| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T8888 |
0-6 |
NN |
denotes |
Retina |
| T8889 |
7-18 |
NNS |
denotes |
dissections |
| T8891 |
18-20 |
, |
denotes |
, |
| T8892 |
20-26 |
JJ |
denotes |
radial |
| T8893 |
27-39 |
NNS |
denotes |
cryosections |
| T8894 |
40-41 |
-LRB- |
denotes |
( |
| T8896 |
41-42 |
SYM |
denotes |
~ |
| T8897 |
42-43 |
CD |
denotes |
6 |
| T8895 |
44-46 |
NNS |
denotes |
μm |
| T8898 |
46-47 |
-RRB- |
denotes |
) |
| T8899 |
47-49 |
, |
denotes |
, |
| T8900 |
49-52 |
CC |
denotes |
and |
| T8901 |
53-73 |
NN |
denotes |
immunohistochemistry |
| T8902 |
74-84 |
NNS |
denotes |
procedures |
| T8903 |
85-89 |
VBD |
denotes |
were |
| T8890 |
90-97 |
VBN |
denotes |
carried |
| T8904 |
98-101 |
RP |
denotes |
out |
| T8905 |
102-104 |
IN |
denotes |
as |
| T8906 |
105-114 |
VBN |
denotes |
described |
| T8907 |
115-124 |
RB |
denotes |
elsewhere |
| T8908 |
125-126 |
-LRB- |
denotes |
[ |
| T8909 |
126-128 |
CD |
denotes |
19 |
| T8910 |
128-129 |
-RRB- |
denotes |
] |
| T8911 |
129-130 |
. |
denotes |
. |
| T8912 |
130-275 |
sentence |
denotes |
Brains from 3- to 5-mo-old C57Bl/6 mice were fixed overnight in 2% paraformaldehyde, infused with 30% sucrose, and processed for cryosectioning. |
| T8913 |
131-137 |
NNS |
denotes |
Brains |
| T8915 |
138-142 |
IN |
denotes |
from |
| T8916 |
143-144 |
CD |
denotes |
3 |
| T8918 |
144-145 |
HYPH |
denotes |
- |
| T8919 |
146-148 |
IN |
denotes |
to |
| T8917 |
149-150 |
CD |
denotes |
5 |
| T8921 |
150-151 |
HYPH |
denotes |
- |
| T8920 |
151-153 |
NN |
denotes |
mo |
| T8923 |
153-154 |
HYPH |
denotes |
- |
| T8922 |
154-157 |
JJ |
denotes |
old |
| T8925 |
158-163 |
NN |
denotes |
C57Bl |
| T8926 |
163-164 |
HYPH |
denotes |
/ |
| T8927 |
164-165 |
CD |
denotes |
6 |
| T8924 |
166-170 |
NNS |
denotes |
mice |
| T8928 |
171-175 |
VBD |
denotes |
were |
| T8914 |
176-181 |
VBN |
denotes |
fixed |
| T8929 |
182-191 |
RB |
denotes |
overnight |
| T8930 |
192-194 |
IN |
denotes |
in |
| T8931 |
195-196 |
CD |
denotes |
2 |
| T8932 |
196-197 |
NN |
denotes |
% |
| T8933 |
198-214 |
NN |
denotes |
paraformaldehyde |
| T8934 |
214-216 |
, |
denotes |
, |
| T8935 |
216-223 |
VBN |
denotes |
infused |
| T8936 |
224-228 |
IN |
denotes |
with |
| T8937 |
229-231 |
CD |
denotes |
30 |
| T8938 |
231-232 |
NN |
denotes |
% |
| T8939 |
233-240 |
NN |
denotes |
sucrose |
| T8940 |
240-242 |
, |
denotes |
, |
| T8941 |
242-245 |
CC |
denotes |
and |
| T8942 |
246-255 |
VBN |
denotes |
processed |
| T8943 |
256-259 |
IN |
denotes |
for |
| T8944 |
260-274 |
VBG |
denotes |
cryosectioning |
| T8945 |
274-275 |
. |
denotes |
. |
| T8946 |
275-353 |
sentence |
denotes |
Primary brain neurons and glial cells were prepared from the cerebral cortex. |
| T8947 |
276-283 |
JJ |
denotes |
Primary |
| T8949 |
284-289 |
NN |
denotes |
brain |
| T8948 |
290-297 |
NNS |
denotes |
neurons |
| T8951 |
298-301 |
CC |
denotes |
and |
| T8952 |
302-307 |
JJ |
denotes |
glial |
| T8953 |
308-313 |
NNS |
denotes |
cells |
| T8954 |
314-318 |
VBD |
denotes |
were |
| T8950 |
319-327 |
VBN |
denotes |
prepared |
| T8955 |
328-332 |
IN |
denotes |
from |
| T8956 |
333-336 |
DT |
denotes |
the |
| T8958 |
337-345 |
JJ |
denotes |
cerebral |
| T8957 |
346-352 |
NN |
denotes |
cortex |
| T8959 |
352-353 |
. |
denotes |
. |
| T8960 |
353-425 |
sentence |
denotes |
This was macerated in the Hank's Balanced Salt solution and triturated. |
| T8961 |
354-358 |
DT |
denotes |
This |
| T8963 |
359-362 |
VBD |
denotes |
was |
| T8962 |
363-372 |
VBN |
denotes |
macerated |
| T8964 |
373-375 |
IN |
denotes |
in |
| T8965 |
376-379 |
DT |
denotes |
the |
| T8967 |
380-384 |
NNP |
denotes |
Hank |
| T8969 |
384-386 |
POS |
denotes |
's |
| T8970 |
387-395 |
NNP |
denotes |
Balanced |
| T8968 |
396-400 |
NNP |
denotes |
Salt |
| T8966 |
401-409 |
NN |
denotes |
solution |
| T8971 |
410-413 |
CC |
denotes |
and |
| T8972 |
414-424 |
VBN |
denotes |
triturated |
| T8973 |
424-425 |
. |
denotes |
. |
| T8974 |
425-662 |
sentence |
denotes |
Primary cells were cultured in DMEM (GIBCO, San Diego, California, United States) and collagen-coated 35-mm glass bottom culture dishes (MatTek Corporation, Ashland, Maine) at 5% CO2/37 °C for ~2 d and processed for immunocytochemistry. |
| T8975 |
426-433 |
JJ |
denotes |
Primary |
| T8976 |
434-439 |
NNS |
denotes |
cells |
| T8978 |
440-444 |
VBD |
denotes |
were |
| T8977 |
445-453 |
VBN |
denotes |
cultured |
| T8979 |
454-456 |
IN |
denotes |
in |
| T8980 |
457-461 |
NN |
denotes |
DMEM |
| T8981 |
462-463 |
-LRB- |
denotes |
( |
| T8982 |
463-468 |
NNP |
denotes |
GIBCO |
| T8983 |
468-470 |
, |
denotes |
, |
| T8984 |
470-473 |
NNP |
denotes |
San |
| T8985 |
474-479 |
NNP |
denotes |
Diego |
| T8986 |
479-481 |
, |
denotes |
, |
| T8987 |
481-491 |
NNP |
denotes |
California |
| T8988 |
491-493 |
, |
denotes |
, |
| T8989 |
493-499 |
NNP |
denotes |
United |
| T8990 |
500-506 |
NNP |
denotes |
States |
| T8991 |
506-507 |
-RRB- |
denotes |
) |
| T8992 |
508-511 |
CC |
denotes |
and |
| T8993 |
512-520 |
NN |
denotes |
collagen |
| T8995 |
520-521 |
HYPH |
denotes |
- |
| T8994 |
521-527 |
VBN |
denotes |
coated |
| T8997 |
528-530 |
CD |
denotes |
35 |
| T8999 |
530-531 |
HYPH |
denotes |
- |
| T8998 |
531-533 |
NN |
denotes |
mm |
| T9000 |
534-539 |
NN |
denotes |
glass |
| T9001 |
540-546 |
NN |
denotes |
bottom |
| T9002 |
547-554 |
NN |
denotes |
culture |
| T8996 |
555-561 |
NNS |
denotes |
dishes |
| T9003 |
562-563 |
-LRB- |
denotes |
( |
| T9005 |
563-569 |
NNP |
denotes |
MatTek |
| T9004 |
570-581 |
NNP |
denotes |
Corporation |
