| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T25223 |
0-12 |
JJ |
denotes |
Histological |
| T25224 |
13-21 |
NN |
denotes |
analysis |
| T25225 |
22-25 |
CC |
denotes |
and |
| T25226 |
26-31 |
NN |
denotes |
TUNEL |
| T25227 |
32-37 |
NN |
denotes |
assay |
| T25228 |
37-38 |
. |
denotes |
. |
| T25229 |
38-219 |
sentence |
denotes |
Dissected testes were fixed in Bouin's fixative or phosphate-buffered formalin overnight at 4 °C, progressively dehydrated in a graded ethanol series, and embedded in paraffin wax. |
| T25230 |
39-48 |
VBN |
denotes |
Dissected |
| T25231 |
49-55 |
NNS |
denotes |
testes |
| T25233 |
56-60 |
VBD |
denotes |
were |
| T25232 |
61-66 |
VBN |
denotes |
fixed |
| T25234 |
67-69 |
IN |
denotes |
in |
| T25235 |
70-75 |
NNP |
denotes |
Bouin |
| T25237 |
75-77 |
POS |
denotes |
's |
| T25236 |
78-86 |
NN |
denotes |
fixative |
| T25238 |
87-89 |
CC |
denotes |
or |
| T25239 |
90-99 |
NN |
denotes |
phosphate |
| T25241 |
99-100 |
HYPH |
denotes |
- |
| T25240 |
100-108 |
VBN |
denotes |
buffered |
| T25242 |
109-117 |
NN |
denotes |
formalin |
| T25243 |
118-127 |
RB |
denotes |
overnight |
| T25244 |
128-130 |
IN |
denotes |
at |
| T25245 |
131-132 |
CD |
denotes |
4 |
| T25246 |
133-135 |
NN |
denotes |
°C |
| T25247 |
135-137 |
, |
denotes |
, |
| T25248 |
137-150 |
RB |
denotes |
progressively |
| T25249 |
151-161 |
VBN |
denotes |
dehydrated |
| T25250 |
162-164 |
IN |
denotes |
in |
| T25251 |
165-166 |
DT |
denotes |
a |
| T25253 |
167-173 |
VBN |
denotes |
graded |
| T25254 |
174-181 |
NN |
denotes |
ethanol |
| T25252 |
182-188 |
NN |
denotes |
series |
| T25255 |
188-190 |
, |
denotes |
, |
| T25256 |
190-193 |
CC |
denotes |
and |
| T25257 |
194-202 |
VBN |
denotes |
embedded |
| T25258 |
203-205 |
IN |
denotes |
in |
| T25259 |
206-214 |
NN |
denotes |
paraffin |
| T25260 |
215-218 |
NN |
denotes |
wax |
| T25261 |
218-219 |
. |
denotes |
. |
| T25262 |
219-308 |
sentence |
denotes |
Sections (6 μm) were deparaffinized, rehydrated, and stained with hematoxylin and eosin. |
| T25263 |
220-228 |
NNS |
denotes |
Sections |
| T25265 |
229-230 |
-LRB- |
denotes |
( |
| T25267 |
230-231 |
CD |
denotes |
6 |
| T25266 |
232-234 |
NNS |
denotes |
μm |
| T25268 |
234-235 |
-RRB- |
denotes |
) |
| T25269 |
236-240 |
VBD |
denotes |
were |
| T25264 |
241-255 |
VBN |
denotes |
deparaffinized |
| T25270 |
255-257 |
, |
denotes |
, |
| T25271 |
257-267 |
VBN |
denotes |
rehydrated |
| T25272 |
267-269 |
, |
denotes |
, |
| T25273 |
269-272 |
CC |
denotes |
and |
| T25274 |
273-280 |
VBN |
denotes |
stained |
| T25275 |
281-285 |
IN |
denotes |
with |
| T25276 |
286-297 |
NN |
denotes |
hematoxylin |
| T25277 |
298-301 |
CC |
denotes |
and |
| T25278 |
302-307 |
NN |
denotes |
