PMC:1635039 JSON TXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T513	0-9	JJ	denotes	Defective
T515	0-138	sentence	denotes	Defective ALK5 signaling in the neural crest leads to increased postmigratory neural crest cell apoptosis and severe outflow tract defects
T516	10-14	NN	denotes	ALK5
T514	15-24	NN	denotes	signaling
T518	25-27	IN	denotes	in
T519	28-31	DT	denotes	the
T521	32-38	JJ	denotes	neural
T520	39-44	NN	denotes	crest
T517	45-50	VBZ	denotes	leads
T522	51-53	IN	denotes	to
T523	54-63	VBN	denotes	increased
T525	64-77	JJ	denotes	postmigratory
T526	78-84	JJ	denotes	neural
T528	85-90	NN	denotes	crest
T527	91-95	NN	denotes	cell
T524	96-105	NN	denotes	apoptosis
T529	106-109	CC	denotes	and
T530	110-116	JJ	denotes	severe
T532	117-124	NN	denotes	outflow
T533	125-130	NN	denotes	tract
T531	131-138	NNS	denotes	defects
T534	138-139	sentence	denotes
T535	149-159	sentence	denotes	Background
T536	149-159	NN	denotes	Background
T537	159-247	sentence	denotes	Congenital cardiovascular diseases are the most common form of birth defects in humans.
T538	160-170	JJ	denotes	Congenital
T540	171-185	JJ	denotes	cardiovascular
T539	186-194	NNS	denotes	diseases
T541	195-198	VBP	denotes	are
T542	199-202	DT	denotes	the
T544	203-207	RBS	denotes	most
T545	208-214	JJ	denotes	common
T543	215-219	NN	denotes	form
T546	220-222	IN	denotes	of
T547	223-228	NN	denotes	birth
T548	229-236	NNS	denotes	defects
T549	237-239	IN	denotes	in
T550	240-246	NNS	denotes	humans
T551	246-247	.	denotes	.
T552	247-426	sentence	denotes	A substantial portion of these defects has been associated with inappropriate induction, migration, differentiation and patterning of pluripotent cardiac neural crest stem cells.
T553	248-249	DT	denotes	A
T555	250-261	JJ	denotes	substantial
T554	262-269	NN	denotes	portion
T557	270-272	IN	denotes	of
T558	273-278	DT	denotes	these
T559	279-286	NNS	denotes	defects
T560	287-290	VBZ	denotes	has
T561	291-295	VBN	denotes	been
T556	296-306	VBN	denotes	associated
T562	307-311	IN	denotes	with
T563	312-325	JJ	denotes	inappropriate
T564	326-335	NN	denotes	induction
T565	335-337	,	denotes	,
T566	337-346	NN	denotes	migration
T567	346-348	,	denotes	,
T568	348-363	NN	denotes	differentiation
T569	364-367	CC	denotes	and
T570	368-378	NN	denotes	patterning
T571	379-381	IN	denotes	of
T572	382-393	JJ	denotes	pluripotent
T574	394-401	JJ	denotes	cardiac
T576	402-408	JJ	denotes	neural
T575	409-414	NN	denotes	crest
T577	415-419	NN	denotes	stem
T573	420-425	NNS	denotes	cells
T578	425-426	.	denotes	.
T579	426-604	sentence	denotes	While TGF-β-superfamily signaling has been strongly implicated in neural crest cell development, the detailed molecular signaling mechanisms in vivo are still poorly understood.
T580	427-432	IN	denotes	While
T582	433-436	NN	denotes	TGF
T584	436-437	HYPH	denotes	-
T583	437-438	NN	denotes	β
T586	438-439	HYPH	denotes	-
T585	439-450	NN	denotes	superfamily
T587	451-460	NN	denotes	signaling
T588	461-464	VBZ	denotes	has
T589	465-469	VBN	denotes	been
T590	470-478	RB	denotes	strongly
T581	479-489	VBN	denotes	implicated
T592	490-492	IN	denotes	in
T593	493-499	JJ	denotes	neural
T595	500-505	NN	denotes	crest
T594	506-510	NN	denotes	cell
T596	511-522	NN	denotes	development
T597	522-524	,	denotes	,
T598	524-527	DT	denotes	the
T600	528-536	JJ	denotes	detailed
T601	537-546	JJ	denotes	molecular
T602	547-556	NN	denotes	signaling
T599	557-567	NNS	denotes	mechanisms
T603	568-570	FW	denotes	in
T604	571-575	FW	denotes	vivo
T605	576-579	VBP	denotes	are
T606	580-585	RB	denotes	still
T607	586-592	RB	denotes	poorly
T591	593-603	VBN	denotes	understood
T608	603-604	.	denotes	.
T609	604-613	sentence	denotes	Results
T610	606-613	NNS	denotes	Results
T611	613-708	sentence	denotes	We deleted the TGF-β type I receptor Alk5 specifically in the mouse neural crest cell lineage.
T612	614-616	PRP	denotes	We
T613	617-624	VBD	denotes	deleted
T614	625-628	DT	denotes	the
T616	629-632	NN	denotes	TGF
T618	632-633	HYPH	denotes	-
T617	633-634	NN	denotes	β
T619	635-639	NN	denotes	type
T620	640-641	CD	denotes	I
T621	642-650	NN	denotes	receptor
T615	651-655	NN	denotes	Alk5
T622	656-668	RB	denotes	specifically
T623	669-671	IN	denotes	in
T624	672-675	DT	denotes	the
T626	676-681	NN	denotes	mouse
T627	682-688	JJ	denotes	neural
T629	689-694	NN	denotes	crest
T628	695-699	NN	denotes	cell
T625	700-707	NN	denotes	lineage
T630	707-708	.	denotes	.
T631	708-963	sentence	denotes	Failure in signaling via ALK5 leads to severe cardiovascular and pharyngeal defects, including inappropriate remodeling of pharyngeal arch arteries, abnormal aortic sac development, failure in pharyngeal organ migration and persistent truncus arteriosus.
T632	709-716	NN	denotes	Failure
T634	717-719	IN	denotes	in
T635	720-729	NN	denotes	signaling
T636	730-733	IN	denotes	via
T637	734-738	NN	denotes	ALK5
T633	739-744	VBZ	denotes	leads
T638	745-747	IN	denotes	to
T639	748-754	JJ	denotes	severe
T641	755-769	JJ	denotes	cardiovascular
T642	770-773	CC	denotes	and
T643	774-784	JJ	denotes	pharyngeal
T640	785-792	NNS	denotes	defects
T644	792-794	,	denotes	,
T645	794-803	VBG	denotes	including
T646	804-817	JJ	denotes	inappropriate
T647	818-828	NN	denotes	remodeling
T648	829-831	IN	denotes	of
T649	832-842	JJ	denotes	pharyngeal
T651	843-847	NN	denotes	arch
T650	848-856	NNS	denotes	arteries
T652	856-858	,	denotes	,
T653	858-866	JJ	denotes	abnormal
T655	867-873	JJ	denotes	aortic
T656	874-877	NN	denotes	sac
T654	878-889	NN	denotes	development
T657	889-891	,	denotes	,
T658	891-898	NN	denotes	failure
T659	899-901	IN	denotes	in
T660	902-912	JJ	denotes	pharyngeal
T662	913-918	NN	denotes	organ
T661	919-928	NN	denotes	migration
T663	929-932	CC	denotes	and
T664	933-943	JJ	denotes	persistent
T666	944-951	NN	denotes	truncus
T665	952-962	NN	denotes	arteriosus
T667	962-963	.	denotes	.
T668	963-1141	sentence	denotes	While ALK5 is not required for neural crest cell migration, our results demonstrate that it plays an important role in the survival of post-migratory cardiac neural crest cells.
T669	964-969	IN	denotes	While
T671	970-974	NN	denotes	ALK5
T672	975-977	VBZ	denotes	is
T673	978-981	RB	denotes	not
T670	982-990	VBN	denotes	required
T675	991-994	IN	denotes	for
T676	995-1001	JJ	denotes	neural
T678	1002-1007	NN	denotes	crest
T677	1008-1012	NN	denotes	cell
T679	1013-1022	NN	denotes	migration
T680	1022-1024	,	denotes	,
T681	1024-1027	PRP$	denotes	our
T682	1028-1035	NNS	denotes	results
T674	1036-1047	VBP	denotes	demonstrate
T683	1048-1052	IN	denotes	that
T685	1053-1055	PRP	denotes	it
T684	1056-1061	VBZ	denotes	plays
T686	1062-1064	DT	denotes	an
T688	1065-1074	JJ	denotes	important
T687	1075-1079	NN	denotes	role
T689	1080-1082	IN	denotes	in
T690	1083-1086	DT	denotes	the
T691	1087-1095	NN	denotes	survival
T692	1096-1098	IN	denotes	of
T693	1099-1113	JJ	denotes	post-migratory
T695	1114-1121	JJ	denotes	cardiac
T697	1122-1128	JJ	denotes	neural
T696	1129-1134	NN	denotes	crest
T694	1135-1140	NNS	denotes	cells
T698	1140-1141	.	denotes	.
T699	1141-1153	sentence	denotes	Conclusion
T700	1143-1153	NN	denotes	Conclusion
T701	1153-1325	sentence	denotes	Our results demonstrate that ALK5-mediated signaling in neural crest cells plays an essential cell-autonomous role in the pharyngeal and cardiac outflow tract development.
T702	1154-1157	PRP$	denotes	Our
T703	1158-1165	NNS	denotes	results
T704	1166-1177	VBP	denotes	demonstrate
T705	1178-1182	IN	denotes	that
T707	1183-1187	NN	denotes	ALK5
T709	1187-1188	HYPH	denotes	-
T708	1188-1196	VBN	denotes	mediated
T710	1197-1206	NN	denotes	signaling
T711	1207-1209	IN	denotes	in
T712	1210-1216	JJ	denotes	neural
T714	1217-1222	NN	denotes	crest
T713	1223-1228	NNS	denotes	cells
T706	1229-1234	VBZ	denotes	plays
T715	1235-1237	DT	denotes	an
T717	1238-1247	JJ	denotes	essential
T718	1248-1252	NN	denotes	cell
T720	1252-1253	HYPH	denotes	-
T719	1253-1263	JJ	denotes	autonomous
T716	1264-1268	NN	denotes	role
T721	1269-1271	IN	denotes	in
T722	1272-1275	DT	denotes	the
T724	1276-1286	JJ	denotes	pharyngeal
T726	1287-1290	CC	denotes	and
T727	1291-1298	JJ	denotes	cardiac
T728	1299-1306	NN	denotes	outflow
T725	1307-1312	NN	denotes	tract
T723	1313-1324	NN	denotes	development
T729	1324-1325	.	denotes	.
T2694	1338-1339	DT	denotes	A
T2696	1340-1352	JJ	denotes	considerable
T2695	1353-1363	NN	denotes	percentage
T2698	1364-1366	IN	denotes	of
T2699	1367-1374	JJ	denotes	cardiac
T2701	1375-1380	NN	denotes	birth
T2700	1381-1388	NNS	denotes	defects
T2702	1389-1391	VBZ	denotes	is
T2697	1392-1398	VBN	denotes	caused
T2703	1399-1401	IN	denotes	by
T2704	1402-1403	DT	denotes	a
T2705	1404-1411	NN	denotes	failure
T2706	1412-1414	IN	denotes	in
T2707	1415-1421	JJ	denotes	normal
T2708	1422-1431	NN	denotes	migration
T2709	1431-1433	,	denotes	,
T2710	1433-1448	NN	denotes	differentiation
T2711	1449-1451	CC	denotes	or
T2712	1452-1462	NN	denotes	patterning
T2713	1463-1465	IN	denotes	of
T2714	1466-1469	DT	denotes	the
T2716	1470-1477	JJ	denotes	cardiac
T2717	1478-1484	JJ	denotes	neural
T2715	1485-1490	NN	denotes	crest
T2718	1491-1492	-LRB-	denotes	(
T2719	1492-1495	NN	denotes	CNC
T2720	1495-1496	-RRB-	denotes	)
T2721	1496-1497	.	denotes	.
T2722	1497-1656	sentence	denotes	This subset of pluripotent neural crest stem cells forms in the dorsal aspect of the neural tube at the level of the mid-otic placode to the third somite [1].
T2723	1498-1502	DT	denotes	This
T2724	1503-1509	NN	denotes	subset
T2726	1510-1512	IN	denotes	of
T2727	1513-1524	JJ	denotes	pluripotent
T2729	1525-1531	JJ	denotes	neural
T2728	1532-1537	NN	denotes	crest
T2731	1538-1542	NN	denotes	stem
T2730	1543-1548	NNS	denotes	cells
T2725	1549-1554	VBZ	denotes	forms
T2732	1555-1557	IN	denotes	in
T2733	1558-1561	DT	denotes	the
T2735	1562-1568	JJ	denotes	dorsal
T2734	1569-1575	NN	denotes	aspect
T2736	1576-1578	IN	denotes	of
T2737	1579-1582	DT	denotes	the
T2739	1583-1589	JJ	denotes	neural
T2738	1590-1594	NN	denotes	tube
T2740	1595-1597	IN	denotes	at
T2741	1598-1601	DT	denotes	the
T2742	1602-1607	NN	denotes	level
T2743	1608-1610	IN	denotes	of
T2744	1611-1614	DT	denotes	the
T2746	1615-1623	JJ	denotes	mid-otic
T2745	1624-1631	NN	denotes	placode
T2747	1632-1634	IN	denotes	to
T2748	1635-1638	DT	denotes	the
T2750	1639-1644	JJ	denotes	third
T2749	1645-1651	NN	denotes	somite
T2751	1652-1653	-LRB-	denotes	[
T2752	1653-1654	CD	denotes	1
T2753	1654-1655	-RRB-	denotes	]
T2754	1655-1656	.	denotes	.
T2755	1656-2027	sentence	denotes	Subsequently cardiac neural crest cells (CNCCs) delaminate, undergo a phenotypic transformation from an epithelial to mesenchymal cell type, and migrate latero-ventrally into the 3rd, 4th and 6th pharyngeal arch arteries (PAAs), where they contribute to the formation of the smooth muscle cell layer of endothelial structures derived from the aortic arch arteries [1-3].
T2756	1657-1669	RB	denotes	Subsequently
T2758	1670-1677	JJ	denotes	cardiac
T2760	1678-1684	JJ	denotes	neural
T2761	1685-1690	NN	denotes	crest
T2759	1691-1696	NNS	denotes	cells
T2762	1697-1698	-LRB-	denotes	(
T2763	1698-1703	NNS	denotes	CNCCs
T2764	1703-1704	-RRB-	denotes	)
T2757	1705-1715	VBP	denotes	delaminate
T2765	1715-1717	,	denotes	,
T2766	1717-1724	VBP	denotes	undergo
T2767	1725-1726	DT	denotes	a
T2769	1727-1737	JJ	denotes	phenotypic
T2768	1738-1752	NN	denotes	transformation
T2770	1753-1757	IN	denotes	from
T2771	1758-1760	DT	denotes	an
T2773	1761-1771	JJ	denotes	epithelial
T2774	1772-1774	IN	denotes	to
T2775	1775-1786	JJ	denotes	mesenchymal
T2776	1787-1791	NN	denotes	cell
T2772	1792-1796	NN	denotes	type
T2777	1796-1798	,	denotes	,
T2778	1798-1801	CC	denotes	and
T2779	1802-1809	VBP	denotes	migrate
T2780	1810-1816	RB	denotes	latero
T2782	1816-1817	HYPH	denotes	-
T2781	1817-1826	RB	denotes	ventrally
T2783	1827-1831	IN	denotes	into
T2784	1832-1835	DT	denotes	the
T2786	1836-1839	JJ	denotes	3rd
T2787	1839-1841	,	denotes	,
T2788	1841-1844	JJ	denotes	4th
T2789	1845-1848	CC	denotes	and
T2790	1849-1852	JJ	denotes	6th
T2791	1853-1863	JJ	denotes	pharyngeal
T2792	1864-1868	NN	denotes	arch
T2785	1869-1877	NNS	denotes	arteries
T2793	1878-1879	-LRB-	denotes	(
T2794	1879-1883	NNS	denotes	PAAs
T2795	1883-1884	-RRB-	denotes	)
T2796	1884-1886	,	denotes	,
T2797	1886-1891	WRB	denotes	where
T2799	1892-1896	PRP	denotes	they
T2798	1897-1907	VBP	denotes	contribute
T2800	1908-1910	IN	denotes	to
T2801	1911-1914	DT	denotes	the
T2802	1915-1924	NN	denotes	formation
T2803	1925-1927	IN	denotes	of
T2804	1928-1931	DT	denotes	the
T2806	1932-1938	JJ	denotes	smooth
T2808	1939-1945	NN	denotes	muscle
T2807	1946-1950	NN	denotes	cell
T2805	1951-1956	NN	denotes	layer
T2809	1957-1959	IN	denotes	of
T2810	1960-1971	JJ	denotes	endothelial
T2811	1972-1982	NNS	denotes	structures
T2812	1983-1990	VBN	denotes	derived
T2813	1991-1995	IN	denotes	from
T2814	1996-1999	DT	denotes	the
T2816	2000-2006	JJ	denotes	aortic
T2817	2007-2011	NN	denotes	arch
T2815	2012-2020	NNS	denotes	arteries
T2818	2021-2022	-LRB-	denotes	[
T2819	2022-2023	CD	denotes	1
T2820	2023-2024	SYM	denotes	-
T2821	2024-2025	CD	denotes	3
T2822	2025-2026	-RRB-	denotes	]
T2823	2026-2027	.	denotes	.
T2824	2027-2206	sentence	denotes	A subset of CNCCs continues to migrate deeper into the aortic sac to form the aortico-pulmonary septum; a vital structure, which separates the pulmonary trunk from the aorta [4].
T2825	2028-2029	DT	denotes	A
T2826	2030-2036	NN	denotes	subset
T2828	2037-2039	IN	denotes	of
T2829	2040-2045	NNS	denotes	CNCCs
T2827	2046-2055	VBZ	denotes	continues
T2830	2056-2058	TO	denotes	to
T2831	2059-2066	VB	denotes	migrate
T2832	2067-2073	RBR	denotes	deeper
T2833	2074-2078	IN	denotes	into
T2834	2079-2082	DT	denotes	the
T2836	2083-2089	JJ	denotes	aortic
T2835	2090-2093	NN	denotes	sac
T2837	2094-2096	TO	denotes	to
T2838	2097-2101	VB	denotes	form
T2839	2102-2105	DT	denotes	the
T2841	2106-2113	JJ	denotes	aortico
T2843	2113-2114	HYPH	denotes	-
T2842	2114-2123	JJ	denotes	pulmonary
T2840	2124-2130	NN	denotes	septum
T2844	2130-2131	:	denotes	;
T2845	2132-2133	DT	denotes	a
T2847	2134-2139	JJ	denotes	vital
T2846	2140-2149	NN	denotes	structure
T2848	2149-2151	,	denotes	,
T2849	2151-2156	WDT	denotes	which
T2850	2157-2166	VBZ	denotes	separates
T2851	2167-2170	DT	denotes	the
T2853	2171-2180	JJ	denotes	pulmonary
T2852	2181-2186	NN	denotes	trunk
T2854	2187-2191	IN	denotes	from
T2855	2192-2195	DT	denotes	the
T2856	2196-2201	NN	denotes	aorta
T2857	2202-2203	-LRB-	denotes	[
T2858	2203-2204	CD	denotes	4
T2859	2204-2205	-RRB-	denotes	]
T2860	2205-2206	.	denotes	.
T2861	2206-2574	sentence	denotes	An indispensable role of CNCCs in the development of the cardiac outflow tract was originally demonstrated by pioneering studies of Kirby and coworkers [1], who showed that ablation of the CNC in the chick led to severe outflow tract (OFT) defects including persistent truncus arteriosus (PTA), mispatterning of the great vessels and outflow tract mal-alignments [5].
T2862	2207-2209	DT	denotes	An
T2864	2210-2223	JJ	denotes	indispensable
T2863	2224-2228	NN	denotes	role
T2866	2229-2231	IN	denotes	of
T2867	2232-2237	NNS	denotes	CNCCs
T2868	2238-2240	IN	denotes	in
T2869	2241-2244	DT	denotes	the
T2870	2245-2256	NN	denotes	development
T2871	2257-2259	IN	denotes	of
T2872	2260-2263	DT	denotes	the
T2874	2264-2271	JJ	denotes	cardiac
T2875	2272-2279	NN	denotes	outflow
T2873	2280-2285	NN	denotes	tract
T2876	2286-2289	VBD	denotes	was
T2877	2290-2300	RB	denotes	originally
T2865	2301-2313	VBN	denotes	demonstrated
T2878	2314-2316	IN	denotes	by
T2879	2317-2327	VBG	denotes	pioneering
T2880	2328-2335	NNS	denotes	studies
T2881	2336-2338	IN	denotes	of
T2882	2339-2344	NNP	denotes	Kirby
T2883	2345-2348	CC	denotes	and
T2884	2349-2358	NNS	denotes	coworkers
T2885	2359-2360	-LRB-	denotes	[
T2886	2360-2361	CD	denotes	1
T2887	2361-2362	-RRB-	denotes	]
T2888	2362-2364	,	denotes	,
T2889	2364-2367	WP	denotes	who
T2890	2368-2374	VBD	denotes	showed
T2891	2375-2379	IN	denotes	that
T2893	2380-2388	NN	denotes	ablation
T2894	2389-2391	IN	denotes	of
T2895	2392-2395	DT	denotes	the
T2896	2396-2399	NN	denotes	CNC
T2897	2400-2402	IN	denotes	in
T2898	2403-2406	DT	denotes	the
T2899	2407-2412	NN	denotes	chick
T2892	2413-2416	VBD	denotes	led
T2900	2417-2419	IN	denotes	to
T2901	2420-2426	JJ	denotes	severe
T2903	2427-2434	NN	denotes	outflow
T2902	2435-2440	NN	denotes	tract
T2904	2441-2442	-LRB-	denotes	(
T2905	2442-2445	NN	denotes	OFT
T2906	2445-2446	-RRB-	denotes	)
T2907	2447-2454	VBZ	denotes	defects
T2908	2455-2464	VBG	denotes	including
T2909	2465-2475	JJ	denotes	persistent
T2911	2476-2483	NN	denotes	truncus
T2910	2484-2494	NN	denotes	arteriosus
T2912	2495-2496	-LRB-	denotes	(
T2913	2496-2499	NN	denotes	PTA
T2914	2499-2500	-RRB-	denotes	)
T2915	2500-2502	,	denotes	,
T2916	2502-2515	VBG	denotes	mispatterning
T2917	2516-2518	IN	denotes	of
T2918	2519-2522	DT	denotes	the
T2920	2523-2528	JJ	denotes	great
T2919	2529-2536	NNS	denotes	vessels
T2921	2537-2540	CC	denotes	and
T2922	2541-2548	NN	denotes	outflow
T2923	2549-2554	NN	denotes	tract
T2924	2555-2569	NNS	denotes	mal-alignments
T2925	2570-2571	-LRB-	denotes	[
T2926	2571-2572	CD	denotes	5
T2927	2572-2573	-RRB-	denotes	]
T2928	2573-2574	.	denotes	.
T2929	2574-2773	sentence	denotes	Early migratory CNCCs have been shown to retain a considerable degree of plasticity and their fate is largely controlled by instructional signals from local environments into which NCCs migrate [6].
T2930	2575-2580	RB	denotes	Early
T2932	2581-2590	JJ	denotes	migratory
T2931	2591-2596	NNS	denotes	CNCCs
T2934	2597-2601	VBP	denotes	have
T2935	2602-2606	VBN	denotes	been
T2933	2607-2612	VBN	denotes	shown
T2936	2613-2615	TO	denotes	to
T2937	2616-2622	VB	denotes	retain
T2938	2623-2624	DT	denotes	a
T2940	2625-2637	JJ	denotes	considerable
T2939	2638-2644	NN	denotes	degree
T2941	2645-2647	IN	denotes	of
T2942	2648-2658	NN	denotes	plasticity
T2943	2659-2662	CC	denotes	and
T2944	2663-2668	PRP$	denotes	their
T2945	2669-2673	NN	denotes	fate
T2947	2674-2676	VBZ	denotes	is
T2948	2677-2684	RB	denotes	largely
T2946	2685-2695	VBN	denotes	controlled
T2949	2696-2698	IN	denotes	by
T2950	2699-2712	JJ	denotes	instructional
T2951	2713-2720	NNS	denotes	signals
T2952	2721-2725	IN	denotes	from
T2953	2726-2731	JJ	denotes	local
T2954	2732-2744	NNS	denotes	environments
T2955	2745-2749	IN	denotes	into
T2957	2750-2755	WDT	denotes	which
T2958	2756-2760	NNS	denotes	NCCs
T2956	2761-2768	VBP	denotes	migrate
T2959	2769-2770	-LRB-	denotes	[
T2960	2770-2771	CD	denotes	6
T2961	2771-2772	-RRB-	denotes	]
T2962	2772-2773	.	denotes	.
T2963	2773-2928	sentence	denotes	Several recent studies have indicated that members of the TGF-β superfamily, i.e., TGF-βs and BMPs are likely candidates to provide some of these signals.
T2964	2774-2781	JJ	denotes	Several
T2966	2782-2788	JJ	denotes	recent
T2965	2789-2796	NNS	denotes	studies
T2968	2797-2801	VBP	denotes	have
T2967	2802-2811	VBN	denotes	indicated
T2969	2812-2816	IN	denotes	that
T2971	2817-2824	NNS	denotes	members
T2972	2825-2827	IN	denotes	of
T2973	2828-2831	DT	denotes	the
T2975	2832-2835	NN	denotes	TGF
T2977	2835-2836	HYPH	denotes	-
T2976	2836-2837	NN	denotes	β
T2974	2838-2849	NN	denotes	superfamily
T2978	2849-2851	,	denotes	,
T2979	2851-2855	FW	denotes	i.e.
T2981	2855-2857	,	denotes	,
T2982	2857-2860	NN	denotes	TGF
T2983	2860-2861	HYPH	denotes	-
T2980	2861-2863	NNS	denotes	βs
T2984	2864-2867	CC	denotes	and
T2985	2868-2872	NNS	denotes	BMPs
T2970	2873-2876	VBP	denotes	are
T2986	2877-2883	JJ	denotes	likely
T2987	2884-2894	NNS	denotes	candidates
T2988	2895-2897	TO	denotes	to
T2989	2898-2905	VB	denotes	provide
T2990	2906-2910	DT	denotes	some
T2991	2911-2913	IN	denotes	of
T2992	2914-2919	DT	denotes	these
T2993	2920-2927	NNS	denotes	signals
T2994	2927-2928	.	denotes	.
T2995	2928-3137	sentence	denotes	Mice deficient in TGF-β2 display fourth aortic arch artery defects [7], while neural crest cell specific abrogation of TGF-β type II receptor (Tgfbr2) results in interruption of the aortic arch and PTA [8,9].
T2996	2929-2933	NNS	denotes	Mice
T2998	2934-2943	JJ	denotes	deficient
T2999	2944-2946	IN	denotes	in
T3000	2947-2950	NN	denotes	TGF
T3002	2950-2951	HYPH	denotes	-
T3001	2951-2953	NN	denotes	β2
T2997	2954-2961	VBP	denotes	display
T3003	2962-2968	JJ	denotes	fourth
T3005	2969-2975	JJ	denotes	aortic
T3007	2976-2980	NN	denotes	arch
T3006	2981-2987	NN	denotes	artery
T3004	2988-2995	NNS	denotes	defects
T3008	2996-2997	-LRB-	denotes	[
T3009	2997-2998	CD	denotes	7
T3010	2998-2999	-RRB-	denotes	]
T3011	2999-3001	,	denotes	,
T3012	3001-3006	IN	denotes	while
T3014	3007-3013	JJ	denotes	neural
T3016	3014-3019	NN	denotes	crest
T3015	3020-3024	NN	denotes	cell
T3017	3025-3033	JJ	denotes	specific
T3018	3034-3044	NN	denotes	abrogation
T3019	3045-3047	IN	denotes	of
T3020	3048-3051	NN	denotes	TGF
T3022	3051-3052	HYPH	denotes	-
T3021	3052-3053	NN	denotes	β
T3024	3054-3058	NN	denotes	type
T3025	3059-3061	CD	denotes	II
T3023	3062-3070	NN	denotes	receptor
T3026	3071-3072	-LRB-	denotes	(
T3027	3072-3078	NN	denotes	Tgfbr2
T3028	3078-3079	-RRB-	denotes	)
T3013	3080-3087	VBZ	denotes	results
T3029	3088-3090	IN	denotes	in
T3030	3091-3103	NN	denotes	interruption
T3031	3104-3106	IN	denotes	of
T3032	3107-3110	DT	denotes	the
T3034	3111-3117	JJ	denotes	aortic
T3033	3118-3122	NN	denotes	arch
T3035	3123-3126	CC	denotes	and
T3036	3127-3130	NN	denotes	PTA
T3037	3131-3132	-LRB-	denotes	[
T3039	3132-3133	CD	denotes	8
T3040	3133-3134	,	denotes	,
T3038	3134-3135	CD	denotes	9
T3041	3135-3136	-RRB-	denotes	]
T3042	3136-3137	.	denotes	.
T3043	3137-3313	sentence	denotes	BMPs 6 and -7 are required for proper formation of the outflow tract cushions [10], while BMP type II receptor is needed for proper development of the conotruncal ridges [11].
T3044	3138-3142	NNS	denotes	BMPs
T3045	3143-3144	CD	denotes	6
T3047	3145-3148	CC	denotes	and
T3048	3149-3150	HYPH	denotes	-
T3049	3150-3151	CD	denotes	7
T3050	3152-3155	VBP	denotes	are
T3046	3156-3164	VBN	denotes	required
T3051	3165-3168	IN	denotes	for
T3052	3169-3175	JJ	denotes	proper
T3053	3176-3185	NN	denotes	formation
T3054	3186-3188	IN	denotes	of
T3055	3189-3192	DT	denotes	the
T3057	3193-3200	NN	denotes	outflow
T3058	3201-3206	NN	denotes	tract
T3056	3207-3215	NNS	denotes	cushions
T3059	3216-3217	-LRB-	denotes	[
T3060	3217-3219	CD	denotes	10
T3061	3219-3220	-RRB-	denotes	]
T3062	3220-3222	,	denotes	,
T3063	3222-3227	IN	denotes	while
T3065	3228-3231	NN	denotes	BMP
T3067	3232-3236	NN	denotes	type
T3068	3237-3239	CD	denotes	II
T3066	3240-3248	NN	denotes	receptor
T3069	3249-3251	VBZ	denotes	is
T3064	3252-3258	VBN	denotes	needed
T3070	3259-3262	IN	denotes	for
T3071	3263-3269	JJ	denotes	proper
T3072	3270-3281	NN	denotes	development
T3073	3282-3284	IN	denotes	of
T3074	3285-3288	DT	denotes	the
T3076	3289-3300	JJ	denotes	conotruncal
T3075	3301-3307	NNS	denotes	ridges
T3077	3308-3309	-LRB-	denotes	[
T3078	3309-3311	CD	denotes	11
T3079	3311-3312	-RRB-	denotes	]
T3080	3312-3313	.	denotes	.
T3081	3313-3498	sentence	denotes	Moreover, neural crest-specific deletion of the BMP type I receptors Alk2 and Alk3 has been shown to lead to defective aortico-pulmonary septation, among other cardiac defects [12,13].
T3082	3314-3322	RB	denotes	Moreover
T3084	3322-3324	,	denotes	,
T3085	3324-3330	JJ	denotes	neural
T3086	3331-3336	NN	denotes	crest
T3088	3336-3337	HYPH	denotes	-
T3087	3337-3345	JJ	denotes	specific
T3089	3346-3354	NN	denotes	deletion
T3090	3355-3357	IN	denotes	of
T3091	3358-3361	DT	denotes	the
T3093	3362-3365	NN	denotes	BMP
T3094	3366-3370	NN	denotes	type
T3095	3371-3372	CD	denotes	I
T3096	3373-3382	NNS	denotes	receptors
T3092	3383-3387	NN	denotes	Alk2
T3097	3388-3391	CC	denotes	and
T3098	3392-3396	NN	denotes	Alk3
T3099	3397-3400	VBZ	denotes	has
T3100	3401-3405	VBN	denotes	been
T3083	3406-3411	VBN	denotes	shown
T3101	3412-3414	TO	denotes	to
T3102	3415-3419	VB	denotes	lead
T3103	3420-3422	IN	denotes	to
T3104	3423-3432	JJ	denotes	defective
T3106	3433-3440	JJ	denotes	aortico
T3108	3440-3441	HYPH	denotes	-
T3107	3441-3450	JJ	denotes	pulmonary
T3105	3451-3460	NN	denotes	septation
T3109	3460-3462	,	denotes	,
T3110	3462-3467	IN	denotes	among
T3111	3468-3473	JJ	denotes	other
T3113	3474-3481	JJ	denotes	cardiac
T3112	3482-3489	NNS	denotes	defects
T3114	3490-3491	-LRB-	denotes	[
T3116	3491-3493	CD	denotes	12
T3117	3493-3494	,	denotes	,
T3115	3494-3496	CD	denotes	13
T3118	3496-3497	-RRB-	denotes	]
T3119	3497-3498	.	denotes	.
T3120	3498-3620	sentence	denotes	TGF-β subfamily ligands signal via a receptor complex composed of two type II receptors and two type I receptors [14,15].
T3121	3499-3502	NN	denotes	TGF
T3123	3502-3503	HYPH	denotes	-
T3122	3503-3504	NN	denotes	β
T3125	3505-3514	NN	denotes	subfamily
T3124	3515-3522	NNS	denotes	ligands
T3126	3523-3529	VBP	denotes	signal
T3127	3530-3533	IN	denotes	via
T3128	3534-3535	DT	denotes	a
T3130	3536-3544	NN	denotes	receptor
T3129	3545-3552	NN	denotes	complex
T3131	3553-3561	VBN	denotes	composed
T3132	3562-3564	IN	denotes	of
T3133	3565-3568	CD	denotes	two
T3135	3569-3573	NN	denotes	type
T3136	3574-3576	CD	denotes	II
T3134	3577-3586	NNS	denotes	receptors
T3137	3587-3590	CC	denotes	and
T3138	3591-3594	CD	denotes	two
T3140	3595-3599	NN	denotes	type
T3141	3600-3601	CD	denotes	I
T3139	3602-3611	NNS	denotes	receptors
T3142	3612-3613	-LRB-	denotes	[
T3144	3613-3615	CD	denotes	14
T3145	3615-3616	,	denotes	,
T3143	3616-3618	CD	denotes	15
T3146	3618-3619	-RRB-	denotes	]
T3147	3619-3620	.	denotes	.
T3148	3620-3802	sentence	denotes	Ligand binding leads to phosphorylation and activation of type I receptors, which, in turn, phosphorylate and activate a specific set of downstream signaling molecules called Smads.
T3149	3621-3627	NN	denotes	Ligand
T3150	3628-3635	NN	denotes	binding
T3151	3636-3641	VBZ	denotes	leads
T3152	3642-3644	IN	denotes	to
T3153	3645-3660	NN	denotes	phosphorylation
T3154	3661-3664	CC	denotes	and
T3155	3665-3675	NN	denotes	activation
T3156	3676-3678	IN	denotes	of
T3157	3679-3683	NN	denotes	type
T3159	3684-3685	CD	denotes	I
T3158	3686-3695	NNS	denotes	receptors
T3160	3695-3697	,	denotes	,
T3161	3697-3702	WDT	denotes	which
T3163	3702-3704	,	denotes	,
T3164	3704-3706	IN	denotes	in
T3165	3707-3711	NN	denotes	turn
T3166	3711-3713	,	denotes	,
T3162	3713-3726	VBP	denotes	phosphorylate
T3167	3727-3730	CC	denotes	and
T3168	3731-3739	VBP	denotes	activate
T3169	3740-3741	DT	denotes	a
T3171	3742-3750	JJ	denotes	specific
T3170	3751-3754	NN	denotes	set
T3172	3755-3757	IN	denotes	of
T3173	3758-3768	JJ	denotes	downstream
T3175	3769-3778	NN	denotes	signaling
T3174	3779-3788	NNS	denotes	molecules
T3176	3789-3795	VBN	denotes	called
T3177	3796-3801	NNS	denotes	Smads
T3178	3801-3802	.	denotes	.
T3179	3802-4055	sentence	denotes	In general terms, TGF-βs bind to the TGF-β type II receptor (TGFβRII) and TGF-β type I receptor (ALK5) activating TGF-β Smads (2 and 3), while BMPs bind to the BMP type II receptor and type I receptors ALK2, -3, or 6, activating BMP Smads (1, 5 and 8).
T3180	3803-3805	IN	denotes	In
T3182	3806-3813	JJ	denotes	general
T3183	3814-3819	NNS	denotes	terms
T3184	3819-3821	,	denotes	,
T3185	3821-3824	NN	denotes	TGF
T3187	3824-3825	HYPH	denotes	-
T3186	3825-3827	NNS	denotes	βs
T3181	3828-3832	VBP	denotes	bind
T3188	3833-3835	IN	denotes	to
T3189	3836-3839	DT	denotes	the
T3191	3840-3843	NN	denotes	TGF
T3193	3843-3844	HYPH	denotes	-
T3192	3844-3845	NN	denotes	β
T3194	3846-3850	NN	denotes	type
T3195	3851-3853	CD	denotes	II
T3190	3854-3862	NN	denotes	receptor
T3196	3863-3864	-LRB-	denotes	(
T3197	3864-3871	NN	denotes	TGFβRII
T3198	3871-3872	-RRB-	denotes	)
T3199	3873-3876	CC	denotes	and
T3200	3877-3880	NN	denotes	TGF
T3202	3880-3881	HYPH	denotes	-
T3201	3881-3882	NN	denotes	β
T3204	3883-3887	NN	denotes	type
T3205	3888-3889	CD	denotes	I
T3203	3890-3898	NN	denotes	receptor
T3206	3899-3900	-LRB-	denotes	(
T3207	3900-3904	NN	denotes	ALK5
T3208	3904-3905	-RRB-	denotes	)
T3209	3906-3916	VBG	denotes	activating
T3210	3917-3920	NN	denotes	TGF
T3212	3920-3921	HYPH	denotes	-
T3211	3921-3922	NN	denotes	β
T3213	3923-3928	NNS	denotes	Smads
T3214	3929-3930	-LRB-	denotes	(
T3215	3930-3931	CD	denotes	2
T3216	3932-3935	CC	denotes	and
T3217	3936-3937	CD	denotes	3
T3218	3937-3938	-RRB-	denotes	)
T3219	3938-3940	,	denotes	,
T3220	3940-3945	IN	denotes	while
T3222	3946-3950	NNS	denotes	BMPs
T3221	3951-3955	VBP	denotes	bind
T3223	3956-3958	IN	denotes	to
T3224	3959-3962	DT	denotes	the
T3226	3963-3966	NN	denotes	BMP
T3227	3967-3971	NN	denotes	type
T3229	3972-3974	CD	denotes	II
T3228	3975-3983	NN	denotes	receptor
T3230	3984-3987	CC	denotes	and
T3231	3988-3992	NN	denotes	type
T3233	3993-3994	CD	denotes	I
T3232	3995-4004	NNS	denotes	receptors
T3225	4005-4009	NN	denotes	ALK2
T3234	4009-4011	,	denotes	,
T3235	4011-4012	SYM	denotes	-
T3236	4012-4013	CD	denotes	3
T3237	4013-4015	,	denotes	,
T3238	4015-4017	CC	denotes	or
T3239	4018-4019	CD	denotes	6
T3240	4019-4021	,	denotes	,
T3241	4021-4031	VBG	denotes	activating
T3242	4032-4035	NN	denotes	BMP
T3243	4036-4041	NNS	denotes	Smads
T3244	4042-4043	-LRB-	denotes	(
T3245	4043-4044	CD	denotes	1
T3246	4044-4046	,	denotes	,
T3247	4046-4047	CD	denotes	5
T3248	4048-4051	CC	denotes	and
T3249	4052-4053	CD	denotes	8
T3250	4053-4054	-RRB-	denotes	)
T3251	4054-4055	.	denotes	.
T3252	4055-4252	sentence	denotes	However, it is likely that these signaling interactions are more complex in vivo, possibly allowing formation of heterotetrameric complexes composed of different type II and type I receptors [16].
T3253	4056-4063	RB	denotes	However
T3255	4063-4065	,	denotes	,
T3256	4065-4067	PRP	denotes	it
T3254	4068-4070	VBZ	denotes	is
T3257	4071-4077	JJ	denotes	likely
T3258	4078-4082	IN	denotes	that
T3260	4083-4088	DT	denotes	these
T3262	4089-4098	NN	denotes	signaling
T3261	4099-4111	NNS	denotes	interactions
T3259	4112-4115	VBP	denotes	are
T3263	4116-4120	RBR	denotes	more
T3264	4121-4128	JJ	denotes	complex
T3265	4129-4131	FW	denotes	in
T3266	4132-4136	FW	denotes	vivo
T3267	4136-4138	,	denotes	,
T3268	4138-4146	RB	denotes	possibly
T3269	4147-4155	VBG	denotes	allowing
T3270	4156-4165	NN	denotes	formation
T3271	4166-4168	IN	denotes	of
T3272	4169-4185	JJ	denotes	heterotetrameric
T3273	4186-4195	NNS	denotes	complexes
T3274	4196-4204	VBN	denotes	composed
T3275	4205-4207	IN	denotes	of
T3276	4208-4217	JJ	denotes	different
T3278	4218-4222	NN	denotes	type
T3279	4223-4225	CD	denotes	II
T3280	4226-4229	CC	denotes	and
T3281	4230-4234	NN	denotes	type
T3282	4235-4236	CD	denotes	I
T3277	4237-4246	NNS	denotes	receptors
T3283	4247-4248	-LRB-	denotes	[
T3284	4248-4250	CD	denotes	16
T3285	4250-4251	-RRB-	denotes	]
T3286	4251-4252	.	denotes	.
T3287	4252-4387	sentence	denotes	In addition, recent studies have identified novel TGF-β-related ligands, which can bind to entire different combinations of receptors.
T3288	4253-4255	IN	denotes	In
T3290	4256-4264	NN	denotes	addition
T3291	4264-4266	,	denotes	,
T3292	4266-4272	JJ	denotes	recent
T3293	4273-4280	NNS	denotes	studies
T3294	4281-4285	VBP	denotes	have
T3289	4286-4296	VBN	denotes	identified
T3295	4297-4302	JJ	denotes	novel
T3297	4303-4306	NN	denotes	TGF
T3299	4306-4307	HYPH	denotes	-
T3298	4307-4308	NN	denotes	β
T3301	4308-4309	HYPH	denotes	-
T3300	4309-4316	VBN	denotes	related
T3296	4317-4324	NNS	denotes	ligands
T3302	4324-4326	,	denotes	,
T3303	4326-4331	WDT	denotes	which
T3305	4332-4335	MD	denotes	can
T3304	4336-4340	VB	denotes	bind
T3306	4341-4343	IN	denotes	to
T3307	4344-4350	JJ	denotes	entire
T3309	4351-4360	JJ	denotes	different
T3308	4361-4373	NNS	denotes	combinations
T3310	4374-4376	IN	denotes	of
T3311	4377-4386	NNS	denotes	receptors
T3312	4386-4387	.	denotes	.
T3313	4387-4530	sentence	denotes	For instance, growth and differentiation factors (GDFs) 8 and 9 can bind to Activin type II receptor and ALK5 to activate TGF-β Smads [17,18].
T3314	4388-4391	IN	denotes	For
T3316	4392-4400	NN	denotes	instance
T3317	4400-4402	,	denotes	,
T3318	4402-4408	NN	denotes	growth
T3320	4409-4412	CC	denotes	and
T3321	4413-4428	NN	denotes	differentiation
T3319	4429-4436	NNS	denotes	factors
T3322	4437-4438	-LRB-	denotes	(
T3323	4438-4442	NNS	denotes	GDFs
T3324	4442-4443	-RRB-	denotes	)
T3325	4444-4445	CD	denotes	8
T3326	4446-4449	CC	denotes	and
T3327	4450-4451	CD	denotes	9
T3328	4452-4455	MD	denotes	can
T3315	4456-4460	VB	denotes	bind
T3329	4461-4463	IN	denotes	to
T3330	4464-4471	NN	denotes	Activin
T3332	4472-4476	NN	denotes	type
T3333	4477-4479	CD	denotes	II
T3331	4480-4488	NN	denotes	receptor
T3334	4489-4492	CC	denotes	and
T3335	4493-4497	NN	denotes	ALK5
T3336	4498-4500	TO	denotes	to
T3337	4501-4509	VB	denotes	activate
T3338	4510-4513	NN	denotes	TGF
T3340	4513-4514	HYPH	denotes	-
T3339	4514-4515	NN	denotes	β
T3341	4516-4521	NNS	denotes	Smads
T3342	4522-4523	-LRB-	denotes	[
T3344	4523-4525	CD	denotes	17
T3345	4525-4526	,	denotes	,
T3343	4526-4528	CD	denotes	18
T3346	4528-4529	-RRB-	denotes	]
T3347	4529-4530	.	denotes	.
T3348	4530-4690	sentence	denotes	Therefore, we hypothesized that deletion of Alk5 in a specific cell lineage should reveal phenotypes which cannot be seen in comparable mutants lacking Tgfbr2.
T3349	4531-4540	RB	denotes	Therefore
T3351	4540-4542	,	denotes	,
T3352	4542-4544	PRP	denotes	we
T3350	4545-4557	VBD	denotes	hypothesized
T3353	4558-4562	IN	denotes	that
T3355	4563-4571	NN	denotes	deletion
T3356	4572-4574	IN	denotes	of
T3357	4575-4579	NN	denotes	Alk5
T3358	4580-4582	IN	denotes	in
T3359	4583-4584	DT	denotes	a
T3361	4585-4593	JJ	denotes	specific
T3362	4594-4598	NN	denotes	cell
T3360	4599-4606	NN	denotes	lineage
T3363	4607-4613	MD	denotes	should
T3354	4614-4620	VB	denotes	reveal
T3364	4621-4631	NNS	denotes	phenotypes
T3365	4632-4637	WDT	denotes	which
T3367	4638-4641	MD	denotes	can
T3368	4641-4644	RB	denotes	not
T3369	4645-4647	VB	denotes	be
T3366	4648-4652	VBN	denotes	seen
T3370	4653-4655	IN	denotes	in
T3371	4656-4666	JJ	denotes	comparable
T3372	4667-4674	NNS	denotes	mutants
T3373	4675-4682	VBG	denotes	lacking
T3374	4683-4689	NN	denotes	Tgfbr2
T3375	4689-4690	.	denotes	.
T3376	4690-4953	sentence	denotes	Indeed, we recently showed that neural crest cell specific Alk5 mutants display a unique spectrum of craniofacial developmental defects, e.g., cleft snout and severe mandibular hypoplasia [19]; these phenotypes were not seen in corresponding Tgfbr2 mutants [20].
T3377	4691-4697	RB	denotes	Indeed
T3379	4697-4699	,	denotes	,
T3380	4699-4701	PRP	denotes	we
T3381	4702-4710	RB	denotes	recently
T3378	4711-4717	VBD	denotes	showed
T3383	4718-4722	IN	denotes	that
T3385	4723-4729	JJ	denotes	neural
T3387	4730-4735	NN	denotes	crest
T3386	4736-4740	NN	denotes	cell
T3388	4741-4749	JJ	denotes	specific
T3390	4750-4754	NN	denotes	Alk5
T3389	4755-4762	NNS	denotes	mutants
T3384	4763-4770	VBP	denotes	display
T3391	4771-4772	DT	denotes	a
T3393	4773-4779	JJ	denotes	unique
T3392	4780-4788	NN	denotes	spectrum
T3394	4789-4791	IN	denotes	of
T3395	4792-4804	JJ	denotes	craniofacial
T3397	4805-4818	JJ	denotes	developmental
T3396	4819-4826	NNS	denotes	defects
T3398	4826-4828	,	denotes	,
T3399	4828-4832	FW	denotes	e.g.
T3401	4832-4834	,	denotes	,
T3402	4834-4839	NN	denotes	cleft
T3400	4840-4845	NN	denotes	snout
T3403	4846-4849	CC	denotes	and
T3404	4850-4856	JJ	denotes	severe
T3406	4857-4867	JJ	denotes	mandibular
T3405	4868-4878	NN	denotes	hypoplasia
T3407	4879-4880	-LRB-	denotes	[
T3408	4880-4882	CD	denotes	19
T3409	4882-4883	-RRB-	denotes	]
T3410	4883-4884	:	denotes	;
T3411	4885-4890	DT	denotes	these
T3412	4891-4901	NNS	denotes	phenotypes
T3413	4902-4906	VBD	denotes	were
T3414	4907-4910	RB	denotes	not
T3382	4911-4915	VBN	denotes	seen
T3415	4916-4918	IN	denotes	in
T3416	4919-4932	VBG	denotes	corresponding
T3418	4933-4939	NN	denotes	Tgfbr2
T3417	4940-4947	NNS	denotes	mutants
T3419	4948-4949	-LRB-	denotes	[
T3420	4949-4951	CD	denotes	20
T3421	4951-4952	-RRB-	denotes	]
T3422	4952-4953	.	denotes	.
T3423	4953-5182	sentence	denotes	To determine, whether ALK5 would also mediate unique non-redundant signaling events in cardiac neural crest cells, we focused on cardiac and pharyngeal phenotypes of mouse embryos lacking Alk5 specifically in neural crest cells.
T3424	4954-4956	TO	denotes	To
T3425	4957-4966	VB	denotes	determine
T3427	4966-4968	,	denotes	,
T3428	4968-4975	IN	denotes	whether
T3430	4976-4980	NN	denotes	ALK5
T3431	4981-4986	MD	denotes	would
T3432	4987-4991	RB	denotes	also
T3429	4992-4999	VB	denotes	mediate
T3433	5000-5006	JJ	denotes	unique
T3435	5007-5020	JJ	denotes	non-redundant
T3436	5021-5030	NN	denotes	signaling
T3434	5031-5037	NNS	denotes	events
T3437	5038-5040	IN	denotes	in
T3438	5041-5048	JJ	denotes	cardiac
T3440	5049-5055	JJ	denotes	neural
T3439	5056-5061	NN	denotes	crest
T3441	5062-5067	NNS	denotes	cells
T3442	5067-5069	,	denotes	,
T3443	5069-5071	PRP	denotes	we
T3426	5072-5079	VBD	denotes	focused
T3444	5080-5082	IN	denotes	on
T3445	5083-5090	JJ	denotes	cardiac
T3447	5091-5094	CC	denotes	and
T3448	5095-5105	JJ	denotes	pharyngeal
T3446	5106-5116	NNS	denotes	phenotypes
T3449	5117-5119	IN	denotes	of
T3450	5120-5125	NN	denotes	mouse
T3451	5126-5133	NNS	denotes	embryos
T3452	5134-5141	VBG	denotes	lacking
T3453	5142-5146	NN	denotes	Alk5
T3454	5147-5159	RB	denotes	specifically
T3455	5160-5162	IN	denotes	in
T3456	5163-5169	JJ	denotes	neural
T3458	5170-5175	NN	denotes	crest
T3457	5176-5181	NNS	denotes	cells
T3459	5181-5182	.	denotes	.
T3460	5182-5301	sentence	denotes	We discovered that in Alk5/Wnt1-Cre mutants, pharyngeal organs (thymus and parathyroid) fail to migrate appropriately.
T3461	5183-5185	PRP	denotes	We
T3462	5186-5196	VBD	denotes	discovered
T3463	5197-5201	IN	denotes	that
T3465	5202-5204	IN	denotes	in
T3466	5205-5209	NN	denotes	Alk5
T3468	5209-5210	HYPH	denotes	/
T3469	5210-5214	NN	denotes	Wnt1
T3470	5214-5215	HYPH	denotes	-
T3467	5215-5218	NN	denotes	Cre
T3471	5219-5226	NNS	denotes	mutants
T3472	5226-5228	,	denotes	,
T3473	5228-5238	JJ	denotes	pharyngeal
T3474	5239-5245	NNS	denotes	organs
T3475	5246-5247	-LRB-	denotes	(
T3476	5247-5253	NN	denotes	thymus
T3477	5254-5257	CC	denotes	and
T3478	5258-5269	NN	denotes	parathyroid
T3479	5269-5270	-RRB-	denotes	)
T3464	5271-5275	VBP	denotes	fail
T3480	5276-5278	TO	denotes	to
T3481	5279-5286	VB	denotes	migrate
T3482	5287-5300	RB	denotes	appropriately
T3483	5300-5301	.	denotes	.
T3484	5301-5447	sentence	denotes	Moreover, the mutant embryos display severe aortic sac and pharyngeal arch artery defects, and failed aortico-pulmonary septation leading to PTA.
T3485	5302-5310	RB	denotes	Moreover
T3487	5310-5312	,	denotes	,
T3488	5312-5315	DT	denotes	the
T3490	5316-5322	NN	denotes	mutant
T3489	5323-5330	NNS	denotes	embryos
T3486	5331-5338	VBP	denotes	display
T3491	5339-5345	JJ	denotes	severe
T3493	5346-5352	JJ	denotes	aortic
T3492	5353-5356	NN	denotes	sac
T3495	5357-5360	CC	denotes	and
T3496	5361-5371	JJ	denotes	pharyngeal
T3498	5372-5376	NN	denotes	arch
T3497	5377-5383	NN	denotes	artery
T3494	5384-5391	NNS	denotes	defects
T3499	5391-5393	,	denotes	,
T3500	5393-5396	CC	denotes	and
T3501	5397-5403	VBD	denotes	failed
T3502	5404-5411	JJ	denotes	aortico
T3504	5411-5412	HYPH	denotes	-
T3503	5412-5421	JJ	denotes	pulmonary
T3505	5422-5431	NN	denotes	septation
T3506	5432-5439	VBG	denotes	leading
T3507	5440-5442	IN	denotes	to
T3508	5443-5446	NN	denotes	PTA
T3509	5446-5447	.	denotes	.
T3510	5447-5619	sentence	denotes	Our data further suggest that at least some of these abnormal detected phenotypes result from a dramatic increase in apoptosis of postmigratory cardiac neural crest cells.
T3511	5448-5451	PRP$	denotes	Our
T3512	5452-5456	NNS	denotes	data
T3514	5457-5464	RB	denotes	further
T3513	5465-5472	VBP	denotes	suggest
T3515	5473-5477	IN	denotes	that
T3517	5478-5480	RB	denotes	at
T3518	5481-5486	RBS	denotes	least
T3519	5487-5491	DT	denotes	some
T3520	5492-5494	IN	denotes	of
T3521	5495-5500	DT	denotes	these
T3523	5501-5509	JJ	denotes	abnormal
T3524	5510-5518	VBN	denotes	detected
T3522	5519-5529	NNS	denotes	phenotypes
T3516	5530-5536	VBP	denotes	result
T3525	5537-5541	IN	denotes	from
T3526	5542-5543	DT	denotes	a
T3528	5544-5552	JJ	denotes	dramatic
T3527	5553-5561	NN	denotes	increase
T3529	5562-5564	IN	denotes	in
T3530	5565-5574	NN	denotes	apoptosis
T3531	5575-5577	IN	denotes	of
T3532	5578-5591	JJ	denotes	postmigratory
T3534	5592-5599	JJ	denotes	cardiac
T3536	5600-5606	JJ	denotes	neural
T3535	5607-5612	NN	denotes	crest
T3533	5613-5618	NNS	denotes	cells
T3537	5618-5619	.	denotes	.
T3538	5619-5886	sentence	denotes	These phenotypes differ remarkably from those seen in corresponding Tgfbr2 mutants, suggesting that ALK5 mediates a wider spectrum of signaling events than its classical binding partner TGFβRII in cardiac neural crest cells during cardiac and pharyngeal development.
T3539	5620-5625	DT	denotes	These
T3540	5626-5636	NNS	denotes	phenotypes
T3541	5637-5643	VBP	denotes	differ
T3542	5644-5654	RB	denotes	remarkably
T3543	5655-5659	IN	denotes	from
T3544	5660-5665	DT	denotes	those
T3545	5666-5670	VBN	denotes	seen
T3546	5671-5673	IN	denotes	in
T3547	5674-5687	VBG	denotes	corresponding
T3549	5688-5694	NN	denotes	Tgfbr2
T3548	5695-5702	NNS	denotes	mutants
T3550	5702-5704	,	denotes	,
T3551	5704-5714	VBG	denotes	suggesting
T3552	5715-5719	IN	denotes	that
T3554	5720-5724	NN	denotes	ALK5
T3553	5725-5733	VBZ	denotes	mediates
T3555	5734-5735	DT	denotes	a
T3557	5736-5741	JJR	denotes	wider
T3556	5742-5750	NN	denotes	spectrum
T3558	5751-5753	IN	denotes	of
T3559	5754-5763	NN	denotes	signaling
T3560	5764-5770	NNS	denotes	events
T3561	5771-5775	IN	denotes	than
T3562	5776-5779	PRP$	denotes	its
T3564	5780-5789	JJ	denotes	classical
T3565	5790-5797	NN	denotes	binding
T3563	5798-5805	NN	denotes	partner
T3566	5806-5813	NN	denotes	TGFβRII
T3567	5814-5816	IN	denotes	in
T3568	5817-5824	JJ	denotes	cardiac
T3570	5825-5831	JJ	denotes	neural
T3571	5832-5837	NN	denotes	crest
T3569	5838-5843	NNS	denotes	cells
T3572	5844-5850	IN	denotes	during
T3573	5851-5858	JJ	denotes	cardiac
T3575	5859-5862	CC	denotes	and
T3576	5863-5873	JJ	denotes	pharyngeal
T3574	5874-5885	NN	denotes	development
T3577	5885-5886	.	denotes	.
T4627	5897-5907	JJ	denotes	Persistent
T4629	5908-5915	NN	denotes	truncus
T4628	5916-5926	NN	denotes	arteriosus
T4630	5927-5930	CC	denotes	and
T4631	5931-5939	JJ	denotes	abnormal
T4633	5940-5945	JJ	denotes	large
T4632	5946-5953	NNS	denotes	vessels
T4634	5954-5956	IN	denotes	in
T4635	5957-5961	NNS	denotes	mice
T4636	5962-5969	VBG	denotes	lacking
T4637	5970-5974	NN	denotes	Alk5
T4638	5975-5977	IN	denotes	in
T4639	5978-5985	JJ	denotes	cardiac
T4640	5986-5990	NNS	denotes	NCCs
T4641	5990-6214	sentence	denotes	To inactivate Alk5 in cardiac NCCs, mice homozygous for the floxed Alk5 allele (Alk5Flox/Flox) [21] were crossed with transgenic Wnt1-Cre mice [22], which were also heterozygous for the Alk5 knockout allele (Alk5KO) allele.
T4642	5991-5993	TO	denotes	To
T4643	5994-6004	VB	denotes	inactivate
T4645	6005-6009	NN	denotes	Alk5
T4646	6010-6012	IN	denotes	in
T4647	6013-6020	JJ	denotes	cardiac
T4648	6021-6025	NNS	denotes	NCCs
T4649	6025-6027	,	denotes	,
T4650	6027-6031	NNS	denotes	mice
T4651	6032-6042	JJ	denotes	homozygous
T4652	6043-6046	IN	denotes	for
T4653	6047-6050	DT	denotes	the
T4655	6051-6057	VBN	denotes	floxed
T4656	6058-6062	NN	denotes	Alk5
T4654	6063-6069	NN	denotes	allele
T4657	6070-6071	-LRB-	denotes	(
T4658	6071-6079	NN	denotes	Alk5Flox
T4660	6079-6080	HYPH	denotes	/
T4659	6080-6084	NN	denotes	Flox
T4661	6084-6085	-RRB-	denotes	)
T4662	6086-6087	-LRB-	denotes	[
T4663	6087-6089	CD	denotes	21
T4664	6089-6090	-RRB-	denotes	]
T4665	6091-6095	VBD	denotes	were
T4644	6096-6103	VBN	denotes	crossed
T4666	6104-6108	IN	denotes	with
T4667	6109-6119	JJ	denotes	transgenic
T4669	6120-6124	NN	denotes	Wnt1
T4671	6124-6125	HYPH	denotes	-
T4670	6125-6128	NN	denotes	Cre
T4668	6129-6133	NNS	denotes	mice
T4672	6134-6135	-LRB-	denotes	[
T4673	6135-6137	CD	denotes	22
T4674	6137-6138	-RRB-	denotes	]
T4675	6138-6140	,	denotes	,
T4676	6140-6145	WDT	denotes	which
T4677	6146-6150	VBD	denotes	were
T4678	6151-6155	RB	denotes	also
T4679	6156-6168	JJ	denotes	heterozygous
T4680	6169-6172	IN	denotes	for
T4681	6173-6176	DT	denotes	the
T4683	6177-6181	NN	denotes	Alk5
T4684	6182-6190	NN	denotes	knockout
T4682	6191-6197	NN	denotes	allele
T4685	6198-6199	-LRB-	denotes	(
T4686	6199-6205	NN	denotes	Alk5KO
T4688	6205-6206	-RRB-	denotes	)
T4687	6207-6213	NN	denotes	allele
T4689	6213-6214	.	denotes	.
T4690	6214-6550	sentence	denotes	The resulting mice heterozygous for the Alk5Flox and Alk5KO alleles, which also carried the Wnt1-Cre transgene, had the Alk5 gene specifically inactivated in NCCs (herein termed Alk5/Wnt1-Cre), while the littermates with remaining allelic combinations were phenotypically normal and served as controls (Alk5Flox/+, Alk5KO/+; Wnt1-Cre).
T4691	6215-6218	DT	denotes	The
T4693	6219-6228	VBG	denotes	resulting
T4692	6229-6233	NNS	denotes	mice
T4695	6234-6246	JJ	denotes	heterozygous
T4696	6247-6250	IN	denotes	for
T4697	6251-6254	DT	denotes	the
T4699	6255-6263	NN	denotes	Alk5Flox
T4700	6264-6267	CC	denotes	and
T4701	6268-6274	NN	denotes	Alk5KO
T4698	6275-6282	NNS	denotes	alleles
T4702	6282-6284	,	denotes	,
T4703	6284-6289	WDT	denotes	which
T4705	6290-6294	RB	denotes	also
T4704	6295-6302	VBD	denotes	carried
T4706	6303-6306	DT	denotes	the
T4708	6307-6311	NN	denotes	Wnt1
T4710	6311-6312	HYPH	denotes	-
T4709	6312-6315	NN	denotes	Cre
T4707	6316-6325	NN	denotes	transgene
T4711	6325-6327	,	denotes	,
T4694	6327-6330	VBD	denotes	had
T4712	6331-6334	DT	denotes	the
T4714	6335-6339	NN	denotes	Alk5
T4713	6340-6344	NN	denotes	gene
T4715	6345-6357	RB	denotes	specifically
T4716	6358-6369	VBN	denotes	inactivated
T4717	6370-6372	IN	denotes	in
T4718	6373-6377	NNS	denotes	NCCs
T4719	6378-6379	-LRB-	denotes	(
T4720	6379-6385	RB	denotes	herein
T4721	6386-6392	VBN	denotes	termed
T4722	6393-6397	NN	denotes	Alk5
T4724	6397-6398	HYPH	denotes	/
T4725	6398-6402	NN	denotes	Wnt1
T4726	6402-6403	HYPH	denotes	-
T4723	6403-6406	NN	denotes	Cre
T4727	6406-6407	-RRB-	denotes	)
T4728	6407-6409	,	denotes	,
T4729	6409-6414	IN	denotes	while
T4731	6415-6418	DT	denotes	the
T4732	6419-6430	NNS	denotes	littermates
T4733	6431-6435	IN	denotes	with
T4734	6436-6445	VBG	denotes	remaining
T4736	6446-6453	JJ	denotes	allelic
T4735	6454-6466	NNS	denotes	combinations
T4730	6467-6471	VBD	denotes	were
T4737	6472-6486	RB	denotes	phenotypically
T4738	6487-6493	JJ	denotes	normal
T4739	6494-6497	CC	denotes	and
T4740	6498-6504	VBD	denotes	served
T4741	6505-6507	IN	denotes	as
T4742	6508-6516	NNS	denotes	controls
T4743	6517-6518	-LRB-	denotes	(
T4745	6518-6526	NN	denotes	Alk5Flox
T4746	6526-6527	HYPH	denotes	/
T4747	6527-6528	SYM	denotes	+
T4748	6528-6530	,	denotes	,
T4749	6530-6536	NN	denotes	Alk5KO
T4750	6536-6537	HYPH	denotes	/
T4751	6537-6538	SYM	denotes	+
T4752	6538-6539	:	denotes	;
T4753	6540-6544	NN	denotes	Wnt1
T4754	6544-6545	HYPH	denotes	-
T4744	6545-6548	NN	denotes	Cre
T4755	6548-6549	-RRB-	denotes	)
T4756	6549-6550	.	denotes	.
T4757	6550-6678	sentence	denotes	When embryos were harvested during the last day of gestation, an expected number (25%) of Alk5/Wnt1-Cre mutants were recovered.
T4758	6551-6555	WRB	denotes	When
T4760	6556-6563	NNS	denotes	embryos
T4761	6564-6568	VBD	denotes	were
T4759	6569-6578	VBN	denotes	harvested
T4763	6579-6585	IN	denotes	during
T4764	6586-6589	DT	denotes	the
T4766	6590-6594	JJ	denotes	last
T4765	6595-6598	NN	denotes	day
T4767	6599-6601	IN	denotes	of
T4768	6602-6611	NN	denotes	gestation
T4769	6611-6613	,	denotes	,
T4770	6613-6615	DT	denotes	an
T4772	6616-6624	JJ	denotes	expected
T4771	6625-6631	NN	denotes	number
T4773	6632-6633	-LRB-	denotes	(
T4774	6633-6635	CD	denotes	25
T4775	6635-6636	NN	denotes	%
T4776	6636-6637	-RRB-	denotes	)
T4777	6638-6640	IN	denotes	of
T4778	6641-6645	NN	denotes	Alk5
T4780	6645-6646	HYPH	denotes	/
T4781	6646-6650	NN	denotes	Wnt1
T4782	6650-6651	HYPH	denotes	-
T4779	6651-6654	NN	denotes	Cre
T4783	6655-6662	NNS	denotes	mutants
T4784	6663-6667	VBD	denotes	were
T4762	6668-6677	VBN	denotes	recovered
T4785	6677-6678	.	denotes	.
T4786	6678-6775	sentence	denotes	However, all mutant offspring died either during the birth or during the first post-natal hours.
T4787	6679-6686	RB	denotes	However
T4789	6686-6688	,	denotes	,
T4790	6688-6691	DT	denotes	all
T4792	6692-6698	NN	denotes	mutant
T4791	6699-6708	NN	denotes	offspring
T4788	6709-6713	VBD	denotes	died
T4793	6714-6720	CC	denotes	either
T4794	6721-6727	IN	denotes	during
T4795	6728-6731	DT	denotes	the
T4796	6732-6737	NN	denotes	birth
T4797	6738-6740	CC	denotes	or
T4798	6741-6747	IN	denotes	during
T4799	6748-6751	DT	denotes	the
T4801	6752-6757	JJ	denotes	first
T4802	6758-6768	JJ	denotes	post-natal
T4800	6769-6774	NNS	denotes	hours
T4803	6774-6775	.	denotes	.
T4804	6775-6962	sentence	denotes	To determine, if ALK5-mediated TGF-β-signaling had a role in development of the OFT and large vessels of the aortic arch, we performed casting dye experiments on E17 embryos (Fig. 1A–D).
T4805	6776-6778	TO	denotes	To
T4806	6779-6788	VB	denotes	determine
T4808	6788-6790	,	denotes	,
T4809	6790-6792	IN	denotes	if
T4811	6793-6797	NN	denotes	ALK5
T4813	6797-6798	HYPH	denotes	-
T4812	6798-6806	VBN	denotes	mediated
T4815	6807-6810	NN	denotes	TGF
T4817	6810-6811	HYPH	denotes	-
T4816	6811-6812	NN	denotes	β
T4818	6812-6813	HYPH	denotes	-
T4814	6813-6822	NN	denotes	signaling
T4810	6823-6826	VBD	denotes	had
T4819	6827-6828	DT	denotes	a
T4820	6829-6833	NN	denotes	role
T4821	6834-6836	IN	denotes	in
T4822	6837-6848	NN	denotes	development
T4823	6849-6851	IN	denotes	of
T4824	6852-6855	DT	denotes	the
T4825	6856-6859	NN	denotes	OFT
T4826	6860-6863	CC	denotes	and
T4827	6864-6869	JJ	denotes	large
T4828	6870-6877	NNS	denotes	vessels
T4829	6878-6880	IN	denotes	of
T4830	6881-6884	DT	denotes	the
T4832	6885-6891	JJ	denotes	aortic
T4831	6892-6896	NN	denotes	arch
T4833	6896-6898	,	denotes	,
T4834	6898-6900	PRP	denotes	we
T4807	6901-6910	VBD	denotes	performed
T4835	6911-6918	VBG	denotes	casting
T4836	6919-6922	NN	denotes	dye
T4837	6923-6934	NNS	denotes	experiments
T4838	6935-6937	IN	denotes	on
T4839	6938-6941	NN	denotes	E17
T4840	6942-6949	NNS	denotes	embryos
T4841	6950-6951	-LRB-	denotes	(
T4843	6951-6955	NN	denotes	Fig.
T4842	6956-6958	NN	denotes	1A
T4844	6958-6959	SYM	denotes	–
T4845	6959-6960	NN	denotes	D
T4846	6960-6961	-RRB-	denotes	)
T4847	6961-6962	.	denotes	.
T4848	6962-7164	sentence	denotes	In wild-type embryos (Fig. 1A), the aorta was clearly separated from the pulmonary trunk, and the right brachiocephalic, left carotid and left subclavian arteries branched directly off the aortic arch.
T4849	6963-6965	IN	denotes	In
T4851	6966-6970	JJ	denotes	wild
T4853	6970-6971	HYPH	denotes	-
T4852	6971-6975	NN	denotes	type
T4854	6976-6983	NNS	denotes	embryos
T4855	6984-6985	-LRB-	denotes	(
T4857	6985-6989	NN	denotes	Fig.
T4856	6990-6992	NN	denotes	1A
T4858	6992-6993	-RRB-	denotes	)
T4859	6993-6995	,	denotes	,
T4860	6995-6998	DT	denotes	the
T4861	6999-7004	NN	denotes	aorta
T4862	7005-7008	VBD	denotes	was
T4863	7009-7016	RB	denotes	clearly
T4850	7017-7026	VBN	denotes	separated
T4864	7027-7031	IN	denotes	from
T4865	7032-7035	DT	denotes	the
T4867	7036-7045	JJ	denotes	pulmonary
T4866	7046-7051	NN	denotes	trunk
T4868	7051-7053	,	denotes	,
T4869	7053-7056	CC	denotes	and
T4870	7057-7060	DT	denotes	the
T4872	7061-7066	JJ	denotes	right
T4873	7067-7082	JJ	denotes	brachiocephalic
T4874	7082-7084	,	denotes	,
T4875	7084-7088	JJ	denotes	left
T4876	7089-7096	NN	denotes	carotid
T4877	7097-7100	CC	denotes	and
T4878	7101-7105	JJ	denotes	left
T4879	7106-7116	JJ	denotes	subclavian
T4871	7117-7125	NNS	denotes	arteries
T4880	7126-7134	VBN	denotes	branched
T4881	7135-7143	RB	denotes	directly
T4882	7144-7147	IN	denotes	off
T4883	7148-7151	DT	denotes	the
T4885	7152-7158	JJ	denotes	aortic
T4884	7159-7163	NN	denotes	arch
T4886	7163-7164	.	denotes	.
T4887	7164-7387	sentence	denotes	In contrast, Alk5/Wnt1Cre mutant embryos consistently displayed a single prominent arterial trunk (Fig. 1C–D), while corresponding Tgfbr2 mutant embryos (Fig. 1B) displayed interrupted aortic arch, as reported earlier [8].
T4888	7165-7167	IN	denotes	In
T4890	7168-7176	NN	denotes	contrast
T4891	7176-7178	,	denotes	,
T4892	7178-7182	NN	denotes	Alk5
T4894	7182-7183	HYPH	denotes	/
T4893	7183-7190	NN	denotes	Wnt1Cre
T4896	7191-7197	NN	denotes	mutant
T4895	7198-7205	NNS	denotes	embryos
T4897	7206-7218	RB	denotes	consistently
T4889	7219-7228	VBD	denotes	displayed
T4898	7229-7230	DT	denotes	a
T4900	7231-7237	JJ	denotes	single
T4901	7238-7247	JJ	denotes	prominent
T4902	7248-7256	JJ	denotes	arterial
T4899	7257-7262	NN	denotes	trunk
T4903	7263-7264	-LRB-	denotes	(
T4905	7264-7268	NN	denotes	Fig.
T4904	7269-7271	NN	denotes	1C
T4906	7271-7272	SYM	denotes	–
T4907	7272-7273	NN	denotes	D
T4908	7273-7274	-RRB-	denotes	)
T4909	7274-7276	,	denotes	,
T4910	7276-7281	IN	denotes	while
T4912	7282-7295	VBG	denotes	corresponding
T4914	7296-7302	NN	denotes	Tgfbr2
T4915	7303-7309	NN	denotes	mutant
T4913	7310-7317	NNS	denotes	embryos
T4916	7318-7319	-LRB-	denotes	(
T4918	7319-7323	NN	denotes	Fig.
T4917	7324-7326	NN	denotes	1B
T4919	7326-7327	-RRB-	denotes	)
T4911	7328-7337	VBD	denotes	displayed
T4920	7338-7349	VBN	denotes	interrupted
T4922	7350-7356	JJ	denotes	aortic
T4921	7357-7361	NN	denotes	arch
T4923	7361-7363	,	denotes	,
T4924	7363-7365	IN	denotes	as
T4925	7366-7374	VBN	denotes	reported
T4926	7375-7382	RBR	denotes	earlier
T4927	7383-7384	-LRB-	denotes	[
T4928	7384-7385	CD	denotes	8
T4929	7385-7386	-RRB-	denotes	]
T4930	7386-7387	.	denotes	.
T4931	7387-7558	sentence	denotes	Approximately 40% of the Alk5 mutants had a right-sided outflow tract, with the retroesophageal arch connecting to the descending aorta and to the left subclavian artery.
T4932	7388-7401	RB	denotes	Approximately
T4933	7402-7404	CD	denotes	40
T4934	7404-7405	NN	denotes	%
T4936	7406-7408	IN	denotes	of
T4937	7409-7412	DT	denotes	the
T4939	7413-7417	NN	denotes	Alk5
T4938	7418-7425	NNS	denotes	mutants
T4935	7426-7429	VBD	denotes	had
T4940	7430-7431	DT	denotes	a
T4942	7432-7437	JJ	denotes	right
T4944	7437-7438	HYPH	denotes	-
T4943	7438-7443	VBN	denotes	sided
T4945	7444-7451	NN	denotes	outflow
T4941	7452-7457	NN	denotes	tract
T4946	7457-7459	,	denotes	,
T4947	7459-7463	IN	denotes	with
T4948	7464-7467	DT	denotes	the
T4950	7468-7483	JJ	denotes	retroesophageal
T4949	7484-7488	NN	denotes	arch
T4951	7489-7499	VBG	denotes	connecting
T4952	7500-7502	IN	denotes	to
T4953	7503-7506	DT	denotes	the
T4955	7507-7517	VBG	denotes	descending
T4954	7518-7523	NN	denotes	aorta
T4956	7524-7527	CC	denotes	and
T4957	7528-7530	IN	denotes	to
T4958	7531-7534	DT	denotes	the
T4960	7535-7539	JJ	denotes	left
T4961	7540-7550	JJ	denotes	subclavian
T4959	7551-7557	NN	denotes	artery
T4962	7557-7558	.	denotes	.
T4963	7558-7745	sentence	denotes	The carotid arteries originated either from a common bud located in the ventral side of the ascending arch, or from separate adjacent sites, as verified by serial sectioning (Fig. 1M–P).
T4964	7559-7562	DT	denotes	The
T4966	7563-7570	JJ	denotes	carotid
T4965	7571-7579	NNS	denotes	arteries
T4967	7580-7590	VBD	denotes	originated
T4968	7591-7597	CC	denotes	either
T4969	7598-7602	IN	denotes	from
T4970	7603-7604	DT	denotes	a
T4972	7605-7611	JJ	denotes	common
T4971	7612-7615	NN	denotes	bud
T4973	7616-7623	VBN	denotes	located
T4974	7624-7626	IN	denotes	in
T4975	7627-7630	DT	denotes	the
T4977	7631-7638	JJ	denotes	ventral
T4976	7639-7643	NN	denotes	side
T4978	7644-7646	IN	denotes	of
T4979	7647-7650	DT	denotes	the
T4981	7651-7660	VBG	denotes	ascending
T4980	7661-7665	NN	denotes	arch
T4982	7665-7667	,	denotes	,
T4983	7667-7669	CC	denotes	or
T4984	7670-7674	IN	denotes	from
T4985	7675-7683	JJ	denotes	separate
T4987	7684-7692	JJ	denotes	adjacent
T4986	7693-7698	NNS	denotes	sites
T4988	7698-7700	,	denotes	,
T4989	7700-7702	IN	denotes	as
T4990	7703-7711	VBN	denotes	verified
T4991	7712-7714	IN	denotes	by
T4992	7715-7721	JJ	denotes	serial
T4993	7722-7732	NN	denotes	sectioning
T4994	7733-7734	-LRB-	denotes	(
T4996	7734-7738	NN	denotes	Fig.
T4995	7739-7741	NN	denotes	1M
T4997	7741-7742	SYM	denotes	–
T4998	7742-7743	NN	denotes	P
T4999	7743-7744	-RRB-	denotes	)
T5000	7744-7745	.	denotes	.
T5001	7745-8008	sentence	denotes	The remaining mutants displayed a left-sided aortic arch, where the right carotid arteries originated from the right lateral aspect of the ascending trunk, while the left carotid arteries budded from the ventral or right ventral aspects of the trunk (Fig. 1I–L).
T5002	7746-7749	DT	denotes	The
T5004	7750-7759	VBG	denotes	remaining
T5003	7760-7767	NNS	denotes	mutants
T5005	7768-7777	VBD	denotes	displayed
T5006	7778-7779	DT	denotes	a
T5008	7780-7784	JJ	denotes	left
T5010	7784-7785	HYPH	denotes	-
T5009	7785-7790	VBN	denotes	sided
T5011	7791-7797	JJ	denotes	aortic
T5007	7798-7802	NN	denotes	arch
T5012	7802-7804	,	denotes	,
T5013	7804-7809	WRB	denotes	where
T5015	7810-7813	DT	denotes	the
T5017	7814-7819	JJ	denotes	right
T5018	7820-7827	NN	denotes	carotid
T5016	7828-7836	NNS	denotes	arteries
T5014	7837-7847	VBN	denotes	originated
T5019	7848-7852	IN	denotes	from
T5020	7853-7856	DT	denotes	the
T5022	7857-7862	JJ	denotes	right
T5023	7863-7870	JJ	denotes	lateral
T5021	7871-7877	NN	denotes	aspect
T5024	7878-7880	IN	denotes	of
T5025	7881-7884	DT	denotes	the
T5027	7885-7894	VBG	denotes	ascending
T5026	7895-7900	NN	denotes	trunk
T5028	7900-7902	,	denotes	,
T5029	7902-7907	IN	denotes	while
T5031	7908-7911	DT	denotes	the
T5033	7912-7916	JJ	denotes	left
T5034	7917-7924	NN	denotes	carotid
T5032	7925-7933	NNS	denotes	arteries
T5030	7934-7940	VBN	denotes	budded
T5035	7941-7945	IN	denotes	from
T5036	7946-7949	DT	denotes	the
T5038	7950-7957	JJ	denotes	ventral
T5039	7958-7960	CC	denotes	or
T5040	7961-7966	JJ	denotes	right
T5041	7967-7974	JJ	denotes	ventral
T5037	7975-7982	NNS	denotes	aspects
T5042	7983-7985	IN	denotes	of
T5043	7986-7989	DT	denotes	the
T5044	7990-7995	NN	denotes	trunk
T5045	7996-7997	-LRB-	denotes	(
T5047	7997-8001	NN	denotes	Fig.
T5046	8002-8004	NN	denotes	1I
T5048	8004-8005	SYM	denotes	–
T5049	8005-8006	NN	denotes	L
T5050	8006-8007	-RRB-	denotes	)
T5051	8007-8008	.	denotes	.
T5052	8008-8117	sentence	denotes	Both right and left subclavian arteries consistently originated from the descending part of the aortic arch.
T5053	8009-8013	CC	denotes	Both
T5055	8014-8019	JJ	denotes	right
T5056	8020-8023	CC	denotes	and
T5057	8024-8028	JJ	denotes	left
T5058	8029-8039	JJ	denotes	subclavian
T5054	8040-8048	NNS	denotes	arteries
T5060	8049-8061	RB	denotes	consistently
T5059	8062-8072	VBD	denotes	originated
T5061	8073-8077	IN	denotes	from
T5062	8078-8081	DT	denotes	the
T5064	8082-8092	VBG	denotes	descending
T5063	8093-8097	NN	denotes	part
T5065	8098-8100	IN	denotes	of
T5066	8101-8104	DT	denotes	the
T5068	8105-8111	JJ	denotes	aortic
T5067	8112-8116	NN	denotes	arch
T5069	8116-8117	.	denotes	.
T5070	8117-8234	sentence	denotes	Similarly, in all mutants both left and right pulmonary arteries always branched out from the common arterial trunk.
T5071	8118-8127	RB	denotes	Similarly
T5073	8127-8129	,	denotes	,
T5074	8129-8131	IN	denotes	in
T5075	8132-8135	DT	denotes	all
T5076	8136-8143	NNS	denotes	mutants
T5077	8144-8148	CC	denotes	both
T5078	8149-8153	JJ	denotes	left
T5080	8154-8157	CC	denotes	and
T5081	8158-8163	JJ	denotes	right
T5082	8164-8173	JJ	denotes	pulmonary
T5079	8174-8182	NNS	denotes	arteries
T5083	8183-8189	RB	denotes	always
T5072	8190-8198	VBD	denotes	branched
T5084	8199-8202	RP	denotes	out
T5085	8203-8207	IN	denotes	from
T5086	8208-8211	DT	denotes	the
T5088	8212-8218	JJ	denotes	common
T5089	8219-8227	JJ	denotes	arterial
T5087	8228-8233	NN	denotes	trunk
T5090	8233-8234	.	denotes	.
T5091	8234-8423	sentence	denotes	To conclude, Alk5/Wnt1-Cre mutants consistently displayed PTA, which differed significantly from the characteristic interrupted aortic arch phenotype seen in Tgfbr2/Wnt1-Cre mutants [8,9].
T5092	8235-8237	TO	denotes	To
T5093	8238-8246	VB	denotes	conclude
T5095	8246-8248	,	denotes	,
T5096	8248-8252	NN	denotes	Alk5
T5098	8252-8253	HYPH	denotes	/
T5099	8253-8257	NN	denotes	Wnt1
T5100	8257-8258	HYPH	denotes	-
T5097	8258-8261	NN	denotes	Cre
T5101	8262-8269	NNS	denotes	mutants
T5102	8270-8282	RB	denotes	consistently
T5094	8283-8292	VBD	denotes	displayed
T5103	8293-8296	NN	denotes	PTA
T5104	8296-8298	,	denotes	,
T5105	8298-8303	WDT	denotes	which
T5106	8304-8312	VBD	denotes	differed
T5107	8313-8326	RB	denotes	significantly
T5108	8327-8331	IN	denotes	from
T5109	8332-8335	DT	denotes	the
T5111	8336-8350	JJ	denotes	characteristic
T5112	8351-8362	VBN	denotes	interrupted
T5114	8363-8369	JJ	denotes	aortic
T5113	8370-8374	NN	denotes	arch
T5110	8375-8384	NN	denotes	phenotype
T5115	8385-8389	VBN	denotes	seen
T5116	8390-8392	IN	denotes	in
T5117	8393-8399	NN	denotes	Tgfbr2
T5119	8399-8400	HYPH	denotes	/
T5120	8400-8404	NN	denotes	Wnt1
T5121	8404-8405	HYPH	denotes	-
T5118	8405-8408	NN	denotes	Cre
T5122	8409-8416	NNS	denotes	mutants
T5123	8417-8418	-LRB-	denotes	[
T5125	8418-8419	CD	denotes	8
T5126	8419-8420	,	denotes	,
T5124	8420-8421	CD	denotes	9
T5127	8421-8422	-RRB-	denotes	]
T5128	8422-8423	.	denotes	.
T17691	8434-8444	NN	denotes	Abrogation
T17693	8445-8447	IN	denotes	of
T17694	8448-8452	NN	denotes	Alk5
T17695	8453-8455	IN	denotes	in
T17696	8456-8462	JJ	denotes	neural
T17698	8463-8468	NN	denotes	crest
T17697	8469-8474	NNS	denotes	cells
T17692	8475-8480	VBZ	denotes	leads
T17699	8481-8483	IN	denotes	to
T17700	8484-8494	JJ	denotes	persistent
T17702	8495-8502	NN	denotes	truncus
T17701	8503-8513	NN	denotes	arteriosus
T17703	8514-8518	NN	denotes	type
T17704	8519-8521	NN	denotes	A2
T17705	8521-8522	.	denotes	.
T17706	8522-8579	sentence	denotes	A-D, Casting-dye analysis of OFT morphogenesis at E17.0.
