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PMC:1635039 / 30579-32974 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T15914 9-13 NN denotes Alk5
T15916 13-14 HYPH denotes /
T15915 14-21 NN denotes Wnt1Cre
T15917 22-26 NNS denotes mice
T15918 26-221 sentence denotes Alk5 (and Alk2) mutant and control embryos were generated by mating Alk5ko/+(Alk2ko/+)/Wnt1-Cre male mice with females homozygous for the Alk5flox (Alk2flox) allele and the R26R reporter[12,19].
T15919 27-31 NN denotes Alk5
T15921 32-33 -LRB- denotes (
T15922 33-36 CC denotes and
T15923 37-41 NN denotes Alk2
T15924 41-42 -RRB- denotes )
T15920 43-49 NN denotes mutant
T15926 50-53 CC denotes and
T15927 54-61 NN denotes control
T15925 62-69 NNS denotes embryos
T15929 70-74 VBD denotes were
T15928 75-84 VBN denotes generated
T15930 85-87 IN denotes by
T15931 88-94 VBG denotes mating
T15932 95-101 NN denotes Alk5ko
T15934 101-102 HYPH denotes /
T15935 102-103 SYM denotes +
T15936 103-104 -LRB- denotes (
T15937 104-110 NN denotes Alk2ko
T15938 110-111 HYPH denotes /
T15939 111-112 SYM denotes +
T15940 112-113 -RRB- denotes )
T15941 113-114 HYPH denotes /
T15942 114-118 NN denotes Wnt1
T15944 118-119 HYPH denotes -
T15943 119-122 NN denotes Cre
T15945 123-127 JJ denotes male
T15933 128-132 NNS denotes mice
T15946 133-137 IN denotes with
T15947 138-145 NNS denotes females
T15948 146-156 JJ denotes homozygous
T15949 157-160 IN denotes for
T15950 161-164 DT denotes the
T15952 165-173 NN denotes Alk5flox
T15953 174-175 -LRB- denotes (
T15954 175-183 NN denotes Alk2flox
T15955 183-184 -RRB- denotes )
T15951 185-191 NN denotes allele
T15956 192-195 CC denotes and
T15957 196-199 DT denotes the
T15959 200-204 NN denotes R26R
T15958 205-213 NN denotes reporter
T15960 213-214 -LRB- denotes [
T15962 214-216 CD denotes 12
T15963 216-217 , denotes ,
T15961 217-219 CD denotes 19
T15964 219-220 -RRB- denotes ]
T15965 220-221 . denotes .
T15966 221-283 sentence denotes Genotyping was performed by PCR as described earlier [21,42].
T15967 222-232 NN denotes Genotyping
T15969 233-236 VBD denotes was
T15968 237-246 VBN denotes performed
T15970 247-249 IN denotes by
T15971 250-253 NN denotes PCR
T15972 254-256 IN denotes as
T15973 257-266 VBN denotes described
T15974 267-274 RBR denotes earlier
T15975 275-276 -LRB- denotes [
T15977 276-278 CD denotes 21
T15978 278-279 , denotes ,
T15976 279-281 CD denotes 42
T15979 281-282 -RRB- denotes ]
T15980 282-283 . denotes .
T15981 283-421 sentence denotes Wnt1-Cre mice were kindly provided by A. McMahon (Harvard University) and R26R reporter mice were obtained from the Jackson laboratories.
T15982 284-288 NN denotes Wnt1
T15984 288-289 HYPH denotes -
T15983 289-292 NN denotes Cre
T15985 293-297 NNS denotes mice
T15987 298-302 VBD denotes were
T15988 303-309 RB denotes kindly
T15986 310-318 VBN denotes provided
T15989 319-321 IN denotes by
T15990 322-324 NNP denotes A.
T15991 325-332 NNP denotes McMahon
T15992 333-334 -LRB- denotes (
T15994 334-341 NNP denotes Harvard
T15993 342-352 NNP denotes University
T15995 352-353 -RRB- denotes )
T15996 354-357 CC denotes and
T15997 358-362 NN denotes R26R
T15998 363-371 NN denotes reporter
T15999 372-376 NNS denotes mice
T16001 377-381 VBD denotes were
T16000 382-390 VBN denotes obtained
T16002 391-395 IN denotes from
T16003 396-399 DT denotes the
T16005 400-407 NNP denotes Jackson
T16004 408-420 NNS denotes laboratories
T16006 420-421 . denotes .
T16007 421-589 sentence denotes All studies were carried out at the Animal Care Facility of the Saban Research Institute of Childrens Hospital Los Angeles in accordance with institutional guidelines.
T16008 422-425 DT denotes All
T16009 426-433 NNS denotes studies
T16011 434-438 VBD denotes were
T16010 439-446 VBN denotes carried
T16012 447-450 RP denotes out
T16013 451-453 IN denotes at
T16014 454-457 DT denotes the
T16016 458-464 NN denotes Animal
T16017 465-469 NN denotes Care
T16015 470-478 NN denotes Facility
T16018 479-481 IN denotes of
T16019 482-485 DT denotes the
T16021 486-491 NNP denotes Saban
T16022 492-500 NNP denotes Research
T16020 501-510 NNP denotes Institute
T16023 511-513 IN denotes of
T16024 514-523 NNP denotes Childrens
T16025 524-532 NNP denotes Hospital
T16026 533-536 NNP denotes Los
T16027 537-544 NNP denotes Angeles
T16028 545-547 IN denotes in
T16029 548-558 NN denotes accordance
T16030 559-563 IN denotes with
T16031 564-577 JJ denotes institutional
T16032 578-588 NNS denotes guidelines
T16033 588-589 . denotes .
T16142 591-596 VBN denotes Timed
T16143 597-605 NNS denotes mataings
T16144 605-733 sentence denotes Mice were mated during the dark period of the controlled light cycle; presence of vaginal plugs was designated as day 0 hour 0.