| T9006 |
581-583 |
, |
denotes |
, |
| T9007 |
583-590 |
NNP |
denotes |
Ashland |
| T9008 |
590-592 |
, |
denotes |
, |
| T9009 |
592-597 |
NNP |
denotes |
Maine |
| T9010 |
597-598 |
-RRB- |
denotes |
) |
| T9011 |
599-601 |
IN |
denotes |
at |
| T9012 |
602-603 |
CD |
denotes |
5 |
| T9013 |
603-604 |
NN |
denotes |
% |
| T9014 |
605-608 |
NN |
denotes |
CO2 |
| T9015 |
608-609 |
HYPH |
denotes |
/ |
| T9016 |
609-611 |
CD |
denotes |
37 |
| T9017 |
612-614 |
NN |
denotes |
°C |
| T9018 |
615-618 |
IN |
denotes |
for |
| T9019 |
619-620 |
SYM |
denotes |
~ |
| T9020 |
620-621 |
CD |
denotes |
2 |
| T9021 |
622-623 |
NNS |
denotes |
d |
| T9022 |
624-627 |
CC |
denotes |
and |
| T9023 |
628-637 |
VBN |
denotes |
processed |
| T9024 |
638-641 |
IN |
denotes |
for |
| T9025 |
642-661 |
NN |
denotes |
immunocytochemistry |
| T9026 |
661-662 |
. |
denotes |
. |
| T9027 |
662-724 |
sentence |
denotes |
Primary antibodies were used at concentrations ~2.5–10 μg/ml. |
| T9028 |
663-670 |
JJ |
denotes |
Primary |
| T9029 |
671-681 |
NNS |
denotes |
antibodies |
| T9031 |
682-686 |
VBD |
denotes |
were |
| T9030 |
687-691 |
VBN |
denotes |
used |
| T9032 |
692-694 |
IN |
denotes |
at |
| T9033 |
695-709 |
NNS |
denotes |
concentrations |
| T9034 |
710-711 |
SYM |
denotes |
~ |
| T9036 |
711-714 |
CD |
denotes |
2.5 |
| T9037 |
714-715 |
SYM |
denotes |
– |
| T9035 |
715-717 |
CD |
denotes |
10 |
| T9038 |
718-720 |
NN |
denotes |
μg |
| T9039 |
720-721 |
SYM |
denotes |
/ |
| T9040 |
721-723 |
NN |
denotes |
ml |
| T9041 |
723-724 |
. |
denotes |
. |
| T9042 |
724-853 |
sentence |
denotes |
Alexa 488- and Alexa 594-conjugated secondary antibodies (2.5 μg/ml) (Molecular Probes) were used for visualization of proteins. |
| T9043 |
725-730 |
NNP |
denotes |
Alexa |
| T9045 |
731-734 |
CD |
denotes |
488 |
| T9046 |
734-735 |
HYPH |
denotes |
- |
| T9047 |
736-739 |
CC |
denotes |
and |
| T9048 |
740-745 |
NNP |
denotes |
Alexa |
| T9049 |
746-749 |
JJ |
denotes |
594 |
| T9050 |
749-750 |
HYPH |
denotes |
- |
| T9044 |
750-760 |
JJ |
denotes |
conjugated |
| T9052 |
761-770 |
JJ |
denotes |
secondary |
| T9051 |
771-781 |
NNS |
denotes |
antibodies |
| T9054 |
782-783 |
-LRB- |
denotes |
( |
| T9056 |
783-786 |
CD |
denotes |
2.5 |
| T9055 |
787-789 |
NNS |
denotes |
μg |
| T9057 |
789-790 |
SYM |
denotes |
/ |
| T9058 |
790-792 |
NN |
denotes |
ml |
| T9059 |
792-793 |
-RRB- |
denotes |
) |
| T9060 |
794-795 |
-LRB- |
denotes |
( |
| T9062 |
795-804 |
NNP |
denotes |
Molecular |
| T9061 |
805-811 |
NNP |
denotes |
Probes |
| T9063 |
811-812 |
-RRB- |
denotes |
) |
| T9064 |
813-817 |
VBD |
denotes |
were |
| T9053 |
818-822 |
VBN |
denotes |
used |
| T9065 |
823-826 |
IN |
denotes |
for |
| T9066 |
827-840 |
NN |
denotes |
visualization |
| T9067 |
841-843 |
IN |
denotes |
of |
| T9068 |
844-852 |
NN |
denotes |
proteins |
| T9069 |
852-853 |
. |
denotes |
. |
| T9070 |
853-948 |
sentence |
denotes |
Crossover of fluorescent probes, background, and autofluorescence were found to be negligible. |
| T9071 |
854-863 |
NN |
denotes |
Crossover |
| T9073 |
864-866 |
IN |
denotes |
of |
| T9074 |
867-878 |
JJ |
denotes |
fluorescent |
| T9075 |
879-885 |
NNS |
denotes |
probes |
| T9076 |
885-887 |
, |
denotes |
, |
| T9077 |
887-897 |
NN |
denotes |
background |
| T9078 |
897-899 |
, |
denotes |
, |
| T9079 |
899-902 |
CC |
denotes |
and |
| T9080 |
903-919 |
NN |
denotes |
autofluorescence |
| T9081 |
920-924 |
VBD |
denotes |
were |
| T9072 |
925-930 |
VBN |
denotes |
found |
| T9082 |
931-933 |
TO |
denotes |
to |
| T9083 |
934-936 |
VB |
denotes |
be |
| T9084 |
937-947 |
JJ |
denotes |
negligible |
| T9085 |
947-948 |
. |
denotes |
. |
| T9086 |
948-1186 |
sentence |
denotes |
Visualization of specimens and localization of proteins were carried out by wide-field epifluorescence microscopy on Nikon (Tokyo, Japan) E600 upright and TE2000U inverted research microscopes equipped with similar Apochromat objectives. |
| T9087 |
949-962 |
NN |
denotes |
Visualization |
| T9089 |
963-965 |
IN |
denotes |
of |
| T9090 |
966-975 |
NNS |
denotes |
specimens |
| T9091 |
976-979 |
CC |
denotes |
and |
| T9092 |
980-992 |
NN |
denotes |
localization |
| T9093 |
993-995 |
IN |
denotes |
of |
| T9094 |
996-1004 |
NN |
denotes |
proteins |
| T9095 |
1005-1009 |
VBD |
denotes |
were |
| T9088 |
1010-1017 |
VBN |
denotes |
carried |
| T9096 |
1018-1021 |
RP |
denotes |
out |
| T9097 |
1022-1024 |
IN |
denotes |
by |
| T9098 |
1025-1029 |
JJ |
denotes |
wide |
| T9100 |
1029-1030 |
HYPH |
denotes |
- |
| T9099 |
1030-1035 |
NN |
denotes |
field |
| T9102 |
1036-1051 |
NN |
denotes |
epifluorescence |
| T9101 |
1052-1062 |
NN |
denotes |
microscopy |
| T9103 |
1063-1065 |
IN |
denotes |
on |
| T9104 |
1066-1071 |
NNP |
denotes |
Nikon |
| T9106 |
1072-1073 |
-LRB- |
denotes |
( |
| T9107 |
1073-1078 |
NNP |
denotes |
Tokyo |
| T9108 |
1078-1080 |
, |
denotes |
, |
| T9109 |
1080-1085 |
NNP |
denotes |
Japan |
| T9110 |
1085-1086 |
-RRB- |
denotes |
) |
| T9111 |
1087-1091 |
NN |
denotes |
E600 |
| T9105 |
1092-1099 |
NN |
denotes |
upright |
| T9112 |
1100-1103 |
CC |
denotes |
and |
| T9113 |
1104-1111 |
NN |
denotes |
TE2000U |
| T9115 |
1112-1120 |
VBN |
denotes |
inverted |
| T9116 |
1121-1129 |
NN |
denotes |
research |
| T9114 |
1130-1141 |
NNS |
denotes |
microscopes |
| T9117 |
1142-1150 |
VBN |
denotes |
equipped |
| T9118 |
1151-1155 |
IN |
denotes |
with |
| T9119 |
1156-1163 |
JJ |
denotes |
similar |
| T9121 |
1164-1174 |
NN |
denotes |
Apochromat |
| T9120 |
1175-1185 |
NNS |
denotes |
objectives |
| T9122 |
1185-1186 |
. |
