denotes	Pygo1
T26928	21778-21779	HYPH	denotes	/
T26927	21779-21784	NN	denotes	Pygo2
T26930	21785-21790	NN	denotes	E18.5
T26931	21791-21797	NN	denotes	kidney
T26929	21798-21806	NNS	denotes	extracts
T26932	21806-21807	.	denotes	.
T26933	21807-21879	sentence	denotes	Transgene-specific beta-galactosidase was quantified by ELISA analysis.
T26934	21808-21817	NN	denotes	Transgene
T26936	21817-21818	HYPH	denotes	-
T26935	21818-21826	JJ	denotes	specific
T26938	21827-21831	NN	denotes	beta
T26939	21831-21832	HYPH	denotes	-
T26937	21832-21845	NN	denotes	galactosidase
T26941	21846-21849	VBD	denotes	was
T26940	21850-21860	VBN	denotes	quantified
T26942	21861-21863	IN	denotes	by
T26943	21864-21869	NNP	denotes	ELISA
T26944	21870-21878	NN	denotes	analysis
T26945	21878-21879	.	denotes	.
T26946	21879-21995	sentence	denotes	Pygo1+/-/Pygo2+/+, Pygo1+/-/Pygo2+/- and Pygo1+/-/Pygo2+/+ kidneys all showed similar levels of BAT-gal expression.
T26947	21880-21885	NN	denotes	Pygo1
T26949	21885-21886	SYM	denotes	+
T26950	21886-21887	HYPH	denotes	/
T26951	21887-21888	SYM	denotes	-
T26952	21888-21889	HYPH	denotes	/
T26953	21889-21894	NN	denotes	Pygo2
T26954	21894-21895	SYM	denotes	+
T26955	21895-21896	HYPH	denotes	/
T26956	21896-21897	SYM	denotes	+
T26957	21897-21899	,	denotes	,
T26958	21899-21904	NN	denotes	Pygo1
T26959	21904-21905	SYM	denotes	+
T26960	21905-21906	HYPH	denotes	/
T26961	21906-21907	SYM	denotes	-
T26962	21907-21908	HYPH	denotes	/
T26963	21908-21913	NN	denotes	Pygo2
T26964	21913-21914	SYM	denotes	+
T26965	21914-21915	HYPH	denotes	/
T26966	21915-21916	SYM	denotes	-
T26967	21917-21920	CC	denotes	and
T26968	21921-21926	NN	denotes	Pygo1
T26969	21926-21927	SYM	denotes	+
T26970	21927-21928	HYPH	denotes	/
T26971	21928-21929	SYM	denotes	-
T26972	21929-21930	HYPH	denotes	/
T26973	21930-21935	NN	denotes	Pygo2
T26974	21935-21936	SYM	denotes	+
T26975	21936-21937	HYPH	denotes	/
T26976	21937-21938	SYM	denotes	+
T26948	21939-21946	NNS	denotes	kidneys
T26978	21947-21950	RB	denotes	all
T26977	21951-21957	VBD	denotes	showed
T26979	21958-21965	JJ	denotes	similar
T26980	21966-21972	NNS	denotes	levels
T26981	21973-21975	IN	denotes	of
T26982	21976-21979	NN	denotes	BAT
T26984	21979-21980	HYPH	denotes	-
T26983	21980-21983	NN	denotes	gal
T26985	21984-21994	NN	denotes	expression
T26986	21994-21995	.	denotes	.
T26987	21995-22104	sentence	denotes	In the Pygo2 hetereozygote, Pygo1 homozygous mutants, however, reporter expression was reduced by about 50%.
T26988	21996-21998	IN	denotes	In
T26990	21999-22002	DT	denotes	the
T26992	22003-22008	NN	denotes	Pygo2
T26993	22009-22022	NN	denotes	hetereozygote
T26994	22022-22024	,	denotes	,
T26995	22024-22029	NN	denotes	Pygo1
T26996	22030-22040	JJ	denotes	homozygous
T26991	22041-22048	NNS	denotes	mutants
T26997	22048-22050	,	denotes	,
T26998	22050-22057	RB	denotes	however
T26999	22057-22059	,	denotes	,
T27000	22059-22067	NN	denotes	reporter
T27001	22068-22078	NN	denotes	expression
T27002	22079-22082	VBD	denotes	was
T26989	22083-22090	VBN	denotes	reduced
T27003	22091-22093	IN	denotes	by
T27004	22094-22099	IN	denotes	about
T27005	22100-22102	CD	denotes	50
T27006	22102-22103	NN	denotes	%
T27007	22103-22104	.	denotes	.
T27008	22104-22211	sentence	denotes	In Pygo2 homozygous mutants BAT-gal expression was uniformly low, with or without wild-type Pygo1 alleles.
T27009	22105-22107	IN	denotes	In
T27011	22108-22113	NN	denotes	Pygo2
T27012	22114-22124	JJ	denotes	homozygous
T27013	22125-22132	NNS	denotes	mutants
T27014	22133-22136	NN	denotes	BAT
T27016	22136-22137	HYPH	denotes	-
T27015	22137-22140	NN	denotes	gal
T27017	22141-22151	NN	denotes	expression
T27010	22152-22155	VBD	denotes	was
T27018	22156-22165	RB	denotes	uniformly
T27019	22166-22169	JJ	denotes	low
T27020	22169-22171	,	denotes	,
T27021	22171-22175	IN	denotes	with
T27022	22176-22178	CC	denotes	or
T27023	22179-22186	IN	denotes	without
T27024	22187-22191	JJ	denotes	wild
T27026	22191-22192	HYPH	denotes	-
T27025	22192-22196	NN	denotes	type
T27028	22197-22202	NN	denotes	Pygo1
T27027	22203-22210	NNS	denotes	alleles
T27029	22210-22211	.	denotes	.
T27030	22211-22276	sentence	denotes	Each experimental group included a sample size of at least four.
T27031	22212-22216	DT	denotes	Each
T27033	22217-22229	JJ	denotes	experimental
T27032	22230-22235	NN	denotes	group
T27034	22236-22244	VBD	denotes	included
T27035	22245-22246	DT	denotes	a
T27037	22247-22253	NN	denotes	sample
T27036	22254-22258	NN	denotes	size
T27038	22259-22261	IN	denotes	of
T27039	22262-22264	RB	denotes	at
T27041	22265-22270	RBS	denotes	least
T27040	22271-22275	CD	denotes	four
T27042	22275-22276	.	denotes	.
T10362	22277-22290	RB	denotes	Interestingly
T10364	22290-22292	,	denotes	,
T10365	22292-22299	RB	denotes	however
T10366	22299-22301	,	denotes	,
T10367	22301-22305	RB	denotes	even
T10368	22306-22308	IN	denotes	in
T10369	22309-22313	JJ	denotes	wild
T10371	22313-22314	HYPH	denotes	-
T10370	22314-22318	NN	denotes	type
T10372	22319-22323	NNS	denotes	mice
T10373	22324-22327	DT	denotes	the
T10375	22328-22331	NN	denotes	BAT
T10377	22331-22332	HYPH	denotes	-
T10376	22332-22335	NN	denotes	gal
T10374	22336-22344	NN	denotes	reporter
T10363	22345-22351	VBD	denotes	showed
T10378	22352-22354	DT	denotes	no
T10379	22355-22365	NN	denotes	expression
T10380	22366-22368	IN	denotes	in
T10381	22369-22372	DT	denotes	the
T10383	22373-22383	VBG	denotes	developing
T10384	22384-22395	JJ	denotes	metanephric
T10382	22396-22406	NN	denotes	mesenchyme
T10385	22406-22408	,	denotes	,
T10386	22408-22410	CC	denotes	or
T10387	22411-22422	JJ	denotes	metanephric
T10388	22423-22433	NN	denotes	mesenchyme
T10390	22433-22434	HYPH	denotes	-
T10389	22434-22441	VBN	denotes	derived
T10391	22442-22452	NNS	denotes	structures
T10392	22452-22454	,	denotes	,
T10393	22454-22458	JJ	denotes	such
T10394	22459-22461	IN	denotes	as
T10395	22462-22467	JJ	denotes	renal
T10396	22468-22476	NNS	denotes	vesicles
T10397	22476-22478	,	denotes	,
T10398	22478-22479	NN	denotes	S
T10400	22479-22480	HYPH	denotes	-
T10399	22480-22486	VBN	denotes	shaped
T10401	22487-22493	NNS	denotes	bodies
T10402	22493-22495	,	denotes	,
T10403	22495-22502	NNS	denotes	tubules
T10404	22502-22504	,	denotes	,
T10405	22504-22507	CC	denotes	and
T10406	22508-22517	NNS	denotes	glomeruli
T10407	22517-22518	.	denotes	.
T10408	22518-22660	sentence	denotes	This has been reported previously, and was interpreted to indicate the absence of canonical Wnt signaling in the metanephric mesenchyme [21].
T10409	22519-22523	DT	denotes	This
T10411	22524-22527	VBZ	denotes	has
T10412	22528-22532	VBN	denotes	been
T10410	22533-22541	VBN	denotes	reported
T10413	22542-22552	RB	denotes	previously
T10414	22552-22554	,	denotes	,
T10415	22554-22557	CC	denotes	and
T10416	22558-22561	VBD	denotes	was
T10417	22562-22573	VBN	denotes	interpreted
T10418	22574-22576	TO	denotes	to
T10419	22577-22585	VB	denotes	indicate
T10420	22586-22589	DT	denotes	the
T10421	22590-22597	NN	denotes	absence
T10422	22598-22600	IN	denotes	of
T10423	22601-22610	JJ	denotes	canonical
T10425	22611-22614	NN	denotes	Wnt
T10424	22615-22624	NN	denotes	signaling
T10426	22625-22627	IN	denotes	in
T10427	22628-22631	DT	denotes	the
T10429	22632-22643	JJ	denotes	metanephric
T10428	22644-22654	NN	denotes	mesenchyme
T10430	22655-22656	-LRB-	denotes	[
T10431	22656-22658	CD	denotes	21
T10432	22658-22659	-RRB-	denotes	]
T10433	22659-22660	.	denotes	.
T10434	22660-22778	sentence	denotes	Results from other studies, however, argue for the presence of canonical Wnt signaling in the metanephric mesenchyme.
T10435	22661-22668	NNS	denotes	Results
T10437	22669-22673	IN	denotes	from
T10438	22674-22679	JJ	denotes	other
T10439	22680-22687	NNS	denotes	studies
T10440	22687-22689	,	denotes	,
T10441	22689-22696	RB	denotes	however
T10442	22696-22698	,	denotes	,
T10436	22698-22703	VBP	denotes	argue
T10443	22704-22707	IN	denotes	for
T10444	22708-22711	DT	denotes	the
T10445	22712-22720	NN	denotes	presence
T10446	22721-22723	IN	denotes	of
T10447	22724-22733	JJ	denotes	canonical
T10449	22734-22737	NN	denotes	Wnt
T10448	22738-22747	NN	denotes	signaling
T10450	22748-22750	IN	denotes	in
T10451	22751-22754	DT	denotes	the
T10453	22755-22766	JJ	denotes	metanephric
T10452	22767-22777	NN	denotes	mesenchyme
T10454	22777-22778	.	denotes	.
T10455	22778-22957	sentence	denotes	For example, ureteric bud expression of Wnt1, thought to act through canonical Wnt signaling, can rescue Wnt9b mutants and induce nephrogenesis of the metanephric mesenchyme [2].
T10456	22779-22782	IN	denotes	For
T10458	22783-22790	NN	denotes	example
T10459	22790-22792	,	denotes	,
T10460	22792-22800	JJ	denotes	ureteric
T10461	22801-22804	NN	denotes	bud
T10462	22805-22815	NN	denotes	expression
T10463	22816-22818	IN	denotes	of
T10464	22819-22823	NN	denotes	Wnt1
T10465	22823-22825	,	denotes	,
T10466	22825-22832	VBN	denotes	thought
T10467	22833-22835	TO	denotes	to
T10468	22836-22839	VB	denotes	act
T10469	22840-22847	IN	denotes	through
T10470	22848-22857	JJ	denotes	canonical
T10472	22858-22861	NN	denotes	Wnt
T10471	22862-22871	NN	denotes	signaling
T10473	22871-22873	,	denotes	,
T10474	22873-22876	MD	denotes	can
T10457	22877-22883	VB	denotes	rescue
T10475	22884-22889	NN	denotes	Wnt9b
T10476	22890-22897	NNS	denotes	mutants
T10477	22898-22901	CC	denotes	and
T10478	22902-22908	VB	denotes	induce
T10479	22909-22922	NN	denotes	nephrogenesis
T10480	22923-22925	IN	denotes	of
T10481	22926-22929	DT	denotes	the
T10483	22930-22941	JJ	denotes	metanephric
T10482	22942-22952	NN	denotes	mesenchyme
T10484	22953-22954	-LRB-	denotes	[
T10485	22954-22955	CD	denotes	2
T10486	22955-22956	-RRB-	denotes	]
T10487	22956-22957	.	denotes	.
T10488	22957-23081	sentence	denotes	This suggests that the BAT-gal transgene might not accurately report canonical Wnt signaling in the metanephric mesenchyme.
T10489	22958-22962	DT	denotes	This
T10490	22963-22971	VBZ	denotes	suggests
T10491	22972-22976	IN	denotes	that
T10493	22977-22980	DT	denotes	the
T10495	22981-22984	NN	denotes	BAT
T10497	22984-22985	HYPH	denotes	-
T10496	22985-22988	NN	denotes	gal
T10494	22989-22998	NN	denotes	transgene
T10498	22999-23004	MD	denotes	might
T10499	23005-23008	RB	denotes	not
T10500	23009-23019	RB	denotes	accurately
T10492	23020-23026	VB	denotes	report
T10501	23027-23036	JJ	denotes	canonical
T10503	23037-23040	NN	denotes	Wnt
T10502	23041-23050	NN	denotes	signaling
T10504	23051-23053	IN	denotes	in
T10505	23054-23057	DT	denotes	the
T10507	23058-23069	JJ	denotes	metanephric
T10506	23070-23080	NN	denotes	mesenchyme
T10508	23080-23081	.	denotes	.
T10509	23081-23325	sentence	denotes	To address this question, we incubated E11.5 metanephric mesenchyme in lithium chloride (LiCl), which activates canonical Wnt signaling through inhibition of GSK3, and also functions as an inducer of nephrogenesis in kidney organ culture [22].
T10510	23082-23084	TO	denotes	To
T10511	23085-23092	VB	denotes	address
T10513	23093-23097	DT	denotes	this
T10514	23098-23106	NN	denotes	question
T10515	23106-23108	,	denotes	,
T10516	23108-23110	PRP	denotes	we
T10512	23111-23120	VBD	denotes	incubated
T10517	23121-23126	NN	denotes	E11.5
T10519	23127-23138	JJ	denotes	metanephric
T10518	23139-23149	NN	denotes	mesenchyme
T10520	23150-23152	IN	denotes	in
T10521	23153-23160	NN	denotes	lithium
T10522	23161-23169	NN	denotes	chloride
T10523	23170-23171	-LRB-	denotes	(
T10524	23171-23175	NN	denotes	LiCl
T10525	23175-23176	-RRB-	denotes	)
T10526	23176-23178	,	denotes	,
T10527	23178-23183	WDT	denotes	which
T10528	23184-23193	VBZ	denotes	activates
T10529	23194-23203	JJ	denotes	canonical
T10531	23204-23207	NN	denotes	Wnt
T10530	23208-23217	NN	denotes	signaling
T10532	23218-23225	IN	denotes	through
T10533	23226-23236	NN	denotes	inhibition
T10534	23237-23239	IN	denotes	of
T10535	23240-23244	NN	denotes	GSK3
T10536	23244-23246	,	denotes	,
T10537	23246-23249	CC	denotes	and
T10538	23250-23254	RB	denotes	also
T10539	23255-23264	VBZ	denotes	functions
T10540	23265-23267	IN	denotes	as
T10541	23268-23270	DT	denotes	an
T10542	23271-23278	NN	denotes	inducer
T10543	23279-23281	IN	denotes	of
T10544	23282-23295	NN	denotes	nephrogenesis
T10545	23296-23298	IN	denotes	in
T10546	23299-23305	NN	denotes	kidney
T10547	23306-23311	NN	denotes	organ
T10548	23312-23319	NN	denotes	culture
T10549	23320-23321	-LRB-	denotes	[
T10550	23321-23323	CD	denotes	22
T10551	23323-23324	-RRB-	denotes	]
T10552	23324-23325	.	denotes	.
T10553	23325-23616	sentence	denotes	We observed that LiCl-treated metanephric mesenchyme did undergo nephrogenesis, as expected, but failed to show BAT-gal expression (data not shown), suggesting that this transgene is not an accurate reporter of canonical Wnt signaling in the metanephric mesenchyme of the developing kidney.
T10554	23326-23328	PRP	denotes	We
T10555	23329-23337	VBD	denotes	observed
T10556	23338-23342	IN	denotes	that
T10558	23343-23347	NN	denotes	LiCl
T10560	23347-23348	HYPH	denotes	-
T10559	23348-23355	VBN	denotes	treated
T10562	23356-23367	JJ	denotes	metanephric
T10561	23368-23378	NN	denotes	mesenchyme
T10563	23379-23382	VBD	denotes	did
T10557	23383-23390	VB	denotes	undergo
T10564	23391-23404	NN	denotes	nephrogenesis
T10565	23404-23406	,	denotes	,
T10566	23406-23408	IN	denotes	as
T10567	23409-23417	VBN	denotes	expected
T10568	23417-23419	,	denotes	,
T10569	23419-23422	CC	denotes	but
T10570	23423-23429	VBD	denotes	failed
T10571	23430-23432	TO	denotes	to
T10572	23433-23437	VB	denotes	show
T10573	23438-23441	NN	denotes	BAT
T10575	23441-23442	HYPH	denotes	-
T10574	23442-23445	NN	denotes	gal
T10576	23446-23456	NN	denotes	expression
T10577	23457-23458	-LRB-	denotes	(
T10579	23458-23462	NNS	denotes	data
T10580	23463-23466	RB	denotes	not
T10578	23467-23472	VBN	denotes	shown
T10581	23472-23473	-RRB-	denotes	)
T10582	23473-23475	,	denotes	,
T10583	23475-23485	VBG	denotes	suggesting
T10584	23486-23490	IN	denotes	that
T10586	23491-23495	DT	denotes	this
T10587	23496-23505	NN	denotes	transgene
T10585	23506-23508	VBZ	denotes	is
T10588	23509-23512	RB	denotes	not
T10589	23513-23515	DT	denotes	an
T10591	23516-23524	JJ	denotes	accurate
T10590	23525-23533	NN	denotes	reporter
T10592	23534-23536	IN	denotes	of
T10593	23537-23546	JJ	denotes	canonical
T10595	23547-23550	NN	denotes	Wnt
T10594	23551-23560	NN	denotes	signaling
T10596	23561-23563	IN	denotes	in
T10597	23564-23567	DT	denotes	the
T10599	23568-23579	JJ	denotes	metanephric
T10598	23580-23590	NN	denotes	mesenchyme
T10600	23591-23593	IN	denotes	of
T10601	23594-23597	DT	denotes	the
T10603	23598-23608	VBG	denotes	developing
T10602	23609-23615	NN	denotes	kidney
T10604	23615-23616	.	denotes	.
T12039	23618-23628	NN	denotes	Microarray
T12040	23629-23637	NN	denotes	analysis
T12041	23638-23640	IN	denotes	of
T12042	23641-23646	NN	denotes	Pygo1
T12044	23646-23647	HYPH	denotes	/
T12043	23647-23652	NN	denotes	Pygo2
T12045	23653-23657	JJ	denotes	null
T12046	23658-23665	NNS	denotes	kidneys
T12047	23665-23836	sentence	denotes	To further examine possible disturbance of gene expression in the Pygo1/Pygo2 mutant kidneys, we used microarrays to perform a global analysis of gene expression changes.
T12048	23666-23668	TO	denotes	To
T12050	23669-23676	RB	denotes	further
T12049	23677-23684	VB	denotes	examine
T12052	23685-23693	JJ	denotes	possible
T12053	23694-23705	NN	denotes	disturbance
T12054	23706-23708	IN	denotes	of
T12055	23709-23713	NN	denotes	gene
T12056	23714-23724	NN	denotes	expression
T12057	23725-23727	IN	denotes	in
T12058	23728-23731	DT	denotes	the
T12060	23732-23737	NN	denotes	Pygo1
T12062	23737-23738	HYPH	denotes	/
T12061	23738-23743	NN	denotes	Pygo2
T12063	23744-23750	NN	denotes	mutant
T12059	23751-23758	NNS	denotes	kidneys
T12064	23758-23760	,	denotes	,
T12065	23760-23762	PRP	denotes	we
T12051	23763-23767	VBD	denotes	used
T12066	23768-23779	NNS	denotes	microarrays
T12067	23780-23782	TO	denotes	to
T12068	23783-23790	VB	denotes	perform
T12069	23791-23792	DT	denotes	a
T12071	23793-23799	JJ	denotes	global
T12070	23800-23808	NN	denotes	analysis
T12072	23809-23811	IN	denotes	of
T12073	23812-23816	NN	denotes	gene
T12074	23817-23827	NN	denotes	expression
T12075	23828-23835	NNS	denotes	changes
T12076	23835-23836	.	denotes	.
T12077	23836-24031	sentence	denotes	Whereas the BAT-gal transgene reporter monitors the response of one promoter to Wnt signaling, microarrays can be used to follow expression changes of all genes, including all known Wnt targets.
T12078	23837-23844	IN	denotes	Whereas
T12080	23845-23848	DT	denotes	the
T12082	23849-23852	NN	denotes	BAT
T12084	23852-23853	HYPH	denotes	-
T12083	23853-23856	NN	denotes	gal
T12085	23857-23866	NN	denotes	transgene
T12081	23867-23875	NN	denotes	reporter
T12079	23876-23884	VBZ	denotes	monitors
T12087	23885-23888	DT	denotes	the
T12088	23889-23897	NN	denotes	response
T12089	23898-23900	IN	denotes	of
T12090	23901-23904	CD	denotes	one
T12091	23905-23913	NN	denotes	promoter
T12092	23914-23916	IN	denotes	to
T12093	23917-23920	NN	denotes	Wnt
T12094	23921-23930	NN	denotes	signaling
T12095	23930-23932	,	denotes	,
T12096	23932-23943	NNS	denotes	microarrays
T12097	23944-23947	MD	denotes	can
T12098	23948-23950	VB	denotes	be
T12086	23951-23955	VBN	denotes	used
T12099	23956-23958	TO	denotes	to
T12100	23959-23965	VB	denotes	follow
T12101	23966-23976	NN	denotes	expression
T12102	23977-23984	NNS	denotes	changes
T12103	23985-23987	IN	denotes	of
T12104	23988-23991	DT	denotes	all
T12105	23992-23997	NNS	denotes	genes
T12106	23997-23999	,	denotes	,
T12107	23999-24008	VBG	denotes	including
T12108	24009-24012	DT	denotes	all
T12110	24013-24018	VBN	denotes	known
T12111	24019-24022	NN	denotes	Wnt
T12109	24023-24030	NNS	denotes	targets
T12112	24030-24031	.	denotes	.
T12113	24031-24117	sentence	denotes	E18.5 wild-type and Pygo1-/-/Pygo2-/- kidneys were examined in biological triplicate.
T12114	24032-24037	NN	denotes	E18.5
T12116	24038-24042	JJ	denotes	wild
T12118	24042-24043	HYPH	denotes	-
T12117	24043-24047	NN	denotes	type
T12119	24048-24051	CC	denotes	and
T12120	24052-24057	NN	denotes	Pygo1
T12121	24057-24058	SYM	denotes	-
T12122	24058-24059	HYPH	denotes	/
T12123	24059-24060	SYM	denotes	-
T12124	24060-24061	HYPH	denotes	/
T12125	24061-24066	NN	denotes	Pygo2
T12126	24066-24067	SYM	denotes	-
T12127	24067-24068	HYPH	denotes	/
T12128	24068-24069	SYM	denotes	-
T12115	24070-24077	NNS	denotes	kidneys
T12130	24078-24082	VBD	denotes	were
T12129	24083-24091	VBN	denotes	examined
T12131	24092-24094	IN	denotes	in
T12132	24095-24105	JJ	denotes	biological
T12133	24106-24116	NN	denotes	triplicate
T12134	24116-24117	.	denotes	.
T12135	24117-24294	sentence	denotes	In total, 45 genes were identified as significantly changed, using a relatively low stringency screen of the data, with a p-value cutoff of <0.05, and fold change >2 (Table 3).
T12136	24118-24120	IN	denotes	In
T12138	24121-24126	JJ	denotes	total
T12139	24126-24128	,	denotes	,
T12140	24128-24130	CD	denotes	45
T12141	24131-24136	NNS	denotes	genes
T12142	24137-24141	VBD	denotes	were
T12137	24142-24152	VBN	denotes	identified
T12143	24153-24155	IN	denotes	as
T12144	24156-24169	RB	denotes	significantly
T12145	24170-24177	VBN	denotes	changed
T12146	24177-24179	,	denotes	,
T12147	24179-24184	VBG	denotes	using
T12148	24185-24186	DT	denotes	a
T12150	24187-24197	RB	denotes	relatively
T12151	24198-24201	JJ	denotes	low
T12152	24202-24212	NN	denotes	stringency
T12149	24213-24219	NN	denotes	screen
T12153	24220-24222	IN	denotes	of
T12154	24223-24226	DT	denotes	the
T12155	24227-24231	NNS	denotes	data
T12156	24231-24233	,	denotes	,
T12157	24233-24237	IN	denotes	with
T12158	24238-24239	DT	denotes	a
T12160	24240-24241	NN	denotes	p
T12162	24241-24242	HYPH	denotes	-
T12161	24242-24247	NN	denotes	value
T12159	24248-24254	NN	denotes	cutoff
T12163	24255-24257	IN	denotes	of
T12164	24258-24259	SYM	denotes	<
T12165	24259-24263	CD	denotes	0.05
T12166	24263-24265	,	denotes	,
T12167	24265-24268	CC	denotes	and
T12168	24269-24273	NN	denotes	fold
T12169	24274-24280	NN	denotes	change
T12170	24281-24282	SYM	denotes	>
T12171	24282-24283	CD	denotes	2
T12172	24284-24285	-LRB-	denotes	(
T12173	24285-24290	NN	denotes	Table
T12174	24291-24292	CD	denotes	3
T12175	24292-24293	-RRB-	denotes	)
T12176	24293-24294	.	denotes	.
T12177	24294-24422	sentence	denotes	Notably, both Pygo1 and Pygo2 were identified as downregulated (- 5.3-fold and - 3.9-fold, respectively) in the mutant kidneys.
T12178	24295-24302	RB	denotes	Notably
T12180	24302-24304	,	denotes	,
T12181	24304-24308	CC	denotes	both
T12182	24309-24314	NN	denotes	Pygo1
T12183	24315-24318	CC	denotes	and
T12184	24319-24324	NN	denotes	Pygo2
T12185	24325-24329	VBD	denotes	were
T12179	24330-24340	VBN	denotes	identified
T12186	24341-24343	IN	denotes	as
T12187	24344-24357	VBN	denotes	downregulated
T12188	24358-24359	-LRB-	denotes	(
T12190	24359-24360	SYM	denotes	-
T12191	24361-24369	RB	denotes	5.3-fold
T12192	24370-24373	CC	denotes	and
T12193	24374-24375	SYM	denotes	-
T12194	24376-24384	RB	denotes	3.9-fold
T12195	24384-24386	,	denotes	,
T12189	24386-24398	RB	denotes	respectively
T12196	24398-24399	-RRB-	denotes	)
T12197	24400-24402	IN	denotes	in
T12198	24403-24406	DT	denotes	the
T12200	24407-24413	NN	denotes	mutant
T12199	24414-24421	NNS	denotes	kidneys
T12201	24421-24422	.	denotes	.
T12202	24422-24556	sentence	denotes	Other genes with significant decreases in mutant kidneys included Cxcl13 (- 2.3-fold), Slc5a2 (- 2.8-fold), and Slco1a4 (- 2.1-fold).
T12203	24423-24428	JJ	denotes	Other
T12204	24429-24434	NNS	denotes	genes
T12206	24435-24439	IN	denotes	with
T12207	24440-24451	JJ	denotes	significant
T12208	24452-24461	NNS	denotes	decreases
T12209	24462-24464	IN	denotes	in
T12210	24465-24471	NN	denotes	mutant
T12211	24472-24479	NNS	denotes	kidneys
T12205	24480-24488	VBD	denotes	included
T12212	24489-24495	NN	denotes	Cxcl13
T12213	24496-24497	-LRB-	denotes	(
T12215	24497-24498	SYM	denotes	-
T12214	24499-24507	RB	denotes	2.3-fold
T12216	24507-24508	-RRB-	denotes	)
T12217	24508-24510	,	denotes	,
T12218	24510-24516	NN	denotes	Slc5a2
T12219	24517-24518	-LRB-	denotes	(
T12221	24518-24519	SYM	denotes	-
T12220	24520-24528	RB	denotes	2.8-fold
T12222	24528-24529	-RRB-	denotes	)
T12223	24529-24531	,	denotes	,
T12224	24531-24534	CC	denotes	and
T12225	24535-24542	NN	denotes	Slco1a4
T12226	24543-24544	-LRB-	denotes	(
T12228	24544-24545	SYM	denotes	-
T12227	24546-24554	RB	denotes	2.1-fold
T12229	24554-24555	-RRB-	denotes	)
T12230	24555-24556	.	denotes	.
T12231	24556-24747	sentence	denotes	Slc5a2 is expressed in the proximal tubules of the adult nephron and has been implicated in autosomal recessive renal glucosuria, characterized by loss of glucose uptake by the nephron [23].
T12232	24557-24563	NN	denotes	Slc5a2
T12234	24564-24566	VBZ	denotes	is
T12233	24567-24576	VBN	denotes	expressed
T12235	24577-24579	IN	denotes	in
T12236	24580-24583	DT	denotes	the
T12238	24584-24592	JJ	denotes	proximal
T12237	24593-24600	NNS	denotes	tubules
T12239	24601-24603	IN	denotes	of
T12240	24604-24607	DT	denotes	the
T12242	24608-24613	JJ	denotes	adult
T12241	24614-24621	NN	denotes	nephron
T12243	24622-24625	CC	denotes	and
T12244	24626-24629	VBZ	denotes	has
T12246	24630-24634	VBN	denotes	been
T12245	24635-24645	VBN	denotes	implicated
T12247	24646-24648	IN	denotes	in
T12248	24649-24658	JJ	denotes	autosomal
T12249	24659-24668	JJ	denotes	recessive
T12251	24669-24674	JJ	denotes	renal
T12250	24675-24685	NN	denotes	glucosuria
T12252	24685-24687	,	denotes	,
T12253	24687-24700	VBN	denotes	characterized
T12254	24701-24703	IN	denotes	by
T12255	24704-24708	NN	denotes	loss
T12256	24709-24711	IN	denotes	of
T12257	24712-24719	NN	denotes	glucose
T12258	24720-24726	NN	denotes	uptake
T12259	24727-24729	IN	denotes	by
T12260	24730-24733	DT	denotes	the
T12261	24734-24741	NN	denotes	nephron
T12262	24742-24743	-LRB-	denotes	[
T12263	24743-24745	CD	denotes	23
T12264	24745-24746	-RRB-	denotes	]
T12265	24746-24747	.	denotes	.
T12266	24747-24855	sentence	denotes	The organic anion transporter, Slco1a4, is also strongly expressed in the tubules of the adult kidney [24].
T12267	24748-24751	DT	denotes	The
T12269	24752-24759	JJ	denotes	organic
T12270	24760-24765	NN	denotes	anion
T12268	24766-24777	NN	denotes	transporter
T12272	24777-24779	,	denotes	,
T12273	24779-24786	NN	denotes	Slco1a4
T12274	24786-24788	,	denotes	,
T12275	24788-24790	VBZ	denotes	is
T12276	24791-24795	RB	denotes	also
T12277	24796-24804	RB	denotes	strongly
T12271	24805-24814	VBN	denotes	expressed
T12278	24815-24817	IN	denotes	in
T12279	24818-24821	DT	denotes	the
T12280	24822-24829	NNS	denotes	tubules
T12281	24830-24832	IN	denotes	of
T12282	24833-24836	DT	denotes	the
T12284	24837-24842	JJ	denotes	adult
T12283	24843-24849	NN	denotes	kidney
T12285	24850-24851	-LRB-	denotes	[
T12286	24851-24853	CD	denotes	24
T12287	24853-24854	-RRB-	denotes	]
T12288	24854-24855	.	denotes	.
T12289	24855-24969	sentence	denotes	These results suggest that the Pygo1/Pygo2 genes might play a role in nephron maturation and subsequent function.
T12290	24856-24861	DT	denotes	These
T12291	24862-24869	NNS	denotes	results
T12292	24870-24877	VBP	denotes	suggest
T12293	24878-24882	IN	denotes	that
T12295	24883-24886	DT	denotes	the
T12297	24887-24892	NN	denotes	Pygo1
T12299	24892-24893	HYPH	denotes	/
T12298	24893-24898	NN	denotes	Pygo2
T12296	24899-24904	NNS	denotes	genes
T12300	24905-24910	MD	denotes	might
T12294	24911-24915	VB	denotes	play
T12301	24916-24917	DT	denotes	a
T12302	24918-24922	NN	denotes	role
T12303	24923-24925	IN	denotes	in
T12304	24926-24933	NN	denotes	nephron
T12305	24934-24944	NN	denotes	maturation
T12306	24945-24948	CC	denotes	and
T12307	24949-24959	JJ	denotes	subsequent
T12308	24960-24968	NN	denotes	function
T12309	24968-24969	.	denotes	.
T12310	24969-24970	sentence	denotes
T27572	24979-24984	NNS	denotes	Genes
T27573	24985-24999	RB	denotes	differentially
T27574	25000-25009	VBN	denotes	expressed
T27575	25010-25011	-LRB-	denotes	(
T27577	25011-25012	NN	denotes	p
T27579	25013-25014	SYM	denotes	<
T27578	25015-25019	CD	denotes	0.05
T27580	25019-25021	,	denotes	,
T27581	25021-25029	JJ	denotes	two-fold
T27576	25030-25036	NN	denotes	change
T27582	25037-25039	CC	denotes	or
T27583	25040-25047	JJR	denotes	greater
T27584	25047-25048	-RRB-	denotes	)
T27585	25049-25051	IN	denotes	in
T27586	25052-25055	DT	denotes	the
T27588	25056-25061	NN	denotes	Pygo1
T27590	25061-25062	HYPH	denotes	/
T27589	25062-25067	NN	denotes	Pygo2
T27591	25068-25072	JJ	denotes	null
T27592	25073-25078	NN	denotes	E18.5
T27587	25079-25085	NN	denotes	kidney
T27593	25086-25096	VBN	denotes	normalized
T27594	25097-25099	IN	denotes	to
T27595	25100-25104	JJ	denotes	wild
T27597	25104-25105	HYPH	denotes	-
T27596	25105-25109	NN	denotes	type
T27598	25110-25117	NNS	denotes	samples
T27599	25117-25118	.	denotes	.
T12312	26990-27000	JJ	denotes	Noteworthy
T12311	26990-27134	sentence	denotes	Noteworthy genes upregulated in the Pygo1/Pygo2 null kidney included Klk5 (2.8-fold), Klk6 (2.7-fold), Ren2 (2.2-fold), and Timeless (2.4-fold).
T12313	27001-27006	NNS	denotes	genes
T12315	27007-27018	VBN	denotes	upregulated
T12316	27019-27021	IN	denotes	in
T12317	27022-27025	DT	denotes	the
T12319	27026-27031	NN	denotes	Pygo1
T12321	27031-27032	HYPH	denotes	/
T12320	27032-27037	NN	denotes	Pygo2
T12322	27038-27042	JJ	denotes	null
T12318	27043-27049	NN	denotes	kidney
T12314	27050-27058	VBN	denotes	included
T12323	27059-27063	NN	denotes	Klk5
T12324	27064-27065	-LRB-	denotes	(
T12325	27065-27073	RB	denotes	2.8-fold
T12326	27073-27074	-RRB-	denotes	)
T12327	27074-27076	,	denotes	,
T12328	27076-27080	NN	denotes	Klk6
T12329	27081-27082	-LRB-	denotes	(
T12330	27082-27090	RB	denotes	2.7-fold
T12331	27090-27091	-RRB-	denotes	)
T12332	27091-27093	,	denotes	,
T12333	27093-27097	NN	denotes	Ren2
T12334	27098-27099	-LRB-	denotes	(
T12335	27099-27107	RB	denotes	2.2-fold
T12336	27107-27108	-RRB-	denotes	)
T12337	27108-27110	,	denotes	,
T12338	27110-27113	CC	denotes	and
T12339	27114-27122	NNP	denotes	Timeless
T12340	27123-27124	-LRB-	denotes	(
T12341	27124-27132	RB	denotes	2.4-fold
T12342	27132-27133	-RRB-	denotes	)
T12343	27133-27134	.	denotes	.
T12344	27134-27219	sentence	denotes	Klk5 and Klk6 are members of the kallikrein family of trypsin-like serine proteases.
T12345	27135-27139	NN	denotes	Klk5
T12347	27140-27143	CC	denotes	and
T12348	27144-27148	NN	denotes	Klk6
T12346	27149-27152	VBP	denotes	are
T12349	27153-27160	NNS	denotes	members
T12350	27161-27163	IN	denotes	of
T12351	27164-27167	DT	denotes	the
T12353	27168-27178	NN	denotes	kallikrein
T12352	27179-27185	NN	denotes	family
T12354	27186-27188	IN	denotes	of
T12355	27189-27196	NN	denotes	trypsin
T12357	27196-27197	HYPH	denotes	-
T12356	27197-27201	JJ	denotes	like
T12359	27202-27208	NN	denotes	serine
T12358	27209-27218	NNS	denotes	proteases
T12360	27218-27219	.	denotes	.
T12361	27219-27323	sentence	denotes	Kallikreins have diverse functions in cancer, tissue remodeling, and regulation of blood pressure [25].
T12362	27220-27231	NNS	denotes	Kallikreins
T12363	27232-27236	VBP	denotes	have
T12364	27237-27244	JJ	denotes	diverse
T12365	27245-27254	NNS	denotes	functions
T12366	27255-27257	IN	denotes	in
T12367	27258-27264	NN	denotes	cancer
T12368	27264-27266	,	denotes	,
T12369	27266-27272	NN	denotes	tissue
T12370	27273-27283	NN	denotes	remodeling
T12371	27283-27285	,	denotes	,
T12372	27285-27288	CC	denotes	and
T12373	27289-27299	NN	denotes	regulation
T12374	27300-27302	IN	denotes	of
T12375	27303-27308	NN	denotes	blood
T12376	27309-27317	NN	denotes	pressure
T12377	27318-27319	-LRB-	denotes	[
T12378	27319-27321	CD	denotes	25
T12379	27321-27322	-RRB-	denotes	]
T12380	27322-27323	.	denotes	.
T12381	27323-27441	sentence	denotes	Ren2, a homolog of the endopeptidase Renin 1, activates the renin-angiotensin system, increasing blood pressure [26].
T12382	27324-27328	NN	denotes	Ren2
T12384	27328-27330	,	denotes	,
T12385	27330-27331	DT	denotes	a
T12386	27332-27339	NN	denotes	homolog
T12387	27340-27342	IN	denotes	of
T12388	27343-27346	DT	denotes	the
T12389	27347-27360	NN	denotes	endopeptidase
T12390	27361-27366	NN	denotes	Renin
T12391	27367-27368	CD	denotes	1
T12392	27368-27370	,	denotes	,
T12383	27370-27379	VBZ	denotes	activates
T12393	27380-27383	DT	denotes	the
T12395	27384-27389	NN	denotes	renin
T12397	27389-27390	HYPH	denotes	-
T12396	27390-27401	NN	denotes	angiotensin
T12394	27402-27408	NN	denotes	system
T12398	27408-27410	,	denotes	,
T12399	27410-27420	VBG	denotes	increasing
T12400	27421-27426	NN	denotes	blood
T12401	27427-27435	NN	denotes	pressure
T12402	27436-27437	-LRB-	denotes	[
T12403	27437-27439	CD	denotes	26
T12404	27439-27440	-RRB-	denotes	]
T12405	27440-27441	.	denotes	.
T12406	27441-27586	sentence	denotes	Disruption of the renin-angiotensin system during development results in congenital abnormalities of the ureter and collecting-duct system [27].
T12407	27442-27452	NN	denotes	Disruption
T12409	27453-27455	IN	denotes	of
T12410	27456-27459	DT	denotes	the
T12412	27460-27465	NN	denotes	renin
T12414	27465-27466	HYPH	denotes	-
T12413	27466-27477	NN	denotes	angiotensin
T12411	27478-27484	NN	denotes	system
T12415	27485-27491	IN	denotes	during
T12416	27492-27503	NN	denotes	development
T12408	27504-27511	VBZ	denotes	results
T12417	27512-27514	IN	denotes	in
T12418	27515-27525	JJ	denotes	congenital
T12419	27526-27539	NNS	denotes	abnormalities
T12420	27540-27542	IN	denotes	of
T12421	27543-27546	DT	denotes	the
T12422	27547-27553	NN	denotes	ureter
T12423	27554-27557	CC	denotes	and
T12424	27558-27568	NN	denotes	collecting
T12426	27568-27569	HYPH	denotes	-
T12425	27569-27573	NN	denotes	duct
T12427	27574-27580	NN	denotes	system
T12428	27581-27582	-LRB-	denotes	[
T12429	27582-27584	CD	denotes	27
T12430	27584-27585	-RRB-	denotes	]
T12431	27585-27586	.	denotes	.
T12432	27586-27785	sentence	denotes	Timeless, a transcription factor involved in the regulation of circadian rhythms, and expressed in the branching ureteric tips, has also been shown to regulate ureteric branching morphogenesis [28].
T12433	27587-27595	NN	denotes	Timeless
T12435	27595-27597	,	denotes	,
T12436	27597-27598	DT	denotes	a
T12438	27599-27612	NN	denotes	transcription
T12437	27613-27619	NN	denotes	factor
T12439	27620-27628	VBN	denotes	involved
T12440	27629-27631	IN	denotes	in
T12441	27632-27635	DT	denotes	the
T12442	27636-27646	NN	denotes	regulation
T12443	27647-27649	IN	denotes	of
T12444	27650-27659	JJ	denotes	circadian
T12445	27660-27667	NNS	denotes	rhythms
T12446	27667-27669	,	denotes	,
T12447	27669-27672	CC	denotes	and
T12448	27673-27682	VBN	denotes	expressed
T12449	27683-27685	IN	denotes	in
T12450	27686-27689	DT	denotes	the
T12452	27690-27699	VBG	denotes	branching
T12453	27700-27708	JJ	denotes	ureteric
T12451	27709-27713	NNS	denotes	tips
T12454	27713-27715	,	denotes	,
T12455	27715-27718	VBZ	denotes	has
T12456	27719-27723	RB	denotes	also
T12457	27724-27728	VBN	denotes	been
T12434	27729-27734	VBN	denotes	shown
T12458	27735-27737	TO	denotes	to
T12459	27738-27746	VB	denotes	regulate
T12460	27747-27755	JJ	denotes	ureteric
T12462	27756-27765	NN	denotes	branching
T12461	27766-27779	NN	denotes	morphogenesis
T12463	27780-27781	-LRB-	denotes	[
T12464	27781-27783	CD	denotes	28
T12465	27783-27784	-RRB-	denotes	]
T12466	27784-27785	.	denotes	.
T12467	27785-27912	sentence	denotes	We were interested in the possible altered expression of previously known Wnt targets in the Pygo1-/-/Pygo2-/- mutant kidneys.
T12468	27786-27788	PRP	denotes	We
T12469	27789-27793	VBD	denotes	were
T12470	27794-27804	JJ	denotes	interested
T12471	27805-27807	IN	denotes	in
T12472	27808-27811	DT	denotes	the
T12474	27812-27820	JJ	denotes	possible
T12475	27821-27828	VBN	denotes	altered
T12473	27829-27839	NN	denotes	expression
T12476	27840-27842	IN	denotes	of
T12477	27843-27853	RB	denotes	previously
T12478	27854-27859	VBN	denotes	known
T12480	27860-27863	NN	denotes	Wnt
T12479	27864-27871	NNS	denotes	targets
T12481	27872-27874	IN	denotes	in
T12482	27875-27878	DT	denotes	the
T12484	27879-27884	NN	denotes	Pygo1
T12486	27884-27885	SYM	denotes	-
T12487	27885-27886	HYPH	denotes	/
T12488	27886-27887	SYM	denotes	-
T12489	27887-27888	HYPH	denotes	/
T12490	27888-27893	NN	denotes	Pygo2
T12491	27893-27894	SYM	denotes	-
T12492	27894-27895	HYPH	denotes	/
T12493	27895-27896	SYM	denotes	-
T12485	27897-27903	NN	denotes	mutant
T12483	27904-27911	NNS	denotes	kidneys
T12494	27911-27912	.	denotes	.
T12495	27912-27960	sentence	denotes	A list of known Wnt targets was compiled from .
T12496	27913-27914	DT	denotes	A
T12497	27915-27919	NN	denotes	list
T12499	27920-27922	IN	denotes	of
T12500	27923-27928	JJ	denotes	known
T12502	27929-27932	NN	denotes	Wnt
T12501	27933-27940	NNS	denotes	targets
T12503	27941-27944	VBD	denotes	was
T12498	27945-27953	VBN	denotes	compiled
T12504	27954-27958	IN	denotes	from
T12505	27959-27960	.	denotes	.
T12506	27960-28063	sentence	denotes	Both Pygo1 and Pygo2 probe sets were included as references to illustrate significant down regulation.
T12507	27961-27965	CC	denotes	Both
T12508	27966-27971	NN	denotes	Pygo1
T12510	27972-27975	CC	denotes	and
T12511	27976-27981	NN	denotes	Pygo2
T12512	27982-27987	NN	denotes	probe
T12509	27988-27992	NNS	denotes	sets
T12514	27993-27997	VBD	denotes	were
T12513	27998-28006	VBN	denotes	included
T12515	28007-28009	IN	denotes	as
T12516	28010-28020	NNS	denotes	references
T12517	28021-28023	TO	denotes	to
T12518	28024-28034	VB	denotes	illustrate
T12519	28035-28046	JJ	denotes	significant
T12521	28047-28051	JJ	denotes	down
T12520	28052-28062	NN	denotes	regulation
T12522	28062-28063	.	denotes	.
T12523	28063-28193	sentence	denotes	Remarkably, the known Wnt target genes showed very few differences in expression between wild-type and mutant kidneys (Figure 8).
T12524	28064-28074	RB	denotes	Remarkably
T12526	28074-28076	,	denotes	,
T12527	28076-28079	DT	denotes	the
T12529	28080-28085	VBN	denotes	known
T12530	28086-28089	NN	denotes	Wnt
T12531	28090-28096	NN	denotes	target
T12528	28097-28102	NNS	denotes	genes
T12525	28103-28109	VBD	denotes	showed
T12532	28110-28114	RB	denotes	very
T12533	28115-28118	JJ	denotes	few
T12534	28119-28130	NNS	denotes	differences
T12535	28131-28133	IN	denotes	in
T12536	28134-28144	NN	denotes	expression
T12537	28145-28152	IN	denotes	between
T12538	28153-28157	JJ	denotes	wild
T12540	28157-28158	HYPH	denotes	-
T12539	28158-28162	NN	denotes	type
T12542	28163-28166	CC	denotes	and
T12543	28167-28173	NN	denotes	mutant
T12541	28174-28181	NNS	denotes	kidneys
T12544	28182-28183	-LRB-	denotes	(
T12545	28183-28189	NN	denotes	Figure
T12546	28190-28191	CD	denotes	8
T12547	28191-28192	-RRB-	denotes	)
T12548	28192-28193	.	denotes	.
T12549	28193-28362	sentence	denotes	Only the expression of Ccnd1 encoding cyclin D1 [29], and Wisp1 [30] were significantly changed, with expression level changes of <1.5-fold in Pygo1/Pygo2 null kidneys.
T12550	28194-28198	RB	denotes	Only
T12552	28199-28202	DT	denotes	the
T12551	28203-28213	NN	denotes	expression
T12554	28214-28216	IN	denotes	of
T12555	28217-28222	NN	denotes	Ccnd1
T12556	28223-28231	VBG	denotes	encoding
T12558	28232-28238	NN	denotes	cyclin
T12557	28239-28241	NN	denotes	D1
T12559	28242-28243	-LRB-	denotes	[
T12560	28243-28245	CD	denotes	29
T12561	28245-28246	-RRB-	denotes	]
T12562	28246-28248	,	denotes	,
T12563	28248-28251	CC	denotes	and
T12564	28252-28257	NN	denotes	Wisp1
T12565	28258-28259	-LRB-	denotes	[
T12566	28259-28261	CD	denotes	30
T12567	28261-28262	-RRB-	denotes	]
T12568	28263-28267	VBD	denotes	were
T12569	28268-28281	RB	denotes	significantly
T12553	28282-28289	VBN	denotes	changed
T12570	28289-28291	,	denotes	,
T12571	28291-28295	IN	denotes	with
T12572	28296-28306	NN	denotes	expression
T12573	28307-28312	NN	denotes	level
T12574	28313-28320	NNS	denotes	changes
T12575	28321-28323	IN	denotes	of
T12576	28324-28325	SYM	denotes	<
T12577	28325-28333	RB	denotes	1.5-fold
T12578	28334-28336	IN	denotes	in
T12579	28337-28342	NN	denotes	Pygo1
T12581	28342-28343	HYPH	denotes	/
T12580	28343-28348	NN	denotes	Pygo2
T12582	28349-28353	JJ	denotes	null
T12583	28354-28361	NNS	denotes	kidneys
T12584	28361-28362	.	denotes	.
T12585	28362-28512	sentence	denotes	These results indicate an absence of dramatic changes in expression of previously known Wnt signaling target genes in the Pygo1/Pygo2 mutant kidneys.
T12586	28363-28368	DT	denotes	These
T12587	28369-28376	NNS	denotes	results
T12588	28377-28385	VBP	denotes	indicate
T12589	28386-28388	DT	denotes	an
T12590	28389-28396	NN	denotes	absence
T12591	28397-28399	IN	denotes	of
T12592	28400-28408	JJ	denotes	dramatic
T12593	28409-28416	NNS	denotes	changes
T12594	28417-28419	IN	denotes	in
T12595	28420-28430	NN	denotes	expression
T12596	28431-28433	IN	denotes	of
T12597	28434-28444	RB	denotes	previously
T12598	28445-28450	VBN	denotes	known
T12600	28451-28454	NN	denotes	Wnt
T12601	28455-28464	NN	denotes	signaling
T12602	28465-28471	NN	denotes	target
T12599	28472-28477	NNS	denotes	genes
T12603	28478-28480	IN	denotes	in
T12604	28481-28484	DT	denotes	the
T12606	28485-28490	NN	denotes	Pygo1
T12608	28490-28491	HYPH	denotes	/
T12607	28491-28496	NN	denotes	Pygo2
T12609	28497-28503	NN	denotes	mutant
T12605	28504-28511	NNS	denotes	kidneys
T12610	28511-28512	.	denotes	.
T12611	28512-29187	sentence	denotes	Figure 8 Gene expression changes of common Wnt signaling targets in the E18.5 Pygo1/Pygo2 null kidney. Microarray analysis was performed in triplicate on wild-type and Pygo1/2 compound null E18.5 kidneys. Possible Wnt targets were selected from those compiled at [39]. An initial gene list of 82 Wnt targets was created and then reduced to a total of 40 genes using an expression level restriction requiring the raw expression intensity to be >100 in at least 3 samples. Pygo1 and Pygo2 probes were included to demonstrate significant changes in expression levels. We used quantitative real-time PCR, with independent biological samples, to validate the microarray results.
T27272	28523-28527	NN	denotes	Gene
T27273	28528-28538	NN	denotes	expression
T27274	28539-28546	NNS	denotes	changes
T27275	28547-28549	IN	denotes	of
T27276	28550-28556	JJ	denotes	common
T27278	28557-28560	NN	denotes	Wnt
T27279	28561-28570	NN	denotes	signaling
T27277	28571-28578	NNS	denotes	targets
T27280	28579-28581	IN	denotes	in
T27281	28582-28585	DT	denotes	the
T27283	28586-28591	NN	denotes	E18.5
T27284	28592-28597	NN	denotes	Pygo1
T27286	28597-28598	HYPH	denotes	/
T27285	28598-28603	NN	denotes	Pygo2
T27287	28604-28608	JJ	denotes	null
T27282	28609-28615	NN	denotes	kidney
T27288	28615-28616	.	denotes	.
T27289	28616-28718	sentence	denotes	Microarray analysis was performed in triplicate on wild-type and Pygo1/2 compound null E18.5 kidneys.
T27290	28617-28627	NN	denotes	Microarray
T27291	28628-28636	NN	denotes	analysis
T27293	28637-28640	VBD	denotes	was
T27292	28641-28650	VBN	denotes	performed
T27294	28651-28653	IN	denotes	in
T27295	28654-28664	NN	denotes	triplicate
T27296	28665-28667	IN	denotes	on
T27297	28668-28672	JJ	denotes	wild
T27299	28672-28673	HYPH	denotes	-
T27298	28673-28677	NN	denotes	type
T27301	28678-28681	CC	denotes	and
T27302	28682-28687	NN	denotes	Pygo1
T27304	28687-28688	HYPH	denotes	/
T27305	28688-28689	CD	denotes	2
T27306	28690-28698	NN	denotes	compound
T27303	28699-28703	JJ	denotes	null
T27307	28704-28709	NN	denotes	E18.5
T27300	28710-28717	NNS	denotes	kidneys
T27308	28717-28718	.	denotes	.
T27309	28718-28782	sentence	denotes	Possible Wnt targets were selected from those compiled at [39].
T27310	28719-28727	JJ	denotes	Possible
T27312	28728-28731	NN	denotes	Wnt
T27311	28732-28739	NNS	denotes	targets
T27314	28740-28744	VBD	denotes	were
T27313	28745-28753	VBN	denotes	selected
T27315	28754-28758	IN	denotes	from
T27316	28759-28764	DT	denotes	those
T27317	28765-28773	VBN	denotes	compiled
T27318	28774-28776	IN	denotes	at
T27319	28777-28778	-LRB-	denotes	[
T27320	28778-28780	CD	denotes	39
T27321	28780-28781	-RRB-	denotes	]
T27322	28781-28782	.	denotes	.
T27323	28782-28984	sentence	denotes	An initial gene list of 82 Wnt targets was created and then reduced to a total of 40 genes using an expression level restriction requiring the raw expression intensity to be >100 in at least 3 samples.
T27324	28783-28785	DT	denotes	An
T27326	28786-28793	JJ	denotes	initial
T27327	28794-28798	NN	denotes	gene
T27325	28799-28803	NN	denotes	list
T27329	28804-28806	IN	denotes	of
T27330	28807-28809	CD	denotes	82
T27332	28810-28813	NN	denotes	Wnt
T27331	28814-28821	NNS	denotes	targets
T27333	28822-28825	VBD	denotes	was
T27328	28826-28833	VBN	denotes	created
T27334	28834-28837	CC	denotes	and
T27335	28838-28842	RB	denotes	then
T27336	28843-28850	VBN	denotes	reduced
T27337	28851-28853	IN	denotes	to
T27338	28854-28855	DT	denotes	a
T27339	28856-28861	NN	denotes	total
T27340	28862-28864	IN	denotes	of
T27341	28865-28867	CD	denotes	40
T27342	28868-28873	NNS	denotes	genes
T27343	28874-28879	VBG	denotes	using
T27344	28880-28882	DT	denotes	an
T27346	28883-28893	NN	denotes	expression
T27347	28894-28899	NN	denotes	level
T27345	28900-28911	NN	denotes	restriction
T27348	28912-28921	VBG	denotes	requiring
T27349	28922-28925	DT	denotes	the
T27351	28926-28929	JJ	denotes	raw
T27352	28930-28940	NN	denotes	expression
T27350	28941-28950	NN	denotes	intensity
T27353	28951-28953	TO	denotes	to
T27354	28954-28956	VB	denotes	be
T27355	28957-28958	SYM	denotes	>
T27356	28958-28961	CD	denotes	100
T27357	28962-28964	IN	denotes	in
T27358	28965-28967	RB	denotes	at
T27360	28968-28973	RBS	denotes	least
T27359	28974-28975	CD	denotes	3
T27361	28976-28983	NNS	denotes	samples
T27362	28983-28984	.	denotes	.
T27363	28984-29078	sentence	denotes	Pygo1 and Pygo2 probes were included to demonstrate significant changes in expression levels.
T27364	28985-28990	NN	denotes	Pygo1
T27366	28991-28994	CC	denotes	and
T27367	28995-29000	NN	denotes	Pygo2
T27365	29001-29007	NNS	denotes	probes
T27369	29008-29012	VBD	denotes	were
T27368	29013-29021	VBN	denotes	included
T27370	29022-29024	TO	denotes	to
T27371	29025-29036	VB	denotes	demonstrate
T27372	29037-29048	JJ	denotes	significant
T27373	29049-29056	NNS	denotes	changes
T27374	29057-29059	IN	denotes	in
T27375	29060-29070	NN	denotes	expression
T27376	29071-29077	NNS	denotes	levels
T27377	29077-29078	.	denotes	.
T12612	29079-29081	PRP	denotes	We
T12613	29082-29086	VBD	denotes	used
T12614	29087-29099	JJ	denotes	quantitative
T12616	29100-29104	JJ	denotes	real
T12618	29104-29105	HYPH	denotes	-
T12617	29105-29109	NN	denotes	time
T12615	29110-29113	NN	denotes	PCR
T12619	29113-29115	,	denotes	,
T12620	29115-29119	IN	denotes	with
T12621	29120-29131	JJ	denotes	independent
T12623	29132-29142	JJ	denotes	biological
T12622	29143-29150	NNS	denotes	samples
T12624	29150-29152	,	denotes	,
T12625	29152-29154	TO	denotes	to
T12626	29155-29163	VB	denotes	validate
T12627	29164-29167	DT	denotes	the
T12629	29168-29178	NN	denotes	microarray
T12628	29179-29186	NNS	denotes	results
T12630	29186-29187	.	denotes	.
T12631	29187-29335	sentence	denotes	Nine genes were tested, and for seven we did observe a significant fold change in the same direction predicted by the microarray results (Table 4).
T12632	29188-29192	CD	denotes	Nine
T12633	29193-29198	NNS	denotes	genes
T12635	29199-29203	VBD	denotes	were
T12634	29204-29210	VBN	denotes	tested
T12636	29210-29212	,	denotes	,
T12637	29212-29215	CC	denotes	and
T12638	29216-29219	IN	denotes	for
T12640	29220-29225	CD	denotes	seven
T12641	29226-29228	PRP	denotes	we
T12642	29229-29232	VBD	denotes	did
T12639	29233-29240	VB	denotes	observe
T12643	29241-29242	DT	denotes	a
T12645	29243-29254	JJ	denotes	significant
T12646	29255-29259	NN	denotes	fold
T12644	29260-29266	NN	denotes	change
T12647	29267-29269	IN	denotes	in
T12648	29270-29273	DT	denotes	the
T12650	29274-29278	JJ	denotes	same
T12649	29279-29288	NN	denotes	direction
T12651	29289-29298	VBN	denotes	predicted
T12652	29299-29301	IN	denotes	by
T12653	29302-29305	DT	denotes	the
T12655	29306-29316	NN	denotes	microarray
T12654	29317-29324	NNS	denotes	results
T12656	29325-29326	-LRB-	denotes	(
T12657	29326-29331	NN	denotes	Table
T12658	29332-29333	CD	denotes	4
T12659	29333-29334	-RRB-	denotes	)
T12660	29334-29335	.	denotes	.
T12661	29335-29488	sentence	denotes	It is not surprising that two of the nine genes could not be validated, considering the relatively low stringency used in screening the microarray data.
T12662	29336-29338	PRP	denotes	It
T12663	29339-29341	VBZ	denotes	is
T12664	29342-29345	RB	denotes	not
T12665	29346-29356	JJ	denotes	surprising
T12666	29357-29361	IN	denotes	that
T12668	29362-29365	CD	denotes	two
T12669	29366-29368	IN	denotes	of
T12670	29369-29372	DT	denotes	the
T12672	29373-29377	CD	denotes	nine
T12671	29378-29383	NNS	denotes	genes
T12673	29384-29389	MD	denotes	could
T12674	29390-29393	RB	denotes	not
T12675	29394-29396	VB	denotes	be
T12667	29397-29406	VBN	denotes	validated
T12676	29406-29408	,	denotes	,
T12677	29408-29419	VBG	denotes	considering
T12678	29420-29423	DT	denotes	the
T12680	29424-29434	RB	denotes	relatively
T12681	29435-29438	JJ	denotes	low
T12679	29439-29449	NN	denotes	stringency
T12682	29450-29454	VBN	denotes	used
T12683	29455-29457	IN	denotes	in
T12684	29458-29467	VBG	denotes	screening
T12685	29468-29471	DT	denotes	the
T12687	29472-29482	NN	denotes	microarray
T12686	29483-29487	NNS	denotes	data
T12688	29487-29488	.	denotes	.
T27655	29498-29508	NN	denotes	Validation
T27656	29509-29511	IN	denotes	of
T27657	29512-29516	NN	denotes	gene
T27658	29517-29527	NN	denotes	expression
T27659	29528-29535	NNS	denotes	changes
T27660	29536-29538	IN	denotes	in
T27661	29539-29542	DT	denotes	the
T27663	29543-29548	NN	denotes	E18.5
T27664	29549-29554	NN	denotes	Pygo1
T27666	29554-29555	HYPH	denotes	/
T27665	29555-29560	NN	denotes	Pygo2
T27667	29561-29565	JJ	denotes	null
T27662	29566-29573	NNS	denotes	kidneys
T27668	29574-29584	VBN	denotes	normalized
T27669	29585-29587	IN	denotes	to
T27670	29588-29593	NN	denotes	E18.5
T27672	29594-29598	JJ	denotes	wild
T27674	29598-29599	HYPH	denotes	-
T27673	29599-29603	NN	denotes	type
T27671	29604-29611	NNS	denotes	kidneys
T27675	29611-29612	.	denotes	.
T15289	29972-29974	IN	denotes	In
T15291	29975-29985	NNP	denotes	Drosophila
T15292	29985-29987	,	denotes	,
T15293	29987-29990	DT	denotes	the
T15295	29991-29998	NN	denotes	Pygopus
T15294	29999-30003	NN	denotes	gene
T15290	30004-30006	VBZ	denotes	is
T15296	30007-30008	DT	denotes	a
T15298	30009-30012	JJ	denotes	key
T15297	30013-30021	NN	denotes	mediator
T15299	30022-30024	IN	denotes	of
T15300	30025-30034	JJ	denotes	canonical
T15302	30035-30038	NN	denotes	Wnt
T15301	30039-30048	NN	denotes	signaling
T15303	30048-30049	.	denotes	.
T15304	30049-30190	sentence	denotes	In one study, 12 distinct measures of Wnt signaling in Pygo mutants were performed, including analysis of leg, wing, and eye imaginal discs.
T15305	30050-30052	IN	denotes	In
T15307	30053-30056	CD	denotes	one
T15308	30057-30062	NN	denotes	study
T15309	30062-30064	,	denotes	,
T15310	30064-30066	CD	denotes	12
T15312	30067-30075	JJ	denotes	distinct
T15311	30076-30084	NNS	denotes	measures
T15313	30085-30087	IN	denotes	of
T15314	30088-30091	NN	denotes	Wnt
T15315	30092-30101	NN	denotes	signaling
T15316	30102-30104	IN	denotes	in
T15317	30105-30109	NN	denotes	Pygo
T15318	30110-30117	NNS	denotes	mutants
T15319	30118-30122	VBD	denotes	were
T15306	30123-30132	VBN	denotes	performed
T15320	30132-30134	,	denotes	,
T15321	30134-30143	VBG	denotes	including
T15322	30144-30152	NN	denotes	analysis
T15323	30153-30155	IN	denotes	of
T15324	30156-30159	NN	denotes	leg
T15326	30159-30161	,	denotes	,
T15327	30161-30165	NN	denotes	wing
T15328	30165-30167	,	denotes	,
T15329	30167-30170	CC	denotes	and
T15330	30171-30174	NN	denotes	eye
T15325	30175-30183	JJ	denotes	imaginal
T15331	30184-30189	NNS	denotes	discs
T15332	30189-30190	.	denotes	.
T15333	30190-30291	sentence	denotes	In 2 cases there was a significant reduction of Wnt signaling and in 10 cases a complete block [10].
T15334	30191-30193	IN	denotes	In
T15336	30194-30195	CD	denotes	2
T15337	30196-30201	NNS	denotes	cases
T15338	30202-30207	EX	denotes	there
T15335	30208-30211	VBD	denotes	was
T15339	30212-30213	DT	denotes	a
T15341	30214-30225	JJ	denotes	significant
T15340	30226-30235	NN	denotes	reduction
T15342	30236-30238	IN	denotes	of
T15343	30239-30242	NN	denotes	Wnt
T15344	30243-30252	NN	denotes	signaling
T15345	30253-30256	CC	denotes	and
T15346	30257-30259	IN	denotes	in
T15348	30260-30262	CD	denotes	10
T15349	30263-30268	NNS	denotes	cases
T15350	30269-30270	DT	denotes	a
T15351	30271-30279	JJ	denotes	complete
T15347	30280-30285	NN	denotes	block
T15352	30286-30287	-LRB-	denotes	[
T15353	30287-30289	CD	denotes	10
T15354	30289-30290	-RRB-	denotes	]
T15355	30290-30291	.	denotes	.
T15356	30291-30547	sentence	denotes	A second study in Drosophila examined the effects of Pygo mutation on cuticle patterning, midgut constriction, central nervous system, and cardiac development, and concluded that "Pygo is an essential component in the Wg signal transduction pathway". [8].
T15357	30292-30293	DT	denotes	A
T15359	30294-30300	JJ	denotes	second
T15358	30301-30306	NN	denotes	study
T15361	30307-30309	IN	denotes	in
T15362	30310-30320	NNP	denotes	Drosophila
T15360	30321-30329	VBD	denotes	examined
T15363	30330-30333	DT	denotes	the
T15364	30334-30341	NNS	denotes	effects
T15365	30342-30344	IN	denotes	of
T15366	30345-30349	NN	denotes	Pygo
T15367	30350-30358	NN	denotes	mutation
T15368	30359-30361	IN	denotes	on
T15369	30362-30369	NN	denotes	cuticle
T15370	30370-30380	NN	denotes	patterning
T15371	30380-30382	,	denotes	,
T15372	30382-30388	NN	denotes	midgut
T15373	30389-30401	NN	denotes	constriction
T15374	30401-30403	,	denotes	,
T15375	30403-30410	JJ	denotes	central
T15377	30411-30418	JJ	denotes	nervous
T15376	30419-30425	NN	denotes	system
T15378	30425-30427	,	denotes	,
T15379	30427-30430	CC	denotes	and
T15380	30431-30438	JJ	denotes	cardiac
T15381	30439-30450	NN	denotes	development
T15382	30450-30452	,	denotes	,
T15383	30452-30455	CC	denotes	and
T15384	30456-30465	VBD	denotes	concluded
T15385	30466-30470	IN	denotes	that
T15387	30471-30472	``	denotes	"
T15388	30472-30476	NN	denotes	Pygo
T15386	30477-30479	VBZ	denotes	is
T15389	30480-30482	DT	denotes	an
T15391	30483-30492	JJ	denotes	essential
T15390	30493-30502	NN	denotes	component
T15392	30503-30505	IN	denotes	in
T15393	30506-30509	DT	denotes	the
T15395	30510-30512	NN	denotes	Wg
T15396	30513-30519	NN	denotes	signal
T15397	30520-30532	NN	denotes	transduction
T15394	30533-30540	NN	denotes	pathway
T15398	30540-30541	''	denotes	"
T15399	30541-30542	.	denotes	.
T15400	30543-30544	-LRB-	denotes	[
T15401	30544-30545	CD	denotes	8
T15402	30545-30546	-RRB-	denotes	]
T15403	30546-30547	.	denotes	.
T15404	30547-30696	sentence	denotes	Given these results in Drosophila, the relatively mild phenotypes of mice with targeted Pygo1, Pygo2, or double Pygo1/Pygo2 mutations were striking.
T15405	30548-30553	VBN	denotes	Given
T15407	30554-30559	DT	denotes	these
T15408	30560-30567	NNS	denotes	results
T15409	30568-30570	IN	denotes	in
T15410	30571-30581	NNP	denotes	Drosophila
T15411	30581-30583	,	denotes	,
T15412	30583-30586	DT	denotes	the
T15414	30587-30597	RB	denotes	relatively
T15415	30598-30602	JJ	denotes	mild
T15413	30603-30613	NNS	denotes	phenotypes
T15416	30614-30616	IN	denotes	of
T15417	30617-30621	NNS	denotes	mice
T15418	30622-30626	IN	denotes	with
T15419	30627-30635	VBN	denotes	targeted
T15420	30636-30641	NN	denotes	Pygo1
T15422	30641-30643	,	denotes	,
T15423	30643-30648	NN	denotes	Pygo2
T15424	30648-30650	,	denotes	,
T15425	30650-30652	CC	denotes	or
T15426	30653-30659	JJ	denotes	double
T15428	30660-30665	NN	denotes	Pygo1
T15429	30665-30666	HYPH	denotes	/
T15427	30666-30671	NN	denotes	Pygo2
T15421	30672-30681	NNS	denotes	mutations
T15406	30682-30686	VBD	denotes	were
T15430	30687-30695	JJ	denotes	striking
T15431	30695-30696	.	denotes	.
T15432	30696-30819	sentence	denotes	Pygo2 mutants developed to birth and showed limited abnormalities, while Pygo1 homozygous mutants were normal and fertile.
T15433	30697-30702	NN	denotes	Pygo2
T15434	30703-30710	NNS	denotes	mutants
T15435	30711-30720	VBD	denotes	developed
T15436	30721-30723	IN	denotes	to
T15437	30724-30729	NN	denotes	birth
T15438	30730-30733	CC	denotes	and
T15439	30734-30740	VBD	denotes	showed
T15440	30741-30748	JJ	denotes	limited
T15441	30749-30762	NNS	denotes	abnormalities
T15442	30762-30764	,	denotes	,
T15443	30764-30769	IN	denotes	while
T15445	30770-30775	NN	denotes	Pygo1
T15446	30776-30786	JJ	denotes	homozygous
T15447	30787-30794	NNS	denotes	mutants
T15444	30795-30799	VBD	denotes	were
T15448	30800-30806	JJ	denotes	normal
T15449	30807-30810	CC	denotes	and
T15450	30811-30818	JJ	denotes	fertile
T15451	30818-30819	.	denotes	.
T15452	30819-31016	sentence	denotes	Furthermore, there was no detected synergism in the phenotype of the double Pygo1/Pygo2 mutant, although in several tissues the BAT-gal Wnt reporter did show more a severe reduction in expression.
T15453	30820-30831	RB	denotes	Furthermore
T15455	30831-30833	,	denotes	,
T15456	30833-30838	EX	denotes	there
T15454	30839-30842	VBD	denotes	was
T15457	30843-30845	DT	denotes	no
T15459	30846-30854	VBN	denotes	detected
T15458	30855-30864	NN	denotes	synergism
T15460	30865-30867	IN	denotes	in
T15461	30868-30871	DT	denotes	the
T15462	30872-30881	NN	denotes	phenotype
T15463	30882-30884	IN	denotes	of
T15464	30885-30888	DT	denotes	the
T15466	30889-30895	JJ	denotes	double
T15467	30896-30901	NN	denotes	Pygo1
T15469	30901-30902	HYPH	denotes	/
T15468	30902-30907	NN	denotes	Pygo2
T15465	30908-30914	NN	denotes	mutant
T15470	30914-30916	,	denotes	,
T15471	30916-30924	IN	denotes	although
T15473	30925-30927	IN	denotes	in
T15474	30928-30935	JJ	denotes	several
T15475	30936-30943	NNS	denotes	tissues
T15476	30944-30947	DT	denotes	the
T15478	30948-30951	NN	denotes	BAT
T15480	30951-30952	HYPH	denotes	-
T15479	30952-30955	NN	denotes	gal
T15481	30956-30959	NN	denotes	Wnt
T15477	30960-30968	NN	denotes	reporter
T15482	30969-30972	VBD	denotes	did
T15472	30973-30977	VB	denotes	show
T15483	30978-30982	RBR	denotes	more
T15484	30983-30984	DT	denotes	a
T15486	30985-30991	JJ	denotes	severe
T15485	30992-31001	NN	denotes	reduction
T15487	31002-31004	IN	denotes	in
T15488	31005-31015	NN	denotes	expression
T15489	31015-31016	.	denotes	.
T15490	31016-31159	sentence	denotes	One possible explanation of these unexpected results is a failure of the gene targeting to eliminate functioning of the Pygo1 and Pygo2 genes.
T15491	31017-31020	CD	denotes	One
T15493	31021-31029	JJ	denotes	possible
T15492	31030-31041	NN	denotes	explanation
T15495	31042-31044	IN	denotes	of
T15496	31045-31050	DT	denotes	these
T15498	31051-31061	JJ	denotes	unexpected
T15497	31062-31069	NNS	denotes	results
T15494	31070-31072	VBZ	denotes	is
T15499	31073-31074	DT	denotes	a
T15500	31075-31082	NN	denotes	failure
T15501	31083-31085	IN	denotes	of
T15502	31086-31089	DT	denotes	the
T15503	31090-31094	NN	denotes	gene
T15504	31095-31104	VBG	denotes	targeting
T15505	31105-31107	TO	denotes	to
T15506	31108-31117	VB	denotes	eliminate
T15507	31118-31129	NN	denotes	functioning
T15508	31130-31132	IN	denotes	of
T15509	31133-31136	DT	denotes	the
T15511	31137-31142	NN	denotes	Pygo1
T15512	31143-31146	CC	denotes	and
T15513	31147-31152	NN	denotes	Pygo2
T15510	31153-31158	NNS	denotes	genes
T15514	31158-31159	.	denotes	.
T15515	31159-31262	sentence	denotes	The Pygopus deletion alleles described in this report, however, are almost certainly functional nulls.
T15516	31160-31163	DT	denotes	The
T15518	31164-31171	NN	denotes	Pygopus
T15519	31172-31180	NN	denotes	deletion
T15517	31181-31188	NNS	denotes	alleles
T15521	31189-31198	VBN	denotes	described
T15522	31199-31201	IN	denotes	in
T15523	31202-31206	DT	denotes	this
T15524	31207-31213	NN	denotes	report
T15525	31213-31215	,	denotes	,
T15526	31215-31222	RB	denotes	however
T15527	31222-31224	,	denotes	,
T15520	31224-31227	VBP	denotes	are
T15528	31228-31234	RB	denotes	almost
T15529	31235-31244	RB	denotes	certainly
T15530	31245-31255	JJ	denotes	functional
T15531	31256-31261	NNS	denotes	nulls
T15532	31261-31262	.	denotes	.
T15533	31262-31377	sentence	denotes	In Drosophila, it has been shown that the PHD domain is absolutely necessary for Pygopus function in Wg signaling.
T15534	31263-31265	IN	denotes	In
T15536	31266-31276	NNP	denotes	Drosophila
T15537	31276-31278	,	denotes	,
T15538	31278-31280	PRP	denotes	it
T15539	31281-31284	VBZ	denotes	has
T15540	31285-31289	VBN	denotes	been
T15535	31290-31295	VBN	denotes	shown
T15541	31296-31300	IN	denotes	that
T15543	31301-31304	DT	denotes	the
T15545	31305-31308	NN	denotes	PHD
T15544	31309-31315	NN	denotes	domain
T15542	31316-31318	VBZ	denotes	is
T15546	31319-31329	RB	denotes	absolutely
T15547	31330-31339	JJ	denotes	necessary
T15548	31340-31343	IN	denotes	for
T15549	31344-31351	NN	denotes	Pygopus
T15550	31352-31360	NN	denotes	function
T15551	31361-31363	IN	denotes	in
T15552	31364-31366	NN	denotes	Wg
T15553	31367-31376	NN	denotes	signaling
T15554	31376-31377	.	denotes	.
T15555	31377-31484	sentence	denotes	The PHD domain is 60 amino acids with seven cysteines and a histidine, predicted to chelate two zinc ions.
T15556	31378-31381	DT	denotes	The
T15558	31382-31385	NN	denotes	PHD
T15557	31386-31392	NN	denotes	domain
T15559	31393-31395	VBZ	denotes	is
T15560	31396-31398	CD	denotes	60
T15562	31399-31404	NN	denotes	amino
T15561	31405-31410	NNS	denotes	acids
T15563	31411-31415	IN	denotes	with
T15564	31416-31421	CD	denotes	seven
T15565	31422-31431	NNS	denotes	cysteines
T15566	31432-31435	CC	denotes	and
T15567	31436-31437	DT	denotes	a
T15568	31438-31447	NN	denotes	histidine
T15569	31447-31449	,	denotes	,
T15570	31449-31458	VBN	denotes	predicted
T15571	31459-31461	TO	denotes	to
T15572	31462-31469	VB	denotes	chelate
T15573	31470-31473	CD	denotes	two
T15575	31474-31478	NN	denotes	zinc
T15574	31479-31483	NNS	denotes	ions
T15576	31483-31484	.	denotes	.
T15577	31484-31647	sentence	denotes	PHD domains are found in diverse proteins, including transcription factors, and have been implicated in chromatin remodeling and protein-protein interactions [8].
T15578	31485-31488	NN	denotes	PHD
T15579	31489-31496	NNS	denotes	domains
T15581	31497-31500	VBP	denotes	are
T15580	31501-31506	VBN	denotes	found
T15582	31507-31509	IN	denotes	in
T15583	31510-31517	JJ	denotes	diverse
T15584	31518-31526	NN	denotes	proteins
T15585	31526-31528	,	denotes	,
T15586	31528-31537	VBG	denotes	including
T15587	31538-31551	NN	denotes	transcription
T15588	31552-31559	NNS	denotes	factors
T15589	31559-31561	,	denotes	,
T15590	31561-31564	CC	denotes	and
T15591	31565-31569	VBP	denotes	have
T15593	31570-31574	VBN	denotes	been
T15592	31575-31585	VBN	denotes	implicated
T15594	31586-31588	IN	denotes	in
T15595	31589-31598	NN	denotes	chromatin
T15596	31599-31609	NN	denotes	remodeling
T15597	31610-31613	CC	denotes	and
T15598	31614-31621	NN	denotes	protein
T15600	31621-31622	HYPH	denotes	-
T15599	31622-31629	NN	denotes	protein
T15601	31630-31642	NNS	denotes	interactions
T15602	31643-31644	-LRB-	denotes	[
T15603	31644-31645	CD	denotes	8
T15604	31645-31646	-RRB-	denotes	]
T15605	31646-31647	.	denotes	.
T15606	31647-31871	sentence	denotes	In Drosophila the pygoF107 allele, with a single missense mutation converting amino acid 802 in the PHD domain from cysteine to tyrosine, loses Wnt signaling function in both embryogenesis and imaginal disc development [8].
T15607	31648-31650	IN	denotes	In
T15609	31651-31661	NNP	denotes	Drosophila
T15610	31662-31665	DT	denotes	the
T15612	31666-31674	NN	denotes	pygoF107
T15611	31675-31681	NN	denotes	allele
T15613	31681-31683	,	denotes	,
T15614	31683-31687	IN	denotes	with
T15615	31688-31689	DT	denotes	a
T15617	31690-31696	JJ	denotes	single
T15618	31697-31705	NN	denotes	missense
T15616	31706-31714	NN	denotes	mutation
T15619	31715-31725	VBG	denotes	converting
T15620	31726-31731	NN	denotes	amino
T15621	31732-31736	NN	denotes	acid
T15622	31737-31740	CD	denotes	802
T15623	31741-31743	IN	denotes	in
T15624	31744-31747	DT	denotes	the
T15626	31748-31751	NN	denotes	PHD
T15625	31752-31758	NN	denotes	domain
T15627	31759-31763	IN	denotes	from
T15628	31764-31772	NN	denotes	cysteine
T15629	31773-31775	IN	denotes	to
T15630	31776-31784	NN	denotes	tyrosine
T15631	31784-31786	,	denotes	,
T15608	31786-31791	VBZ	denotes	loses
T15632	31792-31795	NN	denotes	Wnt
T15633	31796-31805	NN	denotes	signaling
T15634	31806-31814	NN	denotes	function
T15635	31815-31817	IN	denotes	in
T15636	31818-31822	CC	denotes	both
T15637	31823-31836	NN	denotes	embryogenesis
T15638	31837-31840	CC	denotes	and
T15639	31841-31849	JJ	denotes	imaginal
T15640	31850-31854	NN	denotes	disc
T15641	31855-31866	NN	denotes	development
T15642	31867-31868	-LRB-	denotes	[
T15643	31868-31869	CD	denotes	8
T15644	31869-31870	-RRB-	denotes	]
T15645	31870-31871	.	denotes	.