T16145 606-610 NNS denotes Mice
T16147 611-615 VBD denotes were
T16146 616-621 VBN denotes mated
T16149 622-628 IN denotes during
T16150 629-632 DT denotes the
T16152 633-637 JJ denotes dark
T16151 638-644 NN denotes period
T16153 645-647 IN denotes of
T16154 648-651 DT denotes the
T16156 652-662 VBN denotes controlled
T16157 663-668 NN denotes light
T16155 669-674 NN denotes cycle
T16158 674-675 : denotes ;
T16159 676-684 NN denotes presence
T16160 685-687 IN denotes of
T16161 688-695 JJ denotes vaginal
T16162 696-701 NNS denotes plugs
T16163 702-705 VBD denotes was
T16148 706-716 VBN denotes designated
T16164 717-719 IN denotes as
T16165 720-723 NN denotes day
T16166 724-725 CD denotes 0
T16167 726-730 NN denotes hour
T16168 731-732 CD denotes 0
T16169 732-733 . denotes .
T16170 733-833 sentence denotes Females were euthanized by CO2, and embryos were collected in Hanks' balanced salt solution on ice.
T16171 734-741 NNS denotes Females
T16173 742-746 VBD denotes were
T16172 747-757 VBN denotes euthanized
T16174 758-760 IN denotes by
T16175 761-764 NN denotes CO2
T16176 764-766 , denotes ,
T16177 766-769 CC denotes and
T16178 770-777 NNS denotes embryos
T16180 778-782 VBD denotes were
T16179 783-792 VBN denotes collected
T16181 793-795 IN denotes in
T16182 796-801 NNP denotes Hanks
T16184 801-802 POS denotes '
T16185 803-811 VBN denotes balanced
T16186 812-816 NN denotes salt
T16183 817-825 NN denotes solution
T16187 826-828 IN denotes on
T16188 829-832 NN denotes ice
T16189 832-833 . denotes .
T16575 835-847 JJ denotes Histological
T16576 848-856 NNS denotes analyses
T16577 856-961 sentence denotes Embryos (E17) were fixed with 4% paraformaldehyhe for 14 hours, dehydrated and embedded in paraffin wax.
T16578 857-864 NNS denotes Embryos
T16580 865-866 -LRB- denotes (
T16581 866-869 NN denotes E17
T16582 869-870 -RRB- denotes )
T16583 871-875 VBD denotes were
T16579 876-881 VBN denotes fixed
T16584 882-886 IN denotes with
T16585 887-888 CD denotes 4
T16586 888-889 NN denotes %
T16587 890-906 NN denotes paraformaldehyhe
T16588 907-910 IN denotes for
T16589 911-913 CD denotes 14
T16590 914-919 NNS denotes hours
T16591 919-921 , denotes ,
T16592 921-931 VBN denotes dehydrated
T16593 932-935 CC denotes and
T16594 936-944 VBN denotes embedded
T16595 945-947 IN denotes in
T16596 948-956 NN denotes paraffin
T16597 957-960 NN denotes wax
T16598 960-961 . denotes .
T16599 961-1044 sentence denotes Sections (7–8 um) were stained with Hematoxylin and Eosin (n≥3 for each genotype).
T16600 962-970 NNS denotes Sections
T16602 971-972 -LRB- denotes (
T16604 972-973 CD denotes 7
T16606 973-974 SYM denotes –
T16605 974-975 CD denotes 8
T16603 976-978 NN denotes um
T16607 978-979 -RRB- denotes )
T16608 980-984 VBD denotes were
T16601 985-992 VBN denotes stained
T16609 993-997 IN denotes with
T16610 998-1009 NN denotes Hematoxylin
T16611 1010-1013 CC denotes and
T16612 1014-1019 NN denotes Eosin
T16613 1020-1021 -LRB- denotes (
T16615 1021-1022 NN denotes n
T16616 1022-1023 SYM denotes ≥
T16614 1023-1024 CD denotes 3
T16617 1025-1028 IN denotes for
T16618 1029-1033 DT denotes each
T16619 1034-1042 NN denotes genotype
T16620 1042-1043 -RRB- denotes )
T16621 1043-1044 . denotes .
T16622 1044-1143 sentence denotes For lineage tracing analyses, embryos were stained for β-galactosidase activity as described [43].
T16623 1045-1048 IN denotes For
T16625 1049-1056 NN denotes lineage
T16626 1057-1064 NN denotes tracing
T16627 1065-1073 NNS denotes analyses
T16628 1073-1075 , denotes ,
T16629 1075-1082 NNS denotes embryos
T16630 1083-1087 VBD denotes were
T16624 1088-1095 VBN denotes stained
T16631 1096-1099 IN denotes for
T16632 1100-1101 NN denotes β
T16634 1101-1102 HYPH denotes -
T16633 1102-1115 NN denotes galactosidase
T16635 1116-1124 NN denotes activity
T16636 1125-1127 IN denotes as
T16637 1128-1137 VBN denotes described
T16638 1138-1139 -LRB- denotes [
T16639 1139-1141 CD denotes 43
T16640 1141-1142 -RRB- denotes ]
T16641 1142-1143 . denotes .
T16642 1143-1376 sentence denotes Briefly, the specimens (E11.0 – E11.5) were fixed in 4% paraformaldehyde for 30 minutes at room temperature, washed 3 times for 10 minutes in the detergent wash and developed for 2–12 hours in X-gal solution (n≥3 for each genotype).
