PMC:1713256 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T152	0-7	JJ	denotes	Genetic
T154	0-91	sentence	denotes	Genetic Analysis of the Roles of BMP2, BMP4, and BMP7 in Limb Patterning and Skeletogenesis
T153	8-16	NN	denotes	Analysis
T155	17-19	IN	denotes	of
T156	20-23	DT	denotes	the
T157	24-29	NNS	denotes	Roles
T158	30-32	IN	denotes	of
T159	33-37	NN	denotes	BMP2
T160	37-39	,	denotes	,
T161	39-43	NN	denotes	BMP4
T162	43-45	,	denotes	,
T163	45-48	CC	denotes	and
T164	49-53	NN	denotes	BMP7
T165	54-56	IN	denotes	in
T166	57-61	NN	denotes	Limb
T167	62-72	NN	denotes	Patterning
T168	73-76	CC	denotes	and
T169	77-91	NN	denotes	Skeletogenesis
T170	91-92	sentence	denotes
T172	135-139	NN	denotes	Bone
T171	135-258	sentence	denotes	Bone morphogenetic protein (BMP) family members, including BMP2, BMP4, and BMP7, are expressed throughout limb development.
T174	140-153	JJ	denotes	morphogenetic
T173	154-161	NN	denotes	protein
T176	162-163	-LRB-	denotes	(
T177	163-166	NN	denotes	BMP
T178	166-167	-RRB-	denotes	)
T179	168-174	NN	denotes	family
T175	175-182	NNS	denotes	members
T181	182-184	,	denotes	,
T182	184-193	VBG	denotes	including
T183	194-198	NN	denotes	BMP2
T184	198-200	,	denotes	,
T185	200-204	NN	denotes	BMP4
T186	204-206	,	denotes	,
T187	206-209	CC	denotes	and
T188	210-214	NN	denotes	BMP7
T189	214-216	,	denotes	,
T190	216-219	VBP	denotes	are
T180	220-229	VBN	denotes	expressed
T191	230-240	IN	denotes	throughout
T192	241-245	NN	denotes	limb
T193	246-257	NN	denotes	development
T194	257-258	.	denotes	.
T195	258-354	sentence	denotes	BMPs have been implicated in early limb patterning as well as in the process of skeletogenesis.
T196	259-263	NNS	denotes	BMPs
T198	264-268	VBP	denotes	have
T199	269-273	VBN	denotes	been
T197	274-284	VBN	denotes	implicated
T200	285-287	IN	denotes	in
T201	288-293	JJ	denotes	early
T203	294-298	NN	denotes	limb
T202	299-309	NN	denotes	patterning
T204	310-312	RB	denotes	as
T206	313-317	RB	denotes	well
T205	318-320	IN	denotes	as
T207	321-323	IN	denotes	in
T208	324-327	DT	denotes	the
T209	328-335	NN	denotes	process
T210	336-338	IN	denotes	of
T211	339-353	NN	denotes	skeletogenesis
T212	353-354	.	denotes	.
T213	354-632	sentence	denotes	However, due to complications associated with early embryonic lethality, particularly for Bmp2 and Bmp4, and with functional redundancy among BMP molecules, it has been difficult to decipher the specific roles of these BMP molecules during different stages of limb development.
T214	355-362	RB	denotes	However
T216	362-364	,	denotes	,
T217	364-367	IN	denotes	due
T218	368-370	IN	denotes	to
T219	371-384	NNS	denotes	complications
T220	385-395	VBN	denotes	associated
T221	396-400	IN	denotes	with
T222	401-406	JJ	denotes	early
T224	407-416	JJ	denotes	embryonic
T223	417-426	NN	denotes	lethality
T225	426-428	,	denotes	,
T226	428-440	RB	denotes	particularly
T227	441-444	IN	denotes	for
T228	445-449	NN	denotes	Bmp2
T229	450-453	CC	denotes	and
T230	454-458	NN	denotes	Bmp4
T231	458-460	,	denotes	,
T232	460-463	CC	denotes	and
T233	464-468	IN	denotes	with
T234	469-479	JJ	denotes	functional
T235	480-490	NN	denotes	redundancy
T236	491-496	IN	denotes	among
T237	497-500	NN	denotes	BMP
T238	501-510	NNS	denotes	molecules
T239	510-512	,	denotes	,
T240	512-514	PRP	denotes	it
T241	515-518	VBZ	denotes	has
T215	519-523	VBN	denotes	been
T242	524-533	JJ	denotes	difficult
T243	534-536	TO	denotes	to
T244	537-545	VB	denotes	decipher
T245	546-549	DT	denotes	the
T247	550-558	JJ	denotes	specific
T246	559-564	NNS	denotes	roles
T248	565-567	IN	denotes	of
T249	568-573	DT	denotes	these
T251	574-577	NN	denotes	BMP
T250	578-587	NNS	denotes	molecules
T252	588-594	IN	denotes	during
T253	595-604	JJ	denotes	different
T254	605-611	NNS	denotes	stages
T255	612-614	IN	denotes	of
T256	615-619	NN	denotes	limb
T257	620-631	NN	denotes	development
T258	631-632	.	denotes	.
T259	632-854	sentence	denotes	To circumvent these issues, we have constructed a series of mouse strains lacking one or more of these BMPs, using conditional alleles in the case of Bmp2 and Bmp4 to remove them specifically from the limb bud mesenchyme.
T260	633-635	TO	denotes	To
T261	636-646	VB	denotes	circumvent
T263	647-652	DT	denotes	these
T264	653-659	NNS	denotes	issues
T265	659-661	,	denotes	,
T266	661-663	PRP	denotes	we
T267	664-668	VBP	denotes	have
T262	669-680	VBN	denotes	constructed
T268	681-682	DT	denotes	a
T269	683-689	NN	denotes	series
T270	690-692	IN	denotes	of
T271	693-698	NN	denotes	mouse
T272	699-706	NNS	denotes	strains
T273	707-714	VBG	denotes	lacking
T274	715-718	CD	denotes	one
T275	719-721	CC	denotes	or
T276	722-726	JJR	denotes	more
T277	727-729	IN	denotes	of
T278	730-735	DT	denotes	these
T279	736-740	NNS	denotes	BMPs
T280	740-742	,	denotes	,
T281	742-747	VBG	denotes	using
T282	748-759	JJ	denotes	conditional
T283	760-767	NNS	denotes	alleles
T284	768-770	IN	denotes	in
T285	771-774	DT	denotes	the
T286	775-779	NN	denotes	case
T287	780-782	IN	denotes	of
T288	783-787	NN	denotes	Bmp2
T289	788-791	CC	denotes	and
T290	792-796	NN	denotes	Bmp4
T291	797-799	TO	denotes	to
T292	800-806	VB	denotes	remove
T293	807-811	PRP	denotes	them
T294	812-824	RB	denotes	specifically
T295	825-829	IN	denotes	from
T296	830-833	DT	denotes	the
T298	834-838	NN	denotes	limb
T299	839-842	NN	denotes	bud
T297	843-853	NN	denotes	mesenchyme
T300	853-854	.	denotes	.
T301	854-1102	sentence	denotes	Contrary to earlier suggestions, our results indicate that BMPs neither act as secondary signals downstream of Sonic Hedghog (SHH) in patterning the anteroposterior axis nor as signals from the interdigital mesenchyme in specifying digit identity.
T302	855-863	JJ	denotes	Contrary
T304	864-866	IN	denotes	to
T305	867-874	JJR	denotes	earlier
T306	875-886	NNS	denotes	suggestions
T307	886-888	,	denotes	,
T308	888-891	PRP$	denotes	our
T309	892-899	NNS	denotes	results
T303	900-908	VBP	denotes	indicate
T310	909-913	IN	denotes	that
T311	914-918	NNS	denotes	BMPs
T312	919-926	CC	denotes	neither
T313	927-930	VBP	denotes	act
T314	931-933	IN	denotes	as
T315	934-943	JJ	denotes	secondary
T316	944-951	NNS	denotes	signals
T317	952-962	RB	denotes	downstream
T318	963-965	IN	denotes	of
T319	966-971	JJ	denotes	Sonic
T320	972-979	NN	denotes	Hedghog
T321	980-981	-LRB-	denotes	(
T322	981-984	NN	denotes	SHH
T323	984-985	-RRB-	denotes	)
T324	986-988	IN	denotes	in
T325	989-999	VBG	denotes	patterning
T326	1000-1003	DT	denotes	the
T328	1004-1019	JJ	denotes	anteroposterior
T327	1020-1024	NN	denotes	axis
T329	1025-1028	CC	denotes	nor
T330	1029-1031	IN	denotes	as
T331	1032-1039	NNS	denotes	signals
T332	1040-1044	IN	denotes	from
T333	1045-1048	DT	denotes	the
T335	1049-1061	JJ	denotes	interdigital
T334	1062-1072	NN	denotes	mesenchyme
T336	1073-1075	IN	denotes	in
T337	1076-1086	VBG	denotes	specifying
T338	1087-1092	NN	denotes	digit
T339	1093-1101	NN	denotes	identity
T340	1101-1102	.	denotes	.
T341	1102-1295	sentence	denotes	We do find that a threshold level of BMP signaling is required for the onset of chondrogenesis, and hence some chondrogenic condensations fail to form in limbs deficient in both BMP2 and BMP4.
T342	1103-1105	PRP	denotes	We
T344	1106-1108	VBP	denotes	do
T343	1109-1113	VB	denotes	find
T345	1114-1118	IN	denotes	that
T347	1119-1120	DT	denotes	a
T349	1121-1130	NN	denotes	threshold
T348	1131-1136	NN	denotes	level
T350	1137-1139	IN	denotes	of
T351	1140-1143	NN	denotes	BMP
T352	1144-1153	NN	denotes	signaling
T353	1154-1156	VBZ	denotes	is
T346	1157-1165	VBN	denotes	required
T354	1166-1169	IN	denotes	for
T355	1170-1173	DT	denotes	the
T356	1174-1179	NN	denotes	onset
T357	1180-1182	IN	denotes	of
T358	1183-1197	NN	denotes	chondrogenesis
T359	1197-1199	,	denotes	,
T360	1199-1202	CC	denotes	and
T361	1203-1208	RB	denotes	hence
T363	1209-1213	DT	denotes	some
T365	1214-1226	JJ	denotes	chondrogenic
T364	1227-1240	NNS	denotes	condensations
T362	1241-1245	VBP	denotes	fail
T366	1246-1248	TO	denotes	to
T367	1249-1253	VB	denotes	form
T368	1254-1256	IN	denotes	in
T369	1257-1262	NNS	denotes	limbs
T370	1263-1272	JJ	denotes	deficient
T371	1273-1275	IN	denotes	in
T372	1276-1280	CC	denotes	both
T373	1281-1285	NN	denotes	BMP2
T374	1286-1289	CC	denotes	and
T375	1290-1294	NN	denotes	BMP4
T376	1294-1295	.	denotes	.
T377	1295-1452	sentence	denotes	However, in the condensations that do form, subsequent chondrogenic differentiation proceeds normally even in the absence of BMP2 and BMP7 or BMP2 and BMP4.
T378	1296-1303	RB	denotes	However
T380	1303-1305	,	denotes	,
T381	1305-1307	IN	denotes	in
T382	1308-1311	DT	denotes	the
T383	1312-1325	NNS	denotes	condensations
T384	1326-1330	WDT	denotes	that
T386	1331-1333	VBP	denotes	do
T385	1334-1338	VB	denotes	form
T387	1338-1340	,	denotes	,
T388	1340-1350	JJ	denotes	subsequent
T390	1351-1363	JJ	denotes	chondrogenic
T389	1364-1379	NN	denotes	differentiation
T379	1380-1388	VBZ	denotes	proceeds
T391	1389-1397	RB	denotes	normally
T392	1398-1402	RB	denotes	even
T393	1403-1405	IN	denotes	in
T394	1406-1409	DT	denotes	the
T395	1410-1417	NN	denotes	absence
T396	1418-1420	IN	denotes	of
T397	1421-1425	NN	denotes	BMP2
T398	1426-1429	CC	denotes	and
T399	1430-1434	NN	denotes	BMP7
T400	1435-1437	CC	denotes	or
T401	1438-1442	NN	denotes	BMP2
T402	1443-1446	CC	denotes	and
T403	1447-1451	NN	denotes	BMP4
T404	1451-1452	.	denotes	.
T405	1452-1557	sentence	denotes	In contrast, we find that the loss of both BMP2 and BMP4 results in a severe impairment of osteogenesis.
T406	1453-1455	IN	denotes	In
T408	1456-1464	NN	denotes	contrast
T409	1464-1466	,	denotes	,
T410	1466-1468	PRP	denotes	we
T407	1469-1473	VBP	denotes	find
T411	1474-1478	IN	denotes	that
T413	1479-1482	DT	denotes	the
T414	1483-1487	NN	denotes	loss
T415	1488-1490	IN	denotes	of
T416	1491-1495	CC	denotes	both
T417	1496-1500	NN	denotes	BMP2
T418	1501-1504	CC	denotes	and
T419	1505-1509	NN	denotes	BMP4
T412	1510-1517	VBZ	denotes	results
T420	1518-1520	IN	denotes	in
T421	1521-1522	DT	denotes	a
T423	1523-1529	JJ	denotes	severe
T422	1530-1540	NN	denotes	impairment
T424	1541-1543	IN	denotes	of
T425	1544-1556	NN	denotes	osteogenesis
T426	1556-1557	.	denotes	.
T1147	2850-2854	NN	denotes	Bone
T1149	2855-2868	JJ	denotes	morphogenetic
T1148	2869-2877	NN	denotes	proteins
T1151	2878-2879	-LRB-	denotes	(
T1152	2879-2883	NNS	denotes	BMPs
T1153	2883-2884	-RRB-	denotes	)
T1150	2885-2888	VBP	denotes	are
T1154	2889-2897	VBN	denotes	secreted
T1156	2898-2907	NN	denotes	signaling
T1155	2908-2917	NNS	denotes	molecules
T1157	2918-2927	VBG	denotes	belonging
T1158	2928-2930	IN	denotes	to
T1159	2931-2934	DT	denotes	the
T1161	2935-2947	VBG	denotes	transforming
T1162	2948-2954	NN	denotes	growth
T1164	2955-2961	NN	denotes	factor
T1163	2962-2963	NN	denotes	β
T1160	2964-2975	NN	denotes	superfamily
T1165	2975-2977	,	denotes	,
T1166	2977-2987	RB	denotes	originally
T1167	2988-2998	VBN	denotes	identified
T1168	2999-3001	IN	denotes	on
T1169	3002-3005	DT	denotes	the
T1170	3006-3011	NN	denotes	basis
T1171	3012-3014	IN	denotes	of
T1172	3015-3020	PRP$	denotes	their
T1173	3021-3028	NN	denotes	ability
T1174	3029-3031	TO	denotes	to
T1175	3032-3038	VB	denotes	induce
T1176	3039-3046	JJ	denotes	ectopic
T1178	3047-3051	NN	denotes	bone
T1177	3052-3061	NN	denotes	formation
T1179	3062-3066	WRB	denotes	when
T1180	3067-3076	VBN	denotes	implanted
T1181	3077-3083	IN	denotes	within
T1182	3084-3088	JJ	denotes	soft
T1183	3089-3095	NN	denotes	tissue
T1184	3096-3098	FW	denotes	in
T1185	3099-3103	FW	denotes	vivo
T1186	3104-3105	-LRB-	denotes	[
T1187	3105-3106	CD	denotes	1
T1188	3106-3107	SYM	denotes	–
T1189	3107-3108	CD	denotes	3
T1190	3108-3109	-RRB-	denotes	]
T1191	3109-3110	.	denotes	.
T1192	3110-3235	sentence	denotes	BMP family members are now known to play an extremely diverse set of roles in a wide variety of developmental processes [4].
T1193	3111-3114	NN	denotes	BMP
T1195	3115-3121	NN	denotes	family
T1194	3122-3129	NNS	denotes	members
T1197	3130-3133	VBP	denotes	are
T1198	3134-3137	RB	denotes	now
T1196	3138-3143	VBN	denotes	known
T1199	3144-3146	TO	denotes	to
T1200	3147-3151	VB	denotes	play
T1201	3152-3154	DT	denotes	an
T1203	3155-3164	RB	denotes	extremely
T1204	3165-3172	JJ	denotes	diverse
T1202	3173-3176	NN	denotes	set
T1205	3177-3179	IN	denotes	of
T1206	3180-3185	NNS	denotes	roles
T1207	3186-3188	IN	denotes	in
T1208	3189-3190	DT	denotes	a
T1210	3191-3195	JJ	denotes	wide
T1209	3196-3203	NN	denotes	variety
T1211	3204-3206	IN	denotes	of
T1212	3207-3220	JJ	denotes	developmental
T1213	3221-3230	NNS	denotes	processes
T1214	3231-3232	-LRB-	denotes	[
T1215	3232-3233	CD	denotes	4
T1216	3233-3234	-RRB-	denotes	]
T1217	3234-3235	.	denotes	.
T1218	3235-3359	sentence	denotes	Even in the context of the morphogenesis of a single structure, these molecules can play a series of quite divergent roles.
T1219	3236-3240	RB	denotes	Even
T1220	3241-3243	IN	denotes	in
T1222	3244-3247	DT	denotes	the
T1223	3248-3255	NN	denotes	context
T1224	3256-3258	IN	denotes	of
T1225	3259-3262	DT	denotes	the
T1226	3263-3276	NN	denotes	morphogenesis
T1227	3277-3279	IN	denotes	of
T1228	3280-3281	DT	denotes	a
T1230	3282-3288	JJ	denotes	single
T1229	3289-3298	NN	denotes	structure
T1231	3298-3300	,	denotes	,
T1232	3300-3305	DT	denotes	these
T1233	3306-3315	NNS	denotes	molecules
T1234	3316-3319	MD	denotes	can
T1221	3320-3324	VB	denotes	play
T1235	3325-3326	DT	denotes	a
T1236	3327-3333	NN	denotes	series
T1237	3334-3336	IN	denotes	of
T1238	3337-3342	RB	denotes	quite
T1240	3343-3352	JJ	denotes	divergent
T1239	3353-3358	NNS	denotes	roles
T1241	3358-3359	.	denotes	.
T1242	3359-3582	sentence	denotes	For example, during limb development, BMPs have been postulated to act sequentially in multiple distinct aspects of patterning, cell type specification, and differentiation of various tissues, particularly of the skeleton.
T1243	3360-3363	IN	denotes	For
T1245	3364-3371	NN	denotes	example
T1246	3371-3373	,	denotes	,
T1247	3373-3379	IN	denotes	during
T1248	3380-3384	NN	denotes	limb
T1249	3385-3396	NN	denotes	development
T1250	3396-3398	,	denotes	,
T1251	3398-3402	NNS	denotes	BMPs
T1252	3403-3407	VBP	denotes	have
T1253	3408-3412	VBN	denotes	been
T1244	3413-3423	VBN	denotes	postulated
T1254	3424-3426	TO	denotes	to
T1255	3427-3430	VB	denotes	act
T1256	3431-3443	RB	denotes	sequentially
T1257	3444-3446	IN	denotes	in
T1258	3447-3455	JJ	denotes	multiple
T1260	3456-3464	JJ	denotes	distinct
T1259	3465-3472	NNS	denotes	aspects
T1261	3473-3475	IN	denotes	of
T1262	3476-3486	NN	denotes	patterning
T1263	3486-3488	,	denotes	,
T1264	3488-3492	NN	denotes	cell
T1265	3493-3497	NN	denotes	type
T1266	3498-3511	NN	denotes	specification
T1267	3511-3513	,	denotes	,
T1268	3513-3516	CC	denotes	and
T1269	3517-3532	NN	denotes	differentiation
T1270	3533-3535	IN	denotes	of
T1271	3536-3543	JJ	denotes	various
T1272	3544-3551	NNS	denotes	tissues
T1273	3551-3553	,	denotes	,
T1274	3553-3565	RB	denotes	particularly
T1275	3566-3568	IN	denotes	of
T1276	3569-3572	DT	denotes	the
T1277	3573-3581	NN	denotes	skeleton
T1278	3581-3582	.	denotes	.
T1279	3582-3721	sentence	denotes	The earliest step of limb development in which BMP signaling has been implicated is the establishment of the anterior-posterior limb axis.
T1280	3583-3586	DT	denotes	The
T1282	3587-3595	JJS	denotes	earliest
T1281	3596-3600	NN	denotes	step
T1284	3601-3603	IN	denotes	of
T1285	3604-3608	NN	denotes	limb
T1286	3609-3620	NN	denotes	development
T1287	3621-3623	IN	denotes	in
T1289	3624-3629	WDT	denotes	which
T1290	3630-3633	NN	denotes	BMP
T1291	3634-3643	NN	denotes	signaling
T1292	3644-3647	VBZ	denotes	has
T1293	3648-3652	VBN	denotes	been
T1288	3653-3663	VBN	denotes	implicated
T1283	3664-3666	VBZ	denotes	is
T1294	3667-3670	DT	denotes	the
T1295	3671-3684	NN	denotes	establishment
T1296	3685-3687	IN	denotes	of
T1297	3688-3691	DT	denotes	the
T1299	3692-3700	JJ	denotes	anterior
T1301	3700-3701	HYPH	denotes	-
T1300	3701-3710	JJ	denotes	posterior
T1302	3711-3715	NN	denotes	limb
T1298	3716-3720	NN	denotes	axis
T1303	3720-3721	.	denotes	.
T1304	3721-3889	sentence	denotes	Differences in anterior-posterior pattern are instructed as a graded response to Sonic Hedghog (SHH) signaling emanating from the posterior margin of the limb bud [5].
T1305	3722-3733	NNS	denotes	Differences
T1307	3734-3736	IN	denotes	in
T1308	3737-3745	JJ	denotes	anterior
T1310	3745-3746	HYPH	denotes	-
T1309	3746-3755	JJ	denotes	posterior
T1311	3756-3763	NN	denotes	pattern
T1312	3764-3767	VBP	denotes	are
T1306	3768-3778	VBN	denotes	instructed
T1313	3779-3781	IN	denotes	as
T1314	3782-3783	DT	denotes	a
T1316	3784-3790	VBN	denotes	graded
T1315	3791-3799	NN	denotes	response
T1317	3800-3802	IN	denotes	to
T1318	3803-3808	JJ	denotes	Sonic
T1319	3809-3816	NN	denotes	Hedghog
T1321	3817-3818	-LRB-	denotes	(
T1322	3818-3821	NN	denotes	SHH
T1323	3821-3822	-RRB-	denotes	)
T1320	3823-3832	NN	denotes	signaling
T1324	3833-3842	VBG	denotes	emanating
T1325	3843-3847	IN	denotes	from
T1326	3848-3851	DT	denotes	the
T1328	3852-3861	JJ	denotes	posterior
T1327	3862-3868	NN	denotes	margin
T1329	3869-3871	IN	denotes	of
T1330	3872-3875	DT	denotes	the
T1332	3876-3880	NN	denotes	limb
T1331	3881-3884	NN	denotes	bud
T1333	3885-3886	-LRB-	denotes	[
T1334	3886-3887	CD	denotes	5
T1335	3887-3888	-RRB-	denotes	]
T1336	3888-3889	.	denotes	.
T1337	3889-3974	sentence	denotes	It has remained controversial, however, whether this response is direct or indirect.
T1338	3890-3892	PRP	denotes	It
T1340	3893-3896	VBZ	denotes	has
T1339	3897-3905	VBN	denotes	remained
T1341	3906-3919	JJ	denotes	controversial
T1342	3919-3921	,	denotes	,
T1343	3921-3928	RB	denotes	however
T1344	3928-3930	,	denotes	,
T1345	3930-3937	IN	denotes	whether
T1347	3938-3942	DT	denotes	this
T1348	3943-3951	NN	denotes	response
T1346	3952-3954	VBZ	denotes	is
T1349	3955-3961	JJ	denotes	direct
T1350	3962-3964	CC	denotes	or
T1351	3965-3973	JJ	denotes	indirect
T1352	3973-3974	.	denotes	.
T1353	3974-4159	sentence	denotes	If indeed the long-range effects of SHH are indirectly mediated by local production of secondary signals, the leading candidates have been two members of the BMP family, BMP2 and BMP7.
T1354	3975-3977	IN	denotes	If
T1356	3978-3984	RB	denotes	indeed
T1357	3985-3988	DT	denotes	the
T1359	3989-3993	JJ	denotes	long
T1361	3993-3994	HYPH	denotes	-
T1360	3994-3999	NN	denotes	range
T1358	4000-4007	NNS	denotes	effects
T1362	4008-4010	IN	denotes	of
T1363	4011-4014	NN	denotes	SHH
T1364	4015-4018	VBP	denotes	are
T1365	4019-4029	RB	denotes	indirectly
T1355	4030-4038	VBN	denotes	mediated
T1367	4039-4041	IN	denotes	by
T1368	4042-4047	JJ	denotes	local
T1369	4048-4058	NN	denotes	production
T1370	4059-4061	IN	denotes	of
T1371	4062-4071	JJ	denotes	secondary
T1372	4072-4079	NNS	denotes	signals
T1373	4079-4081	,	denotes	,
T1374	4081-4084	DT	denotes	the
T1376	4085-4092	VBG	denotes	leading
T1375	4093-4103	NNS	denotes	candidates
T1377	4104-4108	VBP	denotes	have
T1366	4109-4113	VBN	denotes	been
T1378	4114-4117	CD	denotes	two
T1379	4118-4125	NNS	denotes	members
T1380	4126-4128	IN	denotes	of
T1381	4129-4132	DT	denotes	the
T1383	4133-4136	NN	denotes	BMP
T1382	4137-4143	NN	denotes	family
T1384	4143-4145	,	denotes	,
T1385	4145-4149	NN	denotes	BMP2
T1386	4150-4153	CC	denotes	and
T1387	4154-4158	NN	denotes	BMP7
T1388	4158-4159	.	denotes	.
T1389	4159-4355	sentence	denotes	Both are expressed in a broader domain than SHH in the early posterior limb bud mesenchyme [6,7], although BMP7 also has a second, weaker domain of expression in the anterior limb bud mesenchyme.
T1390	4160-4164	DT	denotes	Both
T1392	4165-4168	VBP	denotes	are
T1391	4169-4178	VBN	denotes	expressed
T1393	4179-4181	IN	denotes	in
T1394	4182-4183	DT	denotes	a
T1396	4184-4191	JJR	denotes	broader
T1395	4192-4198	NN	denotes	domain
T1397	4199-4203	IN	denotes	than
T1398	4204-4207	NN	denotes	SHH
T1399	4208-4210	IN	denotes	in
T1400	4211-4214	DT	denotes	the
T1402	4215-4220	JJ	denotes	early
T1404	4221-4230	JJ	denotes	posterior
T1405	4231-4235	NN	denotes	limb
T1403	4236-4239	NN	denotes	bud
T1401	4240-4250	NN	denotes	mesenchyme
T1406	4251-4252	-LRB-	denotes	[
T1408	4252-4253	CD	denotes	6
T1409	4253-4254	,	denotes	,
T1407	4254-4255	CD	denotes	7
T1410	4255-4256	-RRB-	denotes	]
T1411	4256-4258	,	denotes	,
T1412	4258-4266	IN	denotes	although
T1414	4267-4271	NN	denotes	BMP7
T1415	4272-4276	RB	denotes	also
T1413	4277-4280	VBZ	denotes	has
T1416	4281-4282	DT	denotes	a
T1418	4283-4289	JJ	denotes	second
T1419	4289-4291	,	denotes	,
T1420	4291-4297	JJR	denotes	weaker
T1417	4298-4304	NN	denotes	domain
T1421	4305-4307	IN	denotes	of
T1422	4308-4318	NN	denotes	expression
T1423	4319-4321	IN	denotes	in
T1424	4322-4325	DT	denotes	the
T1426	4326-4334	JJ	denotes	anterior
T1428	4335-4339	NN	denotes	limb
T1427	4340-4343	NN	denotes	bud
T1425	4344-4354	NN	denotes	mesenchyme
T1429	4354-4355	.	denotes	.
T1430	4355-4486	sentence	denotes	BMP2 [8] and BMP7 [7] can be induced by ectopic SHH and their expression is greatly diminished in the absence of SHH activity [9].
T1431	4356-4360	NN	denotes	BMP2
T1433	4361-4362	-LRB-	denotes	[
T1434	4362-4363	CD	denotes	8
T1435	4363-4364	-RRB-	denotes	]
T1436	4365-4368	CC	denotes	and
T1437	4369-4373	NN	denotes	BMP7
T1438	4374-4375	-LRB-	denotes	[
T1439	4375-4376	CD	denotes	7
T1440	4376-4377	-RRB-	denotes	]
T1441	4378-4381	MD	denotes	can
T1442	4382-4384	VB	denotes	be
T1432	4385-4392	VBN	denotes	induced
T1443	4393-4395	IN	denotes	by
T1444	4396-4403	JJ	denotes	ectopic
T1445	4404-4407	NN	denotes	SHH
T1446	4408-4411	CC	denotes	and
T1447	4412-4417	PRP$	denotes	their
T1448	4418-4428	NN	denotes	expression
T1450	4429-4431	VBZ	denotes	is
T1451	4432-4439	RB	denotes	greatly
T1449	4440-4450	VBN	denotes	diminished
T1452	4451-4453	IN	denotes	in
T1453	4454-4457	DT	denotes	the
T1454	4458-4465	NN	denotes	absence
T1455	4466-4468	IN	denotes	of
T1456	4469-4472	NN	denotes	SHH
T1457	4473-4481	NN	denotes	activity
T1458	4482-4483	-LRB-	denotes	[
T1459	4483-4484	CD	denotes	9
T1460	4484-4485	-RRB-	denotes	]
T1461	4485-4486	.	denotes	.
T1462	4486-4565	sentence	denotes	BMP2 and BMP7 are thus secondary signals produced in response to SHH activity.
T1463	4487-4491	NN	denotes	BMP2
T1465	4492-4495	CC	denotes	and
T1466	4496-4500	NN	denotes	BMP7
T1464	4501-4504	VBP	denotes	are
T1467	4505-4509	RB	denotes	thus
T1468	4510-4519	JJ	denotes	secondary
T1469	4520-4527	NNS	denotes	signals
T1470	4528-4536	VBN	denotes	produced
T1471	4537-4539	IN	denotes	in
T1472	4540-4548	NN	denotes	response
T1473	4549-4551	IN	denotes	to
T1474	4552-4555	NN	denotes	SHH
T1475	4556-4564	NN	denotes	activity
T1476	4564-4565	.	denotes	.
T1477	4565-4741	sentence	denotes	Moreover, BMP signaling has a weak ability to posteriorly polarize the limb in ectopic grafting experiments [10], an activity enhanced by prior low-level exposure to SHH [11].
T1478	4566-4574	RB	denotes	Moreover
T1480	4574-4576	,	denotes	,
T1481	4576-4579	NN	denotes	BMP
T1482	4580-4589	NN	denotes	signaling
T1479	4590-4593	VBZ	denotes	has
T1483	4594-4595	DT	denotes	a
T1485	4596-4600	JJ	denotes	weak
T1484	4601-4608	NN	denotes	ability
T1486	4609-4611	TO	denotes	to
T1488	4612-4623	RB	denotes	posteriorly
T1487	4624-4632	VB	denotes	polarize
T1489	4633-4636	DT	denotes	the
T1490	4637-4641	NN	denotes	limb
T1491	4642-4644	IN	denotes	in
T1492	4645-4652	JJ	denotes	ectopic
T1494	4653-4661	NN	denotes	grafting
T1493	4662-4673	NNS	denotes	experiments
T1495	4674-4675	-LRB-	denotes	[
T1496	4675-4677	CD	denotes	10
T1497	4677-4678	-RRB-	denotes	]
T1498	4678-4680	,	denotes	,
T1499	4680-4682	DT	denotes	an
T1500	4683-4691	NN	denotes	activity
T1501	4692-4700	VBN	denotes	enhanced
T1502	4701-4703	IN	denotes	by
T1503	4704-4709	JJ	denotes	prior
T1505	4710-4713	JJ	denotes	low
T1507	4713-4714	HYPH	denotes	-
T1506	4714-4719	NN	denotes	level
T1504	4720-4728	NN	denotes	exposure
T1508	4729-4731	IN	denotes	to
T1509	4732-4735	NN	denotes	SHH
T1510	4736-4737	-LRB-	denotes	[
T1511	4737-4739	CD	denotes	11
T1512	4739-4740	-RRB-	denotes	]
T1513	4740-4741	.	denotes	.
T1514	4741-4873	sentence	denotes	It remains unclear, however, whether BMP2 and BMP7 activity is required endogenously for anterior-posterior limb patterning by SHH.
T1515	4742-4744	PRP	denotes	It
T1516	4745-4752	VBZ	denotes	remains
T1517	4753-4760	JJ	denotes	unclear
T1518	4760-4762	,	denotes	,
T1519	4762-4769	RB	denotes	however
T1520	4769-4771	,	denotes	,
T1521	4771-4778	IN	denotes	whether
T1523	4779-4783	NN	denotes	BMP2
T1525	4784-4787	CC	denotes	and
T1526	4788-4792	NN	denotes	BMP7
T1524	4793-4801	NN	denotes	activity
T1527	4802-4804	VBZ	denotes	is
T1522	4805-4813	VBN	denotes	required
T1528	4814-4826	RB	denotes	endogenously
T1529	4827-4830	IN	denotes	for
T1530	4831-4839	JJ	denotes	anterior
T1532	4839-4840	HYPH	denotes	-
T1531	4840-4849	JJ	denotes	posterior
T1534	4850-4854	NN	denotes	limb
T1533	4855-4865	NN	denotes	patterning
T1535	4866-4868	IN	denotes	by
T1536	4869-4872	NN	denotes	SHH
T1537	4872-4873	.	denotes	.
T1538	4873-4940	sentence	denotes	Bmp2 mutant embryos die too early to assess their limb phenotypes.
T1539	4874-4878	NN	denotes	Bmp2
T1541	4879-4885	NN	denotes	mutant
T1540	4886-4893	NNS	denotes	embryos
T1542	4894-4897	VBP	denotes	die
T1543	4898-4901	RB	denotes	too
T1544	4902-4907	RB	denotes	early
T1545	4908-4910	TO	denotes	to
T1546	4911-4917	VB	denotes	assess
T1547	4918-4923	PRP$	denotes	their
T1549	4924-4928	NN	denotes	limb
T1548	4929-4939	NNS	denotes	phenotypes
T1550	4939-4940	.	denotes	.
T1551	4940-5117	sentence	denotes	A targeted deletion of Bmp7 has been made, and Bmp7-deficient embryos display hindlimb polydactyly with incomplete penetrance but otherwise phenotypically normal limbs [12,13].
T1552	4941-4942	DT	denotes	A
T1554	4943-4951	VBN	denotes	targeted
T1553	4952-4960	NN	denotes	deletion
T1556	4961-4963	IN	denotes	of
T1557	4964-4968	NN	denotes	Bmp7
T1558	4969-4972	VBZ	denotes	has
T1559	4973-4977	VBN	denotes	been
T1555	4978-4982	VBN	denotes	made
T1560	4982-4984	,	denotes	,
T1561	4984-4987	CC	denotes	and
T1562	4988-4992	NN	denotes	Bmp7
T1564	4992-4993	HYPH	denotes	-
T1563	4993-5002	JJ	denotes	deficient
T1565	5003-5010	NNS	denotes	embryos
T1566	5011-5018	VBP	denotes	display
T1567	5019-5027	NN	denotes	hindlimb
T1568	5028-5039	NN	denotes	polydactyly
T1569	5040-5044	IN	denotes	with
T1570	5045-5055	JJ	denotes	incomplete
T1571	5056-5066	NN	denotes	penetrance
T1572	5067-5070	CC	denotes	but
T1573	5071-5080	RB	denotes	otherwise
T1575	5081-5095	RB	denotes	phenotypically
T1574	5096-5102	JJ	denotes	normal
T1576	5103-5108	NNS	denotes	limbs
T1577	5109-5110	-LRB-	denotes	[
T1579	5110-5112	CD	denotes	12
T1580	5112-5113	,	denotes	,
T1578	5113-5115	CD	denotes	13
T1581	5115-5116	-RRB-	denotes	]
T1582	5116-5117	.	denotes	.
T1583	5117-5190	sentence	denotes	Nonetheless, Bmp7 knockout mice do not show any defect in limb polarity.
T1584	5118-5129	RB	denotes	Nonetheless
T1586	5129-5131	,	denotes	,
T1587	5131-5135	NN	denotes	Bmp7
T1588	5136-5144	NN	denotes	knockout
T1589	5145-5149	NNS	denotes	mice
T1590	5150-5152	VBP	denotes	do
T1591	5153-5156	RB	denotes	not
T1585	5157-5161	VB	denotes	show
T1592	5162-5165	DT	denotes	any
T1593	5166-5172	NN	denotes	defect
T1594	5173-5175	IN	denotes	in
T1595	5176-5180	NN	denotes	limb
T1596	5181-5189	NN	denotes	polarity
T1597	5189-5190	.	denotes	.
T1598	5190-5286	sentence	denotes	However, a redundant function in anterior-posterior patterning with BMP2 remains a possibility.
T1599	5191-5198	RB	denotes	However
T1601	5198-5200	,	denotes	,
T1602	5200-5201	DT	denotes	a
T1604	5202-5211	JJ	denotes	redundant
T1603	5212-5220	NN	denotes	function
T1605	5221-5223	IN	denotes	in
T1606	5224-5232	JJ	denotes	anterior
T1608	5232-5233	HYPH	denotes	-
T1607	5233-5242	JJ	denotes	posterior
T1609	5243-5253	NN	denotes	patterning
T1610	5254-5258	IN	denotes	with
T1611	5259-5263	NN	denotes	BMP2
T1600	5264-5271	VBZ	denotes	remains
T1612	5272-5273	DT	denotes	a
T1613	5274-5285	NN	denotes	possibility
T1614	5285-5286	.	denotes	.
T1615	5286-5426	sentence	denotes	In addition to BMP2 and BMP7, a third member of this family that is closely related to BMP2, BMP4, is also expressed in the early limb bud.
T1616	5287-5289	IN	denotes	In
T1618	5290-5298	NN	denotes	addition
T1619	5299-5301	IN	denotes	to
T1620	5302-5306	NN	denotes	BMP2
T1621	5307-5310	CC	denotes	and
T1622	5311-5315	NN	denotes	BMP7
T1623	5315-5317	,	denotes	,
T1624	5317-5318	DT	denotes	a
T1626	5319-5324	JJ	denotes	third
T1625	5325-5331	NN	denotes	member
T1627	5332-5334	IN	denotes	of
T1628	5335-5339	DT	denotes	this
T1629	5340-5346	NN	denotes	family
T1630	5347-5351	WDT	denotes	that
T1632	5352-5354	VBZ	denotes	is
T1633	5355-5362	RB	denotes	closely
T1631	5363-5370	VBN	denotes	related
T1634	5371-5373	IN	denotes	to
T1635	5374-5378	NN	denotes	BMP2
T1636	5378-5380	,	denotes	,
T1637	5380-5384	NN	denotes	BMP4
T1638	5384-5386	,	denotes	,
T1639	5386-5388	VBZ	denotes	is
T1640	5389-5393	RB	denotes	also
T1617	5394-5403	VBN	denotes	expressed
T1641	5404-5406	IN	denotes	in
T1642	5407-5410	DT	denotes	the
T1644	5411-5416	JJ	denotes	early
T1645	5417-5421	NN	denotes	limb
T1643	5422-5425	NN	denotes	bud
T1646	5425-5426	.	denotes	.
T1647	5426-5650	sentence	denotes	Like BMP7, it is expressed in both the anterior and posterior margins of the limb bud mesenchyme [4,14]; however, it does not appear to be induced by SHH signaling, nor does its expression change in SHH-deficient limb buds.
T1648	5427-5431	IN	denotes	Like
T1650	5432-5436	NN	denotes	BMP7
T1651	5436-5438	,	denotes	,
T1652	5438-5440	PRP	denotes	it
T1653	5441-5443	VBZ	denotes	is
T1649	5444-5453	VBN	denotes	expressed
T1655	5454-5456	IN	denotes	in
T1656	5457-5461	CC	denotes	both
T1658	5462-5465	DT	denotes	the
T1659	5466-5474	JJ	denotes	anterior
T1660	5475-5478	CC	denotes	and
T1661	5479-5488	JJ	denotes	posterior
T1657	5489-5496	NNS	denotes	margins
T1662	5497-5499	IN	denotes	of
T1663	5500-5503	DT	denotes	the
T1665	5504-5508	NN	denotes	limb
T1666	5509-5512	NN	denotes	bud
T1664	5513-5523	NN	denotes	mesenchyme
T1667	5524-5525	-LRB-	denotes	[
T1669	5525-5526	CD	denotes	4
T1670	5526-5527	,	denotes	,
T1668	5527-5529	CD	denotes	14
T1671	5529-5530	-RRB-	denotes	]
T1672	5530-5531	:	denotes	;
T1673	5532-5539	RB	denotes	however
T1674	5539-5541	,	denotes	,
T1675	5541-5543	PRP	denotes	it
T1676	5544-5548	VBZ	denotes	does
T1677	5549-5552	RB	denotes	not
T1654	5553-5559	VB	denotes	appear
T1678	5560-5562	TO	denotes	to
T1680	5563-5565	VB	denotes	be
T1679	5566-5573	VBN	denotes	induced
T1681	5574-5576	IN	denotes	by
T1682	5577-5580	NN	denotes	SHH
T1683	5581-5590	NN	denotes	signaling
T1684	5590-5592	,	denotes	,
T1685	5592-5595	CC	denotes	nor
T1686	5596-5600	VBZ	denotes	does
T1688	5601-5604	PRP$	denotes	its
T1689	5605-5615	NN	denotes	expression
T1687	5616-5622	VB	denotes	change
T1690	5623-5625	IN	denotes	in
T1691	5626-5629	NN	denotes	SHH
T1693	5629-5630	HYPH	denotes	-
T1692	5630-5639	JJ	denotes	deficient
T1695	5640-5644	NN	denotes	limb
T1694	5645-5649	NNS	denotes	buds
T1696	5649-5650	.	denotes	.
T1697	5650-5742	sentence	denotes	Thus, BMP4 is not a candidate for a secondary signal downstream of SHH in early patterning.
T1698	5651-5655	RB	denotes	Thus
T1700	5655-5657	,	denotes	,
T1701	5657-5661	NN	denotes	BMP4
T1699	5662-5664	VBZ	denotes	is
T1702	5665-5668	RB	denotes	not
T1703	5669-5670	DT	denotes	a
T1704	5671-5680	NN	denotes	candidate
T1705	5681-5684	IN	denotes	for
T1706	5685-5686	DT	denotes	a
T1708	5687-5696	JJ	denotes	secondary
T1707	5697-5703	NN	denotes	signal
T1709	5704-5714	RB	denotes	downstream
T1710	5715-5717	IN	denotes	of
T1711	5718-5721	NN	denotes	SHH
T1712	5722-5724	IN	denotes	in
T1713	5725-5730	JJ	denotes	early
T1714	5731-5741	NN	denotes	patterning
T1715	5741-5742	.	denotes	.
T1716	5742-5956	sentence	denotes	However, all three of these molecules, BMP2, BMP4, and BMP7, have been suggested to act in a second distinct phase of limb patterning, when digit identities are established downstream of earlier patterning events.
T1717	5743-5750	RB	denotes	However
T1719	5750-5752	,	denotes	,
T1720	5752-5755	DT	denotes	all
T1721	5756-5761	CD	denotes	three
T1722	5762-5764	IN	denotes	of
T1723	5765-5770	DT	denotes	these
T1724	5771-5780	NNS	denotes	molecules
T1725	5780-5782	,	denotes	,
T1726	5782-5786	NN	denotes	BMP2
T1727	5786-5788	,	denotes	,
T1728	5788-5792	NN	denotes	BMP4
T1729	5792-5794	,	denotes	,
T1730	5794-5797	CC	denotes	and
T1731	5798-5802	NN	denotes	BMP7
T1732	5802-5804	,	denotes	,
T1733	5804-5808	VBP	denotes	have
T1734	5809-5813	VBN	denotes	been
T1718	5814-5823	VBN	denotes	suggested
T1735	5824-5826	TO	denotes	to
T1736	5827-5830	VB	denotes	act
T1737	5831-5833	IN	denotes	in
T1738	5834-5835	DT	denotes	a
T1740	5836-5842	JJ	denotes	second
T1741	5843-5851	JJ	denotes	distinct
T1739	5852-5857	NN	denotes	phase
T1742	5858-5860	IN	denotes	of
T1743	5861-5865	NN	denotes	limb
T1744	5866-5876	NN	denotes	patterning
T1745	5876-5878	,	denotes	,
T1746	5878-5882	WRB	denotes	when
T1748	5883-5888	NN	denotes	digit
T1749	5889-5899	NNS	denotes	identities
T1750	5900-5903	VBP	denotes	are
T1747	5904-5915	VBN	denotes	established
T1751	5916-5926	RB	denotes	downstream
T1752	5927-5929	IN	denotes	of
T1753	5930-5937	JJR	denotes	earlier
T1755	5938-5948	NN	denotes	patterning
T1754	5949-5955	NNS	denotes	events
T1756	5955-5956	.	denotes	.
T1757	5956-6099	sentence	denotes	In the vertebrate limb, each digit can be uniquely identified based on its size, length, number of phalanges, and location within the autopod.
T1758	5957-5959	IN	denotes	In
T1760	5960-5963	DT	denotes	the
T1762	5964-5974	NN	denotes	vertebrate
T1761	5975-5979	NN	denotes	limb
T1763	5979-5981	,	denotes	,
T1764	5981-5985	DT	denotes	each
T1765	5986-5991	NN	denotes	digit
T1766	5992-5995	MD	denotes	can
T1767	5996-5998	VB	denotes	be
T1768	5999-6007	RB	denotes	uniquely
T1759	6008-6018	VBN	denotes	identified
T1769	6019-6024	VBN	denotes	based
T1770	6025-6027	IN	denotes	on
T1771	6028-6031	PRP$	denotes	its
T1772	6032-6036	NN	denotes	size
T1773	6036-6038	,	denotes	,
T1774	6038-6044	NN	denotes	length
T1775	6044-6046	,	denotes	,
T1776	6046-6052	NN	denotes	number
T1777	6053-6055	IN	denotes	of
T1778	6056-6065	NNS	denotes	phalanges
T1779	6065-6067	,	denotes	,
T1780	6067-6070	CC	denotes	and
T1781	6071-6079	NN	denotes	location
T1782	6080-6086	IN	denotes	within
T1783	6087-6090	DT	denotes	the
T1784	6091-6098	NN	denotes	autopod
T1785	6098-6099	.	denotes	.
T1786	6099-6319	sentence	denotes	As a consequence of the initial establishment of anterior-posterior positioned information within the limb by SHH and/or BMP signaling, the interdigital mesenchyme of the hand plate becomes specified in a graded manner.
T1787	6100-6102	IN	denotes	As
T1789	6103-6104	DT	denotes	a
T1790	6105-6116	NN	denotes	consequence
T1791	6117-6119	IN	denotes	of
T1792	6120-6123	DT	denotes	the
T1794	6124-6131	JJ	denotes	initial
T1793	6132-6145	NN	denotes	establishment
T1795	6146-6148	IN	denotes	of
T1796	6149-6157	JJ	denotes	anterior
T1798	6157-6158	HYPH	denotes	-
T1797	6158-6167	JJ	denotes	posterior
T1799	6168-6178	VBN	denotes	positioned
T1800	6179-6190	NN	denotes	information
T1801	6191-6197	IN	denotes	within
T1802	6198-6201	DT	denotes	the
T1803	6202-6206	NN	denotes	limb
T1804	6207-6209	IN	denotes	by
T1805	6210-6213	NN	denotes	SHH
T1807	6214-6217	CC	denotes	and
T1808	6217-6218	HYPH	denotes	/
T1809	6218-6220	CC	denotes	or
T1810	6221-6224	NN	denotes	BMP
T1806	6225-6234	NN	denotes	signaling
T1811	6234-6236	,	denotes	,
T1812	6236-6239	DT	denotes	the
T1814	6240-6252	JJ	denotes	interdigital
T1813	6253-6263	NN	denotes	mesenchyme
T1815	6264-6266	IN	denotes	of
T1816	6267-6270	DT	denotes	the
T1818	6271-6275	NN	denotes	hand
T1817	6276-6281	NN	denotes	plate
T1788	6282-6289	VBZ	denotes	becomes
T1819	6290-6299	JJ	denotes	specified
T1820	6300-6302	IN	denotes	in
T1821	6303-6304	DT	denotes	a
T1823	6305-6311	VBN	denotes	graded
T1822	6312-6318	NN	denotes	manner
T1824	6318-6319	.	denotes	.
T1825	6319-6450	sentence	denotes	Grafting and extirpation experiments have shown that it is this polarized interdigital tissue which directs digit morphology [15].
T1826	6320-6328	NN	denotes	Grafting
T1828	6329-6332	CC	denotes	and
T1829	6333-6344	NN	denotes	extirpation
T1827	6345-6356	NNS	denotes	experiments
T1831	6357-6361	VBP	denotes	have
T1830	6362-6367	VBN	denotes	shown
T1832	6368-6372	IN	denotes	that
T1834	6373-6375	PRP	denotes	it
T1833	6376-6378	VBZ	denotes	is
T1835	6379-6383	DT	denotes	this
T1837	6384-6393	VBN	denotes	polarized
T1838	6394-6406	JJ	denotes	interdigital
T1836	6407-6413	NN	denotes	tissue
T1839	6414-6419	WDT	denotes	which
T1840	6420-6427	VBZ	denotes	directs
T1841	6428-6433	NN	denotes	digit
T1842	6434-6444	NN	denotes	morphology
T1843	6445-6446	-LRB-	denotes	[
T1844	6446-6448	CD	denotes	15
T1845	6448-6449	-RRB-	denotes	]
T1846	6449-6450	.	denotes	.
T1847	6450-6720	sentence	denotes	BMP2, BMP4, and BMP7 are all expressed in the interdigital mesenchyme [4,14], and experiments inhibiting BMP signaling in this tissue suggested that differential levels of BMPs in the interdigital mesenchyme might be the relevant factor directing digit morphology [15].
T1848	6451-6455	NN	denotes	BMP2
T1850	6455-6457	,	denotes	,
T1851	6457-6461	NN	denotes	BMP4
T1852	6461-6463	,	denotes	,
T1853	6463-6466	CC	denotes	and
T1854	6467-6471	NN	denotes	BMP7
T1855	6472-6475	VBP	denotes	are
T1856	6476-6479	RB	denotes	all
T1849	6480-6489	VBN	denotes	expressed
T1857	6490-6492	IN	denotes	in
T1858	6493-6496	DT	denotes	the
T1860	6497-6509	JJ	denotes	interdigital
T1859	6510-6520	NN	denotes	mesenchyme
T1861	6521-6522	-LRB-	denotes	[
T1863	6522-6523	CD	denotes	4
T1864	6523-6524	,	denotes	,
T1862	6524-6526	CD	denotes	14
T1865	6526-6527	-RRB-	denotes	]
T1866	6527-6529	,	denotes	,
T1867	6529-6532	CC	denotes	and
T1868	6533-6544	NNS	denotes	experiments
T1870	6545-6555	VBG	denotes	inhibiting
T1871	6556-6559	NN	denotes	BMP
T1872	6560-6569	NN	denotes	signaling
T1873	6570-6572	IN	denotes	in
T1874	6573-6577	DT	denotes	this
T1875	6578-6584	NN	denotes	tissue
T1869	6585-6594	VBD	denotes	suggested
T1876	6595-6599	IN	denotes	that
T1878	6600-6612	JJ	denotes	differential
T1879	6613-6619	NNS	denotes	levels
T1880	6620-6622	IN	denotes	of
T1881	6623-6627	NNS	denotes	BMPs
T1882	6628-6630	IN	denotes	in
T1883	6631-6634	DT	denotes	the
T1885	6635-6647	JJ	denotes	interdigital
T1884	6648-6658	NN	denotes	mesenchyme
T1886	6659-6664	MD	denotes	might
T1877	6665-6667	VB	denotes	be
T1887	6668-6671	DT	denotes	the
T1889	6672-6680	JJ	denotes	relevant
T1888	6681-6687	NN	denotes	factor
T1890	6688-6697	VBG	denotes	directing
T1891	6698-6703	NN	denotes	digit
T1892	6704-6714	NN	denotes	morphology
T1893	6715-6716	-LRB-	denotes	[
T1894	6716-6718	CD	denotes	15
T1895	6718-6719	-RRB-	denotes	]
T1896	6719-6720	.	denotes	.
T1897	6720-7023	sentence	denotes	As noted above, BMP7-deficient embryos produce mostly normal limbs, and mice harboring a conditional deletion of Bmp4 in the limb bud mesenchyme also do not show evidence of anterior digit transformation [16], as would have been predicted if BMP4 acted as an interdigital regulator of digit morphology.
T1898	6721-6723	IN	denotes	As
T1899	6724-6729	VBN	denotes	noted
T1901	6730-6735	RB	denotes	above
T1902	6735-6737	,	denotes	,
T1903	6737-6741	NN	denotes	BMP7
T1905	6741-6742	HYPH	denotes	-
T1904	6742-6751	JJ	denotes	deficient
T1906	6752-6759	NNS	denotes	embryos
T1900	6760-6767	VBP	denotes	produce
T1907	6768-6774	RB	denotes	mostly
T1908	6775-6781	JJ	denotes	normal
T1909	6782-6787	NNS	denotes	limbs
T1910	6787-6789	,	denotes	,
T1911	6789-6792	CC	denotes	and
T1912	6793-6797	NNS	denotes	mice
T1914	6798-6807	VBG	denotes	harboring
T1915	6808-6809	DT	denotes	a
T1917	6810-6821	JJ	denotes	conditional
T1916	6822-6830	NN	denotes	deletion
T1918	6831-6833	IN	denotes	of
T1919	6834-6838	NN	denotes	Bmp4
T1920	6839-6841	IN	denotes	in
T1921	6842-6845	DT	denotes	the
T1923	6846-6850	NN	denotes	limb
T1924	6851-6854	NN	denotes	bud
T1922	6855-6865	NN	denotes	mesenchyme
T1925	6866-6870	RB	denotes	also
T1926	6871-6873	VBP	denotes	do
T1927	6874-6877	RB	denotes	not
T1913	6878-6882	VB	denotes	show
T1928	6883-6891	NN	denotes	evidence
T1929	6892-6894	IN	denotes	of
T1930	6895-6903	JJ	denotes	anterior
T1932	6904-6909	NN	denotes	digit
T1931	6910-6924	NN	denotes	transformation
T1933	6925-6926	-LRB-	denotes	[
T1934	6926-6928	CD	denotes	16
T1935	6928-6929	-RRB-	denotes	]
T1936	6929-6931	,	denotes	,
T1937	6931-6933	IN	denotes	as
T1939	6934-6939	MD	denotes	would
T1940	6940-6944	VB	denotes	have
T1941	6945-6949	VBN	denotes	been
T1938	6950-6959	VBN	denotes	predicted
T1942	6960-6962	IN	denotes	if
T1944	6963-6967	NN	denotes	BMP4
T1943	6968-6973	VBD	denotes	acted
T1945	6974-6976	IN	denotes	as
T1946	6977-6979	DT	denotes	an
T1948	6980-6992	JJ	denotes	interdigital
T1947	6993-7002	NN	denotes	regulator
T1949	7003-7005	IN	denotes	of
T1950	7006-7011	NN	denotes	digit
T1951	7012-7022	NN	denotes	morphology
T1952	7022-7023	.	denotes	.
T1953	7023-7283	sentence	denotes	In the light of these observations, it would seem unlikely that quantitative differences in levels of interdigital BMP signal are responsible for establishing digit identity, although redundancy between BMP2, BMP4, and BMP7 in this role remains a possibility.
T1954	7024-7026	IN	denotes	In
T1956	7027-7030	DT	denotes	the
T1957	7031-7036	NN	denotes	light
T1958	7037-7039	IN	denotes	of
T1959	7040-7045	DT	denotes	these
T1960	7046-7058	NNS	denotes	observations
T1961	7058-7060	,	denotes	,
T1962	7060-7062	PRP	denotes	it
T1963	7063-7068	MD	denotes	would
T1955	7069-7073	VB	denotes	seem
T1964	7074-7082	JJ	denotes	unlikely
T1965	7083-7087	IN	denotes	that
T1967	7088-7100	JJ	denotes	quantitative
T1968	7101-7112	NNS	denotes	differences
T1969	7113-7115	IN	denotes	in
T1970	7116-7122	NNS	denotes	levels
T1971	7123-7125	IN	denotes	of
T1972	7126-7138	JJ	denotes	interdigital
T1974	7139-7142	NN	denotes	BMP
T1973	7143-7149	NN	denotes	signal
T1966	7150-7153	VBP	denotes	are
T1975	7154-7165	JJ	denotes	responsible
T1976	7166-7169	IN	denotes	for
T1977	7170-7182	VBG	denotes	establishing
T1978	7183-7188	NN	denotes	digit
T1979	7189-7197	NN	denotes	identity
T1980	7197-7199	,	denotes	,
T1981	7199-7207	IN	denotes	although
T1983	7208-7218	NN	denotes	redundancy
T1984	7219-7226	IN	denotes	between
T1985	7227-7231	NN	denotes	BMP2
T1986	7231-7233	,	denotes	,
T1987	7233-7237	NN	denotes	BMP4
T1988	7237-7239	,	denotes	,
T1989	7239-7242	CC	denotes	and
T1990	7243-7247	NN	denotes	BMP7
T1991	7248-7250	IN	denotes	in
T1992	7251-7255	DT	denotes	this
T1993	7256-7260	NN	denotes	role
T1982	7261-7268	VBZ	denotes	remains
T1994	7269-7270	DT	denotes	a
T1995	7271-7282	NN	denotes	possibility
T1996	7282-7283	.	denotes	.
T1997	7283-7422	sentence	denotes	A third aspect of limb patterning in which BMP2, BMP4, and BMP7 have been implicated is in the apoptotic death of the interdigital tissue.
T1998	7284-7285	DT	denotes	A
T2000	7286-7291	JJ	denotes	third
T1999	7292-7298	NN	denotes	aspect
T2002	7299-7301	IN	denotes	of
T2003	7302-7306	NN	denotes	limb
T2004	7307-7317	NN	denotes	patterning
T2005	7318-7320	IN	denotes	in
T2007	7321-7326	WDT	denotes	which
T2008	7327-7331	NN	denotes	BMP2
T2009	7331-7333	,	denotes	,
T2010	7333-7337	NN	denotes	BMP4
T2011	7337-7339	,	denotes	,
T2012	7339-7342	CC	denotes	and
T2013	7343-7347	NN	denotes	BMP7
T2014	7348-7352	VBP	denotes	have
T2015	7353-7357	VBN	denotes	been
T2006	7358-7368	VBN	denotes	implicated
T2001	7369-7371	VBZ	denotes	is
T2016	7372-7374	IN	denotes	in
T2017	7375-7378	DT	denotes	the
T2019	7379-7388	JJ	denotes	apoptotic
T2018	7389-7394	NN	denotes	death
T2020	7395-7397	IN	denotes	of
T2021	7398-7401	DT	denotes	the
T2023	7402-7414	JJ	denotes	interdigital
T2022	7415-7421	NN	denotes	tissue
T2024	7421-7422	.	denotes	.
T2025	7422-7582	sentence	denotes	For example, ectopic application of BMP antagonists demonstrates that BMP signaling is necessary for this process, and in its absence, webbing occurs [17–19]).
T2026	7423-7426	IN	denotes	For
T2028	7427-7434	NN	denotes	example
T2029	7434-7436	,	denotes	,
T2030	7436-7443	JJ	denotes	ectopic
T2031	7444-7455	NN	denotes	application
T2032	7456-7458	IN	denotes	of
T2033	7459-7462	NN	denotes	BMP
T2034	7463-7474	NNS	denotes	antagonists
T2027	7475-7487	VBZ	denotes	demonstrates
T2035	7488-7492	IN	denotes	that
T2037	7493-7496	NN	denotes	BMP
T2038	7497-7506	NN	denotes	signaling
T2036	7507-7509	VBZ	denotes	is
T2039	7510-7519	JJ	denotes	necessary
T2040	7520-7523	IN	denotes	for
T2041	7524-7528	DT	denotes	this
T2042	7529-7536	NN	denotes	process
T2043	7536-7538	,	denotes	,
T2044	7538-7541	CC	denotes	and
T2045	7542-7544	IN	denotes	in
T2047	7545-7548	PRP$	denotes	its
T2048	7549-7556	NN	denotes	absence
T2049	7556-7558	,	denotes	,
T2050	7558-7565	NN	denotes	webbing
T2046	7566-7572	VBZ	denotes	occurs
T2051	7573-7574	-LRB-	denotes	[
T2052	7574-7576	CD	denotes	17
T2053	7576-7577	SYM	denotes	–
T2054	7577-7579	CD	denotes	19
T2055	7579-7580	-RRB-	denotes	]
T2056	7580-7581	-RRB-	denotes	)
T2057	7581-7582	.	denotes	.
T2058	7582-7817	sentence	denotes	BMP activity also plays an indirect role in limb patterning as a key component of a feedback loop between SHH production in the posterior limb bud and fibroblast growth factor production in the overlying apical ectodermal ridge (AER).
T2059	7583-7586	NN	denotes	BMP
T2060	7587-7595	NN	denotes	activity
T2062	7596-7600	RB	denotes	also
T2061	7601-7606	VBZ	denotes	plays
T2063	7607-7609	DT	denotes	an
T2065	7610-7618	JJ	denotes	indirect
T2064	7619-7623	NN	denotes	role
T2066	7624-7626	IN	denotes	in
T2067	7627-7631	NN	denotes	limb
T2068	7632-7642	NN	denotes	patterning
T2069	7643-7645	IN	denotes	as
T2070	7646-7647	DT	denotes	a
T2072	7648-7651	JJ	denotes	key
T2071	7652-7661	NN	denotes	component
T2073	7662-7664	IN	denotes	of
T2074	7665-7666	DT	denotes	a
T2076	7667-7675	NN	denotes	feedback
T2075	7676-7680	NN	denotes	loop
T2077	7681-7688	IN	denotes	between
T2078	7689-7692	NN	denotes	SHH
T2079	7693-7703	NN	denotes	production
T2080	7704-7706	IN	denotes	in
T2081	7707-7710	DT	denotes	the
T2083	7711-7720	JJ	denotes	posterior
T2084	7721-7725	NN	denotes	limb
T2082	7726-7729	NN	denotes	bud
T2085	7730-7733	CC	denotes	and
T2086	7734-7744	NN	denotes	fibroblast
T2088	7745-7751	NN	denotes	growth
T2089	7752-7758	NN	denotes	factor
T2087	7759-7769	NN	denotes	production
T2090	7770-7772	IN	denotes	in
T2091	7773-7776	DT	denotes	the
T2093	7777-7786	VBG	denotes	overlying
T2094	7787-7793	JJ	denotes	apical
T2095	7794-7804	JJ	denotes	ectodermal
T2092	7805-7810	NN	denotes	ridge
T2096	7811-7812	-LRB-	denotes	(
T2097	7812-7815	NN	denotes	AER
T2098	7815-7816	-RRB-	denotes	)
T2099	7816-7817	.	denotes	.
T2100	7817-7913	sentence	denotes	SHH activity leads to the upregulation of the BMP antagonist Gremlin in the mesenchyme [20,21].
T2101	7818-7821	NN	denotes	SHH
T2102	7822-7830	NN	denotes	activity
T2103	7831-7836	VBZ	denotes	leads
T2104	7837-7839	IN	denotes	to
T2105	7840-7843	DT	denotes	the
T2106	7844-7856	NN	denotes	upregulation
T2107	7857-7859	IN	denotes	of
T2108	7860-7863	DT	denotes	the
T2110	7864-7867	NN	denotes	BMP
T2111	7868-7878	NN	denotes	antagonist
T2109	7879-7886	NN	denotes	Gremlin
T2112	7887-7889	IN	denotes	in
T2113	7890-7893	DT	denotes	the
T2114	7894-7904	NN	denotes	mesenchyme
T2115	7905-7906	-LRB-	denotes	[
T2117	7906-7908	CD	denotes	20
T2118	7908-7909	,	denotes	,
T2116	7909-7911	CD	denotes	21
T2119	7911-7912	-RRB-	denotes	]
T2120	7912-7913	.	denotes	.
T2121	7913-8075	sentence	denotes	This, in turn, prevents BMPs from downregulating fibroblast growth factor (FGF) production in the AER [17,22] and maintains the integrity of the AER itself [23].
T2122	7914-7918	DT	denotes	This
T2124	7918-7920	,	denotes	,
T2125	7920-7922	IN	denotes	in
T2126	7923-7927	NN	denotes	turn
T2127	7927-7929	,	denotes	,
T2123	7929-7937	VBZ	denotes	prevents
T2128	7938-7942	NNS	denotes	BMPs
T2129	7943-7947	IN	denotes	from
T2130	7948-7962	VBG	denotes	downregulating
T2131	7963-7973	NN	denotes	fibroblast
T2133	7974-7980	NN	denotes	growth
T2132	7981-7987	NN	denotes	factor
T2135	7988-7989	-LRB-	denotes	(
T2136	7989-7992	NN	denotes	FGF
T2137	7992-7993	-RRB-	denotes	)
T2134	7994-8004	NN	denotes	production
T2138	8005-8007	IN	denotes	in
T2139	8008-8011	DT	denotes	the
T2140	8012-8015	NN	denotes	AER
T2141	8016-8017	-LRB-	denotes	[
T2143	8017-8019	CD	denotes	17
T2144	8019-8020	,	denotes	,
T2142	8020-8022	CD	denotes	22
T2145	8022-8023	-RRB-	denotes	]
T2146	8024-8027	CC	denotes	and
T2147	8028-8037	VBZ	denotes	maintains
T2148	8038-8041	DT	denotes	the
T2149	8042-8051	NN	denotes	integrity
T2150	8052-8054	IN	denotes	of
T2151	8055-8058	DT	denotes	the
T2152	8059-8062	NN	denotes	AER
T2153	8063-8069	PRP	denotes	itself
T2154	8070-8071	-LRB-	denotes	[
T2155	8071-8073	CD	denotes	23
T2156	8073-8074	-RRB-	denotes	]
T2157	8074-8075	.	denotes	.
T2158	8075-8163	sentence	denotes	FGF signaling feeds back to maintain SHH production in the posterior mesenchyme [8,24].
T2159	8076-8079	NN	denotes	FGF
T2160	8080-8089	NN	denotes	signaling
T2161	8090-8095	VBZ	denotes	feeds
T2162	8096-8100	RB	denotes	back
T2163	8101-8103	TO	denotes	to
T2164	8104-8112	VB	denotes	maintain
T2165	8113-8116	NN	denotes	SHH
T2166	8117-8127	NN	denotes	production
T2167	8128-8130	IN	denotes	in
T2168	8131-8134	DT	denotes	the
T2170	8135-8144	JJ	denotes	posterior
T2169	8145-8155	NN	denotes	mesenchyme
T2171	8156-8157	-LRB-	denotes	[
T2173	8157-8158	CD	denotes	8
T2174	8158-8159	,	denotes	,
T2172	8159-8161	CD	denotes	24
T2175	8161-8162	-RRB-	denotes	]
T2176	8162-8163	.	denotes	.
T2177	8163-8358	sentence	denotes	Thus, conditional removal of BMP4 activity from the posterior limb mesenchyme results in a persistence of the AER, expanded SHH signaling, and consequent preaxial and postaxial polydactyly [16].
T2178	8164-8168	RB	denotes	Thus
T2180	8168-8170	,	denotes	,
T2181	8170-8181	JJ	denotes	conditional
T2182	8182-8189	NN	denotes	removal
T2183	8190-8192	IN	denotes	of
T2184	8193-8197	NN	denotes	BMP4
T2185	8198-8206	NN	denotes	activity
T2186	8207-8211	IN	denotes	from
T2187	8212-8215	DT	denotes	the
T2189	8216-8225	JJ	denotes	posterior
T2190	8226-8230	NN	denotes	limb
T2188	8231-8241	NN	denotes	mesenchyme
T2179	8242-8249	VBZ	denotes	results
T2191	8250-8252	IN	denotes	in
T2192	8253-8254	DT	denotes	a
T2193	8255-8266	NN	denotes	persistence
T2194	8267-8269	IN	denotes	of
T2195	8270-8273	DT	denotes	the
T2196	8274-8277	NN	denotes	AER
T2197	8277-8279	,	denotes	,
T2198	8279-8287	VBN	denotes	expanded
T2200	8288-8291	NN	denotes	SHH
T2199	8292-8301	NN	denotes	signaling
T2201	8301-8303	,	denotes	,
T2202	8303-8306	CC	denotes	and
T2203	8307-8317	JJ	denotes	consequent
T2205	8318-8326	JJ	denotes	preaxial
T2206	8327-8330	CC	denotes	and
T2207	8331-8340	JJ	denotes	postaxial
T2204	8341-8352	NN	denotes	polydactyly
T2208	8353-8354	-LRB-	denotes	[
T2209	8354-8356	CD	denotes	16
T2210	8356-8357	-RRB-	denotes	]
T2211	8357-8358	.	denotes	.
T2212	8358-8467	sentence	denotes	At later stages of limb development, BMP activity is also believed to play critical roles in skeletogenesis.
T2213	8359-8361	IN	denotes	At
T2215	8362-8367	JJ	denotes	later
T2216	8368-8374	NNS	denotes	stages
T2217	8375-8377	IN	denotes	of
T2218	8378-8382	NN	denotes	limb
T2219	8383-8394	NN	denotes	development
T2220	8394-8396	,	denotes	,
T2221	8396-8399	NN	denotes	BMP
T2222	8400-8408	NN	denotes	activity
T2223	8409-8411	VBZ	denotes	is
T2224	8412-8416	RB	denotes	also
T2214	8417-8425	VBN	denotes	believed
T2225	8426-8428	TO	denotes	to
T2226	8429-8433	VB	denotes	play
T2227	8434-8442	JJ	denotes	critical
T2228	8443-8448	NNS	denotes	roles
T2229	8449-8451	IN	denotes	in
T2230	8452-8466	NN	denotes	skeletogenesis
T2231	8466-8467	.	denotes	.
T2232	8467-8650	sentence	denotes	Activation and dominant-negative experiments in the chick have shown that signaling through the BMPR-IB receptor is necessary and sufficient for cartilage condensation in chick [25].
T2233	8468-8478	NN	denotes	Activation
T2235	8479-8482	CC	denotes	and
T2236	8483-8491	JJ	denotes	dominant
T2238	8491-8492	HYPH	denotes	-
T2237	8492-8500	JJ	denotes	negative
T2239	8501-8512	NNS	denotes	experiments
T2240	8513-8515	IN	denotes	in
T2241	8516-8519	DT	denotes	the
T2242	8520-8525	NN	denotes	chick
T2243	8526-8530	VBP	denotes	have
T2234	8531-8536	VBN	denotes	shown
T2244	8537-8541	IN	denotes	that
T2246	8542-8551	VBG	denotes	signaling
T2247	8552-8559	IN	denotes	through
T2248	8560-8563	DT	denotes	the
T2250	8564-8568	NN	denotes	BMPR
T2252	8568-8569	HYPH	denotes	-
T2251	8569-8571	NN	denotes	IB
T2249	8572-8580	NN	denotes	receptor
T2245	8581-8583	VBZ	denotes	is
T2253	8584-8593	JJ	denotes	necessary
T2254	8594-8597	CC	denotes	and
T2255	8598-8608	JJ	denotes	sufficient
T2256	8609-8612	IN	denotes	for
T2257	8613-8622	NN	denotes	cartilage
T2258	8623-8635	NN	denotes	condensation
T2259	8636-8638	IN	denotes	in
T2260	8639-8644	NN	denotes	chick
T2261	8645-8646	-LRB-	denotes	[
T2262	8646-8648	CD	denotes	25
T2263	8648-8649	-RRB-	denotes	]
T2264	8649-8650	.	denotes	.
T2265	8650-8770	sentence	denotes	Likewise, conditional knockout of BmpR1A and BmpR1B blocks all chondrogenic differentiation in the mouse limb bud [26].
T2266	8651-8659	RB	denotes	Likewise
T2268	8659-8661	,	denotes	,
T2269	8661-8672	JJ	denotes	conditional
T2270	8673-8681	NN	denotes	knockout
T2271	8682-8684	IN	denotes	of
T2272	8685-8691	NN	denotes	BmpR1A
T2273	8692-8695	CC	denotes	and
T2274	8696-8702	NN	denotes	BmpR1B
T2267	8703-8709	VBZ	denotes	blocks
T2275	8710-8713	DT	denotes	all
T2277	8714-8726	JJ	denotes	chondrogenic
T2276	8727-8742	NN	denotes	differentiation
T2278	8743-8745	IN	denotes	in
T2279	8746-8749	DT	denotes	the
T2281	8750-8755	NN	denotes	mouse
T2282	8756-8760	NN	denotes	limb
T2280	8761-8764	NN	denotes	bud
T2283	8765-8766	-LRB-	denotes	[
T2284	8766-8768	CD	denotes	26
T2285	8768-8769	-RRB-	denotes	]
T2286	8769-8770	.	denotes	.
T2287	8770-8942	sentence	denotes	Similarly, when noggin, a potent BMP inhibitor, is introduced in early stage chick limb buds prior to skeletogenesis, mesenchymal condensation does not take place [27,28].
T2288	8771-8780	RB	denotes	Similarly
T2290	8780-8782	,	denotes	,
T2291	8782-8786	WRB	denotes	when
T2293	8787-8793	NN	denotes	noggin
T2294	8793-8795	,	denotes	,
T2295	8795-8796	DT	denotes	a
T2297	8797-8803	JJ	denotes	potent
T2298	8804-8807	NN	denotes	BMP
T2296	8808-8817	NN	denotes	inhibitor
T2299	8817-8819	,	denotes	,
T2300	8819-8821	VBZ	denotes	is
T2292	8822-8832	VBN	denotes	introduced
T2301	8833-8835	IN	denotes	in
T2302	8836-8841	JJ	denotes	early
T2303	8842-8847	NN	denotes	stage
T2305	8848-8853	NN	denotes	chick
T2306	8854-8858	NN	denotes	limb
T2304	8859-8863	NNS	denotes	buds
T2307	8864-8869	JJ	denotes	prior
T2308	8870-8872	IN	denotes	to
T2309	8873-8887	NN	denotes	skeletogenesis
T2310	8887-8889	,	denotes	,
T2311	8889-8900	JJ	denotes	mesenchymal
T2312	8901-8913	NN	denotes	condensation
T2313	8914-8918	VBZ	denotes	does
T2314	8919-8922	RB	denotes	not
T2289	8923-8927	VB	denotes	take
T2315	8928-8933	NN	denotes	place
T2316	8934-8935	-LRB-	denotes	[
T2318	8935-8937	CD	denotes	27
T2319	8937-8938	,	denotes	,
T2317	8938-8940	CD	denotes	28
T2320	8940-8941	-RRB-	denotes	]
T2321	8941-8942	.	denotes	.
T2322	8942-9086	sentence	denotes	Taken together, these studies strongly implicate BMP signaling as necessary for mesenchymal condensations and the initiation of chondrogenesis.
T2323	8943-8948	VBN	denotes	Taken
T2325	8949-8957	RB	denotes	together
T2326	8957-8959	,	denotes	,
T2327	8959-8964	DT	denotes	these
T2328	8965-8972	NNS	denotes	studies
T2329	8973-8981	RB	denotes	strongly
T2324	8982-8991	VBP	denotes	implicate
T2330	8992-8995	NN	denotes	BMP
T2331	8996-9005	NN	denotes	signaling
T2332	9006-9008	IN	denotes	as
T2333	9009-9018	JJ	denotes	necessary
T2334	9019-9022	IN	denotes	for
T2335	9023-9034	JJ	denotes	mesenchymal
T2336	9035-9048	NNS	denotes	condensations
T2337	9049-9052	CC	denotes	and
T2338	9053-9056	DT	denotes	the
T2339	9057-9067	NN	denotes	initiation
T2340	9068-9070	IN	denotes	of
T2341	9071-9085	NN	denotes	chondrogenesis
T2342	9085-9086	.	denotes	.
T2343	9086-9271	sentence	denotes	However, the phenotypes in these experiments are severe, causing either global chondrogenesis or absence of chondrogenesis, in the gain- and loss-of-function experiments, respectively.
T2344	9087-9094	RB	denotes	However
T2346	9094-9096	,	denotes	,
T2347	9096-9099	DT	denotes	the
T2348	9100-9110	NNS	denotes	phenotypes
T2349	9111-9113	IN	denotes	in
T2350	9114-9119	DT	denotes	these
T2351	9120-9131	NNS	denotes	experiments
T2345	9132-9135	VBP	denotes	are
T2352	9136-9142	JJ	denotes	severe
T2353	9142-9144	,	denotes	,
T2354	9144-9151	VBG	denotes	causing
T2355	9152-9158	CC	denotes	either
T2357	9159-9165	JJ	denotes	global
T2356	9166-9180	NN	denotes	chondrogenesis
T2358	9181-9183	CC	denotes	or
T2359	9184-9191	NN	denotes	absence
T2360	9192-9194	IN	denotes	of
T2361	9195-9209	NN	denotes	chondrogenesis
T2362	9209-9211	,	denotes	,
T2363	9211-9213	IN	denotes	in
T2364	9214-9217	DT	denotes	the
T2366	9218-9222	NN	denotes	gain
T2367	9222-9223	HYPH	denotes	-
T2368	9224-9227	CC	denotes	and
T2369	9228-9232	NN	denotes	loss
T2370	9232-9233	HYPH	denotes	-
T2371	9233-9235	IN	denotes	of
T2372	9235-9236	HYPH	denotes	-
T2373	9236-9244	NN	denotes	function
T2365	9245-9256	NNS	denotes	experiments
T2374	9256-9258	,	denotes	,
T2375	9258-9270	RB	denotes	respectively
T2376	9270-9271	.	denotes	.
T2377	9271-9476	sentence	denotes	These severe defects during the early stages of skeletogenesis preclude investigation of any specific roles for BMPs in later events in cartilage differentiation, bone formation, and bone metabolism [29].
T2378	9272-9277	DT	denotes	These
T2380	9278-9284	JJ	denotes	severe
T2379	9285-9292	NNS	denotes	defects
T2382	9293-9299	IN	denotes	during
T2383	9300-9303	DT	denotes	the
T2385	9304-9309	JJ	denotes	early
T2384	9310-9316	NNS	denotes	stages
T2386	9317-9319	IN	denotes	of
T2387	9320-9334	NN	denotes	skeletogenesis
T2381	9335-9343	VBP	denotes	preclude
T2388	9344-9357	NN	denotes	investigation
T2389	9358-9360	IN	denotes	of
T2390	9361-9364	DT	denotes	any
T2392	9365-9373	JJ	denotes	specific
T2391	9374-9379	NNS	denotes	roles
T2393	9380-9383	IN	denotes	for
T2394	9384-9388	NNS	denotes	BMPs
T2395	9389-9391	IN	denotes	in
T2396	9392-9397	JJ	denotes	later
T2397	9398-9404	NNS	denotes	events
T2398	9405-9407	IN	denotes	in
T2399	9408-9417	NN	denotes	cartilage
T2400	9418-9433	NN	denotes	differentiation
T2401	9433-9435	,	denotes	,
T2402	9435-9439	NN	denotes	bone
T2403	9440-9449	NN	denotes	formation
T2404	9449-9451	,	denotes	,
T2405	9451-9454	CC	denotes	and
T2406	9455-9459	NN	denotes	bone
T2407	9460-9470	NN	denotes	metabolism
T2408	9471-9472	-LRB-	denotes	[
T2409	9472-9474	CD	denotes	29
T2410	9474-9475	-RRB-	denotes	]
T2411	9475-9476	.	denotes	.
T2412	9476-9648	sentence	denotes	Conversely, removal of individual ligands such as BMP4 [16] or BMP7 [12,13] has not displayed defects in skeletal differentiation, presumably due to functional redundancy.
T2413	9477-9487	RB	denotes	Conversely
T2415	9487-9489	,	denotes	,
T2416	9489-9496	NN	denotes	removal
T2417	9497-9499	IN	denotes	of
T2418	9500-9510	JJ	denotes	individual
T2419	9511-9518	NNS	denotes	ligands
T2420	9519-9523	JJ	denotes	such
T2421	9524-9526	IN	denotes	as
T2422	9527-9531	NN	denotes	BMP4
T2423	9532-9533	-LRB-	denotes	[
T2424	9533-9535	CD	denotes	16
T2425	9535-9536	-RRB-	denotes	]
T2426	9537-9539	CC	denotes	or
T2427	9540-9544	NN	denotes	BMP7
T2428	9545-9546	-LRB-	denotes	[
T2430	9546-9548	CD	denotes	12
T2431	9548-9549	,	denotes	,
T2429	9549-9551	CD	denotes	13
T2432	9551-9552	-RRB-	denotes	]
T2433	9553-9556	VBZ	denotes	has
T2434	9557-9560	RB	denotes	not
T2414	9561-9570	VBN	denotes	displayed
T2435	9571-9578	NNS	denotes	defects
T2436	9579-9581	IN	denotes	in
T2437	9582-9590	JJ	denotes	skeletal
T2438	9591-9606	NN	denotes	differentiation
T2439	9606-9608	,	denotes	,
T2440	9608-9618	RB	denotes	presumably
T2441	9619-9622	IN	denotes	due
T2442	9623-9625	IN	denotes	to
T2443	9626-9636	JJ	denotes	functional
T2444	9637-9647	NN	denotes	redundancy
T2445	9647-9648	.	denotes	.
T2446	9648-9906	sentence	denotes	To gain further insights into the potential roles of BMP signaling in vertebrate limb patterning and skeletogenesis, we have produced mice simultaneously lacking BMP2 and BMP4 activity or BMP2 and BMP7 activity in the limb mesechyme during limb development.
T2447	9649-9651	TO	denotes	To
T2448	9652-9656	VB	denotes	gain
T2450	9657-9664	JJ	denotes	further
T2451	9665-9673	NNS	denotes	insights
T2452	9674-9678	IN	denotes	into
T2453	9679-9682	DT	denotes	the
T2455	9683-9692	JJ	denotes	potential
T2454	9693-9698	NNS	denotes	roles
T2456	9699-9701	IN	denotes	of
T2457	9702-9705	NN	denotes	BMP
T2458	9706-9715	NN	denotes	signaling
T2459	9716-9718	IN	denotes	in
T2460	9719-9729	NN	denotes	vertebrate
T2462	9730-9734	NN	denotes	limb
T2461	9735-9745	NN	denotes	patterning
T2463	9746-9749	CC	denotes	and
T2464	9750-9764	NN	denotes	skeletogenesis
T2465	9764-9766	,	denotes	,
T2466	9766-9768	PRP	denotes	we
T2467	9769-9773	VBP	denotes	have
T2449	9774-9782	VBN	denotes	produced
T2468	9783-9787	NNS	denotes	mice
T2469	9788-9802	RB	denotes	simultaneously
T2470	9803-9810	VBG	denotes	lacking
T2471	9811-9815	NN	denotes	BMP2
T2473	9816-9819	CC	denotes	and
T2474	9820-9824	NN	denotes	BMP4
T2472	9825-9833	NN	denotes	activity
T2475	9834-9836	CC	denotes	or
T2476	9837-9841	NN	denotes	BMP2
T2478	9842-9845	CC	denotes	and
T2479	9846-9850	NN	denotes	BMP7
T2477	9851-9859	NN	denotes	activity
T2480	9860-9862	IN	denotes	in
T2481	9863-9866	DT	denotes	the
T2483	9867-9871	NN	denotes	limb
T2482	9872-9881	NN	denotes	mesechyme
T2484	9882-9888	IN	denotes	during
T2485	9889-9893	NN	denotes	limb
T2486	9894-9905	NN	denotes	development
T2487	9905-9906	.	denotes	.
T2488	9906-10031	sentence	denotes	Since Bmp2 and Bmp4 mutants are both lethal early in embryogenesis [30,31], we used conditional alleles of both these genes.
T2489	9907-9912	IN	denotes	Since
T2491	9913-9917	NN	denotes	Bmp2
T2493	9918-9921	CC	denotes	and
T2494	9922-9926	NN	denotes	Bmp4
T2492	9927-9934	NNS	denotes	mutants
T2490	9935-9938	VBP	denotes	are
T2496	9939-9943	RB	denotes	both
T2497	9944-9950	JJ	denotes	lethal
T2498	9951-9956	JJ	denotes	early
T2499	9957-9959	IN	denotes	in
T2500	9960-9973	NN	denotes	embryogenesis
T2501	9974-9975	-LRB-	denotes	[
T2503	9975-9977	CD	denotes	30
T2504	9977-9978	,	denotes	,
T2502	9978-9980	CD	denotes	31
T2505	9980-9981	-RRB-	denotes	]
T2506	9981-9983	,	denotes	,
T2507	9983-9985	PRP	denotes	we
T2495	9986-9990	VBD	denotes	used
T2508	9991-10002	JJ	denotes	conditional
T2509	10003-10010	NNS	denotes	alleles
T2510	10011-10013	IN	denotes	of
T2511	10014-10018	PDT	denotes	both
T2513	10019-10024	DT	denotes	these
T2512	10025-10030	NNS	denotes	genes
T2514	10030-10031	.	denotes	.
T2515	10031-10199	sentence	denotes	The conditional alleles were deleted early in limb development through the action of a cre-recombinase transgene expressed under the control of the Prx1 enhancer [32].
T2516	10032-10035	DT	denotes	The
T2518	10036-10047	JJ	denotes	conditional
T2517	10048-10055	NNS	denotes	alleles
T2520	10056-10060	VBD	denotes	were
T2519	10061-10068	VBN	denotes	deleted
T2521	10069-10074	RB	denotes	early
T2522	10075-10077	IN	denotes	in
T2523	10078-10082	NN	denotes	limb
T2524	10083-10094	NN	denotes	development
T2525	10095-10102	IN	denotes	through
T2526	10103-10106	DT	denotes	the
T2527	10107-10113	NN	denotes	action
T2528	10114-10116	IN	denotes	of
T2529	10117-10118	DT	denotes	a
T2531	10119-10122	NN	denotes	cre
T2533	10122-10123	HYPH	denotes	-
T2532	10123-10134	NN	denotes	recombinase
T2530	10135-10144	NN	denotes	transgene
T2534	10145-10154	VBN	denotes	expressed
T2535	10155-10160	IN	denotes	under
T2536	10161-10164	DT	denotes	the
T2537	10165-10172	NN	denotes	control
T2538	10173-10175	IN	denotes	of
T2539	10176-10179	DT	denotes	the
T2541	10180-10184	NN	denotes	Prx1
T2540	10185-10193	NN	denotes	enhancer
T2542	10194-10195	-LRB-	denotes	[
T2543	10195-10197	CD	denotes	32
T2544	10197-10198	-RRB-	denotes	]
T2545	10198-10199	.	denotes	.
T2546	10199-10488	sentence	denotes	Neither limbs deficient in BMP2 and BMP7 nor those deficient in BMP2 and BMP4 had defects suggestive of loss of anterior-posterior patterning information, arguing against these factors acting as secondary signals downstream of SHH or acting as interdigital determinants of digit identity.
T2547	10200-10207	CC	denotes	Neither
T2548	10208-10213	NNS	denotes	limbs
T2550	10214-10223	JJ	denotes	deficient
T2551	10224-10226	IN	denotes	in
T2552	10227-10231	NN	denotes	BMP2
T2553	10232-10235	CC	denotes	and
T2554	10236-10240	NN	denotes	BMP7
T2555	10241-10244	CC	denotes	nor
T2556	10245-10250	DT	denotes	those
T2557	10251-10260	JJ	denotes	deficient
T2558	10261-10263	IN	denotes	in
T2559	10264-10268	NN	denotes	BMP2
T2560	10269-10272	CC	denotes	and
T2561	10273-10277	NN	denotes	BMP4
T2549	10278-10281	VBD	denotes	had
T2562	10282-10289	NNS	denotes	defects
T2563	10290-10300	JJ	denotes	suggestive
T2564	10301-10303	IN	denotes	of
T2565	10304-10308	NN	denotes	loss
T2566	10309-10311	IN	denotes	of
T2567	10312-10320	JJ	denotes	anterior
T2569	10320-10321	HYPH	denotes	-
T2568	10321-10330	JJ	denotes	posterior
T2571	10331-10341	NN	denotes	patterning
T2570	10342-10353	NN	denotes	information
T2572	10353-10355	,	denotes	,
T2573	10355-10362	VBG	denotes	arguing
T2574	10363-10370	IN	denotes	against
T2575	10371-10376	DT	denotes	these
T2576	10377-10384	NNS	denotes	factors
T2577	10385-10391	VBG	denotes	acting
T2578	10392-10394	IN	denotes	as
T2579	10395-10404	JJ	denotes	secondary
T2580	10405-10412	NNS	denotes	signals
T2581	10413-10423	RB	denotes	downstream
T2582	10424-10426	IN	denotes	of
T2583	10427-10430	NN	denotes	SHH
T2584	10431-10433	CC	denotes	or
T2585	10434-10440	VBG	denotes	acting
T2586	10441-10443	IN	denotes	as
T2587	10444-10456	JJ	denotes	interdigital
T2588	10457-10469	NNS	denotes	determinants
T2589	10470-10472	IN	denotes	of
T2590	10473-10478	NN	denotes	digit
T2591	10479-10487	NN	denotes	identity
T2592	10487-10488	.	denotes	.
T2593	10488-10734	sentence	denotes	The Bmp2/Bmp4 double mutants did display a loss of posterior digits with a broad posterior hand plate, suggestive of a block in the chondrogenic condensation of the posterior digit rays, rather than a change in anterior-posterior digit identity.
T2594	10489-10492	DT	denotes	The
T2596	10493-10497	NN	denotes	Bmp2
T2598	10497-10498	HYPH	denotes	/
T2597	10498-10502	NN	denotes	Bmp4
T2599	10503-10509	JJ	denotes	double
T2595	10510-10517	NNS	denotes	mutants
T2601	10518-10521	VBD	denotes	did
T2600	10522-10529	VB	denotes	display
T2602	10530-10531	DT	denotes	a
T2603	10532-10536	NN	denotes	loss
T2604	10537-10539	IN	denotes	of
T2605	10540-10549	JJ	denotes	posterior
T2606	10550-10556	NNS	denotes	digits
T2607	10557-10561	IN	denotes	with
T2608	10562-10563	DT	denotes	a
T2610	10564-10569	JJ	denotes	broad
T2611	10570-10579	JJ	denotes	posterior
T2612	10580-10584	NN	denotes	hand
T2609	10585-10590	NN	denotes	plate
T2613	10590-10592	,	denotes	,
T2614	10592-10602	JJ	denotes	suggestive
T2615	10603-10605	IN	denotes	of
T2616	10606-10607	DT	denotes	a
T2617	10608-10613	NN	denotes	block
T2618	10614-10616	IN	denotes	in
T2619	10617-10620	DT	denotes	the
T2621	10621-10633	JJ	denotes	chondrogenic
T2620	10634-10646	NN	denotes	condensation
T2622	10647-10649	IN	denotes	of
T2623	10650-10653	DT	denotes	the
T2625	10654-10663	JJ	denotes	posterior
T2626	10664-10669	NN	denotes	digit
T2624	10670-10674	NNS	denotes	rays
T2627	10674-10676	,	denotes	,
T2628	10676-10682	RB	denotes	rather
T2629	10683-10687	IN	denotes	than
T2630	10688-10689	DT	denotes	a
T2631	10690-10696	NN	denotes	change
T2632	10697-10699	IN	denotes	in
T2633	10700-10708	JJ	denotes	anterior
T2635	10708-10709	HYPH	denotes	-
T2634	10709-10718	JJ	denotes	posterior
T2637	10719-10724	NN	denotes	digit
T2636	10725-10733	NN	denotes	identity
T2638	10733-10734	.	denotes	.
T2639	10734-10851	sentence	denotes	At later stages, the combined activity of neither BMP2 and BMP4 nor of BMP2 and BMP7 is required for chondrogenesis.
T2640	10735-10737	IN	denotes	At
T2642	10738-10743	JJ	denotes	later
T2643	10744-10750	NNS	denotes	stages
T2644	10750-10752	,	denotes	,
T2645	10752-10755	DT	denotes	the
T2647	10756-10764	VBN	denotes	combined
T2646	10765-10773	NN	denotes	activity
T2648	10774-10776	IN	denotes	of
T2649	10777-10784	CC	denotes	neither
T2650	10785-10789	NN	denotes	BMP2
T2651	10790-10793	CC	denotes	and
T2652	10794-10798	NN	denotes	BMP4
T2653	10799-10802	CC	denotes	nor
T2654	10803-10805	IN	denotes	of
T2655	10806-10810	NN	denotes	BMP2
T2656	10811-10814	CC	denotes	and
T2657	10815-10819	NN	denotes	BMP7
T2658	10820-10822	VBZ	denotes	is
T2641	10823-10831	VBN	denotes	required
T2659	10832-10835	IN	denotes	for
T2660	10836-10850	NN	denotes	chondrogenesis
T2661	10850-10851	.	denotes	.
T2662	10851-10933	sentence	denotes	Osteogenesis is also initiated properly in the absence of these pairs of factors.
T2663	10852-10864	NN	denotes	Osteogenesis
T2665	10865-10867	VBZ	denotes	is
T2666	10868-10872	RB	denotes	also
T2664	10873-10882	VBN	denotes	initiated
T2667	10883-10891	RB	denotes	properly
T2668	10892-10894	IN	denotes	in
T2669	10895-10898	DT	denotes	the
T2670	10899-10906	NN	denotes	absence
T2671	10907-10909	IN	denotes	of
T2672	10910-10915	DT	denotes	these
T2673	10916-10921	NNS	denotes	pairs
T2674	10922-10924	IN	denotes	of
T2675	10925-10932	NNS	denotes	factors
T2676	10932-10933	.	denotes	.
T2677	10933-11047	sentence	denotes	However, at later stages, the combined loss of BMP2 and BMP4 evidently becomes limiting, and osteogenesis ceases.
T2678	10934-10941	RB	denotes	However
T2680	10941-10943	,	denotes	,
T2681	10943-10945	IN	denotes	at
T2682	10946-10951	JJ	denotes	later
T2683	10952-10958	NNS	denotes	stages
T2684	10958-10960	,	denotes	,
T2685	10960-10963	DT	denotes	the
T2687	10964-10972	VBN	denotes	combined
T2686	10973-10977	NN	denotes	loss
T2688	10978-10980	IN	denotes	of
T2689	10981-10985	NN	denotes	BMP2
T2690	10986-10989	CC	denotes	and
T2691	10990-10994	NN	denotes	BMP4
T2692	10995-11004	RB	denotes	evidently
T2679	11005-11012	VBZ	denotes	becomes
T2693	11013-11021	JJ	denotes	limiting
T2694	11021-11023	,	denotes	,
T2695	11023-11026	CC	denotes	and
T2696	11027-11039	NN	denotes	osteogenesis
T2697	11040-11046	VBZ	denotes	ceases
T2698	11046-11047	.	denotes	.
T2699	11047-11327	sentence	denotes	As all prior reported manipulations of the BMP pathway during skeletal development either result in a block at the first stage of chondrogenesis or allow complete skeletal formation, this is the first demonstration of a requirement for BMP activity in osteogenic differentiation.
T2700	11048-11050	IN	denotes	As
T2702	11051-11054	DT	denotes	all
T2704	11055-11060	RB	denotes	prior
T2705	11061-11069	VBN	denotes	reported
T2703	11070-11083	NNS	denotes	manipulations
T2706	11084-11086	IN	denotes	of
T2707	11087-11090	DT	denotes	the
T2709	11091-11094	NN	denotes	BMP
T2708	11095-11102	NN	denotes	pathway
T2710	11103-11109	IN	denotes	during
T2711	11110-11118	JJ	denotes	skeletal
T2712	11119-11130	NN	denotes	development
T2713	11131-11137	CC	denotes	either
T2701	11138-11144	VBP	denotes	result
T2715	11145-11147	IN	denotes	in
T2716	11148-11149	DT	denotes	a
T2717	11150-11155	NN	denotes	block
T2718	11156-11158	IN	denotes	at
T2719	11159-11162	DT	denotes	the
T2721	11163-11168	JJ	denotes	first
T2720	11169-11174	NN	denotes	stage
T2722	11175-11177	IN	denotes	of
T2723	11178-11192	NN	denotes	chondrogenesis
T2724	11193-11195	CC	denotes	or
T2725	11196-11201	VB	denotes	allow
T2726	11202-11210	JJ	denotes	complete
T2728	11211-11219	JJ	denotes	skeletal
T2727	11220-11229	NN	denotes	formation
T2729	11229-11231	,	denotes	,
T2730	11231-11235	DT	denotes	this
T2714	11236-11238	VBZ	denotes	is
T2731	11239-11242	DT	denotes	the
T2733	11243-11248	JJ	denotes	first
T2732	11249-11262	NN	denotes	demonstration
T2734	11263-11265	IN	denotes	of
T2735	11266-11267	DT	denotes	a
T2736	11268-11279	NN	denotes	requirement
T2737	11280-11283	IN	denotes	for
T2738	11284-11287	NN	denotes	BMP
T2739	11288-11296	NN	denotes	activity
T2740	11297-11299	IN	denotes	in
T2741	11300-11310	JJ	denotes	osteogenic
T2742	11311-11326	NN	denotes	differentiation
T2743	11326-11327	.	denotes	.
T2948	11337-11339	TO	denotes	To
T2949	11340-11351	VB	denotes	investigate
T2951	11352-11355	DT	denotes	the
T2952	11356-11361	NNS	denotes	roles
T2953	11362-11364	IN	denotes	of
T2954	11365-11368	NN	denotes	BMP
T2955	11369-11378	NN	denotes	signaling
T2956	11379-11381	IN	denotes	at
T2957	11382-11389	JJ	denotes	various
T2958	11390-11396	NNS	denotes	stages
T2959	11397-11399	IN	denotes	of
T2960	11400-11404	NN	denotes	limb
T2961	11405-11415	NN	denotes	patterning
T2962	11416-11419	CC	denotes	and
T2963	11420-11434	NN	denotes	skeletogenesis
T2964	11434-11436	,	denotes	,
T2965	11436-11438	PRP	denotes	we
T2950	11439-11450	VBD	denotes	constructed
T2966	11451-11452	DT	denotes	a
T2967	11453-11459	NN	denotes	series
T2968	11460-11462	IN	denotes	of
T2969	11463-11467	NNS	denotes	mice
T2970	11468-11477	JJ	denotes	deficient
T2971	11478-11484	RB	denotes	singly
T2973	11485-11487	CC	denotes	or
T2972	11488-11490	IN	denotes	in
T2974	11491-11502	NN	denotes	combination
T2975	11503-11505	IN	denotes	in
T2976	11506-11509	DT	denotes	the
T2977	11510-11517	NN	denotes	ability
T2978	11518-11520	TO	denotes	to
T2979	11521-11528	VB	denotes	produce
T2980	11529-11533	NN	denotes	BMP2
T2981	11533-11535	,	denotes	,
T2982	11535-11539	NN	denotes	BMP4
T2983	11539-11541	,	denotes	,
T2984	11541-11544	CC	denotes	and
T2985	11545-11549	NN	denotes	BMP7
T2986	11549-11550	.	denotes	.
T2987	11550-11630	sentence	denotes	BMP7-deficient mice (kindly provided by Dr. Liz Robertson) survive until birth.
T2988	11551-11555	NN	denotes	BMP7
T2990	11555-11556	HYPH	denotes	-
T2989	11556-11565	JJ	denotes	deficient
T2991	11566-11570	NNS	denotes	mice
T2993	11571-11572	-LRB-	denotes	(
T2994	11572-11578	RB	denotes	kindly
T2995	11579-11587	VBN	denotes	provided
T2996	11588-11590	IN	denotes	by
T2997	11591-11594	NNP	denotes	Dr.
T2999	11595-11598	NNP	denotes	Liz
T2998	11599-11608	NNP	denotes	Robertson
T3000	11608-11609	-RRB-	denotes	)
T2992	11610-11617	VBP	denotes	survive
T3001	11618-11623	IN	denotes	until
T3002	11624-11629	NN	denotes	birth
T3003	11629-11630	.	denotes	.
T3004	11630-11725	sentence	denotes	However, BMP2 and BMP4 are both required for viability early in embryonic development [30,31].
T3005	11631-11638	RB	denotes	However
T3007	11638-11640	,	denotes	,
T3008	11640-11644	NN	denotes	BMP2
T3009	11645-11648	CC	denotes	and
T3010	11649-11653	NN	denotes	BMP4
T3011	11654-11657	VBP	denotes	are
T3012	11658-11662	RB	denotes	both
T3006	11663-11671	VBN	denotes	required
T3013	11672-11675	IN	denotes	for
T3014	11676-11685	NN	denotes	viability
T3015	11686-11691	RB	denotes	early
T3016	11692-11694	IN	denotes	in
T3017	11695-11704	JJ	denotes	embryonic
T3018	11705-11716	NN	denotes	development
T3019	11717-11718	-LRB-	denotes	[
T3021	11718-11720	CD	denotes	30
T3022	11720-11721	,	denotes	,
T3020	11721-11723	CD	denotes	31
T3023	11723-11724	-RRB-	denotes	]
T3024	11724-11725	.	denotes	.
T3025	11725-11820	sentence	denotes	We therefore constructed a conditional allele of Bmp2, introducing loxP sites flanking exon 3.
T3026	11726-11728	PRP	denotes	We
T3028	11729-11738	RB	denotes	therefore
T3027	11739-11750	VBD	denotes	constructed
T3029	11751-11752	DT	denotes	a
T3031	11753-11764	JJ	denotes	conditional
T3030	11765-11771	NN	denotes	allele
T3032	11772-11774	IN	denotes	of
T3033	11775-11779	NN	denotes	Bmp2
T3034	11779-11781	,	denotes	,
T3035	11781-11792	VBG	denotes	introducing
T3036	11793-11797	NN	denotes	loxP
T3037	11798-11803	NNS	denotes	sites
T3038	11804-11812	VBG	denotes	flanking
T3039	11813-11817	NN	denotes	exon
T3040	11818-11819	CD	denotes	3
T3041	11819-11820	.	denotes	.
T3042	11820-11955	sentence	denotes	We obtained a conditional allele of Bmp4, in which exon 4 is flanked by loxP sites, from Dr. Holger Kulessa and Dr. Brigid Hogan [16].
T3043	11821-11823	PRP	denotes	We
T3044	11824-11832	VBD	denotes	obtained
T3045	11833-11834	DT	denotes	a
T3047	11835-11846	JJ	denotes	conditional
T3046	11847-11853	NN	denotes	allele
T3048	11854-11856	IN	denotes	of
T3049	11857-11861	NN	denotes	Bmp4
T3050	11861-11863	,	denotes	,
T3051	11863-11865	IN	denotes	in
T3053	11866-11871	WDT	denotes	which
T3054	11872-11876	NN	denotes	exon
T3055	11877-11878	CD	denotes	4
T3056	11879-11881	VBZ	denotes	is
T3052	11882-11889	VBN	denotes	flanked
T3057	11890-11892	IN	denotes	by
T3058	11893-11897	NN	denotes	loxP
T3059	11898-11903	NNS	denotes	sites
T3060	11903-11905	,	denotes	,
T3061	11905-11909	IN	denotes	from
T3062	11910-11913	NNP	denotes	Dr.
T3064	11914-11920	NNP	denotes	Holger
T3063	11921-11928	NNP	denotes	Kulessa
T3065	11929-11932	CC	denotes	and
T3066	11933-11936	NNP	denotes	Dr.
T3068	11937-11943	NNP	denotes	Brigid
T3067	11944-11949	NNP	denotes	Hogan
T3069	11950-11951	-LRB-	denotes	[
T3070	11951-11953	CD	denotes	16
T3071	11953-11954	-RRB-	denotes	]
T3072	11954-11955	.	denotes	.
T3073	11955-12086	sentence	denotes	Both of these alleles would be expected to result in null alleles following recombination (see Materials and Methods for details).
T3074	11956-11960	DT	denotes	Both
T3076	11961-11963	IN	denotes	of
T3077	11964-11969	DT	denotes	these
T3078	11970-11977	NNS	denotes	alleles
T3079	11978-11983	MD	denotes	would
T3080	11984-11986	VB	denotes	be
T3075	11987-11995	VBN	denotes	expected
T3081	11996-11998	TO	denotes	to
T3082	11999-12005	VB	denotes	result
T3083	12006-12008	IN	denotes	in
T3084	12009-12013	JJ	denotes	null
T3085	12014-12021	NNS	denotes	alleles
T3086	12022-12031	VBG	denotes	following
T3087	12032-12045	NN	denotes	recombination
T3088	12046-12047	-LRB-	denotes	(
T3089	12047-12050	VB	denotes	see
T3090	12051-12060	NNS	denotes	Materials
T3091	12061-12064	CC	denotes	and
T3092	12065-12072	NNS	denotes	Methods
T3093	12073-12076	IN	denotes	for
T3094	12077-12084	NNS	denotes	details
T3095	12084-12085	-RRB-	denotes	)
T3096	12085-12086	.	denotes	.
T3097	12086-12268	sentence	denotes	To conditionally inactivate Bmp2 and Bmp4 in the limb, we used a well-characterized transgene in which cre-recombinase is expressed under the control of the Prx1 limb enhancer [32].
T3098	12087-12089	TO	denotes	To
T3100	12090-12103	RB	denotes	conditionally
T3099	12104-12114	VB	denotes	inactivate
T3102	12115-12119	NN	denotes	Bmp2
T3103	12120-12123	CC	denotes	and
T3104	12124-12128	NN	denotes	Bmp4
T3105	12129-12131	IN	denotes	in
T3106	12132-12135	DT	denotes	the
T3107	12136-12140	NN	denotes	limb
T3108	12140-12142	,	denotes	,
T3109	12142-12144	PRP	denotes	we
T3101	12145-12149	VBD	denotes	used
T3110	12150-12151	DT	denotes	a
T3112	12152-12156	RB	denotes	well
T3114	12156-12157	HYPH	denotes	-
T3113	12157-12170	VBN	denotes	characterized
T3111	12171-12180	NN	denotes	transgene
T3115	12181-12183	IN	denotes	in
T3117	12184-12189	WDT	denotes	which
T3118	12190-12193	NN	denotes	cre
T3120	12193-12194	HYPH	denotes	-
T3119	12194-12205	NN	denotes	recombinase
T3121	12206-12208	VBZ	denotes	is
T3116	12209-12218	VBN	denotes	expressed
T3122	12219-12224	IN	denotes	under
T3123	12225-12228	DT	denotes	the
T3124	12229-12236	NN	denotes	control
T3125	12237-12239	IN	denotes	of
T3126	12240-12243	DT	denotes	the
T3128	12244-12248	NN	denotes	Prx1
T3129	12249-12253	NN	denotes	limb
T3127	12254-12262	NN	denotes	enhancer
T3130	12263-12264	-LRB-	denotes	[
T3131	12264-12266	CD	denotes	32
T3132	12266-12267	-RRB-	denotes	]
T3133	12267-12268	.	denotes	.
T3134	12268-12409	sentence	denotes	This transgene expresses cre very early in limb development, resulting in complete recombination of floxed alleles at early limb bud stages.
T3135	12269-12273	DT	denotes	This
T3136	12274-12283	NN	denotes	transgene
T3137	12284-12293	VBZ	denotes	expresses
T3138	12294-12297	NN	denotes	cre
T3139	12298-12302	RB	denotes	very
T3140	12303-12308	RB	denotes	early
T3141	12309-12311	IN	denotes	in
T3142	12312-12316	NN	denotes	limb
T3143	12317-12328	NN	denotes	development
T3144	12328-12330	,	denotes	,
T3145	12330-12339	VBG	denotes	resulting
T3146	12340-12342	IN	denotes	in
T3147	12343-12351	JJ	denotes	complete
T3148	12352-12365	NN	denotes	recombination
T3149	12366-12368	IN	denotes	of
T3150	12369-12375	VBN	denotes	floxed
T3151	12376-12383	NNS	denotes	alleles
T3152	12384-12386	IN	denotes	at
T3153	12387-12392	JJ	denotes	early
T3155	12393-12397	NN	denotes	limb
T3156	12398-12401	NN	denotes	bud
T3154	12402-12408	NNS	denotes	stages
T3157	12408-12409	.	denotes	.
T3158	12409-12554	sentence	denotes	We verified the ability of Prx1::cre to recombine the conditional Bmp2 and Bmp4 alleles at earliest limb bud stages using in situ hybridization.
T3159	12410-12412	PRP	denotes	We
T3160	12413-12421	VBD	denotes	verified
T3161	12422-12425	DT	denotes	the
T3162	12426-12433	NN	denotes	ability
T3163	12434-12436	IN	denotes	of
T3164	12437-12441	NN	denotes	Prx1
T3166	12441-12442	HYPH	denotes	:
T3167	12442-12443	HYPH	denotes	:
T3165	12443-12446	NN	denotes	cre
T3168	12447-12449	TO	denotes	to
T3169	12450-12459	VB	denotes	recombine
T3170	12460-12463	DT	denotes	the
T3172	12464-12475	JJ	denotes	conditional
T3173	12476-12480	NN	denotes	Bmp2
T3174	12481-12484	CC	denotes	and
T3175	12485-12489	NN	denotes	Bmp4
T3171	12490-12497	NNS	denotes	alleles
T3176	12498-12500	IN	denotes	at
T3177	12501-12509	JJS	denotes	earliest
T3179	12510-12514	NN	denotes	limb
T3180	12515-12518	NN	denotes	bud
T3178	12519-12525	NNS	denotes	stages
T3181	12526-12531	VBG	denotes	using
T3182	12532-12534	FW	denotes	in
T3183	12535-12539	FW	denotes	situ
T3184	12540-12553	NN	denotes	hybridization
T3185	12553-12554	.	denotes	.
T3187	12555-12559	NN	denotes	Bmp2
T3186	12555-12662	sentence	denotes	Bmp2 is first expressed in the limb mesenchyme at embryonic day (E)10.5 in the mouse (Figure 1A, asterisk).
T3189	12560-12562	VBZ	denotes	is
T3190	12563-12568	RB	denotes	first
T3188	12569-12578	VBN	denotes	expressed
T3191	12579-12581	IN	denotes	in
T3192	12582-12585	DT	denotes	the
T3194	12586-12590	NN	denotes	limb
T3193	12591-12601	NN	denotes	mesenchyme
T3195	12602-12604	IN	denotes	at
T3196	12605-12614	JJ	denotes	embryonic
T3197	12615-12618	NN	denotes	day
T3198	12619-12620	-LRB-	denotes	(
T3199	12620-12621	NN	denotes	E
T3200	12621-12622	-RRB-	denotes	)
T3201	12622-12626	CD	denotes	10.5
T3202	12627-12629	IN	denotes	in
T3203	12630-12633	DT	denotes	the
T3204	12634-12639	NN	denotes	mouse
T3205	12640-12641	-LRB-	denotes	(
T3207	12641-12647	NN	denotes	Figure
T3208	12648-12650	NN	denotes	1A
T3209	12650-12652	,	denotes	,
T3206	12652-12660	NN	denotes	asterisk
T3210	12660-12661	-RRB-	denotes	)
T3211	12661-12662	.	denotes	.
T3212	12662-12894	sentence	denotes	By the time its expression is first detectable, the floxed Bmp2 allele appears to be completely recombined in the limb mesenchyme, as whole mount in situ hybridization does not detect any mesenchymal Bmp2 transcription (Figure 1B).
T3213	12663-12665	IN	denotes	By
T3215	12666-12669	DT	denotes	the
T3216	12670-12674	NN	denotes	time
T3217	12675-12678	PRP$	denotes	its
T3218	12679-12689	NN	denotes	expression
T3219	12690-12692	VBZ	denotes	is
T3220	12693-12698	RB	denotes	first
T3221	12699-12709	JJ	denotes	detectable
T3222	12709-12711	,	denotes	,
T3223	12711-12714	DT	denotes	the
T3225	12715-12721	VBN	denotes	floxed
T3226	12722-12726	NN	denotes	Bmp2
T3224	12727-12733	NN	denotes	allele
T3214	12734-12741	VBZ	denotes	appears
T3227	12742-12744	TO	denotes	to
T3229	12745-12747	VB	denotes	be
T3230	12748-12758	RB	denotes	completely
T3228	12759-12769	VBN	denotes	recombined
T3231	12770-12772	IN	denotes	in
T3232	12773-12776	DT	denotes	the
T3234	12777-12781	NN	denotes	limb
T3233	12782-12792	NN	denotes	mesenchyme
T3235	12792-12794	,	denotes	,
T3236	12794-12796	IN	denotes	as
T3238	12797-12802	JJ	denotes	whole
T3239	12803-12808	NN	denotes	mount
T3241	12809-12811	FW	denotes	in
T3242	12812-12816	FW	denotes	situ
T3240	12817-12830	NN	denotes	hybridization
T3243	12831-12835	VBZ	denotes	does
T3244	12836-12839	RB	denotes	not
T3237	12840-12846	VB	denotes	detect
T3245	12847-12850	DT	denotes	any
T3247	12851-12862	JJ	denotes	mesenchymal
T3248	12863-12867	NN	denotes	Bmp2
T3246	12868-12881	NN	denotes	transcription
T3249	12882-12883	-LRB-	denotes	(
T3251	12883-12889	NN	denotes	Figure
T3250	12890-12892	NN	denotes	1B
T3252	12892-12893	-RRB-	denotes	)
T3253	12893-12894	.	denotes	.
T3254	12894-13028	sentence	denotes	At E10.5 Bmp4 is expressed in the mouse limb mesenchyme in two stripes at the anterior and posterior margins (Figure 1C, red arrows).
T3255	12895-12897	IN	denotes	At
T3257	12898-12903	NN	denotes	E10.5
T3258	12904-12908	NN	denotes	Bmp4
T3259	12909-12911	VBZ	denotes	is
T3256	12912-12921	VBN	denotes	expressed
T3260	12922-12924	IN	denotes	in
T3261	12925-12928	DT	denotes	the
T3263	12929-12934	NN	denotes	mouse
T3264	12935-12939	NN	denotes	limb
T3262	12940-12950	NN	denotes	mesenchyme
T3265	12951-12953	IN	denotes	in
T3266	12954-12957	CD	denotes	two
T3267	12958-12965	NNS	denotes	stripes
T3268	12966-12968	IN	denotes	at
T3269	12969-12972	DT	denotes	the
T3271	12973-12981	JJ	denotes	anterior
T3272	12982-12985	CC	denotes	and
T3273	12986-12995	JJ	denotes	posterior
T3270	12996-13003	NNS	denotes	margins
T3274	13004-13005	-LRB-	denotes	(
T3276	13005-13011	NN	denotes	Figure
T3277	13012-13014	NN	denotes	1C
T3278	13014-13016	,	denotes	,
T3279	13016-13019	JJ	denotes	red
T3275	13020-13026	NNS	denotes	arrows
T3280	13026-13027	-RRB-	denotes	)
T3281	13027-13028	.	denotes	.
T3282	13028-13138	sentence	denotes	These expression domains are completely lost by E10.5 in the presence of the Prx1::cre transgene (Figure 1D).
T3283	13029-13034	DT	denotes	These
T3285	13035-13045	NN	denotes	expression
T3284	13046-13053	NNS	denotes	domains
T3287	13054-13057	VBP	denotes	are
T3288	13058-13068	RB	denotes	completely
T3286	13069-13073	VBN	denotes	lost
T3289	13074-13076	IN	denotes	by
T3290	13077-13082	NN	denotes	E10.5
T3291	13083-13085	IN	denotes	in
T3292	13086-13089	DT	denotes	the
T3293	13090-13098	NN	denotes	presence
T3294	13099-13101	IN	denotes	of
T3295	13102-13105	DT	denotes	the
T3297	13106-13110	NN	denotes	Prx1
T3299	13110-13111	HYPH	denotes	:
T3300	13111-13112	HYPH	denotes	:
T3298	13112-13115	NN	denotes	cre
T3296	13116-13125	NN	denotes	transgene
T3301	13126-13127	-LRB-	denotes	(
T3303	13127-13133	NN	denotes	Figure
T3302	13134-13136	NN	denotes	1D
T3304	13136-13137	-RRB-	denotes	)
T3305	13137-13138	.	denotes	.
T3307	13139-13143	NN	denotes	Bmp2
T3306	13139-13291	sentence	denotes	Bmp2 and Bmp4 are also expressed in the AER, where Prx1::cre is inactive, and these domains of expression are not affected (Figure 1A–1D, black arrows).
T3309	13144-13147	CC	denotes	and
T3310	13148-13152	NN	denotes	Bmp4
T3311	13153-13156	VBP	denotes	are
T3312	13157-13161	RB	denotes	also
T3308	13162-13171	VBN	denotes	expressed
T3313	13172-13174	IN	denotes	in
T3314	13175-13178	DT	denotes	the
T3315	13179-13182	NN	denotes	AER
T3316	13182-13184	,	denotes	,
T3317	13184-13189	WRB	denotes	where
T3319	13190-13194	NN	denotes	Prx1
T3321	13194-13195	HYPH	denotes	:
T3322	13195-13196	HYPH	denotes	:
T3320	13196-13199	NN	denotes	cre
T3318	13200-13202	VBZ	denotes	is
T3323	13203-13211	JJ	denotes	inactive
T3324	13211-13213	,	denotes	,
T3325	13213-13216	CC	denotes	and
T3326	13217-13222	DT	denotes	these
T3327	13223-13230	NNS	denotes	domains
T3329	13231-13233	IN	denotes	of
T3330	13234-13244	NN	denotes	expression
T3331	13245-13248	VBP	denotes	are
T3332	13249-13252	RB	denotes	not
T3328	13253-13261	VBN	denotes	affected
T3333	13262-13263	-LRB-	denotes	(
T3335	13263-13269	NN	denotes	Figure
T3336	13270-13272	NN	denotes	1A
T3337	13272-13273	SYM	denotes	–
T3338	13273-13275	NN	denotes	1D
T3339	13275-13277	,	denotes	,
T3340	13277-13282	JJ	denotes	black
T3334	13283-13289	NNS	denotes	arrows
T3341	13289-13290	-RRB-	denotes	)
T3342	13290-13291	.	denotes	.
T3720	13302-13304	DT	denotes	An
T3722	13305-13312	JJ	denotes	Allelic
T3724	13312-13313	HYPH	denotes	/
T3723	13313-13323	JJ	denotes	Nonallelic
T3721	13324-13330	NN	denotes	Series
T3725	13331-13333	IN	denotes	of
T3726	13334-13337	NN	denotes	BMP
T3728	13337-13338	HYPH	denotes	-
T3727	13338-13347	JJ	denotes	Deficient
T3729	13348-13353	NNS	denotes	Limbs
T3730	13353-14912	sentence	denotes	(A–D) Prx1::cre efficiently recombines Bmp2 and Bmp4 conditional alleles in the limbs. Bmp2 (A and B) and Bmp4 (C and D) whole mount mRNA in situ hybridization in the limb. Wild-type (A) and Bmp2C/C; Prx1::cre (B) are forelimbs from E10.5 mouse embryos. Mesenchymal expression [asterisk (A)] of Bmp2 is abolished in Bmp2C/C; Prx1::cre embryo while the AER expression [black arrow (A and B)] of Bmp2 persists. Note that pink staining in the central region of the limb bud in (B) is nonspecific background. Wild-type (C) and Bmp4C/C; Prx1::cre (D) are forelimbs from E10.5 mouse embryos. Mesenchymal expression [red arrow (C)] of Bmp4 is abolished in Bmp4C/C; Prx1::cre embryo while the AER expression [black arrow (C and D)] of Bmp4 persists. (E–T) Depletion of BMP2 and BMP4 together causes severe limb skeletal defects. (E–T) Whole mount skeletons from newborn animals stained with Alcian blue and Alizarin red. (E–L) Forelimbs, (M–T) hindlimbs. (E and M) Wild-type, (F and N) Bmp2C/C; Prx1::cre, (G and O) Bmp4C/C; Prx1::cre, (H and P) Bmp7 −/−, (I and Q) Bmp2C/C; Bmp4+/C; Prx1::cre, (J and R) Bmp2+/C; Bmp4C/C; Prx1::cre, (K and S) Bmp2C/C; Bmp4C/C; Prx1::cre, (L and T) Bmp2C/C, Bmp7 −/−; Prx1::cre. Thin red arrow in (F), (I), and (L), defective scapula; thick red arrow in (T), failure of fibula to articulate with knee, and thick black arrow in (L) and (T), missing phalanx in digit III. An Allelic/Nonallelic Series of BMP-Deficient Limbs Mice were generated with limbs deficient in BMP2, BMP4, or BMP7, both BMP2 and BMP4, or both BMP2 and BMP7.
T3731	14805-14809	NNS	denotes	Mice
T3733	14810-14814	VBD	denotes	were
T3732	14815-14824	VBN	denotes	generated
T3734	14825-14829	IN	denotes	with
T3735	14830-14835	NNS	denotes	limbs
T3736	14836-14845	JJ	denotes	deficient
T3737	14846-14848	IN	denotes	in
T3738	14849-14853	NN	denotes	BMP2
T3739	14853-14855	,	denotes	,
T3740	14855-14859	NN	denotes	BMP4
T3741	14859-14861	,	denotes	,
T3742	14861-14863	CC	denotes	or
T3743	14864-14868	NN	denotes	BMP7
T3744	14868-14870	,	denotes	,
T3745	14870-14874	CC	denotes	both
T3746	14875-14879	NN	denotes	BMP2
T3747	14880-14883	CC	denotes	and
T3748	14884-14888	NN	denotes	BMP4
T3749	14888-14890	,	denotes	,
T3750	14890-14892	CC	denotes	or
T3751	14893-14897	CC	denotes	both
T3752	14898-14902	NN	denotes	BMP2
T3753	14903-14906	CC	denotes	and
T3754	14907-14911	NN	denotes	BMP7
T3755	14911-14912	.	denotes	.
T3756	14912-15055	sentence	denotes	To obtain an initial indication of the range of phenotypes produced in these animals, we first examined the limb skeletons of newborn animals.
T3757	14913-14915	TO	denotes	To
T3758	14916-14922	VB	denotes	obtain
T3760	14923-14925	DT	denotes	an
T3762	14926-14933	JJ	denotes	initial
T3761	14934-14944	NN	denotes	indication
T3763	14945-14947	IN	denotes	of
T3764	14948-14951	DT	denotes	the
T3765	14952-14957	NN	denotes	range
T3766	14958-14960	IN	denotes	of
T3767	14961-14971	NNS	denotes	phenotypes
T3768	14972-14980	VBN	denotes	produced
T3769	14981-14983	IN	denotes	in
T3770	14984-14989	DT	denotes	these
T3771	14990-14997	NNS	denotes	animals
T3772	14997-14999	,	denotes	,
T3773	14999-15001	PRP	denotes	we
T3774	15002-15007	RB	denotes	first
T3759	15008-15016	VBD	denotes	examined
T3775	15017-15020	DT	denotes	the
T3777	15021-15025	NN	denotes	limb
T3776	15026-15035	NNS	denotes	skeletons
T3778	15036-15038	IN	denotes	of
T3779	15039-15046	JJ	denotes	newborn
T3780	15047-15054	NNS	denotes	animals
T3781	15054-15055	.	denotes	.
T3782	15055-15233	sentence	denotes	BMP2-deficient limbs appeared remarkably normal, both in skeletal pattern and in gross aspects of chondrogenesis and osteogenesis indicated by Alcian blue/Alizarin red staining.
T3783	15056-15060	NN	denotes	BMP2
T3785	15060-15061	HYPH	denotes	-
T3784	15061-15070	JJ	denotes	deficient
T3786	15071-15076	NNS	denotes	limbs
T3787	15077-15085	VBD	denotes	appeared
T3788	15086-15096	RB	denotes	remarkably
T3789	15097-15103	JJ	denotes	normal
T3790	15103-15105	,	denotes	,
T3791	15105-15109	CC	denotes	both
T3792	15110-15112	IN	denotes	in
T3793	15113-15121	JJ	denotes	skeletal
T3794	15122-15129	NN	denotes	pattern
T3795	15130-15133	CC	denotes	and
T3796	15134-15136	IN	denotes	in
T3797	15137-15142	JJ	denotes	gross
T3798	15143-15150	NNS	denotes	aspects
T3799	15151-15153	IN	denotes	of
T3800	15154-15168	NN	denotes	chondrogenesis
T3801	15169-15172	CC	denotes	and
T3802	15173-15185	NN	denotes	osteogenesis
T3803	15186-15195	VBN	denotes	indicated
T3804	15196-15198	IN	denotes	by
T3805	15199-15205	JJ	denotes	Alcian
T3806	15206-15210	JJ	denotes	blue
T3808	15210-15211	HYPH	denotes	/
T3809	15211-15219	JJ	denotes	Alizarin
T3807	15220-15223	JJ	denotes	red
T3810	15224-15232	NN	denotes	staining
T3811	15232-15233	.	denotes	.
T3812	15233-15373	sentence	denotes	The one notable defect in the appendicular skeleton of these animals is a characteristic malformation of the scapula (Figure 1, red arrow).
T3813	15234-15237	DT	denotes	The
T3815	15238-15241	CD	denotes	one
T3816	15242-15249	JJ	denotes	notable
T3814	15250-15256	NN	denotes	defect
T3818	15257-15259	IN	denotes	in
T3819	15260-15263	DT	denotes	the
T3821	15264-15276	JJ	denotes	appendicular
T3820	15277-15285	NN	denotes	skeleton
T3822	15286-15288	IN	denotes	of
T3823	15289-15294	DT	denotes	these
T3824	15295-15302	NNS	denotes	animals
T3817	15303-15305	VBZ	denotes	is
T3825	15306-15307	DT	denotes	a
T3827	15308-15322	JJ	denotes	characteristic
T3826	15323-15335	NN	denotes	malformation
T3828	15336-15338	IN	denotes	of
T3829	15339-15342	DT	denotes	the
T3830	15343-15350	NN	denotes	scapula
T3831	15351-15352	-LRB-	denotes	(
T3833	15352-15358	NN	denotes	Figure
T3834	15359-15360	CD	denotes	1
T3835	15360-15362	,	denotes	,
T3836	15362-15365	JJ	denotes	red
T3832	15366-15371	NN	denotes	arrow
T3837	15371-15372	-RRB-	denotes	)
T3838	15372-15373	.	denotes	.
T3839	15373-15471	sentence	denotes	BMP2-deficient limbs also exhibit 3/4 soft-tissue syndactyly with variable penetrance (Figure 2).
T3840	15374-15378	NN	denotes	BMP2
T3842	15378-15379	HYPH	denotes	-
T3841	15379-15388	JJ	denotes	deficient
T3843	15389-15394	NNS	denotes	limbs
T3845	15395-15399	RB	denotes	also
T3844	15400-15407	VBP	denotes	exhibit
T3846	15408-15409	CD	denotes	3
T3848	15409-15410	HYPH	denotes	/
T3847	15410-15411	CD	denotes	4
T3850	15412-15416	JJ	denotes	soft
T3852	15416-15417	HYPH	denotes	-
T3851	15417-15423	NN	denotes	tissue
T3849	15424-15434	NN	denotes	syndactyly
T3853	15435-15439	IN	denotes	with
T3854	15440-15448	JJ	denotes	variable
T3855	15449-15459	NN	denotes	penetrance
T3856	15460-15461	-LRB-	denotes	(
T3857	15461-15467	NN	denotes	Figure
T3858	15468-15469	CD	denotes	2
T3859	15469-15470	-RRB-	denotes	)
T3860	15470-15471	.	denotes	.
T3861	15471-16474	sentence	denotes	Figure 2 Depletion of BMP Signaling Causes Interdigital Syndactyly (A–D) Forelimb of adult wild-type (A) and Bmp2C/C; Prx1::cre mouse (B) and hindlimbs of newborn wild-type (C) mouse and newborn Bmp2C/C; Bmp4C/C; Prx1::cre (D) mouse. The black arrow in (B) shows soft tissue syndactyly in Bmp2C/C; Prx1::cre mouse. (E and F) Wild-type and Bmp2C/C; Bmp4C/C; Prx1::cre, respectively, showing acridine orange–stained hindlimbs of E15.5 mouse embryos. Acridine orange stain is in yellow. (G and H) Enlarged views of selected regions from (E) and (F), respectively. Black arrow in (G) and (H) show acridine orange–stained apoptotic cells in the interdigital mesenchyme, and asterisk in (H) shows the remnant of the AER. (I and J) Fgf8 mRNA expression in the hindlimbs of E13.5 wild-type (I) and Bmp2C/C; Bmp4C/C; Prx1::cre (J) embryos. The thick black arrows in (J) show Fgf8 mRNA expression. Mice with limbs deficient in BMP4 activity and the limbs of Bmp7 mutant mice have both been previously described.
T3862	16361-16365	NNS	denotes	Mice
T3864	16366-16370	IN	denotes	with
T3865	16371-16376	NNS	denotes	limbs
T3866	16377-16386	JJ	denotes	deficient
T3867	16387-16389	IN	denotes	in
T3868	16390-16394	NN	denotes	BMP4
T3869	16395-16403	NN	denotes	activity
T3870	16404-16407	CC	denotes	and
T3871	16408-16411	DT	denotes	the
T3872	16412-16417	NNS	denotes	limbs
T3873	16418-16420	IN	denotes	of
T3874	16421-16425	NN	denotes	Bmp7
T3875	16426-16432	NN	denotes	mutant
T3876	16433-16437	NNS	denotes	mice
T3877	16438-16442	VBP	denotes	have
T3878	16443-16447	RB	denotes	both
T3879	16448-16452	VBN	denotes	been
T3880	16453-16463	RB	denotes	previously
T3863	16464-16473	VBN	denotes	described
T3881	16473-16474	.	denotes	.
T3882	16474-16715	sentence	denotes	As reported [16], in the absence of BMP4 activity, limbs display a variable penetrance of preaxial and postaxial polydactyly, but otherwise, normal digit patterns and apparently normal skeletal differentiation (Figure 1G and 1O) take place.
T3883	16475-16477	IN	denotes	As
T3884	16478-16486	VBN	denotes	reported
T3886	16487-16488	-LRB-	denotes	[
T3887	16488-16490	CD	denotes	16
T3888	16490-16491	-RRB-	denotes	]
T3889	16491-16493	,	denotes	,
T3890	16493-16495	IN	denotes	in
T3891	16496-16499	DT	denotes	the
T3892	16500-16507	NN	denotes	absence
T3893	16508-16510	IN	denotes	of
T3894	16511-16515	NN	denotes	BMP4
T3895	16516-16524	NN	denotes	activity
T3896	16524-16526	,	denotes	,
T3897	16526-16531	NNS	denotes	limbs
T3885	16532-16539	VBP	denotes	display
T3898	16540-16541	DT	denotes	a
T3900	16542-16550	JJ	denotes	variable
T3899	16551-16561	NN	denotes	penetrance
T3901	16562-16564	IN	denotes	of
T3902	16565-16573	JJ	denotes	preaxial
T3904	16574-16577	CC	denotes	and
T3905	16578-16587	JJ	denotes	postaxial
T3903	16588-16599	NN	denotes	polydactyly
T3906	16599-16601	,	denotes	,
T3907	16601-16604	CC	denotes	but
T3908	16605-16614	RB	denotes	otherwise
T3910	16614-16616	,	denotes	,
T3911	16616-16622	JJ	denotes	normal
T3913	16623-16628	NN	denotes	digit
T3912	16629-16637	NNS	denotes	patterns
T3914	16638-16641	CC	denotes	and
T3915	16642-16652	RB	denotes	apparently
T3916	16653-16659	JJ	denotes	normal
T3918	16660-16668	JJ	denotes	skeletal
T3917	16669-16684	NN	denotes	differentiation
T3919	16685-16686	-LRB-	denotes	(
T3921	16686-16692	NN	denotes	Figure
T3920	16693-16695	NN	denotes	1G
T3922	16696-16699	CC	denotes	and
T3923	16700-16702	NN	denotes	1O
T3924	16702-16703	-RRB-	denotes	)
T3909	16704-16708	VBP	denotes	take
T3925	16709-16714	NN	denotes	place
T3926	16714-16715	.	denotes	.
T3927	16715-16887	sentence	denotes	As previously described, mice homozygous for a null mutation in Bmp7 [12] have no defects in the formation of the normal appendicular skeletal elements (Figure 1H and 1P).
