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PMC:1626108 / 42944-43738 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T8257 0-3 NN denotes GST
T8259 4-8 VB denotes pull
T8260 8-9 HYPH denotes -
T8261 9-13 RP denotes down
T8262 14-17 CC denotes and
T8263 18-37 NN denotes immunoprecipitation
T8258 38-44 NNS denotes assays
T8264 44-45 . denotes .
T8265 45-178 sentence denotes CHAPS-solubilized retinal extracts, expression, and purification of GST-fused constructs were prepared as previously described [65].
T8266 46-51 NN denotes CHAPS
T8268 51-52 HYPH denotes -
T8267 52-63 VBN denotes solubilized
T8270 64-71 JJ denotes retinal
T8269 72-80 NNS denotes extracts
T8272 80-82 , denotes ,
T8273 82-92 NN denotes expression
T8274 92-94 , denotes ,
T8275 94-97 CC denotes and
T8276 98-110 NN denotes purification
T8277 111-113 IN denotes of
T8278 114-117 NN denotes GST
T8280 117-118 HYPH denotes -
T8279 118-123 VBN denotes fused
T8281 124-134 NNS denotes constructs
T8282 135-139 VBD denotes were
T8271 140-148 VBN denotes prepared
T8283 149-151 IN denotes as
T8285 152-162 RB denotes previously
T8284 163-172 VBN denotes described
T8286 173-174 -LRB- denotes [
T8287 174-176 CD denotes 65
T8288 176-177 -RRB- denotes ]
T8289 177-178 . denotes .
T8290 178-260 sentence denotes GST pull-down assays were carried out with 0.5–2.2 μM of GST-fused proteins [65].
T8291 179-182 NN denotes GST
T8293 183-187 VB denotes pull
T8294 187-188 HYPH denotes -
T8295 188-192 RP denotes down
T8292 193-199 NNS denotes assays
T8297 200-204 VBD denotes were
T8296 205-212 VBN denotes carried
T8298 213-216 RP denotes out
T8299 217-221 IN denotes with
T8300 222-225 CD denotes 0.5
T8302 225-226 SYM denotes –
T8301 226-229 CD denotes 2.2
T8303 230-232 NNS denotes μM
T8304 233-235 IN denotes of
T8305 236-239 NN denotes GST
T8307 239-240 HYPH denotes -
T8306 240-245 VBN denotes fused
T8308 246-254 NN denotes proteins
T8309 255-256 -LRB- denotes [
T8310 256-258 CD denotes 65
T8311 258-259 -RRB- denotes ]
T8312 259-260 . denotes .
T8313 260-381 sentence denotes Co-precipitates were resolved on SDS-PAGE and analyzed by Western blot with antibodies described in the Results section.
T8314 261-276 NNS denotes Co-precipitates
T8316 277-281 VBD denotes were
T8315 282-290 VBN denotes resolved
T8317 291-293 IN denotes on
T8318 294-297 NN denotes SDS
T8320 297-298 HYPH denotes -
T8319 298-302 NN denotes PAGE
T8321 303-306 CC denotes and
T8322 307-315 VBN denotes analyzed
T8323 316-318 IN denotes by
T8324 319-326 NNP denotes Western
T8325 327-331 NN denotes blot
T8326 332-336 IN denotes with
T8327 337-347 NNS denotes antibodies
T8328 348-357 VBN denotes described
T8329 358-360 IN denotes in
T8330 361-364 DT denotes the
T8332 365-372 NNS denotes Results
T8331 373-380 NN denotes section
T8333 380-381 . denotes .
T8334 381-553 sentence denotes Unfolded and partially denatured Cox11 were generated by incubating recombinant and native Cox11 overnight with 5.6 and 7 M guanidine hydrochloride and urea, respectively.
T8335 382-390 VBN denotes Unfolded
T8337 391-394 CC denotes and
T8338 395-404 RB denotes partially
T8339 405-414 VBN denotes denatured
T8336 415-420 NN denotes Cox11
T8341 421-425 VBD denotes were
T8340 426-435 VBN denotes generated
T8342 436-438 IN denotes by
T8343 439-449 VBG denotes incubating
T8344 450-461 JJ denotes recombinant
T8346 462-465 CC denotes and
T8347 466-472 JJ denotes native
T8345 473-478 NN denotes Cox11
T8348 479-488 RB denotes overnight
T8349 489-493 IN denotes with
T8350 494-497 CD denotes 5.6
T8352 498-501 CC denotes and
T8353 502-503 CD denotes 7
T8351 504-505 NNS denotes M
T8354 506-515 NN denotes guanidine
T8355 516-529 NN denotes hydrochloride
T8356 530-533 CC denotes and
T8357 534-538 NN denotes urea
T8358 538-540 , denotes ,
T8359 540-552 RB denotes respectively
T8360 552-553 . denotes .