T16643 1144-1151 RB denotes Briefly
T16645 1151-1153 , denotes ,
T16646 1153-1156 DT denotes the
T16647 1157-1166 NNS denotes specimens
T16648 1167-1168 -LRB- denotes (
T16649 1168-1173 NN denotes E11.0
T16650 1174-1175 SYM denotes –
T16651 1176-1181 NN denotes E11.5
T16652 1181-1182 -RRB- denotes )
T16653 1183-1187 VBD denotes were
T16644 1188-1193 VBN denotes fixed
T16654 1194-1196 IN denotes in
T16655 1197-1198 CD denotes 4
T16656 1198-1199 NN denotes %
T16657 1200-1216 NN denotes paraformaldehyde
T16658 1217-1220 IN denotes for
T16659 1221-1223 CD denotes 30
T16660 1224-1231 NNS denotes minutes
T16661 1232-1234 IN denotes at
T16662 1235-1239 NN denotes room
T16663 1240-1251 NN denotes temperature
T16664 1251-1253 , denotes ,
T16665 1253-1259 VBN denotes washed
T16666 1260-1261 CD denotes 3
T16667 1262-1267 NNS denotes times
T16668 1268-1271 IN denotes for
T16669 1272-1274 CD denotes 10
T16670 1275-1282 NNS denotes minutes
T16671 1283-1285 IN denotes in
T16672 1286-1289 DT denotes the
T16674 1290-1299 NN denotes detergent
T16673 1300-1304 NN denotes wash
T16675 1305-1308 CC denotes and
T16676 1309-1318 VBN denotes developed
T16677 1319-1322 IN denotes for
T16678 1323-1324 CD denotes 2
T16680 1324-1325 SYM denotes –
T16679 1325-1327 CD denotes 12
T16681 1328-1333 NNS denotes hours
T16682 1334-1336 IN denotes in
T16683 1337-1338 NN denotes X
T16685 1338-1339 HYPH denotes -
T16684 1339-1342 NN denotes gal
T16686 1343-1351 NN denotes solution
T16687 1352-1353 -LRB- denotes (
T16689 1353-1354 NN denotes n
T16690 1354-1355 SYM denotes ≥
T16688 1355-1356 CD denotes 3
T16691 1357-1360 IN denotes for
T16692 1361-1365 DT denotes each
T16693 1366-1374 NN denotes genotype
T16694 1374-1375 -RRB- denotes )
T16695 1375-1376 . denotes .
T16696 1376-1600 sentence denotes For immunohistochemistry, fixed sections from tissues harvested at E10.5 – E11.5 were stained with monoclonal α-smooth muscle actin (DAKO), cleaved caspase-3 (Cell Signaling) or phophohistone-H3 (Cell Signaling) antibodies.
T16697 1377-1380 IN denotes For
T16699 1381-1401 NN denotes immunohistochemistry
T16700 1401-1403 , denotes ,
T16701 1403-1408 VBN denotes fixed
T16702 1409-1417 NNS denotes sections
T16703 1418-1422 IN denotes from
T16704 1423-1430 NNS denotes tissues
T16705 1431-1440 VBN denotes harvested
T16706 1441-1443 IN denotes at
T16707 1444-1449 NN denotes E10.5
T16708 1450-1451 SYM denotes –
T16709 1452-1457 NN denotes E11.5
T16710 1458-1462 VBD denotes were
T16698 1463-1470 VBN denotes stained
T16711 1471-1475 IN denotes with
T16712 1476-1486 JJ denotes monoclonal
T16714 1487-1488 NN denotes α
T16716 1488-1489 HYPH denotes -
T16717 1489-1495 JJ denotes smooth
T16715 1496-1502 NN denotes muscle
T16713 1503-1508 NN denotes actin
T16719 1509-1510 -LRB- denotes (
T16720 1510-1514 NN denotes DAKO
T16721 1514-1515 -RRB- denotes )
T16722 1515-1517 , denotes ,
T16723 1517-1524 VBN denotes cleaved
T16724 1525-1532 NN denotes caspase
T16725 1532-1533 HYPH denotes -
T16726 1533-1534 CD denotes 3
T16727 1535-1536 -LRB- denotes (
T16729 1536-1540 NNP denotes Cell
T16728 1541-1550 NNP denotes Signaling
T16730 1550-1551 -RRB- denotes )
T16731 1552-1554 CC denotes or
T16732 1555-1568 NN denotes phophohistone
T16734 1568-1569 HYPH denotes -
T16733 1569-1571 NN denotes H3
T16735 1572-1573 -LRB- denotes (
T16737 1573-1577 NNP denotes Cell
T16736 1578-1587 NNP denotes Signaling
T16738 1587-1588 -RRB- denotes )
T16718 1589-1599 NNS denotes antibodies
T16739 1599-1600 . denotes .
T16740 1600-1683 sentence denotes TUNEL assays were performed using the DeadEnd fluorometric TUNEL system (Promega).
T16741 1601-1606 NN denotes TUNEL
T16742 1607-1613 NNS denotes assays
T16744 1614-1618 VBD denotes were
T16743 1619-1628 VBN denotes performed
T16745 1629-1634 VBG denotes using
T16746 1635-1638 DT denotes the
T16748 1639-1646 NNP denotes DeadEnd
T16750 1647-1659 JJ denotes fluorometric
T16749 1660-1665 NN denotes TUNEL
T16747 1666-1672 NN denotes system
T16751 1673-1674 -LRB- denotes (
T16752 1674-1681 NNP denotes Promega
T16753 1681-1682 -RRB- denotes )
T16754 1682-1683 . denotes .
T16755 1683-1748 sentence denotes In each assay 3 or more embryos were analyzed for each genotype.
T16756 1684-1686 IN denotes In
T16758 1687-1691 DT denotes each
T16759 1692-1697 NN denotes assay
T16760 1698-1699 CD denotes 3
T16762 1700-1702 CC denotes or
T16763 1703-1707 JJR denotes more
T16761 1708-1715 NNS denotes embryos
T16764 1716-1720 VBD denotes were
T16757 1721-1729 VBN denotes analyzed
T16765 1730-1733 IN denotes for
T16766 1734-1738 DT denotes each
T16767 1739-1747 NN denotes genotype
T16768 1747-1748 . denotes .
T16958 1750-1753 NN denotes Ink
T16960 1754-1757 CC denotes and
T16961 1758-1765 NN denotes casting
T16962 1766-1769 NN denotes dye
T16959 1770-1780 NNS denotes injections
T16963 1780-1885 sentence denotes Embryos (E10.0 – E13.0) were dissected and placed in ice cold PBS (n≥3 per genotype in each time point).