T3928	16716-16718	IN	denotes	As
T3930	16719-16729	RB	denotes	previously
T3929	16730-16739	VBN	denotes	described
T3932	16739-16741	,	denotes	,
T3933	16741-16745	NNS	denotes	mice
T3934	16746-16756	JJ	denotes	homozygous
T3935	16757-16760	IN	denotes	for
T3936	16761-16762	DT	denotes	a
T3938	16763-16767	JJ	denotes	null
T3937	16768-16776	NN	denotes	mutation
T3939	16777-16779	IN	denotes	in
T3940	16780-16784	NN	denotes	Bmp7
T3941	16785-16786	-LRB-	denotes	[
T3942	16786-16788	CD	denotes	12
T3943	16788-16789	-RRB-	denotes	]
T3931	16790-16794	VBP	denotes	have
T3944	16795-16797	DT	denotes	no
T3945	16798-16805	NNS	denotes	defects
T3946	16806-16808	IN	denotes	in
T3947	16809-16812	DT	denotes	the
T3948	16813-16822	NN	denotes	formation
T3949	16823-16825	IN	denotes	of
T3950	16826-16829	DT	denotes	the
T3952	16830-16836	JJ	denotes	normal
T3953	16837-16849	JJ	denotes	appendicular
T3954	16850-16858	JJ	denotes	skeletal
T3951	16859-16867	NNS	denotes	elements
T3955	16868-16869	-LRB-	denotes	(
T3957	16869-16875	NN	denotes	Figure
T3956	16876-16878	NN	denotes	1H
T3958	16879-16882	CC	denotes	and
T3959	16883-16885	NN	denotes	1P
T3960	16885-16886	-RRB-	denotes	)
T3961	16886-16887	.	denotes	.
T3962	16887-16953	sentence	denotes	We do occasionally observe preaxial polydactyly in these mutants.
T3963	16888-16890	PRP	denotes	We
T3965	16891-16893	VBP	denotes	do
T3966	16894-16906	RB	denotes	occasionally
T3964	16907-16914	VB	denotes	observe
T3967	16915-16923	JJ	denotes	preaxial
T3968	16924-16935	NN	denotes	polydactyly
T3969	16936-16938	IN	denotes	in
T3970	16939-16944	DT	denotes	these
T3971	16945-16952	NNS	denotes	mutants
T3972	16952-16953	.	denotes	.
T3973	16953-17150	sentence	denotes	Compound heterozygous mice, with one functional copy each of Bmp2 and Bmp4 in the limb (Bmp2+/C; Bmp4+/C; Prx1::cre), show no effect on either limb patterning or skeletogenesis (unpublished data).
T3974	16954-16962	NN	denotes	Compound
T3976	16963-16975	JJ	denotes	heterozygous
T3975	16976-16980	NNS	denotes	mice
T3978	16980-16982	,	denotes	,
T3979	16982-16986	IN	denotes	with
T3980	16987-16990	CD	denotes	one
T3982	16991-17001	JJ	denotes	functional
T3981	17002-17006	NN	denotes	copy
T3983	17007-17011	DT	denotes	each
T3984	17012-17014	IN	denotes	of
T3985	17015-17019	NN	denotes	Bmp2
T3986	17020-17023	CC	denotes	and
T3987	17024-17028	NN	denotes	Bmp4
T3988	17029-17031	IN	denotes	in
T3989	17032-17035	DT	denotes	the
T3990	17036-17040	NN	denotes	limb
T3991	17041-17042	-LRB-	denotes	(
T3993	17042-17046	NN	denotes	Bmp2
T3994	17046-17047	SYM	denotes	+
T3995	17047-17048	HYPH	denotes	/
T3992	17048-17049	NN	denotes	C
T3996	17049-17050	:	denotes	;
T3997	17051-17055	NN	denotes	Bmp4
T3999	17055-17056	SYM	denotes	+
T4000	17056-17057	HYPH	denotes	/
T3998	17057-17058	NN	denotes	C
T4001	17058-17059	:	denotes	;
T4002	17060-17064	NN	denotes	Prx1
T4004	17064-17065	HYPH	denotes	:
T4005	17065-17066	HYPH	denotes	:
T4003	17066-17069	NN	denotes	cre
T4006	17069-17070	-RRB-	denotes	)
T4007	17070-17072	,	denotes	,
T3977	17072-17076	VBP	denotes	show
T4008	17077-17079	DT	denotes	no
T4009	17080-17086	NN	denotes	effect
T4010	17087-17089	IN	denotes	on
T4011	17090-17096	CC	denotes	either
T4013	17097-17101	NN	denotes	limb
T4012	17102-17112	NN	denotes	patterning
T4014	17113-17115	CC	denotes	or
T4015	17116-17130	NN	denotes	skeletogenesis
T4016	17131-17132	-LRB-	denotes	(
T4018	17132-17143	JJ	denotes	unpublished
T4017	17144-17148	NNS	denotes	data
T4019	17148-17149	-RRB-	denotes	)
T4020	17149-17150	.	denotes	.
T4021	17150-17499	sentence	denotes	Similarly, limb skeletons of mice in which both copies of the Bmp4 gene and one copy of the Bmp2 gene have been conditionally removed in the limb (Bmp2+/C; Bmp4C/C; Prx1::cre) are phenotypically normal other than exhibiting the variable penetrance preaxial and postaxial polydactyly seen in mice deficient in BMP4 activity alone (Figure 1J and 1R).
T4022	17151-17160	RB	denotes	Similarly
T4024	17160-17162	,	denotes	,
T4025	17162-17166	NN	denotes	limb
T4026	17167-17176	NNS	denotes	skeletons
T4027	17177-17179	IN	denotes	of
T4028	17180-17184	NNS	denotes	mice
T4029	17185-17187	IN	denotes	in
T4031	17188-17193	WDT	denotes	which
T4032	17194-17198	DT	denotes	both
T4033	17199-17205	NNS	denotes	copies
T4034	17206-17208	IN	denotes	of
T4035	17209-17212	DT	denotes	the
T4037	17213-17217	NN	denotes	Bmp4
T4036	17218-17222	NN	denotes	gene
T4038	17223-17226	CC	denotes	and
T4039	17227-17230	CD	denotes	one
T4040	17231-17235	NN	denotes	copy
T4041	17236-17238	IN	denotes	of
T4042	17239-17242	DT	denotes	the
T4044	17243-17247	NN	denotes	Bmp2
T4043	17248-17252	NN	denotes	gene
T4045	17253-17257	VBP	denotes	have
T4046	17258-17262	VBN	denotes	been
T4047	17263-17276	RB	denotes	conditionally
T4030	17277-17284	VBN	denotes	removed
T4048	17285-17287	IN	denotes	in
T4049	17288-17291	DT	denotes	the
T4050	17292-17296	NN	denotes	limb
T4051	17297-17298	-LRB-	denotes	(
T4052	17298-17302	NN	denotes	Bmp2
T4054	17302-17303	SYM	denotes	+
T4055	17303-17304	HYPH	denotes	/
T4053	17304-17305	NN	denotes	C
T4056	17305-17306	:	denotes	;
T4057	17307-17312	NN	denotes	Bmp4C
T4059	17312-17313	HYPH	denotes	/
T4058	17313-17314	NN	denotes	C
T4060	17314-17315	:	denotes	;
T4061	17316-17320	NN	denotes	Prx1
T4063	17320-17321	HYPH	denotes	:
T4064	17321-17322	HYPH	denotes	:
T4062	17322-17325	NN	denotes	cre
T4065	17325-17326	-RRB-	denotes	)
T4023	17327-17330	VBP	denotes	are
T4066	17331-17345	RB	denotes	phenotypically
T4067	17346-17352	JJ	denotes	normal
T4068	17353-17358	RB	denotes	other
T4069	17359-17363	IN	denotes	than
T4070	17364-17374	VBG	denotes	exhibiting
T4071	17375-17378	DT	denotes	the
T4073	17379-17387	JJ	denotes	variable
T4074	17388-17398	NN	denotes	penetrance
T4075	17399-17407	JJ	denotes	preaxial
T4076	17408-17411	CC	denotes	and
T4077	17412-17421	JJ	denotes	postaxial
T4072	17422-17433	NN	denotes	polydactyly
T4078	17434-17438	VBN	denotes	seen
T4079	17439-17441	IN	denotes	in
T4080	17442-17446	NNS	denotes	mice
T4081	17447-17456	JJ	denotes	deficient
T4082	17457-17459	IN	denotes	in
T4083	17460-17464	NN	denotes	BMP4
T4084	17465-17473	NN	denotes	activity
T4085	17474-17479	RB	denotes	alone
T4086	17480-17481	-LRB-	denotes	(
T4088	17481-17487	NN	denotes	Figure
T4087	17488-17490	NN	denotes	1J
T4089	17491-17494	CC	denotes	and
T4090	17495-17497	NN	denotes	1R
T4091	17497-17498	-RRB-	denotes	)
T4092	17498-17499	.	denotes	.
T4093	17499-17702	sentence	denotes	In contrast, mice in which both copies of Bmp2 and one copy of Bmp4 had been removed (Bmp2C/C; Bmp4+/C; Prx1::cre) showed more severe skeletal defects, including significantly thinner skeletal elements.
T4094	17500-17502	IN	denotes	In
T4096	17503-17511	NN	denotes	contrast
T4097	17511-17513	,	denotes	,
T4098	17513-17517	NNS	denotes	mice
T4099	17518-17520	IN	denotes	in
T4101	17521-17526	WDT	denotes	which
T4102	17527-17531	DT	denotes	both
T4103	17532-17538	NNS	denotes	copies
T4104	17539-17541	IN	denotes	of
T4105	17542-17546	NN	denotes	Bmp2
T4106	17547-17550	CC	denotes	and
T4107	17551-17554	CD	denotes	one
T4108	17555-17559	NN	denotes	copy
T4109	17560-17562	IN	denotes	of
T4110	17563-17567	NN	denotes	Bmp4
T4111	17568-17571	VBD	denotes	had
T4112	17572-17576	VBN	denotes	been
T4100	17577-17584	VBN	denotes	removed
T4113	17585-17586	-LRB-	denotes	(
T4114	17586-17591	NN	denotes	Bmp2C
T4116	17591-17592	HYPH	denotes	/
T4115	17592-17593	NN	denotes	C
T4117	17593-17594	:	denotes	;
T4118	17595-17599	NN	denotes	Bmp4
T4120	17599-17600	SYM	denotes	+
T4121	17600-17601	HYPH	denotes	/
T4119	17601-17602	NN	denotes	C
T4122	17602-17603	:	denotes	;
T4123	17604-17608	NN	denotes	Prx1
T4125	17608-17609	:	denotes	:
T4126	17609-17610	:	denotes	:
T4124	17610-17613	NN	denotes	cre
T4127	17613-17614	-RRB-	denotes	)
T4095	17615-17621	VBD	denotes	showed
T4128	17622-17626	RBR	denotes	more
T4129	17627-17633	JJ	denotes	severe
T4131	17634-17642	JJ	denotes	skeletal
T4130	17643-17650	NNS	denotes	defects
T4132	17650-17652	,	denotes	,
T4133	17652-17661	VBG	denotes	including
T4134	17662-17675	RB	denotes	significantly
T4135	17676-17683	JJR	denotes	thinner
T4137	17684-17692	JJ	denotes	skeletal
T4136	17693-17701	NNS	denotes	elements
T4138	17701-17702	.	denotes	.
T4139	17702-17789	sentence	denotes	However, the digit patterns of those animals are completely normal (Figure 1I and 1Q).
T4140	17703-17710	RB	denotes	However
T4142	17710-17712	,	denotes	,
T4143	17712-17715	DT	denotes	the
T4145	17716-17721	NN	denotes	digit
T4144	17722-17730	NNS	denotes	patterns
T4146	17731-17733	IN	denotes	of
T4147	17734-17739	DT	denotes	those
T4148	17740-17747	NNS	denotes	animals
T4141	17748-17751	VBP	denotes	are
T4149	17752-17762	RB	denotes	completely
T4150	17763-17769	JJ	denotes	normal
T4151	17770-17771	-LRB-	denotes	(
T4153	17771-17777	NN	denotes	Figure
T4152	17778-17780	NN	denotes	1I
T4154	17781-17784	CC	denotes	and
T4155	17785-17787	NN	denotes	1Q
T4156	17787-17788	-RRB-	denotes	)
T4157	17788-17789	.	denotes	.
T4158	17789-17928	sentence	denotes	Animals in which both copies of Bmp2 and Bmp4 were removed (Bmp2C/C; Bmp4C/C; Prx1::cre) had extremely malformed limbs (Figure 1K and 1S).
T4159	17790-17797	NNS	denotes	Animals
T4161	17798-17800	IN	denotes	in
T4163	17801-17806	WDT	denotes	which
T4164	17807-17811	DT	denotes	both
T4165	17812-17818	NNS	denotes	copies
T4166	17819-17821	IN	denotes	of
T4167	17822-17826	NN	denotes	Bmp2
T4168	17827-17830	CC	denotes	and
T4169	17831-17835	NN	denotes	Bmp4
T4170	17836-17840	VBD	denotes	were
T4162	17841-17848	VBN	denotes	removed
T4171	17849-17850	-LRB-	denotes	(
T4172	17850-17855	NN	denotes	Bmp2C
T4174	17855-17856	HYPH	denotes	/
T4173	17856-17857	NN	denotes	C
T4175	17857-17858	:	denotes	;
T4176	17859-17864	NN	denotes	Bmp4C
T4178	17864-17865	HYPH	denotes	/
T4177	17865-17866	NN	denotes	C
T4179	17866-17867	:	denotes	;
T4180	17868-17872	NN	denotes	Prx1
T4182	17872-17873	HYPH	denotes	:
T4183	17873-17874	HYPH	denotes	:
T4181	17874-17877	NN	denotes	cre
T4184	17877-17878	-RRB-	denotes	)
T4160	17879-17882	VBD	denotes	had
T4185	17883-17892	RB	denotes	extremely
T4186	17893-17902	VBN	denotes	malformed
T4187	17903-17908	NNS	denotes	limbs
T4188	17909-17910	-LRB-	denotes	(
T4190	17910-17916	NN	denotes	Figure
T4189	17917-17919	NN	denotes	1K
T4191	17920-17923	CC	denotes	and
T4192	17924-17926	NN	denotes	1S
T4193	17926-17927	-RRB-	denotes	)
T4194	17927-17928	.	denotes	.
T4195	17928-18134	sentence	denotes	They displayed severely short and malformed stylopods; one of the zeugopod elements was almost always missing, and the remaining one was so deformed that it was difficult to identify the element correctly.
T4196	17929-17933	PRP	denotes	They
T4197	17934-17943	VBD	denotes	displayed
T4199	17944-17952	RB	denotes	severely
T4200	17953-17958	JJ	denotes	short
T4202	17959-17962	CC	denotes	and
T4203	17963-17972	JJ	denotes	malformed
T4201	17973-17982	NNS	denotes	stylopods
T4204	17982-17983	:	denotes	;
T4205	17984-17987	CD	denotes	one
T4206	17988-17990	IN	denotes	of
T4207	17991-17994	DT	denotes	the
T4209	17995-18003	NN	denotes	zeugopod
T4208	18004-18012	NNS	denotes	elements
T4198	18013-18016	VBD	denotes	was
T4210	18017-18023	RB	denotes	almost
T4211	18024-18030	RB	denotes	always
T4212	18031-18038	VBG	denotes	missing
T4213	18038-18040	,	denotes	,
T4214	18040-18043	CC	denotes	and
T4215	18044-18047	DT	denotes	the
T4217	18048-18057	VBG	denotes	remaining
T4216	18058-18061	CD	denotes	one
T4218	18062-18065	VBD	denotes	was
T4219	18066-18068	RB	denotes	so
T4220	18069-18077	VBN	denotes	deformed
T4221	18078-18082	IN	denotes	that
T4223	18083-18085	PRP	denotes	it
T4222	18086-18089	VBD	denotes	was
T4224	18090-18099	JJ	denotes	difficult
T4225	18100-18102	TO	denotes	to
T4226	18103-18111	VB	denotes	identify
T4227	18112-18115	DT	denotes	the
T4228	18116-18123	NN	denotes	element
T4229	18124-18133	RB	denotes	correctly
T4230	18133-18134	.	denotes	.
T4231	18134-18265	sentence	denotes	Moreover, the joint articulations are defective such that zeugopod and stylopod elements are fused (see below and Figure S1A–S1C).
T4232	18135-18143	RB	denotes	Moreover
T4234	18143-18145	,	denotes	,
T4235	18145-18148	DT	denotes	the
T4237	18149-18154	NN	denotes	joint
T4236	18155-18168	NNS	denotes	articulations
T4233	18169-18172	VBP	denotes	are
T4238	18173-18182	JJ	denotes	defective
T4239	18183-18187	JJ	denotes	such
T4241	18188-18192	IN	denotes	that
T4242	18193-18201	NN	denotes	zeugopod
T4244	18202-18205	CC	denotes	and
T4245	18206-18214	NN	denotes	stylopod
T4243	18215-18223	NNS	denotes	elements
T4246	18224-18227	VBP	denotes	are
T4240	18228-18233	VBN	denotes	fused
T4247	18234-18235	-LRB-	denotes	(
T4248	18235-18238	VB	denotes	see
T4249	18239-18244	RB	denotes	below
T4251	18245-18248	CC	denotes	and
T4252	18249-18255	NN	denotes	Figure
T4250	18256-18259	NN	denotes	S1A
T4253	18259-18260	SYM	denotes	–
T4254	18260-18263	NN	denotes	S1C
T4255	18263-18264	-RRB-	denotes	)
T4256	18264-18265	.	denotes	.
T4257	18265-18339	sentence	denotes	Interestingly, the autopods are less affected than the proximal elements.
T4258	18266-18279	RB	denotes	Interestingly
T4260	18279-18281	,	denotes	,
T4261	18281-18284	DT	denotes	the
T4262	18285-18293	NNS	denotes	autopods
T4259	18294-18297	VBP	denotes	are
T4263	18298-18302	RBR	denotes	less
T4264	18303-18311	VBN	denotes	affected
T4265	18312-18316	IN	denotes	than
T4266	18317-18320	DT	denotes	the
T4268	18321-18329	JJ	denotes	proximal
T4267	18330-18338	NNS	denotes	elements
T4269	18338-18339	.	denotes	.
T4270	18339-18500	sentence	denotes	Nonetheless, the autopod elements are significantly reduced in size, and strikingly the two posterior-most digits are missing in the forelimbs of these animals.
T4271	18340-18351	RB	denotes	Nonetheless
T4273	18351-18353	,	denotes	,
T4274	18353-18356	DT	denotes	the
T4276	18357-18364	NN	denotes	autopod
T4275	18365-18373	NNS	denotes	elements
T4272	18374-18377	VBP	denotes	are
T4277	18378-18391	RB	denotes	significantly
T4278	18392-18399	JJ	denotes	reduced
T4279	18400-18402	IN	denotes	in
T4280	18403-18407	NN	denotes	size
T4281	18407-18409	,	denotes	,
T4282	18409-18412	CC	denotes	and
T4283	18413-18423	RB	denotes	strikingly
T4285	18424-18427	DT	denotes	the
T4287	18428-18431	CD	denotes	two
T4288	18432-18446	JJS	denotes	posterior-most
T4286	18447-18453	NNS	denotes	digits
T4284	18454-18457	VBP	denotes	are
T4289	18458-18465	JJ	denotes	missing
T4290	18466-18468	IN	denotes	in
T4291	18469-18472	DT	denotes	the
T4292	18473-18482	NNS	denotes	forelimbs
T4293	18483-18485	IN	denotes	of
T4294	18486-18491	DT	denotes	these
T4295	18492-18499	NNS	denotes	animals
T4296	18499-18500	.	denotes	.
T4297	18500-18608	sentence	denotes	Simultaneous removal of BMP2 and BMP7 had far less of an effect than did removal of BMP2 and BMP4 activity.
T4298	18501-18513	JJ	denotes	Simultaneous
T4299	18514-18521	NN	denotes	removal
T4301	18522-18524	IN	denotes	of
T4302	18525-18529	NN	denotes	BMP2
T4303	18530-18533	CC	denotes	and
T4304	18534-18538	NN	denotes	BMP7
T4300	18539-18542	VBD	denotes	had
T4305	18543-18546	RB	denotes	far
T4306	18547-18551	JJR	denotes	less
T4307	18552-18554	IN	denotes	of
T4308	18555-18557	DT	denotes	an
T4309	18558-18564	NN	denotes	effect
T4310	18565-18569	IN	denotes	than
T4311	18570-18573	VBD	denotes	did
T4312	18574-18581	NN	denotes	removal
T4313	18582-18584	IN	denotes	of
T4314	18585-18589	NN	denotes	BMP2
T4316	18590-18593	CC	denotes	and
T4317	18594-18598	NN	denotes	BMP4
T4315	18599-18607	NN	denotes	activity
T4318	18607-18608	.	denotes	.
T4319	18608-18871	sentence	denotes	Compound heterozygous removal of BMP2 and BMP7 (Bmp2+/C, Bmp7+/ −; Prx1::cre) and heterozygous removal of BMP2 with complete removal of BMP7 (Bmp2+/C, Bmp7 −/−; Prx1::cre) were both completely wild-type in skeletal pattern and differentiation (unpublished data).
T4320	18609-18617	NN	denotes	Compound
T4322	18618-18630	JJ	denotes	heterozygous
T4321	18631-18638	NN	denotes	removal
T4324	18639-18641	IN	denotes	of
T4325	18642-18646	NN	denotes	BMP2
T4326	18647-18650	CC	denotes	and
T4327	18651-18655	NN	denotes	BMP7
T4328	18656-18657	-LRB-	denotes	(
T4330	18657-18661	NN	denotes	Bmp2
T4331	18661-18662	SYM	denotes	+
T4332	18662-18663	HYPH	denotes	/
T4329	18663-18664	NN	denotes	C
T4333	18664-18666	,	denotes	,
T4334	18666-18670	NN	denotes	Bmp7
T4335	18670-18671	SYM	denotes	+
T4336	18671-18672	HYPH	denotes	/
T4337	18673-18674	SYM	denotes	−
T4338	18674-18675	:	denotes	;
T4339	18676-18680	NN	denotes	Prx1
T4341	18680-18681	HYPH	denotes	:
T4342	18681-18682	HYPH	denotes	:
T4340	18682-18685	NN	denotes	cre
T4343	18685-18686	-RRB-	denotes	)
T4344	18687-18690	CC	denotes	and
T4345	18691-18703	JJ	denotes	heterozygous
T4346	18704-18711	NN	denotes	removal
T4347	18712-18714	IN	denotes	of
T4348	18715-18719	NN	denotes	BMP2
T4349	18720-18724	IN	denotes	with
T4350	18725-18733	JJ	denotes	complete
T4351	18734-18741	NN	denotes	removal
T4352	18742-18744	IN	denotes	of
T4353	18745-18749	NN	denotes	BMP7
T4354	18750-18751	-LRB-	denotes	(
T4355	18751-18755	NN	denotes	Bmp2
T4357	18755-18756	SYM	denotes	+
T4358	18756-18757	HYPH	denotes	/
T4356	18757-18758	NN	denotes	C
T4359	18758-18760	,	denotes	,
T4360	18760-18764	NN	denotes	Bmp7
T4361	18765-18766	SYM	denotes	−
T4362	18766-18767	HYPH	denotes	/
T4363	18767-18768	SYM	denotes	−
T4364	18768-18769	:	denotes	;
T4365	18770-18774	NN	denotes	Prx1
T4367	18774-18775	HYPH	denotes	:
T4368	18775-18776	HYPH	denotes	:
T4366	18776-18779	NN	denotes	cre
T4369	18779-18780	-RRB-	denotes	)
T4323	18781-18785	VBD	denotes	were
T4370	18786-18790	RB	denotes	both
T4371	18791-18801	RB	denotes	completely
T4373	18802-18806	JJ	denotes	wild
T4374	18806-18807	HYPH	denotes	-
T4372	18807-18811	NN	denotes	type
T4375	18812-18814	IN	denotes	in
T4376	18815-18823	JJ	denotes	skeletal
T4377	18824-18831	NN	denotes	pattern
T4378	18832-18835	CC	denotes	and
T4379	18836-18851	NN	denotes	differentiation
T4380	18852-18853	-LRB-	denotes	(
T4382	18853-18864	JJ	denotes	unpublished
T4381	18865-18869	NNS	denotes	data
T4383	18869-18870	-RRB-	denotes	)
T4384	18870-18871	.	denotes	.
T4385	18871-19067	sentence	denotes	Mice homozygous for removal of Bmp2 and heterozygous for Bmp7 (Bmp2C/C, Bmp7+/ −; Prx1::cre) displayed the same subtle scapular defect seen in Bmp2C/C; Prx1::cre mutants alone (unpublished data).
T4386	18872-18876	NNS	denotes	Mice
T4388	18877-18887	JJ	denotes	homozygous
T4389	18888-18891	IN	denotes	for
T4390	18892-18899	NN	denotes	removal
T4391	18900-18902	IN	denotes	of
T4392	18903-18907	NN	denotes	Bmp2
T4393	18908-18911	CC	denotes	and
T4394	18912-18924	JJ	denotes	heterozygous
T4395	18925-18928	IN	denotes	for
T4396	18929-18933	NN	denotes	Bmp7
T4397	18934-18935	-LRB-	denotes	(
T4399	18935-18940	NN	denotes	Bmp2C
T4400	18940-18941	HYPH	denotes	/
T4398	18941-18942	NN	denotes	C
T4401	18942-18944	,	denotes	,
T4402	18944-18948	NN	denotes	Bmp7
T4403	18948-18949	SYM	denotes	+
T4404	18949-18950	HYPH	denotes	/
T4405	18951-18952	SYM	denotes	−
T4406	18952-18953	:	denotes	;
T4407	18954-18958	NN	denotes	Prx1
T4409	18958-18959	HYPH	denotes	:
T4410	18959-18960	HYPH	denotes	:
T4408	18960-18963	NN	denotes	cre
T4411	18963-18964	-RRB-	denotes	)
T4387	18965-18974	VBD	denotes	displayed
T4412	18975-18978	DT	denotes	the
T4414	18979-18983	JJ	denotes	same
T4415	18984-18990	JJ	denotes	subtle
T4416	18991-18999	JJ	denotes	scapular
T4413	19000-19006	NN	denotes	defect
T4417	19007-19011	VBN	denotes	seen
T4418	19012-19014	IN	denotes	in
T4419	19015-19020	NN	denotes	Bmp2C
T4421	19020-19021	HYPH	denotes	/
T4420	19021-19022	NN	denotes	C
T4423	19022-19023	:	denotes	;
T4424	19024-19028	NN	denotes	Prx1
T4426	19028-19029	HYPH	denotes	:
T4427	19029-19030	HYPH	denotes	:
T4425	19030-19033	NN	denotes	cre
T4422	19034-19041	NNS	denotes	mutants
T4428	19042-19047	RB	denotes	alone
T4429	19048-19049	-LRB-	denotes	(
T4431	19049-19060	JJ	denotes	unpublished
T4430	19061-19065	NNS	denotes	data
T4432	19065-19066	-RRB-	denotes	)
T4433	19066-19067	.	denotes	.
T4434	19067-19354	sentence	denotes	In limbs developing in the complete absence of both BMP7 and BMP2 activity (Bmp2C/C, Bmp7 −/−; Prx1::cre), the last phalanx was missing from digit III in the forelimb, and these limbs displayed the same phenotype in the hindlimb with variable penetrance (Figure 1L and 1T, black arrow).
T4435	19068-19070	IN	denotes	In
T4437	19071-19076	NNS	denotes	limbs
T4438	19077-19087	VBG	denotes	developing
T4439	19088-19090	IN	denotes	in
T4440	19091-19094	DT	denotes	the
T4442	19095-19103	JJ	denotes	complete
T4441	19104-19111	NN	denotes	absence
T4443	19112-19114	IN	denotes	of
T4444	19115-19119	CC	denotes	both
T4445	19120-19124	NN	denotes	BMP7
T4447	19125-19128	CC	denotes	and
T4448	19129-19133	NN	denotes	BMP2
T4446	19134-19142	NN	denotes	activity
T4449	19143-19144	-LRB-	denotes	(
T4451	19144-19149	NN	denotes	Bmp2C
T4452	19149-19150	HYPH	denotes	/
T4450	19150-19151	NN	denotes	C
T4453	19151-19153	,	denotes	,
T4454	19153-19157	NN	denotes	Bmp7
T4455	19158-19159	SYM	denotes	−
T4456	19159-19160	HYPH	denotes	/
T4457	19160-19161	SYM	denotes	−
T4458	19161-19162	:	denotes	;
T4459	19163-19167	NN	denotes	Prx1
T4461	19167-19168	HYPH	denotes	:
T4462	19168-19169	HYPH	denotes	:
T4460	19169-19172	NN	denotes	cre
T4463	19172-19173	-RRB-	denotes	)
T4464	19173-19175	,	denotes	,
T4465	19175-19178	DT	denotes	the
T4467	19179-19183	JJ	denotes	last
T4466	19184-19191	NN	denotes	phalanx
T4436	19192-19195	VBD	denotes	was
T4468	19196-19203	VBG	denotes	missing
T4469	19204-19208	IN	denotes	from
T4470	19209-19214	NN	denotes	digit
T4471	19215-19218	CD	denotes	III
T4472	19219-19221	IN	denotes	in
T4473	19222-19225	DT	denotes	the
T4474	19226-19234	NN	denotes	forelimb
T4475	19234-19236	,	denotes	,
T4476	19236-19239	CC	denotes	and
T4477	19240-19245	DT	denotes	these
T4478	19246-19251	NNS	denotes	limbs
T4479	19252-19261	VBD	denotes	displayed
T4480	19262-19265	DT	denotes	the
T4482	19266-19270	JJ	denotes	same
T4481	19271-19280	NN	denotes	phenotype
T4483	19281-19283	IN	denotes	in
T4484	19284-19287	DT	denotes	the
T4485	19288-19296	NN	denotes	hindlimb
T4486	19297-19301	IN	denotes	with
T4487	19302-19310	JJ	denotes	variable
T4488	19311-19321	NN	denotes	penetrance
T4489	19322-19323	-LRB-	denotes	(
T4491	19323-19329	NN	denotes	Figure
T4492	19330-19332	NN	denotes	1L
T4493	19333-19336	CC	denotes	and
T4494	19337-19339	NN	denotes	1T
T4495	19339-19341	,	denotes	,
T4496	19341-19346	JJ	denotes	black
T4490	19347-19352	NN	denotes	arrow
T4497	19352-19353	-RRB-	denotes	)
T4498	19353-19354	.	denotes	.
T4499	19354-19492	sentence	denotes	Additionally, the fibulae of these hindlimbs are malformed and do not articulate with the femur at the knee (Figure 1T, thick red arrow).
T4500	19355-19367	RB	denotes	Additionally
T4502	19367-19369	,	denotes	,
T4503	19369-19372	DT	denotes	the
T4504	19373-19380	NNS	denotes	fibulae
T4505	19381-19383	IN	denotes	of
T4506	19384-19389	DT	denotes	these
T4507	19390-19399	NNS	denotes	hindlimbs
T4501	19400-19403	VBP	denotes	are
T4508	19404-19413	JJ	denotes	malformed
T4509	19414-19417	CC	denotes	and
T4510	19418-19420	VBP	denotes	do
T4512	19421-19424	RB	denotes	not
T4511	19425-19435	VB	denotes	articulate
T4513	19436-19440	IN	denotes	with
T4514	19441-19444	DT	denotes	the
T4515	19445-19450	NN	denotes	femur
T4516	19451-19453	IN	denotes	at
T4517	19454-19457	DT	denotes	the
T4518	19458-19462	NN	denotes	knee
T4519	19463-19464	-LRB-	denotes	(
T4521	19464-19470	NN	denotes	Figure
T4522	19471-19473	NN	denotes	1T
T4523	19473-19475	,	denotes	,
T4524	19475-19480	JJ	denotes	thick
T4525	19481-19484	JJ	denotes	red
T4520	19485-19490	NN	denotes	arrow
T4526	19490-19491	-RRB-	denotes	)
T4527	19491-19492	.	denotes	.
T4528	19492-19643	sentence	denotes	They also display the same scapular defects seen in Bmp2C/C; Prx1::cre mice, and the overall size of the appendicular skeleton is slightly diminished.
T4529	19493-19497	PRP	denotes	They
T4531	19498-19502	RB	denotes	also
T4530	19503-19510	VBP	denotes	display
T4532	19511-19514	DT	denotes	the
T4534	19515-19519	JJ	denotes	same
T4535	19520-19528	JJ	denotes	scapular
T4533	19529-19536	NNS	denotes	defects
T4536	19537-19541	VBN	denotes	seen
T4537	19542-19544	IN	denotes	in
T4538	19545-19550	NN	denotes	Bmp2C
T4540	19550-19551	HYPH	denotes	/
T4539	19551-19552	NN	denotes	C
T4542	19552-19553	:	denotes	;
T4543	19554-19558	NN	denotes	Prx1
T4545	19558-19559	HYPH	denotes	:
T4546	19559-19560	HYPH	denotes	:
T4544	19560-19563	NN	denotes	cre
T4541	19564-19568	NNS	denotes	mice
T4547	19568-19570	,	denotes	,
T4548	19570-19573	CC	denotes	and
T4549	19574-19577	DT	denotes	the
T4551	19578-19585	JJ	denotes	overall
T4550	19586-19590	NN	denotes	size
T4553	19591-19593	IN	denotes	of
T4554	19594-19597	DT	denotes	the
T4556	19598-19610	JJ	denotes	appendicular
T4555	19611-19619	NN	denotes	skeleton
T4552	19620-19622	VBZ	denotes	is
T4557	19623-19631	RB	denotes	slightly
T4558	19632-19642	JJ	denotes	diminished
T4559	19642-19643	.	denotes	.
T4560	19643-19723	sentence	denotes	However, skeletal differentiation appears normal in the limbs of these animals.
T4561	19644-19651	RB	denotes	However
T4563	19651-19653	,	denotes	,
T4564	19653-19661	JJ	denotes	skeletal
T4565	19662-19677	NN	denotes	differentiation
T4562	19678-19685	VBZ	denotes	appears
T4566	19686-19692	JJ	denotes	normal
T4567	19693-19695	IN	denotes	in
T4568	19696-19699	DT	denotes	the
T4569	19700-19705	NNS	denotes	limbs
T4570	19706-19708	IN	denotes	of
T4571	19709-19714	DT	denotes	these
T4572	19715-19722	NNS	denotes	animals
T4573	19722-19723	.	denotes	.
T4773	19725-19729	JJ	denotes	Soft
T4774	19730-19736	NN	denotes	Tissue
T4775	19737-19747	NN	denotes	Syndactyly
T4776	19748-19756	VBN	denotes	Observed
T4777	19757-19759	IN	denotes	in
T4778	19760-19765	NNS	denotes	Limbs
T4779	19766-19768	IN	denotes	of
T4780	19769-19773	NNS	denotes	Mice
T4781	19774-19780	JJ	denotes	Devoid
T4782	19781-19783	IN	denotes	of
T4783	19784-19788	NN	denotes	BMP2
T4785	19789-19792	CC	denotes	and
T4786	19793-19797	NN	denotes	BMP4
T4784	19798-19806	NN	denotes	Activity
T4787	19806-19924	sentence	denotes	As noted above, Bmp2-deficient animals display a variably penetrant 3/4 soft tissue syndactyly phenotype (Figure 2B).
T4788	19807-19809	IN	denotes	As
T4789	19810-19815	VBN	denotes	noted
T4791	19816-19821	RB	denotes	above
T4792	19821-19823	,	denotes	,
T4793	19823-19827	NN	denotes	Bmp2
T4795	19827-19828	HYPH	denotes	-
T4794	19828-19837	JJ	denotes	deficient
T4796	19838-19845	NNS	denotes	animals
T4790	19846-19853	VBP	denotes	display
T4797	19854-19855	DT	denotes	a
T4799	19856-19864	RB	denotes	variably
T4800	19865-19874	JJ	denotes	penetrant
T4801	19875-19876	CD	denotes	3
T4803	19876-19877	SYM	denotes	/
T4802	19877-19878	CD	denotes	4
T4805	19879-19883	JJ	denotes	soft
T4804	19884-19890	NN	denotes	tissue
T4806	19891-19901	NN	denotes	syndactyly
T4798	19902-19911	NN	denotes	phenotype
T4807	19912-19913	-LRB-	denotes	(
T4809	19913-19919	NN	denotes	Figure
T4808	19920-19922	NN	denotes	2B
T4810	19922-19923	-RRB-	denotes	)
T4811	19923-19924	.	denotes	.
T4812	19924-20008	sentence	denotes	In contrast, Bmp4-deficient mice show no evidence of syndactyly (unpublished data).
T4813	19925-19927	IN	denotes	In
T4815	19928-19936	NN	denotes	contrast
T4816	19936-19938	,	denotes	,
T4817	19938-19942	NN	denotes	Bmp4
T4819	19942-19943	HYPH	denotes	-
T4818	19943-19952	JJ	denotes	deficient
T4820	19953-19957	NNS	denotes	mice
T4814	19958-19962	VBP	denotes	show
T4821	19963-19965	DT	denotes	no
T4822	19966-19974	NN	denotes	evidence
T4823	19975-19977	IN	denotes	of
T4824	19978-19988	NN	denotes	syndactyly
T4825	19989-19990	-LRB-	denotes	(
T4827	19990-20001	JJ	denotes	unpublished
T4826	20002-20006	NNS	denotes	data
T4828	20006-20007	-RRB-	denotes	)
T4829	20007-20008	.	denotes	.
T4830	20008-20257	sentence	denotes	However, one of the striking defects observed in the Bmp2C/C; Bmp4C/C; Prx1::cre mice is that in the newborn animals, the digits of both forelimb (unpublished data) and hindlimb show complete syndactyly (Figure 2D; compare to wild-type, Figure 2C).
T4831	20009-20016	RB	denotes	However
T4833	20016-20018	,	denotes	,
T4834	20018-20021	CD	denotes	one
T4835	20022-20024	IN	denotes	of
T4836	20025-20028	DT	denotes	the
T4838	20029-20037	JJ	denotes	striking
T4837	20038-20045	NNS	denotes	defects
T4839	20046-20054	VBN	denotes	observed
T4840	20055-20057	IN	denotes	in
T4841	20058-20061	DT	denotes	the
T4843	20062-20067	NN	denotes	Bmp2C
T4845	20067-20068	HYPH	denotes	/
T4844	20068-20069	NN	denotes	C
T4846	20069-20070	:	denotes	;
T4847	20071-20076	NN	denotes	Bmp4C
T4849	20076-20077	HYPH	denotes	/
T4848	20077-20078	NN	denotes	C
T4850	20078-20079	:	denotes	;
T4851	20080-20084	NN	denotes	Prx1
T4853	20084-20085	HYPH	denotes	:
T4854	20085-20086	HYPH	denotes	:
T4852	20086-20089	NN	denotes	cre
T4842	20090-20094	NNS	denotes	mice
T4832	20095-20097	VBZ	denotes	is
T4855	20098-20102	IN	denotes	that
T4857	20103-20105	IN	denotes	in
T4858	20106-20109	DT	denotes	the
T4860	20110-20117	JJ	denotes	newborn
T4859	20118-20125	NNS	denotes	animals
T4861	20125-20127	,	denotes	,
T4862	20127-20130	DT	denotes	the
T4863	20131-20137	NNS	denotes	digits
T4864	20138-20140	IN	denotes	of
T4865	20141-20145	CC	denotes	both
T4866	20146-20154	NN	denotes	forelimb
T4867	20155-20156	-LRB-	denotes	(
T4869	20156-20167	JJ	denotes	unpublished
T4868	20168-20172	NNS	denotes	data
T4870	20172-20173	-RRB-	denotes	)
T4871	20174-20177	CC	denotes	and
T4872	20178-20186	NN	denotes	hindlimb
T4856	20187-20191	VBP	denotes	show
T4873	20192-20200	JJ	denotes	complete
T4874	20201-20211	NN	denotes	syndactyly
T4875	20212-20213	-LRB-	denotes	(
T4877	20213-20219	NN	denotes	Figure
T4876	20220-20222	NN	denotes	2D
T4878	20222-20223	:	denotes	;
T4879	20224-20231	VB	denotes	compare
T4880	20232-20234	IN	denotes	to
T4881	20235-20239	JJ	denotes	wild
T4883	20239-20240	HYPH	denotes	-
T4882	20240-20244	NN	denotes	type
T4884	20244-20246	,	denotes	,
T4885	20246-20252	NN	denotes	Figure
T4886	20253-20255	NN	denotes	2C
T4887	20255-20256	-RRB-	denotes	)
T4888	20256-20257	.	denotes	.
T4889	20257-20412	sentence	denotes	To determine the origin of this defect, we examined embryonic limbs at E15.5, the stage when interdigital separation is normally taking place (Figure 2E).
T4890	20258-20260	TO	denotes	To
T4891	20261-20270	VB	denotes	determine
T4893	20271-20274	DT	denotes	the
T4894	20275-20281	NN	denotes	origin
T4895	20282-20284	IN	denotes	of
T4896	20285-20289	DT	denotes	this
T4897	20290-20296	NN	denotes	defect
T4898	20296-20298	,	denotes	,
T4899	20298-20300	PRP	denotes	we
T4892	20301-20309	VBD	denotes	examined
T4900	20310-20319	JJ	denotes	embryonic
T4901	20320-20325	NNS	denotes	limbs
T4902	20326-20328	IN	denotes	at
T4903	20329-20334	NN	denotes	E15.5
T4904	20334-20336	,	denotes	,
T4905	20336-20339	DT	denotes	the
T4906	20340-20345	NN	denotes	stage
T4907	20346-20350	WRB	denotes	when
T4909	20351-20363	JJ	denotes	interdigital
T4910	20364-20374	NN	denotes	separation
T4911	20375-20377	VBZ	denotes	is
T4912	20378-20386	RB	denotes	normally
T4908	20387-20393	VBG	denotes	taking
T4913	20394-20399	NN	denotes	place
T4914	20400-20401	-LRB-	denotes	(
T4916	20401-20407	NN	denotes	Figure
T4915	20408-20410	NN	denotes	2E
T4917	20410-20411	-RRB-	denotes	)
T4918	20411-20412	.	denotes	.
T4919	20412-20575	sentence	denotes	In Bmp2C/C; Bmp4C/C; Prx1::cre limbs, only the very distal tips of each digit were separated, with the autopod adopting the shape of a notched pallet (Figure 2F).
T4920	20413-20415	IN	denotes	In
T4922	20416-20421	NN	denotes	Bmp2C
T4924	20421-20422	HYPH	denotes	/
T4923	20422-20423	NN	denotes	C
T4926	20423-20424	:	denotes	;
T4927	20425-20430	NN	denotes	Bmp4C
T4929	20430-20431	HYPH	denotes	/
T4928	20431-20432	NN	denotes	C
T4930	20432-20433	:	denotes	;
T4931	20434-20438	NN	denotes	Prx1
T4933	20438-20439	HYPH	denotes	:
T4934	20439-20440	HYPH	denotes	:
T4932	20440-20443	NN	denotes	cre
T4925	20444-20449	NNS	denotes	limbs
T4935	20449-20451	,	denotes	,
T4936	20451-20455	RB	denotes	only
T4938	20456-20459	DT	denotes	the
T4939	20460-20464	RB	denotes	very
T4940	20465-20471	JJ	denotes	distal
T4937	20472-20476	NNS	denotes	tips
T4941	20477-20479	IN	denotes	of
T4942	20480-20484	DT	denotes	each
T4943	20485-20490	NN	denotes	digit
T4944	20491-20495	VBD	denotes	were
T4921	20496-20505	VBN	denotes	separated
T4945	20505-20507	,	denotes	,
T4946	20507-20511	IN	denotes	with
T4947	20512-20515	DT	denotes	the
T4948	20516-20523	NN	denotes	autopod
T4949	20524-20532	VBG	denotes	adopting
T4950	20533-20536	DT	denotes	the
T4951	20537-20542	NN	denotes	shape
T4952	20543-20545	IN	denotes	of
T4953	20546-20547	DT	denotes	a
T4955	20548-20555	VBN	denotes	notched
T4954	20556-20562	NN	denotes	pallet
T4956	20563-20564	-LRB-	denotes	(
T4958	20564-20570	NN	denotes	Figure
T4957	20571-20573	NN	denotes	2F
T4959	20573-20574	-RRB-	denotes	)
T4960	20574-20575	.	denotes	.
T4961	20575-20768	sentence	denotes	In wild-type development, the individual digits become free from one another through a process of interdigital apoptosis, observable by staining with acridine orange (Figure 2E and 2G, arrow).
T4962	20576-20578	IN	denotes	In
T4964	20579-20583	JJ	denotes	wild
T4966	20583-20584	HYPH	denotes	-
T4965	20584-20588	NN	denotes	type
T4967	20589-20600	NN	denotes	development
T4968	20600-20602	,	denotes	,
T4969	20602-20605	DT	denotes	the
T4971	20606-20616	JJ	denotes	individual
T4970	20617-20623	NNS	denotes	digits
T4963	20624-20630	VBP	denotes	become
T4972	20631-20635	JJ	denotes	free
T4973	20636-20640	IN	denotes	from
T4974	20641-20644	CD	denotes	one
T4975	20645-20652	DT	denotes	another
T4976	20653-20660	IN	denotes	through
T4977	20661-20662	DT	denotes	a
T4978	20663-20670	NN	denotes	process
T4979	20671-20673	IN	denotes	of
T4980	20674-20686	JJ	denotes	interdigital
T4981	20687-20696	NN	denotes	apoptosis
T4982	20696-20698	,	denotes	,
T4983	20698-20708	JJ	denotes	observable
T4984	20709-20711	IN	denotes	by
T4985	20712-20720	VBG	denotes	staining
T4986	20721-20725	IN	denotes	with
T4987	20726-20734	JJ	denotes	acridine
T4988	20735-20741	NN	denotes	orange
T4989	20742-20743	-LRB-	denotes	(
T4991	20743-20749	NN	denotes	Figure
T4992	20750-20752	NN	denotes	2E
T4993	20753-20756	CC	denotes	and
T4994	20757-20759	NN	denotes	2G
T4995	20759-20761	,	denotes	,
T4990	20761-20766	NN	denotes	arrow
T4996	20766-20767	-RRB-	denotes	)
T4997	20767-20768	.	denotes	.
T4998	20768-20883	sentence	denotes	Interdigital apoptosis is dramatically reduced in the Bmp2C/C; Bmp4C/C; Prx1::cre limbs (Figure 2F and 2H, arrow).
T4999	20769-20781	JJ	denotes	Interdigital
T5000	20782-20791	NN	denotes	apoptosis
T5002	20792-20794	VBZ	denotes	is
T5003	20795-20807	RB	denotes	dramatically
T5001	20808-20815	VBN	denotes	reduced
T5004	20816-20818	IN	denotes	in
T5005	20819-20822	DT	denotes	the
T5007	20823-20828	NN	denotes	Bmp2C
T5009	20828-20829	HYPH	denotes	/
T5008	20829-20830	NN	denotes	C
T5010	20830-20831	:	denotes	;
T5011	20832-20837	NN	denotes	Bmp4C
T5013	20837-20838	HYPH	denotes	/
T5012	20838-20839	NN	denotes	C
T5014	20839-20840	:	denotes	;
T5015	20841-20845	NN	denotes	Prx1
T5017	20845-20846	HYPH	denotes	:
T5018	20846-20847	HYPH	denotes	:
T5016	20847-20850	NN	denotes	cre
T5006	20851-20856	NNS	denotes	limbs
T5019	20857-20858	-LRB-	denotes	(
T5021	20858-20864	NN	denotes	Figure
T5022	20865-20867	NN	denotes	2F
T5023	20868-20871	CC	denotes	and
T5024	20872-20874	NN	denotes	2H
T5025	20874-20876	,	denotes	,
T5020	20876-20881	NN	denotes	arrow
T5026	20881-20882	-RRB-	denotes	)
T5027	20882-20883	.	denotes	.
T5028	20883-21051	sentence	denotes	(Note that the strong acridine orange staining in Figure 2H is not in the interdigital mesenchyme but rather is in the remnant of the cells of the AER [red asterisk].)
T5029	20884-20885	-LRB-	denotes	(
T5030	20885-20889	VB	denotes	Note
T5031	20890-20894	IN	denotes	that
T5033	20895-20898	DT	denotes	the
T5035	20899-20905	JJ	denotes	strong
T5036	20906-20914	NN	denotes	acridine
T5037	20915-20921	NN	denotes	orange
T5034	20922-20930	NN	denotes	staining
T5038	20931-20933	IN	denotes	in
T5039	20934-20940	NN	denotes	Figure
T5040	20941-20943	NN	denotes	2H
T5032	20944-20946	VBZ	denotes	is
T5041	20947-20950	RB	denotes	not
T5042	20951-20953	IN	denotes	in
T5043	20954-20957	DT	denotes	the
T5045	20958-20970	JJ	denotes	interdigital
T5044	20971-20981	NN	denotes	mesenchyme
T5046	20982-20985	CC	denotes	but
T5047	20986-20992	RB	denotes	rather
T5048	20993-20995	VBZ	denotes	is
T5049	20996-20998	IN	denotes	in
T5050	20999-21002	DT	denotes	the
T5051	21003-21010	NN	denotes	remnant
T5052	21011-21013	IN	denotes	of
T5053	21014-21017	DT	denotes	the
T5054	21018-21023	NNS	denotes	cells
T5055	21024-21026	IN	denotes	of
T5056	21027-21030	DT	denotes	the
T5057	21031-21034	NN	denotes	AER
T5058	21035-21036	-LRB-	denotes	[
T5060	21036-21039	JJ	denotes	red
T5059	21040-21048	NN	denotes	asterisk
T5061	21048-21049	-RRB-	denotes	]
T5062	21049-21050	.	denotes	.
T5063	21050-21051	-RRB-	denotes	)
T5064	21051-21139	sentence	denotes	Another region of very prominent apoptosis in the wild-type limb bud is within the AER.
T5065	21052-21059	DT	denotes	Another
T5066	21060-21066	NN	denotes	region
T5068	21067-21069	IN	denotes	of
T5069	21070-21074	RB	denotes	very
T5070	21075-21084	JJ	denotes	prominent
T5071	21085-21094	NN	denotes	apoptosis
T5072	21095-21097	IN	denotes	in
T5073	21098-21101	DT	denotes	the
T5075	21102-21106	JJ	denotes	wild
T5077	21106-21107	HYPH	denotes	-
T5076	21107-21111	NN	denotes	type
T5078	21112-21116	NN	denotes	limb
T5074	21117-21120	NN	denotes	bud
T5067	21121-21123	VBZ	denotes	is
T5079	21124-21130	IN	denotes	within
T5080	21131-21134	DT	denotes	the
T5081	21135-21138	NN	denotes	AER
T5082	21138-21139	.	denotes	.
T5083	21139-21268	sentence	denotes	The AER is a specialized ridge of ectoderm running along the anterior-posterior axis at the distal margin of the early limb bud.
T5084	21140-21143	DT	denotes	The
T5085	21144-21147	NN	denotes	AER
T5086	21148-21150	VBZ	denotes	is
T5087	21151-21152	DT	denotes	a
T5089	21153-21164	VBN	denotes	specialized
T5088	21165-21170	NN	denotes	ridge
T5090	21171-21173	IN	denotes	of
T5091	21174-21182	NN	denotes	ectoderm
T5092	21183-21190	VBG	denotes	running
T5093	21191-21196	IN	denotes	along
T5094	21197-21200	DT	denotes	the
T5096	21201-21209	JJ	denotes	anterior
T5098	21209-21210	HYPH	denotes	-
T5097	21210-21219	JJ	denotes	posterior
T5095	21220-21224	NN	denotes	axis
T5099	21225-21227	IN	denotes	at
T5100	21228-21231	DT	denotes	the
T5102	21232-21238	JJ	denotes	distal
T5101	21239-21245	NN	denotes	margin
T5103	21246-21248	IN	denotes	of
T5104	21249-21252	DT	denotes	the
T5106	21253-21258	JJ	denotes	early
T5107	21259-21263	NN	denotes	limb
T5105	21264-21267	NN	denotes	bud
T5108	21267-21268	.	denotes	.
T5109	21268-21381	sentence	denotes	One of the phenotypes characterizing the Bmp2C/C; Bmp4C/C; Prx1::cre mutant is expansion of the AER (see below).
T5110	21269-21272	CD	denotes	One
T5112	21273-21275	IN	denotes	of
T5113	21276-21279	DT	denotes	the
T5114	21280-21290	NNS	denotes	phenotypes
T5115	21291-21305	VBG	denotes	characterizing
T5116	21306-21309	DT	denotes	the
T5118	21310-21315	NN	denotes	Bmp2C
T5120	21315-21316	HYPH	denotes	/
T5119	21316-21317	NN	denotes	C
T5121	21317-21318	:	denotes	;
T5122	21319-21324	NN	denotes	Bmp4C
T5124	21324-21325	HYPH	denotes	/
T5123	21325-21326	NN	denotes	C
T5125	21326-21327	:	denotes	;
T5126	21328-21332	NN	denotes	Prx1
T5128	21332-21333	HYPH	denotes	:
T5129	21333-21334	HYPH	denotes	:
T5127	21334-21337	NN	denotes	cre
T5117	21338-21344	NN	denotes	mutant
T5111	21345-21347	VBZ	denotes	is
T5130	21348-21357	NN	denotes	expansion
T5131	21358-21360	IN	denotes	of
T5132	21361-21364	DT	denotes	the
T5133	21365-21368	NN	denotes	AER
T5134	21369-21370	-LRB-	denotes	(
T5135	21370-21373	VB	denotes	see
T5136	21374-21379	RB	denotes	below
T5137	21379-21380	-RRB-	denotes	)
T5138	21380-21381	.	denotes	.
T5139	21381-21532	sentence	denotes	Moreover, in the Bmp2C/C; Bmp4C/C; Prx1::cre animals, the AER is maintained as late as E15.5, long after it has started to degrade in wild-type limbs.
T5140	21382-21390	RB	denotes	Moreover
T5142	21390-21392	,	denotes	,
T5143	21392-21394	IN	denotes	in
T5144	21395-21398	DT	denotes	the
T5146	21399-21404	NN	denotes	Bmp2C
T5148	21404-21405	HYPH	denotes	/
T5147	21405-21406	NN	denotes	C
T5149	21406-21407	:	denotes	;
T5150	21408-21413	NN	denotes	Bmp4C
T5152	21413-21414	HYPH	denotes	/
T5151	21414-21415	NN	denotes	C
T5153	21415-21416	:	denotes	;
T5154	21417-21421	NN	denotes	Prx1
T5156	21421-21422	HYPH	denotes	:
T5157	21422-21423	HYPH	denotes	:
T5155	21423-21426	NN	denotes	cre
T5145	21427-21434	NNS	denotes	animals
T5158	21434-21436	,	denotes	,
T5159	21436-21439	DT	denotes	the
T5160	21440-21443	NN	denotes	AER
T5161	21444-21446	VBZ	denotes	is
T5141	21447-21457	VBN	denotes	maintained
T5162	21458-21460	RB	denotes	as
T5163	21461-21465	RB	denotes	late
T5164	21466-21468	IN	denotes	as
T5165	21469-21474	NN	denotes	E15.5
T5166	21474-21476	,	denotes	,
T5167	21476-21480	RB	denotes	long
T5169	21481-21486	IN	denotes	after
T5170	21487-21489	PRP	denotes	it
T5171	21490-21493	VBZ	denotes	has
T5168	21494-21501	VBN	denotes	started
T5172	21502-21504	TO	denotes	to
T5173	21505-21512	VB	denotes	degrade
T5174	21513-21515	IN	denotes	in
T5175	21516-21520	JJ	denotes	wild
T5177	21520-21521	HYPH	denotes	-
T5176	21521-21525	NN	denotes	type
T5178	21526-21531	NNS	denotes	limbs
T5179	21531-21532	.	denotes	.
T5180	21532-21670	sentence	denotes	This can be seen morphologically (unpublished data) or by staining for expression of AER-specific marker such as Fgf8 (Figure 2I and 2J).
T5181	21533-21537	DT	denotes	This
T5183	21538-21541	MD	denotes	can
T5184	21542-21544	VB	denotes	be
T5182	21545-21549	VBN	denotes	seen
T5185	21550-21565	RB	denotes	morphologically
T5187	21566-21567	-LRB-	denotes	(
T5189	21567-21578	JJ	denotes	unpublished
T5188	21579-21583	NNS	denotes	data
T5190	21583-21584	-RRB-	denotes	)
T5191	21585-21587	CC	denotes	or
T5186	21588-21590	IN	denotes	by
T5192	21591-21599	VBG	denotes	staining
T5193	21600-21603	IN	denotes	for
T5194	21604-21614	NN	denotes	expression
T5195	21615-21617	IN	denotes	of
T5196	21618-21621	NN	denotes	AER
T5198	21621-21622	HYPH	denotes	-
T5199	21622-21630	JJ	denotes	specific
T5197	21631-21637	NN	denotes	marker
T5200	21638-21642	JJ	denotes	such
T5201	21643-21645	IN	denotes	as
T5202	21646-21650	NN	denotes	Fgf8
T5203	21651-21652	-LRB-	denotes	(
T5205	21652-21658	NN	denotes	Figure
T5204	21659-21661	NN	denotes	2I
T5206	21662-21665	CC	denotes	and
T5207	21666-21668	NN	denotes	2J
T5208	21668-21669	-RRB-	denotes	)
T5209	21669-21670	.	denotes	.
T5210	21670-21865	sentence	denotes	The AER displays intense staining with acridine orange, TUNEL, or other markers for apoptosis at every stage when it is present, reflecting the rapid turnover of cells in this structure [33–35].
T5211	21671-21674	DT	denotes	The
T5212	21675-21678	NN	denotes	AER
T5213	21679-21687	VBZ	denotes	displays
T5214	21688-21695	JJ	denotes	intense
T5215	21696-21704	NN	denotes	staining
T5216	21705-21709	IN	denotes	with
T5217	21710-21718	NN	denotes	acridine
T5218	21719-21725	NN	denotes	orange
T5219	21725-21727	,	denotes	,
T5220	21727-21732	NN	denotes	TUNEL
T5221	21732-21734	,	denotes	,
T5222	21734-21736	CC	denotes	or
T5223	21737-21742	JJ	denotes	other
T5224	21743-21750	NNS	denotes	markers
T5225	21751-21754	IN	denotes	for
T5226	21755-21764	NN	denotes	apoptosis
T5227	21765-21767	IN	denotes	at
T5228	21768-21773	DT	denotes	every
T5229	21774-21779	NN	denotes	stage
T5230	21780-21784	WRB	denotes	when
T5232	21785-21787	PRP	denotes	it
T5231	21788-21790	VBZ	denotes	is
T5233	21791-21798	JJ	denotes	present
T5234	21798-21800	,	denotes	,
T5235	21800-21810	VBG	denotes	reflecting
T5236	21811-21814	DT	denotes	the
T5238	21815-21820	JJ	denotes	rapid
T5237	21821-21829	NN	denotes	turnover
T5239	21830-21832	IN	denotes	of
T5240	21833-21838	NNS	denotes	cells
T5241	21839-21841	IN	denotes	in
T5242	21842-21846	DT	denotes	this
T5243	21847-21856	NN	denotes	structure
T5244	21857-21858	-LRB-	denotes	[
T5245	21858-21860	CD	denotes	33
T5246	21860-21861	SYM	denotes	–
T5247	21861-21863	CD	denotes	35
T5248	21863-21864	-RRB-	denotes	]
T5249	21864-21865	.	denotes	.
T5250	21865-22105	sentence	denotes	The Bmp2C/C; Bmp4C/C; Prx1::cre mutant limbs at E15.5 show strong acridine orange staining in the remnants of AER (Figure 2H, asterisk), in domains of the distal tip where the AER remains the longest (compare staining in Figure 2F and 2J).
T5251	21866-21869	DT	denotes	The
T5253	21870-21875	NN	denotes	Bmp2C
T5255	21875-21876	HYPH	denotes	/
T5254	21876-21877	NN	denotes	C
T5257	21877-21878	:	denotes	;
T5258	21879-21884	NN	denotes	Bmp4C
T5260	21884-21885	HYPH	denotes	/
T5259	21885-21886	NN	denotes	C
T5261	21886-21887	:	denotes	;
T5262	21888-21892	NN	denotes	Prx1
T5264	21892-21893	HYPH	denotes	:
T5265	21893-21894	HYPH	denotes	:
T5263	21894-21897	NN	denotes	cre
T5256	21898-21904	NN	denotes	mutant
T5252	21905-21910	NNS	denotes	limbs
T5267	21911-21913	IN	denotes	at
T5268	21914-21919	NN	denotes	E15.5
T5266	21920-21924	VBP	denotes	show
T5269	21925-21931	JJ	denotes	strong
T5271	21932-21940	NN	denotes	acridine
T5272	21941-21947	NN	denotes	orange
T5270	21948-21956	NN	denotes	staining
T5273	21957-21959	IN	denotes	in
T5274	21960-21963	DT	denotes	the
T5275	21964-21972	NNS	denotes	remnants
T5276	21973-21975	IN	denotes	of
T5277	21976-21979	NN	denotes	AER
T5278	21980-21981	-LRB-	denotes	(
T5280	21981-21987	NN	denotes	Figure
T5281	21988-21990	NN	denotes	2H
T5282	21990-21992	,	denotes	,
T5279	21992-22000	NN	denotes	asterisk
T5283	22000-22001	-RRB-	denotes	)
T5284	22001-22003	,	denotes	,
T5285	22003-22005	IN	denotes	in
T5286	22006-22013	NNS	denotes	domains
T5287	22014-22016	IN	denotes	of
T5288	22017-22020	DT	denotes	the
T5290	22021-22027	JJ	denotes	distal
T5289	22028-22031	NN	denotes	tip
T5291	22032-22037	WRB	denotes	where
T5293	22038-22041	DT	denotes	the
T5294	22042-22045	NN	denotes	AER
T5292	22046-22053	VBZ	denotes	remains
T5295	22054-22057	DT	denotes	the
T5296	22058-22065	JJS	denotes	longest
T5297	22066-22067	-LRB-	denotes	(
T5298	22067-22074	VBP	denotes	compare
T5299	22075-22083	NN	denotes	staining
T5300	22084-22086	IN	denotes	in
T5301	22087-22093	NN	denotes	Figure
T5302	22094-22096	NN	denotes	2F
T5303	22097-22100	CC	denotes	and
T5304	22101-22103	NN	denotes	2J
T5305	22103-22104	-RRB-	denotes	)
T5306	22104-22105	.	denotes	.
T5307	22105-22281	sentence	denotes	No significant difference was observed in the extent of apoptosis in the cells of the AER in earlier stage Bmp2C/C; Bmp4C/C; Prx1::cre mutant embryonic limbs (Figure S1D–S1G).
T5308	22106-22108	DT	denotes	No
T5310	22109-22120	JJ	denotes	significant
T5309	22121-22131	NN	denotes	difference
T5312	22132-22135	VBD	denotes	was
T5311	22136-22144	VBN	denotes	observed
T5313	22145-22147	IN	denotes	in
T5314	22148-22151	DT	denotes	the
T5315	22152-22158	NN	denotes	extent
T5316	22159-22161	IN	denotes	of
T5317	22162-22171	NN	denotes	apoptosis
T5318	22172-22174	IN	denotes	in
T5319	22175-22178	DT	denotes	the
T5320	22179-22184	NNS	denotes	cells
T5321	22185-22187	IN	denotes	of
T5322	22188-22191	DT	denotes	the
T5323	22192-22195	NN	denotes	AER
T5324	22196-22198	IN	denotes	in
T5325	22199-22206	JJR	denotes	earlier
T5326	22207-22212	NN	denotes	stage
T5328	22213-22218	NN	denotes	Bmp2C
T5330	22218-22219	HYPH	denotes	/
T5329	22219-22220	NN	denotes	C
T5332	22220-22221	:	denotes	;
T5333	22222-22227	NN	denotes	Bmp4C
T5335	22227-22228	HYPH	denotes	/
T5334	22228-22229	NN	denotes	C
T5336	22229-22230	:	denotes	;
T5337	22231-22235	NN	denotes	Prx1
T5339	22235-22236	HYPH	denotes	:
T5340	22236-22237	HYPH	denotes	:
T5338	22237-22240	NN	denotes	cre
T5331	22241-22247	NN	denotes	mutant
T5341	22248-22257	JJ	denotes	embryonic
T5327	22258-22263	NNS	denotes	limbs
T5342	22264-22265	-LRB-	denotes	(
T5344	22265-22271	NN	denotes	Figure
T5343	22272-22275	NN	denotes	S1D
T5345	22275-22276	SYM	denotes	–
T5346	22276-22279	NN	denotes	S1G
T5347	22279-22280	-RRB-	denotes	)
T5348	22280-22281	.	denotes	.
T5836	22283-22293	NN	denotes	Patterning
T5837	22294-22301	NNS	denotes	Defects
T5838	22302-22304	IN	denotes	in
T5839	22305-22309	NN	denotes	Limb
T5840	22310-22320	VBG	denotes	Developing
T5841	22321-22325	IN	denotes	with
T5842	22326-22333	VBN	denotes	Reduced
T5843	22334-22340	NNS	denotes	Levels
T5844	22341-22343	IN	denotes	of
T5845	22344-22347	NN	denotes	BMP
T5846	22348-22357	NN	denotes	Signaling
T5847	22357-22440	sentence	denotes	BMP signaling has been proposed to play several distinct roles in limb patterning.
T5848	22358-22361	NN	denotes	BMP
T5849	22362-22371	NN	denotes	signaling
T5851	22372-22375	VBZ	denotes	has
T5852	22376-22380	VBN	denotes	been
T5850	22381-22389	VBN	denotes	proposed
T5853	22390-22392	TO	denotes	to
T5854	22393-22397	VB	denotes	play
T5855	22398-22405	JJ	denotes	several
T5857	22406-22414	JJ	denotes	distinct
T5856	22415-22420	NNS	denotes	roles
T5858	22421-22423	IN	denotes	in
T5859	22424-22428	NN	denotes	limb
T5860	22429-22439	NN	denotes	patterning
T5861	22439-22440	.	denotes	.
T5862	22440-22572	sentence	denotes	The first of these is in establishing differences along the anterior-posterior axis of the limb downstream of sonic hedgehog (Shh).
T5863	22441-22444	DT	denotes	The
T5864	22445-22450	JJ	denotes	first
T5866	22451-22453	IN	denotes	of
T5867	22454-22459	DT	denotes	these
T5865	22460-22462	VBZ	denotes	is
T5868	22463-22465	IN	denotes	in
T5869	22466-22478	VBG	denotes	establishing
T5870	22479-22490	NNS	denotes	differences
T5871	22491-22496	IN	denotes	along
T5872	22497-22500	DT	denotes	the
T5874	22501-22509	JJ	denotes	anterior
T5876	22509-22510	HYPH	denotes	-
T5875	22510-22519	JJ	denotes	posterior
T5873	22520-22524	NN	denotes	axis
T5877	22525-22527	IN	denotes	of
T5878	22528-22531	DT	denotes	the
T5879	22532-22536	NN	denotes	limb
T5880	22537-22547	RB	denotes	downstream
T5881	22548-22550	IN	denotes	of
T5882	22551-22556	JJ	denotes	sonic
T5883	22557-22565	NN	denotes	hedgehog
T5884	22566-22567	-LRB-	denotes	(
T5885	22567-22570	NN	denotes	Shh
T5886	22570-22571	-RRB-	denotes	)
T5887	22571-22572	.	denotes	.
T5888	22572-22724	sentence	denotes	SHH signaling itself extends over a long range in the developing limb bud, based on both activation of target genes [36] and protein distribution [37].
T5889	22573-22576	NN	denotes	SHH
T5890	22577-22586	NN	denotes	signaling
T5892	22587-22593	PRP	denotes	itself
T5891	22594-22601	VBZ	denotes	extends
T5893	22602-22606	IN	denotes	over
T5894	22607-22608	DT	denotes	a
T5896	22609-22613	JJ	denotes	long
T5895	22614-22619	NN	denotes	range
T5897	22620-22622	IN	denotes	in
T5898	22623-22626	DT	denotes	the
T5900	22627-22637	VBG	denotes	developing
T5901	22638-22642	NN	denotes	limb
T5899	22643-22646	NN	denotes	bud
T5902	22646-22648	,	denotes	,
T5903	22648-22653	VBN	denotes	based
T5904	22654-22656	IN	denotes	on
T5905	22657-22661	CC	denotes	both
T5906	22662-22672	NN	denotes	activation
T5907	22673-22675	IN	denotes	of
T5908	22676-22682	NN	denotes	target
T5909	22683-22688	NNS	denotes	genes
T5910	22689-22690	-LRB-	denotes	[
T5911	22690-22692	CD	denotes	36
T5912	22692-22693	-RRB-	denotes	]
T5913	22694-22697	CC	denotes	and
T5914	22698-22705	NN	denotes	protein
T5915	22706-22718	NN	denotes	distribution
T5916	22719-22720	-LRB-	denotes	[
T5917	22720-22722	CD	denotes	37
T5918	22722-22723	-RRB-	denotes	]
T5919	22723-22724	.	denotes	.
T5920	22724-22927	sentence	denotes	Nonetheless, some models have suggested that the patterning effect of this morphogenic signal is mediated entirely [10] or in part [11] by BMP2 and BMP7 produced as secondary signals in response to SHH.
T5921	22725-22736	RB	denotes	Nonetheless
T5923	22736-22738	,	denotes	,
T5924	22738-22742	DT	denotes	some
T5925	22743-22749	NNS	denotes	models
T5926	22750-22754	VBP	denotes	have
T5922	22755-22764	VBN	denotes	suggested
T5927	22765-22769	IN	denotes	that
T5929	22770-22773	DT	denotes	the
T5931	22774-22784	NN	denotes	patterning
T5930	22785-22791	NN	denotes	effect
T5932	22792-22794	IN	denotes	of
T5933	22795-22799	DT	denotes	this
T5935	22800-22811	JJ	denotes	morphogenic
T5934	22812-22818	NN	denotes	signal
T5936	22819-22821	VBZ	denotes	is
T5928	22822-22830	VBN	denotes	mediated
T5937	22831-22839	RB	denotes	entirely
T5939	22840-22841	-LRB-	denotes	[
T5940	22841-22843	CD	denotes	10
T5941	22843-22844	-RRB-	denotes	]
T5942	22845-22847	CC	denotes	or
T5938	22848-22850	IN	denotes	in
T5943	22851-22855	JJ	denotes	part
T5944	22856-22857	-LRB-	denotes	[
T5945	22857-22859	CD	denotes	11
T5946	22859-22860	-RRB-	denotes	]
T5947	22861-22863	IN	denotes	by
T5948	22864-22868	NN	denotes	BMP2
T5949	22869-22872	CC	denotes	and
T5950	22873-22877	NN	denotes	BMP7
T5951	22878-22886	VBN	denotes	produced
T5952	22887-22889	IN	denotes	as
T5953	22890-22899	JJ	denotes	secondary
T5954	22900-22907	NNS	denotes	signals
T5955	22908-22910	IN	denotes	in
T5956	22911-22919	NN	denotes	response
T5957	22920-22922	IN	denotes	to
T5958	22923-22926	NN	denotes	SHH
T5959	22926-22927	.	denotes	.
T5960	22927-23122	sentence	denotes	We have removed both BMP2 and BMP7 activities in the early limb bud (Figure 1), and moreover, there is no apparent upregulation of BMP4 expression in the posterior of these limbs (Figure 3A–3D).
T5961	22928-22930	PRP	denotes	We
T5963	22931-22935	VBP	denotes	have
T5962	22936-22943	VBN	denotes	removed
T5964	22944-22948	CC	denotes	both
T5965	22949-22953	NN	denotes	BMP2
T5967	22954-22957	CC	denotes	and
T5968	22958-22962	NN	denotes	BMP7
T5966	22963-22973	NNS	denotes	activities
T5969	22974-22976	IN	denotes	in
T5970	22977-22980	DT	denotes	the
T5972	22981-22986	JJ	denotes	early
T5973	22987-22991	NN	denotes	limb
T5971	22992-22995	NN	denotes	bud
T5974	22996-22997	-LRB-	denotes	(
T5975	22997-23003	NN	denotes	Figure
T5976	23004-23005	CD	denotes	1
T5977	23005-23006	-RRB-	denotes	)
T5978	23006-23008	,	denotes	,
T5979	23008-23011	CC	denotes	and
T5980	23012-23020	RB	denotes	moreover
T5982	23020-23022	,	denotes	,
T5983	23022-23027	EX	denotes	there
T5981	23028-23030	VBZ	denotes	is
T5984	23031-23033	DT	denotes	no
T5986	23034-23042	JJ	denotes	apparent
T5985	23043-23055	NN	denotes	upregulation
T5987	23056-23058	IN	denotes	of
T5988	23059-23063	NN	denotes	BMP4
T5989	23064-23074	NN	denotes	expression
T5990	23075-23077	IN	denotes	in
T5991	23078-23081	DT	denotes	the
T5992	23082-23091	NN	denotes	posterior
T5993	23092-23094	IN	denotes	of
T5994	23095-23100	DT	denotes	these
T5995	23101-23106	NNS	denotes	limbs
T5996	23107-23108	-LRB-	denotes	(
T5998	23108-23114	NN	denotes	Figure
T5997	23115-23117	NN	denotes	3A
T5999	23117-23118	SYM	denotes	–
T6000	23118-23120	NN	denotes	3D
T6001	23120-23121	-RRB-	denotes	)
T6002	23121-23122	.	denotes	.
T6003	23122-23310	sentence	denotes	The skeletal phenotypes of mouse limbs formed in the absence of BMP2, BMP7, and both BMP2 and BMP7 are illuminating, as none of these exhibit significant digit pattern defects (Figure 1).
T6004	23123-23126	DT	denotes	The
T6006	23127-23135	JJ	denotes	skeletal
T6005	23136-23146	NNS	denotes	phenotypes
T6008	23147-23149	IN	denotes	of
T6009	23150-23155	NN	denotes	mouse
T6010	23156-23161	NNS	denotes	limbs
T6007	23162-23168	VBN	denotes	formed
T6011	23169-23171	IN	denotes	in
T6012	23172-23175	DT	denotes	the
T6013	23176-23183	NN	denotes	absence
T6014	23184-23186	IN	denotes	of
T6015	23187-23191	NN	denotes	BMP2
T6017	23191-23193	,	denotes	,
T6016	23193-23197	NN	denotes	BMP7
T6018	23197-23199	,	denotes	,
T6019	23199-23202	CC	denotes	and
T6020	23203-23207	CC	denotes	both
T6021	23208-23212	NN	denotes	BMP2
T6023	23213-23216	CC	denotes	and
T6024	23217-23221	NN	denotes	BMP7
T6022	23222-23225	VBP	denotes	are
T6025	23226-23238	VBG	denotes	illuminating
T6026	23238-23240	,	denotes	,
T6027	23240-23242	IN	denotes	as
T6029	23243-23247	NN	denotes	none
T6030	23248-23250	IN	denotes	of
T6031	23251-23256	DT	denotes	these
T6028	23257-23264	VBP	denotes	exhibit
T6032	23265-23276	JJ	denotes	significant
T6034	23277-23282	NN	denotes	digit
T6035	23283-23290	NN	denotes	pattern
T6033	23291-23298	NNS	denotes	defects
T6036	23299-23300	-LRB-	denotes	(
T6037	23300-23306	NN	denotes	Figure
T6038	23307-23308	CD	denotes	1
T6039	23308-23309	-RRB-	denotes	)
T6040	23309-23310	.	denotes	.
T6041	23310-23524	sentence	denotes	While a distal phalanx is missing in the middle digits of the double mutants (Bmp2C/C, Bmp7 −/−; Prx1::cre), this is not a phenotype consistent with a decrease or loss of a posteriorly derived polarizing activity.
T6042	23311-23316	IN	denotes	While
T6044	23317-23318	DT	denotes	a
T6046	23319-23325	JJ	denotes	distal
T6045	23326-23333	NN	denotes	phalanx
T6043	23334-23336	VBZ	denotes	is
T6048	23337-23344	VBG	denotes	missing
T6049	23345-23347	IN	denotes	in
T6050	23348-23351	DT	denotes	the
T6052	23352-23358	JJ	denotes	middle
T6051	23359-23365	NNS	denotes	digits
T6053	23366-23368	IN	denotes	of
T6054	23369-23372	DT	denotes	the
T6056	23373-23379	JJ	denotes	double
T6055	23380-23387	NNS	denotes	mutants
T6057	23388-23389	-LRB-	denotes	(
T6058	23389-23394	NN	denotes	Bmp2C
T6060	23394-23395	HYPH	denotes	/
T6059	23395-23396	NN	denotes	C
T6061	23396-23398	,	denotes	,
T6062	23398-23402	NN	denotes	Bmp7
T6063	23403-23404	SYM	denotes	−
T6064	23404-23405	HYPH	denotes	/
T6065	23405-23406	SYM	denotes	−
T6066	23406-23407	:	denotes	;
T6067	23408-23412	NN	denotes	Prx1
T6069	23412-23413	HYPH	denotes	:
T6070	23413-23414	HYPH	denotes	:
T6068	23414-23417	NN	denotes	cre
T6071	23417-23418	-RRB-	denotes	)
T6072	23418-23420	,	denotes	,
T6073	23420-23424	DT	denotes	this
T6047	23425-23427	VBZ	denotes	is
T6074	23428-23431	RB	denotes	not
T6075	23432-23433	DT	denotes	a
T6076	23434-23443	NN	denotes	phenotype
T6077	23444-23454	JJ	denotes	consistent
T6078	23455-23459	IN	denotes	with
T6079	23460-23461	DT	denotes	a
T6080	23462-23470	NN	denotes	decrease
T6081	23471-23473	CC	denotes	or
T6082	23474-23478	NN	denotes	loss
T6083	23479-23481	IN	denotes	of
T6084	23482-23483	DT	denotes	a
T6086	23484-23495	RB	denotes	posteriorly
T6087	23496-23503	VBN	denotes	derived
T6088	23504-23514	NN	denotes	polarizing
T6085	23515-23523	NN	denotes	activity
T6089	23523-23524	.	denotes	.
T6090	23524-23671	sentence	denotes	Indeed, the terminal phalanx forms at the distal tip of each digit by a distinct mechanism, not dependent upon anterior-posterior patterning [38].
T6091	23525-23531	RB	denotes	Indeed
T6093	23531-23533	,	denotes	,
T6094	23533-23536	DT	denotes	the
T6096	23537-23545	JJ	denotes	terminal
T6095	23546-23553	NN	denotes	phalanx
T6092	23554-23559	VBZ	denotes	forms
T6097	23560-23562	IN	denotes	at
T6098	23563-23566	DT	denotes	the
T6100	23567-23573	JJ	denotes	distal
T6099	23574-23577	NN	denotes	tip
T6101	23578-23580	IN	denotes	of
T6102	23581-23585	DT	denotes	each
T6103	23586-23591	NN	denotes	digit
T6104	23592-23594	IN	denotes	by
T6105	23595-23596	DT	denotes	a
T6107	23597-23605	JJ	denotes	distinct
T6106	23606-23615	NN	denotes	mechanism
T6108	23615-23617	,	denotes	,
T6109	23617-23620	RB	denotes	not
T6110	23621-23630	JJ	denotes	dependent
T6111	23631-23635	IN	denotes	upon
T6112	23636-23644	JJ	denotes	anterior
T6114	23644-23645	HYPH	denotes	-
T6113	23645-23654	JJ	denotes	posterior
T6115	23655-23665	NN	denotes	patterning
T6116	23666-23667	-LRB-	denotes	[
T6117	23667-23669	CD	denotes	38
T6118	23669-23670	-RRB-	denotes	]
T6119	23670-23671	.	denotes	.
T6120	23671-23833	sentence	denotes	These data strongly indicate that BMP2 and BMP7 are not required secondary signals for SHH-mediated polarization of the digits along the anterior-posterior axis.
T6121	23672-23677	DT	denotes	These
T6122	23678-23682	NNS	denotes	data
T6124	23683-23691	RB	denotes	strongly
T6123	23692-23700	VBP	denotes	indicate
T6125	23701-23705	IN	denotes	that
T6127	23706-23710	NN	denotes	BMP2
T6128	23711-23714	CC	denotes	and
T6129	23715-23719	NN	denotes	BMP7
T6126	23720-23723	VBP	denotes	are
T6130	23724-23727	RB	denotes	not
T6131	23728-23736	VBN	denotes	required
T6133	23737-23746	JJ	denotes	secondary
T6132	23747-23754	NNS	denotes	signals
T6134	23755-23758	IN	denotes	for
T6135	23759-23762	NN	denotes	SHH
T6137	23762-23763	HYPH	denotes	-
T6136	23763-23771	VBN	denotes	mediated
T6138	23772-23784	NN	denotes	polarization
T6139	23785-23787	IN	denotes	of
T6140	23788-23791	DT	denotes	the
T6141	23792-23798	NNS	denotes	digits
T6142	23799-23804	IN	denotes	along
T6143	23805-23808	DT	denotes	the
T6145	23809-23817	JJ	denotes	anterior
T6147	23817-23818	HYPH	denotes	-
T6146	23818-23827	JJ	denotes	posterior
T6144	23828-23832	NN	denotes	axis
T6148	23832-23833	.	denotes	.
T6149	23833-24884	sentence	denotes	Figure 3 Patterning Defects in Limbs Deficient of Different Combinations of BMP Molecules (A–D) Bmp4 expression in limb buds from E11.5 wild-type (A and C) and Bmp2C/C, Bmp7 −/−; Prx1::cre (B and D) mouse embryos. (A and B) Forelimbs, (C and D) hindlimbs. (E–H) Shh (E and F) and Fgf8 (G and H) expression in E11.5 forelimbs and hindlimbs, respectively. (E and G) Wild-type embryos, (F and H) Bmp2C/C; Bmp4C/C; Prx1::cre embryos. (I–P) Sox9 (I–K) and Msx2 (M–O) expression in E12.5 wild-type (I and M) and Bmp2C/C; Bmp4C/C; Prx1::cre mouse embryonic forelimbs (J and N) and hindlimbs (K and O). (L and P) Shh expression in E12.5 wild-type (L) and Bmp2C/C; Bmp4C/C; Prx1::cre (P) embryonic hindlimbs. The red brackets in (F) and (H) show the broadened domains of expressions of Shh and Fgf8, respectively. The lack of transformations in digit identity in the limb skeletons formed in the absence of BMP2 and BMP7 activity also argues against BMP signaling being the critical instructive signal from the interdigital mesenchyme to the forming digits.
T6150	24641-24644	DT	denotes	The
T6151	24645-24649	NN	denotes	lack
T6153	24650-24652	IN	denotes	of
T6154	24653-24668	NNS	denotes	transformations
T6155	24669-24671	IN	denotes	in
T6156	24672-24677	NN	denotes	digit
T6157	24678-24686	NN	denotes	identity
T6158	24687-24689	IN	denotes	in
T6159	24690-24693	DT	denotes	the
T6161	24694-24698	NN	denotes	limb
T6160	24699-24708	NNS	denotes	skeletons
T6162	24709-24715	VBN	denotes	formed
T6163	24716-24718	IN	denotes	in
T6164	24719-24722	DT	denotes	the
T6165	24723-24730	NN	denotes	absence
T6166	24731-24733	IN	denotes	of
T6167	24734-24738	NN	denotes	BMP2
T6169	24739-24742	CC	denotes	and
T6170	24743-24747	NN	denotes	BMP7
T6168	24748-24756	NN	denotes	activity
T6171	24757-24761	RB	denotes	also
T6152	24762-24768	VBZ	denotes	argues
T6172	24769-24776	IN	denotes	against
T6173	24777-24780	NN	denotes	BMP
T6174	24781-24790	NN	denotes	signaling
T6175	24791-24796	VBG	denotes	being
T6176	24797-24800	DT	denotes	the
T6178	24801-24809	JJ	denotes	critical
T6179	24810-24821	JJ	denotes	instructive
T6177	24822-24828	NN	denotes	signal
T6180	24829-24833	IN	denotes	from
T6181	24834-24837	DT	denotes	the
T6183	24838-24850	JJ	denotes	interdigital
T6182	24851-24861	NN	denotes	mesenchyme
T6184	24862-24864	IN	denotes	to
T6185	24865-24868	DT	denotes	the
T6187	24869-24876	VBG	denotes	forming
T6186	24877-24883	NNS	denotes	digits
T6188	24883-24884	.	denotes	.
T6189	24884-25094	sentence	denotes	While a distal phalanx was lost from the middle digit of the forelimbs of these animals, these digits do not otherwise have a morphology consistent with their being transformed anteriorly into digit 1 (thumb).
T6190	24885-24890	IN	denotes	While
T6192	24891-24892	DT	denotes	a
T6194	24893-24899	JJ	denotes	distal
T6193	24900-24907	NN	denotes	phalanx
T6195	24908-24911	VBD	denotes	was
T6191	24912-24916	VBN	denotes	lost
T6197	24917-24921	IN	denotes	from
T6198	24922-24925	DT	denotes	the
T6200	24926-24932	JJ	denotes	middle
T6199	24933-24938	NN	denotes	digit
T6201	24939-24941	IN	denotes	of
T6202	24942-24945	DT	denotes	the
T6203	24946-24955	NNS	denotes	forelimbs
T6204	24956-24958	IN	denotes	of
T6205	24959-24964	DT	denotes	these
T6206	24965-24972	NNS	denotes	animals
T6207	24972-24974	,	denotes	,
T6208	24974-24979	DT	denotes	these
T6209	24980-24986	NNS	denotes	digits
T6210	24987-24989	VBP	denotes	do
T6211	24990-24993	RB	denotes	not
T6212	24994-25003	RB	denotes	otherwise
T6196	25004-25008	VB	denotes	have
T6213	25009-25010	DT	denotes	a
T6214	25011-25021	NN	denotes	morphology
T6215	25022-25032	JJ	denotes	consistent
T6216	25033-25037	IN	denotes	with
T6217	25038-25043	PRP$	denotes	their
T6219	25044-25049	VBG	denotes	being
T6218	25050-25061	VBN	denotes	transformed
T6220	25062-25072	RB	denotes	anteriorly
T6221	25073-25077	IN	denotes	into
T6222	25078-25083	NN	denotes	digit
T6223	25084-25085	CD	denotes	1
T6224	25086-25087	-LRB-	denotes	(
T6225	25087-25092	NN	denotes	thumb
T6226	25092-25093	-RRB-	denotes	)
T6227	25093-25094	.	denotes	.
T6228	25094-25314	sentence	denotes	Indeed, in the hindlimbs, where the penetrance of the phenotype is variable, the mutant middle digits are otherwise indistinguishable from wild-type, other than the presence or absence of this variable skeletal element.
T6229	25095-25101	RB	denotes	Indeed
T6231	25101-25103	,	denotes	,
T6232	25103-25105	IN	denotes	in
T6233	25106-25109	DT	denotes	the
T6234	25110-25119	NNS	denotes	hindlimbs
T6235	25119-25121	,	denotes	,
T6236	25121-25126	WRB	denotes	where
T6238	25127-25130	DT	denotes	the
T6239	25131-25141	NN	denotes	penetrance
T6240	25142-25144	IN	denotes	of
T6241	25145-25148	DT	denotes	the
T6242	25149-25158	NN	denotes	phenotype
T6237	25159-25161	VBZ	denotes	is
T6243	25162-25170	JJ	denotes	variable
T6244	25170-25172	,	denotes	,
T6245	25172-25175	DT	denotes	the
T6247	25176-25182	NN	denotes	mutant
T6248	25183-25189	JJ	denotes	middle
T6246	25190-25196	NNS	denotes	digits
T6230	25197-25200	VBP	denotes	are
T6249	25201-25210	RB	denotes	otherwise
T6250	25211-25228	JJ	denotes	indistinguishable
T6251	25229-25233	IN	denotes	from
T6252	25234-25238	JJ	denotes	wild
T6254	25238-25239	HYPH	denotes	-
T6253	25239-25243	NN	denotes	type
T6255	25243-25245	,	denotes	,
T6256	25245-25250	RB	denotes	other
T6257	25251-25255	IN	denotes	than
T6258	25256-25259	DT	denotes	the
T6259	25260-25268	NN	denotes	presence
T6260	25269-25271	CC	denotes	or
T6261	25272-25279	NN	denotes	absence
T6262	25280-25282	IN	denotes	of
T6263	25283-25287	DT	denotes	this
T6265	25288-25296	JJ	denotes	variable
T6266	25297-25305	JJ	denotes	skeletal
T6264	25306-25313	NN	denotes	element
T6267	25313-25314	.	denotes	.
T6268	25314-25556	sentence	denotes	In contrast to the proposed roles for BMPs in anterior-posterior digit patterning, we obtained further support for the well-accepted idea that a threshold level of BMP signaling is necessary interdigitally for programmed cell death to occur.
T6269	25315-25317	IN	denotes	In
T6271	25318-25326	NN	denotes	contrast
T6272	25327-25329	IN	denotes	to
T6273	25330-25333	DT	denotes	the
T6275	25334-25342	VBN	denotes	proposed
T6274	25343-25348	NNS	denotes	roles
T6276	25349-25352	IN	denotes	for
T6277	25353-25357	NNS	denotes	BMPs
T6278	25358-25360	IN	denotes	in
T6279	25361-25369	JJ	denotes	anterior
T6281	25369-25370	HYPH	denotes	-
T6280	25370-25379	JJ	denotes	posterior
T6283	25380-25385	NN	denotes	digit
T6282	25386-25396	NN	denotes	patterning
T6284	25396-25398	,	denotes	,
T6285	25398-25400	PRP	denotes	we
T6270	25401-25409	VBD	denotes	obtained
T6286	25410-25417	JJ	denotes	further
T6287	25418-25425	NN	denotes	support
T6288	25426-25429	IN	denotes	for
T6289	25430-25433	DT	denotes	the
T6291	25434-25438	RB	denotes	well
T6293	25438-25439	HYPH	denotes	-
T6292	25439-25447	VBN	denotes	accepted
T6290	25448-25452	NN	denotes	idea
T6294	25453-25457	IN	denotes	that
T6296	25458-25459	DT	denotes	a
T6298	25460-25469	NN	denotes	threshold
T6297	25470-25475	NN	denotes	level
T6299	25476-25478	IN	denotes	of
T6300	25479-25482	NN	denotes	BMP
T6301	25483-25492	NN	denotes	signaling
T6295	25493-25495	VBZ	denotes	is
T6302	25496-25505	JJ	denotes	necessary
T6303	25506-25520	RB	denotes	interdigitally
T6304	25521-25524	IN	denotes	for
T6306	25525-25535	VBN	denotes	programmed
T6308	25536-25540	NN	denotes	cell
T6307	25541-25546	NN	denotes	death
T6309	25547-25549	TO	denotes	to
T6305	25550-25555	VB	denotes	occur
T6310	25555-25556	.	denotes	.
T6311	25556-25792	sentence	denotes	We observed syndactyly with variable penetrance in limbs developing without BMP2 activity (Figure 2B), and nearly complete soft tissue syndactyly of all digits in limbs deficient in both BMP2 and BMP4 (compare Figure 2C and Figure 2D).
T6312	25557-25559	PRP	denotes	We
T6313	25560-25568	VBD	denotes	observed
T6314	25569-25579	NN	denotes	syndactyly
T6315	25580-25584	IN	denotes	with
T6316	25585-25593	JJ	denotes	variable
T6317	25594-25604	NN	denotes	penetrance
T6318	25605-25607	IN	denotes	in
T6319	25608-25613	NNS	denotes	limbs
T6320	25614-25624	VBG	denotes	developing
T6321	25625-25632	IN	denotes	without
T6322	25633-25637	NN	denotes	BMP2
T6323	25638-25646	NN	denotes	activity
T6324	25647-25648	-LRB-	denotes	(
T6326	25648-25654	NN	denotes	Figure
T6325	25655-25657	NN	denotes	2B
T6327	25657-25658	-RRB-	denotes	)
T6328	25658-25660	,	denotes	,
T6329	25660-25663	CC	denotes	and
T6330	25664-25670	RB	denotes	nearly
T6331	25671-25679	JJ	denotes	complete
T6333	25680-25684	JJ	denotes	soft
T6334	25685-25691	NN	denotes	tissue
T6332	25692-25702	NN	denotes	syndactyly
T6335	25703-25705	IN	denotes	of
T6336	25706-25709	DT	denotes	all
T6337	25710-25716	NNS	denotes	digits
T6338	25717-25719	IN	denotes	in
T6339	25720-25725	NNS	denotes	limbs
T6340	25726-25735	JJ	denotes	deficient
T6341	25736-25738	IN	denotes	in
T6342	25739-25743	CC	denotes	both
T6343	25744-25748	NN	denotes	BMP2
T6344	25749-25752	CC	denotes	and
T6345	25753-25757	NN	denotes	BMP4
T6346	25758-25759	-LRB-	denotes	(
T6347	25759-25766	VB	denotes	compare
T6348	25767-25773	NN	denotes	Figure
T6349	25774-25776	NN	denotes	2C
T6350	25777-25780	CC	denotes	and
T6351	25781-25787	NN	denotes	Figure
T6352	25788-25790	NN	denotes	2D
T6353	25790-25791	-RRB-	denotes	)
T6354	25791-25792	.	denotes	.
T6355	25792-26068	sentence	denotes	The one dramatic patterning defect we observed in our BMP deficiency series was the loss of posterior digits in forelimbs deficient in both BMP2 and BMP4 (Figure 1K), also observed in earlier limb buds stained to show no expression of the condensation marker Sox9 (Figure 3).
T6356	25793-25796	DT	denotes	The
T6358	25797-25800	CD	denotes	one
T6359	25801-25809	JJ	denotes	dramatic
T6360	25810-25820	NN	denotes	patterning
T6357	25821-25827	NN	denotes	defect
T6362	25828-25830	PRP	denotes	we
T6363	25831-25839	VBD	denotes	observed
T6364	25840-25842	IN	denotes	in
T6365	25843-25846	PRP$	denotes	our
T6367	25847-25850	NN	denotes	BMP
T6368	25851-25861	NN	denotes	deficiency
T6366	25862-25868	NN	denotes	series
T6361	25869-25872	VBD	denotes	was
T6369	25873-25876	DT	denotes	the
T6370	25877-25881	NN	denotes	loss
T6371	25882-25884	IN	denotes	of
T6372	25885-25894	JJ	denotes	posterior
T6373	25895-25901	NNS	denotes	digits
T6374	25902-25904	IN	denotes	in
T6375	25905-25914	NNS	denotes	forelimbs
T6376	25915-25924	JJ	denotes	deficient
T6377	25925-25927	IN	denotes	in
T6378	25928-25932	CC	denotes	both
T6379	25933-25937	NN	denotes	BMP2
T6380	25938-25941	CC	denotes	and
T6381	25942-25946	NN	denotes	BMP4
T6382	25947-25948	-LRB-	denotes	(
T6384	25948-25954	NN	denotes	Figure
T6383	25955-25957	NN	denotes	1K
T6385	25957-25958	-RRB-	denotes	)
T6386	25958-25960	,	denotes	,
T6387	25960-25964	RB	denotes	also
T6388	25965-25973	VBN	denotes	observed
T6389	25974-25976	IN	denotes	in
T6390	25977-25984	JJR	denotes	earlier
T6392	25985-25989	NN	denotes	limb
T6391	25990-25994	NNS	denotes	buds
T6393	25995-26002	VBN	denotes	stained
T6394	26003-26005	TO	denotes	to
T6395	26006-26010	VB	denotes	show
T6396	26011-26013	DT	denotes	no
T6397	26014-26024	NN	denotes	expression
T6398	26025-26027	IN	denotes	of
T6399	26028-26031	DT	denotes	the
T6401	26032-26044	NN	denotes	condensation
T6402	26045-26051	NN	denotes	marker
T6400	26052-26056	NN	denotes	Sox9
T6403	26057-26058	-LRB-	denotes	(
T6404	26058-26064	NN	denotes	Figure
T6405	26065-26066	CD	denotes	3
T6406	26066-26067	-RRB-	denotes	)
T6407	26067-26068	.	denotes	.
T6408	26068-26175	sentence	denotes	To understand this phenotype, we analyzed limb buds at various stages using a series of molecular markers.
T6409	26069-26071	TO	denotes	To
T6410	26072-26082	VB	denotes	understand
T6412	26083-26087	DT	denotes	this
T6413	26088-26097	NN	denotes	phenotype
T6414	26097-26099	,	denotes	,
T6415	26099-26101	PRP	denotes	we
T6411	26102-26110	VBD	denotes	analyzed
T6416	26111-26115	NN	denotes	limb
T6417	26116-26120	NNS	denotes	buds
T6418	26121-26123	IN	denotes	at
T6419	26124-26131	JJ	denotes	various
T6420	26132-26138	NNS	denotes	stages
T6421	26139-26144	VBG	denotes	using
T6422	26145-26146	DT	denotes	a
T6423	26147-26153	NN	denotes	series
T6424	26154-26156	IN	denotes	of
T6425	26157-26166	JJ	denotes	molecular
T6426	26167-26174	NNS	denotes	markers
T6427	26174-26175	.	denotes	.
T6428	26175-26300	sentence	denotes	By E11.5, the Bmp2C/C; Bmp4C/C; Prx1::cre limb buds are noticeably broader, expanded in the posterior relative to wild-type.
T6429	26176-26178	IN	denotes	By
T6431	26179-26184	NN	denotes	E11.5
T6432	26184-26186	,	denotes	,
T6433	26186-26189	DT	denotes	the
T6435	26190-26195	NN	denotes	Bmp2C
T6437	26195-26196	HYPH	denotes	/
T6436	26196-26197	NN	denotes	C
T6438	26197-26198	:	denotes	;
T6439	26199-26204	NN	denotes	Bmp4C
T6441	26204-26205	HYPH	denotes	/
T6440	26205-26206	NN	denotes	C
T6442	26206-26207	:	denotes	;
T6443	26208-26212	NN	denotes	Prx1
T6445	26212-26213	HYPH	denotes	:
T6446	26213-26214	HYPH	denotes	:
T6444	26214-26217	NN	denotes	cre
T6447	26218-26222	NN	denotes	limb
T6434	26223-26227	NNS	denotes	buds
T6430	26228-26231	VBP	denotes	are
T6448	26232-26242	RB	denotes	noticeably
T6449	26243-26250	JJR	denotes	broader
T6451	26250-26252	,	denotes	,
T6450	26252-26260	JJ	denotes	expanded
T6452	26261-26263	IN	denotes	in
T6453	26264-26267	DT	denotes	the
T6454	26268-26277	JJ	denotes	posterior
T6455	26278-26286	JJ	denotes	relative
T6456	26287-26289	IN	denotes	to
T6457	26290-26294	JJ	denotes	wild
T6459	26294-26295	HYPH	denotes	-
T6458	26295-26299	NN	denotes	type
T6460	26299-26300	.	denotes	.
T6461	26300-26498	sentence	denotes	This correlates with an anterior expansion in Shh expression (Figure 3E and 3F, red bracket) and a concomitant expansion of the AER as visualized by Fgf8 expression (Figure 3G and 3H, red bracket).
T6462	26301-26305	DT	denotes	This
T6463	26306-26316	VBZ	denotes	correlates
T6464	26317-26321	IN	denotes	with
T6465	26322-26324	DT	denotes	an
T6467	26325-26333	JJ	denotes	anterior
T6466	26334-26343	NN	denotes	expansion
T6468	26344-26346	IN	denotes	in
T6469	26347-26350	NN	denotes	Shh
T6470	26351-26361	NN	denotes	expression
T6471	26362-26363	-LRB-	denotes	(
T6473	26363-26369	NN	denotes	Figure
T6474	26370-26372	NN	denotes	3E
T6475	26373-26376	CC	denotes	and
T6476	26377-26379	NN	denotes	3F
T6477	26379-26381	,	denotes	,
T6478	26381-26384	JJ	denotes	red
T6472	26385-26392	NN	denotes	bracket
T6479	26392-26393	-RRB-	denotes	)
T6480	26394-26397	CC	denotes	and
T6481	26398-26399	DT	denotes	a
T6483	26400-26411	JJ	denotes	concomitant
T6482	26412-26421	NN	denotes	expansion
T6484	26422-26424	IN	denotes	of
T6485	26425-26428	DT	denotes	the
T6486	26429-26432	NN	denotes	AER
T6487	26433-26435	IN	denotes	as
T6488	26436-26446	VBN	denotes	visualized
T6489	26447-26449	IN	denotes	by
T6490	26450-26454	NN	denotes	Fgf8
T6491	26455-26465	NN	denotes	expression
T6492	26466-26467	-LRB-	denotes	(
T6494	26467-26473	NN	denotes	Figure
T6495	26474-26476	NN	denotes	3G
T6496	26477-26480	CC	denotes	and
T6497	26481-26483	NN	denotes	3H
T6498	26483-26485	,	denotes	,
T6499	26485-26488	JJ	denotes	red
T6493	26489-26496	NN	denotes	bracket
T6500	26496-26497	-RRB-	denotes	)
T6501	26497-26498	.	denotes	.
T6502	26498-26679	sentence	denotes	The expansion in Shh expression can first be detected at E10.5 and persists at least until E12.5, well after the time normal Shh expression disappears at E11.75 (Figure 3L and 3P).
T6503	26499-26502	DT	denotes	The
T6504	26503-26512	NN	denotes	expansion
T6506	26513-26515	IN	denotes	in
T6507	26516-26519	NN	denotes	Shh
T6508	26520-26530	NN	denotes	expression
T6509	26531-26534	MD	denotes	can
T6510	26535-26540	RB	denotes	first
T6511	26541-26543	VB	denotes	be
T6505	26544-26552	VBN	denotes	detected
T6512	26553-26555	IN	denotes	at
T6513	26556-26561	NN	denotes	E10.5
T6514	26562-26565	CC	denotes	and
T6515	26566-26574	VBZ	denotes	persists
T6516	26575-26577	RB	denotes	at
T6517	26578-26583	RBS	denotes	least
T6518	26584-26589	IN	denotes	until
T6519	26590-26595	NN	denotes	E12.5
T6520	26595-26597	,	denotes	,
T6521	26597-26601	RB	denotes	well
T6522	26602-26607	IN	denotes	after
T6523	26608-26611	DT	denotes	the
T6524	26612-26616	NN	denotes	time
T6525	26617-26623	JJ	denotes	normal
T6527	26624-26627	NN	denotes	Shh
T6526	26628-26638	NN	denotes	expression
T6528	26639-26649	VBZ	denotes	disappears
T6529	26650-26652	IN	denotes	at
T6530	26653-26659	NN	denotes	E11.75
T6531	26660-26661	-LRB-	denotes	(
T6533	26661-26667	NN	denotes	Figure
T6532	26668-26670	NN	denotes	3L
T6534	26671-26674	CC	denotes	and
T6535	26675-26677	NN	denotes	3P
T6536	26677-26678	-RRB-	denotes	)
T6537	26678-26679	.	denotes	.
T6538	26679-26847	sentence	denotes	The expansion of the AER in the absence of BMP signaling may be understood on the basis of the feedback loop between the zone of polarizing activity (ZPA) and the AER.
T6539	26680-26683	DT	denotes	The
T6540	26684-26693	NN	denotes	expansion
T6542	26694-26696	IN	denotes	of
T6543	26697-26700	DT	denotes	the
T6544	26701-26704	NN	denotes	AER
T6545	26705-26707	IN	denotes	in
T6546	26708-26711	DT	denotes	the
T6547	26712-26719	NN	denotes	absence
T6548	26720-26722	IN	denotes	of
T6549	26723-26726	NN	denotes	BMP
T6550	26727-26736	NN	denotes	signaling
T6551	26737-26740	MD	denotes	may
T6552	26741-26743	VB	denotes	be
T6541	26744-26754	VBN	denotes	understood
T6553	26755-26757	IN	denotes	on
T6554	26758-26761	DT	denotes	the
T6555	26762-26767	NN	denotes	basis
T6556	26768-26770	IN	denotes	of
T6557	26771-26774	DT	denotes	the
T6559	26775-26783	NN	denotes	feedback
T6558	26784-26788	NN	denotes	loop
T6560	26789-26796	IN	denotes	between
T6561	26797-26800	DT	denotes	the
T6562	26801-26805	NN	denotes	zone
T6563	26806-26808	IN	denotes	of
T6564	26809-26819	NN	denotes	polarizing
T6565	26820-26828	NN	denotes	activity
T6566	26829-26830	-LRB-	denotes	(
T6567	26830-26833	NN	denotes	ZPA
T6568	26833-26834	-RRB-	denotes	)
T6569	26835-26838	CC	denotes	and
T6570	26839-26842	DT	denotes	the
T6571	26843-26846	NN	denotes	AER
T6572	26846-26847	.	denotes	.
T6573	26847-26976	sentence	denotes	Expression of several Fgf genes [20,21] and maintenance of the AER itself [23] normally depend on BMP antagonism induced by SHH.
T6574	26848-26858	NN	denotes	Expression
T6576	26859-26861	IN	denotes	of
T6577	26862-26869	JJ	denotes	several
T6579	26870-26873	NN	denotes	Fgf
T6578	26874-26879	NNS	denotes	genes
T6580	26880-26881	-LRB-	denotes	[
T6582	26881-26883	CD	denotes	20
T6583	26883-26884	,	denotes	,
T6581	26884-26886	CD	denotes	21
T6584	26886-26887	-RRB-	denotes	]
T6585	26888-26891	CC	denotes	and
T6586	26892-26903	NN	denotes	maintenance
T6587	26904-26906	IN	denotes	of
T6588	26907-26910	DT	denotes	the
T6589	26911-26914	NN	denotes	AER
T6590	26915-26921	PRP	denotes	itself
T6591	26922-26923	-LRB-	denotes	[
T6592	26923-26925	CD	denotes	23
T6593	26925-26926	-RRB-	denotes	]
T6594	26927-26935	RB	denotes	normally
T6575	26936-26942	VBP	denotes	depend
T6595	26943-26945	IN	denotes	on
T6596	26946-26949	NN	denotes	BMP
T6597	26950-26960	NN	denotes	antagonism
T6598	26961-26968	VBN	denotes	induced
T6599	26969-26971	IN	denotes	by
T6600	26972-26975	NN	denotes	SHH
T6601	26975-26976	.	denotes	.
T6602	26976-27041	sentence	denotes	Thus, a decrease in BMP signaling expands and maintains the AER.
T6603	26977-26981	RB	denotes	Thus
T6605	26981-26983	,	denotes	,
T6606	26983-26984	DT	denotes	a
T6607	26985-26993	NN	denotes	decrease
T6608	26994-26996	IN	denotes	in
T6609	26997-27000	NN	denotes	BMP
T6610	27001-27010	NN	denotes	signaling
T6604	27011-27018	VBZ	denotes	expands
T6611	27019-27022	CC	denotes	and
T6612	27023-27032	VBZ	denotes	maintains
T6613	27033-27036	DT	denotes	the
T6614	27037-27040	NN	denotes	AER
T6615	27040-27041	.	denotes	.
T6616	27041-27194	sentence	denotes	Because Shh expression is, reciprocally, supported by FGF signaling from the AER [8,24], this, in turn, feeds back on the ZPA, expanding Shh expression.
T6617	27042-27049	IN	denotes	Because
T6619	27050-27053	NN	denotes	Shh
T6620	27054-27064	NN	denotes	expression
T6621	27065-27067	VBZ	denotes	is
T6622	27067-27069	,	denotes	,
T6623	27069-27081	RB	denotes	reciprocally
T6624	27081-27083	,	denotes	,
T6618	27083-27092	VBN	denotes	supported
T6626	27093-27095	IN	denotes	by
T6627	27096-27099	NN	denotes	FGF
T6628	27100-27109	NN	denotes	signaling
T6629	27110-27114	IN	denotes	from
T6630	27115-27118	DT	denotes	the
T6631	27119-27122	NN	denotes	AER
T6632	27123-27124	-LRB-	denotes	[
T6634	27124-27125	CD	denotes	8
T6635	27125-27126	,	denotes	,
T6633	27126-27128	CD	denotes	24
T6636	27128-27129	-RRB-	denotes	]
T6637	27129-27131	,	denotes	,
T6638	27131-27135	DT	denotes	this
T6639	27135-27137	,	denotes	,
T6640	27137-27139	IN	denotes	in
T6641	27140-27144	NN	denotes	turn
T6642	27144-27146	,	denotes	,
T6625	27146-27151	VBZ	denotes	feeds
T6643	27152-27156	RP	denotes	back
T6644	27157-27159	IN	denotes	on
T6645	27160-27163	DT	denotes	the
T6646	27164-27167	NN	denotes	ZPA
T6647	27167-27169	,	denotes	,
T6648	27169-27178	VBG	denotes	expanding
T6649	27179-27182	NN	denotes	Shh
T6650	27183-27193	NN	denotes	expression
T6651	27193-27194	.	denotes	.
T6652	27194-27563	sentence	denotes	While this explains the broader hand plate of the BMP2, BMP4–deficient limb buds, it is somewhat paradoxical in terms of the skeletal phenotype, as a broadened autopod would normally be expected to result in the formation of extra digits (polydactyly) (e.g., Figure 1G and 1O of this paper and [16]), not the loss of digits, as observed in the double mutant forelimbs.
T6653	27195-27200	IN	denotes	While
T6655	27201-27205	DT	denotes	this
T6654	27206-27214	VBZ	denotes	explains
T6657	27215-27218	DT	denotes	the
T6659	27219-27226	JJR	denotes	broader
T6660	27227-27231	NN	denotes	hand
T6658	27232-27237	NN	denotes	plate
T6661	27238-27240	IN	denotes	of
T6662	27241-27244	DT	denotes	the
T6664	27245-27249	NN	denotes	BMP2
T6666	27249-27251	,	denotes	,
T6665	27251-27255	NN	denotes	BMP4
T6668	27255-27256	HYPH	denotes	–
T6667	27256-27265	JJ	denotes	deficient
T6669	27266-27270	NN	denotes	limb
T6663	27271-27275	NNS	denotes	buds
T6670	27275-27277	,	denotes	,
T6671	27277-27279	PRP	denotes	it
T6656	27280-27282	VBZ	denotes	is
T6672	27283-27291	RB	denotes	somewhat
T6673	27292-27303	JJ	denotes	paradoxical
T6674	27304-27306	IN	denotes	in
T6675	27307-27312	NNS	denotes	terms
T6676	27313-27315	IN	denotes	of
T6677	27316-27319	DT	denotes	the
T6679	27320-27328	JJ	denotes	skeletal
T6678	27329-27338	NN	denotes	phenotype
T6680	27338-27340	,	denotes	,
T6681	27340-27342	IN	denotes	as
T6683	27343-27344	DT	denotes	a
T6685	27345-27354	VBN	denotes	broadened
T6684	27355-27362	NN	denotes	autopod
T6686	27363-27368	MD	denotes	would
T6687	27369-27377	RB	denotes	normally
T6688	27378-27380	VB	denotes	be
T6682	27381-27389	VBN	denotes	expected
T6689	27390-27392	TO	denotes	to
T6690	27393-27399	VB	denotes	result
T6691	27400-27402	IN	denotes	in
T6692	27403-27406	DT	denotes	the
T6693	27407-27416	NN	denotes	formation
T6694	27417-27419	IN	denotes	of
T6695	27420-27425	JJ	denotes	extra
T6696	27426-27432	NNS	denotes	digits
T6697	27433-27434	-LRB-	denotes	(
T6698	27434-27445	NN	denotes	polydactyly
T6699	27445-27446	-RRB-	denotes	)
T6700	27447-27448	-LRB-	denotes	(
T6702	27448-27452	FW	denotes	e.g.
T6703	27452-27454	,	denotes	,
T6704	27454-27460	NN	denotes	Figure
T6701	27461-27463	NN	denotes	1G
T6705	27464-27467	CC	denotes	and
T6706	27468-27470	NN	denotes	1O
T6707	27471-27473	IN	denotes	of
T6708	27474-27478	DT	denotes	this
T6709	27479-27484	NN	denotes	paper
T6710	27485-27488	CC	denotes	and
T6711	27489-27490	-LRB-	denotes	[
T6712	27490-27492	CD	denotes	16
T6713	27492-27493	-RRB-	denotes	]
T6714	27493-27494	-RRB-	denotes	)
T6715	27494-27496	,	denotes	,
T6716	27496-27499	CC	denotes	not
T6717	27500-27503	DT	denotes	the
T6718	27504-27508	NN	denotes	loss
T6719	27509-27511	IN	denotes	of
T6720	27512-27518	NNS	denotes	digits
T6721	27518-27520	,	denotes	,
T6722	27520-27522	IN	denotes	as
T6723	27523-27531	VBN	denotes	observed
T6724	27532-27534	IN	denotes	in
T6725	27535-27538	DT	denotes	the
T6727	27539-27545	JJ	denotes	double
T6728	27546-27552	NN	denotes	mutant
T6726	27553-27562	NNS	denotes	forelimbs
T6729	27562-27563	.	denotes	.
T6730	27563-27716	sentence	denotes	To examine the loss of digits more closely, BMP2, BMP4–deficient limb buds were examined during cartilage condensation phase of the digit rays at E12.5.
T6731	27564-27566	TO	denotes	To
T6732	27567-27574	VB	denotes	examine
T6734	27575-27578	DT	denotes	the
T6735	27579-27583	NN	denotes	loss
T6736	27584-27586	IN	denotes	of
T6737	27587-27593	NNS	denotes	digits
T6738	27594-27598	RBR	denotes	more
T6739	27599-27606	RB	denotes	closely
T6740	27606-27608	,	denotes	,
T6741	27608-27612	NN	denotes	BMP2
T6743	27612-27614	,	denotes	,
T6742	27614-27618	NN	denotes	BMP4
T6745	27618-27619	HYPH	denotes	–
T6744	27619-27628	JJ	denotes	deficient
T6747	27629-27633	NN	denotes	limb
T6746	27634-27638	NNS	denotes	buds
T6748	27639-27643	VBD	denotes	were
T6733	27644-27652	VBN	denotes	examined
T6749	27653-27659	IN	denotes	during
T6750	27660-27669	NN	denotes	cartilage
T6752	27670-27682	NN	denotes	condensation
T6751	27683-27688	NN	denotes	phase
T6753	27689-27691	IN	denotes	of
T6754	27692-27695	DT	denotes	the
T6756	27696-27701	NN	denotes	digit
T6755	27702-27706	NNS	denotes	rays
T6757	27707-27709	IN	denotes	at
T6758	27710-27715	NN	denotes	E12.5
T6759	27715-27716	.	denotes	.
T6760	27716-27920	sentence	denotes	In wild-type limb buds the condensation of all five digits can be visualized at this stage by in situ hybridization with a probe directed against Sox9, the earliest known chondrogenic marker (Figure 3I).
T6761	27717-27719	IN	denotes	In
T6763	27720-27724	JJ	denotes	wild
T6765	27724-27725	HYPH	denotes	-
T6764	27725-27729	NN	denotes	type
T6767	27730-27734	NN	denotes	limb
T6766	27735-27739	NNS	denotes	buds
T6768	27740-27743	DT	denotes	the
T6769	27744-27756	NN	denotes	condensation
T6770	27757-27759	IN	denotes	of
T6771	27760-27763	DT	denotes	all
T6773	27764-27768	CD	denotes	five
T6772	27769-27775	NNS	denotes	digits
T6774	27776-27779	MD	denotes	can
T6775	27780-27782	VB	denotes	be
T6762	27783-27793	VBN	denotes	visualized
T6776	27794-27796	IN	denotes	at
T6777	27797-27801	DT	denotes	this
T6778	27802-27807	NN	denotes	stage
T6779	27808-27810	IN	denotes	by
T6780	27811-27813	FW	denotes	in
T6781	27814-27818	FW	denotes	situ
T6782	27819-27832	NN	denotes	hybridization
T6783	27833-27837	IN	denotes	with
T6784	27838-27839	DT	denotes	a
T6785	27840-27845	NN	denotes	probe
T6786	27846-27854	VBN	denotes	directed
T6787	27855-27862	IN	denotes	against
T6788	27863-27867	NN	denotes	Sox9
T6789	27867-27869	,	denotes	,
T6790	27869-27872	DT	denotes	the
T6792	27873-27881	JJS	denotes	earliest
T6793	27882-27887	VBN	denotes	known
T6794	27888-27900	JJ	denotes	chondrogenic
T6791	27901-27907	NN	denotes	marker
T6795	27908-27909	-LRB-	denotes	(
T6797	27909-27915	NN	denotes	Figure
T6796	27916-27918	NN	denotes	3I
T6798	27918-27919	-RRB-	denotes	)
T6799	27919-27920	.	denotes	.
T6800	27920-28148	sentence	denotes	However, in Bmp2C/C; Bmp4C/C; Prx1::cre limb buds at E12.5, Sox9 only detects primordia of the anterior three digits of the forelimb, indicating a failure in the formation of posterior digits at this earliest stage (Figure 3J).
T6801	27921-27928	RB	denotes	However
T6803	27928-27930	,	denotes	,
T6804	27930-27932	IN	denotes	in
T6805	27933-27938	NN	denotes	Bmp2C
T6807	27938-27939	HYPH	denotes	/
T6806	27939-27940	NN	denotes	C
T6809	27940-27941	:	denotes	;
T6810	27942-27947	NN	denotes	Bmp4C
T6812	27947-27948	HYPH	denotes	/
T6811	27948-27949	NN	denotes	C
T6813	27949-27950	:	denotes	;
T6814	27951-27955	NN	denotes	Prx1
T6816	27955-27956	HYPH	denotes	:
T6817	27956-27957	HYPH	denotes	:
T6815	27957-27960	NN	denotes	cre
T6818	27961-27965	NN	denotes	limb
T6808	27966-27970	NNS	denotes	buds
T6819	27971-27973	IN	denotes	at
T6820	27974-27979	NN	denotes	E12.5
T6821	27979-27981	,	denotes	,
T6822	27981-27985	NN	denotes	Sox9
T6823	27986-27990	RB	denotes	only
T6802	27991-27998	VBZ	denotes	detects
T6824	27999-28008	NNS	denotes	primordia
T6825	28009-28011	IN	denotes	of
T6826	28012-28015	DT	denotes	the
T6828	28016-28024	JJ	denotes	anterior
T6829	28025-28030	CD	denotes	three
T6827	28031-28037	NNS	denotes	digits
T6830	28038-28040	IN	denotes	of
T6831	28041-28044	DT	denotes	the
T6832	28045-28053	NN	denotes	forelimb
T6833	28053-28055	,	denotes	,
T6834	28055-28065	VBG	denotes	indicating
T6835	28066-28067	DT	denotes	a
T6836	28068-28075	NN	denotes	failure
T6837	28076-28078	IN	denotes	in
T6838	28079-28082	DT	denotes	the
T6839	28083-28092	NN	denotes	formation
T6840	28093-28095	IN	denotes	of
T6841	28096-28105	JJ	denotes	posterior
T6842	28106-28112	NNS	denotes	digits
T6843	28113-28115	IN	denotes	at
T6844	28116-28120	DT	denotes	this
T6846	28121-28129	JJS	denotes	earliest
T6845	28130-28135	NN	denotes	stage
T6847	28136-28137	-LRB-	denotes	(
T6849	28137-28143	NN	denotes	Figure
T6848	28144-28146	NN	denotes	3J
T6850	28146-28147	-RRB-	denotes	)
T6851	28147-28148	.	denotes	.
T6852	28148-28359	sentence	denotes	Genes such as Msx2 (Figure 3N) continue to be expressed throughout the posterior forelimb mesenchyme, indicating that the posterior forelimb tissue is viable at E12.5 in the Bmp2C/C; Bmp4C/C; Prx1::cre animals.
T6853	28149-28154	NNS	denotes	Genes
T6855	28155-28159	JJ	denotes	such
T6856	28160-28162	IN	denotes	as
T6857	28163-28167	NN	denotes	Msx2
T6858	28168-28169	-LRB-	denotes	(
T6860	28169-28175	NN	denotes	Figure
T6859	28176-28178	NN	denotes	3N
T6861	28178-28179	-RRB-	denotes	)
T6854	28180-28188	VBP	denotes	continue
T6862	28189-28191	TO	denotes	to
T6864	28192-28194	VB	denotes	be
T6863	28195-28204	VBN	denotes	expressed
T6865	28205-28215	IN	denotes	throughout
T6866	28216-28219	DT	denotes	the
T6868	28220-28229	JJ	denotes	posterior
T6869	28230-28238	NN	denotes	forelimb
T6867	28239-28249	NN	denotes	mesenchyme
T6870	28249-28251	,	denotes	,
T6871	28251-28261	VBG	denotes	indicating
T6872	28262-28266	IN	denotes	that
T6874	28267-28270	DT	denotes	the
T6876	28271-28280	JJ	denotes	posterior
T6877	28281-28289	NN	denotes	forelimb
T6875	28290-28296	NN	denotes	tissue
T6873	28297-28299	VBZ	denotes	is
T6878	28300-28306	JJ	denotes	viable
T6879	28307-28309	IN	denotes	at
T6880	28310-28315	NN	denotes	E12.5
T6881	28316-28318	IN	denotes	in
T6882	28319-28322	DT	denotes	the
T6884	28323-28328	NN	denotes	Bmp2C
T6886	28328-28329	HYPH	denotes	/
T6885	28329-28330	NN	denotes	C
T6887	28330-28331	:	denotes	;
T6888	28332-28337	NN	denotes	Bmp4C
T6890	28337-28338	HYPH	denotes	/
T6889	28338-28339	NN	denotes	C
T6891	28339-28340	:	denotes	;
T6892	28341-28345	NN	denotes	Prx1
T6894	28345-28346	HYPH	denotes	:
T6895	28346-28347	HYPH	denotes	:
T6893	28347-28350	NN	denotes	cre
T6883	28351-28358	NNS	denotes	animals
T6896	28358-28359	.	denotes	.
T6897	28359-28542	sentence	denotes	Msx2 is normally expressed in the non-chondrogenic interdigital mesenchyme at this stage (Figure 3M), a continuation of an earlier expression domain throughout the distal mesenchyme.
T6898	28360-28364	NN	denotes	Msx2
T6900	28365-28367	VBZ	denotes	is
T6901	28368-28376	RB	denotes	normally
T6899	28377-28386	VBN	denotes	expressed
T6902	28387-28389	IN	denotes	in
T6903	28390-28393	DT	denotes	the
T6905	28394-28410	JJ	denotes	non-chondrogenic
T6906	28411-28423	JJ	denotes	interdigital
T6904	28424-28434	NN	denotes	mesenchyme
T6907	28435-28437	IN	denotes	at
T6908	28438-28442	DT	denotes	this
T6909	28443-28448	NN	denotes	stage
T6910	28449-28450	-LRB-	denotes	(
T6912	28450-28456	NN	denotes	Figure
T6911	28457-28459	NN	denotes	3M
T6913	28459-28460	-RRB-	denotes	)
T6914	28460-28462	,	denotes	,
T6915	28462-28463	DT	denotes	a
T6916	28464-28476	NN	denotes	continuation
T6917	28477-28479	IN	denotes	of
T6918	28480-28482	DT	denotes	an
T6920	28483-28490	JJR	denotes	earlier
T6921	28491-28501	NN	denotes	expression
T6919	28502-28508	NN	denotes	domain
T6922	28509-28519	IN	denotes	throughout
T6923	28520-28523	DT	denotes	the
T6925	28524-28530	JJ	denotes	distal
T6924	28531-28541	NN	denotes	mesenchyme
T6926	28541-28542	.	denotes	.
T6927	28542-28674	sentence	denotes	This expression of Msx2 in the posterior of the BMP2, BMP4–deficient forelimbs reflects a failure of chondrogenesis in this region.
T6928	28543-28547	DT	denotes	This
T6929	28548-28558	NN	denotes	expression
T6931	28559-28561	IN	denotes	of
T6932	28562-28566	NN	denotes	Msx2
T6933	28567-28569	IN	denotes	in
T6934	28570-28573	DT	denotes	the
T6935	28574-28583	NN	denotes	posterior
T6936	28584-28586	IN	denotes	of
T6937	28587-28590	DT	denotes	the
T6939	28591-28595	NN	denotes	BMP2
T6941	28595-28597	,	denotes	,
T6940	28597-28601	NN	denotes	BMP4
T6943	28601-28602	HYPH	denotes	–
T6942	28602-28611	JJ	denotes	deficient
T6938	28612-28621	NNS	denotes	forelimbs
T6930	28622-28630	VBZ	denotes	reflects
T6944	28631-28632	DT	denotes	a
T6945	28633-28640	NN	denotes	failure
T6946	28641-28643	IN	denotes	of
T6947	28644-28658	NN	denotes	chondrogenesis
T6948	28659-28661	IN	denotes	in
T6949	28662-28666	DT	denotes	this
T6950	28667-28673	NN	denotes	region
T6951	28673-28674	.	denotes	.
T6952	28674-28988	sentence	denotes	The loss of the posterior forelimb digits in these mice thus does not appear to represent a decrease in polarizing activity leading to a change in digit identity rather a consequence of the overall level of BMP signaling falling below a threshold for initiating chondrogenesis in the posterior of these forelimbs.
T6953	28675-28678	DT	denotes	The
T6954	28679-28683	NN	denotes	loss
T6956	28684-28686	IN	denotes	of
T6957	28687-28690	DT	denotes	the
T6959	28691-28700	JJ	denotes	posterior
T6960	28701-28709	NN	denotes	forelimb
T6958	28710-28716	NNS	denotes	digits
T6961	28717-28719	IN	denotes	in
T6962	28720-28725	DT	denotes	these
T6963	28726-28730	NNS	denotes	mice
T6964	28731-28735	RB	denotes	thus
T6965	28736-28740	VBZ	denotes	does
T6966	28741-28744	RB	denotes	not
T6955	28745-28751	VB	denotes	appear
T6967	28752-28754	TO	denotes	to
T6968	28755-28764	VB	denotes	represent
T6969	28765-28766	DT	denotes	a
T6970	28767-28775	NN	denotes	decrease
T6971	28776-28778	IN	denotes	in
T6972	28779-28789	VBG	denotes	polarizing
T6973	28790-28798	NN	denotes	activity
T6974	28799-28806	VBG	denotes	leading
T6975	28807-28809	IN	denotes	to
T6976	28810-28811	DT	denotes	a
T6977	28812-28818	NN	denotes	change
T6978	28819-28821	IN	denotes	in
T6979	28822-28827	NN	denotes	digit
T6980	28828-28836	NN	denotes	identity
T6981	28837-28843	RB	denotes	rather
T6983	28844-28845	DT	denotes	a
T6982	28846-28857	NN	denotes	consequence
T6984	28858-28860	IN	denotes	of
T6985	28861-28864	DT	denotes	the
T6987	28865-28872	JJ	denotes	overall
T6986	28873-28878	NN	denotes	level
T6989	28879-28881	IN	denotes	of
T6990	28882-28885	NN	denotes	BMP
T6991	28886-28895	NN	denotes	signaling
T6988	28896-28903	VBG	denotes	falling
T6992	28904-28909	IN	denotes	below
T6993	28910-28911	DT	denotes	a
T6994	28912-28921	NN	denotes	threshold
T6995	28922-28925	IN	denotes	for
T6996	28926-28936	VBG	denotes	initiating
T6997	28937-28951	NN	denotes	chondrogenesis
T6998	28952-28954	IN	denotes	in
T6999	28955-28958	DT	denotes	the
T7000	28959-28968	NN	denotes	posterior
T7001	28969-28971	IN	denotes	of
T7002	28972-28977	DT	denotes	these
T7003	28978-28987	NNS	denotes	forelimbs
T7004	28987-28988	.	denotes	.
T7005	28988-29246	sentence	denotes	The posterior of the hindlimb in the mutant, in contrast, appears to remain above this threshold, and more than five digit condensations form in the hindlimb bud (Figures 3K and 1S) and no ectopic expression of Msx2 is observed in the posterior (Figure 3O).
T7006	28989-28992	DT	denotes	The
T7007	28993-29002	JJ	denotes	posterior
T7009	29003-29005	IN	denotes	of
T7010	29006-29009	DT	denotes	the
T7011	29010-29018	NN	denotes	hindlimb
T7012	29019-29021	IN	denotes	in
T7013	29022-29025	DT	denotes	the
T7014	29026-29032	NN	denotes	mutant
T7015	29032-29034	,	denotes	,
T7016	29034-29036	IN	denotes	in
T7017	29037-29045	NN	denotes	contrast
T7018	29045-29047	,	denotes	,
T7008	29047-29054	VBZ	denotes	appears
T7019	29055-29057	TO	denotes	to
T7020	29058-29064	VB	denotes	remain
T7021	29065-29070	IN	denotes	above
T7022	29071-29075	DT	denotes	this
T7023	29076-29085	NN	denotes	threshold
T7024	29085-29087	,	denotes	,
T7025	29087-29090	CC	denotes	and
T7026	29091-29095	JJR	denotes	more
T7028	29096-29100	IN	denotes	than
T7027	29101-29105	CD	denotes	five
T7030	29106-29111	NN	denotes	digit
T7029	29112-29125	NNS	denotes	condensations
T7031	29126-29130	VBP	denotes	form
T7032	29131-29133	IN	denotes	in
T7033	29134-29137	DT	denotes	the
T7035	29138-29146	NN	denotes	hindlimb
T7034	29147-29150	NN	denotes	bud
T7036	29151-29152	-LRB-	denotes	(
T7038	29152-29159	NNS	denotes	Figures
T7037	29160-29162	NN	denotes	3K
T7039	29163-29166	CC	denotes	and
T7040	29167-29169	NN	denotes	1S
T7041	29169-29170	-RRB-	denotes	)
T7042	29171-29174	CC	denotes	and
T7043	29175-29177	DT	denotes	no
T7045	29178-29185	JJ	denotes	ectopic
T7044	29186-29196	NN	denotes	expression
T7047	29197-29199	IN	denotes	of
T7048	29200-29204	NN	denotes	Msx2
T7049	29205-29207	VBZ	denotes	is
T7046	29208-29216	VBN	denotes	observed
T7050	29217-29219	IN	denotes	in
T7051	29220-29223	DT	denotes	the
T7052	29224-29233	JJ	denotes	posterior
T7053	29234-29235	-LRB-	denotes	(
T7055	29235-29241	NN	denotes	Figure
T7054	29242-29244	NN	denotes	3O
T7056	29244-29245	-RRB-	denotes	)
T7057	29245-29246	.	denotes	.
T7182	29248-29256	JJ	denotes	Skeletal
T7183	29257-29272	NN	denotes	Differentiation
T7184	29273-29275	IN	denotes	in
T7185	29276-29281	NNS	denotes	Limbs
T7186	29282-29292	VBG	denotes	Developing
T7187	29293-29297	IN	denotes	with
T7188	29298-29305	VBN	denotes	Reduced
T7189	29306-29312	NNS	denotes	Levels
T7190	29313-29315	IN	denotes	of
T7191	29316-29319	NN	denotes	BMP
T7192	29320-29329	NN	denotes	Signaling
T7193	29329-29403	sentence	denotes	BMPs were first discovered in the context of their skeletogenic activity.
T7194	29330-29334	NNS	denotes	BMPs
T7196	29335-29339	VBD	denotes	were
T7197	29340-29345	RB	denotes	first
T7195	29346-29356	VBN	denotes	discovered
T7198	29357-29359	IN	denotes	in
T7199	29360-29363	DT	denotes	the
T7200	29364-29371	NN	denotes	context
T7201	29372-29374	IN	denotes	of
T7202	29375-29380	PRP$	denotes	their
T7204	29381-29393	JJ	denotes	skeletogenic
T7203	29394-29402	NN	denotes	activity
T7205	29402-29403	.	denotes	.
T7206	29403-29561	sentence	denotes	We therefore wanted to determine whether the loss of the specific BMP molecules under investigation here would perturb the skeletal differentiation pathways.
T7207	29404-29406	PRP	denotes	We
T7209	29407-29416	RB	denotes	therefore
T7208	29417-29423	VBD	denotes	wanted
T7210	29424-29426	TO	denotes	to
T7211	29427-29436	VB	denotes	determine
T7212	29437-29444	IN	denotes	whether
T7214	29445-29448	DT	denotes	the
T7215	29449-29453	NN	denotes	loss
T7216	29454-29456	IN	denotes	of
T7217	29457-29460	DT	denotes	the
T7219	29461-29469	JJ	denotes	specific
T7220	29470-29473	NN	denotes	BMP
T7218	29474-29483	NNS	denotes	molecules
T7221	29484-29489	IN	denotes	under
T7222	29490-29503	NN	denotes	investigation
T7223	29504-29508	RB	denotes	here
T7224	29509-29514	MD	denotes	would
T7213	29515-29522	VB	denotes	perturb
T7225	29523-29526	DT	denotes	the
T7227	29527-29535	JJ	denotes	skeletal
T7228	29536-29551	NN	denotes	differentiation
T7226	29552-29560	NNS	denotes	pathways
T7229	29560-29561	.	denotes	.
T7230	29561-29691	sentence	denotes	Accordingly, skeletal preparations from each of the various mutant alleles were subjected to histological and molecular analyses.
T7231	29562-29573	RB	denotes	Accordingly
T7233	29573-29575	,	denotes	,
T7234	29575-29583	JJ	denotes	skeletal
T7235	29584-29596	NNS	denotes	preparations
T7236	29597-29601	IN	denotes	from
T7237	29602-29606	DT	denotes	each
T7238	29607-29609	IN	denotes	of
T7239	29610-29613	DT	denotes	the
T7241	29614-29621	JJ	denotes	various
T7242	29622-29628	NN	denotes	mutant
T7240	29629-29636	NNS	denotes	alleles
T7243	29637-29641	VBD	denotes	were
T7232	29642-29651	VBN	denotes	subjected
T7244	29652-29654	IN	denotes	to
T7245	29655-29667	JJ	denotes	histological
T7247	29668-29671	CC	denotes	and
T7248	29672-29681	JJ	denotes	molecular
T7246	29682-29690	NNS	denotes	analyses
T7249	29690-29691	.	denotes	.
