PMC:1626108 JSON TXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id	Subject	Object	Predicate	Lexical cue
T231	0-6	NN	denotes	RanBP2
T233	0-121	sentence	denotes	RanBP2 Modulates Cox11 and Hexokinase I Activities and Haploinsufficiency of RanBP2 Causes Deficits in Glucose Metabolism
T232	7-16	VBZ	denotes	Modulates
T234	17-22	NN	denotes	Cox11
T236	23-26	CC	denotes	and
T237	27-37	NN	denotes	Hexokinase
T238	38-39	CD	denotes	I
T235	40-50	NNS	denotes	Activities
T239	51-54	CC	denotes	and
T240	55-73	NN	denotes	Haploinsufficiency
T242	74-76	IN	denotes	of
T243	77-83	NN	denotes	RanBP2
T241	84-90	VBZ	denotes	Causes
T244	91-99	NNS	denotes	Deficits
T245	100-102	IN	denotes	in
T246	103-110	NN	denotes	Glucose
T247	111-121	NN	denotes	Metabolism
T248	121-255	sentence	denotes	RanBP2 Modulation of Glucose Catabolism Abstract The Ran-binding protein 2 (RanBP2) is a large multimodular and pleiotropic protein.
T249	172-175	DT	denotes	The
T251	176-179	NN	denotes	Ran
T253	179-180	HYPH	denotes	-
T252	180-187	VBG	denotes	binding
T250	188-195	NN	denotes	protein
T255	196-197	CD	denotes	2
T256	198-199	-LRB-	denotes	(
T257	199-205	NN	denotes	RanBP2
T258	205-206	-RRB-	denotes	)
T254	207-209	VBZ	denotes	is
T259	210-211	DT	denotes	a
T261	212-217	JJ	denotes	large
T262	218-230	JJ	denotes	multimodular
T263	231-234	CC	denotes	and
T264	235-246	JJ	denotes	pleiotropic
T260	247-254	NN	denotes	protein
T265	254-255	.	denotes	.
T266	255-386	sentence	denotes	Several molecular partners with distinct functions interacting specifically with selective modules of RanBP2 have been identified.
T267	256-263	JJ	denotes	Several
T269	264-273	JJ	denotes	molecular
T268	274-282	NNS	denotes	partners
T271	283-287	IN	denotes	with
T272	288-296	JJ	denotes	distinct
T273	297-306	NNS	denotes	functions
T274	307-318	VBG	denotes	interacting
T275	319-331	RB	denotes	specifically
T276	332-336	IN	denotes	with
T277	337-346	JJ	denotes	selective
T278	347-354	NNS	denotes	modules
T279	355-357	IN	denotes	of
T280	358-364	NN	denotes	RanBP2
T281	365-369	VBP	denotes	have
T282	370-374	VBN	denotes	been
T270	375-385	VBN	denotes	identified
T283	385-386	.	denotes	.
T284	386-534	sentence	denotes	Yet, the significance of these interactions with RanBP2 and the genetic and physiological role(s) of RanBP2 in a whole-animal model remain elusive.
T285	387-390	RB	denotes	Yet
T287	390-392	,	denotes	,
T288	392-395	DT	denotes	the
T289	396-408	NN	denotes	significance
T290	409-411	IN	denotes	of
T291	412-417	DT	denotes	these
T292	418-430	NNS	denotes	interactions
T293	431-435	IN	denotes	with
T294	436-442	NN	denotes	RanBP2
T295	443-446	CC	denotes	and
T296	447-450	DT	denotes	the
T298	451-458	JJ	denotes	genetic
T299	459-462	CC	denotes	and
T300	463-476	JJ	denotes	physiological
T297	477-481	NN	denotes	role
T301	481-482	-LRB-	denotes	(
T302	482-483	AFX	denotes	s
T303	483-484	-RRB-	denotes	)
T304	485-487	IN	denotes	of
T305	488-494	NN	denotes	RanBP2
T306	495-497	IN	denotes	in
T307	498-499	DT	denotes	a
T309	500-505	JJ	denotes	whole
T311	505-506	HYPH	denotes	-
T310	506-512	NN	denotes	animal
T308	513-518	NN	denotes	model
T286	519-525	VBP	denotes	remain
T312	526-533	JJ	denotes	elusive
T313	533-534	.	denotes	.
T314	534-660	sentence	denotes	Here, we report the identification of two novel partners of RanBP2 and a novel physiological role of RanBP2 in a mouse model.
T315	535-539	RB	denotes	Here
T317	539-541	,	denotes	,
T318	541-543	PRP	denotes	we
T316	544-550	VBP	denotes	report
T319	551-554	DT	denotes	the
T320	555-569	NN	denotes	identification
T321	570-572	IN	denotes	of
T322	573-576	CD	denotes	two
T324	577-582	JJ	denotes	novel
T323	583-591	NNS	denotes	partners
T325	592-594	IN	denotes	of
T326	595-601	NN	denotes	RanBP2
T327	602-605	CC	denotes	and
T328	606-607	DT	denotes	a
T330	608-613	JJ	denotes	novel
T331	614-627	JJ	denotes	physiological
T329	628-632	NN	denotes	role
T332	633-635	IN	denotes	of
T333	636-642	NN	denotes	RanBP2
T334	643-645	IN	denotes	in
T335	646-647	DT	denotes	a
T337	648-653	NN	denotes	mouse
T336	654-659	NN	denotes	model
T338	659-660	.	denotes	.
T339	660-849	sentence	denotes	RanBP2 associates in vitro and in vivo and colocalizes with the mitochondrial metallochaperone, Cox11, and the pacemaker of glycolysis, hexokinase type I (HKI) via its leucine-rich domain.
T340	661-667	NN	denotes	RanBP2
T341	668-678	VBZ	denotes	associates
T342	679-681	FW	denotes	in
T343	682-687	FW	denotes	vitro
T344	688-691	CC	denotes	and
T345	692-694	FW	denotes	in
T346	695-699	FW	denotes	vivo
T347	700-703	CC	denotes	and
T348	704-715	VBZ	denotes	colocalizes
T349	716-720	IN	denotes	with
T350	721-724	DT	denotes	the
T352	725-738	JJ	denotes	mitochondrial
T351	739-755	NN	denotes	metallochaperone
T353	755-757	,	denotes	,
T354	757-762	NN	denotes	Cox11
T355	762-764	,	denotes	,
T356	764-767	CC	denotes	and
T357	768-771	DT	denotes	the
T358	772-781	NN	denotes	pacemaker
T359	782-784	IN	denotes	of
T360	785-795	NN	denotes	glycolysis
T361	795-797	,	denotes	,
T362	797-807	NN	denotes	hexokinase
T363	808-812	NN	denotes	type
T364	813-814	CD	denotes	I
T365	815-816	-LRB-	denotes	(
T366	816-819	NN	denotes	HKI
T367	819-820	-RRB-	denotes	)
T368	821-824	IN	denotes	via
T369	825-828	PRP$	denotes	its
T371	829-836	NN	denotes	leucine
T373	836-837	HYPH	denotes	-
T372	837-841	JJ	denotes	rich
T370	842-848	NN	denotes	domain
T374	848-849	.	denotes	.
T375	849-1057	sentence	denotes	The leucine-rich domain of RanBP2 also exhibits strong chaperone activity toward intermediate and mature folding species of Cox11 supporting a chaperone role of RanBP2 in the cytosol during Cox11 biogenesis.
T376	850-853	DT	denotes	The
T378	854-861	NN	denotes	leucine
T380	861-862	HYPH	denotes	-
T379	862-866	JJ	denotes	rich
T377	867-873	NN	denotes	domain
T382	874-876	IN	denotes	of
T383	877-883	NN	denotes	RanBP2
T384	884-888	RB	denotes	also
T381	889-897	VBZ	denotes	exhibits
T385	898-904	JJ	denotes	strong
T387	905-914	NN	denotes	chaperone
T386	915-923	NN	denotes	activity
T388	924-930	IN	denotes	toward
T389	931-943	JJ	denotes	intermediate
T391	944-947	CC	denotes	and
T392	948-954	JJ	denotes	mature
T393	955-962	NN	denotes	folding
T390	963-970	NNS	denotes	species
T394	971-973	IN	denotes	of
T395	974-979	NN	denotes	Cox11
T396	980-990	VBG	denotes	supporting
T397	991-992	DT	denotes	a
T399	993-1002	NN	denotes	chaperone
T398	1003-1007	NN	denotes	role
T400	1008-1010	IN	denotes	of
T401	1011-1017	NN	denotes	RanBP2
T402	1018-1020	IN	denotes	in
T403	1021-1024	DT	denotes	the
T404	1025-1032	NN	denotes	cytosol
T405	1033-1039	IN	denotes	during
T406	1040-1045	NN	denotes	Cox11
T407	1046-1056	NN	denotes	biogenesis
T408	1056-1057	.	denotes	.
T409	1057-1159	sentence	denotes	Cox11 partially colocalizes with HKI, thus supporting additional and distinct roles in cell function.
T410	1058-1063	NN	denotes	Cox11
T412	1064-1073	RB	denotes	partially
T411	1074-1085	VBZ	denotes	colocalizes
T413	1086-1090	IN	denotes	with
T414	1091-1094	NN	denotes	HKI
T415	1094-1096	,	denotes	,
T416	1096-1100	RB	denotes	thus
T417	1101-1111	VBG	denotes	supporting
T418	1112-1122	JJ	denotes	additional
T420	1123-1126	CC	denotes	and
T421	1127-1135	JJ	denotes	distinct
T419	1136-1141	NNS	denotes	roles
T422	1142-1144	IN	denotes	in
T423	1145-1149	NN	denotes	cell
T424	1150-1158	NN	denotes	function
T425	1158-1159	.	denotes	.
T426	1159-1260	sentence	denotes	Cox11 is a strong inhibitor of HKI, and RanBP2 suppresses the inhibitory activity of Cox11 over HKI.
T427	1160-1165	NN	denotes	Cox11
T428	1166-1168	VBZ	denotes	is
T429	1169-1170	DT	denotes	a
T431	1171-1177	JJ	denotes	strong
T430	1178-1187	NN	denotes	inhibitor
T432	1188-1190	IN	denotes	of
T433	1191-1194	NN	denotes	HKI
T434	1194-1196	,	denotes	,
T435	1196-1199	CC	denotes	and
T436	1200-1206	NN	denotes	RanBP2
T437	1207-1217	VBZ	denotes	suppresses
T438	1218-1221	DT	denotes	the
T440	1222-1232	JJ	denotes	inhibitory
T439	1233-1241	NN	denotes	activity
T441	1242-1244	IN	denotes	of
T442	1245-1250	NN	denotes	Cox11
T443	1251-1255	IN	denotes	over
T444	1256-1259	NN	denotes	HKI
T445	1259-1260	.	denotes	.
T446	1260-1408	sentence	denotes	To probe the physiological role of RanBP2 and its role in HKI function, a mouse model harboring a genetically disrupted RanBP2 locus was generated.
T447	1261-1263	TO	denotes	To
T448	1264-1269	VB	denotes	probe
T450	1270-1273	DT	denotes	the
T452	1274-1287	JJ	denotes	physiological
T451	1288-1292	NN	denotes	role
T453	1293-1295	IN	denotes	of
T454	1296-1302	NN	denotes	RanBP2
T455	1303-1306	CC	denotes	and
T456	1307-1310	PRP$	denotes	its
T457	1311-1315	NN	denotes	role
T458	1316-1318	IN	denotes	in
T459	1319-1322	NN	denotes	HKI
T460	1323-1331	NN	denotes	function
T461	1331-1333	,	denotes	,
T462	1333-1334	DT	denotes	a
T464	1335-1340	NN	denotes	mouse
T463	1341-1346	NN	denotes	model
T465	1347-1356	VBG	denotes	harboring
T466	1357-1358	DT	denotes	a
T468	1359-1370	RB	denotes	genetically
T469	1371-1380	VBN	denotes	disrupted
T470	1381-1387	NN	denotes	RanBP2
T467	1388-1393	NN	denotes	locus
T471	1394-1397	VBD	denotes	was
T449	1398-1407	VBN	denotes	generated
T472	1407-1408	.	denotes	.
T473	1408-1591	sentence	denotes	RanBP2−/− are embryonically lethal, and haploinsufficiency of RanBP2 in an inbred strain causes a pronounced decrease of HKI and ATP levels selectively in the central nervous system.
T474	1409-1415	NN	denotes	RanBP2
T476	1415-1416	SYM	denotes	−
T477	1416-1417	HYPH	denotes	/
T478	1417-1418	SYM	denotes	−
T475	1419-1422	VBP	denotes	are
T479	1423-1436	RB	denotes	embryonically
T480	1437-1443	JJ	denotes	lethal
T481	1443-1445	,	denotes	,
T482	1445-1448	CC	denotes	and
T483	1449-1467	NN	denotes	haploinsufficiency
T485	1468-1470	IN	denotes	of
T486	1471-1477	NN	denotes	RanBP2
T487	1478-1480	IN	denotes	in
T488	1481-1483	DT	denotes	an
T490	1484-1490	JJ	denotes	inbred
T489	1491-1497	NN	denotes	strain
T484	1498-1504	VBZ	denotes	causes
T491	1505-1506	DT	denotes	a
T493	1507-1517	JJ	denotes	pronounced
T492	1518-1526	NN	denotes	decrease
T494	1527-1529	IN	denotes	of
T495	1530-1533	NN	denotes	HKI
T497	1534-1537	CC	denotes	and
T498	1538-1541	NN	denotes	ATP
T496	1542-1548	NNS	denotes	levels
T499	1549-1560	RB	denotes	selectively
T500	1561-1563	IN	denotes	in
T501	1564-1567	DT	denotes	the
T503	1568-1575	JJ	denotes	central
T504	1576-1583	JJ	denotes	nervous
T502	1584-1590	NN	denotes	system
T505	1590-1591	.	denotes	.
T506	1591-1732	sentence	denotes	Inbred RanBP2+/− mice also exhibit deficits in growth rates and glucose catabolism without impairment of glucose uptake and gluconeogenesis.
T507	1592-1598	JJ	denotes	Inbred
T509	1599-1605	NN	denotes	RanBP2
T510	1605-1606	SYM	denotes	+
T511	1606-1607	HYPH	denotes	/
T512	1607-1608	SYM	denotes	−
T508	1609-1613	NNS	denotes	mice
T514	1614-1618	RB	denotes	also
T513	1619-1626	VBP	denotes	exhibit
T515	1627-1635	NNS	denotes	deficits
T516	1636-1638	IN	denotes	in
T517	1639-1645	NN	denotes	growth
T518	1646-1651	NNS	denotes	rates
T519	1652-1655	CC	denotes	and
T520	1656-1663	NN	denotes	glucose
T521	1664-1674	NN	denotes	catabolism
T522	1675-1682	IN	denotes	without
T523	1683-1693	NN	denotes	impairment
T524	1694-1696	IN	denotes	of
T525	1697-1704	NN	denotes	glucose
T526	1705-1711	NN	denotes	uptake
T527	1712-1715	CC	denotes	and
T528	1716-1731	NN	denotes	gluconeogenesis
T529	1731-1732	.	denotes	.
T530	1732-1861	sentence	denotes	These phenotypes are accompanied by a decrease in the electrophysiological responses of photosensory and postreceptoral neurons.
T531	1733-1738	DT	denotes	These
T532	1739-1749	NNS	denotes	phenotypes
T534	1750-1753	VBP	denotes	are
T533	1754-1765	VBN	denotes	accompanied
T535	1766-1768	IN	denotes	by
T536	1769-1770	DT	denotes	a
T537	1771-1779	NN	denotes	decrease
T538	1780-1782	IN	denotes	in
T539	1783-1786	DT	denotes	the
T541	1787-1807	JJ	denotes	electrophysiological
T540	1808-1817	NNS	denotes	responses
T542	1818-1820	IN	denotes	of
T543	1821-1833	JJ	denotes	photosensory
T545	1834-1837	CC	denotes	and
T546	1838-1852	JJ	denotes	postreceptoral
T544	1853-1860	NNS	denotes	neurons
T547	1860-1861	.	denotes	.
T548	1861-2047	sentence	denotes	Hence, RanBP2 and its partners emerge as critical modulators of neuronal HKI, glucose catabolism, energy homeostasis, and targets for metabolic, aging disorders and allied neuropathies.
T549	1862-1867	RB	denotes	Hence
T551	1867-1869	,	denotes	,
T552	1869-1875	NN	denotes	RanBP2
T553	1876-1879	CC	denotes	and
T554	1880-1883	PRP$	denotes	its
T555	1884-1892	NNS	denotes	partners
T550	1893-1899	VBP	denotes	emerge
T556	1900-1902	IN	denotes	as
T557	1903-1911	JJ	denotes	critical
T558	1912-1922	NNS	denotes	modulators
T559	1923-1925	IN	denotes	of
T560	1926-1934	JJ	denotes	neuronal
T561	1935-1938	NN	denotes	HKI
T562	1938-1940	,	denotes	,
T563	1940-1947	NN	denotes	glucose
T564	1948-1958	NN	denotes	catabolism
T565	1958-1960	,	denotes	,
T566	1960-1966	NN	denotes	energy
T567	1967-1978	NN	denotes	homeostasis
T568	1978-1980	,	denotes	,
T569	1980-1983	CC	denotes	and
T570	1984-1991	NNS	denotes	targets
T571	1992-1995	IN	denotes	for
T572	1996-2005	JJ	denotes	metabolic
T574	2005-2007	,	denotes	,
T575	2007-2012	NN	denotes	aging
T573	2013-2022	NNS	denotes	disorders
T576	2023-2026	CC	denotes	and
T577	2027-2033	JJ	denotes	allied
T578	2034-2046	NNS	denotes	neuropathies
T579	2046-2047	.	denotes	.
T1039	3478-3481	DT	denotes	The
T1041	3482-3488	NN	denotes	RanBP2
T1042	3488-3489	HYPH	denotes	/
T1040	3489-3495	NN	denotes	Nup358
T1043	3496-3498	VBZ	denotes	is
T1044	3499-3500	DT	denotes	a
T1046	3501-3507	JJ	denotes	unique
T1047	3508-3518	NN	denotes	vertebrate
T1048	3519-3522	CC	denotes	and
T1049	3523-3528	JJ	denotes	large
T1050	3529-3537	NN	denotes	scaffold
T1045	3538-3545	NN	denotes	protein
T1051	3546-3555	VBN	denotes	comprised
T1052	3556-3558	IN	denotes	of
T1053	3559-3567	JJ	denotes	multiple
T1055	3568-3578	JJ	denotes	structural
T1056	3579-3582	CC	denotes	and
T1057	3583-3593	JJ	denotes	functional
T1054	3594-3601	NNS	denotes	domains
T1058	3602-3603	-LRB-	denotes	[
T1059	3603-3604	CD	denotes	1
T1060	3604-3605	SYM	denotes	–
T1061	3605-3606	CD	denotes	4
T1062	3606-3607	-RRB-	denotes	]
T1063	3607-3608	.	denotes	.
T1064	3608-3943	sentence	denotes	Several roles of RanBP2 have emerged that implicate RanBP2 in nucleocytoplasmic trafficking [3,5], protein biogenesis [6,7], the formation of the mitotic spindle, assembly of the nuclear envelope [8], and the integration of the nuclear envelope breakdown with kinetochore formation and maturation during early mitotic progression [9].
T1065	3609-3616	JJ	denotes	Several
T1066	3617-3622	NNS	denotes	roles
T1068	3623-3625	IN	denotes	of
T1069	3626-3632	NN	denotes	RanBP2
T1070	3633-3637	VBP	denotes	have
T1067	3638-3645	VBN	denotes	emerged
T1071	3646-3650	WDT	denotes	that
T1072	3651-3660	VBP	denotes	implicate
T1073	3661-3667	NN	denotes	RanBP2
T1074	3668-3670	IN	denotes	in
T1075	3671-3688	JJ	denotes	nucleocytoplasmic
T1076	3689-3700	NN	denotes	trafficking
T1077	3701-3702	-LRB-	denotes	[
T1079	3702-3703	CD	denotes	3
T1080	3703-3704	,	denotes	,
T1078	3704-3705	CD	denotes	5
T1081	3705-3706	-RRB-	denotes	]
T1082	3706-3708	,	denotes	,
T1083	3708-3715	NN	denotes	protein
T1084	3716-3726	NN	denotes	biogenesis
T1085	3727-3728	-LRB-	denotes	[
T1087	3728-3729	CD	denotes	6
T1088	3729-3730	,	denotes	,
T1086	3730-3731	CD	denotes	7
T1089	3731-3732	-RRB-	denotes	]
T1090	3732-3734	,	denotes	,
T1091	3734-3737	DT	denotes	the
T1092	3738-3747	NN	denotes	formation
T1093	3748-3750	IN	denotes	of
T1094	3751-3754	DT	denotes	the
T1096	3755-3762	JJ	denotes	mitotic
T1095	3763-3770	NN	denotes	spindle
T1097	3770-3772	,	denotes	,
T1098	3772-3780	NN	denotes	assembly
T1099	3781-3783	IN	denotes	of
T1100	3784-3787	DT	denotes	the
T1102	3788-3795	JJ	denotes	nuclear
T1101	3796-3804	NN	denotes	envelope
T1103	3805-3806	-LRB-	denotes	[
T1104	3806-3807	CD	denotes	8
T1105	3807-3808	-RRB-	denotes	]
T1106	3808-3810	,	denotes	,
T1107	3810-3813	CC	denotes	and
T1108	3814-3817	DT	denotes	the
T1109	3818-3829	NN	denotes	integration
T1110	3830-3832	IN	denotes	of
T1111	3833-3836	DT	denotes	the
T1113	3837-3844	JJ	denotes	nuclear
T1114	3845-3853	NN	denotes	envelope
T1112	3854-3863	NN	denotes	breakdown
T1115	3864-3868	IN	denotes	with
T1116	3869-3880	NN	denotes	kinetochore
T1117	3881-3890	NN	denotes	formation
T1118	3891-3894	CC	denotes	and
T1119	3895-3905	NN	denotes	maturation
T1120	3906-3912	IN	denotes	during
T1121	3913-3918	JJ	denotes	early
T1123	3919-3926	JJ	denotes	mitotic
T1122	3927-3938	NN	denotes	progression
T1124	3939-3940	-LRB-	denotes	[
T1125	3940-3941	CD	denotes	9
T1126	3941-3942	-RRB-	denotes	]
T1127	3942-3943	.	denotes	.
T1128	3943-4127	sentence	denotes	The specific interaction of RanBP2 with a diverse set of partners likely reflects a pleiotropic role of RanBP2 in cell function, possibly through the integration of multiple pathways.
T1129	3944-3947	DT	denotes	The
T1131	3948-3956	JJ	denotes	specific
T1130	3957-3968	NN	denotes	interaction
T1133	3969-3971	IN	denotes	of
T1134	3972-3978	NN	denotes	RanBP2
T1135	3979-3983	IN	denotes	with
T1136	3984-3985	DT	denotes	a
T1138	3986-3993	JJ	denotes	diverse
T1137	3994-3997	NN	denotes	set
T1139	3998-4000	IN	denotes	of
T1140	4001-4009	NNS	denotes	partners
T1141	4010-4016	RB	denotes	likely
T1132	4017-4025	VBZ	denotes	reflects
T1142	4026-4027	DT	denotes	a
T1144	4028-4039	JJ	denotes	pleiotropic
T1143	4040-4044	NN	denotes	role
T1145	4045-4047	IN	denotes	of
T1146	4048-4054	NN	denotes	RanBP2
T1147	4055-4057	IN	denotes	in
T1148	4058-4062	NN	denotes	cell
T1149	4063-4071	NN	denotes	function
T1150	4071-4073	,	denotes	,
T1151	4073-4081	RB	denotes	possibly
T1152	4082-4089	IN	denotes	through
T1153	4090-4093	DT	denotes	the
T1154	4094-4105	NN	denotes	integration
T1155	4106-4108	IN	denotes	of
T1156	4109-4117	JJ	denotes	multiple
T1157	4118-4126	NNS	denotes	pathways
T1158	4126-4127	.	denotes	.
T1159	4127-4271	sentence	denotes	On the other hand, the cell (tissue)-selective interaction of RanBP2 with some of its partners may also impart cell-restricted roles to RanBP2.
T1160	4128-4130	IN	denotes	On
T1162	4131-4134	DT	denotes	the
T1164	4135-4140	JJ	denotes	other
T1163	4141-4145	NN	denotes	hand
T1165	4145-4147	,	denotes	,
T1166	4147-4150	DT	denotes	the
T1168	4151-4155	NN	denotes	cell
T1170	4156-4157	-LRB-	denotes	(
T1171	4157-4163	NN	denotes	tissue
T1172	4163-4164	-RRB-	denotes	)
T1173	4164-4165	HYPH	denotes	-
T1169	4165-4174	JJ	denotes	selective
T1167	4175-4186	NN	denotes	interaction
T1174	4187-4189	IN	denotes	of
T1175	4190-4196	NN	denotes	RanBP2
T1176	4197-4201	IN	denotes	with
T1177	4202-4206	DT	denotes	some
T1178	4207-4209	IN	denotes	of
T1179	4210-4213	PRP$	denotes	its
T1180	4214-4222	NNS	denotes	partners
T1181	4223-4226	MD	denotes	may
T1182	4227-4231	RB	denotes	also
T1161	4232-4238	VB	denotes	impart
T1183	4239-4243	NN	denotes	cell
T1185	4243-4244	HYPH	denotes	-
T1184	4244-4254	JJ	denotes	restricted
T1186	4255-4260	NNS	denotes	roles
T1187	4261-4263	IN	denotes	to
T1188	4264-4270	NN	denotes	RanBP2
T1189	4270-4271	.	denotes	.
T1190	4271-4537	sentence	denotes	For example, the Ran-binding domains RBDn=1-4 of RanBP2 associate with the nuclear import co-receptor, importin-β [10,11], and antibodies against RanBP2 inhibit the nuclear import pathway in HeLa cells [3]; but such a role seems dispensable in Xenopus oocytes [12].
T1191	4272-4275	IN	denotes	For
T1193	4276-4283	NN	denotes	example
T1194	4283-4285	,	denotes	,
T1195	4285-4288	DT	denotes	the
T1197	4289-4292	NN	denotes	Ran
T1199	4292-4293	HYPH	denotes	-
T1198	4293-4300	VBG	denotes	binding
T1196	4301-4308	NNS	denotes	domains
T1200	4309-4313	NN	denotes	RBDn
T1202	4313-4314	SYM	denotes	=
T1201	4314-4315	CD	denotes	1
T1203	4315-4316	SYM	denotes	-
T1204	4316-4317	CD	denotes	4
T1205	4318-4320	IN	denotes	of
T1206	4321-4327	NN	denotes	RanBP2
T1192	4328-4337	VBP	denotes	associate
T1207	4338-4342	IN	denotes	with
T1208	4343-4346	DT	denotes	the
T1210	4347-4354	JJ	denotes	nuclear
T1211	4355-4361	NN	denotes	import
T1209	4362-4373	NN	denotes	co-receptor
T1212	4373-4375	,	denotes	,
T1213	4375-4383	NN	denotes	importin
T1215	4383-4384	HYPH	denotes	-
T1214	4384-4385	NN	denotes	β
T1216	4386-4387	-LRB-	denotes	[
T1218	4387-4389	CD	denotes	10
T1219	4389-4390	,	denotes	,
T1217	4390-4392	CD	denotes	11
T1220	4392-4393	-RRB-	denotes	]
T1221	4393-4395	,	denotes	,
T1222	4395-4398	CC	denotes	and
T1223	4399-4409	NNS	denotes	antibodies
T1225	4410-4417	IN	denotes	against
T1226	4418-4424	NN	denotes	RanBP2
T1224	4425-4432	VBP	denotes	inhibit
T1227	4433-4436	DT	denotes	the
T1229	4437-4444	JJ	denotes	nuclear
T1230	4445-4451	NN	denotes	import
T1228	4452-4459	NN	denotes	pathway
T1231	4460-4462	IN	denotes	in
T1232	4463-4467	NN	denotes	HeLa
T1233	4468-4473	NNS	denotes	cells
T1234	4474-4475	-LRB-	denotes	[
T1235	4475-4476	CD	denotes	3
T1236	4476-4477	-RRB-	denotes	]
T1237	4477-4478	:	denotes	;
T1238	4479-4482	CC	denotes	but
T1239	4483-4487	PDT	denotes	such
T1241	4488-4489	DT	denotes	a
T1240	4490-4494	NN	denotes	role
T1242	4495-4500	VBZ	denotes	seems
T1243	4501-4512	JJ	denotes	dispensable
T1244	4513-4515	IN	denotes	in
T1245	4516-4523	NN	denotes	Xenopus
T1246	4524-4531	NNS	denotes	oocytes
T1247	4532-4533	-LRB-	denotes	[
T1248	4533-4535	CD	denotes	12
T1249	4535-4536	-RRB-	denotes	]
T1250	4536-4537	.	denotes	.
T1251	4537-4981	sentence	denotes	In addition, the combination of the C-terminal domains, RBD4 and CY (of RanBP2), associates with a subset of G protein-coupled receptors, the red/green opsin, expressed in photosensory neurons and enhances opsin functional production [6,7], while the interaction of the KBD of RanBP2 with a subset of the conventional microtubule-based motor proteins, the kinesins, KIF5B and KIF5C, occurs selectively in the central nervous system (CNS) [13].
T1252	4538-4540	IN	denotes	In
T1254	4541-4549	NN	denotes	addition
T1255	4549-4551	,	denotes	,
T1256	4551-4554	DT	denotes	the
T1257	4555-4566	NN	denotes	combination
T1258	4567-4569	IN	denotes	of
T1259	4570-4573	DT	denotes	the
T1261	4574-4575	NN	denotes	C
T1263	4575-4576	HYPH	denotes	-
T1262	4576-4584	JJ	denotes	terminal
T1260	4585-4592	NNS	denotes	domains
T1264	4592-4594	,	denotes	,
T1265	4594-4598	NN	denotes	RBD4
T1266	4599-4602	CC	denotes	and
T1267	4603-4605	NN	denotes	CY
T1268	4606-4607	-LRB-	denotes	(
T1269	4607-4609	IN	denotes	of
T1270	4610-4616	NN	denotes	RanBP2
T1271	4616-4617	-RRB-	denotes	)
T1272	4617-4619	,	denotes	,
T1253	4619-4629	VBZ	denotes	associates
T1273	4630-4634	IN	denotes	with
T1274	4635-4636	DT	denotes	a
T1275	4637-4643	NN	denotes	subset
T1276	4644-4646	IN	denotes	of
T1277	4647-4648	NN	denotes	G
T1278	4649-4656	NN	denotes	protein
T1280	4656-4657	HYPH	denotes	-
T1279	4657-4664	VBN	denotes	coupled
T1281	4665-4674	NNS	denotes	receptors
T1282	4674-4676	,	denotes	,
T1283	4676-4679	DT	denotes	the
T1285	4680-4683	JJ	denotes	red
T1287	4683-4684	HYPH	denotes	/
T1286	4684-4689	JJ	denotes	green
T1284	4690-4695	NN	denotes	opsin
T1288	4695-4697	,	denotes	,
T1289	4697-4706	VBN	denotes	expressed
T1290	4707-4709	IN	denotes	in
T1291	4710-4722	JJ	denotes	photosensory
T1292	4723-4730	NNS	denotes	neurons
T1293	4731-4734	CC	denotes	and
T1294	4735-4743	VBZ	denotes	enhances
T1295	4744-4749	NN	denotes	opsin
T1297	4750-4760	JJ	denotes	functional
T1296	4761-4771	NN	denotes	production
T1298	4772-4773	-LRB-	denotes	[
T1300	4773-4774	CD	denotes	6
T1301	4774-4775	,	denotes	,
T1299	4775-4776	CD	denotes	7
T1302	4776-4777	-RRB-	denotes	]
T1303	4777-4779	,	denotes	,
T1304	4779-4784	IN	denotes	while
T1306	4785-4788	DT	denotes	the
T1307	4789-4800	NN	denotes	interaction
T1308	4801-4803	IN	denotes	of
T1309	4804-4807	DT	denotes	the
T1310	4808-4811	NN	denotes	KBD
T1311	4812-4814	IN	denotes	of
T1312	4815-4821	NN	denotes	RanBP2
T1313	4822-4826	IN	denotes	with
T1314	4827-4828	DT	denotes	a
T1315	4829-4835	NN	denotes	subset
T1316	4836-4838	IN	denotes	of
T1317	4839-4842	DT	denotes	the
T1319	4843-4855	JJ	denotes	conventional
T1320	4856-4867	NN	denotes	microtubule
T1322	4867-4868	HYPH	denotes	-
T1321	4868-4873	VBN	denotes	based
T1323	4874-4879	NN	denotes	motor
T1318	4880-4888	NN	denotes	proteins
T1324	4888-4890	,	denotes	,
T1325	4890-4893	DT	denotes	the
T1326	4894-4902	NNS	denotes	kinesins
T1327	4902-4904	,	denotes	,
T1328	4904-4909	NN	denotes	KIF5B
T1329	4910-4913	CC	denotes	and
T1330	4914-4919	NN	denotes	KIF5C
T1331	4919-4921	,	denotes	,
T1305	4921-4927	VBZ	denotes	occurs
T1332	4928-4939	RB	denotes	selectively
T1333	4940-4942	IN	denotes	in
T1334	4943-4946	DT	denotes	the
T1336	4947-4954	JJ	denotes	central
T1337	4955-4962	JJ	denotes	nervous
T1335	4963-4969	NN	denotes	system
T1338	4970-4971	-LRB-	denotes	(
T1339	4971-4974	NN	denotes	CNS
T1340	4974-4975	-RRB-	denotes	)
T1341	4976-4977	-LRB-	denotes	[
T1342	4977-4979	CD	denotes	13
T1343	4979-4980	-RRB-	denotes	]
T1344	4980-4981	.	denotes	.
T1345	4981-5157	sentence	denotes	A diverse set of additional molecular partners, each associating specifically with a selective domain of RanBP2, are likely to impart and integrate additional roles to RanBP2.
T1346	4982-4983	DT	denotes	A
T1348	4984-4991	JJ	denotes	diverse
T1347	4992-4995	NN	denotes	set
T1350	4996-4998	IN	denotes	of
T1351	4999-5009	JJ	denotes	additional
T1353	5010-5019	JJ	denotes	molecular
T1352	5020-5028	NNS	denotes	partners
T1354	5028-5030	,	denotes	,
T1355	5030-5034	RB	denotes	each
T1356	5035-5046	VBG	denotes	associating
T1357	5047-5059	RB	denotes	specifically
T1358	5060-5064	IN	denotes	with
T1359	5065-5066	DT	denotes	a
T1361	5067-5076	JJ	denotes	selective
T1360	5077-5083	NN	denotes	domain
T1362	5084-5086	IN	denotes	of
T1363	5087-5093	NN	denotes	RanBP2
T1364	5093-5095	,	denotes	,
T1349	5095-5098	VBP	denotes	are
T1365	5099-5105	JJ	denotes	likely
T1366	5106-5108	TO	denotes	to
T1367	5109-5115	VB	denotes	impart
T1368	5116-5119	CC	denotes	and
T1369	5120-5129	VB	denotes	integrate
T1370	5130-5140	JJ	denotes	additional
T1371	5141-5146	NNS	denotes	roles
T1372	5147-5149	IN	denotes	to
T1373	5150-5156	NN	denotes	RanBP2
T1374	5156-5157	.	denotes	.
T1375	5157-5474	sentence	denotes	For example, the cyclophilin-like domain (CLD), the internal repeat (W1W2/IR), and zinc-finger rich (ZnF) domains of RanBP2 associate specifically with components of the 19S cap of the proteasome [14], the E2 SUMO-1-conjugating enzyme (Ubc9) [15], and the nuclear export receptor, CRM1/exportin-1 [16], respectively.
T1376	5158-5161	IN	denotes	For
T1378	5162-5169	NN	denotes	example
T1379	5169-5171	,	denotes	,
T1380	5171-5174	DT	denotes	the
T1382	5175-5186	NN	denotes	cyclophilin
T1384	5186-5187	HYPH	denotes	-
T1383	5187-5191	JJ	denotes	like
T1381	5192-5198	NN	denotes	domain
T1385	5199-5200	-LRB-	denotes	(
T1386	5200-5203	NN	denotes	CLD
T1387	5203-5204	-RRB-	denotes	)
T1388	5204-5206	,	denotes	,
T1389	5206-5209	DT	denotes	the
T1391	5210-5218	JJ	denotes	internal
T1392	5219-5225	NN	denotes	repeat
T1393	5226-5227	-LRB-	denotes	(
T1395	5227-5231	NN	denotes	W1W2
T1396	5231-5232	HYPH	denotes	/
T1394	5232-5234	NN	denotes	IR
T1397	5234-5235	-RRB-	denotes	)
T1398	5235-5237	,	denotes	,
T1399	5237-5240	CC	denotes	and
T1400	5241-5245	NN	denotes	zinc
T1402	5245-5246	HYPH	denotes	-
T1401	5246-5252	NN	denotes	finger
T1403	5253-5257	JJ	denotes	rich
T1404	5258-5259	-LRB-	denotes	(
T1405	5259-5262	NN	denotes	ZnF
T1406	5262-5263	-RRB-	denotes	)
T1390	5264-5271	NNS	denotes	domains
T1407	5272-5274	IN	denotes	of
T1408	5275-5281	NN	denotes	RanBP2
T1377	5282-5291	VBP	denotes	associate
T1409	5292-5304	RB	denotes	specifically
T1410	5305-5309	IN	denotes	with
T1411	5310-5320	NNS	denotes	components
T1412	5321-5323	IN	denotes	of
T1413	5324-5327	DT	denotes	the
T1415	5328-5331	NN	denotes	19S
T1414	5332-5335	NN	denotes	cap
T1416	5336-5338	IN	denotes	of
T1417	5339-5342	DT	denotes	the
T1418	5343-5353	NN	denotes	proteasome
T1419	5354-5355	-LRB-	denotes	[
T1420	5355-5357	CD	denotes	14
T1421	5357-5358	-RRB-	denotes	]
T1422	5358-5360	,	denotes	,
T1423	5360-5363	DT	denotes	the
T1425	5364-5366	NN	denotes	E2
T1426	5367-5371	NN	denotes	SUMO
T1428	5371-5372	HYPH	denotes	-
T1429	5372-5373	CD	denotes	1
T1430	5373-5374	HYPH	denotes	-
T1427	5374-5385	VBG	denotes	conjugating
T1424	5386-5392	NN	denotes	enzyme
T1431	5393-5394	-LRB-	denotes	(
T1432	5394-5398	NN	denotes	Ubc9
T1433	5398-5399	-RRB-	denotes	)
T1434	5400-5401	-LRB-	denotes	[
T1435	5401-5403	CD	denotes	15
T1436	5403-5404	-RRB-	denotes	]
T1437	5404-5406	,	denotes	,
T1438	5406-5409	CC	denotes	and
T1439	5410-5413	DT	denotes	the
T1441	5414-5421	JJ	denotes	nuclear
T1442	5422-5428	NN	denotes	export
T1440	5429-5437	NN	denotes	receptor
T1443	5437-5439	,	denotes	,
T1444	5439-5443	NN	denotes	CRM1
T1446	5443-5444	HYPH	denotes	/
T1445	5444-5452	NN	denotes	exportin
T1447	5452-5453	HYPH	denotes	-
T1448	5453-5454	CD	denotes	1
T1449	5455-5456	-LRB-	denotes	[
T1450	5456-5458	CD	denotes	16
T1451	5458-5459	-RRB-	denotes	]
T1452	5459-5461	,	denotes	,
T1453	5461-5473	RB	denotes	respectively
T1454	5473-5474	.	denotes	.
T1455	5474-5756	sentence	denotes	RanBP2 itself was also found to exhibit SUMO1 E3 ligase activity [17], supporting a direct link between RanBP2-mediated SUMO-1 substrate modification and relocation of SUMO-1 modified cytosolic substrates (e.g., RanGAP) to the cytosolic face of the nuclear pore complex (NPC) [18].
T1456	5475-5481	NN	denotes	RanBP2
T1458	5482-5488	PRP	denotes	itself
T1459	5489-5492	VBD	denotes	was
T1460	5493-5497	RB	denotes	also
T1457	5498-5503	VBN	denotes	found
T1461	5504-5506	TO	denotes	to
T1462	5507-5514	VB	denotes	exhibit
T1463	5515-5520	NN	denotes	SUMO1
T1465	5521-5523	NN	denotes	E3
T1464	5524-5530	NN	denotes	ligase
T1466	5531-5539	NN	denotes	activity
T1467	5540-5541	-LRB-	denotes	[
T1468	5541-5543	CD	denotes	17
T1469	5543-5544	-RRB-	denotes	]
T1470	5544-5546	,	denotes	,
T1471	5546-5556	VBG	denotes	supporting
T1472	5557-5558	DT	denotes	a
T1474	5559-5565	JJ	denotes	direct
T1473	5566-5570	NN	denotes	link
T1475	5571-5578	IN	denotes	between
T1476	5579-5585	NN	denotes	RanBP2
T1478	5585-5586	HYPH	denotes	-
T1477	5586-5594	JJ	denotes	mediated
T1480	5595-5599	NN	denotes	SUMO
T1482	5599-5600	HYPH	denotes	-
T1483	5600-5601	CD	denotes	1
T1481	5602-5611	NN	denotes	substrate
T1479	5612-5624	NN	denotes	modification
T1484	5625-5628	CC	denotes	and
T1485	5629-5639	NN	denotes	relocation
T1486	5640-5642	IN	denotes	of
T1487	5643-5647	NN	denotes	SUMO
T1489	5647-5648	HYPH	denotes	-
T1490	5648-5649	CD	denotes	1
T1488	5650-5658	VBN	denotes	modified
T1492	5659-5668	JJ	denotes	cytosolic
T1491	5669-5679	NNS	denotes	substrates
T1493	5680-5681	-LRB-	denotes	(
T1494	5681-5685	FW	denotes	e.g.
T1496	5685-5687	,	denotes	,
T1495	5687-5693	NN	denotes	RanGAP
T1497	5693-5694	-RRB-	denotes	)
T1498	5695-5697	IN	denotes	to
T1499	5698-5701	DT	denotes	the
T1501	5702-5711	JJ	denotes	cytosolic
T1500	5712-5716	NN	denotes	face
T1502	5717-5719	IN	denotes	of
T1503	5720-5723	DT	denotes	the
T1505	5724-5731	JJ	denotes	nuclear
T1506	5732-5736	NN	denotes	pore
T1504	5737-5744	NN	denotes	complex
T1507	5745-5746	-LRB-	denotes	(
T1508	5746-5749	NN	denotes	NPC
T1509	5749-5750	-RRB-	denotes	)
T1510	5751-5752	-LRB-	denotes	[
T1511	5752-5754	CD	denotes	18
T1512	5754-5755	-RRB-	denotes	]
T1513	5755-5756	.	denotes	.
T1514	5756-6063	sentence	denotes	Finally, RanBP2 was also found to localize prominently to the mitochondria-rich ellipsoid subcellular compartment of photosensory neurons [19] and to RanGTPase-restricted foci along cytoplasmic tracks [19], in addition to its localization at cytoplasmic fibrils emanating from nuclear pores [2,3,10,12,19].
T1515	5757-5764	RB	denotes	Finally
T1517	5764-5766	,	denotes	,
T1518	5766-5772	NN	denotes	RanBP2
T1519	5773-5776	VBD	denotes	was
T1520	5777-5781	RB	denotes	also
T1516	5782-5787	VBN	denotes	found
T1521	5788-5790	TO	denotes	to
T1522	5791-5799	VB	denotes	localize
T1523	5800-5811	RB	denotes	prominently
T1524	5812-5814	IN	denotes	to
T1525	5815-5818	DT	denotes	the
T1527	5819-5831	NNS	denotes	mitochondria
T1529	5831-5832	HYPH	denotes	-
T1528	5832-5836	JJ	denotes	rich
T1530	5837-5846	JJ	denotes	ellipsoid
T1531	5847-5858	JJ	denotes	subcellular
T1526	5859-5870	NN	denotes	compartment
T1532	5871-5873	IN	denotes	of
T1533	5874-5886	JJ	denotes	photosensory
T1534	5887-5894	NNS	denotes	neurons
T1535	5895-5896	-LRB-	denotes	[
T1536	5896-5898	CD	denotes	19
T1537	5898-5899	-RRB-	denotes	]
T1538	5900-5903	CC	denotes	and
T1539	5904-5906	IN	denotes	to
T1540	5907-5916	NN	denotes	RanGTPase
T1542	5916-5917	HYPH	denotes	-
T1541	5917-5927	VBN	denotes	restricted
T1543	5928-5932	NNS	denotes	foci
T1544	5933-5938	IN	denotes	along
T1545	5939-5950	JJ	denotes	cytoplasmic
T1546	5951-5957	NNS	denotes	tracks
T1547	5958-5959	-LRB-	denotes	[
T1548	5959-5961	CD	denotes	19
T1549	5961-5962	-RRB-	denotes	]
T1550	5962-5964	,	denotes	,
T1551	5964-5966	IN	denotes	in
T1552	5967-5975	NN	denotes	addition
T1553	5976-5978	IN	denotes	to
T1554	5979-5982	PRP$	denotes	its
T1555	5983-5995	NN	denotes	localization
T1556	5996-5998	IN	denotes	at
T1557	5999-6010	JJ	denotes	cytoplasmic
T1558	6011-6018	NNS	denotes	fibrils
T1559	6019-6028	VBG	denotes	emanating
T1560	6029-6033	IN	denotes	from
T1561	6034-6041	JJ	denotes	nuclear
T1562	6042-6047	NNS	denotes	pores
T1563	6048-6049	-LRB-	denotes	[
T1565	6049-6050	CD	denotes	2
T1566	6050-6051	,	denotes	,
T1567	6051-6052	CD	denotes	3
T1568	6052-6053	,	denotes	,
T1569	6053-6055	CD	denotes	10
T1570	6055-6056	,	denotes	,
T1571	6056-6058	CD	denotes	12
T1572	6058-6059	,	denotes	,
T1564	6059-6061	CD	denotes	19
T1573	6061-6062	-RRB-	denotes	]
T1574	6062-6063	.	denotes	.
T1575	6063-6191	sentence	denotes	Emerging evidence supports that the CNS-selective effects of RanBP2 may also underlie the pathogenesis of certain neuropathies.
T1576	6064-6072	VBG	denotes	Emerging
T1577	6073-6081	NN	denotes	evidence
T1578	6082-6090	VBZ	denotes	supports
T1579	6091-6095	IN	denotes	that
T1581	6096-6099	DT	denotes	the
T1583	6100-6103	NN	denotes	CNS
T1585	6103-6104	HYPH	denotes	-
T1584	6104-6113	JJ	denotes	selective
T1582	6114-6121	NNS	denotes	effects
T1586	6122-6124	IN	denotes	of
T1587	6125-6131	NN	denotes	RanBP2
T1588	6132-6135	MD	denotes	may
T1589	6136-6140	RB	denotes	also
T1580	6141-6149	VB	denotes	underlie
T1590	6150-6153	DT	denotes	the
T1591	6154-6166	NN	denotes	pathogenesis
T1592	6167-6169	IN	denotes	of
T1593	6170-6177	JJ	denotes	certain
T1594	6178-6190	NNS	denotes	neuropathies
T1595	6190-6191	.	denotes	.
T1596	6191-6287	sentence	denotes	Parkin is ubiquitously expressed and interacts with the Ubc9-interacting domain of RanBP2 [20].
T1597	6192-6198	NN	denotes	Parkin
T1599	6199-6201	VBZ	denotes	is
T1600	6202-6214	RB	denotes	ubiquitously
T1598	6215-6224	VBN	denotes	expressed
T1601	6225-6228	CC	denotes	and
T1602	6229-6238	VBZ	denotes	interacts
T1603	6239-6243	IN	denotes	with
T1604	6244-6247	DT	denotes	the
T1606	6248-6252	NN	denotes	Ubc9
T1608	6252-6253	HYPH	denotes	-
T1607	6253-6264	VBG	denotes	interacting
T1605	6265-6271	NN	denotes	domain
T1609	6272-6274	IN	denotes	of
T1610	6275-6281	NN	denotes	RanBP2
T1611	6282-6283	-LRB-	denotes	[
T1612	6283-6285	CD	denotes	20
T1613	6285-6286	-RRB-	denotes	]
T1614	6286-6287	.	denotes	.
T1615	6287-6459	sentence	denotes	This promotes the ubiquitination and degradation of RanBP2 [20], possibly via the interaction of the CLD domain of RanBP2 with the 19S cap subunits of the proteasome [14].
T1616	6288-6292	DT	denotes	This
T1617	6293-6301	VBZ	denotes	promotes
T1618	6302-6305	DT	denotes	the
T1619	6306-6320	NN	denotes	ubiquitination
T1620	6321-6324	CC	denotes	and
T1621	6325-6336	NN	denotes	degradation
T1622	6337-6339	IN	denotes	of
T1623	6340-6346	NN	denotes	RanBP2
T1624	6347-6348	-LRB-	denotes	[
T1625	6348-6350	CD	denotes	20
T1626	6350-6351	-RRB-	denotes	]
T1627	6351-6353	,	denotes	,
T1628	6353-6361	RB	denotes	possibly
T1629	6362-6365	IN	denotes	via
T1630	6366-6369	DT	denotes	the
T1631	6370-6381	NN	denotes	interaction
T1632	6382-6384	IN	denotes	of
T1633	6385-6388	DT	denotes	the
T1635	6389-6392	NN	denotes	CLD
T1634	6393-6399	NN	denotes	domain
T1636	6400-6402	IN	denotes	of
T1637	6403-6409	NN	denotes	RanBP2
T1638	6410-6414	IN	denotes	with
T1639	6415-6418	DT	denotes	the
T1641	6419-6422	NN	denotes	19S
T1642	6423-6426	NN	denotes	cap
T1640	6427-6435	NNS	denotes	subunits
T1643	6436-6438	IN	denotes	of
T1644	6439-6442	DT	denotes	the
T1645	6443-6453	NN	denotes	proteasome
T1646	6454-6455	-LRB-	denotes	[
T1647	6455-6457	CD	denotes	14
T1648	6457-6458	-RRB-	denotes	]
T1649	6458-6459	.	denotes	.
T1650	6459-6656	sentence	denotes	Interestingly, the small yeast Ran-binding protein 1 (RanBP1), Yrb1p, with strong homology to the Ran-binding domains of RanBP2, is also required for cell-cycle regulated protein degradation [21].
T1651	6460-6473	RB	denotes	Interestingly
T1653	6473-6475	,	denotes	,
T1654	6475-6478	DT	denotes	the
T1656	6479-6484	JJ	denotes	small
T1657	6485-6490	NN	denotes	yeast
T1658	6491-6494	NN	denotes	Ran
T1660	6494-6495	HYPH	denotes	-
T1659	6495-6502	VBG	denotes	binding
T1655	6503-6510	NN	denotes	protein
T1661	6511-6512	CD	denotes	1
T1662	6513-6514	-LRB-	denotes	(
T1663	6514-6520	NN	denotes	RanBP1
T1664	6520-6521	-RRB-	denotes	)
T1665	6521-6523	,	denotes	,
T1666	6523-6528	NN	denotes	Yrb1p
T1667	6528-6530	,	denotes	,
T1668	6530-6534	IN	denotes	with
T1669	6535-6541	JJ	denotes	strong
T1670	6542-6550	NN	denotes	homology
T1671	6551-6553	IN	denotes	to
T1672	6554-6557	DT	denotes	the
T1674	6558-6561	NN	denotes	Ran
T1676	6561-6562	HYPH	denotes	-
T1675	6562-6569	VBG	denotes	binding
T1673	6570-6577	NNS	denotes	domains
T1677	6578-6580	IN	denotes	of
T1678	6581-6587	NN	denotes	RanBP2
T1679	6587-6589	,	denotes	,
T1680	6589-6591	VBZ	denotes	is
T1681	6592-6596	RB	denotes	also
T1652	6597-6605	VBN	denotes	required
T1682	6606-6609	IN	denotes	for
T1683	6610-6614	NN	denotes	cell
T1685	6614-6615	HYPH	denotes	-
T1684	6615-6620	NN	denotes	cycle
T1686	6621-6630	VBN	denotes	regulated
T1688	6631-6638	NN	denotes	protein
T1687	6639-6650	NN	denotes	degradation
T1689	6651-6652	-LRB-	denotes	[
T1690	6652-6654	CD	denotes	21
T1691	6654-6655	-RRB-	denotes	]
T1692	6655-6656	.	denotes	.
T1693	6656-6814	sentence	denotes	Parkin, like RanBP2 [17], has E3-ligase activity [22–24]; and loss-of-function of parkin leads to early onset autosomal recessive juvenile Parkinsonism [25].
T1694	6657-6663	NN	denotes	Parkin
T1696	6663-6665	,	denotes	,
T1697	6665-6669	IN	denotes	like
T1698	6670-6676	NN	denotes	RanBP2
T1699	6677-6678	-LRB-	denotes	[
T1700	6678-6680	CD	denotes	17
T1701	6680-6681	-RRB-	denotes	]
T1702	6681-6683	,	denotes	,
T1695	6683-6686	VBZ	denotes	has
T1703	6687-6689	NN	denotes	E3
T1705	6689-6690	HYPH	denotes	-
T1704	6690-6696	NN	denotes	ligase
T1706	6697-6705	NN	denotes	activity
T1707	6706-6707	-LRB-	denotes	[
T1708	6707-6709	CD	denotes	22
T1709	6709-6710	SYM	denotes	–
T1710	6710-6712	CD	denotes	24
T1711	6712-6713	-RRB-	denotes	]
T1712	6713-6714	:	denotes	;
T1713	6715-6718	CC	denotes	and
T1714	6719-6723	NN	denotes	loss
T1716	6723-6724	HYPH	denotes	-
T1717	6724-6726	IN	denotes	of
T1718	6726-6727	HYPH	denotes	-
T1719	6727-6735	NN	denotes	function
T1720	6736-6738	IN	denotes	of
T1721	6739-6745	NN	denotes	parkin
T1715	6746-6751	VBZ	denotes	leads
T1722	6752-6754	IN	denotes	to
T1723	6755-6760	JJ	denotes	early
T1724	6761-6766	NN	denotes	onset
T1726	6767-6776	JJ	denotes	autosomal
T1727	6777-6786	JJ	denotes	recessive
T1728	6787-6795	JJ	denotes	juvenile
T1725	6796-6808	NN	denotes	Parkinsonism
T1729	6809-6810	-LRB-	denotes	[
T1730	6810-6812	CD	denotes	25
T1731	6812-6813	-RRB-	denotes	]
T1732	6813-6814	.	denotes	.
T1733	6814-7234	sentence	denotes	While the dopaminergic neuronal-restricted effects of mutations in parkin are not understood [25], parkin−/− mice exhibit mitochondrial dysfunction and energy and growth deficits, and a fraction of parkin localizes to the cytoplasmic face of the mitochondria, where together with other partners is thought to promote the degradation of selective mitochondrial substrates and to exert a neuroprotective function [26–29].
T1734	6815-6820	IN	denotes	While
T1736	6821-6824	DT	denotes	the
T1738	6825-6837	JJ	denotes	dopaminergic
T1739	6838-6846	JJ	denotes	neuronal
T1741	6846-6847	HYPH	denotes	-
T1740	6847-6857	VBN	denotes	restricted
T1737	6858-6865	NNS	denotes	effects
T1742	6866-6868	IN	denotes	of
T1743	6869-6878	NNS	denotes	mutations
T1744	6879-6881	IN	denotes	in
T1745	6882-6888	NN	denotes	parkin
T1735	6889-6892	VBP	denotes	are
T1747	6893-6896	RB	denotes	not
T1748	6897-6907	JJ	denotes	understood
T1749	6908-6909	-LRB-	denotes	[
T1750	6909-6911	CD	denotes	25
T1751	6911-6912	-RRB-	denotes	]
T1752	6912-6914	,	denotes	,
T1753	6914-6920	NN	denotes	parkin
T1755	6920-6921	SYM	denotes	−
T1756	6921-6922	HYPH	denotes	/
T1757	6922-6923	SYM	denotes	−
T1754	6924-6928	NNS	denotes	mice
T1746	6929-6936	VBP	denotes	exhibit
T1758	6937-6950	JJ	denotes	mitochondrial
T1759	6951-6962	NN	denotes	dysfunction
T1760	6963-6966	CC	denotes	and
T1761	6967-6973	NN	denotes	energy
T1763	6974-6977	CC	denotes	and
T1764	6978-6984	NN	denotes	growth
T1762	6985-6993	NNS	denotes	deficits
T1765	6993-6995	,	denotes	,
T1766	6995-6998	CC	denotes	and
T1767	6999-7000	DT	denotes	a
T1768	7001-7009	NN	denotes	fraction
T1770	7010-7012	IN	denotes	of
T1771	7013-7019	NN	denotes	parkin
T1769	7020-7029	VBZ	denotes	localizes
T1772	7030-7032	IN	denotes	to
T1773	7033-7036	DT	denotes	the
T1775	7037-7048	JJ	denotes	cytoplasmic
T1774	7049-7053	NN	denotes	face
T1776	7054-7056	IN	denotes	of
T1777	7057-7060	DT	denotes	the
T1778	7061-7073	NNS	denotes	mitochondria
T1779	7073-7075	,	denotes	,
T1780	7075-7080	WRB	denotes	where
T1782	7081-7089	RB	denotes	together
T1783	7090-7094	IN	denotes	with
T1784	7095-7100	JJ	denotes	other
T1785	7101-7109	NNS	denotes	partners
T1786	7110-7112	VBZ	denotes	is
T1781	7113-7120	VBN	denotes	thought
T1787	7121-7123	TO	denotes	to
T1788	7124-7131	VB	denotes	promote
T1789	7132-7135	DT	denotes	the
T1790	7136-7147	NN	denotes	degradation
T1791	7148-7150	IN	denotes	of
T1792	7151-7160	JJ	denotes	selective
T1794	7161-7174	JJ	denotes	mitochondrial
T1793	7175-7185	NNS	denotes	substrates
T1795	7186-7189	CC	denotes	and
T1796	7190-7192	TO	denotes	to
T1797	7193-7198	VB	denotes	exert
T1798	7199-7200	DT	denotes	a
T1800	7201-7216	JJ	denotes	neuroprotective
T1799	7217-7225	NN	denotes	function
T1801	7226-7227	-LRB-	denotes	[
T1802	7227-7229	CD	denotes	26
T1803	7229-7230	SYM	denotes	–
T1804	7230-7232	CD	denotes	29
T1805	7232-7233	-RRB-	denotes	]
T1806	7233-7234	.	denotes	.
T1807	7234-7372	sentence	denotes	These data suggest that deficits in RanBP2 and parkin may share (patho) physiological pathways and support a multifaceted role of RanBP2.
T1808	7235-7240	DT	denotes	These
T1809	7241-7245	NNS	denotes	data
T1810	7246-7253	VBP	denotes	suggest
T1811	7254-7258	IN	denotes	that
T1813	7259-7267	NNS	denotes	deficits
T1814	7268-7270	IN	denotes	in
T1815	7271-7277	NN	denotes	RanBP2
T1816	7278-7281	CC	denotes	and
T1817	7282-7288	NN	denotes	parkin
T1818	7289-7292	MD	denotes	may
T1812	7293-7298	VB	denotes	share
T1819	7299-7300	-LRB-	denotes	(
T1821	7300-7305	AFX	denotes	patho
T1822	7305-7306	-RRB-	denotes	)
T1820	7307-7320	JJ	denotes	physiological
T1823	7321-7329	NNS	denotes	pathways
T1824	7330-7333	CC	denotes	and
T1825	7334-7341	VB	denotes	support
T1826	7342-7343	DT	denotes	a
T1828	7344-7356	JJ	denotes	multifaceted
T1827	7357-7361	NN	denotes	role
T1829	7362-7364	IN	denotes	of
T1830	7365-7371	NN	denotes	RanBP2
T1831	7371-7372	.	denotes	.
T1832	7372-7448	sentence	denotes	Yet clear functions of RanBP2 in animal and cell physiology remain elusive.
T1833	7373-7376	RB	denotes	Yet
T1835	7377-7382	JJ	denotes	clear
T1836	7383-7392	NNS	denotes	functions
T1837	7393-7395	IN	denotes	of
T1838	7396-7402	NN	denotes	RanBP2
T1839	7403-7405	IN	denotes	in
T1840	7406-7412	NN	denotes	animal
T1842	7413-7416	CC	denotes	and
T1843	7417-7421	NN	denotes	cell
T1841	7422-7432	NN	denotes	physiology
T1834	7433-7439	VBP	denotes	remain
T1844	7440-7447	JJ	denotes	elusive
T1845	7447-7448	.	denotes	.
T1846	7448-7769	sentence	denotes	Here, we report on the identification and function of two novel partners of RanBP2, Cox11 and hexokinase type I (HKI), which interact with a large and orphan domain of RanBP2, the leucine-rich domain (LD); and on the outcome of partial loss-of-function of RanBP2 on HKI, animal physiology, and glucose/energy metabolism.
T1847	7449-7453	RB	denotes	Here
T1849	7453-7455	,	denotes	,
T1850	7455-7457	PRP	denotes	we
T1848	7458-7464	VBP	denotes	report
T1851	7465-7467	IN	denotes	on
T1852	7468-7471	DT	denotes	the
T1853	7472-7486	NN	denotes	identification
T1854	7487-7490	CC	denotes	and
T1855	7491-7499	NN	denotes	function
T1856	7500-7502	IN	denotes	of
T1857	7503-7506	CD	denotes	two
T1859	7507-7512	JJ	denotes	novel
T1858	7513-7521	NNS	denotes	partners
T1860	7522-7524	IN	denotes	of
T1861	7525-7531	NN	denotes	RanBP2
T1862	7531-7533	,	denotes	,
T1863	7533-7538	NN	denotes	Cox11
T1864	7539-7542	CC	denotes	and
T1865	7543-7553	NN	denotes	hexokinase
T1866	7554-7558	NN	denotes	type
T1867	7559-7560	CD	denotes	I
T1868	7561-7562	-LRB-	denotes	(
T1869	7562-7565	NN	denotes	HKI
T1870	7565-7566	-RRB-	denotes	)
T1871	7566-7568	,	denotes	,
T1872	7568-7573	WDT	denotes	which
T1873	7574-7582	VBP	denotes	interact
T1874	7583-7587	IN	denotes	with
T1875	7588-7589	DT	denotes	a
T1877	7590-7595	JJ	denotes	large
T1878	7596-7599	CC	denotes	and
T1879	7600-7606	NN	denotes	orphan
T1876	7607-7613	NN	denotes	domain
T1880	7614-7616	IN	denotes	of
T1881	7617-7623	NN	denotes	RanBP2
T1882	7623-7625	,	denotes	,
T1883	7625-7628	DT	denotes	the
T1885	7629-7636	NN	denotes	leucine
T1887	7636-7637	HYPH	denotes	-
T1886	7637-7641	JJ	denotes	rich
T1884	7642-7648	NN	denotes	domain
T1888	7649-7650	-LRB-	denotes	(
T1889	7650-7652	NN	denotes	LD
T1890	7652-7653	-RRB-	denotes	)
T1891	7653-7654	:	denotes	;
T1892	7655-7658	CC	denotes	and
T1893	7659-7661	IN	denotes	on
T1894	7662-7665	DT	denotes	the
T1895	7666-7673	NN	denotes	outcome
T1896	7674-7676	IN	denotes	of
T1897	7677-7684	JJ	denotes	partial
T1898	7685-7689	NN	denotes	loss
T1899	7689-7690	HYPH	denotes	-
T1900	7690-7692	IN	denotes	of
T1901	7692-7693	HYPH	denotes	-
T1902	7693-7701	NN	denotes	function
T1903	7702-7704	IN	denotes	of
T1904	7705-7711	NN	denotes	RanBP2
T1905	7712-7714	IN	denotes	on
T1906	7715-7718	NN	denotes	HKI
T1907	7718-7720	,	denotes	,
T1908	7720-7726	NN	denotes	animal
T1909	7727-7737	NN	denotes	physiology
T1910	7737-7739	,	denotes	,
T1911	7739-7742	CC	denotes	and
T1912	7743-7750	NN	denotes	glucose
T1914	7750-7751	HYPH	denotes	/
T1913	7751-7757	NN	denotes	energy
T1915	7758-7768	NN	denotes	metabolism
T1916	7768-7769	.	denotes	.
T2102	7780-7783	DT	denotes	The
T2103	7784-7786	NN	denotes	LD
T2105	7787-7789	IN	denotes	of
T2106	7790-7796	NN	denotes	RanBP2
T2104	7797-7806	VBZ	denotes	Interacts
T2107	7807-7811	IN	denotes	with
T2108	7812-7817	NN	denotes	Cox11
T2109	7818-7821	CC	denotes	and
T2110	7822-7825	NN	denotes	HKI
T2111	7825-7981	sentence	denotes	The LD of RanBP2 (Figure 1A) is a large and orphan domain of ~700 residues (~80 kDa), for which no molecular partners have been identified until this date.
T2112	7826-7829	DT	denotes	The
T2113	7830-7832	NN	denotes	LD
T2115	7833-7835	IN	denotes	of
T2116	7836-7842	NN	denotes	RanBP2
T2117	7843-7844	-LRB-	denotes	(
T2119	7844-7850	NN	denotes	Figure
T2118	7851-7853	NN	denotes	1A
T2120	7853-7854	-RRB-	denotes	)
T2114	7855-7857	VBZ	denotes	is
T2121	7858-7859	DT	denotes	a
T2123	7860-7865	JJ	denotes	large
T2124	7866-7869	CC	denotes	and
T2125	7870-7876	NN	denotes	orphan
T2122	7877-7883	NN	denotes	domain
T2126	7884-7886	IN	denotes	of
T2127	7887-7888	SYM	denotes	~
T2128	7888-7891	CD	denotes	700
T2129	7892-7900	NNS	denotes	residues
T2130	7901-7902	-LRB-	denotes	(
T2131	7902-7903	SYM	denotes	~
T2132	7903-7905	CD	denotes	80
T2133	7906-7909	NN	denotes	kDa
T2134	7909-7910	-RRB-	denotes	)
T2135	7910-7912	,	denotes	,
T2136	7912-7915	IN	denotes	for
T2138	7916-7921	WDT	denotes	which
T2139	7922-7924	DT	denotes	no
T2141	7925-7934	JJ	denotes	molecular
T2140	7935-7943	NNS	denotes	partners
T2142	7944-7948	VBP	denotes	have
T2143	7949-7953	VBN	denotes	been
T2137	7954-7964	VBN	denotes	identified
T2144	7965-7970	IN	denotes	until
T2145	7971-7975	DT	denotes	this
T2146	7976-7980	NN	denotes	date
T2147	7980-7981	.	denotes	.
T2148	7981-8048	sentence	denotes	Brain and retina yeast two-hybrid libraries were screened with LD.
T2149	7982-7987	NN	denotes	Brain
T2151	7988-7991	CC	denotes	and
T2152	7992-7998	NN	denotes	retina
T2153	7999-8004	NN	denotes	yeast
T2154	8005-8008	CD	denotes	two
T2156	8008-8009	HYPH	denotes	-
T2155	8009-8015	NN	denotes	hybrid
T2150	8016-8025	NNS	denotes	libraries
T2158	8026-8030	VBD	denotes	were
T2157	8031-8039	VBN	denotes	screened
T2159	8040-8044	IN	denotes	with
T2160	8045-8047	NN	denotes	LD
T2161	8047-8048	.	denotes	.
T2162	8048-8117	sentence	denotes	Cox11 was identified as a partner to this domain (Figure 1B and 1C).
T2163	8049-8054	NN	denotes	Cox11
T2165	8055-8058	VBD	denotes	was
T2164	8059-8069	VBN	denotes	identified
T2166	8070-8072	IN	denotes	as
T2167	8073-8074	DT	denotes	a
T2168	8075-8082	NN	denotes	partner
T2169	8083-8085	IN	denotes	to
T2170	8086-8090	DT	denotes	this
T2171	8091-8097	NN	denotes	domain
T2172	8098-8099	-LRB-	denotes	(
T2174	8099-8105	NN	denotes	Figure
T2173	8106-8108	NN	denotes	1B
T2175	8109-8112	CC	denotes	and
T2176	8113-8115	NN	denotes	1C
T2177	8115-8116	-RRB-	denotes	)
T2178	8116-8117	.	denotes	.
T2179	8117-8198	sentence	denotes	Cox11 is a metallochaperone implicated in cytochrome c oxidase assembly [30,31].
T2180	8118-8123	NN	denotes	Cox11
T2181	8124-8126	VBZ	denotes	is
T2182	8127-8128	DT	denotes	a
T2183	8129-8145	NN	denotes	metallochaperone
T2184	8146-8156	VBN	denotes	implicated
T2185	8157-8159	IN	denotes	in
T2186	8160-8170	NN	denotes	cytochrome
T2188	8171-8172	NN	denotes	c
T2187	8173-8180	NN	denotes	oxidase
T2189	8181-8189	NN	denotes	assembly
T2190	8190-8191	-LRB-	denotes	[
T2192	8191-8193	CD	denotes	30
T2193	8193-8194	,	denotes	,
T2191	8194-8196	CD	denotes	31
T2194	8196-8197	-RRB-	denotes	]
T2195	8197-8198	.	denotes	.
T2196	8198-8428	sentence	denotes	Structure-function analysis of the interaction between mCox11, LD of RanBP2, and subdomains thereof, with quantitative yeast two-hybrid assays [32], showed optimal interaction between the intact LD and Cox11 proteins (Figure 1C).
T2197	8199-8208	NN	denotes	Structure
T2199	8208-8209	HYPH	denotes	-
T2198	8209-8217	NN	denotes	function
T2200	8218-8226	NN	denotes	analysis
T2202	8227-8229	IN	denotes	of
T2203	8230-8233	DT	denotes	the
T2204	8234-8245	NN	denotes	interaction
T2205	8246-8253	IN	denotes	between
T2206	8254-8260	NN	denotes	mCox11
T2207	8260-8262	,	denotes	,
T2208	8262-8264	NN	denotes	LD
T2209	8265-8267	IN	denotes	of
T2210	8268-8274	NN	denotes	RanBP2
T2211	8274-8276	,	denotes	,
T2212	8276-8279	CC	denotes	and
T2213	8280-8290	NNS	denotes	subdomains
T2214	8291-8298	RB	denotes	thereof
T2215	8298-8300	,	denotes	,
T2216	8300-8304	IN	denotes	with
T2217	8305-8317	JJ	denotes	quantitative
T2219	8318-8323	NN	denotes	yeast
T2220	8324-8327	CD	denotes	two
T2222	8327-8328	HYPH	denotes	-
T2221	8328-8334	NN	denotes	hybrid
T2218	8335-8341	NNS	denotes	assays
T2223	8342-8343	-LRB-	denotes	[
T2224	8343-8345	CD	denotes	32
T2225	8345-8346	-RRB-	denotes	]
T2226	8346-8348	,	denotes	,
T2201	8348-8354	VBD	denotes	showed
T2227	8355-8362	JJ	denotes	optimal
T2228	8363-8374	NN	denotes	interaction
T2229	8375-8382	IN	denotes	between
T2230	8383-8386	DT	denotes	the
T2232	8387-8393	JJ	denotes	intact
T2233	8394-8396	NN	denotes	LD
T2234	8397-8400	CC	denotes	and
T2235	8401-8406	NN	denotes	Cox11
T2231	8407-8415	NN	denotes	proteins
T2236	8416-8417	-LRB-	denotes	(
T2238	8417-8423	NN	denotes	Figure
T2237	8424-8426	NN	denotes	1C
T2239	8426-8427	-RRB-	denotes	)
T2240	8427-8428	.	denotes	.
T2241	8428-8640	sentence	denotes	Pull-down assays of retinal extracts with glutathione S-transferase (GST)-LD precipitates a sodium dodecyl sulfate-resistant dimer isoform of Cox11 (Figure 1D, top panel), which does not bind to GST-LDZIP alone.
T2242	8429-8433	VB	denotes	Pull
T2244	8433-8434	HYPH	denotes	-
T2245	8434-8438	RP	denotes	down
T2243	8439-8445	NNS	denotes	assays
T2247	8446-8448	IN	denotes	of
T2248	8449-8456	JJ	denotes	retinal
T2249	8457-8465	NNS	denotes	extracts
T2250	8466-8470	IN	denotes	with
T2251	8471-8482	NN	denotes	glutathione
T2253	8483-8484	NN	denotes	S
T2254	8484-8485	HYPH	denotes	-
T2252	8485-8496	NN	denotes	transferase
T2256	8497-8498	-LRB-	denotes	(
T2257	8498-8501	NN	denotes	GST
T2258	8501-8502	-RRB-	denotes	)
T2259	8502-8503	HYPH	denotes	-
T2255	8503-8505	NN	denotes	LD
T2246	8506-8518	VBZ	denotes	precipitates
T2260	8519-8520	DT	denotes	a
T2262	8521-8527	NN	denotes	sodium
T2264	8528-8535	NN	denotes	dodecyl
T2263	8536-8543	NN	denotes	sulfate
T2266	8543-8544	HYPH	denotes	-
T2265	8544-8553	JJ	denotes	resistant
T2267	8554-8559	NN	denotes	dimer
T2261	8560-8567	NN	denotes	isoform
T2268	8568-8570	IN	denotes	of
T2269	8571-8576	NN	denotes	Cox11
T2270	8577-8578	-LRB-	denotes	(
T2272	8578-8584	NN	denotes	Figure
T2273	8585-8587	NN	denotes	1D
T2274	8587-8589	,	denotes	,
T2275	8589-8592	JJ	denotes	top
T2271	8593-8598	NN	denotes	panel
T2276	8598-8599	-RRB-	denotes	)
T2277	8599-8601	,	denotes	,
T2278	8601-8606	WDT	denotes	which
T2280	8607-8611	VBZ	denotes	does
T2281	8612-8615	RB	denotes	not
T2279	8616-8620	VB	denotes	bind
T2282	8621-8623	IN	denotes	to
T2283	8624-8627	NN	denotes	GST
T2285	8627-8628	HYPH	denotes	-
T2284	8628-8633	NN	denotes	LDZIP
T2286	8634-8639	RB	denotes	alone
T2287	8639-8640	.	denotes	.
T2288	8640-8855	sentence	denotes	In addition to Cox11, we also found that other mitochondrial components such as the outer membrane-associated protein, HKI [33] (Figure 1D, bottom panel) and mHsp70 (unpublished data), associated with LD of RanBP2.
T2289	8641-8643	IN	denotes	In
T2291	8644-8652	NN	denotes	addition
T2292	8653-8655	IN	denotes	to
T2293	8656-8661	NN	denotes	Cox11
T2294	8661-8663	,	denotes	,
T2295	8663-8665	PRP	denotes	we
T2296	8666-8670	RB	denotes	also
T2290	8671-8676	VBD	denotes	found
T2297	8677-8681	IN	denotes	that
T2299	8682-8687	JJ	denotes	other
T2301	8688-8701	JJ	denotes	mitochondrial
T2300	8702-8712	NNS	denotes	components
T2302	8713-8717	JJ	denotes	such
T2303	8718-8720	IN	denotes	as
T2304	8721-8724	DT	denotes	the
T2306	8725-8730	JJ	denotes	outer
T2307	8731-8739	NN	denotes	membrane
T2309	8739-8740	HYPH	denotes	-
T2308	8740-8750	VBN	denotes	associated
T2305	8751-8758	NN	denotes	protein
T2310	8758-8760	,	denotes	,
T2311	8760-8763	NN	denotes	HKI
T2312	8764-8765	-LRB-	denotes	[
T2313	8765-8767	CD	denotes	33
T2314	8767-8768	-RRB-	denotes	]
T2315	8769-8770	-LRB-	denotes	(
T2317	8770-8776	NN	denotes	Figure
T2318	8777-8779	NN	denotes	1D
T2319	8779-8781	,	denotes	,
T2320	8781-8787	JJ	denotes	bottom
T2316	8788-8793	NN	denotes	panel
T2321	8793-8794	-RRB-	denotes	)
T2322	8795-8798	CC	denotes	and
T2323	8799-8805	NN	denotes	mHsp70
T2324	8806-8807	-LRB-	denotes	(
T2326	8807-8818	JJ	denotes	unpublished
T2325	8819-8823	NNS	denotes	data
T2327	8823-8824	-RRB-	denotes	)
T2328	8824-8826	,	denotes	,
T2298	8826-8836	VBD	denotes	associated
T2329	8837-8841	IN	denotes	with
T2330	8842-8844	NN	denotes	LD
T2331	8845-8847	IN	denotes	of
T2332	8848-8854	NN	denotes	RanBP2
T2333	8854-8855	.	denotes	.
T2334	8855-9012	sentence	denotes	This association was highly specific toward the HKI isoform, because HKII, HKIII, and glucokinase did not interact with the LD of RanBP2 (unpublished data).
T2335	8856-8860	DT	denotes	This
T2336	8861-8872	NN	denotes	association
T2337	8873-8876	VBD	denotes	was
T2338	8877-8883	RB	denotes	highly
T2339	8884-8892	JJ	denotes	specific
T2340	8893-8899	IN	denotes	toward
T2341	8900-8903	DT	denotes	the
T2343	8904-8907	NN	denotes	HKI
T2342	8908-8915	NN	denotes	isoform
T2344	8915-8917	,	denotes	,
T2345	8917-8924	IN	denotes	because
T2347	8925-8929	NN	denotes	HKII
T2348	8929-8931	,	denotes	,
T2349	8931-8936	NN	denotes	HKIII
T2350	8936-8938	,	denotes	,
T2351	8938-8941	CC	denotes	and
T2352	8942-8953	NN	denotes	glucokinase
T2353	8954-8957	VBD	denotes	did
T2354	8958-8961	RB	denotes	not
T2346	8962-8970	VB	denotes	interact
T2355	8971-8975	IN	denotes	with
T2356	8976-8979	DT	denotes	the
T2357	8980-8982	NN	denotes	LD
T2358	8983-8985	IN	denotes	of
T2359	8986-8992	NN	denotes	RanBP2
T2360	8993-8994	-LRB-	denotes	(
T2362	8994-9005	JJ	denotes	unpublished
T2361	9006-9010	NNS	denotes	data
T2363	9010-9011	-RRB-	denotes	)
T2364	9011-9012	.	denotes	.
T2365	9012-9296	sentence	denotes	The interaction of Cox11, HKI, and mHsp70 with RanBP2 occurred in vivo in retinal extracts, since antibodies against these and RanBP2 coimmunoprecipitated RanBP2 (Figure 1E) and HKI (Figure 1F), respectively, and these interactions were observed across different tissues (Figure S1).
T2366	9013-9016	DT	denotes	The
T2367	9017-9028	NN	denotes	interaction
T2369	9029-9031	IN	denotes	of
T2370	9032-9037	NN	denotes	Cox11
T2371	9037-9039	,	denotes	,
T2372	9039-9042	NN	denotes	HKI
T2373	9042-9044	,	denotes	,
T2374	9044-9047	CC	denotes	and
T2375	9048-9054	NN	denotes	mHsp70
T2376	9055-9059	IN	denotes	with
T2377	9060-9066	NN	denotes	RanBP2
T2368	9067-9075	VBD	denotes	occurred
T2378	9076-9078	FW	denotes	in
T2379	9079-9083	FW	denotes	vivo
T2380	9084-9086	IN	denotes	in
T2381	9087-9094	JJ	denotes	retinal
T2382	9095-9103	NNS	denotes	extracts
T2383	9103-9105	,	denotes	,
T2384	9105-9110	IN	denotes	since
T2386	9111-9121	NNS	denotes	antibodies
T2387	9122-9129	IN	denotes	against
T2388	9130-9135	DT	denotes	these
T2390	9136-9139	CC	denotes	and
T2389	9140-9146	NN	denotes	RanBP2
T2385	9147-9167	VBD	denotes	coimmunoprecipitated
T2391	9168-9174	NN	denotes	RanBP2
T2392	9175-9176	-LRB-	denotes	(
T2394	9176-9182	NN	denotes	Figure
T2393	9183-9185	NN	denotes	1E
T2395	9185-9186	-RRB-	denotes	)
T2396	9187-9190	CC	denotes	and
T2397	9191-9194	NN	denotes	HKI
T2398	9195-9196	-LRB-	denotes	(
T2400	9196-9202	NN	denotes	Figure
T2399	9203-9205	NN	denotes	1F
T2401	9205-9206	-RRB-	denotes	)
T2402	9206-9208	,	denotes	,
T2403	9208-9220	RB	denotes	respectively
T2404	9220-9222	,	denotes	,
T2405	9222-9225	CC	denotes	and
T2406	9226-9231	DT	denotes	these
T2407	9232-9244	NNS	denotes	interactions
T2409	9245-9249	VBD	denotes	were
T2408	9250-9258	VBN	denotes	observed
T2410	9259-9265	IN	denotes	across
T2411	9266-9275	JJ	denotes	different
T2412	9276-9283	NNS	denotes	tissues
T2413	9284-9285	-LRB-	denotes	(
T2415	9285-9291	NN	denotes	Figure
T2414	9292-9294	NN	denotes	S1
T2416	9294-9295	-RRB-	denotes	)
T2417	9295-9296	.	denotes	.
T2418	9296-9486	sentence	denotes	Since RanBP2 exhibits chaperone activity, we assessed whether the interaction between the LD of RanBP2 and Cox11 was direct and the chaperone activity of LD toward folding species of Cox11.
T2419	9297-9302	IN	denotes	Since
T2421	9303-9309	NN	denotes	RanBP2
T2420	9310-9318	VBZ	denotes	exhibits
T2423	9319-9328	NN	denotes	chaperone
T2424	9329-9337	NN	denotes	activity
T2425	9337-9339	,	denotes	,
T2426	9339-9341	PRP	denotes	we
T2422	9342-9350	VBD	denotes	assessed
T2427	9351-9358	IN	denotes	whether
T2429	9359-9362	DT	denotes	the
T2430	9363-9374	NN	denotes	interaction
T2431	9375-9382	IN	denotes	between
T2432	9383-9386	DT	denotes	the
T2433	9387-9389	NN	denotes	LD
T2434	9390-9392	IN	denotes	of
T2435	9393-9399	NN	denotes	RanBP2
T2436	9400-9403	CC	denotes	and
T2437	9404-9409	NN	denotes	Cox11
T2428	9410-9413	VBD	denotes	was
T2438	9414-9420	JJ	denotes	direct
T2439	9421-9424	CC	denotes	and
T2441	9425-9428	DT	denotes	the
T2442	9429-9438	NN	denotes	chaperone
T2440	9439-9447	NN	denotes	activity
T2443	9448-9450	IN	denotes	of
T2444	9451-9453	NN	denotes	LD
T2445	9454-9460	IN	denotes	toward
T2446	9461-9468	VBG	denotes	folding
T2447	9469-9476	NNS	denotes	species
T2448	9477-9479	IN	denotes	of
T2449	9480-9485	NN	denotes	Cox11
T2450	9485-9486	.	denotes	.
T2451	9486-9666	sentence	denotes	Reconstitution binding assays were carried out between purified LD and Cox11, fully and partially denatured with GnHCl and urea, respectively, and native Cox11 (Figure 1G and 1H).
T2452	9487-9501	NN	denotes	Reconstitution
T2453	9502-9509	NN	denotes	binding
T2454	9510-9516	NNS	denotes	assays
T2456	9517-9521	VBD	denotes	were
T2455	9522-9529	VBN	denotes	carried
T2457	9530-9533	RP	denotes	out
T2458	9534-9541	IN	denotes	between
T2459	9542-9550	VBN	denotes	purified
T2460	9551-9553	NN	denotes	LD
T2461	9554-9557	CC	denotes	and
T2462	9558-9563	NN	denotes	Cox11
T2463	9563-9565	,	denotes	,
T2464	9565-9570	RB	denotes	fully
T2466	9571-9574	CC	denotes	and
T2467	9575-9584	RB	denotes	partially
T2465	9585-9594	VBN	denotes	denatured
T2468	9595-9599	IN	denotes	with
T2469	9600-9605	NN	denotes	GnHCl
T2470	9606-9609	CC	denotes	and
T2471	9610-9614	NN	denotes	urea
T2472	9614-9616	,	denotes	,
T2473	9616-9628	RB	denotes	respectively
T2474	9628-9630	,	denotes	,
T2475	9630-9633	CC	denotes	and
T2476	9634-9640	JJ	denotes	native
T2477	9641-9646	NN	denotes	Cox11
T2478	9647-9648	-LRB-	denotes	(
T2480	9648-9654	NN	denotes	Figure
T2479	9655-9657	NN	denotes	1G
T2481	9658-9661	CC	denotes	and
T2482	9662-9664	NN	denotes	1H
T2483	9664-9665	-RRB-	denotes	)
T2484	9665-9666	.	denotes	.
T2485	9666-9839	sentence	denotes	Partial denatured Cox11 exhibits significantly higher and concentration-dependent binding affinity toward LD compared with the native and fully denatured Cox11 (Figure 1G).
T2486	9667-9674	JJ	denotes	Partial
T2487	9675-9684	VBN	denotes	denatured
T2488	9685-9690	NN	denotes	Cox11
T2489	9691-9699	VBZ	denotes	exhibits
T2490	9700-9713	RB	denotes	significantly
T2491	9714-9720	JJR	denotes	higher
T2493	9721-9724	CC	denotes	and
T2494	9725-9738	NN	denotes	concentration
T2496	9738-9739	HYPH	denotes	-
T2495	9739-9748	JJ	denotes	dependent
T2497	9749-9756	NN	denotes	binding
T2492	9757-9765	NN	denotes	affinity
T2498	9766-9772	IN	denotes	toward
T2499	9773-9775	NN	denotes	LD
T2500	9776-9784	VBN	denotes	compared
T2501	9785-9789	IN	denotes	with
T2502	9790-9793	DT	denotes	the
T2504	9794-9800	JJ	denotes	native
T2505	9801-9804	CC	denotes	and
T2506	9805-9810	RB	denotes	fully
T2507	9811-9820	VBN	denotes	denatured
T2503	9821-9826	NN	denotes	Cox11
T2508	9827-9828	-LRB-	denotes	(
T2510	9828-9834	NN	denotes	Figure
T2509	9835-9837	NN	denotes	1G
T2511	9837-9838	-RRB-	denotes	)
T2512	9838-9839	.	denotes	.
T2513	9839-10076	sentence	denotes	In addition, native Cox11 purified upon expression in the presence of CuSO4 (a prosthetic group tightly bound to Cox11) [31], shows significantly higher binding activity toward the LD of RanBP2, than in the absence of CuSO4 (Figure 1H).