T8361 553-644 sentence denotes The Cox11 conformers were then diluted ~20-fold in CHAPS-binding buffer containing GST-LD.
T8362 554-557 DT denotes The
T8364 558-563 NN denotes Cox11
T8363 564-574 NNS denotes conformers
T8366 575-579 VBD denotes were
T8367 580-584 RB denotes then
T8365 585-592 VBN denotes diluted
T8368 593-594 SYM denotes ~
T8369 594-601 RB denotes 20-fold
T8370 602-604 IN denotes in
T8371 605-610 NN denotes CHAPS
T8373 610-611 HYPH denotes -
T8372 611-618 NN denotes binding
T8374 619-625 NN denotes buffer
T8375 626-636 VBG denotes containing
T8376 637-640 NN denotes GST
T8378 640-641 HYPH denotes -
T8377 641-643 NN denotes LD
T8379 643-644 . denotes .
T8380 644-794 sentence denotes Immunoprecipitation assays with 5 μg of antibody and Western blots (~ 200–400 ng/ml of antibody) were performed exactly as described previously [13].
T8381 645-664 NN denotes Immunoprecipitation
T8382 665-671 NNS denotes assays
T8384 672-676 IN denotes with
T8385 677-678 CD denotes 5
T8386 679-681 NNS denotes μg
T8387 682-684 IN denotes of
T8388 685-693 NN denotes antibody
T8389 694-697 CC denotes and
T8390 698-705 NNP denotes Western
T8391 706-711 NNS denotes blots
T8392 712-713 -LRB- denotes (
T8394 713-714 SYM denotes ~
T8396 715-718 CD denotes 200
T8397 718-719 SYM denotes –
T8395 719-722 CD denotes 400
T8393 723-725 NN denotes ng
T8398 725-726 SYM denotes /
T8399 726-728 NN denotes ml
T8400 729-731 IN denotes of
T8401 732-740 NN denotes antibody
T8402 740-741 -RRB- denotes )
T8403 742-746 VBD denotes were
T8383 747-756 VBN denotes performed
T8404 757-764 RB denotes exactly
T8406 765-767 IN denotes as
T8405 768-777 VBN denotes described
T8407 778-788 RB denotes previously
T8408 789-790 -LRB- denotes [
T8409 790-792 CD denotes 13
T8410 792-793 -RRB- denotes ]
T8411 793-794 . denotes .
R5420 T8257 T8258 nmod GST,assays
R5421 T8259 T8258 amod pull,assays
R5422 T8260 T8259 punct -,pull
R5423 T8261 T8259 prt down,pull
R5424 T8262 T8259 cc and,pull
R5425 T8263 T8259 conj immunoprecipitation,pull
R5426 T8264 T8258 punct .,assays
R5427 T8266 T8267 npadvmod CHAPS,solubilized
R5428 T8267 T8269 amod solubilized,extracts
R5429 T8268 T8267 punct -,solubilized
R5430 T8269 T8271 nsubjpass extracts,prepared
R5431 T8270 T8269 amod retinal,extracts
R5432 T8272 T8269 punct ", ",extracts
R5433 T8273 T8269 conj expression,extracts
R5434 T8274 T8273 punct ", ",expression
R5435 T8275 T8273 cc and,expression
R5436 T8276 T8273 conj purification,expression
R5437 T8277 T8276 prep of,purification
R5438 T8278 T8279 npadvmod GST,fused
R5439 T8279 T8281 amod fused,constructs
R5440 T8280 T8279 punct -,fused
R5441 T8281 T8277 pobj constructs,of
R5442 T8282 T8271 auxpass were,prepared
R5443 T8283 T8284 mark as,described
R5444 T8284 T8271 advcl described,prepared
R5445 T8285 T8284 advmod previously,described
R5446 T8286 T8287 punct [,65
R5447 T8287 T8271 parataxis 65,prepared
R5448 T8288 T8287 punct ],65
R5449 T8289 T8271 punct .,prepared
R5450 T8291 T8292 nmod GST,assays
R5451 T8292 T8296 nsubjpass assays,carried
R5452 T8293 T8292 amod pull,assays
R5453 T8294 T8293 punct -,pull
R5454 T8295 T8293 prt down,pull
R5455 T8297 T8296 auxpass were,carried
R5456 T8298 T8296 prt out,carried
R5457 T8299 T8296 prep with,carried
R5458 T8300 T8301 quantmod 0.5,2.2
R5459 T8301 T8303 nummod 2.2,μM
R5460 T8302 T8301 punct –,2.2
R5461 T8303 T8299 pobj μM,with
R5462 T8304 T8303 prep of,μM
R5463 T8305 T8306 npadvmod GST,fused
R5464 T8306 T8308 amod fused,proteins
R5465 T8307 T8306 punct -,fused
R5466 T8308 T8304 pobj proteins,of