T7250	29691-29858	sentence	denotes	The skeletal elements that formed in the absence of BMP2, BMP4, or BMP7 or BMP2 and BMP7 all underwent normal chondrogenesis and osteogenesis by a series of criteria.
T7251	29692-29695	DT	denotes	The
T7253	29696-29704	JJ	denotes	skeletal
T7252	29705-29713	NNS	denotes	elements
T7255	29714-29718	WDT	denotes	that
T7256	29719-29725	VBD	denotes	formed
T7257	29726-29728	IN	denotes	in
T7258	29729-29732	DT	denotes	the
T7259	29733-29740	NN	denotes	absence
T7260	29741-29743	IN	denotes	of
T7261	29744-29748	NN	denotes	BMP2
T7262	29748-29750	,	denotes	,
T7263	29750-29754	NN	denotes	BMP4
T7264	29754-29756	,	denotes	,
T7265	29756-29758	CC	denotes	or
T7266	29759-29763	NN	denotes	BMP7
T7267	29764-29766	CC	denotes	or
T7268	29767-29771	NN	denotes	BMP2
T7269	29772-29775	CC	denotes	and
T7270	29776-29780	NN	denotes	BMP7
T7271	29781-29784	DT	denotes	all
T7254	29785-29794	VBD	denotes	underwent
T7272	29795-29801	JJ	denotes	normal
T7273	29802-29816	NN	denotes	chondrogenesis
T7274	29817-29820	CC	denotes	and
T7275	29821-29833	NN	denotes	osteogenesis
T7276	29834-29836	IN	denotes	by
T7277	29837-29838	DT	denotes	a
T7278	29839-29845	NN	denotes	series
T7279	29846-29848	IN	denotes	of
T7280	29849-29857	NNS	denotes	criteria
T7281	29857-29858	.	denotes	.
T7282	29858-29975	sentence	denotes	All showed normal staining with Alcian blue (for cartilage) and Alizarin red (for mineralized tissue, see Figure 1).
T7283	29859-29862	DT	denotes	All
T7284	29863-29869	VBD	denotes	showed
T7285	29870-29876	JJ	denotes	normal
T7286	29877-29885	NN	denotes	staining
T7287	29886-29890	IN	denotes	with
T7288	29891-29897	JJ	denotes	Alcian
T7289	29898-29902	JJ	denotes	blue
T7290	29903-29904	-LRB-	denotes	(
T7291	29904-29907	IN	denotes	for
T7292	29908-29917	NN	denotes	cartilage
T7293	29917-29918	-RRB-	denotes	)
T7294	29919-29922	CC	denotes	and
T7295	29923-29931	JJ	denotes	Alizarin
T7296	29932-29935	JJ	denotes	red
T7297	29936-29937	-LRB-	denotes	(
T7299	29937-29940	IN	denotes	for
T7300	29941-29952	VBN	denotes	mineralized
T7301	29953-29959	NN	denotes	tissue
T7302	29959-29961	,	denotes	,
T7298	29961-29964	VBP	denotes	see
T7303	29965-29971	NN	denotes	Figure
T7304	29972-29973	CD	denotes	1
T7305	29973-29974	-RRB-	denotes	)
T7306	29974-29975	.	denotes	.
T7307	29975-30160	sentence	denotes	Most were also analyzed in histological sections stained with toluidine blue and showed osteoid formation and bone matrix deposition (compare Figure S2A and S2E; and unpublished data).
T7308	29976-29980	JJS	denotes	Most
T7310	29981-29985	VBD	denotes	were
T7311	29986-29990	RB	denotes	also
T7309	29991-29999	VBN	denotes	analyzed
T7312	30000-30002	IN	denotes	in
T7313	30003-30015	JJ	denotes	histological
T7314	30016-30024	NNS	denotes	sections
T7315	30025-30032	VBN	denotes	stained
T7316	30033-30037	IN	denotes	with
T7317	30038-30047	NN	denotes	toluidine
T7318	30048-30052	JJ	denotes	blue
T7319	30053-30056	CC	denotes	and
T7320	30057-30063	VBD	denotes	showed
T7321	30064-30071	JJ	denotes	osteoid
T7322	30072-30081	NN	denotes	formation
T7323	30082-30085	CC	denotes	and
T7324	30086-30090	NN	denotes	bone
T7325	30091-30097	NN	denotes	matrix
T7326	30098-30108	NN	denotes	deposition
T7327	30109-30110	-LRB-	denotes	(
T7328	30110-30117	VB	denotes	compare
T7329	30118-30124	NN	denotes	Figure
T7330	30125-30128	NN	denotes	S2A
T7331	30129-30132	CC	denotes	and
T7332	30133-30136	NN	denotes	S2E
T7333	30136-30137	:	denotes	;
T7334	30138-30141	CC	denotes	and
T7335	30142-30153	JJ	denotes	unpublished
T7336	30154-30158	NNS	denotes	data
T7337	30158-30159	-RRB-	denotes	)
T7338	30159-30160	.	denotes	.
T7339	30160-30518	sentence	denotes	The skeletal elements in these various BMP-deficient animals contained normal prehypertrophic chondrocytes expressing type II collagen, hypertrophic chondrocytes expressing type X collagen, as well as the osteoblasts expressing type I collagen, like their wild-type counterparts (Figure S2; compare Figure S2F–S2H with Figure S2B–S2D; and unpublished data).
T7340	30161-30164	DT	denotes	The
T7342	30165-30173	JJ	denotes	skeletal
T7341	30174-30182	NNS	denotes	elements
T7344	30183-30185	IN	denotes	in
T7345	30186-30191	DT	denotes	these
T7347	30192-30199	JJ	denotes	various
T7348	30200-30203	NN	denotes	BMP
T7350	30203-30204	HYPH	denotes	-
T7349	30204-30213	JJ	denotes	deficient
T7346	30214-30221	NNS	denotes	animals
T7343	30222-30231	VBD	denotes	contained
T7351	30232-30238	JJ	denotes	normal
T7353	30239-30254	JJ	denotes	prehypertrophic
T7352	30255-30267	NNS	denotes	chondrocytes
T7354	30268-30278	VBG	denotes	expressing
T7355	30279-30283	NN	denotes	type
T7357	30284-30286	CD	denotes	II
T7356	30287-30295	NN	denotes	collagen
T7358	30295-30297	,	denotes	,
T7359	30297-30309	JJ	denotes	hypertrophic
T7360	30310-30322	NNS	denotes	chondrocytes
T7361	30323-30333	VBG	denotes	expressing
T7362	30334-30338	NN	denotes	type
T7364	30339-30340	CD	denotes	X
T7363	30341-30349	NN	denotes	collagen
T7365	30349-30351	,	denotes	,
T7366	30351-30353	RB	denotes	as
T7368	30354-30358	RB	denotes	well
T7367	30359-30361	IN	denotes	as
T7369	30362-30365	DT	denotes	the
T7370	30366-30377	NNS	denotes	osteoblasts
T7371	30378-30388	VBG	denotes	expressing
T7372	30389-30393	NN	denotes	type
T7374	30394-30395	CD	denotes	I
T7373	30396-30404	NN	denotes	collagen
T7375	30404-30406	,	denotes	,
T7376	30406-30410	IN	denotes	like
T7377	30411-30416	PRP$	denotes	their
T7379	30417-30421	JJ	denotes	wild
T7381	30421-30422	HYPH	denotes	-
T7380	30422-30426	NN	denotes	type
T7378	30427-30439	NNS	denotes	counterparts
T7382	30440-30441	-LRB-	denotes	(
T7384	30441-30447	NN	denotes	Figure
T7383	30448-30450	NN	denotes	S2
T7385	30450-30451	:	denotes	;
T7386	30452-30459	VB	denotes	compare
T7387	30460-30466	NN	denotes	Figure
T7388	30467-30470	NN	denotes	S2F
T7389	30470-30471	SYM	denotes	–
T7390	30471-30474	NN	denotes	S2H
T7391	30475-30479	IN	denotes	with
T7392	30480-30486	NN	denotes	Figure
T7393	30487-30490	NN	denotes	S2B
T7394	30490-30491	SYM	denotes	–
T7395	30491-30494	NN	denotes	S2D
T7396	30494-30495	:	denotes	;
T7397	30496-30499	CC	denotes	and
T7398	30500-30511	JJ	denotes	unpublished
T7399	30512-30516	NNS	denotes	data
T7400	30516-30517	-RRB-	denotes	)
T7401	30517-30518	.	denotes	.
T7402	30518-30649	sentence	denotes	In contrast, however, limbs completely deficient in both BMP2 and BMP4 activity showed severe defects in skeletal differentiation.
T7403	30519-30521	IN	denotes	In
T7405	30522-30530	NN	denotes	contrast
T7406	30530-30532	,	denotes	,
T7407	30532-30539	RB	denotes	however
T7408	30539-30541	,	denotes	,
T7409	30541-30546	NNS	denotes	limbs
T7410	30547-30557	RB	denotes	completely
T7411	30558-30567	JJ	denotes	deficient
T7412	30568-30570	IN	denotes	in
T7413	30571-30575	CC	denotes	both
T7414	30576-30580	NN	denotes	BMP2
T7416	30581-30584	CC	denotes	and
T7417	30585-30589	NN	denotes	BMP4
T7415	30590-30598	NN	denotes	activity
T7404	30599-30605	VBD	denotes	showed
T7418	30606-30612	JJ	denotes	severe
T7419	30613-30620	NNS	denotes	defects
T7420	30621-30623	IN	denotes	in
T7421	30624-30632	JJ	denotes	skeletal
T7422	30633-30648	NN	denotes	differentiation
T7423	30648-30649	.	denotes	.
T7592	30651-30655	NN	denotes	BMP2
T7594	30656-30659	CC	denotes	and
T7595	30660-30664	NN	denotes	BMP4
T7593	30665-30668	VBP	denotes	Are
T7596	30669-30672	RB	denotes	Not
T7597	30673-30681	JJ	denotes	Critical
T7598	30682-30685	IN	denotes	for
T7599	30686-30698	JJ	denotes	Chondrogenic
T7600	30699-30714	NN	denotes	Differentiation
T7601	30714-30885	sentence	denotes	As noted above, at E12.5, chondrogenic condensation of the digits could be visualized in Bmp2C/C; Bmp4C/C; Prx1::cre limb buds by in situ hybridization with a Sox9 probe.
T7602	30715-30717	IN	denotes	As
T7603	30718-30723	VBN	denotes	noted
T7605	30724-30729	RB	denotes	above
T7606	30729-30731	,	denotes	,
T7607	30731-30733	IN	denotes	at
T7608	30734-30739	NN	denotes	E12.5
T7609	30739-30741	,	denotes	,
T7610	30741-30753	JJ	denotes	chondrogenic
T7611	30754-30766	NN	denotes	condensation
T7612	30767-30769	IN	denotes	of
T7613	30770-30773	DT	denotes	the
T7614	30774-30780	NNS	denotes	digits
T7615	30781-30786	MD	denotes	could
T7616	30787-30789	VB	denotes	be
T7604	30790-30800	VBN	denotes	visualized
T7617	30801-30803	IN	denotes	in
T7618	30804-30809	NN	denotes	Bmp2C
T7620	30809-30810	HYPH	denotes	/
T7619	30810-30811	NN	denotes	C
T7622	30811-30812	:	denotes	;
T7623	30813-30818	NN	denotes	Bmp4C
T7625	30818-30819	HYPH	denotes	/
T7624	30819-30820	NN	denotes	C
T7626	30820-30821	:	denotes	;
T7627	30822-30826	NN	denotes	Prx1
T7629	30826-30827	HYPH	denotes	:
T7630	30827-30828	HYPH	denotes	:
T7628	30828-30831	NN	denotes	cre
T7631	30832-30836	NN	denotes	limb
T7621	30837-30841	NNS	denotes	buds
T7632	30842-30844	IN	denotes	by
T7633	30845-30847	FW	denotes	in
T7634	30848-30852	FW	denotes	situ
T7635	30853-30866	NN	denotes	hybridization
T7636	30867-30871	IN	denotes	with
T7637	30872-30873	DT	denotes	a
T7639	30874-30878	NN	denotes	Sox9
T7638	30879-30884	NN	denotes	probe
T7640	30884-30885	.	denotes	.
T7641	30885-31091	sentence	denotes	Similar analysis at E10.5 and E11.5 demonstrated a relatively normal process of condensation of the proximal skeletal elements (Figure S1H–S1M), despite the absence of detectable Bmp2 and Bmp4 transcripts.
T7642	30886-30893	JJ	denotes	Similar
T7643	30894-30902	NN	denotes	analysis
T7645	30903-30905	IN	denotes	at
T7646	30906-30911	NN	denotes	E10.5
T7647	30912-30915	CC	denotes	and
T7648	30916-30921	NN	denotes	E11.5
T7644	30922-30934	VBD	denotes	demonstrated
T7649	30935-30936	DT	denotes	a
T7651	30937-30947	RB	denotes	relatively
T7652	30948-30954	JJ	denotes	normal
T7650	30955-30962	NN	denotes	process
T7653	30963-30965	IN	denotes	of
T7654	30966-30978	NN	denotes	condensation
T7655	30979-30981	IN	denotes	of
T7656	30982-30985	DT	denotes	the
T7658	30986-30994	JJ	denotes	proximal
T7659	30995-31003	JJ	denotes	skeletal
T7657	31004-31012	NNS	denotes	elements
T7660	31013-31014	-LRB-	denotes	(
T7662	31014-31020	NN	denotes	Figure
T7661	31021-31024	NN	denotes	S1H
T7663	31024-31025	SYM	denotes	–
T7664	31025-31028	NN	denotes	S1M
T7665	31028-31029	-RRB-	denotes	)
T7666	31029-31031	,	denotes	,
T7667	31031-31038	IN	denotes	despite
T7668	31039-31042	DT	denotes	the
T7669	31043-31050	NN	denotes	absence
T7670	31051-31053	IN	denotes	of
T7671	31054-31064	JJ	denotes	detectable
T7673	31065-31069	NN	denotes	Bmp2
T7674	31070-31073	CC	denotes	and
T7675	31074-31078	NN	denotes	Bmp4
T7672	31079-31090	NNS	denotes	transcripts
T7676	31090-31091	.	denotes	.
T7677	31091-31294	sentence	denotes	As shown in Figure S1N and S1O, expression of Col II, a chondrocyte marker, is observed in the BMP2, BMP4–deficient cartilage (Figure S1O) as early as E12.5, similar to a wild-type control (Figure S1N).
T7678	31092-31094	IN	denotes	As
T7679	31095-31100	VBN	denotes	shown
T7681	31101-31103	IN	denotes	in
T7682	31104-31110	NN	denotes	Figure
T7683	31111-31114	NN	denotes	S1N
T7684	31115-31118	CC	denotes	and
T7685	31119-31122	NN	denotes	S1O
T7686	31122-31124	,	denotes	,
T7687	31124-31134	NN	denotes	expression
T7688	31135-31137	IN	denotes	of
T7689	31138-31141	NN	denotes	Col
T7690	31142-31144	CD	denotes	II
T7691	31144-31146	,	denotes	,
T7692	31146-31147	DT	denotes	a
T7694	31148-31159	NN	denotes	chondrocyte
T7693	31160-31166	NN	denotes	marker
T7695	31166-31168	,	denotes	,
T7696	31168-31170	VBZ	denotes	is
T7680	31171-31179	VBN	denotes	observed
T7697	31180-31182	IN	denotes	in
T7698	31183-31186	DT	denotes	the
T7700	31187-31191	NN	denotes	BMP2
T7702	31191-31193	,	denotes	,
T7701	31193-31197	NN	denotes	BMP4
T7704	31197-31198	HYPH	denotes	–
T7703	31198-31207	JJ	denotes	deficient
T7699	31208-31217	NN	denotes	cartilage
T7705	31218-31219	-LRB-	denotes	(
T7707	31219-31225	NN	denotes	Figure
T7706	31226-31229	NN	denotes	S1O
T7708	31229-31230	-RRB-	denotes	)
T7709	31231-31233	RB	denotes	as
T7710	31234-31239	RB	denotes	early
T7711	31240-31242	IN	denotes	as
T7712	31243-31248	NN	denotes	E12.5
T7713	31248-31250	,	denotes	,
T7714	31250-31257	JJ	denotes	similar
T7715	31258-31260	IN	denotes	to
T7716	31261-31262	DT	denotes	a
T7718	31263-31267	JJ	denotes	wild
T7720	31267-31268	HYPH	denotes	-
T7719	31268-31272	NN	denotes	type
T7717	31273-31280	NN	denotes	control
T7721	31281-31282	-LRB-	denotes	(
T7723	31282-31288	NN	denotes	Figure
T7722	31289-31292	NN	denotes	S1N
T7724	31292-31293	-RRB-	denotes	)
T7725	31293-31294	.	denotes	.
T7726	31294-31521	sentence	denotes	Similarly, at E13.5, the entire limb skeleton could be visualized undergoing chondrogenesis by Alcian blue staining, albeit in a defective pattern, in the BMP2, BMP4–deficient limb buds (Figure 4A and 4B and unpublished data).
T7727	31295-31304	RB	denotes	Similarly
T7729	31304-31306	,	denotes	,
T7730	31306-31308	IN	denotes	at
T7731	31309-31314	NN	denotes	E13.5
T7732	31314-31316	,	denotes	,
T7733	31316-31319	DT	denotes	the
T7735	31320-31326	JJ	denotes	entire
T7736	31327-31331	NN	denotes	limb
T7734	31332-31340	NN	denotes	skeleton
T7737	31341-31346	MD	denotes	could
T7738	31347-31349	VB	denotes	be
T7728	31350-31360	VBN	denotes	visualized
T7739	31361-31371	VBG	denotes	undergoing
T7740	31372-31386	NN	denotes	chondrogenesis
T7741	31387-31389	IN	denotes	by
T7742	31390-31396	JJ	denotes	Alcian
T7744	31397-31401	JJ	denotes	blue
T7743	31402-31410	NN	denotes	staining
T7745	31410-31412	,	denotes	,
T7746	31412-31418	IN	denotes	albeit
T7747	31419-31421	IN	denotes	in
T7748	31422-31423	DT	denotes	a
T7750	31424-31433	JJ	denotes	defective
T7749	31434-31441	NN	denotes	pattern
T7751	31441-31443	,	denotes	,
T7752	31443-31445	IN	denotes	in
T7753	31446-31449	DT	denotes	the
T7755	31450-31454	NN	denotes	BMP2
T7757	31454-31456	,	denotes	,
T7756	31456-31460	NN	denotes	BMP4
T7759	31460-31461	HYPH	denotes	–
T7758	31461-31470	JJ	denotes	deficient
T7760	31471-31475	NN	denotes	limb
T7754	31476-31480	NNS	denotes	buds
T7761	31481-31482	-LRB-	denotes	(
T7763	31482-31488	NN	denotes	Figure
T7762	31489-31491	NN	denotes	4A
T7764	31492-31495	CC	denotes	and
T7765	31496-31498	NN	denotes	4B
T7766	31499-31502	CC	denotes	and
T7767	31503-31514	JJ	denotes	unpublished
T7768	31515-31519	NNS	denotes	data
T7769	31519-31520	-RRB-	denotes	)
T7770	31520-31521	.	denotes	.
T7771	31521-32569	sentence	denotes	Figure 4 Chondrogenesis Starts and Proceeds Normally Even in the Absence of BMP2 and BMP4 (A and B) Whole mount skeletons from E13.5 embryos that are stained with alcian blue. (A) Wild-type embryo, (B) Bmp2C/C; Bmp4C/C; Prx1::cre embryo. Black arrow in (B) shows the fusion of the zeugopod and stylopod. (C and D) Hematoxylin and eosin–stained sagittal sections of humeri from E13.5 wild-type (C) and Bmp2C/C; Bmp4C/C; Prx1::cre (D) embryos. Thick red arrow in (C) shows the hypertrophic region. (E and F) Sagittal sections of wild-type (E) and Bmp2C/C; Bmp4C/C; Prx1::cre (F) humeri from E15.5 embryos were hybridized with digoxigenin-labeled antisense rioprobes for ColX. (G and H) Sagittal sections of forelimbs from E13.5 wild-type and BMP2, BMP4–deficient embryos, respectively, stained with radioactive riboprobes for Bmp7 mRNA. (G′) and (H′) show the bright field views of (G) and (H), respectively. As the cartilage elements grow, a wave of hypertrophic differentiation occurs from the center toward the distal ends (reviewed in [39–41]).
T7772	32430-32432	IN	denotes	As
T7774	32433-32436	DT	denotes	the
T7776	32437-32446	NN	denotes	cartilage
T7775	32447-32455	NNS	denotes	elements
T7773	32456-32460	VBP	denotes	grow
T7778	32460-32462	,	denotes	,
T7779	32462-32463	DT	denotes	a
T7780	32464-32468	NN	denotes	wave
T7781	32469-32471	IN	denotes	of
T7782	32472-32484	JJ	denotes	hypertrophic
T7783	32485-32500	NN	denotes	differentiation
T7777	32501-32507	VBZ	denotes	occurs
T7784	32508-32512	IN	denotes	from
T7785	32513-32516	DT	denotes	the
T7786	32517-32523	NN	denotes	center
T7787	32524-32530	IN	denotes	toward
T7788	32531-32534	DT	denotes	the
T7790	32535-32541	JJ	denotes	distal
T7789	32542-32546	NNS	denotes	ends
T7791	32547-32548	-LRB-	denotes	(
T7792	32548-32556	VBN	denotes	reviewed
T7793	32557-32559	IN	denotes	in
T7794	32560-32561	-LRB-	denotes	[
T7795	32561-32563	CD	denotes	39
T7796	32563-32564	SYM	denotes	–
T7797	32564-32566	CD	denotes	41
T7798	32566-32567	-RRB-	denotes	]
T7799	32567-32568	-RRB-	denotes	)
T7800	32568-32569	.	denotes	.
T7801	32569-32778	sentence	denotes	Histological examination of the humeri at E13.5 showed that while chondrogenic differentiation had taken place in the BMP2, BMP4–deficient humerus, hypertrophic differentiation was delayed (Figure 4C and 4D).
T7802	32570-32582	JJ	denotes	Histological
T7803	32583-32594	NN	denotes	examination
T7805	32595-32597	IN	denotes	of
T7806	32598-32601	DT	denotes	the
T7807	32602-32608	NNS	denotes	humeri
T7808	32609-32611	IN	denotes	at
T7809	32612-32617	NN	denotes	E13.5
T7804	32618-32624	VBD	denotes	showed
T7810	32625-32629	IN	denotes	that
T7812	32630-32635	IN	denotes	while
T7814	32636-32648	JJ	denotes	chondrogenic
T7815	32649-32664	NN	denotes	differentiation
T7816	32665-32668	VBD	denotes	had
T7813	32669-32674	VBN	denotes	taken
T7817	32675-32680	NN	denotes	place
T7818	32681-32683	IN	denotes	in
T7819	32684-32687	DT	denotes	the
T7821	32688-32692	NN	denotes	BMP2
T7823	32692-32694	,	denotes	,
T7822	32694-32698	NN	denotes	BMP4
T7825	32698-32699	HYPH	denotes	–
T7824	32699-32708	JJ	denotes	deficient
T7820	32709-32716	NN	denotes	humerus
T7826	32716-32718	,	denotes	,
T7827	32718-32730	JJ	denotes	hypertrophic
T7828	32731-32746	NN	denotes	differentiation
T7829	32747-32750	VBD	denotes	was
T7811	32751-32758	VBN	denotes	delayed
T7830	32759-32760	-LRB-	denotes	(
T7832	32760-32766	NN	denotes	Figure
T7831	32767-32769	NN	denotes	4C
T7833	32770-32773	CC	denotes	and
T7834	32774-32776	NN	denotes	4D
T7835	32776-32777	-RRB-	denotes	)
T7836	32777-32778	.	denotes	.
T7837	32778-32897	sentence	denotes	However, by E15.5, hypertrophy was evident in these elements as seen by type X collagen expression (Figure 4E and 4F).
T7838	32779-32786	RB	denotes	However
T7840	32786-32788	,	denotes	,
T7841	32788-32790	IN	denotes	by
T7842	32791-32796	NN	denotes	E15.5
T7843	32796-32798	,	denotes	,
T7844	32798-32809	NN	denotes	hypertrophy
T7839	32810-32813	VBD	denotes	was
T7845	32814-32821	JJ	denotes	evident
T7846	32822-32824	IN	denotes	in
T7847	32825-32830	DT	denotes	these
T7848	32831-32839	NNS	denotes	elements
T7849	32840-32842	IN	denotes	as
T7850	32843-32847	VBN	denotes	seen
T7851	32848-32850	IN	denotes	by
T7852	32851-32855	NN	denotes	type
T7854	32856-32857	SYM	denotes	X
T7855	32858-32866	NN	denotes	collagen
T7853	32867-32877	NN	denotes	expression
T7856	32878-32879	-LRB-	denotes	(
T7858	32879-32885	NN	denotes	Figure
T7857	32886-32888	NN	denotes	4E
T7859	32889-32892	CC	denotes	and
T7860	32893-32895	NN	denotes	4F
T7861	32895-32896	-RRB-	denotes	)
T7862	32896-32897	.	denotes	.
T7863	32897-33034	sentence	denotes	The hypertrophic cells in BMP2, BMP4–deficient limb also express late hypertrophic markers, such as osteopontin [42] (unpublished data).
T7864	32898-32901	DT	denotes	The
T7866	32902-32914	JJ	denotes	hypertrophic
T7865	32915-32920	NNS	denotes	cells
T7868	32921-32923	IN	denotes	in
T7869	32924-32928	NN	denotes	BMP2
T7871	32928-32930	,	denotes	,
T7870	32930-32934	NN	denotes	BMP4
T7873	32934-32935	HYPH	denotes	–
T7872	32935-32944	JJ	denotes	deficient
T7874	32945-32949	NN	denotes	limb
T7875	32950-32954	RB	denotes	also
T7867	32955-32962	VBP	denotes	express
T7876	32963-32967	JJ	denotes	late
T7878	32968-32980	JJ	denotes	hypertrophic
T7877	32981-32988	NNS	denotes	markers
T7879	32988-32990	,	denotes	,
T7880	32990-32994	JJ	denotes	such
T7881	32995-32997	IN	denotes	as
T7882	32998-33009	NN	denotes	osteopontin
T7883	33010-33011	-LRB-	denotes	[
T7884	33011-33013	CD	denotes	42
T7885	33013-33014	-RRB-	denotes	]
T7886	33015-33016	-LRB-	denotes	(
T7888	33016-33027	JJ	denotes	unpublished
T7887	33028-33032	NNS	denotes	data
T7889	33032-33033	-RRB-	denotes	)
T7890	33033-33034	.	denotes	.
T7891	33034-33267	sentence	denotes	To examine whether the successful execution of chondrogenesis in the BMP2, BMP4–deficient limbs is a result of compensatory upregulation of other ostegenic BMPs, we carried out in situ hybridization for Bmp6 and Bmp7 in these limbs.
T7892	33035-33037	TO	denotes	To
T7893	33038-33045	VB	denotes	examine
T7895	33046-33053	IN	denotes	whether
T7897	33054-33057	DT	denotes	the
T7899	33058-33068	JJ	denotes	successful
T7898	33069-33078	NN	denotes	execution
T7900	33079-33081	IN	denotes	of
T7901	33082-33096	NN	denotes	chondrogenesis
T7902	33097-33099	IN	denotes	in
T7903	33100-33103	DT	denotes	the
T7905	33104-33108	NN	denotes	BMP2
T7907	33108-33110	,	denotes	,
T7906	33110-33114	NN	denotes	BMP4
T7909	33114-33115	HYPH	denotes	–
T7908	33115-33124	JJ	denotes	deficient
T7904	33125-33130	NNS	denotes	limbs
T7896	33131-33133	VBZ	denotes	is
T7910	33134-33135	DT	denotes	a
T7911	33136-33142	NN	denotes	result
T7912	33143-33145	IN	denotes	of
T7913	33146-33158	JJ	denotes	compensatory
T7914	33159-33171	NN	denotes	upregulation
T7915	33172-33174	IN	denotes	of
T7916	33175-33180	JJ	denotes	other
T7918	33181-33190	JJ	denotes	ostegenic
T7917	33191-33195	NNS	denotes	BMPs
T7919	33195-33197	,	denotes	,
T7920	33197-33199	PRP	denotes	we
T7894	33200-33207	VBD	denotes	carried
T7921	33208-33211	RP	denotes	out
T7922	33212-33214	FW	denotes	in
T7923	33215-33219	FW	denotes	situ
T7924	33220-33233	NN	denotes	hybridization
T7925	33234-33237	IN	denotes	for
T7926	33238-33242	NN	denotes	Bmp6
T7927	33243-33246	CC	denotes	and
T7928	33247-33251	NN	denotes	Bmp7
T7929	33252-33254	IN	denotes	in
T7930	33255-33260	DT	denotes	these
T7931	33261-33266	NNS	denotes	limbs
T7932	33266-33267	.	denotes	.
T7933	33267-33451	sentence	denotes	At E13.5, we observed no increase in expression of Bmp6 (Figure S1P–S1S) or Bmp7 (compare Figure 4H with Figure 4G) mRNA in the mutant tissues compared to their wild-type counterparts
T7934	33268-33270	IN	denotes	At
T7936	33271-33276	NN	denotes	E13.5
T7937	33276-33278	,	denotes	,
T7938	33278-33280	PRP	denotes	we
T7935	33281-33289	VBD	denotes	observed
T7939	33290-33292	DT	denotes	no
T7940	33293-33301	NN	denotes	increase
T7941	33302-33304	IN	denotes	in
T7942	33305-33315	NN	denotes	expression
T7943	33316-33318	IN	denotes	of
T7944	33319-33323	NN	denotes	Bmp6
T7946	33324-33325	-LRB-	denotes	(
T7948	33325-33331	NN	denotes	Figure
T7947	33332-33335	NN	denotes	S1P
T7949	33335-33336	SYM	denotes	–
T7950	33336-33339	NN	denotes	S1S
T7951	33339-33340	-RRB-	denotes	)
T7952	33341-33343	CC	denotes	or
T7953	33344-33348	NN	denotes	Bmp7
T7954	33349-33350	-LRB-	denotes	(
T7955	33350-33357	VB	denotes	compare
T7956	33358-33364	NN	denotes	Figure
T7957	33365-33367	NN	denotes	4H
T7958	33368-33372	IN	denotes	with
T7959	33373-33379	NN	denotes	Figure
T7960	33380-33382	NN	denotes	4G
T7961	33382-33383	-RRB-	denotes	)
T7945	33384-33388	NN	denotes	mRNA
T7962	33389-33391	IN	denotes	in
T7963	33392-33395	DT	denotes	the
T7965	33396-33402	NN	denotes	mutant
T7964	33403-33410	NNS	denotes	tissues
T7966	33411-33419	VBN	denotes	compared
T7967	33420-33422	IN	denotes	to
T7968	33423-33428	PRP$	denotes	their
T7970	33429-33433	JJ	denotes	wild
T7972	33433-33434	HYPH	denotes	-
T7971	33434-33438	NN	denotes	type
T7969	33439-33451	NNS	denotes	counterparts
T8396	33453-33465	NN	denotes	Osteogenesis
T8398	33466-33468	VBZ	denotes	Is
T8397	33469-33478	VBN	denotes	Initiated
T8399	33479-33487	RB	denotes	Normally
T8400	33488-33490	IN	denotes	in
T8401	33491-33495	NN	denotes	BMP2
T8403	33495-33497	,	denotes	,
T8402	33497-33501	NN	denotes	BMP4
T8405	33501-33502	HYPH	denotes	–
T8404	33502-33511	JJ	denotes	Deficient
T8407	33512-33516	NN	denotes	Limb
T8406	33517-33521	NNS	denotes	Buds
T8408	33521-33684	sentence	denotes	Bone formation first occurs at the interface between the late hypertrophic chondrocytes and the surrounding perichondrium, a region identified as the bone collar.
T8409	33522-33526	NN	denotes	Bone
T8410	33527-33536	NN	denotes	formation
T8412	33537-33542	RB	denotes	first
T8411	33543-33549	VBZ	denotes	occurs
T8413	33550-33552	IN	denotes	at
T8414	33553-33556	DT	denotes	the
T8415	33557-33566	NN	denotes	interface
T8416	33567-33574	IN	denotes	between
T8417	33575-33578	DT	denotes	the
T8419	33579-33583	JJ	denotes	late
T8420	33584-33596	JJ	denotes	hypertrophic
T8418	33597-33609	NNS	denotes	chondrocytes
T8421	33610-33613	CC	denotes	and
T8422	33614-33617	DT	denotes	the
T8424	33618-33629	VBG	denotes	surrounding
T8423	33630-33643	NN	denotes	perichondrium
T8425	33643-33645	,	denotes	,
T8426	33645-33646	DT	denotes	a
T8427	33647-33653	NN	denotes	region
T8428	33654-33664	VBN	denotes	identified
T8429	33665-33667	IN	denotes	as
T8430	33668-33671	DT	denotes	the
T8432	33672-33676	NN	denotes	bone
T8431	33677-33683	NN	denotes	collar
T8433	33683-33684	.	denotes	.
T8434	33684-33858	sentence	denotes	We examined whether the osteogenesis program is initiated in the BMP2, BMP4–deficient limbs using several histological and molecular criteria, as described below (Figure 5).
T8435	33685-33687	PRP	denotes	We
T8436	33688-33696	VBD	denotes	examined
T8437	33697-33704	IN	denotes	whether
T8439	33705-33708	DT	denotes	the
T8441	33709-33721	NN	denotes	osteogenesis
T8440	33722-33729	NN	denotes	program
T8442	33730-33732	VBZ	denotes	is
T8438	33733-33742	VBN	denotes	initiated
T8443	33743-33745	IN	denotes	in
T8444	33746-33749	DT	denotes	the
T8446	33750-33754	NN	denotes	BMP2
T8448	33754-33756	,	denotes	,
T8447	33756-33760	NN	denotes	BMP4
T8450	33760-33761	HYPH	denotes	–
T8449	33761-33770	JJ	denotes	deficient
T8445	33771-33776	NNS	denotes	limbs
T8451	33777-33782	VBG	denotes	using
T8452	33783-33790	JJ	denotes	several
T8454	33791-33803	JJ	denotes	histological
T8455	33804-33807	CC	denotes	and
T8456	33808-33817	JJ	denotes	molecular
T8453	33818-33826	NNS	denotes	criteria
T8457	33826-33828	,	denotes	,
T8458	33828-33830	IN	denotes	as
T8459	33831-33840	VBN	denotes	described
T8460	33841-33846	RB	denotes	below
T8461	33847-33848	-LRB-	denotes	(
T8462	33848-33854	NN	denotes	Figure
T8463	33855-33856	CD	denotes	5
T8464	33856-33857	-RRB-	denotes	)
T8465	33857-33858	.	denotes	.
T8466	33858-33992	sentence	denotes	Adjacent to the hypertrophic cells, a bone collar is laid down normally in the absence of BMP2 and BMP4 (Figure 5A and 5B, red star).
T8467	33859-33867	JJ	denotes	Adjacent
T8469	33868-33870	IN	denotes	to
T8470	33871-33874	DT	denotes	the
T8472	33875-33887	JJ	denotes	hypertrophic
T8471	33888-33893	NNS	denotes	cells
T8473	33893-33895	,	denotes	,
T8474	33895-33896	DT	denotes	a
T8476	33897-33901	NN	denotes	bone
T8475	33902-33908	NN	denotes	collar
T8477	33909-33911	VBZ	denotes	is
T8468	33912-33916	VBN	denotes	laid
T8478	33917-33921	RP	denotes	down
T8479	33922-33930	RB	denotes	normally
T8480	33931-33933	IN	denotes	in
T8481	33934-33937	DT	denotes	the
T8482	33938-33945	NN	denotes	absence
T8483	33946-33948	IN	denotes	of
T8484	33949-33953	NN	denotes	BMP2
T8485	33954-33957	CC	denotes	and
T8486	33958-33962	NN	denotes	BMP4
T8487	33963-33964	-LRB-	denotes	(
T8489	33964-33970	NN	denotes	Figure
T8490	33971-33973	NN	denotes	5A
T8491	33974-33977	CC	denotes	and
T8492	33978-33980	NN	denotes	5B
T8493	33980-33982	,	denotes	,
T8494	33982-33985	JJ	denotes	red
T8488	33986-33990	NN	denotes	star
T8495	33990-33991	-RRB-	denotes	)
T8496	33991-33992	.	denotes	.
T8497	33992-34165	sentence	denotes	As can be seen in sagittal sections through an E15.5 humerus, the cells embedded in the mineralized matrix express the osteoblast marker Col I as normal (Figure 5C and 5D).
T8498	33993-33995	IN	denotes	As
T8500	33996-33999	MD	denotes	can
T8501	34000-34002	VB	denotes	be
T8499	34003-34007	VBN	denotes	seen
T8503	34008-34010	IN	denotes	in
T8504	34011-34019	JJ	denotes	sagittal
T8505	34020-34028	NNS	denotes	sections
T8506	34029-34036	IN	denotes	through
T8507	34037-34039	DT	denotes	an
T8509	34040-34045	NN	denotes	E15.5
T8508	34046-34053	NN	denotes	humerus
T8510	34053-34055	,	denotes	,
T8511	34055-34058	DT	denotes	the
T8512	34059-34064	NNS	denotes	cells
T8513	34065-34073	VBN	denotes	embedded
T8514	34074-34076	IN	denotes	in
T8515	34077-34080	DT	denotes	the
T8517	34081-34092	VBN	denotes	mineralized
T8516	34093-34099	NN	denotes	matrix
T8502	34100-34107	VBP	denotes	express
T8518	34108-34111	DT	denotes	the
T8520	34112-34122	NN	denotes	osteoblast
T8519	34123-34129	NN	denotes	marker
T8521	34130-34133	NN	denotes	Col
T8522	34134-34135	CD	denotes	I
T8523	34136-34138	IN	denotes	as
T8524	34139-34145	JJ	denotes	normal
T8525	34146-34147	-LRB-	denotes	(
T8527	34147-34153	NN	denotes	Figure
T8526	34154-34156	NN	denotes	5C
T8528	34157-34160	CC	denotes	and
T8529	34161-34163	NN	denotes	5D
T8530	34163-34164	-RRB-	denotes	)
T8531	34164-34165	.	denotes	.
T8532	34165-34453	sentence	denotes	By E17.5, the mineralized cartilage that makes up the late hypertrophic zone of the growth plate normally undergoes removal by osteoclasts (Figure 5G, brown-stained cells, red arrows), resulting in the formation of a bone marrow cavity (red arrow in Figure 5E and 5I, see also Figure 6).
T8533	34166-34168	IN	denotes	By
T8535	34169-34174	NN	denotes	E17.5
T8536	34174-34176	,	denotes	,
T8537	34176-34179	DT	denotes	the
T8539	34180-34191	JJ	denotes	mineralized
T8538	34192-34201	NN	denotes	cartilage
T8540	34202-34206	WDT	denotes	that
T8541	34207-34212	VBZ	denotes	makes
T8542	34213-34215	RP	denotes	up
T8543	34216-34219	DT	denotes	the
T8545	34220-34224	JJ	denotes	late
T8546	34225-34237	JJ	denotes	hypertrophic
T8544	34238-34242	NN	denotes	zone
T8547	34243-34245	IN	denotes	of
T8548	34246-34249	DT	denotes	the
T8550	34250-34256	NN	denotes	growth
T8549	34257-34262	NN	denotes	plate
T8551	34263-34271	RB	denotes	normally
T8534	34272-34281	VBZ	denotes	undergoes
T8552	34282-34289	NN	denotes	removal
T8553	34290-34292	IN	denotes	by
T8554	34293-34304	NNS	denotes	osteoclasts
T8555	34305-34306	-LRB-	denotes	(
T8557	34306-34312	NN	denotes	Figure
T8558	34313-34315	NN	denotes	5G
T8559	34315-34317	,	denotes	,
T8560	34317-34322	JJ	denotes	brown
T8562	34322-34323	HYPH	denotes	-
T8561	34323-34330	VBN	denotes	stained
T8563	34331-34336	NNS	denotes	cells
T8564	34336-34338	,	denotes	,
T8565	34338-34341	JJ	denotes	red
T8556	34342-34348	NNS	denotes	arrows
T8566	34348-34349	-RRB-	denotes	)
T8567	34349-34351	,	denotes	,
T8568	34351-34360	VBG	denotes	resulting
T8569	34361-34363	IN	denotes	in
T8570	34364-34367	DT	denotes	the
T8571	34368-34377	NN	denotes	formation
T8572	34378-34380	IN	denotes	of
T8573	34381-34382	DT	denotes	a
T8575	34383-34387	NN	denotes	bone
T8576	34388-34394	NN	denotes	marrow
T8574	34395-34401	NN	denotes	cavity
T8577	34402-34403	-LRB-	denotes	(
T8579	34403-34406	JJ	denotes	red
T8578	34407-34412	NN	denotes	arrow
T8580	34413-34415	IN	denotes	in
T8581	34416-34422	NN	denotes	Figure
T8582	34423-34425	NN	denotes	5E
T8583	34426-34429	CC	denotes	and
T8584	34430-34432	NN	denotes	5I
T8585	34432-34434	,	denotes	,
T8586	34434-34437	VB	denotes	see
T8587	34438-34442	RB	denotes	also
T8588	34443-34449	NN	denotes	Figure
T8589	34450-34451	CD	denotes	6
T8590	34451-34452	-RRB-	denotes	)
T8591	34452-34453	.	denotes	.
T8592	34453-34670	sentence	denotes	Osteoprogenitor cells from bone collar region now populate this site and deposit osteoid, forming the trabecular bone in the bone marrow cavity (Figure 6E, red arrow) and cortical bone at birth (Figure 5I, asterisk).
T8593	34454-34469	NN	denotes	Osteoprogenitor
T8594	34470-34475	NNS	denotes	cells
T8596	34476-34480	IN	denotes	from
T8597	34481-34485	NN	denotes	bone
T8599	34486-34492	NN	denotes	collar
T8598	34493-34499	NN	denotes	region
T8600	34500-34503	RB	denotes	now
T8595	34504-34512	VBP	denotes	populate
T8601	34513-34517	DT	denotes	this
T8602	34518-34522	NN	denotes	site
T8603	34523-34526	CC	denotes	and
T8604	34527-34534	VBP	denotes	deposit
T8605	34535-34542	NN	denotes	osteoid
T8606	34542-34544	,	denotes	,
T8607	34544-34551	VBG	denotes	forming
T8608	34552-34555	DT	denotes	the
T8610	34556-34566	JJ	denotes	trabecular
T8609	34567-34571	NN	denotes	bone
T8611	34572-34574	IN	denotes	in
T8612	34575-34578	DT	denotes	the
T8614	34579-34583	NN	denotes	bone
T8615	34584-34590	NN	denotes	marrow
T8613	34591-34597	NN	denotes	cavity
T8616	34598-34599	-LRB-	denotes	(
T8618	34599-34605	NN	denotes	Figure
T8619	34606-34608	NN	denotes	6E
T8620	34608-34610	,	denotes	,
T8621	34610-34613	JJ	denotes	red
T8617	34614-34619	NN	denotes	arrow
T8622	34619-34620	-RRB-	denotes	)
T8623	34621-34624	CC	denotes	and
T8624	34625-34633	JJ	denotes	cortical
T8625	34634-34638	NN	denotes	bone
T8626	34639-34641	IN	denotes	at
T8627	34642-34647	NN	denotes	birth
T8628	34648-34649	-LRB-	denotes	(
T8630	34649-34655	NN	denotes	Figure
T8631	34656-34658	NN	denotes	5I
T8632	34658-34660	,	denotes	,
T8629	34660-34668	NN	denotes	asterisk
T8633	34668-34669	-RRB-	denotes	)
T8634	34669-34670	.	denotes	.
T8635	34670-34800	sentence	denotes	In contrast, in E17.5 limbs deficient in BMP2 and BMP4, there is an overall delay in the normal endochondral process (Figure 5F).
T8636	34671-34673	IN	denotes	In
T8638	34674-34682	NN	denotes	contrast
T8639	34682-34684	,	denotes	,
T8640	34684-34686	IN	denotes	in
T8641	34687-34692	NN	denotes	E17.5
T8642	34693-34698	NNS	denotes	limbs
T8643	34699-34708	JJ	denotes	deficient
T8644	34709-34711	IN	denotes	in
T8645	34712-34716	NN	denotes	BMP2
T8646	34717-34720	CC	denotes	and
T8647	34721-34725	NN	denotes	BMP4
T8648	34725-34727	,	denotes	,
T8649	34727-34732	EX	denotes	there
T8637	34733-34735	VBZ	denotes	is
T8650	34736-34738	DT	denotes	an
T8652	34739-34746	JJ	denotes	overall
T8651	34747-34752	NN	denotes	delay
T8653	34753-34755	IN	denotes	in
T8654	34756-34759	DT	denotes	the
T8656	34760-34766	JJ	denotes	normal
T8657	34767-34779	JJ	denotes	endochondral
T8655	34780-34787	NN	denotes	process
T8658	34788-34789	-LRB-	denotes	(
T8660	34789-34795	NN	denotes	Figure
T8659	34796-34798	NN	denotes	5F
T8661	34798-34799	-RRB-	denotes	)
T8662	34799-34800	.	denotes	.
T8663	34800-35063	sentence	denotes	While vascularization occurs in Bmp2C/C; Bmp4C/C; Prx1::cre forelimbs, few osteoclasts are recruited to the mineralized cartilage (compare Figure 5G and Figure 5H, red arrows), resulting in a delay in bone marrow cavity formation in the absence of BMP2 and BMP4.
T8664	34801-34806	IN	denotes	While
T8666	34807-34822	NN	denotes	vascularization
T8665	34823-34829	VBZ	denotes	occurs
T8668	34830-34832	IN	denotes	in
T8669	34833-34838	NN	denotes	Bmp2C
T8671	34838-34839	HYPH	denotes	/
T8670	34839-34840	NN	denotes	C
T8673	34840-34841	:	denotes	;
T8674	34842-34847	NN	denotes	Bmp4C
T8676	34847-34848	HYPH	denotes	/
T8675	34848-34849	NN	denotes	C
T8677	34849-34850	:	denotes	;
T8678	34851-34855	NN	denotes	Prx1
T8680	34855-34856	HYPH	denotes	:
T8681	34856-34857	HYPH	denotes	:
T8679	34857-34860	NN	denotes	cre
T8672	34861-34870	NNS	denotes	forelimbs
T8682	34870-34872	,	denotes	,
T8683	34872-34875	JJ	denotes	few
T8684	34876-34887	NNS	denotes	osteoclasts
T8685	34888-34891	VBP	denotes	are
T8667	34892-34901	VBN	denotes	recruited
T8686	34902-34904	IN	denotes	to
T8687	34905-34908	DT	denotes	the
T8689	34909-34920	VBN	denotes	mineralized
T8688	34921-34930	NN	denotes	cartilage
T8690	34931-34932	-LRB-	denotes	(
T8692	34932-34939	VB	denotes	compare
T8693	34940-34946	NN	denotes	Figure
T8694	34947-34949	NN	denotes	5G
T8695	34950-34953	CC	denotes	and
T8696	34954-34960	NN	denotes	Figure
T8697	34961-34963	NN	denotes	5H
T8698	34963-34965	,	denotes	,
T8699	34965-34968	JJ	denotes	red
T8691	34969-34975	NNS	denotes	arrows
T8700	34975-34976	-RRB-	denotes	)
T8701	34976-34978	,	denotes	,
T8702	34978-34987	VBG	denotes	resulting
T8703	34988-34990	IN	denotes	in
T8704	34991-34992	DT	denotes	a
T8705	34993-34998	NN	denotes	delay
T8706	34999-35001	IN	denotes	in
T8707	35002-35006	NN	denotes	bone
T8708	35007-35013	NN	denotes	marrow
T8710	35014-35020	NN	denotes	cavity
T8709	35021-35030	NN	denotes	formation
T8711	35031-35033	IN	denotes	in
T8712	35034-35037	DT	denotes	the
T8713	35038-35045	NN	denotes	absence
T8714	35046-35048	IN	denotes	of
T8715	35049-35053	NN	denotes	BMP2
T8716	35054-35057	CC	denotes	and
T8717	35058-35062	NN	denotes	BMP4
T8718	35062-35063	.	denotes	.
T8719	35063-35321	sentence	denotes	In fact, bone marrow formation and trabecular bone formation have not yet started at birth in the absence of BMP2 and BMP4 (Figure 5J), and the morphology of Bmp2C/C; Bmp4C/C; Prx1::cre bones at birth is most similar to control embryos at E17.5 (Figure 5E).
T8720	35064-35066	IN	denotes	In
T8722	35067-35071	NN	denotes	fact
T8723	35071-35073	,	denotes	,
T8724	35073-35077	NN	denotes	bone
T8725	35078-35084	NN	denotes	marrow
T8726	35085-35094	NN	denotes	formation
T8727	35095-35098	CC	denotes	and
T8728	35099-35109	JJ	denotes	trabecular
T8730	35110-35114	NN	denotes	bone
T8729	35115-35124	NN	denotes	formation
T8731	35125-35129	VBP	denotes	have
T8732	35130-35133	RB	denotes	not
T8733	35134-35137	RB	denotes	yet
T8721	35138-35145	VBN	denotes	started
T8734	35146-35148	IN	denotes	at
T8735	35149-35154	NN	denotes	birth
T8736	35155-35157	IN	denotes	in
T8737	35158-35161	DT	denotes	the
T8738	35162-35169	NN	denotes	absence
T8739	35170-35172	IN	denotes	of
T8740	35173-35177	NN	denotes	BMP2
T8741	35178-35181	CC	denotes	and
T8742	35182-35186	NN	denotes	BMP4
T8743	35187-35188	-LRB-	denotes	(
T8745	35188-35194	NN	denotes	Figure
T8744	35195-35197	NN	denotes	5J
T8746	35197-35198	-RRB-	denotes	)
T8747	35198-35200	,	denotes	,
T8748	35200-35203	CC	denotes	and
T8749	35204-35207	DT	denotes	the
T8750	35208-35218	NN	denotes	morphology
T8752	35219-35221	IN	denotes	of
T8753	35222-35227	NN	denotes	Bmp2C
T8755	35227-35228	HYPH	denotes	/
T8754	35228-35229	NN	denotes	C
T8757	35229-35230	:	denotes	;
T8758	35231-35236	NN	denotes	Bmp4C
T8760	35236-35237	HYPH	denotes	/
T8759	35237-35238	NN	denotes	C
T8761	35238-35239	:	denotes	;
T8762	35240-35244	NN	denotes	Prx1
T8764	35244-35245	HYPH	denotes	:
T8765	35245-35246	HYPH	denotes	:
T8763	35246-35249	NN	denotes	cre
T8756	35250-35255	NNS	denotes	bones
T8766	35256-35258	IN	denotes	at
T8767	35259-35264	NN	denotes	birth
T8751	35265-35267	VBZ	denotes	is
T8768	35268-35272	RBS	denotes	most
T8769	35273-35280	JJ	denotes	similar
T8770	35281-35283	IN	denotes	to
T8771	35284-35291	NN	denotes	control
T8772	35292-35299	NNS	denotes	embryos
T8773	35300-35302	IN	denotes	at
T8774	35303-35308	NN	denotes	E17.5
T8775	35309-35310	-LRB-	denotes	(
T8777	35310-35316	NN	denotes	Figure
T8776	35317-35319	NN	denotes	5E
T8778	35319-35320	-RRB-	denotes	)
T8779	35320-35321	.	denotes	.
T8780	35321-35553	sentence	denotes	To differentiate between a simple delay in bone formation in the absence of BMP2 and BMP4 and the inability to form bone without these BMPs, we performed histological analyses in Bmp2C/C; Bmp4C/C; Prx1::cre mice up to 3 wks of age.
T8781	35322-35324	TO	denotes	To
T8782	35325-35338	VB	denotes	differentiate
T8784	35339-35346	IN	denotes	between
T8785	35347-35348	DT	denotes	a
T8787	35349-35355	JJ	denotes	simple
T8786	35356-35361	NN	denotes	delay
T8788	35362-35364	IN	denotes	in
T8789	35365-35369	NN	denotes	bone
T8790	35370-35379	NN	denotes	formation
T8791	35380-35382	IN	denotes	in
T8792	35383-35386	DT	denotes	the
T8793	35387-35394	NN	denotes	absence
T8794	35395-35397	IN	denotes	of
T8795	35398-35402	NN	denotes	BMP2
T8796	35403-35406	CC	denotes	and
T8797	35407-35411	NN	denotes	BMP4
T8798	35412-35415	CC	denotes	and
T8799	35416-35419	DT	denotes	the
T8800	35420-35429	NN	denotes	inability
T8801	35430-35432	TO	denotes	to
T8802	35433-35437	VB	denotes	form
T8803	35438-35442	NN	denotes	bone
T8804	35443-35450	IN	denotes	without
T8805	35451-35456	DT	denotes	these
T8806	35457-35461	NNS	denotes	BMPs
T8807	35461-35463	,	denotes	,
T8808	35463-35465	PRP	denotes	we
T8783	35466-35475	VBD	denotes	performed
T8809	35476-35488	JJ	denotes	histological
T8810	35489-35497	NNS	denotes	analyses
T8811	35498-35500	IN	denotes	in
T8812	35501-35506	NN	denotes	Bmp2C
T8814	35506-35507	HYPH	denotes	/
T8813	35507-35508	NN	denotes	C
T8816	35508-35509	:	denotes	;
T8817	35510-35515	NN	denotes	Bmp4C
T8819	35515-35516	HYPH	denotes	/
T8818	35516-35517	NN	denotes	C
T8820	35517-35518	:	denotes	;
T8821	35519-35523	NN	denotes	Prx1
T8823	35523-35524	HYPH	denotes	:
T8824	35524-35525	HYPH	denotes	:
T8822	35525-35528	NN	denotes	cre
T8815	35529-35533	NNS	denotes	mice
T8825	35534-35536	RB	denotes	up
T8827	35537-35539	IN	denotes	to
T8826	35540-35541	CD	denotes	3
T8828	35542-35545	NNS	denotes	wks
T8829	35546-35548	IN	denotes	of
T8830	35549-35552	NN	denotes	age
T8831	35552-35553	.	denotes	.
T8832	35553-35714	sentence	denotes	As shown in Figure 6A through 6E, in the control mice, a bone collar is found adjacent to the zone of late hypertrophic chondrocytes at 1 wk (Figure 6A and 6B).
T8833	35554-35556	IN	denotes	As
T8834	35557-35562	VBN	denotes	shown
T8836	35563-35565	IN	denotes	in
T8837	35566-35572	NN	denotes	Figure
T8838	35573-35575	NN	denotes	6A
T8839	35576-35583	IN	denotes	through
T8840	35584-35586	NN	denotes	6E
T8841	35586-35588	,	denotes	,
T8842	35588-35590	IN	denotes	in
T8843	35591-35594	DT	denotes	the
T8845	35595-35602	NN	denotes	control
T8844	35603-35607	NNS	denotes	mice
T8846	35607-35609	,	denotes	,
T8847	35609-35610	DT	denotes	a
T8849	35611-35615	NN	denotes	bone
T8848	35616-35622	NN	denotes	collar
T8850	35623-35625	VBZ	denotes	is
T8835	35626-35631	VBN	denotes	found
T8851	35632-35640	JJ	denotes	adjacent
T8852	35641-35643	IN	denotes	to
T8853	35644-35647	DT	denotes	the
T8854	35648-35652	NN	denotes	zone
T8855	35653-35655	IN	denotes	of
T8856	35656-35660	JJ	denotes	late
T8858	35661-35673	JJ	denotes	hypertrophic
T8857	35674-35686	NNS	denotes	chondrocytes
T8859	35687-35689	IN	denotes	at
T8860	35690-35691	CD	denotes	1
T8861	35692-35694	NN	denotes	wk
T8862	35695-35696	-LRB-	denotes	(
T8864	35696-35702	NN	denotes	Figure
T8863	35703-35705	NN	denotes	6A
T8865	35706-35709	CC	denotes	and
T8866	35710-35712	NN	denotes	6B
T8867	35712-35713	-RRB-	denotes	)
T8868	35713-35714	.	denotes	.
T8869	35714-35899	sentence	denotes	Many osteoblasts line the newly formed cortical bone in the diaphyseal region (Figure 6C, blue arrow), and formation of a secondary ossification center has begun (Figure 6A, red star).
T8870	35715-35719	JJ	denotes	Many
T8871	35720-35731	NNS	denotes	osteoblasts
T8872	35732-35736	VBP	denotes	line
T8873	35737-35740	DT	denotes	the
T8875	35741-35746	RB	denotes	newly
T8876	35747-35753	VBN	denotes	formed
T8877	35754-35762	JJ	denotes	cortical
T8874	35763-35767	NN	denotes	bone
T8878	35768-35770	IN	denotes	in
T8879	35771-35774	DT	denotes	the
T8881	35775-35785	JJ	denotes	diaphyseal
T8880	35786-35792	NN	denotes	region
T8882	35793-35794	-LRB-	denotes	(
T8884	35794-35800	NN	denotes	Figure
T8885	35801-35803	NN	denotes	6C
T8886	35803-35805	,	denotes	,
T8887	35805-35809	JJ	denotes	blue
T8883	35810-35815	NN	denotes	arrow
T8888	35815-35816	-RRB-	denotes	)
T8889	35816-35818	,	denotes	,
T8890	35818-35821	CC	denotes	and
T8891	35822-35831	NN	denotes	formation
T8893	35832-35834	IN	denotes	of
T8894	35835-35836	DT	denotes	a
T8896	35837-35846	JJ	denotes	secondary
T8897	35847-35859	NN	denotes	ossification
T8895	35860-35866	NN	denotes	center
T8898	35867-35870	VBZ	denotes	has
T8892	35871-35876	VBN	denotes	begun
T8899	35877-35878	-LRB-	denotes	(
T8901	35878-35884	NN	denotes	Figure
T8902	35885-35887	NN	denotes	6A
T8903	35887-35889	,	denotes	,
T8904	35889-35892	JJ	denotes	red
T8900	35893-35897	NN	denotes	star
T8905	35897-35898	-RRB-	denotes	)
T8906	35898-35899	.	denotes	.
T8907	35899-36139	sentence	denotes	At 3 wks of age, formation of the secondary ossification center is complete (Figure 6D, red star), along with robust bone marrow formation (Figure 6D, bm) and deposition of trabecular bone (Figure 6E, pale blue tissue marked by red arrow).
T8908	35900-35902	IN	denotes	At
T8910	35903-35904	CD	denotes	3
T8911	35905-35908	NNS	denotes	wks
T8912	35909-35911	IN	denotes	of
T8913	35912-35915	NN	denotes	age
T8914	35915-35917	,	denotes	,
T8915	35917-35926	NN	denotes	formation
T8916	35927-35929	IN	denotes	of
T8917	35930-35933	DT	denotes	the
T8919	35934-35943	JJ	denotes	secondary
T8920	35944-35956	NN	denotes	ossification
T8918	35957-35963	NN	denotes	center
T8909	35964-35966	VBZ	denotes	is
T8921	35967-35975	JJ	denotes	complete
T8922	35976-35977	-LRB-	denotes	(
T8924	35977-35983	NN	denotes	Figure
T8925	35984-35986	NN	denotes	6D
T8926	35986-35988	,	denotes	,
T8927	35988-35991	JJ	denotes	red
T8923	35992-35996	NN	denotes	star
T8928	35996-35997	-RRB-	denotes	)
T8929	35997-35999	,	denotes	,
T8930	35999-36004	IN	denotes	along
T8931	36005-36009	IN	denotes	with
T8932	36010-36016	JJ	denotes	robust
T8934	36017-36021	NN	denotes	bone
T8935	36022-36028	NN	denotes	marrow
T8933	36029-36038	NN	denotes	formation
T8936	36039-36040	-LRB-	denotes	(
T8938	36040-36046	NN	denotes	Figure
T8939	36047-36049	NN	denotes	6D
T8940	36049-36051	,	denotes	,
T8937	36051-36053	NN	denotes	bm
T8941	36053-36054	-RRB-	denotes	)
T8942	36055-36058	CC	denotes	and
T8943	36059-36069	NN	denotes	deposition
T8944	36070-36072	IN	denotes	of
T8945	36073-36083	JJ	denotes	trabecular
T8946	36084-36088	NN	denotes	bone
T8947	36089-36090	-LRB-	denotes	(
T8949	36090-36096	NN	denotes	Figure
T8950	36097-36099	NN	denotes	6E
T8951	36099-36101	,	denotes	,
T8952	36101-36105	JJ	denotes	pale
T8953	36106-36110	JJ	denotes	blue
T8948	36111-36117	NN	denotes	tissue
T8954	36118-36124	VBN	denotes	marked
T8955	36125-36127	IN	denotes	by
T8956	36128-36131	JJ	denotes	red
T8957	36132-36137	NN	denotes	arrow
T8958	36137-36138	-RRB-	denotes	)
T8959	36138-36139	.	denotes	.
T8960	36139-36263	sentence	denotes	In stark contrast, bone formation is not observed in the absence of BMP2 and BMP4 at 1 or 3 wks after birth (Figure 6F–6J).
T8961	36140-36142	IN	denotes	In
T8963	36143-36148	JJ	denotes	stark
T8964	36149-36157	NN	denotes	contrast
T8965	36157-36159	,	denotes	,
T8966	36159-36163	NN	denotes	bone
T8967	36164-36173	NN	denotes	formation
T8968	36174-36176	VBZ	denotes	is
T8969	36177-36180	RB	denotes	not
T8962	36181-36189	VBN	denotes	observed
T8970	36190-36192	IN	denotes	in
T8971	36193-36196	DT	denotes	the
T8972	36197-36204	NN	denotes	absence
T8973	36205-36207	IN	denotes	of
T8974	36208-36212	NN	denotes	BMP2
T8975	36213-36216	CC	denotes	and
T8976	36217-36221	NN	denotes	BMP4
T8977	36222-36224	IN	denotes	at
T8978	36225-36226	CD	denotes	1
T8980	36227-36229	CC	denotes	or
T8981	36230-36231	CD	denotes	3
T8979	36232-36235	NNS	denotes	wks
T8982	36236-36241	IN	denotes	after
T8983	36242-36247	NN	denotes	birth
T8984	36248-36249	-LRB-	denotes	(
T8986	36249-36255	NN	denotes	Figure
T8985	36256-36258	NN	denotes	6F
T8987	36258-36259	SYM	denotes	–
T8988	36259-36261	NN	denotes	6J
T8989	36261-36262	-RRB-	denotes	)
T8990	36262-36263	.	denotes	.
T8991	36263-36566	sentence	denotes	The appearance of the skeletal elements in the double mutants remains similar to E17.5 structures seen in wild-type limbs (Figure 5E), and although there is a bone collar at the site of hypertrophic cartilage (Figure 6G), no bone marrow cavity, trabecular bone, or cortical bone is present (Figure 6F).
T8992	36264-36267	DT	denotes	The
T8993	36268-36278	NN	denotes	appearance
T8995	36279-36281	IN	denotes	of
T8996	36282-36285	DT	denotes	the
T8998	36286-36294	JJ	denotes	skeletal
T8997	36295-36303	NNS	denotes	elements
T8999	36304-36306	IN	denotes	in
T9000	36307-36310	DT	denotes	the
T9002	36311-36317	JJ	denotes	double
T9001	36318-36325	NNS	denotes	mutants
T8994	36326-36333	VBZ	denotes	remains
T9003	36334-36341	JJ	denotes	similar
T9004	36342-36344	IN	denotes	to
T9005	36345-36350	NN	denotes	E17.5
T9006	36351-36361	NNS	denotes	structures
T9007	36362-36366	VBN	denotes	seen
T9008	36367-36369	IN	denotes	in
T9009	36370-36374	JJ	denotes	wild
T9011	36374-36375	HYPH	denotes	-
T9010	36375-36379	NN	denotes	type
T9012	36380-36385	NNS	denotes	limbs
T9013	36386-36387	-LRB-	denotes	(
T9015	36387-36393	NN	denotes	Figure
T9014	36394-36396	NN	denotes	5E
T9016	36396-36397	-RRB-	denotes	)
T9017	36397-36399	,	denotes	,
T9018	36399-36402	CC	denotes	and
T9019	36403-36411	IN	denotes	although
T9021	36412-36417	EX	denotes	there
T9020	36418-36420	VBZ	denotes	is
T9023	36421-36422	DT	denotes	a
T9025	36423-36427	NN	denotes	bone
T9024	36428-36434	NN	denotes	collar
T9026	36435-36437	IN	denotes	at
T9027	36438-36441	DT	denotes	the
T9028	36442-36446	NN	denotes	site
T9029	36447-36449	IN	denotes	of
T9030	36450-36462	JJ	denotes	hypertrophic
T9031	36463-36472	NN	denotes	cartilage
T9032	36473-36474	-LRB-	denotes	(
T9034	36474-36480	NN	denotes	Figure
T9033	36481-36483	NN	denotes	6G
T9035	36483-36484	-RRB-	denotes	)
T9036	36484-36486	,	denotes	,
T9037	36486-36488	DT	denotes	no
T9039	36489-36493	NN	denotes	bone
T9040	36494-36500	NN	denotes	marrow
T9038	36501-36507	NN	denotes	cavity
T9041	36507-36509	,	denotes	,
T9042	36509-36519	JJ	denotes	trabecular
T9043	36520-36524	NN	denotes	bone
T9044	36524-36526	,	denotes	,
T9045	36526-36528	CC	denotes	or
T9046	36529-36537	JJ	denotes	cortical
T9047	36538-36542	NN	denotes	bone
T9022	36543-36545	VBZ	denotes	is
T9048	36546-36553	JJ	denotes	present
T9049	36554-36555	-LRB-	denotes	(
T9051	36555-36561	NN	denotes	Figure
T9050	36562-36564	NN	denotes	6F
T9052	36564-36565	-RRB-	denotes	)
T9053	36565-36566	.	denotes	.
T9054	36566-36897	sentence	denotes	Many osteoclasts have been recruited to the remaining mineralized cartilage and are actively resorbing this tissue (red asterisks, Figure 6I), so that by 3 wks, all the mineralized cartilage in the diaphyseal region has disappeared, leaving a void where bone formation should have occurred (Figure 6J, also see Figures 6L and S3).
T9055	36567-36571	JJ	denotes	Many
T9056	36572-36583	NNS	denotes	osteoclasts
T9058	36584-36588	VBP	denotes	have
T9059	36589-36593	VBN	denotes	been
T9057	36594-36603	VBN	denotes	recruited
T9060	36604-36606	IN	denotes	to
T9061	36607-36610	DT	denotes	the
T9063	36611-36620	VBG	denotes	remaining
T9064	36621-36632	VBN	denotes	mineralized
T9062	36633-36642	NN	denotes	cartilage
T9065	36643-36646	CC	denotes	and
T9066	36647-36650	VBP	denotes	are
T9068	36651-36659	RB	denotes	actively
T9067	36660-36669	VBG	denotes	resorbing
T9069	36670-36674	DT	denotes	this
T9070	36675-36681	NN	denotes	tissue
T9071	36682-36683	-LRB-	denotes	(
T9073	36683-36686	JJ	denotes	red
T9074	36687-36696	NNS	denotes	asterisks
T9075	36696-36698	,	denotes	,
T9076	36698-36704	NN	denotes	Figure
T9072	36705-36707	NN	denotes	6I
T9077	36707-36708	-RRB-	denotes	)
T9078	36708-36710	,	denotes	,
T9079	36710-36712	IN	denotes	so
T9081	36713-36717	IN	denotes	that
T9082	36718-36720	IN	denotes	by
T9083	36721-36722	CD	denotes	3
T9084	36723-36726	NNS	denotes	wks
T9085	36726-36728	,	denotes	,
T9086	36728-36731	PDT	denotes	all
T9088	36732-36735	DT	denotes	the
T9089	36736-36747	VBN	denotes	mineralized
T9087	36748-36757	NN	denotes	cartilage
T9090	36758-36760	IN	denotes	in
T9091	36761-36764	DT	denotes	the
T9093	36765-36775	JJ	denotes	diaphyseal
T9092	36776-36782	NN	denotes	region
T9094	36783-36786	VBZ	denotes	has
T9080	36787-36798	VBN	denotes	disappeared
T9095	36798-36800	,	denotes	,
T9096	36800-36807	VBG	denotes	leaving
T9097	36808-36809	DT	denotes	a
T9098	36810-36814	NN	denotes	void
T9099	36815-36820	WRB	denotes	where
T9101	36821-36825	NN	denotes	bone
T9102	36826-36835	NN	denotes	formation
T9103	36836-36842	MD	denotes	should
T9104	36843-36847	VB	denotes	have
T9100	36848-36856	VBN	denotes	occurred
T9105	36857-36858	-LRB-	denotes	(
T9107	36858-36864	NN	denotes	Figure
T9106	36865-36867	NN	denotes	6J
T9108	36867-36869	,	denotes	,
T9109	36869-36873	RB	denotes	also
T9110	36874-36877	VBP	denotes	see
T9111	36878-36885	NNS	denotes	Figures
T9112	36886-36888	NN	denotes	6L
T9113	36889-36892	CC	denotes	and
T9114	36893-36895	NN	denotes	S3
T9115	36895-36896	-RRB-	denotes	)
T9116	36896-36897	.	denotes	.
T9117	36897-37015	sentence	denotes	This void is eventually invaded by soft tissues and the adjacent muscle (marked s and m, respectively, in Figure 6J).
T9118	36898-36902	DT	denotes	This
T9119	36903-36907	NN	denotes	void
T9121	36908-36910	VBZ	denotes	is
T9122	36911-36921	RB	denotes	eventually
T9120	36922-36929	VBN	denotes	invaded
T9123	36930-36932	IN	denotes	by
T9124	36933-36937	JJ	denotes	soft
T9125	36938-36945	NNS	denotes	tissues
T9126	36946-36949	CC	denotes	and
T9127	36950-36953	DT	denotes	the
T9129	36954-36962	JJ	denotes	adjacent
T9128	36963-36969	NN	denotes	muscle
T9130	36970-36971	-LRB-	denotes	(
T9131	36971-36977	VBN	denotes	marked
T9132	36978-36979	NN	denotes	s
T9133	36980-36983	CC	denotes	and
T9134	36984-36985	NN	denotes	m
T9135	36985-36987	,	denotes	,
T9136	36987-36999	RB	denotes	respectively
T9137	36999-37001	,	denotes	,
T9138	37001-37003	IN	denotes	in
T9139	37004-37010	NN	denotes	Figure
T9140	37011-37013	NN	denotes	6J
T9141	37013-37014	-RRB-	denotes	)
T9142	37014-37015	.	denotes	.
T9143	37015-37179	sentence	denotes	The resorption of bone at 3 wks of age is clearly visible in the proximal femur of mutant animals where a portion of that bone is missing (Figure 6L, black arrow).
T9144	37016-37019	DT	denotes	The
T9145	37020-37030	NN	denotes	resorption
T9147	37031-37033	IN	denotes	of
T9148	37034-37038	NN	denotes	bone
T9149	37039-37041	IN	denotes	at
T9150	37042-37043	CD	denotes	3
T9151	37044-37047	NNS	denotes	wks
T9152	37048-37050	IN	denotes	of
T9153	37051-37054	NN	denotes	age
T9146	37055-37057	VBZ	denotes	is
T9154	37058-37065	RB	denotes	clearly
T9155	37066-37073	JJ	denotes	visible
T9156	37074-37076	IN	denotes	in
T9157	37077-37080	DT	denotes	the
T9159	37081-37089	JJ	denotes	proximal
T9158	37090-37095	NN	denotes	femur
T9160	37096-37098	IN	denotes	of
T9161	37099-37105	NN	denotes	mutant
T9162	37106-37113	NNS	denotes	animals
T9163	37114-37119	WRB	denotes	where
T9165	37120-37121	DT	denotes	a
T9166	37122-37129	NN	denotes	portion
T9167	37130-37132	IN	denotes	of
T9168	37133-37137	DT	denotes	that
T9169	37138-37142	NN	denotes	bone
T9164	37143-37145	VBZ	denotes	is
T9170	37146-37153	JJ	denotes	missing
T9171	37154-37155	-LRB-	denotes	(
T9173	37155-37161	NN	denotes	Figure
T9174	37162-37164	NN	denotes	6L
T9175	37164-37166	,	denotes	,
T9176	37166-37171	JJ	denotes	black
T9172	37172-37177	NN	denotes	arrow
T9177	37177-37178	-RRB-	denotes	)
T9178	37178-37179	.	denotes	.
T9179	37179-37365	sentence	denotes	The inability to complete osteoblast differentiation in the Bmp2C/C; Bmp4C/C; Prx1::cre mice results in strikingly defective limb morphology at 1 wk of age (compare Figure S3G and S3H).
T9180	37180-37183	DT	denotes	The
T9181	37184-37193	NN	denotes	inability
T9183	37194-37196	TO	denotes	to
T9184	37197-37205	VB	denotes	complete
T9185	37206-37216	NN	denotes	osteoblast
T9186	37217-37232	NN	denotes	differentiation
T9187	37233-37235	IN	denotes	in
T9188	37236-37239	DT	denotes	the
T9190	37240-37245	NN	denotes	Bmp2C
T9192	37245-37246	HYPH	denotes	/
T9191	37246-37247	NN	denotes	C
T9193	37247-37248	:	denotes	;
T9194	37249-37254	NN	denotes	Bmp4C
T9196	37254-37255	HYPH	denotes	/
T9195	37255-37256	NN	denotes	C
T9197	37256-37257	:	denotes	;
T9198	37258-37262	NN	denotes	Prx1
T9200	37262-37263	HYPH	denotes	:
T9201	37263-37264	HYPH	denotes	:
T9199	37264-37267	NN	denotes	cre
T9189	37268-37272	NNS	denotes	mice
T9182	37273-37280	VBZ	denotes	results
T9202	37281-37283	IN	denotes	in
T9203	37284-37294	RB	denotes	strikingly
T9204	37295-37304	JJ	denotes	defective
T9206	37305-37309	NN	denotes	limb
T9205	37310-37320	NN	denotes	morphology
T9207	37321-37323	IN	denotes	at
T9208	37324-37325	CD	denotes	1
T9209	37326-37328	NN	denotes	wk
T9210	37329-37331	IN	denotes	of
T9211	37332-37335	NN	denotes	age
T9212	37336-37337	-LRB-	denotes	(
T9213	37337-37344	VB	denotes	compare
T9214	37345-37351	NN	denotes	Figure
T9215	37352-37355	NN	denotes	S3G
T9216	37356-37359	CC	denotes	and
T9217	37360-37363	NN	denotes	S3H
T9218	37363-37364	-RRB-	denotes	)
T9219	37364-37365	.	denotes	.
T9220	37365-40645	sentence	denotes	Figure 5 In the Absence of BMP2 and BMP4, Osteogenesis Begins During Early Embryonic Development (A–D) Sagittal sections of forelimb from E15.5 embryos. (A and B) Stained with toluidine blue. (A) Distal ulna from wild-type embryo, (B) distal ulna/radius from Bmp2C/C; Bmp4C/C; Prx1::cre embryo. Mineralized cartilage is shown by dark purple and osteoid is shown by light blue (red asterisk). (C and D) Humeri sections were hybridized with digoxigenin labeled riboprobe for ColI. (C) Section of wild-type humerus from E15.5 embryo, (D) from Bmp2C/C; Bmp4C/C; Prx1::cre E15.5 embryo. (E–H) Sagittal sections of zeugopod from E17.5 embryos. (E and F) Stained with toluidine blue while G and H are stained for TRAP (brown stain, red arrow). (E and G) E17.5 proximal radius from wild-type embryo, (F and H) E17.5 proximal ulna/radius from Bmp2C/C; Bmp4C/C; Prx1::cre embryo. Although vascularization occurs in the absence of BMP2 and BMP4, few osteoclasts are observed in the mineralized cartilage in Bmp2C/C; Bmp4C/C; Prx1::cre mice. Please note all the panels other than (G) and (H) are photographed at ×20, while (G) and (H) were photographed at ×40. (I and J) Sections of newborn proximal radius (I) from control animal and newborn proximal ulna/radius (J) from Bmp2C/C; Bmp4C/C; Prx1::cre animal were stained with toluidine blue. Red arrow shows the bone marrow cavity in (E) and (I). Note that bone collar (red *) forms at the right time and place in the absence of BMP2 and BMP4. Also note that since Bmp2C/C; Bmp4C/C; Prx1::cre mice occasionally have only one bone in zeugopod and the elbow joint is occasionally fused, it is difficult to distinguish between the ulna and the radius. Figure 6 Defects in Bone Formation in the Absence of BMP2 and BMP4 Toluidine blue staining of sagittal sections of distal femurs. (A–E) Control femurs at 1 wk (A) and 3 wks (D) of age. Boxed areas in (A) are enlarged in (B) and (C). Boxed area in (D) is enlarged in (E). Blue arrows in (C) point to osteoblast cells lining the surface of cortical bone. Pink arrows in (G) show similar cells to those in (C) in Bmp2C/C; Bmp4C/C; Prx1::cre femurs. Red star in (A) and (D) marks the secondary ossification center. Pale blue–stained tissue in (E), marked by red arrows, is trabecular bone. bm, marks the bone marrow cavity in (D). (F–J) Bmp2C/C; Bmp4C/C; Prx1::cre femurs at 1 wk (F) and 3 wks (J) of age. Boxed areas in (F) are enlarged in (G), (H), and (I). (A), (D), (F), and (J) are shown with the same magnification. Note that there are defects in bone formation but no defect in osteoclast mediated bone resorption. Mineralized tissue in the mid shaft of femur is almost resorbed at 3 wks. (H) Fibroblast-like cells present in the bone shaft of Bmp2C/C; Bmp4C/C; Prx1::cre mouse. Red star in (I) shows the osteoclasts invading the Bmp2C/C; Bmp4C/C; Prx1::cre femurs at 1 wk of age. s and m mark soft tissues and muscle in (J), respectively. (K and L) Three-wk-old wild-type (K) and Bmp2C/C; Bmp4C/C; Prx1::cre (L) hindlimb skeletons stained with Alcian blue and Alizarin red. The black arrow shows the missing part of proximal femur. To determine if any osteoblast differentiation occurs in Bmp2C/C; Bmp4C/C; Prx1::cre forelimbs, we examined osteoblast-related gene expression (Figure 7).
T9221	40491-40493	TO	denotes	To
T9222	40494-40503	VB	denotes	determine
T9224	40504-40506	IN	denotes	if
T9226	40507-40510	DT	denotes	any
T9228	40511-40521	NN	denotes	osteoblast
T9227	40522-40537	NN	denotes	differentiation
T9225	40538-40544	VBZ	denotes	occurs
T9229	40545-40547	IN	denotes	in
T9230	40548-40553	NN	denotes	Bmp2C
T9232	40553-40554	HYPH	denotes	/
T9231	40554-40555	NN	denotes	C
T9234	40555-40556	:	denotes	;
T9235	40557-40562	NN	denotes	Bmp4C
T9237	40562-40563	HYPH	denotes	/
T9236	40563-40564	NN	denotes	C
T9238	40564-40565	:	denotes	;
T9239	40566-40570	NN	denotes	Prx1
T9241	40570-40571	HYPH	denotes	:
T9242	40571-40572	HYPH	denotes	:
T9240	40572-40575	NN	denotes	cre
T9233	40576-40585	NNS	denotes	forelimbs
T9243	40585-40587	,	denotes	,
T9244	40587-40589	PRP	denotes	we
T9223	40590-40598	VBD	denotes	examined
T9245	40599-40609	NN	denotes	osteoblast
T9247	40609-40610	HYPH	denotes	-
T9246	40610-40617	VBN	denotes	related
T9249	40618-40622	NN	denotes	gene
T9248	40623-40633	NN	denotes	expression
T9250	40634-40635	-LRB-	denotes	(
T9251	40635-40641	NN	denotes	Figure
T9252	40642-40643	CD	denotes	7
T9253	40643-40644	-RRB-	denotes	)
T9254	40644-40645	.	denotes	.
T9255	40645-40840	sentence	denotes	In the control mice, osteoblasts lining the surface of trabecular and cortical bone express type I collagen, runx2, and osterix genes at both 1 wk and 3 wks after birth (Figure 7A–7D and 7I–7L).
T9256	40646-40648	IN	denotes	In
T9258	40649-40652	DT	denotes	the
T9260	40653-40660	NN	denotes	control
T9259	40661-40665	NNS	denotes	mice
T9261	40665-40667	,	denotes	,
T9262	40667-40678	NNS	denotes	osteoblasts
T9263	40679-40685	VBG	denotes	lining
T9264	40686-40689	DT	denotes	the
T9265	40690-40697	NN	denotes	surface
T9266	40698-40700	IN	denotes	of
T9267	40701-40711	JJ	denotes	trabecular
T9269	40712-40715	CC	denotes	and
T9270	40716-40724	JJ	denotes	cortical
T9268	40725-40729	NN	denotes	bone
T9257	40730-40737	VBP	denotes	express
T9271	40738-40742	NN	denotes	type
T9273	40743-40744	CD	denotes	I
T9272	40745-40753	NN	denotes	collagen
T9274	40753-40755	,	denotes	,
T9275	40755-40760	NN	denotes	runx2
T9276	40760-40762	,	denotes	,
T9277	40762-40765	CC	denotes	and
T9278	40766-40773	NN	denotes	osterix
T9279	40774-40779	NNS	denotes	genes
T9280	40780-40782	IN	denotes	at
T9281	40783-40787	CC	denotes	both
T9283	40788-40789	CD	denotes	1
T9282	40790-40792	NN	denotes	wk
T9284	40793-40796	CC	denotes	and
T9285	40797-40798	CD	denotes	3
T9286	40799-40802	NNS	denotes	wks
T9287	40803-40808	IN	denotes	after
T9288	40809-40814	NN	denotes	birth
T9289	40815-40816	-LRB-	denotes	(
T9291	40816-40822	NN	denotes	Figure
T9290	40823-40825	NN	denotes	7A
T9292	40825-40826	SYM	denotes	–
T9293	40826-40828	NN	denotes	7D
T9294	40829-40832	CC	denotes	and
T9295	40833-40835	NN	denotes	7I
T9296	40835-40836	SYM	denotes	–
T9297	40836-40838	NN	denotes	7L
T9298	40838-40839	-RRB-	denotes	)
T9299	40839-40840	.	denotes	.
T9300	40840-40986	sentence	denotes	In the absence of BMP2 and BMP4, cells which are fibroblastic in appearance (Figure 6H) are found adjacent to the mineralized cartilage surfaces.
T9301	40841-40843	IN	denotes	In
T9303	40844-40847	DT	denotes	the
T9304	40848-40855	NN	denotes	absence
T9305	40856-40858	IN	denotes	of
T9306	40859-40863	NN	denotes	BMP2
T9307	40864-40867	CC	denotes	and
T9308	40868-40872	NN	denotes	BMP4
T9309	40872-40874	,	denotes	,
T9310	40874-40879	NNS	denotes	cells
T9311	40880-40885	WDT	denotes	which
T9312	40886-40889	VBP	denotes	are
T9313	40890-40902	JJ	denotes	fibroblastic
T9314	40903-40905	IN	denotes	in
T9315	40906-40916	NN	denotes	appearance
T9316	40917-40918	-LRB-	denotes	(
T9318	40918-40924	NN	denotes	Figure
T9317	40925-40927	NN	denotes	6H
T9319	40927-40928	-RRB-	denotes	)
T9320	40929-40932	VBP	denotes	are
T9302	40933-40938	VBN	denotes	found
T9321	40939-40947	JJ	denotes	adjacent
T9322	40948-40950	IN	denotes	to
T9323	40951-40954	DT	denotes	the
T9325	40955-40966	VBN	denotes	mineralized
T9326	40967-40976	NN	denotes	cartilage
T9324	40977-40985	NNS	denotes	surfaces
T9327	40985-40986	.	denotes	.
T9328	40986-41200	sentence	denotes	These cells express amounts of type I collagen and runx2 comparable to control osteoblasts, but their expression of osterix, while noticeable at 1 wk, becomes almost undetectable at 3 wks (Figure 7E–7H and 7M–7P).
T9329	40987-40992	DT	denotes	These
T9330	40993-40998	NNS	denotes	cells
T9331	40999-41006	VBP	denotes	express
T9332	41007-41014	NNS	denotes	amounts
T9333	41015-41017	IN	denotes	of
T9334	41018-41022	NN	denotes	type
T9336	41023-41024	CD	denotes	I
T9335	41025-41033	NN	denotes	collagen
T9337	41034-41037	CC	denotes	and
T9338	41038-41043	NN	denotes	runx2
T9339	41044-41054	JJ	denotes	comparable
T9340	41055-41057	IN	denotes	to
T9341	41058-41065	NN	denotes	control
T9342	41066-41077	NNS	denotes	osteoblasts
T9343	41077-41079	,	denotes	,
T9344	41079-41082	CC	denotes	but
T9345	41083-41088	PRP$	denotes	their
T9346	41089-41099	NN	denotes	expression
T9348	41100-41102	IN	denotes	of
T9349	41103-41110	NN	denotes	osterix
T9350	41110-41112	,	denotes	,
T9351	41112-41117	IN	denotes	while
T9352	41118-41128	JJ	denotes	noticeable
T9353	41129-41131	IN	denotes	at
T9354	41132-41133	CD	denotes	1
T9355	41134-41136	NN	denotes	wk
T9356	41136-41138	,	denotes	,
T9347	41138-41145	VBZ	denotes	becomes
T9357	41146-41152	RB	denotes	almost
T9358	41153-41165	JJ	denotes	undetectable
T9359	41166-41168	IN	denotes	at
T9360	41169-41170	CD	denotes	3
T9361	41171-41174	NNS	denotes	wks
T9362	41175-41176	-LRB-	denotes	(
T9364	41176-41182	NN	denotes	Figure
T9363	41183-41185	NN	denotes	7E
T9365	41185-41186	SYM	denotes	–
T9366	41186-41188	NN	denotes	7H
T9367	41189-41192	CC	denotes	and
T9368	41193-41195	NN	denotes	7M
T9369	41195-41196	SYM	denotes	–
T9370	41196-41198	NN	denotes	7P
T9371	41198-41199	-RRB-	denotes	)
T9372	41199-41200	.	denotes	.
T9373	41200-41415	sentence	denotes	These results allow us to conclude that the fibroblast-like cells resident in the bone shaft (Figure 6H) may be osteoprogenitor cells unable to differentiate into mature osteoblasts in the absence of BMP2 and BMP4.
T9374	41201-41206	DT	denotes	These
T9375	41207-41214	NNS	denotes	results
T9376	41215-41220	VBP	denotes	allow
T9377	41221-41223	PRP	denotes	us
T9379	41224-41226	TO	denotes	to
T9378	41227-41235	VB	denotes	conclude
T9380	41236-41240	IN	denotes	that
T9382	41241-41244	DT	denotes	the
T9384	41245-41255	NN	denotes	fibroblast
T9386	41255-41256	HYPH	denotes	-
T9385	41256-41260	JJ	denotes	like
T9383	41261-41266	NNS	denotes	cells
T9387	41267-41275	JJ	denotes	resident
T9388	41276-41278	IN	denotes	in
T9389	41279-41282	DT	denotes	the
T9391	41283-41287	NN	denotes	bone
T9390	41288-41293	NN	denotes	shaft
T9392	41294-41295	-LRB-	denotes	(
T9394	41295-41301	NN	denotes	Figure
T9393	41302-41304	NN	denotes	6H
T9395	41304-41305	-RRB-	denotes	)
T9396	41306-41309	MD	denotes	may
T9381	41310-41312	VB	denotes	be
T9397	41313-41328	NN	denotes	osteoprogenitor
T9398	41329-41334	NNS	denotes	cells
T9399	41335-41341	JJ	denotes	unable
T9400	41342-41344	TO	denotes	to
T9401	41345-41358	VB	denotes	differentiate
T9402	41359-41363	IN	denotes	into
T9403	41364-41370	JJ	denotes	mature
T9404	41371-41382	NNS	denotes	osteoblasts
T9405	41383-41385	IN	denotes	in
T9406	41386-41389	DT	denotes	the
T9407	41390-41397	NN	denotes	absence
T9408	41398-41400	IN	denotes	of
T9409	41401-41405	NN	denotes	BMP2
T9410	41406-41409	CC	denotes	and
T9411	41410-41414	NN	denotes	BMP4
T9412	41414-41415	.	denotes	.
T9476	41919-41924	VBG	denotes	Using
T9478	41925-41926	DT	denotes	a
T9480	41927-41934	JJ	denotes	genetic
T9479	41935-41943	NN	denotes	approach
T9481	41944-41946	TO	denotes	to
T9482	41947-41953	VB	denotes	remove
T9483	41954-41962	NN	denotes	activity
T9484	41963-41965	IN	denotes	of
T9485	41966-41975	JJ	denotes	different
T9487	41976-41979	NN	denotes	Bmp
T9486	41980-41985	NNS	denotes	genes
T9488	41986-41992	IN	denotes	during
T9489	41993-41997	NN	denotes	limb
T9490	41998-42009	NN	denotes	development
T9491	42009-42011	,	denotes	,
T9492	42011-42013	PRP	denotes	we
T9493	42014-42018	VBP	denotes	have
T9477	42019-42024	VBN	denotes	found
T9494	42025-42029	IN	denotes	that
T9496	42030-42034	NN	denotes	BMP2
T9497	42034-42036	,	denotes	,
T9498	42036-42040	NN	denotes	BMP4
T9499	42040-42042	,	denotes	,
T9500	42042-42045	CC	denotes	and
T9501	42046-42050	NN	denotes	BMP7
T9502	42051-42057	CC	denotes	either
T9504	42058-42070	RB	denotes	individually
T9505	42071-42073	CC	denotes	or
T9503	42074-42076	IN	denotes	in
T9506	42077-42088	NN	denotes	combination
T9507	42089-42092	VBP	denotes	are
T9508	42093-42096	RB	denotes	not
T9495	42097-42105	VBN	denotes	required
T9509	42106-42109	IN	denotes	for
T9510	42110-42123	NN	denotes	specification
T9511	42124-42126	IN	denotes	of
T9512	42127-42133	JJ	denotes	normal
T9514	42134-42139	NN	denotes	digit
T9513	42140-42150	NNS	denotes	identities
T9515	42150-42151	.	denotes	.
T9516	42151-42254	sentence	denotes	However, BMP2 and BMP4 are required in concert to promote condensation of the posterior digit anlagen.
T9517	42152-42159	RB	denotes	However
T9519	42159-42161	,	denotes	,
T9520	42161-42165	NN	denotes	BMP2
T9521	42166-42169	CC	denotes	and
T9522	42170-42174	NN	denotes	BMP4
T9523	42175-42178	VBP	denotes	are
T9518	42179-42187	VBN	denotes	required
T9524	42188-42190	IN	denotes	in
T9525	42191-42198	NN	denotes	concert
T9526	42199-42201	TO	denotes	to
T9527	42202-42209	VB	denotes	promote
T9528	42210-42222	NN	denotes	condensation
T9529	42223-42225	IN	denotes	of
T9530	42226-42229	DT	denotes	the
T9532	42230-42239	JJ	denotes	posterior
T9533	42240-42245	NN	denotes	digit
T9531	42246-42253	NNS	denotes	anlagen
T9534	42253-42254	.	denotes	.
T9535	42254-42419	sentence	denotes	We have also shown that chondrogenesis can be initiated and chondrogenic differentiation will take place even in the absence of both BMP2 and BMP4 or BMP2 and BMP7.
T9536	42255-42257	PRP	denotes	We
T9538	42258-42262	VBP	denotes	have
T9539	42263-42267	RB	denotes	also
T9537	42268-42273	VBN	denotes	shown
T9540	42274-42278	IN	denotes	that
T9542	42279-42293	NN	denotes	chondrogenesis
T9543	42294-42297	MD	denotes	can
T9544	42298-42300	VB	denotes	be
T9541	42301-42310	VBN	denotes	initiated
T9545	42311-42314	CC	denotes	and
T9546	42315-42327	JJ	denotes	chondrogenic
T9547	42328-42343	NN	denotes	differentiation
T9549	42344-42348	MD	denotes	will
T9548	42349-42353	VB	denotes	take
T9550	42354-42359	NN	denotes	place
T9551	42360-42364	RB	denotes	even
T9552	42365-42367	IN	denotes	in
T9553	42368-42371	DT	denotes	the
T9554	42372-42379	NN	denotes	absence
T9555	42380-42382	IN	denotes	of
T9556	42383-42387	CC	denotes	both
T9557	42388-42392	NN	denotes	BMP2
T9558	42393-42396	CC	denotes	and
T9559	42397-42401	NN	denotes	BMP4
T9560	42402-42404	CC	denotes	or
T9561	42405-42409	NN	denotes	BMP2
T9562	42410-42413	CC	denotes	and
T9563	42414-42418	NN	denotes	BMP7
T9564	42418-42419	.	denotes	.
T9565	42419-42506	sentence	denotes	On the other hand, BMP2 and BMP4 together are required for completion of osteogenesis.
T9566	42420-42422	IN	denotes	On
T9568	42423-42426	DT	denotes	the
T9570	42427-42432	JJ	denotes	other
T9569	42433-42437	NN	denotes	hand
T9571	42437-42439	,	denotes	,
T9572	42439-42443	NN	denotes	BMP2
T9573	42444-42447	CC	denotes	and
T9574	42448-42452	NN	denotes	BMP4
T9575	42453-42461	RB	denotes	together
T9576	42462-42465	VBP	denotes	are
T9567	42466-42474	VBN	denotes	required
T9577	42475-42478	IN	denotes	for
T9578	42479-42489	NN	denotes	completion
T9579	42490-42492	IN	denotes	of
T9580	42493-42505	NN	denotes	osteogenesis
T9581	42505-42506	.	denotes	.
T10021	42508-42511	NN	denotes	BMP
T10022	42512-42521	NN	denotes	Signaling
T10023	42522-42525	CC	denotes	and
T10024	42526-42533	NN	denotes	Pattern
T10025	42534-42543	NN	denotes	Formation
T10026	42543-42742	sentence	denotes	It has been proposed that BMP signaling may be involved in digit patterning as a secondary signal originating in the ZPA [7,11,43] or as a later mediator of interdigital patterning information [15].
T10027	42544-42546	PRP	denotes	It
T10029	42547-42550	VBZ	denotes	has
T10030	42551-42555	VBN	denotes	been
T10028	42556-42564	VBN	denotes	proposed
T10031	42565-42569	IN	denotes	that
T10033	42570-42573	NN	denotes	BMP
T10034	42574-42583	NN	denotes	signaling
T10035	42584-42587	MD	denotes	may
T10036	42588-42590	VB	denotes	be
T10032	42591-42599	VBN	denotes	involved
T10037	42600-42602	IN	denotes	in
T10038	42603-42608	NN	denotes	digit
T10039	42609-42619	NN	denotes	patterning
T10040	42620-42622	IN	denotes	as
T10041	42623-42624	DT	denotes	a
T10043	42625-42634	JJ	denotes	secondary
T10042	42635-42641	NN	denotes	signal
T10044	42642-42653	VBG	denotes	originating
T10045	42654-42656	IN	denotes	in
T10046	42657-42660	DT	denotes	the
T10047	42661-42664	NN	denotes	ZPA
T10048	42665-42666	-LRB-	denotes	[
T10050	42666-42667	CD	denotes	7
T10051	42667-42668	,	denotes	,
T10052	42668-42670	CD	denotes	11
T10053	42670-42671	,	denotes	,
T10049	42671-42673	CD	denotes	43
T10054	42673-42674	-RRB-	denotes	]
T10055	42675-42677	CC	denotes	or
T10056	42678-42680	IN	denotes	as
T10057	42681-42682	DT	denotes	a
T10059	42683-42688	JJ	denotes	later
T10058	42689-42697	NN	denotes	mediator
T10060	42698-42700	IN	denotes	of
T10061	42701-42713	JJ	denotes	interdigital
T10063	42714-42724	NN	denotes	patterning
T10062	42725-42736	NN	denotes	information
T10064	42737-42738	-LRB-	denotes	[
T10065	42738-42740	CD	denotes	15
T10066	42740-42741	-RRB-	denotes	]
T10067	42741-42742	.	denotes	.
T10068	42742-42945	sentence	denotes	In particular, BMP2, a secreted factor that is induced by SHH [8] in the posterior limb bud, has been suggested to act as a true morphogen secondary to SHH signaling to properly pattern the limb digits.
T10069	42743-42745	IN	denotes	In
T10071	42746-42756	JJ	denotes	particular
T10072	42756-42758	,	denotes	,
T10073	42758-42762	NN	denotes	BMP2
T10074	42762-42764	,	denotes	,
T10075	42764-42765	DT	denotes	a
T10077	42766-42774	VBN	denotes	secreted
T10076	42775-42781	NN	denotes	factor
T10078	42782-42786	WDT	denotes	that
T10080	42787-42789	VBZ	denotes	is
T10079	42790-42797	VBN	denotes	induced
T10081	42798-42800	IN	denotes	by
T10082	42801-42804	NN	denotes	SHH
T10083	42805-42806	-LRB-	denotes	[
T10084	42806-42807	CD	denotes	8
T10085	42807-42808	-RRB-	denotes	]
T10086	42809-42811	IN	denotes	in
T10087	42812-42815	DT	denotes	the
T10089	42816-42825	JJ	denotes	posterior
T10090	42826-42830	NN	denotes	limb
T10088	42831-42834	NN	denotes	bud
T10091	42834-42836	,	denotes	,
T10092	42836-42839	VBZ	denotes	has
T10093	42840-42844	VBN	denotes	been
T10070	42845-42854	VBN	denotes	suggested
T10094	42855-42857	TO	denotes	to
T10095	42858-42861	VB	denotes	act
T10096	42862-42864	IN	denotes	as
T10097	42865-42866	DT	denotes	a
T10099	42867-42871	JJ	denotes	true
T10098	42872-42881	NN	denotes	morphogen
T10100	42882-42891	JJ	denotes	secondary
T10101	42892-42894	IN	denotes	to
T10102	42895-42898	NN	denotes	SHH
T10103	42899-42908	NN	denotes	signaling
T10104	42909-42911	TO	denotes	to
T10106	42912-42920	RB	denotes	properly
T10105	42921-42928	VB	denotes	pattern
T10107	42929-42932	DT	denotes	the
T10109	42933-42937	NN	denotes	limb
T10108	42938-42944	NNS	denotes	digits
T10110	42944-42945	.	denotes	.
T10111	42945-43062	sentence	denotes	However, inactivation of Bmp2 during early limb bud development clearly does not cause any digit patterning defects.
T10112	42946-42953	RB	denotes	However
T10114	42953-42955	,	denotes	,
T10115	42955-42967	NN	denotes	inactivation
T10116	42968-42970	IN	denotes	of
T10117	42971-42975	NN	denotes	Bmp2
T10118	42976-42982	IN	denotes	during
T10119	42983-42988	JJ	denotes	early
T10121	42989-42993	NN	denotes	limb
T10122	42994-42997	NN	denotes	bud
T10120	42998-43009	NN	denotes	development
T10123	43010-43017	RB	denotes	clearly
T10124	43018-43022	VBZ	denotes	does
T10125	43023-43026	RB	denotes	not
T10113	43027-43032	VB	denotes	cause
T10126	43033-43036	DT	denotes	any
T10128	43037-43042	NN	denotes	digit
T10129	43043-43053	NN	denotes	patterning
T10127	43054-43061	NNS	denotes	defects
T10130	43061-43062	.	denotes	.
T10131	43062-43123	sentence	denotes	BMPs are known, in many cases, to function redundantly [44].
T10132	43063-43067	NNS	denotes	BMPs
T10134	43068-43071	VBP	denotes	are
T10133	43072-43077	VBN	denotes	known
T10135	43077-43079	,	denotes	,
T10136	43079-43081	IN	denotes	in
T10137	43082-43086	JJ	denotes	many
T10138	43087-43092	NNS	denotes	cases
T10139	43092-43094	,	denotes	,
T10140	43094-43096	TO	denotes	to
T10141	43097-43105	VB	denotes	function
T10142	43106-43117	RB	denotes	redundantly
T10143	43118-43119	-LRB-	denotes	[
T10144	43119-43121	CD	denotes	44
T10145	43121-43122	-RRB-	denotes	]
T10146	43122-43123	.	denotes	.
T10147	43123-43324	sentence	denotes	In considering potential secondary signals mediating ZPA activity, the most relevant family member to consider is BMP7 as, like BMP2 (and unlike BMP4), it is positively regulated by SHH signaling [7].
T10148	43124-43126	IN	denotes	In
T10150	43127-43138	VBG	denotes	considering
T10151	43139-43148	JJ	denotes	potential
T10153	43149-43158	JJ	denotes	secondary
T10152	43159-43166	NNS	denotes	signals
T10154	43167-43176	VBG	denotes	mediating
T10155	43177-43180	NN	denotes	ZPA
T10156	43181-43189	NN	denotes	activity
T10157	43189-43191	,	denotes	,
T10158	43191-43194	DT	denotes	the
T10160	43195-43199	RBS	denotes	most
T10161	43200-43208	JJ	denotes	relevant
T10162	43209-43215	NN	denotes	family
T10159	43216-43222	NN	denotes	member
T10163	43223-43225	TO	denotes	to
T10164	43226-43234	VB	denotes	consider
T10149	43235-43237	VBZ	denotes	is
T10165	43238-43242	NN	denotes	BMP7
T10166	43243-43245	IN	denotes	as
T10168	43245-43247	,	denotes	,
T10169	43247-43251	IN	denotes	like
T10170	43252-43256	NN	denotes	BMP2
T10171	43257-43258	-LRB-	denotes	(
T10172	43258-43261	CC	denotes	and
T10173	43262-43268	IN	denotes	unlike
T10174	43269-43273	NN	denotes	BMP4
T10175	43273-43274	-RRB-	denotes	)
T10176	43274-43276	,	denotes	,
T10177	43276-43278	PRP	denotes	it
T10178	43279-43281	VBZ	denotes	is
T10179	43282-43292	RB	denotes	positively
T10167	43293-43302	VBN	denotes	regulated
T10180	43303-43305	IN	denotes	by
T10181	43306-43309	NN	denotes	SHH
T10182	43310-43319	NN	denotes	signaling
T10183	43320-43321	-LRB-	denotes	[
T10184	43321-43322	CD	denotes	7
T10185	43322-43323	-RRB-	denotes	]
T10186	43323-43324	.	denotes	.
T10187	43324-43601	sentence	denotes	However, Bmp7 expression does not overlap with Bmp2 in the early mouse limb bud [45], and in any case, mice in which we conditionally knocked out Bmp2 in the Bmp7 −/− background do not show any obvious digit patterning defect other than the missing final phalanx in digit III.
T10188	43325-43332	RB	denotes	However
T10190	43332-43334	,	denotes	,
T10191	43334-43338	NN	denotes	Bmp7
T10192	43339-43349	NN	denotes	expression
T10193	43350-43354	VBZ	denotes	does
T10194	43355-43358	RB	denotes	not
T10189	43359-43366	VB	denotes	overlap
T10195	43367-43371	IN	denotes	with
T10196	43372-43376	NN	denotes	Bmp2
T10197	43377-43379	IN	denotes	in
T10198	43380-43383	DT	denotes	the
T10200	43384-43389	JJ	denotes	early
T10201	43390-43395	NN	denotes	mouse
T10202	43396-43400	NN	denotes	limb
T10199	43401-43404	NN	denotes	bud
T10203	43405-43406	-LRB-	denotes	[
T10204	43406-43408	CD	denotes	45
T10205	43408-43409	-RRB-	denotes	]
T10206	43409-43411	,	denotes	,
T10207	43411-43414	CC	denotes	and
T10208	43415-43417	IN	denotes	in
T10210	43418-43421	DT	denotes	any
T10211	43422-43426	NN	denotes	case
T10212	43426-43428	,	denotes	,
T10213	43428-43432	NNS	denotes	mice
T10214	43433-43435	IN	denotes	in
T10216	43436-43441	WDT	denotes	which
T10217	43442-43444	PRP	denotes	we
T10218	43445-43458	RB	denotes	conditionally
T10215	43459-43466	VBD	denotes	knocked
T10219	43467-43470	RP	denotes	out
T10220	43471-43475	NN	denotes	Bmp2
T10221	43476-43478	IN	denotes	in
T10222	43479-43482	DT	denotes	the
T10224	43483-43487	NN	denotes	Bmp7
T10225	43488-43489	SYM	denotes	−
T10226	43489-43490	HYPH	denotes	/
T10227	43490-43491	SYM	denotes	−
T10223	43492-43502	NN	denotes	background
T10228	43503-43505	VBP	denotes	do
T10229	43506-43509	RB	denotes	not
T10209	43510-43514	VB	denotes	show
T10230	43515-43518	DT	denotes	any
T10232	43519-43526	JJ	denotes	obvious
T10233	43527-43532	NN	denotes	digit
T10234	43533-43543	NN	denotes	patterning
T10231	43544-43550	NN	denotes	defect
T10235	43551-43556	JJ	denotes	other
T10236	43557-43561	IN	denotes	than
T10237	43562-43565	DT	denotes	the
T10239	43566-43573	JJ	denotes	missing
T10240	43574-43579	JJ	denotes	final
T10238	43580-43587	NN	denotes	phalanx
T10241	43588-43590	IN	denotes	in
T10242	43591-43596	NN	denotes	digit
T10243	43597-43600	CD	denotes	III
T10244	43600-43601	.	denotes	.
T10245	43601-43752	sentence	denotes	Although still poorly understood, the final phalanges form at the tip of every digit through a process distinct from the more proximal phalanges [38].
T10246	43602-43610	IN	denotes	Although
T10248	43611-43616	RB	denotes	still
T10249	43617-43623	RB	denotes	poorly
T10247	43624-43634	VBN	denotes	understood
T10251	43634-43636	,	denotes	,
T10252	43636-43639	DT	denotes	the
T10254	43640-43645	JJ	denotes	final
T10253	43646-43655	NNS	denotes	phalanges
T10250	43656-43660	VBP	denotes	form
T10255	43661-43663	IN	denotes	at
T10256	43664-43667	DT	denotes	the
T10257	43668-43671	NN	denotes	tip
T10258	43672-43674	IN	denotes	of
T10259	43675-43680	DT	denotes	every
T10260	43681-43686	NN	denotes	digit
T10261	43687-43694	IN	denotes	through
T10262	43695-43696	DT	denotes	a
T10263	43697-43704	NN	denotes	process
T10264	43705-43713	JJ	denotes	distinct
T10265	43714-43718	IN	denotes	from
T10266	43719-43722	DT	denotes	the
T10268	43723-43727	RBR	denotes	more
T10269	43728-43736	JJ	denotes	proximal
T10267	43737-43746	NNS	denotes	phalanges
T10270	43747-43748	-LRB-	denotes	[
T10271	43748-43750	CD	denotes	38
T10272	43750-43751	-RRB-	denotes	]
T10273	43751-43752	.	denotes	.
T10274	43752-43802	sentence	denotes	Our data implicate BMP signaling in this process.
T10275	43753-43756	PRP$	denotes	Our
T10276	43757-43761	NNS	denotes	data
T10277	43762-43771	VBP	denotes	implicate
T10278	43772-43775	NN	denotes	BMP
T10279	43776-43785	NN	denotes	signaling
T10280	43786-43788	IN	denotes	in
T10281	43789-43793	DT	denotes	this
T10282	43794-43801	NN	denotes	process
T10283	43801-43802	.	denotes	.
T10284	43802-44005	sentence	denotes	At later stages, BMP signaling has been suggested to play a role in mediating the transfer of positional information from the interdigital mesenchyme to specify the identity of the adjacent digits [15].
T10285	43803-43805	IN	denotes	At
T10287	43806-43811	JJ	denotes	later
T10288	43812-43818	NNS	denotes	stages
T10289	43818-43820	,	denotes	,
T10290	43820-43823	NN	denotes	BMP
T10291	43824-43833	NN	denotes	signaling
T10292	43834-43837	VBZ	denotes	has
T10293	43838-43842	VBN	denotes	been
T10286	43843-43852	VBN	denotes	suggested
T10294	43853-43855	TO	denotes	to
T10295	43856-43860	VB	denotes	play
T10296	43861-43862	DT	denotes	a
T10297	43863-43867	NN	denotes	role
T10298	43868-43870	IN	denotes	in
T10299	43871-43880	VBG	denotes	mediating
T10300	43881-43884	DT	denotes	the
T10301	43885-43893	NN	denotes	transfer
T10302	43894-43896	IN	denotes	of
T10303	43897-43907	JJ	denotes	positional
T10304	43908-43919	NN	denotes	information
T10305	43920-43924	IN	denotes	from
T10306	43925-43928	DT	denotes	the
T10308	43929-43941	JJ	denotes	interdigital
T10307	43942-43952	NN	denotes	mesenchyme
T10309	43953-43955	TO	denotes	to
T10310	43956-43963	VB	denotes	specify
T10311	43964-43967	DT	denotes	the
T10312	43968-43976	NN	denotes	identity
T10313	43977-43979	IN	denotes	of
T10314	43980-43983	DT	denotes	the
T10316	43984-43992	JJ	denotes	adjacent
T10315	43993-43999	NNS	denotes	digits
T10317	44000-44001	-LRB-	denotes	[
T10318	44001-44003	CD	denotes	15
T10319	44003-44004	-RRB-	denotes	]
T10320	44004-44005	.	denotes	.
T10321	44005-44242	sentence	denotes	Application of the BMP antagonist noggin to the interdigital tissue at the time of digit condensation results in loss of phalanges and a resultant pattern that can be interpreted as an anterior homeotic transformation in digit identity.
T10322	44006-44017	NN	denotes	Application
T10324	44018-44020	IN	denotes	of
T10325	44021-44024	DT	denotes	the
T10327	44025-44028	NN	denotes	BMP
T10328	44029-44039	NN	denotes	antagonist
T10326	44040-44046	NN	denotes	noggin
T10329	44047-44049	IN	denotes	to
T10330	44050-44053	DT	denotes	the
T10332	44054-44066	JJ	denotes	interdigital
T10331	44067-44073	NN	denotes	tissue
T10333	44074-44076	IN	denotes	at
T10334	44077-44080	DT	denotes	the
T10335	44081-44085	NN	denotes	time
T10336	44086-44088	IN	denotes	of
T10337	44089-44094	NN	denotes	digit
T10338	44095-44107	NN	denotes	condensation
T10323	44108-44115	VBZ	denotes	results
T10339	44116-44118	IN	denotes	in
T10340	44119-44123	NN	denotes	loss
T10341	44124-44126	IN	denotes	of
T10342	44127-44136	NNS	denotes	phalanges
T10343	44137-44140	CC	denotes	and
T10344	44141-44142	DT	denotes	a
T10346	44143-44152	JJ	denotes	resultant
T10345	44153-44160	NN	denotes	pattern
T10347	44161-44165	WDT	denotes	that
T10349	44166-44169	MD	denotes	can
T10350	44170-44172	VB	denotes	be
T10348	44173-44184	VBN	denotes	interpreted
T10351	44185-44187	IN	denotes	as
T10352	44188-44190	DT	denotes	an
T10354	44191-44199	JJ	denotes	anterior
T10355	44200-44208	JJ	denotes	homeotic
T10353	44209-44223	NN	denotes	transformation
T10356	44224-44226	IN	denotes	in
T10357	44227-44232	NN	denotes	digit
T10358	44233-44241	NN	denotes	identity
T10359	44241-44242	.	denotes	.
T10360	44242-44554	sentence	denotes	However, increasing BMP concentrations interdigitally with exogenous protein does not result in the reciprocal posterior transformations, and the noggin experiments can also be interpreted simply as a block in the condensation of the primordia of distal phalanges when BMP activity is completely abolished [38].
T10361	44243-44250	RB	denotes	However
T10363	44250-44252	,	denotes	,
T10364	44252-44262	VBG	denotes	increasing
T10365	44263-44266	NN	denotes	BMP
T10366	44267-44281	NNS	denotes	concentrations
T10367	44282-44296	RB	denotes	interdigitally
T10368	44297-44301	IN	denotes	with
T10369	44302-44311	JJ	denotes	exogenous
T10370	44312-44319	NN	denotes	protein
T10371	44320-44324	VBZ	denotes	does
T10372	44325-44328	RB	denotes	not
T10362	44329-44335	VB	denotes	result
T10373	44336-44338	IN	denotes	in
T10374	44339-44342	DT	denotes	the
T10376	44343-44353	JJ	denotes	reciprocal
T10377	44354-44363	JJ	denotes	posterior
T10375	44364-44379	NNS	denotes	transformations
T10378	44379-44381	,	denotes	,
T10379	44381-44384	CC	denotes	and
T10380	44385-44388	DT	denotes	the
T10382	44389-44395	NN	denotes	noggin
T10381	44396-44407	NNS	denotes	experiments
T10384	44408-44411	MD	denotes	can
T10385	44412-44416	RB	denotes	also
T10386	44417-44419	VB	denotes	be
T10383	44420-44431	VBN	denotes	interpreted
T10387	44432-44438	RB	denotes	simply
T10388	44439-44441	IN	denotes	as
T10389	44442-44443	DT	denotes	a
T10390	44444-44449	NN	denotes	block
T10391	44450-44452	IN	denotes	in
T10392	44453-44456	DT	denotes	the
T10393	44457-44469	NN	denotes	condensation
T10394	44470-44472	IN	denotes	of
T10395	44473-44476	DT	denotes	the
T10396	44477-44486	NNS	denotes	primordia
T10397	44487-44489	IN	denotes	of
T10398	44490-44496	JJ	denotes	distal
T10399	44497-44506	NNS	denotes	phalanges
T10400	44507-44511	WRB	denotes	when
T10402	44512-44515	NN	denotes	BMP
T10403	44516-44524	NN	denotes	activity
T10404	44525-44527	VBZ	denotes	is
T10405	44528-44538	RB	denotes	completely
T10401	44539-44548	VBN	denotes	abolished
T10406	44549-44550	-LRB-	denotes	[
T10407	44550-44552	CD	denotes	38
T10408	44552-44553	-RRB-	denotes	]
T10409	44553-44554	.	denotes	.
T10410	44554-44695	sentence	denotes	Decreasing the level of interdigital BMP signaling, without abolishing it, provides an opportunity for differentiating between these models.
T10411	44555-44565	VBG	denotes	Decreasing
T10413	44566-44569	DT	denotes	the
T10414	44570-44575	NN	denotes	level
T10415	44576-44578	IN	denotes	of
T10416	44579-44591	JJ	denotes	interdigital
T10418	44592-44595	NN	denotes	BMP
T10417	44596-44605	NN	denotes	signaling
T10419	44605-44607	,	denotes	,
T10420	44607-44614	IN	denotes	without
T10421	44615-44625	VBG	denotes	abolishing
T10422	44626-44628	PRP	denotes	it
T10423	44628-44630	,	denotes	,
T10412	44630-44638	VBZ	denotes	provides
T10424	44639-44641	DT	denotes	an
T10425	44642-44653	NN	denotes	opportunity
T10426	44654-44657	IN	denotes	for
T10427	44658-44673	VBG	denotes	differentiating
T10428	44674-44681	IN	denotes	between
T10429	44682-44687	DT	denotes	these
T10430	44688-44694	NNS	denotes	models
T10431	44694-44695	.	denotes	.
T10432	44695-44840	sentence	denotes	If BMP signaling were only required as a permissive factor for chondrogenesis, partial reduction in the level of signaling might have no effect.
T10433	44696-44698	IN	denotes	If
T10435	44699-44702	NN	denotes	BMP
T10436	44703-44712	NN	denotes	signaling
T10437	44713-44717	VBD	denotes	were
T10438	44718-44722	RB	denotes	only
T10434	44723-44731	VBN	denotes	required
T10440	44732-44734	IN	denotes	as
T10441	44735-44736	DT	denotes	a
T10443	44737-44747	JJ	denotes	permissive
T10442	44748-44754	NN	denotes	factor
T10444	44755-44758	IN	denotes	for
T10445	44759-44773	NN	denotes	chondrogenesis
T10446	44773-44775	,	denotes	,
T10447	44775-44782	JJ	denotes	partial
T10448	44783-44792	NN	denotes	reduction
T10449	44793-44795	IN	denotes	in
T10450	44796-44799	DT	denotes	the
T10451	44800-44805	NN	denotes	level
T10452	44806-44808	IN	denotes	of
T10453	44809-44818	NN	denotes	signaling
T10454	44819-44824	MD	denotes	might
T10439	44825-44829	VB	denotes	have
T10455	44830-44832	DT	denotes	no
T10456	44833-44839	NN	denotes	effect
T10457	44839-44840	.	denotes	.
T10458	44840-45035	sentence	denotes	However, if levels of BMP signaling were instructive in terms of digit identities, then a substantial decrease in the amount of BMP ligand present should cause transformations in digit identity.
T10459	44841-44848	RB	denotes	However
T10461	44848-44850	,	denotes	,
T10462	44850-44852	IN	denotes	if
T10464	44853-44859	NNS	denotes	levels
T10465	44860-44862	IN	denotes	of
T10466	44863-44866	NN	denotes	BMP
T10467	44867-44876	NN	denotes	signaling
T10463	44877-44881	VBD	denotes	were
T10468	44882-44893	JJ	denotes	instructive
T10469	44894-44896	IN	denotes	in
T10470	44897-44902	NNS	denotes	terms
T10471	44903-44905	IN	denotes	of
T10472	44906-44911	NN	denotes	digit
T10473	44912-44922	NNS	denotes	identities
T10474	44922-44924	,	denotes	,
T10475	44924-44928	RB	denotes	then
T10476	44929-44930	DT	denotes	a
T10478	44931-44942	JJ	denotes	substantial
T10477	44943-44951	NN	denotes	decrease
T10479	44952-44954	IN	denotes	in
T10480	44955-44958	DT	denotes	the
T10481	44959-44965	NN	denotes	amount
T10482	44966-44968	IN	denotes	of
T10483	44969-44972	NN	denotes	BMP
T10484	44973-44979	NN	denotes	ligand
T10485	44980-44987	JJ	denotes	present
T10486	44988-44994	MD	denotes	should
T10460	44995-45000	VB	denotes	cause
T10487	45001-45016	NNS	denotes	transformations
T10488	45017-45019	IN	denotes	in
T10489	45020-45025	NN	denotes	digit
T10490	45026-45034	NN	denotes	identity
T10491	45034-45035	.	denotes	.
T10492	45035-45153	sentence	denotes	This is not what is observed, either in limbs deficient in both BMP2 and BMP4 or in those deficient in BMP2 and BMP7.
T10493	45036-45040	DT	denotes	This
T10494	45041-45043	VBZ	denotes	is
T10495	45044-45047	RB	denotes	not
T10496	45048-45052	WP	denotes	what
T10498	45053-45055	VBZ	denotes	is
T10497	45056-45064	VBN	denotes	observed
T10499	45064-45066	,	denotes	,
T10500	45066-45072	CC	denotes	either
T10501	45073-45075	IN	denotes	in
T10502	45076-45081	NNS	denotes	limbs
T10503	45082-45091	JJ	denotes	deficient
T10504	45092-45094	IN	denotes	in
T10505	45095-45099	CC	denotes	both
T10506	45100-45104	NN	denotes	BMP2
T10507	45105-45108	CC	denotes	and
T10508	45109-45113	NN	denotes	BMP4
T10509	45114-45116	CC	denotes	or
T10510	45117-45119	IN	denotes	in
T10511	45120-45125	DT	denotes	those
T10512	45126-45135	JJ	denotes	deficient
T10513	45136-45138	IN	denotes	in
T10514	45139-45143	NN	denotes	BMP2
T10515	45144-45147	CC	denotes	and
T10516	45148-45152	NN	denotes	BMP7
T10517	45152-45153	.	denotes	.
T10518	45153-45290	sentence	denotes	It should be noted that the interdigital expression domains form several days after recombination of the floxed BMP alleles is complete.
T10519	45154-45156	PRP	denotes	It
T10521	45157-45163	MD	denotes	should
T10522	45164-45166	VB	denotes	be
T10520	45167-45172	VBN	denotes	noted
T10523	45173-45177	IN	denotes	that
T10525	45178-45181	DT	denotes	the
T10527	45182-45194	JJ	denotes	interdigital
T10528	45195-45205	NN	denotes	expression
T10526	45206-45213	NNS	denotes	domains
T10524	45214-45218	VBP	denotes	form
T10529	45219-45226	JJ	denotes	several
T10530	45227-45231	NNS	denotes	days
T10532	45232-45237	IN	denotes	after
T10533	45238-45251	NN	denotes	recombination
T10534	45252-45254	IN	denotes	of
T10535	45255-45258	DT	denotes	the
T10537	45259-45265	VBN	denotes	floxed
T10538	45266-45269	NN	denotes	BMP
T10536	45270-45277	NNS	denotes	alleles
T10531	45278-45280	VBZ	denotes	is
T10539	45281-45289	JJ	denotes	complete
T10540	45289-45290	.	denotes	.
T10541	45290-45457	sentence	denotes	Moreover, there is no detectable compensatory upregulation of BMP7 in the interdigital mesenchyme of the BMP2, BMP4–deficient limbs (compare Figure 4G and Figure 4H).
T10542	45291-45299	RB	denotes	Moreover
T10544	45299-45301	,	denotes	,
T10545	45301-45306	EX	denotes	there
T10543	45307-45309	VBZ	denotes	is
T10546	45310-45312	DT	denotes	no
T10548	45313-45323	JJ	denotes	detectable
T10549	45324-45336	JJ	denotes	compensatory
T10547	45337-45349	NN	denotes	upregulation
T10550	45350-45352	IN	denotes	of
T10551	45353-45357	NN	denotes	BMP7
T10552	45358-45360	IN	denotes	in
T10553	45361-45364	DT	denotes	the
T10555	45365-45377	JJ	denotes	interdigital
T10554	45378-45388	NN	denotes	mesenchyme
T10556	45389-45391	IN	denotes	of
T10557	45392-45395	DT	denotes	the
T10559	45396-45400	NN	denotes	BMP2
T10561	45400-45402	,	denotes	,
T10560	45402-45406	NN	denotes	BMP4
T10563	45406-45407	HYPH	denotes	–
T10562	45407-45416	JJ	denotes	deficient
T10558	45417-45422	NNS	denotes	limbs
T10564	45423-45424	-LRB-	denotes	(
T10565	45424-45431	VB	denotes	compare
T10566	45432-45438	NN	denotes	Figure
T10567	45439-45441	NN	denotes	4G
T10568	45442-45445	CC	denotes	and
T10569	45446-45452	NN	denotes	Figure
T10570	45453-45455	NN	denotes	4H
T10571	45455-45456	-RRB-	denotes	)
T10572	45456-45457	.	denotes	.
T10573	45457-45638	sentence	denotes	Thus, we conclude that BMP signaling is not likely to be responsible in a quantitative fashion for the instructive role of interdigital mesenchyme in establishing digit identities.
T10574	45458-45462	RB	denotes	Thus
T10576	45462-45464	,	denotes	,
T10577	45464-45466	PRP	denotes	we
T10575	45467-45475	VBP	denotes	conclude
T10578	45476-45480	IN	denotes	that
T10580	45481-45484	NN	denotes	BMP
T10581	45485-45494	NN	denotes	signaling
T10579	45495-45497	VBZ	denotes	is
T10582	45498-45501	RB	denotes	not
T10583	45502-45508	JJ	denotes	likely
T10584	45509-45511	TO	denotes	to
T10585	45512-45514	VB	denotes	be
T10586	45515-45526	JJ	denotes	responsible
T10587	45527-45529	IN	denotes	in
T10588	45530-45531	DT	denotes	a
T10590	45532-45544	JJ	denotes	quantitative
T10589	45545-45552	NN	denotes	fashion
T10591	45553-45556	IN	denotes	for
T10592	45557-45560	DT	denotes	the
T10594	45561-45572	JJ	denotes	instructive
T10593	45573-45577	NN	denotes	role
T10595	45578-45580	IN	denotes	of
T10596	45581-45593	JJ	denotes	interdigital
T10597	45594-45604	NN	denotes	mesenchyme
T10598	45605-45607	IN	denotes	in
T10599	45608-45620	VBG	denotes	establishing
T10600	45621-45626	NN	denotes	digit
T10601	45627-45637	NNS	denotes	identities
T10602	45637-45638	.	denotes	.
T10603	45638-45940	sentence	denotes	However, in the Bmp2C/C; Bmp4C/C; Prx1::cre forelimbs, there is a complete loss of posterior digit ray condensations, presumably because the total level of BMP signaling falls below the threshold for initiation of condensation, as previously seen in cases where BMP antagonists have been used [27,28].
T10604	45639-45646	RB	denotes	However
T10606	45646-45648	,	denotes	,
T10607	45648-45650	IN	denotes	in
T10608	45651-45654	DT	denotes	the
T10610	45655-45660	NN	denotes	Bmp2C
T10612	45660-45661	HYPH	denotes	/
T10611	45661-45662	NN	denotes	C
T10613	45662-45663	:	denotes	;
T10614	45664-45669	NN	denotes	Bmp4C
T10616	45669-45670	HYPH	denotes	/
T10615	45670-45671	NN	denotes	C
T10617	45671-45672	:	denotes	;
T10618	45673-45677	NN	denotes	Prx1
T10620	45677-45678	HYPH	denotes	:
T10621	45678-45679	HYPH	denotes	:
T10619	45679-45682	NN	denotes	cre
T10609	45683-45692	NNS	denotes	forelimbs
T10622	45692-45694	,	denotes	,
T10623	45694-45699	EX	denotes	there
T10605	45700-45702	VBZ	denotes	is
T10624	45703-45704	DT	denotes	a
T10626	45705-45713	JJ	denotes	complete
T10625	45714-45718	NN	denotes	loss
T10627	45719-45721	IN	denotes	of
T10628	45722-45731	JJ	denotes	posterior
T10629	45732-45737	NN	denotes	digit
T10631	45738-45741	NN	denotes	ray
T10630	45742-45755	NNS	denotes	condensations
T10632	45755-45757	,	denotes	,
T10633	45757-45767	RB	denotes	presumably
T10635	45768-45775	IN	denotes	because
T10636	45776-45779	DT	denotes	the
T10638	45780-45785	JJ	denotes	total
T10637	45786-45791	NN	denotes	level
T10639	45792-45794	IN	denotes	of
T10640	45795-45798	NN	denotes	BMP
T10641	45799-45808	NN	denotes	signaling
T10634	45809-45814	VBZ	denotes	falls
T10642	45815-45820	IN	denotes	below
T10643	45821-45824	DT	denotes	the
T10644	45825-45834	NN	denotes	threshold
T10645	45835-45838	IN	denotes	for
T10646	45839-45849	NN	denotes	initiation
T10647	45850-45852	IN	denotes	of
T10648	45853-45865	NN	denotes	condensation
T10649	45865-45867	,	denotes	,
T10650	45867-45869	IN	denotes	as
T10652	45870-45880	RB	denotes	previously
T10651	45881-45885	VBN	denotes	seen
T10653	45886-45888	IN	denotes	in
T10654	45889-45894	NNS	denotes	cases
T10655	45895-45900	WRB	denotes	where
T10657	45901-45904	NN	denotes	BMP
T10658	45905-45916	NNS	denotes	antagonists
T10659	45917-45921	VBP	denotes	have
T10660	45922-45926	VBN	denotes	been
T10656	45927-45931	VBN	denotes	used
T10661	45932-45933	-LRB-	denotes	[
T10663	45933-45935	CD	denotes	27
T10664	45935-45936	,	denotes	,
T10662	45936-45938	CD	denotes	28
T10665	45938-45939	-RRB-	denotes	]
T10666	45939-45940	.	denotes	.
T10667	45940-46080	sentence	denotes	It is worth noting that the defects in chondrogenesis seen in the BMP2, BMP4–deficient limbs are much more severe proximally than distally.
T10668	45941-45943	PRP	denotes	It
T10669	45944-45946	VBZ	denotes	is
T10670	45947-45952	JJ	denotes	worth
T10671	45953-45959	VBG	denotes	noting
T10672	45960-45964	IN	denotes	that
T10674	45965-45968	DT	denotes	the
T10675	45969-45976	NNS	denotes	defects
T10676	45977-45979	IN	denotes	in
T10677	45980-45994	NN	denotes	chondrogenesis
T10678	45995-45999	VBN	denotes	seen
T10679	46000-46002	IN	denotes	in
T10680	46003-46006	DT	denotes	the
T10682	46007-46011	NN	denotes	BMP2
T10684	46011-46013	,	denotes	,
T10683	46013-46017	NN	denotes	BMP4
T10686	46017-46018	HYPH	denotes	–
T10685	46018-46027	JJ	denotes	deficient
T10681	46028-46033	NNS	denotes	limbs
T10673	46034-46037	VBP	denotes	are
T10687	46038-46042	RB	denotes	much
T10688	46043-46047	RBR	denotes	more
T10689	46048-46054	JJ	denotes	severe
T10690	46055-46065	RB	denotes	proximally
T10691	46066-46070	IN	denotes	than
T10692	46071-46079	RB	denotes	distally
T10693	46079-46080	.	denotes	.
T10694	46080-46186	sentence	denotes	This is particularly significant because proximal elements condense and differentiate before distal ones.
T10695	46081-46085	DT	denotes	This
T10696	46086-46088	VBZ	denotes	is
T10697	46089-46101	RB	denotes	particularly
T10698	46102-46113	JJ	denotes	significant
T10699	46114-46121	IN	denotes	because
T10701	46122-46130	JJ	denotes	proximal
T10702	46131-46139	NNS	denotes	elements
T10700	46140-46148	VBP	denotes	condense
T10703	46149-46152	CC	denotes	and
T10704	46153-46166	VBP	denotes	differentiate
T10705	46167-46173	IN	denotes	before
T10706	46174-46180	JJ	denotes	distal
T10707	46181-46185	NNS	denotes	ones
T10708	46185-46186	.	denotes	.
T10709	46186-46427	sentence	denotes	Thus, if the conditional alleles were partially recombined at early stages and only completely recombined at later stages, it is the distal elements, formed after greater recombination has taken place, that should be more severely affected.
T10710	46187-46191	RB	denotes	Thus
T10712	46191-46193	,	denotes	,
T10713	46193-46195	IN	denotes	if
T10715	46196-46199	DT	denotes	the
T10717	46200-46211	JJ	denotes	conditional
T10716	46212-46219	NNS	denotes	alleles
T10718	46220-46224	VBD	denotes	were
T10719	46225-46234	RB	denotes	partially
T10714	46235-46245	VBN	denotes	recombined
T10720	46246-46248	IN	denotes	at
T10721	46249-46254	JJ	denotes	early
T10722	46255-46261	NNS	denotes	stages
T10723	46262-46265	CC	denotes	and
T10724	46266-46270	RB	denotes	only
T10725	46271-46281	RB	denotes	completely
T10726	46282-46292	VBN	denotes	recombined
T10727	46293-46295	IN	denotes	at
T10728	46296-46301	JJ	denotes	later
T10729	46302-46308	NNS	denotes	stages
T10730	46308-46310	,	denotes	,
T10731	46310-46312	PRP	denotes	it
T10711	46313-46315	VBZ	denotes	is
T10732	46316-46319	DT	denotes	the
T10734	46320-46326	JJ	denotes	distal
T10733	46327-46335	NNS	denotes	elements
T10735	46335-46337	,	denotes	,
T10736	46337-46343	VBN	denotes	formed
T10737	46344-46349	IN	denotes	after
T10739	46350-46357	JJR	denotes	greater
T10740	46358-46371	NN	denotes	recombination
T10741	46372-46375	VBZ	denotes	has
T10738	46376-46381	VBN	denotes	taken
T10742	46382-46387	NN	denotes	place
T10743	46387-46389	,	denotes	,
T10744	46389-46393	WDT	denotes	that
T10746	46394-46400	MD	denotes	should
T10747	46401-46403	VB	denotes	be
T10748	46404-46408	RBR	denotes	more
T10749	46409-46417	RB	denotes	severely
T10745	46418-46426	VBN	denotes	affected
T10750	46426-46427	.	denotes	.
T10751	46427-46562	sentence	denotes	Since it is the proximal elements which are more severely affected, the explanation for the difference in severity must lie elsewhere.