T17707	8523-8524	LS	denotes	A
T17709	8524-8525	SYM	denotes	-
T17710	8525-8526	LS	denotes	D
T17711	8526-8528	,	denotes	,
T17712	8528-8535	VBG	denotes	Casting
T17714	8535-8536	HYPH	denotes	-
T17713	8536-8539	NN	denotes	dye
T17708	8540-8548	NN	denotes	analysis
T17715	8549-8551	IN	denotes	of
T17716	8552-8555	NN	denotes	OFT
T17717	8556-8569	NN	denotes	morphogenesis
T17718	8570-8572	IN	denotes	at
T17719	8573-8578	NN	denotes	E17.0
T17720	8578-8579	.	denotes	.
T17721	8579-8812	sentence	denotes	Control (A), Tgfbr2/Wnt1-Cre mutant [8] (B) demonstrating the PTA type A4 (= truncus arteriosus with interrupted aortic arch [30]) and Alk5/Wnt1-Cre mutants demonstrating the right-sided (C) and left-sided (D) arches of the truncus.
T17722	8580-8587	NN	denotes	Control
T17724	8588-8589	-LRB-	denotes	(
T17725	8589-8590	NN	denotes	A
T17726	8590-8591	-RRB-	denotes	)
T17727	8591-8593	,	denotes	,
T17728	8593-8599	NN	denotes	Tgfbr2
T17730	8599-8600	HYPH	denotes	/
T17731	8600-8604	NN	denotes	Wnt1
T17732	8604-8605	HYPH	denotes	-
T17729	8605-8608	NN	denotes	Cre
T17723	8609-8615	NN	denotes	mutant
T17733	8616-8617	-LRB-	denotes	[
T17734	8617-8618	CD	denotes	8
T17735	8618-8619	-RRB-	denotes	]
T17736	8620-8621	-LRB-	denotes	(
T17737	8621-8622	NN	denotes	B
T17738	8622-8623	-RRB-	denotes	)
T17739	8624-8637	VBG	denotes	demonstrating
T17740	8638-8641	DT	denotes	the
T17742	8642-8645	NN	denotes	PTA
T17743	8646-8650	NN	denotes	type
T17741	8651-8653	NN	denotes	A4
T17744	8654-8655	-LRB-	denotes	(
T17745	8655-8656	SYM	denotes	=
T17747	8657-8664	NN	denotes	truncus
T17746	8665-8675	NN	denotes	arteriosus
T17748	8676-8680	IN	denotes	with
T17749	8681-8692	VBN	denotes	interrupted
T17751	8693-8699	JJ	denotes	aortic
T17750	8700-8704	NN	denotes	arch
T17752	8705-8706	-LRB-	denotes	[
T17753	8706-8708	CD	denotes	30
T17754	8708-8709	-RRB-	denotes	]
T17755	8709-8710	-RRB-	denotes	)
T17756	8711-8714	CC	denotes	and
T17757	8715-8719	NN	denotes	Alk5
T17759	8719-8720	HYPH	denotes	/
T17760	8720-8724	NN	denotes	Wnt1
T17761	8724-8725	HYPH	denotes	-
T17758	8725-8728	NN	denotes	Cre
T17762	8729-8736	NNS	denotes	mutants
T17763	8737-8750	VBG	denotes	demonstrating
T17764	8751-8754	DT	denotes	the
T17766	8755-8760	JJ	denotes	right
T17768	8760-8761	HYPH	denotes	-
T17767	8761-8766	VBN	denotes	sided
T17769	8767-8768	-LRB-	denotes	(
T17770	8768-8769	NN	denotes	C
T17771	8769-8770	-RRB-	denotes	)
T17772	8771-8774	CC	denotes	and
T17773	8775-8779	JJ	denotes	left
T17775	8779-8780	HYPH	denotes	-
T17774	8780-8785	VBN	denotes	sided
T17776	8786-8787	-LRB-	denotes	(
T17777	8787-8788	NN	denotes	D
T17778	8788-8789	-RRB-	denotes	)
T17765	8790-8796	NNS	denotes	arches
T17779	8797-8799	IN	denotes	of
T17780	8800-8803	DT	denotes	the
T17781	8804-8811	NN	denotes	truncus
T17782	8811-8812	.	denotes	.
T17783	8812-8900	sentence	denotes	E-P, Histological cross-sections on four different levels (rostral to caudal) at E17.0.
T17784	8813-8814	LS	denotes	E
T17786	8814-8815	SYM	denotes	-
T17787	8815-8816	LS	denotes	P
T17788	8816-8818	,	denotes	,
T17789	8818-8830	JJ	denotes	Histological
T17785	8831-8845	NNS	denotes	cross-sections
T17790	8846-8848	IN	denotes	on
T17791	8849-8853	CD	denotes	four
T17793	8854-8863	JJ	denotes	different
T17792	8864-8870	NNS	denotes	levels
T17794	8871-8872	-LRB-	denotes	(
T17795	8872-8879	JJ	denotes	rostral
T17796	8880-8882	IN	denotes	to
T17797	8883-8889	JJ	denotes	caudal
T17798	8889-8890	-RRB-	denotes	)
T17799	8891-8893	IN	denotes	at
T17800	8894-8899	NN	denotes	E17.0
T17801	8899-8900	.	denotes	.
T17802	8900-9011	sentence	denotes	In a control (E-H), the ascending aorta (Ao) and pulmonary trunk (PT) are separated by the conotruncal septum.
T17803	8901-8903	IN	denotes	In
T17805	8904-8905	DT	denotes	a
T17806	8906-8913	NN	denotes	control
T17807	8914-8915	-LRB-	denotes	(
T17808	8915-8916	NN	denotes	E
T17809	8916-8917	SYM	denotes	-
T17810	8917-8918	NN	denotes	H
T17811	8918-8919	-RRB-	denotes	)
T17812	8919-8921	,	denotes	,
T17813	8921-8924	DT	denotes	the
T17815	8925-8934	VBG	denotes	ascending
T17814	8935-8940	NN	denotes	aorta
T17816	8941-8942	-LRB-	denotes	(
T17817	8942-8944	NN	denotes	Ao
T17818	8944-8945	-RRB-	denotes	)
T17819	8946-8949	CC	denotes	and
T17820	8950-8959	JJ	denotes	pulmonary
T17821	8960-8965	NN	denotes	trunk
T17822	8966-8967	-LRB-	denotes	(
T17823	8967-8969	NN	denotes	PT
T17824	8969-8970	-RRB-	denotes	)
T17825	8971-8974	VBP	denotes	are
T17804	8975-8984	VBN	denotes	separated
T17826	8985-8987	IN	denotes	by
T17827	8988-8991	DT	denotes	the
T17829	8992-9003	JJ	denotes	conotruncal
T17828	9004-9010	NN	denotes	septum
T17830	9010-9011	.	denotes	.
T17831	9011-9154	sentence	denotes	In Alk5/Wnt1-Cre mutants (I-P) the conotruncal septum fails to form, and either left-sided (I-L) or right-sided (M-P) aortic arch can be seen.
T17832	9012-9014	IN	denotes	In
T17834	9015-9019	NN	denotes	Alk5
T17836	9019-9020	HYPH	denotes	/
T17837	9020-9024	NN	denotes	Wnt1
T17838	9024-9025	HYPH	denotes	-
T17835	9025-9028	NN	denotes	Cre
T17839	9029-9036	NNS	denotes	mutants
T17840	9037-9038	-LRB-	denotes	(
T17841	9038-9039	NN	denotes	I
T17842	9039-9040	SYM	denotes	-
T17843	9040-9041	NN	denotes	P
T17844	9041-9042	-RRB-	denotes	)
T17845	9043-9046	DT	denotes	the
T17847	9047-9058	JJ	denotes	conotruncal
T17846	9059-9065	NN	denotes	septum
T17833	9066-9071	VBZ	denotes	fails
T17848	9072-9074	TO	denotes	to
T17849	9075-9079	VB	denotes	form
T17850	9079-9081	,	denotes	,
T17851	9081-9084	CC	denotes	and
T17852	9085-9091	CC	denotes	either
T17854	9092-9096	JJ	denotes	left
T17855	9096-9097	HYPH	denotes	-
T17853	9097-9102	VBN	denotes	sided
T17857	9103-9104	-LRB-	denotes	(
T17858	9104-9105	NN	denotes	I
T17859	9105-9106	SYM	denotes	-
T17860	9106-9107	NN	denotes	L
T17861	9107-9108	-RRB-	denotes	)
T17862	9109-9111	CC	denotes	or
T17863	9112-9117	JJ	denotes	right
T17865	9117-9118	HYPH	denotes	-
T17864	9118-9123	VBN	denotes	sided
T17866	9124-9125	-LRB-	denotes	(
T17867	9125-9126	NN	denotes	M
T17868	9126-9127	SYM	denotes	-
T17869	9127-9128	NN	denotes	P
T17870	9128-9129	-RRB-	denotes	)
T17871	9130-9136	JJ	denotes	aortic
T17856	9137-9141	NN	denotes	arch
T17873	9142-9145	MD	denotes	can
T17874	9146-9148	VB	denotes	be
T17872	9149-9153	VBN	denotes	seen
T17875	9153-9154	.	denotes	.
T17876	9154-9258	sentence	denotes	Aberrant branching of carotid arteries from the truncus has been illustrated by black arrows (J and M).
T17877	9155-9163	JJ	denotes	Aberrant
T17878	9164-9173	NN	denotes	branching
T17880	9174-9176	IN	denotes	of
T17881	9177-9184	JJ	denotes	carotid
T17882	9185-9193	NNS	denotes	arteries
T17883	9194-9198	IN	denotes	from
T17884	9199-9202	DT	denotes	the
T17885	9203-9210	NN	denotes	truncus
T17886	9211-9214	VBZ	denotes	has
T17887	9215-9219	VBN	denotes	been
T17879	9220-9231	VBN	denotes	illustrated
T17888	9232-9234	IN	denotes	by
T17889	9235-9240	JJ	denotes	black
T17890	9241-9247	NNS	denotes	arrows
T17891	9248-9249	-LRB-	denotes	(
T17892	9249-9250	NN	denotes	J
T17893	9251-9254	CC	denotes	and
T17894	9255-9256	NN	denotes	M
T17895	9256-9257	-RRB-	denotes	)
T17896	9257-9258	.	denotes	.
T17897	9258-9468	sentence	denotes	Ao, aorta; PT, pulmonary trunk; RSA, right subclavian artery; RCA, right carotid artery; LCA; left carotid artery; LSA, left subclavian artery; IAA, interrupted aortic arch; PTA, persistent truncus arteriosus.
T17898	9259-9261	NN	denotes	Ao
T17899	9261-9263	,	denotes	,
T17900	9263-9268	NN	denotes	aorta
T17901	9268-9269	:	denotes	;
T17902	9270-9272	NN	denotes	PT
T17903	9272-9274	,	denotes	,
T17904	9274-9283	JJ	denotes	pulmonary
T17905	9284-9289	NN	denotes	trunk
T17906	9289-9290	:	denotes	;
T17907	9291-9294	NN	denotes	RSA
T17908	9294-9296	,	denotes	,
T17909	9296-9301	JJ	denotes	right
T17911	9302-9312	JJ	denotes	subclavian
T17910	9313-9319	NN	denotes	artery
T17912	9319-9320	:	denotes	;
T17913	9321-9324	NN	denotes	RCA
T17914	9324-9326	,	denotes	,
T17915	9326-9331	JJ	denotes	right
T17917	9332-9339	NN	denotes	carotid
T17916	9340-9346	NN	denotes	artery
T17918	9346-9347	:	denotes	;
T17919	9348-9351	NN	denotes	LCA
T17920	9351-9352	:	denotes	;
T17921	9353-9357	JJ	denotes	left
T17923	9358-9365	JJ	denotes	carotid
T17922	9366-9372	NN	denotes	artery
T17924	9372-9373	:	denotes	;
T17925	9374-9377	NN	denotes	LSA
T17926	9377-9379	,	denotes	,
T17927	9379-9383	JJ	denotes	left
T17929	9384-9394	JJ	denotes	subclavian
T17928	9395-9401	NN	denotes	artery
T17930	9401-9402	:	denotes	;
T17931	9403-9406	NN	denotes	IAA
T17932	9406-9408	,	denotes	,
T17933	9408-9419	VBN	denotes	interrupted
T17935	9420-9426	JJ	denotes	aortic
T17934	9427-9431	NN	denotes	arch
T17936	9431-9432	:	denotes	;
T17937	9433-9436	NN	denotes	PTA
T17938	9436-9438	,	denotes	,
T17939	9438-9448	JJ	denotes	persistent
T17941	9449-9456	NN	denotes	truncus
T17940	9457-9467	NN	denotes	arteriosus
T17942	9467-9468	.	denotes	.
T5938	9470-9478	JJ	denotes	Abnormal
T5939	9479-9489	NN	denotes	patterning
T5940	9490-9492	IN	denotes	of
T5941	9493-9496	DT	denotes	the
T5943	9497-9507	JJ	denotes	pharyngeal
T5944	9508-9512	NN	denotes	arch
T5942	9513-9521	NNS	denotes	arteries
T5945	9522-9525	CC	denotes	and
T5946	9526-9532	JJ	denotes	aortic
T5947	9533-9536	NN	denotes	sac
T5948	9537-9539	IN	denotes	in
T5949	9540-9544	NN	denotes	Alk5
T5951	9544-9545	HYPH	denotes	/
T5950	9545-9552	NN	denotes	Wnt1Cre
T5952	9553-9560	NNS	denotes	mutants
T5953	9560-9709	sentence	denotes	During cardiovascular development, the PAAs undergo a complex set of sequential asymmetric remodeling steps resulting in the left-sided aortic arch.
T5954	9561-9567	IN	denotes	During
T5956	9568-9582	JJ	denotes	cardiovascular
T5957	9583-9594	NN	denotes	development
T5958	9594-9596	,	denotes	,
T5959	9596-9599	DT	denotes	the
T5960	9600-9604	NNS	denotes	PAAs
T5955	9605-9612	VBP	denotes	undergo
T5961	9613-9614	DT	denotes	a
T5963	9615-9622	JJ	denotes	complex
T5962	9623-9626	NN	denotes	set
T5964	9627-9629	IN	denotes	of
T5965	9630-9640	JJ	denotes	sequential
T5967	9641-9651	JJ	denotes	asymmetric
T5968	9652-9662	NN	denotes	remodeling
T5966	9663-9668	NNS	denotes	steps
T5969	9669-9678	VBG	denotes	resulting
T5970	9679-9681	IN	denotes	in
T5971	9682-9685	DT	denotes	the
T5973	9686-9690	JJ	denotes	left
T5975	9690-9691	HYPH	denotes	-
T5974	9691-9696	VBN	denotes	sided
T5976	9697-9703	JJ	denotes	aortic
T5972	9704-9708	NN	denotes	arch
T5977	9708-9709	.	denotes	.
T5978	9709-9873	sentence	denotes	To determine, whether ALK5-mediated signaling was involved in remodeling of PAAs, we performed intracardiac India ink injections at different developmental stages.
T5979	9710-9712	TO	denotes	To
T5980	9713-9722	VB	denotes	determine
T5982	9722-9724	,	denotes	,
T5983	9724-9731	IN	denotes	whether
T5985	9732-9736	NN	denotes	ALK5
T5987	9736-9737	HYPH	denotes	-
T5986	9737-9745	VBN	denotes	mediated
T5988	9746-9755	NN	denotes	signaling
T5989	9756-9759	VBD	denotes	was
T5984	9760-9768	VBN	denotes	involved
T5990	9769-9771	IN	denotes	in
T5991	9772-9782	NN	denotes	remodeling
T5992	9783-9785	IN	denotes	of
T5993	9786-9790	NNS	denotes	PAAs
T5994	9790-9792	,	denotes	,
T5995	9792-9794	PRP	denotes	we
T5981	9795-9804	VBD	denotes	performed
T5996	9805-9817	JJ	denotes	intracardiac
T5998	9818-9823	NNP	denotes	India
T5999	9824-9827	NN	denotes	ink
T5997	9828-9838	NNS	denotes	injections
T6000	9839-9841	IN	denotes	at
T6001	9842-9851	JJ	denotes	different
T6003	9852-9865	JJ	denotes	developmental
T6002	9866-9872	NNS	denotes	stages
T6004	9872-9873	.	denotes	.
T6005	9873-10030	sentence	denotes	While at E10, Alk5/Wnt1-Cre mutants did not show obvious differences in the PAAs, abnormal remodeling became obvious in mutants a day later at E11 (Fig. 2).
T6006	9874-9879	IN	denotes	While
T6008	9880-9882	IN	denotes	at
T6009	9883-9886	NN	denotes	E10
T6010	9886-9888	,	denotes	,
T6011	9888-9892	NN	denotes	Alk5
T6013	9892-9893	HYPH	denotes	/
T6014	9893-9897	NN	denotes	Wnt1
T6015	9897-9898	HYPH	denotes	-
T6012	9898-9901	NN	denotes	Cre
T6016	9902-9909	NNS	denotes	mutants
T6017	9910-9913	VBD	denotes	did
T6018	9914-9917	RB	denotes	not
T6007	9918-9922	VB	denotes	show
T6020	9923-9930	JJ	denotes	obvious
T6021	9931-9942	NNS	denotes	differences
T6022	9943-9945	IN	denotes	in
T6023	9946-9949	DT	denotes	the
T6024	9950-9954	NNS	denotes	PAAs
T6025	9954-9956	,	denotes	,
T6026	9956-9964	JJ	denotes	abnormal
T6027	9965-9975	NN	denotes	remodeling
T6019	9976-9982	VBD	denotes	became
T6028	9983-9990	JJ	denotes	obvious
T6029	9991-9993	IN	denotes	in
T6030	9994-10001	NNS	denotes	mutants
T6031	10002-10003	DT	denotes	a
T6032	10004-10007	NN	denotes	day
T6033	10008-10013	RB	denotes	later
T6034	10014-10016	IN	denotes	at
T6035	10017-10020	NN	denotes	E11
T6036	10021-10022	-LRB-	denotes	(
T6037	10022-10026	NN	denotes	Fig.
T6038	10027-10028	CD	denotes	2
T6039	10028-10029	-RRB-	denotes	)
T6040	10029-10030	.	denotes	.
T6041	10030-10092	sentence	denotes	The controls displayed the well-formed 3rd, 4th and 6th PAAs.
T6042	10031-10034	DT	denotes	The
T6043	10035-10043	NNS	denotes	controls
T6044	10044-10053	VBD	denotes	displayed
T6045	10054-10057	DT	denotes	the
T6047	10058-10062	RB	denotes	well
T6049	10062-10063	HYPH	denotes	-
T6048	10063-10069	VBN	denotes	formed
T6050	10070-10073	JJ	denotes	3rd
T6051	10073-10075	,	denotes	,
T6052	10075-10078	JJ	denotes	4th
T6053	10079-10082	CC	denotes	and
T6054	10083-10086	JJ	denotes	6th
T6046	10087-10091	NNS	denotes	PAAs
T6055	10091-10092	.	denotes	.
T6056	10092-10237	sentence	denotes	Moreover, the carotid duct (the dorsal aorta between the 3rd and 4th PAAs) was already regressing as demonstrated by the reduced size (Fig. 2A).
T6057	10093-10101	RB	denotes	Moreover
T6059	10101-10103	,	denotes	,
T6060	10103-10106	DT	denotes	the
T6062	10107-10114	JJ	denotes	carotid
T6061	10115-10119	NN	denotes	duct
T6063	10120-10121	-LRB-	denotes	(
T6064	10121-10124	DT	denotes	the
T6066	10125-10131	JJ	denotes	dorsal
T6065	10132-10137	NN	denotes	aorta
T6067	10138-10145	IN	denotes	between
T6068	10146-10149	DT	denotes	the
T6070	10150-10153	JJ	denotes	3rd
T6071	10154-10157	CC	denotes	and
T6072	10158-10161	JJ	denotes	4th
T6069	10162-10166	NNS	denotes	PAAs
T6073	10166-10167	-RRB-	denotes	)
T6074	10168-10171	VBD	denotes	was
T6075	10172-10179	RB	denotes	already
T6058	10180-10190	VBG	denotes	regressing
T6076	10191-10193	IN	denotes	as
T6077	10194-10206	VBN	denotes	demonstrated
T6078	10207-10209	IN	denotes	by
T6079	10210-10213	DT	denotes	the
T6081	10214-10221	VBN	denotes	reduced
T6080	10222-10226	NN	denotes	size
T6082	10227-10228	-LRB-	denotes	(
T6084	10228-10232	NN	denotes	Fig.
T6083	10233-10235	NN	denotes	2A
T6085	10235-10236	-RRB-	denotes	)
T6086	10236-10237	.	denotes	.
T6087	10237-10388	sentence	denotes	In Alk5/Wnt1-Cre mutants, the 3rd and 4th pairs of PAAs were bilaterally hypoplastic, whereas the 6th pair of PAAs was notably hyperplastic (Fig. 2B).
T6088	10238-10240	IN	denotes	In
T6090	10241-10245	NN	denotes	Alk5
T6092	10245-10246	HYPH	denotes	/
T6093	10246-10250	NN	denotes	Wnt1
T6094	10250-10251	HYPH	denotes	-
T6091	10251-10254	NN	denotes	Cre
T6095	10255-10262	NNS	denotes	mutants
T6096	10262-10264	,	denotes	,
T6097	10264-10267	DT	denotes	the
T6099	10268-10271	JJ	denotes	3rd
T6100	10272-10275	CC	denotes	and
T6101	10276-10279	JJ	denotes	4th
T6098	10280-10285	NNS	denotes	pairs
T6102	10286-10288	IN	denotes	of
T6103	10289-10293	NNS	denotes	PAAs
T6089	10294-10298	VBD	denotes	were
T6104	10299-10310	RB	denotes	bilaterally
T6105	10311-10322	JJ	denotes	hypoplastic
T6106	10322-10324	,	denotes	,
T6107	10324-10331	IN	denotes	whereas
T6109	10332-10335	DT	denotes	the
T6111	10336-10339	JJ	denotes	6th
T6110	10340-10344	NN	denotes	pair
T6112	10345-10347	IN	denotes	of
T6113	10348-10352	NNS	denotes	PAAs
T6108	10353-10356	VBD	denotes	was
T6114	10357-10364	RB	denotes	notably
T6115	10365-10377	JJ	denotes	hyperplastic
T6116	10378-10379	-LRB-	denotes	(
T6118	10379-10383	NN	denotes	Fig.
T6117	10384-10386	NN	denotes	2B
T6119	10386-10387	-RRB-	denotes	)
T6120	10387-10388	.	denotes	.
T6121	10388-10467	sentence	denotes	Furthermore, the carotid duct was remarkably large, when compared to controls.
T6122	10389-10400	RB	denotes	Furthermore
T6124	10400-10402	,	denotes	,
T6125	10402-10405	DT	denotes	the
T6127	10406-10413	JJ	denotes	carotid
T6126	10414-10418	NN	denotes	duct
T6123	10419-10422	VBD	denotes	was
T6128	10423-10433	RB	denotes	remarkably
T6129	10434-10439	JJ	denotes	large
T6130	10439-10441	,	denotes	,
T6131	10441-10445	WRB	denotes	when
T6132	10446-10454	VBN	denotes	compared
T6133	10455-10457	IN	denotes	to
T6134	10458-10466	NNS	denotes	controls
T6135	10466-10467	.	denotes	.
T6136	10467-10690	sentence	denotes	While the controls displayed an interruption of the carotid duct at E12 and E13 as expected (Fig. 2C), the mutants demonstrated an uncharacteristic break of the dorsal aorta between the 4th and 6th pairs of PAAs (Fig. 2D).
T6137	10468-10473	IN	denotes	While
T6139	10474-10477	DT	denotes	the
T6140	10478-10486	NNS	denotes	controls
T6138	10487-10496	VBD	denotes	displayed
T6142	10497-10499	DT	denotes	an
T6143	10500-10512	NN	denotes	interruption
T6144	10513-10515	IN	denotes	of
T6145	10516-10519	DT	denotes	the
T6147	10520-10527	JJ	denotes	carotid
T6146	10528-10532	NN	denotes	duct
T6148	10533-10535	IN	denotes	at
T6149	10536-10539	NN	denotes	E12
T6150	10540-10543	CC	denotes	and
T6151	10544-10547	NN	denotes	E13
T6152	10548-10550	IN	denotes	as
T6153	10551-10559	VBN	denotes	expected
T6154	10560-10561	-LRB-	denotes	(
T6156	10561-10565	NN	denotes	Fig.
T6155	10566-10568	NN	denotes	2C
T6157	10568-10569	-RRB-	denotes	)
T6158	10569-10571	,	denotes	,
T6159	10571-10574	DT	denotes	the
T6160	10575-10582	NNS	denotes	mutants
T6141	10583-10595	VBD	denotes	demonstrated
T6161	10596-10598	DT	denotes	an
T6163	10599-10615	JJ	denotes	uncharacteristic
T6162	10616-10621	NN	denotes	break
T6164	10622-10624	IN	denotes	of
T6165	10625-10628	DT	denotes	the
T6167	10629-10635	JJ	denotes	dorsal
T6166	10636-10641	NN	denotes	aorta
T6168	10642-10649	IN	denotes	between
T6169	10650-10653	DT	denotes	the
T6171	10654-10657	JJ	denotes	4th
T6172	10658-10661	CC	denotes	and
T6173	10662-10665	JJ	denotes	6th
T6170	10666-10671	NNS	denotes	pairs
T6174	10672-10674	IN	denotes	of
T6175	10675-10679	NNS	denotes	PAAs
T6176	10680-10681	-LRB-	denotes	(
T6178	10681-10685	NN	denotes	Fig.
T6177	10686-10688	NN	denotes	2D
T6179	10688-10689	-RRB-	denotes	)
T6180	10689-10690	.	denotes	.
T6181	10690-11391	sentence	denotes	Figure 2 Abnormal patterning of the PAAs in Alk5/Wnt1-Cre mutants. Left lateral view after intracardiac ink injections to visualize the developing PAAs at E11.0 (A,B), E12.0 (C, D) and E13.0 (E, F) in controls (A, C, E) and Alk5/Wnt1-Cre mutants (B, D, F). Arrow in A points to the regressing carotid duct. Asterisk in B depicts the corresponding structure in the mutant with no signs of regression. Asterisk in D illustrates the aberrant regression of the dorsal aorta between the 4th and 6th PAAs. PT, pulmonary trunk; Ao, Aorta; TA, truncus arteriosus. Around E11.5, the aortic sac normally forms a distinctive T-shaped structure, as seen in frontal sections of the control sample in Fig. 3(A,C).
T18219	10701-10709	JJ	denotes	Abnormal
T18220	10710-10720	NN	denotes	patterning
T18221	10721-10723	IN	denotes	of
T18222	10724-10727	DT	denotes	the
T18223	10728-10732	NNS	denotes	PAAs
T18224	10733-10735	IN	denotes	in
T18225	10736-10740	NN	denotes	Alk5
T18227	10740-10741	HYPH	denotes	/
T18228	10741-10745	NN	denotes	Wnt1
T18229	10745-10746	HYPH	denotes	-
T18226	10746-10749	NN	denotes	Cre
T18230	10750-10757	NNS	denotes	mutants
T18231	10757-10758	.	denotes	.
T18232	10758-10948	sentence	denotes	Left lateral view after intracardiac ink injections to visualize the developing PAAs at E11.0 (A,B), E12.0 (C, D) and E13.0 (E, F) in controls (A, C, E) and Alk5/Wnt1-Cre mutants (B, D, F).
T18233	10759-10763	JJ	denotes	Left
T18235	10764-10771	JJ	denotes	lateral
T18234	10772-10776	NN	denotes	view
T18236	10777-10782	IN	denotes	after
T18237	10783-10795	JJ	denotes	intracardiac
T18239	10796-10799	NN	denotes	ink
T18238	10800-10810	NNS	denotes	injections
T18240	10811-10813	TO	denotes	to
T18241	10814-10823	VB	denotes	visualize
T18242	10824-10827	DT	denotes	the
T18244	10828-10838	VBG	denotes	developing
T18243	10839-10843	NNS	denotes	PAAs
T18245	10844-10846	IN	denotes	at
T18246	10847-10852	NN	denotes	E11.0
T18247	10853-10854	-LRB-	denotes	(
T18249	10854-10855	NN	denotes	A
T18250	10855-10856	,	denotes	,
T18248	10856-10857	NN	denotes	B
T18251	10857-10858	-RRB-	denotes	)
T18252	10858-10860	,	denotes	,
T18253	10860-10865	NN	denotes	E12.0
T18254	10866-10867	-LRB-	denotes	(
T18256	10867-10868	NN	denotes	C
T18257	10868-10870	,	denotes	,
T18255	10870-10871	NN	denotes	D
T18258	10871-10872	-RRB-	denotes	)
T18259	10873-10876	CC	denotes	and
T18260	10877-10882	NN	denotes	E13.0
T18261	10883-10884	-LRB-	denotes	(
T18263	10884-10885	NN	denotes	E
T18264	10885-10887	,	denotes	,
T18262	10887-10888	NN	denotes	F
T18265	10888-10889	-RRB-	denotes	)
T18266	10890-10892	IN	denotes	in
T18267	10893-10901	NNS	denotes	controls
T18268	10902-10903	-LRB-	denotes	(
T18270	10903-10904	NN	denotes	A
T18271	10904-10906	,	denotes	,
T18272	10906-10907	NN	denotes	C
T18273	10907-10909	,	denotes	,
T18269	10909-10910	NN	denotes	E
T18274	10910-10911	-RRB-	denotes	)
T18275	10912-10915	CC	denotes	and
T18276	10916-10920	NN	denotes	Alk5
T18278	10920-10921	HYPH	denotes	/
T18279	10921-10925	NN	denotes	Wnt1
T18280	10925-10926	HYPH	denotes	-
T18277	10926-10929	NN	denotes	Cre
T18281	10930-10937	NNS	denotes	mutants
T18282	10938-10939	-LRB-	denotes	(
T18284	10939-10940	NN	denotes	B
T18285	10940-10942	,	denotes	,
T18286	10942-10943	NN	denotes	D
T18287	10943-10945	,	denotes	,
T18283	10945-10946	NN	denotes	F
T18288	10946-10947	-RRB-	denotes	)
T18289	10947-10948	.	denotes	.
T18290	10948-10998	sentence	denotes	Arrow in A points to the regressing carotid duct.
T18291	10949-10954	NN	denotes	Arrow
T18293	10955-10957	IN	denotes	in
T18294	10958-10959	NN	denotes	A
T18292	10960-10966	VBZ	denotes	points
T18295	10967-10969	IN	denotes	to
T18296	10970-10973	DT	denotes	the
T18298	10974-10984	VBG	denotes	regressing
T18299	10985-10992	JJ	denotes	carotid
T18297	10993-10997	NN	denotes	duct
T18300	10997-10998	.	denotes	.
T18301	10998-11091	sentence	denotes	Asterisk in B depicts the corresponding structure in the mutant with no signs of regression.
T18302	10999-11007	NN	denotes	Asterisk
T18304	11008-11010	IN	denotes	in
T18305	11011-11012	NN	denotes	B
T18303	11013-11020	VBZ	denotes	depicts
T18306	11021-11024	DT	denotes	the
T18308	11025-11038	VBG	denotes	corresponding
T18307	11039-11048	NN	denotes	structure
T18309	11049-11051	IN	denotes	in
T18310	11052-11055	DT	denotes	the
T18311	11056-11062	NN	denotes	mutant
T18312	11063-11067	IN	denotes	with
T18313	11068-11070	DT	denotes	no
T18314	11071-11076	NNS	denotes	signs
T18315	11077-11079	IN	denotes	of
T18316	11080-11090	NN	denotes	regression
T18317	11090-11091	.	denotes	.
T18318	11091-11191	sentence	denotes	Asterisk in D illustrates the aberrant regression of the dorsal aorta between the 4th and 6th PAAs.
T18319	11092-11100	NN	denotes	Asterisk
T18321	11101-11103	IN	denotes	in
T18322	11104-11105	NN	denotes	D
T18320	11106-11117	VBZ	denotes	illustrates
T18323	11118-11121	DT	denotes	the
T18325	11122-11130	JJ	denotes	aberrant
T18324	11131-11141	NN	denotes	regression
T18326	11142-11144	IN	denotes	of
T18327	11145-11148	DT	denotes	the
T18329	11149-11155	JJ	denotes	dorsal
T18328	11156-11161	NN	denotes	aorta
T18330	11162-11169	IN	denotes	between
T18331	11170-11173	DT	denotes	the
T18333	11174-11177	JJ	denotes	4th
T18334	11178-11181	CC	denotes	and
T18335	11182-11185	JJ	denotes	6th
T18332	11186-11190	NNS	denotes	PAAs
T18336	11190-11191	.	denotes	.
T18337	11191-11247	sentence	denotes	PT, pulmonary trunk; Ao, Aorta; TA, truncus arteriosus.
T18338	11192-11194	NN	denotes	PT
T18339	11194-11196	,	denotes	,
T18340	11196-11205	JJ	denotes	pulmonary
T18341	11206-11211	NN	denotes	trunk
T18342	11211-11212	:	denotes	;
T18343	11213-11215	NN	denotes	Ao
T18344	11215-11217	,	denotes	,
T18345	11217-11222	NN	denotes	Aorta
T18346	11222-11223	:	denotes	;
T18347	11224-11226	NN	denotes	TA
T18348	11226-11228	,	denotes	,
T18349	11228-11235	NN	denotes	truncus
T18350	11236-11246	NN	denotes	arteriosus
T18351	11246-11247	.	denotes	.
T6182	11248-11254	IN	denotes	Around
T6184	11255-11260	NN	denotes	E11.5
T6185	11260-11262	,	denotes	,
T6186	11262-11265	DT	denotes	the
T6188	11266-11272	JJ	denotes	aortic
T6187	11273-11276	NN	denotes	sac
T6189	11277-11285	RB	denotes	normally
T6183	11286-11291	VBZ	denotes	forms
T6190	11292-11293	DT	denotes	a
T6192	11294-11305	JJ	denotes	distinctive
T6193	11306-11307	NN	denotes	T
T6195	11307-11308	HYPH	denotes	-
T6194	11308-11314	VBN	denotes	shaped
T6191	11315-11324	NN	denotes	structure
T6196	11324-11326	,	denotes	,
T6197	11326-11328	IN	denotes	as
T6198	11329-11333	VBN	denotes	seen
T6199	11334-11336	IN	denotes	in
T6200	11337-11344	JJ	denotes	frontal
T6201	11345-11353	NNS	denotes	sections
T6202	11354-11356	IN	denotes	of
T6203	11357-11360	DT	denotes	the
T6205	11361-11368	NN	denotes	control
T6204	11369-11375	NN	denotes	sample
T6206	11376-11378	IN	denotes	in
T6207	11379-11383	NN	denotes	Fig.
T6208	11384-11385	CD	denotes	3
T6209	11385-11386	-LRB-	denotes	(
T6211	11386-11387	NN	denotes	A
T6212	11387-11388	,	denotes	,
T6210	11388-11389	NN	denotes	C
T6213	11389-11390	-RRB-	denotes	)
T6214	11390-11391	.	denotes	.
T6215	11391-11592	sentence	denotes	Subsequently, the right horn of this structure transforms into the prospective brachiocephalic artery, while the left horn together with the left 4th PAA gives rise to the definitive aortic arch [23].
T6216	11392-11404	RB	denotes	Subsequently
T6218	11404-11406	,	denotes	,
T6219	11406-11409	DT	denotes	the
T6221	11410-11415	JJ	denotes	right
T6220	11416-11420	NN	denotes	horn
T6222	11421-11423	IN	denotes	of
T6223	11424-11428	DT	denotes	this
T6224	11429-11438	NN	denotes	structure
T6217	11439-11449	VBZ	denotes	transforms
T6225	11450-11454	IN	denotes	into
T6226	11455-11458	DT	denotes	the
T6228	11459-11470	JJ	denotes	prospective
T6229	11471-11486	JJ	denotes	brachiocephalic
T6227	11487-11493	NN	denotes	artery
T6230	11493-11495	,	denotes	,
T6231	11495-11500	IN	denotes	while
T6233	11501-11504	DT	denotes	the
T6235	11505-11509	JJ	denotes	left
T6234	11510-11514	NN	denotes	horn
T6236	11515-11523	RB	denotes	together
T6237	11524-11528	IN	denotes	with
T6238	11529-11532	DT	denotes	the
T6240	11533-11537	JJ	denotes	left
T6241	11538-11541	JJ	denotes	4th
T6239	11542-11545	NN	denotes	PAA
T6232	11546-11551	VBZ	denotes	gives
T6242	11552-11556	NN	denotes	rise
T6243	11557-11559	IN	denotes	to
T6244	11560-11563	DT	denotes	the
T6246	11564-11574	JJ	denotes	definitive
T6247	11575-11581	JJ	denotes	aortic
T6245	11582-11586	NN	denotes	arch
T6248	11587-11588	-LRB-	denotes	[
T6249	11588-11590	CD	denotes	23
T6250	11590-11591	-RRB-	denotes	]
T6251	11591-11592	.	denotes	.
T6252	11592-11670	sentence	denotes	In Alk5/Wnt1-Cre mutants, the T-shaped aortic sac failed to form (Fig. 3B,D).
T6253	11593-11595	IN	denotes	In
T6255	11596-11600	NN	denotes	Alk5
T6257	11600-11601	HYPH	denotes	/
T6258	11601-11605	NN	denotes	Wnt1
T6259	11605-11606	HYPH	denotes	-
T6256	11606-11609	NN	denotes	Cre
T6260	11610-11617	NNS	denotes	mutants
T6261	11617-11619	,	denotes	,
T6262	11619-11622	DT	denotes	the
T6264	11623-11624	NN	denotes	T
T6266	11624-11625	HYPH	denotes	-
T6265	11625-11631	VBN	denotes	shaped
T6267	11632-11638	JJ	denotes	aortic
T6263	11639-11642	NN	denotes	sac
T6254	11643-11649	VBD	denotes	failed
T6268	11650-11652	TO	denotes	to
T6269	11653-11657	VB	denotes	form
T6270	11658-11659	-LRB-	denotes	(
T6272	11659-11663	NN	denotes	Fig.
T6273	11664-11666	NN	denotes	3B
T6274	11666-11667	,	denotes	,
T6271	11667-11668	NN	denotes	D
T6275	11668-11669	-RRB-	denotes	)
T6276	11669-11670	.	denotes	.
T6277	11670-11825	sentence	denotes	Instead, the truncus bifurcated to a left and right arm, which further branched to the PAAs, particularly to the predominant pair of 6th PAAs (Fig. 3B,D).
T6278	11671-11678	RB	denotes	Instead
T6280	11678-11680	,	denotes	,
T6281	11680-11683	DT	denotes	the
T6282	11684-11691	NN	denotes	truncus
T6279	11692-11702	VBD	denotes	bifurcated
T6283	11703-11705	IN	denotes	to
T6284	11706-11707	DT	denotes	a
T6286	11708-11712	JJ	denotes	left
T6287	11713-11716	CC	denotes	and
T6288	11717-11722	JJ	denotes	right
T6285	11723-11726	NN	denotes	arm
T6289	11726-11728	,	denotes	,
T6290	11728-11733	WDT	denotes	which
T6292	11734-11741	RB	denotes	further
T6291	11742-11750	VBD	denotes	branched
T6293	11751-11753	IN	denotes	to
T6294	11754-11757	DT	denotes	the
T6295	11758-11762	NNS	denotes	PAAs
T6296	11762-11764	,	denotes	,
T6297	11764-11776	RB	denotes	particularly
T6298	11777-11779	IN	denotes	to
T6299	11780-11783	DT	denotes	the
T6301	11784-11795	JJ	denotes	predominant
T6300	11796-11800	NN	denotes	pair
T6302	11801-11803	IN	denotes	of
T6303	11804-11807	JJ	denotes	6th
T6304	11808-11812	NNS	denotes	PAAs
T6305	11813-11814	-LRB-	denotes	(
T6307	11814-11818	NN	denotes	Fig.
T6308	11819-11821	NN	denotes	3B
T6309	11821-11822	,	denotes	,
T6306	11822-11823	NN	denotes	D
T6310	11823-11824	-RRB-	denotes	)
T6311	11824-11825	.	denotes	.
T6312	11825-12085	sentence	denotes	The observed phenotype is consistent with the absence or severe hypoplasia of structures derived from the aortic sac in late stage embryos (E17), e.g., the missing brachiocephalic artery and severe shortening of the ascending truncus as shown in the Figure 1.
T6313	11826-11829	DT	denotes	The
T6315	11830-11838	VBN	denotes	observed
T6314	11839-11848	NN	denotes	phenotype
T6316	11849-11851	VBZ	denotes	is
T6317	11852-11862	JJ	denotes	consistent
T6318	11863-11867	IN	denotes	with
T6319	11868-11871	DT	denotes	the
T6320	11872-11879	NN	denotes	absence
T6321	11880-11882	CC	denotes	or
T6322	11883-11889	JJ	denotes	severe
T6323	11890-11900	NN	denotes	hypoplasia
T6324	11901-11903	IN	denotes	of
T6325	11904-11914	NNS	denotes	structures
T6326	11915-11922	VBN	denotes	derived
T6327	11923-11927	IN	denotes	from
T6328	11928-11931	DT	denotes	the
T6330	11932-11938	JJ	denotes	aortic
T6329	11939-11942	NN	denotes	sac
T6331	11943-11945	IN	denotes	in
T6332	11946-11950	JJ	denotes	late
T6334	11951-11956	NN	denotes	stage
T6333	11957-11964	NNS	denotes	embryos
T6335	11965-11966	-LRB-	denotes	(
T6336	11966-11969	NN	denotes	E17
T6337	11969-11970	-RRB-	denotes	)
T6338	11970-11972	,	denotes	,
T6339	11972-11976	FW	denotes	e.g.
T6341	11976-11978	,	denotes	,
T6342	11978-11981	DT	denotes	the
T6343	11982-11989	VBG	denotes	missing
T6344	11990-12005	JJ	denotes	brachiocephalic
T6340	12006-12012	NN	denotes	artery
T6345	12013-12016	CC	denotes	and
T6346	12017-12023	JJ	denotes	severe
T6347	12024-12034	NN	denotes	shortening
T6348	12035-12037	IN	denotes	of
T6349	12038-12041	DT	denotes	the
T6351	12042-12051	VBG	denotes	ascending
T6350	12052-12059	NN	denotes	truncus
T6352	12060-12062	IN	denotes	as
T6353	12063-12068	VBN	denotes	shown
T6354	12069-12071	IN	denotes	in
T6355	12072-12075	DT	denotes	the
T6356	12076-12082	NN	denotes	Figure
T6357	12083-12084	CD	denotes	1
T6358	12084-12085	.	denotes	.
T18564	12096-12104	JJ	denotes	Abnormal
T18566	12105-12111	JJ	denotes	Aortic
T18565	12112-12115	NN	denotes	Sac
T18567	12116-12118	IN	denotes	in
T18568	12119-12123	NN	denotes	Alk5
T18570	12123-12124	HYPH	denotes	/
T18571	12124-12128	NN	denotes	Wnt1
T18572	12128-12129	HYPH	denotes	-
T18569	12129-12132	NN	denotes	Cre
T18573	12133-12140	NNS	denotes	mutants
T18574	12140-12141	.	denotes	.
T18575	12141-12259	sentence	denotes	Alk5/Wnt1-Cre mutants (B, D) fail to form the typical T-shaped structure of the aortic sac seen in controls at E11.5.
T18576	12142-12146	NN	denotes	Alk5
T18578	12146-12147	HYPH	denotes	/
T18579	12147-12151	NN	denotes	Wnt1
T18580	12151-12152	HYPH	denotes	-
T18577	12152-12155	NN	denotes	Cre
T18581	12156-12163	NNS	denotes	mutants
T18583	12164-12165	-LRB-	denotes	(
T18585	12165-12166	NN	denotes	B
T18586	12166-12168	,	denotes	,
T18584	12168-12169	NN	denotes	D
T18587	12169-12170	-RRB-	denotes	)
T18582	12171-12175	VBP	denotes	fail
T18588	12176-12178	TO	denotes	to
T18589	12179-12183	VB	denotes	form
T18590	12184-12187	DT	denotes	the
T18592	12188-12195	JJ	denotes	typical
T18593	12196-12197	NN	denotes	T
T18595	12197-12198	HYPH	denotes	-
T18594	12198-12204	VBN	denotes	shaped
T18591	12205-12214	NN	denotes	structure
T18596	12215-12217	IN	denotes	of
T18597	12218-12221	DT	denotes	the
T18599	12222-12228	JJ	denotes	aortic
T18598	12229-12232	NN	denotes	sac
T18600	12233-12237	VBN	denotes	seen
T18601	12238-12240	IN	denotes	in
T18602	12241-12249	NNS	denotes	controls
T18603	12250-12252	IN	denotes	at
T18604	12253-12258	NN	denotes	E11.5
T18605	12258-12259	.	denotes	.
T18606	12259-12267	sentence	denotes	(A, C).
T18607	12260-12261	-LRB-	denotes	(
T18608	12261-12262	LS	denotes	A
T18609	12262-12264	,	denotes	,
T18610	12264-12265	LS	denotes	C
T18611	12265-12266	-RRB-	denotes	)
T18612	12266-12267	.	denotes	.
T18613	12267-12349	sentence	denotes	A-B, frontal image of ink-injected embryos; C-D, frontal sections (H&E staining).
T18614	12268-12269	LS	denotes	A
T18616	12269-12270	SYM	denotes	-
T18617	12270-12271	LS	denotes	B
T18618	12271-12273	,	denotes	,
T18619	12273-12280	JJ	denotes	frontal
T18615	12281-12286	NN	denotes	image
T18620	12287-12289	IN	denotes	of
T18621	12290-12293	NN	denotes	ink
T18623	12293-12294	HYPH	denotes	-
T18622	12294-12302	VBN	denotes	injected
T18624	12303-12310	NNS	denotes	embryos
T18625	12310-12311	:	denotes	;
T18626	12312-12313	LS	denotes	C
T18628	12313-12314	SYM	denotes	-
T18629	12314-12315	LS	denotes	D
T18630	12315-12317	,	denotes	,
T18631	12317-12324	JJ	denotes	frontal
T18627	12325-12333	NNS	denotes	sections
T18632	12334-12335	-LRB-	denotes	(
T18634	12335-12336	NN	denotes	H
T18635	12336-12337	CC	denotes	&
T18636	12337-12338	NN	denotes	E
T18633	12339-12347	NN	denotes	staining
T18637	12347-12348	-RRB-	denotes	)
T18638	12348-12349	.	denotes	.
T18639	12349-12503	sentence	denotes	Arrows in A and B point to the level of section shown in C and D (red arrows in C and D point to the aortic sac of the control and mutant, respectively).
T18640	12350-12356	NNS	denotes	Arrows
T18642	12357-12359	IN	denotes	in
T18643	12360-12361	NN	denotes	A
T18644	12362-12365	CC	denotes	and
T18645	12366-12367	NN	denotes	B
T18641	12368-12373	VBP	denotes	point
T18647	12374-12376	IN	denotes	to
T18648	12377-12380	DT	denotes	the
T18649	12381-12386	NN	denotes	level
T18650	12387-12389	IN	denotes	of
T18651	12390-12397	NN	denotes	section
T18652	12398-12403	VBN	denotes	shown
T18653	12404-12406	IN	denotes	in
T18654	12407-12408	NN	denotes	C
T18655	12409-12412	CC	denotes	and
T18656	12413-12414	NN	denotes	D
T18657	12415-12416	-LRB-	denotes	(
T18658	12416-12419	JJ	denotes	red
T18659	12420-12426	NNS	denotes	arrows
T18660	12427-12429	IN	denotes	in
T18661	12430-12431	NN	denotes	C
T18662	12432-12435	CC	denotes	and
T18663	12436-12437	NN	denotes	D
T18646	12438-12443	VBP	denotes	point
T18664	12444-12446	IN	denotes	to
T18665	12447-12450	DT	denotes	the
T18667	12451-12457	JJ	denotes	aortic
T18666	12458-12461	NN	denotes	sac
T18668	12462-12464	IN	denotes	of
T18669	12465-12468	DT	denotes	the
T18670	12469-12476	NN	denotes	control
T18671	12477-12480	CC	denotes	and
T18672	12481-12487	NN	denotes	mutant
T18673	12487-12489	,	denotes	,
T18674	12489-12501	RB	denotes	respectively
T18675	12501-12502	-RRB-	denotes	)
T18676	12502-12503	.	denotes	.
T6831	12505-12512	JJ	denotes	Cardiac
T6832	12513-12517	NNS	denotes	NCCs
T6834	12518-12527	JJ	denotes	deficient
T6835	12528-12530	IN	denotes	in
T6836	12531-12535	NN	denotes	Alk5
T6837	12536-12539	MD	denotes	can
T6833	12540-12548	VB	denotes	populate
T6838	12549-12552	DT	denotes	the
T6840	12553-12560	NN	denotes	outflow
T6839	12561-12566	NN	denotes	tract
T6841	12566-12692	sentence	denotes	Next we used the R26R lineage-tracing assay to determine whether CNCCs could appropriately populate the outflow tract region.
T6842	12567-12571	RB	denotes	Next
T6844	12572-12574	PRP	denotes	we
T6843	12575-12579	VBD	denotes	used
T6845	12580-12583	DT	denotes	the
T6847	12584-12588	NN	denotes	R26R
T6848	12589-12596	NN	denotes	lineage
T6849	12596-12597	HYPH	denotes	-
T6850	12597-12604	VBG	denotes	tracing
T6846	12605-12610	NN	denotes	assay
T6851	12611-12613	TO	denotes	to
T6852	12614-12623	VB	denotes	determine
T6853	12624-12631	IN	denotes	whether
T6855	12632-12637	NNS	denotes	CNCCs
T6856	12638-12643	MD	denotes	could
T6857	12644-12657	RB	denotes	appropriately
T6854	12658-12666	VB	denotes	populate
T6858	12667-12670	DT	denotes	the
T6860	12671-12678	NN	denotes	outflow
T6861	12679-12684	NN	denotes	tract
T6859	12685-12691	NN	denotes	region
T6862	12691-12692	.	denotes	.
T6863	12692-12861	sentence	denotes	Briefly, Alk5Flox/Flox mice were crossed with the ROSA26 Cre reporter mice, and subsequently Alk5Flox/Flox;R26R(+/+) females were crossed with Alk5KO/WT;Wnt1-Cre males.
T6864	12693-12700	RB	denotes	Briefly
T6866	12700-12702	,	denotes	,
T6867	12702-12710	NN	denotes	Alk5Flox
T6869	12710-12711	HYPH	denotes	/
T6868	12711-12715	NN	denotes	Flox
T6870	12716-12720	NNS	denotes	mice
T6871	12721-12725	VBD	denotes	were
T6865	12726-12733	VBN	denotes	crossed
T6872	12734-12738	IN	denotes	with
T6873	12739-12742	DT	denotes	the
T6875	12743-12749	NN	denotes	ROSA26
T6877	12750-12753	NN	denotes	Cre
T6876	12754-12762	NN	denotes	reporter
T6874	12763-12767	NNS	denotes	mice
T6878	12767-12769	,	denotes	,
T6879	12769-12772	CC	denotes	and
T6880	12773-12785	RB	denotes	subsequently
T6882	12786-12794	NN	denotes	Alk5Flox
T6884	12794-12795	HYPH	denotes	/
T6883	12795-12799	NN	denotes	Flox
T6886	12799-12800	:	denotes	;
T6887	12800-12804	NN	denotes	R26R
T6888	12804-12805	-LRB-	denotes	(
T6889	12805-12806	SYM	denotes	+
T6890	12806-12807	HYPH	denotes	/
T6885	12807-12808	SYM	denotes	+
T6892	12808-12809	-RRB-	denotes	)
T6891	12810-12817	NNS	denotes	females
T6893	12818-12822	VBD	denotes	were
T6881	12823-12830	VBN	denotes	crossed
T6894	12831-12835	IN	denotes	with
T6895	12836-12842	NN	denotes	Alk5KO
T6897	12842-12843	HYPH	denotes	/
T6898	12843-12845	NN	denotes	WT
T6899	12845-12846	:	denotes	;
T6900	12846-12850	NN	denotes	Wnt1
T6901	12850-12851	HYPH	denotes	-
T6896	12851-12854	NN	denotes	Cre
T6902	12855-12860	NNS	denotes	males
T6903	12860-12861	.	denotes	.
T6904	12861-13035	sentence	denotes	The resulting embryos had the NC-lineage permanently labeled with β-galactosidase expression, and displayed identical phenotypes to those obtained without the R26R reporter.
T6905	12862-12865	DT	denotes	The
T6907	12866-12875	VBG	denotes	resulting
T6906	12876-12883	NNS	denotes	embryos
T6908	12884-12887	VBD	denotes	had
T6909	12888-12891	DT	denotes	the
T6911	12892-12894	NN	denotes	NC
T6912	12894-12895	HYPH	denotes	-
T6910	12895-12902	NN	denotes	lineage
T6914	12903-12914	RB	denotes	permanently
T6913	12915-12922	VBN	denotes	labeled
T6915	12923-12927	IN	denotes	with
T6916	12928-12929	NN	denotes	β
T6918	12929-12930	HYPH	denotes	-
T6919	12930-12943	NN	denotes	galactosidase
T6917	12944-12954	NN	denotes	expression
T6920	12954-12956	,	denotes	,
T6921	12956-12959	CC	denotes	and
T6922	12960-12969	VBD	denotes	displayed
T6923	12970-12979	JJ	denotes	identical
T6924	12980-12990	NNS	denotes	phenotypes
T6925	12991-12993	IN	denotes	to
T6926	12994-12999	DT	denotes	those
T6927	13000-13008	VBN	denotes	obtained
T6928	13009-13016	IN	denotes	without
T6929	13017-13020	DT	denotes	the
T6931	13021-13025	NN	denotes	R26R
T6930	13026-13034	NN	denotes	reporter
T6932	13034-13035	.	denotes	.
T6933	13035-13187	sentence	denotes	Staining of embryos for β-galactosidase at E8-E11 did not reveal detectable differences in NCC migration between mutants and controls (data not shown).
T6934	13036-13044	NN	denotes	Staining
T6936	13045-13047	IN	denotes	of
T6937	13048-13055	NNS	denotes	embryos
T6938	13056-13059	IN	denotes	for
T6939	13060-13061	NN	denotes	β
T6941	13061-13062	HYPH	denotes	-
T6940	13062-13075	NN	denotes	galactosidase
T6942	13076-13078	IN	denotes	at
T6943	13079-13081	NN	denotes	E8
T6944	13081-13082	SYM	denotes	-
T6945	13082-13085	NN	denotes	E11
T6946	13086-13089	VBD	denotes	did
T6947	13090-13093	RB	denotes	not
T6935	13094-13100	VB	denotes	reveal
T6948	13101-13111	JJ	denotes	detectable
T6949	13112-13123	NNS	denotes	differences
T6950	13124-13126	IN	denotes	in
T6951	13127-13130	NN	denotes	NCC
T6952	13131-13140	NN	denotes	migration
T6953	13141-13148	IN	denotes	between
T6954	13149-13156	NNS	denotes	mutants
T6955	13157-13160	CC	denotes	and
T6956	13161-13169	NNS	denotes	controls
T6957	13170-13171	-LRB-	denotes	(
T6959	13171-13175	NNS	denotes	data
T6960	13176-13179	RB	denotes	not
T6958	13180-13185	VBN	denotes	shown
T6961	13185-13186	-RRB-	denotes	)
T6962	13186-13187	.	denotes	.
T6963	13187-13494	sentence	denotes	Similarly, serial transverse sectioning of whole mount embryos (E10-E12) and subsequent analysis of positively stained cells in the OFT region demonstrated that CNCCs deficient in Alk5 were capable of populating the PAAs, aortic sac and conotruncal ridges at a level comparable to that of controls (Fig 4).
T6964	13188-13197	RB	denotes	Similarly
T6966	13197-13199	,	denotes	,
T6967	13199-13205	JJ	denotes	serial
T6969	13206-13216	JJ	denotes	transverse
T6968	13217-13227	NN	denotes	sectioning
T6970	13228-13230	IN	denotes	of
T6971	13231-13236	JJ	denotes	whole
T6972	13237-13242	NN	denotes	mount
T6973	13243-13250	NNS	denotes	embryos
T6974	13251-13252	-LRB-	denotes	(
T6975	13252-13255	NN	denotes	E10
T6976	13255-13256	SYM	denotes	-
T6977	13256-13259	NN	denotes	E12
T6978	13259-13260	-RRB-	denotes	)
T6979	13261-13264	CC	denotes	and
T6980	13265-13275	JJ	denotes	subsequent
T6981	13276-13284	NN	denotes	analysis
T6982	13285-13287	IN	denotes	of
T6983	13288-13298	RB	denotes	positively
T6984	13299-13306	VBN	denotes	stained
T6985	13307-13312	NNS	denotes	cells
T6986	13313-13315	IN	denotes	in
T6987	13316-13319	DT	denotes	the
T6989	13320-13323	NN	denotes	OFT
T6988	13324-13330	NN	denotes	region
T6965	13331-13343	VBD	denotes	demonstrated
T6990	13344-13348	IN	denotes	that
T6992	13349-13354	NNS	denotes	CNCCs
T6993	13355-13364	JJ	denotes	deficient
T6994	13365-13367	IN	denotes	in
T6995	13368-13372	NN	denotes	Alk5
T6991	13373-13377	VBD	denotes	were
T6996	13378-13385	JJ	denotes	capable
T6997	13386-13388	IN	denotes	of
T6998	13389-13399	VBG	denotes	populating
T6999	13400-13403	DT	denotes	the
T7000	13404-13408	NNS	denotes	PAAs
T7001	13408-13410	,	denotes	,
T7002	13410-13416	JJ	denotes	aortic
T7003	13417-13420	NN	denotes	sac
T7004	13421-13424	CC	denotes	and
T7005	13425-13436	JJ	denotes	conotruncal
T7006	13437-13443	NNS	denotes	ridges
T7007	13444-13446	IN	denotes	at
T7008	13447-13448	DT	denotes	a
T7009	13449-13454	NN	denotes	level
T7010	13455-13465	JJ	denotes	comparable
T7011	13466-13468	IN	denotes	to
T7012	13469-13473	DT	denotes	that
T7013	13474-13476	IN	denotes	of
T7014	13477-13485	NNS	denotes	controls
T7015	13486-13487	-LRB-	denotes	(
T7016	13487-13490	NN	denotes	Fig
T7017	13491-13492	CD	denotes	4
T7018	13492-13493	-RRB-	denotes	)
T7019	13493-13494	.	denotes	.
T7020	13494-13656	sentence	denotes	To conclude, the observed phenotypes in Alk5/Wnt1-Cre mutants were certainly not due to defective migration of CNCCs to the pharyngeal and outflow tract regions.
T7021	13495-13497	TO	denotes	To
T7022	13498-13506	VB	denotes	conclude
T7024	13506-13508	,	denotes	,
T7025	13508-13511	DT	denotes	the
T7027	13512-13520	VBN	denotes	observed
T7026	13521-13531	NNS	denotes	phenotypes
T7028	13532-13534	IN	denotes	in
T7029	13535-13539	NN	denotes	Alk5
T7031	13539-13540	HYPH	denotes	/
T7032	13540-13544	NN	denotes	Wnt1
T7033	13544-13545	HYPH	denotes	-
T7030	13545-13548	NN	denotes	Cre
T7034	13549-13556	NNS	denotes	mutants
T7023	13557-13561	VBD	denotes	were
T7035	13562-13571	RB	denotes	certainly
T7036	13572-13575	RB	denotes	not
T7037	13576-13579	IN	denotes	due
T7038	13580-13582	IN	denotes	to
T7039	13583-13592	JJ	denotes	defective
T7040	13593-13602	NN	denotes	migration
T7041	13603-13605	IN	denotes	of
T7042	13606-13611	NNS	denotes	CNCCs
T7043	13612-13614	IN	denotes	to
T7044	13615-13618	DT	denotes	the
T7046	13619-13629	JJ	denotes	pharyngeal
T7047	13630-13633	CC	denotes	and
T7048	13634-13641	NN	denotes	outflow
T7049	13642-13647	NN	denotes	tract
T7045	13648-13655	NNS	denotes	regions
T7050	13655-13656	.	denotes	.
T18898	13667-13673	JJ	denotes	Normal
T18900	13674-13681	JJ	denotes	cardiac
T18901	13682-13685	NN	denotes	NCC
T18899	13686-13695	NN	denotes	migration
T18902	13696-13698	IN	denotes	in
T18903	13699-13703	NN	denotes	Alk5
T18905	13703-13704	HYPH	denotes	/
T18906	13704-13708	NN	denotes	Wnt1
T18907	13708-13709	HYPH	denotes	-
T18904	13709-13712	NN	denotes	Cre
T18908	13713-13720	NNS	denotes	mutants
T18909	13720-13721	.	denotes	.
T18910	13721-13882	sentence	denotes	The OFT of controls (A, C, E) and Alk5/Wnt1-Cre mutants (B, D, F) display similar staining patterns when analyzed using the R26R lineage tracing assay at E11.0.
T18911	13722-13725	DT	denotes	The
T18912	13726-13729	NN	denotes	OFT
T18914	13730-13732	IN	denotes	of
T18915	13733-13741	NNS	denotes	controls
T18916	13742-13743	-LRB-	denotes	(
T18918	13743-13744	NN	denotes	A
T18919	13744-13746	,	denotes	,
T18920	13746-13747	NN	denotes	C
T18921	13747-13749	,	denotes	,
T18917	13749-13750	NN	denotes	E
T18922	13750-13751	-RRB-	denotes	)
T18923	13752-13755	CC	denotes	and
T18924	13756-13760	NN	denotes	Alk5
T18926	13760-13761	HYPH	denotes	/
T18927	13761-13765	NN	denotes	Wnt1
T18928	13765-13766	HYPH	denotes	-
T18925	13766-13769	NN	denotes	Cre
T18929	13770-13777	NNS	denotes	mutants
T18930	13778-13779	-LRB-	denotes	(
T18932	13779-13780	NN	denotes	B
T18933	13780-13782	,	denotes	,
T18934	13782-13783	NN	denotes	D
T18935	13783-13785	,	denotes	,
T18931	13785-13786	NN	denotes	F
T18936	13786-13787	-RRB-	denotes	)
T18913	13788-13795	VBP	denotes	display
T18937	13796-13803	JJ	denotes	similar
T18939	13804-13812	NN	denotes	staining
T18938	13813-13821	NNS	denotes	patterns
T18940	13822-13826	WRB	denotes	when
T18941	13827-13835	VBN	denotes	analyzed
T18942	13836-13841	VBG	denotes	using
T18943	13842-13845	DT	denotes	the
T18945	13846-13850	NN	denotes	R26R
T18946	13851-13858	NN	denotes	lineage
T18947	13859-13866	NN	denotes	tracing
T18944	13867-13872	NN	denotes	assay
T18948	13873-13875	IN	denotes	at
T18949	13876-13881	NN	denotes	E11.0
T18950	13881-13882	.	denotes	.
T18951	13882-14007	sentence	denotes	A-B, whole mount staining (left lateral image); C-F, transverse sections on the level of the 4th (C, D) and 6th (E, F) PAAs.
T18952	13883-13884	LS	denotes	A
T18954	13884-13885	SYM	denotes	-
T18955	13885-13886	LS	denotes	B
T18956	13886-13888	,	denotes	,
T18957	13888-13893	JJ	denotes	whole
T18958	13894-13899	NN	denotes	mount
T18953	13900-13908	NN	denotes	staining
T18959	13909-13910	-LRB-	denotes	(
T18961	13910-13914	JJ	denotes	left
T18962	13915-13922	JJ	denotes	lateral
T18960	13923-13928	NN	denotes	image
T18963	13928-13929	-RRB-	denotes	)
T18964	13929-13930	:	denotes	;
T18965	13931-13932	LS	denotes	C
T18967	13932-13933	SYM	denotes	-
T18968	13933-13934	LS	denotes	F
T18969	13934-13936	,	denotes	,
T18970	13936-13946	JJ	denotes	transverse
T18966	13947-13955	NNS	denotes	sections
T18971	13956-13958	IN	denotes	on
T18972	13959-13962	DT	denotes	the
T18973	13963-13968	NN	denotes	level
T18974	13969-13971	IN	denotes	of
T18975	13972-13975	DT	denotes	the
T18977	13976-13979	JJ	denotes	4th
T18978	13980-13981	-LRB-	denotes	(
T18980	13981-13982	NN	denotes	C
T18981	13982-13984	,	denotes	,
T18979	13984-13985	NN	denotes	D
T18982	13985-13986	-RRB-	denotes	)
T18983	13987-13990	CC	denotes	and
T18984	13991-13994	JJ	denotes	6th
T18985	13995-13996	-LRB-	denotes	(
T18987	13996-13997	NN	denotes	E
T18988	13997-13999	,	denotes	,
T18986	13999-14000	NN	denotes	F
T18989	14000-14001	-RRB-	denotes	)
T18976	14002-14006	NNS	denotes	PAAs
T18990	14006-14007	.	denotes	.
T18991	14007-14108	sentence	denotes	Arrows (A-F) point to the most proximal location staining positive for the β-galactosidase activity.
T18992	14008-14014	NNS	denotes	Arrows
T18994	14015-14016	-LRB-	denotes	(
T18995	14016-14017	NN	denotes	A
T18996	14017-14018	SYM	denotes	-
T18997	14018-14019	NN	denotes	F
T18998	14019-14020	-RRB-	denotes	)
T18993	14021-14026	VBP	denotes	point
T18999	14027-14029	IN	denotes	to
T19000	14030-14033	DT	denotes	the
T19002	14034-14038	RBS	denotes	most
T19003	14039-14047	JJ	denotes	proximal
T19001	14048-14056	NN	denotes	location
T19004	14057-14065	VBG	denotes	staining
T19005	14066-14074	JJ	denotes	positive
T19006	14075-14078	IN	denotes	for
T19007	14079-14082	DT	denotes	the
T19009	14083-14084	NN	denotes	β
T19011	14084-14085	HYPH	denotes	-
T19010	14085-14098	NN	denotes	galactosidase
T19008	14099-14107	NN	denotes	activity
T19012	14107-14108	.	denotes	.
T8381	14110-14116	JJ	denotes	Aortic
T8382	14117-14120	NN	denotes	sac
T8384	14121-14124	CC	denotes	and
T8385	14125-14132	JJ	denotes	aortico
T8387	14132-14133	HYPH	denotes	-
T8386	14133-14142	JJ	denotes	pulmonary
T8388	14143-14149	JJ	denotes	septal
T8383	14150-14157	NNS	denotes	defects
T8389	14158-14160	IN	denotes	in
T8390	14161-14165	NN	denotes	Alk5
T8392	14165-14166	HYPH	denotes	/
T8391	14166-14173	NN	denotes	Wnt1Cre
T8394	14174-14180	NN	denotes	mutant
T8393	14181-14188	NNS	denotes	embryos
T8395	14188-14340	sentence	denotes	Septation of the outflow tract lumen begins in a cranial-to-caudal direction, starting distally in the aortic sac and proceeding toward the heart [24].
T8396	14189-14198	NN	denotes	Septation
T8398	14199-14201	IN	denotes	of
T8399	14202-14205	DT	denotes	the
T8401	14206-14213	NN	denotes	outflow
T8402	14214-14219	NN	denotes	tract
T8400	14220-14225	NN	denotes	lumen
T8397	14226-14232	VBZ	denotes	begins
T8403	14233-14235	IN	denotes	in
T8404	14236-14237	DT	denotes	a
T8406	14238-14245	JJ	denotes	cranial
T8407	14245-14246	HYPH	denotes	-
T8408	14246-14248	IN	denotes	to
T8409	14248-14249	HYPH	denotes	-
T8410	14249-14255	JJ	denotes	caudal
T8405	14256-14265	NN	denotes	direction
T8411	14265-14267	,	denotes	,
T8412	14267-14275	VBG	denotes	starting
T8413	14276-14284	RB	denotes	distally
T8414	14285-14287	IN	denotes	in
T8415	14288-14291	DT	denotes	the
T8417	14292-14298	JJ	denotes	aortic
T8416	14299-14302	NN	denotes	sac
T8418	14303-14306	CC	denotes	and
T8419	14307-14317	VBG	denotes	proceeding
T8420	14318-14324	IN	denotes	toward
T8421	14325-14328	DT	denotes	the
T8422	14329-14334	NN	denotes	heart
T8423	14335-14336	-LRB-	denotes	[
T8424	14336-14338	CD	denotes	24
T8425	14338-14339	-RRB-	denotes	]
T8426	14339-14340	.	denotes	.
T8427	14340-14473	sentence	denotes	Initially, the condensed mesenchyme derived from the NC forms in the base of the aortic sac between the origins of 4th and 6th PAAs.
T8428	14341-14350	RB	denotes	Initially
T8430	14350-14352	,	denotes	,
T8431	14352-14355	DT	denotes	the
T8433	14356-14365	VBN	denotes	condensed
T8432	14366-14376	NN	denotes	mesenchyme
T8434	14377-14384	VBN	denotes	derived
T8435	14385-14389	IN	denotes	from
T8436	14390-14393	DT	denotes	the
T8437	14394-14396	NN	denotes	NC
T8429	14397-14402	VBZ	denotes	forms
T8438	14403-14405	IN	denotes	in
T8439	14406-14409	DT	denotes	the
T8440	14410-14414	NN	denotes	base
T8441	14415-14417	IN	denotes	of
T8442	14418-14421	DT	denotes	the
T8444	14422-14428	JJ	denotes	aortic
T8443	14429-14432	NN	denotes	sac
T8445	14433-14440	IN	denotes	between
T8446	14441-14444	DT	denotes	the
T8447	14445-14452	NNS	denotes	origins
T8448	14453-14455	IN	denotes	of
T8449	14456-14459	JJ	denotes	4th
T8451	14460-14463	CC	denotes	and
T8452	14464-14467	JJ	denotes	6th
T8450	14468-14472	NNS	denotes	PAAs
T8453	14472-14473	.	denotes	.
T8454	14473-14657	sentence	denotes	Subsequently, two prongs of the developing aortico-pulmonary (AP) septum extend into the truncal cushions and the aortico-pulmonary septation complex crosses the aortic sac cranially.
T8455	14474-14486	RB	denotes	Subsequently
T8457	14486-14488	,	denotes	,
T8458	14488-14491	CD	denotes	two
T8459	14492-14498	NNS	denotes	prongs
T8460	14499-14501	IN	denotes	of
T8461	14502-14505	DT	denotes	the
T8463	14506-14516	VBG	denotes	developing
T8464	14517-14524	JJ	denotes	aortico
T8466	14524-14525	HYPH	denotes	-
T8465	14525-14534	JJ	denotes	pulmonary
T8467	14535-14536	-LRB-	denotes	(
T8468	14536-14538	NN	denotes	AP
T8469	14538-14539	-RRB-	denotes	)
T8462	14540-14546	NN	denotes	septum
T8456	14547-14553	VBP	denotes	extend
T8470	14554-14558	IN	denotes	into
T8471	14559-14562	DT	denotes	the
T8473	14563-14570	JJ	denotes	truncal
T8472	14571-14579	NNS	denotes	cushions
T8474	14580-14583	CC	denotes	and
T8475	14584-14587	DT	denotes	the
T8477	14588-14595	JJ	denotes	aortico
T8479	14595-14596	HYPH	denotes	-
T8478	14596-14605	JJ	denotes	pulmonary
T8480	14606-14615	NN	denotes	septation
T8476	14616-14623	NN	denotes	complex
T8481	14624-14631	VBZ	denotes	crosses
T8482	14632-14635	DT	denotes	the
T8484	14636-14642	JJ	denotes	aortic
T8483	14643-14646	NN	denotes	sac
T8485	14647-14656	RB	denotes	cranially
T8486	14656-14657	.	denotes	.
T8487	14657-14897	sentence	denotes	In ink-injected control embryos at E11.5, a characteristic conotruncal transition separating the truncus and conus could be seen as a twisted configuration, resulting from a change in orientation of the truncal and conal cushions (Fig. 5).
T8488	14658-14660	IN	denotes	In
T8490	14661-14664	NN	denotes	ink
T8492	14664-14665	HYPH	denotes	-
T8491	14665-14673	VBN	denotes	injected
T8494	14674-14681	NN	denotes	control
T8493	14682-14689	NNS	denotes	embryos
T8495	14690-14692	IN	denotes	at
T8496	14693-14698	NN	denotes	E11.5
T8497	14698-14700	,	denotes	,
T8498	14700-14701	DT	denotes	a
T8500	14702-14716	JJ	denotes	characteristic
T8501	14717-14728	JJ	denotes	conotruncal
T8499	14729-14739	NN	denotes	transition
T8502	14740-14750	VBG	denotes	separating
T8503	14751-14754	DT	denotes	the
T8504	14755-14762	NN	denotes	truncus
T8505	14763-14766	CC	denotes	and
T8506	14767-14772	NN	denotes	conus
T8507	14773-14778	MD	denotes	could
T8508	14779-14781	VB	denotes	be
T8489	14782-14786	VBN	denotes	seen
T8509	14787-14789	IN	denotes	as
T8510	14790-14791	DT	denotes	a
T8512	14792-14799	VBN	denotes	twisted
T8511	14800-14813	NN	denotes	configuration
T8513	14813-14815	,	denotes	,
T8514	14815-14824	VBG	denotes	resulting
T8515	14825-14829	IN	denotes	from
T8516	14830-14831	DT	denotes	a
T8517	14832-14838	NN	denotes	change
T8518	14839-14841	IN	denotes	in
T8519	14842-14853	NN	denotes	orientation
T8520	14854-14856	IN	denotes	of
T8521	14857-14860	DT	denotes	the
T8523	14861-14868	JJ	denotes	truncal
T8524	14869-14872	CC	denotes	and
T8525	14873-14878	JJ	denotes	conal
T8522	14879-14887	NNS	denotes	cushions
T8526	14888-14889	-LRB-	denotes	(
T8527	14889-14893	NN	denotes	Fig.
T8528	14894-14895	CD	denotes	5
T8529	14895-14896	-RRB-	denotes	)
T8530	14896-14897	.	denotes	.
T8531	14897-15054	sentence	denotes	In contrast, in Alk5/Wnt1-Cre mutants the outflow tract appeared unusually straight, failing to demonstrate the distinct conotruncal transition (Fig. 5B,D).
T8532	14898-14900	IN	denotes	In
T8534	14901-14909	NN	denotes	contrast
T8535	14909-14911	,	denotes	,
T8536	14911-14913	IN	denotes	in
T8537	14914-14918	NN	denotes	Alk5
T8539	14918-14919	HYPH	denotes	/
T8540	14919-14923	NN	denotes	Wnt1
T8541	14923-14924	HYPH	denotes	-
T8538	14924-14927	NN	denotes	Cre
T8542	14928-14935	NNS	denotes	mutants
T8543	14936-14939	DT	denotes	the
T8545	14940-14947	NN	denotes	outflow
T8544	14948-14953	NN	denotes	tract
T8533	14954-14962	VBD	denotes	appeared
T8546	14963-14972	RB	denotes	unusually
T8547	14973-14981	JJ	denotes	straight
T8548	14981-14983	,	denotes	,
T8549	14983-14990	VBG	denotes	failing
T8550	14991-14993	TO	denotes	to
T8551	14994-15005	VB	denotes	demonstrate
T8552	15006-15009	DT	denotes	the
T8554	15010-15018	JJ	denotes	distinct
T8555	15019-15030	JJ	denotes	conotruncal
T8553	15031-15041	NN	denotes	transition
T8556	15042-15043	-LRB-	denotes	(
T8558	15043-15047	NN	denotes	Fig.
T8559	15048-15050	NN	denotes	5B
T8560	15050-15051	,	denotes	,
T8557	15051-15052	NN	denotes	D
T8561	15052-15053	-RRB-	denotes	)
T8562	15053-15054	.	denotes	.
T8563	15054-15137	sentence	denotes	This assay also clearly showed a dramatic reduction in the size of the aortic sac.
T8564	15055-15059	DT	denotes	This
T8565	15060-15065	NN	denotes	assay
T8567	15066-15070	RB	denotes	also
T8568	15071-15078	RB	denotes	clearly
T8566	15079-15085	VBD	denotes	showed
T8569	15086-15087	DT	denotes	a
T8571	15088-15096	JJ	denotes	dramatic
T8570	15097-15106	NN	denotes	reduction
T8572	15107-15109	IN	denotes	in
T8573	15110-15113	DT	denotes	the
T8574	15114-15118	NN	denotes	size
T8575	15119-15121	IN	denotes	of
T8576	15122-15125	DT	denotes	the
T8578	15126-15132	JJ	denotes	aortic
T8577	15133-15136	NN	denotes	sac
T8579	15136-15137	.	denotes	.
T8580	15137-15468	sentence	denotes	Histological analysis of control samples displayed the characteristic rotation of the aortic sac and truncal OFT at the level where the AP septation takes place and verified the presence of the distinctive condensed AP-septal mesenchyme, which gradually divided the OFT to the aorta and the pulmonary trunk (asterisks in Fig. 6A).
T8581	15138-15150	JJ	denotes	Histological
T8582	15151-15159	NN	denotes	analysis
T8584	15160-15162	IN	denotes	of
T8585	15163-15170	NN	denotes	control
T8586	15171-15178	NNS	denotes	samples
T8583	15179-15188	VBD	denotes	displayed
T8587	15189-15192	DT	denotes	the
T8589	15193-15207	JJ	denotes	characteristic
T8588	15208-15216	NN	denotes	rotation
T8590	15217-15219	IN	denotes	of
T8591	15220-15223	DT	denotes	the
T8593	15224-15230	JJ	denotes	aortic
T8592	15231-15234	NN	denotes	sac
T8594	15235-15238	CC	denotes	and
T8595	15239-15246	JJ	denotes	truncal
T8596	15247-15250	NN	denotes	OFT
T8597	15251-15253	IN	denotes	at
T8598	15254-15257	DT	denotes	the
T8599	15258-15263	NN	denotes	level
T8600	15264-15269	WRB	denotes	where
T8602	15270-15273	DT	denotes	the
T8604	15274-15276	NN	denotes	AP
T8603	15277-15286	NN	denotes	septation
T8601	15287-15292	VBZ	denotes	takes
T8605	15293-15298	NN	denotes	place
T8606	15299-15302	CC	denotes	and
T8607	15303-15311	VBD	denotes	verified
T8608	15312-15315	DT	denotes	the
T8609	15316-15324	NN	denotes	presence
T8610	15325-15327	IN	denotes	of
T8611	15328-15331	DT	denotes	the
T8613	15332-15343	JJ	denotes	distinctive
T8614	15344-15353	VBN	denotes	condensed
T8615	15354-15356	NN	denotes	AP
T8616	15356-15357	HYPH	denotes	-
T8617	15357-15363	JJ	denotes	septal
T8612	15364-15374	NN	denotes	mesenchyme
T8618	15374-15376	,	denotes	,
T8619	15376-15381	WDT	denotes	which
T8621	15382-15391	RB	denotes	gradually
T8620	15392-15399	VBD	denotes	divided
T8622	15400-15403	DT	denotes	the
T8623	15404-15407	NN	denotes	OFT
T8624	15408-15410	IN	denotes	to
T8625	15411-15414	DT	denotes	the
T8626	15415-15420	NN	denotes	aorta
T8627	15421-15424	CC	denotes	and
T8628	15425-15428	DT	denotes	the
T8630	15429-15438	JJ	denotes	pulmonary
T8629	15439-15444	NN	denotes	trunk
T8631	15445-15446	-LRB-	denotes	(
T8632	15446-15455	NNS	denotes	asterisks
T8633	15456-15458	IN	denotes	in
T8634	15459-15463	NN	denotes	Fig.
T8635	15464-15466	NN	denotes	6A
T8636	15466-15467	-RRB-	denotes	)
T8637	15467-15468	.	denotes	.
T8638	15468-15620	sentence	denotes	R26R lineage tracing showed that this tissue is derived from the NC, while immunostaining for α-SMA showed differentiation into smooth muscle (Fig 6B).
T8639	15469-15473	NN	denotes	R26R
T8641	15474-15481	NN	denotes	lineage
T8640	15482-15489	NN	denotes	tracing
T8642	15490-15496	VBD	denotes	showed
T8643	15497-15501	IN	denotes	that
T8645	15502-15506	DT	denotes	this
T8646	15507-15513	NN	denotes	tissue
T8647	15514-15516	VBZ	denotes	is
T8644	15517-15524	VBN	denotes	derived
T8648	15525-15529	IN	denotes	from
T8649	15530-15533	DT	denotes	the
T8650	15534-15536	NN	denotes	NC
T8651	15536-15538	,	denotes	,
T8652	15538-15543	IN	denotes	while
T8654	15544-15558	NN	denotes	immunostaining
T8655	15559-15562	IN	denotes	for
T8656	15563-15564	NN	denotes	α
T8658	15564-15565	HYPH	denotes	-
T8657	15565-15568	NN	denotes	SMA
T8653	15569-15575	VBD	denotes	showed
T8659	15576-15591	NN	denotes	differentiation
T8660	15592-15596	IN	denotes	into
T8661	15597-15603	JJ	denotes	smooth
T8662	15604-15610	NN	denotes	muscle
T8663	15611-15612	-LRB-	denotes	(
T8665	15612-15615	NN	denotes	Fig
T8664	15616-15618	NN	denotes	6B
T8666	15618-15619	-RRB-	denotes	)
T8667	15619-15620	.	denotes	.
T8668	15620-15804	sentence	denotes	In Alk5/Wnt1-Cre mutants the characteristic rotation of the aortic sac and truncal OFT fails to take place (Fig. 6G–L), and a properly formed AP-septum was not detectable (Fig. 6G,H).
T8669	15621-15623	IN	denotes	In
T8671	15624-15628	NN	denotes	Alk5
T8673	15628-15629	HYPH	denotes	/
T8674	15629-15633	NN	denotes	Wnt1
T8675	15633-15634	HYPH	denotes	-
T8672	15634-15637	NN	denotes	Cre
T8676	15638-15645	NNS	denotes	mutants
T8677	15646-15649	DT	denotes	the
T8679	15650-15664	JJ	denotes	characteristic
T8678	15665-15673	NN	denotes	rotation
T8680	15674-15676	IN	denotes	of
T8681	15677-15680	DT	denotes	the
T8683	15681-15687	JJ	denotes	aortic
T8682	15688-15691	NN	denotes	sac
T8684	15692-15695	CC	denotes	and
T8685	15696-15703	JJ	denotes	truncal
T8686	15704-15707	NN	denotes	OFT
T8670	15708-15713	VBZ	denotes	fails
T8687	15714-15716	TO	denotes	to
T8688	15717-15721	VB	denotes	take
T8689	15722-15727	NN	denotes	place
T8690	15728-15729	-LRB-	denotes	(
T8692	15729-15733	NN	denotes	Fig.
T8691	15734-15736	NN	denotes	6G
T8693	15736-15737	SYM	denotes	–
T8694	15737-15738	NN	denotes	L
T8695	15738-15739	-RRB-	denotes	)
T8696	15739-15741	,	denotes	,
T8697	15741-15744	CC	denotes	and
T8698	15745-15746	DT	denotes	a
T8700	15747-15755	RB	denotes	properly
T8701	15756-15762	VBN	denotes	formed
T8702	15763-15765	NN	denotes	AP
T8703	15765-15766	HYPH	denotes	-
T8699	15766-15772	NN	denotes	septum
T8704	15773-15776	VBD	denotes	was
T8705	15777-15780	RB	denotes	not
T8706	15781-15791	JJ	denotes	detectable
T8707	15792-15793	-LRB-	denotes	(
T8709	15793-15797	NN	denotes	Fig.
T8710	15798-15800	NN	denotes	6G
T8711	15800-15801	,	denotes	,
T8708	15801-15802	NN	denotes	H
T8712	15802-15803	-RRB-	denotes	)
T8713	15803-15804	.	denotes	.
T8714	15804-15912	sentence	denotes	R26R lineage tracing demonstrated that the defects were not due to failure of NCCs to reach the OFT region.
T8715	15805-15809	NN	denotes	R26R
T8717	15810-15817	NN	denotes	lineage
T8716	15818-15825	NN	denotes	tracing
T8718	15826-15838	VBD	denotes	demonstrated
T8719	15839-15843	IN	denotes	that
T8721	15844-15847	DT	denotes	the
T8722	15848-15855	NNS	denotes	defects
T8720	15856-15860	VBD	denotes	were
T8723	15861-15864	RB	denotes	not
T8724	15865-15868	IN	denotes	due
T8725	15869-15871	IN	denotes	to
T8726	15872-15879	NN	denotes	failure
T8727	15880-15882	IN	denotes	of
T8728	15883-15887	NNS	denotes	NCCs
T8729	15888-15890	TO	denotes	to
T8730	15891-15896	VB	denotes	reach
T8731	15897-15900	DT	denotes	the
T8733	15901-15904	NN	denotes	OFT
T8732	15905-15911	NN	denotes	region
T8734	15911-15912	.	denotes	.
T8735	15912-16070	sentence	denotes	NC-derived cells around the abnomally bifurcated aortic sac, the abnormally large sixth PAAs and the truncus demonstrated strong αSMA staining (Fig. 6H,J,L).