denotes |
. |
| T9123 |
1186-1393 |
sentence |
denotes |
Images with the Nikon E600 were acquired with a SPOT-RT digital camera coupled to the microscope and driven by SPOT Imaging v4.0 software (Diagnostic Instruments, Sterling Heights, Michigan, United States). |
| T9124 |
1187-1193 |
NNS |
denotes |
Images |
| T9126 |
1194-1198 |
IN |
denotes |
with |
| T9127 |
1199-1202 |
DT |
denotes |
the |
| T9129 |
1203-1208 |
NNP |
denotes |
Nikon |
| T9128 |
1209-1213 |
NN |
denotes |
E600 |
| T9130 |
1214-1218 |
VBD |
denotes |
were |
| T9125 |
1219-1227 |
VBN |
denotes |
acquired |
| T9131 |
1228-1232 |
IN |
denotes |
with |
| T9132 |
1233-1234 |
DT |
denotes |
a |
| T9134 |
1235-1239 |
NN |
denotes |
SPOT |
| T9136 |
1239-1240 |
HYPH |
denotes |
- |
| T9135 |
1240-1242 |
NN |
denotes |
RT |
| T9137 |
1243-1250 |
JJ |
denotes |
digital |
| T9133 |
1251-1257 |
NN |
denotes |
camera |
| T9138 |
1258-1265 |
VBN |
denotes |
coupled |
| T9139 |
1266-1268 |
IN |
denotes |
to |
| T9140 |
1269-1272 |
DT |
denotes |
the |
| T9141 |
1273-1283 |
NN |
denotes |
microscope |
| T9142 |
1284-1287 |
CC |
denotes |
and |
| T9143 |
1288-1294 |
VBN |
denotes |
driven |
| T9144 |
1295-1297 |
IN |
denotes |
by |
| T9145 |
1298-1302 |
NN |
denotes |
SPOT |
| T9147 |
1303-1310 |
NN |
denotes |
Imaging |
| T9146 |
1311-1315 |
NN |
denotes |
v4.0 |
| T9148 |
1316-1324 |
NN |
denotes |
software |
| T9149 |
1325-1326 |
-LRB- |
denotes |
( |
| T9151 |
1326-1336 |
NNP |
denotes |
Diagnostic |
| T9150 |
1337-1348 |
NNPS |
denotes |
Instruments |
| T9152 |
1348-1350 |
, |
denotes |
, |
| T9153 |
1350-1358 |
NNP |
denotes |
Sterling |
| T9154 |
1359-1366 |
NNP |
denotes |
Heights |
| T9155 |
1366-1368 |
, |
denotes |
, |
| T9156 |
1368-1376 |
NNP |
denotes |
Michigan |
| T9157 |
1376-1378 |
, |
denotes |
, |
| T9158 |
1378-1384 |
NNP |
denotes |
United |
| T9159 |
1385-1391 |
NNP |
denotes |
States |
| T9160 |
1391-1392 |
-RRB- |
denotes |
) |
| T9161 |
1392-1393 |
. |
denotes |
. |
| T9162 |
1393-1505 |
sentence |
denotes |
All images were captured at nonsaturating integration levels, 12-bit mono black/white, and then pseudo-colored. |
| T9163 |
1394-1397 |
DT |
denotes |
All |
| T9164 |
1398-1404 |
NNS |
denotes |
images |
| T9166 |
1405-1409 |
VBD |
denotes |
were |
| T9165 |
1410-1418 |
VBN |
denotes |
captured |
| T9167 |
1419-1421 |
IN |
denotes |
at |
| T9168 |
1422-1435 |
NN |
denotes |
nonsaturating |
| T9170 |
1436-1447 |
NN |
denotes |
integration |
| T9169 |
1448-1454 |
NNS |
denotes |
levels |
| T9171 |
1454-1456 |
, |
denotes |
, |
| T9172 |
1456-1458 |
CD |
denotes |
12 |
| T9174 |
1458-1459 |
HYPH |
denotes |
- |
| T9173 |
1459-1462 |
JJ |
denotes |
bit |
| T9176 |
1463-1467 |
NN |
denotes |
mono |
| T9177 |
1468-1473 |
JJ |
denotes |
black |
| T9178 |
1473-1474 |
HYPH |
denotes |
/ |
| T9175 |
1474-1479 |
JJ |
denotes |
white |
| T9179 |
1479-1481 |
, |
denotes |
, |
| T9180 |
1481-1484 |
CC |
denotes |
and |
| T9181 |
1485-1489 |
RB |
denotes |
then |
| T9182 |
1490-1504 |
VBN |
denotes |
pseudo-colored |
| T9183 |
1504-1505 |
. |
denotes |
. |
| T9184 |
1505-1901 |
sentence |
denotes |
Protein colocalization analysis was carried out on the Nikon TE2000U microscope equipped with appropriate excitation and emission filter wheels, cube filters, 100 W mercury light source, Nomarski/DIC, and Plan Apochromat optics (100×, 60×, and 40× oil objectives with NA of 1.4, 40 ×LWD, and 20 ×LWD objectives and encoded motorized Z-Stage (Prior Scientific, Rockland, Maryland, United States). |
| T9185 |
1506-1513 |
NN |
denotes |
Protein |
| T9186 |
1514-1528 |
NN |
denotes |
colocalization |
| T9187 |
1529-1537 |
NN |
denotes |
analysis |
| T9189 |
1538-1541 |
VBD |
denotes |
was |
| T9188 |
1542-1549 |
VBN |
denotes |
carried |
| T9190 |
1550-1553 |
RP |
denotes |
out |
| T9191 |
1554-1556 |
IN |
denotes |
on |
| T9192 |
1557-1560 |
DT |
denotes |
the |
| T9194 |
1561-1566 |
NNP |
denotes |
Nikon |
| T9195 |
1567-1574 |
NN |
denotes |
TE2000U |
| T9193 |
1575-1585 |
NN |
denotes |
microscope |
| T9196 |
1586-1594 |
VBN |
denotes |
equipped |
| T9197 |
1595-1599 |
IN |
denotes |
with |
| T9198 |
1600-1611 |
JJ |
denotes |
appropriate |
| T9200 |
1612-1622 |
NN |
denotes |
excitation |
| T9201 |
1623-1626 |
CC |
denotes |
and |
| T9202 |
1627-1635 |
NN |
denotes |
emission |
| T9203 |
1636-1642 |
NN |
denotes |
filter |
| T9199 |
1643-1649 |
NNS |
denotes |
wheels |
| T9204 |
1649-1651 |
, |
denotes |
, |
| T9205 |
1651-1655 |
NN |
denotes |
cube |
| T9206 |
1656-1663 |
NNS |
denotes |
filters |
| T9207 |
1663-1665 |
, |
denotes |
, |
| T9208 |
1665-1668 |
CD |
denotes |
100 |
| T9209 |
1669-1670 |
NNP |
denotes |
W |
| T9211 |
1671-1678 |
NN |
denotes |
mercury |
| T9212 |
1679-1684 |
NN |
denotes |
light |
| T9210 |
1685-1691 |
NN |
denotes |
source |
| T9213 |
1691-1693 |
, |
denotes |
, |
| T9214 |
1693-1701 |
NNP |
denotes |
Nomarski |
| T9216 |
1701-1702 |
HYPH |
denotes |
/ |
| T9215 |
1702-1705 |
NNP |
denotes |
DIC |
| T9218 |
1705-1707 |
, |
denotes |
, |
| T9219 |
1707-1710 |
CC |
denotes |
and |
| T9220 |
1711-1715 |
NNP |
denotes |
Plan |
| T9221 |
1716-1726 |
NNP |
denotes |
Apochromat |
| T9217 |
1727-1733 |
NNS |
denotes |
optics |
| T9222 |
1734-1735 |
-LRB- |
denotes |
( |
| T9223 |
1735-1738 |
CD |
denotes |
100 |
| T9225 |
1738-1739 |
SYM |
denotes |
× |
| T9226 |
1739-1741 |
, |
denotes |
, |
| T9227 |
1741-1743 |
CD |
denotes |
60 |
| T9228 |
1743-1744 |
SYM |
denotes |
× |
| T9229 |
1744-1746 |
, |
denotes |
, |
| T9230 |
1746-1749 |
CC |
denotes |
and |
| T9231 |
1750-1752 |
CD |
denotes |
40 |
| T9232 |
1752-1753 |
SYM |