T10752	46428-46433	IN	denotes	Since
T10754	46434-46436	PRP	denotes	it
T10753	46437-46439	VBZ	denotes	is
T10756	46440-46443	DT	denotes	the
T10758	46444-46452	JJ	denotes	proximal
T10757	46453-46461	NNS	denotes	elements
T10759	46462-46467	WDT	denotes	which
T10761	46468-46471	VBP	denotes	are
T10762	46472-46476	RBR	denotes	more
T10763	46477-46485	RB	denotes	severely
T10760	46486-46494	VBN	denotes	affected
T10764	46494-46496	,	denotes	,
T10765	46496-46499	DT	denotes	the
T10766	46500-46511	NN	denotes	explanation
T10767	46512-46515	IN	denotes	for
T10768	46516-46519	DT	denotes	the
T10769	46520-46530	NN	denotes	difference
T10770	46531-46533	IN	denotes	in
T10771	46534-46542	NN	denotes	severity
T10772	46543-46547	MD	denotes	must
T10755	46548-46551	VB	denotes	lie
T10773	46552-46561	RB	denotes	elsewhere
T10774	46561-46562	.	denotes	.
T10775	46562-46676	sentence	denotes	This is most likely explained by compensation in the distal limb bud by BMP2, BMP4, and BMP7 produced in the AER.
T10776	46563-46567	DT	denotes	This
T10778	46568-46570	VBZ	denotes	is
T10779	46571-46575	RBS	denotes	most
T10780	46576-46582	RB	denotes	likely
T10777	46583-46592	VBN	denotes	explained
T10781	46593-46595	IN	denotes	by
T10782	46596-46608	NN	denotes	compensation
T10783	46609-46611	IN	denotes	in
T10784	46612-46615	DT	denotes	the
T10786	46616-46622	JJ	denotes	distal
T10787	46623-46627	NN	denotes	limb
T10785	46628-46631	NN	denotes	bud
T10788	46632-46634	IN	denotes	by
T10789	46635-46639	NN	denotes	BMP2
T10791	46639-46641	,	denotes	,
T10790	46641-46645	NN	denotes	BMP4
T10792	46645-46647	,	denotes	,
T10793	46647-46650	CC	denotes	and
T10794	46651-46655	NN	denotes	BMP7
T10795	46656-46664	VBN	denotes	produced
T10796	46665-46667	IN	denotes	in
T10797	46668-46671	DT	denotes	the
T10798	46672-46675	NN	denotes	AER
T10799	46675-46676	.	denotes	.
T10800	46676-46913	sentence	denotes	Compensation by BMPs in the AER also likely explains a difference seen between the Bmp4C/C; Prx1::cre mice in our experiments and a recently published analysis [16], which used the identical Bmp4 conditional allele and Prx1::cre driver.
T10801	46677-46689	NN	denotes	Compensation
T10803	46690-46692	IN	denotes	by
T10804	46693-46697	NNS	denotes	BMPs
T10805	46698-46700	IN	denotes	in
T10806	46701-46704	DT	denotes	the
T10807	46705-46708	NN	denotes	AER
T10808	46709-46713	RB	denotes	also
T10809	46714-46720	RB	denotes	likely
T10802	46721-46729	VBZ	denotes	explains
T10810	46730-46731	DT	denotes	a
T10811	46732-46742	NN	denotes	difference
T10812	46743-46747	VBN	denotes	seen
T10813	46748-46755	IN	denotes	between
T10814	46756-46759	DT	denotes	the
T10816	46760-46765	NN	denotes	Bmp4C
T10818	46765-46766	HYPH	denotes	/
T10817	46766-46767	NN	denotes	C
T10819	46767-46768	:	denotes	;
T10820	46769-46773	NN	denotes	Prx1
T10822	46773-46774	HYPH	denotes	:
T10823	46774-46775	HYPH	denotes	:
T10821	46775-46778	NN	denotes	cre
T10815	46779-46783	NNS	denotes	mice
T10824	46784-46786	IN	denotes	in
T10825	46787-46790	PRP$	denotes	our
T10826	46791-46802	NNS	denotes	experiments
T10827	46803-46806	CC	denotes	and
T10828	46807-46808	DT	denotes	a
T10830	46809-46817	RB	denotes	recently
T10831	46818-46827	VBN	denotes	published
T10829	46828-46836	NN	denotes	analysis
T10832	46837-46838	-LRB-	denotes	[
T10833	46838-46840	CD	denotes	16
T10834	46840-46841	-RRB-	denotes	]
T10835	46841-46843	,	denotes	,
T10836	46843-46848	WDT	denotes	which
T10837	46849-46853	VBD	denotes	used
T10838	46854-46857	DT	denotes	the
T10840	46858-46867	JJ	denotes	identical
T10841	46868-46872	NN	denotes	Bmp4
T10842	46873-46884	JJ	denotes	conditional
T10839	46885-46891	NN	denotes	allele
T10843	46892-46895	CC	denotes	and
T10844	46896-46900	NN	denotes	Prx1
T10846	46900-46901	HYPH	denotes	:
T10847	46901-46902	HYPH	denotes	:
T10845	46902-46905	NN	denotes	cre
T10848	46906-46912	NN	denotes	driver
T10849	46912-46913	.	denotes	.
T10850	46913-46990	sentence	denotes	The earlier report, like ours, described preaxial and postaxial polydactyly.
T10851	46914-46917	DT	denotes	The
T10853	46918-46925	JJR	denotes	earlier
T10852	46926-46932	NN	denotes	report
T10855	46932-46934	,	denotes	,
T10856	46934-46938	IN	denotes	like
T10857	46939-46943	PRP	denotes	ours
T10858	46943-46945	,	denotes	,
T10854	46945-46954	VBN	denotes	described
T10859	46955-46963	JJ	denotes	preaxial
T10861	46964-46967	CC	denotes	and
T10862	46968-46977	JJ	denotes	postaxial
T10860	46978-46989	NN	denotes	polydactyly
T10863	46989-46990	.	denotes	.
T10864	46990-47187	sentence	denotes	However, they observed these phenotypes with less variability and, moreover, described multiple preaxial and postaxial ectopic digits, while we saw, at most, a single preaxial and postaxial digit.
T10865	46991-46998	RB	denotes	However
T10867	46998-47000	,	denotes	,
T10868	47000-47004	PRP	denotes	they
T10866	47005-47013	VBD	denotes	observed
T10869	47014-47019	DT	denotes	these
T10870	47020-47030	NNS	denotes	phenotypes
T10871	47031-47035	IN	denotes	with
T10872	47036-47040	JJR	denotes	less
T10873	47041-47052	NN	denotes	variability
T10874	47053-47056	CC	denotes	and
T10875	47056-47058	,	denotes	,
T10876	47058-47066	RB	denotes	moreover
T10878	47066-47068	,	denotes	,
T10877	47068-47077	VBN	denotes	described
T10879	47078-47086	JJ	denotes	multiple
T10881	47087-47095	JJ	denotes	preaxial
T10882	47096-47099	CC	denotes	and
T10883	47100-47109	JJ	denotes	postaxial
T10884	47110-47117	JJ	denotes	ectopic
T10880	47118-47124	NNS	denotes	digits
T10885	47124-47126	,	denotes	,
T10886	47126-47131	IN	denotes	while
T10888	47132-47134	PRP	denotes	we
T10887	47135-47138	VBD	denotes	saw
T10889	47138-47140	,	denotes	,
T10890	47140-47142	RB	denotes	at
T10891	47143-47147	RBS	denotes	most
T10892	47147-47149	,	denotes	,
T10893	47149-47150	DT	denotes	a
T10895	47151-47157	JJ	denotes	single
T10896	47158-47166	JJ	denotes	preaxial
T10897	47167-47170	CC	denotes	and
T10898	47171-47180	JJ	denotes	postaxial
T10894	47181-47186	NN	denotes	digit
T10899	47186-47187	.	denotes	.
T10900	47187-47339	sentence	denotes	The difference is that the mice analyzed in the previous report had one null allele and one conditional allele, while ours had two conditional alleles.
T10901	47188-47191	DT	denotes	The
T10902	47192-47202	NN	denotes	difference
T10903	47203-47205	VBZ	denotes	is
T10904	47206-47210	IN	denotes	that
T10906	47211-47214	DT	denotes	the
T10907	47215-47219	NNS	denotes	mice
T10908	47220-47228	VBN	denotes	analyzed
T10909	47229-47231	IN	denotes	in
T10910	47232-47235	DT	denotes	the
T10912	47236-47244	JJ	denotes	previous
T10911	47245-47251	NN	denotes	report
T10905	47252-47255	VBD	denotes	had
T10913	47256-47259	CD	denotes	one
T10915	47260-47264	JJ	denotes	null
T10914	47265-47271	NN	denotes	allele
T10916	47272-47275	CC	denotes	and
T10917	47276-47279	CD	denotes	one
T10919	47280-47291	JJ	denotes	conditional
T10918	47292-47298	NN	denotes	allele
T10920	47298-47300	,	denotes	,
T10921	47300-47305	IN	denotes	while
T10923	47306-47310	PRP	denotes	ours
T10922	47311-47314	VBD	denotes	had
T10924	47315-47318	CD	denotes	two
T10926	47319-47330	JJ	denotes	conditional
T10925	47331-47338	NNS	denotes	alleles
T10927	47338-47339	.	denotes	.
T10928	47339-47542	sentence	denotes	Their mice, therefore, lost a half-dose of BMP4 from the AER in addition to complete loss in the mesenchyme, while our conditional alleles were only recombined in the mesenchyme by the Prx1::cre driver.
T10929	47340-47345	PRP$	denotes	Their
T10930	47346-47350	NNS	denotes	mice
T10932	47350-47352	,	denotes	,
T10933	47352-47361	RB	denotes	therefore
T10934	47361-47363	,	denotes	,
T10931	47363-47367	VBD	denotes	lost
T10935	47368-47369	DT	denotes	a
T10937	47370-47374	JJ	denotes	half
T10938	47374-47375	HYPH	denotes	-
T10936	47375-47379	NN	denotes	dose
T10939	47380-47382	IN	denotes	of
T10940	47383-47387	NN	denotes	BMP4
T10941	47388-47392	IN	denotes	from
T10942	47393-47396	DT	denotes	the
T10943	47397-47400	NN	denotes	AER
T10944	47401-47403	IN	denotes	in
T10945	47404-47412	NN	denotes	addition
T10946	47413-47415	IN	denotes	to
T10947	47416-47424	JJ	denotes	complete
T10948	47425-47429	NN	denotes	loss
T10949	47430-47432	IN	denotes	in
T10950	47433-47436	DT	denotes	the
T10951	47437-47447	NN	denotes	mesenchyme
T10952	47447-47449	,	denotes	,
T10953	47449-47454	IN	denotes	while
T10955	47455-47458	PRP$	denotes	our
T10957	47459-47470	JJ	denotes	conditional
T10956	47471-47478	NNS	denotes	alleles
T10958	47479-47483	VBD	denotes	were
T10959	47484-47488	RB	denotes	only
T10954	47489-47499	VBN	denotes	recombined
T10960	47500-47502	IN	denotes	in
T10961	47503-47506	DT	denotes	the
T10962	47507-47517	NN	denotes	mesenchyme
T10963	47518-47520	IN	denotes	by
T10964	47521-47524	DT	denotes	the
T10966	47525-47529	NN	denotes	Prx1
T10968	47529-47530	HYPH	denotes	:
T10969	47530-47531	HYPH	denotes	:
T10967	47531-47534	NN	denotes	cre
T10965	47535-47541	NN	denotes	driver
T10970	47541-47542	.	denotes	.
T10971	47542-47841	sentence	denotes	It is also possible that the difference in severity relates to the time required to recombine one as opposed to two floxed alleles; however, we do not favor this explanation as all detectable BMP4 expression is lost prior to any morphological differences between the mutant and wild-type limb buds.
T10972	47543-47545	PRP	denotes	It
T10973	47546-47548	VBZ	denotes	is
T10975	47549-47553	RB	denotes	also
T10976	47554-47562	JJ	denotes	possible
T10977	47563-47567	IN	denotes	that
T10979	47568-47571	DT	denotes	the
T10980	47572-47582	NN	denotes	difference
T10981	47583-47585	IN	denotes	in
T10982	47586-47594	NN	denotes	severity
T10978	47595-47602	VBZ	denotes	relates
T10983	47603-47605	IN	denotes	to
T10984	47606-47609	DT	denotes	the
T10985	47610-47614	NN	denotes	time
T10986	47615-47623	VBN	denotes	required
T10987	47624-47626	TO	denotes	to
T10988	47627-47636	VB	denotes	recombine
T10989	47637-47640	CD	denotes	one
T10990	47641-47643	IN	denotes	as
T10991	47644-47651	VBN	denotes	opposed
T10992	47652-47654	IN	denotes	to
T10993	47655-47658	CD	denotes	two
T10995	47659-47665	VBN	denotes	floxed
T10994	47666-47673	NNS	denotes	alleles
T10996	47673-47674	:	denotes	;
T10997	47675-47682	RB	denotes	however
T10998	47682-47684	,	denotes	,
T10999	47684-47686	PRP	denotes	we
T11000	47687-47689	VBP	denotes	do
T11001	47690-47693	RB	denotes	not
T10974	47694-47699	VB	denotes	favor
T11002	47700-47704	DT	denotes	this
T11003	47705-47716	NN	denotes	explanation
T11004	47717-47719	IN	denotes	as
T11006	47720-47723	DT	denotes	all
T11008	47724-47734	JJ	denotes	detectable
T11009	47735-47739	NN	denotes	BMP4
T11007	47740-47750	NN	denotes	expression
T11010	47751-47753	VBZ	denotes	is
T11005	47754-47758	VBN	denotes	lost
T11011	47759-47764	JJ	denotes	prior
T11012	47765-47767	IN	denotes	to
T11013	47768-47771	DT	denotes	any
T11015	47772-47785	JJ	denotes	morphological
T11014	47786-47797	NNS	denotes	differences
T11016	47798-47805	IN	denotes	between
T11017	47806-47809	DT	denotes	the
T11019	47810-47816	JJ	denotes	mutant
T11020	47817-47820	CC	denotes	and
T11021	47821-47825	JJ	denotes	wild
T11023	47825-47826	HYPH	denotes	-
T11022	47826-47830	NN	denotes	type
T11024	47831-47835	NN	denotes	limb
T11018	47836-47840	NNS	denotes	buds
T11025	47840-47841	.	denotes	.
T11026	47841-47954	sentence	denotes	Finally, differences in genetic background could contribute to the differences as the Prx1::cre line is outbred.
T11027	47842-47849	RB	denotes	Finally
T11029	47849-47851	,	denotes	,
T11030	47851-47862	NNS	denotes	differences
T11031	47863-47865	IN	denotes	in
T11032	47866-47873	JJ	denotes	genetic
T11033	47874-47884	NN	denotes	background
T11034	47885-47890	MD	denotes	could
T11028	47891-47901	VB	denotes	contribute
T11035	47902-47904	IN	denotes	to
T11036	47905-47908	DT	denotes	the
T11037	47909-47920	NNS	denotes	differences
T11038	47921-47923	IN	denotes	as
T11040	47924-47927	DT	denotes	the
T11042	47928-47932	NN	denotes	Prx1
T11044	47932-47933	:	denotes	:
T11045	47933-47934	:	denotes	:
T11043	47934-47937	NN	denotes	cre
T11041	47938-47942	NN	denotes	line
T11046	47943-47945	VBZ	denotes	is
T11039	47946-47953	VBN	denotes	outbred
T11047	47953-47954	.	denotes	.
T11048	47954-48051	sentence	denotes	A final aspect of patterning previously associated with BMP signaling is interdigital apoptosis.
T11049	47955-47956	DT	denotes	A
T11051	47957-47962	JJ	denotes	final
T11050	47963-47969	NN	denotes	aspect
T11053	47970-47972	IN	denotes	of
T11054	47973-47983	NN	denotes	patterning
T11055	47984-47994	RB	denotes	previously
T11056	47995-48005	VBN	denotes	associated
T11057	48006-48010	IN	denotes	with
T11058	48011-48014	NN	denotes	BMP
T11059	48015-48024	NN	denotes	signaling
T11052	48025-48027	VBZ	denotes	is
T11060	48028-48040	JJ	denotes	interdigital
T11061	48041-48050	NN	denotes	apoptosis
T11062	48050-48051	.	denotes	.
T11063	48051-48125	sentence	denotes	Consistent with this, we see some syndactyly in the BMP2-deficient limbs.
T11064	48052-48062	JJ	denotes	Consistent
T11066	48063-48067	IN	denotes	with
T11067	48068-48072	DT	denotes	this
T11068	48072-48074	,	denotes	,
T11069	48074-48076	PRP	denotes	we
T11065	48077-48080	VBP	denotes	see
T11070	48081-48085	DT	denotes	some
T11071	48086-48096	NN	denotes	syndactyly
T11072	48097-48099	IN	denotes	in
T11073	48100-48103	DT	denotes	the
T11075	48104-48108	NN	denotes	BMP2
T11077	48108-48109	HYPH	denotes	-
T11076	48109-48118	JJ	denotes	deficient
T11074	48119-48124	NNS	denotes	limbs
T11078	48124-48125	.	denotes	.
T11079	48125-48399	sentence	denotes	The fact that this is variable, and limited to the 3/4 interdigit, implies that the threshold for induction of apoptosis is low enough that loss of BMP2, BMP4 or BMP7 or BMP2 and BMP7 still leaves sufficient BMP signaling for most interdigital cell death to occur normally.
T11080	48126-48129	DT	denotes	The
T11081	48130-48134	NN	denotes	fact
T11083	48135-48139	IN	denotes	that
T11085	48140-48144	DT	denotes	this
T11084	48145-48147	VBZ	denotes	is
T11086	48148-48156	JJ	denotes	variable
T11087	48156-48158	,	denotes	,
T11088	48158-48161	CC	denotes	and
T11089	48162-48169	VBN	denotes	limited
T11090	48170-48172	IN	denotes	to
T11091	48173-48176	DT	denotes	the
T11093	48177-48178	CD	denotes	3
T11095	48178-48179	HYPH	denotes	/
T11094	48179-48180	CD	denotes	4
T11092	48181-48191	NN	denotes	interdigit
T11096	48191-48193	,	denotes	,
T11082	48193-48200	VBZ	denotes	implies
T11097	48201-48205	IN	denotes	that
T11099	48206-48209	DT	denotes	the
T11100	48210-48219	NN	denotes	threshold
T11101	48220-48223	IN	denotes	for
T11102	48224-48233	NN	denotes	induction
T11103	48234-48236	IN	denotes	of
T11104	48237-48246	NN	denotes	apoptosis
T11098	48247-48249	VBZ	denotes	is
T11105	48250-48253	JJ	denotes	low
T11106	48254-48260	RB	denotes	enough
T11107	48261-48265	IN	denotes	that
T11109	48266-48270	NN	denotes	loss
T11110	48271-48273	IN	denotes	of
T11111	48274-48278	NN	denotes	BMP2
T11112	48278-48280	,	denotes	,
T11113	48280-48284	NN	denotes	BMP4
T11114	48285-48287	CC	denotes	or
T11115	48288-48292	NN	denotes	BMP7
T11116	48293-48295	CC	denotes	or
T11117	48296-48300	NN	denotes	BMP2
T11118	48301-48304	CC	denotes	and
T11119	48305-48309	NN	denotes	BMP7
T11120	48310-48315	RB	denotes	still
T11108	48316-48322	VBZ	denotes	leaves
T11121	48323-48333	JJ	denotes	sufficient
T11123	48334-48337	NN	denotes	BMP
T11122	48338-48347	NN	denotes	signaling
T11124	48348-48351	IN	denotes	for
T11126	48352-48356	JJS	denotes	most
T11128	48357-48369	JJ	denotes	interdigital
T11129	48370-48374	NN	denotes	cell
T11127	48375-48380	NN	denotes	death
T11130	48381-48383	TO	denotes	to
T11125	48384-48389	VB	denotes	occur
T11131	48390-48398	RB	denotes	normally
T11132	48398-48399	.	denotes	.
T11133	48399-48517	sentence	denotes	However, loss of both BMP2 and BMP4 results in complete soft tissue syndactyly, i.e., webbing between all the digits.
T11134	48400-48407	RB	denotes	However
T11136	48407-48409	,	denotes	,
T11137	48409-48413	NN	denotes	loss
T11138	48414-48416	IN	denotes	of
T11139	48417-48421	CC	denotes	both
T11140	48422-48426	NN	denotes	BMP2
T11141	48427-48430	CC	denotes	and
T11142	48431-48435	NN	denotes	BMP4
T11135	48436-48443	VBZ	denotes	results
T11143	48444-48446	IN	denotes	in
T11144	48447-48455	JJ	denotes	complete
T11146	48456-48460	JJ	denotes	soft
T11147	48461-48467	NN	denotes	tissue
T11145	48468-48478	NN	denotes	syndactyly
T11148	48478-48480	,	denotes	,
T11149	48480-48484	FW	denotes	i.e.
T11151	48484-48486	,	denotes	,
T11150	48486-48493	NN	denotes	webbing
T11152	48494-48501	IN	denotes	between
T11153	48502-48505	PDT	denotes	all
T11155	48506-48509	DT	denotes	the
T11154	48510-48516	NNS	denotes	digits
T11156	48516-48517	.	denotes	.
T11157	48517-48686	sentence	denotes	Although BMP activity has been previously implicated in regulating interdigital apoptosis, this is the first genetic verification of their requirement for this process.
T11158	48518-48526	IN	denotes	Although
T11160	48527-48530	NN	denotes	BMP
T11161	48531-48539	NN	denotes	activity
T11162	48540-48543	VBZ	denotes	has
T11163	48544-48548	VBN	denotes	been
T11164	48549-48559	RB	denotes	previously
T11159	48560-48570	VBN	denotes	implicated
T11166	48571-48573	IN	denotes	in
T11167	48574-48584	VBG	denotes	regulating
T11169	48585-48597	JJ	denotes	interdigital
T11168	48598-48607	NN	denotes	apoptosis
T11170	48607-48609	,	denotes	,
T11171	48609-48613	DT	denotes	this
T11165	48614-48616	VBZ	denotes	is
T11172	48617-48620	DT	denotes	the
T11174	48621-48626	JJ	denotes	first
T11175	48627-48634	JJ	denotes	genetic
T11173	48635-48647	NN	denotes	verification
T11176	48648-48650	IN	denotes	of
T11177	48651-48656	PRP$	denotes	their
T11178	48657-48668	NN	denotes	requirement
T11179	48669-48672	IN	denotes	for
T11180	48673-48677	DT	denotes	this
T11181	48678-48685	NN	denotes	process
T11182	48685-48686	.	denotes	.
T11683	48688-48691	NN	denotes	BMP
T11684	48692-48701	NN	denotes	Signaling
T11685	48702-48705	CC	denotes	and
T11686	48706-48714	JJ	denotes	Skeletal
T11687	48715-48726	NN	denotes	Development
T11688	48726-48897	sentence	denotes	As discussed above, our data indicate that threshold levels of BMP signaling are required for initiating chondrogenic condensation, consistent with prior results [26–28].
T11689	48727-48729	IN	denotes	As
T11690	48730-48739	VBN	denotes	discussed
T11692	48740-48745	RB	denotes	above
T11693	48745-48747	,	denotes	,
T11694	48747-48750	PRP$	denotes	our
T11695	48751-48755	NNS	denotes	data
T11691	48756-48764	VBP	denotes	indicate
T11696	48765-48769	IN	denotes	that
T11698	48770-48779	NN	denotes	threshold
T11699	48780-48786	NNS	denotes	levels
T11700	48787-48789	IN	denotes	of
T11701	48790-48793	NN	denotes	BMP
T11702	48794-48803	NN	denotes	signaling
T11703	48804-48807	VBP	denotes	are
T11697	48808-48816	VBN	denotes	required
T11704	48817-48820	IN	denotes	for
T11705	48821-48831	VBG	denotes	initiating
T11706	48832-48844	JJ	denotes	chondrogenic
T11707	48845-48857	NN	denotes	condensation
T11708	48857-48859	,	denotes	,
T11709	48859-48869	JJ	denotes	consistent
T11710	48870-48874	IN	denotes	with
T11711	48875-48880	JJ	denotes	prior
T11712	48881-48888	NNS	denotes	results
T11713	48889-48890	-LRB-	denotes	[
T11714	48890-48892	CD	denotes	26
T11715	48892-48893	SYM	denotes	–
T11716	48893-48895	CD	denotes	28
T11717	48895-48896	-RRB-	denotes	]
T11718	48896-48897	.	denotes	.
T11719	48897-49081	sentence	denotes	However, once chondrogenesis begins, cartilage differentiation can be sustained, albeit with some detectable delay, in the absence of BMP2 and BMP4 or in the absence of BMP2 and BMP7.
T11720	48898-48905	RB	denotes	However
T11722	48905-48907	,	denotes	,
T11723	48907-48911	IN	denotes	once
T11725	48912-48926	NN	denotes	chondrogenesis
T11724	48927-48933	VBZ	denotes	begins
T11726	48933-48935	,	denotes	,
T11727	48935-48944	NN	denotes	cartilage
T11728	48945-48960	NN	denotes	differentiation
T11729	48961-48964	MD	denotes	can
T11730	48965-48967	VB	denotes	be
T11721	48968-48977	VBN	denotes	sustained
T11731	48977-48979	,	denotes	,
T11732	48979-48985	IN	denotes	albeit
T11733	48986-48990	IN	denotes	with
T11734	48991-48995	DT	denotes	some
T11736	48996-49006	JJ	denotes	detectable
T11735	49007-49012	NN	denotes	delay
T11737	49012-49014	,	denotes	,
T11738	49014-49016	IN	denotes	in
T11739	49017-49020	DT	denotes	the
T11740	49021-49028	NN	denotes	absence
T11741	49029-49031	IN	denotes	of
T11742	49032-49036	NN	denotes	BMP2
T11743	49037-49040	CC	denotes	and
T11744	49041-49045	NN	denotes	BMP4
T11745	49046-49048	CC	denotes	or
T11746	49049-49051	IN	denotes	in
T11747	49052-49055	DT	denotes	the
T11748	49056-49063	NN	denotes	absence
T11749	49064-49066	IN	denotes	of
T11750	49067-49071	NN	denotes	BMP2
T11751	49072-49075	CC	denotes	and
T11752	49076-49080	NN	denotes	BMP7
T11753	49080-49081	.	denotes	.
T11754	49081-49349	sentence	denotes	Thus, the expression of either BMP5, BMP6, and BMP7 (in the absence of BMP2 and BMP4) or the expression of BMP4, BMP5, and BMP6 (in the absence of BMP2 and BMP7) is sufficient to support mesenchymal condensation and chondrocyte differentiation in the developing limb.
T11755	49082-49086	RB	denotes	Thus
T11757	49086-49088	,	denotes	,
T11758	49088-49091	DT	denotes	the
T11759	49092-49102	NN	denotes	expression
T11760	49103-49105	IN	denotes	of
T11761	49106-49112	CC	denotes	either
T11762	49113-49117	NN	denotes	BMP5
T11763	49117-49119	,	denotes	,
T11764	49119-49123	NN	denotes	BMP6
T11765	49123-49125	,	denotes	,
T11766	49125-49128	CC	denotes	and
T11767	49129-49133	NN	denotes	BMP7
T11768	49134-49135	-LRB-	denotes	(
T11769	49135-49137	IN	denotes	in
T11770	49138-49141	DT	denotes	the
T11771	49142-49149	NN	denotes	absence
T11772	49150-49152	IN	denotes	of
T11773	49153-49157	NN	denotes	BMP2
T11774	49158-49161	CC	denotes	and
T11775	49162-49166	NN	denotes	BMP4
T11776	49166-49167	-RRB-	denotes	)
T11777	49168-49170	CC	denotes	or
T11778	49171-49174	DT	denotes	the
T11779	49175-49185	NN	denotes	expression
T11780	49186-49188	IN	denotes	of
T11781	49189-49193	NN	denotes	BMP4
T11782	49193-49195	,	denotes	,
T11783	49195-49199	NN	denotes	BMP5
T11784	49199-49201	,	denotes	,
T11785	49201-49204	CC	denotes	and
T11786	49205-49209	NN	denotes	BMP6
T11787	49210-49211	-LRB-	denotes	(
T11788	49211-49213	IN	denotes	in
T11789	49214-49217	DT	denotes	the
T11790	49218-49225	NN	denotes	absence
T11791	49226-49228	IN	denotes	of
T11792	49229-49233	NN	denotes	BMP2
T11793	49234-49237	CC	denotes	and
T11794	49238-49242	NN	denotes	BMP7
T11795	49242-49243	-RRB-	denotes	)
T11756	49244-49246	VBZ	denotes	is
T11796	49247-49257	JJ	denotes	sufficient
T11797	49258-49260	TO	denotes	to
T11798	49261-49268	VB	denotes	support
T11799	49269-49280	JJ	denotes	mesenchymal
T11800	49281-49293	NN	denotes	condensation
T11801	49294-49297	CC	denotes	and
T11802	49298-49309	NN	denotes	chondrocyte
T11803	49310-49325	NN	denotes	differentiation
T11804	49326-49328	IN	denotes	in
T11805	49329-49332	DT	denotes	the
T11807	49333-49343	VBG	denotes	developing
T11806	49344-49348	NN	denotes	limb
T11808	49348-49349	.	denotes	.
T11809	49349-49698	sentence	denotes	In one set of previous studies implicating BMP signaling in chondrogenesis, Yoon et al. [26] showed that if mice lose both Alk3 (BmprIa) and Alk6 (BmpIb), two of the three type I receptors used in BMP signal transduction, there is a dramatic decrease in the size of skeletal primordial due to a reduction of proliferation and increase in apoptosis.
T11810	49350-49352	IN	denotes	In
T11812	49353-49356	CD	denotes	one
T11813	49357-49360	NN	denotes	set
T11814	49361-49363	IN	denotes	of
T11815	49364-49372	JJ	denotes	previous
T11816	49373-49380	NNS	denotes	studies
T11817	49381-49392	VBG	denotes	implicating
T11818	49393-49396	NN	denotes	BMP
T11819	49397-49406	NN	denotes	signaling
T11820	49407-49409	IN	denotes	in
T11821	49410-49424	NN	denotes	chondrogenesis
T11822	49424-49426	,	denotes	,
T11823	49426-49430	NNP	denotes	Yoon
T11824	49431-49433	FW	denotes	et
T11825	49434-49437	FW	denotes	al.
T11826	49438-49439	-LRB-	denotes	[
T11827	49439-49441	CD	denotes	26
T11828	49441-49442	-RRB-	denotes	]
T11811	49443-49449	VBD	denotes	showed
T11829	49450-49454	IN	denotes	that
T11831	49455-49457	IN	denotes	if
T11833	49458-49462	NNS	denotes	mice
T11832	49463-49467	VBP	denotes	lose
T11834	49468-49472	CC	denotes	both
T11835	49473-49477	NN	denotes	Alk3
T11836	49478-49479	-LRB-	denotes	(
T11837	49479-49485	NN	denotes	BmprIa
T11838	49485-49486	-RRB-	denotes	)
T11839	49487-49490	CC	denotes	and
T11840	49491-49495	NN	denotes	Alk6
T11841	49496-49497	-LRB-	denotes	(
T11842	49497-49502	NN	denotes	BmpIb
T11843	49502-49503	-RRB-	denotes	)
T11844	49503-49505	,	denotes	,
T11845	49505-49508	CD	denotes	two
T11846	49509-49511	IN	denotes	of
T11847	49512-49515	DT	denotes	the
T11849	49516-49521	CD	denotes	three
T11850	49522-49526	NN	denotes	type
T11851	49527-49528	CD	denotes	I
T11848	49529-49538	NNS	denotes	receptors
T11852	49539-49543	VBN	denotes	used
T11853	49544-49546	IN	denotes	in
T11854	49547-49550	NN	denotes	BMP
T11856	49551-49557	NN	denotes	signal
T11855	49558-49570	NN	denotes	transduction
T11857	49570-49572	,	denotes	,
T11858	49572-49577	EX	denotes	there
T11830	49578-49580	VBZ	denotes	is
T11859	49581-49582	DT	denotes	a
T11861	49583-49591	JJ	denotes	dramatic
T11860	49592-49600	NN	denotes	decrease
T11862	49601-49603	IN	denotes	in
T11863	49604-49607	DT	denotes	the
T11864	49608-49612	NN	denotes	size
T11865	49613-49615	IN	denotes	of
T11866	49616-49624	JJ	denotes	skeletal
T11867	49625-49635	NN	denotes	primordial
T11868	49636-49639	IN	denotes	due
T11869	49640-49642	IN	denotes	to
T11870	49643-49644	DT	denotes	a
T11871	49645-49654	NN	denotes	reduction
T11872	49655-49657	IN	denotes	of
T11873	49658-49671	NN	denotes	proliferation
T11874	49672-49675	CC	denotes	and
T11875	49676-49684	NN	denotes	increase
T11876	49685-49687	IN	denotes	in
T11877	49688-49697	NN	denotes	apoptosis
T11878	49697-49698	.	denotes	.
T11879	49698-49886	sentence	denotes	The size of the skeletal elements in Bmp2C/C; Bmp4C/C; Prx1::cre limbs are also decreased compared to those of control mice, although not to the extent seen in the double receptor mutant.
T11880	49699-49702	DT	denotes	The
T11881	49703-49707	NN	denotes	size
T11883	49708-49710	IN	denotes	of
T11884	49711-49714	DT	denotes	the
T11886	49715-49723	JJ	denotes	skeletal
T11885	49724-49732	NNS	denotes	elements
T11887	49733-49735	IN	denotes	in
T11888	49736-49741	NN	denotes	Bmp2C
T11890	49741-49742	HYPH	denotes	/
T11889	49742-49743	NN	denotes	C
T11892	49743-49744	:	denotes	;
T11893	49745-49750	NN	denotes	Bmp4C
T11895	49750-49751	HYPH	denotes	/
T11894	49751-49752	NN	denotes	C
T11896	49752-49753	:	denotes	;
T11897	49754-49758	NN	denotes	Prx1
T11899	49758-49759	HYPH	denotes	:
T11900	49759-49760	HYPH	denotes	:
T11898	49760-49763	NN	denotes	cre
T11891	49764-49769	NNS	denotes	limbs
T11901	49770-49773	VBP	denotes	are
T11902	49774-49778	RB	denotes	also
T11882	49779-49788	VBN	denotes	decreased
T11903	49789-49797	VBN	denotes	compared
T11904	49798-49800	IN	denotes	to
T11905	49801-49806	DT	denotes	those
T11906	49807-49809	IN	denotes	of
T11907	49810-49817	NN	denotes	control
T11908	49818-49822	NNS	denotes	mice
T11909	49822-49824	,	denotes	,
T11910	49824-49832	IN	denotes	although
T11912	49833-49836	RB	denotes	not
T11911	49837-49839	IN	denotes	to
T11913	49840-49843	DT	denotes	the
T11914	49844-49850	NN	denotes	extent
T11915	49851-49855	VBN	denotes	seen
T11916	49856-49858	IN	denotes	in
T11917	49859-49862	DT	denotes	the
T11919	49863-49869	JJ	denotes	double
T11920	49870-49878	NN	denotes	receptor
T11918	49879-49885	NN	denotes	mutant
T11921	49885-49886	.	denotes	.
T11922	49886-50080	sentence	denotes	We do not know if reduced size of the skeletal elements in our study is similarly due to reduced proliferation and increase in apoptosis, lack of replacement by bone, or a combination of these.
T11923	49887-49889	PRP	denotes	We
T11925	49890-49892	VBP	denotes	do
T11926	49893-49896	RB	denotes	not
T11924	49897-49901	VB	denotes	know
T11927	49902-49904	IN	denotes	if
T11929	49905-49912	VBN	denotes	reduced
T11930	49913-49917	NN	denotes	size
T11931	49918-49920	IN	denotes	of
T11932	49921-49924	DT	denotes	the
T11934	49925-49933	JJ	denotes	skeletal
T11933	49934-49942	NNS	denotes	elements
T11935	49943-49945	IN	denotes	in
T11936	49946-49949	PRP$	denotes	our
T11937	49950-49955	NN	denotes	study
T11928	49956-49958	VBZ	denotes	is
T11938	49959-49968	RB	denotes	similarly
T11939	49969-49972	IN	denotes	due
T11940	49973-49975	IN	denotes	to
T11941	49976-49983	VBN	denotes	reduced
T11942	49984-49997	NN	denotes	proliferation
T11943	49998-50001	CC	denotes	and
T11944	50002-50010	NN	denotes	increase
T11945	50011-50013	IN	denotes	in
T11946	50014-50023	NN	denotes	apoptosis
T11947	50023-50025	,	denotes	,
T11948	50025-50029	NN	denotes	lack
T11949	50030-50032	IN	denotes	of
T11950	50033-50044	NN	denotes	replacement
T11951	50045-50047	IN	denotes	by
T11952	50048-50052	NN	denotes	bone
T11953	50052-50054	,	denotes	,
T11954	50054-50056	CC	denotes	or
T11955	50057-50058	DT	denotes	a
T11956	50059-50070	NN	denotes	combination
T11957	50071-50073	IN	denotes	of
T11958	50074-50079	DT	denotes	these
T11959	50079-50080	.	denotes	.
T11960	50080-50283	sentence	denotes	It is possible that a triple mutant removing BMP2, BMP4, and BMP7 activities would have a similar phenotype as the BmprIa, BmprIb double mutant, although these could also be compensated from other BMPs.
T11961	50081-50083	PRP	denotes	It
T11962	50084-50086	VBZ	denotes	is
T11963	50087-50095	JJ	denotes	possible
T11964	50096-50100	IN	denotes	that
T11966	50101-50102	DT	denotes	a
T11968	50103-50109	JJ	denotes	triple
T11967	50110-50116	NN	denotes	mutant
T11969	50117-50125	VBG	denotes	removing
T11970	50126-50130	NN	denotes	BMP2
T11972	50130-50132	,	denotes	,
T11973	50132-50136	NN	denotes	BMP4
T11974	50136-50138	,	denotes	,
T11975	50138-50141	CC	denotes	and
T11976	50142-50146	NN	denotes	BMP7
T11971	50147-50157	NNS	denotes	activities
T11977	50158-50163	MD	denotes	would
T11965	50164-50168	VB	denotes	have
T11978	50169-50170	DT	denotes	a
T11980	50171-50178	JJ	denotes	similar
T11979	50179-50188	NN	denotes	phenotype
T11981	50189-50191	IN	denotes	as
T11982	50192-50195	DT	denotes	the
T11984	50196-50202	NN	denotes	BmprIa
T11986	50202-50204	,	denotes	,
T11985	50204-50210	NN	denotes	BmprIb
T11987	50211-50217	JJ	denotes	double
T11983	50218-50224	NN	denotes	mutant
T11988	50224-50226	,	denotes	,
T11989	50226-50234	IN	denotes	although
T11991	50235-50240	DT	denotes	these
T11992	50241-50246	MD	denotes	could
T11993	50247-50251	RB	denotes	also
T11994	50252-50254	VB	denotes	be
T11990	50255-50266	VBN	denotes	compensated
T11995	50267-50271	IN	denotes	from
T11996	50272-50277	JJ	denotes	other
T11997	50278-50282	NNS	denotes	BMPs
T11998	50282-50283	.	denotes	.
T11999	50283-50556	sentence	denotes	We do know, however, that osteogenesis is defective in these mice, although bone formation proceeds normally in siblings retaining one functional copy of either the Bmp2 or Bmp4 gene (Figure S4), as it does in mice completely deficient in BMP2 and BMP7 (Figures 1 and S2).
T12000	50284-50286	PRP	denotes	We
T12002	50287-50289	VBP	denotes	do
T12001	50290-50294	VB	denotes	know
T12003	50294-50296	,	denotes	,
T12004	50296-50303	RB	denotes	however
T12005	50303-50305	,	denotes	,
T12006	50305-50309	IN	denotes	that
T12008	50310-50322	NN	denotes	osteogenesis
T12007	50323-50325	VBZ	denotes	is
T12009	50326-50335	JJ	denotes	defective
T12010	50336-50338	IN	denotes	in
T12011	50339-50344	DT	denotes	these
T12012	50345-50349	NNS	denotes	mice
T12013	50349-50351	,	denotes	,
T12014	50351-50359	IN	denotes	although
T12016	50360-50364	NN	denotes	bone
T12017	50365-50374	NN	denotes	formation
T12015	50375-50383	VBZ	denotes	proceeds
T12018	50384-50392	RB	denotes	normally
T12019	50393-50395	IN	denotes	in
T12020	50396-50404	NNS	denotes	siblings
T12021	50405-50414	VBG	denotes	retaining
T12022	50415-50418	CD	denotes	one
T12024	50419-50429	JJ	denotes	functional
T12023	50430-50434	NN	denotes	copy
T12025	50435-50437	IN	denotes	of
T12026	50438-50444	CC	denotes	either
T12028	50445-50448	DT	denotes	the
T12029	50449-50453	NN	denotes	Bmp2
T12030	50454-50456	CC	denotes	or
T12031	50457-50461	NN	denotes	Bmp4
T12027	50462-50466	NN	denotes	gene
T12032	50467-50468	-LRB-	denotes	(
T12034	50468-50474	NN	denotes	Figure
T12033	50475-50477	NN	denotes	S4
T12035	50477-50478	-RRB-	denotes	)
T12036	50478-50480	,	denotes	,
T12037	50480-50482	IN	denotes	as
T12039	50483-50485	PRP	denotes	it
T12040	50486-50490	VBZ	denotes	does
T12041	50491-50493	IN	denotes	in
T12042	50494-50498	NNS	denotes	mice
T12043	50499-50509	RB	denotes	completely
T12044	50510-50519	JJ	denotes	deficient
T12045	50520-50522	IN	denotes	in
T12046	50523-50527	NN	denotes	BMP2
T12047	50528-50531	CC	denotes	and
T12048	50532-50536	NN	denotes	BMP7
T12049	50537-50538	-LRB-	denotes	(
T12050	50538-50545	NNS	denotes	Figures
T12051	50546-50547	CD	denotes	1
T12052	50548-50551	CC	denotes	and
T12038	50552-50554	NN	denotes	S2
T12053	50554-50555	-RRB-	denotes	)
T12054	50555-50556	.	denotes	.
T12055	50556-50759	sentence	denotes	The phenotype we observe in limbs of Bmp2C/C; Bmp4C/C; Prx1::cre mice is similar to the phenotype reported for osterix knockout mice [46], which exhibited severe defects in osteoblastic differentiation.
T12056	50557-50560	DT	denotes	The
T12057	50561-50570	NN	denotes	phenotype
T12059	50571-50573	PRP	denotes	we
T12060	50574-50581	VBP	denotes	observe
T12061	50582-50584	IN	denotes	in
T12062	50585-50590	NNS	denotes	limbs
T12063	50591-50593	IN	denotes	of
T12064	50594-50599	NN	denotes	Bmp2C
T12066	50599-50600	HYPH	denotes	/
T12065	50600-50601	NN	denotes	C
T12068	50601-50602	:	denotes	;
T12069	50603-50608	NN	denotes	Bmp4C
T12071	50608-50609	HYPH	denotes	/
T12070	50609-50610	NN	denotes	C
T12072	50610-50611	:	denotes	;
T12073	50612-50616	NN	denotes	Prx1
T12075	50616-50617	HYPH	denotes	:
T12076	50617-50618	HYPH	denotes	:
T12074	50618-50621	NN	denotes	cre
T12067	50622-50626	NNS	denotes	mice
T12058	50627-50629	VBZ	denotes	is
T12077	50630-50637	JJ	denotes	similar
T12078	50638-50640	IN	denotes	to
T12079	50641-50644	DT	denotes	the
T12080	50645-50654	NN	denotes	phenotype
T12081	50655-50663	VBN	denotes	reported
T12082	50664-50667	IN	denotes	for
T12083	50668-50675	NN	denotes	osterix
T12084	50676-50684	NN	denotes	knockout
T12085	50685-50689	NNS	denotes	mice
T12086	50690-50691	-LRB-	denotes	[
T12087	50691-50693	CD	denotes	46
T12088	50693-50694	-RRB-	denotes	]
T12089	50694-50696	,	denotes	,
T12090	50696-50701	WDT	denotes	which
T12091	50702-50711	VBD	denotes	exhibited
T12092	50712-50718	JJ	denotes	severe
T12093	50719-50726	NNS	denotes	defects
T12094	50727-50729	IN	denotes	in
T12095	50730-50742	JJ	denotes	osteoblastic
T12096	50743-50758	NN	denotes	differentiation
T12097	50758-50759	.	denotes	.
T12098	50759-51077	sentence	denotes	Since we observe fibroblastic cells adjacent to mineralized cartilage that have characteristics of osteoprogenitor cells but fail to express osterix in the absence of both BMP2 and BMP4, one important role of BMP2 and BMP4 during endochondral ossification may be to induce osterix gene expression in osteoprogenitors.
T12099	50760-50765	IN	denotes	Since
T12101	50766-50768	PRP	denotes	we
T12100	50769-50776	VBP	denotes	observe
T12103	50777-50789	JJ	denotes	fibroblastic
T12104	50790-50795	NNS	denotes	cells
T12105	50796-50804	JJ	denotes	adjacent
T12106	50805-50807	IN	denotes	to
T12107	50808-50819	VBN	denotes	mineralized
T12108	50820-50829	NN	denotes	cartilage
T12109	50830-50834	WDT	denotes	that
T12110	50835-50839	VBP	denotes	have
T12111	50840-50855	NNS	denotes	characteristics
T12112	50856-50858	IN	denotes	of
T12113	50859-50874	NN	denotes	osteoprogenitor
T12114	50875-50880	NNS	denotes	cells
T12115	50881-50884	CC	denotes	but
T12116	50885-50889	VBP	denotes	fail
T12117	50890-50892	TO	denotes	to
T12118	50893-50900	VB	denotes	express
T12119	50901-50908	NN	denotes	osterix
T12120	50909-50911	IN	denotes	in
T12121	50912-50915	DT	denotes	the
T12122	50916-50923	NN	denotes	absence
T12123	50924-50926	IN	denotes	of
T12124	50927-50931	CC	denotes	both
T12125	50932-50936	NN	denotes	BMP2
T12126	50937-50940	CC	denotes	and
T12127	50941-50945	NN	denotes	BMP4
T12128	50945-50947	,	denotes	,
T12129	50947-50950	CD	denotes	one
T12131	50951-50960	JJ	denotes	important
T12130	50961-50965	NN	denotes	role
T12132	50966-50968	IN	denotes	of
T12133	50969-50973	NN	denotes	BMP2
T12134	50974-50977	CC	denotes	and
T12135	50978-50982	NN	denotes	BMP4
T12136	50983-50989	IN	denotes	during
T12137	50990-51002	JJ	denotes	endochondral
T12138	51003-51015	NN	denotes	ossification
T12139	51016-51019	MD	denotes	may
T12102	51020-51022	VB	denotes	be
T12140	51023-51025	TO	denotes	to
T12141	51026-51032	VB	denotes	induce
T12142	51033-51040	NN	denotes	osterix
T12144	51041-51045	NN	denotes	gene
T12143	51046-51056	NN	denotes	expression
T12145	51057-51059	IN	denotes	in
T12146	51060-51076	NNS	denotes	osteoprogenitors
T12147	51076-51077	.	denotes	.
T12148	51077-51273	sentence	denotes	Strikingly, however, we do observe osterix expression in the bone collar of E16.5 mice missing functional copies of both the Bmp2 and Bmp4 genes (K. Tsuji and A. Bandyopadhyay, unpublished data).
T12149	51078-51088	RB	denotes	Strikingly
T12151	51088-51090	,	denotes	,
T12152	51090-51097	RB	denotes	however
T12153	51097-51099	,	denotes	,
T12154	51099-51101	PRP	denotes	we
T12155	51102-51104	VBP	denotes	do
T12150	51105-51112	VB	denotes	observe
T12156	51113-51120	NN	denotes	osterix
T12157	51121-51131	NN	denotes	expression
T12158	51132-51134	IN	denotes	in
T12159	51135-51138	DT	denotes	the
T12161	51139-51143	NN	denotes	bone
T12160	51144-51150	NN	denotes	collar
T12162	51151-51153	IN	denotes	of
T12163	51154-51159	NN	denotes	E16.5
T12164	51160-51164	NNS	denotes	mice
T12165	51165-51172	VBG	denotes	missing
T12166	51173-51183	JJ	denotes	functional
T12167	51184-51190	NNS	denotes	copies
T12168	51191-51193	IN	denotes	of
T12169	51194-51198	CC	denotes	both
T12171	51199-51202	DT	denotes	the
T12172	51203-51207	NN	denotes	Bmp2
T12173	51208-51211	CC	denotes	and
T12174	51212-51216	NN	denotes	Bmp4
T12170	51217-51222	NNS	denotes	genes
T12175	51223-51224	-LRB-	denotes	(
T12176	51224-51226	NNP	denotes	K.
T12177	51227-51232	NNP	denotes	Tsuji
T12178	51233-51236	CC	denotes	and
T12179	51237-51239	NNP	denotes	A.
T12180	51240-51253	NNP	denotes	Bandyopadhyay
T12181	51253-51255	,	denotes	,
T12182	51255-51266	JJ	denotes	unpublished
T12183	51267-51271	NNS	denotes	data
T12184	51271-51272	-RRB-	denotes	)
T12185	51272-51273	.	denotes	.
T12186	51273-51531	sentence	denotes	This suggests that varying levels of expression from different BMP genes during preaxial and postaxial development result in fluctuations in total levels of BMP signaling, which at distinct times are above or below the threshold for supporting osteogenesis.
T12187	51274-51278	DT	denotes	This
T12188	51279-51287	VBZ	denotes	suggests
T12189	51288-51292	IN	denotes	that
T12191	51293-51300	VBG	denotes	varying
T12192	51301-51307	NNS	denotes	levels
T12193	51308-51310	IN	denotes	of
T12194	51311-51321	NN	denotes	expression
T12195	51322-51326	IN	denotes	from
T12196	51327-51336	JJ	denotes	different
T12198	51337-51340	NN	denotes	BMP
T12197	51341-51346	NNS	denotes	genes
T12199	51347-51353	IN	denotes	during
T12200	51354-51362	JJ	denotes	preaxial
T12202	51363-51366	CC	denotes	and
T12203	51367-51376	JJ	denotes	postaxial
T12201	51377-51388	NN	denotes	development
T12190	51389-51395	VBP	denotes	result
T12204	51396-51398	IN	denotes	in
T12205	51399-51411	NNS	denotes	fluctuations
T12206	51412-51414	IN	denotes	in
T12207	51415-51420	JJ	denotes	total
T12208	51421-51427	NNS	denotes	levels
T12209	51428-51430	IN	denotes	of
T12210	51431-51434	NN	denotes	BMP
T12211	51435-51444	NN	denotes	signaling
T12212	51444-51446	,	denotes	,
T12213	51446-51451	WDT	denotes	which
T12215	51452-51454	IN	denotes	at
T12216	51455-51463	JJ	denotes	distinct
T12217	51464-51469	NNS	denotes	times
T12214	51470-51473	VBP	denotes	are
T12218	51474-51479	IN	denotes	above
T12219	51480-51482	CC	denotes	or
T12220	51483-51488	IN	denotes	below
T12221	51489-51492	DT	denotes	the
T12222	51493-51502	NN	denotes	threshold
T12223	51503-51506	IN	denotes	for
T12224	51507-51517	VBG	denotes	supporting
T12225	51518-51530	NN	denotes	osteogenesis
T12226	51530-51531	.	denotes	.
T12227	51531-51789	sentence	denotes	While many osteogenic BMP molecules, apart from BMP2 and BMP4, such as BMP5, BMP6, and BMP7, are expressed during the endochondral process, our data suggest that these BMP molecules cannot compensate for the combined loss of BMP2 and BMP4 in bone formation.
T12228	51532-51537	IN	denotes	While
T12230	51538-51542	JJ	denotes	many
T12232	51543-51553	JJ	denotes	osteogenic
T12233	51554-51557	NN	denotes	BMP
T12231	51558-51567	NNS	denotes	molecules
T12234	51567-51569	,	denotes	,
T12235	51569-51574	RB	denotes	apart
T12236	51575-51579	IN	denotes	from
T12237	51580-51584	NN	denotes	BMP2
T12238	51585-51588	CC	denotes	and
T12239	51589-51593	NN	denotes	BMP4
T12240	51593-51595	,	denotes	,
T12241	51595-51599	JJ	denotes	such
T12242	51600-51602	IN	denotes	as
T12243	51603-51607	NN	denotes	BMP5
T12244	51607-51609	,	denotes	,
T12245	51609-51613	NN	denotes	BMP6
T12246	51613-51615	,	denotes	,
T12247	51615-51618	CC	denotes	and
T12248	51619-51623	NN	denotes	BMP7
T12249	51623-51625	,	denotes	,
T12250	51625-51628	VBP	denotes	are
T12229	51629-51638	VBN	denotes	expressed
T12252	51639-51645	IN	denotes	during
T12253	51646-51649	DT	denotes	the
T12255	51650-51662	JJ	denotes	endochondral
T12254	51663-51670	NN	denotes	process
T12256	51670-51672	,	denotes	,
T12257	51672-51675	PRP$	denotes	our
T12258	51676-51680	NNS	denotes	data
T12251	51681-51688	VBP	denotes	suggest
T12259	51689-51693	IN	denotes	that
T12261	51694-51699	DT	denotes	these
T12263	51700-51703	NN	denotes	BMP
T12262	51704-51713	NNS	denotes	molecules
T12264	51714-51717	MD	denotes	can
T12265	51717-51720	RB	denotes	not
T12260	51721-51731	VB	denotes	compensate
T12266	51732-51735	IN	denotes	for
T12267	51736-51739	DT	denotes	the
T12269	51740-51748	VBN	denotes	combined
T12268	51749-51753	NN	denotes	loss
T12270	51754-51756	IN	denotes	of
T12271	51757-51761	NN	denotes	BMP2
T12272	51762-51765	CC	denotes	and
T12273	51766-51770	NN	denotes	BMP4
T12274	51771-51773	IN	denotes	in
T12275	51774-51778	NN	denotes	bone
T12276	51779-51788	NN	denotes	formation
T12277	51788-51789	.	denotes	.
T12278	51789-51894	sentence	denotes	Recent biochemical and genetic experiments have suggested that individual BMPs have identical functions.
T12279	51790-51796	JJ	denotes	Recent
T12281	51797-51808	JJ	denotes	biochemical
T12282	51809-51812	CC	denotes	and
T12283	51813-51820	JJ	denotes	genetic
T12280	51821-51832	NNS	denotes	experiments
T12285	51833-51837	VBP	denotes	have
T12284	51838-51847	VBN	denotes	suggested
T12286	51848-51852	IN	denotes	that
T12288	51853-51863	JJ	denotes	individual
T12289	51864-51868	NNS	denotes	BMPs
T12287	51869-51873	VBP	denotes	have
T12290	51874-51883	JJ	denotes	identical
T12291	51884-51893	NNS	denotes	functions
T12292	51893-51894	.	denotes	.
T12293	51894-52037	sentence	denotes	BMP2, BMP4, BMP5, BMP6, and BMP7 can utilize the same type I (Alk2, Alk3, and Alk6) and type II receptors (BMP RII, ActRII, and ActRIIb) [47].
T12294	51895-51899	NN	denotes	BMP2
T12296	51899-51901	,	denotes	,
T12297	51901-51905	NN	denotes	BMP4
T12298	51905-51907	,	denotes	,
T12299	51907-51911	NN	denotes	BMP5
T12300	51911-51913	,	denotes	,
T12301	51913-51917	NN	denotes	BMP6
T12302	51917-51919	,	denotes	,
T12303	51919-51922	CC	denotes	and
T12304	51923-51927	NN	denotes	BMP7
T12305	51928-51931	MD	denotes	can
T12295	51932-51939	VB	denotes	utilize
T12306	51940-51943	DT	denotes	the
T12308	51944-51948	JJ	denotes	same
T12309	51949-51953	NN	denotes	type
T12310	51954-51955	CD	denotes	I
T12311	51956-51957	-LRB-	denotes	(
T12312	51957-51961	NN	denotes	Alk2
T12313	51961-51963	,	denotes	,
T12314	51963-51967	NN	denotes	Alk3
T12315	51967-51969	,	denotes	,
T12316	51969-51972	CC	denotes	and
T12317	51973-51977	NN	denotes	Alk6
T12318	51977-51978	-RRB-	denotes	)
T12319	51979-51982	CC	denotes	and
T12320	51983-51987	NN	denotes	type
T12321	51988-51990	CD	denotes	II
T12307	51991-52000	NNS	denotes	receptors
T12322	52001-52002	-LRB-	denotes	(
T12324	52002-52005	NN	denotes	BMP
T12323	52006-52009	NN	denotes	RII
T12325	52009-52011	,	denotes	,
T12326	52011-52017	NN	denotes	ActRII
T12327	52017-52019	,	denotes	,
T12328	52019-52022	CC	denotes	and
T12329	52023-52030	NN	denotes	ActRIIb
T12330	52030-52031	-RRB-	denotes	)
T12331	52032-52033	-LRB-	denotes	[
T12332	52033-52035	CD	denotes	47
T12333	52035-52036	-RRB-	denotes	]
T12334	52036-52037	.	denotes	.
T12335	52037-52323	sentence	denotes	Once the complex between ligand and receptors is formed, BMP2, BMP4, BMP5, BMP6, and BMP7 direct the phosphorylation of the same set of BMP receptor–specific Smads (1, 5, or 8), and the signal that is transduced by each BMP appears to be identical in the skeletal target cells [48–51].
T12336	52038-52042	IN	denotes	Once
T12338	52043-52046	DT	denotes	the
T12339	52047-52054	NN	denotes	complex
T12340	52055-52062	IN	denotes	between
T12341	52063-52069	NN	denotes	ligand
T12342	52070-52073	CC	denotes	and
T12343	52074-52083	NNS	denotes	receptors
T12344	52084-52086	VBZ	denotes	is
T12337	52087-52093	VBN	denotes	formed
T12346	52093-52095	,	denotes	,
T12347	52095-52099	NN	denotes	BMP2
T12348	52099-52101	,	denotes	,
T12349	52101-52105	NN	denotes	BMP4
T12350	52105-52107	,	denotes	,
T12351	52107-52111	NN	denotes	BMP5
T12352	52111-52113	,	denotes	,
T12353	52113-52117	NN	denotes	BMP6
T12354	52117-52119	,	denotes	,
T12355	52119-52122	CC	denotes	and
T12356	52123-52127	NN	denotes	BMP7
T12345	52128-52134	VBP	denotes	direct
T12357	52135-52138	DT	denotes	the
T12358	52139-52154	NN	denotes	phosphorylation
T12359	52155-52157	IN	denotes	of
T12360	52158-52161	DT	denotes	the
T12362	52162-52166	JJ	denotes	same
T12361	52167-52170	NN	denotes	set
T12363	52171-52173	IN	denotes	of
T12364	52174-52177	NN	denotes	BMP
T12366	52178-52186	NN	denotes	receptor
T12367	52186-52187	HYPH	denotes	–
T12368	52187-52195	JJ	denotes	specific
T12365	52196-52201	NNS	denotes	Smads
T12369	52202-52203	-LRB-	denotes	(
T12370	52203-52204	CD	denotes	1
T12371	52204-52206	,	denotes	,
T12372	52206-52207	CD	denotes	5
T12373	52207-52209	,	denotes	,
T12374	52209-52211	CC	denotes	or
T12375	52212-52213	CD	denotes	8
T12376	52213-52214	-RRB-	denotes	)
T12377	52214-52216	,	denotes	,
T12378	52216-52219	CC	denotes	and
T12379	52220-52223	DT	denotes	the
T12380	52224-52230	NN	denotes	signal
T12382	52231-52235	WDT	denotes	that
T12384	52236-52238	VBZ	denotes	is
T12383	52239-52249	VBN	denotes	transduced
T12385	52250-52252	IN	denotes	by
T12386	52253-52257	DT	denotes	each
T12387	52258-52261	NN	denotes	BMP
T12381	52262-52269	VBZ	denotes	appears
T12388	52270-52272	TO	denotes	to
T12389	52273-52275	VB	denotes	be
T12390	52276-52285	JJ	denotes	identical
T12391	52286-52288	IN	denotes	in
T12392	52289-52292	DT	denotes	the
T12394	52293-52301	JJ	denotes	skeletal
T12395	52302-52308	NN	denotes	target
T12393	52309-52314	NNS	denotes	cells
T12396	52315-52316	-LRB-	denotes	[
T12397	52316-52318	CD	denotes	48
T12398	52318-52319	SYM	denotes	–
T12399	52319-52321	CD	denotes	51
T12400	52321-52322	-RRB-	denotes	]
T12401	52322-52323	.	denotes	.
T12402	52323-52536	sentence	denotes	BMPs have also been shown to activate MAPK and AKT pathways and, based on current published information, each of the osteogenic BMPs appears to have the same capacity to activate these signaling pathways [52–54].
T12403	52324-52328	NNS	denotes	BMPs
T12405	52329-52333	VBP	denotes	have
T12406	52334-52338	RB	denotes	also
T12407	52339-52343	VBN	denotes	been
T12404	52344-52349	VBN	denotes	shown
T12408	52350-52352	TO	denotes	to
T12409	52353-52361	VB	denotes	activate
T12410	52362-52366	NN	denotes	MAPK
T12412	52367-52370	CC	denotes	and
T12413	52371-52374	NN	denotes	AKT
T12411	52375-52383	NNS	denotes	pathways
T12414	52384-52387	CC	denotes	and
T12415	52387-52389	,	denotes	,
T12416	52389-52394	VBN	denotes	based
T12418	52395-52397	IN	denotes	on
T12419	52398-52405	JJ	denotes	current
T12421	52406-52415	VBN	denotes	published
T12420	52416-52427	NN	denotes	information
T12422	52427-52429	,	denotes	,
T12423	52429-52433	DT	denotes	each
T12424	52434-52436	IN	denotes	of
T12425	52437-52440	DT	denotes	the
T12427	52441-52451	JJ	denotes	osteogenic
T12426	52452-52456	NNS	denotes	BMPs
T12417	52457-52464	VBZ	denotes	appears
T12428	52465-52467	TO	denotes	to
T12429	52468-52472	VB	denotes	have
T12430	52473-52476	DT	denotes	the
T12432	52477-52481	JJ	denotes	same
T12431	52482-52490	NN	denotes	capacity
T12433	52491-52493	TO	denotes	to
T12434	52494-52502	VB	denotes	activate
T12435	52503-52508	DT	denotes	these
T12437	52509-52518	NN	denotes	signaling
T12436	52519-52527	NNS	denotes	pathways
T12438	52528-52529	-LRB-	denotes	[
T12439	52529-52531	CD	denotes	52
T12440	52531-52532	SYM	denotes	–
T12441	52532-52534	CD	denotes	54
T12442	52534-52535	-RRB-	denotes	]
T12443	52535-52536	.	denotes	.
T12444	52536-52929	sentence	denotes	When this information is considered along with our observation that one allele of either Bmp2 or Bmp4 can rescue the osteoblast differentiation phenotype observed in Bmp2C/C; Bmp4C/C; Prx1::cre mice (see Figures 1I, 1J, 1Q, and 1R and S4), we favor the hypothesis that bone formation requires a threshold amount of BMP signaling which is not met when both BMP2 and BMP4 are completely absent.
T12445	52537-52541	WRB	denotes	When
T12447	52542-52546	DT	denotes	this
T12448	52547-52558	NN	denotes	information
T12449	52559-52561	VBZ	denotes	is
T12446	52562-52572	VBN	denotes	considered
T12451	52573-52578	IN	denotes	along
T12452	52579-52583	IN	denotes	with
T12453	52584-52587	PRP$	denotes	our
T12454	52588-52599	NN	denotes	observation
T12455	52600-52604	IN	denotes	that
T12457	52605-52608	CD	denotes	one
T12458	52609-52615	NN	denotes	allele
T12459	52616-52618	IN	denotes	of
T12460	52619-52625	CC	denotes	either
T12461	52626-52630	NN	denotes	Bmp2
T12462	52631-52633	CC	denotes	or
T12463	52634-52638	NN	denotes	Bmp4
T12464	52639-52642	MD	denotes	can
T12456	52643-52649	VB	denotes	rescue
T12465	52650-52653	DT	denotes	the
T12467	52654-52664	NN	denotes	osteoblast
T12468	52665-52680	NN	denotes	differentiation
T12466	52681-52690	NN	denotes	phenotype
T12469	52691-52699	VBN	denotes	observed
T12470	52700-52702	IN	denotes	in
T12471	52703-52708	NN	denotes	Bmp2C
T12473	52708-52709	HYPH	denotes	/
T12472	52709-52710	NN	denotes	C
T12475	52710-52711	:	denotes	;
T12476	52712-52717	NN	denotes	Bmp4C
T12478	52717-52718	HYPH	denotes	/
T12477	52718-52719	NN	denotes	C
T12479	52719-52720	:	denotes	;
T12480	52721-52725	NN	denotes	Prx1
T12482	52725-52726	HYPH	denotes	:
T12483	52726-52727	HYPH	denotes	:
T12481	52727-52730	NN	denotes	cre
T12474	52731-52735	NNS	denotes	mice
T12484	52736-52737	-LRB-	denotes	(
T12485	52737-52740	VB	denotes	see
T12486	52741-52748	NNS	denotes	Figures
T12487	52749-52751	NN	denotes	1I
T12488	52751-52753	,	denotes	,
T12489	52753-52755	NN	denotes	1J
T12490	52755-52757	,	denotes	,
T12491	52757-52759	NN	denotes	1Q
T12492	52759-52761	,	denotes	,
T12493	52761-52764	CC	denotes	and
T12494	52765-52767	NN	denotes	1R
T12495	52768-52771	CC	denotes	and
T12496	52772-52774	NN	denotes	S4
T12497	52774-52775	-RRB-	denotes	)
T12498	52775-52777	,	denotes	,
T12499	52777-52779	PRP	denotes	we
T12450	52780-52785	VBP	denotes	favor
T12500	52786-52789	DT	denotes	the
T12501	52790-52800	NN	denotes	hypothesis
T12502	52801-52805	IN	denotes	that
T12504	52806-52810	NN	denotes	bone
T12505	52811-52820	NN	denotes	formation
T12503	52821-52829	VBZ	denotes	requires
T12506	52830-52831	DT	denotes	a
T12508	52832-52841	NN	denotes	threshold
T12507	52842-52848	NN	denotes	amount
T12509	52849-52851	IN	denotes	of
T12510	52852-52855	NN	denotes	BMP
T12511	52856-52865	NN	denotes	signaling
T12512	52866-52871	WDT	denotes	which
T12514	52872-52874	VBZ	denotes	is
T12515	52875-52878	RB	denotes	not
T12513	52879-52882	VBN	denotes	met
T12516	52883-52887	WRB	denotes	when
T12518	52888-52892	CC	denotes	both
T12519	52893-52897	NN	denotes	BMP2
T12520	52898-52901	CC	denotes	and
T12521	52902-52906	NN	denotes	BMP4
T12517	52907-52910	VBP	denotes	are
T12522	52911-52921	RB	denotes	completely
T12523	52922-52928	JJ	denotes	absent
T12524	52928-52929	.	denotes	.
T12525	52929-53039	sentence	denotes	We cannot, however, exclude the possibility that BMP2 and BMP4 have distinct functions in osteoblastogenesis.
T12526	52930-52932	PRP	denotes	We
T12528	52933-52936	MD	denotes	can
T12529	52936-52939	RB	denotes	not
T12530	52939-52941	,	denotes	,
T12531	52941-52948	RB	denotes	however
T12532	52948-52950	,	denotes	,
T12527	52950-52957	VB	denotes	exclude
T12533	52958-52961	DT	denotes	the
T12534	52962-52973	NN	denotes	possibility
T12535	52974-52978	IN	denotes	that
T12537	52979-52983	NN	denotes	BMP2
T12538	52984-52987	CC	denotes	and
T12539	52988-52992	NN	denotes	BMP4
T12536	52993-52997	VBP	denotes	have
T12540	52998-53006	JJ	denotes	distinct
T12541	53007-53016	NNS	denotes	functions
T12542	53017-53019	IN	denotes	in
T12543	53020-53038	NN	denotes	osteoblastogenesis
T12544	53038-53039	.	denotes	.
T12545	53039-53180	sentence	denotes	Our analysis of skeletogenesis indicates that BMP2 and BMP4 are prerequisite for osteoblastogenesis while less important for chondrogenesis.
T12546	53040-53043	PRP$	denotes	Our
T12547	53044-53052	NN	denotes	analysis
T12549	53053-53055	IN	denotes	of
T12550	53056-53070	NN	denotes	skeletogenesis
T12548	53071-53080	VBZ	denotes	indicates
T12551	53081-53085	IN	denotes	that
T12553	53086-53090	NN	denotes	BMP2
T12554	53091-53094	CC	denotes	and
T12555	53095-53099	NN	denotes	BMP4
T12552	53100-53103	VBP	denotes	are
T12556	53104-53116	JJ	denotes	prerequisite
T12557	53117-53120	IN	denotes	for
T12558	53121-53139	NN	denotes	osteoblastogenesis
T12559	53140-53145	IN	denotes	while
T12561	53146-53150	RBR	denotes	less
T12560	53151-53160	JJ	denotes	important
T12562	53161-53164	IN	denotes	for
T12563	53165-53179	NN	denotes	chondrogenesis
T12564	53179-53180	.	denotes	.
T12565	53180-53351	sentence	denotes	These studies provide the first evidence linking BMP2 and BMP4 with bone formation in an in vivo setting where the preceding events of chondrogenesis are not compromised.
T12566	53181-53186	DT	denotes	These
T12567	53187-53194	NNS	denotes	studies
T12568	53195-53202	VBP	denotes	provide
T12569	53203-53206	DT	denotes	the
T12571	53207-53212	JJ	denotes	first
T12570	53213-53221	NN	denotes	evidence
T12572	53222-53229	VBG	denotes	linking
T12573	53230-53234	NN	denotes	BMP2
T12574	53235-53238	CC	denotes	and
T12575	53239-53243	NN	denotes	BMP4
T12576	53244-53248	IN	denotes	with
T12577	53249-53253	NN	denotes	bone
T12578	53254-53263	NN	denotes	formation
T12579	53264-53266	IN	denotes	in
T12580	53267-53269	DT	denotes	an
T12582	53270-53272	FW	denotes	in
T12583	53273-53277	FW	denotes	vivo
T12581	53278-53285	VBG	denotes	setting
T12584	53286-53291	WRB	denotes	where
T12586	53292-53295	DT	denotes	the
T12588	53296-53305	VBG	denotes	preceding
T12587	53306-53312	NNS	denotes	events
T12589	53313-53315	IN	denotes	of
T12590	53316-53330	NN	denotes	chondrogenesis
T12585	53331-53334	VBP	denotes	are
T12591	53335-53338	RB	denotes	not
T12592	53339-53350	JJ	denotes	compromised
T12593	53350-53351	.	denotes	.
T12632	53376-53384	NNS	denotes	Reagents
T12633	53384-53385	.	denotes	.
T12634	53385-53491	sentence	denotes	Xylene, formamide, and acetic anhydride were purchased from Fisher Scientific (http://www.fishersci.com).
T12635	53386-53392	NN	denotes	Xylene
T12637	53392-53394	,	denotes	,
T12638	53394-53403	NN	denotes	formamide
T12639	53403-53405	,	denotes	,
T12640	53405-53408	CC	denotes	and
T12641	53409-53415	JJ	denotes	acetic
T12642	53416-53425	NN	denotes	anhydride
T12643	53426-53430	VBD	denotes	were
T12636	53431-53440	VBN	denotes	purchased
T12644	53441-53445	IN	denotes	from
T12645	53446-53452	NNP	denotes	Fisher
T12646	53453-53463	NNP	denotes	Scientific
T12647	53464-53465	-LRB-	denotes	(
T12648	53465-53489	NN	denotes	http://www.fishersci.com
T12649	53489-53490	-RRB-	denotes	)
T12650	53490-53491	.	denotes	.
T12651	53491-53668	sentence	denotes	Alizarin red S, toluidine blue, paraformaldehyde, polyvinylpyrrolidone, proteinase K, RNase A, tRNA, and dithiothreitol were purchased from Sigma (http://www.sigmaaldrich.com).
T12652	53492-53500	NN	denotes	Alizarin
T12654	53501-53504	JJ	denotes	red
T12653	53505-53506	NN	denotes	S
T12656	53506-53508	,	denotes	,
T12657	53508-53517	NN	denotes	toluidine
T12658	53518-53522	JJ	denotes	blue
T12659	53522-53524	,	denotes	,
T12660	53524-53540	NN	denotes	paraformaldehyde
T12661	53540-53542	,	denotes	,
T12662	53542-53562	NN	denotes	polyvinylpyrrolidone
T12663	53562-53564	,	denotes	,
T12664	53564-53574	NN	denotes	proteinase
T12665	53575-53576	NN	denotes	K
T12666	53576-53578	,	denotes	,
T12667	53578-53583	NN	denotes	RNase
T12668	53584-53585	NN	denotes	A
T12669	53585-53587	,	denotes	,
T12670	53587-53591	NN	denotes	tRNA
T12671	53591-53593	,	denotes	,
T12672	53593-53596	CC	denotes	and
T12673	53597-53611	NN	denotes	dithiothreitol
T12674	53612-53616	VBD	denotes	were
T12655	53617-53626	VBN	denotes	purchased
T12675	53627-53631	IN	denotes	from
T12676	53632-53637	NNP	denotes	Sigma
T12677	53638-53639	-LRB-	denotes	(
T12678	53639-53666	NN	denotes	http://www.sigmaaldrich.com
T12679	53666-53667	-RRB-	denotes	)
T12680	53667-53668	.	denotes	.
T12681	53668-53759	sentence	denotes	Alcian blue 8GX was purchased from Electron Microscopy Science (http://www.emsdiasum.com).
T12682	53669-53675	JJ	denotes	Alcian
T12684	53676-53680	JJ	denotes	blue
T12683	53681-53684	NN	denotes	8GX
T12686	53685-53688	VBD	denotes	was
T12685	53689-53698	VBN	denotes	purchased
T12687	53699-53703	IN	denotes	from
T12688	53704-53712	NNP	denotes	Electron
T12690	53713-53723	NNP	denotes	Microscopy
T12689	53724-53731	NNP	denotes	Science
T12691	53732-53733	-LRB-	denotes	(
T12692	53733-53757	NN	denotes	http://www.emsdiasum.com
T12693	53757-53758	-RRB-	denotes	)
T12694	53758-53759	.	denotes	.
T12695	53759-53832	sentence	denotes	Dextran sulfate was purchased from Pharmacia (http://www.pharmacia.com).
T12696	53760-53767	NN	denotes	Dextran
T12697	53768-53775	NN	denotes	sulfate
T12699	53776-53779	VBD	denotes	was
T12698	53780-53789	VBN	denotes	purchased
T12700	53790-53794	IN	denotes	from
T12701	53795-53804	NNP	denotes	Pharmacia
T12702	53805-53806	-LRB-	denotes	(
T12703	53806-53830	NN	denotes	http://www.pharmacia.com
T12704	53830-53831	-RRB-	denotes	)
T12705	53831-53832	.	denotes	.
T12757	53834-53844	NN	denotes	Generation
T12758	53845-53847	IN	denotes	of
T12759	53848-53852	NN	denotes	Bmp2
T12761	53853-53856	CC	denotes	and
T12762	53857-53861	NN	denotes	Bmp4
T12763	53862-53873	JJ	denotes	conditional
T12760	53874-53878	JJ	denotes	null
T12764	53879-53885	NN	denotes	allele
T12765	53885-53886	.	denotes	.
T12766	53886-54026	sentence	denotes	Mice carrying floxed Bmp2 allele were available through a material transfer agreement between Harvard University and Wyeth Pharmaceuticals.
T12767	53887-53891	NNS	denotes	Mice
T12769	53892-53900	VBG	denotes	carrying
T12770	53901-53907	VBN	denotes	floxed
T12772	53908-53912	NN	denotes	Bmp2
T12771	53913-53919	NN	denotes	allele
T12768	53920-53924	VBD	denotes	were
T12773	53925-53934	JJ	denotes	available
T12774	53935-53942	IN	denotes	through
T12775	53943-53944	DT	denotes	a
T12777	53945-53953	NN	denotes	material
T12778	53954-53962	NN	denotes	transfer
T12776	53963-53972	NN	denotes	agreement
T12779	53973-53980	IN	denotes	between
T12780	53981-53988	NNP	denotes	Harvard
T12781	53989-53999	NNP	denotes	University
T12782	54000-54003	CC	denotes	and
T12783	54004-54009	NNP	denotes	Wyeth
T12784	54010-54025	NNP	denotes	Pharmaceuticals
T12785	54025-54026	.	denotes	.
T12786	54026-54139	sentence	denotes	In these mice, LoxP sites were integrated to excise the entire protein-coding region in exon 3 of the Bmp2 gene.
T12787	54027-54029	IN	denotes	In
T12789	54030-54035	DT	denotes	these
T12790	54036-54040	NNS	denotes	mice
T12791	54040-54042	,	denotes	,
T12792	54042-54046	NN	denotes	LoxP
T12793	54047-54052	NNS	denotes	sites
T12794	54053-54057	VBD	denotes	were
T12788	54058-54068	VBN	denotes	integrated
T12795	54069-54071	TO	denotes	to
T12796	54072-54078	VB	denotes	excise
T12797	54079-54082	DT	denotes	the
T12799	54083-54089	JJ	denotes	entire
T12800	54090-54097	NN	denotes	protein
T12802	54097-54098	HYPH	denotes	-
T12801	54098-54104	VBG	denotes	coding
T12798	54105-54111	NN	denotes	region
T12803	54112-54114	IN	denotes	in
T12804	54115-54119	NN	denotes	exon
T12805	54120-54121	CD	denotes	3
T12806	54122-54124	IN	denotes	of
T12807	54125-54128	DT	denotes	the
T12809	54129-54133	NN	denotes	Bmp2
T12808	54134-54138	NN	denotes	gene
T12810	54138-54139	.	denotes	.
T12811	54139-54205	sentence	denotes	Generation of Bmp4 conditional allele has been described [16,55].
T12812	54140-54150	NN	denotes	Generation
T12814	54151-54153	IN	denotes	of
T12815	54154-54158	NN	denotes	Bmp4
T12817	54159-54170	JJ	denotes	conditional
T12816	54171-54177	NN	denotes	allele
T12818	54178-54181	VBZ	denotes	has
T12819	54182-54186	VBN	denotes	been
T12813	54187-54196	VBN	denotes	described
T12820	54197-54198	-LRB-	denotes	[
T12822	54198-54200	CD	denotes	16
T12823	54200-54201	,	denotes	,
T12821	54201-54203	CD	denotes	55
T12824	54203-54204	-RRB-	denotes	]
T12825	54204-54205	.	denotes	.
T12826	54205-54254	sentence	denotes	The Prx1::cre transgene has been described [32].
T12827	54206-54209	DT	denotes	The
T12829	54210-54214	NN	denotes	Prx1
T12831	54214-54215	HYPH	denotes	:
T12832	54215-54216	HYPH	denotes	:
T12830	54216-54219	NN	denotes	cre
T12828	54220-54229	NN	denotes	transgene
T12834	54230-54233	VBZ	denotes	has
T12835	54234-54238	VBN	denotes	been
T12833	54239-54248	VBN	denotes	described
T12836	54249-54250	-LRB-	denotes	[
T12837	54250-54252	CD	denotes	32
T12838	54252-54253	-RRB-	denotes	]
T12839	54253-54254	.	denotes	.
T13183	54256-54266	NN	denotes	Generation
T13184	54267-54269	IN	denotes	of
T13185	54270-54274	NN	denotes	Bmp2
T13187	54274-54276	,	denotes	,
T13186	54276-54280	NN	denotes	Bmp4
T13189	54281-54287	JJ	denotes	double
T13188	54288-54299	JJ	denotes	conditional
T13190	54300-54304	NNS	denotes	mice
T13191	54305-54308	CC	denotes	and
T13192	54309-54313	NN	denotes	Bmp2
T13193	54314-54325	JJ	denotes	conditional
T13195	54325-54327	,	denotes	,
T13196	54327-54331	NN	denotes	Bmp7
T13197	54332-54338	NN	denotes	mutant
T13194	54339-54343	NNS	denotes	mice
T13198	54343-54344	.	denotes	.
T13199	54344-54436	sentence	denotes	Bmp2 C/C animals were crossed with Bmp4 C/C animals to generate Bmp2 C/+; Bmp4 C/+ animals.
T13200	54345-54351	NN	denotes	Bmp2 C
T13202	54351-54352	HYPH	denotes	/
T13201	54352-54353	NN	denotes	C
T13203	54354-54361	NNS	denotes	animals
T13205	54362-54366	VBD	denotes	were
T13204	54367-54374	VBN	denotes	crossed
T13206	54375-54379	IN	denotes	with
T13207	54380-54386	NN	denotes	Bmp4 C
T13209	54386-54387	HYPH	denotes	/
T13208	54387-54388	NN	denotes	C
T13210	54389-54396	NNS	denotes	animals
T13211	54397-54399	TO	denotes	to
T13212	54400-54408	VB	denotes	generate
T13213	54409-54415	NN	denotes	Bmp2 C
T13215	54415-54416	HYPH	denotes	/
T13216	54416-54417	SYM	denotes	+
T13217	54417-54418	:	denotes	;
T13218	54419-54425	NN	denotes	Bmp4 C
T13219	54425-54426	HYPH	denotes	/
T13220	54426-54427	SYM	denotes	+
T13214	54428-54435	NNS	denotes	animals
T13221	54435-54436	.	denotes	.
T13222	54436-54573	sentence	denotes	These animals were crossed with Bmp2 C/C or Bmp4 C/C animals to generate Bmp2 C/C; Bmp4 C/+ or Bmp2 C/+; Bmp4 C/C animals, respectively.
T13223	54437-54442	DT	denotes	These
T13224	54443-54450	NNS	denotes	animals
T13226	54451-54455	VBD	denotes	were
T13225	54456-54463	VBN	denotes	crossed
T13227	54464-54468	IN	denotes	with
T13228	54469-54475	NN	denotes	Bmp2 C
T13230	54475-54476	HYPH	denotes	/
T13229	54476-54477	NN	denotes	C
T13232	54478-54480	CC	denotes	or
T13233	54481-54487	NN	denotes	Bmp4 C
T13235	54487-54488	HYPH	denotes	/
T13234	54488-54489	NN	denotes	C
T13231	54490-54497	NNS	denotes	animals
T13236	54498-54500	TO	denotes	to
T13237	54501-54509	VB	denotes	generate
T13238	54510-54516	NN	denotes	Bmp2 C
T13240	54516-54517	HYPH	denotes	/
T13239	54517-54518	NN	denotes	C
T13242	54518-54519	:	denotes	;
T13243	54520-54526	NN	denotes	Bmp4 C
T13244	54526-54527	HYPH	denotes	/
T13245	54527-54528	SYM	denotes	+
T13246	54529-54531	CC	denotes	or
T13247	54532-54538	NN	denotes	Bmp2 C
T13248	54538-54539	HYPH	denotes	/
T13249	54539-54540	SYM	denotes	+
T13250	54540-54541	:	denotes	;
T13251	54542-54548	NN	denotes	Bmp4 C
T13253	54548-54549	HYPH	denotes	/
T13252	54549-54550	NN	denotes	C
T13241	54551-54558	NNS	denotes	animals
T13254	54558-54560	,	denotes	,
T13255	54560-54572	RB	denotes	respectively
T13256	54572-54573	.	denotes	.
T13257	54573-54691	sentence	denotes	Bmp2 C/C; Bmp4 C/+ or Bmp2 C/+; Bmp4 C/C animals were crossed with each other to generate Bmp2 C/C; Bmp4 C/C animals.
T13258	54574-54580	NN	denotes	Bmp2 C
T13260	54580-54581	HYPH	denotes	/
T13259	54581-54582	NN	denotes	C
T13262	54582-54583	:	denotes	;
T13263	54584-54590	NN	denotes	Bmp4 C
T13264	54590-54591	HYPH	denotes	/
T13265	54591-54592	SYM	denotes	+
T13266	54593-54595	CC	denotes	or
T13267	54596-54602	NN	denotes	Bmp2 C
T13268	54602-54603	HYPH	denotes	/
T13269	54603-54604	SYM	denotes	+
T13270	54604-54605	:	denotes	;
T13271	54606-54612	NN	denotes	Bmp4 C
T13273	54612-54613	HYPH	denotes	/
T13272	54613-54614	NN	denotes	C
T13261	54615-54622	NNS	denotes	animals
T13275	54623-54627	VBD	denotes	were
T13274	54628-54635	VBN	denotes	crossed
T13276	54636-54640	IN	denotes	with
T13277	54641-54645	DT	denotes	each
T13278	54646-54651	JJ	denotes	other
T13279	54652-54654	TO	denotes	to
T13280	54655-54663	VB	denotes	generate
T13281	54664-54670	NN	denotes	Bmp2 C
T13283	54670-54671	HYPH	denotes	/
T13282	54671-54672	NN	denotes	C
T13285	54672-54673	:	denotes	;
T13286	54674-54680	NN	denotes	Bmp4 C
T13288	54680-54681	HYPH	denotes	/
T13287	54681-54682	NN	denotes	C
T13284	54683-54690	NNS	denotes	animals
T13289	54690-54691	.	denotes	.
T13290	54691-54819	sentence	denotes	Bmp2 C/C; Bmp4 C/C females were crossed with males bearing Prx1::cre transgene to generate Bmp2C/+; Bmp4C/+; Prx1::cre animals.
T13291	54692-54698	NN	denotes	Bmp2 C
T13293	54698-54699	HYPH	denotes	/
T13292	54699-54700	NN	denotes	C
T13295	54700-54701	:	denotes	;
T13296	54702-54708	NN	denotes	Bmp4 C
T13298	54708-54709	HYPH	denotes	/
T13297	54709-54710	NN	denotes	C
T13294	54711-54718	NNS	denotes	females
T13300	54719-54723	VBD	denotes	were
T13299	54724-54731	VBN	denotes	crossed
T13301	54732-54736	IN	denotes	with
T13302	54737-54742	NNS	denotes	males
T13303	54743-54750	VBG	denotes	bearing
T13304	54751-54755	NN	denotes	Prx1
T13306	54755-54756	HYPH	denotes	:
T13307	54756-54757	HYPH	denotes	:
T13305	54757-54760	NN	denotes	cre
T13308	54761-54770	NN	denotes	transgene
T13309	54771-54773	TO	denotes	to
T13310	54774-54782	VB	denotes	generate
T13311	54783-54788	NN	denotes	Bmp2C
T13313	54788-54789	HYPH	denotes	/
T13314	54789-54790	SYM	denotes	+
T13315	54790-54791	:	denotes	;
T13316	54792-54797	NN	denotes	Bmp4C
T13317	54797-54798	HYPH	denotes	/
T13318	54798-54799	SYM	denotes	+
T13319	54799-54800	:	denotes	;
T13320	54801-54805	NN	denotes	Prx1
T13322	54805-54806	HYPH	denotes	:
T13323	54806-54807	HYPH	denotes	:
T13321	54807-54810	NN	denotes	cre
T13312	54811-54818	NNS	denotes	animals
T13324	54818-54819	.	denotes	.
T13325	54819-54945	sentence	denotes	Bmp2C/+; Bmp4C/+; Prx1::cre males were crossed with Bmp2C/C; Bmp4C/C females to generate Bmp2C/C; Bmp4C/C; Prx1::cre animals.
T13326	54820-54825	NN	denotes	Bmp2C
T13328	54825-54826	HYPH	denotes	/
T13329	54826-54827	SYM	denotes	+
T13330	54827-54828	:	denotes	;
T13331	54829-54834	NN	denotes	Bmp4C
T13332	54834-54835	HYPH	denotes	/
T13333	54835-54836	SYM	denotes	+
T13334	54836-54837	:	denotes	;
T13335	54838-54842	NN	denotes	Prx1
T13337	54842-54843	HYPH	denotes	:
T13338	54843-54844	HYPH	denotes	:
T13336	54844-54847	NN	denotes	cre
T13327	54848-54853	NNS	denotes	males
T13340	54854-54858	VBD	denotes	were
T13339	54859-54866	VBN	denotes	crossed
T13341	54867-54871	IN	denotes	with
T13342	54872-54877	NN	denotes	Bmp2C
T13344	54877-54878	HYPH	denotes	/
T13343	54878-54879	NN	denotes	C
T13346	54879-54880	:	denotes	;
T13347	54881-54886	NN	denotes	Bmp4C
T13349	54886-54887	HYPH	denotes	/
T13348	54887-54888	NN	denotes	C
T13345	54889-54896	NNS	denotes	females
T13350	54897-54899	TO	denotes	to
T13351	54900-54908	VB	denotes	generate
T13352	54909-54914	NN	denotes	Bmp2C
T13354	54914-54915	HYPH	denotes	/
T13353	54915-54916	NN	denotes	C
T13356	54916-54917	:	denotes	;
T13357	54918-54923	NN	denotes	Bmp4C
T13359	54923-54924	HYPH	denotes	/
T13358	54924-54925	NN	denotes	C
T13360	54925-54926	:	denotes	;
T13361	54927-54931	NN	denotes	Prx1
T13363	54931-54932	HYPH	denotes	:
T13364	54932-54933	HYPH	denotes	:
T13362	54933-54936	NN	denotes	cre
T13355	54937-54944	NNS	denotes	animals
T13365	54944-54945	.	denotes	.
T13366	54945-55046	sentence	denotes	Bmp2C/C; Bmp4C/C mice carrying floxed alleles of the Bmp genes without Prx1::cre are wild-type (WT).
T13367	54946-54951	NN	denotes	Bmp2C
T13369	54951-54952	HYPH	denotes	/
T13368	54952-54953	NN	denotes	C
T13371	54953-54954	:	denotes	;
T13372	54955-54960	NN	denotes	Bmp4C
T13374	54960-54961	HYPH	denotes	/
T13373	54961-54962	NN	denotes	C
T13370	54963-54967	NNS	denotes	mice
T13376	54968-54976	VBG	denotes	carrying
T13377	54977-54983	VBN	denotes	floxed
T13378	54984-54991	NNS	denotes	alleles
T13379	54992-54994	IN	denotes	of
T13380	54995-54998	DT	denotes	the
T13382	54999-55002	NN	denotes	Bmp
T13381	55003-55008	NNS	denotes	genes
T13383	55009-55016	IN	denotes	without
T13384	55017-55021	NN	denotes	Prx1
T13386	55021-55022	HYPH	denotes	:
T13387	55022-55023	HYPH	denotes	:
T13385	55023-55026	NN	denotes	cre
T13375	55027-55030	VBP	denotes	are
T13388	55031-55035	JJ	denotes	wild
T13390	55035-55036	HYPH	denotes	-
T13389	55036-55040	NN	denotes	type
T13391	55041-55042	-LRB-	denotes	(
T13392	55042-55044	NN	denotes	WT
T13393	55044-55045	-RRB-	denotes	)
T13394	55045-55046	.	denotes	.
T13395	55046-55366	sentence	denotes	Genotyping of animals were done by PCR analyses with the primers (5′–3′) GTGTGGTCCACCGCATCAC (AHP2–9) and GGCAGACATTGTATCTCTAGG (AHP2–35) for Bmp2 and AGACTCTTTAGTGAGCATTTTCAAC (No. 79 [order of primers tried]) and AGCCCAATTTCCACAACTTC (No. 80) for Bmp4 following extraction of genomic DNA from embryonic or adult tail.
T13396	55047-55057	NN	denotes	Genotyping
T13398	55058-55060	IN	denotes	of
T13399	55061-55068	NNS	denotes	animals
T13400	55069-55073	VBD	denotes	were
T13397	55074-55078	VBN	denotes	done
T13401	55079-55081	IN	denotes	by
T13402	55082-55085	NN	denotes	PCR
T13403	55086-55094	NNS	denotes	analyses
T13404	55095-55099	IN	denotes	with
T13405	55100-55103	DT	denotes	the
T13407	55104-55111	NNS	denotes	primers
T13408	55112-55113	-LRB-	denotes	(
T13409	55113-55114	CD	denotes	5
T13410	55114-55115	SYM	denotes	′
T13411	55115-55116	SYM	denotes	–
T13412	55116-55117	CD	denotes	3
T13413	55117-55118	SYM	denotes	′
T13414	55118-55119	-RRB-	denotes	)
T13406	55120-55139	NN	denotes	GTGTGGTCCACCGCATCAC
T13415	55140-55141	-LRB-	denotes	(
T13416	55141-55145	NN	denotes	AHP2
T13417	55145-55146	SYM	denotes	–
T13418	55146-55147	CD	denotes	9
T13419	55147-55148	-RRB-	denotes	)
T13420	55149-55152	CC	denotes	and
T13421	55153-55174	NN	denotes	GGCAGACATTGTATCTCTAGG
T13422	55175-55176	-LRB-	denotes	(
T13423	55176-55180	NN	denotes	AHP2
T13424	55180-55181	SYM	denotes	–
T13425	55181-55183	CD	denotes	35
T13426	55183-55184	-RRB-	denotes	)
T13427	55185-55188	IN	denotes	for
T13428	55189-55193	NN	denotes	Bmp2
T13429	55194-55197	CC	denotes	and
T13430	55198-55223	NN	denotes	AGACTCTTTAGTGAGCATTTTCAAC
T13431	55224-55225	-LRB-	denotes	(
T13433	55225-55228	NN	denotes	No.
T13434	55229-55231	CD	denotes	79
T13435	55232-55233	-LRB-	denotes	[
T13432	55233-55238	NN	denotes	order
T13436	55239-55241	IN	denotes	of
T13437	55242-55249	NNS	denotes	primers
T13438	55250-55255	VBN	denotes	tried
T13439	55255-55256	-RRB-	denotes	]
T13440	55256-55257	-RRB-	denotes	)
T13441	55258-55261	CC	denotes	and
T13442	55262-55282	NN	denotes	AGCCCAATTTCCACAACTTC
T13443	55283-55284	-LRB-	denotes	(
T13444	55284-55287	NN	denotes	No.
T13445	55288-55290	CD	denotes	80
T13446	55290-55291	-RRB-	denotes	)
T13447	55292-55295	IN	denotes	for
T13448	55296-55300	NN	denotes	Bmp4
T13449	55301-55310	VBG	denotes	following
T13450	55311-55321	NN	denotes	extraction
T13451	55322-55324	IN	denotes	of
T13452	55325-55332	JJ	denotes	genomic
T13453	55333-55336	NN	denotes	DNA
T13454	55337-55341	IN	denotes	from
T13455	55342-55351	JJ	denotes	embryonic
T13457	55352-55354	CC	denotes	or
T13458	55355-55360	JJ	denotes	adult
T13456	55361-55365	NN	denotes	tail
T13459	55365-55366	.	denotes	.
T13460	55366-55483	sentence	denotes	For Bmp2, the floxed allele amplifies as a 545-bp product, while the wild-type allele amplifies as a 474-bp product.
T13461	55367-55370	IN	denotes	For
T13463	55371-55375	NN	denotes	Bmp2
T13464	55375-55377	,	denotes	,
T13465	55377-55380	DT	denotes	the
T13467	55381-55387	VBN	denotes	floxed
T13466	55388-55394	NN	denotes	allele
T13462	55395-55404	VBZ	denotes	amplifies
T13468	55405-55407	IN	denotes	as
T13469	55408-55409	DT	denotes	a
T13471	55410-55413	CD	denotes	545
T13473	55413-55414	HYPH	denotes	-
T13472	55414-55416	NN	denotes	bp
T13470	55417-55424	NN	denotes	product
T13474	55424-55426	,	denotes	,
T13475	55426-55431	IN	denotes	while
T13477	55432-55435	DT	denotes	the
T13479	55436-55440	JJ	denotes	wild
T13481	55440-55441	HYPH	denotes	-
T13480	55441-55445	NN	denotes	type
T13478	55446-55452	NN	denotes	allele
T13476	55453-55462	VBZ	denotes	amplifies
T13482	55463-55465	IN	denotes	as
T13483	55466-55467	DT	denotes	a
T13485	55468-55471	CD	denotes	474
T13487	55471-55472	HYPH	denotes	-
T13486	55472-55474	NN	denotes	bp
T13484	55475-55482	NN	denotes	product
T13488	55482-55483	.	denotes	.
T13489	55483-55600	sentence	denotes	For Bmp4, the floxed allele amplifies as a 220-bp product, while the wild-type allele amplifies as a 180-bp product.
T13490	55484-55487	IN	denotes	For
T13492	55488-55492	NN	denotes	Bmp4
T13493	55492-55494	,	denotes	,
T13494	55494-55497	DT	denotes	the
T13496	55498-55504	VBN	denotes	floxed
T13495	55505-55511	NN	denotes	allele
T13491	55512-55521	VBZ	denotes	amplifies
T13497	55522-55524	IN	denotes	as
T13498	55525-55526	DT	denotes	a
T13500	55527-55530	CD	denotes	220
T13502	55530-55531	HYPH	denotes	-
T13501	55531-55533	NN	denotes	bp
T13499	55534-55541	NN	denotes	product
T13503	55541-55543	,	denotes	,
T13504	55543-55548	IN	denotes	while
T13506	55549-55552	DT	denotes	the
T13508	55553-55557	JJ	denotes	wild
T13510	55557-55558	HYPH	denotes	-
T13509	55558-55562	NN	denotes	type
T13507	55563-55569	NN	denotes	allele
T13505	55570-55579	VBZ	denotes	amplifies
T13511	55580-55582	IN	denotes	as
T13512	55583-55584	DT	denotes	a
T13514	55585-55588	CD	denotes	180
T13516	55588-55589	HYPH	denotes	-
T13515	55589-55591	NN	denotes	bp
T13513	55592-55599	NN	denotes	product
T13517	55599-55600	.	denotes	.
T13518	55600-55747	sentence	denotes	Protocols utilized for mouse experiments were approved by the Harvard University Institutional Animal Care and Use Committee (Cliff Tabin #02735).
T13519	55601-55610	NNS	denotes	Protocols
T13521	55611-55619	VBN	denotes	utilized
T13522	55620-55623	IN	denotes	for
T13523	55624-55629	NN	denotes	mouse
T13524	55630-55641	NNS	denotes	experiments
T13525	55642-55646	VBD	denotes	were
T13520	55647-55655	VBN	denotes	approved
T13526	55656-55658	IN	denotes	by
T13527	55659-55662	DT	denotes	the
T13529	55663-55670	NNP	denotes	Harvard
T13530	55671-55681	NNP	denotes	University
T13531	55682-55695	NNP	denotes	Institutional
T13532	55696-55702	NNP	denotes	Animal
T13533	55703-55707	NNP	denotes	Care
T13534	55708-55711	CC	denotes	and
T13535	55712-55715	NNP	denotes	Use
T13528	55716-55725	NNP	denotes	Committee
T13536	55726-55727	-LRB-	denotes	(
T13538	55727-55732	NNP	denotes	Cliff
T13537	55733-55738	NNP	denotes	Tabin
T13539	55739-55740	SYM	denotes	#
T13540	55740-55745	CD	denotes	02735
T13541	55745-55746	-RRB-	denotes	)
T13542	55746-55747	.	denotes	.
T13543	55747-55948	sentence	denotes	The strategy for generating a Bmp2 conditional null in the BMP7 −/− background is similar to the strategy used for generating the Bmp2, Bmp4 double conditional allele with three important differences.
T13544	55748-55751	DT	denotes	The
T13545	55752-55760	NN	denotes	strategy
T13547	55761-55764	IN	denotes	for
T13548	55765-55775	VBG	denotes	generating
T13549	55776-55777	DT	denotes	a
T13551	55778-55782	NN	denotes	Bmp2
T13552	55783-55794	JJ	denotes	conditional
T13550	55795-55799	NN	denotes	null
T13553	55800-55802	IN	denotes	in
T13554	55803-55806	DT	denotes	the
T13556	55807-55811	NN	denotes	BMP7
T13557	55812-55813	SYM	denotes	−
T13558	55813-55814	HYPH	denotes	/
T13559	55814-55815	SYM	denotes	−
T13555	55816-55826	NN	denotes	background
T13546	55827-55829	VBZ	denotes	is
T13560	55830-55837	JJ	denotes	similar
T13561	55838-55840	IN	denotes	to
T13562	55841-55844	DT	denotes	the
T13563	55845-55853	NN	denotes	strategy
T13564	55854-55858	VBN	denotes	used
T13565	55859-55862	IN	denotes	for
T13566	55863-55873	VBG	denotes	generating
T13567	55874-55877	DT	denotes	the
T13569	55878-55882	NN	denotes	Bmp2
T13571	55882-55884	,	denotes	,
T13570	55884-55888	NN	denotes	Bmp4
T13573	55889-55895	JJ	denotes	double
T13572	55896-55907	JJ	denotes	conditional
T13568	55908-55914	NN	denotes	allele
T13574	55915-55919	IN	denotes	with
T13575	55920-55925	CD	denotes	three
T13577	55926-55935	JJ	denotes	important
T13576	55936-55947	NNS	denotes	differences
T13578	55947-55948	.	denotes	.
T13579	55948-56067	sentence	denotes	First, the Bmp7 −/− (strain kindly donated by Dr. Liz Robertson [12]) is not a conditional allele but a null mutation.
T13580	55949-55954	RB	denotes	First
T13582	55954-55956	,	denotes	,
T13583	55956-55959	DT	denotes	the
T13584	55960-55964	NN	denotes	Bmp7
T13585	55965-55966	SYM	denotes	−
T13586	55966-55967	HYPH	denotes	/
T13587	55967-55968	SYM	denotes	−
T13588	55969-55970	-LRB-	denotes	(
T13589	55970-55976	NN	denotes	strain
T13590	55977-55983	RB	denotes	kindly
T13591	55984-55991	VBN	denotes	donated
T13592	55992-55994	IN	denotes	by
T13593	55995-55998	NNP	denotes	Dr.
T13595	55999-56002	NNP	denotes	Liz
T13594	56003-56012	NNP	denotes	Robertson
T13596	56013-56014	-LRB-	denotes	[
T13597	56014-56016	CD	denotes	12
T13598	56016-56017	-RRB-	denotes	]
T13599	56017-56018	-RRB-	denotes	)
T13581	56019-56021	VBZ	denotes	is
T13600	56022-56025	CC	denotes	not
T13602	56026-56027	DT	denotes	a
T13603	56028-56039	JJ	denotes	conditional
T13601	56040-56046	NN	denotes	allele
T13604	56047-56050	CC	denotes	but
T13605	56051-56052	DT	denotes	a
T13607	56053-56057	JJ	denotes	null
T13606	56058-56066	NN	denotes	mutation
T13608	56066-56067	.	denotes	.
T13609	56067-56208	sentence	denotes	Second, Bmp7 −/− mice do not survive past birth due to kidney failure [12] and hence cannot be maintained as homozygous for the null allele.
T13610	56068-56074	RB	denotes	Second
T13612	56074-56076	,	denotes	,
T13613	56076-56080	NN	denotes	Bmp7
T13615	56081-56082	SYM	denotes	−
T13616	56082-56083	HYPH	denotes	/
T13617	56083-56084	SYM	denotes	−
T13614	56085-56089	NNS	denotes	mice
T13618	56090-56092	VBP	denotes	do
T13619	56093-56096	RB	denotes	not
T13611	56097-56104	VB	denotes	survive
T13620	56105-56109	IN	denotes	past
T13621	56110-56115	NN	denotes	birth
T13622	56116-56119	IN	denotes	due
T13623	56120-56122	IN	denotes	to
T13624	56123-56129	NN	denotes	kidney
T13625	56130-56137	NN	denotes	failure
T13626	56138-56139	-LRB-	denotes	[
T13627	56139-56141	CD	denotes	12
T13628	56141-56142	-RRB-	denotes	]
T13629	56143-56146	CC	denotes	and
T13630	56147-56152	RB	denotes	hence
T13632	56153-56156	MD	denotes	can
T13633	56156-56159	RB	denotes	not
T13634	56160-56162	VB	denotes	be
T13631	56163-56173	VBN	denotes	maintained
T13635	56174-56176	IN	denotes	as
T13636	56177-56187	JJ	denotes	homozygous
T13637	56188-56191	IN	denotes	for
T13638	56192-56195	DT	denotes	the
T13640	56196-56200	JJ	denotes	null
T13639	56201-56207	NN	denotes	allele
T13641	56207-56208	.	denotes	.
T13642	56208-56272	sentence	denotes	Third, Bmp2 and Bmp7 are on the same chromosome (Chromosome 2).
T13643	56209-56214	RB	denotes	Third
T13645	56214-56216	,	denotes	,
T13646	56216-56220	NN	denotes	Bmp2
T13647	56221-56224	CC	denotes	and
T13648	56225-56229	NN	denotes	Bmp7
T13644	56230-56233	VBP	denotes	are
T13649	56234-56236	IN	denotes	on
T13650	56237-56240	DT	denotes	the
T13652	56241-56245	JJ	denotes	same
T13651	56246-56256	NN	denotes	chromosome
T13653	56257-56258	-LRB-	denotes	(
T13654	56258-56268	NN	denotes	Chromosome
T13655	56269-56270	CD	denotes	2
T13656	56270-56271	-RRB-	denotes	)
T13657	56271-56272	.	denotes	.
T13658	56272-56389	sentence	denotes	To obtain Bmp2C/C, Bmp7 −/−; Prx1::cre we crossed Bmp2C/C, Bmp7 −/+ females with Bmp2C/+, Bmp7 −/+; Prx1::cre males.
T13659	56273-56275	TO	denotes	To
T13660	56276-56282	VB	denotes	obtain
T13662	56283-56288	NN	denotes	Bmp2C
T13664	56288-56289	HYPH	denotes	/
T13663	56289-56290	NN	denotes	C
T13665	56290-56292	,	denotes	,
T13666	56292-56296	NN	denotes	Bmp7
T13667	56297-56298	SYM	denotes	−
T13668	56298-56299	HYPH	denotes	/
T13669	56299-56300	SYM	denotes	−
T13670	56300-56301	:	denotes	;
T13671	56302-56306	NN	denotes	Prx1
T13673	56306-56307	HYPH	denotes	:
T13674	56307-56308	HYPH	denotes	:
T13672	56308-56311	NN	denotes	cre
T13675	56312-56314	PRP	denotes	we
T13661	56315-56322	VBD	denotes	crossed
T13676	56323-56328	NN	denotes	Bmp2C
T13678	56328-56329	HYPH	denotes	/
T13677	56329-56330	NN	denotes	C
T13680	56330-56332	,	denotes	,
T13681	56332-56336	NN	denotes	Bmp7
T13682	56337-56338	SYM	denotes	−
T13683	56338-56339	HYPH	denotes	/
T13684	56339-56340	SYM	denotes	+
T13679	56341-56348	NNS	denotes	females
T13685	56349-56353	IN	denotes	with
T13686	56354-56359	NN	denotes	Bmp2C
T13688	56359-56360	HYPH	denotes	/
T13689	56360-56361	SYM	denotes	+
T13690	56361-56363	,	denotes	,
T13691	56363-56367	NN	denotes	Bmp7
T13692	56368-56369	SYM	denotes	−
T13693	56369-56370	HYPH	denotes	/
T13694	56370-56371	SYM	denotes	+
T13695	56371-56372	:	denotes	;
T13696	56373-56377	NN	denotes	Prx1
T13698	56377-56378	HYPH	denotes	:
T13699	56378-56379	HYPH	denotes	:
T13697	56379-56382	NN	denotes	cre
T13687	56383-56388	NNS	denotes	males
T13700	56388-56389	.	denotes	.
T13701	56389-56480	sentence	denotes	Bmp2C/C, Bmp7 −/−; Prx1::cre progeny were obtained at a rate slightly higher than 1 in 16.
T13702	56390-56395	NN	denotes	Bmp2C
T13704	56395-56396	HYPH	denotes	/
T13703	56396-56397	NN	denotes	C
T13706	56397-56399	,	denotes	,
T13707	56399-56403	NN	denotes	Bmp7
T13708	56404-56405	SYM	denotes	−
T13709	56405-56406	HYPH	denotes	/
T13710	56406-56407	SYM	denotes	−
T13711	56407-56408	:	denotes	;
T13712	56409-56413	NN	denotes	Prx1
T13714	56413-56414	HYPH	denotes	:
T13715	56414-56415	HYPH	denotes	:
T13713	56415-56418	NN	denotes	cre
T13705	56419-56426	NN	denotes	progeny
T13717	56427-56431	VBD	denotes	were
T13716	56432-56440	VBN	denotes	obtained
T13718	56441-56443	IN	denotes	at
T13719	56444-56445	DT	denotes	a
T13720	56446-56450	NN	denotes	rate
T13721	56451-56459	RB	denotes	slightly
T13722	56460-56466	JJR	denotes	higher
T13723	56467-56471	IN	denotes	than
T13724	56472-56473	CD	denotes	1
T13725	56474-56476	IN	denotes	in
T13726	56477-56479	CD	denotes	16
T13727	56479-56480	.	denotes	.
T13728	56480-56702	sentence	denotes	For genotyping of Bmp7 locus we used the following set of primers: Bmp7_ExI_5′ (5′–3′) TCGCCTGCAGCAAGTGACCTCGGGTC; Bmp7_ExI_3′ (5′–3′) TAGGGGTAGGAGAAGCCCTGTCCGTCC and Beta-geo 5′ of 3′ (5′–3′) CTGCATACGCTTGATCCGGCTACCTGC.
T13729	56481-56484	IN	denotes	For
T13731	56485-56495	VBG	denotes	genotyping
T13732	56496-56498	IN	denotes	of
T13733	56499-56503	NN	denotes	Bmp7
T13734	56504-56509	NN	denotes	locus
T13735	56510-56512	PRP	denotes	we
T13730	56513-56517	VBD	denotes	used
T13736	56518-56521	DT	denotes	the
T13738	56522-56531	VBG	denotes	following
T13737	56532-56535	NN	denotes	set
T13739	56536-56538	IN	denotes	of
T13740	56539-56546	NNS	denotes	primers
T13741	56546-56548	:	denotes	:
T13742	56548-56558	NN	denotes	Bmp7_ExI_5
T13744	56558-56559	SYM	denotes	′
T13745	56560-56561	-LRB-	denotes	(
T13746	56561-56562	CD	denotes	5
T13747	56562-56563	SYM	denotes	′
T13748	56563-56564	SYM	denotes	–
T13749	56564-56565	CD	denotes	3
T13750	56565-56566	SYM	denotes	′
T13751	56566-56567	-RRB-	denotes	)
T13743	56568-56594	NN	denotes	TCGCCTGCAGCAAGTGACCTCGGGTC
T13752	56594-56595	:	denotes	;
T13753	56596-56606	NN	denotes	Bmp7_ExI_3
T13755	56606-56607	SYM	denotes	′
T13756	56608-56609	-LRB-	denotes	(
T13757	56609-56610	CD	denotes	5
T13758	56610-56611	SYM	denotes	′
T13759	56611-56612	SYM	denotes	–
T13760	56612-56613	CD	denotes	3
T13761	56613-56614	SYM	denotes	′
T13762	56614-56615	-RRB-	denotes	)
T13754	56616-56643	NN	denotes	TAGGGGTAGGAGAAGCCCTGTCCGTCC
T13763	56644-56647	CC	denotes	and
T13764	56648-56652	NN	denotes	Beta
T13766	56652-56653	HYPH	denotes	-
T13767	56653-56656	NN	denotes	geo
T13765	56657-56658	CD	denotes	5
T13769	56658-56659	SYM	denotes	′
T13770	56660-56662	IN	denotes	of
T13771	56663-56664	CD	denotes	3
T13772	56664-56665	SYM	denotes	′
T13773	56666-56667	-LRB-	denotes	(
T13774	56667-56668	CD	denotes	5
T13775	56668-56669	SYM	denotes	′
T13776	56669-56670	SYM	denotes	–
T13777	56670-56671	CD	denotes	3
T13778	56671-56672	SYM	denotes	′
T13779	56672-56673	-RRB-	denotes	)
T13768	56674-56701	NN	denotes	CTGCATACGCTTGATCCGGCTACCTGC
T13780	56701-56702	.	denotes	.
T13781	56702-56882	sentence	denotes	From a wild-type chromosome Bmp7_ExI_5′ and Bmp7_ExI_3′ amplifies an approximately 423-bp PCR product, while from the lacZ insertion mutant this pair fails to amplify any product.
T13782	56703-56707	IN	denotes	From
T13784	56708-56709	DT	denotes	a
T13786	56710-56714	JJ	denotes	wild
T13788	56714-56715	HYPH	denotes	-
T13787	56715-56719	NN	denotes	type
T13785	56720-56730	NN	denotes	chromosome
T13789	56731-56741	NN	denotes	Bmp7_ExI_5
T13790	56741-56742	SYM	denotes	′
T13791	56743-56746	CC	denotes	and
T13792	56747-56757	NN	denotes	Bmp7_ExI_3
T13793	56757-56758	SYM	denotes	′
T13783	56759-56768	VBZ	denotes	amplifies
T13794	56769-56771	DT	denotes	an
T13796	56772-56785	RB	denotes	approximately
T13797	56786-56789	CD	denotes	423
T13799	56789-56790	HYPH	denotes	-
T13798	56790-56792	NN	denotes	bp
T13800	56793-56796	NN	denotes	PCR
T13795	56797-56804	NN	denotes	product
T13801	56804-56806	,	denotes	,
T13802	56806-56811	IN	denotes	while
T13804	56812-56816	IN	denotes	from
T13805	56817-56820	DT	denotes	the
T13807	56821-56825	NN	denotes	lacZ
T13808	56826-56835	NN	denotes	insertion
T13806	56836-56842	NN	denotes	mutant
T13809	56843-56847	DT	denotes	this
T13810	56848-56852	NN	denotes	pair
T13803	56853-56858	VBZ	denotes	fails
T13811	56859-56861	TO	denotes	to
T13812	56862-56869	VB	denotes	amplify
T13813	56870-56873	DT	denotes	any
T13814	56874-56881	NN	denotes	product
T13815	56881-56882	.	denotes	.
T13816	56882-57048	sentence	denotes	Bmp7_ExI_5′ and Beta-geo 5′ of 3′ (this primer is designed from the Beta-geo cassette) amplifies an approximately 1-kb PCR product from the lacZ inserted chromosome.
T13817	56883-56893	NN	denotes	Bmp7_ExI_5
T13819	56893-56894	SYM	denotes	′
T13820	56895-56898	CC	denotes	and
T13821	56899-56903	NN	denotes	Beta
T13823	56903-56904	HYPH	denotes	-
T13824	56904-56907	NN	denotes	geo
T13822	56908-56909	CD	denotes	5
T13825	56909-56910	SYM	denotes	′
T13826	56911-56913	IN	denotes	of
T13827	56914-56915	CD	denotes	3
T13828	56915-56916	SYM	denotes	′
T13829	56917-56918	-LRB-	denotes	(
T13831	56918-56922	DT	denotes	this
T13832	56923-56929	NN	denotes	primer
T13833	56930-56932	VBZ	denotes	is
T13830	56933-56941	VBN	denotes	designed
T13834	56942-56946	IN	denotes	from
T13835	56947-56950	DT	denotes	the
T13837	56951-56955	NN	denotes	Beta
T13839	56955-56956	HYPH	denotes	-
T13838	56956-56959	NN	denotes	geo
T13836	56960-56968	NN	denotes	cassette
T13840	56968-56969	-RRB-	denotes	)
T13818	56970-56979	VBZ	denotes	amplifies
T13841	56980-56982	DT	denotes	an
T13843	56983-56996	RB	denotes	approximately
T13844	56997-56998	CD	denotes	1
T13846	56998-56999	HYPH	denotes	-
T13845	56999-57001	NN	denotes	kb
T13847	57002-57005	NN	denotes	PCR
T13842	57006-57013	NN	denotes	product
T13848	57014-57018	IN	denotes	from
T13849	57019-57022	DT	denotes	the
T13851	57023-57027	NN	denotes	lacZ
T13852	57028-57036	VBN	denotes	inserted
T13850	57037-57047	NN	denotes	chromosome
T13853	57047-57048	.	denotes	.
T13914	57050-57058	JJ	denotes	Skeletal
T13915	57059-57067	NN	denotes	analysis
T13916	57067-57068	.	denotes	.
T13917	57068-57142	sentence	denotes	Our protocol is an adaptation of the procedure described [56] previously.
T13918	57069-57072	PRP$	denotes	Our
T13919	57073-57081	NN	denotes	protocol
T13920	57082-57084	VBZ	denotes	is
T13921	57085-57087	DT	denotes	an
T13922	57088-57098	NN	denotes	adaptation
T13923	57099-57101	IN	denotes	of
T13924	57102-57105	DT	denotes	the
T13925	57106-57115	NN	denotes	procedure
T13926	57116-57125	VBN	denotes	described
T13927	57126-57127	-LRB-	denotes	[
T13928	57127-57129	CD	denotes	56
T13929	57129-57130	-RRB-	denotes	]
T13930	57131-57141	RB	denotes	previously
T13931	57141-57142	.	denotes	.
T13932	57142-57202	sentence	denotes	Newborn and older animals were killed using carbon dioxide.
T13933	57143-57150	JJ	denotes	Newborn
T13935	57151-57154	CC	denotes	and
T13936	57155-57160	JJR	denotes	older
T13934	57161-57168	NNS	denotes	animals
T13938	57169-57173	VBD	denotes	were
T13937	57174-57180	VBN	denotes	killed
T13939	57181-57186	VBG	denotes	using
T13940	57187-57193	NN	denotes	carbon
T13941	57194-57201	NN	denotes	dioxide
T13942	57201-57202	.	denotes	.
T13943	57202-57321	sentence	denotes	Skin, viscera, and adipose tissues were removed within 2 h after death, and samples were fixed in 95% ethanol for 5 d.
T13944	57203-57207	NN	denotes	Skin
T13946	57207-57209	,	denotes	,
T13947	57209-57216	NN	denotes	viscera
T13948	57216-57218	,	denotes	,
T13949	57218-57221	CC	denotes	and
T13950	57222-57229	NN	denotes	adipose
T13945	57230-57237	NNS	denotes	tissues
T13952	57238-57242	VBD	denotes	were
T13951	57243-57250	VBN	denotes	removed
T13953	57251-57257	IN	denotes	within
T13954	57258-57259	CD	denotes	2
T13955	57260-57261	NNS	denotes	h
T13956	57262-57267	IN	denotes	after
T13957	57268-57273	NN	denotes	death
T13958	57273-57275	,	denotes	,
T13959	57275-57278	CC	denotes	and
T13960	57279-57286	NNS	denotes	samples
T13962	57287-57291	VBD	denotes	were
T13961	57292-57297	VBN	denotes	fixed
T13963	57298-57300	IN	denotes	in
T13964	57301-57303	CD	denotes	95
T13965	57303-57304	NN	denotes	%
T13966	57305-57312	NN	denotes	ethanol
T13967	57313-57316	IN	denotes	for
T13968	57317-57318	CD	denotes	5
T13969	57319-57320	NNS	denotes	d
T13970	57320-57321	.	denotes	.
T13971	57321-57389	sentence	denotes	Samples were then placed in acetone to remove residual fat for 2 d.
T13972	57322-57329	NNS	denotes	Samples
T13974	57330-57334	VBD	denotes	were
T13975	57335-57339	RB	denotes	then
T13973	57340-57346	VBN	denotes	placed
T13976	57347-57349	IN	denotes	in
T13977	57350-57357	NN	denotes	acetone
T13978	57358-57360	TO	denotes	to
T13979	57361-57367	VB	denotes	remove
T13980	57368-57376	JJ	denotes	residual
T13981	57377-57380	NN	denotes	fat
T13982	57381-57384	IN	denotes	for
T13983	57385-57386	CD	denotes	2
T13984	57387-57388	NNS	denotes	d
T13985	57388-57389	.	denotes	.
T13986	57389-57430	sentence	denotes	For E13.5 embryos, skin was not removed.
T13987	57390-57393	IN	denotes	For
T13989	57394-57399	NN	denotes	E13.5
T13990	57400-57407	NNS	denotes	embryos
T13991	57407-57409	,	denotes	,
T13992	57409-57413	NN	denotes	skin
T13993	57414-57417	VBD	denotes	was
T13994	57418-57421	RB	denotes	not
T13988	57422-57429	VBN	denotes	removed
T13995	57429-57430	.	denotes	.
T13996	57430-57580	sentence	denotes	The dehydrated animals were stained with 0.015% w/v Alcian blue and 0.005% w/v Alizarin red in a 1:19 mixture of glacial acetic acid and 70% ethanol.
T13997	57431-57434	DT	denotes	The
T13999	57435-57445	VBN	denotes	dehydrated
T13998	57446-57453	NNS	denotes	animals
T14001	57454-57458	VBD	denotes	were
T14000	57459-57466	VBN	denotes	stained
T14002	57467-57471	IN	denotes	with
T14003	57472-57477	CD	denotes	0.015
T14004	57477-57478	NN	denotes	%
T14006	57479-57480	NN	denotes	w
T14007	57480-57481	SYM	denotes	/
T14005	57481-57482	NN	denotes	v
T14008	57483-57489	JJ	denotes	Alcian
T14009	57490-57494	JJ	denotes	blue
T14010	57495-57498	CC	denotes	and
T14011	57499-57504	CD	denotes	0.005
T14012	57504-57505	NN	denotes	%
T14014	57506-57507	NN	denotes	w
T14015	57507-57508	SYM	denotes	/
T14013	57508-57509	NN	denotes	v
T14016	57510-57518	JJ	denotes	Alizarin
T14017	57519-57522	JJ	denotes	red
T14018	57523-57525	IN	denotes	in
T14019	57526-57527	DT	denotes	a
T14021	57528-57529	CD	denotes	1
T14022	57529-57530	SYM	denotes	:
T14023	57530-57532	CD	denotes	19
T14020	57533-57540	NN	denotes	mixture
T14024	57541-57543	IN	denotes	of
T14025	57544-57551	JJ	denotes	glacial
T14027	57552-57558	JJ	denotes	acetic
T14026	57559-57563	NN	denotes	acid
T14028	57564-57567	CC	denotes	and
T14029	57568-57570	CD	denotes	70
T14030	57570-57571	NN	denotes	%
T14031	57572-57579	NN	denotes	ethanol
T14032	57579-57580	.	denotes	.
T14033	57580-57758	sentence	denotes	The younger animals were stained for 5 h at 37 °C and then overnight at room temperature, while the newborns were stained overnight at 37 °C followed by 2 d at room temperature.
T14034	57581-57584	DT	denotes	The
T14036	57585-57592	JJR	denotes	younger
T14035	57593-57600	NNS	denotes	animals
T14038	57601-57605	VBD	denotes	were
T14037	57606-57613	VBN	denotes	stained
T14039	57614-57617	IN	denotes	for
T14040	57618-57619	CD	denotes	5
T14041	57620-57621	NN	denotes	h
T14042	57622-57624	IN	denotes	at
T14043	57625-57627	CD	denotes	37
T14044	57628-57630	NN	denotes	°C
T14045	57631-57634	CC	denotes	and
T14046	57635-57639	RB	denotes	then
T14048	57640-57649	RB	denotes	overnight
T14047	57650-57652	IN	denotes	at
T14049	57653-57657	NN	denotes	room
T14050	57658-57669	NN	denotes	temperature
T14051	57669-57671	,	denotes	,
T14052	57671-57676	IN	denotes	while
T14054	57677-57680	DT	denotes	the
T14055	57681-57689	NNS	denotes	newborns
T14056	57690-57694	VBD	denotes	were
T14053	57695-57702	VBN	denotes	stained
T14057	57703-57712	RB	denotes	overnight
T14058	57713-57715	IN	denotes	at
T14059	57716-57718	CD	denotes	37
T14060	57719-57721	NN	denotes	°C
T14061	57722-57730	VBN	denotes	followed
T14062	57731-57733	IN	denotes	by
T14063	57734-57735	CD	denotes	2
T14064	57736-57737	NNS	denotes	d
T14065	57738-57740	IN	denotes	at
T14066	57741-57745	NN	denotes	room
T14067	57746-57757	NN	denotes	temperature
T14068	57757-57758	.	denotes	.
T14069	57758-57852	sentence	denotes	The stained skeletons were kept in 1% KOH, with occasional change of solution, until cleared.
T14070	57759-57762	NN	denotes	The
T14072	57763-57770	VBN	denotes	stained
T14071	57771-57780	NNS	denotes	skeletons
T14074	57781-57785	VBD	denotes	were
T14073	57786-57790	VBN	denotes	kept
T14075	57791-57793	IN	denotes	in
T14076	57794-57795	CD	denotes	1
T14077	57795-57796	NN	denotes	%
T14078	57797-57800	NN	denotes	KOH
T14079	57800-57802	,	denotes	,
T14080	57802-57806	IN	denotes	with
T14081	57807-57817	JJ	denotes	occasional
T14082	57818-57824	NN	denotes	change
T14083	57825-57827	IN	denotes	of
T14084	57828-57836	NN	denotes	solution
T14085	57836-57838	,	denotes	,
T14086	57838-57843	IN	denotes	until
T14087	57844-57851	VBN	denotes	cleared
T14088	57851-57852	.	denotes	.
T14089	57852-57928	sentence	denotes	The cleared skeletons were transferred into 100% glycerol and photographed.
T14090	57853-57856	DT	denotes	The
T14092	57857-57864	JJ	denotes	cleared
T14091	57865-57874	NNS	denotes	skeletons
T14094	57875-57879	VBD	denotes	were
T14093	57880-57891	VBN	denotes	transferred
T14095	57892-57896	IN	denotes	into
T14096	57897-57900	CD	denotes	100
T14097	57900-57901	NN	denotes	%
T14098	57902-57910	NN	denotes	glycerol
T14099	57911-57914	CC	denotes	and
T14100	57915-57927	VBN	denotes	photographed
T14101	57927-57928	.	denotes	.
T14125	57930-57938	NN	denotes	Acridine
T14127	57939-57945	JJ	denotes	orange
T14126	57946-57954	NN	denotes	staining
T14128	57955-57958	IN	denotes	for
T14129	57959-57968	JJ	denotes	apoptotic
T14130	57969-57974	NNS	denotes	cells
T14131	57974-57975	.	denotes	.
T14132	57975-58047	sentence	denotes	A working stock of 5 mg/ml acridine orange was diluted 1:10,000 in PBS.
T14133	57976-57977	DT	denotes	A
T14135	57978-57985	VBG	denotes	working
T14134	57986-57991	NN	denotes	stock
T14137	57992-57994	IN	denotes	of
T14138	57995-57996	CD	denotes	5
T14139	57997-57999	NN	denotes	mg
T14141	57999-58000	SYM	denotes	/
T14142	58000-58002	NN	denotes	ml
T14143	58003-58011	NN	denotes	acridine
T14140	58012-58018	NN	denotes	orange
T14144	58019-58022	VBD	denotes	was
T14136	58023-58030	VBN	denotes	diluted
T14145	58031-58032	CD	denotes	1
T14146	58032-58033	SYM	denotes	:
T14147	58033-58039	CD	denotes	10,000
T14148	58040-58042	IN	denotes	in
T14149	58043-58046	NN	denotes	PBS
T14150	58046-58047	.	denotes	.
T14151	58047-58178	sentence	denotes	Dissected fresh embryos were transferred to the working solution of acridine orange and incubated for 30 min at 37 °C in the dark.
T14152	58048-58057	VBN	denotes	Dissected
T14154	58058-58063	JJ	denotes	fresh
T14153	58064-58071	NNS	denotes	embryos
T14156	58072-58076	VBD	denotes	were
T14155	58077-58088	VBN	denotes	transferred
T14157	58089-58091	IN	denotes	to
T14158	58092-58095	DT	denotes	the
T14160	58096-58103	VBG	denotes	working
T14159	58104-58112	NN	denotes	solution
T14161	58113-58115	IN	denotes	of
T14162	58116-58124	NN	denotes	acridine
T14163	58125-58131	NN	denotes	orange
T14164	58132-58135	CC	denotes	and
T14165	58136-58145	VBN	denotes	incubated
T14166	58146-58149	IN	denotes	for
T14167	58150-58152	CD	denotes	30
T14168	58153-58156	NNS	denotes	min
T14169	58157-58159	IN	denotes	at
T14170	58160-58162	CD	denotes	37
T14171	58163-58165	NN	denotes	°C
T14172	58166-58168	IN	denotes	in
T14173	58169-58172	DT	denotes	the
T14174	58173-58177	NN	denotes	dark
T14175	58177-58178	.	denotes	.
T14176	58178-58269	sentence	denotes	Embryos were then washed twice in PBS for 5 min and viewed with a fluorescence microscope.
T14177	58179-58186	NNS	denotes	Embryos
T14179	58187-58191	VBD	denotes	were
T14180	58192-58196	RB	denotes	then
T14178	58197-58203	VBN	denotes	washed
T14181	58204-58209	RB	denotes	twice
T14182	58210-58212	IN	denotes	in
T14183	58213-58216	NN	denotes	PBS
T14184	58217-58220	IN	denotes	for
T14185	58221-58222	CD	denotes	5
T14186	58223-58226	NNS	denotes	min
T14187	58227-58230	CC	denotes	and
T14188	58231-58237	VBN	denotes	viewed
T14189	58238-58242	IN	denotes	with
T14190	58243-58244	DT	denotes	a
T14192	58245-58257	NN	denotes	fluorescence
T14191	58258-58268	NN	denotes	microscope
T14193	58268-58269	.	denotes	.
T14208	58271-58276	JJ	denotes	Whole
T14209	58277-58282	NN	denotes	mount
T14211	58283-58285	FW	denotes	in
T14212	58286-58290	FW	denotes	situ
T14210	58291-58304	NN	denotes	hybridization
T14213	58304-58305	.	denotes	.
T14214	58305-58386	sentence	denotes	In situ hybridization staining on whole embryos was performed as described [57].
T14215	58306-58308	FW	denotes	In
T14216	58309-58313	FW	denotes	situ
T14217	58314-58327	NN	denotes	hybridization
T14218	58328-58336	NN	denotes	staining
T14220	58337-58339	IN	denotes	on
T14221	58340-58345	JJ	denotes	whole
T14222	58346-58353	NNS	denotes	embryos
T14223	58354-58357	VBD	denotes	was
T14219	58358-58367	VBN	denotes	performed
T14224	58368-58370	IN	denotes	as
T14225	58371-58380	VBN	denotes	described
T14226	58381-58382	-LRB-	denotes	[
T14227	58382-58384	CD	denotes	57
T14228	58384-58385	-RRB-	denotes	]
T14229	58385-58386	.	denotes	.
T14230	58386-58440	sentence	denotes	For section in situ hybridizations, please see below.
T14231	58387-58390	IN	denotes	For
T14233	58391-58398	NN	denotes	section
T14235	58399-58401	FW	denotes	in
T14236	58402-58406	FW	denotes	situ
T14234	58407-58421	NNS	denotes	hybridizations
T14237	58421-58423	,	denotes	,
T14238	58423-58429	UH	denotes	please
T14232	58430-58433	VB	denotes	see
T14239	58434-58439	RB	denotes	below
T14240	58439-58440	.	denotes	.
T14445	58442-58451	NN	denotes	Histology
T14446	58451-58453	,	denotes	,
T14447	58453-58460	NN	denotes	section
T14449	58461-58463	FW	denotes	in
T14450	58464-58468	FW	denotes	situ
T14448	58469-58483	NNS	denotes	hybridizations
T14451	58483-58485	,	denotes	,
T14452	58485-58488	CC	denotes	and
T14453	58489-58509	NN	denotes	immunohistochemistry
T14454	58509-58510	.	denotes	.
T14455	58510-58627	sentence	denotes	For histology and section in situ hybridization, samples were fixed in 4% paraformaldehyde in PBS at 4 °C overnight.
T14456	58511-58514	IN	denotes	For
T14458	58515-58524	NN	denotes	histology
T14459	58525-58528	CC	denotes	and
T14460	58529-58536	NN	denotes	section
T14462	58537-58539	FW	denotes	in
T14463	58540-58544	FW	denotes	situ
T14461	58545-58558	NN	denotes	hybridization
T14464	58558-58560	,	denotes	,
T14465	58560-58567	NNS	denotes	samples
T14466	58568-58572	VBD	denotes	were
T14457	58573-58578	VBN	denotes	fixed
T14467	58579-58581	IN	denotes	in
T14468	58582-58583	CD	denotes	4
T14469	58583-58584	NN	denotes	%
T14470	58585-58601	NN	denotes	paraformaldehyde
T14471	58602-58604	IN	denotes	in
T14472	58605-58608	NN	denotes	PBS
T14473	58609-58611	IN	denotes	at
T14474	58612-58613	CD	denotes	4
T14475	58614-58616	NN	denotes	°C
T14476	58617-58626	RB	denotes	overnight
T14477	58626-58627	.	denotes	.
T14478	58627-58768	sentence	denotes	Newborn and older animals were then decalcified in Tris buffer containing 10% EDTA and 7.5% polyvinylpyrrolidone (pH 7.5) at 4 °C for 3 wks.