T8736	15913-15915	NN	denotes	NC
T8738	15915-15916	HYPH	denotes	-
T8737	15916-15923	VBN	denotes	derived
T8739	15924-15929	NNS	denotes	cells
T8741	15930-15936	IN	denotes	around
T8742	15937-15940	DT	denotes	the
T8744	15941-15950	RB	denotes	abnomally
T8745	15951-15961	VBN	denotes	bifurcated
T8746	15962-15968	JJ	denotes	aortic
T8743	15969-15972	NN	denotes	sac
T8747	15972-15974	,	denotes	,
T8748	15974-15977	DT	denotes	the
T8750	15978-15988	RB	denotes	abnormally
T8751	15989-15994	JJ	denotes	large
T8752	15995-16000	JJ	denotes	sixth
T8749	16001-16005	NNS	denotes	PAAs
T8753	16006-16009	CC	denotes	and
T8754	16010-16013	DT	denotes	the
T8755	16014-16021	NN	denotes	truncus
T8740	16022-16034	VBD	denotes	demonstrated
T8756	16035-16041	JJ	denotes	strong
T8758	16042-16046	NN	denotes	αSMA
T8757	16047-16055	NN	denotes	staining
T8759	16056-16057	-LRB-	denotes	(
T8761	16057-16061	NN	denotes	Fig.
T8762	16062-16064	NN	denotes	6H
T8763	16064-16065	,	denotes	,
T8764	16065-16066	NN	denotes	J
T8765	16066-16067	,	denotes	,
T8760	16067-16068	NN	denotes	L
T8766	16068-16069	-RRB-	denotes	)
T8767	16069-16070	.	denotes	.
T8768	16070-16183	sentence	denotes	Recently, we showed that the NC-specific mutants of the related type I receptor, Alk2, display PTA as well [12].
T8769	16071-16079	RB	denotes	Recently
T8771	16079-16081	,	denotes	,
T8772	16081-16083	PRP	denotes	we
T8770	16084-16090	VBD	denotes	showed
T8773	16091-16095	IN	denotes	that
T8775	16096-16099	DT	denotes	the
T8777	16100-16102	NN	denotes	NC
T8779	16102-16103	HYPH	denotes	-
T8778	16103-16111	JJ	denotes	specific
T8776	16112-16119	NNS	denotes	mutants
T8780	16120-16122	IN	denotes	of
T8781	16123-16126	DT	denotes	the
T8783	16127-16134	VBN	denotes	related
T8784	16135-16139	NN	denotes	type
T8785	16140-16141	CD	denotes	I
T8782	16142-16150	NN	denotes	receptor
T8786	16150-16152	,	denotes	,
T8787	16152-16156	NN	denotes	Alk2
T8788	16156-16158	,	denotes	,
T8774	16158-16165	VBP	denotes	display
T8789	16166-16169	NN	denotes	PTA
T8790	16170-16172	RB	denotes	as
T8791	16173-16177	RB	denotes	well
T8792	16178-16179	-LRB-	denotes	[
T8793	16179-16181	CD	denotes	12
T8794	16181-16182	-RRB-	denotes	]
T8795	16182-16183	.	denotes	.
T8796	16183-16318	sentence	denotes	In Alk2/Wnt1-Cre mutants, the rotation of the aortic sac and truncal OFT failed to occur (Fig. 6M–R) as seen in Alk5/Wnt1-Cre mutants.
T8797	16184-16186	IN	denotes	In
T8799	16187-16191	NN	denotes	Alk2
T8801	16191-16192	HYPH	denotes	/
T8802	16192-16196	NN	denotes	Wnt1
T8803	16196-16197	HYPH	denotes	-
T8800	16197-16200	NN	denotes	Cre
T8804	16201-16208	NNS	denotes	mutants
T8805	16208-16210	,	denotes	,
T8806	16210-16213	DT	denotes	the
T8807	16214-16222	NN	denotes	rotation
T8808	16223-16225	IN	denotes	of
T8809	16226-16229	DT	denotes	the
T8811	16230-16236	JJ	denotes	aortic
T8810	16237-16240	NN	denotes	sac
T8812	16241-16244	CC	denotes	and
T8813	16245-16252	JJ	denotes	truncal
T8814	16253-16256	NN	denotes	OFT
T8798	16257-16263	VBD	denotes	failed
T8815	16264-16266	TO	denotes	to
T8816	16267-16272	VB	denotes	occur
T8817	16273-16274	-LRB-	denotes	(
T8819	16274-16278	NN	denotes	Fig.
T8818	16279-16281	NN	denotes	6M
T8820	16281-16282	SYM	denotes	–
T8821	16282-16283	NN	denotes	R
T8822	16283-16284	-RRB-	denotes	)
T8823	16285-16287	IN	denotes	as
T8824	16288-16292	VBN	denotes	seen
T8825	16293-16295	IN	denotes	in
T8826	16296-16300	NN	denotes	Alk5
T8828	16300-16301	HYPH	denotes	/
T8829	16301-16305	NN	denotes	Wnt1
T8830	16305-16306	HYPH	denotes	-
T8827	16306-16309	NN	denotes	Cre
T8831	16310-16317	NNS	denotes	mutants
T8832	16317-16318	.	denotes	.
T8833	16318-16644	sentence	denotes	However, in Alk2 mutants the 6th pair of the PAAs was grossly hypoplastic, and while the Alk2/Wnt1-Cre mutants displayed a noticeable amount of septal tissue between the 4th and 6th PAAs (Fig. 6M,N), the condensed septal mesenchyme lacking Alk2 failed to extend the prongs into the truncal cushions and to form the AP septum.
T8834	16319-16326	RB	denotes	However
T8836	16326-16328	,	denotes	,
T8837	16328-16330	IN	denotes	in
T8838	16331-16335	NN	denotes	Alk2
T8839	16336-16343	NNS	denotes	mutants
T8840	16344-16347	DT	denotes	the
T8842	16348-16351	JJ	denotes	6th
T8841	16352-16356	NN	denotes	pair
T8843	16357-16359	IN	denotes	of
T8844	16360-16363	DT	denotes	the
T8845	16364-16368	NNS	denotes	PAAs
T8835	16369-16372	VBD	denotes	was
T8846	16373-16380	RB	denotes	grossly
T8847	16381-16392	JJ	denotes	hypoplastic
T8848	16392-16394	,	denotes	,
T8849	16394-16397	CC	denotes	and
T8850	16398-16403	IN	denotes	while
T8852	16404-16407	DT	denotes	the
T8854	16408-16412	NN	denotes	Alk2
T8856	16412-16413	HYPH	denotes	/
T8857	16413-16417	NN	denotes	Wnt1
T8858	16417-16418	HYPH	denotes	-
T8855	16418-16421	NN	denotes	Cre
T8853	16422-16429	NNS	denotes	mutants
T8851	16430-16439	VBD	denotes	displayed
T8860	16440-16441	DT	denotes	a
T8862	16442-16452	JJ	denotes	noticeable
T8861	16453-16459	NN	denotes	amount
T8863	16460-16462	IN	denotes	of
T8864	16463-16469	JJ	denotes	septal
T8865	16470-16476	NN	denotes	tissue
T8866	16477-16484	IN	denotes	between
T8867	16485-16488	DT	denotes	the
T8869	16489-16492	JJ	denotes	4th
T8870	16493-16496	CC	denotes	and
T8871	16497-16500	JJ	denotes	6th
T8868	16501-16505	NNS	denotes	PAAs
T8872	16506-16507	-LRB-	denotes	(
T8874	16507-16511	NN	denotes	Fig.
T8875	16512-16514	NN	denotes	6M
T8876	16514-16515	,	denotes	,
T8873	16515-16516	NN	denotes	N
T8877	16516-16517	-RRB-	denotes	)
T8878	16517-16519	,	denotes	,
T8879	16519-16522	DT	denotes	the
T8881	16523-16532	VBN	denotes	condensed
T8882	16533-16539	JJ	denotes	septal
T8880	16540-16550	NN	denotes	mesenchyme
T8883	16551-16558	VBG	denotes	lacking
T8884	16559-16563	NN	denotes	Alk2
T8859	16564-16570	VBD	denotes	failed
T8885	16571-16573	TO	denotes	to
T8886	16574-16580	VB	denotes	extend
T8887	16581-16584	DT	denotes	the
T8888	16585-16591	NNS	denotes	prongs
T8889	16592-16596	IN	denotes	into
T8890	16597-16600	DT	denotes	the
T8892	16601-16608	JJ	denotes	truncal
T8891	16609-16617	NNS	denotes	cushions
T8893	16618-16621	CC	denotes	and
T8894	16622-16624	TO	denotes	to
T8895	16625-16629	VB	denotes	form
T8896	16630-16633	DT	denotes	the
T8898	16634-16636	NN	denotes	AP
T8897	16637-16643	NN	denotes	septum
T8899	16643-16644	.	denotes	.
T8900	16644-16775	sentence	denotes	Concurrently, the 6th PAAs were losing their patency, which may have further contributed to the failed AP septation (Fig. 6M,O,Q).
T8901	16645-16657	RB	denotes	Concurrently
T8903	16657-16659	,	denotes	,
T8904	16659-16662	DT	denotes	the
T8906	16663-16666	JJ	denotes	6th
T8905	16667-16671	NNS	denotes	PAAs
T8907	16672-16676	VBD	denotes	were
T8902	16677-16683	VBG	denotes	losing
T8908	16684-16689	PRP$	denotes	their
T8909	16690-16697	NN	denotes	patency
T8910	16697-16699	,	denotes	,
T8911	16699-16704	WDT	denotes	which
T8913	16705-16708	MD	denotes	may
T8914	16709-16713	VB	denotes	have
T8915	16714-16721	RB	denotes	further
T8912	16722-16733	VBN	denotes	contributed
T8916	16734-16736	IN	denotes	to
T8917	16737-16740	DT	denotes	the
T8919	16741-16747	VBN	denotes	failed
T8920	16748-16750	NN	denotes	AP
T8918	16751-16760	NN	denotes	septation
T8921	16761-16762	-LRB-	denotes	(
T8923	16762-16766	NN	denotes	Fig.
T8924	16767-16769	NN	denotes	6M
T8925	16769-16770	,	denotes	,
T8926	16770-16771	NN	denotes	O
T8927	16771-16772	,	denotes	,
T8922	16772-16773	NN	denotes	Q
T8928	16773-16774	-RRB-	denotes	)
T8929	16774-16775	.	denotes	.
T8930	16775-17076	sentence	denotes	While CNCCs managed to migrate to the aortic sac and the truncal cushion level (Fig. 6N,P,R), immunostaining for αSMA appeared much weaker when compared to controls and Alk5 mutants, implying that ALK2-mediated signaling is involved in smooth muscle cell differentiation as previously suggested [12].
T8931	16776-16781	IN	denotes	While
T8933	16782-16787	NNS	denotes	CNCCs
T8932	16788-16795	VBD	denotes	managed
T8935	16796-16798	TO	denotes	to
T8936	16799-16806	VB	denotes	migrate
T8937	16807-16809	IN	denotes	to
T8938	16810-16813	DT	denotes	the
T8940	16814-16820	JJ	denotes	aortic
T8939	16821-16824	NN	denotes	sac
T8941	16825-16828	CC	denotes	and
T8942	16829-16832	DT	denotes	the
T8944	16833-16840	JJ	denotes	truncal
T8945	16841-16848	NN	denotes	cushion
T8943	16849-16854	NN	denotes	level
T8946	16855-16856	-LRB-	denotes	(
T8948	16856-16860	NN	denotes	Fig.
T8949	16861-16863	NN	denotes	6N
T8950	16863-16864	,	denotes	,
T8951	16864-16865	NN	denotes	P
T8952	16865-16866	,	denotes	,
T8947	16866-16867	NN	denotes	R
T8953	16867-16868	-RRB-	denotes	)
T8954	16868-16870	,	denotes	,
T8955	16870-16884	NN	denotes	immunostaining
T8956	16885-16888	IN	denotes	for
T8957	16889-16893	NN	denotes	αSMA
T8934	16894-16902	VBD	denotes	appeared
T8958	16903-16907	RB	denotes	much
T8959	16908-16914	JJR	denotes	weaker
T8960	16915-16919	WRB	denotes	when
T8961	16920-16928	VBN	denotes	compared
T8962	16929-16931	IN	denotes	to
T8963	16932-16940	NNS	denotes	controls
T8964	16941-16944	CC	denotes	and
T8965	16945-16949	NN	denotes	Alk5
T8966	16950-16957	NNS	denotes	mutants
T8967	16957-16959	,	denotes	,
T8968	16959-16967	VBG	denotes	implying
T8969	16968-16972	IN	denotes	that
T8971	16973-16977	NN	denotes	ALK2
T8973	16977-16978	HYPH	denotes	-
T8972	16978-16986	VBN	denotes	mediated
T8974	16987-16996	NN	denotes	signaling
T8975	16997-16999	VBZ	denotes	is
T8970	17000-17008	VBN	denotes	involved
T8976	17009-17011	IN	denotes	in
T8977	17012-17018	JJ	denotes	smooth
T8978	17019-17025	NN	denotes	muscle
T8980	17026-17030	NN	denotes	cell
T8979	17031-17046	NN	denotes	differentiation
T8981	17047-17049	IN	denotes	as
T8983	17050-17060	RB	denotes	previously
T8982	17061-17070	VBN	denotes	suggested
T8984	17071-17072	-LRB-	denotes	[
T8985	17072-17074	CD	denotes	12
T8986	17074-17075	-RRB-	denotes	]
T8987	17075-17076	.	denotes	.
T8988	17076-17321	sentence	denotes	To conclude, while both Alk2 and Alk5 mutants demonstrate a failure in both the rotation of the aortic sac and the truncal OFT, and in the formation of the AP septum, the pathogenetic mechanisms behind these defects appear remarkably different.
T8989	17077-17079	TO	denotes	To
T8990	17080-17088	VB	denotes	conclude
T8992	17088-17090	,	denotes	,
T8993	17090-17095	IN	denotes	while
T8995	17096-17100	CC	denotes	both
T8996	17101-17105	NN	denotes	Alk2
T8998	17106-17109	CC	denotes	and
T8999	17110-17114	NN	denotes	Alk5
T8997	17115-17122	NNS	denotes	mutants
T8994	17123-17134	VBP	denotes	demonstrate
T9000	17135-17136	DT	denotes	a
T9001	17137-17144	NN	denotes	failure
T9002	17145-17147	IN	denotes	in
T9003	17148-17152	CC	denotes	both
T9005	17153-17156	DT	denotes	the
T9004	17157-17165	NN	denotes	rotation
T9006	17166-17168	IN	denotes	of
T9007	17169-17172	DT	denotes	the
T9009	17173-17179	JJ	denotes	aortic
T9008	17180-17183	NN	denotes	sac
T9010	17184-17187	CC	denotes	and
T9011	17188-17191	DT	denotes	the
T9013	17192-17199	JJ	denotes	truncal
T9012	17200-17203	NN	denotes	OFT
T9014	17203-17205	,	denotes	,
T9015	17205-17208	CC	denotes	and
T9016	17209-17211	IN	denotes	in
T9017	17212-17215	DT	denotes	the
T9018	17216-17225	NN	denotes	formation
T9019	17226-17228	IN	denotes	of
T9020	17229-17232	DT	denotes	the
T9022	17233-17235	NN	denotes	AP
T9021	17236-17242	NN	denotes	septum
T9023	17242-17244	,	denotes	,
T9024	17244-17247	DT	denotes	the
T9026	17248-17260	JJ	denotes	pathogenetic
T9025	17261-17271	NNS	denotes	mechanisms
T9027	17272-17278	IN	denotes	behind
T9028	17279-17284	DT	denotes	these
T9029	17285-17292	NNS	denotes	defects
T8991	17293-17299	VBP	denotes	appear
T9030	17300-17310	RB	denotes	remarkably
T9031	17311-17320	JJ	denotes	different
T9032	17320-17321	.	denotes	.
T19235	17332-17335	DT	denotes	The
T19237	17336-17343	JJ	denotes	truncal
T19236	17344-17347	NN	denotes	OFT
T19238	17348-17353	VBZ	denotes	fails
T19239	17354-17356	TO	denotes	to
T19240	17357-17363	VB	denotes	rotate
T19241	17364-17366	IN	denotes	in
T19242	17367-17371	NN	denotes	Alk5
T19244	17371-17372	HYPH	denotes	/
T19245	17372-17376	NN	denotes	Wnt1
T19246	17376-17377	HYPH	denotes	-
T19243	17377-17380	NN	denotes	Cre
T19247	17381-17388	NNS	denotes	mutants
T19248	17388-17389	.	denotes	.
T19249	17389-17652	sentence	denotes	Left (A, C) and right (B, D) lateral images of ink-injected control (A-B) and mutant (C-D) embryos at E11.5 demonstrate the abnormally straight OFT in mutants lacking the typical conotruncal transition (black arrow in A vs. black arrowhead in C) seen in control.
T19250	17390-17394	JJ	denotes	Left
T19252	17395-17396	-LRB-	denotes	(
T19254	17396-17397	NN	denotes	A
T19255	17397-17399	,	denotes	,
T19253	17399-17400	NN	denotes	C
T19256	17400-17401	-RRB-	denotes	)
T19257	17402-17405	CC	denotes	and
T19258	17406-17411	JJ	denotes	right
T19259	17412-17413	-LRB-	denotes	(
T19261	17413-17414	NN	denotes	B
T19262	17414-17416	,	denotes	,
T19260	17416-17417	NN	denotes	D
T19263	17417-17418	-RRB-	denotes	)
T19264	17419-17426	JJ	denotes	lateral
T19251	17427-17433	NNS	denotes	images
T19266	17434-17436	IN	denotes	of
T19267	17437-17440	NN	denotes	ink
T19269	17440-17441	HYPH	denotes	-
T19268	17441-17449	VBN	denotes	injected
T19271	17450-17457	NN	denotes	control
T19272	17458-17459	-LRB-	denotes	(
T19273	17459-17460	NN	denotes	A
T19274	17460-17461	SYM	denotes	-
T19275	17461-17462	NN	denotes	B
T19276	17462-17463	-RRB-	denotes	)
T19277	17464-17467	CC	denotes	and
T19278	17468-17474	NN	denotes	mutant
T19279	17475-17476	-LRB-	denotes	(
T19280	17476-17477	NN	denotes	C
T19281	17477-17478	SYM	denotes	-
T19282	17478-17479	NN	denotes	D
T19283	17479-17480	-RRB-	denotes	)
T19270	17481-17488	NNS	denotes	embryos
T19284	17489-17491	IN	denotes	at
T19285	17492-17497	NN	denotes	E11.5
T19265	17498-17509	VBP	denotes	demonstrate
T19286	17510-17513	DT	denotes	the
T19288	17514-17524	RB	denotes	abnormally
T19289	17525-17533	JJ	denotes	straight
T19287	17534-17537	NN	denotes	OFT
T19290	17538-17540	IN	denotes	in
T19291	17541-17548	NNS	denotes	mutants
T19292	17549-17556	VBG	denotes	lacking
T19293	17557-17560	DT	denotes	the
T19295	17561-17568	JJ	denotes	typical
T19296	17569-17580	JJ	denotes	conotruncal
T19294	17581-17591	NN	denotes	transition
T19297	17592-17593	-LRB-	denotes	(
T19299	17593-17598	JJ	denotes	black
T19298	17599-17604	NN	denotes	arrow
T19300	17605-17607	IN	denotes	in
T19301	17608-17609	NN	denotes	A
T19302	17610-17613	CC	denotes	vs.
T19303	17614-17619	JJ	denotes	black
T19304	17620-17629	NN	denotes	arrowhead
T19305	17630-17632	IN	denotes	in
T19306	17633-17634	NN	denotes	C
T19307	17634-17635	-RRB-	denotes	)
T19308	17636-17640	VBN	denotes	seen
T19309	17641-17643	IN	denotes	in
T19310	17644-17651	NN	denotes	control
T19311	17651-17652	.	denotes	.
T19312	17652-17714	sentence	denotes	Red arrowhead (C) points to the abnormally shaped aortic sac.
T19313	17653-17656	JJ	denotes	Red
T19314	17657-17666	NN	denotes	arrowhead
T19316	17667-17668	-LRB-	denotes	(
T19317	17668-17669	NN	denotes	C
T19318	17669-17670	-RRB-	denotes	)
T19315	17671-17677	VBZ	denotes	points
T19319	17678-17680	IN	denotes	to
T19320	17681-17684	DT	denotes	the
T19322	17685-17695	RB	denotes	abnormally
T19323	17696-17702	VBN	denotes	shaped
T19324	17703-17709	JJ	denotes	aortic
T19321	17710-17713	NN	denotes	sac
T19325	17713-17714	.	denotes	.
T19326	17714-17757	sentence	denotes	Red "s", aortic sac; t, truncus; c, conus.
T19327	17715-17718	JJ	denotes	Red
T19329	17719-17720	``	denotes	"
T19328	17720-17721	NN	denotes	s
T19330	17721-17722	''	denotes	"
T19331	17722-17724	,	denotes	,
T19332	17724-17730	JJ	denotes	aortic
T19333	17731-17734	NN	denotes	sac
T19334	17734-17735	:	denotes	;
T19335	17736-17737	NN	denotes	t
T19336	17737-17739	,	denotes	,
T19337	17739-17746	NN	denotes	truncus
T19338	17746-17747	:	denotes	;
T19339	17748-17749	NN	denotes	c
T19340	17749-17751	,	denotes	,
T19341	17751-17756	NN	denotes	conus
T19342	17756-17757	.	denotes	.
T19663	17768-17777	NN	denotes	Signaling
T19665	17778-17781	IN	denotes	via
T19666	17782-17786	NN	denotes	ALK5
T19667	17787-17790	CC	denotes	and
T19668	17791-17795	NN	denotes	ALK2
T19664	17796-17804	VBZ	denotes	controls
T19669	17805-17814	JJ	denotes	different
T19670	17815-17822	NNS	denotes	aspects
T19671	17823-17825	IN	denotes	of
T19672	17826-17833	JJ	denotes	aortico
T19674	17833-17834	HYPH	denotes	-
T19673	17834-17843	JJ	denotes	pulmonary
T19675	17844-17853	NN	denotes	septation
T19676	17853-17854	.	denotes	.
T19677	17854-18015	sentence	denotes	Frontal sections from distal (top row) to proximal (bottom row) of the control (A-F), Alk5/Wnt1-Cre mutant (G-L) and Alk2/Wnt1-Cre mutant (M-R) samples (E11.5).
T19678	17855-17862	JJ	denotes	Frontal
T19679	17863-17871	NNS	denotes	sections
T19680	17872-17876	IN	denotes	from
T19681	17877-17883	JJ	denotes	distal
T19682	17884-17885	-LRB-	denotes	(
T19684	17885-17888	JJ	denotes	top
T19683	17889-17892	NN	denotes	row
T19685	17892-17893	-RRB-	denotes	)
T19686	17894-17896	IN	denotes	to
T19687	17897-17905	JJ	denotes	proximal
T19688	17906-17907	-LRB-	denotes	(
T19690	17907-17913	JJ	denotes	bottom
T19689	17914-17917	NN	denotes	row
T19691	17917-17918	-RRB-	denotes	)
T19692	17919-17921	IN	denotes	of
T19693	17922-17925	DT	denotes	the
T19695	17926-17933	NN	denotes	control
T19696	17934-17935	-LRB-	denotes	(
T19697	17935-17936	NN	denotes	A
T19698	17936-17937	SYM	denotes	-
T19699	17937-17938	NN	denotes	F
T19700	17938-17939	-RRB-	denotes	)
T19701	17939-17941	,	denotes	,
T19702	17941-17945	NN	denotes	Alk5
T19704	17945-17946	HYPH	denotes	/
T19705	17946-17950	NN	denotes	Wnt1
T19706	17950-17951	HYPH	denotes	-
T19703	17951-17954	NN	denotes	Cre
T19707	17955-17961	NN	denotes	mutant
T19708	17962-17963	-LRB-	denotes	(
T19709	17963-17964	NN	denotes	G
T19710	17964-17965	SYM	denotes	-
T19711	17965-17966	NN	denotes	L
T19712	17966-17967	-RRB-	denotes	)
T19713	17968-17971	CC	denotes	and
T19714	17972-17976	NN	denotes	Alk2
T19716	17976-17977	HYPH	denotes	/
T19717	17977-17981	NN	denotes	Wnt1
T19718	17981-17982	HYPH	denotes	-
T19715	17982-17985	NN	denotes	Cre
T19719	17986-17992	NN	denotes	mutant
T19720	17993-17994	-LRB-	denotes	(
T19721	17994-17995	NN	denotes	M
T19722	17995-17996	SYM	denotes	-
T19723	17996-17997	NN	denotes	R
T19724	17997-17998	-RRB-	denotes	)
T19694	17999-18006	NNS	denotes	samples
T19725	18007-18008	-LRB-	denotes	(
T19726	18008-18013	NN	denotes	E11.5
T19727	18013-18014	-RRB-	denotes	)
T19728	18014-18015	.	denotes	.
T19729	18015-18166	sentence	denotes	A, C, E, G, I, K, M, O, Q, H&E staining; B, D, F, H, J, L, N, P, R, double staining for αSMA (brown) and β-galactosidase (green; R26R reporter assay).
T19730	18016-18017	LS	denotes	A
T19732	18017-18019	,	denotes	,
T19733	18019-18020	LS	denotes	C
T19734	18020-18022	,	denotes	,
T19735	18022-18023	LS	denotes	E
T19736	18023-18025	,	denotes	,
T19737	18025-18026	LS	denotes	G
T19738	18026-18028	,	denotes	,
T19739	18028-18029	LS	denotes	I
T19740	18029-18031	,	denotes	,
T19741	18031-18032	LS	denotes	K
T19742	18032-18034	,	denotes	,
T19743	18034-18035	LS	denotes	M
T19744	18035-18037	,	denotes	,
T19745	18037-18038	LS	denotes	O
T19746	18038-18040	,	denotes	,
T19747	18040-18041	LS	denotes	Q
T19748	18041-18043	,	denotes	,
T19749	18043-18044	NN	denotes	H
T19750	18044-18045	CC	denotes	&
T19751	18045-18046	NN	denotes	E
T19731	18047-18055	NN	denotes	staining
T19752	18055-18056	:	denotes	;
T19753	18057-18058	LS	denotes	B
T19755	18058-18060	,	denotes	,
T19756	18060-18061	LS	denotes	D
T19757	18061-18063	,	denotes	,
T19758	18063-18064	LS	denotes	F
T19759	18064-18066	,	denotes	,
T19760	18066-18067	LS	denotes	H
T19761	18067-18069	,	denotes	,
T19762	18069-18070	LS	denotes	J
T19763	18070-18072	,	denotes	,
T19764	18072-18073	LS	denotes	L
T19765	18073-18075	,	denotes	,
T19766	18075-18076	LS	denotes	N
T19767	18076-18078	,	denotes	,
T19768	18078-18079	LS	denotes	P
T19769	18079-18081	,	denotes	,
T19770	18081-18082	LS	denotes	R
T19771	18082-18084	,	denotes	,
T19772	18084-18090	JJ	denotes	double
T19754	18091-18099	NN	denotes	staining
T19773	18100-18103	IN	denotes	for
T19774	18104-18108	NN	denotes	αSMA
T19775	18109-18110	-LRB-	denotes	(
T19776	18110-18115	JJ	denotes	brown
T19777	18115-18116	-RRB-	denotes	)
T19778	18117-18120	CC	denotes	and
T19779	18121-18122	NN	denotes	β
T19781	18122-18123	HYPH	denotes	-
T19780	18123-18136	NN	denotes	galactosidase
T19782	18137-18138	-LRB-	denotes	(
T19784	18138-18143	JJ	denotes	green
T19785	18143-18144	:	denotes	;
T19786	18145-18149	NN	denotes	R26R
T19787	18150-18158	NN	denotes	reporter
T19783	18159-18164	NN	denotes	assay
T19788	18164-18165	-RRB-	denotes	)
T19789	18165-18166	.	denotes	.
T19790	18166-18314	sentence	denotes	6, the 6th PAA; AS, aortic sac; TA, truncus arteriosus; Ao, Aorta; PT, pulmonary trunk; Asterisks in A, B, M and N depict the AP septal mesenchyme.
T19791	18167-18168	CD	denotes	6
T19793	18168-18170	,	denotes	,
T19794	18170-18173	DT	denotes	the
T19796	18174-18177	JJ	denotes	6th
T19795	18178-18181	NN	denotes	PAA
T19797	18181-18182	:	denotes	;
T19798	18183-18185	NN	denotes	AS
T19799	18185-18187	,	denotes	,
T19800	18187-18193	JJ	denotes	aortic
T19801	18194-18197	NN	denotes	sac
T19802	18197-18198	:	denotes	;
T19803	18199-18201	NN	denotes	TA
T19804	18201-18203	,	denotes	,
T19805	18203-18210	NN	denotes	truncus
T19806	18211-18221	NN	denotes	arteriosus
T19807	18221-18222	:	denotes	;
T19808	18223-18225	NN	denotes	Ao
T19809	18225-18227	,	denotes	,
T19810	18227-18232	NN	denotes	Aorta
T19811	18232-18233	:	denotes	;
T19812	18234-18236	NN	denotes	PT
T19813	18236-18238	,	denotes	,
T19814	18238-18247	JJ	denotes	pulmonary
T19815	18248-18253	NN	denotes	trunk
T19816	18253-18254	:	denotes	;
T19817	18255-18264	NNS	denotes	Asterisks
T19818	18265-18267	IN	denotes	in
T19819	18268-18269	NN	denotes	A
T19820	18269-18271	,	denotes	,
T19821	18271-18272	NN	denotes	B
T19822	18272-18274	,	denotes	,
T19823	18274-18275	NN	denotes	M
T19824	18276-18279	CC	denotes	and
T19825	18280-18281	NN	denotes	N
T19792	18282-18288	VBP	denotes	depict
T19826	18289-18292	DT	denotes	the
T19828	18293-18295	NN	denotes	AP
T19829	18296-18302	JJ	denotes	septal
T19827	18303-18313	NN	denotes	mesenchyme
T19830	18313-18314	.	denotes	.
T9837	18316-18320	NN	denotes	Alk5
T9839	18320-18321	HYPH	denotes	/
T9840	18321-18325	NN	denotes	Wnt1
T9841	18325-18326	HYPH	denotes	-
T9838	18326-18329	NN	denotes	Cre
T9842	18330-18337	NNS	denotes	mutants
T9843	18338-18345	VBP	denotes	display
T9844	18346-18355	VBN	denotes	increased
T9845	18356-18365	NN	denotes	apoptosis
T9846	18366-18368	IN	denotes	of
T9847	18369-18383	JJ	denotes	post-migratory
T9849	18384-18390	JJ	denotes	neural
T9850	18391-18396	NN	denotes	crest
T9848	18397-18402	NNS	denotes	cells
T9851	18402-18566	sentence	denotes	As described above, Alk5/Wnt1-Cre mutants displayed an inadequate amount of AP-septal tissue in the base of the aortic sac between the origins of 4th and 6th PAAs.
T9852	18403-18405	IN	denotes	As
T9853	18406-18415	VBN	denotes	described
T9855	18416-18421	RB	denotes	above
T9856	18421-18423	,	denotes	,
T9857	18423-18427	NN	denotes	Alk5
T9859	18427-18428	HYPH	denotes	/
T9860	18428-18432	NN	denotes	Wnt1
T9861	18432-18433	HYPH	denotes	-
T9858	18433-18436	NN	denotes	Cre
T9862	18437-18444	NNS	denotes	mutants
T9854	18445-18454	VBD	denotes	displayed
T9863	18455-18457	DT	denotes	an
T9865	18458-18468	JJ	denotes	inadequate
T9864	18469-18475	NN	denotes	amount
T9866	18476-18478	IN	denotes	of
T9867	18479-18481	NN	denotes	AP
T9869	18481-18482	HYPH	denotes	-
T9868	18482-18488	JJ	denotes	septal
T9870	18489-18495	NN	denotes	tissue
T9871	18496-18498	IN	denotes	in
T9872	18499-18502	DT	denotes	the
T9873	18503-18507	NN	denotes	base
T9874	18508-18510	IN	denotes	of
T9875	18511-18514	DT	denotes	the
T9877	18515-18521	JJ	denotes	aortic
T9876	18522-18525	NN	denotes	sac
T9878	18526-18533	IN	denotes	between
T9879	18534-18537	DT	denotes	the
T9880	18538-18545	NNS	denotes	origins
T9881	18546-18548	IN	denotes	of
T9882	18549-18552	JJ	denotes	4th
T9884	18553-18556	CC	denotes	and
T9885	18557-18560	JJ	denotes	6th
T9883	18561-18565	NNS	denotes	PAAs
T9886	18565-18566	.	denotes	.
T9887	18566-18725	sentence	denotes	To analyze whether this phenotype resulted either from defective CNCC proliferation or inappropriate apoptosis, we used BrdU and TUNEL staining, respectively.
T9888	18567-18569	TO	denotes	To
T9889	18570-18577	VB	denotes	analyze
T9891	18578-18585	IN	denotes	whether
T9893	18586-18590	DT	denotes	this
T9894	18591-18600	NN	denotes	phenotype
T9892	18601-18609	VBD	denotes	resulted
T9895	18610-18616	CC	denotes	either
T9896	18617-18621	IN	denotes	from
T9897	18622-18631	JJ	denotes	defective
T9899	18632-18636	NN	denotes	CNCC
T9898	18637-18650	NN	denotes	proliferation
T9900	18651-18653	CC	denotes	or
T9901	18654-18667	JJ	denotes	inappropriate
T9902	18668-18677	NN	denotes	apoptosis
T9903	18677-18679	,	denotes	,
T9904	18679-18681	PRP	denotes	we
T9890	18682-18686	VBD	denotes	used
T9905	18687-18691	NN	denotes	BrdU
T9907	18692-18695	CC	denotes	and
T9908	18696-18701	NN	denotes	TUNEL
T9906	18702-18710	NN	denotes	staining
T9909	18710-18712	,	denotes	,
T9910	18712-18724	RB	denotes	respectively
T9911	18724-18725	.	denotes	.
T9912	18725-18971	sentence	denotes	While CNCC proliferation was not affected in Alk5 mutants (data not shown), we could detect a dramatic increase in the number of TUNEL positive cells in tissues surrounding the aortic sac including the site where the AP-septum forms (Fig. 7A–C).
T9913	18726-18731	IN	denotes	While
T9915	18732-18736	NN	denotes	CNCC
T9916	18737-18750	NN	denotes	proliferation
T9917	18751-18754	VBD	denotes	was
T9918	18755-18758	RB	denotes	not
T9914	18759-18767	VBN	denotes	affected
T9920	18768-18770	IN	denotes	in
T9921	18771-18775	NN	denotes	Alk5
T9922	18776-18783	NNS	denotes	mutants
T9923	18784-18785	-LRB-	denotes	(
T9925	18785-18789	NNS	denotes	data
T9926	18790-18793	RB	denotes	not
T9924	18794-18799	VBN	denotes	shown
T9927	18799-18800	-RRB-	denotes	)
T9928	18800-18802	,	denotes	,
T9929	18802-18804	PRP	denotes	we
T9930	18805-18810	MD	denotes	could
T9919	18811-18817	VB	denotes	detect
T9931	18818-18819	DT	denotes	a
T9933	18820-18828	JJ	denotes	dramatic
T9932	18829-18837	NN	denotes	increase
T9934	18838-18840	IN	denotes	in
T9935	18841-18844	DT	denotes	the
T9936	18845-18851	NN	denotes	number
T9937	18852-18854	IN	denotes	of
T9938	18855-18860	NN	denotes	TUNEL
T9939	18861-18869	JJ	denotes	positive
T9940	18870-18875	NNS	denotes	cells
T9941	18876-18878	IN	denotes	in
T9942	18879-18886	NNS	denotes	tissues
T9943	18887-18898	VBG	denotes	surrounding
T9944	18899-18902	DT	denotes	the
T9946	18903-18909	JJ	denotes	aortic
T9945	18910-18913	NN	denotes	sac
T9947	18914-18923	VBG	denotes	including
T9948	18924-18927	DT	denotes	the
T9949	18928-18932	NN	denotes	site
T9950	18933-18938	WRB	denotes	where
T9952	18939-18942	DT	denotes	the
T9954	18943-18945	NN	denotes	AP
T9955	18945-18946	HYPH	denotes	-
T9953	18946-18952	NN	denotes	septum
T9951	18953-18958	VBZ	denotes	forms
T9956	18959-18960	-LRB-	denotes	(
T9958	18960-18964	NN	denotes	Fig.
T9957	18965-18967	NN	denotes	7A
T9959	18967-18968	SYM	denotes	–
T9960	18968-18969	NN	denotes	C
T9961	18969-18970	-RRB-	denotes	)
T9962	18970-18971	.	denotes	.
T9963	18971-19131	sentence	denotes	Dual staining for lacZ and TUNEL positive cells demonstrated that these cells were postmigratory CNCCs; this phenotype was already clearly detectable at E10.5.
T9964	18972-18976	JJ	denotes	Dual
T9965	18977-18985	NN	denotes	staining
T9967	18986-18989	IN	denotes	for
T9968	18990-18994	NN	denotes	lacZ
T9970	18995-18998	CC	denotes	and
T9971	18999-19004	NN	denotes	TUNEL
T9969	19005-19013	JJ	denotes	positive
T9972	19014-19019	NNS	denotes	cells
T9966	19020-19032	VBD	denotes	demonstrated
T9974	19033-19037	IN	denotes	that
T9976	19038-19043	DT	denotes	these
T9977	19044-19049	NNS	denotes	cells
T9975	19050-19054	VBD	denotes	were
T9978	19055-19068	JJ	denotes	postmigratory
T9979	19069-19074	NNS	denotes	CNCCs
T9980	19074-19075	:	denotes	;
T9981	19076-19080	DT	denotes	this
T9982	19081-19090	NN	denotes	phenotype
T9973	19091-19094	VBD	denotes	was
T9983	19095-19102	RB	denotes	already
T9984	19103-19110	RB	denotes	clearly
T9985	19111-19121	JJ	denotes	detectable
T9986	19122-19124	IN	denotes	at
T9987	19125-19130	NN	denotes	E10.5
T9988	19130-19131	.	denotes	.
T9989	19131-19249	sentence	denotes	These results were confirmed by using immunostaining for cleaved caspase-3, another marker for apoptosis (Fig. 7I,J).
T9990	19132-19137	DT	denotes	These
T9991	19138-19145	NNS	denotes	results
T9993	19146-19150	VBD	denotes	were
T9992	19151-19160	VBN	denotes	confirmed
T9994	19161-19163	IN	denotes	by
T9995	19164-19169	VBG	denotes	using
T9996	19170-19184	NN	denotes	immunostaining
T9997	19185-19188	IN	denotes	for
T9998	19189-19196	VBN	denotes	cleaved
T9999	19197-19204	NN	denotes	caspase
T10000	19204-19205	HYPH	denotes	-
T10001	19205-19206	CD	denotes	3
T10002	19206-19208	,	denotes	,
T10003	19208-19215	DT	denotes	another
T10004	19216-19222	NN	denotes	marker
T10005	19223-19226	IN	denotes	for
T10006	19227-19236	NN	denotes	apoptosis
T10007	19237-19238	-LRB-	denotes	(
T10009	19238-19242	NNP	denotes	Fig.
T10010	19243-19245	NN	denotes	7I
T10011	19245-19246	,	denotes	,
T10008	19246-19247	NN	denotes	J
T10012	19247-19248	-RRB-	denotes	)
T10013	19248-19249	.	denotes	.
T10014	19249-19420	sentence	denotes	In the chick, apoptotic neural crest-derived cells have also been found at the sites, where the prongs of the AP septum penetrate into the OFT cushion mesenchyme [25,26].
T10015	19250-19252	IN	denotes	In
T10017	19253-19256	DT	denotes	the
T10018	19257-19262	NN	denotes	chick
T10019	19262-19264	,	denotes	,
T10020	19264-19273	JJ	denotes	apoptotic
T10022	19274-19280	JJ	denotes	neural
T10023	19281-19286	NN	denotes	crest
T10025	19286-19287	HYPH	denotes	-
T10024	19287-19294	VBN	denotes	derived
T10021	19295-19300	NNS	denotes	cells
T10026	19301-19305	VBP	denotes	have
T10027	19306-19310	RB	denotes	also
T10028	19311-19315	VBN	denotes	been
T10016	19316-19321	VBN	denotes	found
T10029	19322-19324	IN	denotes	at
T10030	19325-19328	DT	denotes	the
T10031	19329-19334	NNS	denotes	sites
T10032	19334-19336	,	denotes	,
T10033	19336-19341	WRB	denotes	where
T10035	19342-19345	DT	denotes	the
T10036	19346-19352	NNS	denotes	prongs
T10037	19353-19355	IN	denotes	of
T10038	19356-19359	DT	denotes	the
T10040	19360-19362	NN	denotes	AP
T10039	19363-19369	NN	denotes	septum
T10034	19370-19379	VBP	denotes	penetrate
T10041	19380-19384	IN	denotes	into
T10042	19385-19388	DT	denotes	the
T10044	19389-19392	NN	denotes	OFT
T10045	19393-19400	NN	denotes	cushion
T10043	19401-19411	NN	denotes	mesenchyme
T10046	19412-19413	-LRB-	denotes	[
T10048	19413-19415	CD	denotes	25
T10049	19415-19416	,	denotes	,
T10047	19416-19418	CD	denotes	26
T10050	19418-19419	-RRB-	denotes	]
T10051	19419-19420	.	denotes	.
T10052	19420-19591	sentence	denotes	Thus, we compared apoptosis patterns also on the more proximal level, but found no detectable differences at E11.0 between Alk5/Wnt1-Cre mutants and controls (Fig. 7D,E).
T10053	19421-19425	RB	denotes	Thus
T10055	19425-19427	,	denotes	,
T10056	19427-19429	PRP	denotes	we
T10054	19430-19438	VBD	denotes	compared
T10057	19439-19448	NN	denotes	apoptosis
T10058	19449-19457	NNS	denotes	patterns
T10059	19458-19462	RB	denotes	also
T10060	19463-19465	IN	denotes	on
T10061	19466-19469	DT	denotes	the
T10063	19470-19474	RBR	denotes	more
T10064	19475-19483	JJ	denotes	proximal
T10062	19484-19489	NN	denotes	level
T10065	19489-19491	,	denotes	,
T10066	19491-19494	CC	denotes	but
T10067	19495-19500	VBD	denotes	found
T10068	19501-19503	DT	denotes	no
T10070	19504-19514	JJ	denotes	detectable
T10069	19515-19526	NNS	denotes	differences
T10071	19527-19529	IN	denotes	at
T10072	19530-19535	NN	denotes	E11.0
T10073	19536-19543	IN	denotes	between
T10074	19544-19548	NN	denotes	Alk5
T10076	19548-19549	HYPH	denotes	/
T10077	19549-19553	NN	denotes	Wnt1
T10078	19553-19554	HYPH	denotes	-
T10075	19554-19557	NN	denotes	Cre
T10079	19558-19565	NNS	denotes	mutants
T10080	19566-19569	CC	denotes	and
T10081	19570-19578	NNS	denotes	controls
T10082	19579-19580	-LRB-	denotes	(
T10084	19580-19584	NN	denotes	Fig.
T10085	19585-19587	NN	denotes	7D
T10086	19587-19588	,	denotes	,
T10083	19588-19589	NN	denotes	E
T10087	19589-19590	-RRB-	denotes	)
T10088	19590-19591	.	denotes	.
T10089	19591-19779	sentence	denotes	Unlike in Alk5/Wnt1-Cre mutant embryos, increased apoptosis of NC-derived cells is not responsible for the observed defects in the OFT septation in corresponding Alk2 mutants (Fig. 7C,E).
T10090	19592-19598	JJ	denotes	Unlike
T10092	19599-19601	IN	denotes	in
T10093	19602-19606	NN	denotes	Alk5
T10095	19606-19607	HYPH	denotes	/
T10096	19607-19611	NN	denotes	Wnt1
T10097	19611-19612	HYPH	denotes	-
T10094	19612-19615	NN	denotes	Cre
T10099	19616-19622	NN	denotes	mutant
T10098	19623-19630	NNS	denotes	embryos
T10100	19630-19632	,	denotes	,
T10101	19632-19641	VBN	denotes	increased
T10102	19642-19651	NN	denotes	apoptosis
T10103	19652-19654	IN	denotes	of
T10104	19655-19657	NN	denotes	NC
T10106	19657-19658	HYPH	denotes	-
T10105	19658-19665	VBN	denotes	derived
T10107	19666-19671	NNS	denotes	cells
T10091	19672-19674	VBZ	denotes	is
T10108	19675-19678	RB	denotes	not
T10109	19679-19690	JJ	denotes	responsible
T10110	19691-19694	IN	denotes	for
T10111	19695-19698	DT	denotes	the
T10113	19699-19707	VBN	denotes	observed
T10112	19708-19715	NNS	denotes	defects
T10114	19716-19718	IN	denotes	in
T10115	19719-19722	DT	denotes	the
T10117	19723-19726	NN	denotes	OFT
T10116	19727-19736	NN	denotes	septation
T10118	19737-19739	IN	denotes	in
T10119	19740-19753	VBG	denotes	corresponding
T10121	19754-19758	NN	denotes	Alk2
T10120	19759-19766	NNS	denotes	mutants
T10122	19767-19768	-LRB-	denotes	(
T10124	19768-19772	NN	denotes	Fig.
T10125	19773-19775	NN	denotes	7C
T10126	19775-19776	,	denotes	,
T10123	19776-19777	NN	denotes	E
T10127	19777-19778	-RRB-	denotes	)
T10128	19778-19779	.	denotes	.
T10129	19779-20628	sentence	denotes	Figure 7 Aberrant apoptosis of NCCs in Alk5/Wnt1-Cre mutants. TUNEL (A-H) and Cleaved Caspase-3 (I, J) staining at E11.0 demonstrates a notable increase in apoptosis in Alk5/Wnt1-Cre mutants (B, H, J) on the aortic sac level when compared to controls (A, G, I) or Alk2/Wnt1-Cre mutants (C) (frontal sections), while sections on the OFT level do not demonstrate differences between controls (D) and Alk5 (E) or Alk2 (F) mutants. G,H, TUNEL staining of lacZ-stained embryos demonstrates that apoptotic cells are of neural crest origin. G, control; H, mutant. AS, aortic sac, arrows point to clusters of apoptotic cells surrounding the aortic sac. To conclude, our results suggest that in Alk5/Wnt1-Cre mutants a noticeable increase in apoptosis coincides with the abnormal patterning of the PAAs and the aortic sac, and with the failed AP-septation.
T20179	19790-19798	JJ	denotes	Aberrant
T20180	19799-19808	NN	denotes	apoptosis
T20181	19809-19811	IN	denotes	of
T20182	19812-19816	NNS	denotes	NCCs
T20183	19817-19819	IN	denotes	in
T20184	19820-19824	NN	denotes	Alk5
T20186	19824-19825	HYPH	denotes	/
T20187	19825-19829	NN	denotes	Wnt1
T20188	19829-19830	HYPH	denotes	-
T20185	19830-19833	NN	denotes	Cre
T20189	19834-19841	NNS	denotes	mutants
T20190	19841-19842	.	denotes	.
T20191	19842-20208	sentence	denotes	TUNEL (A-H) and Cleaved Caspase-3 (I, J) staining at E11.0 demonstrates a notable increase in apoptosis in Alk5/Wnt1-Cre mutants (B, H, J) on the aortic sac level when compared to controls (A, G, I) or Alk2/Wnt1-Cre mutants (C) (frontal sections), while sections on the OFT level do not demonstrate differences between controls (D) and Alk5 (E) or Alk2 (F) mutants.
T20192	19843-19848	NN	denotes	TUNEL
T20194	19849-19850	-LRB-	denotes	(
T20195	19850-19851	NN	denotes	A
T20196	19851-19852	SYM	denotes	-
T20197	19852-19853	NN	denotes	H
T20198	19853-19854	-RRB-	denotes	)
T20199	19855-19858	CC	denotes	and
T20200	19859-19866	VBN	denotes	Cleaved
T20201	19867-19874	NN	denotes	Caspase
T20202	19874-19875	HYPH	denotes	-
T20203	19875-19876	CD	denotes	3
T20204	19877-19878	-LRB-	denotes	(
T20206	19878-19879	NN	denotes	I
T20207	19879-19881	,	denotes	,
T20205	19881-19882	NN	denotes	J
T20208	19882-19883	-RRB-	denotes	)
T20193	19884-19892	NN	denotes	staining
T20210	19893-19895	IN	denotes	at
T20211	19896-19901	NN	denotes	E11.0
T20209	19902-19914	VBZ	denotes	demonstrates
T20212	19915-19916	DT	denotes	a
T20214	19917-19924	JJ	denotes	notable
T20213	19925-19933	NN	denotes	increase
T20215	19934-19936	IN	denotes	in
T20216	19937-19946	NN	denotes	apoptosis
T20217	19947-19949	IN	denotes	in
T20218	19950-19954	NN	denotes	Alk5
T20220	19954-19955	HYPH	denotes	/
T20221	19955-19959	NN	denotes	Wnt1
T20222	19959-19960	HYPH	denotes	-
T20219	19960-19963	NN	denotes	Cre
T20223	19964-19971	NNS	denotes	mutants
T20224	19972-19973	-LRB-	denotes	(
T20226	19973-19974	NN	denotes	B
T20227	19974-19976	,	denotes	,
T20228	19976-19977	NN	denotes	H
T20229	19977-19979	,	denotes	,
T20225	19979-19980	NN	denotes	J
T20230	19980-19981	-RRB-	denotes	)
T20231	19982-19984	IN	denotes	on
T20232	19985-19988	DT	denotes	the
T20234	19989-19995	JJ	denotes	aortic
T20235	19996-19999	NN	denotes	sac
T20233	20000-20005	NN	denotes	level
T20236	20006-20010	WRB	denotes	when
T20237	20011-20019	VBN	denotes	compared
T20238	20020-20022	IN	denotes	to
T20239	20023-20031	NNS	denotes	controls
T20240	20032-20033	-LRB-	denotes	(
T20242	20033-20034	NN	denotes	A
T20243	20034-20036	,	denotes	,
T20244	20036-20037	NN	denotes	G
T20245	20037-20039	,	denotes	,
T20241	20039-20040	NN	denotes	I
T20246	20040-20041	-RRB-	denotes	)
T20247	20042-20044	CC	denotes	or
T20248	20045-20049	NN	denotes	Alk2
T20250	20049-20050	HYPH	denotes	/
T20251	20050-20054	NN	denotes	Wnt1
T20252	20054-20055	HYPH	denotes	-
T20249	20055-20058	NN	denotes	Cre
T20253	20059-20066	NNS	denotes	mutants
T20254	20067-20068	-LRB-	denotes	(
T20255	20068-20069	NN	denotes	C
T20256	20069-20070	-RRB-	denotes	)
T20257	20071-20072	-LRB-	denotes	(
T20259	20072-20079	JJ	denotes	frontal
T20258	20080-20088	NNS	denotes	sections
T20260	20088-20089	-RRB-	denotes	)
T20261	20089-20091	,	denotes	,
T20262	20091-20096	IN	denotes	while
T20264	20097-20105	NNS	denotes	sections
T20265	20106-20108	IN	denotes	on
T20266	20109-20112	DT	denotes	the
T20268	20113-20116	NN	denotes	OFT
T20267	20117-20122	NN	denotes	level
T20269	20123-20125	VBP	denotes	do
T20270	20126-20129	RB	denotes	not
T20263	20130-20141	VB	denotes	demonstrate
T20271	20142-20153	NNS	denotes	differences
T20272	20154-20161	IN	denotes	between
T20273	20162-20170	NNS	denotes	controls
T20274	20171-20172	-LRB-	denotes	(
T20275	20172-20173	NN	denotes	D
T20276	20173-20174	-RRB-	denotes	)
T20277	20175-20178	CC	denotes	and
T20278	20179-20183	NN	denotes	Alk5
T20280	20184-20185	-LRB-	denotes	(
T20281	20185-20186	NN	denotes	E
T20282	20186-20187	-RRB-	denotes	)
T20283	20188-20190	CC	denotes	or
T20284	20191-20195	NN	denotes	Alk2
T20285	20196-20197	-LRB-	denotes	(
T20286	20197-20198	NN	denotes	F
T20287	20198-20199	-RRB-	denotes	)
T20279	20200-20207	NNS	denotes	mutants
T20288	20207-20208	.	denotes	.
T20289	20208-20314	sentence	denotes	G,H, TUNEL staining of lacZ-stained embryos demonstrates that apoptotic cells are of neural crest origin.
T20290	20209-20210	LS	denotes	G
T20292	20210-20211	,	denotes	,
T20293	20211-20212	LS	denotes	H
T20294	20212-20214	,	denotes	,
T20295	20214-20219	NN	denotes	TUNEL
T20296	20220-20228	NN	denotes	staining
T20297	20229-20231	IN	denotes	of
T20298	20232-20236	NN	denotes	lacZ
T20300	20236-20237	HYPH	denotes	-
T20299	20237-20244	VBN	denotes	stained
T20301	20245-20252	NNS	denotes	embryos
T20291	20253-20265	VBZ	denotes	demonstrates
T20302	20266-20270	IN	denotes	that
T20304	20271-20280	JJ	denotes	apoptotic
T20305	20281-20286	NNS	denotes	cells
T20303	20287-20290	VBP	denotes	are
T20306	20291-20293	IN	denotes	of
T20307	20294-20300	JJ	denotes	neural
T20308	20301-20306	NN	denotes	crest
T20309	20307-20313	NN	denotes	origin
T20310	20313-20314	.	denotes	.
T20311	20314-20337	sentence	denotes	G, control; H, mutant.
T20312	20315-20316	NN	denotes	G
T20313	20316-20318	,	denotes	,
T20314	20318-20325	NN	denotes	control
T20315	20325-20326	:	denotes	;
T20316	20327-20328	NN	denotes	H
T20317	20328-20330	,	denotes	,
T20318	20330-20336	NN	denotes	mutant
T20319	20336-20337	.	denotes	.
T20320	20337-20425	sentence	denotes	AS, aortic sac, arrows point to clusters of apoptotic cells surrounding the aortic sac.
T20321	20338-20340	JJ	denotes	AS
T20323	20340-20342	,	denotes	,
T20324	20342-20348	JJ	denotes	aortic
T20325	20349-20352	NN	denotes	sac
T20326	20352-20354	,	denotes	,
T20327	20354-20360	NNS	denotes	arrows
T20322	20361-20366	VBP	denotes	point
T20328	20367-20369	IN	denotes	to
T20329	20370-20378	NNS	denotes	clusters
T20330	20379-20381	IN	denotes	of
T20331	20382-20391	JJ	denotes	apoptotic
T20332	20392-20397	NNS	denotes	cells
T20333	20398-20409	VBG	denotes	surrounding
T20334	20410-20413	DT	denotes	the
T20336	20414-20420	JJ	denotes	aortic
T20335	20421-20424	NN	denotes	sac
T20337	20424-20425	.	denotes	.
T10130	20426-20428	TO	denotes	To
T10131	20429-20437	VB	denotes	conclude
T10133	20437-20439	,	denotes	,
T10134	20439-20442	PRP$	denotes	our
T10135	20443-20450	NNS	denotes	results
T10132	20451-20458	VBP	denotes	suggest
T10136	20459-20463	IN	denotes	that
T10138	20464-20466	IN	denotes	in
T10139	20467-20471	NN	denotes	Alk5
T10141	20471-20472	HYPH	denotes	/
T10142	20472-20476	NN	denotes	Wnt1
T10143	20476-20477	HYPH	denotes	-
T10140	20477-20480	NN	denotes	Cre
T10144	20481-20488	NNS	denotes	mutants
T10145	20489-20490	DT	denotes	a
T10147	20491-20501	JJ	denotes	noticeable
T10146	20502-20510	NN	denotes	increase
T10148	20511-20513	IN	denotes	in
T10149	20514-20523	NN	denotes	apoptosis
T10137	20524-20533	VBZ	denotes	coincides
T10150	20534-20538	IN	denotes	with
T10151	20539-20542	DT	denotes	the
T10153	20543-20551	JJ	denotes	abnormal
T10152	20552-20562	NN	denotes	patterning
T10154	20563-20565	IN	denotes	of
T10155	20566-20569	DT	denotes	the
T10156	20570-20574	NNS	denotes	PAAs
T10157	20575-20578	CC	denotes	and
T10158	20579-20582	DT	denotes	the
T10160	20583-20589	JJ	denotes	aortic
T10159	20590-20593	NN	denotes	sac
T10161	20593-20595	,	denotes	,
T10162	20595-20598	CC	denotes	and
T10163	20599-20603	IN	denotes	with
T10164	20604-20607	DT	denotes	the
T10166	20608-20614	VBN	denotes	failed
T10167	20615-20617	NN	denotes	AP
T10168	20617-20618	HYPH	denotes	-
T10165	20618-20627	NN	denotes	septation
T10169	20627-20628	.	denotes	.
T10170	20628-20824	sentence	denotes	These data support a specific role for ALK5 signaling, either directly or indirectly, in CNCC survival, since a similar apoptosis of NC-derived cells is not seen in Tgfbr2/Wnt1-Cre mutants [8,9].
T10171	20629-20634	DT	denotes	These
T10172	20635-20639	NNS	denotes	data
T10173	20640-20647	VBP	denotes	support
T10174	20648-20649	DT	denotes	a
T10176	20650-20658	JJ	denotes	specific
T10175	20659-20663	NN	denotes	role
T10177	20664-20667	IN	denotes	for
T10178	20668-20672	NN	denotes	ALK5
T10179	20673-20682	NN	denotes	signaling
T10180	20682-20684	,	denotes	,
T10181	20684-20690	CC	denotes	either
T10182	20691-20699	RB	denotes	directly
T10183	20700-20702	CC	denotes	or
T10184	20703-20713	RB	denotes	indirectly
T10185	20713-20715	,	denotes	,
T10186	20715-20717	IN	denotes	in
T10187	20718-20722	NN	denotes	CNCC
T10188	20723-20731	NN	denotes	survival
T10189	20731-20733	,	denotes	,
T10190	20733-20738	IN	denotes	since
T10192	20739-20740	DT	denotes	a
T10194	20741-20748	JJ	denotes	similar
T10193	20749-20758	NN	denotes	apoptosis
T10195	20759-20761	IN	denotes	of
T10196	20762-20764	NN	denotes	NC
T10198	20764-20765	HYPH	denotes	-
T10197	20765-20772	VBN	denotes	derived
T10199	20773-20778	NNS	denotes	cells
T10200	20779-20781	VBZ	denotes	is
T10201	20782-20785	RB	denotes	not
T10191	20786-20790	VBN	denotes	seen
T10202	20791-20793	IN	denotes	in
T10203	20794-20800	NN	denotes	Tgfbr2
T10205	20800-20801	HYPH	denotes	/
T10206	20801-20805	NN	denotes	Wnt1
T10207	20805-20806	HYPH	denotes	-
T10204	20806-20809	NN	denotes	Cre
T10208	20810-20817	NNS	denotes	mutants
T10209	20818-20819	-LRB-	denotes	[
T10211	20819-20820	CD	denotes	8
T10212	20820-20821	,	denotes	,
T10210	20821-20822	CD	denotes	9
T10213	20822-20823	-RRB-	denotes	]
T10214	20823-20824	.	denotes	.
T10806	20826-20836	JJ	denotes	Pharyngeal
T10807	20837-20843	NNS	denotes	organs
T10808	20844-20848	VBP	denotes	fail
T10809	20849-20851	TO	denotes	to
T10810	20852-20859	VB	denotes	migrate
T10811	20860-20862	IN	denotes	in
T10812	20863-20867	NN	denotes	Alk5
T10814	20867-20868	HYPH	denotes	/
T10815	20868-20872	NN	denotes	Wnt1
T10816	20872-20873	HYPH	denotes	-
T10813	20873-20876	NN	denotes	Cre
T10817	20877-20884	NNS	denotes	mutants
T10818	20884-21058	sentence	denotes	In addition to the cardiac OFT, development of pharyngeal organs, i.e., the parathyroid glands and the thymus was also abnormal in Alk5/Wnt1-Cre mutants (see Figs. 1 and 8).
T10819	20885-20887	IN	denotes	In
T10821	20888-20896	NN	denotes	addition
T10822	20897-20899	IN	denotes	to
T10823	20900-20903	DT	denotes	the
T10825	20904-20911	JJ	denotes	cardiac
T10824	20912-20915	NN	denotes	OFT
T10826	20915-20917	,	denotes	,
T10827	20917-20928	NN	denotes	development
T10828	20929-20931	IN	denotes	of
T10829	20932-20942	JJ	denotes	pharyngeal
T10830	20943-20949	NNS	denotes	organs
T10831	20949-20951	,	denotes	,
T10832	20951-20955	FW	denotes	i.e.
T10834	20955-20957	,	denotes	,
T10835	20957-20960	DT	denotes	the
T10836	20961-20972	JJ	denotes	parathyroid
T10833	20973-20979	NNS	denotes	glands
T10837	20980-20983	CC	denotes	and
T10838	20984-20987	DT	denotes	the
T10839	20988-20994	NN	denotes	thymus
T10820	20995-20998	VBD	denotes	was
T10840	20999-21003	RB	denotes	also
T10841	21004-21012	JJ	denotes	abnormal
T10842	21013-21015	IN	denotes	in
T10843	21016-21020	NN	denotes	Alk5
T10845	21020-21021	HYPH	denotes	/
T10846	21021-21025	NN	denotes	Wnt1
T10847	21025-21026	HYPH	denotes	-
T10844	21026-21029	NN	denotes	Cre
T10848	21030-21037	NNS	denotes	mutants
T10849	21038-21039	-LRB-	denotes	(
T10850	21039-21042	VB	denotes	see
T10851	21043-21048	NNS	denotes	Figs.
T10852	21049-21050	CD	denotes	1
T10853	21051-21054	CC	denotes	and
T10854	21055-21056	CD	denotes	8
T10855	21056-21057	-RRB-	denotes	)
T10856	21057-21058	.	denotes	.
T10857	21058-21240	sentence	denotes	Normally the thymus develops from the third pharyngeal pouch endoderm and migrates caudally to its final location in the superior mediastinum as seen in controls at E14 (Fig. 8A,B).
T10858	21059-21067	RB	denotes	Normally
T10860	21068-21071	DT	denotes	the
T10861	21072-21078	NN	denotes	thymus
T10859	21079-21087	VBZ	denotes	develops
T10862	21088-21092	IN	denotes	from
T10863	21093-21096	DT	denotes	the
T10865	21097-21102	JJ	denotes	third
T10866	21103-21113	JJ	denotes	pharyngeal
T10867	21114-21119	NN	denotes	pouch
T10864	21120-21128	NN	denotes	endoderm
T10868	21129-21132	CC	denotes	and
T10869	21133-21141	VBZ	denotes	migrates
T10870	21142-21150	RB	denotes	caudally
T10871	21151-21153	IN	denotes	to
T10872	21154-21157	PRP$	denotes	its
T10874	21158-21163	JJ	denotes	final
T10873	21164-21172	NN	denotes	location
T10875	21173-21175	IN	denotes	in
T10876	21176-21179	DT	denotes	the
T10878	21180-21188	JJ	denotes	superior
T10877	21189-21200	NN	denotes	mediastinum
T10879	21201-21203	IN	denotes	as
T10880	21204-21208	VBN	denotes	seen
T10881	21209-21211	IN	denotes	in
T10882	21212-21220	NNS	denotes	controls
T10883	21221-21223	IN	denotes	at
T10884	21224-21227	NN	denotes	E14
T10885	21228-21229	-LRB-	denotes	(
T10887	21229-21233	NN	denotes	Fig.
T10888	21234-21236	NN	denotes	8A
T10889	21236-21237	,	denotes	,
T10886	21237-21238	NN	denotes	B
T10890	21238-21239	-RRB-	denotes	)
T10891	21239-21240	.	denotes	.
T10892	21240-21457	sentence	denotes	In contrast, the thymic primordia of the Alk5 mutant littermates failed to descend caudally, and were located bilaterally in the neck region, where they were surrounded by neural crest-derived mesenchyme (Fig. 8D,E).
T10893	21241-21243	IN	denotes	In
T10895	21244-21252	NN	denotes	contrast
T10896	21252-21254	,	denotes	,
T10897	21254-21257	DT	denotes	the
T10899	21258-21264	JJ	denotes	thymic
T10898	21265-21274	NNS	denotes	primordia
T10900	21275-21277	IN	denotes	of
T10901	21278-21281	DT	denotes	the
T10903	21282-21286	NN	denotes	Alk5
T10904	21287-21293	NN	denotes	mutant
T10902	21294-21305	NNS	denotes	littermates
T10894	21306-21312	VBD	denotes	failed
T10905	21313-21315	TO	denotes	to
T10906	21316-21323	VB	denotes	descend
T10907	21324-21332	RB	denotes	caudally
T10908	21332-21334	,	denotes	,
T10909	21334-21337	CC	denotes	and
T10910	21338-21342	VBD	denotes	were
T10911	21343-21350	VBN	denotes	located
T10912	21351-21362	RB	denotes	bilaterally
T10913	21363-21365	IN	denotes	in
T10914	21366-21369	DT	denotes	the
T10916	21370-21374	NN	denotes	neck
T10915	21375-21381	NN	denotes	region
T10917	21381-21383	,	denotes	,
T10918	21383-21388	WRB	denotes	where
T10920	21389-21393	PRP	denotes	they
T10921	21394-21398	VBD	denotes	were
T10919	21399-21409	VBN	denotes	surrounded
T10922	21410-21412	IN	denotes	by
T10923	21413-21419	JJ	denotes	neural
T10924	21420-21425	NN	denotes	crest
T10926	21425-21426	HYPH	denotes	-
T10925	21426-21433	VBN	denotes	derived
T10927	21434-21444	NN	denotes	mesenchyme
T10928	21445-21446	-LRB-	denotes	(
T10930	21446-21450	NN	denotes	Fig.
T10931	21451-21453	NN	denotes	8D
T10932	21453-21454	,	denotes	,
T10929	21454-21455	NN	denotes	E
T10933	21455-21456	-RRB-	denotes	)
T10934	21456-21457	.	denotes	.
T10935	21457-21603	sentence	denotes	The fate determination assay demonstrated that the thymic primordia were equally populated by NCCs both in controls and Alk5 mutants (Fig. 8B,E).
T10936	21458-21461	DT	denotes	The
T10938	21462-21466	NN	denotes	fate
T10939	21467-21480	NN	denotes	determination
T10937	21481-21486	NN	denotes	assay
T10940	21487-21499	VBD	denotes	demonstrated
T10941	21500-21504	IN	denotes	that
T10943	21505-21508	DT	denotes	the
T10945	21509-21515	JJ	denotes	thymic
T10944	21516-21525	NNS	denotes	primordia
T10946	21526-21530	VBD	denotes	were
T10947	21531-21538	RB	denotes	equally
T10942	21539-21548	VBN	denotes	populated
T10948	21549-21551	IN	denotes	by
T10949	21552-21556	NNS	denotes	NCCs
T10950	21557-21561	CC	denotes	both
T10951	21562-21564	IN	denotes	in
T10952	21565-21573	NNS	denotes	controls
T10953	21574-21577	CC	denotes	and
T10954	21578-21582	NN	denotes	Alk5
T10955	21583-21590	NNS	denotes	mutants
T10956	21591-21592	-LRB-	denotes	(
T10958	21592-21596	NN	denotes	Fig.
T10959	21597-21599	NN	denotes	8B
T10960	21599-21600	,	denotes	,
T10957	21600-21601	NN	denotes	E
T10961	21601-21602	-RRB-	denotes	)
T10962	21602-21603	.	denotes	.
T10963	21603-21688	sentence	denotes	Likewise the parathyroid glands failed to migrate normally in Alk5/Wnt1-Cre mutants.
T10964	21604-21612	RB	denotes	Likewise
T10966	21613-21616	DT	denotes	the
T10968	21617-21628	JJ	denotes	parathyroid
T10967	21629-21635	NNS	denotes	glands
T10965	21636-21642	VBD	denotes	failed
T10969	21643-21645	TO	denotes	to
T10970	21646-21653	VB	denotes	migrate
T10971	21654-21662	RB	denotes	normally
T10972	21663-21665	IN	denotes	in
T10973	21666-21670	NN	denotes	Alk5
T10975	21670-21671	HYPH	denotes	/
T10976	21671-21675	NN	denotes	Wnt1
T10977	21675-21676	HYPH	denotes	-
T10974	21676-21679	NN	denotes	Cre
T10978	21680-21687	NNS	denotes	mutants
T10979	21687-21688	.	denotes	.
T10980	21688-21856	sentence	denotes	During normal development, the parathyroids first migrate in association with the thymic primordia, until they reach the thyroids in the neck region as seen in Fig 8C.
T10981	21689-21695	IN	denotes	During
T10983	21696-21702	JJ	denotes	normal
T10984	21703-21714	NN	denotes	development
T10985	21714-21716	,	denotes	,
T10986	21716-21719	DT	denotes	the
T10987	21720-21732	NNS	denotes	parathyroids
T10988	21733-21738	RB	denotes	first
T10982	21739-21746	VBP	denotes	migrate
T10989	21747-21749	IN	denotes	in
T10990	21750-21761	NN	denotes	association
T10991	21762-21766	IN	denotes	with
T10992	21767-21770	DT	denotes	the
T10994	21771-21777	JJ	denotes	thymic
T10993	21778-21787	NNS	denotes	primordia
T10995	21787-21789	,	denotes	,
T10996	21789-21794	IN	denotes	until
T10998	21795-21799	PRP	denotes	they
T10997	21800-21805	VBP	denotes	reach
T10999	21806-21809	DT	denotes	the
T11000	21810-21818	NNS	denotes	thyroids
T11001	21819-21821	IN	denotes	in
T11002	21822-21825	DT	denotes	the
T11004	21826-21830	NN	denotes	neck
T11003	21831-21837	NN	denotes	region
T11005	21838-21840	IN	denotes	as
T11006	21841-21845	VBN	denotes	seen
T11007	21846-21848	IN	denotes	in
T11008	21849-21852	NN	denotes	Fig
T11009	21853-21855	NN	denotes	8C
T11010	21855-21856	.	denotes	.
T11011	21856-22100	sentence	denotes	In Alk5/Wnt1-Cre mutants, the parathyroids remained associated with the thymic primordia, and despite this abnormal rostral location, expression of parathyroid hormone was indistinguishable between Alk5 mutants and controls at E14 (Fig. 8C,F).
T11012	21857-21859	IN	denotes	In
T11014	21860-21864	NN	denotes	Alk5
T11016	21864-21865	HYPH	denotes	/
T11017	21865-21869	NN	denotes	Wnt1
T11018	21869-21870	HYPH	denotes	-
T11015	21870-21873	NN	denotes	Cre
T11019	21874-21881	NNS	denotes	mutants
T11020	21881-21883	,	denotes	,
T11021	21883-21886	DT	denotes	the
T11022	21887-21899	NNS	denotes	parathyroids
T11013	21900-21908	VBD	denotes	remained
T11023	21909-21919	JJ	denotes	associated
T11024	21920-21924	IN	denotes	with
T11025	21925-21928	DT	denotes	the
T11027	21929-21935	JJ	denotes	thymic
T11026	21936-21945	NNS	denotes	primordia
T11028	21945-21947	,	denotes	,
T11029	21947-21950	CC	denotes	and
T11030	21951-21958	IN	denotes	despite
T11032	21959-21963	DT	denotes	this
T11034	21964-21972	JJ	denotes	abnormal
T11035	21973-21980	JJ	denotes	rostral
T11033	21981-21989	NN	denotes	location
T11036	21989-21991	,	denotes	,
T11037	21991-22001	NN	denotes	expression
T11038	22002-22004	IN	denotes	of
T11039	22005-22016	NN	denotes	parathyroid
T11040	22017-22024	NN	denotes	hormone
T11031	22025-22028	VBD	denotes	was
T11041	22029-22046	JJ	denotes	indistinguishable
T11042	22047-22054	IN	denotes	between
T11043	22055-22059	NN	denotes	Alk5
T11044	22060-22067	NNS	denotes	mutants
T11045	22068-22071	CC	denotes	and
T11046	22072-22080	NNS	denotes	controls
T11047	22081-22083	IN	denotes	at
T11048	22084-22087	NN	denotes	E14
T11049	22088-22089	-LRB-	denotes	(
T11051	22089-22093	NN	denotes	Fig.
T11052	22094-22096	NN	denotes	8C
T11053	22096-22097	,	denotes	,
T11050	22097-22098	NN	denotes	F
T11054	22098-22099	-RRB-	denotes	)
T11055	22099-22100	.	denotes	.
T11056	22100-22233	sentence	denotes	To conclude, the observed pharyngeal organ phenotypes were also in striking contrast to those seen in Tgfbr2/Wnt1-Cre mutants [8,9].
T11057	22101-22103	TO	denotes	To
T11058	22104-22112	VB	denotes	conclude
T11060	22112-22114	,	denotes	,
T11061	22114-22117	DT	denotes	the
T11063	22118-22126	VBN	denotes	observed
T11064	22127-22137	JJ	denotes	pharyngeal
T11065	22138-22143	NN	denotes	organ
T11062	22144-22154	NNS	denotes	phenotypes
T11059	22155-22159	VBD	denotes	were
T11066	22160-22164	RB	denotes	also
T11067	22165-22167	IN	denotes	in
T11068	22168-22176	NN	denotes	striking
T11069	22177-22185	NN	denotes	contrast
T11070	22186-22188	IN	denotes	to
T11071	22189-22194	DT	denotes	those
T11072	22195-22199	VBN	denotes	seen
T11073	22200-22202	IN	denotes	in
T11074	22203-22209	NN	denotes	Tgfbr2
T11076	22209-22210	HYPH	denotes	/
T11077	22210-22214	NN	denotes	Wnt1
T11078	22214-22215	HYPH	denotes	-
T11075	22215-22218	NN	denotes	Cre
T11079	22219-22226	NNS	denotes	mutants
T11080	22227-22228	-LRB-	denotes	[
T11082	22228-22229	CD	denotes	8
T11083	22229-22230	,	denotes	,
T11081	22230-22231	CD	denotes	9
T11084	22231-22232	-RRB-	denotes	]
T11085	22232-22233	.	denotes	.
T20789	22244-22254	JJ	denotes	Pharyngeal
T20790	22255-22261	NNS	denotes	organs
T20791	22262-22266	VBP	denotes	fail
T20792	22267-22269	TO	denotes	to
T20793	22270-22277	VB	denotes	migrate
T20794	22278-22280	IN	denotes	in
T20795	22281-22285	NN	denotes	Alk5
T20797	22285-22286	HYPH	denotes	/
T20798	22286-22290	NN	denotes	Wnt1
T20799	22290-22291	HYPH	denotes	-
T20796	22291-22294	NN	denotes	Cre
T20800	22295-22302	NNS	denotes	mutants
T20801	22302-22303	.	denotes	.
T20802	22303-22435	sentence	denotes	At E14.0, the thymus was not detectable in the superior mediastinum (asterisks) in Alk5 mutants (D), when compared to controls (A).
T20803	22304-22306	IN	denotes	At
T20805	22307-22312	NN	denotes	E14.0
T20806	22312-22314	,	denotes	,
T20807	22314-22317	DT	denotes	the
T20808	22318-22324	NN	denotes	thymus
T20804	22325-22328	VBD	denotes	was
T20809	22329-22332	RB	denotes	not
T20810	22333-22343	JJ	denotes	detectable
T20811	22344-22346	IN	denotes	in
T20812	22347-22350	DT	denotes	the
T20814	22351-22359	JJ	denotes	superior
T20813	22360-22371	NN	denotes	mediastinum
T20815	22372-22373	-LRB-	denotes	(
T20816	22373-22382	NNS	denotes	asterisks
T20817	22382-22383	-RRB-	denotes	)
T20818	22384-22386	IN	denotes	in
T20819	22387-22391	NN	denotes	Alk5
T20820	22392-22399	NNS	denotes	mutants
T20821	22400-22401	-LRB-	denotes	(
T20822	22401-22402	NN	denotes	D
T20823	22402-22403	-RRB-	denotes	)
T20824	22403-22405	,	denotes	,
T20825	22405-22409	WRB	denotes	when
T20826	22410-22418	VBN	denotes	compared
T20827	22419-22421	IN	denotes	to
T20828	22422-22430	NNS	denotes	controls
T20829	22431-22432	-LRB-	denotes	(
T20830	22432-22433	NN	denotes	A
T20831	22433-22434	-RRB-	denotes	)
T20832	22434-22435	.	denotes	.
T20833	22435-22648	sentence	denotes	Serial sectioning revealed that the thymic primordia had failed to descend and were seen bilaterally in the upper pharyngeal region (E, F) surrounded by neural crest derived mesenchyme (blue staining cells in E).
T20834	22436-22442	JJ	denotes	Serial
T20835	22443-22453	NN	denotes	sectioning
T20836	22454-22462	VBD	denotes	revealed
T20837	22463-22467	IN	denotes	that
T20839	22468-22471	DT	denotes	the
T20841	22472-22478	JJ	denotes	thymic
T20840	22479-22488	NNS	denotes	primordia
T20842	22489-22492	VBD	denotes	had
T20838	22493-22499	VBN	denotes	failed
T20843	22500-22502	TO	denotes	to
T20844	22503-22510	VB	denotes	descend
T20845	22511-22514	CC	denotes	and
T20846	22515-22519	VBD	denotes	were
T20847	22520-22524	VBN	denotes	seen
T20848	22525-22536	RB	denotes	bilaterally
T20849	22537-22539	IN	denotes	in
T20850	22540-22543	DT	denotes	the
T20852	22544-22549	JJ	denotes	upper
T20853	22550-22560	JJ	denotes	pharyngeal
T20851	22561-22567	NN	denotes	region
T20854	22568-22569	-LRB-	denotes	(
T20856	22569-22570	NN	denotes	E
T20857	22570-22572	,	denotes	,
T20855	22572-22573	NN	denotes	F
T20858	22573-22574	-RRB-	denotes	)
T20859	22575-22585	VBN	denotes	surrounded
T20860	22586-22588	IN	denotes	by
T20861	22589-22595	JJ	denotes	neural
T20862	22596-22601	NN	denotes	crest
T20863	22602-22609	VBN	denotes	derived
T20864	22610-22620	NN	denotes	mesenchyme
T20865	22621-22622	-LRB-	denotes	(
T20867	22622-22626	JJ	denotes	blue
T20868	22627-22635	NN	denotes	staining
T20866	22636-22641	NNS	denotes	cells
T20869	22642-22644	IN	denotes	in
T20870	22645-22646	NN	denotes	E
T20871	22646-22647	-RRB-	denotes	)
T20872	22647-22648	.	denotes	.
T20873	22648-22850	sentence	denotes	In controls, the parathyroid glands were properly associated with the thyroid glands (arrows in C), while in Alk5 mutants the parathyroid glands were associated with the thymic primordia (arrows in F).
T20874	22649-22651	IN	denotes	In
T20876	22652-22660	NNS	denotes	controls
T20877	22660-22662	,	denotes	,
T20878	22662-22665	DT	denotes	the
T20880	22666-22677	JJ	denotes	parathyroid
T20879	22678-22684	NNS	denotes	glands
T20881	22685-22689	VBD	denotes	were
T20882	22690-22698	RB	denotes	properly
T20875	22699-22709	VBN	denotes	associated
T20883	22710-22714	IN	denotes	with
T20884	22715-22718	DT	denotes	the
T20886	22719-22726	JJ	denotes	thyroid
T20885	22727-22733	NNS	denotes	glands
T20887	22734-22735	-LRB-	denotes	(
T20888	22735-22741	NNS	denotes	arrows
T20889	22742-22744	IN	denotes	in
T20890	22745-22746	NN	denotes	C
T20891	22746-22747	-RRB-	denotes	)
T20892	22747-22749	,	denotes	,
T20893	22749-22754	IN	denotes	while
T20895	22755-22757	IN	denotes	in
T20896	22758-22762	NN	denotes	Alk5
T20897	22763-22770	NNS	denotes	mutants
T20898	22771-22774	DT	denotes	the
T20900	22775-22786	JJ	denotes	parathyroid
T20899	22787-22793	NNS	denotes	glands
T20901	22794-22798	VBD	denotes	were
T20894	22799-22809	VBN	denotes	associated
T20902	22810-22814	IN	denotes	with
T20903	22815-22818	DT	denotes	the
T20905	22819-22825	JJ	denotes	thymic
T20904	22826-22835	NNS	denotes	primordia
T20906	22836-22837	-LRB-	denotes	(
T20907	22837-22843	NNS	denotes	arrows
T20908	22844-22846	IN	denotes	in
T20909	22847-22848	NN	denotes	F
T20910	22848-22849	-RRB-	denotes	)
T20911	22849-22850	.	denotes	.
T20912	22850-22970	sentence	denotes	However, both controls and mutants expressed parathyroid hormone (PTH) at comparable levels (blue staining in C and F).
T20913	22851-22858	RB	denotes	However
T20915	22858-22860	,	denotes	,
T20916	22860-22864	DT	denotes	both
T20917	22865-22873	NNS	denotes	controls
T20918	22874-22877	CC	denotes	and
T20919	22878-22885	NNS	denotes	mutants
T20914	22886-22895	VBD	denotes	expressed
T20920	22896-22907	NN	denotes	parathyroid
T20921	22908-22915	NN	denotes	hormone
T20922	22916-22917	-LRB-	denotes	(
T20923	22917-22920	NN	denotes	PTH
T20924	22920-22921	-RRB-	denotes	)
T20925	22922-22924	IN	denotes	at
T20926	22925-22935	JJ	denotes	comparable
T20927	22936-22942	NNS	denotes	levels
T20928	22943-22944	-LRB-	denotes	(
T20930	22944-22948	JJ	denotes	blue
T20929	22949-22957	NN	denotes	staining
T20931	22958-22960	IN	denotes	in
T20932	22961-22962	NN	denotes	C
T20933	22963-22966	CC	denotes	and
T20934	22967-22968	NN	denotes	F
T20935	22968-22969	-RRB-	denotes	)
T20936	22969-22970	.	denotes	.
T20937	22970-23156	sentence	denotes	A and D, hematoxyllin and eosin staining; B and E, R26 R lineage tracing assay – counterstaining with eosin; C and F, section in situ hybridization for PTH – counterstaining with eosin.
T20938	22971-22972	LS	denotes	A
T20940	22973-22976	CC	denotes	and
T20941	22977-22978	LS	denotes	D
T20942	22978-22980	,	denotes	,
T20943	22980-22992	NN	denotes	hematoxyllin
T20944	22993-22996	CC	denotes	and
T20945	22997-23002	NN	denotes	eosin
T20939	23003-23011	NN	denotes	staining
T20946	23011-23012	:	denotes	;
T20947	23013-23014	LS	denotes	B
T20949	23015-23018	CC	denotes	and
T20950	23019-23020	LS	denotes	E
T20951	23020-23022	,	denotes	,
T20952	23022-23025	NN	denotes	R26
T20948	23026-23027	NN	denotes	R
T20953	23028-23035	NN	denotes	lineage
T20955	23036-23043	NN	denotes	tracing
T20954	23044-23049	NN	denotes	assay
T20956	23050-23051	HYPH	denotes	–
T20957	23052-23067	VBG	denotes	counterstaining
T20958	23068-23072	IN	denotes	with
T20959	23073-23078	NN	denotes	eosin
T20960	23078-23079	:	denotes	;
T20961	23080-23081	LS	denotes	C
T20963	23082-23085	CC	denotes	and
T20964	23086-23087	LS	denotes	F
T20965	23087-23089	,	denotes	,
T20966	23089-23096	NN	denotes	section
T20967	23097-23099	FW	denotes	in
T20968	23100-23104	FW	denotes	situ
T20962	23105-23118	NN	denotes	hybridization
T20969	23119-23122	IN	denotes	for
T20970	23123-23126	NN	denotes	PTH
T20971	23127-23128	HYPH	denotes	–
T20972	23129-23144	VBG	denotes	counterstaining
T20973	23145-23149	IN	denotes	with
T20974	23150-23155	NN	denotes	eosin
T20975	23155-23156	.	denotes	.
T20976	23156-23279	sentence	denotes	T, thymus; Th, thyroid; asterisks in D depict the absence of the thymic primordia; asterisks in E and F depict the tongue.
T20977	23157-23158	NN	denotes	T
T20979	23158-23160	,	denotes	,
T20980	23160-23166	NN	denotes	thymus
T20981	23166-23167	:	denotes	;
T20982	23168-23170	NN	denotes	Th
T20983	23170-23172	,	denotes	,
T20984	23172-23179	NN	denotes	thyroid
T20985	23179-23180	:	denotes	;
T20986	23181-23190	NNS	denotes	asterisks
T20988	23191-23193	IN	denotes	in
T20989	23194-23195	NN	denotes	D
T20987	23196-23202	VBP	denotes	depict
T20990	23203-23206	DT	denotes	the
T20991	23207-23214	NN	denotes	absence
T20992	23215-23217	IN	denotes	of
T20993	23218-23221	DT	denotes	the
T20995	23222-23228	JJ	denotes	thymic
T20994	23229-23238	NNS	denotes	primordia
T20996	23238-23239	:	denotes	;
T20997	23240-23249	NNS	denotes	asterisks
T20998	23250-23252	IN	denotes	in
T20999	23253-23254	NN	denotes	E
T21000	23255-23258	CC	denotes	and
T21001	23259-23260	NN	denotes	F
T20978	23261-23267	VBP	denotes	depict
T21002	23268-23271	DT	denotes	the
T21003	23272-23278	NN	denotes	tongue
T21004	23278-23279	.	denotes	.