T15646	31871-32007	sentence	denotes	The Pygo1/Pygo2 mutant alleles made in this report carried deletions of the entire PHD domains, as well as most other coding sequences.
T15647	31872-31875	DT	denotes	The
T15649	31876-31881	NN	denotes	Pygo1
T15651	31881-31882	HYPH	denotes	/
T15650	31882-31887	NN	denotes	Pygo2
T15652	31888-31894	NN	denotes	mutant
T15648	31895-31902	NNS	denotes	alleles
T15654	31903-31907	VBN	denotes	made
T15655	31908-31910	IN	denotes	in
T15656	31911-31915	DT	denotes	this
T15657	31916-31922	NN	denotes	report
T15653	31923-31930	VBD	denotes	carried
T15658	31931-31940	NNS	denotes	deletions
T15659	31941-31943	IN	denotes	of
T15660	31944-31947	DT	denotes	the
T15662	31948-31954	JJ	denotes	entire
T15663	31955-31958	NN	denotes	PHD
T15661	31959-31966	NNS	denotes	domains
T15664	31966-31968	,	denotes	,
T15665	31968-31970	RB	denotes	as
T15667	31971-31975	RB	denotes	well
T15666	31976-31978	IN	denotes	as
T15668	31979-31983	JJS	denotes	most
T15670	31984-31989	JJ	denotes	other
T15671	31990-31996	NN	denotes	coding
T15669	31997-32006	NNS	denotes	sequences
T15672	32006-32007	.	denotes	.
T15673	32007-32161	sentence	denotes	For the Pygo1 gene, the coding region for 372 of 417 total amino acids was deleted, and for the Pygo2 gene, we deleted coding for 354 of 405 amino acids.
T15674	32008-32011	IN	denotes	For
T15676	32012-32015	DT	denotes	the
T15678	32016-32021	NN	denotes	Pygo1
T15677	32022-32026	NN	denotes	gene
T15679	32026-32028	,	denotes	,
T15680	32028-32031	DT	denotes	the
T15682	32032-32038	NN	denotes	coding
T15681	32039-32045	NN	denotes	region
T15683	32046-32049	IN	denotes	for
T15684	32050-32053	CD	denotes	372
T15686	32054-32056	IN	denotes	of
T15685	32057-32060	CD	denotes	417
T15688	32061-32066	JJ	denotes	total
T15689	32067-32072	NN	denotes	amino
T15687	32073-32078	NNS	denotes	acids
T15690	32079-32082	VBD	denotes	was
T15675	32083-32090	VBN	denotes	deleted
T15691	32090-32092	,	denotes	,
T15692	32092-32095	CC	denotes	and
T15693	32096-32099	IN	denotes	for
T15695	32100-32103	DT	denotes	the
T15697	32104-32109	NN	denotes	Pygo2
T15696	32110-32114	NN	denotes	gene
T15698	32114-32116	,	denotes	,
T15699	32116-32118	PRP	denotes	we
T15694	32119-32126	VBD	denotes	deleted
T15700	32127-32133	VBG	denotes	coding
T15701	32134-32137	IN	denotes	for
T15702	32138-32141	CD	denotes	354
T15704	32142-32144	IN	denotes	of
T15703	32145-32148	CD	denotes	405
T15706	32149-32154	NN	denotes	amino
T15705	32155-32160	NNS	denotes	acids
T15707	32160-32161	.	denotes	.
T15708	32161-32298	sentence	denotes	It is therefore very unlikely that the relatively mild phenotypes observed were the result of residual function of the targeted alleles.
T15709	32162-32164	PRP	denotes	It
T15710	32165-32167	VBZ	denotes	is
T15711	32168-32177	RB	denotes	therefore
T15712	32178-32182	RB	denotes	very
T15713	32183-32191	JJ	denotes	unlikely
T15714	32192-32196	IN	denotes	that
T15716	32197-32200	DT	denotes	the
T15718	32201-32211	RB	denotes	relatively
T15719	32212-32216	JJ	denotes	mild
T15717	32217-32227	NNS	denotes	phenotypes
T15720	32228-32236	VBN	denotes	observed
T15715	32237-32241	VBD	denotes	were
T15721	32242-32245	DT	denotes	the
T15722	32246-32252	NN	denotes	result
T15723	32253-32255	IN	denotes	of
T15724	32256-32264	JJ	denotes	residual
T15725	32265-32273	NN	denotes	function
T15726	32274-32276	IN	denotes	of
T15727	32277-32280	DT	denotes	the
T15729	32281-32289	VBN	denotes	targeted
T15728	32290-32297	NNS	denotes	alleles
T15730	32297-32298	.	denotes	.
T15731	32298-32451	sentence	denotes	We focused our analysis on the developing kidney, in which Wnt signaling has been shown to be of critical importance in several stages of nephrogenesis.
T15732	32299-32301	PRP	denotes	We
T15733	32302-32309	VBD	denotes	focused
T15734	32310-32313	PRP$	denotes	our
T15735	32314-32322	NN	denotes	analysis
T15736	32323-32325	IN	denotes	on
T15737	32326-32329	DT	denotes	the
T15739	32330-32340	VBG	denotes	developing
T15738	32341-32347	NN	denotes	kidney
T15740	32347-32349	,	denotes	,
T15741	32349-32351	IN	denotes	in
T15743	32352-32357	WDT	denotes	which
T15744	32358-32361	NN	denotes	Wnt
T15745	32362-32371	NN	denotes	signaling
T15746	32372-32375	VBZ	denotes	has
T15747	32376-32380	VBN	denotes	been
T15742	32381-32386	VBN	denotes	shown
T15748	32387-32389	TO	denotes	to
T15749	32390-32392	VB	denotes	be
T15750	32393-32395	IN	denotes	of
T15751	32396-32404	JJ	denotes	critical
T15752	32405-32415	NN	denotes	importance
T15753	32416-32418	IN	denotes	in
T15754	32419-32426	JJ	denotes	several
T15755	32427-32433	NNS	denotes	stages
T15756	32434-32436	IN	denotes	of
T15757	32437-32450	NN	denotes	nephrogenesis
T15758	32450-32451	.	denotes	.
T15759	32451-32554	sentence	denotes	Wnt9b is made by the ureteric bud and induces the metanephric mesenchyme to undergo nephrogenesis [2].
T15760	32452-32457	NN	denotes	Wnt9b
T15762	32458-32460	VBZ	denotes	is
T15761	32461-32465	VBN	denotes	made
T15763	32466-32468	IN	denotes	by
T15764	32469-32472	DT	denotes	the
T15766	32473-32481	JJ	denotes	ureteric
T15765	32482-32485	NN	denotes	bud
T15767	32486-32489	CC	denotes	and
T15768	32490-32497	VBZ	denotes	induces
T15769	32498-32501	DT	denotes	the
T15771	32502-32513	JJ	denotes	metanephric
T15770	32514-32524	NN	denotes	mesenchyme
T15772	32525-32527	TO	denotes	to
T15773	32528-32535	VB	denotes	undergo
T15774	32536-32549	NN	denotes	nephrogenesis
T15775	32550-32551	-LRB-	denotes	[
T15776	32551-32552	CD	denotes	2
T15777	32552-32553	-RRB-	denotes	]
T15778	32553-32554	.	denotes	.
T15779	32554-32676	sentence	denotes	Downstream of Wnt9b is Wnt4 [2], which is made by the metanephric mesenchyme, and is also required for nephrogenesis [3].
T15780	32555-32565	RB	denotes	Downstream
T15782	32566-32568	IN	denotes	of
T15783	32569-32574	NN	denotes	Wnt9b
T15781	32575-32577	VBZ	denotes	is
T15784	32578-32582	NN	denotes	Wnt4
T15785	32583-32584	-LRB-	denotes	[
T15786	32584-32585	CD	denotes	2
T15787	32585-32586	-RRB-	denotes	]
T15788	32586-32588	,	denotes	,
T15789	32588-32593	WDT	denotes	which
T15791	32594-32596	VBZ	denotes	is
T15790	32597-32601	VBN	denotes	made
T15792	32602-32604	IN	denotes	by
T15793	32605-32608	DT	denotes	the
T15795	32609-32620	JJ	denotes	metanephric
T15794	32621-32631	NN	denotes	mesenchyme
T15796	32631-32633	,	denotes	,
T15797	32633-32636	CC	denotes	and
T15798	32637-32639	VBZ	denotes	is
T15800	32640-32644	RB	denotes	also
T15799	32645-32653	VBN	denotes	required
T15801	32654-32657	IN	denotes	for
T15802	32658-32671	NN	denotes	nephrogenesis
T15803	32672-32673	-LRB-	denotes	[
T15804	32673-32674	CD	denotes	3
T15805	32674-32675	-RRB-	denotes	]
T15806	32675-32676	.	denotes	.
T15807	32676-32795	sentence	denotes	In addition, Wnt11 is produced by the ureteric bud tips and induces GDNF expression in the metanephric mesenchyme [4].
T15808	32677-32679	IN	denotes	In
T15810	32680-32688	NN	denotes	addition
T15811	32688-32690	,	denotes	,
T15812	32690-32695	NN	denotes	Wnt11
T15813	32696-32698	VBZ	denotes	is
T15809	32699-32707	VBN	denotes	produced
T15814	32708-32710	IN	denotes	by
T15815	32711-32714	DT	denotes	the
T15817	32715-32723	JJ	denotes	ureteric
T15818	32724-32727	NN	denotes	bud
T15816	32728-32732	NNS	denotes	tips
T15819	32733-32736	CC	denotes	and
T15820	32737-32744	VBZ	denotes	induces
T15821	32745-32749	NN	denotes	GDNF
T15822	32750-32760	NN	denotes	expression
T15823	32761-32763	IN	denotes	in
T15824	32764-32767	DT	denotes	the
T15826	32768-32779	JJ	denotes	metanephric
T15825	32780-32790	NN	denotes	mesenchyme
T15827	32791-32792	-LRB-	denotes	[
T15828	32792-32793	CD	denotes	4
T15829	32793-32794	-RRB-	denotes	]
T15830	32794-32795	.	denotes	.
T15831	32795-32866	sentence	denotes	In this report we describe a novel Wnt function in kidney development.
T15832	32796-32798	IN	denotes	In
T15834	32799-32803	DT	denotes	this
T15835	32804-32810	NN	denotes	report
T15836	32811-32813	PRP	denotes	we
T15833	32814-32822	VBP	denotes	describe
T15837	32823-32824	DT	denotes	a
T15839	32825-32830	JJ	denotes	novel
T15840	32831-32834	NN	denotes	Wnt
T15838	32835-32843	NN	denotes	function
T15841	32844-32846	IN	denotes	in
T15842	32847-32853	NN	denotes	kidney
T15843	32854-32865	NN	denotes	development
T15844	32865-32866	.	denotes	.
T15845	32866-33013	sentence	denotes	The BAT-gal transgene reporter indicated the presence of canonical Wnt signaling in the ureteric bud and its derivatives in the developing kidney.
T15846	32867-32870	DT	denotes	The
T15848	32871-32874	NN	denotes	BAT
T15850	32874-32875	HYPH	denotes	-
T15849	32875-32878	NN	denotes	gal
T15851	32879-32888	NN	denotes	transgene
T15847	32889-32897	NN	denotes	reporter
T15852	32898-32907	VBD	denotes	indicated
T15853	32908-32911	DT	denotes	the
T15854	32912-32920	NN	denotes	presence
T15855	32921-32923	IN	denotes	of
T15856	32924-32933	JJ	denotes	canonical
T15858	32934-32937	NN	denotes	Wnt
T15857	32938-32947	NN	denotes	signaling
T15859	32948-32950	IN	denotes	in
T15860	32951-32954	DT	denotes	the
T15862	32955-32963	JJ	denotes	ureteric
T15861	32964-32967	NN	denotes	bud
T15863	32968-32971	CC	denotes	and
T15864	32972-32975	PRP$	denotes	its
T15865	32976-32987	NNS	denotes	derivatives
T15866	32988-32990	IN	denotes	in
T15867	32991-32994	DT	denotes	the
T15869	32995-33005	VBG	denotes	developing
T15868	33006-33012	NN	denotes	kidney
T15870	33012-33013	.	denotes	.
T15871	33013-33116	sentence	denotes	Further, in the Pygo2 mutants this signal was lost, suggesting significant reduction of Wnt signaling.
T15872	33014-33021	RB	denotes	Further
T15874	33021-33023	,	denotes	,
T15875	33023-33025	IN	denotes	in
T15876	33026-33029	DT	denotes	the
T15878	33030-33035	NN	denotes	Pygo2
T15877	33036-33043	NNS	denotes	mutants
T15879	33044-33048	DT	denotes	this
T15880	33049-33055	NN	denotes	signal
T15881	33056-33059	VBD	denotes	was
T15873	33060-33064	VBN	denotes	lost
T15882	33064-33066	,	denotes	,
T15883	33066-33076	VBG	denotes	suggesting
T15884	33077-33088	JJ	denotes	significant
T15885	33089-33098	NN	denotes	reduction
T15886	33099-33101	IN	denotes	of
T15887	33102-33105	NN	denotes	Wnt
T15888	33106-33115	NN	denotes	signaling
T15889	33115-33116	.	denotes	.
T15890	33116-33357	sentence	denotes	In addition, we observed a resulting decrease in ureteric tip density, reduced kidney size and altered morphology of the ureteric tree in mutants, indicating a role for canonical Wnt signaling in branching morphogenesis of the ureteric bud.
T15891	33117-33119	IN	denotes	In
T15893	33120-33128	NN	denotes	addition
T15894	33128-33130	,	denotes	,
T15895	33130-33132	PRP	denotes	we
T15892	33133-33141	VBD	denotes	observed
T15896	33142-33143	DT	denotes	a
T15898	33144-33153	VBG	denotes	resulting
T15897	33154-33162	NN	denotes	decrease
T15899	33163-33165	IN	denotes	in
T15900	33166-33174	JJ	denotes	ureteric
T15901	33175-33178	NN	denotes	tip
T15902	33179-33186	NN	denotes	density
T15903	33186-33188	,	denotes	,
T15904	33188-33195	VBN	denotes	reduced
T15906	33196-33202	NN	denotes	kidney
T15905	33203-33207	NN	denotes	size
T15907	33208-33211	CC	denotes	and
T15908	33212-33219	VBN	denotes	altered
T15909	33220-33230	NN	denotes	morphology
T15910	33231-33233	IN	denotes	of
T15911	33234-33237	DT	denotes	the
T15913	33238-33246	JJ	denotes	ureteric
T15912	33247-33251	NN	denotes	tree
T15914	33252-33254	IN	denotes	in
T15915	33255-33262	NNS	denotes	mutants
T15916	33262-33264	,	denotes	,
T15917	33264-33274	VBG	denotes	indicating
T15918	33275-33276	DT	denotes	a
T15919	33277-33281	NN	denotes	role
T15920	33282-33285	IN	denotes	for
T15921	33286-33295	JJ	denotes	canonical
T15923	33296-33299	NN	denotes	Wnt
T15922	33300-33309	NN	denotes	signaling
T15924	33310-33312	IN	denotes	in
T15925	33313-33322	VBG	denotes	branching
T15926	33323-33336	NN	denotes	morphogenesis
T15927	33337-33339	IN	denotes	of
T15928	33340-33343	DT	denotes	the
T15930	33344-33352	JJ	denotes	ureteric
T15929	33353-33356	NN	denotes	bud
T15931	33356-33357	.	denotes	.
T15932	33357-33634	sentence	denotes	While the simplest interpretation is direct Wnt signaling to the ureteric bud, it remains possible that the observed abnormalities are the result of indirect effects, with altered Wnt signaling to the metanephric mesenchyme then affecting mesenchyme to ureteric bud signaling.
T15933	33358-33363	IN	denotes	While
T15935	33364-33367	DT	denotes	the
T15937	33368-33376	JJS	denotes	simplest
T15936	33377-33391	NN	denotes	interpretation
T15934	33392-33394	VBZ	denotes	is
T15939	33395-33401	JJ	denotes	direct
T15941	33402-33405	NN	denotes	Wnt
T15940	33406-33415	NN	denotes	signaling
T15942	33416-33418	IN	denotes	to
T15943	33419-33422	DT	denotes	the
T15945	33423-33431	JJ	denotes	ureteric
T15944	33432-33435	NN	denotes	bud
T15946	33435-33437	,	denotes	,
T15947	33437-33439	PRP	denotes	it
T15938	33440-33447	VBZ	denotes	remains
T15948	33448-33456	JJ	denotes	possible
T15949	33457-33461	IN	denotes	that
T15951	33462-33465	DT	denotes	the
T15953	33466-33474	VBN	denotes	observed
T15952	33475-33488	NNS	denotes	abnormalities
T15950	33489-33492	VBP	denotes	are
T15954	33493-33496	DT	denotes	the
T15955	33497-33503	NN	denotes	result
T15956	33504-33506	IN	denotes	of
T15957	33507-33515	JJ	denotes	indirect
T15958	33516-33523	NNS	denotes	effects
T15959	33523-33525	,	denotes	,
T15960	33525-33529	IN	denotes	with
T15961	33530-33537	VBN	denotes	altered
T15963	33538-33541	NN	denotes	Wnt
T15962	33542-33551	NN	denotes	signaling
T15965	33552-33554	IN	denotes	to
T15966	33555-33558	DT	denotes	the
T15968	33559-33570	JJ	denotes	metanephric
T15967	33571-33581	NN	denotes	mesenchyme
T15969	33582-33586	RB	denotes	then
T15964	33587-33596	VBG	denotes	affecting
T15970	33597-33607	NN	denotes	mesenchyme
T15971	33608-33610	IN	denotes	to
T15972	33611-33619	JJ	denotes	ureteric
T15973	33620-33623	NN	denotes	bud
T15974	33624-33633	NN	denotes	signaling
T15975	33633-33634	.	denotes	.
T15976	33634-33797	sentence	denotes	The Pygo1/Pygo2 mutant phenotype of reduced branching morphogenesis of the ureteric bud is surprisingly similar to that previously reported for Wnt11 mutants [4].
T15977	33635-33638	DT	denotes	The
T15979	33639-33644	NN	denotes	Pygo1
T15981	33644-33645	HYPH	denotes	/
T15980	33645-33650	NN	denotes	Pygo2
T15982	33651-33657	NN	denotes	mutant
T15978	33658-33667	NN	denotes	phenotype
T15984	33668-33670	IN	denotes	of
T15985	33671-33678	VBN	denotes	reduced
T15987	33679-33688	NN	denotes	branching
T15986	33689-33702	NN	denotes	morphogenesis
T15988	33703-33705	IN	denotes	of
T15989	33706-33709	DT	denotes	the
T15991	33710-33718	JJ	denotes	ureteric
T15990	33719-33722	NN	denotes	bud
T15983	33723-33725	VBZ	denotes	is
T15992	33726-33738	RB	denotes	surprisingly
T15993	33739-33746	JJ	denotes	similar
T15994	33747-33749	IN	denotes	to
T15995	33750-33754	DT	denotes	that
T15996	33755-33765	RB	denotes	previously
T15997	33766-33774	VBN	denotes	reported
T15998	33775-33778	IN	denotes	for
T15999	33779-33784	NN	denotes	Wnt11
T16000	33785-33792	NNS	denotes	mutants
T16001	33793-33794	-LRB-	denotes	[
T16002	33794-33795	CD	denotes	4
T16003	33795-33796	-RRB-	denotes	]
T16004	33796-33797	.	denotes	.
T16005	33797-33860	sentence	denotes	The underlying mechanisms, however, are likely to be distinct.
T16006	33798-33801	DT	denotes	The
T16008	33802-33812	VBG	denotes	underlying
T16007	33813-33823	NNS	denotes	mechanisms
T16010	33823-33825	,	denotes	,
T16011	33825-33832	RB	denotes	however
T16012	33832-33834	,	denotes	,
T16009	33834-33837	VBP	denotes	are
T16013	33838-33844	JJ	denotes	likely
T16014	33845-33847	TO	denotes	to
T16015	33848-33850	VB	denotes	be
T16016	33851-33859	JJ	denotes	distinct
T16017	33859-33860	.	denotes	.
T16018	33860-34029	sentence	denotes	Wnt11 is generally thought to act through a noncanonical pathway, (although exceptions have been noted [31]), whereas the Pygopus genes promote canonical Wnt signaling.
T16019	33861-33866	NN	denotes	Wnt11
T16021	33867-33869	VBZ	denotes	is
T16022	33870-33879	RB	denotes	generally
T16020	33880-33887	VBN	denotes	thought
T16023	33888-33890	TO	denotes	to
T16024	33891-33894	VB	denotes	act
T16025	33895-33902	IN	denotes	through
T16026	33903-33904	DT	denotes	a
T16028	33905-33917	JJ	denotes	noncanonical
T16027	33918-33925	NN	denotes	pathway
T16029	33925-33927	,	denotes	,
T16030	33927-33928	-LRB-	denotes	(
T16031	33928-33936	IN	denotes	although
T16033	33937-33947	NNS	denotes	exceptions
T16034	33948-33952	VBP	denotes	have
T16035	33953-33957	VBN	denotes	been
T16032	33958-33963	VBN	denotes	noted
T16036	33964-33965	-LRB-	denotes	[
T16037	33965-33967	CD	denotes	31
T16038	33967-33968	-RRB-	denotes	]
T16039	33968-33969	-RRB-	denotes	)
T16040	33969-33971	,	denotes	,
T16041	33971-33978	IN	denotes	whereas
T16043	33979-33982	DT	denotes	the
T16045	33983-33990	NN	denotes	Pygopus
T16044	33991-33996	NNS	denotes	genes
T16042	33997-34004	VBP	denotes	promote
T16046	34005-34014	JJ	denotes	canonical
T16048	34015-34018	NN	denotes	Wnt
T16047	34019-34028	NN	denotes	signaling
T16049	34028-34029	.	denotes	.
T16050	34029-34294	sentence	denotes	Further, the Wnt11 mutants showed an altered feedback loop between Wnt signaling from the ureteric bud to the mesenchyme and GDNF signaling from the mesenchyme to the bud, whereas in the Pygo1/Pygo2 mutants, we observed disrupted Wnt signaling in the ureteric bud.
T16051	34030-34037	RB	denotes	Further
T16053	34037-34039	,	denotes	,
T16054	34039-34042	DT	denotes	the
T16056	34043-34048	NN	denotes	Wnt11
T16055	34049-34056	NNS	denotes	mutants
T16052	34057-34063	VBD	denotes	showed
T16057	34064-34066	DT	denotes	an
T16059	34067-34074	VBN	denotes	altered
T16060	34075-34083	NN	denotes	feedback
T16058	34084-34088	NN	denotes	loop
T16061	34089-34096	IN	denotes	between
T16062	34097-34100	NN	denotes	Wnt
T16063	34101-34110	NN	denotes	signaling
T16064	34111-34115	IN	denotes	from
T16065	34116-34119	DT	denotes	the
T16067	34120-34128	JJ	denotes	ureteric
T16066	34129-34132	NN	denotes	bud
T16068	34133-34135	IN	denotes	to
T16069	34136-34139	DT	denotes	the
T16070	34140-34150	NN	denotes	mesenchyme
T16071	34151-34154	CC	denotes	and
T16072	34155-34159	NN	denotes	GDNF
T16073	34160-34169	NN	denotes	signaling
T16074	34170-34174	IN	denotes	from
T16075	34175-34178	DT	denotes	the
T16076	34179-34189	NN	denotes	mesenchyme
T16077	34190-34192	IN	denotes	to
T16078	34193-34196	DT	denotes	the
T16079	34197-34200	NN	denotes	bud
T16080	34200-34202	,	denotes	,
T16081	34202-34209	IN	denotes	whereas
T16083	34210-34212	IN	denotes	in
T16084	34213-34216	DT	denotes	the
T16086	34217-34222	NN	denotes	Pygo1
T16088	34222-34223	HYPH	denotes	/
T16087	34223-34228	NN	denotes	Pygo2
T16085	34229-34236	NNS	denotes	mutants
T16089	34236-34238	,	denotes	,
T16090	34238-34240	PRP	denotes	we
T16082	34241-34249	VBD	denotes	observed
T16091	34250-34259	VBN	denotes	disrupted
T16093	34260-34263	NN	denotes	Wnt
T16092	34264-34273	NN	denotes	signaling
T16094	34274-34276	IN	denotes	in
T16095	34277-34280	DT	denotes	the
T16097	34281-34289	JJ	denotes	ureteric
T16096	34290-34293	NN	denotes	bud
T16098	34293-34294	.	denotes	.
T16099	34294-34403	sentence	denotes	The Pygo1/2 mutants also showed an expansion of the zone of thickened mesenchyme that caps the ureteric bud.
T16100	34295-34298	DT	denotes	The
T16102	34299-34304	NN	denotes	Pygo1
T16103	34304-34305	HYPH	denotes	/
T16104	34305-34306	CD	denotes	2
T16101	34307-34314	NNS	denotes	mutants
T16106	34315-34319	RB	denotes	also
T16105	34320-34326	VBD	denotes	showed
T16107	34327-34329	DT	denotes	an
T16108	34330-34339	NN	denotes	expansion
T16109	34340-34342	IN	denotes	of
T16110	34343-34346	DT	denotes	the
T16111	34347-34351	NN	denotes	zone
T16112	34352-34354	IN	denotes	of
T16113	34355-34364	VBN	denotes	thickened
T16114	34365-34375	NN	denotes	mesenchyme
T16115	34376-34380	WDT	denotes	that
T16116	34381-34385	VBZ	denotes	caps
T16117	34386-34389	DT	denotes	the
T16119	34390-34398	JJ	denotes	ureteric
T16118	34399-34402	NN	denotes	bud
T16120	34402-34403	.	denotes	.
T16121	34403-34477	sentence	denotes	Nevertheless, the mutant metanephric mesenchyme formed nephrons normally.
T16122	34404-34416	RB	denotes	Nevertheless
T16124	34416-34418	,	denotes	,
T16125	34418-34421	DT	denotes	the
T16127	34422-34428	NN	denotes	mutant
T16128	34429-34440	JJ	denotes	metanephric
T16126	34441-34451	NN	denotes	mesenchyme
T16123	34452-34458	VBD	denotes	formed
T16129	34459-34467	NNS	denotes	nephrons
T16130	34468-34476	RB	denotes	normally
T16131	34476-34477	.	denotes	.
T16132	34477-34655	sentence	denotes	This was particularly interesting, as Wnt9b signaling from the ureteric bud to the metanephric mesenchyme has been shown to induce nephrogenesis via canonical Wnt signaling [2].
T16133	34478-34482	DT	denotes	This
T16134	34483-34486	VBD	denotes	was
T16135	34487-34499	RB	denotes	particularly
T16136	34500-34511	JJ	denotes	interesting
T16137	34511-34513	,	denotes	,
T16138	34513-34515	IN	denotes	as
T16140	34516-34521	NN	denotes	Wnt9b
T16141	34522-34531	NN	denotes	signaling
T16142	34532-34536	IN	denotes	from
T16143	34537-34540	DT	denotes	the
T16145	34541-34549	JJ	denotes	ureteric
T16144	34550-34553	NN	denotes	bud
T16146	34554-34556	IN	denotes	to
T16147	34557-34560	DT	denotes	the
T16149	34561-34572	JJ	denotes	metanephric
T16148	34573-34583	NN	denotes	mesenchyme
T16150	34584-34587	VBZ	denotes	has
T16151	34588-34592	VBN	denotes	been
T16139	34593-34598	VBN	denotes	shown
T16152	34599-34601	TO	denotes	to
T16153	34602-34608	VB	denotes	induce
T16154	34609-34622	NN	denotes	nephrogenesis
T16155	34623-34626	IN	denotes	via
T16156	34627-34636	JJ	denotes	canonical
T16158	34637-34640	NN	denotes	Wnt
T16157	34641-34650	NN	denotes	signaling
T16159	34651-34652	-LRB-	denotes	[
T16160	34652-34653	CD	denotes	2
T16161	34653-34654	-RRB-	denotes	]
T16162	34654-34655	.	denotes	.
T16163	34655-34793	sentence	denotes	The results in this report indicate a striking and surprising evolutionary divergence of Pygopus function between Drosophila and mammals.
T16164	34656-34659	DT	denotes	The
T16165	34660-34667	NNS	denotes	results
T16167	34668-34670	IN	denotes	in
T16168	34671-34675	DT	denotes	this
T16169	34676-34682	NN	denotes	report
T16166	34683-34691	VBP	denotes	indicate
T16170	34692-34693	DT	denotes	a
T16172	34694-34702	JJ	denotes	striking
T16173	34703-34706	CC	denotes	and
T16174	34707-34717	JJ	denotes	surprising
T16175	34718-34730	JJ	denotes	evolutionary
T16171	34731-34741	NN	denotes	divergence
T16176	34742-34744	IN	denotes	of
T16177	34745-34752	NN	denotes	Pygopus
T16178	34753-34761	NN	denotes	function
T16179	34762-34769	IN	denotes	between
T16180	34770-34780	NNP	denotes	Drosophila
T16181	34781-34784	CC	denotes	and
T16182	34785-34792	NNS	denotes	mammals
T16183	34792-34793	.	denotes	.
T16184	34793-34969	sentence	denotes	In Drosophila, the Pygopus gene is often required for canonical Wnt signaling, while in mammals the Pygo1/Pygo2 genes appear to play a smaller role in canonical Wnt signaling.
T16185	34794-34796	IN	denotes	In
T16187	34797-34807	NNP	denotes	Drosophila
T16188	34807-34809	,	denotes	,
T16189	34809-34812	DT	denotes	the
T16191	34813-34820	NN	denotes	Pygopus
T16190	34821-34825	NN	denotes	gene
T16192	34826-34828	VBZ	denotes	is
T16193	34829-34834	RB	denotes	often
T16186	34835-34843	VBN	denotes	required
T16194	34844-34847	IN	denotes	for
T16195	34848-34857	JJ	denotes	canonical
T16197	34858-34861	NN	denotes	Wnt
T16196	34862-34871	NN	denotes	signaling
T16198	34871-34873	,	denotes	,
T16199	34873-34878	IN	denotes	while
T16201	34879-34881	IN	denotes	in
T16202	34882-34889	NNS	denotes	mammals
T16203	34890-34893	DT	denotes	the
T16205	34894-34899	NN	denotes	Pygo1
T16207	34899-34900	HYPH	denotes	/
T16206	34900-34905	NN	denotes	Pygo2
T16204	34906-34911	NNS	denotes	genes
T16200	34912-34918	VBP	denotes	appear
T16208	34919-34921	TO	denotes	to
T16209	34922-34926	VB	denotes	play
T16210	34927-34928	DT	denotes	a
T16212	34929-34936	JJR	denotes	smaller
T16211	34937-34941	NN	denotes	role
T16213	34942-34944	IN	denotes	in
T16214	34945-34954	JJ	denotes	canonical
T16216	34955-34958	NN	denotes	Wnt
T16215	34959-34968	NN	denotes	signaling
T16217	34968-34969	.	denotes	.
T16218	34969-35160	sentence	denotes	The BAT-gal transgene reporter of canonical Wnt signaling showed reduced but generally not absent signal in Pygo1/Pygo2 mutant embryos, with tissue-specific variation in level of diminution.
T16219	34970-34973	DT	denotes	The
T16221	34974-34977	NN	denotes	BAT
T16223	34977-34978	HYPH	denotes	-
T16222	34978-34981	NN	denotes	gal
T16224	34982-34991	NN	denotes	transgene
T16220	34992-35000	NN	denotes	reporter
T16226	35001-35003	IN	denotes	of
T16227	35004-35013	JJ	denotes	canonical
T16229	35014-35017	NN	denotes	Wnt
T16228	35018-35027	NN	denotes	signaling
T16225	35028-35034	VBD	denotes	showed
T16230	35035-35042	VBN	denotes	reduced
T16232	35043-35046	CC	denotes	but
T16233	35047-35056	RB	denotes	generally
T16234	35057-35060	RB	denotes	not
T16235	35061-35067	JJ	denotes	absent
T16231	35068-35074	NN	denotes	signal
T16236	35075-35077	IN	denotes	in
T16237	35078-35083	NN	denotes	Pygo1
T16239	35083-35084	HYPH	denotes	/
T16238	35084-35089	NN	denotes	Pygo2
T16241	35090-35096	NN	denotes	mutant
T16240	35097-35104	NNS	denotes	embryos
T16242	35104-35106	,	denotes	,
T16243	35106-35110	IN	denotes	with
T16244	35111-35117	NN	denotes	tissue
T16246	35117-35118	HYPH	denotes	-
T16245	35118-35126	JJ	denotes	specific
T16247	35127-35136	NN	denotes	variation
T16248	35137-35139	IN	denotes	in
T16249	35140-35145	NN	denotes	level
T16250	35146-35148	IN	denotes	of
T16251	35149-35159	NN	denotes	diminution
T16252	35159-35160	.	denotes	.
T16253	35160-35268	sentence	denotes	In addition, the kidneys were not unique in showing surprisingly normal development in Pygo1/Pygo2 mutants.
T16254	35161-35163	IN	denotes	In
T16256	35164-35172	NN	denotes	addition
T16257	35172-35174	,	denotes	,
T16258	35174-35177	DT	denotes	the
T16259	35178-35185	NNS	denotes	kidneys
T16255	35186-35190	VBD	denotes	were
T16260	35191-35194	RB	denotes	not
T16261	35195-35201	JJ	denotes	unique
T16262	35202-35204	IN	denotes	in
T16263	35205-35212	VBG	denotes	showing
T16264	35213-35225	RB	denotes	surprisingly
T16265	35226-35232	JJ	denotes	normal
T16266	35233-35244	NN	denotes	development
T16267	35245-35247	IN	denotes	in
T16268	35248-35253	NN	denotes	Pygo1
T16270	35253-35254	HYPH	denotes	/
T16269	35254-35259	NN	denotes	Pygo2
T16271	35260-35267	NNS	denotes	mutants
T16272	35267-35268	.	denotes	.
T16273	35268-35363	sentence	denotes	Indeed, organogenesis generally proceeded without detectable abnormality, with few exceptions.
T16274	35269-35275	RB	denotes	Indeed
T16276	35275-35277	,	denotes	,
T16277	35277-35290	NN	denotes	organogenesis
T16278	35291-35300	RB	denotes	generally
T16275	35301-35310	VBD	denotes	proceeded
T16279	35311-35318	IN	denotes	without
T16280	35319-35329	JJ	denotes	detectable
T16281	35330-35341	NN	denotes	abnormality
T16282	35341-35343	,	denotes	,
T16283	35343-35347	IN	denotes	with
T16284	35348-35351	JJ	denotes	few
T16285	35352-35362	NNS	denotes	exceptions
T16286	35362-35363	.	denotes	.
T16287	35363-35532	sentence	denotes	These results suggest that the proteins encoded by mammalian Pygopus genes are often mere modulators of canonical Wnt signaling intensity, and not essential components.
T16288	35364-35369	DT	denotes	These
T16289	35370-35377	NNS	denotes	results
T16290	35378-35385	VBP	denotes	suggest
T16291	35386-35390	IN	denotes	that
T16293	35391-35394	DT	denotes	the
T16294	35395-35403	NN	denotes	proteins
T16295	35404-35411	VBN	denotes	encoded
T16296	35412-35414	IN	denotes	by
T16297	35415-35424	JJ	denotes	mammalian
T16299	35425-35432	NN	denotes	Pygopus
T16298	35433-35438	NNS	denotes	genes
T16292	35439-35442	VBP	denotes	are
T16300	35443-35448	RB	denotes	often
T16301	35449-35453	JJ	denotes	mere
T16302	35454-35464	NNS	denotes	modulators
T16303	35465-35467	IN	denotes	of
T16304	35468-35477	JJ	denotes	canonical
T16306	35478-35481	NN	denotes	Wnt
T16305	35482-35491	NN	denotes	signaling
T16307	35492-35501	NN	denotes	intensity
T16308	35501-35503	,	denotes	,
T16309	35503-35506	CC	denotes	and
T16310	35507-35510	RB	denotes	not
T16311	35511-35520	JJ	denotes	essential
T16312	35521-35531	NNS	denotes	components
T16313	35531-35532	.	denotes	.
T16314	35532-35588	sentence	denotes	The microarray results further support this conclusion.
T16315	35533-35536	DT	denotes	The
T16317	35537-35547	NN	denotes	microarray
T16316	35548-35555	NNS	denotes	results
T16319	35556-35563	RB	denotes	further
T16318	35564-35571	VBZ	denotes	support
T16320	35572-35576	DT	denotes	this
T16321	35577-35587	NN	denotes	conclusion
T16322	35587-35588	.	denotes	.
T16323	35588-35779	sentence	denotes	Whereas the BAT-gal transgene reporter monitors the expression level of only one Wnt responsive promoter, the microarray allows the analysis of the activity levels of promoters of all genes.
T16324	35589-35596	IN	denotes	Whereas
T16326	35597-35600	DT	denotes	the
T16328	35601-35604	NN	denotes	BAT
T16330	35604-35605	HYPH	denotes	-
T16329	35605-35608	NN	denotes	gal
T16331	35609-35618	NN	denotes	transgene
T16327	35619-35627	NN	denotes	reporter
T16325	35628-35636	VBZ	denotes	monitors
T16333	35637-35640	DT	denotes	the
T16335	35641-35651	NN	denotes	expression
T16334	35652-35657	NN	denotes	level
T16336	35658-35660	IN	denotes	of
T16337	35661-35665	RB	denotes	only
T16338	35666-35669	CD	denotes	one
T16340	35670-35673	NN	denotes	Wnt
T16341	35674-35684	JJ	denotes	responsive
T16339	35685-35693	NN	denotes	promoter
T16342	35693-35695	,	denotes	,
T16343	35695-35698	DT	denotes	the
T16344	35699-35709	NN	denotes	microarray
T16332	35710-35716	VBZ	denotes	allows
T16345	35717-35720	DT	denotes	the
T16346	35721-35729	NN	denotes	analysis
T16347	35730-35732	IN	denotes	of
T16348	35733-35736	DT	denotes	the
T16350	35737-35745	NN	denotes	activity
T16349	35746-35752	NNS	denotes	levels
T16351	35753-35755	IN	denotes	of
T16352	35756-35765	NNS	denotes	promoters
T16353	35766-35768	IN	denotes	of
T16354	35769-35772	DT	denotes	all
T16355	35773-35778	NNS	denotes	genes
T16356	35778-35779	.	denotes	.
T16357	35779-35880	sentence	denotes	Interestingly, the mutant kidneys showed gene-expression profiles surprisingly similar to wild type.
T16358	35780-35793	RB	denotes	Interestingly
T16360	35793-35795	,	denotes	,
T16361	35795-35798	DT	denotes	the
T16363	35799-35805	NN	denotes	mutant
T16362	35806-35813	NNS	denotes	kidneys
T16359	35814-35820	VBD	denotes	showed
T16364	35821-35825	NN	denotes	gene
T16366	35825-35826	HYPH	denotes	-
T16365	35826-35836	NN	denotes	expression
T16367	35837-35845	NNS	denotes	profiles
T16368	35846-35858	RB	denotes	surprisingly
T16369	35859-35866	JJ	denotes	similar
T16370	35867-35869	IN	denotes	to
T16371	35870-35874	JJ	denotes	wild
T16372	35875-35879	NN	denotes	type
T16373	35879-35880	.	denotes	.
T16374	35880-36050	sentence	denotes	Some genes did, however, show expression differences, and a high percentage of these differences could be validated by real-time PCR with independent biological samples.
T16375	35881-35885	DT	denotes	Some
T16376	35886-35891	NNS	denotes	genes
T16378	35892-35895	VBD	denotes	did
T16379	35895-35897	,	denotes	,
T16380	35897-35904	RB	denotes	however
T16381	35904-35906	,	denotes	,
T16377	35906-35910	VB	denotes	show
T16382	35911-35921	NN	denotes	expression
T16383	35922-35933	NNS	denotes	differences
T16384	35933-35935	,	denotes	,
T16385	35935-35938	CC	denotes	and
T16386	35939-35940	DT	denotes	a
T16388	35941-35945	JJ	denotes	high
T16387	35946-35956	NN	denotes	percentage
T16390	35957-35959	IN	denotes	of
T16391	35960-35965	DT	denotes	these
T16392	35966-35977	NNS	denotes	differences
T16393	35978-35983	MD	denotes	could
T16394	35984-35986	VB	denotes	be
T16389	35987-35996	VBN	denotes	validated
T16395	35997-35999	IN	denotes	by
T16396	36000-36004	JJ	denotes	real
T16398	36004-36005	HYPH	denotes	-
T16397	36005-36009	NN	denotes	time
T16399	36010-36013	NN	denotes	PCR
T16400	36014-36018	IN	denotes	with
T16401	36019-36030	JJ	denotes	independent
T16403	36031-36041	JJ	denotes	biological
T16402	36042-36049	NNS	denotes	samples
T16404	36049-36050	.	denotes	.
T16405	36050-36293	sentence	denotes	Assuming that the Pygopus genes in mammals are dedicated to canonical Wnt signaling, as has been previously shown in Drosophila, the genes with expression differences represent candidate Wnt targets (direct or indirect) in kidney development.
T16406	36051-36059	VBG	denotes	Assuming
T16408	36060-36064	IN	denotes	that
T16410	36065-36068	DT	denotes	the
T16412	36069-36076	NN	denotes	Pygopus
T16411	36077-36082	NNS	denotes	genes
T16413	36083-36085	IN	denotes	in
T16414	36086-36093	NNS	denotes	mammals
T16415	36094-36097	VBP	denotes	are
T16409	36098-36107	VBN	denotes	dedicated
T16416	36108-36110	IN	denotes	to
T16417	36111-36120	JJ	denotes	canonical
T16419	36121-36124	NN	denotes	Wnt
T16418	36125-36134	NN	denotes	signaling
T16420	36134-36136	,	denotes	,
T16421	36136-36138	IN	denotes	as
T16423	36139-36142	VBZ	denotes	has
T16424	36143-36147	VBN	denotes	been
T16425	36148-36158	RB	denotes	previously
T16422	36159-36164	VBN	denotes	shown
T16426	36165-36167	IN	denotes	in
T16427	36168-36178	NNP	denotes	Drosophila
T16428	36178-36180	,	denotes	,
T16429	36180-36183	DT	denotes	the
T16430	36184-36189	NNS	denotes	genes
T16431	36190-36194	IN	denotes	with
T16432	36195-36205	NN	denotes	expression
T16433	36206-36217	NNS	denotes	differences
T16407	36218-36227	VBP	denotes	represent
T16434	36228-36237	NN	denotes	candidate
T16436	36238-36241	NN	denotes	Wnt
T16435	36242-36249	NNS	denotes	targets
T16437	36250-36251	-LRB-	denotes	(
T16438	36251-36257	JJ	denotes	direct
T16439	36258-36260	CC	denotes	or
T16440	36261-36269	JJ	denotes	indirect
T16441	36269-36270	-RRB-	denotes	)
T16442	36271-36273	IN	denotes	in
T16443	36274-36280	NN	denotes	kidney
T16444	36281-36292	NN	denotes	development
T16445	36292-36293	.	denotes	.
T16446	36293-36446	sentence	denotes	We would predict these genes to show greater changes in expression level in a developing kidney with a more complete removal of canonical Wnt signaling.
T16447	36294-36296	PRP	denotes	We
T16449	36297-36302	MD	denotes	would
T16448	36303-36310	VB	denotes	predict
T16450	36311-36316	DT	denotes	these
T16451	36317-36322	NNS	denotes	genes
T16452	36323-36325	TO	denotes	to
T16453	36326-36330	VB	denotes	show
T16454	36331-36338	JJR	denotes	greater
T16455	36339-36346	NNS	denotes	changes
T16456	36347-36349	IN	denotes	in
T16457	36350-36360	NN	denotes	expression
T16458	36361-36366	NN	denotes	level
T16459	36367-36369	IN	denotes	in
T16460	36370-36371	DT	denotes	a
T16462	36372-36382	VBG	denotes	developing
T16461	36383-36389	NN	denotes	kidney
T16463	36390-36394	IN	denotes	with
T16464	36395-36396	DT	denotes	a
T16466	36397-36401	RBR	denotes	more
T16467	36402-36410	JJ	denotes	complete
T16465	36411-36418	NN	denotes	removal
T16468	36419-36421	IN	denotes	of
T16469	36422-36431	JJ	denotes	canonical
T16471	36432-36435	NN	denotes	Wnt
T16470	36436-36445	NN	denotes	signaling
T16472	36445-36446	.	denotes	.
T17129	36459-36461	IN	denotes	In
T17131	36462-36472	NN	denotes	conclusion
T17132	36472-36474	,	denotes	,
T17133	36474-36477	DT	denotes	the
T17135	36478-36487	JJ	denotes	mammalian
T17136	36488-36493	NN	denotes	Pygo2
T17134	36494-36498	NN	denotes	gene
T17137	36499-36501	VBZ	denotes	is
T17130	36502-36510	VBN	denotes	required
T17138	36511-36514	IN	denotes	for
T17139	36515-36521	JJ	denotes	normal
T17141	36522-36531	NN	denotes	branching
T17140	36532-36545	NN	denotes	morphogenesis
T17142	36546-36548	IN	denotes	of
T17143	36549-36552	DT	denotes	the
T17145	36553-36561	JJ	denotes	ureteric
T17144	36562-36565	NN	denotes	bud
T17146	36565-36567	,	denotes	,
T17147	36567-36571	IN	denotes	with
T17148	36572-36579	NNS	denotes	mutants
T17149	36580-36587	VBG	denotes	showing
T17150	36588-36595	VBN	denotes	dilated
T17151	36596-36600	NNS	denotes	tips
T17152	36601-36604	CC	denotes	and
T17153	36605-36612	VBN	denotes	reduced
T17154	36613-36620	NNS	denotes	numbers
T17155	36621-36623	IN	denotes	of
T17156	36624-36628	NNS	denotes	tips
T17157	36628-36629	.	denotes	.
T17158	36629-36749	sentence	denotes	In addition, in Pygo2 mutants there was an expansion of the zone of metanephric mesenchyme that caps the ureteric buds.
T17159	36630-36632	IN	denotes	In
T17161	36633-36641	NN	denotes	addition
T17162	36641-36643	,	denotes	,
T17163	36643-36645	IN	denotes	in
T17164	36646-36651	NN	denotes	Pygo2
T17165	36652-36659	NNS	denotes	mutants
T17166	36660-36665	EX	denotes	there
T17160	36666-36669	VBD	denotes	was
T17167	36670-36672	DT	denotes	an
T17168	36673-36682	NN	denotes	expansion
T17169	36683-36685	IN	denotes	of
T17170	36686-36689	DT	denotes	the
T17171	36690-36694	NN	denotes	zone
T17172	36695-36697	IN	denotes	of
T17173	36698-36709	JJ	denotes	metanephric
T17174	36710-36720	NN	denotes	mesenchyme
T17175	36721-36725	WDT	denotes	that
T17176	36726-36730	VBZ	denotes	caps
T17177	36731-36734	DT	denotes	the
T17179	36735-36743	JJ	denotes	ureteric
T17178	36744-36748	NNS	denotes	buds
T17180	36748-36749	.	denotes	.
T17181	36749-36859	sentence	denotes	Nevertheless, nephron formation proceeded remarkably normally, even in Pygo1/Pygo2 double-homozygous mutants.
T17182	36750-36762	RB	denotes	Nevertheless
T17184	36762-36764	,	denotes	,
T17185	36764-36771	NN	denotes	nephron
T17186	36772-36781	NN	denotes	formation
T17183	36782-36791	VBD	denotes	proceeded
T17187	36792-36802	RB	denotes	remarkably
T17188	36803-36811	RB	denotes	normally
T17189	36811-36813	,	denotes	,
T17190	36813-36817	RB	denotes	even
T17191	36818-36820	IN	denotes	in
T17192	36821-36826	NN	denotes	Pygo1
T17194	36826-36827	HYPH	denotes	/
T17193	36827-36832	NN	denotes	Pygo2
T17196	36833-36839	JJ	denotes	double
T17197	36839-36840	HYPH	denotes	-
T17195	36840-36850	JJ	denotes	homozygous
T17198	36851-36858	NNS	denotes	mutants
T17199	36858-36859	.	denotes	.
T17200	36859-37032	sentence	denotes	This was surprising considering the importance of the Pygopus gene in canonical Wnt signaling in Drosophila, and the importance of canonical Wnt signaling in nephrogenesis.
T17201	36860-36864	DT	denotes	This
T17202	36865-36868	VBD	denotes	was
T17203	36869-36879	JJ	denotes	surprising
T17204	36880-36891	VBG	denotes	considering
T17205	36892-36895	DT	denotes	the
T17206	36896-36906	NN	denotes	importance
T17207	36907-36909	IN	denotes	of
T17208	36910-36913	DT	denotes	the
T17210	36914-36921	NN	denotes	Pygopus
T17209	36922-36926	NN	denotes	gene
T17211	36927-36929	IN	denotes	in
T17212	36930-36939	JJ	denotes	canonical
T17214	36940-36943	NN	denotes	Wnt
T17213	36944-36953	NN	denotes	signaling
T17215	36954-36956	IN	denotes	in
T17216	36957-36967	NNP	denotes	Drosophila
T17217	36967-36969	,	denotes	,
T17218	36969-36972	CC	denotes	and
T17219	36973-36976	DT	denotes	the
T17220	36977-36987	NN	denotes	importance
T17221	36988-36990	IN	denotes	of
T17222	36991-37000	JJ	denotes	canonical
T17224	37001-37004	NN	denotes	Wnt
T17223	37005-37014	NN	denotes	signaling
T17225	37015-37017	IN	denotes	in
T17226	37018-37031	NN	denotes	nephrogenesis
T17227	37031-37032	.	denotes	.
T17228	37032-37164	sentence	denotes	The results argue that the mammalian Pygopus genes are, in most developing systems, only quantitative transducers of Wnt signaling.
T17229	37033-37036	DT	denotes	The
T17230	37037-37044	NNS	denotes	results
T17231	37045-37050	VBP	denotes	argue
T17232	37051-37055	IN	denotes	that
T17234	37056-37059	DT	denotes	the
T17236	37060-37069	JJ	denotes	mammalian
T17237	37070-37077	NN	denotes	Pygopus
T17235	37078-37083	NNS	denotes	genes
T17233	37084-37087	VBP	denotes	are
T17238	37087-37089	,	denotes	,
T17239	37089-37091	IN	denotes	in
T17240	37092-37096	JJS	denotes	most
T17242	37097-37107	VBG	denotes	developing
T17241	37108-37115	NNS	denotes	systems
T17243	37115-37117	,	denotes	,
T17244	37117-37121	RB	denotes	only
T17246	37122-37134	JJ	denotes	quantitative
T17245	37135-37146	NNS	denotes	transducers
T17247	37147-37149	IN	denotes	of
T17248	37150-37153	NN	denotes	Wnt
T17249	37154-37163	NN	denotes	signaling
T17250	37163-37164	.	denotes	.
T17251	37164-37391	sentence	denotes	Previous cell culture studies [32,33] and the reduced BAT-gal transgene reporter expression in the Pygo1/Pygo2 knockout mice described in this report, do confirm an evolutionarily conserved function in canonical Wnt signaling.
T17252	37165-37173	JJ	denotes	Previous
T17254	37174-37178	NN	denotes	cell
T17255	37179-37186	NN	denotes	culture
T17253	37187-37194	NNS	denotes	studies
T17257	37195-37196	-LRB-	denotes	[
T17259	37196-37198	CD	denotes	32
T17260	37198-37199	,	denotes	,
T17258	37199-37201	CD	denotes	33
T17261	37201-37202	-RRB-	denotes	]
T17262	37203-37206	CC	denotes	and
T17263	37207-37210	DT	denotes	the
T17265	37211-37218	VBN	denotes	reduced
T17266	37219-37222	NN	denotes	BAT
T17268	37222-37223	HYPH	denotes	-
T17267	37223-37226	NN	denotes	gal
T17269	37227-37236	NN	denotes	transgene
T17270	37237-37245	NN	denotes	reporter
T17264	37246-37256	NN	denotes	expression
T17271	37257-37259	IN	denotes	in
T17272	37260-37263	DT	denotes	the
T17274	37264-37269	NN	denotes	Pygo1
T17276	37269-37270	HYPH	denotes	/
T17275	37270-37275	NN	denotes	Pygo2
T17277	37276-37284	NN	denotes	knockout
T17273	37285-37289	NNS	denotes	mice
T17278	37290-37299	VBN	denotes	described
T17279	37300-37302	IN	denotes	in
T17280	37303-37307	DT	denotes	this
T17281	37308-37314	NN	denotes	report
T17282	37314-37316	,	denotes	,
T17283	37316-37318	VBP	denotes	do
T17256	37319-37326	VB	denotes	confirm
T17284	37327-37329	DT	denotes	an
T17286	37330-37344	RB	denotes	evolutionarily
T17287	37345-37354	VBN	denotes	conserved
T17285	37355-37363	NN	denotes	function
T17288	37364-37366	IN	denotes	in
T17289	37367-37376	JJ	denotes	canonical
T17291	37377-37380	NN	denotes	Wnt
T17290	37381-37390	NN	denotes	signaling
T17292	37390-37391	.	denotes	.
T17293	37391-37491	sentence	denotes	In mammals, however, the phenotypic effects of Pygopus mutation are much milder than in Drosophila.
T17294	37392-37394	IN	denotes	In
T17296	37395-37402	NNS	denotes	mammals
T17297	37402-37404	,	denotes	,
T17298	37404-37411	RB	denotes	however
T17299	37411-37413	,	denotes	,
T17300	37413-37416	DT	denotes	the
T17302	37417-37427	JJ	denotes	phenotypic
T17301	37428-37435	NNS	denotes	effects
T17303	37436-37438	IN	denotes	of
T17304	37439-37446	NN	denotes	Pygopus
T17305	37447-37455	NN	denotes	mutation
T17295	37456-37459	VBP	denotes	are
T17306	37460-37464	RB	denotes	much
T17307	37465-37471	JJR	denotes	milder
T17308	37472-37476	IN	denotes	than
T17309	37477-37479	IN	denotes	in
T17310	37480-37490	NNP	denotes	Drosophila
T17311	37490-37491	.	denotes	.
T17312	37491-37652	sentence	denotes	The degree of importance of the Pyg1/2 genes in Wnt signaling was context-dependent, but in general, mammalian organogenesis remained intact in Pygopus mutants.
T17313	37492-37495	DT	denotes	The
T17314	37496-37502	NN	denotes	degree
T17316	37503-37505	IN	denotes	of
T17317	37506-37516	NN	denotes	importance
T17318	37517-37519	IN	denotes	of
T17319	37520-37523	DT	denotes	the
T17321	37524-37528	NN	denotes	Pyg1
T17322	37528-37529	HYPH	denotes	/
T17323	37529-37530	CD	denotes	2
T17320	37531-37536	NNS	denotes	genes
T17324	37537-37539	IN	denotes	in
T17325	37540-37543	NN	denotes	Wnt
T17326	37544-37553	NN	denotes	signaling
T17315	37554-37557	VBD	denotes	was
T17327	37558-37565	NN	denotes	context
T17329	37565-37566	HYPH	denotes	-
T17328	37566-37575	JJ	denotes	dependent
T17330	37575-37577	,	denotes	,
T17331	37577-37580	CC	denotes	but
T17332	37581-37583	IN	denotes	in
T17334	37584-37591	JJ	denotes	general
T17335	37591-37593	,	denotes	,
T17336	37593-37602	JJ	denotes	mammalian
T17337	37603-37616	NN	denotes	organogenesis
T17333	37617-37625	VBD	denotes	remained
T17338	37626-37632	JJ	denotes	intact
T17339	37633-37635	IN	denotes	in
T17340	37636-37643	NNP	denotes	Pygopus
T17341	37644-37651	NNS	denotes	mutants
T17342	37651-37652	.	denotes	.
T17343	37652-37780	sentence	denotes	Perhaps the simplest explanation is that in mammals, other genes show partial functional redundancy with the two Pygopus genes.
T17344	37653-37660	RB	denotes	Perhaps
T17346	37661-37664	DT	denotes	the
T17348	37665-37673	JJS	denotes	simplest
T17347	37674-37685	NN	denotes	explanation
T17345	37686-37688	VBZ	denotes	is
T17349	37689-37693	IN	denotes	that
T17351	37694-37696	IN	denotes	in
T17352	37697-37704	NNS	denotes	mammals
T17353	37704-37706	,	denotes	,
T17354	37706-37711	JJ	denotes	other
T17355	37712-37717	NNS	denotes	genes
T17350	37718-37722	VBP	denotes	show
T17356	37723-37730	JJ	denotes	partial
T17358	37731-37741	JJ	denotes	functional
T17357	37742-37752	NN	denotes	redundancy
T17359	37753-37757	IN	denotes	with
T17360	37758-37761	DT	denotes	the
T17362	37762-37765	CD	denotes	two
T17363	37766-37773	NN	denotes	Pygopus
T17361	37774-37779	NNS	denotes	genes
T17364	37779-37780	.	denotes	.
T17365	37780-37991	sentence	denotes	The β-catenin transcription-factor complex includes a large and growing number of proteins , some of which may share the nuclear localization and/or transcription activation functions of Pygo1/Pygo2 in mammals.
T17366	37781-37784	DT	denotes	The
T17368	37785-37786	NN	denotes	β
T17370	37786-37787	HYPH	denotes	-
T17369	37787-37794	NN	denotes	catenin
T17371	37795-37808	NN	denotes	transcription
T17373	37808-37809	HYPH	denotes	-
T17372	37809-37815	NN	denotes	factor
T17367	37816-37823	NN	denotes	complex
T17374	37824-37832	VBZ	denotes	includes
T17375	37833-37834	DT	denotes	a
T17377	37835-37840	JJ	denotes	large
T17378	37841-37844	CC	denotes	and
T17379	37845-37852	VBG	denotes	growing
T17376	37853-37859	NN	denotes	number
T17380	37860-37862	IN	denotes	of
T17381	37863-37871	NN	denotes	proteins
T17382	37872-37874	,	denotes	,
T17383	37874-37878	DT	denotes	some
T17385	37879-37881	IN	denotes	of
T17386	37882-37887	WDT	denotes	which
T17387	37888-37891	MD	denotes	may
T17384	37892-37897	VB	denotes	share
T17388	37898-37901	DT	denotes	the
T17390	37902-37909	JJ	denotes	nuclear
T17391	37910-37922	NN	denotes	localization
T17392	37923-37926	CC	denotes	and
T17393	37926-37927	HYPH	denotes	/
T17394	37927-37929	CC	denotes	or
T17395	37930-37943	NN	denotes	transcription
T17396	37944-37954	NN	denotes	activation
T17389	37955-37964	NNS	denotes	functions
T17397	37965-37967	IN	denotes	of
T17398	37968-37973	NN	denotes	Pygo1
T17400	37973-37974	HYPH	denotes	/
T17399	37974-37979	NN	denotes	Pygo2
T17401	37980-37982	IN	denotes	in
T17402	37983-37990	NNS	denotes	mammals
T17403	37990-37991	.	denotes	.
T17404	37991-38074	sentence	denotes	The identities and roles of these Pygopus redundant genes remain to be determined.
T17405	37992-37995	DT	denotes	The
T17406	37996-38006	NNS	denotes	identities
T17408	38007-38010	CC	denotes	and
T17409	38011-38016	NNS	denotes	roles
T17410	38017-38019	IN	denotes	of
T17411	38020-38025	DT	denotes	these
T17413	38026-38033	NN	denotes	Pygopus
T17414	38034-38043	JJ	denotes	redundant
T17412	38044-38049	NNS	denotes	genes
T17407	38050-38056	VBP	denotes	remain
T17415	38057-38059	TO	denotes	to
T17417	38060-38062	VB	denotes	be
T17416	38063-38073	VBN	denotes	determined
T17418	38073-38074	.	denotes	.
T18189	38085-38094	NN	denotes	Targeting
T18190	38095-38097	IN	denotes	of
T18191	38098-38103	NN	denotes	Pygo1
T18192	38104-38107	CC	denotes	and
T18193	38108-38113	NN	denotes	Pygo2
T18194	38113-38258	sentence	denotes	Pygo1 and Pygo2 genes were each targeted by flanking the critical coding regions of the third exon containing the PHD motif with LoxP sequences.
T18195	38114-38119	NN	denotes	Pygo1
T18197	38120-38123	CC	denotes	and
T18198	38124-38129	NN	denotes	Pygo2
T18196	38130-38135	NNS	denotes	genes
T18200	38136-38140	VBD	denotes	were
T18201	38141-38145	RB	denotes	each
T18199	38146-38154	VBN	denotes	targeted
T18202	38155-38157	IN	denotes	by
T18203	38158-38166	VBG	denotes	flanking
T18204	38167-38170	DT	denotes	the
T18206	38171-38179	JJ	denotes	critical
T18207	38180-38186	NN	denotes	coding
T18205	38187-38194	NNS	denotes	regions
T18208	38195-38197	IN	denotes	of
T18209	38198-38201	DT	denotes	the
T18211	38202-38207	JJ	denotes	third
T18210	38208-38212	NN	denotes	exon
T18212	38213-38223	VBG	denotes	containing
T18213	38224-38227	DT	denotes	the
T18215	38228-38231	NN	denotes	PHD
T18214	38232-38237	NN	denotes	motif
T18216	38238-38242	IN	denotes	with
T18217	38243-38247	NN	denotes	LoxP
T18218	38248-38257	NNS	denotes	sequences
T18219	38257-38258	.	denotes	.
T18220	38258-38569	sentence	denotes	Targeting constructs were made using the Flp/Cre vector previously described [34], which carries a neomycin-resistance gene flanked by FRT sequences, and three unique restriction sites for subcloning the two blocks of homology for driving recombination, and the critical region to be flanked by LoxP sequences.
T18221	38259-38268	NN	denotes	Targeting
T18222	38269-38279	NNS	denotes	constructs
T18224	38280-38284	VBD	denotes	were
T18223	38285-38289	VBN	denotes	made
T18225	38290-38295	VBG	denotes	using
T18226	38296-38299	DT	denotes	the
T18228	38300-38303	NN	denotes	Flp
T18230	38303-38304	HYPH	denotes	/
T18229	38304-38307	NN	denotes	Cre
T18227	38308-38314	NN	denotes	vector
T18231	38315-38325	RB	denotes	previously
T18232	38326-38335	VBN	denotes	described
T18233	38336-38337	-LRB-	denotes	[
T18234	38337-38339	CD	denotes	34
T18235	38339-38340	-RRB-	denotes	]
T18236	38340-38342	,	denotes	,
T18237	38342-38347	WDT	denotes	which
T18238	38348-38355	VBZ	denotes	carries
T18239	38356-38357	DT	denotes	a
T18241	38358-38366	NN	denotes	neomycin
T18243	38366-38367	HYPH	denotes	-
T18242	38367-38377	NN	denotes	resistance
T18240	38378-38382	NN	denotes	gene
T18244	38383-38390	VBN	denotes	flanked
T18245	38391-38393	IN	denotes	by
T18246	38394-38397	NN	denotes	FRT
T18247	38398-38407	NNS	denotes	sequences
T18248	38407-38409	,	denotes	,
T18249	38409-38412	CC	denotes	and
T18250	38413-38418	CD	denotes	three
T18252	38419-38425	JJ	denotes	unique
T18253	38426-38437	NN	denotes	restriction
T18251	38438-38443	NNS	denotes	sites
T18254	38444-38447	IN	denotes	for
T18255	38448-38458	VBG	denotes	subcloning
T18256	38459-38462	DT	denotes	the
T18258	38463-38466	CD	denotes	two
T18257	38467-38473	NNS	denotes	blocks
T18259	38474-38476	IN	denotes	of
T18260	38477-38485	NN	denotes	homology
T18261	38486-38489	IN	denotes	for
T18262	38490-38497	VBG	denotes	driving
T18263	38498-38511	NN	denotes	recombination
T18264	38511-38513	,	denotes	,
T18265	38513-38516	CC	denotes	and
T18266	38517-38520	DT	denotes	the
T18268	38521-38529	JJ	denotes	critical
T18267	38530-38536	NN	denotes	region
T18269	38537-38539	TO	denotes	to
T18271	38540-38542	VB	denotes	be
T18270	38543-38550	VBN	denotes	flanked
T18272	38551-38553	IN	denotes	by
T18273	38554-38558	NN	denotes	LoxP
T18274	38559-38568	NNS	denotes	sequences
T18275	38568-38569	.	denotes	.
T18276	38569-38678	sentence	denotes	For each construct, the three genomic segments required for subcloning were made by PCR from RI ES cell DNA.
T18277	38570-38573	IN	denotes	For
T18279	38574-38578	DT	denotes	each
T18280	38579-38588	NN	denotes	construct
T18281	38588-38590	,	denotes	,
T18282	38590-38593	DT	denotes	the
T18284	38594-38599	CD	denotes	three
T18285	38600-38607	JJ	denotes	genomic
T18283	38608-38616	NNS	denotes	segments
T18286	38617-38625	VBN	denotes	required
T18287	38626-38629	IN	denotes	for
T18288	38630-38640	NN	denotes	subcloning
T18289	38641-38645	VBD	denotes	were
T18278	38646-38650	VBN	denotes	made
T18290	38651-38653	IN	denotes	by
T18291	38654-38657	NN	denotes	PCR
T18292	38658-38662	IN	denotes	from
T18293	38663-38665	NN	denotes	RI
T18295	38666-38668	NN	denotes	ES
T18296	38669-38673	NN	denotes	cell
T18294	38674-38677	NN	denotes	DNA
T18297	38677-38678	.	denotes	.
T18298	38678-38743	sentence	denotes	The resulting targeting constructs were confirmed by sequencing.
T18299	38679-38682	DT	denotes	The
T18301	38683-38692	VBG	denotes	resulting
T18302	38693-38702	NN	denotes	targeting
T18300	38703-38713	NNS	denotes	constructs
T18304	38714-38718	VBD	denotes	were
T18303	38719-38728	VBN	denotes	confirmed
T18305	38729-38731	IN	denotes	by
T18306	38732-38742	NN	denotes	sequencing
T18307	38742-38743	.	denotes	.
T18308	38743-39003	sentence	denotes	For Pygo1, the genomic sequences used for PCR were: 5' forward, GTGAAGGAGAGATGGATAAGTATG; 5' back, TAGACCCTAACCACCTACAAG; exon forward, GGTTAGGGTCTATGTGCTGG; exon back, TCACCAAATCTCTGTTCTACAC; 3' forward, TGTGTAGAACAGAGATTTGGTG; 3' back, CAGTGAAGAAAGAGGGTCAG.
T18309	38744-38747	IN	denotes	For
T18311	38748-38753	NN	denotes	Pygo1
T18312	38753-38755	,	denotes	,
T18313	38755-38758	DT	denotes	the
T18315	38759-38766	JJ	denotes	genomic
T18314	38767-38776	NNS	denotes	sequences
T18316	38777-38781	VBN	denotes	used
T18317	38782-38785	IN	denotes	for
T18318	38786-38789	NN	denotes	PCR
T18310	38790-38794	VBD	denotes	were
T18319	38794-38796	:	denotes	:
T18320	38796-38797	CD	denotes	5
T18322	38797-38798	SYM	denotes	'
T18321	38799-38806	JJ	denotes	forward
T18324	38806-38808	,	denotes	,
T18323	38808-38832	NN	denotes	GTGAAGGAGAGATGGATAAGTATG
T18325	38832-38833	:	denotes	;
T18326	38834-38835	CD	denotes	5
T18328	38835-38836	SYM	denotes	'
T18327	38837-38841	JJ	denotes	back
T18330	38841-38843	,	denotes	,
T18329	38843-38864	NN	denotes	TAGACCCTAACCACCTACAAG
T18331	38864-38865	:	denotes	;
T18332	38866-38870	NN	denotes	exon
T18333	38871-38878	JJ	denotes	forward
T18335	38878-38880	,	denotes	,
T18334	38880-38900	NN	denotes	GGTTAGGGTCTATGTGCTGG
T18336	38900-38901	:	denotes	;
T18337	38902-38906	NN	denotes	exon
T18338	38907-38911	JJ	denotes	back
T18340	38911-38913	,	denotes	,
T18339	38913-38935	NN	denotes	TCACCAAATCTCTGTTCTACAC
T18341	38935-38936	:	denotes	;
T18342	38937-38938	CD	denotes	3
T18344	38938-38939	SYM	denotes	'
T18343	38940-38947	JJ	denotes	forward
T18346	38947-38949	,	denotes	,
T18345	38949-38971	NN	denotes	TGTGTAGAACAGAGATTTGGTG
T18347	38971-38972	:	denotes	;
T18348	38973-38974	CD	denotes	3
T18350	38974-38975	SYM	denotes	'
T18349	38976-38980	JJ	denotes	back
T18352	38980-38982	,	denotes	,
T18351	38982-39002	NN	denotes	CAGTGAAGAAAGAGGGTCAG
T18353	39002-39003	.	denotes	.
T18354	39003-39226	sentence	denotes	For Pygo2, the genomic sequences used for PCR were: GCCTGGGTTGCTTGTCTTCTG and CCACCTTACTTGTGTGTGAGGATACATAC, CCAAGTCCCAGCATCTCTTAC and CCAGTCATACCAGCAACAAG, and exon sequences TGGGTGCTGGGAACAGAAC and CAACAACAACAGAAGACAAGC.
T18355	39004-39007	IN	denotes	For
T18357	39008-39013	NN	denotes	Pygo2
T18358	39013-39015	,	denotes	,
T18359	39015-39018	DT	denotes	the
T18361	39019-39026	JJ	denotes	genomic
T18360	39027-39036	NNS	denotes	sequences
T18362	39037-39041	VBN	denotes	used
T18363	39042-39045	IN	denotes	for
T18364	39046-39049	NN	denotes	PCR
T18356	39050-39054	VBD	denotes	were
T18365	39054-39056	:	denotes	:
T18366	39056-39077	NN	denotes	GCCTGGGTTGCTTGTCTTCTG
T18367	39078-39081	CC	denotes	and
T18368	39082-39111	NN	denotes	CCACCTTACTTGTGTGTGAGGATACATAC
T18369	39111-39113	,	denotes	,
T18370	39113-39134	NN	denotes	CCAAGTCCCAGCATCTCTTAC
T18371	39135-39138	CC	denotes	and
T18372	39139-39159	NN	denotes	CCAGTCATACCAGCAACAAG
T18373	39159-39161	,	denotes	,
T18374	39161-39164	CC	denotes	and
T18375	39165-39169	NN	denotes	exon
T18376	39170-39179	NNS	denotes	sequences
T18377	39180-39199	NN	denotes	TGGGTGCTGGGAACAGAAC
T18378	39200-39203	CC	denotes	and
T18379	39204-39225	NN	denotes	CAACAACAACAGAAGACAAGC
T18380	39225-39226	.	denotes	.
T18381	39226-39390	sentence	denotes	Linearized constructs were electroporated into RI ES cells, and resulting targeted ES cells used for C57/Bl6 blastocyst injections according to standard protocols.
T18382	39227-39237	VBN	denotes	Linearized
T18383	39238-39248	NNS	denotes	constructs
T18385	39249-39253	VBD	denotes	were
T18384	39254-39268	VBN	denotes	electroporated
T18386	39269-39273	IN	denotes	into
T18387	39274-39276	NN	denotes	RI
T18389	39277-39279	NN	denotes	ES
T18388	39280-39285	NNS	denotes	cells
T18390	39285-39287	,	denotes	,
T18391	39287-39290	CC	denotes	and
T18392	39291-39300	VBG	denotes	resulting
T18394	39301-39309	VBN	denotes	targeted
T18395	39310-39312	NN	denotes	ES
T18393	39313-39318	NNS	denotes	cells
T18396	39319-39323	VBN	denotes	used
T18397	39324-39327	IN	denotes	for
T18398	39328-39331	NN	denotes	C57
T18400	39331-39332	HYPH	denotes	/
T18399	39332-39335	NN	denotes	Bl6
T18402	39336-39346	NN	denotes	blastocyst
T18401	39347-39357	NNS	denotes	injections
T18403	39358-39367	VBG	denotes	according
T18404	39368-39370	IN	denotes	to
T18405	39371-39379	JJ	denotes	standard
T18406	39380-39389	NNS	denotes	protocols
T18407	39389-39390	.	denotes	.
T18408	39390-39517	sentence	denotes	Resulting chimeras were mated with Swiss Black mice, and the targeted stocks maintained on a mixed 129/Swiss Black background.
T18409	39391-39400	VBG	denotes	Resulting
T18410	39401-39409	NNS	denotes	chimeras
T18412	39410-39414	VBD	denotes	were
T18411	39415-39420	VBN	denotes	mated
T18413	39421-39425	IN	denotes	with
T18414	39426-39431	NNP	denotes	Swiss
T18415	39432-39437	NNP	denotes	Black
T18416	39438-39442	NNS	denotes	mice
T18417	39442-39444	,	denotes	,
T18418	39444-39447	CC	denotes	and
T18419	39448-39451	DT	denotes	the
T18421	39452-39460	VBN	denotes	targeted
T18420	39461-39467	NNS	denotes	stocks
T18422	39468-39478	VBN	denotes	maintained
T18423	39479-39481	IN	denotes	on
T18424	39482-39483	DT	denotes	a
T18426	39484-39489	VBN	denotes	mixed
T18427	39490-39493	CD	denotes	129
T18429	39493-39494	HYPH	denotes	/
T18430	39494-39499	NNP	denotes	Swiss
T18428	39500-39505	NNP	denotes	Black
T18425	39506-39516	NN	denotes	background
T18431	39516-39517	.	denotes	.
T18432	39517-39641	sentence	denotes	Germline null alleles of both Pygo1 and Pygo2 were generated by mating heterozygous floxed mice with the CMV-Cre mice [15].
T18433	39518-39526	NN	denotes	Germline
T18435	39527-39531	JJ	denotes	null
T18434	39532-39539	NNS	denotes	alleles
T18437	39540-39542	IN	denotes	of
T18438	39543-39547	CC	denotes	both
T18439	39548-39553	NN	denotes	Pygo1
T18440	39554-39557	CC	denotes	and
T18441	39558-39563	NN	denotes	Pygo2
T18442	39564-39568	VBD	denotes	were
T18436	39569-39578	VBN	denotes	generated
T18443	39579-39581	IN	denotes	by
T18444	39582-39588	VBG	denotes	mating
T18445	39589-39601	JJ	denotes	heterozygous
T18447	39602-39608	VBN	denotes	floxed
T18446	39609-39613	NNS	denotes	mice
T18448	39614-39618	IN	denotes	with
T18449	39619-39622	DT	denotes	the
T18451	39623-39626	NN	denotes	CMV
T18453	39626-39627	HYPH	denotes	-
T18452	39627-39630	NN	denotes	Cre
T18450	39631-39635	NNS	denotes	mice
T18454	39636-39637	-LRB-	denotes	[
T18455	39637-39639	CD	denotes	15
T18456	39639-39640	-RRB-	denotes	]
T18457	39640-39641	.	denotes	.
T18458	39641-40033	sentence	denotes	The sequences of the primers used for genotyping PCR were: Pygo2 null allele, forward (F) CCTGGATTCTTGTTGCTGGTATG; reverse (R) AAGGTATTTGGTGCTCCGAGGG; Pygo2 WT or floxed allele, F TGTCTTGATGACAGCGTTTAGCC, R AGATTCAGTAAGCTGAGCCTGGTG; Pygo1 null allele, F AGTTTGAAATAGCGACGAGTTTGAG, R 5'-CACTTCTGCCCCTCTCTTTGC; Pygo1 WT or floxed allele, F AAGCGTGCCTCATCTCCATCCCTAAG, R GCCCTCCCCGACGTTTATATTG.
T18459	39642-39645	DT	denotes	The
T18460	39646-39655	NNS	denotes	sequences
T18462	39656-39658	IN	denotes	of
T18463	39659-39662	DT	denotes	the
T18464	39663-39670	NNS	denotes	primers
T18465	39671-39675	VBN	denotes	used
T18466	39676-39679	IN	denotes	for
T18467	39680-39690	VBG	denotes	genotyping
T18468	39691-39694	NN	denotes	PCR
T18461	39695-39699	VBD	denotes	were
T18469	39699-39701	:	denotes	:
T18470	39701-39706	NN	denotes	Pygo2
T18471	39707-39711	JJ	denotes	null
T18472	39712-39718	NN	denotes	allele
T18473	39718-39720	,	denotes	,
T18474	39720-39727	RB	denotes	forward
T18476	39728-39729	-LRB-	denotes	(
T18477	39729-39730	NN	denotes	F
T18478	39730-39731	-RRB-	denotes	)
T18475	39732-39755	NN	denotes	CCTGGATTCTTGTTGCTGGTATG
T18479	39755-39756	:	denotes	;
T18480	39757-39764	RB	denotes	reverse
T18482	39765-39766	-LRB-	denotes	(
T18483	39766-39767	NN	denotes	R
T18484	39767-39768	-RRB-	denotes	)
T18481	39769-39791	NN	denotes	AAGGTATTTGGTGCTCCGAGGG
T18485	39791-39792	:	denotes	;
T18486	39793-39798	NN	denotes	Pygo2
T18488	39799-39801	NN	denotes	WT
T18489	39802-39804	CC	denotes	or
T18490	39805-39811	VBN	denotes	floxed
T18487	39812-39818	NN	denotes	allele
T18491	39818-39820	,	denotes	,
T18492	39820-39821	NN	denotes	F
T18493	39822-39845	NN	denotes	TGTCTTGATGACAGCGTTTAGCC
T18494	39845-39847	,	denotes	,
T18495	39847-39848	NN	denotes	R
T18496	39849-39873	NN	denotes	AGATTCAGTAAGCTGAGCCTGGTG
T18497	39873-39874	:	denotes	;
T18498	39875-39880	NN	denotes	Pygo1
T18499	39881-39885	JJ	denotes	null
T18500	39886-39892	NN	denotes	allele
T18501	39892-39894	,	denotes	,
T18502	39894-39895	NN	denotes	F
T18503	39896-39921	NN	denotes	AGTTTGAAATAGCGACGAGTTTGAG
T18504	39921-39923	,	denotes	,
T18505	39923-39924	NN	denotes	R
T18506	39925-39926	CD	denotes	5
T18508	39926-39927	SYM	denotes	'
T18509	39927-39928	HYPH	denotes	-
T18507	39928-39949	NN	denotes	CACTTCTGCCCCTCTCTTTGC
T18510	39949-39950	:	denotes	;
T18511	39951-39956	NN	denotes	Pygo1
T18513	39957-39959	NN	denotes	WT
T18514	39960-39962	CC	denotes	or
T18515	39963-39969	VBN	denotes	floxed
T18512	39970-39976	NN	denotes	allele
T18516	39976-39978	,	denotes	,
T18517	39978-39979	NN	denotes	F
T18518	39980-40006	NN	denotes	AAGCGTGCCTCATCTCCATCCCTAAG
T18519	40006-40008	,	denotes	,
T18520	40008-40009	NN	denotes	R
T18521	40010-40032	NN	denotes	GCCCTCCCCGACGTTTATATTG
T18522	40032-40033	.	denotes	.
T18523	40033-40107	sentence	denotes	The noon of the day that vaginal plugs were observed was designated E0.5.
T18524	40034-40037	DT	denotes	The
T18525	40038-40042	NN	denotes	noon
T18527	40043-40045	IN	denotes	of
T18528	40046-40049	DT	denotes	the
T18529	40050-40053	NN	denotes	day
T18530	40054-40058	IN	denotes	that
T18532	40059-40066	JJ	denotes	vaginal
T18533	40067-40072	NNS	denotes	plugs
T18534	40073-40077	VBD	denotes	were
T18531	40078-40086	VBN	denotes	observed
T18535	40087-40090	VBD	denotes	was
T18526	40091-40101	VBN	denotes	designated
T18536	40102-40106	NN	denotes	E0.5
T18537	40106-40107	.	denotes	.
T19104	40109-40117	JJ	denotes	Confocal
T19105	40118-40128	NN	denotes	microscopy
T19106	40128-40298	sentence	denotes	Kidneys were dissected, fixed in paraformaldehyde, treated with methanol, and washed with PBS containing Tween-20 (PBS-T) prior to treatment with lectins and antibodies.