T14479	58628-58635	JJ	denotes	Newborn
T14481	58636-58639	CC	denotes	and
T14482	58640-58645	JJR	denotes	older
T14480	58646-58653	NNS	denotes	animals
T14484	58654-58658	VBD	denotes	were
T14485	58659-58663	RB	denotes	then
T14483	58664-58675	VBN	denotes	decalcified
T14486	58676-58678	IN	denotes	in
T14487	58679-58683	NN	denotes	Tris
T14488	58684-58690	NN	denotes	buffer
T14489	58691-58701	VBG	denotes	containing
T14490	58702-58704	CD	denotes	10
T14491	58704-58705	NN	denotes	%
T14492	58706-58710	NN	denotes	EDTA
T14493	58711-58714	CC	denotes	and
T14494	58715-58718	CD	denotes	7.5
T14495	58718-58719	NN	denotes	%
T14496	58720-58740	NN	denotes	polyvinylpyrrolidone
T14497	58741-58742	-LRB-	denotes	(
T14498	58742-58744	NN	denotes	pH
T14499	58745-58748	CD	denotes	7.5
T14500	58748-58749	-RRB-	denotes	)
T14501	58750-58752	IN	denotes	at
T14502	58753-58754	CD	denotes	4
T14503	58755-58757	NN	denotes	°C
T14504	58758-58761	IN	denotes	for
T14505	58762-58763	CD	denotes	3
T14506	58764-58767	NNS	denotes	wks
T14507	58767-58768	.	denotes	.
T14508	58768-58875	sentence	denotes	Samples were then dehydrated through a graded ethanol series, cleared in xylene, and embedded in paraffin.
T14509	58769-58776	NNS	denotes	Samples
T14511	58777-58781	VBD	denotes	were
T14512	58782-58786	RB	denotes	then
T14510	58787-58797	VBN	denotes	dehydrated
T14513	58798-58805	IN	denotes	through
T14514	58806-58807	DT	denotes	a
T14516	58808-58814	VBN	denotes	graded
T14517	58815-58822	NN	denotes	ethanol
T14515	58823-58829	NN	denotes	series
T14518	58829-58831	,	denotes	,
T14519	58831-58838	VBN	denotes	cleared
T14520	58839-58841	IN	denotes	in
T14521	58842-58848	NN	denotes	xylene
T14522	58848-58850	,	denotes	,
T14523	58850-58853	CC	denotes	and
T14524	58854-58862	VBN	denotes	embedded
T14525	58863-58865	IN	denotes	in
T14526	58866-58874	NN	denotes	paraffin
T14527	58874-58875	.	denotes	.
T14528	58875-58998	sentence	denotes	8μm sections were collected and stained with hematoxylin and eosin (H & E) or toluidine blue following standard procedure.
T14529	58876-58877	CD	denotes	8
T14530	58877-58879	NN	denotes	μm
T14531	58880-58888	NNS	denotes	sections
T14533	58889-58893	VBD	denotes	were
T14532	58894-58903	VBN	denotes	collected
T14534	58904-58907	CC	denotes	and
T14535	58908-58915	VBN	denotes	stained
T14536	58916-58920	IN	denotes	with
T14537	58921-58932	NN	denotes	hematoxylin
T14538	58933-58936	CC	denotes	and
T14539	58937-58942	NN	denotes	eosin
T14540	58943-58944	-LRB-	denotes	(
T14541	58944-58945	NN	denotes	H
T14542	58946-58947	CC	denotes	&
T14543	58948-58949	NN	denotes	E
T14544	58949-58950	-RRB-	denotes	)
T14545	58951-58953	CC	denotes	or
T14546	58954-58963	NN	denotes	toluidine
T14547	58964-58968	JJ	denotes	blue
T14548	58969-58978	VBG	denotes	following
T14549	58979-58987	JJ	denotes	standard
T14550	58988-58997	NN	denotes	procedure
T14551	58997-58998	.	denotes	.
T14552	58998-59093	sentence	denotes	Section in situ hybridization with digoxigenin labeled probes was performed as described [57].
T14553	58999-59006	NN	denotes	Section
T14555	59007-59009	FW	denotes	in
T14556	59010-59014	FW	denotes	situ
T14554	59015-59028	NN	denotes	hybridization
T14558	59029-59033	IN	denotes	with
T14559	59034-59045	NN	denotes	digoxigenin
T14560	59046-59053	VBN	denotes	labeled
T14561	59054-59060	NNS	denotes	probes
T14562	59061-59064	VBD	denotes	was
T14557	59065-59074	VBN	denotes	performed
T14563	59075-59077	IN	denotes	as
T14564	59078-59087	VBN	denotes	described
T14565	59088-59089	-LRB-	denotes	[
T14566	59089-59091	CD	denotes	57
T14567	59091-59092	-RRB-	denotes	]
T14568	59092-59093	.	denotes	.
T14569	59093-59207	sentence	denotes	Section in situ hybridization with radiolabeled probes was performed as described [58] with a small modification.
T14570	59094-59101	NN	denotes	Section
T14572	59102-59104	FW	denotes	in
T14573	59105-59109	FW	denotes	situ
T14571	59110-59123	NN	denotes	hybridization
T14575	59124-59128	IN	denotes	with
T14576	59129-59141	VBN	denotes	radiolabeled
T14577	59142-59148	NNS	denotes	probes
T14578	59149-59152	VBD	denotes	was
T14574	59153-59162	VBN	denotes	performed
T14579	59163-59165	IN	denotes	as
T14580	59166-59175	VBN	denotes	described
T14581	59176-59177	-LRB-	denotes	[
T14582	59177-59179	CD	denotes	58
T14583	59179-59180	-RRB-	denotes	]
T14584	59181-59185	IN	denotes	with
T14585	59186-59187	DT	denotes	a
T14587	59188-59193	JJ	denotes	small
T14586	59194-59206	NN	denotes	modification
T14588	59206-59207	.	denotes	.
T14589	59207-59303	sentence	denotes	Briefly, sections were deparaffinized in xylene and rehydrated through a graded ethanol series.
T14590	59208-59215	RB	denotes	Briefly
T14592	59215-59217	,	denotes	,
T14593	59217-59225	NNS	denotes	sections
T14594	59226-59230	VBD	denotes	were
T14591	59231-59245	VBN	denotes	deparaffinized
T14595	59246-59248	IN	denotes	in
T14596	59249-59255	NN	denotes	xylene
T14597	59256-59259	CC	denotes	and
T14598	59260-59270	VBN	denotes	rehydrated
T14599	59271-59278	IN	denotes	through
T14600	59279-59280	DT	denotes	a
T14602	59281-59287	VBN	denotes	graded
T14603	59288-59295	NN	denotes	ethanol
T14601	59296-59302	NN	denotes	series
T14604	59302-59303	.	denotes	.
T14605	59303-59676	sentence	denotes	Sections were postfixed in 4% paraformaldehyde in PBS at room temperature for 15 min, digested with 10 μg/ml proteinase K at room temperature for 10 min, and again fixed in 4% paraformaldehyde at room temperature for 10 min and acetylated in the solution containing 0.2% hydrochloric acid, 0.1 M triethanol amine, and 0.25% acetic anhydride at room temperature for 10 min.
T14606	59304-59312	NNS	denotes	Sections
T14608	59313-59317	VBD	denotes	were
T14607	59318-59327	VBN	denotes	postfixed
T14609	59328-59330	IN	denotes	in
T14610	59331-59332	CD	denotes	4
T14611	59332-59333	NN	denotes	%
T14612	59334-59350	NN	denotes	paraformaldehyde
T14613	59351-59353	IN	denotes	in
T14614	59354-59357	NN	denotes	PBS
T14615	59358-59360	IN	denotes	at
T14616	59361-59365	NN	denotes	room
T14617	59366-59377	NN	denotes	temperature
T14618	59378-59381	IN	denotes	for
T14619	59382-59384	CD	denotes	15
T14620	59385-59388	NNS	denotes	min
T14621	59388-59390	,	denotes	,
T14622	59390-59398	VBN	denotes	digested
T14623	59399-59403	IN	denotes	with
T14624	59404-59406	CD	denotes	10
T14625	59407-59409	NN	denotes	μg
T14627	59409-59410	SYM	denotes	/
T14628	59410-59412	NN	denotes	ml
T14629	59413-59423	NN	denotes	proteinase
T14626	59424-59425	NN	denotes	K
T14630	59426-59428	IN	denotes	at
T14631	59429-59433	NN	denotes	room
T14632	59434-59445	NN	denotes	temperature
T14633	59446-59449	IN	denotes	for
T14634	59450-59452	CD	denotes	10
T14635	59453-59456	NNS	denotes	min
T14636	59456-59458	,	denotes	,
T14637	59458-59461	CC	denotes	and
T14638	59462-59467	RB	denotes	again
T14639	59468-59473	VBN	denotes	fixed
T14640	59474-59476	IN	denotes	in
T14641	59477-59478	CD	denotes	4
T14642	59478-59479	NN	denotes	%
T14643	59480-59496	NN	denotes	paraformaldehyde
T14644	59497-59499	IN	denotes	at
T14645	59500-59504	NN	denotes	room
T14646	59505-59516	NN	denotes	temperature
T14647	59517-59520	IN	denotes	for
T14648	59521-59523	CD	denotes	10
T14649	59524-59527	NNS	denotes	min
T14650	59528-59531	CC	denotes	and
T14651	59532-59542	VBN	denotes	acetylated
T14652	59543-59545	IN	denotes	in
T14653	59546-59549	DT	denotes	the
T14654	59550-59558	NN	denotes	solution
T14655	59559-59569	VBG	denotes	containing
T14656	59570-59573	CD	denotes	0.2
T14657	59573-59574	NN	denotes	%
T14659	59575-59587	JJ	denotes	hydrochloric
T14658	59588-59592	NN	denotes	acid
T14660	59592-59594	,	denotes	,
T14661	59594-59597	CD	denotes	0.1
T14662	59598-59599	NN	denotes	M
T14664	59600-59610	NN	denotes	triethanol
T14663	59611-59616	NN	denotes	amine
T14665	59616-59618	,	denotes	,
T14666	59618-59621	CC	denotes	and
T14667	59622-59626	CD	denotes	0.25
T14668	59626-59627	NN	denotes	%
T14670	59628-59634	JJ	denotes	acetic
T14669	59635-59644	NN	denotes	anhydride
T14671	59645-59647	IN	denotes	at
T14672	59648-59652	NN	denotes	room
T14673	59653-59664	NN	denotes	temperature
T14674	59665-59668	IN	denotes	for
T14675	59669-59671	CD	denotes	10
T14676	59672-59675	NNS	denotes	min
T14677	59675-59676	.	denotes	.
T14678	59676-59761	sentence	denotes	Sections were then dehydrated in increasing concentrations of ethanol and air-dried.
T14679	59677-59685	NNS	denotes	Sections
T14681	59686-59690	VBD	denotes	were
T14682	59691-59695	RB	denotes	then
T14680	59696-59706	VBN	denotes	dehydrated
T14683	59707-59709	IN	denotes	in
T14684	59710-59720	VBG	denotes	increasing
T14685	59721-59735	NNS	denotes	concentrations
T14686	59736-59738	IN	denotes	of
T14687	59739-59746	NN	denotes	ethanol
T14688	59747-59750	CC	denotes	and
T14689	59751-59754	NN	denotes	air
T14691	59754-59755	HYPH	denotes	-
T14690	59755-59760	VBN	denotes	dried
T14692	59760-59761	.	denotes	.
T14693	59761-60102	sentence	denotes	Hybridization was performed in a humidified chamber in a solution containing 50% formamide, 10% dextran sulfate, 1× Denhardt's solution, 0.6 M sodium chloride, 0.01 M Tris buffer (pH 7.5), 0.001 M EDTA, 0.05 M dithiothreitol, 0.25% SDS, 200 μg tRNA, and 35S-labeled cRNA probe at the final concentration of 5 × 106 cpm/ml at 55 °C for 16 h.
T14694	59762-59775	NN	denotes	Hybridization
T14696	59776-59779	VBD	denotes	was
T14695	59780-59789	VBN	denotes	performed
T14697	59790-59792	IN	denotes	in
T14698	59793-59794	DT	denotes	a
T14700	59795-59805	VBN	denotes	humidified
T14699	59806-59813	NN	denotes	chamber
T14701	59814-59816	IN	denotes	in
T14702	59817-59818	DT	denotes	a
T14703	59819-59827	NN	denotes	solution
T14704	59828-59838	VBG	denotes	containing
T14705	59839-59841	CD	denotes	50
T14706	59841-59842	NN	denotes	%
T14707	59843-59852	NN	denotes	formamide
T14708	59852-59854	,	denotes	,
T14709	59854-59856	CD	denotes	10
T14710	59856-59857	NN	denotes	%
T14712	59858-59865	NN	denotes	dextran
T14711	59866-59873	NN	denotes	sulfate
T14713	59873-59875	,	denotes	,
T14714	59875-59876	CD	denotes	1
T14716	59876-59877	SYM	denotes	×
T14717	59878-59886	NNP	denotes	Denhardt
T14718	59886-59888	POS	denotes	's
T14715	59889-59897	NN	denotes	solution
T14719	59897-59899	,	denotes	,
T14720	59899-59902	CD	denotes	0.6
T14721	59903-59904	NNS	denotes	M
T14723	59905-59911	NN	denotes	sodium
T14722	59912-59920	NN	denotes	chloride
T14724	59920-59922	,	denotes	,
T14725	59922-59926	CD	denotes	0.01
T14726	59927-59928	NNS	denotes	M
T14728	59929-59933	NN	denotes	Tris
T14727	59934-59940	NN	denotes	buffer
T14729	59941-59942	-LRB-	denotes	(
T14730	59942-59944	NN	denotes	pH
T14731	59945-59948	CD	denotes	7.5
T14732	59948-59949	-RRB-	denotes	)
T14733	59949-59951	,	denotes	,
T14734	59951-59956	CD	denotes	0.001
T14735	59957-59958	NN	denotes	M
T14736	59959-59963	NN	denotes	EDTA
T14737	59963-59965	,	denotes	,
T14738	59965-59969	CD	denotes	0.05
T14739	59970-59971	NNS	denotes	M
T14740	59972-59986	NN	denotes	dithiothreitol
T14741	59986-59988	,	denotes	,
T14742	59988-59992	CD	denotes	0.25
T14743	59992-59993	NN	denotes	%
T14744	59994-59997	NN	denotes	SDS
T14745	59997-59999	,	denotes	,
T14746	59999-60002	CD	denotes	200
T14747	60003-60005	NNS	denotes	μg
T14748	60006-60010	NN	denotes	tRNA
T14749	60010-60012	,	denotes	,
T14750	60012-60015	CC	denotes	and
T14751	60016-60019	NN	denotes	35S
T14753	60019-60020	HYPH	denotes	-
T14754	60020-60027	VBN	denotes	labeled
T14755	60028-60032	NN	denotes	cRNA
T14752	60033-60038	NN	denotes	probe
T14756	60039-60041	IN	denotes	at
T14757	60042-60045	DT	denotes	the
T14759	60046-60051	JJ	denotes	final
T14758	60052-60065	NN	denotes	concentration
T14760	60066-60068	IN	denotes	of
T14761	60069-60070	CD	denotes	5
T14763	60071-60072	SYM	denotes	×
T14762	60073-60076	CD	denotes	106
T14764	60077-60080	NN	denotes	cpm
T14765	60080-60081	SYM	denotes	/
T14766	60081-60083	NN	denotes	ml
T14767	60084-60086	IN	denotes	at
T14768	60087-60089	CD	denotes	55
T14769	60090-60092	NNS	denotes	°C
T14770	60093-60096	IN	denotes	for
T14771	60097-60099	CD	denotes	16
T14772	60100-60101	NNS	denotes	h
T14773	60101-60102	.	denotes	.
T14774	60102-60711	sentence	denotes	After hybridization, sections were washed with a solution containing 5× SSC and 10 mM dithiothreitol at 50 °C for 30 min, incubated in a solution containing 50% formamide, 2× SSC, and 10 mM dithiothreitol at 65 °C for 30 min, treated in a solution containing 10 μg/ml RNase A in TNE [0.01 M Tris buffer (pH 7.6), 0.5 M sodium chloride, and 0.001 M EDTA] at 37 °C for 30 min and then washed with 50% formamide, 2× SSC, and 10 mM dithiothreitol at 65 °C for an additional 30 min followed by two washes with 2× SSC/10 mM dithiothreitol at 65 °C for 30 min, and 0.1× SSC/10 mM dithiothreitol at 65 °C for 30 min.
T14775	60103-60108	IN	denotes	After
T14777	60109-60122	NN	denotes	hybridization
T14778	60122-60124	,	denotes	,
T14779	60124-60132	NNS	denotes	sections
T14780	60133-60137	VBD	denotes	were
T14776	60138-60144	VBN	denotes	washed
T14781	60145-60149	IN	denotes	with
T14782	60150-60151	DT	denotes	a
T14783	60152-60160	NN	denotes	solution
T14784	60161-60171	VBG	denotes	containing
T14785	60172-60173	CD	denotes	5
T14787	60173-60174	SYM	denotes	×
T14786	60175-60178	NN	denotes	SSC
T14788	60179-60182	CC	denotes	and
T14789	60183-60185	CD	denotes	10
T14790	60186-60188	NNS	denotes	mM
T14791	60189-60203	NN	denotes	dithiothreitol
T14792	60204-60206	IN	denotes	at
T14793	60207-60209	CD	denotes	50
T14794	60210-60212	NN	denotes	°C
T14795	60213-60216	IN	denotes	for
T14796	60217-60219	CD	denotes	30
T14797	60220-60223	NNS	denotes	min
T14798	60223-60225	,	denotes	,
T14799	60225-60234	VBN	denotes	incubated
T14800	60235-60237	IN	denotes	in
T14801	60238-60239	DT	denotes	a
T14802	60240-60248	NN	denotes	solution
T14803	60249-60259	VBG	denotes	containing
T14804	60260-60262	CD	denotes	50
T14805	60262-60263	NN	denotes	%
T14806	60264-60273	NN	denotes	formamide
T14807	60273-60275	,	denotes	,
T14808	60275-60276	CD	denotes	2
T14810	60276-60277	SYM	denotes	×
T14809	60278-60281	NN	denotes	SSC
T14811	60281-60283	,	denotes	,
T14812	60283-60286	CC	denotes	and
T14813	60287-60289	CD	denotes	10
T14814	60290-60292	NNS	denotes	mM
T14815	60293-60307	NN	denotes	dithiothreitol
T14816	60308-60310	IN	denotes	at
T14817	60311-60313	CD	denotes	65
T14818	60314-60316	NN	denotes	°C
T14819	60317-60320	IN	denotes	for
T14820	60321-60323	CD	denotes	30
T14821	60324-60327	NNS	denotes	min
T14822	60327-60329	,	denotes	,
T14823	60329-60336	VBN	denotes	treated
T14824	60337-60339	IN	denotes	in
T14825	60340-60341	DT	denotes	a
T14826	60342-60350	NN	denotes	solution
T14827	60351-60361	VBG	denotes	containing
T14828	60362-60364	CD	denotes	10
T14829	60365-60367	NNS	denotes	μg
T14831	60367-60368	SYM	denotes	/
T14832	60368-60370	NN	denotes	ml
T14833	60371-60376	NN	denotes	RNase
T14830	60377-60378	NN	denotes	A
T14834	60379-60381	IN	denotes	in
T14835	60382-60385	NN	denotes	TNE
T14836	60386-60387	-LRB-	denotes	[
T14838	60387-60391	CD	denotes	0.01
T14839	60392-60393	NNS	denotes	M
T14840	60394-60398	NN	denotes	Tris
T14837	60399-60405	NN	denotes	buffer
T14841	60406-60407	-LRB-	denotes	(
T14842	60407-60409	NN	denotes	pH
T14843	60410-60413	CD	denotes	7.6
T14844	60413-60414	-RRB-	denotes	)
T14845	60414-60416	,	denotes	,
T14846	60416-60419	CD	denotes	0.5
T14847	60420-60421	NNS	denotes	M
T14849	60422-60428	NN	denotes	sodium
T14848	60429-60437	NN	denotes	chloride
T14850	60437-60439	,	denotes	,
T14851	60439-60442	CC	denotes	and
T14852	60443-60448	CD	denotes	0.001
T14853	60449-60450	NNS	denotes	M
T14854	60451-60455	NN	denotes	EDTA
T14855	60455-60456	-RRB-	denotes	]
T14856	60457-60459	IN	denotes	at
T14857	60460-60462	CD	denotes	37
T14858	60463-60465	NN	denotes	°C
T14859	60466-60469	IN	denotes	for
T14860	60470-60472	CD	denotes	30
T14861	60473-60476	NNS	denotes	min
T14862	60477-60480	CC	denotes	and
T14863	60481-60485	RB	denotes	then
T14864	60486-60492	VBN	denotes	washed
T14865	60493-60497	IN	denotes	with
T14866	60498-60500	CD	denotes	50
T14867	60500-60501	NN	denotes	%
T14868	60502-60511	NN	denotes	formamide
T14869	60511-60513	,	denotes	,
T14870	60513-60514	CD	denotes	2
T14872	60514-60515	SYM	denotes	×
T14871	60516-60519	NN	denotes	SSC
T14873	60519-60521	,	denotes	,
T14874	60521-60524	CC	denotes	and
T14875	60525-60527	CD	denotes	10
T14876	60528-60530	NNS	denotes	mM
T14877	60531-60545	NN	denotes	dithiothreitol
T14878	60546-60548	IN	denotes	at
T14879	60549-60551	CD	denotes	65
T14880	60552-60554	NN	denotes	°C
T14881	60555-60558	IN	denotes	for
T14882	60559-60561	DT	denotes	an
T14884	60562-60572	JJ	denotes	additional
T14885	60573-60575	CD	denotes	30
T14883	60576-60579	NNS	denotes	min
T14886	60580-60588	VBN	denotes	followed
T14887	60589-60591	IN	denotes	by
T14888	60592-60595	CD	denotes	two
T14889	60596-60602	NNS	denotes	washes
T14890	60603-60607	IN	denotes	with
T14891	60608-60609	CD	denotes	2
T14893	60609-60610	SYM	denotes	×
T14892	60611-60614	NN	denotes	SSC
T14894	60614-60615	SYM	denotes	/
T14896	60615-60617	CD	denotes	10
T14897	60618-60620	NN	denotes	mM
T14895	60621-60635	NN	denotes	dithiothreitol
T14898	60636-60638	IN	denotes	at
T14899	60639-60641	CD	denotes	65
T14900	60642-60644	NN	denotes	°C
T14901	60645-60648	IN	denotes	for
T14902	60649-60651	CD	denotes	30
T14903	60652-60655	NNS	denotes	min
T14904	60655-60657	,	denotes	,
T14905	60657-60660	CC	denotes	and
T14906	60661-60664	CD	denotes	0.1
T14908	60664-60665	SYM	denotes	×
T14907	60666-60669	NN	denotes	SSC
T14909	60669-60670	SYM	denotes	/
T14911	60670-60672	CD	denotes	10
T14912	60673-60675	NN	denotes	mM
T14910	60676-60690	NN	denotes	dithiothreitol
T14913	60691-60693	IN	denotes	at
T14914	60694-60696	CD	denotes	65
T14915	60697-60699	NN	denotes	°C
T14916	60700-60703	IN	denotes	for
T14917	60704-60706	CD	denotes	30
T14918	60707-60710	NN	denotes	min
T14919	60710-60711	.	denotes	.
T14920	60711-60827	sentence	denotes	To visualize the signal, sections were dipped into NTB-2 emulsion (Kodak, http://www.Kodak.com) and placed at 4 °C.
T14921	60712-60714	TO	denotes	To
T14922	60715-60724	VB	denotes	visualize
T14924	60725-60728	DT	denotes	the
T14925	60729-60735	NN	denotes	signal
T14926	60735-60737	,	denotes	,
T14927	60737-60745	NNS	denotes	sections
T14928	60746-60750	VBD	denotes	were
T14923	60751-60757	VBN	denotes	dipped
T14929	60758-60762	IN	denotes	into
T14930	60763-60766	NN	denotes	NTB
T14932	60766-60767	HYPH	denotes	-
T14933	60767-60768	CD	denotes	2
T14931	60769-60777	NN	denotes	emulsion
T14934	60778-60779	-LRB-	denotes	(
T14935	60779-60784	NNP	denotes	Kodak
T14936	60784-60786	,	denotes	,
T14937	60786-60806	NN	denotes	http://www.Kodak.com
T14938	60806-60807	-RRB-	denotes	)
T14939	60808-60811	CC	denotes	and
T14940	60812-60818	VBN	denotes	placed
T14941	60819-60821	IN	denotes	at
T14942	60822-60823	CD	denotes	4
T14943	60824-60826	NN	denotes	°C
T14944	60826-60827	.	denotes	.
T14945	60827-60884	sentence	denotes	After developing, sections were counterstained with H&E.
T14946	60828-60833	IN	denotes	After
T14948	60834-60844	VBG	denotes	developing
T14949	60844-60846	,	denotes	,
T14950	60846-60854	NNS	denotes	sections
T14951	60855-60859	VBD	denotes	were
T14947	60860-60874	VBN	denotes	counterstained
T14952	60875-60879	IN	denotes	with
T14953	60880-60881	NN	denotes	H
T14954	60881-60882	CC	denotes	&
T14955	60882-60883	NN	denotes	E
T14956	60883-60884	.	denotes	.
T14957	60884-61022	sentence	denotes	The 35S-labeled cRNA probes were transcribed from plasmids encoding type I collagen [59], runx2 [60], osteopontin [61], and osterix [46].
T14958	60885-60888	DT	denotes	The
T14960	60889-60892	NN	denotes	35S
T14962	60892-60893	HYPH	denotes	-
T14961	60893-60900	VBN	denotes	labeled
T14963	60901-60905	NN	denotes	cRNA
T14959	60906-60912	NNS	denotes	probes
T14965	60913-60917	VBD	denotes	were
T14964	60918-60929	VBN	denotes	transcribed
T14966	60930-60934	IN	denotes	from
T14967	60935-60943	NNS	denotes	plasmids
T14968	60944-60952	VBG	denotes	encoding
T14969	60953-60957	NN	denotes	type
T14971	60958-60959	CD	denotes	I
T14970	60960-60968	NN	denotes	collagen
T14972	60969-60970	-LRB-	denotes	[
T14973	60970-60972	CD	denotes	59
T14974	60972-60973	-RRB-	denotes	]
T14975	60973-60975	,	denotes	,
T14976	60975-60980	NN	denotes	runx2
T14977	60981-60982	-LRB-	denotes	[
T14978	60982-60984	CD	denotes	60
T14979	60984-60985	-RRB-	denotes	]
T14980	60985-60987	,	denotes	,
T14981	60987-60998	NN	denotes	osteopontin
T14982	60999-61000	-LRB-	denotes	[
T14983	61000-61002	CD	denotes	61
T14984	61002-61003	-RRB-	denotes	]
T14985	61003-61005	,	denotes	,
T14986	61005-61008	CC	denotes	and
T14987	61009-61016	NN	denotes	osterix
T14988	61017-61018	-LRB-	denotes	[
T14989	61018-61020	CD	denotes	46
T14990	61020-61021	-RRB-	denotes	]
T14991	61021-61022	.	denotes	.
T14992	61022-61163	sentence	denotes	Sense and antisense probes were synthesized from linearized plasmids using a Riboprobe Combination System (Promega, http://www.promega.com).
T14993	61023-61028	NN	denotes	Sense
T14995	61029-61032	CC	denotes	and
T14996	61033-61042	NN	denotes	antisense
T14994	61043-61049	NNS	denotes	probes
T14998	61050-61054	VBD	denotes	were
T14997	61055-61066	VBN	denotes	synthesized
T14999	61067-61071	IN	denotes	from
T15000	61072-61082	VBN	denotes	linearized
T15001	61083-61091	NNS	denotes	plasmids
T15002	61092-61097	VBG	denotes	using
T15003	61098-61099	DT	denotes	a
T15005	61100-61109	NNP	denotes	Riboprobe
T15006	61110-61121	NNP	denotes	Combination
T15004	61122-61128	NNP	denotes	System
T15007	61129-61130	-LRB-	denotes	(
T15008	61130-61137	NNP	denotes	Promega
T15009	61137-61139	,	denotes	,
T15010	61139-61161	NN	denotes	http://www.promega.com
T15011	61161-61162	-RRB-	denotes	)
T15012	61162-61163	.	denotes	.
R1	T155	T153	prep	of,Analysis
R10	T164	T161	conj	BMP7,BMP4
R100	T263	T264	det	these,issues
R1000	T1990	T1987	conj	BMP7,BMP4
R1001	T1991	T1982	prep	in,remains
R1002	T1912	T1913	nsubj	mice,show
R1003	T1992	T1993	det	this,role
R1004	T1993	T1991	pobj	role,in
R1005	T1994	T1995	det	a,possibility
R1006	T1913	T1900	conj	show,produce
R1007	T1995	T1982	attr	possibility,remains
R1008	T1996	T1955	punct	.,seem
R1009	T1914	T1912	acl	harboring,mice
R101	T264	T261	dobj	issues,circumvent
R1010	T1998	T1999	det	A,aspect
R1011	T1999	T2001	nsubj	aspect,is
R1012	T1915	T1916	det	a,deletion
R1013	T2000	T1999	amod	third,aspect
R1014	T2002	T1999	prep	of,aspect
R1015	T1916	T1914	dobj	deletion,harboring
R1016	T2003	T2004	compound	limb,patterning
R1017	T2004	T2002	pobj	patterning,of
R1018	T1917	T1916	amod	conditional,deletion
R1019	T2005	T2006	prep	in,implicated
R102	T265	T262	punct	", ",constructed
R1020	T2006	T1999	relcl	implicated,aspect
R1021	T2007	T2005	pobj	which,in
R1022	T1918	T1916	prep	of,deletion
R1023	T2008	T2006	nsubjpass	BMP2,implicated
R1024	T2009	T2008	punct	", ",BMP2
R1025	T2010	T2008	conj	BMP4,BMP2
R1026	T1919	T1918	pobj	Bmp4,of
R1027	T2011	T2010	punct	", ",BMP4
R1028	T2012	T2010	cc	and,BMP4
R1029	T1920	T1914	prep	in,harboring
R103	T266	T262	nsubj	we,constructed
R1030	T2013	T2010	conj	BMP7,BMP4
R1031	T2014	T2006	aux	have,implicated
R1032	T2015	T2006	auxpass	been,implicated
R1033	T1921	T1922	det	the,mesenchyme
R1034	T2016	T2001	prep	in,is
R1035	T2017	T2018	det	the,death
R1036	T1922	T1920	pobj	mesenchyme,in
R1037	T2018	T2016	pobj	death,in
R1038	T2019	T2018	amod	apoptotic,death
R1039	T2020	T2018	prep	of,death
R104	T267	T262	aux	have,constructed
R1040	T2021	T2022	det	the,tissue
R1041	T2022	T2020	pobj	tissue,of
R1042	T1923	T1924	compound	limb,bud
R1043	T2023	T2022	amod	interdigital,tissue
R1044	T2024	T2001	punct	.,is
R1045	T1924	T1922	compound	bud,mesenchyme
R1046	T2026	T2027	prep	For,demonstrates
R1047	T2028	T2026	pobj	example,For
R1048	T1925	T1913	advmod	also,show
R1049	T2029	T2027	punct	", ",demonstrates
R105	T268	T269	det	a,series
R1050	T2030	T2031	amod	ectopic,application
R1051	T2031	T2027	nsubj	application,demonstrates
R1052	T1926	T1913	aux	do,show
R1053	T2032	T2031	prep	of,application
R1054	T2033	T2034	compound	BMP,antagonists
R1055	T2034	T2032	pobj	antagonists,of
R1056	T1927	T1913	neg	not,show
R1057	T2035	T2036	mark	that,is
R1058	T2036	T2027	ccomp	is,demonstrates
R1059	T2037	T2038	compound	BMP,signaling
R106	T269	T262	dobj	series,constructed
R1060	T1928	T1913	dobj	evidence,show
R1061	T2038	T2036	nsubj	signaling,is
R1062	T2039	T2036	acomp	necessary,is
R1063	T1929	T1928	prep	of,evidence
R1064	T2040	T2039	prep	for,necessary
R1065	T2041	T2042	det	this,process
R1066	T2042	T2040	pobj	process,for
R1067	T1930	T1931	amod	anterior,transformation
R1068	T2043	T2036	punct	", ",is
R1069	T2044	T2036	cc	and,is
R107	T270	T269	prep	of,series
R1070	T2045	T2046	prep	in,occurs
R1071	T1931	T1929	pobj	transformation,of
R1072	T2046	T2036	conj	occurs,is
R1073	T2047	T2048	poss	its,absence
R1074	T2048	T2045	pobj	absence,in
R1075	T1932	T1931	compound	digit,transformation
R1076	T2049	T2046	punct	", ",occurs
R1077	T2050	T2046	nsubj	webbing,occurs
R1078	T1933	T1934	punct	[,16
R1079	T2051	T2052	punct	[,17
R108	T271	T272	compound	mouse,strains
R1080	T2052	T2046	parataxis	17,occurs
R1081	T2053	T2054	punct	–,19
R1082	T1934	T1928	parataxis	16,evidence
R1083	T2054	T2052	prep	19,17
R1084	T2055	T2052	punct	],17
R1085	T2056	T2027	punct	),demonstrates
R1086	T1935	T1934	punct	],16
R1087	T2057	T2027	punct	.,demonstrates
R1088	T2059	T2060	compound	BMP,activity
R1089	T2060	T2061	nsubj	activity,plays
R109	T272	T270	pobj	strains,of
R1090	T1936	T1913	punct	", ",show
R1091	T2062	T2061	advmod	also,plays
R1092	T1937	T1938	mark	as,predicted
R1093	T2063	T2064	det	an,role
R1094	T2064	T2061	dobj	role,plays
R1095	T1938	T1913	advcl	predicted,show
R1096	T2065	T2064	amod	indirect,role
R1097	T2066	T2061	prep	in,plays
R1098	T1939	T1938	aux	would,predicted
R1099	T2067	T2068	compound	limb,patterning
R11	T165	T157	prep	in,Roles
R110	T273	T272	acl	lacking,strains
R1100	T1940	T1938	aux	have,predicted
R1101	T1941	T1938	auxpass	been,predicted
R1102	T2068	T2066	pobj	patterning,in
R1103	T2069	T2061	prep	as,plays
R1104	T2070	T2071	det	a,component
R1105	T1942	T1943	mark	if,acted
R1106	T2071	T2069	pobj	component,as
R1107	T2072	T2071	amod	key,component
R1108	T1943	T1938	advcl	acted,predicted
R1109	T2073	T2071	prep	of,component
R111	T274	T273	dobj	one,lacking
R1110	T2074	T2075	det	a,loop
R1111	T2075	T2073	pobj	loop,of
R1112	T1944	T1943	nsubj	BMP4,acted
R1113	T2076	T2075	compound	feedback,loop
R1114	T2077	T2075	prep	between,loop
R1115	T2078	T2079	compound	SHH,production
R1116	T1945	T1943	prep	as,acted
R1117	T2079	T2077	pobj	production,between
R1118	T2080	T2079	prep	in,production
R1119	T1946	T1947	det	an,regulator
R112	T275	T274	cc	or,one
R1120	T1947	T1945	pobj	regulator,as
R1121	T1948	T1947	amod	interdigital,regulator
R1122	T1949	T1947	prep	of,regulator
R1123	T2081	T2082	det	the,bud
R1124	T2082	T2080	pobj	bud,in
R1125	T2083	T2082	amod	posterior,bud
R1126	T1950	T1951	compound	digit,morphology
R1127	T2084	T2082	compound	limb,bud
R1128	T2085	T2079	cc	and,production
R1129	T2086	T2087	compound	fibroblast,production
R113	T276	T274	conj	more,one
R1130	T1951	T1949	pobj	morphology,of
R1131	T2087	T2079	conj	production,production
R1132	T2088	T2089	compound	growth,factor
R1133	T2089	T2087	compound	factor,production
R1134	T1952	T1913	punct	.,show
R1135	T2090	T2087	prep	in,production
R1136	T2091	T2092	det	the,ridge
R1137	T2092	T2090	pobj	ridge,in
R1138	T1954	T1955	prep	In,seem
R1139	T2093	T2092	amod	overlying,ridge
R114	T277	T274	prep	of,one
R1140	T2094	T2092	amod	apical,ridge
R1141	T2095	T2092	amod	ectodermal,ridge
R1142	T2096	T2092	punct	(,ridge
R1143	T2097	T2092	appos	AER,ridge
R1144	T2098	T2061	punct	),plays
R1145	T2099	T2061	punct	.,plays
R1146	T1956	T1957	det	the,light
R1147	T2101	T2102	compound	SHH,activity
R1148	T2102	T2103	nsubj	activity,leads
R1149	T1957	T1954	pobj	light,In
R115	T278	T279	det	these,BMPs
R1150	T2104	T2103	prep	to,leads
R1151	T2105	T2106	det	the,upregulation
R1152	T2106	T2104	pobj	upregulation,to
R1153	T1958	T1957	prep	of,light
R1154	T2107	T2106	prep	of,upregulation
R1155	T2108	T2109	det	the,Gremlin
R1156	T1959	T1960	det	these,observations
R1157	T2109	T2107	pobj	Gremlin,of
R1158	T1960	T1958	pobj	observations,of
R1159	T2110	T2109	compound	BMP,Gremlin
R116	T279	T277	pobj	BMPs,of
R1160	T2111	T2109	compound	antagonist,Gremlin
R1161	T2112	T2103	prep	in,leads
R1162	T1961	T1955	punct	", ",seem
R1163	T2113	T2114	det	the,mesenchyme
R1164	T2114	T2112	pobj	mesenchyme,in
R1165	T1962	T1955	nsubj	it,seem
R1166	T2115	T2116	punct	[,21
R1167	T2116	T2103	parataxis	21,leads
R1168	T2117	T2116	nummod	20,21
R1169	T1963	T1955	aux	would,seem
R117	T280	T262	punct	", ",constructed
R1170	T2118	T2116	punct	",",21
R1171	T2119	T2116	punct	],21
R1172	T2120	T2103	punct	.,leads
R1173	T1964	T1955	oprd	unlikely,seem
R1174	T2122	T2123	nsubj	This,prevents
R1175	T1965	T1966	mark	that,are
R1176	T2124	T2123	punct	", ",prevents
R1177	T2125	T2123	prep	in,prevents
R1178	T2126	T2125	pobj	turn,in
R1179	T1966	T1955	ccomp	are,seem
R118	T281	T262	advcl	using,constructed
R1180	T2127	T2123	punct	", ",prevents
R1181	T2128	T2123	dobj	BMPs,prevents
R1182	T2129	T2123	prep	from,prevents
R1183	T1967	T1968	amod	quantitative,differences
R1184	T2130	T2129	pcomp	downregulating,from
R1185	T2131	T2132	nmod	fibroblast,factor
R1186	T2132	T2134	nmod	factor,production
R1187	T1968	T1966	nsubj	differences,are
R1188	T2133	T2132	nmod	growth,factor
R1189	T2134	T2130	dobj	production,downregulating
R119	T282	T283	amod	conditional,alleles
R1190	T1969	T1968	prep	in,differences
R1191	T2135	T2132	punct	(,factor
R1192	T2136	T2132	appos	FGF,factor
R1193	T2137	T2134	punct	),production
R1194	T2138	T2130	prep	in,downregulating
R1195	T2139	T2140	det	the,AER
R1196	T2140	T2138	pobj	AER,in
R1197	T1970	T1969	pobj	levels,in
R1198	T2141	T2142	punct	[,22
R1199	T2142	T2123	parataxis	22,prevents
R12	T166	T167	compound	Limb,Patterning
R120	T283	T281	dobj	alleles,using
R1200	T2143	T2142	nummod	17,22
R1201	T2144	T2142	punct	",",22
R1202	T1971	T1970	prep	of,levels
R1203	T2145	T2142	punct	],22
R1204	T2146	T2123	cc	and,prevents
R1205	T1972	T1973	amod	interdigital,signal
R1206	T2147	T2123	conj	maintains,prevents
R1207	T1973	T1971	pobj	signal,of
R1208	T2148	T2149	det	the,integrity
R1209	T2149	T2147	dobj	integrity,maintains
R121	T284	T281	prep	in,using
R1210	T2150	T2149	prep	of,integrity
R1211	T1974	T1973	compound	BMP,signal
R1212	T2151	T2152	det	the,AER
R1213	T2186	T2182	prep	from,removal
R1214	T2152	T2150	pobj	AER,of
R1215	T2153	T2152	appos	itself,AER
R1216	T2154	T2155	punct	[,23
R1217	T2155	T2147	parataxis	23,maintains
R1218	T2187	T2188	det	the,mesenchyme
R1219	T2156	T2155	punct	],23
R122	T285	T286	det	the,case
R1220	T2157	T2123	punct	.,prevents
R1221	T2188	T2186	pobj	mesenchyme,from
R1222	T2159	T2160	compound	FGF,signaling
R1223	T2160	T2161	nsubj	signaling,feeds
R1224	T2189	T2188	amod	posterior,mesenchyme
R1225	T2162	T2161	advmod	back,feeds
R1226	T2190	T2188	compound	limb,mesenchyme
R1227	T2163	T2164	aux	to,maintain
R1228	T2164	T2161	advcl	maintain,feeds
R1229	T2165	T2166	compound	SHH,production
R123	T286	T284	pobj	case,in
R1230	T2191	T2179	prep	in,results
R1231	T2166	T2164	dobj	production,maintain
R1232	T2167	T2164	prep	in,maintain
R1233	T2168	T2169	det	the,mesenchyme
R1234	T2192	T2193	det	a,persistence
R1235	T2169	T2167	pobj	mesenchyme,in
R1236	T2170	T2169	amod	posterior,mesenchyme
R1237	T2193	T2191	pobj	persistence,in
R1238	T2171	T2172	punct	[,24
R1239	T2172	T2161	parataxis	24,feeds
R124	T287	T286	prep	of,case
R1240	T2173	T2172	nummod	8,24
R1241	T2194	T2193	prep	of,persistence
R1242	T2174	T2172	punct	",",24
R1243	T2175	T2172	punct	],24
R1244	T2176	T2161	punct	.,feeds
R1245	T2178	T2179	advmod	Thus,results
R1246	T2195	T2196	det	the,AER
R1247	T2180	T2179	punct	", ",results
R1248	T2181	T2182	amod	conditional,removal
R1249	T2182	T2179	nsubj	removal,results
R125	T288	T287	pobj	Bmp2,of
R1250	T2183	T2182	prep	of,removal
R1251	T2196	T2194	pobj	AER,of
R1252	T2184	T2185	compound	BMP4,activity
R1253	T2185	T2183	pobj	activity,of
R1254	T2197	T2193	punct	", ",persistence
R1255	T2198	T2199	amod	expanded,signaling
R1256	T2199	T2193	conj	signaling,persistence
R1257	T2292	T2289	advcl	introduced,take
R1258	T2200	T2199	compound	SHH,signaling
R1259	T2293	T2292	nsubjpass	noggin,introduced
R126	T289	T288	cc	and,Bmp2
R1260	T2294	T2293	punct	", ",noggin
R1261	T2201	T2199	punct	", ",signaling
R1262	T2295	T2296	det	a,inhibitor
R1263	T2296	T2293	appos	inhibitor,noggin
R1264	T2202	T2199	cc	and,signaling
R1265	T2297	T2296	amod	potent,inhibitor
R1266	T2298	T2296	compound	BMP,inhibitor
R1267	T2203	T2204	amod	consequent,polydactyly
R1268	T2299	T2292	punct	", ",introduced
R1269	T2204	T2199	conj	polydactyly,signaling
R127	T290	T288	conj	Bmp4,Bmp2
R1270	T2300	T2292	auxpass	is,introduced
R1271	T2301	T2292	prep	in,introduced
R1272	T2302	T2303	amod	early,stage
R1273	T2205	T2204	amod	preaxial,polydactyly
R1274	T2303	T2304	compound	stage,buds
R1275	T2304	T2301	pobj	buds,in
R1276	T2305	T2304	compound	chick,buds
R1277	T2206	T2205	cc	and,preaxial
R1278	T2306	T2304	compound	limb,buds
R1279	T2307	T2308	amod	prior,to
R128	T291	T292	aux	to,remove
R1280	T2308	T2292	prep	to,introduced
R1281	T2207	T2205	conj	postaxial,preaxial
R1282	T2309	T2308	pobj	skeletogenesis,to
R1283	T2310	T2289	punct	", ",take
R1284	T2208	T2209	punct	[,16
R1285	T2311	T2312	amod	mesenchymal,condensation
R1286	T2312	T2289	nsubj	condensation,take
R1287	T2313	T2289	aux	does,take
R1288	T2209	T2179	parataxis	16,results
R1289	T2314	T2289	neg	not,take
R129	T292	T281	advcl	remove,using
R1290	T2315	T2289	dobj	place,take
R1291	T2316	T2317	punct	[,28
R1292	T2210	T2209	punct	],16
R1293	T2317	T2289	parataxis	28,take
R1294	T2318	T2317	nummod	27,28
R1295	T2211	T2179	punct	.,results
R1296	T2319	T2317	punct	",",28
R1297	T2320	T2317	punct	],28
R1298	T2213	T2214	prep	At,believed
R1299	T2321	T2289	punct	.,take
R13	T167	T165	pobj	Patterning,in
R130	T293	T292	dobj	them,remove
R1300	T2323	T2324	advcl	Taken,implicate
R1301	T2215	T2216	amod	later,stages
R1302	T2325	T2323	advmod	together,Taken
R1303	T2326	T2324	punct	", ",implicate
R1304	T2327	T2328	det	these,studies
R1305	T2216	T2213	pobj	stages,At
R1306	T2328	T2324	nsubj	studies,implicate
R1307	T2329	T2324	advmod	strongly,implicate
R1308	T2330	T2331	compound	BMP,signaling
R1309	T2217	T2216	prep	of,stages
R131	T294	T292	advmod	specifically,remove
R1310	T2331	T2324	dobj	signaling,implicate
R1311	T2332	T2324	prep	as,implicate
R1312	T2218	T2219	compound	limb,development
R1313	T2333	T2332	amod	necessary,as
R1314	T2334	T2333	prep	for,necessary
R1315	T2335	T2336	amod	mesenchymal,condensations
R1316	T2219	T2217	pobj	development,of
R1317	T2336	T2334	pobj	condensations,for
R1318	T2337	T2336	cc	and,condensations
R1319	T2220	T2214	punct	", ",believed
R132	T295	T292	prep	from,remove
R1320	T2338	T2339	det	the,initiation
R1321	T2339	T2336	conj	initiation,condensations
R1322	T2340	T2339	prep	of,initiation
R1323	T2221	T2222	compound	BMP,activity
R1324	T2341	T2340	pobj	chondrogenesis,of
R1325	T2342	T2324	punct	.,implicate
R1326	T2222	T2214	nsubjpass	activity,believed
R1327	T2344	T2345	advmod	However,are
R1328	T2223	T2214	auxpass	is,believed
R1329	T2346	T2345	punct	", ",are
R133	T296	T297	det	the,mesenchyme
R1330	T2347	T2348	det	the,phenotypes
R1331	T2224	T2214	advmod	also,believed
R1332	T2348	T2345	nsubj	phenotypes,are
R1333	T2349	T2348	prep	in,phenotypes
R1334	T2350	T2351	det	these,experiments
R1335	T2225	T2226	aux	to,play
R1336	T2351	T2349	pobj	experiments,in
R1337	T2352	T2345	acomp	severe,are
R1338	T2353	T2345	punct	", ",are
R1339	T2226	T2214	xcomp	play,believed
R134	T297	T295	pobj	mesenchyme,from
R1340	T2354	T2345	advcl	causing,are
R1341	T2355	T2356	preconj	either,chondrogenesis
R1342	T2356	T2354	dobj	chondrogenesis,causing
R1343	T2227	T2228	amod	critical,roles
R1344	T2228	T2226	dobj	roles,play
R1345	T2357	T2356	amod	global,chondrogenesis
R1346	T2229	T2226	prep	in,play
R1347	T2358	T2356	cc	or,chondrogenesis
R1348	T2359	T2356	conj	absence,chondrogenesis
R1349	T2360	T2359	prep	of,absence
R135	T298	T299	compound	limb,bud
R1350	T2361	T2360	pobj	chondrogenesis,of
R1351	T2362	T2354	punct	", ",causing
R1352	T2363	T2354	prep	in,causing
R1353	T2230	T2229	pobj	skeletogenesis,in
R1354	T2364	T2365	det	the,experiments
R1355	T2365	T2363	pobj	experiments,in
R1356	T2231	T2214	punct	.,believed
R1357	T2366	T2365	nmod	gain,experiments
R1358	T2367	T2366	punct	-,gain
R1359	T2368	T2366	cc	and,gain
R136	T299	T297	compound	bud,mesenchyme
R1360	T2233	T2234	nsubj	Activation,shown
R1361	T2369	T2366	conj	loss,gain
R1362	T2370	T2366	punct	-,gain
R1363	T2371	T2366	prep	of,gain
R1364	T2235	T2233	cc	and,Activation
R1365	T2372	T2371	punct	-,of
R1366	T2373	T2371	pobj	function,of
R1367	T2374	T2354	punct	", ",causing
R1368	T2375	T2354	advmod	respectively,causing
R1369	T2236	T2237	amod	dominant,negative
R137	T300	T262	punct	.,constructed
R1370	T2376	T2345	punct	.,are
R1371	T2378	T2379	det	These,defects
R1372	T2237	T2239	amod	negative,experiments
R1373	T2379	T2381	nsubj	defects,preclude
R1374	T2380	T2379	amod	severe,defects
R1375	T2238	T2237	punct	-,negative
R1376	T2382	T2379	prep	during,defects
R1377	T2383	T2384	det	the,stages
R1378	T2384	T2382	pobj	stages,during
R1379	T2239	T2233	conj	experiments,Activation
R138	T302	T303	advcl	Contrary,indicate
R1380	T2385	T2384	amod	early,stages
R1381	T2386	T2384	prep	of,stages
R1382	T2240	T2239	prep	in,experiments
R1383	T2387	T2386	pobj	skeletogenesis,of
R1384	T2388	T2381	dobj	investigation,preclude
R1385	T2389	T2388	prep	of,investigation
R1386	T2241	T2242	det	the,chick
R1387	T2390	T2391	det	any,roles
R1388	T2391	T2389	pobj	roles,of
R1389	T2392	T2391	amod	specific,roles
R139	T304	T302	prep	to,Contrary
R1390	T2242	T2240	pobj	chick,in
R1391	T2393	T2391	prep	for,roles
R1392	T2394	T2393	pobj	BMPs,for
R1393	T2243	T2234	aux	have,shown
R1394	T2395	T2381	prep	in,preclude
R1395	T2396	T2397	amod	later,events
R1396	T2397	T2395	pobj	events,in
R1397	T2244	T2245	mark	that,is
R1398	T2245	T2234	ccomp	is,shown
R1399	T2246	T2245	csubj	signaling,is
R14	T168	T167	cc	and,Patterning
R140	T305	T306	amod	earlier,suggestions
R1400	T2247	T2246	prep	through,signaling
R1401	T2398	T2397	prep	in,events
R1402	T2399	T2400	compound	cartilage,differentiation
R1403	T2400	T2398	pobj	differentiation,in
R1404	T2401	T2400	punct	", ",differentiation
R1405	T2402	T2403	compound	bone,formation
R1406	T2248	T2249	det	the,receptor
R1407	T2403	T2400	conj	formation,differentiation
R1408	T2404	T2403	punct	", ",formation
R1409	T2405	T2403	cc	and,formation
R141	T306	T304	pobj	suggestions,to
R1410	T2249	T2247	pobj	receptor,through
R1411	T2406	T2407	compound	bone,metabolism
R1412	T2407	T2403	conj	metabolism,formation
R1413	T2250	T2251	compound	BMPR,IB
R1414	T2251	T2249	compound	IB,receptor
R1415	T2408	T2409	punct	[,29
R1416	T2409	T2381	parataxis	29,preclude
R1417	T2252	T2251	punct	-,IB
R1418	T2410	T2409	punct	],29
R1419	T2411	T2381	punct	.,preclude
R142	T307	T303	punct	", ",indicate
R1420	T2253	T2245	acomp	necessary,is
R1421	T2413	T2414	advmod	Conversely,displayed
R1422	T2254	T2253	cc	and,necessary
R1423	T2415	T2414	punct	", ",displayed
R1424	T2416	T2414	nsubj	removal,displayed
R1425	T2417	T2416	prep	of,removal
R1426	T2255	T2253	conj	sufficient,necessary
R1427	T2418	T2419	amod	individual,ligands
R1428	T2419	T2417	pobj	ligands,of
R1429	T2256	T2255	prep	for,sufficient
R143	T308	T309	poss	our,results
R1430	T2420	T2421	amod	such,as
R1431	T2421	T2419	prep	as,ligands
R1432	T2257	T2258	compound	cartilage,condensation
R1433	T2422	T2421	pobj	BMP4,as
R1434	T2423	T2424	punct	[,16
R1435	T2424	T2422	parataxis	16,BMP4
R1436	T2258	T2256	pobj	condensation,for
R1437	T2425	T2424	punct	],16
R1438	T2259	T2258	prep	in,condensation
R1439	T2426	T2422	cc	or,BMP4
R144	T309	T303	nsubj	results,indicate
R1440	T2427	T2422	conj	BMP7,BMP4
R1441	T2428	T2429	punct	[,13
R1442	T2429	T2427	parataxis	13,BMP7
R1443	T2260	T2259	pobj	chick,in
R1444	T2430	T2429	nummod	12,13
R1445	T2431	T2429	punct	",",13
R1446	T2432	T2429	punct	],13
R1447	T2261	T2262	punct	[,25
R1448	T2433	T2414	aux	has,displayed
R1449	T2434	T2414	neg	not,displayed
R145	T310	T311	mark	that,BMPs
R1450	T2262	T2234	parataxis	25,shown
R1451	T2435	T2414	dobj	defects,displayed
R1452	T2436	T2435	prep	in,defects
R1453	T2437	T2438	amod	skeletal,differentiation
R1454	T2438	T2436	pobj	differentiation,in
R1455	T2439	T2414	punct	", ",displayed
R1456	T2263	T2262	punct	],25
R1457	T2440	T2441	advmod	presumably,due
R1458	T2441	T2414	prep	due,displayed
R1459	T2442	T2441	pcomp	to,due
R146	T311	T303	ccomp	BMPs,indicate
R1460	T2264	T2234	punct	.,shown
R1461	T2443	T2444	amod	functional,redundancy
R1462	T2444	T2441	pobj	redundancy,due
R1463	T2445	T2414	punct	.,displayed
R1464	T2266	T2267	advmod	Likewise,blocks
R1465	T2447	T2448	aux	To,gain
R1466	T2448	T2449	advcl	gain,produced
R1467	T2268	T2267	punct	", ",blocks
R1468	T2450	T2451	amod	further,insights
R1469	T2451	T2448	dobj	insights,gain
R147	T312	T311	preconj	neither,BMPs
R1470	T2269	T2270	amod	conditional,knockout
R1471	T2452	T2451	prep	into,insights
R1472	T2453	T2454	det	the,roles
R1473	T2454	T2452	pobj	roles,into
R1474	T2270	T2267	nsubj	knockout,blocks
R1475	T2455	T2454	amod	potential,roles
R1476	T2456	T2454	prep	of,roles
R1477	T2457	T2458	compound	BMP,signaling
R1478	T2271	T2270	prep	of,knockout
R1479	T2458	T2456	pobj	signaling,of
R148	T313	T311	conj	act,BMPs
R1480	T2272	T2271	pobj	BmpR1A,of
R1481	T2273	T2272	cc	and,BmpR1A
R1482	T2459	T2454	prep	in,roles
R1483	T2460	T2461	compound	vertebrate,patterning
R1484	T2274	T2272	conj	BmpR1B,BmpR1A
R1485	T2461	T2459	pobj	patterning,in
R1486	T2462	T2461	compound	limb,patterning
R1487	T2275	T2276	det	all,differentiation
R1488	T2463	T2461	cc	and,patterning
R1489	T2464	T2461	conj	skeletogenesis,patterning
R149	T314	T313	prep	as,act
R1490	T2465	T2449	punct	", ",produced
R1491	T2276	T2267	dobj	differentiation,blocks
R1492	T2466	T2449	nsubj	we,produced
R1493	T2277	T2276	amod	chondrogenic,differentiation
R1494	T2467	T2449	aux	have,produced
R1495	T2468	T2449	dobj	mice,produced
R1496	T2469	T2470	advmod	simultaneously,lacking
R1497	T2278	T2267	prep	in,blocks
R1498	T2470	T2468	acl	lacking,mice
R1499	T2471	T2472	nmod	BMP2,activity
R15	T169	T167	conj	Skeletogenesis,Patterning
R150	T315	T316	amod	secondary,signals
R1500	T2472	T2470	dobj	activity,lacking
R1501	T2279	T2280	det	the,bud
R1502	T2473	T2471	cc	and,BMP2
R1503	T2474	T2471	conj	BMP4,BMP2
R1504	T2280	T2278	pobj	bud,in
R1505	T2475	T2472	cc	or,activity
R1506	T2476	T2477	nmod	BMP2,activity
R1507	T2477	T2472	conj	activity,activity
R1508	T2478	T2476	cc	and,BMP2
R1509	T2479	T2476	conj	BMP7,BMP2
R151	T316	T314	pobj	signals,as
R1510	T2480	T2472	prep	in,activity
R1511	T2281	T2280	compound	mouse,bud
R1512	T2481	T2482	det	the,mesechyme
R1513	T2482	T2480	pobj	mesechyme,in
R1514	T2483	T2482	compound	limb,mesechyme
R1515	T2282	T2280	compound	limb,bud
R1516	T2484	T2470	prep	during,lacking
R1517	T2485	T2486	compound	limb,development
R1518	T2486	T2484	pobj	development,during
R1519	T2283	T2284	punct	[,26
R152	T317	T316	advmod	downstream,signals
R1520	T2487	T2449	punct	.,produced
R1521	T2284	T2267	parataxis	26,blocks
R1522	T2489	T2490	mark	Since,are
R1523	T2285	T2284	punct	],26
R1524	T2490	T2495	advcl	are,used
R1525	T2491	T2492	nmod	Bmp2,mutants
R1526	T2286	T2267	punct	.,blocks
R1527	T2492	T2490	nsubj	mutants,are
R1528	T2493	T2491	cc	and,Bmp2
R1529	T2288	T2289	advmod	Similarly,take
R153	T318	T317	prep	of,downstream
R1530	T2494	T2491	conj	Bmp4,Bmp2
R1531	T2496	T2490	advmod	both,are
R1532	T2497	T2490	acomp	lethal,are
R1533	T2290	T2289	punct	", ",take
R1534	T2498	T2490	acomp	early,are
R1535	T2499	T2498	prep	in,early
R1536	T2500	T2499	pobj	embryogenesis,in
R1537	T2291	T2292	advmod	when,introduced
R1538	T2501	T2502	punct	[,31
R1539	T2502	T2490	parataxis	31,are
R154	T319	T320	amod	Sonic,Hedghog
R1540	T2504	T2502	punct	",",31
R1541	T2503	T2502	nummod	30,31
R1542	T2505	T2502	punct	],31
R1543	T2506	T2495	punct	", ",used
R1544	T2507	T2495	nsubj	we,used
R1545	T2508	T2509	amod	conditional,alleles
R1546	T2610	T2609	amod	broad,plate
R1547	T2611	T2609	amod	posterior,plate
R1548	T2509	T2495	dobj	alleles,used
R1549	T2612	T2609	compound	hand,plate
R155	T320	T318	pobj	Hedghog,of
R1550	T2613	T2600	punct	", ",display
R1551	T2510	T2509	prep	of,alleles
R1552	T2614	T2600	advcl	suggestive,display
R1553	T2615	T2614	prep	of,suggestive
R1554	T2511	T2512	predet	both,genes
R1555	T2616	T2617	det	a,block
R1556	T2617	T2615	pobj	block,of
R1557	T2618	T2617	prep	in,block
R1558	T2619	T2620	det	the,condensation
R1559	T2620	T2618	pobj	condensation,in
R156	T321	T320	punct	(,Hedghog
R1560	T2621	T2620	amod	chondrogenic,condensation
R1561	T2512	T2510	pobj	genes,of
R1562	T2622	T2620	prep	of,condensation
R1563	T2623	T2624	det	the,rays
R1564	T2624	T2622	pobj	rays,of
R1565	T2513	T2512	det	these,genes
R1566	T2625	T2624	amod	posterior,rays
R1567	T2626	T2624	compound	digit,rays
R1568	T2627	T2617	punct	", ",block
R1569	T2514	T2495	punct	.,used
R157	T322	T320	appos	SHH,Hedghog
R1570	T2628	T2629	advmod	rather,than
R1571	T2629	T2617	cc	than,block
R1572	T2516	T2517	det	The,alleles
R1573	T2630	T2631	det	a,change
R1574	T2631	T2617	conj	change,block
R1575	T2632	T2631	prep	in,change
R1576	T2633	T2634	amod	anterior,posterior
R1577	T2517	T2519	nsubjpass	alleles,deleted
R1578	T2634	T2636	amod	posterior,identity
R1579	T2635	T2634	punct	-,posterior
R158	T323	T316	punct	),signals
R1580	T2636	T2632	pobj	identity,in
R1581	T2518	T2517	amod	conditional,alleles
R1582	T2637	T2636	compound	digit,identity
R1583	T2638	T2600	punct	.,display
R1584	T2520	T2519	auxpass	were,deleted
R1585	T2640	T2641	prep	At,required
R1586	T2642	T2643	amod	later,stages
R1587	T2521	T2519	advmod	early,deleted
R1588	T2643	T2640	pobj	stages,At
R1589	T2644	T2641	punct	", ",required
R159	T324	T316	prep	in,signals
R1590	T2522	T2521	prep	in,early
R1591	T2645	T2646	det	the,activity
R1592	T2646	T2641	nsubjpass	activity,required
R1593	T2647	T2646	amod	combined,activity
R1594	T2523	T2524	compound	limb,development
R1595	T2648	T2646	prep	of,activity
R1596	T2649	T2648	preconj	neither,of
R1597	T2650	T2648	conj	BMP2,of
R1598	T2524	T2522	pobj	development,in
R1599	T2651	T2650	cc	and,BMP2
R16	T172	T173	nmod	Bone,protein
R160	T325	T324	pcomp	patterning,in
R1600	T2652	T2650	conj	BMP4,BMP2
R1601	T2653	T2648	cc	nor,of
R1602	T2525	T2519	prep	through,deleted
R1603	T2654	T2648	conj	of,of
R1604	T2655	T2654	pobj	BMP2,of
R1605	T2526	T2527	det	the,action
R1606	T2656	T2655	cc	and,BMP2
R1607	T2657	T2655	conj	BMP7,BMP2
R1608	T2527	T2525	pobj	action,through
R1609	T2658	T2641	auxpass	is,required
R161	T326	T327	det	the,axis
R1610	T2659	T2641	prep	for,required
R1611	T2660	T2659	pobj	chondrogenesis,for
R1612	T2661	T2641	punct	.,required
R1613	T2663	T2664	nsubjpass	Osteogenesis,initiated
R1614	T2528	T2527	prep	of,action
R1615	T2665	T2664	auxpass	is,initiated
R1616	T2666	T2664	advmod	also,initiated
R1617	T2529	T2530	det	a,transgene
R1618	T2530	T2528	pobj	transgene,of
R1619	T2667	T2664	advmod	properly,initiated
R162	T327	T325	dobj	axis,patterning
R1620	T2668	T2664	prep	in,initiated
R1621	T2669	T2670	det	the,absence
R1622	T2531	T2532	compound	cre,recombinase
R1623	T2670	T2668	pobj	absence,in
R1624	T2671	T2670	prep	of,absence
R1625	T2672	T2673	det	these,pairs
R1626	T2532	T2530	compound	recombinase,transgene
R1627	T2673	T2671	pobj	pairs,of
R1628	T2674	T2673	prep	of,pairs
R1629	T2675	T2674	pobj	factors,of
R163	T328	T327	amod	anteroposterior,axis
R1630	T2533	T2532	punct	-,recombinase
R1631	T2676	T2664	punct	.,initiated
R1632	T2534	T2530	acl	expressed,transgene
R1633	T2678	T2679	advmod	However,becomes
R1634	T2535	T2534	prep	under,expressed
R1635	T2680	T2679	punct	", ",becomes
R1636	T2681	T2679	prep	at,becomes
R1637	T2682	T2683	amod	later,stages
R1638	T2536	T2537	det	the,control
R1639	T2683	T2681	pobj	stages,at
R164	T329	T314	cc	nor,as
R1640	T2684	T2679	punct	", ",becomes
R1641	T2685	T2686	det	the,loss
R1642	T2537	T2535	pobj	control,under
R1643	T2686	T2679	nsubj	loss,becomes
R1644	T2687	T2686	amod	combined,loss
R1645	T2538	T2537	prep	of,control
R1646	T2688	T2686	prep	of,loss
R1647	T2689	T2688	pobj	BMP2,of
R1648	T2690	T2689	cc	and,BMP2
R1649	T2539	T2540	det	the,enhancer
R165	T330	T314	conj	as,as
R1650	T2691	T2689	conj	BMP4,BMP2
R1651	T2692	T2679	advmod	evidently,becomes
R1652	T2693	T2679	acomp	limiting,becomes
R1653	T2540	T2538	pobj	enhancer,of
R1654	T2694	T2679	punct	", ",becomes
R1655	T2695	T2679	cc	and,becomes
R1656	T2696	T2697	nsubj	osteogenesis,ceases
R1657	T2541	T2540	compound	Prx1,enhancer
R1658	T2697	T2679	conj	ceases,becomes
R1659	T2698	T2679	punct	.,becomes
R166	T331	T330	pobj	signals,as
R1660	T2542	T2543	punct	[,32
R1661	T2700	T2701	mark	As,result
R1662	T2701	T2714	advcl	result,is
R1663	T2702	T2703	det	all,manipulations
R1664	T2703	T2701	nsubj	manipulations,result
R1665	T2543	T2519	parataxis	32,deleted
R1666	T2704	T2703	advmod	prior,manipulations
R1667	T2705	T2703	amod	reported,manipulations
R1668	T2706	T2703	prep	of,manipulations
R1669	T2544	T2543	punct	],32
R167	T332	T331	prep	from,signals
R1670	T2707	T2708	det	the,pathway
R1671	T2708	T2706	pobj	pathway,of
R1672	T2709	T2708	compound	BMP,pathway
R1673	T2545	T2519	punct	.,deleted
R1674	T2710	T2701	prep	during,result
R1675	T2711	T2712	amod	skeletal,development
R1676	T2712	T2710	pobj	development,during
R1677	T2547	T2548	preconj	Neither,limbs
R1678	T2713	T2701	preconj	either,result
R1679	T2715	T2701	prep	in,result
R168	T333	T334	det	the,mesenchyme
R1680	T2548	T2549	nsubj	limbs,had
R1681	T2550	T2548	amod	deficient,limbs
R1682	T2716	T2717	det	a,block
R1683	T2717	T2715	pobj	block,in
R1684	T2718	T2701	prep	at,result
R1685	T2551	T2550	prep	in,deficient
R1686	T2719	T2720	det	the,stage
R1687	T2720	T2718	pobj	stage,at
R1688	T2552	T2551	pobj	BMP2,in
R1689	T2721	T2720	amod	first,stage
R169	T334	T332	pobj	mesenchyme,from
R1690	T2722	T2720	prep	of,stage
R1691	T2723	T2722	pobj	chondrogenesis,of
R1692	T2553	T2552	cc	and,BMP2
R1693	T2724	T2701	cc	or,result
R1694	T2725	T2701	conj	allow,result
R1695	T2726	T2727	amod	complete,formation
R1696	T2554	T2552	conj	BMP7,BMP2
R1697	T2727	T2725	dobj	formation,allow
R1698	T2728	T2727	amod	skeletal,formation
R1699	T2555	T2548	cc	nor,limbs
R17	T173	T175	nmod	protein,members
R170	T335	T334	amod	interdigital,mesenchyme
R1700	T2729	T2714	punct	", ",is
R1701	T2730	T2714	nsubj	this,is
R1702	T2731	T2732	det	the,demonstration
R1703	T2556	T2548	conj	those,limbs
R1704	T2732	T2714	attr	demonstration,is
R1705	T2733	T2732	amod	first,demonstration
R1706	T2734	T2732	prep	of,demonstration
R1707	T2557	T2556	amod	deficient,those
R1708	T2735	T2736	det	a,requirement
R1709	T2736	T2734	pobj	requirement,of
R171	T336	T331	prep	in,signals
R1710	T2558	T2557	prep	in,deficient
R1711	T2737	T2736	prep	for,requirement
R1712	T2738	T2739	compound	BMP,activity
R1713	T2739	T2737	pobj	activity,for
R1714	T2740	T2736	prep	in,requirement
R1715	T2741	T2742	amod	osteogenic,differentiation
R1716	T2742	T2740	pobj	differentiation,in
R1717	T2559	T2558	pobj	BMP2,in
R1718	T2743	T2714	punct	.,is
R1719	T2560	T2559	cc	and,BMP2
R172	T337	T336	pcomp	specifying,in
R1720	T2561	T2559	conj	BMP4,BMP2
R1721	T2562	T2549	dobj	defects,had
R1722	T2563	T2562	amod	suggestive,defects
R1723	T2564	T2563	prep	of,suggestive
R1724	T2565	T2564	pobj	loss,of
R1725	T2566	T2565	prep	of,loss
R1726	T2567	T2568	amod	anterior,posterior
R1727	T2568	T2570	amod	posterior,information
R1728	T2569	T2568	punct	-,posterior
R1729	T2570	T2566	pobj	information,of
R173	T338	T339	compound	digit,identity
R1730	T2571	T2570	compound	patterning,information
R1731	T2572	T2549	punct	", ",had
R1732	T2573	T2549	advcl	arguing,had
R1733	T2574	T2573	prep	against,arguing
R1734	T2575	T2576	det	these,factors
R1735	T2576	T2577	nsubj	factors,acting
R1736	T2577	T2574	pcomp	acting,against
R1737	T2578	T2577	prep	as,acting
R1738	T2579	T2580	amod	secondary,signals
R1739	T2580	T2578	pobj	signals,as
R174	T339	T337	dobj	identity,specifying
R1740	T2581	T2577	advmod	downstream,acting
R1741	T2582	T2581	prep	of,downstream
R1742	T2583	T2582	pobj	SHH,of
R1743	T2584	T2577	cc	or,acting
R1744	T2585	T2577	conj	acting,acting
R1745	T2586	T2585	prep	as,acting
R1746	T2587	T2588	amod	interdigital,determinants
R1747	T2588	T2586	pobj	determinants,as
R1748	T2589	T2588	prep	of,determinants
R1749	T2590	T2591	compound	digit,identity
R175	T340	T303	punct	.,indicate
R1750	T2591	T2589	pobj	identity,of
R1751	T2592	T2549	punct	.,had
R1752	T2594	T2595	det	The,mutants
R1753	T2595	T2600	nsubj	mutants,display
R1754	T2596	T2597	nmod	Bmp2,Bmp4
R1755	T2597	T2595	nmod	Bmp4,mutants
R1756	T2598	T2597	punct	/,Bmp4
R1757	T2599	T2595	amod	double,mutants
R1758	T2601	T2600	aux	did,display
R1759	T2602	T2603	det	a,loss
R176	T342	T343	nsubj	We,find
R1760	T2603	T2600	dobj	loss,display
R1761	T2604	T2603	prep	of,loss
R1762	T2605	T2606	amod	posterior,digits
R1763	T2606	T2604	pobj	digits,of
R1764	T2607	T2603	prep	with,loss
R1765	T2608	T2609	det	a,plate
R1766	T2609	T2607	pobj	plate,with
R1767	T2948	T2949	aux	To,investigate
R1768	T2949	T2950	advcl	investigate,constructed
R1769	T2951	T2952	det	the,roles
R177	T344	T343	aux	do,find
R1770	T2952	T2949	dobj	roles,investigate
R1771	T2953	T2952	prep	of,roles
R1772	T2954	T2955	compound	BMP,signaling
R1773	T2955	T2953	pobj	signaling,of
R1774	T2956	T2949	prep	at,investigate
R1775	T2957	T2958	amod	various,stages
R1776	T2958	T2956	pobj	stages,at
R1777	T2959	T2958	prep	of,stages
R1778	T2960	T2961	compound	limb,patterning
R1779	T2961	T2959	pobj	patterning,of
R178	T345	T346	mark	that,required
R1780	T2962	T2961	cc	and,patterning
R1781	T2963	T2961	conj	skeletogenesis,patterning
R1782	T2964	T2950	punct	", ",constructed
R1783	T2965	T2950	nsubj	we,constructed
R1784	T2966	T2967	det	a,series
R1785	T2967	T2950	dobj	series,constructed
R1786	T2968	T2967	prep	of,series
R1787	T2969	T2968	pobj	mice,of
R1788	T2970	T2969	amod	deficient,mice
R1789	T2971	T2972	advmod	singly,in
R179	T346	T343	ccomp	required,find
R1790	T2972	T2970	prep	in,deficient
R1791	T2973	T2972	cc	or,in
R1792	T2974	T2972	pobj	combination,in
R1793	T2975	T2970	prep	in,deficient
R1794	T2976	T2977	det	the,ability
R1795	T2977	T2975	pobj	ability,in
R1796	T2978	T2979	aux	to,produce
R1797	T2979	T2977	acl	produce,ability
R1798	T2980	T2979	dobj	BMP2,produce
R1799	T2981	T2980	punct	", ",BMP2
R18	T174	T173	amod	morphogenetic,protein
R180	T347	T348	det	a,level
R1800	T2982	T2980	conj	BMP4,BMP2
R1801	T2983	T2982	punct	", ",BMP4
R1802	T2984	T2982	cc	and,BMP4
R1803	T2985	T2982	conj	BMP7,BMP4
R1804	T2986	T2950	punct	.,constructed
R1805	T2988	T2989	npadvmod	BMP7,deficient
R1806	T2989	T2991	amod	deficient,mice
R1807	T2990	T2989	punct	-,deficient
R1808	T2991	T2992	nsubj	mice,survive
R1809	T2993	T2991	punct	(,mice
R181	T348	T346	nsubjpass	level,required
R1810	T2994	T2995	advmod	kindly,provided
R1811	T2995	T2991	acl	provided,mice
R1812	T2996	T2995	agent	by,provided
R1813	T2997	T2998	compound	Dr.,Robertson
R1814	T2998	T2996	pobj	Robertson,by
R1815	T2999	T2998	compound	Liz,Robertson
R1816	T3000	T2992	punct	),survive
R1817	T3001	T2992	prep	until,survive
R1818	T3002	T3001	pobj	birth,until
R1819	T3003	T2992	punct	.,survive
R182	T349	T348	compound	threshold,level
R1820	T3005	T3006	advmod	However,required
R1821	T3007	T3006	punct	", ",required
R1822	T3008	T3006	nsubjpass	BMP2,required
R1823	T3009	T3008	cc	and,BMP2
R1824	T3010	T3008	conj	BMP4,BMP2
R1825	T3011	T3006	auxpass	are,required
R1826	T3012	T3006	advmod	both,required
R1827	T3013	T3006	prep	for,required
R1828	T3014	T3013	pobj	viability,for
R1829	T3015	T3016	advmod	early,in
R183	T350	T348	prep	of,level
R1830	T3016	T3006	prep	in,required
R1831	T3017	T3018	amod	embryonic,development
R1832	T3018	T3016	pobj	development,in
R1833	T3019	T3020	punct	[,31
R1834	T3020	T3006	parataxis	31,required
R1835	T3021	T3020	nummod	30,31
R1836	T3022	T3020	punct	",",31
R1837	T3023	T3020	punct	],31
R1838	T3024	T3006	punct	.,required
R1839	T3026	T3027	nsubj	We,constructed
R184	T351	T352	compound	BMP,signaling
R1840	T3028	T3027	advmod	therefore,constructed
R1841	T3029	T3030	det	a,allele
R1842	T3030	T3027	dobj	allele,constructed
R1843	T3031	T3030	amod	conditional,allele
R1844	T3032	T3030	prep	of,allele
R1845	T3033	T3032	pobj	Bmp2,of
R1846	T3034	T3027	punct	", ",constructed
R1847	T3035	T3027	advcl	introducing,constructed
R1848	T3036	T3037	compound	loxP,sites
R1849	T3037	T3035	dobj	sites,introducing
R185	T352	T350	pobj	signaling,of
R1850	T3038	T3037	acl	flanking,sites
R1851	T3039	T3038	dobj	exon,flanking
R1852	T3040	T3039	nummod	3,exon
R1853	T3041	T3027	punct	.,constructed
R1854	T3043	T3044	nsubj	We,obtained
R1855	T3045	T3046	det	a,allele
R1856	T3046	T3044	dobj	allele,obtained
R1857	T3047	T3046	amod	conditional,allele
R1858	T3048	T3046	prep	of,allele
R1859	T3049	T3048	pobj	Bmp4,of
R186	T353	T346	auxpass	is,required
R1860	T3050	T3046	punct	", ",allele
R1861	T3051	T3052	prep	in,flanked
R1862	T3052	T3046	relcl	flanked,allele
R1863	T3053	T3051	pobj	which,in
R1864	T3054	T3052	nsubjpass	exon,flanked
R1865	T3055	T3054	nummod	4,exon
R1866	T3056	T3052	auxpass	is,flanked
R1867	T3057	T3052	prep	by,flanked
R1868	T3058	T3059	compound	loxP,sites
R1869	T3059	T3057	pobj	sites,by
R187	T354	T346	prep	for,required
R1870	T3060	T3044	punct	", ",obtained
R1871	T3061	T3044	prep	from,obtained
R1872	T3062	T3063	compound	Dr.,Kulessa
R1873	T3063	T3061	pobj	Kulessa,from
R1874	T3064	T3063	compound	Holger,Kulessa
R1875	T3065	T3063	cc	and,Kulessa
R1876	T3066	T3067	compound	Dr.,Hogan
R1877	T3067	T3063	conj	Hogan,Kulessa
R1878	T3068	T3067	compound	Brigid,Hogan
R1879	T3069	T3070	punct	[,16
R188	T355	T356	det	the,onset
R1880	T3070	T3044	parataxis	16,obtained
R1881	T3071	T3070	punct	],16
R1882	T3072	T3044	punct	.,obtained
R1883	T3074	T3075	nsubjpass	Both,expected
R1884	T3076	T3074	prep	of,Both
R1885	T3077	T3078	det	these,alleles
R1886	T3078	T3076	pobj	alleles,of
R1887	T3079	T3075	aux	would,expected
R1888	T3080	T3075	auxpass	be,expected
R1889	T3081	T3082	aux	to,result
R189	T356	T354	pobj	onset,for
R1890	T3082	T3075	xcomp	result,expected
R1891	T3083	T3082	prep	in,result
R1892	T3084	T3085	amod	null,alleles
R1893	T3085	T3083	pobj	alleles,in
R1894	T3086	T3082	prep	following,result
R1895	T3087	T3086	pobj	recombination,following
R1896	T3088	T3089	punct	(,see
R1897	T3089	T3075	parataxis	see,expected
R1898	T3090	T3089	dobj	Materials,see
R1899	T3091	T3090	cc	and,Materials
R19	T175	T180	nsubjpass	members,expressed
R190	T357	T356	prep	of,onset
R1900	T3092	T3090	conj	Methods,Materials
R1901	T3093	T3089	prep	for,see
R1902	T3094	T3093	pobj	details,for
R1903	T3095	T3089	punct	),see
R1904	T3096	T3075	punct	.,expected
R1905	T3098	T3099	aux	To,inactivate
R1906	T3099	T3101	advcl	inactivate,used
R1907	T3100	T3099	advmod	conditionally,inactivate
R1908	T3102	T3099	dobj	Bmp2,inactivate
R1909	T3103	T3102	cc	and,Bmp2
R191	T358	T357	pobj	chondrogenesis,of
R1910	T3104	T3102	conj	Bmp4,Bmp2
R1911	T3105	T3099	prep	in,inactivate
R1912	T3106	T3107	det	the,limb
R1913	T3107	T3105	pobj	limb,in
R1914	T3108	T3101	punct	", ",used
R1915	T3109	T3101	nsubj	we,used
R1916	T3110	T3111	det	a,transgene
R1917	T3111	T3101	dobj	transgene,used
R1918	T3112	T3113	advmod	well,characterized
R1919	T3113	T3111	amod	characterized,transgene
R192	T359	T343	punct	", ",find
R1920	T3114	T3113	punct	-,characterized
R1921	T3115	T3116	prep	in,expressed
R1922	T3116	T3111	advcl	expressed,transgene
R1923	T3117	T3115	pobj	which,in
R1924	T3118	T3119	compound	cre,recombinase
R1925	T3119	T3116	nsubjpass	recombinase,expressed
R1926	T3120	T3119	punct	-,recombinase
R1927	T3121	T3116	auxpass	is,expressed
R1928	T3122	T3116	prep	under,expressed
R1929	T3123	T3124	det	the,control
R193	T360	T343	cc	and,find
R1930	T3124	T3122	pobj	control,under
R1931	T3125	T3124	prep	of,control
R1932	T3126	T3127	det	the,enhancer
R1933	T3127	T3125	pobj	enhancer,of
R1934	T3128	T3127	compound	Prx1,enhancer
R1935	T3129	T3127	compound	limb,enhancer
R1936	T3130	T3131	punct	[,32
R1937	T3131	T3101	parataxis	32,used
R1938	T3132	T3131	punct	],32
R1939	T3133	T3101	punct	.,used
R194	T361	T362	advmod	hence,fail
R1940	T3135	T3136	det	This,transgene
R1941	T3136	T3137	nsubj	transgene,expresses
R1942	T3138	T3137	dobj	cre,expresses
R1943	T3139	T3140	advmod	very,early
R1944	T3140	T3137	advmod	early,expresses
R1945	T3141	T3140	prep	in,early
R1946	T3142	T3143	compound	limb,development
R1947	T3143	T3141	pobj	development,in
R1948	T3144	T3137	punct	", ",expresses
R1949	T3145	T3137	advcl	resulting,expresses
R195	T362	T343	conj	fail,find
R1950	T3146	T3145	prep	in,resulting
R1951	T3147	T3148	amod	complete,recombination
R1952	T3148	T3146	pobj	recombination,in
R1953	T3149	T3148	prep	of,recombination
R1954	T3150	T3151	amod	floxed,alleles
R1955	T3151	T3149	pobj	alleles,of
R1956	T3152	T3145	prep	at,resulting
R1957	T3153	T3154	amod	early,stages
R1958	T3154	T3152	pobj	stages,at
R1959	T3155	T3154	compound	limb,stages
R196	T363	T364	det	some,condensations
R1960	T3156	T3154	compound	bud,stages
R1961	T3157	T3137	punct	.,expresses
R1962	T3159	T3160	nsubj	We,verified
R1963	T3161	T3162	det	the,ability
R1964	T3162	T3160	dobj	ability,verified
R1965	T3163	T3162	prep	of,ability
R1966	T3164	T3165	compound	Prx1,cre
R1967	T3165	T3163	pobj	cre,of
R1968	T3166	T3165	punct	:,cre
R1969	T3167	T3165	punct	:,cre
R197	T364	T362	nsubj	condensations,fail
R1970	T3168	T3169	aux	to,recombine
R1971	T3169	T3162	acl	recombine,ability
R1972	T3170	T3171	det	the,alleles
R1973	T3171	T3169	dobj	alleles,recombine
R1974	T3172	T3171	amod	conditional,alleles
R1975	T3173	T3171	nmod	Bmp2,alleles
R1976	T3174	T3173	cc	and,Bmp2
R1977	T3175	T3173	conj	Bmp4,Bmp2
R1978	T3176	T3169	prep	at,recombine
R1979	T3177	T3178	amod	earliest,stages
R198	T365	T364	amod	chondrogenic,condensations
R1980	T3178	T3176	pobj	stages,at
R1981	T3179	T3180	compound	limb,bud
R1982	T3180	T3178	compound	bud,stages
R1983	T3181	T3160	advcl	using,verified
R1984	T3182	T3183	advmod	in,situ
R1985	T3183	T3184	amod	situ,hybridization
R1986	T3184	T3181	dobj	hybridization,using
R1987	T3185	T3160	punct	.,verified
R1988	T3187	T3188	nsubjpass	Bmp2,expressed
R1989	T3189	T3188	auxpass	is,expressed
R199	T366	T367	aux	to,form
R1990	T3190	T3188	advmod	first,expressed
R1991	T3191	T3188	prep	in,expressed
R1992	T3192	T3193	det	the,mesenchyme
R1993	T3193	T3191	pobj	mesenchyme,in
R1994	T3194	T3193	compound	limb,mesenchyme
R1995	T3195	T3188	prep	at,expressed
R1996	T3196	T3197	amod	embryonic,day
R1997	T3197	T3195	pobj	day,at
R1998	T3198	T3197	punct	(,day
R1999	T3199	T3197	appos	E,day
R2	T156	T157	det	the,Roles
R20	T176	T173	punct	(,protein
R200	T367	T362	xcomp	form,fail
R2000	T3200	T3197	punct	),day
R2001	T3201	T3197	nummod	10.5,day
R2002	T3202	T3188	prep	in,expressed
R2003	T3203	T3204	det	the,mouse
R2004	T3204	T3202	pobj	mouse,in
R2005	T3205	T3206	punct	(,asterisk
R2006	T3206	T3188	parataxis	asterisk,expressed
R2007	T3207	T3208	compound	Figure,1A
R2008	T3208	T3206	dep	1A,asterisk
R2009	T3209	T3206	punct	", ",asterisk
R201	T368	T367	prep	in,form
R2010	T3210	T3206	punct	),asterisk
R2011	T3211	T3188	punct	.,expressed
R2012	T3213	T3214	prep	By,appears
R2013	T3215	T3216	det	the,time
R2014	T3216	T3213	pobj	time,By
R2015	T3217	T3218	poss	its,expression
R2016	T3218	T3219	nsubj	expression,is
R2017	T3219	T3216	advcl	is,time
R2018	T3220	T3219	advmod	first,is
R2019	T3221	T3219	acomp	detectable,is
R202	T369	T368	pobj	limbs,in
R2020	T3222	T3214	punct	", ",appears
R2021	T3223	T3224	det	the,allele
R2022	T3224	T3214	nsubj	allele,appears
R2023	T3225	T3224	amod	floxed,allele
R2024	T3226	T3224	compound	Bmp2,allele
R2025	T3227	T3228	aux	to,recombined
R2026	T3228	T3214	xcomp	recombined,appears
R2027	T3229	T3228	auxpass	be,recombined
R2028	T3230	T3228	advmod	completely,recombined
R2029	T3231	T3228	prep	in,recombined
R203	T370	T369	amod	deficient,limbs
R2030	T3232	T3233	det	the,mesenchyme
R2031	T3233	T3231	pobj	mesenchyme,in
R2032	T3234	T3233	compound	limb,mesenchyme
R2033	T3235	T3214	punct	", ",appears
R2034	T3236	T3237	mark	as,detect
R2035	T3237	T3214	advcl	detect,appears
R2036	T3238	T3239	amod	whole,mount
R2037	T3239	T3240	nmod	mount,hybridization
R2038	T3240	T3237	nsubj	hybridization,detect
R2039	T3241	T3242	advmod	in,situ
R204	T371	T370	prep	in,deficient
R2040	T3242	T3240	amod	situ,hybridization
R2041	T3243	T3237	aux	does,detect
R2042	T3244	T3237	neg	not,detect
R2043	T3245	T3246	det	any,transcription
R2044	T3246	T3237	dobj	transcription,detect
R2045	T3247	T3246	amod	mesenchymal,transcription
R2046	T3248	T3246	compound	Bmp2,transcription
R2047	T3249	T3250	punct	(,1B
R2048	T3250	T3214	parataxis	1B,appears
R2049	T3251	T3250	compound	Figure,1B
R205	T372	T373	preconj	both,BMP2
R2050	T3252	T3250	punct	),1B
R2051	T3253	T3214	punct	.,appears
R2052	T3255	T3256	prep	At,expressed
R2053	T3257	T3255	pobj	E10.5,At
R2054	T3258	T3256	nsubjpass	Bmp4,expressed
R2055	T3259	T3256	auxpass	is,expressed
R2056	T3260	T3256	prep	in,expressed
R2057	T3261	T3262	det	the,mesenchyme
R2058	T3262	T3260	pobj	mesenchyme,in
R2059	T3263	T3262	compound	mouse,mesenchyme
R206	T373	T371	pobj	BMP2,in
R2060	T3264	T3262	compound	limb,mesenchyme
R2061	T3265	T3256	prep	in,expressed
R2062	T3266	T3267	nummod	two,stripes
R2063	T3267	T3265	pobj	stripes,in
R2064	T3268	T3267	prep	at,stripes
R2065	T3269	T3270	det	the,margins
R2066	T3270	T3268	pobj	margins,at
R2067	T3271	T3270	amod	anterior,margins
R2068	T3272	T3271	cc	and,anterior
R2069	T3273	T3271	conj	posterior,anterior
R207	T374	T373	cc	and,BMP2
R2070	T3274	T3275	punct	(,arrows
R2071	T3275	T3256	parataxis	arrows,expressed
R2072	T3276	T3277	compound	Figure,1C
R2073	T3277	T3275	dep	1C,arrows
R2074	T3278	T3275	punct	", ",arrows
R2075	T3279	T3275	amod	red,arrows
R2076	T3280	T3275	punct	),arrows
R2077	T3281	T3256	punct	.,expressed
R2078	T3283	T3284	det	These,domains
R2079	T3284	T3286	nsubjpass	domains,lost
R208	T375	T373	conj	BMP4,BMP2
R2080	T3285	T3284	compound	expression,domains
R2081	T3287	T3286	auxpass	are,lost
R2082	T3288	T3286	advmod	completely,lost
R2083	T3289	T3286	prep	by,lost
R2084	T3290	T3289	pobj	E10.5,by
R2085	T3291	T3286	prep	in,lost
R2086	T3292	T3293	det	the,presence
R2087	T3293	T3291	pobj	presence,in
R2088	T3294	T3293	prep	of,presence
R2089	T3295	T3296	det	the,transgene
R209	T376	T362	punct	.,fail
R2090	T3296	T3294	pobj	transgene,of
R2091	T3297	T3298	compound	Prx1,cre
R2092	T3298	T3296	compound	cre,transgene
R2093	T3299	T3298	punct	:,cre
R2094	T3300	T3298	punct	:,cre
R2095	T3301	T3302	punct	(,1D
R2096	T3302	T3286	parataxis	1D,lost
R2097	T3303	T3302	compound	Figure,1D
R2098	T3304	T3302	punct	),1D
R2099	T3305	T3286	punct	.,lost
R21	T177	T173	appos	BMP,protein
R210	T378	T379	advmod	However,proceeds
R2100	T3307	T3308	nsubjpass	Bmp2,expressed
R2101	T3309	T3307	cc	and,Bmp2
R2102	T3310	T3307	conj	Bmp4,Bmp2
R2103	T3311	T3308	auxpass	are,expressed
R2104	T3312	T3308	advmod	also,expressed
R2105	T3313	T3308	prep	in,expressed
R2106	T3314	T3315	det	the,AER
R2107	T3315	T3313	pobj	AER,in
R2108	T3316	T3315	punct	", ",AER
R2109	T3317	T3318	advmod	where,is
R211	T380	T379	punct	", ",proceeds
R2110	T3318	T3315	relcl	is,AER
R2111	T3319	T3320	compound	Prx1,cre
R2112	T3320	T3318	nsubj	cre,is
R2113	T3321	T3320	punct	:,cre
R2114	T3322	T3320	punct	:,cre
R2115	T3323	T3318	acomp	inactive,is
R2116	T3324	T3308	punct	", ",expressed
R2117	T3325	T3308	cc	and,expressed
R2118	T3326	T3327	det	these,domains
R2119	T3327	T3328	nsubjpass	domains,affected
R212	T381	T379	prep	in,proceeds
R2120	T3328	T3308	conj	affected,expressed
R2121	T3329	T3327	prep	of,domains
R2122	T3330	T3329	pobj	expression,of
R2123	T3331	T3328	auxpass	are,affected
R2124	T3332	T3328	neg	not,affected
R2125	T3333	T3334	punct	(,arrows
R2126	T3334	T3328	parataxis	arrows,affected
R2127	T3335	T3336	compound	Figure,1A
R2128	T3336	T3334	dep	1A,arrows
R2129	T3337	T3338	punct	–,1D
R213	T382	T383	det	the,condensations
R2130	T3338	T3336	prep	1D,1A
R2131	T3339	T3334	punct	", ",arrows
R2132	T3340	T3334	amod	black,arrows
R2133	T3341	T3334	punct	),arrows
R2134	T3342	T3328	punct	.,affected
R2135	T3720	T3721	det	An,Series
R2136	T3722	T3723	amod	Allelic,Nonallelic
R2137	T3723	T3721	amod	Nonallelic,Series
R2138	T3724	T3723	punct	/,Nonallelic
R2139	T3725	T3721	prep	of,Series
R214	T383	T381	pobj	condensations,in
R2140	T3726	T3727	npadvmod	BMP,Deficient
R2141	T3727	T3729	amod	Deficient,Limbs
R2142	T3728	T3727	punct	-,Deficient
R2143	T3729	T3725	pobj	Limbs,of
R2144	T3731	T3732	nsubjpass	Mice,generated
R2145	T3733	T3732	auxpass	were,generated
R2146	T3734	T3732	prep	with,generated
R2147	T3735	T3734	pobj	limbs,with
R2148	T3736	T3735	amod	deficient,limbs
R2149	T3737	T3736	prep	in,deficient
R215	T384	T385	dep	that,form
R2150	T3738	T3737	pobj	BMP2,in
R2151	T3739	T3738	punct	", ",BMP2
R2152	T3740	T3738	conj	BMP4,BMP2
R2153	T3741	T3740	punct	", ",BMP4
R2154	T3742	T3740	cc	or,BMP4
R2155	T3743	T3740	conj	BMP7,BMP4
R2156	T3744	T3738	punct	", ",BMP2
R2157	T3745	T3746	preconj	both,BMP2
R2158	T3746	T3738	conj	BMP2,BMP2
R2159	T3747	T3746	cc	and,BMP2
R216	T385	T383	relcl	form,condensations
R2160	T3748	T3746	conj	BMP4,BMP2
R2161	T3749	T3746	punct	", ",BMP2
R2162	T3750	T3746	cc	or,BMP2
R2163	T3751	T3752	preconj	both,BMP2
R2164	T3752	T3746	conj	BMP2,BMP2
R2165	T3753	T3752	cc	and,BMP2
R2166	T3754	T3752	conj	BMP7,BMP2
R2167	T3755	T3732	punct	.,generated
R2168	T3757	T3758	aux	To,obtain
R2169	T3758	T3759	advcl	obtain,examined
R217	T386	T385	aux	do,form
R2170	T3760	T3761	det	an,indication
R2171	T3761	T3758	dobj	indication,obtain
R2172	T3762	T3761	amod	initial,indication
R2173	T3763	T3761	prep	of,indication
R2174	T3764	T3765	det	the,range
R2175	T3765	T3763	pobj	range,of
R2176	T3766	T3765	prep	of,range
R2177	T3767	T3766	pobj	phenotypes,of
R2178	T3768	T3767	acl	produced,phenotypes
R2179	T3769	T3768	prep	in,produced
R218	T387	T379	punct	", ",proceeds
R2180	T3770	T3771	det	these,animals
R2181	T3771	T3769	pobj	animals,in
R2182	T3772	T3759	punct	", ",examined
R2183	T3773	T3759	nsubj	we,examined
R2184	T3774	T3759	advmod	first,examined
R2185	T3775	T3776	det	the,skeletons
R2186	T3776	T3759	dobj	skeletons,examined
R2187	T3777	T3776	compound	limb,skeletons
R2188	T3778	T3776	prep	of,skeletons
R2189	T3779	T3780	amod	newborn,animals
R219	T388	T389	amod	subsequent,differentiation
R2190	T3780	T3778	pobj	animals,of
R2191	T3781	T3759	punct	.,examined
R2192	T3783	T3784	npadvmod	BMP2,deficient
R2193	T3784	T3786	amod	deficient,limbs
R2194	T3785	T3784	punct	-,deficient
R2195	T3786	T3787	nsubj	limbs,appeared
R2196	T3788	T3789	advmod	remarkably,normal
R2197	T3789	T3787	oprd	normal,appeared
R2198	T3790	T3787	punct	", ",appeared
R2199	T3791	T3792	preconj	both,in
R22	T178	T175	punct	),members
R220	T389	T379	nsubj	differentiation,proceeds
R2200	T3792	T3787	prep	in,appeared
R2201	T3793	T3794	amod	skeletal,pattern
R2202	T3794	T3792	pobj	pattern,in
R2203	T3795	T3792	cc	and,in
R2204	T3796	T3792	conj	in,in
R2205	T3797	T3798	amod	gross,aspects
R2206	T3798	T3796	pobj	aspects,in
R2207	T3799	T3798	prep	of,aspects
R2208	T3800	T3799	pobj	chondrogenesis,of
R2209	T3801	T3800	cc	and,chondrogenesis
R221	T390	T389	amod	chondrogenic,differentiation
R2210	T3802	T3800	conj	osteogenesis,chondrogenesis
R2211	T3803	T3798	acl	indicated,aspects
R2212	T3804	T3803	prep	by,indicated
R2213	T3805	T3806	amod	Alcian,blue
R2214	T3806	T3807	amod	blue,red
R2215	T3807	T3810	amod	red,staining
R2216	T3808	T3807	punct	/,red
R2217	T3809	T3807	amod	Alizarin,red
R2218	T3810	T3804	pobj	staining,by
R2219	T3811	T3787	punct	.,appeared
R222	T391	T379	advmod	normally,proceeds
R2220	T3813	T3814	det	The,defect
R2221	T3814	T3817	nsubj	defect,is
R2222	T3815	T3814	nummod	one,defect
R2223	T3816	T3814	amod	notable,defect
R2224	T3818	T3814	prep	in,defect
R2225	T3819	T3820	det	the,skeleton
R2226	T3820	T3818	pobj	skeleton,in
R2227	T3821	T3820	amod	appendicular,skeleton
R2228	T3822	T3820	prep	of,skeleton
R2229	T3823	T3824	det	these,animals
R223	T392	T393	advmod	even,in
R2230	T3824	T3822	pobj	animals,of
R2231	T3825	T3826	det	a,malformation
R2232	T3826	T3817	attr	malformation,is
R2233	T3827	T3826	amod	characteristic,malformation
R2234	T3828	T3826	prep	of,malformation
R2235	T3829	T3830	det	the,scapula
R2236	T3830	T3828	pobj	scapula,of
R2237	T3831	T3832	punct	(,arrow
R2238	T3832	T3817	parataxis	arrow,is
R2239	T3833	T3832	dep	Figure,arrow
R224	T393	T379	prep	in,proceeds
R2240	T3834	T3833	nummod	1,Figure
R2241	T3835	T3832	punct	", ",arrow
R2242	T3836	T3832	amod	red,arrow
R2243	T3837	T3832	punct	),arrow
R2244	T3838	T3817	punct	.,is
R2245	T3840	T3841	npadvmod	BMP2,deficient
R2246	T3841	T3843	amod	deficient,limbs
R2247	T3842	T3841	punct	-,deficient
R2248	T3843	T3844	nsubj	limbs,exhibit
R2249	T3845	T3844	advmod	also,exhibit
R225	T394	T395	det	the,absence
R2250	T3846	T3847	compound	3,4
R2251	T3847	T3849	nummod	4,syndactyly
R2252	T3848	T3847	punct	/,4
R2253	T3849	T3844	dobj	syndactyly,exhibit
R2254	T3850	T3851	amod	soft,tissue
R2255	T3851	T3849	compound	tissue,syndactyly
R2256	T3852	T3851	punct	-,tissue
R2257	T3853	T3849	prep	with,syndactyly
R2258	T3854	T3855	amod	variable,penetrance
R2259	T3855	T3853	pobj	penetrance,with
R226	T395	T393	pobj	absence,in
R2260	T3856	T3857	punct	(,Figure
R2261	T3857	T3844	parataxis	Figure,exhibit
R2262	T3858	T3857	nummod	2,Figure
R2263	T3859	T3857	punct	),Figure
R2264	T3860	T3844	punct	.,exhibit
R2265	T3862	T3863	nsubjpass	Mice,described
R2266	T3864	T3862	prep	with,Mice
R2267	T3865	T3864	pobj	limbs,with
R2268	T3866	T3865	amod	deficient,limbs
R2269	T3867	T3866	prep	in,deficient
R227	T396	T395	prep	of,absence
R2270	T3868	T3869	compound	BMP4,activity
R2271	T3869	T3867	pobj	activity,in
R2272	T3870	T3865	cc	and,limbs
R2273	T3871	T3872	det	the,limbs
R2274	T3872	T3865	conj	limbs,limbs
R2275	T3873	T3872	prep	of,limbs
R2276	T3874	T3875	compound	Bmp7,mutant
R2277	T3875	T3876	compound	mutant,mice
R2278	T3876	T3873	pobj	mice,of
R2279	T3877	T3863	aux	have,described
R228	T397	T396	pobj	BMP2,of
R2280	T3878	T3863	advmod	both,described
R2281	T3879	T3863	auxpass	been,described
R2282	T3880	T3863	advmod	previously,described
R2283	T3881	T3863	punct	.,described
R2284	T3883	T3884	mark	As,reported
R2285	T3884	T3885	advcl	reported,display
R2286	T3886	T3887	punct	[,16
R2287	T3887	T3884	parataxis	16,reported
R2288	T3888	T3887	punct	],16
R2289	T3889	T3885	punct	", ",display
R229	T398	T397	cc	and,BMP2
R2290	T3890	T3885	prep	in,display
R2291	T3891	T3892	det	the,absence
R2292	T3892	T3890	pobj	absence,in
R2293	T3893	T3892	prep	of,absence
R2294	T3894	T3895	compound	BMP4,activity
R2295	T3895	T3893	pobj	activity,of
R2296	T3896	T3885	punct	", ",display
R2297	T3897	T3885	nsubj	limbs,display
R2298	T3898	T3899	det	a,penetrance
R2299	T3899	T3885	dobj	penetrance,display
R23	T179	T175	compound	family,members
R230	T399	T397	conj	BMP7,BMP2
R2300	T3900	T3899	amod	variable,penetrance
R2301	T3901	T3899	prep	of,penetrance
R2302	T3902	T3903	amod	preaxial,polydactyly
R2303	T3903	T3901	pobj	polydactyly,of
R2304	T3904	T3902	cc	and,preaxial
R2305	T3905	T3902	conj	postaxial,preaxial
R2306	T3906	T3885	punct	", ",display
R2307	T3907	T3885	cc	but,display
R2308	T3908	T3909	advmod	otherwise,take
R2309	T3909	T3885	conj	take,display
R231	T400	T397	cc	or,BMP2
R2310	T3910	T3909	punct	", ",take
R2311	T3911	T3912	amod	normal,patterns
R2312	T3912	T3909	nsubj	patterns,take
R2313	T3913	T3912	compound	digit,patterns
R2314	T3914	T3912	cc	and,patterns
R2315	T3915	T3916	advmod	apparently,normal
R2316	T3916	T3917	amod	normal,differentiation
R2317	T3917	T3912	conj	differentiation,patterns
R2318	T3918	T3917	amod	skeletal,differentiation
R2319	T3919	T3920	punct	(,1G
R232	T401	T397	conj	BMP2,BMP2
R2320	T3920	T3917	parataxis	1G,differentiation
R2321	T3921	T3920	compound	Figure,1G
R2322	T3922	T3920	cc	and,1G
R2323	T3923	T3920	conj	1O,1G
R2324	T3924	T3920	punct	),1G
R2325	T3925	T3909	dobj	place,take
R2326	T3926	T3909	punct	.,take
R2327	T3928	T3929	mark	As,described
R2328	T3929	T3931	advcl	described,have
R2329	T3930	T3929	advmod	previously,described
R233	T402	T401	cc	and,BMP2
R2330	T3932	T3931	punct	", ",have
R2331	T3933	T3931	nsubj	mice,have
R2332	T3934	T3933	amod	homozygous,mice
R2333	T3935	T3934	prep	for,homozygous
R2334	T3936	T3937	det	a,mutation
R2335	T3937	T3935	pobj	mutation,for
R2336	T3938	T3937	amod	null,mutation
R2337	T3939	T3937	prep	in,mutation
R2338	T3940	T3939	pobj	Bmp7,in
R2339	T3941	T3942	punct	[,12
R234	T403	T401	conj	BMP4,BMP2
R2340	T3942	T3933	parataxis	12,mice
R2341	T3943	T3942	punct	],12
R2342	T3944	T3945	det	no,defects
R2343	T3945	T3931	dobj	defects,have
R2344	T3946	T3931	prep	in,have
R2345	T3947	T3948	det	the,formation
R2346	T3948	T3946	pobj	formation,in
R2347	T3949	T3948	prep	of,formation
R2348	T3950	T3951	det	the,elements
R2349	T3951	T3949	pobj	elements,of
R235	T404	T379	punct	.,proceeds
R2350	T3952	T3951	amod	normal,elements
R2351	T3953	T3951	amod	appendicular,elements
R2352	T3954	T3951	amod	skeletal,elements
R2353	T3955	T3956	punct	(,1H
R2354	T3956	T3931	parataxis	1H,have
R2355	T3957	T3956	compound	Figure,1H
R2356	T3958	T3956	cc	and,1H
R2357	T3959	T3956	conj	1P,1H
R2358	T3960	T3956	punct	),1H
R2359	T3961	T3931	punct	.,have
R236	T406	T407	prep	In,find
R2360	T3963	T3964	nsubj	We,observe
R2361	T3965	T3964	aux	do,observe
R2362	T3966	T3964	advmod	occasionally,observe
R2363	T3967	T3968	amod	preaxial,polydactyly
R2364	T3968	T3964	dobj	polydactyly,observe
R2365	T3969	T3964	prep	in,observe
R2366	T3970	T3971	det	these,mutants
R2367	T3971	T3969	pobj	mutants,in
R2368	T3972	T3964	punct	.,observe
R2369	T3974	T3975	nmod	Compound,mice
R237	T408	T406	pobj	contrast,In
R2370	T3975	T3977	nsubj	mice,show
R2371	T3976	T3975	amod	heterozygous,mice
R2372	T3978	T3975	punct	", ",mice
R2373	T3979	T3975	prep	with,mice
R2374	T3980	T3981	nummod	one,copy
R2375	T3981	T3979	pobj	copy,with
R2376	T3982	T3981	amod	functional,copy
R2377	T3983	T3981	appos	each,copy
R2378	T3984	T3981	prep	of,copy
R2379	T3985	T3984	pobj	Bmp2,of
R238	T409	T407	punct	", ",find
R2380	T3986	T3985	cc	and,Bmp2
R2381	T3987	T3985	conj	Bmp4,Bmp2
R2382	T3988	T3981	prep	in,copy
R2383	T3989	T3990	det	the,limb
R2384	T3990	T3988	pobj	limb,in
R2385	T3991	T3992	punct	(,C
R2386	T3992	T3981	parataxis	C,copy
R2387	T3993	T3992	nmod	Bmp2,C
R2388	T3994	T3992	punct	+,C
R2389	T3995	T3992	punct	/,C
R239	T410	T407	nsubj	we,find
R2390	T3996	T3992	punct	;,C
R2391	T3997	T3998	nmod	Bmp4,C
R2392	T3998	T3992	appos	C,C
R2393	T3999	T3998	punct	+,C
R2394	T4000	T3998	punct	/,C
R2395	T4001	T3992	punct	;,C
R2396	T4002	T4003	compound	Prx1,cre
R2397	T4003	T3992	appos	cre,C
R2398	T4004	T4003	punct	:,cre
R2399	T4005	T4003	punct	:,cre
R24	T181	T175	punct	", ",members
R240	T411	T412	mark	that,results
R2400	T4006	T3992	punct	),C
R2401	T4007	T3977	punct	", ",show
R2402	T4008	T4009	det	no,effect
R2403	T4009	T3977	dobj	effect,show
R2404	T4010	T4009	prep	on,effect
R2405	T4011	T4012	preconj	either,patterning
R2406	T4012	T4010	pobj	patterning,on
R2407	T4013	T4012	compound	limb,patterning
R2408	T4014	T4012	cc	or,patterning
R2409	T4015	T4012	conj	skeletogenesis,patterning
R241	T412	T407	ccomp	results,find
R2410	T4016	T4017	punct	(,data
R2411	T4017	T3977	meta	data,show
R2412	T4018	T4017	amod	unpublished,data
R2413	T4019	T4017	punct	),data
R2414	T4020	T3977	punct	.,show
R2415	T4022	T4023	advmod	Similarly,are
R2416	T4024	T4023	punct	", ",are
R2417	T4025	T4026	compound	limb,skeletons
R2418	T4026	T4023	nsubj	skeletons,are
R2419	T4027	T4026	prep	of,skeletons
R242	T413	T414	det	the,loss
R2420	T4028	T4027	pobj	mice,of
R2421	T4029	T4030	prep	in,removed
R2422	T4030	T4028	relcl	removed,mice
R2423	T4031	T4029	pobj	which,in
R2424	T4032	T4033	det	both,copies
R2425	T4033	T4030	nsubjpass	copies,removed
R2426	T4034	T4033	prep	of,copies
R2427	T4035	T4036	det	the,gene
R2428	T4036	T4034	pobj	gene,of
R2429	T4037	T4036	compound	Bmp4,gene
R243	T414	T412	nsubj	loss,results
R2430	T4038	T4033	cc	and,copies
R2431	T4039	T4040	nummod	one,copy
R2432	T4040	T4033	conj	copy,copies
R2433	T4041	T4040	prep	of,copy
R2434	T4042	T4043	det	the,gene
R2435	T4043	T4041	pobj	gene,of
R2436	T4044	T4043	compound	Bmp2,gene
R2437	T4045	T4030	aux	have,removed
R2438	T4046	T4030	auxpass	been,removed
R2439	T4047	T4030	advmod	conditionally,removed
R244	T415	T414	prep	of,loss
R2440	T4048	T4030	prep	in,removed
R2441	T4049	T4050	det	the,limb
R2442	T4050	T4048	pobj	limb,in
R2443	T4051	T4028	punct	(,mice
R2444	T4052	T4053	nmod	Bmp2,C
R2445	T4053	T4028	appos	C,mice
R2446	T4054	T4053	punct	+,C
R2447	T4055	T4053	punct	/,C
R2448	T4056	T4053	punct	;,C
R2449	T4057	T4058	compound	Bmp4C,C
R245	T416	T417	preconj	both,BMP2
R2450	T4058	T4053	appos	C,C
R2451	T4059	T4058	punct	/,C
R2452	T4060	T4053	punct	;,C
R2453	T4061	T4062	compound	Prx1,cre
R2454	T4062	T4053	appos	cre,C
R2455	T4063	T4062	punct	:,cre
R2456	T4064	T4062	punct	:,cre
R2457	T4065	T4023	punct	),are
R2458	T4066	T4067	advmod	phenotypically,normal
R2459	T4067	T4023	acomp	normal,are
R246	T417	T415	pobj	BMP2,of
R2460	T4068	T4023	advmod	other,are
R2461	T4069	T4068	prep	than,other
R2462	T4070	T4069	pcomp	exhibiting,than
R2463	T4071	T4072	det	the,polydactyly
R2464	T4072	T4070	dobj	polydactyly,exhibiting
R2465	T4073	T4072	amod	variable,polydactyly
R2466	T4074	T4072	nmod	penetrance,polydactyly
R2467	T4075	T4072	amod	preaxial,polydactyly
R2468	T4076	T4075	cc	and,preaxial
R2469	T4077	T4075	conj	postaxial,preaxial
R247	T418	T417	cc	and,BMP2
R2470	T4078	T4072	acl	seen,polydactyly
R2471	T4079	T4078	prep	in,seen
R2472	T4080	T4079	pobj	mice,in
R2473	T4081	T4080	amod	deficient,mice
R2474	T4082	T4081	prep	in,deficient
R2475	T4083	T4084	compound	BMP4,activity
R2476	T4084	T4082	pobj	activity,in
R2477	T4085	T4084	advmod	alone,activity
R2478	T4086	T4087	punct	(,1J
R2479	T4087	T4023	parataxis	1J,are
R248	T419	T417	conj	BMP4,BMP2
R2480	T4088	T4087	compound	Figure,1J
R2481	T4089	T4087	cc	and,1J
R2482	T4090	T4087	conj	1R,1J
R2483	T4091	T4087	punct	),1J
R2484	T4092	T4023	punct	.,are
R2485	T4094	T4095	prep	In,showed
R2486	T4096	T4094	pobj	contrast,In
R2487	T4097	T4095	punct	", ",showed
R2488	T4098	T4095	nsubj	mice,showed
R2489	T4099	T4100	prep	in,removed
R249	T420	T412	prep	in,results
R2490	T4100	T4098	relcl	removed,mice
R2491	T4101	T4099	pobj	which,in
R2492	T4102	T4103	det	both,copies
R2493	T4103	T4100	nsubjpass	copies,removed
R2494	T4104	T4103	prep	of,copies
R2495	T4105	T4104	pobj	Bmp2,of
R2496	T4106	T4103	cc	and,copies
R2497	T4107	T4108	nummod	one,copy
R2498	T4108	T4103	conj	copy,copies
R2499	T4109	T4108	prep	of,copy
R25	T182	T175	prep	including,members
R250	T421	T422	det	a,impairment
R2500	T4110	T4109	pobj	Bmp4,of
R2501	T4111	T4100	aux	had,removed
R2502	T4112	T4100	auxpass	been,removed
R2503	T4113	T4098	punct	(,mice
R2504	T4114	T4115	compound	Bmp2C,C
R2505	T4115	T4098	appos	C,mice
R2506	T4116	T4115	punct	/,C
R2507	T4117	T4115	punct	;,C
R2508	T4118	T4119	nmod	Bmp4,C
R2509	T4119	T4115	appos	C,C
R251	T422	T420	pobj	impairment,in
R2510	T4120	T4119	punct	+,C
R2511	T4121	T4119	punct	/,C
R2512	T4122	T4115	punct	;,C
R2513	T4123	T4124	nmod	Prx1,cre
R2514	T4124	T4115	appos	cre,C
R2515	T4125	T4124	punct	:,cre
R2516	T4126	T4124	punct	:,cre
R2517	T4127	T4098	punct	),mice
R2518	T4128	T4129	advmod	more,severe
R2519	T4129	T4130	amod	severe,defects
R252	T423	T422	amod	severe,impairment
R2520	T4130	T4095	dobj	defects,showed
R2521	T4131	T4130	amod	skeletal,defects
R2522	T4132	T4130	punct	", ",defects
R2523	T4133	T4130	prep	including,defects
R2524	T4134	T4135	advmod	significantly,thinner
R2525	T4135	T4136	amod	thinner,elements
R2526	T4136	T4133	pobj	elements,including
R2527	T4137	T4136	amod	skeletal,elements
R2528	T4138	T4095	punct	.,showed
R2529	T4140	T4141	advmod	However,are
R253	T424	T422	prep	of,impairment
R2530	T4142	T4141	punct	", ",are
R2531	T4143	T4144	det	the,patterns
R2532	T4144	T4141	nsubj	patterns,are
R2533	T4145	T4144	compound	digit,patterns
R2534	T4146	T4144	prep	of,patterns
R2535	T4147	T4148	det	those,animals
R2536	T4148	T4146	pobj	animals,of
R2537	T4149	T4150	advmod	completely,normal
R2538	T4150	T4141	acomp	normal,are
R2539	T4151	T4152	punct	(,1I
R254	T425	T424	pobj	osteogenesis,of
R2540	T4152	T4141	parataxis	1I,are
R2541	T4153	T4152	compound	Figure,1I
R2542	T4154	T4152	cc	and,1I
R2543	T4155	T4152	conj	1Q,1I
R2544	T4156	T4152	punct	),1I
R2545	T4157	T4141	punct	.,are
R2546	T4159	T4160	nsubj	Animals,had
R2547	T4161	T4162	prep	in,removed
R2548	T4162	T4159	relcl	removed,Animals
R2549	T4163	T4161	pobj	which,in
R255	T426	T407	punct	.,find
R2550	T4164	T4165	det	both,copies
R2551	T4165	T4162	nsubjpass	copies,removed
R2552	T4166	T4165	prep	of,copies
R2553	T4167	T4166	pobj	Bmp2,of
R2554	T4168	T4167	cc	and,Bmp2
R2555	T4169	T4167	conj	Bmp4,Bmp2
R2556	T4170	T4162	auxpass	were,removed
R2557	T4171	T4159	punct	(,Animals
R2558	T4172	T4173	compound	Bmp2C,C
R2559	T4173	T4159	appos	C,Animals
R256	T152	T153	amod	Genetic,Analysis
R2560	T4174	T4173	punct	/,C
R2561	T4175	T4173	punct	;,C
R2562	T4176	T4177	compound	Bmp4C,C
R2563	T4177	T4173	appos	C,C
R2564	T4178	T4177	punct	/,C
R2565	T4179	T4173	punct	;,C
R2566	T4180	T4181	compound	Prx1,cre
R2567	T4181	T4173	appos	cre,C
R2568	T4182	T4181	punct	:,cre
R2569	T4183	T4181	punct	:,cre
R2570	T4184	T4159	punct	),Animals
R2571	T4185	T4186	advmod	extremely,malformed
R2572	T4186	T4187	amod	malformed,limbs
R2573	T4187	T4160	dobj	limbs,had
R2574	T4188	T4189	punct	(,1K
R2575	T4189	T4160	parataxis	1K,had
R2576	T4190	T4189	compound	Figure,1K
R2577	T4191	T4189	cc	and,1K
R2578	T4192	T4189	conj	1S,1K
R2579	T4193	T4189	punct	),1K
R2580	T4194	T4160	punct	.,had
R2581	T4196	T4197	nsubj	They,displayed
R2582	T4197	T4198	ccomp	displayed,was
R2583	T4199	T4200	advmod	severely,short
R2584	T4200	T4201	amod	short,stylopods
R2585	T4201	T4197	dobj	stylopods,displayed
R2586	T4202	T4200	cc	and,short
R2587	T4203	T4200	conj	malformed,short
R2588	T4204	T4198	punct	;,was
R2589	T4205	T4198	nsubj	one,was
R2590	T4206	T4205	prep	of,one
R2591	T4207	T4208	det	the,elements
R2592	T4208	T4206	pobj	elements,of
R2593	T4209	T4208	compound	zeugopod,elements
R2594	T4210	T4211	advmod	almost,always
R2595	T4211	T4198	advmod	always,was
R2596	T4212	T4198	acomp	missing,was
R2597	T4213	T4198	punct	", ",was
R2598	T4214	T4198	cc	and,was
R2599	T4215	T4216	det	the,one
R26	T183	T182	pobj	BMP2,including
R2600	T4216	T4218	nsubj	one,was
R2601	T4217	T4216	amod	remaining,one
R2602	T4218	T4198	conj	was,was
R2603	T4219	T4220	advmod	so,deformed
R2604	T4220	T4218	acomp	deformed,was
R2605	T4221	T4222	mark	that,was
R2606	T4222	T4220	ccomp	was,deformed
R2607	T4223	T4222	nsubj	it,was
R2608	T4224	T4222	acomp	difficult,was
R2609	T4225	T4226	aux	to,identify
R2610	T4226	T4222	xcomp	identify,was
R2611	T4227	T4228	det	the,element
R2612	T4228	T4226	dobj	element,identify
R2613	T4229	T4226	advmod	correctly,identify
R2614	T4230	T4198	punct	.,was
R2615	T4232	T4233	advmod	Moreover,are
R2616	T4234	T4233	punct	", ",are
R2617	T4235	T4236	det	the,articulations
R2618	T4236	T4233	nsubj	articulations,are
R2619	T4237	T4236	compound	joint,articulations
R2620	T4238	T4233	acomp	defective,are
R2621	T4239	T4240	amod	such,fused
R2622	T4240	T4233	advcl	fused,are
R2623	T4241	T4240	mark	that,fused
R2624	T4242	T4243	nmod	zeugopod,elements
R2625	T4243	T4240	nsubjpass	elements,fused
R2626	T4244	T4242	cc	and,zeugopod
R2627	T4245	T4242	conj	stylopod,zeugopod
R2628	T4246	T4240	auxpass	are,fused
R2629	T4247	T4248	punct	(,see
R2630	T4248	T4233	parataxis	see,are
R2631	T4249	T4250	advmod	below,S1A
R2632	T4250	T4248	dobj	S1A,see
R2633	T4251	T4250	cc	and,S1A
R2634	T4252	T4250	compound	Figure,S1A
R2635	T4253	T4254	punct	–,S1C
R2636	T4254	T4250	prep	S1C,S1A
R2637	T4255	T4248	punct	),see
R2638	T4256	T4233	punct	.,are
R2639	T4258	T4259	advmod	Interestingly,are
R2640	T4260	T4259	punct	", ",are
R2641	T4261	T4262	det	the,autopods
R2642	T4262	T4259	nsubj	autopods,are
R2643	T4263	T4264	advmod	less,affected
R2644	T4264	T4259	acomp	affected,are
R2645	T4265	T4264	prep	than,affected
R2646	T4266	T4267	det	the,elements
R2647	T4267	T4265	pobj	elements,than
R2648	T4268	T4267	amod	proximal,elements
R2649	T4269	T4259	punct	.,are
R2650	T4271	T4272	advmod	Nonetheless,are
R2651	T4273	T4272	punct	", ",are
R2652	T4274	T4275	det	the,elements
R2653	T4275	T4272	nsubj	elements,are
R2654	T4276	T4275	compound	autopod,elements
R2655	T4277	T4278	advmod	significantly,reduced
R2656	T4278	T4272	acomp	reduced,are
R2657	T4279	T4278	prep	in,reduced
R2658	T4280	T4279	pobj	size,in
R2659	T4281	T4272	punct	", ",are
R2660	T4282	T4272	cc	and,are
R2661	T4283	T4284	advmod	strikingly,are
R2662	T4284	T4272	conj	are,are
R2663	T4285	T4286	det	the,digits
R2664	T4286	T4284	nsubj	digits,are
R2665	T4287	T4286	nummod	two,digits
R2666	T4288	T4286	amod	posterior-most,digits
R2667	T4289	T4284	acomp	missing,are
R2668	T4290	T4284	prep	in,are
R2669	T4291	T4292	det	the,forelimbs
R2670	T4292	T4290	pobj	forelimbs,in
R2671	T4293	T4292	prep	of,forelimbs
R2672	T4294	T4295	det	these,animals
R2673	T4295	T4293	pobj	animals,of
R2674	T4296	T4284	punct	.,are
R2675	T4298	T4299	amod	Simultaneous,removal
R2676	T4299	T4300	nsubj	removal,had
R2677	T4301	T4299	prep	of,removal
R2678	T4302	T4301	pobj	BMP2,of
R2679	T4303	T4302	cc	and,BMP2
R2680	T4304	T4302	conj	BMP7,BMP2
R2681	T4305	T4306	advmod	far,less
R2682	T4306	T4300	dobj	less,had
R2683	T4307	T4306	prep	of,less
R2684	T4308	T4309	det	an,effect
R2685	T4309	T4307	pobj	effect,of
R2686	T4310	T4311	mark	than,did
R2687	T4311	T4306	advcl	did,less
R2688	T4312	T4311	nsubj	removal,did
R2689	T4313	T4312	prep	of,removal
R2690	T4314	T4315	nmod	BMP2,activity
R2691	T4315	T4313	pobj	activity,of
R2692	T4316	T4314	cc	and,BMP2
R2693	T4317	T4314	conj	BMP4,BMP2
R2694	T4318	T4300	punct	.,had
R2695	T4320	T4321	nmod	Compound,removal
R2696	T4321	T4323	nsubj	removal,were
R2697	T4322	T4321	amod	heterozygous,removal
R2698	T4324	T4321	prep	of,removal
R2699	T4325	T4324	pobj	BMP2,of
R27	T184	T183	punct	", ",BMP2
R2700	T4326	T4325	cc	and,BMP2
R2701	T4327	T4325	conj	BMP7,BMP2
R2702	T4328	T4329	punct	(,C
R2703	T4329	T4327	parataxis	C,BMP7
R2704	T4330	T4329	nmod	Bmp2,C
R2705	T4331	T4329	punct	+,C
R2706	T4332	T4329	punct	/,C
R2707	T4333	T4329	punct	", ",C
R2708	T4334	T4329	appos	Bmp7,C
R2709	T4335	T4334	punct	+,Bmp7
R271	T1147	T1148	nmod	Bone,proteins
R2710	T4336	T4334	punct	/,Bmp7
R2711	T4337	T4334	punct	−,Bmp7
R2712	T4338	T4329	punct	;,C
R2713	T4339	T4340	compound	Prx1,cre
R2714	T4340	T4329	appos	cre,C
R2715	T4341	T4340	punct	:,cre
R2716	T4342	T4340	punct	:,cre
R2717	T4343	T4329	punct	),C
R2718	T4344	T4321	cc	and,removal
R2719	T4345	T4346	amod	heterozygous,removal
R272	T1148	T1150	nsubj	proteins,are
R2720	T4346	T4321	conj	removal,removal
R2721	T4347	T4346	prep	of,removal
R2722	T4348	T4347	pobj	BMP2,of
R2723	T4349	T4346	prep	with,removal
R2724	T4350	T4351	amod	complete,removal
R2725	T4351	T4349	pobj	removal,with
R2726	T4352	T4351	prep	of,removal
R2727	T4353	T4352	pobj	BMP7,of
R2728	T4354	T4353	punct	(,BMP7
R2729	T4355	T4356	nmod	Bmp2,C
R273	T1149	T1148	amod	morphogenetic,proteins
R2730	T4356	T4353	nmod	C,BMP7
R2731	T4357	T4356	punct	+,C
R2732	T4358	T4356	punct	/,C
R2733	T4359	T4356	punct	", ",C
R2734	T4360	T4356	appos	Bmp7,C
R2735	T4361	T4360	punct	−,Bmp7
R2736	T4362	T4360	punct	/,Bmp7
R2737	T4363	T4360	punct	−,Bmp7
R2738	T4364	T4356	punct	;,C
R2739	T4365	T4366	compound	Prx1,cre
R274	T1151	T1148	punct	(,proteins
R2740	T4366	T4356	appos	cre,C
R2741	T4367	T4366	punct	:,cre
R2742	T4368	T4366	punct	:,cre
R2743	T4369	T4353	punct	),BMP7
R2744	T4370	T4323	advmod	both,were
R2745	T4371	T4372	advmod	completely,type
R2746	T4372	T4323	attr	type,were
R2747	T4373	T4372	amod	wild,type
R2748	T4374	T4372	punct	-,type
R2749	T4375	T4323	prep	in,were
R275	T1152	T1148	appos	BMPs,proteins
R2750	T4376	T4377	amod	skeletal,pattern
R2751	T4377	T4375	pobj	pattern,in
R2752	T4378	T4377	cc	and,pattern
R2753	T4379	T4377	conj	differentiation,pattern
R2754	T4380	T4381	punct	(,data
R2755	T4381	T4323	meta	data,were
R2756	T4382	T4381	amod	unpublished,data
R2757	T4383	T4381	punct	),data
R2758	T4384	T4323	punct	.,were
R2759	T4386	T4387	nsubj	Mice,displayed
R276	T1153	T1150	punct	),are
R2760	T4388	T4386	amod	homozygous,Mice
R2761	T4389	T4388	prep	for,homozygous
R2762	T4390	T4389	pobj	removal,for
R2763	T4391	T4390	prep	of,removal
R2764	T4392	T4391	pobj	Bmp2,of
R2765	T4393	T4388	cc	and,homozygous
R2766	T4394	T4388	conj	heterozygous,homozygous
R2767	T4395	T4394	prep	for,heterozygous
R2768	T4396	T4395	pobj	Bmp7,for
R2769	T4397	T4398	punct	(,C
R277	T1154	T1155	amod	secreted,molecules
R2770	T4398	T4394	parataxis	C,heterozygous
R2771	T4399	T4398	compound	Bmp2C,C
R2772	T4400	T4398	punct	/,C
R2773	T4401	T4398	punct	", ",C
R2774	T4402	T4398	appos	Bmp7,C
R2775	T4403	T4402	punct	+,Bmp7
R2776	T4404	T4402	punct	/,Bmp7
R2777	T4405	T4402	punct	−,Bmp7
R2778	T4406	T4398	punct	;,C
R2779	T4407	T4408	compound	Prx1,cre
R278	T1155	T1150	attr	molecules,are
R2780	T4408	T4398	appos	cre,C
R2781	T4409	T4408	punct	:,cre
R2782	T4410	T4408	punct	:,cre
R2783	T4411	T4398	punct	),C
R2784	T4412	T4413	det	the,defect
R2785	T4413	T4387	dobj	defect,displayed
R2786	T4414	T4413	amod	same,defect
R2787	T4415	T4413	amod	subtle,defect
R2788	T4416	T4413	amod	scapular,defect
R2789	T4417	T4413	acl	seen,defect
R279	T1156	T1155	compound	signaling,molecules
R2790	T4418	T4417	prep	in,seen
R2791	T4419	T4420	nmod	Bmp2C,C
R2792	T4420	T4422	nmod	C,mutants
R2793	T4421	T4420	punct	/,C
R2794	T4422	T4418	pobj	mutants,in
R2795	T4423	T4420	punct	;,C
R2796	T4424	T4425	compound	Prx1,cre
R2797	T4425	T4420	appos	cre,C
R2798	T4426	T4425	punct	:,cre
R2799	T4427	T4425	punct	:,cre
R28	T185	T183	conj	BMP4,BMP2
R280	T1157	T1155	acl	belonging,molecules
R2800	T4428	T4422	advmod	alone,mutants
R2801	T4429	T4430	punct	(,data
R2802	T4430	T4387	meta	data,displayed
R2803	T4431	T4430	amod	unpublished,data
R2804	T4432	T4430	punct	),data
R2805	T4433	T4387	punct	.,displayed
R2806	T4435	T4436	prep	In,was
R2807	T4437	T4435	pobj	limbs,In
R2808	T4438	T4437	acl	developing,limbs
R2809	T4439	T4438	prep	in,developing
R281	T1158	T1157	prep	to,belonging
R2810	T4440	T4441	det	the,absence
R2811	T4441	T4439	pobj	absence,in
R2812	T4442	T4441	amod	complete,absence
R2813	T4443	T4441	prep	of,absence
R2814	T4444	T4445	preconj	both,BMP7
R2815	T4445	T4446	nmod	BMP7,activity
R2816	T4446	T4443	pobj	activity,of
R2817	T4447	T4445	cc	and,BMP7
R2818	T4448	T4445	conj	BMP2,BMP7
R2819	T4449	T4450	punct	(,C
R282	T1159	T1160	det	the,superfamily
R2820	T4450	T4437	parataxis	C,limbs
R2821	T4451	T4450	compound	Bmp2C,C
R2822	T4452	T4450	punct	/,C
R2823	T4453	T4450	punct	", ",C
R2824	T4454	T4450	appos	Bmp7,C
R2825	T4455	T4454	punct	−,Bmp7
R2826	T4456	T4454	punct	/,Bmp7
R2827	T4457	T4454	punct	−,Bmp7
R2828	T4458	T4450	punct	;,C
R2829	T4459	T4460	compound	Prx1,cre
R283	T1160	T1158	pobj	superfamily,to
R2830	T4460	T4450	appos	cre,C
R2831	T4461	T4460	punct	:,cre
R2832	T4462	T4460	punct	:,cre
R2833	T4463	T4450	punct	),C
R2834	T4464	T4436	punct	", ",was
R2835	T4465	T4466	det	the,phalanx
R2836	T4466	T4436	nsubj	phalanx,was
R2837	T4467	T4466	amod	last,phalanx
R2838	T4468	T4436	acomp	missing,was
R2839	T4469	T4468	prep	from,missing
R284	T1161	T1160	amod	transforming,superfamily