T13939	23292-23298	IN	denotes	During
T13941	23299-23302	DT	denotes	the
T13943	23303-23307	JJ	denotes	last
T13944	23308-23311	JJ	denotes	few
T13942	23312-23317	NNS	denotes	years
T13945	23318-23321	DT	denotes	the
T13947	23322-23326	NN	denotes	Wnt1
T13949	23326-23327	HYPH	denotes	-
T13948	23327-23330	NN	denotes	Cre
T13950	23331-23341	JJ	denotes	transgenic
T13951	23342-23348	NN	denotes	driver
T13946	23349-23353	NN	denotes	line
T13952	23354-23357	VBZ	denotes	has
T13940	23358-23364	VBN	denotes	proven
T13953	23365-23367	TO	denotes	to
T13954	23368-23370	VB	denotes	be
T13955	23371-23372	DT	denotes	a
T13957	23373-23381	JJ	denotes	powerful
T13956	23382-23386	NN	denotes	tool
T13958	23387-23390	IN	denotes	for
T13959	23391-23397	NN	denotes	tissue
T13961	23397-23398	HYPH	denotes	-
T13960	23398-23406	JJ	denotes	specific
T13963	23407-23411	NN	denotes	gene
T13962	23412-23420	NN	denotes	deletion
T13964	23421-23423	IN	denotes	in
T13965	23424-23428	NNS	denotes	NCCs
T13966	23429-23430	-LRB-	denotes	[
T13968	23430-23432	CD	denotes	12
T13969	23432-23433	,	denotes	,
T13970	23433-23435	CD	denotes	13
T13971	23435-23436	,	denotes	,
T13972	23436-23438	CD	denotes	27
T13973	23438-23439	,	denotes	,
T13967	23439-23441	CD	denotes	28
T13974	23441-23442	-RRB-	denotes	]
T13975	23442-23443	.	denotes	.
T13976	23443-23632	sentence	denotes	Using this approach, several studies have independently shown that the NC-specific deletion of the Tgfbr2 gene leads to a distinct set of calvarial, facial and cardiac defects [8,9,20,29].
T13977	23444-23449	VBG	denotes	Using
T13979	23450-23454	DT	denotes	this
T13980	23455-23463	NN	denotes	approach
T13981	23463-23465	,	denotes	,
T13982	23465-23472	JJ	denotes	several
T13983	23473-23480	NNS	denotes	studies
T13984	23481-23485	VBP	denotes	have
T13985	23486-23499	RB	denotes	independently
T13978	23500-23505	VBN	denotes	shown
T13986	23506-23510	IN	denotes	that
T13988	23511-23514	DT	denotes	the
T13990	23515-23517	NN	denotes	NC
T13992	23517-23518	HYPH	denotes	-
T13991	23518-23526	JJ	denotes	specific
T13989	23527-23535	NN	denotes	deletion
T13993	23536-23538	IN	denotes	of
T13994	23539-23542	DT	denotes	the
T13996	23543-23549	NN	denotes	Tgfbr2
T13995	23550-23554	NN	denotes	gene
T13987	23555-23560	VBZ	denotes	leads
T13997	23561-23563	IN	denotes	to
T13998	23564-23565	DT	denotes	a
T14000	23566-23574	JJ	denotes	distinct
T13999	23575-23578	NN	denotes	set
T14001	23579-23581	IN	denotes	of
T14002	23582-23591	JJ	denotes	calvarial
T14004	23591-23593	,	denotes	,
T14005	23593-23599	JJ	denotes	facial
T14006	23600-23603	CC	denotes	and
T14007	23604-23611	JJ	denotes	cardiac
T14003	23612-23619	NNS	denotes	defects
T14008	23620-23621	-LRB-	denotes	[
T14010	23621-23622	CD	denotes	8
T14011	23622-23623	,	denotes	,
T14012	23623-23624	CD	denotes	9
T14013	23624-23625	,	denotes	,
T14014	23625-23627	CD	denotes	20
T14015	23627-23628	,	denotes	,
T14009	23628-23630	CD	denotes	29
T14016	23630-23631	-RRB-	denotes	]
T14017	23631-23632	.	denotes	.
T14018	23632-23919	sentence	denotes	Interestingly, these defects appear quite different both in the craniofacial and pharyngeal regions, including the heart, when compared to the corresponding mutants of Alk5, which encodes the TGF-β type I receptor, a prototypical binding partner of TGF-βRII [19] and the present study).
T14019	23633-23646	RB	denotes	Interestingly
T14021	23646-23648	,	denotes	,
T14022	23648-23653	DT	denotes	these
T14023	23654-23661	NNS	denotes	defects
T14020	23662-23668	VBP	denotes	appear
T14024	23669-23674	RB	denotes	quite
T14025	23675-23684	JJ	denotes	different
T14026	23685-23689	CC	denotes	both
T14027	23690-23692	IN	denotes	in
T14028	23693-23696	DT	denotes	the
T14030	23697-23709	JJ	denotes	craniofacial
T14031	23710-23713	CC	denotes	and
T14032	23714-23724	JJ	denotes	pharyngeal
T14029	23725-23732	NNS	denotes	regions
T14033	23732-23734	,	denotes	,
T14034	23734-23743	VBG	denotes	including
T14035	23744-23747	DT	denotes	the
T14036	23748-23753	NN	denotes	heart
T14037	23753-23755	,	denotes	,
T14038	23755-23759	WRB	denotes	when
T14039	23760-23768	VBN	denotes	compared
T14040	23769-23771	IN	denotes	to
T14041	23772-23775	DT	denotes	the
T14043	23776-23789	VBG	denotes	corresponding
T14042	23790-23797	NNS	denotes	mutants
T14044	23798-23800	IN	denotes	of
T14045	23801-23805	NN	denotes	Alk5
T14046	23805-23807	,	denotes	,
T14047	23807-23812	WDT	denotes	which
T14048	23813-23820	VBZ	denotes	encodes
T14049	23821-23824	DT	denotes	the
T14051	23825-23828	NN	denotes	TGF
T14053	23828-23829	HYPH	denotes	-
T14052	23829-23830	NN	denotes	β
T14054	23831-23835	NN	denotes	type
T14055	23836-23837	CD	denotes	I
T14050	23838-23846	NN	denotes	receptor
T14056	23846-23848	,	denotes	,
T14057	23848-23849	DT	denotes	a
T14059	23850-23862	JJ	denotes	prototypical
T14060	23863-23870	NN	denotes	binding
T14058	23871-23878	NN	denotes	partner
T14061	23879-23881	IN	denotes	of
T14062	23882-23885	NN	denotes	TGF
T14064	23885-23886	HYPH	denotes	-
T14063	23886-23890	NN	denotes	βRII
T14065	23891-23892	-LRB-	denotes	[
T14066	23892-23894	CD	denotes	19
T14067	23894-23895	-RRB-	denotes	]
T14068	23896-23899	CC	denotes	and
T14069	23900-23903	DT	denotes	the
T14071	23904-23911	JJ	denotes	present
T14070	23912-23917	NN	denotes	study
T14072	23917-23918	-RRB-	denotes	)
T14073	23918-23919	.	denotes	.
T14074	23919-24212	sentence	denotes	While Tgfbr2/Wnt1-Cre mutants as well as mice deficient in Tgf-β2 display the PTA type A4 (truncus arteriosus with interrupted aortic arch [30]), Alk5/Wnt1-Cre mutants reported here demonstrate earlier patterning defects of the PAAs, which is particularly obvious in the 3rd pair of the PAAs.
T14075	23920-23925	IN	denotes	While
T14077	23926-23932	NN	denotes	Tgfbr2
T14079	23932-23933	HYPH	denotes	/
T14080	23933-23937	NN	denotes	Wnt1
T14081	23937-23938	HYPH	denotes	-
T14078	23938-23941	NN	denotes	Cre
T14082	23942-23949	NNS	denotes	mutants
T14083	23950-23952	RB	denotes	as
T14085	23953-23957	RB	denotes	well
T14084	23958-23960	IN	denotes	as
T14086	23961-23965	NNS	denotes	mice
T14087	23966-23975	JJ	denotes	deficient
T14088	23976-23978	IN	denotes	in
T14089	23979-23982	NN	denotes	Tgf
T14091	23982-23983	HYPH	denotes	-
T14090	23983-23985	NN	denotes	β2
T14076	23986-23993	VBP	denotes	display
T14093	23994-23997	DT	denotes	the
T14095	23998-24001	NN	denotes	PTA
T14096	24002-24006	NN	denotes	type
T14094	24007-24009	NN	denotes	A4
T14097	24010-24011	-LRB-	denotes	(
T14098	24011-24018	NN	denotes	truncus
T14099	24019-24029	NN	denotes	arteriosus
T14100	24030-24034	IN	denotes	with
T14101	24035-24046	VBN	denotes	interrupted
T14103	24047-24053	JJ	denotes	aortic
T14102	24054-24058	NN	denotes	arch
T14104	24059-24060	-LRB-	denotes	[
T14105	24060-24062	CD	denotes	30
T14106	24062-24063	-RRB-	denotes	]
T14107	24063-24064	-RRB-	denotes	)
T14108	24064-24066	,	denotes	,
T14109	24066-24070	NN	denotes	Alk5
T14111	24070-24071	HYPH	denotes	/
T14112	24071-24075	NN	denotes	Wnt1
T14113	24075-24076	HYPH	denotes	-
T14110	24076-24079	NN	denotes	Cre
T14114	24080-24087	NNS	denotes	mutants
T14115	24088-24096	VBN	denotes	reported
T14116	24097-24101	RB	denotes	here
T14092	24102-24113	VBP	denotes	demonstrate
T14117	24114-24121	JJR	denotes	earlier
T14119	24122-24132	NN	denotes	patterning
T14118	24133-24140	NNS	denotes	defects
T14120	24141-24143	IN	denotes	of
T14121	24144-24147	DT	denotes	the
T14122	24148-24152	NNS	denotes	PAAs
T14123	24152-24154	,	denotes	,
T14124	24154-24159	WDT	denotes	which
T14125	24160-24162	VBZ	denotes	is
T14126	24163-24175	RB	denotes	particularly
T14127	24176-24183	JJ	denotes	obvious
T14128	24184-24186	IN	denotes	in
T14129	24187-24190	DT	denotes	the
T14131	24191-24194	JJ	denotes	3rd
T14130	24195-24199	NN	denotes	pair
T14132	24200-24202	IN	denotes	of
T14133	24203-24206	DT	denotes	the
T14134	24207-24211	NNS	denotes	PAAs
T14135	24211-24212	.	denotes	.
T14136	24212-24437	sentence	denotes	Moreover, the Alk5/Wnt1-Cre mutants display an abnormal patterning of the aortic sac and defective AP septation leading to PTA, reminiscent of type A2 (= truncus artriosus with no main pulmonary artery segment present [30]).
T14137	24213-24221	RB	denotes	Moreover
T14139	24221-24223	,	denotes	,
T14140	24223-24226	DT	denotes	the
T14142	24227-24231	NN	denotes	Alk5
T14144	24231-24232	HYPH	denotes	/
T14145	24232-24236	NN	denotes	Wnt1
T14146	24236-24237	HYPH	denotes	-
T14143	24237-24240	NN	denotes	Cre
T14141	24241-24248	NNS	denotes	mutants
T14138	24249-24256	VBP	denotes	display
T14147	24257-24259	DT	denotes	an
T14149	24260-24268	JJ	denotes	abnormal
T14148	24269-24279	NN	denotes	patterning
T14150	24280-24282	IN	denotes	of
T14151	24283-24286	DT	denotes	the
T14153	24287-24293	JJ	denotes	aortic
T14152	24294-24297	NN	denotes	sac
T14154	24298-24301	CC	denotes	and
T14155	24302-24311	JJ	denotes	defective
T14157	24312-24314	NN	denotes	AP
T14156	24315-24324	NN	denotes	septation
T14158	24325-24332	VBG	denotes	leading
T14159	24333-24335	IN	denotes	to
T14160	24336-24339	NN	denotes	PTA
T14161	24339-24341	,	denotes	,
T14162	24341-24352	JJ	denotes	reminiscent
T14163	24353-24355	IN	denotes	of
T14164	24356-24360	NN	denotes	type
T14165	24361-24363	NN	denotes	A2
T14166	24364-24365	-LRB-	denotes	(
T14167	24365-24366	SYM	denotes	=
T14169	24367-24374	NN	denotes	truncus
T14168	24375-24384	NN	denotes	artriosus
T14170	24385-24389	IN	denotes	with
T14171	24390-24392	DT	denotes	no
T14173	24393-24397	JJ	denotes	main
T14174	24398-24407	JJ	denotes	pulmonary
T14175	24408-24414	NN	denotes	artery
T14172	24415-24422	NN	denotes	segment
T14176	24423-24430	JJ	denotes	present
T14177	24431-24432	-LRB-	denotes	[
T14178	24432-24434	CD	denotes	30
T14179	24434-24435	-RRB-	denotes	]
T14180	24435-24436	-RRB-	denotes	)
T14181	24436-24437	.	denotes	.
T14182	24437-24681	sentence	denotes	However, our results also demonstrate that significant hypoplasia of the aortic sac leads to a severe shortening of the ascending truncal arch, which masks possible defects in derivatives of the 4th PAAs, i.e., interruption of the aortic arch.
T14183	24438-24445	RB	denotes	However
T14185	24445-24447	,	denotes	,
T14186	24447-24450	PRP$	denotes	our
T14187	24451-24458	NNS	denotes	results
T14188	24459-24463	RB	denotes	also
T14184	24464-24475	VBP	denotes	demonstrate
T14189	24476-24480	IN	denotes	that
T14191	24481-24492	JJ	denotes	significant
T14192	24493-24503	NN	denotes	hypoplasia
T14193	24504-24506	IN	denotes	of
T14194	24507-24510	DT	denotes	the
T14196	24511-24517	JJ	denotes	aortic
T14195	24518-24521	NN	denotes	sac
T14190	24522-24527	VBZ	denotes	leads
T14197	24528-24530	IN	denotes	to
T14198	24531-24532	DT	denotes	a
T14200	24533-24539	JJ	denotes	severe
T14199	24540-24550	NN	denotes	shortening
T14201	24551-24553	IN	denotes	of
T14202	24554-24557	DT	denotes	the
T14204	24558-24567	VBG	denotes	ascending
T14205	24568-24575	JJ	denotes	truncal
T14203	24576-24580	NN	denotes	arch
T14206	24580-24582	,	denotes	,
T14207	24582-24587	WDT	denotes	which
T14208	24588-24593	VBZ	denotes	masks
T14209	24594-24602	JJ	denotes	possible
T14210	24603-24610	NNS	denotes	defects
T14211	24611-24613	IN	denotes	in
T14212	24614-24625	NNS	denotes	derivatives
T14213	24626-24628	IN	denotes	of
T14214	24629-24632	DT	denotes	the
T14216	24633-24636	JJ	denotes	4th
T14215	24637-24641	NNS	denotes	PAAs
T14217	24641-24643	,	denotes	,
T14218	24643-24647	FW	denotes	i.e.
T14220	24647-24649	,	denotes	,
T14219	24649-24661	NN	denotes	interruption
T14221	24662-24664	IN	denotes	of
T14222	24665-24668	DT	denotes	the
T14224	24669-24675	JJ	denotes	aortic
T14223	24676-24680	NN	denotes	arch
T14225	24680-24681	.	denotes	.
T14226	24681-24927	sentence	denotes	These observed differences are likely due to substantial apoptosis of Alk5-deficient post-migratory neural crest cells, which is clearly detectable at E10.5, whereas similar intense cell death has not been reported in Tgfbr2/Wnt1-Cre mutants[9].
T14227	24682-24687	DT	denotes	These
T14229	24688-24696	VBN	denotes	observed
T14228	24697-24708	NNS	denotes	differences
T14230	24709-24712	VBP	denotes	are
T14231	24713-24719	RB	denotes	likely
T14232	24720-24723	IN	denotes	due
T14233	24724-24726	IN	denotes	to
T14234	24727-24738	JJ	denotes	substantial
T14235	24739-24748	NN	denotes	apoptosis
T14236	24749-24751	IN	denotes	of
T14237	24752-24756	NN	denotes	Alk5
T14239	24756-24757	HYPH	denotes	-
T14238	24757-24766	JJ	denotes	deficient
T14241	24767-24781	JJ	denotes	post-migratory
T14242	24782-24788	JJ	denotes	neural
T14243	24789-24794	NN	denotes	crest
T14240	24795-24800	NNS	denotes	cells
T14244	24800-24802	,	denotes	,
T14245	24802-24807	WDT	denotes	which
T14246	24808-24810	VBZ	denotes	is
T14247	24811-24818	RB	denotes	clearly
T14248	24819-24829	JJ	denotes	detectable
T14249	24830-24832	IN	denotes	at
T14250	24833-24838	NN	denotes	E10.5
T14251	24838-24840	,	denotes	,
T14252	24840-24847	IN	denotes	whereas
T14254	24848-24855	JJ	denotes	similar
T14256	24856-24863	JJ	denotes	intense
T14257	24864-24868	NN	denotes	cell
T14255	24869-24874	NN	denotes	death
T14258	24875-24878	VBZ	denotes	has
T14259	24879-24882	RB	denotes	not
T14260	24883-24887	VBN	denotes	been
T14253	24888-24896	VBN	denotes	reported
T14261	24897-24899	IN	denotes	in
T14262	24900-24906	NN	denotes	Tgfbr2
T14264	24906-24907	HYPH	denotes	/
T14265	24907-24911	NN	denotes	Wnt1
T14266	24911-24912	HYPH	denotes	-
T14263	24912-24915	NN	denotes	Cre
T14267	24916-24923	NNS	denotes	mutants
T14268	24923-24924	-LRB-	denotes	[
T14269	24924-24925	CD	denotes	9
T14270	24925-24926	-RRB-	denotes	]
T14271	24926-24927	.	denotes	.
T14272	24927-25198	sentence	denotes	Our present results suggest that while TGF-β signaling in cardiac NCCs is predominantly mediated via the ALK5/TGF-βRII receptor complex, ALK5 also mediates signaling of other related ligands, which are either directly or indirectly required for appropriate NCC survival.
T14273	24928-24931	PRP$	denotes	Our
T14275	24932-24939	JJ	denotes	present
T14274	24940-24947	NNS	denotes	results
T14276	24948-24955	VBP	denotes	suggest
T14277	24956-24960	IN	denotes	that
T14279	24961-24966	IN	denotes	while
T14281	24967-24970	NN	denotes	TGF
T14283	24970-24971	HYPH	denotes	-
T14282	24971-24972	NN	denotes	β
T14284	24973-24982	NN	denotes	signaling
T14285	24983-24985	IN	denotes	in
T14286	24986-24993	JJ	denotes	cardiac
T14287	24994-24998	NNS	denotes	NCCs
T14288	24999-25001	VBZ	denotes	is
T14289	25002-25015	RB	denotes	predominantly
T14280	25016-25024	VBN	denotes	mediated
T14290	25025-25028	IN	denotes	via
T14291	25029-25032	DT	denotes	the
T14293	25033-25037	NN	denotes	ALK5
T14295	25037-25038	HYPH	denotes	/
T14296	25038-25041	NN	denotes	TGF
T14297	25041-25042	HYPH	denotes	-
T14294	25042-25046	NN	denotes	βRII
T14298	25047-25055	NN	denotes	receptor
T14292	25056-25063	NN	denotes	complex
T14299	25063-25065	,	denotes	,
T14300	25065-25069	NN	denotes	ALK5
T14301	25070-25074	RB	denotes	also
T14278	25075-25083	VBZ	denotes	mediates
T14302	25084-25093	NN	denotes	signaling
T14303	25094-25096	IN	denotes	of
T14304	25097-25102	JJ	denotes	other
T14306	25103-25110	JJ	denotes	related
T14305	25111-25118	NNS	denotes	ligands
T14307	25118-25120	,	denotes	,
T14308	25120-25125	WDT	denotes	which
T14310	25126-25129	VBP	denotes	are
T14311	25130-25136	CC	denotes	either
T14312	25137-25145	RB	denotes	directly
T14313	25146-25148	CC	denotes	or
T14314	25149-25159	RB	denotes	indirectly
T14309	25160-25168	VBN	denotes	required
T14315	25169-25172	IN	denotes	for
T14316	25173-25184	JJ	denotes	appropriate
T14318	25185-25188	NN	denotes	NCC
T14317	25189-25197	NN	denotes	survival
T14319	25197-25198	.	denotes	.
T14320	25198-25355	sentence	denotes	In fact, it has been shown that, besides TGF-βRII, ALK5 can also form a complex with the Activin type IIB receptor to activate downstream Smads 2/3 [18,31].
T14321	25199-25201	IN	denotes	In
T14323	25202-25206	NN	denotes	fact
T14324	25206-25208	,	denotes	,
T14325	25208-25210	PRP	denotes	it
T14326	25211-25214	VBZ	denotes	has
T14327	25215-25219	VBN	denotes	been
T14322	25220-25225	VBN	denotes	shown
T14328	25226-25230	IN	denotes	that
T14330	25230-25232	,	denotes	,
T14331	25232-25239	IN	denotes	besides
T14332	25240-25243	NN	denotes	TGF
T14334	25243-25244	HYPH	denotes	-
T14333	25244-25248	NN	denotes	βRII
T14335	25248-25250	,	denotes	,
T14336	25250-25254	NN	denotes	ALK5
T14337	25255-25258	MD	denotes	can
T14338	25259-25263	RB	denotes	also
T14329	25264-25268	VB	denotes	form
T14339	25269-25270	DT	denotes	a
T14340	25271-25278	NN	denotes	complex
T14341	25279-25283	IN	denotes	with
T14342	25284-25287	DT	denotes	the
T14344	25288-25295	NN	denotes	Activin
T14345	25296-25300	NN	denotes	type
T14346	25301-25304	NN	denotes	IIB
T14343	25305-25313	NN	denotes	receptor
T14347	25314-25316	TO	denotes	to
T14348	25317-25325	VB	denotes	activate
T14349	25326-25336	JJ	denotes	downstream
T14350	25337-25342	NNS	denotes	Smads
T14351	25343-25344	CD	denotes	2
T14352	25344-25345	HYPH	denotes	/
T14353	25345-25346	CD	denotes	3
T14354	25347-25348	-LRB-	denotes	[
T14356	25348-25350	CD	denotes	18
T14357	25350-25351	,	denotes	,
T14355	25351-25353	CD	denotes	31
T14358	25353-25354	-RRB-	denotes	]
T14359	25354-25355	.	denotes	.
T14360	25355-25512	sentence	denotes	Furthermore, a subset of TGF-β-related growth and differentiation factors (GDFs), e.g., GDF8, GDF9, GDF11 and GDF15 could induce these events [17,18,32,33].
T14361	25356-25367	RB	denotes	Furthermore
T14363	25367-25369	,	denotes	,
T14364	25369-25370	DT	denotes	a
T14365	25371-25377	NN	denotes	subset
T14366	25378-25380	IN	denotes	of
T14367	25381-25384	NN	denotes	TGF
T14369	25384-25385	HYPH	denotes	-
T14368	25385-25386	NN	denotes	β
T14371	25386-25387	HYPH	denotes	-
T14370	25387-25394	VBN	denotes	related
T14373	25395-25401	NN	denotes	growth
T14374	25402-25405	CC	denotes	and
T14375	25406-25421	NN	denotes	differentiation
T14372	25422-25429	NNS	denotes	factors
T14376	25430-25431	-LRB-	denotes	(
T14377	25431-25435	NNS	denotes	GDFs
T14378	25435-25436	-RRB-	denotes	)
T14379	25436-25438	,	denotes	,
T14380	25438-25442	FW	denotes	e.g.
T14382	25442-25444	,	denotes	,
T14381	25444-25448	NN	denotes	GDF8
T14383	25448-25450	,	denotes	,
T14384	25450-25454	NN	denotes	GDF9
T14385	25454-25456	,	denotes	,
T14386	25456-25461	NN	denotes	GDF11
T14387	25462-25465	CC	denotes	and
T14388	25466-25471	NN	denotes	GDF15
T14389	25472-25477	MD	denotes	could
T14362	25478-25484	VB	denotes	induce
T14390	25485-25490	DT	denotes	these
T14391	25491-25497	NNS	denotes	events
T14392	25498-25499	-LRB-	denotes	[
T14394	25499-25501	CD	denotes	17
T14395	25501-25502	,	denotes	,
T14396	25502-25504	CD	denotes	18
T14397	25504-25505	,	denotes	,
T14398	25505-25507	CD	denotes	32
T14399	25507-25508	,	denotes	,
T14393	25508-25510	CD	denotes	33
T14400	25510-25511	-RRB-	denotes	]
T14401	25511-25512	.	denotes	.
T14402	25512-25836	sentence	denotes	Although relevant Gdfs 8, 9 11 and 15 are not expressed in the developing heart, nor do the mice deficient in these Gdfs display developmental cardiac defects, we cannot exclude the possibility that circulating GDFs, perhaps in concert with TGF-βs may contribute to NCC survival during cardiac and pharyngeal morphogenesis.
T14403	25513-25521	IN	denotes	Although
T14405	25522-25530	JJ	denotes	relevant
T14407	25531-25535	NNS	denotes	Gdfs
T14406	25536-25537	CD	denotes	8
T14409	25537-25539	,	denotes	,
T14410	25539-25540	CD	denotes	9
T14411	25541-25543	CD	denotes	11
T14412	25544-25547	CC	denotes	and
T14413	25548-25550	CD	denotes	15
T14414	25551-25554	VBP	denotes	are
T14415	25555-25558	RB	denotes	not
T14408	25559-25568	VBN	denotes	expressed
T14416	25569-25571	IN	denotes	in
T14417	25572-25575	DT	denotes	the
T14419	25576-25586	VBG	denotes	developing
T14418	25587-25592	NN	denotes	heart
T14420	25592-25594	,	denotes	,
T14421	25594-25597	CC	denotes	nor
T14422	25598-25600	VBP	denotes	do
T14423	25601-25604	DT	denotes	the
T14424	25605-25609	NNS	denotes	mice
T14425	25610-25619	JJ	denotes	deficient
T14426	25620-25622	IN	denotes	in
T14427	25623-25628	DT	denotes	these
T14428	25629-25633	NNS	denotes	Gdfs
T14404	25634-25641	VB	denotes	display
T14430	25642-25655	JJ	denotes	developmental
T14432	25656-25663	JJ	denotes	cardiac
T14431	25664-25671	NNS	denotes	defects
T14433	25671-25673	,	denotes	,
T14434	25673-25675	PRP	denotes	we
T14435	25676-25679	MD	denotes	can
T14436	25679-25682	RB	denotes	not
T14429	25683-25690	VB	denotes	exclude
T14437	25691-25694	DT	denotes	the
T14438	25695-25706	NN	denotes	possibility
T14439	25707-25711	IN	denotes	that
T14441	25712-25723	VBG	denotes	circulating
T14442	25724-25728	NNS	denotes	GDFs
T14443	25728-25730	,	denotes	,
T14444	25730-25737	RB	denotes	perhaps
T14445	25738-25740	IN	denotes	in
T14446	25741-25748	NN	denotes	concert
T14447	25749-25753	IN	denotes	with
T14448	25754-25757	NNS	denotes	TGF
T14450	25757-25758	HYPH	denotes	-
T14449	25758-25760	NNS	denotes	βs
T14451	25761-25764	MD	denotes	may
T14440	25765-25775	VB	denotes	contribute
T14452	25776-25778	IN	denotes	to
T14453	25779-25782	NN	denotes	NCC
T14454	25783-25791	NN	denotes	survival
T14455	25792-25798	IN	denotes	during
T14456	25799-25806	JJ	denotes	cardiac
T14458	25807-25810	CC	denotes	and
T14459	25811-25821	JJ	denotes	pharyngeal
T14457	25822-25835	NN	denotes	morphogenesis
T14460	25835-25836	.	denotes	.
T14461	25836-25969	sentence	denotes	We specifically studied apoptosis at the level of the aortic sac, where the AP-septum forms between the origins of 4th and 6th PAAs.
T14462	25837-25839	PRP	denotes	We
T14464	25840-25852	RB	denotes	specifically
T14463	25853-25860	VBD	denotes	studied
T14465	25861-25870	NN	denotes	apoptosis
T14466	25871-25873	IN	denotes	at
T14467	25874-25877	DT	denotes	the
T14468	25878-25883	NN	denotes	level
T14469	25884-25886	IN	denotes	of
T14470	25887-25890	DT	denotes	the
T14472	25891-25897	JJ	denotes	aortic
T14471	25898-25901	NN	denotes	sac
T14473	25901-25903	,	denotes	,
T14474	25903-25908	WRB	denotes	where
T14476	25909-25912	DT	denotes	the
T14478	25913-25915	NN	denotes	AP
T14479	25915-25916	HYPH	denotes	-
T14477	25916-25922	NN	denotes	septum
T14475	25923-25928	VBZ	denotes	forms
T14480	25929-25936	IN	denotes	between
T14481	25937-25940	DT	denotes	the
T14482	25941-25948	NNS	denotes	origins
T14483	25949-25951	IN	denotes	of
T14484	25952-25955	JJ	denotes	4th
T14486	25956-25959	CC	denotes	and
T14487	25960-25963	JJ	denotes	6th
T14485	25964-25968	NNS	denotes	PAAs
T14488	25968-25969	.	denotes	.
T14489	25969-26212	sentence	denotes	Already at E10.5, we could see intense apoptosis among the postmigratory NCCs in the mesenchyme surrounding the aortic sac at the site where the prospective AP septum forms, i.e., this cell death precedes the AP septal defect seen in mutants.
T14490	25970-25977	RB	denotes	Already
T14492	25978-25980	IN	denotes	at
T14493	25981-25986	NN	denotes	E10.5
T14494	25986-25988	,	denotes	,
T14495	25988-25990	PRP	denotes	we
T14496	25991-25996	MD	denotes	could
T14491	25997-26000	VB	denotes	see
T14498	26001-26008	JJ	denotes	intense
T14499	26009-26018	NN	denotes	apoptosis
T14500	26019-26024	IN	denotes	among
T14501	26025-26028	DT	denotes	the
T14503	26029-26042	JJ	denotes	postmigratory
T14502	26043-26047	NNS	denotes	NCCs
T14504	26048-26050	IN	denotes	in
T14505	26051-26054	DT	denotes	the
T14506	26055-26065	NN	denotes	mesenchyme
T14507	26066-26077	VBG	denotes	surrounding
T14508	26078-26081	DT	denotes	the
T14510	26082-26088	JJ	denotes	aortic
T14509	26089-26092	NN	denotes	sac
T14511	26093-26095	IN	denotes	at
T14512	26096-26099	DT	denotes	the
T14513	26100-26104	NN	denotes	site
T14514	26105-26110	WRB	denotes	where
T14516	26111-26114	DT	denotes	the
T14518	26115-26126	JJ	denotes	prospective
T14519	26127-26129	NN	denotes	AP
T14517	26130-26136	NN	denotes	septum
T14515	26137-26142	VBZ	denotes	forms
T14520	26142-26144	,	denotes	,
T14521	26144-26148	FW	denotes	i.e.
T14522	26148-26150	,	denotes	,
T14523	26150-26154	DT	denotes	this
T14525	26155-26159	NN	denotes	cell
T14524	26160-26165	NN	denotes	death
T14497	26166-26174	VBZ	denotes	precedes
T14526	26175-26178	DT	denotes	the
T14528	26179-26181	NN	denotes	AP
T14529	26182-26188	JJ	denotes	septal
T14527	26189-26195	NN	denotes	defect
T14530	26196-26200	VBN	denotes	seen
T14531	26201-26203	IN	denotes	in
T14532	26204-26211	NNS	denotes	mutants
T14533	26211-26212	.	denotes	.
T14534	26212-26412	sentence	denotes	Although some NC-derived cells appeared to be differentiating to smooth muscle cells in the OFT (Fig. 6), we could never detect the AP septum forming in Alk5/Wnt1-Cre mutants between E10.5 and E11.5.
T14535	26213-26221	IN	denotes	Although
T14537	26222-26226	DT	denotes	some
T14539	26227-26229	NN	denotes	NC
T14541	26229-26230	HYPH	denotes	-
T14540	26230-26237	VBN	denotes	derived
T14538	26238-26243	NNS	denotes	cells
T14536	26244-26252	VBD	denotes	appeared
T14543	26253-26255	TO	denotes	to
T14545	26256-26258	VB	denotes	be
T14544	26259-26274	VBG	denotes	differentiating
T14546	26275-26277	IN	denotes	to
T14547	26278-26284	JJ	denotes	smooth
T14549	26285-26291	NN	denotes	muscle
T14548	26292-26297	NNS	denotes	cells
T14550	26298-26300	IN	denotes	in
T14551	26301-26304	DT	denotes	the
T14552	26305-26308	NN	denotes	OFT
T14553	26309-26310	-LRB-	denotes	(
T14554	26310-26314	NN	denotes	Fig.
T14555	26315-26316	CD	denotes	6
T14556	26316-26317	-RRB-	denotes	)
T14557	26317-26319	,	denotes	,
T14558	26319-26321	PRP	denotes	we
T14559	26322-26327	MD	denotes	could
T14560	26328-26333	RB	denotes	never
T14542	26334-26340	VB	denotes	detect
T14561	26341-26344	DT	denotes	the
T14563	26345-26347	NN	denotes	AP
T14562	26348-26354	NN	denotes	septum
T14564	26355-26362	VBG	denotes	forming
T14565	26363-26365	IN	denotes	in
T14566	26366-26370	NN	denotes	Alk5
T14568	26370-26371	HYPH	denotes	/
T14569	26371-26375	NN	denotes	Wnt1
T14570	26375-26376	HYPH	denotes	-
T14567	26376-26379	NN	denotes	Cre
T14571	26380-26387	NNS	denotes	mutants
T14572	26388-26395	IN	denotes	between
T14573	26396-26401	NN	denotes	E10.5
T14574	26402-26405	CC	denotes	and
T14575	26406-26411	NN	denotes	E11.5
T14576	26411-26412	.	denotes	.
T14577	26412-26520	sentence	denotes	These findings suggest that the pool of cells forming the AP septum is severely affected by the cell death.
T14578	26413-26418	DT	denotes	These
T14579	26419-26427	NNS	denotes	findings
T14580	26428-26435	VBP	denotes	suggest
T14581	26436-26440	IN	denotes	that
T14583	26441-26444	DT	denotes	the
T14584	26445-26449	NN	denotes	pool
T14585	26450-26452	IN	denotes	of
T14586	26453-26458	NNS	denotes	cells
T14587	26459-26466	VBG	denotes	forming
T14588	26467-26470	DT	denotes	the
T14590	26471-26473	NN	denotes	AP
T14589	26474-26480	NN	denotes	septum
T14591	26481-26483	VBZ	denotes	is
T14592	26484-26492	RB	denotes	severely
T14582	26493-26501	VBN	denotes	affected
T14593	26502-26504	IN	denotes	by
T14594	26505-26508	DT	denotes	the
T14596	26509-26513	NN	denotes	cell
T14595	26514-26519	NN	denotes	death
T14597	26519-26520	.	denotes	.
T14598	26520-26656	sentence	denotes	Moreover, it is likely that these cells forming the AP-septum die the before majority of them can differentiate to smooth muscle cells.
T14599	26521-26529	RB	denotes	Moreover
T14601	26529-26531	,	denotes	,
T14602	26531-26533	PRP	denotes	it
T14600	26534-26536	VBZ	denotes	is
T14603	26537-26543	JJ	denotes	likely
T14604	26544-26548	IN	denotes	that
T14606	26549-26554	DT	denotes	these
T14607	26555-26560	NNS	denotes	cells
T14608	26561-26568	VBG	denotes	forming
T14609	26569-26572	DT	denotes	the
T14611	26573-26575	NN	denotes	AP
T14612	26575-26576	HYPH	denotes	-
T14610	26576-26582	NN	denotes	septum
T14605	26583-26586	VBP	denotes	die
T14613	26587-26590	DT	denotes	the
T14614	26591-26597	IN	denotes	before
T14616	26598-26606	NN	denotes	majority
T14617	26607-26609	IN	denotes	of
T14618	26610-26614	PRP	denotes	them
T14619	26615-26618	MD	denotes	can
T14615	26619-26632	VB	denotes	differentiate
T14620	26633-26635	IN	denotes	to
T14621	26636-26642	JJ	denotes	smooth
T14623	26643-26649	NN	denotes	muscle
T14622	26650-26655	NNS	denotes	cells
T14624	26655-26656	.	denotes	.
T14625	26656-26791	sentence	denotes	Several in vitro studies have suggested an indispensable role for TGF-β-signaling in differentiation of NCCs into smooth muscle cells.
T14626	26657-26664	JJ	denotes	Several
T14628	26665-26667	FW	denotes	in
T14629	26668-26673	FW	denotes	vitro
T14627	26674-26681	NNS	denotes	studies
T14631	26682-26686	VBP	denotes	have
T14630	26687-26696	VBN	denotes	suggested
T14632	26697-26699	DT	denotes	an
T14634	26700-26713	JJ	denotes	indispensable
T14633	26714-26718	NN	denotes	role
T14635	26719-26722	IN	denotes	for
T14636	26723-26726	NN	denotes	TGF
T14638	26726-26727	HYPH	denotes	-
T14637	26727-26728	NN	denotes	β
T14639	26728-26729	HYPH	denotes	-
T14640	26729-26738	VBG	denotes	signaling
T14641	26739-26741	IN	denotes	in
T14642	26742-26757	NN	denotes	differentiation
T14643	26758-26760	IN	denotes	of
T14644	26761-26765	NNS	denotes	NCCs
T14645	26766-26770	IN	denotes	into
T14646	26771-26777	JJ	denotes	smooth
T14647	26778-26784	NN	denotes	muscle
T14648	26785-26790	NNS	denotes	cells
T14649	26790-26791	.	denotes	.
T14650	26791-27015	sentence	denotes	Moreover, a recent in vivo study suggested that mice lacking Tgfbr2 in CNCCs display defective NCC differentiation into αSMA-positive cells in the AP septum [9], although this result was later disputed by another study [8].
T14651	26792-26800	RB	denotes	Moreover
T14653	26800-26802	,	denotes	,
T14654	26802-26803	DT	denotes	a
T14656	26804-26810	JJ	denotes	recent
T14657	26811-26813	FW	denotes	in
T14658	26814-26818	FW	denotes	vivo
T14655	26819-26824	NN	denotes	study
T14652	26825-26834	VBD	denotes	suggested
T14659	26835-26839	IN	denotes	that
T14661	26840-26844	NNS	denotes	mice
T14662	26845-26852	VBG	denotes	lacking
T14663	26853-26859	NN	denotes	Tgfbr2
T14664	26860-26862	IN	denotes	in
T14665	26863-26868	NNS	denotes	CNCCs
T14660	26869-26876	VBP	denotes	display
T14666	26877-26886	JJ	denotes	defective
T14668	26887-26890	NN	denotes	NCC
T14667	26891-26906	NN	denotes	differentiation
T14669	26907-26911	IN	denotes	into
T14670	26912-26916	NN	denotes	αSMA
T14672	26916-26917	HYPH	denotes	-
T14671	26917-26925	JJ	denotes	positive
T14673	26926-26931	NNS	denotes	cells
T14674	26932-26934	IN	denotes	in
T14675	26935-26938	DT	denotes	the
T14677	26939-26941	NN	denotes	AP
T14676	26942-26948	NN	denotes	septum
T14678	26949-26950	-LRB-	denotes	[
T14679	26950-26951	CD	denotes	9
T14680	26951-26952	-RRB-	denotes	]
T14681	26952-26954	,	denotes	,
T14682	26954-26962	IN	denotes	although
T14684	26963-26967	DT	denotes	this
T14685	26968-26974	NN	denotes	result
T14686	26975-26978	VBD	denotes	was
T14687	26979-26984	RB	denotes	later
T14683	26985-26993	VBN	denotes	disputed
T14688	26994-26996	IN	denotes	by
T14689	26997-27004	DT	denotes	another
T14690	27005-27010	NN	denotes	study
T14691	27011-27012	-LRB-	denotes	[
T14692	27012-27013	CD	denotes	8
T14693	27013-27014	-RRB-	denotes	]
T14694	27014-27015	.	denotes	.
T14695	27015-27177	sentence	denotes	Our immunohistochemical staining of αSMA in the OFT unequivocally demonstrated that signaling via ALK5 is not required for smooth muscle differentiation in vivo.
T14696	27016-27019	PRP$	denotes	Our
T14698	27020-27039	JJ	denotes	immunohistochemical
T14697	27040-27048	NN	denotes	staining
T14700	27049-27051	IN	denotes	of
T14701	27052-27056	NN	denotes	αSMA
T14702	27057-27059	IN	denotes	in
T14703	27060-27063	DT	denotes	the
T14704	27064-27067	NN	denotes	OFT
T14705	27068-27081	RB	denotes	unequivocally
T14699	27082-27094	VBD	denotes	demonstrated
T14706	27095-27099	IN	denotes	that
T14708	27100-27109	NN	denotes	signaling
T14709	27110-27113	IN	denotes	via
T14710	27114-27118	NN	denotes	ALK5
T14711	27119-27121	VBZ	denotes	is
T14712	27122-27125	RB	denotes	not
T14707	27126-27134	VBN	denotes	required
T14713	27135-27138	IN	denotes	for
T14714	27139-27145	JJ	denotes	smooth
T14715	27146-27152	NN	denotes	muscle
T14716	27153-27168	NN	denotes	differentiation
T14717	27169-27171	FW	denotes	in
T14718	27172-27176	FW	denotes	vivo
T14719	27176-27177	.	denotes	.
T14720	27177-27457	sentence	denotes	Moreover, it has been suggested that deletion of Tgfbr2 in NCCs leads to other phenotypic features reminiscent of those seen in the velocardiofacial/DiGeorge syndrome (VCF/DGS) [9] caused by a deletion of the so called DiGeorge critical region (DGCR) on chromosome 22q11 [34,35].
T14721	27178-27186	RB	denotes	Moreover
T14723	27186-27188	,	denotes	,
T14724	27188-27190	PRP	denotes	it
T14725	27191-27194	VBZ	denotes	has
T14726	27195-27199	VBN	denotes	been
T14722	27200-27209	VBN	denotes	suggested
T14727	27210-27214	IN	denotes	that
T14729	27215-27223	NN	denotes	deletion
T14730	27224-27226	IN	denotes	of
T14731	27227-27233	NN	denotes	Tgfbr2
T14732	27234-27236	IN	denotes	in
T14733	27237-27241	NNS	denotes	NCCs
T14728	27242-27247	VBZ	denotes	leads
T14734	27248-27250	IN	denotes	to
T14735	27251-27256	JJ	denotes	other
T14737	27257-27267	JJ	denotes	phenotypic
T14736	27268-27276	NNS	denotes	features
T14738	27277-27288	JJ	denotes	reminiscent
T14739	27289-27291	IN	denotes	of
T14740	27292-27297	DT	denotes	those
T14741	27298-27302	VBN	denotes	seen
T14742	27303-27305	IN	denotes	in
T14743	27306-27309	DT	denotes	the
T14745	27310-27326	JJ	denotes	velocardiofacial
T14747	27326-27327	HYPH	denotes	/
T14746	27327-27335	NN	denotes	DiGeorge
T14744	27336-27344	NN	denotes	syndrome
T14748	27345-27346	-LRB-	denotes	(
T14749	27346-27349	NN	denotes	VCF
T14751	27349-27350	HYPH	denotes	/
T14750	27350-27353	NN	denotes	DGS
T14752	27353-27354	-RRB-	denotes	)
T14753	27355-27356	-LRB-	denotes	[
T14754	27356-27357	CD	denotes	9
T14755	27357-27358	-RRB-	denotes	]
T14756	27359-27365	VBN	denotes	caused
T14757	27366-27368	IN	denotes	by
T14758	27369-27370	DT	denotes	a
T14759	27371-27379	NN	denotes	deletion
T14760	27380-27382	IN	denotes	of
T14761	27383-27386	DT	denotes	the
T14763	27387-27389	RB	denotes	so
T14764	27390-27396	VBN	denotes	called
T14765	27397-27405	NNP	denotes	DiGeorge
T14766	27406-27414	JJ	denotes	critical
T14762	27415-27421	NN	denotes	region
T14767	27422-27423	-LRB-	denotes	(
T14768	27423-27427	NN	denotes	DGCR
T14769	27427-27428	-RRB-	denotes	)
T14770	27429-27431	IN	denotes	on
T14771	27432-27442	NN	denotes	chromosome
T14772	27443-27448	NN	denotes	22q11
T14773	27449-27450	-LRB-	denotes	[
T14775	27450-27452	CD	denotes	34
T14776	27452-27453	,	denotes	,
T14774	27453-27455	CD	denotes	35
T14777	27455-27456	-RRB-	denotes	]
T14778	27456-27457	.	denotes	.
T14779	27457-27718	sentence	denotes	Our present results suggest that although many of the observed phenotypes seen in Alk5/Wnt1-Cre mutants superficially resemble those seen in VCF/DGS, a detailed examination shows that the NC-specific abrogation of Alk5 does not lead to VCF/DGS-like phenotypes.
T14780	27458-27461	PRP$	denotes	Our
T14782	27462-27469	JJ	denotes	present
T14781	27470-27477	NNS	denotes	results
T14783	27478-27485	VBP	denotes	suggest
T14784	27486-27490	IN	denotes	that
T14786	27491-27499	IN	denotes	although
T14788	27500-27504	JJ	denotes	many
T14789	27505-27507	IN	denotes	of
T14790	27508-27511	DT	denotes	the
T14792	27512-27520	VBN	denotes	observed
T14791	27521-27531	NNS	denotes	phenotypes
T14793	27532-27536	VBN	denotes	seen
T14794	27537-27539	IN	denotes	in
T14795	27540-27544	NN	denotes	Alk5
T14797	27544-27545	HYPH	denotes	/
T14798	27545-27549	NN	denotes	Wnt1
T14799	27549-27550	HYPH	denotes	-
T14796	27550-27553	NN	denotes	Cre
T14800	27554-27561	NNS	denotes	mutants
T14801	27562-27575	RB	denotes	superficially
T14787	27576-27584	VBP	denotes	resemble
T14802	27585-27590	DT	denotes	those
T14803	27591-27595	VBN	denotes	seen
T14804	27596-27598	IN	denotes	in
T14805	27599-27602	NN	denotes	VCF
T14807	27602-27603	HYPH	denotes	/
T14806	27603-27606	NN	denotes	DGS
T14808	27606-27608	,	denotes	,
T14809	27608-27609	DT	denotes	a
T14811	27610-27618	VBN	denotes	detailed
T14810	27619-27630	NN	denotes	examination
T14785	27631-27636	VBZ	denotes	shows
T14812	27637-27641	IN	denotes	that
T14814	27642-27645	DT	denotes	the
T14816	27646-27648	NN	denotes	NC
T14818	27648-27649	HYPH	denotes	-
T14817	27649-27657	JJ	denotes	specific
T14815	27658-27668	NN	denotes	abrogation
T14819	27669-27671	IN	denotes	of
T14820	27672-27676	NN	denotes	Alk5
T14821	27677-27681	VBZ	denotes	does
T14822	27682-27685	RB	denotes	not
T14813	27686-27690	VB	denotes	lead
T14823	27691-27693	IN	denotes	to
T14824	27694-27697	NN	denotes	VCF
T14826	27697-27698	HYPH	denotes	/
T14825	27698-27701	NN	denotes	DGS
T14828	27701-27702	HYPH	denotes	-
T14827	27702-27706	JJ	denotes	like
T14829	27707-27717	NNS	denotes	phenotypes
T14830	27717-27718	.	denotes	.
T14831	27718-27999	sentence	denotes	Firstly, while the pharyngeal organ migration fails in Alk5/Wnt1-Cre mutants, perhaps as a result of increased mesenchymal cell death in the pharyngeal region, both the thymus, thyroid and parathyroid seem to develop relatively normally on the histological level in these mutants.
T14832	27719-27726	RB	denotes	Firstly
T14834	27726-27728	,	denotes	,
T14835	27728-27733	IN	denotes	while
T14837	27734-27737	DT	denotes	the
T14839	27738-27748	JJ	denotes	pharyngeal
T14840	27749-27754	NN	denotes	organ
T14838	27755-27764	NN	denotes	migration
T14836	27765-27770	VBZ	denotes	fails
T14841	27771-27773	IN	denotes	in
T14842	27774-27778	NN	denotes	Alk5
T14844	27778-27779	HYPH	denotes	/
T14845	27779-27783	NN	denotes	Wnt1
T14846	27783-27784	HYPH	denotes	-
T14843	27784-27787	NN	denotes	Cre
T14847	27788-27795	NNS	denotes	mutants
T14848	27795-27797	,	denotes	,
T14849	27797-27804	RB	denotes	perhaps
T14850	27805-27807	IN	denotes	as
T14851	27808-27809	DT	denotes	a
T14852	27810-27816	NN	denotes	result
T14853	27817-27819	IN	denotes	of
T14854	27820-27829	VBN	denotes	increased
T14856	27830-27841	JJ	denotes	mesenchymal
T14857	27842-27846	NN	denotes	cell
T14855	27847-27852	NN	denotes	death
T14858	27853-27855	IN	denotes	in
T14859	27856-27859	DT	denotes	the
T14861	27860-27870	JJ	denotes	pharyngeal
T14860	27871-27877	NN	denotes	region
T14862	27877-27879	,	denotes	,
T14863	27879-27883	CC	denotes	both
T14865	27884-27887	DT	denotes	the
T14864	27888-27894	NN	denotes	thymus
T14866	27894-27896	,	denotes	,
T14867	27896-27903	NN	denotes	thyroid
T14868	27904-27907	CC	denotes	and
T14869	27908-27919	NN	denotes	parathyroid
T14833	27920-27924	VBP	denotes	seem
T14870	27925-27927	TO	denotes	to
T14871	27928-27935	VB	denotes	develop
T14872	27936-27946	RB	denotes	relatively
T14873	27947-27955	RB	denotes	normally
T14874	27956-27958	IN	denotes	on
T14875	27959-27962	DT	denotes	the
T14877	27963-27975	JJ	denotes	histological
T14876	27976-27981	NN	denotes	level
T14878	27982-27984	IN	denotes	in
T14879	27985-27990	DT	denotes	these
T14880	27991-27998	NNS	denotes	mutants
T14881	27998-27999	.	denotes	.
T14882	27999-28390	sentence	denotes	Secondly, the NCC death seen in Alk5 mutants affects a predominantly postmigratory population of NCCs, while genes located in the DGCR, i.e., Tbx1 and CrkL, control NCC survival earlier at E8.5-E10 by regulating proliferation of the secondary heart field (SHF), and endoderm expansion, which in turn provides survival signal for NCCs allowing them to populate the pharyngeal region [36-39].
T14883	28000-28008	RB	denotes	Secondly
T14885	28008-28010	,	denotes	,
T14886	28010-28013	DT	denotes	the
T14888	28014-28017	NN	denotes	NCC
T14887	28018-28023	NN	denotes	death
T14889	28024-28028	VBN	denotes	seen
T14890	28029-28031	IN	denotes	in
T14891	28032-28036	NN	denotes	Alk5
T14892	28037-28044	NNS	denotes	mutants
T14884	28045-28052	VBZ	denotes	affects
T14893	28053-28054	DT	denotes	a
T14895	28055-28068	RB	denotes	predominantly
T14896	28069-28082	JJ	denotes	postmigratory
T14894	28083-28093	NN	denotes	population
T14897	28094-28096	IN	denotes	of
T14898	28097-28101	NNS	denotes	NCCs
T14899	28101-28103	,	denotes	,
T14900	28103-28108	IN	denotes	while
T14902	28109-28114	NNS	denotes	genes
T14903	28115-28122	VBN	denotes	located
T14904	28123-28125	IN	denotes	in
T14905	28126-28129	DT	denotes	the
T14906	28130-28134	NN	denotes	DGCR
T14907	28134-28136	,	denotes	,
T14908	28136-28140	FW	denotes	i.e.
T14910	28140-28142	,	denotes	,
T14909	28142-28146	NN	denotes	Tbx1
T14911	28147-28150	CC	denotes	and
T14912	28151-28155	NN	denotes	CrkL
T14913	28155-28157	,	denotes	,
T14901	28157-28164	VBP	denotes	control
T14914	28165-28168	NN	denotes	NCC
T14915	28169-28177	NN	denotes	survival
T14916	28178-28185	RB	denotes	earlier
T14917	28186-28188	IN	denotes	at
T14918	28189-28193	NN	denotes	E8.5
T14919	28193-28194	SYM	denotes	-
T14920	28194-28197	NN	denotes	E10
T14921	28198-28200	IN	denotes	by
T14922	28201-28211	VBG	denotes	regulating
T14923	28212-28225	NN	denotes	proliferation
T14924	28226-28228	IN	denotes	of
T14925	28229-28232	DT	denotes	the
T14927	28233-28242	JJ	denotes	secondary
T14928	28243-28248	NN	denotes	heart
T14926	28249-28254	NN	denotes	field
T14929	28255-28256	-LRB-	denotes	(
T14930	28256-28259	NN	denotes	SHF
T14931	28259-28260	-RRB-	denotes	)
T14932	28260-28262	,	denotes	,
T14933	28262-28265	CC	denotes	and
T14934	28266-28274	NN	denotes	endoderm
T14935	28275-28284	NN	denotes	expansion
T14936	28284-28286	,	denotes	,
T14937	28286-28291	WDT	denotes	which
T14939	28292-28294	IN	denotes	in
T14940	28295-28299	NN	denotes	turn
T14938	28300-28308	VBZ	denotes	provides
T14941	28309-28317	NN	denotes	survival
T14942	28318-28324	NN	denotes	signal
T14943	28325-28328	IN	denotes	for
T14944	28329-28333	NNS	denotes	NCCs
T14945	28334-28342	VBG	denotes	allowing
T14946	28343-28347	PRP	denotes	them
T14947	28348-28350	TO	denotes	to
T14948	28351-28359	VB	denotes	populate
T14949	28360-28363	DT	denotes	the
T14951	28364-28374	JJ	denotes	pharyngeal
T14950	28375-28381	NN	denotes	region
T14952	28382-28383	-LRB-	denotes	[
T14953	28383-28385	CD	denotes	36
T14954	28385-28386	SYM	denotes	-
T14955	28386-28388	CD	denotes	39
T14956	28388-28389	-RRB-	denotes	]
T14957	28389-28390	.	denotes	.
T14958	28390-28526	sentence	denotes	NCC ablation in the chick has been shown to lead to PTA and to a failure of addition of myocardium from the secondary heart field [40].
T14959	28391-28394	NN	denotes	NCC
T14960	28395-28403	NN	denotes	ablation
T14962	28404-28406	IN	denotes	in
T14963	28407-28410	DT	denotes	the
T14964	28411-28416	NN	denotes	chick
T14965	28417-28420	VBZ	denotes	has
T14966	28421-28425	VBN	denotes	been
T14961	28426-28431	VBN	denotes	shown
T14967	28432-28434	TO	denotes	to
T14968	28435-28439	VB	denotes	lead
T14969	28440-28442	IN	denotes	to
T14970	28443-28446	NN	denotes	PTA
T14971	28447-28450	CC	denotes	and
T14972	28451-28453	IN	denotes	to
T14973	28454-28455	DT	denotes	a
T14974	28456-28463	NN	denotes	failure
T14975	28464-28466	IN	denotes	of
T14976	28467-28475	NN	denotes	addition
T14977	28476-28478	IN	denotes	of
T14978	28479-28489	NN	denotes	myocardium
T14979	28490-28494	IN	denotes	from
T14980	28495-28498	DT	denotes	the
T14982	28499-28508	JJ	denotes	secondary
T14983	28509-28514	NN	denotes	heart
T14981	28515-28520	NN	denotes	field
T14984	28521-28522	-LRB-	denotes	[
T14985	28522-28524	CD	denotes	40
T14986	28524-28525	-RRB-	denotes	]
T14987	28525-28526	.	denotes	.
T14988	28526-28765	sentence	denotes	It was suggested that the defective migration of cells from the secondary heart field to the OFT in turn resulted in shortening and inappropriate rotation of the OFT, leading to mal-alignment of the arterial pole with the ventricles [41].
T14989	28527-28529	PRP	denotes	It
T14991	28530-28533	VBD	denotes	was
T14990	28534-28543	VBN	denotes	suggested
T14992	28544-28548	IN	denotes	that
T14994	28549-28552	DT	denotes	the
T14996	28553-28562	JJ	denotes	defective
T14995	28563-28572	NN	denotes	migration
T14997	28573-28575	IN	denotes	of
T14998	28576-28581	NNS	denotes	cells
T14999	28582-28586	IN	denotes	from
T15000	28587-28590	DT	denotes	the
T15002	28591-28600	JJ	denotes	secondary
T15003	28601-28606	NN	denotes	heart
T15001	28607-28612	NN	denotes	field
T15004	28613-28615	IN	denotes	to
T15005	28616-28619	DT	denotes	the
T15006	28620-28623	NN	denotes	OFT
T15007	28624-28626	IN	denotes	in
T15008	28627-28631	NN	denotes	turn
T14993	28632-28640	VBD	denotes	resulted
T15009	28641-28643	IN	denotes	in
T15010	28644-28654	NN	denotes	shortening
T15011	28655-28658	CC	denotes	and
T15012	28659-28672	JJ	denotes	inappropriate
T15013	28673-28681	NN	denotes	rotation
T15014	28682-28684	IN	denotes	of
T15015	28685-28688	DT	denotes	the
T15016	28689-28692	NN	denotes	OFT
T15017	28692-28694	,	denotes	,
T15018	28694-28701	VBG	denotes	leading
T15019	28702-28704	IN	denotes	to
T15020	28705-28718	NN	denotes	mal-alignment
T15021	28719-28721	IN	denotes	of
T15022	28722-28725	DT	denotes	the
T15024	28726-28734	JJ	denotes	arterial
T15023	28735-28739	NN	denotes	pole
T15025	28740-28744	IN	denotes	with
T15026	28745-28748	DT	denotes	the
T15027	28749-28759	NNS	denotes	ventricles
T15028	28760-28761	-LRB-	denotes	[
T15029	28761-28763	CD	denotes	41
T15030	28763-28764	-RRB-	denotes	]
T15031	28764-28765	.	denotes	.
T15032	28765-29056	sentence	denotes	While the detected OFT phenotype in Alk5/Wnt1-Cre mutants shared many similarities with that seen in the chick NC ablation models, e.g., PTA and the hypoplastic aortic sac, our current results suggest that the secondary heart field is not severely affected in Alk5 mutants (data not shown).
T15033	28766-28771	IN	denotes	While
T15035	28772-28775	DT	denotes	the
T15037	28776-28784	VBN	denotes	detected
T15038	28785-28788	NN	denotes	OFT
T15036	28789-28798	NN	denotes	phenotype
T15039	28799-28801	IN	denotes	in
T15040	28802-28806	NN	denotes	Alk5
T15042	28806-28807	HYPH	denotes	/
T15043	28807-28811	NN	denotes	Wnt1
T15044	28811-28812	HYPH	denotes	-
T15041	28812-28815	NN	denotes	Cre
T15045	28816-28823	NNS	denotes	mutants
T15034	28824-28830	VBD	denotes	shared
T15047	28831-28835	JJ	denotes	many
T15048	28836-28848	NNS	denotes	similarities
T15049	28849-28853	IN	denotes	with
T15050	28854-28858	DT	denotes	that
T15051	28859-28863	VBN	denotes	seen
T15052	28864-28866	IN	denotes	in
T15053	28867-28870	DT	denotes	the
T15055	28871-28876	NN	denotes	chick
T15056	28877-28879	NN	denotes	NC
T15057	28880-28888	NN	denotes	ablation
T15054	28889-28895	NNS	denotes	models
T15058	28895-28897	,	denotes	,
T15059	28897-28901	FW	denotes	e.g.
T15061	28901-28903	,	denotes	,
T15060	28903-28906	NN	denotes	PTA
T15062	28907-28910	CC	denotes	and
T15063	28911-28914	DT	denotes	the
T15065	28915-28926	JJ	denotes	hypoplastic
T15066	28927-28933	JJ	denotes	aortic
T15064	28934-28937	NN	denotes	sac
T15067	28937-28939	,	denotes	,
T15068	28939-28942	PRP$	denotes	our
T15070	28943-28950	JJ	denotes	current
T15069	28951-28958	NNS	denotes	results
T15046	28959-28966	VBP	denotes	suggest
T15071	28967-28971	IN	denotes	that
T15073	28972-28975	DT	denotes	the
T15075	28976-28985	JJ	denotes	secondary
T15076	28986-28991	NN	denotes	heart
T15074	28992-28997	NN	denotes	field
T15077	28998-29000	VBZ	denotes	is
T15078	29001-29004	RB	denotes	not
T15079	29005-29013	RB	denotes	severely
T15072	29014-29022	VBN	denotes	affected
T15080	29023-29025	IN	denotes	in
T15081	29026-29030	NN	denotes	Alk5
T15082	29031-29038	NNS	denotes	mutants
T15083	29039-29040	-LRB-	denotes	(
T15085	29040-29044	NNS	denotes	data
T15086	29045-29048	RB	denotes	not
T15084	29049-29054	VBN	denotes	shown
T15087	29054-29055	-RRB-	denotes	)
T15088	29055-29056	.	denotes	.
T15089	29056-29293	sentence	denotes	Since we could not detect appropriate rotation of the OFT in neural crest-specific mutants of Alk5 or Alk2, it appears that cells derived from the NC, as well as those from the SHF, are mutually required for proper OFT rotation in mice.
T15090	29057-29062	IN	denotes	Since
T15092	29063-29065	PRP	denotes	we
T15093	29066-29071	MD	denotes	could
T15094	29072-29075	RB	denotes	not
T15091	29076-29082	VB	denotes	detect
T15096	29083-29094	JJ	denotes	appropriate
T15097	29095-29103	NN	denotes	rotation
T15098	29104-29106	IN	denotes	of
T15099	29107-29110	DT	denotes	the
T15100	29111-29114	NN	denotes	OFT
T15101	29115-29117	IN	denotes	in
T15102	29118-29124	JJ	denotes	neural
T15103	29125-29130	NN	denotes	crest
T15105	29130-29131	HYPH	denotes	-
T15104	29131-29139	JJ	denotes	specific
T15106	29140-29147	NNS	denotes	mutants
T15107	29148-29150	IN	denotes	of
T15108	29151-29155	NN	denotes	Alk5
T15109	29156-29158	CC	denotes	or
T15110	29159-29163	NN	denotes	Alk2
T15111	29163-29165	,	denotes	,
T15112	29165-29167	PRP	denotes	it
T15095	29168-29175	VBZ	denotes	appears
T15113	29176-29180	IN	denotes	that
T15115	29181-29186	NNS	denotes	cells
T15116	29187-29194	VBN	denotes	derived
T15117	29195-29199	IN	denotes	from
T15118	29200-29203	DT	denotes	the
T15119	29204-29206	NN	denotes	NC
T15120	29206-29208	,	denotes	,
T15121	29208-29210	RB	denotes	as
T15123	29211-29215	RB	denotes	well
T15122	29216-29218	IN	denotes	as
T15124	29219-29224	DT	denotes	those
T15125	29225-29229	IN	denotes	from
T15126	29230-29233	DT	denotes	the
T15127	29234-29237	NN	denotes	SHF
T15128	29237-29239	,	denotes	,
T15129	29239-29242	VBP	denotes	are
T15130	29243-29251	RB	denotes	mutually
T15114	29252-29260	VBN	denotes	required
T15131	29261-29264	IN	denotes	for
T15132	29265-29271	JJ	denotes	proper
T15134	29272-29275	NN	denotes	OFT
T15133	29276-29284	NN	denotes	rotation
T15135	29285-29287	IN	denotes	in
T15136	29288-29292	NNS	denotes	mice
T15137	29292-29293	.	denotes	.
T15138	29293-29412	sentence	denotes	However, it appears that these two TGF-β/BMP type I receptors contribute to OFT rotation through different mechanisms.
T15139	29294-29301	RB	denotes	However
T15141	29301-29303	,	denotes	,
T15142	29303-29305	PRP	denotes	it
T15140	29306-29313	VBZ	denotes	appears
T15143	29314-29318	IN	denotes	that
T15145	29319-29324	DT	denotes	these
T15147	29325-29328	CD	denotes	two
T15148	29329-29332	NN	denotes	TGF
T15150	29332-29333	HYPH	denotes	-
T15151	29333-29334	NN	denotes	β
T15152	29334-29335	HYPH	denotes	/
T15149	29335-29338	NN	denotes	BMP
T15153	29339-29343	NN	denotes	type
T15154	29344-29345	CD	denotes	I
T15146	29346-29355	NNS	denotes	receptors
T15144	29356-29366	VBP	denotes	contribute
T15155	29367-29369	IN	denotes	to
T15156	29370-29373	NN	denotes	OFT
T15157	29374-29382	NN	denotes	rotation
T15158	29383-29390	IN	denotes	through
T15159	29391-29400	JJ	denotes	different
T15160	29401-29411	NNS	denotes	mechanisms
T15161	29411-29412	.	denotes	.
T15162	29412-29640	sentence	denotes	In Alk5 mutants there is very little, if any, detectable septal mesenchyme present, and thus it could be argued that in these mutants OFT rotation fails due to a lack of penetration of septal prongs into the cushion mesenchyme.
T15163	29413-29415	IN	denotes	In
T15165	29416-29420	NN	denotes	Alk5
T15166	29421-29428	NNS	denotes	mutants
T15167	29429-29434	EX	denotes	there
T15164	29435-29437	VBZ	denotes	is
T15168	29438-29442	RB	denotes	very
T15169	29443-29449	JJ	denotes	little
T15171	29449-29451	,	denotes	,
T15173	29451-29453	IN	denotes	if
T15172	29454-29457	DT	denotes	any
T15174	29457-29459	,	denotes	,
T15175	29459-29469	JJ	denotes	detectable
T15176	29470-29476	JJ	denotes	septal
T15170	29477-29487	NN	denotes	mesenchyme
T15177	29488-29495	JJ	denotes	present
T15178	29495-29497	,	denotes	,
T15179	29497-29500	CC	denotes	and
T15180	29501-29505	RB	denotes	thus
T15182	29506-29508	PRP	denotes	it
T15183	29509-29514	MD	denotes	could
T15184	29515-29517	VB	denotes	be
T15181	29518-29524	VBN	denotes	argued
T15185	29525-29529	IN	denotes	that
T15187	29530-29532	IN	denotes	in
T15188	29533-29538	DT	denotes	these
T15189	29539-29546	NNS	denotes	mutants
T15190	29547-29550	NN	denotes	OFT
T15191	29551-29559	NN	denotes	rotation
T15186	29560-29565	VBZ	denotes	fails
T15192	29566-29569	IN	denotes	due
T15193	29570-29572	IN	denotes	to
T15194	29573-29574	DT	denotes	a
T15195	29575-29579	NN	denotes	lack
T15196	29580-29582	IN	denotes	of
T15197	29583-29594	NN	denotes	penetration
T15198	29595-29597	IN	denotes	of
T15199	29598-29604	JJ	denotes	septal
T15200	29605-29611	NNS	denotes	prongs
T15201	29612-29616	IN	denotes	into
T15202	29617-29620	DT	denotes	the
T15204	29621-29628	NN	denotes	cushion
T15203	29629-29639	NN	denotes	mesenchyme
T15205	29639-29640	.	denotes	.
T15206	29640-29939	sentence	denotes	In contrast, in Alk2/Wnt1-Cre mutants a sizeable septal mesenchyme could be seen, still without any obvious looping of the aortic sac and truncal OFT, suggesting that the mere presence of the septal mesenchyme, without correct smooth muscle cell differentiation, is not sufficient for OFT rotation.
T15207	29641-29643	IN	denotes	In
T15209	29644-29652	NN	denotes	contrast
T15210	29652-29654	,	denotes	,
T15211	29654-29656	IN	denotes	in
T15212	29657-29661	NN	denotes	Alk2
T15214	29661-29662	HYPH	denotes	/
T15215	29662-29666	NN	denotes	Wnt1
T15216	29666-29667	HYPH	denotes	-
T15213	29667-29670	NN	denotes	Cre
T15217	29671-29678	NNS	denotes	mutants
T15218	29679-29680	DT	denotes	a
T15220	29681-29689	JJ	denotes	sizeable
T15221	29690-29696	JJ	denotes	septal
T15219	29697-29707	NN	denotes	mesenchyme
T15222	29708-29713	MD	denotes	could
T15223	29714-29716	VB	denotes	be
T15208	29717-29721	VBN	denotes	seen
T15224	29721-29723	,	denotes	,
T15225	29723-29728	RB	denotes	still
T15226	29729-29736	IN	denotes	without
T15227	29737-29740	DT	denotes	any
T15229	29741-29748	JJ	denotes	obvious
T15228	29749-29756	NN	denotes	looping
T15230	29757-29759	IN	denotes	of
T15231	29760-29763	DT	denotes	the
T15233	29764-29770	JJ	denotes	aortic
T15232	29771-29774	NN	denotes	sac
T15234	29775-29778	CC	denotes	and
T15235	29779-29786	JJ	denotes	truncal
T15236	29787-29790	NN	denotes	OFT
T15237	29790-29792	,	denotes	,
T15238	29792-29802	VBG	denotes	suggesting
T15239	29803-29807	IN	denotes	that
T15241	29808-29811	DT	denotes	the
T15243	29812-29816	JJ	denotes	mere
T15242	29817-29825	NN	denotes	presence
T15244	29826-29828	IN	denotes	of
T15245	29829-29832	DT	denotes	the
T15247	29833-29839	JJ	denotes	septal
T15246	29840-29850	NN	denotes	mesenchyme
T15248	29850-29852	,	denotes	,
T15249	29852-29859	IN	denotes	without
T15250	29860-29867	JJ	denotes	correct
T15252	29868-29874	JJ	denotes	smooth
T15253	29875-29881	NN	denotes	muscle
T15254	29882-29886	NN	denotes	cell
T15251	29887-29902	NN	denotes	differentiation
T15255	29902-29904	,	denotes	,
T15240	29904-29906	VBZ	denotes	is
T15256	29907-29910	RB	denotes	not
T15257	29911-29921	JJ	denotes	sufficient
T15258	29922-29925	IN	denotes	for
T15259	29926-29929	NN	denotes	OFT
T15260	29930-29938	NN	denotes	rotation
T15261	29938-29939	.	denotes	.
T15539	29952-29954	IN	denotes	In
T15541	29955-29959	DT	denotes	this
T15542	29960-29965	NN	denotes	study
T15543	29965-29967	,	denotes	,
T15544	29967-29969	PRP	denotes	we
T15545	29970-29974	VBP	denotes	have
T15540	29975-29982	VBN	denotes	deleted
T15546	29983-29986	DT	denotes	the
T15548	29987-29990	NN	denotes	TGF
T15550	29990-29991	HYPH	denotes	-
T15549	29991-29992	NN	denotes	β
T15551	29993-29997	NN	denotes	type
T15552	29998-29999	CD	denotes	I
T15553	30000-30008	NN	denotes	receptor
T15554	30009-30010	-LRB-	denotes	(
T15555	30010-30014	NN	denotes	Alk5
T15556	30014-30015	-RRB-	denotes	)
T15547	30016-30020	NN	denotes	gene
T15557	30021-30033	RB	denotes	specifically
T15558	30034-30036	IN	denotes	in
T15559	30037-30040	DT	denotes	the
T15561	30041-30046	NN	denotes	mouse
T15562	30047-30053	JJ	denotes	neural
T15563	30054-30059	NN	denotes	crest
T15564	30060-30061	-LRB-	denotes	(
T15565	30061-30063	NN	denotes	NC
T15566	30063-30064	-RRB-	denotes	)
T15567	30065-30069	NN	denotes	cell
T15560	30070-30077	NN	denotes	lineage
T15568	30077-30078	.	denotes	.
T15569	30078-30275	sentence	denotes	Our data suggest that ALK5 is required cell autonomously in the NC to mediate non-redundant signaling events that are essential for appropriate patterning of the pharyngeal organs and cardiac OFT.
T15570	30079-30082	PRP$	denotes	Our
T15571	30083-30087	NNS	denotes	data
T15572	30088-30095	VBP	denotes	suggest
T15573	30096-30100	IN	denotes	that
T15575	30101-30105	NN	denotes	ALK5
T15576	30106-30108	VBZ	denotes	is
T15574	30109-30117	VBN	denotes	required
T15577	30118-30122	NN	denotes	cell
T15578	30123-30135	RB	denotes	autonomously
T15579	30136-30138	IN	denotes	in
T15580	30139-30142	DT	denotes	the
T15581	30143-30145	NN	denotes	NC
T15582	30146-30148	TO	denotes	to
T15583	30149-30156	VB	denotes	mediate
T15584	30157-30170	JJ	denotes	non-redundant
T15586	30171-30180	NN	denotes	signaling
T15585	30181-30187	NNS	denotes	events
T15587	30188-30192	WDT	denotes	that
T15588	30193-30196	VBP	denotes	are
T15589	30197-30206	JJ	denotes	essential
T15590	30207-30210	IN	denotes	for
T15591	30211-30222	JJ	denotes	appropriate
T15592	30223-30233	NN	denotes	patterning
T15593	30234-30236	IN	denotes	of
T15594	30237-30240	DT	denotes	the
T15596	30241-30251	JJ	denotes	pharyngeal
T15595	30252-30258	NNS	denotes	organs
T15597	30259-30262	CC	denotes	and
T15598	30263-30270	JJ	denotes	cardiac
T15599	30271-30274	NN	denotes	OFT
T15600	30274-30275	.	denotes	.
T15601	30275-30577	sentence	denotes	The cardiac and pharyngeal defects observed in the NC-specific Alk5 mutants differ significantly from those seen in corresponding mutants lacking the TGF-β type II receptor, suggesting that signaling mediated by ALK5 is not limited to the classical TGF-β ligands during cardiac/pharyngeal development.
T15602	30276-30279	DT	denotes	The
T15604	30280-30287	JJ	denotes	cardiac
T15605	30288-30291	CC	denotes	and
T15606	30292-30302	JJ	denotes	pharyngeal
T15603	30303-30310	NNS	denotes	defects
T15608	30311-30319	VBN	denotes	observed
T15609	30320-30322	IN	denotes	in
T15610	30323-30326	DT	denotes	the
T15612	30327-30329	NN	denotes	NC
T15614	30329-30330	HYPH	denotes	-
T15613	30330-30338	JJ	denotes	specific
T15615	30339-30343	NN	denotes	Alk5
T15611	30344-30351	NNS	denotes	mutants
T15607	30352-30358	VBP	denotes	differ
T15616	30359-30372	RB	denotes	significantly
T15617	30373-30377	IN	denotes	from
T15618	30378-30383	DT	denotes	those
T15619	30384-30388	VBN	denotes	seen
T15620	30389-30391	IN	denotes	in
T15621	30392-30405	VBG	denotes	corresponding
T15622	30406-30413	NNS	denotes	mutants
T15623	30414-30421	VBG	denotes	lacking
T15624	30422-30425	DT	denotes	the
T15626	30426-30429	NN	denotes	TGF
T15628	30429-30430	HYPH	denotes	-
T15627	30430-30431	NN	denotes	β
T15629	30432-30436	NN	denotes	type
T15630	30437-30439	CD	denotes	II
T15625	30440-30448	NN	denotes	receptor
T15631	30448-30450	,	denotes	,
T15632	30450-30460	VBG	denotes	suggesting
T15633	30461-30465	IN	denotes	that
T15635	30466-30475	NN	denotes	signaling
T15636	30476-30484	VBN	denotes	mediated
T15637	30485-30487	IN	denotes	by
T15638	30488-30492	NN	denotes	ALK5
T15639	30493-30495	VBZ	denotes	is
T15640	30496-30499	RB	denotes	not
T15634	30500-30507	VBN	denotes	limited
T15641	30508-30510	IN	denotes	to
T15642	30511-30514	DT	denotes	the
T15644	30515-30524	JJ	denotes	classical
T15645	30525-30528	NN	denotes	TGF
T15647	30528-30529	HYPH	denotes	-
T15646	30529-30530	NN	denotes	β
T15643	30531-30538	NNS	denotes	ligands
T15648	30539-30545	IN	denotes	during
T15649	30546-30553	JJ	denotes	cardiac
T15651	30553-30554	HYPH	denotes	/
T15650	30554-30564	JJ	denotes	pharyngeal
T15652	30565-30576	NN	denotes	development
T15653	30576-30577	.	denotes	.
T15914	30588-30592	NN	denotes	Alk5
T15916	30592-30593	HYPH	denotes	/
T15915	30593-30600	NN	denotes	Wnt1Cre
T15917	30601-30605	NNS	denotes	mice
T15918	30605-30800	sentence	denotes	Alk5 (and Alk2) mutant and control embryos were generated by mating Alk5ko/+(Alk2ko/+)/Wnt1-Cre male mice with females homozygous for the Alk5flox (Alk2flox) allele and the R26R reporter[12,19].
T15919	30606-30610	NN	denotes	Alk5
T15921	30611-30612	-LRB-	denotes	(
T15922	30612-30615	CC	denotes	and
T15923	30616-30620	NN	denotes	Alk2
T15924	30620-30621	-RRB-	denotes	)
T15920	30622-30628	NN	denotes	mutant
T15926	30629-30632	CC	denotes	and
T15927	30633-30640	NN	denotes	control
T15925	30641-30648	NNS	denotes	embryos
T15929	30649-30653	VBD	denotes	were
T15928	30654-30663	VBN	denotes	generated
T15930	30664-30666	IN	denotes	by
T15931	30667-30673	VBG	denotes	mating
T15932	30674-30680	NN	denotes	Alk5ko
T15934	30680-30681	HYPH	denotes	/
T15935	30681-30682	SYM	denotes	+
T15936	30682-30683	-LRB-	denotes	(
T15937	30683-30689	NN	denotes	Alk2ko
T15938	30689-30690	HYPH	denotes	/
T15939	30690-30691	SYM	denotes	+
T15940	30691-30692	-RRB-	denotes	)
T15941	30692-30693	HYPH	denotes	/
T15942	30693-30697	NN	denotes	Wnt1
T15944	30697-30698	HYPH	denotes	-
T15943	30698-30701	NN	denotes	Cre
T15945	30702-30706	JJ	denotes	male
T15933	30707-30711	NNS	denotes	mice
T15946	30712-30716	IN	denotes	with
T15947	30717-30724	NNS	denotes	females
T15948	30725-30735	JJ	denotes	homozygous
T15949	30736-30739	IN	denotes	for
T15950	30740-30743	DT	denotes	the
T15952	30744-30752	NN	denotes	Alk5flox
T15953	30753-30754	-LRB-	denotes	(
T15954	30754-30762	NN	denotes	Alk2flox
T15955	30762-30763	-RRB-	denotes	)
T15951	30764-30770	NN	denotes	allele
T15956	30771-30774	CC	denotes	and
T15957	30775-30778	DT	denotes	the
T15959	30779-30783	NN	denotes	R26R
T15958	30784-30792	NN	denotes	reporter
T15960	30792-30793	-LRB-	denotes	[
T15962	30793-30795	CD	denotes	12
T15963	30795-30796	,	denotes	,
T15961	30796-30798	CD	denotes	19
T15964	30798-30799	-RRB-	denotes	]
T15965	30799-30800	.	denotes	.
T15966	30800-30862	sentence	denotes	Genotyping was performed by PCR as described earlier [21,42].
T15967	30801-30811	NN	denotes	Genotyping
T15969	30812-30815	VBD	denotes	was
T15968	30816-30825	VBN	denotes	performed
T15970	30826-30828	IN	denotes	by
T15971	30829-30832	NN	denotes	PCR
T15972	30833-30835	IN	denotes	as
T15973	30836-30845	VBN	denotes	described
T15974	30846-30853	RBR	denotes	earlier
T15975	30854-30855	-LRB-	denotes	[
T15977	30855-30857	CD	denotes	21
T15978	30857-30858	,	denotes	,
T15976	30858-30860	CD	denotes	42
T15979	30860-30861	-RRB-	denotes	]
T15980	30861-30862	.	denotes	.
T15981	30862-31000	sentence	denotes	Wnt1-Cre mice were kindly provided by A. McMahon (Harvard University) and R26R reporter mice were obtained from the Jackson laboratories.
T15982	30863-30867	NN	denotes	Wnt1
T15984	30867-30868	HYPH	denotes	-
T15983	30868-30871	NN	denotes	Cre
T15985	30872-30876	NNS	denotes	mice
T15987	30877-30881	VBD	denotes	were
T15988	30882-30888	RB	denotes	kindly
T15986	30889-30897	VBN	denotes	provided
T15989	30898-30900	IN	denotes	by
T15990	30901-30903	NNP	denotes	A.
T15991	30904-30911	NNP	denotes	McMahon
T15992	30912-30913	-LRB-	denotes	(
T15994	30913-30920	NNP	denotes	Harvard
T15993	30921-30931	NNP	denotes	University
T15995	30931-30932	-RRB-	denotes	)
T15996	30933-30936	CC	denotes	and
T15997	30937-30941	NN	denotes	R26R
T15998	30942-30950	NN	denotes	reporter
T15999	30951-30955	NNS	denotes	mice
T16001	30956-30960	VBD	denotes	were
T16000	30961-30969	VBN	denotes	obtained
T16002	30970-30974	IN	denotes	from
T16003	30975-30978	DT	denotes	the
T16005	30979-30986	NNP	denotes	Jackson
T16004	30987-30999	NNS	denotes	laboratories
T16006	30999-31000	.	denotes	.
T16007	31000-31168	sentence	denotes	All studies were carried out at the Animal Care Facility of the Saban Research Institute of Childrens Hospital Los Angeles in accordance with institutional guidelines.
T16008	31001-31004	DT	denotes	All
T16009	31005-31012	NNS	denotes	studies
T16011	31013-31017	VBD	denotes	were
T16010	31018-31025	VBN	denotes	carried
T16012	31026-31029	RP	denotes	out
T16013	31030-31032	IN	denotes	at
T16014	31033-31036	DT	denotes	the
T16016	31037-31043	NN	denotes	Animal
T16017	31044-31048	NN	denotes	Care
T16015	31049-31057	NN	denotes	Facility
T16018	31058-31060	IN	denotes	of
T16019	31061-31064	DT	denotes	the
T16021	31065-31070	NNP	denotes	Saban
T16022	31071-31079	NNP	denotes	Research
T16020	31080-31089	NNP	denotes	Institute
T16023	31090-31092	IN	denotes	of
T16024	31093-31102	NNP	denotes	Childrens
T16025	31103-31111	NNP	denotes	Hospital
T16026	31112-31115	NNP	denotes	Los
T16027	31116-31123	NNP	denotes	Angeles
T16028	31124-31126	IN	denotes	in
T16029	31127-31137	NN	denotes	accordance
T16030	31138-31142	IN	denotes	with
T16031	31143-31156	JJ	denotes	institutional
T16032	31157-31167	NNS	denotes	guidelines
T16033	31167-31168	.	denotes	.
T16142	31170-31175	VBN	denotes	Timed
T16143	31176-31184	NNS	denotes	mataings
T16144	31184-31312	sentence	denotes	Mice were mated during the dark period of the controlled light cycle; presence of vaginal plugs was designated as day 0 hour 0.
T16145	31185-31189	NNS	denotes	Mice
T16147	31190-31194	VBD	denotes	were
T16146	31195-31200	VBN	denotes	mated
T16149	31201-31207	IN	denotes	during
T16150	31208-31211	DT	denotes	the
T16152	31212-31216	JJ	denotes	dark
T16151	31217-31223	NN	denotes	period
T16153	31224-31226	IN	denotes	of
T16154	31227-31230	DT	denotes	the
T16156	31231-31241	VBN	denotes	controlled
T16157	31242-31247	NN	denotes	light
T16155	31248-31253	NN	denotes	cycle
T16158	31253-31254	:	denotes	;
T16159	31255-31263	NN	denotes	presence
T16160	31264-31266	IN	denotes	of
T16161	31267-31274	JJ	denotes	vaginal
T16162	31275-31280	NNS	denotes	plugs
T16163	31281-31284	VBD	denotes	was
T16148	31285-31295	VBN	denotes	designated
T16164	31296-31298	IN	denotes	as
T16165	31299-31302	NN	denotes	day
T16166	31303-31304	CD	denotes	0
T16167	31305-31309	NN	denotes	hour
T16168	31310-31311	CD	denotes	0
T16169	31311-31312	.	denotes	.
T16170	31312-31412	sentence	denotes	Females were euthanized by CO2, and embryos were collected in Hanks' balanced salt solution on ice.
T16171	31313-31320	NNS	denotes	Females
T16173	31321-31325	VBD	denotes	were
T16172	31326-31336	VBN	denotes	euthanized
T16174	31337-31339	IN	denotes	by
T16175	31340-31343	NN	denotes	CO2
T16176	31343-31345	,	denotes	,
T16177	31345-31348	CC	denotes	and
T16178	31349-31356	NNS	denotes	embryos
T16180	31357-31361	VBD	denotes	were
T16179	31362-31371	VBN	denotes	collected
T16181	31372-31374	IN	denotes	in
T16182	31375-31380	NNP	denotes	Hanks
T16184	31380-31381	POS	denotes	'
T16185	31382-31390	VBN	denotes	balanced
T16186	31391-31395	NN	denotes	salt
T16183	31396-31404	NN	denotes	solution
T16187	31405-31407	IN	denotes	on
T16188	31408-31411	NN	denotes	ice
T16189	31411-31412	.	denotes	.
T16575	31414-31426	JJ	denotes	Histological
T16576	31427-31435	NNS	denotes	analyses
T16577	31435-31540	sentence	denotes	Embryos (E17) were fixed with 4% paraformaldehyhe for 14 hours, dehydrated and embedded in paraffin wax.
