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PMC:1626108 / 42588-42942 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T8119 0-4 NN denotes Site
T8121 4-5 HYPH denotes -
T8120 5-13 VBN denotes directed
T8123 14-17 CC denotes and
T8124 18-26 NN denotes deletion
T8122 27-38 NN denotes mutagenesis
T8125 39-42 CC denotes and
T8126 43-50 NN denotes plasmid
T8127 51-63 NN denotes construction
T8128 63-64 . denotes .
T8129 64-184 sentence denotes Deletion mutagenesis was carried out with pairs of primers against domains of interest described in the figure legends.
T8130 65-73 NN denotes Deletion
T8131 74-85 NN denotes mutagenesis
T8133 86-89 VBD denotes was
T8132 90-97 VBN denotes carried
T8134 98-101 RP denotes out
T8135 102-106 IN denotes with
T8136 107-112 NNS denotes pairs
T8137 113-115 IN denotes of
T8138 116-123 NNS denotes primers
T8139 124-131 IN denotes against
T8140 132-139 NNS denotes domains
T8141 140-142 IN denotes of
T8142 143-151 NN denotes interest
T8143 152-161 VBN denotes described
T8144 162-164 IN denotes in
T8145 165-168 DT denotes the
T8147 169-175 NN denotes figure
T8146 176-183 NNS denotes legends
T8148 183-184 . denotes .
T8149 184-294 sentence denotes PCR products were subcloned into pGEX-KG [64], HybriZAP pBD-GAL4 vector (Stratagene), and pBUTE [62] vectors.
T8150 185-188 NN denotes PCR
T8151 189-197 NNS denotes products
T8153 198-202 VBD denotes were
T8152 203-212 VBN denotes subcloned
T8154 213-217 IN denotes into
T8155 218-222 NN denotes pGEX
T8157 222-223 HYPH denotes -
T8156 223-225 NN denotes KG
T8158 226-227 -LRB- denotes [
T8159 227-229 CD denotes 64
T8160 229-230 -RRB- denotes ]
T8161 230-232 , denotes ,
T8162 232-240 NN denotes HybriZAP
T8164 241-244 NN denotes pBD
T8166 244-245 HYPH denotes -
T8165 245-249 NN denotes GAL4
T8163 250-256 NN denotes vector
T8167 257-258 -LRB- denotes (
T8168 258-268 NNP denotes Stratagene
T8169 268-269 -RRB- denotes )
T8170 269-271 , denotes ,
T8171 271-274 CC denotes and
T8172 275-280 NN denotes pBUTE
T8174 281-282 -LRB- denotes [
T8175 282-284 CD denotes 62
T8176 284-285 -RRB- denotes ]
T8173 286-293 NNS denotes vectors
T8177 293-294 . denotes .
T8178 294-354 sentence denotes GST-LDZIP alone comprised residues 447–483 of human RanBP2.
T8179 295-298 NN denotes GST
T8181 298-299 HYPH denotes -
T8180 299-304 NN denotes LDZIP
T8183 305-310 RB denotes alone
T8182 311-320 VBN denotes comprised
T8184 321-329 NNS denotes residues
T8185 330-333 CD denotes 447
T8186 333-334 SYM denotes –
T8187 334-337 CD denotes 483
T8188 338-340 IN denotes of
T8189 341-346 JJ denotes human
T8190 347-353 NN denotes RanBP2
T8191 353-354 . denotes .
R5354 T8119 T8120 npadvmod Site,directed
R5355 T8120 T8122 amod directed,mutagenesis
R5356 T8121 T8120 punct -,directed
R5357 T8123 T8120 cc and,directed
R5358 T8124 T8120 conj deletion,directed
R5359 T8125 T8122 cc and,mutagenesis
R5360 T8126 T8127 compound plasmid,construction
R5361 T8127 T8122 conj construction,mutagenesis
R5362 T8128 T8127 punct .,construction
R5363 T8130 T8131 compound Deletion,mutagenesis
R5364 T8131 T8132 nsubjpass mutagenesis,carried
R5365 T8133 T8132 auxpass was,carried
R5366 T8134 T8132 prt out,carried
R5367 T8135 T8132 prep with,carried
R5368 T8136 T8135 pobj pairs,with
R5369 T8137 T8136 prep of,pairs
R5370 T8138 T8137 pobj primers,of
R5371 T8139 T8138 prep against,primers
R5372 T8140 T8139 pobj domains,against
R5373 T8141 T8140 prep of,domains
R5374 T8142 T8141 pobj interest,of
R5375 T8143 T8140 acl described,domains
R5376 T8144 T8143 prep in,described
R5377 T8145 T8146 det the,legends
R5378 T8146 T8144 pobj legends,in
R5379 T8147 T8146 compound figure,legends
R5380 T8148 T8132 punct .,carried
R5381 T8150 T8151 compound PCR,products
R5382 T8151 T8152 nsubjpass products,subcloned
R5383 T8153 T8152 auxpass were,subcloned
R5384 T8154 T8152 prep into,subcloned
R5385 T8155 T8156 compound pGEX,KG
R5386 T8156 T8154 pobj KG,into
R5387 T8157 T8156 punct -,KG
R5388 T8158 T8159 punct [,64
R5389 T8159 T8156 parataxis 64,KG
R5390 T8160 T8159 punct ],64
R5391 T8161 T8156 punct ", ",KG
R5392 T8162 T8163 compound HybriZAP,vector
R5393 T8163 T8156 conj vector,KG
R5394 T8164 T8165 compound pBD,GAL4
R5395 T8165 T8163 compound GAL4,vector
R5396 T8166 T8165 punct -,GAL4
R5397 T8167 T8168 punct (,Stratagene
R5398 T8168 T8163 parataxis Stratagene,vector
R5399 T8169 T8168 punct ),Stratagene
R5400 T8170 T8163 punct ", ",vector
R5401 T8171 T8163 cc and,vector
R5402 T8172 T8173 nmod pBUTE,vectors
R5403 T8173 T8163 conj vectors,vector
R5404 T8174 T8175 punct [,62
R5405 T8175 T8172 parataxis 62,pBUTE
R5406 T8176 T8175 punct ],62
R5407 T8177 T8152 punct .,subcloned
R5408 T8179 T8180 compound GST,LDZIP
R5409 T8180 T8182 nsubj LDZIP,comprised
R5410 T8181 T8180 punct -,LDZIP
R5411 T8183 T8180 advmod alone,LDZIP
R5412 T8184 T8185 nmod residues,447
R5413 T8185 T8182 dobj 447,comprised
R5414 T8186 T8187 punct –,483
R5415 T8187 T8185 prep 483,447
R5416 T8188 T8185 prep of,447
R5417 T8189 T8190 amod human,RanBP2
R5418 T8190 T8188 pobj RanBP2,of
R5419 T8191 T8182 punct .,comprised