T2514	9840-9842	IN	denotes	In
T2516	9843-9851	NN	denotes	addition
T2517	9851-9853	,	denotes	,
T2518	9853-9859	JJ	denotes	native
T2519	9860-9865	NN	denotes	Cox11
T2520	9866-9874	VBN	denotes	purified
T2521	9875-9879	IN	denotes	upon
T2522	9880-9890	NN	denotes	expression
T2523	9891-9893	IN	denotes	in
T2524	9894-9897	DT	denotes	the
T2525	9898-9906	NN	denotes	presence
T2526	9907-9909	IN	denotes	of
T2527	9910-9915	NN	denotes	CuSO4
T2528	9916-9917	-LRB-	denotes	(
T2529	9917-9918	DT	denotes	a
T2531	9919-9929	JJ	denotes	prosthetic
T2530	9930-9935	NN	denotes	group
T2532	9936-9943	RB	denotes	tightly
T2533	9944-9949	VBN	denotes	bound
T2534	9950-9952	IN	denotes	to
T2535	9953-9958	NN	denotes	Cox11
T2536	9958-9959	-RRB-	denotes	)
T2537	9960-9961	-LRB-	denotes	[
T2538	9961-9963	CD	denotes	31
T2539	9963-9964	-RRB-	denotes	]
T2540	9964-9966	,	denotes	,
T2515	9966-9971	VBZ	denotes	shows
T2541	9972-9985	RB	denotes	significantly
T2542	9986-9992	JJR	denotes	higher
T2544	9993-10000	NN	denotes	binding
T2543	10001-10009	NN	denotes	activity
T2545	10010-10016	IN	denotes	toward
T2546	10017-10020	DT	denotes	the
T2547	10021-10023	NN	denotes	LD
T2548	10024-10026	IN	denotes	of
T2549	10027-10033	NN	denotes	RanBP2
T2550	10033-10035	,	denotes	,
T2551	10035-10039	IN	denotes	than
T2552	10040-10042	IN	denotes	in
T2553	10043-10046	DT	denotes	the
T2554	10047-10054	NN	denotes	absence
T2555	10055-10057	IN	denotes	of
T2556	10058-10063	NN	denotes	CuSO4
T2557	10064-10065	-LRB-	denotes	(
T2559	10065-10071	NN	denotes	Figure
T2558	10072-10074	NN	denotes	1H
T2560	10074-10075	-RRB-	denotes	)
T2561	10075-10076	.	denotes	.
T10852	10087-10090	DT	denotes	The
T10853	10091-10093	NN	denotes	LD
T10855	10094-10096	IN	denotes	of
T10856	10097-10103	NN	denotes	RanBP2
T10854	10104-10113	VBZ	denotes	Interacts
T10857	10114-10118	IN	denotes	with
T10858	10119-10124	NN	denotes	Cox11
T10859	10125-10128	CC	denotes	and
T10860	10129-10132	NN	denotes	HKI
T10861	10132-10203	sentence	denotes	(A) Primary structure of RanBP2 and its structural/functional domains.
T10862	10133-10134	-LRB-	denotes	(
T10863	10134-10135	LS	denotes	A
T10865	10135-10136	-RRB-	denotes	)
T10866	10137-10144	JJ	denotes	Primary
T10864	10145-10154	NN	denotes	structure
T10867	10155-10157	IN	denotes	of
T10868	10158-10164	NN	denotes	RanBP2
T10869	10165-10168	CC	denotes	and
T10870	10169-10172	PRP$	denotes	its
T10872	10173-10183	JJ	denotes	structural
T10874	10183-10184	HYPH	denotes	/
T10873	10184-10194	JJ	denotes	functional
T10871	10195-10202	NNS	denotes	domains
T10875	10202-10203	.	denotes	.
T10876	10203-10246	sentence	denotes	The N-terminal LD of RanBP2 is underlined.
T10877	10204-10207	DT	denotes	The
T10879	10208-10209	NN	denotes	N
T10881	10209-10210	HYPH	denotes	-
T10880	10210-10218	JJ	denotes	terminal
T10878	10219-10221	NN	denotes	LD
T10883	10222-10224	IN	denotes	of
T10884	10225-10231	NN	denotes	RanBP2
T10885	10232-10234	VBZ	denotes	is
T10882	10235-10245	VBN	denotes	underlined
T10886	10245-10246	.	denotes	.
T10887	10246-10296	sentence	denotes	(B) Sequence alignment of murine and yeast Cox11.
T10888	10247-10248	-LRB-	denotes	(
T10889	10248-10249	LS	denotes	B
T10891	10249-10250	-RRB-	denotes	)
T10892	10251-10259	NN	denotes	Sequence
T10890	10260-10269	NN	denotes	alignment
T10893	10270-10272	IN	denotes	of
T10894	10273-10279	JJ	denotes	murine
T10896	10280-10283	CC	denotes	and
T10897	10284-10289	NN	denotes	yeast
T10895	10290-10295	NN	denotes	Cox11
T10898	10295-10296	.	denotes	.
T10899	10296-10360	sentence	denotes	The yeast Cox11 C- and N-terminal domains are poorly conserved.
T10900	10297-10300	DT	denotes	The
T10902	10301-10306	NN	denotes	yeast
T10903	10307-10312	NN	denotes	Cox11
T10904	10313-10314	NN	denotes	C
T10906	10314-10315	HYPH	denotes	-
T10907	10316-10319	CC	denotes	and
T10908	10320-10321	NN	denotes	N
T10909	10321-10322	HYPH	denotes	-
T10905	10322-10330	JJ	denotes	terminal
T10901	10331-10338	NNS	denotes	domains
T10911	10339-10342	VBP	denotes	are
T10912	10343-10349	RB	denotes	poorly
T10910	10350-10359	VBN	denotes	conserved
T10913	10359-10360	.	denotes	.
T10914	10360-10460	sentence	denotes	Arrow and solid line denote the predicted mitochondrial cleavage site and membrane-spanning domain.
T10915	10361-10366	NN	denotes	Arrow
T10917	10367-10370	CC	denotes	and
T10918	10371-10376	JJ	denotes	solid
T10919	10377-10381	NN	denotes	line
T10916	10382-10388	VBP	denotes	denote
T10920	10389-10392	DT	denotes	the
T10922	10393-10402	VBN	denotes	predicted
T10923	10403-10416	JJ	denotes	mitochondrial
T10924	10417-10425	NN	denotes	cleavage
T10921	10426-10430	NN	denotes	site
T10925	10431-10434	CC	denotes	and
T10926	10435-10443	NN	denotes	membrane
T10928	10443-10444	HYPH	denotes	-
T10927	10444-10452	VBG	denotes	spanning
T10929	10453-10459	NN	denotes	domain
T10930	10459-10460	.	denotes	.
T10931	10460-10592	sentence	denotes	The dotted and dashed lines above the aligned sequences represent, respectively, Cox11-N and Cox11-C constructs shown in Figure 1C.
T10932	10461-10464	DT	denotes	The
T10934	10465-10471	JJ	denotes	dotted
T10935	10472-10475	CC	denotes	and
T10936	10476-10482	JJ	denotes	dashed
T10933	10483-10488	NNS	denotes	lines
T10938	10489-10494	IN	denotes	above
T10939	10495-10498	DT	denotes	the
T10941	10499-10506	JJ	denotes	aligned
T10940	10507-10516	NNS	denotes	sequences
T10937	10517-10526	VBP	denotes	represent
T10942	10526-10528	,	denotes	,
T10943	10528-10540	RB	denotes	respectively
T10944	10540-10542	,	denotes	,
T10945	10542-10547	NN	denotes	Cox11
T10947	10547-10548	HYPH	denotes	-
T10946	10548-10549	NN	denotes	N
T10949	10550-10553	CC	denotes	and
T10950	10554-10559	NN	denotes	Cox11
T10952	10559-10560	HYPH	denotes	-
T10951	10560-10561	NN	denotes	C
T10948	10562-10572	NNS	denotes	constructs
T10953	10573-10578	VBN	denotes	shown
T10954	10579-10581	IN	denotes	in
T10955	10582-10588	NN	denotes	Figure
T10956	10589-10591	NN	denotes	1C
T10957	10591-10592	.	denotes	.
T10958	10592-10679	sentence	denotes	(C) Structure-function analysis of the interaction between the LD of RanBP2 and Cox11.
T10959	10593-10594	-LRB-	denotes	(
T10960	10594-10595	LS	denotes	C
T10962	10595-10596	-RRB-	denotes	)
T10963	10597-10606	NN	denotes	Structure
T10965	10606-10607	HYPH	denotes	-
T10964	10607-10615	NN	denotes	function
T10961	10616-10624	NN	denotes	analysis
T10966	10625-10627	IN	denotes	of
T10967	10628-10631	DT	denotes	the
T10968	10632-10643	NN	denotes	interaction
T10969	10644-10651	IN	denotes	between
T10970	10652-10655	DT	denotes	the
T10971	10656-10658	NN	denotes	LD
T10972	10659-10661	IN	denotes	of
T10973	10662-10668	NN	denotes	RanBP2
T10974	10669-10672	CC	denotes	and
T10975	10673-10678	NN	denotes	Cox11
T10976	10678-10679	.	denotes	.
T10977	10679-10806	sentence	denotes	Optimal interaction between the LD and Cox11 occurred in the presence of constructs comprising both the complete LD and Cox11.
T10978	10680-10687	JJ	denotes	Optimal
T10979	10688-10699	NN	denotes	interaction
T10981	10700-10707	IN	denotes	between
T10982	10708-10711	DT	denotes	the
T10983	10712-10714	NN	denotes	LD
T10984	10715-10718	CC	denotes	and
T10985	10719-10724	NN	denotes	Cox11
T10980	10725-10733	VBD	denotes	occurred
T10986	10734-10736	IN	denotes	in
T10987	10737-10740	DT	denotes	the
T10988	10741-10749	NN	denotes	presence
T10989	10750-10752	IN	denotes	of
T10990	10753-10763	NNS	denotes	constructs
T10991	10764-10774	VBG	denotes	comprising
T10992	10775-10779	CC	denotes	both
T10994	10780-10783	DT	denotes	the
T10995	10784-10792	JJ	denotes	complete
T10993	10793-10795	NN	denotes	LD
T10996	10796-10799	CC	denotes	and
T10997	10800-10805	NN	denotes	Cox11
T10998	10805-10806	.	denotes	.
T10999	10806-11058	sentence	denotes	Although removal of the cytosolic N-terminal (Cox11-C) significantly decreased the interaction with LD, the mitochondrial intermembrane domain of Cox11 (Cox11-C) together with the C-terminal half of LD (LD-C) retained most of the interaction activity.
T11000	10807-10815	IN	denotes	Although
T11002	10816-10823	NN	denotes	removal
T11003	10824-10826	IN	denotes	of
T11004	10827-10830	DT	denotes	the
T11006	10831-10840	JJ	denotes	cytosolic
T11005	10841-10842	NN	denotes	N
T11007	10842-10843	HYPH	denotes	-
T11008	10843-10851	JJ	denotes	terminal
T11009	10852-10853	-LRB-	denotes	(
T11010	10853-10858	NN	denotes	Cox11
T11012	10858-10859	HYPH	denotes	-
T11011	10859-10860	NN	denotes	C
T11013	10860-10861	-RRB-	denotes	)
T11014	10862-10875	RB	denotes	significantly
T11001	10876-10885	VBD	denotes	decreased
T11016	10886-10889	DT	denotes	the
T11017	10890-10901	NN	denotes	interaction
T11018	10902-10906	IN	denotes	with
T11019	10907-10909	NN	denotes	LD
T11020	10909-10911	,	denotes	,
T11021	10911-10914	DT	denotes	the
T11023	10915-10928	JJ	denotes	mitochondrial
T11024	10929-10942	NN	denotes	intermembrane
T11022	10943-10949	NN	denotes	domain
T11025	10950-10952	IN	denotes	of
T11026	10953-10958	NN	denotes	Cox11
T11027	10959-10960	-LRB-	denotes	(
T11028	10960-10965	NN	denotes	Cox11
T11030	10965-10966	HYPH	denotes	-
T11029	10966-10967	NN	denotes	C
T11031	10967-10968	-RRB-	denotes	)
T11032	10969-10977	RB	denotes	together
T11033	10978-10982	IN	denotes	with
T11034	10983-10986	DT	denotes	the
T11036	10987-10988	NN	denotes	C
T11038	10988-10989	HYPH	denotes	-
T11037	10989-10997	JJ	denotes	terminal
T11035	10998-11002	NN	denotes	half
T11039	11003-11005	IN	denotes	of
T11040	11006-11008	NN	denotes	LD
T11041	11009-11010	-LRB-	denotes	(
T11042	11010-11012	NN	denotes	LD
T11044	11012-11013	HYPH	denotes	-
T11043	11013-11014	NN	denotes	C
T11045	11014-11015	-RRB-	denotes	)
T11015	11016-11024	VBD	denotes	retained
T11046	11025-11029	JJS	denotes	most
T11047	11030-11032	IN	denotes	of
T11048	11033-11036	DT	denotes	the
T11050	11037-11048	NN	denotes	interaction
T11049	11049-11057	NN	denotes	activity
T11051	11057-11058	.	denotes	.
T11052	11058-11130	sentence	denotes	LD-N and LD-C ended and began with the leucine zipper domain of RanBP2.
T11053	11059-11061	NN	denotes	LD
T11055	11061-11062	HYPH	denotes	-
T11054	11062-11063	NN	denotes	N
T11057	11064-11067	CC	denotes	and
T11058	11068-11070	NN	denotes	LD
T11060	11070-11071	HYPH	denotes	-
T11059	11071-11072	NN	denotes	C
T11056	11073-11078	VBD	denotes	ended
T11061	11079-11082	CC	denotes	and
T11062	11083-11088	VBD	denotes	began
T11063	11089-11093	IN	denotes	with
T11064	11094-11097	DT	denotes	the
T11066	11098-11105	NN	denotes	leucine
T11067	11106-11112	NN	denotes	zipper
T11065	11113-11119	NN	denotes	domain
T11068	11120-11122	IN	denotes	of
T11069	11123-11129	NN	denotes	RanBP2
T11070	11129-11130	.	denotes	.
T11071	11130-11242	sentence	denotes	White and black bars denote β-galactosidase activity and growth rates in selective growth medium, respectively.
T11072	11131-11136	JJ	denotes	White
T11074	11137-11140	CC	denotes	and
T11075	11141-11146	JJ	denotes	black
T11073	11147-11151	NNS	denotes	bars
T11076	11152-11158	VBP	denotes	denote
T11077	11159-11160	NN	denotes	β
T11079	11160-11161	HYPH	denotes	-
T11078	11161-11174	NN	denotes	galactosidase
T11080	11175-11183	NN	denotes	activity
T11081	11184-11187	CC	denotes	and
T11082	11188-11194	NN	denotes	growth
T11083	11195-11200	NNS	denotes	rates
T11084	11201-11203	IN	denotes	in
T11085	11204-11213	JJ	denotes	selective
T11087	11214-11220	NN	denotes	growth
T11086	11221-11227	NN	denotes	medium
T11088	11227-11229	,	denotes	,
T11089	11229-11241	RB	denotes	respectively
T11090	11241-11242	.	denotes	.
T11091	11242-11288	sentence	denotes	Results shown represent the mean ± SD, n = 3.
T11092	11243-11250	NNS	denotes	Results
T11094	11251-11256	VBN	denotes	shown
T11093	11257-11266	VBP	denotes	represent
T11096	11267-11270	DT	denotes	the
T11098	11271-11275	NN	denotes	mean
T11099	11276-11277	SYM	denotes	±
T11097	11278-11280	NN	denotes	SD
T11100	11280-11282	,	denotes	,
T11101	11282-11283	NN	denotes	n
T11102	11284-11285	SYM	denotes	=
T11095	11286-11287	CD	denotes	3
T11103	11287-11288	.	denotes	.
T11104	11288-11387	sentence	denotes	(D) GST pull-down assays with the LD of RanBP2 and its leucine zipper domain and retinal extracts.
T11105	11289-11290	-LRB-	denotes	(
T11106	11290-11291	LS	denotes	D
T11108	11291-11292	-RRB-	denotes	)
T11109	11293-11296	NN	denotes	GST
T11110	11297-11301	VB	denotes	pull
T11111	11301-11302	HYPH	denotes	-
T11112	11302-11306	RP	denotes	down
T11107	11307-11313	NNS	denotes	assays
T11113	11314-11318	IN	denotes	with
T11114	11319-11322	DT	denotes	the
T11115	11323-11325	NN	denotes	LD
T11116	11326-11328	IN	denotes	of
T11117	11329-11335	NN	denotes	RanBP2
T11118	11336-11339	CC	denotes	and
T11119	11340-11343	PRP$	denotes	its
T11121	11344-11351	NN	denotes	leucine
T11122	11352-11358	NN	denotes	zipper
T11120	11359-11365	NN	denotes	domain
T11123	11366-11369	CC	denotes	and
T11124	11370-11377	JJ	denotes	retinal
T11125	11378-11386	NNS	denotes	extracts
T11126	11386-11387	.	denotes	.
T11127	11387-11513	sentence	denotes	The LD, but not the leucine zipper domain of RanBP2, associate with Cox11 (top panel, lane 1) and HKI (bottom panel, lane 1).
T11128	11388-11391	DT	denotes	The
T11129	11392-11394	NN	denotes	LD
T11131	11394-11396	,	denotes	,
T11132	11396-11399	CC	denotes	but
T11133	11400-11403	RB	denotes	not
T11134	11404-11407	DT	denotes	the
T11136	11408-11415	NN	denotes	leucine
T11137	11416-11422	NN	denotes	zipper
T11135	11423-11429	NN	denotes	domain
T11138	11430-11432	IN	denotes	of
T11139	11433-11439	NN	denotes	RanBP2
T11140	11439-11441	,	denotes	,
T11130	11441-11450	VBP	denotes	associate
T11141	11451-11455	IN	denotes	with
T11142	11456-11461	NN	denotes	Cox11
T11143	11462-11463	-LRB-	denotes	(
T11145	11463-11466	JJ	denotes	top
T11146	11467-11472	NN	denotes	panel
T11147	11472-11474	,	denotes	,
T11144	11474-11478	NN	denotes	lane
T11148	11479-11480	CD	denotes	1
T11149	11480-11481	-RRB-	denotes	)
T11150	11482-11485	CC	denotes	and
T11151	11486-11489	NN	denotes	HKI
T11152	11490-11491	-LRB-	denotes	(
T11154	11491-11497	JJ	denotes	bottom
T11155	11498-11503	NN	denotes	panel
T11156	11503-11505	,	denotes	,
T11153	11505-11509	NN	denotes	lane
T11157	11510-11511	CD	denotes	1
T11158	11511-11512	-RRB-	denotes	)
T11159	11512-11513	.	denotes	.
T11160	11513-11701	sentence	denotes	(E) Coimmunoprecipitation of RanBP2 with antibodies against its molecular partners shows that RanBP2 forms a complex in vivo with HKI (lanes 1 and 2), mHsp70 (lane 3), and Cox11 (lane 4).
T11161	11514-11515	-LRB-	denotes	(
T11162	11515-11516	LS	denotes	E
T11164	11516-11517	-RRB-	denotes	)
T11165	11518-11539	NN	denotes	Coimmunoprecipitation
T11166	11540-11542	IN	denotes	of
T11167	11543-11549	NN	denotes	RanBP2
T11168	11550-11554	IN	denotes	with
T11169	11555-11565	NNS	denotes	antibodies
T11170	11566-11573	IN	denotes	against
T11171	11574-11577	PRP$	denotes	its
T11173	11578-11587	JJ	denotes	molecular
T11172	11588-11596	NNS	denotes	partners
T11163	11597-11602	VBZ	denotes	shows
T11174	11603-11607	IN	denotes	that
T11176	11608-11614	NN	denotes	RanBP2
T11175	11615-11620	VBZ	denotes	forms
T11177	11621-11622	DT	denotes	a
T11178	11623-11630	JJ	denotes	complex
T11179	11631-11633	FW	denotes	in
T11180	11634-11638	FW	denotes	vivo
T11181	11639-11643	IN	denotes	with
T11182	11644-11647	NN	denotes	HKI
T11183	11648-11649	-LRB-	denotes	(
T11185	11649-11654	NNS	denotes	lanes
T11184	11655-11656	CD	denotes	1
T11186	11657-11660	CC	denotes	and
T11187	11661-11662	CD	denotes	2
T11188	11662-11663	-RRB-	denotes	)
T11189	11663-11665	,	denotes	,
T11190	11665-11671	NN	denotes	mHsp70
T11191	11672-11673	-LRB-	denotes	(
T11192	11673-11677	NN	denotes	lane
T11193	11678-11679	CD	denotes	3
T11194	11679-11680	-RRB-	denotes	)
T11195	11680-11682	,	denotes	,
T11196	11682-11685	CC	denotes	and
T11197	11686-11691	NN	denotes	Cox11
T11198	11692-11693	-LRB-	denotes	(
T11199	11693-11697	NN	denotes	lane
T11200	11698-11699	CD	denotes	4
T11201	11699-11700	-RRB-	denotes	)
T11202	11700-11701	.	denotes	.
T11203	11701-11853	sentence	denotes	Lanes 5, 6, and 7 are control immunoprecipitation reactions with different antibodies against the RanBP2 domains, KBD, ZnF, and XAFXFG of nucleoporins.
T11204	11702-11707	NNS	denotes	Lanes
T11205	11708-11709	CD	denotes	5
T11207	11709-11711	,	denotes	,
T11208	11711-11712	CD	denotes	6
T11209	11712-11714	,	denotes	,
T11210	11714-11717	CC	denotes	and
T11211	11718-11719	CD	denotes	7
T11206	11720-11723	VBP	denotes	are
T11212	11724-11731	NN	denotes	control
T11214	11732-11751	NN	denotes	immunoprecipitation
T11213	11752-11761	NNS	denotes	reactions
T11215	11762-11766	IN	denotes	with
T11216	11767-11776	JJ	denotes	different
T11217	11777-11787	NNS	denotes	antibodies
T11218	11788-11795	IN	denotes	against
T11219	11796-11799	DT	denotes	the
T11221	11800-11806	NN	denotes	RanBP2
T11220	11807-11814	NNS	denotes	domains
T11222	11814-11816	,	denotes	,
T11223	11816-11819	NN	denotes	KBD
T11224	11819-11821	,	denotes	,
T11225	11821-11824	NN	denotes	ZnF
T11226	11824-11826	,	denotes	,
T11227	11826-11829	CC	denotes	and
T11228	11830-11836	NN	denotes	XAFXFG
T11229	11837-11839	IN	denotes	of
T11230	11840-11852	NNS	denotes	nucleoporins
T11231	11852-11853	.	denotes	.
T11232	11853-11953	sentence	denotes	(F) Reciprocal coimmunoprecipitation of HKI with antibodies against RanBP2 (used and shown in (E)).
T11233	11854-11855	-LRB-	denotes	(
T11234	11855-11856	LS	denotes	F
T11236	11856-11857	-RRB-	denotes	)
T11237	11858-11868	JJ	denotes	Reciprocal
T11235	11869-11890	NN	denotes	coimmunoprecipitation
T11238	11891-11893	IN	denotes	of
T11239	11894-11897	NN	denotes	HKI
T11240	11898-11902	IN	denotes	with
T11241	11903-11913	NNS	denotes	antibodies
T11242	11914-11921	IN	denotes	against
T11243	11922-11928	NN	denotes	RanBP2
T11244	11929-11930	-LRB-	denotes	(
T11245	11930-11934	VBN	denotes	used
T11246	11935-11938	CC	denotes	and
T11247	11939-11944	VBN	denotes	shown
T11248	11945-11947	IN	denotes	in
T11249	11948-11949	-LRB-	denotes	(
T11250	11949-11950	NN	denotes	E
T11251	11950-11951	-RRB-	denotes	)
T11252	11951-11952	-RRB-	denotes	)
T11253	11952-11953	.	denotes	.
T11254	11953-12230	sentence	denotes	(G) Reconstitution pull-down assays with purified LD and increasing concentrations of native (top panel), denatured (middle panel), and partially denatured (bottom panel) Cox11, respectively, in the absence and presence of denaturating agent, GnHCl and chaotropic agent, urea.
T11255	11954-11955	-LRB-	denotes	(
T11256	11955-11956	LS	denotes	G
T11258	11956-11957	-RRB-	denotes	)
T11259	11958-11972	NN	denotes	Reconstitution
T11260	11973-11977	VB	denotes	pull
T11261	11977-11978	HYPH	denotes	-
T11262	11978-11982	RP	denotes	down
T11257	11983-11989	NNS	denotes	assays
T11263	11990-11994	IN	denotes	with
T11264	11995-12003	VBN	denotes	purified
T11265	12004-12006	NN	denotes	LD
T11266	12007-12010	CC	denotes	and
T11267	12011-12021	VBG	denotes	increasing
T11268	12022-12036	NNS	denotes	concentrations
T11269	12037-12039	IN	denotes	of
T11270	12040-12046	JJ	denotes	native
T11272	12047-12048	-LRB-	denotes	(
T11274	12048-12051	JJ	denotes	top
T11273	12052-12057	NN	denotes	panel
T11275	12057-12058	-RRB-	denotes	)
T11276	12058-12060	,	denotes	,
T11277	12060-12069	VBN	denotes	denatured
T11278	12070-12071	-LRB-	denotes	(
T11280	12071-12077	JJ	denotes	middle
T11279	12078-12083	NN	denotes	panel
T11281	12083-12084	-RRB-	denotes	)
T11282	12084-12086	,	denotes	,
T11283	12086-12089	CC	denotes	and
T11284	12090-12099	RB	denotes	partially
T11285	12100-12109	VBN	denotes	denatured
T11286	12110-12111	-LRB-	denotes	(
T11288	12111-12117	JJ	denotes	bottom
T11287	12118-12123	NN	denotes	panel
T11289	12123-12124	-RRB-	denotes	)
T11271	12125-12130	NN	denotes	Cox11
T11290	12130-12132	,	denotes	,
T11291	12132-12144	RB	denotes	respectively
T11292	12144-12146	,	denotes	,
T11293	12146-12148	IN	denotes	in
T11294	12149-12152	DT	denotes	the
T11295	12153-12160	NN	denotes	absence
T11296	12161-12164	CC	denotes	and
T11297	12165-12173	NN	denotes	presence
T11298	12174-12176	IN	denotes	of
T11299	12177-12189	VBG	denotes	denaturating
T11300	12190-12195	NN	denotes	agent
T11301	12195-12197	,	denotes	,
T11302	12197-12202	NN	denotes	GnHCl
T11303	12203-12206	CC	denotes	and
T11304	12207-12217	JJ	denotes	chaotropic
T11305	12218-12223	NN	denotes	agent
T11306	12223-12225	,	denotes	,
T11307	12225-12229	NN	denotes	urea
T11308	12229-12230	.	denotes	.
T11309	12230-12337	sentence	denotes	Folding intermediates (lower panel) of Cox11 exhibit the highest binding activity toward the LD of RanBP2.
T11310	12231-12238	NN	denotes	Folding
T11311	12239-12252	NNS	denotes	intermediates
T11313	12253-12254	-LRB-	denotes	(
T11315	12254-12259	JJR	denotes	lower
T11314	12260-12265	NN	denotes	panel
T11316	12265-12266	-RRB-	denotes	)
T11317	12267-12269	IN	denotes	of
T11318	12270-12275	NN	denotes	Cox11
T11312	12276-12283	VBP	denotes	exhibit
T11319	12284-12287	DT	denotes	the
T11321	12288-12295	JJS	denotes	highest
T11322	12296-12303	NN	denotes	binding
T11320	12304-12312	NN	denotes	activity
T11323	12313-12319	IN	denotes	toward
T11324	12320-12323	DT	denotes	the
T11325	12324-12326	NN	denotes	LD
T11326	12327-12329	IN	denotes	of
T11327	12330-12336	NN	denotes	RanBP2
T11328	12336-12337	.	denotes	.
T11329	12337-12481	sentence	denotes	(H) Similar experiments as in (G) but in the presence of native Cox11 expressed in the absence (top panel) or presence (bottom panel) of CuSO4.
T11330	12338-12339	-LRB-	denotes	(
T11331	12339-12340	LS	denotes	H
T11333	12340-12341	-RRB-	denotes	)
T11334	12342-12349	JJ	denotes	Similar
T11332	12350-12361	NNS	denotes	experiments
T11335	12362-12364	IN	denotes	as
T11336	12365-12367	IN	denotes	in
T11337	12368-12369	-LRB-	denotes	(
T11338	12369-12370	NN	denotes	G
T11339	12370-12371	-RRB-	denotes	)
T11340	12372-12375	IN	denotes	but
T11341	12376-12378	IN	denotes	in
T11342	12379-12382	DT	denotes	the
T11343	12383-12391	NN	denotes	presence
T11344	12392-12394	IN	denotes	of
T11345	12395-12401	JJ	denotes	native
T11346	12402-12407	NN	denotes	Cox11
T11347	12408-12417	VBN	denotes	expressed
T11348	12418-12420	IN	denotes	in
T11349	12421-12424	DT	denotes	the
T11350	12425-12432	NN	denotes	absence
T11351	12433-12434	-LRB-	denotes	(
T11353	12434-12437	JJ	denotes	top
T11352	12438-12443	NN	denotes	panel
T11354	12443-12444	-RRB-	denotes	)
T11355	12445-12447	CC	denotes	or
T11356	12448-12456	NN	denotes	presence
T11357	12457-12458	-LRB-	denotes	(
T11359	12458-12464	JJ	denotes	bottom
T11358	12465-12470	NN	denotes	panel
T11360	12470-12471	-RRB-	denotes	)
T11361	12472-12474	IN	denotes	of
T11362	12475-12480	NN	denotes	CuSO4
T11363	12480-12481	.	denotes	.
T11364	12481-12575	sentence	denotes	The mature isoform of the metallochaperone has an increased affinity toward the LD of RanBP2.
T11365	12482-12485	DT	denotes	The
T11367	12486-12492	JJ	denotes	mature
T11366	12493-12500	NN	denotes	isoform
T11369	12501-12503	IN	denotes	of
T11370	12504-12507	DT	denotes	the
T11371	12508-12524	NN	denotes	metallochaperone
T11368	12525-12528	VBZ	denotes	has
T11372	12529-12531	DT	denotes	an
T11374	12532-12541	VBN	denotes	increased
T11373	12542-12550	NN	denotes	affinity
T11375	12551-12557	IN	denotes	toward
T11376	12558-12561	DT	denotes	the
T11377	12562-12564	NN	denotes	LD
T11378	12565-12567	IN	denotes	of
T11379	12568-12574	NN	denotes	RanBP2
T11380	12574-12575	.	denotes	.
T11381	12575-12818	sentence	denotes	LD, leucine-rich domain; LZ, leucine zipper domain; RBD1–4, Ran-binding domains 1–4; ZnF, zinc finger cluster domain; KBD, kinesin (KIF5B/KIF5C)-binding domain; CLD, cyclophilin-like domain; IR, internal repeat domain; CY, cyclophilin domain.
T11382	12576-12578	NN	denotes	LD
T11383	12578-12580	,	denotes	,
T11384	12580-12587	NN	denotes	leucine
T11386	12587-12588	HYPH	denotes	-
T11385	12588-12592	JJ	denotes	rich
T11387	12593-12599	NN	denotes	domain
T11388	12599-12600	:	denotes	;
T11389	12601-12603	NN	denotes	LZ
T11390	12603-12605	,	denotes	,
T11391	12605-12612	NN	denotes	leucine
T11392	12613-12619	NN	denotes	zipper
T11393	12620-12626	NN	denotes	domain
T11394	12626-12627	:	denotes	;
T11395	12628-12632	NN	denotes	RBD1
T11396	12632-12633	SYM	denotes	–
T11397	12633-12634	CD	denotes	4
T11398	12634-12636	,	denotes	,
T11399	12636-12639	NN	denotes	Ran
T11401	12639-12640	HYPH	denotes	-
T11400	12640-12647	NN	denotes	binding
T11402	12648-12655	NNS	denotes	domains
T11403	12656-12657	CD	denotes	1
T11405	12657-12658	SYM	denotes	–
T11404	12658-12659	CD	denotes	4
T11406	12659-12660	:	denotes	;
T11407	12661-12664	NN	denotes	ZnF
T11408	12664-12666	,	denotes	,
T11409	12666-12670	NN	denotes	zinc
T11410	12671-12677	NN	denotes	finger
T11412	12678-12685	NN	denotes	cluster
T11411	12686-12692	NN	denotes	domain
T11413	12692-12693	:	denotes	;
T11414	12694-12697	NN	denotes	KBD
T11415	12697-12699	,	denotes	,
T11416	12699-12706	NN	denotes	kinesin
T11418	12707-12708	-LRB-	denotes	(
T11419	12708-12713	NN	denotes	KIF5B
T11421	12713-12714	HYPH	denotes	/
T11420	12714-12719	NN	denotes	KIF5C
T11422	12719-12720	-RRB-	denotes	)
T11423	12720-12721	HYPH	denotes	-
T11417	12721-12728	NN	denotes	binding
T11424	12729-12735	NN	denotes	domain
T11425	12735-12736	:	denotes	;
T11426	12737-12740	NN	denotes	CLD
T11427	12740-12742	,	denotes	,
T11428	12742-12753	NN	denotes	cyclophilin
T11430	12753-12754	HYPH	denotes	-
T11429	12754-12758	JJ	denotes	like
T11431	12759-12765	NN	denotes	domain
T11432	12765-12766	:	denotes	;
T11433	12767-12769	NN	denotes	IR
T11434	12769-12771	,	denotes	,
T11435	12771-12779	JJ	denotes	internal
T11436	12780-12786	NN	denotes	repeat
T11437	12787-12793	NN	denotes	domain
T11438	12793-12794	:	denotes	;
T11439	12795-12797	NN	denotes	CY
T11440	12797-12799	,	denotes	,
T11441	12799-12810	NN	denotes	cyclophilin
T11442	12811-12817	NN	denotes	domain
T11443	12817-12818	.	denotes	.
T2637	12820-12823	NN	denotes	Cox
T2639	12824-12826	CD	denotes	11
T2638	12827-12835	VBZ	denotes	Inhibits
T2640	12836-12839	NN	denotes	HKI
T2641	12840-12848	NN	denotes	Activity
T2642	12849-12852	CC	denotes	and
T2643	12853-12856	DT	denotes	the
T2644	12857-12859	NN	denotes	LD
T2645	12860-12862	IN	denotes	of
T2646	12863-12869	NN	denotes	RanBP2
T2647	12870-12878	VBZ	denotes	Reverses
T2648	12879-12882	DT	denotes	the
T2649	12883-12893	NN	denotes	Inhibition
T2650	12894-12896	IN	denotes	of
T2651	12897-12902	NN	denotes	Cox11
T2652	12903-12907	IN	denotes	over
T2653	12908-12911	NN	denotes	HKI
T2654	12911-13151	sentence	denotes	To probe whether the interaction of Cox11 and HKI with the LD of RanBP2 modulates the enzymatic activity of HKI, we first examined the effect of increasing concentrations of Cox11 on the initial rates of HKI enzymatic activity (Figure 2A).
T2655	12912-12914	TO	denotes	To
T2656	12915-12920	VB	denotes	probe
T2658	12921-12928	IN	denotes	whether
T2660	12929-12932	DT	denotes	the
T2661	12933-12944	NN	denotes	interaction
T2662	12945-12947	IN	denotes	of
T2663	12948-12953	NN	denotes	Cox11
T2664	12954-12957	CC	denotes	and
T2665	12958-12961	NN	denotes	HKI
T2666	12962-12966	IN	denotes	with
T2667	12967-12970	DT	denotes	the
T2668	12971-12973	NN	denotes	LD
T2669	12974-12976	IN	denotes	of
T2670	12977-12983	NN	denotes	RanBP2
T2659	12984-12993	VBZ	denotes	modulates
T2671	12994-12997	DT	denotes	the
T2673	12998-13007	JJ	denotes	enzymatic
T2672	13008-13016	NN	denotes	activity
T2674	13017-13019	IN	denotes	of
T2675	13020-13023	NN	denotes	HKI
T2676	13023-13025	,	denotes	,
T2677	13025-13027	PRP	denotes	we
T2678	13028-13033	RB	denotes	first
T2657	13034-13042	VBD	denotes	examined
T2679	13043-13046	DT	denotes	the
T2680	13047-13053	NN	denotes	effect
T2681	13054-13056	IN	denotes	of
T2682	13057-13067	VBG	denotes	increasing
T2683	13068-13082	NNS	denotes	concentrations
T2684	13083-13085	IN	denotes	of
T2685	13086-13091	NN	denotes	Cox11
T2686	13092-13094	IN	denotes	on
T2687	13095-13098	DT	denotes	the
T2689	13099-13106	JJ	denotes	initial
T2688	13107-13112	NNS	denotes	rates
T2690	13113-13115	IN	denotes	of
T2691	13116-13119	NN	denotes	HKI
T2693	13120-13129	JJ	denotes	enzymatic
T2692	13130-13138	NN	denotes	activity
T2694	13139-13140	-LRB-	denotes	(
T2696	13140-13146	NN	denotes	Figure
T2695	13147-13149	NN	denotes	2A
T2697	13149-13150	-RRB-	denotes	)
T2698	13150-13151	.	denotes	.
T2699	13151-13298	sentence	denotes	Cox11 strongly inhibits HKI activity in a concentration-dependent fashion, and at ~15 nM of Cox11, HKI activity could not be recorded (Figure 2A).
T2700	13152-13157	NN	denotes	Cox11
T2702	13158-13166	RB	denotes	strongly
T2701	13167-13175	VBZ	denotes	inhibits
T2703	13176-13179	NN	denotes	HKI
T2704	13180-13188	NN	denotes	activity
T2705	13189-13191	IN	denotes	in
T2706	13192-13193	DT	denotes	a
T2708	13194-13207	NN	denotes	concentration
T2710	13207-13208	HYPH	denotes	-
T2709	13208-13217	JJ	denotes	dependent
T2707	13218-13225	NN	denotes	fashion
T2711	13225-13227	,	denotes	,
T2712	13227-13230	CC	denotes	and
T2713	13231-13233	IN	denotes	at
T2715	13234-13235	SYM	denotes	~
T2716	13235-13237	CD	denotes	15
T2717	13238-13240	NN	denotes	nM
T2718	13241-13243	IN	denotes	of
T2719	13244-13249	NN	denotes	Cox11
T2720	13249-13251	,	denotes	,
T2721	13251-13254	NN	denotes	HKI
T2722	13255-13263	NN	denotes	activity
T2723	13264-13269	MD	denotes	could
T2724	13270-13273	RB	denotes	not
T2725	13274-13276	VB	denotes	be
T2714	13277-13285	VBN	denotes	recorded
T2726	13286-13287	-LRB-	denotes	(
T2728	13287-13293	NN	denotes	Figure
T2727	13294-13296	NN	denotes	2A
T2729	13296-13297	-RRB-	denotes	)
T2730	13297-13298	.	denotes	.
T2731	13298-13412	sentence	denotes	Cox11 behaves as a partial noncompetitive inhibitor of HKI by affecting the V max of HKI for glucose (Figure 2B).
T2732	13299-13304	NN	denotes	Cox11
T2733	13305-13312	VBZ	denotes	behaves
T2734	13313-13315	IN	denotes	as
T2735	13316-13317	DT	denotes	a
T2737	13318-13325	JJ	denotes	partial
T2738	13326-13340	JJ	denotes	noncompetitive
T2736	13341-13350	NN	denotes	inhibitor
T2739	13351-13353	IN	denotes	of
T2740	13354-13357	NN	denotes	HKI
T2741	13358-13360	IN	denotes	by
T2742	13361-13370	VBG	denotes	affecting
T2743	13371-13374	DT	denotes	the
T2744	13375-13380	NN	denotes	V max
T2745	13381-13383	IN	denotes	of
T2746	13384-13387	NN	denotes	HKI
T2747	13388-13391	IN	denotes	for
T2748	13392-13399	NN	denotes	glucose
T2749	13400-13401	-LRB-	denotes	(
T2751	13401-13407	NN	denotes	Figure
T2750	13408-13410	NN	denotes	2B
T2752	13410-13411	-RRB-	denotes	)
T2753	13411-13412	.	denotes	.
T2754	13412-13627	sentence	denotes	Then, we evaluated the effect of the LD of RanBP2 on the HK activity in the presence of a fixed inhibitory concentration of Cox11, saturating concentration of glucose substrate, and increasing concentrations of LD.
T2755	13413-13417	RB	denotes	Then
T2757	13417-13419	,	denotes	,
T2758	13419-13421	PRP	denotes	we
T2756	13422-13431	VBD	denotes	evaluated
T2759	13432-13435	DT	denotes	the
T2760	13436-13442	NN	denotes	effect
T2761	13443-13445	IN	denotes	of
T2762	13446-13449	DT	denotes	the
T2763	13450-13452	NN	denotes	LD
T2764	13453-13455	IN	denotes	of
T2765	13456-13462	NN	denotes	RanBP2
T2766	13463-13465	IN	denotes	on
T2767	13466-13469	DT	denotes	the
T2769	13470-13472	NN	denotes	HK
T2768	13473-13481	NN	denotes	activity
T2770	13482-13484	IN	denotes	in
T2771	13485-13488	DT	denotes	the
T2772	13489-13497	NN	denotes	presence
T2773	13498-13500	IN	denotes	of
T2774	13501-13502	DT	denotes	a
T2776	13503-13508	VBN	denotes	fixed
T2777	13509-13519	JJ	denotes	inhibitory
T2775	13520-13533	NN	denotes	concentration
T2778	13534-13536	IN	denotes	of
T2779	13537-13542	NN	denotes	Cox11
T2780	13542-13544	,	denotes	,
T2781	13544-13554	VBG	denotes	saturating
T2782	13555-13568	NN	denotes	concentration
T2783	13569-13571	IN	denotes	of
T2784	13572-13579	NN	denotes	glucose
T2785	13580-13589	NN	denotes	substrate
T2786	13589-13591	,	denotes	,
T2787	13591-13594	CC	denotes	and
T2788	13595-13605	VBG	denotes	increasing
T2789	13606-13620	NNS	denotes	concentrations
T2790	13621-13623	IN	denotes	of
T2791	13624-13626	NN	denotes	LD
T2792	13626-13627	.	denotes	.
T2793	13627-14001	sentence	denotes	As shown in Figure 2C, the LD domain sharply reversed the inhibitory effect of Cox11 on HKI activity in a concentration-dependent manner, but under saturating (and stochiometric) amounts of LD, the velocity of the reaction did not reach that observed for HKI activity in the absence of Cox11 (Figure 2A), suggesting the LD by itself may also have an effect on HKI activity.
T2794	13628-13630	IN	denotes	As
T2795	13631-13636	VBN	denotes	shown
T2797	13637-13639	IN	denotes	in
T2798	13640-13646	NN	denotes	Figure
T2799	13647-13649	NN	denotes	2C
T2800	13649-13651	,	denotes	,
T2801	13651-13654	DT	denotes	the
T2803	13655-13657	NN	denotes	LD
T2802	13658-13664	NN	denotes	domain
T2804	13665-13672	RB	denotes	sharply
T2796	13673-13681	VBD	denotes	reversed
T2805	13682-13685	DT	denotes	the
T2807	13686-13696	JJ	denotes	inhibitory
T2806	13697-13703	NN	denotes	effect
T2808	13704-13706	IN	denotes	of
T2809	13707-13712	NN	denotes	Cox11
T2810	13713-13715	IN	denotes	on
T2811	13716-13719	NN	denotes	HKI
T2812	13720-13728	NN	denotes	activity
T2813	13729-13731	IN	denotes	in
T2814	13732-13733	DT	denotes	a
T2816	13734-13747	NN	denotes	concentration
T2818	13747-13748	HYPH	denotes	-
T2817	13748-13757	JJ	denotes	dependent
T2815	13758-13764	NN	denotes	manner
T2819	13764-13766	,	denotes	,
T2820	13766-13769	CC	denotes	but
T2821	13770-13775	IN	denotes	under
T2823	13776-13786	VBG	denotes	saturating
T2825	13787-13788	-LRB-	denotes	(
T2826	13788-13791	CC	denotes	and
T2827	13792-13805	JJ	denotes	stochiometric
T2828	13805-13806	-RRB-	denotes	)
T2824	13807-13814	NNS	denotes	amounts
T2829	13815-13817	IN	denotes	of
T2830	13818-13820	NN	denotes	LD
T2831	13820-13822	,	denotes	,
T2832	13822-13825	DT	denotes	the
T2833	13826-13834	NN	denotes	velocity
T2834	13835-13837	IN	denotes	of
T2835	13838-13841	DT	denotes	the
T2836	13842-13850	NN	denotes	reaction
T2837	13851-13854	VBD	denotes	did
T2838	13855-13858	RB	denotes	not
T2822	13859-13864	VB	denotes	reach
T2839	13865-13869	DT	denotes	that
T2840	13870-13878	VBN	denotes	observed
T2841	13879-13882	IN	denotes	for
T2842	13883-13886	NN	denotes	HKI
T2843	13887-13895	NN	denotes	activity
T2844	13896-13898	IN	denotes	in
T2845	13899-13902	DT	denotes	the
T2846	13903-13910	NN	denotes	absence
T2847	13911-13913	IN	denotes	of
T2848	13914-13919	NN	denotes	Cox11
T2849	13920-13921	-LRB-	denotes	(
T2851	13921-13927	NN	denotes	Figure
T2850	13928-13930	NN	denotes	2A
T2852	13930-13931	-RRB-	denotes	)
T2853	13931-13933	,	denotes	,
T2854	13933-13943	VBG	denotes	suggesting
T2855	13944-13947	DT	denotes	the
T2856	13948-13950	NN	denotes	LD
T2858	13951-13953	IN	denotes	by
T2859	13954-13960	PRP	denotes	itself
T2860	13961-13964	MD	denotes	may
T2861	13965-13969	RB	denotes	also
T2857	13970-13974	VB	denotes	have
T2862	13975-13977	DT	denotes	an
T2863	13978-13984	NN	denotes	effect
T2864	13985-13987	IN	denotes	on
T2865	13988-13991	NN	denotes	HKI
T2866	13992-14000	NN	denotes	activity
T2867	14000-14001	.	denotes	.
T2868	14001-14129	sentence	denotes	Indeed, a saturating concentration of LD reduced the V max but not the K m of HKI (Figure 2D) by ~20% under similar conditions.
T2869	14002-14008	RB	denotes	Indeed
T2871	14008-14010	,	denotes	,
T2872	14010-14011	DT	denotes	a
T2874	14012-14022	NN	denotes	saturating
T2873	14023-14036	NN	denotes	concentration
T2875	14037-14039	IN	denotes	of
T2876	14040-14042	NN	denotes	LD
T2870	14043-14050	VBD	denotes	reduced
T2877	14051-14054	DT	denotes	the
T2878	14055-14060	NN	denotes	V max
T2879	14061-14064	CC	denotes	but
T2880	14065-14068	RB	denotes	not
T2881	14069-14072	DT	denotes	the
T2882	14073-14076	NN	denotes	K m
T2883	14077-14079	IN	denotes	of
T2884	14080-14083	NN	denotes	HKI
T2885	14084-14085	-LRB-	denotes	(
T2887	14085-14091	NN	denotes	Figure
T2886	14092-14094	NN	denotes	2D
T2888	14094-14095	-RRB-	denotes	)
T2889	14096-14098	IN	denotes	by
T2890	14099-14100	SYM	denotes	~
T2891	14100-14102	CD	denotes	20
T2892	14102-14103	NN	denotes	%
T2893	14104-14109	IN	denotes	under
T2894	14110-14117	JJ	denotes	similar
T2895	14118-14128	NNS	denotes	conditions
T2896	14128-14129	.	denotes	.
T11520	14140-14146	NN	denotes	Effect
T11521	14147-14149	IN	denotes	of
T11522	14150-14155	NN	denotes	Cox11
T11523	14156-14159	CC	denotes	and
T11524	14160-14166	NN	denotes	RanBP2
T11525	14167-14169	IN	denotes	on
T11526	14170-14173	NN	denotes	HKI
T11527	14174-14182	NN	denotes	Activity
T11528	14182-14347	sentence	denotes	(A) Saturation kinetics, rate versus glucose of HKI (0.24 μg) in the absence (solid circles) and presence of Cox11 (open circles, 0.25 nM; solid triangles, 7.5 nM).
T11529	14183-14184	-LRB-	denotes	(
T11530	14184-14185	LS	denotes	A
T11532	14185-14186	-RRB-	denotes	)
T11533	14187-14197	NN	denotes	Saturation
T11531	14198-14206	NNS	denotes	kinetics
T11534	14206-14208	,	denotes	,
T11535	14208-14212	NN	denotes	rate
T11536	14213-14219	CC	denotes	versus
T11537	14220-14227	NN	denotes	glucose
T11538	14228-14230	IN	denotes	of
T11539	14231-14234	NN	denotes	HKI
T11540	14235-14236	-LRB-	denotes	(
T11542	14236-14240	CD	denotes	0.24
T11541	14241-14243	NNS	denotes	μg
T11543	14243-14244	-RRB-	denotes	)
T11544	14245-14247	IN	denotes	in
T11545	14248-14251	DT	denotes	the
T11546	14252-14259	NN	denotes	absence
T11547	14260-14261	-LRB-	denotes	(
T11549	14261-14266	JJ	denotes	solid
T11548	14267-14274	NNS	denotes	circles
T11550	14274-14275	-RRB-	denotes	)
T11551	14276-14279	CC	denotes	and
T11552	14280-14288	NN	denotes	presence
T11553	14289-14291	IN	denotes	of
T11554	14292-14297	NN	denotes	Cox11
T11555	14298-14299	-LRB-	denotes	(
T11557	14299-14303	JJ	denotes	open
T11558	14304-14311	NNS	denotes	circles
T11560	14311-14313	,	denotes	,
T11561	14313-14317	CD	denotes	0.25
T11559	14318-14320	NNS	denotes	nM
T11562	14320-14321	:	denotes	;
T11563	14322-14327	JJ	denotes	solid
T11564	14328-14337	NNS	denotes	triangles
T11565	14337-14339	,	denotes	,
T11566	14339-14342	CD	denotes	7.5
T11556	14343-14345	NNS	denotes	nM
T11567	14345-14346	-RRB-	denotes	)
T11568	14346-14347	.	denotes	.
T11569	14347-14418	sentence	denotes	The activity of HKI decreases with increasing concentrations of Cox11.
T11570	14348-14351	DT	denotes	The
T11571	14352-14360	NN	denotes	activity
T11573	14361-14363	IN	denotes	of
T11574	14364-14367	NN	denotes	HKI
T11572	14368-14377	VBZ	denotes	decreases
T11575	14378-14382	IN	denotes	with
T11576	14383-14393	VBG	denotes	increasing
T11577	14394-14408	NNS	denotes	concentrations
T11578	14409-14411	IN	denotes	of
T11579	14412-14417	NN	denotes	Cox11
T11580	14417-14418	.	denotes	.
T11581	14418-14512	sentence	denotes	No measurable HKI activity was recorded in the presence of 15 nM of Cox11 (unpublished data).
T11582	14419-14421	DT	denotes	No
T11584	14422-14432	JJ	denotes	measurable
T11585	14433-14436	NN	denotes	HKI
T11583	14437-14445	NN	denotes	activity
T11587	14446-14449	VBD	denotes	was
T11586	14450-14458	VBN	denotes	recorded
T11588	14459-14461	IN	denotes	in
T11589	14462-14465	DT	denotes	the
T11590	14466-14474	NN	denotes	presence
T11591	14475-14477	IN	denotes	of
T11592	14478-14480	CD	denotes	15
T11593	14481-14483	NNS	denotes	nM
T11594	14484-14486	IN	denotes	of
T11595	14487-14492	NN	denotes	Cox11
T11596	14493-14494	-LRB-	denotes	(
T11598	14494-14505	JJ	denotes	unpublished
T11597	14506-14510	NNS	denotes	data
T11599	14510-14511	-RRB-	denotes	)
T11600	14511-14512	.	denotes	.
T11601	14512-14625	sentence	denotes	(B) Hanes-Wolf plot of (A) (1/rate versus glucose) in the absence and presence of fixed concentrations of Cox11.
T11602	14513-14514	-LRB-	denotes	(
T11603	14514-14515	LS	denotes	B
T11605	14515-14516	-RRB-	denotes	)
T11606	14517-14522	NNP	denotes	Hanes
T11608	14522-14523	HYPH	denotes	-
T11607	14523-14527	NNP	denotes	Wolf
T11604	14528-14532	NN	denotes	plot
T11609	14533-14535	IN	denotes	of
T11610	14536-14537	-LRB-	denotes	(
T11611	14537-14538	NN	denotes	A
T11612	14538-14539	-RRB-	denotes	)
T11613	14540-14541	-LRB-	denotes	(
T11614	14541-14542	CD	denotes	1
T11615	14542-14543	SYM	denotes	/
T11616	14543-14547	NN	denotes	rate
T11617	14548-14554	CC	denotes	versus
T11618	14555-14562	NN	denotes	glucose
T11619	14562-14563	-RRB-	denotes	)
T11620	14564-14566	IN	denotes	in
T11621	14567-14570	DT	denotes	the
T11622	14571-14578	NN	denotes	absence
T11623	14579-14582	CC	denotes	and
T11624	14583-14591	NN	denotes	presence
T11625	14592-14594	IN	denotes	of
T11626	14595-14600	VBN	denotes	fixed
T11627	14601-14615	NNS	denotes	concentrations
T11628	14616-14618	IN	denotes	of
T11629	14619-14624	NN	denotes	Cox11
T11630	14624-14625	.	denotes	.
T11631	14625-14731	sentence	denotes	Linearity of reciprocal plots also supported the hyperbolic behavior of the reactions (unpublished data).
T11632	14626-14635	NN	denotes	Linearity
T11634	14636-14638	IN	denotes	of
T11635	14639-14649	JJ	denotes	reciprocal
T11636	14650-14655	NNS	denotes	plots
T11637	14656-14660	RB	denotes	also
T11633	14661-14670	VBD	denotes	supported
T11638	14671-14674	DT	denotes	the
T11640	14675-14685	JJ	denotes	hyperbolic
T11639	14686-14694	NN	denotes	behavior
T11641	14695-14697	IN	denotes	of
T11642	14698-14701	DT	denotes	the
T11643	14702-14711	NNS	denotes	reactions
T11644	14712-14713	-LRB-	denotes	(
T11646	14713-14724	JJ	denotes	unpublished
T11645	14725-14729	NNS	denotes	data
T11647	14729-14730	-RRB-	denotes	)
T11648	14730-14731	.	denotes	.
T11649	14731-14843	sentence	denotes	Cox11 behaves as a noncompetitive inhibitor of HKI by reducing the V max of HKI but not its K m toward glucose.
T11650	14732-14737	NN	denotes	Cox11
T11651	14738-14745	VBZ	denotes	behaves
T11652	14746-14748	IN	denotes	as
T11653	14749-14750	DT	denotes	a
T11655	14751-14765	JJ	denotes	noncompetitive
T11654	14766-14775	NN	denotes	inhibitor
T11656	14776-14778	IN	denotes	of
T11657	14779-14782	NN	denotes	HKI
T11658	14783-14785	IN	denotes	by
T11659	14786-14794	VBG	denotes	reducing
T11660	14795-14798	DT	denotes	the
T11661	14799-14804	NN	denotes	V max
T11662	14805-14807	IN	denotes	of
T11663	14808-14811	NN	denotes	HKI
T11664	14812-14815	CC	denotes	but
T11665	14816-14819	RB	denotes	not
T11666	14820-14823	PRP$	denotes	its
T11667	14824-14827	NN	denotes	K m
T11668	14828-14834	IN	denotes	toward
T11669	14835-14842	NN	denotes	glucose
T11670	14842-14843	.	denotes	.
T11671	14843-14964	sentence	denotes	(C) HKI rate is plotted as a function of LD concentration at saturating glucose and fixed Cox11 (7.5 nM) concentrations.
T11672	14844-14845	-LRB-	denotes	(
T11673	14845-14846	LS	denotes	C
T11675	14846-14847	-RRB-	denotes	)
T11676	14848-14851	NN	denotes	HKI
T11677	14852-14856	NN	denotes	rate
T11678	14857-14859	VBZ	denotes	is
T11674	14860-14867	VBN	denotes	plotted
T11679	14868-14870	IN	denotes	as
T11680	14871-14872	DT	denotes	a
T11681	14873-14881	NN	denotes	function
T11682	14882-14884	IN	denotes	of
T11683	14885-14887	NN	denotes	LD
T11684	14888-14901	NN	denotes	concentration
T11685	14902-14904	IN	denotes	at
T11686	14905-14915	VBG	denotes	saturating
T11687	14916-14923	NN	denotes	glucose
T11689	14924-14927	CC	denotes	and
T11690	14928-14933	VBN	denotes	fixed
T11691	14934-14939	NN	denotes	Cox11
T11692	14940-14941	-LRB-	denotes	(
T11694	14941-14944	CD	denotes	7.5
T11693	14945-14947	NNS	denotes	nM
T11695	14947-14948	-RRB-	denotes	)
T11688	14949-14963	NNS	denotes	concentrations
T11696	14963-14964	.	denotes	.
T11697	14964-15069	sentence	denotes	Note that increasing concentrations of the LD of RanBP2 reverse the inhibition of HKI activity by Cox11.
T11698	14965-14969	VB	denotes	Note
T11699	14970-14974	IN	denotes	that
T11701	14975-14985	VBG	denotes	increasing
T11702	14986-15000	NNS	denotes	concentrations
T11703	15001-15003	IN	denotes	of
T11704	15004-15007	DT	denotes	the
T11705	15008-15010	NN	denotes	LD
T11706	15011-15013	IN	denotes	of
T11707	15014-15020	NN	denotes	RanBP2
T11700	15021-15028	VBP	denotes	reverse
T11708	15029-15032	DT	denotes	the
T11709	15033-15043	NN	denotes	inhibition
T11710	15044-15046	IN	denotes	of
T11711	15047-15050	NN	denotes	HKI
T11712	15051-15059	NN	denotes	activity
T11713	15060-15062	IN	denotes	by
T11714	15063-15068	NN	denotes	Cox11
T11715	15068-15069	.	denotes	.
T11716	15069-15213	sentence	denotes	A half-maximal effect of the LD of RanBP2 on HKI activity in the presence of 7.5 nM of Cox11 was observed at a concentration of ~0.05 nM of LD.
T11717	15070-15071	DT	denotes	A
T11719	15072-15076	RB	denotes	half
T11721	15076-15077	HYPH	denotes	-
T11720	15077-15084	JJ	denotes	maximal
T11718	15085-15091	NN	denotes	effect
T11723	15092-15094	IN	denotes	of
T11724	15095-15098	DT	denotes	the
T11725	15099-15101	NN	denotes	LD
T11726	15102-15104	IN	denotes	of
T11727	15105-15111	NN	denotes	RanBP2
T11728	15112-15114	IN	denotes	on
T11729	15115-15118	NN	denotes	HKI
T11730	15119-15127	NN	denotes	activity
T11731	15128-15130	IN	denotes	in
T11732	15131-15134	DT	denotes	the
T11733	15135-15143	NN	denotes	presence
T11734	15144-15146	IN	denotes	of
T11735	15147-15150	CD	denotes	7.5
T11736	15151-15153	NNS	denotes	nM
T11737	15154-15156	IN	denotes	of
T11738	15157-15162	NN	denotes	Cox11
T11739	15163-15166	VBD	denotes	was
T11722	15167-15175	VBN	denotes	observed
T11740	15176-15178	IN	denotes	at
T11741	15179-15180	DT	denotes	a
T11742	15181-15194	NN	denotes	concentration
T11743	15195-15197	IN	denotes	of
T11744	15198-15199	SYM	denotes	~
T11745	15199-15203	CD	denotes	0.05
T11746	15204-15206	NNS	denotes	nM
T11747	15207-15209	IN	denotes	of
T11748	15210-15212	NN	denotes	LD
T11749	15212-15213	.	denotes	.
T11750	15213-15291	sentence	denotes	(D) Rate versus glucose plot in the absence and presence of the LD of RanBP2.
T11751	15214-15215	-LRB-	denotes	(
T11752	15215-15216	LS	denotes	D
T11754	15216-15217	-RRB-	denotes	)
T11755	15218-15222	NN	denotes	Rate
T11756	15223-15229	CC	denotes	versus
T11757	15230-15237	NN	denotes	glucose
T11753	15238-15242	NN	denotes	plot
T11758	15243-15245	IN	denotes	in
T11759	15246-15249	DT	denotes	the
T11760	15250-15257	NN	denotes	absence
T11761	15258-15261	CC	denotes	and
T11762	15262-15270	NN	denotes	presence
T11763	15271-15273	IN	denotes	of
T11764	15274-15277	DT	denotes	the
T11765	15278-15280	NN	denotes	LD
T11766	15281-15283	IN	denotes	of
T11767	15284-15290	NN	denotes	RanBP2
T11768	15290-15291	.	denotes	.
T11769	15291-15395	sentence	denotes	At a saturating concentration of the LD of RanBP2 (3.75 nM), the HKI activity was reduced by about 20%.
T11770	15292-15294	IN	denotes	At
T11772	15295-15296	DT	denotes	a
T11774	15297-15307	NN	denotes	saturating
T11773	15308-15321	NN	denotes	concentration
T11775	15322-15324	IN	denotes	of
T11776	15325-15328	DT	denotes	the
T11777	15329-15331	NN	denotes	LD
T11778	15332-15334	IN	denotes	of
T11779	15335-15341	NN	denotes	RanBP2
T11780	15342-15343	-LRB-	denotes	(
T11782	15343-15347	CD	denotes	3.75
T11781	15348-15350	NNS	denotes	nM
T11783	15350-15351	-RRB-	denotes	)
T11784	15351-15353	,	denotes	,
T11785	15353-15356	DT	denotes	the
T11787	15357-15360	NN	denotes	HKI
T11786	15361-15369	NN	denotes	activity
T11788	15370-15373	VBD	denotes	was
T11771	15374-15381	VBN	denotes	reduced
T11789	15382-15384	IN	denotes	by
T11790	15385-15390	IN	denotes	about
T11791	15391-15393	CD	denotes	20
T11792	15393-15394	NN	denotes	%
T11793	15394-15395	.	denotes	.
T11794	15395-15416	sentence	denotes	v, rate; S, glucose.
T11795	15396-15397	NN	denotes	v
T11796	15397-15399	,	denotes	,
T11797	15399-15403	NN	denotes	rate
T11798	15403-15404	:	denotes	;
T11799	15405-15406	NN	denotes	S
T11800	15406-15408	,	denotes	,
T11801	15408-15415	NN	denotes	glucose
T11802	15415-15416	.	denotes	.
T2996	15418-15424	NN	denotes	RanBP2
T2997	15425-15436	VBZ	denotes	Colocalizes
T2998	15437-15441	IN	denotes	with
T2999	15442-15445	NN	denotes	HK1
T3000	15445-15447	,	denotes	,
T3001	15447-15452	NN	denotes	Cox11
T3002	15452-15454	,	denotes	,
T3003	15454-15457	CC	denotes	and
T3004	15458-15464	NN	denotes	mHsp70
T3005	15465-15467	IN	denotes	in
T3006	15468-15471	DT	denotes	the
T3007	15472-15478	NN	denotes	Retina
T3008	15479-15482	CC	denotes	and
T3009	15483-15491	VBN	denotes	Cultured
T3010	15492-15499	NNS	denotes	Neurons
T3011	15499-15737	sentence	denotes	In addition to its presence at the vicinity of NPCs, we have shown previously that RanBP2 is localized to and abundant in the mitochondria-rich ellipsoid subcellular compartment of photosensory (photoreceptor) neurons of the retina [19].
T3012	15500-15502	IN	denotes	In
T3014	15503-15511	NN	denotes	addition
T3015	15512-15514	IN	denotes	to
T3016	15515-15518	PRP$	denotes	its
T3017	15519-15527	NN	denotes	presence
T3018	15528-15530	IN	denotes	at
T3019	15531-15534	DT	denotes	the
T3020	15535-15543	NN	denotes	vicinity
T3021	15544-15546	IN	denotes	of
T3022	15547-15551	NNS	denotes	NPCs
T3023	15551-15553	,	denotes	,
T3024	15553-15555	PRP	denotes	we
T3025	15556-15560	VBP	denotes	have
T3013	15561-15566	VBN	denotes	shown
T3026	15567-15577	RB	denotes	previously
T3027	15578-15582	IN	denotes	that
T3029	15583-15589	NN	denotes	RanBP2
T3028	15590-15592	VBZ	denotes	is
T3030	15593-15602	VBN	denotes	localized
T3031	15603-15605	IN	denotes	to
T3032	15606-15609	CC	denotes	and
T3033	15610-15618	JJ	denotes	abundant
T3035	15619-15621	IN	denotes	in
T3036	15622-15625	DT	denotes	the
T3037	15626-15638	NNS	denotes	mitochondria
T3039	15638-15639	HYPH	denotes	-
T3038	15639-15643	JJ	denotes	rich
T3040	15644-15653	JJ	denotes	ellipsoid
T3041	15654-15665	JJ	denotes	subcellular
T3034	15666-15677	NN	denotes	compartment
T3042	15678-15680	IN	denotes	of
T3043	15681-15693	NN	denotes	photosensory
T3045	15694-15695	-LRB-	denotes	(
T3046	15695-15708	NN	denotes	photoreceptor
T3047	15708-15709	-RRB-	denotes	)
T3044	15710-15717	NNS	denotes	neurons
T3048	15718-15720	IN	denotes	of
T3049	15721-15724	DT	denotes	the
T3050	15725-15731	NN	denotes	retina
T3051	15732-15733	-LRB-	denotes	[
T3052	15733-15735	CD	denotes	19
T3053	15735-15736	-RRB-	denotes	]
T3054	15736-15737	.	denotes	.
T3055	15737-16170	sentence	denotes	We extended the subcellular colocalization studies on RanBP2 and its novel partners by immunocytochemistry to determine if RanBP2, Cox11, HKI, and mHsp70 colocalize in hippocampal neurons (Figure 3A–3C), cerebral cortex neurons (Figure 3D–3F), ellipsoid (mitochondria-rich) subcellular compartments of photosensory neurons of the retina (Figure 3G–3O), and dissociated primary glia and neuron cultures from the brain (Figure 3P–3Z).
T3056	15738-15740	PRP	denotes	We
T3057	15741-15749	VBD	denotes	extended
T3058	15750-15753	DT	denotes	the
T3060	15754-15765	JJ	denotes	subcellular
T3061	15766-15780	NN	denotes	colocalization
T3059	15781-15788	NNS	denotes	studies
T3062	15789-15791	IN	denotes	on
T3063	15792-15798	NN	denotes	RanBP2
T3064	15799-15802	CC	denotes	and
T3065	15803-15806	PRP$	denotes	its
T3067	15807-15812	JJ	denotes	novel
T3066	15813-15821	NNS	denotes	partners
T3068	15822-15824	IN	denotes	by
T3069	15825-15844	NN	denotes	immunocytochemistry
T3070	15845-15847	TO	denotes	to
T3071	15848-15857	VB	denotes	determine
T3072	15858-15860	IN	denotes	if
T3074	15861-15867	NN	denotes	RanBP2
T3075	15867-15869	,	denotes	,
T3076	15869-15874	NN	denotes	Cox11
T3077	15874-15876	,	denotes	,
T3078	15876-15879	NN	denotes	HKI
T3079	15879-15881	,	denotes	,
T3080	15881-15884	CC	denotes	and
T3081	15885-15891	NN	denotes	mHsp70
T3073	15892-15902	VBP	denotes	colocalize
T3082	15903-15905	IN	denotes	in
T3083	15906-15917	JJ	denotes	hippocampal
T3084	15918-15925	NNS	denotes	neurons
T3085	15926-15927	-LRB-	denotes	(
T3087	15927-15933	NN	denotes	Figure
T3086	15934-15936	NN	denotes	3A
T3088	15936-15937	SYM	denotes	–
T3089	15937-15939	NN	denotes	3C
T3090	15939-15940	-RRB-	denotes	)
T3091	15940-15942	,	denotes	,
T3092	15942-15950	JJ	denotes	cerebral
T3093	15951-15957	NN	denotes	cortex
T3094	15958-15965	NNS	denotes	neurons
T3095	15966-15967	-LRB-	denotes	(
T3097	15967-15973	NN	denotes	Figure
T3096	15974-15976	NN	denotes	3D
T3098	15976-15977	SYM	denotes	–
T3099	15977-15979	NN	denotes	3F
T3100	15979-15980	-RRB-	denotes	)
T3101	15980-15982	,	denotes	,
T3102	15982-15991	JJ	denotes	ellipsoid
T3104	15992-15993	-LRB-	denotes	(
T3105	15993-16005	NNS	denotes	mitochondria
T3107	16005-16006	HYPH	denotes	-
T3106	16006-16010	JJ	denotes	rich
T3108	16010-16011	-RRB-	denotes	)
T3109	16012-16023	JJ	denotes	subcellular
T3103	16024-16036	NNS	denotes	compartments
T3110	16037-16039	IN	denotes	of
T3111	16040-16052	JJ	denotes	photosensory
T3112	16053-16060	NNS	denotes	neurons
T3113	16061-16063	IN	denotes	of
T3114	16064-16067	DT	denotes	the
T3115	16068-16074	NN	denotes	retina
T3116	16075-16076	-LRB-	denotes	(
T3118	16076-16082	NN	denotes	Figure
T3117	16083-16085	NN	denotes	3G
T3119	16085-16086	SYM	denotes	–
T3120	16086-16088	NN	denotes	3O
T3121	16088-16089	-RRB-	denotes	)
T3122	16089-16091	,	denotes	,
T3123	16091-16094	CC	denotes	and
T3124	16095-16106	VBN	denotes	dissociated
T3126	16107-16114	JJ	denotes	primary
T3125	16115-16119	NN	denotes	glia
T3127	16120-16123	CC	denotes	and
T3128	16124-16130	NN	denotes	neuron
T3129	16131-16139	NNS	denotes	cultures
T3130	16140-16144	IN	denotes	from
T3131	16145-16148	DT	denotes	the
T3132	16149-16154	NN	denotes	brain
T3133	16155-16156	-LRB-	denotes	(
T3135	16156-16162	NN	denotes	Figure
T3134	16163-16165	NN	denotes	3P
T3136	16165-16166	SYM	denotes	–
T3137	16166-16168	NN	denotes	3Z
T3138	16168-16169	-RRB-	denotes	)
T3139	16169-16170	.	denotes	.
T3140	16170-16295	sentence	denotes	Double immunostaining with antibodies against these proteins showed that they colocalize to the mitochondria (Figure 3A–3Z).
T3141	16171-16177	JJ	denotes	Double
T3142	16178-16192	NN	denotes	immunostaining
T3144	16193-16197	IN	denotes	with
T3145	16198-16208	NNS	denotes	antibodies
T3146	16209-16216	IN	denotes	against
T3147	16217-16222	DT	denotes	these
T3148	16223-16231	NN	denotes	proteins
T3143	16232-16238	VBD	denotes	showed
T3149	16239-16243	IN	denotes	that
T3151	16244-16248	PRP	denotes	they
T3150	16249-16259	VBP	denotes	colocalize
T3152	16260-16262	IN	denotes	to
T3153	16263-16266	DT	denotes	the
T3154	16267-16279	NNS	denotes	mitochondria
T3155	16280-16281	-LRB-	denotes	(
T3157	16281-16287	NN	denotes	Figure
T3156	16288-16290	NN	denotes	3A
T3158	16290-16291	SYM	denotes	–
T3159	16291-16293	NN	denotes	3Z
T3160	16293-16294	-RRB-	denotes	)
T3161	16294-16295	.	denotes	.
T11945	16306-16318	NN	denotes	Localization
T11946	16319-16321	IN	denotes	of
T11947	16322-16328	NN	denotes	RanBP2
T11948	16329-16332	CC	denotes	and
T11949	16333-16336	PRP$	denotes	Its
T11951	16337-16339	NN	denotes	LD
T11952	16340-16349	JJ	denotes	Molecular
T11950	16350-16358	NNS	denotes	Partners
T11953	16358-16556	sentence	denotes	(A–F) are thin cryosections of an area of the hipocampus (CA1 neurons) and cerebral cortex, respectively, immunostained against HKI (A and D), RanBP2 (B and E), and merged images thereof (C and F).
T11954	16359-16360	-LRB-	denotes	(
T11956	16360-16361	NN	denotes	A
T11957	16361-16362	SYM	denotes	–
T11958	16362-16363	NN	denotes	F
T11959	16363-16364	-RRB-	denotes	)
T11955	16365-16368	VBP	denotes	are
T11960	16369-16373	JJ	denotes	thin
T11961	16374-16386	NNS	denotes	cryosections
T11962	16387-16389	IN	denotes	of
T11963	16390-16392	DT	denotes	an
T11964	16393-16397	NN	denotes	area
T11965	16398-16400	IN	denotes	of
T11966	16401-16404	DT	denotes	the
T11967	16405-16415	NN	denotes	hipocampus
T11968	16416-16417	-LRB-	denotes	(
T11970	16417-16420	NN	denotes	CA1
T11969	16421-16428	NNS	denotes	neurons
T11971	16428-16429	-RRB-	denotes	)
T11972	16430-16433	CC	denotes	and
T11973	16434-16442	JJ	denotes	cerebral
T11974	16443-16449	NN	denotes	cortex
T11975	16449-16451	,	denotes	,
T11976	16451-16463	RB	denotes	respectively
T11977	16463-16465	,	denotes	,
T11978	16465-16478	VBN	denotes	immunostained
T11979	16479-16486	IN	denotes	against
T11980	16487-16490	NN	denotes	HKI
T11981	16491-16492	-LRB-	denotes	(
T11982	16492-16493	NN	denotes	A
T11983	16494-16497	CC	denotes	and
T11984	16498-16499	NN	denotes	D
T11985	16499-16500	-RRB-	denotes	)
T11986	16500-16502	,	denotes	,
T11987	16502-16508	NN	denotes	RanBP2
T11988	16509-16510	-LRB-	denotes	(
T11989	16510-16511	NN	denotes	B
T11990	16512-16515	CC	denotes	and
T11991	16516-16517	NN	denotes	E
T11992	16517-16518	-RRB-	denotes	)
T11993	16518-16520	,	denotes	,
T11994	16520-16523	CC	denotes	and
T11995	16524-16530	VBN	denotes	merged
T11996	16531-16537	NNS	denotes	images
T11997	16538-16545	RB	denotes	thereof
T11998	16546-16547	-LRB-	denotes	(
T11999	16547-16548	NN	denotes	C
T12000	16549-16552	CC	denotes	and
T12001	16553-16554	NN	denotes	F
T12002	16554-16555	-RRB-	denotes	)
T12003	16555-16556	.	denotes	.
T12004	16556-16774	sentence	denotes	Note that while RanBP2 and HKI are widely expressed among and colocalize to hippocampal neurons (C), HKI expression and localization with RanBP2 is restricted to a subset of cortical neurons (likely interneurons) (F).
T12005	16557-16561	VB	denotes	Note
T12006	16562-16566	IN	denotes	that
T12008	16567-16572	IN	denotes	while
T12010	16573-16579	NN	denotes	RanBP2
T12011	16580-16583	CC	denotes	and
T12012	16584-16587	NN	denotes	HKI
T12013	16588-16591	VBP	denotes	are
T12014	16592-16598	RB	denotes	widely
T12009	16599-16608	VBN	denotes	expressed
T12015	16609-16614	IN	denotes	among
T12016	16615-16618	CC	denotes	and
T12017	16619-16629	VBP	denotes	colocalize
T12018	16630-16632	IN	denotes	to
T12019	16633-16644	JJ	denotes	hippocampal
T12020	16645-16652	NNS	denotes	neurons
T12021	16653-16654	-LRB-	denotes	(
T12022	16654-16655	NN	denotes	C
T12023	16655-16656	-RRB-	denotes	)
T12024	16656-16658	,	denotes	,
T12025	16658-16661	NN	denotes	HKI
T12026	16662-16672	NN	denotes	expression
T12027	16673-16676	CC	denotes	and
T12028	16677-16689	NN	denotes	localization
T12029	16690-16694	IN	denotes	with
T12030	16695-16701	NN	denotes	RanBP2
T12031	16702-16704	VBZ	denotes	is
T12007	16705-16715	VBN	denotes	restricted
T12032	16716-16718	IN	denotes	to
T12033	16719-16720	DT	denotes	a
T12034	16721-16727	NN	denotes	subset
T12035	16728-16730	IN	denotes	of
T12036	16731-16739	JJ	denotes	cortical
T12037	16740-16747	NNS	denotes	neurons
T12038	16748-16749	-LRB-	denotes	(
T12039	16749-16755	JJ	denotes	likely
T12040	16756-16768	NNS	denotes	interneurons
T12041	16768-16769	-RRB-	denotes	)
T12042	16770-16771	-LRB-	denotes	(
T12043	16771-16772	NN	denotes	F
T12044	16772-16773	-RRB-	denotes	)
T12045	16773-16774	.	denotes	.
T12046	16774-17094	sentence	denotes	Images of the distal region of bovine retinal cryosections comprising part of the nuclear layer of photoreceptor neurons and their inner (myoid and ellipsoid) segment compartment (G–O) are immunostained against mHsp70 (G) and RanBP2 (H), mHsp70 (J) and Cox11 (K), HKI (M) and Cox11 (N), and merged images thereof (I–O).
T12047	16775-16781	NNS	denotes	Images
T12049	16782-16784	IN	denotes	of
T12050	16785-16788	DT	denotes	the
T12052	16789-16795	JJ	denotes	distal
T12051	16796-16802	NN	denotes	region
T12053	16803-16805	IN	denotes	of
T12054	16806-16812	JJ	denotes	bovine
T12056	16813-16820	JJ	denotes	retinal
T12055	16821-16833	NNS	denotes	cryosections
T12057	16834-16844	VBG	denotes	comprising
T12058	16845-16849	NN	denotes	part
T12059	16850-16852	IN	denotes	of
T12060	16853-16856	DT	denotes	the
T12062	16857-16864	JJ	denotes	nuclear
T12061	16865-16870	NN	denotes	layer
T12063	16871-16873	IN	denotes	of
T12064	16874-16887	NN	denotes	photoreceptor
T12065	16888-16895	NNS	denotes	neurons
T12066	16896-16899	CC	denotes	and
T12067	16900-16905	PRP$	denotes	their
T12069	16906-16911	JJ	denotes	inner
T12070	16912-16913	-LRB-	denotes	(
T12071	16913-16918	JJ	denotes	myoid
T12072	16919-16922	CC	denotes	and
T12073	16923-16932	JJ	denotes	ellipsoid
T12074	16932-16933	-RRB-	denotes	)
T12075	16934-16941	NN	denotes	segment
T12068	16942-16953	NN	denotes	compartment
T12076	16954-16955	-LRB-	denotes	(
T12077	16955-16956	NN	denotes	G
T12078	16956-16957	SYM	denotes	–
T12079	16957-16958	NN	denotes	O
T12080	16958-16959	-RRB-	denotes	)
T12081	16960-16963	VBP	denotes	are
T12048	16964-16977	VBN	denotes	immunostained
T12082	16978-16985	IN	denotes	against
T12083	16986-16992	NN	denotes	mHsp70
T12084	16993-16994	-LRB-	denotes	(
T12085	16994-16995	NN	denotes	G
T12086	16995-16996	-RRB-	denotes	)
T12087	16997-17000	CC	denotes	and
T12088	17001-17007	NN	denotes	RanBP2
T12089	17008-17009	-LRB-	denotes	(
T12090	17009-17010	NN	denotes	H
T12091	17010-17011	-RRB-	denotes	)
T12092	17011-17013	,	denotes	,
T12093	17013-17019	NN	denotes	mHsp70
T12094	17020-17021	-LRB-	denotes	(
T12095	17021-17022	NN	denotes	J
T12096	17022-17023	-RRB-	denotes	)
T12097	17024-17027	CC	denotes	and
T12098	17028-17033	NN	denotes	Cox11
T12099	17034-17035	-LRB-	denotes	(
T12100	17035-17036	NN	denotes	K
T12101	17036-17037	-RRB-	denotes	)
T12102	17037-17039	,	denotes	,
T12103	17039-17042	NN	denotes	HKI
T12104	17043-17044	-LRB-	denotes	(
T12105	17044-17045	NN	denotes	M
T12106	17045-17046	-RRB-	denotes	)
T12107	17047-17050	CC	denotes	and
T12108	17051-17056	NN	denotes	Cox11
T12109	17057-17058	-LRB-	denotes	(
T12110	17058-17059	NN	denotes	N
T12111	17059-17060	-RRB-	denotes	)
T12112	17060-17062	,	denotes	,
T12113	17062-17065	CC	denotes	and
T12114	17066-17072	VBN	denotes	merged
T12115	17073-17079	NNS	denotes	images
T12116	17080-17087	RB	denotes	thereof
T12117	17088-17089	-LRB-	denotes	(
T12118	17089-17090	NN	denotes	I
T12119	17090-17091	SYM	denotes	–
T12120	17091-17092	NN	denotes	O
T12121	17092-17093	-RRB-	denotes	)
T12122	17093-17094	.	denotes	.
T12123	17094-17370	sentence	denotes	Note the prominent localization of RanBP2, mHsp70, and Cox11 at the mitochondria-rich ellipsoid compartment of photoreceptors and the colocalization of RanBP2 and Cox11 with mHsp70 (I and L), while HKI colocalization with Cox11 was limited to restricted foci (R, arrowheads).
T12124	17095-17099	VB	denotes	Note
T12125	17100-17103	DT	denotes	the
T12127	17104-17113	JJ	denotes	prominent
T12126	17114-17126	NN	denotes	localization
T12128	17127-17129	IN	denotes	of
T12129	17130-17136	NN	denotes	RanBP2
T12130	17136-17138	,	denotes	,
T12131	17138-17144	NN	denotes	mHsp70
T12132	17144-17146	,	denotes	,
T12133	17146-17149	CC	denotes	and
T12134	17150-17155	NN	denotes	Cox11
T12135	17156-17158	IN	denotes	at
T12136	17159-17162	DT	denotes	the
T12138	17163-17175	NNS	denotes	mitochondria
T12140	17175-17176	HYPH	denotes	-
T12139	17176-17180	JJ	denotes	rich
T12141	17181-17190	JJ	denotes	ellipsoid
T12137	17191-17202	NN	denotes	compartment
T12142	17203-17205	IN	denotes	of
T12143	17206-17220	NNS	denotes	photoreceptors
T12144	17221-17224	CC	denotes	and
T12145	17225-17228	DT	denotes	the
T12146	17229-17243	NN	denotes	colocalization
T12147	17244-17246	IN	denotes	of
T12148	17247-17253	NN	denotes	RanBP2
T12149	17254-17257	CC	denotes	and
T12150	17258-17263	NN	denotes	Cox11
T12151	17264-17268	IN	denotes	with
T12152	17269-17275	NN	denotes	mHsp70
T12153	17276-17277	-LRB-	denotes	(
T12154	17277-17278	NN	denotes	I
T12155	17279-17282	CC	denotes	and
T12156	17283-17284	NN	denotes	L
T12157	17284-17285	-RRB-	denotes	)
T12158	17285-17287	,	denotes	,
T12159	17287-17292	IN	denotes	while
T12161	17293-17296	NN	denotes	HKI
T12162	17297-17311	NN	denotes	colocalization
T12163	17312-17316	IN	denotes	with
T12164	17317-17322	NN	denotes	Cox11
T12165	17323-17326	VBD	denotes	was
T12160	17327-17334	VBN	denotes	limited
T12166	17335-17337	IN	denotes	to
T12167	17338-17348	VBN	denotes	restricted
T12168	17349-17353	NNS	denotes	foci
T12169	17354-17355	-LRB-	denotes	(
T12171	17355-17356	NN	denotes	R
T12172	17356-17358	,	denotes	,
T12170	17358-17368	NNS	denotes	arrowheads
T12173	17368-17369	-RRB-	denotes	)
T12174	17369-17370	.	denotes	.
T12175	17370-17599	sentence	denotes	High-resolution images of dissociated primary cerebral neurons and glial cells confirmed that the colocalization of HKI and Cox11 was highly restricted (P–R), while RanBP2 extensively colocalized with HKI (S–U) and mHsp70 (V–Z).
T12176	17371-17375	JJ	denotes	High
T12178	17375-17376	HYPH	denotes	-
T12177	17376-17386	NN	denotes	resolution
T12179	17387-17393	NNS	denotes	images
T12181	17394-17396	IN	denotes	of
T12182	17397-17408	VBN	denotes	dissociated
T12184	17409-17416	JJ	denotes	primary
T12185	17417-17425	JJ	denotes	cerebral
T12183	17426-17433	NNS	denotes	neurons
T12186	17434-17437	CC	denotes	and
T12187	17438-17443	JJ	denotes	glial
T12188	17444-17449	NNS	denotes	cells
T12180	17450-17459	VBD	denotes	confirmed
T12189	17460-17464	IN	denotes	that
T12191	17465-17468	DT	denotes	the
T12192	17469-17483	NN	denotes	colocalization
T12193	17484-17486	IN	denotes	of
T12194	17487-17490	NN	denotes	HKI
T12195	17491-17494	CC	denotes	and
T12196	17495-17500	NN	denotes	Cox11
T12190	17501-17504	VBD	denotes	was
T12197	17505-17511	RB	denotes	highly
T12198	17512-17522	JJ	denotes	restricted
T12199	17523-17524	-LRB-	denotes	(
T12200	17524-17525	NN	denotes	P
T12201	17525-17526	SYM	denotes	–
T12202	17526-17527	NN	denotes	R
T12203	17527-17528	-RRB-	denotes	)
T12204	17528-17530	,	denotes	,
T12205	17530-17535	IN	denotes	while
T12207	17536-17542	NN	denotes	RanBP2
T12208	17543-17554	RB	denotes	extensively
T12206	17555-17566	VBD	denotes	colocalized
T12209	17567-17571	IN	denotes	with
T12210	17572-17575	NN	denotes	HKI
T12211	17576-17577	-LRB-	denotes	(
T12212	17577-17578	NN	denotes	S
T12213	17578-17579	SYM	denotes	–
T12214	17579-17580	NN	denotes	U
T12215	17580-17581	-RRB-	denotes	)
T12216	17582-17585	CC	denotes	and
T12217	17586-17592	NN	denotes	mHsp70
T12218	17593-17594	-LRB-	denotes	(
T12219	17594-17595	NN	denotes	V
T12220	17595-17596	SYM	denotes	–
T12221	17596-17597	NN	denotes	Z
T12222	17597-17598	-RRB-	denotes	)
T12223	17598-17599	.	denotes	.
T12224	17599-17657	sentence	denotes	Scale bars in A–O and P–Z are 40 and 10 μm, respectively.
T12225	17600-17605	NN	denotes	Scale
T12226	17606-17610	NNS	denotes	bars
T12228	17611-17613	IN	denotes	in
T12229	17614-17615	NN	denotes	A
T12230	17615-17616	SYM	denotes	–
T12231	17616-17617	NN	denotes	O
T12232	17618-17621	CC	denotes	and
T12233	17622-17623	NN	denotes	P
T12234	17623-17624	SYM	denotes	–
T12235	17624-17625	NN	denotes	Z
T12227	17626-17629	VBP	denotes	are
T12236	17630-17632	CD	denotes	40
T12238	17633-17636	CC	denotes	and
T12239	17637-17639	CD	denotes	10
T12237	17640-17642	NNS	denotes	μm
T12240	17642-17644	,	denotes	,
T12241	17644-17656	RB	denotes	respectively
T12242	17656-17657	.	denotes	.