eosin |
| T25279 |
307-308 |
. |
denotes |
. |
| T25280 |
308-471 |
sentence |
denotes |
For TUNEL analyses, deparaffinized sections were treated with proteinase K for 15 min and quenched in 3.0% hydrogen peroxide in PBS for 5 min at room temperature. |
| T25281 |
309-312 |
IN |
denotes |
For |
| T25283 |
313-318 |
NN |
denotes |
TUNEL |
| T25284 |
319-327 |
NNS |
denotes |
analyses |
| T25285 |
327-329 |
, |
denotes |
, |
| T25286 |
329-343 |
VBN |
denotes |
deparaffinized |
| T25287 |
344-352 |
NNS |
denotes |
sections |
| T25288 |
353-357 |
VBD |
denotes |
were |
| T25282 |
358-365 |
VBN |
denotes |
treated |
| T25289 |
366-370 |
IN |
denotes |
with |
| T25290 |
371-381 |
NN |
denotes |
proteinase |
| T25291 |
382-383 |
NN |
denotes |
K |
| T25292 |
384-387 |
IN |
denotes |
for |
| T25293 |
388-390 |
CD |
denotes |
15 |
| T25294 |
391-394 |
NNS |
denotes |
min |
| T25295 |
395-398 |
CC |
denotes |
and |
| T25296 |
399-407 |
VBN |
denotes |
quenched |
| T25297 |
408-410 |
IN |
denotes |
in |
| T25298 |
411-414 |
CD |
denotes |
3.0 |
| T25299 |
414-415 |
NN |
denotes |
% |
| T25301 |
416-424 |
NN |
denotes |
hydrogen |
| T25300 |
425-433 |
NN |
denotes |
peroxide |
| T25302 |
434-436 |
IN |
denotes |
in |
| T25303 |
437-440 |
NN |
denotes |
PBS |
| T25304 |
441-444 |
IN |
denotes |
for |
| T25305 |
445-446 |
CD |
denotes |
5 |
| T25306 |
447-450 |
NNS |
denotes |
min |
| T25307 |
451-453 |
IN |
denotes |
at |
| T25308 |
454-458 |
NN |
denotes |
room |
| T25309 |
459-470 |
NN |
denotes |
temperature |
| T25310 |
470-471 |
. |
denotes |
. |
| T25311 |
471-634 |
sentence |
denotes |
Subsequently, nuclear staining in apoptotic cells was detected using ApopTag kit (Chemicon, http://www.chemicon.com) according to the manufacturer's instructions. |
| T25312 |
472-484 |
RB |
denotes |
Subsequently |
| T25314 |
484-486 |
, |
denotes |
, |
| T25315 |
486-493 |
JJ |
denotes |
nuclear |
| T25316 |
494-502 |
NN |
denotes |
staining |
| T25317 |
503-505 |
IN |
denotes |
in |
| T25318 |
506-515 |
JJ |
denotes |
apoptotic |
| T25319 |
516-521 |
NNS |
denotes |
cells |
| T25320 |
522-525 |
VBD |
denotes |
was |
| T25313 |
526-534 |
VBN |
denotes |
detected |
| T25321 |
535-540 |
VBG |
denotes |
using |
| T25322 |
541-548 |
NN |
denotes |
ApopTag |
| T25323 |
549-552 |
NN |
denotes |
kit |
| T25324 |
553-554 |
-LRB- |
denotes |
( |
| T25325 |
554-562 |
NNP |
denotes |
Chemicon |
| T25326 |
562-564 |
, |
denotes |
, |
| T25327 |
564-587 |
NN |
denotes |
http://www.chemicon.com |
| T25328 |
587-588 |
-RRB- |
denotes |
) |
| T25329 |
589-598 |
VBG |
denotes |
according |
| T25330 |
599-601 |
IN |
denotes |
to |
| T25331 |
602-605 |
DT |
denotes |
the |
| T25332 |
606-618 |
NN |
denotes |
manufacturer |
| T25334 |
618-620 |