denotes |
× |
| T9233 |
1754-1757 |
NN |
denotes |
oil |
| T9224 |
1758-1768 |
NNS |
denotes |
objectives |
| T9234 |
1769-1773 |
IN |
denotes |
with |
| T9235 |
1774-1776 |
NN |
denotes |
NA |
| T9236 |
1777-1779 |
IN |
denotes |
of |
| T9237 |
1780-1783 |
CD |
denotes |
1.4 |
| T9238 |
1783-1785 |
, |
denotes |
, |
| T9239 |
1785-1787 |
CD |
denotes |
40 |
| T9241 |
1788-1789 |
SYM |
denotes |
× |
| T9240 |
1789-1792 |
NN |
denotes |
LWD |
| T9243 |
1792-1794 |
, |
denotes |
, |
| T9244 |
1794-1797 |
CC |
denotes |
and |
| T9245 |
1798-1800 |
CD |
denotes |
20 |
| T9247 |
1801-1802 |
SYM |
denotes |
× |
| T9246 |
1802-1805 |
NN |
denotes |
LWD |
| T9242 |
1806-1816 |
NNS |
denotes |
objectives |
| T9248 |
1817-1820 |
CC |
denotes |
and |
| T9249 |
1821-1828 |
VBN |
denotes |
encoded |
| T9251 |
1829-1838 |
VBN |
denotes |
motorized |
| T9252 |
1839-1840 |
NNP |
denotes |
Z |
| T9253 |
1840-1841 |
HYPH |
denotes |
- |
| T9250 |
1841-1846 |
NNP |
denotes |
Stage |
| T9254 |
1847-1848 |
-LRB- |
denotes |
( |
| T9256 |
1848-1853 |
NNP |
denotes |
Prior |
| T9255 |
1854-1864 |
NNP |
denotes |
Scientific |
| T9257 |
1864-1866 |
, |
denotes |
, |
| T9258 |
1866-1874 |
NNP |
denotes |
Rockland |
| T9259 |
1874-1876 |
, |
denotes |
, |
| T9260 |
1876-1884 |
NNP |
denotes |
Maryland |
| T9261 |
1884-1886 |
, |
denotes |
, |
| T9262 |
1886-1892 |
NNP |
denotes |
United |
| T9263 |
1893-1899 |
NNP |
denotes |
States |
| T9264 |
1899-1900 |
-RRB- |
denotes |
) |
| T9265 |
1900-1901 |
. |
denotes |
. |
| T9266 |
1901-2053 |
sentence |
denotes |
Images with the inverted Nikon TE2000U microscope were obtained using a CCD camera (CoolSNAP HQ; Roper Scientific, Trenton, New Jersey, United States). |
| T9267 |
1902-1908 |
NNS |
denotes |
Images |
| T9269 |
1909-1913 |
IN |
denotes |
with |
| T9270 |
1914-1917 |
DT |
denotes |
the |
| T9272 |
1918-1926 |
VBN |
denotes |
inverted |
| T9273 |
1927-1932 |
NNP |
denotes |
Nikon |
| T9274 |
1933-1940 |
NN |
denotes |
TE2000U |
| T9271 |
1941-1951 |
NN |
denotes |
microscope |
| T9275 |
1952-1956 |
VBD |
denotes |
were |
| T9268 |
1957-1965 |
VBN |
denotes |
obtained |
| T9276 |
1966-1971 |
VBG |
denotes |
using |
| T9277 |
1972-1973 |
DT |
denotes |
a |
| T9279 |
1974-1977 |
NN |
denotes |
CCD |
| T9278 |
1978-1984 |
NN |
denotes |
camera |
| T9280 |
1985-1986 |
-LRB- |
denotes |
( |
| T9281 |
1986-1994 |
NNP |
denotes |
CoolSNAP |
| T9282 |
1995-1997 |
NNP |
denotes |
HQ |
| T9283 |
1997-1998 |
: |
denotes |
; |
| T9285 |
1999-2004 |
NNP |
denotes |
Roper |
| T9284 |
2005-2015 |
NNP |
denotes |
Scientific |
| T9286 |
2015-2017 |
, |
denotes |
, |
| T9287 |
2017-2024 |
NNP |
denotes |
Trenton |
| T9288 |
2024-2026 |
, |
denotes |
, |
| T9289 |
2026-2029 |
NNP |
denotes |
New |
| T9290 |
2030-2036 |
NNP |
denotes |
Jersey |
| T9291 |
2036-2038 |
, |
denotes |
, |
| T9292 |
2038-2044 |
NNP |
denotes |
United |
| T9293 |
2045-2051 |
NNP |
denotes |
States |
| T9294 |
2051-2052 |
-RRB- |
denotes |
) |
| T9295 |
2052-2053 |
. |
denotes |
. |
| T9296 |
2053-2209 |
sentence |
denotes |
Images acquired under identical acquisition parameters were analyzed using Metamorph Software v6.2 (Universal Imaging, Glendale, Wisconsin, United States). |
| T9297 |
2054-2060 |
NNS |
denotes |
Images |
| T9299 |
2061-2069 |
VBN |
denotes |
acquired |
| T9300 |
2070-2075 |
IN |
denotes |
under |
| T9301 |
2076-2085 |
JJ |
denotes |
identical |
| T9303 |
2086-2097 |
NN |
denotes |
acquisition |
| T9302 |
2098-2108 |
NNS |
denotes |
parameters |
| T9304 |
2109-2113 |
VBD |
denotes |
were |
| T9298 |
2114-2122 |
VBN |
denotes |
analyzed |
| T9305 |
2123-2128 |
VBG |
denotes |
using |
| T9306 |
2129-2138 |
NNP |
denotes |
Metamorph |
| T9308 |
2139-2147 |
NNP |
denotes |
Software |
| T9307 |
2148-2152 |
NN |
denotes |
v6.2 |
| T9309 |
2153-2154 |
-LRB- |
denotes |
( |
| T9311 |
2154-2163 |
NNP |
denotes |
Universal |
| T9310 |
2164-2171 |
NNP |
denotes |
Imaging |
| T9312 |
2171-2173 |
, |
denotes |
, |
| T9313 |
2173-2181 |
NNP |
denotes |
Glendale |
| T9314 |
2181-2183 |
, |
denotes |
, |
| T9315 |
2183-2192 |
NNP |
denotes |
Wisconsin |
| T9316 |
2192-2194 |
, |
denotes |
, |
| T9317 |
2194-2200 |
NNP |
denotes |
United |
| T9318 |
2201-2207 |
NNP |
denotes |
States |
| T9319 |
2207-2208 |
-RRB- |
denotes |
) |
| T9320 |
2208-2209 |
. |
denotes |
. |
| T9321 |
2209-2398 |
sentence |
denotes |
Whenever applicable, serial optical Z-stacks (20–30 focal planes at 100-nm intervals) were captured and computationally processed by 3-D blind deconvolution methods with the same software. |
| T9322 |
2210-2218 |
RB |
denotes |
Whenever |
| T9323 |
2219-2229 |
JJ |
denotes |
applicable |
| T9325 |
2229-2231 |
, |
denotes |
, |
| T9326 |
2231-2237 |
JJ |
denotes |
serial |
| T9328 |
2238-2245 |
JJ |
denotes |
optical |
| T9329 |
2246-2247 |
NN |
denotes |
Z |
| T9330 |
2247-2248 |
HYPH |
denotes |
- |
| T9327 |
2248-2254 |
NNS |
denotes |
stacks |
| T9331 |
2255-2256 |
-LRB- |
denotes |
( |
| T9332 |
2256-2258 |
CD |
denotes |
20 |
| T9334 |
2258-2259 |
SYM |
denotes |
– |
| T9333 |
2259-2261 |
CD |
denotes |
30 |
| T9336 |
2262-2267 |
JJ |
denotes |
focal |
| T9335 |
2268-2274 |
NNS |
denotes |
planes |
| T9337 |
2275-2277 |
IN |
denotes |
at |
| T9338 |
2278-2281 |
CD |
denotes |
100 |
| T9340 |
2281-2282 |
HYPH |
denotes |
- |
| T9339 |
2282-2284 |
NN |
denotes |
nm |
| T9341 |
2285-2294 |
NNS |
denotes |
intervals |
| T9342 |
2294-2295 |
-RRB- |
denotes |
) |
| T9343 |
2296-2300 |
VBD |
denotes |
were |
| T9324 |
2301-2309 |
VBN |
denotes |
captured |
| T9344 |
2310-2313 |
CC |
denotes |
and |
| T9345 |
2314-2329 |
RB |