T19107	40129-40136	NNS	denotes	Kidneys
T19109	40137-40141	VBD	denotes	were
T19108	40142-40151	VBN	denotes	dissected
T19110	40151-40153	,	denotes	,
T19111	40153-40158	VBN	denotes	fixed
T19112	40159-40161	IN	denotes	in
T19113	40162-40178	NN	denotes	paraformaldehyde
T19114	40178-40180	,	denotes	,
T19115	40180-40187	VBN	denotes	treated
T19116	40188-40192	IN	denotes	with
T19117	40193-40201	NN	denotes	methanol
T19118	40201-40203	,	denotes	,
T19119	40203-40206	CC	denotes	and
T19120	40207-40213	VBN	denotes	washed
T19121	40214-40218	IN	denotes	with
T19122	40219-40222	NN	denotes	PBS
T19123	40223-40233	VBG	denotes	containing
T19124	40234-40239	NN	denotes	Tween
T19125	40239-40240	HYPH	denotes	-
T19126	40240-40242	CD	denotes	20
T19127	40243-40244	-LRB-	denotes	(
T19128	40244-40247	NN	denotes	PBS
T19130	40247-40248	HYPH	denotes	-
T19129	40248-40249	NN	denotes	T
T19131	40249-40250	-RRB-	denotes	)
T19132	40251-40256	JJ	denotes	prior
T19133	40257-40259	IN	denotes	to
T19134	40260-40269	NN	denotes	treatment
T19135	40270-40274	IN	denotes	with
T19136	40275-40282	NNS	denotes	lectins
T19137	40283-40286	CC	denotes	and
T19138	40287-40297	NNS	denotes	antibodies
T19139	40297-40298	.	denotes	.
T19140	40298-40507	sentence	denotes	PBS-T was used for incubations of the tissues with fluoroscein-conjugated Dilichos biflorus aggutinin (DBA, Vector; Burlingame, USA), and PBS-T plus 2% goat serum was used for incubations with the antibodies.
T19141	40299-40302	NN	denotes	PBS
T19143	40302-40303	HYPH	denotes	-
T19142	40303-40304	NN	denotes	T
T19145	40305-40308	VBD	denotes	was
T19144	40309-40313	VBN	denotes	used
T19146	40314-40317	IN	denotes	for
T19147	40318-40329	NNS	denotes	incubations
T19148	40330-40332	IN	denotes	of
T19149	40333-40336	DT	denotes	the
T19150	40337-40344	NNS	denotes	tissues
T19151	40345-40349	IN	denotes	with
T19152	40350-40361	NN	denotes	fluoroscein
T19154	40361-40362	HYPH	denotes	-
T19153	40362-40372	VBN	denotes	conjugated
T19156	40373-40381	NNP	denotes	Dilichos
T19157	40382-40390	NN	denotes	biflorus
T19155	40391-40400	NN	denotes	aggutinin
T19158	40401-40402	-LRB-	denotes	(
T19159	40402-40405	NNP	denotes	DBA
T19160	40405-40407	,	denotes	,
T19161	40407-40413	NNP	denotes	Vector
T19162	40413-40414	:	denotes	;
T19163	40415-40425	NNP	denotes	Burlingame
T19164	40425-40427	,	denotes	,
T19165	40427-40430	NNP	denotes	USA
T19166	40430-40431	-RRB-	denotes	)
T19167	40431-40433	,	denotes	,
T19168	40433-40436	CC	denotes	and
T19169	40437-40440	NN	denotes	PBS
T19171	40440-40441	HYPH	denotes	-
T19170	40441-40442	NN	denotes	T
T19173	40443-40447	CC	denotes	plus
T19174	40448-40449	CD	denotes	2
T19175	40449-40450	NN	denotes	%
T19177	40451-40455	NN	denotes	goat
T19176	40456-40461	NN	denotes	serum
T19178	40462-40465	VBD	denotes	was
T19172	40466-40470	VBN	denotes	used
T19179	40471-40474	IN	denotes	for
T19180	40475-40486	NNS	denotes	incubations
T19181	40487-40491	IN	denotes	with
T19182	40492-40495	DT	denotes	the
T19183	40496-40506	NNS	denotes	antibodies
T19184	40506-40507	.	denotes	.
T19185	40507-40740	sentence	denotes	The primary antibodies were anti-WT1 (c-19; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-uvomorulin (e-cadherin, Cdh1, Sigma-Aldrich St. Louis, MO, USA), and anti-Cited1 (Neomarkers-Lab Vision Corporation, Fremont, CA, USA).
T19186	40508-40511	DT	denotes	The
T19188	40512-40519	JJ	denotes	primary
T19187	40520-40530	NNS	denotes	antibodies
T19189	40531-40535	VBD	denotes	were
T19190	40536-40544	JJ	denotes	anti-WT1
T19191	40545-40546	-LRB-	denotes	(
T19193	40546-40547	NN	denotes	c
T19194	40547-40548	HYPH	denotes	-
T19195	40548-40550	CD	denotes	19
T19196	40550-40551	:	denotes	;
T19197	40552-40557	NNP	denotes	Santa
T19198	40558-40562	NNP	denotes	Cruz
T19192	40563-40576	NNP	denotes	Biotechnology
T19199	40576-40578	,	denotes	,
T19200	40578-40583	NNP	denotes	Santa
T19201	40584-40588	NNP	denotes	Cruz
T19202	40588-40590	,	denotes	,
T19203	40590-40592	NNP	denotes	CA
T19204	40592-40594	,	denotes	,
T19205	40594-40597	NNP	denotes	USA
T19206	40597-40598	-RRB-	denotes	)
T19207	40598-40600	,	denotes	,
T19208	40600-40615	JJ	denotes	anti-uvomorulin
T19209	40616-40617	-LRB-	denotes	(
T19210	40617-40618	NN	denotes	e
T19212	40618-40619	HYPH	denotes	-
T19211	40619-40627	NN	denotes	cadherin
T19213	40627-40629	,	denotes	,
T19214	40629-40633	NN	denotes	Cdh1
T19215	40633-40635	,	denotes	,
T19217	40635-40640	NNP	denotes	Sigma
T19218	40640-40641	HYPH	denotes	-
T19216	40641-40648	NNP	denotes	Aldrich
T19219	40649-40652	NNP	denotes	St.
T19220	40653-40658	NNP	denotes	Louis
T19221	40658-40660	,	denotes	,
T19222	40660-40662	NNP	denotes	MO
T19223	40662-40664	,	denotes	,
T19224	40664-40667	NNP	denotes	USA
T19225	40667-40668	-RRB-	denotes	)
T19226	40668-40670	,	denotes	,
T19227	40670-40673	CC	denotes	and
T19228	40674-40685	JJ	denotes	anti-Cited1
T19229	40686-40687	-LRB-	denotes	(
T19231	40687-40697	NNP	denotes	Neomarkers
T19232	40697-40698	HYPH	denotes	-
T19233	40698-40701	NNP	denotes	Lab
T19234	40702-40708	NNP	denotes	Vision
T19230	40709-40720	NNP	denotes	Corporation
T19235	40720-40722	,	denotes	,
T19236	40722-40729	NNP	denotes	Fremont
T19237	40729-40731	,	denotes	,
T19238	40731-40733	NNP	denotes	CA
T19239	40733-40735	,	denotes	,
T19240	40735-40738	NNP	denotes	USA
T19241	40738-40739	-RRB-	denotes	)
T19242	40739-40740	.	denotes	.
T19243	40740-40885	sentence	denotes	The secondary antibodies were Alexa 555-conjugated anti-rabbit and Alexa 633-conjugated anti-rat antibodies (Molecular Probes, Eugene, OR, USA).
T19244	40741-40744	DT	denotes	The
T19246	40745-40754	JJ	denotes	secondary
T19245	40755-40765	NNS	denotes	antibodies
T19247	40766-40770	VBD	denotes	were
T19248	40771-40776	NNP	denotes	Alexa
T19250	40777-40780	CD	denotes	555
T19251	40780-40781	HYPH	denotes	-
T19249	40781-40791	VBN	denotes	conjugated
T19252	40792-40803	JJ	denotes	anti-rabbit
T19254	40804-40807	CC	denotes	and
T19255	40808-40813	NNP	denotes	Alexa
T19257	40814-40817	CD	denotes	633
T19258	40817-40818	HYPH	denotes	-
T19256	40818-40828	VBN	denotes	conjugated
T19259	40829-40837	JJ	denotes	anti-rat
T19253	40838-40848	NNS	denotes	antibodies
T19260	40849-40850	-LRB-	denotes	(
T19262	40850-40859	NNP	denotes	Molecular
T19261	40860-40866	NNP	denotes	Probes
T19263	40866-40868	,	denotes	,
T19264	40868-40874	NNP	denotes	Eugene
T19265	40874-40876	,	denotes	,
T19266	40876-40878	NNP	denotes	OR
T19267	40878-40880	,	denotes	,
T19268	40880-40883	NNP	denotes	USA
T19269	40883-40884	-RRB-	denotes	)
T19270	40884-40885	.	denotes	.
T19271	40885-41054	sentence	denotes	The tissues were imaged with a laser scanning microscope (Carl Zeiss, Thornwood, New York, USA) equipped with an argon (488 nm) and two HeNe lasers (543 nm and 633 nm).
T19272	40886-40889	DT	denotes	The
T19273	40890-40897	NNS	denotes	tissues
T19275	40898-40902	VBD	denotes	were
T19274	40903-40909	VBN	denotes	imaged
T19276	40910-40914	IN	denotes	with
T19277	40915-40916	DT	denotes	a
T19279	40917-40922	NN	denotes	laser
T19280	40923-40931	NN	denotes	scanning
T19278	40932-40942	NN	denotes	microscope
T19281	40943-40944	-LRB-	denotes	(
T19283	40944-40948	NNP	denotes	Carl
T19282	40949-40954	NNP	denotes	Zeiss
T19284	40954-40956	,	denotes	,
T19285	40956-40965	NNP	denotes	Thornwood
T19286	40965-40967	,	denotes	,
T19287	40967-40970	NNP	denotes	New
T19288	40971-40975	NNP	denotes	York
T19289	40975-40977	,	denotes	,
T19290	40977-40980	NNP	denotes	USA
T19291	40980-40981	-RRB-	denotes	)
T19292	40982-40990	VBN	denotes	equipped
T19293	40991-40995	IN	denotes	with
T19294	40996-40998	CD	denotes	an
T19295	40999-41004	NN	denotes	argon
T19296	41005-41006	-LRB-	denotes	(
T19298	41006-41009	CD	denotes	488
T19297	41010-41012	NN	denotes	nm
T19299	41012-41013	-RRB-	denotes	)
T19300	41014-41017	CC	denotes	and
T19301	41018-41021	CD	denotes	two
T19302	41022-41026	NN	denotes	HeNe
T19303	41027-41033	NNS	denotes	lasers
T19304	41034-41035	-LRB-	denotes	(
T19306	41035-41038	CD	denotes	543
T19305	41039-41041	NN	denotes	nm
T19307	41042-41045	CC	denotes	and
T19308	41046-41049	CD	denotes	633
T19309	41050-41052	NN	denotes	nm
T19310	41052-41053	-RRB-	denotes	)
T19311	41053-41054	.	denotes	.
T19312	41054-41151	sentence	denotes	Optical sections approximately 2 μm thick were obtained every 5 μm to a depth of at least 65 μm.
T19313	41055-41062	JJ	denotes	Optical
T19314	41063-41071	NNS	denotes	sections
T19316	41072-41085	RB	denotes	approximately
T19317	41086-41087	CD	denotes	2
T19318	41088-41090	NN	denotes	μm
T19319	41091-41096	NN	denotes	thick
T19320	41097-41101	VBD	denotes	were
T19315	41102-41110	VBN	denotes	obtained
T19321	41111-41116	DT	denotes	every
T19323	41117-41118	CD	denotes	5
T19322	41119-41121	NN	denotes	μm
T19324	41122-41124	IN	denotes	to
T19325	41125-41126	DT	denotes	a
T19326	41127-41132	NN	denotes	depth
T19327	41133-41135	IN	denotes	of
T19328	41136-41138	RB	denotes	at
T19330	41139-41144	RBS	denotes	least
T19329	41145-41147	CD	denotes	65
T19331	41148-41150	NN	denotes	μm
T19332	41150-41151	.	denotes	.
T19333	41151-41237	sentence	denotes	The sections began at the surface of the kidney and were on a plane tangential to it.
T19334	41152-41155	DT	denotes	The
T19335	41156-41164	NNS	denotes	sections
T19336	41165-41170	VBD	denotes	began
T19337	41171-41173	IN	denotes	at
T19338	41174-41177	DT	denotes	the
T19339	41178-41185	NN	denotes	surface
T19340	41186-41188	IN	denotes	of
T19341	41189-41192	DT	denotes	the
T19342	41193-41199	NN	denotes	kidney
T19343	41200-41203	CC	denotes	and
T19344	41204-41208	VBD	denotes	were
T19345	41209-41211	IN	denotes	on
T19346	41212-41213	DT	denotes	a
T19347	41214-41219	NN	denotes	plane
T19348	41220-41230	JJ	denotes	tangential
T19349	41231-41233	IN	denotes	to
T19350	41234-41236	PRP	denotes	it
T19351	41236-41237	.	denotes	.
T19352	41237-41320	sentence	denotes	Two Z-stack series were obtained, one from each of the two kidneys of each embryo.
T19353	41238-41241	CD	denotes	Two
T19355	41242-41243	NN	denotes	Z
T19357	41243-41244	HYPH	denotes	-
T19356	41244-41249	NN	denotes	stack
T19354	41250-41256	NNS	denotes	series
T19359	41257-41261	VBD	denotes	were
T19358	41262-41270	VBN	denotes	obtained
T19360	41270-41272	,	denotes	,
T19361	41272-41275	CD	denotes	one
T19362	41276-41280	IN	denotes	from
T19363	41281-41285	DT	denotes	each
T19364	41286-41288	IN	denotes	of
T19365	41289-41292	DT	denotes	the
T19367	41293-41296	CD	denotes	two
T19366	41297-41304	NNS	denotes	kidneys
T19368	41305-41307	IN	denotes	of
T19369	41308-41312	DT	denotes	each
T19370	41313-41319	NN	denotes	embryo
T19371	41319-41320	.	denotes	.
T19372	41320-41426	sentence	denotes	Ureteric bud tips identified by section tracing were counted within a defined area of the confocal image.
T19373	41321-41329	JJ	denotes	Ureteric
T19374	41330-41333	NN	denotes	bud
T19375	41334-41338	NNS	denotes	tips
T19377	41339-41349	VBN	denotes	identified
T19378	41350-41352	IN	denotes	by
T19379	41353-41360	NN	denotes	section
T19380	41361-41368	NN	denotes	tracing
T19381	41369-41373	VBD	denotes	were
T19376	41374-41381	VBN	denotes	counted
T19382	41382-41388	IN	denotes	within
T19383	41389-41390	DT	denotes	a
T19385	41391-41398	VBN	denotes	defined
T19384	41399-41403	NN	denotes	area
T19386	41404-41406	IN	denotes	of
T19387	41407-41410	DT	denotes	the
T19389	41411-41419	JJ	denotes	confocal
T19388	41420-41425	NN	denotes	image
T19390	41425-41426	.	denotes	.
T19486	41428-41430	FW	denotes	In
T19487	41431-41435	FW	denotes	situ
T19488	41436-41449	NN	denotes	hybridization
T19489	41449-41527	sentence	denotes	Whole-mount in situ hybridization was performed as previously described [35].
T19490	41450-41455	JJ	denotes	Whole
T19492	41455-41456	HYPH	denotes	-
T19491	41456-41461	NN	denotes	mount
T19494	41462-41464	FW	denotes	in
T19495	41465-41469	FW	denotes	situ
T19493	41470-41483	NN	denotes	hybridization
T19497	41484-41487	VBD	denotes	was
T19496	41488-41497	VBN	denotes	performed
T19498	41498-41500	IN	denotes	as
T19500	41501-41511	RB	denotes	previously
T19499	41512-41521	VBN	denotes	described
T19501	41522-41523	-LRB-	denotes	[
T19502	41523-41525	CD	denotes	35
T19503	41525-41526	-RRB-	denotes	]
T19504	41526-41527	.	denotes	.
T19505	41527-41589	sentence	denotes	Riboprobes to Wnt11 and Wnt7b were described previously [35].
T19506	41528-41538	NNS	denotes	Riboprobes
T19508	41539-41541	IN	denotes	to
T19509	41542-41547	NN	denotes	Wnt11
T19510	41548-41551	CC	denotes	and
T19511	41552-41557	NN	denotes	Wnt7b
T19512	41558-41562	VBD	denotes	were
T19507	41563-41572	VBN	denotes	described
T19513	41573-41583	RB	denotes	previously
T19514	41584-41585	-LRB-	denotes	[
T19515	41585-41587	CD	denotes	35
T19516	41587-41588	-RRB-	denotes	]
T19517	41588-41589	.	denotes	.
T19518	41589-41641	sentence	denotes	The Wnt9b riboprobe was provided by T. Carroll [2].
T19519	41590-41593	DT	denotes	The
T19521	41594-41599	NN	denotes	Wnt9b
T19520	41600-41609	NN	denotes	riboprobe
T19523	41610-41613	VBD	denotes	was
T19522	41614-41622	VBN	denotes	provided
T19524	41623-41625	IN	denotes	by
T19525	41626-41628	NNP	denotes	T.
T19526	41629-41636	NNP	denotes	Carroll
T19527	41637-41638	-LRB-	denotes	[
T19528	41638-41639	CD	denotes	2
T19529	41639-41640	-RRB-	denotes	]
T19530	41640-41641	.	denotes	.
T20069	41643-41648	NN	denotes	Pygo1
T20071	41649-41652	CC	denotes	and
T20072	41653-41658	NN	denotes	Pygo2
T20070	41659-41667	NN	denotes	antibody
T20073	41668-41678	NN	denotes	production
T20074	41678-41962	sentence	denotes	To generate anti-human Pygo2 (anti-hPygo2) and anti-mouse Pygo1 (anti-mPygo1) polyclonal antibodies, we subcloned cDNA by PCR corresponding to amino-acid residues 80–327 of human Pygo 2 or amino-acid residues 76–263 of mouse Pygo1 into pGEX4T1 (Amersham Health, Piscataway, NJ, USA).
T20075	41679-41681	TO	denotes	To
T20076	41682-41690	VB	denotes	generate
T20078	41691-41701	JJ	denotes	anti-human
T20079	41702-41707	NN	denotes	Pygo2
T20081	41708-41709	-LRB-	denotes	(
T20082	41709-41720	JJ	denotes	anti-hPygo2
T20083	41720-41721	-RRB-	denotes	)
T20084	41722-41725	CC	denotes	and
T20085	41726-41736	JJ	denotes	anti-mouse
T20086	41737-41742	NN	denotes	Pygo1
T20087	41743-41744	-LRB-	denotes	(
T20088	41744-41755	JJ	denotes	anti-mPygo1
T20089	41755-41756	-RRB-	denotes	)
T20090	41757-41767	JJ	denotes	polyclonal
T20080	41768-41778	NNS	denotes	antibodies
T20091	41778-41780	,	denotes	,
T20092	41780-41782	PRP	denotes	we
T20077	41783-41792	VBD	denotes	subcloned
T20093	41793-41797	NN	denotes	cDNA
T20094	41798-41800	IN	denotes	by
T20095	41801-41804	NN	denotes	PCR
T20096	41805-41818	VBG	denotes	corresponding
T20097	41819-41821	IN	denotes	to
T20098	41822-41827	NN	denotes	amino
T20100	41827-41828	HYPH	denotes	-
T20099	41828-41832	NN	denotes	acid
T20102	41833-41841	NNS	denotes	residues
T20101	41842-41844	CD	denotes	80
T20103	41844-41845	SYM	denotes	–
T20104	41845-41848	CD	denotes	327
T20105	41849-41851	IN	denotes	of
T20106	41852-41857	JJ	denotes	human
T20107	41858-41862	NN	denotes	Pygo
T20108	41863-41864	CD	denotes	2
T20109	41865-41867	CC	denotes	or
T20110	41868-41873	NN	denotes	amino
T20112	41873-41874	HYPH	denotes	-
T20111	41874-41878	NN	denotes	acid
T20114	41879-41887	NNS	denotes	residues
T20113	41888-41890	CD	denotes	76
T20115	41890-41891	SYM	denotes	–
T20116	41891-41894	CD	denotes	263
T20117	41895-41897	IN	denotes	of
T20118	41898-41903	NN	denotes	mouse
T20119	41904-41909	NN	denotes	Pygo1
T20120	41910-41914	IN	denotes	into
T20121	41915-41922	NN	denotes	pGEX4T1
T20122	41923-41924	-LRB-	denotes	(
T20124	41924-41932	NNP	denotes	Amersham
T20123	41933-41939	NNP	denotes	Health
T20125	41939-41941	,	denotes	,
T20126	41941-41951	NNP	denotes	Piscataway
T20127	41951-41953	,	denotes	,
T20128	41953-41955	NNP	denotes	NJ
T20129	41955-41957	,	denotes	,
T20130	41957-41960	NNP	denotes	USA
T20131	41960-41961	-RRB-	denotes	)
T20132	41961-41962	.	denotes	.
T20133	41962-42065	sentence	denotes	The PCR fragments of hPygo 2 and mPygo 1 lack both NHD and PHD conserved regions of hPygo2 and mPygo1.
T20134	41963-41966	DT	denotes	The
T20136	41967-41970	NN	denotes	PCR
T20135	41971-41980	NNS	denotes	fragments
T20138	41981-41983	IN	denotes	of
T20139	41984-41989	NN	denotes	hPygo
T20140	41990-41991	CD	denotes	2
T20141	41992-41995	CC	denotes	and
T20142	41996-42001	NN	denotes	mPygo
T20143	42002-42003	CD	denotes	1
T20137	42004-42008	VBP	denotes	lack
T20144	42009-42013	CC	denotes	both
T20145	42014-42017	NN	denotes	NHD
T20147	42018-42021	CC	denotes	and
T20148	42022-42025	NN	denotes	PHD
T20146	42026-42035	VBN	denotes	conserved
T20149	42036-42043	NNS	denotes	regions
T20150	42044-42046	IN	denotes	of
T20151	42047-42053	NN	denotes	hPygo2
T20152	42054-42057	CC	denotes	and
T20153	42058-42064	NN	denotes	mPygo1
T20154	42064-42065	.	denotes	.
T20155	42065-42235	sentence	denotes	GST-hPygo2 and GST-mPygo1 proteins were expressed in bacteria, purified, and injected into rabbits for antibody production by (Proteintech Group Inc., Chicago, IL, USA).
T20156	42066-42069	NN	denotes	GST
T20158	42069-42070	HYPH	denotes	-
T20157	42070-42076	NN	denotes	hPygo2
T20160	42077-42080	CC	denotes	and
T20161	42081-42084	NN	denotes	GST
T20163	42084-42085	HYPH	denotes	-
T20162	42085-42091	NN	denotes	mPygo1
T20159	42092-42100	NN	denotes	proteins
T20165	42101-42105	VBD	denotes	were
T20164	42106-42115	VBN	denotes	expressed
T20166	42116-42118	IN	denotes	in
T20167	42119-42127	NNS	denotes	bacteria
T20168	42127-42129	,	denotes	,
T20169	42129-42137	VBN	denotes	purified
T20170	42137-42139	,	denotes	,
T20171	42139-42142	CC	denotes	and
T20172	42143-42151	VBN	denotes	injected
T20173	42152-42156	IN	denotes	into
T20174	42157-42164	NNS	denotes	rabbits
T20175	42165-42168	IN	denotes	for
T20176	42169-42177	NN	denotes	antibody
T20177	42178-42188	NN	denotes	production
T20178	42189-42191	IN	denotes	by
T20179	42192-42193	-LRB-	denotes	(
T20180	42193-42204	NNP	denotes	Proteintech
T20182	42205-42210	NNP	denotes	Group
T20181	42211-42215	NNP	denotes	Inc.
T20183	42215-42217	,	denotes	,
T20184	42217-42224	NNP	denotes	Chicago
T20185	42224-42226	,	denotes	,
T20186	42226-42228	NNP	denotes	IL
T20187	42228-42230	,	denotes	,
T20188	42230-42233	NNP	denotes	USA
T20189	42233-42234	-RRB-	denotes	)
T20190	42234-42235	.	denotes	.
T20191	42235-42382	sentence	denotes	The rabbit antisera of anti-mPygo1 and anti-hPygo2 were initially allowed to bind to the GST affinity matrix to remove any antibodies against GST.
T20192	42236-42239	DT	denotes	The
T20194	42240-42246	NN	denotes	rabbit
T20193	42247-42255	NNS	denotes	antisera
T20196	42256-42258	IN	denotes	of
T20197	42259-42270	JJ	denotes	anti-mPygo1
T20198	42271-42274	CC	denotes	and
T20199	42275-42286	JJ	denotes	anti-hPygo2
T20200	42287-42291	VBD	denotes	were
T20201	42292-42301	RB	denotes	initially
T20195	42302-42309	VBN	denotes	allowed
T20202	42310-42312	TO	denotes	to
T20203	42313-42317	VB	denotes	bind
T20204	42318-42320	IN	denotes	to
T20205	42321-42324	DT	denotes	the
T20207	42325-42328	NN	denotes	GST
T20208	42329-42337	NN	denotes	affinity
T20206	42338-42344	NN	denotes	matrix
T20209	42345-42347	TO	denotes	to
T20210	42348-42354	VB	denotes	remove
T20211	42355-42358	DT	denotes	any
T20212	42359-42369	NNS	denotes	antibodies
T20213	42370-42377	IN	denotes	against
T20214	42378-42381	NN	denotes	GST
T20215	42381-42382	.	denotes	.
T20216	42382-42556	sentence	denotes	The anti-hPygo2 and anti-mPygo1 antisera were then separated from the GST affinity matrix and allowed to bind to the GST-hPygo2 or GST-mPygo1 affinity columns, respectively.
T20217	42383-42386	DT	denotes	The
T20219	42387-42398	JJ	denotes	anti-hPygo2
T20220	42399-42402	CC	denotes	and
T20221	42403-42414	JJ	denotes	anti-mPygo1
T20218	42415-42423	NNS	denotes	antisera
T20223	42424-42428	VBD	denotes	were
T20224	42429-42433	RB	denotes	then
T20222	42434-42443	VBN	denotes	separated
T20225	42444-42448	IN	denotes	from
T20226	42449-42452	DT	denotes	the
T20228	42453-42456	NN	denotes	GST
T20229	42457-42465	NN	denotes	affinity
T20227	42466-42472	NN	denotes	matrix
T20230	42473-42476	CC	denotes	and
T20231	42477-42484	VBN	denotes	allowed
T20232	42485-42487	TO	denotes	to
T20233	42488-42492	VB	denotes	bind
T20234	42493-42495	IN	denotes	to
T20235	42496-42499	DT	denotes	the
T20237	42500-42503	NN	denotes	GST
T20239	42503-42504	HYPH	denotes	-
T20238	42504-42510	NN	denotes	hPygo2
T20240	42511-42513	CC	denotes	or
T20241	42514-42517	NN	denotes	GST
T20243	42517-42518	HYPH	denotes	-
T20242	42518-42524	NN	denotes	mPygo1
T20244	42525-42533	NN	denotes	affinity
T20236	42534-42541	NNS	denotes	columns
T20245	42541-42543	,	denotes	,
T20246	42543-42555	RB	denotes	respectively
T20247	42555-42556	.	denotes	.
T20248	42556-42609	sentence	denotes	The bound antibodes were eluted with elution buffer.
T20249	42557-42560	DT	denotes	The
T20251	42561-42566	VBN	denotes	bound
T20250	42567-42576	NNS	denotes	antibodes
T20253	42577-42581	VBD	denotes	were
T20252	42582-42588	VBN	denotes	eluted
T20254	42589-42593	IN	denotes	with
T20255	42594-42601	NN	denotes	elution
T20256	42602-42608	NN	denotes	buffer
T20257	42608-42609	.	denotes	.
T20258	42609-42764	sentence	denotes	To further ensure antibody specficity, the purified antibodies were extensively incubated with Pygo1/2 double-homozygous mutant embryo extract before use.
T20259	42610-42612	TO	denotes	To
T20261	42613-42620	RB	denotes	further
T20260	42621-42627	VB	denotes	ensure
T20263	42628-42636	NN	denotes	antibody
T20264	42637-42647	NN	denotes	specficity
T20265	42647-42649	,	denotes	,
T20266	42649-42652	DT	denotes	the
T20268	42653-42661	VBN	denotes	purified
T20267	42662-42672	NNS	denotes	antibodies
T20269	42673-42677	VBD	denotes	were
T20270	42678-42689	RB	denotes	extensively
T20262	42690-42699	VBN	denotes	incubated
T20271	42700-42704	IN	denotes	with
T20272	42705-42710	NN	denotes	Pygo1
T20274	42710-42711	HYPH	denotes	/
T20275	42711-42712	CD	denotes	2
T20276	42713-42719	JJ	denotes	double
T20277	42719-42720	HYPH	denotes	-
T20273	42720-42730	JJ	denotes	homozygous
T20279	42731-42737	NN	denotes	mutant
T20278	42738-42744	NN	denotes	embryo
T20280	42745-42752	NN	denotes	extract
T20281	42753-42759	IN	denotes	before
T20282	42760-42763	NN	denotes	use
T20283	42763-42764	.	denotes	.
T20633	42766-42769	NN	denotes	BAT
T20635	42769-42770	HYPH	denotes	-
T20634	42770-42773	NN	denotes	gal
T20636	42774-42783	NN	denotes	transgene
T20637	42784-42792	NN	denotes	reporter
T20638	42793-42798	NN	denotes	assay
T20639	42799-42801	IN	denotes	of
T20640	42802-42811	JJ	denotes	canonical
T20642	42812-42815	NN	denotes	Wnt
T20641	42816-42825	NN	denotes	signaling
T20643	42825-42923	sentence	denotes	X-gal staining of both embryos and developing kidneys was performed as previously described [21].
T20644	42826-42827	NN	denotes	X
T20646	42827-42828	HYPH	denotes	-
T20645	42828-42831	NN	denotes	gal
T20647	42832-42840	NN	denotes	staining
T20649	42841-42843	IN	denotes	of
T20650	42844-42848	CC	denotes	both
T20651	42849-42856	NNS	denotes	embryos
T20652	42857-42860	CC	denotes	and
T20653	42861-42871	VBG	denotes	developing
T20654	42872-42879	NNS	denotes	kidneys
T20655	42880-42883	VBD	denotes	was
T20648	42884-42893	VBN	denotes	performed
T20656	42894-42896	IN	denotes	as
T20658	42897-42907	RB	denotes	previously
T20657	42908-42917	VBN	denotes	described
T20659	42918-42919	-LRB-	denotes	[
T20660	42919-42921	CD	denotes	21
T20661	42921-42922	-RRB-	denotes	]
T20662	42922-42923	.	denotes	.
T20663	42923-43069	sentence	denotes	Care was taken to reduce endogenous β-galactosidase activity within the developing kidney by increasing pH of the X-gal staining solution to 8.0.
T20664	42924-42928	NN	denotes	Care
T20666	42929-42932	VBD	denotes	was
T20665	42933-42938	VBN	denotes	taken
T20667	42939-42941	TO	denotes	to
T20668	42942-42948	VB	denotes	reduce
T20669	42949-42959	JJ	denotes	endogenous
T20671	42960-42961	NN	denotes	β
T20673	42961-42962	HYPH	denotes	-
T20672	42962-42975	NN	denotes	galactosidase
T20670	42976-42984	NN	denotes	activity
T20674	42985-42991	IN	denotes	within
T20675	42992-42995	DT	denotes	the
T20677	42996-43006	VBG	denotes	developing
T20676	43007-43013	NN	denotes	kidney
T20678	43014-43016	IN	denotes	by
T20679	43017-43027	VBG	denotes	increasing
T20680	43028-43030	NN	denotes	pH
T20681	43031-43033	IN	denotes	of
T20682	43034-43037	DT	denotes	the
T20684	43038-43039	NN	denotes	X
T20686	43039-43040	HYPH	denotes	-
T20685	43040-43043	NN	denotes	gal
T20687	43044-43052	NN	denotes	staining
T20683	43053-43061	NN	denotes	solution
T20688	43062-43064	IN	denotes	to
T20689	43065-43068	CD	denotes	8.0
T20690	43068-43069	.	denotes	.
T20691	43069-43243	sentence	denotes	Changes in transgene β-Gal expression were quantitated using a β-Gal ELISA (Enzyme-linked immunoassay) kit (Roche, Indianapolis, IN), normalizing according to total protein.
T20692	43070-43077	NNS	denotes	Changes
T20694	43078-43080	IN	denotes	in
T20695	43081-43090	NN	denotes	transgene
T20697	43091-43092	NN	denotes	β
T20698	43092-43093	HYPH	denotes	-
T20696	43093-43096	NN	denotes	Gal
T20699	43097-43107	NN	denotes	expression
T20700	43108-43112	VBD	denotes	were
T20693	43113-43124	VBN	denotes	quantitated
T20701	43125-43130	VBG	denotes	using
T20702	43131-43132	DT	denotes	a
T20704	43133-43134	NN	denotes	β
T20706	43134-43135	HYPH	denotes	-
T20705	43135-43138	NN	denotes	Gal
T20707	43139-43144	NNP	denotes	ELISA
T20708	43145-43146	-LRB-	denotes	(
T20709	43146-43152	NN	denotes	Enzyme
T20711	43152-43153	HYPH	denotes	-
T20710	43153-43159	VBN	denotes	linked
T20712	43160-43171	NN	denotes	immunoassay
T20713	43171-43172	-RRB-	denotes	)
T20703	43173-43176	NN	denotes	kit
T20714	43177-43178	-LRB-	denotes	(
T20715	43178-43183	NNP	denotes	Roche
T20716	43183-43185	,	denotes	,
T20717	43185-43197	NNP	denotes	Indianapolis
T20718	43197-43199	,	denotes	,
T20719	43199-43201	NNP	denotes	IN
T20720	43201-43202	-RRB-	denotes	)
T20721	43202-43204	,	denotes	,
T20722	43204-43215	VBG	denotes	normalizing
T20723	43216-43225	VBG	denotes	according
T20724	43226-43228	IN	denotes	to
T20725	43229-43234	JJ	denotes	total
T20726	43235-43242	NN	denotes	protein
T20727	43242-43243	.	denotes	.
T20728	43243-43428	sentence	denotes	Each genotype was represented by a sample size of 4 except the non-transgenic (n = 5), Pygo1+/- ; Pygo2+/+ (n = 6), Pygo1-/- ; Pygo2+/- (n = 8), and Pygo1-/- ; Pygo2-/- (n = 6) groups.
T20729	43244-43248	DT	denotes	Each
T20730	43249-43257	NN	denotes	genotype
T20732	43258-43261	VBD	denotes	was
T20731	43262-43273	VBN	denotes	represented
T20733	43274-43276	IN	denotes	by
T20734	43277-43278	DT	denotes	a
T20736	43279-43285	NN	denotes	sample
T20735	43286-43290	NN	denotes	size
T20737	43291-43293	IN	denotes	of
T20738	43294-43295	CD	denotes	4
T20739	43296-43302	IN	denotes	except
T20740	43303-43306	DT	denotes	the
T20742	43307-43321	JJ	denotes	non-transgenic
T20743	43322-43323	-LRB-	denotes	(
T20745	43323-43324	NN	denotes	n
T20746	43325-43326	SYM	denotes	=
T20744	43327-43328	CD	denotes	5
T20747	43328-43329	-RRB-	denotes	)
T20748	43329-43331	,	denotes	,
T20749	43331-43336	NN	denotes	Pygo1
T20750	43336-43337	SYM	denotes	+
T20751	43337-43338	HYPH	denotes	/
T20752	43338-43339	SYM	denotes	-
T20753	43340-43341	:	denotes	;
T20754	43342-43347	NN	denotes	Pygo2
T20755	43347-43348	SYM	denotes	+
T20756	43348-43349	HYPH	denotes	/
T20757	43349-43350	SYM	denotes	+
T20758	43351-43352	-LRB-	denotes	(
T20760	43352-43353	NN	denotes	n
T20761	43354-43355	SYM	denotes	=
T20759	43356-43357	CD	denotes	6
T20762	43357-43358	-RRB-	denotes	)
T20763	43358-43360	,	denotes	,
T20764	43360-43365	NN	denotes	Pygo1
T20765	43365-43366	SYM	denotes	-
T20766	43366-43367	HYPH	denotes	/
T20767	43367-43368	SYM	denotes	-
T20768	43369-43370	:	denotes	;
T20769	43371-43376	NN	denotes	Pygo2
T20770	43376-43377	SYM	denotes	+
T20771	43377-43378	HYPH	denotes	/
T20772	43378-43379	SYM	denotes	-
T20773	43380-43381	-LRB-	denotes	(
T20775	43381-43382	NN	denotes	n
T20776	43383-43384	SYM	denotes	=
T20774	43385-43386	CD	denotes	8
T20777	43386-43387	-RRB-	denotes	)
T20778	43387-43389	,	denotes	,
T20779	43389-43392	CC	denotes	and
T20780	43393-43398	NN	denotes	Pygo1
T20781	43398-43399	SYM	denotes	-
T20782	43399-43400	HYPH	denotes	/
T20783	43400-43401	SYM	denotes	-
T20784	43402-43403	:	denotes	;
T20785	43404-43409	NN	denotes	Pygo2
T20786	43409-43410	SYM	denotes	-
T20787	43410-43411	HYPH	denotes	/
T20788	43411-43412	SYM	denotes	-
T20789	43413-43414	-LRB-	denotes	(
T20791	43414-43415	NN	denotes	n
T20792	43416-43417	SYM	denotes	=
T20790	43418-43419	CD	denotes	6
T20793	43419-43420	-RRB-	denotes	)
T20741	43421-43427	NNS	denotes	groups
T20794	43427-43428	.	denotes	.
T21192	43430-43440	NN	denotes	Microarray
T21193	43441-43449	NN	denotes	analysis
T21194	43449-43636	sentence	denotes	Total RNA was isolated from E18.5 kidneys dissected from normal and Pygo1/Pygo2 compound null embryos using a commercial kit (Stratagene Absolutely RNA Microprep Kit; La Jolla, CA, USA).
T21195	43450-43455	JJ	denotes	Total
T21196	43456-43459	NN	denotes	RNA
T21198	43460-43463	VBD	denotes	was
T21197	43464-43472	VBN	denotes	isolated
T21199	43473-43477	IN	denotes	from
T21200	43478-43483	NN	denotes	E18.5
T21201	43484-43491	NNS	denotes	kidneys
T21202	43492-43501	VBN	denotes	dissected
T21203	43502-43506	IN	denotes	from
T21204	43507-43513	JJ	denotes	normal
T21206	43514-43517	CC	denotes	and
T21207	43518-43523	NN	denotes	Pygo1
T21209	43523-43524	HYPH	denotes	/
T21208	43524-43529	NN	denotes	Pygo2
T21211	43530-43538	NN	denotes	compound
T21210	43539-43543	JJ	denotes	null
T21205	43544-43551	NNS	denotes	embryos
T21212	43552-43557	VBG	denotes	using
T21213	43558-43559	DT	denotes	a
T21215	43560-43570	JJ	denotes	commercial
T21214	43571-43574	NN	denotes	kit
T21216	43575-43576	-LRB-	denotes	(
T21217	43576-43586	NNP	denotes	Stratagene
T21219	43587-43597	NNP	denotes	Absolutely
T21220	43598-43601	NNP	denotes	RNA
T21221	43602-43611	NNP	denotes	Microprep
T21218	43612-43615	NN	denotes	Kit
T21222	43615-43616	:	denotes	;
T21224	43617-43619	NNP	denotes	La
T21225	43620-43625	NNP	denotes	Jolla
T21226	43625-43627	,	denotes	,
T21227	43627-43629	NNP	denotes	CA
T21228	43629-43631	,	denotes	,
T21223	43631-43634	NNP	denotes	USA
T21229	43634-43635	-RRB-	denotes	)
T21230	43635-43636	.	denotes	.
T21231	43636-43787	sentence	denotes	An aliquot (300 ng) of total RNA was processed and labeled using a commercial kit (TargetAmp 1-Round Aminoallyl-aRNA Ki; Epicentre, Madison, WI, USA).
T21232	43637-43639	DT	denotes	An
T21233	43640-43647	NN	denotes	aliquot
T21235	43648-43649	-LRB-	denotes	(
T21237	43649-43652	CD	denotes	300
T21236	43653-43655	NN	denotes	ng
T21238	43655-43656	-RRB-	denotes	)
T21239	43657-43659	IN	denotes	of
T21240	43660-43665	JJ	denotes	total
T21241	43666-43669	NN	denotes	RNA
T21242	43670-43673	VBD	denotes	was
T21234	43674-43683	VBN	denotes	processed
T21243	43684-43687	CC	denotes	and
T21244	43688-43695	VBN	denotes	labeled
T21245	43696-43701	VBG	denotes	using
T21246	43702-43703	DT	denotes	a
T21248	43704-43714	JJ	denotes	commercial
T21247	43715-43718	NN	denotes	kit
T21249	43719-43720	-LRB-	denotes	(
T21250	43720-43729	NNP	denotes	TargetAmp
T21252	43730-43731	CD	denotes	1
T21254	43731-43732	HYPH	denotes	-
T21253	43732-43737	JJ	denotes	Round
T21255	43738-43748	NN	denotes	Aminoallyl
T21257	43748-43749	HYPH	denotes	-
T21256	43749-43753	NN	denotes	aRNA
T21251	43754-43756	NN	denotes	Ki
T21258	43756-43757	:	denotes	;
T21259	43758-43767	NNP	denotes	Epicentre
T21260	43767-43769	,	denotes	,
T21261	43769-43776	NNP	denotes	Madison
T21262	43776-43778	,	denotes	,
T21263	43778-43780	NNP	denotes	WI
T21264	43780-43782	,	denotes	,
T21265	43782-43785	NNP	denotes	USA
T21266	43785-43786	-RRB-	denotes	)
T21267	43786-43787	.	denotes	.
T21268	43787-43989	sentence	denotes	Labeled RNA was hybridized to microarrays (Sentrix Mouse-6 expression Beadchip; Illumina, San Diego, CA) providing coverage of over 47000 genes and expressed sequence tags as previously described [36].
T21269	43788-43795	VBN	denotes	Labeled
T21270	43796-43799	NN	denotes	RNA
T21272	43800-43803	VBD	denotes	was
T21271	43804-43814	VBN	denotes	hybridized
T21273	43815-43817	IN	denotes	to
T21274	43818-43829	NNS	denotes	microarrays
T21275	43830-43831	-LRB-	denotes	(
T21276	43831-43838	NNP	denotes	Sentrix
T21278	43839-43844	NNP	denotes	Mouse
T21279	43844-43845	HYPH	denotes	-
T21280	43845-43846	CD	denotes	6
T21281	43847-43857	NN	denotes	expression
T21277	43858-43866	NN	denotes	Beadchip
T21282	43866-43867	:	denotes	;
T21283	43868-43876	NNP	denotes	Illumina
T21284	43876-43878	,	denotes	,
T21285	43878-43881	NNP	denotes	San
T21286	43882-43887	NNP	denotes	Diego
T21287	43887-43889	,	denotes	,
T21288	43889-43891	NNP	denotes	CA
T21289	43891-43892	-RRB-	denotes	)
T21290	43893-43902	VBG	denotes	providing
T21291	43903-43911	NN	denotes	coverage
T21292	43912-43914	IN	denotes	of
T21293	43915-43919	IN	denotes	over
T21294	43920-43925	CD	denotes	47000
T21295	43926-43931	NNS	denotes	genes
T21296	43932-43935	CC	denotes	and
T21297	43936-43945	VBD	denotes	expressed
T21298	43946-43954	NN	denotes	sequence
T21299	43955-43959	NNS	denotes	tags
T21300	43960-43962	IN	denotes	as
T21302	43963-43973	RB	denotes	previously
T21301	43974-43983	VBN	denotes	described
T21303	43984-43985	-LRB-	denotes	[
T21304	43985-43987	CD	denotes	36
T21305	43987-43988	-RRB-	denotes	]
T21306	43988-43989	.	denotes	.
T21307	43989-44098	sentence	denotes	Microarray analysis of Pygo1/Pygo2 null and normal wild-type kidneys was performed in biological triplicate.
T21308	43990-44000	NN	denotes	Microarray
T21309	44001-44009	NN	denotes	analysis
T21311	44010-44012	IN	denotes	of
T21312	44013-44018	NN	denotes	Pygo1
T21314	44018-44019	HYPH	denotes	/
T21313	44019-44024	NN	denotes	Pygo2
T21315	44025-44029	JJ	denotes	null
T21316	44030-44033	CC	denotes	and
T21317	44034-44040	JJ	denotes	normal
T21319	44041-44045	JJ	denotes	wild
T21320	44045-44046	HYPH	denotes	-
T21318	44046-44050	NN	denotes	type
T21321	44051-44058	NNS	denotes	kidneys
T21322	44059-44062	VBD	denotes	was
T21310	44063-44072	VBN	denotes	performed
T21323	44073-44075	IN	denotes	in
T21324	44076-44086	JJ	denotes	biological
T21325	44087-44097	NN	denotes	triplicate
T21326	44097-44098	.	denotes	.
T21327	44098-44280	sentence	denotes	Raw signal intensities of each probe were obtained from data analysis software (Beadstudio ;Illumina) and imported into GeneSpring GX 7.3 (Agilent Technologies, Palo Alto, CA, USA).
T21328	44099-44102	JJ	denotes	Raw
T21330	44103-44109	NN	denotes	signal
T21329	44110-44121	NNS	denotes	intensities
T21332	44122-44124	IN	denotes	of
T21333	44125-44129	DT	denotes	each
T21334	44130-44135	NN	denotes	probe
T21335	44136-44140	VBD	denotes	were
T21331	44141-44149	VBN	denotes	obtained
T21336	44150-44154	IN	denotes	from
T21337	44155-44159	NNS	denotes	data
T21339	44160-44168	NN	denotes	analysis
T21338	44169-44177	NN	denotes	software
T21340	44178-44179	-LRB-	denotes	(
T21341	44179-44189	NNP	denotes	Beadstudio
T21342	44190-44191	:	denotes	;
T21343	44191-44199	NNP	denotes	Illumina
T21344	44199-44200	-RRB-	denotes	)
T21345	44201-44204	CC	denotes	and
T21346	44205-44213	VBN	denotes	imported
T21347	44214-44218	IN	denotes	into
T21348	44219-44229	NNP	denotes	GeneSpring
T21349	44230-44232	NNP	denotes	GX
T21350	44233-44236	CD	denotes	7.3
T21351	44237-44238	-LRB-	denotes	(
T21353	44238-44245	NNP	denotes	Agilent
T21352	44246-44258	NNP	denotes	Technologies
T21354	44258-44260	,	denotes	,
T21355	44260-44264	NNP	denotes	Palo
T21356	44265-44269	NNP	denotes	Alto
T21357	44269-44271	,	denotes	,
T21358	44271-44273	NNP	denotes	CA
T21359	44273-44275	,	denotes	,
T21360	44275-44278	NNP	denotes	USA
T21361	44278-44279	-RRB-	denotes	)
T21362	44279-44280	.	denotes	.
T21363	44280-44405	sentence	denotes	Genes were selected on the basis of greater than two-fold average fold change and statistical significance (p-value < 0.05).
T21364	44281-44286	NNS	denotes	Genes
T21366	44287-44291	VBD	denotes	were
T21365	44292-44300	VBN	denotes	selected
T21367	44301-44303	IN	denotes	on
T21368	44304-44307	DT	denotes	the
T21369	44308-44313	NN	denotes	basis
T21370	44314-44316	IN	denotes	of
T21371	44317-44324	JJR	denotes	greater
T21373	44325-44329	IN	denotes	than
T21372	44330-44338	RB	denotes	two-fold
T21375	44339-44346	JJ	denotes	average
T21376	44347-44351	NN	denotes	fold
T21374	44352-44358	NN	denotes	change
T21377	44359-44362	CC	denotes	and
T21378	44363-44374	JJ	denotes	statistical
T21379	44375-44387	NN	denotes	significance
T21380	44388-44389	-LRB-	denotes	(
T21382	44389-44390	NN	denotes	p
T21384	44390-44391	HYPH	denotes	-
T21383	44391-44396	NN	denotes	value
T21385	44397-44398	SYM	denotes	<
T21381	44399-44403	CD	denotes	0.05
T21386	44403-44404	-RRB-	denotes	)
T21387	44404-44405	.	denotes	.
T21388	44405-44464	sentence	denotes	Previously described Wnt target genes were obtained from .
T21389	44406-44416	RB	denotes	Previously
T21390	44417-44426	VBN	denotes	described
T21392	44427-44430	NN	denotes	Wnt
T21393	44431-44437	NN	denotes	target
T21391	44438-44443	NNS	denotes	genes
T21395	44444-44448	VBD	denotes	were
T21394	44449-44457	VBN	denotes	obtained
T21396	44458-44462	IN	denotes	from
T21397	44463-44464	.	denotes	.
T21853	44466-44478	JJ	denotes	Quantitative
T21855	44479-44482	NN	denotes	PCR
T21854	44483-44493	NN	denotes	validation
T21856	44494-44496	IN	denotes	of
T21857	44497-44507	NN	denotes	microarray
T21858	44508-44515	NNS	denotes	results
T21859	44515-44788	sentence	denotes	Total RNA from E18.5 Pygo1/Pygo2 null and control kidneys (both represented in duplicate and distinct from the kidneys used for microrray analysis) was purified using a commercial kit (Absolutely RNA Microprep Kit; Stratagene, La Jolla, CA USA) including DNAse1 treatment.
T21860	44516-44521	JJ	denotes	Total
T21861	44522-44525	NN	denotes	RNA
T21863	44526-44530	IN	denotes	from
T21864	44531-44536	NN	denotes	E18.5
T21866	44537-44542	NN	denotes	Pygo1
T21868	44542-44543	HYPH	denotes	/
T21867	44543-44548	NN	denotes	Pygo2
T21869	44549-44553	JJ	denotes	null
T21870	44554-44557	CC	denotes	and
T21871	44558-44565	NN	denotes	control
T21865	44566-44573	NNS	denotes	kidneys
T21872	44574-44575	-LRB-	denotes	(
T21873	44575-44579	CC	denotes	both
T21874	44580-44591	VBN	denotes	represented
T21875	44592-44594	IN	denotes	in
T21876	44595-44604	NN	denotes	duplicate
T21877	44605-44608	CC	denotes	and
T21878	44609-44617	JJ	denotes	distinct
T21879	44618-44622	IN	denotes	from
T21880	44623-44626	DT	denotes	the
T21881	44627-44634	NNS	denotes	kidneys
T21882	44635-44639	VBN	denotes	used
T21883	44640-44643	IN	denotes	for
T21884	44644-44653	NN	denotes	microrray
T21885	44654-44662	NN	denotes	analysis
T21886	44662-44663	-RRB-	denotes	)
T21887	44664-44667	VBD	denotes	was
T21862	44668-44676	VBN	denotes	purified
T21888	44677-44682	VBG	denotes	using
T21889	44683-44684	DT	denotes	a
T21891	44685-44695	JJ	denotes	commercial
T21890	44696-44699	NN	denotes	kit
T21892	44700-44701	-LRB-	denotes	(
T21893	44701-44711	NNP	denotes	Absolutely
T21895	44712-44715	NNP	denotes	RNA
T21896	44716-44725	NN	denotes	Microprep
T21894	44726-44729	NN	denotes	Kit
T21897	44729-44730	:	denotes	;
T21898	44731-44741	NNP	denotes	Stratagene
T21899	44741-44743	,	denotes	,
T21900	44743-44745	NNP	denotes	La
T21901	44746-44751	NNP	denotes	Jolla
T21902	44751-44753	,	denotes	,
T21903	44753-44755	NNP	denotes	CA
T21904	44756-44759	NNP	denotes	USA
T21905	44759-44760	-RRB-	denotes	)
T21906	44761-44770	VBG	denotes	including
T21907	44771-44777	NN	denotes	DNAse1
T21908	44778-44787	NN	denotes	treatment
T21909	44787-44788	.	denotes	.
T21910	44788-44898	sentence	denotes	cDNA was generated using random hexamers according to conventional protocols (Invitrogen, Carlsbad, CA, USA).
T21911	44789-44793	NN	denotes	cDNA
T21913	44794-44797	VBD	denotes	was
T21912	44798-44807	VBN	denotes	generated
T21914	44808-44813	VBG	denotes	using
T21915	44814-44820	JJ	denotes	random
T21916	44821-44829	NNS	denotes	hexamers
T21917	44830-44839	VBG	denotes	according
T21918	44840-44842	IN	denotes	to
T21919	44843-44855	JJ	denotes	conventional
T21920	44856-44865	NNS	denotes	protocols
T21921	44866-44867	-LRB-	denotes	(
T21922	44867-44877	NNP	denotes	Invitrogen
T21923	44877-44879	,	denotes	,
T21924	44879-44887	NNP	denotes	Carlsbad
T21925	44887-44889	,	denotes	,
T21926	44889-44891	NNP	denotes	CA
T21927	44891-44893	,	denotes	,
T21928	44893-44896	NNP	denotes	USA
T21929	44896-44897	-RRB-	denotes	)
T21930	44897-44898	.	denotes	.
T21931	44898-45498	sentence	denotes	The following primers were generated to include the sequence obtained from the Illumina probe: Actb (TTGCTGACAGGATGCAGAAG, ACATCTGCTGGAAGGTGGAC); Aldh1a7 (CCAGAAAGTGGTGTTTTGCT, GAGTTACAGAGAGCTTGCACCTT); Col8a1 (GCAGACAGGCATCTTCACCT, TGTGTACATCATGGGCTCGT); Csrp1 (CAGCATAGCCCAGGGTAGAG, TGGGCAAGGTAGTGAAGGTT); Klk5 (GCAGCATTACACCCGTCATA, TTGCCTCCATCCATATCTCC); Picalm (GGGAGGGAACAGAAATCCTT, GCACCGATCAACAGTGCAG); Pygo2 (TTCTGGGAACTTGTGCACTG, AACTTCCTCCAGCCCATTTT); Ren2 (TTGTGAGCTGTAGCCAGGTG, TGTGCACAGCTTGTCTCTCC) and Tia1 (TGATTGAAGGGCTACTAGAGTGGT, AGCCCCAGAGGACAATTTTT) using Primer3 software [37].
T21932	44899-44902	DT	denotes	The
T21934	44903-44912	VBG	denotes	following
T21933	44913-44920	NNS	denotes	primers
T21936	44921-44925	VBD	denotes	were
T21935	44926-44935	VBN	denotes	generated
T21937	44936-44938	TO	denotes	to
T21938	44939-44946	VB	denotes	include
T21939	44947-44950	DT	denotes	the
T21940	44951-44959	NN	denotes	sequence
T21941	44960-44968	VBN	denotes	obtained
T21942	44969-44973	IN	denotes	from
T21943	44974-44977	DT	denotes	the
T21945	44978-44986	NNP	denotes	Illumina
T21944	44987-44992	NN	denotes	probe
T21946	44992-44994	:	denotes	:
T21947	44994-44998	NN	denotes	Actb
T21948	44999-45000	-LRB-	denotes	(
T21950	45000-45020	NN	denotes	TTGCTGACAGGATGCAGAAG
T21951	45020-45022	,	denotes	,
T21949	45022-45042	NN	denotes	ACATCTGCTGGAAGGTGGAC
T21952	45042-45043	-RRB-	denotes	)
T21953	45043-45044	:	denotes	;
T21954	45045-45052	NN	denotes	Aldh1a7
T21955	45053-45054	-LRB-	denotes	(
T21957	45054-45074	NN	denotes	CCAGAAAGTGGTGTTTTGCT
T21958	45074-45076	,	denotes	,
T21956	45076-45099	NN	denotes	GAGTTACAGAGAGCTTGCACCTT
T21959	45099-45100	-RRB-	denotes	)
T21960	45100-45101	:	denotes	;
T21961	45102-45108	NN	denotes	Col8a1
T21962	45109-45110	-LRB-	denotes	(
T21964	45110-45130	NN	denotes	GCAGACAGGCATCTTCACCT
T21965	45130-45132	,	denotes	,
T21963	45132-45152	NN	denotes	TGTGTACATCATGGGCTCGT
T21966	45152-45153	-RRB-	denotes	)
T21967	45153-45154	:	denotes	;
T21968	45155-45160	NN	denotes	Csrp1
T21969	45161-45162	-LRB-	denotes	(
T21971	45162-45182	NN	denotes	CAGCATAGCCCAGGGTAGAG
T21972	45182-45184	,	denotes	,
T21970	45184-45204	NN	denotes	TGGGCAAGGTAGTGAAGGTT
T21973	45204-45205	-RRB-	denotes	)
T21974	45205-45206	:	denotes	;
T21975	45207-45211	NN	denotes	Klk5
T21976	45212-45213	-LRB-	denotes	(
T21978	45213-45233	NN	denotes	GCAGCATTACACCCGTCATA
T21979	45233-45235	,	denotes	,
T21977	45235-45255	NN	denotes	TTGCCTCCATCCATATCTCC
T21980	45255-45256	-RRB-	denotes	)
T21981	45256-45257	:	denotes	;
T21982	45258-45264	NN	denotes	Picalm
T21983	45265-45266	-LRB-	denotes	(
T21985	45266-45286	NN	denotes	GGGAGGGAACAGAAATCCTT
T21986	45286-45288	,	denotes	,
T21984	45288-45307	NN	denotes	GCACCGATCAACAGTGCAG
T21987	45307-45308	-RRB-	denotes	)
T21988	45308-45309	:	denotes	;
T21989	45310-45315	NN	denotes	Pygo2
T21990	45316-45317	-LRB-	denotes	(
T21992	45317-45337	NN	denotes	TTCTGGGAACTTGTGCACTG
T21993	45337-45339	,	denotes	,
T21991	45339-45359	NN	denotes	AACTTCCTCCAGCCCATTTT
T21994	45359-45360	-RRB-	denotes	)
T21995	45360-45361	:	denotes	;
T21996	45362-45366	NN	denotes	Ren2
T21997	45367-45368	-LRB-	denotes	(
T21999	45368-45388	NN	denotes	TTGTGAGCTGTAGCCAGGTG
T22000	45388-45390	,	denotes	,
T21998	45390-45410	NN	denotes	TGTGCACAGCTTGTCTCTCC
T22001	45410-45411	-RRB-	denotes	)
T22002	45412-45415	CC	denotes	and
T22003	45416-45420	NN	denotes	Tia1
T22004	45421-45422	-LRB-	denotes	(
T22006	45422-45446	NN	denotes	TGATTGAAGGGCTACTAGAGTGGT
T22007	45446-45448	,	denotes	,
T22005	45448-45468	NN	denotes	AGCCCCAGAGGACAATTTTT
T22008	45468-45469	-RRB-	denotes	)
T22009	45470-45475	VBG	denotes	using
T22010	45476-45483	NN	denotes	Primer3
T22011	45484-45492	NN	denotes	software
T22012	45493-45494	-LRB-	denotes	[
T22013	45494-45496	CD	denotes	37
T22014	45496-45497	-RRB-	denotes	]
T22015	45497-45498	.	denotes	.
T22016	45498-45656	sentence	denotes	Relative quantitative PCR was performed according to the conventional SYBR Green protocol (Stratagene) using a quantitative PCR system (Mx3000p; Stratagene).
T22017	45499-45507	JJ	denotes	Relative
T22019	45508-45520	JJ	denotes	quantitative
T22018	45521-45524	NN	denotes	PCR
T22021	45525-45528	VBD	denotes	was
T22020	45529-45538	VBN	denotes	performed
T22022	45539-45548	VBG	denotes	according
T22023	45549-45551	IN	denotes	to
T22024	45552-45555	DT	denotes	the
T22026	45556-45568	JJ	denotes	conventional
T22027	45569-45573	NNP	denotes	SYBR
T22028	45574-45579	NNP	denotes	Green
T22025	45580-45588	NN	denotes	protocol
T22029	45589-45590	-LRB-	denotes	(
T22030	45590-45600	NNP	denotes	Stratagene
T22031	45600-45601	-RRB-	denotes	)
T22032	45602-45607	VBG	denotes	using
T22033	45608-45609	DT	denotes	a
T22035	45610-45622	JJ	denotes	quantitative
T22036	45623-45626	NN	denotes	PCR
T22034	45627-45633	NN	denotes	system
T22037	45634-45635	-LRB-	denotes	(
T22039	45635-45642	NN	denotes	Mx3000p
T22040	45642-45643	:	denotes	;
T22038	45644-45654	NNP	denotes	Stratagene
T22041	45654-45655	-RRB-	denotes	)
T22042	45655-45656	.	denotes	.
T22043	45656-45768	sentence	denotes	Dissociation curve and agarose-gel analysis of each primer set were used to insure specificity of the amplicon.
T22044	45657-45669	NN	denotes	Dissociation
T22045	45670-45675	NN	denotes	curve
T22047	45676-45679	CC	denotes	and
T22048	45680-45687	NN	denotes	agarose
T22050	45687-45688	HYPH	denotes	-
T22049	45688-45691	NN	denotes	gel
T22046	45692-45700	NN	denotes	analysis
T22052	45701-45703	IN	denotes	of
T22053	45704-45708	DT	denotes	each
T22054	45709-45715	NN	denotes	primer
T22055	45716-45719	VBN	denotes	set
T22056	45720-45724	VBD	denotes	were
T22051	45725-45729	VBN	denotes	used
T22057	45730-45732	TO	denotes	to
T22058	45733-45739	VB	denotes	insure
T22059	45740-45751	NN	denotes	specificity
T22060	45752-45754	IN	denotes	of
T22061	45755-45758	DT	denotes	the
T22062	45759-45767	NN	denotes	amplicon
T22063	45767-45768	.	denotes	.
T22064	45768-45893	sentence	denotes	All data were normalized to an internal housekeeping control (Actb) and analyzed using the 2(-Delta Delta C(T)) method [38].
T22065	45769-45772	DT	denotes	All
T22066	45773-45777	NNS	denotes	data
T22068	45778-45782	VBD	denotes	were
T22067	45783-45793	VBN	denotes	normalized
T22069	45794-45796	IN	denotes	to
T22070	45797-45799	DT	denotes	an
T22072	45800-45808	JJ	denotes	internal
T22073	45809-45821	NN	denotes	housekeeping
T22071	45822-45829	NN	denotes	control
T22074	45830-45831	-LRB-	denotes	(
T22075	45831-45835	NN	denotes	Actb
T22076	45835-45836	-RRB-	denotes	)
T22077	45837-45840	CC	denotes	and
T22078	45841-45849	VBN	denotes	analyzed
T22079	45850-45855	VBG	denotes	using
T22080	45856-45859	DT	denotes	the
T22082	45860-45861	CD	denotes	2
T22084	45861-45862	-LRB-	denotes	(
T22085	45862-45863	HYPH	denotes	-
T22086	45863-45868	NN	denotes	Delta
T22087	45869-45874	NN	denotes	Delta
T22088	45875-45876	NN	denotes	C
T22089	45876-45877	-LRB-	denotes	(
T22083	45877-45878	NN	denotes	T
T22090	45878-45879	-RRB-	denotes	)
T22091	45879-45880	-RRB-	denotes	)
T22081	45881-45887	NN	denotes	method
T22092	45888-45889	-LRB-	denotes	[
T22093	45889-45891	CD	denotes	38
T22094	45891-45892	-RRB-	denotes	]
T22095	45892-45893	.	denotes	.