T16578	31436-31443	NNS	denotes	Embryos
T16580	31444-31445	-LRB-	denotes	(
T16581	31445-31448	NN	denotes	E17
T16582	31448-31449	-RRB-	denotes	)
T16583	31450-31454	VBD	denotes	were
T16579	31455-31460	VBN	denotes	fixed
T16584	31461-31465	IN	denotes	with
T16585	31466-31467	CD	denotes	4
T16586	31467-31468	NN	denotes	%
T16587	31469-31485	NN	denotes	paraformaldehyhe
T16588	31486-31489	IN	denotes	for
T16589	31490-31492	CD	denotes	14
T16590	31493-31498	NNS	denotes	hours
T16591	31498-31500	,	denotes	,
T16592	31500-31510	VBN	denotes	dehydrated
T16593	31511-31514	CC	denotes	and
T16594	31515-31523	VBN	denotes	embedded
T16595	31524-31526	IN	denotes	in
T16596	31527-31535	NN	denotes	paraffin
T16597	31536-31539	NN	denotes	wax
T16598	31539-31540	.	denotes	.
T16599	31540-31623	sentence	denotes	Sections (7–8 um) were stained with Hematoxylin and Eosin (n≥3 for each genotype).
T16600	31541-31549	NNS	denotes	Sections
T16602	31550-31551	-LRB-	denotes	(
T16604	31551-31552	CD	denotes	7
T16606	31552-31553	SYM	denotes	–
T16605	31553-31554	CD	denotes	8
T16603	31555-31557	NN	denotes	um
T16607	31557-31558	-RRB-	denotes	)
T16608	31559-31563	VBD	denotes	were
T16601	31564-31571	VBN	denotes	stained
T16609	31572-31576	IN	denotes	with
T16610	31577-31588	NN	denotes	Hematoxylin
T16611	31589-31592	CC	denotes	and
T16612	31593-31598	NN	denotes	Eosin
T16613	31599-31600	-LRB-	denotes	(
T16615	31600-31601	NN	denotes	n
T16616	31601-31602	SYM	denotes	≥
T16614	31602-31603	CD	denotes	3
T16617	31604-31607	IN	denotes	for
T16618	31608-31612	DT	denotes	each
T16619	31613-31621	NN	denotes	genotype
T16620	31621-31622	-RRB-	denotes	)
T16621	31622-31623	.	denotes	.
T16622	31623-31722	sentence	denotes	For lineage tracing analyses, embryos were stained for β-galactosidase activity as described [43].
T16623	31624-31627	IN	denotes	For
T16625	31628-31635	NN	denotes	lineage
T16626	31636-31643	NN	denotes	tracing
T16627	31644-31652	NNS	denotes	analyses
T16628	31652-31654	,	denotes	,
T16629	31654-31661	NNS	denotes	embryos
T16630	31662-31666	VBD	denotes	were
T16624	31667-31674	VBN	denotes	stained
T16631	31675-31678	IN	denotes	for
T16632	31679-31680	NN	denotes	β
T16634	31680-31681	HYPH	denotes	-
T16633	31681-31694	NN	denotes	galactosidase
T16635	31695-31703	NN	denotes	activity
T16636	31704-31706	IN	denotes	as
T16637	31707-31716	VBN	denotes	described
T16638	31717-31718	-LRB-	denotes	[
T16639	31718-31720	CD	denotes	43
T16640	31720-31721	-RRB-	denotes	]
T16641	31721-31722	.	denotes	.
T16642	31722-31955	sentence	denotes	Briefly, the specimens (E11.0 – E11.5) were fixed in 4% paraformaldehyde for 30 minutes at room temperature, washed 3 times for 10 minutes in the detergent wash and developed for 2–12 hours in X-gal solution (n≥3 for each genotype).
T16643	31723-31730	RB	denotes	Briefly
T16645	31730-31732	,	denotes	,
T16646	31732-31735	DT	denotes	the
T16647	31736-31745	NNS	denotes	specimens
T16648	31746-31747	-LRB-	denotes	(
T16649	31747-31752	NN	denotes	E11.0
T16650	31753-31754	SYM	denotes	–
T16651	31755-31760	NN	denotes	E11.5
T16652	31760-31761	-RRB-	denotes	)
T16653	31762-31766	VBD	denotes	were
T16644	31767-31772	VBN	denotes	fixed
T16654	31773-31775	IN	denotes	in
T16655	31776-31777	CD	denotes	4
T16656	31777-31778	NN	denotes	%
T16657	31779-31795	NN	denotes	paraformaldehyde
T16658	31796-31799	IN	denotes	for
T16659	31800-31802	CD	denotes	30
T16660	31803-31810	NNS	denotes	minutes
T16661	31811-31813	IN	denotes	at
T16662	31814-31818	NN	denotes	room
T16663	31819-31830	NN	denotes	temperature
T16664	31830-31832	,	denotes	,
T16665	31832-31838	VBN	denotes	washed
T16666	31839-31840	CD	denotes	3
T16667	31841-31846	NNS	denotes	times
T16668	31847-31850	IN	denotes	for
T16669	31851-31853	CD	denotes	10
T16670	31854-31861	NNS	denotes	minutes
T16671	31862-31864	IN	denotes	in
T16672	31865-31868	DT	denotes	the
T16674	31869-31878	NN	denotes	detergent
T16673	31879-31883	NN	denotes	wash
T16675	31884-31887	CC	denotes	and
T16676	31888-31897	VBN	denotes	developed
T16677	31898-31901	IN	denotes	for
T16678	31902-31903	CD	denotes	2
T16680	31903-31904	SYM	denotes	–
T16679	31904-31906	CD	denotes	12
T16681	31907-31912	NNS	denotes	hours
T16682	31913-31915	IN	denotes	in
T16683	31916-31917	NN	denotes	X
T16685	31917-31918	HYPH	denotes	-
T16684	31918-31921	NN	denotes	gal
T16686	31922-31930	NN	denotes	solution
T16687	31931-31932	-LRB-	denotes	(
T16689	31932-31933	NN	denotes	n
T16690	31933-31934	SYM	denotes	≥
T16688	31934-31935	CD	denotes	3
T16691	31936-31939	IN	denotes	for
T16692	31940-31944	DT	denotes	each
T16693	31945-31953	NN	denotes	genotype
T16694	31953-31954	-RRB-	denotes	)
T16695	31954-31955	.	denotes	.
T16696	31955-32179	sentence	denotes	For immunohistochemistry, fixed sections from tissues harvested at E10.5 – E11.5 were stained with monoclonal α-smooth muscle actin (DAKO), cleaved caspase-3 (Cell Signaling) or phophohistone-H3 (Cell Signaling) antibodies.
T16697	31956-31959	IN	denotes	For
T16699	31960-31980	NN	denotes	immunohistochemistry
T16700	31980-31982	,	denotes	,
T16701	31982-31987	VBN	denotes	fixed
T16702	31988-31996	NNS	denotes	sections
T16703	31997-32001	IN	denotes	from
T16704	32002-32009	NNS	denotes	tissues
T16705	32010-32019	VBN	denotes	harvested
T16706	32020-32022	IN	denotes	at
T16707	32023-32028	NN	denotes	E10.5
T16708	32029-32030	SYM	denotes	–
T16709	32031-32036	NN	denotes	E11.5
T16710	32037-32041	VBD	denotes	were
T16698	32042-32049	VBN	denotes	stained
T16711	32050-32054	IN	denotes	with
T16712	32055-32065	JJ	denotes	monoclonal
T16714	32066-32067	NN	denotes	α
T16716	32067-32068	HYPH	denotes	-
T16717	32068-32074	JJ	denotes	smooth
T16715	32075-32081	NN	denotes	muscle
T16713	32082-32087	NN	denotes	actin
T16719	32088-32089	-LRB-	denotes	(
T16720	32089-32093	NN	denotes	DAKO
T16721	32093-32094	-RRB-	denotes	)
T16722	32094-32096	,	denotes	,
T16723	32096-32103	VBN	denotes	cleaved
T16724	32104-32111	NN	denotes	caspase
T16725	32111-32112	HYPH	denotes	-
T16726	32112-32113	CD	denotes	3
T16727	32114-32115	-LRB-	denotes	(
T16729	32115-32119	NNP	denotes	Cell
T16728	32120-32129	NNP	denotes	Signaling
T16730	32129-32130	-RRB-	denotes	)
T16731	32131-32133	CC	denotes	or
T16732	32134-32147	NN	denotes	phophohistone
T16734	32147-32148	HYPH	denotes	-
T16733	32148-32150	NN	denotes	H3
T16735	32151-32152	-LRB-	denotes	(
T16737	32152-32156	NNP	denotes	Cell
T16736	32157-32166	NNP	denotes	Signaling
T16738	32166-32167	-RRB-	denotes	)
T16718	32168-32178	NNS	denotes	antibodies
T16739	32178-32179	.	denotes	.
T16740	32179-32262	sentence	denotes	TUNEL assays were performed using the DeadEnd fluorometric TUNEL system (Promega).
T16741	32180-32185	NN	denotes	TUNEL
T16742	32186-32192	NNS	denotes	assays
T16744	32193-32197	VBD	denotes	were
T16743	32198-32207	VBN	denotes	performed
T16745	32208-32213	VBG	denotes	using
T16746	32214-32217	DT	denotes	the
T16748	32218-32225	NNP	denotes	DeadEnd
T16750	32226-32238	JJ	denotes	fluorometric
T16749	32239-32244	NN	denotes	TUNEL
T16747	32245-32251	NN	denotes	system
T16751	32252-32253	-LRB-	denotes	(
T16752	32253-32260	NNP	denotes	Promega
T16753	32260-32261	-RRB-	denotes	)
T16754	32261-32262	.	denotes	.
T16755	32262-32327	sentence	denotes	In each assay 3 or more embryos were analyzed for each genotype.
T16756	32263-32265	IN	denotes	In
T16758	32266-32270	DT	denotes	each
T16759	32271-32276	NN	denotes	assay
T16760	32277-32278	CD	denotes	3
T16762	32279-32281	CC	denotes	or
T16763	32282-32286	JJR	denotes	more
T16761	32287-32294	NNS	denotes	embryos
T16764	32295-32299	VBD	denotes	were
T16757	32300-32308	VBN	denotes	analyzed
T16765	32309-32312	IN	denotes	for
T16766	32313-32317	DT	denotes	each
T16767	32318-32326	NN	denotes	genotype
T16768	32326-32327	.	denotes	.
T16958	32329-32332	NN	denotes	Ink
T16960	32333-32336	CC	denotes	and
T16961	32337-32344	NN	denotes	casting
T16962	32345-32348	NN	denotes	dye
T16959	32349-32359	NNS	denotes	injections
T16963	32359-32464	sentence	denotes	Embryos (E10.0 – E13.0) were dissected and placed in ice cold PBS (n≥3 per genotype in each time point).
T16964	32360-32367	NNS	denotes	Embryos
T16966	32368-32369	-LRB-	denotes	(
T16967	32369-32374	NN	denotes	E10.0
T16968	32375-32376	SYM	denotes	–
T16969	32377-32382	NN	denotes	E13.0
T16970	32382-32383	-RRB-	denotes	)
T16971	32384-32388	VBD	denotes	were
T16965	32389-32398	VBN	denotes	dissected
T16972	32399-32402	CC	denotes	and
T16973	32403-32409	VBN	denotes	placed
T16974	32410-32412	IN	denotes	in
T16975	32413-32416	NN	denotes	ice
T16977	32417-32421	JJ	denotes	cold
T16976	32422-32425	NN	denotes	PBS
T16978	32426-32427	-LRB-	denotes	(
T16980	32427-32428	NN	denotes	n
T16981	32428-32429	SYM	denotes	≥
T16979	32429-32430	CD	denotes	3
T16982	32431-32434	IN	denotes	per
T16983	32435-32443	NN	denotes	genotype
T16984	32444-32446	IN	denotes	in
T16985	32447-32451	DT	denotes	each
T16987	32452-32456	NN	denotes	time
T16986	32457-32462	NN	denotes	point
T16988	32462-32463	-RRB-	denotes	)
T16989	32463-32464	.	denotes	.
T16990	32464-32638	sentence	denotes	Using a pulled glass pipette, India ink or Yellow casting dye (Connecticut Valley Biological Supply) was injected into the ventricles until ink/dye penetrated small vessels.
T16991	32465-32470	VBG	denotes	Using
T16993	32471-32472	DT	denotes	a
T16995	32473-32479	VBN	denotes	pulled
T16996	32480-32485	NN	denotes	glass
T16994	32486-32493	NN	denotes	pipette
T16997	32493-32495	,	denotes	,
T16998	32495-32500	NNP	denotes	India
T16999	32501-32504	NN	denotes	ink
T17000	32505-32507	CC	denotes	or
T17001	32508-32514	JJ	denotes	Yellow
T17003	32515-32522	NN	denotes	casting
T17002	32523-32526	NN	denotes	dye
T17004	32527-32528	-LRB-	denotes	(
T17006	32528-32539	NNP	denotes	Connecticut
T17007	32540-32546	NNP	denotes	Valley
T17008	32547-32557	NNP	denotes	Biological
T17005	32558-32564	NNP	denotes	Supply
T17009	32564-32565	-RRB-	denotes	)
T17010	32566-32569	VBD	denotes	was
T16992	32570-32578	VBN	denotes	injected
T17011	32579-32583	IN	denotes	into
T17012	32584-32587	DT	denotes	the
T17013	32588-32598	NNS	denotes	ventricles
T17014	32599-32604	IN	denotes	until
T17016	32605-32608	NN	denotes	ink
T17018	32608-32609	HYPH	denotes	/
T17017	32609-32612	NN	denotes	dye
T17015	32613-32623	VBD	denotes	penetrated
T17019	32624-32629	JJ	denotes	small
T17020	32630-32637	NNS	denotes	vessels
T17021	32637-32638	.	denotes	.
T17022	32638-32767	sentence	denotes	Embryos were postfixed in 10% buffered formalin for 12 hours, dehydrated and cleared in benzylbenzoate: benzyl alcohol (2:1v/v).
T17023	32639-32646	NNS	denotes	Embryos
T17025	32647-32651	VBD	denotes	were
T17024	32652-32661	VBN	denotes	postfixed
T17026	32662-32664	IN	denotes	in
T17027	32665-32667	CD	denotes	10
T17028	32667-32668	NN	denotes	%
T17030	32669-32677	VBN	denotes	buffered
T17029	32678-32686	NN	denotes	formalin
T17031	32687-32690	IN	denotes	for
T17032	32691-32693	CD	denotes	12
T17033	32694-32699	NNS	denotes	hours
T17034	32699-32701	,	denotes	,
T17035	32701-32711	VBN	denotes	dehydrated
T17036	32712-32715	CC	denotes	and
T17037	32716-32723	VBN	denotes	cleared
T17038	32724-32726	IN	denotes	in
T17039	32727-32741	NN	denotes	benzylbenzoate
T17040	32741-32743	:	denotes	:
T17041	32743-32749	NN	denotes	benzyl
T17042	32750-32757	NN	denotes	alcohol
T17043	32758-32759	-LRB-	denotes	(
T17044	32759-32760	CD	denotes	2
T17045	32760-32761	SYM	denotes	:
T17047	32761-32763	NN	denotes	1v
T17048	32763-32764	HYPH	denotes	/
T17046	32764-32765	NN	denotes	v
T17049	32765-32766	-RRB-	denotes	)
T17050	32766-32767	.	denotes	.
T17140	32769-32779	NN	denotes	Expression
T17141	32780-32788	NNS	denotes	analyses
T17142	32788-32974	sentence	denotes	To visualize parathyroid hormone expression we used in situ hybridization and an antisense probe corresponding to nucleotides 97–534 (kindly provided by Nancy Manley) as described [44].
T17143	32789-32791	TO	denotes	To
T17144	32792-32801	VB	denotes	visualize
T17146	32802-32813	NN	denotes	parathyroid
T17147	32814-32821	NN	denotes	hormone
T17148	32822-32832	NN	denotes	expression
T17149	32833-32835	PRP	denotes	we
T17145	32836-32840	VBD	denotes	used
T17150	32841-32843	FW	denotes	in
T17151	32844-32848	FW	denotes	situ
T17152	32849-32862	NN	denotes	hybridization
T17153	32863-32866	CC	denotes	and
T17154	32867-32869	DT	denotes	an
T17156	32870-32879	NN	denotes	antisense
T17155	32880-32885	NN	denotes	probe
T17157	32886-32899	VBG	denotes	corresponding
T17158	32900-32902	IN	denotes	to
T17159	32903-32914	NNS	denotes	nucleotides
T17160	32915-32917	CD	denotes	97
T17162	32917-32918	SYM	denotes	–
T17161	32918-32921	CD	denotes	534
T17163	32922-32923	-LRB-	denotes	(
T17164	32923-32929	RB	denotes	kindly
T17165	32930-32938	VBN	denotes	provided
T17166	32939-32941	IN	denotes	by
T17167	32942-32947	NNP	denotes	Nancy
T17168	32948-32954	NNP	denotes	Manley
T17169	32954-32955	-RRB-	denotes	)
T17170	32956-32958	IN	denotes	as
T17171	32959-32968	VBN	denotes	described
T17172	32969-32970	-LRB-	denotes	[
T17173	32970-32972	CD	denotes	44
T17174	32972-32973	-RRB-	denotes	]
T17175	32973-32974	.	denotes	.
R100	T612	T613	nsubj	We,deleted
R1000	T3489	T3486	nsubj	embryos,display
R1001	T3490	T3489	compound	mutant,embryos
R1002	T3491	T3492	amod	severe,sac
R1003	T3492	T3494	nmod	sac,defects
R1004	T3493	T3492	amod	aortic,sac
R1005	T3494	T3486	dobj	defects,display
R1006	T3495	T3492	cc	and,sac
R1007	T3496	T3497	amod	pharyngeal,artery
R1008	T3497	T3492	conj	artery,sac
R1009	T3498	T3497	compound	arch,artery
R101	T614	T615	det	the,Alk5
R1010	T3499	T3486	punct	", ",display
R1011	T3500	T3486	cc	and,display
R1012	T3501	T3486	conj	failed,display
R1013	T3502	T3503	amod	aortico,pulmonary
R1014	T3503	T3505	amod	pulmonary,septation
R1015	T3504	T3503	punct	-,pulmonary
R1016	T3505	T3501	dobj	septation,failed
R1017	T3506	T3505	acl	leading,septation
R1018	T3507	T3506	prep	to,leading
R1019	T3508	T3507	pobj	PTA,to
R102	T615	T613	dobj	Alk5,deleted
R1020	T3509	T3486	punct	.,display
R1021	T3511	T3512	poss	Our,data
R1022	T3512	T3513	nsubj	data,suggest
R1023	T3514	T3513	advmod	further,suggest
R1024	T3515	T3516	mark	that,result
R1025	T3516	T3513	ccomp	result,suggest
R1026	T3517	T3518	advmod	at,least
R1027	T3518	T3519	advmod	least,some
R1028	T3519	T3516	nsubj	some,result
R1029	T3520	T3519	prep	of,some
R103	T616	T617	nmod	TGF,β
R1030	T3521	T3522	det	these,phenotypes
R1031	T3522	T3520	pobj	phenotypes,of
R1032	T3523	T3522	amod	abnormal,phenotypes
R1033	T3524	T3522	amod	detected,phenotypes
R1034	T3525	T3516	prep	from,result
R1035	T3526	T3527	det	a,increase
R1036	T3527	T3525	pobj	increase,from
R1037	T3528	T3527	amod	dramatic,increase
R1038	T3529	T3527	prep	in,increase
R1039	T3530	T3529	pobj	apoptosis,in
R104	T617	T615	nmod	β,Alk5
R1040	T3531	T3530	prep	of,apoptosis
R1041	T3532	T3533	amod	postmigratory,cells
R1042	T3533	T3531	pobj	cells,of
R1043	T3534	T3535	amod	cardiac,crest
R1044	T3535	T3533	compound	crest,cells
R1045	T3536	T3535	amod	neural,crest
R1046	T3537	T3513	punct	.,suggest
R1047	T3539	T3540	det	These,phenotypes
R1048	T3540	T3541	nsubj	phenotypes,differ
R1049	T3542	T3541	advmod	remarkably,differ
R105	T618	T617	punct	-,β
R1050	T3543	T3541	prep	from,differ
R1051	T3544	T3543	pobj	those,from
R1052	T3545	T3544	acl	seen,those
R1053	T3546	T3545	prep	in,seen
R1054	T3547	T3548	amod	corresponding,mutants
R1055	T3548	T3546	pobj	mutants,in
R1056	T3549	T3548	compound	Tgfbr2,mutants
R1057	T3550	T3545	punct	", ",seen
R1058	T3551	T3545	advcl	suggesting,seen
R1059	T3552	T3553	mark	that,mediates
R106	T619	T615	nmod	type,Alk5
R1060	T3553	T3551	ccomp	mediates,suggesting
R1061	T3554	T3553	nsubj	ALK5,mediates
R1062	T3555	T3556	det	a,spectrum
R1063	T3556	T3553	dobj	spectrum,mediates
R1064	T3557	T3556	amod	wider,spectrum
R1065	T3558	T3556	prep	of,spectrum
R1066	T3559	T3560	compound	signaling,events
R1067	T3560	T3558	pobj	events,of
R1068	T3561	T3556	prep	than,spectrum
R1069	T3562	T3563	poss	its,partner
R107	T620	T619	nummod	I,type
R1070	T3563	T3561	pobj	partner,than
R1071	T3564	T3563	amod	classical,partner
R1072	T3565	T3563	compound	binding,partner
R1073	T3566	T3563	appos	TGFβRII,partner
R1074	T3567	T3553	prep	in,mediates
R1075	T3568	T3569	amod	cardiac,cells
R1076	T3569	T3567	pobj	cells,in
R1077	T3570	T3569	amod	neural,cells
R1078	T3571	T3569	compound	crest,cells
R1079	T3572	T3553	prep	during,mediates
R108	T621	T615	compound	receptor,Alk5
R1080	T3573	T3574	amod	cardiac,development
R1081	T3574	T3572	pobj	development,during
R1082	T3575	T3573	cc	and,cardiac
R1083	T3576	T3573	conj	pharyngeal,cardiac
R109	T622	T613	advmod	specifically,deleted
R1094	T4627	T4628	amod	Persistent,arteriosus
R1095	T4629	T4628	compound	truncus,arteriosus
R1096	T4630	T4628	cc	and,arteriosus
R1097	T4631	T4632	amod	abnormal,vessels
R1098	T4632	T4628	conj	vessels,arteriosus
R1099	T4633	T4632	amod	large,vessels
R110	T623	T613	prep	in,deleted
R1100	T4634	T4628	prep	in,arteriosus
R1101	T4635	T4634	pobj	mice,in
R1102	T4636	T4635	acl	lacking,mice
R1103	T4637	T4636	dobj	Alk5,lacking
R1104	T4638	T4636	prep	in,lacking
R1105	T4639	T4640	amod	cardiac,NCCs
R1106	T4640	T4638	pobj	NCCs,in
R1107	T4642	T4643	aux	To,inactivate
R1108	T4643	T4644	advcl	inactivate,crossed
R1109	T4645	T4643	dobj	Alk5,inactivate
R111	T624	T625	det	the,lineage
R1110	T4646	T4643	prep	in,inactivate
R1111	T4647	T4648	amod	cardiac,NCCs
R1112	T4648	T4646	pobj	NCCs,in
R1113	T4649	T4644	punct	", ",crossed
R1114	T4650	T4644	nsubjpass	mice,crossed
R1115	T4651	T4650	amod	homozygous,mice
R1116	T4652	T4651	prep	for,homozygous
R1117	T4653	T4654	det	the,allele
R1118	T4654	T4652	pobj	allele,for
R1119	T4655	T4654	amod	floxed,allele
R112	T625	T623	pobj	lineage,in
R1120	T4656	T4654	compound	Alk5,allele
R1121	T4657	T4654	punct	(,allele
R1122	T4658	T4659	compound	Alk5Flox,Flox
R1123	T4659	T4654	appos	Flox,allele
R1124	T4660	T4659	punct	/,Flox
R1125	T4661	T4650	punct	),mice
R1126	T4662	T4663	punct	[,21
R1127	T4663	T4650	parataxis	21,mice
R1128	T4664	T4663	punct	],21
R1129	T4665	T4644	auxpass	were,crossed
R113	T626	T625	nmod	mouse,lineage
R1130	T4666	T4644	prep	with,crossed
R1131	T4667	T4668	amod	transgenic,mice
R1132	T4668	T4666	pobj	mice,with
R1133	T4669	T4670	compound	Wnt1,Cre
R1134	T4670	T4668	compound	Cre,mice
R1135	T4671	T4670	punct	-,Cre
R1136	T4672	T4673	punct	[,22
R1137	T4673	T4668	parataxis	22,mice
R1138	T4674	T4673	punct	],22
R1139	T4675	T4668	punct	", ",mice
R114	T627	T628	amod	neural,cell
R1140	T4676	T4677	dep	which,were
R1141	T4677	T4668	relcl	were,mice
R1142	T4678	T4677	advmod	also,were
R1143	T4679	T4677	acomp	heterozygous,were
R1144	T4680	T4679	prep	for,heterozygous
R1145	T4681	T4682	det	the,allele
R1146	T4682	T4680	pobj	allele,for
R1147	T4683	T4682	compound	Alk5,allele
R1148	T4684	T4682	compound	knockout,allele
R1149	T4685	T4682	punct	(,allele
R115	T628	T625	compound	cell,lineage
R1150	T4686	T4687	nmod	Alk5KO,allele
R1151	T4687	T4682	appos	allele,allele
R1152	T4688	T4687	punct	),allele
R1153	T4689	T4644	punct	.,crossed
R1154	T4691	T4692	det	The,mice
R1155	T4692	T4694	nsubj	mice,had
R1156	T4693	T4692	amod	resulting,mice
R1157	T4695	T4692	amod	heterozygous,mice
R1158	T4696	T4695	prep	for,heterozygous
R1159	T4697	T4698	det	the,alleles
R116	T629	T628	compound	crest,cell
R1160	T4698	T4696	pobj	alleles,for
R1161	T4699	T4698	nmod	Alk5Flox,alleles
R1162	T4700	T4699	cc	and,Alk5Flox
R1163	T4701	T4699	conj	Alk5KO,Alk5Flox
R1164	T4702	T4698	punct	", ",alleles
R1165	T4703	T4704	dep	which,carried
R1166	T4704	T4698	relcl	carried,alleles
R1167	T4705	T4704	advmod	also,carried
R1168	T4706	T4707	det	the,transgene
R1169	T4707	T4704	dobj	transgene,carried
R117	T630	T613	punct	.,deleted
R1170	T4708	T4709	compound	Wnt1,Cre
R1171	T4709	T4707	compound	Cre,transgene
R1172	T4710	T4709	punct	-,Cre
R1173	T4711	T4694	punct	", ",had
R1174	T4712	T4713	det	the,gene
R1175	T4713	T4694	dobj	gene,had
R1176	T4714	T4713	compound	Alk5,gene
R1177	T4715	T4716	advmod	specifically,inactivated
R1178	T4716	T4713	acl	inactivated,gene
R1179	T4717	T4716	prep	in,inactivated
R118	T632	T633	nsubj	Failure,leads
R1180	T4718	T4717	pobj	NCCs,in
R1181	T4719	T4718	punct	(,NCCs
R1182	T4720	T4721	advmod	herein,termed
R1183	T4721	T4718	acl	termed,NCCs
R1184	T4722	T4723	compound	Alk5,Cre
R1185	T4723	T4721	oprd	Cre,termed
R1186	T4724	T4723	punct	/,Cre
R1187	T4725	T4723	compound	Wnt1,Cre
R1188	T4726	T4723	punct	-,Cre
R1189	T4727	T4694	punct	),had
R119	T634	T632	prep	in,Failure
R1190	T4728	T4694	punct	", ",had
R1191	T4729	T4730	mark	while,were
R1192	T4730	T4694	advcl	were,had
R1193	T4731	T4732	det	the,littermates
R1194	T4732	T4730	nsubj	littermates,were
R1195	T4733	T4732	prep	with,littermates
R1196	T4734	T4735	amod	remaining,combinations
R1197	T4735	T4733	pobj	combinations,with
R1198	T4736	T4735	amod	allelic,combinations
R1199	T4737	T4738	advmod	phenotypically,normal
R120	T635	T634	pobj	signaling,in
R1200	T4738	T4730	acomp	normal,were
R1201	T4739	T4730	cc	and,were
R1202	T4740	T4730	conj	served,were
R1203	T4741	T4740	prep	as,served
R1204	T4742	T4741	pobj	controls,as
R1205	T4743	T4744	punct	(,Cre
R1206	T4744	T4740	parataxis	Cre,served
R1207	T4745	T4744	dep	Alk5Flox,Cre
R1208	T4746	T4745	punct	/,Alk5Flox
R1209	T4747	T4745	punct	+,Alk5Flox
R121	T636	T632	prep	via,Failure
R1210	T4748	T4744	punct	", ",Cre
R1211	T4749	T4744	dep	Alk5KO,Cre
R1212	T4750	T4749	punct	/,Alk5KO
R1213	T4751	T4749	punct	+,Alk5KO
R1214	T4752	T4744	punct	;,Cre
R1215	T4753	T4744	compound	Wnt1,Cre
R1216	T4754	T4744	punct	-,Cre
R1217	T4755	T4744	punct	),Cre
R1218	T4756	T4694	punct	.,had
R1219	T4758	T4759	advmod	When,harvested
R122	T637	T636	pobj	ALK5,via
R1220	T4759	T4762	advcl	harvested,recovered
R1221	T4760	T4759	nsubjpass	embryos,harvested
R1222	T4761	T4759	auxpass	were,harvested
R1223	T4763	T4759	prep	during,harvested
R1224	T4764	T4765	det	the,day
R1225	T4765	T4763	pobj	day,during
R1226	T4766	T4765	amod	last,day
R1227	T4767	T4765	prep	of,day
R1228	T4768	T4767	pobj	gestation,of
R1229	T4769	T4762	punct	", ",recovered
R123	T638	T633	prep	to,leads
R1230	T4770	T4771	det	an,number
R1231	T4771	T4762	nsubjpass	number,recovered
R1232	T4772	T4771	amod	expected,number
R1233	T4773	T4771	punct	(,number
R1234	T4774	T4775	nummod	25,%
R1235	T4775	T4771	appos	%,number
R1236	T4776	T4771	punct	),number
R1237	T4777	T4771	prep	of,number
R1238	T4778	T4779	compound	Alk5,Cre
R1239	T4779	T4783	compound	Cre,mutants
R124	T639	T640	amod	severe,defects
R1240	T4780	T4779	punct	/,Cre
R1241	T4781	T4779	compound	Wnt1,Cre
R1242	T4782	T4779	punct	-,Cre
R1243	T4783	T4777	pobj	mutants,of
R1244	T4784	T4762	auxpass	were,recovered
R1245	T4785	T4762	punct	.,recovered
R1246	T4787	T4788	advmod	However,died
R1247	T4789	T4788	punct	", ",died
R1248	T4790	T4791	det	all,offspring
R1249	T4791	T4788	nsubj	offspring,died
R125	T640	T638	pobj	defects,to
R1250	T4792	T4791	compound	mutant,offspring
R1251	T4793	T4794	preconj	either,during
R1252	T4794	T4788	prep	during,died
R1253	T4795	T4796	det	the,birth
R1254	T4796	T4794	pobj	birth,during
R1255	T4797	T4794	cc	or,during
R1256	T4798	T4794	conj	during,during
R1257	T4799	T4800	det	the,hours
R1258	T4800	T4798	pobj	hours,during
R1259	T4801	T4800	amod	first,hours
R126	T641	T640	amod	cardiovascular,defects
R1260	T4802	T4800	amod	post-natal,hours
R1261	T4803	T4788	punct	.,died
R1262	T4805	T4806	aux	To,determine
R1263	T4806	T4807	advcl	determine,performed
R1264	T4808	T4806	punct	", ",determine
R1265	T4809	T4810	mark	if,had
R1266	T4810	T4806	ccomp	had,determine
R1267	T4811	T4812	npadvmod	ALK5,mediated
R1268	T4812	T4814	amod	mediated,signaling
R1269	T4813	T4812	punct	-,mediated
R127	T642	T641	cc	and,cardiovascular
R1270	T4814	T4810	nsubj	signaling,had
R1271	T4815	T4816	compound	TGF,β
R1272	T4816	T4814	compound	β,signaling
R1273	T4817	T4816	punct	-,β
R1274	T4818	T4814	punct	-,signaling
R1275	T4819	T4820	det	a,role
R1276	T4820	T4810	dobj	role,had
R1277	T4821	T4820	prep	in,role
R1278	T4822	T4821	pobj	development,in
R1279	T4823	T4822	prep	of,development
R128	T643	T641	conj	pharyngeal,cardiovascular
R1280	T4824	T4825	det	the,OFT
R1281	T4825	T4823	pobj	OFT,of
R1282	T4826	T4825	cc	and,OFT
R1283	T4827	T4828	amod	large,vessels
R1284	T4828	T4825	conj	vessels,OFT
R1285	T4829	T4828	prep	of,vessels
R1286	T4830	T4831	det	the,arch
R1287	T4831	T4829	pobj	arch,of
R1288	T4832	T4831	amod	aortic,arch
R1289	T4833	T4807	punct	", ",performed
R129	T644	T640	punct	", ",defects
R1290	T4834	T4807	nsubj	we,performed
R1291	T4835	T4836	amod	casting,dye
R1292	T4836	T4837	compound	dye,experiments
R1293	T4837	T4807	dobj	experiments,performed
R1294	T4838	T4807	prep	on,performed
R1295	T4839	T4840	compound	E17,embryos
R1296	T4840	T4838	pobj	embryos,on
R1297	T4841	T4842	punct	(,1A
R1298	T4842	T4807	parataxis	1A,performed
R1299	T4843	T4842	compound	Fig.,1A
R130	T645	T640	prep	including,defects
R1300	T4844	T4845	punct	–,D
R1301	T4845	T4842	prep	D,1A
R1302	T4846	T4842	punct	),1A
R1303	T4847	T4807	punct	.,performed
R1304	T4849	T4850	prep	In,separated
R1305	T4851	T4852	amod	wild,type
R1306	T4852	T4854	compound	type,embryos
R1307	T4853	T4852	punct	-,type
R1308	T4854	T4849	pobj	embryos,In
R1309	T4855	T4856	punct	(,1A
R131	T646	T647	amod	inappropriate,remodeling
R1310	T4856	T4854	parataxis	1A,embryos
R1311	T4857	T4856	compound	Fig.,1A
R1312	T4858	T4856	punct	),1A
R1313	T4859	T4850	punct	", ",separated
R1314	T4860	T4861	det	the,aorta
R1315	T4861	T4850	nsubjpass	aorta,separated
R1316	T4862	T4850	auxpass	was,separated
R1317	T4863	T4850	advmod	clearly,separated
R1318	T4864	T4850	prep	from,separated
R1319	T4865	T4866	det	the,trunk
R132	T647	T645	pobj	remodeling,including
R1320	T4866	T4864	pobj	trunk,from
R1321	T4867	T4866	amod	pulmonary,trunk
R1322	T4868	T4850	punct	", ",separated
R1323	T4869	T4850	cc	and,separated
R1324	T4870	T4871	det	the,arteries
R1325	T4871	T4880	nsubj	arteries,branched
R1326	T4872	T4873	amod	right,brachiocephalic
R1327	T4873	T4871	nmod	brachiocephalic,arteries
R1328	T4874	T4873	punct	", ",brachiocephalic
R1329	T4875	T4876	amod	left,carotid
R133	T648	T647	prep	of,remodeling
R1330	T4876	T4873	conj	carotid,brachiocephalic
R1331	T4877	T4876	cc	and,carotid
R1332	T4878	T4879	amod	left,subclavian
R1333	T4879	T4876	conj	subclavian,carotid
R1334	T4880	T4850	conj	branched,separated
R1335	T4881	T4882	advmod	directly,off
R1336	T4882	T4880	prep	off,branched
R1337	T4883	T4884	det	the,arch
R1338	T4884	T4882	pobj	arch,off
R1339	T4885	T4884	amod	aortic,arch
R134	T649	T650	amod	pharyngeal,arteries
R1340	T4886	T4850	punct	.,separated
R1341	T4888	T4889	prep	In,displayed
R1342	T4890	T4888	pobj	contrast,In
R1343	T4891	T4889	punct	", ",displayed
R1344	T4892	T4893	compound	Alk5,Wnt1Cre
R1345	T4893	T4895	compound	Wnt1Cre,embryos
R1346	T4894	T4893	punct	/,Wnt1Cre
R1347	T4895	T4889	nsubj	embryos,displayed
R1348	T4896	T4895	compound	mutant,embryos
R1349	T4897	T4889	advmod	consistently,displayed
R135	T650	T648	pobj	arteries,of
R1350	T4898	T4899	det	a,trunk
R1351	T4899	T4889	dobj	trunk,displayed
R1352	T4900	T4899	amod	single,trunk
R1353	T4901	T4899	amod	prominent,trunk
R1354	T4902	T4899	amod	arterial,trunk
R1355	T4903	T4904	punct	(,1C
R1356	T4904	T4899	parataxis	1C,trunk
R1357	T4905	T4904	compound	Fig.,1C
R1358	T4906	T4907	punct	–,D
R1359	T4907	T4904	prep	D,1C
R136	T651	T650	compound	arch,arteries
R1360	T4908	T4904	punct	),1C
R1361	T4909	T4889	punct	", ",displayed
R1362	T4910	T4911	mark	while,displayed
R1363	T4911	T4889	advcl	displayed,displayed
R1364	T4912	T4913	amod	corresponding,embryos
R1365	T4913	T4911	nsubj	embryos,displayed
R1366	T4914	T4913	compound	Tgfbr2,embryos
R1367	T4915	T4913	compound	mutant,embryos
R1368	T4916	T4917	punct	(,1B
R1369	T4917	T4913	parataxis	1B,embryos
R137	T652	T647	punct	", ",remodeling
R1370	T4918	T4917	compound	Fig.,1B
R1371	T4919	T4917	punct	),1B
R1372	T4920	T4921	amod	interrupted,arch
R1373	T4921	T4911	dobj	arch,displayed
R1374	T4922	T4921	amod	aortic,arch
R1375	T4923	T4911	punct	", ",displayed
R1376	T4924	T4925	mark	as,reported
R1377	T4925	T4911	advcl	reported,displayed
R1378	T4926	T4925	advmod	earlier,reported
R1379	T4927	T4928	punct	[,8
R138	T653	T654	amod	abnormal,development
R1380	T4928	T4925	parataxis	8,reported
R1381	T4929	T4928	punct	],8
R1382	T4930	T4889	punct	.,displayed
R1383	T4932	T4933	advmod	Approximately,40
R1384	T4933	T4934	nummod	40,%
R1385	T4934	T4935	nsubj	%,had
R1386	T4936	T4934	prep	of,%
R1387	T4937	T4938	det	the,mutants
R1388	T4938	T4936	pobj	mutants,of
R1389	T4939	T4938	compound	Alk5,mutants
R139	T654	T647	appos	development,remodeling
R1390	T4940	T4941	det	a,tract
R1391	T4941	T4935	dobj	tract,had
R1392	T4942	T4943	amod	right,sided
R1393	T4943	T4941	amod	sided,tract
R1394	T4944	T4943	punct	-,sided
R1395	T4945	T4941	compound	outflow,tract
R1396	T4946	T4935	punct	", ",had
R1397	T4947	T4935	prep	with,had
R1398	T4948	T4949	det	the,arch
R1399	T4949	T4951	nsubj	arch,connecting
R140	T655	T656	amod	aortic,sac
R1400	T4950	T4949	amod	retroesophageal,arch
R1401	T4951	T4947	pcomp	connecting,with
R1402	T4952	T4951	prep	to,connecting
R1403	T4953	T4954	det	the,aorta
R1404	T4954	T4952	pobj	aorta,to
R1405	T4955	T4954	amod	descending,aorta
R1406	T4956	T4952	cc	and,to
R1407	T4957	T4952	conj	to,to
R1408	T4958	T4959	det	the,artery
R1409	T4959	T4957	pobj	artery,to
R141	T656	T654	compound	sac,development
R1410	T4960	T4959	amod	left,artery
R1411	T4961	T4959	amod	subclavian,artery
R1412	T4962	T4935	punct	.,had
R1413	T4964	T4965	det	The,arteries
R1414	T4965	T4967	nsubj	arteries,originated
R1415	T4966	T4965	amod	carotid,arteries
R1416	T4968	T4969	preconj	either,from
R1417	T4969	T4967	prep	from,originated
R1418	T4970	T4971	det	a,bud
R1419	T4971	T4969	pobj	bud,from
R142	T657	T647	punct	", ",remodeling
R1420	T4972	T4971	amod	common,bud
R1421	T4973	T4971	acl	located,bud
R1422	T4974	T4973	prep	in,located
R1423	T4975	T4976	det	the,side
R1424	T4976	T4974	pobj	side,in
R1425	T4977	T4976	amod	ventral,side
R1426	T4978	T4976	prep	of,side
R1427	T4979	T4980	det	the,arch
R1428	T4980	T4978	pobj	arch,of
R1429	T4981	T4980	amod	ascending,arch
R143	T658	T647	appos	failure,remodeling
R1430	T4982	T4969	punct	", ",from
R1431	T4983	T4969	cc	or,from
R1432	T4984	T4969	conj	from,from
R1433	T4985	T4986	amod	separate,sites
R1434	T4986	T4984	pobj	sites,from
R1435	T4987	T4986	amod	adjacent,sites
R1436	T4988	T4967	punct	", ",originated
R1437	T4989	T4990	mark	as,verified
R1438	T4990	T4967	advcl	verified,originated
R1439	T4991	T4990	prep	by,verified
R144	T659	T658	prep	in,failure
R1440	T4992	T4993	amod	serial,sectioning
R1441	T4993	T4991	pobj	sectioning,by
R1442	T4994	T4995	punct	(,1M
R1443	T4995	T4967	parataxis	1M,originated
R1444	T4996	T4995	compound	Fig.,1M
R1445	T4997	T4998	punct	–,P
R1446	T4998	T4995	prep	P,1M
R1447	T4999	T4995	punct	),1M
R1448	T5000	T4967	punct	.,originated
R1449	T5002	T5003	det	The,mutants
R145	T660	T661	amod	pharyngeal,migration
R1450	T5003	T5005	nsubj	mutants,displayed
R1451	T5004	T5003	amod	remaining,mutants
R1452	T5006	T5007	det	a,arch
R1453	T5007	T5005	dobj	arch,displayed
R1454	T5008	T5009	amod	left,sided
R1455	T5009	T5007	amod	sided,arch
R1456	T5010	T5009	punct	-,sided
R1457	T5011	T5007	amod	aortic,arch
R1458	T5012	T5007	punct	", ",arch
R1459	T5013	T5014	advmod	where,originated
R146	T661	T659	pobj	migration,in
R1460	T5014	T5007	relcl	originated,arch
R1461	T5015	T5016	det	the,arteries
R1462	T5016	T5014	nsubj	arteries,originated
R1463	T5017	T5016	amod	right,arteries
R1464	T5018	T5016	compound	carotid,arteries
R1465	T5019	T5014	prep	from,originated
R1466	T5020	T5021	det	the,aspect
R1467	T5021	T5019	pobj	aspect,from
R1468	T5022	T5021	amod	right,aspect
R1469	T5023	T5021	amod	lateral,aspect
R147	T662	T661	compound	organ,migration
R1470	T5024	T5021	prep	of,aspect
R1471	T5025	T5026	det	the,trunk
R1472	T5026	T5024	pobj	trunk,of
R1473	T5027	T5026	amod	ascending,trunk
R1474	T5028	T5005	punct	", ",displayed
R1475	T5029	T5030	mark	while,budded
R1476	T5030	T5005	advcl	budded,displayed
R1477	T5031	T5032	det	the,arteries
R1478	T5032	T5030	nsubj	arteries,budded
R1479	T5033	T5032	amod	left,arteries
R148	T663	T661	cc	and,migration
R1480	T5034	T5032	compound	carotid,arteries
R1481	T5035	T5030	prep	from,budded
R1482	T5036	T5037	det	the,aspects
R1483	T5037	T5035	pobj	aspects,from
R1484	T5038	T5037	amod	ventral,aspects
R1485	T5039	T5038	cc	or,ventral
R1486	T5040	T5041	amod	right,ventral
R1487	T5041	T5038	conj	ventral,ventral
R1488	T5042	T5037	prep	of,aspects
R1489	T5043	T5044	det	the,trunk
R149	T664	T665	amod	persistent,arteriosus
R1490	T5044	T5042	pobj	trunk,of
R1491	T5045	T5046	punct	(,1I
R1492	T5046	T5005	parataxis	1I,displayed
R1493	T5047	T5046	compound	Fig.,1I
R1494	T5048	T5049	punct	–,L
R1495	T5049	T5046	prep	L,1I
R1496	T5050	T5046	punct	),1I
R1497	T5051	T5005	punct	.,displayed
R1498	T5053	T5054	preconj	Both,arteries
R1499	T5054	T5059	nsubj	arteries,originated
R150	T665	T661	conj	arteriosus,migration
R1500	T5055	T5054	amod	right,arteries
R1501	T5056	T5055	cc	and,right
R1502	T5057	T5055	conj	left,right
R1503	T5058	T5054	amod	subclavian,arteries
R1504	T5060	T5059	advmod	consistently,originated
R1505	T5061	T5059	prep	from,originated
R1506	T5062	T5063	det	the,part
R1507	T5063	T5061	pobj	part,from
R1508	T5064	T5063	amod	descending,part
R1509	T5065	T5063	prep	of,part
R151	T666	T665	compound	truncus,arteriosus
R1510	T5066	T5067	det	the,arch
R1511	T5067	T5065	pobj	arch,of
R1512	T5068	T5067	amod	aortic,arch
R1513	T5069	T5059	punct	.,originated
R1514	T5071	T5072	advmod	Similarly,branched
R1515	T5073	T5072	punct	", ",branched
R1516	T5074	T5072	prep	in,branched
R1517	T5075	T5076	det	all,mutants
R1518	T5076	T5074	pobj	mutants,in
R1519	T5077	T5078	preconj	both,left
R152	T667	T633	punct	.,leads
R1520	T5078	T5079	amod	left,arteries
R1521	T5079	T5072	nsubj	arteries,branched
R1522	T5080	T5078	cc	and,left
R1523	T5081	T5078	conj	right,left
R1524	T5082	T5079	amod	pulmonary,arteries
R1525	T5083	T5072	advmod	always,branched
R1526	T5084	T5072	prt	out,branched
R1527	T5085	T5072	prep	from,branched
R1528	T5086	T5087	det	the,trunk
R1529	T5087	T5085	pobj	trunk,from
R153	T669	T670	mark	While,required
R1530	T5088	T5087	amod	common,trunk
R1531	T5089	T5087	amod	arterial,trunk
R1532	T5090	T5072	punct	.,branched
R1533	T5092	T5093	aux	To,conclude
R1534	T5093	T5094	advcl	conclude,displayed
R1535	T5095	T5094	punct	", ",displayed
R1536	T5096	T5097	compound	Alk5,Cre
R1537	T5097	T5101	compound	Cre,mutants
R1538	T5098	T5097	punct	/,Cre
R1539	T5099	T5097	compound	Wnt1,Cre
R154	T670	T674	advcl	required,demonstrate
R1540	T5100	T5097	punct	-,Cre
R1541	T5101	T5094	nsubj	mutants,displayed
R1542	T5102	T5094	advmod	consistently,displayed
R1543	T5103	T5094	dobj	PTA,displayed
R1544	T5104	T5103	punct	", ",PTA
R1545	T5105	T5106	dep	which,differed
R1546	T5106	T5103	relcl	differed,PTA
R1547	T5107	T5106	advmod	significantly,differed
R1548	T5108	T5106	prep	from,differed
R1549	T5109	T5110	det	the,phenotype
R155	T671	T670	nsubjpass	ALK5,required
R1550	T5110	T5108	pobj	phenotype,from
R1551	T5111	T5110	amod	characteristic,phenotype
R1552	T5112	T5113	amod	interrupted,arch
R1553	T5113	T5110	compound	arch,phenotype
R1554	T5114	T5113	amod	aortic,arch
R1555	T5115	T5110	acl	seen,phenotype
R1556	T5116	T5115	prep	in,seen
R1557	T5117	T5118	compound	Tgfbr2,Cre
R1558	T5118	T5122	compound	Cre,mutants
R1559	T5119	T5118	punct	/,Cre
R156	T672	T670	auxpass	is,required
R1560	T5120	T5118	compound	Wnt1,Cre
R1561	T5121	T5118	punct	-,Cre
R1562	T5122	T5116	pobj	mutants,in
R1563	T5123	T5124	punct	[,9
R1564	T5124	T5094	parataxis	9,displayed
R1565	T5125	T5124	nummod	8,9
R1566	T5126	T5124	punct	",",9
R1567	T5127	T5124	punct	],9
R1568	T5128	T5094	punct	.,displayed
R157	T673	T670	neg	not,required
R1575	T5938	T5939	amod	Abnormal,patterning
R1576	T5940	T5939	prep	of,patterning
R1577	T5941	T5942	det	the,arteries
R1578	T5942	T5940	pobj	arteries,of
R1579	T5943	T5942	amod	pharyngeal,arteries
R158	T675	T670	prep	for,required
R1580	T5944	T5942	compound	arch,arteries
R1581	T5945	T5942	cc	and,arteries
R1582	T5946	T5947	amod	aortic,sac
R1583	T5947	T5942	conj	sac,arteries
R1584	T5948	T5939	prep	in,patterning
R1585	T5949	T5950	compound	Alk5,Wnt1Cre
R1586	T5950	T5952	compound	Wnt1Cre,mutants
R1587	T5951	T5950	punct	/,Wnt1Cre
R1588	T5952	T5948	pobj	mutants,in
R1589	T5954	T5955	prep	During,undergo
R159	T676	T677	amod	neural,cell
R1590	T5956	T5957	amod	cardiovascular,development
R1591	T5957	T5954	pobj	development,During
R1592	T5958	T5955	punct	", ",undergo
R1593	T5959	T5960	det	the,PAAs
R1594	T5960	T5955	nsubj	PAAs,undergo
R1595	T5961	T5962	det	a,set
R1596	T5962	T5955	dobj	set,undergo
R1597	T5963	T5962	amod	complex,set
R1598	T5964	T5962	prep	of,set
R1599	T5965	T5966	amod	sequential,steps
R16	T514	T517	nsubj	signaling,leads
R160	T677	T679	compound	cell,migration
R1600	T5966	T5964	pobj	steps,of
R1601	T5967	T5966	amod	asymmetric,steps
R1602	T5968	T5966	compound	remodeling,steps
R1603	T5969	T5962	acl	resulting,set
R1604	T5970	T5969	prep	in,resulting
R1605	T5971	T5972	det	the,arch
R1606	T5972	T5970	pobj	arch,in
R1607	T5973	T5974	amod	left,sided
R1608	T5974	T5972	amod	sided,arch
R1609	T5975	T5974	punct	-,sided
R161	T678	T677	compound	crest,cell
R1610	T5976	T5972	amod	aortic,arch
R1611	T5977	T5955	punct	.,undergo
R1612	T5979	T5980	aux	To,determine
R1613	T5980	T5981	advcl	determine,performed
R1614	T5982	T5980	punct	", ",determine
R1615	T5983	T5984	mark	whether,involved
R1616	T5984	T5980	ccomp	involved,determine
R1617	T5985	T5986	npadvmod	ALK5,mediated
R1618	T5986	T5988	amod	mediated,signaling
R1619	T5987	T5986	punct	-,mediated
R162	T679	T675	pobj	migration,for
R1620	T5988	T5984	nsubjpass	signaling,involved
R1621	T5989	T5984	auxpass	was,involved
R1622	T5990	T5984	prep	in,involved
R1623	T5991	T5990	pobj	remodeling,in
R1624	T5992	T5991	prep	of,remodeling
R1625	T5993	T5992	pobj	PAAs,of
R1626	T5994	T5981	punct	", ",performed
R1627	T5995	T5981	nsubj	we,performed
R1628	T5996	T5997	amod	intracardiac,injections
R1629	T5997	T5981	dobj	injections,performed
R163	T680	T674	punct	", ",demonstrate
R1630	T5998	T5999	compound	India,ink
R1631	T5999	T5997	compound	ink,injections
R1632	T6000	T5981	prep	at,performed
R1633	T6001	T6002	amod	different,stages
R1634	T6002	T6000	pobj	stages,at
R1635	T6003	T6002	amod	developmental,stages
R1636	T6004	T5981	punct	.,performed
R1637	T6006	T6007	mark	While,show
R1638	T6007	T6019	advcl	show,became
R1639	T6008	T6007	prep	at,show
R164	T681	T682	poss	our,results
R1640	T6009	T6008	pobj	E10,at
R1641	T6010	T6007	punct	", ",show
R1642	T6011	T6012	compound	Alk5,Cre
R1643	T6012	T6016	compound	Cre,mutants
R1644	T6013	T6012	punct	/,Cre
R1645	T6014	T6012	compound	Wnt1,Cre
R1646	T6015	T6012	punct	-,Cre
R1647	T6016	T6007	nsubj	mutants,show
R1648	T6017	T6007	aux	did,show
R1649	T6018	T6007	neg	not,show
R165	T682	T674	nsubj	results,demonstrate
R1650	T6020	T6021	amod	obvious,differences
R1651	T6021	T6007	dobj	differences,show
R1652	T6022	T6021	prep	in,differences
R1653	T6023	T6024	det	the,PAAs
R1654	T6024	T6022	pobj	PAAs,in
R1655	T6025	T6019	punct	", ",became
R1656	T6026	T6027	amod	abnormal,remodeling
R1657	T6027	T6019	nsubj	remodeling,became
R1658	T6028	T6019	acomp	obvious,became
R1659	T6029	T6019	prep	in,became
R166	T683	T684	mark	that,plays
R1660	T6030	T6029	pobj	mutants,in
R1661	T6031	T6032	det	a,day
R1662	T6032	T6033	npadvmod	day,later
R1663	T6033	T6019	advmod	later,became
R1664	T6034	T6019	prep	at,became
R1665	T6035	T6034	pobj	E11,at
R1666	T6036	T6037	punct	(,Fig.
R1667	T6037	T6019	parataxis	Fig.,became
R1668	T6038	T6037	nummod	2,Fig.
R1669	T6039	T6037	punct	),Fig.
R167	T684	T674	ccomp	plays,demonstrate
R1670	T6040	T6019	punct	.,became
R1671	T6042	T6043	det	The,controls
R1672	T6043	T6044	nsubj	controls,displayed
R1673	T6045	T6046	det	the,PAAs
R1674	T6046	T6044	dobj	PAAs,displayed
R1675	T6047	T6048	advmod	well,formed
R1676	T6048	T6046	amod	formed,PAAs
R1677	T6049	T6048	punct	-,formed
R1678	T6050	T6046	nmod	3rd,PAAs
R1679	T6051	T6050	punct	", ",3rd
R168	T685	T684	nsubj	it,plays
R1680	T6052	T6050	conj	4th,3rd
R1681	T6053	T6052	cc	and,4th
R1682	T6054	T6052	conj	6th,4th
R1683	T6055	T6044	punct	.,displayed
R1684	T6057	T6058	advmod	Moreover,regressing
R1685	T6059	T6058	punct	", ",regressing
R1686	T6060	T6061	det	the,duct
R1687	T6061	T6058	nsubj	duct,regressing
R1688	T6062	T6061	amod	carotid,duct
R1689	T6063	T6061	punct	(,duct
R169	T686	T687	det	an,role
R1690	T6064	T6065	det	the,aorta
R1691	T6065	T6061	appos	aorta,duct
R1692	T6066	T6065	amod	dorsal,aorta
R1693	T6067	T6065	prep	between,aorta
R1694	T6068	T6069	det	the,PAAs
R1695	T6069	T6067	pobj	PAAs,between
R1696	T6070	T6069	amod	3rd,PAAs
R1697	T6071	T6070	cc	and,3rd
R1698	T6072	T6070	conj	4th,3rd
R1699	T6073	T6058	punct	),regressing
R17	T516	T514	compound	ALK5,signaling
R170	T687	T684	dobj	role,plays
R1700	T6074	T6058	aux	was,regressing
R1701	T6075	T6058	advmod	already,regressing
R1702	T6076	T6077	mark	as,demonstrated
R1703	T6077	T6058	advcl	demonstrated,regressing
R1704	T6078	T6077	agent	by,demonstrated
R1705	T6079	T6080	det	the,size
R1706	T6080	T6078	pobj	size,by
R1707	T6081	T6080	amod	reduced,size
R1708	T6082	T6083	punct	(,2A
R1709	T6083	T6077	parataxis	2A,demonstrated
R171	T688	T687	amod	important,role
R1710	T6084	T6083	compound	Fig.,2A
R1711	T6085	T6083	punct	),2A
R1712	T6086	T6058	punct	.,regressing
R1713	T6088	T6089	prep	In,were
R1714	T6090	T6091	compound	Alk5,Cre
R1715	T6091	T6095	compound	Cre,mutants
R1716	T6092	T6091	punct	/,Cre
R1717	T6093	T6091	compound	Wnt1,Cre
R1718	T6094	T6091	punct	-,Cre
R1719	T6095	T6088	pobj	mutants,In
R172	T689	T684	prep	in,plays
R1720	T6096	T6089	punct	", ",were
R1721	T6097	T6098	det	the,pairs
R1722	T6098	T6089	nsubj	pairs,were
R1723	T6099	T6098	amod	3rd,pairs
R1724	T6100	T6099	cc	and,3rd
R1725	T6101	T6099	conj	4th,3rd
R1726	T6102	T6098	prep	of,pairs
R1727	T6103	T6102	pobj	PAAs,of
R1728	T6104	T6105	advmod	bilaterally,hypoplastic
R1729	T6105	T6089	acomp	hypoplastic,were
R173	T690	T691	det	the,survival
R1730	T6106	T6089	punct	", ",were
R1731	T6107	T6108	mark	whereas,was
R1732	T6108	T6089	advcl	was,were
R1733	T6109	T6110	det	the,pair
R1734	T6110	T6108	nsubj	pair,was
R1735	T6111	T6110	amod	6th,pair
R1736	T6112	T6110	prep	of,pair
R1737	T6113	T6112	pobj	PAAs,of
R1738	T6114	T6115	advmod	notably,hyperplastic
R1739	T6115	T6108	acomp	hyperplastic,was
R174	T691	T689	pobj	survival,in
R1740	T6116	T6117	punct	(,2B
R1741	T6117	T6108	parataxis	2B,was
R1742	T6118	T6117	compound	Fig.,2B
R1743	T6119	T6117	punct	),2B
R1744	T6120	T6089	punct	.,were
R1745	T6122	T6123	advmod	Furthermore,was
R1746	T6124	T6123	punct	", ",was
R1747	T6125	T6126	det	the,duct
R1748	T6126	T6123	nsubj	duct,was
R1749	T6127	T6126	amod	carotid,duct
R175	T692	T691	prep	of,survival
R1750	T6128	T6129	advmod	remarkably,large
R1751	T6129	T6123	acomp	large,was
R1752	T6130	T6123	punct	", ",was
R1753	T6131	T6132	advmod	when,compared
R1754	T6132	T6123	advcl	compared,was
R1755	T6133	T6132	prep	to,compared
R1756	T6134	T6133	pobj	controls,to
R1757	T6135	T6123	punct	.,was
R1758	T6137	T6138	mark	While,displayed
R1759	T6138	T6141	advcl	displayed,demonstrated
R176	T693	T694	amod	post-migratory,cells
R1760	T6139	T6140	det	the,controls
R1761	T6140	T6138	nsubj	controls,displayed
R1762	T6142	T6143	det	an,interruption
R1763	T6143	T6138	dobj	interruption,displayed
R1764	T6144	T6143	prep	of,interruption
R1765	T6145	T6146	det	the,duct
R1766	T6146	T6144	pobj	duct,of
R1767	T6147	T6146	amod	carotid,duct
R1768	T6148	T6138	prep	at,displayed
R1769	T6149	T6148	pobj	E12,at
R177	T694	T692	pobj	cells,of
R1770	T6150	T6149	cc	and,E12
R1771	T6151	T6149	conj	E13,E12
R1772	T6152	T6153	mark	as,expected
R1773	T6153	T6138	advcl	expected,displayed
R1774	T6154	T6155	punct	(,2C
R1775	T6155	T6138	parataxis	2C,displayed
R1776	T6156	T6155	compound	Fig.,2C
R1777	T6157	T6155	punct	),2C
R1778	T6158	T6141	punct	", ",demonstrated
R1779	T6159	T6160	det	the,mutants
R178	T695	T696	amod	cardiac,crest
R1780	T6160	T6141	nsubj	mutants,demonstrated
R1781	T6161	T6162	det	an,break
R1782	T6162	T6141	dobj	break,demonstrated
R1783	T6163	T6162	amod	uncharacteristic,break
R1784	T6164	T6162	prep	of,break
R1785	T6165	T6166	det	the,aorta
R1786	T6166	T6164	pobj	aorta,of
R1787	T6167	T6166	amod	dorsal,aorta
R1788	T6168	T6162	prep	between,break
R1789	T6169	T6170	det	the,pairs
R179	T696	T694	compound	crest,cells
R1790	T6170	T6168	pobj	pairs,between
R1791	T6171	T6170	amod	4th,pairs
R1792	T6172	T6171	cc	and,4th
R1793	T6173	T6171	conj	6th,4th
R1794	T6174	T6170	prep	of,pairs
R1795	T6175	T6174	pobj	PAAs,of
R1796	T6176	T6177	punct	(,2D
R1797	T6177	T6141	parataxis	2D,demonstrated
R1798	T6178	T6177	compound	Fig.,2D
R1799	T6179	T6177	punct	),2D
R18	T518	T514	prep	in,signaling
R180	T697	T696	amod	neural,crest
R1800	T6180	T6141	punct	.,demonstrated
R1801	T6182	T6183	prep	Around,forms
R1802	T6184	T6182	pobj	E11.5,Around
R1803	T6185	T6183	punct	", ",forms
R1804	T6186	T6187	det	the,sac
R1805	T6187	T6183	nsubj	sac,forms
R1806	T6188	T6187	amod	aortic,sac
R1807	T6189	T6183	advmod	normally,forms
R1808	T6190	T6191	det	a,structure
R1809	T6191	T6183	dobj	structure,forms
R181	T698	T674	punct	.,demonstrate
R1810	T6192	T6191	amod	distinctive,structure
R1811	T6193	T6194	npadvmod	T,shaped
R1812	T6194	T6191	amod	shaped,structure
R1813	T6195	T6194	punct	-,shaped
R1814	T6196	T6183	punct	", ",forms
R1815	T6197	T6198	mark	as,seen
R1816	T6198	T6183	advcl	seen,forms
R1817	T6199	T6198	prep	in,seen
R1818	T6200	T6201	amod	frontal,sections
R1819	T6201	T6199	pobj	sections,in
R182	T702	T703	poss	Our,results
R1820	T6202	T6201	prep	of,sections
R1821	T6203	T6204	det	the,sample
R1822	T6204	T6202	pobj	sample,of
R1823	T6205	T6204	compound	control,sample
R1824	T6206	T6198	prep	in,seen
R1825	T6207	T6206	pobj	Fig.,in
R1826	T6208	T6207	nummod	3,Fig.
R1827	T6209	T6210	punct	(,C
R1828	T6210	T6183	parataxis	C,forms
R1829	T6211	T6210	nmod	A,C
R183	T703	T704	nsubj	results,demonstrate
R1830	T6212	T6210	punct	",",C
R1831	T6213	T6210	punct	),C
R1832	T6214	T6183	punct	.,forms
R1833	T6216	T6217	advmod	Subsequently,transforms
R1834	T6218	T6217	punct	", ",transforms
R1835	T6219	T6220	det	the,horn
R1836	T6220	T6217	nsubj	horn,transforms
R1837	T6221	T6220	amod	right,horn
R1838	T6222	T6220	prep	of,horn
R1839	T6223	T6224	det	this,structure
R184	T705	T706	mark	that,plays
R1840	T6224	T6222	pobj	structure,of
R1841	T6225	T6217	prep	into,transforms
R1842	T6226	T6227	det	the,artery
R1843	T6227	T6225	pobj	artery,into
R1844	T6228	T6227	amod	prospective,artery
R1845	T6229	T6227	amod	brachiocephalic,artery
R1846	T6230	T6217	punct	", ",transforms
R1847	T6231	T6232	mark	while,gives
R1848	T6232	T6217	advcl	gives,transforms
R1849	T6233	T6234	det	the,horn
R185	T706	T704	ccomp	plays,demonstrate
R1850	T6234	T6232	nsubj	horn,gives
R1851	T6235	T6234	amod	left,horn
R1852	T6236	T6237	advmod	together,with
R1853	T6237	T6234	prep	with,horn
R1854	T6238	T6239	det	the,PAA
R1855	T6239	T6237	pobj	PAA,with
R1856	T6240	T6239	amod	left,PAA
R1857	T6241	T6239	amod	4th,PAA
R1858	T6242	T6232	dobj	rise,gives
R1859	T6243	T6232	prep	to,gives
R186	T707	T708	npadvmod	ALK5,mediated
R1860	T6244	T6245	det	the,arch
R1861	T6245	T6243	pobj	arch,to
R1862	T6246	T6245	amod	definitive,arch
R1863	T6247	T6245	amod	aortic,arch
R1864	T6248	T6249	punct	[,23
R1865	T6249	T6232	parataxis	23,gives
R1866	T6250	T6249	punct	],23
R1867	T6251	T6217	punct	.,transforms
R1868	T6253	T6254	prep	In,failed
R1869	T6255	T6256	compound	Alk5,Cre
R187	T708	T710	amod	mediated,signaling
R1870	T6256	T6260	compound	Cre,mutants
R1871	T6257	T6256	punct	/,Cre
R1872	T6258	T6256	compound	Wnt1,Cre
R1873	T6259	T6256	punct	-,Cre
R1874	T6260	T6253	pobj	mutants,In
R1875	T6261	T6254	punct	", ",failed
R1876	T6262	T6263	det	the,sac
R1877	T6263	T6254	nsubj	sac,failed
R1878	T6264	T6265	npadvmod	T,shaped
R1879	T6265	T6263	amod	shaped,sac
R188	T709	T708	punct	-,mediated
R1880	T6266	T6265	punct	-,shaped
R1881	T6267	T6263	amod	aortic,sac
R1882	T6268	T6269	aux	to,form
R1883	T6269	T6254	xcomp	form,failed
R1884	T6270	T6271	punct	(,D
R1885	T6271	T6269	parataxis	D,form
R1886	T6272	T6271	nmod	Fig.,D
R1887	T6273	T6271	nmod	3B,D
R1888	T6274	T6271	punct	",",D
R1889	T6275	T6271	punct	),D
R189	T710	T706	nsubj	signaling,plays
R1890	T6276	T6254	punct	.,failed
R1891	T6278	T6279	advmod	Instead,bifurcated
R1892	T6280	T6279	punct	", ",bifurcated
R1893	T6281	T6282	det	the,truncus
R1894	T6282	T6279	nsubj	truncus,bifurcated
R1895	T6283	T6279	prep	to,bifurcated
R1896	T6284	T6285	det	a,arm
R1897	T6285	T6283	pobj	arm,to
R1898	T6286	T6285	amod	left,arm
R1899	T6287	T6286	cc	and,left
R19	T519	T520	det	the,crest
R190	T711	T710	prep	in,signaling
R1900	T6288	T6286	conj	right,left
R1901	T6289	T6285	punct	", ",arm
R1902	T6290	T6291	dep	which,branched
R1903	T6291	T6285	relcl	branched,arm
R1904	T6292	T6291	advmod	further,branched
R1905	T6293	T6291	prep	to,branched
R1906	T6294	T6295	det	the,PAAs
R1907	T6295	T6293	pobj	PAAs,to
R1908	T6296	T6293	punct	", ",to
R1909	T6297	T6298	advmod	particularly,to
R191	T712	T713	amod	neural,cells
R1910	T6298	T6293	prep	to,to
R1911	T6299	T6300	det	the,pair
R1912	T6300	T6298	pobj	pair,to
R1913	T6301	T6300	amod	predominant,pair
R1914	T6302	T6300	prep	of,pair
R1915	T6303	T6304	amod	6th,PAAs
R1916	T6304	T6302	pobj	PAAs,of
R1917	T6305	T6306	punct	(,D
R1918	T6306	T6279	parataxis	D,bifurcated
R1919	T6307	T6306	nmod	Fig.,D
R192	T713	T711	pobj	cells,in
R1920	T6308	T6306	nmod	3B,D
R1921	T6309	T6306	punct	",",D
R1922	T6310	T6306	punct	),D
R1923	T6311	T6279	punct	.,bifurcated
R1924	T6313	T6314	det	The,phenotype
R1925	T6314	T6316	nsubj	phenotype,is
R1926	T6315	T6314	amod	observed,phenotype
R1927	T6317	T6316	acomp	consistent,is
R1928	T6318	T6317	prep	with,consistent
R1929	T6319	T6320	det	the,absence
R193	T714	T713	compound	crest,cells
R1930	T6320	T6318	pobj	absence,with
R1931	T6321	T6320	cc	or,absence
R1932	T6322	T6323	amod	severe,hypoplasia
R1933	T6323	T6320	conj	hypoplasia,absence
R1934	T6324	T6320	prep	of,absence
R1935	T6325	T6324	pobj	structures,of
R1936	T6326	T6325	acl	derived,structures
R1937	T6327	T6326	prep	from,derived
R1938	T6328	T6329	det	the,sac
R1939	T6329	T6327	pobj	sac,from
R194	T715	T716	det	an,role
R1940	T6330	T6329	amod	aortic,sac
R1941	T6331	T6326	prep	in,derived
R1942	T6332	T6333	amod	late,embryos
R1943	T6333	T6331	pobj	embryos,in
R1944	T6334	T6333	compound	stage,embryos
R1945	T6335	T6336	punct	(,E17
R1946	T6336	T6333	parataxis	E17,embryos
R1947	T6337	T6336	punct	),E17
R1948	T6338	T6325	punct	", ",structures
R1949	T6339	T6340	advmod	e.g.,artery
R195	T716	T706	dobj	role,plays
R1950	T6340	T6325	appos	artery,structures
R1951	T6341	T6340	punct	", ",artery
R1952	T6342	T6340	det	the,artery
R1953	T6343	T6340	amod	missing,artery
R1954	T6344	T6340	amod	brachiocephalic,artery
R1955	T6345	T6340	cc	and,artery
R1956	T6346	T6347	amod	severe,shortening
R1957	T6347	T6340	conj	shortening,artery
R1958	T6348	T6347	prep	of,shortening
R1959	T6349	T6350	det	the,truncus
R196	T717	T716	amod	essential,role
R1960	T6350	T6348	pobj	truncus,of
R1961	T6351	T6350	amod	ascending,truncus
R1962	T6352	T6353	mark	as,shown
R1963	T6353	T6316	advcl	shown,is
R1964	T6354	T6353	prep	in,shown
R1965	T6355	T6356	det	the,Figure
R1966	T6356	T6354	pobj	Figure,in
R1967	T6357	T6356	nummod	1,Figure
R1968	T6358	T6316	punct	.,is
R1969	T6831	T6832	amod	Cardiac,NCCs
R197	T718	T719	npadvmod	cell,autonomous
R1970	T6832	T6833	nsubj	NCCs,populate
R1971	T6834	T6832	amod	deficient,NCCs
R1972	T6835	T6834	prep	in,deficient
R1973	T6836	T6835	pobj	Alk5,in
R1974	T6837	T6833	aux	can,populate
R1975	T6838	T6839	det	the,tract
R1976	T6839	T6833	dobj	tract,populate
R1977	T6840	T6839	compound	outflow,tract
R1978	T6842	T6843	advmod	Next,used
R1979	T6844	T6843	nsubj	we,used
R198	T719	T716	amod	autonomous,role
R1980	T6845	T6846	det	the,assay
R1981	T6846	T6843	dobj	assay,used
R1982	T6847	T6846	nmod	R26R,assay
R1983	T6848	T6846	nmod	lineage,assay
R1984	T6849	T6848	punct	-,lineage
R1985	T6850	T6848	amod	tracing,lineage
R1986	T6851	T6852	aux	to,determine
R1987	T6852	T6843	advcl	determine,used
R1988	T6853	T6854	mark	whether,populate
R1989	T6854	T6852	ccomp	populate,determine
R199	T720	T719	punct	-,autonomous
R1990	T6855	T6854	nsubj	CNCCs,populate
R1991	T6856	T6854	aux	could,populate
R1992	T6857	T6854	advmod	appropriately,populate
R1993	T6858	T6859	det	the,region
R1994	T6859	T6854	dobj	region,populate
R1995	T6860	T6861	compound	outflow,tract
R1996	T6861	T6859	compound	tract,region
R1997	T6862	T6843	punct	.,used
R1998	T6864	T6865	advmod	Briefly,crossed
R1999	T6866	T6865	punct	", ",crossed
R20	T520	T518	pobj	crest,in
R200	T721	T716	prep	in,role
R2000	T6867	T6868	compound	Alk5Flox,Flox
R2001	T6868	T6870	compound	Flox,mice
R2002	T6869	T6868	punct	/,Flox
R2003	T6870	T6865	nsubjpass	mice,crossed
R2004	T6871	T6865	auxpass	were,crossed
R2005	T6872	T6865	prep	with,crossed
R2006	T6873	T6874	det	the,mice
R2007	T6874	T6872	pobj	mice,with
R2008	T6875	T6876	compound	ROSA26,reporter
R2009	T6876	T6874	compound	reporter,mice
R201	T722	T723	det	the,development
R2010	T6877	T6876	compound	Cre,reporter
R2011	T6878	T6865	punct	", ",crossed
R2012	T6879	T6865	cc	and,crossed
R2013	T6880	T6881	advmod	subsequently,crossed
R2014	T6881	T6865	conj	crossed,crossed
R2015	T6882	T6883	nmod	Alk5Flox,Flox
R2016	T6883	T6885	nmod	Flox,+
R2017	T6884	T6883	punct	/,Flox
R2018	T6885	T6891	punct	+,females
R2019	T6886	T6885	punct	;,+
R202	T723	T721	pobj	development,in
R2020	T6887	T6885	nmod	R26R,+
R2021	T6888	T6885	punct	(,+
R2022	T6889	T6885	punct	+,+
R2023	T6890	T6885	punct	/,+
R2024	T6891	T6881	nsubjpass	females,crossed
R2025	T6892	T6885	punct	),+
R2026	T6893	T6881	auxpass	were,crossed
R2027	T6894	T6881	prep	with,crossed
R2028	T6895	T6896	nmod	Alk5KO,Cre
R2029	T6896	T6902	compound	Cre,males
R203	T724	T725	amod	pharyngeal,tract
R2030	T6897	T6896	punct	/,Cre
R2031	T6898	T6896	nmod	WT,Cre
R2032	T6899	T6896	punct	;,Cre
R2033	T6900	T6896	compound	Wnt1,Cre
R2034	T6901	T6896	punct	-,Cre
R2035	T6902	T6894	pobj	males,with
R2036	T6903	T6881	punct	.,crossed
R2037	T6905	T6906	det	The,embryos
R2038	T6906	T6908	nsubj	embryos,had
R2039	T6907	T6906	amod	resulting,embryos
R204	T725	T723	compound	tract,development
R2040	T6909	T6910	det	the,lineage
R2041	T6910	T6913	nsubj	lineage,labeled
R2042	T6911	T6910	compound	NC,lineage
R2043	T6912	T6910	punct	-,lineage
R2044	T6913	T6908	ccomp	labeled,had
R2045	T6914	T6913	advmod	permanently,labeled
R2046	T6915	T6913	prep	with,labeled
R2047	T6916	T6917	compound	β,expression
R2048	T6917	T6915	pobj	expression,with
R2049	T6918	T6917	punct	-,expression
R205	T726	T724	cc	and,pharyngeal
R2050	T6919	T6917	compound	galactosidase,expression
R2051	T6920	T6908	punct	", ",had
R2052	T6921	T6908	cc	and,had
R2053	T6922	T6908	conj	displayed,had
R2054	T6923	T6924	amod	identical,phenotypes
R2055	T6924	T6922	dobj	phenotypes,displayed
R2056	T6925	T6924	prep	to,phenotypes
R2057	T6926	T6925	pobj	those,to
R2058	T6927	T6926	acl	obtained,those
R2059	T6928	T6927	prep	without,obtained
R206	T727	T724	conj	cardiac,pharyngeal
R2060	T6929	T6930	det	the,reporter
R2061	T6930	T6928	pobj	reporter,without
R2062	T6931	T6930	compound	R26R,reporter
R2063	T6932	T6908	punct	.,had
R2064	T6934	T6935	nsubj	Staining,reveal
R2065	T6936	T6934	prep	of,Staining
R2066	T6937	T6936	pobj	embryos,of
R2067	T6938	T6934	prep	for,Staining
R2068	T6939	T6940	compound	β,galactosidase
R2069	T6940	T6938	pobj	galactosidase,for
R207	T728	T725	compound	outflow,tract
R2070	T6941	T6940	punct	-,galactosidase
R2071	T6942	T6934	prep	at,Staining
R2072	T6943	T6942	pobj	E8,at
R2073	T6944	T6945	punct	-,E11
R2074	T6945	T6943	prep	E11,E8
R2075	T6946	T6935	aux	did,reveal
R2076	T6947	T6935	neg	not,reveal
R2077	T6948	T6949	amod	detectable,differences
R2078	T6949	T6935	dobj	differences,reveal
R2079	T6950	T6949	prep	in,differences
R208	T729	T704	punct	.,demonstrate
R2080	T6951	T6952	compound	NCC,migration
R2081	T6952	T6950	pobj	migration,in
R2082	T6953	T6949	prep	between,differences
R2083	T6954	T6953	pobj	mutants,between
R2084	T6955	T6954	cc	and,mutants
R2085	T6956	T6954	conj	controls,mutants
R2086	T6957	T6958	punct	(,shown
R2087	T6958	T6935	parataxis	shown,reveal
R2088	T6959	T6958	nsubj	data,shown
R2089	T6960	T6958	neg	not,shown
R209	T513	T514	amod	Defective,signaling
R2090	T6961	T6958	punct	),shown
R2091	T6962	T6935	punct	.,reveal
R2092	T6964	T6965	advmod	Similarly,demonstrated
R2093	T6966	T6965	punct	", ",demonstrated
R2094	T6967	T6968	amod	serial,sectioning
R2095	T6968	T6965	nsubj	sectioning,demonstrated
R2096	T6969	T6968	amod	transverse,sectioning
R2097	T6970	T6968	prep	of,sectioning
R2098	T6971	T6972	amod	whole,mount
R2099	T6972	T6973	compound	mount,embryos
R21	T521	T520	amod	neural,crest
R2100	T6973	T6970	pobj	embryos,of
R2101	T6974	T6975	punct	(,E10
R2102	T6975	T6968	parataxis	E10,sectioning
R2103	T6976	T6977	punct	-,E12
R2104	T6977	T6975	prep	E12,E10
R2105	T6978	T6975	punct	),E10
R2106	T6979	T6968	cc	and,sectioning
R2107	T6980	T6981	amod	subsequent,analysis
R2108	T6981	T6968	conj	analysis,sectioning
R2109	T6982	T6981	prep	of,analysis
R2110	T6983	T6984	advmod	positively,stained
R2111	T6984	T6985	amod	stained,cells
R2112	T6985	T6982	pobj	cells,of
R2113	T6986	T6985	prep	in,cells
R2114	T6987	T6988	det	the,region
R2115	T6988	T6986	pobj	region,in
R2116	T6989	T6988	compound	OFT,region
R2117	T6990	T6991	mark	that,were
R2118	T6991	T6965	ccomp	were,demonstrated
R2119	T6992	T6991	nsubj	CNCCs,were
R2120	T6993	T6992	amod	deficient,CNCCs
R2121	T6994	T6993	prep	in,deficient
R2122	T6995	T6994	pobj	Alk5,in
R2123	T6996	T6991	acomp	capable,were
R2124	T6997	T6996	prep	of,capable
R2125	T6998	T6997	pcomp	populating,of
R2126	T6999	T7000	det	the,PAAs
R2127	T7000	T6998	dobj	PAAs,populating
R2128	T7001	T7000	punct	", ",PAAs
R2129	T7002	T7003	amod	aortic,sac
R2130	T7003	T7000	conj	sac,PAAs
R2131	T7004	T7003	cc	and,sac
R2132	T7005	T7006	amod	conotruncal,ridges
R2133	T7006	T7003	conj	ridges,sac
R2134	T7007	T6998	prep	at,populating
R2135	T7008	T7009	det	a,level
R2136	T7009	T7007	pobj	level,at
R2137	T7010	T7009	amod	comparable,level
R2138	T7011	T7010	prep	to,comparable
R2139	T7012	T7011	pobj	that,to
R2140	T7013	T7012	prep	of,that
R2141	T7014	T7013	pobj	controls,of
R2142	T7015	T7016	punct	(,Fig
R2143	T7016	T6998	parataxis	Fig,populating
R2144	T7017	T7016	nummod	4,Fig
R2145	T7018	T7016	punct	),Fig
R2146	T7019	T6965	punct	.,demonstrated
R2147	T7021	T7022	aux	To,conclude
R2148	T7022	T7023	advcl	conclude,were
R2149	T7024	T7023	punct	", ",were
R2150	T7025	T7026	det	the,phenotypes
R2151	T7026	T7023	nsubj	phenotypes,were
R2152	T7027	T7026	amod	observed,phenotypes
R2153	T7028	T7026	prep	in,phenotypes
R2154	T7029	T7030	compound	Alk5,Cre
R2155	T7030	T7034	compound	Cre,mutants
R2156	T7031	T7030	punct	/,Cre
R2157	T7032	T7030	compound	Wnt1,Cre
R2158	T7033	T7030	punct	-,Cre
R2159	T7034	T7028	pobj	mutants,in
R2160	T7035	T7023	advmod	certainly,were
R2161	T7036	T7023	neg	not,were
R2162	T7037	T7023	prep	due,were
R2163	T7038	T7037	pcomp	to,due
R2164	T7039	T7040	amod	defective,migration
R2165	T7040	T7037	pobj	migration,due
R2166	T7041	T7040	prep	of,migration
R2167	T7042	T7041	pobj	CNCCs,of
R2168	T7043	T7040	prep	to,migration
R2169	T7044	T7045	det	the,regions
R2170	T7045	T7043	pobj	regions,to
R2171	T7046	T7045	amod	pharyngeal,regions
R2172	T7047	T7046	cc	and,pharyngeal
R2173	T7048	T7049	compound	outflow,tract
R2174	T7049	T7046	conj	tract,pharyngeal
R2175	T7050	T7023	punct	.,were
R2182	T8381	T8382	amod	Aortic,sac
R2183	T8382	T8383	nmod	sac,defects
R2184	T8384	T8382	cc	and,sac
R2185	T8385	T8386	amod	aortico,pulmonary
R2186	T8386	T8388	amod	pulmonary,septal
R2187	T8387	T8386	punct	-,pulmonary
R2188	T8388	T8382	conj	septal,sac
R2189	T8389	T8383	prep	in,defects
R2190	T8390	T8391	compound	Alk5,Wnt1Cre
R2191	T8391	T8393	compound	Wnt1Cre,embryos
R2192	T8392	T8391	punct	/,Wnt1Cre
R2193	T8393	T8389	pobj	embryos,in
R2194	T8394	T8393	compound	mutant,embryos
R2195	T8396	T8397	nsubj	Septation,begins
R2196	T8398	T8396	prep	of,Septation
R2197	T8399	T8400	det	the,lumen
R2198	T8400	T8398	pobj	lumen,of
R2199	T8401	T8402	compound	outflow,tract
R22	T522	T517	prep	to,leads
R2200	T8402	T8400	compound	tract,lumen
R2201	T8403	T8397	prep	in,begins
R2202	T8404	T8405	det	a,direction
R2203	T8405	T8403	pobj	direction,in
R2204	T8406	T8405	amod	cranial,direction
R2205	T8407	T8406	punct	-,cranial
R2206	T8408	T8406	prep	to,cranial
R2207	T8409	T8408	punct	-,to
R2208	T8410	T8408	amod	caudal,to
R2209	T8411	T8397	punct	", ",begins
R2210	T8412	T8397	advcl	starting,begins
R2211	T8413	T8412	advmod	distally,starting
R2212	T8414	T8412	prep	in,starting
R2213	T8415	T8416	det	the,sac
R2214	T8416	T8414	pobj	sac,in
R2215	T8417	T8416	amod	aortic,sac
R2216	T8418	T8412	cc	and,starting
R2217	T8419	T8412	conj	proceeding,starting
R2218	T8420	T8419	prep	toward,proceeding
R2219	T8421	T8422	det	the,heart
R2220	T8422	T8420	pobj	heart,toward
R2221	T8423	T8424	punct	[,24
R2222	T8424	T8397	parataxis	24,begins
R2223	T8425	T8424	punct	],24
R2224	T8426	T8397	punct	.,begins
R2225	T8428	T8429	advmod	Initially,forms
R2226	T8430	T8429	punct	", ",forms
R2227	T8431	T8432	det	the,mesenchyme
R2228	T8432	T8429	nsubj	mesenchyme,forms
R2229	T8433	T8432	amod	condensed,mesenchyme
R2230	T8434	T8432	acl	derived,mesenchyme
R2231	T8435	T8434	prep	from,derived
R2232	T8436	T8437	det	the,NC
R2233	T8437	T8435	pobj	NC,from
R2234	T8438	T8429	prep	in,forms
R2235	T8439	T8440	det	the,base
R2236	T8440	T8438	pobj	base,in
R2237	T8441	T8440	prep	of,base
R2238	T8442	T8443	det	the,sac
R2239	T8443	T8441	pobj	sac,of
R2240	T8444	T8443	amod	aortic,sac
R2241	T8445	T8429	prep	between,forms
R2242	T8446	T8447	det	the,origins
R2243	T8447	T8445	pobj	origins,between
R2244	T8448	T8447	prep	of,origins
R2245	T8449	T8450	amod	4th,PAAs
R2246	T8450	T8448	pobj	PAAs,of
R2247	T8451	T8449	cc	and,4th
R2248	T8452	T8449	conj	6th,4th
R2249	T8453	T8429	punct	.,forms
R2250	T8455	T8456	advmod	Subsequently,extend
R2251	T8457	T8456	punct	", ",extend
R2252	T8458	T8459	nummod	two,prongs
R2253	T8459	T8456	nsubj	prongs,extend
R2254	T8460	T8459	prep	of,prongs
R2255	T8461	T8462	det	the,septum
R2256	T8462	T8460	pobj	septum,of
R2257	T8463	T8462	amod	developing,septum
R2258	T8464	T8465	amod	aortico,pulmonary
R2259	T8465	T8462	amod	pulmonary,septum
R2260	T8466	T8465	punct	-,pulmonary
R2261	T8467	T8468	punct	(,AP
R2262	T8468	T8465	parataxis	AP,pulmonary
R2263	T8469	T8468	punct	),AP
R2264	T8470	T8456	prep	into,extend
R2265	T8471	T8472	det	the,cushions
R2266	T8472	T8470	pobj	cushions,into
R2267	T8473	T8472	amod	truncal,cushions
R2268	T8474	T8456	cc	and,extend
R2269	T8475	T8476	det	the,complex
R2270	T8476	T8481	nsubj	complex,crosses
R2271	T8477	T8478	amod	aortico,pulmonary
R2272	T8478	T8476	amod	pulmonary,complex
R2273	T8479	T8478	punct	-,pulmonary
R2274	T8480	T8476	compound	septation,complex
R2275	T8481	T8456	conj	crosses,extend
R2276	T8482	T8483	det	the,sac
R2277	T8483	T8481	dobj	sac,crosses
R2278	T8484	T8483	amod	aortic,sac
R2279	T8485	T8481	advmod	cranially,crosses
R2280	T8486	T8481	punct	.,crosses
R2281	T8488	T8489	prep	In,seen
R2282	T8490	T8491	npadvmod	ink,injected
R2283	T8491	T8493	amod	injected,embryos
R2284	T8492	T8491	punct	-,injected
R2285	T8493	T8488	pobj	embryos,In
R2286	T8494	T8493	compound	control,embryos
R2287	T8495	T8489	prep	at,seen
R2288	T8496	T8495	pobj	E11.5,at
R2289	T8497	T8489	punct	", ",seen
R2290	T8498	T8499	det	a,transition
R2291	T8499	T8489	nsubjpass	transition,seen
R2292	T8500	T8499	amod	characteristic,transition
R2293	T8501	T8499	amod	conotruncal,transition
R2294	T8502	T8499	acl	separating,transition
R2295	T8503	T8504	det	the,truncus
R2296	T8504	T8502	dobj	truncus,separating
R2297	T8505	T8504	cc	and,truncus
R2298	T8506	T8504	conj	conus,truncus
R2299	T8507	T8489	aux	could,seen
R23	T523	T524	amod	increased,apoptosis
R2300	T8508	T8489	auxpass	be,seen
R2301	T8509	T8489	prep	as,seen
R2302	T8510	T8511	det	a,configuration
R2303	T8511	T8509	pobj	configuration,as
R2304	T8512	T8511	amod	twisted,configuration
R2305	T8513	T8511	punct	", ",configuration
R2306	T8514	T8511	acl	resulting,configuration
R2307	T8515	T8514	prep	from,resulting
R2308	T8516	T8517	det	a,change
R2309	T8517	T8515	pobj	change,from
R2310	T8518	T8517	prep	in,change
R2311	T8519	T8518	pobj	orientation,in
R2312	T8520	T8519	prep	of,orientation
R2313	T8521	T8522	det	the,cushions
R2314	T8522	T8520	pobj	cushions,of
R2315	T8523	T8522	amod	truncal,cushions
R2316	T8524	T8523	cc	and,truncal
R2317	T8525	T8523	conj	conal,truncal
R2318	T8526	T8527	punct	(,Fig.