T16964 1781-1788 NNS denotes Embryos
T16966 1789-1790 -LRB- denotes (
T16967 1790-1795 NN denotes E10.0
T16968 1796-1797 SYM denotes –
T16969 1798-1803 NN denotes E13.0
T16970 1803-1804 -RRB- denotes )
T16971 1805-1809 VBD denotes were
T16965 1810-1819 VBN denotes dissected
T16972 1820-1823 CC denotes and
T16973 1824-1830 VBN denotes placed
T16974 1831-1833 IN denotes in
T16975 1834-1837 NN denotes ice
T16977 1838-1842 JJ denotes cold
T16976 1843-1846 NN denotes PBS
T16978 1847-1848 -LRB- denotes (
T16980 1848-1849 NN denotes n
T16981 1849-1850 SYM denotes ≥
T16979 1850-1851 CD denotes 3
T16982 1852-1855 IN denotes per
T16983 1856-1864 NN denotes genotype
T16984 1865-1867 IN denotes in
T16985 1868-1872 DT denotes each
T16987 1873-1877 NN denotes time
T16986 1878-1883 NN denotes point
T16988 1883-1884 -RRB- denotes )
T16989 1884-1885 . denotes .
T16990 1885-2059 sentence denotes Using a pulled glass pipette, India ink or Yellow casting dye (Connecticut Valley Biological Supply) was injected into the ventricles until ink/dye penetrated small vessels.
T16991 1886-1891 VBG denotes Using
T16993 1892-1893 DT denotes a
T16995 1894-1900 VBN denotes pulled
T16996 1901-1906 NN denotes glass
T16994 1907-1914 NN denotes pipette
T16997 1914-1916 , denotes ,
T16998 1916-1921 NNP denotes India
T16999 1922-1925 NN denotes ink
T17000 1926-1928 CC denotes or
T17001 1929-1935 JJ denotes Yellow
T17003 1936-1943 NN denotes casting
T17002 1944-1947 NN denotes dye
T17004 1948-1949 -LRB- denotes (
T17006 1949-1960 NNP denotes Connecticut
T17007 1961-1967 NNP denotes Valley
T17008 1968-1978 NNP denotes Biological
T17005 1979-1985 NNP denotes Supply
T17009 1985-1986 -RRB- denotes )
T17010 1987-1990 VBD denotes was
T16992 1991-1999 VBN denotes injected
T17011 2000-2004 IN denotes into
T17012 2005-2008 DT denotes the
T17013 2009-2019 NNS denotes ventricles
T17014 2020-2025 IN denotes until
T17016 2026-2029 NN denotes ink
T17018 2029-2030 HYPH denotes /
T17017 2030-2033 NN denotes dye
T17015 2034-2044 VBD denotes penetrated
T17019 2045-2050 JJ denotes small
T17020 2051-2058 NNS denotes vessels
T17021 2058-2059 . denotes .
T17022 2059-2188 sentence denotes Embryos were postfixed in 10% buffered formalin for 12 hours, dehydrated and cleared in benzylbenzoate: benzyl alcohol (2:1v/v).
T17023 2060-2067 NNS denotes Embryos
T17025 2068-2072 VBD denotes were
T17024 2073-2082 VBN denotes postfixed
T17026 2083-2085 IN denotes in
T17027 2086-2088 CD denotes 10
T17028 2088-2089 NN denotes %
T17030 2090-2098 VBN denotes buffered
T17029 2099-2107 NN denotes formalin
T17031 2108-2111 IN denotes for
T17032 2112-2114 CD denotes 12
T17033 2115-2120 NNS denotes hours
T17034 2120-2122 , denotes ,
T17035 2122-2132 VBN denotes dehydrated
T17036 2133-2136 CC denotes and
T17037 2137-2144 VBN denotes cleared
T17038 2145-2147 IN denotes in
T17039 2148-2162 NN denotes benzylbenzoate
T17040 2162-2164 : denotes :
T17041 2164-2170 NN denotes benzyl
T17042 2171-2178 NN denotes alcohol
T17043 2179-2180 -LRB- denotes (
T17044 2180-2181 CD denotes 2
T17045 2181-2182 SYM denotes :
T17047 2182-2184 NN denotes 1v
T17048 2184-2185 HYPH denotes /
T17046 2185-2186 NN denotes v
T17049 2186-2187 -RRB- denotes )
T17050 2187-2188 . denotes .
T17140 2190-2200 NN denotes Expression
T17141 2201-2209 NNS denotes analyses
T17142 2209-2395 sentence denotes To visualize parathyroid hormone expression we used in situ hybridization and an antisense probe corresponding to nucleotides 97–534 (kindly provided by Nancy Manley) as described [44].
T17143 2210-2212 TO denotes To
T17144 2213-2222 VB denotes visualize
T17146 2223-2234 NN denotes parathyroid
T17147 2235-2242 NN denotes hormone
T17148 2243-2253 NN denotes expression
T17149 2254-2256 PRP denotes we
T17145 2257-2261 VBD denotes used
T17150 2262-2264 FW denotes in
T17151 2265-2269 FW denotes situ
T17152 2270-2283 NN denotes hybridization
T17153 2284-2287 CC denotes and
T17154 2288-2290 DT denotes an
T17156 2291-2300 NN denotes antisense
T17155 2301-2306 NN denotes probe
T17157 2307-2320 VBG denotes corresponding
T17158 2321-2323 IN denotes to
T17159 2324-2335 NNS denotes nucleotides
T17160 2336-2338 CD denotes 97
T17162 2338-2339 SYM denotes –
T17161 2339-2342 CD denotes 534
T17163 2343-2344 -LRB- denotes (
T17164 2344-2350 RB denotes kindly
T17165 2351-2359 VBN denotes provided
T17166 2360-2362 IN denotes by
T17167 2363-2368 NNP denotes Nancy
T17168 2369-2375 NNP denotes Manley
T17169 2375-2376 -RRB- denotes )
T17170 2377-2379 IN denotes as
T17171 2380-2389 VBN denotes described
T17172 2390-2391 -LRB- denotes [
T17173 2391-2393 CD denotes 44
T17174 2393-2394 -RRB- denotes ]
T17175 2394-2395 . denotes .