denotes |
computationally |
| T9346 |
2330-2339 |
VBN |
denotes |
processed |
| T9347 |
2340-2342 |
IN |
denotes |
by |
| T9348 |
2343-2344 |
CD |
denotes |
3 |
| T9350 |
2344-2345 |
HYPH |
denotes |
- |
| T9349 |
2345-2346 |
NN |
denotes |
D |
| T9352 |
2347-2352 |
JJ |
denotes |
blind |
| T9353 |
2353-2366 |
NN |
denotes |
deconvolution |
| T9351 |
2367-2374 |
NNS |
denotes |
methods |
| T9354 |
2375-2379 |
IN |
denotes |
with |
| T9355 |
2380-2383 |
DT |
denotes |
the |
| T9357 |
2384-2388 |
JJ |
denotes |
same |
| T9356 |
2389-2397 |
NN |
denotes |
software |
| T9358 |
2397-2398 |
. |
denotes |
. |
| R5837 |
T8888 |
T8889 |
compound |
Retina,dissections |
| R5838 |
T8889 |
T8890 |
nsubjpass |
dissections,carried |
| R5839 |
T8891 |
T8889 |
punct |
", ",dissections |
| R5840 |
T8892 |
T8893 |
amod |
radial,cryosections |
| R5841 |
T8893 |
T8889 |
conj |
cryosections,dissections |
| R5842 |
T8894 |
T8895 |
punct |
(,μm |
| R5843 |
T8895 |
T8893 |
parataxis |
μm,cryosections |
| R5844 |
T8896 |
T8897 |
punct |
~,6 |
| R5845 |
T8897 |
T8895 |
nummod |
6,μm |
| R5846 |
T8898 |
T8895 |
punct |
),μm |
| R5847 |
T8899 |
T8893 |
punct |
", ",cryosections |
| R5848 |
T8900 |
T8893 |
cc |
and,cryosections |
| R5849 |
T8901 |
T8902 |
compound |
immunohistochemistry,procedures |
| R5850 |
T8902 |
T8893 |
conj |
procedures,cryosections |
| R5851 |
T8903 |
T8890 |
auxpass |
were,carried |
| R5852 |
T8904 |
T8890 |
prt |
out,carried |
| R5853 |
T8905 |
T8906 |
mark |
as,described |
| R5854 |
T8906 |
T8890 |
advcl |
described,carried |
| R5855 |
T8907 |
T8906 |
advmod |
elsewhere,described |
| R5856 |
T8908 |
T8909 |
punct |
[,19 |
| R5857 |
T8909 |
T8906 |
parataxis |
19,described |
| R5858 |
T8910 |
T8909 |
punct |
],19 |
| R5859 |
T8911 |
T8890 |
punct |
.,carried |
| R5860 |
T8913 |
T8914 |
nsubjpass |
Brains,fixed |
| R5861 |
T8915 |
T8913 |
prep |
from,Brains |
| R5862 |
T8916 |
T8917 |
quantmod |
3,5 |
| R5863 |
T8917 |
T8920 |
nummod |
5,mo |
| R5864 |
T8918 |
T8917 |
punct |
-,5 |
| R5865 |
T8919 |
T8917 |
quantmod |
to,5 |
| R5866 |
T8920 |
T8922 |
npadvmod |
mo,old |
| R5867 |
T8921 |
T8920 |
punct |
-,mo |
| R5868 |
T8922 |
T8924 |
amod |
old,mice |
| R5869 |
T8923 |
T8922 |
punct |
-,old |
| R5870 |
T8924 |
T8915 |
pobj |
mice,from |
| R5871 |
T8925 |
T8924 |
nmod |
C57Bl,mice |
| R5872 |
T8926 |
T8925 |
punct |
/,C57Bl |
| R5873 |
T8927 |
T8925 |
nummod |
6,C57Bl |
| R5874 |
T8928 |
T8914 |
auxpass |
were,fixed |
| R5875 |
T8929 |
T8914 |
advmod |
overnight,fixed |
| R5876 |
T8930 |
T8914 |
prep |
in,fixed |
| R5877 |
T8931 |
T8932 |
nummod |
2,% |
| R5878 |
T8932 |
T8933 |
compound |
%,paraformaldehyde |
| R5879 |
T8933 |
T8930 |
pobj |
paraformaldehyde,in |
| R5880 |
T8934 |
T8914 |
punct |
", ",fixed |
| R5881 |
T8935 |
T8914 |
conj |
infused,fixed |
| R5882 |
T8936 |
T8935 |
prep |
with,infused |
| R5883 |
T8937 |
T8938 |
nummod |
30,% |
| R5884 |
T8938 |
T8939 |
compound |
%,sucrose |
| R5885 |
T8939 |
T8936 |
pobj |
sucrose,with |
| R5886 |
T8940 |
T8935 |
punct |
", ",infused |
| R5887 |
T8941 |
T8935 |
cc |
and,infused |
| R5888 |
T8942 |
T8935 |
conj |
processed,infused |
| R5889 |
T8943 |
T8942 |
prep |
for,processed |
| R5890 |
T8944 |
T8943 |
pobj |
cryosectioning,for |
| R5891 |
T8945 |
T8914 |
punct |
.,fixed |
| R5892 |
T8947 |
T8948 |
amod |
Primary,neurons |
| R5893 |
T8948 |
T8950 |
nsubjpass |
neurons,prepared |
| R5894 |
T8949 |
T8948 |
compound |
brain,neurons |
| R5895 |
T8951 |
T8948 |
cc |
and,neurons |
| R5896 |
T8952 |
T8953 |
amod |
glial,cells |
| R5897 |
T8953 |
T8948 |
conj |
cells,neurons |
| R5898 |
T8954 |
T8950 |
auxpass |
were,prepared |
| R5899 |
T8955 |
T8950 |
prep |
from,prepared |
| R5900 |
T8956 |
T8957 |
det |
the,cortex |
| R5901 |
T8957 |
T8955 |
pobj |
cortex,from |
| R5902 |
T8958 |
T8957 |
amod |
cerebral,cortex |
| R5903 |
T8959 |
T8950 |
punct |
.,prepared |
| R5904 |
T8961 |
T8962 |
nsubjpass |
This,macerated |
| R5905 |
T8963 |
T8962 |
auxpass |
was,macerated |
| R5906 |
T8964 |
T8962 |
prep |
in,macerated |
| R5907 |
T8965 |
T8966 |
det |
the,solution |
| R5908 |
T8966 |
T8964 |
pobj |
solution,in |
| R5909 |
T8967 |
T8968 |
poss |
Hank,Salt |
| R5910 |
T8968 |
T8966 |
compound |
Salt,solution |
| R5911 |
T8969 |
T8967 |
case |
's,Hank |
| R5912 |
T8970 |
T8968 |
compound |
Balanced,Salt |
| R5913 |
T8971 |
T8962 |
cc |
and,macerated |
| R5914 |
T8972 |
T8962 |
conj |
triturated,macerated |
| R5915 |
T8973 |
T8962 |
punct |
.,macerated |
| R5916 |
T8975 |
T8976 |
amod |
Primary,cells |
| R5917 |
T8976 |
T8977 |
nsubjpass |
cells,cultured |
| R5918 |
T8978 |
T8977 |
auxpass |
were,cultured |
| R5919 |
T8979 |
T8977 |
prep |
in,cultured |
| R5920 |
T8980 |
T8979 |
pobj |
DMEM,in |
| R5921 |
T8981 |
T8982 |
punct |
(,GIBCO |
| R5922 |
T8982 |
T8980 |
parataxis |
GIBCO,DMEM |
| R5923 |
T8983 |
T8982 |
punct |
", ",GIBCO |
| R5924 |
T8984 |
T8985 |
compound |
San,Diego |
| R5925 |
T8985 |
T8982 |
npadvmod |
Diego,GIBCO |
| R5926 |
T8986 |
T8982 |
punct |
", ",GIBCO |
| R5927 |
T8987 |
T8982 |
npadvmod |
California,GIBCO |
| R5928 |
T8988 |
T8982 |
punct |
", ",GIBCO |
| R5929 |
T8989 |
T8990 |
compound |
United,States |
| R5930 |
T8990 |
T8982 |
npadvmod |
States,GIBCO |
| R5931 |
T8991 |
T8982 |
punct |
),GIBCO |
| R5932 |
T8992 |
T8980 |
cc |
and,DMEM |
| R5933 |
T8993 |
T8994 |
npadvmod |
collagen,coated |
| R5934 |
T8994 |
T8996 |
amod |
coated,dishes |
| R5935 |
T8995 |
T8994 |
punct |
-,coated |
| R5936 |
T8996 |
T8980 |
conj |
dishes,DMEM |
| R5937 |
T8997 |
T8998 |
nummod |
35,mm |
| R5938 |
T8998 |
T8996 |
compound |
mm,dishes |
| R5939 |
T8999 |
T8998 |