R2319	T8527	T8514	parataxis	Fig.,resulting
R2320	T8528	T8527	nummod	5,Fig.
R2321	T8529	T8527	punct	),Fig.
R2322	T8530	T8489	punct	.,seen
R2323	T8532	T8533	prep	In,appeared
R2324	T8534	T8532	pobj	contrast,In
R2325	T8535	T8533	punct	", ",appeared
R2326	T8536	T8533	prep	in,appeared
R2327	T8537	T8538	compound	Alk5,Cre
R2328	T8538	T8542	compound	Cre,mutants
R2329	T8539	T8538	punct	/,Cre
R2330	T8540	T8538	compound	Wnt1,Cre
R2331	T8541	T8538	punct	-,Cre
R2332	T8542	T8536	pobj	mutants,in
R2333	T8543	T8544	det	the,tract
R2334	T8544	T8533	nsubj	tract,appeared
R2335	T8545	T8544	compound	outflow,tract
R2336	T8546	T8547	advmod	unusually,straight
R2337	T8547	T8533	oprd	straight,appeared
R2338	T8548	T8533	punct	", ",appeared
R2339	T8549	T8533	advcl	failing,appeared
R2340	T8550	T8551	aux	to,demonstrate
R2341	T8551	T8549	xcomp	demonstrate,failing
R2342	T8552	T8553	det	the,transition
R2343	T8553	T8551	dobj	transition,demonstrate
R2344	T8554	T8553	amod	distinct,transition
R2345	T8555	T8553	amod	conotruncal,transition
R2346	T8556	T8557	punct	(,D
R2347	T8557	T8533	parataxis	D,appeared
R2348	T8558	T8557	nmod	Fig.,D
R2349	T8559	T8557	nmod	5B,D
R2350	T8560	T8557	punct	",",D
R2351	T8561	T8557	punct	),D
R2352	T8562	T8533	punct	.,appeared
R2353	T8564	T8565	det	This,assay
R2354	T8565	T8566	nsubj	assay,showed
R2355	T8567	T8566	advmod	also,showed
R2356	T8568	T8566	advmod	clearly,showed
R2357	T8569	T8570	det	a,reduction
R2358	T8570	T8566	dobj	reduction,showed
R2359	T8571	T8570	amod	dramatic,reduction
R2360	T8572	T8570	prep	in,reduction
R2361	T8573	T8574	det	the,size
R2362	T8574	T8572	pobj	size,in
R2363	T8575	T8574	prep	of,size
R2364	T8576	T8577	det	the,sac
R2365	T8577	T8575	pobj	sac,of
R2366	T8578	T8577	amod	aortic,sac
R2367	T8579	T8566	punct	.,showed
R2368	T8581	T8582	amod	Histological,analysis
R2369	T8582	T8583	nsubj	analysis,displayed
R2370	T8584	T8582	prep	of,analysis
R2371	T8585	T8586	compound	control,samples
R2372	T8586	T8584	pobj	samples,of
R2373	T8587	T8588	det	the,rotation
R2374	T8588	T8583	dobj	rotation,displayed
R2375	T8589	T8588	amod	characteristic,rotation
R2376	T8590	T8588	prep	of,rotation
R2377	T8591	T8592	det	the,sac
R2378	T8592	T8590	pobj	sac,of
R2379	T8593	T8592	amod	aortic,sac
R2380	T8594	T8592	cc	and,sac
R2381	T8595	T8596	amod	truncal,OFT
R2382	T8596	T8592	conj	OFT,sac
R2383	T8597	T8583	prep	at,displayed
R2384	T8598	T8599	det	the,level
R2385	T8599	T8597	pobj	level,at
R2386	T8600	T8601	advmod	where,takes
R2387	T8601	T8599	relcl	takes,level
R2388	T8602	T8603	det	the,septation
R2389	T8603	T8601	nsubj	septation,takes
R2390	T8604	T8603	compound	AP,septation
R2391	T8605	T8601	dobj	place,takes
R2392	T8606	T8583	cc	and,displayed
R2393	T8607	T8583	conj	verified,displayed
R2394	T8608	T8609	det	the,presence
R2395	T8609	T8607	dobj	presence,verified
R2396	T8610	T8609	prep	of,presence
R2397	T8611	T8612	det	the,mesenchyme
R2398	T8612	T8610	pobj	mesenchyme,of
R2399	T8613	T8612	amod	distinctive,mesenchyme
R24	T524	T522	pobj	apoptosis,to
R2400	T8614	T8612	amod	condensed,mesenchyme
R2401	T8615	T8612	nmod	AP,mesenchyme
R2402	T8616	T8615	punct	-,AP
R2403	T8617	T8615	amod	septal,AP
R2404	T8618	T8612	punct	", ",mesenchyme
R2405	T8619	T8620	dep	which,divided
R2406	T8620	T8612	relcl	divided,mesenchyme
R2407	T8621	T8620	advmod	gradually,divided
R2408	T8622	T8623	det	the,OFT
R2409	T8623	T8620	dobj	OFT,divided
R2410	T8624	T8620	prep	to,divided
R2411	T8625	T8626	det	the,aorta
R2412	T8626	T8624	pobj	aorta,to
R2413	T8627	T8626	cc	and,aorta
R2414	T8628	T8629	det	the,trunk
R2415	T8629	T8626	conj	trunk,aorta
R2416	T8630	T8629	amod	pulmonary,trunk
R2417	T8631	T8632	punct	(,asterisks
R2418	T8632	T8620	parataxis	asterisks,divided
R2419	T8633	T8632	prep	in,asterisks
R2420	T8634	T8635	compound	Fig.,6A
R2421	T8635	T8633	pobj	6A,in
R2422	T8636	T8632	punct	),asterisks
R2423	T8637	T8583	punct	.,displayed
R2424	T8639	T8640	compound	R26R,tracing
R2425	T8640	T8642	nsubj	tracing,showed
R2426	T8641	T8640	compound	lineage,tracing
R2427	T8643	T8644	mark	that,derived
R2428	T8644	T8642	ccomp	derived,showed
R2429	T8645	T8646	det	this,tissue
R2430	T8646	T8644	nsubjpass	tissue,derived
R2431	T8647	T8644	auxpass	is,derived
R2432	T8648	T8644	prep	from,derived
R2433	T8649	T8650	det	the,NC
R2434	T8650	T8648	pobj	NC,from
R2435	T8651	T8644	punct	", ",derived
R2436	T8652	T8653	mark	while,showed
R2437	T8653	T8644	advcl	showed,derived
R2438	T8654	T8653	nsubj	immunostaining,showed
R2439	T8655	T8654	prep	for,immunostaining
R2440	T8656	T8657	compound	α,SMA
R2441	T8657	T8655	pobj	SMA,for
R2442	T8658	T8657	punct	-,SMA
R2443	T8659	T8653	dobj	differentiation,showed
R2444	T8660	T8659	prep	into,differentiation
R2445	T8661	T8662	amod	smooth,muscle
R2446	T8662	T8660	pobj	muscle,into
R2447	T8663	T8664	punct	(,6B
R2448	T8664	T8653	parataxis	6B,showed
R2449	T8665	T8664	compound	Fig,6B
R245	T2694	T2695	det	A,percentage
R2450	T8666	T8664	punct	),6B
R2451	T8667	T8642	punct	.,showed
R2452	T8669	T8670	prep	In,fails
R2453	T8671	T8672	compound	Alk5,Cre
R2454	T8672	T8676	compound	Cre,mutants
R2455	T8673	T8672	punct	/,Cre
R2456	T8674	T8672	compound	Wnt1,Cre
R2457	T8675	T8672	punct	-,Cre
R2458	T8676	T8669	pobj	mutants,In
R2459	T8677	T8678	det	the,rotation
R246	T2695	T2697	nsubjpass	percentage,caused
R2460	T8678	T8670	nsubj	rotation,fails
R2461	T8679	T8678	amod	characteristic,rotation
R2462	T8680	T8678	prep	of,rotation
R2463	T8681	T8682	det	the,sac
R2464	T8682	T8680	pobj	sac,of
R2465	T8683	T8682	amod	aortic,sac
R2466	T8684	T8682	cc	and,sac
R2467	T8685	T8686	amod	truncal,OFT
R2468	T8686	T8682	conj	OFT,sac
R2469	T8687	T8688	aux	to,take
R247	T2696	T2695	amod	considerable,percentage
R2470	T8688	T8670	xcomp	take,fails
R2471	T8689	T8688	dobj	place,take
R2472	T8690	T8691	punct	(,6G
R2473	T8691	T8688	parataxis	6G,take
R2474	T8692	T8691	compound	Fig.,6G
R2475	T8693	T8694	punct	–,L
R2476	T8694	T8691	prep	L,6G
R2477	T8695	T8691	punct	),6G
R2478	T8696	T8670	punct	", ",fails
R2479	T8697	T8670	cc	and,fails
R248	T2698	T2695	prep	of,percentage
R2480	T8698	T8699	det	a,septum
R2481	T8699	T8704	nsubj	septum,was
R2482	T8700	T8701	advmod	properly,formed
R2483	T8701	T8699	amod	formed,septum
R2484	T8702	T8699	compound	AP,septum
R2485	T8703	T8699	punct	-,septum
R2486	T8704	T8670	conj	was,fails
R2487	T8705	T8704	neg	not,was
R2488	T8706	T8704	acomp	detectable,was
R2489	T8707	T8708	punct	(,H
R249	T2699	T2700	amod	cardiac,defects
R2490	T8708	T8704	parataxis	H,was
R2491	T8709	T8708	nmod	Fig.,H
R2492	T8710	T8708	nmod	6G,H
R2493	T8711	T8708	punct	",",H
R2494	T8712	T8708	punct	),H
R2495	T8713	T8670	punct	.,fails
R2496	T8715	T8716	compound	R26R,tracing
R2497	T8716	T8718	nsubj	tracing,demonstrated
R2498	T8717	T8716	compound	lineage,tracing
R2499	T8719	T8720	mark	that,were
R25	T525	T524	amod	postmigratory,apoptosis
R250	T2700	T2698	pobj	defects,of
R2500	T8720	T8718	ccomp	were,demonstrated
R2501	T8721	T8722	det	the,defects
R2502	T8722	T8720	nsubj	defects,were
R2503	T8723	T8720	neg	not,were
R2504	T8724	T8720	prep	due,were
R2505	T8725	T8724	pcomp	to,due
R2506	T8726	T8724	pobj	failure,due
R2507	T8727	T8726	prep	of,failure
R2508	T8728	T8727	pobj	NCCs,of
R2509	T8729	T8730	aux	to,reach
R251	T2701	T2700	compound	birth,defects
R2510	T8730	T8726	acl	reach,failure
R2511	T8731	T8732	det	the,region
R2512	T8732	T8730	dobj	region,reach
R2513	T8733	T8732	compound	OFT,region
R2514	T8734	T8718	punct	.,demonstrated
R2515	T8736	T8737	npadvmod	NC,derived
R2516	T8737	T8739	amod	derived,cells
R2517	T8738	T8737	punct	-,derived
R2518	T8739	T8740	nsubj	cells,demonstrated
R2519	T8741	T8739	prep	around,cells
R252	T2702	T2697	auxpass	is,caused
R2520	T8742	T8743	det	the,sac
R2521	T8743	T8741	pobj	sac,around
R2522	T8744	T8745	advmod	abnomally,bifurcated
R2523	T8745	T8743	amod	bifurcated,sac
R2524	T8746	T8743	amod	aortic,sac
R2525	T8747	T8739	punct	", ",cells
R2526	T8748	T8749	det	the,PAAs
R2527	T8749	T8739	conj	PAAs,cells
R2528	T8750	T8751	advmod	abnormally,large
R2529	T8751	T8749	amod	large,PAAs
R253	T2703	T2697	agent	by,caused
R2530	T8752	T8749	amod	sixth,PAAs
R2531	T8753	T8749	cc	and,PAAs
R2532	T8754	T8755	det	the,truncus
R2533	T8755	T8749	conj	truncus,PAAs
R2534	T8756	T8757	amod	strong,staining
R2535	T8757	T8740	dobj	staining,demonstrated
R2536	T8758	T8757	compound	αSMA,staining
R2537	T8759	T8760	punct	(,L
R2538	T8760	T8740	parataxis	L,demonstrated
R2539	T8761	T8760	nmod	Fig.,L
R254	T2704	T2705	det	a,failure
R2540	T8762	T8760	nmod	6H,L
R2541	T8763	T8760	punct	",",L
R2542	T8764	T8760	nmod	J,L
R2543	T8765	T8760	punct	",",L
R2544	T8766	T8760	punct	),L
R2545	T8767	T8740	punct	.,demonstrated
R2546	T8769	T8770	advmod	Recently,showed
R2547	T8771	T8770	punct	", ",showed
R2548	T8772	T8770	nsubj	we,showed
R2549	T8773	T8774	mark	that,display
R255	T2705	T2703	pobj	failure,by
R2550	T8774	T8770	ccomp	display,showed
R2551	T8775	T8776	det	the,mutants
R2552	T8776	T8774	nsubj	mutants,display
R2553	T8777	T8778	npadvmod	NC,specific
R2554	T8778	T8776	amod	specific,mutants
R2555	T8779	T8778	punct	-,specific
R2556	T8780	T8776	prep	of,mutants
R2557	T8781	T8782	det	the,receptor
R2558	T8782	T8780	pobj	receptor,of
R2559	T8783	T8782	amod	related,receptor
R256	T2706	T2705	prep	in,failure
R2560	T8784	T8782	nmod	type,receptor
R2561	T8785	T8784	nummod	I,type
R2562	T8786	T8782	punct	", ",receptor
R2563	T8787	T8782	appos	Alk2,receptor
R2564	T8788	T8774	punct	", ",display
R2565	T8789	T8774	dobj	PTA,display
R2566	T8790	T8791	advmod	as,well
R2567	T8791	T8774	advmod	well,display
R2568	T8792	T8793	punct	[,12
R2569	T8793	T8774	parataxis	12,display
R257	T2707	T2708	amod	normal,migration
R2570	T8794	T8793	punct	],12
R2571	T8795	T8770	punct	.,showed
R2572	T8797	T8798	prep	In,failed
R2573	T8799	T8800	compound	Alk2,Cre
R2574	T8800	T8804	compound	Cre,mutants
R2575	T8801	T8800	punct	/,Cre
R2576	T8802	T8800	compound	Wnt1,Cre
R2577	T8803	T8800	punct	-,Cre
R2578	T8804	T8797	pobj	mutants,In
R2579	T8805	T8798	punct	", ",failed
R258	T2708	T2706	pobj	migration,in
R2580	T8806	T8807	det	the,rotation
R2581	T8807	T8798	nsubj	rotation,failed
R2582	T8808	T8807	prep	of,rotation
R2583	T8809	T8810	det	the,sac
R2584	T8810	T8808	pobj	sac,of
R2585	T8811	T8810	amod	aortic,sac
R2586	T8812	T8810	cc	and,sac
R2587	T8813	T8814	amod	truncal,OFT
R2588	T8814	T8810	conj	OFT,sac
R2589	T8815	T8816	aux	to,occur
R259	T2709	T2708	punct	", ",migration
R2590	T8816	T8798	xcomp	occur,failed
R2591	T8817	T8818	punct	(,6M
R2592	T8818	T8816	parataxis	6M,occur
R2593	T8819	T8818	compound	Fig.,6M
R2594	T8820	T8821	punct	–,R
R2595	T8821	T8818	prep	R,6M
R2596	T8822	T8818	punct	),6M
R2597	T8823	T8824	mark	as,seen
R2598	T8824	T8816	advcl	seen,occur
R2599	T8825	T8824	prep	in,seen
R26	T526	T527	amod	neural,cell
R260	T2710	T2708	conj	differentiation,migration
R2600	T8826	T8827	compound	Alk5,Cre
R2601	T8827	T8831	compound	Cre,mutants
R2602	T8828	T8827	punct	/,Cre
R2603	T8829	T8827	compound	Wnt1,Cre
R2604	T8830	T8827	punct	-,Cre
R2605	T8831	T8825	pobj	mutants,in
R2606	T8832	T8798	punct	.,failed
R2607	T8834	T8835	advmod	However,was
R2608	T8836	T8835	punct	", ",was
R2609	T8837	T8835	prep	in,was
R261	T2711	T2710	cc	or,differentiation
R2610	T8838	T8839	compound	Alk2,mutants
R2611	T8839	T8837	pobj	mutants,in
R2612	T8840	T8841	det	the,pair
R2613	T8841	T8835	nsubj	pair,was
R2614	T8842	T8841	amod	6th,pair
R2615	T8843	T8841	prep	of,pair
R2616	T8844	T8845	det	the,PAAs
R2617	T8845	T8843	pobj	PAAs,of
R2618	T8846	T8847	advmod	grossly,hypoplastic
R2619	T8847	T8835	acomp	hypoplastic,was
R262	T2712	T2710	conj	patterning,differentiation
R2620	T8848	T8835	punct	", ",was
R2621	T8849	T8835	cc	and,was
R2622	T8850	T8851	mark	while,displayed
R2623	T8851	T8859	advcl	displayed,failed
R2624	T8852	T8853	det	the,mutants
R2625	T8853	T8851	nsubj	mutants,displayed
R2626	T8854	T8855	compound	Alk2,Cre
R2627	T8855	T8853	compound	Cre,mutants
R2628	T8856	T8855	punct	/,Cre
R2629	T8857	T8855	compound	Wnt1,Cre
R263	T2713	T2708	prep	of,migration
R2630	T8858	T8855	punct	-,Cre
R2631	T8859	T8835	conj	failed,was
R2632	T8860	T8861	det	a,amount
R2633	T8861	T8851	dobj	amount,displayed
R2634	T8862	T8861	amod	noticeable,amount
R2635	T8863	T8861	prep	of,amount
R2636	T8864	T8865	amod	septal,tissue
R2637	T8865	T8863	pobj	tissue,of
R2638	T8866	T8861	prep	between,amount
R2639	T8867	T8868	det	the,PAAs
R264	T2714	T2715	det	the,crest
R2640	T8868	T8866	pobj	PAAs,between
R2641	T8869	T8868	nmod	4th,PAAs
R2642	T8870	T8869	cc	and,4th
R2643	T8871	T8869	conj	6th,4th
R2644	T8872	T8873	punct	(,N
R2645	T8873	T8851	parataxis	N,displayed
R2646	T8874	T8873	nmod	Fig.,N
R2647	T8875	T8873	nmod	6M,N
R2648	T8876	T8873	punct	",",N
R2649	T8877	T8873	punct	),N
R265	T2715	T2713	pobj	crest,of
R2650	T8878	T8859	punct	", ",failed
R2651	T8879	T8880	det	the,mesenchyme
R2652	T8880	T8859	nsubj	mesenchyme,failed
R2653	T8881	T8880	amod	condensed,mesenchyme
R2654	T8882	T8880	amod	septal,mesenchyme
R2655	T8883	T8880	acl	lacking,mesenchyme
R2656	T8884	T8883	dobj	Alk2,lacking
R2657	T8885	T8886	aux	to,extend
R2658	T8886	T8859	xcomp	extend,failed
R2659	T8887	T8888	det	the,prongs
R266	T2716	T2715	amod	cardiac,crest
R2660	T8888	T8886	dobj	prongs,extend
R2661	T8889	T8886	prep	into,extend
R2662	T8890	T8891	det	the,cushions
R2663	T8891	T8889	pobj	cushions,into
R2664	T8892	T8891	amod	truncal,cushions
R2665	T8893	T8886	cc	and,extend
R2666	T8894	T8895	aux	to,form
R2667	T8895	T8886	conj	form,extend
R2668	T8896	T8897	det	the,septum
R2669	T8897	T8895	dobj	septum,form
R267	T2717	T2715	amod	neural,crest
R2670	T8898	T8897	compound	AP,septum
R2671	T8899	T8859	punct	.,failed
R2672	T8901	T8902	advmod	Concurrently,losing
R2673	T8903	T8902	punct	", ",losing
R2674	T8904	T8905	det	the,PAAs
R2675	T8905	T8902	nsubj	PAAs,losing
R2676	T8906	T8905	amod	6th,PAAs
R2677	T8907	T8902	aux	were,losing
R2678	T8908	T8909	poss	their,patency
R2679	T8909	T8902	dobj	patency,losing
R268	T2718	T2715	punct	(,crest
R2680	T8910	T8902	punct	", ",losing
R2681	T8911	T8912	dep	which,contributed
R2682	T8912	T8902	advcl	contributed,losing
R2683	T8913	T8912	aux	may,contributed
R2684	T8914	T8912	aux	have,contributed
R2685	T8915	T8912	advmod	further,contributed
R2686	T8916	T8912	prep	to,contributed
R2687	T8917	T8918	det	the,septation
R2688	T8918	T8916	pobj	septation,to
R2689	T8919	T8918	amod	failed,septation
R269	T2719	T2715	appos	CNC,crest
R2690	T8920	T8918	compound	AP,septation
R2691	T8921	T8922	punct	(,Q
R2692	T8922	T8902	parataxis	Q,losing
R2693	T8923	T8922	nmod	Fig.,Q
R2694	T8924	T8922	nmod	6M,Q
R2695	T8925	T8922	punct	",",Q
R2696	T8926	T8922	nmod	O,Q
R2697	T8927	T8922	punct	",",Q
R2698	T8928	T8922	punct	),Q
R2699	T8929	T8902	punct	.,losing
R27	T527	T524	compound	cell,apoptosis
R270	T2720	T2697	punct	),caused
R2700	T8931	T8932	mark	While,managed
R2701	T8932	T8934	advcl	managed,appeared
R2702	T8933	T8932	nsubj	CNCCs,managed
R2703	T8935	T8936	aux	to,migrate
R2704	T8936	T8932	xcomp	migrate,managed
R2705	T8937	T8936	prep	to,migrate
R2706	T8938	T8939	det	the,sac
R2707	T8939	T8937	pobj	sac,to
R2708	T8940	T8939	amod	aortic,sac
R2709	T8941	T8939	cc	and,sac
R271	T2721	T2697	punct	.,caused
R2710	T8942	T8943	det	the,level
R2711	T8943	T8939	conj	level,sac
R2712	T8944	T8943	amod	truncal,level
R2713	T8945	T8943	compound	cushion,level
R2714	T8946	T8947	punct	(,R
R2715	T8947	T8936	parataxis	R,migrate
R2716	T8948	T8947	nmod	Fig.,R
R2717	T8949	T8947	nmod	6N,R
R2718	T8950	T8947	punct	",",R
R2719	T8951	T8947	nmod	P,R
R272	T2723	T2724	det	This,subset
R2720	T8952	T8947	punct	",",R
R2721	T8953	T8947	punct	),R
R2722	T8954	T8934	punct	", ",appeared
R2723	T8955	T8934	nsubj	immunostaining,appeared
R2724	T8956	T8955	prep	for,immunostaining
R2725	T8957	T8956	pobj	αSMA,for
R2726	T8958	T8959	advmod	much,weaker
R2727	T8959	T8934	oprd	weaker,appeared
R2728	T8960	T8961	advmod	when,compared
R2729	T8961	T8934	advcl	compared,appeared
R273	T2724	T2725	nsubj	subset,forms
R2730	T8962	T8961	prep	to,compared
R2731	T8963	T8962	pobj	controls,to
R2732	T8964	T8963	cc	and,controls
R2733	T8965	T8966	compound	Alk5,mutants
R2734	T8966	T8963	conj	mutants,controls
R2735	T8967	T8934	punct	", ",appeared
R2736	T8968	T8934	advcl	implying,appeared
R2737	T8969	T8970	mark	that,involved
R2738	T8970	T8968	ccomp	involved,implying
R2739	T8971	T8972	npadvmod	ALK2,mediated
R274	T2726	T2724	prep	of,subset
R2740	T8972	T8974	amod	mediated,signaling
R2741	T8973	T8972	punct	-,mediated
R2742	T8974	T8970	nsubjpass	signaling,involved
R2743	T8975	T8970	auxpass	is,involved
R2744	T8976	T8970	prep	in,involved
R2745	T8977	T8978	amod	smooth,muscle
R2746	T8978	T8979	compound	muscle,differentiation
R2747	T8979	T8976	pobj	differentiation,in
R2748	T8980	T8979	compound	cell,differentiation
R2749	T8981	T8982	mark	as,suggested
R275	T2727	T2728	amod	pluripotent,crest
R2750	T8982	T8970	advcl	suggested,involved
R2751	T8983	T8982	advmod	previously,suggested
R2752	T8984	T8985	punct	[,12
R2753	T8985	T8970	parataxis	12,involved
R2754	T8986	T8985	punct	],12
R2755	T8987	T8934	punct	.,appeared
R2756	T8989	T8990	aux	To,conclude
R2757	T8990	T8991	advcl	conclude,appear
R2758	T8992	T8991	punct	", ",appear
R2759	T8993	T8994	mark	while,demonstrate
R276	T2728	T2730	compound	crest,cells
R2760	T8994	T8991	advcl	demonstrate,appear
R2761	T8995	T8996	preconj	both,Alk2
R2762	T8996	T8997	nmod	Alk2,mutants
R2763	T8997	T8994	nsubj	mutants,demonstrate
R2764	T8998	T8996	cc	and,Alk2
R2765	T8999	T8996	conj	Alk5,Alk2
R2766	T9000	T9001	det	a,failure
R2767	T9001	T8994	dobj	failure,demonstrate
R2768	T9002	T9001	prep	in,failure
R2769	T9003	T9004	preconj	both,rotation
R277	T2729	T2728	amod	neural,crest
R2770	T9004	T9002	pobj	rotation,in
R2771	T9005	T9004	det	the,rotation
R2772	T9006	T9004	prep	of,rotation
R2773	T9007	T9008	det	the,sac
R2774	T9008	T9006	pobj	sac,of
R2775	T9009	T9008	amod	aortic,sac
R2776	T9010	T9004	cc	and,rotation
R2777	T9011	T9012	det	the,OFT
R2778	T9012	T9004	conj	OFT,rotation
R2779	T9013	T9012	amod	truncal,OFT
R278	T2730	T2726	pobj	cells,of
R2780	T9014	T9002	punct	", ",in
R2781	T9015	T9002	cc	and,in
R2782	T9016	T9002	conj	in,in
R2783	T9017	T9018	det	the,formation
R2784	T9018	T9016	pobj	formation,in
R2785	T9019	T9018	prep	of,formation
R2786	T9020	T9021	det	the,septum
R2787	T9021	T9019	pobj	septum,of
R2788	T9022	T9021	compound	AP,septum
R2789	T9023	T8991	punct	", ",appear
R279	T2837	T2838	aux	to,form
R2790	T9024	T9025	det	the,mechanisms
R2791	T9025	T8991	nsubj	mechanisms,appear
R2792	T9026	T9025	amod	pathogenetic,mechanisms
R2793	T9027	T9025	prep	behind,mechanisms
R2794	T9028	T9029	det	these,defects
R2795	T9029	T9027	pobj	defects,behind
R2796	T9030	T9031	advmod	remarkably,different
R2797	T9031	T8991	oprd	different,appear
R2798	T9032	T8991	punct	.,appear
R28	T528	T527	compound	crest,cell
R280	T2838	T2831	advcl	form,migrate
R2809	T9837	T9838	compound	Alk5,Cre
R281	T2839	T2840	det	the,septum
R2810	T9838	T9842	compound	Cre,mutants
R2811	T9839	T9838	punct	/,Cre
R2812	T9840	T9838	compound	Wnt1,Cre
R2813	T9841	T9838	punct	-,Cre
R2814	T9842	T9843	nsubj	mutants,display
R2815	T9844	T9845	amod	increased,apoptosis
R2816	T9845	T9843	dobj	apoptosis,display
R2817	T9846	T9845	prep	of,apoptosis
R2818	T9847	T9848	amod	post-migratory,cells
R2819	T9848	T9846	pobj	cells,of
R282	T2731	T2730	compound	stem,cells
R2820	T9849	T9850	amod	neural,crest
R2821	T9850	T9848	compound	crest,cells
R2822	T9852	T9853	mark	As,described
R2823	T9853	T9854	advcl	described,displayed
R2824	T9855	T9853	advmod	above,described
R2825	T9856	T9854	punct	", ",displayed
R2826	T9857	T9858	compound	Alk5,Cre
R2827	T9858	T9862	compound	Cre,mutants
R2828	T9859	T9858	punct	/,Cre
R2829	T9860	T9858	compound	Wnt1,Cre
R283	T2840	T2838	dobj	septum,form
R2830	T9861	T9858	punct	-,Cre
R2831	T9862	T9854	nsubj	mutants,displayed
R2832	T9863	T9864	det	an,amount
R2833	T9864	T9854	dobj	amount,displayed
R2834	T9865	T9864	amod	inadequate,amount
R2835	T9866	T9864	prep	of,amount
R2836	T9867	T9868	npadvmod	AP,septal
R2837	T9868	T9870	amod	septal,tissue
R2838	T9869	T9868	punct	-,septal
R2839	T9870	T9866	pobj	tissue,of
R284	T2841	T2842	amod	aortico,pulmonary
R2840	T9871	T9864	prep	in,amount
R2841	T9872	T9873	det	the,base
R2842	T9873	T9871	pobj	base,in
R2843	T9874	T9873	prep	of,base
R2844	T9875	T9876	det	the,sac
R2845	T9876	T9874	pobj	sac,of
R2846	T9877	T9876	amod	aortic,sac
R2847	T9878	T9873	prep	between,base
R2848	T9879	T9880	det	the,origins
R2849	T9880	T9878	pobj	origins,between
R285	T2732	T2725	prep	in,forms
R2850	T9881	T9880	prep	of,origins
R2851	T9882	T9883	amod	4th,PAAs
R2852	T9883	T9881	pobj	PAAs,of
R2853	T9884	T9882	cc	and,4th
R2854	T9885	T9882	conj	6th,4th
R2855	T9886	T9854	punct	.,displayed
R2856	T9888	T9889	aux	To,analyze
R2857	T9889	T9890	advcl	analyze,used
R2858	T9891	T9892	mark	whether,resulted
R2859	T9892	T9889	ccomp	resulted,analyze
R286	T2733	T2734	det	the,aspect
R2860	T9893	T9894	det	this,phenotype
R2861	T9894	T9892	nsubj	phenotype,resulted
R2862	T9895	T9896	preconj	either,from
R2863	T9896	T9892	prep	from,resulted
R2864	T9897	T9898	amod	defective,proliferation
R2865	T9898	T9896	pobj	proliferation,from
R2866	T9899	T9898	compound	CNCC,proliferation
R2867	T9900	T9898	cc	or,proliferation
R2868	T9901	T9902	amod	inappropriate,apoptosis
R2869	T9902	T9898	conj	apoptosis,proliferation
R287	T2842	T2840	amod	pulmonary,septum
R2870	T9903	T9890	punct	", ",used
R2871	T9904	T9890	nsubj	we,used
R2872	T9905	T9906	nmod	BrdU,staining
R2873	T9906	T9890	dobj	staining,used
R2874	T9907	T9905	cc	and,BrdU
R2875	T9908	T9905	conj	TUNEL,BrdU
R2876	T9909	T9890	punct	", ",used
R2877	T9910	T9890	advmod	respectively,used
R2878	T9911	T9890	punct	.,used
R2879	T9913	T9914	mark	While,affected
R288	T2734	T2732	pobj	aspect,in
R2880	T9914	T9919	advcl	affected,detect
R2881	T9915	T9916	compound	CNCC,proliferation
R2882	T9916	T9914	nsubjpass	proliferation,affected
R2883	T9917	T9914	auxpass	was,affected
R2884	T9918	T9914	neg	not,affected
R2885	T9920	T9914	prep	in,affected
R2886	T9921	T9922	compound	Alk5,mutants
R2887	T9922	T9920	pobj	mutants,in
R2888	T9923	T9924	punct	(,shown
R2889	T9924	T9914	parataxis	shown,affected
R289	T2735	T2734	amod	dorsal,aspect
R2890	T9925	T9924	nsubj	data,shown
R2891	T9926	T9924	neg	not,shown
R2892	T9927	T9924	punct	),shown
R2893	T9928	T9919	punct	", ",detect
R2894	T9929	T9919	nsubj	we,detect
R2895	T9930	T9919	aux	could,detect
R2896	T9931	T9932	det	a,increase
R2897	T9932	T9919	dobj	increase,detect
R2898	T9933	T9932	amod	dramatic,increase
R2899	T9934	T9932	prep	in,increase
R29	T529	T524	cc	and,apoptosis
R290	T2843	T2842	punct	-,pulmonary
R2900	T9935	T9936	det	the,number
R2901	T9936	T9934	pobj	number,in
R2902	T9937	T9936	prep	of,number
R2903	T9938	T9939	npadvmod	TUNEL,positive
R2904	T9939	T9940	amod	positive,cells
R2905	T9940	T9937	pobj	cells,of
R2906	T9941	T9932	prep	in,increase
R2907	T9942	T9941	pobj	tissues,in
R2908	T9943	T9942	acl	surrounding,tissues
R2909	T9944	T9945	det	the,sac
R291	T2736	T2734	prep	of,aspect
R2910	T9945	T9943	dobj	sac,surrounding
R2911	T9946	T9945	amod	aortic,sac
R2912	T9947	T9942	prep	including,tissues
R2913	T9948	T9949	det	the,site
R2914	T9949	T9947	pobj	site,including
R2915	T9950	T9951	advmod	where,forms
R2916	T9951	T9949	relcl	forms,site
R2917	T9952	T9953	det	the,septum
R2918	T9953	T9951	nsubj	septum,forms
R2919	T9954	T9953	compound	AP,septum
R292	T2737	T2738	det	the,tube
R2920	T9955	T9953	punct	-,septum
R2921	T9956	T9957	punct	(,7A
R2922	T9957	T9951	parataxis	7A,forms
R2923	T9958	T9957	compound	Fig.,7A
R2924	T9959	T9960	punct	–,C
R2925	T9960	T9957	prep	C,7A
R2926	T9961	T9957	punct	),7A
R2927	T9962	T9919	punct	.,detect
R2928	T9964	T9965	amod	Dual,staining
R2929	T9965	T9966	nsubj	staining,demonstrated
R293	T2738	T2736	pobj	tube,of
R2930	T9966	T9973	ccomp	demonstrated,was
R2931	T9967	T9965	prep	for,staining
R2932	T9968	T9969	npadvmod	lacZ,positive
R2933	T9969	T9972	amod	positive,cells
R2934	T9970	T9968	cc	and,lacZ
R2935	T9971	T9968	conj	TUNEL,lacZ
R2936	T9972	T9967	pobj	cells,for
R2937	T9974	T9975	mark	that,were
R2938	T9975	T9966	ccomp	were,demonstrated
R2939	T9976	T9977	det	these,cells
R294	T2844	T2840	punct	;,septum
R2940	T9977	T9975	nsubj	cells,were
R2941	T9978	T9979	amod	postmigratory,CNCCs
R2942	T9979	T9975	attr	CNCCs,were
R2943	T9980	T9973	punct	;,was
R2944	T9981	T9982	det	this,phenotype
R2945	T9982	T9973	nsubj	phenotype,was
R2946	T9983	T9973	advmod	already,was
R2947	T9984	T9985	advmod	clearly,detectable
R2948	T9985	T9973	acomp	detectable,was
R2949	T9986	T9973	prep	at,was
R295	T2739	T2738	amod	neural,tube
R2950	T9987	T9986	pobj	E10.5,at
R2951	T9988	T9973	punct	.,was
R2952	T9990	T9991	det	These,results
R2953	T9991	T9992	nsubjpass	results,confirmed
R2954	T9993	T9992	auxpass	were,confirmed
R2955	T9994	T9992	prep	by,confirmed
R2956	T9995	T9994	pcomp	using,by
R2957	T9996	T9995	dobj	immunostaining,using
R2958	T9997	T9995	prep	for,using
R2959	T9998	T9999	amod	cleaved,caspase
R296	T2740	T2725	prep	at,forms
R2960	T9999	T9997	pobj	caspase,for
R2961	T10000	T9999	punct	-,caspase
R2962	T10001	T9999	nummod	3,caspase
R2963	T10002	T9999	punct	", ",caspase
R2964	T10003	T10004	det	another,marker
R2965	T10004	T9999	appos	marker,caspase
R2966	T10005	T10004	prep	for,marker
R2967	T10006	T10005	pobj	apoptosis,for
R2968	T10007	T10008	punct	(,J
R2969	T10008	T9997	parataxis	J,for
R297	T2741	T2742	det	the,level
R2970	T10009	T10008	nmod	Fig.,J
R2971	T10010	T10008	nmod	7I,J
R2972	T10011	T10008	punct	",",J
R2973	T10012	T10008	punct	),J
R2974	T10013	T9992	punct	.,confirmed
R2975	T10015	T10016	prep	In,found
R2976	T10017	T10018	det	the,chick
R2977	T10018	T10015	pobj	chick,In
R2978	T10019	T10016	punct	", ",found
R2979	T10020	T10021	amod	apoptotic,cells
R298	T2845	T2846	det	a,structure
R2980	T10021	T10016	nsubjpass	cells,found
R2981	T10022	T10023	amod	neural,crest
R2982	T10023	T10024	npadvmod	crest,derived
R2983	T10024	T10021	amod	derived,cells
R2984	T10025	T10024	punct	-,derived
R2985	T10026	T10016	aux	have,found
R2986	T10027	T10016	advmod	also,found
R2987	T10028	T10016	auxpass	been,found
R2988	T10029	T10016	prep	at,found
R2989	T10030	T10031	det	the,sites
R299	T2742	T2740	pobj	level,at
R2990	T10031	T10029	pobj	sites,at
R2991	T10032	T10031	punct	", ",sites
R2992	T10033	T10034	advmod	where,penetrate
R2993	T10034	T10031	relcl	penetrate,sites
R2994	T10035	T10036	det	the,prongs
R2995	T10036	T10034	nsubj	prongs,penetrate
R2996	T10037	T10036	prep	of,prongs
R2997	T10038	T10039	det	the,septum
R2998	T10039	T10037	pobj	septum,of
R2999	T10040	T10039	compound	AP,septum
R30	T530	T531	amod	severe,defects
R300	T2743	T2742	prep	of,level
R3000	T10041	T10034	prep	into,penetrate
R3001	T10042	T10043	det	the,mesenchyme
R3002	T10043	T10041	pobj	mesenchyme,into
R3003	T10044	T10043	compound	OFT,mesenchyme
R3004	T10045	T10043	compound	cushion,mesenchyme
R3005	T10046	T10047	punct	[,26
R3006	T10047	T10016	parataxis	26,found
R3007	T10048	T10047	nummod	25,26
R3008	T10049	T10047	punct	",",26
R3009	T10050	T10047	punct	],26
R301	T2846	T2840	appos	structure,septum
R3010	T10051	T10016	punct	.,found
R3011	T10053	T10054	advmod	Thus,compared
R3012	T10055	T10054	punct	", ",compared
R3013	T10056	T10054	nsubj	we,compared
R3014	T10057	T10058	compound	apoptosis,patterns
R3015	T10058	T10054	dobj	patterns,compared
R3016	T10059	T10060	advmod	also,on
R3017	T10060	T10054	prep	on,compared
R3018	T10061	T10062	det	the,level
R3019	T10062	T10060	pobj	level,on
R302	T2744	T2745	det	the,placode
R3020	T10063	T10064	advmod	more,proximal
R3021	T10064	T10062	amod	proximal,level
R3022	T10065	T10054	punct	", ",compared
R3023	T10066	T10054	cc	but,compared
R3024	T10067	T10054	conj	found,compared
R3025	T10068	T10069	det	no,differences
R3026	T10069	T10067	dobj	differences,found
R3027	T10070	T10069	amod	detectable,differences
R3028	T10071	T10069	prep	at,differences
R3029	T10072	T10071	pobj	E11.0,at
R303	T2745	T2743	pobj	placode,of
R3030	T10073	T10069	prep	between,differences
R3031	T10074	T10075	compound	Alk5,Cre
R3032	T10075	T10079	compound	Cre,mutants
R3033	T10076	T10075	punct	/,Cre
R3034	T10077	T10075	compound	Wnt1,Cre
R3035	T10078	T10075	punct	-,Cre
R3036	T10079	T10073	pobj	mutants,between
R3037	T10080	T10079	cc	and,mutants
R3038	T10081	T10079	conj	controls,mutants
R3039	T10082	T10083	punct	(,E
R304	T2746	T2745	amod	mid-otic,placode
R3040	T10083	T10067	parataxis	E,found
R3041	T10084	T10083	nmod	Fig.,E
R3042	T10085	T10083	nmod	7D,E
R3043	T10086	T10083	punct	",",E
R3044	T10087	T10083	punct	),E
R3045	T10088	T10054	punct	.,compared
R3046	T10090	T10091	advcl	Unlike,is
R3047	T10092	T10090	prep	in,Unlike
R3048	T10093	T10094	compound	Alk5,Cre
R3049	T10094	T10098	compound	Cre,embryos
R305	T2847	T2846	amod	vital,structure
R3050	T10095	T10094	punct	/,Cre
R3051	T10096	T10094	compound	Wnt1,Cre
R3052	T10097	T10094	punct	-,Cre
R3053	T10098	T10092	pobj	embryos,in
R3054	T10099	T10098	compound	mutant,embryos
R3055	T10100	T10091	punct	", ",is
R3056	T10101	T10102	amod	increased,apoptosis
R3057	T10102	T10091	nsubj	apoptosis,is
R3058	T10103	T10102	prep	of,apoptosis
R3059	T10104	T10105	npadvmod	NC,derived
R306	T2747	T2742	prep	to,level
R3060	T10105	T10107	amod	derived,cells
R3061	T10106	T10105	punct	-,derived
R3062	T10107	T10103	pobj	cells,of
R3063	T10108	T10091	neg	not,is
R3064	T10109	T10091	acomp	responsible,is
R3065	T10110	T10109	prep	for,responsible
R3066	T10111	T10112	det	the,defects
R3067	T10112	T10110	pobj	defects,for
R3068	T10113	T10112	amod	observed,defects
R3069	T10114	T10112	prep	in,defects
R307	T2748	T2749	det	the,somite
R3070	T10115	T10116	det	the,septation
R3071	T10116	T10114	pobj	septation,in
R3072	T10117	T10116	compound	OFT,septation
R3073	T10118	T10116	prep	in,septation
R3074	T10119	T10120	amod	corresponding,mutants
R3075	T10120	T10118	pobj	mutants,in
R3076	T10121	T10120	compound	Alk2,mutants
R3077	T10122	T10123	punct	(,E
R3078	T10123	T10091	parataxis	E,is
R3079	T10124	T10123	nmod	Fig.,E
R308	T2749	T2747	pobj	somite,to
R3080	T10125	T10123	nmod	7C,E
R3081	T10126	T10123	punct	",",E
R3082	T10127	T10123	punct	),E
R3083	T10128	T10091	punct	.,is
R3084	T10130	T10131	aux	To,conclude
R3085	T10131	T10132	advcl	conclude,suggest
R3086	T10133	T10132	punct	", ",suggest
R3087	T10134	T10135	poss	our,results
R3088	T10135	T10132	nsubj	results,suggest
R3089	T10136	T10137	mark	that,coincides
R309	T2848	T2846	punct	", ",structure
R3090	T10137	T10132	ccomp	coincides,suggest
R3091	T10138	T10137	prep	in,coincides
R3092	T10139	T10140	compound	Alk5,Cre
R3093	T10140	T10144	compound	Cre,mutants
R3094	T10141	T10140	punct	/,Cre
R3095	T10142	T10140	compound	Wnt1,Cre
R3096	T10143	T10140	punct	-,Cre
R3097	T10144	T10138	pobj	mutants,in
R3098	T10145	T10146	det	a,increase
R3099	T10146	T10137	nsubj	increase,coincides
R31	T531	T524	conj	defects,apoptosis
R310	T2750	T2749	amod	third,somite
R3100	T10147	T10146	amod	noticeable,increase
R3101	T10148	T10146	prep	in,increase
R3102	T10149	T10148	pobj	apoptosis,in
R3103	T10150	T10137	prep	with,coincides
R3104	T10151	T10152	det	the,patterning
R3105	T10152	T10150	pobj	patterning,with
R3106	T10153	T10152	amod	abnormal,patterning
R3107	T10154	T10152	prep	of,patterning
R3108	T10155	T10156	det	the,PAAs
R3109	T10156	T10154	pobj	PAAs,of
R311	T2751	T2752	punct	[,1
R3110	T10157	T10156	cc	and,PAAs
R3111	T10158	T10159	det	the,sac
R3112	T10159	T10156	conj	sac,PAAs
R3113	T10160	T10159	amod	aortic,sac
R3114	T10161	T10150	punct	", ",with
R3115	T10162	T10150	cc	and,with
R3116	T10163	T10150	conj	with,with
R3117	T10164	T10165	det	the,septation
R3118	T10165	T10163	pobj	septation,with
R3119	T10166	T10165	amod	failed,septation
R312	T2752	T2725	parataxis	1,forms
R3120	T10167	T10165	compound	AP,septation
R3121	T10168	T10165	punct	-,septation
R3122	T10169	T10132	punct	.,suggest
R3123	T10171	T10172	det	These,data
R3124	T10172	T10173	nsubj	data,support
R3125	T10174	T10175	det	a,role
R3126	T10175	T10173	dobj	role,support
R3127	T10176	T10175	amod	specific,role
R3128	T10177	T10175	prep	for,role
R3129	T10178	T10179	compound	ALK5,signaling
R313	T2753	T2752	punct	],1
R3130	T10179	T10177	pobj	signaling,for
R3131	T10180	T10179	punct	", ",signaling
R3132	T10181	T10182	preconj	either,directly
R3133	T10182	T10179	advmod	directly,signaling
R3134	T10183	T10182	cc	or,directly
R3135	T10184	T10182	conj	indirectly,directly
R3136	T10185	T10175	punct	", ",role
R3137	T10186	T10175	prep	in,role
R3138	T10187	T10188	compound	CNCC,survival
R3139	T10188	T10186	pobj	survival,in
R314	T2754	T2725	punct	.,forms
R3140	T10189	T10173	punct	", ",support
R3141	T10190	T10191	mark	since,seen
R3142	T10191	T10173	advcl	seen,support
R3143	T10192	T10193	det	a,apoptosis
R3144	T10193	T10191	nsubjpass	apoptosis,seen
R3145	T10194	T10193	amod	similar,apoptosis
R3146	T10195	T10193	prep	of,apoptosis
R3147	T10196	T10197	npadvmod	NC,derived
R3148	T10197	T10199	amod	derived,cells
R3149	T10198	T10197	punct	-,derived
R315	T2849	T2850	dep	which,separates
R3150	T10199	T10195	pobj	cells,of
R3151	T10200	T10191	auxpass	is,seen
R3152	T10201	T10191	neg	not,seen
R3153	T10202	T10191	prep	in,seen
R3154	T10203	T10204	compound	Tgfbr2,Cre
R3155	T10204	T10208	compound	Cre,mutants
R3156	T10205	T10204	punct	/,Cre
R3157	T10206	T10204	compound	Wnt1,Cre
R3158	T10207	T10204	punct	-,Cre
R3159	T10208	T10202	pobj	mutants,in
R316	T2756	T2757	advmod	Subsequently,delaminate
R3160	T10209	T10210	punct	[,9
R3161	T10210	T10191	parataxis	9,seen
R3162	T10211	T10210	nummod	8,9
R3163	T10212	T10210	punct	",",9
R3164	T10213	T10210	punct	],9
R3165	T10214	T10173	punct	.,support
R3168	T10806	T10807	amod	Pharyngeal,organs
R3169	T10807	T10808	nsubj	organs,fail
R317	T2758	T2759	amod	cardiac,cells
R3170	T10809	T10810	aux	to,migrate
R3171	T10810	T10808	xcomp	migrate,fail
R3172	T10811	T10810	prep	in,migrate
R3173	T10812	T10813	compound	Alk5,Cre
R3174	T10813	T10817	compound	Cre,mutants
R3175	T10814	T10813	punct	/,Cre
R3176	T10815	T10813	compound	Wnt1,Cre
R3177	T10816	T10813	punct	-,Cre
R3178	T10817	T10811	pobj	mutants,in
R3179	T10819	T10820	prep	In,was
R318	T2850	T2846	relcl	separates,structure
R3180	T10821	T10819	pobj	addition,In
R3181	T10822	T10821	prep	to,addition
R3182	T10823	T10824	det	the,OFT
R3183	T10824	T10822	pobj	OFT,to
R3184	T10825	T10824	amod	cardiac,OFT
R3185	T10826	T10820	punct	", ",was
R3186	T10827	T10820	nsubj	development,was
R3187	T10828	T10827	prep	of,development
R3188	T10829	T10830	amod	pharyngeal,organs
R3189	T10830	T10828	pobj	organs,of
R319	T2759	T2757	nsubj	cells,delaminate
R3190	T10831	T10827	punct	", ",development
R3191	T10832	T10833	advmod	i.e.,glands
R3192	T10833	T10827	appos	glands,development
R3193	T10834	T10833	punct	", ",glands
R3194	T10835	T10833	det	the,glands
R3195	T10836	T10833	amod	parathyroid,glands
R3196	T10837	T10833	cc	and,glands
R3197	T10838	T10839	det	the,thymus
R3198	T10839	T10833	conj	thymus,glands
R3199	T10840	T10820	advmod	also,was
R32	T532	T533	compound	outflow,tract
R320	T2760	T2761	amod	neural,crest
R3200	T10841	T10820	acomp	abnormal,was
R3201	T10842	T10820	prep	in,was
R3202	T10843	T10844	compound	Alk5,Cre
R3203	T10844	T10848	compound	Cre,mutants
R3204	T10845	T10844	punct	/,Cre
R3205	T10846	T10844	compound	Wnt1,Cre
R3206	T10847	T10844	punct	-,Cre
R3207	T10848	T10842	pobj	mutants,in
R3208	T10849	T10850	punct	(,see
R3209	T10850	T10820	parataxis	see,was
R321	T2761	T2759	compound	crest,cells
R3210	T10851	T10852	nmod	Figs.,1
R3211	T10852	T10850	dobj	1,see
R3212	T10853	T10852	cc	and,1
R3213	T10854	T10852	conj	8,1
R3214	T10855	T10850	punct	),see
R3215	T10856	T10820	punct	.,was
R3216	T10858	T10859	advmod	Normally,develops
R3217	T10860	T10861	det	the,thymus
R3218	T10861	T10859	nsubj	thymus,develops
R3219	T10862	T10859	prep	from,develops
R322	T2851	T2852	det	the,trunk
R3220	T10863	T10864	det	the,endoderm
R3221	T10864	T10862	pobj	endoderm,from
R3222	T10865	T10864	amod	third,endoderm
R3223	T10866	T10864	amod	pharyngeal,endoderm
R3224	T10867	T10864	compound	pouch,endoderm
R3225	T10868	T10859	cc	and,develops
R3226	T10869	T10859	conj	migrates,develops
R3227	T10870	T10869	advmod	caudally,migrates
R3228	T10871	T10869	prep	to,migrates
R3229	T10872	T10873	poss	its,location
R323	T2762	T2759	punct	(,cells
R3230	T10873	T10871	pobj	location,to
R3231	T10874	T10873	amod	final,location
R3232	T10875	T10873	prep	in,location
R3233	T10876	T10877	det	the,mediastinum
R3234	T10877	T10875	pobj	mediastinum,in
R3235	T10878	T10877	amod	superior,mediastinum
R3236	T10879	T10880	mark	as,seen
R3237	T10880	T10869	advcl	seen,migrates
R3238	T10881	T10880	prep	in,seen
R3239	T10882	T10881	pobj	controls,in
R324	T2852	T2850	dobj	trunk,separates
R3240	T10883	T10869	prep	at,migrates
R3241	T10884	T10883	pobj	E14,at
R3242	T10885	T10886	punct	(,B
R3243	T10886	T10869	parataxis	B,migrates
R3244	T10887	T10886	nmod	Fig.,B
R3245	T10888	T10886	nmod	8A,B
R3246	T10889	T10886	punct	",",B
R3247	T10890	T10886	punct	),B
R3248	T10891	T10859	punct	.,develops
R3249	T10893	T10894	prep	In,failed
R325	T2763	T2759	appos	CNCCs,cells
R3250	T10895	T10893	pobj	contrast,In
R3251	T10896	T10894	punct	", ",failed
R3252	T10897	T10898	det	the,primordia
R3253	T10898	T10894	nsubj	primordia,failed
R3254	T10899	T10898	amod	thymic,primordia
R3255	T10900	T10898	prep	of,primordia
R3256	T10901	T10902	det	the,littermates
R3257	T10902	T10900	pobj	littermates,of
R3258	T10903	T10902	compound	Alk5,littermates
R3259	T10904	T10902	compound	mutant,littermates
R326	T2764	T2757	punct	),delaminate
R3260	T10905	T10906	aux	to,descend
R3261	T10906	T10894	xcomp	descend,failed
R3262	T10907	T10906	advmod	caudally,descend
R3263	T10908	T10894	punct	", ",failed
R3264	T10909	T10894	cc	and,failed
R3265	T10910	T10911	auxpass	were,located
R3266	T10911	T10894	conj	located,failed
R3267	T10912	T10911	advmod	bilaterally,located
R3268	T10913	T10911	prep	in,located
R3269	T10914	T10915	det	the,region
R327	T2765	T2757	punct	", ",delaminate
R3270	T10915	T10913	pobj	region,in
R3271	T10916	T10915	compound	neck,region
R3272	T10917	T10915	punct	", ",region
R3273	T10918	T10919	advmod	where,surrounded
R3274	T10919	T10915	relcl	surrounded,region
R3275	T10920	T10919	nsubjpass	they,surrounded
R3276	T10921	T10919	auxpass	were,surrounded
R3277	T10922	T10919	agent	by,surrounded
R3278	T10923	T10924	amod	neural,crest
R3279	T10924	T10925	npadvmod	crest,derived
R328	T2766	T2757	conj	undergo,delaminate
R3280	T10925	T10927	amod	derived,mesenchyme
R3281	T10926	T10925	punct	-,derived
R3282	T10927	T10922	pobj	mesenchyme,by
R3283	T10928	T10929	punct	(,E
R3284	T10929	T10911	parataxis	E,located
R3285	T10930	T10929	nmod	Fig.,E
R3286	T10931	T10929	nmod	8D,E
R3287	T10932	T10929	punct	",",E
R3288	T10933	T10929	punct	),E
R3289	T10934	T10894	punct	.,failed
R329	T2853	T2852	amod	pulmonary,trunk
R3290	T10936	T10937	det	The,assay
R3291	T10937	T10940	nsubj	assay,demonstrated
R3292	T10938	T10939	compound	fate,determination
R3293	T10939	T10937	compound	determination,assay
R3294	T10941	T10942	mark	that,populated
R3295	T10942	T10940	ccomp	populated,demonstrated
R3296	T10943	T10944	det	the,primordia
R3297	T10944	T10942	nsubjpass	primordia,populated
R3298	T10945	T10944	amod	thymic,primordia
R3299	T10946	T10942	auxpass	were,populated
R33	T533	T531	compound	tract,defects
R330	T2767	T2768	det	a,transformation
R3300	T10947	T10942	advmod	equally,populated
R3301	T10948	T10942	agent	by,populated
R3302	T10949	T10948	pobj	NCCs,by
R3303	T10950	T10951	preconj	both,in
R3304	T10951	T10942	prep	in,populated
R3305	T10952	T10951	pobj	controls,in
R3306	T10953	T10952	cc	and,controls
R3307	T10954	T10955	compound	Alk5,mutants
R3308	T10955	T10952	conj	mutants,controls
R3309	T10956	T10957	punct	(,E
R331	T2768	T2766	dobj	transformation,undergo
R3310	T10957	T10942	parataxis	E,populated
R3311	T10958	T10957	nmod	Fig.,E
R3312	T10959	T10957	nmod	8B,E
R3313	T10960	T10957	punct	",",E
R3314	T10961	T10957	punct	),E
R3315	T10962	T10940	punct	.,demonstrated
R3316	T10964	T10965	advmod	Likewise,failed
R3317	T10966	T10967	det	the,glands
R3318	T10967	T10965	nsubj	glands,failed
R3319	T10968	T10967	amod	parathyroid,glands
R332	T2769	T2768	amod	phenotypic,transformation
R3320	T10969	T10970	aux	to,migrate
R3321	T10970	T10965	xcomp	migrate,failed
R3322	T10971	T10970	advmod	normally,migrate
R3323	T10972	T10970	prep	in,migrate
R3324	T10973	T10974	compound	Alk5,Cre
R3325	T10974	T10978	compound	Cre,mutants
R3326	T10975	T10974	punct	/,Cre
R3327	T10976	T10974	compound	Wnt1,Cre
R3328	T10977	T10974	punct	-,Cre
R3329	T10978	T10972	pobj	mutants,in
R333	T2854	T2850	prep	from,separates
R3330	T10979	T10965	punct	.,failed
R3331	T10981	T10982	prep	During,migrate
R3332	T10983	T10984	amod	normal,development
R3333	T10984	T10981	pobj	development,During
R3334	T10985	T10982	punct	", ",migrate
R3335	T10986	T10987	det	the,parathyroids
R3336	T10987	T10982	nsubj	parathyroids,migrate
R3337	T10988	T10982	advmod	first,migrate
R3338	T10989	T10982	prep	in,migrate
R3339	T10990	T10989	pobj	association,in
R334	T2770	T2766	prep	from,undergo
R3340	T10991	T10990	prep	with,association
R3341	T10992	T10993	det	the,primordia
R3342	T10993	T10991	pobj	primordia,with
R3343	T10994	T10993	amod	thymic,primordia
R3344	T10995	T10982	punct	", ",migrate
R3345	T10996	T10997	mark	until,reach
R3346	T10997	T10982	advcl	reach,migrate
R3347	T10998	T10997	nsubj	they,reach
R3348	T10999	T11000	det	the,thyroids
R3349	T11000	T10997	dobj	thyroids,reach
R335	T2771	T2772	det	an,type
R3350	T11001	T11000	prep	in,thyroids
R3351	T11002	T11003	det	the,region
R3352	T11003	T11001	pobj	region,in
R3353	T11004	T11003	compound	neck,region
R3354	T11005	T11006	mark	as,seen
R3355	T11006	T10997	advcl	seen,reach
R3356	T11007	T11006	prep	in,seen
R3357	T11008	T11009	compound	Fig,8C
R3358	T11009	T11007	pobj	8C,in
R3359	T11010	T10982	punct	.,migrate
R336	T2855	T2856	det	the,aorta
R3360	T11012	T11013	prep	In,remained
R3361	T11014	T11015	compound	Alk5,Cre
R3362	T11015	T11019	compound	Cre,mutants
R3363	T11016	T11015	punct	/,Cre
R3364	T11017	T11015	compound	Wnt1,Cre
R3365	T11018	T11015	punct	-,Cre
R3366	T11019	T11012	pobj	mutants,In
R3367	T11020	T11013	punct	", ",remained
R3368	T11021	T11022	det	the,parathyroids
R3369	T11022	T11013	nsubj	parathyroids,remained
R337	T2772	T2770	pobj	type,from
R3370	T11023	T11013	acomp	associated,remained
R3371	T11024	T11023	prep	with,associated
R3372	T11025	T11026	det	the,primordia
R3373	T11026	T11024	pobj	primordia,with
R3374	T11027	T11026	amod	thymic,primordia
R3375	T11028	T11013	punct	", ",remained
R3376	T11029	T11013	cc	and,remained
R3377	T11030	T11031	prep	despite,was
R3378	T11031	T11013	conj	was,remained
R3379	T11032	T11033	det	this,location
R338	T2773	T2772	amod	epithelial,type
R3380	T11033	T11030	pobj	location,despite
R3381	T11034	T11033	amod	abnormal,location
R3382	T11035	T11033	amod	rostral,location
R3383	T11036	T11031	punct	", ",was
R3384	T11037	T11031	nsubj	expression,was
R3385	T11038	T11037	prep	of,expression
R3386	T11039	T11040	compound	parathyroid,hormone
R3387	T11040	T11038	pobj	hormone,of
R3388	T11041	T11031	acomp	indistinguishable,was
R3389	T11042	T11031	prep	between,was
R339	T2774	T2773	prep	to,epithelial
R3390	T11043	T11044	compound	Alk5,mutants
R3391	T11044	T11042	pobj	mutants,between
R3392	T11045	T11044	cc	and,mutants
R3393	T11046	T11044	conj	controls,mutants
R3394	T11047	T11031	prep	at,was
R3395	T11048	T11047	pobj	E14,at
R3396	T11049	T11050	punct	(,F
R3397	T11050	T11031	parataxis	F,was
R3398	T11051	T11050	nmod	Fig.,F
R3399	T11052	T11050	nmod	8C,F
R34	T538	T539	amod	Congenital,diseases
R340	T2856	T2854	pobj	aorta,from
R3400	T11053	T11050	punct	",",F
R3401	T11054	T11050	punct	),F
R3402	T11055	T11013	punct	.,remained
R3403	T11057	T11058	aux	To,conclude
R3404	T11058	T11059	advcl	conclude,were
R3405	T11060	T11059	punct	", ",were
R3406	T11061	T11062	det	the,phenotypes
R3407	T11062	T11059	nsubj	phenotypes,were
R3408	T11063	T11062	amod	observed,phenotypes
R3409	T11064	T11065	amod	pharyngeal,organ
R341	T2775	T2774	amod	mesenchymal,to
R3410	T11065	T11062	compound	organ,phenotypes
R3411	T11066	T11059	advmod	also,were
R3412	T11067	T11059	prep	in,were
R3413	T11068	T11069	compound	striking,contrast
R3414	T11069	T11067	pobj	contrast,in
R3415	T11070	T11069	prep	to,contrast
R3416	T11071	T11070	pobj	those,to
R3417	T11072	T11071	acl	seen,those
R3418	T11073	T11072	prep	in,seen
R3419	T11074	T11075	compound	Tgfbr2,Cre
R342	T2776	T2772	compound	cell,type
R3420	T11075	T11079	compound	Cre,mutants
R3421	T11076	T11075	punct	/,Cre
R3422	T11077	T11075	compound	Wnt1,Cre
R3423	T11078	T11075	punct	-,Cre
R3424	T11079	T11073	pobj	mutants,in
R3425	T11080	T11081	punct	[,9
R3426	T11081	T11059	parataxis	9,were
R3427	T11082	T11081	nummod	8,9
R3428	T11083	T11081	punct	",",9
R3429	T11084	T11081	punct	],9
R343	T2857	T2858	punct	[,4
R3430	T11085	T11059	punct	.,were
R344	T2777	T2766	punct	", ",undergo
R345	T2778	T2766	cc	and,undergo
R3452	T13939	T13940	prep	During,proven
R3453	T13941	T13942	det	the,years
R3454	T13942	T13939	pobj	years,During
R3455	T13943	T13942	amod	last,years
R3456	T13944	T13942	amod	few,years
R3457	T13945	T13946	det	the,line
R3458	T13946	T13940	nsubj	line,proven
R3459	T13947	T13948	compound	Wnt1,Cre
R346	T2779	T2766	conj	migrate,undergo
R3460	T13948	T13950	npadvmod	Cre,transgenic
R3461	T13949	T13948	punct	-,Cre
R3462	T13950	T13946	amod	transgenic,line
R3463	T13951	T13946	compound	driver,line
R3464	T13952	T13940	aux	has,proven
R3465	T13953	T13954	aux	to,be
R3466	T13954	T13940	xcomp	be,proven
R3467	T13955	T13956	det	a,tool
R3468	T13956	T13954	attr	tool,be
R3469	T13957	T13956	amod	powerful,tool
R347	T2858	T2850	parataxis	4,separates
R3470	T13958	T13956	prep	for,tool
R3471	T13959	T13960	npadvmod	tissue,specific
R3472	T13960	T13962	amod	specific,deletion
R3473	T13961	T13960	punct	-,specific
R3474	T13962	T13958	pobj	deletion,for
R3475	T13963	T13962	compound	gene,deletion
R3476	T13964	T13962	prep	in,deletion
R3477	T13965	T13964	pobj	NCCs,in
R3478	T13966	T13967	punct	[,28
R3479	T13967	T13954	parataxis	28,be
R348	T2780	T2781	advmod	latero,ventrally
R3480	T13968	T13967	nummod	12,28
R3481	T13969	T13967	punct	",",28
R3482	T13970	T13967	nummod	13,28
R3483	T13971	T13967	punct	",",28
R3484	T13972	T13967	nummod	27,28
R3485	T13973	T13967	punct	",",28
R3486	T13974	T13967	punct	],28
R3487	T13975	T13940	punct	.,proven
R3488	T13977	T13978	advcl	Using,shown
R3489	T13979	T13980	det	this,approach
R349	T2781	T2779	advmod	ventrally,migrate
R3490	T13980	T13977	dobj	approach,Using
R3491	T13981	T13978	punct	", ",shown
R3492	T13982	T13983	amod	several,studies
R3493	T13983	T13978	nsubj	studies,shown
R3494	T13984	T13978	aux	have,shown
R3495	T13985	T13978	advmod	independently,shown
R3496	T13986	T13987	mark	that,leads
R3497	T13987	T13978	ccomp	leads,shown
R3498	T13988	T13989	det	the,deletion
R3499	T13989	T13987	nsubj	deletion,leads
R35	T539	T541	nsubj	diseases,are
R350	T2782	T2781	punct	-,ventrally
R3500	T13990	T13991	npadvmod	NC,specific
R3501	T13991	T13989	amod	specific,deletion
R3502	T13992	T13991	punct	-,specific
R3503	T13993	T13989	prep	of,deletion
R3504	T13994	T13995	det	the,gene
R3505	T13995	T13993	pobj	gene,of
R3506	T13996	T13995	compound	Tgfbr2,gene
R3507	T13997	T13987	prep	to,leads
R3508	T13998	T13999	det	a,set
R3509	T13999	T13997	pobj	set,to
R351	T2783	T2779	prep	into,migrate
R3510	T14000	T13999	amod	distinct,set
R3511	T14001	T13999	prep	of,set
R3512	T14002	T14003	amod	calvarial,defects
R3513	T14003	T14001	pobj	defects,of
R3514	T14004	T14002	punct	", ",calvarial
R3515	T14005	T14002	conj	facial,calvarial
R3516	T14006	T14005	cc	and,facial
R3517	T14007	T14005	conj	cardiac,facial
R3518	T14008	T14009	punct	[,29
R3519	T14009	T13978	parataxis	29,shown
R352	T2859	T2858	punct	],4
R3520	T14010	T14009	nummod	8,29
R3521	T14011	T14009	punct	",",29
R3522	T14012	T14009	nummod	9,29
R3523	T14013	T14009	punct	",",29
R3524	T14014	T14009	nummod	20,29
R3525	T14015	T14009	punct	",",29
R3526	T14016	T14009	punct	],29
R3527	T14017	T13978	punct	.,shown
R3528	T14019	T14020	advmod	Interestingly,appear
R3529	T14021	T14020	punct	", ",appear
R353	T2784	T2785	det	the,arteries
R3530	T14022	T14023	det	these,defects
R3531	T14023	T14020	nsubj	defects,appear
R3532	T14024	T14025	advmod	quite,different
R3533	T14025	T14020	oprd	different,appear
R3534	T14026	T14027	preconj	both,in
R3535	T14027	T14020	prep	in,appear
R3536	T14028	T14029	det	the,regions
R3537	T14029	T14027	pobj	regions,in
R3538	T14030	T14029	amod	craniofacial,regions
R3539	T14031	T14030	cc	and,craniofacial
R354	T2860	T2827	punct	.,continues
R3540	T14032	T14030	conj	pharyngeal,craniofacial
R3541	T14033	T14029	punct	", ",regions
R3542	T14034	T14029	prep	including,regions
R3543	T14035	T14036	det	the,heart
R3544	T14036	T14034	pobj	heart,including
R3545	T14037	T14020	punct	", ",appear
R3546	T14038	T14039	advmod	when,compared
R3547	T14039	T14020	advcl	compared,appear
R3548	T14040	T14039	prep	to,compared
R3549	T14041	T14042	det	the,mutants
R355	T2785	T2783	pobj	arteries,into
R3550	T14042	T14040	pobj	mutants,to
R3551	T14043	T14042	amod	corresponding,mutants
R3552	T14044	T14042	prep	of,mutants
R3553	T14045	T14044	pobj	Alk5,of
R3554	T14046	T14042	punct	", ",mutants
R3555	T14047	T14048	dep	which,encodes
R3556	T14048	T14042	relcl	encodes,mutants
R3557	T14049	T14050	det	the,receptor
R3558	T14050	T14048	dobj	receptor,encodes
R3559	T14051	T14052	nmod	TGF,β
R356	T2862	T2863	det	An,role
R3560	T14052	T14050	nmod	β,receptor
R3561	T14053	T14052	punct	-,β
R3562	T14054	T14050	nmod	type,receptor
R3563	T14055	T14054	nummod	I,type
R3564	T14056	T14050	punct	", ",receptor
R3565	T14057	T14058	det	a,partner
R3566	T14058	T14050	appos	partner,receptor
R3567	T14059	T14058	amod	prototypical,partner
R3568	T14060	T14058	compound	binding,partner
R3569	T14061	T14058	prep	of,partner
R357	T2786	T2785	amod	3rd,arteries
R3570	T14062	T14063	compound	TGF,βRII
R3571	T14063	T14061	pobj	βRII,of
R3572	T14064	T14063	punct	-,βRII
R3573	T14065	T14066	punct	[,19
R3574	T14066	T14039	parataxis	19,compared
R3575	T14067	T14066	punct	],19
R3576	T14068	T14066	cc	and,19
R3577	T14069	T14070	det	the,study
R3578	T14070	T14066	conj	study,19
R3579	T14071	T14070	amod	present,study
R358	T2787	T2786	punct	", ",3rd
R3580	T14072	T14066	punct	),19
R3581	T14073	T14020	punct	.,appear
R3582	T14075	T14076	mark	While,display
R3583	T14076	T14092	advcl	display,demonstrate
R3584	T14077	T14078	compound	Tgfbr2,Cre
R3585	T14078	T14082	compound	Cre,mutants
R3586	T14079	T14078	punct	/,Cre
R3587	T14080	T14078	compound	Wnt1,Cre
R3588	T14081	T14078	punct	-,Cre
R3589	T14082	T14076	nsubj	mutants,display
R359	T2788	T2786	conj	4th,3rd
R3590	T14083	T14084	advmod	as,as
R3591	T14084	T14082	cc	as,mutants
R3592	T14085	T14084	advmod	well,as
R3593	T14086	T14082	conj	mice,mutants
R3594	T14087	T14086	amod	deficient,mice
R3595	T14088	T14087	prep	in,deficient
R3596	T14089	T14090	compound	Tgf,β2
R3597	T14090	T14088	pobj	β2,in
R3598	T14091	T14090	punct	-,β2
R3599	T14093	T14094	det	the,A4
R36	T540	T539	amod	cardiovascular,diseases
R360	T2863	T2865	nsubjpass	role,demonstrated
R3600	T14094	T14076	dobj	A4,display
R3601	T14095	T14094	compound	PTA,A4
R3602	T14096	T14094	compound	type,A4
R3603	T14097	T14094	punct	(,A4
R3604	T14098	T14099	compound	truncus,arteriosus
R3605	T14099	T14094	appos	arteriosus,A4
R3606	T14100	T14099	prep	with,arteriosus
R3607	T14101	T14102	amod	interrupted,arch
R3608	T14102	T14100	pobj	arch,with
R3609	T14103	T14102	amod	aortic,arch
R361	T2789	T2788	cc	and,4th
R3610	T14104	T14105	punct	[,30
R3611	T14105	T14076	parataxis	30,display
R3612	T14106	T14105	punct	],30
R3613	T14107	T14092	punct	),demonstrate
R3614	T14108	T14092	punct	", ",demonstrate
R3615	T14109	T14110	compound	Alk5,Cre
R3616	T14110	T14114	compound	Cre,mutants
R3617	T14111	T14110	punct	/,Cre
R3618	T14112	T14110	compound	Wnt1,Cre
R3619	T14113	T14110	punct	-,Cre
R362	T2790	T2788	conj	6th,4th
R3620	T14114	T14092	nsubj	mutants,demonstrate
R3621	T14115	T14114	acl	reported,mutants
R3622	T14116	T14115	advmod	here,reported
R3623	T14117	T14118	amod	earlier,defects
R3624	T14118	T14092	dobj	defects,demonstrate
R3625	T14119	T14118	compound	patterning,defects
R3626	T14120	T14118	prep	of,defects
R3627	T14121	T14122	det	the,PAAs
R3628	T14122	T14120	pobj	PAAs,of
R3629	T14123	T14118	punct	", ",defects
R363	T2791	T2785	amod	pharyngeal,arteries
R3630	T14124	T14125	dep	which,is
R3631	T14125	T14118	relcl	is,defects
R3632	T14126	T14127	advmod	particularly,obvious
R3633	T14127	T14125	acomp	obvious,is
R3634	T14128	T14125	prep	in,is
R3635	T14129	T14130	det	the,pair
R3636	T14130	T14128	pobj	pair,in
R3637	T14131	T14130	amod	3rd,pair
R3638	T14132	T14130	prep	of,pair
R3639	T14133	T14134	det	the,PAAs
R364	T2792	T2785	compound	arch,arteries
R3640	T14134	T14132	pobj	PAAs,of
R3641	T14135	T14092	punct	.,demonstrate
R3642	T14137	T14138	advmod	Moreover,display
R3643	T14139	T14138	punct	", ",display
R3644	T14140	T14141	det	the,mutants
R3645	T14141	T14138	nsubj	mutants,display
R3646	T14142	T14143	compound	Alk5,Cre
R3647	T14143	T14141	compound	Cre,mutants
R3648	T14144	T14143	punct	/,Cre
R3649	T14145	T14143	compound	Wnt1,Cre
R365	T2793	T2785	punct	(,arteries
R3650	T14146	T14143	punct	-,Cre
R3651	T14147	T14148	det	an,patterning
R3652	T14148	T14138	dobj	patterning,display
R3653	T14149	T14148	amod	abnormal,patterning
R3654	T14150	T14148	prep	of,patterning
R3655	T14151	T14152	det	the,sac
R3656	T14152	T14150	pobj	sac,of
R3657	T14153	T14152	amod	aortic,sac
R3658	T14154	T14148	cc	and,patterning
R3659	T14155	T14156	amod	defective,septation
R366	T2794	T2785	appos	PAAs,arteries
R3660	T14156	T14148	conj	septation,patterning
R3661	T14157	T14156	compound	AP,septation
R3662	T14158	T14156	acl	leading,septation
R3663	T14159	T14158	prep	to,leading
R3664	T14160	T14159	pobj	PTA,to
R3665	T14161	T14148	punct	", ",patterning
R3666	T14162	T14148	amod	reminiscent,patterning
R3667	T14163	T14162	prep	of,reminiscent
R3668	T14164	T14165	compound	type,A2
R3669	T14165	T14163	pobj	A2,of
R367	T2795	T2785	punct	),arteries
R3670	T14166	T14165	punct	(,A2
R3671	T14167	T14168	punct	=,artriosus
R3672	T14168	T14165	acl	artriosus,A2
R3673	T14169	T14168	compound	truncus,artriosus
R3674	T14170	T14168	prep	with,artriosus
R3675	T14171	T14172	det	no,segment
R3676	T14172	T14170	pobj	segment,with
R3677	T14173	T14172	amod	main,segment
R3678	T14174	T14172	amod	pulmonary,segment
R3679	T14175	T14172	compound	artery,segment
R368	T2864	T2863	amod	indispensable,role
R3680	T14176	T14172	amod	present,segment
R3681	T14177	T14178	punct	[,30
R3682	T14178	T14148	parataxis	30,patterning
R3683	T14179	T14178	punct	],30
R3684	T14180	T14138	punct	),display
R3685	T14181	T14138	punct	.,display
R3686	T14183	T14184	advmod	However,demonstrate
R3687	T14185	T14184	punct	", ",demonstrate
R3688	T14186	T14187	poss	our,results
R3689	T14187	T14184	nsubj	results,demonstrate
R369	T2796	T2785	punct	", ",arteries
R3690	T14188	T14184	advmod	also,demonstrate
R3691	T14189	T14190	mark	that,leads
R3692	T14190	T14184	ccomp	leads,demonstrate
R3693	T14191	T14192	amod	significant,hypoplasia
R3694	T14192	T14190	nsubj	hypoplasia,leads
R3695	T14193	T14192	prep	of,hypoplasia
R3696	T14194	T14195	det	the,sac
R3697	T14195	T14193	pobj	sac,of
R3698	T14196	T14195	amod	aortic,sac
R3699	T14197	T14190	prep	to,leads
R37	T542	T543	det	the,form
R370	T2797	T2798	advmod	where,contribute
R3700	T14198	T14199	det	a,shortening
R3701	T14199	T14197	pobj	shortening,to
R3702	T14200	T14199	amod	severe,shortening
R3703	T14201	T14199	prep	of,shortening
R3704	T14202	T14203	det	the,arch
R3705	T14203	T14201	pobj	arch,of
R3706	T14204	T14203	amod	ascending,arch
R3707	T14205	T14203	amod	truncal,arch
R3708	T14206	T14199	punct	", ",shortening
R3709	T14207	T14208	dep	which,masks
R371	T2798	T2785	relcl	contribute,arteries
R3710	T14208	T14199	relcl	masks,shortening
R3711	T14209	T14210	amod	possible,defects
R3712	T14210	T14208	dobj	defects,masks
R3713	T14211	T14210	prep	in,defects
R3714	T14212	T14211	pobj	derivatives,in
R3715	T14213	T14212	prep	of,derivatives
R3716	T14214	T14215	det	the,PAAs
R3717	T14215	T14213	pobj	PAAs,of
R3718	T14216	T14215	amod	4th,PAAs
R3719	T14217	T14210	punct	", ",defects
R372	T2866	T2863	prep	of,role
R3720	T14218	T14219	advmod	i.e.,interruption
R3721	T14219	T14210	appos	interruption,defects
R3722	T14220	T14219	punct	", ",interruption
R3723	T14221	T14219	prep	of,interruption
R3724	T14222	T14223	det	the,arch
R3725	T14223	T14221	pobj	arch,of
R3726	T14224	T14223	amod	aortic,arch
R3727	T14225	T14184	punct	.,demonstrate
R3728	T14227	T14228	det	These,differences
R3729	T14228	T14230	nsubj	differences,are
R373	T2799	T2798	nsubj	they,contribute
R3730	T14229	T14228	amod	observed,differences
R3731	T14231	T14232	advmod	likely,due
R3732	T14232	T14230	prep	due,are
R3733	T14233	T14232	pcomp	to,due
R3734	T14234	T14235	amod	substantial,apoptosis
R3735	T14235	T14232	pobj	apoptosis,due
R3736	T14236	T14235	prep	of,apoptosis
R3737	T14237	T14238	npadvmod	Alk5,deficient
R3738	T14238	T14240	amod	deficient,cells
R3739	T14239	T14238	punct	-,deficient
R374	T2800	T2798	prep	to,contribute
R3740	T14240	T14236	pobj	cells,of
R3741	T14241	T14240	amod	post-migratory,cells
R3742	T14242	T14243	amod	neural,crest
R3743	T14243	T14240	compound	crest,cells
R3744	T14244	T14235	punct	", ",apoptosis
R3745	T14245	T14246	dep	which,is
R3746	T14246	T14235	relcl	is,apoptosis
R3747	T14247	T14248	advmod	clearly,detectable
R3748	T14248	T14246	acomp	detectable,is
R3749	T14249	T14246	prep	at,is
R375	T2801	T2802	det	the,formation
R3750	T14250	T14249	pobj	E10.5,at
R3751	T14251	T14246	punct	", ",is
R3752	T14252	T14253	mark	whereas,reported
R3753	T14253	T14246	advcl	reported,is
R3754	T14254	T14255	amod	similar,death
R3755	T14255	T14253	nsubjpass	death,reported
R3756	T14256	T14255	amod	intense,death
R3757	T14257	T14255	compound	cell,death
R3758	T14258	T14253	aux	has,reported
R3759	T14259	T14253	neg	not,reported
R376	T2867	T2866	pobj	CNCCs,of
R3760	T14260	T14253	auxpass	been,reported
R3761	T14261	T14253	prep	in,reported
R3762	T14262	T14263	compound	Tgfbr2,Cre
R3763	T14263	T14267	compound	Cre,mutants
R3764	T14264	T14263	punct	/,Cre
R3765	T14265	T14263	compound	Wnt1,Cre
R3766	T14266	T14263	punct	-,Cre
R3767	T14267	T14261	pobj	mutants,in
R3768	T14268	T14269	punct	[,9
R3769	T14269	T14230	parataxis	9,are
R377	T2802	T2800	pobj	formation,to
R3770	T14270	T14269	punct	],9
R3771	T14271	T14230	punct	.,are
R3772	T14273	T14274	poss	Our,results
R3773	T14274	T14276	nsubj	results,suggest
R3774	T14275	T14274	amod	present,results
R3775	T14277	T14278	mark	that,mediates
R3776	T14278	T14276	ccomp	mediates,suggest
R3777	T14279	T14280	mark	while,mediated
R3778	T14280	T14278	advcl	mediated,mediates
R3779	T14281	T14282	compound	TGF,β
R378	T2803	T2802	prep	of,formation
R3780	T14282	T14284	compound	β,signaling
R3781	T14283	T14282	punct	-,β
R3782	T14284	T14280	nsubjpass	signaling,mediated
R3783	T14285	T14284	prep	in,signaling
R3784	T14286	T14287	amod	cardiac,NCCs
R3785	T14287	T14285	pobj	NCCs,in
R3786	T14288	T14280	auxpass	is,mediated
R3787	T14289	T14280	advmod	predominantly,mediated
R3788	T14290	T14280	prep	via,mediated
R3789	T14291	T14292	det	the,complex
R379	T2868	T2863	prep	in,role
R3790	T14292	T14290	pobj	complex,via
R3791	T14293	T14294	compound	ALK5,βRII
R3792	T14294	T14298	compound	βRII,receptor
R3793	T14295	T14294	punct	/,βRII
R3794	T14296	T14294	compound	TGF,βRII
R3795	T14297	T14294	punct	-,βRII
R3796	T14298	T14292	compound	receptor,complex
R3797	T14299	T14278	punct	", ",mediates
R3798	T14300	T14278	nsubj	ALK5,mediates
R3799	T14301	T14278	advmod	also,mediates
R38	T543	T541	attr	form,are
R380	T2804	T2805	det	the,layer
R3800	T14302	T14278	dobj	signaling,mediates
R3801	T14303	T14302	prep	of,signaling
R3802	T14304	T14305	amod	other,ligands
R3803	T14305	T14303	pobj	ligands,of
R3804	T14306	T14305	amod	related,ligands
R3805	T14307	T14305	punct	", ",ligands
R3806	T14308	T14309	dep	which,required
R3807	T14309	T14305	relcl	required,ligands
R3808	T14310	T14309	auxpass	are,required
R3809	T14311	T14312	preconj	either,directly
R381	T2805	T2803	pobj	layer,of
R3810	T14312	T14309	advmod	directly,required
R3811	T14361	T14362	advmod	Furthermore,induce
R3812	T14313	T14312	cc	or,directly
R3813	T14363	T14362	punct	", ",induce
R3814	T14314	T14312	conj	indirectly,directly
R3815	T14364	T14365	det	a,subset
R3816	T14365	T14362	nsubj	subset,induce
R3817	T14315	T14309	prep	for,required
R3818	T14366	T14365	prep	of,subset
R3819	T14367	T14368	compound	TGF,β
R382	T2806	T2807	amod	smooth,cell
R3820	T14368	T14370	npadvmod	β,related
R3821	T14316	T14317	amod	appropriate,survival
R3822	T14369	T14368	punct	-,β
R3823	T14370	T14372	amod	related,factors
R3824	T14317	T14315	pobj	survival,for
R3825	T14371	T14370	punct	-,related
R3826	T14372	T14366	pobj	factors,of
R3827	T14373	T14372	nmod	growth,factors
R3828	T14318	T14317	compound	NCC,survival
R3829	T14374	T14373	cc	and,growth
R383	T2869	T2870	det	the,development
R3830	T14375	T14373	conj	differentiation,growth
R3831	T14376	T14372	punct	(,factors
R3832	T14319	T14276	punct	.,suggest
R3833	T14377	T14372	appos	GDFs,factors
R3834	T14378	T14365	punct	),subset
R3835	T14379	T14365	punct	", ",subset
R3836	T14321	T14322	prep	In,shown
R3837	T14380	T14381	advmod	e.g.,GDF8
R3838	T14381	T14365	appos	GDF8,subset
R3839	T14382	T14381	punct	", ",GDF8
R384	T2807	T2805	compound	cell,layer
R3840	T14383	T14381	punct	", ",GDF8
R3841	T14323	T14321	pobj	fact,In
R3842	T14384	T14381	conj	GDF9,GDF8
R3843	T14385	T14384	punct	", ",GDF9
R3844	T14386	T14384	conj	GDF11,GDF9
R3845	T14387	T14386	cc	and,GDF11
R3846	T14388	T14386	conj	GDF15,GDF11
R3847	T14389	T14362	aux	could,induce
R3848	T14324	T14322	punct	", ",shown
R3849	T14390	T14391	det	these,events
R385	T2808	T2807	compound	muscle,cell
R3850	T14391	T14362	dobj	events,induce
R3851	T14325	T14322	nsubjpass	it,shown
R3852	T14392	T14393	punct	[,33
R3853	T14393	T14362	parataxis	33,induce
R3854	T14394	T14393	nummod	17,33
R3855	T14395	T14393	punct	",",33
R3856	T14396	T14393	nummod	18,33
R3857	T14326	T14322	aux	has,shown
R3858	T14397	T14393	punct	",",33
R3859	T14398	T14393	nummod	32,33
R386	T2809	T2805	prep	of,layer
R3860	T14399	T14393	punct	",",33
R3861	T14327	T14322	auxpass	been,shown
R3862	T14400	T14393	punct	],33
R3863	T14401	T14362	punct	.,induce
R3864	T14328	T14329	mark	that,form
R3865	T14329	T14322	ccomp	form,shown
R3866	T14403	T14404	mark	Although,display
R3867	T14330	T14329	punct	", ",form
R3868	T14404	T14429	advcl	display,exclude
R3869	T14405	T14406	amod	relevant,8
R387	T2870	T2868	pobj	development,in
R3870	T14331	T14329	prep	besides,form
R3871	T14406	T14408	nsubjpass	8,expressed
R3872	T14407	T14406	nmod	Gdfs,8
R3873	T14408	T14404	ccomp	expressed,display
R3874	T14332	T14333	compound	TGF,βRII
R3875	T14409	T14406	punct	", ",8
R3876	T14410	T14411	nummod	9,11
R3877	T14333	T14331	pobj	βRII,besides
R3878	T14411	T14406	conj	11,8
R3879	T14412	T14411	cc	and,11
R388	T2810	T2811	amod	endothelial,structures
R3880	T14413	T14411	conj	15,11
R3881	T14334	T14333	punct	-,βRII
R3882	T14414	T14408	auxpass	are,expressed
R3883	T14415	T14408	neg	not,expressed
R3884	T14335	T14329	punct	", ",form
R3885	T14416	T14408	prep	in,expressed
R3886	T14417	T14418	det	the,heart
R3887	T14418	T14416	pobj	heart,in
R3888	T14336	T14329	nsubj	ALK5,form
R3889	T14419	T14418	amod	developing,heart
R389	T2811	T2809	pobj	structures,of
R3890	T14420	T14404	punct	", ",display
R3891	T14421	T14404	cc	nor,display
R3892	T14337	T14329	aux	can,form
R3893	T14422	T14404	aux	do,display
R3894	T14423	T14424	det	the,mice
R3895	T14424	T14404	nsubj	mice,display
R3896	T14425	T14424	amod	deficient,mice
R3897	T14426	T14425	prep	in,deficient
R3898	T14338	T14329	advmod	also,form
R3899	T14427	T14428	det	these,Gdfs
R39	T544	T545	advmod	most,common
R390	T2871	T2870	prep	of,development
R3900	T14428	T14426	pobj	Gdfs,in
R3901	T14339	T14340	det	a,complex
R3902	T14430	T14431	amod	developmental,defects
R3903	T14431	T14404	dobj	defects,display
R3904	T14340	T14329	dobj	complex,form
R3905	T14432	T14431	amod	cardiac,defects
R3906	T14341	T14340	prep	with,complex
R3907	T14433	T14429	punct	", ",exclude
R3908	T14434	T14429	nsubj	we,exclude
R3909	T14435	T14429	aux	can,exclude
R391	T2812	T2811	acl	derived,structures
R3910	T14436	T14429	neg	not,exclude
R3911	T14342	T14343	det	the,receptor
R3912	T14437	T14438	det	the,possibility
R3913	T14438	T14429	dobj	possibility,exclude
R3914	T14439	T14440	mark	that,contribute
R3915	T14343	T14341	pobj	receptor,with
R3916	T14440	T14438	acl	contribute,possibility
R3917	T14441	T14442	amod	circulating,GDFs
R3918	T14344	T14343	compound	Activin,receptor
R3919	T14442	T14440	nsubj	GDFs,contribute
R392	T2813	T2812	prep	from,derived
R3920	T14443	T14442	punct	", ",GDFs
R3921	T14444	T14445	advmod	perhaps,in
R3922	T14345	T14346	compound	type,IIB
R3923	T14445	T14442	prep	in,GDFs
R3924	T14446	T14445	pobj	concert,in
R3925	T14447	T14446	prep	with,concert
R3926	T14346	T14343	compound	IIB,receptor
R3927	T14448	T14449	compound	TGF,βs
R3928	T14449	T14447	pobj	βs,with
R3929	T14450	T14449	punct	-,βs
R393	T2814	T2815	det	the,arteries
R3930	T14347	T14348	aux	to,activate
R3931	T14451	T14440	aux	may,contribute
R3932	T14452	T14440	prep	to,contribute
R3933	T14453	T14454	compound	NCC,survival
R3934	T14348	T14329	advcl	activate,form
R3935	T14454	T14452	pobj	survival,to
R3936	T14455	T14440	prep	during,contribute
R3937	T14456	T14457	amod	cardiac,morphogenesis
R3938	T14349	T14350	amod	downstream,Smads
R3939	T14457	T14455	pobj	morphogenesis,during
R394	T2872	T2873	det	the,tract
R3940	T14458	T14456	cc	and,cardiac
R3941	T14459	T14456	conj	pharyngeal,cardiac
R3942	T14350	T14348	dobj	Smads,activate
R3943	T14460	T14429	punct	.,exclude
R3944	T14351	T14350	nummod	2,Smads
R3945	T14462	T14463	nsubj	We,studied
R3946	T14352	T14350	punct	/,Smads
R3947	T14464	T14463	advmod	specifically,studied
R3948	T14465	T14463	dobj	apoptosis,studied
R3949	T14353	T14350	nummod	3,Smads
R395	T2815	T2813	pobj	arteries,from
R3950	T14354	T14355	punct	[,31
R3951	T14466	T14465	prep	at,apoptosis
R3952	T14467	T14468	det	the,level
R3953	T14355	T14329	parataxis	31,form
R3954	T14468	T14466	pobj	level,at
R3955	T14469	T14468	prep	of,level
R3956	T14470	T14471	det	the,sac
R3957	T14356	T14355	nummod	18,31
R3958	T14471	T14469	pobj	sac,of
R3959	T14472	T14471	amod	aortic,sac
R396	T2816	T2817	amod	aortic,arch
R3960	T14473	T14471	punct	", ",sac
R3961	T14357	T14355	punct	",",31
R3962	T14474	T14475	advmod	where,forms
R3963	T14358	T14355	punct	],31
R3964	T14475	T14471	relcl	forms,sac
R3965	T14476	T14477	det	the,septum
R3966	T14477	T14475	nsubj	septum,forms
R3967	T14478	T14477	compound	AP,septum
R3968	T14359	T14322	punct	.,shown
R3969	T14479	T14477	punct	-,septum
R397	T2817	T2815	compound	arch,arteries
R3970	T14480	T14475	prep	between,forms
R3971	T14481	T14482	det	the,origins
R3972	T14572	T14564	prep	between,forming
R3973	T14482	T14480	pobj	origins,between
R3974	T14483	T14482	prep	of,origins
R3975	T14484	T14485	amod	4th,PAAs
R3976	T14573	T14572	pobj	E10.5,between
R3977	T14485	T14483	pobj	PAAs,of
R3978	T14486	T14484	cc	and,4th
R3979	T14487	T14484	conj	6th,4th
R398	T2873	T2871	pobj	tract,of
R3980	T14574	T14573	cc	and,E10.5
R3981	T14488	T14463	punct	.,studied
R3982	T14490	T14491	advmod	Already,see
R3983	T14575	T14573	conj	E11.5,E10.5
R3984	T14491	T14497	ccomp	see,precedes
R3985	T14492	T14491	prep	at,see
R3986	T14576	T14542	punct	.,detect
R3987	T14493	T14492	pobj	E10.5,at
R3988	T14494	T14491	punct	", ",see
R3989	T14495	T14491	nsubj	we,see
R399	T2874	T2873	amod	cardiac,tract
R3990	T14578	T14579	det	These,findings
R3991	T14496	T14491	aux	could,see
R3992	T14498	T14499	amod	intense,apoptosis
R3993	T14579	T14580	nsubj	findings,suggest
R3994	T14499	T14491	dobj	apoptosis,see
R3995	T14500	T14499	prep	among,apoptosis
R3996	T14501	T14502	det	the,NCCs
R3997	T14581	T14582	mark	that,affected
R3998	T14502	T14500	pobj	NCCs,among
R3999	T14503	T14502	amod	postmigratory,NCCs
R40	T545	T543	amod	common,form
R400	T2818	T2819	punct	[,1
R4000	T14504	T14491	prep	in,see
R4001	T14505	T14506	det	the,mesenchyme
R4002	T14506	T14504	pobj	mesenchyme,in
R4003	T14507	T14506	acl	surrounding,mesenchyme
R4004	T14582	T14580	ccomp	affected,suggest
R4005	T14508	T14509	det	the,sac
R4006	T14509	T14507	dobj	sac,surrounding
R4007	T14510	T14509	amod	aortic,sac
R4008	T14511	T14491	prep	at,see
R4009	T14583	T14584	det	the,pool
R401	T2819	T2798	parataxis	1,contribute
R4010	T14512	T14513	det	the,site
R4011	T14513	T14511	pobj	site,at
R4012	T14514	T14515	advmod	where,forms
R4013	T14584	T14582	nsubjpass	pool,affected
R4014	T14515	T14513	relcl	forms,site
R4015	T14516	T14517	det	the,septum
R4016	T14517	T14515	nsubj	septum,forms
R4017	T14585	T14584	prep	of,pool
R4018	T14518	T14517	amod	prospective,septum
R4019	T14519	T14517	compound	AP,septum
R402	T2875	T2873	compound	outflow,tract
R4020	T14520	T14497	punct	", ",precedes
R4021	T14586	T14585	pobj	cells,of
R4022	T14521	T14497	advmod	i.e.,precedes
R4023	T14522	T14497	punct	", ",precedes
R4024	T14523	T14524	det	this,death
R4025	T14587	T14584	acl	forming,pool
R4026	T14524	T14497	nsubj	death,precedes
R4027	T14525	T14524	compound	cell,death
R4028	T14526	T14527	det	the,defect
R4029	T14588	T14589	det	the,septum
R403	T2820	T2821	punct	-,3
R4030	T14527	T14497	dobj	defect,precedes
R4031	T14528	T14527	nmod	AP,defect
R4032	T14529	T14527	amod	septal,defect
R4033	T14589	T14587	dobj	septum,forming
R4034	T14530	T14527	acl	seen,defect
R4035	T14531	T14530	prep	in,seen
R4036	T14590	T14589	compound	AP,septum
R4037	T14532	T14531	pobj	mutants,in
R4038	T14533	T14497	punct	.,precedes
R4039	T14591	T14582	auxpass	is,affected
R404	T2821	T2819	prep	3,1
R4040	T14535	T14536	mark	Although,appeared
R4041	T14536	T14542	advcl	appeared,detect
R4042	T14592	T14582	advmod	severely,affected
R4043	T14537	T14538	det	some,cells
R4044	T14538	T14536	nsubj	cells,appeared
R4045	T14539	T14540	npadvmod	NC,derived
R4046	T14593	T14582	agent	by,affected
R4047	T14540	T14538	amod	derived,cells
R4048	T14541	T14540	punct	-,derived
R4049	T14594	T14595	det	the,death
R405	T2822	T2819	punct	],1
R4050	T14543	T14544	aux	to,differentiating
R4051	T14544	T14536	xcomp	differentiating,appeared
R4052	T14545	T14544	aux	be,differentiating
R4053	T14546	T14544	prep	to,differentiating
R4054	T14547	T14548	amod	smooth,cells
R4055	T14595	T14593	pobj	death,by
R4056	T14548	T14546	pobj	cells,to
R4057	T14549	T14548	compound	muscle,cells
R4058	T14550	T14544	prep	in,differentiating
R4059	T14596	T14595	compound	cell,death
R406	T2876	T2865	auxpass	was,demonstrated
R4060	T14551	T14552	det	the,OFT
R4061	T14552	T14550	pobj	OFT,in
R4062	T14553	T14554	punct	(,Fig.