T12243	17657-17683	sentence	denotes	ONL, outer nuclear layer.
T12244	17658-17661	NN	denotes	ONL
T12245	17661-17663	,	denotes	,
T12246	17663-17668	JJ	denotes	outer
T12248	17669-17676	JJ	denotes	nuclear
T12247	17677-17682	NN	denotes	layer
T12249	17682-17683	.	denotes	.
T3449	17685-17703	NN	denotes	Haploinsufficiency
T3451	17704-17706	IN	denotes	of
T3452	17707-17713	NN	denotes	RanBP2
T3450	17714-17720	VBZ	denotes	Causes
T3453	17721-17730	VBN	denotes	Decreased
T3454	17731-17737	NNS	denotes	Levels
T3455	17738-17740	IN	denotes	of
T3456	17741-17744	NN	denotes	HKI
T3457	17745-17748	CC	denotes	and
T3458	17749-17756	JJ	denotes	Partial
T3459	17757-17772	NN	denotes	Mislocalization
T3460	17773-17775	IN	denotes	of
T3461	17776-17779	NN	denotes	HKI
T3462	17779-18094	sentence	denotes	To determine the physiological implications of the interaction between RanBP2 and HKI (and other partners), we employed a murine embryonic stem 129Ola cell line with a targeted RanBP2 locus produced by gene-trapping to produce stable inbred (coisogenic) and mixed background lines, respectively (Figure 4A and 4B).
T3463	17780-17782	TO	denotes	To
T3464	17783-17792	VB	denotes	determine
T3466	17793-17796	DT	denotes	the
T3468	17797-17810	JJ	denotes	physiological
T3467	17811-17823	NNS	denotes	implications
T3469	17824-17826	IN	denotes	of
T3470	17827-17830	DT	denotes	the
T3471	17831-17842	NN	denotes	interaction
T3472	17843-17850	IN	denotes	between
T3473	17851-17857	NN	denotes	RanBP2
T3474	17858-17861	CC	denotes	and
T3475	17862-17865	NN	denotes	HKI
T3476	17866-17867	-LRB-	denotes	(
T3477	17867-17870	CC	denotes	and
T3478	17871-17876	JJ	denotes	other
T3479	17877-17885	NNS	denotes	partners
T3480	17885-17886	-RRB-	denotes	)
T3481	17886-17888	,	denotes	,
T3482	17888-17890	PRP	denotes	we
T3465	17891-17899	VBD	denotes	employed
T3483	17900-17901	DT	denotes	a
T3485	17902-17908	JJ	denotes	murine
T3486	17909-17918	JJ	denotes	embryonic
T3487	17919-17923	NN	denotes	stem
T3488	17924-17930	NN	denotes	129Ola
T3489	17931-17935	NN	denotes	cell
T3484	17936-17940	NN	denotes	line
T3490	17941-17945	IN	denotes	with
T3491	17946-17947	DT	denotes	a
T3493	17948-17956	VBN	denotes	targeted
T3494	17957-17963	NN	denotes	RanBP2
T3492	17964-17969	NN	denotes	locus
T3495	17970-17978	VBN	denotes	produced
T3496	17979-17981	IN	denotes	by
T3497	17982-17986	NN	denotes	gene
T3499	17986-17987	HYPH	denotes	-
T3498	17987-17995	NN	denotes	trapping
T3500	17996-17998	TO	denotes	to
T3501	17999-18006	VB	denotes	produce
T3502	18007-18013	JJ	denotes	stable
T3504	18014-18020	JJ	denotes	inbred
T3505	18021-18022	-LRB-	denotes	(
T3506	18022-18032	JJ	denotes	coisogenic
T3507	18032-18033	-RRB-	denotes	)
T3508	18034-18037	CC	denotes	and
T3509	18038-18043	JJ	denotes	mixed
T3510	18044-18054	NN	denotes	background
T3503	18055-18060	NNS	denotes	lines
T3511	18060-18062	,	denotes	,
T3512	18062-18074	RB	denotes	respectively
T3513	18075-18076	-LRB-	denotes	(
T3515	18076-18082	NN	denotes	Figure
T3514	18083-18085	NN	denotes	4A
T3516	18086-18089	CC	denotes	and
T3517	18090-18092	NN	denotes	4B
T3518	18092-18093	-RRB-	denotes	)
T3519	18093-18094	.	denotes	.
T3520	18094-18302	sentence	denotes	Genotyping of 299 F2 offspring revealed a RanBP2+/+:RanBP2+/−:RanBP2−/− distribution (89:210:0) that deviated from the expected Mendelian ratio and supports that the RanBP2−/− mice were embryonically lethal.
T3521	18095-18105	NN	denotes	Genotyping
T3523	18106-18108	IN	denotes	of
T3524	18109-18112	CD	denotes	299
T3525	18113-18115	NN	denotes	F2
T3526	18116-18125	NN	denotes	offspring
T3522	18126-18134	VBD	denotes	revealed
T3527	18135-18136	DT	denotes	a
T3529	18137-18143	NN	denotes	RanBP2
T3530	18143-18144	SYM	denotes	+
T3531	18144-18145	HYPH	denotes	/
T3532	18145-18146	SYM	denotes	+
T3533	18146-18147	SYM	denotes	:
T3534	18147-18153	NN	denotes	RanBP2
T3535	18153-18154	SYM	denotes	+
T3536	18154-18155	HYPH	denotes	/
T3537	18155-18156	SYM	denotes	−
T3538	18156-18157	SYM	denotes	:
T3539	18157-18163	NN	denotes	RanBP2
T3540	18163-18164	SYM	denotes	−
T3541	18164-18165	HYPH	denotes	/
T3542	18165-18166	SYM	denotes	−
T3528	18167-18179	NN	denotes	distribution
T3543	18180-18181	-LRB-	denotes	(
T3544	18181-18183	CD	denotes	89
T3546	18183-18184	SYM	denotes	:
T3547	18184-18187	CD	denotes	210
T3548	18187-18188	SYM	denotes	:
T3545	18188-18189	CD	denotes	0
T3549	18189-18190	-RRB-	denotes	)
T3550	18191-18195	WDT	denotes	that
T3551	18196-18204	VBD	denotes	deviated
T3552	18205-18209	IN	denotes	from
T3553	18210-18213	DT	denotes	the
T3555	18214-18222	VBN	denotes	expected
T3556	18223-18232	JJ	denotes	Mendelian
T3554	18233-18238	NN	denotes	ratio
T3557	18239-18242	CC	denotes	and
T3558	18243-18251	VBZ	denotes	supports
T3559	18252-18256	IN	denotes	that
T3561	18257-18260	DT	denotes	the
T3563	18261-18267	NN	denotes	RanBP2
T3564	18267-18268	SYM	denotes	−
T3565	18268-18269	HYPH	denotes	/
T3566	18269-18270	SYM	denotes	−
T3562	18271-18275	NNS	denotes	mice
T3560	18276-18280	VBD	denotes	were
T3567	18281-18294	RB	denotes	embryonically
T3568	18295-18301	JJ	denotes	lethal
T3569	18301-18302	.	denotes	.
T3570	18302-18468	sentence	denotes	E12.5 embryos show strong expression of RanBP2 in the optic vesicle and throughout much of the embryo, which had no apparent developmental abnormalities (Figure 4C).
T3571	18303-18308	NN	denotes	E12.5
T3572	18309-18316	NNS	denotes	embryos
T3573	18317-18321	VBP	denotes	show
T3574	18322-18328	JJ	denotes	strong
T3575	18329-18339	NN	denotes	expression
T3576	18340-18342	IN	denotes	of
T3577	18343-18349	NN	denotes	RanBP2
T3578	18350-18352	IN	denotes	in
T3579	18353-18356	DT	denotes	the
T3581	18357-18362	JJ	denotes	optic
T3580	18363-18370	NN	denotes	vesicle
T3582	18371-18374	CC	denotes	and
T3583	18375-18385	IN	denotes	throughout
T3584	18386-18390	JJ	denotes	much
T3585	18391-18393	IN	denotes	of
T3586	18394-18397	DT	denotes	the
T3587	18398-18404	NN	denotes	embryo
T3588	18404-18406	,	denotes	,
T3589	18406-18411	WDT	denotes	which
T3590	18412-18415	VBD	denotes	had
T3591	18416-18418	DT	denotes	no
T3593	18419-18427	JJ	denotes	apparent
T3594	18428-18441	JJ	denotes	developmental
T3592	18442-18455	NNS	denotes	abnormalities
T3595	18456-18457	-LRB-	denotes	(
T3597	18457-18463	NN	denotes	Figure
T3596	18464-18466	NN	denotes	4C
T3598	18466-18467	-RRB-	denotes	)
T3599	18467-18468	.	denotes	.
T3600	18468-18684	sentence	denotes	In agreement with previous immunocytochemistry analysis [19], the RanBP2 gene is expressed across mature retinal neurons, but expression in ganglion cells of the adult retina was extremely strong (Figure 4D and 4E).
T3601	18469-18471	IN	denotes	In
T3603	18472-18481	NN	denotes	agreement
T3604	18482-18486	IN	denotes	with
T3605	18487-18495	JJ	denotes	previous
T3607	18496-18515	NN	denotes	immunocytochemistry
T3606	18516-18524	NN	denotes	analysis
T3608	18525-18526	-LRB-	denotes	[
T3609	18526-18528	CD	denotes	19
T3610	18528-18529	-RRB-	denotes	]
T3611	18529-18531	,	denotes	,
T3612	18531-18534	DT	denotes	the
T3614	18535-18541	NN	denotes	RanBP2
T3613	18542-18546	NN	denotes	gene
T3615	18547-18549	VBZ	denotes	is
T3602	18550-18559	VBN	denotes	expressed
T3616	18560-18566	IN	denotes	across
T3617	18567-18573	JJ	denotes	mature
T3619	18574-18581	JJ	denotes	retinal
T3618	18582-18589	NNS	denotes	neurons
T3620	18589-18591	,	denotes	,
T3621	18591-18594	CC	denotes	but
T3622	18595-18605	NN	denotes	expression
T3624	18606-18608	IN	denotes	in
T3625	18609-18617	NN	denotes	ganglion
T3626	18618-18623	NNS	denotes	cells
T3627	18624-18626	IN	denotes	of
T3628	18627-18630	DT	denotes	the
T3630	18631-18636	JJ	denotes	adult
T3629	18637-18643	NN	denotes	retina
T3623	18644-18647	VBD	denotes	was
T3631	18648-18657	RB	denotes	extremely
T3632	18658-18664	JJ	denotes	strong
T3633	18665-18666	-LRB-	denotes	(
T3635	18666-18672	NN	denotes	Figure
T3634	18673-18675	NN	denotes	4D
T3636	18676-18679	CC	denotes	and
T3637	18680-18682	NN	denotes	4E
T3638	18682-18683	-RRB-	denotes	)
T3639	18683-18684	.	denotes	.
T3640	18684-21091	sentence	denotes	Figure 4 Insertion Mutagenesis of the Murine RanBP2 Gene (A) Diagram of the genomic region of RanBP2 disrupted by insertion trap mutagenesis with a bicistronic reporter vector between exon 1 and 2. The bicistronic transcript produces two proteins under regulation of RanBP2. Upon splicing of RanBP2, a fusion between exon 1 and β-geo (a fusion between the β-gal and neo genes) is generated, while human placental alkaline phophatase (PLAP) is independently translated using the internal ribosome entry site. Consistent with previous studies, the expression of the former is directed to cell bodies, while expression of the latter is targeted to the axonal processes [67,68]. Transcriptional 5′ RACE analysis detects a fusion between exon 1 and β-geo. (B) Southern analysis of the RanBP2 locus of wild-type and heterozygous genomic DNA of tails of F1 mice digested with PpuMI (left panel) and HindIII (right panel) with probes at the 3′ (left panel) and 5′ (right panel) flanking regions of the insertion breakpoint. Q1 is a cosmid containing the RanBP2 gene up to exon 20 [4]. (C) Lateroventral view of a whole-mount stain of a ~12.5 dpc heterozygous embryo for PLAP and β-gal (inset picture) activities. Although PLAP was broadly expressed (e.g., somites, limbs, and CNS), the PLAP and β-Gal (inset picture) expression was particularly high in the optic vesicle (arrow). X-gal single (D) and combined staining with PLAP (E) of a retinal section of a 3-mo-old RanBP2+/− mouse. Consistent with previous immunocytochemistry studies, β-Gal activity is detected in the neuroretinal bodies and inner segment compartment of photoreceptors with conspicuously strong expression in ganglion cells. PLAP expression is found throughout the plexiform/synaptic layers and outer segment of photoreceptors (E). GC, ganglion cell; PLAP, human placental alkaline phophatase; ROS, rod outer segment; RIS, rod inner segment; ONL, outer nuclear layer; OPL, outer plexiform (synaptic) layer; INL, inner nuclear layer; IPL, inner plexiform (synaptic) layer; GC, ganglion cell layer. In light of the association in vivo of RanBP2 with Cox11 and HKI (Figures 1 and 3), profound in vitro modulation of HKI enzymatic activity by RanBP2 and Cox11 (Figure 2), and the critical role of HKI in catalyzing a rate-limiting step of glycolysis, we probed whether RanBP2+/− mice presented disturbances in HKI, Cox11, and energy homeostasis.
T12535	18695-18704	NN	denotes	Insertion
T12536	18705-18716	NN	denotes	Mutagenesis
T12537	18717-18719	IN	denotes	of
T12538	18720-18723	DT	denotes	the
T12540	18724-18730	JJ	denotes	Murine
T12541	18731-18737	NN	denotes	RanBP2
T12539	18738-18742	NN	denotes	Gene
T12542	18742-18883	sentence	denotes	(A) Diagram of the genomic region of RanBP2 disrupted by insertion trap mutagenesis with a bicistronic reporter vector between exon 1 and 2.
T12543	18743-18744	-LRB-	denotes	(
T12544	18744-18745	LS	denotes	A
T12546	18745-18746	-RRB-	denotes	)
T12545	18747-18754	NN	denotes	Diagram
T12547	18755-18757	IN	denotes	of
T12548	18758-18761	DT	denotes	the
T12550	18762-18769	JJ	denotes	genomic
T12549	18770-18776	NN	denotes	region
T12551	18777-18779	IN	denotes	of
T12552	18780-18786	NN	denotes	RanBP2
T12553	18787-18796	VBN	denotes	disrupted
T12554	18797-18799	IN	denotes	by
T12555	18800-18809	NN	denotes	insertion
T12556	18810-18814	NN	denotes	trap
T12557	18815-18826	NN	denotes	mutagenesis
T12558	18827-18831	IN	denotes	with
T12559	18832-18833	DT	denotes	a
T12561	18834-18845	JJ	denotes	bicistronic
T12562	18846-18854	NN	denotes	reporter
T12560	18855-18861	NN	denotes	vector
T12563	18862-18869	IN	denotes	between
T12564	18870-18874	NN	denotes	exon
T12565	18875-18876	CD	denotes	1
T12566	18877-18880	CC	denotes	and
T12567	18881-18882	CD	denotes	2
T12568	18882-18883	.	denotes	.
T12569	18883-18960	sentence	denotes	The bicistronic transcript produces two proteins under regulation of RanBP2.
T12570	18884-18887	DT	denotes	The
T12572	18888-18899	JJ	denotes	bicistronic
T12571	18900-18910	NN	denotes	transcript
T12573	18911-18919	VBZ	denotes	produces
T12574	18920-18923	CD	denotes	two
T12575	18924-18932	NN	denotes	proteins
T12576	18933-18938	IN	denotes	under
T12577	18939-18949	NN	denotes	regulation
T12578	18950-18952	IN	denotes	of
T12579	18953-18959	NN	denotes	RanBP2
T12580	18959-18960	.	denotes	.
T12581	18960-19193	sentence	denotes	Upon splicing of RanBP2, a fusion between exon 1 and β-geo (a fusion between the β-gal and neo genes) is generated, while human placental alkaline phophatase (PLAP) is independently translated using the internal ribosome entry site.
T12582	18961-18965	IN	denotes	Upon
T12584	18966-18974	NN	denotes	splicing
T12585	18975-18977	IN	denotes	of
T12586	18978-18984	NN	denotes	RanBP2
T12587	18984-18986	,	denotes	,
T12588	18986-18987	DT	denotes	a
T12589	18988-18994	NN	denotes	fusion
T12590	18995-19002	IN	denotes	between
T12591	19003-19007	NN	denotes	exon
T12592	19008-19009	CD	denotes	1
T12593	19010-19013	CC	denotes	and
T12594	19014-19015	NN	denotes	β
T12596	19015-19016	HYPH	denotes	-
T12595	19016-19019	NN	denotes	geo
T12597	19020-19021	-LRB-	denotes	(
T12598	19021-19022	DT	denotes	a
T12599	19023-19029	NN	denotes	fusion
T12600	19030-19037	IN	denotes	between
T12601	19038-19041	DT	denotes	the
T12603	19042-19043	NN	denotes	β
T12605	19043-19044	HYPH	denotes	-
T12604	19044-19047	NN	denotes	gal
T12606	19048-19051	CC	denotes	and
T12607	19052-19055	NN	denotes	neo
T12602	19056-19061	NNS	denotes	genes
T12608	19061-19062	-RRB-	denotes	)
T12609	19063-19065	VBZ	denotes	is
T12583	19066-19075	VBN	denotes	generated
T12610	19075-19077	,	denotes	,
T12611	19077-19082	IN	denotes	while
T12613	19083-19088	JJ	denotes	human
T12615	19089-19098	JJ	denotes	placental
T12616	19099-19107	NN	denotes	alkaline
T12614	19108-19118	NN	denotes	phophatase
T12617	19119-19120	-LRB-	denotes	(
T12618	19120-19124	NN	denotes	PLAP
T12619	19124-19125	-RRB-	denotes	)
T12620	19126-19128	VBZ	denotes	is
T12621	19129-19142	RB	denotes	independently
T12612	19143-19153	VBN	denotes	translated
T12622	19154-19159	VBG	denotes	using
T12623	19160-19163	DT	denotes	the
T12625	19164-19172	JJ	denotes	internal
T12627	19173-19181	NN	denotes	ribosome
T12626	19182-19187	NN	denotes	entry
T12624	19188-19192	NN	denotes	site
T12628	19192-19193	.	denotes	.
T12629	19193-19360	sentence	denotes	Consistent with previous studies, the expression of the former is directed to cell bodies, while expression of the latter is targeted to the axonal processes [67,68].
T12630	19194-19204	JJ	denotes	Consistent
T12632	19205-19209	IN	denotes	with
T12633	19210-19218	JJ	denotes	previous
T12634	19219-19226	NNS	denotes	studies
T12635	19226-19228	,	denotes	,
T12636	19228-19231	DT	denotes	the
T12637	19232-19242	NN	denotes	expression
T12638	19243-19245	IN	denotes	of
T12639	19246-19249	DT	denotes	the
T12640	19250-19256	JJ	denotes	former
T12641	19257-19259	VBZ	denotes	is
T12631	19260-19268	VBN	denotes	directed
T12642	19269-19271	IN	denotes	to
T12643	19272-19276	NN	denotes	cell
T12644	19277-19283	NNS	denotes	bodies
T12645	19283-19285	,	denotes	,
T12646	19285-19290	IN	denotes	while
T12648	19291-19301	NN	denotes	expression
T12649	19302-19304	IN	denotes	of
T12650	19305-19308	DT	denotes	the
T12651	19309-19315	JJ	denotes	latter
T12652	19316-19318	VBZ	denotes	is
T12647	19319-19327	VBN	denotes	targeted
T12653	19328-19330	IN	denotes	to
T12654	19331-19334	DT	denotes	the
T12656	19335-19341	JJ	denotes	axonal
T12655	19342-19351	NNS	denotes	processes
T12657	19352-19353	-LRB-	denotes	[
T12659	19353-19355	CD	denotes	67
T12660	19355-19356	,	denotes	,
T12658	19356-19358	CD	denotes	68
T12661	19358-19359	-RRB-	denotes	]
T12662	19359-19360	.	denotes	.
T12663	19360-19436	sentence	denotes	Transcriptional 5′ RACE analysis detects a fusion between exon 1 and β-geo.
T12664	19361-19376	JJ	denotes	Transcriptional
T12666	19377-19378	CD	denotes	5
T12667	19378-19379	SYM	denotes	′
T12668	19380-19384	NN	denotes	RACE
T12665	19385-19393	NN	denotes	analysis
T12669	19394-19401	VBZ	denotes	detects
T12670	19402-19403	DT	denotes	a
T12671	19404-19410	NN	denotes	fusion
T12672	19411-19418	IN	denotes	between
T12673	19419-19423	NN	denotes	exon
T12674	19424-19425	CD	denotes	1
T12675	19426-19429	CC	denotes	and
T12676	19430-19431	NN	denotes	β
T12678	19431-19432	HYPH	denotes	-
T12677	19432-19435	NN	denotes	geo
T12679	19435-19436	.	denotes	.
T12680	19436-19701	sentence	denotes	(B) Southern analysis of the RanBP2 locus of wild-type and heterozygous genomic DNA of tails of F1 mice digested with PpuMI (left panel) and HindIII (right panel) with probes at the 3′ (left panel) and 5′ (right panel) flanking regions of the insertion breakpoint.
T12681	19437-19438	-LRB-	denotes	(
T12682	19438-19439	LS	denotes	B
T12684	19439-19440	-RRB-	denotes	)
T12685	19441-19449	NNP	denotes	Southern
T12683	19450-19458	NN	denotes	analysis
T12686	19459-19461	IN	denotes	of
T12687	19462-19465	DT	denotes	the
T12689	19466-19472	NN	denotes	RanBP2
T12688	19473-19478	NN	denotes	locus
T12690	19479-19481	IN	denotes	of
T12691	19482-19486	JJ	denotes	wild
T12693	19486-19487	HYPH	denotes	-
T12692	19487-19491	NN	denotes	type
T12695	19492-19495	CC	denotes	and
T12696	19496-19508	JJ	denotes	heterozygous
T12697	19509-19516	JJ	denotes	genomic
T12694	19517-19520	NN	denotes	DNA
T12698	19521-19523	IN	denotes	of
T12699	19524-19529	NNS	denotes	tails
T12700	19530-19532	IN	denotes	of
T12701	19533-19535	NN	denotes	F1
T12702	19536-19540	NNS	denotes	mice
T12703	19541-19549	VBN	denotes	digested
T12704	19550-19554	IN	denotes	with
T12705	19555-19560	NN	denotes	PpuMI
T12706	19561-19562	-LRB-	denotes	(
T12708	19562-19566	JJ	denotes	left
T12707	19567-19572	NN	denotes	panel
T12709	19572-19573	-RRB-	denotes	)
T12710	19574-19577	CC	denotes	and
T12711	19578-19585	NN	denotes	HindIII
T12712	19586-19587	-LRB-	denotes	(
T12714	19587-19592	JJ	denotes	right
T12713	19593-19598	NN	denotes	panel
T12715	19598-19599	-RRB-	denotes	)
T12716	19600-19604	IN	denotes	with
T12717	19605-19611	NNS	denotes	probes
T12718	19612-19614	IN	denotes	at
T12719	19615-19618	DT	denotes	the
T12721	19619-19620	CD	denotes	3
T12722	19620-19621	SYM	denotes	′
T12723	19622-19623	-LRB-	denotes	(
T12725	19623-19627	JJ	denotes	left
T12724	19628-19633	NN	denotes	panel
T12726	19633-19634	-RRB-	denotes	)
T12727	19635-19638	CC	denotes	and
T12728	19639-19640	CD	denotes	5
T12729	19640-19641	SYM	denotes	′
T12730	19642-19643	-LRB-	denotes	(
T12732	19643-19648	JJ	denotes	right
T12731	19649-19654	NN	denotes	panel
T12733	19654-19655	-RRB-	denotes	)
T12734	19656-19664	NN	denotes	flanking
T12720	19665-19672	NNS	denotes	regions
T12735	19673-19675	IN	denotes	of
T12736	19676-19679	DT	denotes	the
T12738	19680-19689	NN	denotes	insertion
T12737	19690-19700	NN	denotes	breakpoint
T12739	19700-19701	.	denotes	.
T12740	19701-19762	sentence	denotes	Q1 is a cosmid containing the RanBP2 gene up to exon 20 [4].
T12741	19702-19704	NN	denotes	Q1
T12742	19705-19707	VBZ	denotes	is
T12743	19708-19709	DT	denotes	a
T12744	19710-19716	NN	denotes	cosmid
T12745	19717-19727	VBG	denotes	containing
T12746	19728-19731	DT	denotes	the
T12748	19732-19738	NN	denotes	RanBP2
T12747	19739-19743	NN	denotes	gene
T12749	19744-19746	IN	denotes	up
T12750	19747-19749	IN	denotes	to
T12751	19750-19754	NN	denotes	exon
T12752	19755-19757	CD	denotes	20
T12753	19758-19759	-LRB-	denotes	[
T12754	19759-19760	CD	denotes	4
T12755	19760-19761	-RRB-	denotes	]
T12756	19761-19762	.	denotes	.
T12757	19762-19890	sentence	denotes	(C) Lateroventral view of a whole-mount stain of a ~12.5 dpc heterozygous embryo for PLAP and β-gal (inset picture) activities.
T12758	19763-19764	-LRB-	denotes	(
T12759	19764-19765	LS	denotes	C
T12761	19765-19766	-RRB-	denotes	)
T12762	19767-19780	JJ	denotes	Lateroventral
T12760	19781-19785	NN	denotes	view
T12763	19786-19788	IN	denotes	of
T12764	19789-19790	DT	denotes	a
T12766	19791-19796	JJ	denotes	whole
T12768	19796-19797	HYPH	denotes	-
T12767	19797-19802	NN	denotes	mount
T12765	19803-19808	NN	denotes	stain
T12769	19809-19811	IN	denotes	of
T12770	19812-19813	DT	denotes	a
T12772	19814-19815	SYM	denotes	~
T12773	19815-19819	CD	denotes	12.5
T12774	19820-19823	NN	denotes	dpc
T12775	19824-19836	JJ	denotes	heterozygous
T12771	19837-19843	NN	denotes	embryo
T12776	19844-19847	IN	denotes	for
T12777	19848-19852	NN	denotes	PLAP
T12779	19853-19856	CC	denotes	and
T12780	19857-19858	NN	denotes	β
T12782	19858-19859	HYPH	denotes	-
T12781	19859-19862	NN	denotes	gal
T12783	19863-19864	-LRB-	denotes	(
T12785	19864-19869	NN	denotes	inset
T12784	19870-19877	NN	denotes	picture
T12786	19877-19878	-RRB-	denotes	)
T12778	19879-19889	NNS	denotes	activities
T12787	19889-19890	.	denotes	.
T12788	19890-20057	sentence	denotes	Although PLAP was broadly expressed (e.g., somites, limbs, and CNS), the PLAP and β-Gal (inset picture) expression was particularly high in the optic vesicle (arrow).
T12789	19891-19899	IN	denotes	Although
T12791	19900-19904	NN	denotes	PLAP
T12792	19905-19908	VBD	denotes	was
T12793	19909-19916	RB	denotes	broadly
T12790	19917-19926	VBN	denotes	expressed
T12795	19927-19928	-LRB-	denotes	(
T12796	19928-19932	FW	denotes	e.g.
T12798	19932-19934	,	denotes	,
T12797	19934-19941	NNS	denotes	somites
T12799	19941-19943	,	denotes	,
T12800	19943-19948	NNS	denotes	limbs
T12801	19948-19950	,	denotes	,
T12802	19950-19953	CC	denotes	and
T12803	19954-19957	NN	denotes	CNS
T12804	19957-19958	-RRB-	denotes	)
T12805	19958-19960	,	denotes	,
T12806	19960-19963	DT	denotes	the
T12808	19964-19968	NN	denotes	PLAP
T12809	19969-19972	CC	denotes	and
T12810	19973-19974	NN	denotes	β
T12812	19974-19975	HYPH	denotes	-
T12811	19975-19978	NN	denotes	Gal
T12813	19979-19980	-LRB-	denotes	(
T12815	19980-19985	NN	denotes	inset
T12814	19986-19993	NN	denotes	picture
T12816	19993-19994	-RRB-	denotes	)
T12807	19995-20005	NN	denotes	expression
T12794	20006-20009	VBD	denotes	was
T12817	20010-20022	RB	denotes	particularly
T12818	20023-20027	JJ	denotes	high
T12819	20028-20030	IN	denotes	in
T12820	20031-20034	DT	denotes	the
T12822	20035-20040	JJ	denotes	optic
T12821	20041-20048	NN	denotes	vesicle
T12823	20049-20050	-LRB-	denotes	(
T12824	20050-20055	NN	denotes	arrow
T12825	20055-20056	-RRB-	denotes	)
T12826	20056-20057	.	denotes	.
T12827	20057-20162	sentence	denotes	X-gal single (D) and combined staining with PLAP (E) of a retinal section of a 3-mo-old RanBP2+/− mouse.
T12828	20058-20059	NN	denotes	X
T12830	20059-20060	HYPH	denotes	-
T12829	20060-20063	NN	denotes	gal
T12832	20064-20070	JJ	denotes	single
T12833	20071-20072	-LRB-	denotes	(
T12834	20072-20073	NN	denotes	D
T12835	20073-20074	-RRB-	denotes	)
T12836	20075-20078	CC	denotes	and
T12837	20079-20087	JJ	denotes	combined
T12831	20088-20096	NN	denotes	staining
T12838	20097-20101	IN	denotes	with
T12839	20102-20106	NN	denotes	PLAP
T12840	20107-20108	-LRB-	denotes	(
T12841	20108-20109	NN	denotes	E
T12842	20109-20110	-RRB-	denotes	)
T12843	20111-20113	IN	denotes	of
T12844	20114-20115	DT	denotes	a
T12846	20116-20123	JJ	denotes	retinal
T12845	20124-20131	NN	denotes	section
T12847	20132-20134	IN	denotes	of
T12848	20135-20136	DT	denotes	a
T12850	20137-20138	CD	denotes	3
T12852	20138-20139	HYPH	denotes	-
T12851	20139-20141	NN	denotes	mo
T12854	20141-20142	HYPH	denotes	-
T12853	20142-20145	JJ	denotes	old
T12855	20146-20152	NN	denotes	RanBP2
T12856	20152-20153	SYM	denotes	+
T12857	20153-20154	HYPH	denotes	/
T12858	20154-20155	SYM	denotes	−
T12849	20156-20161	NN	denotes	mouse
T12859	20161-20162	.	denotes	.
T12860	20162-20374	sentence	denotes	Consistent with previous immunocytochemistry studies, β-Gal activity is detected in the neuroretinal bodies and inner segment compartment of photoreceptors with conspicuously strong expression in ganglion cells.
T12861	20163-20173	JJ	denotes	Consistent
T12863	20174-20178	IN	denotes	with
T12864	20179-20187	JJ	denotes	previous
T12866	20188-20207	NN	denotes	immunocytochemistry
T12865	20208-20215	NNS	denotes	studies
T12867	20215-20217	,	denotes	,
T12868	20217-20218	NN	denotes	β
T12870	20218-20219	HYPH	denotes	-
T12869	20219-20222	NN	denotes	Gal
T12871	20223-20231	NN	denotes	activity
T12872	20232-20234	VBZ	denotes	is
T12862	20235-20243	VBN	denotes	detected
T12873	20244-20246	IN	denotes	in
T12874	20247-20250	DT	denotes	the
T12876	20251-20263	JJ	denotes	neuroretinal
T12875	20264-20270	NNS	denotes	bodies
T12877	20271-20274	CC	denotes	and
T12878	20275-20280	JJ	denotes	inner
T12880	20281-20288	NN	denotes	segment
T12879	20289-20300	NN	denotes	compartment
T12881	20301-20303	IN	denotes	of
T12882	20304-20318	NNS	denotes	photoreceptors
T12883	20319-20323	IN	denotes	with
T12884	20324-20337	RB	denotes	conspicuously
T12885	20338-20344	JJ	denotes	strong
T12886	20345-20355	NN	denotes	expression
T12887	20356-20358	IN	denotes	in
T12888	20359-20367	NN	denotes	ganglion
T12889	20368-20373	NNS	denotes	cells
T12890	20373-20374	.	denotes	.
T12891	20374-20481	sentence	denotes	PLAP expression is found throughout the plexiform/synaptic layers and outer segment of photoreceptors (E).
T12892	20375-20379	NN	denotes	PLAP
T12893	20380-20390	NN	denotes	expression
T12895	20391-20393	VBZ	denotes	is
T12894	20394-20399	VBN	denotes	found
T12896	20400-20410	IN	denotes	throughout
T12897	20411-20414	DT	denotes	the
T12899	20415-20424	JJ	denotes	plexiform
T12901	20424-20425	HYPH	denotes	/
T12900	20425-20433	JJ	denotes	synaptic
T12898	20434-20440	NNS	denotes	layers
T12902	20441-20444	CC	denotes	and
T12903	20445-20450	JJ	denotes	outer
T12904	20451-20458	NN	denotes	segment
T12905	20459-20461	IN	denotes	of
T12906	20462-20476	NNS	denotes	photoreceptors
T12907	20477-20478	-LRB-	denotes	(
T12908	20478-20479	NN	denotes	E
T12909	20479-20480	-RRB-	denotes	)
T12910	20480-20481	.	denotes	.
T12911	20481-20746	sentence	denotes	GC, ganglion cell; PLAP, human placental alkaline phophatase; ROS, rod outer segment; RIS, rod inner segment; ONL, outer nuclear layer; OPL, outer plexiform (synaptic) layer; INL, inner nuclear layer; IPL, inner plexiform (synaptic) layer; GC, ganglion cell layer.
T12912	20482-20484	NN	denotes	GC
T12913	20484-20486	,	denotes	,
T12914	20486-20494	NN	denotes	ganglion
T12915	20495-20499	NN	denotes	cell
T12916	20499-20500	:	denotes	;
T12917	20501-20505	NN	denotes	PLAP
T12918	20505-20507	,	denotes	,
T12919	20507-20512	JJ	denotes	human
T12921	20513-20522	JJ	denotes	placental
T12922	20523-20531	NN	denotes	alkaline
T12920	20532-20542	NN	denotes	phophatase
T12923	20542-20543	:	denotes	;
T12924	20544-20547	NN	denotes	ROS
T12925	20547-20549	,	denotes	,
T12926	20549-20552	NN	denotes	rod
T12928	20553-20558	JJ	denotes	outer
T12927	20559-20566	NN	denotes	segment
T12929	20566-20567	:	denotes	;
T12930	20568-20571	NN	denotes	RIS
T12931	20571-20573	,	denotes	,
T12932	20573-20576	NN	denotes	rod
T12934	20577-20582	JJ	denotes	inner
T12933	20583-20590	NN	denotes	segment
T12935	20590-20591	:	denotes	;
T12936	20592-20595	NN	denotes	ONL
T12937	20595-20597	,	denotes	,
T12938	20597-20602	JJ	denotes	outer
T12940	20603-20610	JJ	denotes	nuclear
T12939	20611-20616	NN	denotes	layer
T12941	20616-20617	:	denotes	;
T12942	20618-20621	NN	denotes	OPL
T12943	20621-20623	,	denotes	,
T12944	20623-20628	JJ	denotes	outer
T12946	20629-20638	JJ	denotes	plexiform
T12947	20639-20640	-LRB-	denotes	(
T12948	20640-20648	JJ	denotes	synaptic
T12949	20648-20649	-RRB-	denotes	)
T12945	20650-20655	NN	denotes	layer
T12950	20655-20656	:	denotes	;
T12951	20657-20660	NN	denotes	INL
T12952	20660-20662	,	denotes	,
T12953	20662-20667	JJ	denotes	inner
T12955	20668-20675	JJ	denotes	nuclear
T12954	20676-20681	NN	denotes	layer
T12956	20681-20682	:	denotes	;
T12957	20683-20686	NN	denotes	IPL
T12958	20686-20688	,	denotes	,
T12959	20688-20693	JJ	denotes	inner
T12961	20694-20703	JJ	denotes	plexiform
T12962	20704-20705	-LRB-	denotes	(
T12963	20705-20713	JJ	denotes	synaptic
T12964	20713-20714	-RRB-	denotes	)
T12960	20715-20720	NN	denotes	layer
T12965	20720-20721	:	denotes	;
T12966	20722-20724	NN	denotes	GC
T12967	20724-20726	,	denotes	,
T12968	20726-20734	NN	denotes	ganglion
T12969	20735-20739	NN	denotes	cell
T12970	20740-20745	NN	denotes	layer
T12971	20745-20746	.	denotes	.
T3641	20747-20749	IN	denotes	In
T3643	20750-20755	NN	denotes	light
T3644	20756-20758	IN	denotes	of
T3645	20759-20762	DT	denotes	the
T3646	20763-20774	NN	denotes	association
T3647	20775-20777	FW	denotes	in
T3648	20778-20782	FW	denotes	vivo
T3649	20783-20785	IN	denotes	of
T3650	20786-20792	NN	denotes	RanBP2
T3651	20793-20797	IN	denotes	with
T3652	20798-20803	NN	denotes	Cox11
T3653	20804-20807	CC	denotes	and
T3654	20808-20811	NN	denotes	HKI
T3655	20812-20813	-LRB-	denotes	(
T3657	20813-20820	NNS	denotes	Figures
T3656	20821-20822	CD	denotes	1
T3658	20823-20826	CC	denotes	and
T3659	20827-20828	CD	denotes	3
T3660	20828-20829	-RRB-	denotes	)
T3661	20829-20831	,	denotes	,
T3662	20831-20839	JJ	denotes	profound
T3664	20840-20842	FW	denotes	in
T3665	20843-20848	FW	denotes	vitro
T3663	20849-20859	NN	denotes	modulation
T3666	20860-20862	IN	denotes	of
T3667	20863-20866	NN	denotes	HKI
T3668	20867-20876	JJ	denotes	enzymatic
T3669	20877-20885	NN	denotes	activity
T3670	20886-20888	IN	denotes	by
T3671	20889-20895	NN	denotes	RanBP2
T3672	20896-20899	CC	denotes	and
T3673	20900-20905	NN	denotes	Cox11
T3674	20906-20907	-LRB-	denotes	(
T3675	20907-20913	NN	denotes	Figure
T3676	20914-20915	CD	denotes	2
T3677	20915-20916	-RRB-	denotes	)
T3678	20916-20918	,	denotes	,
T3679	20918-20921	CC	denotes	and
T3680	20922-20925	DT	denotes	the
T3682	20926-20934	JJ	denotes	critical
T3681	20935-20939	NN	denotes	role
T3683	20940-20942	IN	denotes	of
T3684	20943-20946	NN	denotes	HKI
T3685	20947-20949	IN	denotes	in
T3686	20950-20960	VBG	denotes	catalyzing
T3687	20961-20962	DT	denotes	a
T3689	20963-20967	NN	denotes	rate
T3691	20967-20968	HYPH	denotes	-
T3690	20968-20976	VBG	denotes	limiting
T3688	20977-20981	NN	denotes	step
T3692	20982-20984	IN	denotes	of
T3693	20985-20995	NN	denotes	glycolysis
T3694	20995-20997	,	denotes	,
T3695	20997-20999	PRP	denotes	we
T3642	21000-21006	VBD	denotes	probed
T3696	21007-21014	IN	denotes	whether
T3698	21015-21021	NN	denotes	RanBP2
T3700	21021-21022	SYM	denotes	+
T3701	21022-21023	HYPH	denotes	/
T3702	21023-21024	SYM	denotes	−
T3699	21025-21029	NNS	denotes	mice
T3697	21030-21039	VBD	denotes	presented
T3703	21040-21052	NNS	denotes	disturbances
T3704	21053-21055	IN	denotes	in
T3705	21056-21059	NN	denotes	HKI
T3706	21059-21061	,	denotes	,
T3707	21061-21066	NN	denotes	Cox11
T3708	21066-21068	,	denotes	,
T3709	21068-21071	CC	denotes	and
T3710	21072-21078	NN	denotes	energy
T3711	21079-21090	NN	denotes	homeostasis
T3712	21090-21091	.	denotes	.
T3713	21091-21420	sentence	denotes	Monoallelic expression of RanBP2 does not affect the number of NPCs and their distribution in hippocampal neurons (Figure 5A, unpublished data), but it led to consistent lower expression of RanBP2 by more than 50% in the CNS (brain and retina) in 129Ola (Figure 5B–5D), but not in C57BL/6J/129Ola backgrounds (unpublished data).
T3714	21092-21103	JJ	denotes	Monoallelic
T3715	21104-21114	NN	denotes	expression
T3717	21115-21117	IN	denotes	of
T3718	21118-21124	NN	denotes	RanBP2
T3719	21125-21129	VBZ	denotes	does
T3720	21130-21133	RB	denotes	not
T3716	21134-21140	VB	denotes	affect
T3721	21141-21144	DT	denotes	the
T3722	21145-21151	NN	denotes	number
T3723	21152-21154	IN	denotes	of
T3724	21155-21159	NNS	denotes	NPCs
T3725	21160-21163	CC	denotes	and
T3726	21164-21169	PRP$	denotes	their
T3727	21170-21182	NN	denotes	distribution
T3728	21183-21185	IN	denotes	in
T3729	21186-21197	JJ	denotes	hippocampal
T3730	21198-21205	NNS	denotes	neurons
T3731	21206-21207	-LRB-	denotes	(
T3733	21207-21213	NN	denotes	Figure
T3734	21214-21216	NN	denotes	5A
T3735	21216-21218	,	denotes	,
T3736	21218-21229	JJ	denotes	unpublished
T3732	21230-21234	NNS	denotes	data
T3737	21234-21235	-RRB-	denotes	)
T3738	21235-21237	,	denotes	,
T3739	21237-21240	CC	denotes	but
T3740	21241-21243	PRP	denotes	it
T3741	21244-21247	VBD	denotes	led
T3742	21248-21250	IN	denotes	to
T3743	21251-21261	JJ	denotes	consistent
T3745	21262-21267	JJR	denotes	lower
T3744	21268-21278	NN	denotes	expression
T3746	21279-21281	IN	denotes	of
T3747	21282-21288	NN	denotes	RanBP2
T3748	21289-21291	IN	denotes	by
T3749	21292-21296	JJR	denotes	more
T3751	21297-21301	IN	denotes	than
T3750	21302-21304	CD	denotes	50
T3752	21304-21305	NN	denotes	%
T3753	21306-21308	IN	denotes	in
T3754	21309-21312	DT	denotes	the
T3755	21313-21316	NN	denotes	CNS
T3756	21317-21318	-LRB-	denotes	(
T3757	21318-21323	NN	denotes	brain
T3758	21324-21327	CC	denotes	and
T3759	21328-21334	NN	denotes	retina
T3760	21334-21335	-RRB-	denotes	)
T3761	21336-21338	IN	denotes	in
T3762	21339-21345	NN	denotes	129Ola
T3763	21346-21347	-LRB-	denotes	(
T3765	21347-21353	NN	denotes	Figure
T3764	21354-21356	NN	denotes	5B
T3766	21356-21357	SYM	denotes	–
T3767	21357-21359	NN	denotes	5D
T3768	21359-21360	-RRB-	denotes	)
T3769	21360-21362	,	denotes	,
T3770	21362-21365	CC	denotes	but
T3771	21366-21369	RB	denotes	not
T3772	21370-21372	IN	denotes	in
T3773	21373-21378	NN	denotes	C57BL
T3775	21378-21379	HYPH	denotes	/
T3776	21379-21381	NN	denotes	6J
T3777	21381-21382	HYPH	denotes	/
T3774	21382-21388	NN	denotes	129Ola
T3778	21389-21400	NNS	denotes	backgrounds
T3779	21401-21402	-LRB-	denotes	(
T3781	21402-21413	JJ	denotes	unpublished
T3780	21414-21418	NNS	denotes	data
T3782	21418-21419	-RRB-	denotes	)
T3783	21419-21420	.	denotes	.
T3784	21420-21494	sentence	denotes	Hence, we focused our analysis on the inbred RanBP2+/− 129Ola mouse line.
T3785	21421-21426	RB	denotes	Hence
T3787	21426-21428	,	denotes	,
T3788	21428-21430	PRP	denotes	we
T3786	21431-21438	VBD	denotes	focused
T3789	21439-21442	PRP$	denotes	our
T3790	21443-21451	NN	denotes	analysis
T3791	21452-21454	IN	denotes	on
T3792	21455-21458	DT	denotes	the
T3794	21459-21465	JJ	denotes	inbred
T3795	21466-21472	NN	denotes	RanBP2
T3796	21472-21473	SYM	denotes	+
T3797	21473-21474	HYPH	denotes	/
T3798	21474-21475	SYM	denotes	−
T3799	21476-21482	NN	denotes	129Ola
T3800	21483-21488	NN	denotes	mouse
T3793	21489-21493	NN	denotes	line
T3801	21493-21494	.	denotes	.
T3802	21494-21751	sentence	denotes	Although the levels of RanBP2 were decreased in the retina, brain, and hippocampus of RanBP2+/−mice by ~50%–60% (Figure 5B and 5C), the levels of others nucleoporins, Nup153 and Nup62, and mHsp70 and Cox11, remained unchanged (Figure 5B, unpublished data).
T3803	21495-21503	IN	denotes	Although
T3805	21504-21507	DT	denotes	the
T3806	21508-21514	NNS	denotes	levels
T3807	21515-21517	IN	denotes	of
T3808	21518-21524	NN	denotes	RanBP2
T3809	21525-21529	VBD	denotes	were
T3804	21530-21539	VBN	denotes	decreased
T3811	21540-21542	IN	denotes	in
T3812	21543-21546	DT	denotes	the
T3813	21547-21553	NN	denotes	retina
T3814	21553-21555	,	denotes	,
T3815	21555-21560	NN	denotes	brain
T3816	21560-21562	,	denotes	,
T3817	21562-21565	CC	denotes	and
T3818	21566-21577	NN	denotes	hippocampus
T3819	21578-21580	IN	denotes	of
T3820	21581-21587	NN	denotes	RanBP2
T3822	21587-21588	SYM	denotes	+
T3823	21588-21589	HYPH	denotes	/
T3824	21589-21590	SYM	denotes	−
T3821	21590-21594	NNS	denotes	mice
T3825	21595-21597	IN	denotes	by
T3826	21598-21599	SYM	denotes	~
T3827	21599-21601	CD	denotes	50
T3828	21601-21602	NN	denotes	%
T3829	21602-21603	SYM	denotes	–
T3831	21603-21605	CD	denotes	60
T3830	21605-21606	NN	denotes	%
T3832	21607-21608	-LRB-	denotes	(
T3834	21608-21614	NN	denotes	Figure
T3833	21615-21617	NN	denotes	5B
T3835	21618-21621	CC	denotes	and
T3836	21622-21624	NN	denotes	5C
T3837	21624-21625	-RRB-	denotes	)
T3838	21625-21627	,	denotes	,
T3839	21627-21630	DT	denotes	the
T3840	21631-21637	NNS	denotes	levels
T3841	21638-21640	IN	denotes	of
T3842	21641-21647	NNS	denotes	others
T3843	21648-21660	NNS	denotes	nucleoporins
T3844	21660-21662	,	denotes	,
T3845	21662-21668	NN	denotes	Nup153
T3846	21669-21672	CC	denotes	and
T3847	21673-21678	NN	denotes	Nup62
T3848	21678-21680	,	denotes	,
T3849	21680-21683	CC	denotes	and
T3850	21684-21690	NN	denotes	mHsp70
T3851	21691-21694	CC	denotes	and
T3852	21695-21700	NN	denotes	Cox11
T3853	21700-21702	,	denotes	,
T3810	21702-21710	VBD	denotes	remained
T3854	21711-21720	JJ	denotes	unchanged
T3855	21721-21722	-LRB-	denotes	(
T3857	21722-21728	NN	denotes	Figure
T3858	21729-21731	NN	denotes	5B
T3859	21731-21733	,	denotes	,
T3860	21733-21744	JJ	denotes	unpublished
T3856	21745-21749	NNS	denotes	data
T3861	21749-21750	-RRB-	denotes	)
T3862	21750-21751	.	denotes	.
T3863	21751-21850	sentence	denotes	In addition, we observed a strong decrease of the levels of HKI (3- to 4-fold) (Figure 5B and 5C).
T3864	21752-21754	IN	denotes	In
T3866	21755-21763	NN	denotes	addition
T3867	21763-21765	,	denotes	,
T3868	21765-21767	PRP	denotes	we
T3865	21768-21776	VBD	denotes	observed
T3869	21777-21778	DT	denotes	a
T3871	21779-21785	JJ	denotes	strong
T3870	21786-21794	NN	denotes	decrease
T3872	21795-21797	IN	denotes	of
T3873	21798-21801	DT	denotes	the
T3874	21802-21808	NNS	denotes	levels
T3875	21809-21811	IN	denotes	of
T3876	21812-21815	NN	denotes	HKI
T3877	21816-21817	-LRB-	denotes	(
T3878	21817-21818	CD	denotes	3
T3879	21818-21819	HYPH	denotes	-
T3880	21820-21822	IN	denotes	to
T3881	21823-21829	RB	denotes	4-fold
T3882	21829-21830	-RRB-	denotes	)
T3883	21831-21832	-LRB-	denotes	(
T3885	21832-21838	NN	denotes	Figure
T3884	21839-21841	NN	denotes	5B
T3886	21842-21845	CC	denotes	and
T3887	21846-21848	NN	denotes	5C
T3888	21848-21849	-RRB-	denotes	)
T3889	21849-21850	.	denotes	.
T3890	21850-21990	sentence	denotes	This decrease was selective to the CNS, since HKI levels remained largely unaffected in the skeletal muscle, spleen, and liver (Figure 5D).
T3891	21851-21855	DT	denotes	This
T3892	21856-21864	NN	denotes	decrease
T3893	21865-21868	VBD	denotes	was
T3894	21869-21878	JJ	denotes	selective
T3895	21879-21881	IN	denotes	to
T3896	21882-21885	DT	denotes	the
T3897	21886-21889	NN	denotes	CNS
T3898	21889-21891	,	denotes	,
T3899	21891-21896	IN	denotes	since
T3901	21897-21900	NN	denotes	HKI
T3902	21901-21907	NNS	denotes	levels
T3900	21908-21916	VBD	denotes	remained
T3903	21917-21924	RB	denotes	largely
T3904	21925-21935	JJ	denotes	unaffected
T3905	21936-21938	IN	denotes	in
T3906	21939-21942	DT	denotes	the
T3908	21943-21951	JJ	denotes	skeletal
T3907	21952-21958	NN	denotes	muscle
T3909	21958-21960	,	denotes	,
T3910	21960-21966	NN	denotes	spleen
T3911	21966-21968	,	denotes	,
T3912	21968-21971	CC	denotes	and
T3913	21972-21977	NN	denotes	liver
T3914	21978-21979	-LRB-	denotes	(
T3916	21979-21985	NN	denotes	Figure
T3915	21986-21988	NN	denotes	5D
T3917	21988-21989	-RRB-	denotes	)
T3918	21989-21990	.	denotes	.
T3919	21990-22213	sentence	denotes	Because HKI plays a key role in the production of energy intermediate substrates and HKI is virtually the sole HK isoform expressed in the CNS [33,34], we probed the impact of HKI and RanBP2 reduction in the levels of ATP.
T3920	21991-21998	IN	denotes	Because
T3922	21999-22002	NN	denotes	HKI
T3921	22003-22008	VBZ	denotes	plays
T3924	22009-22010	DT	denotes	a
T3926	22011-22014	JJ	denotes	key
T3925	22015-22019	NN	denotes	role
T3927	22020-22022	IN	denotes	in
T3928	22023-22026	DT	denotes	the
T3929	22027-22037	NN	denotes	production
T3930	22038-22040	IN	denotes	of
T3931	22041-22047	NN	denotes	energy
T3933	22048-22060	JJ	denotes	intermediate
T3932	22061-22071	NNS	denotes	substrates
T3934	22072-22075	CC	denotes	and
T3935	22076-22079	NN	denotes	HKI
T3936	22080-22082	VBZ	denotes	is
T3937	22083-22092	RB	denotes	virtually
T3939	22093-22096	DT	denotes	the
T3940	22097-22101	JJ	denotes	sole
T3941	22102-22104	NN	denotes	HK
T3938	22105-22112	NN	denotes	isoform
T3942	22113-22122	VBN	denotes	expressed
T3943	22123-22125	IN	denotes	in
T3944	22126-22129	DT	denotes	the
T3945	22130-22133	NN	denotes	CNS
T3946	22134-22135	-LRB-	denotes	[
T3948	22135-22137	CD	denotes	33
T3949	22137-22138	,	denotes	,
T3947	22138-22140	CD	denotes	34
T3950	22140-22141	-RRB-	denotes	]
T3951	22141-22143	,	denotes	,
T3952	22143-22145	PRP	denotes	we
T3923	22146-22152	VBD	denotes	probed
T3953	22153-22156	DT	denotes	the
T3954	22157-22163	NN	denotes	impact
T3955	22164-22166	IN	denotes	of
T3956	22167-22170	NN	denotes	HKI
T3958	22171-22174	CC	denotes	and
T3959	22175-22181	NN	denotes	RanBP2
T3957	22182-22191	NN	denotes	reduction
T3960	22192-22194	IN	denotes	in
T3961	22195-22198	DT	denotes	the
T3962	22199-22205	NNS	denotes	levels
T3963	22206-22208	IN	denotes	of
T3964	22209-22212	NN	denotes	ATP
T3965	22212-22213	.	denotes	.
T3966	22213-22364	sentence	denotes	As shown in Figure 5E, there was significant and concordant reduction in levels of ATP in the CNS (brain and retina), but not in non-neuronal tissues.
T3967	22214-22216	IN	denotes	As
T3968	22217-22222	VBN	denotes	shown
T3970	22223-22225	IN	denotes	in
T3971	22226-22232	NN	denotes	Figure
T3972	22233-22235	NN	denotes	5E
T3973	22235-22237	,	denotes	,
T3974	22237-22242	EX	denotes	there
T3969	22243-22246	VBD	denotes	was
T3975	22247-22258	JJ	denotes	significant
T3977	22259-22262	CC	denotes	and
T3978	22263-22273	JJ	denotes	concordant
T3976	22274-22283	NN	denotes	reduction
T3979	22284-22286	IN	denotes	in
T3980	22287-22293	NNS	denotes	levels
T3981	22294-22296	IN	denotes	of
T3982	22297-22300	NN	denotes	ATP
T3983	22301-22303	IN	denotes	in
T3984	22304-22307	DT	denotes	the
T3985	22308-22311	NN	denotes	CNS
T3986	22312-22313	-LRB-	denotes	(
T3987	22313-22318	NN	denotes	brain
T3988	22319-22322	CC	denotes	and
T3989	22323-22329	NN	denotes	retina
T3990	22329-22330	-RRB-	denotes	)
T3991	22330-22332	,	denotes	,
T3992	22332-22335	CC	denotes	but
T3993	22336-22339	RB	denotes	not
T3994	22340-22342	IN	denotes	in
T3995	22343-22355	JJ	denotes	non-neuronal
T3996	22356-22363	NNS	denotes	tissues
T3997	22363-22364	.	denotes	.
T3998	22364-22584	sentence	denotes	Finally, we also observed partial and selective delocalization of HKI, but not of Cox11, from the ellipsoid (mitochondria-rich) to the adjacent myoid subcellular compartment of rod photosensory neurons (Figure S2A–S2E).
T3999	22365-22372	RB	denotes	Finally
T4001	22372-22374	,	denotes	,
T4002	22374-22376	PRP	denotes	we
T4003	22377-22381	RB	denotes	also
T4000	22382-22390	VBD	denotes	observed
T4004	22391-22398	JJ	denotes	partial
T4006	22399-22402	CC	denotes	and
T4007	22403-22412	JJ	denotes	selective
T4005	22413-22427	NN	denotes	delocalization
T4008	22428-22430	IN	denotes	of
T4009	22431-22434	NN	denotes	HKI
T4010	22434-22436	,	denotes	,
T4011	22436-22439	CC	denotes	but
T4012	22440-22443	RB	denotes	not
T4013	22444-22446	IN	denotes	of
T4014	22447-22452	NN	denotes	Cox11
T4015	22452-22454	,	denotes	,
T4016	22454-22458	IN	denotes	from
T4017	22459-22462	DT	denotes	the
T4018	22463-22472	JJ	denotes	ellipsoid
T4019	22473-22474	-LRB-	denotes	(
T4020	22474-22486	NNS	denotes	mitochondria
T4022	22486-22487	HYPH	denotes	-
T4021	22487-22491	JJ	denotes	rich
T4023	22491-22492	-RRB-	denotes	)
T4024	22493-22495	IN	denotes	to
T4025	22496-22499	DT	denotes	the
T4027	22500-22508	JJ	denotes	adjacent
T4026	22509-22514	JJ	denotes	myoid
T4028	22515-22526	JJ	denotes	subcellular
T4029	22527-22538	NN	denotes	compartment
T4030	22539-22541	IN	denotes	of
T4031	22542-22545	NN	denotes	rod
T4033	22546-22558	JJ	denotes	photosensory
T4032	22559-22566	NNS	denotes	neurons
T4034	22567-22568	-LRB-	denotes	(
T4036	22568-22574	NN	denotes	Figure
T4035	22575-22578	NN	denotes	S2A
T4037	22578-22579	SYM	denotes	–
T4038	22579-22582	NN	denotes	S2E
T4039	22582-22583	-RRB-	denotes	)
T4040	22583-22584	.	denotes	.
T4041	22584-22725	sentence	denotes	This was also accompanied by reduced HKI levels in the inner retina, in particular in the inner plexiform (synaptic) layer (Figure S2A–S2E).
T4042	22585-22589	DT	denotes	This
T4044	22590-22593	VBD	denotes	was
T4045	22594-22598	RB	denotes	also
T4043	22599-22610	VBN	denotes	accompanied
T4046	22611-22613	IN	denotes	by
T4047	22614-22621	VBN	denotes	reduced
T4049	22622-22625	NN	denotes	HKI
T4048	22626-22632	NNS	denotes	levels
T4050	22633-22635	IN	denotes	in
T4051	22636-22639	DT	denotes	the
T4053	22640-22645	JJ	denotes	inner
T4052	22646-22652	NN	denotes	retina
T4054	22652-22654	,	denotes	,
T4055	22654-22656	IN	denotes	in
T4057	22657-22667	JJ	denotes	particular
T4056	22668-22670	IN	denotes	in
T4058	22671-22674	DT	denotes	the
T4060	22675-22680	JJ	denotes	inner
T4061	22681-22690	JJ	denotes	plexiform
T4062	22691-22692	-LRB-	denotes	(
T4063	22692-22700	JJ	denotes	synaptic
T4064	22700-22701	-RRB-	denotes	)
T4059	22702-22707	NN	denotes	layer
T4065	22708-22709	-LRB-	denotes	(
T4067	22709-22715	NN	denotes	Figure
T4066	22716-22719	NN	denotes	S2A
T4068	22719-22720	SYM	denotes	–
T4069	22720-22723	NN	denotes	S2E
T4070	22723-22724	-RRB-	denotes	)
T4071	22724-22725	.	denotes	.
T13139	22736-22754	NN	denotes	Haploinsufficiency
T13141	22755-22757	IN	denotes	of
T13142	22758-22764	NN	denotes	RanBP2
T13140	22765-22771	VBZ	denotes	Causes
T13143	22772-22773	DT	denotes	a
T13144	22774-22782	NN	denotes	Decrease
T13145	22783-22785	IN	denotes	in
T13146	22786-22789	NN	denotes	HKI
T13147	22790-22797	NN	denotes	Protein
T13148	22798-22801	CC	denotes	and
T13149	22802-22805	NN	denotes	ATP
T13150	22806-22812	NNS	denotes	Levels
T13151	22812-22961	sentence	denotes	(A) Quantitative analysis of NPCs in dissociated hippocampal neurons of wild-type (+/+) and heterozygote (+/−) mice upon immunostaining with mAb414.
T13152	22813-22814	-LRB-	denotes	(
T13153	22814-22815	LS	denotes	A
T13155	22815-22816	-RRB-	denotes	)
T13156	22817-22829	JJ	denotes	Quantitative
T13154	22830-22838	NN	denotes	analysis
T13157	22839-22841	IN	denotes	of
T13158	22842-22846	NNS	denotes	NPCs
T13159	22847-22849	IN	denotes	in
T13160	22850-22861	VBN	denotes	dissociated
T13162	22862-22873	JJ	denotes	hippocampal
T13161	22874-22881	NNS	denotes	neurons
T13163	22882-22884	IN	denotes	of
T13164	22885-22889	JJ	denotes	wild
T13166	22889-22890	HYPH	denotes	-
T13165	22890-22894	NN	denotes	type
T13168	22895-22896	-LRB-	denotes	(
T13170	22896-22897	SYM	denotes	+
T13171	22897-22898	HYPH	denotes	/
T13169	22898-22899	SYM	denotes	+
T13172	22899-22900	-RRB-	denotes	)
T13173	22901-22904	CC	denotes	and
T13174	22905-22917	NN	denotes	heterozygote
T13175	22918-22919	-LRB-	denotes	(
T13177	22919-22920	SYM	denotes	+
T13178	22920-22921	HYPH	denotes	/
T13176	22921-22922	SYM	denotes	−
T13179	22922-22923	-RRB-	denotes	)
T13167	22924-22928	NNS	denotes	mice
T13180	22929-22933	IN	denotes	upon
T13181	22934-22948	NN	denotes	immunostaining
T13182	22949-22953	IN	denotes	with
T13183	22954-22960	NN	denotes	mAb414
T13184	22960-22961	.	denotes	.
T13185	22961-23084	sentence	denotes	No difference in the density of NPCs (3–4 NPC/μm2) at the nuclear envelope was found between RanBP2+/+ and RanBP2+/− mice.
T13186	22962-22964	DT	denotes	No
T13187	22965-22975	NN	denotes	difference
T13189	22976-22978	IN	denotes	in
T13190	22979-22982	DT	denotes	the
T13191	22983-22990	NN	denotes	density
T13192	22991-22993	IN	denotes	of
T13193	22994-22998	NNS	denotes	NPCs
T13194	22999-23000	-LRB-	denotes	(
T13195	23000-23001	CD	denotes	3
T13197	23001-23002	SYM	denotes	–
T13196	23002-23003	CD	denotes	4
T13198	23004-23007	NN	denotes	NPC
T13199	23007-23008	SYM	denotes	/
T13200	23008-23011	NN	denotes	μm2
T13201	23011-23012	-RRB-	denotes	)
T13202	23013-23015	IN	denotes	at
T13203	23016-23019	DT	denotes	the
T13205	23020-23027	JJ	denotes	nuclear
T13204	23028-23036	NN	denotes	envelope
T13206	23037-23040	VBD	denotes	was
T13188	23041-23046	VBN	denotes	found
T13207	23047-23054	IN	denotes	between
T13208	23055-23061	NN	denotes	RanBP2
T13210	23061-23062	SYM	denotes	+
T13211	23062-23063	HYPH	denotes	/
T13212	23063-23064	SYM	denotes	+
T13213	23065-23068	CC	denotes	and
T13214	23069-23075	NN	denotes	RanBP2
T13215	23075-23076	SYM	denotes	+
T13216	23076-23077	HYPH	denotes	/
T13217	23077-23078	SYM	denotes	−
T13209	23079-23083	NNS	denotes	mice
T13218	23083-23084	.	denotes	.
T13219	23084-23249	sentence	denotes	(B) Immunoblots with anti-RanBP2/Nup153/Nup62 (mAb414), −HKI, −mHsp70, and −Cox11 antibodies of retinal (top panel) and hippocampal homogenates of +/+ and +/− mice.
T13220	23085-23086	-LRB-	denotes	(
T13221	23086-23087	LS	denotes	B
T13223	23087-23088	-RRB-	denotes	)
T13222	23089-23100	NNS	denotes	Immunoblots
T13224	23101-23105	IN	denotes	with
T13225	23106-23117	NN	denotes	anti-RanBP2
T13227	23117-23118	HYPH	denotes	/
T13228	23118-23124	NN	denotes	Nup153
T13229	23124-23125	HYPH	denotes	/
T13226	23125-23130	NN	denotes	Nup62
T13231	23131-23132	-LRB-	denotes	(
T13232	23132-23138	NN	denotes	mAb414
T13233	23138-23139	-RRB-	denotes	)
T13234	23139-23141	,	denotes	,
T13235	23141-23142	SYM	denotes	−
T13236	23142-23145	NN	denotes	HKI
T13237	23145-23147	,	denotes	,
T13238	23147-23148	SYM	denotes	−
T13239	23148-23154	NN	denotes	mHsp70
T13240	23154-23156	,	denotes	,
T13241	23156-23159	CC	denotes	and
T13242	23160-23161	SYM	denotes	−
T13243	23161-23166	NN	denotes	Cox11
T13230	23167-23177	NNS	denotes	antibodies
T13244	23178-23180	IN	denotes	of
T13245	23181-23188	JJ	denotes	retinal
T13247	23189-23190	-LRB-	denotes	(
T13249	23190-23193	JJ	denotes	top
T13248	23194-23199	NN	denotes	panel
T13250	23199-23200	-RRB-	denotes	)
T13251	23201-23204	CC	denotes	and
T13252	23205-23216	JJ	denotes	hippocampal
T13246	23217-23228	NNS	denotes	homogenates
T13253	23229-23231	IN	denotes	of
T13254	23232-23233	SYM	denotes	+
T13256	23233-23234	HYPH	denotes	/
T13255	23234-23235	SYM	denotes	+
T13258	23236-23239	CC	denotes	and
T13259	23240-23241	SYM	denotes	+
T13261	23241-23242	HYPH	denotes	/
T13260	23242-23243	SYM	denotes	−
T13257	23244-23248	NNS	denotes	mice
T13262	23248-23249	.	denotes	.
T13263	23249-23382	sentence	denotes	In comparison to RanBP2+/+, RanBP2+/− mice exhibit a reduction in the expression levels of RanBP2 and HKI but not of other proteins.
T13264	23250-23252	IN	denotes	In
T13266	23253-23263	NN	denotes	comparison
T13267	23264-23266	IN	denotes	to
T13268	23267-23273	NN	denotes	RanBP2
T13269	23273-23274	SYM	denotes	+
T13270	23274-23275	HYPH	denotes	/
T13271	23275-23276	SYM	denotes	+
T13272	23276-23278	,	denotes	,
T13273	23278-23284	NN	denotes	RanBP2
T13275	23284-23285	SYM	denotes	+
T13276	23285-23286	HYPH	denotes	/
T13277	23286-23287	SYM	denotes	−
T13274	23288-23292	NNS	denotes	mice
T13265	23293-23300	VBP	denotes	exhibit
T13278	23301-23302	DT	denotes	a
T13279	23303-23312	NN	denotes	reduction
T13280	23313-23315	IN	denotes	in
T13281	23316-23319	DT	denotes	the
T13283	23320-23330	NN	denotes	expression
T13282	23331-23337	NNS	denotes	levels
T13284	23338-23340	IN	denotes	of
T13285	23341-23347	NN	denotes	RanBP2
T13286	23348-23351	CC	denotes	and
T13287	23352-23355	NN	denotes	HKI
T13288	23356-23359	CC	denotes	but
T13289	23360-23363	RB	denotes	not
T13290	23364-23366	IN	denotes	of
T13291	23367-23372	JJ	denotes	other
T13292	23373-23381	NN	denotes	proteins
T13293	23381-23382	.	denotes	.
T13294	23382-23531	sentence	denotes	(C) Quantitative analysis of relative protein expression levels of RanBP2, Cox11, HKI, and mHsp70 in the hippocampus of RanBP2+/+ and RanBP2+/−mice.
T13295	23383-23384	-LRB-	denotes	(
T13296	23384-23385	LS	denotes	C
T13298	23385-23386	-RRB-	denotes	)
T13299	23387-23399	JJ	denotes	Quantitative
T13297	23400-23408	NN	denotes	analysis
T13300	23409-23411	IN	denotes	of
T13301	23412-23420	JJ	denotes	relative
T13303	23421-23428	NN	denotes	protein
T13304	23429-23439	NN	denotes	expression
T13302	23440-23446	NNS	denotes	levels
T13305	23447-23449	IN	denotes	of
T13306	23450-23456	NN	denotes	RanBP2
T13307	23456-23458	,	denotes	,
T13308	23458-23463	NN	denotes	Cox11
T13309	23463-23465	,	denotes	,
T13310	23465-23468	NN	denotes	HKI
T13311	23468-23470	,	denotes	,
T13312	23470-23473	CC	denotes	and
T13313	23474-23480	NN	denotes	mHsp70
T13314	23481-23483	IN	denotes	in
T13315	23484-23487	DT	denotes	the
T13316	23488-23499	NN	denotes	hippocampus
T13317	23500-23502	IN	denotes	of
T13318	23503-23509	NN	denotes	RanBP2
T13320	23509-23510	SYM	denotes	+
T13321	23510-23511	HYPH	denotes	/
T13322	23511-23512	SYM	denotes	+
T13323	23513-23516	CC	denotes	and
T13324	23517-23523	NN	denotes	RanBP2
T13325	23523-23524	SYM	denotes	+
T13326	23524-23525	HYPH	denotes	/
T13327	23525-23526	SYM	denotes	−
T13319	23526-23530	NNS	denotes	mice
T13328	23530-23531	.	denotes	.
T13329	23531-23605	sentence	denotes	There is ~2- and 4-fold reduction of RanBP2 and HKI in heterozygote mice.
T13330	23532-23537	EX	denotes	There
T13331	23538-23540	VBZ	denotes	is
T13332	23541-23542	SYM	denotes	~
T13333	23542-23543	CD	denotes	2
T13335	23543-23544	HYPH	denotes	-
T13336	23545-23548	CC	denotes	and
T13337	23549-23555	RB	denotes	4-fold
T13334	23556-23565	NN	denotes	reduction
T13338	23566-23568	IN	denotes	of
T13339	23569-23575	NN	denotes	RanBP2
T13340	23576-23579	CC	denotes	and
T13341	23580-23583	NN	denotes	HKI
T13342	23584-23586	IN	denotes	in
T13343	23587-23599	NN	denotes	heterozygote
T13344	23600-23604	NNS	denotes	mice
T13345	23604-23605	.	denotes	.
T13346	23605-23724	sentence	denotes	(D) The level of HKI is reduced in the brain but not in other non-neuronal tissues tested (muscle, spleen, and liver).
T13347	23606-23607	-LRB-	denotes	(
T13348	23607-23608	LS	denotes	D
T13350	23608-23609	-RRB-	denotes	)
T13351	23610-23613	DT	denotes	The
T13352	23614-23619	NN	denotes	level
T13353	23620-23622	IN	denotes	of
T13354	23623-23626	NN	denotes	HKI
T13355	23627-23629	VBZ	denotes	is
T13349	23630-23637	VBN	denotes	reduced
T13356	23638-23640	IN	denotes	in
T13357	23641-23644	DT	denotes	the
T13358	23645-23650	NN	denotes	brain
T13359	23651-23654	CC	denotes	but
T13360	23655-23658	RB	denotes	not
T13361	23659-23661	IN	denotes	in
T13362	23662-23667	JJ	denotes	other
T13364	23668-23680	JJ	denotes	non-neuronal
T13363	23681-23688	NNS	denotes	tissues
T13365	23689-23695	VBN	denotes	tested
T13366	23696-23697	-LRB-	denotes	(
T13367	23697-23703	NN	denotes	muscle
T13368	23703-23705	,	denotes	,
T13369	23705-23711	NN	denotes	spleen
T13370	23711-23713	,	denotes	,
T13371	23713-23716	CC	denotes	and
T13372	23717-23722	NN	denotes	liver
T13373	23722-23723	-RRB-	denotes	)
T13374	23723-23724	.	denotes	.
T13375	23724-23852	sentence	denotes	(E) The total ATP level is reduced in the CNS tissues (brain and retina) but not in non-neuronal tissues tested (e.g., spleen).
T13376	23725-23726	-LRB-	denotes	(
T13377	23726-23727	LS	denotes	E
T13379	23727-23728	-RRB-	denotes	)
T13380	23729-23732	DT	denotes	The
T13382	23733-23738	JJ	denotes	total
T13383	23739-23742	NN	denotes	ATP
T13381	23743-23748	NN	denotes	level
T13384	23749-23751	VBZ	denotes	is
T13378	23752-23759	VBN	denotes	reduced
T13385	23760-23762	IN	denotes	in
T13386	23763-23766	DT	denotes	the
T13388	23767-23770	NN	denotes	CNS
T13387	23771-23778	NNS	denotes	tissues
T13389	23779-23780	-LRB-	denotes	(
T13390	23780-23785	NN	denotes	brain
T13391	23786-23789	CC	denotes	and
T13392	23790-23796	NN	denotes	retina
T13393	23796-23797	-RRB-	denotes	)
T13394	23798-23801	CC	denotes	but
T13395	23802-23805	RB	denotes	not
T13396	23806-23808	IN	denotes	in
T13397	23809-23821	JJ	denotes	non-neuronal
T13398	23822-23829	NNS	denotes	tissues
T13399	23830-23836	VBN	denotes	tested
T13400	23837-23838	-LRB-	denotes	(
T13401	23838-23842	FW	denotes	e.g.
T13403	23842-23844	,	denotes	,
T13402	23844-23850	NN	denotes	spleen
T13404	23850-23851	-RRB-	denotes	)
T13405	23851-23852	.	denotes	.
T4308	23854-23863	JJ	denotes	Metabolic
T4309	23864-23876	NNS	denotes	Disturbances
T4310	23877-23883	VBN	denotes	Caused
T4311	23884-23886	IN	denotes	by
T4312	23887-23905	NN	denotes	Haploinsufficiency
T4313	23906-23908	IN	denotes	of
T4314	23909-23915	NN	denotes	RanBP2
T4315	23915-24044	sentence	denotes	The growth rates of inbred RanBP2+/− mice on high-fat (~10% fat) diet were significantly slower than RanBP2+/+ mice (Figure 6A).
T4316	23916-23919	DT	denotes	The
T4318	23920-23926	NN	denotes	growth
T4317	23927-23932	NNS	denotes	rates
T4320	23933-23935	IN	denotes	of
T4321	23936-23942	JJ	denotes	inbred
T4323	23943-23949	NN	denotes	RanBP2
T4324	23949-23950	SYM	denotes	+
T4325	23950-23951	HYPH	denotes	/
T4326	23951-23952	SYM	denotes	−
T4322	23953-23957	NNS	denotes	mice
T4327	23958-23960	IN	denotes	on
T4328	23961-23965	JJ	denotes	high
T4330	23965-23966	HYPH	denotes	-
T4329	23966-23969	NN	denotes	fat
T4332	23970-23971	-LRB-	denotes	(
T4333	23971-23972	SYM	denotes	~
T4334	23972-23974	CD	denotes	10
T4335	23974-23975	NN	denotes	%
T4336	23976-23979	NN	denotes	fat
T4337	23979-23980	-RRB-	denotes	)
T4331	23981-23985	NN	denotes	diet
T4319	23986-23990	VBD	denotes	were
T4338	23991-24004	RB	denotes	significantly
T4339	24005-24011	JJR	denotes	slower
T4340	24012-24016	IN	denotes	than
T4341	24017-24023	NN	denotes	RanBP2
T4343	24023-24024	SYM	denotes	+
T4344	24024-24025	HYPH	denotes	/
T4345	24025-24026	SYM	denotes	+
T4342	24027-24031	NNS	denotes	mice
T4346	24032-24033	-LRB-	denotes	(
T4348	24033-24039	NN	denotes	Figure
T4347	24040-24042	NN	denotes	6A
T4349	24042-24043	-RRB-	denotes	)
T4350	24043-24044	.	denotes	.
T4351	24044-24172	sentence	denotes	Beginning at around 4 mo of age, RanBP2+/− mice exhibit a significant slower gain in body mass than wild-type mice (Figure 6A).
T4352	24045-24054	VBG	denotes	Beginning
T4354	24055-24057	IN	denotes	at
T4355	24058-24064	IN	denotes	around
T4356	24065-24066	CD	denotes	4
T4357	24067-24069	NNS	denotes	mo
T4358	24070-24072	IN	denotes	of
T4359	24073-24076	NN	denotes	age
T4360	24076-24078	,	denotes	,
T4361	24078-24084	NN	denotes	RanBP2
T4363	24084-24085	SYM	denotes	+
T4364	24085-24086	HYPH	denotes	/
T4365	24086-24087	SYM	denotes	−
T4362	24088-24092	NNS	denotes	mice
T4353	24093-24100	VBP	denotes	exhibit
T4366	24101-24102	DT	denotes	a
T4368	24103-24114	JJ	denotes	significant
T4369	24115-24121	JJR	denotes	slower
T4367	24122-24126	NN	denotes	gain
T4370	24127-24129	IN	denotes	in
T4371	24130-24134	NN	denotes	body
T4372	24135-24139	NN	denotes	mass
T4373	24140-24144	IN	denotes	than
T4374	24145-24149	JJ	denotes	wild
T4376	24149-24150	HYPH	denotes	-
T4375	24150-24154	NN	denotes	type
T4377	24155-24159	NNS	denotes	mice
T4378	24160-24161	-LRB-	denotes	(
T4380	24161-24167	NN	denotes	Figure
T4379	24168-24170	NN	denotes	6A
T4381	24170-24171	-RRB-	denotes	)
T4382	24171-24172	.	denotes	.
T4383	24172-24331	sentence	denotes	In addition, RanBP2+/− inbred mice presented deficits in body mass that were erased by changing the genetic background to a mixed C57BL/6J/129Ola (Figure 6B).
T4384	24173-24175	IN	denotes	In
T4386	24176-24184	NN	denotes	addition
T4387	24184-24186	,	denotes	,
T4388	24186-24192	NN	denotes	RanBP2
T4390	24192-24193	SYM	denotes	+
T4391	24193-24194	HYPH	denotes	/
T4392	24194-24195	SYM	denotes	−
T4393	24196-24202	JJ	denotes	inbred
T4389	24203-24207	NNS	denotes	mice
T4385	24208-24217	VBD	denotes	presented
T4394	24218-24226	NNS	denotes	deficits
T4395	24227-24229	IN	denotes	in
T4396	24230-24234	NN	denotes	body
T4397	24235-24239	NN	denotes	mass
T4398	24240-24244	WDT	denotes	that
T4400	24245-24249	VBD	denotes	were
T4399	24250-24256	VBN	denotes	erased
T4401	24257-24259	IN	denotes	by
T4402	24260-24268	VBG	denotes	changing
T4403	24269-24272	DT	denotes	the
T4405	24273-24280	JJ	denotes	genetic
T4404	24281-24291	NN	denotes	background
T4406	24292-24294	IN	denotes	to
T4407	24295-24296	DT	denotes	a
T4409	24297-24302	JJ	denotes	mixed
T4410	24303-24308	NN	denotes	C57BL
T4411	24308-24309	HYPH	denotes	/
T4412	24309-24311	NN	denotes	6J
T4413	24311-24312	HYPH	denotes	/
T4408	24312-24318	NN	denotes	129Ola
T4414	24319-24320	-LRB-	denotes	(
T4416	24320-24326	NN	denotes	Figure
T4415	24327-24329	NN	denotes	6B
T4417	24329-24330	-RRB-	denotes	)
T4418	24330-24331	.	denotes	.
T4419	24331-24418	sentence	denotes	Food consumption did not account for the body weight differences observed (Figure 6C).
T4420	24332-24336	NN	denotes	Food
T4421	24337-24348	NN	denotes	consumption
T4423	24349-24352	VBD	denotes	did
T4424	24353-24356	RB	denotes	not
T4422	24357-24364	VB	denotes	account
T4425	24365-24368	IN	denotes	for
T4426	24369-24372	DT	denotes	the
T4428	24373-24377	NN	denotes	body
T4429	24378-24384	NN	denotes	weight
T4427	24385-24396	NNS	denotes	differences
T4430	24397-24405	VBN	denotes	observed
T4431	24406-24407	-LRB-	denotes	(
T4433	24407-24413	NN	denotes	Figure
T4432	24414-24416	NN	denotes	6C
T4434	24416-24417	-RRB-	denotes	)
T4435	24417-24418	.	denotes	.
T4436	24418-25274	sentence	denotes	Figure 6 RanBP2+/− Mice on High-Fat Diet Exhibit Deficits in Growth (A) In comparison to wild-type mice, RanBP2+/− mice show slower growth rates beginning at 4 mo of age (arrow), and the difference in body weight between these is maintained afterward. Note that RanBP2+/− mice lack the growth spur observed in wild-type mice between 3 and 4 mo of age. (B) In comparison to inbred RanBP2+/−mice (129Ola genetic background), the difference in body weight between RanBP2+/+ and RanBP2+/− mice is masked upon placing these on a mixed 129Ola/C57Bl6 genetic background. (C) RanBP2+/+ and RanBP2+/−inbred mice exhibit similar rates of food consumption. Mice in (A), (B), and (C) were placed on a high-fat diet since birth (n = 5). HKI in the CNS (brain and retina) accounts virtually for all expression of HK isozymes and glucose utilization in the CNS [33,34].
T13498	24429-24435	NN	denotes	RanBP2
T13500	24435-24436	SYM	denotes	+
T13501	24436-24437	HYPH	denotes	/
T13502	24437-24438	SYM	denotes	−
T13499	24439-24443	NNS	denotes	Mice
T13504	24444-24446	IN	denotes	on
T13505	24447-24451	JJ	denotes	High
T13507	24451-24452	HYPH	denotes	-
T13506	24452-24455	NN	denotes	Fat
T13508	24456-24460	NN	denotes	Diet
T13503	24461-24468	VBP	denotes	Exhibit
T13509	24469-24477	NNS	denotes	Deficits
T13510	24478-24480	IN	denotes	in
T13511	24481-24487	NN	denotes	Growth
T13512	24487-24671	sentence	denotes	(A) In comparison to wild-type mice, RanBP2+/− mice show slower growth rates beginning at 4 mo of age (arrow), and the difference in body weight between these is maintained afterward.
T13513	24488-24489	-LRB-	denotes	(
T13514	24489-24490	LS	denotes	A
T13516	24490-24491	-RRB-	denotes	)
T13517	24492-24494	IN	denotes	In
T13518	24495-24505	NN	denotes	comparison
T13519	24506-24508	IN	denotes	to
T13520	24509-24513	JJ	denotes	wild
T13522	24513-24514	HYPH	denotes	-
T13521	24514-24518	NN	denotes	type
T13523	24519-24523	NNS	denotes	mice
T13524	24523-24525	,	denotes	,
T13525	24525-24531	NN	denotes	RanBP2
T13527	24531-24532	SYM	denotes	+
T13528	24532-24533	HYPH	denotes	/
T13529	24533-24534	SYM	denotes	−
T13526	24535-24539	NNS	denotes	mice
T13515	24540-24544	VBP	denotes	show
T13530	24545-24551	JJR	denotes	slower
T13532	24552-24558	NN	denotes	growth
T13531	24559-24564	NNS	denotes	rates
T13533	24565-24574	VBG	denotes	beginning
T13534	24575-24577	IN	denotes	at
T13535	24578-24579	CD	denotes	4
T13536	24580-24582	NNS	denotes	mo
T13537	24583-24585	IN	denotes	of
T13538	24586-24589	NN	denotes	age
T13539	24590-24591	-LRB-	denotes	(
T13540	24591-24596	NN	denotes	arrow
T13541	24596-24597	-RRB-	denotes	)
T13542	24597-24599	,	denotes	,
T13543	24599-24602	CC	denotes	and
T13544	24603-24606	DT	denotes	the
T13545	24607-24617	NN	denotes	difference
T13547	24618-24620	IN	denotes	in
T13548	24621-24625	NN	denotes	body
T13549	24626-24632	NN	denotes	weight
T13550	24633-24640	IN	denotes	between
T13551	24641-24646	DT	denotes	these
T13552	24647-24649	VBZ	denotes	is
T13546	24650-24660	VBN	denotes	maintained
T13553	24661-24670	RB	denotes	afterward
T13554	24670-24671	.	denotes	.
T13555	24671-24771	sentence	denotes	Note that RanBP2+/− mice lack the growth spur observed in wild-type mice between 3 and 4 mo of age.
T13556	24672-24676	VB	denotes	Note
T13557	24677-24681	IN	denotes	that
T13559	24682-24688	NN	denotes	RanBP2
T13561	24688-24689	SYM	denotes	+
T13562	24689-24690	HYPH	denotes	/
T13563	24690-24691	SYM	denotes	−
T13560	24692-24696	NNS	denotes	mice
T13558	24697-24701	VBP	denotes	lack
T13564	24702-24705	DT	denotes	the
T13566	24706-24712	NN	denotes	growth
T13565	24713-24717	NN	denotes	spur
T13567	24718-24726	VBN	denotes	observed
T13568	24727-24729	IN	denotes	in
T13569	24730-24734	JJ	denotes	wild
T13571	24734-24735	HYPH	denotes	-
T13570	24735-24739	NN	denotes	type
T13572	24740-24744	NNS	denotes	mice
T13573	24745-24752	IN	denotes	between
T13574	24753-24754	CD	denotes	3
T13576	24755-24758	CC	denotes	and
T13577	24759-24760	CD	denotes	4
T13575	24761-24763	NNS	denotes	mo
T13578	24764-24766	IN	denotes	of
T13579	24767-24770	NN	denotes	age
T13580	24770-24771	.	denotes	.
T13581	24771-24983	sentence	denotes	(B) In comparison to inbred RanBP2+/−mice (129Ola genetic background), the difference in body weight between RanBP2+/+ and RanBP2+/− mice is masked upon placing these on a mixed 129Ola/C57Bl6 genetic background.
T13582	24772-24773	-LRB-	denotes	(
T13583	24773-24774	LS	denotes	B
T13585	24774-24775	-RRB-	denotes	)
T13586	24776-24778	IN	denotes	In
T13587	24779-24789	NN	denotes	comparison
T13588	24790-24792	IN	denotes	to
T13589	24793-24799	JJ	denotes	inbred
T13591	24800-24806	NN	denotes	RanBP2
T13592	24806-24807	SYM	denotes	+
T13593	24807-24808	HYPH	denotes	/
T13594	24808-24809	SYM	denotes	−
T13590	24809-24813	NNS	denotes	mice
T13595	24814-24815	-LRB-	denotes	(
T13596	24815-24821	NN	denotes	129Ola
T13598	24822-24829	JJ	denotes	genetic
T13597	24830-24840	NN	denotes	background
T13599	24840-24841	-RRB-	denotes	)
T13600	24841-24843	,	denotes	,
T13601	24843-24846	DT	denotes	the
T13602	24847-24857	NN	denotes	difference
T13603	24858-24860	IN	denotes	in
T13604	24861-24865	NN	denotes	body
T13605	24866-24872	NN	denotes	weight
T13606	24873-24880	IN	denotes	between
T13607	24881-24887	NN	denotes	RanBP2
T13609	24887-24888	SYM	denotes	+
T13610	24888-24889	HYPH	denotes	/
T13611	24889-24890	SYM	denotes	+
T13612	24891-24894	CC	denotes	and
T13613	24895-24901	NN	denotes	RanBP2
T13614	24901-24902	SYM	denotes	+
T13615	24902-24903	HYPH	denotes	/
T13616	24903-24904	SYM	denotes	−
T13608	24905-24909	NNS	denotes	mice
T13617	24910-24912	VBZ	denotes	is
T13584	24913-24919	VBN	denotes	masked
T13618	24920-24924	IN	denotes	upon
T13619	24925-24932	VBG	denotes	placing
T13620	24933-24938	DT	denotes	these
T13621	24939-24941	IN	denotes	on
T13622	24942-24943	DT	denotes	a
T13624	24944-24949	JJ	denotes	mixed
T13625	24950-24956	NN	denotes	129Ola
T13627	24956-24957	HYPH	denotes	/
T13626	24957-24963	NN	denotes	C57Bl6
T13628	24964-24971	JJ	denotes	genetic
T13623	24972-24982	NN	denotes	background
T13629	24982-24983	.	denotes	.