punct |
-,mm |
| R5940 |
T9000 |
T9001 |
compound |
glass,bottom |
| R5941 |
T9001 |
T8996 |
compound |
bottom,dishes |
| R5942 |
T9002 |
T8996 |
compound |
culture,dishes |
| R5943 |
T9003 |
T9004 |
punct |
(,Corporation |
| R5944 |
T9004 |
T8996 |
parataxis |
Corporation,dishes |
| R5945 |
T9005 |
T9004 |
compound |
MatTek,Corporation |
| R5946 |
T9006 |
T9004 |
punct |
", ",Corporation |
| R5947 |
T9007 |
T9004 |
npadvmod |
Ashland,Corporation |
| R5948 |
T9008 |
T9004 |
punct |
", ",Corporation |
| R5949 |
T9009 |
T9004 |
npadvmod |
Maine,Corporation |
| R5950 |
T9010 |
T9004 |
punct |
),Corporation |
| R5951 |
T9011 |
T8977 |
prep |
at,cultured |
| R5952 |
T9012 |
T9013 |
nummod |
5,% |
| R5953 |
T9013 |
T9014 |
compound |
%,CO2 |
| R5954 |
T9014 |
T9011 |
pobj |
CO2,at |
| R5955 |
T9015 |
T9014 |
punct |
/,CO2 |
| R5956 |
T9016 |
T9017 |
nummod |
37,°C |
| R5957 |
T9017 |
T9014 |
appos |
°C,CO2 |
| R5958 |
T9018 |
T8977 |
prep |
for,cultured |
| R5959 |
T9019 |
T9020 |
punct |
~,2 |
| R5960 |
T9020 |
T9021 |
nummod |
2,d |
| R5961 |
T9021 |
T9018 |
pobj |
d,for |
| R5962 |
T9022 |
T8977 |
cc |
and,cultured |
| R5963 |
T9023 |
T8977 |
conj |
processed,cultured |
| R5964 |
T9024 |
T9023 |
prep |
for,processed |
| R5965 |
T9025 |
T9024 |
pobj |
immunocytochemistry,for |
| R5966 |
T9026 |
T8977 |
punct |
.,cultured |
| R5967 |
T9028 |
T9029 |
amod |
Primary,antibodies |
| R5968 |
T9029 |
T9030 |
nsubjpass |
antibodies,used |
| R5969 |
T9031 |
T9030 |
auxpass |
were,used |
| R5970 |
T9032 |
T9030 |
prep |
at,used |
| R5971 |
T9033 |
T9032 |
pobj |
concentrations,at |
| R5972 |
T9034 |
T9035 |
punct |
~,10 |
| R5973 |
T9035 |
T9038 |
nummod |
10,μg |
| R5974 |
T9036 |
T9035 |
quantmod |
2.5,10 |
| R5975 |
T9037 |
T9035 |
punct |
–,10 |
| R5976 |
T9038 |
T9033 |
npadvmod |
μg,concentrations |
| R5977 |
T9039 |
T9040 |
punct |
/,ml |
| R5978 |
T9040 |
T9038 |
prep |
ml,μg |
| R5979 |
T9041 |
T9030 |
punct |
.,used |
| R5980 |
T9043 |
T9044 |
npadvmod |
Alexa,conjugated |
| R5981 |
T9044 |
T9051 |
amod |
conjugated,antibodies |
| R5982 |
T9045 |
T9043 |
nummod |
488,Alexa |
| R5983 |
T9046 |
T9043 |
punct |
-,Alexa |
| R5984 |
T9047 |
T9043 |
cc |
and,Alexa |
| R5985 |
T9048 |
T9043 |
conj |
Alexa,Alexa |
| R5986 |
T9049 |
T9048 |
amod |
594,Alexa |
| R5987 |
T9050 |
T9044 |
punct |
-,conjugated |
| R5988 |
T9051 |
T9053 |
nsubjpass |
antibodies,used |
| R5989 |
T9052 |
T9051 |
amod |
secondary,antibodies |
| R5990 |
T9054 |
T9055 |
punct |
(,μg |
| R5991 |
T9055 |
T9051 |
parataxis |
μg,antibodies |
| R5992 |
T9056 |
T9055 |
nummod |
2.5,μg |
| R5993 |
T9057 |
T9058 |
punct |
/,ml |
| R5994 |
T9058 |
T9055 |
prep |
ml,μg |
| R5995 |
T9059 |
T9055 |
punct |
),μg |
| R5996 |
T9060 |
T9061 |
punct |
(,Probes |
| R5997 |
T9061 |
T9051 |
parataxis |
Probes,antibodies |
| R5998 |
T9062 |
T9061 |
compound |
Molecular,Probes |
| R5999 |
T9063 |
T9061 |
punct |
),Probes |
| R6000 |
T9064 |
T9053 |
auxpass |
were,used |
| R6001 |
T9065 |
T9053 |
prep |
for,used |
| R6002 |
T9066 |
T9065 |
pobj |
visualization,for |
| R6003 |
T9067 |
T9066 |
prep |
of,visualization |
| R6004 |
T9068 |
T9067 |
pobj |
proteins,of |
| R6005 |
T9069 |
T9053 |
punct |
.,used |
| R6006 |
T9071 |
T9072 |
nsubjpass |
Crossover,found |
| R6007 |
T9073 |
T9071 |
prep |
of,Crossover |
| R6008 |
T9074 |
T9075 |
amod |
fluorescent,probes |
| R6009 |
T9075 |
T9073 |
pobj |
probes,of |
| R6010 |
T9076 |
T9075 |
punct |
", ",probes |
| R6011 |
T9077 |
T9075 |
conj |
background,probes |
| R6012 |
T9078 |
T9077 |
punct |
", ",background |
| R6013 |
T9079 |
T9077 |
cc |
and,background |
| R6014 |
T9080 |
T9077 |
conj |
autofluorescence,background |
| R6015 |
T9081 |
T9072 |
auxpass |
were,found |
| R6016 |
T9082 |
T9083 |
aux |
to,be |
| R6017 |
T9083 |
T9072 |
xcomp |
be,found |
| R6018 |
T9084 |
T9083 |
acomp |
negligible,be |
| R6019 |
T9085 |
T9072 |
punct |
.,found |
| R6020 |
T9087 |
T9088 |
nsubjpass |
Visualization,carried |
| R6021 |
T9089 |
T9087 |
prep |
of,Visualization |
| R6022 |
T9090 |
T9089 |
pobj |
specimens,of |
| R6023 |
T9091 |
T9087 |
cc |
and,Visualization |
| R6024 |
T9092 |
T9087 |
conj |
localization,Visualization |
| R6025 |
T9093 |
T9092 |
prep |
of,localization |
| R6026 |
T9094 |
T9093 |
pobj |
proteins,of |
| R6027 |
T9095 |
T9088 |
auxpass |
were,carried |
| R6028 |
T9096 |
T9088 |
prt |
out,carried |
| R6029 |
T9097 |
T9088 |
prep |
by,carried |
| R6030 |
T9098 |
T9099 |
amod |
wide,field |
| R6031 |
T9099 |
T9101 |
compound |
field,microscopy |
| R6032 |
T9100 |
T9099 |
punct |
-,field |
| R6033 |
T9101 |
T9097 |
pobj |
microscopy,by |
| R6034 |
T9102 |
T9101 |
compound |
epifluorescence,microscopy |
| R6035 |
T9103 |
T9088 |
prep |
on,carried |
| R6036 |
T9104 |
T9105 |
nmod |
Nikon,upright |
| R6037 |
T9105 |
T9103 |
pobj |
upright,on |
| R6038 |
T9106 |
T9107 |
punct |
(,Tokyo |
| R6039 |
T9107 |
T9104 |
parataxis |
Tokyo,Nikon |
| R6040 |
T9108 |
T9107 |
punct |
", ",Tokyo |
| R6041 |
T9109 |
T9107 |
npadvmod |
Japan,Tokyo |
| R6042 |
T9110 |
T9107 |
punct |
),Tokyo |
| R6043 |
T9111 |
T9105 |
compound |
E600,upright |
| R6044 |
T9112 |
T9105 |
cc |
and,upright |
| R6045 |
T9113 |
T9114 |
nmod |
TE2000U,microscopes |
| R6046 |
T9114 |
T9105 |
conj |
microscopes,upright |
| R6047 |
T9115 |
T9113 |
amod |
inverted,TE2000U |
| R6048 |
T9116 |
T9114 |
compound |
research,microscopes |
| R6049 |
T9117 |
T9105 |
acl |
equipped,upright |
| R6050 |
T9118 |
T9117 |
prep |
with,equipped |
| R6051 |
T9119 |
T9120 |
amod |
similar,objectives |
| R6052 |
T9120 |
T9118 |
pobj |
objectives,with |
| R6053 |
T9121 |
T9120 |
compound |