R4830 T15914 T15915 compound Alk5,Wnt1Cre
R4831 T15915 T15917 compound Wnt1Cre,mice
R4832 T15916 T15915 punct /,Wnt1Cre
R4833 T15919 T15920 nmod Alk5,mutant
R4834 T15920 T15925 nmod mutant,embryos
R4835 T15921 T15919 punct (,Alk5
R4836 T15922 T15919 cc and,Alk5
R4837 T15923 T15919 conj Alk2,Alk5
R4838 T15924 T15920 punct ),mutant
R4839 T15925 T15928 nsubjpass embryos,generated
R4840 T15926 T15920 cc and,mutant
R4841 T15927 T15920 conj control,mutant
R4842 T15929 T15928 auxpass were,generated
R4843 T15930 T15928 agent by,generated
R4844 T15931 T15930 pcomp mating,by
R4845 T15932 T15933 nmod Alk5ko,mice
R4846 T15933 T15931 dobj mice,mating
R4847 T15934 T15932 punct /,Alk5ko
R4848 T15935 T15932 punct +,Alk5ko
R4849 T15936 T15937 punct (,Alk2ko
R4850 T15937 T15932 parataxis Alk2ko,Alk5ko
R4851 T15938 T15937 punct /,Alk2ko
R4852 T15939 T15937 punct +,Alk2ko
R4853 T15940 T15937 punct ),Alk2ko
R4854 T15941 T15932 punct /,Alk5ko
R4855 T15942 T15943 compound Wnt1,Cre
R4856 T15943 T15932 appos Cre,Alk5ko
R4857 T15944 T15943 punct -,Cre
R4858 T15945 T15933 amod male,mice
R4859 T15946 T15931 prep with,mating
R4860 T15947 T15946 pobj females,with
R4861 T15948 T15947 amod homozygous,females
R4862 T15949 T15948 prep for,homozygous
R4863 T15950 T15951 det the,allele
R4864 T15951 T15949 pobj allele,for
R4865 T15952 T15951 nmod Alk5flox,allele
R4866 T15953 T15954 punct (,Alk2flox
R4867 T15954 T15952 parataxis Alk2flox,Alk5flox
R4868 T15955 T15954 punct ),Alk2flox
R4869 T15956 T15951 cc and,allele
R4870 T15957 T15958 det the,reporter
R4871 T15958 T15951 conj reporter,allele
R4872 T15959 T15958 compound R26R,reporter
R4873 T15960 T15961 punct [,19
R4874 T15961 T15931 parataxis 19,mating
R4875 T15962 T15961 nummod 12,19
R4876 T15963 T15961 punct ",",19
R4877 T15964 T15961 punct ],19
R4878 T15965 T15928 punct .,generated
R4879 T15967 T15968 nsubjpass Genotyping,performed
R4880 T15969 T15968 auxpass was,performed
R4881 T15970 T15968 prep by,performed
R4882 T15971 T15970 pobj PCR,by
R4883 T15972 T15973 mark as,described
R4884 T15973 T15968 advcl described,performed
R4885 T15974 T15973 advmod earlier,described
R4886 T15975 T15976 punct [,42
R4887 T15976 T15973 parataxis 42,described
R4888 T15977 T15976 nummod 21,42
R4889 T15978 T15976 punct ",",42
R4890 T15979 T15976 punct ],42
R4891 T15980 T15968 punct .,performed
R4892 T15982 T15983 compound Wnt1,Cre
R4893 T15983 T15985 compound Cre,mice
R4894 T15984 T15983 punct -,Cre
R4895 T15985 T15986 nsubjpass mice,provided
R4896 T15987 T15986 auxpass were,provided
R4897 T15988 T15986 advmod kindly,provided
R4898 T15989 T15986 agent by,provided
R4899 T15990 T15991 compound A.,McMahon
R4900 T15991 T15989 pobj McMahon,by
R4901 T15992 T15993 punct (,University
R4902 T15993 T15986 parataxis University,provided
R4903 T15994 T15993 compound Harvard,University
R4904 T15995 T15993 punct ),University
R4905 T15996 T15986 cc and,provided
R4906 T15997 T15998 compound R26R,reporter
R4907 T15998 T15999 compound reporter,mice
R4908 T15999 T16000 nsubjpass mice,obtained
R4909 T16000 T15986 conj obtained,provided
R4910 T16001 T16000 auxpass were,obtained
R4911 T16002 T16000 prep from,obtained
R4912 T16003 T16004 det the,laboratories
R4913 T16004 T16002 pobj laboratories,from
R4914 T16005 T16004 compound Jackson,laboratories
R4915 T16006 T16000 punct .,obtained
R4916 T16008 T16009 det All,studies
R4917 T16009 T16010 nsubjpass studies,carried
R4918 T16011 T16010 auxpass were,carried
R4919 T16012 T16010 prt out,carried
R4920 T16013 T16010 prep at,carried
R4921 T16014 T16015 det the,Facility
R4922 T16015 T16013 pobj Facility,at
R4923 T16016 T16017 compound Animal,Care
R4924 T16017 T16015 compound Care,Facility
R4925 T16018 T16015 prep of,Facility
R4926 T16019 T16020 det the,Institute
R4927 T16020 T16018 pobj Institute,of
R4928 T16021 T16020 compound Saban,Institute
R4929 T16022 T16020 compound Research,Institute
R4930 T16023 T16020 prep of,Institute
R4931 T16024 T16025 compound Childrens,Hospital
R4932 T16025 T16023 pobj Hospital,of
R4933 T16026 T16027 compound Los,Angeles
R4934 T16027 T16025 npadvmod Angeles,Hospital
R4935 T16028 T16010 prep in,carried
R4936 T16029 T16028 pobj accordance,in
R4937 T16030 T16029 prep with,accordance
R4938 T16031 T16032 amod institutional,guidelines
R4939 T16032 T16030 pobj guidelines,with
R4940 T16033 T16010 punct .,carried