R4063	T14597	T14580	punct	.,suggest
R4064	T14554	T14544	parataxis	Fig.,differentiating
R4065	T14555	T14554	nummod	6,Fig.
R4066	T14556	T14554	punct	),Fig.
R4067	T14599	T14600	advmod	Moreover,is
R4068	T14557	T14542	punct	", ",detect
R4069	T14558	T14542	nsubj	we,detect
R407	T2823	T2757	punct	.,delaminate
R4070	T14559	T14542	aux	could,detect
R4071	T14560	T14542	neg	never,detect
R4072	T14601	T14600	punct	", ",is
R4073	T14561	T14562	det	the,septum
R4074	T14562	T14564	nsubj	septum,forming
R4075	T14563	T14562	compound	AP,septum
R4076	T14564	T14542	ccomp	forming,detect
R4077	T14602	T14600	nsubj	it,is
R4078	T14565	T14564	prep	in,forming
R4079	T14566	T14567	compound	Alk5,Cre
R408	T2825	T2826	det	A,subset
R4080	T14567	T14571	compound	Cre,mutants
R4081	T14603	T14600	acomp	likely,is
R4082	T14568	T14567	punct	/,Cre
R4083	T14569	T14567	compound	Wnt1,Cre
R4084	T14570	T14567	punct	-,Cre
R4085	T14604	T14605	mark	that,die
R4086	T14571	T14565	pobj	mutants,in
R4087	T14605	T14600	ccomp	die,is
R4088	T14606	T14607	det	these,cells
R4089	T14607	T14605	nsubj	cells,die
R409	T2877	T2865	advmod	originally,demonstrated
R4090	T14608	T14607	acl	forming,cells
R4091	T14678	T14679	punct	[,9
R4092	T14609	T14610	det	the,septum
R4093	T14679	T14652	parataxis	9,suggested
R4094	T14680	T14679	punct	],9
R4095	T14610	T14608	dobj	septum,forming
R4096	T14681	T14652	punct	", ",suggested
R4097	T14682	T14683	mark	although,disputed
R4098	T14611	T14610	compound	AP,septum
R4099	T14683	T14652	advcl	disputed,suggested
R41	T546	T543	prep	of,form
R410	T2826	T2827	nsubj	subset,continues
R4100	T14684	T14685	det	this,result
R4101	T14685	T14683	nsubjpass	result,disputed
R4102	T14612	T14610	punct	-,septum
R4103	T14686	T14683	auxpass	was,disputed
R4104	T14687	T14683	advmod	later,disputed
R4105	T14688	T14683	agent	by,disputed
R4106	T14689	T14690	det	another,study
R4107	T14690	T14688	pobj	study,by
R4108	T14613	T14605	dep	the,die
R4109	T14691	T14692	punct	[,8
R411	T2878	T2865	agent	by,demonstrated
R4110	T14692	T14683	parataxis	8,disputed
R4111	T14693	T14692	punct	],8
R4112	T14614	T14615	mark	before,differentiate
R4113	T14694	T14652	punct	.,suggested
R4114	T14615	T14605	advcl	differentiate,die
R4115	T14696	T14697	poss	Our,staining
R4116	T14697	T14699	nsubj	staining,demonstrated
R4117	T14698	T14697	amod	immunohistochemical,staining
R4118	T14616	T14615	nsubj	majority,differentiate
R4119	T14700	T14697	prep	of,staining
R412	T2828	T2826	prep	of,subset
R4120	T14617	T14616	prep	of,majority
R4121	T14701	T14700	pobj	αSMA,of
R4122	T14702	T14697	prep	in,staining
R4123	T14703	T14704	det	the,OFT
R4124	T14618	T14617	pobj	them,of
R4125	T14704	T14702	pobj	OFT,in
R4126	T14619	T14615	aux	can,differentiate
R4127	T14705	T14699	advmod	unequivocally,demonstrated
R4128	T14706	T14707	mark	that,required
R4129	T14707	T14699	ccomp	required,demonstrated
R413	T2829	T2828	pobj	CNCCs,of
R4130	T14620	T14615	prep	to,differentiate
R4131	T14708	T14707	nsubjpass	signaling,required
R4132	T14709	T14708	prep	via,signaling
R4133	T14710	T14709	pobj	ALK5,via
R4134	T14621	T14622	amod	smooth,cells
R4135	T14711	T14707	auxpass	is,required
R4136	T14712	T14707	neg	not,required
R4137	T14622	T14620	pobj	cells,to
R4138	T14713	T14707	prep	for,required
R4139	T14714	T14715	amod	smooth,muscle
R414	T2830	T2831	aux	to,migrate
R4140	T14715	T14716	compound	muscle,differentiation
R4141	T14623	T14622	compound	muscle,cells
R4142	T14716	T14713	pobj	differentiation,for
R4143	T14717	T14718	advmod	in,vivo
R4144	T14624	T14600	punct	.,is
R4145	T14718	T14707	advmod	vivo,required
R4146	T14719	T14699	punct	.,demonstrated
R4147	T14626	T14627	amod	Several,studies
R4148	T14721	T14722	advmod	Moreover,suggested
R4149	T14723	T14722	punct	", ",suggested
R415	T2831	T2827	xcomp	migrate,continues
R4150	T14627	T14630	nsubj	studies,suggested
R4151	T14724	T14722	nsubjpass	it,suggested
R4152	T14725	T14722	aux	has,suggested
R4153	T14726	T14722	auxpass	been,suggested
R4154	T14628	T14629	advmod	in,vitro
R4155	T14727	T14728	mark	that,leads
R4156	T14728	T14722	ccomp	leads,suggested
R4157	T14729	T14728	nsubj	deletion,leads
R4158	T14730	T14729	prep	of,deletion
R4159	T14731	T14730	pobj	Tgfbr2,of
R416	T2832	T2831	advmod	deeper,migrate
R4160	T14732	T14729	prep	in,deletion
R4161	T14629	T14627	amod	vitro,studies
R4162	T14733	T14732	pobj	NCCs,in
R4163	T14734	T14728	prep	to,leads
R4164	T14735	T14736	amod	other,features
R4165	T14631	T14630	aux	have,suggested
R4166	T14736	T14734	pobj	features,to
R4167	T14737	T14736	amod	phenotypic,features
R4168	T14738	T14736	amod	reminiscent,features
R4169	T14739	T14738	prep	of,reminiscent
R417	T2833	T2831	prep	into,migrate
R4170	T14632	T14633	det	an,role
R4171	T14740	T14739	pobj	those,of
R4172	T14741	T14740	acl	seen,those
R4173	T14742	T14741	prep	in,seen
R4174	T14633	T14630	dobj	role,suggested
R4175	T14743	T14744	det	the,syndrome
R4176	T14634	T14633	amod	indispensable,role
R4177	T14744	T14742	pobj	syndrome,in
R4178	T14635	T14633	prep	for,role
R4179	T14745	T14746	amod	velocardiofacial,DiGeorge
R418	T2834	T2835	det	the,sac
R4180	T14746	T14744	compound	DiGeorge,syndrome
R4181	T14747	T14746	punct	/,DiGeorge
R4182	T14636	T14637	compound	TGF,β
R4183	T14748	T14744	punct	(,syndrome
R4184	T14749	T14750	compound	VCF,DGS
R4185	T14637	T14635	pobj	β,for
R4186	T14750	T14744	appos	DGS,syndrome
R4187	T14751	T14750	punct	/,DGS
R4188	T14752	T14741	punct	),seen
R4189	T14638	T14637	punct	-,β
R419	T2879	T2880	amod	pioneering,studies
R4190	T14753	T14754	punct	[,9
R4191	T14754	T14741	parataxis	9,seen
R4192	T14639	T14637	punct	-,β
R4193	T14755	T14754	punct	],9
R4194	T14756	T14736	acl	caused,features
R4195	T14640	T14637	amod	signaling,β
R4196	T14757	T14756	agent	by,caused
R4197	T14758	T14759	det	a,deletion
R4198	T14759	T14757	pobj	deletion,by
R4199	T14641	T14633	prep	in,role
R42	T547	T548	compound	birth,defects
R420	T2835	T2833	pobj	sac,into
R4200	T14760	T14759	prep	of,deletion
R4201	T14761	T14762	det	the,region
R4202	T14642	T14641	pobj	differentiation,in
R4203	T14762	T14760	pobj	region,of
R4204	T14763	T14764	advmod	so,called
R4205	T14643	T14642	prep	of,differentiation
R4206	T14764	T14762	amod	called,region
R4207	T14765	T14762	nmod	DiGeorge,region
R4208	T14766	T14762	amod	critical,region
R4209	T14767	T14762	punct	(,region
R421	T2836	T2835	amod	aortic,sac
R4210	T14768	T14762	appos	DGCR,region
R4211	T14769	T14759	punct	),deletion
R4212	T14644	T14643	pobj	NCCs,of
R4213	T14770	T14759	prep	on,deletion
R4214	T14771	T14772	compound	chromosome,22q11
R4215	T14645	T14644	prep	into,NCCs
R4216	T14772	T14770	pobj	22q11,on
R4217	T14773	T14774	punct	[,35
R4218	T14774	T14722	parataxis	35,suggested
R4219	T14646	T14647	amod	smooth,muscle
R422	T2880	T2878	pobj	studies,by
R4220	T14775	T14774	nummod	34,35
R4221	T14776	T14774	punct	",",35
R4222	T14647	T14648	compound	muscle,cells
R4223	T14777	T14774	punct	],35
R4224	T14778	T14722	punct	.,suggested
R4225	T14648	T14645	pobj	cells,into
R4226	T14780	T14781	poss	Our,results
R4227	T14781	T14783	nsubj	results,suggest
R4228	T14649	T14630	punct	.,suggested
R4229	T14782	T14781	amod	present,results
R423	T2881	T2880	prep	of,studies
R4230	T14651	T14652	advmod	Moreover,suggested
R4231	T14653	T14652	punct	", ",suggested
R4232	T14784	T14785	mark	that,shows
R4233	T14654	T14655	det	a,study
R4234	T14785	T14783	ccomp	shows,suggest
R4235	T14786	T14787	mark	although,resemble
R4236	T14655	T14652	nsubj	study,suggested
R4237	T14787	T14785	advcl	resemble,shows
R4238	T14788	T14787	nsubj	many,resemble
R4239	T14789	T14788	prep	of,many
R424	T2882	T2881	pobj	Kirby,of
R4240	T14656	T14655	amod	recent,study
R4241	T14790	T14791	det	the,phenotypes
R4242	T14791	T14789	pobj	phenotypes,of
R4243	T14792	T14791	amod	observed,phenotypes
R4244	T14657	T14658	advmod	in,vivo
R4245	T14793	T14788	acl	seen,many
R4246	T14794	T14793	prep	in,seen
R4247	T14658	T14655	amod	vivo,study
R4248	T14795	T14796	compound	Alk5,Cre
R4249	T14796	T14800	compound	Cre,mutants
R425	T2883	T2882	cc	and,Kirby
R4250	T14797	T14796	punct	/,Cre
R4251	T14659	T14660	mark	that,display
R4252	T14798	T14796	compound	Wnt1,Cre
R4253	T14799	T14796	punct	-,Cre
R4254	T14800	T14794	pobj	mutants,in
R4255	T14660	T14652	ccomp	display,suggested
R4256	T14801	T14787	advmod	superficially,resemble
R4257	T14802	T14787	dobj	those,resemble
R4258	T14661	T14660	nsubj	mice,display
R4259	T14803	T14802	acl	seen,those
R426	T2884	T2882	conj	coworkers,Kirby
R4260	T14804	T14803	prep	in,seen
R4261	T14805	T14806	compound	VCF,DGS
R4262	T14806	T14804	pobj	DGS,in
R4263	T14807	T14806	punct	/,DGS
R4264	T14808	T14785	punct	", ",shows
R4265	T14662	T14661	acl	lacking,mice
R4266	T14809	T14810	det	a,examination
R4267	T14810	T14785	nsubj	examination,shows
R4268	T14663	T14662	dobj	Tgfbr2,lacking
R4269	T14811	T14810	amod	detailed,examination
R427	T2943	T2933	cc	and,shown
R4270	T14812	T14813	mark	that,lead
R4271	T14664	T14663	prep	in,Tgfbr2
R4272	T14813	T14785	ccomp	lead,shows
R4273	T14814	T14815	det	the,abrogation
R4274	T14815	T14813	nsubj	abrogation,lead
R4275	T14665	T14664	pobj	CNCCs,in
R4276	T14816	T14817	npadvmod	NC,specific
R4277	T14817	T14815	amod	specific,abrogation
R4278	T14666	T14667	amod	defective,differentiation
R4279	T14818	T14817	punct	-,specific
R428	T2944	T2945	poss	their,fate
R4280	T14819	T14815	prep	of,abrogation
R4281	T14820	T14819	pobj	Alk5,of
R4282	T14667	T14660	dobj	differentiation,display
R4283	T14821	T14813	aux	does,lead
R4284	T14822	T14813	neg	not,lead
R4285	T14823	T14813	prep	to,lead
R4286	T14668	T14667	compound	NCC,differentiation
R4287	T14824	T14825	compound	VCF,DGS
R4288	T14825	T14827	npadvmod	DGS,like
R4289	T14669	T14660	prep	into,display
R429	T2885	T2886	punct	[,1
R4290	T14826	T14825	punct	/,DGS
R4291	T14827	T14829	amod	like,phenotypes
R4292	T14670	T14671	npadvmod	αSMA,positive
R4293	T14828	T14827	punct	-,like
R4294	T14671	T14673	amod	positive,cells
R4295	T14829	T14823	pobj	phenotypes,to
R4296	T14672	T14671	punct	-,positive
R4297	T14830	T14783	punct	.,suggest
R4298	T14673	T14669	pobj	cells,into
R4299	T14832	T14833	advmod	Firstly,seem
R43	T548	T546	pobj	defects,of
R430	T2945	T2946	nsubjpass	fate,controlled
R4300	T14674	T14660	prep	in,display
R4301	T14834	T14833	punct	", ",seem
R4302	T14835	T14836	mark	while,fails
R4303	T14675	T14676	det	the,septum
R4304	T14836	T14833	advcl	fails,seem
R4305	T14837	T14838	det	the,migration
R4306	T14838	T14836	nsubj	migration,fails
R4307	T14676	T14674	pobj	septum,in
R4308	T14839	T14840	amod	pharyngeal,organ
R4309	T14840	T14838	compound	organ,migration
R431	T2946	T2933	conj	controlled,shown
R4310	T14841	T14836	prep	in,fails
R4311	T14842	T14843	compound	Alk5,Cre
R4312	T14843	T14847	compound	Cre,mutants
R4313	T14844	T14843	punct	/,Cre
R4314	T14677	T14676	compound	AP,septum
R4315	T14845	T14843	compound	Wnt1,Cre
R4316	T14846	T14843	punct	-,Cre
R4317	T14847	T14841	pobj	mutants,in
R4318	T14889	T14887	acl	seen,death
R4319	T14848	T14836	punct	", ",fails
R432	T2947	T2946	auxpass	is,controlled
R4320	T14849	T14850	advmod	perhaps,as
R4321	T14850	T14836	prep	as,fails
R4322	T14890	T14889	prep	in,seen
R4323	T14851	T14852	det	a,result
R4324	T14852	T14850	pobj	result,as
R4325	T14853	T14852	prep	of,result
R4326	T14891	T14892	compound	Alk5,mutants
R4327	T14854	T14855	amod	increased,death
R4328	T14855	T14853	pobj	death,of
R4329	T14856	T14857	amod	mesenchymal,cell
R433	T2886	T2884	parataxis	1,coworkers
R4330	T14892	T14890	pobj	mutants,in
R4331	T14857	T14855	compound	cell,death
R4332	T14858	T14855	prep	in,death
R4333	T14859	T14860	det	the,region
R4334	T14893	T14894	det	a,population
R4335	T14860	T14858	pobj	region,in
R4336	T14861	T14860	amod	pharyngeal,region
R4337	T14894	T14884	dobj	population,affects
R4338	T14862	T14833	punct	", ",seem
R4339	T14863	T14864	preconj	both,thymus
R434	T2948	T2946	advmod	largely,controlled
R4340	T14864	T14833	nsubj	thymus,seem
R4341	T14895	T14896	advmod	predominantly,postmigratory
R4342	T14865	T14864	det	the,thymus
R4343	T14866	T14864	punct	", ",thymus
R4344	T14867	T14864	conj	thyroid,thymus
R4345	T14896	T14894	amod	postmigratory,population
R4346	T14868	T14867	cc	and,thyroid
R4347	T14869	T14867	conj	parathyroid,thyroid
R4348	T14897	T14894	prep	of,population
R4349	T14870	T14871	aux	to,develop
R435	T2949	T2946	agent	by,controlled
R4350	T14871	T14833	xcomp	develop,seem
R4351	T14872	T14873	advmod	relatively,normally
R4352	T14898	T14897	pobj	NCCs,of
R4353	T14873	T14871	advmod	normally,develop
R4354	T14874	T14871	prep	on,develop
R4355	T14875	T14876	det	the,level
R4356	T14899	T14884	punct	", ",affects
R4357	T14876	T14874	pobj	level,on
R4358	T14877	T14876	amod	histological,level
R4359	T14878	T14871	prep	in,develop
R436	T2887	T2886	punct	],1
R4360	T14900	T14901	mark	while,control
R4361	T14879	T14880	det	these,mutants
R4362	T14880	T14878	pobj	mutants,in
R4363	T14881	T14833	punct	.,seem
R4364	T14883	T14884	advmod	Secondly,affects
R4365	T14901	T14884	advcl	control,affects
R4366	T14885	T14884	punct	", ",affects
R4367	T14886	T14887	det	the,death
R4368	T14902	T14901	nsubj	genes,control
R4369	T14887	T14884	nsubj	death,affects
R437	T2950	T2951	amod	instructional,signals
R4370	T14888	T14887	compound	NCC,death
R4371	T14903	T14902	acl	located,genes
R4372	T14904	T14903	prep	in,located
R4373	T14905	T14906	det	the,DGCR
R4374	T14995	T14993	nsubj	migration,resulted
R4375	T14996	T14995	amod	defective,migration
R4376	T14906	T14904	pobj	DGCR,in
R4377	T14997	T14995	prep	of,migration
R4378	T14998	T14997	pobj	cells,of
R4379	T14999	T14995	prep	from,migration
R438	T2951	T2949	pobj	signals,by
R4380	T14907	T14906	punct	", ",DGCR
R4381	T14908	T14909	advmod	i.e.,Tbx1
R4382	T15000	T15001	det	the,field
R4383	T15001	T14999	pobj	field,from
R4384	T14909	T14906	appos	Tbx1,DGCR
R4385	T15002	T15001	amod	secondary,field
R4386	T15003	T15001	compound	heart,field
R4387	T14910	T14909	punct	", ",Tbx1
R4388	T15004	T14995	prep	to,migration
R4389	T15005	T15006	det	the,OFT
R439	T2888	T2882	punct	", ",Kirby
R4390	T15006	T15004	pobj	OFT,to
R4391	T14911	T14909	cc	and,Tbx1
R4392	T15007	T14993	prep	in,resulted
R4393	T15008	T15007	pobj	turn,in
R4394	T14912	T14909	conj	CrkL,Tbx1
R4395	T15009	T14993	prep	in,resulted
R4396	T15010	T15009	pobj	shortening,in
R4397	T14913	T14901	punct	", ",control
R4398	T15011	T15010	cc	and,shortening
R4399	T15012	T15013	amod	inappropriate,rotation
R44	T549	T543	prep	in,form
R440	T2952	T2951	prep	from,signals
R4400	T14914	T14915	compound	NCC,survival
R4401	T14915	T14901	dobj	survival,control
R4402	T14916	T14901	advmod	earlier,control
R4403	T15013	T15010	conj	rotation,shortening
R4404	T15014	T15010	prep	of,shortening
R4405	T14917	T14901	prep	at,control
R4406	T15015	T15016	det	the,OFT
R4407	T15016	T15014	pobj	OFT,of
R4408	T14918	T14917	pobj	E8.5,at
R4409	T15017	T14993	punct	", ",resulted
R441	T2953	T2954	amod	local,environments
R4410	T15018	T14993	advcl	leading,resulted
R4411	T15019	T15018	prep	to,leading
R4412	T14919	T14920	punct	-,E10
R4413	T15020	T15019	pobj	mal-alignment,to
R4414	T15021	T15020	prep	of,mal-alignment
R4415	T15022	T15023	det	the,pole
R4416	T15023	T15021	pobj	pole,of
R4417	T15024	T15023	amod	arterial,pole
R4418	T15025	T15020	prep	with,mal-alignment
R4419	T14920	T14918	prep	E10,E8.5
R442	T2889	T2890	dep	who,showed
R4420	T15026	T15027	det	the,ventricles
R4421	T15027	T15025	pobj	ventricles,with
R4422	T15028	T15029	punct	[,41
R4423	T14921	T14901	prep	by,control
R4424	T15029	T14993	parataxis	41,resulted
R4425	T15030	T15029	punct	],41
R4426	T15031	T14990	punct	.,suggested
R4427	T14922	T14921	pcomp	regulating,by
R4428	T15033	T15034	mark	While,shared
R4429	T15034	T15046	advcl	shared,suggest
R443	T2954	T2952	pobj	environments,from
R4430	T14923	T14922	dobj	proliferation,regulating
R4431	T15035	T15036	det	the,phenotype
R4432	T15036	T15034	nsubj	phenotype,shared
R4433	T14924	T14923	prep	of,proliferation
R4434	T15037	T15036	amod	detected,phenotype
R4435	T15038	T15036	compound	OFT,phenotype
R4436	T15039	T15036	prep	in,phenotype
R4437	T15040	T15041	compound	Alk5,Cre
R4438	T14925	T14926	det	the,field
R4439	T15041	T15045	compound	Cre,mutants
R444	T2955	T2956	prep	into,migrate
R4440	T15042	T15041	punct	/,Cre
R4441	T15043	T15041	compound	Wnt1,Cre
R4442	T14926	T14924	pobj	field,of
R4443	T15044	T15041	punct	-,Cre
R4444	T15045	T15039	pobj	mutants,in
R4445	T14927	T14926	amod	secondary,field
R4446	T15047	T15048	amod	many,similarities
R4447	T15048	T15034	dobj	similarities,shared
R4448	T14928	T14926	compound	heart,field
R4449	T15049	T15034	prep	with,shared
R445	T2956	T2954	relcl	migrate,environments
R4450	T15050	T15049	pobj	that,with
R4451	T15051	T15050	acl	seen,that
R4452	T14929	T14926	punct	(,field
R4453	T15052	T15051	prep	in,seen
R4454	T15053	T15054	det	the,models
R4455	T15054	T15052	pobj	models,in
R4456	T14930	T14926	appos	SHF,field
R4457	T15055	T15054	compound	chick,models
R4458	T14931	T14923	punct	),proliferation
R4459	T15056	T15054	compound	NC,models
R446	T2890	T2882	relcl	showed,Kirby
R4460	T15057	T15054	compound	ablation,models
R4461	T15058	T15054	punct	", ",models
R4462	T15059	T15060	advmod	e.g.,PTA
R4463	T14932	T14923	punct	", ",proliferation
R4464	T15060	T15054	appos	PTA,models
R4465	T15061	T15060	punct	", ",PTA
R4466	T15062	T15060	cc	and,PTA
R4467	T15063	T15064	det	the,sac
R4468	T15064	T15060	conj	sac,PTA
R4469	T14933	T14923	cc	and,proliferation
R447	T2957	T2955	pobj	which,into
R4470	T15065	T15064	amod	hypoplastic,sac
R4471	T15066	T15064	amod	aortic,sac
R4472	T15067	T15046	punct	", ",suggest
R4473	T14934	T14935	compound	endoderm,expansion
R4474	T15068	T15069	poss	our,results
R4475	T15069	T15046	nsubj	results,suggest
R4476	T15070	T15069	amod	current,results
R4477	T14935	T14923	conj	expansion,proliferation
R4478	T15071	T15072	mark	that,affected
R4479	T15072	T15046	ccomp	affected,suggest
R448	T2958	T2956	nsubj	NCCs,migrate
R4480	T15073	T15074	det	the,field
R4481	T14936	T14935	punct	", ",expansion
R4482	T15074	T15072	nsubjpass	field,affected
R4483	T15075	T15074	amod	secondary,field
R4484	T14937	T14938	dep	which,provides
R4485	T15076	T15074	compound	heart,field
R4486	T15077	T15072	auxpass	is,affected
R4487	T15078	T15072	neg	not,affected
R4488	T15079	T15072	advmod	severely,affected
R4489	T14938	T14935	relcl	provides,expansion
R449	T2959	T2960	punct	[,6
R4490	T15080	T15072	prep	in,affected
R4491	T15081	T15082	compound	Alk5,mutants
R4492	T14939	T14938	prep	in,provides
R4493	T14940	T14939	pobj	turn,in
R4494	T15082	T15080	pobj	mutants,in
R4495	T15083	T15084	punct	(,shown
R4496	T14941	T14942	compound	survival,signal
R4497	T15084	T15072	parataxis	shown,affected
R4498	T15085	T15084	nsubj	data,shown
R4499	T15086	T15084	neg	not,shown
R45	T550	T549	pobj	humans,in
R450	T2891	T2892	mark	that,led
R4500	T14942	T14938	dobj	signal,provides
R4501	T15087	T15084	punct	),shown
R4502	T15088	T15046	punct	.,suggest
R4503	T14943	T14942	prep	for,signal
R4504	T15090	T15091	mark	Since,detect
R4505	T15091	T15095	advcl	detect,appears
R4506	T14944	T14943	pobj	NCCs,for
R4507	T15092	T15091	nsubj	we,detect
R4508	T15093	T15091	aux	could,detect
R4509	T15094	T15091	neg	not,detect
R451	T2892	T2890	ccomp	led,showed
R4510	T14945	T14938	advcl	allowing,provides
R4511	T15096	T15097	amod	appropriate,rotation
R4512	T15097	T15091	dobj	rotation,detect
R4513	T14946	T14945	dobj	them,allowing
R4514	T15098	T15097	prep	of,rotation
R4515	T15099	T15100	det	the,OFT
R4516	T14947	T14948	aux	to,populate
R4517	T15100	T15098	pobj	OFT,of
R4518	T14948	T14945	xcomp	populate,allowing
R4519	T15101	T15091	prep	in,detect
R452	T2960	T2946	parataxis	6,controlled
R4520	T15102	T15103	amod	neural,crest
R4521	T14949	T14950	det	the,region
R4522	T15103	T15104	npadvmod	crest,specific
R4523	T15104	T15106	amod	specific,mutants
R4524	T15105	T15104	punct	-,specific
R4525	T14950	T14948	dobj	region,populate
R4526	T15106	T15101	pobj	mutants,in
R4527	T15107	T15106	prep	of,mutants
R4528	T15108	T15107	pobj	Alk5,of
R4529	T14951	T14950	amod	pharyngeal,region
R453	T2893	T2892	nsubj	ablation,led
R4530	T15109	T15108	cc	or,Alk5
R4531	T15110	T15108	conj	Alk2,Alk5
R4532	T14952	T14953	punct	[,36
R4533	T15111	T15095	punct	", ",appears
R4534	T15112	T15095	nsubj	it,appears
R4535	T15113	T15114	mark	that,required
R4536	T14953	T14938	parataxis	36,provides
R4537	T15114	T15095	ccomp	required,appears
R4538	T15115	T15114	nsubjpass	cells,required
R4539	T14954	T14955	punct	-,39
R454	T2961	T2960	punct	],6
R4540	T15116	T15115	acl	derived,cells
R4541	T15117	T15116	prep	from,derived
R4542	T15118	T15119	det	the,NC
R4543	T14955	T14953	prep	39,36
R4544	T15119	T15117	pobj	NC,from
R4545	T15120	T15115	punct	", ",cells
R4546	T15121	T15122	advmod	as,as
R4547	T14956	T14953	punct	],36
R4548	T15122	T15115	cc	as,cells
R4549	T15123	T15122	advmod	well,as
R455	T2962	T2946	punct	.,controlled
R4550	T15124	T15115	conj	those,cells
R4551	T14957	T14884	punct	.,affects
R4552	T15125	T15124	prep	from,those
R4553	T15126	T15127	det	the,SHF
R4554	T14959	T14960	compound	NCC,ablation
R4555	T15127	T15125	pobj	SHF,from
R4556	T15128	T15114	punct	", ",required
R4557	T15129	T15114	auxpass	are,required
R4558	T15130	T15114	advmod	mutually,required
R4559	T14960	T14961	nsubjpass	ablation,shown
R456	T2894	T2893	prep	of,ablation
R4560	T15131	T15114	prep	for,required
R4561	T15132	T15133	amod	proper,rotation
R4562	T15133	T15131	pobj	rotation,for
R4563	T14962	T14960	prep	in,ablation
R4564	T15134	T15133	compound	OFT,rotation
R4565	T15135	T15114	prep	in,required
R4566	T15136	T15135	pobj	mice,in
R4567	T14963	T14964	det	the,chick
R4568	T15137	T15095	punct	.,appears
R4569	T15139	T15140	advmod	However,appears
R457	T2964	T2965	amod	Several,studies
R4570	T14964	T14962	pobj	chick,in
R4571	T15141	T15140	punct	", ",appears
R4572	T15142	T15140	nsubj	it,appears
R4573	T15143	T15144	mark	that,contribute
R4574	T15144	T15140	ccomp	contribute,appears
R4575	T15145	T15146	det	these,receptors
R4576	T14965	T14961	aux	has,shown
R4577	T15146	T15144	nsubj	receptors,contribute
R4578	T15147	T15146	nummod	two,receptors
R4579	T15148	T15149	nmod	TGF,BMP
R458	T2965	T2967	nsubj	studies,indicated
R4580	T14966	T14961	auxpass	been,shown
R4581	T15149	T15146	nmod	BMP,receptors
R4582	T15150	T15149	punct	-,BMP
R4583	T15151	T15149	nmod	β,BMP
R4584	T14967	T14968	aux	to,lead
R4585	T15152	T15149	punct	/,BMP
R4586	T15153	T15146	nmod	type,receptors
R4587	T15154	T15153	nummod	I,type
R4588	T14968	T14961	xcomp	lead,shown
R4589	T15155	T15144	prep	to,contribute
R459	T2895	T2896	det	the,CNC
R4590	T15156	T15157	compound	OFT,rotation
R4591	T15157	T15155	pobj	rotation,to
R4592	T14969	T14968	prep	to,lead
R4593	T15158	T15144	prep	through,contribute
R4594	T15159	T15160	amod	different,mechanisms
R4595	T14970	T14969	pobj	PTA,to
R4596	T15160	T15158	pobj	mechanisms,through
R4597	T15161	T15140	punct	.,appears
R4598	T15163	T15164	prep	In,is
R4599	T14971	T14969	cc	and,to
R46	T551	T541	punct	.,are
R460	T2966	T2965	amod	recent,studies
R4600	T15165	T15166	compound	Alk5,mutants
R4601	T14972	T14969	conj	to,to
R4602	T15166	T15163	pobj	mutants,In
R4603	T15167	T15164	expl	there,is
R4604	T15168	T15169	advmod	very,little
R4605	T14973	T14974	det	a,failure
R4606	T14974	T14972	pobj	failure,to
R4607	T15169	T15170	amod	little,mesenchyme
R4608	T15170	T15164	attr	mesenchyme,is
R4609	T14975	T14974	prep	of,failure
R461	T2896	T2894	pobj	CNC,of
R4610	T15171	T15172	punct	", ",any
R4611	T15172	T15169	parataxis	any,little
R4612	T15173	T15172	mark	if,any
R4613	T14976	T14975	pobj	addition,of
R4614	T15174	T15172	punct	", ",any
R4615	T15175	T15170	amod	detectable,mesenchyme
R4616	T14977	T14976	prep	of,addition
R4617	T15176	T15170	amod	septal,mesenchyme
R4618	T15177	T15170	amod	present,mesenchyme
R4619	T14978	T14977	pobj	myocardium,of
R462	T2968	T2967	aux	have,indicated
R4620	T15178	T15164	punct	", ",is
R4621	T15179	T15164	cc	and,is
R4622	T14979	T14976	prep	from,addition
R4623	T15180	T15181	advmod	thus,argued
R4624	T15181	T15164	conj	argued,is
R4625	T15182	T15181	nsubjpass	it,argued
R4626	T15183	T15181	aux	could,argued
R4627	T15184	T15181	auxpass	be,argued
R4628	T14980	T14981	det	the,field
R4629	T15185	T15186	mark	that,fails
R463	T2969	T2970	mark	that,are
R4630	T15186	T15181	ccomp	fails,argued
R4631	T15187	T15186	prep	in,fails
R4632	T15188	T15189	det	these,mutants
R4633	T14981	T14979	pobj	field,from
R4634	T15189	T15187	pobj	mutants,in
R4635	T15190	T15191	compound	OFT,rotation
R4636	T14982	T14981	amod	secondary,field
R4637	T15191	T15186	nsubj	rotation,fails
R4638	T15192	T15186	prep	due,fails
R4639	T15193	T15192	pcomp	to,due
R464	T2970	T2967	ccomp	are,indicated
R4640	T14983	T14981	compound	heart,field
R4641	T15194	T15195	det	a,lack
R4642	T15195	T15192	pobj	lack,due
R4643	T15196	T15195	prep	of,lack
R4644	T14984	T14985	punct	[,40
R4645	T15197	T15196	pobj	penetration,of
R4646	T15198	T15197	prep	of,penetration
R4647	T15199	T15200	amod	septal,prongs
R4648	T14985	T14968	parataxis	40,lead
R4649	T15200	T15198	pobj	prongs,of
R465	T2897	T2893	prep	in,ablation
R4650	T15201	T15197	prep	into,penetration
R4651	T15202	T15203	det	the,mesenchyme
R4652	T14986	T14985	punct	],40
R4653	T15203	T15201	pobj	mesenchyme,into
R4654	T15204	T15203	compound	cushion,mesenchyme
R4655	T15205	T15181	punct	.,argued
R4656	T14987	T14961	punct	.,shown
R4657	T14989	T14990	nsubjpass	It,suggested
R4658	T14991	T14990	auxpass	was,suggested
R4659	T15207	T15208	prep	In,seen
R466	T2971	T2970	nsubj	members,are
R4660	T14992	T14993	mark	that,resulted
R4661	T15209	T15207	pobj	contrast,In
R4662	T15210	T15208	punct	", ",seen
R4663	T14993	T14990	ccomp	resulted,suggested
R4664	T15211	T15208	prep	in,seen
R4665	T15212	T15213	compound	Alk2,Cre
R4666	T15213	T15217	compound	Cre,mutants
R4667	T14994	T14995	det	the,migration
R4668	T15214	T15213	punct	/,Cre
R4669	T15215	T15213	compound	Wnt1,Cre
R467	T2972	T2971	prep	of,members
R4670	T15216	T15213	punct	-,Cre
R4671	T15217	T15211	pobj	mutants,in
R4672	T15218	T15219	det	a,mesenchyme
R4673	T15219	T15208	nsubjpass	mesenchyme,seen
R4674	T15220	T15219	amod	sizeable,mesenchyme
R4675	T15221	T15219	amod	septal,mesenchyme
R4676	T15222	T15208	aux	could,seen
R4677	T15223	T15208	auxpass	be,seen
R4678	T15224	T15208	punct	", ",seen
R4679	T15225	T15226	advmod	still,without
R468	T2973	T2974	det	the,superfamily
R4680	T15226	T15208	prep	without,seen
R4681	T15227	T15228	det	any,looping
R4682	T15228	T15226	pobj	looping,without
R4683	T15229	T15228	amod	obvious,looping
R4684	T15230	T15228	prep	of,looping
R4685	T15231	T15232	det	the,sac
R4686	T15232	T15230	pobj	sac,of
R4687	T15233	T15232	amod	aortic,sac
R4688	T15234	T15232	cc	and,sac
R4689	T15235	T15236	amod	truncal,OFT
R469	T2974	T2972	pobj	superfamily,of
R4690	T15236	T15232	conj	OFT,sac
R4691	T15237	T15208	punct	", ",seen
R4692	T15238	T15208	advcl	suggesting,seen
R4693	T15239	T15240	mark	that,is
R4694	T15240	T15238	ccomp	is,suggesting
R4695	T15241	T15242	det	the,presence
R4696	T15242	T15240	nsubj	presence,is
R4697	T15243	T15242	amod	mere,presence
R4698	T15244	T15242	prep	of,presence
R4699	T15245	T15246	det	the,mesenchyme
R47	T553	T554	det	A,portion
R470	T2975	T2976	compound	TGF,β
R4700	T15246	T15244	pobj	mesenchyme,of
R4701	T15247	T15246	amod	septal,mesenchyme
R4702	T15248	T15242	punct	", ",presence
R4703	T15249	T15242	prep	without,presence
R4704	T15250	T15251	amod	correct,differentiation
R4705	T15251	T15249	pobj	differentiation,without
R4706	T15252	T15253	amod	smooth,muscle
R4707	T15253	T15251	compound	muscle,differentiation
R4708	T15254	T15251	compound	cell,differentiation
R4709	T15255	T15240	punct	", ",is
R471	T2898	T2899	det	the,chick
R4710	T15256	T15240	neg	not,is
R4711	T15257	T15240	acomp	sufficient,is
R4712	T15258	T15257	prep	for,sufficient
R4713	T15259	T15260	compound	OFT,rotation
R4714	T15260	T15258	pobj	rotation,for
R4715	T15261	T15208	punct	.,seen
R472	T2976	T2974	compound	β,superfamily
R4720	T15539	T15540	prep	In,deleted
R4721	T15541	T15542	det	this,study
R4722	T15542	T15539	pobj	study,In
R4723	T15543	T15540	punct	", ",deleted
R4724	T15544	T15540	nsubj	we,deleted
R4725	T15545	T15540	aux	have,deleted
R4726	T15546	T15547	det	the,gene
R4727	T15547	T15540	dobj	gene,deleted
R4728	T15548	T15549	nmod	TGF,β
R4729	T15549	T15547	nmod	β,gene
R473	T2977	T2976	punct	-,β
R4730	T15550	T15549	punct	-,β
R4731	T15551	T15547	nmod	type,gene
R4732	T15552	T15551	nummod	I,type
R4733	T15553	T15547	nmod	receptor,gene
R4734	T15554	T15547	punct	(,gene
R4735	T15555	T15547	nmod	Alk5,gene
R4736	T15556	T15547	punct	),gene
R4737	T15557	T15540	advmod	specifically,deleted
R4738	T15558	T15540	prep	in,deleted
R4739	T15559	T15560	det	the,lineage
R474	T2978	T2971	punct	", ",members
R4740	T15560	T15558	pobj	lineage,in
R4741	T15561	T15560	nmod	mouse,lineage
R4742	T15562	T15563	amod	neural,crest
R4743	T15563	T15560	nmod	crest,lineage
R4744	T15564	T15563	punct	(,crest
R4745	T15565	T15563	appos	NC,crest
R4746	T15566	T15560	punct	),lineage
R4747	T15567	T15560	compound	cell,lineage
R4748	T15568	T15540	punct	.,deleted
R4749	T15570	T15571	poss	Our,data
R475	T2899	T2897	pobj	chick,in
R4750	T15571	T15572	nsubj	data,suggest
R4751	T15573	T15574	mark	that,required
R4752	T15574	T15572	ccomp	required,suggest
R4753	T15575	T15574	nsubjpass	ALK5,required
R4754	T15576	T15574	auxpass	is,required
R4755	T15577	T15578	npadvmod	cell,autonomously
R4756	T15578	T15574	advmod	autonomously,required
R4757	T15579	T15574	prep	in,required
R4758	T15580	T15581	det	the,NC
R4759	T15581	T15579	pobj	NC,in
R476	T2979	T2980	advmod	i.e.,βs
R4760	T15582	T15583	aux	to,mediate
R4761	T15583	T15574	advcl	mediate,required
R4762	T15584	T15585	amod	non-redundant,events
R4763	T15585	T15583	dobj	events,mediate
R4764	T15586	T15585	compound	signaling,events
R4765	T15587	T15588	dep	that,are
R4766	T15588	T15585	relcl	are,events
R4767	T15589	T15588	acomp	essential,are
R4768	T15590	T15589	prep	for,essential
R4769	T15591	T15592	amod	appropriate,patterning
R477	T2980	T2971	appos	βs,members
R4770	T15592	T15590	pobj	patterning,for
R4771	T15593	T15592	prep	of,patterning
R4772	T15594	T15595	det	the,organs
R4773	T15595	T15593	pobj	organs,of
R4774	T15596	T15595	amod	pharyngeal,organs
R4775	T15597	T15595	cc	and,organs
R4776	T15598	T15599	amod	cardiac,OFT
R4777	T15599	T15595	conj	OFT,organs
R4778	T15600	T15572	punct	.,suggest
R4779	T15602	T15603	det	The,defects
R478	T2900	T2892	prep	to,led
R4780	T15603	T15607	nsubj	defects,differ
R4781	T15604	T15603	amod	cardiac,defects
R4782	T15605	T15604	cc	and,cardiac
R4783	T15606	T15604	conj	pharyngeal,cardiac
R4784	T15608	T15603	acl	observed,defects
R4785	T15609	T15608	prep	in,observed
R4786	T15610	T15611	det	the,mutants
R4787	T15611	T15609	pobj	mutants,in
R4788	T15612	T15613	npadvmod	NC,specific
R4789	T15613	T15611	amod	specific,mutants
R479	T2981	T2980	punct	", ",βs
R4790	T15614	T15613	punct	-,specific
R4791	T15615	T15611	compound	Alk5,mutants
R4792	T15616	T15607	advmod	significantly,differ
R4793	T15617	T15607	prep	from,differ
R4794	T15618	T15617	pobj	those,from
R4795	T15619	T15618	acl	seen,those
R4796	T15620	T15619	prep	in,seen
R4797	T15621	T15622	amod	corresponding,mutants
R4798	T15622	T15620	pobj	mutants,in
R4799	T15623	T15622	acl	lacking,mutants
R48	T554	T556	nsubjpass	portion,associated
R480	T2982	T2980	compound	TGF,βs
R4800	T15624	T15625	det	the,receptor
R4801	T15625	T15623	dobj	receptor,lacking
R4802	T15626	T15627	nmod	TGF,β
R4803	T15627	T15625	nmod	β,receptor
R4804	T15628	T15627	punct	-,β
R4805	T15629	T15625	nmod	type,receptor
R4806	T15630	T15629	nummod	II,type
R4807	T15631	T15607	punct	", ",differ
R4808	T15632	T15607	advcl	suggesting,differ
R4809	T15633	T15634	mark	that,limited
R481	T2983	T2980	punct	-,βs
R4810	T15634	T15632	ccomp	limited,suggesting
R4811	T15635	T15634	nsubjpass	signaling,limited
R4812	T15636	T15635	acl	mediated,signaling
R4813	T15637	T15636	agent	by,mediated
R4814	T15638	T15637	pobj	ALK5,by
R4815	T15639	T15634	auxpass	is,limited
R4816	T15640	T15634	neg	not,limited
R4817	T15641	T15634	prep	to,limited
R4818	T15642	T15643	det	the,ligands
R4819	T15643	T15641	pobj	ligands,to
R482	T2901	T2902	amod	severe,tract
R4820	T15644	T15643	amod	classical,ligands
R4821	T15645	T15646	compound	TGF,β
R4822	T15646	T15643	compound	β,ligands
R4823	T15647	T15646	punct	-,β
R4824	T15648	T15634	prep	during,limited
R4825	T15649	T15650	amod	cardiac,pharyngeal
R4826	T15650	T15652	amod	pharyngeal,development
R4827	T15651	T15650	punct	/,pharyngeal
R4828	T15652	T15648	pobj	development,during
R4829	T15653	T15607	punct	.,differ
R483	T2984	T2980	cc	and,βs
R4830	T15914	T15915	compound	Alk5,Wnt1Cre
R4831	T15915	T15917	compound	Wnt1Cre,mice
R4832	T15916	T15915	punct	/,Wnt1Cre
R4833	T15919	T15920	nmod	Alk5,mutant
R4834	T15920	T15925	nmod	mutant,embryos
R4835	T15921	T15919	punct	(,Alk5
R4836	T15922	T15919	cc	and,Alk5
R4837	T15923	T15919	conj	Alk2,Alk5
R4838	T15924	T15920	punct	),mutant
R4839	T15925	T15928	nsubjpass	embryos,generated
R484	T2985	T2980	conj	BMPs,βs
R4840	T15926	T15920	cc	and,mutant
R4841	T15927	T15920	conj	control,mutant
R4842	T15929	T15928	auxpass	were,generated
R4843	T15930	T15928	agent	by,generated
R4844	T15931	T15930	pcomp	mating,by
R4845	T15932	T15933	nmod	Alk5ko,mice
R4846	T15933	T15931	dobj	mice,mating
R4847	T15934	T15932	punct	/,Alk5ko
R4848	T15935	T15932	punct	+,Alk5ko
R4849	T15936	T15937	punct	(,Alk2ko
R485	T2986	T2987	amod	likely,candidates
R4850	T15937	T15932	parataxis	Alk2ko,Alk5ko
R4851	T15938	T15937	punct	/,Alk2ko
R4852	T15939	T15937	punct	+,Alk2ko
R4853	T15940	T15937	punct	),Alk2ko
R4854	T15941	T15932	punct	/,Alk5ko
R4855	T15942	T15943	compound	Wnt1,Cre
R4856	T15943	T15932	appos	Cre,Alk5ko
R4857	T15944	T15943	punct	-,Cre
R4858	T15945	T15933	amod	male,mice
R4859	T15946	T15931	prep	with,mating
R486	T2902	T2900	pobj	tract,to
R4860	T15947	T15946	pobj	females,with
R4861	T15948	T15947	amod	homozygous,females
R4862	T15949	T15948	prep	for,homozygous
R4863	T15950	T15951	det	the,allele
R4864	T15951	T15949	pobj	allele,for
R4865	T15952	T15951	nmod	Alk5flox,allele
R4866	T15953	T15954	punct	(,Alk2flox
R4867	T15954	T15952	parataxis	Alk2flox,Alk5flox
R4868	T15955	T15954	punct	),Alk2flox
R4869	T15956	T15951	cc	and,allele
R487	T2987	T2970	attr	candidates,are
R4870	T15957	T15958	det	the,reporter
R4871	T15958	T15951	conj	reporter,allele
R4872	T15959	T15958	compound	R26R,reporter
R4873	T15960	T15961	punct	[,19
R4874	T15961	T15931	parataxis	19,mating
R4875	T15962	T15961	nummod	12,19
R4876	T15963	T15961	punct	",",19
R4877	T15964	T15961	punct	],19
R4878	T15965	T15928	punct	.,generated
R4879	T15967	T15968	nsubjpass	Genotyping,performed
R488	T2988	T2989	aux	to,provide
R4880	T15969	T15968	auxpass	was,performed
R4881	T15970	T15968	prep	by,performed
R4882	T15971	T15970	pobj	PCR,by
R4883	T15972	T15973	mark	as,described
R4884	T15973	T15968	advcl	described,performed
R4885	T15974	T15973	advmod	earlier,described
R4886	T15975	T15976	punct	[,42
R4887	T15976	T15973	parataxis	42,described
R4888	T15977	T15976	nummod	21,42
R4889	T15978	T15976	punct	",",42
R489	T2903	T2902	compound	outflow,tract
R4890	T15979	T15976	punct	],42
R4891	T15980	T15968	punct	.,performed
R4892	T15982	T15983	compound	Wnt1,Cre
R4893	T15983	T15985	compound	Cre,mice
R4894	T15984	T15983	punct	-,Cre
R4895	T15985	T15986	nsubjpass	mice,provided
R4896	T15987	T15986	auxpass	were,provided
R4897	T15988	T15986	advmod	kindly,provided
R4898	T15989	T15986	agent	by,provided
R4899	T15990	T15991	compound	A.,McMahon
R49	T555	T554	amod	substantial,portion
R490	T2989	T2987	advcl	provide,candidates
R4900	T15991	T15989	pobj	McMahon,by
R4901	T15992	T15993	punct	(,University
R4902	T15993	T15986	parataxis	University,provided
R4903	T15994	T15993	compound	Harvard,University
R4904	T15995	T15993	punct	),University
R4905	T15996	T15986	cc	and,provided
R4906	T15997	T15998	compound	R26R,reporter
R4907	T15998	T15999	compound	reporter,mice
R4908	T15999	T16000	nsubjpass	mice,obtained
R4909	T16000	T15986	conj	obtained,provided
R491	T2990	T2989	dobj	some,provide
R4910	T16001	T16000	auxpass	were,obtained
R4911	T16002	T16000	prep	from,obtained
R4912	T16003	T16004	det	the,laboratories
R4913	T16004	T16002	pobj	laboratories,from
R4914	T16005	T16004	compound	Jackson,laboratories
R4915	T16006	T16000	punct	.,obtained
R4916	T16008	T16009	det	All,studies
R4917	T16009	T16010	nsubjpass	studies,carried
R4918	T16011	T16010	auxpass	were,carried
R4919	T16012	T16010	prt	out,carried
R492	T2904	T2902	punct	(,tract
R4920	T16013	T16010	prep	at,carried
R4921	T16014	T16015	det	the,Facility
R4922	T16015	T16013	pobj	Facility,at
R4923	T16016	T16017	compound	Animal,Care
R4924	T16017	T16015	compound	Care,Facility
R4925	T16018	T16015	prep	of,Facility
R4926	T16019	T16020	det	the,Institute
R4927	T16020	T16018	pobj	Institute,of
R4928	T16021	T16020	compound	Saban,Institute
R4929	T16022	T16020	compound	Research,Institute
R493	T2991	T2990	prep	of,some
R4930	T16023	T16020	prep	of,Institute
R4931	T16024	T16025	compound	Childrens,Hospital
R4932	T16025	T16023	pobj	Hospital,of
R4933	T16026	T16027	compound	Los,Angeles
R4934	T16027	T16025	npadvmod	Angeles,Hospital
R4935	T16028	T16010	prep	in,carried
R4936	T16029	T16028	pobj	accordance,in
R4937	T16030	T16029	prep	with,accordance
R4938	T16031	T16032	amod	institutional,guidelines
R4939	T16032	T16030	pobj	guidelines,with
R494	T2992	T2993	det	these,signals
R4940	T16033	T16010	punct	.,carried
R4941	T16142	T16143	amod	Timed,mataings
R4942	T16145	T16146	nsubjpass	Mice,mated
R4943	T16146	T16148	ccomp	mated,designated
R4944	T16147	T16146	auxpass	were,mated
R4945	T16149	T16146	prep	during,mated
R4946	T16150	T16151	det	the,period
R4947	T16151	T16149	pobj	period,during
R4948	T16152	T16151	amod	dark,period
R4949	T16153	T16151	prep	of,period
R495	T2905	T2902	appos	OFT,tract
R4950	T16154	T16155	det	the,cycle
R4951	T16155	T16153	pobj	cycle,of
R4952	T16156	T16155	amod	controlled,cycle
R4953	T16157	T16155	compound	light,cycle
R4954	T16158	T16148	punct	;,designated
R4955	T16159	T16148	nsubjpass	presence,designated
R4956	T16160	T16159	prep	of,presence
R4957	T16161	T16162	amod	vaginal,plugs
R4958	T16162	T16160	pobj	plugs,of
R4959	T16163	T16148	auxpass	was,designated
R496	T2993	T2991	pobj	signals,of
R4960	T16164	T16148	prep	as,designated
R4961	T16165	T16164	pobj	day,as
R4962	T16166	T16165	nummod	0,day
R4963	T16167	T16165	npadvmod	hour,day
R4964	T16168	T16167	nummod	0,hour
R4965	T16169	T16148	punct	.,designated
R4966	T16171	T16172	nsubjpass	Females,euthanized
R4967	T16173	T16172	auxpass	were,euthanized
R4968	T16174	T16172	prep	by,euthanized
R4969	T16175	T16174	pobj	CO2,by
R497	T2994	T2967	punct	.,indicated
R4970	T16176	T16172	punct	", ",euthanized
R4971	T16177	T16172	cc	and,euthanized
R4972	T16178	T16179	nsubjpass	embryos,collected
R4973	T16179	T16172	conj	collected,euthanized
R4974	T16180	T16179	auxpass	were,collected
R4975	T16181	T16179	prep	in,collected
R4976	T16182	T16183	poss	Hanks,solution
R4977	T16183	T16181	pobj	solution,in
R4978	T16184	T16182	case	',Hanks
R4979	T16185	T16183	amod	balanced,solution
R498	T2906	T2902	punct	),tract
R4980	T16186	T16183	compound	salt,solution
R4981	T16187	T16183	prep	on,solution
R4982	T16188	T16187	pobj	ice,on
R4983	T16189	T16179	punct	.,collected
R4984	T16575	T16576	amod	Histological,analyses
R4985	T16578	T16579	nsubjpass	Embryos,fixed
R4986	T16580	T16581	punct	(,E17
R4987	T16581	T16578	parataxis	E17,Embryos
R4988	T16582	T16581	punct	),E17
R4989	T16583	T16579	auxpass	were,fixed
R499	T2996	T2997	nsubj	Mice,display
R4990	T16584	T16579	prep	with,fixed
R4991	T16585	T16586	nummod	4,%
R4992	T16586	T16587	compound	%,paraformaldehyhe
R4993	T16587	T16584	pobj	paraformaldehyhe,with
R4994	T16588	T16579	prep	for,fixed
R4995	T16589	T16590	nummod	14,hours
R4996	T16590	T16588	pobj	hours,for
R4997	T16591	T16579	punct	", ",fixed
R4998	T16592	T16579	conj	dehydrated,fixed
R4999	T16593	T16592	cc	and,dehydrated
R50	T557	T554	prep	of,portion
R500	T2907	T2902	nmod	defects,tract
R5000	T16594	T16592	conj	embedded,dehydrated
R5001	T16595	T16594	prep	in,embedded
R5002	T16596	T16597	compound	paraffin,wax
R5003	T16597	T16595	pobj	wax,in
R5004	T16598	T16579	punct	.,fixed
R5005	T16600	T16601	nsubjpass	Sections,stained
R5006	T16602	T16603	punct	(,um
R5007	T16603	T16600	parataxis	um,Sections
R5008	T16604	T16605	quantmod	7,8
R5009	T16605	T16603	nummod	8,um
R501	T2998	T2996	amod	deficient,Mice
R5010	T16606	T16605	punct	–,8
R5011	T16607	T16603	punct	),um
R5012	T16608	T16601	auxpass	were,stained
R5013	T16609	T16601	prep	with,stained
R5014	T16610	T16609	pobj	Hematoxylin,with
R5015	T16611	T16610	cc	and,Hematoxylin
R5016	T16612	T16610	conj	Eosin,Hematoxylin
R5017	T16613	T16614	punct	(,3
R5018	T16614	T16601	parataxis	3,stained
R5019	T16615	T16614	nsubj	n,3
R502	T2999	T2998	prep	in,deficient
R5020	T16616	T16614	punct	≥,3
R5021	T16617	T16614	prep	for,3
R5022	T16618	T16619	det	each,genotype
R5023	T16619	T16617	pobj	genotype,for
R5024	T16620	T16614	punct	),3
R5025	T16621	T16601	punct	.,stained
R5026	T16623	T16624	prep	For,stained
R5027	T16625	T16626	compound	lineage,tracing
R5028	T16626	T16627	compound	tracing,analyses
R5029	T16627	T16623	pobj	analyses,For
R503	T3000	T3001	compound	TGF,β2
R5030	T16628	T16624	punct	", ",stained
R5031	T16629	T16624	nsubjpass	embryos,stained
R5032	T16630	T16624	auxpass	were,stained
R5033	T16631	T16624	prep	for,stained
R5034	T16632	T16633	compound	β,galactosidase
R5035	T16633	T16635	compound	galactosidase,activity
R5036	T16634	T16633	punct	-,galactosidase
R5037	T16635	T16631	pobj	activity,for
R5038	T16636	T16637	mark	as,described
R5039	T16637	T16624	advcl	described,stained
R504	T2908	T2902	prep	including,tract
R5040	T16638	T16639	punct	[,43
R5041	T16639	T16624	parataxis	43,stained
R5042	T16640	T16639	punct	],43
R5043	T16641	T16624	punct	.,stained
R5044	T16643	T16644	advmod	Briefly,fixed
R5045	T16645	T16644	punct	", ",fixed
R5046	T16646	T16647	det	the,specimens
R5047	T16647	T16644	nsubjpass	specimens,fixed
R5048	T16648	T16649	punct	(,E11.0
R5049	T16649	T16647	parataxis	E11.0,specimens
R505	T3001	T2999	pobj	β2,in
R5050	T16650	T16651	punct	–,E11.5
R5051	T16651	T16649	prep	E11.5,E11.0
R5052	T16652	T16649	punct	),E11.0
R5053	T16653	T16644	auxpass	were,fixed
R5054	T16654	T16644	prep	in,fixed
R5055	T16655	T16656	nummod	4,%
R5056	T16656	T16657	compound	%,paraformaldehyde
R5057	T16657	T16654	pobj	paraformaldehyde,in
R5058	T16658	T16644	prep	for,fixed
R5059	T16659	T16660	nummod	30,minutes
R506	T3002	T3001	punct	-,β2
R5060	T16660	T16658	pobj	minutes,for
R5061	T16661	T16644	prep	at,fixed
R5062	T16662	T16663	compound	room,temperature
R5063	T16663	T16661	pobj	temperature,at
R5064	T16664	T16644	punct	", ",fixed
R5065	T16665	T16644	conj	washed,fixed
R5066	T16666	T16667	nummod	3,times
R5067	T16667	T16665	npadvmod	times,washed
R5068	T16668	T16665	prep	for,washed
R5069	T16669	T16670	nummod	10,minutes
R507	T3003	T3004	amod	fourth,defects
R5070	T16670	T16668	pobj	minutes,for
R5071	T16671	T16665	prep	in,washed
R5072	T16672	T16673	det	the,wash
R5073	T16673	T16671	pobj	wash,in
R5074	T16674	T16673	compound	detergent,wash
R5075	T16675	T16665	cc	and,washed
R5076	T16676	T16665	conj	developed,washed
R5077	T16677	T16676	prep	for,developed
R5078	T16678	T16679	quantmod	2,12
R5079	T16679	T16681	nummod	12,hours
R508	T2909	T2910	amod	persistent,arteriosus
R5080	T16680	T16679	punct	–,12
R5081	T16681	T16677	pobj	hours,for
R5082	T16682	T16676	prep	in,developed
R5083	T16683	T16684	compound	X,gal
R5084	T16684	T16686	compound	gal,solution
R5085	T16685	T16684	punct	-,gal
R5086	T16686	T16682	pobj	solution,in
R5087	T16687	T16688	punct	(,3
R5088	T16688	T16676	parataxis	3,developed
R5089	T16689	T16688	nsubj	n,3
R509	T3004	T2997	dobj	defects,display
R5090	T16690	T16688	punct	≥,3
R5091	T16691	T16688	prep	for,3
R5092	T16692	T16693	det	each,genotype
R5093	T16693	T16691	pobj	genotype,for
R5094	T16694	T16688	punct	),3
R5095	T16695	T16644	punct	.,fixed
R5096	T16697	T16698	prep	For,stained
R5097	T16699	T16697	pobj	immunohistochemistry,For
R5098	T16700	T16698	punct	", ",stained
R5099	T16701	T16702	amod	fixed,sections
R51	T558	T559	det	these,defects
R510	T3005	T3006	amod	aortic,artery
R5100	T16702	T16698	nsubjpass	sections,stained
R5101	T16703	T16702	prep	from,sections
R5102	T16704	T16703	pobj	tissues,from
R5103	T16705	T16704	acl	harvested,tissues
R5104	T16706	T16705	prep	at,harvested
R5105	T16707	T16706	pobj	E10.5,at
R5106	T16708	T16709	punct	–,E11.5
R5107	T16709	T16707	prep	E11.5,E10.5
R5108	T16710	T16698	auxpass	were,stained
R5109	T16711	T16698	prep	with,stained
R511	T3006	T3004	compound	artery,defects
R5110	T16712	T16713	amod	monoclonal,actin
R5111	T16713	T16718	nmod	actin,antibodies
R5112	T16714	T16715	nmod	α,muscle
R5113	T16715	T16713	nmod	muscle,actin
R5114	T16716	T16715	punct	-,muscle
R5115	T16717	T16715	amod	smooth,muscle
R5116	T16718	T16711	pobj	antibodies,with
R5117	T16719	T16720	punct	(,DAKO
R5118	T16720	T16713	parataxis	DAKO,actin
R5119	T16721	T16720	punct	),DAKO
R512	T2910	T2908	pobj	arteriosus,including
R5120	T16722	T16713	punct	", ",actin
R5121	T16723	T16724	amod	cleaved,caspase
R5122	T16724	T16713	conj	caspase,actin
R5123	T16725	T16724	punct	-,caspase
R5124	T16726	T16724	nummod	3,caspase
R5125	T16727	T16728	punct	(,Signaling
R5126	T16728	T16724	parataxis	Signaling,caspase
R5127	T16729	T16728	compound	Cell,Signaling
R5128	T16730	T16728	punct	),Signaling
R5129	T16731	T16724	cc	or,caspase
R513	T3007	T3006	compound	arch,artery
R5130	T16732	T16733	compound	phophohistone,H3
R5131	T16733	T16724	conj	H3,caspase
R5132	T16734	T16733	punct	-,H3
R5133	T16735	T16736	punct	(,Signaling
R5134	T16736	T16733	parataxis	Signaling,H3
R5135	T16737	T16736	compound	Cell,Signaling
R5136	T16738	T16736	punct	),Signaling
R5137	T16739	T16698	punct	.,stained
R5138	T16741	T16742	compound	TUNEL,assays
R5139	T16742	T16743	nsubjpass	assays,performed
R514	T3008	T3009	punct	[,7
R5140	T16744	T16743	auxpass	were,performed
R5141	T16745	T16743	advcl	using,performed
R5142	T16746	T16747	det	the,system
R5143	T16747	T16745	dobj	system,using
R5144	T16748	T16749	nmod	DeadEnd,TUNEL
R5145	T16749	T16747	compound	TUNEL,system
R5146	T16750	T16749	amod	fluorometric,TUNEL
R5147	T16751	T16752	punct	(,Promega
R5148	T16752	T16745	parataxis	Promega,using
R5149	T16753	T16752	punct	),Promega
R515	T3009	T2997	parataxis	7,display
R5150	T16754	T16743	punct	.,performed
R5151	T16756	T16757	prep	In,analyzed
R5152	T16758	T16759	det	each,assay
R5153	T16759	T16756	pobj	assay,In
R5154	T16760	T16761	nummod	3,embryos
R5155	T16761	T16757	nsubjpass	embryos,analyzed
R5156	T16762	T16760	cc	or,3
R5157	T16763	T16760	conj	more,3
R5158	T16764	T16757	auxpass	were,analyzed
R5159	T16765	T16757	prep	for,analyzed
R516	T2911	T2910	compound	truncus,arteriosus
R5160	T16766	T16767	det	each,genotype
R5161	T16767	T16765	pobj	genotype,for
R5162	T16768	T16757	punct	.,analyzed
R5163	T16958	T16959	nmod	Ink,injections
R5164	T16960	T16958	cc	and,Ink
R5165	T16961	T16962	compound	casting,dye
R5166	T16962	T16958	conj	dye,Ink
R5167	T16964	T16965	nsubjpass	Embryos,dissected
R5168	T16966	T16967	punct	(,E10.0
R5169	T16967	T16964	parataxis	E10.0,Embryos
R517	T3010	T3009	punct	],7
R5170	T16968	T16969	punct	–,E13.0
R5171	T16969	T16967	prep	E13.0,E10.0
R5172	T16970	T16967	punct	),E10.0
R5173	T16971	T16965	auxpass	were,dissected
R5174	T16972	T16965	cc	and,dissected
R5175	T16973	T16965	conj	placed,dissected
R5176	T16974	T16973	prep	in,placed
R5177	T16975	T16976	nmod	ice,PBS
R5178	T16976	T16974	pobj	PBS,in
R5179	T16977	T16976	amod	cold,PBS
R518	T3011	T2997	punct	", ",display
R5180	T16978	T16979	punct	(,3
R5181	T16979	T16973	parataxis	3,placed
R5182	T16980	T16979	nsubj	n,3
R5183	T16981	T16979	punct	≥,3
R5184	T16982	T16979	prep	per,3
R5185	T16983	T16982	pobj	genotype,per
R5186	T16984	T16979	prep	in,3
R5187	T16985	T16986	det	each,point
R5188	T16986	T16984	pobj	point,in
R5189	T16987	T16986	compound	time,point
R519	T2912	T2910	punct	(,arteriosus
R5190	T16988	T16979	punct	),3
R5191	T16989	T16965	punct	.,dissected
R5192	T16991	T16992	advcl	Using,injected
R5193	T16993	T16994	det	a,pipette
R5194	T16994	T16991	dobj	pipette,Using
R5195	T16995	T16994	amod	pulled,pipette
R5196	T16996	T16994	compound	glass,pipette
R5197	T16997	T16992	punct	", ",injected
R5198	T16998	T16999	compound	India,ink
R5199	T16999	T16992	nsubjpass	ink,injected
R52	T559	T557	pobj	defects,of
R520	T3012	T3013	mark	while,results
R5200	T17000	T16999	cc	or,ink
R5201	T17001	T17002	amod	Yellow,dye
R5202	T17002	T16999	conj	dye,ink
R5203	T17003	T17002	compound	casting,dye
R5204	T17004	T17005	punct	(,Supply
R5205	T17005	T17002	parataxis	Supply,dye
R5206	T17006	T17007	compound	Connecticut,Valley
R5207	T17007	T17005	compound	Valley,Supply
R5208	T17008	T17005	compound	Biological,Supply
R5209	T17009	T17005	punct	),Supply
R521	T3013	T2997	advcl	results,display
R5210	T17010	T16992	auxpass	was,injected
R5211	T17011	T16992	prep	into,injected
R5212	T17012	T17013	det	the,ventricles
R5213	T17013	T17011	pobj	ventricles,into
R5214	T17014	T17015	mark	until,penetrated
R5215	T17015	T16992	advcl	penetrated,injected
R5216	T17016	T17017	compound	ink,dye
R5217	T17017	T17015	nsubj	dye,penetrated
R5218	T17018	T17017	punct	/,dye
R5219	T17019	T17020	amod	small,vessels
R522	T3014	T3015	amod	neural,cell
R5220	T17020	T17015	dobj	vessels,penetrated
R5221	T17021	T16992	punct	.,injected
R5222	T17023	T17024	nsubjpass	Embryos,postfixed
R5223	T17025	T17024	auxpass	were,postfixed
R5224	T17026	T17024	prep	in,postfixed
R5225	T17027	T17028	nummod	10,%
R5226	T17028	T17029	nmod	%,formalin
R5227	T17029	T17026	pobj	formalin,in
R5228	T17030	T17029	amod	buffered,formalin
R5229	T17031	T17024	prep	for,postfixed
R523	T3015	T3017	npadvmod	cell,specific
R5230	T17032	T17033	nummod	12,hours
R5231	T17033	T17031	pobj	hours,for
R5232	T17034	T17024	punct	", ",postfixed
R5233	T17035	T17024	conj	dehydrated,postfixed
R5234	T17036	T17035	cc	and,dehydrated
R5235	T17037	T17035	conj	cleared,dehydrated
R5236	T17038	T17037	prep	in,cleared
R5237	T17039	T17038	pobj	benzylbenzoate,in
R5238	T17040	T17039	punct	: ,benzylbenzoate
R5239	T17041	T17042	compound	benzyl,alcohol
R524	T3016	T3015	compound	crest,cell
R5240	T17042	T17039	appos	alcohol,benzylbenzoate
R5241	T17043	T17044	punct	(,2
R5242	T17044	T17039	parataxis	2,benzylbenzoate
R5243	T17045	T17046	punct	:,v
R5244	T17046	T17044	prep	v,2
R5245	T17047	T17046	compound	1v,v
R5246	T17048	T17046	punct	/,v
R5247	T17049	T17044	punct	),2
R5248	T17050	T17024	punct	.,postfixed
R5249	T17140	T17141	compound	Expression,analyses
R525	T3017	T3018	amod	specific,abrogation
R5250	T17143	T17144	aux	To,visualize
R5251	T17144	T17145	advcl	visualize,used
R5252	T17146	T17147	compound	parathyroid,hormone
R5253	T17147	T17148	compound	hormone,expression
R5254	T17148	T17144	dobj	expression,visualize
R5255	T17149	T17145	nsubj	we,used
R5256	T17150	T17151	nmod	in,situ
R5257	T17151	T17152	nmod	situ,hybridization
R5258	T17152	T17145	dobj	hybridization,used
R5259	T17153	T17152	cc	and,hybridization
R526	T2913	T2910	appos	PTA,arteriosus
R5260	T17154	T17155	det	an,probe
R5261	T17155	T17152	conj	probe,hybridization
R5262	T17156	T17155	compound	antisense,probe
R5263	T17157	T17155	acl	corresponding,probe
R5264	T17158	T17157	prep	to,corresponding
R5265	T17159	T17158	pobj	nucleotides,to
R5266	T17160	T17161	quantmod	97,534
R5267	T17161	T17159	nummod	534,nucleotides
R5268	T17162	T17161	punct	–,534
R5269	T17163	T17159	punct	(,nucleotides
R527	T3018	T3013	nsubj	abrogation,results
R5270	T17164	T17165	advmod	kindly,provided
R5271	T17165	T17159	acl	provided,nucleotides
R5272	T17166	T17165	agent	by,provided
R5273	T17167	T17168	compound	Nancy,Manley
R5274	T17168	T17166	pobj	Manley,by
R5275	T17169	T17145	punct	),used
R5276	T17170	T17171	mark	as,described
R5277	T17171	T17145	advcl	described,used
R5278	T17172	T17173	punct	[,44
R5279	T17173	T17171	parataxis	44,described
R528	T3019	T3018	prep	of,abrogation
R5280	T17174	T17173	punct	],44
R5281	T17175	T17145	punct	.,used
R5282	T17691	T17692	nsubj	Abrogation,leads
R5283	T17693	T17691	prep	of,Abrogation
R5284	T17694	T17693	pobj	Alk5,of
R5285	T17695	T17691	prep	in,Abrogation
R5286	T17696	T17697	amod	neural,cells
R5287	T17697	T17695	pobj	cells,in
R5288	T17698	T17697	compound	crest,cells
R5289	T17699	T17692	prep	to,leads
R529	T3020	T3021	nmod	TGF,β
R5290	T17700	T17701	amod	persistent,arteriosus
R5291	T17701	T17699	pobj	arteriosus,to
R5292	T17702	T17701	compound	truncus,arteriosus
R5293	T17703	T17704	compound	type,A2
R5294	T17704	T17701	npadvmod	A2,arteriosus
R5295	T17705	T17692	punct	.,leads
R5296	T17707	T17708	meta	A,analysis
R5297	T17709	T17707	punct	-,A
R5298	T17710	T17707	dep	D,A
R5299	T17711	T17708	punct	", ",analysis
R53	T560	T556	aux	has,associated
R530	T2914	T2902	punct	),tract
R5300	T17712	T17713	amod	Casting,dye
R5301	T17713	T17708	compound	dye,analysis
R5302	T17714	T17713	punct	-,dye
R5303	T17715	T17708	prep	of,analysis
R5304	T17716	T17717	compound	OFT,morphogenesis
R5305	T17717	T17715	pobj	morphogenesis,of
R5306	T17718	T17708	prep	at,analysis
R5307	T17719	T17718	pobj	E17.0,at
R5308	T17720	T17708	punct	.,analysis
R5309	T17722	T17723	nmod	Control,mutant
R531	T3021	T3023	nmod	β,receptor
R5310	T17724	T17725	punct	(,A
R5311	T17725	T17722	parataxis	A,Control
R5312	T17726	T17725	punct	),A
R5313	T17727	T17723	punct	", ",mutant
R5314	T17728	T17729	compound	Tgfbr2,Cre
R5315	T17729	T17723	compound	Cre,mutant
R5316	T17730	T17729	punct	/,Cre
R5317	T17731	T17729	compound	Wnt1,Cre
R5318	T17732	T17729	punct	-,Cre
R5319	T17733	T17734	punct	[,8
R532	T3022	T3021	punct	-,β
R5320	T17734	T17723	parataxis	8,mutant
R5321	T17735	T17734	punct	],8
R5322	T17736	T17737	punct	(,B
R5323	T17737	T17723	parataxis	B,mutant
R5324	T17738	T17737	punct	),B
R5325	T17739	T17723	acl	demonstrating,mutant
R5326	T17740	T17741	det	the,A4
R5327	T17741	T17739	dobj	A4,demonstrating
R5328	T17742	T17741	compound	PTA,A4
R5329	T17743	T17741	compound	type,A4
R533	T2915	T2902	punct	", ",tract
R5330	T17744	T17741	punct	(,A4
R5331	T17745	T17746	punct	=,arteriosus
R5332	T17746	T17741	acl	arteriosus,A4
R5333	T17747	T17746	compound	truncus,arteriosus
R5334	T17748	T17746	prep	with,arteriosus
R5335	T17749	T17750	amod	interrupted,arch
R5336	T17750	T17748	pobj	arch,with
R5337	T17751	T17750	amod	aortic,arch
R5338	T17752	T17753	punct	[,30
R5339	T17753	T17746	parataxis	30,arteriosus
R534	T3023	T3019	pobj	receptor,of
R5340	T17754	T17753	punct	],30
R5341	T17755	T17723	punct	),mutant
R5342	T17756	T17723	cc	and,mutant
R5343	T17757	T17758	compound	Alk5,Cre
R5344	T17758	T17762	compound	Cre,mutants
R5345	T17759	T17758	punct	/,Cre
R5346	T17760	T17758	compound	Wnt1,Cre
R5347	T17761	T17758	punct	-,Cre
R5348	T17762	T17723	conj	mutants,mutant
R5349	T17763	T17762	acl	demonstrating,mutants
R535	T3024	T3023	nmod	type,receptor
R5350	T17764	T17765	det	the,arches
R5351	T17765	T17763	dobj	arches,demonstrating
R5352	T17766	T17767	amod	right,sided
R5353	T17767	T17765	amod	sided,arches
R5354	T17768	T17767	punct	-,sided
R5355	T17769	T17770	punct	(,C
R5356	T17770	T17767	parataxis	C,sided
R5357	T17771	T17770	punct	),C
R5358	T17772	T17767	cc	and,sided
R5359	T17773	T17774	amod	left,sided
R536	T2916	T2902	conj	mispatterning,tract
R5360	T17774	T17767	conj	sided,sided
R5361	T17775	T17774	punct	-,sided
R5362	T17776	T17777	punct	(,D
R5363	T17777	T17774	parataxis	D,sided
R5364	T17778	T17777	punct	),D
R5365	T17779	T17765	prep	of,arches
R5366	T17780	T17781	det	the,truncus
R5367	T17781	T17779	pobj	truncus,of
R5368	T17782	T17762	punct	.,mutants
R5369	T17784	T17785	meta	E,cross-sections
R537	T3025	T3023	nummod	II,receptor
R5370	T17786	T17784	punct	-,E
R5371	T17787	T17784	dep	P,E
R5372	T17788	T17785	punct	", ",cross-sections
R5373	T17789	T17785	amod	Histological,cross-sections
R5374	T17790	T17785	prep	on,cross-sections
R5375	T17791	T17792	nummod	four,levels
R5376	T17792	T17790	pobj	levels,on
R5377	T17793	T17792	amod	different,levels
R5378	T17794	T17795	punct	(,rostral
R5379	T17795	T17792	parataxis	rostral,levels
R538	T3026	T3023	punct	(,receptor
R5380	T17796	T17795	prep	to,rostral
R5381	T17797	T17796	amod	caudal,to
R5382	T17798	T17795	punct	),rostral
R5383	T17799	T17785	prep	at,cross-sections
R5384	T17800	T17799	pobj	E17.0,at
R5385	T17801	T17785	punct	.,cross-sections
R5386	T17803	T17804	prep	In,separated
R5387	T17805	T17806	det	a,control
R5388	T17806	T17803	pobj	control,In
R5389	T17807	T17808	punct	(,E
R539	T3027	T3023	appos	Tgfbr2,receptor
R5390	T17808	T17806	parataxis	E,control
R5391	T17809	T17810	punct	-,H
R5392	T17810	T17808	prep	H,E
R5393	T17811	T17808	punct	),E
R5394	T17812	T17804	punct	", ",separated
R5395	T17813	T17814	det	the,aorta
R5396	T17814	T17804	nsubjpass	aorta,separated
R5397	T17815	T17814	amod	ascending,aorta
R5398	T17816	T17814	punct	(,aorta
R5399	T17817	T17814	appos	Ao,aorta
R54	T561	T556	auxpass	been,associated
R540	T2917	T2916	prep	of,mispatterning
R5400	T17818	T17814	punct	),aorta
R5401	T17819	T17814	cc	and,aorta
R5402	T17820	T17821	amod	pulmonary,trunk
R5403	T17821	T17814	conj	trunk,aorta