POS |
denotes |
's |
| T25333 |
621-633 |
NNS |
denotes |
instructions |
| T25335 |
633-634 |
. |
denotes |
. |
| R6997 |
T25223 |
T25224 |
amod |
Histological,analysis |
| R6998 |
T25225 |
T25224 |
cc |
and,analysis |
| R6999 |
T25226 |
T25227 |
compound |
TUNEL,assay |
| R7000 |
T25227 |
T25224 |
conj |
assay,analysis |
| R7001 |
T25228 |
T25227 |
punct |
.,assay |
| R7002 |
T25230 |
T25231 |
amod |
Dissected,testes |
| R7003 |
T25231 |
T25232 |
nsubjpass |
testes,fixed |
| R7004 |
T25233 |
T25232 |
auxpass |
were,fixed |
| R7005 |
T25234 |
T25232 |
prep |
in,fixed |
| R7006 |
T25235 |
T25236 |
poss |
Bouin,fixative |
| R7007 |
T25236 |
T25234 |
pobj |
fixative,in |
| R7008 |
T25237 |
T25235 |
case |
's,Bouin |
| R7009 |
T25238 |
T25236 |
cc |
or,fixative |
| R7010 |
T25239 |
T25240 |
npadvmod |
phosphate,buffered |
| R7011 |
T25240 |
T25242 |
amod |
buffered,formalin |
| R7012 |
T25241 |
T25240 |
punct |
-,buffered |
| R7013 |
T25242 |
T25236 |
conj |
formalin,fixative |
| R7014 |
T25243 |
T25232 |
advmod |
overnight,fixed |
| R7015 |
T25244 |
T25232 |
prep |
at,fixed |
| R7016 |
T25245 |
T25246 |
nummod |
4,°C |
| R7017 |
T25246 |
T25244 |
pobj |
°C,at |
| R7018 |
T25247 |
T25232 |
punct |
", ",fixed |
| R7019 |
T25248 |
T25249 |
advmod |
progressively,dehydrated |
| R7020 |
T25249 |
T25232 |
conj |
dehydrated,fixed |
| R7021 |
T25250 |
T25249 |
prep |
in,dehydrated |
| R7022 |
T25251 |
T25252 |
det |
a,series |
| R7023 |
T25252 |
T25250 |
pobj |
series,in |
| R7024 |
T25253 |
T25252 |
amod |
graded,series |
| R7025 |
T25254 |
T25252 |
compound |
ethanol,series |
| R7026 |
T25255 |
T25249 |
punct |
", ",dehydrated |
| R7027 |
T25256 |
T25249 |
cc |
and,dehydrated |
| R7028 |
T25257 |
T25249 |
conj |
embedded,dehydrated |
| R7029 |
T25258 |
T25257 |
prep |
in,embedded |
| R7030 |
T25259 |
T25260 |
compound |
paraffin,wax |
| R7031 |
T25260 |
T25258 |
pobj |
wax,in |
| R7032 |
T25261 |
T25232 |
punct |
.,fixed |
| R7033 |
T25263 |
T25264 |
nsubjpass |
Sections,deparaffinized |
| R7034 |
T25265 |
T25266 |
punct |
(,μm |
| R7035 |
T25266 |
T25263 |
parataxis |
μm,Sections |
| R7036 |
T25267 |
T25266 |
nummod |
6,μm |
| R7037 |
T25268 |
T25266 |
punct |
),μm |
| R7038 |
T25269 |
T25264 |
auxpass |
were,deparaffinized |
| R7039 |
T25270 |
T25264 |
punct |
", ",deparaffinized |
| R7040 |
T25271 |
T25264 |
conj |
rehydrated,deparaffinized |
| R7041 |
T25272 |
T25271 |
punct |
", ",rehydrated |
| R7042 |
T25273 |
T25271 |
cc |
and,rehydrated |
| R7043 |
T25274 |
T25271 |
conj |
stained,rehydrated |
| R7044 |
T25275 |
T25274 |
prep |
with,stained |
| R7045 |
T25276 |
T25275 |
pobj |
hematoxylin,with |
| R7046 |
T25277 |
T25276 |
cc |
and,hematoxylin |
| R7047 |
T25278 |
T25276 |
conj |
eosin,hematoxylin |