R4941 T16142 T16143 amod Timed,mataings
R4942 T16145 T16146 nsubjpass Mice,mated
R4943 T16146 T16148 ccomp mated,designated
R4944 T16147 T16146 auxpass were,mated
R4945 T16149 T16146 prep during,mated
R4946 T16150 T16151 det the,period
R4947 T16151 T16149 pobj period,during
R4948 T16152 T16151 amod dark,period
R4949 T16153 T16151 prep of,period
R4950 T16154 T16155 det the,cycle
R4951 T16155 T16153 pobj cycle,of
R4952 T16156 T16155 amod controlled,cycle
R4953 T16157 T16155 compound light,cycle
R4954 T16158 T16148 punct ;,designated
R4955 T16159 T16148 nsubjpass presence,designated
R4956 T16160 T16159 prep of,presence
R4957 T16161 T16162 amod vaginal,plugs
R4958 T16162 T16160 pobj plugs,of
R4959 T16163 T16148 auxpass was,designated
R4960 T16164 T16148 prep as,designated
R4961 T16165 T16164 pobj day,as
R4962 T16166 T16165 nummod 0,day
R4963 T16167 T16165 npadvmod hour,day
R4964 T16168 T16167 nummod 0,hour
R4965 T16169 T16148 punct .,designated
R4966 T16171 T16172 nsubjpass Females,euthanized
R4967 T16173 T16172 auxpass were,euthanized
R4968 T16174 T16172 prep by,euthanized
R4969 T16175 T16174 pobj CO2,by
R4970 T16176 T16172 punct ", ",euthanized
R4971 T16177 T16172 cc and,euthanized
R4972 T16178 T16179 nsubjpass embryos,collected
R4973 T16179 T16172 conj collected,euthanized
R4974 T16180 T16179 auxpass were,collected
R4975 T16181 T16179 prep in,collected
R4976 T16182 T16183 poss Hanks,solution
R4977 T16183 T16181 pobj solution,in
R4978 T16184 T16182 case ',Hanks
R4979 T16185 T16183 amod balanced,solution
R4980 T16186 T16183 compound salt,solution
R4981 T16187 T16183 prep on,solution
R4982 T16188 T16187 pobj ice,on
R4983 T16189 T16179 punct .,collected
R4984 T16575 T16576 amod Histological,analyses
R4985 T16578 T16579 nsubjpass Embryos,fixed
R4986 T16580 T16581 punct (,E17
R4987 T16581 T16578 parataxis E17,Embryos
R4988 T16582 T16581 punct ),E17
R4989 T16583 T16579 auxpass were,fixed
R4990 T16584 T16579 prep with,fixed
R4991 T16585 T16586 nummod 4,%
R4992 T16586 T16587 compound %,paraformaldehyhe
R4993 T16587 T16584 pobj paraformaldehyhe,with
R4994 T16588 T16579 prep for,fixed
R4995 T16589 T16590 nummod 14,hours
R4996 T16590 T16588 pobj hours,for
R4997 T16591 T16579 punct ", ",fixed
R4998 T16592 T16579 conj dehydrated,fixed
R4999 T16593 T16592 cc and,dehydrated
R5000 T16594 T16592 conj embedded,dehydrated
R5001 T16595 T16594 prep in,embedded
R5002 T16596 T16597 compound paraffin,wax
R5003 T16597 T16595 pobj wax,in
R5004 T16598 T16579 punct .,fixed
R5005 T16600 T16601 nsubjpass Sections,stained
R5006 T16602 T16603 punct (,um
R5007 T16603 T16600 parataxis um,Sections
R5008 T16604 T16605 quantmod 7,8
R5009 T16605 T16603 nummod 8,um
R5010 T16606 T16605 punct –,8
R5011 T16607 T16603 punct ),um
R5012 T16608 T16601 auxpass were,stained
R5013 T16609 T16601 prep with,stained
R5014 T16610 T16609 pobj Hematoxylin,with
R5015 T16611 T16610 cc and,Hematoxylin
R5016 T16612 T16610 conj Eosin,Hematoxylin
R5017 T16613 T16614 punct (,3
R5018 T16614 T16601 parataxis 3,stained
R5019 T16615 T16614 nsubj n,3
R5020 T16616 T16614 punct ≥,3
R5021 T16617 T16614 prep for,3
R5022 T16618 T16619 det each,genotype
R5023 T16619 T16617 pobj genotype,for
R5024 T16620 T16614 punct ),3
R5025 T16621 T16601 punct .,stained
R5026 T16623 T16624 prep For,stained
R5027 T16625 T16626 compound lineage,tracing
R5028 T16626 T16627 compound tracing,analyses
R5029 T16627 T16623 pobj analyses,For
R5030 T16628 T16624 punct ", ",stained
R5031 T16629 T16624 nsubjpass embryos,stained
R5032 T16630 T16624 auxpass were,stained
R5033 T16631 T16624 prep for,stained
R5034 T16632 T16633 compound β,galactosidase
R5035 T16633 T16635 compound galactosidase,activity
R5036 T16634 T16633 punct -,galactosidase
R5037 T16635 T16631 pobj activity,for
R5038 T16636 T16637 mark as,described
R5039 T16637 T16624 advcl described,stained
R5040 T16638 T16639 punct [,43
R5041 T16639 T16624 parataxis 43,stained
R5042 T16640 T16639 punct ],43
R5043 T16641 T16624 punct .,stained
R5044 T16643 T16644 advmod Briefly,fixed
R5045 T16645 T16644 punct ", ",fixed
R5046 T16646 T16647 det the,specimens
R5047 T16647 T16644 nsubjpass specimens,fixed
R5048 T16648 T16649 punct (,E11.0
R5049 T16649 T16647 parataxis E11.0,specimens
R5050 T16650 T16651 punct –,E11.5
R5051 T16651 T16649 prep E11.5,E11.0
R5052 T16652 T16649 punct ),E11.0
R5053 T16653 T16644 auxpass were,fixed
R5054 T16654 T16644 prep in,fixed