Apochromat,objectives |
| R6054 |
T9122 |
T9088 |
punct |
.,carried |
| R6055 |
T9124 |
T9125 |
nsubjpass |
Images,acquired |
| R6056 |
T9126 |
T9124 |
prep |
with,Images |
| R6057 |
T9127 |
T9128 |
det |
the,E600 |
| R6058 |
T9128 |
T9126 |
pobj |
E600,with |
| R6059 |
T9129 |
T9128 |
compound |
Nikon,E600 |
| R6060 |
T9130 |
T9125 |
auxpass |
were,acquired |
| R6061 |
T9131 |
T9125 |
prep |
with,acquired |
| R6062 |
T9132 |
T9133 |
det |
a,camera |
| R6063 |
T9133 |
T9131 |
pobj |
camera,with |
| R6064 |
T9134 |
T9135 |
nmod |
SPOT,RT |
| R6065 |
T9135 |
T9133 |
nmod |
RT,camera |
| R6066 |
T9136 |
T9135 |
punct |
-,RT |
| R6067 |
T9137 |
T9133 |
amod |
digital,camera |
| R6068 |
T9138 |
T9133 |
acl |
coupled,camera |
| R6069 |
T9139 |
T9138 |
prep |
to,coupled |
| R6070 |
T9140 |
T9141 |
det |
the,microscope |
| R6071 |
T9141 |
T9139 |
pobj |
microscope,to |
| R6072 |
T9142 |
T9138 |
cc |
and,coupled |
| R6073 |
T9143 |
T9138 |
conj |
driven,coupled |
| R6074 |
T9144 |
T9143 |
agent |
by,driven |
| R6075 |
T9145 |
T9146 |
compound |
SPOT,v4.0 |
| R6076 |
T9146 |
T9148 |
compound |
v4.0,software |
| R6077 |
T9147 |
T9146 |
compound |
Imaging,v4.0 |
| R6078 |
T9148 |
T9144 |
pobj |
software,by |
| R6079 |
T9149 |
T9150 |
punct |
(,Instruments |
| R6080 |
T9150 |
T9148 |
parataxis |
Instruments,software |
| R6081 |
T9151 |
T9150 |
compound |
Diagnostic,Instruments |
| R6082 |
T9152 |
T9150 |
punct |
", ",Instruments |
| R6083 |
T9153 |
T9154 |
compound |
Sterling,Heights |
| R6084 |
T9154 |
T9150 |
npadvmod |
Heights,Instruments |
| R6085 |
T9155 |
T9150 |
punct |
", ",Instruments |
| R6086 |
T9156 |
T9150 |
npadvmod |
Michigan,Instruments |
| R6087 |
T9157 |
T9150 |
punct |
", ",Instruments |
| R6088 |
T9158 |
T9159 |
compound |
United,States |
| R6089 |
T9159 |
T9150 |
npadvmod |
States,Instruments |
| R6090 |
T9160 |
T9150 |
punct |
),Instruments |
| R6091 |
T9161 |
T9125 |
punct |
.,acquired |
| R6092 |
T9163 |
T9164 |
det |
All,images |
| R6093 |
T9164 |
T9165 |
nsubjpass |
images,captured |
| R6094 |
T9166 |
T9165 |
auxpass |
were,captured |
| R6095 |
T9167 |
T9165 |
prep |
at,captured |
| R6096 |
T9168 |
T9169 |
compound |
nonsaturating,levels |
| R6097 |
T9169 |
T9167 |
pobj |
levels,at |
| R6098 |
T9170 |
T9169 |
compound |
integration,levels |
| R6099 |
T9171 |
T9169 |
punct |
", ",levels |
| R6100 |
T9172 |
T9173 |
nummod |
12,bit |
| R6101 |
T9173 |
T9175 |
nmod |
bit,white |
| R6102 |
T9174 |
T9173 |
punct |
-,bit |
| R6103 |
T9175 |
T9169 |
appos |
white,levels |
| R6104 |
T9176 |
T9175 |
nmod |
mono,white |
| R6105 |
T9177 |
T9175 |
amod |
black,white |
| R6106 |
T9178 |
T9175 |
punct |
/,white |
| R6107 |
T9179 |
T9165 |
punct |
", ",captured |
| R6108 |
T9180 |
T9165 |
cc |
and,captured |
| R6109 |
T9181 |
T9182 |
advmod |
then,pseudo-colored |
| R6110 |
T9182 |
T9165 |
conj |
pseudo-colored,captured |
| R6111 |
T9183 |
T9165 |
punct |
.,captured |
| R6112 |
T9185 |
T9186 |
compound |
Protein,colocalization |
| R6113 |
T9186 |
T9187 |
compound |
colocalization,analysis |
| R6114 |
T9187 |
T9188 |
nsubjpass |
analysis,carried |
| R6115 |
T9189 |
T9188 |
auxpass |
was,carried |
| R6116 |
T9190 |
T9188 |
prt |
out,carried |
| R6117 |
T9191 |
T9188 |
prep |
on,carried |
| R6118 |
T9192 |
T9193 |
det |
the,microscope |
| R6119 |
T9193 |
T9191 |
pobj |
microscope,on |
| R6120 |
T9194 |
T9195 |
compound |
Nikon,TE2000U |
| R6121 |
T9195 |
T9193 |
compound |
TE2000U,microscope |
| R6122 |
T9196 |
T9193 |
acl |
equipped,microscope |
| R6123 |
T9197 |
T9196 |
prep |
with,equipped |
| R6124 |
T9198 |
T9199 |
amod |
appropriate,wheels |
| R6125 |
T9199 |
T9197 |
pobj |
wheels,with |
| R6126 |
T9200 |
T9199 |
nmod |
excitation,wheels |
| R6127 |
T9201 |
T9200 |
cc |
and,excitation |
| R6128 |
T9202 |
T9200 |
conj |
emission,excitation |
| R6129 |
T9203 |
T9199 |
compound |
filter,wheels |
| R6130 |
T9204 |
T9199 |
punct |
", ",wheels |
| R6131 |
T9205 |
T9206 |
compound |
cube,filters |
| R6132 |
T9206 |
T9199 |
appos |
filters,wheels |
| R6133 |
T9207 |
T9199 |
punct |
", ",wheels |
| R6134 |
T9208 |
T9209 |
nummod |
100,W |
| R6135 |
T9209 |
T9210 |
compound |
W,source |
| R6136 |
T9210 |
T9199 |
appos |
source,wheels |
| R6137 |
T9211 |
T9210 |
compound |
mercury,source |
| R6138 |
T9212 |
T9210 |
compound |
light,source |
| R6139 |
T9213 |
T9199 |
punct |
", ",wheels |
| R6140 |
T9214 |
T9215 |
nmod |
Nomarski,DIC |
| R6141 |
T9215 |
T9217 |
nmod |
DIC,optics |
| R6142 |
T9216 |
T9215 |
punct |
/,DIC |
| R6143 |
T9217 |
T9199 |
appos |
optics,wheels |
| R6144 |
T9218 |
T9215 |
punct |
", ",DIC |
| R6145 |
T9219 |
T9215 |
cc |
and,DIC |
| R6146 |
T9220 |
T9221 |
compound |
Plan,Apochromat |
| R6147 |
T9221 |
T9215 |
conj |
Apochromat,DIC |
| R6148 |
T9222 |
T9217 |
punct |
(,optics |
| R6149 |
T9223 |
T9224 |
nummod |
100,objectives |
| R6150 |
T9224 |
T9217 |
appos |
objectives,optics |
| R6151 |
T9225 |
T9223 |
punct |
×,100 |
| R6152 |
T9226 |
T9223 |
punct |
", ",100 |
| R6153 |
T9227 |
T9223 |
conj |
60,100 |
| R6154 |
T9228 |
T9227 |
punct |
×,60 |
| R6155 |
T9229 |
T9227 |
punct |
", ",60 |
| R6156 |
T9230 |
T9227 |
cc |
and,60 |
| R6157 |
T9231 |
T9227 |
conj |
40,60 |
| R6158 |
T9232 |
T9231 |
punct |
×,40 |
| R6159 |
T9233 |
T9224 |
compound |
oil,objectives |
| R6160 |
T9234 |
T9224 |
prep |
with,objectives |
| R6161 |
T9235 |
T9234 |
pobj |
NA,with |
| R6162 |
T9236 |
T9235 |
prep |
of,NA |
| R6163 |
T9237 |
T9236 |
pobj |
1.4,of |
| R6164 |
T9238 |
T9224 |
punct |
", ",objectives |
| R6165 |
T9239 |
T9240 |
nummod |
40,LWD |
| R6166 |
T9240 |
T9242 |
nmod |
LWD,objectives |
| R6167 |
T9241 |