R2840	T4470	T4469	pobj	digit,from
R2841	T4471	T4470	nummod	III,digit
R2842	T4472	T4470	prep	in,digit
R2843	T4473	T4474	det	the,forelimb
R2844	T4474	T4472	pobj	forelimb,in
R2845	T4475	T4436	punct	", ",was
R2846	T4476	T4436	cc	and,was
R2847	T4477	T4478	det	these,limbs
R2848	T4478	T4479	nsubj	limbs,displayed
R2849	T4479	T4436	conj	displayed,was
R285	T1162	T1163	compound	growth,β
R2850	T4480	T4481	det	the,phenotype
R2851	T4481	T4479	dobj	phenotype,displayed
R2852	T4482	T4481	amod	same,phenotype
R2853	T4483	T4479	prep	in,displayed
R2854	T4484	T4485	det	the,hindlimb
R2855	T4485	T4483	pobj	hindlimb,in
R2856	T4486	T4479	prep	with,displayed
R2857	T4487	T4488	amod	variable,penetrance
R2858	T4488	T4486	pobj	penetrance,with
R2859	T4489	T4490	punct	(,arrow
R286	T1163	T1160	compound	β,superfamily
R2860	T4490	T4479	parataxis	arrow,displayed
R2861	T4491	T4492	compound	Figure,1L
R2862	T4492	T4490	dep	1L,arrow
R2863	T4493	T4492	cc	and,1L
R2864	T4494	T4492	conj	1T,1L
R2865	T4495	T4490	punct	", ",arrow
R2866	T4496	T4490	amod	black,arrow
R2867	T4497	T4490	punct	),arrow
R2868	T4498	T4479	punct	.,displayed
R2869	T4500	T4501	advmod	Additionally,are
R287	T1164	T1163	compound	factor,β
R2870	T4502	T4501	punct	", ",are
R2871	T4503	T4504	det	the,fibulae
R2872	T4504	T4501	nsubj	fibulae,are
R2873	T4505	T4504	prep	of,fibulae
R2874	T4506	T4507	det	these,hindlimbs
R2875	T4507	T4505	pobj	hindlimbs,of
R2876	T4508	T4501	acomp	malformed,are
R2877	T4509	T4501	cc	and,are
R2878	T4510	T4511	aux	do,articulate
R2879	T4511	T4501	conj	articulate,are
R288	T1165	T1160	punct	", ",superfamily
R2880	T4512	T4511	neg	not,articulate
R2881	T4513	T4511	prep	with,articulate
R2882	T4514	T4515	det	the,femur
R2883	T4515	T4513	pobj	femur,with
R2884	T4516	T4511	prep	at,articulate
R2885	T4517	T4518	det	the,knee
R2886	T4518	T4516	pobj	knee,at
R2887	T4519	T4520	punct	(,arrow
R2888	T4520	T4511	parataxis	arrow,articulate
R2889	T4521	T4522	compound	Figure,1T
R289	T1166	T1167	advmod	originally,identified
R2890	T4522	T4520	dep	1T,arrow
R2891	T4523	T4520	punct	", ",arrow
R2892	T4524	T4520	amod	thick,arrow
R2893	T4525	T4520	amod	red,arrow
R2894	T4526	T4520	punct	),arrow
R2895	T4527	T4501	punct	.,are
R2896	T4529	T4530	nsubj	They,display
R2897	T4531	T4530	advmod	also,display
R2898	T4532	T4533	det	the,defects
R2899	T4533	T4530	dobj	defects,display
R29	T186	T185	punct	", ",BMP4
R290	T1167	T1160	acl	identified,superfamily
R2900	T4534	T4533	amod	same,defects
R2901	T4535	T4533	amod	scapular,defects
R2902	T4536	T4533	acl	seen,defects
R2903	T4537	T4536	prep	in,seen
R2904	T4538	T4539	nmod	Bmp2C,C
R2905	T4539	T4541	nmod	C,mice
R2906	T4540	T4539	punct	/,C
R2907	T4541	T4537	pobj	mice,in
R2908	T4542	T4539	punct	;,C
R2909	T4543	T4544	compound	Prx1,cre
R291	T1168	T1167	prep	on,identified
R2910	T4544	T4539	appos	cre,C
R2911	T4545	T4544	punct	:,cre
R2912	T4546	T4544	punct	:,cre
R2913	T4547	T4530	punct	", ",display
R2914	T4548	T4530	cc	and,display
R2915	T4549	T4550	det	the,size
R2916	T4550	T4552	nsubj	size,is
R2917	T4551	T4550	amod	overall,size
R2918	T4552	T4530	conj	is,display
R2919	T4553	T4550	prep	of,size
R292	T1169	T1170	det	the,basis
R2920	T4554	T4555	det	the,skeleton
R2921	T4555	T4553	pobj	skeleton,of
R2922	T4556	T4555	amod	appendicular,skeleton
R2923	T4557	T4558	advmod	slightly,diminished
R2924	T4558	T4552	acomp	diminished,is
R2925	T4559	T4552	punct	.,is
R2926	T4561	T4562	advmod	However,appears
R2927	T4563	T4562	punct	", ",appears
R2928	T4564	T4565	amod	skeletal,differentiation
R2929	T4565	T4562	nsubj	differentiation,appears
R293	T1170	T1168	pobj	basis,on
R2930	T4566	T4562	oprd	normal,appears
R2931	T4567	T4562	prep	in,appears
R2932	T4568	T4569	det	the,limbs
R2933	T4569	T4567	pobj	limbs,in
R2934	T4570	T4569	prep	of,limbs
R2935	T4571	T4572	det	these,animals
R2936	T4572	T4570	pobj	animals,of
R2937	T4573	T4562	punct	.,appears
R294	T1171	T1170	prep	of,basis
R2946	T4773	T4774	amod	Soft,Tissue
R2947	T4774	T4775	compound	Tissue,Syndactyly
R2948	T4775	T4776	nsubj	Syndactyly,Observed
R2949	T4777	T4776	prep	in,Observed
R295	T1172	T1173	poss	their,ability
R2950	T4778	T4777	pobj	Limbs,in
R2951	T4779	T4778	prep	of,Limbs
R2952	T4780	T4779	pobj	Mice,of
R2953	T4781	T4780	amod	Devoid,Mice
R2954	T4782	T4781	prep	of,Devoid
R2955	T4783	T4784	nmod	BMP2,Activity
R2956	T4784	T4782	pobj	Activity,of
R2957	T4785	T4783	cc	and,BMP2
R2958	T4786	T4783	conj	BMP4,BMP2
R2959	T4788	T4789	mark	As,noted
R296	T1173	T1171	pobj	ability,of
R2960	T4789	T4790	advcl	noted,display
R2961	T4791	T4789	advmod	above,noted
R2962	T4792	T4790	punct	", ",display
R2963	T4793	T4794	npadvmod	Bmp2,deficient
R2964	T4794	T4796	amod	deficient,animals
R2965	T4795	T4794	punct	-,deficient
R2966	T4796	T4790	nsubj	animals,display
R2967	T4797	T4798	det	a,phenotype
R2968	T4798	T4790	dobj	phenotype,display
R2969	T4799	T4800	advmod	variably,penetrant
R297	T1174	T1175	aux	to,induce
R2970	T4800	T4798	amod	penetrant,phenotype
R2971	T4801	T4802	quantmod	3,4
R2972	T4802	T4804	nummod	4,tissue
R2973	T4803	T4802	punct	/,4
R2974	T4804	T4806	compound	tissue,syndactyly
R2975	T4805	T4804	amod	soft,tissue
R2976	T4806	T4798	compound	syndactyly,phenotype
R2977	T4807	T4808	punct	(,2B
R2978	T4808	T4790	parataxis	2B,display
R2979	T4809	T4808	compound	Figure,2B
R298	T1175	T1173	acl	induce,ability
R2980	T4810	T4808	punct	),2B
R2981	T4811	T4790	punct	.,display
R2982	T4813	T4814	prep	In,show
R2983	T4815	T4813	pobj	contrast,In
R2984	T4816	T4814	punct	", ",show
R2985	T4817	T4818	npadvmod	Bmp4,deficient
R2986	T4818	T4820	amod	deficient,mice
R2987	T4819	T4818	punct	-,deficient
R2988	T4820	T4814	nsubj	mice,show
R2989	T4821	T4822	det	no,evidence
R299	T1176	T1177	amod	ectopic,formation
R2990	T4822	T4814	dobj	evidence,show
R2991	T4823	T4822	prep	of,evidence
R2992	T4824	T4823	pobj	syndactyly,of
R2993	T4825	T4826	punct	(,data
R2994	T4826	T4814	meta	data,show
R2995	T4827	T4826	amod	unpublished,data
R2996	T4828	T4826	punct	),data
R2997	T4829	T4814	punct	.,show
R2998	T4831	T4832	advmod	However,is
R2999	T4833	T4832	punct	", ",is
R3	T157	T155	pobj	Roles,of
R30	T187	T185	cc	and,BMP4
R300	T1177	T1175	dobj	formation,induce
R3000	T4834	T4832	nsubj	one,is
R3001	T4835	T4834	prep	of,one
R3002	T4836	T4837	det	the,defects
R3003	T4837	T4835	pobj	defects,of
R3004	T4838	T4837	amod	striking,defects
R3005	T4839	T4834	acl	observed,one
R3006	T4840	T4839	prep	in,observed
R3007	T4841	T4842	det	the,mice
R3008	T4842	T4840	pobj	mice,in
R3009	T4843	T4844	nmod	Bmp2C,C
R301	T1178	T1177	compound	bone,formation
R3010	T4844	T4842	nmod	C,mice
R3011	T4845	T4844	punct	/,C
R3012	T4846	T4844	punct	;,C
R3013	T4847	T4848	compound	Bmp4C,C
R3014	T4848	T4844	appos	C,C
R3015	T4849	T4848	punct	/,C
R3016	T4850	T4844	punct	;,C
R3017	T4851	T4852	compound	Prx1,cre
R3018	T4852	T4844	appos	cre,C
R3019	T4853	T4852	punct	:,cre
R302	T1179	T1180	advmod	when,implanted
R3020	T4854	T4852	punct	:,cre
R3021	T4855	T4856	mark	that,show
R3022	T4856	T4832	ccomp	show,is
R3023	T4857	T4856	prep	in,show
R3024	T4858	T4859	det	the,animals
R3025	T4859	T4857	pobj	animals,in
R3026	T4860	T4859	amod	newborn,animals
R3027	T4861	T4856	punct	", ",show
R3028	T4862	T4863	det	the,digits
R3029	T4863	T4856	nsubj	digits,show
R303	T1180	T1175	advcl	implanted,induce
R3030	T4864	T4863	prep	of,digits
R3031	T4865	T4866	preconj	both,forelimb
R3032	T4866	T4864	pobj	forelimb,of
R3033	T4867	T4868	punct	(,data
R3034	T4868	T4866	meta	data,forelimb
R3035	T4869	T4868	amod	unpublished,data
R3036	T4870	T4868	punct	),data
R3037	T4871	T4866	cc	and,forelimb
R3038	T4872	T4866	conj	hindlimb,forelimb
R3039	T4873	T4874	amod	complete,syndactyly
R304	T1181	T1180	prep	within,implanted
R3040	T4874	T4856	dobj	syndactyly,show
R3041	T4875	T4876	punct	(,2D
R3042	T4876	T4832	parataxis	2D,is
R3043	T4877	T4876	compound	Figure,2D
R3044	T4878	T4876	punct	;,2D
R3045	T4879	T4876	advcl	compare,2D
R3046	T4880	T4879	prep	to,compare
R3047	T4881	T4882	amod	wild,type
R3048	T4882	T4880	pobj	type,to
R3049	T4883	T4882	punct	-,type
R305	T1182	T1183	amod	soft,tissue
R3050	T4884	T4882	punct	", ",type
R3051	T4885	T4886	compound	Figure,2C
R3052	T4886	T4882	npadvmod	2C,type
R3053	T4887	T4876	punct	),2D
R3054	T4888	T4832	punct	.,is
R3055	T4890	T4891	aux	To,determine
R3056	T4891	T4892	advcl	determine,examined
R3057	T4893	T4894	det	the,origin
R3058	T4894	T4891	dobj	origin,determine
R3059	T4895	T4894	prep	of,origin
R306	T1183	T1181	pobj	tissue,within
R3060	T4896	T4897	det	this,defect
R3061	T4897	T4895	pobj	defect,of
R3062	T4898	T4892	punct	", ",examined
R3063	T4899	T4892	nsubj	we,examined
R3064	T4900	T4901	amod	embryonic,limbs
R3065	T4901	T4892	dobj	limbs,examined
R3066	T4902	T4892	prep	at,examined
R3067	T4903	T4902	pobj	E15.5,at
R3068	T4904	T4903	punct	", ",E15.5
R3069	T4905	T4906	det	the,stage
R307	T1184	T1185	advmod	in,vivo
R3070	T4906	T4903	appos	stage,E15.5
R3071	T4907	T4908	advmod	when,taking
R3072	T4908	T4906	relcl	taking,stage
R3073	T4909	T4910	amod	interdigital,separation
R3074	T4910	T4908	nsubj	separation,taking
R3075	T4911	T4908	aux	is,taking
R3076	T4912	T4908	advmod	normally,taking
R3077	T4913	T4908	dobj	place,taking
R3078	T4914	T4915	punct	(,2E
R3079	T4915	T4892	parataxis	2E,examined
R308	T1185	T1180	advmod	vivo,implanted
R3080	T4916	T4915	compound	Figure,2E
R3081	T4917	T4915	punct	),2E
R3082	T4918	T4892	punct	.,examined
R3083	T4920	T4921	prep	In,separated
R3084	T4922	T4923	nmod	Bmp2C,C
R3085	T4923	T4925	nmod	C,limbs
R3086	T4924	T4923	punct	/,C
R3087	T4925	T4920	pobj	limbs,In
R3088	T4926	T4923	punct	;,C
R3089	T4927	T4928	compound	Bmp4C,C
R309	T1186	T1187	punct	[,1
R3090	T4928	T4923	appos	C,C
R3091	T4929	T4928	punct	/,C
R3092	T4930	T4923	punct	;,C
R3093	T4931	T4932	compound	Prx1,cre
R3094	T4932	T4923	appos	cre,C
R3095	T4933	T4932	punct	:,cre
R3096	T4934	T4932	punct	:,cre
R3097	T4935	T4921	punct	", ",separated
R3098	T4936	T4937	advmod	only,tips
R3099	T4937	T4921	nsubjpass	tips,separated
R31	T188	T185	conj	BMP7,BMP4
R310	T1187	T1150	parataxis	1,are
R3100	T4938	T4937	det	the,tips
R3101	T4939	T4940	advmod	very,distal
R3102	T4940	T4937	amod	distal,tips
R3103	T4941	T4937	prep	of,tips
R3104	T4942	T4943	det	each,digit
R3105	T4943	T4941	pobj	digit,of
R3106	T4944	T4921	auxpass	were,separated
R3107	T4945	T4921	punct	", ",separated
R3108	T4946	T4921	prep	with,separated
R3109	T4947	T4948	det	the,autopod
R311	T1188	T1189	punct	–,3
R3110	T4948	T4949	nsubj	autopod,adopting
R3111	T4949	T4946	pcomp	adopting,with
R3112	T4950	T4951	det	the,shape
R3113	T4951	T4949	dobj	shape,adopting
R3114	T4952	T4951	prep	of,shape
R3115	T4953	T4954	det	a,pallet
R3116	T4954	T4952	pobj	pallet,of
R3117	T4955	T4954	amod	notched,pallet
R3118	T4956	T4957	punct	(,2F
R3119	T4957	T4921	parataxis	2F,separated
R312	T1189	T1187	prep	3,1
R3120	T4958	T4957	compound	Figure,2F
R3121	T4959	T4957	punct	),2F
R3122	T4960	T4921	punct	.,separated
R3123	T4962	T4963	prep	In,become
R3124	T4964	T4965	amod	wild,type
R3125	T4965	T4967	compound	type,development
R3126	T4966	T4965	punct	-,type
R3127	T4967	T4962	pobj	development,In
R3128	T4968	T4963	punct	", ",become
R3129	T4969	T4970	det	the,digits
R313	T1190	T1187	punct	],1
R3130	T4970	T4963	nsubj	digits,become
R3131	T4971	T4970	amod	individual,digits
R3132	T4972	T4963	acomp	free,become
R3133	T4973	T4972	prep	from,free
R3134	T4974	T4973	pobj	one,from
R3135	T4975	T4974	det	another,one
R3136	T4976	T4963	prep	through,become
R3137	T4977	T4978	det	a,process
R3138	T4978	T4976	pobj	process,through
R3139	T4979	T4978	prep	of,process
R314	T1191	T1150	punct	.,are
R3140	T4980	T4981	amod	interdigital,apoptosis
R3141	T4981	T4979	pobj	apoptosis,of
R3142	T4982	T4978	punct	", ",process
R3143	T4983	T4978	amod	observable,process
R3144	T4984	T4983	prep	by,observable
R3145	T4985	T4984	pcomp	staining,by
R3146	T4986	T4985	prep	with,staining
R3147	T4987	T4988	amod	acridine,orange
R3148	T4988	T4986	pobj	orange,with
R3149	T4989	T4990	punct	(,arrow
R315	T1193	T1194	compound	BMP,members
R3150	T4990	T4963	parataxis	arrow,become
R3151	T4991	T4992	compound	Figure,2E
R3152	T4992	T4990	dep	2E,arrow
R3153	T4993	T4992	cc	and,2E
R3154	T4994	T4992	conj	2G,2E
R3155	T4995	T4990	punct	", ",arrow
R3156	T4996	T4990	punct	),arrow
R3157	T4997	T4963	punct	.,become
R3158	T4999	T5000	amod	Interdigital,apoptosis
R3159	T5000	T5001	nsubjpass	apoptosis,reduced
R316	T1194	T1196	nsubjpass	members,known
R3160	T5002	T5001	auxpass	is,reduced
R3161	T5003	T5001	advmod	dramatically,reduced
R3162	T5004	T5001	prep	in,reduced
R3163	T5005	T5006	det	the,limbs
R3164	T5006	T5004	pobj	limbs,in
R3165	T5007	T5008	nmod	Bmp2C,C
R3166	T5008	T5006	nmod	C,limbs
R3167	T5009	T5008	punct	/,C
R3168	T5010	T5008	punct	;,C
R3169	T5011	T5012	compound	Bmp4C,C
R317	T1195	T1194	compound	family,members
R3170	T5012	T5008	appos	C,C
R3171	T5013	T5012	punct	/,C
R3172	T5014	T5008	punct	;,C
R3173	T5015	T5016	compound	Prx1,cre
R3174	T5016	T5008	appos	cre,C
R3175	T5017	T5016	punct	:,cre
R3176	T5018	T5016	punct	:,cre
R3177	T5019	T5020	punct	(,arrow
R3178	T5020	T5001	parataxis	arrow,reduced
R3179	T5021	T5022	compound	Figure,2F
R318	T1197	T1196	auxpass	are,known
R3180	T5022	T5020	dep	2F,arrow
R3181	T5023	T5022	cc	and,2F
R3182	T5024	T5022	conj	2H,2F
R3183	T5025	T5020	punct	", ",arrow
R3184	T5026	T5020	punct	),arrow
R3185	T5027	T5001	punct	.,reduced
R3186	T5029	T5030	punct	(,Note
R3187	T5031	T5032	mark	that,is
R3188	T5032	T5030	ccomp	is,Note
R3189	T5033	T5034	det	the,staining
R319	T1198	T1196	advmod	now,known
R3190	T5034	T5032	nsubj	staining,is
R3191	T5035	T5034	amod	strong,staining
R3192	T5036	T5037	compound	acridine,orange
R3193	T5037	T5034	compound	orange,staining
R3194	T5038	T5034	prep	in,staining
R3195	T5039	T5040	compound	Figure,2H
R3196	T5040	T5038	pobj	2H,in
R3197	T5041	T5032	neg	not,is
R3198	T5042	T5032	prep	in,is
R3199	T5043	T5044	det	the,mesenchyme
R32	T189	T180	punct	", ",expressed
R320	T1199	T1200	aux	to,play
R3200	T5044	T5042	pobj	mesenchyme,in
R3201	T5045	T5044	amod	interdigital,mesenchyme
R3202	T5046	T5032	cc	but,is
R3203	T5047	T5046	advmod	rather,but
R3204	T5048	T5032	conj	is,is
R3205	T5049	T5048	prep	in,is
R3206	T5050	T5051	det	the,remnant
R3207	T5051	T5049	pobj	remnant,in
R3208	T5052	T5051	prep	of,remnant
R3209	T5053	T5054	det	the,cells
R321	T1200	T1196	xcomp	play,known
R3210	T5054	T5052	pobj	cells,of
R3211	T5055	T5054	prep	of,cells
R3212	T5056	T5057	det	the,AER
R3213	T5057	T5055	pobj	AER,of
R3214	T5058	T5059	punct	[,asterisk
R3215	T5059	T5030	parataxis	asterisk,Note
R3216	T5060	T5059	amod	red,asterisk
R3217	T5061	T5059	punct	],asterisk
R3218	T5062	T5030	punct	.,Note
R3219	T5063	T5030	punct	),Note
R322	T1201	T1202	det	an,set
R3220	T5065	T5066	det	Another,region
R3221	T5066	T5067	nsubj	region,is
R3222	T5068	T5066	prep	of,region
R3223	T5069	T5070	advmod	very,prominent
R3224	T5070	T5071	amod	prominent,apoptosis
R3225	T5071	T5068	pobj	apoptosis,of
R3226	T5072	T5066	prep	in,region
R3227	T5073	T5074	det	the,bud
R3228	T5074	T5072	pobj	bud,in
R3229	T5075	T5076	amod	wild,type
R323	T1202	T1200	dobj	set,play
R3230	T5076	T5074	compound	type,bud
R3231	T5077	T5076	punct	-,type
R3232	T5078	T5074	compound	limb,bud
R3233	T5079	T5067	prep	within,is
R3234	T5080	T5081	det	the,AER
R3235	T5081	T5079	pobj	AER,within
R3236	T5082	T5067	punct	.,is
R3237	T5084	T5085	det	The,AER
R3238	T5085	T5086	nsubj	AER,is
R3239	T5087	T5088	det	a,ridge
R324	T1203	T1204	advmod	extremely,diverse
R3240	T5088	T5086	attr	ridge,is
R3241	T5089	T5088	amod	specialized,ridge
R3242	T5090	T5088	prep	of,ridge
R3243	T5091	T5090	pobj	ectoderm,of
R3244	T5092	T5088	acl	running,ridge
R3245	T5093	T5092	prep	along,running
R3246	T5094	T5095	det	the,axis
R3247	T5095	T5093	pobj	axis,along
R3248	T5096	T5097	amod	anterior,posterior
R3249	T5097	T5095	amod	posterior,axis
R325	T1204	T1202	amod	diverse,set
R3250	T5098	T5097	punct	-,posterior
R3251	T5099	T5095	prep	at,axis
R3252	T5100	T5101	det	the,margin
R3253	T5101	T5099	pobj	margin,at
R3254	T5102	T5101	amod	distal,margin
R3255	T5103	T5101	prep	of,margin
R3256	T5104	T5105	det	the,bud
R3257	T5105	T5103	pobj	bud,of
R3258	T5106	T5105	amod	early,bud
R3259	T5107	T5105	compound	limb,bud
R326	T1205	T1202	prep	of,set
R3260	T5108	T5086	punct	.,is
R3261	T5110	T5111	nsubj	One,is
R3262	T5112	T5110	prep	of,One
R3263	T5113	T5114	det	the,phenotypes
R3264	T5114	T5112	pobj	phenotypes,of
R3265	T5115	T5110	acl	characterizing,One
R3266	T5116	T5117	det	the,mutant
R3267	T5117	T5115	dobj	mutant,characterizing
R3268	T5118	T5119	nmod	Bmp2C,C
R3269	T5119	T5117	nmod	C,mutant
R327	T1206	T1205	pobj	roles,of
R3270	T5120	T5119	punct	/,C
R3271	T5121	T5119	punct	;,C
R3272	T5122	T5123	compound	Bmp4C,C
R3273	T5123	T5119	appos	C,C
R3274	T5124	T5123	punct	/,C
R3275	T5125	T5119	punct	;,C
R3276	T5126	T5127	compound	Prx1,cre
R3277	T5127	T5119	appos	cre,C
R3278	T5128	T5127	punct	:,cre
R3279	T5129	T5127	punct	:,cre
R328	T1207	T1200	prep	in,play
R3280	T5130	T5111	attr	expansion,is
R3281	T5131	T5130	prep	of,expansion
R3282	T5132	T5133	det	the,AER
R3283	T5133	T5131	pobj	AER,of
R3284	T5134	T5135	punct	(,see
R3285	T5135	T5111	parataxis	see,is
R3286	T5136	T5135	advmod	below,see
R3287	T5137	T5135	punct	),see
R3288	T5138	T5111	punct	.,is
R3289	T5140	T5141	advmod	Moreover,maintained
R329	T1208	T1209	det	a,variety
R3290	T5142	T5141	punct	", ",maintained
R3291	T5143	T5141	prep	in,maintained
R3292	T5144	T5145	det	the,animals
R3293	T5145	T5143	pobj	animals,in
R3294	T5146	T5147	nmod	Bmp2C,C
R3295	T5147	T5145	nmod	C,animals
R3296	T5148	T5147	punct	/,C
R3297	T5149	T5147	punct	;,C
R3298	T5150	T5151	compound	Bmp4C,C
R3299	T5151	T5147	appos	C,C
R33	T190	T180	auxpass	are,expressed
R330	T1209	T1207	pobj	variety,in
R3300	T5152	T5151	punct	/,C
R3301	T5153	T5147	punct	;,C
R3302	T5154	T5155	compound	Prx1,cre
R3303	T5155	T5147	appos	cre,C
R3304	T5156	T5155	punct	:,cre
R3305	T5157	T5155	punct	:,cre
R3306	T5158	T5141	punct	", ",maintained
R3307	T5159	T5160	det	the,AER
R3308	T5160	T5141	nsubjpass	AER,maintained
R3309	T5161	T5141	auxpass	is,maintained
R331	T1210	T1209	amod	wide,variety
R3310	T5162	T5163	advmod	as,late
R3311	T5163	T5141	advmod	late,maintained
R3312	T5164	T5163	prep	as,late
R3313	T5165	T5164	pobj	E15.5,as
R3314	T5166	T5141	punct	", ",maintained
R3315	T5167	T5168	advmod	long,started
R3316	T5168	T5141	advcl	started,maintained
R3317	T5169	T5168	mark	after,started
R3318	T5170	T5168	nsubj	it,started
R3319	T5171	T5168	aux	has,started
R332	T1211	T1209	prep	of,variety
R3320	T5172	T5173	aux	to,degrade
R3321	T5173	T5168	xcomp	degrade,started
R3322	T5174	T5173	prep	in,degrade
R3323	T5175	T5176	amod	wild,type
R3324	T5176	T5178	compound	type,limbs
R3325	T5177	T5176	punct	-,type
R3326	T5178	T5174	pobj	limbs,in
R3327	T5179	T5141	punct	.,maintained
R3328	T5181	T5182	nsubjpass	This,seen
R3329	T5183	T5182	aux	can,seen
R333	T1212	T1213	amod	developmental,processes
R3330	T5184	T5182	auxpass	be,seen
R3331	T5185	T5186	advmod	morphologically,by
R3332	T5186	T5182	prep	by,seen
R3333	T5187	T5188	punct	(,data
R3334	T5188	T5185	meta	data,morphologically
R3335	T5189	T5188	amod	unpublished,data
R3336	T5190	T5188	punct	),data
R3337	T5191	T5186	cc	or,by
R3338	T5192	T5186	pcomp	staining,by
R3339	T5193	T5192	prep	for,staining
R334	T1213	T1211	pobj	processes,of
R3340	T5194	T5193	pobj	expression,for
R3341	T5195	T5194	prep	of,expression
R3342	T5196	T5197	nmod	AER,marker
R3343	T5197	T5195	pobj	marker,of
R3344	T5198	T5196	punct	-,AER
R3345	T5199	T5196	amod	specific,AER
R3346	T5200	T5201	amod	such,as
R3347	T5201	T5197	prep	as,marker
R3348	T5202	T5201	pobj	Fgf8,as
R3349	T5203	T5204	punct	(,2I
R335	T1214	T1215	punct	[,4
R3350	T5204	T5182	parataxis	2I,seen
R3351	T5205	T5204	compound	Figure,2I
R3352	T5206	T5204	cc	and,2I
R3353	T5207	T5204	conj	2J,2I
R3354	T5208	T5204	punct	),2I
R3355	T5209	T5182	punct	.,seen
R3356	T5211	T5212	det	The,AER
R3357	T5212	T5213	nsubj	AER,displays
R3358	T5214	T5215	amod	intense,staining
R3359	T5215	T5213	dobj	staining,displays
R336	T1215	T1196	parataxis	4,known
R3360	T5216	T5213	prep	with,displays
R3361	T5217	T5218	compound	acridine,orange
R3362	T5218	T5216	pobj	orange,with
R3363	T5219	T5218	punct	", ",orange
R3364	T5220	T5218	conj	TUNEL,orange
R3365	T5221	T5220	punct	", ",TUNEL
R3366	T5222	T5220	cc	or,TUNEL
R3367	T5223	T5224	amod	other,markers
R3368	T5224	T5220	conj	markers,TUNEL
R3369	T5225	T5224	prep	for,markers
R337	T1216	T1215	punct	],4
R3370	T5226	T5225	pobj	apoptosis,for
R3371	T5227	T5213	prep	at,displays
R3372	T5228	T5229	det	every,stage
R3373	T5229	T5227	pobj	stage,at
R3374	T5230	T5231	advmod	when,is
R3375	T5231	T5213	advcl	is,displays
R3376	T5232	T5231	nsubj	it,is
R3377	T5233	T5231	acomp	present,is
R3378	T5234	T5213	punct	", ",displays
R3379	T5235	T5213	advcl	reflecting,displays
R338	T1217	T1196	punct	.,known
R3380	T5236	T5237	det	the,turnover
R3381	T5237	T5235	dobj	turnover,reflecting
R3382	T5238	T5237	amod	rapid,turnover
R3383	T5239	T5237	prep	of,turnover
R3384	T5240	T5239	pobj	cells,of
R3385	T5241	T5237	prep	in,turnover
R3386	T5242	T5243	det	this,structure
R3387	T5243	T5241	pobj	structure,in
R3388	T5244	T5245	punct	[,33
R3389	T5245	T5213	parataxis	33,displays
R339	T1219	T1220	advmod	Even,in
R3390	T5246	T5247	punct	–,35
R3391	T5247	T5245	prep	35,33
R3392	T5248	T5245	punct	],33
R3393	T5249	T5213	punct	.,displays
R3394	T5251	T5252	det	The,limbs
R3395	T5252	T5266	nsubj	limbs,show
R3396	T5253	T5254	nmod	Bmp2C,C
R3397	T5254	T5256	nmod	C,mutant
R3398	T5255	T5254	punct	/,C
R3399	T5256	T5252	compound	mutant,limbs
R34	T191	T180	prep	throughout,expressed
R340	T1220	T1221	prep	in,play
R3400	T5257	T5254	punct	;,C
R3401	T5258	T5259	compound	Bmp4C,C
R3402	T5259	T5254	appos	C,C
R3403	T5260	T5259	punct	/,C
R3404	T5261	T5254	punct	;,C
R3405	T5262	T5263	compound	Prx1,cre
R3406	T5263	T5254	appos	cre,C
R3407	T5264	T5263	punct	:,cre
R3408	T5265	T5263	punct	:,cre
R3409	T5267	T5266	prep	at,show
R341	T1222	T1223	det	the,context
R3410	T5268	T5267	pobj	E15.5,at
R3411	T5269	T5270	amod	strong,staining
R3412	T5270	T5266	dobj	staining,show
R3413	T5271	T5272	compound	acridine,orange
R3414	T5272	T5270	compound	orange,staining
R3415	T5273	T5266	prep	in,show
R3416	T5274	T5275	det	the,remnants
R3417	T5275	T5273	pobj	remnants,in
R3418	T5276	T5275	prep	of,remnants
R3419	T5277	T5276	pobj	AER,of
R342	T1223	T1220	pobj	context,in
R3420	T5278	T5279	punct	(,asterisk
R3421	T5279	T5275	parataxis	asterisk,remnants
R3422	T5280	T5281	compound	Figure,2H
R3423	T5281	T5279	dep	2H,asterisk
R3424	T5282	T5279	punct	", ",asterisk
R3425	T5283	T5279	punct	),asterisk
R3426	T5284	T5266	punct	", ",show
R3427	T5285	T5266	prep	in,show
R3428	T5286	T5285	pobj	domains,in
R3429	T5287	T5286	prep	of,domains
R343	T1224	T1223	prep	of,context
R3430	T5288	T5289	det	the,tip
R3431	T5289	T5287	pobj	tip,of
R3432	T5290	T5289	amod	distal,tip
R3433	T5291	T5292	advmod	where,remains
R3434	T5292	T5286	advcl	remains,domains
R3435	T5293	T5294	det	the,AER
R3436	T5294	T5292	nsubj	AER,remains
R3437	T5295	T5296	advmod	the,longest
R3438	T5296	T5292	acomp	longest,remains
R3439	T5297	T5298	punct	(,compare
R344	T1225	T1226	det	the,morphogenesis
R3440	T5298	T5266	parataxis	compare,show
R3441	T5299	T5298	dobj	staining,compare
R3442	T5300	T5299	prep	in,staining
R3443	T5301	T5302	compound	Figure,2F
R3444	T5302	T5300	pobj	2F,in
R3445	T5303	T5302	cc	and,2F
R3446	T5304	T5302	conj	2J,2F
R3447	T5305	T5298	punct	),compare
R3448	T5306	T5266	punct	.,show
R3449	T5308	T5309	det	No,difference
R345	T1226	T1224	pobj	morphogenesis,of
R3450	T5309	T5311	nsubjpass	difference,observed
R3451	T5310	T5309	amod	significant,difference
R3452	T5312	T5311	auxpass	was,observed
R3453	T5313	T5311	prep	in,observed
R3454	T5314	T5315	det	the,extent
R3455	T5315	T5313	pobj	extent,in
R3456	T5316	T5315	prep	of,extent
R3457	T5317	T5316	pobj	apoptosis,of
R3458	T5318	T5317	prep	in,apoptosis
R3459	T5319	T5320	det	the,cells
R346	T1227	T1226	prep	of,morphogenesis
R3460	T5320	T5318	pobj	cells,in
R3461	T5321	T5320	prep	of,cells
R3462	T5322	T5323	det	the,AER
R3463	T5323	T5321	pobj	AER,of
R3464	T5324	T5323	prep	in,AER
R3465	T5325	T5326	amod	earlier,stage
R3466	T5326	T5327	nmod	stage,limbs
R3467	T5327	T5324	pobj	limbs,in
R3468	T5328	T5329	nmod	Bmp2C,C
R3469	T5329	T5331	nmod	C,mutant
R347	T1228	T1229	det	a,structure
R3470	T5330	T5329	punct	/,C
R3471	T5331	T5327	nmod	mutant,limbs
R3472	T5332	T5329	punct	;,C
R3473	T5333	T5334	compound	Bmp4C,C
R3474	T5334	T5329	appos	C,C
R3475	T5335	T5334	punct	/,C
R3476	T5336	T5329	punct	;,C
R3477	T5337	T5338	compound	Prx1,cre
R3478	T5338	T5329	appos	cre,C
R3479	T5339	T5338	punct	:,cre
R348	T1229	T1227	pobj	structure,of
R3480	T5340	T5338	punct	:,cre
R3481	T5341	T5327	amod	embryonic,limbs
R3482	T5342	T5343	punct	(,S1D
R3483	T5343	T5311	parataxis	S1D,observed
R3484	T5344	T5343	compound	Figure,S1D
R3485	T5345	T5346	punct	–,S1G
R3486	T5346	T5343	prep	S1G,S1D
R3487	T5347	T5343	punct	),S1D
R3488	T5348	T5311	punct	.,observed
R349	T1230	T1229	amod	single,structure
R3497	T5836	T5837	compound	Patterning,Defects
R3498	T5838	T5837	prep	in,Defects
R3499	T5839	T5838	pobj	Limb,in
R35	T192	T193	compound	limb,development
R350	T1231	T1221	punct	", ",play
R3500	T5840	T5839	acl	Developing,Limb
R3501	T5841	T5840	prep	with,Developing
R3502	T5842	T5843	amod	Reduced,Levels
R3503	T5843	T5841	pobj	Levels,with
R3504	T5844	T5843	prep	of,Levels
R3505	T5845	T5846	compound	BMP,Signaling
R3506	T5846	T5844	pobj	Signaling,of
R3507	T5848	T5849	compound	BMP,signaling
R3508	T5849	T5850	nsubjpass	signaling,proposed
R3509	T5851	T5850	aux	has,proposed
R351	T1232	T1233	det	these,molecules
R3510	T5852	T5850	auxpass	been,proposed
R3511	T5853	T5854	aux	to,play
R3512	T5854	T5850	xcomp	play,proposed
R3513	T5855	T5856	amod	several,roles
R3514	T5856	T5854	dobj	roles,play
R3515	T5857	T5856	amod	distinct,roles
R3516	T5858	T5854	prep	in,play
R3517	T5859	T5860	compound	limb,patterning
R3518	T5860	T5858	pobj	patterning,in
R3519	T5861	T5850	punct	.,proposed
R352	T1233	T1221	nsubj	molecules,play
R3520	T5863	T5864	det	The,first
R3521	T5864	T5865	nsubj	first,is
R3522	T5866	T5864	prep	of,first
R3523	T5867	T5866	pobj	these,of
R3524	T5868	T5865	prep	in,is
R3525	T5869	T5868	pcomp	establishing,in
R3526	T5870	T5869	dobj	differences,establishing
R3527	T5871	T5870	prep	along,differences
R3528	T5872	T5873	det	the,axis
R3529	T5873	T5871	pobj	axis,along
R353	T1234	T1221	aux	can,play
R3530	T5874	T5875	amod	anterior,posterior
R3531	T5875	T5873	amod	posterior,axis
R3532	T5876	T5875	punct	-,posterior
R3533	T5877	T5873	prep	of,axis
R3534	T5878	T5879	det	the,limb
R3535	T5879	T5877	pobj	limb,of
R3536	T5880	T5873	advmod	downstream,axis
R3537	T5881	T5880	prep	of,downstream
R3538	T5882	T5883	amod	sonic,hedgehog
R3539	T5883	T5881	pobj	hedgehog,of
R354	T1235	T1236	det	a,series
R3540	T5884	T5883	punct	(,hedgehog
R3541	T5885	T5883	appos	Shh,hedgehog
R3542	T5886	T5865	punct	),is
R3543	T5887	T5865	punct	.,is
R3544	T5889	T5890	compound	SHH,signaling
R3545	T5890	T5891	nsubj	signaling,extends
R3546	T5892	T5890	appos	itself,signaling
R3547	T5893	T5891	prep	over,extends
R3548	T5894	T5895	det	a,range
R3549	T5895	T5893	pobj	range,over
R355	T1236	T1221	dobj	series,play
R3550	T5896	T5895	amod	long,range
R3551	T5897	T5891	prep	in,extends
R3552	T5898	T5899	det	the,bud
R3553	T5899	T5897	pobj	bud,in
R3554	T5900	T5899	amod	developing,bud
R3555	T5901	T5899	compound	limb,bud
R3556	T5902	T5891	punct	", ",extends
R3557	T5903	T5891	prep	based,extends
R3558	T5904	T5903	prep	on,based
R3559	T5905	T5906	preconj	both,activation
R356	T1237	T1236	prep	of,series
R3560	T5906	T5904	pobj	activation,on
R3561	T5907	T5906	prep	of,activation
R3562	T5908	T5909	compound	target,genes
R3563	T5909	T5907	pobj	genes,of
R3564	T5910	T5911	punct	[,36
R3565	T5911	T5906	parataxis	36,activation
R3566	T5912	T5911	punct	],36
R3567	T5913	T5906	cc	and,activation
R3568	T5914	T5915	compound	protein,distribution
R3569	T5915	T5906	conj	distribution,activation
R357	T1238	T1239	advmod	quite,roles
R3570	T5916	T5917	punct	[,37
R3571	T5917	T5915	parataxis	37,distribution
R3572	T5918	T5917	punct	],37
R3573	T5919	T5891	punct	.,extends
R3574	T5921	T5922	advmod	Nonetheless,suggested
R3575	T5923	T5922	punct	", ",suggested
R3576	T5924	T5925	det	some,models
R3577	T5925	T5922	nsubj	models,suggested
R3578	T5926	T5922	aux	have,suggested
R3579	T5927	T5928	mark	that,mediated
R358	T1239	T1237	pobj	roles,of
R3580	T5928	T5922	ccomp	mediated,suggested
R3581	T5929	T5930	det	the,effect
R3582	T5930	T5928	nsubjpass	effect,mediated
R3583	T5931	T5930	compound	patterning,effect
R3584	T5932	T5930	prep	of,effect
R3585	T5933	T5934	det	this,signal
R3586	T5934	T5932	pobj	signal,of
R3587	T5935	T5934	amod	morphogenic,signal
R3588	T5936	T5928	auxpass	is,mediated
R3589	T5937	T5938	advmod	entirely,in
R359	T1240	T1239	amod	divergent,roles
R3590	T5938	T5928	prep	in,mediated
R3591	T5939	T5940	punct	[,10
R3592	T5940	T5937	parataxis	10,entirely
R3593	T5941	T5940	punct	],10
R3594	T5942	T5938	cc	or,in
R3595	T5943	T5938	amod	part,in
R3596	T5944	T5945	punct	[,11
R3597	T5945	T5938	parataxis	11,in
R3598	T5946	T5945	punct	],11
R3599	T5947	T5928	agent	by,mediated
R36	T193	T191	pobj	development,throughout
R360	T1241	T1221	punct	.,play
R3600	T5948	T5947	pobj	BMP2,by
R3601	T5949	T5948	cc	and,BMP2
R3602	T5950	T5948	conj	BMP7,BMP2
R3603	T5951	T5948	acl	produced,BMP2
R3604	T5952	T5951	prep	as,produced
R3605	T5953	T5954	amod	secondary,signals
R3606	T5954	T5952	pobj	signals,as
R3607	T5955	T5951	prep	in,produced
R3608	T5956	T5955	pobj	response,in
R3609	T5957	T5956	prep	to,response
R361	T1243	T1244	prep	For,postulated
R3610	T5958	T5957	pobj	SHH,to
R3611	T5959	T5922	punct	.,suggested
R3612	T5961	T5962	nsubj	We,removed
R3613	T5963	T5962	aux	have,removed
R3614	T5964	T5965	preconj	both,BMP2
R3615	T5965	T5966	nmod	BMP2,activities
R3616	T5966	T5962	dobj	activities,removed
R3617	T5967	T5965	cc	and,BMP2
R3618	T5968	T5965	conj	BMP7,BMP2
R3619	T5969	T5962	prep	in,removed
R362	T1245	T1243	pobj	example,For
R3620	T5970	T5971	det	the,bud
R3621	T5971	T5969	pobj	bud,in
R3622	T5972	T5971	amod	early,bud
R3623	T5973	T5971	compound	limb,bud
R3624	T5974	T5975	punct	(,Figure
R3625	T5975	T5962	parataxis	Figure,removed
R3626	T5976	T5975	nummod	1,Figure
R3627	T5977	T5975	punct	),Figure
R3628	T5978	T5962	punct	", ",removed
R3629	T5979	T5962	cc	and,removed
R363	T1246	T1244	punct	", ",postulated
R3630	T5980	T5981	advmod	moreover,is
R3631	T5981	T5962	conj	is,removed
R3632	T5982	T5981	punct	", ",is
R3633	T5983	T5981	expl	there,is
R3634	T5984	T5985	det	no,upregulation
R3635	T5985	T5981	attr	upregulation,is
R3636	T5986	T5985	amod	apparent,upregulation
R3637	T5987	T5985	prep	of,upregulation
R3638	T5988	T5989	compound	BMP4,expression
R3639	T5989	T5987	pobj	expression,of
R364	T1247	T1244	prep	during,postulated
R3640	T5990	T5985	prep	in,upregulation
R3641	T5991	T5992	det	the,posterior
R3642	T5992	T5990	pobj	posterior,in
R3643	T5993	T5992	prep	of,posterior
R3644	T5994	T5995	det	these,limbs
R3645	T5995	T5993	pobj	limbs,of
R3646	T5996	T5997	punct	(,3A
R3647	T5997	T5981	parataxis	3A,is
R3648	T5998	T5997	compound	Figure,3A
R3649	T5999	T6000	punct	–,3D
R365	T1248	T1249	compound	limb,development
R3650	T6000	T5997	prep	3D,3A
R3651	T6001	T5997	punct	),3A
R3652	T6002	T5981	punct	.,is
R3653	T6004	T6005	det	The,phenotypes
R3654	T6005	T6007	nsubj	phenotypes,formed
R3655	T6006	T6005	amod	skeletal,phenotypes
R3656	T6008	T6005	prep	of,phenotypes
R3657	T6009	T6010	compound	mouse,limbs
R3658	T6010	T6008	pobj	limbs,of
R3659	T6011	T6007	prep	in,formed
R366	T1249	T1247	pobj	development,during
R3660	T6012	T6013	det	the,absence
R3661	T6013	T6011	pobj	absence,in
R3662	T6014	T6013	prep	of,absence
R3663	T6015	T6016	nmod	BMP2,BMP7
R3664	T6016	T6014	pobj	BMP7,of
R3665	T6017	T6016	punct	", ",BMP7
R3666	T6018	T6007	punct	", ",formed
R3667	T6019	T6007	cc	and,formed
R3668	T6020	T6021	preconj	both,BMP2
R3669	T6021	T6022	nsubj	BMP2,are
R367	T1250	T1244	punct	", ",postulated
R3670	T6022	T6007	conj	are,formed
R3671	T6023	T6021	cc	and,BMP2
R3672	T6024	T6021	conj	BMP7,BMP2
R3673	T6025	T6022	acomp	illuminating,are
R3674	T6026	T6022	punct	", ",are
R3675	T6027	T6028	mark	as,exhibit
R3676	T6028	T6022	advcl	exhibit,are
R3677	T6029	T6028	nsubj	none,exhibit
R3678	T6030	T6029	prep	of,none
R3679	T6031	T6030	pobj	these,of
R368	T1251	T1244	nsubjpass	BMPs,postulated
R3680	T6032	T6033	amod	significant,defects
R3681	T6033	T6028	dobj	defects,exhibit
R3682	T6034	T6033	compound	digit,defects
R3683	T6035	T6033	compound	pattern,defects
R3684	T6036	T6037	punct	(,Figure
R3685	T6037	T6022	parataxis	Figure,are
R3686	T6038	T6037	nummod	1,Figure
R3687	T6039	T6037	punct	),Figure
R3688	T6040	T6022	punct	.,are
R3689	T6042	T6043	mark	While,is
R369	T1252	T1244	aux	have,postulated
R3690	T6043	T6047	advcl	is,is
R3691	T6044	T6045	det	a,phalanx
R3692	T6045	T6043	nsubj	phalanx,is
R3693	T6046	T6045	amod	distal,phalanx
R3694	T6048	T6043	acomp	missing,is
R3695	T6049	T6043	prep	in,is
R3696	T6050	T6051	det	the,digits
R3697	T6051	T6049	pobj	digits,in
R3698	T6052	T6051	amod	middle,digits
R3699	T6053	T6051	prep	of,digits
R37	T194	T180	punct	.,expressed
R370	T1253	T1244	auxpass	been,postulated
R3700	T6054	T6055	det	the,mutants
R3701	T6055	T6053	pobj	mutants,of
R3702	T6056	T6055	amod	double,mutants
R3703	T6057	T6055	punct	(,mutants
R3704	T6058	T6059	compound	Bmp2C,C
R3705	T6059	T6055	appos	C,mutants
R3706	T6060	T6059	punct	/,C
R3707	T6061	T6059	punct	", ",C
R3708	T6062	T6059	appos	Bmp7,C
R3709	T6063	T6062	punct	−,Bmp7
R371	T1254	T1255	aux	to,act
R3710	T6064	T6062	punct	/,Bmp7
R3711	T6065	T6062	punct	−,Bmp7
R3712	T6066	T6059	punct	;,C
R3713	T6067	T6068	compound	Prx1,cre
R3714	T6068	T6059	appos	cre,C
R3715	T6069	T6068	punct	:,cre
R3716	T6070	T6068	punct	:,cre
R3717	T6071	T6047	punct	),is
R3718	T6072	T6047	punct	", ",is
R3719	T6073	T6047	nsubj	this,is
R372	T1255	T1244	xcomp	act,postulated
R3720	T6074	T6047	neg	not,is
R3721	T6075	T6076	det	a,phenotype
R3722	T6076	T6047	attr	phenotype,is
R3723	T6077	T6076	amod	consistent,phenotype
R3724	T6078	T6077	prep	with,consistent
R3725	T6079	T6080	det	a,decrease
R3726	T6080	T6078	pobj	decrease,with
R3727	T6081	T6080	cc	or,decrease
R3728	T6082	T6080	conj	loss,decrease
R3729	T6083	T6080	prep	of,decrease
R373	T1256	T1255	advmod	sequentially,act
R3730	T6084	T6085	det	a,activity
R3731	T6085	T6083	pobj	activity,of
R3732	T6086	T6087	advmod	posteriorly,derived
R3733	T6087	T6085	amod	derived,activity
R3734	T6088	T6085	compound	polarizing,activity
R3735	T6089	T6047	punct	.,is
R3736	T6091	T6092	advmod	Indeed,forms
R3737	T6093	T6092	punct	", ",forms
R3738	T6094	T6095	det	the,phalanx
R3739	T6095	T6092	nsubj	phalanx,forms
R374	T1257	T1255	prep	in,act
R3740	T6096	T6095	amod	terminal,phalanx
R3741	T6097	T6092	prep	at,forms
R3742	T6098	T6099	det	the,tip
R3743	T6099	T6097	pobj	tip,at
R3744	T6100	T6099	amod	distal,tip
R3745	T6101	T6099	prep	of,tip
R3746	T6102	T6103	det	each,digit
R3747	T6103	T6101	pobj	digit,of
R3748	T6104	T6092	prep	by,forms
R3749	T6105	T6106	det	a,mechanism
R375	T1258	T1259	amod	multiple,aspects
R3750	T6106	T6104	pobj	mechanism,by
R3751	T6107	T6106	amod	distinct,mechanism
R3752	T6108	T6106	punct	", ",mechanism
R3753	T6109	T6110	neg	not,dependent
R3754	T6110	T6106	relcl	dependent,mechanism
R3755	T6111	T6110	prep	upon,dependent
R3756	T6112	T6113	amod	anterior,posterior
R3757	T6113	T6115	amod	posterior,patterning
R3758	T6114	T6113	punct	-,posterior
R3759	T6115	T6111	pobj	patterning,upon
R376	T1259	T1257	pobj	aspects,in
R3760	T6116	T6117	punct	[,38
R3761	T6117	T6092	parataxis	38,forms
R3762	T6118	T6117	punct	],38
R3763	T6119	T6092	punct	.,forms
R3764	T6121	T6122	det	These,data
R3765	T6122	T6123	nsubj	data,indicate
R3766	T6124	T6123	advmod	strongly,indicate
R3767	T6125	T6126	mark	that,are
R3768	T6126	T6123	ccomp	are,indicate
R3769	T6127	T6126	nsubj	BMP2,are
R377	T1260	T1259	amod	distinct,aspects
R3770	T6128	T6127	cc	and,BMP2
R3771	T6129	T6127	conj	BMP7,BMP2
R3772	T6130	T6126	neg	not,are
R3773	T6131	T6132	amod	required,signals
R3774	T6132	T6126	attr	signals,are
R3775	T6133	T6132	amod	secondary,signals
R3776	T6134	T6126	prep	for,are
R3777	T6135	T6136	npadvmod	SHH,mediated
R3778	T6136	T6138	amod	mediated,polarization
R3779	T6137	T6136	punct	-,mediated
R378	T1261	T1259	prep	of,aspects
R3780	T6138	T6134	pobj	polarization,for
R3781	T6139	T6138	prep	of,polarization
R3782	T6140	T6141	det	the,digits
R3783	T6141	T6139	pobj	digits,of
R3784	T6142	T6141	prep	along,digits
R3785	T6143	T6144	det	the,axis
R3786	T6144	T6142	pobj	axis,along
R3787	T6145	T6146	amod	anterior,posterior
R3788	T6146	T6144	amod	posterior,axis
R3789	T6147	T6146	punct	-,posterior
R379	T1262	T1261	pobj	patterning,of
R3790	T6148	T6123	punct	.,indicate
R3791	T6150	T6151	det	The,lack
R3792	T6151	T6152	nsubj	lack,argues
R3793	T6153	T6151	prep	of,lack
R3794	T6154	T6153	pobj	transformations,of
R3795	T6155	T6154	prep	in,transformations
R3796	T6156	T6157	compound	digit,identity
R3797	T6157	T6155	pobj	identity,in
R3798	T6158	T6157	prep	in,identity
R3799	T6159	T6160	det	the,skeletons
R38	T196	T197	nsubjpass	BMPs,implicated
R380	T1263	T1262	punct	", ",patterning
R3800	T6160	T6158	pobj	skeletons,in
R3801	T6161	T6160	compound	limb,skeletons
R3802	T6162	T6154	acl	formed,transformations
R3803	T6163	T6162	prep	in,formed
R3804	T6164	T6165	det	the,absence
R3805	T6165	T6163	pobj	absence,in
R3806	T6166	T6165	prep	of,absence
R3807	T6167	T6168	nmod	BMP2,activity
R3808	T6168	T6166	pobj	activity,of
R3809	T6169	T6167	cc	and,BMP2
R381	T1264	T1265	compound	cell,type
R3810	T6170	T6167	conj	BMP7,BMP2
R3811	T6171	T6152	advmod	also,argues
R3812	T6172	T6152	prep	against,argues
R3813	T6173	T6174	compound	BMP,signaling
R3814	T6174	T6175	nsubj	signaling,being
R3815	T6175	T6172	pcomp	being,against
R3816	T6176	T6177	det	the,signal
R3817	T6177	T6175	attr	signal,being
R3818	T6178	T6177	amod	critical,signal
R3819	T6179	T6177	amod	instructive,signal
R382	T1265	T1266	compound	type,specification
R3820	T6180	T6177	prep	from,signal
R3821	T6181	T6182	det	the,mesenchyme
R3822	T6182	T6180	pobj	mesenchyme,from
R3823	T6183	T6182	amod	interdigital,mesenchyme
R3824	T6184	T6177	prep	to,signal
R3825	T6185	T6186	det	the,digits
R3826	T6186	T6184	pobj	digits,to
R3827	T6187	T6186	amod	forming,digits
R3828	T6188	T6152	punct	.,argues
R3829	T6190	T6191	mark	While,lost
R383	T1266	T1262	conj	specification,patterning
R3830	T6191	T6196	advcl	lost,have
R3831	T6192	T6193	det	a,phalanx
R3832	T6193	T6191	nsubjpass	phalanx,lost
R3833	T6194	T6193	amod	distal,phalanx
R3834	T6195	T6191	auxpass	was,lost
R3835	T6197	T6191	prep	from,lost
R3836	T6198	T6199	det	the,digit
R3837	T6199	T6197	pobj	digit,from
R3838	T6200	T6199	amod	middle,digit
R3839	T6201	T6199	prep	of,digit
R384	T1267	T1266	punct	", ",specification
R3840	T6202	T6203	det	the,forelimbs
R3841	T6203	T6201	pobj	forelimbs,of
R3842	T6204	T6203	prep	of,forelimbs
R3843	T6205	T6206	det	these,animals
R3844	T6206	T6204	pobj	animals,of
R3845	T6207	T6196	punct	", ",have
R3846	T6208	T6209	det	these,digits
R3847	T6209	T6196	nsubj	digits,have
R3848	T6210	T6196	aux	do,have
R3849	T6211	T6196	neg	not,have
R385	T1268	T1266	cc	and,specification
R3850	T6212	T6196	advmod	otherwise,have
R3851	T6213	T6214	det	a,morphology
R3852	T6214	T6196	dobj	morphology,have
R3853	T6215	T6214	amod	consistent,morphology
R3854	T6216	T6215	prep	with,consistent
R3855	T6217	T6218	nsubjpass	their,transformed
R3856	T6218	T6216	pcomp	transformed,with
R3857	T6219	T6218	auxpass	being,transformed
R3858	T6220	T6218	advmod	anteriorly,transformed
R3859	T6221	T6218	prep	into,transformed
R386	T1269	T1266	conj	differentiation,specification
R3860	T6222	T6221	pobj	digit,into
R3861	T6223	T6222	nummod	1,digit
R3862	T6224	T6222	punct	(,digit
R3863	T6225	T6222	appos	thumb,digit
R3864	T6226	T6196	punct	),have
R3865	T6227	T6196	punct	.,have
R3866	T6229	T6230	advmod	Indeed,are
R3867	T6231	T6230	punct	", ",are
R3868	T6232	T6230	prep	in,are
R3869	T6233	T6234	det	the,hindlimbs
R387	T1270	T1269	prep	of,differentiation
R3870	T6234	T6232	pobj	hindlimbs,in
R3871	T6235	T6234	punct	", ",hindlimbs
R3872	T6236	T6237	advmod	where,is
R3873	T6237	T6234	relcl	is,hindlimbs
R3874	T6238	T6239	det	the,penetrance
R3875	T6239	T6237	nsubj	penetrance,is
R3876	T6240	T6239	prep	of,penetrance
R3877	T6241	T6242	det	the,phenotype
R3878	T6242	T6240	pobj	phenotype,of
R3879	T6243	T6237	acomp	variable,is
R388	T1271	T1272	amod	various,tissues
R3880	T6244	T6230	punct	", ",are
R3881	T6245	T6246	det	the,digits
R3882	T6246	T6230	nsubj	digits,are
R3883	T6247	T6246	nmod	mutant,digits
R3884	T6248	T6246	amod	middle,digits
R3885	T6249	T6230	advmod	otherwise,are
R3886	T6250	T6230	acomp	indistinguishable,are
R3887	T6251	T6250	prep	from,indistinguishable
R3888	T6252	T6253	amod	wild,type
R3889	T6253	T6251	pobj	type,from
R389	T1272	T1270	pobj	tissues,of
R3890	T6254	T6253	punct	-,type
R3891	T6255	T6230	punct	", ",are
R3892	T6256	T6230	advmod	other,are
R3893	T6257	T6256	prep	than,other
R3894	T6258	T6259	det	the,presence
R3895	T6259	T6257	pobj	presence,than
R3896	T6260	T6259	cc	or,presence
R3897	T6261	T6259	conj	absence,presence
R3898	T6262	T6259	prep	of,presence
R3899	T6263	T6264	det	this,element
R39	T198	T197	aux	have,implicated
R390	T1273	T1272	punct	", ",tissues
R3900	T6264	T6262	pobj	element,of
R3901	T6265	T6264	amod	variable,element
R3902	T6266	T6264	amod	skeletal,element
R3903	T6267	T6230	punct	.,are
R3904	T6269	T6270	prep	In,obtained
R3905	T6271	T6269	pobj	contrast,In
R3906	T6272	T6271	prep	to,contrast
R3907	T6273	T6274	det	the,roles
R3908	T6274	T6272	pobj	roles,to
R3909	T6275	T6274	amod	proposed,roles
R391	T1274	T1275	advmod	particularly,of
R3910	T6276	T6274	prep	for,roles
R3911	T6277	T6276	pobj	BMPs,for
R3912	T6278	T6274	prep	in,roles
R3913	T6279	T6280	amod	anterior,posterior
R3914	T6280	T6282	amod	posterior,patterning
R3915	T6281	T6280	punct	-,posterior
R3916	T6282	T6278	pobj	patterning,in
R3917	T6283	T6282	compound	digit,patterning
R3918	T6284	T6270	punct	", ",obtained
R3919	T6285	T6270	nsubj	we,obtained
R392	T1275	T1272	prep	of,tissues
R3920	T6286	T6287	amod	further,support
R3921	T6287	T6270	dobj	support,obtained
R3922	T6288	T6287	prep	for,support
R3923	T6289	T6290	det	the,idea
R3924	T6290	T6288	pobj	idea,for
R3925	T6291	T6292	advmod	well,accepted
R3926	T6292	T6290	amod	accepted,idea
R3927	T6293	T6292	punct	-,accepted
R3928	T6294	T6295	mark	that,is
R3929	T6295	T6290	acl	is,idea
R393	T1276	T1277	det	the,skeleton
R3930	T6296	T6297	det	a,level
R3931	T6297	T6295	nsubj	level,is
R3932	T6298	T6297	compound	threshold,level
R3933	T6299	T6297	prep	of,level
R3934	T6300	T6301	compound	BMP,signaling
R3935	T6301	T6299	pobj	signaling,of
R3936	T6302	T6295	acomp	necessary,is
R3937	T6303	T6295	advmod	interdigitally,is
R3938	T6304	T6305	mark	for,occur
R3939	T6305	T6295	advcl	occur,is
R394	T1277	T1275	pobj	skeleton,of
R3940	T6306	T6307	amod	programmed,death
R3941	T6307	T6305	nsubj	death,occur
R3942	T6308	T6307	compound	cell,death
R3943	T6309	T6305	aux	to,occur
R3944	T6310	T6270	punct	.,obtained
R3945	T6312	T6313	nsubj	We,observed
R3946	T6314	T6313	dobj	syndactyly,observed
R3947	T6315	T6314	prep	with,syndactyly
R3948	T6316	T6317	amod	variable,penetrance
R3949	T6317	T6315	pobj	penetrance,with
R395	T1278	T1244	punct	.,postulated
R3950	T6318	T6314	prep	in,syndactyly
R3951	T6319	T6318	pobj	limbs,in
R3952	T6320	T6319	acl	developing,limbs
R3953	T6321	T6320	prep	without,developing
R3954	T6322	T6323	compound	BMP2,activity
R3955	T6323	T6321	pobj	activity,without
R3956	T6324	T6325	punct	(,2B
R3957	T6325	T6314	parataxis	2B,syndactyly
R3958	T6326	T6325	compound	Figure,2B
R3959	T6327	T6325	punct	),2B
R396	T1280	T1281	det	The,step
R3960	T6328	T6314	punct	", ",syndactyly
R3961	T6329	T6314	cc	and,syndactyly
R3962	T6330	T6331	advmod	nearly,complete
R3963	T6331	T6332	amod	complete,syndactyly
R3964	T6332	T6314	conj	syndactyly,syndactyly
R3965	T6333	T6334	amod	soft,tissue
R3966	T6334	T6332	compound	tissue,syndactyly
R3967	T6335	T6332	prep	of,syndactyly
R3968	T6336	T6337	det	all,digits
R3969	T6337	T6335	pobj	digits,of
R397	T1281	T1283	nsubj	step,is
R3970	T6338	T6332	prep	in,syndactyly
R3971	T6339	T6338	pobj	limbs,in
R3972	T6340	T6339	amod	deficient,limbs
R3973	T6341	T6340	prep	in,deficient
R3974	T6342	T6343	preconj	both,BMP2
R3975	T6343	T6341	pobj	BMP2,in
R3976	T6344	T6343	cc	and,BMP2
R3977	T6345	T6343	conj	BMP4,BMP2
R3978	T6346	T6347	punct	(,compare
R3979	T6347	T6332	parataxis	compare,syndactyly
R398	T1282	T1281	amod	earliest,step
R3980	T6348	T6349	compound	Figure,2C
R3981	T6349	T6347	dobj	2C,compare
R3982	T6350	T6349	cc	and,2C
R3983	T6351	T6352	compound	Figure,2D
R3984	T6352	T6349	conj	2D,2C
R3985	T6353	T6347	punct	),compare
R3986	T6354	T6313	punct	.,observed
R3987	T6356	T6357	det	The,defect
R3988	T6357	T6361	nsubj	defect,was
R3989	T6358	T6357	nummod	one,defect
R399	T1284	T1281	prep	of,step
R3990	T6359	T6357	amod	dramatic,defect
R3991	T6360	T6357	compound	patterning,defect
R3992	T6362	T6363	nsubj	we,observed
R3993	T6363	T6357	advcl	observed,defect
R3994	T6364	T6363	prep	in,observed
R3995	T6365	T6366	poss	our,series
R3996	T6366	T6364	pobj	series,in
R3997	T6367	T6368	compound	BMP,deficiency
R3998	T6368	T6366	compound	deficiency,series
R3999	T6369	T6370	det	the,loss
R4	T158	T157	prep	of,Roles
R40	T199	T197	auxpass	been,implicated
R400	T1285	T1286	compound	limb,development
R4000	T6370	T6361	attr	loss,was
R4001	T6371	T6370	prep	of,loss
R4002	T6372	T6373	amod	posterior,digits
R4003	T6373	T6371	pobj	digits,of
R4004	T6374	T6370	prep	in,loss
R4005	T6375	T6374	pobj	forelimbs,in
R4006	T6376	T6375	amod	deficient,forelimbs
R4007	T6377	T6376	prep	in,deficient
R4008	T6378	T6379	preconj	both,BMP2
R4009	T6379	T6377	pobj	BMP2,in
R401	T1286	T1284	pobj	development,of
R4010	T6380	T6379	cc	and,BMP2
R4011	T6381	T6379	conj	BMP4,BMP2
R4012	T6382	T6383	punct	(,1K
R4013	T6383	T6370	parataxis	1K,loss
R4014	T6384	T6383	compound	Figure,1K
R4015	T6385	T6383	punct	),1K
R4016	T6386	T6370	punct	", ",loss
R4017	T6387	T6388	advmod	also,observed
R4018	T6388	T6370	acl	observed,loss
R4019	T6389	T6388	prep	in,observed
R402	T1287	T1288	prep	in,implicated
R4020	T6390	T6391	amod	earlier,buds
R4021	T6391	T6389	pobj	buds,in
R4022	T6392	T6391	compound	limb,buds
R4023	T6393	T6391	acl	stained,buds
R4024	T6394	T6395	aux	to,show
R4025	T6395	T6393	advcl	show,stained
R4026	T6396	T6397	det	no,expression
R4027	T6397	T6395	dobj	expression,show
R4028	T6398	T6397	prep	of,expression
R4029	T6399	T6400	det	the,Sox9
R403	T1288	T1281	relcl	implicated,step
R4030	T6400	T6398	pobj	Sox9,of
R4031	T6401	T6400	compound	condensation,Sox9
R4032	T6402	T6400	compound	marker,Sox9
R4033	T6403	T6404	punct	(,Figure
R4034	T6404	T6395	parataxis	Figure,show
R4035	T6405	T6404	nummod	3,Figure
R4036	T6406	T6404	punct	),Figure
R4037	T6407	T6361	punct	.,was
R4038	T6409	T6410	aux	To,understand
R4039	T6410	T6411	advcl	understand,analyzed
R404	T1289	T1287	pobj	which,in
R4040	T6412	T6413	det	this,phenotype
R4041	T6413	T6410	dobj	phenotype,understand
R4042	T6414	T6411	punct	", ",analyzed
R4043	T6415	T6411	nsubj	we,analyzed
R4044	T6416	T6417	compound	limb,buds
R4045	T6417	T6411	dobj	buds,analyzed
R4046	T6418	T6411	prep	at,analyzed
R4047	T6419	T6420	amod	various,stages
R4048	T6420	T6418	pobj	stages,at
R4049	T6421	T6411	advcl	using,analyzed
R405	T1290	T1291	compound	BMP,signaling
R4050	T6422	T6423	det	a,series
R4051	T6423	T6421	dobj	series,using
R4052	T6424	T6423	prep	of,series
R4053	T6425	T6426	amod	molecular,markers
R4054	T6426	T6424	pobj	markers,of
R4055	T6427	T6411	punct	.,analyzed
R4056	T6429	T6430	prep	By,are
R4057	T6431	T6429	pobj	E11.5,By
R4058	T6432	T6430	punct	", ",are
R4059	T6433	T6434	det	the,buds
R406	T1291	T1288	nsubjpass	signaling,implicated
R4060	T6434	T6430	nsubj	buds,are
R4061	T6435	T6436	nmod	Bmp2C,C
R4062	T6436	T6434	nmod	C,buds
R4063	T6437	T6436	punct	/,C
R4064	T6438	T6436	punct	;,C
R4065	T6439	T6440	compound	Bmp4C,C
R4066	T6440	T6436	appos	C,C
R4067	T6441	T6440	punct	/,C
R4068	T6442	T6436	punct	;,C
R4069	T6443	T6444	compound	Prx1,cre
R407	T1292	T1288	aux	has,implicated
R4070	T6444	T6436	appos	cre,C
R4071	T6445	T6444	punct	:,cre
R4072	T6446	T6444	punct	:,cre
R4073	T6447	T6434	compound	limb,buds
R4074	T6448	T6449	advmod	noticeably,broader
R4075	T6449	T6450	amod	broader,expanded
R4076	T6450	T6430	acomp	expanded,are
R4077	T6451	T6450	punct	", ",expanded
R4078	T6452	T6450	prep	in,expanded
R4079	T6453	T6454	det	the,posterior
R408	T1293	T1288	auxpass	been,implicated
R4080	T6454	T6452	pobj	posterior,in
R4081	T6455	T6430	advcl	relative,are
R4082	T6456	T6455	prep	to,relative
R4083	T6457	T6458	amod	wild,type
R4084	T6458	T6456	pobj	type,to
R4085	T6459	T6458	punct	-,type
R4086	T6460	T6430	punct	.,are
R4087	T6462	T6463	nsubj	This,correlates
R4088	T6464	T6463	prep	with,correlates
R4089	T6465	T6466	det	an,expansion
R409	T1294	T1295	det	the,establishment
R4090	T6466	T6464	pobj	expansion,with
R4091	T6467	T6466	amod	anterior,expansion
R4092	T6468	T6466	prep	in,expansion
R4093	T6469	T6470	compound	Shh,expression
R4094	T6470	T6468	pobj	expression,in
R4095	T6471	T6472	punct	(,bracket
R4096	T6472	T6466	parataxis	bracket,expansion
R4097	T6473	T6474	compound	Figure,3E
R4098	T6474	T6472	dep	3E,bracket
R4099	T6475	T6474	cc	and,3E
R41	T200	T197	prep	in,implicated
R410	T1295	T1283	attr	establishment,is
R4100	T6476	T6474	conj	3F,3E
R4101	T6477	T6472	punct	", ",bracket
R4102	T6478	T6472	amod	red,bracket
R4103	T6479	T6472	punct	),bracket
R4104	T6480	T6466	cc	and,expansion
R4105	T6481	T6482	det	a,expansion
R4106	T6482	T6466	conj	expansion,expansion
R4107	T6483	T6482	amod	concomitant,expansion
R4108	T6484	T6482	prep	of,expansion
R4109	T6485	T6486	det	the,AER
R411	T1296	T1295	prep	of,establishment
R4110	T6486	T6484	pobj	AER,of
R4111	T6487	T6488	mark	as,visualized
R4112	T6488	T6463	advcl	visualized,correlates
R4113	T6489	T6488	prep	by,visualized
R4114	T6490	T6491	compound	Fgf8,expression
R4115	T6491	T6489	pobj	expression,by
R4116	T6492	T6493	punct	(,bracket
R4117	T6493	T6463	parataxis	bracket,correlates
R4118	T6494	T6495	compound	Figure,3G
R4119	T6495	T6493	dep	3G,bracket
R412	T1297	T1298	det	the,axis
R4120	T6496	T6495	cc	and,3G
R4121	T6497	T6495	conj	3H,3G
R4122	T6498	T6493	punct	", ",bracket
R4123	T6499	T6493	amod	red,bracket
R4124	T6500	T6493	punct	),bracket
R4125	T6501	T6463	punct	.,correlates
R4126	T6503	T6504	det	The,expansion
R4127	T6504	T6505	nsubjpass	expansion,detected
R4128	T6506	T6504	prep	in,expansion
R4129	T6507	T6508	compound	Shh,expression
R413	T1298	T1296	pobj	axis,of
R4130	T6508	T6506	pobj	expression,in
R4131	T6509	T6505	aux	can,detected
R4132	T6510	T6505	advmod	first,detected
R4133	T6511	T6505	auxpass	be,detected
R4134	T6512	T6505	prep	at,detected
R4135	T6513	T6512	pobj	E10.5,at
R4136	T6514	T6505	cc	and,detected
R4137	T6515	T6505	conj	persists,detected
R4138	T6516	T6517	advmod	at,least
R4139	T6517	T6518	advmod	least,until
R414	T1299	T1300	amod	anterior,posterior
R4140	T6518	T6515	prep	until,persists
R4141	T6519	T6518	pobj	E12.5,until
R4142	T6520	T6515	punct	", ",persists
R4143	T6521	T6522	advmod	well,after
R4144	T6522	T6515	prep	after,persists
R4145	T6523	T6524	det	the,time
R4146	T6524	T6522	pobj	time,after
R4147	T6525	T6526	amod	normal,expression
R4148	T6526	T6528	nsubj	expression,disappears
R4149	T6527	T6526	compound	Shh,expression
R415	T1300	T1298	amod	posterior,axis
R4150	T6528	T6524	advcl	disappears,time
R4151	T6529	T6528	prep	at,disappears
R4152	T6530	T6529	pobj	E11.75,at
R4153	T6531	T6532	punct	(,3L
R4154	T6532	T6515	parataxis	3L,persists
R4155	T6533	T6532	compound	Figure,3L
R4156	T6534	T6532	cc	and,3L
R4157	T6535	T6532	conj	3P,3L
R4158	T6536	T6532	punct	),3L
R4159	T6537	T6505	punct	.,detected
R416	T1301	T1300	punct	-,posterior
R4160	T6539	T6540	det	The,expansion
R4161	T6540	T6541	nsubjpass	expansion,understood
R4162	T6542	T6540	prep	of,expansion
R4163	T6543	T6544	det	the,AER
R4164	T6544	T6542	pobj	AER,of
R4165	T6545	T6540	prep	in,expansion
R4166	T6546	T6547	det	the,absence
R4167	T6547	T6545	pobj	absence,in
R4168	T6548	T6547	prep	of,absence
R4169	T6549	T6550	compound	BMP,signaling
R417	T1302	T1298	compound	limb,axis
R4170	T6550	T6548	pobj	signaling,of
R4171	T6551	T6541	aux	may,understood
R4172	T6552	T6541	auxpass	be,understood
R4173	T6553	T6541	prep	on,understood
R4174	T6554	T6555	det	the,basis
R4175	T6555	T6553	pobj	basis,on
R4176	T6556	T6555	prep	of,basis
R4177	T6557	T6558	det	the,loop
R4178	T6558	T6556	pobj	loop,of
R4179	T6559	T6558	compound	feedback,loop
R418	T1303	T1283	punct	.,is
R4180	T6560	T6558	prep	between,loop
R4181	T6561	T6562	det	the,zone
R4182	T6562	T6560	pobj	zone,between
R4183	T6563	T6562	prep	of,zone
R4184	T6564	T6565	compound	polarizing,activity
R4185	T6565	T6563	pobj	activity,of
R4186	T6566	T6567	punct	(,ZPA
R4187	T6567	T6562	parataxis	ZPA,zone
R4188	T6568	T6567	punct	),ZPA
R4189	T6569	T6562	cc	and,zone
R419	T1305	T1306	nsubjpass	Differences,instructed
R4190	T6570	T6571	det	the,AER
R4191	T6571	T6562	conj	AER,zone
R4192	T6572	T6541	punct	.,understood
R4193	T6574	T6575	nsubj	Expression,depend
R4194	T6576	T6574	prep	of,Expression
R4195	T6577	T6578	amod	several,genes
R4196	T6578	T6576	pobj	genes,of
R4197	T6579	T6578	compound	Fgf,genes
R4198	T6580	T6581	punct	[,21
R4199	T6581	T6574	parataxis	21,Expression
R42	T201	T202	amod	early,patterning
R420	T1307	T1305	prep	in,Differences
R4200	T6582	T6581	nummod	20,21
R4201	T6583	T6581	punct	",",21
R4202	T6584	T6581	punct	],21
R4203	T6585	T6574	cc	and,Expression
R4204	T6586	T6574	conj	maintenance,Expression
R4205	T6587	T6586	prep	of,maintenance
R4206	T6588	T6589	det	the,AER
R4207	T6589	T6587	pobj	AER,of
R4208	T6590	T6589	appos	itself,AER
R4209	T6591	T6592	punct	[,23
R421	T1308	T1309	amod	anterior,posterior
R4210	T6592	T6586	parataxis	23,maintenance
R4211	T6593	T6592	punct	],23
R4212	T6594	T6575	advmod	normally,depend
R4213	T6595	T6575	prep	on,depend
R4214	T6596	T6597	compound	BMP,antagonism
R4215	T6597	T6595	pobj	antagonism,on
R4216	T6598	T6597	acl	induced,antagonism
R4217	T6599	T6598	agent	by,induced
R4218	T6600	T6599	pobj	SHH,by
R4219	T6601	T6575	punct	.,depend
R422	T1309	T1311	amod	posterior,pattern
R4220	T6603	T6604	advmod	Thus,expands
R4221	T6605	T6604	punct	", ",expands
R4222	T6606	T6607	det	a,decrease
R4223	T6607	T6604	nsubj	decrease,expands
R4224	T6608	T6607	prep	in,decrease
R4225	T6609	T6610	compound	BMP,signaling
R4226	T6610	T6608	pobj	signaling,in
R4227	T6611	T6604	cc	and,expands
R4228	T6612	T6604	conj	maintains,expands
R4229	T6613	T6614	det	the,AER
R423	T1310	T1309	punct	-,posterior
R4230	T6614	T6612	dobj	AER,maintains
R4231	T6615	T6604	punct	.,expands
R4232	T6617	T6618	mark	Because,supported
R4233	T6618	T6625	advcl	supported,feeds
R4234	T6619	T6620	compound	Shh,expression
R4235	T6620	T6618	nsubjpass	expression,supported
R4236	T6621	T6618	auxpass	is,supported
R4237	T6622	T6618	punct	", ",supported
R4238	T6623	T6618	advmod	reciprocally,supported
R4239	T6624	T6618	punct	", ",supported
R424	T1311	T1307	pobj	pattern,in
R4240	T6626	T6618	agent	by,supported
R4241	T6627	T6628	compound	FGF,signaling
R4242	T6628	T6626	pobj	signaling,by
R4243	T6629	T6628	prep	from,signaling
R4244	T6630	T6631	det	the,AER
R4245	T6631	T6629	pobj	AER,from
R4246	T6632	T6633	punct	[,24
R4247	T6633	T6618	parataxis	24,supported
R4248	T6634	T6633	nummod	8,24
R4249	T6635	T6633	punct	",",24
R425	T1312	T1306	auxpass	are,instructed
R4250	T6636	T6633	punct	],24
R4251	T6637	T6625	punct	", ",feeds
R4252	T6638	T6625	nsubj	this,feeds
R4253	T6639	T6625	punct	", ",feeds
R4254	T6640	T6625	prep	in,feeds
R4255	T6641	T6640	pobj	turn,in
R4256	T6642	T6625	punct	", ",feeds
R4257	T6643	T6625	prt	back,feeds
R4258	T6644	T6625	prep	on,feeds
R4259	T6645	T6646	det	the,ZPA
R426	T1313	T1306	prep	as,instructed
R4260	T6646	T6644	pobj	ZPA,on
R4261	T6647	T6625	punct	", ",feeds
R4262	T6648	T6625	advcl	expanding,feeds
R4263	T6649	T6650	compound	Shh,expression
R4264	T6650	T6648	dobj	expression,expanding
R4265	T6651	T6625	punct	.,feeds
R4266	T6653	T6654	mark	While,explains
R4267	T6654	T6656	advcl	explains,is
R4268	T6655	T6654	nsubj	this,explains
R4269	T6657	T6658	det	the,plate
R427	T1314	T1315	det	a,response
R4270	T6658	T6654	dobj	plate,explains
R4271	T6659	T6658	amod	broader,plate
R4272	T6660	T6658	compound	hand,plate
R4273	T6661	T6658	prep	of,plate
R4274	T6662	T6663	det	the,buds
R4275	T6663	T6661	pobj	buds,of
R4276	T6664	T6665	nmod	BMP2,BMP4
R4277	T6665	T6667	npadvmod	BMP4,deficient
R4278	T6666	T6665	punct	", ",BMP4
R4279	T6667	T6663	amod	deficient,buds
R428	T1315	T1313	pobj	response,as
R4280	T6668	T6667	punct	–,deficient
R4281	T6669	T6663	compound	limb,buds
R4282	T6670	T6656	punct	", ",is
R4283	T6671	T6656	nsubj	it,is
R4284	T6672	T6673	advmod	somewhat,paradoxical
R4285	T6673	T6656	acomp	paradoxical,is
R4286	T6674	T6656	prep	in,is
R4287	T6675	T6674	pobj	terms,in
R4288	T6676	T6675	prep	of,terms
R4289	T6677	T6678	det	the,phenotype
R429	T1316	T1315	amod	graded,response
R4290	T6678	T6676	pobj	phenotype,of
R4291	T6679	T6678	amod	skeletal,phenotype
R4292	T6680	T6656	punct	", ",is
R4293	T6681	T6682	mark	as,expected
R4294	T6682	T6656	advcl	expected,is
R4295	T6683	T6684	det	a,autopod
R4296	T6684	T6682	nsubjpass	autopod,expected
R4297	T6685	T6684	amod	broadened,autopod
R4298	T6686	T6682	aux	would,expected
R4299	T6687	T6682	advmod	normally,expected
R43	T202	T200	pobj	patterning,in
R430	T1317	T1315	prep	to,response
R4300	T6688	T6682	auxpass	be,expected
R4301	T6689	T6690	aux	to,result
R4302	T6690	T6682	xcomp	result,expected
R4303	T6691	T6690	prep	in,result
R4304	T6692	T6693	det	the,formation
R4305	T6693	T6691	pobj	formation,in
R4306	T6694	T6693	prep	of,formation
R4307	T6695	T6696	amod	extra,digits
R4308	T6696	T6694	pobj	digits,of
R4309	T6697	T6693	punct	(,formation
R431	T1318	T1319	amod	Sonic,Hedghog
R4310	T6698	T6693	appos	polydactyly,formation
R4311	T6699	T6693	punct	),formation
R4312	T6700	T6701	punct	(,1G
R4313	T6701	T6693	parataxis	1G,formation
R4314	T6702	T6701	advmod	e.g.,1G
R4315	T6703	T6701	punct	", ",1G
R4316	T6704	T6701	compound	Figure,1G
R4317	T6705	T6701	cc	and,1G
R4318	T6706	T6701	conj	1O,1G
R4319	T6707	T6701	prep	of,1G
R432	T1319	T1320	nmod	Hedghog,signaling
R4320	T6708	T6709	det	this,paper
R4321	T6709	T6707	pobj	paper,of
R4322	T6710	T6701	cc	and,1G
R4323	T6711	T6712	punct	[,16
R4324	T6712	T6701	conj	16,1G
R4325	T6713	T6701	punct	],1G
R4326	T6714	T6701	punct	),1G
R4327	T6715	T6693	punct	", ",formation
R4328	T6716	T6693	cc	not,formation
R4329	T6717	T6718	det	the,loss
R433	T1320	T1317	pobj	signaling,to
R4330	T6718	T6693	conj	loss,formation
R4331	T6719	T6718	prep	of,loss
R4332	T6720	T6719	pobj	digits,of
R4333	T6721	T6690	punct	", ",result
R4334	T6722	T6723	mark	as,observed
R4335	T6723	T6690	advcl	observed,result
R4336	T6724	T6723	prep	in,observed
R4337	T6725	T6726	det	the,forelimbs
R4338	T6726	T6724	pobj	forelimbs,in
R4339	T6727	T6728	amod	double,mutant
R434	T1321	T1319	punct	(,Hedghog
R4340	T6728	T6726	compound	mutant,forelimbs
R4341	T6729	T6656	punct	.,is
R4342	T6731	T6732	aux	To,examine
R4343	T6732	T6733	advcl	examine,examined
R4344	T6734	T6735	det	the,loss
R4345	T6735	T6732	dobj	loss,examine
R4346	T6736	T6735	prep	of,loss
R4347	T6737	T6736	pobj	digits,of
R4348	T6738	T6739	advmod	more,closely
R4349	T6739	T6732	advmod	closely,examine
R435	T1322	T1319	appos	SHH,Hedghog
R4350	T6740	T6733	punct	", ",examined
R4351	T6741	T6742	nmod	BMP2,BMP4
R4352	T6742	T6744	npadvmod	BMP4,deficient
R4353	T6743	T6742	punct	", ",BMP4
R4354	T6744	T6746	amod	deficient,buds
R4355	T6745	T6744	punct	–,deficient
R4356	T6746	T6733	nsubjpass	buds,examined
R4357	T6747	T6746	compound	limb,buds
R4358	T6748	T6733	auxpass	were,examined
R4359	T6749	T6733	prep	during,examined
R436	T1323	T1320	punct	),signaling
R4360	T6750	T6751	compound	cartilage,phase
R4361	T6751	T6749	pobj	phase,during
R4362	T6752	T6751	compound	condensation,phase
R4363	T6753	T6751	prep	of,phase
R4364	T6754	T6755	det	the,rays
R4365	T6755	T6753	pobj	rays,of
R4366	T6756	T6755	compound	digit,rays
R4367	T6757	T6733	prep	at,examined
R4368	T6758	T6757	pobj	E12.5,at
R4369	T6759	T6733	punct	.,examined
R437	T1324	T1320	acl	emanating,signaling
R4370	T6761	T6762	prep	In,visualized
R4371	T6763	T6764	amod	wild,type
R4372	T6764	T6766	compound	type,buds
R4373	T6765	T6764	punct	-,type
R4374	T6766	T6761	pobj	buds,In
R4375	T6767	T6766	compound	limb,buds
R4376	T6768	T6769	det	the,condensation
R4377	T6769	T6762	nsubjpass	condensation,visualized
R4378	T6770	T6769	prep	of,condensation
R4379	T6771	T6772	det	all,digits
R438	T1325	T1324	prep	from,emanating
R4380	T6772	T6770	pobj	digits,of
R4381	T6773	T6772	nummod	five,digits
R4382	T6774	T6762	aux	can,visualized
R4383	T6775	T6762	auxpass	be,visualized
R4384	T6776	T6762	prep	at,visualized
R4385	T6777	T6778	det	this,stage
R4386	T6778	T6776	pobj	stage,at
R4387	T6779	T6762	prep	by,visualized
R4388	T6780	T6781	advmod	in,situ
R4389	T6781	T6782	amod	situ,hybridization
R439	T1326	T1327	det	the,margin
R4390	T6782	T6779	pobj	hybridization,by
R4391	T6783	T6762	prep	with,visualized
R4392	T6784	T6785	det	a,probe
R4393	T6785	T6783	pobj	probe,with
R4394	T6786	T6785	acl	directed,probe
R4395	T6787	T6786	prep	against,directed
R4396	T6788	T6787	pobj	Sox9,against
R4397	T6789	T6788	punct	", ",Sox9
R4398	T6790	T6791	det	the,marker
R4399	T6791	T6788	appos	marker,Sox9
R44	T203	T202	compound	limb,patterning
R440	T1327	T1325	pobj	margin,from
R4400	T6792	T6791	amod	earliest,marker
R4401	T6793	T6791	amod	known,marker
R4402	T6794	T6791	amod	chondrogenic,marker
R4403	T6795	T6796	punct	(,3I
R4404	T6796	T6762	parataxis	3I,visualized
R4405	T6797	T6796	compound	Figure,3I
R4406	T6798	T6796	punct	),3I
R4407	T6799	T6762	punct	.,visualized
R4408	T6801	T6802	advmod	However,detects
R4409	T6803	T6802	punct	", ",detects
R441	T1328	T1327	amod	posterior,margin
R4410	T6804	T6802	prep	in,detects
R4411	T6805	T6806	nmod	Bmp2C,C
R4412	T6806	T6808	nmod	C,buds
R4413	T6807	T6806	punct	/,C
R4414	T6808	T6804	pobj	buds,in
R4415	T6809	T6806	punct	;,C
R4416	T6810	T6811	compound	Bmp4C,C
R4417	T6811	T6806	appos	C,C
R4418	T6812	T6811	punct	/,C
R4419	T6813	T6806	punct	;,C
R442	T1329	T1327	prep	of,margin
R4420	T6814	T6815	compound	Prx1,cre
R4421	T6815	T6806	appos	cre,C
R4422	T6816	T6815	punct	:,cre
R4423	T6817	T6815	punct	:,cre
R4424	T6818	T6808	compound	limb,buds
R4425	T6819	T6808	prep	at,buds
R4426	T6820	T6819	pobj	E12.5,at
R4427	T6821	T6802	punct	", ",detects
R4428	T6822	T6802	nsubj	Sox9,detects
R4429	T6823	T6802	advmod	only,detects
R443	T1330	T1331	det	the,bud
R4430	T6824	T6802	dobj	primordia,detects
R4431	T6825	T6824	prep	of,primordia
R4432	T6826	T6827	det	the,digits
R4433	T6827	T6825	pobj	digits,of
R4434	T6828	T6827	amod	anterior,digits
R4435	T6829	T6827	nummod	three,digits
R4436	T6830	T6827	prep	of,digits
R4437	T6831	T6832	det	the,forelimb
R4438	T6832	T6830	pobj	forelimb,of
R4439	T6833	T6802	punct	", ",detects
R444	T1331	T1329	pobj	bud,of
R4440	T6834	T6802	advcl	indicating,detects
R4441	T6835	T6836	det	a,failure
R4442	T6836	T6834	dobj	failure,indicating
R4443	T6837	T6836	prep	in,failure
R4444	T6838	T6839	det	the,formation
R4445	T6839	T6837	pobj	formation,in
R4446	T6840	T6839	prep	of,formation
R4447	T6841	T6842	amod	posterior,digits
R4448	T6842	T6840	pobj	digits,of
R4449	T6843	T6834	prep	at,indicating
R445	T1332	T1331	compound	limb,bud
R4450	T6844	T6845	det	this,stage
R4451	T6845	T6843	pobj	stage,at
R4452	T6846	T6845	amod	earliest,stage
R4453	T6847	T6848	punct	(,3J
R4454	T6848	T6802	parataxis	3J,detects
R4455	T6849	T6848	compound	Figure,3J
R4456	T6850	T6848	punct	),3J
R4457	T6851	T6802	punct	.,detects
R4458	T6853	T6854	nsubj	Genes,continue
R4459	T6855	T6856	amod	such,as
R446	T1333	T1334	punct	[,5
R4460	T6856	T6853	prep	as,Genes
R4461	T6857	T6856	pobj	Msx2,as
R4462	T6858	T6859	punct	(,3N
R4463	T6859	T6853	parataxis	3N,Genes
R4464	T6860	T6859	compound	Figure,3N
R4465	T6861	T6859	punct	),3N
R4466	T6862	T6863	aux	to,expressed
R4467	T6863	T6854	xcomp	expressed,continue
R4468	T6864	T6863	auxpass	be,expressed
R4469	T6865	T6863	prep	throughout,expressed
R447	T1334	T1306	parataxis	5,instructed
R4470	T6866	T6867	det	the,mesenchyme
R4471	T6867	T6865	pobj	mesenchyme,throughout
R4472	T6868	T6867	amod	posterior,mesenchyme
R4473	T6869	T6867	compound	forelimb,mesenchyme
R4474	T6870	T6863	punct	", ",expressed
R4475	T6871	T6863	advcl	indicating,expressed
R4476	T6872	T6873	mark	that,is
R4477	T6873	T6871	ccomp	is,indicating
R4478	T6874	T6875	det	the,tissue
R4479	T6875	T6873	nsubj	tissue,is
R448	T1335	T1334	punct	],5
R4480	T6876	T6877	amod	posterior,forelimb
R4481	T6877	T6875	compound	forelimb,tissue
R4482	T6878	T6873	acomp	viable,is
R4483	T6879	T6873	prep	at,is
R4484	T6880	T6879	pobj	E12.5,at
R4485	T6881	T6873	prep	in,is
R4486	T6882	T6883	det	the,animals
R4487	T6883	T6881	pobj	animals,in
R4488	T6884	T6885	nmod	Bmp2C,C
R4489	T6885	T6883	nmod	C,animals
R449	T1336	T1306	punct	.,instructed
R4490	T6886	T6885	punct	/,C
R4491	T6887	T6885	punct	;,C
R4492	T6888	T6889	compound	Bmp4C,C
R4493	T6889	T6885	appos	C,C
R4494	T6890	T6889	punct	/,C
R4495	T6891	T6885	punct	;,C
R4496	T6892	T6893	compound	Prx1,cre
R4497	T6893	T6885	appos	cre,C
R4498	T6894	T6893	punct	:,cre
R4499	T6895	T6893	punct	:,cre
R45	T204	T205	advmod	as,as
R450	T1338	T1339	nsubj	It,remained
R4500	T6896	T6854	punct	.,continue
R4501	T6898	T6899	nsubjpass	Msx2,expressed
R4502	T6900	T6899	auxpass	is,expressed
R4503	T6901	T6899	advmod	normally,expressed
R4504	T6902	T6899	prep	in,expressed
R4505	T6903	T6904	det	the,mesenchyme
R4506	T6904	T6902	pobj	mesenchyme,in
R4507	T6905	T6904	amod	non-chondrogenic,mesenchyme
R4508	T6906	T6904	amod	interdigital,mesenchyme
R4509	T6907	T6899	prep	at,expressed
R451	T1340	T1339	aux	has,remained
R4510	T6908	T6909	det	this,stage
R4511	T6909	T6907	pobj	stage,at
R4512	T6910	T6911	punct	(,3M
R4513	T6911	T6899	parataxis	3M,expressed
R4514	T6912	T6911	compound	Figure,3M
R4515	T6913	T6911	punct	),3M
R4516	T6914	T6899	punct	", ",expressed
R4517	T6915	T6916	det	a,continuation
R4518	T6916	T6899	npadvmod	continuation,expressed
R4519	T6917	T6916	prep	of,continuation
R452	T1341	T1339	oprd	controversial,remained
R4520	T6918	T6919	det	an,domain
R4521	T6919	T6917	pobj	domain,of
R4522	T6920	T6919	amod	earlier,domain
R4523	T6921	T6919	compound	expression,domain
R4524	T6922	T6916	prep	throughout,continuation
R4525	T6923	T6924	det	the,mesenchyme
R4526	T6924	T6922	pobj	mesenchyme,throughout
R4527	T6925	T6924	amod	distal,mesenchyme
R4528	T6926	T6899	punct	.,expressed
R4529	T6928	T6929	det	This,expression
R453	T1342	T1339	punct	", ",remained
R4530	T6929	T6930	nsubj	expression,reflects
R4531	T6931	T6929	prep	of,expression
R4532	T6932	T6931	pobj	Msx2,of
R4533	T6933	T6929	prep	in,expression
R4534	T6934	T6935	det	the,posterior
R4535	T6935	T6933	pobj	posterior,in
R4536	T6936	T6935	prep	of,posterior
R4537	T6937	T6938	det	the,forelimbs
R4538	T6938	T6936	pobj	forelimbs,of
R4539	T6939	T6940	nmod	BMP2,BMP4
R454	T1343	T1339	advmod	however,remained
R4540	T6940	T6942	npadvmod	BMP4,deficient
R4541	T6941	T6940	punct	", ",BMP4
R4542	T6942	T6938	amod	deficient,forelimbs
R4543	T6943	T6942	punct	–,deficient
R4544	T6944	T6945	det	a,failure
R4545	T6945	T6930	dobj	failure,reflects
R4546	T6946	T6945	prep	of,failure
R4547	T6947	T6946	pobj	chondrogenesis,of
R4548	T6948	T6945	prep	in,failure
R4549	T6949	T6950	det	this,region
R455	T1344	T1339	punct	", ",remained
R4550	T6950	T6948	pobj	region,in
R4551	T6951	T6930	punct	.,reflects
R4552	T6953	T6954	det	The,loss
R4553	T6954	T6955	nsubj	loss,appear
R4554	T6956	T6954	prep	of,loss
R4555	T6957	T6958	det	the,digits
R4556	T6958	T6956	pobj	digits,of
R4557	T6959	T6958	amod	posterior,digits
R4558	T6960	T6958	compound	forelimb,digits
R4559	T6961	T6954	prep	in,loss
R456	T1345	T1346	mark	whether,is
R4560	T6962	T6963	det	these,mice
R4561	T6963	T6961	pobj	mice,in
R4562	T6964	T6955	advmod	thus,appear
R4563	T6965	T6955	aux	does,appear
R4564	T6966	T6955	neg	not,appear
R4565	T6967	T6968	aux	to,represent
R4566	T6968	T6955	xcomp	represent,appear
R4567	T6969	T6970	det	a,decrease
R4568	T6970	T6968	dobj	decrease,represent
R4569	T6971	T6970	prep	in,decrease
R457	T1346	T1339	ccomp	is,remained
R4570	T6972	T6973	amod	polarizing,activity
R4571	T6973	T6971	pobj	activity,in
R4572	T6974	T6970	acl	leading,decrease
R4573	T6975	T6974	prep	to,leading
R4574	T6976	T6977	det	a,change
R4575	T6977	T6975	pobj	change,to
R4576	T6978	T6977	prep	in,change
R4577	T6979	T6980	compound	digit,identity
R4578	T6980	T6978	pobj	identity,in
R4579	T6981	T6982	advmod	rather,consequence
R458	T1347	T1348	det	this,response
R4580	T6982	T6970	appos	consequence,decrease
R4581	T6983	T6982	det	a,consequence
R4582	T6984	T6982	prep	of,consequence
R4583	T6985	T6986	det	the,level
R4584	T6986	T6988	nsubj	level,falling
R4585	T6987	T6986	amod	overall,level
R4586	T6988	T6984	pcomp	falling,of
R4587	T6989	T6986	prep	of,level
R4588	T6990	T6991	compound	BMP,signaling
R4589	T6991	T6989	pobj	signaling,of
R459	T1348	T1346	nsubj	response,is
R4590	T6992	T6988	prep	below,falling
R4591	T6993	T6994	det	a,threshold
R4592	T6994	T6992	pobj	threshold,below
R4593	T6995	T6994	prep	for,threshold
R4594	T6996	T6995	pcomp	initiating,for
R4595	T6997	T6996	dobj	chondrogenesis,initiating
R4596	T6998	T6996	prep	in,initiating
R4597	T6999	T7000	det	the,posterior
R4598	T7000	T6998	pobj	posterior,in
R4599	T7001	T7000	prep	of,posterior
R46	T205	T200	cc	as,in
R460	T1349	T1346	acomp	direct,is
R4600	T7002	T7003	det	these,forelimbs
R4601	T7003	T7001	pobj	forelimbs,of
R4602	T7004	T6955	punct	.,appear
R4603	T7006	T7007	det	The,posterior
R4604	T7007	T7008	nsubj	posterior,appears
R4605	T7009	T7007	prep	of,posterior
R4606	T7010	T7011	det	the,hindlimb
R4607	T7011	T7009	pobj	hindlimb,of
R4608	T7012	T7008	prep	in,appears
R4609	T7013	T7014	det	the,mutant
R461	T1350	T1349	cc	or,direct
R4610	T7014	T7012	pobj	mutant,in
R4611	T7015	T7008	punct	", ",appears
R4612	T7016	T7008	prep	in,appears
R4613	T7017	T7016	pobj	contrast,in
R4614	T7033	T7034	det	the,bud
R4615	T7018	T7008	punct	", ",appears
R4616	T7019	T7020	aux	to,remain
R4617	T7034	T7032	pobj	bud,in
R4618	T7035	T7034	compound	hindlimb,bud
R4619	T7020	T7008	xcomp	remain,appears
R462	T1351	T1349	conj	indirect,direct
R4620	T7036	T7037	punct	(,3K
R4621	T7037	T7031	parataxis	3K,form
R4622	T7021	T7020	prep	above,remain
R4623	T7038	T7037	compound	Figures,3K
R4624	T7039	T7037	cc	and,3K
R4625	T7040	T7037	conj	1S,3K
R4626	T7022	T7023	det	this,threshold
R4627	T7041	T7037	punct	),3K
R4628	T7042	T7031	cc	and,form
R4629	T7023	T7021	pobj	threshold,above
R463	T1352	T1339	punct	.,remained
R4630	T7043	T7044	det	no,expression
R4631	T7044	T7046	nsubjpass	expression,observed
R4632	T7045	T7044	amod	ectopic,expression
R4633	T7046	T7031	conj	observed,form
R4634	T7047	T7044	prep	of,expression
R4635	T7024	T7008	punct	", ",appears
R4636	T7048	T7047	pobj	Msx2,of
R4637	T7049	T7046	auxpass	is,observed
R4638	T7050	T7046	prep	in,observed
R4639	T7025	T7008	cc	and,appears
R464	T1354	T1355	mark	If,mediated
R4640	T7051	T7052	det	the,posterior
R4641	T7052	T7050	pobj	posterior,in
R4642	T7026	T7027	amod	more,five
R4643	T7053	T7054	punct	(,3O
R4644	T7054	T7046	parataxis	3O,observed
R4645	T7027	T7029	nummod	five,condensations
R4646	T7055	T7054	compound	Figure,3O
R4647	T7056	T7054	punct	),3O
R4648	T7057	T7031	punct	.,form
R4649	T7028	T7027	quantmod	than,five
R465	T1355	T1366	advcl	mediated,been
R4650	T7029	T7031	nsubj	condensations,form
R4651	T7030	T7029	compound	digit,condensations
R4652	T7031	T7008	conj	form,appears
R4653	T7032	T7031	prep	in,form
R4654	T7182	T7183	amod	Skeletal,Differentiation
R4655	T7184	T7183	prep	in,Differentiation
R4656	T7185	T7184	pobj	Limbs,in
R4657	T7186	T7185	acl	Developing,Limbs
R4658	T7187	T7186	prep	with,Developing
R4659	T7188	T7189	amod	Reduced,Levels
R466	T1356	T1355	advmod	indeed,mediated
R4660	T7189	T7187	pobj	Levels,with
R4661	T7190	T7189	prep	of,Levels
R4662	T7191	T7192	compound	BMP,Signaling
R4663	T7192	T7190	pobj	Signaling,of
R4664	T7194	T7195	nsubjpass	BMPs,discovered
R4665	T7196	T7195	auxpass	were,discovered
R4666	T7197	T7195	advmod	first,discovered
R4667	T7198	T7195	prep	in,discovered
R4668	T7199	T7200	det	the,context
R4669	T7200	T7198	pobj	context,in
R467	T1357	T1358	det	the,effects
R4670	T7201	T7200	prep	of,context
R4671	T7202	T7203	poss	their,activity
R4672	T7203	T7201	pobj	activity,of
R4673	T7204	T7203	amod	skeletogenic,activity
R4674	T7205	T7195	punct	.,discovered
R4675	T7207	T7208	nsubj	We,wanted
R4676	T7209	T7208	advmod	therefore,wanted
R4677	T7210	T7211	aux	to,determine
R4678	T7211	T7208	xcomp	determine,wanted
R4679	T7212	T7213	mark	whether,perturb
R468	T1358	T1355	nsubjpass	effects,mediated
R4680	T7213	T7211	ccomp	perturb,determine
R4681	T7214	T7215	det	the,loss
R4682	T7215	T7213	nsubj	loss,perturb
R4683	T7216	T7215	prep	of,loss
R4684	T7217	T7218	det	the,molecules
R4685	T7218	T7216	pobj	molecules,of
R4686	T7219	T7218	amod	specific,molecules
R4687	T7220	T7218	compound	BMP,molecules
R4688	T7221	T7218	prep	under,molecules
R4689	T7222	T7221	pobj	investigation,under
R469	T1359	T1360	amod	long,range
R4690	T7223	T7222	advmod	here,investigation
R4691	T7224	T7213	aux	would,perturb
R4692	T7225	T7226	det	the,pathways
R4693	T7226	T7213	dobj	pathways,perturb
R4694	T7227	T7228	amod	skeletal,differentiation
R4695	T7228	T7226	compound	differentiation,pathways
R4696	T7229	T7208	punct	.,wanted
R4697	T7231	T7232	advmod	Accordingly,subjected
R4698	T7233	T7232	punct	", ",subjected
R4699	T7234	T7235	amod	skeletal,preparations
R47	T206	T205	advmod	well,as
R470	T1360	T1358	compound	range,effects
R4700	T7235	T7232	nsubjpass	preparations,subjected
R4701	T7236	T7235	prep	from,preparations
R4702	T7237	T7236	pobj	each,from
R4703	T7238	T7237	prep	of,each
R4704	T7239	T7240	det	the,alleles
R4705	T7240	T7238	pobj	alleles,of
R4706	T7241	T7240	amod	various,alleles
R4707	T7242	T7240	compound	mutant,alleles
R4708	T7243	T7232	auxpass	were,subjected
R4709	T7244	T7232	prep	to,subjected
R471	T1361	T1360	punct	-,range
R4710	T7245	T7246	amod	histological,analyses
R4711	T7246	T7244	pobj	analyses,to
R4712	T7247	T7245	cc	and,histological
R4713	T7248	T7245	conj	molecular,histological
R4714	T7249	T7232	punct	.,subjected
R4715	T7251	T7252	det	The,elements
R4716	T7252	T7254	nsubj	elements,underwent
R4717	T7253	T7252	amod	skeletal,elements
R4718	T7255	T7256	dep	that,formed
R4719	T7256	T7252	relcl	formed,elements
R472	T1362	T1358	prep	of,effects
R4720	T7257	T7256	prep	in,formed
R4721	T7258	T7259	det	the,absence
R4722	T7259	T7257	pobj	absence,in
R4723	T7260	T7259	prep	of,absence
R4724	T7261	T7260	pobj	BMP2,of
R4725	T7262	T7261	punct	", ",BMP2
R4726	T7263	T7261	conj	BMP4,BMP2
R4727	T7264	T7263	punct	", ",BMP4
R4728	T7265	T7263	cc	or,BMP4
R4729	T7266	T7263	conj	BMP7,BMP4
R473	T1363	T1362	pobj	SHH,of
R4730	T7267	T7261	cc	or,BMP2
R4731	T7268	T7261	conj	BMP2,BMP2
R4732	T7269	T7268	cc	and,BMP2
R4733	T7270	T7268	conj	BMP7,BMP2
R4734	T7271	T7252	appos	all,elements
R4735	T7272	T7273	amod	normal,chondrogenesis
R4736	T7273	T7254	dobj	chondrogenesis,underwent
R4737	T7274	T7273	cc	and,chondrogenesis
R4738	T7275	T7273	conj	osteogenesis,chondrogenesis
R4739	T7276	T7254	prep	by,underwent
R474	T1364	T1355	auxpass	are,mediated
R4740	T7277	T7278	det	a,series
R4741	T7278	T7276	pobj	series,by
R4742	T7279	T7278	prep	of,series
R4743	T7280	T7279	pobj	criteria,of
R4744	T7281	T7254	punct	.,underwent
R4745	T7283	T7284	nsubj	All,showed
R4746	T7285	T7286	amod	normal,staining
R4747	T7286	T7284	dobj	staining,showed
R4748	T7287	T7284	prep	with,showed
R4749	T7288	T7289	amod	Alcian,blue
R475	T1365	T1355	advmod	indirectly,mediated
R4750	T7289	T7287	pobj	blue,with
R4751	T7290	T7291	punct	(,for
R4752	T7291	T7289	parataxis	for,blue
R4753	T7292	T7291	pobj	cartilage,for
R4754	T7293	T7291	punct	),for
R4755	T7294	T7289	cc	and,blue
R4756	T7295	T7296	amod	Alizarin,red
R4757	T7296	T7289	conj	red,blue
R4758	T7297	T7298	punct	(,see
R4759	T7298	T7296	parataxis	see,red
R476	T1367	T1355	prep	by,mediated
R4760	T7299	T7298	prep	for,see
R4761	T7300	T7301	amod	mineralized,tissue
R4762	T7301	T7299	pobj	tissue,for
R4763	T7302	T7298	punct	", ",see
R4764	T7303	T7298	dobj	Figure,see
R4765	T7304	T7303	nummod	1,Figure
R4766	T7305	T7298	punct	),see
R4767	T7306	T7284	punct	.,showed
R4768	T7308	T7309	nsubjpass	Most,analyzed
R4769	T7310	T7309	auxpass	were,analyzed
R477	T1368	T1369	amod	local,production
R4770	T7311	T7309	advmod	also,analyzed
R4771	T7312	T7309	prep	in,analyzed
R4772	T7313	T7314	amod	histological,sections
R4773	T7314	T7312	pobj	sections,in
R4774	T7315	T7314	acl	stained,sections
R4775	T7316	T7315	prep	with,stained
R4776	T7317	T7316	pobj	toluidine,with
R4777	T7318	T7317	amod	blue,toluidine
R4778	T7319	T7309	cc	and,analyzed
R4779	T7320	T7309	conj	showed,analyzed
R478	T1369	T1367	pobj	production,by
R4780	T7321	T7322	amod	osteoid,formation
R4781	T7322	T7320	dobj	formation,showed
R4782	T7323	T7322	cc	and,formation
R4783	T7324	T7325	compound	bone,matrix
R4784	T7325	T7326	compound	matrix,deposition
R4785	T7326	T7322	conj	deposition,formation
R4786	T7327	T7328	punct	(,compare
R4787	T7328	T7320	parataxis	compare,showed
R4788	T7329	T7330	compound	Figure,S2A
R4789	T7330	T7328	dobj	S2A,compare
R479	T1370	T1369	prep	of,production
R4790	T7331	T7330	cc	and,S2A
R4791	T7332	T7330	conj	S2E,S2A
R4792	T7333	T7328	punct	;,compare
R4793	T7334	T7328	cc	and,compare
R4794	T7335	T7336	amod	unpublished,data
R4795	T7336	T7328	conj	data,compare
R4796	T7337	T7328	punct	),compare
R4797	T7338	T7309	punct	.,analyzed
R4798	T7340	T7341	det	The,elements
R4799	T7341	T7343	nsubj	elements,contained
R48	T207	T200	conj	in,in
R480	T1371	T1372	amod	secondary,signals
R4800	T7342	T7341	amod	skeletal,elements
R4801	T7344	T7341	prep	in,elements
R4802	T7345	T7346	det	these,animals
R4803	T7346	T7344	pobj	animals,in
R4804	T7347	T7346	amod	various,animals
R4805	T7348	T7349	npadvmod	BMP,deficient
R4806	T7349	T7346	amod	deficient,animals
R4807	T7350	T7349	punct	-,deficient
R4808	T7351	T7352	amod	normal,chondrocytes
R4809	T7352	T7343	dobj	chondrocytes,contained
R481	T1372	T1370	pobj	signals,of
R4810	T7353	T7352	amod	prehypertrophic,chondrocytes
R4811	T7354	T7352	acl	expressing,chondrocytes
R4812	T7355	T7356	nmod	type,collagen
R4813	T7356	T7354	dobj	collagen,expressing
R4814	T7357	T7355	nummod	II,type
R4815	T7358	T7352	punct	", ",chondrocytes
R4816	T7359	T7360	amod	hypertrophic,chondrocytes
R4817	T7360	T7352	conj	chondrocytes,chondrocytes
R4818	T7361	T7360	acl	expressing,chondrocytes
R4819	T7362	T7363	nmod	type,collagen
R482	T1373	T1366	punct	", ",been
R4820	T7363	T7361	dobj	collagen,expressing
R4821	T7364	T7362	nummod	X,type
R4822	T7365	T7360	punct	", ",chondrocytes
R4823	T7366	T7367	advmod	as,as
R4824	T7367	T7360	cc	as,chondrocytes
R4825	T7368	T7367	advmod	well,as
R4826	T7369	T7370	det	the,osteoblasts
R4827	T7370	T7360	conj	osteoblasts,chondrocytes
R4828	T7371	T7370	acl	expressing,osteoblasts
R4829	T7372	T7373	nmod	type,collagen
R483	T1374	T1375	det	the,candidates
R4830	T7373	T7371	dobj	collagen,expressing
R4831	T7374	T7372	nummod	I,type
R4832	T7375	T7371	punct	", ",expressing
R4833	T7376	T7371	prep	like,expressing
R4834	T7377	T7378	poss	their,counterparts
R4835	T7378	T7376	pobj	counterparts,like
R4836	T7379	T7380	amod	wild,type
R4837	T7380	T7378	compound	type,counterparts
R4838	T7381	T7380	punct	-,type
R4839	T7382	T7383	punct	(,S2
R484	T1375	T1366	nsubj	candidates,been
R4840	T7383	T7343	parataxis	S2,contained
R4841	T7384	T7383	compound	Figure,S2
R4842	T7385	T7383	punct	;,S2
R4843	T7386	T7383	conj	compare,S2
R4844	T7387	T7388	compound	Figure,S2F
R4845	T7388	T7386	dobj	S2F,compare
R4846	T7389	T7390	punct	–,S2H
R4847	T7390	T7388	prep	S2H,S2F
R4848	T7391	T7386	prep	with,compare
R4849	T7392	T7393	compound	Figure,S2B
R485	T1376	T1375	amod	leading,candidates
R4850	T7393	T7391	pobj	S2B,with
R4851	T7394	T7395	punct	–,S2D
R4852	T7395	T7393	prep	S2D,S2B
R4853	T7396	T7386	punct	;,compare
R4854	T7397	T7386	cc	and,compare
R4855	T7398	T7399	amod	unpublished,data
R4856	T7399	T7386	conj	data,compare
R4857	T7400	T7383	punct	),S2
R4858	T7401	T7343	punct	.,contained
R4859	T7403	T7404	prep	In,showed
R486	T1377	T1366	aux	have,been
R4860	T7405	T7403	pobj	contrast,In
R4861	T7406	T7404	punct	", ",showed
R4862	T7407	T7404	advmod	however,showed
R4863	T7408	T7404	punct	", ",showed
R4864	T7409	T7404	nsubj	limbs,showed
R4865	T7410	T7411	advmod	completely,deficient
R4866	T7411	T7409	amod	deficient,limbs
R4867	T7412	T7411	prep	in,deficient
R4868	T7413	T7414	preconj	both,BMP2
R4869	T7414	T7415	nmod	BMP2,activity
R487	T1378	T1379	nummod	two,members
R4870	T7415	T7412	pobj	activity,in
R4871	T7416	T7414	cc	and,BMP2
R4872	T7417	T7414	conj	BMP4,BMP2
R4873	T7418	T7419	amod	severe,defects
R4874	T7419	T7404	dobj	defects,showed
R4875	T7420	T7419	prep	in,defects
R4876	T7421	T7422	amod	skeletal,differentiation
R4877	T7422	T7420	pobj	differentiation,in
R4878	T7423	T7404	punct	.,showed
R4879	T7592	T7593	nsubj	BMP2,Are
R488	T1379	T1366	attr	members,been
R4880	T7594	T7592	cc	and,BMP2
R4881	T7595	T7592	conj	BMP4,BMP2
R4882	T7596	T7593	neg	Not,Are
R4883	T7597	T7593	acomp	Critical,Are
R4884	T7598	T7597	prep	for,Critical
R4885	T7599	T7600	amod	Chondrogenic,Differentiation
R4886	T7600	T7598	pobj	Differentiation,for
R4887	T7602	T7603	mark	As,noted
R4888	T7603	T7604	advcl	noted,visualized
R4889	T7605	T7603	advmod	above,noted
R489	T1380	T1379	prep	of,members
R4890	T7606	T7604	punct	", ",visualized
R4891	T7607	T7604	prep	at,visualized
R4892	T7608	T7607	pobj	E12.5,at
R4893	T7609	T7604	punct	", ",visualized
R4894	T7610	T7611	amod	chondrogenic,condensation
R4895	T7611	T7604	nsubjpass	condensation,visualized
R4896	T7612	T7611	prep	of,condensation
R4897	T7613	T7614	det	the,digits
R4898	T7614	T7612	pobj	digits,of
R4899	T7615	T7604	aux	could,visualized
R49	T208	T209	det	the,process
R490	T1381	T1382	det	the,family
R4900	T7616	T7604	auxpass	be,visualized
R4901	T7617	T7604	prep	in,visualized
R4902	T7618	T7619	nmod	Bmp2C,C
R4903	T7619	T7621	nmod	C,buds
R4904	T7620	T7619	punct	/,C
R4905	T7621	T7617	pobj	buds,in
R4906	T7622	T7619	punct	;,C
R4907	T7623	T7624	compound	Bmp4C,C
R4908	T7624	T7619	appos	C,C
R4909	T7625	T7624	punct	/,C
R491	T1382	T1380	pobj	family,of
R4910	T7626	T7619	punct	;,C
R4911	T7627	T7628	compound	Prx1,cre
R4912	T7628	T7619	appos	cre,C
R4913	T7629	T7628	punct	:,cre
R4914	T7630	T7628	punct	:,cre
R4915	T7631	T7621	compound	limb,buds
R4916	T7632	T7604	prep	by,visualized
R4917	T7633	T7634	advmod	in,situ
R4918	T7634	T7635	amod	situ,hybridization
R4919	T7635	T7632	pobj	hybridization,by
R492	T1383	T1382	compound	BMP,family
R4920	T7636	T7635	prep	with,hybridization
R4921	T7637	T7638	det	a,probe
R4922	T7638	T7636	pobj	probe,with
R4923	T7639	T7638	compound	Sox9,probe
R4924	T7640	T7604	punct	.,visualized
R4925	T7642	T7643	amod	Similar,analysis
R4926	T7643	T7644	nsubj	analysis,demonstrated
R4927	T7645	T7644	prep	at,demonstrated
R4928	T7646	T7645	pobj	E10.5,at
R4929	T7647	T7646	cc	and,E10.5
R493	T1384	T1379	punct	", ",members
R4930	T7648	T7646	conj	E11.5,E10.5
R4931	T7649	T7650	det	a,process
R4932	T7650	T7644	dobj	process,demonstrated
R4933	T7651	T7652	advmod	relatively,normal
R4934	T7652	T7650	amod	normal,process
R4935	T7653	T7650	prep	of,process
R4936	T7654	T7653	pobj	condensation,of
R4937	T7655	T7654	prep	of,condensation
R4938	T7656	T7657	det	the,elements
R4939	T7657	T7655	pobj	elements,of
R494	T1385	T1379	appos	BMP2,members
R4940	T7658	T7657	amod	proximal,elements
R4941	T7659	T7657	amod	skeletal,elements
R4942	T7660	T7661	punct	(,S1H
R4943	T7661	T7650	parataxis	S1H,process
R4944	T7662	T7661	compound	Figure,S1H
R4945	T7663	T7664	punct	–,S1M
R4946	T7664	T7661	prep	S1M,S1H
R4947	T7665	T7661	punct	),S1H
R4948	T7666	T7644	punct	", ",demonstrated
R4949	T7667	T7644	prep	despite,demonstrated
R495	T1386	T1385	cc	and,BMP2
R4950	T7668	T7669	det	the,absence
R4951	T7669	T7667	pobj	absence,despite
R4952	T7670	T7669	prep	of,absence
R4953	T7671	T7672	amod	detectable,transcripts
R4954	T7672	T7670	pobj	transcripts,of
R4955	T7673	T7672	nmod	Bmp2,transcripts
R4956	T7674	T7673	cc	and,Bmp2
R4957	T7675	T7673	conj	Bmp4,Bmp2
R4958	T7676	T7644	punct	.,demonstrated
R4959	T7678	T7679	mark	As,shown
R496	T1387	T1385	conj	BMP7,BMP2
R4960	T7679	T7680	advcl	shown,observed
R4961	T7681	T7679	prep	in,shown
R4962	T7682	T7683	compound	Figure,S1N
R4963	T7683	T7681	pobj	S1N,in
R4964	T7684	T7683	cc	and,S1N
R4965	T7685	T7683	conj	S1O,S1N
R4966	T7686	T7680	punct	", ",observed
R4967	T7687	T7680	nsubjpass	expression,observed
R4968	T7688	T7687	prep	of,expression
R4969	T7689	T7688	pobj	Col,of
R497	T1388	T1366	punct	.,been
R4970	T7690	T7689	nummod	II,Col
R4971	T7691	T7689	punct	", ",Col
R4972	T7692	T7693	det	a,marker
R4973	T7693	T7689	appos	marker,Col
R4974	T7694	T7693	compound	chondrocyte,marker
R4975	T7695	T7680	punct	", ",observed
R4976	T7696	T7680	auxpass	is,observed
R4977	T7697	T7680	prep	in,observed
R4978	T7698	T7699	det	the,cartilage
R4979	T7699	T7697	pobj	cartilage,in
R498	T1390	T1391	nsubjpass	Both,expressed
R4980	T7700	T7701	nmod	BMP2,BMP4
R4981	T7701	T7703	npadvmod	BMP4,deficient
R4982	T7702	T7701	punct	", ",BMP4
R4983	T7703	T7699	amod	deficient,cartilage
R4984	T7704	T7703	punct	–,deficient
R4985	T7705	T7706	punct	(,S1O
R4986	T7706	T7699	parataxis	S1O,cartilage
R4987	T7707	T7706	compound	Figure,S1O
R4988	T7708	T7706	punct	),S1O
R4989	T7709	T7710	advmod	as,early
R499	T1392	T1391	auxpass	are,expressed
R4990	T7710	T7680	advmod	early,observed
R4991	T7711	T7710	prep	as,early
R4992	T7712	T7711	pobj	E12.5,as
R4993	T7713	T7680	punct	", ",observed
R4994	T7714	T7680	advcl	similar,observed
R4995	T7715	T7714	prep	to,similar
R4996	T7716	T7717	det	a,control
R4997	T7717	T7715	pobj	control,to
R4998	T7718	T7719	amod	wild,type
R4999	T7719	T7717	compound	type,control
R5	T159	T158	pobj	BMP2,of
R50	T209	T207	pobj	process,in
R500	T1393	T1391	prep	in,expressed
R5000	T7720	T7719	punct	-,type
R5001	T7721	T7722	punct	(,S1N
R5002	T7722	T7680	parataxis	S1N,observed
R5003	T7723	T7722	compound	Figure,S1N
R5004	T7724	T7722	punct	),S1N
R5005	T7725	T7680	punct	.,observed
R5006	T7727	T7728	advmod	Similarly,visualized
R5007	T7729	T7728	punct	", ",visualized
R5008	T7730	T7728	prep	at,visualized
R5009	T7731	T7730	pobj	E13.5,at
R501	T1394	T1395	det	a,domain
R5010	T7732	T7728	punct	", ",visualized
R5011	T7733	T7734	det	the,skeleton
R5012	T7734	T7728	nsubjpass	skeleton,visualized
R5013	T7735	T7734	amod	entire,skeleton
R5014	T7736	T7734	compound	limb,skeleton
R5015	T7737	T7728	aux	could,visualized
R5016	T7738	T7728	auxpass	be,visualized
R5017	T7739	T7728	xcomp	undergoing,visualized
R5018	T7740	T7739	dobj	chondrogenesis,undergoing
R5019	T7741	T7739	prep	by,undergoing
R502	T1395	T1393	pobj	domain,in
R5020	T7742	T7743	amod	Alcian,staining
R5021	T7743	T7741	pobj	staining,by
R5022	T7744	T7743	amod	blue,staining
R5023	T7745	T7739	punct	", ",undergoing
R5024	T7746	T7747	dep	albeit,in
R5025	T7747	T7739	prep	in,undergoing
R5026	T7748	T7749	det	a,pattern
R5027	T7749	T7747	pobj	pattern,in
R5028	T7750	T7749	amod	defective,pattern
R5029	T7751	T7739	punct	", ",undergoing
R503	T1396	T1395	amod	broader,domain
R5030	T7752	T7739	prep	in,undergoing
R5031	T7753	T7754	det	the,buds
R5032	T7754	T7752	pobj	buds,in
R5033	T7755	T7756	nmod	BMP2,BMP4
R5034	T7756	T7758	npadvmod	BMP4,deficient
R5035	T7757	T7756	punct	", ",BMP4
R5036	T7758	T7754	amod	deficient,buds
R5037	T7759	T7758	punct	–,deficient
R5038	T7760	T7754	compound	limb,buds
R5039	T7761	T7762	punct	(,4A
R504	T1397	T1395	prep	than,domain
R5040	T7762	T7728	parataxis	4A,visualized
R5041	T7763	T7762	compound	Figure,4A
R5042	T7764	T7762	cc	and,4A
R5043	T7765	T7762	conj	4B,4A
R5044	T7766	T7762	cc	and,4A
R5045	T7767	T7768	amod	unpublished,data
R5046	T7768	T7762	conj	data,4A
R5047	T7769	T7762	punct	),4A
R5048	T7770	T7728	punct	.,visualized
R5049	T7772	T7773	mark	As,grow
R505	T1398	T1397	pobj	SHH,than
R5050	T7773	T7777	advcl	grow,occurs
R5051	T7774	T7775	det	the,elements
R5052	T7775	T7773	nsubj	elements,grow
R5053	T7776	T7775	compound	cartilage,elements
R5054	T7778	T7777	punct	", ",occurs
R5055	T7779	T7780	det	a,wave
R5056	T7780	T7777	nsubj	wave,occurs
R5057	T7781	T7780	prep	of,wave
R5058	T7782	T7783	amod	hypertrophic,differentiation
R5059	T7783	T7781	pobj	differentiation,of
R506	T1399	T1391	prep	in,expressed
R5060	T7784	T7777	prep	from,occurs
R5061	T7785	T7786	det	the,center
R5062	T7786	T7784	pobj	center,from
R5063	T7787	T7784	prep	toward,from
R5064	T7788	T7789	det	the,ends
R5065	T7789	T7787	pobj	ends,toward
R5066	T7790	T7789	amod	distal,ends
R5067	T7791	T7792	punct	(,reviewed
R5068	T7792	T7777	parataxis	reviewed,occurs
R5069	T7793	T7792	prep	in,reviewed
R507	T1400	T1401	det	the,mesenchyme
R5070	T7794	T7793	punct	[,in
R5071	T7795	T7793	pobj	39,in
R5072	T7796	T7797	punct	–,41
R5073	T7797	T7795	prep	41,39
R5074	T7798	T7792	punct	],reviewed
R5075	T7799	T7792	punct	),reviewed
R5076	T7800	T7777	punct	.,occurs
R5077	T7802	T7803	amod	Histological,examination
R5078	T7803	T7804	nsubj	examination,showed
R5079	T7805	T7803	prep	of,examination
R508	T1401	T1399	pobj	mesenchyme,in
R5080	T7806	T7807	det	the,humeri
R5081	T7807	T7805	pobj	humeri,of
R5082	T7808	T7804	prep	at,showed
R5083	T7809	T7808	pobj	E13.5,at
R5084	T7810	T7811	mark	that,delayed
R5085	T7811	T7804	ccomp	delayed,showed
R5086	T7812	T7813	mark	while,taken
R5087	T7813	T7811	advcl	taken,delayed
R5088	T7814	T7815	amod	chondrogenic,differentiation
R5089	T7815	T7813	nsubj	differentiation,taken
R509	T1402	T1403	amod	early,bud
R5090	T7816	T7813	aux	had,taken
R5091	T7817	T7813	dobj	place,taken
R5092	T7818	T7813	prep	in,taken
R5093	T7819	T7820	det	the,humerus
R5094	T7820	T7818	pobj	humerus,in
R5095	T7821	T7822	nmod	BMP2,BMP4
R5096	T7822	T7824	npadvmod	BMP4,deficient
R5097	T7823	T7822	punct	", ",BMP4
R5098	T7824	T7820	amod	deficient,humerus
R5099	T7825	T7824	punct	–,deficient
R51	T210	T209	prep	of,process
R510	T1403	T1401	compound	bud,mesenchyme
R5100	T7826	T7811	punct	", ",delayed
R5101	T7827	T7828	amod	hypertrophic,differentiation
R5102	T7828	T7811	nsubjpass	differentiation,delayed
R5103	T7829	T7811	auxpass	was,delayed
R5104	T7830	T7831	punct	(,4C
R5105	T7831	T7804	parataxis	4C,showed
R5106	T7832	T7831	compound	Figure,4C
R5107	T7833	T7831	cc	and,4C
R5108	T7834	T7831	conj	4D,4C
R5109	T7835	T7831	punct	),4C
R511	T1404	T1403	amod	posterior,bud
R5110	T7836	T7804	punct	.,showed
R5111	T7838	T7839	advmod	However,was
R5112	T7840	T7839	punct	", ",was
R5113	T7841	T7839	prep	by,was
R5114	T7842	T7841	pobj	E15.5,by
R5115	T7843	T7839	punct	", ",was
R5116	T7844	T7839	nsubj	hypertrophy,was
R5117	T7845	T7839	acomp	evident,was
R5118	T7846	T7839	prep	in,was
R5119	T7847	T7848	det	these,elements
R512	T1405	T1403	compound	limb,bud
R5120	T7848	T7846	pobj	elements,in
R5121	T7849	T7850	mark	as,seen
R5122	T7850	T7839	advcl	seen,was
R5123	T7851	T7850	prep	by,seen
R5124	T7852	T7853	nmod	type,expression
R5125	T7853	T7851	pobj	expression,by
R5126	T7854	T7852	punct	X,type
R5127	T7855	T7853	compound	collagen,expression
R5128	T7856	T7857	punct	(,4E
R5129	T7857	T7839	parataxis	4E,was
R513	T1406	T1407	punct	[,7
R5130	T7858	T7857	compound	Figure,4E
R5131	T7859	T7857	cc	and,4E
R5132	T7860	T7857	conj	4F,4E
R5133	T7861	T7857	punct	),4E
R5134	T7862	T7839	punct	.,was
R5135	T7864	T7865	det	The,cells
R5136	T7865	T7867	nsubj	cells,express
R5137	T7866	T7865	amod	hypertrophic,cells
R5138	T7868	T7865	prep	in,cells
R5139	T7869	T7870	nmod	BMP2,BMP4
R514	T1407	T1391	parataxis	7,expressed
R5140	T7870	T7872	npadvmod	BMP4,deficient
R5141	T7871	T7870	punct	", ",BMP4
R5142	T7872	T7874	amod	deficient,limb
R5143	T7873	T7872	punct	–,deficient
R5144	T7874	T7868	pobj	limb,in
R5145	T7875	T7867	advmod	also,express
R5146	T7876	T7877	amod	late,markers
R5147	T7877	T7867	dobj	markers,express
R5148	T7878	T7877	amod	hypertrophic,markers
R5149	T7879	T7877	punct	", ",markers
R515	T1408	T1407	nummod	6,7
R5150	T7880	T7881	amod	such,as
R5151	T7881	T7877	prep	as,markers
R5152	T7882	T7881	pobj	osteopontin,as
R5153	T7883	T7884	punct	[,42
R5154	T7884	T7882	parataxis	42,osteopontin
R5155	T7885	T7884	punct	],42
R5156	T7886	T7887	punct	(,data
R5157	T7887	T7867	meta	data,express
R5158	T7888	T7887	amod	unpublished,data
R5159	T7889	T7887	punct	),data
R516	T1409	T1407	punct	",",7
R5160	T7890	T7867	punct	.,express
R5161	T7892	T7893	aux	To,examine
R5162	T7893	T7894	advcl	examine,carried
R5163	T7895	T7896	mark	whether,is
R5164	T7896	T7893	ccomp	is,examine
R5165	T7897	T7898	det	the,execution
R5166	T7898	T7896	nsubj	execution,is
R5167	T7899	T7898	amod	successful,execution
R5168	T7900	T7898	prep	of,execution
R5169	T7901	T7900	pobj	chondrogenesis,of
R517	T1410	T1407	punct	],7
R5170	T7902	T7898	prep	in,execution
R5171	T7903	T7904	det	the,limbs
R5172	T7904	T7902	pobj	limbs,in
R5173	T7905	T7906	nmod	BMP2,BMP4
R5174	T7906	T7908	npadvmod	BMP4,deficient
R5175	T7907	T7906	punct	", ",BMP4
R5176	T7908	T7904	amod	deficient,limbs
R5177	T7909	T7908	punct	–,deficient
R5178	T7910	T7911	det	a,result
R5179	T7911	T7896	attr	result,is
R518	T1411	T1391	punct	", ",expressed
R5180	T7912	T7911	prep	of,result
R5181	T7913	T7914	amod	compensatory,upregulation
R5182	T7914	T7912	pobj	upregulation,of
R5183	T7915	T7914	prep	of,upregulation
R5184	T7916	T7917	amod	other,BMPs
R5185	T7917	T7915	pobj	BMPs,of
R5186	T7918	T7917	amod	ostegenic,BMPs
R5187	T7919	T7894	punct	", ",carried
R5188	T7920	T7894	nsubj	we,carried
R5189	T7921	T7894	prt	out,carried
R519	T1412	T1413	mark	although,has
R5190	T7922	T7923	advmod	in,situ
R5191	T7923	T7924	amod	situ,hybridization
R5192	T7924	T7894	dobj	hybridization,carried
R5193	T7925	T7894	prep	for,carried
R5194	T7926	T7925	pobj	Bmp6,for
R5195	T7927	T7926	cc	and,Bmp6
R5196	T7928	T7926	conj	Bmp7,Bmp6
R5197	T7929	T7894	prep	in,carried
R5198	T7930	T7931	det	these,limbs
R5199	T7931	T7929	pobj	limbs,in
R52	T211	T210	pobj	skeletogenesis,of
R520	T1413	T1391	advcl	has,expressed
R5200	T7932	T7894	punct	.,carried
R5201	T7934	T7935	prep	At,observed
R5202	T7936	T7934	pobj	E13.5,At
R5203	T7937	T7935	punct	", ",observed
R5204	T7938	T7935	nsubj	we,observed
R5205	T7939	T7940	det	no,increase
R5206	T7940	T7935	dobj	increase,observed
R5207	T7941	T7940	prep	in,increase
R5208	T7942	T7941	pobj	expression,in
R5209	T7943	T7942	prep	of,expression
R521	T1414	T1413	nsubj	BMP7,has
R5210	T7944	T7945	nmod	Bmp6,mRNA
R5211	T7945	T7943	pobj	mRNA,of
R5212	T7946	T7947	punct	(,S1P
R5213	T7947	T7944	parataxis	S1P,Bmp6
R5214	T7948	T7947	compound	Figure,S1P
R5215	T7949	T7950	punct	–,S1S
R5216	T7950	T7947	prep	S1S,S1P
R5217	T7951	T7947	punct	),S1P
R5218	T7952	T7944	cc	or,Bmp6
R5219	T7953	T7944	conj	Bmp7,Bmp6
R522	T1415	T1413	advmod	also,has
R5220	T7954	T7955	punct	(,compare
R5221	T7955	T7953	parataxis	compare,Bmp7
R5222	T7956	T7957	compound	Figure,4H
R5223	T7957	T7955	dobj	4H,compare
R5224	T7958	T7955	prep	with,compare
R5225	T7959	T7960	compound	Figure,4G
R5226	T7960	T7958	pobj	4G,with
R5227	T7961	T7955	punct	),compare
R5228	T7962	T7942	prep	in,expression
R5229	T7963	T7964	det	the,tissues