T13630	24983-25065	sentence	denotes	(C) RanBP2+/+ and RanBP2+/−inbred mice exhibit similar rates of food consumption.
T13631	24984-24985	-LRB-	denotes	(
T13632	24985-24986	LS	denotes	C
T13634	24986-24987	-RRB-	denotes	)
T13635	24988-24994	NN	denotes	RanBP2
T13637	24994-24995	SYM	denotes	+
T13638	24995-24996	HYPH	denotes	/
T13639	24996-24997	SYM	denotes	+
T13640	24998-25001	CC	denotes	and
T13641	25002-25008	NN	denotes	RanBP2
T13642	25008-25009	SYM	denotes	+
T13643	25009-25010	HYPH	denotes	/
T13644	25010-25011	SYM	denotes	−
T13645	25011-25017	JJ	denotes	inbred
T13636	25018-25022	NNS	denotes	mice
T13633	25023-25030	VBP	denotes	exhibit
T13646	25031-25038	JJ	denotes	similar
T13647	25039-25044	NNS	denotes	rates
T13648	25045-25047	IN	denotes	of
T13649	25048-25052	NN	denotes	food
T13650	25053-25064	NN	denotes	consumption
T13651	25064-25065	.	denotes	.
T13652	25065-25143	sentence	denotes	Mice in (A), (B), and (C) were placed on a high-fat diet since birth (n = 5).
T13653	25066-25070	NNS	denotes	Mice
T13655	25071-25073	IN	denotes	in
T13656	25074-25075	-LRB-	denotes	(
T13657	25075-25076	NN	denotes	A
T13658	25076-25077	-RRB-	denotes	)
T13659	25077-25079	,	denotes	,
T13660	25079-25080	-LRB-	denotes	(
T13661	25080-25081	NN	denotes	B
T13662	25081-25082	-RRB-	denotes	)
T13663	25082-25084	,	denotes	,
T13664	25084-25087	CC	denotes	and
T13665	25088-25089	-LRB-	denotes	(
T13666	25089-25090	NN	denotes	C
T13667	25090-25091	-RRB-	denotes	)
T13668	25092-25096	VBD	denotes	were
T13654	25097-25103	VBN	denotes	placed
T13669	25104-25106	IN	denotes	on
T13670	25107-25108	DT	denotes	a
T13672	25109-25113	JJ	denotes	high
T13674	25113-25114	HYPH	denotes	-
T13673	25114-25117	NN	denotes	fat
T13671	25118-25122	NN	denotes	diet
T13675	25123-25128	IN	denotes	since
T13676	25129-25134	NN	denotes	birth
T13677	25135-25136	-LRB-	denotes	(
T13679	25136-25137	NN	denotes	n
T13680	25138-25139	SYM	denotes	=
T13678	25140-25141	CD	denotes	5
T13681	25141-25142	-RRB-	denotes	)
T13682	25142-25143	.	denotes	.
T4437	25144-25147	NN	denotes	HKI
T4439	25148-25150	IN	denotes	in
T4440	25151-25154	DT	denotes	the
T4441	25155-25158	NN	denotes	CNS
T4442	25159-25160	-LRB-	denotes	(
T4443	25160-25165	NN	denotes	brain
T4444	25166-25169	CC	denotes	and
T4445	25170-25176	NN	denotes	retina
T4446	25176-25177	-RRB-	denotes	)
T4438	25178-25186	VBZ	denotes	accounts
T4447	25187-25196	RB	denotes	virtually
T4448	25197-25200	IN	denotes	for
T4449	25201-25204	DT	denotes	all
T4450	25205-25215	NN	denotes	expression
T4451	25216-25218	IN	denotes	of
T4452	25219-25221	NN	denotes	HK
T4453	25222-25230	NNS	denotes	isozymes
T4454	25231-25234	CC	denotes	and
T4455	25235-25242	NN	denotes	glucose
T4456	25243-25254	NN	denotes	utilization
T4457	25255-25257	IN	denotes	in
T4458	25258-25261	DT	denotes	the
T4459	25262-25265	NN	denotes	CNS
T4460	25266-25267	-LRB-	denotes	[
T4462	25267-25269	CD	denotes	33
T4463	25269-25270	,	denotes	,
T4461	25270-25272	CD	denotes	34
T4464	25272-25273	-RRB-	denotes	]
T4465	25273-25274	.	denotes	.
T4466	25274-25571	sentence	denotes	Moreover, glucose is the sole reliance source of energy in the CNS under normal conditions, the CNS lacks glucose storage sources, and despite the disproportionate mass of the CNS to the rest of the body, the CNS consumes daily about 60% of the body's glucose and 25% of the total oxygen [35,36].
T4467	25275-25283	RB	denotes	Moreover
T4469	25283-25285	,	denotes	,
T4470	25285-25292	NN	denotes	glucose
T4468	25293-25295	VBZ	denotes	is
T4471	25296-25299	DT	denotes	the
T4473	25300-25304	JJ	denotes	sole
T4474	25305-25313	NN	denotes	reliance
T4472	25314-25320	NN	denotes	source
T4475	25321-25323	IN	denotes	of
T4476	25324-25330	NN	denotes	energy
T4477	25331-25333	IN	denotes	in
T4478	25334-25337	DT	denotes	the
T4479	25338-25341	NN	denotes	CNS
T4480	25342-25347	IN	denotes	under
T4481	25348-25354	JJ	denotes	normal
T4482	25355-25365	NNS	denotes	conditions
T4483	25365-25367	,	denotes	,
T4484	25367-25370	DT	denotes	the
T4485	25371-25374	NN	denotes	CNS
T4486	25375-25380	VBZ	denotes	lacks
T4487	25381-25388	NN	denotes	glucose
T4488	25389-25396	NN	denotes	storage
T4489	25397-25404	NNS	denotes	sources
T4490	25404-25406	,	denotes	,
T4491	25406-25409	CC	denotes	and
T4492	25410-25417	IN	denotes	despite
T4494	25418-25421	DT	denotes	the
T4496	25422-25438	JJ	denotes	disproportionate
T4495	25439-25443	NN	denotes	mass
T4497	25444-25446	IN	denotes	of
T4498	25447-25450	DT	denotes	the
T4499	25451-25454	NN	denotes	CNS
T4500	25455-25457	IN	denotes	to
T4501	25458-25461	DT	denotes	the
T4502	25462-25466	NN	denotes	rest
T4503	25467-25469	IN	denotes	of
T4504	25470-25473	DT	denotes	the
T4505	25474-25478	NN	denotes	body
T4506	25478-25480	,	denotes	,
T4507	25480-25483	DT	denotes	the
T4508	25484-25487	NN	denotes	CNS
T4493	25488-25496	VBZ	denotes	consumes
T4509	25497-25502	RB	denotes	daily
T4510	25503-25508	IN	denotes	about
T4511	25509-25511	CD	denotes	60
T4512	25511-25512	NN	denotes	%
T4513	25513-25515	IN	denotes	of
T4514	25516-25519	DT	denotes	the
T4515	25520-25524	NN	denotes	body
T4517	25524-25526	POS	denotes	's
T4516	25527-25534	NN	denotes	glucose
T4518	25535-25538	CC	denotes	and
T4519	25539-25541	CD	denotes	25
T4520	25541-25542	NN	denotes	%
T4521	25543-25545	IN	denotes	of
T4522	25546-25549	DT	denotes	the
T4524	25550-25555	JJ	denotes	total
T4523	25556-25562	NN	denotes	oxygen
T4525	25563-25564	-LRB-	denotes	[
T4527	25564-25566	CD	denotes	35
T4528	25566-25567	,	denotes	,
T4526	25567-25569	CD	denotes	36
T4529	25569-25570	-RRB-	denotes	]
T4530	25570-25571	.	denotes	.
T4531	25571-25723	sentence	denotes	To determine the impact of RanBP2 haploinsufficiency on the utilization, formation, and uptake of glucose, we carried out several physiological assays.
T4532	25572-25574	TO	denotes	To
T4533	25575-25584	VB	denotes	determine
T4535	25585-25588	DT	denotes	the
T4536	25589-25595	NN	denotes	impact
T4537	25596-25598	IN	denotes	of
T4538	25599-25605	NN	denotes	RanBP2
T4539	25606-25624	NN	denotes	haploinsufficiency
T4540	25625-25627	IN	denotes	on
T4541	25628-25631	DT	denotes	the
T4542	25632-25643	NN	denotes	utilization
T4543	25643-25645	,	denotes	,
T4544	25645-25654	NN	denotes	formation
T4545	25654-25656	,	denotes	,
T4546	25656-25659	CC	denotes	and
T4547	25660-25666	NN	denotes	uptake
T4548	25667-25669	IN	denotes	of
T4549	25670-25677	NN	denotes	glucose
T4550	25677-25679	,	denotes	,
T4551	25679-25681	PRP	denotes	we
T4534	25682-25689	VBD	denotes	carried
T4552	25690-25693	RP	denotes	out
T4553	25694-25701	JJ	denotes	several
T4555	25702-25715	JJ	denotes	physiological
T4554	25716-25722	NNS	denotes	assays
T4556	25722-25723	.	denotes	.
T4557	25723-26034	sentence	denotes	In contrast to mice placed on a normal chow diet (~5% fat; unpublished data), RanBP2+/−mice on a higher fat diet (~10% fat) performed significantly worse in the glucose tolerance test beginning at 6 mo of age (Figure 7A and 7B), thus supporting that the RanBP2+/− mice exhibited a deficit in glucose clearance.
T4558	25724-25726	IN	denotes	In
T4560	25727-25735	NN	denotes	contrast
T4561	25736-25738	IN	denotes	to
T4562	25739-25743	NNS	denotes	mice
T4563	25744-25750	VBN	denotes	placed
T4564	25751-25753	IN	denotes	on
T4565	25754-25755	DT	denotes	a
T4567	25756-25762	JJ	denotes	normal
T4568	25763-25767	NN	denotes	chow
T4566	25768-25772	NN	denotes	diet
T4569	25773-25774	-LRB-	denotes	(
T4571	25774-25775	SYM	denotes	~
T4572	25775-25776	CD	denotes	5
T4573	25776-25777	NN	denotes	%
T4574	25778-25781	NN	denotes	fat
T4575	25781-25782	:	denotes	;
T4576	25783-25794	JJ	denotes	unpublished
T4570	25795-25799	NNS	denotes	data
T4577	25799-25800	-RRB-	denotes	)
T4578	25800-25802	,	denotes	,
T4579	25802-25808	NN	denotes	RanBP2
T4581	25808-25809	SYM	denotes	+
T4582	25809-25810	HYPH	denotes	/
T4583	25810-25811	SYM	denotes	−
T4580	25811-25815	NNS	denotes	mice
T4584	25816-25818	IN	denotes	on
T4585	25819-25820	DT	denotes	a
T4587	25821-25827	JJR	denotes	higher
T4588	25828-25831	NN	denotes	fat
T4586	25832-25836	NN	denotes	diet
T4589	25837-25838	-LRB-	denotes	(
T4591	25838-25839	SYM	denotes	~
T4592	25839-25841	CD	denotes	10
T4593	25841-25842	NN	denotes	%
T4590	25843-25846	NN	denotes	fat
T4594	25846-25847	-RRB-	denotes	)
T4559	25848-25857	VBD	denotes	performed
T4595	25858-25871	RB	denotes	significantly
T4596	25872-25877	RBR	denotes	worse
T4597	25878-25880	IN	denotes	in
T4598	25881-25884	DT	denotes	the
T4600	25885-25892	NN	denotes	glucose
T4601	25893-25902	NN	denotes	tolerance
T4599	25903-25907	NN	denotes	test
T4602	25908-25917	VBG	denotes	beginning
T4603	25918-25920	IN	denotes	at
T4604	25921-25922	CD	denotes	6
T4605	25923-25925	NNS	denotes	mo
T4606	25926-25928	IN	denotes	of
T4607	25929-25932	NN	denotes	age
T4608	25933-25934	-LRB-	denotes	(
T4610	25934-25940	NN	denotes	Figure
T4609	25941-25943	NN	denotes	7A
T4611	25944-25947	CC	denotes	and
T4612	25948-25950	NN	denotes	7B
T4613	25950-25951	-RRB-	denotes	)
T4614	25951-25953	,	denotes	,
T4615	25953-25957	RB	denotes	thus
T4616	25958-25968	VBG	denotes	supporting
T4617	25969-25973	IN	denotes	that
T4619	25974-25977	DT	denotes	the
T4621	25978-25984	NN	denotes	RanBP2
T4622	25984-25985	SYM	denotes	+
T4623	25985-25986	HYPH	denotes	/
T4624	25986-25987	SYM	denotes	−
T4620	25988-25992	NNS	denotes	mice
T4618	25993-26002	VBD	denotes	exhibited
T4625	26003-26004	DT	denotes	a
T4626	26005-26012	NN	denotes	deficit
T4627	26013-26015	IN	denotes	in
T4628	26016-26023	NN	denotes	glucose
T4629	26024-26033	NN	denotes	clearance
T4630	26033-26034	.	denotes	.
T4631	26034-26126	sentence	denotes	This deficit was rescued in RanBP2+/− mice of mixed C57BL/6J/129Ola background (Figure S3).
T4632	26035-26039	DT	denotes	This
T4633	26040-26047	NN	denotes	deficit
T4635	26048-26051	VBD	denotes	was
T4634	26052-26059	VBN	denotes	rescued
T4636	26060-26062	IN	denotes	in
T4637	26063-26069	NN	denotes	RanBP2
T4639	26069-26070	SYM	denotes	+
T4640	26070-26071	HYPH	denotes	/
T4641	26071-26072	SYM	denotes	−
T4638	26073-26077	NNS	denotes	mice
T4642	26078-26080	IN	denotes	of
T4643	26081-26086	VBN	denotes	mixed
T4645	26087-26092	NN	denotes	C57BL
T4647	26092-26093	HYPH	denotes	/
T4648	26093-26095	NN	denotes	6J
T4649	26095-26096	HYPH	denotes	/
T4646	26096-26102	NN	denotes	129Ola
T4644	26103-26113	NN	denotes	background
T4650	26114-26115	-LRB-	denotes	(
T4652	26115-26121	NN	denotes	Figure
T4651	26122-26124	NN	denotes	S3
T4653	26124-26125	-RRB-	denotes	)
T4654	26125-26126	.	denotes	.
T4655	26126-26230	sentence	denotes	Glucose clearance was not affected due to a disturbance in insulin-mediated glucose uptake (Figure 7C).
T4656	26127-26134	NN	denotes	Glucose
T4657	26135-26144	NN	denotes	clearance
T4659	26145-26148	VBD	denotes	was
T4660	26149-26152	RB	denotes	not
T4658	26153-26161	VBN	denotes	affected
T4661	26162-26165	IN	denotes	due
T4662	26166-26168	IN	denotes	to
T4663	26169-26170	DT	denotes	a
T4664	26171-26182	NN	denotes	disturbance
T4665	26183-26185	IN	denotes	in
T4666	26186-26193	NN	denotes	insulin
T4668	26193-26194	HYPH	denotes	-
T4667	26194-26202	JJ	denotes	mediated
T4670	26203-26210	NN	denotes	glucose
T4669	26211-26217	NN	denotes	uptake
T4671	26218-26219	-LRB-	denotes	(
T4673	26219-26225	NN	denotes	Figure
T4672	26226-26228	NN	denotes	7C
T4674	26228-26229	-RRB-	denotes	)
T4675	26229-26230	.	denotes	.
T4676	26230-26386	sentence	denotes	Then, we probed whether RanBP2 induces impairment of gluconeogenesis, which could contribute to the pathophysiological production and clearance of glucose.
T4677	26231-26235	RB	denotes	Then
T4679	26235-26237	,	denotes	,
T4680	26237-26239	PRP	denotes	we
T4678	26240-26246	VBD	denotes	probed
T4681	26247-26254	IN	denotes	whether
T4683	26255-26261	NN	denotes	RanBP2
T4682	26262-26269	VBZ	denotes	induces
T4684	26270-26280	NN	denotes	impairment
T4685	26281-26283	IN	denotes	of
T4686	26284-26299	NN	denotes	gluconeogenesis
T4687	26299-26301	,	denotes	,
T4688	26301-26306	WDT	denotes	which
T4690	26307-26312	MD	denotes	could
T4689	26313-26323	VB	denotes	contribute
T4691	26324-26326	IN	denotes	to
T4692	26327-26330	DT	denotes	the
T4694	26331-26349	JJ	denotes	pathophysiological
T4693	26350-26360	NN	denotes	production
T4695	26361-26364	CC	denotes	and
T4696	26365-26374	NN	denotes	clearance
T4697	26375-26377	IN	denotes	of
T4698	26378-26385	NN	denotes	glucose
T4699	26385-26386	.	denotes	.
T4700	26386-26585	sentence	denotes	To this end, the administration of the gluconeogenic substrate precursor, pyruvate (pyruvate tolerance test), showed that there was no difference in glucose production in RanBP2+/− mice (Figure 7D).
T4701	26387-26389	IN	denotes	To
T4703	26390-26394	DT	denotes	this
T4704	26395-26398	NN	denotes	end
T4705	26398-26400	,	denotes	,
T4706	26400-26403	DT	denotes	the
T4707	26404-26418	NN	denotes	administration
T4708	26419-26421	IN	denotes	of
T4709	26422-26425	DT	denotes	the
T4711	26426-26439	JJ	denotes	gluconeogenic
T4712	26440-26449	NN	denotes	substrate
T4710	26450-26459	NN	denotes	precursor
T4713	26459-26461	,	denotes	,
T4714	26461-26469	NN	denotes	pyruvate
T4715	26470-26471	-LRB-	denotes	(
T4716	26471-26479	NN	denotes	pyruvate
T4717	26480-26489	NN	denotes	tolerance
T4718	26490-26494	NN	denotes	test
T4719	26494-26495	-RRB-	denotes	)
T4720	26495-26497	,	denotes	,
T4702	26497-26503	VBD	denotes	showed
T4721	26504-26508	IN	denotes	that
T4723	26509-26514	EX	denotes	there
T4722	26515-26518	VBD	denotes	was
T4724	26519-26521	DT	denotes	no
T4725	26522-26532	NN	denotes	difference
T4726	26533-26535	IN	denotes	in
T4727	26536-26543	NN	denotes	glucose
T4728	26544-26554	NN	denotes	production
T4729	26555-26557	IN	denotes	in
T4730	26558-26564	NN	denotes	RanBP2
T4732	26564-26565	SYM	denotes	+
T4733	26565-26566	HYPH	denotes	/
T4734	26566-26567	SYM	denotes	−
T4731	26568-26572	NNS	denotes	mice
T4735	26573-26574	-LRB-	denotes	(
T4737	26574-26580	NN	denotes	Figure
T4736	26581-26583	NN	denotes	7D
T4738	26583-26584	-RRB-	denotes	)
T4739	26584-26585	.	denotes	.
T4740	26585-26673	sentence	denotes	Hence, partial loss-of-function of RanBP2 had no impact on the gluconeogenesis pathway.
T4741	26586-26591	RB	denotes	Hence
T4743	26591-26593	,	denotes	,
T4744	26593-26600	JJ	denotes	partial
T4745	26601-26605	NN	denotes	loss
T4746	26605-26606	HYPH	denotes	-
T4747	26606-26608	IN	denotes	of
T4748	26608-26609	HYPH	denotes	-
T4749	26609-26617	NN	denotes	function
T4750	26618-26620	IN	denotes	of
T4751	26621-26627	NN	denotes	RanBP2
T4742	26628-26631	VBD	denotes	had
T4752	26632-26634	DT	denotes	no
T4753	26635-26641	NN	denotes	impact
T4754	26642-26644	IN	denotes	on
T4755	26645-26648	DT	denotes	the
T4757	26649-26664	NN	denotes	gluconeogenesis
T4756	26665-26672	NN	denotes	pathway
T4758	26672-26673	.	denotes	.
T4759	26673-26875	sentence	denotes	However, upon glucose production (15 min), the clearance rates of glucose were again significantly slower in RanBP2+/− than in RanBP2+/+ mice (Figure 7D), confirming an impairment in glucose breakdown.
T4760	26674-26681	RB	denotes	However
T4762	26681-26683	,	denotes	,
T4763	26683-26687	IN	denotes	upon
T4764	26688-26695	NN	denotes	glucose
T4765	26696-26706	NN	denotes	production
T4766	26707-26708	-LRB-	denotes	(
T4768	26708-26710	CD	denotes	15
T4767	26711-26714	NNS	denotes	min
T4769	26714-26715	-RRB-	denotes	)
T4770	26715-26717	,	denotes	,
T4771	26717-26720	DT	denotes	the
T4773	26721-26730	NN	denotes	clearance
T4772	26731-26736	NNS	denotes	rates
T4774	26737-26739	IN	denotes	of
T4775	26740-26747	NN	denotes	glucose
T4761	26748-26752	VBD	denotes	were
T4776	26753-26758	RB	denotes	again
T4777	26759-26772	RB	denotes	significantly
T4778	26773-26779	JJR	denotes	slower
T4779	26780-26782	IN	denotes	in
T4780	26783-26789	NN	denotes	RanBP2
T4781	26789-26790	SYM	denotes	+
T4782	26790-26791	HYPH	denotes	/
T4783	26791-26792	SYM	denotes	−
T4784	26793-26797	IN	denotes	than
T4785	26798-26800	IN	denotes	in
T4786	26801-26807	NN	denotes	RanBP2
T4788	26807-26808	SYM	denotes	+
T4789	26808-26809	HYPH	denotes	/
T4790	26809-26810	SYM	denotes	+
T4787	26811-26815	NNS	denotes	mice
T4791	26816-26817	-LRB-	denotes	(
T4793	26817-26823	NN	denotes	Figure
T4792	26824-26826	NN	denotes	7D
T4794	26826-26827	-RRB-	denotes	)
T4795	26827-26829	,	denotes	,
T4796	26829-26839	VBG	denotes	confirming
T4797	26840-26842	DT	denotes	an
T4798	26843-26853	NN	denotes	impairment
T4799	26854-26856	IN	denotes	in
T4800	26857-26864	NN	denotes	glucose
T4801	26865-26874	NN	denotes	breakdown
T4802	26874-26875	.	denotes	.
T13772	26886-26895	JJ	denotes	Metabolic
T13773	26896-26906	NNS	denotes	Phenotypes
T13774	26907-26909	IN	denotes	of
T13775	26910-26916	NN	denotes	RanBP2
T13777	26916-26917	SYM	denotes	+
T13778	26917-26918	HYPH	denotes	/
T13779	26918-26919	SYM	denotes	−
T13780	26920-26926	JJ	denotes	Inbred
T13776	26927-26931	NNS	denotes	Mice
T13781	26932-26934	IN	denotes	on
T13782	26935-26939	JJ	denotes	High
T13784	26939-26940	HYPH	denotes	-
T13783	26940-26943	NN	denotes	Fat
T13785	26944-26948	NN	denotes	Diet
T13786	26948-27073	sentence	denotes	(A) 3-mo-old inbred RanBP2+/− mice (n = 5) have normal glucose clearance rates upon glucose challenge and overnight fasting.
T13787	26949-26950	-LRB-	denotes	(
T13788	26950-26951	LS	denotes	A
T13790	26951-26952	-RRB-	denotes	)
T13791	26953-26954	CD	denotes	3
T13793	26954-26955	HYPH	denotes	-
T13792	26955-26957	NN	denotes	mo
T13795	26957-26958	HYPH	denotes	-
T13794	26958-26961	JJ	denotes	old
T13797	26962-26968	JJ	denotes	inbred
T13798	26969-26975	NN	denotes	RanBP2
T13799	26975-26976	SYM	denotes	+
T13800	26976-26977	HYPH	denotes	/
T13801	26977-26978	SYM	denotes	−
T13796	26979-26983	NNS	denotes	mice
T13802	26984-26985	-LRB-	denotes	(
T13804	26985-26986	NN	denotes	n
T13805	26987-26988	SYM	denotes	=
T13803	26989-26990	CD	denotes	5
T13806	26990-26991	-RRB-	denotes	)
T13789	26992-26996	VBP	denotes	have
T13807	26997-27003	JJ	denotes	normal
T13809	27004-27011	NN	denotes	glucose
T13810	27012-27021	NN	denotes	clearance
T13808	27022-27027	NNS	denotes	rates
T13811	27028-27032	IN	denotes	upon
T13812	27033-27040	NN	denotes	glucose
T13813	27041-27050	NN	denotes	challenge
T13814	27051-27054	CC	denotes	and
T13815	27055-27064	JJ	denotes	overnight
T13816	27065-27072	NN	denotes	fasting
T13817	27072-27073	.	denotes	.
T13818	27073-27228	sentence	denotes	(B) In contrast, 6-mo-old inbred RanBP2+/− mice (n = 5) have significantly decreased glucose clearance rates upon glucose challenge and overnight fasting.
T13819	27074-27075	-LRB-	denotes	(
T13820	27075-27076	LS	denotes	B
T13822	27076-27077	-RRB-	denotes	)
T13823	27078-27080	IN	denotes	In
T13824	27081-27089	NN	denotes	contrast
T13825	27089-27091	,	denotes	,
T13826	27091-27092	CD	denotes	6
T13828	27092-27093	HYPH	denotes	-
T13827	27093-27095	NN	denotes	mo
T13830	27095-27096	HYPH	denotes	-
T13829	27096-27099	JJ	denotes	old
T13832	27100-27106	JJ	denotes	inbred
T13833	27107-27113	NN	denotes	RanBP2
T13834	27113-27114	SYM	denotes	+
T13835	27114-27115	HYPH	denotes	/
T13836	27115-27116	SYM	denotes	−
T13831	27117-27121	NNS	denotes	mice
T13837	27122-27123	-LRB-	denotes	(
T13839	27123-27124	NN	denotes	n
T13840	27125-27126	SYM	denotes	=
T13838	27127-27128	CD	denotes	5
T13841	27128-27129	-RRB-	denotes	)
T13821	27130-27134	VBP	denotes	have
T13842	27135-27148	RB	denotes	significantly
T13843	27149-27158	VBN	denotes	decreased
T13845	27159-27166	NN	denotes	glucose
T13846	27167-27176	NN	denotes	clearance
T13844	27177-27182	NNS	denotes	rates
T13847	27183-27187	IN	denotes	upon
T13848	27188-27195	NN	denotes	glucose
T13849	27196-27205	NN	denotes	challenge
T13850	27206-27209	CC	denotes	and
T13851	27210-27219	JJ	denotes	overnight
T13852	27220-27227	NN	denotes	fasting
T13853	27227-27228	.	denotes	.
T13854	27228-27383	sentence	denotes	(C) Fasted 6- to 8-mo-old RanBP2+/+ and RanBP2+/− mice have no difference in insulin-mediated glucose uptake as assayed by insulin tolerance test (n = 5).
T13855	27229-27230	-LRB-	denotes	(
T13856	27230-27231	LS	denotes	C
T13858	27231-27232	-RRB-	denotes	)
T13859	27233-27239	VBN	denotes	Fasted
T13861	27240-27241	CD	denotes	6
T13863	27241-27242	HYPH	denotes	-
T13864	27243-27245	IN	denotes	to
T13862	27246-27247	CD	denotes	8
T13866	27247-27248	HYPH	denotes	-
T13865	27248-27250	NN	denotes	mo
T13868	27250-27251	HYPH	denotes	-
T13867	27251-27254	JJ	denotes	old
T13869	27255-27261	NN	denotes	RanBP2
T13870	27261-27262	SYM	denotes	+
T13871	27262-27263	HYPH	denotes	/
T13872	27263-27264	SYM	denotes	+
T13873	27265-27268	CC	denotes	and
T13874	27269-27275	NN	denotes	RanBP2
T13875	27275-27276	SYM	denotes	+
T13876	27276-27277	HYPH	denotes	/
T13877	27277-27278	SYM	denotes	−
T13860	27279-27283	NNS	denotes	mice
T13857	27284-27288	VBP	denotes	have
T13878	27289-27291	DT	denotes	no
T13879	27292-27302	NN	denotes	difference
T13880	27303-27305	IN	denotes	in
T13881	27306-27313	NN	denotes	insulin
T13883	27313-27314	HYPH	denotes	-
T13882	27314-27322	JJ	denotes	mediated
T13885	27323-27330	NN	denotes	glucose
T13884	27331-27337	NN	denotes	uptake
T13886	27338-27340	IN	denotes	as
T13887	27341-27348	VBN	denotes	assayed
T13888	27349-27351	IN	denotes	by
T13889	27352-27359	NN	denotes	insulin
T13890	27360-27369	NN	denotes	tolerance
T13891	27370-27374	NN	denotes	test
T13892	27375-27376	-LRB-	denotes	(
T13894	27376-27377	NN	denotes	n
T13895	27378-27379	SYM	denotes	=
T13893	27380-27381	CD	denotes	5
T13896	27381-27382	-RRB-	denotes	)
T13897	27382-27383	.	denotes	.
T13898	27383-27525	sentence	denotes	(D) Pyruvate tolerance test shows normal rise in glucose but decreased glucose clearance between inbred RanBP2+/+ and RanBP2+/− mice (n = 5).
T13899	27384-27385	-LRB-	denotes	(
T13900	27385-27386	LS	denotes	D
T13902	27386-27387	-RRB-	denotes	)
T13903	27388-27396	NN	denotes	Pyruvate
T13904	27397-27406	NN	denotes	tolerance
T13905	27407-27411	NN	denotes	test
T13901	27412-27417	VBZ	denotes	shows
T13906	27418-27424	JJ	denotes	normal
T13907	27425-27429	NN	denotes	rise
T13908	27430-27432	IN	denotes	in
T13909	27433-27440	NN	denotes	glucose
T13910	27441-27444	CC	denotes	but
T13911	27445-27454	VBN	denotes	decreased
T13913	27455-27462	NN	denotes	glucose
T13912	27463-27472	NN	denotes	clearance
T13914	27473-27480	IN	denotes	between
T13915	27481-27487	JJ	denotes	inbred
T13917	27488-27494	NN	denotes	RanBP2
T13918	27494-27495	SYM	denotes	+
T13919	27495-27496	HYPH	denotes	/
T13920	27496-27497	SYM	denotes	+
T13921	27498-27501	CC	denotes	and
T13922	27502-27508	NN	denotes	RanBP2
T13923	27508-27509	SYM	denotes	+
T13924	27509-27510	HYPH	denotes	/
T13925	27510-27511	SYM	denotes	−
T13916	27512-27516	NNS	denotes	mice
T13926	27517-27518	-LRB-	denotes	(
T13928	27518-27519	NN	denotes	n
T13929	27520-27521	SYM	denotes	=
T13927	27522-27523	CD	denotes	5
T13930	27523-27524	-RRB-	denotes	)
T13931	27524-27525	.	denotes	.
T5002	27527-27545	NN	denotes	Haploinsufficiency
T5004	27546-27548	IN	denotes	of
T5005	27549-27555	NN	denotes	RanBP2
T5003	27556-27562	VBZ	denotes	Causes
T5006	27563-27571	NNS	denotes	Deficits
T5007	27572-27574	IN	denotes	in
T5008	27575-27578	DT	denotes	the
T5010	27579-27599	JJ	denotes	Electrophysiological
T5009	27600-27606	NN	denotes	Output
T5011	27607-27609	IN	denotes	of
T5012	27610-27620	JJ	denotes	Receptoral
T5014	27621-27624	CC	denotes	and
T5015	27625-27639	JJ	denotes	Postreceptoral
T5016	27640-27647	JJ	denotes	Retinal
T5013	27648-27655	NNS	denotes	Neurons
T5017	27655-28188	sentence	denotes	In light of the prominent expression of RanBP2 and HKI in retinal neurons [1,19], the vital dependence of the neuronal retina (and brain) on glucose as the main substrate source for energy production, and the determinant impact of metabolic disorders, such as diabetes, in retinal function (e.g., diabetic retinopathy) [37], we probed the impact of deficits in RanBP2, HKI, and ATP, on the electrophysiological responses of subclasses (rod and cone) photoreceptor and postreceptor retinal neurons of RanBP2+/− and in RanBP2+/+ mice.
T5018	27656-27658	IN	denotes	In
T5020	27659-27664	NN	denotes	light
T5021	27665-27667	IN	denotes	of
T5022	27668-27671	DT	denotes	the
T5024	27672-27681	JJ	denotes	prominent
T5023	27682-27692	NN	denotes	expression
T5025	27693-27695	IN	denotes	of
T5026	27696-27702	NN	denotes	RanBP2
T5027	27703-27706	CC	denotes	and
T5028	27707-27710	NN	denotes	HKI
T5029	27711-27713	IN	denotes	in
T5030	27714-27721	JJ	denotes	retinal
T5031	27722-27729	NNS	denotes	neurons
T5032	27730-27731	-LRB-	denotes	[
T5034	27731-27732	CD	denotes	1
T5035	27732-27733	,	denotes	,
T5033	27733-27735	CD	denotes	19
T5036	27735-27736	-RRB-	denotes	]
T5037	27736-27738	,	denotes	,
T5038	27738-27741	DT	denotes	the
T5040	27742-27747	JJ	denotes	vital
T5039	27748-27758	NN	denotes	dependence
T5041	27759-27761	IN	denotes	of
T5042	27762-27765	DT	denotes	the
T5044	27766-27774	JJ	denotes	neuronal
T5043	27775-27781	NN	denotes	retina
T5045	27782-27783	-LRB-	denotes	(
T5046	27783-27786	CC	denotes	and
T5047	27787-27792	NN	denotes	brain
T5048	27792-27793	-RRB-	denotes	)
T5049	27794-27796	IN	denotes	on
T5050	27797-27804	NN	denotes	glucose
T5051	27805-27807	IN	denotes	as
T5052	27808-27811	DT	denotes	the
T5054	27812-27816	JJ	denotes	main
T5055	27817-27826	NN	denotes	substrate
T5053	27827-27833	NN	denotes	source
T5056	27834-27837	IN	denotes	for
T5057	27838-27844	NN	denotes	energy
T5058	27845-27855	NN	denotes	production
T5059	27855-27857	,	denotes	,
T5060	27857-27860	CC	denotes	and
T5061	27861-27864	DT	denotes	the
T5063	27865-27876	JJ	denotes	determinant
T5062	27877-27883	NN	denotes	impact
T5064	27884-27886	IN	denotes	of
T5065	27887-27896	JJ	denotes	metabolic
T5066	27897-27906	NNS	denotes	disorders
T5067	27906-27908	,	denotes	,
T5068	27908-27912	IN	denotes	such
T5069	27913-27915	IN	denotes	as
T5070	27916-27924	NN	denotes	diabetes
T5071	27924-27926	,	denotes	,
T5072	27926-27928	IN	denotes	in
T5073	27929-27936	JJ	denotes	retinal
T5074	27937-27945	NN	denotes	function
T5075	27946-27947	-LRB-	denotes	(
T5076	27947-27951	FW	denotes	e.g.
T5078	27951-27953	,	denotes	,
T5079	27953-27961	JJ	denotes	diabetic
T5077	27962-27973	NN	denotes	retinopathy
T5080	27973-27974	-RRB-	denotes	)
T5081	27975-27976	-LRB-	denotes	[
T5082	27976-27978	CD	denotes	37
T5083	27978-27979	-RRB-	denotes	]
T5084	27979-27981	,	denotes	,
T5085	27981-27983	PRP	denotes	we
T5019	27984-27990	VBD	denotes	probed
T5086	27991-27994	DT	denotes	the
T5087	27995-28001	NN	denotes	impact
T5088	28002-28004	IN	denotes	of
T5089	28005-28013	NNS	denotes	deficits
T5090	28014-28016	IN	denotes	in
T5091	28017-28023	NN	denotes	RanBP2
T5092	28023-28025	,	denotes	,
T5093	28025-28028	NN	denotes	HKI
T5094	28028-28030	,	denotes	,
T5095	28030-28033	CC	denotes	and
T5096	28034-28037	NN	denotes	ATP
T5097	28037-28039	,	denotes	,
T5098	28039-28041	IN	denotes	on
T5099	28042-28045	DT	denotes	the
T5101	28046-28066	JJ	denotes	electrophysiological
T5100	28067-28076	NNS	denotes	responses
T5102	28077-28079	IN	denotes	of
T5103	28080-28090	NNS	denotes	subclasses
T5105	28091-28092	-LRB-	denotes	(
T5106	28092-28095	NN	denotes	rod
T5107	28096-28099	CC	denotes	and
T5108	28100-28104	NN	denotes	cone
T5109	28104-28105	-RRB-	denotes	)
T5110	28106-28119	NN	denotes	photoreceptor
T5111	28120-28123	CC	denotes	and
T5112	28124-28136	NN	denotes	postreceptor
T5113	28137-28144	JJ	denotes	retinal
T5104	28145-28152	NNS	denotes	neurons
T5114	28153-28155	IN	denotes	of
T5115	28156-28162	NN	denotes	RanBP2
T5116	28162-28163	SYM	denotes	+
T5117	28163-28164	HYPH	denotes	/
T5118	28164-28165	SYM	denotes	−
T5119	28166-28169	CC	denotes	and
T5120	28170-28172	IN	denotes	in
T5121	28173-28179	NN	denotes	RanBP2
T5123	28179-28180	SYM	denotes	+
T5124	28180-28181	HYPH	denotes	/
T5125	28181-28182	SYM	denotes	+
T5122	28183-28187	NNS	denotes	mice
T5126	28187-28188	.	denotes	.
T5127	28188-28415	sentence	denotes	The scotopic (dark-adapted) responses mediated by the rod photoreceptor pathway at low-stimulus intensities and mixed rod and cone pathways at high-stimulus intensities were substantially reduced in RanBP2+/− mice (Figure 8A).
T5128	28189-28192	DT	denotes	The
T5130	28193-28201	JJ	denotes	scotopic
T5131	28202-28203	-LRB-	denotes	(
T5133	28203-28207	NN	denotes	dark
T5134	28207-28208	HYPH	denotes	-
T5132	28208-28215	VBN	denotes	adapted
T5135	28215-28216	-RRB-	denotes	)
T5129	28217-28226	NNS	denotes	responses
T5137	28227-28235	VBN	denotes	mediated
T5138	28236-28238	IN	denotes	by
T5139	28239-28242	DT	denotes	the
T5141	28243-28246	NN	denotes	rod
T5142	28247-28260	NN	denotes	photoreceptor
T5140	28261-28268	NN	denotes	pathway
T5143	28269-28271	IN	denotes	at
T5144	28272-28275	JJ	denotes	low
T5146	28275-28276	HYPH	denotes	-
T5145	28276-28284	NN	denotes	stimulus
T5147	28285-28296	NNS	denotes	intensities
T5148	28297-28300	CC	denotes	and
T5149	28301-28306	JJ	denotes	mixed
T5151	28307-28310	NN	denotes	rod
T5152	28311-28314	CC	denotes	and
T5153	28315-28319	NN	denotes	cone
T5150	28320-28328	NNS	denotes	pathways
T5154	28329-28331	IN	denotes	at
T5155	28332-28336	JJ	denotes	high
T5157	28336-28337	HYPH	denotes	-
T5156	28337-28345	NN	denotes	stimulus
T5158	28346-28357	NNS	denotes	intensities
T5159	28358-28362	VBD	denotes	were
T5160	28363-28376	RB	denotes	substantially
T5136	28377-28384	VBN	denotes	reduced
T5161	28385-28387	IN	denotes	in
T5162	28388-28394	NN	denotes	RanBP2
T5164	28394-28395	SYM	denotes	+
T5165	28395-28396	HYPH	denotes	/
T5166	28396-28397	SYM	denotes	−
T5163	28398-28402	NNS	denotes	mice
T5167	28403-28404	-LRB-	denotes	(
T5169	28404-28410	NN	denotes	Figure
T5168	28411-28413	NN	denotes	8A
T5170	28413-28414	-RRB-	denotes	)
T5171	28414-28415	.	denotes	.
T5172	28415-28720	sentence	denotes	The differences in the photopic (light-adapted) responses, initiated by cone photoreceptors, which make up 3% of the photosensory neurons in the mouse retina [38], were less obvious but still exhibited a trend toward reduced amplitudes across a range of increasing light stimulus intensities (Figure 8B).
T5173	28416-28419	DT	denotes	The
T5174	28420-28431	NNS	denotes	differences
T5176	28432-28434	IN	denotes	in
T5177	28435-28438	DT	denotes	the
T5179	28439-28447	JJ	denotes	photopic
T5180	28448-28449	-LRB-	denotes	(
T5182	28449-28454	NN	denotes	light
T5183	28454-28455	HYPH	denotes	-
T5181	28455-28462	VBN	denotes	adapted
T5184	28462-28463	-RRB-	denotes	)
T5178	28464-28473	NNS	denotes	responses
T5185	28473-28475	,	denotes	,
T5186	28475-28484	VBN	denotes	initiated
T5187	28485-28487	IN	denotes	by
T5188	28488-28492	NN	denotes	cone
T5189	28493-28507	NNS	denotes	photoreceptors
T5190	28507-28509	,	denotes	,
T5191	28509-28514	WDT	denotes	which
T5192	28515-28519	VBP	denotes	make
T5193	28520-28522	RP	denotes	up
T5194	28523-28524	CD	denotes	3
T5195	28524-28525	NN	denotes	%
T5196	28526-28528	IN	denotes	of
T5197	28529-28532	DT	denotes	the
T5199	28533-28545	JJ	denotes	photosensory
T5198	28546-28553	NNS	denotes	neurons
T5200	28554-28556	IN	denotes	in
T5201	28557-28560	DT	denotes	the
T5203	28561-28566	NN	denotes	mouse
T5202	28567-28573	NN	denotes	retina
T5204	28574-28575	-LRB-	denotes	[
T5205	28575-28577	CD	denotes	38
T5206	28577-28578	-RRB-	denotes	]
T5207	28578-28580	,	denotes	,
T5175	28580-28584	VBD	denotes	were
T5208	28585-28589	RBR	denotes	less
T5209	28590-28597	JJ	denotes	obvious
T5210	28598-28601	CC	denotes	but
T5211	28602-28607	RB	denotes	still
T5212	28608-28617	VBD	denotes	exhibited
T5213	28618-28619	DT	denotes	a
T5214	28620-28625	NN	denotes	trend
T5215	28626-28632	IN	denotes	toward
T5216	28633-28640	VBN	denotes	reduced
T5217	28641-28651	NNS	denotes	amplitudes
T5218	28652-28658	IN	denotes	across
T5219	28659-28660	DT	denotes	a
T5220	28661-28666	NN	denotes	range
T5221	28667-28669	IN	denotes	of
T5222	28670-28680	VBG	denotes	increasing
T5224	28681-28686	JJ	denotes	light
T5225	28687-28695	NN	denotes	stimulus
T5223	28696-28707	NNS	denotes	intensities
T5226	28708-28709	-LRB-	denotes	(
T5228	28709-28715	NN	denotes	Figure
T5227	28716-28718	NN	denotes	8B
T5229	28718-28719	-RRB-	denotes	)
T5230	28719-28720	.	denotes	.
T5231	28720-28905	sentence	denotes	The reduction in the scotopic responses included decreases in both b-wave (Figure 8C) and a-wave (Figure 8D) amplitudes mediated by postreceptoral and receptoral neurons, respectively.
T5232	28721-28724	DT	denotes	The
T5233	28725-28734	NN	denotes	reduction
T5235	28735-28737	IN	denotes	in
T5236	28738-28741	DT	denotes	the
T5238	28742-28750	JJ	denotes	scotopic
T5237	28751-28760	NNS	denotes	responses
T5234	28761-28769	VBD	denotes	included
T5239	28770-28779	NNS	denotes	decreases
T5240	28780-28782	IN	denotes	in
T5241	28783-28787	CC	denotes	both
T5243	28788-28789	NN	denotes	b
T5244	28789-28790	HYPH	denotes	-
T5242	28790-28794	NN	denotes	wave
T5246	28795-28796	-LRB-	denotes	(
T5248	28796-28802	NN	denotes	Figure
T5247	28803-28805	NN	denotes	8C
T5249	28805-28806	-RRB-	denotes	)
T5250	28807-28810	CC	denotes	and
T5251	28811-28812	NN	denotes	a
T5253	28812-28813	HYPH	denotes	-
T5252	28813-28817	NN	denotes	wave
T5254	28818-28819	-LRB-	denotes	(
T5256	28819-28825	NN	denotes	Figure
T5255	28826-28828	NN	denotes	8D
T5257	28828-28829	-RRB-	denotes	)
T5245	28830-28840	NNS	denotes	amplitudes
T5258	28841-28849	VBN	denotes	mediated
T5259	28850-28852	IN	denotes	by
T5260	28853-28867	JJ	denotes	postreceptoral
T5262	28868-28871	CC	denotes	and
T5263	28872-28882	JJ	denotes	receptoral
T5261	28883-28890	NNS	denotes	neurons
T5264	28890-28892	,	denotes	,
T5265	28892-28904	RB	denotes	respectively
T5266	28904-28905	.	denotes	.
T5267	28905-29100	sentence	denotes	Postreceptoral second-order neuron responses, represented by the b-wave, tended to be more consistently and substantially reduced than the a-waves, which directly reflect photoreceptor activity.
T5268	28906-28920	JJ	denotes	Postreceptoral
T5270	28921-28927	JJ	denotes	second
T5272	28927-28928	HYPH	denotes	-
T5271	28928-28933	NN	denotes	order
T5273	28934-28940	NN	denotes	neuron
T5269	28941-28950	NNS	denotes	responses
T5275	28950-28952	,	denotes	,
T5276	28952-28963	VBN	denotes	represented
T5277	28964-28966	IN	denotes	by
T5278	28967-28970	DT	denotes	the
T5280	28971-28972	NN	denotes	b
T5281	28972-28973	HYPH	denotes	-
T5279	28973-28977	NN	denotes	wave
T5282	28977-28979	,	denotes	,
T5274	28979-28985	VBD	denotes	tended
T5283	28986-28988	TO	denotes	to
T5284	28989-28991	VB	denotes	be
T5285	28992-28996	RBR	denotes	more
T5287	28997-29009	RB	denotes	consistently
T5288	29010-29013	CC	denotes	and
T5289	29014-29027	RB	denotes	substantially
T5286	29028-29035	VBN	denotes	reduced
T5290	29036-29040	IN	denotes	than
T5291	29041-29044	DT	denotes	the
T5293	29045-29046	NN	denotes	a
T5294	29046-29047	HYPH	denotes	-
T5292	29047-29052	NNS	denotes	waves
T5295	29052-29054	,	denotes	,
T5296	29054-29059	WDT	denotes	which
T5298	29060-29068	RB	denotes	directly
T5297	29069-29076	VBP	denotes	reflect
T5299	29077-29090	NN	denotes	photoreceptor
T5300	29091-29099	NN	denotes	activity
T5301	29099-29100	.	denotes	.
T5302	29100-29339	sentence	denotes	Since second-order neuron responses depend on input from photoreceptors, this suggests that reduced b-wave amplitudes are the result of the accumulation of decreases in the light response of both photoreceptors and postreceptoral neurons.
T5303	29101-29106	IN	denotes	Since
T5305	29107-29113	JJ	denotes	second
T5307	29113-29114	HYPH	denotes	-
T5306	29114-29119	NN	denotes	order
T5309	29120-29126	NN	denotes	neuron
T5308	29127-29136	NNS	denotes	responses
T5304	29137-29143	VBP	denotes	depend
T5311	29144-29146	IN	denotes	on
T5312	29147-29152	NN	denotes	input
T5313	29153-29157	IN	denotes	from
T5314	29158-29172	NNS	denotes	photoreceptors
T5315	29172-29174	,	denotes	,
T5316	29174-29178	DT	denotes	this
T5310	29179-29187	VBZ	denotes	suggests
T5317	29188-29192	IN	denotes	that
T5319	29193-29200	VBN	denotes	reduced
T5321	29201-29202	NN	denotes	b
T5323	29202-29203	HYPH	denotes	-
T5322	29203-29207	NN	denotes	wave
T5320	29208-29218	NNS	denotes	amplitudes
T5318	29219-29222	VBP	denotes	are
T5324	29223-29226	DT	denotes	the
T5325	29227-29233	NN	denotes	result
T5326	29234-29236	IN	denotes	of
T5327	29237-29240	DT	denotes	the
T5328	29241-29253	NN	denotes	accumulation
T5329	29254-29256	IN	denotes	of
T5330	29257-29266	NNS	denotes	decreases
T5331	29267-29269	IN	denotes	in
T5332	29270-29273	DT	denotes	the
T5334	29274-29279	JJ	denotes	light
T5333	29280-29288	NN	denotes	response
T5335	29289-29291	IN	denotes	of
T5336	29292-29296	CC	denotes	both
T5337	29297-29311	NNS	denotes	photoreceptors
T5338	29312-29315	CC	denotes	and
T5339	29316-29330	JJ	denotes	postreceptoral
T5340	29331-29338	NNS	denotes	neurons
T5341	29338-29339	.	denotes	.
T5342	29339-29481	sentence	denotes	Anesthetics, particularly ketamine, can cause sustained elevation of glucose in mice, which in turn affects electroretinogram responses [39].
T5343	29340-29351	NNS	denotes	Anesthetics
T5345	29351-29353	,	denotes	,
T5346	29353-29365	RB	denotes	particularly
T5347	29366-29374	NN	denotes	ketamine
T5348	29374-29376	,	denotes	,
T5349	29376-29379	MD	denotes	can
T5344	29380-29385	VB	denotes	cause
T5350	29386-29395	JJ	denotes	sustained
T5351	29396-29405	NN	denotes	elevation
T5352	29406-29408	IN	denotes	of
T5353	29409-29416	NN	denotes	glucose
T5354	29417-29419	IN	denotes	in
T5355	29420-29424	NNS	denotes	mice
T5356	29424-29426	,	denotes	,
T5357	29426-29431	WDT	denotes	which
T5359	29432-29434	IN	denotes	in
T5360	29435-29439	NN	denotes	turn
T5358	29440-29447	VBZ	denotes	affects
T5361	29448-29465	NN	denotes	electroretinogram
T5362	29466-29475	NNS	denotes	responses
T5363	29476-29477	-LRB-	denotes	[
T5364	29477-29479	CD	denotes	39
T5365	29479-29480	-RRB-	denotes	]
T5366	29480-29481	.	denotes	.
T5367	29481-29662	sentence	denotes	Thus, we were concerned that differences in electroretinogram amplitudes between RanBP2+/− and RanBP2+/+ may reflect differences in glucose level changes in response to anesthesia.
T5368	29482-29486	RB	denotes	Thus
T5370	29486-29488	,	denotes	,
T5371	29488-29490	PRP	denotes	we
T5369	29491-29495	VBD	denotes	were
T5372	29496-29505	JJ	denotes	concerned
T5373	29506-29510	IN	denotes	that
T5375	29511-29522	NNS	denotes	differences
T5376	29523-29525	IN	denotes	in
T5377	29526-29543	NN	denotes	electroretinogram
T5378	29544-29554	NNS	denotes	amplitudes
T5379	29555-29562	IN	denotes	between
T5380	29563-29569	NN	denotes	RanBP2
T5381	29569-29570	SYM	denotes	+
T5382	29570-29571	HYPH	denotes	/
T5383	29571-29572	SYM	denotes	−
T5384	29573-29576	CC	denotes	and
T5385	29577-29583	NN	denotes	RanBP2
T5386	29583-29584	SYM	denotes	+
T5387	29584-29585	HYPH	denotes	/
T5388	29585-29586	SYM	denotes	+
T5389	29587-29590	MD	denotes	may
T5374	29591-29598	VB	denotes	reflect
T5390	29599-29610	NNS	denotes	differences
T5391	29611-29613	IN	denotes	in
T5392	29614-29621	NN	denotes	glucose
T5393	29622-29627	NN	denotes	level
T5394	29628-29635	NNS	denotes	changes
T5395	29636-29638	IN	denotes	in
T5396	29639-29647	NN	denotes	response
T5397	29648-29650	IN	denotes	to
T5398	29651-29661	NN	denotes	anesthesia
T5399	29661-29662	.	denotes	.
T5400	29662-29797	sentence	denotes	However, we found no significant differences in glucose levels measured before and every 15 min during 75 min of anesthesia (n = 4–5).
T5401	29663-29670	RB	denotes	However
T5403	29670-29672	,	denotes	,
T5404	29672-29674	PRP	denotes	we
T5402	29675-29680	VBD	denotes	found
T5405	29681-29683	DT	denotes	no
T5407	29684-29695	JJ	denotes	significant
T5406	29696-29707	NNS	denotes	differences
T5408	29708-29710	IN	denotes	in
T5409	29711-29718	NN	denotes	glucose
T5410	29719-29725	NNS	denotes	levels
T5411	29726-29734	VBN	denotes	measured
T5412	29735-29741	IN	denotes	before
T5413	29742-29745	CC	denotes	and
T5414	29746-29751	DT	denotes	every
T5416	29752-29754	CD	denotes	15
T5415	29755-29758	NNS	denotes	min
T5417	29759-29765	IN	denotes	during
T5419	29766-29768	CD	denotes	75
T5418	29769-29772	NNS	denotes	min
T5420	29773-29775	IN	denotes	of
T5421	29776-29786	NN	denotes	anesthesia
T5422	29787-29788	-LRB-	denotes	(
T5424	29788-29789	NN	denotes	n
T5425	29790-29791	SYM	denotes	=
T5426	29792-29793	CD	denotes	4
T5427	29793-29794	SYM	denotes	–
T5423	29794-29795	CD	denotes	5
T5428	29795-29796	-RRB-	denotes	)
T5429	29796-29797	.	denotes	.
T5430	29797-29939	sentence	denotes	Glucose rose at the same rate and reached a maximum of approximately 3.3 times the pre-anesthesia level in both genotypes (unpublished data).
T5431	29798-29805	NN	denotes	Glucose
T5432	29806-29810	VBD	denotes	rose
T5433	29811-29813	IN	denotes	at
T5434	29814-29817	DT	denotes	the
T5436	29818-29822	JJ	denotes	same
T5435	29823-29827	NN	denotes	rate
T5437	29828-29831	CC	denotes	and
T5438	29832-29839	VBD	denotes	reached
T5439	29840-29841	DT	denotes	a
T5440	29842-29849	NN	denotes	maximum
T5441	29850-29852	IN	denotes	of
T5442	29853-29866	RB	denotes	approximately
T5443	29867-29870	CD	denotes	3.3
T5445	29871-29876	NNS	denotes	times
T5446	29877-29880	DT	denotes	the
T5447	29881-29895	JJ	denotes	pre-anesthesia
T5444	29896-29901	NN	denotes	level
T5448	29902-29904	IN	denotes	in
T5449	29905-29909	DT	denotes	both
T5450	29910-29919	NNS	denotes	genotypes
T5451	29920-29921	-LRB-	denotes	(
T5453	29921-29932	JJ	denotes	unpublished
T5452	29933-29937	NNS	denotes	data
T5454	29937-29938	-RRB-	denotes	)
T5455	29938-29939	.	denotes	.
T14065	29950-29968	NNS	denotes	Electroretinograms
T14066	29969-29973	IN	denotes	from
T14067	29974-29975	CD	denotes	6
T14069	29975-29976	HYPH	denotes	-
T14068	29976-29978	NN	denotes	Mo
T14071	29978-29979	HYPH	denotes	-
T14070	29979-29982	JJ	denotes	Old
T14073	29983-29989	NN	denotes	RanBP2
T14074	29989-29990	SYM	denotes	+
T14075	29990-29991	HYPH	denotes	/
T14076	29991-29992	SYM	denotes	−
T14077	29992-29995	CC	denotes	and
T14078	29996-30002	NN	denotes	RanBP2
T14079	30002-30003	SYM	denotes	+
T14080	30003-30004	HYPH	denotes	/
T14081	30004-30005	SYM	denotes	+
T14082	30006-30012	JJ	denotes	Inbred
T14072	30013-30017	NNS	denotes	Mice
T14083	30018-30025	VBG	denotes	Showing
T14084	30026-30039	NN	denotes	Photoreceptor
T14086	30040-30043	CC	denotes	and
T14087	30044-30056	NN	denotes	Postreceptor
T14085	30057-30063	NN	denotes	Neuron
T14089	30064-30084	JJ	denotes	Electrophysiological
T14090	30085-30093	NN	denotes	Response
T14088	30094-30104	NNS	denotes	Phenotypes
T14091	30104-30298	sentence	denotes	(A) Scotopic (dark-adapted) responses from RanBP2+/− mice to light stimuli of increasing intensity, beginning at threshold, have reduced amplitudes compared to those observed in RanBP2+/+ mice.
T14092	30105-30106	-LRB-	denotes	(
T14093	30106-30107	LS	denotes	A
T14095	30107-30108	-RRB-	denotes	)
T14096	30109-30117	JJ	denotes	Scotopic
T14098	30118-30119	-LRB-	denotes	(
T14100	30119-30123	NN	denotes	dark
T14101	30123-30124	HYPH	denotes	-
T14099	30124-30131	VBN	denotes	adapted
T14102	30131-30132	-RRB-	denotes	)
T14097	30133-30142	NNS	denotes	responses
T14103	30143-30147	IN	denotes	from
T14104	30148-30154	NN	denotes	RanBP2
T14106	30154-30155	SYM	denotes	+
T14107	30155-30156	HYPH	denotes	/
T14108	30156-30157	SYM	denotes	−
T14105	30158-30162	NNS	denotes	mice
T14109	30163-30165	IN	denotes	to
T14110	30166-30171	JJ	denotes	light
T14111	30172-30179	NNS	denotes	stimuli
T14112	30180-30182	IN	denotes	of
T14113	30183-30193	VBG	denotes	increasing
T14114	30194-30203	NN	denotes	intensity
T14115	30203-30205	,	denotes	,
T14116	30205-30214	VBG	denotes	beginning
T14117	30215-30217	IN	denotes	at
T14118	30218-30227	NN	denotes	threshold
T14119	30227-30229	,	denotes	,
T14094	30229-30233	VBP	denotes	have
T14120	30234-30241	JJ	denotes	reduced
T14121	30242-30252	NNS	denotes	amplitudes
T14122	30253-30261	VBN	denotes	compared
T14123	30262-30264	IN	denotes	to
T14124	30265-30270	DT	denotes	those
T14125	30271-30279	VBN	denotes	observed
T14126	30280-30282	IN	denotes	in
T14127	30283-30289	NN	denotes	RanBP2
T14129	30289-30290	SYM	denotes	+
T14130	30290-30291	HYPH	denotes	/
T14131	30291-30292	SYM	denotes	+
T14128	30293-30297	NNS	denotes	mice
T14132	30297-30298	.	denotes	.
T14133	30298-30399	sentence	denotes	The three lower intensities represent responses generated in the rod photoreceptor neuronal pathway.
T14134	30299-30302	DT	denotes	The
T14136	30303-30308	CD	denotes	three
T14137	30309-30314	JJR	denotes	lower
T14135	30315-30326	NNS	denotes	intensities
T14138	30327-30336	VBP	denotes	represent
T14139	30337-30346	NNS	denotes	responses
T14140	30347-30356	VBN	denotes	generated
T14141	30357-30359	IN	denotes	in
T14142	30360-30363	DT	denotes	the
T14144	30364-30367	NN	denotes	rod
T14145	30368-30381	NN	denotes	photoreceptor
T14146	30382-30390	JJ	denotes	neuronal
T14143	30391-30398	NN	denotes	pathway
T14147	30398-30399	.	denotes	.
T14148	30399-30493	sentence	denotes	The upper intensities are comprised of responses generated in both the rod and cone pathways.
T14149	30400-30403	DT	denotes	The
T14151	30404-30409	JJ	denotes	upper
T14150	30410-30421	NNS	denotes	intensities
T14153	30422-30425	VBP	denotes	are
T14152	30426-30435	VBN	denotes	comprised
T14154	30436-30438	IN	denotes	of
T14155	30439-30448	NNS	denotes	responses
T14156	30449-30458	VBN	denotes	generated
T14157	30459-30461	IN	denotes	in
T14158	30462-30466	CC	denotes	both
T14160	30467-30470	DT	denotes	the
T14161	30471-30474	NN	denotes	rod
T14162	30475-30478	CC	denotes	and
T14163	30479-30483	NN	denotes	cone
T14159	30484-30492	NNS	denotes	pathways
T14164	30492-30493	.	denotes	.
T14165	30493-30699	sentence	denotes	(B) Photopic (light-adapted, cone photoreceptor pathway) responses of RanBP2+/− mice to increasing light stimulus intensities also exhibited reduced amplitudes compared to those observed in RanBP2+/+ mice.
T14166	30494-30495	-LRB-	denotes	(
T14167	30495-30496	LS	denotes	B
T14169	30496-30497	-RRB-	denotes	)
T14170	30498-30506	JJ	denotes	Photopic
T14172	30507-30508	-LRB-	denotes	(
T14174	30508-30513	NN	denotes	light
T14176	30513-30514	HYPH	denotes	-
T14175	30514-30521	VBN	denotes	adapted
T14177	30521-30523	,	denotes	,
T14178	30523-30527	NN	denotes	cone
T14179	30528-30541	NN	denotes	photoreceptor
T14173	30542-30549	NN	denotes	pathway
T14180	30549-30550	-RRB-	denotes	)
T14171	30551-30560	NNS	denotes	responses
T14181	30561-30563	IN	denotes	of
T14182	30564-30570	NN	denotes	RanBP2
T14184	30570-30571	SYM	denotes	+
T14185	30571-30572	HYPH	denotes	/
T14186	30572-30573	SYM	denotes	−
T14183	30574-30578	NNS	denotes	mice
T14187	30579-30581	IN	denotes	to
T14188	30582-30592	VBG	denotes	increasing
T14190	30593-30598	JJ	denotes	light
T14191	30599-30607	NN	denotes	stimulus
T14189	30608-30619	NNS	denotes	intensities
T14192	30620-30624	RB	denotes	also
T14168	30625-30634	VBD	denotes	exhibited
T14193	30635-30642	VBN	denotes	reduced
T14194	30643-30653	NNS	denotes	amplitudes
T14195	30654-30662	VBN	denotes	compared
T14196	30663-30665	IN	denotes	to
T14197	30666-30671	DT	denotes	those
T14198	30672-30680	VBN	denotes	observed
T14199	30681-30683	IN	denotes	in
T14200	30684-30690	NN	denotes	RanBP2
T14202	30690-30691	SYM	denotes	+
T14203	30691-30692	HYPH	denotes	/
T14204	30692-30693	SYM	denotes	+
T14201	30694-30698	NNS	denotes	mice
T14205	30698-30699	.	denotes	.
T14206	30699-30862	sentence	denotes	(C) Average ± SE (n = 9) scotopic b-wave amplitudes from RanBP2+/− (open circles) and RanBP2+/+ (filled squares) mice representing postreceptoral neuron function.
T14207	30700-30701	-LRB-	denotes	(
T14208	30701-30702	LS	denotes	C
T14210	30702-30703	-RRB-	denotes	)
T14211	30704-30711	NN	denotes	Average
T14213	30712-30713	SYM	denotes	±
T14212	30714-30716	NN	denotes	SE
T14214	30717-30718	-LRB-	denotes	(
T14216	30718-30719	NN	denotes	n
T14217	30720-30721	SYM	denotes	=
T14215	30722-30723	CD	denotes	9
T14218	30723-30724	-RRB-	denotes	)
T14219	30725-30733	JJ	denotes	scotopic
T14220	30734-30735	NN	denotes	b
T14222	30735-30736	HYPH	denotes	-
T14221	30736-30740	NN	denotes	wave
T14209	30741-30751	NNS	denotes	amplitudes
T14223	30752-30756	IN	denotes	from
T14224	30757-30763	NN	denotes	RanBP2
T14226	30763-30764	SYM	denotes	+
T14227	30764-30765	HYPH	denotes	/
T14228	30765-30766	SYM	denotes	−
T14229	30767-30768	-LRB-	denotes	(
T14231	30768-30772	JJ	denotes	open
T14230	30773-30780	NNS	denotes	circles
T14232	30780-30781	-RRB-	denotes	)
T14233	30782-30785	CC	denotes	and
T14234	30786-30792	NN	denotes	RanBP2
T14235	30792-30793	SYM	denotes	+
T14236	30793-30794	HYPH	denotes	/
T14237	30794-30795	SYM	denotes	+
T14238	30796-30797	-LRB-	denotes	(
T14240	30797-30803	VBN	denotes	filled
T14239	30804-30811	NNS	denotes	squares
T14241	30811-30812	-RRB-	denotes	)
T14225	30813-30817	NNS	denotes	mice
T14242	30818-30830	VBG	denotes	representing
T14243	30831-30845	JJ	denotes	postreceptoral
T14244	30846-30852	NN	denotes	neuron
T14245	30853-30861	NN	denotes	function
T14246	30861-30862	.	denotes	.
T14247	30862-30891	sentence	denotes	(Note: log amplitude scale.)
T14248	30863-30864	-LRB-	denotes	(
T14250	30864-30868	NN	denotes	Note
T14251	30868-30870	:	denotes	:
T14252	30870-30873	NN	denotes	log
T14253	30874-30883	NN	denotes	amplitude
T14249	30884-30889	NN	denotes	scale
T14254	30889-30890	.	denotes	.
T14255	30890-30891	-RRB-	denotes	)
T14256	30891-31045	sentence	denotes	(D) Average ± SE (n = 5) scotopic a-wave amplitudes, representing photoreceptor function, for RanBP2+/− and RanBP2+/+ mice in response to bright flashes.
T14257	30892-30893	-LRB-	denotes	(
T14258	30893-30894	LS	denotes	D
T14260	30894-30895	-RRB-	denotes	)
T14261	30896-30903	JJ	denotes	Average
T14263	30904-30905	SYM	denotes	±
T14262	30906-30908	NN	denotes	SE
T14264	30909-30910	-LRB-	denotes	(
T14266	30910-30911	NN	denotes	n
T14267	30912-30913	SYM	denotes	=
T14265	30914-30915	CD	denotes	5
T14268	30915-30916	-RRB-	denotes	)
T14269	30917-30925	JJ	denotes	scotopic
T14270	30926-30927	NN	denotes	a
T14272	30927-30928	HYPH	denotes	-
T14271	30928-30932	NN	denotes	wave
T14259	30933-30943	NNS	denotes	amplitudes
T14273	30943-30945	,	denotes	,
T14274	30945-30957	VBG	denotes	representing
T14275	30958-30971	NN	denotes	photoreceptor
T14276	30972-30980	NN	denotes	function
T14277	30980-30982	,	denotes	,
T14278	30982-30985	IN	denotes	for
T14279	30986-30992	NN	denotes	RanBP2
T14281	30992-30993	SYM	denotes	+
T14282	30993-30994	HYPH	denotes	/
T14283	30994-30995	SYM	denotes	−
T14284	30996-30999	CC	denotes	and
T14285	31000-31006	NN	denotes	RanBP2
T14286	31006-31007	SYM	denotes	+
T14287	31007-31008	HYPH	denotes	/
T14288	31008-31009	SYM	denotes	+
T14280	31010-31014	NNS	denotes	mice
T14289	31015-31017	IN	denotes	in
T14290	31018-31026	NN	denotes	response
T14291	31027-31029	IN	denotes	to
T14292	31030-31036	JJ	denotes	bright
T14293	31037-31044	NNS	denotes	flashes
T14294	31044-31045	.	denotes	.
T14295	31045-31171	sentence	denotes	Amplitudes of responses from RanBP2+/− mice were lower over the entire range of stimulus intensities for both b- and a-waves.
T14296	31046-31056	NNS	denotes	Amplitudes
T14298	31057-31059	IN	denotes	of
T14299	31060-31069	NNS	denotes	responses
T14300	31070-31074	IN	denotes	from
T14301	31075-31081	NN	denotes	RanBP2
T14303	31081-31082	SYM	denotes	+
T14304	31082-31083	HYPH	denotes	/
T14305	31083-31084	SYM	denotes	−
T14302	31085-31089	NNS	denotes	mice
T14297	31090-31094	VBD	denotes	were
T14306	31095-31100	JJR	denotes	lower
T14307	31101-31105	IN	denotes	over
T14308	31106-31109	DT	denotes	the
T14310	31110-31116	JJ	denotes	entire
T14309	31117-31122	NN	denotes	range
T14311	31123-31125	IN	denotes	of
T14312	31126-31134	NN	denotes	stimulus
T14313	31135-31146	NNS	denotes	intensities
T14314	31147-31150	IN	denotes	for
T14315	31151-31155	CC	denotes	both
T14316	31156-31157	NN	denotes	b
T14318	31157-31158	HYPH	denotes	-
T14319	31159-31162	CC	denotes	and
T14320	31163-31164	NN	denotes	a
T14321	31164-31165	HYPH	denotes	-
T14317	31165-31170	NNS	denotes	waves
T14322	31170-31171	.	denotes	.
T14323	31171-31264	sentence	denotes	Asterisks represent significant differences between the groups (Student's t test, p < 0.05).
T14324	31172-31181	NNS	denotes	Asterisks
T14325	31182-31191	VBP	denotes	represent
T14326	31192-31203	JJ	denotes	significant
T14327	31204-31215	NNS	denotes	differences
T14328	31216-31223	IN	denotes	between
T14329	31224-31227	DT	denotes	the
T14330	31228-31234	NNS	denotes	groups
T14331	31235-31236	-LRB-	denotes	(
T14333	31236-31243	NN	denotes	Student
T14334	31243-31245	POS	denotes	's
T14335	31246-31247	NN	denotes	t
T14332	31248-31252	NN	denotes	test
T14336	31252-31254	,	denotes	,
T14337	31254-31255	NN	denotes	p
T14339	31256-31257	SYM	denotes	<
T14338	31258-31262	CD	denotes	0.05
T14340	31262-31263	-RRB-	denotes	)
T14341	31263-31264	.	denotes	.
T14342	31264-31395	sentence	denotes	Statistical significance was found across all intensities for b-wave amplitudes (2-way ANOVA, p < 0.0001), but not for the a-wave.
T14343	31265-31276	JJ	denotes	Statistical
T14344	31277-31289	NN	denotes	significance
T14346	31290-31293	VBD	denotes	was
T14345	31294-31299	VBN	denotes	found
T14347	31300-31306	IN	denotes	across
T14348	31307-31310	DT	denotes	all
T14349	31311-31322	NNS	denotes	intensities
T14350	31323-31326	IN	denotes	for
T14351	31327-31328	NN	denotes	b
T14353	31328-31329	HYPH	denotes	-
T14352	31329-31333	NN	denotes	wave
T14354	31334-31344	NNS	denotes	amplitudes
T14355	31345-31346	-LRB-	denotes	(
T14357	31346-31347	CD	denotes	2
T14359	31347-31348	HYPH	denotes	-
T14358	31348-31351	NN	denotes	way
T14356	31352-31357	NNP	denotes	ANOVA
T14360	31357-31359	,	denotes	,
T14361	31359-31360	NN	denotes	p
T14363	31361-31362	SYM	denotes	<
T14362	31363-31369	CD	denotes	0.0001
T14364	31369-31370	-RRB-	denotes	)
T14365	31370-31372	,	denotes	,
T14366	31372-31375	CC	denotes	but
T14367	31376-31379	RB	denotes	not
T14368	31380-31383	IN	denotes	for
T14369	31384-31387	DT	denotes	the
T14371	31388-31389	NN	denotes	a
T14372	31389-31390	HYPH	denotes	-
T14370	31390-31394	NN	denotes	wave
T14373	31394-31395	.	denotes	.
T6106	31408-31411	PRP$	denotes	Our
T6107	31412-31420	NNS	denotes	findings
T6108	31421-31428	VBP	denotes	support
T6109	31429-31433	IN	denotes	that
T6111	31434-31440	NN	denotes	RanBP2
T6110	31441-31446	VBZ	denotes	plays
T6112	31447-31448	DT	denotes	a
T6114	31449-31460	JJ	denotes	determinant
T6113	31461-31465	NN	denotes	role
T6115	31466-31468	IN	denotes	in
T6116	31469-31479	VBG	denotes	modulating
T6117	31480-31487	NN	denotes	glucose
T6118	31488-31491	CC	denotes	and
T6119	31492-31498	NN	denotes	energy
T6120	31499-31510	NN	denotes	homeostasis
T6121	31510-31511	.	denotes	.
T6122	31511-31619	sentence	denotes	The data support that Cox1 and HKI are novel partners in vivo for the large LD of RanBP2 (Figures 1 and 2).
T6123	31512-31515	DT	denotes	The
T6124	31516-31520	NNS	denotes	data
T6125	31521-31528	VBP	denotes	support
T6126	31529-31533	IN	denotes	that
T6128	31534-31538	NN	denotes	Cox1
T6129	31539-31542	CC	denotes	and
T6130	31543-31546	NN	denotes	HKI
T6127	31547-31550	VBP	denotes	are
T6131	31551-31556	JJ	denotes	novel
T6132	31557-31565	NNS	denotes	partners
T6133	31566-31568	FW	denotes	in
T6134	31569-31573	FW	denotes	vivo
T6135	31574-31577	IN	denotes	for
T6136	31578-31581	DT	denotes	the
T6138	31582-31587	JJ	denotes	large
T6137	31588-31590	NN	denotes	LD
T6139	31591-31593	IN	denotes	of
T6140	31594-31600	NN	denotes	RanBP2
T6141	31601-31602	-LRB-	denotes	(
T6143	31602-31609	NNS	denotes	Figures
T6142	31610-31611	CD	denotes	1
T6144	31612-31615	CC	denotes	and
T6145	31616-31617	CD	denotes	2
T6146	31617-31618	-RRB-	denotes	)
T6147	31618-31619	.	denotes	.
T6148	31619-31744	sentence	denotes	The LD of RanBP2 exhibits chaperone activity toward folding intermediates of Cox11, and possibly, the mature HKI (Figure 1).
T6149	31620-31623	DT	denotes	The
T6150	31624-31626	NN	denotes	LD
T6152	31627-31629	IN	denotes	of
T6153	31630-31636	NN	denotes	RanBP2
T6151	31637-31645	VBZ	denotes	exhibits
T6154	31646-31655	NN	denotes	chaperone
T6155	31656-31664	NN	denotes	activity
T6156	31665-31671	IN	denotes	toward
T6157	31672-31679	NN	denotes	folding
T6158	31680-31693	NNS	denotes	intermediates
T6159	31694-31696	IN	denotes	of
T6160	31697-31702	NN	denotes	Cox11
T6161	31702-31704	,	denotes	,
T6162	31704-31707	CC	denotes	and
T6163	31708-31716	RB	denotes	possibly
T6165	31716-31718	,	denotes	,
T6166	31718-31721	DT	denotes	the
T6167	31722-31728	JJ	denotes	mature
T6164	31729-31732	NN	denotes	HKI
T6168	31733-31734	-LRB-	denotes	(
T6169	31734-31740	NN	denotes	Figure
T6170	31741-31742	CD	denotes	1
T6171	31742-31743	-RRB-	denotes	)
T6172	31743-31744	.	denotes	.
T6173	31744-31946	sentence	denotes	Cox11 inhibits noncompetitively the activity of HKI with ~2–3 molecules of Cox11 (assuming formation of Cox11 dimer) required to inhibit completely the activity of a molecule of HKI (Figure 2A and 2B).
T6174	31745-31750	NN	denotes	Cox11
T6175	31751-31759	VBZ	denotes	inhibits
T6176	31760-31776	RB	denotes	noncompetitively
T6177	31777-31780	DT	denotes	the
T6178	31781-31789	NN	denotes	activity
T6179	31790-31792	IN	denotes	of
T6180	31793-31796	NN	denotes	HKI
T6181	31797-31801	IN	denotes	with
T6183	31802-31803	SYM	denotes	~
T6185	31803-31804	CD	denotes	2
T6186	31804-31805	SYM	denotes	–
T6184	31805-31806	CD	denotes	3
T6187	31807-31816	NNS	denotes	molecules
T6188	31817-31819	IN	denotes	of
T6189	31820-31825	NN	denotes	Cox11
T6190	31826-31827	-LRB-	denotes	(
T6191	31827-31835	VBG	denotes	assuming
T6192	31836-31845	NN	denotes	formation
T6193	31846-31848	IN	denotes	of
T6194	31849-31854	NN	denotes	Cox11
T6195	31855-31860	NN	denotes	dimer
T6196	31860-31861	-RRB-	denotes	)
T6182	31862-31870	VBN	denotes	required
T6197	31871-31873	TO	denotes	to
T6198	31874-31881	VB	denotes	inhibit
T6199	31882-31892	RB	denotes	completely
T6200	31893-31896	DT	denotes	the
T6201	31897-31905	NN	denotes	activity
T6202	31906-31908	IN	denotes	of
T6203	31909-31910	DT	denotes	a
T6204	31911-31919	NN	denotes	molecule
T6205	31920-31922	IN	denotes	of
T6206	31923-31926	NN	denotes	HKI
T6207	31927-31928	-LRB-	denotes	(
T6209	31928-31934	NN	denotes	Figure
T6208	31935-31937	NN	denotes	2A
T6210	31938-31941	CC	denotes	and
T6211	31942-31944	NN	denotes	2B
T6212	31944-31945	-RRB-	denotes	)
T6213	31945-31946	.	denotes	.
T6214	31946-32101	sentence	denotes	Cox11 sequesters HKI by binding to HKI at a site that is distinct from the active site and effectively reduces the availability of [HKI]tot for catalysis.
T6215	31947-31952	NN	denotes	Cox11
T6216	31953-31963	VBZ	denotes	sequesters
T6217	31964-31967	NN	denotes	HKI
T6218	31968-31970	IN	denotes	by
T6219	31971-31978	VBG	denotes	binding
T6220	31979-31981	IN	denotes	to
T6221	31982-31985	NN	denotes	HKI
T6222	31986-31988	IN	denotes	at
T6223	31989-31990	DT	denotes	a
T6224	31991-31995	NN	denotes	site
T6225	31996-32000	WDT	denotes	that
T6226	32001-32003	VBZ	denotes	is
T6227	32004-32012	JJ	denotes	distinct
T6228	32013-32017	IN	denotes	from
T6229	32018-32021	DT	denotes	the
T6231	32022-32028	JJ	denotes	active
T6230	32029-32033	NN	denotes	site
T6232	32034-32037	CC	denotes	and
T6233	32038-32049	RB	denotes	effectively
T6234	32050-32057	VBZ	denotes	reduces
T6235	32058-32061	DT	denotes	the
T6236	32062-32074	NN	denotes	availability
T6237	32075-32077	IN	denotes	of
T6238	32078-32079	-LRB-	denotes	[
T6240	32079-32082	NN	denotes	HKI
T6241	32082-32083	-RRB-	denotes	]
T6239	32083-32086	NN	denotes	tot
T6242	32087-32090	IN	denotes	for
T6243	32091-32100	NN	denotes	catalysis
T6244	32100-32101	.	denotes	.
T6245	32101-32255	sentence	denotes	This is reflected by a reduction of the V max of HKI without significantly affecting the K m of the active site of HKI toward glucose (Figure 2A and 2B).
T6246	32102-32106	DT	denotes	This
T6248	32107-32109	VBZ	denotes	is
T6247	32110-32119	VBN	denotes	reflected
T6249	32120-32122	IN	denotes	by
T6250	32123-32124	DT	denotes	a
T6251	32125-32134	NN	denotes	reduction
T6252	32135-32137	IN	denotes	of
T6253	32138-32141	DT	denotes	the
T6254	32142-32147	NN	denotes	V max
T6255	32148-32150	IN	denotes	of
T6256	32151-32154	NN	denotes	HKI
T6257	32155-32162	IN	denotes	without
T6258	32163-32176	RB	denotes	significantly
T6259	32177-32186	VBG	denotes	affecting
T6260	32187-32190	DT	denotes	the
T6261	32191-32194	NN	denotes	K m
T6262	32195-32197	IN	denotes	of
T6263	32198-32201	DT	denotes	the
T6265	32202-32208	JJ	denotes	active
T6264	32209-32213	NN	denotes	site
T6266	32214-32216	IN	denotes	of
T6267	32217-32220	NN	denotes	HKI
T6268	32221-32227	IN	denotes	toward
T6269	32228-32235	NN	denotes	glucose
T6270	32236-32237	-LRB-	denotes	(
T6272	32237-32243	NN	denotes	Figure
T6271	32244-32246	NN	denotes	2A
T6273	32247-32250	CC	denotes	and
T6274	32251-32253	NN	denotes	2B
T6275	32253-32254	-RRB-	denotes	)
T6276	32254-32255	.	denotes	.
T6277	32255-32419	sentence	denotes	The inhibitory property of Cox11 over HKI is suppressed by RanBP2 (Figure 2C), which by itself has a weak but significant inhibitory activity over HKI (Figure 2D).
T6278	32256-32259	DT	denotes	The
T6280	32260-32270	JJ	denotes	inhibitory
T6279	32271-32279	NN	denotes	property
T6282	32280-32282	IN	denotes	of
T6283	32283-32288	NN	denotes	Cox11
T6284	32289-32293	IN	denotes	over
T6285	32294-32297	NN	denotes	HKI
T6286	32298-32300	VBZ	denotes	is
T6281	32301-32311	VBN	denotes	suppressed
T6287	32312-32314	IN	denotes	by
T6288	32315-32321	NN	denotes	RanBP2
T6289	32322-32323	-LRB-	denotes	(
T6291	32323-32329	NN	denotes	Figure
T6290	32330-32332	NN	denotes	2C
T6292	32332-32333	-RRB-	denotes	)
T6293	32333-32335	,	denotes	,
T6294	32335-32340	WDT	denotes	which
T6296	32341-32343	IN	denotes	by
T6297	32344-32350	PRP	denotes	itself
T6295	32351-32354	VBZ	denotes	has
T6298	32355-32356	DT	denotes	a
T6300	32357-32361	JJ	denotes	weak
T6301	32362-32365	CC	denotes	but
T6302	32366-32377	JJ	denotes	significant
T6303	32378-32388	JJ	denotes	inhibitory
T6299	32389-32397	NN	denotes	activity
T6304	32398-32402	IN	denotes	over
T6305	32403-32406	NN	denotes	HKI
T6306	32407-32408	-LRB-	denotes	(
T6308	32408-32414	NN	denotes	Figure
T6307	32415-32417	NN	denotes	2D
T6309	32417-32418	-RRB-	denotes	)
T6310	32418-32419	.	denotes	.
T6311	32419-32708	sentence	denotes	The sub-stochiometry effect of the LD of RanBP2 over the inhibition of HKI by Cox11 supports that a LD-dependent chaperonin-like mechanism underlies the suppression of Cox11-dependent inhibition of HKI by RanBP2, and that RanBP2 acts as a molecular “buffer” over HK1 and Cox11 activities.
T6312	32420-32423	DT	denotes	The
T6314	32424-32440	JJ	denotes	sub-stochiometry
T6313	32441-32447	NN	denotes	effect
T6316	32448-32450	IN	denotes	of
T6317	32451-32454	DT	denotes	the
T6318	32455-32457	NN	denotes	LD
T6319	32458-32460	IN	denotes	of
T6320	32461-32467	NN	denotes	RanBP2
T6321	32468-32472	IN	denotes	over
T6322	32473-32476	DT	denotes	the
T6323	32477-32487	NN	denotes	inhibition
T6324	32488-32490	IN	denotes	of
T6325	32491-32494	NN	denotes	HKI
T6326	32495-32497	IN	denotes	by
T6327	32498-32503	NN	denotes	Cox11
T6315	32504-32512	VBZ	denotes	supports
T6328	32513-32517	IN	denotes	that
T6330	32518-32519	DT	denotes	a
T6332	32520-32522	NN	denotes	LD
T6334	32522-32523	HYPH	denotes	-
T6333	32523-32532	JJ	denotes	dependent
T6335	32533-32543	NN	denotes	chaperonin
T6337	32543-32544	HYPH	denotes	-
T6336	32544-32548	JJ	denotes	like
T6331	32549-32558	NN	denotes	mechanism
T6329	32559-32568	VBZ	denotes	underlies
T6338	32569-32572	DT	denotes	the
T6339	32573-32584	NN	denotes	suppression
T6340	32585-32587	IN	denotes	of
T6341	32588-32593	JJ	denotes	Cox11
T6343	32593-32594	HYPH	denotes	-
T6342	32594-32603	JJ	denotes	dependent
T6344	32604-32614	NN	denotes	inhibition
T6345	32615-32617	IN	denotes	of
T6346	32618-32621	NN	denotes	HKI
T6347	32622-32624	IN	denotes	by
T6348	32625-32631	NN	denotes	RanBP2
T6349	32631-32633	,	denotes	,
T6350	32633-32636	CC	denotes	and
T6351	32637-32641	IN	denotes	that
T6353	32642-32648	NN	denotes	RanBP2
T6352	32649-32653	VBZ	denotes	acts
T6354	32654-32656	IN	denotes	as
T6355	32657-32658	DT	denotes	a
T6357	32659-32668	JJ	denotes	molecular
T6358	32669-32670	``	denotes	“
T6356	32670-32676	NN	denotes	buffer
T6359	32676-32677	''	denotes	”
T6360	32678-32682	IN	denotes	over
T6361	32683-32686	NN	denotes	HK1
T6363	32687-32690	CC	denotes	and
T6364	32691-32696	NN	denotes	Cox11
T6362	32697-32707	NNS	denotes	activities
T6365	32707-32708	.	denotes	.
T6366	32708-32881	sentence	denotes	The partial loss of the RanBP2 chaperone activity in RanBP2+/− mice also leads to deficits in the sequestration of HKI in the ellipsoid compartment of photosensory neurons.
T6367	32709-32712	DT	denotes	The
T6369	32713-32720	JJ	denotes	partial
T6368	32721-32725	NN	denotes	loss
T6371	32726-32728	IN	denotes	of
T6372	32729-32732	DT	denotes	the
T6374	32733-32739	NN	denotes	RanBP2
T6375	32740-32749	NN	denotes	chaperone
T6373	32750-32758	NN	denotes	activity
T6376	32759-32761	IN	denotes	in
T6377	32762-32768	NN	denotes	RanBP2
T6379	32768-32769	SYM	denotes	+
T6380	32769-32770	HYPH	denotes	/
T6381	32770-32771	SYM	denotes	−
T6378	32772-32776	NNS	denotes	mice
T6382	32777-32781	RB	denotes	also
T6370	32782-32787	VBZ	denotes	leads
T6383	32788-32790	IN	denotes	to
T6384	32791-32799	NNS	denotes	deficits
T6385	32800-32802	IN	denotes	in
T6386	32803-32806	DT	denotes	the
T6387	32807-32820	NN	denotes	sequestration
T6388	32821-32823	IN	denotes	of
T6389	32824-32827	NN	denotes	HKI
T6390	32828-32830	IN	denotes	in
T6391	32831-32834	DT	denotes	the
T6393	32835-32844	JJ	denotes	ellipsoid
T6392	32845-32856	NN	denotes	compartment
T6394	32857-32859	IN	denotes	of
T6395	32860-32872	JJ	denotes	photosensory
T6396	32873-32880	NNS	denotes	neurons
T6397	32880-32881	.	denotes	.