R10	T949	T943	prep	in,roles
R100	T1052	T1045	prep	after,died
R1000	T3574	T3575	det	both,genes
R1001	T3575	T3573	pobj	genes,for
R1002	T3576	T3571	punct	", ",sequences
R1003	T3577	T3571	prep	including,sequences
R1004	T3578	T3579	nummod	89,%
R1005	T3579	T3577	pobj	%,including
R1006	T3580	T3579	prep	of,%
R1007	T3581	T3582	compound	coding,sequence
R1008	T3582	T3580	pobj	sequence,of
R1009	T3583	T3582	prep	for,sequence
R101	T1053	T1052	pobj	birth,after
R1010	T3584	T3583	pobj	Pygo1,for
R1011	T3585	T3579	cc	and,%
R1012	T3586	T3587	nummod	87,%
R1013	T3587	T3579	conj	%,%
R1014	T3588	T3587	prep	of,%
R1015	T3589	T3590	compound	coding,sequence
R1016	T3590	T3588	pobj	sequence,of
R1017	T3591	T3590	prep	for,sequence
R1018	T3592	T3591	pobj	Pygo2,for
R1019	T3593	T3563	punct	.,confirmed
R102	T1054	T1045	punct	", ",died
R1020	T3595	T3596	amod	Previous,studies
R1021	T3596	T3597	nsubj	studies,shown
R1022	T3598	T3596	prep	in,studies
R1023	T3599	T3598	pobj	Drosophila,in
R1024	T3600	T3597	aux	have,shown
R1025	T3601	T3602	mark	that,eliminate
R1026	T3602	T3597	ccomp	eliminate,shown
R1027	T3603	T3604	advmod	even,mutation
R1028	T3604	T3602	nsubj	mutation,eliminate
R1029	T3605	T3604	det	a,mutation
R103	T1055	T1045	prep	with,died
R1030	T3606	T3604	amod	single,mutation
R1031	T3607	T3604	compound	missense,mutation
R1032	T3608	T3604	prep	in,mutation
R1033	T3609	T3610	det	the,domain
R1034	T3610	T3608	pobj	domain,in
R1035	T3611	T3610	compound	PHD,domain
R1036	T3612	T3602	aux	can,eliminate
R1037	T3613	T3614	compound	Pygo,function
R1038	T3614	T3602	dobj	function,eliminate
R1039	T3615	T3602	prep	in,eliminate
R104	T1056	T1057	det	a,phenotype
R1040	T3616	T3617	compound	Wnt,signaling
R1041	T3617	T3615	pobj	signaling,in
R1042	T3618	T3619	punct	[,8
R1043	T3619	T3597	parataxis	8,shown
R1044	T3620	T3619	punct	],8
R1045	T3621	T3597	punct	.,shown
R1046	T4091	T4092	nmod	Pygo1,mutant
R1047	T4092	T4095	compound	mutant,phenotypes
R1048	T4093	T4091	cc	and,Pygo1
R1049	T4094	T4091	conj	Pygo2,Pygo1
R105	T1057	T1055	pobj	phenotype,with
R1050	T4097	T4098	npadvmod	Pygo1,null
R1051	T4098	T4100	amod	null,mice
R1052	T4099	T4098	amod	homozygous,null
R1053	T4100	T4101	nsubj	mice,were
R1054	T4102	T4101	acomp	viable,were
R1055	T4103	T4102	cc	and,viable
R1056	T4104	T4102	conj	fertile,viable
R1057	T4105	T4106	mark	with,detectable
R1058	T4106	T4101	advcl	detectable,were
R1059	T4107	T4108	det	no,defects
R106	T1058	T1057	prep	including,phenotype
R1060	T4108	T4106	nsubj	defects,detectable
R1061	T4109	T4108	amod	developmental,defects
R1062	T4110	T4101	punct	.,were
R1063	T4112	T4113	nsubj	This,was
R1064	T4114	T4113	acomp	surprising,was
R1065	T4115	T4113	prep	given,was
R1066	T4116	T4117	det	the,importance
R1067	T4117	T4115	pobj	importance,given
R1068	T4118	T4117	prep	of,importance
R1069	T4119	T4120	det	the,gene
R107	T1059	T1060	compound	lens,agenesis
R1070	T4120	T4118	pobj	gene,of
R1071	T4121	T4120	compound	pygopus,gene
R1072	T4122	T4117	prep	in,importance
R1073	T4123	T4124	compound	Wnt,signaling
R1074	T4124	T4122	pobj	signaling,in
R1075	T4125	T4117	prep	in,importance
R1076	T4126	T4125	pobj	Drosophila,in
R1077	T4127	T4117	punct	", ",importance
R1078	T4128	T4117	cc	and,importance
R1079	T4129	T4130	det	the,expression
R108	T1060	T1058	pobj	agenesis,including
R1080	T4130	T4117	conj	expression,importance
R1081	T4131	T4130	amod	reported,expression
R1082	T4132	T4130	prep	during,expression
R1083	T4133	T4132	pobj	development,during
R1084	T4134	T4133	prep	of,development
R1085	T4135	T4136	det	the,gene
R1086	T4136	T4134	pobj	gene,of
R1087	T4137	T4136	compound	mouse,gene
R1088	T4138	T4136	compound	Pygo1,gene
R1089	T4139	T4130	prep	in,expression
R109	T1061	T1060	punct	", ",agenesis
R1090	T4140	T4139	punct	", ",in
R1091	T4141	T4139	prep	for,in
R1092	T4142	T4141	pobj	example,for
R1093	T4143	T4139	punct	", ",in
R1094	T4144	T4145	det	the,brain
R1095	T4145	T4139	pobj	brain,in
R1096	T4146	T4145	punct	", ",brain
R1097	T4147	T4145	conj	limbs,brain
R1098	T4148	T4147	punct	", ",limbs
R1099	T4149	T4147	conj	kidney,limbs
R11	T950	T951	amod	mammalian,kidney
R110	T1062	T1063	compound	growth,retardation
R1100	T4150	T4149	cc	and,kidney
R1101	T4151	T4152	amod	branchial,arches
R1102	T4152	T4149	conj	arches,kidney
R1103	T4153	T4154	punct	[,16
R1104	T4154	T4145	parataxis	16,brain
R1105	T4155	T4154	nummod	14,16
R1106	T4156	T4154	punct	",",16
R1107	T4157	T4154	punct	],16
R1108	T4158	T4159	punct	(,Yu
R1109	T4159	T4113	meta	Yu,was
R111	T1063	T1060	conj	retardation,agenesis
R1110	T4160	T4159	nmod	J,Yu
R1111	T4161	T4159	punct	", ",Yu
R1112	T4162	T4159	nmod	Valerius,Yu
R1113	T4163	T4159	nmod	MT,Yu
R1114	T4164	T4159	punct	", ",Yu
R1115	T4165	T4159	nmod	McMahon,Yu
R1116	T4166	T4159	nmod	AP,Yu
R1117	T4167	T4159	punct	", ",Yu
R1118	T4168	T4159	nmod	contribution,Yu
R1119	T4169	T4159	nmod	to,Yu
R112	T1064	T1063	punct	", ",retardation
R1120	T4170	T4159	nmod	GUDMAP,Yu
R1121	T4171	T4159	punct	", ",Yu
R1122	T4172	T4159	punct	),Yu
R1123	T4173	T4113	punct	.,was
R1124	T4175	T4176	det	The,mice
R1125	T4176	T4180	nsubj	mice,survived
R1126	T4177	T4178	npadvmod	Pygo2,null
R1127	T4178	T4176	amod	null,mice
R1128	T4179	T4178	amod	homozygous,null
R1129	T4181	T4180	prep	to,survived
R113	T1065	T1066	amod	altered,development
R1130	T4182	T4181	pobj	birth,to
R1131	T4183	T4180	punct	", ",survived
R1132	T4184	T4180	cc	but,survived
R1133	T4185	T4186	prep	with,died
R1134	T4186	T4180	conj	died,survived
R1135	T4187	T4188	amod	rare,exceptions
R1136	T4188	T4185	pobj	exceptions,with
R1137	T4189	T4186	punct	", ",died
R1138	T4190	T4191	advmod	shortly,afterwards
R1139	T4191	T4186	advmod	afterwards,died
R114	T1066	T1063	conj	development,retardation
R1140	T4192	T4180	punct	.,survived
R1141	T4194	T4195	det	The,gut
R1142	T4195	T4196	nsubj	gut,developed
R1143	T4197	T4195	punct	", ",gut
R1144	T4198	T4195	conj	heart,gut
R1145	T4199	T4198	cc	and,heart
R1146	T4200	T4198	conj	limbs,heart
R1147	T4201	T4196	prep	without,developed
R1148	T4202	T4203	amod	detectable,abnormality
R1149	T4203	T4201	pobj	abnormality,without
R115	T1067	T1066	compound	kidney,development
R1150	T4204	T4205	punct	(,shown
R1151	T4205	T4203	parataxis	shown,abnormality
R1152	T4206	T4205	nsubj	data,shown
R1153	T4207	T4205	neg	not,shown
R1154	T4208	T4205	punct	),shown
R1155	T4209	T4196	prep	despite,developed
R1156	T4210	T4211	amod	known,requirements
R1157	T4211	T4209	pobj	requirements,despite
R1158	T4212	T4211	prep	for,requirements
R1159	T4213	T4214	compound	Wnt,signaling
R116	T1068	T1066	punct	", ",development
R1160	T4214	T4212	pobj	signaling,for
R1161	T4215	T4196	punct	.,developed
R1162	T4217	T4218	det	The,mutants
R1163	T4218	T4220	nsubj	mutants,show
R1164	T4219	T4218	compound	Pygo2,mutants
R1165	T4221	T4220	aux	did,show
R1166	T4222	T4220	punct	", ",show
R1167	T4223	T4220	advmod	however,show
R1168	T4224	T4220	punct	", ",show
R1169	T4225	T4226	compound	growth,retardation
R117	T1069	T1066	cc	and,development
R1170	T4226	T4220	dobj	retardation,show
R1171	T4227	T4226	punct	", ",retardation
R1172	T4228	T4229	compound	lens,agenesis
R1173	T4229	T4226	conj	agenesis,retardation
R1174	T4230	T4229	cc	and,agenesis
R1175	T4231	T4232	det	a,phenotype
R1176	T4232	T4229	conj	phenotype,agenesis
R1177	T4233	T4232	compound	kidney,phenotype
R1178	T4234	T4232	prep	with,phenotype
R1179	T4235	T4236	amod	high,penetrance
R118	T1070	T1071	prep	in,exencephaly
R1180	T4236	T4234	pobj	penetrance,with
R1181	T4237	T4232	punct	", ",phenotype
R1182	T4238	T4232	conj	exencephaly,phenotype
R1183	T4239	T4238	punct	", ",exencephaly
R1184	T4240	T4238	cc	and,exencephaly
R1185	T4241	T4242	amod	cleft,palate
R1186	T4242	T4238	conj	palate,exencephaly
R1187	T4243	T4242	prep	with,palate
R1188	T4244	T4245	amod	incomplete,penetrance
R1189	T4245	T4243	pobj	penetrance,with
R119	T1071	T1066	conj	exencephaly,development
R1190	T4246	T4220	punct	.,show
R1191	T4248	T4249	amod	Double,homozygous
R1192	T4249	T4251	amod	homozygous,mice
R1193	T4250	T4249	punct	-,homozygous
R1194	T4251	T4256	nsubj	mice,had
R1195	T4252	T4251	nmod	mutant,mice
R1196	T4253	T4251	nmod	Pygo1,mice
R1197	T4254	T4253	cc	and,Pygo1
R1198	T4255	T4253	conj	Pygo2,Pygo1
R1199	T4257	T4258	det	a,phenotype
R12	T951	T952	compound	kidney,development
R120	T1072	T1073	det	some,cases
R1200	T4258	T4256	dobj	phenotype,had
R1201	T4259	T4258	amod	similar,phenotype
R1202	T4260	T4259	prep	to,similar
R1203	T4261	T4260	pobj	that,to
R1204	T4262	T4261	prep	of,that
R1205	T4263	T4264	amod	single,nulls
R1206	T4264	T4262	pobj	nulls,of
R1207	T4265	T4264	compound	Pygo2,nulls
R1208	T4266	T4267	punct	(,Table
R1209	T4267	T4256	parataxis	Table,had
R121	T1073	T1070	pobj	cases,in
R1210	T4268	T4267	dep	Figure,Table
R1211	T4269	T4268	nummod	1,Figure
R1212	T4270	T4267	punct	", ",Table
R1213	T4271	T4267	nummod	1,Table
R1214	T4272	T4267	punct	),Table
R1215	T4273	T4256	punct	.,had
R1216	T4275	T4276	expl	There,was
R1217	T4277	T4278	det	no,synergism
R1218	T4278	T4276	attr	synergism,was
R1219	T4279	T4278	amod	significant,synergism
R122	T1074	T1071	cc	and,exencephaly
R1220	T4280	T4278	prep	of,synergism
R1221	T4281	T4282	amod	developmental,abnormalities
R1222	T4282	T4280	pobj	abnormalities,of
R1223	T4283	T4278	prep	in,synergism
R1224	T4284	T4285	det	the,mutants
R1225	T4285	T4283	pobj	mutants,in
R1226	T4286	T4285	amod	double,mutants
R1227	T4287	T4276	punct	.,was
R1228	T4289	T4290	advmod	Together,suggest
R1229	T4291	T4292	det	these,results
R123	T1075	T1076	amod	cleft,palate
R1230	T4292	T4290	nsubj	results,suggest
R1231	T4293	T4294	mark	that,required
R1232	T4294	T4290	ccomp	required,suggest
R1233	T4295	T4294	nsubjpass	Pygo2,required
R1234	T4296	T4294	auxpass	is,required
R1235	T4297	T4294	prep	for,required
R1236	T4298	T4299	det	the,development
R1237	T4299	T4297	pobj	development,for
R1238	T4300	T4299	amod	proper,development
R1239	T4301	T4299	prep	of,development
R124	T1076	T1071	conj	palate,exencephaly
R1240	T4302	T4303	det	a,number
R1241	T4303	T4301	pobj	number,of
R1242	T4304	T4303	amod	limited,number
R1243	T4305	T4303	prep	of,number
R1244	T4306	T4305	pobj	structures,of
R1245	T4307	T4294	punct	", ",required
R1246	T4308	T4309	mark	whereas,is
R1247	T4309	T4294	advcl	is,required
R1248	T4310	T4309	nsubj	Pygo1,is
R1249	T4311	T4309	neg	not,is
R125	T1077	T1045	punct	.,died
R1250	T4312	T4309	acomp	necessary,is
R1251	T4313	T4312	prep	for,necessary
R1252	T4314	T4315	amod	normal,development
R1253	T4315	T4313	pobj	development,for
R1254	T4316	T4290	punct	.,suggest
R1257	T4630	T4629	prep	of,Expression
R1258	T4631	T4630	pobj	Pygo1,of
R1259	T4632	T4631	cc	and,Pygo1
R126	T1079	T1080	npadvmod	Pygo1,homozygous
R1260	T4633	T4631	conj	Pygo2,Pygo1
R1261	T4634	T4629	prep	in,Expression
R1262	T4635	T4636	det	the,kidney
R1263	T4636	T4634	pobj	kidney,in
R1264	T4637	T4636	amod	developing,kidney
R1265	T4639	T4640	advmod	In,situ
R1266	T4640	T4641	amod	situ,hybridization
R1267	T4641	T4642	nsubjpass	hybridization,used
R1268	T4643	T4642	aux	has,used
R1269	T4644	T4642	auxpass	been,used
R127	T1080	T1081	amod	homozygous,mutants
R1270	T4645	T4642	advmod	previously,used
R1271	T4646	T4647	aux	to,characterize
R1272	T4647	T4642	advcl	characterize,used
R1273	T4648	T4647	dobj	expression,characterize
R1274	T4649	T4648	prep	of,expression
R1275	T4650	T4649	pobj	Pygo1,of
R1276	T4651	T4652	punct	[,16
R1277	T4652	T4642	parataxis	16,used
R1278	T4653	T4652	nummod	14,16
R1279	T4654	T4652	punct	",",16
R128	T1081	T1082	nsubj	mutants,were
R1280	T4655	T4652	punct	],16
R1281	T4656	T4657	punct	(,Yu
R1282	T4657	T4642	meta	Yu,used
R1283	T4658	T4657	nmod	J,Yu
R1284	T4659	T4657	punct	", ",Yu
R1285	T4660	T4657	nmod	Valerius,Yu
R1286	T4661	T4657	nmod	MT,Yu
R1287	T4662	T4657	punct	", ",Yu
R1288	T4663	T4657	nmod	McMahon,Yu
R1289	T4664	T4657	nmod	AP,Yu
R129	T1083	T1082	punct	", ",were
R1290	T4665	T4657	punct	", ",Yu
R1291	T4666	T4657	nmod	contribution,Yu
R1292	T4667	T4657	nmod	to,Yu
R1293	T4668	T4657	nmod	GUDMAP,Yu
R1294	T4669	T4657	punct	", ",Yu
R1295	T4670	T4657	punct	),Yu
R1296	T4671	T4642	punct	.,used
R1297	T4673	T4674	prep	In,used
R1298	T4675	T4676	det	this,report
R1299	T4676	T4673	pobj	report,In
R13	T952	T949	pobj	development,in
R130	T1084	T1082	advmod	however,were
R1300	T4677	T4674	punct	", ",used
R1301	T4678	T4674	nsubj	we,used
R1302	T4679	T4674	dobj	immunofluorescence,used
R1303	T4680	T4681	aux	to,define
R1304	T4681	T4674	advcl	define,used
R1305	T4682	T4681	advmod	better,define
R1306	T4683	T4684	det	the,patterns
R1307	T4684	T4681	dobj	patterns,define
R1308	T4685	T4684	compound	expression,patterns
R1309	T4686	T4684	prep	of,patterns
R131	T1085	T1082	punct	", ",were
R1310	T4687	T4688	det	the,genes
R1311	T4688	T4686	pobj	genes,of
R1312	T4689	T4688	nmod	Pygo1,genes
R1313	T4690	T4689	cc	and,Pygo1
R1314	T4691	T4689	conj	Pygo2,Pygo1
R1315	T4692	T4684	prep	in,patterns
R1316	T4693	T4694	det	the,kidney
R1317	T4694	T4692	pobj	kidney,in
R1318	T4695	T4694	amod	developing,kidney
R1319	T4696	T4674	punct	.,used
R132	T1086	T1082	acomp	viable,were
R1320	T4698	T4699	det	Both,genes
R1321	T4699	T4700	nsubjpass	genes,expressed
R1322	T4701	T4700	auxpass	were,expressed
R1323	T4702	T4700	advmod	widely,expressed
R1324	T4703	T4700	punct	", ",expressed
R1325	T4704	T4700	advcl	showing,expressed
R1326	T4705	T4706	amod	nuclear,localization
R1327	T4706	T4704	dobj	localization,showing
R1328	T4707	T4706	prep	of,localization
R1329	T4708	T4709	amod	encoded,proteins
R133	T1087	T1086	cc	and,viable
R1330	T4709	T4707	pobj	proteins,of
R1331	T4710	T4706	prep	in,localization
R1332	T4711	T4712	det	the,bud
R1333	T4712	T4710	pobj	bud,in
R1334	T4713	T4712	amod	ureteric,bud
R1335	T4714	T4712	punct	", ",bud
R1336	T4715	T4716	compound	capping,mesenchyme
R1337	T4716	T4712	conj	mesenchyme,bud
R1338	T4717	T4716	punct	", ",mesenchyme
R1339	T4718	T4716	cc	and,mesenchyme
R134	T1088	T1086	conj	fertile,viable
R1340	T4719	T4720	amod	stromal,cells
R1341	T4720	T4716	conj	cells,mesenchyme
R1342	T4721	T4722	punct	(,Figure
R1343	T4722	T4700	parataxis	Figure,expressed
R1344	T4723	T4722	nummod	2,Figure
R1345	T4724	T4722	punct	),Figure
R1346	T4725	T4700	punct	.,expressed
R1347	T4727	T4728	amod	Raised,expression
R1348	T4728	T4729	nsubjpass	expression,seen
R1349	T4730	T4728	prep	of,expression
R135	T1089	T1082	punct	", ",were
R1350	T4731	T4730	pobj	Pygo1,of
R1351	T4732	T4730	punct	", ",of
R1352	T4733	T4730	cc	and,of
R1353	T4734	T4735	prep	to,of
R1354	T4735	T4730	conj	of,of
R1355	T4736	T4737	det	a,degree
R1356	T4737	T4734	pobj	degree,to
R1357	T4738	T4737	amod	lesser,degree
R1358	T4739	T4735	pobj	Pygo2,of
R1359	T4740	T4729	punct	", ",seen
R136	T1090	T1082	prep	with,were
R1360	T4741	T4729	auxpass	was,seen
R1361	T4742	T4729	prep	in,seen
R1362	T4743	T4744	amod	stromal,cells
R1363	T4744	T4742	pobj	cells,in
R1364	T4745	T4729	punct	", ",seen
R1365	T4746	T4729	cc	and,seen
R1366	T4747	T4748	amod	significant,staining
R1367	T4748	T4750	nsubjpass	staining,detected
R1368	T4749	T4748	amod	nuclear,staining
R1369	T4750	T4729	conj	detected,seen
R137	T1091	T1092	det	no,defects
R1370	T4751	T4750	auxpass	was,detected
R1371	T4752	T4750	prep	in,detected
R1372	T4753	T4754	advmod	essentially,cells
R1373	T4754	T4752	pobj	cells,in
R1374	T4755	T4754	det	all,cells
R1375	T4756	T4754	prep	of,cells
R1376	T4757	T4758	det	the,kidney
R1377	T4758	T4756	pobj	kidney,of
R1378	T4759	T4758	amod	developing,kidney
R1379	T4760	T4750	prep	for,detected
R138	T1092	T1090	pobj	defects,with
R1380	T4761	T4762	det	both,proteins
R1381	T4762	T4760	pobj	proteins,for
R1382	T4763	T4750	punct	.,detected
R1387	T5653	T5654	amod	Confocal,analysis
R1388	T5655	T5654	prep	of,analysis
R1389	T5656	T5657	compound	Pygo1,Pygo2
R139	T1093	T1092	amod	detectable,defects
R1390	T5657	T5659	compound	Pygo2,mutant
R1391	T5658	T5657	punct	/,Pygo2
R1392	T5659	T5660	compound	mutant,kidneys
R1393	T5660	T5655	pobj	kidneys,of
R1394	T5662	T5663	amod	Histological,examination
R1395	T5663	T5664	nsubj	examination,reveal
R1396	T5665	T5663	prep	of,examination
R1397	T5666	T5667	npadvmod	Pygo2,null
R1398	T5667	T5668	amod	null,kidneys
R1399	T5668	T5665	pobj	kidneys,of
R14	T957	T958	det	The,gene
R140	T1094	T1092	amod	developmental,defects
R1400	T5669	T5667	cc	and,null
R1401	T5670	T5671	compound	Pygo1,Pygo2
R1402	T5671	T5673	npadvmod	Pygo2,null
R1403	T5672	T5671	punct	/,Pygo2
R1404	T5673	T5667	conj	null,null
R1405	T5674	T5673	amod	double,null
R1406	T5675	T5673	punct	-,null
R1407	T5676	T5668	compound	mutant,kidneys
R1408	T5677	T5664	aux	did,reveal
R1409	T5678	T5664	neg	not,reveal
R141	T1095	T1082	punct	.,were
R1410	T5679	T5680	det	any,abnormalities
R1411	T5680	T5664	dobj	abnormalities,reveal
R1412	T5681	T5680	prep	in,abnormalities
R1413	T5682	T5681	pobj	nephrogenesis,in
R1414	T5683	T5684	punct	(,shown
R1415	T5684	T5664	parataxis	shown,reveal
R1416	T5685	T5684	nsubj	data,shown
R1417	T5686	T5684	neg	not,shown
R1418	T5687	T5684	punct	),shown
R1419	T5688	T5664	punct	.,reveal
R142	T1097	T1098	amod	Double,Pygo2
R1420	T5690	T5691	amod	Confocal,analysis
R1421	T5691	T5692	nsubjpass	analysis,performed
R1422	T5693	T5692	auxpass	was,performed
R1423	T5694	T5692	advmod	therefore,performed
R1424	T5695	T5696	aux	to,characterize
R1425	T5696	T5692	advcl	characterize,performed
R1426	T5697	T5698	amod	renal,development
R1427	T5698	T5696	dobj	development,characterize
R1428	T5699	T5698	prep	in,development
R1429	T5700	T5701	nmod	E18.5,kidneys
R143	T1098	T1101	npadvmod	Pygo2,homozygous
R1430	T5701	T5699	pobj	kidneys,in
R1431	T5702	T5703	compound	Pygo1,Pygo2
R1432	T5703	T5705	npadvmod	Pygo2,null
R1433	T5704	T5703	punct	/,Pygo2
R1434	T5705	T5701	amod	null,kidneys
R1435	T5706	T5707	advmod	more,precisely
R1436	T5707	T5696	advmod	precisely,characterize
R1437	T5708	T5709	punct	(,Figure
R1438	T5709	T5696	parataxis	Figure,characterize
R1439	T5710	T5709	nummod	3,Figure
R144	T1099	T1098	compound	Pygo1,Pygo2
R1440	T5711	T5709	punct	),Figure
R1441	T5712	T5692	punct	.,performed
R1442	T5714	T5715	nmod	Wt1,antibodies
R1443	T5715	T5724	nsubj	antibodies,stain
R1444	T5716	T5717	punct	(,red
R1445	T5717	T5714	parataxis	red,Wt1
R1446	T5718	T5717	punct	),red
R1447	T5719	T5714	cc	and,Wt1
R1448	T5720	T5714	conj	Cited1,Wt1
R1449	T5721	T5722	punct	(,red
R145	T1100	T1098	punct	/,Pygo2
R1450	T5722	T5720	parataxis	red,Cited1
R1451	T5723	T5722	punct	),red
R1452	T5725	T5715	appos	both,antibodies
R1453	T5726	T5727	det	the,mesenchyme
R1454	T5727	T5724	dobj	mesenchyme,stain
R1455	T5728	T5727	amod	capping,mesenchyme
R1456	T5729	T5727	amod	metanephric,mesenchyme
R1457	T5730	T5724	prep	around,stain
R1458	T5731	T5732	det	the,tips
R1459	T5732	T5730	pobj	tips,around
R146	T1101	T1102	amod	homozygous,mutants
R1460	T5733	T5734	amod	ureteric,bud
R1461	T5734	T5732	compound	bud,tips
R1462	T5735	T5736	punct	[,17
R1463	T5736	T5724	parataxis	17,stain
R1464	T5737	T5736	punct	],17
R1465	T5738	T5724	punct	.,stain
R1466	T5740	T5741	compound	WT1,antibody
R1467	T5741	T5742	nsubj	antibody,stains
R1468	T5743	T5742	advmod	also,stains
R1469	T5744	T5745	amod	renal,vesicles
R147	T1102	T1103	nsubj	mutants,showed
R1470	T5745	T5742	dobj	vesicles,stains
R1471	T5746	T5745	cc	and,vesicles
R1472	T5747	T5748	amod	glomerular,anlage
R1473	T5748	T5745	conj	anlage,vesicles
R1474	T5749	T5742	punct	.,stains
R1475	T5751	T5752	nsubjpass	Antibodies,used
R1476	T5753	T5751	prep	to,Antibodies
R1477	T5754	T5753	pobj	Cdh1,to
R1478	T5755	T5754	punct	", ",Cdh1
R1479	T5756	T5757	advmod	also,known
R148	T1104	T1105	det	no,synergy
R1480	T5757	T5754	acl	known,Cdh1
R1481	T5758	T5757	prep	as,known
R1482	T5759	T5760	compound	E,cadherin
R1483	T5760	T5758	pobj	cadherin,as
R1484	T5761	T5760	punct	-,cadherin
R1485	T5762	T5763	punct	(,blue
R1486	T5763	T5757	parataxis	blue,known
R1487	T5764	T5763	punct	),blue
R1488	T5765	T5752	punct	", ",used
R1489	T5766	T5752	auxpass	were,used
R149	T1105	T1103	dobj	synergy,showed
R1490	T5767	T5768	aux	to,identify
R1491	T5768	T5752	advcl	identify,used
R1492	T5769	T5770	amod	epithelial,structures
R1493	T5770	T5768	dobj	structures,identify
R1494	T5771	T5770	punct	", ",structures
R1495	T5772	T5770	prep	including,structures
R1496	T5773	T5774	det	the,bud
R1497	T5774	T5772	pobj	bud,including
R1498	T5775	T5774	amod	branching,bud
R1499	T5776	T5774	amod	ureteric,bud
R15	T958	T960	nsubj	gene,encodes
R150	T1106	T1105	amod	apparent,synergy
R1500	T5777	T5774	cc	and,bud
R1501	T5778	T5779	amod	nascent,nephrons
R1502	T5779	T5774	conj	nephrons,bud
R1503	T5780	T5779	prep	of,nephrons
R1504	T5781	T5782	det	the,kidney
R1505	T5782	T5780	pobj	kidney,of
R1506	T5783	T5782	amod	developing,kidney
R1507	T5784	T5785	punct	[,18
R1508	T5785	T5752	parataxis	18,used
R1509	T5786	T5785	punct	],18
R151	T1107	T1105	prep	in,synergy
R1510	T5787	T5752	punct	.,used
R1511	T5789	T5790	nmod	Dolichos,biflorus
R1512	T5790	T5791	nmod	biflorus,lectin
R1513	T5791	T5795	nsubjpass	lectin,used
R1514	T5792	T5790	punct	(,biflorus
R1515	T5793	T5790	appos	DBA,biflorus
R1516	T5794	T5791	punct	),lectin
R1517	T5796	T5797	punct	[,16
R1518	T5797	T5791	parataxis	16,lectin
R1519	T5798	T5797	punct	],16
R152	T1108	T1109	compound	phenotype,severity
R1520	T5799	T5795	auxpass	was,used
R1521	T5800	T5801	aux	to,stain
R1522	T5801	T5795	advcl	stain,used
R1523	T5802	T5801	advmod	selectively,stain
R1524	T5803	T5804	amod	ureteric,bud
R1525	T5804	T5805	npadvmod	bud,derived
R1526	T5805	T5807	amod	derived,structures
R1527	T5806	T5805	punct	-,derived
R1528	T5807	T5801	dobj	structures,stain
R1529	T5808	T5809	punct	[,19
R153	T1109	T1107	pobj	severity,in
R1530	T5809	T5801	parataxis	19,stain
R1531	T5810	T5809	punct	],19
R1532	T5811	T5795	punct	.,used
R1533	T5820	T5818	cc	d,C
R1534	T5833	T5832	pobj	n,i
R1535	T5839	T5840	det	an,increase
R1536	T5840	T5838	dobj	increase,vealed
R1537	T5841	T5840	prep	of,increase
R1538	T5842	T5843	advmod	approximately,30
R1539	T5843	T5844	nummod	30,%
R154	T1110	T1103	punct	.,showed
R1540	T5844	T5841	pobj	%,of
R1541	T5845	T5840	prep	in,increase
R1542	T5846	T5847	det	the,thickness
R1543	T5847	T5845	pobj	thickness,in
R1544	T5848	T5847	prep	of,thickness
R1545	T5849	T5850	det	the,mesenchyme
R1546	T5850	T5848	pobj	mesenchyme,of
R1547	T5851	T5850	amod	capping,mesenchyme
R1548	T5852	T5850	acl	surrounding,mesenchyme
R1549	T5853	T5854	det	the,buds
R155	T1112	T1113	det	The,reporter
R1550	T5854	T5852	dobj	buds,surrounding
R1551	T5855	T5854	nmod	mutant,buds
R1552	T5856	T5854	amod	ureteric,buds
R1553	T5857	T5858	punct	(,Table
R1554	T5858	T5838	parataxis	Table,vealed
R1555	T5859	T5858	dep	Figure,Table
R1556	T5860	T5859	nummod	3,Figure
R1557	T5861	T5858	punct	", ",Table
R1558	T5862	T5858	nummod	2,Table
R1559	T5863	T5858	punct	),Table
R156	T1113	T1118	nsubj	reporter,showed
R1560	T5864	T5838	punct	.,vealed
R1561	T5866	T5867	advmod	Nevertheless,underwent
R1562	T5868	T5867	punct	", ",underwent
R1563	T5869	T5870	det	the,mesenchyme
R1564	T5870	T5867	nsubj	mesenchyme,underwent
R1565	T5871	T5870	nmod	mutant,mesenchyme
R1566	T5872	T5870	amod	metanephric,mesenchyme
R1567	T5873	T5874	advmod	relatively,normal
R1568	T5874	T5875	amod	normal,nephrogenesis
R1569	T5875	T5867	dobj	nephrogenesis,underwent
R157	T1114	T1115	compound	BAT,gal
R1570	T5876	T5867	punct	.,underwent
R1571	T5878	T5879	npadvmod	Cdh1,staining
R1572	T5879	T5881	amod	staining,nephrons
R1573	T5880	T5879	punct	-,staining
R1574	T5881	T5882	nsubjpass	nephrons,identified
R1575	T5883	T5884	punct	(,blue
R1576	T5884	T5881	parataxis	blue,nephrons
R1577	T5885	T5884	punct	),blue
R1578	T5886	T5882	auxpass	were,identified
R1579	T5887	T5882	advcl	connecting,identified
R158	T1115	T1117	compound	gal,transgene
R1580	T5888	T5887	prep	to,connecting
R1581	T5889	T5890	det	the,tips
R1582	T5890	T5888	pobj	tips,to
R1583	T5891	T5892	amod	ureteric,bud
R1584	T5892	T5890	compound	bud,tips
R1585	T5893	T5894	punct	[,16
R1586	T5894	T5887	parataxis	16,connecting
R1587	T5895	T5894	punct	],16
R1588	T5896	T5887	cc	and,connecting
R1589	T5897	T5887	conj	extending,connecting
R159	T1116	T1115	punct	-,gal
R1590	T5898	T5897	prep	into,extending
R1591	T5899	T5900	det	the,medulla
R1592	T5900	T5898	pobj	medulla,into
R1593	T5901	T5900	prep	of,medulla
R1594	T5902	T5903	det	the,kidney
R1595	T5903	T5901	pobj	kidney,of
R1596	T5904	T5897	punct	", ",extending
R1597	T5905	T5906	mark	as,normal
R1598	T5906	T5897	advcl	normal,extending
R1599	T5907	T5882	punct	.,identified
R16	T959	T958	compound	pygopus,gene
R160	T1117	T1113	compound	transgene,reporter
R1600	T5909	T5910	amod	Intermediate,structures
R1601	T5910	T5911	nsubj	structures,appeared
R1602	T5912	T5910	prep	of,structures
R1603	T5913	T5912	pobj	nephrogenesis,of
R1604	T5914	T5910	punct	", ",structures
R1605	T5915	T5910	prep	including,structures
R1606	T5916	T5917	amod	renal,vesicles
R1607	T5917	T5915	pobj	vesicles,including
R1608	T5918	T5917	punct	", ",vesicles
R1609	T5919	T5920	npadvmod	comma,shaped
R161	T1119	T1113	prep	of,reporter
R1610	T5920	T5922	amod	shaped,bodies
R1611	T5921	T5920	punct	-,shaped
R1612	T5922	T5917	conj	bodies,vesicles
R1613	T5923	T5922	punct	", ",bodies
R1614	T5924	T5922	cc	and,bodies
R1615	T5925	T5926	npadvmod	S,shaped
R1616	T5926	T5928	amod	shaped,bodies
R1617	T5927	T5926	punct	-,shaped
R1618	T5928	T5922	conj	bodies,bodies
R1619	T5929	T5911	oprd	normal,appeared
R162	T1120	T1121	amod	canonical,signaling
R1620	T5930	T5911	punct	.,appeared
R1621	T5933	T5934	amod	Confocal,analysis
R1622	T5938	T5934	prep	o,analysis
R1623	T5943	T5944	mark	that,were
R1624	T5944	T5942	ccomp	were,howed
R1625	T5945	T5946	det	the,tips
R1626	T5946	T5944	nsubj	tips,were
R1627	T5947	T5948	amod	ureteric,bud
R1628	T5948	T5946	compound	bud,tips
R1629	T5949	T5950	punct	[,16
R163	T1121	T1119	pobj	signaling,of
R1630	T5950	T5946	parataxis	16,tips
R1631	T5951	T5950	punct	],16
R1632	T5952	T5946	prep	of,tips
R1633	T5953	T5954	compound	Pygo1,Pygo2
R1634	T5954	T5956	npadvmod	Pygo2,null
R1635	T5955	T5954	punct	/,Pygo2
R1636	T5956	T5957	amod	null,kidneys
R1637	T5957	T5952	pobj	kidneys,of
R1638	T5958	T5944	acomp	dilated,were
R1639	T5959	T5958	cc	and,dilated
R164	T1122	T1121	compound	Wnt,signaling
R1640	T5960	T5958	conj	misshaped,dilated
R1641	T5961	T5944	prep	compared,were
R1642	T5962	T5961	prep	with,compared
R1643	T5963	T5962	pobj	those,with
R1644	T5964	T5963	prep	of,those
R1645	T5965	T5964	pobj	littermates,of
R1646	T5966	T5965	prep	with,littermates
R1647	T5967	T5968	advmod	at,one
R1648	T5968	T5970	nummod	one,allele
R1649	T5969	T5968	advmod	least,one
R165	T1123	T1124	amod	reduced,levels
R1650	T5970	T5966	pobj	allele,with
R1651	T5971	T5972	amod	wild,type
R1652	T5972	T5970	compound	type,allele
R1653	T5973	T5972	punct	-,type
R1654	T5974	T5970	compound	Pygo2,allele
R1655	T5975	T5976	punct	(,Figure
R1656	T5976	T5944	parataxis	Figure,were
R1657	T5977	T5976	nummod	3,Figure
R1658	T5978	T5976	punct	),Figure
R1659	T5979	T5942	punct	.,howed
R166	T1124	T1118	dobj	levels,showed
R1660	T5981	T5982	prep	In,had
R1661	T5983	T5981	pobj	addition,In
R1662	T5984	T5982	punct	", ",had
R1663	T5985	T5986	det	the,kidneys
R1664	T5986	T5982	nsubj	kidneys,had
R1665	T5987	T5988	compound	Pygo1,Pygo2
R1666	T5988	T5990	npadvmod	Pygo2,homozygous
R1667	T5989	T5988	punct	/,Pygo2
R1668	T5990	T5986	amod	homozygous,kidneys
R1669	T5991	T5990	amod	double,homozygous
R167	T1125	T1124	prep	of,levels
R1670	T5992	T5990	punct	-,homozygous
R1671	T5993	T5986	compound	mutant,kidneys
R1672	T5994	T5982	advmod	also,had
R1673	T5995	T5996	det	a,decrease
R1674	T5996	T5982	dobj	decrease,had
R1675	T5997	T5996	prep	of,decrease
R1676	T5998	T5999	advmod	approximately,25
R1677	T5999	T6000	nummod	25,%
R1678	T6000	T5997	pobj	%,of
R1679	T6001	T5996	prep	in,decrease
R168	T1126	T1125	pobj	expression,of
R1680	T6002	T6003	det	the,number
R1681	T6003	T6001	pobj	number,in
R1682	T6004	T6003	prep	of,number
R1683	T6005	T6006	amod	ureteric,bud
R1684	T6006	T6007	compound	bud,tips
R1685	T6007	T6004	pobj	tips,of
R1686	T6008	T6007	prep	per,tips
R1687	T6009	T6008	pobj	area,per
R1688	T6010	T6003	prep	compared,number
R1689	T6011	T6010	prep	with,compared
R169	T1127	T1124	prep	in,levels
R1690	T6012	T6011	pobj	littermates,with
R1691	T6013	T6012	prep	with,littermates
R1692	T6014	T6015	advmod	at,one
R1693	T6015	T6017	nummod	one,copy
R1694	T6016	T6015	advmod	least,one
R1695	T6017	T6013	pobj	copy,with
R1696	T6018	T6019	amod	wild,type
R1697	T6019	T6017	compound	type,copy
R1698	T6020	T6019	punct	-,type
R1699	T6021	T6017	prep	of,copy
R17	T961	T958	prep	of,gene
R170	T1128	T1129	nmod	Pygo1,mutants
R1700	T6022	T6021	pobj	Pygo2,of
R1701	T6023	T6024	punct	(,Table
R1702	T6024	T5996	parataxis	Table,decrease
R1703	T6025	T6024	nummod	2,Table
R1704	T6026	T6024	punct	),Table
R1705	T6027	T5982	punct	.,had
R1706	T6029	T6030	det	No,difference
R1707	T6030	T6032	nsubjpass	difference,seen
R1708	T6031	T6030	amod	significant,difference
R1709	T6033	T6030	prep	in,difference
R171	T1129	T1127	pobj	mutants,in
R1710	T6034	T6035	amod	ureteric,bud
R1711	T6035	T6036	compound	bud,tip
R1712	T6036	T6038	compound	tip,density
R1713	T6037	T6036	punct	-,tip
R1714	T6038	T6033	pobj	density,in
R1715	T6039	T6032	auxpass	was,seen
R1716	T6040	T6032	prep	between,seen
R1717	T6041	T6042	nmod	Pygo2,embryos
R1718	T6042	T6040	pobj	embryos,between
R1719	T6043	T6041	punct	+,Pygo2
R172	T1130	T1128	punct	-,Pygo1
R1720	T6044	T6041	punct	/,Pygo2
R1721	T6045	T6041	punct	-,Pygo2
R1722	T6046	T6041	cc	and,Pygo2
R1723	T6047	T6041	conj	Pygo2,Pygo2
R1724	T6048	T6047	punct	+,Pygo2
R1725	T6049	T6047	punct	/,Pygo2
R1726	T6050	T6047	punct	+,Pygo2
R1727	T6051	T6052	punct	(,0.33
R1728	T6052	T6032	parataxis	0.33,seen
R1729	T6053	T6052	nsubj	P,0.33
R173	T1131	T1128	punct	/,Pygo1
R1730	T6054	T6052	punct	=,0.33
R1731	T6055	T6052	punct	),0.33
R1732	T6056	T6032	punct	.,seen
R1739	T6317	T6318	advmod	In,situ
R174	T1132	T1128	punct	-,Pygo1
R1740	T6318	T6319	amod	situ,hybridization
R1741	T6321	T6322	nsubj	We,examined
R1742	T6323	T6322	dobj	expression,examined
R1743	T6324	T6323	prep	of,expression
R1744	T6325	T6326	nummod	three,genes
R1745	T6326	T6324	pobj	genes,of
R1746	T6327	T6326	compound	Wnt,genes
R1747	T6328	T6323	prep	in,expression
R1748	T6329	T6330	compound	Pygo,mutants
R1749	T6330	T6328	pobj	mutants,in
R175	T1133	T1128	punct	/,Pygo1
R1750	T6331	T6322	punct	.,examined
R1751	T6333	T6334	nsubjpass	Wnt7b,expressed
R1752	T6335	T6334	auxpass	is,expressed
R1753	T6336	T6334	prep	in,expressed
R1754	T6337	T6338	det	the,stalks
R1755	T6338	T6336	pobj	stalks,in
R1756	T6339	T6340	punct	[,20
R1757	T6340	T6334	parataxis	20,expressed
R1758	T6341	T6340	punct	],20
R1759	T6342	T6334	punct	", ",expressed
R176	T1134	T1128	appos	Pygo2,Pygo1
R1760	T6343	T6334	conj	Wnt11,expressed
R1761	T6344	T6343	prep	in,Wnt11
R1762	T6345	T6346	det	the,tips
R1763	T6346	T6344	pobj	tips,in
R1764	T6347	T6348	punct	[,4
R1765	T6348	T6343	parataxis	4,Wnt11
R1766	T6349	T6348	punct	],4
R1767	T6350	T6343	punct	", ",Wnt11
R1768	T6351	T6343	cc	and,Wnt11
R1769	T6352	T6343	conj	Wnt9b,Wnt11
R177	T1135	T1134	punct	-,Pygo2
R1770	T6353	T6352	prep	in,Wnt9b
R1771	T6354	T6355	det	the,stalks
R1772	T6355	T6353	pobj	stalks,in
R1773	T6356	T6353	cc	and,in
R1774	T6357	T6358	advmod	weakly,in
R1775	T6358	T6353	conj	in,in
R1776	T6359	T6360	det	the,tips
R1777	T6360	T6358	pobj	tips,in
R1778	T6361	T6360	prep	of,tips
R1779	T6362	T6363	det	the,bud
R178	T1136	T1134	punct	/,Pygo2
R1780	T6363	T6361	pobj	bud,of
R1781	T6364	T6363	nmod	branching,bud
R1782	T6365	T6363	amod	ureteric,bud
R1783	T6366	T6367	punct	[,2
R1784	T6367	T6352	parataxis	2,Wnt9b
R1785	T6368	T6367	punct	],2
R1786	T6369	T6352	punct	.,Wnt9b
R1787	T6371	T6372	det	All,genes
R1788	T6372	T6374	nsubj	genes,showed
R1789	T6373	T6372	nummod	three,genes
R179	T1137	T1134	punct	-,Pygo2
R1790	T6375	T6376	amod	similar,patterns
R1791	T6376	T6374	dobj	patterns,showed
R1792	T6377	T6376	compound	expression,patterns
R1793	T6378	T6374	prep	in,showed
R1794	T6379	T6380	nmod	Pygo2,kidneys
R1795	T6380	T6378	pobj	kidneys,in
R1796	T6381	T6379	punct	+,Pygo2
R1797	T6382	T6379	punct	/,Pygo2
R1798	T6383	T6379	punct	+,Pygo2
R1799	T6384	T6379	cc	and,Pygo2
R18	T962	T961	pobj	Drosophila,of
R180	T1138	T1118	punct	", ",showed
R1800	T6385	T6386	nmod	Pygo1,Pygo2
R1801	T6386	T6388	nmod	Pygo2,mutant
R1802	T6387	T6386	punct	/,Pygo2
R1803	T6388	T6379	conj	mutant,Pygo2
R1804	T6389	T6388	amod	double,mutant
R1805	T6390	T6388	punct	-,mutant
R1806	T6391	T6392	punct	(,Figure
R1807	T6392	T6374	parataxis	Figure,showed
R1808	T6393	T6392	nummod	4,Figure
R1809	T6394	T6392	punct	),Figure
R181	T1139	T1118	prep	with,showed
R1810	T6395	T6374	punct	.,showed
R1811	T6397	T6398	prep	In,confirmed
R1812	T6399	T6397	pobj	addition,In
R1813	T6400	T6398	punct	", ",confirmed
R1814	T6401	T6402	det	these,patterns
R1815	T6402	T6398	nsubj	patterns,confirmed
R1816	T6403	T6404	advmod	in,situ
R1817	T6404	T6405	amod	situ,hybridization
R1818	T6405	T6402	compound	hybridization,patterns
R1819	T6406	T6407	det	the,results
R182	T1140	T1141	npadvmod	tissue,specific
R1820	T6407	T6398	dobj	results,confirmed
R1821	T6408	T6409	amod	confocal,microscopy
R1822	T6409	T6407	compound	microscopy,results
R1823	T6410	T6398	punct	", ",confirmed
R1824	T6411	T6398	advcl	showing,confirmed
R1825	T6412	T6413	det	a,number
R1826	T6413	T6411	dobj	number,showing
R1827	T6414	T6413	amod	reduced,number
R1828	T6415	T6413	prep	of,number
R1829	T6416	T6415	pobj	tips,of
R183	T1141	T1143	amod	specific,variation
R1830	T6417	T6416	punct	(,tips
R1831	T6418	T6419	npadvmod	Wnt11,positive
R1832	T6419	T6416	amod	positive,tips
R1833	T6420	T6419	punct	-,positive
R1834	T6421	T6416	punct	),tips
R1835	T6422	T6416	prep	per,tips
R1836	T6423	T6424	compound	surface,area
R1837	T6424	T6422	pobj	area,per
R1838	T6425	T6413	prep	in,number
R1839	T6426	T6427	det	the,mutants
R184	T1142	T1141	punct	-,specific
R1840	T6427	T6425	pobj	mutants,in
R1841	T6428	T6429	compound	Pygo1,Pygo2
R1842	T6429	T6431	npadvmod	Pygo2,homozygous
R1843	T6430	T6429	punct	/,Pygo2
R1844	T6431	T6427	amod	homozygous,mutants
R1845	T6432	T6431	amod	double,homozygous
R1846	T6433	T6431	punct	-,homozygous
R1847	T6434	T6398	punct	.,confirmed
R185	T1143	T1139	pobj	variation,with
R1858	T9319	T9320	amod	Reduced,signaling
R1859	T9321	T9320	amod	canonical,signaling
R186	T1144	T1143	prep	in,variation
R1860	T9322	T9320	compound	Wnt,signaling
R1861	T9323	T9320	prep	in,signaling
R1862	T9324	T9325	compound	Pygo1,Pygo2
R1863	T9325	T9327	compound	Pygo2,mutant
R1864	T9326	T9325	punct	/,Pygo2
R1865	T9327	T9328	compound	mutant,mice
R1866	T9328	T9323	pobj	mice,in
R1867	T9330	T9331	det	The,reporter
R1868	T9331	T9336	nsubjpass	reporter,used
R1869	T9332	T9333	compound	BAT,gal
R187	T1145	T1144	pobj	degree,in
R1870	T9333	T9335	compound	gal,transgene
R1871	T9334	T9333	punct	-,gal
R1872	T9335	T9331	compound	transgene,reporter
R1873	T9337	T9331	prep	of,reporter
R1875	T9338	T9339	amod	canonical,signaling
R1877	T9339	T9337	pobj	signaling,of
R1878	T9340	T9339	compound	Wnt,signaling
R1879	T9341	T9342	punct	[,21
R188	T1146	T1145	prep	of,degree
R1880	T9342	T9331	parataxis	21,reporter
R1881	T9343	T9342	punct	],21
R1882	T9344	T9336	auxpass	was,used
R1883	T9345	T9346	aux	to,examine
R1884	T9346	T9336	advcl	examine,used
R1885	T9347	T9346	dobj	changes,examine
R1886	T9348	T9347	prep	in,changes
R1887	T9349	T9350	compound	Wnt,signaling
R1888	T9350	T9348	pobj	signaling,in
R1889	T9351	T9347	prep	in,changes
R189	T1147	T1146	pobj	diminution,of
R1890	T9352	T9353	det	the,mice
R1891	T9353	T9351	pobj	mice,in
R1892	T9354	T9353	compound	mutant,mice
R1893	T9410	T9411	mark	while,showed
R1894	T9355	T9336	punct	.,used
R1895	T9411	T9402	advcl	showed,showing
R1896	T9412	T9413	det	the,telencephalon
R1897	T9413	T9411	nsubj	telencephalon,showed
R1898	T9414	T9413	compound	mutant,telencephalon
R1899	T9415	T9411	advmod	still,showed
R19	T963	T964	det	an,component
R190	T1148	T1118	punct	.,showed
R1900	T9416	T9417	amod	robust,signaling
R1901	T9417	T9411	dobj	signaling,showed
R1902	T9418	T9417	compound	Wnt,signaling
R1903	T9419	T9385	punct	.,was
R1904	T9421	T9422	det	These,results
R1905	T9422	T9423	nsubj	results,suggest
R1906	T9357	T9358	preconj	Both,Pygo2
R1907	T9424	T9425	mark	that,are
R1908	T9425	T9423	ccomp	are,suggest
R1909	T9426	T9427	det	the,genes
R191	T1150	T1151	det	The,genes
R1910	T9427	T9425	nsubj	genes,are
R1911	T9428	T9427	amod	mammalian,genes
R1912	T9358	T9359	npadvmod	Pygo2,null
R1913	T9429	T9427	compound	Pygo,genes
R1914	T9430	T9431	amod	significant,modulators
R1915	T9431	T9425	attr	modulators,are
R1916	T9432	T9431	prep	of,modulators
R1917	T9359	T9369	amod	null,embryos
R1918	T9433	T9434	amod	canonical,signaling
R1919	T9434	T9432	pobj	signaling,of
R192	T1151	T1155	nsubj	genes,showed
R1920	T9360	T9358	punct	-,Pygo2
R1921	T9435	T9434	compound	Wnt,signaling
R1922	T9436	T9425	prep	in,are
R1923	T9437	T9436	pobj	some,in
R1924	T9438	T9436	punct	", ",in
R1925	T9361	T9358	punct	/,Pygo2
R1926	T9439	T9436	cc	but,in
R1927	T9362	T9358	punct	-,Pygo2
R1928	T9440	T9439	neg	not,but
R1929	T9441	T9436	conj	in,in
R193	T1152	T1151	nmod	Pygo1,genes
R1930	T9442	T9441	pobj	all,in
R1931	T9443	T9444	amod	developing,systems
R1932	T9363	T9358	cc	and,Pygo2
R1933	T9444	T9441	pobj	systems,in
R1934	T9445	T9423	punct	.,suggest
R1935	T9364	T9365	compound	Pygo1,Pygo2
R1936	T9365	T9358	conj	Pygo2,Pygo2
R1937	T9366	T9365	punct	/,Pygo2
R1938	T9453	T9452	compound	s,o
R1939	T9367	T9359	amod	double,null
R194	T1153	T1152	cc	and,Pygo1
R1940	T9368	T9359	punct	-,null
R1941	T9369	T9371	nsubj	embryos,showed
R1942	T9370	T9369	compound	E10.5,embryos
R1943	T9372	T9373	det	a,decrease
R1944	T9373	T9371	dobj	decrease,showed
R1945	T9374	T9373	prep	in,decrease
R1946	T9375	T9376	amod	canonical,signaling
R1947	T9376	T9374	pobj	signaling,in
R1948	T9465	T9466	compound	BAT,gal
R1949	T9466	T9464	compound	gal,re
R195	T1154	T1152	conj	Pygo2,Pygo1
R1950	T9467	T9466	punct	-,gal
R1951	T9377	T9376	compound	Wnt,signaling
R1952	T9378	T9379	punct	(,Figure
R1953	T9379	T9371	parataxis	Figure,showed
R1954	T9380	T9379	nummod	5,Figure
R1955	T9381	T9379	punct	),Figure
R1956	T9382	T9371	punct	.,showed
R1957	T9384	T9385	expl	There,was
R1958	T9386	T9385	punct	", ",was
R1959	T9478	T9479	compound	pre,ssion
R196	T1156	T1151	appos	both,genes
R1960	T9479	T9477	dobj	ssion,x
R1961	T9387	T9385	advmod	however,was
R1962	T9480	T9477	prep	in,x
R1963	T9481	T9482	amod	more,detail
R1964	T9482	T9480	pobj	detail,in
R1965	T9388	T9385	punct	", ",was
R1966	T9483	T9477	prep	in,x
R1967	T9484	T9485	det	the,system
R1968	T9389	T9390	npadvmod	tissue,specific
R1969	T9390	T9392	amod	specific,variability
R197	T1157	T1158	amod	widespread,expression
R1970	T9485	T9483	pobj	system,in
R1971	T9391	T9390	punct	-,specific
R1972	T9486	T9485	amod	developing,system
R1973	T9487	T9485	amod	urogenital,system
R1974	T9392	T9385	attr	variability,was
R1975	T9488	T9485	prep	of,system
R1976	T9489	T9490	compound	Pygo,mutants
R1977	T9490	T9488	pobj	mutants,of
R1978	T9393	T9392	prep	in,variability
R1979	T9491	T9477	punct	.,x
R198	T1158	T1155	dobj	expression,showed
R1980	T9394	T9395	det	the,degree
R1981	T9493	T9494	det	The,results
R1982	T9395	T9393	pobj	degree,in
R1983	T9396	T9395	prep	of,degree
R1984	T9494	T9495	nsubj	results,suggested
R1985	T9397	T9396	pobj	reduction,of
R1986	T9496	T9497	mark	that,required
R1987	T9497	T9495	ccomp	required,suggested
R1988	T9398	T9385	punct	", ",was
R1989	T9498	T9499	det	the,gene
R199	T1159	T1158	prep	in,expression
R1990	T9499	T9497	nsubjpass	gene,required
R1991	T9500	T9499	compound	Pygo2,gene
R1992	T9399	T9385	prep	with,was
R1993	T9501	T9497	auxpass	is,required
R1994	T9502	T9497	prep	for,required
R1995	T9503	T9504	amod	canonical,signaling
R1996	T9400	T9401	det	the,somites
R1997	T9504	T9502	pobj	signaling,for
R1998	T9505	T9504	compound	Wnt,signaling
R1999	T9401	T9402	nsubj	somites,showing
R20	T964	T960	dobj	component,encodes
R200	T1160	T1161	det	the,kidney
R2000	T9506	T9504	prep	in,signaling
R2001	T9507	T9508	det	the,duct
R2002	T9508	T9506	pobj	duct,in
R2003	T9402	T9399	pcomp	showing,with
R2004	T9403	T9402	punct	", ",showing
R2005	T9509	T9508	amod	nephric,duct
R2006	T9510	T9495	punct	.,suggested
R2007	T9404	T9402	prep	for,showing
R2008	T9512	T9513	preconj	Both,null
R2009	T9513	T9515	amod	null,embryos
R201	T1161	T1159	pobj	kidney,in
R2010	T9405	T9404	pobj	example,for
R2011	T9514	T9513	npadvmod	Pygo2,null
R2012	T9515	T9524	nsubj	embryos,showed
R2013	T9516	T9513	cc	and,null
R2014	T9406	T9402	punct	", ",showing
R2015	T9517	T9518	compound	Pygo1,Pygo2
R2016	T9518	T9520	npadvmod	Pygo2,null
R2017	T9519	T9518	punct	/,Pygo2
R2018	T9407	T9408	amod	strong,reduction
R2019	T9520	T9513	conj	null,null
R202	T1162	T1161	amod	developing,kidney
R2020	T9521	T9520	amod	double,null
R2021	T9522	T9520	punct	-,null
R2022	T9408	T9402	dobj	reduction,showing
R2023	T9523	T9515	compound	E10.5,embryos
R2024	T9525	T9526	det	an,absence
R2025	T9409	T9402	punct	", ",showing
R2026	T9526	T9524	dobj	absence,showed
R2027	T9527	T9526	prep	of,absence
R2028	T9528	T9529	compound	reporter,expression
R2029	T9591	T9587	punct	),6A
R203	T1163	T1155	punct	", ",showed
R2030	T9529	T9527	pobj	expression,of
R2031	T9530	T9526	prep	in,absence
R2032	T9531	T9532	det	the,duct
R2033	T9592	T9550	punct	.,form
R2034	T9532	T9530	pobj	duct,in
R2035	T9533	T9532	amod	nephric,duct
R2036	T9534	T9524	punct	", ",showed
R2037	T9599	T9598	conj	3.5,E1
R2038	T9535	T9536	mark	while,showed
R2039	T9536	T9524	advcl	showed,showed
R204	T1164	T1155	prep	with,showed
R2040	T9537	T9538	compound	control,littermates
R2041	T9538	T9536	nsubj	littermates,showed
R2042	T9539	T9540	amod	strong,expression
R2043	T9540	T9536	dobj	expression,showed
R2044	T9541	T9542	punct	(,6A
R2045	T9542	T9524	parataxis	6A,showed
R2046	T9543	T9542	compound	Figure,6A
R2047	T9544	T9545	punct	–,C
R2048	T9545	T9542	prep	C,6A
R2049	T9546	T9542	punct	),6A
R205	T1165	T1166	amod	raised,levels
R2050	T9614	T9615	compound	p,pe
R2051	T9547	T9524	punct	.,showed
R2052	T9549	T9550	prep	In,form
R2053	T9551	T9552	compound	Pygo1,Pygo2
R2054	T9552	T9554	npadvmod	Pygo2,null
R2055	T9553	T9552	punct	/,Pygo2
R2056	T9554	T9557	amod	null,mutants
R2057	T9555	T9554	amod	double,null
R2058	T9556	T9554	punct	-,null
R2059	T9557	T9549	pobj	mutants,In
R206	T1166	T1164	pobj	levels,with
R2060	T9558	T9550	punct	", ",form
R2061	T9559	T9560	det	the,duct
R2062	T9560	T9550	nsubj	duct,form
R2063	T9561	T9560	amod	nephric,duct
R2064	T9562	T9550	aux	did,form
R2065	T9563	T9550	punct	", ",form
R2066	T9564	T9550	advmod	however,form
R2067	T9565	T9550	punct	", ",form
R2068	T9566	T9550	cc	and,form
R2069	T9567	T9550	conj	give,form
R207	T1167	T1166	prep	in,levels
R2070	T9568	T9567	dobj	rise,give
R2071	T9569	T9567	prep	to,give
R2072	T9570	T9571	det	the,outgrowth
R2073	T9571	T9569	pobj	outgrowth,to
R2074	T9572	T9573	amod	ureteric,bud
R2075	T9573	T9571	compound	bud,outgrowth
R2076	T9574	T9571	punct	", ",outgrowth
R2077	T9627	T9626	punct	,y
R2078	T9575	T9576	dep	which,showed
R2079	T9576	T9571	relcl	showed,outgrowth
R208	T1168	T1169	det	the,compartment
R2080	T9577	T9578	amod	reduced,expression
R2081	T9578	T9576	dobj	expression,showed
R2082	T9579	T9577	cc	but,reduced
R2083	T9580	T9579	neg	not,but
R2084	T9581	T9577	conj	absent,reduced
R2085	T9582	T9583	compound	BAT,gal
R2086	T9583	T9585	compound	gal,reporter
R2087	T9584	T9583	punct	-,gal
R2088	T9585	T9578	compound	reporter,expression
R2089	T9586	T9587	punct	(,6A
R209	T1169	T1167	pobj	compartment,in
R2090	T9587	T9576	parataxis	6A,showed
R2091	T9588	T9587	compound	Figure,6A
R2092	T9589	T9590	punct	–,C
R2093	T9632	T9631	amod	jor,role
R2094	T9590	T9587	prep	C,6A
R2095	T9635	T9636	det	the,gene
R2096	T9636	T9638	nsubj	gene,role
R2097	T9637	T9636	compound	Pygo1,gene
R2098	T9639	T9638	det	a,role
R2099	T9643	T9644	amod	canonical,signaling
R21	T965	T964	amod	essential,component
R210	T1170	T1171	amod	stromal,cell
R2100	T9640	T9638	amod	minor,role
R2101	T9644	T9642	pobj	signaling,in
R2102	T9645	T9644	compound	Wnt,signaling
R2103	T9641	T9638	punct	", ",role
R2104	T9646	T9644	prep	in,signaling
R2105	T9647	T9648	det	the,tree
R2106	T9648	T9646	pobj	tree,in
R2107	T9649	T9648	amod	ureteric,tree
R2108	T9651	T9652	mark	as,measured
R2109	T9642	T9638	conj	in,role
R211	T1171	T1169	compound	cell,compartment
R2110	T9653	T9652	agent	by,measured
R2111	T9654	T9655	compound	BAT,gal
R2112	T9655	T9657	compound	gal,expression
R2113	T9656	T9655	punct	-,gal
R2114	T9748	T9717	punct	.,showed
R2115	T9657	T9653	pobj	expression,by
R2116	T9750	T9751	amod	Homozygous,loss
R2117	T9660	T9661	det	A,kidney
R2118	T9661	T9664	nsubj	kidney,showed
R2119	T9662	T9661	amod	negative,kidney
R212	T1172	T1155	punct	.,showed
R2120	T9663	T9661	compound	control,kidney
R2121	T9751	T9752	nsubj	loss,had
R2122	T9665	T9661	punct	", ",kidney
R2123	T9666	T9661	prep	without,kidney
R2124	T9753	T9751	prep	of,loss
R2125	T9667	T9668	det	the,transgene
R2126	T9668	T9666	pobj	transgene,without
R2127	T9669	T9670	compound	BAT,gal
R2128	T9670	T9668	compound	gal,transgene
R2129	T9754	T9755	det	the,gene
R213	T1174	T1175	amod	Confocal,analysis
R2130	T9671	T9670	punct	-,gal
R2131	T9672	T9664	punct	", ",showed
R2132	T9673	T9674	amod	minimal,background
R2133	T9755	T9753	pobj	gene,of
R2134	T9674	T9675	compound	background,staining
R2135	T9756	T9755	compound	Pygo1,gene
R2136	T9675	T9664	dobj	staining,showed
R2137	T9676	T9677	compound	X,gal
R2138	T9757	T9755	advmod	alone,gene
R2139	T9677	T9675	compound	gal,staining
R214	T1175	T1176	nsubj	analysis,showed
R2140	T9678	T9677	punct	-,gal
R2141	T9679	T9680	punct	(,6D
R2142	T9758	T9752	punct	", ",had
R2143	T9680	T9675	parataxis	6D,staining
R2144	T9681	T9680	compound	Figure,6D
R2145	T9759	T9752	advmod	however,had
R2146	T9682	T9680	punct	),6D
R2147	T9683	T9664	punct	", ",showed
R2148	T9760	T9752	punct	", ",had
R2149	T9684	T9685	mark	whereas,showed
R215	T1177	T1175	prep	of,analysis
R2150	T9685	T9664	advcl	showed,showed
R2151	T9761	T9762	det	a,effect
R2152	T9686	T9687	det	a,kidney
R2153	T9687	T9685	nsubj	kidney,showed
R2154	T9688	T9689	compound	BAT,gal
R2155	T9689	T9691	npadvmod	gal,transgenic
R2156	T9762	T9752	dobj	effect,had
R2157	T9690	T9689	punct	-,gal
R2158	T9691	T9687	amod	transgenic,kidney
R2159	T9692	T9687	prep	with,kidney
R216	T1178	T1179	det	the,kidneys
R2160	T9693	T9694	advmod	at,one
R2161	T9694	T9696	nummod	one,gene
R2162	T9695	T9694	advmod	least,one
R2163	T9763	T9762	amod	small,effect
R2164	T9696	T9692	pobj	gene,with
R2165	T9697	T9698	amod	wild,type
R2166	T9698	T9696	compound	type,gene
R2167	T9764	T9762	prep	on,effect
R2168	T9699	T9698	punct	-,type
R2169	T9700	T9696	compound	Pygo2,gene
R217	T1179	T1177	pobj	kidneys,of
R2170	T9765	T9766	compound	reporter,expression
R2171	T9701	T9702	amod	strong,staining
R2172	T9702	T9685	dobj	staining,showed
R2173	T9703	T9704	compound	X,gal
R2174	T9766	T9764	pobj	expression,on
R2175	T9704	T9702	compound	gal,staining
R2176	T9705	T9704	punct	-,gal
R2177	T9706	T9702	prep	in,staining
R2178	T9767	T9768	punct	(,6G
R2179	T9707	T9708	det	the,tree
R218	T1180	T1181	amod	double,mutant
R2180	T9708	T9706	pobj	tree,in
R2181	T9709	T9708	amod	ureteric,tree
R2182	T9768	T9752	parataxis	6G,had
R2183	T9710	T9711	punct	(,6E
R2184	T9711	T9702	parataxis	6E,staining
R2185	T9712	T9711	compound	Figure,6E
R2186	T9713	T9711	punct	),6E
R2187	T9769	T9768	compound	Figure,6G
R2188	T9714	T9664	punct	.,showed
R2189	T9770	T9771	punct	–,H
R219	T1181	T1179	compound	mutant,kidneys
R2190	T9716	T9717	prep	In,showed
R2191	T9718	T9716	pobj	contrast,In
R2192	T9771	T9768	prep	H,6G
R2193	T9719	T9717	punct	", ",showed
R2194	T9720	T9721	nmod	Pygo1,kidneys
R2195	T9721	T9717	nsubj	kidneys,showed
R2196	T9772	T9768	punct	),6G
R2197	T9722	T9720	punct	+,Pygo1
R2198	T9723	T9720	punct	/,Pygo1
R2199	T9724	T9720	punct	-,Pygo1
R22	T966	T964	prep	of,component
R220	T1182	T1176	dobj	disturbance,showed
R2200	T9773	T9752	punct	.,had
R2201	T9725	T9720	punct	/,Pygo1
R2202	T9726	T9720	appos	Pygo2,Pygo1
R2203	T9727	T9726	punct	-,Pygo2
R2204	T9775	T9776	amod	Homozygous,mutation
R2205	T9728	T9726	punct	/,Pygo2
R2206	T9729	T9726	punct	-,Pygo2
R2207	T9730	T9721	compound	E13.5,kidneys
R2208	T9776	T9777	nsubj	mutation,gave
R2209	T9731	T9732	advmod	very,weak
R221	T1183	T1182	prep	of,disturbance
R2210	T9732	T9733	amod	weak,expression
R2211	T9733	T9717	dobj	expression,showed
R2212	T9734	T9733	compound	reporter,expression
R2213	T9735	T9736	punct	(,6F
R2214	T9736	T9733	parataxis	6F,expression
R2215	T9778	T9776	prep	of,mutation
R2216	T9737	T9736	compound	Figure,6F
R2217	T9738	T9736	punct	),6F
R2218	T9739	T9717	punct	", ",showed
R2219	T9740	T9717	advcl	suggesting,showed
R222	T1184	T1185	preconj	both,compartments
R2220	T9741	T9742	det	a,loss
R2221	T9779	T9780	preconj	both,genes
R2222	T9742	T9740	dobj	loss,suggesting
R2223	T9743	T9742	amod	significant,loss
R2224	T9744	T9742	prep	of,loss
R2225	T9780	T9778	pobj	genes,of
R2226	T9745	T9746	amod	canonical,signaling
R2227	T9746	T9744	pobj	signaling,of
R2228	T9781	T9780	det	the,genes
R2229	T9747	T9746	compound	Wnt,signaling
R223	T1185	T1183	pobj	compartments,of
R2230	T9782	T9780	nmod	Pygo1,genes
R2231	T9783	T9782	cc	and,Pygo1
R2232	T9784	T9782	conj	Pygo2,Pygo1
R2233	T9853	T9855	amod	homozygous,mutants
R2234	T9785	T9786	det	a,reduction
R2235	T9854	T9853	punct	-,homozygous
R2236	T9855	T9856	nsubj	mutants,showed
R2237	T9786	T9777	dobj	reduction,gave
R2238	T9856	T9831	conj	showed,was
R2239	T9857	T9856	dobj	loss,showed
R224	T1186	T1185	det	the,compartments
R2240	T9787	T9788	advmod	more,dramatic
R2241	T9858	T9857	prep	of,loss
R2242	T9859	T9860	compound	X,gal
R2243	T9788	T9786	amod	dramatic,reduction
R2244	T9860	T9862	compound	gal,staining
R2245	T9861	T9860	punct	-,gal
R2246	T9862	T9858	pobj	staining,of
R2247	T9789	T9786	prep	of,reduction
R2248	T9863	T9856	prep	in,showed
R2249	T9864	T9865	det	the,ducts
R225	T1187	T1188	amod	ureteric,bud
R2250	T9865	T9863	pobj	ducts,in
R2251	T9790	T9791	compound	BAT,gal
R2252	T9866	T9865	amod	paramesonephric,ducts
R2253	T9867	T9868	punct	(,6K
R2254	T9868	T9865	parataxis	6K,ducts
R2255	T9791	T9793	compound	gal,expression
R2256	T9869	T9868	compound	Figure,6K
R2257	T9870	T9868	punct	),6K
R2258	T9871	T9856	punct	", ",showed
R2259	T9792	T9791	punct	-,gal
R226	T1188	T1189	npadvmod	bud,derived
R2260	T9872	T9873	mark	whereas,showed
R2261	T9873	T9856	advcl	showed,showed
R2262	T9793	T9789	pobj	expression,of
R2263	T9874	T9875	nmod	Pygo1,mice
R2264	T9875	T9873	nsubj	mice,showed
R2265	T9876	T9874	punct	-,Pygo1
R2266	T9877	T9874	punct	/,Pygo1
R2267	T9878	T9874	punct	-,Pygo1
R2268	T9879	T9874	punct	/,Pygo1
R2269	T9794	T9786	prep	than,reduction
R227	T1189	T1185	amod	derived,compartments
R2270	T9880	T9874	appos	Pygo2,Pygo1
R2271	T9881	T9880	punct	+,Pygo2
R2272	T9882	T9880	punct	/,Pygo2
R2273	T9883	T9880	punct	-,Pygo2
R2274	T9795	T9794	pobj	loss,than
R2275	T9884	T9874	cc	and,Pygo1
R2276	T9885	T9874	conj	Pygo1,Pygo1
R2277	T9796	T9795	prep	of,loss
R2278	T9886	T9885	punct	+,Pygo1
R2279	T9887	T9885	punct	/,Pygo1
R228	T1190	T1188	cc	and,bud
R2280	T9888	T9885	punct	-,Pygo1
R2281	T9797	T9796	pobj	Pygo2,of
R2282	T9889	T9885	punct	/,Pygo1
R2283	T9890	T9885	appos	Pygo2,Pygo1
R2284	T9891	T9890	punct	+,Pygo2
R2285	T9892	T9890	punct	/,Pygo2
R2286	T9798	T9795	advmod	alone,loss
R2287	T9893	T9890	punct	-,Pygo2
R2288	T9894	T9895	amod	normal,levels
R2289	T9895	T9873	dobj	levels,showed
R229	T1191	T1192	amod	metanephric,mesenchyme
R2290	T9799	T9800	punct	(,L
R2291	T9896	T9895	prep	of,levels
R2292	T9897	T9898	compound	BAT,gal
R2293	T9898	T9900	compound	gal,expression
R2294	T9800	T9786	parataxis	L,reduction
R2295	T9899	T9898	punct	-,gal
R2296	T9900	T9896	pobj	expression,of
R2297	T9901	T9902	punct	(,J
R2298	T9801	T9800	nmod	Figure,L
R2299	T9902	T9873	parataxis	J,showed
R23	T967	T968	det	the,complex
R230	T1192	T1188	conj	mesenchyme,bud
R2300	T9903	T9902	nmod	Figure,J
R2301	T9802	T9800	nmod	6F,L
R2302	T9904	T9902	nmod	6G,J
R2303	T9905	T9902	punct	", ",J
R2304	T9906	T9902	nmod	H,J
R2305	T9803	T9800	punct	", ",L
R2306	T9907	T9902	punct	", ",J
R2307	T9908	T9902	punct	),J
R2308	T9909	T9856	punct	.,showed
R2309	T9804	T9800	punct	),L
R231	T1193	T1189	punct	-,derived
R2310	T9911	T9912	compound	BAT,gal
R2311	T9912	T9914	compound	gal,reporter
R2312	T9913	T9912	punct	-,gal
R2313	T9805	T9777	punct	.,gave
R2314	T9914	T9915	compound	reporter,analysis
R2315	T9915	T9916	nsubj	analysis,identified
R2316	T9917	T9915	prep	of,analysis
R2317	T9918	T9919	det	the,mutants
R2318	T9807	T9808	det	The,genes
R2319	T9919	T9917	pobj	mutants,of
R232	T1194	T1176	punct	.,showed
R2320	T9920	T9919	compound	Pygo,mutants
R2321	T9921	T9915	prep	at,analysis
R2322	T9922	T9923	det	a,stage
R2323	T9923	T9921	pobj	stage,at
R2324	T9924	T9923	advmod	later,stage
R2325	T9808	T9812	nsubjpass	genes,required
R2326	T9925	T9923	amod	developmental,stage
R2327	T9926	T9923	punct	", ",stage
R2328	T9927	T9923	appos	E18.5,stage
R2329	T9809	T9808	nmod	Pygo1,genes
R233	T1196	T1197	amod	Branching,morphogenesis
R2330	T9928	T9916	punct	", ",identified
R2331	T9929	T9916	advmod	also,identified
R2332	T9930	T9931	det	a,decrease
R2333	T9810	T9809	cc	and,Pygo1
R2334	T9811	T9809	conj	Pygo2,Pygo1
R2335	T9931	T9916	dobj	decrease,identified
R2336	T9932	T9931	amod	significant,decrease
R2337	T9813	T9812	auxpass	were,required
R2338	T9933	T9931	prep	in,decrease
R2339	T9934	T9935	amod	canonical,signaling
R234	T1197	T1198	nsubjpass	morphogenesis,altered
R2340	T9935	T9933	pobj	signaling,in
R2341	T9936	T9935	compound	Wnt,signaling
R2342	T9937	T9931	prep	in,decrease
R2343	T9814	T9812	advmod	also,required
R2344	T9938	T9939	det	the,branches
R2345	T9939	T9937	pobj	branches,in
R2346	T9815	T9812	prep	for,required
R2347	T9940	T9939	amod	cortical,branches
R2348	T9941	T9939	amod	ureteric,branches
R2349	T9942	T9939	cc	and,branches
R235	T1199	T1197	prep	of,morphogenesis
R2350	T9816	T9817	compound	BAT,gal
R2351	T9943	T9944	amod	renal,pelvis
R2352	T9944	T9939	conj	pelvis,branches
R2353	T9945	T9939	prep	of,branches
R2354	T9817	T9819	compound	gal,reporter
R2355	T9946	T9947	det	the,kidney
R2356	T9947	T9945	pobj	kidney,of
R2357	T9948	T9947	amod	developing,kidney
R2358	T9818	T9817	punct	-,gal
R2359	T9949	T9950	punct	(,6M
R236	T1200	T1201	det	the,bud
R2360	T9950	T9916	parataxis	6M,identified
R2361	T9951	T9950	compound	Figure,6M
R2362	T9819	T9820	compound	reporter,expression
R2363	T9952	T9953	punct	–,O
R2364	T9953	T9950	prep	O,6M
R2365	T9820	T9815	pobj	expression,for
R2366	T9954	T9950	punct	),6M
R2367	T9955	T9916	punct	.,identified
R2368	T9957	T9958	amod	Cortical,staining
R2369	T9821	T9820	prep	in,expression
R237	T1201	T1199	pobj	bud,of
R2370	T9822	T9823	det	the,ducts
R2371	T9958	T9962	nsubjpass	staining,seen
R2372	T9823	T9821	pobj	ducts,in
R2373	T9959	T9960	compound	X,gal
R2374	T9960	T9958	compound	gal,staining
R2375	T9961	T9960	punct	-,gal
R2376	T9824	T9823	amod	paramesonephric,ducts
R2377	T9963	T9962	auxpass	was,seen
R2378	T9964	T9962	prep	in,seen
R2379	T9825	T9826	punct	(,Mullerian
R238	T1202	T1201	amod	ureteric,bud
R2380	T9965	T9966	det	the,branches
R2381	T9966	T9964	pobj	branches,in
R2382	T9967	T9966	amod	ureteric,branches
R2383	T9826	T9824	parataxis	Mullerian,paramesonephric
R2384	T9968	T9966	prep	of,branches
R2385	T9969	T9970	det	a,kidney
R2386	T9970	T9968	pobj	kidney,of
R2387	T9827	T9826	punct	),Mullerian
R2388	T9971	T9970	nmod	Pygo1,kidney
R2389	T9972	T9971	punct	+,Pygo1
R239	T1203	T1198	auxpass	was,altered
R2390	T9973	T9971	punct	/,Pygo1
R2391	T9828	T9812	punct	.,required
R2392	T9974	T9971	punct	-,Pygo1
R2393	T9975	T9971	punct	/,Pygo1
R2394	T9976	T9971	appos	Pygo2,Pygo1
R2395	T9830	T9831	prep	In,was
R2396	T9977	T9976	punct	+,Pygo2
R2397	T9978	T9976	punct	/,Pygo2
R2398	T9979	T9976	punct	-,Pygo2
R2399	T9980	T9981	punct	(,left
R24	T968	T966	pobj	complex,of
R240	T1204	T1198	punct	", ",altered
R2400	T9981	T9962	parataxis	left,seen
R2401	T9832	T9833	nmod	Pygo2,mice
R2402	T9982	T9983	compound	Figure,6M
R2403	T9983	T9981	dep	6M,left
R2404	T9984	T9981	punct	", ",left
R2405	T9985	T9981	punct	),left
R2406	T9833	T9830	pobj	mice,In
R2407	T9986	T9962	punct	", ",seen
R2408	T9987	T9962	cc	but,seen
R2409	T9988	T9962	conj	was,seen
R241	T1205	T1198	prep	with,altered
R2410	T9834	T9832	punct	-,Pygo2
R2411	T9989	T9990	advmod	completely,absent
R2412	T9990	T9988	acomp	absent,was
R2413	T9991	T9988	prep	in,was
R2414	T9835	T9832	punct	/,Pygo2
R2415	T9992	T9993	det	the,cortex
R2416	T9836	T9832	punct	-,Pygo2
R2417	T9993	T9991	pobj	cortex,in
R2418	T9837	T9831	punct	", ",was
R2419	T9994	T9993	prep	of,cortex
R242	T1206	T1207	amod	expanded,tips
R2420	T9995	T9996	det	a,kidney
R2421	T9996	T9994	pobj	kidney,of
R2422	T9997	T9996	nmod	Pygo1,kidney
R2423	T9838	T9831	expl	there,was
R2424	T9998	T9997	punct	+,Pygo1
R2425	T9999	T9997	punct	/,Pygo1
R2426	T10000	T9997	punct	-,Pygo1
R2427	T9839	T9840	det	a,loss
R2428	T10001	T9997	punct	/,Pygo1
R2429	T10002	T9997	appos	Pygo2,Pygo1
R243	T1207	T1205	pobj	tips,with
R2430	T10003	T10002	punct	-,Pygo2
R2431	T9840	T9831	attr	loss,was
R2432	T10004	T10002	punct	/,Pygo2
R2433	T10005	T10002	punct	-,Pygo2
R2434	T10006	T10007	punct	(,right
R2435	T9841	T9840	amod	significant,loss
R2436	T10007	T9988	parataxis	right,was
R2437	T10008	T10009	compound	Figure,6M
R2438	T10009	T10007	dep	6M,right
R2439	T9842	T9840	prep	of,loss
R244	T1208	T1207	cc	and,tips
R2440	T10010	T10007	punct	", ",right
R2441	T10011	T10007	punct	),right
R2442	T10012	T9962	punct	.,seen
R2443	T9843	T9844	compound	reporter,expression
R2444	T10014	T10015	nsubj	Bisection,revealed
R2445	T9844	T9842	pobj	expression,of
R2446	T10016	T10017	det	a,loss
R2447	T9845	T9846	punct	(,shown
R2448	T9846	T9831	parataxis	shown,was
R2449	T10017	T10015	dobj	loss,revealed
R245	T1209	T1210	amod	reduced,density
R2450	T10018	T10017	amod	significant,loss
R2451	T10019	T10017	prep	of,loss
R2452	T9847	T9846	nsubj	data,shown
R2453	T10020	T10021	compound	X,gal
R2454	T10021	T10023	npadvmod	gal,staining
R2455	T10022	T10021	punct	-,gal
R2456	T9848	T9846	neg	not,shown
R2457	T10023	T10024	amod	staining,cells
R2458	T10024	T10019	pobj	cells,of
R2459	T9849	T9846	punct	),shown
R246	T1210	T1207	conj	density,tips
R2460	T10025	T10017	prep	in,loss
R2461	T10026	T10027	det	the,ducts
R2462	T10027	T10025	pobj	ducts,in
R2463	T9850	T9831	punct	", ",was
R2464	T10028	T10027	amod	collecting,ducts
R2465	T10029	T10027	cc	and,ducts
R2466	T10030	T10031	amod	renal,pelvis
R2467	T10031	T10027	conj	pelvis,ducts
R2468	T9851	T9831	cc	and,was
R2469	T10032	T10027	prep	of,ducts
R247	T1211	T1210	compound	tip,density
R2470	T10033	T10034	det	the,kidney
R2471	T10034	T10032	pobj	kidney,of
R2472	T10035	T10036	npadvmod	Pygo2,null
R2473	T10036	T10034	amod	null,kidney
R2474	T9852	T9853	amod	double,homozygous
R2475	T10037	T10017	prep	compared,loss
R2476	T10038	T10037	prep	with,compared
R2477	T10039	T10040	compound	control,littermates
R2478	T10040	T10038	pobj	littermates,with
R2479	T10041	T10042	punct	(,6N
R248	T1212	T1198	punct	", ",altered
R2480	T10063	T10064	punct	(,left
R2481	T10042	T10015	parataxis	6N,revealed
R2482	T10043	T10042	compound	Figure,6N
R2483	T10044	T10042	punct	),6N
R2484	T10064	T10053	parataxis	left,Pygo1
R2485	T10045	T10015	punct	.,revealed
R2486	T10047	T10048	nmod	Side,comparison
R2487	T10048	T10051	nsubj	comparison,suggested
R2488	T10065	T10066	compound	Figure,6O
R2489	T10049	T10047	prep	by,Side
R249	T1213	T1214	advmod	probably,contributing
R2490	T10050	T10049	pobj	side,by
R2491	T10066	T10064	dep	6O,left
R2492	T10052	T10048	prep	of,comparison
R2493	T10053	T10054	nmod	Pygo1,kidneys
R2494	T10067	T10064	punct	", ",left
R2495	T10068	T10064	punct	),left
R2496	T10054	T10052	pobj	kidneys,of
R2497	T10055	T10053	punct	+,Pygo1
R2498	T10069	T10053	punct	", ",Pygo1
R2499	T10056	T10053	punct	/,Pygo1
R25	T969	T970	nmod	Armadillo,factor
R250	T1214	T1198	advcl	contributing,altered
R2500	T10057	T10053	punct	+,Pygo1
R2501	T10058	T10053	punct	/,Pygo1
R2502	T10070	T10053	conj	Pygo1,Pygo1
R2503	T10059	T10053	appos	Pygo2,Pygo1
R2504	T10060	T10059	punct	+,Pygo2
R2505	T10061	T10059	punct	/,Pygo2
R2506	T10071	T10070	punct	-,Pygo1
R2507	T10062	T10059	punct	-,Pygo2
R2508	T10072	T10070	punct	/,Pygo1
R2509	T10073	T10070	punct	-,Pygo1
R251	T1215	T1214	prep	to,contributing
R2510	T10168	T10166	prep	of,Loss
R2511	T10169	T10170	det	the,gene
R2512	T10074	T10070	punct	/,Pygo1
R2513	T10170	T10168	pobj	gene,of
R2514	T10171	T10170	compound	Pygo2,gene
R2515	T10172	T10166	punct	(,Loss
R2516	T10075	T10070	appos	Pygo2,Pygo1
R2517	T10173	T10166	appos	Pygo1,Loss
R2518	T10174	T10173	punct	+,Pygo1
R2519	T10076	T10075	punct	+,Pygo2
R252	T1216	T1217	det	the,size
R2520	T10175	T10173	punct	/,Pygo1
R2521	T10176	T10173	punct	-,Pygo1
R2522	T10177	T10173	punct	/,Pygo1
R2523	T10077	T10075	punct	/,Pygo2
R2524	T10178	T10173	appos	Pygo2,Pygo1
R2525	T10179	T10178	punct	-,Pygo2
R2526	T10180	T10178	punct	/,Pygo2
R2527	T10181	T10178	punct	-,Pygo2
R2528	T10182	T10173	cc	and,Pygo1
R2529	T10078	T10075	punct	-,Pygo2
R253	T1217	T1215	pobj	size,to
R2530	T10183	T10173	conj	Pygo1,Pygo1
R2531	T10184	T10183	punct	-,Pygo1
R2532	T10079	T10080	punct	(,middle
R2533	T10080	T10070	parataxis	middle,Pygo1
R2534	T10185	T10183	punct	/,Pygo1
R2535	T10186	T10183	punct	-,Pygo1
R2536	T10187	T10183	punct	/,Pygo1
R2537	T10081	T10082	compound	Figure,6O
R2538	T10188	T10183	appos	Pygo2,Pygo1
R2539	T10189	T10188	punct	-,Pygo2
R254	T1218	T1217	amod	smaller,size
R2540	T10082	T10080	dep	6O,middle
R2541	T10190	T10188	punct	/,Pygo2
R2542	T10191	T10188	punct	-,Pygo2
R2543	T10192	T10167	punct	),gave
R2544	T10083	T10080	punct	", ",middle
R2545	T10193	T10194	amod	greater,90
R2546	T10194	T10196	nummod	90,%
R2547	T10084	T10080	punct	),middle
R2548	T10195	T10194	quantmod	than,90
R2549	T10196	T10197	compound	%,reduction
R255	T1219	T1217	prep	of,size
R2550	T10197	T10167	dobj	reduction,gave
R2551	T10085	T10070	punct	", ",Pygo1
R2552	T10198	T10197	prep	in,reduction
R2553	T10199	T10200	compound	BAT,gal
R2554	T10200	T10202	compound	gal,expression
R2555	T10086	T10070	cc	and,Pygo1
R2556	T10201	T10200	punct	-,gal
R2557	T10202	T10198	pobj	expression,in
R2558	T10203	T10167	punct	.,gave
R2559	T10087	T10070	conj	Pygo1,Pygo1
R256	T1220	T1221	det	the,kidney
R2560	T10205	T10206	nsubj	Loss,result
R2561	T10088	T10087	punct	-,Pygo1
R2562	T10207	T10205	prep	of,Loss
R2563	T10208	T10207	pobj	Pygo1,of
R2564	T10209	T10205	advmod	alone,Loss
R2565	T10089	T10087	punct	/,Pygo1
R2566	T10210	T10205	punct	(,Loss
R2567	T10211	T10205	appos	Pygo1,Loss
R2568	T10212	T10211	punct	-,Pygo1
R2569	T10090	T10087	punct	-,Pygo1
R257	T1221	T1219	pobj	kidney,of
R2570	T10213	T10211	punct	/,Pygo1
R2571	T10214	T10211	punct	-,Pygo1
R2572	T10215	T10211	punct	/,Pygo1
R2573	T10216	T10211	appos	Pygo2,Pygo1
R2574	T10091	T10087	punct	/,Pygo1
R2575	T10217	T10216	punct	+,Pygo2
R2576	T10218	T10216	punct	/,Pygo2
R2577	T10219	T10216	punct	+,Pygo2
R2578	T10220	T10206	punct	),result
R2579	T10221	T10206	aux	did,result
R258	T1222	T1221	compound	mutant,kidney
R2580	T10222	T10206	neg	not,result
R2581	T10092	T10087	appos	Pygo2,Pygo1
R2582	T10223	T10206	prep	in,result
R2583	T10224	T10225	det	a,change
R2584	T10093	T10092	punct	-,Pygo2
R2585	T10225	T10223	pobj	change,in
R2586	T10226	T10225	amod	significant,change
R2587	T10227	T10206	punct	.,result
R2588	T10094	T10092	punct	/,Pygo2
R2589	T10229	T10230	advmod	Interestingly,showed
R259	T1223	T1198	punct	.,altered
R2590	T10095	T10092	punct	-,Pygo2
R2591	T10231	T10230	punct	", ",showed
R2592	T10232	T10230	advmod	however,showed
R2593	T10233	T10230	punct	", ",showed
R2594	T10096	T10097	punct	(,right
R2595	T10234	T10235	det	the,Pygo1
R2596	T10235	T10230	nsubj	Pygo1,showed
R2597	T10236	T10235	punct	-,Pygo1
R2598	T10097	T10087	parataxis	right,Pygo1
R2599	T10237	T10235	punct	/,Pygo1
R26	T970	T968	compound	factor,complex
R260	T1225	T1226	prep	In,was
R2600	T10238	T10235	punct	-,Pygo1
R2601	T10239	T10235	punct	/,Pygo1
R2602	T10240	T10235	appos	Pygo2,Pygo1
R2603	T10098	T10099	compound	Figure,6O
R2604	T10241	T10240	punct	+,Pygo2
R2605	T10242	T10240	punct	/,Pygo2
R2606	T10243	T10240	punct	-,Pygo2
R2607	T10099	T10097	dep	6O,right
R2608	T10244	T10245	advmod	only,50
R2609	T10245	T10246	nummod	50,%
R261	T1227	T1225	pobj	addition,In
R2610	T10246	T10230	dobj	%,showed
R2611	T10100	T10097	punct	", ",right
R2612	T10247	T10246	prep	of,%
R2613	T10248	T10249	amod	wild,type
R2614	T10249	T10251	compound	type,expression
R2615	T10250	T10249	punct	-,type
R2616	T10101	T10097	punct	),right
R2617	T10251	T10247	pobj	expression,of
R2618	T10252	T10253	compound	BAT,gal
R2619	T10253	T10251	compound	gal,expression
R262	T1228	T1226	punct	", ",was
R2620	T10102	T10054	compound	E18.5,kidneys
R2621	T10254	T10253	punct	-,gal
R2622	T10255	T10230	punct	", ",showed
R2623	T10256	T10230	advcl	suggesting,showed
R2624	T10103	T10104	det	a,role
R2625	T10257	T10258	det	a,contribution
R2626	T10258	T10256	dobj	contribution,suggesting
R2627	T10104	T10051	dobj	role,suggested
R2628	T10259	T10258	amod	minor,contribution
R2629	T10260	T10258	prep	by,contribution
R263	T1229	T1226	expl	there,was
R2630	T10261	T10260	pobj	Pygo1,by
R2631	T10262	T10258	prep	in,contribution
R2632	T10263	T10264	amod	canonical,signaling
R2633	T10264	T10262	pobj	signaling,in
R2634	T10265	T10264	compound	Wnt,signaling
R2635	T10105	T10104	amod	significant,role
R2636	T10266	T10230	punct	.,showed
R2637	T10268	T10269	mark	Although,decreased
R2638	T10106	T10104	prep	for,role
R2639	T10107	T10108	det	the,gene
R264	T1230	T1231	det	an,expansion
R2640	T10269	T10275	advcl	decreased,revealed
R2641	T10270	T10271	compound	BAT,gal
R2642	T10108	T10106	pobj	gene,for
R2643	T10271	T10273	compound	gal,expression
R2644	T10272	T10271	punct	-,gal
R2645	T10109	T10108	compound	Pygo2,gene
R2646	T10110	T10104	prep	in,role
R2647	T10111	T10112	amod	canonical,signaling
R2648	T10273	T10269	nsubjpass	expression,decreased
R2649	T10112	T10110	pobj	signaling,in
R265	T1231	T1226	attr	expansion,was
R2650	T10274	T10269	auxpass	was,decreased
R2651	T10276	T10269	prep	in,decreased
R2652	T10277	T10278	det	the,kidney
R2653	T10113	T10112	compound	Wnt,signaling
R2654	T10278	T10276	pobj	kidney,in
R2655	T10279	T10278	nmod	E18.5,kidney
R2656	T10280	T10278	nmod	Pygo1,kidney
R2657	T10114	T10104	prep	in,role
R2658	T10281	T10280	punct	-,Pygo1
R2659	T10282	T10280	punct	/,Pygo1
R266	T1232	T1231	prep	of,expansion
R2660	T10283	T10280	punct	-,Pygo1
R2661	T10115	T10116	det	the,tree
R2662	T10284	T10280	punct	/,Pygo1
R2663	T10285	T10280	appos	Pygo2,Pygo1
R2664	T10116	T10114	pobj	tree,in
R2665	T10286	T10285	punct	+,Pygo2
R2666	T10287	T10285	punct	/,Pygo2
R2667	T10288	T10285	punct	-,Pygo2
R2668	T10117	T10116	amod	ureteric,tree
R2669	T10289	T10275	punct	", ",revealed
R267	T1233	T1234	det	the,zone