R523	T1416	T1417	det	a,domain
R5230	T7964	T7962	pobj	tissues,in
R5231	T7965	T7964	compound	mutant,tissues
R5232	T7966	T7935	prep	compared,observed
R5233	T7967	T7966	prep	to,compared
R5234	T7968	T7969	poss	their,counterparts
R5235	T7969	T7967	pobj	counterparts,to
R5236	T7970	T7971	amod	wild,type
R5237	T7971	T7969	compound	type,counterparts
R5238	T7972	T7971	punct	-,type
R524	T1417	T1413	dobj	domain,has
R5243	T8396	T8397	nsubjpass	Osteogenesis,Initiated
R5244	T8398	T8397	auxpass	Is,Initiated
R5245	T8399	T8397	advmod	Normally,Initiated
R5246	T8400	T8397	prep	in,Initiated
R5247	T8401	T8402	nmod	BMP2,BMP4
R5248	T8402	T8404	npadvmod	BMP4,Deficient
R5249	T8403	T8402	punct	", ",BMP4
R525	T1418	T1417	amod	second,domain
R5250	T8404	T8406	amod	Deficient,Buds
R5251	T8405	T8404	punct	–,Deficient
R5252	T8406	T8400	pobj	Buds,in
R5253	T8407	T8406	compound	Limb,Buds
R5254	T8409	T8410	compound	Bone,formation
R5255	T8410	T8411	nsubj	formation,occurs
R5256	T8412	T8411	advmod	first,occurs
R5257	T8413	T8411	prep	at,occurs
R5258	T8414	T8415	det	the,interface
R5259	T8415	T8413	pobj	interface,at
R526	T1419	T1417	punct	", ",domain
R5260	T8416	T8415	prep	between,interface
R5261	T8417	T8418	det	the,chondrocytes
R5262	T8418	T8416	pobj	chondrocytes,between
R5263	T8419	T8418	amod	late,chondrocytes
R5264	T8420	T8418	amod	hypertrophic,chondrocytes
R5265	T8421	T8418	cc	and,chondrocytes
R5266	T8422	T8423	det	the,perichondrium
R5267	T8423	T8418	conj	perichondrium,chondrocytes
R5268	T8424	T8423	amod	surrounding,perichondrium
R5269	T8425	T8415	punct	", ",interface
R527	T1420	T1417	amod	weaker,domain
R5270	T8426	T8427	det	a,region
R5271	T8427	T8415	appos	region,interface
R5272	T8428	T8427	acl	identified,region
R5273	T8429	T8428	prep	as,identified
R5274	T8430	T8431	det	the,collar
R5275	T8431	T8429	pobj	collar,as
R5276	T8432	T8431	compound	bone,collar
R5277	T8433	T8411	punct	.,occurs
R5278	T8435	T8436	nsubj	We,examined
R5279	T8437	T8438	mark	whether,initiated
R528	T1421	T1417	prep	of,domain
R5280	T8438	T8436	ccomp	initiated,examined
R5281	T8439	T8440	det	the,program
R5282	T8440	T8438	nsubjpass	program,initiated
R5283	T8441	T8440	compound	osteogenesis,program
R5284	T8442	T8438	auxpass	is,initiated
R5285	T8443	T8438	prep	in,initiated
R5286	T8444	T8445	det	the,limbs
R5287	T8445	T8443	pobj	limbs,in
R5288	T8446	T8447	nmod	BMP2,BMP4
R5289	T8447	T8449	npadvmod	BMP4,deficient
R529	T1422	T1421	pobj	expression,of
R5290	T8448	T8447	punct	", ",BMP4
R5291	T8449	T8445	amod	deficient,limbs
R5292	T8450	T8449	punct	–,deficient
R5293	T8451	T8436	advcl	using,examined
R5294	T8452	T8453	amod	several,criteria
R5295	T8453	T8451	dobj	criteria,using
R5296	T8454	T8453	amod	histological,criteria
R5297	T8455	T8454	cc	and,histological
R5298	T8456	T8454	conj	molecular,histological
R5299	T8457	T8451	punct	", ",using
R53	T212	T197	punct	.,implicated
R530	T1423	T1413	prep	in,has
R5300	T8458	T8459	mark	as,described
R5301	T8459	T8451	advcl	described,using
R5302	T8460	T8459	advmod	below,described
R5303	T8461	T8462	punct	(,Figure
R5304	T8462	T8459	parataxis	Figure,described
R5305	T8463	T8462	nummod	5,Figure
R5306	T8464	T8462	punct	),Figure
R5307	T8465	T8436	punct	.,examined
R5308	T8467	T8468	advcl	Adjacent,laid
R5309	T8469	T8467	prep	to,Adjacent
R531	T1424	T1425	det	the,mesenchyme
R5310	T8470	T8471	det	the,cells
R5311	T8471	T8469	pobj	cells,to
R5312	T8472	T8471	amod	hypertrophic,cells
R5313	T8473	T8468	punct	", ",laid
R5314	T8474	T8475	det	a,collar
R5315	T8475	T8468	nsubjpass	collar,laid
R5316	T8476	T8475	compound	bone,collar
R5317	T8477	T8468	auxpass	is,laid
R5318	T8478	T8468	prt	down,laid
R5319	T8479	T8468	advmod	normally,laid
R532	T1425	T1423	pobj	mesenchyme,in
R5320	T8480	T8468	prep	in,laid
R5321	T8481	T8482	det	the,absence
R5322	T8482	T8480	pobj	absence,in
R5323	T8483	T8482	prep	of,absence
R5324	T8484	T8483	pobj	BMP2,of
R5325	T8485	T8484	cc	and,BMP2
R5326	T8486	T8484	conj	BMP4,BMP2
R5327	T8487	T8488	punct	(,star
R5328	T8488	T8468	parataxis	star,laid
R5329	T8489	T8490	compound	Figure,5A
R533	T1426	T1427	amod	anterior,bud
R5330	T8490	T8488	dep	5A,star
R5331	T8491	T8490	cc	and,5A
R5332	T8492	T8490	conj	5B,5A
R5333	T8493	T8488	punct	", ",star
R5334	T8494	T8488	amod	red,star
R5335	T8495	T8488	punct	),star
R5336	T8496	T8468	punct	.,laid
R5337	T8498	T8499	mark	As,seen
R5338	T8499	T8502	advcl	seen,express
R5339	T8500	T8499	aux	can,seen
R534	T1427	T1425	compound	bud,mesenchyme
R5340	T8501	T8499	auxpass	be,seen
R5341	T8503	T8499	prep	in,seen
R5342	T8504	T8505	amod	sagittal,sections
R5343	T8505	T8503	pobj	sections,in
R5344	T8506	T8499	prep	through,seen
R5345	T8507	T8508	det	an,humerus
R5346	T8508	T8506	pobj	humerus,through
R5347	T8509	T8508	compound	E15.5,humerus
R5348	T8510	T8502	punct	", ",express
R5349	T8511	T8512	det	the,cells
R535	T1428	T1427	compound	limb,bud
R5350	T8512	T8502	nsubj	cells,express
R5351	T8513	T8512	acl	embedded,cells
R5352	T8514	T8513	prep	in,embedded
R5353	T8515	T8516	det	the,matrix
R5354	T8516	T8514	pobj	matrix,in
R5355	T8517	T8516	amod	mineralized,matrix
R5356	T8518	T8519	det	the,marker
R5357	T8519	T8502	dobj	marker,express
R5358	T8520	T8519	compound	osteoblast,marker
R5359	T8521	T8519	appos	Col,marker
R536	T1429	T1391	punct	.,expressed
R5360	T8522	T8521	nummod	I,Col
R5361	T8523	T8502	prep	as,express
R5362	T8524	T8523	amod	normal,as
R5363	T8525	T8526	punct	(,5C
R5364	T8526	T8502	parataxis	5C,express
R5365	T8527	T8526	compound	Figure,5C
R5366	T8528	T8526	cc	and,5C
R5367	T8529	T8526	conj	5D,5C
R5368	T8530	T8526	punct	),5C
R5369	T8531	T8502	punct	.,express
R537	T1431	T1432	nsubjpass	BMP2,induced
R5370	T8533	T8534	prep	By,undergoes
R5371	T8535	T8533	pobj	E17.5,By
R5372	T8536	T8534	punct	", ",undergoes
R5373	T8537	T8538	det	the,cartilage
R5374	T8538	T8534	nsubj	cartilage,undergoes
R5375	T8539	T8538	amod	mineralized,cartilage
R5376	T8540	T8541	dep	that,makes
R5377	T8541	T8538	relcl	makes,cartilage
R5378	T8542	T8541	prt	up,makes
R5379	T8543	T8544	det	the,zone
R538	T1433	T1434	punct	[,8
R5380	T8544	T8541	dobj	zone,makes
R5381	T8545	T8544	amod	late,zone
R5382	T8546	T8544	amod	hypertrophic,zone
R5383	T8547	T8544	prep	of,zone
R5384	T8548	T8549	det	the,plate
R5385	T8549	T8547	pobj	plate,of
R5386	T8550	T8549	compound	growth,plate
R5387	T8551	T8534	advmod	normally,undergoes
R5388	T8552	T8534	dobj	removal,undergoes
R5389	T8553	T8552	prep	by,removal
R539	T1434	T1431	parataxis	8,BMP2
R5390	T8554	T8553	pobj	osteoclasts,by
R5391	T8555	T8556	punct	(,arrows
R5392	T8556	T8534	parataxis	arrows,undergoes
R5393	T8557	T8558	compound	Figure,5G
R5394	T8558	T8556	dep	5G,arrows
R5395	T8559	T8556	punct	", ",arrows
R5396	T8560	T8561	amod	brown,stained
R5397	T8561	T8563	amod	stained,cells
R5398	T8562	T8561	punct	-,stained
R5399	T8563	T8556	dep	cells,arrows
R54	T214	T215	advmod	However,been
R540	T1435	T1434	punct	],8
R5400	T8564	T8556	punct	", ",arrows
R5401	T8565	T8556	amod	red,arrows
R5402	T8566	T8556	punct	),arrows
R5403	T8567	T8534	punct	", ",undergoes
R5404	T8568	T8534	advcl	resulting,undergoes
R5405	T8569	T8568	prep	in,resulting
R5406	T8570	T8571	det	the,formation
R5407	T8571	T8569	pobj	formation,in
R5408	T8572	T8571	prep	of,formation
R5409	T8573	T8574	det	a,cavity
R541	T1436	T1431	cc	and,BMP2
R5410	T8574	T8572	pobj	cavity,of
R5411	T8575	T8576	compound	bone,marrow
R5412	T8576	T8574	compound	marrow,cavity
R5413	T8577	T8578	punct	(,arrow
R5414	T8578	T8534	parataxis	arrow,undergoes
R5415	T8579	T8578	amod	red,arrow
R5416	T8580	T8578	prep	in,arrow
R5417	T8581	T8582	compound	Figure,5E
R5418	T8582	T8580	pobj	5E,in
R5419	T8583	T8582	cc	and,5E
R542	T1437	T1431	conj	BMP7,BMP2
R5420	T8584	T8582	conj	5I,5E
R5421	T8585	T8578	punct	", ",arrow
R5422	T8586	T8578	advcl	see,arrow
R5423	T8587	T8586	advmod	also,see
R5424	T8588	T8586	dobj	Figure,see
R5425	T8589	T8588	nummod	6,Figure
R5426	T8590	T8578	punct	),arrow
R5427	T8591	T8534	punct	.,undergoes
R5428	T8593	T8594	compound	Osteoprogenitor,cells
R5429	T8594	T8595	nsubj	cells,populate
R543	T1438	T1439	punct	[,7
R5430	T8596	T8594	prep	from,cells
R5431	T8597	T8598	compound	bone,region
R5432	T8598	T8596	pobj	region,from
R5433	T8599	T8598	compound	collar,region
R5434	T8600	T8595	advmod	now,populate
R5435	T8601	T8602	det	this,site
R5436	T8602	T8595	dobj	site,populate
R5437	T8603	T8595	cc	and,populate
R5438	T8604	T8595	conj	deposit,populate
R5439	T8605	T8604	dobj	osteoid,deposit
R544	T1439	T1437	parataxis	7,BMP7
R5440	T8606	T8595	punct	", ",populate
R5441	T8607	T8595	advcl	forming,populate
R5442	T8608	T8609	det	the,bone
R5443	T8609	T8607	dobj	bone,forming
R5444	T8610	T8609	amod	trabecular,bone
R5445	T8611	T8609	prep	in,bone
R5446	T8612	T8613	det	the,cavity
R5447	T8613	T8611	pobj	cavity,in
R5448	T8614	T8613	compound	bone,cavity
R5449	T8615	T8613	compound	marrow,cavity
R545	T1440	T1439	punct	],7
R5450	T8616	T8617	punct	(,arrow
R5451	T8617	T8613	parataxis	arrow,cavity
R5452	T8618	T8619	compound	Figure,6E
R5453	T8619	T8617	dep	6E,arrow
R5454	T8620	T8617	punct	", ",arrow
R5455	T8621	T8617	amod	red,arrow
R5456	T8622	T8617	punct	),arrow
R5457	T8623	T8613	cc	and,cavity
R5458	T8624	T8625	amod	cortical,bone
R5459	T8625	T8613	conj	bone,cavity
R546	T1441	T1432	aux	can,induced
R5460	T8626	T8607	prep	at,forming
R5461	T8627	T8626	pobj	birth,at
R5462	T8628	T8629	punct	(,asterisk
R5463	T8629	T8595	parataxis	asterisk,populate
R5464	T8630	T8631	compound	Figure,5I
R5465	T8631	T8629	dep	5I,asterisk
R5466	T8632	T8629	punct	", ",asterisk
R5467	T8633	T8629	punct	),asterisk
R5468	T8634	T8595	punct	.,populate
R5469	T8636	T8637	prep	In,is
R547	T1442	T1432	auxpass	be,induced
R5470	T8638	T8636	pobj	contrast,In
R5471	T8639	T8637	punct	", ",is
R5472	T8640	T8637	prep	in,is
R5473	T8641	T8642	compound	E17.5,limbs
R5474	T8642	T8640	pobj	limbs,in
R5475	T8643	T8642	amod	deficient,limbs
R5476	T8644	T8643	prep	in,deficient
R5477	T8645	T8644	pobj	BMP2,in
R5478	T8646	T8645	cc	and,BMP2
R5479	T8647	T8645	conj	BMP4,BMP2
R548	T1443	T1432	prep	by,induced
R5480	T8648	T8637	punct	", ",is
R5481	T8649	T8637	expl	there,is
R5482	T8650	T8651	det	an,delay
R5483	T8651	T8637	attr	delay,is
R5484	T8652	T8651	amod	overall,delay
R5485	T8653	T8651	prep	in,delay
R5486	T8654	T8655	det	the,process
R5487	T8655	T8653	pobj	process,in
R5488	T8656	T8655	amod	normal,process
R5489	T8657	T8655	amod	endochondral,process
R549	T1444	T1445	amod	ectopic,SHH
R5490	T8658	T8659	punct	(,5F
R5491	T8659	T8637	parataxis	5F,is
R5492	T8660	T8659	compound	Figure,5F
R5493	T8661	T8659	punct	),5F
R5494	T8662	T8637	punct	.,is
R5495	T8664	T8665	mark	While,occurs
R5496	T8665	T8667	advcl	occurs,recruited
R5497	T8666	T8665	nsubj	vascularization,occurs
R5498	T8668	T8665	prep	in,occurs
R5499	T8669	T8670	nmod	Bmp2C,C
R55	T216	T215	punct	", ",been
R550	T1445	T1443	pobj	SHH,by
R5500	T8670	T8672	nmod	C,forelimbs
R5501	T8671	T8670	punct	/,C
R5502	T8672	T8668	pobj	forelimbs,in
R5503	T8673	T8670	punct	;,C
R5504	T8674	T8675	compound	Bmp4C,C
R5505	T8675	T8670	appos	C,C
R5506	T8676	T8675	punct	/,C
R5507	T8677	T8670	punct	;,C
R5508	T8678	T8679	compound	Prx1,cre
R5509	T8679	T8670	appos	cre,C
R551	T1446	T1432	cc	and,induced
R5510	T8680	T8679	punct	:,cre
R5511	T8681	T8679	punct	:,cre
R5512	T8682	T8667	punct	", ",recruited
R5513	T8683	T8684	amod	few,osteoclasts
R5514	T8684	T8667	nsubjpass	osteoclasts,recruited
R5515	T8685	T8667	auxpass	are,recruited
R5516	T8686	T8667	prep	to,recruited
R5517	T8687	T8688	det	the,cartilage
R5518	T8688	T8686	pobj	cartilage,to
R5519	T8689	T8688	amod	mineralized,cartilage
R552	T1447	T1448	poss	their,expression
R5520	T8690	T8691	punct	(,arrows
R5521	T8691	T8667	parataxis	arrows,recruited
R5522	T8692	T8691	advcl	compare,arrows
R5523	T8693	T8694	compound	Figure,5G
R5524	T8694	T8692	dobj	5G,compare
R5525	T8695	T8694	cc	and,5G
R5526	T8696	T8697	compound	Figure,5H
R5527	T8697	T8694	conj	5H,5G
R5528	T8698	T8691	punct	", ",arrows
R5529	T8699	T8691	amod	red,arrows
R553	T1448	T1449	nsubjpass	expression,diminished
R5530	T8700	T8691	punct	),arrows
R5531	T8701	T8667	punct	", ",recruited
R5532	T8702	T8667	advcl	resulting,recruited
R5533	T8703	T8702	prep	in,resulting
R5534	T8704	T8705	det	a,delay
R5535	T8705	T8703	pobj	delay,in
R5536	T8706	T8705	prep	in,delay
R5537	T8707	T8708	compound	bone,marrow
R5538	T8708	T8709	compound	marrow,formation
R5539	T8709	T8706	pobj	formation,in
R554	T1449	T1432	conj	diminished,induced
R5540	T8710	T8709	compound	cavity,formation
R5541	T8711	T8702	prep	in,resulting
R5542	T8712	T8713	det	the,absence
R5543	T8713	T8711	pobj	absence,in
R5544	T8714	T8713	prep	of,absence
R5545	T8715	T8714	pobj	BMP2,of
R5546	T8716	T8715	cc	and,BMP2
R5547	T8717	T8715	conj	BMP4,BMP2
R5548	T8718	T8667	punct	.,recruited
R5549	T8720	T8721	prep	In,started
R555	T1450	T1449	auxpass	is,diminished
R5550	T8722	T8720	pobj	fact,In
R5551	T8723	T8721	punct	", ",started
R5552	T8724	T8725	compound	bone,marrow
R5553	T8725	T8726	compound	marrow,formation
R5554	T8726	T8721	nsubj	formation,started
R5555	T8727	T8726	cc	and,formation
R5556	T8728	T8729	amod	trabecular,formation
R5557	T8729	T8726	conj	formation,formation
R5558	T8730	T8729	compound	bone,formation
R5559	T8731	T8721	aux	have,started
R556	T1451	T1449	advmod	greatly,diminished
R5560	T8732	T8721	neg	not,started
R5561	T8733	T8721	advmod	yet,started
R5562	T8734	T8721	prep	at,started
R5563	T8735	T8734	pobj	birth,at
R5564	T8736	T8721	prep	in,started
R5565	T8737	T8738	det	the,absence
R5566	T8738	T8736	pobj	absence,in
R5567	T8739	T8738	prep	of,absence
R5568	T8740	T8739	pobj	BMP2,of
R5569	T8741	T8740	cc	and,BMP2
R557	T1452	T1449	prep	in,diminished
R5570	T8742	T8740	conj	BMP4,BMP2
R5571	T8743	T8744	punct	(,5J
R5572	T8744	T8721	parataxis	5J,started
R5573	T8745	T8744	compound	Figure,5J
R5574	T8746	T8744	punct	),5J
R5575	T8747	T8721	punct	", ",started
R5576	T8748	T8721	cc	and,started
R5577	T8749	T8750	det	the,morphology
R5578	T8750	T8751	nsubj	morphology,is
R5579	T8751	T8721	conj	is,started
R558	T1453	T1454	det	the,absence
R5580	T8752	T8750	prep	of,morphology
R5581	T8753	T8754	nmod	Bmp2C,C
R5582	T8754	T8756	nmod	C,bones
R5583	T8755	T8754	punct	/,C
R5584	T8756	T8752	pobj	bones,of
R5585	T8757	T8754	punct	;,C
R5586	T8758	T8759	compound	Bmp4C,C
R5587	T8759	T8754	appos	C,C
R5588	T8760	T8759	punct	/,C
R5589	T8761	T8754	punct	;,C
R559	T1454	T1452	pobj	absence,in
R5590	T8762	T8763	compound	Prx1,cre
R5591	T8763	T8754	appos	cre,C
R5592	T8764	T8763	punct	:,cre
R5593	T8765	T8763	punct	:,cre
R5594	T8766	T8756	prep	at,bones
R5595	T8767	T8766	pobj	birth,at
R5596	T8768	T8769	advmod	most,similar
R5597	T8769	T8751	acomp	similar,is
R5598	T8770	T8769	prep	to,similar
R5599	T8771	T8772	compound	control,embryos
R56	T217	T215	prep	due,been
R560	T1455	T1454	prep	of,absence
R5600	T8772	T8770	pobj	embryos,to
R5601	T8773	T8772	prep	at,embryos
R5602	T8774	T8773	pobj	E17.5,at
R5603	T8775	T8776	punct	(,5E
R5604	T8776	T8721	parataxis	5E,started
R5605	T8777	T8776	compound	Figure,5E
R5606	T8778	T8776	punct	),5E
R5607	T8779	T8721	punct	.,started
R5608	T8781	T8782	aux	To,differentiate
R5609	T8782	T8783	advcl	differentiate,performed
R561	T1456	T1457	compound	SHH,activity
R5610	T8784	T8782	prep	between,differentiate
R5611	T8785	T8786	det	a,delay
R5612	T8786	T8784	pobj	delay,between
R5613	T8787	T8786	amod	simple,delay
R5614	T8788	T8786	prep	in,delay
R5615	T8789	T8790	compound	bone,formation
R5616	T8790	T8788	pobj	formation,in
R5617	T8791	T8786	prep	in,delay
R5618	T8792	T8793	det	the,absence
R5619	T8793	T8791	pobj	absence,in
R562	T1457	T1455	pobj	activity,of
R5620	T8794	T8793	prep	of,absence
R5621	T8795	T8794	pobj	BMP2,of
R5622	T8796	T8795	cc	and,BMP2
R5623	T8797	T8795	conj	BMP4,BMP2
R5624	T8798	T8786	cc	and,delay
R5625	T8799	T8800	det	the,inability
R5626	T8800	T8786	conj	inability,delay
R5627	T8801	T8802	aux	to,form
R5628	T8802	T8800	acl	form,inability
R5629	T8803	T8802	dobj	bone,form
R563	T1458	T1459	punct	[,9
R5630	T8804	T8803	prep	without,bone
R5631	T8805	T8806	det	these,BMPs
R5632	T8806	T8804	pobj	BMPs,without
R5633	T8807	T8783	punct	", ",performed
R5634	T8808	T8783	nsubj	we,performed
R5635	T8809	T8810	amod	histological,analyses
R5636	T8810	T8783	dobj	analyses,performed
R5637	T8811	T8783	prep	in,performed
R5638	T8812	T8813	nmod	Bmp2C,C
R5639	T8813	T8815	nmod	C,mice
R564	T1459	T1449	parataxis	9,diminished
R5640	T8814	T8813	punct	/,C
R5641	T8815	T8811	pobj	mice,in
R5642	T8816	T8813	punct	;,C
R5643	T8817	T8818	compound	Bmp4C,C
R5644	T8818	T8813	appos	C,C
R5645	T8819	T8818	punct	/,C
R5646	T8820	T8813	punct	;,C
R5647	T8821	T8822	compound	Prx1,cre
R5648	T8822	T8813	appos	cre,C
R5649	T8823	T8822	punct	:,cre
R565	T1460	T1459	punct	],9
R5650	T8824	T8822	punct	:,cre
R5651	T8825	T8826	advmod	up,3
R5652	T8826	T8828	nummod	3,wks
R5653	T8827	T8826	quantmod	to,3
R5654	T8828	T8815	npadvmod	wks,mice
R5655	T8829	T8828	prep	of,wks
R5656	T8830	T8829	pobj	age,of
R5657	T8831	T8783	punct	.,performed
R5658	T8833	T8834	mark	As,shown
R5659	T8834	T8835	advcl	shown,found
R566	T1461	T1449	punct	.,diminished
R5660	T8836	T8834	prep	in,shown
R5661	T8837	T8838	compound	Figure,6A
R5662	T8838	T8836	pobj	6A,in
R5663	T8839	T8838	prep	through,6A
R5664	T8840	T8839	pobj	6E,through
R5665	T8841	T8835	punct	", ",found
R5666	T8842	T8835	prep	in,found
R5667	T8843	T8844	det	the,mice
R5668	T8844	T8842	pobj	mice,in
R5669	T8845	T8844	compound	control,mice
R567	T1463	T1464	nsubj	BMP2,are
R5670	T8846	T8835	punct	", ",found
R5671	T8847	T8848	det	a,collar
R5672	T8848	T8835	nsubjpass	collar,found
R5673	T8849	T8848	compound	bone,collar
R5674	T8850	T8835	auxpass	is,found
R5675	T8851	T8835	oprd	adjacent,found
R5676	T8852	T8851	prep	to,adjacent
R5677	T8853	T8854	det	the,zone
R5678	T8854	T8852	pobj	zone,to
R5679	T8855	T8854	prep	of,zone
R568	T1465	T1463	cc	and,BMP2
R5680	T8856	T8857	amod	late,chondrocytes
R5681	T8857	T8855	pobj	chondrocytes,of
R5682	T8858	T8857	amod	hypertrophic,chondrocytes
R5683	T8859	T8835	prep	at,found
R5684	T8860	T8861	nummod	1,wk
R5685	T8861	T8859	pobj	wk,at
R5686	T8862	T8863	punct	(,6A
R5687	T8863	T8835	parataxis	6A,found
R5688	T8864	T8863	compound	Figure,6A
R5689	T8865	T8863	cc	and,6A
R569	T1466	T1463	conj	BMP7,BMP2
R5690	T8866	T8863	conj	6B,6A
R5691	T8867	T8863	punct	),6A
R5692	T8868	T8835	punct	.,found
R5693	T8870	T8871	amod	Many,osteoblasts
R5694	T8871	T8872	nsubj	osteoblasts,line
R5695	T8873	T8874	det	the,bone
R5696	T8874	T8872	dobj	bone,line
R5697	T8875	T8876	advmod	newly,formed
R5698	T8876	T8874	amod	formed,bone
R5699	T8877	T8874	amod	cortical,bone
R57	T218	T217	pcomp	to,due
R570	T1467	T1464	advmod	thus,are
R5700	T8878	T8874	prep	in,bone
R5701	T8879	T8880	det	the,region
R5702	T8880	T8878	pobj	region,in
R5703	T8881	T8880	amod	diaphyseal,region
R5704	T8882	T8883	punct	(,arrow
R5705	T8883	T8872	parataxis	arrow,line
R5706	T8884	T8885	compound	Figure,6C
R5707	T8885	T8883	dep	6C,arrow
R5708	T8886	T8883	punct	", ",arrow
R5709	T8887	T8883	amod	blue,arrow
R571	T1468	T1469	amod	secondary,signals
R5710	T8888	T8883	punct	),arrow
R5711	T8889	T8872	punct	", ",line
R5712	T8890	T8872	cc	and,line
R5713	T8891	T8892	nsubj	formation,begun
R5714	T8892	T8872	conj	begun,line
R5715	T8893	T8891	prep	of,formation
R5716	T8894	T8895	det	a,center
R5717	T8895	T8893	pobj	center,of
R5718	T8896	T8895	amod	secondary,center
R5719	T8897	T8895	compound	ossification,center
R572	T1469	T1464	attr	signals,are
R5720	T8898	T8892	aux	has,begun
R5721	T8899	T8900	punct	(,star
R5722	T8900	T8892	parataxis	star,begun
R5723	T8901	T8902	compound	Figure,6A
R5724	T8902	T8900	dep	6A,star
R5725	T8903	T8900	punct	", ",star
R5726	T8904	T8900	amod	red,star
R5727	T8905	T8900	punct	),star
R5728	T8906	T8892	punct	.,begun
R5729	T8908	T8909	prep	At,is
R573	T1470	T1469	acl	produced,signals
R5730	T8910	T8911	nummod	3,wks
R5731	T8911	T8908	pobj	wks,At
R5732	T8912	T8911	prep	of,wks
R5733	T8913	T8912	pobj	age,of
R5734	T8914	T8909	punct	", ",is
R5735	T8915	T8909	nsubj	formation,is
R5736	T8916	T8915	prep	of,formation
R5737	T8917	T8918	det	the,center
R5738	T8918	T8916	pobj	center,of
R5739	T8919	T8918	amod	secondary,center
R574	T1471	T1470	prep	in,produced
R5740	T8920	T8918	compound	ossification,center
R5741	T8921	T8909	acomp	complete,is
R5742	T8922	T8923	punct	(,star
R5743	T8923	T8909	parataxis	star,is
R5744	T8924	T8925	compound	Figure,6D
R5745	T8925	T8923	dep	6D,star
R5746	T8926	T8923	punct	", ",star
R5747	T8927	T8923	amod	red,star
R5748	T8928	T8923	punct	),star
R5749	T8929	T8909	punct	", ",is
R575	T1472	T1471	pobj	response,in
R5750	T8930	T8909	prep	along,is
R5751	T8931	T8930	prep	with,along
R5752	T8932	T8933	amod	robust,formation
R5753	T8933	T8931	pobj	formation,with
R5754	T8934	T8935	compound	bone,marrow
R5755	T8935	T8933	compound	marrow,formation
R5756	T8936	T8937	punct	(,bm
R5757	T8937	T8933	parataxis	bm,formation
R5758	T8938	T8939	compound	Figure,6D
R5759	T8939	T8937	dep	6D,bm
R576	T1473	T1472	prep	to,response
R5760	T8940	T8937	punct	", ",bm
R5761	T8941	T8937	punct	),bm
R5762	T8942	T8933	cc	and,formation
R5763	T8943	T8933	conj	deposition,formation
R5764	T8944	T8943	prep	of,deposition
R5765	T8945	T8946	amod	trabecular,bone
R5766	T8946	T8944	pobj	bone,of
R5767	T8947	T8948	punct	(,tissue
R5768	T8948	T8909	parataxis	tissue,is
R5769	T8949	T8950	compound	Figure,6E
R577	T1474	T1475	compound	SHH,activity
R5770	T8950	T8948	dep	6E,tissue
R5771	T8951	T8948	punct	", ",tissue
R5772	T8952	T8948	amod	pale,tissue
R5773	T8953	T8948	amod	blue,tissue
R5774	T8954	T8948	acl	marked,tissue
R5775	T8955	T8954	agent	by,marked
R5776	T8956	T8957	amod	red,arrow
R5777	T8957	T8955	pobj	arrow,by
R5778	T8958	T8948	punct	),tissue
R5779	T8959	T8909	punct	.,is
R578	T1475	T1473	pobj	activity,to
R5780	T8961	T8962	prep	In,observed
R5781	T8963	T8964	amod	stark,contrast
R5782	T8964	T8961	pobj	contrast,In
R5783	T8965	T8962	punct	", ",observed
R5784	T8966	T8967	compound	bone,formation
R5785	T8967	T8962	nsubjpass	formation,observed
R5786	T8968	T8962	auxpass	is,observed
R5787	T8969	T8962	neg	not,observed
R5788	T8970	T8962	prep	in,observed
R5789	T8971	T8972	det	the,absence
R579	T1476	T1464	punct	.,are
R5790	T8972	T8970	pobj	absence,in
R5791	T8973	T8972	prep	of,absence
R5792	T8974	T8973	pobj	BMP2,of
R5793	T8975	T8974	cc	and,BMP2
R5794	T8976	T8974	conj	BMP4,BMP2
R5795	T8977	T8962	prep	at,observed
R5796	T8978	T8979	nummod	1,wks
R5797	T8979	T8977	pobj	wks,at
R5798	T8980	T8978	cc	or,1
R5799	T8981	T8978	conj	3,1
R58	T219	T217	pobj	complications,due
R580	T1478	T1479	advmod	Moreover,has
R5800	T8982	T8979	prep	after,wks
R5801	T8983	T8982	pobj	birth,after
R5802	T8984	T8985	punct	(,6F
R5803	T8985	T8962	parataxis	6F,observed
R5804	T8986	T8985	compound	Figure,6F
R5805	T8987	T8988	punct	–,6J
R5806	T8988	T8985	prep	6J,6F
R5807	T8989	T8985	punct	),6F
R5808	T8990	T8962	punct	.,observed
R5809	T8992	T8993	det	The,appearance
R581	T1480	T1479	punct	", ",has
R5810	T8993	T8994	nsubj	appearance,remains
R5811	T8995	T8993	prep	of,appearance
R5812	T8996	T8997	det	the,elements
R5813	T8997	T8995	pobj	elements,of
R5814	T8998	T8997	amod	skeletal,elements
R5815	T8999	T8993	prep	in,appearance
R5816	T9000	T9001	det	the,mutants
R5817	T9001	T8999	pobj	mutants,in
R5818	T9002	T9001	amod	double,mutants
R5819	T9003	T8994	acomp	similar,remains
R582	T1481	T1482	compound	BMP,signaling
R5820	T9004	T9003	prep	to,similar
R5821	T9005	T9006	compound	E17.5,structures
R5822	T9006	T9004	pobj	structures,to
R5823	T9007	T9006	acl	seen,structures
R5824	T9008	T9007	prep	in,seen
R5825	T9009	T9010	amod	wild,type
R5826	T9010	T9012	compound	type,limbs
R5827	T9011	T9010	punct	-,type
R5828	T9012	T9008	pobj	limbs,in
R5829	T9013	T9014	punct	(,5E
R583	T1482	T1479	nsubj	signaling,has
R5830	T9014	T9007	parataxis	5E,seen
R5831	T9015	T9014	compound	Figure,5E
R5832	T9016	T9014	punct	),5E
R5833	T9017	T8994	punct	", ",remains
R5834	T9018	T8994	cc	and,remains
R5835	T9019	T9020	mark	although,is
R5836	T9020	T9022	advcl	is,is
R5837	T9021	T9020	expl	there,is
R5838	T9022	T8994	conj	is,remains
R5839	T9023	T9024	det	a,collar
R584	T1483	T1484	det	a,ability
R5840	T9024	T9020	attr	collar,is
R5841	T9025	T9024	compound	bone,collar
R5842	T9026	T9020	prep	at,is
R5843	T9027	T9028	det	the,site
R5844	T9028	T9026	pobj	site,at
R5845	T9029	T9028	prep	of,site
R5846	T9030	T9031	amod	hypertrophic,cartilage
R5847	T9031	T9029	pobj	cartilage,of
R5848	T9032	T9033	punct	(,6G
R5849	T9033	T9020	parataxis	6G,is
R585	T1484	T1479	dobj	ability,has
R5850	T9034	T9033	compound	Figure,6G
R5851	T9035	T9033	punct	),6G
R5852	T9036	T9022	punct	", ",is
R5853	T9037	T9038	det	no,cavity
R5854	T9038	T9022	nsubj	cavity,is
R5855	T9039	T9040	compound	bone,marrow
R5856	T9040	T9038	compound	marrow,cavity
R5857	T9041	T9038	punct	", ",cavity
R5858	T9042	T9043	amod	trabecular,bone
R5859	T9043	T9038	conj	bone,cavity
R586	T1485	T1484	amod	weak,ability
R5860	T9044	T9043	punct	", ",bone
R5861	T9045	T9043	cc	or,bone
R5862	T9046	T9047	amod	cortical,bone
R5863	T9047	T9043	conj	bone,bone
R5864	T9048	T9022	acomp	present,is
R5865	T9049	T9050	punct	(,6F
R5866	T9050	T9022	parataxis	6F,is
R5867	T9051	T9050	compound	Figure,6F
R5868	T9052	T9050	punct	),6F
R5869	T9053	T9022	punct	.,is
R587	T1486	T1487	aux	to,polarize
R5870	T9055	T9056	amod	Many,osteoclasts
R5871	T9056	T9057	nsubjpass	osteoclasts,recruited
R5872	T9058	T9057	aux	have,recruited
R5873	T9059	T9057	auxpass	been,recruited
R5874	T9060	T9057	prep	to,recruited
R5875	T9061	T9062	det	the,cartilage
R5876	T9062	T9060	pobj	cartilage,to
R5877	T9063	T9062	amod	remaining,cartilage
R5878	T9064	T9062	amod	mineralized,cartilage
R5879	T9065	T9057	cc	and,recruited
R588	T1487	T1484	acl	polarize,ability
R5880	T9066	T9067	aux	are,resorbing
R5881	T9067	T9057	conj	resorbing,recruited
R5882	T9068	T9067	advmod	actively,resorbing
R5883	T9069	T9070	det	this,tissue
R5884	T9070	T9067	dobj	tissue,resorbing
R5885	T9071	T9072	punct	(,6I
R5886	T9072	T9067	parataxis	6I,resorbing
R5887	T9073	T9074	amod	red,asterisks
R5888	T9074	T9072	dep	asterisks,6I
R5889	T9075	T9072	punct	", ",6I
R589	T1488	T1487	advmod	posteriorly,polarize
R5890	T9076	T9072	compound	Figure,6I
R5891	T9077	T9072	punct	),6I
R5892	T9078	T9057	punct	", ",recruited
R5893	T9079	T9080	mark	so,disappeared
R5894	T9080	T9057	advcl	disappeared,recruited
R5895	T9081	T9080	mark	that,disappeared
R5896	T9082	T9080	prep	by,disappeared
R5897	T9083	T9084	nummod	3,wks
R5898	T9084	T9082	pobj	wks,by
R5899	T9085	T9080	punct	", ",disappeared
R59	T220	T219	acl	associated,complications
R590	T1489	T1490	det	the,limb
R5900	T9086	T9087	predet	all,cartilage
R5901	T9087	T9080	nsubj	cartilage,disappeared
R5902	T9088	T9087	det	the,cartilage
R5903	T9089	T9087	amod	mineralized,cartilage
R5904	T9090	T9087	prep	in,cartilage
R5905	T9091	T9092	det	the,region
R5906	T9092	T9090	pobj	region,in
R5907	T9093	T9092	amod	diaphyseal,region
R5908	T9094	T9080	aux	has,disappeared
R5909	T9095	T9080	punct	", ",disappeared
R591	T1490	T1487	dobj	limb,polarize
R5910	T9096	T9080	advcl	leaving,disappeared
R5911	T9097	T9098	det	a,void
R5912	T9098	T9096	dobj	void,leaving
R5913	T9099	T9100	advmod	where,occurred
R5914	T9100	T9098	relcl	occurred,void
R5915	T9101	T9102	compound	bone,formation
R5916	T9102	T9100	nsubj	formation,occurred
R5917	T9103	T9100	aux	should,occurred
R5918	T9104	T9100	aux	have,occurred
R5919	T9105	T9106	punct	(,6J
R592	T1491	T1487	prep	in,polarize
R5920	T9106	T9057	parataxis	6J,recruited
R5921	T9107	T9106	compound	Figure,6J
R5922	T9108	T9106	punct	", ",6J
R5923	T9109	T9110	advmod	also,see
R5924	T9110	T9106	advcl	see,6J
R5925	T9111	T9112	compound	Figures,6L
R5926	T9112	T9110	dobj	6L,see
R5927	T9113	T9112	cc	and,6L
R5928	T9114	T9112	conj	S3,6L
R5929	T9115	T9106	punct	),6J
R593	T1492	T1493	amod	ectopic,experiments
R5930	T9116	T9057	punct	.,recruited
R5931	T9118	T9119	det	This,void
R5932	T9119	T9120	nsubjpass	void,invaded
R5933	T9121	T9120	auxpass	is,invaded
R5934	T9122	T9120	advmod	eventually,invaded
R5935	T9123	T9120	agent	by,invaded
R5936	T9124	T9125	amod	soft,tissues
R5937	T9125	T9123	pobj	tissues,by
R5938	T9126	T9125	cc	and,tissues
R5939	T9127	T9128	det	the,muscle
R594	T1493	T1491	pobj	experiments,in
R5940	T9128	T9125	conj	muscle,tissues
R5941	T9129	T9128	amod	adjacent,muscle
R5942	T9130	T9128	punct	(,muscle
R5943	T9131	T9128	acl	marked,muscle
R5944	T9132	T9131	oprd	s,marked
R5945	T9133	T9132	cc	and,s
R5946	T9134	T9132	conj	m,s
R5947	T9135	T9131	punct	", ",marked
R5948	T9136	T9131	advmod	respectively,marked
R5949	T9137	T9131	punct	", ",marked
R595	T1494	T1493	compound	grafting,experiments
R5950	T9138	T9131	prep	in,marked
R5951	T9139	T9140	compound	Figure,6J
R5952	T9140	T9138	pobj	6J,in
R5953	T9141	T9120	punct	),invaded
R5954	T9142	T9120	punct	.,invaded
R5955	T9144	T9145	det	The,resorption
R5956	T9145	T9146	nsubj	resorption,is
R5957	T9147	T9145	prep	of,resorption
R5958	T9148	T9147	pobj	bone,of
R5959	T9149	T9145	prep	at,resorption
R596	T1495	T1496	punct	[,10
R5960	T9150	T9151	nummod	3,wks
R5961	T9151	T9149	pobj	wks,at
R5962	T9152	T9151	prep	of,wks
R5963	T9153	T9152	pobj	age,of
R5964	T9154	T9155	advmod	clearly,visible
R5965	T9155	T9146	acomp	visible,is
R5966	T9156	T9146	prep	in,is
R5967	T9157	T9158	det	the,femur
R5968	T9158	T9156	pobj	femur,in
R5969	T9159	T9158	amod	proximal,femur
R597	T1496	T1484	parataxis	10,ability
R5970	T9160	T9158	prep	of,femur
R5971	T9161	T9162	compound	mutant,animals
R5972	T9162	T9160	pobj	animals,of
R5973	T9163	T9164	advmod	where,is
R5974	T9164	T9158	relcl	is,femur
R5975	T9165	T9166	det	a,portion
R5976	T9166	T9164	nsubj	portion,is
R5977	T9167	T9166	prep	of,portion
R5978	T9168	T9169	det	that,bone
R5979	T9169	T9167	pobj	bone,of
R598	T1497	T1496	punct	],10
R5980	T9170	T9164	acomp	missing,is
R5981	T9171	T9172	punct	(,arrow
R5982	T9172	T9146	parataxis	arrow,is
R5983	T9173	T9174	compound	Figure,6L
R5984	T9174	T9172	dep	6L,arrow
R5985	T9175	T9172	punct	", ",arrow
R5986	T9176	T9172	amod	black,arrow
R5987	T9177	T9172	punct	),arrow
R5988	T9178	T9146	punct	.,is
R5989	T9180	T9181	det	The,inability
R599	T1498	T1484	punct	", ",ability
R5990	T9181	T9182	nsubj	inability,results
R5991	T9183	T9184	aux	to,complete
R5992	T9184	T9181	acl	complete,inability
R5993	T9185	T9186	compound	osteoblast,differentiation
R5994	T9186	T9184	dobj	differentiation,complete
R5995	T9187	T9184	prep	in,complete
R5996	T9188	T9189	det	the,mice
R5997	T9189	T9187	pobj	mice,in
R5998	T9190	T9191	nmod	Bmp2C,C
R5999	T9191	T9189	nmod	C,mice
R6	T160	T159	punct	", ",BMP2
R60	T221	T220	prep	with,associated
R600	T1499	T1500	det	an,activity
R6000	T9192	T9191	punct	/,C
R6001	T9193	T9191	punct	;,C
R6002	T9194	T9195	compound	Bmp4C,C
R6003	T9195	T9191	appos	C,C
R6004	T9196	T9195	punct	/,C
R6005	T9197	T9191	punct	;,C
R6006	T9198	T9199	compound	Prx1,cre
R6007	T9199	T9191	appos	cre,C
R6008	T9200	T9199	punct	:,cre
R6009	T9201	T9199	punct	:,cre
R601	T1500	T1484	appos	activity,ability
R6010	T9202	T9182	prep	in,results
R6011	T9203	T9204	advmod	strikingly,defective
R6012	T9204	T9205	amod	defective,morphology
R6013	T9205	T9202	pobj	morphology,in
R6014	T9206	T9205	compound	limb,morphology
R6015	T9207	T9205	prep	at,morphology
R6016	T9208	T9209	nummod	1,wk
R6017	T9209	T9207	pobj	wk,at
R6018	T9210	T9209	prep	of,wk
R6019	T9211	T9210	pobj	age,of
R602	T1501	T1500	acl	enhanced,activity
R6020	T9212	T9213	punct	(,compare
R6021	T9213	T9182	parataxis	compare,results
R6022	T9214	T9215	compound	Figure,S3G
R6023	T9215	T9213	dobj	S3G,compare
R6024	T9216	T9215	cc	and,S3G
R6025	T9217	T9215	conj	S3H,S3G
R6026	T9218	T9213	punct	),compare
R6027	T9219	T9182	punct	.,results
R6028	T9221	T9222	aux	To,determine
R6029	T9222	T9223	advcl	determine,examined
R603	T1502	T1501	prep	by,enhanced
R6030	T9224	T9225	mark	if,occurs
R6031	T9225	T9222	ccomp	occurs,determine
R6032	T9226	T9227	det	any,differentiation
R6033	T9227	T9225	nsubj	differentiation,occurs
R6034	T9228	T9227	compound	osteoblast,differentiation
R6035	T9229	T9225	prep	in,occurs
R6036	T9230	T9231	nmod	Bmp2C,C
R6037	T9231	T9233	nmod	C,forelimbs
R6038	T9232	T9231	punct	/,C
R6039	T9233	T9229	pobj	forelimbs,in
R604	T1503	T1504	amod	prior,exposure
R6040	T9234	T9231	punct	;,C
R6041	T9235	T9236	compound	Bmp4C,C
R6042	T9236	T9231	appos	C,C
R6043	T9237	T9236	punct	/,C
R6044	T9238	T9231	punct	;,C
R6045	T9239	T9240	compound	Prx1,cre
R6046	T9240	T9231	appos	cre,C
R6047	T9241	T9240	punct	:,cre
R6048	T9242	T9240	punct	:,cre
R6049	T9243	T9223	punct	", ",examined
R605	T1504	T1502	pobj	exposure,by
R6050	T9244	T9223	nsubj	we,examined
R6051	T9245	T9246	npadvmod	osteoblast,related
R6052	T9246	T9248	amod	related,expression
R6053	T9247	T9246	punct	-,related
R6054	T9248	T9223	dobj	expression,examined
R6055	T9249	T9248	compound	gene,expression
R6056	T9250	T9251	punct	(,Figure
R6057	T9251	T9223	parataxis	Figure,examined
R6058	T9252	T9251	nummod	7,Figure
R6059	T9253	T9251	punct	),Figure
R606	T1505	T1506	amod	low,level
R6060	T9254	T9223	punct	.,examined
R6061	T9256	T9257	prep	In,express
R6062	T9258	T9259	det	the,mice
R6063	T9259	T9256	pobj	mice,In
R6064	T9260	T9259	compound	control,mice
R6065	T9261	T9257	punct	", ",express
R6066	T9262	T9257	nsubj	osteoblasts,express
R6067	T9263	T9262	acl	lining,osteoblasts
R6068	T9264	T9265	det	the,surface
R6069	T9265	T9263	dobj	surface,lining
R607	T1506	T1504	compound	level,exposure
R6070	T9266	T9265	prep	of,surface
R6071	T9267	T9268	amod	trabecular,bone
R6072	T9268	T9266	pobj	bone,of
R6073	T9269	T9267	cc	and,trabecular
R6074	T9270	T9267	conj	cortical,trabecular
R6075	T9271	T9272	nmod	type,collagen
R6076	T9272	T9257	dobj	collagen,express
R6077	T9273	T9271	nummod	I,type
R6078	T9274	T9272	punct	", ",collagen
R6079	T9275	T9272	conj	runx2,collagen
R608	T1507	T1506	punct	-,level
R6080	T9276	T9275	punct	", ",runx2
R6081	T9277	T9275	cc	and,runx2
R6082	T9278	T9279	compound	osterix,genes
R6083	T9279	T9275	conj	genes,runx2
R6084	T9280	T9257	prep	at,express
R6085	T9281	T9282	preconj	both,wk
R6086	T9282	T9280	pobj	wk,at
R6087	T9283	T9282	nummod	1,wk
R6088	T9284	T9282	cc	and,wk
R6089	T9285	T9286	nummod	3,wks
R609	T1508	T1504	prep	to,exposure
R6090	T9286	T9282	conj	wks,wk
R6091	T9287	T9282	prep	after,wk
R6092	T9288	T9287	pobj	birth,after
R6093	T9289	T9290	punct	(,7A
R6094	T9290	T9257	parataxis	7A,express
R6095	T9291	T9290	compound	Figure,7A
R6096	T9292	T9293	punct	–,7D
R6097	T9293	T9290	prep	7D,7A
R6098	T9294	T9290	cc	and,7A
R6099	T9295	T9290	conj	7I,7A
R61	T222	T223	amod	early,lethality
R610	T1509	T1508	pobj	SHH,to
R6100	T9296	T9297	punct	–,7L
R6101	T9297	T9295	prep	7L,7I
R6102	T9298	T9290	punct	),7A
R6103	T9299	T9257	punct	.,express
R6104	T9301	T9302	prep	In,found
R6105	T9303	T9304	det	the,absence
R6106	T9304	T9301	pobj	absence,In
R6107	T9305	T9304	prep	of,absence
R6108	T9306	T9305	pobj	BMP2,of
R6109	T9307	T9306	cc	and,BMP2
R611	T1510	T1511	punct	[,11
R6110	T9308	T9306	conj	BMP4,BMP2
R6111	T9309	T9302	punct	", ",found
R6112	T9310	T9302	nsubjpass	cells,found
R6113	T9311	T9312	dep	which,are
R6114	T9312	T9310	relcl	are,cells
R6115	T9313	T9312	acomp	fibroblastic,are
R6116	T9314	T9312	prep	in,are
R6117	T9315	T9314	pobj	appearance,in
R6118	T9316	T9317	punct	(,6H
R6119	T9317	T9310	parataxis	6H,cells
R612	T1511	T1500	parataxis	11,activity
R6120	T9318	T9317	compound	Figure,6H
R6121	T9319	T9317	punct	),6H
R6122	T9320	T9302	auxpass	are,found
R6123	T9321	T9302	oprd	adjacent,found
R6124	T9322	T9321	prep	to,adjacent
R6125	T9323	T9324	det	the,surfaces
R6126	T9324	T9322	pobj	surfaces,to
R6127	T9325	T9324	amod	mineralized,surfaces
R6128	T9326	T9324	compound	cartilage,surfaces
R6129	T9327	T9302	punct	.,found
R613	T1512	T1511	punct	],11
R6130	T9329	T9330	det	These,cells
R6131	T9330	T9331	nsubj	cells,express
R6132	T9332	T9331	dobj	amounts,express
R6133	T9333	T9332	prep	of,amounts
R6134	T9334	T9335	nmod	type,collagen
R6135	T9335	T9333	pobj	collagen,of
R6136	T9336	T9334	nummod	I,type
R6137	T9337	T9335	cc	and,collagen
R6138	T9338	T9335	conj	runx2,collagen
R6139	T9339	T9332	amod	comparable,amounts
R614	T1513	T1479	punct	.,has
R6140	T9340	T9339	prep	to,comparable
R6141	T9341	T9342	compound	control,osteoblasts
R6142	T9342	T9340	pobj	osteoblasts,to
R6143	T9343	T9331	punct	", ",express
R6144	T9344	T9331	cc	but,express
R6145	T9345	T9346	poss	their,expression
R6146	T9346	T9347	nsubj	expression,becomes
R6147	T9347	T9331	conj	becomes,express
R6148	T9348	T9346	prep	of,expression
R6149	T9349	T9348	pobj	osterix,of
R615	T1515	T1516	nsubj	It,remains
R6150	T9350	T9347	punct	", ",becomes
R6151	T9351	T9352	mark	while,noticeable
R6152	T9352	T9347	advcl	noticeable,becomes
R6153	T9353	T9352	prep	at,noticeable
R6154	T9354	T9355	nummod	1,wk
R6155	T9355	T9353	pobj	wk,at
R6156	T9356	T9347	punct	", ",becomes
R6157	T9357	T9358	advmod	almost,undetectable
R6158	T9358	T9347	acomp	undetectable,becomes
R6159	T9359	T9347	prep	at,becomes
R616	T1517	T1516	acomp	unclear,remains
R6160	T9360	T9361	nummod	3,wks
R6161	T9361	T9359	pobj	wks,at
R6162	T9362	T9363	punct	(,7E
R6163	T9363	T9347	parataxis	7E,becomes
R6164	T9364	T9363	compound	Figure,7E
R6165	T9365	T9366	punct	–,7H
R6166	T9366	T9363	prep	7H,7E
R6167	T9367	T9363	cc	and,7E
R6168	T9368	T9363	conj	7M,7E
R6169	T9369	T9370	punct	–,7P
R617	T1518	T1516	punct	", ",remains
R6170	T9370	T9368	prep	7P,7M
R6171	T9371	T9363	punct	),7E
R6172	T9372	T9347	punct	.,becomes
R6173	T9374	T9375	det	These,results
R6174	T9375	T9376	nsubj	results,allow
R6175	T9377	T9378	nsubj	us,conclude
R6176	T9378	T9376	ccomp	conclude,allow
R6177	T9379	T9378	aux	to,conclude
R6178	T9380	T9381	mark	that,be
R6179	T9381	T9378	ccomp	be,conclude
R618	T1519	T1516	advmod	however,remains
R6180	T9382	T9383	det	the,cells
R6181	T9383	T9381	nsubj	cells,be
R6182	T9384	T9385	npadvmod	fibroblast,like
R6183	T9385	T9383	amod	like,cells
R6184	T9386	T9385	punct	-,like
R6185	T9387	T9383	amod	resident,cells
R6186	T9388	T9387	prep	in,resident
R6187	T9389	T9390	det	the,shaft
R6188	T9390	T9388	pobj	shaft,in
R6189	T9391	T9390	compound	bone,shaft
R619	T1520	T1516	punct	", ",remains
R6190	T9392	T9393	punct	(,6H
R6191	T9393	T9383	parataxis	6H,cells
R6192	T9394	T9393	compound	Figure,6H
R6193	T9395	T9393	punct	),6H
R6194	T9396	T9381	aux	may,be
R6195	T9397	T9398	compound	osteoprogenitor,cells
R6196	T9398	T9381	attr	cells,be
R6197	T9399	T9398	amod	unable,cells
R6198	T9400	T9401	aux	to,differentiate
R6199	T9401	T9399	xcomp	differentiate,unable
R62	T223	T221	pobj	lethality,with
R620	T1521	T1522	mark	whether,required
R6200	T9402	T9401	prep	into,differentiate
R6201	T9403	T9404	amod	mature,osteoblasts
R6202	T9404	T9402	pobj	osteoblasts,into
R6203	T9405	T9401	prep	in,differentiate
R6204	T9406	T9407	det	the,absence
R6205	T9407	T9405	pobj	absence,in
R6206	T9408	T9407	prep	of,absence
R6207	T9409	T9408	pobj	BMP2,of
R6208	T9410	T9409	cc	and,BMP2
R6209	T9411	T9409	conj	BMP4,BMP2
R621	T1522	T1516	ccomp	required,remains
R6210	T9412	T9376	punct	.,allow
R6211	T9476	T9477	advcl	Using,found
R6212	T9478	T9479	det	a,approach
R6213	T9479	T9476	dobj	approach,Using
R6214	T9480	T9479	amod	genetic,approach
R6215	T9481	T9482	aux	to,remove
R6216	T9482	T9476	advcl	remove,Using
R6217	T9483	T9482	dobj	activity,remove
R6218	T9484	T9483	prep	of,activity
R6219	T9485	T9486	amod	different,genes
R622	T1523	T1524	nmod	BMP2,activity
R6220	T9486	T9484	pobj	genes,of
R6221	T9487	T9486	compound	Bmp,genes
R6222	T9488	T9482	prep	during,remove
R6223	T9489	T9490	compound	limb,development
R6224	T9490	T9488	pobj	development,during
R6225	T9491	T9477	punct	", ",found
R6226	T9492	T9477	nsubj	we,found
R6227	T9493	T9477	aux	have,found
R6228	T9494	T9495	mark	that,required
R6229	T9495	T9477	ccomp	required,found
R623	T1524	T1522	nsubjpass	activity,required
R6230	T9496	T9495	nsubjpass	BMP2,required
R6231	T9497	T9496	punct	", ",BMP2
R6232	T9498	T9496	conj	BMP4,BMP2
R6233	T9499	T9498	punct	", ",BMP4
R6234	T9500	T9498	cc	and,BMP4
R6235	T9501	T9498	conj	BMP7,BMP4
R6236	T9502	T9503	preconj	either,in
R6237	T9503	T9495	prep	in,required
R6238	T9504	T9503	advmod	individually,in
R6239	T9505	T9503	cc	or,in
R624	T1525	T1523	cc	and,BMP2
R6240	T9506	T9503	pobj	combination,in
R6241	T9507	T9495	auxpass	are,required
R6242	T9508	T9495	neg	not,required
R6243	T9509	T9495	prep	for,required
R6244	T9510	T9509	pobj	specification,for
R6245	T9511	T9510	prep	of,specification
R6246	T9512	T9513	amod	normal,identities
R6247	T9513	T9511	pobj	identities,of
R6248	T9514	T9513	compound	digit,identities
R6249	T9515	T9477	punct	.,found
R625	T1526	T1523	conj	BMP7,BMP2
R6250	T9517	T9518	advmod	However,required
R6251	T9519	T9518	punct	", ",required
R6252	T9520	T9518	nsubjpass	BMP2,required
R6253	T9521	T9520	cc	and,BMP2
R6254	T9522	T9520	conj	BMP4,BMP2
R6255	T9523	T9518	auxpass	are,required
R6256	T9524	T9518	prep	in,required
R6257	T9525	T9524	pobj	concert,in
R6258	T9526	T9527	aux	to,promote
R6259	T9527	T9518	advcl	promote,required
R626	T1527	T1522	auxpass	is,required
R6260	T9528	T9527	dobj	condensation,promote
R6261	T9529	T9528	prep	of,condensation
R6262	T9530	T9531	det	the,anlagen
R6263	T9531	T9529	pobj	anlagen,of
R6264	T9532	T9531	amod	posterior,anlagen
R6265	T9533	T9531	compound	digit,anlagen
R6266	T9534	T9518	punct	.,required
R6267	T9536	T9537	nsubj	We,shown
R6268	T9538	T9537	aux	have,shown
R6269	T9539	T9537	advmod	also,shown
R627	T1528	T1522	advmod	endogenously,required
R6270	T9540	T9541	mark	that,initiated
R6271	T9541	T9537	ccomp	initiated,shown
R6272	T9542	T9541	nsubjpass	chondrogenesis,initiated
R6273	T9543	T9541	aux	can,initiated
R6274	T9544	T9541	auxpass	be,initiated
R6275	T9545	T9541	cc	and,initiated
R6276	T9546	T9547	amod	chondrogenic,differentiation
R6277	T9547	T9548	nsubj	differentiation,take
R6278	T9548	T9541	conj	take,initiated
R6279	T9549	T9548	aux	will,take
R628	T1529	T1522	prep	for,required
R6280	T9550	T9548	dobj	place,take
R6281	T9551	T9552	advmod	even,in
R6282	T9552	T9548	prep	in,take
R6283	T9553	T9554	det	the,absence
R6284	T9554	T9552	pobj	absence,in
R6285	T9555	T9554	prep	of,absence
R6286	T9556	T9557	preconj	both,BMP2
R6287	T9557	T9555	pobj	BMP2,of
R6288	T9558	T9557	cc	and,BMP2
R6289	T9559	T9557	conj	BMP4,BMP2
R629	T1530	T1531	amod	anterior,posterior
R6290	T9560	T9557	cc	or,BMP2
R6291	T9561	T9557	conj	BMP2,BMP2
R6292	T9562	T9561	cc	and,BMP2
R6293	T9563	T9561	conj	BMP7,BMP2
R6294	T9564	T9537	punct	.,shown
R6295	T9566	T9567	prep	On,required
R6296	T9568	T9569	det	the,hand
R6297	T9569	T9566	pobj	hand,On
R6298	T9570	T9569	amod	other,hand
R6299	T9571	T9567	punct	", ",required
R63	T224	T223	amod	embryonic,lethality
R630	T1531	T1533	amod	posterior,patterning
R6300	T9572	T9567	nsubjpass	BMP2,required
R6301	T9573	T9572	cc	and,BMP2
R6302	T9574	T9572	conj	BMP4,BMP2
R6303	T9575	T9567	advmod	together,required
R6304	T9576	T9567	auxpass	are,required
R6305	T9577	T9567	prep	for,required
R6306	T9578	T9577	pobj	completion,for
R6307	T9579	T9578	prep	of,completion
R6308	T9580	T9579	pobj	osteogenesis,of
R6309	T9581	T9567	punct	.,required
R631	T1532	T1531	punct	-,posterior
R6314	T10021	T10022	compound	BMP,Signaling
R6315	T10023	T10022	cc	and,Signaling
R6316	T10024	T10025	compound	Pattern,Formation
R6317	T10025	T10022	conj	Formation,Signaling
R6318	T10027	T10028	nsubjpass	It,proposed
R6319	T10029	T10028	aux	has,proposed
R632	T1533	T1529	pobj	patterning,for
R6320	T10030	T10028	auxpass	been,proposed
R6321	T10031	T10032	mark	that,involved
R6322	T10032	T10028	ccomp	involved,proposed
R6323	T10033	T10034	compound	BMP,signaling
R6324	T10034	T10032	nsubjpass	signaling,involved
R6325	T10035	T10032	aux	may,involved
R6326	T10036	T10032	auxpass	be,involved
R6327	T10037	T10032	prep	in,involved
R6328	T10038	T10039	compound	digit,patterning
R6329	T10039	T10037	pobj	patterning,in
R633	T1534	T1533	compound	limb,patterning
R6330	T10040	T10032	prep	as,involved
R6331	T10041	T10042	det	a,signal
R6332	T10042	T10040	pobj	signal,as
R6333	T10043	T10042	amod	secondary,signal
R6334	T10044	T10042	acl	originating,signal
R6335	T10045	T10044	prep	in,originating
R6336	T10046	T10047	det	the,ZPA
R6337	T10047	T10045	pobj	ZPA,in
R6338	T10048	T10049	punct	[,43
R6339	T10049	T10047	parataxis	43,ZPA
R634	T1535	T1533	prep	by,patterning
R6340	T10050	T10049	nummod	7,43
R6341	T10051	T10049	punct	",",43
R6342	T10052	T10049	nummod	11,43
R6343	T10053	T10049	punct	",",43
R6344	T10054	T10049	punct	],43
R6345	T10055	T10040	cc	or,as
R6346	T10056	T10040	conj	as,as
R6347	T10057	T10058	det	a,mediator
R6348	T10058	T10056	pobj	mediator,as
R6349	T10059	T10058	amod	later,mediator
R635	T1536	T1535	pobj	SHH,by
R6350	T10060	T10058	prep	of,mediator
R6351	T10061	T10062	amod	interdigital,information
R6352	T10062	T10060	pobj	information,of
R6353	T10063	T10062	compound	patterning,information
R6354	T10064	T10065	punct	[,15
R6355	T10065	T10058	parataxis	15,mediator
R6356	T10066	T10065	punct	],15
R6357	T10067	T10028	punct	.,proposed
R6358	T10069	T10070	prep	In,suggested
R6359	T10071	T10069	amod	particular,In
R636	T1537	T1516	punct	.,remains
R6360	T10072	T10070	punct	", ",suggested
R6361	T10073	T10070	nsubjpass	BMP2,suggested
R6362	T10074	T10073	punct	", ",BMP2
R6363	T10075	T10076	det	a,factor
R6364	T10076	T10073	appos	factor,BMP2
R6365	T10077	T10076	amod	secreted,factor
R6366	T10078	T10079	dep	that,induced
R6367	T10079	T10076	relcl	induced,factor
R6368	T10080	T10079	auxpass	is,induced
R6369	T10081	T10079	agent	by,induced
R637	T1539	T1540	compound	Bmp2,embryos
R6370	T10082	T10081	pobj	SHH,by
R6371	T10083	T10084	punct	[,8
R6372	T10084	T10082	parataxis	8,SHH
R6373	T10085	T10084	punct	],8
R6374	T10086	T10079	prep	in,induced
R6375	T10087	T10088	det	the,bud
R6376	T10088	T10086	pobj	bud,in
R6377	T10089	T10088	amod	posterior,bud
R6378	T10090	T10088	compound	limb,bud
R6379	T10091	T10070	punct	", ",suggested
R638	T1540	T1542	nsubj	embryos,die
R6380	T10092	T10070	aux	has,suggested
R6381	T10093	T10070	auxpass	been,suggested
R6382	T10094	T10095	aux	to,act
R6383	T10095	T10070	xcomp	act,suggested
R6384	T10096	T10095	prep	as,act
R6385	T10097	T10098	det	a,morphogen
R6386	T10098	T10096	pobj	morphogen,as
R6387	T10099	T10098	amod	true,morphogen
R6388	T10100	T10098	amod	secondary,morphogen
R6389	T10101	T10100	prep	to,secondary
R639	T1541	T1540	compound	mutant,embryos
R6390	T10102	T10103	compound	SHH,signaling
R6391	T10103	T10101	pobj	signaling,to
R6392	T10104	T10105	aux	to,pattern
R6393	T10105	T10095	advcl	pattern,act
R6394	T10106	T10105	advmod	properly,pattern
R6395	T10107	T10108	det	the,digits
R6396	T10108	T10105	dobj	digits,pattern
R6397	T10109	T10108	compound	limb,digits
R6398	T10110	T10070	punct	.,suggested
R6399	T10112	T10113	advmod	However,cause
R64	T225	T223	punct	", ",lethality
R640	T1543	T1544	advmod	too,early
R6400	T10114	T10113	punct	", ",cause
R6401	T10115	T10113	nsubj	inactivation,cause
R6402	T10116	T10115	prep	of,inactivation
R6403	T10117	T10116	pobj	Bmp2,of
R6404	T10118	T10115	prep	during,inactivation
R6405	T10119	T10120	amod	early,development
R6406	T10120	T10118	pobj	development,during
R6407	T10121	T10122	compound	limb,bud
R6408	T10122	T10120	compound	bud,development
R6409	T10123	T10113	advmod	clearly,cause
R641	T1544	T1542	advmod	early,die
R6410	T10124	T10113	aux	does,cause
R6411	T10125	T10113	neg	not,cause
R6412	T10126	T10127	det	any,defects
R6413	T10127	T10113	dobj	defects,cause
R6414	T10128	T10129	compound	digit,patterning
R6415	T10129	T10127	compound	patterning,defects
R6416	T10130	T10113	punct	.,cause
R6417	T10132	T10133	nsubjpass	BMPs,known
R6418	T10134	T10133	auxpass	are,known
R6419	T10135	T10133	punct	", ",known
R642	T1545	T1546	aux	to,assess
R6420	T10136	T10133	prep	in,known
R6421	T10137	T10138	amod	many,cases
R6422	T10138	T10136	pobj	cases,in
R6423	T10139	T10133	punct	", ",known
R6424	T10140	T10141	aux	to,function
R6425	T10141	T10133	xcomp	function,known
R6426	T10142	T10141	advmod	redundantly,function
R6427	T10143	T10144	punct	[,44
R6428	T10144	T10133	parataxis	44,known
R6429	T10145	T10144	punct	],44
R643	T1546	T1544	xcomp	assess,early
R6430	T10146	T10133	punct	.,known
R6431	T10148	T10149	prep	In,is
R6432	T10150	T10148	pcomp	considering,In
R6433	T10151	T10152	amod	potential,signals
R6434	T10152	T10150	dobj	signals,considering
R6435	T10153	T10152	amod	secondary,signals
R6436	T10154	T10152	acl	mediating,signals
R6437	T10155	T10156	compound	ZPA,activity
R6438	T10156	T10154	dobj	activity,mediating
R6439	T10157	T10149	punct	", ",is
R644	T1547	T1548	poss	their,phenotypes
R6440	T10158	T10159	det	the,member
R6441	T10159	T10149	nsubj	member,is
R6442	T10160	T10161	advmod	most,relevant
R6443	T10161	T10159	amod	relevant,member
R6444	T10162	T10159	compound	family,member
R6445	T10163	T10164	aux	to,consider
R6446	T10164	T10159	advcl	consider,member
R6447	T10165	T10149	attr	BMP7,is
R6448	T10166	T10167	mark	as,regulated
R6449	T10167	T10149	advcl	regulated,is
R645	T1548	T1546	dobj	phenotypes,assess
R6450	T10168	T10167	punct	", ",regulated
R6451	T10169	T10167	prep	like,regulated
R6452	T10170	T10169	pobj	BMP2,like
R6453	T10171	T10169	punct	(,like
R6454	T10172	T10169	cc	and,like
R6455	T10173	T10169	conj	unlike,like
R6456	T10174	T10173	pobj	BMP4,unlike
R6457	T10175	T10167	punct	),regulated
R6458	T10176	T10167	punct	", ",regulated
R6459	T10177	T10167	nsubjpass	it,regulated
R646	T1549	T1548	compound	limb,phenotypes
R6460	T10178	T10167	auxpass	is,regulated
R6461	T10179	T10167	advmod	positively,regulated
R6462	T10180	T10167	prep	by,regulated
R6463	T10181	T10182	compound	SHH,signaling
R6464	T10182	T10180	pobj	signaling,by
R6465	T10183	T10184	punct	[,7
R6466	T10184	T10149	parataxis	7,is
R6467	T10185	T10184	punct	],7
R6468	T10186	T10149	punct	.,is
R6469	T10188	T10189	advmod	However,overlap
R647	T1550	T1542	punct	.,die
R6470	T10190	T10189	punct	", ",overlap
R6471	T10191	T10192	compound	Bmp7,expression
R6472	T10192	T10189	nsubj	expression,overlap
R6473	T10193	T10189	aux	does,overlap
R6474	T10194	T10189	neg	not,overlap
R6475	T10195	T10189	prep	with,overlap
R6476	T10196	T10195	pobj	Bmp2,with
R6477	T10197	T10189	prep	in,overlap
R6478	T10198	T10199	det	the,bud
R6479	T10199	T10197	pobj	bud,in
R648	T1552	T1553	det	A,deletion
R6480	T10200	T10199	amod	early,bud
R6481	T10201	T10199	compound	mouse,bud
R6482	T10202	T10199	compound	limb,bud
R6483	T10203	T10204	punct	[,45
R6484	T10204	T10189	parataxis	45,overlap
R6485	T10205	T10204	punct	],45
R6486	T10206	T10189	punct	", ",overlap
R6487	T10207	T10189	cc	and,overlap
R6488	T10208	T10209	prep	in,show
R6489	T10209	T10189	conj	show,overlap
R649	T1553	T1555	nsubjpass	deletion,made
R6490	T10210	T10211	det	any,case
R6491	T10211	T10208	pobj	case,in
R6492	T10212	T10209	punct	", ",show
R6493	T10213	T10209	nsubj	mice,show
R6494	T10214	T10215	prep	in,knocked
R6495	T10215	T10213	relcl	knocked,mice
R6496	T10216	T10214	pobj	which,in
R6497	T10217	T10215	nsubj	we,knocked
R6498	T10218	T10215	advmod	conditionally,knocked
R6499	T10219	T10215	prt	out,knocked
R65	T226	T227	advmod	particularly,for
R650	T1554	T1553	amod	targeted,deletion
R6500	T10220	T10215	dobj	Bmp2,knocked
R6501	T10221	T10215	prep	in,knocked
R6502	T10222	T10223	det	the,background
R6503	T10223	T10221	pobj	background,in
R6504	T10224	T10223	nmod	Bmp7,background
R6505	T10225	T10224	punct	−,Bmp7
R6506	T10226	T10224	punct	/,Bmp7
R6507	T10227	T10224	punct	−,Bmp7
R6508	T10228	T10209	aux	do,show
R6509	T10229	T10209	neg	not,show
R651	T1556	T1553	prep	of,deletion
R6510	T10230	T10231	det	any,defect
R6511	T10231	T10209	dobj	defect,show
R6512	T10232	T10231	amod	obvious,defect
R6513	T10233	T10234	compound	digit,patterning
R6514	T10234	T10231	compound	patterning,defect
R6515	T10235	T10231	amod	other,defect
R6516	T10236	T10235	prep	than,other
R6517	T10237	T10238	det	the,phalanx
R6518	T10238	T10236	pobj	phalanx,than
R6519	T10239	T10238	amod	missing,phalanx
R652	T1557	T1556	pobj	Bmp7,of
R6520	T10240	T10238	amod	final,phalanx
R6521	T10241	T10238	prep	in,phalanx
R6522	T10242	T10241	pobj	digit,in
R6523	T10243	T10242	nummod	III,digit
R6524	T10244	T10189	punct	.,overlap
R6525	T10246	T10247	mark	Although,understood
R6526	T10247	T10250	advcl	understood,form
R6527	T10248	T10247	advmod	still,understood
R6528	T10249	T10247	advmod	poorly,understood
R6529	T10251	T10250	punct	", ",form
R653	T1558	T1555	aux	has,made
R6530	T10252	T10253	det	the,phalanges
R6531	T10253	T10250	nsubj	phalanges,form
R6532	T10254	T10253	amod	final,phalanges
R6533	T10255	T10250	prep	at,form
R6534	T10256	T10257	det	the,tip
R6535	T10257	T10255	pobj	tip,at
R6536	T10258	T10257	prep	of,tip
R6537	T10259	T10260	det	every,digit
R6538	T10260	T10258	pobj	digit,of
R6539	T10261	T10250	prep	through,form
R654	T1559	T1555	auxpass	been,made
R6540	T10262	T10263	det	a,process
R6541	T10263	T10261	pobj	process,through
R6542	T10264	T10263	amod	distinct,process
R6543	T10265	T10264	prep	from,distinct
R6544	T10266	T10267	det	the,phalanges
R6545	T10267	T10265	pobj	phalanges,from
R6546	T10268	T10269	advmod	more,proximal
R6547	T10269	T10267	amod	proximal,phalanges
R6548	T10270	T10271	punct	[,38
R6549	T10271	T10250	parataxis	38,form
R655	T1560	T1555	punct	", ",made
R6550	T10272	T10271	punct	],38
R6551	T10273	T10250	punct	.,form
R6552	T10275	T10276	poss	Our,data
R6553	T10276	T10277	nsubj	data,implicate
R6554	T10278	T10279	compound	BMP,signaling
R6555	T10279	T10277	dobj	signaling,implicate
R6556	T10280	T10277	prep	in,implicate
R6557	T10281	T10282	det	this,process
R6558	T10282	T10280	pobj	process,in
R6559	T10283	T10277	punct	.,implicate
R656	T1561	T1555	cc	and,made
R6560	T10285	T10286	prep	At,suggested
R6561	T10287	T10288	amod	later,stages
R6562	T10288	T10285	pobj	stages,At
R6563	T10289	T10286	punct	", ",suggested
R6564	T10290	T10291	compound	BMP,signaling
R6565	T10291	T10286	nsubjpass	signaling,suggested
R6566	T10292	T10286	aux	has,suggested
R6567	T10293	T10286	auxpass	been,suggested
R6568	T10294	T10295	aux	to,play
R6569	T10295	T10286	xcomp	play,suggested
R657	T1562	T1563	npadvmod	Bmp7,deficient
R6570	T10296	T10297	det	a,role
R6571	T10297	T10295	dobj	role,play
R6572	T10298	T10295	prep	in,play
R6573	T10299	T10298	pcomp	mediating,in
R6574	T10300	T10301	det	the,transfer
R6575	T10301	T10299	dobj	transfer,mediating
R6576	T10302	T10301	prep	of,transfer
R6577	T10303	T10304	amod	positional,information
R6578	T10304	T10302	pobj	information,of
R6579	T10305	T10301	prep	from,transfer
R658	T1563	T1565	amod	deficient,embryos
R6580	T10306	T10307	det	the,mesenchyme
R6581	T10307	T10305	pobj	mesenchyme,from
R6582	T10308	T10307	amod	interdigital,mesenchyme
R6583	T10309	T10310	aux	to,specify
R6584	T10310	T10295	advcl	specify,play
R6585	T10311	T10312	det	the,identity
R6586	T10312	T10310	dobj	identity,specify
R6587	T10313	T10312	prep	of,identity
R6588	T10314	T10315	det	the,digits
R6589	T10315	T10313	pobj	digits,of
R659	T1564	T1563	punct	-,deficient
R6590	T10316	T10315	amod	adjacent,digits
R6591	T10317	T10318	punct	[,15
R6592	T10318	T10286	parataxis	15,suggested
R6593	T10319	T10318	punct	],15
R6594	T10320	T10286	punct	.,suggested
R6595	T10322	T10323	nsubj	Application,results
R6596	T10324	T10322	prep	of,Application
R6597	T10325	T10326	det	the,noggin
R6598	T10326	T10324	pobj	noggin,of
R6599	T10327	T10326	compound	BMP,noggin
R66	T227	T223	prep	for,lethality
R660	T1565	T1566	nsubj	embryos,display
R6600	T10328	T10326	compound	antagonist,noggin
R6601	T10329	T10322	prep	to,Application
R6602	T10330	T10331	det	the,tissue
R6603	T10331	T10329	pobj	tissue,to
R6604	T10332	T10331	amod	interdigital,tissue
R6605	T10333	T10323	prep	at,results
R6606	T10334	T10335	det	the,time
R6607	T10335	T10333	pobj	time,at
R6608	T10336	T10335	prep	of,time
R6609	T10337	T10338	compound	digit,condensation
R661	T1566	T1555	conj	display,made
R6610	T10338	T10336	pobj	condensation,of
R6611	T10339	T10323	prep	in,results
R6612	T10340	T10339	pobj	loss,in
R6613	T10341	T10340	prep	of,loss
R6614	T10342	T10341	pobj	phalanges,of
R6615	T10343	T10340	cc	and,loss
R6616	T10344	T10345	det	a,pattern
R6617	T10345	T10340	conj	pattern,loss
R6618	T10346	T10345	amod	resultant,pattern
R6619	T10347	T10348	dep	that,interpreted
R662	T1567	T1568	compound	hindlimb,polydactyly
R6620	T10348	T10345	relcl	interpreted,pattern
R6621	T10349	T10348	aux	can,interpreted
R6622	T10350	T10348	auxpass	be,interpreted
R6623	T10351	T10348	prep	as,interpreted
R6624	T10352	T10353	det	an,transformation
R6625	T10353	T10351	pobj	transformation,as
R6626	T10354	T10353	amod	anterior,transformation
R6627	T10355	T10353	amod	homeotic,transformation
R6628	T10356	T10353	prep	in,transformation
R6629	T10357	T10358	compound	digit,identity
R663	T1568	T1566	dobj	polydactyly,display
R6630	T10358	T10356	pobj	identity,in
R6631	T10359	T10323	punct	.,results
R6632	T10361	T10362	advmod	However,result
R6633	T10363	T10362	punct	", ",result
R6634	T10364	T10362	csubj	increasing,result
R6635	T10365	T10366	compound	BMP,concentrations
R6636	T10366	T10364	dobj	concentrations,increasing
R6637	T10367	T10364	advmod	interdigitally,increasing
R6638	T10368	T10364	prep	with,increasing
R6639	T10369	T10370	amod	exogenous,protein
R664	T1569	T1568	prep	with,polydactyly
R6640	T10370	T10368	pobj	protein,with
R6641	T10371	T10362	aux	does,result
R6642	T10372	T10362	neg	not,result
R6643	T10373	T10362	prep	in,result
R6644	T10374	T10375	det	the,transformations
R6645	T10375	T10373	pobj	transformations,in
R6646	T10376	T10375	amod	reciprocal,transformations
R6647	T10377	T10375	amod	posterior,transformations
R6648	T10378	T10362	punct	", ",result
R6649	T10379	T10362	cc	and,result
R665	T1570	T1571	amod	incomplete,penetrance
R6650	T10380	T10381	det	the,experiments
R6651	T10381	T10383	nsubjpass	experiments,interpreted
R6652	T10382	T10381	compound	noggin,experiments
R6653	T10383	T10362	conj	interpreted,result
R6654	T10384	T10383	aux	can,interpreted
R6655	T10385	T10383	advmod	also,interpreted
R6656	T10386	T10383	auxpass	be,interpreted
R6657	T10387	T10383	advmod	simply,interpreted
R6658	T10388	T10383	prep	as,interpreted
R6659	T10389	T10390	det	a,block
R666	T1571	T1569	pobj	penetrance,with
R6660	T10390	T10388	pobj	block,as
R6661	T10391	T10390	prep	in,block
R6662	T10392	T10393	det	the,condensation
R6663	T10393	T10391	pobj	condensation,in
R6664	T10394	T10393	prep	of,condensation
R6665	T10395	T10396	det	the,primordia
R6666	T10396	T10394	pobj	primordia,of
R6667	T10397	T10396	prep	of,primordia
R6668	T10398	T10399	amod	distal,phalanges
R6669	T10399	T10397	pobj	phalanges,of
R667	T1572	T1568	cc	but,polydactyly
R6670	T10400	T10401	advmod	when,abolished
R6671	T10401	T10383	advcl	abolished,interpreted
R6672	T10402	T10403	compound	BMP,activity
R6673	T10403	T10401	nsubjpass	activity,abolished
R6674	T10404	T10401	auxpass	is,abolished
R6675	T10405	T10401	advmod	completely,abolished
R6676	T10406	T10407	punct	[,38
R6677	T10407	T10383	parataxis	38,interpreted
R6678	T10408	T10407	punct	],38
R6679	T10409	T10383	punct	.,interpreted
R668	T1573	T1574	advmod	otherwise,normal
R6680	T10411	T10412	csubj	Decreasing,provides
R6681	T10413	T10414	det	the,level
R6682	T10414	T10411	dobj	level,Decreasing
R6683	T10415	T10414	prep	of,level
R6684	T10416	T10417	amod	interdigital,signaling
R6685	T10417	T10415	pobj	signaling,of
R6686	T10418	T10417	compound	BMP,signaling
R6687	T10419	T10411	punct	", ",Decreasing
R6688	T10420	T10411	prep	without,Decreasing
R6689	T10421	T10420	pcomp	abolishing,without
R669	T1574	T1576	amod	normal,limbs
R6690	T10422	T10421	dobj	it,abolishing
R6691	T10423	T10412	punct	", ",provides
R6692	T10424	T10425	det	an,opportunity
R6693	T10425	T10412	dobj	opportunity,provides
R6694	T10426	T10425	prep	for,opportunity
R6695	T10427	T10426	pcomp	differentiating,for
R6696	T10428	T10427	prep	between,differentiating
R6697	T10429	T10430	det	these,models
R6698	T10430	T10428	pobj	models,between
R6699	T10431	T10412	punct	.,provides
R67	T228	T227	pobj	Bmp2,for
R670	T1575	T1574	advmod	phenotypically,normal
R6700	T10433	T10434	mark	If,required
R6701	T10434	T10439	advcl	required,have
R6702	T10435	T10436	compound	BMP,signaling
R6703	T10436	T10434	nsubjpass	signaling,required
R6704	T10437	T10434	auxpass	were,required
R6705	T10438	T10434	advmod	only,required
R6706	T10440	T10434	prep	as,required
R6707	T10441	T10442	det	a,factor
R6708	T10442	T10440	pobj	factor,as
R6709	T10443	T10442	amod	permissive,factor
R671	T1576	T1568	conj	limbs,polydactyly
R6710	T10444	T10442	prep	for,factor
R6711	T10445	T10444	pobj	chondrogenesis,for
R6712	T10446	T10439	punct	", ",have
R6713	T10447	T10448	amod	partial,reduction
R6714	T10448	T10439	nsubj	reduction,have
R6715	T10449	T10448	prep	in,reduction
R6716	T10450	T10451	det	the,level
R6717	T10451	T10449	pobj	level,in
R6718	T10452	T10451	prep	of,level
R6719	T10453	T10452	pobj	signaling,of
R672	T1577	T1578	punct	[,13
R6720	T10454	T10439	aux	might,have
R6721	T10455	T10456	det	no,effect
R6722	T10456	T10439	dobj	effect,have
R6723	T10457	T10439	punct	.,have
R6724	T10459	T10460	advmod	However,cause
R6725	T10461	T10460	punct	", ",cause
R6726	T10462	T10463	mark	if,were
R6727	T10463	T10460	advcl	were,cause
R6728	T10464	T10463	nsubj	levels,were
R6729	T10465	T10464	prep	of,levels
R673	T1578	T1566	parataxis	13,display
R6730	T10466	T10467	compound	BMP,signaling
R6731	T10467	T10465	pobj	signaling,of
R6732	T10468	T10463	acomp	instructive,were
R6733	T10469	T10463	prep	in,were
R6734	T10470	T10469	pobj	terms,in
R6735	T10471	T10470	prep	of,terms
R6736	T10472	T10473	compound	digit,identities
R6737	T10473	T10471	pobj	identities,of
R6738	T10474	T10460	punct	", ",cause
R6739	T10475	T10460	advmod	then,cause
R674	T1579	T1578	nummod	12,13
R6740	T10476	T10477	det	a,decrease
R6741	T10477	T10460	nsubj	decrease,cause
R6742	T10478	T10477	amod	substantial,decrease
R6743	T10479	T10477	prep	in,decrease
R6744	T10480	T10481	det	the,amount
R6745	T10481	T10479	pobj	amount,in
R6746	T10482	T10481	prep	of,amount
R6747	T10483	T10484	compound	BMP,ligand
R6748	T10484	T10482	pobj	ligand,of
R6749	T10485	T10484	amod	present,ligand
R675	T1580	T1578	punct	",",13
R6750	T10486	T10460	aux	should,cause
R6751	T10487	T10460	dobj	transformations,cause
R6752	T10488	T10487	prep	in,transformations
R6753	T10489	T10490	compound	digit,identity
R6754	T10490	T10488	pobj	identity,in
R6755	T10491	T10460	punct	.,cause
R6756	T10493	T10494	nsubj	This,is
R6757	T10495	T10494	neg	not,is
R6758	T10496	T10497	dep	what,observed
R6759	T10497	T10494	ccomp	observed,is
R676	T1581	T1578	punct	],13
R6760	T10498	T10497	auxpass	is,observed
R6761	T10499	T10494	punct	", ",is
R6762	T10500	T10501	preconj	either,in
R6763	T10501	T10494	prep	in,is
R6764	T10502	T10501	pobj	limbs,in
R6765	T10503	T10502	amod	deficient,limbs
R6766	T10504	T10503	prep	in,deficient
R6767	T10505	T10506	preconj	both,BMP2
R6768	T10506	T10504	pobj	BMP2,in
R6769	T10507	T10506	cc	and,BMP2
R677	T1582	T1566	punct	.,display
R6770	T10508	T10506	conj	BMP4,BMP2
R6771	T10509	T10501	cc	or,in
R6772	T10510	T10501	conj	in,in
R6773	T10511	T10510	pobj	those,in
R6774	T10512	T10511	amod	deficient,those
R6775	T10513	T10512	prep	in,deficient
R6776	T10514	T10513	pobj	BMP2,in
R6777	T10515	T10514	cc	and,BMP2
R6778	T10516	T10514	conj	BMP7,BMP2
R6779	T10517	T10494	punct	.,is
R678	T1584	T1585	advmod	Nonetheless,show
R6780	T10519	T10520	nsubjpass	It,noted
R6781	T10521	T10520	aux	should,noted
R6782	T10522	T10520	auxpass	be,noted
R6783	T10523	T10524	mark	that,form
R6784	T10524	T10520	ccomp	form,noted
R6785	T10525	T10526	det	the,domains
R6786	T10526	T10524	nsubj	domains,form
R6787	T10527	T10526	amod	interdigital,domains
R6788	T10528	T10526	compound	expression,domains
R6789	T10529	T10530	amod	several,days
R679	T1586	T1585	punct	", ",show
R6790	T10530	T10531	npadvmod	days,is
R6791	T10531	T10524	advcl	is,form
R6792	T10532	T10531	mark	after,is
R6793	T10533	T10531	nsubj	recombination,is
R6794	T10534	T10533	prep	of,recombination
R6795	T10535	T10536	det	the,alleles
R6796	T10536	T10534	pobj	alleles,of
R6797	T10537	T10536	amod	floxed,alleles
R6798	T10538	T10536	compound	BMP,alleles
R6799	T10539	T10531	acomp	complete,is
R68	T229	T228	cc	and,Bmp2
R680	T1587	T1588	compound	Bmp7,knockout
R6800	T10540	T10520	punct	.,noted
R6801	T10542	T10543	advmod	Moreover,is
R6802	T10544	T10543	punct	", ",is
R6803	T10545	T10543	expl	there,is
R6804	T10546	T10547	det	no,upregulation
R6805	T10547	T10543	attr	upregulation,is
R6806	T10548	T10547	amod	detectable,upregulation
R6807	T10549	T10547	amod	compensatory,upregulation
R6808	T10550	T10547	prep	of,upregulation
R6809	T10551	T10550	pobj	BMP7,of
R681	T1588	T1589	compound	knockout,mice
R6810	T10552	T10543	prep	in,is
R6811	T10553	T10554	det	the,mesenchyme
R6812	T10554	T10552	pobj	mesenchyme,in
R6813	T10555	T10554	amod	interdigital,mesenchyme
R6814	T10556	T10554	prep	of,mesenchyme
R6815	T10557	T10558	det	the,limbs
R6816	T10558	T10556	pobj	limbs,of
R6817	T10559	T10560	nmod	BMP2,BMP4
R6818	T10560	T10562	npadvmod	BMP4,deficient
R6819	T10561	T10560	punct	", ",BMP4
R682	T1589	T1585	nsubj	mice,show
R6820	T10562	T10558	amod	deficient,limbs
R6821	T10563	T10562	punct	–,deficient
R6822	T10564	T10565	punct	(,compare
R6823	T10565	T10558	parataxis	compare,limbs
R6824	T10566	T10567	compound	Figure,4G
R6825	T10567	T10565	dobj	4G,compare
R6826	T10568	T10567	cc	and,4G
R6827	T10569	T10570	compound	Figure,4H
R6828	T10570	T10567	conj	4H,4G
R6829	T10571	T10565	punct	),compare
R683	T1590	T1585	aux	do,show
R6830	T10572	T10543	punct	.,is
R6831	T10574	T10575	advmod	Thus,conclude
R6832	T10576	T10575	punct	", ",conclude
R6833	T10577	T10575	nsubj	we,conclude
R6834	T10578	T10579	mark	that,is
R6835	T10579	T10575	ccomp	is,conclude
R6836	T10580	T10581	compound	BMP,signaling
R6837	T10581	T10579	nsubj	signaling,is
R6838	T10582	T10579	neg	not,is
R6839	T10583	T10579	acomp	likely,is
R684	T1591	T1585	neg	not,show
R6840	T10584	T10585	aux	to,be
R6841	T10585	T10583	xcomp	be,likely
R6842	T10586	T10585	acomp	responsible,be
R6843	T10587	T10585	prep	in,be
R6844	T10588	T10589	det	a,fashion
R6845	T10589	T10587	pobj	fashion,in
R6846	T10590	T10589	amod	quantitative,fashion
R6847	T10591	T10585	prep	for,be
R6848	T10592	T10593	det	the,role
R6849	T10593	T10591	pobj	role,for
R685	T1592	T1593	det	any,defect
R6850	T10594	T10593	amod	instructive,role
R6851	T10595	T10593	prep	of,role
R6852	T10596	T10597	amod	interdigital,mesenchyme
R6853	T10597	T10595	pobj	mesenchyme,of
R6854	T10598	T10593	prep	in,role
R6855	T10599	T10598	pcomp	establishing,in
R6856	T10600	T10601	compound	digit,identities
R6857	T10601	T10599	dobj	identities,establishing
R6858	T10602	T10575	punct	.,conclude
R6859	T10604	T10605	advmod	However,is
R686	T1593	T1585	dobj	defect,show
R6860	T10606	T10605	punct	", ",is
R6861	T10607	T10605	prep	in,is
R6862	T10608	T10609	det	the,forelimbs
R6863	T10609	T10607	pobj	forelimbs,in
R6864	T10610	T10611	nmod	Bmp2C,C
R6865	T10611	T10609	nmod	C,forelimbs
R6866	T10612	T10611	punct	/,C
R6867	T10613	T10611	punct	;,C
R6868	T10614	T10615	compound	Bmp4C,C
R6869	T10615	T10611	appos	C,C
R687	T1594	T1593	prep	in,defect
R6870	T10616	T10615	punct	/,C
R6871	T10617	T10611	punct	;,C
R6872	T10618	T10619	compound	Prx1,cre
R6873	T10619	T10611	appos	cre,C
R6874	T10620	T10619	punct	:,cre
R6875	T10621	T10619	punct	:,cre
R6876	T10622	T10605	punct	", ",is
R6877	T10623	T10605	expl	there,is
R6878	T10624	T10625	det	a,loss
R6879	T10625	T10605	attr	loss,is
R688	T1595	T1596	compound	limb,polarity
R6880	T10626	T10625	amod	complete,loss
R6881	T10627	T10625	prep	of,loss
R6882	T10628	T10629	amod	posterior,digit
R6883	T10629	T10630	compound	digit,condensations
R6884	T10630	T10627	pobj	condensations,of
R6885	T10631	T10630	compound	ray,condensations
R6886	T10632	T10605	punct	", ",is
R6887	T10633	T10634	advmod	presumably,falls
R6888	T10634	T10605	advcl	falls,is
R6889	T10635	T10634	mark	because,falls
R689	T1596	T1594	pobj	polarity,in
R6890	T10636	T10637	det	the,level
R6891	T10637	T10634	nsubj	level,falls
R6892	T10638	T10637	amod	total,level
R6893	T10639	T10637	prep	of,level
R6894	T10640	T10641	compound	BMP,signaling
R6895	T10641	T10639	pobj	signaling,of
R6896	T10642	T10634	prep	below,falls
R6897	T10643	T10644	det	the,threshold
R6898	T10644	T10642	pobj	threshold,below
R6899	T10645	T10644	prep	for,threshold
R69	T230	T228	conj	Bmp4,Bmp2
R690	T1597	T1585	punct	.,show
R6900	T10646	T10645	pobj	initiation,for
R6901	T10647	T10646	prep	of,initiation
R6902	T10648	T10647	pobj	condensation,of
R6903	T10649	T10634	punct	", ",falls
R6904	T10650	T10651	mark	as,seen
R6905	T10651	T10634	advcl	seen,falls
R6906	T10652	T10651	advmod	previously,seen
R6907	T10653	T10651	prep	in,seen
R6908	T10654	T10653	pobj	cases,in
R6909	T10655	T10656	advmod	where,used
R691	T1599	T1600	advmod	However,remains
R6910	T10656	T10654	relcl	used,cases
R6911	T10657	T10658	compound	BMP,antagonists
R6912	T10658	T10656	nsubjpass	antagonists,used
R6913	T10659	T10656	aux	have,used
R6914	T10660	T10656	auxpass	been,used
R6915	T10661	T10662	punct	[,28
R6916	T10662	T10605	parataxis	28,is
R6917	T10663	T10662	nummod	27,28
R6918	T10664	T10662	punct	",",28
R6919	T10665	T10662	punct	],28
R692	T1601	T1600	punct	", ",remains
R6920	T10666	T10605	punct	.,is
R6921	T10668	T10669	nsubj	It,is
R6922	T10670	T10669	acomp	worth,is
R6923	T10671	T10670	advcl	noting,worth
R6924	T10672	T10673	mark	that,are
R6925	T10673	T10669	ccomp	are,is
R6926	T10674	T10675	det	the,defects
R6927	T10675	T10673	nsubj	defects,are
R6928	T10676	T10675	prep	in,defects
R6929	T10677	T10676	pobj	chondrogenesis,in
R693	T1602	T1603	det	a,function
R6930	T10678	T10675	acl	seen,defects
R6931	T10679	T10678	prep	in,seen
R6932	T10680	T10681	det	the,limbs
R6933	T10681	T10679	pobj	limbs,in
R6934	T10682	T10683	nmod	BMP2,BMP4
R6935	T10683	T10685	npadvmod	BMP4,deficient
R6936	T10684	T10683	punct	", ",BMP4
R6937	T10685	T10681	amod	deficient,limbs
R6938	T10686	T10685	punct	–,deficient
R6939	T10687	T10688	advmod	much,more
R694	T1603	T1600	nsubj	function,remains
R6940	T10688	T10689	advmod	more,severe
R6941	T10689	T10673	acomp	severe,are
R6942	T10690	T10673	advmod	proximally,are
R6943	T10691	T10673	prep	than,are
R6944	T10692	T10691	pcomp	distally,than
R6945	T10693	T10669	punct	.,is
R6946	T10695	T10696	nsubj	This,is
R6947	T10697	T10698	advmod	particularly,significant
R6948	T10698	T10696	acomp	significant,is
R6949	T10699	T10700	mark	because,condense
R695	T1604	T1603	amod	redundant,function
R6950	T10700	T10696	advcl	condense,is
R6951	T10701	T10702	amod	proximal,elements
R6952	T10702	T10700	nsubj	elements,condense
R6953	T10703	T10700	cc	and,condense
R6954	T10704	T10700	conj	differentiate,condense
R6955	T10705	T10704	prep	before,differentiate
R6956	T10706	T10707	amod	distal,ones
R6957	T10707	T10705	pobj	ones,before
R6958	T10708	T10696	punct	.,is
R6959	T10710	T10711	advmod	Thus,is
R696	T1605	T1603	prep	in,function
R6960	T10712	T10711	punct	", ",is
R6961	T10713	T10714	mark	if,recombined
R6962	T10714	T10711	advcl	recombined,is
R6963	T10715	T10716	det	the,alleles
R6964	T10716	T10714	nsubjpass	alleles,recombined
R6965	T10717	T10716	amod	conditional,alleles
R6966	T10718	T10714	auxpass	were,recombined
R6967	T10719	T10714	advmod	partially,recombined
R6968	T10720	T10714	prep	at,recombined
R6969	T10721	T10722	amod	early,stages
R697	T1606	T1607	amod	anterior,posterior
R6970	T10722	T10720	pobj	stages,at
R6971	T10723	T10714	cc	and,recombined
R6972	T10724	T10725	advmod	only,completely
R6973	T10725	T10726	advmod	completely,recombined
R6974	T10726	T10714	conj	recombined,recombined
R6975	T10727	T10726	prep	at,recombined
R6976	T10728	T10729	amod	later,stages
R6977	T10729	T10727	pobj	stages,at
R6978	T10730	T10711	punct	", ",is
R6979	T10731	T10711	nsubj	it,is
R698	T1607	T1609	amod	posterior,patterning
R6980	T10732	T10733	det	the,elements
R6981	T10733	T10711	attr	elements,is
R6982	T10734	T10733	amod	distal,elements
R6983	T10735	T10733	punct	", ",elements
R6984	T10736	T10733	acl	formed,elements
R6985	T10737	T10738	mark	after,taken
R6986	T10738	T10736	advcl	taken,formed
R6987	T10739	T10740	amod	greater,recombination
R6988	T10740	T10738	nsubj	recombination,taken
R6989	T10741	T10738	aux	has,taken
R699	T1608	T1607	punct	-,posterior
R6990	T10742	T10738	dobj	place,taken
R6991	T10743	T10711	punct	", ",is
R6992	T10744	T10745	dep	that,affected
R6993	T10745	T10711	ccomp	affected,is
R6994	T10746	T10745	aux	should,affected
R6995	T10747	T10745	auxpass	be,affected
R6996	T10748	T10749	advmod	more,severely
R6997	T10749	T10745	advmod	severely,affected
R6998	T10750	T10711	punct	.,is
R6999	T10752	T10753	mark	Since,is
R7	T161	T159	conj	BMP4,BMP2
R70	T231	T221	punct	", ",with
R700	T1609	T1605	pobj	patterning,in
R7000	T10753	T10755	advcl	is,lie
R7001	T10754	T10753	nsubj	it,is
R7002	T10756	T10757	det	the,elements
R7003	T10757	T10753	attr	elements,is
R7004	T10758	T10757	amod	proximal,elements
R7005	T10759	T10760	dep	which,affected
R7006	T10760	T10753	ccomp	affected,is
R7007	T10761	T10760	auxpass	are,affected
R7008	T10762	T10763	advmod	more,severely
R7009	T10763	T10760	advmod	severely,affected
R701	T1610	T1603	prep	with,function
R7010	T10764	T10755	punct	", ",lie
R7011	T10765	T10766	det	the,explanation
R7012	T10766	T10755	nsubj	explanation,lie
R7013	T10767	T10766	prep	for,explanation
R7014	T10768	T10769	det	the,difference
R7015	T10769	T10767	pobj	difference,for
R7016	T10770	T10769	prep	in,difference
R7017	T10771	T10770	pobj	severity,in
R7018	T10772	T10755	aux	must,lie
R7019	T10773	T10755	advmod	elsewhere,lie
R702	T1611	T1610	pobj	BMP2,with
R7020	T10774	T10755	punct	.,lie
R7021	T10776	T10777	nsubjpass	This,explained
R7022	T10778	T10777	auxpass	is,explained
R7023	T10779	T10780	advmod	most,likely
R7024	T10780	T10777	advmod	likely,explained
R7025	T10781	T10777	prep	by,explained
R7026	T10782	T10781	pobj	compensation,by
R7027	T10783	T10782	prep	in,compensation
R7028	T10784	T10785	det	the,bud
R7029	T10785	T10783	pobj	bud,in
R703	T1612	T1613	det	a,possibility
R7030	T10786	T10785	amod	distal,bud
R7031	T10787	T10785	compound	limb,bud
R7032	T10788	T10782	prep	by,compensation
R7033	T10789	T10790	nmod	BMP2,BMP4
R7034	T10790	T10788	pobj	BMP4,by
R7035	T10791	T10790	punct	", ",BMP4
R7036	T10792	T10790	punct	", ",BMP4
R7037	T10793	T10790	cc	and,BMP4
R7038	T10794	T10790	conj	BMP7,BMP4
R7039	T10795	T10794	acl	produced,BMP7
R704	T1613	T1600	attr	possibility,remains
R7040	T10796	T10795	prep	in,produced
R7041	T10797	T10798	det	the,AER
R7042	T10798	T10796	pobj	AER,in
R7043	T10799	T10777	punct	.,explained
R7044	T10801	T10802	nsubj	Compensation,explains
R7045	T10803	T10801	prep	by,Compensation
R7046	T10804	T10803	pobj	BMPs,by
R7047	T10805	T10804	prep	in,BMPs
R7048	T10806	T10807	det	the,AER
R7049	T10807	T10805	pobj	AER,in
R705	T1614	T1600	punct	.,remains
R7050	T10808	T10802	advmod	also,explains
R7051	T10809	T10802	advmod	likely,explains
R7052	T10810	T10811	det	a,difference
R7053	T10811	T10802	dobj	difference,explains
R7054	T10812	T10811	acl	seen,difference
R7055	T10813	T10812	prep	between,seen
R7056	T10814	T10815	det	the,mice
R7057	T10815	T10813	pobj	mice,between
R7058	T10816	T10817	nmod	Bmp4C,C
R7059	T10817	T10815	nmod	C,mice
R706	T1616	T1617	prep	In,expressed
R7060	T10818	T10817	punct	/,C
R7061	T10819	T10817	punct	;,C
R7062	T10820	T10821	compound	Prx1,cre
R7063	T10821	T10817	appos	cre,C
R7064	T10822	T10821	punct	:,cre
R7065	T10823	T10821	punct	:,cre
R7066	T10824	T10812	prep	in,seen
R7067	T10825	T10826	poss	our,experiments
R7068	T10826	T10824	pobj	experiments,in
R7069	T10827	T10826	cc	and,experiments
R707	T1618	T1616	pobj	addition,In
R7070	T10828	T10829	det	a,analysis
R7071	T10829	T10826	conj	analysis,experiments
R7072	T10830	T10831	advmod	recently,published
R7073	T10831	T10829	amod	published,analysis
R7074	T10832	T10833	punct	[,16
R7075	T10833	T10829	parataxis	16,analysis
R7076	T10834	T10833	punct	],16
R7077	T10835	T10829	punct	", ",analysis
R7078	T10836	T10837	dep	which,used
R7079	T10837	T10829	relcl	used,analysis
R708	T1619	T1618	prep	to,addition
R7080	T10838	T10839	det	the,allele
R7081	T10839	T10837	dobj	allele,used
R7082	T10840	T10839	amod	identical,allele
R7083	T10841	T10839	nmod	Bmp4,allele
R7084	T10842	T10839	amod	conditional,allele
R7085	T10843	T10839	cc	and,allele
R7086	T10844	T10845	compound	Prx1,cre
R7087	T10845	T10848	compound	cre,driver
R7088	T10846	T10845	punct	:,cre
R7089	T10847	T10845	punct	:,cre
R709	T1620	T1619	pobj	BMP2,to
R7090	T10848	T10839	conj	driver,allele
R7091	T10849	T10802	punct	.,explains
R7092	T10851	T10852	det	The,report
R7093	T10852	T10854	nsubj	report,described
R7094	T10853	T10852	amod	earlier,report
R7095	T10855	T10854	punct	", ",described
R7096	T10856	T10854	prep	like,described
R7097	T10857	T10856	pobj	ours,like
R7098	T10858	T10854	punct	", ",described
R7099	T10859	T10860	amod	preaxial,polydactyly
R71	T232	T221	cc	and,with
R710	T1621	T1620	cc	and,BMP2
R7100	T10860	T10854	dobj	polydactyly,described
R7101	T10861	T10859	cc	and,preaxial
R7102	T10862	T10859	conj	postaxial,preaxial
R7103	T10863	T10854	punct	.,described
R7104	T10865	T10866	advmod	However,observed
R7105	T10867	T10866	punct	", ",observed
R7106	T10868	T10866	nsubj	they,observed
R7107	T10869	T10870	det	these,phenotypes
R7108	T10870	T10866	dobj	phenotypes,observed
R7109	T10871	T10866	prep	with,observed
R711	T1622	T1620	conj	BMP7,BMP2
R7110	T10872	T10873	amod	less,variability
R7111	T10873	T10871	pobj	variability,with
R7112	T10874	T10866	cc	and,observed
R7113	T10875	T10866	punct	", ",observed
R7114	T10876	T10877	advmod	moreover,described
R7115	T10877	T10866	conj	described,observed
R7116	T10878	T10877	punct	", ",described
R7117	T10879	T10880	amod	multiple,digits
R7118	T10880	T10877	dobj	digits,described
R7119	T10881	T10880	amod	preaxial,digits
R712	T1623	T1617	punct	", ",expressed
R7120	T10882	T10881	cc	and,preaxial
R7121	T10883	T10881	conj	postaxial,preaxial
R7122	T10884	T10880	amod	ectopic,digits
R7123	T10885	T10866	punct	", ",observed
R7124	T10886	T10887	mark	while,saw
R7125	T10887	T10866	advcl	saw,observed
R7126	T10888	T10887	nsubj	we,saw
R7127	T10889	T10887	punct	", ",saw
R7128	T10890	T10891	advmod	at,most
R7129	T10891	T10887	advmod	most,saw
R713	T1624	T1625	det	a,member
R7130	T10892	T10887	punct	", ",saw
R7131	T10893	T10894	det	a,digit
R7132	T10894	T10887	dobj	digit,saw
R7133	T10895	T10894	amod	single,digit
R7134	T10896	T10894	amod	preaxial,digit
R7135	T10897	T10896	cc	and,preaxial
R7136	T10898	T10896	conj	postaxial,preaxial
R7137	T10899	T10866	punct	.,observed
R7138	T10901	T10902	det	The,difference
R7139	T10902	T10903	nsubj	difference,is
R714	T1625	T1617	nsubjpass	member,expressed
R7140	T10904	T10905	mark	that,had
R7141	T10905	T10903	ccomp	had,is
R7142	T10906	T10907	det	the,mice
R7143	T10907	T10905	nsubj	mice,had
R7144	T10908	T10907	acl	analyzed,mice
R7145	T10909	T10908	prep	in,analyzed
R7146	T10910	T10911	det	the,report
R7147	T10911	T10909	pobj	report,in
R7148	T10912	T10911	amod	previous,report
R7149	T10913	T10914	nummod	one,allele
R715	T1626	T1625	amod	third,member
R7150	T10914	T10905	dobj	allele,had
R7151	T10915	T10914	amod	null,allele
R7152	T10916	T10914	cc	and,allele
R7153	T10917	T10918	nummod	one,allele
R7154	T10918	T10914	conj	allele,allele
R7155	T10919	T10918	amod	conditional,allele
R7156	T10920	T10903	punct	", ",is
R7157	T10921	T10922	mark	while,had
R7158	T10922	T10903	advcl	had,is
R7159	T10923	T10922	nsubj	ours,had
R716	T1627	T1625	prep	of,member
R7160	T10924	T10925	nummod	two,alleles
R7161	T10925	T10922	dobj	alleles,had
R7162	T10926	T10925	amod	conditional,alleles
R7163	T10927	T10903	punct	.,is
R7164	T10929	T10930	poss	Their,mice
R7165	T10930	T10931	nsubj	mice,lost
R7166	T10932	T10931	punct	", ",lost
R7167	T10933	T10931	advmod	therefore,lost
R7168	T10934	T10931	punct	", ",lost
R7169	T10935	T10936	det	a,dose
R717	T1628	T1629	det	this,family
R7170	T10936	T10931	dobj	dose,lost
R7171	T10937	T10936	amod	half,dose
R7172	T10938	T10936	punct	-,dose
R7173	T10939	T10936	prep	of,dose
R7174	T10940	T10939	pobj	BMP4,of
R7175	T10941	T10931	prep	from,lost
R7176	T10942	T10943	det	the,AER
R7177	T10943	T10941	pobj	AER,from
R7178	T10944	T10931	prep	in,lost
R7179	T10945	T10944	pobj	addition,in
R718	T1629	T1627	pobj	family,of
R7180	T10946	T10945	prep	to,addition
R7181	T10947	T10948	amod	complete,loss
R7182	T10948	T10946	pobj	loss,to
R7183	T10949	T10948	prep	in,loss
R7184	T10950	T10951	det	the,mesenchyme
R7185	T10951	T10949	pobj	mesenchyme,in
R7186	T10952	T10931	punct	", ",lost
R7187	T10953	T10954	mark	while,recombined
R7188	T10954	T10931	advcl	recombined,lost
R7189	T10955	T10956	poss	our,alleles
R719	T1630	T1631	dep	that,related
R7190	T10956	T10954	nsubjpass	alleles,recombined
R7191	T10957	T10956	amod	conditional,alleles
R7192	T10958	T10954	auxpass	were,recombined
R7193	T10959	T10954	advmod	only,recombined
R7194	T10960	T10954	prep	in,recombined
R7195	T10961	T10962	det	the,mesenchyme
R7196	T10962	T10960	pobj	mesenchyme,in
R7197	T10963	T10954	prep	by,recombined
R7198	T10964	T10965	det	the,driver
R7199	T10965	T10963	pobj	driver,by
R72	T233	T221	conj	with,with
R720	T1631	T1625	relcl	related,member
R7200	T10966	T10967	compound	Prx1,cre
R7201	T10967	T10965	compound	cre,driver
R7202	T10968	T10967	punct	:,cre
R7203	T10969	T10967	punct	:,cre
R7204	T10970	T10931	punct	.,lost
R7205	T10972	T10973	nsubj	It,is
R7206	T10973	T10974	ccomp	is,favor
R7207	T10975	T10973	advmod	also,is
R7208	T10976	T10973	acomp	possible,is
R7209	T10977	T10978	mark	that,relates
R721	T1632	T1631	auxpass	is,related
R7210	T10978	T10973	ccomp	relates,is
R7211	T10979	T10980	det	the,difference
R7212	T10980	T10978	nsubj	difference,relates
R7213	T10981	T10980	prep	in,difference
R7214	T10982	T10981	pobj	severity,in
R7215	T10983	T10978	prep	to,relates
R7216	T10984	T10985	det	the,time
R7217	T10985	T10983	pobj	time,to
R7218	T10986	T10985	acl	required,time
R7219	T10987	T10988	aux	to,recombine
R722	T1633	T1631	advmod	closely,related
R7220	T10988	T10986	advcl	recombine,required
R7221	T10989	T10988	dobj	one,recombine
R7222	T10990	T10991	dep	as,opposed
R7223	T10991	T10989	cc	opposed,one
R7224	T10992	T10991	dep	to,opposed
R7225	T10993	T10994	nummod	two,alleles
R7226	T10994	T10989	conj	alleles,one
R7227	T10995	T10994	amod	floxed,alleles
R7228	T10996	T10974	punct	;,favor
R7229	T10997	T10974	advmod	however,favor
R723	T1634	T1631	prep	to,related
R7230	T10998	T10974	punct	", ",favor
R7231	T10999	T10974	nsubj	we,favor
R7232	T11000	T10974	aux	do,favor
R7233	T11001	T10974	neg	not,favor
R7234	T11002	T11003	det	this,explanation
R7235	T11003	T10974	dobj	explanation,favor
R7236	T11004	T11005	mark	as,lost
R7237	T11005	T10974	advcl	lost,favor
R7238	T11006	T11007	det	all,expression
R7239	T11007	T11005	nsubjpass	expression,lost
R724	T1635	T1634	pobj	BMP2,to
R7240	T11008	T11007	amod	detectable,expression
R7241	T11009	T11007	compound	BMP4,expression
R7242	T11010	T11005	auxpass	is,lost
R7243	T11011	T11012	amod	prior,to
R7244	T11012	T11005	prep	to,lost
R7245	T11013	T11014	det	any,differences
R7246	T11014	T11012	pobj	differences,to
R7247	T11015	T11014	amod	morphological,differences
R7248	T11016	T11014	prep	between,differences
R7249	T11017	T11018	det	the,buds
R725	T1636	T1625	punct	", ",member
R7250	T11018	T11016	pobj	buds,between
R7251	T11019	T11018	amod	mutant,buds
R7252	T11020	T11019	cc	and,mutant
R7253	T11021	T11022	amod	wild,type
R7254	T11022	T11019	conj	type,mutant
R7255	T11023	T11022	punct	-,type
R7256	T11024	T11018	compound	limb,buds
R7257	T11025	T10974	punct	.,favor
R7258	T11027	T11028	advmod	Finally,contribute
R7259	T11029	T11028	punct	", ",contribute
R726	T1637	T1625	appos	BMP4,member
R7260	T11030	T11028	nsubj	differences,contribute
R7261	T11031	T11030	prep	in,differences
R7262	T11032	T11033	amod	genetic,background
R7263	T11033	T11031	pobj	background,in
R7264	T11034	T11028	aux	could,contribute
R7265	T11035	T11028	prep	to,contribute
R7266	T11036	T11037	det	the,differences
R7267	T11037	T11035	pobj	differences,to
R7268	T11038	T11039	mark	as,outbred
R7269	T11039	T11028	advcl	outbred,contribute
R727	T1638	T1617	punct	", ",expressed
R7270	T11040	T11041	det	the,line
R7271	T11041	T11039	nsubjpass	line,outbred
R7272	T11042	T11043	nmod	Prx1,cre
R7273	T11043	T11041	compound	cre,line
R7274	T11044	T11043	punct	:,cre
R7275	T11045	T11043	punct	:,cre
R7276	T11046	T11039	auxpass	is,outbred
R7277	T11047	T11028	punct	.,contribute
R7278	T11049	T11050	det	A,aspect
R7279	T11050	T11052	nsubj	aspect,is
R728	T1639	T1617	auxpass	is,expressed
R7280	T11051	T11050	amod	final,aspect
R7281	T11053	T11050	prep	of,aspect
R7282	T11054	T11053	pobj	patterning,of
R7283	T11055	T11056	advmod	previously,associated
R7284	T11056	T11050	acl	associated,aspect
R7285	T11057	T11056	prep	with,associated
R7286	T11058	T11059	compound	BMP,signaling
R7287	T11059	T11057	pobj	signaling,with
R7288	T11060	T11061	amod	interdigital,apoptosis
R7289	T11061	T11052	attr	apoptosis,is
R729	T1640	T1617	advmod	also,expressed
R7290	T11062	T11052	punct	.,is
R7291	T11064	T11065	advcl	Consistent,see
R7292	T11066	T11064	prep	with,Consistent
R7293	T11067	T11066	pobj	this,with
R7294	T11068	T11065	punct	", ",see
R7295	T11069	T11065	nsubj	we,see
R7296	T11070	T11071	det	some,syndactyly
R7297	T11071	T11065	dobj	syndactyly,see
R7298	T11072	T11065	prep	in,see
R7299	T11073	T11074	det	the,limbs
R73	T234	T235	amod	functional,redundancy
R730	T1641	T1617	prep	in,expressed
R7300	T11074	T11072	pobj	limbs,in
R7301	T11075	T11076	npadvmod	BMP2,deficient
R7302	T11076	T11074	amod	deficient,limbs
R7303	T11077	T11076	punct	-,deficient
R7304	T11078	T11065	punct	.,see
R7305	T11080	T11081	det	The,fact
R7306	T11081	T11082	nsubj	fact,implies
R7307	T11083	T11084	mark	that,is
R7308	T11084	T11081	acl	is,fact
R7309	T11085	T11084	nsubj	this,is
R731	T1642	T1643	det	the,bud
R7310	T11086	T11084	acomp	variable,is
R7311	T11087	T11086	punct	", ",variable
R7312	T11088	T11086	cc	and,variable
R7313	T11089	T11086	conj	limited,variable
R7314	T11090	T11089	prep	to,limited
R7315	T11091	T11092	det	the,interdigit
R7316	T11092	T11090	pobj	interdigit,to
R7317	T11093	T11094	nummod	3,4
R7318	T11094	T11092	nummod	4,interdigit
R7319	T11095	T11094	punct	/,4
R732	T1643	T1641	pobj	bud,in
R7320	T11096	T11082	punct	", ",implies
R7321	T11097	T11098	mark	that,is
R7322	T11098	T11082	ccomp	is,implies
R7323	T11099	T11100	det	the,threshold
R7324	T11100	T11098	nsubj	threshold,is
R7325	T11101	T11100	prep	for,threshold
R7326	T11102	T11101	pobj	induction,for
R7327	T11103	T11102	prep	of,induction
R7328	T11104	T11103	pobj	apoptosis,of
R7329	T11105	T11098	acomp	low,is
R733	T1644	T1643	amod	early,bud
R7330	T11106	T11105	advmod	enough,low
R7331	T11107	T11108	mark	that,leaves
R7332	T11108	T11105	ccomp	leaves,low
R7333	T11109	T11108	nsubj	loss,leaves
R7334	T11110	T11109	prep	of,loss
R7335	T11111	T11110	pobj	BMP2,of
R7336	T11112	T11111	punct	", ",BMP2
R7337	T11113	T11111	conj	BMP4,BMP2
R7338	T11114	T11113	cc	or,BMP4
R7339	T11115	T11113	conj	BMP7,BMP4
R734	T1645	T1643	compound	limb,bud
R7340	T11116	T11111	cc	or,BMP2
R7341	T11117	T11111	conj	BMP2,BMP2
R7342	T11118	T11117	cc	and,BMP2
R7343	T11119	T11117	conj	BMP7,BMP2
R7344	T11120	T11108	advmod	still,leaves
R7345	T11121	T11122	amod	sufficient,signaling
R7346	T11122	T11108	dobj	signaling,leaves
R7347	T11123	T11122	compound	BMP,signaling
R7348	T11124	T11125	mark	for,occur
R7349	T11125	T11122	advcl	occur,signaling
R735	T1646	T1617	punct	.,expressed
R7350	T11126	T11127	amod	most,death
R7351	T11127	T11125	nsubj	death,occur
R7352	T11128	T11127	amod	interdigital,death
R7353	T11129	T11127	compound	cell,death
R7354	T11130	T11125	aux	to,occur
R7355	T11131	T11125	advmod	normally,occur
R7356	T11132	T11082	punct	.,implies
R7357	T11134	T11135	advmod	However,results
R7358	T11136	T11135	punct	", ",results
R7359	T11137	T11135	nsubj	loss,results
R736	T1648	T1649	prep	Like,expressed
R7360	T11138	T11137	prep	of,loss
R7361	T11139	T11140	preconj	both,BMP2
R7362	T11140	T11138	pobj	BMP2,of
R7363	T11141	T11140	cc	and,BMP2
R7364	T11142	T11140	conj	BMP4,BMP2
R7365	T11143	T11135	prep	in,results
R7366	T11144	T11145	amod	complete,syndactyly
R7367	T11145	T11143	pobj	syndactyly,in
R7368	T11146	T11147	amod	soft,tissue
R7369	T11147	T11145	compound	tissue,syndactyly
R737	T1649	T1654	ccomp	expressed,appear
R7370	T11148	T11145	punct	", ",syndactyly
R7371	T11149	T11150	advmod	i.e.,webbing
R7372	T11150	T11145	appos	webbing,syndactyly
R7373	T11151	T11150	punct	", ",webbing
R7374	T11152	T11150	prep	between,webbing
R7375	T11153	T11154	predet	all,digits
R7376	T11154	T11152	pobj	digits,between
R7377	T11155	T11154	det	the,digits
R7378	T11156	T11135	punct	.,results
R7379	T11158	T11159	mark	Although,implicated
R738	T1650	T1648	pobj	BMP7,Like
R7380	T11159	T11165	advcl	implicated,is
R7381	T11160	T11161	compound	BMP,activity
R7382	T11161	T11159	nsubjpass	activity,implicated
R7383	T11162	T11159	aux	has,implicated
R7384	T11163	T11159	auxpass	been,implicated
R7385	T11164	T11159	advmod	previously,implicated
R7386	T11166	T11159	prep	in,implicated
R7387	T11167	T11168	amod	regulating,apoptosis
R7388	T11168	T11166	pobj	apoptosis,in
R7389	T11169	T11168	amod	interdigital,apoptosis
R739	T1651	T1649	punct	", ",expressed
R7390	T11170	T11165	punct	", ",is
R7391	T11171	T11165	nsubj	this,is
R7392	T11172	T11173	det	the,verification
R7393	T11173	T11165	attr	verification,is
R7394	T11174	T11173	amod	first,verification
R7395	T11175	T11173	amod	genetic,verification
R7396	T11176	T11173	prep	of,verification
R7397	T11177	T11178	poss	their,requirement
R7398	T11178	T11176	pobj	requirement,of
R7399	T11179	T11165	prep	for,is
R74	T235	T233	pobj	redundancy,with
R740	T1652	T1649	nsubjpass	it,expressed
R7400	T11180	T11181	det	this,process
R7401	T11181	T11179	pobj	process,for
R7402	T11182	T11165	punct	.,is
R7404	T11683	T11684	compound	BMP,Signaling
R7405	T11685	T11684	cc	and,Signaling
R7406	T11686	T11687	amod	Skeletal,Development
R7407	T11687	T11684	conj	Development,Signaling
R7408	T11689	T11690	mark	As,discussed
R7409	T11690	T11691	advcl	discussed,indicate
R741	T1653	T1649	auxpass	is,expressed
R7410	T11692	T11690	advmod	above,discussed
R7411	T11693	T11691	punct	", ",indicate
R7412	T11694	T11695	poss	our,data
R7413	T11695	T11691	nsubj	data,indicate
R7414	T11696	T11697	mark	that,required
R7415	T11697	T11691	ccomp	required,indicate
R7416	T11698	T11699	compound	threshold,levels
R7417	T11699	T11697	nsubjpass	levels,required
R7418	T11700	T11699	prep	of,levels
R7419	T11701	T11702	compound	BMP,signaling
R742	T1655	T1649	prep	in,expressed
R7420	T11702	T11700	pobj	signaling,of
R7421	T11703	T11697	auxpass	are,required
R7422	T11704	T11697	prep	for,required
R7423	T11705	T11704	pcomp	initiating,for
R7424	T11706	T11707	amod	chondrogenic,condensation
R7425	T11707	T11705	dobj	condensation,initiating
R7426	T11708	T11691	punct	", ",indicate
R7427	T11709	T11691	advcl	consistent,indicate
R7428	T11710	T11709	prep	with,consistent
R7429	T11711	T11712	amod	prior,results
R743	T1656	T1657	preconj	both,margins
R7430	T11712	T11710	pobj	results,with
R7431	T11713	T11714	punct	[,26
R7432	T11714	T11691	parataxis	26,indicate
R7433	T11715	T11716	punct	–,28
R7434	T11716	T11714	prep	28,26
R7435	T11717	T11714	punct	],26
R7436	T11718	T11691	punct	.,indicate
R7437	T11720	T11721	advmod	However,sustained
R7438	T11722	T11721	punct	", ",sustained
R7439	T11723	T11724	mark	once,begins
R744	T1657	T1655	pobj	margins,in
R7440	T11724	T11721	advcl	begins,sustained
R7441	T11725	T11724	nsubj	chondrogenesis,begins
R7442	T11726	T11721	punct	", ",sustained
R7443	T11727	T11728	compound	cartilage,differentiation
R7444	T11728	T11721	nsubjpass	differentiation,sustained
R7445	T11729	T11721	aux	can,sustained
R7446	T11730	T11721	auxpass	be,sustained
R7447	T11731	T11721	punct	", ",sustained
R7448	T11732	T11733	dep	albeit,with
R7449	T11733	T11721	prep	with,sustained
R745	T1658	T1657	det	the,margins
R7450	T11734	T11735	det	some,delay
R7451	T11735	T11733	pobj	delay,with
R7452	T11736	T11735	amod	detectable,delay
R7453	T11737	T11721	punct	", ",sustained
R7454	T11738	T11721	prep	in,sustained
R7455	T11739	T11740	det	the,absence
R7456	T11740	T11738	pobj	absence,in
R7457	T11741	T11740	prep	of,absence
R7458	T11742	T11741	pobj	BMP2,of
R7459	T11743	T11742	cc	and,BMP2
R746	T1659	T1657	amod	anterior,margins
R7460	T11744	T11742	conj	BMP4,BMP2
R7461	T11745	T11738	cc	or,in
R7462	T11746	T11738	conj	in,in
R7463	T11747	T11748	det	the,absence
R7464	T11748	T11746	pobj	absence,in
R7465	T11749	T11748	prep	of,absence
R7466	T11750	T11749	pobj	BMP2,of
R7467	T11751	T11750	cc	and,BMP2
R7468	T11752	T11750	conj	BMP7,BMP2
R7469	T11753	T11721	punct	.,sustained
R747	T1660	T1659	cc	and,anterior
R7470	T11755	T11756	advmod	Thus,is
R7471	T11757	T11756	punct	", ",is
R7472	T11758	T11759	det	the,expression
R7473	T11759	T11756	nsubj	expression,is
R7474	T11760	T11759	prep	of,expression
R7475	T11761	T11762	preconj	either,BMP5
R7476	T11762	T11760	pobj	BMP5,of
R7477	T11763	T11762	punct	", ",BMP5
R7478	T11764	T11762	conj	BMP6,BMP5
R7479	T11765	T11764	punct	", ",BMP6
R748	T1661	T1659	conj	posterior,anterior
R7480	T11766	T11764	cc	and,BMP6
R7481	T11767	T11764	conj	BMP7,BMP6
R7482	T11768	T11762	punct	(,BMP5
R7483	T11769	T11762	prep	in,BMP5
R7484	T11770	T11771	det	the,absence
R7485	T11771	T11769	pobj	absence,in
R7486	T11772	T11771	prep	of,absence
R7487	T11773	T11772	pobj	BMP2,of
R7488	T11774	T11773	cc	and,BMP2
R7489	T11775	T11773	conj	BMP4,BMP2
R749	T1662	T1657	prep	of,margins
R7490	T11776	T11762	punct	),BMP5
R7491	T11777	T11762	cc	or,BMP5
R7492	T11778	T11779	det	the,expression
R7493	T11779	T11762	conj	expression,BMP5
R7494	T11780	T11779	prep	of,expression
R7495	T11781	T11780	pobj	BMP4,of
R7496	T11782	T11781	punct	", ",BMP4
R7497	T11783	T11781	conj	BMP5,BMP4
R7498	T11784	T11783	punct	", ",BMP5
R7499	T11785	T11783	cc	and,BMP5
R75	T236	T235	prep	among,redundancy
R750	T1663	T1664	det	the,mesenchyme
R7500	T11786	T11783	conj	BMP6,BMP5
R7501	T11787	T11779	punct	(,expression
R7502	T11788	T11779	prep	in,expression
R7503	T11789	T11790	det	the,absence
R7504	T11790	T11788	pobj	absence,in
R7505	T11791	T11790	prep	of,absence
R7506	T11792	T11791	pobj	BMP2,of
R7507	T11793	T11792	cc	and,BMP2
R7508	T11794	T11792	conj	BMP7,BMP2
R7509	T11795	T11756	punct	),is
R751	T1664	T1662	pobj	mesenchyme,of
R7510	T11796	T11756	acomp	sufficient,is
R7511	T11797	T11798	aux	to,support
R7512	T11798	T11796	xcomp	support,sufficient
R7513	T11799	T11800	amod	mesenchymal,condensation
R7514	T11800	T11798	dobj	condensation,support
R7515	T11801	T11800	cc	and,condensation
R7516	T11802	T11803	compound	chondrocyte,differentiation
R7517	T11803	T11800	conj	differentiation,condensation
R7518	T11804	T11798	prep	in,support
R7519	T11805	T11806	det	the,limb
R752	T1665	T1666	compound	limb,bud
R7520	T11806	T11804	pobj	limb,in
R7521	T11807	T11806	amod	developing,limb
R7522	T11808	T11756	punct	.,is
R7523	T11810	T11811	prep	In,showed
R7524	T11812	T11813	nummod	one,set
R7525	T11813	T11810	pobj	set,In
R7526	T11814	T11813	prep	of,set
R7527	T11815	T11816	amod	previous,studies
R7528	T11816	T11814	pobj	studies,of
R7529	T11817	T11813	acl	implicating,set
R753	T1666	T1664	compound	bud,mesenchyme
R7530	T11818	T11819	compound	BMP,signaling
R7531	T11819	T11817	dobj	signaling,implicating
R7532	T11820	T11819	prep	in,signaling
R7533	T11821	T11820	pobj	chondrogenesis,in
R7534	T11822	T11811	punct	", ",showed
R7535	T11823	T11811	nsubj	Yoon,showed
R7536	T11824	T11825	advmod	et,al.