T6398	32881-32984	sentence	denotes	This possibly underlies the mistargeting of HKI to the myoid compartment of these neurons (Figure S2).
T6399	32882-32886	DT	denotes	This
T6401	32887-32895	RB	denotes	possibly
T6400	32896-32905	VBZ	denotes	underlies
T6402	32906-32909	DT	denotes	the
T6403	32910-32922	NN	denotes	mistargeting
T6404	32923-32925	IN	denotes	of
T6405	32926-32929	NN	denotes	HKI
T6406	32930-32932	IN	denotes	to
T6407	32933-32936	DT	denotes	the
T6409	32937-32942	JJ	denotes	myoid
T6408	32943-32954	NN	denotes	compartment
T6410	32955-32957	IN	denotes	of
T6411	32958-32963	DT	denotes	these
T6412	32964-32971	NNS	denotes	neurons
T6413	32972-32973	-LRB-	denotes	(
T6415	32973-32979	NN	denotes	Figure
T6414	32980-32982	NN	denotes	S2
T6416	32982-32983	-RRB-	denotes	)
T6417	32983-32984	.	denotes	.
T6418	32984-33271	sentence	denotes	The data support that the ultimate pathophysiological outcome in HKI, caused by a reduction in RanBP2 levels and its chaperone activity, is the selective degradation of HKI as reflected by the reduced levels of HKI (and ATP) but not of other mitochondrial and NPC components (Figure 5).
T6419	32985-32988	DT	denotes	The
T6420	32989-32993	NNS	denotes	data
T6421	32994-33001	VBP	denotes	support
T6422	33002-33006	IN	denotes	that
T6424	33007-33010	DT	denotes	the
T6426	33011-33019	JJ	denotes	ultimate
T6427	33020-33038	JJ	denotes	pathophysiological
T6425	33039-33046	NN	denotes	outcome
T6428	33047-33049	IN	denotes	in
T6429	33050-33053	NN	denotes	HKI
T6430	33053-33055	,	denotes	,
T6431	33055-33061	VBN	denotes	caused
T6432	33062-33064	IN	denotes	by
T6433	33065-33066	DT	denotes	a
T6434	33067-33076	NN	denotes	reduction
T6435	33077-33079	IN	denotes	in
T6436	33080-33086	NN	denotes	RanBP2
T6437	33087-33093	NNS	denotes	levels
T6438	33094-33097	CC	denotes	and
T6439	33098-33101	PRP$	denotes	its
T6441	33102-33111	NN	denotes	chaperone
T6440	33112-33120	NN	denotes	activity
T6442	33120-33122	,	denotes	,
T6423	33122-33124	VBZ	denotes	is
T6443	33125-33128	DT	denotes	the
T6445	33129-33138	JJ	denotes	selective
T6444	33139-33150	NN	denotes	degradation
T6446	33151-33153	IN	denotes	of
T6447	33154-33157	NN	denotes	HKI
T6448	33158-33160	IN	denotes	as
T6449	33161-33170	VBN	denotes	reflected
T6450	33171-33173	IN	denotes	by
T6451	33174-33177	DT	denotes	the
T6453	33178-33185	VBN	denotes	reduced
T6452	33186-33192	NNS	denotes	levels
T6454	33193-33195	IN	denotes	of
T6455	33196-33199	NN	denotes	HKI
T6456	33200-33201	-LRB-	denotes	(
T6457	33201-33204	CC	denotes	and
T6458	33205-33208	NN	denotes	ATP
T6459	33208-33209	-RRB-	denotes	)
T6460	33210-33213	CC	denotes	but
T6461	33214-33217	RB	denotes	not
T6462	33218-33220	IN	denotes	of
T6463	33221-33226	JJ	denotes	other
T6465	33227-33240	JJ	denotes	mitochondrial
T6466	33241-33244	CC	denotes	and
T6467	33245-33248	NN	denotes	NPC
T6464	33249-33259	NNS	denotes	components
T6468	33260-33261	-LRB-	denotes	(
T6469	33261-33267	NN	denotes	Figure
T6470	33268-33269	CD	denotes	5
T6471	33269-33270	-RRB-	denotes	)
T6472	33270-33271	.	denotes	.
T6473	33271-33582	sentence	denotes	Through this process, it is also possible that deficits in RanBP2 cause a disturbance in the equilibrium between Cox11, HK1, and RanBP2 by leading to an increase of the inhibitory activity of Cox11 over HKI that promotes the uncoupling of the interaction of HKI from RanBP2, ultimately causing HKI degradation.
T6474	33272-33279	IN	denotes	Through
T6476	33280-33284	DT	denotes	this
T6477	33285-33292	NN	denotes	process
T6478	33292-33294	,	denotes	,
T6479	33294-33296	PRP	denotes	it
T6475	33297-33299	VBZ	denotes	is
T6480	33300-33304	RB	denotes	also
T6481	33305-33313	JJ	denotes	possible
T6482	33314-33318	IN	denotes	that
T6484	33319-33327	NNS	denotes	deficits
T6485	33328-33330	IN	denotes	in
T6486	33331-33337	NN	denotes	RanBP2
T6483	33338-33343	VBP	denotes	cause
T6487	33344-33345	DT	denotes	a
T6488	33346-33357	NN	denotes	disturbance
T6489	33358-33360	IN	denotes	in
T6490	33361-33364	DT	denotes	the
T6491	33365-33376	NN	denotes	equilibrium
T6492	33377-33384	IN	denotes	between
T6493	33385-33390	NN	denotes	Cox11
T6494	33390-33392	,	denotes	,
T6495	33392-33395	NN	denotes	HK1
T6496	33395-33397	,	denotes	,
T6497	33397-33400	CC	denotes	and
T6498	33401-33407	NN	denotes	RanBP2
T6499	33408-33410	IN	denotes	by
T6500	33411-33418	VBG	denotes	leading
T6501	33419-33421	IN	denotes	to
T6502	33422-33424	DT	denotes	an
T6503	33425-33433	NN	denotes	increase
T6504	33434-33436	IN	denotes	of
T6505	33437-33440	DT	denotes	the
T6507	33441-33451	JJ	denotes	inhibitory
T6506	33452-33460	NN	denotes	activity
T6508	33461-33463	IN	denotes	of
T6509	33464-33469	NN	denotes	Cox11
T6510	33470-33474	IN	denotes	over
T6511	33475-33478	NN	denotes	HKI
T6512	33479-33483	WDT	denotes	that
T6513	33484-33492	VBZ	denotes	promotes
T6514	33493-33496	DT	denotes	the
T6515	33497-33507	NN	denotes	uncoupling
T6516	33508-33510	IN	denotes	of
T6517	33511-33514	DT	denotes	the
T6518	33515-33526	NN	denotes	interaction
T6519	33527-33529	IN	denotes	of
T6520	33530-33533	NN	denotes	HKI
T6521	33534-33538	IN	denotes	from
T6522	33539-33545	NN	denotes	RanBP2
T6523	33545-33547	,	denotes	,
T6524	33547-33557	RB	denotes	ultimately
T6525	33558-33565	VBG	denotes	causing
T6526	33566-33569	NN	denotes	HKI
T6527	33570-33581	NN	denotes	degradation
T6528	33581-33582	.	denotes	.
T6529	33582-33763	sentence	denotes	Regardless, lower levels of HKI likely contribute to the decreased levels in ATP, slower growth rates, and diminished ability to metabolize glucose of RanBP2+/− mice (Figures 5–7).
T6530	33583-33593	RB	denotes	Regardless
T6532	33593-33595	,	denotes	,
T6533	33595-33600	JJR	denotes	lower
T6534	33601-33607	NNS	denotes	levels
T6535	33608-33610	IN	denotes	of
T6536	33611-33614	NN	denotes	HKI
T6537	33615-33621	RB	denotes	likely
T6531	33622-33632	VBP	denotes	contribute
T6538	33633-33635	IN	denotes	to
T6539	33636-33639	DT	denotes	the
T6541	33640-33649	VBN	denotes	decreased
T6540	33650-33656	NNS	denotes	levels
T6542	33657-33659	IN	denotes	in
T6543	33660-33663	NN	denotes	ATP
T6544	33663-33665	,	denotes	,
T6545	33665-33671	JJR	denotes	slower
T6547	33672-33678	NN	denotes	growth
T6546	33679-33684	NNS	denotes	rates
T6548	33684-33686	,	denotes	,
T6549	33686-33689	CC	denotes	and
T6550	33690-33700	VBN	denotes	diminished
T6551	33701-33708	NN	denotes	ability
T6552	33709-33711	TO	denotes	to
T6553	33712-33722	VB	denotes	metabolize
T6554	33723-33730	NN	denotes	glucose
T6555	33731-33733	IN	denotes	of
T6556	33734-33740	NN	denotes	RanBP2
T6558	33740-33741	SYM	denotes	+
T6559	33741-33742	HYPH	denotes	/
T6560	33742-33743	SYM	denotes	−
T6557	33744-33748	NNS	denotes	mice
T6561	33749-33750	-LRB-	denotes	(
T6562	33750-33757	NNS	denotes	Figures
T6563	33758-33759	CD	denotes	5
T6564	33759-33760	SYM	denotes	–
T6565	33760-33761	CD	denotes	7
T6566	33761-33762	-RRB-	denotes	)
T6567	33762-33763	.	denotes	.
T6568	33763-33914	sentence	denotes	As discussed subsequently, the ATP deficits in the retina likely account for the reduced electrophysiological responses of retinal neurons (Figure 8).
T6569	33764-33766	IN	denotes	As
T6570	33767-33776	VBN	denotes	discussed
T6572	33777-33789	RB	denotes	subsequently
T6573	33789-33791	,	denotes	,
T6574	33791-33794	DT	denotes	the
T6576	33795-33798	NN	denotes	ATP
T6575	33799-33807	NNS	denotes	deficits
T6577	33808-33810	IN	denotes	in
T6578	33811-33814	DT	denotes	the
T6579	33815-33821	NN	denotes	retina
T6580	33822-33828	RB	denotes	likely
T6571	33829-33836	VBP	denotes	account
T6581	33837-33840	IN	denotes	for
T6582	33841-33844	DT	denotes	the
T6584	33845-33852	VBN	denotes	reduced
T6585	33853-33873	JJ	denotes	electrophysiological
T6583	33874-33883	NNS	denotes	responses
T6586	33884-33886	IN	denotes	of
T6587	33887-33894	JJ	denotes	retinal
T6588	33895-33902	NNS	denotes	neurons
T6589	33903-33904	-LRB-	denotes	(
T6590	33904-33910	NN	denotes	Figure
T6591	33911-33912	CD	denotes	8
T6592	33912-33913	-RRB-	denotes	)
T6593	33913-33914	.	denotes	.
T6594	33914-34007	sentence	denotes	Various domains of RanBP2 have been previously implicated with a chaperone role in the cell.
T6595	33915-33922	JJ	denotes	Various
T6596	33923-33930	NNS	denotes	domains
T6598	33931-33933	IN	denotes	of
T6599	33934-33940	NN	denotes	RanBP2
T6600	33941-33945	VBP	denotes	have
T6601	33946-33950	VBN	denotes	been
T6602	33951-33961	RB	denotes	previously
T6597	33962-33972	VBN	denotes	implicated
T6603	33973-33977	IN	denotes	with
T6604	33978-33979	DT	denotes	a
T6606	33980-33989	NN	denotes	chaperone
T6605	33990-33994	NN	denotes	role
T6607	33995-33997	IN	denotes	in
T6608	33998-34001	DT	denotes	the
T6609	34002-34006	NN	denotes	cell
T6610	34006-34007	.	denotes	.
T6611	34007-34302	sentence	denotes	These include the enhancement of the biogenesis of red/green opsin by the combination of the RBD4-CY domains [6,7] and the stabilization by the Ran-binding domains of RanBP2 of the guanosine triphosphate-bound conformational state of RanGTPase and interaction of Ran with importin-β [11,40,41].
T6612	34008-34013	DT	denotes	These
T6613	34014-34021	VBP	denotes	include
T6614	34022-34025	DT	denotes	the
T6615	34026-34037	NN	denotes	enhancement
T6616	34038-34040	IN	denotes	of
T6617	34041-34044	DT	denotes	the
T6618	34045-34055	NN	denotes	biogenesis
T6619	34056-34058	IN	denotes	of
T6620	34059-34062	JJ	denotes	red
T6622	34062-34063	HYPH	denotes	/
T6621	34063-34068	JJ	denotes	green
T6623	34069-34074	NN	denotes	opsin
T6624	34075-34077	IN	denotes	by
T6625	34078-34081	DT	denotes	the
T6626	34082-34093	NN	denotes	combination
T6627	34094-34096	IN	denotes	of
T6628	34097-34100	DT	denotes	the
T6630	34101-34105	NN	denotes	RBD4
T6632	34105-34106	HYPH	denotes	-
T6631	34106-34108	NN	denotes	CY
T6629	34109-34116	NNS	denotes	domains
T6633	34117-34118	-LRB-	denotes	[
T6635	34118-34119	CD	denotes	6
T6636	34119-34120	,	denotes	,
T6634	34120-34121	CD	denotes	7
T6637	34121-34122	-RRB-	denotes	]
T6638	34123-34126	CC	denotes	and
T6639	34127-34130	DT	denotes	the
T6640	34131-34144	NN	denotes	stabilization
T6641	34145-34147	IN	denotes	by
T6642	34148-34151	DT	denotes	the
T6644	34152-34155	NN	denotes	Ran
T6646	34155-34156	HYPH	denotes	-
T6645	34156-34163	VBG	denotes	binding
T6643	34164-34171	NNS	denotes	domains
T6647	34172-34174	IN	denotes	of
T6648	34175-34181	NN	denotes	RanBP2
T6649	34182-34184	IN	denotes	of
T6650	34185-34188	DT	denotes	the
T6652	34189-34198	NN	denotes	guanosine
T6653	34199-34211	NN	denotes	triphosphate
T6655	34211-34212	HYPH	denotes	-
T6654	34212-34217	VBN	denotes	bound
T6656	34218-34232	JJ	denotes	conformational
T6651	34233-34238	NN	denotes	state
T6657	34239-34241	IN	denotes	of
T6658	34242-34251	NN	denotes	RanGTPase
T6659	34252-34255	CC	denotes	and
T6660	34256-34267	NN	denotes	interaction
T6661	34268-34270	IN	denotes	of
T6662	34271-34274	NN	denotes	Ran
T6663	34275-34279	IN	denotes	with
T6664	34280-34288	NN	denotes	importin
T6666	34288-34289	HYPH	denotes	-
T6665	34289-34290	NN	denotes	β
T6667	34291-34292	-LRB-	denotes	[
T6669	34292-34294	CD	denotes	11
T6670	34294-34295	,	denotes	,
T6671	34295-34297	CD	denotes	40
T6672	34297-34298	,	denotes	,
T6668	34298-34300	CD	denotes	41
T6673	34300-34301	-RRB-	denotes	]
T6674	34301-34302	.	denotes	.
T6675	34302-34500	sentence	denotes	The data herein show that the multi-chaperone role of RanBP2 extends also to its LD in light of its ability to associate to distinct folding species of Cox11 and to prevent HKI from being degraded.
T6676	34303-34306	DT	denotes	The
T6677	34307-34311	NNS	denotes	data
T6679	34312-34318	RB	denotes	herein
T6678	34319-34323	VBP	denotes	show
T6680	34324-34328	IN	denotes	that
T6682	34329-34332	DT	denotes	the
T6684	34333-34348	JJ	denotes	multi-chaperone
T6683	34349-34353	NN	denotes	role
T6685	34354-34356	IN	denotes	of
T6686	34357-34363	NN	denotes	RanBP2
T6681	34364-34371	VBZ	denotes	extends
T6687	34372-34376	RB	denotes	also
T6688	34377-34379	IN	denotes	to
T6689	34380-34383	PRP$	denotes	its
T6690	34384-34386	NN	denotes	LD
T6691	34387-34389	IN	denotes	in
T6692	34390-34395	NN	denotes	light
T6693	34396-34398	IN	denotes	of
T6694	34399-34402	PRP$	denotes	its
T6695	34403-34410	NN	denotes	ability
T6696	34411-34413	TO	denotes	to
T6697	34414-34423	VB	denotes	associate
T6698	34424-34426	IN	denotes	to
T6699	34427-34435	JJ	denotes	distinct
T6701	34436-34443	NN	denotes	folding
T6700	34444-34451	NNS	denotes	species
T6702	34452-34454	IN	denotes	of
T6703	34455-34460	NN	denotes	Cox11
T6704	34461-34464	CC	denotes	and
T6705	34465-34467	TO	denotes	to
T6706	34468-34475	VB	denotes	prevent
T6707	34476-34479	NN	denotes	HKI
T6708	34480-34484	IN	denotes	from
T6709	34485-34490	VBG	denotes	being
T6710	34491-34499	VBN	denotes	degraded
T6711	34499-34500	.	denotes	.
T6712	34500-34621	sentence	denotes	This chaperone function is likely to be complemented by other partners of RanBP2 with similar and pleiotropic functions.
T6713	34501-34505	DT	denotes	This
T6715	34506-34515	NN	denotes	chaperone
T6714	34516-34524	NN	denotes	function
T6716	34525-34527	VBZ	denotes	is
T6717	34528-34534	JJ	denotes	likely
T6718	34535-34537	TO	denotes	to
T6720	34538-34540	VB	denotes	be
T6719	34541-34553	VBN	denotes	complemented
T6721	34554-34556	IN	denotes	by
T6722	34557-34562	JJ	denotes	other
T6723	34563-34571	NNS	denotes	partners
T6724	34572-34574	IN	denotes	of
T6725	34575-34581	NN	denotes	RanBP2
T6726	34582-34586	IN	denotes	with
T6727	34587-34594	JJ	denotes	similar
T6729	34595-34598	CC	denotes	and
T6730	34599-34610	JJ	denotes	pleiotropic
T6728	34611-34620	NNS	denotes	functions
T6731	34620-34621	.	denotes	.
T6732	34621-35036	sentence	denotes	For example, the combination of the CLD of RanBP2 with several subunits of the 19S cap of the proteasome [14], and of its neighboring internal repeat, W1W2/IR with the E3-ubiquitin ligase, parkin [20], and the E2 SUMO-1-conjugating protein, Ubc9 [15] may contribute to the down-regulation of HKI by 26S proteasome-mediated proteolysis and modulation of the molecular and subcellular partitioning of these partners.
T6733	34622-34625	IN	denotes	For
T6735	34626-34633	NN	denotes	example
T6736	34633-34635	,	denotes	,
T6737	34635-34638	DT	denotes	the
T6738	34639-34650	NN	denotes	combination
T6739	34651-34653	IN	denotes	of
T6740	34654-34657	DT	denotes	the
T6741	34658-34661	NN	denotes	CLD
T6742	34662-34664	IN	denotes	of
T6743	34665-34671	NN	denotes	RanBP2
T6744	34672-34676	IN	denotes	with
T6745	34677-34684	JJ	denotes	several
T6746	34685-34693	NNS	denotes	subunits
T6747	34694-34696	IN	denotes	of
T6748	34697-34700	DT	denotes	the
T6750	34701-34704	NN	denotes	19S
T6749	34705-34708	NN	denotes	cap
T6751	34709-34711	IN	denotes	of
T6752	34712-34715	DT	denotes	the
T6753	34716-34726	NN	denotes	proteasome
T6754	34727-34728	-LRB-	denotes	[
T6755	34728-34730	CD	denotes	14
T6756	34730-34731	-RRB-	denotes	]
T6757	34731-34733	,	denotes	,
T6758	34733-34736	CC	denotes	and
T6759	34737-34739	IN	denotes	of
T6761	34740-34743	PRP$	denotes	its
T6763	34744-34755	VBG	denotes	neighboring
T6764	34756-34764	JJ	denotes	internal
T6762	34765-34771	NN	denotes	repeat
T6765	34771-34773	,	denotes	,
T6766	34773-34777	NN	denotes	W1W2
T6768	34777-34778	HYPH	denotes	/
T6767	34778-34780	NN	denotes	IR
T6760	34781-34785	IN	denotes	with
T6769	34786-34789	DT	denotes	the
T6771	34790-34792	NN	denotes	E3
T6773	34792-34793	HYPH	denotes	-
T6772	34793-34802	NN	denotes	ubiquitin
T6770	34803-34809	NN	denotes	ligase
T6774	34809-34811	,	denotes	,
T6775	34811-34817	NN	denotes	parkin
T6776	34818-34819	-LRB-	denotes	[
T6777	34819-34821	CD	denotes	20
T6778	34821-34822	-RRB-	denotes	]
T6779	34822-34824	,	denotes	,
T6780	34824-34827	CC	denotes	and
T6781	34828-34831	DT	denotes	the
T6783	34832-34834	NN	denotes	E2
T6784	34835-34839	NN	denotes	SUMO
T6786	34839-34840	HYPH	denotes	-
T6787	34840-34841	CD	denotes	1
T6788	34841-34842	HYPH	denotes	-
T6785	34842-34853	VBG	denotes	conjugating
T6782	34854-34861	NN	denotes	protein
T6789	34861-34863	,	denotes	,
T6790	34863-34867	NN	denotes	Ubc9
T6791	34868-34869	-LRB-	denotes	[
T6792	34869-34871	CD	denotes	15
T6793	34871-34872	-RRB-	denotes	]
T6794	34873-34876	MD	denotes	may
T6734	34877-34887	VB	denotes	contribute
T6795	34888-34890	IN	denotes	to
T6796	34891-34894	DT	denotes	the
T6798	34895-34899	JJ	denotes	down
T6799	34899-34900	HYPH	denotes	-
T6797	34900-34910	NN	denotes	regulation
T6800	34911-34913	IN	denotes	of
T6801	34914-34917	NN	denotes	HKI
T6802	34918-34920	IN	denotes	by
T6803	34921-34924	NN	denotes	26S
T6804	34925-34935	NN	denotes	proteasome
T6806	34935-34936	HYPH	denotes	-
T6805	34936-34944	VBN	denotes	mediated
T6807	34945-34956	NN	denotes	proteolysis
T6808	34957-34960	CC	denotes	and
T6809	34961-34971	NN	denotes	modulation
T6810	34972-34974	IN	denotes	of
T6811	34975-34978	DT	denotes	the
T6813	34979-34988	JJ	denotes	molecular
T6814	34989-34992	CC	denotes	and
T6815	34993-35004	JJ	denotes	subcellular
T6812	35005-35017	NN	denotes	partitioning
T6816	35018-35020	IN	denotes	of
T6817	35021-35026	DT	denotes	these
T6818	35027-35035	NNS	denotes	partners
T6819	35035-35036	.	denotes	.
T6820	35036-35291	sentence	denotes	In this regard, it will be interesting to probe whether parkin also causes deficits in HKI in dopaminergic neurons, since parkin was reported to modulate RanBP2 turnover [20], and parkin loss-of-function also causes energy and growth deficits [26–29,42].
T6821	35037-35039	IN	denotes	In
T6823	35040-35044	DT	denotes	this
T6824	35045-35051	NN	denotes	regard
T6825	35051-35053	,	denotes	,
T6826	35053-35055	PRP	denotes	it
T6827	35056-35060	MD	denotes	will
T6822	35061-35063	VB	denotes	be
T6828	35064-35075	JJ	denotes	interesting
T6829	35076-35078	TO	denotes	to
T6830	35079-35084	VB	denotes	probe
T6831	35085-35092	IN	denotes	whether
T6833	35093-35099	NN	denotes	parkin
T6834	35100-35104	RB	denotes	also
T6832	35105-35111	VBZ	denotes	causes
T6835	35112-35120	NNS	denotes	deficits
T6836	35121-35123	IN	denotes	in
T6837	35124-35127	NN	denotes	HKI
T6838	35128-35130	IN	denotes	in
T6839	35131-35143	JJ	denotes	dopaminergic
T6840	35144-35151	NNS	denotes	neurons
T6841	35151-35153	,	denotes	,
T6842	35153-35158	IN	denotes	since
T6844	35159-35165	NN	denotes	parkin
T6845	35166-35169	VBD	denotes	was
T6843	35170-35178	VBN	denotes	reported
T6846	35179-35181	TO	denotes	to
T6847	35182-35190	VB	denotes	modulate
T6848	35191-35197	NN	denotes	RanBP2
T6849	35198-35206	NN	denotes	turnover
T6850	35207-35208	-LRB-	denotes	[
T6851	35208-35210	CD	denotes	20
T6852	35210-35211	-RRB-	denotes	]
T6853	35211-35213	,	denotes	,
T6854	35213-35216	CC	denotes	and
T6855	35217-35223	NN	denotes	parkin
T6856	35224-35228	NN	denotes	loss
T6858	35228-35229	HYPH	denotes	-
T6859	35229-35231	IN	denotes	of
T6860	35231-35232	HYPH	denotes	-
T6861	35232-35240	NN	denotes	function
T6862	35241-35245	RB	denotes	also
T6857	35246-35252	VBZ	denotes	causes
T6863	35253-35259	NN	denotes	energy
T6865	35260-35263	CC	denotes	and
T6866	35264-35270	NN	denotes	growth
T6864	35271-35279	NNS	denotes	deficits
T6867	35280-35281	-LRB-	denotes	[
T6869	35281-35283	CD	denotes	26
T6870	35283-35284	SYM	denotes	–
T6871	35284-35286	CD	denotes	29
T6872	35286-35287	,	denotes	,
T6868	35287-35289	CD	denotes	42
T6873	35289-35290	-RRB-	denotes	]
T6874	35290-35291	.	denotes	.
T6875	35291-35589	sentence	denotes	These data add a new dimension to the complexity of the regulation of the glycolytic pathway, in particular in the CNS, where glycolysis plays a major role in supplying energy and where the proteasome machinery may play a critical role in modulating components of the energy supply machinery [43].
T6876	35292-35297	DT	denotes	These
T6877	35298-35302	NNS	denotes	data
T6878	35303-35306	VBP	denotes	add
T6879	35307-35308	DT	denotes	a
T6881	35309-35312	JJ	denotes	new
T6880	35313-35322	NN	denotes	dimension
T6882	35323-35325	IN	denotes	to
T6883	35326-35329	DT	denotes	the
T6884	35330-35340	NN	denotes	complexity
T6885	35341-35343	IN	denotes	of
T6886	35344-35347	DT	denotes	the
T6887	35348-35358	NN	denotes	regulation
T6888	35359-35361	IN	denotes	of
T6889	35362-35365	DT	denotes	the
T6891	35366-35376	JJ	denotes	glycolytic
T6890	35377-35384	NN	denotes	pathway
T6892	35384-35386	,	denotes	,
T6893	35386-35388	IN	denotes	in
T6895	35389-35399	JJ	denotes	particular
T6894	35400-35402	IN	denotes	in
T6896	35403-35406	DT	denotes	the
T6897	35407-35410	NN	denotes	CNS
T6898	35410-35412	,	denotes	,
T6899	35412-35417	WRB	denotes	where
T6901	35418-35428	NN	denotes	glycolysis
T6900	35429-35434	VBZ	denotes	plays
T6902	35435-35436	DT	denotes	a
T6904	35437-35442	JJ	denotes	major
T6903	35443-35447	NN	denotes	role
T6905	35448-35450	IN	denotes	in
T6906	35451-35460	VBG	denotes	supplying
T6907	35461-35467	NN	denotes	energy
T6908	35468-35471	CC	denotes	and
T6909	35472-35477	WRB	denotes	where
T6911	35478-35481	DT	denotes	the
T6913	35482-35492	NN	denotes	proteasome
T6912	35493-35502	NN	denotes	machinery
T6914	35503-35506	MD	denotes	may
T6910	35507-35511	VB	denotes	play
T6915	35512-35513	DT	denotes	a
T6917	35514-35522	JJ	denotes	critical
T6916	35523-35527	NN	denotes	role
T6918	35528-35530	IN	denotes	in
T6919	35531-35541	VBG	denotes	modulating
T6920	35542-35552	NNS	denotes	components
T6921	35553-35555	IN	denotes	of
T6922	35556-35559	DT	denotes	the
T6924	35560-35566	NN	denotes	energy
T6925	35567-35573	NN	denotes	supply
T6923	35574-35583	NN	denotes	machinery
T6926	35584-35585	-LRB-	denotes	[
T6927	35585-35587	CD	denotes	43
T6928	35587-35588	-RRB-	denotes	]
T6929	35588-35589	.	denotes	.
T6930	35589-35838	sentence	denotes	This is further evidenced by the presence of genetic modifiers in RanBP2+/−mice on a mixed genetic background that compensates for deficits in RanBP2 and deregulation of its partners in glucose/energy homeostasis as observed in the coisogenic line.
T6931	35590-35594	DT	denotes	This
T6933	35595-35597	VBZ	denotes	is
T6934	35598-35605	RB	denotes	further
T6932	35606-35615	VBN	denotes	evidenced
T6935	35616-35618	IN	denotes	by
T6936	35619-35622	DT	denotes	the
T6937	35623-35631	NN	denotes	presence
T6938	35632-35634	IN	denotes	of
T6939	35635-35642	JJ	denotes	genetic
T6940	35643-35652	NNS	denotes	modifiers
T6941	35653-35655	IN	denotes	in
T6942	35656-35662	NN	denotes	RanBP2
T6944	35662-35663	SYM	denotes	+
T6945	35663-35664	HYPH	denotes	/
T6946	35664-35665	SYM	denotes	−
T6943	35665-35669	NNS	denotes	mice
T6947	35670-35672	IN	denotes	on
T6948	35673-35674	DT	denotes	a
T6950	35675-35680	JJ	denotes	mixed
T6951	35681-35688	JJ	denotes	genetic
T6949	35689-35699	NN	denotes	background
T6952	35700-35704	WDT	denotes	that
T6953	35705-35716	VBZ	denotes	compensates
T6954	35717-35720	IN	denotes	for
T6955	35721-35729	NNS	denotes	deficits
T6956	35730-35732	IN	denotes	in
T6957	35733-35739	NN	denotes	RanBP2
T6958	35740-35743	CC	denotes	and
T6959	35744-35756	NN	denotes	deregulation
T6960	35757-35759	IN	denotes	of
T6961	35760-35763	PRP$	denotes	its
T6962	35764-35772	NNS	denotes	partners
T6963	35773-35775	IN	denotes	in
T6964	35776-35783	NN	denotes	glucose
T6966	35783-35784	HYPH	denotes	/
T6965	35784-35790	NN	denotes	energy
T6967	35791-35802	NN	denotes	homeostasis
T6968	35803-35805	IN	denotes	as
T6969	35806-35814	VBN	denotes	observed
T6970	35815-35817	IN	denotes	in
T6971	35818-35821	DT	denotes	the
T6973	35822-35832	JJ	denotes	coisogenic
T6972	35833-35837	NN	denotes	line
T6974	35837-35838	.	denotes	.
T6975	35838-36045	sentence	denotes	The presence of such compensatory mechanisms is also supported by the identification of a quantitative trait locus, which encompasses RanBP2, and modifies the expression of diabetes-related phenotypes [44].
T6976	35839-35842	DT	denotes	The
T6977	35843-35851	NN	denotes	presence
T6979	35852-35854	IN	denotes	of
T6980	35855-35859	JJ	denotes	such
T6982	35860-35872	JJ	denotes	compensatory
T6981	35873-35883	NNS	denotes	mechanisms
T6983	35884-35886	VBZ	denotes	is
T6984	35887-35891	RB	denotes	also
T6978	35892-35901	VBN	denotes	supported
T6985	35902-35904	IN	denotes	by
T6986	35905-35908	DT	denotes	the
T6987	35909-35923	NN	denotes	identification
T6988	35924-35926	IN	denotes	of
T6989	35927-35928	DT	denotes	a
T6991	35929-35941	JJ	denotes	quantitative
T6992	35942-35947	NN	denotes	trait
T6990	35948-35953	NN	denotes	locus
T6993	35953-35955	,	denotes	,
T6994	35955-35960	WDT	denotes	which
T6995	35961-35972	VBZ	denotes	encompasses
T6996	35973-35979	NN	denotes	RanBP2
T6997	35979-35981	,	denotes	,
T6998	35981-35984	CC	denotes	and
T6999	35985-35993	VBZ	denotes	modifies
T7000	35994-35997	DT	denotes	the
T7001	35998-36008	NN	denotes	expression
T7002	36009-36011	IN	denotes	of
T7003	36012-36020	NN	denotes	diabetes
T7005	36020-36021	HYPH	denotes	-
T7004	36021-36028	VBN	denotes	related
T7006	36029-36039	NNS	denotes	phenotypes
T7007	36040-36041	-LRB-	denotes	[
T7008	36041-36043	CD	denotes	44
T7009	36043-36044	-RRB-	denotes	]
T7010	36044-36045	.	denotes	.
T7011	36045-36153	sentence	denotes	The data herein show tissues with a decrease of RanBP2 and HKI levels mirror a reduction in the ATP levels.
T7012	36046-36049	DT	denotes	The
T7013	36050-36054	NNS	denotes	data
T7015	36055-36061	RB	denotes	herein
T7014	36062-36066	VBP	denotes	show
T7016	36067-36074	NNS	denotes	tissues
T7017	36075-36079	IN	denotes	with
T7018	36080-36081	DT	denotes	a
T7019	36082-36090	NN	denotes	decrease
T7020	36091-36093	IN	denotes	of
T7021	36094-36100	NN	denotes	RanBP2
T7022	36101-36104	CC	denotes	and
T7023	36105-36108	NN	denotes	HKI
T7024	36109-36115	NNS	denotes	levels
T7025	36116-36122	VBP	denotes	mirror
T7026	36123-36124	DT	denotes	a
T7027	36125-36134	NN	denotes	reduction
T7028	36135-36137	IN	denotes	in
T7029	36138-36141	DT	denotes	the
T7031	36142-36145	NN	denotes	ATP
T7030	36146-36152	NNS	denotes	levels
T7032	36152-36153	.	denotes	.
T7033	36153-36504	sentence	denotes	Since the constitutive Na+/K+ ATPase pump and the ATP-dependent conversion of glutamine to glutamate (both fundamental to maintain the electrical activity in the CNS) consumes the vast majority of the energy produced by the CNS [45–47], this likely underlies the suppression of the electrophysiological output responses of retinal neurons (Figure 8).
T7034	36154-36159	IN	denotes	Since
T7036	36160-36163	DT	denotes	the
T7038	36164-36176	JJ	denotes	constitutive
T7039	36177-36180	NN	denotes	Na+
T7041	36180-36181	HYPH	denotes	/
T7040	36181-36183	NN	denotes	K+
T7042	36184-36190	NN	denotes	ATPase
T7037	36191-36195	NN	denotes	pump
T7043	36196-36199	CC	denotes	and
T7044	36200-36203	DT	denotes	the
T7046	36204-36207	NN	denotes	ATP
T7048	36207-36208	HYPH	denotes	-
T7047	36208-36217	JJ	denotes	dependent
T7045	36218-36228	NN	denotes	conversion
T7049	36229-36231	IN	denotes	of
T7050	36232-36241	NN	denotes	glutamine
T7051	36242-36244	IN	denotes	to
T7052	36245-36254	NN	denotes	glutamate
T7053	36255-36256	-LRB-	denotes	(
T7054	36256-36260	DT	denotes	both
T7055	36261-36272	JJ	denotes	fundamental
T7056	36273-36275	TO	denotes	to
T7057	36276-36284	VB	denotes	maintain
T7058	36285-36288	DT	denotes	the
T7060	36289-36299	JJ	denotes	electrical
T7059	36300-36308	NN	denotes	activity
T7061	36309-36311	IN	denotes	in
T7062	36312-36315	DT	denotes	the
T7063	36316-36319	NN	denotes	CNS
T7064	36319-36320	-RRB-	denotes	)
T7035	36321-36329	VBZ	denotes	consumes
T7066	36330-36333	DT	denotes	the
T7068	36334-36338	JJ	denotes	vast
T7067	36339-36347	NN	denotes	majority
T7069	36348-36350	IN	denotes	of
T7070	36351-36354	DT	denotes	the
T7071	36355-36361	NN	denotes	energy
T7072	36362-36370	VBN	denotes	produced
T7073	36371-36373	IN	denotes	by
T7074	36374-36377	DT	denotes	the
T7075	36378-36381	NN	denotes	CNS
T7076	36382-36383	-LRB-	denotes	[
T7077	36383-36385	CD	denotes	45
T7078	36385-36386	SYM	denotes	–
T7079	36386-36388	CD	denotes	47
T7080	36388-36389	-RRB-	denotes	]
T7081	36389-36391	,	denotes	,
T7082	36391-36395	DT	denotes	this
T7083	36396-36402	RB	denotes	likely
T7065	36403-36412	VBZ	denotes	underlies
T7084	36413-36416	DT	denotes	the
T7085	36417-36428	NN	denotes	suppression
T7086	36429-36431	IN	denotes	of
T7087	36432-36435	DT	denotes	the
T7089	36436-36456	JJ	denotes	electrophysiological
T7090	36457-36463	NN	denotes	output
T7088	36464-36473	NNS	denotes	responses
T7091	36474-36476	IN	denotes	of
T7092	36477-36484	JJ	denotes	retinal
T7093	36485-36492	NNS	denotes	neurons
T7094	36493-36494	-LRB-	denotes	(
T7095	36494-36500	NN	denotes	Figure
T7096	36501-36502	CD	denotes	8
T7097	36502-36503	-RRB-	denotes	)
T7098	36503-36504	.	denotes	.
T7099	36504-36697	sentence	denotes	Moreover, deficits in HKI may lead to intracellular hyperglycemia in the CNS, promote sorbitol-induced osmotic stress, and compromise further the ATPase-dependent Na+/K+ pump activity [48–50].
T7100	36505-36513	RB	denotes	Moreover
T7102	36513-36515	,	denotes	,
T7103	36515-36523	NNS	denotes	deficits
T7104	36524-36526	IN	denotes	in
T7105	36527-36530	NN	denotes	HKI
T7106	36531-36534	MD	denotes	may
T7101	36535-36539	VB	denotes	lead
T7107	36540-36542	IN	denotes	to
T7108	36543-36556	JJ	denotes	intracellular
T7109	36557-36570	NN	denotes	hyperglycemia
T7110	36571-36573	IN	denotes	in
T7111	36574-36577	DT	denotes	the
T7112	36578-36581	NN	denotes	CNS
T7113	36581-36583	,	denotes	,
T7114	36583-36590	VB	denotes	promote
T7115	36591-36599	NN	denotes	sorbitol
T7117	36599-36600	HYPH	denotes	-
T7116	36600-36607	VBN	denotes	induced
T7119	36608-36615	JJ	denotes	osmotic
T7118	36616-36622	NN	denotes	stress
T7120	36622-36624	,	denotes	,
T7121	36624-36627	CC	denotes	and
T7122	36628-36638	VB	denotes	compromise
T7123	36639-36646	RB	denotes	further
T7124	36647-36650	DT	denotes	the
T7126	36651-36657	NN	denotes	ATPase
T7128	36657-36658	HYPH	denotes	-
T7127	36658-36667	JJ	denotes	dependent
T7129	36668-36671	NN	denotes	Na+
T7131	36671-36672	HYPH	denotes	/
T7130	36672-36674	NN	denotes	K+
T7132	36675-36679	NN	denotes	pump
T7125	36680-36688	NN	denotes	activity
T7133	36689-36690	-LRB-	denotes	[
T7134	36690-36692	CD	denotes	48
T7135	36692-36693	SYM	denotes	–
T7136	36693-36695	CD	denotes	50
T7137	36695-36696	-RRB-	denotes	]
T7138	36696-36697	.	denotes	.
T7139	36697-36801	sentence	denotes	This may be exacerbated by a decrease of ATP, since the activity of HKI is also stimulated by ATP [51].
T7140	36698-36702	DT	denotes	This
T7142	36703-36706	MD	denotes	may
T7143	36707-36709	VB	denotes	be
T7141	36710-36721	VBN	denotes	exacerbated
T7144	36722-36724	IN	denotes	by
T7145	36725-36726	DT	denotes	a
T7146	36727-36735	NN	denotes	decrease
T7147	36736-36738	IN	denotes	of
T7148	36739-36742	NN	denotes	ATP
T7149	36742-36744	,	denotes	,
T7150	36744-36749	IN	denotes	since
T7152	36750-36753	DT	denotes	the
T7153	36754-36762	NN	denotes	activity
T7154	36763-36765	IN	denotes	of
T7155	36766-36769	NN	denotes	HKI
T7156	36770-36772	VBZ	denotes	is
T7157	36773-36777	RB	denotes	also
T7151	36778-36788	VBN	denotes	stimulated
T7158	36789-36791	IN	denotes	by
T7159	36792-36795	NN	denotes	ATP
T7160	36796-36797	-LRB-	denotes	[
T7161	36797-36799	CD	denotes	51
T7162	36799-36800	-RRB-	denotes	]
T7163	36800-36801	.	denotes	.
T7164	36801-36982	sentence	denotes	The cumulative effects of a reduction in ATP and intracellular hyperglycemia are known to act synergistically and modulate the electrophysiological properties of neuronal activity.
T7165	36802-36805	DT	denotes	The
T7167	36806-36816	JJ	denotes	cumulative
T7166	36817-36824	NNS	denotes	effects
T7169	36825-36827	IN	denotes	of
T7170	36828-36829	DT	denotes	a
T7171	36830-36839	NN	denotes	reduction
T7172	36840-36842	IN	denotes	in
T7173	36843-36846	NN	denotes	ATP
T7174	36847-36850	CC	denotes	and
T7175	36851-36864	JJ	denotes	intracellular
T7176	36865-36878	NN	denotes	hyperglycemia
T7177	36879-36882	VBP	denotes	are
T7168	36883-36888	VBN	denotes	known
T7178	36889-36891	TO	denotes	to
T7179	36892-36895	VB	denotes	act
T7180	36896-36911	RB	denotes	synergistically
T7181	36912-36915	CC	denotes	and
T7182	36916-36924	VB	denotes	modulate
T7183	36925-36928	DT	denotes	the
T7185	36929-36949	JJ	denotes	electrophysiological
T7184	36950-36960	NNS	denotes	properties
T7186	36961-36963	IN	denotes	of
T7187	36964-36972	JJ	denotes	neuronal
T7188	36973-36981	NN	denotes	activity
T7189	36981-36982	.	denotes	.
T7190	36982-37076	sentence	denotes	Figure 9 integrates in a model these variables and implications of the data presented herein.
T7191	36983-36989	NN	denotes	Figure
T7193	36990-36991	CD	denotes	9
T7192	36992-37002	VBZ	denotes	integrates
T7194	37003-37005	IN	denotes	in
T7195	37006-37007	DT	denotes	a
T7196	37008-37013	NN	denotes	model
T7197	37014-37019	DT	denotes	these
T7198	37020-37029	NNS	denotes	variables
T7199	37030-37033	CC	denotes	and
T7200	37034-37046	NNS	denotes	implications
T7201	37047-37049	IN	denotes	of
T7202	37050-37053	DT	denotes	the
T7203	37054-37058	NNS	denotes	data
T7204	37059-37068	VBN	denotes	presented
T7205	37069-37075	RB	denotes	herein
T7206	37075-37076	.	denotes	.
T7207	37076-37239	sentence	denotes	Still, other bona fide RanBP2 partners previously identified and described in the Introduction also become strong candidates to play a role in energy homeostasis.
T7208	37077-37082	RB	denotes	Still
T7210	37082-37084	,	denotes	,
T7211	37084-37089	JJ	denotes	other
T7213	37090-37094	FW	denotes	bona
T7214	37095-37099	FW	denotes	fide
T7215	37100-37106	NN	denotes	RanBP2
T7212	37107-37115	NNS	denotes	partners
T7216	37116-37126	RB	denotes	previously
T7217	37127-37137	VBN	denotes	identified
T7218	37138-37141	CC	denotes	and
T7219	37142-37151	VBN	denotes	described
T7220	37152-37154	IN	denotes	in
T7221	37155-37158	DT	denotes	the
T7222	37159-37171	NN	denotes	Introduction
T7223	37172-37176	RB	denotes	also
T7209	37177-37183	VBP	denotes	become
T7224	37184-37190	JJ	denotes	strong
T7225	37191-37201	NNS	denotes	candidates
T7226	37202-37204	TO	denotes	to
T7227	37205-37209	VB	denotes	play
T7228	37210-37211	DT	denotes	a
T7229	37212-37216	NN	denotes	role
T7230	37217-37219	IN	denotes	in
T7231	37220-37226	NN	denotes	energy
T7232	37227-37238	NN	denotes	homeostasis
T7233	37238-37239	.	denotes	.
T7234	37239-37680	sentence	denotes	For example, the unfolding and chaperone activity of components of the 19S cap of the proteasome may be modulated by the CLD of RanBP2 [14] and contribute to the selective HKI (and other substrates) degradation [43], while association of nuclear import receptor, importin-β with the Ran-binding domains of RanBP2 [10,11], may mediate the nuclear translocation of multifunctional substrates, such as glyceraldehyde-3-phosphate dehydrogenase.
T7235	37240-37243	IN	denotes	For
T7237	37244-37251	NN	denotes	example
T7238	37251-37253	,	denotes	,
T7239	37253-37256	DT	denotes	the
T7241	37257-37266	JJ	denotes	unfolding
T7243	37267-37270	CC	denotes	and
T7242	37271-37280	NN	denotes	chaperone
T7240	37281-37289	NN	denotes	activity
T7244	37290-37292	IN	denotes	of
T7245	37293-37303	NNS	denotes	components
T7246	37304-37306	IN	denotes	of
T7247	37307-37310	DT	denotes	the
T7249	37311-37314	NN	denotes	19S
T7248	37315-37318	NN	denotes	cap
T7250	37319-37321	IN	denotes	of
T7251	37322-37325	DT	denotes	the
T7252	37326-37336	NN	denotes	proteasome
T7253	37337-37340	MD	denotes	may
T7254	37341-37343	VB	denotes	be
T7236	37344-37353	VBN	denotes	modulated
T7255	37354-37356	IN	denotes	by
T7256	37357-37360	DT	denotes	the
T7257	37361-37364	NN	denotes	CLD
T7258	37365-37367	IN	denotes	of
T7259	37368-37374	NN	denotes	RanBP2
T7260	37375-37376	-LRB-	denotes	[
T7261	37376-37378	CD	denotes	14
T7262	37378-37379	-RRB-	denotes	]
T7263	37380-37383	CC	denotes	and
T7264	37384-37394	VB	denotes	contribute
T7265	37395-37397	IN	denotes	to
T7266	37398-37401	DT	denotes	the
T7268	37402-37411	JJ	denotes	selective
T7269	37412-37415	NN	denotes	HKI
T7270	37416-37417	-LRB-	denotes	(
T7271	37417-37420	CC	denotes	and
T7272	37421-37426	JJ	denotes	other
T7273	37427-37437	NNS	denotes	substrates
T7274	37437-37438	-RRB-	denotes	)
T7267	37439-37450	NN	denotes	degradation
T7275	37451-37452	-LRB-	denotes	[
T7276	37452-37454	CD	denotes	43
T7277	37454-37455	-RRB-	denotes	]
T7278	37455-37457	,	denotes	,
T7279	37457-37462	IN	denotes	while
T7281	37463-37474	NN	denotes	association
T7282	37475-37477	IN	denotes	of
T7283	37478-37485	JJ	denotes	nuclear
T7285	37486-37492	NN	denotes	import
T7284	37493-37501	NN	denotes	receptor
T7286	37501-37503	,	denotes	,
T7287	37503-37511	NN	denotes	importin
T7289	37511-37512	HYPH	denotes	-
T7288	37512-37513	NN	denotes	β
T7290	37514-37518	IN	denotes	with
T7291	37519-37522	DT	denotes	the
T7293	37523-37526	NN	denotes	Ran
T7295	37526-37527	HYPH	denotes	-
T7294	37527-37534	VBG	denotes	binding
T7292	37535-37542	NNS	denotes	domains
T7296	37543-37545	IN	denotes	of
T7297	37546-37552	NN	denotes	RanBP2
T7298	37553-37554	-LRB-	denotes	[
T7300	37554-37556	CD	denotes	10
T7301	37556-37557	,	denotes	,
T7299	37557-37559	CD	denotes	11
T7302	37559-37560	-RRB-	denotes	]
T7303	37560-37562	,	denotes	,
T7304	37562-37565	MD	denotes	may
T7280	37566-37573	VB	denotes	mediate
T7305	37574-37577	DT	denotes	the
T7307	37578-37585	JJ	denotes	nuclear
T7306	37586-37599	NN	denotes	translocation
T7308	37600-37602	IN	denotes	of
T7309	37603-37618	JJ	denotes	multifunctional
T7310	37619-37629	NNS	denotes	substrates
T7311	37629-37631	,	denotes	,
T7312	37631-37635	JJ	denotes	such
T7313	37636-37638	IN	denotes	as
T7314	37639-37653	NN	denotes	glyceraldehyde
T7316	37653-37654	HYPH	denotes	-
T7317	37654-37655	CD	denotes	3
T7318	37655-37656	HYPH	denotes	-
T7315	37656-37665	NN	denotes	phosphate
T7319	37666-37679	NN	denotes	dehydrogenase
T7320	37679-37680	.	denotes	.
T7321	37680-37856	sentence	denotes	This trafficking process is selectively inhibited by the neuroprotective drug, R-(-)-deprenyl and derivates thereof, and are often employed to treat Parkinson disease [52–57].
T7322	37681-37685	DT	denotes	This
T7324	37686-37697	NN	denotes	trafficking
T7323	37698-37705	NN	denotes	process
T7326	37706-37708	VBZ	denotes	is
T7327	37709-37720	RB	denotes	selectively
T7325	37721-37730	VBN	denotes	inhibited
T7328	37731-37733	IN	denotes	by
T7329	37734-37737	DT	denotes	the
T7331	37738-37753	JJ	denotes	neuroprotective
T7330	37754-37758	NN	denotes	drug
T7332	37758-37760	,	denotes	,
T7333	37760-37761	NN	denotes	R
T7335	37761-37762	HYPH	denotes	-
T7336	37762-37763	-LRB-	denotes	(
T7337	37763-37764	SYM	denotes	-
T7338	37764-37765	-RRB-	denotes	)
T7339	37765-37766	HYPH	denotes	-
T7334	37766-37774	NN	denotes	deprenyl
T7340	37775-37778	CC	denotes	and
T7341	37779-37788	VBZ	denotes	derivates
T7342	37789-37796	RB	denotes	thereof
T7343	37796-37798	,	denotes	,
T7344	37798-37801	CC	denotes	and
T7345	37802-37805	VBP	denotes	are
T7347	37806-37811	RB	denotes	often
T7346	37812-37820	VBN	denotes	employed
T7348	37821-37823	TO	denotes	to
T7349	37824-37829	VB	denotes	treat
T7350	37830-37839	NNP	denotes	Parkinson
T7351	37840-37847	NN	denotes	disease
T7352	37848-37849	-LRB-	denotes	[
T7353	37849-37851	CD	denotes	52
T7354	37851-37852	SYM	denotes	–
T7355	37852-37854	CD	denotes	57
T7356	37854-37855	-RRB-	denotes	]
T7357	37855-37856	.	denotes	.
T7358	37856-38200	sentence	denotes	Hence, RanBP2 and its partners emerge as key players and target genes in mediating neuropathophysiological mechanisms implicated in various genetic and environmental lesions to the CNS, as in patients with Parkinson, diabetes with insulin-resistance, and other neuropathies and neurodegenerative diseases, often linked to aging manifestations.
T7359	37857-37862	RB	denotes	Hence
T7361	37862-37864	,	denotes	,
T7362	37864-37870	NN	denotes	RanBP2
T7363	37871-37874	CC	denotes	and
T7364	37875-37878	PRP$	denotes	its
T7365	37879-37887	NNS	denotes	partners
T7360	37888-37894	VBP	denotes	emerge
T7366	37895-37897	IN	denotes	as
T7367	37898-37901	JJ	denotes	key
T7368	37902-37909	NNS	denotes	players
T7369	37910-37913	CC	denotes	and
T7370	37914-37920	NN	denotes	target
T7371	37921-37926	NNS	denotes	genes
T7372	37927-37929	IN	denotes	in
T7373	37930-37939	VBG	denotes	mediating
T7374	37940-37963	JJ	denotes	neuropathophysiological
T7375	37964-37974	NNS	denotes	mechanisms
T7376	37975-37985	VBN	denotes	implicated
T7377	37986-37988	IN	denotes	in
T7378	37989-37996	JJ	denotes	various
T7380	37997-38004	JJ	denotes	genetic
T7381	38005-38008	CC	denotes	and
T7382	38009-38022	JJ	denotes	environmental
T7379	38023-38030	NNS	denotes	lesions
T7383	38031-38033	IN	denotes	to
T7384	38034-38037	DT	denotes	the
T7385	38038-38041	NN	denotes	CNS
T7386	38041-38043	,	denotes	,
T7387	38043-38045	IN	denotes	as
T7388	38046-38048	IN	denotes	in
T7389	38049-38057	NNS	denotes	patients
T7390	38058-38062	IN	denotes	with
T7391	38063-38072	NNP	denotes	Parkinson
T7392	38072-38074	,	denotes	,
T7393	38074-38082	NN	denotes	diabetes
T7394	38083-38087	IN	denotes	with
T7395	38088-38095	NN	denotes	insulin
T7397	38095-38096	HYPH	denotes	-
T7396	38096-38106	NN	denotes	resistance
T7398	38106-38108	,	denotes	,
T7399	38108-38111	CC	denotes	and
T7400	38112-38117	JJ	denotes	other
T7401	38118-38130	NNS	denotes	neuropathies
T7402	38131-38134	CC	denotes	and
T7403	38135-38152	JJ	denotes	neurodegenerative
T7404	38153-38161	NNS	denotes	diseases
T7405	38161-38163	,	denotes	,
T7406	38163-38168	RB	denotes	often
T7407	38169-38175	VBN	denotes	linked
T7408	38176-38178	IN	denotes	to
T7409	38179-38184	NN	denotes	aging
T7410	38185-38199	NNS	denotes	manifestations
T7411	38199-38200	.	denotes	.
T7412	38200-38434	sentence	denotes	To this effect, the RanBP2 mouse model will serve as a unique genetic tool to probe selective, multiple and novel pathways, which may have not been anticipated to be linked to metabolic processes and allied pathophysiological states.
T7413	38201-38203	IN	denotes	To
T7415	38204-38208	DT	denotes	this
T7416	38209-38215	NN	denotes	effect
T7417	38215-38217	,	denotes	,
T7418	38217-38220	DT	denotes	the
T7420	38221-38227	NN	denotes	RanBP2
T7421	38228-38233	NN	denotes	mouse
T7419	38234-38239	NN	denotes	model
T7422	38240-38244	MD	denotes	will
T7414	38245-38250	VB	denotes	serve
T7423	38251-38253	IN	denotes	as
T7424	38254-38255	DT	denotes	a
T7426	38256-38262	JJ	denotes	unique
T7427	38263-38270	JJ	denotes	genetic
T7425	38271-38275	NN	denotes	tool
T7428	38276-38278	TO	denotes	to
T7429	38279-38284	VB	denotes	probe
T7430	38285-38294	JJ	denotes	selective
T7432	38294-38296	,	denotes	,
T7433	38296-38304	JJ	denotes	multiple
T7434	38305-38308	CC	denotes	and
T7435	38309-38314	JJ	denotes	novel
T7431	38315-38323	NNS	denotes	pathways
T7436	38323-38325	,	denotes	,
T7437	38325-38330	WDT	denotes	which
T7439	38331-38334	MD	denotes	may
T7440	38335-38339	VB	denotes	have
T7441	38340-38343	RB	denotes	not
T7442	38344-38348	VBN	denotes	been
T7438	38349-38360	VBN	denotes	anticipated
T7443	38361-38363	TO	denotes	to
T7445	38364-38366	VB	denotes	be
T7444	38367-38373	VBN	denotes	linked
T7446	38374-38376	IN	denotes	to
T7447	38377-38386	JJ	denotes	metabolic
T7448	38387-38396	NNS	denotes	processes
T7449	38397-38400	CC	denotes	and
T7450	38401-38407	VBN	denotes	allied
T7452	38408-38426	JJ	denotes	pathophysiological
T7451	38427-38433	NNS	denotes	states
T7453	38433-38434	.	denotes	.
T7454	38434-39930	sentence	denotes	Figure 9 Model Depicting a Role of RanBP2 and Some of Its Partners in Metabolic and Neuronal Function RanBP2 interacts with Cox11 and HKI and the triad is in equilibrium under normal physiological conditions. RanBP2 prevents the inhibition of HKI by Cox11 and its degradation. The ultimate effect of RanBP2 on its partners is the stimulation of the glycolytic pathway and production of ATP. The glycolytic pathway is critical to fuel the constitutive Na+/K+-ATPase pump to maintain the dark current between the inner and outer segment compartments of photosensory neurons. A deficit (haploinsufficiency) in RanBP2 disturbs the equilibrium between RanBP2, HKI, and Cox11. This pathophysiological event promotes the destabilization and degradation of HKI and a decrease in ATP production required to maintain the depolarization state neurons, and, hence, a reduction in the response of receptoral and postreceptoral neurons. A reduction in ATP levels also negatively modulates HKI activity/level. Decreased levels of HKI promote intracellular hyperglycemia and activate stress kinases, which modulate negatively the Na+/K+-ATPase pump by phosphorylation. Pathophysiological pathways promoted by RanBP2 haploinsufficiency are represented by dash lines. RIS, rod inner segment; ROS, rod outer segment. Finally, RanBP2 appears to join other nucleoporins, such as Nup96 [58], in novel physiological functions that are vital for complex organisms and that were not anticipated from cell-based studies.
T7455	38435-39741	RB	denotes	Figure 9 Model Depicting a Role of RanBP2 and Some of Its Partners in Metabolic and Neuronal Function RanBP2 interacts with Cox11 and HKI and the triad is in equilibrium under normal physiological conditions. RanBP2 prevents the inhibition of HKI by Cox11 and its degradation. The ultimate effect of RanBP2 on its partners is the stimulation of the glycolytic pathway and production of ATP. The glycolytic pathway is critical to fuel the constitutive Na+/K+-ATPase pump to maintain the dark current between the inner and outer segment compartments of photosensory neurons. A deficit (haploinsufficiency) in RanBP2 disturbs the equilibrium between RanBP2, HKI, and Cox11. This pathophysiological event promotes the destabilization and degradation of HKI and a decrease in ATP production required to maintain the depolarization state neurons, and, hence, a reduction in the response of receptoral and postreceptoral neurons. A reduction in ATP levels also negatively modulates HKI activity/level. Decreased levels of HKI promote intracellular hyperglycemia and activate stress kinases, which modulate negatively the Na+/K+-ATPase pump by phosphorylation. Pathophysiological pathways promoted by RanBP2 haploinsufficiency are represented by dash lines. RIS, rod inner segment; ROS, rod outer segment. Finally
T14525	38445-38450	NN	denotes	Model
T14526	38451-38460	VBG	denotes	Depicting
T14527	38461-38462	DT	denotes	a
T14528	38463-38467	NN	denotes	Role
T14529	38468-38470	IN	denotes	of
T14530	38471-38477	NN	denotes	RanBP2
T14531	38478-38481	CC	denotes	and
T14532	38482-38486	DT	denotes	Some
T14533	38487-38489	IN	denotes	of
T14534	38490-38493	PRP$	denotes	Its
T14535	38494-38502	NNS	denotes	Partners
T14536	38503-38505	IN	denotes	in
T14537	38506-38515	JJ	denotes	Metabolic
T14539	38516-38519	CC	denotes	and
T14540	38520-38528	JJ	denotes	Neuronal
T14538	38529-38537	NN	denotes	Function
T14541	38537-38644	sentence	denotes	RanBP2 interacts with Cox11 and HKI and the triad is in equilibrium under normal physiological conditions.
T14542	38538-38544	NN	denotes	RanBP2
T14543	38545-38554	VBZ	denotes	interacts
T14544	38555-38559	IN	denotes	with
T14545	38560-38565	NN	denotes	Cox11
T14546	38566-38569	CC	denotes	and
T14547	38570-38573	NN	denotes	HKI
T14548	38574-38577	CC	denotes	and
T14549	38578-38581	DT	denotes	the
T14550	38582-38587	NN	denotes	triad
T14551	38588-38590	VBZ	denotes	is
T14552	38591-38593	IN	denotes	in
T14553	38594-38605	NN	denotes	equilibrium
T14554	38606-38611	IN	denotes	under
T14555	38612-38618	JJ	denotes	normal
T14557	38619-38632	JJ	denotes	physiological
T14556	38633-38643	NNS	denotes	conditions
T14558	38643-38644	.	denotes	.
T14559	38644-38712	sentence	denotes	RanBP2 prevents the inhibition of HKI by Cox11 and its degradation.
T14560	38645-38651	NN	denotes	RanBP2
T14561	38652-38660	VBZ	denotes	prevents
T14562	38661-38664	DT	denotes	the
T14563	38665-38675	NN	denotes	inhibition
T14564	38676-38678	IN	denotes	of
T14565	38679-38682	NN	denotes	HKI
T14566	38683-38685	IN	denotes	by
T14567	38686-38691	NN	denotes	Cox11
T14568	38692-38695	CC	denotes	and
T14569	38696-38699	PRP$	denotes	its
T14570	38700-38711	NN	denotes	degradation
T14571	38711-38712	.	denotes	.
T14572	38712-38826	sentence	denotes	The ultimate effect of RanBP2 on its partners is the stimulation of the glycolytic pathway and production of ATP.
T14573	38713-38716	DT	denotes	The
T14575	38717-38725	JJ	denotes	ultimate
T14574	38726-38732	NN	denotes	effect
T14577	38733-38735	IN	denotes	of
T14578	38736-38742	NN	denotes	RanBP2
T14579	38743-38745	IN	denotes	on
T14580	38746-38749	PRP$	denotes	its
T14581	38750-38758	NNS	denotes	partners
T14576	38759-38761	VBZ	denotes	is
T14582	38762-38765	DT	denotes	the
T14583	38766-38777	NN	denotes	stimulation
T14584	38778-38780	IN	denotes	of
T14585	38781-38784	DT	denotes	the
T14587	38785-38795	JJ	denotes	glycolytic
T14586	38796-38803	NN	denotes	pathway
T14588	38804-38807	CC	denotes	and
T14589	38808-38818	NN	denotes	production
T14590	38819-38821	IN	denotes	of
T14591	38822-38825	NN	denotes	ATP
T14592	38825-38826	.	denotes	.
T14593	38826-39008	sentence	denotes	The glycolytic pathway is critical to fuel the constitutive Na+/K+-ATPase pump to maintain the dark current between the inner and outer segment compartments of photosensory neurons.
T14594	38827-38830	DT	denotes	The
T14596	38831-38841	JJ	denotes	glycolytic
T14595	38842-38849	NN	denotes	pathway
T14597	38850-38852	VBZ	denotes	is
T14598	38853-38861	JJ	denotes	critical
T14599	38862-38864	TO	denotes	to
T14600	38865-38869	VB	denotes	fuel
T14601	38870-38873	DT	denotes	the
T14603	38874-38886	JJ	denotes	constitutive
T14604	38887-38890	NN	denotes	Na+
T14606	38890-38891	SYM	denotes	/
T14605	38891-38893	NN	denotes	K+
T14608	38893-38894	HYPH	denotes	-
T14607	38894-38900	NN	denotes	ATPase
T14602	38901-38905	NN	denotes	pump
T14609	38906-38908	TO	denotes	to
T14610	38909-38917	VB	denotes	maintain
T14611	38918-38921	DT	denotes	the
T14613	38922-38926	JJ	denotes	dark
T14612	38927-38934	NN	denotes	current
T14614	38935-38942	IN	denotes	between
T14615	38943-38946	DT	denotes	the
T14617	38947-38952	JJ	denotes	inner
T14618	38953-38956	CC	denotes	and
T14619	38957-38962	JJ	denotes	outer
T14620	38963-38970	NN	denotes	segment
T14616	38971-38983	NNS	denotes	compartments
T14621	38984-38986	IN	denotes	of
T14622	38987-38999	JJ	denotes	photosensory
T14623	39000-39007	NNS	denotes	neurons
T14624	39007-39008	.	denotes	.
T14625	39008-39106	sentence	denotes	A deficit (haploinsufficiency) in RanBP2 disturbs the equilibrium between RanBP2, HKI, and Cox11.
T14626	39009-39010	DT	denotes	A
T14627	39011-39018	NN	denotes	deficit
T14629	39019-39020	-LRB-	denotes	(
T14630	39020-39038	NN	denotes	haploinsufficiency
T14631	39038-39039	-RRB-	denotes	)
T14632	39040-39042	IN	denotes	in
T14633	39043-39049	NN	denotes	RanBP2
T14628	39050-39058	VBZ	denotes	disturbs
T14634	39059-39062	DT	denotes	the
T14635	39063-39074	NN	denotes	equilibrium
T14636	39075-39082	IN	denotes	between
T14637	39083-39089	NN	denotes	RanBP2
T14638	39089-39091	,	denotes	,
T14639	39091-39094	NN	denotes	HKI
T14640	39094-39096	,	denotes	,
T14641	39096-39099	CC	denotes	and
T14642	39100-39105	NN	denotes	Cox11
T14643	39105-39106	.	denotes	.
T14644	39106-39358	sentence	denotes	This pathophysiological event promotes the destabilization and degradation of HKI and a decrease in ATP production required to maintain the depolarization state neurons, and, hence, a reduction in the response of receptoral and postreceptoral neurons.
T14645	39107-39111	DT	denotes	This
T14647	39112-39130	JJ	denotes	pathophysiological
T14646	39131-39136	NN	denotes	event
T14648	39137-39145	VBZ	denotes	promotes
T14649	39146-39149	DT	denotes	the
T14650	39150-39165	NN	denotes	destabilization
T14651	39166-39169	CC	denotes	and
T14652	39170-39181	NN	denotes	degradation
T14653	39182-39184	IN	denotes	of
T14654	39185-39188	NN	denotes	HKI
T14655	39189-39192	CC	denotes	and
T14656	39193-39194	DT	denotes	a
T14657	39195-39203	NN	denotes	decrease
T14658	39204-39206	IN	denotes	in
T14659	39207-39210	NN	denotes	ATP
T14660	39211-39221	NN	denotes	production
T14661	39222-39230	VBN	denotes	required
T14662	39231-39233	TO	denotes	to
T14663	39234-39242	VB	denotes	maintain
T14664	39243-39246	DT	denotes	the
T14666	39247-39261	NN	denotes	depolarization
T14667	39262-39267	NN	denotes	state
T14665	39268-39275	NNS	denotes	neurons
T14668	39275-39277	,	denotes	,
T14669	39277-39280	CC	denotes	and
T14670	39280-39282	,	denotes	,
T14671	39282-39287	RB	denotes	hence
T14673	39287-39289	,	denotes	,
T14674	39289-39290	DT	denotes	a
T14672	39291-39300	NN	denotes	reduction
T14675	39301-39303	IN	denotes	in
T14676	39304-39307	DT	denotes	the
T14677	39308-39316	NN	denotes	response
T14678	39317-39319	IN	denotes	of
T14679	39320-39330	JJ	denotes	receptoral
T14681	39331-39334	CC	denotes	and
T14682	39335-39349	JJ	denotes	postreceptoral
T14680	39350-39357	NNS	denotes	neurons
T14683	39357-39358	.	denotes	.
T14684	39358-39430	sentence	denotes	A reduction in ATP levels also negatively modulates HKI activity/level.
T14685	39359-39360	DT	denotes	A
T14686	39361-39370	NN	denotes	reduction
T14688	39371-39373	IN	denotes	in
T14689	39374-39377	NN	denotes	ATP
T14690	39378-39384	NNS	denotes	levels
T14691	39385-39389	RB	denotes	also
T14692	39390-39400	RB	denotes	negatively
T14687	39401-39410	VBZ	denotes	modulates
T14693	39411-39414	NN	denotes	HKI
T14694	39415-39423	NN	denotes	activity
T14695	39423-39424	SYM	denotes	/
T14696	39424-39429	NN	denotes	level
T14697	39429-39430	.	denotes	.
T14698	39430-39588	sentence	denotes	Decreased levels of HKI promote intracellular hyperglycemia and activate stress kinases, which modulate negatively the Na+/K+-ATPase pump by phosphorylation.
T14699	39431-39440	VBN	denotes	Decreased
T14700	39441-39447	NNS	denotes	levels
T14702	39448-39450	IN	denotes	of
T14703	39451-39454	NN	denotes	HKI
T14701	39455-39462	VBP	denotes	promote
T14704	39463-39476	JJ	denotes	intracellular
T14705	39477-39490	NN	denotes	hyperglycemia
T14706	39491-39494	CC	denotes	and
T14707	39495-39503	VBP	denotes	activate
T14708	39504-39510	NN	denotes	stress
T14709	39511-39518	NNS	denotes	kinases
T14710	39518-39520	,	denotes	,
T14711	39520-39525	WDT	denotes	which
T14712	39526-39534	VBP	denotes	modulate
T14713	39535-39545	RB	denotes	negatively
T14714	39546-39549	DT	denotes	the
T14716	39550-39553	NN	denotes	Na+
T14718	39553-39554	HYPH	denotes	/
T14717	39554-39556	NN	denotes	K+
T14720	39556-39557	HYPH	denotes	-
T14719	39557-39563	NN	denotes	ATPase
T14715	39564-39568	NN	denotes	pump
T14721	39569-39571	IN	denotes	by
T14722	39572-39587	NN	denotes	phosphorylation
T14723	39587-39588	.	denotes	.
T14724	39588-39685	sentence	denotes	Pathophysiological pathways promoted by RanBP2 haploinsufficiency are represented by dash lines.
T14725	39589-39607	JJ	denotes	Pathophysiological
T14726	39608-39616	NNS	denotes	pathways
T14728	39617-39625	VBN	denotes	promoted
T14729	39626-39628	IN	denotes	by
T14730	39629-39635	NN	denotes	RanBP2
T14731	39636-39654	NN	denotes	haploinsufficiency
T14732	39655-39658	VBP	denotes	are
T14727	39659-39670	VBN	denotes	represented
T14733	39671-39673	IN	denotes	by
T14734	39674-39678	NN	denotes	dash
T14735	39679-39684	NNS	denotes	lines
T14736	39684-39685	.	denotes	.
T14737	39685-39733	sentence	denotes	RIS, rod inner segment; ROS, rod outer segment.
T14738	39686-39689	NN	denotes	RIS
T14739	39689-39691	,	denotes	,
T14740	39691-39694	NN	denotes	rod
T14742	39695-39700	JJ	denotes	inner
T14741	39701-39708	NN	denotes	segment
T14743	39708-39709	:	denotes	;
T14744	39710-39713	NN	denotes	ROS
T14745	39713-39715	,	denotes	,
T14746	39715-39718	NN	denotes	rod
T14748	39719-39724	JJ	denotes	outer
T14747	39725-39732	NN	denotes	segment
T14749	39732-39733	.	denotes	.
T7457	39741-39743	,	denotes	,
T7458	39743-39749	NN	denotes	RanBP2
T7456	39750-39757	VBZ	denotes	appears
T7459	39758-39760	TO	denotes	to
T7460	39761-39765	VB	denotes	join
T7461	39766-39771	JJ	denotes	other
T7462	39772-39784	NNS	denotes	nucleoporins
T7463	39784-39786	,	denotes	,
T7464	39786-39790	JJ	denotes	such
T7465	39791-39793	IN	denotes	as
T7466	39794-39799	NN	denotes	Nup96
T7467	39800-39801	-LRB-	denotes	[
T7468	39801-39803	CD	denotes	58
T7469	39803-39804	-RRB-	denotes	]
T7470	39804-39806	,	denotes	,
T7471	39806-39808	IN	denotes	in
T7472	39809-39814	JJ	denotes	novel
T7474	39815-39828	JJ	denotes	physiological
T7473	39829-39838	NNS	denotes	functions
T7475	39839-39843	WDT	denotes	that
T7476	39844-39847	VBP	denotes	are
T7477	39848-39853	JJ	denotes	vital
T7478	39854-39857	IN	denotes	for
T7479	39858-39865	JJ	denotes	complex
T7480	39866-39875	NNS	denotes	organisms
T7481	39876-39879	CC	denotes	and
T7482	39880-39884	WDT	denotes	that
T7484	39885-39889	VBD	denotes	were
T7485	39890-39893	RB	denotes	not
T7483	39894-39905	VBN	denotes	anticipated
T7486	39906-39910	IN	denotes	from
T7487	39911-39915	NN	denotes	cell
T7489	39915-39916	HYPH	denotes	-
T7488	39916-39921	VBN	denotes	based
T7490	39922-39929	NNS	denotes	studies
T7491	39929-39930	.	denotes	.
T7492	39930-40169	sentence	denotes	The reduced expression of these proteins in whole-animal models generates phenotypes that are tissue-restricted and more importantly, do not seem to recapitulate phenotypes observed with knockdown experiments in cell-based culture assays.
T7493	39931-39934	DT	denotes	The
T7495	39935-39942	VBN	denotes	reduced
T7494	39943-39953	NN	denotes	expression
T7497	39954-39956	IN	denotes	of
T7498	39957-39962	DT	denotes	these
T7499	39963-39971	NN	denotes	proteins
T7500	39972-39974	IN	denotes	in
T7501	39975-39980	JJ	denotes	whole
T7503	39980-39981	HYPH	denotes	-
T7502	39981-39987	NN	denotes	animal
T7504	39988-39994	NNS	denotes	models
T7496	39995-40004	VBZ	denotes	generates
T7505	40005-40015	NNS	denotes	phenotypes
T7506	40016-40020	WDT	denotes	that
T7507	40021-40024	VBP	denotes	are
T7508	40025-40031	NN	denotes	tissue
T7510	40031-40032	HYPH	denotes	-
T7509	40032-40042	JJ	denotes	restricted
T7511	40043-40046	CC	denotes	and
T7512	40047-40051	RBR	denotes	more
T7513	40052-40063	RB	denotes	importantly
T7515	40063-40065	,	denotes	,
T7516	40065-40067	VBP	denotes	do
T7517	40068-40071	RB	denotes	not
T7514	40072-40076	VB	denotes	seem
T7518	40077-40079	TO	denotes	to
T7519	40080-40092	VB	denotes	recapitulate
T7520	40093-40103	NNS	denotes	phenotypes
T7521	40104-40112	VBN	denotes	observed
T7522	40113-40117	IN	denotes	with
T7523	40118-40127	NN	denotes	knockdown
T7524	40128-40139	NNS	denotes	experiments
T7525	40140-40142	IN	denotes	in
T7526	40143-40147	NN	denotes	cell
T7528	40147-40148	HYPH	denotes	-
T7527	40148-40153	VBN	denotes	based
T7530	40154-40161	NN	denotes	culture
T7529	40162-40168	NNS	denotes	assays
T7531	40168-40169	.	denotes	.
T7532	40169-40421	sentence	denotes	For example, short interfering RNA-mediated knockdown of RanBP2 [9] and members of the Nup107-Nup160 complex [59–61] have shown, respectively, to cause mitotic arrest and disruption of nuclear pore assembly and deficits in mRNA export in cell culture.
T7533	40170-40173	IN	denotes	For
T7535	40174-40181	NN	denotes	example
T7536	40181-40183	,	denotes	,
T7537	40183-40188	JJ	denotes	short
T7539	40189-40200	VBG	denotes	interfering
T7540	40201-40204	NN	denotes	RNA
T7542	40204-40205	HYPH	denotes	-
T7541	40205-40213	VBN	denotes	mediated
T7538	40214-40223	NN	denotes	knockdown
T7543	40224-40226	IN	denotes	of
T7544	40227-40233	NN	denotes	RanBP2
T7545	40234-40235	-LRB-	denotes	[
T7546	40235-40236	CD	denotes	9
T7547	40236-40237	-RRB-	denotes	]
T7548	40238-40241	CC	denotes	and
T7549	40242-40249	NNS	denotes	members
T7550	40250-40252	IN	denotes	of
T7551	40253-40256	DT	denotes	the
T7553	40257-40263	NN	denotes	Nup107
T7555	40263-40264	HYPH	denotes	-
T7554	40264-40270	NN	denotes	Nup160
T7552	40271-40278	NN	denotes	complex
T7556	40279-40280	-LRB-	denotes	[
T7557	40280-40282	CD	denotes	59
T7558	40282-40283	SYM	denotes	–
T7559	40283-40285	CD	denotes	61
T7560	40285-40286	-RRB-	denotes	]
T7561	40287-40291	VBP	denotes	have
T7534	40292-40297	VBN	denotes	shown
T7562	40297-40299	,	denotes	,
T7563	40299-40311	RB	denotes	respectively
T7564	40311-40313	,	denotes	,
T7565	40313-40315	TO	denotes	to
T7566	40316-40321	VB	denotes	cause
T7567	40322-40329	JJ	denotes	mitotic
T7568	40330-40336	NN	denotes	arrest
T7569	40337-40340	CC	denotes	and
T7570	40341-40351	NN	denotes	disruption
T7571	40352-40354	IN	denotes	of
T7572	40355-40362	JJ	denotes	nuclear
T7573	40363-40367	NN	denotes	pore
T7574	40368-40376	NN	denotes	assembly
T7575	40377-40380	CC	denotes	and
T7576	40381-40389	NNS	denotes	deficits
T7577	40390-40392	IN	denotes	in
T7578	40393-40397	NN	denotes	mRNA
T7579	40398-40404	NN	denotes	export
T7580	40405-40407	IN	denotes	in
T7581	40408-40412	NN	denotes	cell
T7582	40413-40420	NN	denotes	culture
T7583	40420-40421	.	denotes	.
T7584	40421-40724	sentence	denotes	Yet, haploinsufficiency of RanBP2 (this work) and Nup96 [58] predominantly produce, instead, CNS-restricted deficits in energy metabolism and alterations in the immune system linked to the down-regulation of interferon-β-regulated proteins and increased susceptibility of viral infection, respectively.
T7585	40422-40425	RB	denotes	Yet
T7587	40425-40427	,	denotes	,
T7588	40427-40445	NN	denotes	haploinsufficiency
T7589	40446-40448	IN	denotes	of
T7590	40449-40455	NN	denotes	RanBP2
T7591	40456-40457	-LRB-	denotes	(
T7593	40457-40461	DT	denotes	this
T7592	40462-40466	NN	denotes	work
T7594	40466-40467	-RRB-	denotes	)
T7595	40468-40471	CC	denotes	and
T7596	40472-40477	NN	denotes	Nup96
T7597	40478-40479	-LRB-	denotes	[
T7598	40479-40481	CD	denotes	58
T7599	40481-40482	-RRB-	denotes	]
T7600	40483-40496	RB	denotes	predominantly
T7586	40497-40504	VBP	denotes	produce
T7601	40504-40506	,	denotes	,
T7602	40506-40513	RB	denotes	instead
T7603	40513-40515	,	denotes	,
T7604	40515-40518	NN	denotes	CNS
T7606	40518-40519	HYPH	denotes	-
T7605	40519-40529	JJ	denotes	restricted
T7607	40530-40538	NNS	denotes	deficits
T7608	40539-40541	IN	denotes	in
T7609	40542-40548	NN	denotes	energy
T7610	40549-40559	NN	denotes	metabolism
T7611	40560-40563	CC	denotes	and
T7612	40564-40575	NNS	denotes	alterations
T7613	40576-40578	IN	denotes	in
T7614	40579-40582	DT	denotes	the
T7616	40583-40589	JJ	denotes	immune
T7615	40590-40596	NN	denotes	system
T7617	40597-40603	VBN	denotes	linked
T7618	40604-40606	IN	denotes	to
T7619	40607-40610	DT	denotes	the
T7621	40611-40615	JJ	denotes	down
T7622	40615-40616	HYPH	denotes	-
T7620	40616-40626	NN	denotes	regulation
T7623	40627-40629	IN	denotes	of
T7624	40630-40640	NN	denotes	interferon
T7626	40640-40641	HYPH	denotes	-
T7625	40641-40642	NN	denotes	β
T7628	40642-40643	HYPH	denotes	-
T7627	40643-40652	VBN	denotes	regulated
T7629	40653-40661	NN	denotes	proteins
T7630	40662-40665	CC	denotes	and
T7631	40666-40675	VBN	denotes	increased
T7632	40676-40690	NN	denotes	susceptibility
T7633	40691-40693	IN	denotes	of
T7634	40694-40699	JJ	denotes	viral
T7635	40700-40709	NN	denotes	infection
T7636	40709-40711	,	denotes	,
T7637	40711-40723	RB	denotes	respectively
T7638	40723-40724	.	denotes	.
T7639	40724-40936	sentence	denotes	While these apparent outcome disparities among experimental systems remain unclear, they could potentially result from variations in redundancies and compensatory mechanisms inherent to each experimental system.
T7640	40725-40730	IN	denotes	While
T7642	40731-40736	DT	denotes	these
T7644	40737-40745	JJ	denotes	apparent
T7645	40746-40753	NN	denotes	outcome
T7643	40754-40765	NNS	denotes	disparities
T7646	40766-40771	IN	denotes	among
T7647	40772-40784	JJ	denotes	experimental
T7648	40785-40792	NNS	denotes	systems
T7641	40793-40799	VBP	denotes	remain
T7650	40800-40807	JJ	denotes	unclear
T7651	40807-40809	,	denotes	,
T7652	40809-40813	PRP	denotes	they
T7653	40814-40819	MD	denotes	could
T7654	40820-40831	RB	denotes	potentially
T7649	40832-40838	VB	denotes	result
T7655	40839-40843	IN	denotes	from
T7656	40844-40854	NNS	denotes	variations
T7657	40855-40857	IN	denotes	in
T7658	40858-40870	NNS	denotes	redundancies
T7659	40871-40874	CC	denotes	and
T7660	40875-40887	JJ	denotes	compensatory
T7661	40888-40898	NNS	denotes	mechanisms
T7662	40899-40907	JJ	denotes	inherent
T7663	40908-40910	IN	denotes	to
T7664	40911-40915	DT	denotes	each
T7666	40916-40928	JJ	denotes	experimental
T7665	40929-40935	NN	denotes	system
T7667	40935-40936	.	denotes	.
T7668	40936-41172	sentence	denotes	Regardless, these genetic and whole-animal models set the stage to probe novel molecular pathways in various physiological and genetic contexts, and provide also a link to pathophysiological processes underlying several human diseases.
T7669	40937-40947	RB	denotes	Regardless
T7671	40947-40949	,	denotes	,
T7672	40949-40954	DT	denotes	these
T7674	40955-40962	JJ	denotes	genetic
T7675	40963-40966	CC	denotes	and
T7676	40967-40972	JJ	denotes	whole
T7678	40972-40973	HYPH	denotes	-
T7677	40973-40979	NN	denotes	animal
T7673	40980-40986	NNS	denotes	models
T7670	40987-40990	VBD	denotes	set
T7679	40991-40994	DT	denotes	the
T7680	40995-41000	NN	denotes	stage
T7681	41001-41003	TO	denotes	to
T7682	41004-41009	VB	denotes	probe
T7683	41010-41015	JJ	denotes	novel
T7685	41016-41025	JJ	denotes	molecular
T7684	41026-41034	NNS	denotes	pathways
T7686	41035-41037	IN	denotes	in
T7687	41038-41045	JJ	denotes	various
T7689	41046-41059	JJ	denotes	physiological
T7690	41060-41063	CC	denotes	and
T7691	41064-41071	JJ	denotes	genetic
T7688	41072-41080	NNS	denotes	contexts
T7692	41080-41082	,	denotes	,
T7693	41082-41085	CC	denotes	and
T7694	41086-41093	VB	denotes	provide
T7695	41094-41098	RB	denotes	also
T7696	41099-41100	DT	denotes	a
T7697	41101-41105	NN	denotes	link
T7698	41106-41108	IN	denotes	to
T7699	41109-41127	JJ	denotes	pathophysiological
T7700	41128-41137	NNS	denotes	processes
T7701	41138-41148	VBG	denotes	underlying
T7702	41149-41156	JJ	denotes	several
T7704	41157-41162	JJ	denotes	human
T7703	41163-41171	NNS	denotes	diseases
T7705	41171-41172	.	denotes	.
T7787	41197-41202	NN	denotes	Yeast
T7789	41203-41206	CD	denotes	two
T7791	41206-41207	HYPH	denotes	-
T7790	41207-41213	NN	denotes	hybrid
T7788	41214-41223	NN	denotes	screening
T7792	41224-41227	CC	denotes	and
T7793	41228-41234	NNS	denotes	assays
T7794	41234-41235	.	denotes	.
T7795	41235-41499	sentence	denotes	The LD of human RanBP2 (residues 62–711) was subcloned in-frame into the GAL4 DNA-binding domain of bait vector, pBUTE (a kanamycin-resistant version of GAL4 bait vector pGBDUC1) [62] and HybriZAP pBD-GAL4 vector (Stratagene, La Jolla, California, United States).
T7796	41236-41239	DT	denotes	The
T7797	41240-41242	NN	denotes	LD
T7799	41243-41245	IN	denotes	of
T7800	41246-41251	JJ	denotes	human
T7801	41252-41258	NN	denotes	RanBP2
T7802	41259-41260	-LRB-	denotes	(
T7804	41260-41268	NNS	denotes	residues
T7803	41269-41271	CD	denotes	62
T7805	41271-41272	SYM	denotes	–
T7806	41272-41275	CD	denotes	711
T7807	41275-41276	-RRB-	denotes	)
T7808	41277-41280	VBD	denotes	was
T7798	41281-41290	VBN	denotes	subcloned
T7809	41291-41293	IN	denotes	in
T7810	41293-41294	HYPH	denotes	-
T7811	41294-41299	NN	denotes	frame
T7812	41300-41304	IN	denotes	into
T7813	41305-41308	DT	denotes	the
T7815	41309-41313	NN	denotes	GAL4
T7816	41314-41317	NN	denotes	DNA
T7818	41317-41318	HYPH	denotes	-
T7817	41318-41325	NN	denotes	binding
T7814	41326-41332	NN	denotes	domain
T7819	41333-41335	IN	denotes	of
T7820	41336-41340	NN	denotes	bait
T7821	41341-41347	NN	denotes	vector
T7822	41347-41349	,	denotes	,
T7823	41349-41354	NN	denotes	pBUTE
T7824	41355-41356	-LRB-	denotes	(
T7825	41356-41357	DT	denotes	a
T7827	41358-41367	NN	denotes	kanamycin
T7829	41367-41368	HYPH	denotes	-
T7828	41368-41377	JJ	denotes	resistant
T7826	41378-41385	NN	denotes	version
T7830	41386-41388	IN	denotes	of
T7831	41389-41393	NN	denotes	GAL4
T7833	41394-41398	NN	denotes	bait
T7832	41399-41405	NN	denotes	vector
T7834	41406-41413	NN	denotes	pGBDUC1
T7835	41413-41414	-RRB-	denotes	)
T7836	41415-41416	-LRB-	denotes	[
T7837	41416-41418	CD	denotes	62
T7838	41418-41419	-RRB-	denotes	]
T7839	41420-41423	CC	denotes	and
T7840	41424-41432	NN	denotes	HybriZAP
T7842	41433-41436	NN	denotes	pBD
T7844	41436-41437	HYPH	denotes	-
T7843	41437-41441	NN	denotes	GAL4
T7841	41442-41448	NN	denotes	vector
T7845	41449-41450	-LRB-	denotes	(
T7846	41450-41460	NNP	denotes	Stratagene
T7847	41460-41462	,	denotes	,
T7848	41462-41464	NNP	denotes	La
T7849	41465-41470	NNP	denotes	Jolla
T7850	41470-41472	,	denotes	,
T7851	41472-41482	NNP	denotes	California
T7852	41482-41484	,	denotes	,
T7853	41484-41490	NNP	denotes	United
T7854	41491-41497	NNP	denotes	States
T7855	41497-41498	-RRB-	denotes	)
T7856	41498-41499	.	denotes	.