R5055 T16655 T16656 nummod 4,%
R5056 T16656 T16657 compound %,paraformaldehyde
R5057 T16657 T16654 pobj paraformaldehyde,in
R5058 T16658 T16644 prep for,fixed
R5059 T16659 T16660 nummod 30,minutes
R5060 T16660 T16658 pobj minutes,for
R5061 T16661 T16644 prep at,fixed
R5062 T16662 T16663 compound room,temperature
R5063 T16663 T16661 pobj temperature,at
R5064 T16664 T16644 punct ", ",fixed
R5065 T16665 T16644 conj washed,fixed
R5066 T16666 T16667 nummod 3,times
R5067 T16667 T16665 npadvmod times,washed
R5068 T16668 T16665 prep for,washed
R5069 T16669 T16670 nummod 10,minutes
R5070 T16670 T16668 pobj minutes,for
R5071 T16671 T16665 prep in,washed
R5072 T16672 T16673 det the,wash
R5073 T16673 T16671 pobj wash,in
R5074 T16674 T16673 compound detergent,wash
R5075 T16675 T16665 cc and,washed
R5076 T16676 T16665 conj developed,washed
R5077 T16677 T16676 prep for,developed
R5078 T16678 T16679 quantmod 2,12
R5079 T16679 T16681 nummod 12,hours
R5080 T16680 T16679 punct –,12
R5081 T16681 T16677 pobj hours,for
R5082 T16682 T16676 prep in,developed
R5083 T16683 T16684 compound X,gal
R5084 T16684 T16686 compound gal,solution
R5085 T16685 T16684 punct -,gal
R5086 T16686 T16682 pobj solution,in
R5087 T16687 T16688 punct (,3
R5088 T16688 T16676 parataxis 3,developed
R5089 T16689 T16688 nsubj n,3
R5090 T16690 T16688 punct ≥,3
R5091 T16691 T16688 prep for,3
R5092 T16692 T16693 det each,genotype
R5093 T16693 T16691 pobj genotype,for
R5094 T16694 T16688 punct ),3
R5095 T16695 T16644 punct .,fixed
R5096 T16697 T16698 prep For,stained
R5097 T16699 T16697 pobj immunohistochemistry,For
R5098 T16700 T16698 punct ", ",stained
R5099 T16701 T16702 amod fixed,sections
R5100 T16702 T16698 nsubjpass sections,stained
R5101 T16703 T16702 prep from,sections
R5102 T16704 T16703 pobj tissues,from
R5103 T16705 T16704 acl harvested,tissues
R5104 T16706 T16705 prep at,harvested
R5105 T16707 T16706 pobj E10.5,at
R5106 T16708 T16709 punct –,E11.5
R5107 T16709 T16707 prep E11.5,E10.5
R5108 T16710 T16698 auxpass were,stained
R5109 T16711 T16698 prep with,stained
R5110 T16712 T16713 amod monoclonal,actin
R5111 T16713 T16718 nmod actin,antibodies
R5112 T16714 T16715 nmod α,muscle
R5113 T16715 T16713 nmod muscle,actin
R5114 T16716 T16715 punct -,muscle
R5115 T16717 T16715 amod smooth,muscle
R5116 T16718 T16711 pobj antibodies,with
R5117 T16719 T16720 punct (,DAKO
R5118 T16720 T16713 parataxis DAKO,actin
R5119 T16721 T16720 punct ),DAKO
R5120 T16722 T16713 punct ", ",actin
R5121 T16723 T16724 amod cleaved,caspase
R5122 T16724 T16713 conj caspase,actin
R5123 T16725 T16724 punct -,caspase
R5124 T16726 T16724 nummod 3,caspase
R5125 T16727 T16728 punct (,Signaling
R5126 T16728 T16724 parataxis Signaling,caspase
R5127 T16729 T16728 compound Cell,Signaling
R5128 T16730 T16728 punct ),Signaling
R5129 T16731 T16724 cc or,caspase
R5130 T16732 T16733 compound phophohistone,H3
R5131 T16733 T16724 conj H3,caspase
R5132 T16734 T16733 punct -,H3
R5133 T16735 T16736 punct (,Signaling
R5134 T16736 T16733 parataxis Signaling,H3
R5135 T16737 T16736 compound Cell,Signaling
R5136 T16738 T16736 punct ),Signaling
R5137 T16739 T16698 punct .,stained
R5138 T16741 T16742 compound TUNEL,assays
R5139 T16742 T16743 nsubjpass assays,performed
R5140 T16744 T16743 auxpass were,performed
R5141 T16745 T16743 advcl using,performed
R5142 T16746 T16747 det the,system
R5143 T16747 T16745 dobj system,using
R5144 T16748 T16749 nmod DeadEnd,TUNEL
R5145 T16749 T16747 compound TUNEL,system
R5146 T16750 T16749 amod fluorometric,TUNEL
R5147 T16751 T16752 punct (,Promega
R5148 T16752 T16745 parataxis Promega,using
R5149 T16753 T16752 punct ),Promega
R5150 T16754 T16743 punct .,performed
R5151 T16756 T16757 prep In,analyzed
R5152 T16758 T16759 det each,assay
R5153 T16759 T16756 pobj assay,In
R5154 T16760 T16761 nummod 3,embryos
R5155 T16761 T16757 nsubjpass embryos,analyzed
R5156 T16762 T16760 cc or,3
R5157 T16763 T16760 conj more,3
R5158 T16764 T16757 auxpass were,analyzed
R5159 T16765 T16757 prep for,analyzed
R5160 T16766 T16767 det each,genotype
R5161 T16767 T16765 pobj genotype,for
R5162 T16768 T16757 punct .,analyzed
R5163 T16958 T16959 nmod Ink,injections
R5164 T16960 T16958 cc and,Ink
R5165 T16961 T16962 compound casting,dye
R5166 T16962 T16958 conj dye,Ink
R5167 T16964 T16965 nsubjpass Embryos,dissected
R5168 T16966 T16967 punct (,E10.0