R7537	T11825	T11823	advmod	al.,Yoon
R7538	T11826	T11827	punct	[,26
R7539	T11827	T11823	parataxis	26,Yoon
R754	T1667	T1668	punct	[,14
R7540	T11828	T11827	punct	],26
R7541	T11829	T11830	mark	that,is
R7542	T11830	T11811	ccomp	is,showed
R7543	T11831	T11832	mark	if,lose
R7544	T11832	T11830	advcl	lose,is
R7545	T11833	T11832	nsubj	mice,lose
R7546	T11834	T11835	preconj	both,Alk3
R7547	T11835	T11832	dobj	Alk3,lose
R7548	T11836	T11835	punct	(,Alk3
R7549	T11837	T11835	appos	BmprIa,Alk3
R755	T1668	T1649	parataxis	14,expressed
R7550	T11838	T11835	punct	),Alk3
R7551	T11839	T11835	cc	and,Alk3
R7552	T11840	T11835	conj	Alk6,Alk3
R7553	T11841	T11840	punct	(,Alk6
R7554	T11842	T11840	appos	BmpIb,Alk6
R7555	T11843	T11835	punct	),Alk3
R7556	T11844	T11835	punct	", ",Alk3
R7557	T11845	T11835	appos	two,Alk3
R7558	T11846	T11845	prep	of,two
R7559	T11847	T11848	det	the,receptors
R756	T1669	T1668	nummod	4,14
R7560	T11848	T11846	pobj	receptors,of
R7561	T11849	T11848	nummod	three,receptors
R7562	T11850	T11848	nmod	type,receptors
R7563	T11851	T11850	nummod	I,type
R7564	T11852	T11848	acl	used,receptors
R7565	T11853	T11852	prep	in,used
R7566	T11854	T11855	compound	BMP,transduction
R7567	T11855	T11853	pobj	transduction,in
R7568	T11856	T11855	compound	signal,transduction
R7569	T11857	T11830	punct	", ",is
R757	T1670	T1668	punct	",",14
R7570	T11858	T11830	expl	there,is
R7571	T11859	T11860	det	a,decrease
R7572	T11860	T11830	attr	decrease,is
R7573	T11861	T11860	amod	dramatic,decrease
R7574	T11862	T11860	prep	in,decrease
R7575	T11863	T11864	det	the,size
R7576	T11864	T11862	pobj	size,in
R7577	T11865	T11864	prep	of,size
R7578	T11866	T11867	amod	skeletal,primordial
R7579	T11867	T11865	pobj	primordial,of
R758	T1671	T1668	punct	],14
R7580	T11868	T11830	prep	due,is
R7581	T11869	T11868	pcomp	to,due
R7582	T11870	T11871	det	a,reduction
R7583	T11871	T11868	pobj	reduction,due
R7584	T11872	T11871	prep	of,reduction
R7585	T11873	T11872	pobj	proliferation,of
R7586	T11874	T11871	cc	and,reduction
R7587	T11875	T11871	conj	increase,reduction
R7588	T11876	T11875	prep	in,increase
R7589	T11877	T11876	pobj	apoptosis,in
R759	T1672	T1654	punct	;,appear
R7590	T11878	T11811	punct	.,showed
R7591	T11880	T11881	det	The,size
R7592	T11881	T11882	nsubjpass	size,decreased
R7593	T11883	T11881	prep	of,size
R7594	T11884	T11885	det	the,elements
R7595	T11885	T11883	pobj	elements,of
R7596	T11886	T11885	amod	skeletal,elements
R7597	T11887	T11885	prep	in,elements
R7598	T11888	T11889	nmod	Bmp2C,C
R7599	T11889	T11891	nmod	C,limbs
R76	T237	T238	compound	BMP,molecules
R760	T1673	T1654	advmod	however,appear
R7600	T11890	T11889	punct	/,C
R7601	T11891	T11887	pobj	limbs,in
R7602	T11892	T11889	punct	;,C
R7603	T11893	T11894	compound	Bmp4C,C
R7604	T11894	T11889	appos	C,C
R7605	T11895	T11894	punct	/,C
R7606	T11896	T11889	punct	;,C
R7607	T11897	T11898	compound	Prx1,cre
R7608	T11898	T11889	appos	cre,C
R7609	T11899	T11898	punct	:,cre
R761	T1674	T1654	punct	", ",appear
R7610	T11900	T11898	punct	:,cre
R7611	T11901	T11882	auxpass	are,decreased
R7612	T11902	T11882	advmod	also,decreased
R7613	T11903	T11882	prep	compared,decreased
R7614	T11904	T11903	prep	to,compared
R7615	T11905	T11904	pobj	those,to
R7616	T11906	T11905	prep	of,those
R7617	T11907	T11908	compound	control,mice
R7618	T11908	T11906	pobj	mice,of
R7619	T11909	T11882	punct	", ",decreased
R762	T1675	T1654	nsubj	it,appear
R7620	T11910	T11911	mark	although,to
R7621	T11911	T11882	advcl	to,decreased
R7622	T11912	T11911	neg	not,to
R7623	T11913	T11914	det	the,extent
R7624	T11914	T11911	pobj	extent,to
R7625	T11915	T11914	acl	seen,extent
R7626	T11916	T11915	prep	in,seen
R7627	T11917	T11918	det	the,mutant
R7628	T11918	T11916	pobj	mutant,in
R7629	T11919	T11918	amod	double,mutant
R763	T1676	T1654	aux	does,appear
R7630	T11920	T11918	compound	receptor,mutant
R7631	T11921	T11882	punct	.,decreased
R7632	T11923	T11924	nsubj	We,know
R7633	T11925	T11924	aux	do,know
R7634	T11926	T11924	neg	not,know
R7635	T11927	T11928	mark	if,is
R7636	T11928	T11924	ccomp	is,know
R7637	T11929	T11930	amod	reduced,size
R7638	T11930	T11928	nsubj	size,is
R7639	T11931	T11930	prep	of,size
R764	T1677	T1654	neg	not,appear
R7640	T11932	T11933	det	the,elements
R7641	T11933	T11931	pobj	elements,of
R7642	T11934	T11933	amod	skeletal,elements
R7643	T11935	T11933	prep	in,elements
R7644	T11936	T11937	poss	our,study
R7645	T11937	T11935	pobj	study,in
R7646	T11938	T11928	advmod	similarly,is
R7647	T11939	T11928	prep	due,is
R7648	T11940	T11939	pcomp	to,due
R7649	T11941	T11942	amod	reduced,proliferation
R765	T1678	T1679	aux	to,induced
R7650	T11942	T11939	pobj	proliferation,due
R7651	T11943	T11942	cc	and,proliferation
R7652	T11944	T11942	conj	increase,proliferation
R7653	T11945	T11944	prep	in,increase
R7654	T11946	T11945	pobj	apoptosis,in
R7655	T11947	T11942	punct	", ",proliferation
R7656	T11948	T11942	conj	lack,proliferation
R7657	T11949	T11948	prep	of,lack
R7658	T11950	T11949	pobj	replacement,of
R7659	T11951	T11948	prep	by,lack
R766	T1679	T1654	xcomp	induced,appear
R7660	T11952	T11951	pobj	bone,by
R7661	T11953	T11948	punct	", ",lack
R7662	T11954	T11948	cc	or,lack
R7663	T11955	T11956	det	a,combination
R7664	T11956	T11948	conj	combination,lack
R7665	T11957	T11956	prep	of,combination
R7666	T11958	T11957	pobj	these,of
R7667	T11959	T11924	punct	.,know
R7668	T11961	T11962	nsubj	It,is
R7669	T11963	T11962	acomp	possible,is
R767	T1680	T1679	auxpass	be,induced
R7670	T11964	T11965	mark	that,have
R7671	T11965	T11962	ccomp	have,is
R7672	T11966	T11967	det	a,mutant
R7673	T11967	T11965	nsubj	mutant,have
R7674	T11968	T11967	amod	triple,mutant
R7675	T11969	T11967	acl	removing,mutant
R7676	T11970	T11971	nmod	BMP2,activities
R7677	T11971	T11969	dobj	activities,removing
R7678	T11972	T11970	punct	", ",BMP2
R7679	T11973	T11970	conj	BMP4,BMP2
R768	T1681	T1679	prep	by,induced
R7680	T11974	T11973	punct	", ",BMP4
R7681	T11975	T11973	cc	and,BMP4
R7682	T11976	T11973	conj	BMP7,BMP4
R7683	T11977	T11965	aux	would,have
R7684	T11978	T11979	det	a,phenotype
R7685	T11979	T11965	dobj	phenotype,have
R7686	T11980	T11979	amod	similar,phenotype
R7687	T11981	T11979	prep	as,phenotype
R7688	T11982	T11983	det	the,mutant
R7689	T11983	T11981	pobj	mutant,as
R769	T1682	T1683	compound	SHH,signaling
R7690	T11984	T11985	nmod	BmprIa,BmprIb
R7691	T11985	T11983	nmod	BmprIb,mutant
R7692	T11986	T11985	punct	", ",BmprIb
R7693	T11987	T11983	amod	double,mutant
R7694	T11988	T11965	punct	", ",have
R7695	T11989	T11990	mark	although,compensated
R7696	T11990	T11965	advcl	compensated,have
R7697	T11991	T11990	nsubjpass	these,compensated
R7698	T11992	T11990	aux	could,compensated
R7699	T11993	T11990	advmod	also,compensated
R77	T238	T236	pobj	molecules,among
R770	T1683	T1681	pobj	signaling,by
R7700	T11994	T11990	auxpass	be,compensated
R7701	T11995	T11990	prep	from,compensated
R7702	T11996	T11997	amod	other,BMPs
R7703	T11997	T11995	pobj	BMPs,from
R7704	T11998	T11962	punct	.,is
R7705	T12000	T12001	nsubj	We,know
R7706	T12002	T12001	aux	do,know
R7707	T12003	T12001	punct	", ",know
R7708	T12004	T12001	advmod	however,know
R7709	T12005	T12001	punct	", ",know
R771	T1684	T1654	punct	", ",appear
R7710	T12006	T12007	mark	that,is
R7711	T12007	T12001	ccomp	is,know
R7712	T12008	T12007	nsubj	osteogenesis,is
R7713	T12009	T12007	acomp	defective,is
R7714	T12010	T12007	prep	in,is
R7715	T12011	T12012	det	these,mice
R7716	T12012	T12010	pobj	mice,in
R7717	T12013	T12007	punct	", ",is
R7718	T12014	T12015	mark	although,proceeds
R7719	T12015	T12007	advcl	proceeds,is
R772	T1685	T1654	cc	nor,appear
R7720	T12016	T12017	compound	bone,formation
R7721	T12017	T12015	nsubj	formation,proceeds
R7722	T12018	T12015	advmod	normally,proceeds
R7723	T12019	T12015	prep	in,proceeds
R7724	T12020	T12019	pobj	siblings,in
R7725	T12021	T12020	acl	retaining,siblings
R7726	T12022	T12023	nummod	one,copy
R7727	T12023	T12021	dobj	copy,retaining
R7728	T12024	T12023	amod	functional,copy
R7729	T12025	T12023	prep	of,copy
R773	T1686	T1687	aux	does,change
R7730	T12026	T12027	preconj	either,gene
R7731	T12027	T12025	pobj	gene,of
R7732	T12028	T12027	det	the,gene
R7733	T12029	T12027	nmod	Bmp2,gene
R7734	T12030	T12029	cc	or,Bmp2
R7735	T12031	T12029	conj	Bmp4,Bmp2
R7736	T12032	T12033	punct	(,S4
R7737	T12033	T12021	parataxis	S4,retaining
R7738	T12034	T12033	compound	Figure,S4
R7739	T12035	T12033	punct	),S4
R774	T1687	T1654	conj	change,appear
R7740	T12036	T12021	punct	", ",retaining
R7741	T12037	T12038	mark	as,S2
R7742	T12038	T12021	advcl	S2,retaining
R7743	T12039	T12038	nsubj	it,S2
R7744	T12040	T12038	aux	does,S2
R7745	T12041	T12038	prep	in,S2
R7746	T12042	T12041	pobj	mice,in
R7747	T12043	T12044	advmod	completely,deficient
R7748	T12044	T12042	amod	deficient,mice
R7749	T12045	T12044	prep	in,deficient
R775	T1688	T1689	poss	its,expression
R7750	T12046	T12045	pobj	BMP2,in
R7751	T12047	T12046	cc	and,BMP2
R7752	T12048	T12046	conj	BMP7,BMP2
R7753	T12049	T12038	punct	(,S2
R7754	T12050	T12038	nmod	Figures,S2
R7755	T12051	T12038	nummod	1,S2
R7756	T12052	T12038	cc	and,S2
R7757	T12053	T12038	punct	),S2
R7758	T12054	T12001	punct	.,know
R7759	T12056	T12057	det	The,phenotype
R776	T1689	T1687	nsubj	expression,change
R7760	T12057	T12058	nsubj	phenotype,is
R7761	T12059	T12060	nsubj	we,observe
R7762	T12060	T12057	advcl	observe,phenotype
R7763	T12061	T12060	prep	in,observe
R7764	T12062	T12061	pobj	limbs,in
R7765	T12063	T12062	prep	of,limbs
R7766	T12064	T12065	nmod	Bmp2C,C
R7767	T12065	T12067	nmod	C,mice
R7768	T12066	T12065	punct	/,C
R7769	T12067	T12063	pobj	mice,of
R777	T1690	T1687	prep	in,change
R7770	T12068	T12065	punct	;,C
R7771	T12069	T12070	compound	Bmp4C,C
R7772	T12070	T12065	appos	C,C
R7773	T12071	T12070	punct	/,C
R7774	T12072	T12065	punct	;,C
R7775	T12073	T12074	compound	Prx1,cre
R7776	T12074	T12065	appos	cre,C
R7777	T12075	T12074	punct	:,cre
R7778	T12076	T12074	punct	:,cre
R7779	T12077	T12058	acomp	similar,is
R778	T1691	T1692	npadvmod	SHH,deficient
R7780	T12078	T12077	prep	to,similar
R7781	T12079	T12080	det	the,phenotype
R7782	T12080	T12078	pobj	phenotype,to
R7783	T12081	T12080	acl	reported,phenotype
R7784	T12082	T12081	prep	for,reported
R7785	T12083	T12084	compound	osterix,knockout
R7786	T12084	T12085	compound	knockout,mice
R7787	T12085	T12082	pobj	mice,for
R7788	T12086	T12087	punct	[,46
R7789	T12087	T12080	parataxis	46,phenotype
R779	T1692	T1694	amod	deficient,buds
R7790	T12088	T12087	punct	],46
R7791	T12089	T12080	punct	", ",phenotype
R7792	T12090	T12091	dep	which,exhibited
R7793	T12091	T12080	relcl	exhibited,phenotype
R7794	T12092	T12093	amod	severe,defects
R7795	T12093	T12091	dobj	defects,exhibited
R7796	T12094	T12093	prep	in,defects
R7797	T12095	T12096	amod	osteoblastic,differentiation
R7798	T12096	T12094	pobj	differentiation,in
R7799	T12097	T12058	punct	.,is
R78	T239	T215	punct	", ",been
R780	T1693	T1692	punct	-,deficient
R7800	T12099	T12100	mark	Since,observe
R7801	T12100	T12102	advcl	observe,be
R7802	T12101	T12100	nsubj	we,observe
R7803	T12103	T12104	amod	fibroblastic,cells
R7804	T12104	T12100	dobj	cells,observe
R7805	T12105	T12104	amod	adjacent,cells
R7806	T12106	T12105	prep	to,adjacent
R7807	T12107	T12108	amod	mineralized,cartilage
R7808	T12108	T12106	pobj	cartilage,to
R7809	T12109	T12110	dep	that,have
R781	T1694	T1690	pobj	buds,in
R7810	T12110	T12104	relcl	have,cells
R7811	T12111	T12110	dobj	characteristics,have
R7812	T12112	T12111	prep	of,characteristics
R7813	T12113	T12114	compound	osteoprogenitor,cells
R7814	T12114	T12112	pobj	cells,of
R7815	T12115	T12110	cc	but,have
R7816	T12116	T12110	conj	fail,have
R7817	T12117	T12118	aux	to,express
R7818	T12118	T12116	xcomp	express,fail
R7819	T12119	T12118	dobj	osterix,express
R782	T1695	T1694	compound	limb,buds
R7820	T12120	T12118	prep	in,express
R7821	T12121	T12122	det	the,absence
R7822	T12122	T12120	pobj	absence,in
R7823	T12123	T12122	prep	of,absence
R7824	T12124	T12125	preconj	both,BMP2
R7825	T12125	T12123	pobj	BMP2,of
R7826	T12126	T12125	cc	and,BMP2
R7827	T12127	T12125	conj	BMP4,BMP2
R7828	T12128	T12102	punct	", ",be
R7829	T12129	T12130	nummod	one,role
R783	T1696	T1654	punct	.,appear
R7830	T12130	T12102	nsubj	role,be
R7831	T12131	T12130	amod	important,role
R7832	T12132	T12130	prep	of,role
R7833	T12133	T12132	pobj	BMP2,of
R7834	T12134	T12133	cc	and,BMP2
R7835	T12135	T12133	conj	BMP4,BMP2
R7836	T12136	T12130	prep	during,role
R7837	T12137	T12138	amod	endochondral,ossification
R7838	T12138	T12136	pobj	ossification,during
R7839	T12139	T12102	aux	may,be
R784	T1698	T1699	advmod	Thus,is
R7840	T12140	T12141	aux	to,induce
R7841	T12141	T12102	xcomp	induce,be
R7842	T12142	T12143	compound	osterix,expression
R7843	T12143	T12141	dobj	expression,induce
R7844	T12144	T12143	compound	gene,expression
R7845	T12145	T12141	prep	in,induce
R7846	T12146	T12145	pobj	osteoprogenitors,in
R7847	T12147	T12102	punct	.,be
R7848	T12149	T12150	advmod	Strikingly,observe
R7849	T12151	T12150	punct	", ",observe
R785	T1700	T1699	punct	", ",is
R7850	T12152	T12150	advmod	however,observe
R7851	T12153	T12150	punct	", ",observe
R7852	T12154	T12150	nsubj	we,observe
R7853	T12155	T12150	aux	do,observe
R7854	T12156	T12157	compound	osterix,expression
R7855	T12157	T12150	dobj	expression,observe
R7856	T12158	T12157	prep	in,expression
R7857	T12159	T12160	det	the,collar
R7858	T12160	T12158	pobj	collar,in
R7859	T12161	T12160	compound	bone,collar
R786	T1701	T1699	nsubj	BMP4,is
R7860	T12162	T12160	prep	of,collar
R7861	T12163	T12164	compound	E16.5,mice
R7862	T12164	T12162	pobj	mice,of
R7863	T12165	T12164	acl	missing,mice
R7864	T12166	T12167	amod	functional,copies
R7865	T12167	T12165	dobj	copies,missing
R7866	T12168	T12167	prep	of,copies
R7867	T12169	T12170	preconj	both,genes
R7868	T12170	T12168	pobj	genes,of
R7869	T12171	T12170	det	the,genes
R787	T1702	T1699	neg	not,is
R7870	T12172	T12170	nmod	Bmp2,genes
R7871	T12173	T12172	cc	and,Bmp2
R7872	T12174	T12172	conj	Bmp4,Bmp2
R7873	T12175	T12176	punct	(,K.
R7874	T12176	T12150	meta	K.,observe
R7875	T12177	T12176	nmod	Tsuji,K.
R7876	T12178	T12176	cc	and,K.
R7877	T12179	T12176	conj	A.,K.
R7878	T12180	T12179	nmod	Bandyopadhyay,A.
R7879	T12181	T12179	punct	", ",A.
R788	T1703	T1704	det	a,candidate
R7880	T12182	T12183	amod	unpublished,data
R7881	T12183	T12179	conj	data,A.
R7882	T12184	T12183	punct	),data
R7883	T12185	T12150	punct	.,observe
R7884	T12187	T12188	nsubj	This,suggests
R7885	T12189	T12190	mark	that,result
R7886	T12190	T12188	ccomp	result,suggests
R7887	T12191	T12192	amod	varying,levels
R7888	T12192	T12190	nsubj	levels,result
R7889	T12193	T12192	prep	of,levels
R789	T1704	T1699	attr	candidate,is
R7890	T12194	T12193	pobj	expression,of
R7891	T12195	T12192	prep	from,levels
R7892	T12196	T12197	amod	different,genes
R7893	T12197	T12195	pobj	genes,from
R7894	T12198	T12197	compound	BMP,genes
R7895	T12199	T12192	prep	during,levels
R7896	T12200	T12201	amod	preaxial,development
R7897	T12201	T12199	pobj	development,during
R7898	T12202	T12200	cc	and,preaxial
R7899	T12203	T12200	conj	postaxial,preaxial
R79	T240	T215	nsubj	it,been
R790	T1705	T1704	prep	for,candidate
R7900	T12204	T12190	prep	in,result
R7901	T12205	T12204	pobj	fluctuations,in
R7902	T12206	T12205	prep	in,fluctuations
R7903	T12207	T12208	amod	total,levels
R7904	T12208	T12206	pobj	levels,in
R7905	T12209	T12208	prep	of,levels
R7906	T12210	T12211	compound	BMP,signaling
R7907	T12211	T12209	pobj	signaling,of
R7908	T12212	T12208	punct	", ",levels
R7909	T12213	T12214	dep	which,are
R791	T1706	T1707	det	a,signal
R7910	T12214	T12208	relcl	are,levels
R7911	T12215	T12214	prep	at,are
R7912	T12216	T12217	amod	distinct,times
R7913	T12217	T12215	pobj	times,at
R7914	T12218	T12214	prep	above,are
R7915	T12219	T12218	cc	or,above
R7916	T12220	T12218	conj	below,above
R7917	T12221	T12222	det	the,threshold
R7918	T12222	T12220	pobj	threshold,below
R7919	T12223	T12222	prep	for,threshold
R792	T1707	T1705	pobj	signal,for
R7920	T12224	T12223	pcomp	supporting,for
R7921	T12225	T12224	dobj	osteogenesis,supporting
R7922	T12226	T12188	punct	.,suggests
R7923	T12228	T12229	mark	While,expressed
R7924	T12229	T12251	advcl	expressed,suggest
R7925	T12230	T12231	amod	many,molecules
R7926	T12231	T12229	nsubjpass	molecules,expressed
R7927	T12232	T12231	amod	osteogenic,molecules
R7928	T12233	T12231	compound	BMP,molecules
R7929	T12234	T12231	punct	", ",molecules
R793	T1708	T1707	amod	secondary,signal
R7930	T12235	T12231	advmod	apart,molecules
R7931	T12236	T12235	prep	from,apart
R7932	T12237	T12236	pobj	BMP2,from
R7933	T12238	T12237	cc	and,BMP2
R7934	T12239	T12237	conj	BMP4,BMP2
R7935	T12240	T12231	punct	", ",molecules
R7936	T12241	T12242	amod	such,as
R7937	T12242	T12231	prep	as,molecules
R7938	T12243	T12242	pobj	BMP5,as
R7939	T12244	T12243	punct	", ",BMP5
R794	T1709	T1707	advmod	downstream,signal
R7940	T12245	T12243	conj	BMP6,BMP5
R7941	T12246	T12245	punct	", ",BMP6
R7942	T12247	T12245	cc	and,BMP6
R7943	T12248	T12245	conj	BMP7,BMP6
R7944	T12249	T12229	punct	", ",expressed
R7945	T12250	T12229	auxpass	are,expressed
R7946	T12252	T12229	prep	during,expressed
R7947	T12253	T12254	det	the,process
R7948	T12254	T12252	pobj	process,during
R7949	T12255	T12254	amod	endochondral,process
R795	T1710	T1709	prep	of,downstream
R7950	T12256	T12251	punct	", ",suggest
R7951	T12257	T12258	poss	our,data
R7952	T12258	T12251	nsubj	data,suggest
R7953	T12259	T12260	mark	that,compensate
R7954	T12260	T12251	ccomp	compensate,suggest
R7955	T12261	T12262	det	these,molecules
R7956	T12262	T12260	nsubj	molecules,compensate
R7957	T12263	T12262	compound	BMP,molecules
R7958	T12264	T12260	aux	can,compensate
R7959	T12265	T12260	neg	not,compensate
R796	T1711	T1710	pobj	SHH,of
R7960	T12266	T12260	prep	for,compensate
R7961	T12267	T12268	det	the,loss
R7962	T12268	T12266	pobj	loss,for
R7963	T12269	T12268	amod	combined,loss
R7964	T12270	T12268	prep	of,loss
R7965	T12271	T12270	pobj	BMP2,of
R7966	T12272	T12271	cc	and,BMP2
R7967	T12273	T12271	conj	BMP4,BMP2
R7968	T12274	T12268	prep	in,loss
R7969	T12275	T12276	compound	bone,formation
R797	T1712	T1699	prep	in,is
R7970	T12276	T12274	pobj	formation,in
R7971	T12277	T12251	punct	.,suggest
R7972	T12279	T12280	amod	Recent,experiments
R7973	T12280	T12284	nsubj	experiments,suggested
R7974	T12281	T12280	amod	biochemical,experiments
R7975	T12282	T12281	cc	and,biochemical
R7976	T12283	T12281	conj	genetic,biochemical
R7977	T12285	T12284	aux	have,suggested
R7978	T12286	T12287	mark	that,have
R7979	T12287	T12284	ccomp	have,suggested
R798	T1713	T1714	amod	early,patterning
R7980	T12288	T12289	amod	individual,BMPs
R7981	T12289	T12287	nsubj	BMPs,have
R7982	T12290	T12291	amod	identical,functions
R7983	T12291	T12287	dobj	functions,have
R7984	T12292	T12284	punct	.,suggested
R7985	T12294	T12295	nsubj	BMP2,utilize
R7986	T12296	T12294	punct	", ",BMP2
R7987	T12297	T12294	conj	BMP4,BMP2
R7988	T12298	T12297	punct	", ",BMP4
R7989	T12299	T12297	conj	BMP5,BMP4
R799	T1714	T1712	pobj	patterning,in
R7990	T12300	T12299	punct	", ",BMP5
R7991	T12301	T12299	conj	BMP6,BMP5
R7992	T12302	T12301	punct	", ",BMP6
R7993	T12303	T12301	cc	and,BMP6
R7994	T12304	T12301	conj	BMP7,BMP6
R7995	T12305	T12295	aux	can,utilize
R7996	T12306	T12307	det	the,receptors
R7997	T12307	T12295	dobj	receptors,utilize
R7998	T12308	T12307	amod	same,receptors
R7999	T12309	T12307	nmod	type,receptors
R8	T162	T161	punct	", ",BMP4
R80	T241	T215	aux	has,been
R800	T1715	T1699	punct	.,is
R8000	T12310	T12309	nummod	I,type
R8001	T12311	T12312	punct	(,Alk2
R8002	T12312	T12309	parataxis	Alk2,type
R8003	T12313	T12312	punct	", ",Alk2
R8004	T12314	T12312	conj	Alk3,Alk2
R8005	T12315	T12314	punct	", ",Alk3
R8006	T12316	T12314	cc	and,Alk3
R8007	T12317	T12314	conj	Alk6,Alk3
R8008	T12318	T12312	punct	),Alk2
R8009	T12319	T12309	cc	and,type
R801	T1717	T1718	advmod	However,suggested
R8010	T12320	T12309	conj	type,type
R8011	T12321	T12320	nummod	II,type
R8012	T12322	T12323	punct	(,RII
R8013	T12323	T12307	parataxis	RII,receptors
R8014	T12324	T12323	compound	BMP,RII
R8015	T12325	T12323	punct	", ",RII
R8016	T12326	T12323	conj	ActRII,RII
R8017	T12327	T12326	punct	", ",ActRII
R8018	T12328	T12326	cc	and,ActRII
R8019	T12329	T12326	conj	ActRIIb,ActRII
R802	T1719	T1718	punct	", ",suggested
R8020	T12330	T12323	punct	),RII
R8021	T12331	T12332	punct	[,47
R8022	T12332	T12295	parataxis	47,utilize
R8023	T12333	T12332	punct	],47
R8024	T12334	T12295	punct	.,utilize
R8025	T12336	T12337	mark	Once,formed
R8026	T12337	T12345	advcl	formed,direct
R8027	T12338	T12339	det	the,complex
R8028	T12339	T12337	nsubjpass	complex,formed
R8029	T12340	T12339	prep	between,complex
R803	T1720	T1721	det	all,three
R8030	T12341	T12340	pobj	ligand,between
R8031	T12342	T12341	cc	and,ligand
R8032	T12343	T12341	conj	receptors,ligand
R8033	T12344	T12337	auxpass	is,formed
R8034	T12346	T12345	punct	", ",direct
R8035	T12347	T12345	nsubj	BMP2,direct
R8036	T12348	T12347	punct	", ",BMP2
R8037	T12349	T12347	conj	BMP4,BMP2
R8038	T12350	T12349	punct	", ",BMP4
R8039	T12351	T12349	conj	BMP5,BMP4
R804	T1721	T1718	nsubjpass	three,suggested
R8040	T12352	T12351	punct	", ",BMP5
R8041	T12353	T12351	conj	BMP6,BMP5
R8042	T12354	T12353	punct	", ",BMP6
R8043	T12355	T12353	cc	and,BMP6
R8044	T12356	T12353	conj	BMP7,BMP6
R8045	T12357	T12358	det	the,phosphorylation
R8046	T12358	T12345	dobj	phosphorylation,direct
R8047	T12359	T12358	prep	of,phosphorylation
R8048	T12360	T12361	det	the,set
R8049	T12361	T12359	pobj	set,of
R805	T1722	T1721	prep	of,three
R8050	T12362	T12361	amod	same,set
R8051	T12363	T12361	prep	of,set
R8052	T12364	T12365	nmod	BMP,Smads
R8053	T12365	T12363	pobj	Smads,of
R8054	T12366	T12365	nmod	receptor,Smads
R8055	T12367	T12366	punct	–,receptor
R8056	T12368	T12366	amod	specific,receptor
R8057	T12369	T12370	punct	(,1
R8058	T12370	T12358	parataxis	1,phosphorylation
R8059	T12371	T12370	punct	", ",1
R806	T1723	T1724	det	these,molecules
R8060	T12372	T12370	conj	5,1
R8061	T12373	T12372	punct	", ",5
R8062	T12374	T12372	cc	or,5
R8063	T12375	T12372	conj	8,5
R8064	T12376	T12370	punct	),1
R8065	T12377	T12345	punct	", ",direct
R8066	T12378	T12345	cc	and,direct
R8067	T12379	T12380	det	the,signal
R8068	T12380	T12381	nsubj	signal,appears
R8069	T12381	T12345	conj	appears,direct
R807	T1724	T1722	pobj	molecules,of
R8070	T12382	T12383	dep	that,transduced
R8071	T12383	T12380	relcl	transduced,signal
R8072	T12384	T12383	auxpass	is,transduced
R8073	T12385	T12383	agent	by,transduced
R8074	T12386	T12387	det	each,BMP
R8075	T12387	T12385	pobj	BMP,by
R8076	T12388	T12389	aux	to,be
R8077	T12389	T12381	xcomp	be,appears
R8078	T12390	T12389	acomp	identical,be
R8079	T12391	T12389	prep	in,be
R808	T1725	T1721	punct	", ",three
R8080	T12392	T12393	det	the,cells
R8081	T12393	T12391	pobj	cells,in
R8082	T12394	T12393	amod	skeletal,cells
R8083	T12395	T12393	compound	target,cells
R8084	T12396	T12397	punct	[,48
R8085	T12397	T12381	parataxis	48,appears
R8086	T12398	T12399	punct	–,51
R8087	T12399	T12397	prep	51,48
R8088	T12400	T12397	punct	],48
R8089	T12401	T12381	punct	.,appears
R809	T1726	T1721	appos	BMP2,three
R8090	T12403	T12404	nsubjpass	BMPs,shown
R8091	T12405	T12404	aux	have,shown
R8092	T12406	T12404	advmod	also,shown
R8093	T12407	T12404	auxpass	been,shown
R8094	T12408	T12409	aux	to,activate
R8095	T12409	T12404	xcomp	activate,shown
R8096	T12410	T12411	nmod	MAPK,pathways
R8097	T12411	T12409	dobj	pathways,activate
R8098	T12412	T12410	cc	and,MAPK
R8099	T12413	T12410	conj	AKT,MAPK
R81	T242	T215	acomp	difficult,been
R810	T1727	T1726	punct	", ",BMP2
R8100	T12414	T12404	cc	and,shown
R8101	T12415	T12404	punct	", ",shown
R8102	T12416	T12417	prep	based,appears
R8103	T12417	T12404	conj	appears,shown
R8104	T12418	T12416	prep	on,based
R8105	T12419	T12420	amod	current,information
R8106	T12420	T12418	pobj	information,on
R8107	T12421	T12420	amod	published,information
R8108	T12422	T12417	punct	", ",appears
R8109	T12423	T12417	nsubj	each,appears
R811	T1728	T1726	conj	BMP4,BMP2
R8110	T12424	T12423	prep	of,each
R8111	T12425	T12426	det	the,BMPs
R8112	T12426	T12424	pobj	BMPs,of
R8113	T12427	T12426	amod	osteogenic,BMPs
R8114	T12428	T12429	aux	to,have
R8115	T12429	T12417	xcomp	have,appears
R8116	T12430	T12431	det	the,capacity
R8117	T12431	T12429	dobj	capacity,have
R8118	T12432	T12431	amod	same,capacity
R8119	T12433	T12434	aux	to,activate
R812	T1729	T1728	punct	", ",BMP4
R8120	T12434	T12431	acl	activate,capacity
R8121	T12435	T12436	det	these,pathways
R8122	T12436	T12434	dobj	pathways,activate
R8123	T12437	T12436	compound	signaling,pathways
R8124	T12438	T12439	punct	[,52
R8125	T12439	T12417	parataxis	52,appears
R8126	T12440	T12441	punct	–,54
R8127	T12441	T12439	prep	54,52
R8128	T12442	T12439	punct	],52
R8129	T12443	T12417	punct	.,appears
R813	T1730	T1728	cc	and,BMP4
R8130	T12445	T12446	advmod	When,considered
R8131	T12446	T12450	advcl	considered,favor
R8132	T12447	T12448	det	this,information
R8133	T12448	T12446	nsubjpass	information,considered
R8134	T12449	T12446	auxpass	is,considered
R8135	T12451	T12446	prep	along,considered
R8136	T12452	T12451	prep	with,along
R8137	T12453	T12454	poss	our,observation
R8138	T12454	T12452	pobj	observation,with
R8139	T12455	T12456	mark	that,rescue
R814	T1731	T1728	conj	BMP7,BMP4
R8140	T12456	T12454	acl	rescue,observation
R8141	T12457	T12458	nummod	one,allele
R8142	T12458	T12456	nsubj	allele,rescue
R8143	T12459	T12458	prep	of,allele
R8144	T12460	T12461	preconj	either,Bmp2
R8145	T12461	T12459	pobj	Bmp2,of
R8146	T12462	T12461	cc	or,Bmp2
R8147	T12463	T12461	conj	Bmp4,Bmp2
R8148	T12464	T12456	aux	can,rescue
R8149	T12465	T12466	det	the,phenotype
R815	T1732	T1718	punct	", ",suggested
R8150	T12466	T12456	dobj	phenotype,rescue
R8151	T12467	T12466	compound	osteoblast,phenotype
R8152	T12468	T12466	compound	differentiation,phenotype
R8153	T12469	T12466	acl	observed,phenotype
R8154	T12470	T12469	prep	in,observed
R8155	T12471	T12472	nmod	Bmp2C,C
R8156	T12472	T12474	nmod	C,mice
R8157	T12473	T12472	punct	/,C
R8158	T12474	T12470	pobj	mice,in
R8159	T12475	T12472	punct	;,C
R816	T1733	T1718	aux	have,suggested
R8160	T12476	T12477	compound	Bmp4C,C
R8161	T12477	T12472	appos	C,C
R8162	T12478	T12477	punct	/,C
R8163	T12479	T12472	punct	;,C
R8164	T12480	T12481	compound	Prx1,cre
R8165	T12481	T12472	appos	cre,C
R8166	T12482	T12481	punct	:,cre
R8167	T12483	T12481	punct	:,cre
R8168	T12484	T12485	punct	(,see
R8169	T12485	T12446	parataxis	see,considered
R817	T1734	T1718	auxpass	been,suggested
R8170	T12486	T12487	compound	Figures,1I
R8171	T12487	T12485	dobj	1I,see
R8172	T12488	T12487	punct	", ",1I
R8173	T12489	T12487	conj	1J,1I
R8174	T12490	T12489	punct	", ",1J
R8175	T12491	T12489	conj	1Q,1J
R8176	T12492	T12491	punct	", ",1Q
R8177	T12493	T12491	cc	and,1Q
R8178	T12494	T12491	conj	1R,1Q
R8179	T12495	T12494	cc	and,1R
R818	T1735	T1736	aux	to,act
R8180	T12496	T12494	conj	S4,1R
R8181	T12497	T12485	punct	),see
R8182	T12498	T12450	punct	", ",favor
R8183	T12499	T12450	nsubj	we,favor
R8184	T12500	T12501	det	the,hypothesis
R8185	T12501	T12450	dobj	hypothesis,favor
R8186	T12502	T12503	mark	that,requires
R8187	T12503	T12501	acl	requires,hypothesis
R8188	T12504	T12505	compound	bone,formation
R8189	T12505	T12503	nsubj	formation,requires
R819	T1736	T1718	xcomp	act,suggested
R8190	T12506	T12507	det	a,amount
R8191	T12507	T12503	dobj	amount,requires
R8192	T12508	T12507	compound	threshold,amount
R8193	T12509	T12507	prep	of,amount
R8194	T12510	T12511	compound	BMP,signaling
R8195	T12511	T12509	pobj	signaling,of
R8196	T12512	T12513	dep	which,met
R8197	T12513	T12507	relcl	met,amount
R8198	T12514	T12513	auxpass	is,met
R8199	T12515	T12513	neg	not,met
R82	T243	T244	aux	to,decipher
R820	T1737	T1736	prep	in,act
R8200	T12516	T12517	advmod	when,are
R8201	T12517	T12513	advcl	are,met
R8202	T12518	T12519	preconj	both,BMP2
R8203	T12519	T12517	nsubj	BMP2,are
R8204	T12520	T12519	cc	and,BMP2
R8205	T12521	T12519	conj	BMP4,BMP2
R8206	T12522	T12523	advmod	completely,absent
R8207	T12523	T12517	acomp	absent,are
R8208	T12524	T12450	punct	.,favor
R8209	T12526	T12527	nsubj	We,exclude
R821	T1738	T1739	det	a,phase
R8210	T12528	T12527	aux	can,exclude
R8211	T12529	T12527	neg	not,exclude
R8212	T12530	T12527	punct	", ",exclude
R8213	T12531	T12527	advmod	however,exclude
R8214	T12532	T12527	punct	", ",exclude
R8215	T12533	T12534	det	the,possibility
R8216	T12534	T12527	dobj	possibility,exclude
R8217	T12535	T12536	mark	that,have
R8218	T12536	T12534	acl	have,possibility
R8219	T12537	T12536	nsubj	BMP2,have
R822	T1739	T1737	pobj	phase,in
R8220	T12538	T12537	cc	and,BMP2
R8221	T12539	T12537	conj	BMP4,BMP2
R8222	T12540	T12541	amod	distinct,functions
R8223	T12541	T12536	dobj	functions,have
R8224	T12542	T12536	prep	in,have
R8225	T12543	T12542	pobj	osteoblastogenesis,in
R8226	T12544	T12527	punct	.,exclude
R8227	T12546	T12547	poss	Our,analysis
R8228	T12547	T12548	nsubj	analysis,indicates
R8229	T12549	T12547	prep	of,analysis
R823	T1740	T1739	amod	second,phase
R8230	T12550	T12549	pobj	skeletogenesis,of
R8231	T12551	T12552	mark	that,are
R8232	T12552	T12548	ccomp	are,indicates
R8233	T12553	T12552	nsubj	BMP2,are
R8234	T12554	T12553	cc	and,BMP2
R8235	T12555	T12553	conj	BMP4,BMP2
R8236	T12556	T12552	acomp	prerequisite,are
R8237	T12557	T12552	prep	for,are
R8238	T12558	T12557	pobj	osteoblastogenesis,for
R8239	T12559	T12560	mark	while,important
R824	T1741	T1739	amod	distinct,phase
R8240	T12560	T12552	advcl	important,are
R8241	T12561	T12560	advmod	less,important
R8242	T12562	T12560	prep	for,important
R8243	T12563	T12562	pobj	chondrogenesis,for
R8244	T12564	T12548	punct	.,indicates
R8245	T12566	T12567	det	These,studies
R8246	T12567	T12568	nsubj	studies,provide
R8247	T12569	T12570	det	the,evidence
R8248	T12570	T12568	dobj	evidence,provide
R8249	T12571	T12570	amod	first,evidence
R825	T1742	T1739	prep	of,phase
R8250	T12572	T12570	acl	linking,evidence
R8251	T12573	T12572	dobj	BMP2,linking
R8252	T12574	T12573	cc	and,BMP2
R8253	T12575	T12573	conj	BMP4,BMP2
R8254	T12576	T12572	prep	with,linking
R8255	T12577	T12578	compound	bone,formation
R8256	T12578	T12576	pobj	formation,with
R8257	T12579	T12572	prep	in,linking
R8258	T12580	T12581	det	an,setting
R8259	T12581	T12579	pobj	setting,in
R826	T1743	T1744	compound	limb,patterning
R8260	T12582	T12583	advmod	in,vivo
R8261	T12583	T12581	amod	vivo,setting
R8262	T12584	T12585	advmod	where,are
R8263	T12585	T12581	relcl	are,setting
R8264	T12586	T12587	det	the,events
R8265	T12587	T12585	nsubj	events,are
R8266	T12588	T12587	amod	preceding,events
R8267	T12589	T12587	prep	of,events
R8268	T12590	T12589	pobj	chondrogenesis,of
R8269	T12591	T12585	neg	not,are
R827	T1744	T1742	pobj	patterning,of
R8270	T12592	T12585	acomp	compromised,are
R8271	T12593	T12568	punct	.,provide
R8272	T12633	T12632	punct	.,Reagents
R8273	T12635	T12636	nsubjpass	Xylene,purchased
R8274	T12637	T12635	punct	", ",Xylene
R8275	T12638	T12635	conj	formamide,Xylene
R8276	T12639	T12638	punct	", ",formamide
R8277	T12640	T12638	cc	and,formamide
R8278	T12641	T12642	amod	acetic,anhydride
R8279	T12642	T12638	conj	anhydride,formamide
R828	T1745	T1736	punct	", ",act
R8280	T12643	T12636	auxpass	were,purchased
R8281	T12644	T12636	prep	from,purchased
R8282	T12645	T12646	compound	Fisher,Scientific
R8283	T12646	T12644	pobj	Scientific,from
R8284	T12647	T12648	punct	(,http://www.fishersci.com
R8285	T12648	T12646	parataxis	http://www.fishersci.com,Scientific
R8286	T12649	T12648	punct	),http://www.fishersci.com
R8287	T12650	T12636	punct	.,purchased
R8288	T12652	T12653	nmod	Alizarin,S
R8289	T12653	T12655	nsubjpass	S,purchased
R829	T1746	T1747	advmod	when,established
R8290	T12654	T12653	amod	red,S
R8291	T12656	T12653	punct	", ",S
R8292	T12657	T12653	conj	toluidine,S
R8293	T12658	T12657	amod	blue,toluidine
R8294	T12659	T12657	punct	", ",toluidine
R8295	T12660	T12657	conj	paraformaldehyde,toluidine
R8296	T12661	T12660	punct	", ",paraformaldehyde
R8297	T12662	T12660	conj	polyvinylpyrrolidone,paraformaldehyde
R8298	T12663	T12662	punct	", ",polyvinylpyrrolidone
R8299	T12664	T12665	compound	proteinase,K
R83	T244	T215	xcomp	decipher,been
R830	T1747	T1736	advcl	established,act
R8300	T12665	T12662	conj	K,polyvinylpyrrolidone
R8301	T12666	T12665	punct	", ",K
R8302	T12667	T12668	compound	RNase,A
R8303	T12668	T12665	conj	A,K
R8304	T12669	T12668	punct	", ",A
R8305	T12670	T12668	conj	tRNA,A
R8306	T12671	T12670	punct	", ",tRNA
R8307	T12672	T12670	cc	and,tRNA
R8308	T12673	T12670	conj	dithiothreitol,tRNA
R8309	T12674	T12655	auxpass	were,purchased
R831	T1748	T1749	compound	digit,identities
R8310	T12675	T12655	prep	from,purchased
R8311	T12676	T12675	pobj	Sigma,from
R8312	T12677	T12678	punct	(,http://www.sigmaaldrich.com
R8313	T12678	T12676	parataxis	http://www.sigmaaldrich.com,Sigma
R8314	T12679	T12678	punct	),http://www.sigmaaldrich.com
R8315	T12680	T12655	punct	.,purchased
R8316	T12682	T12683	amod	Alcian,8GX
R8317	T12683	T12685	nsubjpass	8GX,purchased
R8318	T12684	T12683	amod	blue,8GX
R8319	T12686	T12685	auxpass	was,purchased
R832	T1749	T1747	nsubjpass	identities,established
R8320	T12687	T12685	prep	from,purchased
R8321	T12688	T12689	compound	Electron,Science
R8322	T12689	T12687	pobj	Science,from
R8323	T12690	T12689	compound	Microscopy,Science
R8324	T12691	T12692	punct	(,http://www.emsdiasum.com
R8325	T12692	T12689	parataxis	http://www.emsdiasum.com,Science
R8326	T12693	T12692	punct	),http://www.emsdiasum.com
R8327	T12694	T12685	punct	.,purchased
R8328	T12696	T12697	compound	Dextran,sulfate
R8329	T12697	T12698	nsubjpass	sulfate,purchased
R833	T1750	T1747	auxpass	are,established
R8330	T12699	T12698	auxpass	was,purchased
R8331	T12700	T12698	prep	from,purchased
R8332	T12701	T12700	pobj	Pharmacia,from
R8333	T12702	T12703	punct	(,http://www.pharmacia.com
R8334	T12703	T12701	parataxis	http://www.pharmacia.com,Pharmacia
R8335	T12704	T12703	punct	),http://www.pharmacia.com
R8336	T12705	T12698	punct	.,purchased
R8337	T12758	T12757	prep	of,Generation
R8338	T12759	T12760	npadvmod	Bmp2,null
R8339	T12760	T12764	amod	null,allele
R834	T1763	T1759	punct	", ",identified
R8340	T12761	T12759	cc	and,Bmp2
R8341	T12762	T12759	conj	Bmp4,Bmp2
R8342	T12763	T12760	amod	conditional,null
R8343	T12764	T12758	pobj	allele,of
R8344	T12765	T12757	punct	.,Generation
R8345	T12767	T12768	nsubj	Mice,were
R8346	T12769	T12767	acl	carrying,Mice
R8347	T12770	T12771	amod	floxed,allele
R8348	T12771	T12769	dobj	allele,carrying
R8349	T12772	T12771	compound	Bmp2,allele
R835	T1751	T1747	advmod	downstream,established
R8350	T12773	T12768	acomp	available,were
R8351	T12774	T12768	prep	through,were
R8352	T12775	T12776	det	a,agreement
R8353	T12776	T12774	pobj	agreement,through
R8354	T12777	T12776	compound	material,agreement
R8355	T12778	T12776	compound	transfer,agreement
R8356	T12779	T12776	prep	between,agreement
R8357	T12780	T12781	compound	Harvard,University
R8358	T12781	T12779	pobj	University,between
R8359	T12782	T12781	cc	and,University
R836	T1764	T1765	det	each,digit
R8360	T12783	T12784	compound	Wyeth,Pharmaceuticals
R8361	T12784	T12781	conj	Pharmaceuticals,University
R8362	T12785	T12768	punct	.,were
R8363	T12787	T12788	prep	In,integrated
R8364	T12789	T12790	det	these,mice
R8365	T12790	T12787	pobj	mice,In
R8366	T12791	T12788	punct	", ",integrated
R8367	T12792	T12793	compound	LoxP,sites
R8368	T12793	T12788	nsubjpass	sites,integrated
R8369	T12794	T12788	auxpass	were,integrated
R837	T1765	T1759	nsubjpass	digit,identified
R8370	T12795	T12796	aux	to,excise
R8371	T12796	T12788	advcl	excise,integrated
R8372	T12797	T12798	det	the,region
R8373	T12798	T12796	dobj	region,excise
R8374	T12799	T12798	amod	entire,region
R8375	T12800	T12801	npadvmod	protein,coding
R8376	T12801	T12798	amod	coding,region
R8377	T12802	T12801	punct	-,coding
R8378	T12803	T12798	prep	in,region
R8379	T12804	T12803	pobj	exon,in
R838	T1752	T1751	prep	of,downstream
R8380	T12805	T12804	nummod	3,exon
R8381	T12806	T12804	prep	of,exon
R8382	T12807	T12808	det	the,gene
R8383	T12808	T12806	pobj	gene,of
R8384	T12809	T12808	compound	Bmp2,gene
R8385	T12810	T12788	punct	.,integrated
R8386	T12812	T12813	nsubjpass	Generation,described
R8387	T12814	T12812	prep	of,Generation
R8388	T12815	T12816	nmod	Bmp4,allele
R8389	T12816	T12814	pobj	allele,of
R839	T1766	T1759	aux	can,identified
R8390	T12817	T12816	amod	conditional,allele
R8391	T12818	T12813	aux	has,described
R8392	T12819	T12813	auxpass	been,described
R8393	T12820	T12821	punct	[,55
R8394	T12821	T12813	parataxis	55,described
R8395	T12822	T12821	nummod	16,55
R8396	T12823	T12821	punct	",",55
R8397	T12824	T12821	punct	],55
R8398	T12825	T12813	punct	.,described
R8399	T12827	T12828	det	The,transgene
R84	T245	T246	det	the,roles
R840	T1767	T1759	auxpass	be,identified
R8400	T12828	T12833	nsubjpass	transgene,described
R8401	T12829	T12830	compound	Prx1,cre
R8402	T12830	T12828	compound	cre,transgene
R8403	T12831	T12830	punct	:,cre
R8404	T12832	T12830	punct	:,cre
R8405	T12834	T12833	aux	has,described
R8406	T12835	T12833	auxpass	been,described
R8407	T12836	T12837	punct	[,32
R8408	T12837	T12833	parataxis	32,described
R8409	T12838	T12837	punct	],32
R841	T1753	T1754	amod	earlier,events
R8410	T12839	T12833	punct	.,described
R8411	T13184	T13183	prep	of,Generation
R8412	T13185	T13186	nmod	Bmp2,Bmp4
R8413	T13186	T13188	npadvmod	Bmp4,conditional
R8414	T13187	T13186	punct	", ",Bmp4
R8415	T13188	T13190	amod	conditional,mice
R8416	T13189	T13188	amod	double,conditional
R8417	T13190	T13184	pobj	mice,of
R8418	T13191	T13190	cc	and,mice
R8419	T13192	T13193	npadvmod	Bmp2,conditional
R842	T1768	T1759	advmod	uniquely,identified
R8420	T13193	T13194	amod	conditional,mice
R8421	T13194	T13190	conj	mice,mice
R8422	T13195	T13194	punct	", ",mice
R8423	T13196	T13197	compound	Bmp7,mutant
R8424	T13197	T13194	compound	mutant,mice
R8425	T13198	T13183	punct	.,Generation
R8426	T13200	T13201	compound	Bmp2 C,C
R8427	T13201	T13203	compound	C,animals
R8428	T13202	T13201	punct	/,C
R8429	T13203	T13204	nsubjpass	animals,crossed
R843	T1769	T1759	prep	based,identified
R8430	T13205	T13204	auxpass	were,crossed
R8431	T13206	T13204	prep	with,crossed
R8432	T13207	T13208	compound	Bmp4 C,C
R8433	T13208	T13210	compound	C,animals
R8434	T13209	T13208	punct	/,C
R8435	T13210	T13206	pobj	animals,with
R8436	T13211	T13212	aux	to,generate
R8437	T13212	T13204	advcl	generate,crossed
R8438	T13213	T13214	nmod	Bmp2 C,animals
R8439	T13214	T13212	dobj	animals,generate
R844	T1754	T1752	pobj	events,of
R8440	T13215	T13213	punct	/,Bmp2 C
R8441	T13216	T13213	punct	+,Bmp2 C
R8442	T13217	T13213	punct	;,Bmp2 C
R8443	T13218	T13213	appos	Bmp4 C,Bmp2 C
R8444	T13219	T13218	punct	/,Bmp4 C
R8445	T13220	T13218	punct	+,Bmp4 C
R8446	T13221	T13204	punct	.,crossed
R8447	T13223	T13224	det	These,animals
R8448	T13224	T13225	nsubjpass	animals,crossed
R8449	T13226	T13225	auxpass	were,crossed
R845	T1770	T1769	prep	on,based
R8450	T13227	T13225	prep	with,crossed
R8451	T13228	T13229	nmod	Bmp2 C,C
R8452	T13229	T13231	nmod	C,animals
R8453	T13230	T13229	punct	/,C
R8454	T13231	T13227	pobj	animals,with
R8455	T13232	T13229	cc	or,C
R8456	T13233	T13234	compound	Bmp4 C,C
R8457	T13234	T13229	conj	C,C
R8458	T13235	T13234	punct	/,C
R8459	T13236	T13237	aux	to,generate
R846	T1771	T1772	poss	its,size
R8460	T13237	T13225	advcl	generate,crossed
R8461	T13238	T13239	nmod	Bmp2 C,C
R8462	T13239	T13241	nmod	C,animals
R8463	T13240	T13239	punct	/,C
R8464	T13241	T13237	dobj	animals,generate
R8465	T13242	T13239	punct	;,C
R8466	T13243	T13239	appos	Bmp4 C,C
R8467	T13244	T13243	punct	/,Bmp4 C
R8468	T13245	T13243	punct	+,Bmp4 C
R8469	T13246	T13239	cc	or,C
R847	T1772	T1770	pobj	size,on
R8470	T13247	T13239	conj	Bmp2 C,C
R8471	T13248	T13247	punct	/,Bmp2 C
R8472	T13249	T13247	punct	+,Bmp2 C
R8473	T13250	T13247	punct	;,Bmp2 C
R8474	T13251	T13252	compound	Bmp4 C,C
R8475	T13252	T13247	appos	C,Bmp2 C
R8476	T13253	T13252	punct	/,C
R8477	T13254	T13237	punct	", ",generate
R8478	T13255	T13237	advmod	respectively,generate
R8479	T13256	T13225	punct	.,crossed
R848	T1755	T1754	compound	patterning,events
R8480	T13258	T13259	nmod	Bmp2 C,C
R8481	T13259	T13261	nmod	C,animals
R8482	T13260	T13259	punct	/,C
R8483	T13261	T13274	nsubjpass	animals,crossed
R8484	T13262	T13259	punct	;,C
R8485	T13263	T13259	appos	Bmp4 C,C
R8486	T13264	T13263	punct	/,Bmp4 C
R8487	T13265	T13263	punct	+,Bmp4 C
R8488	T13266	T13259	cc	or,C
R8489	T13267	T13259	conj	Bmp2 C,C
R849	T1773	T1772	punct	", ",size
R8490	T13268	T13267	punct	/,Bmp2 C
R8491	T13269	T13267	punct	+,Bmp2 C
R8492	T13270	T13267	punct	;,Bmp2 C
R8493	T13271	T13272	compound	Bmp4 C,C
R8494	T13272	T13267	appos	C,Bmp2 C
R8495	T13273	T13272	punct	/,C
R8496	T13275	T13274	auxpass	were,crossed
R8497	T13276	T13274	prep	with,crossed
R8498	T13277	T13278	det	each,other
R8499	T13278	T13276	pobj	other,with
R85	T246	T244	dobj	roles,decipher
R850	T1774	T1772	conj	length,size
R8500	T13279	T13280	aux	to,generate
R8501	T13280	T13274	advcl	generate,crossed
R8502	T13281	T13282	nmod	Bmp2 C,C
R8503	T13282	T13284	nmod	C,animals
R8504	T13283	T13282	punct	/,C
R8505	T13284	T13280	dobj	animals,generate
R8506	T13285	T13282	punct	;,C
R8507	T13286	T13287	compound	Bmp4 C,C
R8508	T13287	T13282	appos	C,C
R8509	T13288	T13287	punct	/,C
R851	T1756	T1718	punct	.,suggested
R8510	T13289	T13274	punct	.,crossed
R8511	T13291	T13292	nmod	Bmp2 C,C
R8512	T13292	T13294	nmod	C,females
R8513	T13293	T13292	punct	/,C
R8514	T13294	T13299	nsubjpass	females,crossed
R8515	T13295	T13292	punct	;,C
R8516	T13296	T13297	compound	Bmp4 C,C
R8517	T13297	T13292	appos	C,C
R8518	T13298	T13297	punct	/,C
R8519	T13300	T13299	auxpass	were,crossed
R852	T1775	T1774	punct	", ",length
R8520	T13301	T13299	prep	with,crossed
R8521	T13302	T13301	pobj	males,with
R8522	T13303	T13302	acl	bearing,males
R8523	T13304	T13305	compound	Prx1,cre
R8524	T13305	T13308	compound	cre,transgene
R8525	T13306	T13305	punct	:,cre
R8526	T13307	T13305	punct	:,cre
R8527	T13308	T13303	dobj	transgene,bearing
R8528	T13309	T13310	aux	to,generate
R8529	T13310	T13299	advcl	generate,crossed
R853	T1776	T1774	conj	number,length
R8530	T13311	T13312	nmod	Bmp2C,animals
R8531	T13312	T13310	dobj	animals,generate
R8532	T13313	T13311	punct	/,Bmp2C
R8533	T13314	T13311	punct	+,Bmp2C
R8534	T13315	T13311	punct	;,Bmp2C
R8535	T13316	T13311	appos	Bmp4C,Bmp2C
R8536	T13317	T13316	punct	/,Bmp4C
R8537	T13318	T13316	punct	+,Bmp4C
R8538	T13319	T13311	punct	;,Bmp2C
R8539	T13320	T13321	compound	Prx1,cre
R854	T1758	T1759	prep	In,identified
R8540	T13321	T13311	appos	cre,Bmp2C
R8541	T13322	T13321	punct	:,cre
R8542	T13323	T13321	punct	:,cre
R8543	T13324	T13299	punct	.,crossed
R8544	T13326	T13327	nmod	Bmp2C,males
R8545	T13327	T13339	nsubjpass	males,crossed
R8546	T13328	T13326	punct	/,Bmp2C
R8547	T13329	T13326	punct	+,Bmp2C
R8548	T13330	T13326	punct	;,Bmp2C
R8549	T13331	T13326	appos	Bmp4C,Bmp2C
R855	T1777	T1776	prep	of,number
R8550	T13332	T13331	punct	/,Bmp4C
R8551	T13333	T13331	punct	+,Bmp4C
R8552	T13334	T13326	punct	;,Bmp2C
R8553	T13335	T13336	compound	Prx1,cre
R8554	T13336	T13326	appos	cre,Bmp2C
R8555	T13337	T13336	punct	:,cre
R8556	T13338	T13336	punct	:,cre
R8557	T13340	T13339	auxpass	were,crossed
R8558	T13341	T13339	prep	with,crossed
R8559	T13342	T13343	nmod	Bmp2C,C
R856	T1778	T1777	pobj	phalanges,of
R8560	T13343	T13345	nmod	C,females
R8561	T13344	T13343	punct	/,C
R8562	T13345	T13341	pobj	females,with
R8563	T13346	T13343	punct	;,C
R8564	T13347	T13348	compound	Bmp4C,C
R8565	T13348	T13343	appos	C,C
R8566	T13349	T13348	punct	/,C
R8567	T13350	T13351	aux	to,generate
R8568	T13351	T13339	advcl	generate,crossed
R8569	T13352	T13353	nmod	Bmp2C,C
R857	T1779	T1776	punct	", ",number
R8570	T13353	T13355	nmod	C,animals
R8571	T13354	T13353	punct	/,C
R8572	T13355	T13351	dobj	animals,generate
R8573	T13356	T13353	punct	;,C
R8574	T13357	T13358	compound	Bmp4C,C
R8575	T13358	T13353	appos	C,C
R8576	T13359	T13358	punct	/,C
R8577	T13360	T13353	punct	;,C
R8578	T13361	T13362	compound	Prx1,cre
R8579	T13362	T13353	appos	cre,C
R858	T1780	T1776	cc	and,number
R8580	T13363	T13362	punct	:,cre
R8581	T13364	T13362	punct	:,cre
R8582	T13365	T13339	punct	.,crossed
R8583	T13367	T13368	nmod	Bmp2C,C
R8584	T13368	T13370	nmod	C,mice
R8585	T13369	T13368	punct	/,C
R8586	T13370	T13375	nsubj	mice,are
R8587	T13371	T13368	punct	;,C
R8588	T13372	T13373	compound	Bmp4C,C
R8589	T13373	T13368	appos	C,C
R859	T1760	T1761	det	the,limb
R8590	T13374	T13373	punct	/,C
R8591	T13376	T13370	acl	carrying,mice
R8592	T13377	T13378	amod	floxed,alleles
R8593	T13378	T13376	dobj	alleles,carrying
R8594	T13379	T13378	prep	of,alleles
R8595	T13380	T13381	det	the,genes
R8596	T13381	T13379	pobj	genes,of
R8597	T13382	T13381	compound	Bmp,genes
R8598	T13383	T13381	prep	without,genes
R8599	T13384	T13385	compound	Prx1,cre
R86	T247	T246	amod	specific,roles
R860	T1781	T1776	conj	location,number
R8600	T13385	T13383	pobj	cre,without
R8601	T13386	T13385	punct	:,cre
R8602	T13387	T13385	punct	:,cre
R8603	T13388	T13389	amod	wild,type
R8604	T13389	T13375	attr	type,are
R8605	T13390	T13389	punct	-,type
R8606	T13391	T13389	punct	(,type
R8607	T13392	T13389	appos	WT,type
R8608	T13393	T13375	punct	),are
R8609	T13394	T13375	punct	.,are
R861	T1782	T1781	prep	within,location
R8610	T13396	T13397	nsubjpass	Genotyping,done
R8611	T13398	T13396	prep	of,Genotyping
R8612	T13399	T13398	pobj	animals,of
R8613	T13400	T13397	auxpass	were,done
R8614	T13401	T13397	prep	by,done
R8615	T13402	T13403	compound	PCR,analyses
R8616	T13403	T13401	pobj	analyses,by
R8617	T13404	T13403	prep	with,analyses
R8618	T13405	T13406	det	the,GTGTGGTCCACCGCATCAC
R8619	T13406	T13404	pobj	GTGTGGTCCACCGCATCAC,with
R862	T1761	T1758	pobj	limb,In
R8620	T13407	T13406	nmod	primers,GTGTGGTCCACCGCATCAC
R8621	T13408	T13409	punct	(,5
R8622	T13409	T13406	parataxis	5,GTGTGGTCCACCGCATCAC
R8623	T13410	T13409	punct	′,5
R8624	T13411	T13412	punct	–,3
R8625	T13412	T13409	prep	3,5
R8626	T13413	T13412	punct	′,3
R8627	T13414	T13409	punct	),5
R8628	T13415	T13416	punct	(,AHP2
R8629	T13416	T13406	parataxis	AHP2,GTGTGGTCCACCGCATCAC
R863	T1783	T1784	det	the,autopod
R8630	T13417	T13418	punct	–,9
R8631	T13418	T13416	prep	9,AHP2
R8632	T13419	T13416	punct	),AHP2
R8633	T13420	T13406	cc	and,GTGTGGTCCACCGCATCAC
R8634	T13421	T13406	conj	GGCAGACATTGTATCTCTAGG,GTGTGGTCCACCGCATCAC
R8635	T13422	T13423	punct	(,AHP2
R8636	T13423	T13421	parataxis	AHP2,GGCAGACATTGTATCTCTAGG
R8637	T13424	T13425	punct	–,35
R8638	T13425	T13423	prep	35,AHP2
R8639	T13426	T13423	punct	),AHP2
R864	T1784	T1782	pobj	autopod,within
R8640	T13427	T13406	prep	for,GTGTGGTCCACCGCATCAC
R8641	T13428	T13427	pobj	Bmp2,for
R8642	T13429	T13428	cc	and,Bmp2
R8643	T13430	T13428	conj	AGACTCTTTAGTGAGCATTTTCAAC,Bmp2
R8644	T13431	T13432	punct	(,order
R8645	T13432	T13430	parataxis	order,AGACTCTTTAGTGAGCATTTTCAAC
R8646	T13433	T13432	dep	No.,order
R8647	T13434	T13433	nummod	79,No.
R8648	T13435	T13432	punct	[,order
R8649	T13436	T13432	prep	of,order
R865	T1785	T1759	punct	.,identified
R8650	T13437	T13436	pobj	primers,of
R8651	T13438	T13437	acl	tried,primers
R8652	T13439	T13432	punct	],order
R8653	T13440	T13432	punct	),order
R8654	T13441	T13406	cc	and,GTGTGGTCCACCGCATCAC
R8655	T13442	T13406	conj	AGCCCAATTTCCACAACTTC,GTGTGGTCCACCGCATCAC
R8656	T13443	T13444	punct	(,No.
R8657	T13444	T13442	parataxis	No.,AGCCCAATTTCCACAACTTC
R8658	T13445	T13444	nummod	80,No.
R8659	T13446	T13444	punct	),No.
R866	T1762	T1761	compound	vertebrate,limb
R8660	T13447	T13442	prep	for,AGCCCAATTTCCACAACTTC
R8661	T13448	T13447	pobj	Bmp4,for
R8662	T13449	T13403	prep	following,analyses
R8663	T13450	T13449	pobj	extraction,following
R8664	T13451	T13450	prep	of,extraction
R8665	T13452	T13453	amod	genomic,DNA
R8666	T13453	T13451	pobj	DNA,of
R8667	T13454	T13450	prep	from,extraction
R8668	T13455	T13456	amod	embryonic,tail
R8669	T13456	T13454	pobj	tail,from
R867	T1787	T1788	prep	As,becomes
R8670	T13457	T13455	cc	or,embryonic
R8671	T13458	T13455	conj	adult,embryonic
R8672	T13459	T13397	punct	.,done
R8673	T13461	T13462	prep	For,amplifies
R8674	T13463	T13461	pobj	Bmp2,For
R8675	T13464	T13462	punct	", ",amplifies
R8676	T13465	T13466	det	the,allele
R8677	T13466	T13462	nsubj	allele,amplifies
R8678	T13467	T13466	amod	floxed,allele
R8679	T13468	T13462	prep	as,amplifies
R868	T1869	T1849	conj	suggested,expressed
R8680	T13469	T13470	det	a,product
R8681	T13470	T13468	pobj	product,as
R8682	T13471	T13472	nummod	545,bp
R8683	T13472	T13470	compound	bp,product
R8684	T13473	T13472	punct	-,bp
R8685	T13474	T13462	punct	", ",amplifies
R8686	T13475	T13476	mark	while,amplifies
R8687	T13476	T13462	advcl	amplifies,amplifies
R8688	T13477	T13478	det	the,allele
R8689	T13478	T13476	nsubj	allele,amplifies
R869	T1789	T1790	det	a,consequence
R8690	T13479	T13480	amod	wild,type
R8691	T13480	T13478	compound	type,allele
R8692	T13481	T13480	punct	-,type
R8693	T13482	T13476	prep	as,amplifies
R8694	T13483	T13484	det	a,product
R8695	T13484	T13482	pobj	product,as
R8696	T13485	T13486	nummod	474,bp
R8697	T13486	T13484	compound	bp,product
R8698	T13487	T13486	punct	-,bp
R8699	T13488	T13462	punct	.,amplifies
R87	T248	T246	prep	of,roles
R870	T1790	T1787	pobj	consequence,As
R8700	T13490	T13491	prep	For,amplifies
R8701	T13492	T13490	pobj	Bmp4,For
R8702	T13493	T13491	punct	", ",amplifies
R8703	T13494	T13495	det	the,allele
R8704	T13495	T13491	nsubj	allele,amplifies
R8705	T13496	T13495	amod	floxed,allele
R8706	T13497	T13491	prep	as,amplifies
R8707	T13498	T13499	det	a,product
R8708	T13499	T13497	pobj	product,as
R8709	T13500	T13501	nummod	220,bp
R871	T1870	T1868	acl	inhibiting,experiments
R8710	T13501	T13499	compound	bp,product
R8711	T13502	T13501	punct	-,bp
R8712	T13503	T13491	punct	", ",amplifies
R8713	T13504	T13505	mark	while,amplifies
R8714	T13505	T13491	advcl	amplifies,amplifies
R8715	T13506	T13507	det	the,allele
R8716	T13507	T13505	nsubj	allele,amplifies
R8717	T13508	T13509	amod	wild,type
R8718	T13509	T13507	compound	type,allele
R8719	T13510	T13509	punct	-,type
R872	T1791	T1790	prep	of,consequence
R8720	T13511	T13505	prep	as,amplifies
R8721	T13512	T13513	det	a,product
R8722	T13513	T13511	pobj	product,as
R8723	T13514	T13515	nummod	180,bp
R8724	T13515	T13513	compound	bp,product
R8725	T13516	T13515	punct	-,bp
R8726	T13517	T13491	punct	.,amplifies
R8727	T13519	T13520	nsubjpass	Protocols,approved
R8728	T13521	T13519	acl	utilized,Protocols
R8729	T13522	T13521	prep	for,utilized
R873	T1792	T1793	det	the,establishment
R8730	T13523	T13524	compound	mouse,experiments
R8731	T13524	T13522	pobj	experiments,for
R8732	T13525	T13520	auxpass	were,approved
R8733	T13526	T13520	agent	by,approved
R8734	T13527	T13528	det	the,Committee
R8735	T13528	T13526	pobj	Committee,by
R8736	T13529	T13528	nmod	Harvard,Committee
R8737	T13530	T13528	nmod	University,Committee
R8738	T13531	T13528	nmod	Institutional,Committee
R8739	T13532	T13528	nmod	Animal,Committee
R874	T1871	T1872	compound	BMP,signaling
R8740	T13533	T13528	nmod	Care,Committee
R8741	T13534	T13533	cc	and,Care
R8742	T13535	T13533	conj	Use,Care
R8743	T13536	T13537	punct	(,Tabin
R8744	T13537	T13528	parataxis	Tabin,Committee
R8745	T13538	T13537	compound	Cliff,Tabin
R8746	T13539	T13537	punct	#,Tabin
R8747	T13540	T13537	nummod	02735,Tabin
R8748	T13541	T13537	punct	),Tabin
R8749	T13542	T13520	punct	.,approved
R875	T1793	T1791	pobj	establishment,of
R8750	T13544	T13545	det	The,strategy
R8751	T13545	T13546	nsubj	strategy,is
R8752	T13547	T13545	prep	for,strategy
R8753	T13548	T13547	pcomp	generating,for
R8754	T13549	T13550	det	a,null
R8755	T13550	T13548	dobj	null,generating
R8756	T13551	T13552	npadvmod	Bmp2,conditional
R8757	T13552	T13550	amod	conditional,null
R8758	T13553	T13548	prep	in,generating
R8759	T13554	T13555	det	the,background
R876	T1794	T1793	amod	initial,establishment
R8760	T13555	T13553	pobj	background,in
R8761	T13556	T13555	nmod	BMP7,background
R8762	T13557	T13556	punct	−,BMP7
R8763	T13558	T13556	punct	/,BMP7
R8764	T13559	T13556	punct	−,BMP7
R8765	T13560	T13546	acomp	similar,is
R8766	T13561	T13560	prep	to,similar
R8767	T13562	T13563	det	the,strategy
R8768	T13563	T13561	pobj	strategy,to
R8769	T13564	T13563	acl	used,strategy
R877	T1872	T1870	dobj	signaling,inhibiting
R8770	T13565	T13564	prep	for,used
R8771	T13566	T13565	pcomp	generating,for
R8772	T13567	T13568	det	the,allele
R8773	T13568	T13566	dobj	allele,generating
R8774	T13569	T13570	nmod	Bmp2,Bmp4
R8775	T13570	T13572	npadvmod	Bmp4,conditional
R8776	T13571	T13570	punct	", ",Bmp4
R8777	T13572	T13568	amod	conditional,allele
R8778	T13573	T13572	amod	double,conditional
R8779	T13574	T13546	prep	with,is
R878	T1795	T1793	prep	of,establishment
R8780	T13575	T13576	nummod	three,differences
R8781	T13576	T13574	pobj	differences,with
R8782	T13577	T13576	amod	important,differences
R8783	T13578	T13546	punct	.,is
R8784	T13580	T13581	advmod	First,is
R8785	T13582	T13581	punct	", ",is
R8786	T13583	T13584	det	the,Bmp7
R8787	T13584	T13581	nsubj	Bmp7,is
R8788	T13585	T13584	punct	−,Bmp7
R8789	T13586	T13584	punct	/,Bmp7
R879	T1796	T1797	amod	anterior,posterior
R8790	T13587	T13584	punct	−,Bmp7
R8791	T13588	T13589	punct	(,strain
R8792	T13589	T13584	parataxis	strain,Bmp7
R8793	T13590	T13591	advmod	kindly,donated
R8794	T13591	T13589	acl	donated,strain
R8795	T13592	T13591	agent	by,donated
R8796	T13593	T13594	compound	Dr.,Robertson
R8797	T13594	T13592	pobj	Robertson,by
R8798	T13595	T13594	compound	Liz,Robertson
R8799	T13596	T13597	punct	[,12
R88	T249	T250	det	these,molecules
R880	T1797	T1799	amod	posterior,positioned
R8800	T13597	T13591	parataxis	12,donated
R8801	T13598	T13597	punct	],12
R8802	T13599	T13589	punct	),strain
R8803	T13600	T13601	cc	not,allele
R8804	T13601	T13581	attr	allele,is
R8805	T13602	T13601	det	a,allele
R8806	T13603	T13601	amod	conditional,allele
R8807	T13604	T13601	cc	but,allele
R8808	T13605	T13606	det	a,mutation
R8809	T13606	T13601	conj	mutation,allele
R881	T1798	T1797	punct	-,posterior
R8810	T13607	T13606	amod	null,mutation
R8811	T13608	T13581	punct	.,is
R8812	T13610	T13611	advmod	Second,survive
R8813	T13612	T13611	punct	", ",survive
R8814	T13613	T13614	nmod	Bmp7,mice
R8815	T13614	T13611	nsubj	mice,survive
R8816	T13615	T13613	punct	−,Bmp7
R8817	T13616	T13613	punct	/,Bmp7
R8818	T13617	T13613	punct	−,Bmp7
R8819	T13618	T13611	aux	do,survive
R882	T1799	T1800	amod	positioned,information
R8820	T13619	T13611	neg	not,survive
R8821	T13620	T13611	prep	past,survive
R8822	T13621	T13620	pobj	birth,past
R8823	T13622	T13611	prep	due,survive
R8824	T13623	T13622	pcomp	to,due
R8825	T13624	T13625	compound	kidney,failure
R8826	T13625	T13622	pobj	failure,due
R8827	T13626	T13627	punct	[,12
R8828	T13627	T13611	parataxis	12,survive
R8829	T13628	T13627	punct	],12
R883	T1800	T1795	pobj	information,of
R8830	T13629	T13611	cc	and,survive
R8831	T13630	T13631	advmod	hence,maintained
R8832	T13631	T13611	conj	maintained,survive
R8833	T13632	T13631	aux	can,maintained
R8834	T13633	T13631	neg	not,maintained
R8835	T13634	T13631	auxpass	be,maintained
R8836	T13635	T13631	prep	as,maintained
R8837	T13636	T13635	amod	homozygous,as
R8838	T13637	T13631	prep	for,maintained
R8839	T13638	T13639	det	the,allele
R884	T1873	T1870	prep	in,inhibiting
R8840	T13639	T13637	pobj	allele,for
R8841	T13640	T13639	amod	null,allele
R8842	T13641	T13611	punct	.,survive
R8843	T13643	T13644	advmod	Third,are
R8844	T13645	T13644	punct	", ",are
R8845	T13646	T13644	nsubj	Bmp2,are
R8846	T13647	T13646	cc	and,Bmp2
R8847	T13648	T13646	conj	Bmp7,Bmp2
R8848	T13649	T13644	prep	on,are
R8849	T13650	T13651	det	the,chromosome
R885	T1801	T1793	prep	within,establishment
R8850	T13651	T13649	pobj	chromosome,on
R8851	T13652	T13651	amod	same,chromosome
R8852	T13653	T13651	punct	(,chromosome
R8853	T13654	T13651	appos	Chromosome,chromosome
R8854	T13655	T13654	nummod	2,Chromosome
R8855	T13656	T13651	punct	),chromosome
R8856	T13657	T13644	punct	.,are
R8857	T13659	T13660	aux	To,obtain
R8858	T13660	T13661	advcl	obtain,crossed
R8859	T13662	T13663	compound	Bmp2C,C
R886	T1802	T1803	det	the,limb
R8860	T13663	T13660	dobj	C,obtain
R8861	T13664	T13663	punct	/,C
R8862	T13665	T13663	punct	", ",C
R8863	T13666	T13663	appos	Bmp7,C
R8864	T13667	T13666	punct	−,Bmp7
R8865	T13668	T13666	punct	/,Bmp7
R8866	T13669	T13666	punct	−,Bmp7
R8867	T13670	T13663	punct	;,C
R8868	T13671	T13672	compound	Prx1,cre
R8869	T13672	T13663	appos	cre,C
R887	T1803	T1801	pobj	limb,within
R8870	T13673	T13672	punct	:,cre
R8871	T13674	T13672	punct	:,cre
R8872	T13675	T13661	nsubj	we,crossed
R8873	T13676	T13677	nmod	Bmp2C,C
R8874	T13677	T13679	nmod	C,females
R8875	T13678	T13677	punct	/,C
R8876	T13679	T13661	dobj	females,crossed
R8877	T13680	T13677	punct	", ",C
R8878	T13681	T13677	appos	Bmp7,C
R8879	T13682	T13681	punct	−,Bmp7
R888	T1874	T1875	det	this,tissue
R8880	T13683	T13681	punct	/,Bmp7
R8881	T13684	T13681	punct	+,Bmp7
R8882	T13685	T13661	prep	with,crossed
R8883	T13686	T13687	nmod	Bmp2C,males
R8884	T13687	T13685	pobj	males,with
R8885	T13688	T13686	punct	/,Bmp2C
R8886	T13689	T13686	punct	+,Bmp2C
R8887	T13690	T13686	punct	", ",Bmp2C
R8888	T13691	T13686	appos	Bmp7,Bmp2C
R8889	T13692	T13691	punct	−,Bmp7
R889	T1804	T1793	prep	by,establishment
R8890	T13693	T13691	punct	/,Bmp7
R8891	T13694	T13691	punct	+,Bmp7
R8892	T13695	T13686	punct	;,Bmp2C
R8893	T13696	T13697	compound	Prx1,cre
R8894	T13697	T13686	appos	cre,Bmp2C
R8895	T13698	T13697	punct	:,cre
R8896	T13699	T13697	punct	:,cre
R8897	T13700	T13661	punct	.,crossed
R8898	T13702	T13703	nmod	Bmp2C,C
R8899	T13703	T13705	nmod	C,progeny
R89	T250	T248	pobj	molecules,of
R890	T1875	T1873	pobj	tissue,in
R8900	T13704	T13703	punct	/,C
R8901	T13705	T13716	nsubjpass	progeny,obtained
R8902	T13706	T13703	punct	", ",C
R8903	T13707	T13703	appos	Bmp7,C
R8904	T13708	T13707	punct	−,Bmp7
R8905	T13709	T13707	punct	/,Bmp7
R8906	T13710	T13707	punct	−,Bmp7
R8907	T13711	T13703	punct	;,C
R8908	T13712	T13713	compound	Prx1,cre
R8909	T13713	T13703	appos	cre,C
R891	T1805	T1806	nmod	SHH,signaling
R8910	T13714	T13713	punct	:,cre
R8911	T13715	T13713	punct	:,cre
R8912	T13717	T13716	auxpass	were,obtained
R8913	T13718	T13716	prep	at,obtained
R8914	T13719	T13720	det	a,rate
R8915	T13720	T13718	pobj	rate,at
R8916	T13721	T13722	advmod	slightly,higher
R8917	T13722	T13720	amod	higher,rate
R8918	T13723	T13722	prep	than,higher
R8919	T13724	T13723	pobj	1,than
R892	T1876	T1877	mark	that,be
R8920	T13725	T13724	prep	in,1
R8921	T13726	T13725	pobj	16,in
R8922	T13727	T13716	punct	.,obtained
R8923	T13729	T13730	prep	For,used
R8924	T13731	T13729	pcomp	genotyping,For
R8925	T13732	T13731	prep	of,genotyping
R8926	T13733	T13734	compound	Bmp7,locus
R8927	T13734	T13732	pobj	locus,of
R8928	T13735	T13730	nsubj	we,used
R8929	T13736	T13737	det	the,set
R893	T1806	T1804	pobj	signaling,by
R8930	T13737	T13730	dep	set,used
R8931	T13738	T13737	amod	following,set
R8932	T13739	T13737	prep	of,set
R8933	T13740	T13739	pobj	primers,of
R8934	T13741	T13737	punct	: ,set
R8935	T13742	T13743	nmod	Bmp7_ExI_5,TCGCCTGCAGCAAGTGACCTCGGGTC
R8936	T13743	T13737	conj	TCGCCTGCAGCAAGTGACCTCGGGTC,set
R8937	T13744	T13742	punct	′,Bmp7_ExI_5
R8938	T13745	T13746	punct	(,5
R8939	T13746	T13742	parataxis	5,Bmp7_ExI_5
R894	T1877	T1869	ccomp	be,suggested
R8940	T13747	T13746	punct	′,5
R8941	T13748	T13749	punct	–,3
R8942	T13749	T13746	prep	3,5
R8943	T13750	T13749	punct	′,3
R8944	T13751	T13746	punct	),5
R8945	T13752	T13743	punct	;,TCGCCTGCAGCAAGTGACCTCGGGTC
R8946	T13753	T13754	nmod	Bmp7_ExI_3,TAGGGGTAGGAGAAGCCCTGTCCGTCC
R8947	T13754	T13743	conj	TAGGGGTAGGAGAAGCCCTGTCCGTCC,TCGCCTGCAGCAAGTGACCTCGGGTC
R8948	T13755	T13753	punct	′,Bmp7_ExI_3
R8949	T13756	T13757	punct	(,5
R895	T1807	T1805	cc	and,SHH
R8950	T13757	T13753	parataxis	5,Bmp7_ExI_3
R8951	T13758	T13757	punct	′,5
R8952	T13759	T13760	punct	–,3
R8953	T13760	T13757	prep	3,5
R8954	T13761	T13760	punct	′,3
R8955	T13762	T13757	punct	),5
R8956	T13763	T13754	cc	and,TAGGGGTAGGAGAAGCCCTGTCCGTCC
R8957	T13764	T13765	nmod	Beta,5
R8958	T13765	T13768	nummod	5,CTGCATACGCTTGATCCGGCTACCTGC
R8959	T13766	T13765	punct	-,5
R896	T1878	T1879	amod	differential,levels
R8960	T13767	T13765	nmod	geo,5
R8961	T13768	T13754	conj	CTGCATACGCTTGATCCGGCTACCTGC,TAGGGGTAGGAGAAGCCCTGTCCGTCC
R8962	T13769	T13765	punct	′,5
R8963	T13770	T13765	prep	of,5
R8964	T13771	T13770	pobj	3,of
R8965	T13772	T13771	punct	′,3
R8966	T13773	T13774	punct	(,5
R8967	T13774	T13765	parataxis	5,5
R8968	T13775	T13774	punct	′,5
R8969	T13776	T13777	punct	–,3
R897	T1808	T1807	punct	/,and
R8970	T13777	T13774	prep	3,5
R8971	T13778	T13777	punct	′,3
R8972	T13779	T13774	punct	),5
R8973	T13780	T13730	punct	.,used
R8974	T13782	T13783	prep	From,amplifies
R8975	T13784	T13785	det	a,chromosome
R8976	T13785	T13782	pobj	chromosome,From
R8977	T13786	T13787	amod	wild,type
R8978	T13787	T13785	compound	type,chromosome
R8979	T13788	T13787	punct	-,type
R898	T1809	T1807	cc	or,and
R8980	T13789	T13783	nsubj	Bmp7_ExI_5,amplifies
R8981	T13790	T13789	punct	′,Bmp7_ExI_5
R8982	T13791	T13789	cc	and,Bmp7_ExI_5
R8983	T13792	T13789	conj	Bmp7_ExI_3,Bmp7_ExI_5
R8984	T13793	T13792	punct	′,Bmp7_ExI_3
R8985	T13794	T13795	det	an,product
R8986	T13795	T13783	dobj	product,amplifies
R8987	T13796	T13797	advmod	approximately,423
R8988	T13797	T13798	nummod	423,bp
R8989	T13798	T13795	compound	bp,product
R899	T1879	T1877	nsubj	levels,be
R8990	T13799	T13798	punct	-,bp
R8991	T13800	T13795	compound	PCR,product
R8992	T13801	T13783	punct	", ",amplifies
R8993	T13802	T13803	mark	while,fails
R8994	T13803	T13783	advcl	fails,amplifies
R8995	T13804	T13803	prep	from,fails
R8996	T13805	T13806	det	the,mutant
R8997	T13806	T13804	pobj	mutant,from
R8998	T13807	T13806	compound	lacZ,mutant
R8999	T13808	T13806	compound	insertion,mutant
R9	T163	T161	cc	and,BMP4
R90	T251	T250	compound	BMP,molecules
R900	T1810	T1805	conj	BMP,SHH
R9000	T13809	T13810	det	this,pair
R9001	T13810	T13803	nsubj	pair,fails
R9002	T13811	T13812	aux	to,amplify
R9003	T13812	T13803	xcomp	amplify,fails
R9004	T13813	T13814	det	any,product
R9005	T13814	T13812	dobj	product,amplify
R9006	T13815	T13783	punct	.,amplifies
R9007	T13817	T13818	nsubj	Bmp7_ExI_5,amplifies
R9008	T13819	T13817	punct	′,Bmp7_ExI_5
R9009	T13820	T13817	cc	and,Bmp7_ExI_5
R901	T1811	T1788	punct	", ",becomes
R9010	T13821	T13822	nmod	Beta,5
R9011	T13822	T13817	conj	5,Bmp7_ExI_5
R9012	T13823	T13822	punct	-,5
R9013	T13824	T13822	nmod	geo,5
R9014	T13825	T13822	punct	′,5
R9015	T13826	T13822	prep	of,5
R9016	T13827	T13826	pobj	3,of
R9017	T13828	T13827	punct	′,3
R9018	T13829	T13830	punct	(,designed
R9019	T13830	T13822	parataxis	designed,5
R902	T1880	T1879	prep	of,levels
R9020	T13831	T13832	det	this,primer
R9021	T13832	T13830	nsubjpass	primer,designed
R9022	T13833	T13830	auxpass	is,designed
R9023	T13834	T13830	prep	from,designed
R9024	T13835	T13836	det	the,cassette
R9025	T13836	T13834	pobj	cassette,from
R9026	T13837	T13838	compound	Beta,geo
R9027	T13838	T13836	compound	geo,cassette
R9028	T13839	T13838	punct	-,geo
R9029	T13840	T13830	punct	),designed
R903	T1812	T1813	det	the,mesenchyme
R9030	T13841	T13842	det	an,product
R9031	T13842	T13818	dobj	product,amplifies
R9032	T13843	T13844	advmod	approximately,1
R9033	T13844	T13845	nummod	1,kb
R9034	T13845	T13842	compound	kb,product
R9035	T13846	T13845	punct	-,kb
R9036	T13847	T13842	compound	PCR,product
R9037	T13848	T13842	prep	from,product
R9038	T13849	T13850	det	the,chromosome
R9039	T13850	T13848	pobj	chromosome,from
R904	T1813	T1788	nsubj	mesenchyme,becomes
R9040	T13851	T13852	npadvmod	lacZ,inserted
R9041	T13852	T13850	amod	inserted,chromosome
R9042	T13853	T13818	punct	.,amplifies
R9043	T13914	T13915	amod	Skeletal,analysis
R9044	T13916	T13915	punct	.,analysis
R9045	T13918	T13919	poss	Our,protocol
R9046	T13919	T13920	nsubj	protocol,is
R9047	T13921	T13922	det	an,adaptation
R9048	T13922	T13920	attr	adaptation,is
R9049	T13923	T13922	prep	of,adaptation
R905	T1881	T1880	pobj	BMPs,of
R9050	T13924	T13925	det	the,procedure
R9051	T13925	T13923	pobj	procedure,of
R9052	T13926	T13925	acl	described,procedure
R9053	T13927	T13928	punct	[,56
R9054	T13928	T13926	parataxis	56,described
R9055	T13929	T13928	punct	],56
R9056	T13930	T13926	advmod	previously,described
R9057	T13931	T13920	punct	.,is
R9058	T13933	T13934	amod	Newborn,animals
R9059	T13934	T13937	nsubjpass	animals,killed
R906	T1814	T1813	amod	interdigital,mesenchyme
R9060	T13935	T13933	cc	and,Newborn
R9061	T13936	T13933	conj	older,Newborn
R9062	T13938	T13937	auxpass	were,killed
R9063	T13939	T13937	advcl	using,killed
R9064	T13940	T13941	compound	carbon,dioxide
R9065	T13941	T13939	dobj	dioxide,using
R9066	T13942	T13937	punct	.,killed
R9067	T13944	T13945	nmod	Skin,tissues
R9068	T13945	T13951	nsubjpass	tissues,removed
R9069	T13946	T13944	punct	", ",Skin
R907	T1815	T1813	prep	of,mesenchyme
R9070	T13947	T13944	conj	viscera,Skin
R9071	T13948	T13947	punct	", ",viscera
R9072	T13949	T13947	cc	and,viscera
R9073	T13950	T13947	conj	adipose,viscera
R9074	T13952	T13951	auxpass	were,removed
R9075	T13953	T13951	prep	within,removed
R9076	T13954	T13955	nummod	2,h
R9077	T13955	T13953	pobj	h,within
R9078	T13956	T13955	prep	after,h
R9079	T13957	T13956	pobj	death,after
R908	T1816	T1817	det	the,plate
R9080	T13958	T13951	punct	", ",removed
R9081	T13959	T13951	cc	and,removed
R9082	T13960	T13961	nsubjpass	samples,fixed
R9083	T13961	T13951	conj	fixed,removed
R9084	T13962	T13961	auxpass	were,fixed
R9085	T13963	T13961	prep	in,fixed
R9086	T13964	T13965	nummod	95,%
R9087	T13965	T13966	compound	%,ethanol
R9088	T13966	T13963	pobj	ethanol,in
R9089	T13967	T13961	prep	for,fixed
R909	T1882	T1879	prep	in,levels
R9090	T13968	T13969	nummod	5,d
R9091	T13969	T13967	pobj	d,for
R9092	T13970	T13961	punct	.,fixed
R9093	T13972	T13973	nsubjpass	Samples,placed
R9094	T13974	T13973	auxpass	were,placed
R9095	T13975	T13973	advmod	then,placed
R9096	T13976	T13973	prep	in,placed
R9097	T13977	T13976	pobj	acetone,in
R9098	T13978	T13979	aux	to,remove
R9099	T13979	T13973	advcl	remove,placed
R91	T252	T246	prep	during,roles
R910	T1817	T1815	pobj	plate,of
R9100	T13980	T13981	amod	residual,fat
R9101	T13981	T13979	dobj	fat,remove
R9102	T13982	T13979	prep	for,remove
R9103	T13983	T13984	nummod	2,d
R9104	T13984	T13982	pobj	d,for
R9105	T13985	T13973	punct	.,placed
R9106	T13987	T13988	prep	For,removed
R9107	T13989	T13990	compound	E13.5,embryos
R9108	T13990	T13987	pobj	embryos,For
R9109	T13991	T13988	punct	", ",removed
R911	T1818	T1817	compound	hand,plate
R9110	T13992	T13988	nsubjpass	skin,removed
R9111	T13993	T13988	auxpass	was,removed
R9112	T13994	T13988	neg	not,removed
R9113	T13995	T13988	punct	.,removed
R9114	T13997	T13998	det	The,animals
R9115	T13998	T14000	nsubjpass	animals,stained
R9116	T13999	T13998	amod	dehydrated,animals
R9117	T14001	T14000	auxpass	were,stained
R9118	T14002	T14000	prep	with,stained
R9119	T14003	T14004	nummod	0.015,%
R912	T1883	T1884	det	the,mesenchyme
R9120	T14004	T14005	nmod	%,v
R9121	T14005	T14002	pobj	v,with
R9122	T14006	T14005	nmod	w,v
R9123	T14007	T14005	punct	/,v
R9124	T14008	T14005	amod	Alcian,v
R9125	T14009	T14005	amod	blue,v
R9126	T14010	T14005	cc	and,v
R9127	T14011	T14012	nummod	0.005,%
R9128	T14012	T14013	nmod	%,v
R9129	T14013	T14005	conj	v,v
R913	T1819	T1788	acomp	specified,becomes
R9130	T14014	T14013	nmod	w,v
R9131	T14015	T14013	punct	/,v
R9132	T14016	T14013	amod	Alizarin,v
R9133	T14017	T14013	amod	red,v
R9134	T14018	T14000	prep	in,stained
R9135	T14019	T14020	det	a,mixture
R9136	T14020	T14018	pobj	mixture,in
R9137	T14021	T14020	nummod	1,mixture
R9138	T14022	T14023	punct	:,19
R9139	T14023	T14021	prep	19,1
R914	T1820	T1788	prep	in,becomes
R9140	T14024	T14020	prep	of,mixture
R9141	T14025	T14026	amod	glacial,acid
R9142	T14026	T14024	pobj	acid,of
R9143	T14027	T14026	amod	acetic,acid
R9144	T14028	T14026	cc	and,acid
R9145	T14029	T14030	nummod	70,%
R9146	T14030	T14031	compound	%,ethanol
R9147	T14031	T14026	conj	ethanol,acid
R9148	T14032	T14000	punct	.,stained
R9149	T14034	T14035	det	The,animals
R915	T1821	T1822	det	a,manner
R9150	T14035	T14037	nsubj	animals,stained
R9151	T14036	T14035	amod	younger,animals
R9152	T14038	T14037	aux	were,stained
R9153	T14039	T14037	prep	for,stained
R9154	T14040	T14041	nummod	5,h
R9155	T14041	T14039	pobj	h,for
R9156	T14042	T14037	prep	at,stained
R9157	T14043	T14044	nummod	37,°C
R9158	T14044	T14042	pobj	°C,at
R9159	T14045	T14037	cc	and,stained
R916	T1884	T1882	pobj	mesenchyme,in
R9160	T14046	T14047	advmod	then,at
R9161	T14047	T14037	conj	at,stained
R9162	T14048	T14047	advmod	overnight,at
R9163	T14049	T14050	compound	room,temperature
R9164	T14050	T14047	pobj	temperature,at
R9165	T14051	T14037	punct	", ",stained
R9166	T14052	T14053	mark	while,stained
R9167	T14053	T14037	advcl	stained,stained
R9168	T14054	T14055	det	the,newborns
R9169	T14055	T14053	nsubjpass	newborns,stained
R917	T1822	T1820	pobj	manner,in
R9170	T14056	T14053	auxpass	were,stained
R9171	T14057	T14053	advmod	overnight,stained
R9172	T14058	T14053	prep	at,stained
R9173	T14059	T14060	nummod	37,°C
R9174	T14060	T14058	pobj	°C,at
R9175	T14061	T14053	advcl	followed,stained
R9176	T14062	T14061	agent	by,followed
R9177	T14063	T14064	nummod	2,d
R9178	T14064	T14062	pobj	d,by
R9179	T14065	T14064	prep	at,d
R918	T1823	T1822	amod	graded,manner
R9180	T14066	T14067	compound	room,temperature
R9181	T14067	T14065	pobj	temperature,at
R9182	T14068	T14037	punct	.,stained
R9183	T14070	T14071	nmod	The,skeletons
R9184	T14071	T14073	nsubjpass	skeletons,kept
R9185	T14072	T14071	amod	stained,skeletons
R9186	T14074	T14073	auxpass	were,kept
R9187	T14075	T14073	prep	in,kept
R9188	T14076	T14077	nummod	1,%
R9189	T14077	T14078	compound	%,KOH
R919	T1885	T1884	amod	interdigital,mesenchyme
R9190	T14078	T14075	pobj	KOH,in
R9191	T14079	T14073	punct	", ",kept
R9192	T14080	T14073	prep	with,kept
R9193	T14081	T14082	amod	occasional,change
R9194	T14082	T14080	pobj	change,with
R9195	T14083	T14082	prep	of,change
R9196	T14084	T14083	pobj	solution,of
R9197	T14085	T14073	punct	", ",kept
R9198	T14086	T14087	mark	until,cleared
R9199	T14087	T14073	advcl	cleared,kept
R92	T253	T254	amod	different,stages
R920	T1824	T1788	punct	.,becomes
R9200	T14088	T14073	punct	.,kept
R9201	T14090	T14091	det	The,skeletons
R9202	T14091	T14093	nsubjpass	skeletons,transferred
R9203	T14092	T14091	amod	cleared,skeletons
R9204	T14094	T14093	auxpass	were,transferred
R9205	T14095	T14093	prep	into,transferred
R9206	T14096	T14097	nummod	100,%
R9207	T14097	T14098	compound	%,glycerol
R9208	T14098	T14095	pobj	glycerol,into
R9209	T14099	T14093	cc	and,transferred
R921	T1826	T1827	nmod	Grafting,experiments
R9210	T14100	T14093	conj	photographed,transferred
R9211	T14101	T14093	punct	.,transferred
R9212	T14125	T14126	nmod	Acridine,staining
R9213	T14127	T14125	amod	orange,Acridine
R9214	T14128	T14126	prep	for,staining
R9215	T14129	T14130	amod	apoptotic,cells
R9216	T14130	T14128	pobj	cells,for
R9217	T14131	T14126	punct	.,staining
R9218	T14133	T14134	det	A,stock
R9219	T14134	T14136	nsubjpass	stock,diluted
R922	T1886	T1877	aux	might,be
R9220	T14135	T14134	amod	working,stock
R9221	T14137	T14134	prep	of,stock
R9222	T14138	T14139	nummod	5,mg
R9223	T14139	T14140	nmod	mg,orange
R9224	T14140	T14137	pobj	orange,of
R9225	T14141	T14142	punct	/,ml
R9226	T14142	T14139	prep	ml,mg
R9227	T14143	T14140	compound	acridine,orange
R9228	T14144	T14136	auxpass	was,diluted
R9229	T14145	T14136	npadvmod	1,diluted
R923	T1827	T1830	nsubj	experiments,shown
R9230	T14146	T14147	punct	:,"10,000"
R9231	T14147	T14145	prep	"10,000",1
R9232	T14148	T14136	prep	in,diluted
R9233	T14149	T14148	pobj	PBS,in
R9234	T14150	T14136	punct	.,diluted
R9235	T14152	T14153	amod	Dissected,embryos
R9236	T14153	T14155	nsubjpass	embryos,transferred
R9237	T14154	T14153	amod	fresh,embryos
R9238	T14156	T14155	auxpass	were,transferred
R9239	T14157	T14155	prep	to,transferred
R924	T1828	T1826	cc	and,Grafting
R9240	T14158	T14159	det	the,solution
R9241	T14159	T14157	pobj	solution,to
R9242	T14160	T14159	amod	working,solution
R9243	T14161	T14159	prep	of,solution
R9244	T14162	T14163	compound	acridine,orange
R9245	T14163	T14161	pobj	orange,of
R9246	T14164	T14155	cc	and,transferred
R9247	T14165	T14155	conj	incubated,transferred
R9248	T14166	T14165	prep	for,incubated
R9249	T14167	T14168	nummod	30,min
R925	T1887	T1888	det	the,factor
R9250	T14168	T14166	pobj	min,for
R9251	T14169	T14165	prep	at,incubated
R9252	T14170	T14171	nummod	37,°C
R9253	T14171	T14169	pobj	°C,at
R9254	T14172	T14165	prep	in,incubated
R9255	T14173	T14174	det	the,dark
R9256	T14174	T14172	pobj	dark,in
R9257	T14175	T14155	punct	.,transferred
R9258	T14177	T14178	nsubjpass	Embryos,washed
R9259	T14179	T14178	auxpass	were,washed
R926	T1829	T1826	conj	extirpation,Grafting
R9260	T14180	T14178	advmod	then,washed
R9261	T14181	T14178	advmod	twice,washed
R9262	T14182	T14178	prep	in,washed
R9263	T14183	T14182	pobj	PBS,in
R9264	T14184	T14178	prep	for,washed
R9265	T14185	T14186	nummod	5,min
R9266	T14186	T14184	pobj	min,for
R9267	T14187	T14178	cc	and,washed
R9268	T14188	T14178	conj	viewed,washed
R9269	T14189	T14188	prep	with,viewed
R927	T1831	T1830	aux	have,shown
R9270	T14190	T14191	det	a,microscope
R9271	T14191	T14189	pobj	microscope,with
R9272	T14192	T14191	compound	fluorescence,microscope
R9273	T14193	T14178	punct	.,washed
R9274	T14208	T14209	amod	Whole,mount
R9275	T14209	T14210	nmod	mount,hybridization
R9276	T14211	T14212	advmod	in,situ
R9277	T14212	T14210	amod	situ,hybridization
R9278	T14213	T14210	punct	.,hybridization
R9279	T14215	T14216	advmod	In,situ
R928	T1888	T1877	attr	factor,be
R9280	T14216	T14217	amod	situ,hybridization
R9281	T14217	T14218	compound	hybridization,staining
R9282	T14218	T14219	nsubjpass	staining,performed
R9283	T14220	T14218	prep	on,staining
R9284	T14221	T14222	amod	whole,embryos
R9285	T14222	T14220	pobj	embryos,on
R9286	T14223	T14219	auxpass	was,performed
R9287	T14224	T14225	mark	as,described
R9288	T14225	T14219	advcl	described,performed
R9289	T14226	T14227	punct	[,57
R929	T1832	T1833	mark	that,is
R9290	T14227	T14219	parataxis	57,performed
R9291	T14228	T14227	punct	],57
R9292	T14229	T14219	punct	.,performed
R9293	T14231	T14232	prep	For,see
R9294	T14233	T14234	nmod	section,hybridizations
R9295	T14234	T14231	pobj	hybridizations,For
R9296	T14235	T14236	advmod	in,situ
R9297	T14236	T14234	amod	situ,hybridizations
R9298	T14237	T14232	punct	", ",see
R9299	T14238	T14232	intj	please,see
R93	T254	T252	pobj	stages,during
R930	T1833	T1830	ccomp	is,shown
R9300	T14239	T14232	advmod	below,see
R9301	T14240	T14232	punct	.,see
R9302	T14446	T14445	punct	", ",Histology
R9303	T14447	T14448	nmod	section,hybridizations
R9304	T14448	T14445	conj	hybridizations,Histology
R9305	T14449	T14450	advmod	in,situ
R9306	T14450	T14448	amod	situ,hybridizations
R9307	T14451	T14448	punct	", ",hybridizations
R9308	T14452	T14448	cc	and,hybridizations
R9309	T14453	T14448	conj	immunohistochemistry,hybridizations
R931	T1834	T1833	nsubj	it,is
R9310	T14454	T14453	punct	.,immunohistochemistry
R9311	T14456	T14457	prep	For,fixed
R9312	T14458	T14456	pobj	histology,For
R9313	T14459	T14458	cc	and,histology
R9314	T14460	T14461	nmod	section,hybridization
R9315	T14461	T14458	conj	hybridization,histology
R9316	T14462	T14463	advmod	in,situ
R9317	T14463	T14461	amod	situ,hybridization
R9318	T14464	T14457	punct	", ",fixed
R9319	T14465	T14457	nsubjpass	samples,fixed
R932	T1889	T1888	amod	relevant,factor
R9320	T14466	T14457	auxpass	were,fixed
R9321	T14467	T14457	prep	in,fixed
R9322	T14468	T14469	nummod	4,%
R9323	T14469	T14470	compound	%,paraformaldehyde
R9324	T14470	T14467	pobj	paraformaldehyde,in
R9325	T14471	T14457	prep	in,fixed
R9326	T14472	T14471	pobj	PBS,in
R9327	T14473	T14457	prep	at,fixed
R9328	T14474	T14475	nummod	4,°C
R9329	T14475	T14473	pobj	°C,at
R933	T1835	T1836	det	this,tissue
R9330	T14476	T14457	advmod	overnight,fixed
R9331	T14477	T14457	punct	.,fixed
R9332	T14479	T14480	amod	Newborn,animals
R9333	T14480	T14483	nsubjpass	animals,decalcified
R9334	T14481	T14479	cc	and,Newborn
R9335	T14482	T14479	conj	older,Newborn
R9336	T14484	T14483	auxpass	were,decalcified
R9337	T14485	T14483	advmod	then,decalcified
R9338	T14486	T14483	prep	in,decalcified
R9339	T14487	T14488	compound	Tris,buffer
R934	T1836	T1833	attr	tissue,is
R9340	T14488	T14486	pobj	buffer,in
R9341	T14489	T14488	acl	containing,buffer
R9342	T14490	T14491	nummod	10,%
R9343	T14491	T14492	compound	%,EDTA
R9344	T14492	T14489	dobj	EDTA,containing
R9345	T14493	T14492	cc	and,EDTA
R9346	T14494	T14495	nummod	7.5,%
R9347	T14495	T14496	compound	%,polyvinylpyrrolidone
R9348	T14496	T14492	conj	polyvinylpyrrolidone,EDTA
R9349	T14497	T14498	punct	(,pH
R935	T1837	T1836	amod	polarized,tissue
R9350	T14498	T14496	parataxis	pH,polyvinylpyrrolidone
R9351	T14499	T14498	nummod	7.5,pH
R9352	T14500	T14498	punct	),pH
R9353	T14501	T14483	prep	at,decalcified
R9354	T14502	T14503	nummod	4,°C
R9355	T14503	T14501	pobj	°C,at
R9356	T14504	T14483	prep	for,decalcified
R9357	T14505	T14506	nummod	3,wks
R9358	T14506	T14504	pobj	wks,for
R9359	T14507	T14483	punct	.,decalcified
R936	T1838	T1836	amod	interdigital,tissue
R9360	T14509	T14510	nsubjpass	Samples,dehydrated
R9361	T14511	T14510	auxpass	were,dehydrated
R9362	T14512	T14510	advmod	then,dehydrated
R9363	T14513	T14510	prep	through,dehydrated
R9364	T14514	T14515	det	a,series
R9365	T14515	T14513	pobj	series,through
R9366	T14516	T14515	amod	graded,series
R9367	T14517	T14515	compound	ethanol,series
R9368	T14518	T14510	punct	", ",dehydrated
R9369	T14519	T14510	conj	cleared,dehydrated
R937	T1890	T1888	acl	directing,factor
R9370	T14520	T14519	prep	in,cleared
R9371	T14521	T14520	pobj	xylene,in
R9372	T14522	T14519	punct	", ",cleared
R9373	T14523	T14519	cc	and,cleared
R9374	T14524	T14519	conj	embedded,cleared
R9375	T14525	T14524	prep	in,embedded
R9376	T14526	T14525	pobj	paraffin,in
R9377	T14527	T14510	punct	.,dehydrated
R9378	T14529	T14530	nummod	8,μm
R9379	T14530	T14531	compound	μm,sections
R938	T1839	T1840	dep	which,directs
R9380	T14531	T14532	nsubj	sections,collected
R9381	T14533	T14532	aux	were,collected
R9382	T14534	T14532	cc	and,collected
R9383	T14535	T14532	conj	stained,collected
R9384	T14536	T14535	prep	with,stained
R9385	T14537	T14536	pobj	hematoxylin,with
R9386	T14538	T14537	cc	and,hematoxylin
R9387	T14539	T14537	conj	eosin,hematoxylin
R9388	T14540	T14537	punct	(,hematoxylin
R9389	T14541	T14537	appos	H,hematoxylin
R939	T1840	T1833	ccomp	directs,is
R9390	T14542	T14541	cc	&,H
R9391	T14543	T14541	conj	E,H
R9392	T14544	T14537	punct	),hematoxylin
R9393	T14545	T14537	cc	or,hematoxylin
R9394	T14546	T14537	conj	toluidine,hematoxylin
R9395	T14547	T14546	amod	blue,toluidine
R9396	T14548	T14532	advcl	following,collected
R9397	T14549	T14550	amod	standard,procedure
R9398	T14550	T14548	dobj	procedure,following
R9399	T14551	T14532	punct	.,collected
R94	T255	T254	prep	of,stages
R940	T1841	T1842	compound	digit,morphology
R9400	T14553	T14554	nmod	Section,hybridization
R9401	T14554	T14557	nsubjpass	hybridization,performed
R9402	T14555	T14556	advmod	in,situ
R9403	T14556	T14554	amod	situ,hybridization
R9404	T14558	T14554	prep	with,hybridization
R9405	T14559	T14560	npadvmod	digoxigenin,labeled
R9406	T14560	T14561	amod	labeled,probes
R9407	T14561	T14558	pobj	probes,with
R9408	T14562	T14557	auxpass	was,performed
R9409	T14563	T14564	mark	as,described
R941	T1842	T1840	dobj	morphology,directs
R9410	T14564	T14557	advcl	described,performed
R9411	T14565	T14566	punct	[,57
R9412	T14566	T14557	parataxis	57,performed
R9413	T14567	T14566	punct	],57
R9414	T14568	T14557	punct	.,performed
R9415	T14570	T14571	nmod	Section,hybridization
R9416	T14571	T14574	nsubjpass	hybridization,performed
R9417	T14572	T14573	advmod	in,situ
R9418	T14573	T14571	amod	situ,hybridization
R9419	T14575	T14571	prep	with,hybridization
R942	T1843	T1844	punct	[,15
R9420	T14576	T14577	amod	radiolabeled,probes
R9421	T14577	T14575	pobj	probes,with
R9422	T14578	T14574	auxpass	was,performed
R9423	T14579	T14580	mark	as,described
R9424	T14580	T14574	advcl	described,performed
R9425	T14581	T14582	punct	[,58
R9426	T14582	T14580	parataxis	58,described
R9427	T14583	T14582	punct	],58
R9428	T14584	T14574	prep	with,performed
R9429	T14585	T14586	det	a,modification
R943	T1844	T1833	parataxis	15,is
R9430	T14586	T14584	pobj	modification,with
R9431	T14587	T14586	amod	small,modification
R9432	T14588	T14574	punct	.,performed
R9433	T14590	T14591	advmod	Briefly,deparaffinized
R9434	T14592	T14591	punct	", ",deparaffinized
R9435	T14593	T14591	nsubjpass	sections,deparaffinized
R9436	T14594	T14591	auxpass	were,deparaffinized
R9437	T14595	T14591	prep	in,deparaffinized
R9438	T14596	T14595	pobj	xylene,in
R9439	T14597	T14591	cc	and,deparaffinized
R944	T1891	T1892	compound	digit,morphology
R9440	T14598	T14591	conj	rehydrated,deparaffinized
R9441	T14599	T14598	prep	through,rehydrated
R9442	T14600	T14601	det	a,series
R9443	T14601	T14599	pobj	series,through
R9444	T14602	T14601	amod	graded,series
R9445	T14603	T14601	compound	ethanol,series
R9446	T14604	T14591	punct	.,deparaffinized
R9447	T14606	T14607	nsubjpass	Sections,postfixed
R9448	T14608	T14607	auxpass	were,postfixed
R9449	T14609	T14607	prep	in,postfixed
R945	T1845	T1844	punct	],15
R9450	T14610	T14611	nummod	4,%
R9451	T14611	T14612	compound	%,paraformaldehyde
R9452	T14612	T14609	pobj	paraformaldehyde,in
R9453	T14613	T14607	prep	in,postfixed
R9454	T14614	T14613	pobj	PBS,in
R9455	T14615	T14607	prep	at,postfixed
R9456	T14616	T14617	compound	room,temperature
R9457	T14617	T14615	pobj	temperature,at
R9458	T14618	T14607	prep	for,postfixed
R9459	T14619	T14620	nummod	15,min
R946	T1846	T1830	punct	.,shown
R9460	T14620	T14618	pobj	min,for
R9461	T14621	T14607	punct	", ",postfixed
R9462	T14622	T14607	conj	digested,postfixed
R9463	T14623	T14622	prep	with,digested
R9464	T14624	T14625	nummod	10,μg
R9465	T14625	T14626	nmod	μg,K
R9466	T14626	T14623	pobj	K,with
R9467	T14627	T14628	punct	/,ml
R9468	T14628	T14625	prep	ml,μg
R9469	T14629	T14626	compound	proteinase,K
R947	T1892	T1890	dobj	morphology,directing
R9470	T14630	T14622	prep	at,digested
R9471	T14631	T14632	compound	room,temperature
R9472	T14632	T14630	pobj	temperature,at
R9473	T14633	T14622	prep	for,digested
R9474	T14634	T14635	nummod	10,min
R9475	T14635	T14633	pobj	min,for
R9476	T14636	T14622	punct	", ",digested
R9477	T14637	T14622	cc	and,digested
R9478	T14638	T14639	advmod	again,fixed
R9479	T14639	T14622	conj	fixed,digested
R948	T1848	T1849	nsubjpass	BMP2,expressed
R9480	T14640	T14639	prep	in,fixed
R9481	T14641	T14642	nummod	4,%
R9482	T14642	T14643	compound	%,paraformaldehyde
R9483	T14643	T14640	pobj	paraformaldehyde,in
R9484	T14644	T14639	prep	at,fixed
R9485	T14645	T14646	compound	room,temperature
R9486	T14646	T14644	pobj	temperature,at
R9487	T14647	T14639	prep	for,fixed
R9488	T14648	T14649	nummod	10,min
R9489	T14649	T14647	pobj	min,for
R949	T1893	T1894	punct	[,15
R9490	T14650	T14639	cc	and,fixed
R9491	T14651	T14639	conj	acetylated,fixed
R9492	T14652	T14651	prep	in,acetylated
R9493	T14653	T14654	det	the,solution
R9494	T14654	T14652	pobj	solution,in
R9495	T14655	T14654	acl	containing,solution
R9496	T14656	T14657	nummod	0.2,%
R9497	T14657	T14658	nmod	%,acid
R9498	T14658	T14655	dobj	acid,containing
R9499	T14659	T14658	amod	hydrochloric,acid
R95	T256	T257	compound	limb,development
R950	T1850	T1848	punct	", ",BMP2
R9500	T14660	T14658	punct	", ",acid
R9501	T14661	T14662	nummod	0.1,M
R9502	T14662	T14663	compound	M,amine
R9503	T14663	T14658	conj	amine,acid
R9504	T14664	T14663	compound	triethanol,amine
R9505	T14665	T14663	punct	", ",amine
R9506	T14666	T14663	cc	and,amine
R9507	T14667	T14668	nummod	0.25,%
R9508	T14668	T14669	nmod	%,anhydride
R9509	T14669	T14663	conj	anhydride,amine
R951	T1851	T1848	conj	BMP4,BMP2
R9510	T14670	T14669	amod	acetic,anhydride
R9511	T14671	T14655	prep	at,containing
R9512	T14672	T14673	compound	room,temperature
R9513	T14673	T14671	pobj	temperature,at
R9514	T14674	T14655	prep	for,containing
R9515	T14675	T14676	nummod	10,min
R9516	T14676	T14674	pobj	min,for
R9517	T14677	T14607	punct	.,postfixed
R9518	T14679	T14680	nsubjpass	Sections,dehydrated
R9519	T14681	T14680	auxpass	were,dehydrated
R952	T1894	T1869	parataxis	15,suggested
R9520	T14682	T14680	advmod	then,dehydrated
R9521	T14683	T14680	prep	in,dehydrated
R9522	T14684	T14685	amod	increasing,concentrations
R9523	T14685	T14683	pobj	concentrations,in
R9524	T14686	T14685	prep	of,concentrations
R9525	T14687	T14686	pobj	ethanol,of
R9526	T14688	T14680	cc	and,dehydrated
R9527	T14689	T14690	dep	air,dried
R9528	T14690	T14680	conj	dried,dehydrated
R9529	T14691	T14690	punct	-,dried
R953	T1852	T1851	punct	", ",BMP4
R9530	T14692	T14680	punct	.,dehydrated
R9531	T14694	T14695	nsubjpass	Hybridization,performed
R9532	T14696	T14695	auxpass	was,performed
R9533	T14697	T14695	prep	in,performed
R9534	T14698	T14699	det	a,chamber
R9535	T14699	T14697	pobj	chamber,in
R9536	T14700	T14699	amod	humidified,chamber
R9537	T14701	T14695	prep	in,performed
R9538	T14702	T14703	det	a,solution
R9539	T14703	T14701	pobj	solution,in
R954	T1853	T1851	cc	and,BMP4
R9540	T14704	T14703	acl	containing,solution
R9541	T14705	T14706	nummod	50,%
R9542	T14706	T14707	compound	%,formamide
R9543	T14707	T14704	dobj	formamide,containing
R9544	T14708	T14707	punct	", ",formamide
R9545	T14709	T14710	nummod	10,%
R9546	T14710	T14711	compound	%,sulfate
R9547	T14711	T14707	conj	sulfate,formamide
R9548	T14712	T14711	compound	dextran,sulfate
R9549	T14713	T14711	punct	", ",sulfate
R955	T1854	T1851	conj	BMP7,BMP4
R9550	T14714	T14715	nummod	1,solution
R9551	T14715	T14711	conj	solution,sulfate
R9552	T14716	T14714	punct	×,1
R9553	T14717	T14715	poss	Denhardt,solution
R9554	T14718	T14717	case	's,Denhardt
R9555	T14719	T14715	punct	", ",solution
R9556	T14720	T14721	nummod	0.6,M
R9557	T14721	T14722	compound	M,chloride
R9558	T14722	T14715	conj	chloride,solution
R9559	T14723	T14722	compound	sodium,chloride
R956	T1895	T1894	punct	],15
R9560	T14724	T14722	punct	", ",chloride
R9561	T14725	T14726	nummod	0.01,M
R9562	T14726	T14727	compound	M,buffer
R9563	T14727	T14722	conj	buffer,chloride
R9564	T14728	T14727	compound	Tris,buffer
R9565	T14729	T14730	punct	(,pH
R9566	T14730	T14727	parataxis	pH,buffer
R9567	T14731	T14730	nummod	7.5,pH
R9568	T14732	T14730	punct	),pH
R9569	T14733	T14727	punct	", ",buffer
R957	T1855	T1849	auxpass	are,expressed
R9570	T14734	T14735	nummod	0.001,M
R9571	T14735	T14736	compound	M,EDTA
R9572	T14736	T14727	conj	EDTA,buffer
R9573	T14737	T14736	punct	", ",EDTA
R9574	T14738	T14739	nummod	0.05,M
R9575	T14739	T14740	compound	M,dithiothreitol
R9576	T14740	T14736	conj	dithiothreitol,EDTA
R9577	T14741	T14740	punct	", ",dithiothreitol
R9578	T14742	T14743	nummod	0.25,%
R9579	T14743	T14744	compound	%,SDS
R958	T1856	T1849	advmod	all,expressed
R9580	T14744	T14740	conj	SDS,dithiothreitol
R9581	T14745	T14744	punct	", ",SDS
R9582	T14746	T14747	nummod	200,μg
R9583	T14747	T14748	compound	μg,tRNA
R9584	T14748	T14744	conj	tRNA,SDS
R9585	T14749	T14748	punct	", ",tRNA
R9586	T14750	T14748	cc	and,tRNA
R9587	T14751	T14752	nmod	35S,probe
R9588	T14752	T14748	conj	probe,tRNA
R9589	T14753	T14751	punct	-,35S
R959	T1896	T1869	punct	.,suggested
R9590	T14754	T14751	amod	labeled,35S
R9591	T14755	T14752	compound	cRNA,probe
R9592	T14756	T14752	prep	at,probe
R9593	T14757	T14758	det	the,concentration
R9594	T14758	T14756	pobj	concentration,at
R9595	T14759	T14758	amod	final,concentration
R9596	T14760	T14758	prep	of,concentration
R9597	T14761	T14762	quantmod	5,106
R9598	T14762	T14764	nummod	106,cpm
R9599	T14763	T14762	punct	×,106
R96	T257	T255	pobj	development,of
R960	T1857	T1849	prep	in,expressed
R9600	T14764	T14760	pobj	cpm,of
R9601	T14765	T14766	punct	/,ml
R9602	T14766	T14764	prep	ml,cpm
R9603	T14767	T14695	prep	at,performed
R9604	T14768	T14769	nummod	55,°C
R9605	T14769	T14767	pobj	°C,at
R9606	T14770	T14695	prep	for,performed
R9607	T14771	T14772	nummod	16,h
R9608	T14772	T14770	pobj	h,for
R9609	T14773	T14695	punct	.,performed
R961	T1858	T1859	det	the,mesenchyme
R9610	T14775	T14776	prep	After,washed
R9611	T14777	T14775	pobj	hybridization,After
R9612	T14778	T14776	punct	", ",washed
R9613	T14779	T14776	nsubjpass	sections,washed
R9614	T14780	T14776	auxpass	were,washed
R9615	T14781	T14776	prep	with,washed
R9616	T14782	T14783	det	a,solution
R9617	T14783	T14781	pobj	solution,with
R9618	T14784	T14783	acl	containing,solution
R9619	T14785	T14786	nummod	5,SSC
R962	T1859	T1857	pobj	mesenchyme,in
R9620	T14786	T14784	dobj	SSC,containing
R9621	T14787	T14785	punct	×,5
R9622	T14788	T14786	cc	and,SSC
R9623	T14789	T14790	nummod	10,mM
R9624	T14790	T14791	compound	mM,dithiothreitol
R9625	T14791	T14786	conj	dithiothreitol,SSC
R9626	T14792	T14776	prep	at,washed
R9627	T14793	T14794	nummod	50,°C
R9628	T14794	T14792	pobj	°C,at
R9629	T14795	T14776	prep	for,washed
R963	T1898	T1899	mark	As,noted
R9630	T14796	T14797	nummod	30,min
R9631	T14797	T14795	pobj	min,for
R9632	T14798	T14776	punct	", ",washed
R9633	T14799	T14776	conj	incubated,washed
R9634	T14800	T14799	prep	in,incubated
R9635	T14801	T14802	det	a,solution
R9636	T14802	T14800	pobj	solution,in
R9637	T14803	T14802	acl	containing,solution
R9638	T14804	T14805	nummod	50,%
R9639	T14805	T14806	compound	%,formamide
R964	T1860	T1859	amod	interdigital,mesenchyme
R9640	T14806	T14803	dobj	formamide,containing
R9641	T14807	T14806	punct	", ",formamide
R9642	T14808	T14809	nummod	2,SSC
R9643	T14809	T14806	conj	SSC,formamide
R9644	T14810	T14808	punct	×,2
R9645	T14811	T14809	punct	", ",SSC
R9646	T14812	T14809	cc	and,SSC
R9647	T14813	T14814	nummod	10,mM
R9648	T14814	T14815	compound	mM,dithiothreitol
R9649	T14815	T14809	conj	dithiothreitol,SSC
R965	T1861	T1862	punct	[,14
R9650	T14816	T14799	prep	at,incubated
R9651	T14817	T14818	nummod	65,°C
R9652	T14818	T14816	pobj	°C,at
R9653	T14819	T14799	prep	for,incubated
R9654	T14820	T14821	nummod	30,min
R9655	T14821	T14819	pobj	min,for
R9656	T14822	T14799	punct	", ",incubated
R9657	T14823	T14799	conj	treated,incubated
R9658	T14824	T14823	prep	in,treated
R9659	T14825	T14826	det	a,solution
R966	T1862	T1849	parataxis	14,expressed
R9660	T14826	T14824	pobj	solution,in
R9661	T14827	T14826	acl	containing,solution
R9662	T14828	T14829	nummod	10,μg
R9663	T14829	T14830	nmod	μg,A
R9664	T14830	T14827	dobj	A,containing
R9665	T14831	T14832	punct	/,ml
R9666	T14832	T14829	prep	ml,μg
R9667	T14833	T14830	compound	RNase,A
R9668	T14834	T14827	prep	in,containing
R9669	T14835	T14834	pobj	TNE,in
R967	T1863	T1862	nummod	4,14
R9670	T14836	T14837	punct	[,buffer
R9671	T14837	T14835	parataxis	buffer,TNE
R9672	T14838	T14839	nummod	0.01,M
R9673	T14839	T14837	compound	M,buffer
R9674	T14840	T14837	compound	Tris,buffer
R9675	T14841	T14842	punct	(,pH
R9676	T14842	T14837	parataxis	pH,buffer
R9677	T14843	T14842	nummod	7.6,pH
R9678	T14844	T14842	punct	),pH
R9679	T14845	T14837	punct	", ",buffer
R968	T1899	T1900	advcl	noted,produce
R9680	T14846	T14847	nummod	0.5,M
R9681	T14847	T14848	compound	M,chloride
R9682	T14848	T14837	conj	chloride,buffer
R9683	T14849	T14848	compound	sodium,chloride
R9684	T14850	T14848	punct	", ",chloride
R9685	T14851	T14848	cc	and,chloride
R9686	T14852	T14853	nummod	0.001,M
R9687	T14853	T14854	compound	M,EDTA
R9688	T14854	T14848	conj	EDTA,chloride
R9689	T14855	T14837	punct	],buffer
R969	T1864	T1862	punct	",",14
R9690	T14856	T14823	prep	at,treated
R9691	T14857	T14858	nummod	37,°C
R9692	T14858	T14856	pobj	°C,at
R9693	T14859	T14823	prep	for,treated
R9694	T14860	T14861	nummod	30,min
R9695	T14861	T14859	pobj	min,for
R9696	T14862	T14823	cc	and,treated
R9697	T14863	T14864	advmod	then,washed
R9698	T14864	T14823	conj	washed,treated
R9699	T14865	T14864	prep	with,washed
R97	T258	T215	punct	.,been
R970	T1865	T1862	punct	],14
R9700	T14866	T14867	nummod	50,%
R9701	T14867	T14868	compound	%,formamide
R9702	T14868	T14865	pobj	formamide,with
R9703	T14869	T14868	punct	", ",formamide
R9704	T14870	T14871	nummod	2,SSC
R9705	T14871	T14868	conj	SSC,formamide
R9706	T14872	T14870	punct	×,2
R9707	T14873	T14871	punct	", ",SSC
R9708	T14874	T14871	cc	and,SSC
R9709	T14875	T14876	nummod	10,mM
R971	T1901	T1899	advmod	above,noted
R9710	T14876	T14877	compound	mM,dithiothreitol
R9711	T14877	T14871	conj	dithiothreitol,SSC
R9712	T14878	T14864	prep	at,washed
R9713	T14879	T14880	nummod	65,°C
R9714	T14880	T14878	pobj	°C,at
R9715	T14881	T14864	prep	for,washed
R9716	T14882	T14883	det	an,min
R9717	T14883	T14881	pobj	min,for
R9718	T14884	T14883	amod	additional,min
R9719	T14885	T14883	nummod	30,min
R972	T1866	T1849	punct	", ",expressed
R9720	T14886	T14864	advcl	followed,washed
R9721	T14887	T14886	agent	by,followed
R9722	T14888	T14889	nummod	two,washes
R9723	T14889	T14887	pobj	washes,by
R9724	T14890	T14889	prep	with,washes
R9725	T14891	T14892	nummod	2,SSC
R9726	T14892	T14890	pobj	SSC,with
R9727	T14893	T14891	punct	×,2
R9728	T14894	T14895	punct	/,dithiothreitol
R9729	T14895	T14892	prep	dithiothreitol,SSC
R973	T1867	T1849	cc	and,expressed
R9730	T14896	T14897	nummod	10,mM
R9731	T14897	T14895	compound	mM,dithiothreitol
R9732	T14898	T14889	prep	at,washes
R9733	T14899	T14900	nummod	65,°C
R9734	T14900	T14898	pobj	°C,at
R9735	T14901	T14889	prep	for,washes
R9736	T14902	T14903	nummod	30,min
R9737	T14903	T14901	pobj	min,for
R9738	T14904	T14889	punct	", ",washes
R9739	T14905	T14889	cc	and,washes
R974	T1868	T1869	nsubj	experiments,suggested
R9740	T14906	T14907	nummod	0.1,SSC
R9741	T14907	T14889	conj	SSC,washes
R9742	T14908	T14906	punct	×,0.1
R9743	T14909	T14910	punct	/,dithiothreitol
R9744	T14910	T14907	prep	dithiothreitol,SSC
R9745	T14911	T14912	nummod	10,mM
R9746	T14912	T14910	compound	mM,dithiothreitol
R9747	T14913	T14907	prep	at,SSC
R9748	T14914	T14915	nummod	65,°C
R9749	T14915	T14913	pobj	°C,at
R975	T1902	T1900	punct	", ",produce
R9750	T14916	T14907	prep	for,SSC
R9751	T14917	T14918	nummod	30,min
R9752	T14918	T14916	pobj	min,for
R9753	T14919	T14776	punct	.,washed
R9754	T14921	T14922	aux	To,visualize
R9755	T14922	T14923	advcl	visualize,dipped
R9756	T14924	T14925	det	the,signal
R9757	T14925	T14922	dobj	signal,visualize
R9758	T14926	T14923	punct	", ",dipped
R9759	T14927	T14923	nsubjpass	sections,dipped
R976	T1903	T1904	npadvmod	BMP7,deficient
R9760	T14928	T14923	auxpass	were,dipped
R9761	T14929	T14923	prep	into,dipped
R9762	T14930	T14931	nmod	NTB,emulsion
R9763	T14931	T14929	pobj	emulsion,into
R9764	T14932	T14930	punct	-,NTB
R9765	T14933	T14930	nummod	2,NTB
R9766	T14934	T14935	punct	(,Kodak
R9767	T14935	T14931	parataxis	Kodak,emulsion
R9768	T14936	T14935	punct	", ",Kodak
R9769	T14937	T14935	npadvmod	http://www.Kodak.com,Kodak
R977	T1904	T1906	amod	deficient,embryos
R9770	T14938	T14935	punct	),Kodak
R9771	T14939	T14923	cc	and,dipped
R9772	T14940	T14923	conj	placed,dipped
R9773	T14941	T14940	prep	at,placed
R9774	T14942	T14943	nummod	4,°C
R9775	T14943	T14941	pobj	°C,at
R9776	T14944	T14923	punct	.,dipped
R9777	T14946	T14947	prep	After,counterstained
R9778	T14948	T14946	pcomp	developing,After
R9779	T14949	T14947	punct	", ",counterstained
R978	T1905	T1904	punct	-,deficient
R9780	T14950	T14947	nsubjpass	sections,counterstained
R9781	T14951	T14947	auxpass	were,counterstained
R9782	T14952	T14947	prep	with,counterstained
R9783	T14953	T14952	pobj	H,with
R9784	T14954	T14953	cc	&,H
R9785	T14955	T14953	conj	E,H
R9786	T14956	T14947	punct	.,counterstained
R9787	T14958	T14959	det	The,probes
R9788	T14959	T14964	nsubjpass	probes,transcribed
R9789	T14960	T14961	npadvmod	35S,labeled
R979	T1906	T1900	nsubj	embryos,produce
R9790	T14961	T14959	amod	labeled,probes
R9791	T14962	T14961	punct	-,labeled
R9792	T14963	T14959	compound	cRNA,probes
R9793	T14965	T14964	auxpass	were,transcribed
R9794	T14966	T14964	prep	from,transcribed
R9795	T14967	T14966	pobj	plasmids,from
R9796	T14968	T14967	acl	encoding,plasmids
R9797	T14969	T14970	nmod	type,collagen
R9798	T14970	T14968	dobj	collagen,encoding
R9799	T14971	T14969	nummod	I,type
R98	T260	T261	aux	To,circumvent
R980	T1975	T1966	acomp	responsible,are
R9800	T14972	T14973	punct	[,59
R9801	T14973	T14970	parataxis	59,collagen
R9802	T14974	T14973	punct	],59
R9803	T14975	T14970	punct	", ",collagen
R9804	T14976	T14970	conj	runx2,collagen
R9805	T14977	T14978	punct	[,60
R9806	T14978	T14976	parataxis	60,runx2
R9807	T14979	T14978	punct	],60
R9808	T14980	T14976	punct	", ",runx2
R9809	T14981	T14976	conj	osteopontin,runx2
R981	T1976	T1966	prep	for,are
R9810	T14982	T14983	punct	[,61
R9811	T14983	T14981	parataxis	61,osteopontin
R9812	T14984	T14983	punct	],61
R9813	T14985	T14981	punct	", ",osteopontin
R9814	T14986	T14981	cc	and,osteopontin
R9815	T14987	T14981	conj	osterix,osteopontin
R9816	T14988	T14989	punct	[,46
R9817	T14989	T14987	parataxis	46,osterix
R9818	T14990	T14989	punct	],46
R9819	T14991	T14964	punct	.,transcribed
R982	T1907	T1908	advmod	mostly,normal
R9820	T14993	T14994	nmod	Sense,probes
R9821	T14994	T14997	nsubjpass	probes,synthesized
R9822	T14995	T14993	cc	and,Sense
R9823	T14996	T14993	conj	antisense,Sense
R9824	T14998	T14997	auxpass	were,synthesized
R9825	T14999	T14997	prep	from,synthesized
R9826	T15000	T15001	amod	linearized,plasmids
R9827	T15001	T14999	pobj	plasmids,from
R9828	T15002	T14997	advcl	using,synthesized
R9829	T15003	T15004	det	a,System
R983	T1977	T1976	pcomp	establishing,for
R9830	T15004	T15002	dobj	System,using
R9831	T15005	T15004	compound	Riboprobe,System
R9832	T15006	T15004	compound	Combination,System
R9833	T15007	T15008	punct	(,Promega
R9834	T15008	T15004	parataxis	Promega,System
R9835	T15009	T15008	punct	", ",Promega
R9836	T15010	T15008	npadvmod	http://www.promega.com,Promega
R9837	T15011	T15008	punct	),Promega
R9838	T15012	T14997	punct	.,synthesized
R984	T1978	T1979	compound	digit,identity
R985	T1979	T1977	dobj	identity,establishing
R986	T1980	T1955	punct	", ",seem
R987	T1981	T1982	mark	although,remains
R988	T1908	T1909	amod	normal,limbs
R989	T1982	T1955	advcl	remains,seem
R99	T261	T262	advcl	circumvent,constructed
R990	T1983	T1982	nsubj	redundancy,remains
R991	T1909	T1900	dobj	limbs,produce
R992	T1984	T1983	prep	between,redundancy
R993	T1985	T1984	pobj	BMP2,between
R994	T1986	T1985	punct	", ",BMP2
R995	T1910	T1900	punct	", ",produce
R996	T1987	T1985	conj	BMP4,BMP2
R997	T1988	T1987	punct	", ",BMP4
R998	T1989	T1987	cc	and,BMP4
R999	T1911	T1900	cc	and,produce

PMC:1713256 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

PMC:1713256 JSON TXT 8 Projects