T7857	41499-41708	sentence	denotes	The former was used to screen ~18 million clones via mating from murine 9- to 10-d-old embryo and brain cDNA libraries at the Molecular Interaction Facility (MIF), University of Wisconsin, Madison, Wisconsin.
T7858	41500-41503	DT	denotes	The
T7859	41504-41510	JJ	denotes	former
T7861	41511-41514	VBD	denotes	was
T7860	41515-41519	VBN	denotes	used
T7862	41520-41522	TO	denotes	to
T7863	41523-41529	VB	denotes	screen
T7864	41530-41531	SYM	denotes	~
T7866	41531-41533	CD	denotes	18
T7865	41534-41541	CD	denotes	million
T7867	41542-41548	NNS	denotes	clones
T7868	41549-41552	IN	denotes	via
T7869	41553-41559	VBG	denotes	mating
T7870	41560-41564	IN	denotes	from
T7871	41565-41571	NN	denotes	murine
T7873	41572-41573	CD	denotes	9
T7875	41573-41574	HYPH	denotes	-
T7876	41575-41577	IN	denotes	to
T7874	41578-41580	CD	denotes	10
T7878	41580-41581	HYPH	denotes	-
T7877	41581-41582	NN	denotes	d
T7880	41582-41583	HYPH	denotes	-
T7879	41583-41586	JJ	denotes	old
T7881	41587-41593	NN	denotes	embryo
T7882	41594-41597	CC	denotes	and
T7883	41598-41603	NN	denotes	brain
T7884	41604-41608	NN	denotes	cDNA
T7872	41609-41618	NNS	denotes	libraries
T7885	41619-41621	IN	denotes	at
T7886	41622-41625	DT	denotes	the
T7888	41626-41635	NNP	denotes	Molecular
T7889	41636-41647	NNP	denotes	Interaction
T7887	41648-41656	NNP	denotes	Facility
T7890	41657-41658	-LRB-	denotes	(
T7891	41658-41661	NNP	denotes	MIF
T7892	41661-41662	-RRB-	denotes	)
T7893	41662-41664	,	denotes	,
T7894	41664-41674	NNP	denotes	University
T7895	41675-41677	IN	denotes	of
T7896	41678-41687	NNP	denotes	Wisconsin
T7897	41687-41689	,	denotes	,
T7898	41689-41696	NNP	denotes	Madison
T7899	41696-41698	,	denotes	,
T7900	41698-41707	NNP	denotes	Wisconsin
T7901	41707-41708	.	denotes	.
T7902	41708-41819	sentence	denotes	The latter was used to screen via transformation of ~5 million clones from bovine retinal cDNA libraries [32].
T7903	41709-41712	DT	denotes	The
T7904	41713-41719	JJ	denotes	latter
T7906	41720-41723	VBD	denotes	was
T7905	41724-41728	VBN	denotes	used
T7907	41729-41731	TO	denotes	to
T7908	41732-41738	VB	denotes	screen
T7909	41739-41742	IN	denotes	via
T7910	41743-41757	NN	denotes	transformation
T7911	41758-41760	IN	denotes	of
T7912	41761-41762	SYM	denotes	~
T7914	41762-41763	CD	denotes	5
T7913	41764-41771	CD	denotes	million
T7915	41772-41778	NNS	denotes	clones
T7916	41779-41783	IN	denotes	from
T7917	41784-41790	JJ	denotes	bovine
T7919	41791-41798	JJ	denotes	retinal
T7920	41799-41803	NN	denotes	cDNA
T7918	41804-41813	NNS	denotes	libraries
T7921	41814-41815	-LRB-	denotes	[
T7922	41815-41817	CD	denotes	32
T7923	41817-41818	-RRB-	denotes	]
T7924	41818-41819	.	denotes	.
T7925	41819-41853	sentence	denotes	The screens generated six clones.
T7926	41820-41823	DT	denotes	The
T7927	41824-41831	NNS	denotes	screens
T7928	41832-41841	VBD	denotes	generated
T7929	41842-41845	CD	denotes	six
T7930	41846-41852	NNS	denotes	clones
T7931	41852-41853	.	denotes	.
T7932	41853-42006	sentence	denotes	One and two in-frame clones were independently isolated from the embryonic and adult brain libraries, respectively, and the interactions were validated.
T7933	41854-41857	CD	denotes	One
T7935	41858-41861	CC	denotes	and
T7936	41862-41865	CD	denotes	two
T7937	41866-41868	IN	denotes	in
T7938	41868-41869	HYPH	denotes	-
T7939	41869-41874	NN	denotes	frame
T7934	41875-41881	NNS	denotes	clones
T7941	41882-41886	VBD	denotes	were
T7942	41887-41900	RB	denotes	independently
T7940	41901-41909	VBN	denotes	isolated
T7943	41910-41914	IN	denotes	from
T7944	41915-41918	DT	denotes	the
T7946	41919-41928	JJ	denotes	embryonic
T7947	41929-41932	CC	denotes	and
T7948	41933-41938	JJ	denotes	adult
T7949	41939-41944	NN	denotes	brain
T7945	41945-41954	NNS	denotes	libraries
T7950	41954-41956	,	denotes	,
T7951	41956-41968	RB	denotes	respectively
T7952	41968-41970	,	denotes	,
T7953	41970-41973	CC	denotes	and
T7954	41974-41977	DT	denotes	the
T7955	41978-41990	NNS	denotes	interactions
T7957	41991-41995	VBD	denotes	were
T7956	41996-42005	VBN	denotes	validated
T7958	42005-42006	.	denotes	.
T7959	42006-42038	sentence	denotes	The three clones encoded Cox11.
T7960	42007-42010	DT	denotes	The
T7962	42011-42016	CD	denotes	three
T7961	42017-42023	NNS	denotes	clones
T7963	42024-42031	VBD	denotes	encoded
T7964	42032-42037	NN	denotes	Cox11
T7965	42037-42038	.	denotes	.
T7966	42038-42220	sentence	denotes	Interactions between Cox11, LD, and subdomains thereof were quantified by liquid β-galactosidase (Applied Biosystems, Foster City, California, United States) and growth assays [63].
T7967	42039-42051	NNS	denotes	Interactions
T7969	42052-42059	IN	denotes	between
T7970	42060-42065	NN	denotes	Cox11
T7971	42065-42067	,	denotes	,
T7972	42067-42069	NN	denotes	LD
T7973	42069-42071	,	denotes	,
T7974	42071-42074	CC	denotes	and
T7975	42075-42085	NNS	denotes	subdomains
T7976	42086-42093	RB	denotes	thereof
T7977	42094-42098	VBD	denotes	were
T7968	42099-42109	VBN	denotes	quantified
T7978	42110-42112	IN	denotes	by
T7979	42113-42119	JJ	denotes	liquid
T7981	42120-42121	NN	denotes	β
T7982	42121-42122	HYPH	denotes	-
T7980	42122-42135	NN	denotes	galactosidase
T7983	42136-42137	-LRB-	denotes	(
T7985	42137-42144	NNP	denotes	Applied
T7984	42145-42155	NNP	denotes	Biosystems
T7986	42155-42157	,	denotes	,
T7987	42157-42163	NNP	denotes	Foster
T7988	42164-42168	NNP	denotes	City
T7989	42168-42170	,	denotes	,
T7990	42170-42180	NNP	denotes	California
T7991	42180-42182	,	denotes	,
T7992	42182-42188	NNP	denotes	United
T7993	42189-42195	NNP	denotes	States
T7994	42195-42196	-RRB-	denotes	)
T7995	42197-42200	CC	denotes	and
T7996	42201-42207	NN	denotes	growth
T7997	42208-42214	NNS	denotes	assays
T7998	42215-42216	-LRB-	denotes	[
T7999	42216-42218	CD	denotes	63
T8000	42218-42219	-RRB-	denotes	]
T8001	42219-42220	.	denotes	.
T8002	42220-42434	sentence	denotes	The maximum specific growth speed (μmax) was determined by calculating μmax = (ln(x t) − ln(x 0))/t, where x t is the OD600 of the culture at t = t, x 0 is the OD600 at t = 0 and t is the time between x 0 and x t.
T8003	42221-42224	DT	denotes	The
T8005	42225-42232	NN	denotes	maximum
T8006	42233-42241	JJ	denotes	specific
T8007	42242-42248	NN	denotes	growth
T8004	42249-42254	NN	denotes	speed
T8009	42255-42256	-LRB-	denotes	(
T8010	42256-42260	NN	denotes	μmax
T8011	42260-42261	-RRB-	denotes	)
T8012	42262-42265	VBD	denotes	was
T8008	42266-42276	VBN	denotes	determined
T8013	42277-42279	IN	denotes	by
T8014	42280-42291	VBG	denotes	calculating
T8015	42292-42296	NN	denotes	μmax
T8017	42297-42298	SYM	denotes	=
T8018	42299-42300	-LRB-	denotes	(
T8019	42300-42302	NN	denotes	ln
T8020	42302-42303	-LRB-	denotes	(
T8016	42303-42306	NN	denotes	x t
T8021	42306-42307	-RRB-	denotes	)
T8022	42308-42309	SYM	denotes	−
T8023	42310-42312	NN	denotes	ln
T8025	42312-42313	-LRB-	denotes	(
T8024	42313-42316	NN	denotes	x 0
T8026	42316-42317	-RRB-	denotes	)
T8027	42317-42318	-RRB-	denotes	)
T8028	42318-42319	SYM	denotes	/
T8029	42319-42320	NN	denotes	t
T8030	42320-42322	,	denotes	,
T8031	42322-42327	WRB	denotes	where
T8033	42328-42331	NN	denotes	x t
T8032	42332-42334	VBZ	denotes	is
T8034	42335-42338	DT	denotes	the
T8035	42339-42344	NN	denotes	OD600
T8036	42345-42347	IN	denotes	of
T8037	42348-42351	DT	denotes	the
T8038	42352-42359	NN	denotes	culture
T8039	42360-42362	IN	denotes	at
T8040	42363-42364	NN	denotes	t
T8042	42365-42366	SYM	denotes	=
T8041	42367-42368	NN	denotes	t
T8043	42368-42370	,	denotes	,
T8044	42370-42373	NN	denotes	x 0
T8045	42374-42376	VBZ	denotes	is
T8046	42377-42380	DT	denotes	the
T8047	42381-42386	NN	denotes	OD600
T8048	42387-42389	IN	denotes	at
T8049	42390-42391	NN	denotes	t
T8051	42392-42393	SYM	denotes	=
T8050	42394-42395	CD	denotes	0
T8052	42396-42399	CC	denotes	and
T8053	42400-42401	NN	denotes	t
T8054	42402-42404	VBZ	denotes	is
T8055	42405-42408	DT	denotes	the
T8056	42409-42413	NN	denotes	time
T8057	42414-42421	IN	denotes	between
T8058	42422-42425	NN	denotes	x 0
T8059	42426-42429	CC	denotes	and
T8060	42430-42433	NN	denotes	x t
T8061	42433-42434	.	denotes	.
T8062	42434-42586	sentence	denotes	Assays were performed with three independent clones and three samples of each clone, the results were averaged, and the standard deviations calculated.
T8063	42435-42441	NNS	denotes	Assays
T8065	42442-42446	VBD	denotes	were
T8064	42447-42456	VBN	denotes	performed
T8066	42457-42461	IN	denotes	with
T8067	42462-42467	CD	denotes	three
T8069	42468-42479	JJ	denotes	independent
T8068	42480-42486	NNS	denotes	clones
T8070	42487-42490	CC	denotes	and
T8071	42491-42496	CD	denotes	three
T8072	42497-42504	NNS	denotes	samples
T8073	42505-42507	IN	denotes	of
T8074	42508-42512	DT	denotes	each
T8075	42513-42518	NN	denotes	clone
T8076	42518-42520	,	denotes	,
T8077	42520-42523	DT	denotes	the
T8078	42524-42531	NNS	denotes	results
T8080	42532-42536	VBD	denotes	were
T8079	42537-42545	VBN	denotes	averaged
T8081	42545-42547	,	denotes	,
T8082	42547-42550	CC	denotes	and
T8083	42551-42554	DT	denotes	the
T8085	42555-42563	JJ	denotes	standard
T8084	42564-42574	NNS	denotes	deviations
T8086	42575-42585	VBN	denotes	calculated
T8087	42585-42586	.	denotes	.
T8119	42588-42592	NN	denotes	Site
T8121	42592-42593	HYPH	denotes	-
T8120	42593-42601	VBN	denotes	directed
T8123	42602-42605	CC	denotes	and
T8124	42606-42614	NN	denotes	deletion
T8122	42615-42626	NN	denotes	mutagenesis
T8125	42627-42630	CC	denotes	and
T8126	42631-42638	NN	denotes	plasmid
T8127	42639-42651	NN	denotes	construction
T8128	42651-42652	.	denotes	.
T8129	42652-42772	sentence	denotes	Deletion mutagenesis was carried out with pairs of primers against domains of interest described in the figure legends.
T8130	42653-42661	NN	denotes	Deletion
T8131	42662-42673	NN	denotes	mutagenesis
T8133	42674-42677	VBD	denotes	was
T8132	42678-42685	VBN	denotes	carried
T8134	42686-42689	RP	denotes	out
T8135	42690-42694	IN	denotes	with
T8136	42695-42700	NNS	denotes	pairs
T8137	42701-42703	IN	denotes	of
T8138	42704-42711	NNS	denotes	primers
T8139	42712-42719	IN	denotes	against
T8140	42720-42727	NNS	denotes	domains
T8141	42728-42730	IN	denotes	of
T8142	42731-42739	NN	denotes	interest
T8143	42740-42749	VBN	denotes	described
T8144	42750-42752	IN	denotes	in
T8145	42753-42756	DT	denotes	the
T8147	42757-42763	NN	denotes	figure
T8146	42764-42771	NNS	denotes	legends
T8148	42771-42772	.	denotes	.
T8149	42772-42882	sentence	denotes	PCR products were subcloned into pGEX-KG [64], HybriZAP pBD-GAL4 vector (Stratagene), and pBUTE [62] vectors.
T8150	42773-42776	NN	denotes	PCR
T8151	42777-42785	NNS	denotes	products
T8153	42786-42790	VBD	denotes	were
T8152	42791-42800	VBN	denotes	subcloned
T8154	42801-42805	IN	denotes	into
T8155	42806-42810	NN	denotes	pGEX
T8157	42810-42811	HYPH	denotes	-
T8156	42811-42813	NN	denotes	KG
T8158	42814-42815	-LRB-	denotes	[
T8159	42815-42817	CD	denotes	64
T8160	42817-42818	-RRB-	denotes	]
T8161	42818-42820	,	denotes	,
T8162	42820-42828	NN	denotes	HybriZAP
T8164	42829-42832	NN	denotes	pBD
T8166	42832-42833	HYPH	denotes	-
T8165	42833-42837	NN	denotes	GAL4
T8163	42838-42844	NN	denotes	vector
T8167	42845-42846	-LRB-	denotes	(
T8168	42846-42856	NNP	denotes	Stratagene
T8169	42856-42857	-RRB-	denotes	)
T8170	42857-42859	,	denotes	,
T8171	42859-42862	CC	denotes	and
T8172	42863-42868	NN	denotes	pBUTE
T8174	42869-42870	-LRB-	denotes	[
T8175	42870-42872	CD	denotes	62
T8176	42872-42873	-RRB-	denotes	]
T8173	42874-42881	NNS	denotes	vectors
T8177	42881-42882	.	denotes	.
T8178	42882-42942	sentence	denotes	GST-LDZIP alone comprised residues 447–483 of human RanBP2.
T8179	42883-42886	NN	denotes	GST
T8181	42886-42887	HYPH	denotes	-
T8180	42887-42892	NN	denotes	LDZIP
T8183	42893-42898	RB	denotes	alone
T8182	42899-42908	VBN	denotes	comprised
T8184	42909-42917	NNS	denotes	residues
T8185	42918-42921	CD	denotes	447
T8186	42921-42922	SYM	denotes	–
T8187	42922-42925	CD	denotes	483
T8188	42926-42928	IN	denotes	of
T8189	42929-42934	JJ	denotes	human
T8190	42935-42941	NN	denotes	RanBP2
T8191	42941-42942	.	denotes	.
T8257	42944-42947	NN	denotes	GST
T8259	42948-42952	VB	denotes	pull
T8260	42952-42953	HYPH	denotes	-
T8261	42953-42957	RP	denotes	down
T8262	42958-42961	CC	denotes	and
T8263	42962-42981	NN	denotes	immunoprecipitation
T8258	42982-42988	NNS	denotes	assays
T8264	42988-42989	.	denotes	.
T8265	42989-43122	sentence	denotes	CHAPS-solubilized retinal extracts, expression, and purification of GST-fused constructs were prepared as previously described [65].
T8266	42990-42995	NN	denotes	CHAPS
T8268	42995-42996	HYPH	denotes	-
T8267	42996-43007	VBN	denotes	solubilized
T8270	43008-43015	JJ	denotes	retinal
T8269	43016-43024	NNS	denotes	extracts
T8272	43024-43026	,	denotes	,
T8273	43026-43036	NN	denotes	expression
T8274	43036-43038	,	denotes	,
T8275	43038-43041	CC	denotes	and
T8276	43042-43054	NN	denotes	purification
T8277	43055-43057	IN	denotes	of
T8278	43058-43061	NN	denotes	GST
T8280	43061-43062	HYPH	denotes	-
T8279	43062-43067	VBN	denotes	fused
T8281	43068-43078	NNS	denotes	constructs
T8282	43079-43083	VBD	denotes	were
T8271	43084-43092	VBN	denotes	prepared
T8283	43093-43095	IN	denotes	as
T8285	43096-43106	RB	denotes	previously
T8284	43107-43116	VBN	denotes	described
T8286	43117-43118	-LRB-	denotes	[
T8287	43118-43120	CD	denotes	65
T8288	43120-43121	-RRB-	denotes	]
T8289	43121-43122	.	denotes	.
T8290	43122-43204	sentence	denotes	GST pull-down assays were carried out with 0.5–2.2 μM of GST-fused proteins [65].
T8291	43123-43126	NN	denotes	GST
T8293	43127-43131	VB	denotes	pull
T8294	43131-43132	HYPH	denotes	-
T8295	43132-43136	RP	denotes	down
T8292	43137-43143	NNS	denotes	assays
T8297	43144-43148	VBD	denotes	were
T8296	43149-43156	VBN	denotes	carried
T8298	43157-43160	RP	denotes	out
T8299	43161-43165	IN	denotes	with
T8300	43166-43169	CD	denotes	0.5
T8302	43169-43170	SYM	denotes	–
T8301	43170-43173	CD	denotes	2.2
T8303	43174-43176	NNS	denotes	μM
T8304	43177-43179	IN	denotes	of
T8305	43180-43183	NN	denotes	GST
T8307	43183-43184	HYPH	denotes	-
T8306	43184-43189	VBN	denotes	fused
T8308	43190-43198	NN	denotes	proteins
T8309	43199-43200	-LRB-	denotes	[
T8310	43200-43202	CD	denotes	65
T8311	43202-43203	-RRB-	denotes	]
T8312	43203-43204	.	denotes	.
T8313	43204-43325	sentence	denotes	Co-precipitates were resolved on SDS-PAGE and analyzed by Western blot with antibodies described in the Results section.
T8314	43205-43220	NNS	denotes	Co-precipitates
T8316	43221-43225	VBD	denotes	were
T8315	43226-43234	VBN	denotes	resolved
T8317	43235-43237	IN	denotes	on
T8318	43238-43241	NN	denotes	SDS
T8320	43241-43242	HYPH	denotes	-
T8319	43242-43246	NN	denotes	PAGE
T8321	43247-43250	CC	denotes	and
T8322	43251-43259	VBN	denotes	analyzed
T8323	43260-43262	IN	denotes	by
T8324	43263-43270	NNP	denotes	Western
T8325	43271-43275	NN	denotes	blot
T8326	43276-43280	IN	denotes	with
T8327	43281-43291	NNS	denotes	antibodies
T8328	43292-43301	VBN	denotes	described
T8329	43302-43304	IN	denotes	in
T8330	43305-43308	DT	denotes	the
T8332	43309-43316	NNS	denotes	Results
T8331	43317-43324	NN	denotes	section
T8333	43324-43325	.	denotes	.
T8334	43325-43497	sentence	denotes	Unfolded and partially denatured Cox11 were generated by incubating recombinant and native Cox11 overnight with 5.6 and 7 M guanidine hydrochloride and urea, respectively.
T8335	43326-43334	VBN	denotes	Unfolded
T8337	43335-43338	CC	denotes	and
T8338	43339-43348	RB	denotes	partially
T8339	43349-43358	VBN	denotes	denatured
T8336	43359-43364	NN	denotes	Cox11
T8341	43365-43369	VBD	denotes	were
T8340	43370-43379	VBN	denotes	generated
T8342	43380-43382	IN	denotes	by
T8343	43383-43393	VBG	denotes	incubating
T8344	43394-43405	JJ	denotes	recombinant
T8346	43406-43409	CC	denotes	and
T8347	43410-43416	JJ	denotes	native
T8345	43417-43422	NN	denotes	Cox11
T8348	43423-43432	RB	denotes	overnight
T8349	43433-43437	IN	denotes	with
T8350	43438-43441	CD	denotes	5.6
T8352	43442-43445	CC	denotes	and
T8353	43446-43447	CD	denotes	7
T8351	43448-43449	NNS	denotes	M
T8354	43450-43459	NN	denotes	guanidine
T8355	43460-43473	NN	denotes	hydrochloride
T8356	43474-43477	CC	denotes	and
T8357	43478-43482	NN	denotes	urea
T8358	43482-43484	,	denotes	,
T8359	43484-43496	RB	denotes	respectively
T8360	43496-43497	.	denotes	.
T8361	43497-43588	sentence	denotes	The Cox11 conformers were then diluted ~20-fold in CHAPS-binding buffer containing GST-LD.
T8362	43498-43501	DT	denotes	The
T8364	43502-43507	NN	denotes	Cox11
T8363	43508-43518	NNS	denotes	conformers
T8366	43519-43523	VBD	denotes	were
T8367	43524-43528	RB	denotes	then
T8365	43529-43536	VBN	denotes	diluted
T8368	43537-43538	SYM	denotes	~
T8369	43538-43545	RB	denotes	20-fold
T8370	43546-43548	IN	denotes	in
T8371	43549-43554	NN	denotes	CHAPS
T8373	43554-43555	HYPH	denotes	-
T8372	43555-43562	NN	denotes	binding
T8374	43563-43569	NN	denotes	buffer
T8375	43570-43580	VBG	denotes	containing
T8376	43581-43584	NN	denotes	GST
T8378	43584-43585	HYPH	denotes	-
T8377	43585-43587	NN	denotes	LD
T8379	43587-43588	.	denotes	.
T8380	43588-43738	sentence	denotes	Immunoprecipitation assays with 5 μg of antibody and Western blots (~ 200–400 ng/ml of antibody) were performed exactly as described previously [13].
T8381	43589-43608	NN	denotes	Immunoprecipitation
T8382	43609-43615	NNS	denotes	assays
T8384	43616-43620	IN	denotes	with
T8385	43621-43622	CD	denotes	5
T8386	43623-43625	NNS	denotes	μg
T8387	43626-43628	IN	denotes	of
T8388	43629-43637	NN	denotes	antibody
T8389	43638-43641	CC	denotes	and
T8390	43642-43649	NNP	denotes	Western
T8391	43650-43655	NNS	denotes	blots
T8392	43656-43657	-LRB-	denotes	(
T8394	43657-43658	SYM	denotes	~
T8396	43659-43662	CD	denotes	200
T8397	43662-43663	SYM	denotes	–
T8395	43663-43666	CD	denotes	400
T8393	43667-43669	NN	denotes	ng
T8398	43669-43670	SYM	denotes	/
T8399	43670-43672	NN	denotes	ml
T8400	43673-43675	IN	denotes	of
T8401	43676-43684	NN	denotes	antibody
T8402	43684-43685	-RRB-	denotes	)
T8403	43686-43690	VBD	denotes	were
T8383	43691-43700	VBN	denotes	performed
T8404	43701-43708	RB	denotes	exactly
T8406	43709-43711	IN	denotes	as
T8405	43712-43721	VBN	denotes	described
T8407	43722-43732	RB	denotes	previously
T8408	43733-43734	-LRB-	denotes	[
T8409	43734-43736	CD	denotes	13
T8410	43736-43737	-RRB-	denotes	]
T8411	43737-43738	.	denotes	.
T8483	43740-43750	NN	denotes	Hexokinase
T8485	43751-43752	CD	denotes	I
T8484	43753-43758	NN	denotes	assay
T8486	43758-43759	.	denotes	.
T8487	43759-44006	sentence	denotes	Hexokinase I activity was determined spectrophotometrically at 25 °C by the method of coupling the glucose-6-phosphate production via glucose-6-phosphate dehydrogenase with the change in the absorbance of NADPH at 340 nm and as described by [66].
T8488	43760-43770	NN	denotes	Hexokinase
T8490	43771-43772	CD	denotes	I
T8489	43773-43781	NN	denotes	activity
T8492	43782-43785	VBD	denotes	was
T8491	43786-43796	VBN	denotes	determined
T8493	43797-43819	RB	denotes	spectrophotometrically
T8494	43820-43822	IN	denotes	at
T8495	43823-43825	CD	denotes	25
T8496	43826-43828	NN	denotes	°C
T8497	43829-43831	IN	denotes	by
T8498	43832-43835	DT	denotes	the
T8499	43836-43842	NN	denotes	method
T8500	43843-43845	IN	denotes	of
T8501	43846-43854	VBG	denotes	coupling
T8502	43855-43858	DT	denotes	the
T8504	43859-43866	NN	denotes	glucose
T8506	43866-43867	HYPH	denotes	-
T8507	43867-43868	CD	denotes	6
T8508	43868-43869	HYPH	denotes	-
T8505	43869-43878	NN	denotes	phosphate
T8503	43879-43889	NN	denotes	production
T8509	43890-43893	IN	denotes	via
T8510	43894-43901	NN	denotes	glucose
T8512	43901-43902	HYPH	denotes	-
T8513	43902-43903	CD	denotes	6
T8514	43903-43904	HYPH	denotes	-
T8511	43904-43913	NN	denotes	phosphate
T8515	43914-43927	NN	denotes	dehydrogenase
T8516	43928-43932	IN	denotes	with
T8517	43933-43936	DT	denotes	the
T8518	43937-43943	NN	denotes	change
T8519	43944-43946	IN	denotes	in
T8520	43947-43950	DT	denotes	the
T8521	43951-43961	NN	denotes	absorbance
T8522	43962-43964	IN	denotes	of
T8523	43965-43970	NN	denotes	NADPH
T8524	43971-43973	IN	denotes	at
T8525	43974-43977	CD	denotes	340
T8526	43978-43980	NN	denotes	nm
T8527	43981-43984	CC	denotes	and
T8528	43985-43987	IN	denotes	as
T8529	43988-43997	VBN	denotes	described
T8530	43998-44000	IN	denotes	by
T8531	44001-44002	-LRB-	denotes	[
T8532	44002-44004	CD	denotes	66
T8533	44004-44005	-RRB-	denotes	]
T8534	44005-44006	.	denotes	.
T8535	44006-44292	sentence	denotes	The reaction was started by the addition of purified brain hexokinase I (0.24 μg) (gift from J. Wilson) to 1.0 ml of reaction mixture containing 0.05 M Tris-HCl (pH 8.5), 7.4 mM MgCl2, 6.6 mM ATP, 0.65 mM NADP, 11.1 mM monothioglycerol, and 1 unit of glucose 6-phosphate dehydrogenase.
T8536	44007-44010	DT	denotes	The
T8537	44011-44019	NN	denotes	reaction
T8539	44020-44023	VBD	denotes	was
T8538	44024-44031	VBN	denotes	started
T8540	44032-44034	IN	denotes	by
T8541	44035-44038	DT	denotes	the
T8542	44039-44047	NN	denotes	addition
T8543	44048-44050	IN	denotes	of
T8544	44051-44059	VBN	denotes	purified
T8546	44060-44065	NN	denotes	brain
T8545	44066-44076	NN	denotes	hexokinase
T8547	44077-44078	CD	denotes	I
T8548	44079-44080	-LRB-	denotes	(
T8550	44080-44084	CD	denotes	0.24
T8549	44085-44087	NNS	denotes	μg
T8551	44087-44088	-RRB-	denotes	)
T8552	44089-44090	-LRB-	denotes	(
T8553	44090-44094	NN	denotes	gift
T8554	44095-44099	IN	denotes	from
T8555	44100-44102	NNP	denotes	J.
T8556	44103-44109	NNP	denotes	Wilson
T8557	44109-44110	-RRB-	denotes	)
T8558	44111-44113	IN	denotes	to
T8559	44114-44117	CD	denotes	1.0
T8560	44118-44120	NN	denotes	ml
T8561	44121-44123	IN	denotes	of
T8562	44124-44132	NN	denotes	reaction
T8563	44133-44140	NN	denotes	mixture
T8564	44141-44151	VBG	denotes	containing
T8565	44152-44156	CD	denotes	0.05
T8566	44157-44158	NNS	denotes	M
T8568	44159-44163	NN	denotes	Tris
T8569	44163-44164	HYPH	denotes	-
T8567	44164-44167	NN	denotes	HCl
T8570	44168-44169	-LRB-	denotes	(
T8571	44169-44171	NN	denotes	pH
T8572	44172-44175	CD	denotes	8.5
T8573	44175-44176	-RRB-	denotes	)
T8574	44176-44178	,	denotes	,
T8575	44178-44181	CD	denotes	7.4
T8576	44182-44184	NNS	denotes	mM
T8577	44185-44190	NN	denotes	MgCl2
T8578	44190-44192	,	denotes	,
T8579	44192-44195	CD	denotes	6.6
T8580	44196-44198	NNS	denotes	mM
T8581	44199-44202	NN	denotes	ATP
T8582	44202-44204	,	denotes	,
T8583	44204-44208	CD	denotes	0.65
T8584	44209-44211	NNS	denotes	mM
T8585	44212-44216	NN	denotes	NADP
T8586	44216-44218	,	denotes	,
T8587	44218-44222	CD	denotes	11.1
T8588	44223-44225	NNS	denotes	mM
T8589	44226-44242	NN	denotes	monothioglycerol
T8590	44242-44244	,	denotes	,
T8591	44244-44247	CC	denotes	and
T8592	44248-44249	CD	denotes	1
T8593	44250-44254	NN	denotes	unit
T8594	44255-44257	IN	denotes	of
T8595	44258-44265	NN	denotes	glucose
T8597	44266-44267	CD	denotes	6
T8598	44267-44268	HYPH	denotes	-
T8596	44268-44277	NN	denotes	phosphate
T8599	44278-44291	NN	denotes	dehydrogenase
T8600	44291-44292	.	denotes	.
T8601	44292-44485	sentence	denotes	In the case of hexokinase activity measured in the presence of Cox11 and LD, the purified HKI was incubated with the recombinant proteins for 15 min at 4 °C before measurement of the activity.
T8602	44293-44295	IN	denotes	In
T8604	44296-44299	DT	denotes	the
T8605	44300-44304	NN	denotes	case
T8606	44305-44307	IN	denotes	of
T8607	44308-44318	NN	denotes	hexokinase
T8608	44319-44327	NN	denotes	activity
T8609	44328-44336	VBN	denotes	measured
T8610	44337-44339	IN	denotes	in
T8611	44340-44343	DT	denotes	the
T8612	44344-44352	NN	denotes	presence
T8613	44353-44355	IN	denotes	of
T8614	44356-44361	NN	denotes	Cox11
T8615	44362-44365	CC	denotes	and
T8616	44366-44368	NN	denotes	LD
T8617	44368-44370	,	denotes	,
T8618	44370-44373	DT	denotes	the
T8620	44374-44382	VBN	denotes	purified
T8619	44383-44386	NN	denotes	HKI
T8621	44387-44390	VBD	denotes	was
T8603	44391-44400	VBN	denotes	incubated
T8622	44401-44405	IN	denotes	with
T8623	44406-44409	DT	denotes	the
T8625	44410-44421	JJ	denotes	recombinant
T8624	44422-44430	NN	denotes	proteins
T8626	44431-44434	IN	denotes	for
T8627	44435-44437	CD	denotes	15
T8628	44438-44441	NNS	denotes	min
T8629	44442-44444	IN	denotes	at
T8630	44445-44446	CD	denotes	4
T8631	44447-44449	NN	denotes	°C
T8632	44450-44456	IN	denotes	before
T8633	44457-44468	NN	denotes	measurement
T8634	44469-44471	IN	denotes	of
T8635	44472-44475	DT	denotes	the
T8636	44476-44484	NN	denotes	activity
T8637	44484-44485	.	denotes	.
T8638	44485-44582	sentence	denotes	The data were fitted directly into the Michaelis-Menten equation using SIGMAPLOT (SPSS Science).
T8639	44486-44489	DT	denotes	The
T8640	44490-44494	NNS	denotes	data
T8642	44495-44499	VBD	denotes	were
T8641	44500-44506	VBN	denotes	fitted
T8643	44507-44515	RB	denotes	directly
T8644	44516-44520	IN	denotes	into
T8645	44521-44524	DT	denotes	the
T8647	44525-44534	NNP	denotes	Michaelis
T8649	44534-44535	HYPH	denotes	-
T8648	44535-44541	NNP	denotes	Menten
T8646	44542-44550	NN	denotes	equation
T8650	44551-44556	VBG	denotes	using
T8651	44557-44566	NNP	denotes	SIGMAPLOT
T8652	44567-44568	-LRB-	denotes	(
T8654	44568-44572	NNP	denotes	SPSS
T8653	44573-44580	NNP	denotes	Science
T8655	44580-44581	-RRB-	denotes	)
T8656	44581-44582	.	denotes	.
T8700	44584-44594	NNS	denotes	Antibodies
T8701	44594-44595	.	denotes	.
T8702	44595-44775	sentence	denotes	Rabbit antisera were raised against the recombinant murine Cox11 (residues 40–275) and affinity-purified under non-denaturing conditions (Stereogene) as previously described [13].
T8703	44596-44602	NN	denotes	Rabbit
T8704	44603-44611	NNS	denotes	antisera
T8706	44612-44616	VBD	denotes	were
T8705	44617-44623	VBN	denotes	raised
T8707	44624-44631	IN	denotes	against
T8708	44632-44635	DT	denotes	the
T8710	44636-44647	JJ	denotes	recombinant
T8711	44648-44654	JJ	denotes	murine
T8709	44655-44660	NN	denotes	Cox11
T8712	44661-44662	-LRB-	denotes	(
T8714	44662-44670	NNS	denotes	residues
T8713	44671-44673	CD	denotes	40
T8715	44673-44674	SYM	denotes	–
T8716	44674-44677	CD	denotes	275
T8717	44677-44678	-RRB-	denotes	)
T8718	44679-44682	CC	denotes	and
T8719	44683-44691	NN	denotes	affinity
T8721	44691-44692	HYPH	denotes	-
T8720	44692-44700	VBN	denotes	purified
T8722	44701-44706	IN	denotes	under
T8723	44707-44721	NN	denotes	non-denaturing
T8724	44722-44732	NNS	denotes	conditions
T8725	44733-44734	-LRB-	denotes	(
T8726	44734-44744	NNP	denotes	Stereogene
T8727	44744-44745	-RRB-	denotes	)
T8728	44746-44748	IN	denotes	as
T8730	44749-44759	RB	denotes	previously
T8729	44760-44769	VBN	denotes	described
T8731	44770-44771	-LRB-	denotes	[
T8732	44771-44773	CD	denotes	13
T8733	44773-44774	-RRB-	denotes	]
T8734	44774-44775	.	denotes	.
T8735	44775-44837	sentence	denotes	Cox11 antibodies were used at 200 ng/ml for Western analysis.
T8736	44776-44781	NN	denotes	Cox11
T8737	44782-44792	NNS	denotes	antibodies
T8739	44793-44797	VBD	denotes	were
T8738	44798-44802	VBN	denotes	used
T8740	44803-44805	IN	denotes	at
T8741	44806-44809	CD	denotes	200
T8742	44810-44812	NNS	denotes	ng
T8743	44812-44813	SYM	denotes	/
T8744	44813-44815	NN	denotes	ml
T8745	44816-44819	IN	denotes	for
T8746	44820-44827	NNP	denotes	Western
T8747	44828-44836	NN	denotes	analysis
T8748	44836-44837	.	denotes	.
T8749	44837-45023	sentence	denotes	The monoclonal antibody Hsp70 was from Affinity Bioreagents (Golden, Colorado, United States) and antibodies against the KBD (JX2) and ZnF domains of RanBP2 have been described [13,16].
T8750	44838-44841	DT	denotes	The
T8752	44842-44852	JJ	denotes	monoclonal
T8753	44853-44861	NN	denotes	antibody
T8751	44862-44867	NN	denotes	Hsp70
T8754	44868-44871	VBD	denotes	was
T8755	44872-44876	IN	denotes	from
T8756	44877-44885	NNP	denotes	Affinity
T8757	44886-44897	NNPS	denotes	Bioreagents
T8758	44898-44899	-LRB-	denotes	(
T8759	44899-44905	NNP	denotes	Golden
T8760	44905-44907	,	denotes	,
T8761	44907-44915	NNP	denotes	Colorado
T8762	44915-44917	,	denotes	,
T8763	44917-44923	NNP	denotes	United
T8764	44924-44930	NNP	denotes	States
T8765	44930-44931	-RRB-	denotes	)
T8766	44932-44935	CC	denotes	and
T8767	44936-44946	NNS	denotes	antibodies
T8769	44947-44954	IN	denotes	against
T8770	44955-44958	DT	denotes	the
T8772	44959-44962	NN	denotes	KBD
T8773	44963-44964	-LRB-	denotes	(
T8774	44964-44967	NN	denotes	JX2
T8775	44967-44968	-RRB-	denotes	)
T8776	44969-44972	CC	denotes	and
T8777	44973-44976	NN	denotes	ZnF
T8771	44977-44984	NNS	denotes	domains
T8778	44985-44987	IN	denotes	of
T8779	44988-44994	NN	denotes	RanBP2
T8780	44995-44999	VBP	denotes	have
T8781	45000-45004	VBN	denotes	been
T8768	45005-45014	VBN	denotes	described
T8782	45015-45016	-LRB-	denotes	[
T8784	45016-45018	CD	denotes	13
T8785	45018-45019	,	denotes	,
T8783	45019-45021	CD	denotes	16
T8786	45021-45022	-RRB-	denotes	]
T8787	45022-45023	.	denotes	.
T8788	45023-45109	sentence	denotes	Polyclonal and monoclonal antibodies against Hexokinase I were provided by J. Wilson.
T8789	45024-45034	JJ	denotes	Polyclonal
T8791	45035-45038	CC	denotes	and
T8792	45039-45049	JJ	denotes	monoclonal
T8790	45050-45060	NNS	denotes	antibodies
T8794	45061-45068	IN	denotes	against
T8795	45069-45079	NN	denotes	Hexokinase
T8796	45080-45081	PRP	denotes	I
T8797	45082-45086	VBD	denotes	were
T8793	45087-45095	VBN	denotes	provided
T8798	45096-45098	IN	denotes	by
T8799	45099-45101	NNP	denotes	J.
T8800	45102-45108	NNP	denotes	Wilson
T8801	45108-45109	.	denotes	.
T8802	45109-45188	sentence	denotes	The mAb414 was purchased from Abcam (Cambridge, Massachusetts, United States).
T8803	45110-45113	DT	denotes	The
T8804	45114-45120	NN	denotes	mAb414
T8806	45121-45124	VBD	denotes	was
T8805	45125-45134	VBN	denotes	purchased
T8807	45135-45139	IN	denotes	from
T8808	45140-45145	NNP	denotes	Abcam
T8809	45146-45147	-LRB-	denotes	(
T8810	45147-45156	NNP	denotes	Cambridge
T8811	45156-45158	,	denotes	,
T8812	45158-45171	NNP	denotes	Massachusetts
T8813	45171-45173	,	denotes	,
T8814	45173-45179	NNP	denotes	United
T8815	45180-45186	NNP	denotes	States
T8816	45186-45187	-RRB-	denotes	)
T8817	45187-45188	.	denotes	.
T8883	45190-45209	NN	denotes	Immunocytochemistry
T8884	45210-45213	CC	denotes	and
T8885	45214-45224	NN	denotes	microscopy
T8886	45224-45225	.	denotes	.
T8887	45225-45356	sentence	denotes	Retina dissections, radial cryosections (~6 μm), and immunohistochemistry procedures were carried out as described elsewhere [19].
T8888	45226-45232	NN	denotes	Retina
T8889	45233-45244	NNS	denotes	dissections
T8891	45244-45246	,	denotes	,
T8892	45246-45252	JJ	denotes	radial
T8893	45253-45265	NNS	denotes	cryosections
T8894	45266-45267	-LRB-	denotes	(
T8896	45267-45268	SYM	denotes	~
T8897	45268-45269	CD	denotes	6
T8895	45270-45272	NNS	denotes	μm
T8898	45272-45273	-RRB-	denotes	)
T8899	45273-45275	,	denotes	,
T8900	45275-45278	CC	denotes	and
T8901	45279-45299	NN	denotes	immunohistochemistry
T8902	45300-45310	NNS	denotes	procedures
T8903	45311-45315	VBD	denotes	were
T8890	45316-45323	VBN	denotes	carried
T8904	45324-45327	RP	denotes	out
T8905	45328-45330	IN	denotes	as
T8906	45331-45340	VBN	denotes	described
T8907	45341-45350	RB	denotes	elsewhere
T8908	45351-45352	-LRB-	denotes	[
T8909	45352-45354	CD	denotes	19
T8910	45354-45355	-RRB-	denotes	]
T8911	45355-45356	.	denotes	.
T8912	45356-45501	sentence	denotes	Brains from 3- to 5-mo-old C57Bl/6 mice were fixed overnight in 2% paraformaldehyde, infused with 30% sucrose, and processed for cryosectioning.
T8913	45357-45363	NNS	denotes	Brains
T8915	45364-45368	IN	denotes	from
T8916	45369-45370	CD	denotes	3
T8918	45370-45371	HYPH	denotes	-
T8919	45372-45374	IN	denotes	to
T8917	45375-45376	CD	denotes	5
T8921	45376-45377	HYPH	denotes	-
T8920	45377-45379	NN	denotes	mo
T8923	45379-45380	HYPH	denotes	-
T8922	45380-45383	JJ	denotes	old
T8925	45384-45389	NN	denotes	C57Bl
T8926	45389-45390	HYPH	denotes	/
T8927	45390-45391	CD	denotes	6
T8924	45392-45396	NNS	denotes	mice
T8928	45397-45401	VBD	denotes	were
T8914	45402-45407	VBN	denotes	fixed
T8929	45408-45417	RB	denotes	overnight
T8930	45418-45420	IN	denotes	in
T8931	45421-45422	CD	denotes	2
T8932	45422-45423	NN	denotes	%
T8933	45424-45440	NN	denotes	paraformaldehyde
T8934	45440-45442	,	denotes	,
T8935	45442-45449	VBN	denotes	infused
T8936	45450-45454	IN	denotes	with
T8937	45455-45457	CD	denotes	30
T8938	45457-45458	NN	denotes	%
T8939	45459-45466	NN	denotes	sucrose
T8940	45466-45468	,	denotes	,
T8941	45468-45471	CC	denotes	and
T8942	45472-45481	VBN	denotes	processed
T8943	45482-45485	IN	denotes	for
T8944	45486-45500	VBG	denotes	cryosectioning
T8945	45500-45501	.	denotes	.
T8946	45501-45579	sentence	denotes	Primary brain neurons and glial cells were prepared from the cerebral cortex.
T8947	45502-45509	JJ	denotes	Primary
T8949	45510-45515	NN	denotes	brain
T8948	45516-45523	NNS	denotes	neurons
T8951	45524-45527	CC	denotes	and
T8952	45528-45533	JJ	denotes	glial
T8953	45534-45539	NNS	denotes	cells
T8954	45540-45544	VBD	denotes	were
T8950	45545-45553	VBN	denotes	prepared
T8955	45554-45558	IN	denotes	from
T8956	45559-45562	DT	denotes	the
T8958	45563-45571	JJ	denotes	cerebral
T8957	45572-45578	NN	denotes	cortex
T8959	45578-45579	.	denotes	.
T8960	45579-45651	sentence	denotes	This was macerated in the Hank's Balanced Salt solution and triturated.
T8961	45580-45584	DT	denotes	This
T8963	45585-45588	VBD	denotes	was
T8962	45589-45598	VBN	denotes	macerated
T8964	45599-45601	IN	denotes	in
T8965	45602-45605	DT	denotes	the
T8967	45606-45610	NNP	denotes	Hank
T8969	45610-45612	POS	denotes	's
T8970	45613-45621	NNP	denotes	Balanced
T8968	45622-45626	NNP	denotes	Salt
T8966	45627-45635	NN	denotes	solution
T8971	45636-45639	CC	denotes	and
T8972	45640-45650	VBN	denotes	triturated
T8973	45650-45651	.	denotes	.
T8974	45651-45888	sentence	denotes	Primary cells were cultured in DMEM (GIBCO, San Diego, California, United States) and collagen-coated 35-mm glass bottom culture dishes (MatTek Corporation, Ashland, Maine) at 5% CO2/37 °C for ~2 d and processed for immunocytochemistry.
T8975	45652-45659	JJ	denotes	Primary
T8976	45660-45665	NNS	denotes	cells
T8978	45666-45670	VBD	denotes	were
T8977	45671-45679	VBN	denotes	cultured
T8979	45680-45682	IN	denotes	in
T8980	45683-45687	NN	denotes	DMEM
T8981	45688-45689	-LRB-	denotes	(
T8982	45689-45694	NNP	denotes	GIBCO
T8983	45694-45696	,	denotes	,
T8984	45696-45699	NNP	denotes	San
T8985	45700-45705	NNP	denotes	Diego
T8986	45705-45707	,	denotes	,
T8987	45707-45717	NNP	denotes	California
T8988	45717-45719	,	denotes	,
T8989	45719-45725	NNP	denotes	United
T8990	45726-45732	NNP	denotes	States
T8991	45732-45733	-RRB-	denotes	)
T8992	45734-45737	CC	denotes	and
T8993	45738-45746	NN	denotes	collagen
T8995	45746-45747	HYPH	denotes	-
T8994	45747-45753	VBN	denotes	coated
T8997	45754-45756	CD	denotes	35
T8999	45756-45757	HYPH	denotes	-
T8998	45757-45759	NN	denotes	mm
T9000	45760-45765	NN	denotes	glass
T9001	45766-45772	NN	denotes	bottom
T9002	45773-45780	NN	denotes	culture
T8996	45781-45787	NNS	denotes	dishes
T9003	45788-45789	-LRB-	denotes	(
T9005	45789-45795	NNP	denotes	MatTek
T9004	45796-45807	NNP	denotes	Corporation
T9006	45807-45809	,	denotes	,
T9007	45809-45816	NNP	denotes	Ashland
T9008	45816-45818	,	denotes	,
T9009	45818-45823	NNP	denotes	Maine
T9010	45823-45824	-RRB-	denotes	)
T9011	45825-45827	IN	denotes	at
T9012	45828-45829	CD	denotes	5
T9013	45829-45830	NN	denotes	%
T9014	45831-45834	NN	denotes	CO2
T9015	45834-45835	HYPH	denotes	/
T9016	45835-45837	CD	denotes	37
T9017	45838-45840	NN	denotes	°C
T9018	45841-45844	IN	denotes	for
T9019	45845-45846	SYM	denotes	~
T9020	45846-45847	CD	denotes	2
T9021	45848-45849	NNS	denotes	d
T9022	45850-45853	CC	denotes	and
T9023	45854-45863	VBN	denotes	processed
T9024	45864-45867	IN	denotes	for
T9025	45868-45887	NN	denotes	immunocytochemistry
T9026	45887-45888	.	denotes	.
T9027	45888-45950	sentence	denotes	Primary antibodies were used at concentrations ~2.5–10 μg/ml.
T9028	45889-45896	JJ	denotes	Primary
T9029	45897-45907	NNS	denotes	antibodies
T9031	45908-45912	VBD	denotes	were
T9030	45913-45917	VBN	denotes	used
T9032	45918-45920	IN	denotes	at
T9033	45921-45935	NNS	denotes	concentrations
T9034	45936-45937	SYM	denotes	~
T9036	45937-45940	CD	denotes	2.5
T9037	45940-45941	SYM	denotes	–
T9035	45941-45943	CD	denotes	10
T9038	45944-45946	NN	denotes	μg
T9039	45946-45947	SYM	denotes	/
T9040	45947-45949	NN	denotes	ml
T9041	45949-45950	.	denotes	.
T9042	45950-46079	sentence	denotes	Alexa 488- and Alexa 594-conjugated secondary antibodies (2.5 μg/ml) (Molecular Probes) were used for visualization of proteins.
T9043	45951-45956	NNP	denotes	Alexa
T9045	45957-45960	CD	denotes	488
T9046	45960-45961	HYPH	denotes	-
T9047	45962-45965	CC	denotes	and
T9048	45966-45971	NNP	denotes	Alexa
T9049	45972-45975	JJ	denotes	594
T9050	45975-45976	HYPH	denotes	-
T9044	45976-45986	JJ	denotes	conjugated
T9052	45987-45996	JJ	denotes	secondary
T9051	45997-46007	NNS	denotes	antibodies
T9054	46008-46009	-LRB-	denotes	(
T9056	46009-46012	CD	denotes	2.5
T9055	46013-46015	NNS	denotes	μg
T9057	46015-46016	SYM	denotes	/
T9058	46016-46018	NN	denotes	ml
T9059	46018-46019	-RRB-	denotes	)
T9060	46020-46021	-LRB-	denotes	(
T9062	46021-46030	NNP	denotes	Molecular
T9061	46031-46037	NNP	denotes	Probes
T9063	46037-46038	-RRB-	denotes	)
T9064	46039-46043	VBD	denotes	were
T9053	46044-46048	VBN	denotes	used
T9065	46049-46052	IN	denotes	for
T9066	46053-46066	NN	denotes	visualization
T9067	46067-46069	IN	denotes	of
T9068	46070-46078	NN	denotes	proteins
T9069	46078-46079	.	denotes	.
T9070	46079-46174	sentence	denotes	Crossover of fluorescent probes, background, and autofluorescence were found to be negligible.
T9071	46080-46089	NN	denotes	Crossover
T9073	46090-46092	IN	denotes	of
T9074	46093-46104	JJ	denotes	fluorescent
T9075	46105-46111	NNS	denotes	probes
T9076	46111-46113	,	denotes	,
T9077	46113-46123	NN	denotes	background
T9078	46123-46125	,	denotes	,
T9079	46125-46128	CC	denotes	and
T9080	46129-46145	NN	denotes	autofluorescence
T9081	46146-46150	VBD	denotes	were
T9072	46151-46156	VBN	denotes	found
T9082	46157-46159	TO	denotes	to
T9083	46160-46162	VB	denotes	be
T9084	46163-46173	JJ	denotes	negligible
T9085	46173-46174	.	denotes	.
T9086	46174-46412	sentence	denotes	Visualization of specimens and localization of proteins were carried out by wide-field epifluorescence microscopy on Nikon (Tokyo, Japan) E600 upright and TE2000U inverted research microscopes equipped with similar Apochromat objectives.
T9087	46175-46188	NN	denotes	Visualization
T9089	46189-46191	IN	denotes	of
T9090	46192-46201	NNS	denotes	specimens
T9091	46202-46205	CC	denotes	and
T9092	46206-46218	NN	denotes	localization
T9093	46219-46221	IN	denotes	of
T9094	46222-46230	NN	denotes	proteins
T9095	46231-46235	VBD	denotes	were
T9088	46236-46243	VBN	denotes	carried
T9096	46244-46247	RP	denotes	out
T9097	46248-46250	IN	denotes	by
T9098	46251-46255	JJ	denotes	wide
T9100	46255-46256	HYPH	denotes	-
T9099	46256-46261	NN	denotes	field
T9102	46262-46277	NN	denotes	epifluorescence
T9101	46278-46288	NN	denotes	microscopy
T9103	46289-46291	IN	denotes	on
T9104	46292-46297	NNP	denotes	Nikon
T9106	46298-46299	-LRB-	denotes	(
T9107	46299-46304	NNP	denotes	Tokyo
T9108	46304-46306	,	denotes	,
T9109	46306-46311	NNP	denotes	Japan
T9110	46311-46312	-RRB-	denotes	)
T9111	46313-46317	NN	denotes	E600
T9105	46318-46325	NN	denotes	upright
T9112	46326-46329	CC	denotes	and
T9113	46330-46337	NN	denotes	TE2000U
T9115	46338-46346	VBN	denotes	inverted
T9116	46347-46355	NN	denotes	research
T9114	46356-46367	NNS	denotes	microscopes
T9117	46368-46376	VBN	denotes	equipped
T9118	46377-46381	IN	denotes	with
T9119	46382-46389	JJ	denotes	similar
T9121	46390-46400	NN	denotes	Apochromat
T9120	46401-46411	NNS	denotes	objectives
T9122	46411-46412	.	denotes	.
T9123	46412-46619	sentence	denotes	Images with the Nikon E600 were acquired with a SPOT-RT digital camera coupled to the microscope and driven by SPOT Imaging v4.0 software (Diagnostic Instruments, Sterling Heights, Michigan, United States).
T9124	46413-46419	NNS	denotes	Images
T9126	46420-46424	IN	denotes	with
T9127	46425-46428	DT	denotes	the
T9129	46429-46434	NNP	denotes	Nikon
T9128	46435-46439	NN	denotes	E600
T9130	46440-46444	VBD	denotes	were
T9125	46445-46453	VBN	denotes	acquired
T9131	46454-46458	IN	denotes	with
T9132	46459-46460	DT	denotes	a
T9134	46461-46465	NN	denotes	SPOT
T9136	46465-46466	HYPH	denotes	-
T9135	46466-46468	NN	denotes	RT
T9137	46469-46476	JJ	denotes	digital
T9133	46477-46483	NN	denotes	camera
T9138	46484-46491	VBN	denotes	coupled
T9139	46492-46494	IN	denotes	to
T9140	46495-46498	DT	denotes	the
T9141	46499-46509	NN	denotes	microscope
T9142	46510-46513	CC	denotes	and
T9143	46514-46520	VBN	denotes	driven
T9144	46521-46523	IN	denotes	by
T9145	46524-46528	NN	denotes	SPOT
T9147	46529-46536	NN	denotes	Imaging
T9146	46537-46541	NN	denotes	v4.0
T9148	46542-46550	NN	denotes	software
T9149	46551-46552	-LRB-	denotes	(
T9151	46552-46562	NNP	denotes	Diagnostic
T9150	46563-46574	NNPS	denotes	Instruments
T9152	46574-46576	,	denotes	,
T9153	46576-46584	NNP	denotes	Sterling
T9154	46585-46592	NNP	denotes	Heights
T9155	46592-46594	,	denotes	,
T9156	46594-46602	NNP	denotes	Michigan
T9157	46602-46604	,	denotes	,
T9158	46604-46610	NNP	denotes	United
T9159	46611-46617	NNP	denotes	States
T9160	46617-46618	-RRB-	denotes	)
T9161	46618-46619	.	denotes	.
T9162	46619-46731	sentence	denotes	All images were captured at nonsaturating integration levels, 12-bit mono black/white, and then pseudo-colored.
T9163	46620-46623	DT	denotes	All
T9164	46624-46630	NNS	denotes	images
T9166	46631-46635	VBD	denotes	were
T9165	46636-46644	VBN	denotes	captured
T9167	46645-46647	IN	denotes	at
T9168	46648-46661	NN	denotes	nonsaturating
T9170	46662-46673	NN	denotes	integration
T9169	46674-46680	NNS	denotes	levels
T9171	46680-46682	,	denotes	,
T9172	46682-46684	CD	denotes	12
T9174	46684-46685	HYPH	denotes	-
T9173	46685-46688	JJ	denotes	bit
T9176	46689-46693	NN	denotes	mono
T9177	46694-46699	JJ	denotes	black
T9178	46699-46700	HYPH	denotes	/
T9175	46700-46705	JJ	denotes	white
T9179	46705-46707	,	denotes	,
T9180	46707-46710	CC	denotes	and
T9181	46711-46715	RB	denotes	then
T9182	46716-46730	VBN	denotes	pseudo-colored
T9183	46730-46731	.	denotes	.
T9184	46731-47127	sentence	denotes	Protein colocalization analysis was carried out on the Nikon TE2000U microscope equipped with appropriate excitation and emission filter wheels, cube filters, 100 W mercury light source, Nomarski/DIC, and Plan Apochromat optics (100×, 60×, and 40× oil objectives with NA of 1.4, 40 ×LWD, and 20 ×LWD objectives and encoded motorized Z-Stage (Prior Scientific, Rockland, Maryland, United States).
T9185	46732-46739	NN	denotes	Protein
T9186	46740-46754	NN	denotes	colocalization
T9187	46755-46763	NN	denotes	analysis
T9189	46764-46767	VBD	denotes	was
T9188	46768-46775	VBN	denotes	carried
T9190	46776-46779	RP	denotes	out
T9191	46780-46782	IN	denotes	on
T9192	46783-46786	DT	denotes	the
T9194	46787-46792	NNP	denotes	Nikon
T9195	46793-46800	NN	denotes	TE2000U
T9193	46801-46811	NN	denotes	microscope
T9196	46812-46820	VBN	denotes	equipped
T9197	46821-46825	IN	denotes	with
T9198	46826-46837	JJ	denotes	appropriate
T9200	46838-46848	NN	denotes	excitation
T9201	46849-46852	CC	denotes	and
T9202	46853-46861	NN	denotes	emission
T9203	46862-46868	NN	denotes	filter
T9199	46869-46875	NNS	denotes	wheels
T9204	46875-46877	,	denotes	,
T9205	46877-46881	NN	denotes	cube
T9206	46882-46889	NNS	denotes	filters
T9207	46889-46891	,	denotes	,
T9208	46891-46894	CD	denotes	100
T9209	46895-46896	NNP	denotes	W
T9211	46897-46904	NN	denotes	mercury
T9212	46905-46910	NN	denotes	light
T9210	46911-46917	NN	denotes	source
T9213	46917-46919	,	denotes	,
T9214	46919-46927	NNP	denotes	Nomarski
T9216	46927-46928	HYPH	denotes	/
T9215	46928-46931	NNP	denotes	DIC
T9218	46931-46933	,	denotes	,
T9219	46933-46936	CC	denotes	and
T9220	46937-46941	NNP	denotes	Plan
T9221	46942-46952	NNP	denotes	Apochromat
T9217	46953-46959	NNS	denotes	optics
T9222	46960-46961	-LRB-	denotes	(
T9223	46961-46964	CD	denotes	100
T9225	46964-46965	SYM	denotes	×
T9226	46965-46967	,	denotes	,
T9227	46967-46969	CD	denotes	60
T9228	46969-46970	SYM	denotes	×
T9229	46970-46972	,	denotes	,
T9230	46972-46975	CC	denotes	and
T9231	46976-46978	CD	denotes	40
T9232	46978-46979	SYM	denotes	×
T9233	46980-46983	NN	denotes	oil
T9224	46984-46994	NNS	denotes	objectives
T9234	46995-46999	IN	denotes	with
T9235	47000-47002	NN	denotes	NA
T9236	47003-47005	IN	denotes	of
T9237	47006-47009	CD	denotes	1.4
T9238	47009-47011	,	denotes	,
T9239	47011-47013	CD	denotes	40
T9241	47014-47015	SYM	denotes	×
T9240	47015-47018	NN	denotes	LWD
T9243	47018-47020	,	denotes	,
T9244	47020-47023	CC	denotes	and
T9245	47024-47026	CD	denotes	20
T9247	47027-47028	SYM	denotes	×
T9246	47028-47031	NN	denotes	LWD
T9242	47032-47042	NNS	denotes	objectives
T9248	47043-47046	CC	denotes	and
T9249	47047-47054	VBN	denotes	encoded
T9251	47055-47064	VBN	denotes	motorized
T9252	47065-47066	NNP	denotes	Z
T9253	47066-47067	HYPH	denotes	-
T9250	47067-47072	NNP	denotes	Stage
T9254	47073-47074	-LRB-	denotes	(
T9256	47074-47079	NNP	denotes	Prior
T9255	47080-47090	NNP	denotes	Scientific
T9257	47090-47092	,	denotes	,
T9258	47092-47100	NNP	denotes	Rockland
T9259	47100-47102	,	denotes	,
T9260	47102-47110	NNP	denotes	Maryland
T9261	47110-47112	,	denotes	,
T9262	47112-47118	NNP	denotes	United
T9263	47119-47125	NNP	denotes	States
T9264	47125-47126	-RRB-	denotes	)
T9265	47126-47127	.	denotes	.
T9266	47127-47279	sentence	denotes	Images with the inverted Nikon TE2000U microscope were obtained using a CCD camera (CoolSNAP HQ; Roper Scientific, Trenton, New Jersey, United States).
T9267	47128-47134	NNS	denotes	Images
T9269	47135-47139	IN	denotes	with
T9270	47140-47143	DT	denotes	the
T9272	47144-47152	VBN	denotes	inverted
T9273	47153-47158	NNP	denotes	Nikon
T9274	47159-47166	NN	denotes	TE2000U
T9271	47167-47177	NN	denotes	microscope
T9275	47178-47182	VBD	denotes	were
T9268	47183-47191	VBN	denotes	obtained
T9276	47192-47197	VBG	denotes	using
T9277	47198-47199	DT	denotes	a
T9279	47200-47203	NN	denotes	CCD
T9278	47204-47210	NN	denotes	camera
T9280	47211-47212	-LRB-	denotes	(
T9281	47212-47220	NNP	denotes	CoolSNAP
T9282	47221-47223	NNP	denotes	HQ
T9283	47223-47224	:	denotes	;
T9285	47225-47230	NNP	denotes	Roper
T9284	47231-47241	NNP	denotes	Scientific
T9286	47241-47243	,	denotes	,
T9287	47243-47250	NNP	denotes	Trenton
T9288	47250-47252	,	denotes	,
T9289	47252-47255	NNP	denotes	New
T9290	47256-47262	NNP	denotes	Jersey
T9291	47262-47264	,	denotes	,
T9292	47264-47270	NNP	denotes	United
T9293	47271-47277	NNP	denotes	States
T9294	47277-47278	-RRB-	denotes	)
T9295	47278-47279	.	denotes	.
T9296	47279-47435	sentence	denotes	Images acquired under identical acquisition parameters were analyzed using Metamorph Software v6.2 (Universal Imaging, Glendale, Wisconsin, United States).
T9297	47280-47286	NNS	denotes	Images
T9299	47287-47295	VBN	denotes	acquired
T9300	47296-47301	IN	denotes	under
T9301	47302-47311	JJ	denotes	identical
T9303	47312-47323	NN	denotes	acquisition
T9302	47324-47334	NNS	denotes	parameters
T9304	47335-47339	VBD	denotes	were
T9298	47340-47348	VBN	denotes	analyzed
T9305	47349-47354	VBG	denotes	using
T9306	47355-47364	NNP	denotes	Metamorph
T9308	47365-47373	NNP	denotes	Software
T9307	47374-47378	NN	denotes	v6.2
T9309	47379-47380	-LRB-	denotes	(
T9311	47380-47389	NNP	denotes	Universal
T9310	47390-47397	NNP	denotes	Imaging
T9312	47397-47399	,	denotes	,
T9313	47399-47407	NNP	denotes	Glendale
T9314	47407-47409	,	denotes	,
T9315	47409-47418	NNP	denotes	Wisconsin
T9316	47418-47420	,	denotes	,
T9317	47420-47426	NNP	denotes	United
T9318	47427-47433	NNP	denotes	States
T9319	47433-47434	-RRB-	denotes	)
T9320	47434-47435	.	denotes	.
T9321	47435-47624	sentence	denotes	Whenever applicable, serial optical Z-stacks (20–30 focal planes at 100-nm intervals) were captured and computationally processed by 3-D blind deconvolution methods with the same software.
T9322	47436-47444	RB	denotes	Whenever
T9323	47445-47455	JJ	denotes	applicable
T9325	47455-47457	,	denotes	,
T9326	47457-47463	JJ	denotes	serial
T9328	47464-47471	JJ	denotes	optical
T9329	47472-47473	NN	denotes	Z
T9330	47473-47474	HYPH	denotes	-
T9327	47474-47480	NNS	denotes	stacks
T9331	47481-47482	-LRB-	denotes	(
T9332	47482-47484	CD	denotes	20
T9334	47484-47485	SYM	denotes	–
T9333	47485-47487	CD	denotes	30
T9336	47488-47493	JJ	denotes	focal
T9335	47494-47500	NNS	denotes	planes
T9337	47501-47503	IN	denotes	at
T9338	47504-47507	CD	denotes	100
T9340	47507-47508	HYPH	denotes	-
T9339	47508-47510	NN	denotes	nm
T9341	47511-47520	NNS	denotes	intervals
T9342	47520-47521	-RRB-	denotes	)
T9343	47522-47526	VBD	denotes	were
T9324	47527-47535	VBN	denotes	captured
T9344	47536-47539	CC	denotes	and
T9345	47540-47555	RB	denotes	computationally
T9346	47556-47565	VBN	denotes	processed
T9347	47566-47568	IN	denotes	by
T9348	47569-47570	CD	denotes	3
T9350	47570-47571	HYPH	denotes	-
T9349	47571-47572	NN	denotes	D
T9352	47573-47578	JJ	denotes	blind
T9353	47579-47592	NN	denotes	deconvolution
T9351	47593-47600	NNS	denotes	methods
T9354	47601-47605	IN	denotes	with
T9355	47606-47609	DT	denotes	the
T9357	47610-47614	JJ	denotes	same
T9356	47615-47623	NN	denotes	software
T9358	47623-47624	.	denotes	.
T9491	47626-47636	NN	denotes	Generation
T9492	47637-47639	IN	denotes	of
T9493	47640-47641	DT	denotes	a
T9495	47642-47647	NN	denotes	mouse
T9494	47648-47652	NN	denotes	line
T9496	47653-47657	IN	denotes	with
T9497	47658-47659	DT	denotes	a
T9499	47660-47669	VBN	denotes	disrupted
T9500	47670-47676	NN	denotes	RanBP2
T9498	47677-47682	NN	denotes	locus
T9501	47683-47686	CC	denotes	and
T9502	47687-47699	JJ	denotes	histological
T9503	47700-47708	NN	denotes	analysis
T9504	47709-47711	IN	denotes	of
T9505	47712-47719	VBN	denotes	trapped
T9507	47720-47726	NN	denotes	RanBP2
T9506	47727-47731	NNS	denotes	mice
T9508	47731-47732	.	denotes	.
T9509	47732-48071	sentence	denotes	A feeder-independent mouse ES cell line derived from the 129P2/OlaHsd strain and harboring a disrupted RanBP2 locus by insertion mutagenesis with the gene trap vector, pGT0pfs [67,68] (generated by W. Skarnes), was obtained from the Mutant Mouse Regional Resource Center (University of California Davis, Davis, California, United States).
T9510	47733-47734	DT	denotes	A
T9512	47735-47741	NN	denotes	feeder
T9514	47741-47742	HYPH	denotes	-
T9513	47742-47753	JJ	denotes	independent
T9515	47754-47759	NN	denotes	mouse
T9516	47760-47762	NN	denotes	ES
T9517	47763-47767	NN	denotes	cell
T9511	47768-47772	NN	denotes	line
T9519	47773-47780	VBN	denotes	derived
T9520	47781-47785	IN	denotes	from
T9521	47786-47789	DT	denotes	the
T9523	47790-47795	NN	denotes	129P2
T9525	47795-47796	HYPH	denotes	/
T9524	47796-47802	NN	denotes	OlaHsd
T9522	47803-47809	NN	denotes	strain
T9526	47810-47813	CC	denotes	and
T9527	47814-47823	VBG	denotes	harboring
T9528	47824-47825	DT	denotes	a
T9530	47826-47835	VBN	denotes	disrupted
T9531	47836-47842	NN	denotes	RanBP2
T9529	47843-47848	NN	denotes	locus
T9532	47849-47851	IN	denotes	by
T9533	47852-47861	NN	denotes	insertion
T9534	47862-47873	NN	denotes	mutagenesis
T9535	47874-47878	IN	denotes	with
T9536	47879-47882	DT	denotes	the
T9538	47883-47887	NN	denotes	gene
T9539	47888-47892	NN	denotes	trap
T9537	47893-47899	NN	denotes	vector
T9540	47899-47901	,	denotes	,
T9541	47901-47908	NN	denotes	pGT0pfs
T9542	47909-47910	-LRB-	denotes	[
T9544	47910-47912	CD	denotes	67
T9545	47912-47913	,	denotes	,
T9543	47913-47915	CD	denotes	68
T9546	47915-47916	-RRB-	denotes	]
T9547	47917-47918	-LRB-	denotes	(
T9548	47918-47927	VBN	denotes	generated
T9549	47928-47930	IN	denotes	by
T9550	47931-47933	NNP	denotes	W.
T9551	47934-47941	NNP	denotes	Skarnes
T9552	47941-47942	-RRB-	denotes	)
T9553	47942-47944	,	denotes	,
T9554	47944-47947	VBD	denotes	was
T9518	47948-47956	VBN	denotes	obtained
T9555	47957-47961	IN	denotes	from
T9556	47962-47965	DT	denotes	the
T9558	47966-47972	NNP	denotes	Mutant
T9559	47973-47978	NNP	denotes	Mouse
T9560	47979-47987	NNP	denotes	Regional
T9561	47988-47996	NNP	denotes	Resource
T9557	47997-48003	NNP	denotes	Center
T9562	48004-48005	-LRB-	denotes	(
T9563	48005-48015	NNP	denotes	University
T9564	48016-48018	IN	denotes	of
T9565	48019-48029	NNP	denotes	California
T9566	48030-48035	NNP	denotes	Davis
T9567	48035-48037	,	denotes	,
T9568	48037-48042	NNP	denotes	Davis
T9569	48042-48044	,	denotes	,
T9570	48044-48054	NNP	denotes	California
T9571	48054-48056	,	denotes	,
T9572	48056-48062	NNP	denotes	United
T9573	48063-48069	NNP	denotes	States
T9574	48069-48070	-RRB-	denotes	)
T9575	48070-48071	.	denotes	.
T9576	48071-48301	sentence	denotes	The gene trap vector contains a promoterless neo-lacZ fusion gene with a splice acceptor site at the 5′ end followed by an internal ribosome entry site (IRES) and the human placental alkaline phosphatase (PLAP) reporter cassette.
T9577	48072-48075	DT	denotes	The
T9579	48076-48080	NN	denotes	gene
T9580	48081-48085	NN	denotes	trap
T9578	48086-48092	NN	denotes	vector
T9581	48093-48101	VBZ	denotes	contains
T9582	48102-48103	DT	denotes	a
T9584	48104-48116	JJ	denotes	promoterless
T9585	48117-48125	NN	denotes	neo-lacZ
T9586	48126-48132	NN	denotes	fusion
T9583	48133-48137	NN	denotes	gene
T9587	48138-48142	IN	denotes	with
T9588	48143-48144	DT	denotes	a
T9590	48145-48151	NN	denotes	splice
T9591	48152-48160	NN	denotes	acceptor
T9589	48161-48165	NN	denotes	site
T9592	48166-48168	IN	denotes	at
T9593	48169-48172	DT	denotes	the
T9595	48173-48174	CD	denotes	5
T9596	48174-48175	SYM	denotes	′
T9594	48176-48179	NN	denotes	end
T9597	48180-48188	VBN	denotes	followed
T9598	48189-48191	IN	denotes	by
T9599	48192-48194	DT	denotes	an
T9601	48195-48203	JJ	denotes	internal
T9602	48204-48212	NN	denotes	ribosome
T9603	48213-48218	NN	denotes	entry
T9600	48219-48223	NN	denotes	site
T9604	48224-48225	-LRB-	denotes	(
T9605	48225-48229	NN	denotes	IRES
T9606	48229-48230	-RRB-	denotes	)
T9607	48231-48234	CC	denotes	and
T9608	48235-48238	DT	denotes	the
T9610	48239-48244	JJ	denotes	human
T9611	48245-48254	JJ	denotes	placental
T9613	48255-48263	NN	denotes	alkaline
T9612	48264-48275	NN	denotes	phosphatase
T9614	48276-48277	-LRB-	denotes	(
T9615	48277-48281	NN	denotes	PLAP
T9616	48281-48282	-RRB-	denotes	)
T9617	48283-48291	NN	denotes	reporter
T9609	48292-48300	NN	denotes	cassette
T9618	48300-48301	.	denotes	.
T9619	48301-48405	sentence	denotes	The RanBP2 129Ola ES line was injected into C57BL/6J blastocysts and four male chimeras were generated.
T9620	48302-48305	DT	denotes	The
T9622	48306-48312	NN	denotes	RanBP2
T9623	48313-48319	NN	denotes	129Ola
T9624	48320-48322	NN	denotes	ES
T9621	48323-48327	NN	denotes	line
T9626	48328-48331	VBD	denotes	was
T9625	48332-48340	VBN	denotes	injected
T9627	48341-48345	IN	denotes	into
T9628	48346-48351	NN	denotes	C57BL
T9630	48351-48352	HYPH	denotes	/
T9629	48352-48354	NN	denotes	6J
T9631	48355-48366	NNS	denotes	blastocysts
T9632	48367-48370	CC	denotes	and
T9633	48371-48375	CD	denotes	four
T9635	48376-48380	JJ	denotes	male
T9634	48381-48389	NNS	denotes	chimeras
T9637	48390-48394	VBD	denotes	were
T9636	48395-48404	VBN	denotes	generated
T9638	48404-48405	.	denotes	.
T9639	48405-48563	sentence	denotes	These were bred into 129P2/OlaHsd (Harlan, Indianapolis, Indiana, United States) and C57BL/6J (Jackson Laboratory, Bar Harbor, Maine, United States) females.
T9640	48406-48411	DT	denotes	These
T9642	48412-48416	VBD	denotes	were
T9641	48417-48421	VBN	denotes	bred
T9643	48422-48426	IN	denotes	into
T9644	48427-48432	NN	denotes	129P2
T9646	48432-48433	HYPH	denotes	/
T9645	48433-48439	NN	denotes	OlaHsd
T9648	48440-48441	-LRB-	denotes	(
T9649	48441-48447	NNP	denotes	Harlan
T9650	48447-48449	,	denotes	,
T9651	48449-48461	NNP	denotes	Indianapolis
T9652	48461-48463	,	denotes	,
T9653	48463-48470	NNP	denotes	Indiana
T9654	48470-48472	,	denotes	,
T9655	48472-48478	NNP	denotes	United
T9656	48479-48485	NNP	denotes	States
T9657	48485-48486	-RRB-	denotes	)
T9658	48487-48490	CC	denotes	and
T9659	48491-48496	NN	denotes	C57BL
T9661	48496-48497	HYPH	denotes	/
T9660	48497-48499	NN	denotes	6J
T9662	48500-48501	-LRB-	denotes	(
T9664	48501-48508	NNP	denotes	Jackson
T9663	48509-48519	NNP	denotes	Laboratory
T9665	48519-48521	,	denotes	,
T9666	48521-48524	NNP	denotes	Bar
T9667	48525-48531	NNP	denotes	Harbor
T9668	48531-48533	,	denotes	,
T9669	48533-48538	NNP	denotes	Maine
T9670	48538-48540	,	denotes	,
T9671	48540-48546	NNP	denotes	United
T9672	48547-48553	NNP	denotes	States
T9673	48553-48554	-RRB-	denotes	)
T9647	48555-48562	NNS	denotes	females
T9674	48562-48563	.	denotes	.
T9675	48563-48779	sentence	denotes	The two resulting and independent F1s lines, with 129P2/OlaHsd inbred and 129P2/OlaHsd/C57BL/6J mixed genetic backgrounds, were tested for germline transmission by PCR and Southern blot analysis of tail genomic DNA.
T9676	48564-48567	DT	denotes	The
T9678	48568-48571	CD	denotes	two
T9679	48572-48581	VBG	denotes	resulting
T9680	48582-48585	CC	denotes	and
T9681	48586-48597	JJ	denotes	independent
T9682	48598-48601	NN	denotes	F1s
T9677	48602-48607	NNS	denotes	lines
T9684	48607-48609	,	denotes	,
T9685	48609-48613	IN	denotes	with
T9686	48614-48619	NN	denotes	129P2
T9688	48619-48620	HYPH	denotes	/
T9687	48620-48626	NN	denotes	OlaHsd
T9689	48627-48633	JJ	denotes	inbred
T9690	48634-48637	CC	denotes	and
T9691	48638-48643	NN	denotes	129P2
T9693	48643-48644	HYPH	denotes	/
T9694	48644-48650	NN	denotes	OlaHsd
T9695	48650-48651	HYPH	denotes	/
T9696	48651-48656	NN	denotes	C57BL
T9697	48656-48657	HYPH	denotes	/
T9692	48657-48659	NN	denotes	6J
T9699	48660-48665	JJ	denotes	mixed
T9700	48666-48673	JJ	denotes	genetic
T9698	48674-48685	NNS	denotes	backgrounds
T9701	48685-48687	,	denotes	,
T9702	48687-48691	VBD	denotes	were
T9683	48692-48698	VBN	denotes	tested
T9703	48699-48702	IN	denotes	for
T9704	48703-48711	NN	denotes	germline
T9705	48712-48724	NN	denotes	transmission
T9706	48725-48727	IN	denotes	by
T9707	48728-48731	NN	denotes	PCR
T9709	48732-48735	CC	denotes	and
T9710	48736-48744	NNP	denotes	Southern
T9711	48745-48749	NN	denotes	blot
T9708	48750-48758	NN	denotes	analysis
T9712	48759-48761	IN	denotes	of
T9713	48762-48766	NN	denotes	tail
T9715	48767-48774	JJ	denotes	genomic
T9714	48775-48778	NN	denotes	DNA
T9716	48778-48779	.	denotes	.
T9717	48779-49134	sentence	denotes	β-gal and PLAP activities in whole mount embryos and retinal sections were detected, respectively, by incubation with 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside and AP staining buffer (0.1 mg/ml 5-bromo-4-chloro-3-indolyl phosphate, 1 mg/ml nitroblue tetrazolium in 100 mM Tris-HCl (pH 9.5), 100 mM NaCl, 5 mM MgCl2) as described elsewhere [67,68].
T9718	48780-48781	NN	denotes	β
T9720	48781-48782	HYPH	denotes	-
T9719	48782-48785	NN	denotes	gal
T9722	48786-48789	CC	denotes	and
T9723	48790-48794	NN	denotes	PLAP
T9721	48795-48805	NNS	denotes	activities
T9725	48806-48808	IN	denotes	in
T9726	48809-48814	JJ	denotes	whole
T9727	48815-48820	NN	denotes	mount
T9728	48821-48828	NNS	denotes	embryos
T9729	48829-48832	CC	denotes	and
T9730	48833-48840	JJ	denotes	retinal
T9731	48841-48849	NNS	denotes	sections
T9732	48850-48854	VBD	denotes	were
T9724	48855-48863	VBN	denotes	detected
T9733	48863-48865	,	denotes	,
T9734	48865-48877	RB	denotes	respectively
T9735	48877-48879	,	denotes	,
T9736	48879-48881	IN	denotes	by
T9737	48882-48892	NN	denotes	incubation
T9738	48893-48897	IN	denotes	with
T9739	48898-48899	CD	denotes	5
T9741	48899-48900	HYPH	denotes	-
T9740	48900-48905	NN	denotes	bromo
T9743	48905-48906	HYPH	denotes	-
T9744	48906-48907	CD	denotes	4
T9745	48907-48908	HYPH	denotes	-
T9746	48908-48914	NN	denotes	chloro
T9747	48914-48915	HYPH	denotes	-
T9748	48915-48916	CD	denotes	3
T9750	48916-48917	HYPH	denotes	-
T9751	48917-48924	NN	denotes	indolyl
T9749	48925-48926	NN	denotes	β
T9752	48926-48927	HYPH	denotes	-
T9753	48927-48928	NN	denotes	D
T9754	48928-48929	HYPH	denotes	-
T9742	48929-48946	NN	denotes	galactopyranoside
T9756	48947-48950	CC	denotes	and
T9757	48951-48953	NN	denotes	AP
T9758	48954-48962	NN	denotes	staining
T9755	48963-48969	NN	denotes	buffer
T9759	48970-48971	-LRB-	denotes	(
T9761	48971-48974	CD	denotes	0.1
T9762	48975-48977	NNS	denotes	mg
T9764	48977-48978	SYM	denotes	/
T9765	48978-48980	NN	denotes	ml
T9766	48981-48982	CD	denotes	5
T9768	48982-48983	HYPH	denotes	-
T9767	48983-48988	NN	denotes	bromo
T9769	48988-48989	HYPH	denotes	-
T9770	48989-48990	CD	denotes	4
T9772	48990-48991	HYPH	denotes	-
T9771	48991-48997	NN	denotes	chloro
T9773	48997-48998	HYPH	denotes	-
T9774	48998-48999	CD	denotes	3
T9775	48999-49000	HYPH	denotes	-
T9776	49000-49007	NN	denotes	indolyl
T9763	49008-49017	NN	denotes	phosphate
T9777	49017-49019	,	denotes	,
T9778	49019-49020	CD	denotes	1
T9779	49021-49023	NNS	denotes	mg
T9780	49023-49024	SYM	denotes	/
T9781	49024-49026	NN	denotes	ml
T9782	49027-49036	JJ	denotes	nitroblue
T9760	49037-49048	NN	denotes	tetrazolium
T9783	49049-49051	IN	denotes	in
T9784	49052-49055	CD	denotes	100
T9785	49056-49058	NNS	denotes	mM
T9787	49059-49063	NN	denotes	Tris
T9788	49063-49064	HYPH	denotes	-
T9786	49064-49067	NN	denotes	HCl
T9789	49068-49069	-LRB-	denotes	(
T9790	49069-49071	NN	denotes	pH
T9791	49072-49075	CD	denotes	9.5
T9792	49075-49076	-RRB-	denotes	)
T9793	49076-49078	,	denotes	,
T9794	49078-49081	CD	denotes	100
T9795	49082-49084	NNS	denotes	mM
T9796	49085-49089	NN	denotes	NaCl
T9797	49089-49091	,	denotes	,
T9798	49091-49092	CD	denotes	5
T9799	49093-49095	NNS	denotes	mM
T9800	49096-49101	NN	denotes	MgCl2
T9801	49101-49102	-RRB-	denotes	)
T9802	49103-49105	IN	denotes	as
T9803	49106-49115	VBN	denotes	described
T9804	49116-49125	RB	denotes	elsewhere
T9805	49126-49127	-LRB-	denotes	[
T9807	49127-49129	CD	denotes	67
T9808	49129-49130	,	denotes	,
T9806	49130-49132	CD	denotes	68
T9809	49132-49133	-RRB-	denotes	]
T9810	49133-49134	.	denotes	.
T9862	49136-49143	NNP	denotes	Western
T9863	49144-49148	NN	denotes	blot
T9864	49149-49152	CC	denotes	and
T9865	49153-49165	NN	denotes	quantitation
T9866	49166-49174	NN	denotes	analysis
T9867	49175-49177	IN	denotes	of
T9868	49178-49185	NN	denotes	protein
T9869	49186-49196	NN	denotes	expression
T9870	49197-49200	CC	denotes	and
T9871	49201-49204	NN	denotes	ATP
T9872	49205-49211	NNS	denotes	levels
T9873	49211-49212	.	denotes	.
T9874	49212-49329	sentence	denotes	Homogenates and detergent solubilized extracts of tissues and immunoblots were prepared as described elsewhere [69].
T9875	49213-49224	NNS	denotes	Homogenates
T9877	49225-49228	CC	denotes	and
T9878	49229-49238	NN	denotes	detergent
T9879	49239-49250	VBN	denotes	solubilized
T9880	49251-49259	NNS	denotes	extracts
T9881	49260-49262	IN	denotes	of
T9882	49263-49270	NNS	denotes	tissues
T9883	49271-49274	CC	denotes	and
T9884	49275-49286	NNS	denotes	immunoblots
T9885	49287-49291	VBD	denotes	were
T9876	49292-49300	VBN	denotes	prepared
T9886	49301-49303	IN	denotes	as
T9887	49304-49313	VBN	denotes	described
T9888	49314-49323	RB	denotes	elsewhere
T9889	49324-49325	-LRB-	denotes	[
T9890	49325-49327	CD	denotes	69
T9891	49327-49328	-RRB-	denotes	]
T9892	49328-49329	.	denotes	.
T9893	49329-49527	sentence	denotes	Quantitation of immunoblot bands calibrated against internal marker bands, such as mHsp70 and Nup153, was carried out with Gel Pro Analyzer (MediaCybernetics, San Diego, California, United States).
T9894	49330-49342	NN	denotes	Quantitation
T9896	49343-49345	IN	denotes	of
T9897	49346-49356	NN	denotes	immunoblot
T9898	49357-49362	NNS	denotes	bands
T9899	49363-49373	VBN	denotes	calibrated
T9900	49374-49381	IN	denotes	against
T9901	49382-49390	JJ	denotes	internal
T9903	49391-49397	NN	denotes	marker
T9902	49398-49403	NNS	denotes	bands
T9904	49403-49405	,	denotes	,
T9905	49405-49409	JJ	denotes	such
T9906	49410-49412	IN	denotes	as
T9907	49413-49419	NN	denotes	mHsp70
T9908	49420-49423	CC	denotes	and
T9909	49424-49430	NN	denotes	Nup153
T9910	49430-49432	,	denotes	,
T9911	49432-49435	VBD	denotes	was
T9895	49436-49443	VBN	denotes	carried
T9912	49444-49447	RP	denotes	out
T9913	49448-49452	IN	denotes	with
T9914	49453-49456	NNP	denotes	Gel
T9916	49457-49460	NNP	denotes	Pro
T9915	49461-49469	NNP	denotes	Analyzer
T9917	49470-49471	-LRB-	denotes	(
T9918	49471-49487	NNPS	denotes	MediaCybernetics
T9919	49487-49489	,	denotes	,
T9920	49489-49492	NNP	denotes	San
T9921	49493-49498	NNP	denotes	Diego
T9922	49498-49500	,	denotes	,
T9923	49500-49510	NNP	denotes	California
T9924	49510-49512	,	denotes	,
T9925	49512-49518	NNP	denotes	United
T9926	49519-49525	NNP	denotes	States
T9927	49525-49526	-RRB-	denotes	)
T9928	49526-49527	.	denotes	.
