PMC:1635039 / 31414-32327 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T16575 0-12 JJ denotes Histological
T16576 13-21 NNS denotes analyses
T16577 21-126 sentence denotes Embryos (E17) were fixed with 4% paraformaldehyhe for 14 hours, dehydrated and embedded in paraffin wax.
T16578 22-29 NNS denotes Embryos
T16580 30-31 -LRB- denotes (
T16581 31-34 NN denotes E17
T16582 34-35 -RRB- denotes )
T16583 36-40 VBD denotes were
T16579 41-46 VBN denotes fixed
T16584 47-51 IN denotes with
T16585 52-53 CD denotes 4
T16586 53-54 NN denotes %
T16587 55-71 NN denotes paraformaldehyhe
T16588 72-75 IN denotes for
T16589 76-78 CD denotes 14
T16590 79-84 NNS denotes hours
T16591 84-86 , denotes ,
T16592 86-96 VBN denotes dehydrated
T16593 97-100 CC denotes and
T16594 101-109 VBN denotes embedded
T16595 110-112 IN denotes in
T16596 113-121 NN denotes paraffin
T16597 122-125 NN denotes wax
T16598 125-126 . denotes .
T16599 126-209 sentence denotes Sections (7–8 um) were stained with Hematoxylin and Eosin (n≥3 for each genotype).
T16600 127-135 NNS denotes Sections
T16602 136-137 -LRB- denotes (
T16604 137-138 CD denotes 7
T16606 138-139 SYM denotes
T16605 139-140 CD denotes 8
T16603 141-143 NN denotes um
T16607 143-144 -RRB- denotes )
T16608 145-149 VBD denotes were
T16601 150-157 VBN denotes stained
T16609 158-162 IN denotes with
T16610 163-174 NN denotes Hematoxylin
T16611 175-178 CC denotes and
T16612 179-184 NN denotes Eosin
T16613 185-186 -LRB- denotes (
T16615 186-187 NN denotes n
T16616 187-188 SYM denotes
T16614 188-189 CD denotes 3
T16617 190-193 IN denotes for
T16618 194-198 DT denotes each
T16619 199-207 NN denotes genotype
T16620 207-208 -RRB- denotes )
T16621 208-209 . denotes .
T16622 209-308 sentence denotes For lineage tracing analyses, embryos were stained for β-galactosidase activity as described [43].
T16623 210-213 IN denotes For
T16625 214-221 NN denotes lineage
T16626 222-229 NN denotes tracing
T16627 230-238 NNS denotes analyses
T16628 238-240 , denotes ,
T16629 240-247 NNS denotes embryos
T16630 248-252 VBD denotes were
T16624 253-260 VBN denotes stained
T16631 261-264 IN denotes for
T16632 265-266 NN denotes β
T16634 266-267 HYPH denotes -
T16633 267-280 NN denotes galactosidase
T16635 281-289 NN denotes activity
T16636 290-292 IN denotes as
T16637 293-302 VBN denotes described
T16638 303-304 -LRB- denotes [
T16639 304-306 CD denotes 43
T16640 306-307 -RRB- denotes ]
T16641 307-308 . denotes .
T16642 308-541 sentence denotes Briefly, the specimens (E11.0 – E11.5) were fixed in 4% paraformaldehyde for 30 minutes at room temperature, washed 3 times for 10 minutes in the detergent wash and developed for 2–12 hours in X-gal solution (n≥3 for each genotype).
T16643 309-316 RB denotes Briefly
T16645 316-318 , denotes ,
T16646 318-321 DT denotes the
T16647 322-331 NNS denotes specimens
T16648 332-333 -LRB- denotes (
T16649 333-338 NN denotes E11.0
T16650 339-340 SYM denotes
T16651 341-346 NN denotes E11.5
T16652 346-347 -RRB- denotes )
T16653 348-352 VBD denotes were
T16644 353-358 VBN denotes fixed
T16654 359-361 IN denotes in
T16655 362-363 CD denotes 4
T16656 363-364 NN denotes %
T16657 365-381 NN denotes paraformaldehyde
T16658 382-385 IN denotes for
T16659 386-388 CD denotes 30
T16660 389-396 NNS denotes minutes
T16661 397-399 IN denotes at
T16662 400-404 NN denotes room
T16663 405-416 NN denotes temperature
T16664 416-418 , denotes ,
T16665 418-424 VBN denotes washed
T16666 425-426 CD denotes 3
T16667 427-432 NNS denotes times
T16668 433-436 IN denotes for
T16669 437-439 CD denotes 10
T16670 440-447 NNS denotes minutes
T16671 448-450 IN denotes in
T16672 451-454 DT denotes the
T16674 455-464 NN denotes detergent
T16673 465-469 NN denotes wash
T16675 470-473 CC denotes and
T16676 474-483 VBN denotes developed
T16677 484-487 IN denotes for
T16678 488-489 CD denotes 2
T16680 489-490 SYM denotes
T16679 490-492 CD denotes 12
T16681 493-498 NNS denotes hours
T16682 499-501 IN denotes in
T16683 502-503 NN denotes X
T16685 503-504 HYPH denotes -
T16684 504-507 NN denotes gal
T16686 508-516 NN denotes solution
T16687 517-518 -LRB- denotes (
T16689 518-519 NN denotes n
T16690 519-520 SYM denotes
T16688 520-521 CD denotes 3
T16691 522-525 IN denotes for
T16692 526-530 DT denotes each
T16693 531-539 NN denotes genotype
T16694 539-540 -RRB- denotes )
T16695 540-541 . denotes .