R2670	T10290	T10291	amod	confocal,analysis
R2671	T10291	T10275	nsubj	analysis,revealed
R2672	T10118	T10116	cc	and,tree
R2673	T10292	T10291	prep	of,analysis
R2674	T10293	T10292	pobj	kidneys,of
R2675	T10294	T10293	prep	with,kidneys
R2676	T10119	T10120	poss	its,derivatives
R2677	T10295	T10296	det	this,genotype
R2678	T10296	T10294	pobj	genotype,with
R2679	T10297	T10298	det	no,tips
R268	T1234	T1232	pobj	zone,of
R2680	T10120	T10116	conj	derivatives,tree
R2681	T10298	T10275	dobj	tips,revealed
R2682	T10299	T10298	amod	dilated,tips
R2683	T10300	T10298	amod	ureteric,tips
R2684	T10301	T10298	cc	or,tips
R2685	T10302	T10303	amod	significant,changes
R2686	T10303	T10298	conj	changes,tips
R2687	T10121	T10051	punct	.,suggested
R2688	T10304	T10303	prep	in,changes
R2689	T10305	T10306	amod	ureteric,tip
R269	T1235	T1234	prep	of,zone
R2690	T10306	T10307	compound	tip,number
R2691	T10123	T10124	prep	In,performed
R2692	T10307	T10304	pobj	number,in
R2693	T10308	T10307	prep	per,number
R2694	T10309	T10308	pobj	area,per
R2695	T10310	T10303	prep	compared,changes
R2696	T10125	T10123	pobj	order,In
R2697	T10311	T10310	prep	with,compared
R2698	T10312	T10313	nmod	Pygo1,kidneys
R2699	T10313	T10311	pobj	kidneys,with
R27	T971	T972	punct	(,catenin
R270	T1236	T1237	amod	condensed,mesenchyme
R2700	T10314	T10312	punct	-,Pygo1
R2701	T10126	T10127	aux	to,validate
R2702	T10315	T10312	punct	/,Pygo1
R2703	T10316	T10312	punct	-,Pygo1
R2704	T10317	T10312	punct	/,Pygo1
R2705	T10127	T10125	acl	validate,order
R2706	T10318	T10312	appos	Pygo2,Pygo1
R2707	T10319	T10318	punct	+,Pygo2
R2708	T10320	T10318	punct	/,Pygo2
R2709	T10128	T10127	cc	and,validate
R271	T1237	T1235	pobj	mesenchyme,of
R2710	T10321	T10318	punct	+,Pygo2
R2711	T10322	T10323	punct	(,shown
R2712	T10129	T10127	conj	quantify,validate
R2713	T10323	T10275	parataxis	shown,revealed
R2714	T10324	T10323	nsubj	data,shown
R2715	T10325	T10323	neg	not,shown
R2716	T10326	T10323	punct	),shown
R2717	T10130	T10131	det	the,changes
R2718	T10327	T10275	punct	.,revealed
R2719	T10131	T10129	dobj	changes,quantify
R272	T1238	T1234	acl	capping,zone
R2720	T10329	T10330	advmod	Collectively,suggest
R2721	T10331	T10330	punct	", ",suggest
R2722	T10132	T10133	compound	BAT,gal
R2723	T10332	T10333	det	these,results
R2724	T10333	T10330	nsubj	results,suggest
R2725	T10334	T10335	compound	BAT,gal
R2726	T10335	T10337	compound	gal,reporter
R2727	T10133	T10135	compound	gal,reporter
R2728	T10336	T10335	punct	-,gal
R2729	T10337	T10333	compound	reporter,results
R273	T1239	T1240	det	the,bud
R2730	T10338	T10339	det	a,role
R2731	T10134	T10133	punct	-,gal
R2732	T10339	T10330	dobj	role,suggest
R2733	T10340	T10339	amod	significant,role
R2734	T10341	T10339	prep	for,role
R2735	T10342	T10343	det	the,genes
R2736	T10343	T10341	pobj	genes,for
R2737	T10344	T10343	nmod	Pygo1,genes
R2738	T10135	T10136	compound	reporter,expression
R2739	T10345	T10344	cc	and,Pygo1
R274	T1240	T1238	dobj	bud,capping
R2740	T10346	T10344	conj	Pygo2,Pygo1
R2741	T10347	T10339	prep	in,role
R2742	T10348	T10349	amod	canonical,signaling
R2743	T10136	T10131	compound	expression,changes
R2744	T10349	T10347	pobj	signaling,in
R2745	T10350	T10349	compound	Wnt,signaling
R2746	T10351	T10339	prep	during,role
R2747	T10137	T10131	prep	in,changes
R2748	T10352	T10351	pobj	development,during
R2749	T10353	T10352	prep	of,development
R275	T1241	T1240	amod	ureteric,bud
R2750	T10138	T10139	det	the,null
R2751	T10354	T10355	det	the,tree
R2752	T10139	T10137	pobj	null,in
R2753	T10140	T10139	compound	Pygo2,null
R2754	T10141	T10139	cc	and,null
R2755	T10355	T10353	pobj	tree,of
R2756	T10142	T10143	compound	Pygo1,Pygo2
R2757	T10356	T10355	amod	ureteric,tree
R2758	T10357	T10355	prep	of,tree
R2759	T10143	T10145	compound	Pygo2,nulls
R276	T1242	T1226	punct	.,was
R2760	T10358	T10359	det	the,kidney
R2761	T10359	T10357	pobj	kidney,of
R2762	T10360	T10330	punct	.,suggest
R2763	T10144	T10143	punct	/,Pygo2
R2764	T10145	T10139	conj	nulls,null
R2765	T10146	T10124	punct	", ",performed
R2766	T10147	T10124	nsubj	we,performed
R2767	T10148	T10149	compound	ELISA,measurements
R2768	T10149	T10124	dobj	measurements,performed
R2769	T10150	T10149	prep	of,measurements
R277	T1244	T1245	compound	Nephron,formation
R2770	T10151	T10152	npadvmod	transgene,specific
R2771	T10152	T10153	amod	specific,levels
R2772	T10362	T10363	advmod	Interestingly,showed
R2773	T10153	T10150	pobj	levels,of
R2774	T10364	T10363	punct	", ",showed
R2775	T10154	T10155	compound	β,galactosidase
R2776	T10365	T10363	advmod	however,showed
R2777	T10366	T10363	punct	", ",showed
R2778	T10155	T10153	compound	galactosidase,levels
R2779	T10367	T10368	advmod	even,in
R278	T1245	T1246	nsubj	formation,proceeded
R2780	T10368	T10363	prep	in,showed
R2781	T10369	T10370	amod	wild,type
R2782	T10156	T10155	punct	-,galactosidase
R2783	T10370	T10372	compound	type,mice
R2784	T10371	T10370	punct	-,type
R2785	T10372	T10368	pobj	mice,in
R2786	T10373	T10374	det	the,reporter
R2787	T10374	T10363	nsubj	reporter,showed
R2788	T10157	T10149	prep	in,measurements
R2789	T10375	T10376	compound	BAT,gal
R279	T1247	T1246	punct	", ",proceeded
R2790	T10376	T10374	compound	gal,reporter
R2791	T10377	T10376	punct	-,gal
R2792	T10378	T10379	det	no,expression
R2793	T10158	T10159	compound	E18.5,kidneys
R2794	T10379	T10363	dobj	expression,showed
R2795	T10380	T10379	prep	in,expression
R2796	T10159	T10157	pobj	kidneys,in
R2797	T10381	T10382	det	the,mesenchyme
R2798	T10382	T10380	pobj	mesenchyme,in
R2799	T10383	T10382	amod	developing,mesenchyme
R28	T972	T969	parataxis	catenin,Armadillo
R280	T1248	T1246	advmod	however,proceeded
R2800	T10160	T10161	punct	(,Figure
R2801	T10384	T10382	amod	metanephric,mesenchyme
R2802	T10385	T10382	punct	", ",mesenchyme
R2803	T10386	T10382	cc	or,mesenchyme
R2804	T10161	T10124	parataxis	Figure,performed
R2805	T10387	T10388	amod	metanephric,mesenchyme
R2806	T10388	T10389	npadvmod	mesenchyme,derived
R2807	T10389	T10391	amod	derived,structures
R2808	T10162	T10161	nummod	7,Figure
R2809	T10390	T10389	punct	-,derived
R281	T1249	T1246	punct	", ",proceeded
R2810	T10391	T10382	conj	structures,mesenchyme
R2811	T10163	T10161	punct	),Figure
R2812	T10392	T10391	punct	", ",structures
R2813	T10393	T10394	amod	such,as
R2814	T10394	T10391	prep	as,structures
R2815	T10395	T10396	amod	renal,vesicles
R2816	T10164	T10124	punct	.,performed
R2817	T10396	T10394	pobj	vesicles,as
R2818	T10397	T10396	punct	", ",vesicles
R2819	T10166	T10167	nsubj	Loss,gave
R282	T1250	T1246	advmod	normally,proceeded
R2820	T10398	T10399	npadvmod	S,shaped
R2821	T10399	T10401	amod	shaped,bodies
R2822	T10400	T10399	punct	-,shaped
R2823	T10401	T10396	conj	bodies,vesicles
R2824	T10402	T10401	punct	", ",bodies
R2825	T10460	T10461	amod	ureteric,bud
R2826	T10403	T10401	conj	tubules,bodies
R2827	T10404	T10403	punct	", ",tubules
R2828	T10405	T10403	cc	and,tubules
R2829	T10406	T10403	conj	glomeruli,tubules
R283	T1251	T1246	punct	.,proceeded
R2830	T10461	T10462	compound	bud,expression
R2831	T10407	T10363	punct	.,showed
R2832	T10409	T10410	nsubjpass	This,reported
R2833	T10462	T10457	nsubj	expression,rescue
R2834	T10411	T10410	aux	has,reported
R2835	T10412	T10410	auxpass	been,reported
R2836	T10463	T10462	prep	of,expression
R2837	T10413	T10410	advmod	previously,reported
R2838	T10414	T10410	punct	", ",reported
R2839	T10415	T10410	cc	and,reported
R284	T1253	T1254	compound	Microarray,analysis
R2840	T10416	T10417	auxpass	was,interpreted
R2841	T10417	T10410	conj	interpreted,reported
R2842	T10464	T10463	pobj	Wnt1,of
R2843	T10418	T10419	aux	to,indicate
R2844	T10419	T10417	xcomp	indicate,interpreted
R2845	T10420	T10421	det	the,absence
R2846	T10465	T10462	punct	", ",expression
R2847	T10421	T10419	dobj	absence,indicate
R2848	T10422	T10421	prep	of,absence
R2849	T10423	T10424	amod	canonical,signaling
R285	T1254	T1255	nsubj	analysis,showed
R2850	T10424	T10422	pobj	signaling,of
R2851	T10466	T10462	acl	thought,expression
R2852	T10425	T10424	compound	Wnt,signaling
R2853	T10426	T10421	prep	in,absence
R2854	T10467	T10468	aux	to,act
R2855	T10427	T10428	det	the,mesenchyme
R2856	T10428	T10426	pobj	mesenchyme,in
R2857	T10429	T10428	amod	metanephric,mesenchyme
R2858	T10430	T10431	punct	[,21
R2859	T10468	T10466	xcomp	act,thought
R286	T1256	T1257	amod	changed,expression
R2860	T10431	T10419	parataxis	21,indicate
R2861	T10432	T10431	punct	],21
R2862	T10469	T10468	prep	through,act
R2863	T10433	T10410	punct	.,reported
R2864	T10435	T10436	nsubj	Results,argue
R2865	T10470	T10471	amod	canonical,signaling
R2866	T10437	T10435	prep	from,Results
R2867	T10471	T10469	pobj	signaling,through
R2868	T10438	T10439	amod	other,studies
R2869	T10439	T10437	pobj	studies,from
R287	T1257	T1255	dobj	expression,showed
R2870	T10440	T10436	punct	", ",argue
R2871	T10441	T10436	advmod	however,argue
R2872	T10472	T10471	compound	Wnt,signaling
R2873	T10442	T10436	punct	", ",argue
R2874	T10443	T10436	prep	for,argue
R2875	T10444	T10445	det	the,presence
R2876	T10473	T10457	punct	", ",rescue
R2877	T10445	T10443	pobj	presence,for
R2878	T10446	T10445	prep	of,presence
R2879	T10447	T10448	amod	canonical,signaling
R288	T1258	T1257	prep	of,expression
R2880	T10474	T10457	aux	can,rescue
R2881	T10448	T10446	pobj	signaling,of
R2882	T10449	T10448	compound	Wnt,signaling
R2883	T10450	T10445	prep	in,presence
R2884	T10451	T10452	det	the,mesenchyme
R2885	T10475	T10476	compound	Wnt9b,mutants
R2886	T10452	T10450	pobj	mesenchyme,in
R2887	T10453	T10452	amod	metanephric,mesenchyme
R2888	T10454	T10436	punct	.,argue
R2889	T10476	T10457	dobj	mutants,rescue
R289	T1259	T1260	amod	several,genes
R2890	T10456	T10457	prep	For,rescue
R2891	T10458	T10456	pobj	example,For
R2892	T10459	T10457	punct	", ",rescue
R2893	T10477	T10457	cc	and,rescue
R2894	T10478	T10457	conj	induce,rescue
R2895	T10479	T10478	dobj	nephrogenesis,induce
R2896	T10566	T10567	mark	as,expected
R2897	T10480	T10479	prep	of,nephrogenesis
R2898	T10567	T10557	advcl	expected,undergo
R2899	T10568	T10557	punct	", ",undergo
R29	T973	T972	compound	β,catenin
R290	T1260	T1258	pobj	genes,of
R2900	T10481	T10482	det	the,mesenchyme
R2901	T10569	T10557	cc	but,undergo
R2902	T10570	T10557	conj	failed,undergo
R2903	T10482	T10480	pobj	mesenchyme,of
R2904	T10571	T10572	aux	to,show
R2905	T10572	T10570	xcomp	show,failed
R2906	T10573	T10574	compound	BAT,gal
R2907	T10483	T10482	amod	metanephric,mesenchyme
R2908	T10574	T10576	compound	gal,expression
R2909	T10575	T10574	punct	-,gal
R291	T1261	T1260	punct	", ",genes
R2910	T10576	T10572	dobj	expression,show
R2911	T10484	T10485	punct	[,2
R2912	T10485	T10478	parataxis	2,induce
R2913	T10577	T10578	punct	(,shown
R2914	T10578	T10572	parataxis	shown,show
R2915	T10486	T10485	punct	],2
R2916	T10579	T10578	nsubj	data,shown
R2917	T10580	T10578	neg	not,shown
R2918	T10581	T10578	punct	),shown
R2919	T10487	T10457	punct	.,rescue
R292	T1262	T1260	prep	including,genes
R2920	T10582	T10570	punct	", ",failed
R2921	T10583	T10570	advcl	suggesting,failed
R2922	T10584	T10585	mark	that,is
R2923	T10489	T10490	nsubj	This,suggests
R2924	T10585	T10583	ccomp	is,suggesting
R2925	T10586	T10587	det	this,transgene
R2926	T10587	T10585	nsubj	transgene,is
R2927	T10491	T10492	mark	that,report
R2928	T10588	T10585	neg	not,is
R2929	T10589	T10590	det	an,reporter
R293	T1263	T1262	pobj	Cxcl13,including
R2930	T10590	T10585	attr	reporter,is
R2931	T10591	T10590	amod	accurate,reporter
R2932	T10492	T10490	ccomp	report,suggests
R2933	T10592	T10590	prep	of,reporter
R2934	T10593	T10594	amod	canonical,signaling
R2935	T10594	T10592	pobj	signaling,of
R2936	T10493	T10494	det	the,transgene
R2937	T10595	T10594	compound	Wnt,signaling
R2938	T10596	T10590	prep	in,reporter
R2939	T10597	T10598	det	the,mesenchyme
R294	T1264	T1263	punct	", ",Cxcl13
R2940	T10494	T10492	nsubj	transgene,report
R2941	T10598	T10596	pobj	mesenchyme,in
R2942	T10599	T10598	amod	metanephric,mesenchyme
R2943	T10600	T10598	prep	of,mesenchyme
R2944	T10601	T10602	det	the,kidney
R2945	T10602	T10600	pobj	kidney,of
R2946	T10603	T10602	amod	developing,kidney
R2947	T10495	T10496	compound	BAT,gal
R2948	T10604	T10555	punct	.,observed
R2949	T10496	T10494	compound	gal,transgene
R295	T1265	T1263	conj	Slc5a2,Cxcl13
R2950	T10497	T10496	punct	-,gal
R2951	T10498	T10492	aux	might,report
R2952	T10499	T10492	neg	not,report
R2953	T10500	T10492	advmod	accurately,report
R2954	T10501	T10502	amod	canonical,signaling
R2955	T10502	T10492	dobj	signaling,report
R2956	T10503	T10502	compound	Wnt,signaling
R2957	T10504	T10492	prep	in,report
R2958	T10505	T10506	det	the,mesenchyme
R2959	T10506	T10504	pobj	mesenchyme,in
R296	T1266	T1265	punct	", ",Slc5a2
R2960	T10507	T10506	amod	metanephric,mesenchyme
R2961	T10508	T10490	punct	.,suggests
R2962	T10510	T10511	aux	To,address
R2963	T10511	T10512	advcl	address,incubated
R2964	T10513	T10514	det	this,question
R2965	T10514	T10511	dobj	question,address
R2966	T10515	T10512	punct	", ",incubated
R2967	T10516	T10512	nsubj	we,incubated
R2968	T10517	T10518	nmod	E11.5,mesenchyme
R2969	T10518	T10512	dobj	mesenchyme,incubated
R297	T1267	T1265	conj	Klk5,Slc5a2
R2970	T10519	T10518	amod	metanephric,mesenchyme
R2971	T10520	T10512	prep	in,incubated
R2972	T10521	T10522	compound	lithium,chloride
R2973	T10522	T10520	pobj	chloride,in
R2974	T10523	T10522	punct	(,chloride
R2975	T10524	T10522	appos	LiCl,chloride
R2976	T10525	T10522	punct	),chloride
R2977	T10526	T10522	punct	", ",chloride
R2978	T10527	T10528	dep	which,activates
R2979	T10528	T10522	relcl	activates,chloride
R298	T1268	T1267	punct	", ",Klk5
R2980	T10529	T10530	amod	canonical,signaling
R2981	T10530	T10528	dobj	signaling,activates
R2982	T10531	T10530	compound	Wnt,signaling
R2983	T10532	T10528	prep	through,activates
R2984	T10533	T10532	pobj	inhibition,through
R2985	T10534	T10533	prep	of,inhibition
R2986	T10535	T10534	pobj	GSK3,of
R2987	T10536	T10528	punct	", ",activates
R2988	T10537	T10528	cc	and,activates
R2989	T10538	T10539	advmod	also,functions
R299	T1269	T1267	conj	Ren2,Klk5
R2990	T10539	T10528	conj	functions,activates
R2991	T10540	T10539	prep	as,functions
R2992	T10541	T10542	det	an,inducer
R2993	T10542	T10540	pobj	inducer,as
R2994	T10543	T10542	prep	of,inducer
R2995	T10544	T10543	pobj	nephrogenesis,of
R2996	T10545	T10542	prep	in,inducer
R2997	T10546	T10547	compound	kidney,organ
R2998	T10547	T10548	compound	organ,culture
R2999	T10548	T10545	pobj	culture,in
R30	T974	T972	punct	-,catenin
R300	T1270	T1269	cc	and,Ren2
R3000	T10549	T10550	punct	[,22
R3001	T10550	T10539	parataxis	22,functions
R3002	T10551	T10550	punct	],22
R3003	T10552	T10512	punct	.,incubated
R3004	T10554	T10555	nsubj	We,observed
R3005	T10556	T10557	mark	that,undergo
R3006	T10557	T10555	ccomp	undergo,observed
R3007	T10558	T10559	npadvmod	LiCl,treated
R3008	T10559	T10561	amod	treated,mesenchyme
R3009	T10560	T10559	punct	-,treated
R301	T1271	T1269	conj	Timeless,Ren2
R3010	T10561	T10557	nsubj	mesenchyme,undergo
R3011	T10562	T10561	amod	metanephric,mesenchyme
R3012	T10563	T10557	aux	did,undergo
R3013	T10564	T10557	dobj	nephrogenesis,undergo
R3014	T10565	T10557	punct	", ",undergo
R3017	T12039	T12040	compound	Microarray,analysis
R3018	T12041	T12040	prep	of,analysis
R3019	T12042	T12043	compound	Pygo1,Pygo2
R302	T1272	T1260	punct	", ",genes
R3020	T12043	T12045	npadvmod	Pygo2,null
R3021	T12044	T12043	punct	/,Pygo2
R3022	T12045	T12046	amod	null,kidneys
R3023	T12046	T12041	pobj	kidneys,of
R3024	T12048	T12049	aux	To,examine
R3025	T12049	T12051	advcl	examine,used
R3026	T12050	T12049	advmod	further,examine
R3027	T12052	T12053	amod	possible,disturbance
R3028	T12053	T12049	dobj	disturbance,examine
R3029	T12054	T12053	prep	of,disturbance
R303	T1273	T1274	dep	which,represent
R3030	T12055	T12056	compound	gene,expression
R3031	T12056	T12054	pobj	expression,of
R3032	T12057	T12049	prep	in,examine
R3033	T12058	T12059	det	the,kidneys
R3034	T12059	T12057	pobj	kidneys,in
R3035	T12060	T12061	compound	Pygo1,Pygo2
R3036	T12061	T12059	compound	Pygo2,kidneys
R3037	T12062	T12061	punct	/,Pygo2
R3038	T12063	T12059	compound	mutant,kidneys
R3039	T12064	T12051	punct	", ",used
R304	T1274	T1260	relcl	represent,genes
R3040	T12065	T12051	nsubj	we,used
R3041	T12066	T12051	dobj	microarrays,used
R3042	T12067	T12068	aux	to,perform
R3043	T12068	T12051	advcl	perform,used
R3044	T12069	T12070	det	a,analysis
R3045	T12070	T12068	dobj	analysis,perform
R3046	T12071	T12070	amod	global,analysis
R3047	T12072	T12070	prep	of,analysis
R3048	T12073	T12074	compound	gene,expression
R3049	T12074	T12075	compound	expression,changes
R305	T1275	T1276	compound	candidate,targets
R3050	T12075	T12072	pobj	changes,of
R3051	T12076	T12051	punct	.,used
R3052	T12078	T12079	mark	Whereas,monitors
R3053	T12079	T12086	advcl	monitors,used
R3054	T12080	T12081	det	the,reporter
R3055	T12081	T12079	nsubj	reporter,monitors
R3056	T12082	T12083	compound	BAT,gal
R3057	T12083	T12085	compound	gal,transgene
R3058	T12084	T12083	punct	-,gal
R3059	T12085	T12081	compound	transgene,reporter
R306	T1276	T1274	dobj	targets,represent
R3060	T12087	T12088	det	the,response
R3061	T12088	T12079	dobj	response,monitors
R3062	T12089	T12088	prep	of,response
R3063	T12090	T12091	nummod	one,promoter
R3064	T12091	T12089	pobj	promoter,of
R3065	T12092	T12088	prep	to,response
R3066	T12093	T12094	compound	Wnt,signaling
R3067	T12094	T12092	pobj	signaling,to
R3068	T12095	T12086	punct	", ",used
R3069	T12096	T12086	nsubjpass	microarrays,used
R307	T1277	T1276	compound	Wnt,targets
R3070	T12097	T12086	aux	can,used
R3071	T12098	T12086	auxpass	be,used
R3072	T12099	T12100	aux	to,follow
R3073	T12100	T12086	advcl	follow,used
R3074	T12101	T12102	compound	expression,changes
R3075	T12102	T12100	dobj	changes,follow
R3076	T12103	T12102	prep	of,changes
R3077	T12104	T12105	det	all,genes
R3078	T12105	T12103	pobj	genes,of
R3079	T12106	T12105	punct	", ",genes
R308	T1278	T1276	prep	in,targets
R3080	T12107	T12105	prep	including,genes
R3081	T12108	T12109	det	all,targets
R3082	T12109	T12107	pobj	targets,including
R3083	T12110	T12109	amod	known,targets
R3084	T12111	T12109	compound	Wnt,targets
R3085	T12112	T12086	punct	.,used
R3086	T12114	T12115	nmod	E18.5,kidneys
R3087	T12115	T12129	nsubjpass	kidneys,examined
R3088	T12116	T12117	amod	wild,type
R3089	T12117	T12115	nmod	type,kidneys
R309	T1279	T1280	compound	kidney,development
R3090	T12118	T12117	punct	-,type
R3091	T12119	T12117	cc	and,type
R3092	T12120	T12117	conj	Pygo1,type
R3093	T12121	T12120	punct	-,Pygo1
R3094	T12122	T12120	punct	/,Pygo1
R3095	T12123	T12120	punct	-,Pygo1
R3096	T12124	T12120	punct	/,Pygo1
R3097	T12125	T12120	appos	Pygo2,Pygo1
R3098	T12126	T12125	punct	-,Pygo2
R3099	T12127	T12125	punct	/,Pygo2
R31	T975	T972	punct	),catenin
R310	T1280	T1278	pobj	development,in
R3100	T12128	T12125	punct	-,Pygo2
R3101	T12130	T12129	auxpass	were,examined
R3102	T12131	T12129	prep	in,examined
R3103	T12132	T12133	amod	biological,triplicate
R3104	T12133	T12131	pobj	triplicate,in
R3105	T12134	T12129	punct	.,examined
R3106	T12136	T12137	prep	In,identified
R3107	T12138	T12136	amod	total,In
R3108	T12139	T12137	punct	", ",identified
R3109	T12140	T12141	nummod	45,genes
R311	T1281	T1255	punct	.,showed
R3110	T12141	T12137	nsubjpass	genes,identified
R3111	T12142	T12137	auxpass	were,identified
R3112	T12143	T12137	prep	as,identified
R3113	T12144	T12145	advmod	significantly,changed
R3114	T12145	T12143	amod	changed,as
R3115	T12146	T12137	punct	", ",identified
R3116	T12147	T12137	advcl	using,identified
R3117	T12148	T12149	det	a,screen
R3118	T12149	T12147	dobj	screen,using
R3119	T12150	T12151	advmod	relatively,low
R312	T1285	T1286	det	The,genes
R3120	T12151	T12149	amod	low,screen
R3121	T12152	T12149	compound	stringency,screen
R3122	T12153	T12149	prep	of,screen
R3123	T12154	T12155	det	the,data
R3124	T12155	T12153	pobj	data,of
R3125	T12156	T12137	punct	", ",identified
R3126	T12157	T12137	prep	with,identified
R3127	T12158	T12159	det	a,cutoff
R3128	T12159	T12157	pobj	cutoff,with
R3129	T12160	T12161	compound	p,value
R313	T1286	T1289	nsubjpass	genes,required
R3130	T12161	T12159	compound	value,cutoff
R3131	T12162	T12161	punct	-,value
R3132	T12163	T12159	prep	of,cutoff
R3133	T12164	T12165	punct	<,0.05
R3134	T12165	T12163	pobj	0.05,of
R3135	T12166	T12159	punct	", ",cutoff
R3136	T12167	T12159	cc	and,cutoff
R3137	T12168	T12169	compound	fold,change
R3138	T12169	T12159	conj	change,cutoff
R3139	T12170	T12171	punct	>,2
R314	T1287	T1286	amod	mammalian,genes
R3140	T12171	T12169	appos	2,change
R3141	T12172	T12173	punct	(,Table
R3142	T12173	T12137	parataxis	Table,identified
R3143	T12174	T12173	nummod	3,Table
R3144	T12175	T12173	punct	),Table
R3145	T12176	T12137	punct	.,identified
R3146	T12178	T12179	advmod	Notably,identified
R3147	T12180	T12179	punct	", ",identified
R3148	T12181	T12182	preconj	both,Pygo1
R3149	T12182	T12179	nsubjpass	Pygo1,identified
R315	T1288	T1286	compound	Pygopus,genes
R3150	T12183	T12182	cc	and,Pygo1
R3151	T12184	T12182	conj	Pygo2,Pygo1
R3152	T12185	T12179	auxpass	were,identified
R3153	T12186	T12179	prep	as,identified
R3154	T12187	T12186	amod	downregulated,as
R3155	T12188	T12189	punct	(,respectively
R3156	T12189	T12187	parataxis	respectively,downregulated
R3157	T12190	T12191	punct	-,5.3-fold
R3158	T12191	T12189	advmod	5.3-fold,respectively
R3159	T12192	T12191	cc	and,5.3-fold
R316	T1290	T1289	auxpass	are,required
R3160	T12193	T12194	punct	-,3.9-fold
R3161	T12194	T12191	conj	3.9-fold,5.3-fold
R3162	T12195	T12189	punct	", ",respectively
R3163	T12196	T12189	punct	),respectively
R3164	T12197	T12179	prep	in,identified
R3165	T12198	T12199	det	the,kidneys
R3166	T12199	T12197	pobj	kidneys,in
R3167	T12200	T12199	compound	mutant,kidneys
R3168	T12201	T12179	punct	.,identified
R3169	T12203	T12204	amod	Other,genes
R317	T1291	T1289	prep	for,required
R3170	T12204	T12205	nsubj	genes,included
R3171	T12206	T12204	prep	with,genes
R3172	T12207	T12208	amod	significant,decreases
R3173	T12208	T12206	pobj	decreases,with
R3174	T12209	T12208	prep	in,decreases
R3175	T12210	T12211	compound	mutant,kidneys
R3176	T12211	T12209	pobj	kidneys,in
R3177	T12212	T12205	dobj	Cxcl13,included
R3178	T12213	T12214	punct	(,2.3-fold
R3179	T12214	T12212	parataxis	2.3-fold,Cxcl13
R318	T1292	T1293	amod	normal,morphogenesis
R3180	T12215	T12214	punct	-,2.3-fold
R3181	T12216	T12214	punct	),2.3-fold
R3182	T12217	T12212	punct	", ",Cxcl13
R3183	T12218	T12212	conj	Slc5a2,Cxcl13
R3184	T12219	T12220	punct	(,2.8-fold
R3185	T12220	T12218	parataxis	2.8-fold,Slc5a2
R3186	T12221	T12220	punct	-,2.8-fold
R3187	T12222	T12220	punct	),2.8-fold
R3188	T12223	T12218	punct	", ",Slc5a2
R3189	T12224	T12218	cc	and,Slc5a2
R319	T1293	T1291	pobj	morphogenesis,for
R3190	T12225	T12218	conj	Slco1a4,Slc5a2
R3191	T12226	T12227	punct	(,2.1-fold
R3192	T12227	T12225	parataxis	2.1-fold,Slco1a4
R3193	T12228	T12227	punct	-,2.1-fold
R3194	T12229	T12227	punct	),2.1-fold
R3195	T12230	T12205	punct	.,included
R3196	T12232	T12233	nsubjpass	Slc5a2,expressed
R3197	T12234	T12233	auxpass	is,expressed
R3198	T12235	T12233	prep	in,expressed
R3199	T12236	T12237	det	the,tubules
R32	T976	T970	compound	transcription,factor
R320	T1294	T1293	compound	branching,morphogenesis
R3200	T12237	T12235	pobj	tubules,in
R3201	T12238	T12237	amod	proximal,tubules
R3202	T12239	T12237	prep	of,tubules
R3203	T12240	T12241	det	the,nephron
R3204	T12241	T12239	pobj	nephron,of
R3205	T12242	T12241	amod	adult,nephron
R3206	T12243	T12233	cc	and,expressed
R3207	T12244	T12245	aux	has,implicated
R3208	T12245	T12233	conj	implicated,expressed
R3209	T12246	T12245	auxpass	been,implicated
R321	T1295	T1293	prep	of,morphogenesis
R3210	T12247	T12245	prep	in,implicated
R3211	T12248	T12249	amod	autosomal,recessive
R3212	T12249	T12250	amod	recessive,glucosuria
R3213	T12250	T12247	pobj	glucosuria,in
R3214	T12251	T12250	amod	renal,glucosuria
R3215	T12252	T12250	punct	", ",glucosuria
R3216	T12253	T12250	acl	characterized,glucosuria
R3217	T12254	T12253	agent	by,characterized
R3218	T12255	T12254	pobj	loss,by
R3219	T12256	T12255	prep	of,loss
R322	T1296	T1297	det	the,bud
R3220	T12257	T12258	compound	glucose,uptake
R3221	T12258	T12256	pobj	uptake,of
R3222	T12259	T12258	prep	by,uptake
R3223	T12260	T12261	det	the,nephron
R3224	T12261	T12259	pobj	nephron,by
R3225	T12262	T12263	punct	[,23
R3226	T12263	T12253	parataxis	23,characterized
R3227	T12264	T12263	punct	],23
R3228	T12265	T12233	punct	.,expressed
R3229	T12267	T12268	det	The,transporter
R323	T1297	T1295	pobj	bud,of
R3230	T12268	T12271	nsubjpass	transporter,expressed
R3231	T12269	T12268	amod	organic,transporter
R3232	T12270	T12268	compound	anion,transporter
R3233	T12272	T12268	punct	", ",transporter
R3234	T12273	T12268	appos	Slco1a4,transporter
R3235	T12274	T12271	punct	", ",expressed
R3236	T12275	T12271	auxpass	is,expressed
R3237	T12276	T12271	advmod	also,expressed
R3238	T12277	T12271	advmod	strongly,expressed
R3239	T12278	T12271	prep	in,expressed
R324	T1298	T1297	amod	ureteric,bud
R3240	T12279	T12280	det	the,tubules
R3241	T12280	T12278	pobj	tubules,in
R3242	T12281	T12280	prep	of,tubules
R3243	T12282	T12283	det	the,kidney
R3244	T12283	T12281	pobj	kidney,of
R3245	T12284	T12283	amod	adult,kidney
R3246	T12285	T12286	punct	[,24
R3247	T12286	T12271	parataxis	24,expressed
R3248	T12287	T12286	punct	],24
R3249	T12288	T12271	punct	.,expressed
R325	T1299	T1293	prep	during,morphogenesis
R3250	T12290	T12291	det	These,results
R3251	T12291	T12292	nsubj	results,suggest
R3252	T12293	T12294	mark	that,play
R3253	T12294	T12292	ccomp	play,suggest
R3254	T12295	T12296	det	the,genes
R3255	T12296	T12294	nsubj	genes,play
R3256	T12297	T12298	compound	Pygo1,Pygo2
R3257	T12298	T12296	compound	Pygo2,genes
R3258	T12299	T12298	punct	/,Pygo2
R3259	T12300	T12294	aux	might,play
R326	T1300	T1301	compound	kidney,development
R3260	T12301	T12302	det	a,role
R3261	T12302	T12294	dobj	role,play
R3262	T12303	T12294	prep	in,play
R3263	T12304	T12305	compound	nephron,maturation
R3264	T12305	T12303	pobj	maturation,in
R3265	T12306	T12305	cc	and,maturation
R3266	T12307	T12308	amod	subsequent,function
R3267	T12308	T12305	conj	function,maturation
R3268	T12309	T12292	punct	.,suggest
R3269	T12312	T12313	amod	Noteworthy,genes
R327	T1301	T1299	pobj	development,during
R3270	T12313	T12314	nsubj	genes,included
R3271	T12315	T12313	acl	upregulated,genes
R3272	T12316	T12315	prep	in,upregulated
R3273	T12317	T12318	det	the,kidney
R3274	T12318	T12316	pobj	kidney,in
R3275	T12319	T12320	compound	Pygo1,Pygo2
R3276	T12320	T12322	npadvmod	Pygo2,null
R3277	T12321	T12320	punct	/,Pygo2
R3278	T12322	T12318	amod	null,kidney
R3279	T12323	T12314	dobj	Klk5,included
R328	T1302	T1289	punct	.,required
R3280	T12324	T12325	punct	(,2.8-fold
R3281	T12325	T12323	parataxis	2.8-fold,Klk5
R3282	T12326	T12325	punct	),2.8-fold
R3283	T12327	T12323	punct	", ",Klk5
R3284	T12328	T12323	conj	Klk6,Klk5
R3285	T12329	T12330	punct	(,2.7-fold
R3286	T12330	T12328	parataxis	2.7-fold,Klk6
R3287	T12331	T12330	punct	),2.7-fold
R3288	T12332	T12328	punct	", ",Klk6
R3289	T12333	T12328	conj	Ren2,Klk6
R329	T1304	T1305	advmod	Nevertheless,indicates
R3290	T12334	T12335	punct	(,2.2-fold
R3291	T12335	T12333	parataxis	2.2-fold,Ren2
R3292	T12336	T12335	punct	),2.2-fold
R3293	T12337	T12333	punct	", ",Ren2
R3294	T12338	T12333	cc	and,Ren2
R3295	T12339	T12333	conj	Timeless,Ren2
R3296	T12340	T12341	punct	(,2.4-fold
R3297	T12341	T12339	parataxis	2.4-fold,Timeless
R3298	T12342	T12341	punct	),2.4-fold
R3299	T12343	T12314	punct	.,included
R33	T977	T968	prep	of,complex
R330	T1306	T1305	punct	", ",indicates
R3300	T12345	T12346	nsubj	Klk5,are
R3301	T12347	T12345	cc	and,Klk5
R3302	T12348	T12345	conj	Klk6,Klk5
R3303	T12349	T12346	attr	members,are
R3304	T12350	T12349	prep	of,members
R3305	T12351	T12352	det	the,family
R3306	T12352	T12350	pobj	family,of
R3307	T12353	T12352	compound	kallikrein,family
R3308	T12354	T12352	prep	of,family
R3309	T12355	T12356	npadvmod	trypsin,like
R331	T1307	T1308	det	the,phenotype
R3310	T12356	T12358	amod	like,proteases
R3311	T12357	T12356	punct	-,like
R3312	T12358	T12354	pobj	proteases,of
R3313	T12359	T12358	compound	serine,proteases
R3314	T12360	T12346	punct	.,are
R3315	T12362	T12363	nsubj	Kallikreins,have
R3316	T12364	T12365	amod	diverse,functions
R3317	T12365	T12363	dobj	functions,have
R3318	T12366	T12363	prep	in,have
R3319	T12367	T12366	pobj	cancer,in
R332	T1308	T1305	nsubj	phenotype,indicates
R3320	T12368	T12367	punct	", ",cancer
R3321	T12369	T12370	compound	tissue,remodeling
R3322	T12370	T12367	conj	remodeling,cancer
R3323	T12371	T12370	punct	", ",remodeling
R3324	T12372	T12370	cc	and,remodeling
R3325	T12373	T12370	conj	regulation,remodeling
R3326	T12374	T12373	prep	of,regulation
R3327	T12375	T12376	compound	blood,pressure
R3328	T12376	T12374	pobj	pressure,of
R3329	T12377	T12378	punct	[,25
R333	T1309	T1310	advmod	relatively,mild
R3330	T12378	T12363	parataxis	25,have
R3331	T12379	T12378	punct	],25
R3332	T12380	T12363	punct	.,have
R3333	T12382	T12383	nsubj	Ren2,activates
R3334	T12384	T12382	punct	", ",Ren2
R3335	T12385	T12386	det	a,homolog
R3336	T12386	T12382	appos	homolog,Ren2
R3337	T12387	T12386	prep	of,homolog
R3338	T12388	T12389	det	the,endopeptidase
R3339	T12389	T12387	pobj	endopeptidase,of
R334	T1310	T1308	amod	mild,phenotype
R3340	T12390	T12389	appos	Renin,endopeptidase
R3341	T12391	T12390	nummod	1,Renin
R3342	T12392	T12383	punct	", ",activates
R3343	T12393	T12394	det	the,system
R3344	T12394	T12383	dobj	system,activates
R3345	T12395	T12396	compound	renin,angiotensin
R3346	T12396	T12394	compound	angiotensin,system
R3347	T12397	T12396	punct	-,angiotensin
R3348	T12398	T12383	punct	", ",activates
R3349	T12399	T12383	advcl	increasing,activates
R335	T1311	T1308	acl	observed,phenotype
R3350	T12400	T12401	compound	blood,pressure
R3351	T12401	T12399	dobj	pressure,increasing
R3352	T12402	T12403	punct	[,26
R3353	T12403	T12383	parataxis	26,activates
R3354	T12404	T12403	punct	],26
R3355	T12405	T12383	punct	.,activates
R3356	T12407	T12408	nsubj	Disruption,results
R3357	T12409	T12407	prep	of,Disruption
R3358	T12410	T12411	det	the,system
R3359	T12411	T12409	pobj	system,of
R336	T1312	T1311	prep	in,observed
R3360	T12412	T12413	compound	renin,angiotensin
R3361	T12413	T12411	compound	angiotensin,system
R3362	T12414	T12413	punct	-,angiotensin
R3363	T12415	T12407	prep	during,Disruption
R3364	T12416	T12415	pobj	development,during
R3365	T12417	T12408	prep	in,results
R3366	T12418	T12419	amod	congenital,abnormalities
R3367	T12419	T12417	pobj	abnormalities,in
R3368	T12420	T12419	prep	of,abnormalities
R3369	T12421	T12422	det	the,ureter
R337	T1313	T1314	det	the,kidney
R3370	T12422	T12420	pobj	ureter,of
R3371	T12423	T12422	cc	and,ureter
R3372	T12424	T12425	compound	collecting,duct
R3373	T12425	T12427	compound	duct,system
R3374	T12426	T12425	punct	-,duct
R3375	T12427	T12422	conj	system,ureter
R3376	T12428	T12429	punct	[,27
R3377	T12429	T12408	parataxis	27,results
R3378	T12430	T12429	punct	],27
R3379	T12431	T12408	punct	.,results
R338	T1314	T1312	pobj	kidney,in
R3380	T12433	T12434	nsubjpass	Timeless,shown
R3381	T12435	T12433	punct	", ",Timeless
R3382	T12436	T12437	det	a,factor
R3383	T12437	T12433	appos	factor,Timeless
R3384	T12438	T12437	compound	transcription,factor
R3385	T12439	T12437	acl	involved,factor
R3386	T12440	T12439	prep	in,involved
R3387	T12441	T12442	det	the,regulation
R3388	T12442	T12440	pobj	regulation,in
R3389	T12443	T12442	prep	of,regulation
R339	T1315	T1314	punct	", ",kidney
R3390	T12444	T12445	amod	circadian,rhythms
R3391	T12445	T12443	pobj	rhythms,of
R3392	T12446	T12439	punct	", ",involved
R3393	T12447	T12439	cc	and,involved
R3394	T12448	T12439	conj	expressed,involved
R3395	T12449	T12448	prep	in,expressed
R3396	T12450	T12451	det	the,tips
R3397	T12451	T12449	pobj	tips,in
R3398	T12452	T12451	amod	branching,tips
R3399	T12453	T12451	amod	ureteric,tips
R34	T978	T979	amod	canonical,signaling
R340	T1316	T1317	advmod	as,as
R3400	T12454	T12434	punct	", ",shown
R3401	T12455	T12434	aux	has,shown
R3402	T12456	T12434	advmod	also,shown
R3403	T12457	T12434	auxpass	been,shown
R3404	T12458	T12459	aux	to,regulate
R3405	T12459	T12434	xcomp	regulate,shown
R3406	T12460	T12461	amod	ureteric,morphogenesis
R3407	T12461	T12459	dobj	morphogenesis,regulate
R3408	T12462	T12461	compound	branching,morphogenesis
R3409	T12463	T12464	punct	[,28
R341	T1317	T1314	cc	as,kidney
R3410	T12464	T12434	parataxis	28,shown
R3411	T12465	T12464	punct	],28
R3412	T12466	T12434	punct	.,shown
R3413	T12468	T12469	nsubj	We,were
R3414	T12470	T12469	acomp	interested,were
R3415	T12471	T12470	prep	in,interested
R3416	T12472	T12473	det	the,expression
R3417	T12473	T12471	pobj	expression,in
R3418	T12474	T12473	amod	possible,expression
R3419	T12475	T12473	amod	altered,expression
R342	T1318	T1317	advmod	well,as
R3420	T12476	T12473	prep	of,expression
R3421	T12477	T12478	advmod	previously,known
R3422	T12478	T12479	amod	known,targets
R3423	T12479	T12476	pobj	targets,of
R3424	T12480	T12479	compound	Wnt,targets
R3425	T12481	T12469	prep	in,were
R3426	T12482	T12483	det	the,kidneys
R3427	T12483	T12481	pobj	kidneys,in
R3428	T12484	T12485	nmod	Pygo1,mutant
R3429	T12485	T12483	compound	mutant,kidneys
R343	T1319	T1320	amod	other,systems
R3430	T12486	T12484	punct	-,Pygo1
R3431	T12487	T12484	punct	/,Pygo1
R3432	T12488	T12484	punct	-,Pygo1
R3433	T12489	T12484	punct	/,Pygo1
R3434	T12490	T12484	appos	Pygo2,Pygo1
R3435	T12491	T12490	punct	-,Pygo2
R3436	T12492	T12490	punct	/,Pygo2
R3437	T12493	T12490	punct	-,Pygo2
R3438	T12494	T12469	punct	.,were
R3439	T12496	T12497	det	A,list
R344	T1320	T1314	conj	systems,kidney
R3440	T12497	T12498	nsubjpass	list,compiled
R3441	T12499	T12497	prep	of,list
R3442	T12500	T12501	amod	known,targets
R3443	T12501	T12499	pobj	targets,of
R3444	T12502	T12501	compound	Wnt,targets
R3445	T12503	T12498	auxpass	was,compiled
R3446	T12504	T12498	prep	from,compiled
R3447	T12505	T12498	punct	.,compiled
R3448	T12507	T12508	preconj	Both,Pygo1
R3449	T12508	T12509	nmod	Pygo1,sets
R345	T1321	T1320	compound	organ,systems
R3450	T12509	T12513	nsubjpass	sets,included
R3451	T12510	T12508	cc	and,Pygo1
R3452	T12511	T12508	conj	Pygo2,Pygo1
R3453	T12512	T12509	compound	probe,sets
R3454	T12514	T12513	auxpass	were,included
R3455	T12515	T12513	prep	as,included
R3456	T12516	T12515	pobj	references,as
R3457	T12517	T12518	aux	to,illustrate
R3458	T12518	T12513	advcl	illustrate,included
R3459	T12519	T12520	amod	significant,regulation
R346	T1322	T1305	punct	", ",indicates
R3460	T12520	T12518	dobj	regulation,illustrate
R3461	T12521	T12520	amod	down,regulation
R3462	T12522	T12513	punct	.,included
R3463	T12524	T12525	advmod	Remarkably,showed
R3464	T12526	T12525	punct	", ",showed
R3465	T12527	T12528	det	the,genes
R3466	T12528	T12525	nsubj	genes,showed
R3467	T12529	T12528	amod	known,genes
R3468	T12530	T12531	compound	Wnt,target
R3469	T12531	T12528	compound	target,genes
R347	T1323	T1324	det	a,divergence
R3470	T12532	T12533	advmod	very,few
R3471	T12533	T12534	amod	few,differences
R3472	T12534	T12525	dobj	differences,showed
R3473	T12535	T12534	prep	in,differences
R3474	T12536	T12535	pobj	expression,in
R3475	T12537	T12534	prep	between,differences
R3476	T12538	T12539	amod	wild,type
R3477	T12539	T12541	nmod	type,kidneys
R3478	T12540	T12539	punct	-,type
R3479	T12541	T12537	pobj	kidneys,between
R348	T1324	T1305	dobj	divergence,indicates
R3480	T12542	T12539	cc	and,type
R3481	T12543	T12539	conj	mutant,type
R3482	T12544	T12545	punct	(,Figure
R3483	T12545	T12525	parataxis	Figure,showed
R3484	T12546	T12545	nummod	8,Figure
R3485	T12547	T12545	punct	),Figure
R3486	T12548	T12525	punct	.,showed
R3487	T12550	T12551	advmod	Only,expression
R3488	T12551	T12553	nsubjpass	expression,changed
R3489	T12552	T12551	det	the,expression
R349	T1325	T1324	amod	striking,divergence
R3490	T12554	T12551	prep	of,expression
R3491	T12555	T12556	npadvmod	Ccnd1,encoding
R3492	T12556	T12557	amod	encoding,D1
R3493	T12557	T12554	pobj	D1,of
R3494	T12558	T12557	compound	cyclin,D1
R3495	T12559	T12560	punct	[,29
R3496	T12560	T12557	parataxis	29,D1
R3497	T12561	T12560	punct	],29
R3498	T12562	T12557	punct	", ",D1
R3499	T12563	T12557	cc	and,D1
R35	T979	T977	pobj	signaling,of
R350	T1326	T1324	amod	evolutionary,divergence
R3500	T12564	T12557	conj	Wisp1,D1
R3501	T12565	T12566	punct	[,30
R3502	T12566	T12564	parataxis	30,Wisp1
R3503	T12567	T12566	punct	],30
R3504	T12568	T12553	auxpass	were,changed
R3505	T12569	T12553	advmod	significantly,changed
R3506	T12570	T12553	punct	", ",changed
R3507	T12571	T12553	prep	with,changed
R3508	T12572	T12573	compound	expression,level
R3509	T12573	T12574	compound	level,changes
R351	T1327	T1324	prep	of,divergence
R3510	T12574	T12571	pobj	changes,with
R3511	T12575	T12574	prep	of,changes
R3512	T12576	T12577	punct	<,1.5-fold
R3513	T12577	T12575	pobj	1.5-fold,of
R3514	T12578	T12574	prep	in,changes
R3515	T12579	T12580	compound	Pygo1,Pygo2
R3516	T12580	T12582	npadvmod	Pygo2,null
R3517	T12581	T12580	punct	/,Pygo2
R3518	T12582	T12583	amod	null,kidneys
R3519	T12583	T12578	pobj	kidneys,in
R352	T1328	T1329	compound	Pygopus,function
R3520	T12584	T12553	punct	.,changed
R3521	T12586	T12587	det	These,results
R3522	T12587	T12588	nsubj	results,indicate
R3523	T12589	T12590	det	an,absence
R3524	T12590	T12588	dobj	absence,indicate
R3525	T12591	T12590	prep	of,absence
R3526	T12592	T12593	amod	dramatic,changes
R3527	T12593	T12591	pobj	changes,of
R3528	T12594	T12593	prep	in,changes
R3529	T12595	T12594	pobj	expression,in
R353	T1329	T1327	pobj	function,of
R3530	T12596	T12595	prep	of,expression
R3531	T12597	T12598	advmod	previously,known
R3532	T12598	T12599	amod	known,genes
R3533	T12599	T12596	pobj	genes,of
R3534	T12600	T12601	compound	Wnt,signaling
R3535	T12601	T12599	compound	signaling,genes
R3536	T12602	T12599	compound	target,genes
R3537	T12603	T12595	prep	in,expression
R3538	T12604	T12605	det	the,kidneys
R3539	T12605	T12603	pobj	kidneys,in
R354	T1330	T1324	prep	between,divergence
R3540	T12606	T12607	compound	Pygo1,Pygo2
R3541	T12607	T12609	compound	Pygo2,mutant
R3542	T12608	T12607	punct	/,Pygo2
R3543	T12609	T12605	compound	mutant,kidneys
R3544	T12610	T12588	punct	.,indicate
R3545	T12612	T12613	nsubj	We,used
R3546	T12614	T12615	amod	quantitative,PCR
R3547	T12615	T12613	dobj	PCR,used
R3548	T12616	T12617	amod	real,time
R3549	T12617	T12615	compound	time,PCR
R355	T1331	T1330	pobj	mammals,between
R3550	T12618	T12617	punct	-,time
R3551	T12619	T12615	punct	", ",PCR
R3552	T12620	T12615	prep	with,PCR
R3553	T12621	T12622	amod	independent,samples
R3554	T12622	T12620	pobj	samples,with
R3555	T12623	T12622	amod	biological,samples
R3556	T12624	T12613	punct	", ",used
R3557	T12625	T12626	aux	to,validate
R3558	T12626	T12613	advcl	validate,used
R3559	T12627	T12628	det	the,results
R356	T1332	T1331	cc	and,mammals
R3560	T12628	T12626	dobj	results,validate
R3561	T12629	T12628	compound	microarray,results
R3562	T12630	T12613	punct	.,used
R3563	T12632	T12633	nummod	Nine,genes
R3564	T12633	T12634	nsubjpass	genes,tested
R3565	T12635	T12634	auxpass	were,tested
R3566	T12636	T12634	punct	", ",tested
R3567	T12637	T12634	cc	and,tested
R3568	T12638	T12639	prep	for,observe
R3569	T12639	T12634	conj	observe,tested
R357	T1333	T1331	conj	Drosophila,mammals
R3570	T12640	T12638	pobj	seven,for
R3571	T12641	T12639	nsubj	we,observe
R3572	T12642	T12639	aux	did,observe
R3573	T12643	T12644	det	a,change
R3574	T12644	T12639	dobj	change,observe
R3575	T12645	T12644	amod	significant,change
R3576	T12646	T12644	compound	fold,change
R3577	T12647	T12644	prep	in,change
R3578	T12648	T12649	det	the,direction
R3579	T12649	T12647	pobj	direction,in
R358	T1334	T1305	punct	.,indicates
R3580	T12650	T12649	amod	same,direction
R3581	T12651	T12649	acl	predicted,direction
R3582	T12652	T12651	agent	by,predicted
R3583	T12653	T12654	det	the,results
R3584	T12654	T12652	pobj	results,by
R3585	T12655	T12654	compound	microarray,results
R3586	T12656	T12657	punct	(,Table
R3587	T12657	T12639	parataxis	Table,observe
R3588	T12658	T12657	nummod	4,Table
R3589	T12659	T12657	punct	),Table
R359	T1336	T1337	prep	In,required
R3590	T12660	T12639	punct	.,observe
R3591	T12662	T12663	nsubj	It,is
R3592	T12664	T12665	neg	not,surprising
R3593	T12665	T12663	acomp	surprising,is
R3594	T12666	T12667	mark	that,validated
R3595	T12667	T12663	ccomp	validated,is
R3596	T12668	T12667	nsubjpass	two,validated
R3597	T12669	T12668	prep	of,two
R3598	T12670	T12671	det	the,genes
R3599	T12671	T12669	pobj	genes,of
R36	T980	T979	compound	Wnt,signaling
R360	T1338	T1336	pobj	mammals,In
R3600	T12672	T12671	nummod	nine,genes
R3601	T12673	T12667	aux	could,validated
R3602	T12674	T12667	neg	not,validated
R3603	T12675	T12667	auxpass	be,validated
R3604	T12676	T12663	punct	", ",is
R3605	T12677	T12663	advcl	considering,is
R3606	T12678	T12679	det	the,stringency
R3607	T12679	T12677	dobj	stringency,considering
R3608	T12680	T12681	advmod	relatively,low
R3609	T12681	T12679	amod	low,stringency
R361	T1339	T1337	punct	", ",required
R3610	T12682	T12679	acl	used,stringency
R3611	T12683	T12682	prep	in,used
R3612	T12684	T12683	pcomp	screening,in
R3613	T12685	T12686	det	the,data
R3614	T12686	T12684	dobj	data,screening
R3615	T12687	T12686	compound	microarray,data
R3616	T12688	T12663	punct	.,is
R362	T1340	T1341	det	the,genes
R3623	T15289	T15290	prep	In,is
R3624	T15291	T15289	pobj	Drosophila,In
R3625	T15292	T15290	punct	", ",is
R3626	T15293	T15294	det	the,gene
R3627	T15294	T15290	nsubj	gene,is
R3628	T15295	T15294	compound	Pygopus,gene
R3629	T15296	T15297	det	a,mediator
R363	T1341	T1337	nsubjpass	genes,required
R3630	T15297	T15290	attr	mediator,is
R3631	T15298	T15297	amod	key,mediator
R3632	T15299	T15297	prep	of,mediator
R3633	T15300	T15301	amod	canonical,signaling
R3634	T15301	T15299	pobj	signaling,of
R3635	T15302	T15301	compound	Wnt,signaling
R3636	T15303	T15290	punct	.,is
R3637	T15305	T15306	prep	In,performed
R3638	T15307	T15308	nummod	one,study
R3639	T15308	T15305	pobj	study,In
R364	T1342	T1343	compound	Pygo1,Pygo2
R3640	T15309	T15306	punct	", ",performed
R3641	T15310	T15311	nummod	12,measures
R3642	T15311	T15306	nsubjpass	measures,performed
R3643	T15312	T15311	amod	distinct,measures
R3644	T15313	T15311	prep	of,measures
R3645	T15314	T15315	compound	Wnt,signaling
R3646	T15315	T15313	pobj	signaling,of
R3647	T15316	T15315	prep	in,signaling
R3648	T15317	T15318	compound	Pygo,mutants
R3649	T15318	T15316	pobj	mutants,in
R365	T1343	T1341	compound	Pygo2,genes
R3650	T15319	T15306	auxpass	were,performed
R3651	T15320	T15306	punct	", ",performed
R3652	T15321	T15306	prep	including,performed
R3653	T15322	T15321	pobj	analysis,including
R3654	T15323	T15322	prep	of,analysis
R3655	T15324	T15325	npadvmod	leg,imaginal
R3656	T15325	T15331	amod	imaginal,discs
R3657	T15326	T15324	punct	", ",leg
R3658	T15327	T15324	conj	wing,leg
R3659	T15328	T15327	punct	", ",wing
R366	T1344	T1343	punct	/,Pygo2
R3660	T15329	T15327	cc	and,wing
R3661	T15330	T15327	conj	eye,wing
R3662	T15331	T15323	pobj	discs,of
R3663	T15332	T15306	punct	.,performed
R3664	T15334	T15335	prep	In,was
R3665	T15336	T15337	nummod	2,cases
R3666	T15337	T15334	pobj	cases,In
R3667	T15338	T15335	expl	there,was
R3668	T15339	T15340	det	a,reduction
R3669	T15340	T15335	attr	reduction,was
R367	T1345	T1337	auxpass	are,required
R3670	T15341	T15340	amod	significant,reduction
R3671	T15342	T15340	prep	of,reduction
R3672	T15343	T15344	compound	Wnt,signaling
R3673	T15344	T15342	pobj	signaling,of
R3674	T15345	T15335	cc	and,was
R3675	T15346	T15347	prep	in,block
R3676	T15347	T15335	conj	block,was
R3677	T15348	T15349	nummod	10,cases
R3678	T15349	T15346	pobj	cases,in
R3679	T15350	T15347	det	a,block
R368	T1346	T1337	neg	not,required
R3680	T15351	T15347	amod	complete,block
R3681	T15352	T15353	punct	[,10
R3682	T15353	T15347	parataxis	10,block
R3683	T15354	T15353	punct	],10
R3684	T15355	T15347	punct	.,block
R3685	T15357	T15358	det	A,study
R3686	T15358	T15360	nsubj	study,examined
R3687	T15359	T15358	amod	second,study
R3688	T15361	T15358	prep	in,study
R3689	T15362	T15361	pobj	Drosophila,in
R369	T1347	T1337	advmod	absolutely,required
R3690	T15363	T15364	det	the,effects
R3691	T15364	T15360	dobj	effects,examined
R3692	T15365	T15364	prep	of,effects
R3693	T15366	T15367	compound	Pygo,mutation
R3694	T15367	T15365	pobj	mutation,of
R3695	T15368	T15364	prep	on,effects
R3696	T15369	T15370	compound	cuticle,patterning
R3697	T15370	T15368	pobj	patterning,on
R3698	T15371	T15370	punct	", ",patterning
R3699	T15372	T15373	compound	midgut,constriction
R37	T981	T960	punct	.,encodes
R370	T1348	T1337	prep	for,required
R3700	T15373	T15370	conj	constriction,patterning
R3701	T15374	T15373	punct	", ",constriction
R3702	T15375	T15376	amod	central,system
R3703	T15376	T15373	conj	system,constriction
R3704	T15377	T15376	amod	nervous,system
R3705	T15378	T15376	punct	", ",system
R3706	T15379	T15376	cc	and,system
R3707	T15380	T15381	amod	cardiac,development
R3708	T15381	T15376	conj	development,system
R3709	T15382	T15360	punct	", ",examined
R371	T1349	T1350	amod	canonical,signaling
R3710	T15383	T15360	cc	and,examined
R3711	T15384	T15360	conj	concluded,examined
R3712	T15385	T15386	mark	that,is
R3713	T15386	T15384	ccomp	is,concluded
R3714	T15387	T15386	punct	"""",is
R3715	T15388	T15386	nsubj	Pygo,is
R3716	T15389	T15390	det	an,component
R3717	T15390	T15386	attr	component,is
R3718	T15391	T15390	amod	essential,component
R3719	T15392	T15390	prep	in,component
R372	T1350	T1348	pobj	signaling,for
R3720	T15393	T15394	det	the,pathway
R3721	T15394	T15392	pobj	pathway,in
R3722	T15395	T15394	compound	Wg,pathway
R3723	T15396	T15397	compound	signal,transduction
R3724	T15397	T15394	compound	transduction,pathway
R3725	T15398	T15384	punct	"""",concluded
R3726	T15399	T15384	punct	.,concluded
R3727	T15400	T15401	punct	[,8
R3728	T15401	T15384	parataxis	8,concluded
R3729	T15402	T15401	punct	],8
R373	T1351	T1350	compound	Wnt,signaling
R3730	T15403	T15360	punct	.,examined
R3731	T15405	T15406	prep	Given,were
R3732	T15407	T15408	det	these,results
R3733	T15408	T15405	pobj	results,Given
R3734	T15409	T15408	prep	in,results
R3735	T15410	T15409	pobj	Drosophila,in
R3736	T15411	T15406	punct	", ",were
R3737	T15412	T15413	det	the,phenotypes
R3738	T15413	T15406	nsubj	phenotypes,were
R3739	T15414	T15415	advmod	relatively,mild
R374	T1352	T1337	prep	in,required
R3740	T15415	T15413	amod	mild,phenotypes
R3741	T15416	T15413	prep	of,phenotypes
R3742	T15417	T15416	pobj	mice,of
R3743	T15418	T15417	prep	with,mice
R3744	T15419	T15420	amod	targeted,Pygo1
R3745	T15420	T15421	nmod	Pygo1,mutations
R3746	T15421	T15418	pobj	mutations,with
R3747	T15422	T15420	punct	", ",Pygo1
R3748	T15423	T15420	conj	Pygo2,Pygo1
R3749	T15424	T15423	punct	", ",Pygo2
R375	T1353	T1354	amod	most,systems
R3750	T15425	T15423	cc	or,Pygo2
R3751	T15426	T15427	amod	double,Pygo2
R3752	T15427	T15423	conj	Pygo2,Pygo2
R3753	T15428	T15427	compound	Pygo1,Pygo2
R3754	T15429	T15427	punct	/,Pygo2
R3755	T15430	T15406	acomp	striking,were
R3756	T15431	T15406	punct	.,were
R3757	T15433	T15434	compound	Pygo2,mutants
R3758	T15434	T15435	nsubj	mutants,developed
R3759	T15436	T15435	prep	to,developed
R376	T1354	T1352	pobj	systems,in
R3760	T15437	T15436	pobj	birth,to
R3761	T15438	T15435	cc	and,developed
R3762	T15439	T15435	conj	showed,developed
R3763	T15440	T15441	amod	limited,abnormalities
R3764	T15441	T15439	dobj	abnormalities,showed
R3765	T15442	T15439	punct	", ",showed
R3766	T15443	T15444	mark	while,were
R3767	T15444	T15439	advcl	were,showed
R3768	T15445	T15446	npadvmod	Pygo1,homozygous
R3769	T15446	T15447	amod	homozygous,mutants
R377	T1355	T1354	amod	developing,systems
R3770	T15447	T15444	nsubj	mutants,were
R3771	T15448	T15444	acomp	normal,were
R3772	T15449	T15448	cc	and,normal
R3773	T15450	T15448	conj	fertile,normal
R3774	T15451	T15435	punct	.,developed
R3775	T15453	T15454	advmod	Furthermore,was
R3776	T15455	T15454	punct	", ",was
R3777	T15456	T15454	expl	there,was
R3778	T15457	T15458	det	no,synergism
R3779	T15458	T15454	attr	synergism,was
R378	T1356	T1337	punct	", ",required
R3780	T15459	T15458	amod	detected,synergism
R3781	T15460	T15458	prep	in,synergism
R3782	T15461	T15462	det	the,phenotype
R3783	T15462	T15460	pobj	phenotype,in
R3784	T15463	T15462	prep	of,phenotype
R3785	T15464	T15465	det	the,mutant
R3786	T15465	T15463	pobj	mutant,of
R3787	T15466	T15465	amod	double,mutant
R3788	T15467	T15468	compound	Pygo1,Pygo2
R3789	T15468	T15465	compound	Pygo2,mutant
R379	T1357	T1337	cc	but,required
R3790	T15469	T15468	punct	/,Pygo2
R3791	T15470	T15454	punct	", ",was
R3792	T15471	T15472	mark	although,show
R3793	T15472	T15454	advcl	show,was
R3794	T15473	T15472	prep	in,show
R3795	T15474	T15475	amod	several,tissues
R3796	T15475	T15473	pobj	tissues,in
R3797	T15476	T15477	det	the,reporter
R3798	T15477	T15472	nsubj	reporter,show
R3799	T15478	T15479	compound	BAT,gal
R38	T983	T984	aux	To,understand
R380	T1358	T1357	advmod	rather,but
R3800	T15479	T15477	compound	gal,reporter
R3801	T15480	T15479	punct	-,gal
R3802	T15481	T15477	compound	Wnt,reporter
R3803	T15482	T15472	aux	did,show
R3804	T15483	T15472	advmod	more,show
R3805	T15484	T15485	det	a,reduction
R3806	T15485	T15472	dobj	reduction,show
R3807	T15486	T15485	amod	severe,reduction
R3808	T15487	T15485	prep	in,reduction
R3809	T15488	T15487	pobj	expression,in
R381	T1359	T1337	conj	function,required
R3810	T15489	T15454	punct	.,was
R3811	T15491	T15492	nummod	One,explanation
R3812	T15492	T15494	nsubj	explanation,is
R3813	T15493	T15492	amod	possible,explanation
R3814	T15495	T15492	prep	of,explanation
R3815	T15496	T15497	det	these,results
R3816	T15497	T15495	pobj	results,of
R3817	T15498	T15497	amod	unexpected,results
R3818	T15499	T15500	det	a,failure
R3819	T15500	T15494	attr	failure,is
R382	T1360	T1359	prep	as,function
R3820	T15501	T15500	prep	of,failure
R3821	T15502	T15503	det	the,gene
R3822	T15503	T15501	pobj	gene,of
R3823	T15504	T15503	amod	targeting,gene
R3824	T15505	T15506	aux	to,eliminate
R3825	T15506	T15500	acl	eliminate,failure
R3826	T15507	T15506	dobj	functioning,eliminate
R3827	T15508	T15507	prep	of,functioning
R3828	T15509	T15510	det	the,genes
R3829	T15510	T15508	pobj	genes,of
R383	T1361	T1362	amod	quantitative,transducers
R3830	T15511	T15510	nmod	Pygo1,genes
R3831	T15512	T15511	cc	and,Pygo1
R3832	T15513	T15511	conj	Pygo2,Pygo1
R3833	T15514	T15494	punct	.,is
R3834	T15516	T15517	det	The,alleles
R3835	T15517	T15520	nsubj	alleles,are
R3836	T15518	T15519	compound	Pygopus,deletion
R3837	T15519	T15517	compound	deletion,alleles
R3838	T15521	T15517	acl	described,alleles
R3839	T15522	T15521	prep	in,described
R384	T1362	T1360	pobj	transducers,as
R3840	T15523	T15524	det	this,report
R3841	T15524	T15522	pobj	report,in
R3842	T15525	T15520	punct	", ",are
R3843	T15526	T15520	advmod	however,are
R3844	T15527	T15520	punct	", ",are
R3845	T15528	T15529	advmod	almost,certainly
R3846	T15529	T15520	advmod	certainly,are
R3847	T15530	T15531	amod	functional,nulls
R3848	T15531	T15520	attr	nulls,are
R3849	T15532	T15520	punct	.,are
R385	T1363	T1362	punct	", ",transducers
R3850	T15534	T15535	prep	In,shown
R3851	T15536	T15534	pobj	Drosophila,In
R3852	T15537	T15535	punct	", ",shown
R3853	T15538	T15535	nsubjpass	it,shown
R3854	T15539	T15535	aux	has,shown
R3855	T15540	T15535	auxpass	been,shown
R3856	T15541	T15542	mark	that,is
R3857	T15542	T15535	ccomp	is,shown
R3858	T15543	T15544	det	the,domain
R3859	T15544	T15542	nsubj	domain,is
R386	T1364	T1362	cc	or,transducers
R3860	T15545	T15544	compound	PHD,domain
R3861	T15546	T15547	advmod	absolutely,necessary
R3862	T15547	T15542	acomp	necessary,is
R3863	T15548	T15547	prep	for,necessary
R3864	T15549	T15550	compound	Pygopus,function
R3865	T15550	T15548	pobj	function,for
R3866	T15551	T15550	prep	in,function
R3867	T15552	T15553	compound	Wg,signaling
R3868	T15553	T15551	pobj	signaling,in
R3869	T15554	T15535	punct	.,shown
R387	T1365	T1362	conj	modulators,transducers
R3870	T15556	T15557	det	The,domain
R3871	T15557	T15559	nsubj	domain,is
R3872	T15558	T15557	compound	PHD,domain
R3873	T15560	T15561	nummod	60,acids
R3874	T15561	T15559	attr	acids,is
R3875	T15562	T15561	compound	amino,acids
R3876	T15563	T15561	prep	with,acids
R3877	T15564	T15565	nummod	seven,cysteines
R3878	T15565	T15563	pobj	cysteines,with
R3879	T15566	T15565	cc	and,cysteines
R388	T1366	T1362	punct	", ",transducers
R3880	T15567	T15568	det	a,histidine
R3881	T15568	T15565	conj	histidine,cysteines
R3882	T15569	T15568	punct	", ",histidine
R3883	T15570	T15568	acl	predicted,histidine
R3884	T15571	T15572	aux	to,chelate
R3885	T15572	T15570	xcomp	chelate,predicted
R3886	T15573	T15574	nummod	two,ions
R3887	T15574	T15572	dobj	ions,chelate
R3888	T15575	T15574	compound	zinc,ions
R3889	T15576	T15559	punct	.,is
R389	T1367	T1362	prep	of,transducers
R3890	T15578	T15579	compound	PHD,domains
R3891	T15579	T15580	nsubjpass	domains,found
R3892	T15581	T15580	auxpass	are,found
R3893	T15582	T15580	prep	in,found
R3894	T15583	T15584	amod	diverse,proteins
R3895	T15584	T15582	pobj	proteins,in
R3896	T15585	T15584	punct	", ",proteins
R3897	T15586	T15584	prep	including,proteins
R3898	T15587	T15588	compound	transcription,factors
R3899	T15588	T15586	pobj	factors,including
R39	T984	T986	advcl	understand,made
R390	T1368	T1369	compound	Wnt,signal
R3900	T15589	T15580	punct	", ",found
R3901	T15590	T15580	cc	and,found
R3902	T15591	T15592	aux	have,implicated
R3903	T15592	T15580	conj	implicated,found
R3904	T15593	T15592	auxpass	been,implicated
R3905	T15594	T15592	prep	in,implicated
R3906	T15595	T15596	compound	chromatin,remodeling
R3907	T15596	T15594	pobj	remodeling,in
R3908	T15597	T15596	cc	and,remodeling
R3909	T15598	T15599	compound	protein,protein
R391	T1369	T1370	compound	signal,intensity
R3910	T15599	T15601	compound	protein,interactions
R3911	T15600	T15599	punct	-,protein
R3912	T15601	T15596	conj	interactions,remodeling
R3913	T15602	T15603	punct	[,8
R3914	T15603	T15592	parataxis	8,implicated
R3915	T15604	T15603	punct	],8
R3916	T15605	T15580	punct	.,found
R3917	T15607	T15608	prep	In,loses
R3918	T15609	T15607	pobj	Drosophila,In
R3919	T15610	T15611	det	the,allele
R392	T1370	T1367	pobj	intensity,of
R3920	T15611	T15608	nsubj	allele,loses
R3921	T15612	T15611	compound	pygoF107,allele
R3922	T15613	T15611	punct	", ",allele
R3923	T15614	T15611	prep	with,allele
R3924	T15615	T15616	det	a,mutation
R3925	T15616	T15619	nsubj	mutation,converting
R3926	T15617	T15616	amod	single,mutation
R3927	T15618	T15616	compound	missense,mutation
R3928	T15619	T15614	pcomp	converting,with
R3929	T15620	T15621	compound	amino,acid
R393	T1371	T1337	punct	.,required
R3930	T15621	T15619	dobj	acid,converting
R3931	T15622	T15621	nummod	802,acid
R3932	T15623	T15621	prep	in,acid
R3933	T15624	T15625	det	the,domain
R3934	T15625	T15623	pobj	domain,in
R3935	T15626	T15625	compound	PHD,domain
R3936	T15627	T15619	prep	from,converting
R3937	T15628	T15627	pobj	cysteine,from
R3938	T15629	T15627	prep	to,from
R3939	T15630	T15629	pobj	tyrosine,to
R3940	T15631	T15608	punct	", ",loses
R3941	T15632	T15633	compound	Wnt,signaling
R3942	T15633	T15634	compound	signaling,function
R3943	T15634	T15608	dobj	function,loses
R3944	T15635	T15608	prep	in,loses
R3945	T15636	T15637	preconj	both,embryogenesis
R3946	T15637	T15635	pobj	embryogenesis,in
R3947	T15638	T15637	cc	and,embryogenesis
R3948	T15639	T15640	amod	imaginal,disc
R3949	T15640	T15641	compound	disc,development
R3950	T15641	T15637	conj	development,embryogenesis
R3951	T15642	T15643	punct	[,8
R3952	T15643	T15608	parataxis	8,loses
R3953	T15644	T15643	punct	],8
R3954	T15645	T15608	punct	.,loses
R3955	T15647	T15648	det	The,alleles
R3956	T15648	T15653	nsubj	alleles,carried
R3957	T15649	T15650	compound	Pygo1,Pygo2
R3958	T15650	T15648	compound	Pygo2,alleles
R3959	T15651	T15650	punct	/,Pygo2
R3960	T15652	T15648	compound	mutant,alleles
R3961	T15654	T15648	acl	made,alleles
R3962	T15655	T15654	prep	in,made
R3963	T15656	T15657	det	this,report
R3964	T15657	T15655	pobj	report,in
R3965	T15658	T15653	dobj	deletions,carried
R3966	T15659	T15658	prep	of,deletions
R3967	T15660	T15661	det	the,domains
R3968	T15661	T15659	pobj	domains,of
R3969	T15662	T15661	amod	entire,domains
R3970	T15663	T15661	compound	PHD,domains
R3971	T15664	T15661	punct	", ",domains
R3972	T15665	T15666	advmod	as,as
R3973	T15666	T15661	cc	as,domains
R3974	T15667	T15666	advmod	well,as
R3975	T15668	T15669	amod	most,sequences
R3976	T15669	T15661	conj	sequences,domains
R3977	T15670	T15669	amod	other,sequences
R3978	T15671	T15669	compound	coding,sequences
R3979	T15672	T15653	punct	.,carried
R398	T2638	T2639	compound	Wnt,signaling
R3980	T15674	T15675	prep	For,deleted
R3981	T15676	T15677	det	the,gene
R3982	T15677	T15674	pobj	gene,For
R3983	T15678	T15677	compound	Pygo1,gene
R3984	T15679	T15675	punct	", ",deleted
R3985	T15680	T15681	det	the,region
R3986	T15681	T15675	nsubjpass	region,deleted
R3987	T15682	T15681	compound	coding,region
R3988	T15683	T15681	prep	for,region
R3989	T15684	T15685	quantmod	372,417
R399	T2639	T2640	nsubj	signaling,is
R3990	T15685	T15687	nummod	417,acids
R3991	T15686	T15685	quantmod	of,417
R3992	T15687	T15683	pobj	acids,for
R3993	T15688	T15687	amod	total,acids
R3994	T15689	T15687	compound	amino,acids
R3995	T15690	T15675	auxpass	was,deleted
R3996	T15691	T15675	punct	", ",deleted
R3997	T15692	T15675	cc	and,deleted
R3998	T15693	T15694	prep	for,deleted
R3999	T15694	T15675	conj	deleted,deleted
R40	T985	T984	advmod	better,understand
R400	T2641	T2640	prep	of,is
R4000	T15695	T15696	det	the,gene
R4001	T15696	T15693	pobj	gene,for
R4002	T15697	T15696	compound	Pygo2,gene
R4003	T15698	T15694	punct	", ",deleted
R4004	T15699	T15694	nsubj	we,deleted
R4005	T15700	T15694	dobj	coding,deleted
R4006	T15701	T15700	prep	for,coding
R4007	T15702	T15703	quantmod	354,405
R4008	T15703	T15705	nummod	405,acids
R4009	T15704	T15703	quantmod	of,405
R401	T2642	T2643	amod	critical,importance
R4010	T15705	T15701	pobj	acids,for
R4011	T15706	T15705	compound	amino,acids
R4012	T15707	T15694	punct	.,deleted
R4013	T15709	T15710	nsubj	It,is
R4014	T15711	T15710	advmod	therefore,is
R4015	T15712	T15713	advmod	very,unlikely
R4016	T15713	T15710	acomp	unlikely,is
R4017	T15714	T15715	mark	that,were
R4018	T15715	T15710	ccomp	were,is
R4019	T15716	T15717	det	the,phenotypes
R402	T2643	T2641	pobj	importance,of
R4020	T15717	T15715	nsubj	phenotypes,were
R4021	T15718	T15719	advmod	relatively,mild
R4022	T15719	T15717	amod	mild,phenotypes
R4023	T15720	T15717	acl	observed,phenotypes
R4024	T15721	T15722	det	the,result
R4025	T15722	T15715	attr	result,were
R4026	T15723	T15722	prep	of,result
R4027	T15724	T15725	amod	residual,function
R4028	T15725	T15723	pobj	function,of
R4029	T15726	T15725	prep	of,function
R403	T2644	T2640	prep	in,is
R4030	T15727	T15728	det	the,alleles
R4031	T15728	T15726	pobj	alleles,of
R4032	T15729	T15728	amod	targeted,alleles
R4033	T15730	T15710	punct	.,is
R4034	T15732	T15733	nsubj	We,focused
R4035	T15734	T15735	poss	our,analysis
R4036	T15735	T15733	dobj	analysis,focused
R4037	T15736	T15733	prep	on,focused
R4038	T15737	T15738	det	the,kidney
R4039	T15738	T15736	pobj	kidney,on
R404	T2645	T2646	amod	several,stages
R4040	T15739	T15738	amod	developing,kidney
R4041	T15740	T15738	punct	", ",kidney
R4042	T15741	T15742	prep	in,shown
R4043	T15742	T15738	relcl	shown,kidney
R4044	T15743	T15741	pobj	which,in
R4045	T15744	T15745	compound	Wnt,signaling
R4046	T15745	T15742	nsubjpass	signaling,shown
R4047	T15746	T15742	aux	has,shown
R4048	T15747	T15742	auxpass	been,shown
R4049	T15748	T15749	aux	to,be
R405	T2646	T2644	pobj	stages,in
R4050	T15749	T15742	xcomp	be,shown
R4051	T15750	T15749	prep	of,be
R4052	T15751	T15752	amod	critical,importance
R4053	T15752	T15750	pobj	importance,of
R4054	T15753	T15749	prep	in,be
R4055	T15754	T15755	amod	several,stages
R4056	T15755	T15753	pobj	stages,in
R4057	T15756	T15755	prep	of,stages
R4058	T15757	T15756	pobj	nephrogenesis,of
R4059	T15758	T15733	punct	.,focused
R406	T2647	T2646	prep	of,stages
R4060	T15760	T15761	nsubjpass	Wnt9b,made
R4061	T15762	T15761	auxpass	is,made
R4062	T15763	T15761	agent	by,made
R4063	T15764	T15765	det	the,bud
R4064	T15765	T15763	pobj	bud,by
R4065	T15766	T15765	amod	ureteric,bud
R4066	T15767	T15761	cc	and,made
R4067	T15768	T15761	conj	induces,made
R4068	T15769	T15770	det	the,mesenchyme
R4069	T15770	T15768	dobj	mesenchyme,induces
R407	T2648	T2649	compound	kidney,development
R4070	T15771	T15770	amod	metanephric,mesenchyme
R4071	T15772	T15773	aux	to,undergo
R4072	T15773	T15768	xcomp	undergo,induces
R4073	T15774	T15773	dobj	nephrogenesis,undergo
R4074	T15775	T15776	punct	[,2
R4075	T15776	T15773	parataxis	2,undergo
R4076	T15777	T15776	punct	],2
R4077	T15778	T15761	punct	.,made
R4078	T15780	T15781	advmod	Downstream,is
R4079	T15782	T15780	prep	of,Downstream
R408	T2649	T2647	pobj	development,of
R4080	T15783	T15782	pobj	Wnt9b,of
R4081	T15784	T15781	nsubj	Wnt4,is
R4082	T15785	T15786	punct	[,2
R4083	T15786	T15784	parataxis	2,Wnt4
R4084	T15787	T15786	punct	],2
R4085	T15788	T15784	punct	", ",Wnt4
R4086	T15789	T15790	dep	which,made
R4087	T15790	T15784	relcl	made,Wnt4
R4088	T15791	T15790	auxpass	is,made
R4089	T15792	T15790	agent	by,made
R409	T2650	T2640	punct	.,is
R4090	T15793	T15794	det	the,mesenchyme
R4091	T15794	T15792	pobj	mesenchyme,by
R4092	T15795	T15794	amod	metanephric,mesenchyme
R4093	T15796	T15790	punct	", ",made
R4094	T15797	T15790	cc	and,made
R4095	T15798	T15799	auxpass	is,required
R4096	T15799	T15790	conj	required,made
R4097	T15800	T15799	advmod	also,required
R4098	T15801	T15799	prep	for,required
R4099	T15802	T15801	pobj	nephrogenesis,for
R41	T987	T988	det	the,functions
R410	T2652	T2653	amod	Mutual,interactions
R4100	T15803	T15804	punct	[,3
R4101	T15804	T15799	parataxis	3,required
R4102	T15805	T15804	punct	],3
R4103	T15806	T15781	punct	.,is
R4104	T15808	T15809	prep	In,produced
R4105	T15810	T15808	pobj	addition,In
R4106	T15811	T15809	punct	", ",produced
R4107	T15812	T15809	nsubjpass	Wnt11,produced
R4108	T15813	T15809	auxpass	is,produced
R4109	T15814	T15809	agent	by,produced
R411	T2653	T2655	nsubj	interactions,drive
R4110	T15815	T15816	det	the,tips
R4111	T15816	T15814	pobj	tips,by
R4112	T15817	T15818	amod	ureteric,bud
R4113	T15818	T15816	compound	bud,tips
R4114	T15819	T15809	cc	and,produced
R4115	T15820	T15809	conj	induces,produced
R4116	T15821	T15822	compound	GDNF,expression
R4117	T15822	T15820	dobj	expression,induces
R4118	T15823	T15820	prep	in,induces
R4119	T15824	T15825	det	the,mesenchyme
R412	T2654	T2653	amod	inductive,interactions
R4120	T15825	T15823	pobj	mesenchyme,in
R4121	T15826	T15825	amod	metanephric,mesenchyme
R4122	T15827	T15828	punct	[,4
R4123	T15828	T15820	parataxis	4,induces
R4124	T15829	T15828	punct	],4
R4125	T15830	T15809	punct	.,produced
R4126	T15832	T15833	prep	In,describe
R4127	T15834	T15835	det	this,report
R4128	T15835	T15832	pobj	report,In
R4129	T15836	T15833	nsubj	we,describe
R413	T2656	T2653	prep	between,interactions
R4130	T15837	T15838	det	a,function
R4131	T15838	T15833	dobj	function,describe
R4132	T15839	T15838	amod	novel,function
R4133	T15840	T15838	compound	Wnt,function
R4134	T15841	T15838	prep	in,function
R4135	T15842	T15843	compound	kidney,development
R4136	T15843	T15841	pobj	development,in
R4137	T15844	T15833	punct	.,describe
R4138	T15846	T15847	det	The,reporter
R4139	T15847	T15852	nsubj	reporter,indicated
R414	T2657	T2658	det	the,bud
R4140	T15848	T15849	compound	BAT,gal
R4141	T15849	T15851	compound	gal,transgene
R4142	T15850	T15849	punct	-,gal
R4143	T15851	T15847	compound	transgene,reporter
R4144	T15853	T15854	det	the,presence
R4145	T15854	T15852	dobj	presence,indicated
R4146	T15855	T15854	prep	of,presence
R4147	T15856	T15857	amod	canonical,signaling
R4148	T15857	T15855	pobj	signaling,of
R4149	T15858	T15857	compound	Wnt,signaling
R415	T2658	T2656	pobj	bud,between
R4150	T15859	T15854	prep	in,presence
R4151	T15860	T15861	det	the,bud
R4152	T15861	T15859	pobj	bud,in
R4153	T15862	T15861	amod	ureteric,bud
R4154	T15863	T15854	cc	and,presence
R4155	T15864	T15865	poss	its,derivatives
R4156	T15865	T15854	conj	derivatives,presence
R4157	T15866	T15865	prep	in,derivatives
R4158	T15867	T15868	det	the,kidney
R4159	T15868	T15866	pobj	kidney,in
R416	T2659	T2658	amod	ureteric,bud
R4160	T15869	T15868	amod	developing,kidney
R4161	T15870	T15852	punct	.,indicated
R4162	T15872	T15873	advmod	Further,lost
R4163	T15874	T15873	punct	", ",lost
R4164	T15875	T15873	prep	in,lost
R4165	T15876	T15877	det	the,mutants
R4166	T15877	T15875	pobj	mutants,in
R4167	T15878	T15877	compound	Pygo2,mutants
R4168	T15879	T15880	det	this,signal
R4169	T15880	T15873	nsubjpass	signal,lost
R417	T2660	T2658	cc	and,bud
R4170	T15881	T15873	auxpass	was,lost
R4171	T15882	T15873	punct	", ",lost
R4172	T15883	T15873	advcl	suggesting,lost
R4173	T15884	T15885	amod	significant,reduction
R4174	T15885	T15883	dobj	reduction,suggesting
R4175	T15993	T15983	acomp	similar,is
R4176	T15994	T15993	prep	to,similar
R4177	T15995	T15994	pobj	that,to
R4178	T15996	T15997	advmod	previously,reported
R4179	T15997	T15995	acl	reported,that
R418	T2661	T2662	amod	metanephric,mesenchyme
R4180	T15998	T15997	prep	for,reported
R4181	T15999	T16000	compound	Wnt11,mutants
R4182	T16000	T15998	pobj	mutants,for
R4183	T16001	T16002	punct	[,4
R4184	T16002	T15983	parataxis	4,is
R4185	T16003	T16002	punct	],4
R4186	T16004	T15983	punct	.,is
R4187	T16006	T16007	det	The,mechanisms
R4188	T16007	T16009	nsubj	mechanisms,are
R4189	T16008	T16007	amod	underlying,mechanisms
R419	T2662	T2658	conj	mesenchyme,bud
R4190	T15886	T15885	prep	of,reduction
R4191	T16010	T16009	punct	", ",are
R4192	T15887	T15888	compound	Wnt,signaling
R4193	T15888	T15886	pobj	signaling,of
R4194	T16011	T16009	advmod	however,are
R4195	T15889	T15873	punct	.,lost
R4196	T16012	T16009	punct	", ",are
R4197	T15891	T15892	prep	In,observed
R4198	T15893	T15891	pobj	addition,In
R4199	T16013	T16009	acomp	likely,are
R42	T988	T984	dobj	functions,understand
R420	T2663	T2655	dobj	nephrogenesis,drive
R4200	T15894	T15892	punct	", ",observed
R4201	T15895	T15892	nsubj	we,observed
R4202	T15896	T15897	det	a,decrease
R4203	T16014	T16015	aux	to,be
R4204	T15897	T15892	dobj	decrease,observed
R4205	T15898	T15897	amod	resulting,decrease
R4206	T16015	T16013	xcomp	be,likely
R4207	T15899	T15897	prep	in,decrease
R4208	T15900	T15901	amod	ureteric,tip
R4209	T15901	T15902	compound	tip,density
R421	T2664	T2665	punct	[,1
R4210	T16016	T16015	acomp	distinct,be
R4211	T15902	T15899	pobj	density,in
R4212	T15903	T15897	punct	", ",decrease
R4213	T15904	T15905	amod	reduced,size
R4214	T16017	T16009	punct	.,are
R4215	T15905	T15897	conj	size,decrease