T9239 |
punct |
×,40 |
| R6168 |
T9242 |
T9224 |
conj |
objectives,objectives |
| R6169 |
T9243 |
T9240 |
punct |
", ",LWD |
| R6170 |
T9244 |
T9240 |
cc |
and,LWD |
| R6171 |
T9245 |
T9246 |
nummod |
20,LWD |
| R6172 |
T9246 |
T9240 |
conj |
LWD,LWD |
| R6173 |
T9247 |
T9245 |
punct |
×,20 |
| R6174 |
T9248 |
T9242 |
cc |
and,objectives |
| R6175 |
T9249 |
T9250 |
amod |
encoded,Stage |
| R6176 |
T9250 |
T9242 |
conj |
Stage,objectives |
| R6177 |
T9251 |
T9250 |
amod |
motorized,Stage |
| R6178 |
T9252 |
T9250 |
compound |
Z,Stage |
| R6179 |
T9253 |
T9250 |
punct |
-,Stage |
| R6180 |
T9254 |
T9255 |
punct |
(,Scientific |
| R6181 |
T9255 |
T9250 |
parataxis |
Scientific,Stage |
| R6182 |
T9256 |
T9255 |
compound |
Prior,Scientific |
| R6183 |
T9257 |
T9255 |
punct |
", ",Scientific |
| R6184 |
T9258 |
T9255 |
npadvmod |
Rockland,Scientific |
| R6185 |
T9259 |
T9255 |
punct |
", ",Scientific |
| R6186 |
T9260 |
T9255 |
npadvmod |
Maryland,Scientific |
| R6187 |
T9261 |
T9255 |
punct |
", ",Scientific |
| R6188 |
T9262 |
T9263 |
compound |
United,States |
| R6189 |
T9263 |
T9255 |
npadvmod |
States,Scientific |
| R6190 |
T9264 |
T9255 |
punct |
),Scientific |
| R6191 |
T9265 |
T9188 |
punct |
.,carried |
| R6192 |
T9267 |
T9268 |
nsubjpass |
Images,obtained |
| R6193 |
T9269 |
T9267 |
prep |
with,Images |
| R6194 |
T9270 |
T9271 |
det |
the,microscope |
| R6195 |
T9271 |
T9269 |
pobj |
microscope,with |
| R6196 |
T9272 |
T9271 |
amod |
inverted,microscope |
| R6197 |
T9273 |
T9274 |
compound |
Nikon,TE2000U |
| R6198 |
T9274 |
T9271 |
compound |
TE2000U,microscope |
| R6199 |
T9275 |
T9268 |
auxpass |
were,obtained |
| R6200 |
T9276 |
T9268 |
advcl |
using,obtained |
| R6201 |
T9277 |
T9278 |
det |
a,camera |
| R6202 |
T9278 |
T9276 |
dobj |
camera,using |
| R6203 |
T9279 |
T9278 |
compound |
CCD,camera |
| R6204 |
T9280 |
T9278 |
punct |
(,camera |
| R6205 |
T9281 |
T9282 |
compound |
CoolSNAP,HQ |
| R6206 |
T9282 |
T9278 |
appos |
HQ,camera |
| R6207 |
T9283 |
T9284 |
punct |
;,Scientific |
| R6208 |
T9284 |
T9278 |
parataxis |
Scientific,camera |
| R6209 |
T9285 |
T9284 |
compound |
Roper,Scientific |
| R6210 |
T9286 |
T9284 |
punct |
", ",Scientific |
| R6211 |
T9287 |
T9284 |
npadvmod |
Trenton,Scientific |
| R6212 |
T9288 |
T9284 |
punct |
", ",Scientific |
| R6213 |
T9289 |
T9290 |
compound |
New,Jersey |
| R6214 |
T9290 |
T9284 |
npadvmod |
Jersey,Scientific |
| R6215 |
T9291 |
T9284 |
punct |
", ",Scientific |
| R6216 |
T9292 |
T9293 |
compound |
United,States |
| R6217 |
T9293 |
T9284 |
npadvmod |
States,Scientific |
| R6218 |
T9294 |
T9284 |
punct |
),Scientific |
| R6219 |
T9295 |
T9268 |
punct |
.,obtained |
| R6220 |
T9297 |
T9298 |
nsubjpass |
Images,analyzed |
| R6221 |
T9299 |
T9297 |
acl |
acquired,Images |
| R6222 |
T9300 |
T9299 |
prep |
under,acquired |
| R6223 |
T9301 |
T9302 |
amod |
identical,parameters |
| R6224 |
T9302 |
T9300 |
pobj |
parameters,under |
| R6225 |
T9303 |
T9302 |
compound |
acquisition,parameters |
| R6226 |
T9304 |
T9298 |
auxpass |
were,analyzed |
| R6227 |
T9305 |
T9298 |
advcl |
using,analyzed |
| R6228 |
T9306 |
T9307 |
compound |
Metamorph,v6.2 |
| R6229 |
T9307 |
T9305 |
dobj |
v6.2,using |
| R6230 |
T9308 |
T9307 |
compound |
Software,v6.2 |
| R6231 |
T9309 |
T9310 |
punct |
(,Imaging |
| R6232 |
T9310 |
T9307 |
parataxis |
Imaging,v6.2 |
| R6233 |
T9311 |
T9310 |
compound |
Universal,Imaging |
| R6234 |
T9312 |
T9310 |
punct |
", ",Imaging |
| R6235 |
T9313 |
T9310 |
npadvmod |
Glendale,Imaging |
| R6236 |
T9314 |
T9310 |
punct |
", ",Imaging |
| R6237 |
T9315 |
T9310 |
npadvmod |
Wisconsin,Imaging |
| R6238 |
T9316 |
T9310 |
punct |
", ",Imaging |
| R6239 |
T9317 |
T9318 |
compound |
United,States |
| R6240 |
T9318 |
T9310 |
npadvmod |
States,Imaging |
| R6241 |
T9319 |
T9310 |
punct |
),Imaging |
| R6242 |
T9320 |
T9298 |
punct |
.,analyzed |
| R6243 |
T9322 |
T9323 |
advmod |
Whenever,applicable |
| R6244 |
T9323 |
T9324 |
advcl |
applicable,captured |
| R6245 |
T9325 |
T9324 |
punct |
", ",captured |
| R6246 |
T9326 |
T9327 |
amod |
serial,stacks |
| R6247 |
T9327 |
T9324 |
nsubjpass |
stacks,captured |
| R6248 |
T9328 |
T9327 |
amod |
optical,stacks |
| R6249 |
T9329 |
T9327 |
compound |
Z,stacks |
| R6250 |
T9330 |
T9327 |
punct |
-,stacks |
| R6251 |
T9331 |
T9327 |
punct |
(,stacks |
| R6252 |
T9332 |
T9333 |
quantmod |
20,30 |
| R6253 |
T9333 |
T9335 |
nummod |
30,planes |
| R6254 |
T9334 |
T9333 |
punct |
–,30 |
| R6255 |
T9335 |
T9327 |
appos |
planes,stacks |
| R6256 |
T9336 |
T9335 |
amod |
focal,planes |
| R6257 |
T9337 |
T9335 |
prep |
at,planes |
| R6258 |
T9338 |
T9339 |
nummod |
100,nm |
| R6259 |
T9339 |
T9341 |
compound |
nm,intervals |
| R6260 |
T9340 |
T9339 |
punct |
-,nm |
| R6261 |
T9341 |
T9337 |
pobj |
intervals,at |
| R6262 |
T9342 |
T9324 |
punct |
),captured |
| R6263 |
T9343 |
T9324 |
auxpass |
were,captured |
| R6264 |
T9344 |
T9324 |
cc |
and,captured |
| R6265 |
T9345 |
T9346 |
advmod |
computationally,processed |
| R6266 |
T9346 |
T9324 |
conj |
processed,captured |
| R6267 |
T9347 |
T9346 |
prep |
by,processed |
| R6268 |
T9348 |
T9349 |
nummod |
3,D |
| R6269 |
T9349 |
T9351 |
nmod |
D,methods |
| R6270 |
T9350 |
T9349 |
punct |
-,D |
| R6271 |
T9351 |
T9347 |
pobj |
methods,by |
| R6272 |
T9352 |
T9351 |
amod |
blind,methods |
| R6273 |
T9353 |
T9351 |
compound |
deconvolution,methods |
| R6274 |
T9354 |
T9346 |
prep |
with,processed |
| R6275 |
T9355 |
T9356 |
det |
the,software |
| R6276 |
T9356 |
T9354 |
pobj |
software,with |
| R6277 |
T9357 |
T9356 |
amod |
same,software |
| R6278 |
T9358 |
T9324 |
punct |
.,captured |