R5467 T8309 T8310 punct [,65
R5468 T8310 T8296 parataxis 65,carried
R5469 T8311 T8310 punct ],65
R5470 T8312 T8296 punct .,carried
R5471 T8314 T8315 nsubjpass Co-precipitates,resolved
R5472 T8316 T8315 auxpass were,resolved
R5473 T8317 T8315 prep on,resolved
R5474 T8318 T8319 compound SDS,PAGE
R5475 T8319 T8317 pobj PAGE,on
R5476 T8320 T8319 punct -,PAGE
R5477 T8321 T8315 cc and,resolved
R5478 T8322 T8315 conj analyzed,resolved
R5479 T8323 T8322 prep by,analyzed
R5480 T8324 T8325 compound Western,blot
R5481 T8325 T8323 pobj blot,by
R5482 T8326 T8322 prep with,analyzed
R5483 T8327 T8326 pobj antibodies,with
R5484 T8328 T8327 acl described,antibodies
R5485 T8329 T8328 prep in,described
R5486 T8330 T8331 det the,section
R5487 T8331 T8329 pobj section,in
R5488 T8332 T8331 compound Results,section
R5489 T8333 T8315 punct .,resolved
R5490 T8335 T8336 amod Unfolded,Cox11
R5491 T8336 T8340 nsubjpass Cox11,generated
R5492 T8337 T8335 cc and,Unfolded
R5493 T8338 T8339 advmod partially,denatured
R5494 T8339 T8335 conj denatured,Unfolded
R5495 T8341 T8340 auxpass were,generated
R5496 T8342 T8340 prep by,generated
R5497 T8343 T8342 pcomp incubating,by
R5498 T8344 T8345 amod recombinant,Cox11
R5499 T8345 T8343 dobj Cox11,incubating
R5500 T8346 T8344 cc and,recombinant
R5501 T8347 T8344 conj native,recombinant
R5502 T8348 T8340 advmod overnight,generated
R5503 T8349 T8340 prep with,generated
R5504 T8350 T8351 nummod 5.6,M
R5505 T8351 T8349 pobj M,with
R5506 T8352 T8350 cc and,5.6
R5507 T8353 T8350 conj 7,5.6
R5508 T8354 T8355 compound guanidine,hydrochloride
R5509 T8355 T8351 appos hydrochloride,M
R5510 T8356 T8355 cc and,hydrochloride
R5511 T8357 T8355 conj urea,hydrochloride
R5512 T8358 T8340 punct ", ",generated
R5513 T8359 T8340 advmod respectively,generated
R5514 T8360 T8340 punct .,generated
R5515 T8362 T8363 det The,conformers
R5516 T8363 T8365 nsubjpass conformers,diluted
R5517 T8364 T8363 compound Cox11,conformers
R5518 T8366 T8365 auxpass were,diluted
R5519 T8367 T8365 advmod then,diluted
R5520 T8368 T8369 punct ~,20-fold
R5521 T8369 T8365 advmod 20-fold,diluted
R5522 T8370 T8365 prep in,diluted
R5523 T8371 T8372 compound CHAPS,binding
R5524 T8372 T8374 compound binding,buffer
R5525 T8373 T8372 punct -,binding
R5526 T8374 T8370 pobj buffer,in
R5527 T8375 T8374 acl containing,buffer
R5528 T8376 T8377 compound GST,LD
R5529 T8377 T8375 dobj LD,containing
R5530 T8378 T8377 punct -,LD
R5531 T8379 T8365 punct .,diluted
R5532 T8381 T8382 compound Immunoprecipitation,assays
R5533 T8382 T8383 nsubjpass assays,performed
R5534 T8384 T8382 prep with,assays
R5535 T8385 T8386 nummod 5,μg
R5536 T8386 T8384 pobj μg,with
R5537 T8387 T8386 prep of,μg
R5538 T8388 T8387 pobj antibody,of
R5539 T8389 T8382 cc and,assays
R5540 T8390 T8391 compound Western,blots
R5541 T8391 T8382 conj blots,assays
R5542 T8392 T8393 punct (,ng
R5543 T8393 T8391 parataxis ng,blots
R5544 T8394 T8395 punct ~,400
R5545 T8395 T8393 nummod 400,ng
R5546 T8396 T8395 quantmod 200,400
R5547 T8397 T8395 punct –,400
R5548 T8398 T8399 punct /,ml
R5549 T8399 T8393 prep ml,ng
R5550 T8400 T8399 prep of,ml
R5551 T8401 T8400 pobj antibody,of
R5552 T8402 T8393 punct ),ng
R5553 T8403 T8383 auxpass were,performed
R5554 T8404 T8405 advmod exactly,described
R5555 T8405 T8383 advcl described,performed
R5556 T8406 T8405 mark as,described
R5557 T8407 T8405 advmod previously,described
R5558 T8408 T8409 punct [,13
R5559 T8409 T8405 parataxis 13,described
R5560 T8410 T8409 punct ],13
R5561 T8411 T8383 punct .,performed