T16696 541-765 sentence denotes For immunohistochemistry, fixed sections from tissues harvested at E10.5 – E11.5 were stained with monoclonal α-smooth muscle actin (DAKO), cleaved caspase-3 (Cell Signaling) or phophohistone-H3 (Cell Signaling) antibodies.
T16697 542-545 IN denotes For
T16699 546-566 NN denotes immunohistochemistry
T16700 566-568 , denotes ,
T16701 568-573 VBN denotes fixed
T16702 574-582 NNS denotes sections
T16703 583-587 IN denotes from
T16704 588-595 NNS denotes tissues
T16705 596-605 VBN denotes harvested
T16706 606-608 IN denotes at
T16707 609-614 NN denotes E10.5
T16708 615-616 SYM denotes
T16709 617-622 NN denotes E11.5
T16710 623-627 VBD denotes were
T16698 628-635 VBN denotes stained
T16711 636-640 IN denotes with
T16712 641-651 JJ denotes monoclonal
T16714 652-653 NN denotes α
T16716 653-654 HYPH denotes -
T16717 654-660 JJ denotes smooth
T16715 661-667 NN denotes muscle
T16713 668-673 NN denotes actin
T16719 674-675 -LRB- denotes (
T16720 675-679 NN denotes DAKO
T16721 679-680 -RRB- denotes )
T16722 680-682 , denotes ,
T16723 682-689 VBN denotes cleaved
T16724 690-697 NN denotes caspase
T16725 697-698 HYPH denotes -
T16726 698-699 CD denotes 3
T16727 700-701 -LRB- denotes (
T16729 701-705 NNP denotes Cell
T16728 706-715 NNP denotes Signaling
T16730 715-716 -RRB- denotes )
T16731 717-719 CC denotes or
T16732 720-733 NN denotes phophohistone
T16734 733-734 HYPH denotes -
T16733 734-736 NN denotes H3
T16735 737-738 -LRB- denotes (
T16737 738-742 NNP denotes Cell
T16736 743-752 NNP denotes Signaling
T16738 752-753 -RRB- denotes )
T16718 754-764 NNS denotes antibodies
T16739 764-765 . denotes .
T16740 765-848 sentence denotes TUNEL assays were performed using the DeadEnd fluorometric TUNEL system (Promega).
T16741 766-771 NN denotes TUNEL
T16742 772-778 NNS denotes assays
T16744 779-783 VBD denotes were
T16743 784-793 VBN denotes performed
T16745 794-799 VBG denotes using
T16746 800-803 DT denotes the
T16748 804-811 NNP denotes DeadEnd
T16750 812-824 JJ denotes fluorometric
T16749 825-830 NN denotes TUNEL
T16747 831-837 NN denotes system
T16751 838-839 -LRB- denotes (
T16752 839-846 NNP denotes Promega
T16753 846-847 -RRB- denotes )
T16754 847-848 . denotes .
T16755 848-913 sentence denotes In each assay 3 or more embryos were analyzed for each genotype.
T16756 849-851 IN denotes In
T16758 852-856 DT denotes each
T16759 857-862 NN denotes assay
T16760 863-864 CD denotes 3
T16762 865-867 CC denotes or
T16763 868-872 JJR denotes more
T16761 873-880 NNS denotes embryos
T16764 881-885 VBD denotes were
T16757 886-894 VBN denotes analyzed
T16765 895-898 IN denotes for
T16766 899-903 DT denotes each
T16767 904-912 NN denotes genotype
T16768 912-913 . denotes .