R5169 T16967 T16964 parataxis E10.0,Embryos
R5170 T16968 T16969 punct –,E13.0
R5171 T16969 T16967 prep E13.0,E10.0
R5172 T16970 T16967 punct ),E10.0
R5173 T16971 T16965 auxpass were,dissected
R5174 T16972 T16965 cc and,dissected
R5175 T16973 T16965 conj placed,dissected
R5176 T16974 T16973 prep in,placed
R5177 T16975 T16976 nmod ice,PBS
R5178 T16976 T16974 pobj PBS,in
R5179 T16977 T16976 amod cold,PBS
R5180 T16978 T16979 punct (,3
R5181 T16979 T16973 parataxis 3,placed
R5182 T16980 T16979 nsubj n,3
R5183 T16981 T16979 punct ≥,3
R5184 T16982 T16979 prep per,3
R5185 T16983 T16982 pobj genotype,per
R5186 T16984 T16979 prep in,3
R5187 T16985 T16986 det each,point
R5188 T16986 T16984 pobj point,in
R5189 T16987 T16986 compound time,point
R5190 T16988 T16979 punct ),3
R5191 T16989 T16965 punct .,dissected
R5192 T16991 T16992 advcl Using,injected
R5193 T16993 T16994 det a,pipette
R5194 T16994 T16991 dobj pipette,Using
R5195 T16995 T16994 amod pulled,pipette
R5196 T16996 T16994 compound glass,pipette
R5197 T16997 T16992 punct ", ",injected
R5198 T16998 T16999 compound India,ink
R5199 T16999 T16992 nsubjpass ink,injected
R5200 T17000 T16999 cc or,ink
R5201 T17001 T17002 amod Yellow,dye
R5202 T17002 T16999 conj dye,ink
R5203 T17003 T17002 compound casting,dye
R5204 T17004 T17005 punct (,Supply
R5205 T17005 T17002 parataxis Supply,dye
R5206 T17006 T17007 compound Connecticut,Valley
R5207 T17007 T17005 compound Valley,Supply
R5208 T17008 T17005 compound Biological,Supply
R5209 T17009 T17005 punct ),Supply
R5210 T17010 T16992 auxpass was,injected
R5211 T17011 T16992 prep into,injected
R5212 T17012 T17013 det the,ventricles
R5213 T17013 T17011 pobj ventricles,into
R5214 T17014 T17015 mark until,penetrated
R5215 T17015 T16992 advcl penetrated,injected
R5216 T17016 T17017 compound ink,dye
R5217 T17017 T17015 nsubj dye,penetrated
R5218 T17018 T17017 punct /,dye
R5219 T17019 T17020 amod small,vessels
R5220 T17020 T17015 dobj vessels,penetrated
R5221 T17021 T16992 punct .,injected
R5222 T17023 T17024 nsubjpass Embryos,postfixed
R5223 T17025 T17024 auxpass were,postfixed
R5224 T17026 T17024 prep in,postfixed
R5225 T17027 T17028 nummod 10,%
R5226 T17028 T17029 nmod %,formalin
R5227 T17029 T17026 pobj formalin,in
R5228 T17030 T17029 amod buffered,formalin
R5229 T17031 T17024 prep for,postfixed
R5230 T17032 T17033 nummod 12,hours
R5231 T17033 T17031 pobj hours,for
R5232 T17034 T17024 punct ", ",postfixed
R5233 T17035 T17024 conj dehydrated,postfixed
R5234 T17036 T17035 cc and,dehydrated
R5235 T17037 T17035 conj cleared,dehydrated
R5236 T17038 T17037 prep in,cleared
R5237 T17039 T17038 pobj benzylbenzoate,in
R5238 T17040 T17039 punct : ,benzylbenzoate
R5239 T17041 T17042 compound benzyl,alcohol
R5240 T17042 T17039 appos alcohol,benzylbenzoate
R5241 T17043 T17044 punct (,2
R5242 T17044 T17039 parataxis 2,benzylbenzoate
R5243 T17045 T17046 punct :,v
R5244 T17046 T17044 prep v,2
R5245 T17047 T17046 compound 1v,v
R5246 T17048 T17046 punct /,v
R5247 T17049 T17044 punct ),2
R5248 T17050 T17024 punct .,postfixed
R5249 T17140 T17141 compound Expression,analyses
R5250 T17143 T17144 aux To,visualize
R5251 T17144 T17145 advcl visualize,used
R5252 T17146 T17147 compound parathyroid,hormone
R5253 T17147 T17148 compound hormone,expression
R5254 T17148 T17144 dobj expression,visualize
R5255 T17149 T17145 nsubj we,used
R5256 T17150 T17151 nmod in,situ
R5257 T17151 T17152 nmod situ,hybridization
R5258 T17152 T17145 dobj hybridization,used
R5259 T17153 T17152 cc and,hybridization
R5260 T17154 T17155 det an,probe
R5261 T17155 T17152 conj probe,hybridization
R5262 T17156 T17155 compound antisense,probe
R5263 T17157 T17155 acl corresponding,probe
R5264 T17158 T17157 prep to,corresponding
R5265 T17159 T17158 pobj nucleotides,to
R5266 T17160 T17161 quantmod 97,534
R5267 T17161 T17159 nummod 534,nucleotides
R5268 T17162 T17161 punct –,534
R5269 T17163 T17159 punct (,nucleotides
R5270 T17164 T17165 advmod kindly,provided
R5271 T17165 T17159 acl provided,nucleotides
R5272 T17166 T17165 agent by,provided
R5273 T17167 T17168 compound Nancy,Manley
R5274 T17168 T17166 pobj Manley,by
R5275 T17169 T17145 punct ),used
R5276 T17170 T17171 mark as,described
R5277 T17171 T17145 advcl described,used
R5278 T17172 T17173 punct [,44
R5279 T17173 T17171 parataxis 44,described
R5280 T17174 T17173 punct ],44
R5281 T17175 T17145 punct .,used