R5404	T17822	T17821	punct	(,trunk
R5405	T17823	T17821	appos	PT,trunk
R5406	T17824	T17804	punct	),separated
R5407	T17825	T17804	auxpass	are,separated
R5408	T17826	T17804	agent	by,separated
R5409	T17827	T17828	det	the,septum
R541	T3028	T3013	punct	),results
R5410	T17828	T17826	pobj	septum,by
R5411	T17829	T17828	amod	conotruncal,septum
R5412	T17830	T17804	punct	.,separated
R5413	T17832	T17833	prep	In,fails
R5414	T17834	T17835	compound	Alk5,Cre
R5415	T17835	T17839	compound	Cre,mutants
R5416	T17836	T17835	punct	/,Cre
R5417	T17837	T17835	compound	Wnt1,Cre
R5418	T17838	T17835	punct	-,Cre
R5419	T17839	T17832	pobj	mutants,In
R542	T3029	T3013	prep	in,results
R5420	T17840	T17841	punct	(,I
R5421	T17841	T17839	parataxis	I,mutants
R5422	T17842	T17843	punct	-,P
R5423	T17843	T17841	prep	P,I
R5424	T17844	T17841	punct	),I
R5425	T17845	T17846	det	the,septum
R5426	T17846	T17833	nsubj	septum,fails
R5427	T17847	T17846	amod	conotruncal,septum
R5428	T17848	T17849	aux	to,form
R5429	T17849	T17833	xcomp	form,fails
R543	T3030	T3029	pobj	interruption,in
R5430	T17850	T17833	punct	", ",fails
R5431	T17851	T17833	cc	and,fails
R5432	T17852	T17853	preconj	either,sided
R5433	T17853	T17856	amod	sided,arch
R5434	T17854	T17853	amod	left,sided
R5435	T17855	T17853	punct	-,sided
R5436	T17856	T17872	nsubjpass	arch,seen
R5437	T17857	T17858	punct	(,I
R5438	T17858	T17853	parataxis	I,sided
R5439	T17859	T17860	punct	-,L
R544	T2918	T2919	det	the,vessels
R5440	T17860	T17858	prep	L,I
R5441	T17861	T17858	punct	),I
R5442	T17862	T17853	cc	or,sided
R5443	T17863	T17864	amod	right,sided
R5444	T17864	T17853	conj	sided,sided
R5445	T17865	T17864	punct	-,sided
R5446	T17866	T17867	punct	(,M
R5447	T17867	T17864	parataxis	M,sided
R5448	T17868	T17869	punct	-,P
R5449	T17869	T17867	prep	P,M
R545	T3031	T3030	prep	of,interruption
R5450	T17870	T17867	punct	),M
R5451	T17871	T17856	amod	aortic,arch
R5452	T17872	T17833	conj	seen,fails
R5453	T17873	T17872	aux	can,seen
R5454	T17874	T17872	auxpass	be,seen
R5455	T17875	T17833	punct	.,fails
R5456	T17877	T17878	amod	Aberrant,branching
R5457	T17878	T17879	nsubjpass	branching,illustrated
R5458	T17880	T17878	prep	of,branching
R5459	T17881	T17882	amod	carotid,arteries
R546	T2919	T2917	pobj	vessels,of
R5460	T17882	T17880	pobj	arteries,of
R5461	T17883	T17882	prep	from,arteries
R5462	T17884	T17885	det	the,truncus
R5463	T17885	T17883	pobj	truncus,from
R5464	T17886	T17879	aux	has,illustrated
R5465	T17887	T17879	auxpass	been,illustrated
R5466	T17888	T17879	prep	by,illustrated
R5467	T17889	T17890	amod	black,arrows
R5468	T17890	T17888	pobj	arrows,by
R5469	T17891	T17892	punct	(,J
R547	T3032	T3033	det	the,arch
R5470	T17892	T17879	parataxis	J,illustrated
R5471	T17893	T17892	cc	and,J
R5472	T17894	T17892	conj	M,J
R5473	T17895	T17892	punct	),J
R5474	T17896	T17879	punct	.,illustrated
R5475	T17899	T17898	punct	", ",Ao
R5476	T17900	T17898	appos	aorta,Ao
R5477	T17901	T17898	punct	;,Ao
R5478	T17902	T17898	appos	PT,Ao
R5479	T17903	T17902	punct	", ",PT
R548	T3033	T3031	pobj	arch,of
R5480	T17904	T17905	amod	pulmonary,trunk
R5481	T17905	T17902	appos	trunk,PT
R5482	T17906	T17898	punct	;,Ao
R5483	T17907	T17898	appos	RSA,Ao
R5484	T17908	T17907	punct	", ",RSA
R5485	T17909	T17910	amod	right,artery
R5486	T17910	T17907	appos	artery,RSA
R5487	T17911	T17910	amod	subclavian,artery
R5488	T17912	T17898	punct	;,Ao
R5489	T17913	T17898	appos	RCA,Ao
R549	T3034	T3033	amod	aortic,arch
R5490	T17914	T17913	punct	", ",RCA
R5491	T17915	T17916	amod	right,artery
R5492	T17916	T17913	appos	artery,RCA
R5493	T17917	T17916	compound	carotid,artery
R5494	T17918	T17898	punct	;,Ao
R5495	T17919	T17898	appos	LCA,Ao
R5496	T17920	T17919	punct	;,LCA
R5497	T17921	T17922	amod	left,artery
R5498	T17922	T17919	appos	artery,LCA
R5499	T17923	T17922	amod	carotid,artery
R55	T562	T556	prep	with,associated
R550	T2920	T2919	amod	great,vessels
R5500	T17924	T17898	punct	;,Ao
R5501	T17925	T17898	appos	LSA,Ao
R5502	T17926	T17925	punct	", ",LSA
R5503	T17927	T17928	amod	left,artery
R5504	T17928	T17925	appos	artery,LSA
R5505	T17929	T17928	amod	subclavian,artery
R5506	T17930	T17898	punct	;,Ao
R5507	T17931	T17898	appos	IAA,Ao
R5508	T17932	T17931	punct	", ",IAA
R5509	T17933	T17934	amod	interrupted,arch
R551	T3035	T3033	cc	and,arch
R5510	T17934	T17931	appos	arch,IAA
R5511	T17935	T17934	amod	aortic,arch
R5512	T17936	T17898	punct	;,Ao
R5513	T17937	T17898	appos	PTA,Ao
R5514	T17938	T17937	punct	", ",PTA
R5515	T17939	T17940	amod	persistent,arteriosus
R5516	T17940	T17937	appos	arteriosus,PTA
R5517	T17941	T17940	compound	truncus,arteriosus
R5518	T17942	T17898	punct	.,Ao
R552	T3036	T3033	conj	PTA,arch
R5521	T18219	T18220	amod	Abnormal,patterning
R5522	T18221	T18220	prep	of,patterning
R5523	T18222	T18223	det	the,PAAs
R5524	T18223	T18221	pobj	PAAs,of
R5525	T18224	T18220	prep	in,patterning
R5526	T18225	T18226	compound	Alk5,Cre
R5527	T18226	T18230	compound	Cre,mutants
R5528	T18227	T18226	punct	/,Cre
R5529	T18228	T18226	compound	Wnt1,Cre
R553	T3037	T3038	punct	[,9
R5530	T18229	T18226	punct	-,Cre
R5531	T18230	T18224	pobj	mutants,in
R5532	T18231	T18220	punct	.,patterning
R5533	T18233	T18234	amod	Left,view
R5534	T18235	T18234	amod	lateral,view
R5535	T18236	T18234	prep	after,view
R5536	T18237	T18238	amod	intracardiac,injections
R5537	T18238	T18236	pobj	injections,after
R5538	T18239	T18238	compound	ink,injections
R5539	T18240	T18241	aux	to,visualize
R554	T2921	T2916	cc	and,mispatterning
R5540	T18241	T18238	advcl	visualize,injections
R5541	T18242	T18243	det	the,PAAs
R5542	T18243	T18241	dobj	PAAs,visualize
R5543	T18244	T18243	amod	developing,PAAs
R5544	T18245	T18241	prep	at,visualize
R5545	T18246	T18245	pobj	E11.0,at
R5546	T18247	T18248	punct	(,B
R5547	T18248	T18246	parataxis	B,E11.0
R5548	T18249	T18248	nmod	A,B
R5549	T18250	T18248	punct	",",B
R555	T3038	T3013	parataxis	9,results
R5550	T18251	T18248	punct	),B
R5551	T18252	T18246	punct	", ",E11.0
R5552	T18253	T18246	conj	E12.0,E11.0
R5553	T18254	T18255	punct	(,D
R5554	T18255	T18253	parataxis	D,E12.0
R5555	T18256	T18255	nmod	C,D
R5556	T18257	T18255	punct	", ",D
R5557	T18258	T18255	punct	),D
R5558	T18259	T18253	cc	and,E12.0
R5559	T18260	T18253	conj	E13.0,E12.0
R556	T3039	T3038	nummod	8,9
R5560	T18261	T18262	punct	(,F
R5561	T18262	T18260	parataxis	F,E13.0
R5562	T18263	T18262	nmod	E,F
R5563	T18264	T18262	punct	", ",F
R5564	T18265	T18262	punct	),F
R5565	T18266	T18241	prep	in,visualize
R5566	T18267	T18266	pobj	controls,in
R5567	T18268	T18269	punct	(,E
R5568	T18269	T18267	parataxis	E,controls
R5569	T18270	T18269	nmod	A,E
R557	T3040	T3038	punct	",",9
R5570	T18271	T18269	punct	", ",E
R5571	T18272	T18269	nmod	C,E
R5572	T18273	T18269	punct	", ",E
R5573	T18274	T18269	punct	),E
R5574	T18275	T18267	cc	and,controls
R5575	T18276	T18277	compound	Alk5,Cre
R5576	T18277	T18281	compound	Cre,mutants
R5577	T18278	T18277	punct	/,Cre
R5578	T18279	T18277	compound	Wnt1,Cre
R5579	T18280	T18277	punct	-,Cre
R558	T2922	T2923	compound	outflow,tract
R5580	T18281	T18267	conj	mutants,controls
R5581	T18282	T18283	punct	(,F
R5582	T18283	T18281	parataxis	F,mutants
R5583	T18284	T18283	nmod	B,F
R5584	T18285	T18283	punct	", ",F
R5585	T18286	T18283	nmod	D,F
R5586	T18287	T18283	punct	", ",F
R5587	T18288	T18283	punct	),F
R5588	T18289	T18234	punct	.,view
R5589	T18291	T18292	nsubj	Arrow,points
R559	T3041	T3038	punct	],9
R5590	T18293	T18291	prep	in,Arrow
R5591	T18294	T18293	pobj	A,in
R5592	T18295	T18292	prep	to,points
R5593	T18296	T18297	det	the,duct
R5594	T18297	T18295	pobj	duct,to
R5595	T18298	T18297	amod	regressing,duct
R5596	T18299	T18297	amod	carotid,duct
R5597	T18300	T18292	punct	.,points
R5598	T18302	T18303	nsubj	Asterisk,depicts
R5599	T18304	T18302	prep	in,Asterisk
R56	T563	T564	amod	inappropriate,induction
R560	T3042	T2997	punct	.,display
R5600	T18305	T18304	pobj	B,in
R5601	T18306	T18307	det	the,structure
R5602	T18307	T18303	dobj	structure,depicts
R5603	T18308	T18307	amod	corresponding,structure
R5604	T18309	T18307	prep	in,structure
R5605	T18310	T18311	det	the,mutant
R5606	T18311	T18309	pobj	mutant,in
R5607	T18312	T18303	prep	with,depicts
R5608	T18313	T18314	det	no,signs
R5609	T18314	T18312	pobj	signs,with
R561	T2923	T2924	compound	tract,mal-alignments
R5610	T18315	T18314	prep	of,signs
R5611	T18316	T18315	pobj	regression,of
R5612	T18317	T18303	punct	.,depicts
R5613	T18319	T18320	nsubj	Asterisk,illustrates
R5614	T18321	T18319	prep	in,Asterisk
R5615	T18322	T18321	pobj	D,in
R5616	T18323	T18324	det	the,regression
R5617	T18324	T18320	dobj	regression,illustrates
R5618	T18325	T18324	amod	aberrant,regression
R5619	T18326	T18324	prep	of,regression
R562	T3044	T3045	nmod	BMPs,6
R5620	T18327	T18328	det	the,aorta
R5621	T18328	T18326	pobj	aorta,of
R5622	T18329	T18328	amod	dorsal,aorta
R5623	T18330	T18324	prep	between,regression
R5624	T18331	T18332	det	the,PAAs
R5625	T18332	T18330	pobj	PAAs,between
R5626	T18333	T18332	amod	4th,PAAs
R5627	T18334	T18333	cc	and,4th
R5628	T18335	T18333	conj	6th,4th
R5629	T18336	T18320	punct	.,illustrates
R563	T3045	T3046	nsubjpass	6,required
R5630	T18339	T18338	punct	", ",PT
R5631	T18340	T18341	amod	pulmonary,trunk
R5632	T18341	T18338	appos	trunk,PT
R5633	T18342	T18338	punct	;,PT
R5634	T18343	T18338	appos	Ao,PT
R5635	T18344	T18343	punct	", ",Ao
R5636	T18345	T18343	appos	Aorta,Ao
R5637	T18346	T18338	punct	;,PT
R5638	T18347	T18338	appos	TA,PT
R5639	T18348	T18347	punct	", ",TA
R564	T2924	T2916	conj	mal-alignments,mispatterning
R5640	T18349	T18350	compound	truncus,arteriosus
R5641	T18350	T18347	appos	arteriosus,TA
R5642	T18351	T18338	punct	.,PT
R5643	T18564	T18565	amod	Abnormal,Sac
R5644	T18566	T18565	amod	Aortic,Sac
R5645	T18567	T18565	prep	in,Sac
R5646	T18568	T18569	compound	Alk5,Cre
R5647	T18569	T18573	compound	Cre,mutants
R5648	T18570	T18569	punct	/,Cre
R5649	T18571	T18569	compound	Wnt1,Cre
R565	T2925	T2926	punct	[,5
R5650	T18572	T18569	punct	-,Cre
R5651	T18573	T18567	pobj	mutants,in
R5652	T18574	T18565	punct	.,Sac
R5653	T18576	T18577	compound	Alk5,Cre
R5654	T18577	T18581	compound	Cre,mutants
R5655	T18578	T18577	punct	/,Cre
R5656	T18579	T18577	compound	Wnt1,Cre
R5657	T18580	T18577	punct	-,Cre
R5658	T18581	T18582	nsubj	mutants,fail
R5659	T18583	T18584	punct	(,D
R566	T3047	T3045	cc	and,6
R5660	T18584	T18581	parataxis	D,mutants
R5661	T18585	T18584	nmod	B,D
R5662	T18586	T18584	punct	", ",D
R5663	T18587	T18584	punct	),D
R5664	T18588	T18589	aux	to,form
R5665	T18589	T18582	xcomp	form,fail
R5666	T18590	T18591	det	the,structure
R5667	T18591	T18589	dobj	structure,form
R5668	T18592	T18591	amod	typical,structure
R5669	T18593	T18594	npadvmod	T,shaped
R567	T2926	T2892	parataxis	5,led
R5670	T18594	T18591	amod	shaped,structure
R5671	T18595	T18594	punct	-,shaped
R5672	T18596	T18591	prep	of,structure
R5673	T18597	T18598	det	the,sac
R5674	T18598	T18596	pobj	sac,of
R5675	T18599	T18598	amod	aortic,sac
R5676	T18600	T18591	acl	seen,structure
R5677	T18601	T18600	prep	in,seen
R5678	T18602	T18601	pobj	controls,in
R5679	T18603	T18589	prep	at,form
R568	T2927	T2926	punct	],5
R5680	T18604	T18603	pobj	E11.5,at
R5681	T18605	T18582	punct	.,fail
R5682	T18607	T18608	punct	(,A
R5683	T18609	T18608	punct	", ",A
R5684	T18610	T18608	dep	C,A
R5685	T18611	T18608	punct	),A
R5686	T18612	T18608	punct	.,A
R5687	T18614	T18615	meta	A,image
R5688	T18616	T18614	punct	-,A
R5689	T18617	T18614	dep	B,A
R569	T2928	T2865	punct	.,demonstrated
R5690	T18618	T18615	punct	", ",image
R5691	T18619	T18615	amod	frontal,image
R5692	T18620	T18615	prep	of,image
R5693	T18621	T18622	npadvmod	ink,injected
R5694	T18622	T18624	amod	injected,embryos
R5695	T18623	T18622	punct	-,injected
R5696	T18624	T18620	pobj	embryos,of
R5697	T18625	T18615	punct	;,image
R5698	T18626	T18627	meta	C,sections
R5699	T18627	T18615	appos	sections,image
R57	T564	T562	pobj	induction,with
R570	T3048	T3049	punct	-,7
R5700	T18628	T18626	punct	-,C
R5701	T18629	T18626	dep	D,C
R5702	T18630	T18627	punct	", ",sections
R5703	T18631	T18627	amod	frontal,sections
R5704	T18632	T18633	punct	(,staining
R5705	T18633	T18627	parataxis	staining,sections
R5706	T18634	T18633	nmod	H,staining
R5707	T18635	T18634	cc	&,H
R5708	T18636	T18634	conj	E,H
R5709	T18637	T18633	punct	),staining
R571	T2930	T2931	advmod	Early,CNCCs
R5710	T18638	T18615	punct	.,image
R5711	T18640	T18641	nsubj	Arrows,point
R5712	T18641	T18646	ccomp	point,point
R5713	T18642	T18640	prep	in,Arrows
R5714	T18643	T18642	pobj	A,in
R5715	T18644	T18643	cc	and,A
R5716	T18645	T18643	conj	B,A
R5717	T18647	T18641	prep	to,point
R5718	T18648	T18649	det	the,level
R5719	T18649	T18647	pobj	level,to
R572	T3049	T3045	conj	7,6
R5720	T18650	T18649	prep	of,level
R5721	T18651	T18650	pobj	section,of
R5722	T18652	T18649	acl	shown,level
R5723	T18653	T18652	prep	in,shown
R5724	T18654	T18653	pobj	C,in
R5725	T18655	T18654	cc	and,C
R5726	T18656	T18654	conj	D,C
R5727	T18657	T18646	punct	(,point
R5728	T18658	T18659	amod	red,arrows
R5729	T18659	T18646	nsubj	arrows,point
R573	T3050	T3046	auxpass	are,required
R5730	T18660	T18659	prep	in,arrows
R5731	T18661	T18660	pobj	C,in
R5732	T18662	T18661	cc	and,C
R5733	T18663	T18661	conj	D,C
R5734	T18664	T18646	prep	to,point
R5735	T18665	T18666	det	the,sac
R5736	T18666	T18664	pobj	sac,to
R5737	T18667	T18666	amod	aortic,sac
R5738	T18668	T18666	prep	of,sac
R5739	T18669	T18670	det	the,control
R574	T3051	T3046	prep	for,required
R5740	T18670	T18668	pobj	control,of
R5741	T18671	T18670	cc	and,control
R5742	T18672	T18670	conj	mutant,control
R5743	T18673	T18646	punct	", ",point
R5744	T18674	T18646	advmod	respectively,point
R5745	T18675	T18646	punct	),point
R5746	T18676	T18646	punct	.,point
R575	T3052	T3053	amod	proper,formation
R5751	T18898	T18899	amod	Normal,migration
R5752	T18900	T18899	amod	cardiac,migration
R5753	T18901	T18899	compound	NCC,migration
R5754	T18902	T18899	prep	in,migration
R5755	T18903	T18904	compound	Alk5,Cre
R5756	T18904	T18908	compound	Cre,mutants
R5757	T18905	T18904	punct	/,Cre
R5758	T18906	T18904	compound	Wnt1,Cre
R5759	T18907	T18904	punct	-,Cre
R576	T3053	T3051	pobj	formation,for
R5760	T18908	T18902	pobj	mutants,in
R5761	T18909	T18899	punct	.,migration
R5762	T18911	T18912	det	The,OFT
R5763	T18912	T18913	nsubj	OFT,display
R5764	T18914	T18912	prep	of,OFT
R5765	T18915	T18914	pobj	controls,of
R5766	T18916	T18917	punct	(,E
R5767	T18917	T18915	parataxis	E,controls
R5768	T18918	T18917	nmod	A,E
R5769	T18919	T18917	punct	", ",E
R577	T3054	T3053	prep	of,formation
R5770	T18920	T18917	nmod	C,E
R5771	T18921	T18917	punct	", ",E
R5772	T18922	T18917	punct	),E
R5773	T18923	T18915	cc	and,controls
R5774	T18924	T18925	compound	Alk5,Cre
R5775	T18925	T18929	compound	Cre,mutants
R5776	T18926	T18925	punct	/,Cre
R5777	T18927	T18925	compound	Wnt1,Cre
R5778	T18928	T18925	punct	-,Cre
R5779	T18929	T18915	conj	mutants,controls
R578	T3055	T3056	det	the,cushions
R5780	T18930	T18931	punct	(,F
R5781	T18931	T18929	parataxis	F,mutants
R5782	T18932	T18931	nmod	B,F
R5783	T18933	T18931	punct	", ",F
R5784	T18934	T18931	nmod	D,F
R5785	T18935	T18931	punct	", ",F
R5786	T18936	T18931	punct	),F
R5787	T18937	T18938	amod	similar,patterns
R5788	T18938	T18913	dobj	patterns,display
R5789	T18939	T18938	compound	staining,patterns
R579	T2931	T2933	nsubjpass	CNCCs,shown
R5790	T18940	T18941	advmod	when,analyzed
R5791	T18941	T18913	advcl	analyzed,display
R5792	T18942	T18941	advcl	using,analyzed
R5793	T18943	T18944	det	the,assay
R5794	T18944	T18942	dobj	assay,using
R5795	T18945	T18944	compound	R26R,assay
R5796	T18946	T18947	compound	lineage,tracing
R5797	T18947	T18944	compound	tracing,assay
R5798	T18948	T18942	prep	at,using
R5799	T18949	T18948	pobj	E11.0,at
R58	T565	T564	punct	", ",induction
R580	T3056	T3054	pobj	cushions,of
R5800	T18950	T18913	punct	.,display
R5801	T18952	T18953	meta	A,staining
R5802	T18954	T18952	punct	-,A
R5803	T18955	T18952	dep	B,A
R5804	T18956	T18953	punct	", ",staining
R5805	T18957	T18958	amod	whole,mount
R5806	T18958	T18953	compound	mount,staining
R5807	T18959	T18960	punct	(,image
R5808	T18960	T18953	parataxis	image,staining
R5809	T18961	T18960	amod	left,image
R581	T3057	T3058	compound	outflow,tract
R5810	T18962	T18960	amod	lateral,image
R5811	T18963	T18960	punct	),image
R5812	T18964	T18953	punct	;,staining
R5813	T18965	T18966	meta	C,sections
R5814	T18966	T18953	appos	sections,staining
R5815	T18967	T18965	punct	-,C
R5816	T18968	T18965	dep	F,C
R5817	T18969	T18966	punct	", ",sections
R5818	T18970	T18966	amod	transverse,sections
R5819	T18971	T18966	prep	on,sections
R582	T2932	T2931	amod	migratory,CNCCs
R5820	T18972	T18973	det	the,level
R5821	T18973	T18971	pobj	level,on
R5822	T18974	T18973	prep	of,level
R5823	T18975	T18976	det	the,PAAs
R5824	T18976	T18974	pobj	PAAs,of
R5825	T18977	T18976	amod	4th,PAAs
R5826	T18978	T18979	punct	(,D
R5827	T18979	T18977	parataxis	D,4th
R5828	T18980	T18979	nmod	C,D
R5829	T18981	T18979	punct	", ",D
R583	T3058	T3056	compound	tract,cushions
R5830	T18982	T18979	punct	),D
R5831	T18983	T18977	cc	and,4th
R5832	T18984	T18977	conj	6th,4th
R5833	T18985	T18986	punct	(,F
R5834	T18986	T18984	parataxis	F,6th
R5835	T18987	T18986	nmod	E,F
R5836	T18988	T18986	punct	", ",F
R5837	T18989	T18986	punct	),F
R5838	T18990	T18953	punct	.,staining
R5839	T18992	T18993	nsubj	Arrows,point
R584	T3059	T3060	punct	[,10
R5840	T18994	T18995	punct	(,A
R5841	T18995	T18992	parataxis	A,Arrows
R5842	T18996	T18997	punct	-,F
R5843	T18997	T18995	prep	F,A
R5844	T18998	T18995	punct	),A
R5845	T18999	T18993	prep	to,point
R5846	T19000	T19001	det	the,location
R5847	T19001	T18999	pobj	location,to
R5848	T19002	T19003	advmod	most,proximal
R5849	T19003	T19001	amod	proximal,location
R585	T3060	T3053	parataxis	10,formation
R5850	T19004	T19001	acl	staining,location
R5851	T19005	T19004	advcl	positive,staining
R5852	T19006	T19005	prep	for,positive
R5853	T19007	T19008	det	the,activity
R5854	T19008	T19006	pobj	activity,for
R5855	T19009	T19010	compound	β,galactosidase
R5856	T19010	T19008	compound	galactosidase,activity
R5857	T19011	T19010	punct	-,galactosidase
R5858	T19012	T18993	punct	.,point
R5859	T19235	T19236	det	The,OFT
R586	T2934	T2933	aux	have,shown
R5860	T19236	T19238	nsubj	OFT,fails
R5861	T19237	T19236	amod	truncal,OFT
R5862	T19239	T19240	aux	to,rotate
R5863	T19240	T19238	xcomp	rotate,fails
R5864	T19241	T19240	prep	in,rotate
R5865	T19242	T19243	compound	Alk5,Cre
R5866	T19243	T19247	compound	Cre,mutants
R5867	T19244	T19243	punct	/,Cre
R5868	T19245	T19243	compound	Wnt1,Cre
R5869	T19246	T19243	punct	-,Cre
R587	T3061	T3060	punct	],10
R5870	T19247	T19241	pobj	mutants,in
R5871	T19248	T19238	punct	.,fails
R5872	T19250	T19251	amod	Left,images
R5873	T19251	T19265	nsubj	images,demonstrate
R5874	T19252	T19253	punct	(,C
R5875	T19253	T19250	parataxis	C,Left
R5876	T19254	T19253	nmod	A,C
R5877	T19255	T19253	punct	", ",C
R5878	T19256	T19253	punct	),C
R5879	T19257	T19250	cc	and,Left
R588	T3062	T3046	punct	", ",required
R5880	T19258	T19250	conj	right,Left
R5881	T19259	T19260	punct	(,D
R5882	T19260	T19258	parataxis	D,right
R5883	T19261	T19260	nmod	B,D
R5884	T19262	T19260	punct	", ",D
R5885	T19263	T19260	punct	),D
R5886	T19264	T19251	amod	lateral,images
R5887	T19266	T19251	prep	of,images
R5888	T19267	T19268	npadvmod	ink,injected
R5889	T19268	T19270	amod	injected,embryos
R589	T3063	T3064	mark	while,needed
R5890	T19269	T19268	punct	-,injected
R5891	T19270	T19266	pobj	embryos,of
R5892	T19271	T19270	nmod	control,embryos
R5893	T19272	T19273	punct	(,A
R5894	T19273	T19271	parataxis	A,control
R5895	T19274	T19275	punct	-,B
R5896	T19275	T19273	prep	B,A
R5897	T19276	T19273	punct	),A
R5898	T19277	T19271	cc	and,control
R5899	T19278	T19271	conj	mutant,control
R59	T566	T564	conj	migration,induction
R590	T2935	T2933	auxpass	been,shown
R5900	T19279	T19280	punct	(,C
R5901	T19280	T19278	parataxis	C,mutant
R5902	T19281	T19282	punct	-,D
R5903	T19282	T19280	prep	D,C
R5904	T19283	T19280	punct	),C
R5905	T19284	T19251	prep	at,images
R5906	T19285	T19284	pobj	E11.5,at
R5907	T19286	T19287	det	the,OFT
R5908	T19287	T19265	dobj	OFT,demonstrate
R5909	T19288	T19289	advmod	abnormally,straight
R591	T3064	T3046	advcl	needed,required
R5910	T19289	T19287	amod	straight,OFT
R5911	T19290	T19265	prep	in,demonstrate
R5912	T19291	T19290	pobj	mutants,in
R5913	T19292	T19291	acl	lacking,mutants
R5914	T19293	T19294	det	the,transition
R5915	T19294	T19292	dobj	transition,lacking
R5916	T19295	T19294	amod	typical,transition
R5917	T19296	T19294	amod	conotruncal,transition
R5918	T19297	T19298	punct	(,arrow
R5919	T19298	T19294	parataxis	arrow,transition
R592	T3065	T3066	nmod	BMP,receptor
R5920	T19299	T19298	amod	black,arrow
R5921	T19300	T19298	prep	in,arrow
R5922	T19301	T19300	pobj	A,in
R5923	T19302	T19298	cc	vs.,arrow
R5924	T19303	T19304	amod	black,arrowhead
R5925	T19304	T19298	conj	arrowhead,arrow
R5926	T19305	T19304	prep	in,arrowhead
R5927	T19306	T19305	pobj	C,in
R5928	T19307	T19298	punct	),arrow
R5929	T19308	T19294	acl	seen,transition
R593	T3066	T3064	nsubjpass	receptor,needed
R5930	T19309	T19308	prep	in,seen
R5931	T19310	T19309	pobj	control,in
R5932	T19311	T19265	punct	.,demonstrate
R5933	T19313	T19314	amod	Red,arrowhead
R5934	T19314	T19315	nsubj	arrowhead,points
R5935	T19316	T19317	punct	(,C
R5936	T19317	T19314	parataxis	C,arrowhead
R5937	T19318	T19317	punct	),C
R5938	T19319	T19315	prep	to,points
R5939	T19320	T19321	det	the,sac
R594	T2936	T2937	aux	to,retain
R5940	T19321	T19319	pobj	sac,to
R5941	T19322	T19323	advmod	abnormally,shaped
R5942	T19323	T19321	amod	shaped,sac
R5943	T19324	T19321	amod	aortic,sac
R5944	T19325	T19315	punct	.,points
R5945	T19327	T19328	amod	Red,s
R5946	T19329	T19328	punct	"""",s
R5947	T19330	T19328	punct	"""",s
R5948	T19331	T19328	punct	", ",s
R5949	T19332	T19333	amod	aortic,sac
R595	T3067	T3066	nmod	type,receptor
R5950	T19333	T19328	appos	sac,s
R5951	T19334	T19328	punct	;,s
R5952	T19335	T19328	appos	t,s
R5953	T19336	T19335	punct	", ",t
R5954	T19337	T19335	appos	truncus,t
R5955	T19338	T19328	punct	;,s
R5956	T19339	T19328	appos	c,s
R5957	T19340	T19339	punct	", ",c
R5958	T19341	T19339	appos	conus,c
R5959	T19342	T19328	punct	.,s
R596	T3068	T3067	nummod	II,type
R5960	T19663	T19664	nsubj	Signaling,controls
R5961	T19665	T19663	prep	via,Signaling
R5962	T19666	T19665	pobj	ALK5,via
R5963	T19667	T19666	cc	and,ALK5
R5964	T19668	T19666	conj	ALK2,ALK5
R5965	T19669	T19670	amod	different,aspects
R5966	T19670	T19664	dobj	aspects,controls
R5967	T19671	T19670	prep	of,aspects
R5968	T19672	T19673	amod	aortico,pulmonary
R5969	T19673	T19675	amod	pulmonary,septation
R597	T3069	T3064	auxpass	is,needed
R5970	T19674	T19673	punct	-,pulmonary
R5971	T19675	T19671	pobj	septation,of
R5972	T19676	T19664	punct	.,controls
R5973	T19678	T19679	amod	Frontal,sections
R5974	T19680	T19679	prep	from,sections
R5975	T19681	T19680	amod	distal,from
R5976	T19682	T19683	punct	(,row
R5977	T19683	T19681	parataxis	row,distal
R5978	T19684	T19683	amod	top,row
R5979	T19685	T19683	punct	),row
R598	T2937	T2933	xcomp	retain,shown
R5980	T19686	T19680	prep	to,from
R5981	T19687	T19686	amod	proximal,to
R5982	T19688	T19689	punct	(,row
R5983	T19689	T19687	parataxis	row,proximal
R5984	T19690	T19689	amod	bottom,row
R5985	T19691	T19689	punct	),row
R5986	T19692	T19679	prep	of,sections
R5987	T19693	T19694	det	the,samples
R5988	T19694	T19692	pobj	samples,of
R5989	T19695	T19694	nmod	control,samples
R599	T3070	T3064	prep	for,needed
R5990	T19696	T19697	punct	(,A
R5991	T19697	T19695	parataxis	A,control
R5992	T19698	T19699	punct	-,F
R5993	T19699	T19697	prep	F,A
R5994	T19700	T19697	punct	),A
R5995	T19701	T19695	punct	", ",control
R5996	T19702	T19703	compound	Alk5,Cre
R5997	T19703	T19707	compound	Cre,mutant
R5998	T19704	T19703	punct	/,Cre
R5999	T19705	T19703	compound	Wnt1,Cre
R60	T567	T566	punct	", ",migration
R600	T3071	T3072	amod	proper,development
R6000	T19706	T19703	punct	-,Cre
R6001	T19707	T19695	conj	mutant,control
R6002	T19708	T19709	punct	(,G
R6003	T19709	T19707	parataxis	G,mutant
R6004	T19710	T19711	punct	-,L
R6005	T19711	T19709	prep	L,G
R6006	T19712	T19709	punct	),G
R6007	T19713	T19707	cc	and,mutant
R6008	T19714	T19715	compound	Alk2,Cre
R6009	T19715	T19719	compound	Cre,mutant
R601	T2938	T2939	det	a,degree
R6010	T19716	T19715	punct	/,Cre
R6011	T19717	T19715	compound	Wnt1,Cre
R6012	T19718	T19715	punct	-,Cre
R6013	T19719	T19707	conj	mutant,mutant
R6014	T19720	T19721	punct	(,M
R6015	T19721	T19719	parataxis	M,mutant
R6016	T19722	T19723	punct	-,R
R6017	T19723	T19721	prep	R,M
R6018	T19724	T19721	punct	),M
R6019	T19725	T19726	punct	(,E11.5
R602	T3072	T3070	pobj	development,for
R6020	T19726	T19694	parataxis	E11.5,samples
R6021	T19727	T19726	punct	),E11.5
R6022	T19728	T19679	punct	.,sections
R6023	T19730	T19731	meta	A,staining
R6024	T19732	T19730	punct	", ",A
R6025	T19733	T19730	dep	C,A
R6026	T19734	T19730	punct	", ",A
R6027	T19735	T19730	dep	E,A
R6028	T19736	T19730	punct	", ",A
R6029	T19737	T19730	dep	G,A
R603	T3073	T3072	prep	of,development
R6030	T19738	T19730	punct	", ",A
R6031	T19739	T19730	dep	I,A
R6032	T19740	T19730	punct	", ",A
R6033	T19741	T19730	dep	K,A
R6034	T19742	T19730	punct	", ",A
R6035	T19743	T19730	dep	M,A
R6036	T19744	T19730	punct	", ",A
R6037	T19745	T19730	dep	O,A
R6038	T19746	T19730	punct	", ",A
R6039	T19747	T19730	dep	Q,A
R604	T3074	T3075	det	the,ridges
R6040	T19748	T19731	punct	", ",staining
R6041	T19749	T19731	nmod	H,staining
R6042	T19750	T19749	cc	&,H
R6043	T19751	T19749	conj	E,H
R6044	T19752	T19731	punct	;,staining
R6045	T19753	T19754	meta	B,staining
R6046	T19754	T19731	appos	staining,staining
R6047	T19755	T19753	punct	", ",B
R6048	T19756	T19753	dep	D,B
R6049	T19757	T19753	punct	", ",B
R605	T2939	T2937	dobj	degree,retain
R6050	T19758	T19753	dep	F,B
R6051	T19759	T19753	punct	", ",B
R6052	T19760	T19753	dep	H,B
R6053	T19761	T19753	punct	", ",B
R6054	T19762	T19753	dep	J,B
R6055	T19763	T19753	punct	", ",B
R6056	T19764	T19753	dep	L,B
R6057	T19765	T19753	punct	", ",B
R6058	T19766	T19753	dep	N,B
R6059	T19767	T19753	punct	", ",B
R606	T3075	T3073	pobj	ridges,of
R6060	T19768	T19753	dep	P,B
R6061	T19769	T19753	punct	", ",B
R6062	T19770	T19753	dep	R,B
R6063	T19771	T19754	punct	", ",staining
R6064	T19772	T19754	amod	double,staining
R6065	T19773	T19754	prep	for,staining
R6066	T19774	T19773	pobj	αSMA,for
R6067	T19775	T19776	punct	(,brown
R6068	T19776	T19774	parataxis	brown,αSMA
R6069	T19777	T19776	punct	),brown
R607	T3076	T3075	amod	conotruncal,ridges
R6070	T19778	T19774	cc	and,αSMA
R6071	T19779	T19780	compound	β,galactosidase
R6072	T19780	T19774	conj	galactosidase,αSMA
R6073	T19781	T19780	punct	-,galactosidase
R6074	T19782	T19783	punct	(,assay
R6075	T19783	T19780	parataxis	assay,galactosidase
R6076	T19784	T19783	amod	green,assay
R6077	T19785	T19783	punct	;,assay
R6078	T19786	T19783	compound	R26R,assay
R6079	T19787	T19783	compound	reporter,assay
R608	T3077	T3078	punct	[,11
R6080	T19788	T19783	punct	),assay
R6081	T19789	T19731	punct	.,staining
R6082	T19791	T19792	dep	6,depict
R6083	T19793	T19791	punct	", ",6
R6084	T19794	T19795	det	the,PAA
R6085	T19795	T19791	appos	PAA,6
R6086	T19796	T19795	amod	6th,PAA
R6087	T19797	T19791	punct	;,6
R6088	T19798	T19791	appos	AS,6
R6089	T19799	T19798	punct	", ",AS
R609	T2940	T2939	amod	considerable,degree
R6090	T19800	T19801	amod	aortic,sac
R6091	T19801	T19798	appos	sac,AS
R6092	T19802	T19791	punct	;,6
R6093	T19803	T19791	appos	TA,6
R6094	T19804	T19803	punct	", ",TA
R6095	T19805	T19806	compound	truncus,arteriosus
R6096	T19806	T19803	appos	arteriosus,TA
R6097	T19807	T19791	punct	;,6
R6098	T19808	T19791	appos	Ao,6
R6099	T19809	T19808	punct	", ",Ao
R61	T568	T566	conj	differentiation,migration
R610	T3078	T3046	parataxis	11,required
R6100	T19810	T19808	appos	Aorta,Ao
R6101	T19811	T19791	punct	;,6
R6102	T19812	T19791	appos	PT,6
R6103	T19813	T19812	punct	", ",PT
R6104	T19814	T19815	amod	pulmonary,trunk
R6105	T19815	T19812	appos	trunk,PT
R6106	T19816	T19792	punct	;,depict
R6107	T19817	T19792	nsubj	Asterisks,depict
R6108	T19818	T19817	prep	in,Asterisks
R6109	T19819	T19818	pobj	A,in
R611	T3079	T3078	punct	],11
R6110	T19820	T19819	punct	", ",A
R6111	T19821	T19819	conj	B,A
R6112	T19822	T19821	punct	", ",B
R6113	T19823	T19821	conj	M,B
R6114	T19824	T19823	cc	and,M
R6115	T19825	T19823	conj	N,M
R6116	T19826	T19827	det	the,mesenchyme
R6117	T19827	T19792	dobj	mesenchyme,depict
R6118	T19828	T19827	nmod	AP,mesenchyme
R6119	T19829	T19827	amod	septal,mesenchyme
R612	T2941	T2939	prep	of,degree
R6120	T19830	T19792	punct	.,depict
R6122	T20179	T20180	amod	Aberrant,apoptosis
R6123	T20181	T20180	prep	of,apoptosis
R6124	T20182	T20181	pobj	NCCs,of
R6125	T20183	T20180	prep	in,apoptosis
R6126	T20184	T20185	compound	Alk5,Cre
R6127	T20185	T20189	compound	Cre,mutants
R6128	T20186	T20185	punct	/,Cre
R6129	T20187	T20185	compound	Wnt1,Cre
R613	T3080	T3046	punct	.,required
R6130	T20188	T20185	punct	-,Cre
R6131	T20189	T20183	pobj	mutants,in
R6132	T20190	T20180	punct	.,apoptosis
R6133	T20192	T20193	nmod	TUNEL,staining
R6134	T20193	T20209	nsubj	staining,demonstrates
R6135	T20194	T20195	punct	(,A
R6136	T20195	T20192	parataxis	A,TUNEL
R6137	T20196	T20197	punct	-,H
R6138	T20197	T20195	prep	H,A
R6139	T20198	T20195	punct	),A
R614	T3082	T3083	advmod	Moreover,shown
R6140	T20199	T20192	cc	and,TUNEL
R6141	T20200	T20201	amod	Cleaved,Caspase
R6142	T20201	T20192	conj	Caspase,TUNEL
R6143	T20202	T20201	punct	-,Caspase
R6144	T20203	T20201	nummod	3,Caspase
R6145	T20204	T20205	punct	(,J
R6146	T20205	T20201	parataxis	J,Caspase
R6147	T20206	T20205	nmod	I,J
R6148	T20207	T20205	punct	", ",J
R6149	T20208	T20205	punct	),J
R615	T2942	T2941	pobj	plasticity,of
R6150	T20210	T20193	prep	at,staining
R6151	T20211	T20210	pobj	E11.0,at
R6152	T20212	T20213	det	a,increase
R6153	T20213	T20209	dobj	increase,demonstrates
R6154	T20214	T20213	amod	notable,increase
R6155	T20215	T20213	prep	in,increase
R6156	T20216	T20215	pobj	apoptosis,in
R6157	T20217	T20213	prep	in,increase
R6158	T20218	T20219	compound	Alk5,Cre
R6159	T20219	T20223	compound	Cre,mutants
R616	T3084	T3083	punct	", ",shown
R6160	T20220	T20219	punct	/,Cre
R6161	T20221	T20219	compound	Wnt1,Cre
R6162	T20222	T20219	punct	-,Cre
R6163	T20223	T20217	pobj	mutants,in
R6164	T20224	T20225	punct	(,J
R6165	T20225	T20223	parataxis	J,mutants
R6166	T20226	T20225	nmod	B,J
R6167	T20227	T20225	punct	", ",J
R6168	T20228	T20225	nmod	H,J
R6169	T20229	T20225	punct	", ",J
R617	T3153	T3152	pobj	phosphorylation,to
R6170	T20230	T20225	punct	),J
R6171	T20231	T20213	prep	on,increase
R6172	T20232	T20233	det	the,level
R6173	T20233	T20231	pobj	level,on
R6174	T20234	T20233	amod	aortic,level
R6175	T20235	T20233	compound	sac,level
R6176	T20236	T20237	advmod	when,compared
R6177	T20237	T20213	advcl	compared,increase
R6178	T20238	T20237	prep	to,compared
R6179	T20239	T20238	pobj	controls,to
R618	T3085	T3086	amod	neural,crest
R6180	T20240	T20241	punct	(,I
R6181	T20241	T20239	parataxis	I,controls
R6182	T20242	T20241	nmod	A,I
R6183	T20243	T20241	punct	", ",I
R6184	T20244	T20241	nmod	G,I
R6185	T20245	T20241	punct	", ",I
R6186	T20246	T20241	punct	),I
R6187	T20247	T20239	cc	or,controls
R6188	T20248	T20249	compound	Alk2,Cre
R6189	T20249	T20253	compound	Cre,mutants
R619	T3086	T3087	npadvmod	crest,specific
R6190	T20250	T20249	punct	/,Cre
R6191	T20251	T20249	compound	Wnt1,Cre
R6192	T20252	T20249	punct	-,Cre
R6193	T20253	T20239	conj	mutants,controls
R6194	T20254	T20255	punct	(,C
R6195	T20255	T20253	parataxis	C,mutants
R6196	T20256	T20255	punct	),C
R6197	T20257	T20258	punct	(,sections
R6198	T20258	T20253	parataxis	sections,mutants
R6199	T20259	T20258	amod	frontal,sections
R62	T569	T568	cc	and,differentiation
R620	T3087	T3089	amod	specific,deletion
R6200	T20260	T20258	punct	),sections
R6201	T20261	T20209	punct	", ",demonstrates
R6202	T20262	T20263	mark	while,demonstrate
R6203	T20263	T20209	advcl	demonstrate,demonstrates
R6204	T20264	T20263	nsubj	sections,demonstrate
R6205	T20265	T20264	prep	on,sections
R6206	T20266	T20267	det	the,level
R6207	T20267	T20265	pobj	level,on
R6208	T20268	T20267	compound	OFT,level
R6209	T20269	T20263	aux	do,demonstrate
R621	T3088	T3087	punct	-,specific
R6210	T20270	T20263	neg	not,demonstrate
R6211	T20271	T20263	dobj	differences,demonstrate
R6212	T20272	T20271	prep	between,differences
R6213	T20273	T20272	pobj	controls,between
R6214	T20274	T20275	punct	(,D
R6215	T20275	T20273	parataxis	D,controls
R6216	T20276	T20275	punct	),D
R6217	T20277	T20273	cc	and,controls
R6218	T20278	T20279	nmod	Alk5,mutants
R6219	T20279	T20273	conj	mutants,controls
R622	T3154	T3153	cc	and,phosphorylation
R6220	T20280	T20281	punct	(,E
R6221	T20281	T20278	parataxis	E,Alk5
R6222	T20282	T20281	punct	),E
R6223	T20283	T20278	cc	or,Alk5
R6224	T20284	T20278	conj	Alk2,Alk5
R6225	T20285	T20286	punct	(,F
R6226	T20286	T20284	parataxis	F,Alk2
R6227	T20287	T20286	punct	),F
R6228	T20288	T20209	punct	.,demonstrates
R6229	T20290	T20291	meta	G,demonstrates
R623	T3089	T3083	nsubjpass	deletion,shown
R6230	T20292	T20290	punct	",",G
R6231	T20293	T20290	dep	H,G
R6232	T20294	T20291	punct	", ",demonstrates
R6233	T20295	T20296	compound	TUNEL,staining
R6234	T20296	T20291	nsubj	staining,demonstrates
R6235	T20297	T20296	prep	of,staining
R6236	T20298	T20299	npadvmod	lacZ,stained
R6237	T20299	T20301	amod	stained,embryos
R6238	T20300	T20299	punct	-,stained
R6239	T20301	T20297	pobj	embryos,of
R624	T3090	T3089	prep	of,deletion
R6240	T20302	T20303	mark	that,are
R6241	T20303	T20291	ccomp	are,demonstrates
R6242	T20304	T20305	amod	apoptotic,cells
R6243	T20305	T20303	nsubj	cells,are
R6244	T20306	T20303	prep	of,are
R6245	T20307	T20308	amod	neural,crest
R6246	T20308	T20309	compound	crest,origin
R6247	T20309	T20306	pobj	origin,of
R6248	T20310	T20291	punct	.,demonstrates
R6249	T20313	T20312	punct	", ",G
R625	T3091	T3092	det	the,Alk2
R6250	T20314	T20312	appos	control,G
R6251	T20315	T20312	punct	;,G
R6252	T20316	T20312	appos	H,G
R6253	T20317	T20316	punct	", ",H
R6254	T20318	T20316	appos	mutant,H
R6255	T20319	T20312	punct	.,G
R6256	T20321	T20322	dep	AS,point
R6257	T20323	T20321	punct	", ",AS
R6258	T20324	T20325	amod	aortic,sac
R6259	T20325	T20321	appos	sac,AS
R626	T3092	T3090	pobj	Alk2,of
R6260	T20326	T20322	punct	", ",point
R6261	T20327	T20322	nsubj	arrows,point
R6262	T20328	T20322	prep	to,point
R6263	T20329	T20328	pobj	clusters,to
R6264	T20330	T20329	prep	of,clusters
R6265	T20331	T20332	amod	apoptotic,cells
R6266	T20332	T20330	pobj	cells,of
R6267	T20333	T20329	acl	surrounding,clusters
R6268	T20334	T20335	det	the,sac
R6269	T20335	T20333	dobj	sac,surrounding
R627	T3093	T3092	nmod	BMP,Alk2
R6270	T20336	T20335	amod	aortic,sac
R6271	T20337	T20322	punct	.,point
R6272	T20789	T20790	amod	Pharyngeal,organs
R6273	T20790	T20791	nsubj	organs,fail
R6274	T20792	T20793	aux	to,migrate
R6275	T20793	T20791	xcomp	migrate,fail
R6276	T20794	T20793	prep	in,migrate
R6277	T20795	T20796	compound	Alk5,Cre
R6278	T20796	T20800	compound	Cre,mutants
R6279	T20797	T20796	punct	/,Cre
R628	T3094	T3092	nmod	type,Alk2
R6280	T20798	T20796	compound	Wnt1,Cre
R6281	T20799	T20796	punct	-,Cre
R6282	T20800	T20794	pobj	mutants,in
R6283	T20801	T20791	punct	.,fail
R6284	T20803	T20804	prep	At,was
R6285	T20805	T20803	pobj	E14.0,At
R6286	T20806	T20804	punct	", ",was
R6287	T20807	T20808	det	the,thymus
R6288	T20808	T20804	nsubj	thymus,was
R6289	T20809	T20804	neg	not,was
R629	T3155	T3153	conj	activation,phosphorylation
R6290	T20810	T20804	acomp	detectable,was
R6291	T20811	T20804	prep	in,was
R6292	T20812	T20813	det	the,mediastinum
R6293	T20813	T20811	pobj	mediastinum,in
R6294	T20814	T20813	amod	superior,mediastinum
R6295	T20815	T20816	punct	(,asterisks
R6296	T20816	T20813	parataxis	asterisks,mediastinum
R6297	T20817	T20816	punct	),asterisks
R6298	T20818	T20804	prep	in,was
R6299	T20819	T20820	compound	Alk5,mutants
R63	T570	T568	conj	patterning,differentiation
R630	T3095	T3094	nummod	I,type
R6300	T20820	T20818	pobj	mutants,in
R6301	T20821	T20822	punct	(,D
R6302	T20822	T20820	parataxis	D,mutants
R6303	T20823	T20822	punct	),D
R6304	T20824	T20804	punct	", ",was
R6305	T20825	T20826	advmod	when,compared
R6306	T20826	T20804	advcl	compared,was
R6307	T20827	T20826	prep	to,compared
R6308	T20828	T20827	pobj	controls,to
R6309	T20829	T20830	punct	(,A
R631	T3096	T3092	compound	receptors,Alk2
R6310	T20830	T20826	parataxis	A,compared
R6311	T20831	T20830	punct	),A
R6312	T20832	T20804	punct	.,was
R6313	T20834	T20835	amod	Serial,sectioning
R6314	T20835	T20836	nsubj	sectioning,revealed
R6315	T20837	T20838	mark	that,failed
R6316	T20838	T20836	ccomp	failed,revealed
R6317	T20839	T20840	det	the,primordia
R6318	T20840	T20838	nsubj	primordia,failed
R6319	T20841	T20840	amod	thymic,primordia
R632	T3097	T3092	cc	and,Alk2
R6320	T20842	T20838	aux	had,failed
R6321	T20843	T20844	aux	to,descend
R6322	T20844	T20838	xcomp	descend,failed
R6323	T20845	T20838	cc	and,failed
R6324	T20846	T20847	auxpass	were,seen
R6325	T20847	T20838	conj	seen,failed
R6326	T20848	T20847	advmod	bilaterally,seen
R6327	T20849	T20847	prep	in,seen
R6328	T20850	T20851	det	the,region
R6329	T20851	T20849	pobj	region,in
R633	T3156	T3153	prep	of,phosphorylation
R6330	T20852	T20853	amod	upper,pharyngeal
R6331	T20853	T20851	amod	pharyngeal,region
R6332	T20854	T20855	punct	(,F
R6333	T20855	T20851	parataxis	F,region
R6334	T20856	T20855	nmod	E,F
R6335	T20857	T20855	punct	", ",F
R6336	T20858	T20855	punct	),F
R6337	T20859	T20851	acl	surrounded,region
R6338	T20860	T20859	agent	by,surrounded
R6339	T20861	T20862	amod	neural,crest
R634	T3098	T3092	conj	Alk3,Alk2
R6340	T20862	T20863	npadvmod	crest,derived
R6341	T20863	T20864	amod	derived,mesenchyme
R6342	T20864	T20860	pobj	mesenchyme,by
R6343	T20865	T20866	punct	(,cells
R6344	T20866	T20859	parataxis	cells,surrounded
R6345	T20867	T20866	amod	blue,cells
R6346	T20868	T20866	compound	staining,cells
R6347	T20869	T20866	prep	in,cells
R6348	T20870	T20869	pobj	E,in
R6349	T20871	T20866	punct	),cells
R635	T3099	T3083	aux	has,shown
R6350	T20872	T20836	punct	.,revealed
R6351	T20874	T20875	prep	In,associated
R6352	T20876	T20874	pobj	controls,In
R6353	T20877	T20875	punct	", ",associated
R6354	T20878	T20879	det	the,glands
R6355	T20879	T20875	nsubjpass	glands,associated
R6356	T20880	T20879	amod	parathyroid,glands
R6357	T20881	T20875	auxpass	were,associated
R6358	T20882	T20875	advmod	properly,associated
R6359	T20883	T20875	prep	with,associated
R636	T3100	T3083	auxpass	been,shown
R6360	T20884	T20885	det	the,glands
R6361	T20885	T20883	pobj	glands,with
R6362	T20886	T20885	amod	thyroid,glands
R6363	T20887	T20888	punct	(,arrows
R6364	T20888	T20875	parataxis	arrows,associated
R6365	T20889	T20888	prep	in,arrows
R6366	T20890	T20889	pobj	C,in
R6367	T20891	T20888	punct	),arrows
R6368	T20892	T20875	punct	", ",associated
R6369	T20893	T20894	mark	while,associated
R637	T3101	T3102	aux	to,lead
R6370	T20894	T20875	advcl	associated,associated
R6371	T20895	T20894	prep	in,associated
R6372	T20896	T20897	compound	Alk5,mutants
R6373	T20897	T20895	pobj	mutants,in
R6374	T20898	T20899	det	the,glands
R6375	T20899	T20894	nsubjpass	glands,associated
R6376	T20900	T20899	amod	parathyroid,glands
R6377	T20901	T20894	auxpass	were,associated
R6378	T20902	T20894	prep	with,associated
R6379	T20903	T20904	det	the,primordia
R638	T3157	T3158	nmod	type,receptors
R6380	T20904	T20902	pobj	primordia,with
R6381	T20905	T20904	amod	thymic,primordia
R6382	T20906	T20907	punct	(,arrows
R6383	T20907	T20894	parataxis	arrows,associated
R6384	T20908	T20907	prep	in,arrows
R6385	T20909	T20908	pobj	F,in
R6386	T20910	T20907	punct	),arrows
R6387	T20911	T20875	punct	.,associated
R6388	T20913	T20914	advmod	However,expressed
R6389	T20915	T20914	punct	", ",expressed
R639	T3102	T3083	xcomp	lead,shown
R6390	T20916	T20917	det	both,controls
R6391	T20917	T20914	nsubj	controls,expressed
R6392	T20918	T20917	cc	and,controls
R6393	T20919	T20917	conj	mutants,controls
R6394	T20920	T20921	compound	parathyroid,hormone
R6395	T20921	T20914	dobj	hormone,expressed
R6396	T20922	T20921	punct	(,hormone
R6397	T20923	T20921	appos	PTH,hormone
R6398	T20924	T20914	punct	),expressed
R6399	T20925	T20914	prep	at,expressed
R64	T571	T564	prep	of,induction
R640	T3103	T3102	prep	to,lead
R6400	T20926	T20927	amod	comparable,levels
R6401	T20927	T20925	pobj	levels,at
R6402	T20928	T20929	punct	(,staining
R6403	T20929	T20914	parataxis	staining,expressed
R6404	T20930	T20929	amod	blue,staining
R6405	T20931	T20929	prep	in,staining
R6406	T20932	T20931	pobj	C,in
R6407	T20933	T20932	cc	and,C
R6408	T20934	T20932	conj	F,C
R6409	T20935	T20929	punct	),staining
R641	T3158	T3156	pobj	receptors,of
R6410	T20936	T20914	punct	.,expressed
R6411	T20938	T20939	meta	A,staining
R6412	T20940	T20938	cc	and,A
R6413	T20941	T20938	conj	D,A
R6414	T20942	T20939	punct	", ",staining
R6415	T20943	T20939	nmod	hematoxyllin,staining
R6416	T20944	T20943	cc	and,hematoxyllin
R6417	T20945	T20943	conj	eosin,hematoxyllin
R6418	T20946	T20939	punct	;,staining
R6419	T20947	T20948	meta	B,R
R642	T3104	T3105	amod	defective,septation
R6420	T20948	T20939	appos	R,staining
R6421	T20949	T20947	cc	and,B
R6422	T20950	T20947	conj	E,B
R6423	T20951	T20948	punct	", ",R
R6424	T20952	T20948	compound	R26,R
R6425	T20953	T20954	compound	lineage,assay
R6426	T20954	T20948	appos	assay,R
R6427	T20955	T20954	compound	tracing,assay
R6428	T20956	T20954	punct	–,assay
R6429	T20957	T20954	amod	counterstaining,assay
R643	T3105	T3103	pobj	septation,to
R6430	T20958	T20948	prep	with,R
R6431	T20959	T20958	pobj	eosin,with
R6432	T20960	T20939	punct	;,staining
R6433	T20961	T20962	meta	C,hybridization
R6434	T20962	T20939	appos	hybridization,staining
R6435	T20963	T20961	cc	and,C
R6436	T20964	T20961	conj	F,C
R6437	T20965	T20962	punct	", ",hybridization
R6438	T20966	T20962	nmod	section,hybridization
R6439	T20967	T20968	nmod	in,situ
R644	T3106	T3107	amod	aortico,pulmonary
R6440	T20968	T20962	nmod	situ,hybridization
R6441	T20969	T20962	prep	for,hybridization
R6442	T20970	T20969	pobj	PTH,for
R6443	T20971	T20970	punct	–,PTH
R6444	T20972	T20970	amod	counterstaining,PTH
R6445	T20973	T20970	prep	with,PTH
R6446	T20974	T20973	pobj	eosin,with
R6447	T20975	T20939	punct	.,staining
R6448	T20977	T20978	dep	T,depict
R6449	T20979	T20977	punct	", ",T
R645	T3159	T3157	nummod	I,type
R6450	T20980	T20977	appos	thymus,T
R6451	T20981	T20978	punct	;,depict
R6452	T20982	T20978	dep	Th,depict
R6453	T20983	T20982	punct	", ",Th
R6454	T20984	T20982	appos	thyroid,Th
R6455	T20985	T20978	punct	;,depict
R6456	T20986	T20987	nsubj	asterisks,depict
R6457	T20987	T20978	ccomp	depict,depict
R6458	T20988	T20986	prep	in,asterisks
R6459	T20989	T20988	pobj	D,in
R646	T3107	T3105	amod	pulmonary,septation
R6460	T20990	T20991	det	the,absence
R6461	T20991	T20987	dobj	absence,depict
R6462	T20992	T20991	prep	of,absence
R6463	T20993	T20994	det	the,primordia
R6464	T20994	T20992	pobj	primordia,of
R6465	T20995	T20994	amod	thymic,primordia
R6466	T20996	T20978	punct	;,depict
R6467	T20997	T20978	nsubj	asterisks,depict
R6468	T20998	T20997	prep	in,asterisks
R6469	T20999	T20998	pobj	E,in
R647	T3108	T3107	punct	-,pulmonary
R6470	T21000	T20999	cc	and,E
R6471	T21001	T20999	conj	F,E
R6472	T21002	T21003	det	the,tongue
R6473	T21003	T20978	dobj	tongue,depict
R6474	T21004	T20978	punct	.,depict
R648	T3160	T3153	punct	", ",phosphorylation
R649	T3109	T3105	punct	", ",septation
R65	T572	T573	amod	pluripotent,cells
R650	T3110	T3105	prep	among,septation
R651	T3111	T3112	amod	other,defects
R652	T3161	T3162	dep	which,phosphorylate
R653	T3112	T3110	pobj	defects,among
R654	T3162	T3153	relcl	phosphorylate,phosphorylation
R655	T3113	T3112	amod	cardiac,defects
R656	T3163	T3162	punct	", ",phosphorylate
R657	T3114	T3115	punct	[,13
R658	T3164	T3162	prep	in,phosphorylate
R659	T3115	T3083	parataxis	13,shown
R66	T573	T571	pobj	cells,of
R660	T3116	T3115	nummod	12,13
R661	T3117	T3115	punct	",",13
R662	T3165	T3164	pobj	turn,in
R663	T3118	T3115	punct	],13
R664	T3119	T3083	punct	.,shown
R665	T3166	T3162	punct	", ",phosphorylate
R666	T3121	T3122	compound	TGF,β
R667	T3122	T3124	compound	β,ligands
R668	T3167	T3162	cc	and,phosphorylate
R669	T3123	T3122	punct	-,β
R67	T574	T575	amod	cardiac,crest
R670	T3124	T3126	nsubj	ligands,signal
R671	T3125	T3124	compound	subfamily,ligands
R672	T3168	T3162	conj	activate,phosphorylate
R673	T3127	T3126	prep	via,signal
R674	T3169	T3170	det	a,set
R675	T3128	T3129	det	a,complex
R676	T3129	T3127	pobj	complex,via
R677	T3130	T3129	compound	receptor,complex
R678	T3131	T3129	acl	composed,complex
R679	T3132	T3131	prep	of,composed
R68	T575	T573	compound	crest,cells
R680	T3170	T3168	dobj	set,activate
R681	T3133	T3134	nummod	two,receptors
R682	T3134	T3132	pobj	receptors,of
R683	T3135	T3134	nmod	type,receptors
R684	T3171	T3170	amod	specific,set
R685	T3136	T3135	nummod	II,type
R686	T3137	T3134	cc	and,receptors
R687	T3138	T3139	nummod	two,receptors
R688	T3172	T3170	prep	of,set
R689	T3139	T3134	conj	receptors,receptors
R69	T576	T575	amod	neural,crest
R690	T3140	T3139	nmod	type,receptors
R691	T3173	T3174	amod	downstream,molecules
R692	T3141	T3140	nummod	I,type
R693	T3142	T3143	punct	[,15
R694	T3174	T3172	pobj	molecules,of
R695	T3143	T3126	parataxis	15,signal
R696	T3144	T3143	nummod	14,15
R697	T3145	T3143	punct	",",15
R698	T3146	T3143	punct	],15
R699	T3175	T3174	compound	signaling,molecules
R70	T577	T573	compound	stem,cells
R700	T3147	T3126	punct	.,signal
R701	T3176	T3174	acl	called,molecules
R702	T3149	T3150	compound	Ligand,binding
R703	T3150	T3151	nsubj	binding,leads
R704	T3177	T3176	oprd	Smads,called
R705	T3152	T3151	prep	to,leads
R706	T3178	T3151	punct	.,leads
R707	T3180	T3181	prep	In,bind
R708	T3259	T3254	ccomp	are,is
R709	T3182	T3183	amod	general,terms
R71	T578	T556	punct	.,associated
R710	T3260	T3261	det	these,interactions
R711	T3261	T3259	nsubj	interactions,are
R712	T3262	T3261	compound	signaling,interactions
R713	T3183	T3180	pobj	terms,In
R714	T3263	T3264	advmod	more,complex
R715	T3184	T3181	punct	", ",bind
R716	T3264	T3259	acomp	complex,are
R717	T3265	T3266	advmod	in,vivo
R718	T3185	T3186	compound	TGF,βs
R719	T3266	T3259	advmod	vivo,are
R72	T580	T581	mark	While,implicated
R720	T3267	T3259	punct	", ",are
R721	T3268	T3269	advmod	possibly,allowing
R722	T3186	T3181	nsubj	βs,bind
R723	T3269	T3259	advcl	allowing,are
R724	T3270	T3269	dobj	formation,allowing
R725	T3271	T3270	prep	of,formation
R726	T3187	T3186	punct	-,βs
R727	T3272	T3273	amod	heterotetrameric,complexes
R728	T3273	T3271	pobj	complexes,of
R729	T3274	T3273	acl	composed,complexes
R73	T581	T591	advcl	implicated,understood
R730	T3275	T3274	prep	of,composed
R731	T3276	T3277	amod	different,receptors
R732	T3277	T3275	pobj	receptors,of
R733	T3188	T3181	prep	to,bind
R734	T3278	T3277	nmod	type,receptors
R735	T3279	T3278	nummod	II,type
R736	T3280	T3278	cc	and,type
R737	T3281	T3278	conj	type,type
R738	T3189	T3190	det	the,receptor
R739	T3282	T3281	nummod	I,type
R74	T582	T583	compound	TGF,β
R740	T3283	T3284	punct	[,16
R741	T3284	T3259	parataxis	16,are
R742	T3190	T3188	pobj	receptor,to
R743	T3285	T3284	punct	],16
R744	T3286	T3254	punct	.,is
R745	T3191	T3192	nmod	TGF,β
R746	T3288	T3289	prep	In,identified
R747	T3192	T3190	nmod	β,receptor
R748	T3290	T3288	pobj	addition,In
R749	T3291	T3289	punct	", ",identified
R75	T583	T585	compound	β,superfamily
R750	T3292	T3293	amod	recent,studies
R751	T3193	T3192	punct	-,β
R752	T3293	T3289	nsubj	studies,identified
R753	T3294	T3289	aux	have,identified
R754	T3295	T3296	amod	novel,ligands
R755	T3194	T3190	nmod	type,receptor
R756	T3296	T3289	dobj	ligands,identified
R757	T3297	T3298	compound	TGF,β
R758	T3298	T3300	npadvmod	β,related
R759	T3195	T3194	nummod	II,type
R76	T584	T583	punct	-,β
R760	T3299	T3298	punct	-,β
R761	T3300	T3296	amod	related,ligands
R762	T3301	T3300	punct	-,related
R763	T3196	T3190	punct	(,receptor
R764	T3197	T3190	appos	TGFβRII,receptor
R765	T3302	T3296	punct	", ",ligands
R766	T3303	T3304	dep	which,bind
R767	T3304	T3296	relcl	bind,ligands
R768	T3198	T3190	punct	),receptor
R769	T3305	T3304	aux	can,bind
R77	T585	T587	compound	superfamily,signaling
R770	T3306	T3304	prep	to,bind
R771	T3199	T3190	cc	and,receptor
R772	T3307	T3308	amod	entire,combinations
R773	T3308	T3306	pobj	combinations,to
R774	T3309	T3308	amod	different,combinations
R775	T3200	T3201	nmod	TGF,β
R776	T3310	T3308	prep	of,combinations
R777	T3311	T3310	pobj	receptors,of
R778	T3201	T3203	nmod	β,receptor
R779	T3312	T3289	punct	.,identified
R78	T586	T585	punct	-,superfamily
R780	T3202	T3201	punct	-,β
R781	T3314	T3315	prep	For,bind
R782	T3316	T3314	pobj	instance,For
R783	T3317	T3315	punct	", ",bind
R784	T3318	T3319	nmod	growth,factors
R785	T3319	T3315	nsubj	factors,bind
R786	T3203	T3190	conj	receptor,receptor
R787	T3320	T3318	cc	and,growth
R788	T3321	T3318	conj	differentiation,growth
R789	T3322	T3319	punct	(,factors
R79	T587	T581	nsubjpass	signaling,implicated
R790	T3323	T3319	appos	GDFs,factors
R791	T3204	T3203	nmod	type,receptor
R792	T3324	T3319	punct	),factors
R793	T3325	T3319	appos	8,factors
R794	T3205	T3204	nummod	I,type
R795	T3326	T3325	cc	and,8
R796	T3327	T3325	conj	9,8
R797	T3328	T3315	aux	can,bind
R798	T3206	T3203	punct	(,receptor
R799	T3329	T3315	prep	to,bind
R80	T588	T581	aux	has,implicated
R800	T3330	T3331	nmod	Activin,receptor
R801	T3331	T3329	pobj	receptor,to
R802	T3332	T3331	nmod	type,receptor
R803	T3207	T3203	appos	ALK5,receptor
R804	T3333	T3332	nummod	II,type
R805	T3334	T3331	cc	and,receptor
R806	T3208	T3181	punct	),bind
R807	T3335	T3331	conj	ALK5,receptor
R808	T3336	T3337	aux	to,activate
R809	T3337	T3315	advcl	activate,bind
R81	T589	T581	auxpass	been,implicated
R810	T3209	T3181	advcl	activating,bind
R811	T3338	T3339	compound	TGF,β
R812	T3339	T3341	compound	β,Smads
R813	T3340	T3339	punct	-,β
R814	T3210	T3211	compound	TGF,β
R815	T3341	T3337	dobj	Smads,activate
R816	T3342	T3343	punct	[,18
R817	T3211	T3213	compound	β,Smads
R818	T3343	T3315	parataxis	18,bind
R819	T3344	T3343	nummod	17,18
R82	T590	T581	advmod	strongly,implicated
R820	T3345	T3343	punct	",",18
R821	T3212	T3211	punct	-,β
R822	T3346	T3343	punct	],18
R823	T3347	T3315	punct	.,bind
R824	T3213	T3209	dobj	Smads,activating
R825	T3349	T3350	advmod	Therefore,hypothesized
R826	T3214	T3215	punct	(,2
R827	T3351	T3350	punct	", ",hypothesized
R828	T3352	T3350	nsubj	we,hypothesized
R829	T3353	T3354	mark	that,reveal
R83	T592	T581	prep	in,implicated
R830	T3215	T3209	parataxis	2,activating
R831	T3354	T3350	ccomp	reveal,hypothesized
R832	T3355	T3354	nsubj	deletion,reveal
R833	T3356	T3355	prep	of,deletion
R834	T3357	T3356	pobj	Alk5,of
R835	T3358	T3355	prep	in,deletion
R836	T3359	T3360	det	a,lineage
R837	T3216	T3215	cc	and,2
R838	T3360	T3358	pobj	lineage,in
R839	T3361	T3360	amod	specific,lineage
R84	T593	T594	amod	neural,cell
R840	T3362	T3360	compound	cell,lineage
R841	T3217	T3215	conj	3,2
R842	T3363	T3354	aux	should,reveal
R843	T3364	T3354	dobj	phenotypes,reveal
R844	T3218	T3215	punct	),2
R845	T3219	T3181	punct	", ",bind
R846	T3220	T3221	mark	while,bind
R847	T3221	T3181	advcl	bind,bind
R848	T3365	T3366	dep	which,seen
R849	T3366	T3364	relcl	seen,phenotypes
R85	T594	T596	compound	cell,development
R850	T3222	T3221	nsubj	BMPs,bind
R851	T3367	T3366	aux	can,seen
R852	T3368	T3366	neg	not,seen
R853	T3369	T3366	auxpass	be,seen
R854	T3223	T3221	prep	to,bind
R855	T3370	T3366	prep	in,seen
R856	T3371	T3372	amod	comparable,mutants
R857	T3224	T3225	det	the,ALK2
R858	T3372	T3370	pobj	mutants,in
R859	T3373	T3372	acl	lacking,mutants
R86	T595	T594	compound	crest,cell
R860	T3225	T3223	pobj	ALK2,to
R861	T3374	T3373	dobj	Tgfbr2,lacking
R862	T3375	T3350	punct	.,hypothesized
R863	T3226	T3225	nmod	BMP,ALK2
R864	T3377	T3378	advmod	Indeed,showed
R865	T3378	T3382	ccomp	showed,seen
R866	T3379	T3378	punct	", ",showed
R867	T3227	T3228	nmod	type,receptor
R868	T3380	T3378	nsubj	we,showed
R869	T3381	T3378	advmod	recently,showed
R87	T596	T592	pobj	development,in
R870	T3228	T3225	nmod	receptor,ALK2
R871	T3383	T3384	mark	that,display
R872	T3229	T3227	nummod	II,type
R873	T3230	T3228	cc	and,receptor
R874	T3384	T3378	ccomp	display,showed
R875	T3231	T3232	nmod	type,receptors
R876	T3385	T3386	amod	neural,cell
R877	T3386	T3388	npadvmod	cell,specific
R878	T3387	T3386	compound	crest,cell
R879	T3232	T3228	conj	receptors,receptor
R88	T597	T591	punct	", ",understood
R880	T3388	T3389	amod	specific,mutants
R881	T3389	T3384	nsubj	mutants,display
R882	T3233	T3231	nummod	I,type
R883	T3390	T3389	compound	Alk5,mutants
R884	T3391	T3392	det	a,spectrum
R885	T3392	T3384	dobj	spectrum,display
R886	T3393	T3392	amod	unique,spectrum
R887	T3394	T3392	prep	of,spectrum
R888	T3234	T3225	punct	", ",ALK2
R889	T3395	T3396	amod	craniofacial,defects
R89	T598	T599	det	the,mechanisms
R890	T3396	T3394	pobj	defects,of
R891	T3397	T3396	amod	developmental,defects
R892	T3235	T3225	punct	-,ALK2
R893	T3398	T3396	punct	", ",defects
R894	T3399	T3400	advmod	e.g.,snout
R895	T3236	T3225	nummod	3,ALK2
R896	T3400	T3396	appos	snout,defects
R897	T3401	T3400	punct	", ",snout
R898	T3237	T3225	punct	", ",ALK2
R899	T3402	T3400	compound	cleft,snout
R90	T599	T591	nsubjpass	mechanisms,understood
R900	T3403	T3400	cc	and,snout
R901	T3404	T3405	amod	severe,hypoplasia
R902	T3238	T3225	cc	or,ALK2
R903	T3405	T3400	conj	hypoplasia,snout
R904	T3406	T3405	amod	mandibular,hypoplasia
R905	T3407	T3408	punct	[,19
R906	T3239	T3225	conj	6,ALK2
R907	T3408	T3378	parataxis	19,showed
R908	T3409	T3408	punct	],19
R909	T3240	T3221	punct	", ",bind
R91	T600	T599	amod	detailed,mechanisms
R910	T3410	T3382	punct	;,seen
R911	T3411	T3412	det	these,phenotypes
R912	T3412	T3382	nsubjpass	phenotypes,seen
R913	T3241	T3221	advcl	activating,bind
R914	T3413	T3382	auxpass	were,seen
R915	T3414	T3382	neg	not,seen
R916	T3242	T3243	compound	BMP,Smads
R917	T3415	T3382	prep	in,seen
R918	T3416	T3417	amod	corresponding,mutants
R919	T3417	T3415	pobj	mutants,in
R92	T601	T599	amod	molecular,mechanisms
R920	T3243	T3241	dobj	Smads,activating
R921	T3418	T3417	compound	Tgfbr2,mutants
R922	T3419	T3420	punct	[,20
R923	T3420	T3382	parataxis	20,seen
R924	T3244	T3245	punct	(,1
R925	T3421	T3420	punct	],20
R926	T3422	T3382	punct	.,seen
R927	T3245	T3181	parataxis	1,bind
R928	T3424	T3425	aux	To,determine
R929	T3425	T3426	advcl	determine,focused
R93	T602	T599	compound	signaling,mechanisms
R930	T3246	T3245	punct	", ",1
R931	T3427	T3425	punct	", ",determine
R932	T3247	T3245	conj	5,1
R933	T3428	T3429	mark	whether,mediate
R934	T3429	T3425	ccomp	mediate,determine
R935	T3430	T3429	nsubj	ALK5,mediate
R936	T3248	T3247	cc	and,5
R937	T3431	T3429	aux	would,mediate
R938	T3432	T3429	advmod	also,mediate
R939	T3433	T3434	amod	unique,events
R94	T603	T604	advmod	in,vivo
R940	T3434	T3429	dobj	events,mediate
R941	T3249	T3247	conj	8,5
R942	T3435	T3434	amod	non-redundant,events
R943	T3436	T3434	compound	signaling,events
R944	T3437	T3429	prep	in,mediate
R945	T3250	T3245	punct	),1
R946	T3438	T3439	amod	cardiac,crest
R947	T3439	T3441	compound	crest,cells
R948	T3440	T3439	amod	neural,crest
R949	T3251	T3181	punct	.,bind
R95	T604	T599	advmod	vivo,mechanisms
R950	T3441	T3437	pobj	cells,in
R951	T3442	T3426	punct	", ",focused
R952	T3443	T3426	nsubj	we,focused
R953	T3253	T3254	advmod	However,is
R954	T3444	T3426	prep	on,focused
R955	T3445	T3446	amod	cardiac,phenotypes
R956	T3255	T3254	punct	", ",is
R957	T3446	T3444	pobj	phenotypes,on
R958	T3447	T3445	cc	and,cardiac
R959	T3256	T3254	nsubj	it,is
R96	T605	T591	auxpass	are,understood
R960	T3448	T3445	conj	pharyngeal,cardiac
R961	T3449	T3446	prep	of,phenotypes
R962	T3450	T3451	compound	mouse,embryos
R963	T3257	T3254	acomp	likely,is
R964	T3451	T3449	pobj	embryos,of
R965	T3452	T3451	acl	lacking,embryos
R966	T3453	T3452	dobj	Alk5,lacking
R967	T3258	T3259	mark	that,are
R968	T3454	T3455	advmod	specifically,in
R969	T3455	T3426	prep	in,focused
R97	T606	T591	advmod	still,understood
R970	T3471	T3465	pobj	mutants,in
R971	T3456	T3457	amod	neural,cells
R972	T3457	T3455	pobj	cells,in
R973	T3458	T3457	compound	crest,cells
R974	T3459	T3426	punct	.,focused
R975	T3472	T3464	punct	", ",fail
R976	T3461	T3462	nsubj	We,discovered
R977	T3463	T3464	mark	that,fail
R978	T3473	T3474	amod	pharyngeal,organs
R979	T3464	T3462	ccomp	fail,discovered
R98	T607	T591	advmod	poorly,understood
R980	T3465	T3464	prep	in,fail
R981	T3474	T3464	nsubj	organs,fail
R982	T3466	T3467	compound	Alk5,Cre
R983	T3467	T3471	compound	Cre,mutants
R984	T3468	T3467	punct	/,Cre
R985	T3475	T3474	punct	(,organs
R986	T3469	T3467	compound	Wnt1,Cre
R987	T3470	T3467	punct	-,Cre
R988	T3476	T3474	appos	thymus,organs
R989	T3477	T3476	cc	and,thymus
R99	T608	T591	punct	.,understood
R990	T3478	T3476	conj	parathyroid,thymus
R991	T3577	T3541	punct	.,differ
R992	T3479	T3464	punct	),fail
R993	T3480	T3481	aux	to,migrate
R994	T3481	T3464	xcomp	migrate,fail
R995	T3482	T3481	advmod	appropriately,migrate
R996	T3483	T3462	punct	.,discovered
R997	T3485	T3486	advmod	Moreover,display
R998	T3487	T3486	punct	", ",display
R999	T3488	T3489	det	the,embryos

PMC:1635039 JSONTXT 7 Projects

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PMC:1635039 JSON TXT 7 Projects