R4984 T16575 T16576 amod Histological,analyses
R4985 T16578 T16579 nsubjpass Embryos,fixed
R4986 T16580 T16581 punct (,E17
R4987 T16581 T16578 parataxis E17,Embryos
R4988 T16582 T16581 punct ),E17
R4989 T16583 T16579 auxpass were,fixed
R4990 T16584 T16579 prep with,fixed
R4991 T16585 T16586 nummod 4,%
R4992 T16586 T16587 compound %,paraformaldehyhe
R4993 T16587 T16584 pobj paraformaldehyhe,with
R4994 T16588 T16579 prep for,fixed
R4995 T16589 T16590 nummod 14,hours
R4996 T16590 T16588 pobj hours,for
R4997 T16591 T16579 punct ", ",fixed
R4998 T16592 T16579 conj dehydrated,fixed
R4999 T16593 T16592 cc and,dehydrated
R5000 T16594 T16592 conj embedded,dehydrated
R5001 T16595 T16594 prep in,embedded
R5002 T16596 T16597 compound paraffin,wax
R5003 T16597 T16595 pobj wax,in
R5004 T16598 T16579 punct .,fixed
R5005 T16600 T16601 nsubjpass Sections,stained
R5006 T16602 T16603 punct (,um
R5007 T16603 T16600 parataxis um,Sections
R5008 T16604 T16605 quantmod 7,8
R5009 T16605 T16603 nummod 8,um
R5010 T16606 T16605 punct –,8
R5011 T16607 T16603 punct ),um
R5012 T16608 T16601 auxpass were,stained
R5013 T16609 T16601 prep with,stained
R5014 T16610 T16609 pobj Hematoxylin,with
R5015 T16611 T16610 cc and,Hematoxylin
R5016 T16612 T16610 conj Eosin,Hematoxylin
R5017 T16613 T16614 punct (,3
R5018 T16614 T16601 parataxis 3,stained
R5019 T16615 T16614 nsubj n,3
R5020 T16616 T16614 punct ≥,3
R5021 T16617 T16614 prep for,3
R5022 T16618 T16619 det each,genotype
R5023 T16619 T16617 pobj genotype,for
R5024 T16620 T16614 punct ),3
R5025 T16621 T16601 punct .,stained
R5026 T16623 T16624 prep For,stained
R5027 T16625 T16626 compound lineage,tracing
R5028 T16626 T16627 compound tracing,analyses
R5029 T16627 T16623 pobj analyses,For
R5030 T16628 T16624 punct ", ",stained
R5031 T16629 T16624 nsubjpass embryos,stained
R5032 T16630 T16624 auxpass were,stained
R5033 T16631 T16624 prep for,stained
R5034 T16632 T16633 compound β,galactosidase
R5035 T16633 T16635 compound galactosidase,activity
R5036 T16634 T16633 punct -,galactosidase
R5037 T16635 T16631 pobj activity,for
R5038 T16636 T16637 mark as,described
R5039 T16637 T16624 advcl described,stained
R5040 T16638 T16639 punct [,43
R5041 T16639 T16624 parataxis 43,stained
R5042 T16640 T16639 punct ],43
R5043 T16641 T16624 punct .,stained
R5044 T16643 T16644 advmod Briefly,fixed
R5045 T16645 T16644 punct ", ",fixed
R5046 T16646 T16647 det the,specimens
R5047 T16647 T16644 nsubjpass specimens,fixed
R5048 T16648 T16649 punct (,E11.0
R5049 T16649 T16647 parataxis E11.0,specimens
R5050 T16650 T16651 punct –,E11.5
R5051 T16651 T16649 prep E11.5,E11.0
R5052 T16652 T16649 punct ),E11.0
R5053 T16653 T16644 auxpass were,fixed
R5054 T16654 T16644 prep in,fixed
R5055 T16655 T16656 nummod 4,%
R5056 T16656 T16657 compound %,paraformaldehyde
R5057 T16657 T16654 pobj paraformaldehyde,in
R5058 T16658 T16644 prep for,fixed
R5059 T16659 T16660 nummod 30,minutes
R5060 T16660 T16658 pobj minutes,for
R5061 T16661 T16644 prep at,fixed
R5062 T16662 T16663 compound room,temperature
R5063 T16663 T16661 pobj temperature,at
R5064 T16664 T16644 punct ", ",fixed
R5065 T16665 T16644 conj washed,fixed
R5066 T16666 T16667 nummod 3,times
R5067 T16667 T16665 npadvmod times,washed
R5068 T16668 T16665 prep for,washed
R5069 T16669 T16670 nummod 10,minutes
R5070 T16670 T16668 pobj minutes,for
R5071 T16671 T16665 prep in,washed
R5072 T16672 T16673 det the,wash