T9929	49527-49743	sentence	denotes	For determination of ATP levels, freshly dissected and flash-frozen tissues were homogenized in 2.5% trichloroacetic acid (TCA), neutralized, and diluted to a final concentration of 0.1% with Tris-Acetate (pH 7.75).
T9930	49528-49531	IN	denotes	For
T9932	49532-49545	NN	denotes	determination
T9933	49546-49548	IN	denotes	of
T9934	49549-49552	NN	denotes	ATP
T9935	49553-49559	NNS	denotes	levels
T9936	49559-49561	,	denotes	,
T9937	49561-49568	RB	denotes	freshly
T9938	49569-49578	VBN	denotes	dissected
T9940	49579-49582	CC	denotes	and
T9941	49583-49588	NN	denotes	flash
T9943	49588-49589	HYPH	denotes	-
T9942	49589-49595	VBN	denotes	frozen
T9939	49596-49603	NNS	denotes	tissues
T9944	49604-49608	VBD	denotes	were
T9931	49609-49620	VBN	denotes	homogenized
T9945	49621-49623	IN	denotes	in
T9946	49624-49627	CD	denotes	2.5
T9947	49627-49628	NN	denotes	%
T9948	49629-49644	JJ	denotes	trichloroacetic
T9949	49645-49649	NN	denotes	acid
T9950	49650-49651	-LRB-	denotes	(
T9951	49651-49654	NN	denotes	TCA
T9952	49654-49655	-RRB-	denotes	)
T9953	49655-49657	,	denotes	,
T9954	49657-49668	VBN	denotes	neutralized
T9955	49668-49670	,	denotes	,
T9956	49670-49673	CC	denotes	and
T9957	49674-49681	VBD	denotes	diluted
T9958	49682-49684	IN	denotes	to
T9959	49685-49686	DT	denotes	a
T9961	49687-49692	JJ	denotes	final
T9960	49693-49706	NN	denotes	concentration
T9962	49707-49709	IN	denotes	of
T9963	49710-49713	CD	denotes	0.1
T9964	49713-49714	NN	denotes	%
T9965	49715-49719	IN	denotes	with
T9966	49720-49724	NN	denotes	Tris
T9968	49724-49725	HYPH	denotes	-
T9967	49725-49732	NN	denotes	Acetate
T9969	49733-49734	-LRB-	denotes	(
T9970	49734-49736	NN	denotes	pH
T9971	49737-49741	CD	denotes	7.75
T9972	49741-49742	-RRB-	denotes	)
T9973	49742-49743	.	denotes	.
T9974	49743-50008	sentence	denotes	ATP measurements were carried out with the ENLITEN ATP Assay System Bioluminescence Detection Kit for ATP (Promega, Madison, Wisconsin, United States) as per the manufacturer's instruction in SpectraMax-M5 (Molecular Devices, Sunnyvale, California, United States).
T9975	49744-49747	NN	denotes	ATP
T9976	49748-49760	NNS	denotes	measurements
T9978	49761-49765	VBD	denotes	were
T9977	49766-49773	VBN	denotes	carried
T9979	49774-49777	RP	denotes	out
T9980	49778-49782	IN	denotes	with
T9981	49783-49786	DT	denotes	the
T9983	49787-49794	NNP	denotes	ENLITEN
T9984	49795-49798	NNP	denotes	ATP
T9986	49799-49804	NNP	denotes	Assay
T9985	49805-49811	NNP	denotes	System
T9987	49812-49827	NNP	denotes	Bioluminescence
T9988	49828-49837	NNP	denotes	Detection
T9982	49838-49841	NNP	denotes	Kit
T9989	49842-49845	IN	denotes	for
T9990	49846-49849	NN	denotes	ATP
T9991	49850-49851	-LRB-	denotes	(
T9992	49851-49858	NNP	denotes	Promega
T9993	49858-49860	,	denotes	,
T9994	49860-49867	NNP	denotes	Madison
T9995	49867-49869	,	denotes	,
T9996	49869-49878	NNP	denotes	Wisconsin
T9997	49878-49880	,	denotes	,
T9998	49880-49886	NNP	denotes	United
T9999	49887-49893	NNP	denotes	States
T10000	49893-49894	-RRB-	denotes	)
T10001	49895-49897	IN	denotes	as
T10002	49898-49901	IN	denotes	per
T10003	49902-49905	DT	denotes	the
T10004	49906-49918	NN	denotes	manufacturer
T10006	49918-49920	POS	denotes	's
T10005	49921-49932	NN	denotes	instruction
T10007	49933-49935	IN	denotes	in
T10008	49936-49946	NNP	denotes	SpectraMax
T10010	49946-49947	HYPH	denotes	-
T10009	49947-49949	NNP	denotes	M5
T10011	49950-49951	-LRB-	denotes	(
T10013	49951-49960	NNP	denotes	Molecular
T10012	49961-49968	NNPS	denotes	Devices
T10014	49968-49970	,	denotes	,
T10015	49970-49979	NNP	denotes	Sunnyvale
T10016	49979-49981	,	denotes	,
T10017	49981-49991	NNP	denotes	California
T10018	49991-49993	,	denotes	,
T10019	49993-49999	NNP	denotes	United
T10020	50000-50006	NNP	denotes	States
T10021	50006-50007	-RRB-	denotes	)
T10022	50007-50008	.	denotes	.
T10023	50008-50132	sentence	denotes	Protein concentration was measured by the BCA method (BioRad, Hercules, California, United States) using BSA as a standard.
T10024	50009-50016	NN	denotes	Protein
T10025	50017-50030	NN	denotes	concentration
T10027	50031-50034	VBD	denotes	was
T10026	50035-50043	VBN	denotes	measured
T10028	50044-50046	IN	denotes	by
T10029	50047-50050	DT	denotes	the
T10031	50051-50054	NN	denotes	BCA
T10030	50055-50061	NN	denotes	method
T10032	50062-50063	-LRB-	denotes	(
T10033	50063-50069	NNP	denotes	BioRad
T10034	50069-50071	,	denotes	,
T10035	50071-50079	NNP	denotes	Hercules
T10036	50079-50081	,	denotes	,
T10037	50081-50091	NNP	denotes	California
T10038	50091-50093	,	denotes	,
T10039	50093-50099	NNP	denotes	United
T10040	50100-50106	NNP	denotes	States
T10041	50106-50107	-RRB-	denotes	)
T10042	50108-50113	VBG	denotes	using
T10043	50114-50117	NN	denotes	BSA
T10044	50118-50120	IN	denotes	as
T10045	50121-50122	DT	denotes	a
T10046	50123-50131	NN	denotes	standard
T10047	50131-50132	.	denotes	.
T10131	50134-50143	JJ	denotes	Metabolic
T10132	50144-50150	NNS	denotes	assays
T10133	50150-50151	.	denotes	.
T10134	50151-50231	sentence	denotes	Two groups of male mice were kept under separate diet since gestation or birth.
T10135	50152-50155	CD	denotes	Two
T10136	50156-50162	NNS	denotes	groups
T10138	50163-50165	IN	denotes	of
T10139	50166-50170	JJ	denotes	male
T10140	50171-50175	NNS	denotes	mice
T10141	50176-50180	VBD	denotes	were
T10137	50181-50185	VBN	denotes	kept
T10142	50186-50191	IN	denotes	under
T10143	50192-50200	JJ	denotes	separate
T10144	50201-50205	NN	denotes	diet
T10145	50206-50211	IN	denotes	since
T10146	50212-50221	NN	denotes	gestation
T10147	50222-50224	CC	denotes	or
T10148	50225-50230	NN	denotes	birth
T10149	50230-50231	.	denotes	.
T10150	50231-50447	sentence	denotes	One group was fed with a normal chow diet (PicoLab Rodent Diet 20 −5053; LabDiet, Richmond, Indiana, United States), while the other was placed on a higher energy and fat diet (PicoLab Mouse Diet 20 −5058; LabDiet).
T10151	50232-50235	CD	denotes	One
T10152	50236-50241	NN	denotes	group
T10154	50242-50245	VBD	denotes	was
T10153	50246-50249	VBN	denotes	fed
T10155	50250-50254	IN	denotes	with
T10156	50255-50256	DT	denotes	a
T10158	50257-50263	JJ	denotes	normal
T10159	50264-50268	NN	denotes	chow
T10157	50269-50273	NN	denotes	diet
T10160	50274-50275	-LRB-	denotes	(
T10161	50275-50282	NNP	denotes	PicoLab
T10163	50283-50289	NNP	denotes	Rodent
T10162	50290-50294	NNP	denotes	Diet
T10164	50295-50297	CD	denotes	20
T10165	50298-50299	HYPH	denotes	−
T10166	50299-50303	CD	denotes	5053
T10167	50303-50304	:	denotes	;
T10168	50305-50312	NNP	denotes	LabDiet
T10169	50312-50314	,	denotes	,
T10170	50314-50322	NNP	denotes	Richmond
T10171	50322-50324	,	denotes	,
T10172	50324-50331	NNP	denotes	Indiana
T10173	50331-50333	,	denotes	,
T10174	50333-50339	NNP	denotes	United
T10175	50340-50346	NNP	denotes	States
T10176	50346-50347	-RRB-	denotes	)
T10177	50347-50349	,	denotes	,
T10178	50349-50354	IN	denotes	while
T10180	50355-50358	DT	denotes	the
T10181	50359-50364	NN	denotes	other
T10182	50365-50368	VBD	denotes	was
T10179	50369-50375	VBN	denotes	placed
T10183	50376-50378	IN	denotes	on
T10184	50379-50380	DT	denotes	a
T10186	50381-50387	JJR	denotes	higher
T10187	50388-50394	NN	denotes	energy
T10188	50395-50398	CC	denotes	and
T10189	50399-50402	NN	denotes	fat
T10185	50403-50407	NN	denotes	diet
T10190	50408-50409	-LRB-	denotes	(
T10191	50409-50416	NNP	denotes	PicoLab
T10193	50417-50422	NNP	denotes	Mouse
T10192	50423-50427	NNP	denotes	Diet
T10194	50428-50430	CD	denotes	20
T10195	50431-50432	HYPH	denotes	−
T10196	50432-50436	CD	denotes	5058
T10197	50436-50437	:	denotes	;
T10198	50438-50445	NNP	denotes	LabDiet
T10199	50445-50446	-RRB-	denotes	)
T10200	50446-50447	.	denotes	.
T10201	50447-50579	sentence	denotes	Glucose tolerance test was performed on overnight-fasted mice injected intraperitoneally with 2 g of glucose per kg of body weight.
T10202	50448-50455	NN	denotes	Glucose
T10203	50456-50465	NN	denotes	tolerance
T10204	50466-50470	NN	denotes	test
T10206	50471-50474	VBD	denotes	was
T10205	50475-50484	VBN	denotes	performed
T10207	50485-50487	IN	denotes	on
T10208	50488-50497	JJ	denotes	overnight
T10210	50497-50498	HYPH	denotes	-
T10209	50498-50504	VBN	denotes	fasted
T10211	50505-50509	NNS	denotes	mice
T10212	50510-50518	VBN	denotes	injected
T10213	50519-50536	RB	denotes	intraperitoneally
T10214	50537-50541	IN	denotes	with
T10215	50542-50543	CD	denotes	2
T10216	50544-50545	NNS	denotes	g
T10217	50546-50548	IN	denotes	of
T10218	50549-50556	NN	denotes	glucose
T10219	50557-50560	IN	denotes	per
T10220	50561-50563	NN	denotes	kg
T10221	50564-50566	IN	denotes	of
T10222	50567-50571	NN	denotes	body
T10223	50572-50578	NN	denotes	weight
T10224	50578-50579	.	denotes	.
T10225	50579-50776	sentence	denotes	Venous blood glucose values were determined at immediately before (0 min), and 15, 30, 60, and 120 min after the injection with an automatic glucose monitor instrument (One Touch Ultra, Lifescan).
T10226	50580-50586	JJ	denotes	Venous
T10228	50587-50592	NN	denotes	blood
T10229	50593-50600	NN	denotes	glucose
T10227	50601-50607	NNS	denotes	values
T10231	50608-50612	VBD	denotes	were
T10230	50613-50623	VBN	denotes	determined
T10232	50624-50626	IN	denotes	at
T10233	50627-50638	RB	denotes	immediately
T10234	50639-50645	IN	denotes	before
T10235	50646-50647	-LRB-	denotes	(
T10237	50647-50648	CD	denotes	0
T10236	50649-50652	NNS	denotes	min
T10238	50652-50653	-RRB-	denotes	)
T10239	50653-50655	,	denotes	,
T10240	50655-50658	CC	denotes	and
T10241	50659-50661	CD	denotes	15
T10243	50661-50663	,	denotes	,
T10244	50663-50665	CD	denotes	30
T10245	50665-50667	,	denotes	,
T10246	50667-50669	CD	denotes	60
T10247	50669-50671	,	denotes	,
T10248	50671-50674	CC	denotes	and
T10249	50675-50678	CD	denotes	120
T10242	50679-50682	NN	denotes	min
T10251	50683-50688	IN	denotes	after
T10252	50689-50692	DT	denotes	the
T10250	50693-50702	NN	denotes	injection
T10253	50703-50707	IN	denotes	with
T10254	50708-50710	DT	denotes	an
T10256	50711-50720	JJ	denotes	automatic
T10257	50721-50728	NN	denotes	glucose
T10258	50729-50736	NN	denotes	monitor
T10255	50737-50747	NN	denotes	instrument
T10259	50748-50749	-LRB-	denotes	(
T10260	50749-50752	CD	denotes	One
T10262	50753-50758	NNP	denotes	Touch
T10261	50759-50764	NNP	denotes	Ultra
T10263	50764-50766	,	denotes	,
T10264	50766-50774	NNP	denotes	Lifescan
T10265	50774-50775	-RRB-	denotes	)
T10266	50775-50776	.	denotes	.
T10267	50776-50989	sentence	denotes	Insulin tolerance test was performed the same way as that described for the glucose tolerance test with the exception that human insulin (0.75 U/kg of body mass) was injected intraperitoneally in non-fasted mice.
T10268	50777-50784	NN	denotes	Insulin
T10269	50785-50794	NN	denotes	tolerance
T10270	50795-50799	NN	denotes	test
T10272	50800-50803	VBD	denotes	was
T10271	50804-50813	VBN	denotes	performed
T10273	50814-50817	DT	denotes	the
T10275	50818-50822	JJ	denotes	same
T10274	50823-50826	NN	denotes	way
T10276	50827-50829	IN	denotes	as
T10277	50830-50834	DT	denotes	that
T10278	50835-50844	VBN	denotes	described
T10279	50845-50848	IN	denotes	for
T10280	50849-50852	DT	denotes	the
T10282	50853-50860	NN	denotes	glucose
T10283	50861-50870	NN	denotes	tolerance
T10281	50871-50875	NN	denotes	test
T10284	50876-50880	IN	denotes	with
T10285	50881-50884	DT	denotes	the
T10286	50885-50894	NN	denotes	exception
T10287	50895-50899	IN	denotes	that
T10289	50900-50905	JJ	denotes	human
T10290	50906-50913	NN	denotes	insulin
T10291	50914-50915	-LRB-	denotes	(
T10293	50915-50919	CD	denotes	0.75
T10292	50920-50921	NNS	denotes	U
T10294	50921-50922	SYM	denotes	/
T10295	50922-50924	NN	denotes	kg
T10296	50925-50927	IN	denotes	of
T10297	50928-50932	NN	denotes	body
T10298	50933-50937	NN	denotes	mass
T10299	50937-50938	-RRB-	denotes	)
T10300	50939-50942	VBD	denotes	was
T10288	50943-50951	VBN	denotes	injected
T10301	50952-50969	RB	denotes	intraperitoneally
T10302	50970-50972	IN	denotes	in
T10303	50973-50983	JJ	denotes	non-fasted
T10304	50984-50988	NNS	denotes	mice
T10305	50988-50989	.	denotes	.
T10306	50989-51221	sentence	denotes	Pyruvate challenge test was performed the same way as that described for the glucose tolerance test with the exception that pyruvate dissolved in saline (2 g/kg of body mass) was injected intraperitoneally in overnight-fasted mice.
T10307	50990-50998	NN	denotes	Pyruvate
T10308	50999-51008	NN	denotes	challenge
T10309	51009-51013	NN	denotes	test
T10311	51014-51017	VBD	denotes	was
T10310	51018-51027	VBN	denotes	performed
T10312	51028-51031	DT	denotes	the
T10314	51032-51036	JJ	denotes	same
T10313	51037-51040	NN	denotes	way
T10315	51041-51043	IN	denotes	as
T10316	51044-51048	DT	denotes	that
T10317	51049-51058	VBN	denotes	described
T10318	51059-51062	IN	denotes	for
T10319	51063-51066	DT	denotes	the
T10321	51067-51074	NN	denotes	glucose
T10322	51075-51084	NN	denotes	tolerance
T10320	51085-51089	NN	denotes	test
T10323	51090-51094	IN	denotes	with
T10324	51095-51098	DT	denotes	the
T10325	51099-51108	NN	denotes	exception
T10326	51109-51113	IN	denotes	that
T10328	51114-51122	NN	denotes	pyruvate
T10329	51123-51132	VBN	denotes	dissolved
T10330	51133-51135	IN	denotes	in
T10331	51136-51142	NN	denotes	saline
T10332	51143-51144	-LRB-	denotes	(
T10334	51144-51145	CD	denotes	2
T10333	51146-51147	NNS	denotes	g
T10335	51147-51148	SYM	denotes	/
T10336	51148-51150	NN	denotes	kg
T10337	51151-51153	IN	denotes	of
T10338	51154-51158	NN	denotes	body
T10339	51159-51163	NN	denotes	mass
T10340	51163-51164	-RRB-	denotes	)
T10341	51165-51168	VBD	denotes	was
T10327	51169-51177	VBN	denotes	injected
T10342	51178-51195	RB	denotes	intraperitoneally
T10343	51196-51198	IN	denotes	in
T10344	51199-51208	JJ	denotes	overnight
T10346	51208-51209	HYPH	denotes	-
T10345	51209-51215	VBN	denotes	fasted
T10347	51216-51220	NNS	denotes	mice
T10348	51220-51221	.	denotes	.
T10407	51223-51242	NN	denotes	Electroretinography
T10408	51242-51243	.	denotes	.
T10409	51243-51414	sentence	denotes	Five ~6-mo-old male RanBP2+/+ and RanBP2+/− mice in 129ola background were kept in dim cyclic light (10 lux) for 2 wk before electroretinographic responses were recorded.
T10410	51244-51248	CD	denotes	Five
T10412	51249-51250	SYM	denotes	~
T10413	51250-51251	CD	denotes	6
T10415	51251-51252	HYPH	denotes	-
T10414	51252-51254	NN	denotes	mo
T10417	51254-51255	HYPH	denotes	-
T10416	51255-51258	JJ	denotes	old
T10418	51259-51263	JJ	denotes	male
T10419	51264-51270	NN	denotes	RanBP2
T10420	51270-51271	SYM	denotes	+
T10421	51271-51272	HYPH	denotes	/
T10422	51272-51273	SYM	denotes	+
T10423	51274-51277	CC	denotes	and
T10424	51278-51284	NN	denotes	RanBP2
T10425	51284-51285	SYM	denotes	+
T10426	51285-51286	HYPH	denotes	/
T10427	51286-51287	SYM	denotes	−
T10411	51288-51292	NNS	denotes	mice
T10429	51293-51295	IN	denotes	in
T10430	51296-51302	NN	denotes	129ola
T10431	51303-51313	NN	denotes	background
T10432	51314-51318	VBD	denotes	were
T10428	51319-51323	VBN	denotes	kept
T10433	51324-51326	IN	denotes	in
T10434	51327-51330	NN	denotes	dim
T10436	51331-51337	JJ	denotes	cyclic
T10435	51338-51343	NN	denotes	light
T10437	51344-51345	-LRB-	denotes	(
T10439	51345-51347	CD	denotes	10
T10438	51348-51351	NN	denotes	lux
T10440	51351-51352	-RRB-	denotes	)
T10441	51353-51356	IN	denotes	for
T10442	51357-51358	CD	denotes	2
T10443	51359-51361	NNS	denotes	wk
T10444	51362-51368	IN	denotes	before
T10446	51369-51389	JJ	denotes	electroretinographic
T10447	51390-51399	NNS	denotes	responses
T10448	51400-51404	VBD	denotes	were
T10445	51405-51413	VBN	denotes	recorded
T10449	51413-51414	.	denotes	.
T10450	51414-51508	sentence	denotes	The mice were anesthetized intraperitoneally with ketamine (80 mg/kg) and xylazine (4 mg/kg).
T10451	51415-51418	DT	denotes	The
T10452	51419-51423	NNS	denotes	mice
T10454	51424-51428	VBD	denotes	were
T10453	51429-51441	VBN	denotes	anesthetized
T10455	51442-51459	RB	denotes	intraperitoneally
T10456	51460-51464	IN	denotes	with
T10457	51465-51473	NN	denotes	ketamine
T10458	51474-51475	-LRB-	denotes	(
T10460	51475-51477	CD	denotes	80
T10459	51478-51480	NNS	denotes	mg
T10461	51480-51481	SYM	denotes	/
T10462	51481-51483	NN	denotes	kg
T10463	51483-51484	-RRB-	denotes	)
T10464	51485-51488	CC	denotes	and
T10465	51489-51497	NN	denotes	xylazine
T10466	51498-51499	-LRB-	denotes	(
T10468	51499-51500	CD	denotes	4
T10467	51501-51503	NNS	denotes	mg
T10469	51503-51504	SYM	denotes	/
T10470	51504-51506	NN	denotes	kg
T10471	51506-51507	-RRB-	denotes	)
T10472	51507-51508	.	denotes	.
T10473	51508-51600	sentence	denotes	Body temperature was maintained with a heating pad at 37.5 ± 1 °C throughout the recording.
T10474	51509-51513	NN	denotes	Body
T10475	51514-51525	NN	denotes	temperature
T10477	51526-51529	VBD	denotes	was
T10476	51530-51540	VBN	denotes	maintained
T10478	51541-51545	IN	denotes	with
T10479	51546-51547	DT	denotes	a
T10481	51548-51555	NN	denotes	heating
T10480	51556-51559	NN	denotes	pad
T10482	51560-51562	IN	denotes	at
T10483	51563-51567	CD	denotes	37.5
T10485	51568-51569	SYM	denotes	±
T10484	51570-51571	CD	denotes	1
T10486	51572-51574	NN	denotes	°C
T10487	51575-51585	IN	denotes	throughout
T10488	51586-51589	DT	denotes	the
T10489	51590-51599	NN	denotes	recording
T10490	51599-51600	.	denotes	.
T10491	51600-51724	sentence	denotes	The pupils were fully dilated with 0.5% tropicamide and 2.5% phenylephrine HCl, and both eyes were recorded simultaneously.
T10492	51601-51604	DT	denotes	The
T10493	51605-51611	NNS	denotes	pupils
T10495	51612-51616	VBD	denotes	were
T10496	51617-51622	RB	denotes	fully
T10494	51623-51630	VBN	denotes	dilated
T10497	51631-51635	IN	denotes	with
T10498	51636-51639	CD	denotes	0.5
T10499	51639-51640	NN	denotes	%
T10500	51641-51652	NN	denotes	tropicamide
T10501	51653-51656	CC	denotes	and
T10502	51657-51660	CD	denotes	2.5
T10503	51660-51661	NN	denotes	%
T10505	51662-51675	NN	denotes	phenylephrine
T10504	51676-51679	NN	denotes	HCl
T10506	51679-51681	,	denotes	,
T10507	51681-51684	CC	denotes	and
T10508	51685-51689	DT	denotes	both
T10509	51690-51694	NNS	denotes	eyes
T10511	51695-51699	VBD	denotes	were
T10510	51700-51708	VBN	denotes	recorded
T10512	51709-51723	RB	denotes	simultaneously
T10513	51723-51724	.	denotes	.
T10514	51724-51841	sentence	denotes	Corneal gold-wire loops were used as the active electrodes under 0.5% proparacaine hydrochloride topical anesthesia.
T10515	51725-51732	JJ	denotes	Corneal
T10517	51733-51737	JJ	denotes	gold
T10519	51737-51738	HYPH	denotes	-
T10518	51738-51742	NN	denotes	wire
T10516	51743-51748	NNS	denotes	loops
T10521	51749-51753	VBD	denotes	were
T10520	51754-51758	VBN	denotes	used
T10522	51759-51761	IN	denotes	as
T10523	51762-51765	DT	denotes	the
T10525	51766-51772	JJ	denotes	active
T10524	51773-51783	NNS	denotes	electrodes
T10526	51784-51789	IN	denotes	under
T10527	51790-51793	CD	denotes	0.5
T10528	51793-51794	NN	denotes	%
T10530	51795-51807	NN	denotes	proparacaine
T10529	51808-51821	NN	denotes	hydrochloride
T10532	51822-51829	JJ	denotes	topical
T10531	51830-51840	NN	denotes	anesthesia
T10533	51840-51841	.	denotes	.
T10534	51841-51931	sentence	denotes	Gold-wire electrodes placed on the sclera near the limbus served as reference electrodes.
T10535	51842-51846	JJ	denotes	Gold
T10537	51846-51847	HYPH	denotes	-
T10536	51847-51851	NN	denotes	wire
T10538	51852-51862	NNS	denotes	electrodes
T10540	51863-51869	VBN	denotes	placed
T10541	51870-51872	IN	denotes	on
T10542	51873-51876	DT	denotes	the
T10543	51877-51883	NN	denotes	sclera
T10544	51884-51888	IN	denotes	near
T10545	51889-51892	DT	denotes	the
T10546	51893-51899	NN	denotes	limbus
T10539	51900-51906	VBD	denotes	served
T10547	51907-51909	IN	denotes	as
T10548	51910-51919	NN	denotes	reference
T10549	51920-51930	NNS	denotes	electrodes
T10550	51930-51931	.	denotes	.
T10551	51931-51970	sentence	denotes	A ground wire was attached to the ear.
T10552	51932-51933	DT	denotes	A
T10554	51934-51940	NN	denotes	ground
T10553	51941-51945	NN	denotes	wire
T10556	51946-51949	VBD	denotes	was
T10555	51950-51958	VBN	denotes	attached
T10557	51959-51961	IN	denotes	to
T10558	51962-51965	DT	denotes	the
T10559	51966-51969	NN	denotes	ear
T10560	51969-51970	.	denotes	.
T10561	51970-52115	sentence	denotes	Scotopic responses were recorded from threshold to 9 log units of intensity above threshold, amplified, and filtered (5,000 gain, 0.1–1,000 Hz).
T10562	51971-51979	JJ	denotes	Scotopic
T10563	51980-51989	NNS	denotes	responses
T10565	51990-51994	VBD	denotes	were
T10564	51995-52003	VBN	denotes	recorded
T10566	52004-52008	IN	denotes	from
T10567	52009-52018	NN	denotes	threshold
T10568	52019-52021	IN	denotes	to
T10569	52022-52023	CD	denotes	9
T10571	52024-52027	NN	denotes	log
T10570	52028-52033	NNS	denotes	units
T10572	52034-52036	IN	denotes	of
T10573	52037-52046	NN	denotes	intensity
T10574	52047-52052	IN	denotes	above
T10575	52053-52062	NN	denotes	threshold
T10576	52062-52064	,	denotes	,
T10577	52064-52073	VBN	denotes	amplified
T10578	52073-52075	,	denotes	,
T10579	52075-52078	CC	denotes	and
T10580	52079-52087	VBN	denotes	filtered
T10581	52088-52089	-LRB-	denotes	(
T10583	52089-52094	CD	denotes	5,000
T10584	52095-52099	NN	denotes	gain
T10585	52099-52101	,	denotes	,
T10586	52101-52104	CD	denotes	0.1
T10588	52104-52105	SYM	denotes	–
T10587	52105-52110	CD	denotes	1,000
T10582	52111-52113	NN	denotes	Hz
T10589	52113-52114	-RRB-	denotes	)
T10590	52114-52115	.	denotes	.
T10591	52115-52203	sentence	denotes	For photopic recordings background light at 34 cd/m2 was used to suppress rod function.
T10592	52116-52119	IN	denotes	For
T10594	52120-52128	JJ	denotes	photopic
T10595	52129-52139	NNS	denotes	recordings
T10596	52140-52150	NN	denotes	background
T10597	52151-52156	NN	denotes	light
T10598	52157-52159	IN	denotes	at
T10599	52160-52162	CD	denotes	34
T10600	52163-52165	NNS	denotes	cd
T10601	52165-52166	SYM	denotes	/
T10602	52166-52168	NN	denotes	m2
T10603	52169-52172	VBD	denotes	was
T10593	52173-52177	VBN	denotes	used
T10604	52178-52180	TO	denotes	to
T10605	52181-52189	VB	denotes	suppress
T10606	52190-52193	NN	denotes	rod
T10607	52194-52202	NN	denotes	function
T10608	52202-52203	.	denotes	.
R1	T305	T304	pobj	RanBP2,of
R10	T239	T232	cc	and,Modulates
R100	T340	T341	nsubj	RanBP2,associates
R1000	T1730	T1715	parataxis	25,leads
R1001	T1731	T1730	punct	],25
R1002	T1732	T1715	punct	.,leads
R1003	T1734	T1735	mark	While,are
R1004	T1735	T1746	advcl	are,exhibit
R1005	T1736	T1737	det	the,effects
R1006	T1737	T1735	nsubj	effects,are
R1007	T1738	T1737	amod	dopaminergic,effects
R1008	T1739	T1740	amod	neuronal,restricted
R1009	T1740	T1737	amod	restricted,effects
R101	T342	T343	advmod	in,vitro
R1010	T1741	T1740	punct	-,restricted
R1011	T1742	T1737	prep	of,effects
R1012	T1743	T1742	pobj	mutations,of
R1013	T1744	T1743	prep	in,mutations
R1014	T1745	T1744	pobj	parkin,in
R1015	T1747	T1735	neg	not,are
R1016	T1748	T1735	acomp	understood,are
R1017	T1749	T1750	punct	[,25
R1018	T1750	T1735	parataxis	25,are
R1019	T1751	T1750	punct	],25
R102	T343	T341	advmod	vitro,associates
R1020	T1752	T1746	punct	", ",exhibit
R1021	T1753	T1754	nmod	parkin,mice
R1022	T1754	T1746	nsubj	mice,exhibit
R1023	T1755	T1753	punct	−,parkin
R1024	T1756	T1753	punct	/,parkin
R1025	T1757	T1753	punct	−,parkin
R1026	T1758	T1759	amod	mitochondrial,dysfunction
R1027	T1759	T1746	dobj	dysfunction,exhibit
R1028	T1760	T1759	cc	and,dysfunction
R1029	T1761	T1762	nmod	energy,deficits
R103	T344	T343	cc	and,vitro
R1030	T1762	T1759	conj	deficits,dysfunction
R1031	T1763	T1761	cc	and,energy
R1032	T1764	T1761	conj	growth,energy
R1033	T1765	T1746	punct	", ",exhibit
R1034	T1766	T1746	cc	and,exhibit
R1035	T1767	T1768	det	a,fraction
R1036	T1768	T1769	nsubj	fraction,localizes
R1037	T1815	T1814	pobj	RanBP2,in
R1038	T1769	T1746	conj	localizes,exhibit
R1039	T1816	T1815	cc	and,RanBP2
R104	T345	T346	advmod	in,vivo
R1040	T1770	T1768	prep	of,fraction
R1041	T1817	T1815	conj	parkin,RanBP2
R1042	T1818	T1812	aux	may,share
R1043	T1819	T1820	punct	(,physiological
R1044	T1771	T1770	pobj	parkin,of
R1045	T1820	T1823	amod	physiological,pathways
R1046	T1821	T1820	advmod	patho,physiological
R1047	T1772	T1769	prep	to,localizes
R1048	T1822	T1820	punct	),physiological
R1049	T1823	T1812	dobj	pathways,share
R105	T346	T343	conj	vivo,vitro
R1050	T1824	T1812	cc	and,share
R1051	T1825	T1812	conj	support,share
R1052	T1826	T1827	det	a,role
R1053	T1827	T1825	dobj	role,support
R1054	T1773	T1774	det	the,face
R1055	T1828	T1827	amod	multifaceted,role
R1056	T1829	T1827	prep	of,role
R1057	T1830	T1829	pobj	RanBP2,of
R1058	T1831	T1810	punct	.,suggest
R1059	T1774	T1772	pobj	face,to
R106	T347	T341	cc	and,associates
R1060	T1833	T1834	advmod	Yet,remain
R1061	T1775	T1774	amod	cytoplasmic,face
R1062	T1835	T1836	amod	clear,functions
R1063	T1836	T1834	nsubj	functions,remain
R1064	T1776	T1774	prep	of,face
R1065	T1837	T1836	prep	of,functions
R1066	T1838	T1837	pobj	RanBP2,of
R1067	T1839	T1836	prep	in,functions
R1068	T1777	T1778	det	the,mitochondria
R1069	T1840	T1841	nmod	animal,physiology
R107	T348	T341	conj	colocalizes,associates
R1070	T1841	T1839	pobj	physiology,in
R1071	T1778	T1776	pobj	mitochondria,of
R1072	T1842	T1840	cc	and,animal
R1073	T1843	T1840	conj	cell,animal
R1074	T1844	T1834	acomp	elusive,remain
R1075	T1779	T1774	punct	", ",face
R1076	T1845	T1834	punct	.,remain
R1077	T1847	T1848	advmod	Here,report
R1078	T1780	T1781	advmod	where,thought
R1079	T1781	T1774	relcl	thought,face
R108	T349	T348	prep	with,colocalizes
R1080	T1849	T1848	punct	", ",report
R1081	T1850	T1848	nsubj	we,report
R1082	T1851	T1848	prep	on,report
R1083	T1782	T1781	advmod	together,thought
R1084	T1852	T1853	det	the,identification
R1085	T1783	T1782	prep	with,together
R1086	T1853	T1851	pobj	identification,on
R1087	T1854	T1853	cc	and,identification
R1088	T1855	T1853	conj	function,identification
R1089	T1784	T1785	amod	other,partners
R109	T350	T351	det	the,metallochaperone
R1090	T1856	T1853	prep	of,identification
R1091	T1857	T1858	nummod	two,partners
R1092	T1785	T1783	pobj	partners,with
R1093	T1858	T1856	pobj	partners,of
R1094	T1859	T1858	amod	novel,partners
R1095	T1860	T1858	prep	of,partners
R1096	T1786	T1781	auxpass	is,thought
R1097	T1861	T1860	pobj	RanBP2,of
R1098	T1862	T1858	punct	", ",partners
R1099	T1787	T1788	aux	to,promote
R11	T240	T241	nsubj	Haploinsufficiency,Causes
R110	T351	T349	pobj	metallochaperone,with
R1100	T1863	T1858	appos	Cox11,partners
R1101	T1864	T1863	cc	and,Cox11
R1102	T1865	T1866	compound	hexokinase,type
R1103	T1788	T1781	xcomp	promote,thought
R1104	T1866	T1863	conj	type,Cox11
R1105	T1867	T1866	nummod	I,type
R1106	T1868	T1866	punct	(,type
R1107	T1869	T1866	appos	HKI,type
R1108	T1870	T1858	punct	),partners
R1109	T1871	T1858	punct	", ",partners
R111	T352	T351	amod	mitochondrial,metallochaperone
R1110	T1789	T1790	det	the,degradation
R1111	T1872	T1873	dep	which,interact
R1112	T1873	T1858	relcl	interact,partners
R1113	T1874	T1873	prep	with,interact
R1114	T1790	T1788	dobj	degradation,promote
R1115	T1875	T1876	det	a,domain
R1116	T1876	T1874	pobj	domain,with
R1117	T1877	T1876	amod	large,domain
R1118	T1878	T1877	cc	and,large
R1119	T1791	T1790	prep	of,degradation
R112	T353	T351	punct	", ",metallochaperone
R1120	T1879	T1877	conj	orphan,large
R1121	T1880	T1876	prep	of,domain
R1122	T1792	T1793	amod	selective,substrates
R1123	T1881	T1880	pobj	RanBP2,of
R1124	T1882	T1876	punct	", ",domain
R1125	T1883	T1884	det	the,domain
R1126	T1793	T1791	pobj	substrates,of
R1127	T1884	T1876	appos	domain,domain
R1128	T1885	T1886	npadvmod	leucine,rich
R1129	T1886	T1884	amod	rich,domain
R113	T354	T351	appos	Cox11,metallochaperone
R1130	T1794	T1793	amod	mitochondrial,substrates
R1131	T1887	T1886	punct	-,rich
R1132	T1795	T1788	cc	and,promote
R1133	T1888	T1884	punct	(,domain
R1134	T1889	T1884	appos	LD,domain
R1135	T1890	T1851	punct	),on
R1136	T1891	T1851	punct	;,on
R1137	T1796	T1797	aux	to,exert
R1138	T1892	T1851	cc	and,on
R1139	T1893	T1851	conj	on,on
R114	T355	T351	punct	", ",metallochaperone
R1140	T1894	T1895	det	the,outcome
R1141	T1797	T1788	conj	exert,promote
R1142	T1895	T1893	pobj	outcome,on
R1143	T1896	T1895	prep	of,outcome
R1144	T1798	T1799	det	a,function
R1145	T1799	T1797	dobj	function,exert
R1146	T1897	T1898	amod	partial,loss
R1147	T1898	T1896	pobj	loss,of
R1148	T1800	T1799	amod	neuroprotective,function
R1149	T1899	T1898	punct	-,loss
R115	T356	T351	cc	and,metallochaperone
R1150	T1900	T1898	prep	of,loss
R1151	T1901	T1900	punct	-,of
R1152	T1801	T1802	punct	[,26
R1153	T1902	T1900	pobj	function,of
R1154	T1903	T1898	prep	of,loss
R1155	T1904	T1903	pobj	RanBP2,of
R1156	T1905	T1895	prep	on,outcome
R1157	T1906	T1905	pobj	HKI,on
R1158	T1907	T1906	punct	", ",HKI
R1159	T1802	T1797	parataxis	26,exert
R116	T357	T358	det	the,pacemaker
R1160	T1908	T1909	compound	animal,physiology
R1161	T1909	T1906	conj	physiology,HKI
R1162	T1910	T1909	punct	", ",physiology
R1163	T1803	T1804	punct	–,29
R1164	T1911	T1909	cc	and,physiology
R1165	T1912	T1913	compound	glucose,energy
R1166	T1913	T1915	compound	energy,metabolism
R1167	T1804	T1802	prep	29,26
R1168	T1914	T1913	punct	/,energy
R1169	T1915	T1909	conj	metabolism,physiology
R117	T358	T351	conj	pacemaker,metallochaperone
R1170	T1805	T1802	punct	],26
R1171	T1916	T1848	punct	.,report
R1172	T1806	T1769	punct	.,localizes
R1173	T1808	T1809	det	These,data
R1174	T1809	T1810	nsubj	data,suggest
R1175	T1811	T1812	mark	that,share
R1176	T1812	T1810	ccomp	share,suggest
R1177	T1813	T1812	nsubj	deficits,share
R1178	T1814	T1813	prep	in,deficits
R1179	T2102	T2103	det	The,LD
R118	T359	T358	prep	of,pacemaker
R1180	T2103	T2104	nsubj	LD,Interacts
R1181	T2105	T2103	prep	of,LD
R1182	T2106	T2105	pobj	RanBP2,of
R1183	T2107	T2104	prep	with,Interacts
R1184	T2108	T2107	pobj	Cox11,with
R1185	T2109	T2108	cc	and,Cox11
R1186	T2110	T2108	conj	HKI,Cox11
R1187	T2112	T2113	det	The,LD
R1188	T2113	T2114	nsubj	LD,is
R1189	T2115	T2113	prep	of,LD
R119	T360	T359	pobj	glycolysis,of
R1190	T2116	T2115	pobj	RanBP2,of
R1191	T2117	T2118	punct	(,1A
R1192	T2118	T2113	parataxis	1A,LD
R1193	T2119	T2118	compound	Figure,1A
R1194	T2120	T2118	punct	),1A
R1195	T2121	T2122	det	a,domain
R1196	T2122	T2114	attr	domain,is
R1197	T2123	T2122	amod	large,domain
R1198	T2124	T2123	cc	and,large
R1199	T2125	T2123	conj	orphan,large
R12	T241	T232	conj	Causes,Modulates
R120	T361	T358	punct	", ",pacemaker
R1200	T2126	T2122	prep	of,domain
R1201	T2127	T2128	punct	~,700
R1202	T2128	T2129	nummod	700,residues
R1203	T2129	T2126	pobj	residues,of
R1204	T2130	T2129	punct	(,residues
R1205	T2131	T2132	punct	~,80
R1206	T2132	T2133	nummod	80,kDa
R1207	T2133	T2129	appos	kDa,residues
R1208	T2134	T2122	punct	),domain
R1209	T2135	T2122	punct	", ",domain
R121	T362	T363	compound	hexokinase,type
R1210	T2136	T2137	prep	for,identified
R1211	T2137	T2122	relcl	identified,domain
R1212	T2138	T2136	pobj	which,for
R1213	T2139	T2140	det	no,partners
R1214	T2140	T2137	nsubjpass	partners,identified
R1215	T2141	T2140	amod	molecular,partners
R1216	T2142	T2137	aux	have,identified
R1217	T2143	T2137	auxpass	been,identified
R1218	T2144	T2137	prep	until,identified
R1219	T2145	T2146	det	this,date
R122	T363	T358	appos	type,pacemaker
R1220	T2146	T2144	pobj	date,until
R1221	T2147	T2114	punct	.,is
R1222	T2149	T2150	nmod	Brain,libraries
R1223	T2150	T2157	nsubjpass	libraries,screened
R1224	T2151	T2149	cc	and,Brain
R1225	T2152	T2149	conj	retina,Brain
R1226	T2153	T2150	nmod	yeast,libraries
R1227	T2154	T2155	nummod	two,hybrid
R1228	T2155	T2150	compound	hybrid,libraries
R1229	T2156	T2155	punct	-,hybrid
R123	T364	T363	nummod	I,type
R1230	T2158	T2157	auxpass	were,screened
R1231	T2159	T2157	prep	with,screened
R1232	T2160	T2159	pobj	LD,with
R1233	T2161	T2157	punct	.,screened
R1234	T2163	T2164	nsubjpass	Cox11,identified
R1235	T2165	T2164	auxpass	was,identified
R1236	T2166	T2164	prep	as,identified
R1237	T2167	T2168	det	a,partner
R1238	T2168	T2166	pobj	partner,as
R1239	T2169	T2168	prep	to,partner
R124	T365	T363	punct	(,type
R1240	T2170	T2171	det	this,domain
R1241	T2171	T2169	pobj	domain,to
R1242	T2172	T2173	punct	(,1B
R1243	T2173	T2164	parataxis	1B,identified
R1244	T2174	T2173	compound	Figure,1B
R1245	T2175	T2173	cc	and,1B
R1246	T2176	T2173	conj	1C,1B
R1247	T2177	T2173	punct	),1B
R1248	T2178	T2164	punct	.,identified
R1249	T2180	T2181	nsubj	Cox11,is
R125	T366	T363	appos	HKI,type
R1250	T2182	T2183	det	a,metallochaperone
R1251	T2183	T2181	attr	metallochaperone,is
R1252	T2184	T2183	acl	implicated,metallochaperone
R1253	T2185	T2184	prep	in,implicated
R1254	T2186	T2187	compound	cytochrome,oxidase
R1255	T2187	T2189	compound	oxidase,assembly
R1256	T2188	T2187	compound	c,oxidase
R1257	T2189	T2185	pobj	assembly,in
R1258	T2190	T2191	punct	[,31
R1259	T2191	T2181	parataxis	31,is
R126	T367	T348	punct	),colocalizes
R1260	T2192	T2191	nummod	30,31
R1261	T2193	T2191	punct	",",31
R1262	T2194	T2191	punct	],31
R1263	T2195	T2181	punct	.,is
R1264	T2197	T2198	compound	Structure,function
R1265	T2198	T2200	compound	function,analysis
R1266	T2199	T2198	punct	-,function
R1267	T2200	T2201	nsubj	analysis,showed
R1268	T2202	T2200	prep	of,analysis
R1269	T2203	T2204	det	the,interaction
R127	T368	T348	prep	via,colocalizes
R1270	T2204	T2202	pobj	interaction,of
R1271	T2205	T2204	prep	between,interaction
R1272	T2206	T2205	pobj	mCox11,between
R1273	T2207	T2206	punct	", ",mCox11
R1274	T2208	T2206	conj	LD,mCox11
R1275	T2209	T2208	prep	of,LD
R1276	T2210	T2209	pobj	RanBP2,of
R1277	T2211	T2208	punct	", ",LD
R1278	T2212	T2208	cc	and,LD
R1279	T2213	T2208	conj	subdomains,LD
R128	T369	T370	poss	its,domain
R1280	T2214	T2213	advmod	thereof,subdomains
R1281	T2215	T2200	punct	", ",analysis
R1282	T2216	T2200	prep	with,analysis
R1283	T2217	T2218	amod	quantitative,assays
R1284	T2218	T2216	pobj	assays,with
R1285	T2219	T2218	nmod	yeast,assays
R1286	T2220	T2221	nummod	two,hybrid
R1287	T2221	T2218	compound	hybrid,assays
R1288	T2222	T2221	punct	-,hybrid
R1289	T2223	T2224	punct	[,32
R129	T370	T368	pobj	domain,via
R1290	T2224	T2200	parataxis	32,analysis
R1291	T2225	T2224	punct	],32
R1292	T2226	T2201	punct	", ",showed
R1293	T2227	T2228	amod	optimal,interaction
R1294	T2228	T2201	dobj	interaction,showed
R1295	T2229	T2228	prep	between,interaction
R1296	T2230	T2231	det	the,proteins
R1297	T2231	T2229	pobj	proteins,between
R1298	T2232	T2231	amod	intact,proteins
R1299	T2233	T2231	nmod	LD,proteins
R13	T242	T240	prep	of,Haploinsufficiency
R130	T371	T372	npadvmod	leucine,rich
R1300	T2234	T2233	cc	and,LD
R1301	T2235	T2233	conj	Cox11,LD
R1302	T2236	T2237	punct	(,1C
R1303	T2237	T2201	parataxis	1C,showed
R1304	T2238	T2237	compound	Figure,1C
R1305	T2239	T2237	punct	),1C
R1306	T2240	T2201	punct	.,showed
R1307	T2242	T2243	amod	Pull,assays
R1308	T2243	T2246	nsubj	assays,precipitates
R1309	T2244	T2242	punct	-,Pull
R131	T372	T370	amod	rich,domain
R1310	T2245	T2242	prt	down,Pull
R1311	T2247	T2243	prep	of,assays
R1312	T2248	T2249	amod	retinal,extracts
R1313	T2249	T2247	pobj	extracts,of
R1314	T2250	T2243	prep	with,assays
R1315	T2251	T2252	nmod	glutathione,transferase
R1316	T2252	T2255	nmod	transferase,LD
R1317	T2253	T2252	nmod	S,transferase
R1318	T2254	T2252	punct	-,transferase
R1319	T2255	T2250	pobj	LD,with
R132	T373	T372	punct	-,rich
R1320	T2256	T2252	punct	(,transferase
R1321	T2257	T2252	appos	GST,transferase
R1322	T2258	T2255	punct	),LD
R1323	T2259	T2255	punct	-,LD
R1324	T2260	T2261	det	a,isoform
R1325	T2261	T2246	dobj	isoform,precipitates
R1326	T2262	T2263	compound	sodium,sulfate
R1327	T2263	T2265	npadvmod	sulfate,resistant
R1328	T2264	T2263	compound	dodecyl,sulfate
R1329	T2265	T2261	amod	resistant,isoform
R133	T374	T341	punct	.,associates
R1330	T2266	T2265	punct	-,resistant
R1331	T2267	T2261	compound	dimer,isoform
R1332	T2268	T2261	prep	of,isoform
R1333	T2269	T2268	pobj	Cox11,of
R1334	T2270	T2271	punct	(,panel
R1335	T2271	T2261	parataxis	panel,isoform
R1336	T2272	T2273	compound	Figure,1D
R1337	T2273	T2271	dep	1D,panel
R1338	T2274	T2271	punct	", ",panel
R1339	T2275	T2271	amod	top,panel
R134	T376	T377	det	The,domain
R1340	T2276	T2271	punct	),panel
R1341	T2277	T2261	punct	", ",isoform
R1342	T2278	T2279	dep	which,bind
R1343	T2279	T2261	relcl	bind,isoform
R1344	T2280	T2279	aux	does,bind
R1345	T2281	T2279	neg	not,bind
R1346	T2282	T2279	prep	to,bind
R1347	T2283	T2284	compound	GST,LDZIP
R1348	T2284	T2282	pobj	LDZIP,to
R1349	T2285	T2284	punct	-,LDZIP
R135	T377	T381	nsubj	domain,exhibits
R1350	T2286	T2284	advmod	alone,LDZIP
R1351	T2287	T2246	punct	.,precipitates
R1352	T2289	T2290	prep	In,found
R1353	T2291	T2289	pobj	addition,In
R1354	T2292	T2291	prep	to,addition
R1355	T2293	T2292	pobj	Cox11,to
R1356	T2294	T2290	punct	", ",found
R1357	T2295	T2290	nsubj	we,found
R1358	T2296	T2290	advmod	also,found
R1359	T2297	T2298	mark	that,associated
R136	T378	T379	npadvmod	leucine,rich
R1360	T2298	T2290	ccomp	associated,found
R1361	T2299	T2300	amod	other,components
R1362	T2300	T2298	nsubj	components,associated
R1363	T2301	T2300	amod	mitochondrial,components
R1364	T2302	T2303	amod	such,as
R1365	T2303	T2300	prep	as,components
R1366	T2304	T2305	det	the,protein
R1367	T2305	T2303	pobj	protein,as
R1368	T2306	T2307	amod	outer,membrane
R1369	T2307	T2308	npadvmod	membrane,associated
R137	T379	T377	amod	rich,domain
R1370	T2308	T2305	amod	associated,protein
R1371	T2309	T2308	punct	-,associated
R1372	T2310	T2305	punct	", ",protein
R1373	T2311	T2305	appos	HKI,protein
R1374	T2312	T2313	punct	[,33
R1375	T2313	T2311	parataxis	33,HKI
R1376	T2314	T2313	punct	],33
R1377	T2315	T2316	punct	(,panel
R1378	T2316	T2311	parataxis	panel,HKI
R1379	T2317	T2318	compound	Figure,1D
R138	T380	T379	punct	-,rich
R1380	T2318	T2316	dep	1D,panel
R1381	T2319	T2316	punct	", ",panel
R1382	T2320	T2316	amod	bottom,panel
R1383	T2321	T2316	punct	),panel
R1384	T2322	T2311	cc	and,HKI
R1385	T2323	T2311	conj	mHsp70,HKI
R1386	T2324	T2325	punct	(,data
R1387	T2325	T2323	meta	data,mHsp70
R1388	T2326	T2325	amod	unpublished,data
R1389	T2327	T2325	punct	),data
R139	T382	T377	prep	of,domain
R1390	T2328	T2298	punct	", ",associated
R1391	T2329	T2298	prep	with,associated
R1392	T2330	T2329	pobj	LD,with
R1393	T2331	T2330	prep	of,LD
R1394	T2332	T2331	pobj	RanBP2,of
R1395	T2333	T2290	punct	.,found
R1396	T2335	T2336	det	This,association
R1397	T2336	T2337	nsubj	association,was
R1398	T2338	T2339	advmod	highly,specific
R1399	T2339	T2337	acomp	specific,was
R14	T243	T242	pobj	RanBP2,of
R140	T383	T382	pobj	RanBP2,of
R1400	T2340	T2339	prep	toward,specific
R1401	T2341	T2342	det	the,isoform
R1402	T2342	T2340	pobj	isoform,toward
R1403	T2343	T2342	compound	HKI,isoform
R1404	T2344	T2337	punct	", ",was
R1405	T2345	T2346	mark	because,interact
R1406	T2346	T2337	advcl	interact,was
R1407	T2347	T2346	nsubj	HKII,interact
R1408	T2348	T2347	punct	", ",HKII
R1409	T2349	T2347	conj	HKIII,HKII
R141	T384	T381	advmod	also,exhibits
R1410	T2350	T2349	punct	", ",HKIII
R1411	T2351	T2349	cc	and,HKIII
R1412	T2352	T2349	conj	glucokinase,HKIII
R1413	T2353	T2346	aux	did,interact
R1414	T2354	T2346	neg	not,interact
R1415	T2355	T2346	prep	with,interact
R1416	T2356	T2357	det	the,LD
R1417	T2357	T2355	pobj	LD,with
R1418	T2358	T2357	prep	of,LD
R1419	T2359	T2358	pobj	RanBP2,of
R142	T385	T386	amod	strong,activity
R1420	T2360	T2361	punct	(,data
R1421	T2361	T2346	meta	data,interact
R1422	T2362	T2361	amod	unpublished,data
R1423	T2363	T2361	punct	),data
R1424	T2364	T2337	punct	.,was
R1425	T2366	T2367	det	The,interaction
R1426	T2367	T2368	nsubj	interaction,occurred
R1427	T2369	T2367	prep	of,interaction
R1428	T2370	T2369	pobj	Cox11,of
R1429	T2371	T2370	punct	", ",Cox11
R143	T386	T381	dobj	activity,exhibits
R1430	T2372	T2370	conj	HKI,Cox11
R1431	T2373	T2372	punct	", ",HKI
R1432	T2374	T2372	cc	and,HKI
R1433	T2375	T2372	conj	mHsp70,HKI
R1434	T2376	T2367	prep	with,interaction
R1435	T2377	T2376	pobj	RanBP2,with
R1436	T2378	T2379	advmod	in,vivo
R1437	T2379	T2368	advmod	vivo,occurred
R1438	T2380	T2368	prep	in,occurred
R1439	T2381	T2382	amod	retinal,extracts
R144	T387	T386	compound	chaperone,activity
R1440	T2382	T2380	pobj	extracts,in
R1441	T2383	T2368	punct	", ",occurred
R1442	T2384	T2385	mark	since,coimmunoprecipitated
R1443	T2385	T2368	advcl	coimmunoprecipitated,occurred
R1444	T2386	T2385	nsubj	antibodies,coimmunoprecipitated
R1445	T2387	T2386	prep	against,antibodies
R1446	T2388	T2389	det	these,RanBP2
R1447	T2389	T2387	pobj	RanBP2,against
R1448	T2390	T2389	cc	and,RanBP2
R1449	T2391	T2385	dobj	RanBP2,coimmunoprecipitated
R145	T388	T386	prep	toward,activity
R1450	T2392	T2393	punct	(,1E
R1451	T2393	T2391	parataxis	1E,RanBP2
R1452	T2394	T2393	compound	Figure,1E
R1453	T2395	T2393	punct	),1E
R1454	T2396	T2391	cc	and,RanBP2
R1455	T2397	T2391	conj	HKI,RanBP2
R1456	T2398	T2399	punct	(,1F
R1457	T2399	T2397	parataxis	1F,HKI
R1458	T2400	T2399	compound	Figure,1F
R1459	T2401	T2399	punct	),1F
R146	T389	T390	amod	intermediate,species
R1460	T2402	T2385	punct	", ",coimmunoprecipitated
R1461	T2403	T2385	advmod	respectively,coimmunoprecipitated
R1462	T2404	T2385	punct	", ",coimmunoprecipitated
R1463	T2405	T2385	cc	and,coimmunoprecipitated
R1464	T2406	T2407	det	these,interactions
R1465	T2407	T2408	nsubjpass	interactions,observed
R1466	T2408	T2385	conj	observed,coimmunoprecipitated
R1467	T2409	T2408	auxpass	were,observed
R1468	T2410	T2408	prep	across,observed
R1469	T2411	T2412	amod	different,tissues
R147	T390	T388	pobj	species,toward
R1470	T2412	T2410	pobj	tissues,across
R1471	T2413	T2414	punct	(,S1
R1472	T2414	T2408	parataxis	S1,observed
R1473	T2415	T2414	compound	Figure,S1
R1474	T2416	T2414	punct	),S1
R1475	T2417	T2368	punct	.,occurred
R1476	T2419	T2420	mark	Since,exhibits
R1477	T2420	T2422	advcl	exhibits,assessed
R1478	T2421	T2420	nsubj	RanBP2,exhibits
R1479	T2423	T2424	compound	chaperone,activity
R148	T391	T389	cc	and,intermediate
R1480	T2424	T2420	dobj	activity,exhibits
R1481	T2425	T2422	punct	", ",assessed
R1482	T2426	T2422	nsubj	we,assessed
R1483	T2427	T2428	mark	whether,was
R1484	T2428	T2422	ccomp	was,assessed
R1485	T2429	T2430	det	the,interaction
R1486	T2430	T2428	nsubj	interaction,was
R1487	T2431	T2430	prep	between,interaction
R1488	T2432	T2433	det	the,LD
R1489	T2433	T2431	pobj	LD,between
R149	T392	T389	conj	mature,intermediate
R1490	T2434	T2433	prep	of,LD
R1491	T2435	T2434	pobj	RanBP2,of
R1492	T2436	T2433	cc	and,LD
R1493	T2437	T2433	conj	Cox11,LD
R1494	T2438	T2428	acomp	direct,was
R1495	T2439	T2440	cc	and,activity
R1496	T2440	T2428	dep	activity,was
R1497	T2441	T2440	det	the,activity
R1498	T2442	T2440	compound	chaperone,activity
R1499	T2443	T2440	prep	of,activity
R15	T244	T241	dobj	Deficits,Causes
R150	T393	T390	compound	folding,species
R1500	T2444	T2443	pobj	LD,of
R1501	T2445	T2440	prep	toward,activity
R1502	T2446	T2447	amod	folding,species
R1503	T2447	T2445	pobj	species,toward
R1504	T2448	T2447	prep	of,species
R1505	T2449	T2448	pobj	Cox11,of
R1506	T2450	T2422	punct	.,assessed
R1507	T2452	T2453	compound	Reconstitution,binding
R1508	T2453	T2454	compound	binding,assays
R1509	T2454	T2455	nsubjpass	assays,carried
R151	T394	T390	prep	of,species
R1510	T2456	T2455	auxpass	were,carried
R1511	T2457	T2455	prt	out,carried
R1512	T2458	T2455	prep	between,carried
R1513	T2459	T2460	amod	purified,LD
R1514	T2460	T2458	pobj	LD,between
R1515	T2461	T2460	cc	and,LD
R1516	T2462	T2460	conj	Cox11,LD
R1517	T2463	T2462	punct	", ",Cox11
R1518	T2464	T2465	advmod	fully,denatured
R1519	T2465	T2462	acl	denatured,Cox11
R152	T395	T394	pobj	Cox11,of
R1520	T2466	T2464	cc	and,fully
R1521	T2467	T2464	conj	partially,fully
R1522	T2468	T2465	prep	with,denatured
R1523	T2469	T2468	pobj	GnHCl,with
R1524	T2470	T2469	cc	and,GnHCl
R1525	T2471	T2469	conj	urea,GnHCl
R1526	T2472	T2465	punct	", ",denatured
R1527	T2473	T2465	advmod	respectively,denatured
R1528	T2474	T2462	punct	", ",Cox11
R1529	T2475	T2462	cc	and,Cox11
R153	T396	T381	advcl	supporting,exhibits
R1530	T2476	T2477	amod	native,Cox11
R1531	T2477	T2462	conj	Cox11,Cox11
R1532	T2478	T2479	punct	(,1G
R1533	T2479	T2477	parataxis	1G,Cox11
R1534	T2480	T2479	compound	Figure,1G
R1535	T2481	T2479	cc	and,1G
R1536	T2482	T2479	conj	1H,1G
R1537	T2483	T2479	punct	),1G
R1538	T2484	T2455	punct	.,carried
R1539	T2486	T2487	amod	Partial,denatured
R154	T397	T398	det	a,role
R1540	T2487	T2488	amod	denatured,Cox11
R1541	T2488	T2489	nsubj	Cox11,exhibits
R1542	T2490	T2491	advmod	significantly,higher
R1543	T2491	T2492	amod	higher,affinity
R1544	T2492	T2489	dobj	affinity,exhibits
R1545	T2493	T2491	cc	and,higher
R1546	T2494	T2495	npadvmod	concentration,dependent
R1547	T2495	T2491	conj	dependent,higher
R1548	T2496	T2495	punct	-,dependent
R1549	T2497	T2492	compound	binding,affinity
R155	T398	T396	dobj	role,supporting
R1550	T2498	T2492	prep	toward,affinity
R1551	T2499	T2498	pobj	LD,toward
R1552	T2500	T2489	prep	compared,exhibits
R1553	T2501	T2500	prep	with,compared
R1554	T2502	T2503	det	the,Cox11
R1555	T2503	T2501	pobj	Cox11,with
R1556	T2504	T2503	amod	native,Cox11
R1557	T2505	T2504	cc	and,native
R1558	T2506	T2507	advmod	fully,denatured
R1559	T2507	T2504	conj	denatured,native
R156	T399	T398	compound	chaperone,role
R1560	T2508	T2509	punct	(,1G
R1561	T2509	T2489	parataxis	1G,exhibits
R1562	T2510	T2509	compound	Figure,1G
R1563	T2511	T2509	punct	),1G
R1564	T2512	T2489	punct	.,exhibits
R1565	T2514	T2515	prep	In,shows
R1566	T2516	T2514	pobj	addition,In
R1567	T2517	T2515	punct	", ",shows
R1568	T2518	T2519	amod	native,Cox11
R1569	T2519	T2515	nsubj	Cox11,shows
R157	T400	T398	prep	of,role
R1570	T2520	T2519	acl	purified,Cox11
R1571	T2521	T2520	prep	upon,purified
R1572	T2522	T2521	pobj	expression,upon
R1573	T2523	T2520	prep	in,purified
R1574	T2524	T2525	det	the,presence
R1575	T2525	T2523	pobj	presence,in
R1576	T2526	T2525	prep	of,presence
R1577	T2527	T2526	pobj	CuSO4,of
R1578	T2528	T2527	punct	(,CuSO4
R1579	T2529	T2530	det	a,group
R158	T401	T400	pobj	RanBP2,of
R1580	T2530	T2527	appos	group,CuSO4
R1581	T2531	T2530	amod	prosthetic,group
R1582	T2532	T2533	advmod	tightly,bound
R1583	T2533	T2530	acl	bound,group
R1584	T2534	T2533	prep	to,bound
R1585	T2535	T2534	pobj	Cox11,to
R1586	T2536	T2520	punct	),purified
R1587	T2537	T2538	punct	[,31
R1588	T2538	T2520	parataxis	31,purified
R1589	T2539	T2538	punct	],31
R159	T402	T398	prep	in,role
R1590	T2540	T2515	punct	", ",shows
R1591	T2541	T2542	advmod	significantly,higher
R1592	T2542	T2543	amod	higher,activity
R1593	T2543	T2515	dobj	activity,shows
R1594	T2544	T2543	compound	binding,activity
R1595	T2545	T2543	prep	toward,activity
R1596	T2546	T2547	det	the,LD
R1597	T2547	T2545	pobj	LD,toward
R1598	T2548	T2547	prep	of,LD
R1599	T2549	T2548	pobj	RanBP2,of
R16	T245	T244	prep	in,Deficits
R160	T403	T404	det	the,cytosol
R1600	T2550	T2543	punct	", ",activity
R1601	T2551	T2543	prep	than,activity
R1602	T2552	T2551	prep	in,than
R1603	T2553	T2554	det	the,absence
R1604	T2554	T2552	pobj	absence,in
R1605	T2555	T2554	prep	of,absence
R1606	T2556	T2555	pobj	CuSO4,of
R1607	T2557	T2558	punct	(,1H
R1608	T2558	T2515	parataxis	1H,shows
R1609	T2559	T2558	compound	Figure,1H
R161	T404	T402	pobj	cytosol,in
R1610	T2560	T2558	punct	),1H
R1611	T2561	T2515	punct	.,shows
R1612	T2637	T2638	nsubj	Cox,Inhibits
R1613	T2639	T2637	nummod	11,Cox
R1614	T2640	T2641	compound	HKI,Activity
R1615	T2641	T2638	dobj	Activity,Inhibits
R1616	T2642	T2641	cc	and,Activity
R1617	T2643	T2644	det	the,LD
R1618	T2644	T2641	conj	LD,Activity
R1619	T2645	T2644	prep	of,LD
R162	T405	T398	prep	during,role
R1620	T2646	T2645	pobj	RanBP2,of
R1621	T2647	T2638	dep	Reverses,Inhibits
R1622	T2648	T2649	det	the,Inhibition
R1623	T2649	T2647	dobj	Inhibition,Reverses
R1624	T2650	T2649	prep	of,Inhibition
R1625	T2651	T2650	pobj	Cox11,of
R1626	T2652	T2649	prep	over,Inhibition
R1627	T2653	T2652	pobj	HKI,over
R1628	T2655	T2656	aux	To,probe
R1629	T2656	T2657	advcl	probe,examined
R163	T406	T407	compound	Cox11,biogenesis
R1630	T2658	T2659	mark	whether,modulates
R1631	T2659	T2656	ccomp	modulates,probe
R1632	T2660	T2661	det	the,interaction
R1633	T2661	T2659	nsubj	interaction,modulates
R1634	T2662	T2661	prep	of,interaction
R1635	T2663	T2662	pobj	Cox11,of
R1636	T2664	T2663	cc	and,Cox11
R1637	T2665	T2663	conj	HKI,Cox11
R1638	T2666	T2661	prep	with,interaction
R1639	T2667	T2668	det	the,LD
R164	T407	T405	pobj	biogenesis,during
R1640	T2668	T2666	pobj	LD,with
R1641	T2669	T2668	prep	of,LD
R1642	T2670	T2669	pobj	RanBP2,of
R1643	T2671	T2672	det	the,activity
R1644	T2672	T2659	dobj	activity,modulates
R1645	T2673	T2672	amod	enzymatic,activity
R1646	T2674	T2672	prep	of,activity
R1647	T2675	T2674	pobj	HKI,of
R1648	T2676	T2657	punct	", ",examined
R1649	T2677	T2657	nsubj	we,examined
R165	T408	T381	punct	.,exhibits
R1650	T2678	T2657	advmod	first,examined
R1651	T2679	T2680	det	the,effect
R1652	T2680	T2657	dobj	effect,examined
R1653	T2681	T2680	prep	of,effect
R1654	T2682	T2681	pcomp	increasing,of
R1655	T2683	T2682	dobj	concentrations,increasing
R1656	T2684	T2683	prep	of,concentrations
R1657	T2685	T2684	pobj	Cox11,of
R1658	T2686	T2680	prep	on,effect
R1659	T2687	T2688	det	the,rates
R166	T410	T411	nsubj	Cox11,colocalizes
R1660	T2688	T2686	pobj	rates,on
R1661	T2689	T2688	amod	initial,rates
R1662	T2690	T2688	prep	of,rates
R1663	T2691	T2692	nmod	HKI,activity
R1664	T2692	T2690	pobj	activity,of
R1665	T2693	T2692	amod	enzymatic,activity
R1666	T2694	T2695	punct	(,2A
R1667	T2695	T2657	parataxis	2A,examined
R1668	T2696	T2695	compound	Figure,2A
R1669	T2697	T2695	punct	),2A
R167	T412	T411	advmod	partially,colocalizes
R1670	T2698	T2657	punct	.,examined
R1671	T2700	T2701	nsubj	Cox11,inhibits
R1672	T2702	T2701	advmod	strongly,inhibits
R1673	T2703	T2704	compound	HKI,activity
R1674	T2704	T2701	dobj	activity,inhibits
R1675	T2705	T2701	prep	in,inhibits
R1676	T2706	T2707	det	a,fashion
R1677	T2707	T2705	pobj	fashion,in
R1678	T2708	T2709	npadvmod	concentration,dependent
R1679	T2709	T2707	amod	dependent,fashion
R168	T413	T411	prep	with,colocalizes
R1680	T2710	T2709	punct	-,dependent
R1681	T2711	T2701	punct	", ",inhibits
R1682	T2712	T2701	cc	and,inhibits
R1683	T2713	T2714	prep	at,recorded
R1684	T2714	T2701	conj	recorded,inhibits
R1685	T2715	T2716	punct	~,15
R1686	T2716	T2717	nummod	15,nM
R1687	T2717	T2713	pobj	nM,at
R1688	T2718	T2717	prep	of,nM
R1689	T2719	T2718	pobj	Cox11,of
R169	T414	T413	pobj	HKI,with
R1690	T2720	T2714	punct	", ",recorded
R1691	T2721	T2722	compound	HKI,activity
R1692	T2722	T2714	nsubjpass	activity,recorded
R1693	T2723	T2714	aux	could,recorded
R1694	T2724	T2714	neg	not,recorded
R1695	T2725	T2714	auxpass	be,recorded
R1696	T2726	T2727	punct	(,2A
R1697	T2727	T2714	parataxis	2A,recorded
R1698	T2728	T2727	compound	Figure,2A
R1699	T2729	T2727	punct	),2A
R17	T246	T247	compound	Glucose,Metabolism
R170	T415	T411	punct	", ",colocalizes
R1700	T2730	T2714	punct	.,recorded
R1701	T2732	T2733	nsubj	Cox11,behaves
R1702	T2734	T2733	prep	as,behaves
R1703	T2735	T2736	det	a,inhibitor
R1704	T2736	T2734	pobj	inhibitor,as
R1705	T2737	T2736	amod	partial,inhibitor
R1706	T2738	T2736	amod	noncompetitive,inhibitor
R1707	T2739	T2736	prep	of,inhibitor
R1708	T2740	T2739	pobj	HKI,of
R1709	T2741	T2733	prep	by,behaves
R171	T416	T417	advmod	thus,supporting
R1710	T2742	T2741	pcomp	affecting,by
R1711	T2743	T2744	det	the,V max
R1712	T2744	T2742	dobj	V max,affecting
R1713	T2745	T2744	prep	of,V max
R1714	T2746	T2745	pobj	HKI,of
R1715	T2747	T2744	prep	for,V max
R1716	T2748	T2747	pobj	glucose,for
R1717	T2749	T2750	punct	(,2B
R1718	T2750	T2742	parataxis	2B,affecting
R1719	T2751	T2750	compound	Figure,2B
R172	T417	T411	advcl	supporting,colocalizes
R1720	T2752	T2750	punct	),2B
R1721	T2753	T2733	punct	.,behaves
R1722	T2755	T2756	advmod	Then,evaluated
R1723	T2757	T2756	punct	", ",evaluated
R1724	T2758	T2756	nsubj	we,evaluated
R1725	T2759	T2760	det	the,effect
R1726	T2760	T2756	dobj	effect,evaluated
R1727	T2761	T2760	prep	of,effect
R1728	T2762	T2763	det	the,LD
R1729	T2763	T2761	pobj	LD,of
R173	T418	T419	amod	additional,roles
R1730	T2764	T2763	prep	of,LD
R1731	T2765	T2764	pobj	RanBP2,of
R1732	T2766	T2760	prep	on,effect
R1733	T2767	T2768	det	the,activity
R1734	T2768	T2766	pobj	activity,on
R1735	T2769	T2768	compound	HK,activity
R1736	T2770	T2756	prep	in,evaluated
R1737	T2771	T2772	det	the,presence
R1738	T2772	T2770	pobj	presence,in
R1739	T2773	T2772	prep	of,presence
R174	T419	T417	dobj	roles,supporting
R1740	T2774	T2775	det	a,concentration
R1741	T2775	T2773	pobj	concentration,of
R1742	T2776	T2775	amod	fixed,concentration
R1743	T2777	T2775	amod	inhibitory,concentration
R1744	T2778	T2775	prep	of,concentration
R1745	T2779	T2778	pobj	Cox11,of
R1746	T2780	T2775	punct	", ",concentration
R1747	T2781	T2782	amod	saturating,concentration
R1748	T2782	T2775	conj	concentration,concentration
R1749	T2783	T2782	prep	of,concentration
R175	T420	T418	cc	and,additional
R1750	T2784	T2785	compound	glucose,substrate
R1751	T2785	T2783	pobj	substrate,of
R1752	T2786	T2782	punct	", ",concentration
R1753	T2787	T2782	cc	and,concentration
R1754	T2788	T2789	amod	increasing,concentrations
R1755	T2789	T2782	conj	concentrations,concentration
R1756	T2790	T2789	prep	of,concentrations
R1757	T2791	T2790	pobj	LD,of
R1758	T2792	T2756	punct	.,evaluated
R1759	T2794	T2795	mark	As,shown
R176	T421	T418	conj	distinct,additional
R1760	T2795	T2796	advcl	shown,reversed
R1761	T2797	T2795	prep	in,shown
R1762	T2798	T2799	compound	Figure,2C
R1763	T2799	T2797	pobj	2C,in
R1764	T2800	T2796	punct	", ",reversed
R1765	T2801	T2802	det	the,domain
R1766	T2802	T2796	nsubj	domain,reversed
R1767	T2803	T2802	compound	LD,domain
R1768	T2804	T2796	advmod	sharply,reversed
R1769	T2805	T2806	det	the,effect
R177	T422	T419	prep	in,roles
R1770	T2806	T2796	dobj	effect,reversed
R1771	T2807	T2806	amod	inhibitory,effect
R1772	T2808	T2806	prep	of,effect
R1773	T2809	T2808	pobj	Cox11,of
R1774	T2810	T2806	prep	on,effect
R1775	T2811	T2812	compound	HKI,activity
R1776	T2812	T2810	pobj	activity,on
R1777	T2813	T2796	prep	in,reversed
R1778	T2814	T2815	det	a,manner
R1779	T2815	T2813	pobj	manner,in
R178	T423	T424	compound	cell,function
R1780	T2816	T2817	npadvmod	concentration,dependent
R1781	T2817	T2815	amod	dependent,manner
R1782	T2818	T2817	punct	-,dependent
R1783	T2819	T2796	punct	", ",reversed
R1784	T2820	T2796	cc	but,reversed
R1785	T2821	T2822	prep	under,reach
R1786	T2822	T2796	conj	reach,reversed
R1787	T2823	T2824	amod	saturating,amounts
R1788	T2824	T2821	pobj	amounts,under
R1789	T2825	T2823	punct	(,saturating
R179	T424	T422	pobj	function,in
R1790	T2826	T2823	cc	and,saturating
R1791	T2827	T2823	conj	stochiometric,saturating
R1792	T2828	T2824	punct	),amounts
R1793	T2829	T2824	prep	of,amounts
R1794	T2830	T2829	pobj	LD,of
R1795	T2831	T2822	punct	", ",reach
R1796	T2832	T2833	det	the,velocity
R1797	T2833	T2822	nsubj	velocity,reach
R1798	T2834	T2833	prep	of,velocity
R1799	T2835	T2836	det	the,reaction
R18	T247	T245	pobj	Metabolism,in
R180	T425	T411	punct	.,colocalizes
R1800	T2836	T2834	pobj	reaction,of
R1801	T2837	T2822	aux	did,reach
R1802	T2838	T2822	neg	not,reach
R1803	T2839	T2822	dobj	that,reach
R1804	T2840	T2839	acl	observed,that
R1805	T2841	T2840	prep	for,observed
R1806	T2842	T2843	compound	HKI,activity
R1807	T2843	T2841	pobj	activity,for
R1808	T2844	T2840	prep	in,observed
R1809	T2845	T2846	det	the,absence
R181	T427	T428	nsubj	Cox11,is
R1810	T2846	T2844	pobj	absence,in
R1811	T2847	T2846	prep	of,absence
R1812	T2848	T2847	pobj	Cox11,of
R1813	T2849	T2850	punct	(,2A
R1814	T2850	T2840	parataxis	2A,observed
R1815	T2851	T2850	compound	Figure,2A
R1816	T2852	T2850	punct	),2A
R1817	T2853	T2822	punct	", ",reach
R1818	T2854	T2822	advcl	suggesting,reach
R1819	T2855	T2856	det	the,LD
R182	T429	T430	det	a,inhibitor
R1820	T2856	T2857	nsubj	LD,have
R1821	T2857	T2854	advcl	have,suggesting
R1822	T2858	T2857	prep	by,have
R1823	T2859	T2858	pobj	itself,by
R1824	T2860	T2857	aux	may,have
R1825	T2861	T2857	advmod	also,have
R1826	T2862	T2863	det	an,effect
R1827	T2863	T2857	dobj	effect,have
R1828	T2864	T2863	prep	on,effect
R1829	T2865	T2866	compound	HKI,activity
R183	T430	T428	attr	inhibitor,is
R1830	T2866	T2864	pobj	activity,on
R1831	T2867	T2822	punct	.,reach
R1832	T2869	T2870	advmod	Indeed,reduced
R1833	T2871	T2870	punct	", ",reduced
R1834	T2872	T2873	det	a,concentration
R1835	T2873	T2870	nsubj	concentration,reduced
R1836	T2874	T2873	compound	saturating,concentration
R1837	T2875	T2873	prep	of,concentration
R1838	T2876	T2875	pobj	LD,of
R1839	T2877	T2878	det	the,V max
R184	T431	T430	amod	strong,inhibitor
R1840	T2878	T2870	dobj	V max,reduced
R1841	T2879	T2878	cc	but,V max
R1842	T2880	T2879	neg	not,but
R1843	T2881	T2882	det	the,K m
R1844	T2882	T2878	conj	K m,V max
R1845	T2883	T2878	prep	of,V max
R1846	T2884	T2883	pobj	HKI,of
R1847	T2885	T2886	punct	(,2D
R1848	T2886	T2878	parataxis	2D,V max
R1849	T2887	T2886	compound	Figure,2D
R185	T432	T430	prep	of,inhibitor
R1850	T2888	T2886	punct	),2D
R1851	T2889	T2870	prep	by,reduced
R1852	T2890	T2891	punct	~,20
R1853	T2891	T2892	nummod	20,%
R1854	T2892	T2889	pobj	%,by
R1855	T2893	T2870	prep	under,reduced
R1856	T2894	T2895	amod	similar,conditions
R1857	T2895	T2893	pobj	conditions,under
R1858	T2896	T2870	punct	.,reduced
R186	T433	T432	pobj	HKI,of
R1865	T2996	T2997	nsubj	RanBP2,Colocalizes
R1866	T2998	T2997	prep	with,Colocalizes
R1867	T2999	T2998	pobj	HK1,with
R1868	T3000	T2999	punct	", ",HK1
R1869	T3001	T2999	conj	Cox11,HK1
R187	T434	T428	punct	", ",is
R1870	T3002	T3001	punct	", ",Cox11
R1871	T3003	T3001	cc	and,Cox11
R1872	T3004	T3001	conj	mHsp70,Cox11
R1873	T3005	T2997	prep	in,Colocalizes
R1874	T3006	T3007	det	the,Retina
R1875	T3007	T3005	pobj	Retina,in
R1876	T3008	T3007	cc	and,Retina
R1877	T3009	T3010	amod	Cultured,Neurons
R1878	T3010	T3007	conj	Neurons,Retina
R1879	T3012	T3013	prep	In,shown
R188	T435	T428	cc	and,is
R1880	T3014	T3012	pobj	addition,In
R1881	T3015	T3014	prep	to,addition
R1882	T3016	T3017	poss	its,presence
R1883	T3017	T3015	pobj	presence,to
R1884	T3018	T3017	prep	at,presence
R1885	T3019	T3020	det	the,vicinity
R1886	T3020	T3018	pobj	vicinity,at
R1887	T3021	T3020	prep	of,vicinity
R1888	T3022	T3021	pobj	NPCs,of
R1889	T3023	T3013	punct	", ",shown
R189	T436	T437	nsubj	RanBP2,suppresses
R1890	T3024	T3013	nsubj	we,shown
R1891	T3025	T3013	aux	have,shown
R1892	T3026	T3013	advmod	previously,shown
R1893	T3027	T3028	mark	that,is
R1894	T3028	T3013	ccomp	is,shown
R1895	T3029	T3028	nsubj	RanBP2,is
R1896	T3030	T3028	dep	localized,is
R1897	T3031	T3030	prep	to,localized
R1898	T3032	T3030	cc	and,localized
R1899	T3033	T3034	amod	abundant,compartment
R19	T249	T250	det	The,protein
R190	T437	T428	conj	suppresses,is
R1900	T3034	T3030	conj	compartment,localized
R1901	T3035	T3033	prep	in,abundant
R1902	T3036	T3034	det	the,compartment
R1903	T3037	T3038	npadvmod	mitochondria,rich
R1904	T3038	T3034	amod	rich,compartment
R1905	T3039	T3038	punct	-,rich
R1906	T3040	T3034	amod	ellipsoid,compartment
R1907	T3041	T3034	amod	subcellular,compartment
R1908	T3042	T3034	prep	of,compartment
R1909	T3043	T3044	amod	photosensory,neurons
R191	T438	T439	det	the,activity
R1910	T3044	T3042	pobj	neurons,of
R1911	T3045	T3046	punct	(,photoreceptor
R1912	T3046	T3043	parataxis	photoreceptor,photosensory
R1913	T3047	T3046	punct	),photoreceptor
R1914	T3048	T3044	prep	of,neurons
R1915	T3049	T3050	det	the,retina
R1916	T3050	T3048	pobj	retina,of
R1917	T3051	T3052	punct	[,19
R1918	T3052	T3028	parataxis	19,is
R1919	T3053	T3052	punct	],19
R192	T439	T437	dobj	activity,suppresses
R1920	T3054	T3013	punct	.,shown
R1921	T3056	T3057	nsubj	We,extended
R1922	T3058	T3059	det	the,studies
R1923	T3059	T3057	dobj	studies,extended
R1924	T3060	T3059	amod	subcellular,studies
R1925	T3061	T3059	compound	colocalization,studies
R1926	T3062	T3059	prep	on,studies
R1927	T3063	T3062	pobj	RanBP2,on
R1928	T3064	T3063	cc	and,RanBP2
R1929	T3065	T3066	poss	its,partners
R193	T440	T439	amod	inhibitory,activity
R1930	T3066	T3063	conj	partners,RanBP2
R1931	T3067	T3066	amod	novel,partners
R1932	T3068	T3057	prep	by,extended
R1933	T3069	T3068	pobj	immunocytochemistry,by
R1934	T3070	T3071	aux	to,determine
R1935	T3071	T3057	advcl	determine,extended
R1936	T3072	T3073	mark	if,colocalize
R1937	T3073	T3071	ccomp	colocalize,determine
R1938	T3074	T3073	nsubj	RanBP2,colocalize
R1939	T3075	T3074	punct	", ",RanBP2
R194	T441	T439	prep	of,activity
R1940	T3076	T3074	conj	Cox11,RanBP2
R1941	T3077	T3076	punct	", ",Cox11
R1942	T3078	T3076	conj	HKI,Cox11
R1943	T3079	T3078	punct	", ",HKI
R1944	T3080	T3078	cc	and,HKI
R1945	T3081	T3078	conj	mHsp70,HKI
R1946	T3082	T3073	prep	in,colocalize
R1947	T3083	T3084	amod	hippocampal,neurons
R1948	T3084	T3082	pobj	neurons,in
R1949	T3085	T3086	punct	(,3A
R195	T442	T441	pobj	Cox11,of
R1950	T3086	T3084	parataxis	3A,neurons
R1951	T3087	T3086	compound	Figure,3A
R1952	T3088	T3089	punct	–,3C
R1953	T3089	T3086	prep	3C,3A
R1954	T3090	T3086	punct	),3A
R1955	T3091	T3084	punct	", ",neurons
R1956	T3092	T3093	amod	cerebral,cortex
R1957	T3093	T3094	compound	cortex,neurons
R1958	T3094	T3084	conj	neurons,neurons
R1959	T3095	T3096	punct	(,3D
R196	T443	T439	prep	over,activity
R1960	T3096	T3094	parataxis	3D,neurons
R1961	T3097	T3096	compound	Figure,3D
R1962	T3098	T3099	punct	–,3F
R1963	T3099	T3096	prep	3F,3D
R1964	T3100	T3096	punct	),3D
R1965	T3101	T3094	punct	", ",neurons
R1966	T3102	T3103	amod	ellipsoid,compartments
R1967	T3103	T3094	conj	compartments,neurons
R1968	T3104	T3103	punct	(,compartments
R1969	T3105	T3106	npadvmod	mitochondria,rich
R197	T444	T443	pobj	HKI,over
R1970	T3106	T3103	amod	rich,compartments
R1971	T3107	T3106	punct	-,rich
R1972	T3108	T3103	punct	),compartments
R1973	T3109	T3103	amod	subcellular,compartments
R1974	T3110	T3103	prep	of,compartments
R1975	T3111	T3112	amod	photosensory,neurons
R1976	T3112	T3110	pobj	neurons,of
R1977	T3113	T3112	prep	of,neurons
R1978	T3114	T3115	det	the,retina
R1979	T3115	T3113	pobj	retina,of
R198	T445	T437	punct	.,suppresses
R1980	T3116	T3117	punct	(,3G
R1981	T3117	T3115	parataxis	3G,retina
R1982	T3118	T3117	compound	Figure,3G
R1983	T3119	T3120	punct	–,3O
R1984	T3120	T3117	prep	3O,3G
R1985	T3121	T3117	punct	),3G
R1986	T3122	T3103	punct	", ",compartments
R1987	T3123	T3103	cc	and,compartments
R1988	T3124	T3125	amod	dissociated,glia
R1989	T3125	T3103	conj	glia,compartments
R199	T447	T448	aux	To,probe
R1990	T3126	T3125	amod	primary,glia
R1991	T3127	T3125	cc	and,glia
R1992	T3128	T3129	compound	neuron,cultures
R1993	T3129	T3125	conj	cultures,glia
R1994	T3130	T3125	prep	from,glia