| R7048 |
T25279 |
T25264 |
punct |
.,deparaffinized |
| R7049 |
T25281 |
T25282 |
prep |
For,treated |
| R7050 |
T25283 |
T25284 |
compound |
TUNEL,analyses |
| R7051 |
T25284 |
T25281 |
pobj |
analyses,For |
| R7052 |
T25285 |
T25282 |
punct |
", ",treated |
| R7053 |
T25286 |
T25287 |
amod |
deparaffinized,sections |
| R7054 |
T25287 |
T25282 |
nsubjpass |
sections,treated |
| R7055 |
T25288 |
T25282 |
auxpass |
were,treated |
| R7056 |
T25289 |
T25282 |
prep |
with,treated |
| R7057 |
T25290 |
T25291 |
compound |
proteinase,K |
| R7058 |
T25291 |
T25289 |
pobj |
K,with |
| R7059 |
T25292 |
T25282 |
prep |
for,treated |
| R7060 |
T25293 |
T25294 |
nummod |
15,min |
| R7061 |
T25294 |
T25292 |
pobj |
min,for |
| R7062 |
T25295 |
T25282 |
cc |
and,treated |
| R7063 |
T25296 |
T25282 |
conj |
quenched,treated |
| R7064 |
T25297 |
T25296 |
prep |
in,quenched |
| R7065 |
T25298 |
T25299 |
nummod |
3.0,% |
| R7066 |
T25299 |
T25300 |
compound |
%,peroxide |
| R7067 |
T25300 |
T25297 |
pobj |
peroxide,in |
| R7068 |
T25301 |
T25300 |
compound |
hydrogen,peroxide |
| R7069 |
T25302 |
T25296 |
prep |
in,quenched |
| R7070 |
T25303 |
T25302 |
pobj |
PBS,in |
| R7071 |
T25304 |
T25296 |
prep |
for,quenched |
| R7072 |
T25305 |
T25306 |
nummod |
5,min |
| R7073 |
T25306 |
T25304 |
pobj |
min,for |
| R7074 |
T25307 |
T25296 |
prep |
at,quenched |
| R7075 |
T25308 |
T25309 |
compound |
room,temperature |
| R7076 |
T25309 |
T25307 |
pobj |
temperature,at |
| R7077 |
T25310 |
T25282 |
punct |
.,treated |
| R7078 |
T25312 |
T25313 |
advmod |
Subsequently,detected |
| R7079 |
T25314 |
T25313 |
punct |
", ",detected |
| R7080 |
T25315 |
T25316 |
amod |
nuclear,staining |
| R7081 |
T25316 |
T25313 |
nsubjpass |
staining,detected |
| R7082 |
T25317 |
T25316 |
prep |
in,staining |
| R7083 |
T25318 |
T25319 |
amod |
apoptotic,cells |
| R7084 |
T25319 |
T25317 |
pobj |
cells,in |
| R7085 |
T25320 |
T25313 |
auxpass |
was,detected |
| R7086 |
T25321 |
T25313 |
advcl |
using,detected |
| R7087 |
T25322 |
T25323 |
compound |
ApopTag,kit |
| R7088 |
T25323 |
T25321 |
dobj |
kit,using |
| R7089 |
T25324 |
T25325 |
punct |
(,Chemicon |
| R7090 |
T25325 |
T25323 |
parataxis |
Chemicon,kit |
| R7091 |
T25326 |
T25325 |
punct |
", ",Chemicon |
| R7092 |
T25327 |
T25325 |
npadvmod |
http://www.chemicon.com,Chemicon |
| R7093 |
T25328 |
T25325 |
punct |
),Chemicon |
| R7094 |
T25329 |
T25321 |
prep |
according,using |
| R7095 |
T25330 |
T25329 |
prep |
to,according |
| R7096 |
T25331 |
T25332 |
det |
the,manufacturer |
| R7097 |
T25332 |
T25333 |
poss |
manufacturer,instructions |
| R7098 |
T25333 |
T25330 |
pobj |
instructions,to |
| R7099 |
T25334 |
T25332 |
case |
's,manufacturer |
| R7100 |
T25335 |
T25313 |
punct |
.,detected |