R5073 T16673 T16671 pobj wash,in
R5074 T16674 T16673 compound detergent,wash
R5075 T16675 T16665 cc and,washed
R5076 T16676 T16665 conj developed,washed
R5077 T16677 T16676 prep for,developed
R5078 T16678 T16679 quantmod 2,12
R5079 T16679 T16681 nummod 12,hours
R5080 T16680 T16679 punct –,12
R5081 T16681 T16677 pobj hours,for
R5082 T16682 T16676 prep in,developed
R5083 T16683 T16684 compound X,gal
R5084 T16684 T16686 compound gal,solution
R5085 T16685 T16684 punct -,gal
R5086 T16686 T16682 pobj solution,in
R5087 T16687 T16688 punct (,3
R5088 T16688 T16676 parataxis 3,developed
R5089 T16689 T16688 nsubj n,3
R5090 T16690 T16688 punct ≥,3
R5091 T16691 T16688 prep for,3
R5092 T16692 T16693 det each,genotype
R5093 T16693 T16691 pobj genotype,for
R5094 T16694 T16688 punct ),3
R5095 T16695 T16644 punct .,fixed
R5096 T16697 T16698 prep For,stained
R5097 T16699 T16697 pobj immunohistochemistry,For
R5098 T16700 T16698 punct ", ",stained
R5099 T16701 T16702 amod fixed,sections
R5100 T16702 T16698 nsubjpass sections,stained
R5101 T16703 T16702 prep from,sections
R5102 T16704 T16703 pobj tissues,from
R5103 T16705 T16704 acl harvested,tissues
R5104 T16706 T16705 prep at,harvested
R5105 T16707 T16706 pobj E10.5,at
R5106 T16708 T16709 punct –,E11.5
R5107 T16709 T16707 prep E11.5,E10.5
R5108 T16710 T16698 auxpass were,stained
R5109 T16711 T16698 prep with,stained
R5110 T16712 T16713 amod monoclonal,actin
R5111 T16713 T16718 nmod actin,antibodies
R5112 T16714 T16715 nmod α,muscle
R5113 T16715 T16713 nmod muscle,actin
R5114 T16716 T16715 punct -,muscle
R5115 T16717 T16715 amod smooth,muscle
R5116 T16718 T16711 pobj antibodies,with
R5117 T16719 T16720 punct (,DAKO
R5118 T16720 T16713 parataxis DAKO,actin
R5119 T16721 T16720 punct ),DAKO
R5120 T16722 T16713 punct ", ",actin
R5121 T16723 T16724 amod cleaved,caspase
R5122 T16724 T16713 conj caspase,actin
R5123 T16725 T16724 punct -,caspase
R5124 T16726 T16724 nummod 3,caspase
R5125 T16727 T16728 punct (,Signaling
R5126 T16728 T16724 parataxis Signaling,caspase
R5127 T16729 T16728 compound Cell,Signaling
R5128 T16730 T16728 punct ),Signaling
R5129 T16731 T16724 cc or,caspase
R5130 T16732 T16733 compound phophohistone,H3
R5131 T16733 T16724 conj H3,caspase
R5132 T16734 T16733 punct -,H3
R5133 T16735 T16736 punct (,Signaling
R5134 T16736 T16733 parataxis Signaling,H3
R5135 T16737 T16736 compound Cell,Signaling
R5136 T16738 T16736 punct ),Signaling
R5137 T16739 T16698 punct .,stained
R5138 T16741 T16742 compound TUNEL,assays
R5139 T16742 T16743 nsubjpass assays,performed
R5140 T16744 T16743 auxpass were,performed
R5141 T16745 T16743 advcl using,performed
R5142 T16746 T16747 det the,system
R5143 T16747 T16745 dobj system,using
R5144 T16748 T16749 nmod DeadEnd,TUNEL
R5145 T16749 T16747 compound TUNEL,system
R5146 T16750 T16749 amod fluorometric,TUNEL
R5147 T16751 T16752 punct (,Promega
R5148 T16752 T16745 parataxis Promega,using
R5149 T16753 T16752 punct ),Promega
R5150 T16754 T16743 punct .,performed
R5151 T16756 T16757 prep In,analyzed
R5152 T16758 T16759 det each,assay
R5153 T16759 T16756 pobj assay,In
R5154 T16760 T16761 nummod 3,embryos
R5155 T16761 T16757 nsubjpass embryos,analyzed
R5156 T16762 T16760 cc or,3
R5157 T16763 T16760 conj more,3
R5158 T16764 T16757 auxpass were,analyzed
R5159 T16765 T16757 prep for,analyzed
R5160 T16766 T16767 det each,genotype
R5161 T16767 T16765 pobj genotype,for
R5162 T16768 T16757 punct .,analyzed