R4216	T15906	T15905	compound	kidney,size
R4217	T15907	T15905	cc	and,size
R4218	T16019	T16020	nsubjpass	Wnt11,thought
R4219	T15908	T15909	amod	altered,morphology
R422	T2665	T2655	parataxis	1,drive
R4220	T15909	T15905	conj	morphology,size
R4221	T15910	T15909	prep	of,morphology
R4222	T15911	T15912	det	the,tree
R4223	T16021	T16020	auxpass	is,thought
R4224	T15912	T15910	pobj	tree,of
R4225	T15913	T15912	amod	ureteric,tree
R4226	T15914	T15892	prep	in,observed
R4227	T15915	T15914	pobj	mutants,in
R4228	T16022	T16020	advmod	generally,thought
R4229	T15916	T15892	punct	", ",observed
R423	T2666	T2665	punct	],1
R4230	T15917	T15892	advcl	indicating,observed
R4231	T16023	T16024	aux	to,act
R4232	T15918	T15919	det	a,role
R4233	T15919	T15917	dobj	role,indicating
R4234	T15920	T15919	prep	for,role
R4235	T16024	T16020	xcomp	act,thought
R4236	T15921	T15922	amod	canonical,signaling
R4237	T15922	T15920	pobj	signaling,for
R4238	T15923	T15922	compound	Wnt,signaling
R4239	T15924	T15919	prep	in,role
R424	T2667	T2655	punct	.,drive
R4240	T16025	T16024	prep	through,act
R4241	T15925	T15926	amod	branching,morphogenesis
R4242	T15926	T15924	pobj	morphogenesis,in
R4243	T15927	T15926	prep	of,morphogenesis
R4244	T15928	T15929	det	the,bud
R4245	T15929	T15927	pobj	bud,of
R4246	T15930	T15929	amod	ureteric,bud
R4247	T16026	T16027	det	a,pathway
R4248	T15931	T15892	punct	.,observed
R4249	T15933	T15934	mark	While,is
R425	T2669	T2670	det	The,bud
R4250	T16027	T16025	pobj	pathway,through
R4251	T15934	T15938	advcl	is,remains
R4252	T15935	T15936	det	the,interpretation
R4253	T15936	T15934	nsubj	interpretation,is
R4254	T16028	T16027	amod	noncanonical,pathway
R4255	T15937	T15936	amod	simplest,interpretation
R4256	T16029	T16024	punct	", ",act
R4257	T15939	T15940	amod	direct,signaling
R4258	T15940	T15934	attr	signaling,is
R4259	T15941	T15940	compound	Wnt,signaling
R426	T2670	T2672	nsubj	bud,synthesizes
R4260	T16030	T16024	punct	(,act
R4261	T15942	T15940	prep	to,signaling
R4262	T15943	T15944	det	the,bud
R4263	T15944	T15942	pobj	bud,to
R4264	T16031	T16032	mark	although,noted
R4265	T15945	T15944	amod	ureteric,bud
R4266	T15946	T15938	punct	", ",remains
R4267	T16032	T16024	advcl	noted,act
R4268	T15947	T15938	nsubj	it,remains
R4269	T15948	T15938	oprd	possible,remains
R427	T2671	T2670	amod	ureteric,bud
R4270	T15949	T15950	mark	that,are
R4271	T16033	T16032	nsubjpass	exceptions,noted
R4272	T15950	T15938	ccomp	are,remains
R4273	T15951	T15952	det	the,abnormalities
R4274	T15952	T15950	nsubj	abnormalities,are
R4275	T16034	T16032	aux	have,noted
R4276	T15953	T15952	amod	observed,abnormalities
R4277	T15954	T15955	det	the,result
R4278	T16035	T16032	auxpass	been,noted
R4279	T15955	T15950	attr	result,are
R428	T2673	T2672	dobj	Wnt9b,synthesizes
R4280	T15956	T15955	prep	of,result
R4281	T15957	T15958	amod	indirect,effects
R4282	T16036	T16037	punct	[,31
R4283	T16037	T16032	parataxis	31,noted
R4284	T15958	T15956	pobj	effects,of
R4285	T16038	T16037	punct	],31
R4286	T15959	T15950	punct	", ",are
R4287	T15960	T15950	prep	with,are
R4288	T16039	T16024	punct	),act
R4289	T15961	T15962	amod	altered,signaling
R429	T2674	T2673	punct	", ",Wnt9b
R4290	T15962	T15964	nsubj	signaling,affecting
R4291	T16040	T16024	punct	", ",act
R4292	T15963	T15962	compound	Wnt,signaling
R4293	T15964	T15960	pcomp	affecting,with
R4294	T16041	T16042	mark	whereas,promote
R4295	T15965	T15962	prep	to,signaling
R4296	T15966	T15967	det	the,mesenchyme
R4297	T15967	T15965	pobj	mesenchyme,to
R4298	T15968	T15967	amod	metanephric,mesenchyme
R4299	T15969	T15964	advmod	then,affecting
R43	T989	T988	prep	of,functions
R430	T2675	T2676	dep	which,is
R4300	T15970	T15964	dobj	mesenchyme,affecting
R4301	T16042	T16024	advcl	promote,act
R4302	T15971	T15970	prep	to,mesenchyme
R4303	T15972	T15973	amod	ureteric,bud
R4304	T15973	T15974	compound	bud,signaling
R4305	T16043	T16044	det	the,genes
R4306	T15974	T15971	pobj	signaling,to
R4307	T15975	T15938	punct	.,remains
R4308	T16044	T16042	nsubj	genes,promote
R4309	T15977	T15978	det	The,phenotype
R431	T2676	T2673	relcl	is,Wnt9b
R4310	T15978	T15983	nsubj	phenotype,is
R4311	T15979	T15980	compound	Pygo1,Pygo2
R4312	T16045	T16044	compound	Pygopus,genes
R4313	T15980	T15982	compound	Pygo2,mutant
R4314	T15981	T15980	punct	/,Pygo2
R4315	T16046	T16047	amod	canonical,signaling
R4316	T15982	T15978	compound	mutant,phenotype
R4317	T15984	T15978	prep	of,phenotype
R4318	T16047	T16042	dobj	signaling,promote
R4319	T15985	T15986	amod	reduced,morphogenesis
R432	T2677	T2676	acomp	essential,is
R4320	T15986	T15984	pobj	morphogenesis,of
R4321	T15987	T15986	compound	branching,morphogenesis
R4322	T16048	T16047	compound	Wnt,signaling
R4323	T15988	T15986	prep	of,morphogenesis
R4324	T15989	T15990	det	the,bud
R4325	T15990	T15988	pobj	bud,of
R4326	T16049	T16020	punct	.,thought
R4327	T15991	T15990	amod	ureteric,bud
R4328	T15992	T15993	advmod	surprisingly,similar
R4329	T16051	T16052	advmod	Further,showed
R433	T2678	T2677	prep	for,essential
R4330	T16053	T16052	punct	", ",showed
R4331	T16054	T16055	det	the,mutants
R4332	T16055	T16052	nsubj	mutants,showed
R4333	T16056	T16055	compound	Wnt11,mutants
R4334	T16100	T16101	det	The,mutants
R4335	T16057	T16058	det	an,loop
R4336	T16101	T16105	nsubj	mutants,showed
R4337	T16102	T16101	nmod	Pygo1,mutants
R4338	T16058	T16052	dobj	loop,showed
R4339	T16103	T16102	punct	/,Pygo1
R434	T2679	T2678	pobj	induction,for
R4340	T16104	T16102	nummod	2,Pygo1
R4341	T16059	T16058	amod	altered,loop
R4342	T16106	T16105	advmod	also,showed
R4343	T16107	T16108	det	an,expansion
R4344	T16108	T16105	dobj	expansion,showed
R4345	T16060	T16058	compound	feedback,loop
R4346	T16109	T16108	prep	of,expansion
R4347	T16110	T16111	det	the,zone
R4348	T16111	T16109	pobj	zone,of
R4349	T16112	T16111	prep	of,zone
R435	T2680	T2679	prep	of,induction
R4350	T16113	T16114	amod	thickened,mesenchyme
R4351	T16061	T16058	prep	between,loop
R4352	T16062	T16063	compound	Wnt,signaling
R4353	T16114	T16112	pobj	mesenchyme,of
R4354	T16063	T16061	pobj	signaling,between
R4355	T16115	T16116	dep	that,caps
R4356	T16064	T16063	prep	from,signaling
R4357	T16116	T16111	relcl	caps,zone
R4358	T16117	T16118	det	the,bud
R4359	T16118	T16116	dobj	bud,caps
R436	T2681	T2682	det	the,mesenchyme
R4360	T16065	T16066	det	the,bud
R4361	T16119	T16118	amod	ureteric,bud
R4362	T16120	T16105	punct	.,showed
R4363	T16066	T16064	pobj	bud,from
R4364	T16122	T16123	advmod	Nevertheless,formed
R4365	T16067	T16066	amod	ureteric,bud
R4366	T16124	T16123	punct	", ",formed
R4367	T16125	T16126	det	the,mesenchyme
R4368	T16126	T16123	nsubj	mesenchyme,formed
R4369	T16068	T16064	prep	to,from
R437	T2682	T2680	pobj	mesenchyme,of
R4370	T16127	T16126	nmod	mutant,mesenchyme
R4371	T16128	T16126	amod	metanephric,mesenchyme
R4372	T16069	T16070	det	the,mesenchyme
R4373	T16129	T16123	dobj	nephrons,formed
R4374	T16130	T16123	advmod	normally,formed
R4375	T16131	T16123	punct	.,formed
R4376	T16070	T16068	pobj	mesenchyme,to
R4377	T16071	T16063	cc	and,signaling
R4378	T16133	T16134	nsubj	This,was
R4379	T16072	T16073	compound	GDNF,signaling
R438	T2683	T2684	aux	to,form
R4380	T16135	T16136	advmod	particularly,interesting
R4381	T16073	T16063	conj	signaling,signaling
R4382	T16136	T16134	acomp	interesting,was
R4383	T16137	T16134	punct	", ",was
R4384	T16138	T16139	mark	as,shown
R4385	T16074	T16073	prep	from,signaling
R4386	T16139	T16134	advcl	shown,was
R4387	T16075	T16076	det	the,mesenchyme
R4388	T16140	T16141	compound	Wnt9b,signaling
R4389	T16141	T16139	nsubjpass	signaling,shown
R439	T2684	T2679	acl	form,induction
R4390	T16142	T16141	prep	from,signaling
R4391	T16076	T16074	pobj	mesenchyme,from
R4392	T16143	T16144	det	the,bud
R4393	T16144	T16142	pobj	bud,from
R4394	T16145	T16144	amod	ureteric,bud
R4395	T16077	T16074	prep	to,from
R4396	T16146	T16142	prep	to,from
R4397	T16147	T16148	det	the,mesenchyme
R4398	T16078	T16079	det	the,bud
R4399	T16148	T16146	pobj	mesenchyme,to
R44	T990	T991	npadvmod	Pygopus,mediated
R440	T2685	T2684	dobj	nephrons,form
R4400	T16149	T16148	amod	metanephric,mesenchyme
R4401	T16150	T16139	aux	has,shown
R4402	T16151	T16139	auxpass	been,shown
R4403	T16152	T16153	aux	to,induce
R4404	T16153	T16139	xcomp	induce,shown
R4405	T16079	T16077	pobj	bud,to
R4406	T16154	T16153	dobj	nephrogenesis,induce
R4407	T16155	T16153	prep	via,induce
R4408	T16156	T16157	amod	canonical,signaling
R4409	T16080	T16052	punct	", ",showed
R441	T2686	T2687	punct	[,2
R4410	T16157	T16155	pobj	signaling,via
R4411	T16158	T16157	compound	Wnt,signaling
R4412	T16159	T16160	punct	[,2
R4413	T16081	T16082	mark	whereas,observed
R4414	T16160	T16153	parataxis	2,induce
R4415	T16161	T16160	punct	],2
R4416	T16162	T16134	punct	.,was
R4417	T16082	T16052	advcl	observed,showed
R4418	T16164	T16165	det	The,results
R4419	T16083	T16082	prep	in,observed
R442	T2687	T2672	parataxis	2,synthesizes
R4420	T16165	T16166	nsubj	results,indicate
R4421	T16167	T16165	prep	in,results
R4422	T16084	T16085	det	the,mutants
R4423	T16168	T16169	det	this,report
R4424	T16169	T16167	pobj	report,in
R4425	T16170	T16171	det	a,divergence
R4426	T16085	T16083	pobj	mutants,in
R4427	T16171	T16166	dobj	divergence,indicate
R4428	T16172	T16171	amod	striking,divergence
R4429	T16173	T16172	cc	and,striking
R443	T2688	T2687	punct	],2
R4430	T16086	T16087	compound	Pygo1,Pygo2
R4431	T16174	T16172	conj	surprising,striking
R4432	T16175	T16171	amod	evolutionary,divergence
R4433	T16176	T16171	prep	of,divergence
R4434	T16087	T16085	compound	Pygo2,mutants
R4435	T16177	T16178	compound	Pygopus,function
R4436	T16178	T16176	pobj	function,of
R4437	T16088	T16087	punct	/,Pygo2
R4438	T16179	T16171	prep	between,divergence
R4439	T16180	T16179	pobj	Drosophila,between
R444	T2689	T2672	punct	.,synthesizes
R4440	T16181	T16180	cc	and,Drosophila
R4441	T16089	T16082	punct	", ",observed
R4442	T16182	T16180	conj	mammals,Drosophila
R4443	T16183	T16166	punct	.,indicate
R4444	T16090	T16082	nsubj	we,observed
R4445	T16185	T16186	prep	In,required
R4446	T16091	T16092	amod	disrupted,signaling
R4447	T16187	T16185	pobj	Drosophila,In
R4448	T16188	T16186	punct	", ",required
R4449	T16092	T16082	dobj	signaling,observed
R445	T2691	T2692	nsubjpass	Wnt4,made
R4450	T16189	T16190	det	the,gene
R4451	T16190	T16186	nsubjpass	gene,required
R4452	T16191	T16190	compound	Pygopus,gene
R4453	T16192	T16186	auxpass	is,required
R4454	T16193	T16186	advmod	often,required
R4455	T16194	T16186	prep	for,required
R4456	T16093	T16092	compound	Wnt,signaling
R4457	T16195	T16196	amod	canonical,signaling
R4458	T16196	T16194	pobj	signaling,for
R4459	T16197	T16196	compound	Wnt,signaling
R446	T2693	T2692	auxpass	is,made
R4460	T16094	T16082	prep	in,observed
R4461	T16198	T16186	punct	", ",required
R4462	T16199	T16200	mark	while,appear
R4463	T16200	T16186	advcl	appear,required
R4464	T16095	T16096	det	the,bud
R4465	T16201	T16200	prep	in,appear
R4466	T16202	T16201	pobj	mammals,in
R4467	T16096	T16094	pobj	bud,in
R4468	T16203	T16204	det	the,genes
R4469	T16204	T16200	nsubj	genes,appear
R447	T2694	T2692	agent	by,made
R4470	T16097	T16096	amod	ureteric,bud
R4471	T16098	T16052	punct	.,showed
R4472	T16311	T16312	amod	essential,components
R4473	T16205	T16206	compound	Pygo1,Pygo2
R4474	T16206	T16204	compound	Pygo2,genes
R4475	T16312	T16302	conj	components,modulators
R4476	T16207	T16206	punct	/,Pygo2
R4477	T16208	T16209	aux	to,play
R4478	T16313	T16290	punct	.,suggest
R4479	T16209	T16200	xcomp	play,appear
R448	T2695	T2696	det	the,mesenchyme
R4480	T16210	T16211	det	a,role
R4481	T16211	T16209	dobj	role,play
R4482	T16315	T16316	det	The,results
R4483	T16212	T16211	amod	smaller,role
R4484	T16213	T16209	prep	in,play
R4485	T16214	T16215	amod	canonical,signaling
R4486	T16215	T16213	pobj	signaling,in
R4487	T16316	T16318	nsubj	results,support
R4488	T16216	T16215	compound	Wnt,signaling
R4489	T16217	T16186	punct	.,required
R449	T2696	T2694	pobj	mesenchyme,by
R4490	T16317	T16316	compound	microarray,results
R4491	T16219	T16220	det	The,reporter
R4492	T16319	T16318	advmod	further,support
R4493	T16220	T16225	nsubj	reporter,showed
R4494	T16320	T16321	det	this,conclusion
R4495	T16221	T16222	compound	BAT,gal
R4496	T16222	T16224	compound	gal,transgene
R4497	T16321	T16318	dobj	conclusion,support
R4498	T16223	T16222	punct	-,gal
R4499	T16224	T16220	compound	transgene,reporter
R45	T991	T993	amod	mediated,signaling
R450	T2697	T2696	amod	induced,mesenchyme
R4500	T16322	T16318	punct	.,support
R4501	T16226	T16220	prep	of,reporter
R4502	T16227	T16228	amod	canonical,signaling
R4503	T16324	T16325	mark	Whereas,monitors
R4504	T16228	T16226	pobj	signaling,of
R4505	T16229	T16228	compound	Wnt,signaling
R4506	T16230	T16231	amod	reduced,signal
R4507	T16231	T16225	dobj	signal,showed
R4508	T16232	T16230	cc	but,reduced
R4509	T16233	T16232	advmod	generally,but
R451	T2698	T2696	amod	metanephric,mesenchyme
R4510	T16234	T16232	neg	not,but
R4511	T16325	T16332	advcl	monitors,allows
R4512	T16235	T16230	conj	absent,reduced
R4513	T16236	T16231	prep	in,signal
R4514	T16326	T16327	det	the,reporter
R4515	T16237	T16238	compound	Pygo1,Pygo2
R4516	T16238	T16240	compound	Pygo2,embryos
R4517	T16239	T16238	punct	/,Pygo2
R4518	T16327	T16325	nsubj	reporter,monitors
R4519	T16240	T16236	pobj	embryos,in
R452	T2699	T2692	cc	and,made
R4520	T16241	T16240	compound	mutant,embryos
R4521	T16242	T16231	punct	", ",signal
R4522	T16328	T16329	compound	BAT,gal
R4523	T16243	T16231	prep	with,signal
R4524	T16244	T16245	npadvmod	tissue,specific
R4525	T16245	T16247	amod	specific,variation
R4526	T16329	T16331	compound	gal,transgene
R4527	T16246	T16245	punct	-,specific
R4528	T16247	T16243	pobj	variation,with
R4529	T16248	T16247	prep	in,variation
R453	T2700	T2701	auxpass	is,required
R4530	T16330	T16329	punct	-,gal
R4531	T16249	T16248	pobj	level,in
R4532	T16250	T16249	prep	of,level
R4533	T16251	T16250	pobj	diminution,of
R4534	T16331	T16327	compound	transgene,reporter
R4535	T16252	T16225	punct	.,showed
R4536	T16333	T16334	det	the,level
R4537	T16254	T16255	prep	In,were
R4538	T16256	T16254	pobj	addition,In
R4539	T16257	T16255	punct	", ",were
R454	T2701	T2692	conj	required,made
R4540	T16334	T16325	dobj	level,monitors
R4541	T16258	T16259	det	the,kidneys
R4542	T16259	T16255	nsubj	kidneys,were
R4543	T16260	T16255	neg	not,were
R4544	T16335	T16334	compound	expression,level
R4545	T16261	T16255	acomp	unique,were
R4546	T16262	T16255	prep	in,were
R4547	T16263	T16262	pcomp	showing,in
R4548	T16336	T16334	prep	of,level
R4549	T16264	T16265	advmod	surprisingly,normal
R455	T2702	T2701	advmod	also,required
R4550	T16265	T16266	amod	normal,development
R4551	T16266	T16263	dobj	development,showing
R4552	T16337	T16338	advmod	only,one
R4553	T16267	T16266	prep	in,development
R4554	T16268	T16269	compound	Pygo1,Pygo2
R4555	T16269	T16271	compound	Pygo2,mutants
R4556	T16270	T16269	punct	/,Pygo2
R4557	T16271	T16267	pobj	mutants,in
R4558	T16272	T16255	punct	.,were
R4559	T16338	T16339	nummod	one,promoter
R456	T2703	T2701	prep	for,required
R4560	T16274	T16275	advmod	Indeed,proceeded
R4561	T16339	T16336	pobj	promoter,of
R4562	T16276	T16275	punct	", ",proceeded
R4563	T16277	T16275	nsubj	organogenesis,proceeded
R4564	T16278	T16275	advmod	generally,proceeded
R4565	T16340	T16341	npadvmod	Wnt,responsive
R4566	T16279	T16275	prep	without,proceeded
R4567	T16280	T16281	amod	detectable,abnormality
R4568	T16281	T16279	pobj	abnormality,without
R4569	T16341	T16339	amod	responsive,promoter
R457	T2704	T2703	pobj	nephrogenesis,for
R4570	T16282	T16275	punct	", ",proceeded
R4571	T16283	T16275	prep	with,proceeded
R4572	T16342	T16332	punct	", ",allows
R4573	T16284	T16285	amod	few,exceptions
R4574	T16285	T16283	pobj	exceptions,with
R4575	T16286	T16275	punct	.,proceeded
R4576	T16343	T16344	det	the,microarray
R4577	T16288	T16289	det	These,results
R4578	T16289	T16290	nsubj	results,suggest
R4579	T16344	T16332	nsubj	microarray,allows
R458	T2705	T2706	punct	[,3
R4580	T16291	T16292	mark	that,are
R4581	T16345	T16346	det	the,analysis
R4582	T16292	T16290	ccomp	are,suggest
R4583	T16293	T16294	det	the,proteins
R4584	T16294	T16292	nsubj	proteins,are
R4585	T16346	T16332	dobj	analysis,allows
R4586	T16295	T16294	acl	encoded,proteins
R4587	T16296	T16295	agent	by,encoded
R4588	T16297	T16298	amod	mammalian,genes
R4589	T16298	T16296	pobj	genes,by
R459	T2706	T2701	parataxis	3,required
R4590	T16347	T16346	prep	of,analysis
R4591	T16299	T16298	compound	Pygopus,genes
R4592	T16300	T16292	advmod	often,are
R4593	T16348	T16349	det	the,levels
R4594	T16301	T16302	amod	mere,modulators
R4595	T16302	T16292	attr	modulators,are
R4596	T16303	T16302	prep	of,modulators
R4597	T16349	T16347	pobj	levels,of
R4598	T16304	T16305	amod	canonical,signaling
R4599	T16305	T16307	compound	signaling,intensity
R46	T992	T991	punct	-,mediated
R460	T2707	T2706	punct	],3
R4600	T16306	T16305	compound	Wnt,signaling
R4601	T16350	T16349	compound	activity,levels
R4602	T16307	T16303	pobj	intensity,of
R4603	T16351	T16349	prep	of,levels
R4604	T16352	T16351	pobj	promoters,of
R4605	T16308	T16302	punct	", ",modulators
R4606	T16309	T16302	cc	and,modulators
R4607	T16310	T16309	neg	not,and
R4608	T16353	T16352	prep	of,promoters
R4609	T16354	T16355	det	all,genes
R461	T2708	T2692	punct	.,made
R4610	T16355	T16353	pobj	genes,of
R4611	T16417	T16418	amod	canonical,signaling
R4612	T16356	T16332	punct	.,allows
R4613	T16418	T16416	pobj	signaling,to
R4614	T16419	T16418	compound	Wnt,signaling
R4615	T16358	T16359	advmod	Interestingly,showed
R4616	T16420	T16409	punct	", ",dedicated
R4617	T16421	T16422	mark	as,shown
R4618	T16422	T16409	advcl	shown,dedicated
R4619	T16423	T16422	aux	has,shown
R462	T2710	T2711	advmod	Furthermore,participates
R4620	T16360	T16359	punct	", ",showed
R4621	T16424	T16422	auxpass	been,shown
R4622	T16425	T16422	advmod	previously,shown
R4623	T16426	T16422	prep	in,shown
R4624	T16427	T16426	pobj	Drosophila,in
R4625	T16361	T16362	det	the,kidneys
R4626	T16428	T16407	punct	", ",represent
R4627	T16429	T16430	det	the,genes
R4628	T16362	T16359	nsubj	kidneys,showed
R4629	T16430	T16407	nsubj	genes,represent
R463	T2712	T2711	punct	", ",participates
R4630	T16431	T16430	prep	with,genes
R4631	T16432	T16433	compound	expression,differences
R4632	T16363	T16362	compound	mutant,kidneys
R4633	T16433	T16431	pobj	differences,with
R4634	T16434	T16435	compound	candidate,targets
R4635	T16364	T16365	compound	gene,expression
R4636	T16435	T16407	dobj	targets,represent
R4637	T16436	T16435	compound	Wnt,targets
R4638	T16437	T16435	punct	(,targets
R4639	T16365	T16367	compound	expression,profiles
R464	T2713	T2711	nsubj	Wnt11,participates
R4640	T16438	T16435	amod	direct,targets
R4641	T16439	T16438	cc	or,direct
R4642	T16440	T16438	conj	indirect,direct
R4643	T16366	T16365	punct	-,expression
R4644	T16441	T16435	punct	),targets
R4645	T16442	T16435	prep	in,targets
R4646	T16443	T16444	compound	kidney,development
R4647	T16367	T16359	dobj	profiles,showed
R4648	T16444	T16442	pobj	development,in
R4649	T16445	T16407	punct	.,represent
R465	T2714	T2713	punct	", ",Wnt11
R4650	T16368	T16369	advmod	surprisingly,similar
R4651	T16447	T16448	nsubj	We,predict
R4652	T16369	T16367	amod	similar,profiles
R4653	T16449	T16448	aux	would,predict
R4654	T16450	T16451	det	these,genes
R4655	T16370	T16369	prep	to,similar
R4656	T16451	T16448	dobj	genes,predict
R4657	T16452	T16453	aux	to,show
R4658	T16453	T16448	xcomp	show,predict
R4659	T16371	T16372	amod	wild,type
R466	T2715	T2713	acl	secreted,Wnt11
R4660	T16454	T16455	amod	greater,changes
R4661	T16455	T16453	dobj	changes,show
R4662	T16456	T16455	prep	in,changes
R4663	T16457	T16458	compound	expression,level
R4664	T16458	T16456	pobj	level,in
R4665	T16459	T16455	prep	in,changes
R4666	T16372	T16370	pobj	type,to
R4667	T16460	T16461	det	a,kidney
R4668	T16461	T16459	pobj	kidney,in
R4669	T16462	T16461	amod	developing,kidney
R467	T2716	T2715	agent	by,secreted
R4670	T16373	T16359	punct	.,showed
R4671	T16463	T16453	prep	with,show
R4672	T16464	T16465	det	a,removal
R4673	T16465	T16463	pobj	removal,with
R4674	T16375	T16376	det	Some,genes
R4675	T16466	T16467	advmod	more,complete
R4676	T16467	T16465	amod	complete,removal
R4677	T16468	T16465	prep	of,removal
R4678	T16376	T16377	nsubj	genes,show
R4679	T16469	T16470	amod	canonical,signaling
R468	T2717	T2718	det	the,tips
R4680	T16470	T16468	pobj	signaling,of
R4681	T16471	T16470	compound	Wnt,signaling
R4682	T16378	T16377	aux	did,show
R4683	T16472	T16448	punct	.,predict
R4684	T16379	T16377	punct	", ",show
R4685	T16380	T16377	advmod	however,show
R4686	T16381	T16377	punct	", ",show
R4687	T16382	T16383	compound	expression,differences
R4688	T16383	T16377	dobj	differences,show
R4689	T16384	T16377	punct	", ",show
R469	T2718	T2716	pobj	tips,by
R4690	T16385	T16377	cc	and,show
R4691	T16386	T16387	det	a,percentage
R4692	T16387	T16389	nsubjpass	percentage,validated
R4693	T16388	T16387	amod	high,percentage
R4694	T16389	T16377	conj	validated,show
R4695	T16390	T16387	prep	of,percentage
R4696	T16391	T16392	det	these,differences
R4697	T16392	T16390	pobj	differences,of
R4698	T16393	T16389	aux	could,validated
R4699	T16394	T16389	auxpass	be,validated
R47	T993	T989	pobj	signaling,of
R470	T2719	T2720	amod	ureteric,bud
R4700	T16395	T16389	prep	by,validated
R4701	T16396	T16397	amod	real,time
R4702	T16397	T16399	compound	time,PCR
R4703	T16398	T16397	punct	-,time
R4704	T16399	T16395	pobj	PCR,by
R4705	T16400	T16399	prep	with,PCR
R4706	T16401	T16402	amod	independent,samples
R4707	T16402	T16400	pobj	samples,with
R4708	T16403	T16402	amod	biological,samples
R4709	T16404	T16389	punct	.,validated
R471	T2720	T2718	compound	bud,tips
R4710	T16406	T16407	advcl	Assuming,represent
R4711	T16408	T16409	mark	that,dedicated
R4712	T16409	T16406	ccomp	dedicated,Assuming
R4713	T16410	T16411	det	the,genes
R4714	T16411	T16409	nsubjpass	genes,dedicated
R4715	T16412	T16411	compound	Pygopus,genes
R4716	T16413	T16411	prep	in,genes
R4717	T16414	T16413	pobj	mammals,in
R4718	T16415	T16409	auxpass	are,dedicated
R4719	T16416	T16409	prep	to,dedicated
R472	T2721	T2711	punct	", ",participates
R4723	T17129	T17130	prep	In,required
R4724	T17131	T17129	pobj	conclusion,In
R4725	T17132	T17130	punct	", ",required
R4726	T17133	T17134	det	the,gene
R4727	T17134	T17130	nsubjpass	gene,required
R4728	T17135	T17134	amod	mammalian,gene
R4729	T17136	T17134	compound	Pygo2,gene
R473	T2722	T2711	prep	in,participates
R4730	T17137	T17130	auxpass	is,required
R4731	T17138	T17130	prep	for,required
R4732	T17139	T17140	amod	normal,morphogenesis
R4733	T17140	T17138	pobj	morphogenesis,for
R4734	T17141	T17140	compound	branching,morphogenesis
R4735	T17142	T17140	prep	of,morphogenesis
R4736	T17143	T17144	det	the,bud
R4737	T17144	T17142	pobj	bud,of
R4738	T17145	T17144	amod	ureteric,bud
R4739	T17146	T17130	punct	", ",required
R474	T2723	T2724	det	a,loop
R4740	T17147	T17130	prep	with,required
R4741	T17148	T17149	nsubj	mutants,showing
R4742	T17149	T17147	pcomp	showing,with
R4743	T17150	T17151	amod	dilated,tips
R4744	T17151	T17149	dobj	tips,showing
R4745	T17152	T17151	cc	and,tips
R4746	T17153	T17154	amod	reduced,numbers
R4747	T17154	T17151	conj	numbers,tips
R4748	T17155	T17154	prep	of,numbers
R4749	T17156	T17155	pobj	tips,of
R475	T2724	T2722	pobj	loop,in
R4750	T17157	T17130	punct	.,required
R4751	T17159	T17160	prep	In,was
R4752	T17161	T17159	pobj	addition,In
R4753	T17162	T17160	punct	", ",was
R4754	T17163	T17160	prep	in,was
R4755	T17164	T17165	compound	Pygo2,mutants
R4756	T17165	T17163	pobj	mutants,in
R4757	T17166	T17160	expl	there,was
R4758	T17167	T17168	det	an,expansion
R4759	T17168	T17160	attr	expansion,was
R476	T2725	T2724	amod	positive,loop
R4760	T17169	T17168	prep	of,expansion
R4761	T17170	T17171	det	the,zone
R4762	T17171	T17169	pobj	zone,of
R4763	T17172	T17171	prep	of,zone
R4764	T17173	T17174	amod	metanephric,mesenchyme
R4765	T17174	T17172	pobj	mesenchyme,of
R4766	T17175	T17176	dep	that,caps
R4767	T17176	T17171	relcl	caps,zone
R4768	T17177	T17178	det	the,buds
R4769	T17178	T17176	dobj	buds,caps
R477	T2726	T2724	compound	feedback,loop
R4770	T17179	T17178	amod	ureteric,buds
R4771	T17180	T17160	punct	.,was
R4772	T17182	T17183	advmod	Nevertheless,proceeded
R4773	T17184	T17183	punct	", ",proceeded
R4774	T17185	T17186	compound	nephron,formation
R4775	T17186	T17183	nsubj	formation,proceeded
R4776	T17187	T17188	advmod	remarkably,normally
R4777	T17188	T17183	advmod	normally,proceeded
R4778	T17189	T17183	punct	", ",proceeded
R4779	T17190	T17191	advmod	even,in
R478	T2727	T2724	acl	promoting,loop
R4780	T17191	T17183	prep	in,proceeded
R4781	T17192	T17193	compound	Pygo1,Pygo2
R4782	T17193	T17195	npadvmod	Pygo2,homozygous
R4783	T17194	T17193	punct	/,Pygo2
R4784	T17195	T17198	amod	homozygous,mutants
R4785	T17196	T17195	amod	double,homozygous
R4786	T17197	T17195	punct	-,homozygous
R4787	T17198	T17191	pobj	mutants,in
R4788	T17199	T17183	punct	.,proceeded
R4789	T17201	T17202	nsubj	This,was
R479	T2728	T2729	amod	glial,line
R4790	T17203	T17202	acomp	surprising,was
R4791	T17204	T17202	advcl	considering,was
R4792	T17205	T17206	det	the,importance
R4793	T17206	T17204	dobj	importance,considering
R4794	T17207	T17206	prep	of,importance
R4795	T17208	T17209	det	the,gene
R4796	T17209	T17207	pobj	gene,of
R4797	T17210	T17209	compound	Pygopus,gene
R4798	T17211	T17206	prep	in,importance
R4799	T17212	T17213	amod	canonical,signaling
R48	T994	T993	amod	canonical,signaling
R480	T2729	T2731	npadvmod	line,derived
R4800	T17213	T17211	pobj	signaling,in
R4801	T17214	T17213	compound	Wnt,signaling
R4802	T17215	T17206	prep	in,importance
R4803	T17216	T17215	pobj	Drosophila,in
R4804	T17217	T17206	punct	", ",importance
R4805	T17218	T17206	cc	and,importance
R4806	T17219	T17220	det	the,importance
R4807	T17220	T17206	conj	importance,importance
R4808	T17221	T17220	prep	of,importance
R4809	T17222	T17223	amod	canonical,signaling
R481	T2730	T2729	compound	cell,line
R4810	T17223	T17221	pobj	signaling,of
R4811	T17224	T17223	compound	Wnt,signaling
R4812	T17225	T17220	prep	in,importance
R4813	T17226	T17225	pobj	nephrogenesis,in
R4814	T17227	T17202	punct	.,was
R4815	T17229	T17230	det	The,results
R4816	T17230	T17231	nsubj	results,argue
R4817	T17232	T17233	mark	that,are
R4818	T17233	T17231	ccomp	are,argue
R4819	T17234	T17235	det	the,genes
R482	T2731	T2733	amod	derived,factor
R4820	T17235	T17233	nsubj	genes,are
R4821	T17236	T17235	amod	mammalian,genes
R4822	T17237	T17235	compound	Pygopus,genes
R4823	T17238	T17233	punct	", ",are
R4824	T17239	T17233	prep	in,are
R4825	T17240	T17241	amod	most,systems
R4826	T17241	T17239	pobj	systems,in
R4827	T17242	T17241	amod	developing,systems
R4828	T17243	T17233	punct	", ",are
R4829	T17244	T17245	advmod	only,transducers
R483	T2732	T2731	punct	-,derived
R4830	T17245	T17233	attr	transducers,are
R4831	T17246	T17245	amod	quantitative,transducers
R4832	T17247	T17245	prep	of,transducers
R4833	T17248	T17249	compound	Wnt,signaling
R4834	T17249	T17247	pobj	signaling,of
R4835	T17250	T17231	punct	.,argue
R4836	T17252	T17253	amod	Previous,studies
R4837	T17253	T17256	nsubj	studies,confirm
R4838	T17254	T17255	compound	cell,culture
R4839	T17255	T17253	compound	culture,studies
R484	T2733	T2735	nmod	factor,expression
R4840	T17257	T17258	punct	[,33
R4841	T17258	T17253	parataxis	33,studies
R4842	T17259	T17258	nummod	32,33
R4843	T17260	T17258	punct	",",33
R4844	T17261	T17258	punct	],33
R4845	T17262	T17253	cc	and,studies
R4846	T17263	T17264	det	the,expression
R4847	T17264	T17253	conj	expression,studies
R4848	T17265	T17264	amod	reduced,expression
R4849	T17266	T17267	compound	BAT,gal
R485	T2734	T2733	amod	neurotrophic,factor
R4850	T17267	T17269	compound	gal,transgene
R4851	T17268	T17267	punct	-,gal
R4852	T17269	T17270	compound	transgene,reporter
R4853	T17270	T17264	compound	reporter,expression
R4854	T17271	T17253	prep	in,studies
R4855	T17272	T17273	det	the,mice
R4856	T17273	T17271	pobj	mice,in
R4857	T17274	T17275	compound	Pygo1,Pygo2
R4858	T17275	T17277	compound	Pygo2,knockout
R4859	T17276	T17275	punct	/,Pygo2
R486	T2735	T2727	dobj	expression,promoting
R4860	T17277	T17273	compound	knockout,mice
R4861	T17278	T17273	acl	described,mice
R4862	T17279	T17278	prep	in,described
R4863	T17280	T17281	det	this,report
R4864	T17281	T17279	pobj	report,in
R4865	T17282	T17256	punct	", ",confirm
R4866	T17283	T17256	aux	do,confirm
R4867	T17284	T17285	det	an,function
R4868	T17285	T17256	dobj	function,confirm
R4869	T17286	T17287	advmod	evolutionarily,conserved
R487	T2736	T2733	punct	(,factor
R4870	T17287	T17285	amod	conserved,function
R4871	T17288	T17285	prep	in,function
R4872	T17289	T17290	amod	canonical,signaling
R4873	T17290	T17288	pobj	signaling,in
R4874	T17291	T17290	compound	Wnt,signaling
R4875	T17292	T17256	punct	.,confirm
R4876	T17294	T17295	prep	In,are
R4877	T17296	T17294	pobj	mammals,In
R4878	T17297	T17295	punct	", ",are
R4879	T17298	T17295	advmod	however,are
R488	T2737	T2733	appos	GDNF,factor
R4880	T17299	T17295	punct	", ",are
R4881	T17300	T17301	det	the,effects
R4882	T17301	T17295	nsubj	effects,are
R4883	T17302	T17301	amod	phenotypic,effects
R4884	T17303	T17301	prep	of,effects
R4885	T17304	T17305	compound	Pygopus,mutation
R4886	T17305	T17303	pobj	mutation,of
R4887	T17306	T17307	advmod	much,milder
R4888	T17307	T17295	acomp	milder,are
R4889	T17308	T17307	prep	than,milder
R489	T2738	T2735	punct	),expression
R4890	T17309	T17308	prep	in,than
R4891	T17310	T17309	pobj	Drosophila,in
R4892	T17311	T17295	punct	.,are
R4893	T17313	T17314	det	The,degree
R4894	T17314	T17315	nsubj	degree,was
R4895	T17316	T17314	prep	of,degree
R4896	T17317	T17316	pobj	importance,of
R4897	T17318	T17317	prep	of,importance
R4898	T17319	T17320	det	the,genes
R4899	T17320	T17318	pobj	genes,of
R49	T995	T993	compound	Wnt,signaling
R490	T2739	T2735	prep	by,expression
R4900	T17321	T17320	nmod	Pyg1,genes
R4901	T17322	T17321	punct	/,Pyg1
R4902	T17323	T17321	nummod	2,Pyg1
R4903	T17324	T17314	prep	in,degree
R4904	T17325	T17326	compound	Wnt,signaling
R4905	T17326	T17324	pobj	signaling,in
R4906	T17327	T17328	npadvmod	context,dependent
R4907	T17328	T17315	acomp	dependent,was
R4908	T17329	T17328	punct	-,dependent
R4909	T17330	T17315	punct	", ",was
R491	T2740	T2741	det	the,mesenchyme
R4910	T17331	T17315	cc	but,was
R4911	T17332	T17333	prep	in,remained
R4912	T17333	T17315	conj	remained,was
R4913	T17334	T17332	amod	general,in
R4914	T17335	T17333	punct	", ",remained
R4915	T17336	T17337	amod	mammalian,organogenesis
R4916	T17337	T17333	nsubj	organogenesis,remained
R4917	T17338	T17333	acomp	intact,remained
R4918	T17339	T17333	prep	in,remained
R4919	T17340	T17341	compound	Pygopus,mutants
R492	T2741	T2739	pobj	mesenchyme,by
R4920	T17341	T17339	pobj	mutants,in
R4921	T17342	T17333	punct	.,remained
R4922	T17344	T17345	advmod	Perhaps,is
R4923	T17346	T17347	det	the,explanation
R4924	T17347	T17345	nsubj	explanation,is
R4925	T17348	T17347	amod	simplest,explanation
R4926	T17349	T17350	mark	that,show
R4927	T17350	T17345	ccomp	show,is
R4928	T17351	T17350	prep	in,show
R4929	T17352	T17351	pobj	mammals,in
R493	T2742	T2741	amod	metanephric,mesenchyme
R4930	T17353	T17350	punct	", ",show
R4931	T17354	T17355	amod	other,genes
R4932	T17355	T17350	nsubj	genes,show
R4933	T17356	T17357	amod	partial,redundancy
R4934	T17357	T17350	dobj	redundancy,show
R4935	T17358	T17357	amod	functional,redundancy
R4936	T17359	T17357	prep	with,redundancy
R4937	T17360	T17361	det	the,genes
R4938	T17361	T17359	pobj	genes,with
R4939	T17362	T17361	nummod	two,genes
R494	T2743	T2744	punct	[,4
R4940	T17363	T17361	compound	Pygopus,genes
R4941	T17364	T17345	punct	.,is
R4942	T17366	T17367	det	The,complex
R4943	T17367	T17374	nsubj	complex,includes
R4944	T17368	T17369	compound	β,catenin
R4945	T17369	T17367	compound	catenin,complex
R4946	T17370	T17369	punct	-,catenin
R4947	T17371	T17372	compound	transcription,factor
R4948	T17372	T17367	compound	factor,complex
R4949	T17373	T17372	punct	-,factor
R495	T2744	T2711	parataxis	4,participates
R4950	T17375	T17376	det	a,number
R4951	T17376	T17374	dobj	number,includes
R4952	T17377	T17376	amod	large,number
R4953	T17378	T17377	cc	and,large
R4954	T17379	T17377	conj	growing,large
R4955	T17380	T17376	prep	of,number
R4956	T17381	T17380	pobj	proteins,of
R4957	T17382	T17381	punct	", ",proteins
R4958	T17383	T17384	dep	some,share
R4959	T17384	T17381	relcl	share,proteins
R496	T2745	T2744	punct	],4
R4960	T17385	T17383	prep	of,some
R4961	T17386	T17385	pobj	which,of
R4962	T17387	T17384	aux	may,share
R4963	T17388	T17389	det	the,functions
R4964	T17389	T17384	dobj	functions,share
R4965	T17390	T17391	amod	nuclear,localization
R4966	T17391	T17389	nmod	localization,functions
R4967	T17392	T17391	cc	and,localization
R4968	T17393	T17392	punct	/,and
R4969	T17394	T17392	cc	or,and
R497	T2746	T2711	punct	.,participates
R4970	T17395	T17396	compound	transcription,activation
R4971	T17396	T17391	conj	activation,localization
R4972	T17397	T17389	prep	of,functions
R4973	T17398	T17399	compound	Pygo1,Pygo2
R4974	T17399	T17397	pobj	Pygo2,of
R4975	T17400	T17399	punct	/,Pygo2
R4976	T17401	T17389	prep	in,functions
R4977	T17402	T17401	pobj	mammals,in
R4978	T17403	T17374	punct	.,includes
R4979	T17405	T17406	det	The,identities
R498	T2748	T2749	nsubj	Mutations,result
R4980	T17406	T17407	nsubj	identities,remain
R4981	T17408	T17406	cc	and,identities
R4982	T17409	T17406	conj	roles,identities
R4983	T17410	T17406	prep	of,identities
R4984	T17411	T17412	det	these,genes
R4985	T17412	T17410	pobj	genes,of
R4986	T17413	T17414	npadvmod	Pygopus,redundant
R4987	T17414	T17412	amod	redundant,genes
R4988	T17415	T17416	aux	to,determined
R4989	T17416	T17407	xcomp	determined,remain
R499	T2750	T2748	prep	in,Mutations
R4990	T17417	T17416	auxpass	be,determined
R4991	T17418	T17407	punct	.,remain
R4994	T18190	T18189	prep	of,Targeting
R4995	T18191	T18190	pobj	Pygo1,of
R4996	T18192	T18191	cc	and,Pygo1
R4997	T18193	T18191	conj	Pygo2,Pygo1
R4998	T18195	T18196	nmod	Pygo1,genes
R4999	T18196	T18199	nsubjpass	genes,targeted
R50	T996	T988	prep	in,functions
R500	T2751	T2750	pobj	Wnt9b,in
R5000	T18197	T18195	cc	and,Pygo1
R5001	T18198	T18195	conj	Pygo2,Pygo1
R5002	T18200	T18199	auxpass	were,targeted
R5003	T18201	T18199	advmod	each,targeted
R5004	T18202	T18199	prep	by,targeted
R5005	T18203	T18202	pcomp	flanking,by
R5006	T18204	T18205	det	the,regions
R5007	T18205	T18203	dobj	regions,flanking
R5008	T18206	T18205	amod	critical,regions
R5009	T18207	T18205	compound	coding,regions
R501	T2752	T2751	cc	or,Wnt9b
R5010	T18208	T18205	prep	of,regions
R5011	T18209	T18210	det	the,exon
R5012	T18210	T18208	pobj	exon,of
R5013	T18211	T18210	amod	third,exon
R5014	T18212	T18205	acl	containing,regions
R5015	T18213	T18214	det	the,motif
R5016	T18214	T18212	dobj	motif,containing
R5017	T18215	T18214	compound	PHD,motif
R5018	T18216	T18214	prep	with,motif
R5019	T18217	T18218	compound	LoxP,sequences
R502	T2753	T2751	conj	Wnt4,Wnt9b
R5020	T18218	T18216	pobj	sequences,with
R5021	T18219	T18199	punct	.,targeted
R5022	T18221	T18222	compound	Targeting,constructs
R5023	T18222	T18223	nsubjpass	constructs,made
R5024	T18224	T18223	auxpass	were,made
R5025	T18225	T18223	advcl	using,made
R5026	T18226	T18227	det	the,vector
R5027	T18227	T18225	dobj	vector,using
R5028	T18228	T18229	compound	Flp,Cre
R5029	T18229	T18227	compound	Cre,vector
R503	T2754	T2749	prep	in,result
R5030	T18230	T18229	punct	/,Cre
R5031	T18231	T18232	advmod	previously,described
R5032	T18232	T18227	acl	described,vector
R5033	T18233	T18234	punct	[,34
R5034	T18234	T18232	parataxis	34,described
R5035	T18235	T18234	punct	],34
R5036	T18236	T18227	punct	", ",vector
R5037	T18237	T18238	dep	which,carries
R5038	T18238	T18227	relcl	carries,vector
R5039	T18239	T18240	det	a,gene
R504	T2755	T2756	det	a,block
R5040	T18240	T18238	dobj	gene,carries
R5041	T18241	T18242	compound	neomycin,resistance
R5042	T18242	T18240	compound	resistance,gene
R5043	T18243	T18242	punct	-,resistance
R5044	T18244	T18240	acl	flanked,gene
R5045	T18245	T18244	agent	by,flanked
R5046	T18246	T18247	compound	FRT,sequences
R5047	T18247	T18245	pobj	sequences,by
R5048	T18248	T18227	punct	", ",vector
R5049	T18249	T18227	cc	and,vector
R505	T2756	T2754	pobj	block,in
R5050	T18250	T18251	nummod	three,sites
R5051	T18251	T18227	conj	sites,vector
R5052	T18252	T18251	amod	unique,sites
R5053	T18253	T18251	compound	restriction,sites
R5054	T18254	T18251	prep	for,sites
R5055	T18255	T18254	pcomp	subcloning,for
R5056	T18256	T18257	det	the,blocks
R5057	T18257	T18255	dobj	blocks,subcloning
R5058	T18258	T18257	nummod	two,blocks
R5059	T18259	T18257	prep	of,blocks
R506	T2757	T2756	amod	dramatic,block
R5060	T18260	T18259	pobj	homology,of
R5061	T18261	T18255	prep	for,subcloning
R5062	T18262	T18263	amod	driving,recombination
R5063	T18263	T18261	pobj	recombination,for
R5064	T18264	T18251	punct	", ",sites
R5065	T18265	T18251	cc	and,sites
R5066	T18266	T18267	det	the,region
R5067	T18267	T18251	conj	region,sites
R5068	T18268	T18267	amod	critical,region
R5069	T18269	T18270	aux	to,flanked
R507	T2758	T2756	prep	in,block
R5070	T18270	T18267	advcl	flanked,region
R5071	T18271	T18270	auxpass	be,flanked
R5072	T18272	T18270	agent	by,flanked
R5073	T18273	T18274	compound	LoxP,sequences
R5074	T18274	T18272	pobj	sequences,by
R5075	T18275	T18223	punct	.,made
R5076	T18277	T18278	prep	For,made
R5077	T18279	T18280	det	each,construct
R5078	T18280	T18277	pobj	construct,For
R5079	T18281	T18278	punct	", ",made
R508	T2759	T2760	compound	nephron,formation
R5080	T18282	T18283	det	the,segments
R5081	T18283	T18278	nsubjpass	segments,made
R5082	T18284	T18283	nummod	three,segments
R5083	T18285	T18283	amod	genomic,segments
R5084	T18286	T18283	acl	required,segments
R5085	T18287	T18286	prep	for,required
R5086	T18288	T18287	pobj	subcloning,for
R5087	T18289	T18278	auxpass	were,made
R5088	T18290	T18278	prep	by,made
R5089	T18291	T18290	pobj	PCR,by
R509	T2760	T2758	pobj	formation,in
R5090	T18292	T18291	prep	from,PCR
R5091	T18293	T18294	compound	RI,DNA
R5092	T18294	T18292	pobj	DNA,from
R5093	T18295	T18296	compound	ES,cell
R5094	T18296	T18294	compound	cell,DNA
R5095	T18297	T18278	punct	.,made
R5096	T18299	T18300	det	The,constructs
R5097	T18300	T18303	nsubjpass	constructs,confirmed
R5098	T18301	T18300	amod	resulting,constructs
R5099	T18302	T18300	compound	targeting,constructs
R51	T997	T998	compound	kidney,development
R510	T2761	T2749	punct	", ",result
R5100	T18304	T18303	auxpass	were,confirmed
R5101	T18305	T18303	prep	by,confirmed
R5102	T18306	T18305	pobj	sequencing,by
R5103	T18307	T18303	punct	.,confirmed
R5104	T18309	T18310	prep	For,were
R5105	T18311	T18309	pobj	Pygo1,For
R5106	T18312	T18310	punct	", ",were
R5107	T18313	T18314	det	the,sequences
R5108	T18314	T18310	nsubj	sequences,were
R5109	T18315	T18314	amod	genomic,sequences
R511	T2762	T2763	mark	while,show
R5110	T18316	T18314	acl	used,sequences
R5111	T18317	T18316	prep	for,used
R5112	T18318	T18317	pobj	PCR,for
R5113	T18319	T18310	punct	: ,were
R5114	T18320	T18321	npadvmod	5,forward
R5115	T18321	T18323	amod	forward,GTGAAGGAGAGATGGATAAGTATG
R5116	T18322	T18320	punct	',5
R5117	T18323	T18310	attr	GTGAAGGAGAGATGGATAAGTATG,were
R5118	T18324	T18323	punct	", ",GTGAAGGAGAGATGGATAAGTATG
R5119	T18325	T18323	punct	;,GTGAAGGAGAGATGGATAAGTATG
R512	T2763	T2749	advcl	show,result
R5120	T18326	T18327	npadvmod	5,back
R5121	T18327	T18329	amod	back,TAGACCCTAACCACCTACAAG
R5122	T18328	T18326	punct	',5
R5123	T18329	T18323	appos	TAGACCCTAACCACCTACAAG,GTGAAGGAGAGATGGATAAGTATG
R5124	T18330	T18329	punct	", ",TAGACCCTAACCACCTACAAG
R5125	T18331	T18323	punct	;,GTGAAGGAGAGATGGATAAGTATG
R5126	T18332	T18333	npadvmod	exon,forward
R5127	T18333	T18334	amod	forward,GGTTAGGGTCTATGTGCTGG
R5128	T18334	T18323	appos	GGTTAGGGTCTATGTGCTGG,GTGAAGGAGAGATGGATAAGTATG
R5129	T18335	T18334	punct	", ",GGTTAGGGTCTATGTGCTGG
R513	T2764	T2765	compound	Wnt11,mutants
R5130	T18336	T18323	punct	;,GTGAAGGAGAGATGGATAAGTATG
R5131	T18337	T18338	npadvmod	exon,back
R5132	T18338	T18339	amod	back,TCACCAAATCTCTGTTCTACAC
R5133	T18339	T18323	appos	TCACCAAATCTCTGTTCTACAC,GTGAAGGAGAGATGGATAAGTATG
R5134	T18340	T18339	punct	", ",TCACCAAATCTCTGTTCTACAC
R5135	T18341	T18323	punct	;,GTGAAGGAGAGATGGATAAGTATG
R5136	T18342	T18343	npadvmod	3,forward
R5137	T18343	T18345	amod	forward,TGTGTAGAACAGAGATTTGGTG
R5138	T18344	T18342	punct	',3
R5139	T18345	T18323	appos	TGTGTAGAACAGAGATTTGGTG,GTGAAGGAGAGATGGATAAGTATG
R514	T2765	T2763	nsubj	mutants,show
R5140	T18346	T18345	punct	", ",TGTGTAGAACAGAGATTTGGTG
R5141	T18347	T18323	punct	;,GTGAAGGAGAGATGGATAAGTATG
R5142	T18348	T18349	npadvmod	3,back
R5143	T18349	T18351	amod	back,CAGTGAAGAAAGAGGGTCAG
R5144	T18350	T18348	punct	',3
R5145	T18351	T18323	appos	CAGTGAAGAAAGAGGGTCAG,GTGAAGGAGAGATGGATAAGTATG
R5146	T18352	T18351	punct	", ",CAGTGAAGAAAGAGGGTCAG
R5147	T18353	T18310	punct	.,were
R5148	T18355	T18356	prep	For,were
R5149	T18357	T18355	pobj	Pygo2,For
R515	T2766	T2767	det	a,reduction
R5150	T18358	T18356	punct	", ",were
R5151	T18359	T18360	det	the,sequences
R5152	T18360	T18356	nsubj	sequences,were
R5153	T18361	T18360	amod	genomic,sequences
R5154	T18362	T18360	acl	used,sequences
R5155	T18363	T18362	prep	for,used
R5156	T18364	T18363	pobj	PCR,for
R5157	T18365	T18356	punct	: ,were
R5158	T18366	T18356	attr	GCCTGGGTTGCTTGTCTTCTG,were
R5159	T18367	T18366	cc	and,GCCTGGGTTGCTTGTCTTCTG
R516	T2767	T2763	dobj	reduction,show
R5160	T18368	T18366	conj	CCACCTTACTTGTGTGTGAGGATACATAC,GCCTGGGTTGCTTGTCTTCTG
R5161	T18369	T18366	punct	", ",GCCTGGGTTGCTTGTCTTCTG
R5162	T18370	T18366	conj	CCAAGTCCCAGCATCTCTTAC,GCCTGGGTTGCTTGTCTTCTG
R5163	T18371	T18370	cc	and,CCAAGTCCCAGCATCTCTTAC
R5164	T18372	T18370	conj	CCAGTCATACCAGCAACAAG,CCAAGTCCCAGCATCTCTTAC
R5165	T18373	T18370	punct	", ",CCAAGTCCCAGCATCTCTTAC
R5166	T18374	T18370	cc	and,CCAAGTCCCAGCATCTCTTAC
R5167	T18375	T18376	compound	exon,sequences
R5168	T18376	T18377	compound	sequences,TGGGTGCTGGGAACAGAAC
R5169	T18377	T18370	conj	TGGGTGCTGGGAACAGAAC,CCAAGTCCCAGCATCTCTTAC
R517	T2768	T2767	amod	significant,reduction
R5170	T18378	T18377	cc	and,TGGGTGCTGGGAACAGAAC
R5171	T18379	T18377	conj	CAACAACAACAGAAGACAAGC,TGGGTGCTGGGAACAGAAC
R5172	T18380	T18356	punct	.,were
R5173	T18382	T18383	amod	Linearized,constructs
R5174	T18383	T18384	nsubjpass	constructs,electroporated
R5175	T18385	T18384	auxpass	were,electroporated
R5176	T18386	T18384	prep	into,electroporated
R5177	T18387	T18388	compound	RI,cells
R5178	T18388	T18386	pobj	cells,into
R5179	T18389	T18388	compound	ES,cells
R518	T2769	T2767	prep	in,reduction
R5180	T18390	T18384	punct	", ",electroporated
R5181	T18391	T18384	cc	and,electroporated
R5182	T18392	T18393	amod	resulting,cells
R5183	T18393	T18396	nsubj	cells,used
R5184	T18394	T18393	amod	targeted,cells
R5185	T18395	T18393	compound	ES,cells
R5186	T18396	T18384	conj	used,electroporated
R5187	T18397	T18396	prep	for,used
R5188	T18398	T18399	compound	C57,Bl6
R5189	T18399	T18401	compound	Bl6,injections
R519	T2770	T2771	compound	nephron,number
R5190	T18400	T18399	punct	/,Bl6
R5191	T18401	T18397	pobj	injections,for
R5192	T18402	T18401	compound	blastocyst,injections
R5193	T18403	T18396	prep	according,used
R5194	T18404	T18403	prep	to,according
R5195	T18405	T18406	amod	standard,protocols
R5196	T18406	T18404	pobj	protocols,to
R5197	T18407	T18396	punct	.,used
R5198	T18409	T18410	amod	Resulting,chimeras
R5199	T18410	T18411	nsubjpass	chimeras,mated
R52	T998	T996	pobj	development,in
R520	T2771	T2769	pobj	number,in
R5200	T18412	T18411	auxpass	were,mated
R5201	T18413	T18411	prep	with,mated
R5202	T18414	T18415	compound	Swiss,Black
R5203	T18415	T18416	compound	Black,mice
R5204	T18416	T18413	pobj	mice,with
R5205	T18417	T18411	punct	", ",mated
R5206	T18418	T18411	cc	and,mated
R5207	T18419	T18420	det	the,stocks
R5208	T18420	T18422	nsubj	stocks,maintained
R5209	T18421	T18420	amod	targeted,stocks
R521	T2772	T2749	punct	.,result
R5210	T18422	T18411	conj	maintained,mated
R5211	T18423	T18422	prep	on,maintained
R5212	T18424	T18425	det	a,background
R5213	T18425	T18423	pobj	background,on
R5214	T18426	T18425	amod	mixed,background
R5215	T18427	T18428	nummod	129,Black
R5216	T18428	T18425	compound	Black,background
R5217	T18429	T18428	punct	/,Black
R5218	T18430	T18428	compound	Swiss,Black
R5219	T18431	T18422	punct	.,maintained
R522	T2774	T2775	nsubj	It,is
R5220	T18433	T18434	nmod	Germline,alleles
R5221	T18434	T18436	nsubjpass	alleles,generated
R5222	T18435	T18434	amod	null,alleles
R5223	T18437	T18434	prep	of,alleles
R5224	T18438	T18439	preconj	both,Pygo1
R5225	T18439	T18437	pobj	Pygo1,of
R5226	T18440	T18439	cc	and,Pygo1
R5227	T18441	T18439	conj	Pygo2,Pygo1
R5228	T18442	T18436	auxpass	were,generated
R5229	T18443	T18436	prep	by,generated
R523	T2776	T2775	acomp	interesting,is
R5230	T18444	T18443	pcomp	mating,by
R5231	T18445	T18446	amod	heterozygous,mice
R5232	T18446	T18444	dobj	mice,mating
R5233	T18447	T18446	amod	floxed,mice
R5234	T18448	T18444	prep	with,mating
R5235	T18449	T18450	det	the,mice
R5236	T18450	T18448	pobj	mice,with
R5237	T18451	T18452	compound	CMV,Cre
R5238	T18452	T18450	compound	Cre,mice
R5239	T18453	T18452	punct	-,Cre
R524	T2777	T2778	aux	to,note
R5240	T18454	T18455	punct	[,15
R5241	T18455	T18436	parataxis	15,generated
R5242	T18456	T18455	punct	],15
R5243	T18457	T18436	punct	.,generated
R5244	T18459	T18460	det	The,sequences
R5245	T18460	T18461	nsubj	sequences,were
R5246	T18462	T18460	prep	of,sequences
R5247	T18463	T18464	det	the,primers
R5248	T18464	T18462	pobj	primers,of
R5249	T18465	T18464	acl	used,primers
R525	T2778	T2775	xcomp	note,is
R5250	T18466	T18465	prep	for,used
R5251	T18467	T18468	amod	genotyping,PCR
R5252	T18468	T18466	pobj	PCR,for
R5253	T18469	T18461	punct	: ,were
R5254	T18470	T18471	npadvmod	Pygo2,null
R5255	T18471	T18472	amod	null,allele
R5256	T18472	T18461	attr	allele,were
R5257	T18473	T18472	punct	", ",allele
R5258	T18474	T18475	nmod	forward,CCTGGATTCTTGTTGCTGGTATG
R5259	T18475	T18472	appos	CCTGGATTCTTGTTGCTGGTATG,allele
R526	T2779	T2780	mark	that,shown
R5260	T18476	T18474	punct	(,forward
R5261	T18477	T18474	appos	F,forward
R5262	T18478	T18475	punct	),CCTGGATTCTTGTTGCTGGTATG
R5263	T18479	T18475	punct	;,CCTGGATTCTTGTTGCTGGTATG
R5264	T18480	T18481	nmod	reverse,AAGGTATTTGGTGCTCCGAGGG
R5265	T18481	T18475	appos	AAGGTATTTGGTGCTCCGAGGG,CCTGGATTCTTGTTGCTGGTATG
R5266	T18482	T18480	punct	(,reverse
R5267	T18483	T18480	appos	R,reverse
R5268	T18484	T18481	punct	),AAGGTATTTGGTGCTCCGAGGG
R5269	T18485	T18472	punct	;,allele
R527	T2780	T2778	ccomp	shown,note
R5270	T18486	T18487	nmod	Pygo2,allele
R5271	T18487	T18472	appos	allele,allele
R5272	T18488	T18487	nmod	WT,allele
R5273	T18489	T18488	cc	or,WT
R5274	T18490	T18488	conj	floxed,WT
R5275	T18491	T18487	punct	", ",allele
R5276	T18492	T18493	compound	F,TGTCTTGATGACAGCGTTTAGCC
R5277	T18493	T18487	appos	TGTCTTGATGACAGCGTTTAGCC,allele
R5278	T18494	T18493	punct	", ",TGTCTTGATGACAGCGTTTAGCC
R5279	T18495	T18496	compound	R,AGATTCAGTAAGCTGAGCCTGGTG
R528	T2781	T2780	nsubjpass	Wnt4,shown
R5280	T18496	T18493	appos	AGATTCAGTAAGCTGAGCCTGGTG,TGTCTTGATGACAGCGTTTAGCC
R5281	T18497	T18472	punct	;,allele
R5282	T18498	T18499	npadvmod	Pygo1,null
R5283	T18499	T18500	amod	null,allele
R5284	T18500	T18472	appos	allele,allele
R5285	T18501	T18500	punct	", ",allele
R5286	T18502	T18503	compound	F,AGTTTGAAATAGCGACGAGTTTGAG
R5287	T18503	T18500	appos	AGTTTGAAATAGCGACGAGTTTGAG,allele
R5288	T18504	T18503	punct	", ",AGTTTGAAATAGCGACGAGTTTGAG
R5289	T18505	T18506	nmod	R,5
R529	T2782	T2781	cc	and,Wnt4
R5290	T18506	T18507	nummod	5,CACTTCTGCCCCTCTCTTTGC
R5291	T18507	T18503	appos	CACTTCTGCCCCTCTCTTTGC,AGTTTGAAATAGCGACGAGTTTGAG
R5292	T18508	T18506	punct	',5
R5293	T18509	T18507	punct	-,CACTTCTGCCCCTCTCTTTGC
R5294	T18510	T18472	punct	;,allele
R5295	T18511	T18512	nmod	Pygo1,allele
R5296	T18512	T18472	appos	allele,allele
R5297	T18513	T18512	nmod	WT,allele
R5298	T18514	T18513	cc	or,WT
R5299	T18515	T18513	conj	floxed,WT
R53	T999	T986	punct	", ",made
R530	T2783	T2781	conj	Wnt11,Wnt4
R5300	T18516	T18512	punct	", ",allele
R5301	T18517	T18518	compound	F,AAGCGTGCCTCATCTCCATCCCTAAG
R5302	T18518	T18512	appos	AAGCGTGCCTCATCTCCATCCCTAAG,allele
R5303	T18519	T18518	punct	", ",AAGCGTGCCTCATCTCCATCCCTAAG
R5304	T18520	T18521	compound	R,GCCCTCCCCGACGTTTATATTG
R5305	T18521	T18518	appos	GCCCTCCCCGACGTTTATATTG,AAGCGTGCCTCATCTCCATCCCTAAG
R5306	T18522	T18461	punct	.,were
R5307	T18524	T18525	det	The,noon
R5308	T18525	T18526	nsubjpass	noon,designated
R5309	T18527	T18525	prep	of,noon
R531	T2784	T2780	aux	have,shown
R5310	T18528	T18529	det	the,day
R5311	T18529	T18527	pobj	day,of
R5312	T18530	T18531	prep	that,observed
R5313	T18531	T18529	relcl	observed,day
R5314	T18532	T18533	amod	vaginal,plugs
R5315	T18533	T18531	nsubjpass	plugs,observed
R5316	T18534	T18531	auxpass	were,observed
R5317	T18535	T18526	auxpass	was,designated
R5318	T18536	T18526	oprd	E0.5,designated
R5319	T18537	T18526	punct	.,designated
R532	T2785	T2780	auxpass	been,shown
R5320	T19104	T19105	amod	Confocal,microscopy
R5321	T19107	T19108	nsubj	Kidneys,dissected
R5322	T19109	T19108	aux	were,dissected
R5323	T19110	T19108	punct	", ",dissected
R5324	T19111	T19108	conj	fixed,dissected
R5325	T19112	T19111	prep	in,fixed
R5326	T19113	T19112	pobj	paraformaldehyde,in
R5327	T19114	T19111	punct	", ",fixed
R5328	T19115	T19111	conj	treated,fixed
R5329	T19116	T19115	prep	with,treated
R533	T2786	T2787	aux	to,signal
R5330	T19117	T19116	pobj	methanol,with
R5331	T19118	T19115	punct	", ",treated
R5332	T19119	T19115	cc	and,treated
R5333	T19120	T19115	conj	washed,treated
R5334	T19121	T19120	prep	with,washed
R5335	T19122	T19121	pobj	PBS,with
R5336	T19123	T19122	acl	containing,PBS
R5337	T19124	T19123	dobj	Tween,containing
R5338	T19125	T19124	punct	-,Tween
R5339	T19126	T19124	nummod	20,Tween
R534	T2787	T2780	xcomp	signal,shown
R5340	T19127	T19122	punct	(,PBS
R5341	T19128	T19129	compound	PBS,T
R5342	T19129	T19122	appos	T,PBS
R5343	T19130	T19129	punct	-,T
R5344	T19131	T19108	punct	),dissected
R5345	T19132	T19133	amod	prior,to
R5346	T19133	T19108	prep	to,dissected
R5347	T19134	T19133	pobj	treatment,to
R5348	T19135	T19134	prep	with,treatment
R5349	T19136	T19135	pobj	lectins,with
R535	T2788	T2787	punct	", ",signal
R5350	T19137	T19136	cc	and,lectins
R5351	T19138	T19136	conj	antibodies,lectins
R5352	T19139	T19108	punct	.,dissected
R5353	T19141	T19142	compound	PBS,T
R5354	T19142	T19144	nsubjpass	T,used
R5355	T19143	T19142	punct	-,T
R5356	T19145	T19144	auxpass	was,used
R5357	T19146	T19144	prep	for,used
R5358	T19147	T19146	pobj	incubations,for
R5359	T19148	T19147	prep	of,incubations
R536	T2789	T2790	advmod	at,least
R5360	T19149	T19150	det	the,tissues
R5361	T19150	T19148	pobj	tissues,of
R5362	T19151	T19147	prep	with,incubations
R5363	T19152	T19153	npadvmod	fluoroscein,conjugated
R5364	T19153	T19155	amod	conjugated,aggutinin
R5365	T19154	T19153	punct	-,conjugated
R5366	T19155	T19151	pobj	aggutinin,with
R5367	T19156	T19155	compound	Dilichos,aggutinin
R5368	T19157	T19155	compound	biflorus,aggutinin
R5369	T19158	T19155	punct	(,aggutinin
R537	T2790	T2791	advmod	least,in
R5370	T19159	T19155	appos	DBA,aggutinin
R5371	T19160	T19155	punct	", ",aggutinin
R5372	T19161	T19155	parataxis	Vector,aggutinin
R5373	T19162	T19161	punct	;,Vector
R5374	T19163	T19161	npadvmod	Burlingame,Vector
R5375	T19164	T19161	punct	", ",Vector
R5376	T19165	T19161	npadvmod	USA,Vector
R5377	T19166	T19161	punct	),Vector
R5378	T19167	T19144	punct	", ",used
R5379	T19168	T19144	cc	and,used
R538	T2791	T2787	prep	in,signal
R5380	T19169	T19170	compound	PBS,T
R5381	T19170	T19172	nsubjpass	T,used
R5382	T19171	T19170	punct	-,T
R5383	T19172	T19144	conj	used,used
R5384	T19173	T19170	cc	plus,T
R5385	T19174	T19175	nummod	2,%
R5386	T19175	T19176	compound	%,serum
R5387	T19176	T19170	conj	serum,T
R5388	T19177	T19176	compound	goat,serum
R5389	T19178	T19172	auxpass	was,used
R539	T2792	T2793	det	some,cases
R5390	T19179	T19172	prep	for,used
R5391	T19180	T19179	pobj	incubations,for
R5392	T19181	T19180	prep	with,incubations
R5393	T19182	T19183	det	the,antibodies
R5394	T19183	T19181	pobj	antibodies,with
R5395	T19184	T19172	punct	.,used
R5396	T19186	T19187	det	The,antibodies
R5397	T19187	T19189	nsubj	antibodies,were
R5398	T19188	T19187	amod	primary,antibodies
R5399	T19190	T19189	attr	anti-WT1,were
R54	T1000	T1001	amod	targeted,mutations
R540	T2793	T2791	pobj	cases,in
R5400	T19191	T19192	punct	(,Biotechnology
R5401	T19192	T19190	parataxis	Biotechnology,anti-WT1
R5402	T19193	T19192	dep	c,Biotechnology
R5403	T19194	T19193	punct	-,c
R5404	T19195	T19193	nummod	19,c
R5405	T19196	T19192	punct	;,Biotechnology
R5406	T19197	T19198	compound	Santa,Cruz
R5407	T19198	T19192	compound	Cruz,Biotechnology
R5408	T19199	T19192	punct	", ",Biotechnology
R5409	T19200	T19201	compound	Santa,Cruz
R541	T2794	T2787	punct	", ",signal
R5410	T19201	T19192	npadvmod	Cruz,Biotechnology
R5411	T19202	T19192	punct	", ",Biotechnology
R5412	T19203	T19192	npadvmod	CA,Biotechnology
R5413	T19204	T19192	punct	", ",Biotechnology
R5414	T19205	T19192	npadvmod	USA,Biotechnology
R5415	T19206	T19192	punct	),Biotechnology
R5416	T19207	T19190	punct	", ",anti-WT1
R5417	T19208	T19190	conj	anti-uvomorulin,anti-WT1
R5418	T19209	T19208	punct	(,anti-uvomorulin
R5419	T19210	T19211	compound	e,cadherin
R542	T2795	T2787	prep	through,signal
R5420	T19211	T19208	appos	cadherin,anti-uvomorulin
R5421	T19212	T19211	punct	-,cadherin
R5422	T19213	T19208	punct	", ",anti-uvomorulin
R5423	T19214	T19208	appos	Cdh1,anti-uvomorulin
R5424	T19215	T19216	punct	", ",Aldrich
R5425	T19216	T19208	parataxis	Aldrich,anti-uvomorulin
R5426	T19217	T19216	compound	Sigma,Aldrich
R5427	T19218	T19216	punct	-,Aldrich
R5428	T19219	T19220	compound	St.,Louis
R5429	T19220	T19216	npadvmod	Louis,Aldrich
R543	T2796	T2797	amod	noncanonical,pathways
R5430	T19221	T19220	punct	", ",Louis
R5431	T19222	T19216	npadvmod	MO,Aldrich
R5432	T19223	T19216	punct	", ",Aldrich
R5433	T19224	T19216	npadvmod	USA,Aldrich
R5434	T19225	T19216	punct	),Aldrich
R5435	T19226	T19208	punct	", ",anti-uvomorulin
R5436	T19227	T19208	cc	and,anti-uvomorulin
R5437	T19228	T19208	conj	anti-Cited1,anti-uvomorulin
R5438	T19229	T19230	punct	(,Corporation
R5439	T19230	T19228	parataxis	Corporation,anti-Cited1
R544	T2797	T2795	pobj	pathways,through
R5440	T19231	T19230	compound	Neomarkers,Corporation
R5441	T19232	T19230	punct	-,Corporation
R5442	T19233	T19230	compound	Lab,Corporation
R5443	T19234	T19230	compound	Vision,Corporation
R5444	T19235	T19230	punct	", ",Corporation
R5445	T19236	T19230	npadvmod	Fremont,Corporation
R5446	T19237	T19230	punct	", ",Corporation
R5447	T19238	T19230	npadvmod	CA,Corporation
R5448	T19239	T19230	punct	", ",Corporation
R5449	T19240	T19230	npadvmod	USA,Corporation
R545	T2798	T2799	punct	[,5
R5450	T19241	T19230	punct	),Corporation
R5451	T19242	T19189	punct	.,were
R5452	T19244	T19245	det	The,antibodies
R5453	T19245	T19247	nsubj	antibodies,were
R5454	T19246	T19245	amod	secondary,antibodies
R5455	T19248	T19249	npadvmod	Alexa,conjugated
R5456	T19249	T19252	amod	conjugated,anti-rabbit
R5457	T19250	T19248	nummod	555,Alexa
R5458	T19251	T19249	punct	-,conjugated
R5459	T19252	T19253	nmod	anti-rabbit,antibodies
R546	T2799	T2787	parataxis	5,signal
R5460	T19253	T19247	attr	antibodies,were
R5461	T19254	T19252	cc	and,anti-rabbit
R5462	T19255	T19256	npadvmod	Alexa,conjugated
R5463	T19256	T19259	amod	conjugated,anti-rat
R5464	T19257	T19255	nummod	633,Alexa
R5465	T19258	T19256	punct	-,conjugated
R5466	T19259	T19252	conj	anti-rat,anti-rabbit
R5467	T19260	T19261	punct	(,Probes
R5468	T19261	T19253	parataxis	Probes,antibodies
R5469	T19262	T19261	compound	Molecular,Probes
R547	T2800	T2801	punct	-,7
R5470	T19263	T19261	punct	", ",Probes
R5471	T19264	T19261	npadvmod	Eugene,Probes
R5472	T19265	T19261	punct	", ",Probes
R5473	T19266	T19261	npadvmod	OR,Probes
R5474	T19267	T19261	punct	", ",Probes
R5475	T19268	T19261	npadvmod	USA,Probes
R5476	T19269	T19261	punct	),Probes
R5477	T19270	T19247	punct	.,were
R5478	T19272	T19273	det	The,tissues
R5479	T19273	T19274	nsubjpass	tissues,imaged
R548	T2801	T2799	prep	7,5
R5480	T19275	T19274	auxpass	were,imaged
R5481	T19276	T19274	prep	with,imaged
R5482	T19277	T19278	det	a,microscope
R5483	T19278	T19276	pobj	microscope,with
R5484	T19279	T19278	compound	laser,microscope
R5485	T19280	T19278	compound	scanning,microscope
R5486	T19281	T19282	punct	(,Zeiss
R5487	T19282	T19278	parataxis	Zeiss,microscope
R5488	T19283	T19282	compound	Carl,Zeiss
R5489	T19284	T19282	punct	", ",Zeiss
R549	T2802	T2799	punct	],5
R5490	T19285	T19282	npadvmod	Thornwood,Zeiss
R5491	T19286	T19282	punct	", ",Zeiss
R5492	T19287	T19288	compound	New,York
R5493	T19288	T19282	npadvmod	York,Zeiss
R5494	T19289	T19282	punct	", ",Zeiss
R5495	T19290	T19282	npadvmod	USA,Zeiss
R5496	T19291	T19282	punct	),Zeiss
R5497	T19292	T19278	acl	equipped,microscope
R5498	T19293	T19292	prep	with,equipped
R5499	T19294	T19295	nummod	an,argon
R55	T1001	T986	nsubjpass	mutations,made
R550	T2803	T2778	punct	", ",note
R5500	T19295	T19293	pobj	argon,with
R5501	T19296	T19297	punct	(,nm
R5502	T19297	T19295	parataxis	nm,argon
R5503	T19298	T19297	nummod	488,nm
R5504	T19299	T19297	punct	),nm
R5505	T19300	T19295	cc	and,argon
R5506	T19301	T19302	nummod	two,HeNe
R5507	T19302	T19303	compound	HeNe,lasers
R5508	T19303	T19295	conj	lasers,argon
R5509	T19304	T19305	punct	(,nm
R551	T2804	T2805	mark	while,is
R5510	T19305	T19303	parataxis	nm,lasers
R5511	T19306	T19305	nummod	543,nm
R5512	T19307	T19305	cc	and,nm
R5513	T19308	T19309	nummod	633,nm
R5514	T19309	T19305	conj	nm,nm
R5515	T19310	T19305	punct	),nm
R5516	T19311	T19274	punct	.,imaged
R5517	T19313	T19314	amod	Optical,sections
R5518	T19314	T19315	nsubjpass	sections,obtained
R5519	T19316	T19317	advmod	approximately,2
R552	T2805	T2778	advcl	is,note
R5520	T19317	T19318	nummod	2,μm
R5521	T19318	T19319	compound	μm,thick
R5522	T19319	T19314	amod	thick,sections
R5523	T19320	T19315	auxpass	were,obtained
R5524	T19321	T19322	det	every,μm
R5525	T19322	T19315	npadvmod	μm,obtained
R5526	T19323	T19322	nummod	5,μm
R5527	T19324	T19315	prep	to,obtained
R5528	T19325	T19326	det	a,depth
R5529	T19326	T19324	pobj	depth,to
R553	T2806	T2805	expl	there,is
R5530	T19327	T19326	prep	of,depth
R5531	T19328	T19329	advmod	at,65
R5532	T19329	T19331	nummod	65,μm
R5533	T19330	T19329	advmod	least,65
R5534	T19331	T19327	pobj	μm,of
R5535	T19332	T19315	punct	.,obtained
R5536	T19334	T19335	det	The,sections
R5537	T19335	T19336	nsubj	sections,began
R5538	T19337	T19336	prep	at,began
R5539	T19338	T19339	det	the,surface
R554	T2807	T2805	attr	evidence,is
R5540	T19339	T19337	pobj	surface,at
R5541	T19340	T19339	prep	of,surface
R5542	T19341	T19342	det	the,kidney
R5543	T19342	T19340	pobj	kidney,of
R5544	T19343	T19336	cc	and,began
R5545	T19344	T19336	conj	were,began
R5546	T19345	T19344	prep	on,were
R5547	T19346	T19347	det	a,plane
R5548	T19347	T19345	pobj	plane,on
R5549	T19348	T19347	amod	tangential,plane
R555	T2808	T2807	acl	indicating,evidence
R5550	T19349	T19348	prep	to,tangential
R5551	T19350	T19349	pobj	it,to
R5552	T19351	T19336	punct	.,began
R5553	T19353	T19354	nummod	Two,series
R5554	T19354	T19358	nsubjpass	series,obtained
R5555	T19355	T19356	compound	Z,stack
R5556	T19356	T19354	compound	stack,series
R5557	T19357	T19356	punct	-,stack
R5558	T19359	T19358	auxpass	were,obtained
R5559	T19360	T19358	punct	", ",obtained
R556	T2809	T2810	mark	that,activates
R5560	T19361	T19358	dobj	one,obtained
R5561	T19362	T19361	prep	from,one
R5562	T19363	T19362	pobj	each,from
R5563	T19364	T19363	prep	of,each
R5564	T19365	T19366	det	the,kidneys
R5565	T19366	T19364	pobj	kidneys,of
R5566	T19367	T19366	nummod	two,kidneys
R5567	T19368	T19366	prep	of,kidneys
R5568	T19369	T19370	det	each,embryo
R5569	T19370	T19368	pobj	embryo,of
R557	T2810	T2808	ccomp	activates,indicating
R5570	T19371	T19358	punct	.,obtained
R5571	T19373	T19374	amod	Ureteric,bud
R5572	T19374	T19375	compound	bud,tips
R5573	T19375	T19376	nsubjpass	tips,counted
R5574	T19377	T19375	acl	identified,tips
R5575	T19378	T19377	prep	by,identified
R5576	T19379	T19380	compound	section,tracing
R5577	T19380	T19378	pobj	tracing,by
R5578	T19381	T19376	auxpass	were,counted
R5579	T19382	T19376	prep	within,counted
R558	T2811	T2810	nsubj	Wnt9b,activates
R5580	T19383	T19384	det	a,area
R5581	T19384	T19382	pobj	area,within
R5582	T19385	T19384	amod	defined,area
R5583	T19386	T19384	prep	of,area
R5584	T19387	T19388	det	the,image
R5585	T19388	T19386	pobj	image,of
R5586	T19389	T19388	amod	confocal,image
R5587	T19390	T19376	punct	.,counted
R5588	T19486	T19487	advmod	In,situ
R5589	T19487	T19488	amod	situ,hybridization
R559	T2812	T2813	amod	canonical,signaling
R5590	T19490	T19491	amod	Whole,mount
R5591	T19491	T19493	nmod	mount,hybridization
R5592	T19492	T19491	punct	-,mount
R5593	T19493	T19496	nsubjpass	hybridization,performed
R5594	T19494	T19495	advmod	in,situ
R5595	T19495	T19493	amod	situ,hybridization
R5596	T19497	T19496	auxpass	was,performed
R5597	T19498	T19499	mark	as,described
R5598	T19499	T19496	advcl	described,performed
R5599	T19500	T19499	advmod	previously,described
R56	T1002	T986	auxpass	were,made
R560	T2813	T2810	dobj	signaling,activates
R5600	T19501	T19502	punct	[,35
R5601	T19502	T19499	parataxis	35,described
R5602	T19503	T19502	punct	],35
R5603	T19504	T19496	punct	.,performed
R5604	T19506	T19507	nsubjpass	Riboprobes,described
R5605	T19508	T19506	prep	to,Riboprobes
R5606	T19509	T19508	pobj	Wnt11,to
R5607	T19510	T19509	cc	and,Wnt11
R5608	T19511	T19509	conj	Wnt7b,Wnt11
R5609	T19512	T19507	auxpass	were,described
R561	T2814	T2813	compound	Wnt,signaling
R5610	T19513	T19507	advmod	previously,described
R5611	T19514	T19515	punct	[,35
R5612	T19515	T19507	parataxis	35,described
R5613	T19516	T19515	punct	],35
R5614	T19517	T19507	punct	.,described
R5615	T19519	T19520	det	The,riboprobe
R5616	T19520	T19522	nsubjpass	riboprobe,provided
R5617	T19521	T19520	compound	Wnt9b,riboprobe
R5618	T19523	T19522	auxpass	was,provided
R5619	T19524	T19522	agent	by,provided
R562	T2815	T2810	prep	in,activates
R5620	T19525	T19526	compound	T.,Carroll
R5621	T19526	T19524	pobj	Carroll,by
R5622	T19527	T19528	punct	[,2
R5623	T19528	T19522	parataxis	2,provided
R5624	T19529	T19528	punct	],2
R5625	T19530	T19522	punct	.,provided
R5626	T20069	T20070	nmod	Pygo1,antibody
R5627	T20070	T20073	compound	antibody,production
R5628	T20071	T20069	cc	and,Pygo1
R5629	T20072	T20069	conj	Pygo2,Pygo1
R563	T2816	T2817	det	the,kidney
R5630	T20075	T20076	aux	To,generate
R5631	T20076	T20077	advcl	generate,subcloned
R5632	T20078	T20079	amod	anti-human,Pygo2
R5633	T20079	T20080	nmod	Pygo2,antibodies
R5634	T20080	T20076	dobj	antibodies,generate
R5635	T20081	T20079	punct	(,Pygo2
R5636	T20082	T20079	appos	anti-hPygo2,Pygo2
R5637	T20083	T20079	punct	),Pygo2
R5638	T20084	T20079	cc	and,Pygo2
R5639	T20085	T20086	amod	anti-mouse,Pygo1
R564	T2817	T2815	pobj	kidney,in
R5640	T20086	T20079	conj	Pygo1,Pygo2
R5641	T20087	T20086	punct	(,Pygo1
R5642	T20088	T20086	appos	anti-mPygo1,Pygo1
R5643	T20089	T20080	punct	),antibodies
R5644	T20090	T20080	amod	polyclonal,antibodies
R5645	T20091	T20077	punct	", ",subcloned
R5646	T20092	T20077	nsubj	we,subcloned
R5647	T20093	T20077	dobj	cDNA,subcloned
R5648	T20094	T20077	prep	by,subcloned
R5649	T20095	T20094	pobj	PCR,by
R565	T2818	T2819	punct	[,2
R5650	T20096	T20095	acl	corresponding,PCR
R5651	T20097	T20096	prep	to,corresponding
R5652	T20098	T20099	nmod	amino,acid
R5653	T20099	T20101	nmod	acid,80
R5654	T20100	T20099	punct	-,acid
R5655	T20101	T20097	pobj	80,to
R5656	T20102	T20101	nmod	residues,80
R5657	T20103	T20104	punct	–,327
R5658	T20104	T20101	prep	327,80
R5659	T20105	T20101	prep	of,80
R566	T2819	T2810	parataxis	2,activates
R5660	T20106	T20107	amod	human,Pygo
R5661	T20107	T20105	pobj	Pygo,of
R5662	T20108	T20107	nummod	2,Pygo
R5663	T20109	T20101	cc	or,80
R5664	T20110	T20111	nmod	amino,acid
R5665	T20111	T20113	nmod	acid,76
R5666	T20112	T20111	punct	-,acid
R5667	T20113	T20101	conj	76,80
R5668	T20114	T20113	nmod	residues,76
R5669	T20115	T20116	punct	–,263
R567	T2820	T2819	punct	],2
R5670	T20116	T20113	prep	263,76
R5671	T20117	T20113	prep	of,76
R5672	T20118	T20119	compound	mouse,Pygo1
R5673	T20119	T20117	pobj	Pygo1,of
R5674	T20120	T20077	prep	into,subcloned
R5675	T20121	T20120	pobj	pGEX4T1,into
R5676	T20122	T20123	punct	(,Health
R5677	T20123	T20121	parataxis	Health,pGEX4T1
R5678	T20124	T20123	compound	Amersham,Health
R5679	T20125	T20123	punct	", ",Health
R568	T2821	T2775	punct	.,is
R5680	T20126	T20123	npadvmod	Piscataway,Health
R5681	T20127	T20123	punct	", ",Health
R5682	T20128	T20123	npadvmod	NJ,Health
R5683	T20129	T20123	punct	", ",Health
R5684	T20130	T20123	npadvmod	USA,Health
R5685	T20131	T20123	punct	),Health
R5686	T20132	T20077	punct	.,subcloned
R5687	T20134	T20135	det	The,fragments
R5688	T20135	T20137	nsubj	fragments,lack
R5689	T20136	T20135	compound	PCR,fragments
R569	T2823	T2824	amod	Genetic,studies
R5690	T20138	T20135	prep	of,fragments
R5691	T20139	T20138	pobj	hPygo,of
R5692	T20140	T20139	nummod	2,hPygo
R5693	T20141	T20139	cc	and,hPygo
R5694	T20142	T20139	conj	mPygo,hPygo
R5695	T20143	T20142	nummod	1,mPygo
R5696	T20144	T20145	preconj	both,NHD
R5697	T20145	T20146	npadvmod	NHD,conserved
R5698	T20146	T20149	amod	conserved,regions
R5699	T20147	T20145	cc	and,NHD
R57	T1003	T986	prep	in,made
R570	T2824	T2825	nsubj	studies,identified
R5700	T20148	T20145	conj	PHD,NHD
R5701	T20149	T20137	dobj	regions,lack
R5702	T20150	T20149	prep	of,regions
R5703	T20151	T20150	pobj	hPygo2,of
R5704	T20152	T20151	cc	and,hPygo2
R5705	T20153	T20151	conj	mPygo1,hPygo2
R5706	T20154	T20137	punct	.,lack
R5707	T20156	T20157	nmod	GST,hPygo2
R5708	T20157	T20159	nmod	hPygo2,proteins
R5709	T20158	T20157	punct	-,hPygo2
R571	T2826	T2824	prep	in,studies
R5710	T20159	T20164	nsubjpass	proteins,expressed
R5711	T20160	T20157	cc	and,hPygo2
R5712	T20161	T20162	compound	GST,mPygo1
R5713	T20162	T20157	conj	mPygo1,hPygo2
R5714	T20163	T20162	punct	-,mPygo1
R5715	T20165	T20164	auxpass	were,expressed
R5716	T20166	T20164	prep	in,expressed
R5717	T20167	T20166	pobj	bacteria,in
R5718	T20168	T20164	punct	", ",expressed
R5719	T20169	T20164	conj	purified,expressed
R572	T2827	T2826	pobj	Drosophila,in
R5720	T20170	T20169	punct	", ",purified
R5721	T20171	T20169	cc	and,purified
R5722	T20172	T20169	conj	injected,purified
R5723	T20173	T20172	prep	into,injected
R5724	T20174	T20173	pobj	rabbits,into
R5725	T20175	T20172	prep	for,injected
R5726	T20176	T20177	compound	antibody,production
R5727	T20177	T20175	pobj	production,for
R5728	T20178	T20172	agent	by,injected
R5729	T20179	T20178	punct	(,by
R573	T2828	T2825	aux	have,identified
R5730	T20180	T20181	compound	Proteintech,Inc.
R5731	T20181	T20178	pobj	Inc.,by
R5732	T20182	T20181	compound	Group,Inc.
R5733	T20183	T20181	punct	", ",Inc.
R5734	T20184	T20181	npadvmod	Chicago,Inc.
R5735	T20185	T20181	punct	", ",Inc.
R5736	T20186	T20181	npadvmod	IL,Inc.
R5737	T20187	T20181	punct	", ",Inc.
R5738	T20188	T20181	npadvmod	USA,Inc.
R5739	T20189	T20164	punct	),expressed
R574	T2829	T2830	det	the,gene
R5740	T20190	T20164	punct	.,expressed
R5741	T20192	T20193	det	The,antisera
R5742	T20193	T20195	nsubjpass	antisera,allowed
R5743	T20194	T20193	compound	rabbit,antisera
R5744	T20196	T20193	prep	of,antisera
R5745	T20197	T20196	pobj	anti-mPygo1,of
R5746	T20198	T20197	cc	and,anti-mPygo1
R5747	T20199	T20197	conj	anti-hPygo2,anti-mPygo1
R5748	T20200	T20195	auxpass	were,allowed
R5749	T20201	T20195	advmod	initially,allowed
R575	T2830	T2825	dobj	gene,identified
R5750	T20202	T20203	aux	to,bind
R5751	T20203	T20195	xcomp	bind,allowed
R5752	T20204	T20203	prep	to,bind
R5753	T20205	T20206	det	the,matrix
R5754	T20206	T20204	pobj	matrix,to
R5755	T20207	T20206	compound	GST,matrix
R5756	T20208	T20206	compound	affinity,matrix
R5757	T20209	T20210	aux	to,remove
R5758	T20210	T20203	advcl	remove,bind
R5759	T20211	T20212	det	any,antibodies
R576	T2831	T2830	nmod	Pygopus,gene
R5760	T20212	T20210	dobj	antibodies,remove
R5761	T20213	T20212	prep	against,antibodies
R5762	T20214	T20213	pobj	GST,against
R5763	T20215	T20195	punct	.,allowed
R5764	T20217	T20218	det	The,antisera
R5765	T20218	T20222	nsubjpass	antisera,separated
R5766	T20219	T20218	amod	anti-hPygo2,antisera
R5767	T20220	T20219	cc	and,anti-hPygo2
R5768	T20221	T20219	conj	anti-mPygo1,anti-hPygo2
R5769	T20223	T20222	auxpass	were,separated
R577	T2832	T2831	punct	(,Pygopus
R5770	T20224	T20222	advmod	then,separated
R5771	T20225	T20222	prep	from,separated
R5772	T20226	T20227	det	the,matrix
R5773	T20227	T20225	pobj	matrix,from
R5774	T20228	T20227	compound	GST,matrix
R5775	T20229	T20227	compound	affinity,matrix
R5776	T20230	T20222	cc	and,separated
R5777	T20231	T20222	conj	allowed,separated
R5778	T20232	T20233	aux	to,bind
R5779	T20233	T20231	xcomp	bind,allowed
R578	T2833	T2831	appos	Pygo,Pygopus
R5780	T20234	T20233	prep	to,bind
R5781	T20235	T20236	det	the,columns
R5782	T20236	T20234	pobj	columns,to
R5783	T20237	T20238	nmod	GST,hPygo2
R5784	T20238	T20236	nmod	hPygo2,columns
R5785	T20239	T20238	punct	-,hPygo2
R5786	T20240	T20238	cc	or,hPygo2
R5787	T20241	T20242	compound	GST,mPygo1
R5788	T20242	T20238	conj	mPygo1,hPygo2
R5789	T20243	T20242	punct	-,mPygo1
R579	T2834	T2830	punct	),gene
R5790	T20244	T20236	compound	affinity,columns
R5791	T20245	T20233	punct	", ",bind
R5792	T20246	T20233	advmod	respectively,bind
R5793	T20247	T20222	punct	.,separated
R5794	T20249	T20250	det	The,antibodes
R5795	T20250	T20252	nsubjpass	antibodes,eluted
R5796	T20251	T20250	amod	bound,antibodes
R5797	T20253	T20252	auxpass	were,eluted
R5798	T20254	T20252	prep	with,eluted
R5799	T20255	T20256	compound	elution,buffer
R58	T1004	T1005	det	the,orthologs
R580	T2835	T2825	prep	as,identified
R5800	T20256	T20254	pobj	buffer,with
R5801	T20257	T20252	punct	.,eluted
R5802	T20259	T20260	aux	To,ensure
R5803	T20260	T20262	advcl	ensure,incubated
R5804	T20261	T20260	advmod	further,ensure
R5805	T20263	T20264	compound	antibody,specficity
R5806	T20264	T20260	dobj	specficity,ensure
R5807	T20265	T20262	punct	", ",incubated
R5808	T20266	T20267	det	the,antibodies
R5809	T20267	T20262	nsubjpass	antibodies,incubated
R581	T2836	T2837	det	a,component
R5810	T20268	T20267	amod	purified,antibodies
R5811	T20269	T20262	auxpass	were,incubated
R5812	T20270	T20262	advmod	extensively,incubated
R5813	T20271	T20262	prep	with,incubated
R5814	T20272	T20273	npadvmod	Pygo1,homozygous
R5815	T20273	T20278	amod	homozygous,embryo
R5816	T20274	T20272	punct	/,Pygo1
R5817	T20275	T20272	nummod	2,Pygo1
R5818	T20276	T20273	amod	double,homozygous
R5819	T20277	T20273	punct	-,homozygous
R582	T2837	T2835	pobj	component,as
R5820	T20278	T20280	compound	embryo,extract
R5821	T20279	T20278	compound	mutant,embryo
R5822	T20280	T20271	pobj	extract,with
R5823	T20281	T20262	prep	before,incubated
R5824	T20282	T20281	pobj	use,before
R5825	T20283	T20262	punct	.,incubated
R5826	T20633	T20634	compound	BAT,gal
R5827	T20634	T20636	compound	gal,transgene
R5828	T20635	T20634	punct	-,gal
R5829	T20636	T20637	compound	transgene,reporter
R583	T2838	T2837	amod	critical,component
R5830	T20637	T20638	compound	reporter,assay
R5831	T20639	T20638	prep	of,assay
R5832	T20640	T20641	amod	canonical,signaling
R5833	T20641	T20639	pobj	signaling,of
R5834	T20642	T20641	compound	Wnt,signaling
R5835	T20644	T20645	compound	X,gal
R5836	T20645	T20647	compound	gal,staining
R5837	T20646	T20645	punct	-,gal
R5838	T20647	T20648	nsubjpass	staining,performed
R5839	T20649	T20647	prep	of,staining
R584	T2839	T2837	prep	of,component
R5840	T20650	T20651	preconj	both,embryos
R5841	T20651	T20649	pobj	embryos,of
R5842	T20652	T20651	cc	and,embryos
R5843	T20653	T20654	amod	developing,kidneys
R5844	T20654	T20651	conj	kidneys,embryos
R5845	T20655	T20648	auxpass	was,performed
R5846	T20656	T20657	mark	as,described
R5847	T20657	T20648	advcl	described,performed
R5848	T20658	T20657	advmod	previously,described
R5849	T20659	T20660	punct	[,21
R585	T2840	T2841	amod	canonical,signaling
R5850	T20660	T20657	parataxis	21,described
R5851	T20661	T20660	punct	],21
R5852	T20662	T20648	punct	.,performed
R5853	T20664	T20665	nsubjpass	Care,taken
R5854	T20666	T20665	auxpass	was,taken
R5855	T20667	T20668	aux	to,reduce
R5856	T20668	T20665	advcl	reduce,taken
R5857	T20669	T20670	amod	endogenous,activity
R5858	T20670	T20668	dobj	activity,reduce
R5859	T20671	T20672	compound	β,galactosidase
R586	T2841	T2839	pobj	signaling,of
R5860	T20672	T20670	compound	galactosidase,activity
R5861	T20673	T20672	punct	-,galactosidase
R5862	T20674	T20668	prep	within,reduce
R5863	T20675	T20676	det	the,kidney
R5864	T20676	T20674	pobj	kidney,within
R5865	T20677	T20676	amod	developing,kidney
R5866	T20678	T20668	prep	by,reduce
R5867	T20679	T20678	pcomp	increasing,by
R5868	T20680	T20679	dobj	pH,increasing
R5869	T20681	T20680	prep	of,pH
R587	T2842	T2841	compound	Wnt,signaling
R5870	T20682	T20683	det	the,solution
R5871	T20683	T20681	pobj	solution,of
R5872	T20684	T20685	compound	X,gal
R5873	T20685	T20687	compound	gal,staining
R5874	T20686	T20685	punct	-,gal
R5875	T20687	T20683	compound	staining,solution
R5876	T20688	T20679	prep	to,increasing
R5877	T20689	T20688	pobj	8.0,to
R5878	T20690	T20665	punct	.,taken
R5879	T20692	T20693	nsubjpass	Changes,quantitated
R588	T2843	T2844	punct	[,8
R5880	T20694	T20692	prep	in,Changes
R5881	T20695	T20696	compound	transgene,Gal
R5882	T20696	T20699	compound	Gal,expression
R5883	T20697	T20696	compound	β,Gal
R5884	T20698	T20696	punct	-,Gal
R5885	T20699	T20694	pobj	expression,in
R5886	T20700	T20693	auxpass	were,quantitated
R5887	T20701	T20693	advcl	using,quantitated
R5888	T20702	T20703	det	a,kit
R5889	T20703	T20701	dobj	kit,using
R589	T2844	T2825	parataxis	8,identified
R5890	T20704	T20705	nmod	β,Gal
R5891	T20705	T20703	nmod	Gal,kit
R5892	T20706	T20705	punct	-,Gal
R5893	T20707	T20703	nmod	ELISA,kit
R5894	T20708	T20707	punct	(,ELISA
R5895	T20709	T20710	npadvmod	Enzyme,linked
R5896	T20710	T20712	amod	linked,immunoassay
R5897	T20711	T20710	punct	-,linked
R5898	T20712	T20707	appos	immunoassay,ELISA
R5899	T20713	T20703	punct	),kit
R59	T1005	T1003	pobj	orthologs,in
R590	T2845	T2846	punct	-,11
R5900	T20714	T20715	punct	(,Roche
R5901	T20715	T20703	parataxis	Roche,kit
R5902	T20716	T20715	punct	", ",Roche
R5903	T20717	T20715	npadvmod	Indianapolis,Roche
R5904	T20718	T20715	punct	", ",Roche
R5905	T20719	T20715	npadvmod	IN,Roche
R5906	T20720	T20715	punct	),Roche
R5907	T20721	T20693	punct	", ",quantitated
R5908	T20722	T20693	advcl	normalizing,quantitated
R5909	T20723	T20722	prep	according,normalizing
R591	T2846	T2844	prep	11,8
R5910	T20724	T20723	prep	to,according
R5911	T20725	T20726	amod	total,protein
R5912	T20726	T20724	pobj	protein,to
R5913	T20727	T20693	punct	.,quantitated
R5914	T20729	T20730	det	Each,genotype
R5915	T20730	T20731	nsubjpass	genotype,represented
R5916	T20732	T20731	auxpass	was,represented
R5917	T20733	T20731	agent	by,represented
R5918	T20734	T20735	det	a,size
R5919	T20735	T20733	pobj	size,by
R592	T2847	T2844	punct	],8
R5920	T20736	T20735	compound	sample,size
R5921	T20737	T20735	prep	of,size
R5922	T20738	T20737	pobj	4,of
R5923	T20739	T20731	prep	except,represented
R5924	T20740	T20741	det	the,groups
R5925	T20741	T20739	pobj	groups,except
R5926	T20742	T20741	amod	non-transgenic,groups
R5927	T20743	T20744	punct	(,5
R5928	T20744	T20742	parataxis	5,non-transgenic
R5929	T20745	T20744	nsubj	n,5
R593	T2848	T2825	punct	.,identified
R5930	T20746	T20744	punct	=,5
R5931	T20747	T20744	punct	),5
R5932	T20748	T20742	punct	", ",non-transgenic
R5933	T20749	T20742	conj	Pygo1,non-transgenic
R5934	T20750	T20749	punct	+,Pygo1
R5935	T20751	T20749	punct	/,Pygo1
R5936	T20752	T20749	punct	-,Pygo1
R5937	T20753	T20749	punct	;,Pygo1
R5938	T20754	T20749	appos	Pygo2,Pygo1
R5939	T20755	T20754	punct	+,Pygo2
R594	T2850	T2851	nsubj	Pygo,interact
R5940	T20756	T20754	punct	/,Pygo2
R5941	T20757	T20754	punct	+,Pygo2
R5942	T20758	T20759	punct	(,6
R5943	T20759	T20749	parataxis	6,Pygo1
R5944	T20760	T20759	nsubj	n,6
R5945	T20761	T20759	punct	=,6
R5946	T20762	T20759	punct	),6
R5947	T20763	T20749	punct	", ",Pygo1
R5948	T20764	T20749	conj	Pygo1,Pygo1
R5949	T20765	T20764	punct	-,Pygo1
R595	T2852	T2850	cc	and,Pygo
R5950	T20766	T20764	punct	/,Pygo1
R5951	T20767	T20764	punct	-,Pygo1
R5952	T20768	T20764	punct	;,Pygo1
R5953	T20769	T20764	appos	Pygo2,Pygo1
R5954	T20770	T20769	punct	+,Pygo2
R5955	T20771	T20769	punct	/,Pygo2
R5956	T20772	T20769	punct	-,Pygo2
R5957	T20773	T20774	punct	(,8
R5958	T20774	T20764	parataxis	8,Pygo1
R5959	T20775	T20774	nsubj	n,8
R596	T2853	T2850	conj	Lgs,Pygo
R5960	T20776	T20774	punct	=,8
R5961	T20777	T20774	punct	),8
R5962	T20778	T20764	punct	", ",Pygo1
R5963	T20779	T20764	cc	and,Pygo1
R5964	T20780	T20764	conj	Pygo1,Pygo1
R5965	T20781	T20780	punct	-,Pygo1
R5966	T20782	T20780	punct	/,Pygo1
R5967	T20783	T20780	punct	-,Pygo1
R5968	T20784	T20780	punct	;,Pygo1
R5969	T20785	T20780	appos	Pygo2,Pygo1
R597	T2854	T2851	prep	with,interact
R5970	T20786	T20785	punct	-,Pygo2
R5971	T20787	T20785	punct	/,Pygo2
R5972	T20788	T20785	punct	-,Pygo2
R5973	T20789	T20790	punct	(,6
R5974	T20790	T20780	parataxis	6,Pygo1
R5975	T20791	T20790	nsubj	n,6
R5976	T20792	T20790	punct	=,6
R5977	T20793	T20790	punct	),6
R5978	T20794	T20731	punct	.,represented
R5979	T21192	T21193	compound	Microarray,analysis
R598	T2855	T2856	compound	β,catenin
R5980	T21195	T21196	amod	Total,RNA
R5981	T21196	T21197	nsubjpass	RNA,isolated
R5982	T21198	T21197	auxpass	was,isolated
R5983	T21199	T21197	prep	from,isolated
R5984	T21200	T21201	compound	E18.5,kidneys
R5985	T21201	T21199	pobj	kidneys,from
R5986	T21202	T21201	acl	dissected,kidneys
R5987	T21203	T21202	prep	from,dissected
R5988	T21204	T21205	amod	normal,embryos
R5989	T21205	T21203	pobj	embryos,from
R599	T2856	T2854	pobj	catenin,with
R5990	T21206	T21204	cc	and,normal
R5991	T21207	T21208	compound	Pygo1,Pygo2
R5992	T21208	T21210	npadvmod	Pygo2,null
R5993	T21209	T21208	punct	/,Pygo2
R5994	T21210	T21204	conj	null,normal
R5995	T21211	T21210	npadvmod	compound,null
R5996	T21212	T21197	advcl	using,isolated
R5997	T21213	T21214	det	a,kit
R5998	T21214	T21212	dobj	kit,using
R5999	T21215	T21214	amod	commercial,kit
R60	T1006	T1005	nummod	two,orthologs
R600	T2857	T2856	punct	-,catenin
R6000	T21216	T21214	punct	(,kit
R6001	T21217	T21218	compound	Stratagene,Kit
R6002	T21218	T21214	appos	Kit,kit
R6003	T21219	T21218	compound	Absolutely,Kit
R6004	T21220	T21218	compound	RNA,Kit
R6005	T21221	T21218	compound	Microprep,Kit
R6006	T21222	T21223	punct	;,USA
R6007	T21223	T21214	parataxis	USA,kit
R6008	T21224	T21225	compound	La,Jolla
R6009	T21225	T21223	npadvmod	Jolla,USA
R601	T2858	T2851	prep	during,interact
R6010	T21226	T21223	punct	", ",USA
R6011	T21227	T21223	npadvmod	CA,USA
R6012	T21228	T21223	punct	", ",USA
R6013	T21229	T21223	punct	),USA
R6014	T21230	T21197	punct	.,isolated
R6015	T21232	T21233	det	An,aliquot
R6016	T21233	T21234	nsubjpass	aliquot,processed
R6017	T21235	T21236	punct	(,ng
R6018	T21236	T21233	parataxis	ng,aliquot
R6019	T21237	T21236	nummod	300,ng
R602	T2859	T2860	det	the,formation
R6020	T21238	T21236	punct	),ng
R6021	T21239	T21233	prep	of,aliquot
R6022	T21240	T21241	amod	total,RNA
R6023	T21241	T21239	pobj	RNA,of
R6024	T21242	T21234	auxpass	was,processed
R6025	T21243	T21234	cc	and,processed
R6026	T21244	T21234	conj	labeled,processed
R6027	T21245	T21244	advcl	using,labeled
R6028	T21246	T21247	det	a,kit
R6029	T21247	T21245	dobj	kit,using
R603	T2860	T2858	pobj	formation,during
R6030	T21248	T21247	amod	commercial,kit
R6031	T21249	T21247	punct	(,kit
R6032	T21250	T21251	nmod	TargetAmp,Ki
R6033	T21251	T21247	appos	Ki,kit
R6034	T21252	T21253	nummod	1,Round
R6035	T21253	T21251	nmod	Round,Ki
R6036	T21254	T21253	punct	-,Round
R6037	T21255	T21256	compound	Aminoallyl,aRNA
R6038	T21256	T21251	compound	aRNA,Ki
R6039	T21257	T21256	punct	-,aRNA
R604	T2861	T2860	prep	of,formation
R6040	T21258	T21259	punct	;,Epicentre
R6041	T21259	T21247	parataxis	Epicentre,kit
R6042	T21260	T21259	punct	", ",Epicentre
R6043	T21261	T21259	npadvmod	Madison,Epicentre
R6044	T21262	T21259	punct	", ",Epicentre
R6045	T21263	T21259	npadvmod	WI,Epicentre
R6046	T21264	T21259	punct	", ",Epicentre
R6047	T21265	T21259	npadvmod	USA,Epicentre
R6048	T21266	T21259	punct	),Epicentre
R6049	T21267	T21234	punct	.,processed
R605	T2862	T2863	det	the,complex
R6050	T21269	T21270	amod	Labeled,RNA
R6051	T21270	T21271	nsubjpass	RNA,hybridized
R6052	T21272	T21271	auxpass	was,hybridized
R6053	T21273	T21271	prep	to,hybridized
R6054	T21274	T21273	pobj	microarrays,to
R6055	T21275	T21274	punct	(,microarrays
R6056	T21276	T21277	nmod	Sentrix,Beadchip
R6057	T21277	T21274	appos	Beadchip,microarrays
R6058	T21278	T21277	nmod	Mouse,Beadchip
R6059	T21279	T21278	punct	-,Mouse
R606	T2863	T2861	pobj	complex,of
R6060	T21280	T21278	nummod	6,Mouse
R6061	T21281	T21277	compound	expression,Beadchip
R6062	T21282	T21283	punct	;,Illumina
R6063	T21283	T21274	parataxis	Illumina,microarrays
R6064	T21284	T21283	punct	", ",Illumina
R6065	T21285	T21286	compound	San,Diego
R6066	T21286	T21283	npadvmod	Diego,Illumina
R6067	T21287	T21283	punct	", ",Illumina
R6068	T21288	T21283	npadvmod	CA,Illumina
R6069	T21289	T21283	punct	),Illumina
R607	T2864	T2863	amod	canonical,complex
R6070	T21290	T21274	acl	providing,microarrays
R6071	T21291	T21290	dobj	coverage,providing
R6072	T21292	T21291	prep	of,coverage
R6073	T21293	T21294	quantmod	over,47000
R6074	T21294	T21295	nummod	47000,genes
R6075	T21295	T21292	pobj	genes,of
R6076	T21296	T21271	cc	and,hybridized
R6077	T21297	T21271	conj	expressed,hybridized
R6078	T21298	T21299	compound	sequence,tags
R6079	T21299	T21297	dobj	tags,expressed
R608	T2865	T2863	amod	transcriptional,complex
R6080	T21300	T21301	mark	as,described
R6081	T21301	T21297	advcl	described,expressed
R6082	T21302	T21301	advmod	previously,described
R6083	T21303	T21304	punct	[,36
R6084	T21304	T21301	parataxis	36,described
R6085	T21305	T21304	punct	],36
R6086	T21306	T21271	punct	.,hybridized
R6087	T21308	T21309	compound	Microarray,analysis
R6088	T21309	T21310	nsubjpass	analysis,performed
R6089	T21311	T21309	prep	of,analysis
R609	T2866	T2851	cc	and,interact
R6090	T21312	T21313	compound	Pygo1,Pygo2
R6091	T21313	T21315	npadvmod	Pygo2,null
R6092	T21314	T21313	punct	/,Pygo2
R6093	T21315	T21311	pobj	null,of
R6094	T21316	T21315	cc	and,null
R6095	T21317	T21318	amod	normal,type
R6096	T21318	T21321	compound	type,kidneys
R6097	T21319	T21318	amod	wild,type
R6098	T21320	T21318	punct	-,type
R6099	T21321	T21315	conj	kidneys,null
R61	T1007	T1005	amod	mammalian,orthologs
R610	T2867	T2868	auxpass	are,required
R6100	T21322	T21310	auxpass	was,performed
R6101	T21323	T21310	prep	in,performed
R6102	T21324	T21325	amod	biological,triplicate
R6103	T21325	T21323	pobj	triplicate,in
R6104	T21326	T21310	punct	.,performed
R6105	T21328	T21329	amod	Raw,intensities
R6106	T21329	T21331	nsubjpass	intensities,obtained
R6107	T21330	T21329	compound	signal,intensities
R6108	T21332	T21329	prep	of,intensities
R6109	T21333	T21334	det	each,probe
R611	T2868	T2851	conj	required,interact
R6110	T21334	T21332	pobj	probe,of
R6111	T21335	T21331	auxpass	were,obtained
R6112	T21336	T21331	prep	from,obtained
R6113	T21337	T21338	compound	data,software
R6114	T21338	T21336	pobj	software,from
R6115	T21339	T21338	compound	analysis,software
R6116	T21340	T21338	punct	(,software
R6117	T21341	T21338	appos	Beadstudio,software
R6118	T21342	T21343	punct	;,Illumina
R6119	T21343	T21338	parataxis	Illumina,software
R612	T2869	T2868	prep	for,required
R6120	T21344	T21343	punct	),Illumina
R6121	T21345	T21331	cc	and,obtained
R6122	T21346	T21331	conj	imported,obtained
R6123	T21347	T21346	prep	into,imported
R6124	T21348	T21349	compound	GeneSpring,GX
R6125	T21349	T21347	pobj	GX,into
R6126	T21350	T21349	nummod	7.3,GX
R6127	T21351	T21352	punct	(,Technologies
R6128	T21352	T21349	parataxis	Technologies,GX
R6129	T21353	T21352	compound	Agilent,Technologies
R613	T2870	T2869	pobj	accumulation,for
R6130	T21354	T21352	punct	", ",Technologies
R6131	T21355	T21356	compound	Palo,Alto
R6132	T21356	T21352	npadvmod	Alto,Technologies
R6133	T21357	T21352	punct	", ",Technologies
R6134	T21358	T21352	npadvmod	CA,Technologies
R6135	T21359	T21352	punct	", ",Technologies
R6136	T21360	T21352	npadvmod	USA,Technologies
R6137	T21361	T21352	punct	),Technologies
R6138	T21362	T21331	punct	.,obtained
R6139	T21364	T21365	nsubjpass	Genes,selected
R614	T2871	T2870	prep	of,accumulation
R6140	T21366	T21365	auxpass	were,selected
R6141	T21367	T21365	prep	on,selected
R6142	T21368	T21369	det	the,basis
R6143	T21369	T21367	pobj	basis,on
R6144	T21370	T21369	prep	of,basis
R6145	T21371	T21372	amod	greater,two-fold
R6146	T21372	T21374	nummod	two-fold,change
R6147	T21373	T21372	quantmod	than,two-fold
R6148	T21374	T21370	pobj	change,of
R6149	T21375	T21374	amod	average,change
R615	T2872	T2873	compound	β,catenin
R6150	T21376	T21374	compound	fold,change
R6151	T21377	T21374	cc	and,change
R6152	T21378	T21379	amod	statistical,significance
R6153	T21379	T21374	conj	significance,change
R6154	T21380	T21381	punct	(,0.05
R6155	T21381	T21379	parataxis	0.05,significance
R6156	T21382	T21383	compound	p,value
R6157	T21383	T21381	nsubj	value,0.05
R6158	T21384	T21383	punct	-,value
R6159	T21385	T21381	punct	<,0.05
R616	T2873	T2871	pobj	catenin,of
R6160	T21386	T21381	punct	),0.05
R6161	T21387	T21365	punct	.,selected
R6162	T21389	T21390	advmod	Previously,described
R6163	T21390	T21391	amod	described,genes
R6164	T21391	T21394	nsubjpass	genes,obtained
R6165	T21392	T21391	compound	Wnt,genes
R6166	T21393	T21391	compound	target,genes
R6167	T21395	T21394	auxpass	were,obtained
R6168	T21396	T21394	prep	from,obtained
R6169	T21397	T21394	punct	.,obtained
R617	T2874	T2873	punct	-,catenin
R6170	T21853	T21854	amod	Quantitative,validation
R6171	T21855	T21854	compound	PCR,validation
R6172	T21856	T21854	prep	of,validation
R6173	T21857	T21858	compound	microarray,results
R6174	T21858	T21856	pobj	results,of
R6175	T21860	T21861	amod	Total,RNA
R6176	T21861	T21862	nsubjpass	RNA,purified
R6177	T21863	T21861	prep	from,RNA
R6178	T21864	T21865	nmod	E18.5,kidneys
R6179	T21865	T21863	pobj	kidneys,from
R618	T2875	T2870	prep	in,accumulation
R6180	T21866	T21867	compound	Pygo1,Pygo2
R6181	T21867	T21869	npadvmod	Pygo2,null
R6182	T21868	T21867	punct	/,Pygo2
R6183	T21869	T21865	amod	null,kidneys
R6184	T21870	T21869	cc	and,null
R6185	T21871	T21869	conj	control,null
R6186	T21872	T21861	punct	(,RNA
R6187	T21873	T21874	preconj	both,represented
R6188	T21874	T21861	acl	represented,RNA
R6189	T21875	T21874	prep	in,represented
R619	T2876	T2877	det	the,nucleus
R6190	T21876	T21875	pobj	duplicate,in
R6191	T21877	T21874	cc	and,represented
R6192	T21878	T21874	conj	distinct,represented
R6193	T21879	T21878	prep	from,distinct
R6194	T21880	T21881	det	the,kidneys
R6195	T21881	T21879	pobj	kidneys,from
R6196	T21882	T21881	acl	used,kidneys
R6197	T21883	T21882	prep	for,used
R6198	T21884	T21885	compound	microrray,analysis
R6199	T21885	T21883	pobj	analysis,for
R62	T1008	T1005	punct	", ",orthologs
R620	T2877	T2875	pobj	nucleus,in
R6200	T21886	T21862	punct	),purified
R6201	T21887	T21862	auxpass	was,purified
R6202	T21888	T21862	advcl	using,purified
R6203	T21889	T21890	det	a,kit
R6204	T21890	T21888	dobj	kit,using
R6205	T21891	T21890	amod	commercial,kit
R6206	T21892	T21890	punct	(,kit
R6207	T21893	T21894	compound	Absolutely,Kit
R6208	T21894	T21890	appos	Kit,kit
R6209	T21895	T21894	compound	RNA,Kit
R621	T2878	T2879	punct	[,12
R6210	T21896	T21894	compound	Microprep,Kit
R6211	T21897	T21898	punct	;,Stratagene
R6212	T21898	T21890	parataxis	Stratagene,kit
R6213	T21899	T21898	punct	", ",Stratagene
R6214	T21900	T21901	compound	La,Jolla
R6215	T21901	T21898	npadvmod	Jolla,Stratagene
R6216	T21902	T21898	punct	", ",Stratagene
R6217	T21903	T21904	compound	CA,USA
R6218	T21904	T21898	npadvmod	USA,Stratagene
R6219	T21905	T21898	punct	),Stratagene
R622	T2879	T2868	parataxis	12,required
R6220	T21906	T21890	prep	including,kit
R6221	T21907	T21908	compound	DNAse1,treatment
R6222	T21908	T21906	pobj	treatment,including
R6223	T21909	T21862	punct	.,purified
R6224	T21911	T21912	nsubjpass	cDNA,generated
R6225	T21913	T21912	auxpass	was,generated
R6226	T21914	T21912	advcl	using,generated
R6227	T21915	T21916	amod	random,hexamers
R6228	T21916	T21914	dobj	hexamers,using
R6229	T21917	T21914	prep	according,using
R623	T2880	T2879	punct	],12
R6230	T21918	T21917	prep	to,according
R6231	T21919	T21920	amod	conventional,protocols
R6232	T21920	T21918	pobj	protocols,to
R6233	T21921	T21922	punct	(,Invitrogen
R6234	T21922	T21920	parataxis	Invitrogen,protocols
R6235	T21923	T21922	punct	", ",Invitrogen
R6236	T21924	T21922	npadvmod	Carlsbad,Invitrogen
R6237	T21925	T21922	punct	", ",Invitrogen
R6238	T21926	T21922	npadvmod	CA,Invitrogen
R6239	T21927	T21922	punct	", ",Invitrogen
R624	T2881	T2851	punct	.,interact
R6240	T21928	T21922	npadvmod	USA,Invitrogen
R6241	T21929	T21922	punct	),Invitrogen
R6242	T21930	T21912	punct	.,generated
R6243	T21932	T21933	det	The,primers
R6244	T21933	T21935	nsubjpass	primers,generated
R6245	T21934	T21933	amod	following,primers
R6246	T21936	T21935	auxpass	were,generated
R6247	T21937	T21938	aux	to,include
R6248	T21938	T21935	advcl	include,generated
R6249	T21939	T21940	det	the,sequence
R625	T2883	T2884	nsubj	Lgs,binds
R6250	T21940	T21938	dobj	sequence,include
R6251	T21941	T21940	acl	obtained,sequence
R6252	T21942	T21941	prep	from,obtained
R6253	T21943	T21944	det	the,probe
R6254	T21944	T21942	pobj	probe,from
R6255	T21945	T21944	compound	Illumina,probe
R6256	T21946	T21935	punct	: ,generated
R6257	T21947	T21935	dobj	Actb,generated
R6258	T21948	T21949	punct	(,ACATCTGCTGGAAGGTGGAC
R6259	T21949	T21947	parataxis	ACATCTGCTGGAAGGTGGAC,Actb
R626	T2885	T2886	det	the,repeats
R6260	T21950	T21949	nmod	TTGCTGACAGGATGCAGAAG,ACATCTGCTGGAAGGTGGAC
R6261	T21951	T21949	punct	", ",ACATCTGCTGGAAGGTGGAC
R6262	T21952	T21949	punct	),ACATCTGCTGGAAGGTGGAC
R6263	T21953	T21947	punct	;,Actb
R6264	T21954	T21947	conj	Aldh1a7,Actb
R6265	T21955	T21956	punct	(,GAGTTACAGAGAGCTTGCACCTT
R6266	T21956	T21954	parataxis	GAGTTACAGAGAGCTTGCACCTT,Aldh1a7
R6267	T21957	T21956	nmod	CCAGAAAGTGGTGTTTTGCT,GAGTTACAGAGAGCTTGCACCTT
R6268	T21958	T21956	punct	", ",GAGTTACAGAGAGCTTGCACCTT
R6269	T21959	T21956	punct	),GAGTTACAGAGAGCTTGCACCTT
R627	T2886	T2884	dobj	repeats,binds
R6270	T21960	T21954	punct	;,Aldh1a7
R6271	T21961	T21954	conj	Col8a1,Aldh1a7
R6272	T21962	T21963	punct	(,TGTGTACATCATGGGCTCGT
R6273	T21963	T21961	parataxis	TGTGTACATCATGGGCTCGT,Col8a1
R6274	T21964	T21963	nmod	GCAGACAGGCATCTTCACCT,TGTGTACATCATGGGCTCGT
R6275	T21965	T21963	punct	", ",TGTGTACATCATGGGCTCGT
R6276	T21966	T21963	punct	),TGTGTACATCATGGGCTCGT
R6277	T21967	T21961	punct	;,Col8a1
R6278	T21968	T21961	conj	Csrp1,Col8a1
R6279	T21969	T21970	punct	(,TGGGCAAGGTAGTGAAGGTT
R628	T2887	T2886	amod	central,repeats
R6280	T21970	T21968	parataxis	TGGGCAAGGTAGTGAAGGTT,Csrp1
R6281	T21971	T21970	nmod	CAGCATAGCCCAGGGTAGAG,TGGGCAAGGTAGTGAAGGTT
R6282	T21972	T21970	punct	", ",TGGGCAAGGTAGTGAAGGTT
R6283	T21973	T21970	punct	),TGGGCAAGGTAGTGAAGGTT
R6284	T21974	T21968	punct	;,Csrp1
R6285	T21975	T21968	conj	Klk5,Csrp1
R6286	T21976	T21977	punct	(,TTGCCTCCATCCATATCTCC
R6287	T21977	T21975	parataxis	TTGCCTCCATCCATATCTCC,Klk5
R6288	T21978	T21977	nmod	GCAGCATTACACCCGTCATA,TTGCCTCCATCCATATCTCC
R6289	T21979	T21977	punct	", ",TTGCCTCCATCCATATCTCC
R629	T2888	T2886	compound	armadillo,repeats
R6290	T21980	T21977	punct	),TTGCCTCCATCCATATCTCC
R6291	T21981	T21975	punct	;,Klk5
R6292	T21982	T21975	conj	Picalm,Klk5
R6293	T21983	T21984	punct	(,GCACCGATCAACAGTGCAG
R6294	T21984	T21982	parataxis	GCACCGATCAACAGTGCAG,Picalm
R6295	T21985	T21984	nmod	GGGAGGGAACAGAAATCCTT,GCACCGATCAACAGTGCAG
R6296	T21986	T21984	punct	", ",GCACCGATCAACAGTGCAG
R6297	T21987	T21984	punct	),GCACCGATCAACAGTGCAG
R6298	T21988	T21982	punct	;,Picalm
R6299	T21989	T21982	conj	Pygo2,Picalm
R63	T1009	T1005	appos	Pygo1,orthologs
R630	T2889	T2886	prep	of,repeats
R6300	T21990	T21991	punct	(,AACTTCCTCCAGCCCATTTT
R6301	T21991	T21989	parataxis	AACTTCCTCCAGCCCATTTT,Pygo2
R6302	T21992	T21991	nmod	TTCTGGGAACTTGTGCACTG,AACTTCCTCCAGCCCATTTT
R6303	T21993	T21991	punct	", ",AACTTCCTCCAGCCCATTTT
R6304	T21994	T21991	punct	),AACTTCCTCCAGCCCATTTT
R6305	T21995	T21989	punct	;,Pygo2
R6306	T21996	T21989	conj	Ren2,Pygo2
R6307	T21997	T21998	punct	(,TGTGCACAGCTTGTCTCTCC
R6308	T21998	T21996	parataxis	TGTGCACAGCTTGTCTCTCC,Ren2
R6309	T21999	T21998	nmod	TTGTGAGCTGTAGCCAGGTG,TGTGCACAGCTTGTCTCTCC
R631	T2890	T2891	compound	β,catenin
R6310	T22000	T21998	punct	", ",TGTGCACAGCTTGTCTCTCC
R6311	T22001	T21998	punct	),TGTGCACAGCTTGTCTCTCC
R6312	T22002	T21996	cc	and,Ren2
R6313	T22003	T21996	conj	Tia1,Ren2
R6314	T22004	T22005	punct	(,AGCCCCAGAGGACAATTTTT
R6315	T22005	T22003	parataxis	AGCCCCAGAGGACAATTTTT,Tia1
R6316	T22006	T22005	nmod	TGATTGAAGGGCTACTAGAGTGGT,AGCCCCAGAGGACAATTTTT
R6317	T22007	T22005	punct	", ",AGCCCCAGAGGACAATTTTT
R6318	T22008	T22005	punct	),AGCCCCAGAGGACAATTTTT
R6319	T22009	T21935	advcl	using,generated
R632	T2891	T2889	pobj	catenin,of
R6320	T22010	T22011	compound	Primer3,software
R6321	T22011	T22009	dobj	software,using
R6322	T22012	T22013	punct	[,37
R6323	T22013	T22011	parataxis	37,software
R6324	T22014	T22013	punct	],37
R6325	T22015	T21935	punct	.,generated
R6326	T22017	T22018	amod	Relative,PCR
R6327	T22018	T22020	nsubjpass	PCR,performed
R6328	T22019	T22018	amod	quantitative,PCR
R6329	T22021	T22020	auxpass	was,performed
R633	T2892	T2891	punct	-,catenin
R6330	T22022	T22020	prep	according,performed
R6331	T22023	T22022	prep	to,according
R6332	T22024	T22025	det	the,protocol
R6333	T22025	T22023	pobj	protocol,to
R6334	T22026	T22025	amod	conventional,protocol
R6335	T22027	T22028	compound	SYBR,Green
R6336	T22028	T22025	compound	Green,protocol
R6337	T22029	T22030	punct	(,Stratagene
R6338	T22030	T22025	parataxis	Stratagene,protocol
R6339	T22031	T22030	punct	),Stratagene
R634	T2893	T2884	punct	", ",binds
R6340	T22032	T22020	advcl	using,performed
R6341	T22033	T22034	det	a,system
R6342	T22034	T22032	dobj	system,using
R6343	T22035	T22034	amod	quantitative,system
R6344	T22036	T22034	compound	PCR,system
R6345	T22037	T22038	punct	(,Stratagene
R6346	T22038	T22032	parataxis	Stratagene,using
R6347	T22039	T22038	dep	Mx3000p,Stratagene
R6348	T22040	T22038	punct	;,Stratagene
R6349	T22041	T22038	punct	),Stratagene
R635	T2894	T2895	mark	while,interacts
R6350	T22042	T22020	punct	.,performed
R6351	T22044	T22045	nmod	Dissociation,curve
R6352	T22045	T22046	nmod	curve,analysis
R6353	T22046	T22051	nsubjpass	analysis,used
R6354	T22047	T22045	cc	and,curve
R6355	T22048	T22049	compound	agarose,gel
R6356	T22049	T22045	conj	gel,curve
R6357	T22050	T22049	punct	-,gel
R6358	T22052	T22046	prep	of,analysis
R6359	T22053	T22054	det	each,primer
R636	T2895	T2884	advcl	interacts,binds
R6360	T22054	T22052	pobj	primer,of
R6361	T22055	T22054	amod	set,primer
R6362	T22056	T22051	auxpass	were,used
R6363	T22057	T22058	aux	to,insure
R6364	T22058	T22051	advcl	insure,used
R6365	T22059	T22058	dobj	specificity,insure
R6366	T22060	T22059	prep	of,specificity
R6367	T22061	T22062	det	the,amplicon
R6368	T22062	T22060	pobj	amplicon,of
R6369	T22063	T22051	punct	.,used
R637	T2896	T2895	nsubj	Pygo,interacts
R6370	T22065	T22066	det	All,data
R6371	T22066	T22067	nsubjpass	data,normalized
R6372	T22068	T22067	auxpass	were,normalized
R6373	T22069	T22067	prep	to,normalized
R6374	T22070	T22071	det	an,control
R6375	T22071	T22069	pobj	control,to
R6376	T22072	T22071	amod	internal,control
R6377	T22073	T22071	compound	housekeeping,control
R6378	T22074	T22071	punct	(,control
R6379	T22075	T22071	appos	Actb,control
R638	T2897	T2895	prep	with,interacts
R6380	T22076	T22067	punct	),normalized
R6381	T22077	T22067	cc	and,normalized
R6382	T22078	T22067	conj	analyzed,normalized
R6383	T22079	T22078	advcl	using,analyzed
R6384	T22080	T22081	det	the,method
R6385	T22081	T22079	dobj	method,using
R6386	T22082	T22083	nummod	2,T
R6387	T22083	T22081	nmod	T,method
R6388	T22084	T22083	punct	(,T
R6389	T22085	T22083	punct	-,T
R639	T2898	T2897	pobj	Lgs,with
R6390	T22086	T22083	nmod	Delta,T
R6391	T22087	T22083	nmod	Delta,T
R6392	T22088	T22083	nmod	C,T
R6393	T22089	T22083	punct	(,T
R6394	T22090	T22083	punct	),T
R6395	T22091	T22083	punct	),T
R6396	T22092	T22093	punct	[,38
R6397	T22093	T22078	parataxis	38,analyzed
R6398	T22094	T22093	punct	],38
R6399	T22095	T22067	punct	.,normalized
R64	T1010	T1009	cc	and,Pygo1
R640	T2899	T2895	punct	", ",interacts
R6400	T22293	T22294	compound	Pygo1,Pygo2
R6401	T22294	T22296	compound	Pygo2,mutant
R6402	T22295	T22294	punct	/,Pygo2
R6403	T22296	T22297	compound	mutant,embryos
R6404	T22298	T22297	punct	.,embryos
R6405	T22300	T22301	punct	(,A
R6406	T22301	T22302	meta	A,appeared
R6407	T22303	T22301	punct	),A
R6408	T22304	T22305	nmod	E18.5,embryos
R6409	T22305	T22302	nsubj	embryos,appeared
R641	T2900	T2895	advcl	mediating,interacts
R6410	T22306	T22305	nmod	Pygo1,embryos
R6411	T22307	T22306	punct	-,Pygo1
R6412	T22308	T22306	punct	/,Pygo1
R6413	T22309	T22306	punct	-,Pygo1
R6414	T22310	T22306	punct	/,Pygo1
R6415	T22311	T22306	appos	Pygo2,Pygo1
R6416	T22312	T22311	punct	+,Pygo2
R6417	T22313	T22311	punct	/,Pygo2
R6418	T22314	T22311	punct	-,Pygo2
R6419	T22315	T22305	punct	", ",embryos
R642	T2901	T2900	dobj	activation,mediating
R6420	T22316	T22305	prep	with,embryos
R6421	T22317	T22318	advmod	only,allele
R6422	T22318	T22316	pobj	allele,with
R6423	T22319	T22318	nummod	one,allele
R6424	T22320	T22321	amod	wild,type
R6425	T22321	T22318	compound	type,allele
R6426	T22322	T22321	punct	-,type
R6427	T22323	T22318	compound	Pygo2,allele
R6428	T22324	T22302	punct	", ",appeared
R6429	T22325	T22302	oprd	normal,appeared
R643	T2902	T2901	prep	of,activation
R6430	T22326	T22302	punct	.,appeared
R6431	T22328	T22329	punct	(,B
R6432	T22329	T22330	meta	B,were
R6433	T22331	T22329	punct	", ",B
R6434	T22332	T22329	dep	C,B
R6435	T22333	T22329	punct	),B
R6436	T22334	T22335	amod	Double,homozygous
R6437	T22335	T22336	amod	homozygous,mutant
R6438	T22336	T22337	nmod	mutant,embryos
R6439	T22337	T22330	nsubj	embryos,were
R644	T2903	T2904	compound	Wnt,targets
R6440	T22338	T22337	nmod	Pygo1,embryos
R6441	T22339	T22338	punct	-,Pygo1
R6442	T22340	T22338	punct	/,Pygo1
R6443	T22341	T22338	punct	-,Pygo1
R6444	T22342	T22338	punct	/,Pygo1
R6445	T22343	T22338	appos	Pygo2,Pygo1
R6446	T22344	T22343	punct	-,Pygo2
R6447	T22345	T22343	punct	/,Pygo2
R6448	T22346	T22343	punct	-,Pygo2
R6449	T22347	T22330	acomp	smaller,were
R645	T2904	T2902	pobj	targets,of
R6450	T22348	T22330	punct	", ",were
R6451	T22349	T22330	prep	with,were
R6452	T22350	T22351	compound	eye,defects
R6453	T22351	T22349	pobj	defects,with
R6454	T22352	T22351	prep	including,defects
R6455	T22353	T22354	amod	absent,lens
R6456	T22354	T22352	pobj	lens,including
R6457	T22355	T22353	cc	or,absent
R6458	T22356	T22353	conj	rudimentary,absent
R6459	T22357	T22354	cc	and,lens
R646	T2905	T2906	punct	[,13
R6460	T22358	T22359	amod	folded,retina
R6461	T22359	T22354	conj	retina,lens
R6462	T22360	T22359	amod	pigmented,retina
R6463	T22361	T22362	punct	(,arrows
R6464	T22362	T22330	parataxis	arrows,were
R6465	T22363	T22362	punct	),arrows
R6466	T22364	T22330	punct	.,were
R6467	T22366	T22367	punct	(,C
R6468	T22367	T22368	meta	C,displayed
R6469	T22369	T22367	punct	),C
R647	T2906	T2895	parataxis	13,interacts
R6470	T22370	T22371	det	A,percentage
R6471	T22371	T22368	nsubj	percentage,displayed
R6472	T22372	T22371	amod	small,percentage
R6473	T22373	T22371	prep	of,percentage
R6474	T22374	T22375	compound	Pygo1,Pygo2
R6475	T22375	T22377	npadvmod	Pygo2,null
R6476	T22376	T22375	punct	/,Pygo2
R6477	T22377	T22378	amod	null,embryos
R6478	T22378	T22373	pobj	embryos,of
R6479	T22379	T22368	advmod	also,displayed
R648	T2907	T2906	nummod	9,13
R6480	T22380	T22368	dobj	exencephaly,displayed
R6481	T22381	T22368	punct	.,displayed
R6484	T22637	T22638	nmod	Pygo1,expression
R6485	T22639	T22637	cc	and,Pygo1
R6486	T22640	T22637	conj	Pygo2,Pygo1
R6487	T22641	T22638	prep	in,expression
R6488	T22642	T22643	det	the,kidney
R6489	T22643	T22641	pobj	kidney,in
R649	T2908	T2906	punct	",",13
R6490	T22644	T22643	nmod	E18.5,kidney
R6491	T22645	T22643	amod	developing,kidney
R6492	T22646	T22638	punct	.,expression
R6493	T22648	T22649	nsubjpass	Immunofluorescence,used
R6494	T22650	T22649	auxpass	was,used
R6495	T22651	T22652	aux	to,determine
R6496	T22652	T22649	advcl	determine,used
R6497	T22653	T22654	compound	expression,patterns
R6498	T22654	T22652	dobj	patterns,determine
R6499	T22655	T22654	prep	of,patterns
R65	T1011	T1009	conj	Pygo2,Pygo1
R650	T2909	T2906	punct	],13
R6500	T22656	T22657	det	the,proteins
R6501	T22657	T22655	pobj	proteins,of
R6502	T22658	T22657	nmod	Pygo1,proteins
R6503	T22659	T22658	cc	and,Pygo1
R6504	T22660	T22658	conj	Pygo2,Pygo1
R6505	T22661	T22654	prep	in,patterns
R6506	T22662	T22663	det	the,cortex
R6507	T22663	T22661	pobj	cortex,in
R6508	T22664	T22663	prep	of,cortex
R6509	T22665	T22666	det	the,kidney
R651	T2910	T2884	punct	.,binds
R6510	T22666	T22664	pobj	kidney,of
R6511	T22667	T22666	compound	E18.5,kidney
R6512	T22668	T22649	punct	.,used
R6513	T22670	T22671	preconj	Both,Pygo1
R6514	T22671	T22672	nsubjpass	Pygo1,localized
R6515	T22673	T22671	cc	and,Pygo1
R6516	T22674	T22671	conj	Pygo2,Pygo1
R6517	T22675	T22676	punct	(,red
R6518	T22676	T22674	parataxis	red,Pygo2
R6519	T22677	T22676	punct	),red
R652	T2912	T2913	det	The,domain
R6520	T22678	T22672	auxpass	were,localized
R6521	T22679	T22672	prep	in,localized
R6522	T22680	T22681	det	the,nucleus
R6523	T22681	T22679	pobj	nucleus,in
R6524	T22682	T22672	punct	", ",localized
R6525	T22683	T22684	mark	as,expected
R6526	T22684	T22672	advcl	expected,localized
R6527	T22685	T22672	punct	.,localized
R6528	T22687	T22688	det	Both,genes
R6529	T22688	T22689	nsubj	genes,showed
R653	T2913	T2917	nsubjpass	domain,required
R6530	T22690	T22691	amod	widespread,expression
R6531	T22691	T22689	dobj	expression,showed
R6532	T22692	T22689	punct	", ",showed
R6533	T22693	T22689	prep	with,showed
R6534	T22694	T22693	pobj	signal,with
R6535	T22695	T22694	acl	detected,signal
R6536	T22696	T22695	prep	in,detected
R6537	T22697	T22698	det	all,components
R6538	T22698	T22696	pobj	components,in
R6539	T22699	T22698	prep	of,components
R654	T2914	T2915	npadvmod	N,terminal
R6540	T22700	T22701	det	the,kidney
R6541	T22701	T22699	pobj	kidney,of
R6542	T22702	T22701	amod	developing,kidney
R6543	T22703	T22693	punct	", ",with
R6544	T22704	T22693	cc	but,with
R6545	T22705	T22693	conj	with,with
R6546	T22706	T22707	amod	elevated,levels
R6547	T22707	T22705	pobj	levels,with
R6548	T22708	T22707	prep	in,levels
R6549	T22709	T22710	det	the,compartment
R655	T2915	T2913	amod	terminal,domain
R6550	T22710	T22708	pobj	compartment,in
R6551	T22711	T22712	amod	stromal,cell
R6552	T22712	T22710	compound	cell,compartment
R6553	T22713	T22714	punct	(,arrows
R6554	T22714	T22707	parataxis	arrows,levels
R6555	T22715	T22714	punct	),arrows
R6556	T22716	T22689	punct	.,showed
R6557	T22718	T22719	amod	Epithelial,cells
R6558	T22719	T22720	nsubjpass	cells,labeled
R6559	T22721	T22719	punct	", ",cells
R656	T2916	T2915	punct	-,terminal
R6560	T22722	T22723	advmod	primarily,bud
R6561	T22723	T22719	appos	bud,cells
R6562	T22724	T22723	amod	ureteric,bud
R6563	T22725	T22719	prep	in,cells
R6564	T22726	T22727	det	these,sections
R6565	T22727	T22725	pobj	sections,in
R6566	T22728	T22720	punct	", ",labeled
R6567	T22729	T22720	auxpass	were,labeled
R6568	T22730	T22720	oprd	blue,labeled
R6569	T22731	T22720	advcl	using,labeled
R657	T2918	T2913	prep	of,domain
R6570	T22732	T22733	compound	E,cadherin
R6571	T22733	T22735	compound	cadherin,antibody
R6572	T22734	T22733	punct	-,cadherin
R6573	T22735	T22731	dobj	antibody,using
R6574	T22736	T22720	punct	.,labeled
R6575	T22738	T22739	amod	Original,magnification
R6576	T22739	T22740	dep	magnification,200
R6577	T22741	T22740	punct	×,200
R6578	T22742	T22740	punct	.,200
R6579	T23117	T23118	amod	Confocal,analysis
R658	T2919	T2918	pobj	Pygo,of
R6580	T23119	T23118	prep	of,analysis
R6581	T23120	T23121	compound	Pygo1,Pygo2
R6582	T23121	T23123	compound	Pygo2,mutant
R6583	T23122	T23121	punct	/,Pygo2
R6584	T23123	T23124	compound	mutant,kidneys
R6585	T23124	T23119	pobj	kidneys,of
R6586	T23125	T23124	compound	E18.5,kidneys
R6587	T23126	T23118	punct	.,analysis
R6588	T23128	T23129	punct	(,B
R6589	T23129	T23130	meta	B,stained
R659	T2920	T2917	auxpass	is,required
R6590	T23131	T23129	punct	", ",B
R6591	T23132	T23129	dep	D,B
R6592	T23133	T23129	punct	),B
R6593	T23134	T23135	nmod	Pygo1,mutant
R6594	T23135	T23144	compound	mutant,kidneys
R6595	T23136	T23134	punct	-,Pygo1
R6596	T23137	T23134	punct	/,Pygo1
R6597	T23138	T23134	punct	-,Pygo1
R6598	T23139	T23134	punct	/,Pygo1
R6599	T23140	T23134	appos	Pygo2,Pygo1
R66	T1012	T986	punct	.,made
R660	T2921	T2917	prep	for,required
R6600	T23141	T23140	punct	-,Pygo2
R6601	T23142	T23140	punct	/,Pygo2
R6602	T23143	T23140	punct	-,Pygo2
R6603	T23144	T23130	nsubjpass	kidneys,stained
R6604	T23145	T23144	compound	E18.5,kidneys
R6605	T23146	T23144	cc	and,kidneys
R6606	T23147	T23148	punct	(,C
R6607	T23148	T23151	parataxis	C,kidneys
R6608	T23149	T23148	nmod	A,C
R6609	T23150	T23148	punct	", ",C
R661	T2922	T2923	nmod	Wnt,activation
R6610	T23151	T23144	conj	kidneys,kidneys
R6611	T23152	T23148	punct	),C
R6612	T23153	T23151	prep	of,kidneys
R6613	T23154	T23155	amod	normal,littermates
R6614	T23155	T23153	pobj	littermates,of
R6615	T23156	T23130	auxpass	were,stained
R6616	T23157	T23130	advcl	using,stained
R6617	T23158	T23157	dobj	antibodies,using
R6618	T23159	T23158	prep	to,antibodies
R6619	T23160	T23161	punct	(,D
R662	T2923	T2921	pobj	activation,for
R6620	T23161	T23164	parataxis	D,Cited1
R6621	T23162	T23161	nmod	C,D
R6622	T23163	T23161	punct	", ",D
R6623	T23164	T23159	pobj	Cited1,to
R6624	T23165	T23161	punct	),D
R6625	T23166	T23164	cc	or,Cited1
R6626	T23167	T23168	punct	(,B
R6627	T23168	T23171	parataxis	B,Wt1
R6628	T23169	T23168	nmod	A,B
R6629	T23170	T23168	punct	", ",B
R663	T2924	T2923	amod	transcriptional,activation
R6630	T23171	T23164	conj	Wt1,Cited1
R6631	T23172	T23168	punct	),B
R6632	T23173	T23164	punct	", ",Cited1
R6633	T23174	T23175	advmod	both,expressed
R6634	T23175	T23164	acl	expressed,Cited1
R6635	T23176	T23175	prep	in,expressed
R6636	T23177	T23178	det	the,mesenchyme
R6637	T23178	T23176	pobj	mesenchyme,in
R6638	T23179	T23178	amod	condensing,mesenchyme
R6639	T23180	T23178	amod	metanephric,mesenchyme
R664	T2925	T2917	punct	", ",required
R6640	T23181	T23175	punct	", ",expressed
R6641	T23182	T23175	cc	and,expressed
R6642	T23183	T23175	conj	colored,expressed
R6643	T23184	T23183	prep	as,colored
R6644	T23185	T23184	pobj	red,as
R6645	T23186	T23164	punct	", ",Cited1
R6646	T23187	T23164	conj	Cdh1,Cited1
R6647	T23188	T23187	punct	", ",Cdh1
R6648	T23189	T23190	det	a,marker
R6649	T23190	T23187	appos	marker,Cdh1
R665	T2926	T2927	mark	while,required
R6650	T23191	T23190	amod	general,marker
R6651	T23192	T23190	prep	of,marker
R6652	T23193	T23192	pobj	epithelia,of
R6653	T23194	T23195	punct	(,blue
R6654	T23195	T23187	parataxis	blue,Cdh1
R6655	T23196	T23195	punct	),blue
R6656	T23197	T23187	punct	", ",Cdh1
R6657	T23198	T23187	cc	and,Cdh1
R6658	T23199	T23200	compound	DBA,lectin
R6659	T23200	T23187	conj	lectin,Cdh1
R666	T2927	T2917	advcl	required,required
R6660	T23201	T23200	acl	staining,lectin
R6661	T23202	T23203	det	the,tree
R6662	T23203	T23201	dobj	tree,staining
R6663	T23204	T23203	amod	ureteric,tree
R6664	T23205	T23206	punct	[,16
R6665	T23206	T23201	parataxis	16,staining
R6666	T23207	T23206	punct	],16
R6667	T23208	T23130	punct	.,stained
R6668	T23210	T23211	amod	Confocal,sections
R6669	T23211	T23214	nsubjpass	sections,obtained
R667	T2928	T2929	det	the,motif
R6670	T23212	T23211	compound	Z,sections
R6671	T23213	T23211	punct	-,sections
R6672	T23215	T23214	auxpass	were,obtained
R6673	T23216	T23217	det	every,μm
R6674	T23217	T23214	dobj	μm,obtained
R6675	T23218	T23217	nummod	5,μm
R6676	T23219	T23214	prep	for,obtained
R6677	T23220	T23221	quantmod	75,80
R6678	T23221	T23223	nummod	80,μm
R6679	T23222	T23221	punct	–,80
R668	T2929	T2927	nsubjpass	motif,required
R6680	T23223	T23219	pobj	μm,for
R6681	T23224	T23214	punct	.,obtained
R6682	T23226	T23227	det	A,number
R6683	T23227	T23229	nsubjpass	number,observed
R6684	T23228	T23227	amod	reduced,number
R6685	T23230	T23227	prep	of,number
R6686	T23231	T23232	amod	ureteric,tips
R6687	T23232	T23230	pobj	tips,of
R6688	T23233	T23232	prep	per,tips
R6689	T23234	T23233	pobj	area,per
R669	T2930	T2929	compound	PHD,motif
R6690	T23235	T23229	auxpass	was,observed
R6691	T23236	T23229	prep	in,observed
R6692	T23237	T23238	punct	(,D
R6693	T23238	T23241	parataxis	D,kidneys
R6694	T23239	T23238	nmod	B,D
R6695	T23240	T23238	punct	", ",D
R6696	T23241	T23236	pobj	kidneys,in
R6697	T23242	T23238	punct	),D
R6698	T23243	T23241	det	the,kidneys
R6699	T23244	T23245	compound	Pygo1,Pygo2
R67	T1016	T1017	det	Each,mutation
R670	T2931	T2927	auxpass	is,required
R6700	T23245	T23247	npadvmod	Pygo2,null
R6701	T23246	T23245	punct	/,Pygo2
R6702	T23247	T23241	amod	null,kidneys
R6703	T23248	T23229	prep	compared,observed
R6704	T23249	T23248	prep	with,compared
R6705	T23250	T23251	punct	(,C
R6706	T23251	T23254	parataxis	C,littermates
R6707	T23252	T23251	nmod	A,C
R6708	T23253	T23251	punct	", ",C
R6709	T23254	T23249	pobj	littermates,with
R671	T2932	T2927	prep	for,required
R6710	T23255	T23251	punct	),C
R6711	T23256	T23254	compound	control,littermates
R6712	T23257	T23229	punct	.,observed
R6713	T23259	T23260	prep	In,were
R6714	T23261	T23259	pobj	addition,In
R6715	T23262	T23260	punct	", ",were
R6716	T23263	T23264	amod	ureteric,bud
R6717	T23264	T23265	compound	bud,tips
R6718	T23265	T23260	nsubj	tips,were
R6719	T23266	T23260	advmod	often,were
R672	T2933	T2934	det	the,association
R6720	T23267	T23268	advmod	more,dilated
R6721	T23268	T23260	acomp	dilated,were
R6722	T23269	T23260	prep	in,were
R6723	T23270	T23271	npadvmod	Pygo1,null
R6724	T23271	T23274	amod	null,mutants
R6725	T23272	T23270	punct	/,Pygo1
R6726	T23273	T23270	nummod	2,Pygo1
R6727	T23274	T23269	pobj	mutants,in
R6728	T23275	T23260	prep	compared,were
R6729	T23276	T23275	prep	with,compared
R673	T2934	T2932	pobj	association,for
R6730	T23277	T23276	pobj	controls,with
R6731	T23278	T23260	punct	.,were
R6732	T23280	T23281	det	The,mesenchyme
R6733	T23281	T23283	nsubjpass	mesenchyme,expanded
R6734	T23282	T23281	amod	condensed,mesenchyme
R6735	T23284	T23281	acl	surrounding,mesenchyme
R6736	T23285	T23286	det	the,bud
R6737	T23286	T23284	dobj	bud,surrounding
R6738	T23287	T23286	amod	ureteric,bud
R6739	T23288	T23283	auxpass	was,expanded
R674	T2935	T2934	prep	of,association
R6740	T23289	T23283	advmod	also,expanded
R6741	T23290	T23283	advmod	significantly,expanded
R6742	T23291	T23283	prep	in,expanded
R6743	T23292	T23293	det	the,mutants
R6744	T23293	T23291	pobj	mutants,in
R6745	T23294	T23283	punct	.,expanded
R6746	T23296	T23297	amod	Original,magnification
R6747	T23297	T23298	dep	magnification,200
R6748	T23299	T23298	punct	×,200
R6749	T23300	T23298	punct	.,200
R675	T2936	T2935	pobj	Pygo,of
R6750	T23567	T23566	prep	of,Expression
R6751	T23568	T23567	pobj	Wnt7b,of
R6752	T23569	T23568	punct	", ",Wnt7b
R6753	T23570	T23568	conj	Wnt9b,Wnt7b
R6754	T23571	T23570	punct	", ",Wnt9b
R6755	T23572	T23570	cc	and,Wnt9b
R6756	T23573	T23570	conj	Wnt11,Wnt9b
R6757	T23574	T23566	prep	in,Expression
R6758	T23575	T23576	nmod	E18.5,kidneys
R6759	T23576	T23574	pobj	kidneys,in
R676	T2937	T2934	prep	with,association
R6760	T23577	T23578	compound	Pygo1,Pygo2
R6761	T23578	T23580	npadvmod	Pygo2,null
R6762	T23579	T23578	punct	/,Pygo2
R6763	T23580	T23576	amod	null,kidneys
R6764	T23581	T23580	npadvmod	compound,null
R6765	T23582	T23566	punct	.,Expression
R6766	T23584	T23585	amod	Whole,mount
R6767	T23586	T23585	punct	-,mount
R6768	T23587	T23585	nmod	in,mount
R6769	T23588	T23585	nmod	situ,mount
R677	T2938	T2937	pobj	Lgs,with
R6770	T23589	T23585	prep	of,mount
R6771	T23590	T23589	pobj	hybridizations,of
R6772	T23591	T23590	prep	of,hybridizations
R6773	T23592	T23593	punct	(,A
R6774	T23593	T23594	parataxis	A,kidneys
R6775	T23594	T23591	pobj	kidneys,of
R6776	T23595	T23596	punct	-,C
R6777	T23596	T23593	prep	C,A
R6778	T23597	T23593	punct	),A
R6779	T23598	T23594	nmod	E18.5,kidneys
R678	T2939	T2940	punct	[,13
R6780	T23599	T23600	amod	wild,type
R6781	T23600	T23594	compound	type,kidneys
R6782	T23601	T23600	punct	-,type
R6783	T23602	T23594	cc	and,kidneys
R6784	T23603	T23604	punct	(,D
R6785	T23604	T23605	parataxis	D,kidneys
R6786	T23605	T23594	conj	kidneys,kidneys
R6787	T23606	T23607	punct	-,F
R6788	T23607	T23604	prep	F,D
R6789	T23608	T23604	punct	),D
R679	T2940	T2927	parataxis	13,required
R6790	T23609	T23610	nmod	Pygo1,mutant
R6791	T23610	T23605	compound	mutant,kidneys
R6792	T23611	T23609	punct	-,Pygo1
R6793	T23612	T23609	punct	/,Pygo1
R6794	T23613	T23609	punct	-,Pygo1
R6795	T23614	T23609	punct	/,Pygo1
R6796	T23615	T23609	appos	Pygo2,Pygo1
R6797	T23616	T23615	punct	-,Pygo2
R6798	T23617	T23615	punct	/,Pygo2
R6799	T23618	T23615	punct	-,Pygo2
R68	T1017	T1018	nsubj	mutation,resulted
R680	T2941	T2940	nummod	9,13
R6800	T23619	T23590	prep	with,hybridizations
R6801	T23620	T23621	det	the,markers
R6802	T23621	T23619	pobj	markers,with
R6803	T23622	T23623	amod	ureteric,bud
R6804	T23623	T23624	npadvmod	bud,derivative
R6805	T23624	T23621	amod	derivative,markers
R6806	T23625	T23621	punct	: ,markers
R6807	T23626	T23627	punct	(,D
R6808	T23627	T23630	parataxis	D,Wnt7b
R6809	T23628	T23627	nmod	A,D
R681	T2942	T2940	punct	",",13
R6810	T23629	T23627	punct	", ",D
R6811	T23630	T23621	appos	Wnt7b,markers
R6812	T23631	T23627	punct	),D
R6813	T23632	T23630	punct	", ",Wnt7b
R6814	T23633	T23634	punct	(,E
R6815	T23634	T23637	parataxis	E,Wnt9b
R6816	T23635	T23634	nmod	B,E
R6817	T23636	T23634	punct	", ",E
R6818	T23637	T23630	conj	Wnt9b,Wnt7b
R6819	T23638	T23634	punct	),E
R682	T2943	T2940	punct	],13
R6820	T23639	T23637	punct	", ",Wnt9b
R6821	T23640	T23637	cc	and,Wnt9b
R6822	T23641	T23642	punct	(,F
R6823	T23642	T23645	parataxis	F,Wnt11
R6824	T23643	T23642	nmod	C,F
R6825	T23644	T23642	punct	", ",F
R6826	T23645	T23637	conj	Wnt11,Wnt9b
R6827	T23646	T23642	punct	),F
R6828	T23647	T23585	punct	.,mount
R6829	T23649	T23650	det	The,kidneys
R683	T2944	T2917	punct	.,required
R6830	T23650	T23652	nsubj	kidneys,showed
R6831	T23651	T23650	compound	mutant,kidneys
R6832	T23653	T23654	amod	normal,patterns
R6833	T23654	T23652	dobj	patterns,showed
R6834	T23655	T23654	compound	expression,patterns
R6835	T23656	T23654	prep	for,patterns
R6836	T23657	T23658	det	the,markers
R6837	T23658	T23656	pobj	markers,for
R6838	T23659	T23660	amod	ureteric,stalk
R6839	T23660	T23658	compound	stalk,markers
R684	T2946	T2947	advmod	Additionally,identified
R6840	T23661	T23658	appos	Wnt7b,markers
R6841	T23662	T23661	cc	and,Wnt7b
R6842	T23663	T23661	conj	Wnt9b,Wnt7b
R6843	T23664	T23654	punct	", ",patterns
R6844	T23665	T23654	cc	and,patterns
R6845	T23666	T23667	amod	reduced,density
R6846	T23667	T23654	conj	density,patterns
R6847	T23668	T23667	prep	of,density
R6848	T23669	T23670	amod	ureteric,tips
R6849	T23670	T23668	pobj	tips,of
R685	T2948	T2947	punct	", ",identified
R6850	T23671	T23672	mark	as,measured
R6851	T23672	T23652	advcl	measured,showed
R6852	T23673	T23672	agent	by,measured
R6853	T23674	T23673	pobj	Wnt11,by
R6854	T23675	T23652	punct	.,showed
R6855	T23677	T23678	amod	Original,magnification
R6856	T23678	T23679	dep	magnification,32
R6857	T23680	T23679	punct	×,32
R6858	T23681	T23679	punct	.,32
R686	T2949	T2950	det	a,signal
R6861	T24238	T24239	amod	Reduced,signaling
R6862	T24240	T24239	amod	canonical,signaling
R6863	T24241	T24239	compound	Wnt,signaling
R6864	T24242	T24239	prep	in,signaling
R6865	T24243	T24244	nmod	Pygo2,mutant
R6866	T24244	T24249	compound	mutant,embryos
R6867	T24245	T24243	cc	and,Pygo2
R6868	T24246	T24247	compound	Pygo1,Pygo2
R6869	T24247	T24243	conj	Pygo2,Pygo2
R687	T2950	T2947	nsubjpass	signal,identified
R6870	T24248	T24247	punct	/,Pygo2
R6871	T24249	T24242	pobj	embryos,in
R6872	T24250	T24239	punct	.,signaling
R6873	T24252	T24253	compound	E10.5,embryos
R6874	T24254	T24253	punct	", ",embryos
R6875	T24255	T24253	appos	all,embryos
R6876	T24256	T24255	prep	with,all
R6877	T24257	T24258	det	the,reporter
R6878	T24258	T24256	pobj	reporter,with
R6879	T24259	T24260	compound	BAT,gal
R688	T2951	T2950	amod	putative,signal
R6880	T24260	T24262	compound	gal,transgene
R6881	T24261	T24260	punct	-,gal
R6882	T24262	T24258	compound	transgene,reporter
R6883	T24263	T24258	prep	of,reporter
R6884	T24264	T24265	amod	canonical,signaling
R6885	T24265	T24263	pobj	signaling,of
R6886	T24266	T24265	compound	Wnt,signaling
R6887	T24267	T24253	punct	.,embryos
R6888	T24269	T24270	punct	(,A
R6889	T24270	T24271	meta	A,showed
R689	T2952	T2950	amod	nuclear,signal
R6890	T24272	T24270	punct	),A
R6891	T24273	T24274	nmod	Pygo1,embryos
R6892	T24274	T24271	nsubj	embryos,showed
R6893	T24275	T24273	punct	+,Pygo1
R6894	T24276	T24273	punct	/,Pygo1
R6895	T24277	T24273	punct	+,Pygo1
R6896	T24278	T24273	punct	/,Pygo1
R6897	T24279	T24273	appos	Pygo2,Pygo1
R6898	T24280	T24279	punct	+,Pygo2
R6899	T24281	T24279	punct	/,Pygo2
R69	T1019	T1018	aux	deleted,resulted
R690	T2953	T2950	compound	localization,signal
R6900	T24282	T24279	punct	-,Pygo2
R6901	T24283	T24284	amod	normal,staining
R6902	T24284	T24271	dobj	staining,showed
R6903	T24285	T24286	compound	X,gal
R6904	T24286	T24284	compound	gal,staining
R6905	T24287	T24286	punct	-,gal
R6906	T24288	T24271	prep	in,showed
R6907	T24289	T24290	det	the,brain
R6908	T24290	T24288	pobj	brain,in
R6909	T24291	T24290	amod	developing,brain
R691	T2954	T2950	punct	(,signal
R6910	T24292	T24290	punct	", ",brain
R6911	T24293	T24294	amod	pharyngeal,pouches
R6912	T24294	T24290	appos	pouches,brain
R6913	T24295	T24290	punct	", ",brain
R6914	T24296	T24297	amod	otic,vesicle
R6915	T24297	T24290	appos	vesicle,brain
R6916	T24298	T24290	punct	", ",brain
R6917	T24299	T24300	amod	apical,ridges
R6918	T24300	T24290	appos	ridges,brain
R6919	T24301	T24300	amod	ectodermal,ridges
R692	T2955	T2950	appos	NLS,signal
R6920	T24302	T24300	prep	of,ridges
R6921	T24303	T24304	det	the,buds
R6922	T24304	T24302	pobj	buds,of
R6923	T24305	T24306	amod	fore,limb
R6924	T24306	T24304	compound	limb,buds
R6925	T24307	T24305	cc	and,fore
R6926	T24308	T24305	conj	hind,fore
R6927	T24309	T24288	punct	", ",in
R6928	T24310	T24288	cc	and,in
R6929	T24311	T24288	conj	in,in
R693	T2956	T2947	punct	),identified
R6930	T24312	T24311	pobj	somites,in
R6931	T24313	T24271	punct	.,showed
R6932	T24315	T24316	punct	(,B
R6933	T24316	T24317	meta	B,showed
R6934	T24318	T24316	punct	),B
R6935	T24319	T24320	nmod	Pygo1,embryos
R6936	T24320	T24317	nsubj	embryos,showed
R6937	T24321	T24319	punct	+,Pygo1
R6938	T24322	T24319	punct	/,Pygo1
R6939	T24323	T24319	punct	-,Pygo1
R694	T2957	T2947	auxpass	was,identified
R6940	T24324	T24319	punct	/,Pygo1
R6941	T24325	T24319	appos	Pygo2,Pygo1
R6942	T24326	T24325	punct	-,Pygo2
R6943	T24327	T24325	punct	/,Pygo2
R6944	T24328	T24325	punct	-,Pygo2
R6945	T24329	T24320	punct	", ",embryos
R6946	T24330	T24320	prep	with,embryos
R6947	T24331	T24330	pobj	loss,with
R6948	T24332	T24331	prep	of,loss
R6949	T24333	T24334	det	both,alleles
R695	T2958	T2947	prep	within,identified
R6950	T24334	T24332	pobj	alleles,of
R6951	T24335	T24334	compound	Pygo2,alleles
R6952	T24336	T24317	punct	", ",showed
R6953	T24337	T24338	amod	reduced,reporter
R6954	T24338	T24345	compound	reporter,expression
R6955	T24339	T24337	cc	but,reduced
R6956	T24340	T24339	neg	not,but
R6957	T24341	T24337	conj	absent,reduced
R6958	T24342	T24343	compound	BAT,gal
R6959	T24343	T24338	compound	gal,reporter
R696	T2959	T2960	det	the,domain
R6960	T24344	T24343	punct	-,gal
R6961	T24345	T24317	dobj	expression,showed
R6962	T24346	T24317	prep	in,showed
R6963	T24347	T24348	amod	many,structures
R6964	T24348	T24346	pobj	structures,in
R6965	T24349	T24348	amod	developing,structures
R6966	T24350	T24348	punct	", ",structures
R6967	T24351	T24348	prep	including,structures
R6968	T24352	T24353	amod	pharyngeal,pouches
R6969	T24353	T24351	pobj	pouches,including
R697	T2960	T2958	pobj	domain,within
R6970	T24354	T24353	punct	", ",pouches
R6971	T24355	T24356	amod	otic,vesicle
R6972	T24356	T24353	conj	vesicle,pouches
R6973	T24357	T24356	punct	", ",vesicle
R6974	T24358	T24356	cc	and,vesicle
R6975	T24359	T24356	conj	somites,vesicle
R6976	T24360	T24317	punct	.,showed
R6977	T24362	T24363	punct	(,C
R6978	T24363	T24364	meta	C,showed
R6979	T24365	T24363	punct	),C
R698	T2961	T2962	npadvmod	N,terminal
R6980	T24366	T24367	nmod	Pygo1,embryo
R6981	T24367	T24364	nsubj	embryo,showed
R6982	T24368	T24366	punct	-,Pygo1
R6983	T24369	T24366	punct	/,Pygo1
R6984	T24370	T24366	punct	-,Pygo1
R6985	T24371	T24366	punct	/,Pygo1
R6986	T24372	T24366	appos	Pygo2,Pygo1
R6987	T24373	T24372	punct	+,Pygo2
R6988	T24374	T24372	punct	/,Pygo2
R6989	T24375	T24372	punct	-,Pygo2
R699	T2962	T2960	amod	terminal,domain
R6990	T24376	T24367	punct	", ",embryo
R6991	T24377	T24367	prep	with,embryo
R6992	T24378	T24377	pobj	mutation,with
R6993	T24379	T24378	prep	of,mutation
R6994	T24380	T24381	det	both,alleles
R6995	T24381	T24379	pobj	alleles,of
R6996	T24382	T24381	compound	Pygo1,alleles
R6997	T24383	T24381	punct	", ",alleles
R6998	T24384	T24381	cc	but,alleles
R6999	T24385	T24386	nummod	one,allele
R7	T946	T943	prep	in,roles
R70	T1020	T1021	punct	>,80
R700	T2963	T2962	punct	-,terminal
R7000	T24386	T24381	conj	allele,alleles
R7001	T24387	T24388	amod	wild,type
R7002	T24388	T24386	compound	type,allele
R7003	T24389	T24388	punct	-,type
R7004	T24390	T24386	compound	Pygo2,allele
R7005	T24391	T24364	punct	", ",showed
R7006	T24392	T24393	amod	normal,expression
R7007	T24393	T24364	dobj	expression,showed
R7008	T24394	T24395	compound	BAT,gal
R7009	T24395	T24393	compound	gal,expression
R701	T2964	T2960	prep	of,domain
R7010	T24396	T24395	punct	-,gal
R7011	T24397	T24364	punct	.,showed
R7012	T24399	T24400	punct	(,D
R7013	T24400	T24401	meta	D,showed
R7014	T24402	T24400	punct	),D
R7015	T24403	T24404	amod	Double,homozygous
R7016	T24404	T24406	amod	homozygous,embryos
R7017	T24405	T24404	punct	-,homozygous
R7018	T24406	T24401	nsubj	embryos,showed
R7019	T24407	T24406	nmod	mutant,embryos
R702	T2965	T2964	pobj	Pygo,of
R7020	T24408	T24406	nmod	Pygo1,embryos
R7021	T24409	T24408	punct	-,Pygo1
R7022	T24410	T24408	punct	/,Pygo1
R7023	T24411	T24408	punct	-,Pygo1
R7024	T24412	T24408	punct	/,Pygo1
R7025	T24413	T24408	appos	Pygo2,Pygo1
R7026	T24414	T24413	punct	-,Pygo2
R7027	T24415	T24413	punct	/,Pygo2
R7028	T24416	T24413	punct	-,Pygo2
R7029	T24417	T24401	advmod	still,showed
R703	T2966	T2947	punct	", ",identified
R7030	T24418	T24419	det	some,expression
R7031	T24419	T24401	dobj	expression,showed
R7032	T24420	T24419	amod	remaining,expression
R7033	T24421	T24422	compound	BAT,gal
R7034	T24422	T24419	compound	gal,expression
R7035	T24423	T24422	punct	-,gal
R7036	T24424	T24401	punct	", ",showed
R7037	T24425	T24401	advcl	suggesting,showed
R7038	T24426	T24427	amod	residual,signaling
R7039	T24427	T24425	dobj	signaling,suggesting
R704	T2967	T2947	advcl	suggesting,identified
R7040	T24428	T24427	amod	canonical,signaling
R7041	T24429	T24427	compound	Wnt,signaling
R7042	T24430	T24401	punct	.,showed
R7043	T24432	T24433	nsubj	Embryos,were
R7044	T24434	T24432	prep	in,Embryos
R7045	T24435	T24436	nmod	panels,A
R7046	T24436	T24434	pobj	A,in
R7047	T24437	T24436	punct	(,A
R7048	T24438	T24436	punct	),A
R7049	T24439	T24436	cc	and,A
R705	T2968	T2969	det	a,role
R7050	T24440	T24441	punct	(,B
R7051	T24441	T24436	conj	B,A
R7052	T24442	T24433	punct	),were
R7053	T24443	T24433	prep	from,were
R7054	T24444	T24445	det	the,litter
R7055	T24445	T24443	pobj	litter,from
R7056	T24446	T24445	amod	same,litter
R7057	T24447	T24433	cc	and,were
R7058	T24448	T24449	auxpass	were,processed
R7059	T24449	T24433	conj	processed,were
R706	T2969	T2967	dobj	role,suggesting
R7060	T24450	T24449	prep	in,processed
R7061	T24451	T24450	pobj	parallel,in
R7062	T24452	T24449	punct	", ",processed
R7063	T24453	T24454	mark	while,were
R7064	T24454	T24449	advcl	were,processed
R7065	T24455	T24454	nsubj	embryos,were
R7066	T24456	T24455	prep	in,embryos
R7067	T24457	T24456	punct	(,in
R7068	T24458	T24456	pobj	C,in
R7069	T24459	T24458	punct	),C
R707	T2970	T2969	amod	possible,role
R7070	T24460	T24458	cc	and,C
R7071	T24461	T24462	punct	(,D
R7072	T24462	T24458	conj	D,C
R7073	T24463	T24454	punct	),were
R7074	T24464	T24454	prep	from,were
R7075	T24465	T24466	det	a,litter
R7076	T24466	T24464	pobj	litter,from
R7077	T24467	T24466	amod	separate,litter
R7078	T24468	T24466	punct	", ",litter
R7079	T24469	T24470	advmod	also,processed
R708	T2971	T2969	prep	of,role
R7080	T24470	T24466	acl	processed,litter
R7081	T24471	T24470	prep	in,processed
R7082	T24472	T24471	pobj	parallel,in
R7083	T24473	T24454	punct	", ",were
R7084	T24474	T24454	cc	and,were
R7085	T24475	T24454	conj	were,were
R7086	T24476	T24477	advmod	slightly,more
R7087	T24477	T24478	advmod	more,advanced
R7088	T24478	T24475	acomp	advanced,were
R7089	T24479	T24478	advmod	developmentally,advanced
R709	T2972	T2973	amod	nuclear,importation
R7090	T24480	T24433	punct	.,were
R7091	T24482	T24483	amod	Original,magnification
R7092	T24483	T24484	dep	magnification,20
R7093	T24485	T24486	punct	(,B
R7094	T24486	T24484	parataxis	B,20
R7095	T24487	T24486	nmod	A,B
R7096	T24488	T24486	punct	", ",B
R7097	T24489	T24486	punct	),B
R7098	T24490	T24484	punct	×,20
R7099	T24491	T24484	punct	;,20
R71	T1021	T1022	nummod	80,%
R710	T2973	T2971	pobj	importation,of
R7100	T24492	T24493	punct	(,D
R7101	T24493	T24496	parataxis	D,16
R7102	T24494	T24493	nmod	C,D
R7103	T24495	T24493	punct	", ",D
R7104	T24496	T24484	appos	16,20
R7105	T24497	T24493	punct	),D
R7106	T24498	T24496	punct	×,16
R7107	T24499	T24484	punct	.,20
R7108	T25934	T25935	nsubjpass	Pygo2,required
R7109	T25936	T25935	auxpass	is,required
R711	T2974	T2973	prep	of,importation
R7110	T25937	T25935	prep	for,required
R7111	T25938	T25939	compound	BAT,gal
R7112	T25939	T25941	compound	gal,reporter
R7113	T25940	T25939	punct	-,gal
R7114	T25941	T25942	compound	reporter,expression
R7115	T25942	T25937	pobj	expression,for
R7116	T25943	T25935	prep	in,required
R7117	T25944	T25945	amod	ureteric,bud
R7118	T25945	T25946	npadvmod	bud,derived
R7119	T25946	T25948	amod	derived,structures
R712	T2975	T2976	compound	β,catenin
R7120	T25947	T25946	punct	-,derived
R7121	T25948	T25943	pobj	structures,in
R7122	T25949	T25948	prep	of,structures
R7123	T25950	T25951	det	the,kidney
R7124	T25951	T25949	pobj	kidney,of
R7125	T25952	T25951	amod	developing,kidney
R7126	T25953	T25935	punct	.,required
R7127	T25955	T25956	compound	X,Gal
R7128	T25956	T25958	compound	Gal,staining
R7129	T25957	T25956	punct	-,Gal
R713	T2976	T2974	pobj	catenin,of
R7130	T25959	T25958	prep	of,staining
R7131	T25960	T25961	compound	BAT,gal
R7132	T25961	T25963	npadvmod	gal,transgenic
R7133	T25962	T25961	punct	-,gal
R7134	T25963	T25964	amod	transgenic,tracts
R7135	T25964	T25959	pobj	tracts,of
R7136	T25965	T25966	punct	(,A
R7137	T25966	T25967	parataxis	A,E10.5
R7138	T25967	T25964	nmod	E10.5,tracts
R7139	T25968	T25969	punct	-,C
R714	T2977	T2976	punct	-,catenin
R7140	T25969	T25966	prep	C,A
R7141	T25970	T25966	punct	),A
R7142	T25971	T25967	punct	", ",E10.5
R7143	T25972	T25967	cc	and,E10.5
R7144	T25973	T25974	punct	(,E
R7145	T25974	T25975	parataxis	E,E13.5
R7146	T25975	T25967	conj	E13.5,E10.5
R7147	T25976	T25977	punct	-,L
R7148	T25977	T25974	prep	L,E
R7149	T25978	T25974	punct	),E
R715	T2978	T2979	punct	[,11
R7150	T25979	T25964	amod	urogenital,tracts
R7151	T25980	T25964	punct	", ",tracts
R7152	T25981	T25964	cc	and,tracts
R7153	T25982	T25983	punct	(,M
R7154	T25983	T25984	parataxis	M,kidneys
R7155	T25984	T25964	conj	kidneys,tracts
R7156	T25985	T25986	punct	-,O
R7157	T25986	T25983	prep	O,M
R7158	T25987	T25983	punct	),M
R7159	T25988	T25984	compound	E18.5,kidneys
R716	T2979	T2947	parataxis	11,identified
R7160	T25989	T25958	punct	.,staining
R7161	T25991	T25992	punct	(,A
R7162	T25992	T25993	meta	A,Pygo1
R7163	T25994	T25992	punct	),A
R7164	T25995	T25993	punct	-,Pygo1
R7165	T25996	T25993	punct	/,Pygo1
R7166	T25997	T25993	punct	-,Pygo1
R7167	T25998	T25993	punct	/,Pygo1
R7168	T25999	T25993	appos	Pygo2,Pygo1
R7169	T26000	T25999	punct	+,Pygo2
R717	T2980	T2979	nummod	8,11
R7170	T26001	T25999	punct	/,Pygo2
R7171	T26002	T25999	punct	+,Pygo2
R7172	T26003	T26004	punct	(,left
R7173	T26004	T25993	parataxis	left,Pygo1
R7174	T26005	T26004	punct	),left
R7175	T26006	T25993	cc	and,Pygo1
R7176	T26007	T25993	conj	Pygo1,Pygo1
R7177	T26008	T26007	punct	+,Pygo1
R7178	T26009	T26007	punct	/,Pygo1
R7179	T26010	T26007	punct	-,Pygo1
R718	T2981	T2979	punct	",",11
R7180	T26011	T26007	punct	/,Pygo1
R7181	T26012	T26007	appos	Pygo2,Pygo1
R7182	T26013	T26012	punct	-,Pygo2
R7183	T26014	T26012	punct	/,Pygo2
R7184	T26015	T26012	punct	-,Pygo2
R7185	T26016	T26017	punct	(,right
R7186	T26017	T26007	parataxis	right,Pygo1
R7187	T26018	T26017	punct	),right
R7188	T26019	T26007	punct	.,Pygo1
R7189	T26022	T26023	det	the,loss
R719	T2982	T2979	punct	],11
R7190	T26023	T26021	dobj	loss,Note
R7191	T26024	T26023	prep	of,loss
R7192	T26025	T26026	compound	reporter,activity
R7193	T26026	T26024	pobj	activity,of
R7194	T26027	T26023	prep	in,loss
R7195	T26028	T26029	det	the,duct
R7196	T26029	T26027	pobj	duct,in
R7197	T26030	T26029	amod	nephric,duct
R7198	T26031	T26032	punct	(,arrowhead
R7199	T26032	T26023	parataxis	arrowhead,loss
R72	T1022	T1018	dobj	%,resulted
R720	T2983	T2947	punct	.,identified
R7200	T26033	T26032	amod	black,arrowhead
R7201	T26034	T26032	punct	),arrowhead
R7202	T26035	T26023	cc	and,loss
R7203	T26036	T26023	conj	reduction,loss
R7204	T26037	T26036	prep	of,reduction
R7205	T26038	T26037	pobj	staining,of
R7206	T26039	T26036	prep	in,reduction
R7207	T26040	T26041	det	the,bud
R7208	T26041	T26039	pobj	bud,in
R7209	T26042	T26041	amod	ureteric,bud
R721	T2985	T2986	nsubj	Analyses,indicate
R7210	T26043	T26044	punct	(,arrow
R7211	T26044	T26041	parataxis	arrow,bud
R7212	T26045	T26044	amod	white,arrow
R7213	T26046	T26044	punct	),arrow
R7214	T26047	T26041	prep	of,bud
R7215	T26048	T26049	det	the,embryo
R7216	T26049	T26047	pobj	embryo,of
R7217	T26050	T26051	npadvmod	Pygo2,null
R7218	T26051	T26049	amod	null,embryo
R7219	T26052	T26053	punct	(,Right
R722	T2987	T2985	prep	of,Analyses
R7220	T26053	T26036	parataxis	Right,reduction
R7221	T26054	T26053	punct	),Right
R7222	T26055	T26021	punct	.,Note
R7223	T26057	T26058	punct	(,B
R7224	T26058	T26059	meta	B,embryo
R7225	T26060	T26058	punct	),B
R7226	T26061	T26059	nmod	Pygo1,embryo
R7227	T26062	T26061	punct	-,Pygo1
R7228	T26063	T26061	punct	/,Pygo1
R7229	T26064	T26061	punct	-,Pygo1
R723	T2988	T2989	amod	multiple,aspects
R7230	T26065	T26061	punct	/,Pygo1
R7231	T26066	T26061	appos	Pygo2,Pygo1
R7232	T26067	T26066	punct	+,Pygo2
R7233	T26068	T26066	punct	/,Pygo2
R7234	T26069	T26066	punct	-,Pygo2
R7235	T26070	T26059	compound	E10.5,embryo
R7236	T26071	T26059	prep	with,embryo
R7237	T26072	T26073	compound	BAT,gal
R7238	T26073	T26075	compound	gal,reporter
R7239	T26074	T26073	punct	-,gal
R724	T2989	T2987	pobj	aspects,of
R7240	T26075	T26076	compound	reporter,activity
R7241	T26076	T26071	pobj	activity,with
R7242	T26077	T26076	prep	in,activity
R7243	T26078	T26079	det	the,duct
R7244	T26079	T26077	pobj	duct,in
R7245	T26080	T26079	amod	nephric,duct
R7246	T26081	T26082	punct	(,arrowhead
R7247	T26082	T26079	parataxis	arrowhead,duct
R7248	T26083	T26082	amod	black,arrowhead
R7249	T26084	T26082	punct	),arrowhead
R725	T2990	T2989	prep	of,aspects
R7250	T26085	T26079	cc	and,duct
R7251	T26086	T26087	amod	ureteric,bud
R7252	T26087	T26079	conj	bud,duct
R7253	T26088	T26089	punct	(,arrow
R7254	T26089	T26087	parataxis	arrow,bud
R7255	T26090	T26089	amod	white,arrow
R7256	T26091	T26089	punct	),arrow
R7257	T26092	T26059	punct	.,embryo
R7258	T26094	T26095	punct	(,C
R7259	T26095	T26096	meta	C,embryo
R726	T2991	T2992	det	the,phenotype
R7260	T26097	T26095	punct	),C
R7261	T26098	T26096	nmod	Pygo1,embryo
R7262	T26099	T26098	punct	-,Pygo1
R7263	T26100	T26098	punct	/,Pygo1
R7264	T26101	T26098	punct	-,Pygo1
R7265	T26102	T26098	punct	/,Pygo1
R7266	T26103	T26098	appos	Pygo,Pygo1
R7267	T26104	T26103	punct	-,Pygo
R7268	T26105	T26103	punct	/,Pygo
R7269	T26106	T26103	punct	-,Pygo
R727	T2992	T2990	pobj	phenotype,of
R7270	T26107	T26096	punct	", ",embryo
R7271	T26108	T26109	mark	with,lost
R7272	T26109	T26096	advcl	lost,embryo
R7273	T26110	T26111	compound	reporter,expression
R7274	T26111	T26109	nsubj	expression,lost
R7275	T26112	T26109	prep	in,lost
R7276	T26113	T26114	det	the,duct
R7277	T26114	T26112	pobj	duct,in
R7278	T26115	T26114	amod	nephric,duct
R7279	T26116	T26109	cc	and,lost
R728	T2993	T2994	compound	pygopus,mutant
R7280	T26117	T26109	conj	reduced,lost
R7281	T26118	T26117	prep	in,reduced
R7282	T26119	T26120	det	the,bud
R7283	T26120	T26118	pobj	bud,in
R7284	T26121	T26120	amod	ureteric,bud
R7285	T26122	T26123	punct	(,arrow
R7286	T26123	T26109	parataxis	arrow,lost
R7287	T26124	T26123	amod	white,arrow
R7288	T26125	T26123	punct	),arrow
R7289	T26126	T26096	punct	.,embryo
R729	T2994	T2992	compound	mutant,phenotype
R7290	T26128	T26129	punct	(,D
R7291	T26129	T26130	meta	D,staining
R7292	T26131	T26129	punct	),D
R7293	T26132	T26130	compound	Control,staining
R7294	T26133	T26130	compound	background,staining
R7295	T26134	T26135	compound	X,gal
R7296	T26135	T26130	compound	gal,staining
R7297	T26136	T26135	punct	-,gal
R7298	T26137	T26130	prep	of,staining
R7299	T26138	T26139	det	a,tract
R73	T1023	T1022	prep	of,%
R730	T2995	T2996	mark	that,dedicated
R7300	T26139	T26137	pobj	tract,of
R7301	T26140	T26139	amod	urogenital,tract
R7302	T26141	T26139	prep	from,tract
R7303	T26142	T26143	det	an,embryo
R7304	T26143	T26141	pobj	embryo,from
R7305	T26144	T26143	compound	E13.5,embryo
R7306	T26145	T26143	prep	without,embryo
R7307	T26146	T26147	det	the,transgene
R7308	T26147	T26145	pobj	transgene,without
R7309	T26148	T26149	compound	BAT,gal
R731	T2996	T2986	ccomp	dedicated,indicate
R7310	T26149	T26147	compound	gal,transgene
R7311	T26150	T26149	punct	-,gal
R7312	T26151	T26139	punct	(,tract
R7313	T26152	T26139	appos	Non-Tg,tract
R7314	T26153	T26139	punct	),tract
R7315	T26154	T26139	punct	", ",tract
R7316	T26155	T26139	acl	showing,tract
R7317	T26156	T26155	dobj	absence,showing
R7318	T26157	T26156	prep	of,absence
R7319	T26158	T26159	amod	endogenous,activity
R732	T2997	T2998	det	this,gene
R7320	T26159	T26157	pobj	activity,of
R7321	T26160	T26161	compound	beta,galactosidase
R7322	T26161	T26159	compound	galactosidase,activity
R7323	T26162	T26161	punct	-,galactosidase
R7324	T26163	T26130	punct	.,staining
R7325	T26165	T26166	punct	(,E
R7326	T26166	T26167	meta	E,Pygo1
R7327	T26168	T26166	punct	),E
R7328	T26169	T26167	punct	+,Pygo1
R7329	T26170	T26167	punct	/,Pygo1
R733	T2998	T2996	nsubjpass	gene,dedicated
R7330	T26171	T26167	punct	-,Pygo1
R7331	T26172	T26167	punct	/,Pygo1
R7332	T26173	T26167	appos	Pygo2,Pygo1
R7333	T26174	T26173	punct	+,Pygo2
R7334	T26175	T26173	punct	/,Pygo2
R7335	T26176	T26173	punct	+,Pygo2
R7336	T26177	T26167	punct	", ",Pygo1
R7337	T26178	T26167	prep	with,Pygo1
R7338	T26179	T26180	compound	BAT,gal
R7339	T26180	T26182	compound	gal,reporter
R734	T2999	T2996	auxpass	is,dedicated
R7340	T26181	T26180	punct	-,gal
R7341	T26182	T26183	compound	reporter,activity
R7342	T26183	T26178	pobj	activity,with
R7343	T26184	T26167	prep	in,Pygo1
R7344	T26185	T26186	det	the,compartment
R7345	T26186	T26184	pobj	compartment,in
R7346	T26187	T26186	amod	ureteric,compartment
R7347	T26188	T26186	prep	of,compartment
R7348	T26189	T26190	det	the,kidney
R7349	T26190	T26188	pobj	kidney,of
R735	T3000	T2996	prep	to,dedicated
R7350	T26191	T26190	amod	developing,kidney
R7351	T26192	T26186	punct	", ",compartment
R7352	T26193	T26186	prep	including,compartment
R7353	T26194	T26195	det	the,tips
R7354	T26195	T26193	pobj	tips,including
R7355	T26196	T26195	amod	ureteric,tips
R7356	T26197	T26195	punct	", ",tips
R7357	T26198	T26199	amod	ureteric,tree
R7358	T26199	T26195	conj	tree,tips
R7359	T26200	T26199	punct	", ",tree
R736	T3001	T2996	punct	", ",dedicated
R7360	T26201	T26199	cc	and,tree
R7361	T26202	T26199	conj	ureter,tree
R7362	T26203	T26167	punct	.,Pygo1
R7363	T26205	T26206	punct	(,F
R7364	T26206	T26207	meta	F,Pygo1
R7365	T26208	T26206	punct	),F
R7366	T26209	T26207	punct	+,Pygo1
R7367	T26210	T26207	punct	/,Pygo1
R7368	T26211	T26207	punct	-,Pygo1
R7369	T26212	T26207	punct	/,Pygo1
R737	T3002	T2996	cc	and,dedicated
R7370	T26213	T26207	appos	Pygo2,Pygo1
R7371	T26214	T26213	punct	-,Pygo2
R7372	T26215	T26213	punct	/,Pygo2
R7373	T26216	T26213	punct	-,Pygo2
R7374	T26217	T26207	punct	", ",Pygo1
R7375	T26218	T26207	prep	with,Pygo1
R7376	T26219	T26220	amod	marked,reduction
R7377	T26220	T26218	pobj	reduction,with
R7378	T26221	T26220	prep	of,reduction
R7379	T26222	T26223	compound	BAT,gal
R738	T3003	T2996	conj	required,dedicated
R7380	T26223	T26225	compound	gal,reporter
R7381	T26224	T26223	punct	-,gal
R7382	T26225	T26226	compound	reporter,activity
R7383	T26226	T26221	pobj	activity,of
R7384	T26227	T26220	prep	in,reduction
R7385	T26228	T26229	det	the,compartment
R7386	T26229	T26227	pobj	compartment,in
R7387	T26230	T26229	amod	ureteric,compartment
R7388	T26231	T26207	punct	.,Pygo1
R7389	T26233	T26234	punct	(,G
R739	T3004	T3003	prep	for,required
R7390	T26234	T26235	meta	G,Pygo1
R7391	T26236	T26234	punct	),G
R7392	T26237	T26235	punct	+,Pygo1
R7393	T26238	T26235	punct	/,Pygo1
R7394	T26239	T26235	punct	-,Pygo1
R7395	T26240	T26235	punct	/,Pygo1
R7396	T26241	T26235	appos	Pygo2,Pygo1
R7397	T26242	T26241	punct	+,Pygo2
R7398	T26243	T26241	punct	/,Pygo2
R7399	T26244	T26241	punct	-,Pygo2
R74	T1024	T1025	det	the,sequence
R740	T3005	T3003	punct	", ",required
R7400	T26245	T26235	punct	", ",Pygo1
R7401	T26246	T26235	prep	with,Pygo1
R7402	T26247	T26248	compound	reporter,expression
R7403	T26248	T26246	pobj	expression,with
R7404	T26249	T26248	prep	in,expression
R7405	T26250	T26251	det	the,duct
R7406	T26251	T26249	pobj	duct,in
R7407	T26252	T26251	amod	paramesonephric,duct
R7408	T26253	T26254	punct	(,arrowhead
R7409	T26254	T26251	parataxis	arrowhead,duct
R741	T3006	T3007	amod	canonical,signaling
R7410	T26255	T26254	amod	white,arrowhead
R7411	T26256	T26254	punct	),arrowhead
R7412	T26257	T26251	cc	and,duct
R7413	T26258	T26259	amod	ureteric,tree
R7414	T26259	T26251	conj	tree,duct
R7415	T26260	T26235	punct	.,Pygo1
R7416	T26262	T26263	punct	(,H
R7417	T26263	T26264	meta	H,Pygo1
R7418	T26265	T26263	punct	", ",H
R7419	T26266	T26263	dep	I,H
R742	T3007	T3003	conj	signaling,required
R7420	T26267	T26263	punct	),H
R7421	T26268	T26264	punct	-,Pygo1
R7422	T26269	T26264	punct	/,Pygo1
R7423	T26270	T26264	punct	-,Pygo1
R7424	T26271	T26264	punct	/,Pygo1
R7425	T26272	T26264	appos	Pygo2,Pygo1
R7426	T26273	T26272	punct	+,Pygo2
R7427	T26274	T26272	punct	/,Pygo2
R7428	T26275	T26272	punct	-,Pygo2
R7429	T26276	T26264	punct	", ",Pygo1
R743	T3008	T3007	compound	Wnt,signaling
R7430	T26277	T26264	prep	with,Pygo1
R7431	T26278	T26279	punct	(,H
R7432	T26279	T26280	meta	H,view
R7433	T26280	T26277	pobj	view,with
R7434	T26281	T26279	punct	),H
R7435	T26282	T26280	amod	ventral,view
R7436	T26283	T26280	acl	showing,view
R7437	T26284	T26285	compound	reporter,expression
R7438	T26285	T26283	dobj	expression,showing
R7439	T26286	T26283	prep	in,showing
R744	T3009	T2996	prep	during,dedicated
R7440	T26287	T26288	det	the,duct
R7441	T26288	T26286	pobj	duct,in
R7442	T26289	T26288	amod	paramesonephric,duct
R7443	T26290	T26291	punct	(,arrowhead
R7444	T26291	T26283	parataxis	arrowhead,showing
R7445	T26292	T26291	amod	white,arrowhead
R7446	T26293	T26291	punct	),arrowhead
R7447	T26294	T26280	punct	", ",view
R7448	T26295	T26280	cc	and,view
R7449	T26296	T26297	punct	(,I
R745	T3010	T3011	compound	Drosophila,development
R7450	T26297	T26298	meta	I,view
R7451	T26298	T26280	conj	view,view
R7452	T26299	T26297	punct	),I
R7453	T26300	T26298	amod	dorsal,view
R7454	T26301	T26298	acl	showing,view
R7455	T26302	T26303	amod	ureteric,tree
R7456	T26303	T26304	compound	tree,expression
R7457	T26304	T26301	dobj	expression,showing
R7458	T26305	T26301	prep	in,showing
R7459	T26306	T26307	det	the,kidney
R746	T3011	T3009	pobj	development,during
R7460	T26307	T26305	pobj	kidney,in
R7461	T26308	T26264	punct	.,Pygo1
R7462	T26310	T26311	punct	(,J
R7463	T26311	T26312	meta	J,Pygo1
R7464	T26313	T26311	punct	),J
R7465	T26314	T26312	punct	+,Pygo1
R7466	T26315	T26312	punct	/,Pygo1
R7467	T26316	T26312	punct	-,Pygo1
R7468	T26317	T26312	punct	/,Pygo1
R7469	T26318	T26312	appos	Pygo2,Pygo1
R747	T3012	T3013	punct	[,9
R7470	T26319	T26318	punct	+,Pygo2
R7471	T26320	T26318	punct	/,Pygo2
R7472	T26321	T26318	punct	-,Pygo2
R7473	T26322	T26312	punct	", ",Pygo1
R7474	T26323	T26324	det	a,control
R7475	T26324	T26312	appos	control,Pygo1
R7476	T26325	T26324	acl	processed,control
R7477	T26326	T26325	prep	in,processed
R7478	T26327	T26326	pobj	parallel,in
R7479	T26328	T26325	prep	with,processed
R748	T3013	T2986	parataxis	9,indicate
R7480	T26329	T26328	punct	(,with
R7481	T26330	T26328	pobj	K,with
R7482	T26331	T26330	punct	),K
R7483	T26332	T26330	cc	and,K
R7484	T26333	T26334	punct	(,L
R7485	T26334	T26330	conj	L,K
R7486	T26335	T26312	punct	),Pygo1
R7487	T26336	T26312	punct	", ",Pygo1
R7488	T26337	T26312	prep	with,Pygo1
R7489	T26338	T26337	pobj	expression,with
R749	T3014	T3013	punct	],9
R7490	T26339	T26338	prep	in,expression
R7491	T26340	T26341	amod	ureteric,tree
R7492	T26341	T26339	pobj	tree,in
R7493	T26342	T26341	cc	and,tree
R7494	T26343	T26344	amod	paramesonephric,duct
R7495	T26344	T26341	conj	duct,tree
R7496	T26345	T26312	punct	.,Pygo1
R7497	T26347	T26348	punct	(,K
R7498	T26348	T26349	meta	K,lost
R7499	T26350	T26348	punct	", ",K
R75	T1025	T1023	pobj	sequence,of
R750	T3015	T2986	punct	.,indicate
R7500	T26351	T26348	dep	L,K
R7501	T26352	T26348	punct	),K
R7502	T26353	T26354	nmod	Pygo1,reporter
R7503	T26354	T26364	compound	reporter,activity
R7504	T26355	T26353	punct	-,Pygo1
R7505	T26356	T26353	punct	/,Pygo1
R7506	T26357	T26353	punct	-,Pygo1
R7507	T26358	T26353	punct	/,Pygo1
R7508	T26359	T26353	appos	Pygo2,Pygo1
R7509	T26360	T26359	punct	-,Pygo2
R751	T3017	T3018	det	The,genome
R7510	T26361	T26359	punct	/,Pygo2
R7511	T26362	T26359	punct	-,Pygo2
R7512	T26363	T26354	punct	", ",reporter
R7513	T26364	T26349	nsubjpass	activity,lost
R7514	T26365	T26349	auxpass	was,lost
R7515	T26366	T26349	prep	in,lost
R7516	T26367	T26368	det	the,duct
R7517	T26368	T26366	pobj	duct,in
R7518	T26369	T26368	amod	paramesonephric,duct
R7519	T26370	T26371	punct	(,arrowhead
R752	T3018	T3020	nsubj	genome,carries
R7520	T26371	T26368	parataxis	arrowhead,duct
R7521	T26372	T26371	amod	white,arrowhead
R7522	T26373	T26371	punct	),arrowhead
R7523	T26374	T26366	punct	", ",in
R7524	T26375	T26366	cc	and,in
R7525	T26376	T26366	conj	in,in
R7526	T26377	T26378	det	the,ureter
R7527	T26378	T26376	pobj	ureter,in
R7528	T26379	T26378	cc	and,ureter
R7529	T26380	T26381	amod	ureteric,compartment
R753	T3019	T3018	amod	mammalian,genome
R7530	T26381	T26378	conj	compartment,ureter
R7531	T26382	T26381	prep	of,compartment
R7532	T26383	T26384	det	the,kidneys
R7533	T26384	T26382	pobj	kidneys,of
R7534	T26385	T26384	amod	developing,kidneys
R7535	T26386	T26387	punct	(,circles
R7536	T26387	T26378	parataxis	circles,ureter
R7537	T26388	T26387	amod	dashed,circles
R7538	T26389	T26387	punct	),circles
R7539	T26390	T26349	punct	", ",lost
R754	T3021	T3022	nummod	two,orthologs
R7540	T26391	T26349	prep	except,lost
R7541	T26392	T26391	prep	for,except
R7542	T26393	T26394	punct	(,K
R7543	T26394	T26395	parataxis	K,cells
R7544	T26395	T26392	pobj	cells,for
R7545	T26396	T26394	punct	),K
R7546	T26397	T26395	det	a,cells
R7547	T26398	T26395	amod	few,cells
R7548	T26399	T26400	advmod	faintly,staining
R7549	T26400	T26395	amod	staining,cells
R755	T3022	T3020	dobj	orthologs,carries
R7550	T26401	T26349	punct	.,lost
R7551	T26403	T26404	punct	(,M
R7552	T26404	T26405	meta	M,kidneys
R7553	T26406	T26404	punct	", ",M
R7554	T26407	T26404	dep	N,M
R7555	T26408	T26404	punct	),M
R7556	T26409	T26405	nmod	Pygo1,kidneys
R7557	T26410	T26409	punct	+,Pygo1
R7558	T26411	T26409	punct	/,Pygo1
R7559	T26412	T26409	punct	-,Pygo1
R756	T3023	T3021	punct	", ",two
R7560	T26413	T26409	punct	/,Pygo1
R7561	T26414	T26409	appos	Pygo2,Pygo1
R7562	T26415	T26414	punct	+,Pygo2
R7563	T26416	T26414	punct	/,Pygo2
R7564	T26417	T26414	punct	-,Pygo2
R7565	T26418	T26419	punct	(,left
R7566	T26419	T26409	parataxis	left,Pygo1
R7567	T26420	T26419	punct	),left
R7568	T26421	T26409	cc	and,Pygo1
R7569	T26422	T26409	conj	Pygo1,Pygo1
R757	T3024	T3021	cc	and,two
R7570	T26423	T26422	punct	-,Pygo1
R7571	T26424	T26422	punct	/,Pygo1
R7572	T26425	T26422	punct	-,Pygo1
R7573	T26426	T26422	punct	/,Pygo1
R7574	T26427	T26422	appos	Pygo2,Pygo1
R7575	T26428	T26427	punct	-,Pygo2
R7576	T26429	T26427	punct	/,Pygo2
R7577	T26430	T26427	punct	-,Pygo2
R7578	T26431	T26432	punct	(,right
R7579	T26432	T26422	parataxis	right,Pygo1
R758	T3025	T3026	advmod	only,two
R7580	T26433	T26432	punct	),right
R7581	T26434	T26405	compound	E18.5,kidneys
R7582	T26435	T26405	punct	.,kidneys
R7583	T26437	T26438	det	The,kidneys
R7584	T26438	T26439	nsubjpass	kidneys,bisected
R7585	T26440	T26438	prep	in,kidneys
R7586	T26441	T26440	punct	(,in
R7587	T26442	T26440	pobj	N,in
R7588	T26443	T26439	punct	),bisected
R7589	T26444	T26439	auxpass	were,bisected
R759	T3026	T3021	conj	two,two
R7590	T26445	T26439	punct	.,bisected
R7591	T26447	T26448	compound	BAT,gal
R7592	T26448	T26450	compound	gal,reporter
R7593	T26449	T26448	punct	-,gal
R7594	T26450	T26451	compound	reporter,expression
R7595	T26451	T26452	nsubjpass	expression,seen
R7596	T26453	T26452	auxpass	was,seen
R7597	T26454	T26452	prep	in,seen
R7598	T26455	T26456	det	the,components
R7599	T26456	T26454	pobj	components,in
R76	T1026	T1025	compound	coding,sequence
R760	T3027	T3022	punct	", ",orthologs
R7600	T26457	T26458	amod	ureteric,tree
R7601	T26458	T26456	compound	tree,components
R7602	T26459	T26456	prep	of,components
R7603	T26460	T26461	det	the,cortex
R7604	T26461	T26459	pobj	cortex,of
R7605	T26462	T26461	cc	and,cortex
R7606	T26463	T26461	conj	medulla,cortex
R7607	T26464	T26461	prep	of,cortex
R7608	T26465	T26466	det	the,heterozygotes
R7609	T26466	T26464	pobj	heterozygotes,of
R761	T3028	T3022	prep	of,orthologs
R7610	T26467	T26466	amod	double,heterozygotes
R7611	T26468	T26452	cc	but,seen
R7612	T26469	T26470	auxpass	was,lost
R7613	T26470	T26452	conj	lost,seen
R7614	T26471	T26472	advmod	almost,completely
R7615	T26472	T26470	advmod	completely,lost
R7616	T26473	T26470	prep	in,lost
R7617	T26474	T26475	det	the,mutants
R7618	T26475	T26473	pobj	mutants,in
R7619	T26476	T26477	amod	double,homozygous
R762	T3029	T3030	compound	Drosophila,Pygo
R7620	T26477	T26475	amod	homozygous,mutants
R7621	T26478	T26477	punct	-,homozygous
R7622	T26479	T26452	punct	.,seen
R7623	T26481	T26482	punct	(,O
R7624	T26482	T26483	meta	O,kidneys
R7625	T26484	T26482	punct	),O
R7626	T26485	T26483	nmod	Pygo1,kidneys
R7627	T26486	T26485	punct	+,Pygo1
R7628	T26487	T26485	punct	/,Pygo1
R7629	T26488	T26485	punct	-,Pygo1
R763	T3030	T3028	pobj	Pygo,of
R7630	T26489	T26485	punct	/,Pygo1
R7631	T26490	T26485	appos	Pygo2,Pygo1
R7632	T26491	T26490	punct	+,Pygo2
R7633	T26492	T26490	punct	/,Pygo2
R7634	T26493	T26490	punct	+,Pygo2
R7635	T26494	T26495	punct	(,left
R7636	T26495	T26485	parataxis	left,Pygo1
R7637	T26496	T26495	punct	),left
R7638	T26497	T26485	punct	", ",Pygo1
R7639	T26498	T26485	conj	Pygo1,Pygo1
R764	T3031	T3022	punct	", ",orthologs
R7640	T26499	T26498	punct	-,Pygo1
R7641	T26500	T26498	punct	/,Pygo1
R7642	T26501	T26498	punct	-,Pygo1
R7643	T26502	T26498	punct	/,Pygo1
R7644	T26503	T26498	appos	Pygo2,Pygo1
R7645	T26504	T26503	punct	+,Pygo2
R7646	T26505	T26503	punct	/,Pygo2
R7647	T26506	T26503	punct	-,Pygo2
R7648	T26507	T26508	punct	(,middle
R7649	T26508	T26498	parataxis	middle,Pygo1
R765	T3032	T3022	appos	Pygo1,orthologs
R7650	T26509	T26508	punct	),middle
R7651	T26510	T26498	punct	", ",Pygo1
R7652	T26511	T26498	cc	and,Pygo1
R7653	T26512	T26498	conj	Pygo1,Pygo1
R7654	T26513	T26512	punct	-,Pygo1
R7655	T26514	T26512	punct	/,Pygo1
R7656	T26515	T26512	punct	-,Pygo1
R7657	T26516	T26512	punct	/,Pygo1
R7658	T26517	T26512	appos	Pygo2,Pygo1
R7659	T26518	T26517	punct	-,Pygo2
R766	T3033	T3032	cc	and,Pygo1
R7660	T26519	T26517	punct	/,Pygo2
R7661	T26520	T26517	punct	-,Pygo2
R7662	T26521	T26522	punct	(,right
R7663	T26522	T26512	parataxis	right,Pygo1
R7664	T26523	T26522	punct	),right
R7665	T26524	T26483	compound	E18.5,kidneys
R7666	T26525	T26483	punct	.,kidneys
R7667	T26527	T26528	compound	Reporter,activity
R7668	T26528	T26529	nsubj	activity,was
R7669	T26530	T26529	acomp	present,was
R767	T3140	T3139	compound	branching,morphogenesis
R7670	T26531	T26529	prep	in,was
R7671	T26532	T26533	det	the,compartments
R7672	T26533	T26531	pobj	compartments,in
R7673	T26534	T26533	amod	ureteric,compartments
R7674	T26535	T26533	prep	of,compartments
R7675	T26536	T26537	det	the,kidneys
R7676	T26537	T26535	pobj	kidneys,of
R7677	T26538	T26537	nmod	Pygo1,kidneys
R7678	T26539	T26538	punct	+,Pygo1
R7679	T26540	T26538	punct	/,Pygo1
R768	T3141	T3139	prep	of,morphogenesis
R7680	T26541	T26538	punct	-,Pygo1
R7681	T26542	T26538	punct	/,Pygo1
R7682	T26543	T26538	appos	Pygo2,Pygo1
R7683	T26544	T26543	punct	+,Pygo2
R7684	T26545	T26543	punct	/,Pygo2
R7685	T26546	T26543	punct	+,Pygo2
R7686	T26547	T26548	punct	(,left
R7687	T26548	T26538	parataxis	left,Pygo1
R7688	T26549	T26548	punct	),left
R7689	T26550	T26538	cc	and,Pygo1
R769	T3142	T3143	det	the,bud
R7690	T26551	T26538	conj	Pygo1,Pygo1
R7691	T26552	T26551	punct	-,Pygo1
R7692	T26553	T26551	punct	/,Pygo1
R7693	T26554	T26551	punct	-,Pygo1
R7694	T26555	T26551	punct	/,Pygo1
R7695	T26556	T26551	appos	Pygo2,Pygo1
R7696	T26557	T26556	punct	+,Pygo2
R7697	T26558	T26556	punct	/,Pygo2
R7698	T26559	T26556	punct	-,Pygo2
R7699	T26560	T26561	punct	(,middle
R77	T1027	T1022	punct	", ",%
R770	T3143	T3141	pobj	bud,of
R7700	T26561	T26551	parataxis	middle,Pygo1
R7701	T26562	T26561	punct	),middle
R7702	T26563	T26529	punct	", ",was
R7703	T26564	T26529	cc	but,was
R7704	T26565	T26529	conj	lost,was
R7705	T26566	T26565	prep	in,lost
R7706	T26567	T26568	det	the,kidney
R7707	T26568	T26566	pobj	kidney,in
R7708	T26569	T26568	nmod	Pygo1,kidney
R7709	T26570	T26569	punct	-,Pygo1
R771	T3144	T3143	amod	ureteric,bud
R7710	T26571	T26569	punct	/,Pygo1
R7711	T26572	T26569	punct	-,Pygo1
R7712	T26573	T26569	punct	/,Pygo1
R7713	T26574	T26569	appos	Pygo2,Pygo1
R7714	T26575	T26574	punct	-,Pygo2
R7715	T26576	T26574	punct	/,Pygo2
R7716	T26577	T26574	punct	-,Pygo2
R7717	T26578	T26579	punct	(,right
R7718	T26579	T26569	parataxis	right,Pygo1
R7719	T26580	T26579	punct	),right
R772	T3145	T3139	punct	", ",morphogenesis
R7720	T26581	T26529	punct	.,was
R7721	T26583	T26584	amod	Original,magnification
R7722	T26584	T26585	dep	magnification,32
R7723	T26586	T26585	punct	: ,32
R7724	T26587	T26588	punct	(,A
R7725	T26588	T26585	parataxis	A,32
R7726	T26589	T26590	punct	-,C
R7727	T26590	T26588	prep	C,A
R7728	T26591	T26588	punct	),A
R7729	T26592	T26585	punct	×,32
R773	T3146	T3139	cc	and,morphogenesis
R7730	T26593	T26585	punct	", ",32
R7731	T26594	T26595	punct	(,D
R7732	T26595	T26596	parataxis	D,63
R7733	T26596	T26585	conj	63,32
R7734	T26597	T26598	punct	-,F
R7735	T26598	T26595	prep	F,D
R7736	T26599	T26595	punct	),D
R7737	T26600	T26596	punct	×,63
R7738	T26601	T26596	punct	", ",63
R7739	T26602	T26603	punct	(,G
R774	T3147	T3139	conj	expansion,morphogenesis
R7740	T26603	T26604	parataxis	G,40
R7741	T26604	T26596	conj	40,63
R7742	T26605	T26606	punct	-,I
R7743	T26606	T26603	prep	I,G
R7744	T26607	T26603	punct	),G
R7745	T26608	T26604	punct	×,40
R7746	T26609	T26604	punct	", ",40
R7747	T26610	T26611	punct	(,J
R7748	T26611	T26612	parataxis	J,50
R7749	T26612	T26604	conj	50,40
R775	T3148	T3147	prep	of,expansion
R7750	T26613	T26614	punct	-,L
R7751	T26614	T26611	prep	L,J
R7752	T26615	T26611	punct	),J
R7753	T26616	T26612	punct	×,50
R7754	T26617	T26612	punct	", ",50
R7755	T26618	T26619	punct	(,N
R7756	T26619	T26622	parataxis	N,10
R7757	T26620	T26619	nmod	M,N
R7758	T26621	T26619	punct	", ",N
R7759	T26622	T26612	conj	10,50
R776	T3149	T3150	det	the,zone
R7760	T26623	T26619	punct	),N
R7761	T26624	T26622	punct	×,10
R7762	T26625	T26622	punct	", ",10
R7763	T26626	T26622	cc	and,10
R7764	T26627	T26628	punct	(,O
R7765	T26628	T26629	parataxis	O,12.5
R7766	T26629	T26622	conj	12.5,10
R7767	T26630	T26628	punct	),O
R7768	T26631	T26629	punct	×,12.5
R7769	T26632	T26585	punct	.,32
R777	T3150	T3148	pobj	zone,of
R7770	T26917	T26918	amod	Quantitative,analysis
R7771	T26919	T26918	prep	of,analysis
R7772	T26920	T26921	compound	BAT,gal
R7773	T26921	T26923	compound	gal,reporter
R7774	T26922	T26921	punct	-,gal
R7775	T26923	T26924	compound	reporter,expression
R7776	T26924	T26919	pobj	expression,of
R7777	T26925	T26924	prep	in,expression
R7778	T26926	T26927	compound	Pygo1,Pygo2
R7779	T26927	T26929	compound	Pygo2,extracts
R778	T3151	T3150	prep	of,zone
R7780	T26928	T26927	punct	/,Pygo2
R7781	T26929	T26925	pobj	extracts,in
R7782	T26930	T26929	compound	E18.5,extracts
R7783	T26931	T26929	compound	kidney,extracts
R7784	T26932	T26918	punct	.,analysis
R7785	T26934	T26935	npadvmod	Transgene,specific
R7786	T26935	T26937	amod	specific,galactosidase
R7787	T26936	T26935	punct	-,specific
R7788	T26937	T26940	nsubjpass	galactosidase,quantified
R7789	T26938	T26937	compound	beta,galactosidase
R779	T3152	T3153	amod	condensed,mesenchyme
R7790	T26939	T26937	punct	-,galactosidase
R7791	T26941	T26940	auxpass	was,quantified
R7792	T26942	T26940	prep	by,quantified
R7793	T26943	T26944	compound	ELISA,analysis
R7794	T26944	T26942	pobj	analysis,by
R7795	T26945	T26940	punct	.,quantified
R7796	T26947	T26948	nmod	Pygo1,kidneys
R7797	T26948	T26977	nsubj	kidneys,showed
R7798	T26949	T26947	punct	+,Pygo1
R7799	T26950	T26947	punct	/,Pygo1
R78	T1028	T1022	prep	including,%
R780	T3153	T3151	pobj	mesenchyme,of
R7800	T26951	T26947	punct	-,Pygo1
R7801	T26952	T26947	punct	/,Pygo1
R7802	T26953	T26947	appos	Pygo2,Pygo1
R7803	T26954	T26953	punct	+,Pygo2
R7804	T26955	T26953	punct	/,Pygo2
R7805	T26956	T26953	punct	+,Pygo2
R7806	T26957	T26947	punct	", ",Pygo1
R7807	T26958	T26947	conj	Pygo1,Pygo1
R7808	T26959	T26958	punct	+,Pygo1
R7809	T26960	T26958	punct	/,Pygo1
R781	T3034	T3032	conj	Pygo2,Pygo1
R7810	T26961	T26958	punct	-,Pygo1
R7811	T26962	T26958	punct	/,Pygo1
R7812	T26963	T26958	appos	Pygo2,Pygo1
R7813	T26964	T26963	punct	+,Pygo2
R7814	T26965	T26963	punct	/,Pygo2
R7815	T26966	T26963	punct	-,Pygo2
R7816	T26967	T26958	cc	and,Pygo1
R7817	T26968	T26958	conj	Pygo1,Pygo1
R7818	T26969	T26968	punct	+,Pygo1
R7819	T26970	T26968	punct	/,Pygo1
R782	T3154	T3150	acl	surrounding,zone
R7820	T26971	T26968	punct	-,Pygo1
R7821	T26972	T26968	punct	/,Pygo1
R7822	T26973	T26968	appos	Pygo2,Pygo1
R7823	T26974	T26973	punct	+,Pygo2
R7824	T26975	T26973	punct	/,Pygo2
R7825	T26976	T26973	punct	+,Pygo2
R7826	T26978	T26948	appos	all,kidneys
R7827	T26979	T26980	amod	similar,levels
R7828	T26980	T26977	dobj	levels,showed
R7829	T26981	T26980	prep	of,levels
R783	T3035	T3036	punct	[,14
R7830	T26982	T26983	compound	BAT,gal
R7831	T26983	T26985	compound	gal,expression
R7832	T26984	T26983	punct	-,gal
R7833	T26985	T26981	pobj	expression,of
R7834	T26986	T26977	punct	.,showed
R7835	T26988	T26989	prep	In,reduced
R7836	T26990	T26991	det	the,mutants
R7837	T26991	T26988	pobj	mutants,In
R7838	T26992	T26993	nmod	Pygo2,hetereozygote
R7839	T26993	T26991	nmod	hetereozygote,mutants
R784	T3155	T3156	det	the,bud
R7840	T26994	T26991	punct	", ",mutants
R7841	T26995	T26996	npadvmod	Pygo1,homozygous
R7842	T26996	T26991	amod	homozygous,mutants
R7843	T26997	T26989	punct	", ",reduced
R7844	T26998	T26989	advmod	however,reduced
R7845	T26999	T26989	punct	", ",reduced
R7846	T27000	T27001	compound	reporter,expression
R7847	T27001	T26989	nsubjpass	expression,reduced
R7848	T27002	T26989	auxpass	was,reduced
R7849	T27003	T26989	prep	by,reduced
R785	T3036	T3020	parataxis	14,carries
R7850	T27004	T27005	quantmod	about,50
R7851	T27005	T27006	nummod	50,%
R7852	T27006	T27003	pobj	%,by
R7853	T27007	T26989	punct	.,reduced
R7854	T27009	T27010	prep	In,was
R7855	T27011	T27012	npadvmod	Pygo2,homozygous
R7856	T27012	T27013	amod	homozygous,mutants
R7857	T27013	T27009	pobj	mutants,In
R7858	T27014	T27015	compound	BAT,gal
R7859	T27015	T27017	compound	gal,expression
R786	T3037	T3036	nummod	8,14
R7860	T27016	T27015	punct	-,gal
R7861	T27017	T27010	nsubj	expression,was
R7862	T27018	T27019	advmod	uniformly,low
R7863	T27019	T27010	acomp	low,was
R7864	T27020	T27010	punct	", ",was
R7865	T27021	T27010	prep	with,was
R7866	T27022	T27021	cc	or,with
R7867	T27023	T27021	conj	without,with
R7868	T27024	T27025	amod	wild,type
R7869	T27025	T27027	compound	type,alleles
R787	T3156	T3154	dobj	bud,surrounding
R7870	T27026	T27025	punct	-,type
R7871	T27027	T27023	pobj	alleles,without
R7872	T27028	T27027	compound	Pygo1,alleles
R7873	T27029	T27010	punct	.,was
R7874	T27031	T27032	det	Each,group
R7875	T27032	T27034	nsubj	group,included
R7876	T27033	T27032	amod	experimental,group
R7877	T27035	T27036	det	a,size
R7878	T27036	T27034	dobj	size,included
R7879	T27037	T27036	compound	sample,size
R788	T3038	T3036	punct	",",14
R7880	T27038	T27036	prep	of,size
R7881	T27039	T27040	advmod	at,four
R7882	T27040	T27038	pobj	four,of
R7883	T27041	T27040	advmod	least,four
R7884	T27042	T27034	punct	.,included
R7885	T27272	T27273	compound	Gene,expression
R7886	T27273	T27274	compound	expression,changes
R7887	T27275	T27274	prep	of,changes
R7888	T27276	T27277	amod	common,targets
R7889	T27277	T27275	pobj	targets,of
R789	T3039	T3036	nummod	9,14
R7890	T27278	T27277	compound	Wnt,targets
R7891	T27279	T27277	compound	signaling,targets
R7892	T27280	T27274	prep	in,changes
R7893	T27281	T27282	det	the,kidney
R7894	T27282	T27280	pobj	kidney,in
R7895	T27283	T27282	nmod	E18.5,kidney
R7896	T27284	T27285	compound	Pygo1,Pygo2
R7897	T27285	T27287	npadvmod	Pygo2,null
R7898	T27286	T27285	punct	/,Pygo2
R7899	T27287	T27282	amod	null,kidney
R79	T1029	T1030	det	the,domain
R790	T3040	T3036	punct	",",14
R7900	T27288	T27274	punct	.,changes
R7901	T27290	T27291	compound	Microarray,analysis
R7902	T27291	T27292	nsubjpass	analysis,performed
R7903	T27293	T27292	auxpass	was,performed
R7904	T27294	T27292	prep	in,performed
R7905	T27295	T27294	pobj	triplicate,in
R7906	T27296	T27292	prep	on,performed
R7907	T27297	T27298	amod	wild,type
R7908	T27298	T27300	nmod	type,kidneys
R7909	T27299	T27298	punct	-,type
R791	T3157	T3156	amod	ureteric,bud
R7910	T27300	T27296	pobj	kidneys,on
R7911	T27301	T27298	cc	and,type
R7912	T27302	T27303	npadvmod	Pygo1,null
R7913	T27303	T27298	conj	null,type
R7914	T27304	T27302	punct	/,Pygo1
R7915	T27305	T27302	nummod	2,Pygo1
R7916	T27306	T27303	npadvmod	compound,null
R7917	T27307	T27300	compound	E18.5,kidneys
R7918	T27308	T27292	punct	.,performed
R7919	T27310	T27311	amod	Possible,targets
R792	T3041	T3036	punct	],14
R7920	T27311	T27313	nsubjpass	targets,selected
R7921	T27312	T27311	compound	Wnt,targets
R7922	T27314	T27313	auxpass	were,selected
R7923	T27315	T27313	prep	from,selected
R7924	T27316	T27315	pobj	those,from
R7925	T27317	T27316	acl	compiled,those
R7926	T27318	T27317	prep	at,compiled
R7927	T27319	T27318	punct	[,at
R7928	T27320	T27318	pobj	39,at
R7929	T27321	T27313	punct	],selected
R793	T3042	T3020	punct	.,carries
R7930	T27322	T27313	punct	.,selected
R7931	T27324	T27325	det	An,list
R7932	T27325	T27328	nsubjpass	list,created
R7933	T27326	T27325	amod	initial,list
R7934	T27327	T27325	compound	gene,list
R7935	T27329	T27325	prep	of,list
R7936	T27330	T27331	nummod	82,targets
R7937	T27331	T27329	pobj	targets,of
R7938	T27332	T27331	compound	Wnt,targets
R7939	T27333	T27328	auxpass	was,created
R794	T3158	T3139	punct	", ",morphogenesis
R7940	T27334	T27328	cc	and,created
R7941	T27335	T27336	advmod	then,reduced
R7942	T27336	T27328	conj	reduced,created
R7943	T27337	T27336	prep	to,reduced
R7944	T27338	T27339	det	a,total
R7945	T27339	T27337	pobj	total,to
R7946	T27340	T27339	prep	of,total
R7947	T27341	T27342	nummod	40,genes
R7948	T27342	T27340	pobj	genes,of
R7949	T27343	T27336	advcl	using,reduced
R795	T3044	T3045	prep	In,generated
R7950	T27344	T27345	det	an,restriction
R7951	T27345	T27343	dobj	restriction,using
R7952	T27346	T27347	compound	expression,level
R7953	T27347	T27345	compound	level,restriction
R7954	T27348	T27345	acl	requiring,restriction
R7955	T27349	T27350	det	the,intensity
R7956	T27350	T27348	dobj	intensity,requiring
R7957	T27351	T27350	amod	raw,intensity
R7958	T27352	T27350	compound	expression,intensity
R7959	T27353	T27354	aux	to,be
R796	T3159	T3139	cc	yet,morphogenesis
R7960	T27354	T27348	xcomp	be,requiring
R7961	T27355	T27356	punct	>,100
R7962	T27356	T27354	attr	100,be
R7963	T27357	T27354	prep	in,be
R7964	T27358	T27359	advmod	at,3
R7965	T27359	T27361	nummod	3,samples
R7966	T27360	T27359	advmod	least,3
R7967	T27361	T27357	pobj	samples,in
R7968	T27362	T27328	punct	.,created
R7969	T27364	T27365	nmod	Pygo1,probes
R797	T3046	T3047	det	this,report
R7970	T27365	T27368	nsubjpass	probes,included
R7971	T27366	T27364	cc	and,Pygo1
R7972	T27367	T27364	conj	Pygo2,Pygo1
R7973	T27369	T27368	auxpass	were,included
R7974	T27370	T27371	aux	to,demonstrate
R7975	T27371	T27368	advcl	demonstrate,included
R7976	T27372	T27373	amod	significant,changes
R7977	T27373	T27371	dobj	changes,demonstrate
R7978	T27374	T27373	prep	in,changes
R7979	T27375	T27376	compound	expression,levels
R798	T3047	T3044	pobj	report,In
R7980	T27376	T27374	pobj	levels,in
R7981	T27377	T27368	punct	.,included
R7982	T27424	T27423	prep	of,Phenotypes
R7983	T27425	T27426	dep	Pygo2,type
R7984	T27426	T27429	nmod	type,embryos
R7985	T27427	T27426	amod	wild,type
R7986	T27428	T27426	punct	-,type
R7987	T27429	T27424	pobj	embryos,of
R7988	T27430	T27426	punct	", ",type
R7989	T27431	T27426	conj	heterozyogous,type
R799	T3160	T3161	advmod	relatively,normal
R7990	T27432	T27431	punct	", ",heterozyogous
R7991	T27433	T27431	cc	and,heterozyogous
R7992	T27434	T27431	conj	null,heterozyogous
R7993	T27435	T27429	compound	E18.5,embryos
R7994	T27436	T27429	prep	on,embryos
R7995	T27437	T27438	det	a,background
R7996	T27438	T27436	pobj	background,on
R7997	T27439	T27440	npadvmod	Pygo1,null
R7998	T27440	T27438	amod	null,background
R7999	T27441	T27423	punct	.,Phenotypes
R8	T947	T948	compound	Wnt,signaling
R80	T1030	T1028	pobj	domain,including
R800	T3048	T3045	punct	", ",generated
R8000	T27487	T27488	amod	Confocal,analysis
R8001	T27489	T27488	prep	of,analysis
R8002	T27490	T27491	dep	Pygo2,type
R8003	T27491	T27494	nmod	type,kidneys
R8004	T27492	T27491	amod	wild,type
R8005	T27493	T27491	punct	-,type
R8006	T27494	T27489	pobj	kidneys,of
R8007	T27495	T27491	punct	", ",type
R8008	T27496	T27491	conj	heterozygous,type
R8009	T27497	T27496	punct	", ",heterozygous
R801	T3049	T3045	nsubj	we,generated
R8010	T27498	T27496	cc	and,heterozygous
R8011	T27499	T27496	conj	null,heterozygous
R8012	T27500	T27494	compound	E18.5,kidneys
R8013	T27501	T27488	prep	on,analysis
R8014	T27502	T27503	det	the,background
R8015	T27503	T27501	pobj	background,on
R8016	T27504	T27505	npadvmod	Pygo1,null
R8017	T27505	T27503	amod	null,background
R8018	T27506	T27488	punct	.,analysis
R8019	T27573	T27574	advmod	differentially,expressed
R802	T3050	T3051	amod	targeted,mutations
R8020	T27574	T27572	acl	expressed,Genes
R8021	T27575	T27576	punct	(,change
R8022	T27576	T27574	parataxis	change,expressed
R8023	T27577	T27578	nsubj	p,0.05
R8024	T27578	T27576	ccomp	0.05,change
R8025	T27579	T27578	punct	<,0.05
R8026	T27580	T27576	punct	", ",change
R8027	T27581	T27576	amod	two-fold,change
R8028	T27582	T27583	cc	or,greater
R8029	T27583	T27576	nummod	greater,change
R803	T3051	T3045	dobj	mutations,generated
R8030	T27584	T27576	punct	),change
R8031	T27585	T27574	prep	in,expressed
R8032	T27586	T27587	det	the,kidney
R8033	T27587	T27585	pobj	kidney,in
R8034	T27588	T27589	compound	Pygo1,Pygo2
R8035	T27589	T27591	npadvmod	Pygo2,null
R8036	T27590	T27589	punct	/,Pygo2
R8037	T27591	T27587	amod	null,kidney
R8038	T27592	T27587	compound	E18.5,kidney
R8039	T27593	T27587	acl	normalized,kidney
R804	T3161	T3162	amod	normal,formation
R8040	T27594	T27593	prep	to,normalized
R8041	T27595	T27596	amod	wild,type
R8042	T27596	T27598	compound	type,samples
R8043	T27597	T27596	punct	-,type
R8044	T27598	T27594	pobj	samples,to
R8045	T27599	T27572	punct	.,Genes
R8046	T27656	T27655	prep	of,Validation
R8047	T27657	T27658	compound	gene,expression
R8048	T27658	T27659	compound	expression,changes
R8049	T27659	T27656	pobj	changes,of
R805	T3052	T3051	prep	of,mutations
R8050	T27660	T27659	prep	in,changes
R8051	T27661	T27662	det	the,kidneys
R8052	T27662	T27660	pobj	kidneys,in
R8053	T27663	T27662	nmod	E18.5,kidneys
R8054	T27664	T27665	compound	Pygo1,Pygo2
R8055	T27665	T27667	npadvmod	Pygo2,null
R8056	T27666	T27665	punct	/,Pygo2
R8057	T27667	T27662	amod	null,kidneys
R8058	T27668	T27659	acl	normalized,changes
R8059	T27669	T27668	prep	to,normalized
R806	T3053	T3052	pobj	Pygo1,of
R8060	T27670	T27671	nmod	E18.5,kidneys
R8061	T27671	T27669	pobj	kidneys,to
R8062	T27672	T27673	amod	wild,type
R8063	T27673	T27671	compound	type,kidneys
R8064	T27674	T27673	punct	-,type
R8065	T27675	T27655	punct	.,Validation
R807	T3054	T3053	cc	and,Pygo1
R808	T3162	T3139	conj	formation,morphogenesis
R809	T3055	T3053	conj	Pygo2,Pygo1
R81	T1031	T1030	amod	critical,domain
R810	T3056	T3057	aux	to,determine
R811	T3163	T3162	compound	nephron,formation
R812	T3057	T3045	advcl	determine,generated
R813	T3058	T3059	poss	their,functions
R814	T3059	T3057	dobj	functions,determine
R815	T3060	T3057	punct	", ",determine
R816	T3061	T3057	prep	with,determine
R817	T3062	T3063	det	a,interest
R818	T3164	T3137	punct	", ",showed
R819	T3063	T3061	pobj	interest,with
R82	T1032	T1030	compound	PHD,domain
R820	T3064	T3063	amod	particular,interest
R821	T3065	T3063	prep	in,interest
R822	T3165	T3166	mark	as,measured
R823	T3066	T3067	det	the,contributions
R824	T3067	T3065	pobj	contributions,in
R825	T3166	T3137	advcl	measured,showed
R826	T3068	T3067	prep	of,contributions
R827	T3069	T3070	det	these,genes
R828	T3167	T3166	prep	by,measured
R829	T3070	T3068	pobj	genes,of
R83	T1033	T1018	punct	", ",resulted
R830	T3071	T3067	prep	to,contributions
R831	T3072	T3073	amod	canonical,signaling
R832	T3168	T3167	pobj	histology,by
R833	T3073	T3071	pobj	signaling,to
R834	T3074	T3073	compound	Wnt,signaling
R835	T3075	T3073	prep	during,signaling
R836	T3169	T3168	punct	", ",histology
R837	T3076	T3077	compound	kidney,development
R838	T3077	T3075	pobj	development,during
R839	T3078	T3045	punct	.,generated
R84	T1034	T1018	cc	and,resulted
R840	T3170	T3171	amod	confocal,analysis
R841	T3080	T3081	det	The,embryos
R842	T3081	T3087	nsubj	embryos,showed
R843	T3171	T3168	conj	analysis,histology
R844	T3082	T3081	amod	resulting,embryos
R845	T3083	T3084	amod	double,homozygous
R846	T3084	T3081	amod	homozygous,embryos
R847	T3172	T3171	punct	", ",analysis
R848	T3085	T3084	punct	-,homozygous
R849	T3086	T3081	compound	mutant,embryos
R85	T1035	T1036	advmod	almost,certainly
R850	T3173	T3174	advmod	in,situ
R851	T3088	T3089	det	a,reduction
R852	T3089	T3087	dobj	reduction,showed
R853	T3174	T3175	amod	situ,hybridization
R854	T3090	T3091	npadvmod	context,dependent
R855	T3091	T3089	amod	dependent,reduction
R856	T3092	T3091	punct	-,dependent
R857	T3093	T3089	prep	in,reduction
R858	T3175	T3171	conj	hybridization,analysis
R859	T3094	T3095	amod	canonical,signaling
R86	T1036	T1018	advmod	certainly,resulted
R860	T3095	T3093	pobj	signaling,in
R861	T3176	T3175	cc	and,hybridization
R862	T3096	T3095	compound	Wnt,signaling
R863	T3097	T3098	mark	as,measured
R864	T3098	T3087	advcl	measured,showed
R865	T3177	T3178	compound	microarray,analysis
R866	T3099	T3098	agent	by,measured
R867	T3100	T3101	compound	Wnt,reporter
R868	T3178	T3175	conj	analysis,hybridization
R869	T3101	T3102	compound	reporter,transgene
R87	T1037	T1018	prep	in,resulted
R870	T3102	T3103	compound	transgene,expression
R871	T3103	T3099	pobj	expression,by
R872	T3179	T3131	punct	.,focused
R873	T3104	T3087	punct	.,showed
R874	T3181	T3182	det	The,conclusion
R875	T3106	T3107	nsubj	Development,remained
R876	T3108	T3107	punct	", ",remained
R877	T3182	T3184	nsubj	conclusion,is
R878	T3109	T3107	advmod	however,remained
R879	T3110	T3107	punct	", ",remained
R88	T1038	T1039	amod	null,function
R880	T3111	T3112	advmod	surprisingly,normal
R881	T3183	T3182	amod	obvious,conclusion
R882	T3112	T3107	acomp	normal,remained
R883	T3113	T3107	punct	", ",remained
R884	T3114	T3107	prep	with,remained
R885	T3185	T3186	mark	that,is
R886	T3115	T3114	pobj	survival,with
R887	T3116	T3115	prep	to,survival
R888	T3117	T3116	pobj	birth,to
R889	T3118	T3115	cc	and,survival
R89	T1039	T1037	pobj	function,in
R890	T3186	T3184	ccomp	is,is
R891	T3119	T3120	amod	few,defects
R892	T3120	T3115	conj	defects,survival
R893	T3121	T3120	amod	apparent,defects
R894	T3122	T3120	prep	in,defects
R895	T3123	T3124	amod	most,systems
R896	T3187	T3186	prep	in,is
R897	T3124	T3122	pobj	systems,in
R898	T3125	T3124	compound	organ,systems
R899	T3126	T3107	punct	.,remained
R9	T948	T946	pobj	signaling,in
R90	T1040	T1018	punct	.,resulted
R900	T3128	T3129	poss	Our,analysis
R901	T3188	T3187	pobj	mammals,in
R902	T3129	T3131	nsubj	analysis,focused
R903	T3130	T3129	amod	phenotypic,analysis
R904	T3189	T3188	punct	", ",mammals
R905	T3132	T3131	prep	on,focused
R906	T3133	T3134	det	the,kidney
R907	T3190	T3188	prep	unlike,mammals
R908	T3134	T3132	pobj	kidney,on
R909	T3135	T3134	punct	", ",kidney
R91	T1042	T1043	npadvmod	Pygo2,homozygous
R910	T3136	T3137	dep	which,showed
R911	T3137	T3134	relcl	showed,kidney
R912	T3191	T3190	pobj	Drosophila,unlike
R913	T3138	T3139	amod	altered,morphogenesis
R914	T3139	T3137	dobj	morphogenesis,showed
R915	T3192	T3186	punct	", ",is
R916	T3193	T3194	npadvmod	Pygopus,mediated
R917	T3194	T3196	amod	mediated,signaling
R918	T3195	T3194	punct	-,mediated
R919	T3196	T3186	nsubj	signaling,is
R92	T1043	T1044	amod	homozygous,mutants
R920	T3197	T3196	amod	canonical,signaling
R921	T3198	T3196	compound	Wnt,signaling
R922	T3199	T3186	neg	not,is
R923	T3200	T3201	advmod	absolutely,necessary
R924	T3201	T3186	acomp	necessary,is
R925	T3202	T3186	prep	in,is
R926	T3203	T3204	amod	most,systems
R927	T3204	T3202	pobj	systems,in
R928	T3205	T3204	amod	developing,systems
R929	T3206	T3204	compound	organ,systems
R93	T1044	T1045	nsubj	mutants,died
R930	T3207	T3184	punct	.,is
R931	T3498	T3499	npadvmod	Pygo1,targeted
R932	T3499	T3502	amod	targeted,mutations
R933	T3500	T3498	cc	and,Pygo1
R934	T3501	T3498	conj	Pygo2,Pygo1
R935	T3504	T3505	nsubj	We,targeted
R936	T3506	T3507	preconj	both,genes
R937	T3507	T3505	dobj	genes,targeted
R938	T3508	T3507	det	the,genes
R939	T3509	T3507	nmod	Pygo1,genes
R94	T1046	T1045	punct	", ",died
R940	T3510	T3509	cc	and,Pygo1
R941	T3511	T3509	conj	Pygo2,Pygo1
R942	T3512	T3505	prep	by,targeted
R943	T3513	T3512	pcomp	inserting,by
R944	T3514	T3515	compound	LoxP,sequences
R945	T3515	T3513	dobj	sequences,inserting
R946	T3516	T3517	aux	to,flank
R947	T3517	T3513	advcl	flank,inserting
R948	T3518	T3519	amod	critical,regions
R949	T3519	T3517	dobj	regions,flank
R95	T1047	T1045	prep	with,died
R950	T3520	T3519	amod	coding,regions
R951	T3521	T3519	prep	including,regions
R952	T3522	T3523	det	the,domains
R953	T3523	T3521	pobj	domains,including
R954	T3524	T3523	compound	PHD,domains
R955	T3525	T3505	punct	.,targeted
R956	T3527	T3528	det	The,mice
R957	T3528	T3531	nsubjpass	mice,mated
R958	T3529	T3528	amod	resulting,mice
R959	T3530	T3528	amod	targeted,mice
R96	T1048	T1049	amod	rare,exception
R960	T3532	T3531	auxpass	were,mated
R961	T3533	T3531	prep	with,mated
R962	T3534	T3535	amod	transgenic,mice
R963	T3535	T3533	pobj	mice,with
R964	T3536	T3537	compound	CMV,Cre
R965	T3537	T3535	compound	Cre,mice
R966	T3538	T3537	punct	-,Cre
R967	T3539	T3540	punct	[,15
R968	T3540	T3535	parataxis	15,mice
R969	T3541	T3540	punct	],15
R97	T1049	T1047	pobj	exception,with
R970	T3542	T3543	aux	to,drive
R971	T3543	T3531	advcl	drive,mated
R972	T3544	T3545	compound	germline,LoxP
R973	T3545	T3546	compound	LoxP,recombination
R974	T3546	T3543	dobj	recombination,drive
R975	T3547	T3531	punct	", ",mated
R976	T3548	T3531	advcl	resulting,mated
R977	T3549	T3548	prep	in,resulting
R978	T3550	T3551	det	the,alleles
R979	T3551	T3549	pobj	alleles,in
R98	T1050	T1045	punct	", ",died
R980	T3552	T3551	amod	null,alleles
R981	T3553	T3551	compound	mutant,alleles
R982	T3554	T3555	dep	that,used
R983	T3555	T3551	relcl	used,alleles
R984	T3556	T3555	auxpass	were,used
R985	T3557	T3555	prep	for,used
R986	T3558	T3559	det	this,study
R987	T3559	T3557	pobj	study,for
R988	T3560	T3531	punct	.,mated
R989	T3562	T3563	nsubj	PCR,confirmed
R99	T1051	T1052	advmod	shortly,after
R990	T3564	T3565	det	the,deletion
R991	T3565	T3563	dobj	deletion,confirmed
R992	T3566	T3565	prep	of,deletion
R993	T3567	T3568	det	the,bulk
R994	T3568	T3566	pobj	bulk,of
R995	T3569	T3568	prep	of,bulk
R996	T3570	T3571	det	the,sequences
R997	T3571	T3569	pobj	sequences,of
R998	T3572	T3571	amod	coding,sequences
R999	T3573	T3571	prep	for,sequences

PMC:1858683 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

PMC:1858683 JSON TXT 8 Projects