R1995	T3131	T3132	det	the,brain
R1996	T3132	T3130	pobj	brain,from
R1997	T3133	T3134	punct	(,3P
R1998	T3134	T3125	parataxis	3P,glia
R1999	T3135	T3134	compound	Figure,3P
R2	T306	T297	prep	in,role
R20	T250	T254	nsubj	protein,is
R200	T448	T449	advcl	probe,generated
R2000	T3136	T3137	punct	–,3Z
R2001	T3137	T3134	prep	3Z,3P
R2002	T3138	T3134	punct	),3P
R2003	T3139	T3057	punct	.,extended
R2004	T3141	T3142	amod	Double,immunostaining
R2005	T3142	T3143	nsubj	immunostaining,showed
R2006	T3144	T3142	prep	with,immunostaining
R2007	T3145	T3144	pobj	antibodies,with
R2008	T3146	T3145	prep	against,antibodies
R2009	T3147	T3148	det	these,proteins
R201	T450	T451	det	the,role
R2010	T3148	T3146	pobj	proteins,against
R2011	T3149	T3150	mark	that,colocalize
R2012	T3150	T3143	ccomp	colocalize,showed
R2013	T3151	T3150	nsubj	they,colocalize
R2014	T3152	T3150	prep	to,colocalize
R2015	T3153	T3154	det	the,mitochondria
R2016	T3154	T3152	pobj	mitochondria,to
R2017	T3155	T3156	punct	(,3A
R2018	T3156	T3150	parataxis	3A,colocalize
R2019	T3157	T3156	compound	Figure,3A
R202	T451	T448	dobj	role,probe
R2020	T3158	T3159	punct	–,3Z
R2021	T3159	T3156	prep	3Z,3A
R2022	T3160	T3156	punct	),3A
R2023	T3161	T3143	punct	.,showed
R2028	T3449	T3450	nsubj	Haploinsufficiency,Causes
R2029	T3451	T3449	prep	of,Haploinsufficiency
R203	T452	T451	amod	physiological,role
R2030	T3452	T3451	pobj	RanBP2,of
R2031	T3453	T3454	amod	Decreased,Levels
R2032	T3454	T3450	dobj	Levels,Causes
R2033	T3455	T3454	prep	of,Levels
R2034	T3456	T3455	pobj	HKI,of
R2035	T3457	T3454	cc	and,Levels
R2036	T3458	T3459	amod	Partial,Mislocalization
R2037	T3459	T3454	conj	Mislocalization,Levels
R2038	T3460	T3459	prep	of,Mislocalization
R2039	T3461	T3460	pobj	HKI,of
R204	T453	T451	prep	of,role
R2040	T3463	T3464	aux	To,determine
R2041	T3464	T3465	advcl	determine,employed
R2042	T3466	T3467	det	the,implications
R2043	T3467	T3464	dobj	implications,determine
R2044	T3468	T3467	amod	physiological,implications
R2045	T3469	T3467	prep	of,implications
R2046	T3470	T3471	det	the,interaction
R2047	T3471	T3469	pobj	interaction,of
R2048	T3472	T3471	prep	between,interaction
R2049	T3473	T3472	pobj	RanBP2,between
R205	T454	T453	pobj	RanBP2,of
R2050	T3474	T3473	cc	and,RanBP2
R2051	T3475	T3473	conj	HKI,RanBP2
R2052	T3476	T3475	punct	(,HKI
R2053	T3477	T3475	cc	and,HKI
R2054	T3478	T3479	amod	other,partners
R2055	T3479	T3475	conj	partners,HKI
R2056	T3480	T3465	punct	),employed
R2057	T3481	T3465	punct	", ",employed
R2058	T3482	T3465	nsubj	we,employed
R2059	T3483	T3484	det	a,line
R206	T455	T451	cc	and,role
R2060	T3484	T3465	dobj	line,employed
R2061	T3485	T3484	amod	murine,line
R2062	T3486	T3487	amod	embryonic,stem
R2063	T3487	T3484	compound	stem,line
R2064	T3488	T3484	compound	129Ola,line
R2065	T3489	T3484	compound	cell,line
R2066	T3490	T3484	prep	with,line
R2067	T3491	T3492	det	a,locus
R2068	T3492	T3490	pobj	locus,with
R2069	T3493	T3492	amod	targeted,locus
R207	T456	T457	poss	its,role
R2070	T3494	T3492	compound	RanBP2,locus
R2071	T3495	T3492	acl	produced,locus
R2072	T3496	T3495	agent	by,produced
R2073	T3497	T3498	compound	gene,trapping
R2074	T3498	T3496	pobj	trapping,by
R2075	T3499	T3498	punct	-,trapping
R2076	T3500	T3501	aux	to,produce
R2077	T3501	T3495	advcl	produce,produced
R2078	T3502	T3503	amod	stable,lines
R2079	T3503	T3501	dobj	lines,produce
R208	T457	T451	conj	role,role
R2080	T3504	T3503	amod	inbred,lines
R2081	T3505	T3506	punct	(,coisogenic
R2082	T3506	T3504	parataxis	coisogenic,inbred
R2083	T3507	T3506	punct	),coisogenic
R2084	T3508	T3504	cc	and,inbred
R2085	T3509	T3510	amod	mixed,background
R2086	T3510	T3504	conj	background,inbred
R2087	T3511	T3501	punct	", ",produce
R2088	T3512	T3501	advmod	respectively,produce
R2089	T3513	T3514	punct	(,4A
R209	T458	T457	prep	in,role
R2090	T3514	T3465	parataxis	4A,employed
R2091	T3515	T3514	compound	Figure,4A
R2092	T3516	T3514	cc	and,4A
R2093	T3517	T3514	conj	4B,4A
R2094	T3518	T3514	punct	),4A
R2095	T3519	T3465	punct	.,employed
R2096	T3521	T3522	nsubj	Genotyping,revealed
R2097	T3523	T3521	prep	of,Genotyping
R2098	T3524	T3525	nummod	299,F2
R2099	T3525	T3526	compound	F2,offspring
R21	T251	T252	npadvmod	Ran,binding
R210	T459	T460	compound	HKI,function
R2100	T3526	T3523	pobj	offspring,of
R2101	T3527	T3528	det	a,distribution
R2102	T3528	T3522	dobj	distribution,revealed
R2103	T3529	T3528	nmod	RanBP2,distribution
R2104	T3530	T3529	punct	+,RanBP2
R2105	T3531	T3529	punct	/,RanBP2
R2106	T3532	T3529	punct	+,RanBP2
R2107	T3533	T3529	punct	:,RanBP2
R2108	T3534	T3529	appos	RanBP2,RanBP2
R2109	T3535	T3534	punct	+,RanBP2
R211	T460	T458	pobj	function,in
R2110	T3536	T3534	punct	/,RanBP2
R2111	T3537	T3534	punct	−,RanBP2
R2112	T3538	T3529	punct	:,RanBP2
R2113	T3539	T3529	appos	RanBP2,RanBP2
R2114	T3540	T3539	punct	−,RanBP2
R2115	T3541	T3539	punct	/,RanBP2
R2116	T3542	T3539	punct	−,RanBP2
R2117	T3543	T3528	punct	(,distribution
R2118	T3544	T3545	nummod	89,0
R2119	T3545	T3528	appos	0,distribution
R212	T461	T449	punct	", ",generated
R2120	T3546	T3545	punct	:,0
R2121	T3547	T3545	nummod	210,0
R2122	T3548	T3545	punct	:,0
R2123	T3549	T3528	punct	),distribution
R2124	T3550	T3551	dep	that,deviated
R2125	T3551	T3528	relcl	deviated,distribution
R2126	T3552	T3551	prep	from,deviated
R2127	T3553	T3554	det	the,ratio
R2128	T3554	T3552	pobj	ratio,from
R2129	T3555	T3554	amod	expected,ratio
R213	T462	T463	det	a,model
R2130	T3556	T3554	amod	Mendelian,ratio
R2131	T3557	T3551	cc	and,deviated
R2132	T3558	T3551	conj	supports,deviated
R2133	T3559	T3560	mark	that,were
R2134	T3560	T3558	ccomp	were,supports
R2135	T3561	T3562	det	the,mice
R2136	T3562	T3560	nsubj	mice,were
R2137	T3563	T3562	nmod	RanBP2,mice
R2138	T3564	T3563	punct	−,RanBP2
R2139	T3565	T3563	punct	/,RanBP2
R214	T463	T449	nsubjpass	model,generated
R2140	T3566	T3563	punct	−,RanBP2
R2141	T3567	T3568	advmod	embryonically,lethal
R2142	T3568	T3560	acomp	lethal,were
R2143	T3569	T3522	punct	.,revealed
R2144	T3571	T3572	compound	E12.5,embryos
R2145	T3572	T3573	nsubj	embryos,show
R2146	T3574	T3575	amod	strong,expression
R2147	T3575	T3573	dobj	expression,show
R2148	T3576	T3575	prep	of,expression
R2149	T3577	T3576	pobj	RanBP2,of
R215	T464	T463	compound	mouse,model
R2150	T3578	T3575	prep	in,expression
R2151	T3579	T3580	det	the,vesicle
R2152	T3580	T3578	pobj	vesicle,in
R2153	T3581	T3580	amod	optic,vesicle
R2154	T3582	T3578	cc	and,in
R2155	T3583	T3578	conj	throughout,in
R2156	T3584	T3583	pobj	much,throughout
R2157	T3585	T3584	prep	of,much
R2158	T3586	T3587	det	the,embryo
R2159	T3587	T3585	pobj	embryo,of
R216	T465	T463	acl	harboring,model
R2160	T3588	T3587	punct	", ",embryo
R2161	T3589	T3590	dep	which,had
R2162	T3590	T3587	relcl	had,embryo
R2163	T3591	T3592	det	no,abnormalities
R2164	T3592	T3590	dobj	abnormalities,had
R2165	T3593	T3592	amod	apparent,abnormalities
R2166	T3594	T3592	amod	developmental,abnormalities
R2167	T3595	T3596	punct	(,4C
R2168	T3596	T3573	parataxis	4C,show
R2169	T3597	T3596	compound	Figure,4C
R217	T466	T467	det	a,locus
R2170	T3598	T3596	punct	),4C
R2171	T3599	T3573	punct	.,show
R2172	T3601	T3602	prep	In,expressed
R2173	T3603	T3601	pobj	agreement,In
R2174	T3604	T3603	prep	with,agreement
R2175	T3605	T3606	amod	previous,analysis
R2176	T3606	T3604	pobj	analysis,with
R2177	T3607	T3606	compound	immunocytochemistry,analysis
R2178	T3608	T3609	punct	[,19
R2179	T3609	T3606	parataxis	19,analysis
R218	T467	T465	dobj	locus,harboring
R2180	T3610	T3609	punct	],19
R2181	T3611	T3602	punct	", ",expressed
R2182	T3612	T3613	det	the,gene
R2183	T3613	T3602	nsubjpass	gene,expressed
R2184	T3614	T3613	compound	RanBP2,gene
R2185	T3615	T3602	auxpass	is,expressed
R2186	T3616	T3602	prep	across,expressed
R2187	T3617	T3618	amod	mature,neurons
R2188	T3618	T3616	pobj	neurons,across
R2189	T3619	T3618	amod	retinal,neurons
R219	T468	T469	advmod	genetically,disrupted
R2190	T3620	T3602	punct	", ",expressed
R2191	T3621	T3602	cc	but,expressed
R2192	T3622	T3623	nsubj	expression,was
R2193	T3623	T3602	conj	was,expressed
R2194	T3624	T3622	prep	in,expression
R2195	T3625	T3626	compound	ganglion,cells
R2196	T3626	T3624	pobj	cells,in
R2197	T3627	T3626	prep	of,cells
R2198	T3628	T3629	det	the,retina
R2199	T3629	T3627	pobj	retina,of
R22	T252	T250	amod	binding,protein
R220	T469	T467	amod	disrupted,locus
R2200	T3630	T3629	amod	adult,retina
R2201	T3631	T3632	advmod	extremely,strong
R2202	T3632	T3623	acomp	strong,was
R2203	T3633	T3634	punct	(,4D
R2204	T3634	T3623	parataxis	4D,was
R2205	T3635	T3634	compound	Figure,4D
R2206	T3636	T3634	cc	and,4D
R2207	T3637	T3634	conj	4E,4D
R2208	T3638	T3634	punct	),4D
R2209	T3639	T3623	punct	.,was
R221	T470	T467	compound	RanBP2,locus
R2210	T3641	T3642	prep	In,probed
R2211	T3643	T3641	pobj	light,In
R2212	T3644	T3643	prep	of,light
R2213	T3645	T3646	det	the,association
R2214	T3646	T3644	pobj	association,of
R2215	T3647	T3648	advmod	in,vivo
R2216	T3648	T3646	advmod	vivo,association
R2217	T3649	T3646	prep	of,association
R2218	T3650	T3649	pobj	RanBP2,of
R2219	T3651	T3646	prep	with,association
R222	T471	T449	auxpass	was,generated
R2220	T3652	T3651	pobj	Cox11,with
R2221	T3653	T3652	cc	and,Cox11
R2222	T3654	T3652	conj	HKI,Cox11
R2223	T3655	T3656	punct	(,1
R2224	T3656	T3646	parataxis	1,association
R2225	T3657	T3656	nmod	Figures,1
R2226	T3658	T3656	cc	and,1
R2227	T3659	T3656	conj	3,1
R2228	T3660	T3656	punct	),1
R2229	T3661	T3646	punct	", ",association
R223	T472	T449	punct	.,generated
R2230	T3662	T3663	amod	profound,modulation
R2231	T3663	T3646	conj	modulation,association
R2232	T3664	T3665	advmod	in,vitro
R2233	T3665	T3663	amod	vitro,modulation
R2234	T3666	T3663	prep	of,modulation
R2235	T3667	T3668	npadvmod	HKI,enzymatic
R2236	T3668	T3669	amod	enzymatic,activity
R2237	T3669	T3666	pobj	activity,of
R2238	T3670	T3663	prep	by,modulation
R2239	T3671	T3670	pobj	RanBP2,by
R224	T474	T475	nsubj	RanBP2,are
R2240	T3672	T3671	cc	and,RanBP2
R2241	T3673	T3671	conj	Cox11,RanBP2
R2242	T3674	T3675	punct	(,Figure
R2243	T3675	T3663	parataxis	Figure,modulation
R2244	T3676	T3675	nummod	2,Figure
R2245	T3677	T3675	punct	),Figure
R2246	T3678	T3663	punct	", ",modulation
R2247	T3679	T3663	cc	and,modulation
R2248	T3680	T3681	det	the,role
R2249	T3681	T3663	conj	role,modulation
R225	T476	T474	punct	−,RanBP2
R2250	T3682	T3681	amod	critical,role
R2251	T3683	T3681	prep	of,role
R2252	T3684	T3683	pobj	HKI,of
R2253	T3685	T3681	prep	in,role
R2254	T3686	T3685	pcomp	catalyzing,in
R2255	T3687	T3688	det	a,step
R2256	T3688	T3686	dobj	step,catalyzing
R2257	T3689	T3690	npadvmod	rate,limiting
R2258	T3690	T3688	amod	limiting,step
R2259	T3691	T3690	punct	-,limiting
R226	T477	T474	punct	/,RanBP2
R2260	T3692	T3688	prep	of,step
R2261	T3693	T3692	pobj	glycolysis,of
R2262	T3694	T3642	punct	", ",probed
R2263	T3695	T3642	nsubj	we,probed
R2264	T3696	T3697	mark	whether,presented
R2265	T3697	T3642	ccomp	presented,probed
R2266	T3698	T3699	nmod	RanBP2,mice
R2267	T3699	T3697	nsubj	mice,presented
R2268	T3700	T3698	punct	+,RanBP2
R2269	T3701	T3698	punct	/,RanBP2
R227	T478	T474	punct	−,RanBP2
R2270	T3702	T3698	punct	−,RanBP2
R2271	T3703	T3697	dobj	disturbances,presented
R2272	T3704	T3703	prep	in,disturbances
R2273	T3705	T3704	pobj	HKI,in
R2274	T3706	T3705	punct	", ",HKI
R2275	T3707	T3705	conj	Cox11,HKI
R2276	T3708	T3707	punct	", ",Cox11
R2277	T3709	T3707	cc	and,Cox11
R2278	T3710	T3711	compound	energy,homeostasis
R2279	T3711	T3707	conj	homeostasis,Cox11
R228	T479	T480	advmod	embryonically,lethal
R2280	T3712	T3642	punct	.,probed
R2281	T3714	T3715	amod	Monoallelic,expression
R2282	T3715	T3716	nsubj	expression,affect
R2283	T3717	T3715	prep	of,expression
R2284	T3718	T3717	pobj	RanBP2,of
R2285	T3719	T3716	aux	does,affect
R2286	T3720	T3716	neg	not,affect
R2287	T3721	T3722	det	the,number
R2288	T3722	T3716	dobj	number,affect
R2289	T3723	T3722	prep	of,number
R229	T480	T475	acomp	lethal,are
R2290	T3724	T3723	pobj	NPCs,of
R2291	T3725	T3722	cc	and,number
R2292	T3726	T3727	poss	their,distribution
R2293	T3727	T3722	conj	distribution,number
R2294	T3728	T3727	prep	in,distribution
R2295	T3729	T3730	amod	hippocampal,neurons
R2296	T3730	T3728	pobj	neurons,in
R2297	T3731	T3732	punct	(,data
R2298	T3732	T3716	parataxis	data,affect
R2299	T3733	T3734	compound	Figure,5A
R23	T253	T252	punct	-,binding
R230	T481	T475	punct	", ",are
R2300	T3734	T3732	dep	5A,data
R2301	T3735	T3732	punct	", ",data
R2302	T3736	T3732	amod	unpublished,data
R2303	T3737	T3732	punct	),data
R2304	T3738	T3716	punct	", ",affect
R2305	T3739	T3716	cc	but,affect
R2306	T3740	T3741	nsubj	it,led
R2307	T3741	T3716	conj	led,affect
R2308	T3742	T3741	prep	to,led
R2309	T3743	T3744	amod	consistent,expression
R231	T482	T475	cc	and,are
R2310	T3744	T3742	pobj	expression,to
R2311	T3745	T3744	amod	lower,expression
R2312	T3746	T3744	prep	of,expression
R2313	T3747	T3746	pobj	RanBP2,of
R2314	T3748	T3744	prep	by,expression
R2315	T3749	T3750	amod	more,50
R2316	T3750	T3752	nummod	50,%
R2317	T3751	T3750	quantmod	than,50
R2318	T3752	T3748	pobj	%,by
R2319	T3753	T3744	prep	in,expression
R232	T483	T484	nsubj	haploinsufficiency,causes
R2320	T3754	T3755	det	the,CNS
R2321	T3755	T3753	pobj	CNS,in
R2322	T3756	T3755	punct	(,CNS
R2323	T3757	T3755	appos	brain,CNS
R2324	T3758	T3757	cc	and,brain
R2325	T3759	T3757	conj	retina,brain
R2326	T3760	T3755	punct	),CNS
R2327	T3761	T3755	prep	in,CNS
R2328	T3762	T3761	pobj	129Ola,in
R2329	T3763	T3764	punct	(,5B
R233	T484	T475	conj	causes,are
R2330	T3764	T3762	parataxis	5B,129Ola
R2331	T3765	T3764	compound	Figure,5B
R2332	T3766	T3767	punct	–,5D
R2333	T3767	T3764	prep	5D,5B
R2334	T3768	T3764	punct	),5B
R2335	T3769	T3761	punct	", ",in
R2336	T3770	T3761	cc	but,in
R2337	T3771	T3770	neg	not,but
R2338	T3772	T3761	conj	in,in
R2339	T3773	T3774	compound	C57BL,129Ola
R234	T485	T483	prep	of,haploinsufficiency
R2340	T3774	T3778	compound	129Ola,backgrounds
R2341	T3775	T3774	punct	/,129Ola
R2342	T3776	T3774	compound	6J,129Ola
R2343	T3777	T3774	punct	/,129Ola
R2344	T3778	T3772	pobj	backgrounds,in
R2345	T3779	T3780	punct	(,data
R2346	T3780	T3741	meta	data,led
R2347	T3781	T3780	amod	unpublished,data
R2348	T3782	T3780	punct	),data
R2349	T3783	T3741	punct	.,led
R235	T486	T485	pobj	RanBP2,of
R2350	T3785	T3786	advmod	Hence,focused
R2351	T3787	T3786	punct	", ",focused
R2352	T3788	T3786	nsubj	we,focused
R2353	T3789	T3790	poss	our,analysis
R2354	T3790	T3786	dobj	analysis,focused
R2355	T3791	T3786	prep	on,focused
R2356	T3792	T3793	det	the,line
R2357	T3793	T3791	pobj	line,on
R2358	T3794	T3793	amod	inbred,line
R2359	T3795	T3793	nmod	RanBP2,line
R236	T487	T483	prep	in,haploinsufficiency
R2360	T3796	T3795	punct	+,RanBP2
R2361	T3797	T3795	punct	/,RanBP2
R2362	T3798	T3795	punct	−,RanBP2
R2363	T3799	T3793	compound	129Ola,line
R2364	T3800	T3793	compound	mouse,line
R2365	T3801	T3786	punct	.,focused
R2366	T3803	T3804	mark	Although,decreased
R2367	T3804	T3810	advcl	decreased,remained
R2368	T3805	T3806	det	the,levels
R2369	T3806	T3804	nsubjpass	levels,decreased
R237	T488	T489	det	an,strain
R2370	T3807	T3806	prep	of,levels
R2371	T3808	T3807	pobj	RanBP2,of
R2372	T3809	T3804	auxpass	were,decreased
R2373	T3811	T3804	prep	in,decreased
R2374	T3812	T3813	det	the,retina
R2375	T3813	T3811	pobj	retina,in
R2376	T3814	T3813	punct	", ",retina
R2377	T3815	T3813	conj	brain,retina
R2378	T3816	T3815	punct	", ",brain
R2379	T3817	T3815	cc	and,brain
R238	T489	T487	pobj	strain,in
R2380	T3818	T3815	conj	hippocampus,brain
R2381	T3819	T3813	prep	of,retina
R2382	T3820	T3821	nmod	RanBP2,mice
R2383	T3821	T3819	pobj	mice,of
R2384	T3822	T3820	punct	+,RanBP2
R2385	T3823	T3820	punct	/,RanBP2
R2386	T3824	T3820	punct	−,RanBP2
R2387	T3825	T3804	prep	by,decreased
R2388	T3826	T3827	punct	~,50
R2389	T3827	T3828	nummod	50,%
R239	T490	T489	amod	inbred,strain
R2390	T3828	T3825	pobj	%,by
R2391	T3829	T3830	punct	–,%
R2392	T3830	T3828	prep	%,%
R2393	T3831	T3830	nummod	60,%
R2394	T3832	T3833	punct	(,5B
R2395	T3833	T3804	parataxis	5B,decreased
R2396	T3834	T3833	compound	Figure,5B
R2397	T3835	T3833	cc	and,5B
R2398	T3836	T3833	conj	5C,5B
R2399	T3837	T3833	punct	),5B
R24	T255	T250	nummod	2,protein
R240	T491	T492	det	a,decrease
R2400	T3838	T3810	punct	", ",remained
R2401	T3839	T3840	det	the,levels
R2402	T3840	T3810	nsubj	levels,remained
R2403	T3841	T3840	prep	of,levels
R2404	T3842	T3843	compound	others,nucleoporins
R2405	T3843	T3841	pobj	nucleoporins,of
R2406	T3844	T3843	punct	", ",nucleoporins
R2407	T3845	T3843	appos	Nup153,nucleoporins
R2408	T3846	T3845	cc	and,Nup153
R2409	T3847	T3845	conj	Nup62,Nup153
R241	T492	T484	dobj	decrease,causes
R2410	T3848	T3843	punct	", ",nucleoporins
R2411	T3849	T3843	cc	and,nucleoporins
R2412	T3850	T3843	conj	mHsp70,nucleoporins
R2413	T3851	T3850	cc	and,mHsp70
R2414	T3852	T3850	conj	Cox11,mHsp70
R2415	T3853	T3810	punct	", ",remained
R2416	T3854	T3810	acomp	unchanged,remained
R2417	T3855	T3856	punct	(,data
R2418	T3856	T3810	parataxis	data,remained
R2419	T3857	T3858	compound	Figure,5B
R242	T493	T492	amod	pronounced,decrease
R2420	T3858	T3856	dep	5B,data
R2421	T3859	T3856	punct	", ",data
R2422	T3860	T3856	amod	unpublished,data
R2423	T3861	T3856	punct	),data
R2424	T3862	T3810	punct	.,remained
R2425	T3864	T3865	prep	In,observed
R2426	T3866	T3864	pobj	addition,In
R2427	T3867	T3865	punct	", ",observed
R2428	T3868	T3865	nsubj	we,observed
R2429	T3869	T3870	det	a,decrease
R243	T494	T492	prep	of,decrease
R2430	T3870	T3865	dobj	decrease,observed
R2431	T3871	T3870	amod	strong,decrease
R2432	T3872	T3870	prep	of,decrease
R2433	T3873	T3874	det	the,levels
R2434	T3874	T3872	pobj	levels,of
R2435	T3875	T3874	prep	of,levels
R2436	T3876	T3875	pobj	HKI,of
R2437	T3877	T3870	punct	(,decrease
R2438	T3878	T3870	appos	3,decrease
R2439	T3879	T3878	punct	-,3
R244	T495	T496	nmod	HKI,levels
R2440	T3880	T3878	quantmod	to,3
R2441	T3881	T3878	advmod	4-fold,3
R2442	T3882	T3865	punct	),observed
R2443	T3883	T3884	punct	(,5B
R2444	T3884	T3865	parataxis	5B,observed
R2445	T3885	T3884	compound	Figure,5B
R2446	T3886	T3884	cc	and,5B
R2447	T3887	T3884	conj	5C,5B
R2448	T3888	T3884	punct	),5B
R2449	T3889	T3865	punct	.,observed
R245	T496	T494	pobj	levels,of
R2450	T3891	T3892	det	This,decrease
R2451	T3892	T3893	nsubj	decrease,was
R2452	T3894	T3893	acomp	selective,was
R2453	T3895	T3894	prep	to,selective
R2454	T3896	T3897	det	the,CNS
R2455	T3897	T3895	pobj	CNS,to
R2456	T3898	T3893	punct	", ",was
R2457	T3899	T3900	mark	since,remained
R2458	T3900	T3893	advcl	remained,was
R2459	T3901	T3902	compound	HKI,levels
R246	T497	T495	cc	and,HKI
R2460	T3902	T3900	nsubj	levels,remained
R2461	T3903	T3904	advmod	largely,unaffected
R2462	T3904	T3900	acomp	unaffected,remained
R2463	T3905	T3900	prep	in,remained
R2464	T3906	T3907	det	the,muscle
R2465	T3907	T3905	pobj	muscle,in
R2466	T3908	T3907	amod	skeletal,muscle
R2467	T3909	T3907	punct	", ",muscle
R2468	T3910	T3907	conj	spleen,muscle
R2469	T3911	T3910	punct	", ",spleen
R247	T498	T495	conj	ATP,HKI
R2470	T3912	T3910	cc	and,spleen
R2471	T3913	T3910	conj	liver,spleen
R2472	T3914	T3915	punct	(,5D
R2473	T3915	T3893	parataxis	5D,was
R2474	T3916	T3915	compound	Figure,5D
R2475	T3917	T3915	punct	),5D
R2476	T3918	T3893	punct	.,was
R2477	T3920	T3921	mark	Because,plays
R2478	T3921	T3923	advcl	plays,probed
R2479	T3922	T3921	nsubj	HKI,plays
R248	T499	T492	advmod	selectively,decrease
R2480	T3924	T3925	det	a,role
R2481	T3925	T3921	dobj	role,plays
R2482	T3926	T3925	amod	key,role
R2483	T3927	T3921	prep	in,plays
R2484	T3928	T3929	det	the,production
R2485	T3929	T3927	pobj	production,in
R2486	T3930	T3929	prep	of,production
R2487	T3931	T3932	nmod	energy,substrates
R2488	T3932	T3930	pobj	substrates,of
R2489	T3933	T3932	amod	intermediate,substrates
R249	T500	T492	prep	in,decrease
R2490	T3934	T3921	cc	and,plays
R2491	T3935	T3936	nsubj	HKI,is
R2492	T3936	T3921	conj	is,plays
R2493	T3937	T3938	advmod	virtually,isoform
R2494	T3938	T3936	attr	isoform,is
R2495	T3939	T3938	det	the,isoform
R2496	T3940	T3938	amod	sole,isoform
R2497	T3941	T3938	compound	HK,isoform
R2498	T3942	T3938	acl	expressed,isoform
R2499	T3943	T3942	prep	in,expressed
R25	T256	T250	punct	(,protein
R250	T501	T502	det	the,system
R2500	T3944	T3945	det	the,CNS
R2501	T3945	T3943	pobj	CNS,in
R2502	T3946	T3947	punct	[,34
R2503	T3947	T3936	parataxis	34,is
R2504	T3948	T3947	nummod	33,34
R2505	T3949	T3947	punct	",",34
R2506	T3950	T3947	punct	],34
R2507	T3951	T3923	punct	", ",probed
R2508	T3952	T3923	nsubj	we,probed
R2509	T3953	T3954	det	the,impact
R251	T502	T500	pobj	system,in
R2510	T3954	T3923	dobj	impact,probed
R2511	T3955	T3954	prep	of,impact
R2512	T3956	T3957	nmod	HKI,reduction
R2513	T3957	T3955	pobj	reduction,of
R2514	T3958	T3956	cc	and,HKI
R2515	T3959	T3956	conj	RanBP2,HKI
R2516	T3960	T3954	prep	in,impact
R2517	T3961	T3962	det	the,levels
R2518	T3962	T3960	pobj	levels,in
R2519	T3963	T3962	prep	of,levels
R252	T503	T502	amod	central,system
R2520	T3964	T3963	pobj	ATP,of
R2521	T3965	T3923	punct	.,probed
R2522	T3967	T3968	mark	As,shown
R2523	T3968	T3969	advcl	shown,was
R2524	T3970	T3968	prep	in,shown
R2525	T3971	T3972	compound	Figure,5E
R2526	T3972	T3970	pobj	5E,in
R2527	T3973	T3969	punct	", ",was
R2528	T3974	T3969	expl	there,was
R2529	T3975	T3976	amod	significant,reduction
R253	T504	T502	amod	nervous,system
R2530	T3976	T3969	attr	reduction,was
R2531	T3977	T3975	cc	and,significant
R2532	T3978	T3975	conj	concordant,significant
R2533	T3979	T3976	prep	in,reduction
R2534	T3980	T3979	pobj	levels,in
R2535	T3981	T3980	prep	of,levels
R2536	T3982	T3981	pobj	ATP,of
R2537	T3983	T3980	prep	in,levels
R2538	T3984	T3985	det	the,CNS
R2539	T3985	T3983	pobj	CNS,in
R254	T505	T484	punct	.,causes
R2540	T3986	T3985	punct	(,CNS
R2541	T3987	T3985	appos	brain,CNS
R2542	T3988	T3987	cc	and,brain
R2543	T3989	T3987	conj	retina,brain
R2544	T3990	T3983	punct	),in
R2545	T3991	T3983	punct	", ",in
R2546	T3992	T3983	cc	but,in
R2547	T3993	T3992	neg	not,but
R2548	T3994	T3983	conj	in,in
R2549	T3995	T3996	amod	non-neuronal,tissues
R255	T507	T508	amod	Inbred,mice
R2550	T3996	T3994	pobj	tissues,in
R2551	T3997	T3969	punct	.,was
R2552	T3999	T4000	advmod	Finally,observed
R2553	T4001	T4000	punct	", ",observed
R2554	T4002	T4000	nsubj	we,observed
R2555	T4003	T4000	advmod	also,observed
R2556	T4004	T4005	amod	partial,delocalization
R2557	T4005	T4000	dobj	delocalization,observed
R2558	T4006	T4004	cc	and,partial
R2559	T4007	T4004	conj	selective,partial
R256	T508	T513	nsubj	mice,exhibit
R2560	T4008	T4005	prep	of,delocalization
R2561	T4009	T4008	pobj	HKI,of
R2562	T4010	T4008	punct	", ",of
R2563	T4011	T4008	cc	but,of
R2564	T4012	T4011	neg	not,but
R2565	T4013	T4008	conj	of,of
R2566	T4014	T4013	pobj	Cox11,of
R2567	T4015	T4005	punct	", ",delocalization
R2568	T4016	T4005	prep	from,delocalization
R2569	T4017	T4018	det	the,ellipsoid
R257	T509	T508	nmod	RanBP2,mice
R2570	T4018	T4016	pobj	ellipsoid,from
R2571	T4019	T4018	punct	(,ellipsoid
R2572	T4020	T4021	npadvmod	mitochondria,rich
R2573	T4021	T4018	amod	rich,ellipsoid
R2574	T4022	T4021	punct	-,rich
R2575	T4023	T4018	punct	),ellipsoid
R2576	T4024	T4016	prep	to,from
R2577	T4025	T4026	det	the,myoid
R2578	T4026	T4024	pobj	myoid,to
R2579	T4027	T4026	amod	adjacent,myoid
R258	T510	T509	punct	+,RanBP2
R2580	T4028	T4029	amod	subcellular,compartment
R2581	T4029	T4016	pobj	compartment,from
R2582	T4030	T4029	prep	of,compartment
R2583	T4031	T4032	nmod	rod,neurons
R2584	T4032	T4030	pobj	neurons,of
R2585	T4033	T4032	amod	photosensory,neurons
R2586	T4034	T4035	punct	(,S2A
R2587	T4035	T4005	parataxis	S2A,delocalization
R2588	T4036	T4035	compound	Figure,S2A
R2589	T4037	T4038	punct	–,S2E
R259	T511	T509	punct	/,RanBP2
R2590	T4038	T4035	prep	S2E,S2A
R2591	T4039	T4035	punct	),S2A
R2592	T4040	T4000	punct	.,observed
R2593	T4042	T4043	nsubjpass	This,accompanied
R2594	T4044	T4043	auxpass	was,accompanied
R2595	T4045	T4043	advmod	also,accompanied
R2596	T4046	T4043	agent	by,accompanied
R2597	T4047	T4048	amod	reduced,levels
R2598	T4048	T4046	pobj	levels,by
R2599	T4049	T4048	compound	HKI,levels
R26	T257	T250	appos	RanBP2,protein
R260	T512	T509	punct	−,RanBP2
R2600	T4050	T4048	prep	in,levels
R2601	T4051	T4052	det	the,retina
R2602	T4052	T4050	pobj	retina,in
R2603	T4053	T4052	amod	inner,retina
R2604	T4054	T4050	punct	", ",in
R2605	T4055	T4056	prep	in,in
R2606	T4056	T4050	prep	in,in
R2607	T4057	T4055	amod	particular,in
R2608	T4058	T4059	det	the,layer
R2609	T4059	T4056	pobj	layer,in
R261	T514	T513	advmod	also,exhibit
R2610	T4060	T4059	amod	inner,layer
R2611	T4061	T4059	amod	plexiform,layer
R2612	T4062	T4059	punct	(,layer
R2613	T4063	T4059	amod	synaptic,layer
R2614	T4064	T4059	punct	),layer
R2615	T4065	T4066	punct	(,S2A
R2616	T4066	T4043	parataxis	S2A,accompanied
R2617	T4067	T4066	compound	Figure,S2A
R2618	T4068	T4069	punct	–,S2E
R2619	T4069	T4066	prep	S2E,S2A
R262	T515	T513	dobj	deficits,exhibit
R2620	T4070	T4066	punct	),S2A
R2621	T4071	T4043	punct	.,accompanied
R2626	T4308	T4309	amod	Metabolic,Disturbances
R2627	T4310	T4309	acl	Caused,Disturbances
R2628	T4311	T4310	agent	by,Caused
R2629	T4312	T4311	pobj	Haploinsufficiency,by
R263	T516	T515	prep	in,deficits
R2630	T4313	T4312	prep	of,Haploinsufficiency
R2631	T4314	T4313	pobj	RanBP2,of
R2632	T4316	T4317	det	The,rates
R2633	T4317	T4319	nsubj	rates,were
R2634	T4318	T4317	compound	growth,rates
R2635	T4320	T4317	prep	of,rates
R2636	T4321	T4322	amod	inbred,mice
R2637	T4322	T4320	pobj	mice,of
R2638	T4323	T4322	nmod	RanBP2,mice
R2639	T4324	T4323	punct	+,RanBP2
R264	T517	T518	compound	growth,rates
R2640	T4325	T4323	punct	/,RanBP2
R2641	T4326	T4323	punct	−,RanBP2
R2642	T4327	T4322	prep	on,mice
R2643	T4328	T4329	amod	high,fat
R2644	T4329	T4331	nmod	fat,diet
R2645	T4330	T4329	punct	-,fat
R2646	T4331	T4327	pobj	diet,on
R2647	T4332	T4329	punct	(,fat
R2648	T4333	T4334	punct	~,10
R2649	T4334	T4335	nummod	10,%
R265	T518	T516	pobj	rates,in
R2650	T4335	T4336	compound	%,fat
R2651	T4336	T4329	appos	fat,fat
R2652	T4337	T4331	punct	),diet
R2653	T4338	T4339	advmod	significantly,slower
R2654	T4339	T4319	acomp	slower,were
R2655	T4340	T4339	prep	than,slower
R2656	T4341	T4342	nmod	RanBP2,mice
R2657	T4342	T4340	pobj	mice,than
R2658	T4343	T4341	punct	+,RanBP2
R2659	T4344	T4341	punct	/,RanBP2
R266	T519	T518	cc	and,rates
R2660	T4345	T4341	punct	+,RanBP2
R2661	T4346	T4347	punct	(,6A
R2662	T4347	T4319	parataxis	6A,were
R2663	T4348	T4347	compound	Figure,6A
R2664	T4349	T4347	punct	),6A
R2665	T4350	T4319	punct	.,were
R2666	T4352	T4353	advcl	Beginning,exhibit
R2667	T4354	T4352	prep	at,Beginning
R2668	T4355	T4356	quantmod	around,4
R2669	T4356	T4357	nummod	4,mo
R267	T520	T521	compound	glucose,catabolism
R2670	T4357	T4354	pobj	mo,at
R2671	T4358	T4357	prep	of,mo
R2672	T4359	T4358	pobj	age,of
R2673	T4360	T4353	punct	", ",exhibit
R2674	T4361	T4362	nmod	RanBP2,mice
R2675	T4362	T4353	nsubj	mice,exhibit
R2676	T4363	T4361	punct	+,RanBP2
R2677	T4364	T4361	punct	/,RanBP2
R2678	T4365	T4361	punct	−,RanBP2
R2679	T4366	T4367	det	a,gain
R268	T521	T518	conj	catabolism,rates
R2680	T4367	T4353	dobj	gain,exhibit
R2681	T4368	T4369	amod	significant,slower
R2682	T4369	T4367	amod	slower,gain
R2683	T4370	T4367	prep	in,gain
R2684	T4371	T4372	compound	body,mass
R2685	T4372	T4370	pobj	mass,in
R2686	T4373	T4367	prep	than,gain
R2687	T4374	T4375	amod	wild,type
R2688	T4375	T4377	compound	type,mice
R2689	T4376	T4375	punct	-,type
R269	T522	T513	prep	without,exhibit
R2690	T4377	T4373	pobj	mice,than
R2691	T4378	T4379	punct	(,6A
R2692	T4379	T4353	parataxis	6A,exhibit
R2693	T4380	T4379	compound	Figure,6A
R2694	T4381	T4379	punct	),6A
R2695	T4382	T4353	punct	.,exhibit
R2696	T4384	T4385	prep	In,presented
R2697	T4386	T4384	pobj	addition,In
R2698	T4387	T4385	punct	", ",presented
R2699	T4388	T4389	nmod	RanBP2,mice
R27	T258	T254	punct	),is
R270	T523	T522	pobj	impairment,without
R2700	T4389	T4385	nsubj	mice,presented
R2701	T4390	T4388	punct	+,RanBP2
R2702	T4391	T4388	punct	/,RanBP2
R2703	T4392	T4388	punct	−,RanBP2
R2704	T4393	T4389	amod	inbred,mice
R2705	T4394	T4385	dobj	deficits,presented
R2706	T4395	T4394	prep	in,deficits
R2707	T4396	T4397	compound	body,mass
R2708	T4397	T4395	pobj	mass,in
R2709	T4398	T4399	dep	that,erased
R271	T524	T523	prep	of,impairment
R2710	T4399	T4394	relcl	erased,deficits
R2711	T4400	T4399	auxpass	were,erased
R2712	T4401	T4399	prep	by,erased
R2713	T4402	T4401	pcomp	changing,by
R2714	T4403	T4404	det	the,background
R2715	T4404	T4402	dobj	background,changing
R2716	T4405	T4404	amod	genetic,background
R2717	T4406	T4402	prep	to,changing
R2718	T4407	T4408	det	a,129Ola
R2719	T4408	T4406	pobj	129Ola,to
R272	T525	T526	compound	glucose,uptake
R2720	T4409	T4408	amod	mixed,129Ola
R2721	T4410	T4408	compound	C57BL,129Ola
R2722	T4411	T4408	punct	/,129Ola
R2723	T4412	T4408	compound	6J,129Ola
R2724	T4413	T4408	punct	/,129Ola
R2725	T4414	T4415	punct	(,6B
R2726	T4415	T4385	parataxis	6B,presented
R2727	T4416	T4415	compound	Figure,6B
R2728	T4417	T4415	punct	),6B
R2729	T4418	T4385	punct	.,presented
R273	T526	T524	pobj	uptake,of
R2730	T4420	T4421	compound	Food,consumption
R2731	T4421	T4422	nsubj	consumption,account
R2732	T4423	T4422	aux	did,account
R2733	T4424	T4422	neg	not,account
R2734	T4425	T4422	prep	for,account
R2735	T4426	T4427	det	the,differences
R2736	T4427	T4425	pobj	differences,for
R2737	T4428	T4429	compound	body,weight
R2738	T4429	T4427	compound	weight,differences
R2739	T4430	T4427	acl	observed,differences
R274	T527	T526	cc	and,uptake
R2740	T4431	T4432	punct	(,6C
R2741	T4432	T4422	parataxis	6C,account
R2742	T4433	T4432	compound	Figure,6C
R2743	T4434	T4432	punct	),6C
R2744	T4435	T4422	punct	.,account
R2745	T4437	T4438	nsubj	HKI,accounts
R2746	T4439	T4437	prep	in,HKI
R2747	T4440	T4441	det	the,CNS
R2748	T4441	T4439	pobj	CNS,in
R2749	T4442	T4441	punct	(,CNS
R275	T528	T526	conj	gluconeogenesis,uptake
R2750	T4443	T4441	appos	brain,CNS
R2751	T4444	T4443	cc	and,brain
R2752	T4445	T4443	conj	retina,brain
R2753	T4446	T4438	punct	),accounts
R2754	T4447	T4438	advmod	virtually,accounts
R2755	T4448	T4438	prep	for,accounts
R2756	T4449	T4450	det	all,expression
R2757	T4450	T4448	pobj	expression,for
R2758	T4451	T4450	prep	of,expression
R2759	T4452	T4453	compound	HK,isozymes
R276	T529	T513	punct	.,exhibit
R2760	T4453	T4451	pobj	isozymes,of
R2761	T4454	T4450	cc	and,expression
R2762	T4455	T4456	compound	glucose,utilization
R2763	T4456	T4450	conj	utilization,expression
R2764	T4457	T4450	prep	in,expression
R2765	T4458	T4459	det	the,CNS
R2766	T4459	T4457	pobj	CNS,in
R2767	T4460	T4461	punct	[,34
R2768	T4461	T4438	parataxis	34,accounts
R2769	T4462	T4461	nummod	33,34
R277	T531	T532	det	These,phenotypes
R2770	T4463	T4461	punct	",",34
R2771	T4464	T4461	punct	],34
R2772	T4465	T4438	punct	.,accounts
R2773	T4467	T4468	advmod	Moreover,is
R2774	T4469	T4468	punct	", ",is
R2775	T4470	T4468	nsubj	glucose,is
R2776	T4471	T4472	det	the,source
R2777	T4472	T4468	attr	source,is
R2778	T4473	T4472	amod	sole,source
R2779	T4474	T4472	compound	reliance,source
R278	T532	T533	nsubjpass	phenotypes,accompanied
R2780	T4475	T4472	prep	of,source
R2781	T4476	T4475	pobj	energy,of
R2782	T4477	T4472	prep	in,source
R2783	T4478	T4479	det	the,CNS
R2784	T4479	T4477	pobj	CNS,in
R2785	T4480	T4472	prep	under,source
R2786	T4481	T4482	amod	normal,conditions
R2787	T4482	T4480	pobj	conditions,under
R2788	T4483	T4468	punct	", ",is
R2789	T4484	T4485	det	the,CNS
R279	T534	T533	auxpass	are,accompanied
R2790	T4485	T4486	nsubj	CNS,lacks
R2791	T4486	T4468	conj	lacks,is
R2792	T4487	T4488	compound	glucose,storage
R2793	T4488	T4489	compound	storage,sources
R2794	T4489	T4486	dobj	sources,lacks
R2795	T4490	T4486	punct	", ",lacks
R2796	T4491	T4486	cc	and,lacks
R2797	T4492	T4493	prep	despite,consumes
R2798	T4493	T4486	conj	consumes,lacks
R2799	T4494	T4495	det	the,mass
R28	T259	T260	det	a,protein
R280	T535	T533	agent	by,accompanied
R2800	T4495	T4492	pobj	mass,despite
R2801	T4496	T4495	amod	disproportionate,mass
R2802	T4497	T4495	prep	of,mass
R2803	T4498	T4499	det	the,CNS
R2804	T4499	T4497	pobj	CNS,of
R2805	T4500	T4495	prep	to,mass
R2806	T4501	T4502	det	the,rest
R2807	T4502	T4500	pobj	rest,to
R2808	T4503	T4502	prep	of,rest
R2809	T4504	T4505	det	the,body
R281	T536	T537	det	a,decrease
R2810	T4505	T4503	pobj	body,of
R2811	T4506	T4493	punct	", ",consumes
R2812	T4507	T4508	det	the,CNS
R2813	T4508	T4493	nsubj	CNS,consumes
R2814	T4509	T4493	advmod	daily,consumes
R2815	T4510	T4511	quantmod	about,60
R2816	T4511	T4512	nummod	60,%
R2817	T4512	T4493	dobj	%,consumes
R2818	T4513	T4512	prep	of,%
R2819	T4514	T4515	det	the,body
R282	T537	T535	pobj	decrease,by
R2820	T4515	T4516	poss	body,glucose
R2821	T4516	T4513	pobj	glucose,of
R2822	T4517	T4515	case	's,body
R2823	T4518	T4512	cc	and,%
R2824	T4519	T4520	nummod	25,%
R2825	T4520	T4512	conj	%,%
R2826	T4521	T4520	prep	of,%
R2827	T4522	T4523	det	the,oxygen
R2828	T4523	T4521	pobj	oxygen,of
R2829	T4524	T4523	amod	total,oxygen
R283	T538	T537	prep	in,decrease
R2830	T4525	T4526	punct	[,36
R2831	T4526	T4493	parataxis	36,consumes
R2832	T4527	T4526	nummod	35,36
R2833	T4528	T4526	punct	",",36
R2834	T4529	T4526	punct	],36
R2835	T4530	T4493	punct	.,consumes
R2836	T4532	T4533	aux	To,determine
R2837	T4533	T4534	advcl	determine,carried
R2838	T4535	T4536	det	the,impact
R2839	T4536	T4533	dobj	impact,determine
R284	T539	T540	det	the,responses
R2840	T4537	T4536	prep	of,impact
R2841	T4538	T4539	compound	RanBP2,haploinsufficiency
R2842	T4539	T4537	pobj	haploinsufficiency,of
R2843	T4540	T4536	prep	on,impact
R2844	T4541	T4542	det	the,utilization
R2845	T4542	T4540	pobj	utilization,on
R2846	T4543	T4542	punct	", ",utilization
R2847	T4544	T4542	conj	formation,utilization
R2848	T4545	T4544	punct	", ",formation
R2849	T4546	T4544	cc	and,formation
R285	T540	T538	pobj	responses,in
R2850	T4547	T4544	conj	uptake,formation
R2851	T4548	T4542	prep	of,utilization
R2852	T4549	T4548	pobj	glucose,of
R2853	T4550	T4534	punct	", ",carried
R2854	T4551	T4534	nsubj	we,carried
R2855	T4552	T4534	prt	out,carried
R2856	T4553	T4554	amod	several,assays
R2857	T4554	T4534	dobj	assays,carried
R2858	T4555	T4554	amod	physiological,assays
R2859	T4556	T4534	punct	.,carried
R286	T541	T540	amod	electrophysiological,responses
R2860	T4558	T4559	prep	In,performed
R2861	T4560	T4558	pobj	contrast,In
R2862	T4561	T4560	prep	to,contrast
R2863	T4562	T4561	pobj	mice,to
R2864	T4563	T4562	acl	placed,mice
R2865	T4564	T4563	prep	on,placed
R2866	T4565	T4566	det	a,diet
R2867	T4566	T4564	pobj	diet,on
R2868	T4567	T4566	amod	normal,diet
R2869	T4568	T4566	compound	chow,diet
R287	T542	T540	prep	of,responses
R2870	T4569	T4570	punct	(,data
R2871	T4570	T4566	parataxis	data,diet
R2872	T4571	T4572	punct	~,5
R2873	T4572	T4573	nummod	5,%
R2874	T4573	T4574	compound	%,fat
R2875	T4574	T4570	dep	fat,data
R2876	T4575	T4570	punct	;,data
R2877	T4576	T4570	amod	unpublished,data
R2878	T4577	T4570	punct	),data
R2879	T4578	T4559	punct	", ",performed
R288	T543	T544	amod	photosensory,neurons
R2880	T4579	T4580	nmod	RanBP2,mice
R2881	T4580	T4559	nsubj	mice,performed
R2882	T4581	T4579	punct	+,RanBP2
R2883	T4582	T4579	punct	/,RanBP2
R2884	T4583	T4579	punct	−,RanBP2
R2885	T4584	T4580	prep	on,mice
R2886	T4585	T4586	det	a,diet
R2887	T4586	T4584	pobj	diet,on
R2888	T4587	T4588	amod	higher,fat
R2889	T4588	T4586	compound	fat,diet
R289	T544	T542	pobj	neurons,of
R2890	T4589	T4590	punct	(,fat
R2891	T4590	T4586	parataxis	fat,diet
R2892	T4591	T4592	punct	~,10
R2893	T4592	T4593	nummod	10,%
R2894	T4593	T4590	compound	%,fat
R2895	T4594	T4590	punct	),fat
R2896	T4595	T4596	advmod	significantly,worse
R2897	T4596	T4559	advmod	worse,performed
R2898	T4597	T4559	prep	in,performed
R2899	T4598	T4599	det	the,test
R29	T260	T254	attr	protein,is
R290	T545	T543	cc	and,photosensory
R2900	T4599	T4597	pobj	test,in
R2901	T4600	T4601	compound	glucose,tolerance
R2902	T4601	T4599	compound	tolerance,test
R2903	T4602	T4559	advcl	beginning,performed
R2904	T4603	T4602	prep	at,beginning
R2905	T4604	T4605	nummod	6,mo
R2906	T4605	T4603	pobj	mo,at
R2907	T4606	T4605	prep	of,mo
R2908	T4607	T4606	pobj	age,of
R2909	T4608	T4609	punct	(,7A
R291	T546	T543	conj	postreceptoral,photosensory
R2910	T4609	T4602	parataxis	7A,beginning
R2911	T4610	T4609	compound	Figure,7A
R2912	T4611	T4609	cc	and,7A
R2913	T4612	T4609	conj	7B,7A
R2914	T4613	T4609	punct	),7A
R2915	T4614	T4559	punct	", ",performed
R2916	T4615	T4616	advmod	thus,supporting
R2917	T4616	T4559	advcl	supporting,performed
R2918	T4617	T4618	mark	that,exhibited
R2919	T4618	T4616	ccomp	exhibited,supporting
R292	T547	T533	punct	.,accompanied
R2920	T4619	T4620	det	the,mice
R2921	T4620	T4618	nsubj	mice,exhibited
R2922	T4621	T4620	nmod	RanBP2,mice
R2923	T4622	T4621	punct	+,RanBP2
R2924	T4623	T4621	punct	/,RanBP2
R2925	T4624	T4621	punct	−,RanBP2
R2926	T4625	T4626	det	a,deficit
R2927	T4626	T4618	dobj	deficit,exhibited
R2928	T4627	T4626	prep	in,deficit
R2929	T4628	T4629	compound	glucose,clearance
R293	T549	T550	advmod	Hence,emerge
R2930	T4629	T4627	pobj	clearance,in
R2931	T4630	T4559	punct	.,performed
R2932	T4632	T4633	det	This,deficit
R2933	T4633	T4634	nsubjpass	deficit,rescued
R2934	T4635	T4634	auxpass	was,rescued
R2935	T4636	T4634	prep	in,rescued
R2936	T4637	T4638	nmod	RanBP2,mice
R2937	T4638	T4636	pobj	mice,in
R2938	T4639	T4637	punct	+,RanBP2
R2939	T4640	T4637	punct	/,RanBP2
R294	T551	T550	punct	", ",emerge
R2940	T4641	T4637	punct	−,RanBP2
R2941	T4642	T4638	prep	of,mice
R2942	T4643	T4644	amod	mixed,background
R2943	T4644	T4642	pobj	background,of
R2944	T4645	T4646	compound	C57BL,129Ola
R2945	T4646	T4644	compound	129Ola,background
R2946	T4647	T4646	punct	/,129Ola
R2947	T4648	T4646	compound	6J,129Ola
R2948	T4649	T4646	punct	/,129Ola
R2949	T4650	T4651	punct	(,S3
R295	T552	T550	nsubj	RanBP2,emerge
R2950	T4651	T4634	parataxis	S3,rescued
R2951	T4652	T4651	compound	Figure,S3
R2952	T4653	T4651	punct	),S3
R2953	T4654	T4634	punct	.,rescued
R2954	T4656	T4657	compound	Glucose,clearance
R2955	T4657	T4658	nsubjpass	clearance,affected
R2956	T4659	T4658	auxpass	was,affected
R2957	T4660	T4658	neg	not,affected
R2958	T4661	T4658	prep	due,affected
R2959	T4662	T4661	pcomp	to,due
R296	T553	T552	cc	and,RanBP2
R2960	T4663	T4664	det	a,disturbance
R2961	T4664	T4661	pobj	disturbance,due
R2962	T4665	T4664	prep	in,disturbance
R2963	T4666	T4667	npadvmod	insulin,mediated
R2964	T4667	T4669	amod	mediated,uptake
R2965	T4668	T4667	punct	-,mediated
R2966	T4669	T4665	pobj	uptake,in
R2967	T4670	T4669	compound	glucose,uptake
R2968	T4671	T4672	punct	(,7C
R2969	T4672	T4658	parataxis	7C,affected
R297	T554	T555	poss	its,partners
R2970	T4673	T4672	compound	Figure,7C
R2971	T4674	T4672	punct	),7C
R2972	T4675	T4658	punct	.,affected
R2973	T4677	T4678	advmod	Then,probed
R2974	T4679	T4678	punct	", ",probed
R2975	T4680	T4678	nsubj	we,probed
R2976	T4681	T4682	mark	whether,induces
R2977	T4682	T4678	ccomp	induces,probed
R2978	T4683	T4682	nsubj	RanBP2,induces
R2979	T4684	T4682	dobj	impairment,induces
R298	T555	T552	conj	partners,RanBP2
R2980	T4685	T4684	prep	of,impairment
R2981	T4686	T4685	pobj	gluconeogenesis,of
R2982	T4687	T4684	punct	", ",impairment
R2983	T4688	T4689	dep	which,contribute
R2984	T4689	T4684	relcl	contribute,impairment
R2985	T4690	T4689	aux	could,contribute
R2986	T4691	T4689	prep	to,contribute
R2987	T4692	T4693	det	the,production
R2988	T4693	T4691	pobj	production,to
R2989	T4694	T4693	amod	pathophysiological,production
R299	T556	T550	prep	as,emerge
R2990	T4695	T4693	cc	and,production
R2991	T4696	T4693	conj	clearance,production
R2992	T4697	T4693	prep	of,production
R2993	T4698	T4697	pobj	glucose,of
R2994	T4699	T4678	punct	.,probed
R2995	T4701	T4702	prep	To,showed
R2996	T4703	T4704	det	this,end
R2997	T4704	T4701	pobj	end,To
R2998	T4705	T4702	punct	", ",showed
R2999	T4706	T4707	det	the,administration
R30	T261	T260	amod	large,protein
R300	T557	T558	amod	critical,modulators
R3000	T4707	T4702	nsubj	administration,showed
R3001	T4708	T4707	prep	of,administration
R3002	T4709	T4710	det	the,precursor
R3003	T4710	T4708	pobj	precursor,of
R3004	T4711	T4712	amod	gluconeogenic,substrate
R3005	T4712	T4710	compound	substrate,precursor
R3006	T4713	T4710	punct	", ",precursor
R3007	T4714	T4710	appos	pyruvate,precursor
R3008	T4715	T4707	punct	(,administration
R3009	T4716	T4717	compound	pyruvate,tolerance
R301	T558	T556	pobj	modulators,as
R3010	T4717	T4718	compound	tolerance,test
R3011	T4718	T4707	appos	test,administration
R3012	T4719	T4702	punct	),showed
R3013	T4720	T4702	punct	", ",showed
R3014	T4721	T4722	mark	that,was
R3015	T4722	T4702	ccomp	was,showed
R3016	T4723	T4722	expl	there,was
R3017	T4724	T4725	det	no,difference
R3018	T4725	T4722	attr	difference,was
R3019	T4726	T4725	prep	in,difference
R302	T559	T558	prep	of,modulators
R3020	T4727	T4728	compound	glucose,production
R3021	T4728	T4726	pobj	production,in
R3022	T4729	T4722	prep	in,was
R3023	T4730	T4731	nmod	RanBP2,mice
R3024	T4731	T4729	pobj	mice,in
R3025	T4732	T4730	punct	+,RanBP2
R3026	T4733	T4730	punct	/,RanBP2
R3027	T4734	T4730	punct	−,RanBP2
R3028	T4735	T4736	punct	(,7D
R3029	T4736	T4722	parataxis	7D,was
R303	T560	T561	amod	neuronal,HKI
R3030	T4737	T4736	compound	Figure,7D
R3031	T4738	T4736	punct	),7D
R3032	T4739	T4702	punct	.,showed
R3033	T4741	T4742	advmod	Hence,had
R3034	T4743	T4742	punct	", ",had
R3035	T4744	T4745	amod	partial,loss
R3036	T4745	T4742	nsubj	loss,had
R3037	T4746	T4745	punct	-,loss
R3038	T4747	T4745	prep	of,loss
R3039	T4748	T4747	punct	-,of
R304	T561	T559	pobj	HKI,of
R3040	T4749	T4747	pobj	function,of
R3041	T4750	T4745	prep	of,loss
R3042	T4751	T4750	pobj	RanBP2,of
R3043	T4752	T4753	det	no,impact
R3044	T4753	T4742	dobj	impact,had
R3045	T4754	T4753	prep	on,impact
R3046	T4755	T4756	det	the,pathway
R3047	T4756	T4754	pobj	pathway,on
R3048	T4757	T4756	compound	gluconeogenesis,pathway
R3049	T4758	T4742	punct	.,had
R305	T562	T561	punct	", ",HKI
R3050	T4760	T4761	advmod	However,were
R3051	T4762	T4761	punct	", ",were
R3052	T4763	T4761	prep	upon,were
R3053	T4764	T4765	compound	glucose,production
R3054	T4765	T4763	pobj	production,upon
R3055	T4766	T4767	punct	(,min
R3056	T4767	T4765	parataxis	min,production
R3057	T4768	T4767	nummod	15,min
R3058	T4769	T4767	punct	),min
R3059	T4770	T4761	punct	", ",were
R306	T563	T564	compound	glucose,catabolism
R3060	T4771	T4772	det	the,rates
R3061	T4772	T4761	nsubj	rates,were
R3062	T4773	T4772	compound	clearance,rates
R3063	T4774	T4772	prep	of,rates
R3064	T4775	T4774	pobj	glucose,of
R3065	T4776	T4761	advmod	again,were
R3066	T4777	T4778	advmod	significantly,slower
R3067	T4778	T4761	acomp	slower,were
R3068	T4779	T4761	prep	in,were
R3069	T4780	T4779	pobj	RanBP2,in
R307	T564	T561	conj	catabolism,HKI
R3070	T4781	T4780	punct	+,RanBP2
R3071	T4782	T4780	punct	/,RanBP2
R3072	T4783	T4780	punct	−,RanBP2
R3073	T4784	T4761	prep	than,were
R3074	T4785	T4784	prep	in,than
R3075	T4786	T4787	nmod	RanBP2,mice
R3076	T4787	T4785	pobj	mice,in
R3077	T4788	T4786	punct	+,RanBP2
R3078	T4789	T4786	punct	/,RanBP2
R3079	T4790	T4786	punct	+,RanBP2
R308	T565	T564	punct	", ",catabolism
R3080	T4791	T4792	punct	(,7D
R3081	T4792	T4787	parataxis	7D,mice
R3082	T4793	T4792	compound	Figure,7D
R3083	T4794	T4792	punct	),7D
R3084	T4795	T4761	punct	", ",were
R3085	T4796	T4761	advcl	confirming,were
R3086	T4797	T4798	det	an,impairment
R3087	T4798	T4796	dobj	impairment,confirming
R3088	T4799	T4798	prep	in,impairment
R3089	T4800	T4801	compound	glucose,breakdown
R309	T566	T567	compound	energy,homeostasis
R3090	T4801	T4799	pobj	breakdown,in
R3091	T4802	T4761	punct	.,were
R31	T262	T260	amod	multimodular,protein
R310	T567	T564	conj	homeostasis,catabolism
R3106	T5002	T5003	nsubj	Haploinsufficiency,Causes
R3107	T5004	T5002	prep	of,Haploinsufficiency
R3108	T5005	T5004	pobj	RanBP2,of
R3109	T5006	T5003	dobj	Deficits,Causes
R311	T568	T567	punct	", ",homeostasis
R3110	T5007	T5003	prep	in,Causes
R3111	T5008	T5009	det	the,Output
R3112	T5009	T5007	pobj	Output,in
R3113	T5010	T5009	amod	Electrophysiological,Output
R3114	T5011	T5009	prep	of,Output
R3115	T5012	T5013	amod	Receptoral,Neurons
R3116	T5013	T5011	pobj	Neurons,of
R3117	T5014	T5012	cc	and,Receptoral
R3118	T5015	T5012	conj	Postreceptoral,Receptoral
R3119	T5016	T5013	amod	Retinal,Neurons
R312	T569	T567	cc	and,homeostasis
R3120	T5018	T5019	prep	In,probed
R3121	T5020	T5018	pobj	light,In
R3122	T5021	T5020	prep	of,light
R3123	T5022	T5023	det	the,expression
R3124	T5023	T5021	pobj	expression,of
R3125	T5024	T5023	amod	prominent,expression
R3126	T5025	T5023	prep	of,expression
R3127	T5026	T5025	pobj	RanBP2,of
R3128	T5027	T5026	cc	and,RanBP2
R3129	T5028	T5026	conj	HKI,RanBP2
R313	T570	T567	conj	targets,homeostasis
R3130	T5029	T5023	prep	in,expression
R3131	T5030	T5031	amod	retinal,neurons
R3132	T5031	T5029	pobj	neurons,in
R3133	T5032	T5033	punct	[,19
R3134	T5033	T5023	parataxis	19,expression
R3135	T5034	T5033	nummod	1,19
R3136	T5035	T5033	punct	",",19
R3137	T5036	T5033	punct	],19
R3138	T5037	T5023	punct	", ",expression
R3139	T5038	T5039	det	the,dependence
R314	T571	T570	prep	for,targets
R3140	T5039	T5023	conj	dependence,expression
R3141	T5040	T5039	amod	vital,dependence
R3142	T5041	T5039	prep	of,dependence
R3143	T5042	T5043	det	the,retina
R3144	T5043	T5041	pobj	retina,of
R3145	T5044	T5043	amod	neuronal,retina
R3146	T5045	T5043	punct	(,retina
R3147	T5046	T5043	cc	and,retina
R3148	T5047	T5043	conj	brain,retina
R3149	T5048	T5039	punct	),dependence
R315	T572	T573	amod	metabolic,disorders
R3150	T5049	T5039	prep	on,dependence
R3151	T5050	T5049	pobj	glucose,on
R3152	T5051	T5039	prep	as,dependence
R3153	T5052	T5053	det	the,source
R3154	T5053	T5051	pobj	source,as
R3155	T5054	T5053	amod	main,source
R3156	T5055	T5053	compound	substrate,source
R3157	T5056	T5053	prep	for,source
R3158	T5057	T5058	compound	energy,production
R3159	T5058	T5056	pobj	production,for
R316	T573	T571	pobj	disorders,for
R3160	T5059	T5039	punct	", ",dependence
R3161	T5060	T5039	cc	and,dependence
R3162	T5061	T5062	det	the,impact
R3163	T5062	T5039	conj	impact,dependence
R3164	T5063	T5062	amod	determinant,impact
R3165	T5064	T5062	prep	of,impact
R3166	T5065	T5066	amod	metabolic,disorders
R3167	T5066	T5064	pobj	disorders,of
R3168	T5067	T5066	punct	", ",disorders
R3169	T5068	T5066	prep	such,disorders
R317	T574	T573	punct	", ",disorders
R3170	T5069	T5068	pcomp	as,such
R3171	T5070	T5068	pobj	diabetes,such
R3172	T5071	T5062	punct	", ",impact
R3173	T5072	T5062	prep	in,impact
R3174	T5073	T5074	amod	retinal,function
R3175	T5074	T5072	pobj	function,in
R3176	T5075	T5062	punct	(,impact
R3177	T5076	T5077	advmod	e.g.,retinopathy
R3178	T5077	T5062	appos	retinopathy,impact
R3179	T5078	T5077	punct	", ",retinopathy
R318	T575	T573	compound	aging,disorders
R3180	T5079	T5077	amod	diabetic,retinopathy
R3181	T5080	T5062	punct	),impact
R3182	T5081	T5082	punct	[,37
R3183	T5082	T5062	parataxis	37,impact
R3184	T5083	T5082	punct	],37
R3185	T5084	T5019	punct	", ",probed
R3186	T5085	T5019	nsubj	we,probed
R3187	T5086	T5087	det	the,impact
R3188	T5087	T5019	dobj	impact,probed
R3189	T5088	T5087	prep	of,impact
R319	T576	T573	cc	and,disorders
R3190	T5089	T5088	pobj	deficits,of
R3191	T5090	T5089	prep	in,deficits
R3192	T5091	T5090	pobj	RanBP2,in
R3193	T5092	T5091	punct	", ",RanBP2
R3194	T5093	T5091	conj	HKI,RanBP2
R3195	T5094	T5093	punct	", ",HKI
R3196	T5095	T5093	cc	and,HKI
R3197	T5096	T5093	conj	ATP,HKI
R3198	T5097	T5087	punct	", ",impact
R3199	T5098	T5087	prep	on,impact
R32	T263	T262	cc	and,multimodular
R320	T577	T578	amod	allied,neuropathies
R3200	T5099	T5100	det	the,responses
R3201	T5100	T5098	pobj	responses,on
R3202	T5101	T5100	amod	electrophysiological,responses
R3203	T5102	T5100	prep	of,responses
R3204	T5103	T5104	nmod	subclasses,neurons
R3205	T5104	T5102	pobj	neurons,of
R3206	T5105	T5103	punct	(,subclasses
R3207	T5106	T5103	appos	rod,subclasses
R3208	T5107	T5106	cc	and,rod
R3209	T5108	T5106	conj	cone,rod
R321	T578	T573	conj	neuropathies,disorders
R3210	T5109	T5103	punct	),subclasses
R3211	T5110	T5104	nmod	photoreceptor,neurons
R3212	T5111	T5110	cc	and,photoreceptor
R3213	T5112	T5110	conj	postreceptor,photoreceptor
R3214	T5113	T5104	amod	retinal,neurons
R3215	T5114	T5104	prep	of,neurons
R3216	T5115	T5114	pobj	RanBP2,of
R3217	T5116	T5115	punct	+,RanBP2
R3218	T5117	T5115	punct	/,RanBP2
R3219	T5118	T5115	punct	−,RanBP2
R322	T579	T550	punct	.,emerge
R3220	T5119	T5098	cc	and,on
R3221	T5120	T5098	conj	in,on
R3222	T5121	T5122	nmod	RanBP2,mice
R3223	T5122	T5120	pobj	mice,in
R3224	T5123	T5121	punct	+,RanBP2
R3225	T5124	T5121	punct	/,RanBP2
R3226	T5125	T5121	punct	+,RanBP2
R3227	T5126	T5019	punct	.,probed
R3228	T5128	T5129	det	The,responses
R3229	T5129	T5136	nsubjpass	responses,reduced
R323	T231	T232	nsubj	RanBP2,Modulates
R3230	T5130	T5129	amod	scotopic,responses
R3231	T5131	T5132	punct	(,adapted
R3232	T5132	T5130	parataxis	adapted,scotopic
R3233	T5133	T5132	npadvmod	dark,adapted
R3234	T5134	T5132	punct	-,adapted
R3235	T5135	T5132	punct	),adapted
R3236	T5137	T5129	acl	mediated,responses
R3237	T5138	T5137	agent	by,mediated
R3238	T5139	T5140	det	the,pathway
R3239	T5140	T5138	pobj	pathway,by
R3240	T5141	T5142	compound	rod,photoreceptor
R3241	T5142	T5140	compound	photoreceptor,pathway
R3242	T5143	T5140	prep	at,pathway
R3243	T5144	T5145	amod	low,stimulus
R3244	T5145	T5147	compound	stimulus,intensities
R3245	T5146	T5145	punct	-,stimulus
R3246	T5147	T5143	pobj	intensities,at
R3247	T5148	T5140	cc	and,pathway
R3248	T5149	T5150	amod	mixed,pathways
R3249	T5150	T5140	conj	pathways,pathway
R3250	T5151	T5150	nmod	rod,pathways
R3251	T5152	T5151	cc	and,rod
R3252	T5153	T5151	conj	cone,rod
R3253	T5154	T5150	prep	at,pathways
R3254	T5155	T5156	amod	high,stimulus
R3255	T5156	T5158	compound	stimulus,intensities
R3256	T5157	T5156	punct	-,stimulus
R3257	T5158	T5154	pobj	intensities,at
R3258	T5159	T5136	auxpass	were,reduced
R3259	T5160	T5136	advmod	substantially,reduced
R3260	T5161	T5136	prep	in,reduced
R3261	T5162	T5163	nmod	RanBP2,mice
R3262	T5163	T5161	pobj	mice,in
R3263	T5164	T5162	punct	+,RanBP2
R3264	T5165	T5162	punct	/,RanBP2
R3265	T5166	T5162	punct	−,RanBP2
R3266	T5167	T5168	punct	(,8A
R3267	T5168	T5136	parataxis	8A,reduced
R3268	T5169	T5168	compound	Figure,8A
R3269	T5170	T5168	punct	),8A
R3270	T5171	T5136	punct	.,reduced
R3271	T5173	T5174	det	The,differences
R3272	T5174	T5175	nsubj	differences,were
R3273	T5176	T5174	prep	in,differences
R3274	T5177	T5178	det	the,responses
R3275	T5178	T5176	pobj	responses,in
R3276	T5179	T5178	amod	photopic,responses
R3277	T5180	T5181	punct	(,adapted
R3278	T5181	T5179	parataxis	adapted,photopic
R3279	T5182	T5181	npadvmod	light,adapted
R3280	T5183	T5181	punct	-,adapted
R3281	T5184	T5181	punct	),adapted
R3282	T5185	T5178	punct	", ",responses
R3283	T5186	T5178	acl	initiated,responses
R3284	T5187	T5186	agent	by,initiated
R3285	T5188	T5189	compound	cone,photoreceptors
R3286	T5189	T5187	pobj	photoreceptors,by
R3287	T5190	T5189	punct	", ",photoreceptors
R3288	T5191	T5192	dep	which,make
R3289	T5192	T5189	relcl	make,photoreceptors
R3290	T5193	T5192	prt	up,make
R3291	T5194	T5195	nummod	3,%
R3292	T5195	T5192	dobj	%,make
R3293	T5196	T5195	prep	of,%
R3294	T5197	T5198	det	the,neurons
R3295	T5198	T5196	pobj	neurons,of
R3296	T5199	T5198	amod	photosensory,neurons
R3297	T5200	T5198	prep	in,neurons
R3298	T5201	T5202	det	the,retina
R3299	T5202	T5200	pobj	retina,in
R33	T264	T262	conj	pleiotropic,multimodular
R3300	T5203	T5202	compound	mouse,retina
R3301	T5204	T5205	punct	[,38
R3302	T5205	T5174	parataxis	38,differences
R3303	T5206	T5205	punct	],38
R3304	T5207	T5175	punct	", ",were
R3305	T5208	T5209	advmod	less,obvious
R3306	T5209	T5175	acomp	obvious,were
R3307	T5210	T5175	cc	but,were
R3308	T5211	T5212	advmod	still,exhibited
R3309	T5212	T5175	conj	exhibited,were
R3310	T5213	T5214	det	a,trend
R3311	T5214	T5212	dobj	trend,exhibited
R3312	T5215	T5214	prep	toward,trend
R3313	T5216	T5217	amod	reduced,amplitudes
R3314	T5217	T5215	pobj	amplitudes,toward
R3315	T5218	T5214	prep	across,trend
R3316	T5219	T5220	det	a,range
R3317	T5220	T5218	pobj	range,across
R3318	T5221	T5220	prep	of,range
R3319	T5222	T5223	amod	increasing,intensities
R3320	T5223	T5221	pobj	intensities,of
R3321	T5224	T5225	amod	light,stimulus
R3322	T5225	T5223	compound	stimulus,intensities
R3323	T5226	T5227	punct	(,8B
R3324	T5227	T5212	parataxis	8B,exhibited
R3325	T5228	T5227	compound	Figure,8B
R3326	T5229	T5227	punct	),8B
R3327	T5230	T5175	punct	.,were
R3328	T5232	T5233	det	The,reduction
R3329	T5233	T5234	nsubj	reduction,included
R3330	T5235	T5233	prep	in,reduction
R3331	T5236	T5237	det	the,responses
R3332	T5237	T5235	pobj	responses,in
R3333	T5238	T5237	amod	scotopic,responses
R3334	T5239	T5234	dobj	decreases,included
R3335	T5240	T5239	prep	in,decreases
R3336	T5241	T5242	preconj	both,wave
R3337	T5242	T5245	nmod	wave,amplitudes
R3338	T5243	T5242	nmod	b,wave
R3339	T5244	T5242	punct	-,wave
R3340	T5245	T5240	pobj	amplitudes,in
R3341	T5246	T5247	punct	(,8C
R3342	T5247	T5242	parataxis	8C,wave
R3343	T5248	T5247	compound	Figure,8C
R3344	T5249	T5247	punct	),8C
R3345	T5250	T5242	cc	and,wave
R3346	T5251	T5252	compound	a,wave
R3347	T5252	T5242	conj	wave,wave
R3348	T5253	T5252	punct	-,wave
R3349	T5254	T5255	punct	(,8D
R3350	T5255	T5252	parataxis	8D,wave
R3351	T5256	T5255	compound	Figure,8D
R3352	T5257	T5255	punct	),8D
R3353	T5258	T5245	acl	mediated,amplitudes
R3354	T5259	T5258	agent	by,mediated
R3355	T5260	T5261	amod	postreceptoral,neurons
R3356	T5261	T5259	pobj	neurons,by
R3357	T5262	T5260	cc	and,postreceptoral
R3358	T5263	T5260	conj	receptoral,postreceptoral
R3359	T5264	T5234	punct	", ",included
R3360	T5265	T5234	advmod	respectively,included
R3361	T5266	T5234	punct	.,included
R3362	T5268	T5269	amod	Postreceptoral,responses
R3363	T5269	T5274	nsubj	responses,tended
R3364	T5270	T5271	amod	second,order
R3365	T5271	T5269	compound	order,responses
R3366	T5272	T5271	punct	-,order
R3367	T5273	T5269	compound	neuron,responses
R3368	T5275	T5269	punct	", ",responses
R3369	T5276	T5269	acl	represented,responses
R3370	T5277	T5276	agent	by,represented
R3371	T5278	T5279	det	the,wave
R3372	T5279	T5277	pobj	wave,by
R3373	T5280	T5279	compound	b,wave
R3374	T5281	T5279	punct	-,wave
R3375	T5282	T5274	punct	", ",tended
R3376	T5283	T5284	aux	to,be
R3377	T5284	T5274	xcomp	be,tended
R3378	T5285	T5286	advmod	more,reduced
R3379	T5286	T5284	acomp	reduced,be
R338	T1039	T1040	det	The,Nup358
R3380	T5287	T5285	advmod	consistently,more
R3381	T5288	T5287	cc	and,consistently
R3382	T5289	T5287	conj	substantially,consistently
R3383	T5290	T5286	prep	than,reduced
R3384	T5291	T5292	det	the,waves
R3385	T5292	T5290	pobj	waves,than
R3386	T5293	T5292	compound	a,waves
R3387	T5294	T5292	punct	-,waves
R3388	T5295	T5292	punct	", ",waves
R3389	T5296	T5297	dep	which,reflect
R339	T1040	T1043	nsubj	Nup358,is
R3390	T5297	T5292	relcl	reflect,waves
R3391	T5298	T5297	advmod	directly,reflect
R3392	T5299	T5300	compound	photoreceptor,activity
R3393	T5300	T5297	dobj	activity,reflect
R3394	T5301	T5274	punct	.,tended
R3395	T5303	T5304	mark	Since,depend
R3396	T5304	T5310	advcl	depend,suggests
R3397	T5305	T5306	amod	second,order
R3398	T5306	T5308	compound	order,responses
R3399	T5307	T5306	punct	-,order
R34	T265	T254	punct	.,is
R340	T1041	T1040	compound	RanBP2,Nup358
R3400	T5308	T5304	nsubj	responses,depend
R3401	T5309	T5308	compound	neuron,responses
R3402	T5311	T5304	prep	on,depend
R3403	T5312	T5311	pobj	input,on
R3404	T5313	T5312	prep	from,input
R3405	T5314	T5313	pobj	photoreceptors,from
R3406	T5315	T5310	punct	", ",suggests
R3407	T5316	T5310	nsubj	this,suggests
R3408	T5317	T5318	mark	that,are
R3409	T5318	T5310	ccomp	are,suggests
R341	T1042	T1040	punct	/,Nup358
R3410	T5319	T5320	amod	reduced,amplitudes
R3411	T5320	T5318	nsubj	amplitudes,are
R3412	T5321	T5322	compound	b,wave
R3413	T5322	T5320	compound	wave,amplitudes
R3414	T5323	T5322	punct	-,wave
R3415	T5324	T5325	det	the,result
R3416	T5325	T5318	attr	result,are
R3417	T5326	T5325	prep	of,result
R3418	T5327	T5328	det	the,accumulation
R3419	T5328	T5326	pobj	accumulation,of
R342	T1044	T1045	det	a,protein
R3420	T5329	T5328	prep	of,accumulation
R3421	T5330	T5329	pobj	decreases,of
R3422	T5331	T5330	prep	in,decreases
R3423	T5332	T5333	det	the,response
R3424	T5333	T5331	pobj	response,in
R3425	T5334	T5333	amod	light,response
R3426	T5335	T5333	prep	of,response
R3427	T5336	T5337	preconj	both,photoreceptors
R3428	T5337	T5335	pobj	photoreceptors,of
R3429	T5338	T5337	cc	and,photoreceptors
R343	T1045	T1043	attr	protein,is
R3430	T5339	T5340	amod	postreceptoral,neurons
R3431	T5340	T5337	conj	neurons,photoreceptors
R3432	T5341	T5310	punct	.,suggests
R3433	T5343	T5344	nsubj	Anesthetics,cause
R3434	T5345	T5343	punct	", ",Anesthetics
R3435	T5346	T5347	advmod	particularly,ketamine
R3436	T5347	T5343	appos	ketamine,Anesthetics
R3437	T5348	T5344	punct	", ",cause
R3438	T5349	T5344	aux	can,cause
R3439	T5350	T5351	amod	sustained,elevation
R344	T1046	T1045	amod	unique,protein
R3440	T5351	T5344	dobj	elevation,cause
R3441	T5352	T5351	prep	of,elevation
R3442	T5353	T5352	pobj	glucose,of
R3443	T5354	T5351	prep	in,elevation
R3444	T5355	T5354	pobj	mice,in
R3445	T5356	T5351	punct	", ",elevation
R3446	T5357	T5358	dep	which,affects
R3447	T5358	T5351	relcl	affects,elevation
R3448	T5359	T5358	prep	in,affects
R3449	T5360	T5359	pobj	turn,in
R345	T1047	T1045	nmod	vertebrate,protein
R3450	T5361	T5362	compound	electroretinogram,responses
R3451	T5362	T5358	dobj	responses,affects
R3452	T5363	T5364	punct	[,39
R3453	T5364	T5358	parataxis	39,affects
R3454	T5365	T5364	punct	],39
R3455	T5366	T5344	punct	.,cause
R3456	T5368	T5369	advmod	Thus,were
R3457	T5370	T5369	punct	", ",were
R3458	T5371	T5369	nsubj	we,were
R3459	T5372	T5369	acomp	concerned,were
R346	T1048	T1047	cc	and,vertebrate
R3460	T5373	T5374	mark	that,reflect
R3461	T5374	T5372	ccomp	reflect,concerned
R3462	T5375	T5374	nsubj	differences,reflect
R3463	T5376	T5375	prep	in,differences
R3464	T5377	T5378	compound	electroretinogram,amplitudes
R3465	T5378	T5376	pobj	amplitudes,in
R3466	T5379	T5375	prep	between,differences
R3467	T5380	T5379	pobj	RanBP2,between
R3468	T5381	T5380	punct	+,RanBP2
R3469	T5382	T5380	punct	/,RanBP2
R347	T1049	T1050	amod	large,scaffold
R3470	T5383	T5380	punct	−,RanBP2
R3471	T5384	T5380	cc	and,RanBP2
R3472	T5385	T5380	conj	RanBP2,RanBP2
R3473	T5386	T5385	punct	+,RanBP2
R3474	T5387	T5385	punct	/,RanBP2
R3475	T5388	T5385	punct	+,RanBP2
R3476	T5389	T5374	aux	may,reflect
R3477	T5390	T5374	dobj	differences,reflect
R3478	T5391	T5390	prep	in,differences
R3479	T5392	T5393	compound	glucose,level
R348	T1050	T1047	conj	scaffold,vertebrate
R3480	T5393	T5394	compound	level,changes
R3481	T5394	T5391	pobj	changes,in
R3482	T5395	T5394	prep	in,changes
R3483	T5396	T5395	pobj	response,in
R3484	T5397	T5396	prep	to,response
R3485	T5398	T5397	pobj	anesthesia,to
R3486	T5399	T5369	punct	.,were
R3487	T5401	T5402	advmod	However,found
R3488	T5403	T5402	punct	", ",found
R3489	T5404	T5402	nsubj	we,found
R349	T1051	T1045	acl	comprised,protein
R3490	T5405	T5406	det	no,differences
R3491	T5406	T5402	dobj	differences,found
R3492	T5407	T5406	amod	significant,differences
R3493	T5408	T5406	prep	in,differences
R3494	T5409	T5410	compound	glucose,levels
R3495	T5410	T5408	pobj	levels,in
R3496	T5411	T5410	acl	measured,levels
R3497	T5412	T5411	prep	before,measured
R3498	T5413	T5411	cc	and,measured
R3499	T5414	T5415	det	every,min
R35	T267	T268	amod	Several,partners
R350	T1052	T1051	prep	of,comprised
R3500	T5415	T5417	npadvmod	min,during
R3501	T5416	T5415	nummod	15,min
R3502	T5417	T5418	prep	during,min
R3503	T5418	T5411	conj	min,measured
R3504	T5419	T5418	nummod	75,min
R3505	T5420	T5418	prep	of,min
R3506	T5421	T5420	pobj	anesthesia,of
R3507	T5422	T5423	punct	(,5
R3508	T5423	T5402	parataxis	5,found
R3509	T5424	T5423	nsubj	n,5
R351	T1053	T1054	amod	multiple,domains
R3510	T5425	T5423	punct	=,5
R3511	T5426	T5423	quantmod	4,5
R3512	T5427	T5423	punct	–,5
R3513	T5428	T5423	punct	),5
R3514	T5429	T5402	punct	.,found
R3515	T5431	T5432	nsubj	Glucose,rose
R3516	T5433	T5432	prep	at,rose
R3517	T5434	T5435	det	the,rate
R3518	T5435	T5433	pobj	rate,at
R3519	T5436	T5435	amod	same,rate
R352	T1054	T1052	pobj	domains,of
R3520	T5437	T5432	cc	and,rose
R3521	T5438	T5432	conj	reached,rose
R3522	T5439	T5440	det	a,maximum
R3523	T5440	T5438	dobj	maximum,reached
R3524	T5441	T5440	prep	of,maximum
R3525	T5442	T5443	advmod	approximately,3.3
R3526	T5443	T5444	nummod	3.3,level
R3527	T5444	T5441	pobj	level,of
R3528	T5445	T5443	quantmod	times,3.3
R3529	T5446	T5444	det	the,level
R353	T1055	T1054	amod	structural,domains
R3530	T5447	T5444	amod	pre-anesthesia,level
R3531	T5448	T5438	prep	in,reached
R3532	T5449	T5450	det	both,genotypes
R3533	T5450	T5448	pobj	genotypes,in
R3534	T5451	T5452	punct	(,data
R3535	T5452	T5438	meta	data,reached
R3536	T5453	T5452	amod	unpublished,data
R3537	T5454	T5452	punct	),data
R3538	T5455	T5432	punct	.,rose
R354	T1056	T1055	cc	and,structural
R355	T1057	T1055	conj	functional,structural
R3551	T6106	T6107	poss	Our,findings
R3552	T6107	T6108	nsubj	findings,support
R3553	T6109	T6110	mark	that,plays
R3554	T6110	T6108	ccomp	plays,support
R3555	T6111	T6110	nsubj	RanBP2,plays
R3556	T6112	T6113	det	a,role
R3557	T6113	T6110	dobj	role,plays
R3558	T6114	T6113	amod	determinant,role
R3559	T6115	T6110	prep	in,plays
R356	T1058	T1059	punct	[,1
R3560	T6116	T6115	pcomp	modulating,in
R3561	T6117	T6116	dobj	glucose,modulating
R3562	T6118	T6117	cc	and,glucose
R3563	T6119	T6120	compound	energy,homeostasis
R3564	T6120	T6117	conj	homeostasis,glucose
R3565	T6121	T6108	punct	.,support
R3566	T6123	T6124	det	The,data
R3567	T6124	T6125	nsubj	data,support
R3568	T6126	T6127	mark	that,are
R3569	T6127	T6125	ccomp	are,support
R357	T1059	T1043	parataxis	1,is
R3570	T6128	T6127	nsubj	Cox1,are
R3571	T6129	T6128	cc	and,Cox1
R3572	T6130	T6128	conj	HKI,Cox1
R3573	T6131	T6132	amod	novel,partners
R3574	T6132	T6127	attr	partners,are
R3575	T6133	T6134	advmod	in,vivo
R3576	T6134	T6127	advmod	vivo,are
R3577	T6135	T6127	prep	for,are
R3578	T6136	T6137	det	the,LD
R3579	T6137	T6135	pobj	LD,for
R358	T1060	T1061	punct	–,4
R3580	T6138	T6137	amod	large,LD
R3581	T6139	T6137	prep	of,LD
R3582	T6140	T6139	pobj	RanBP2,of
R3583	T6141	T6142	punct	(,1
R3584	T6142	T6127	parataxis	1,are
R3585	T6143	T6142	nmod	Figures,1
R3586	T6144	T6142	cc	and,1
R3587	T6145	T6142	conj	2,1
R3588	T6146	T6142	punct	),1
R3589	T6147	T6125	punct	.,support
R359	T1061	T1059	prep	4,1
R3590	T6149	T6150	det	The,LD
R3591	T6150	T6151	nsubj	LD,exhibits
R3592	T6152	T6150	prep	of,LD
R3593	T6153	T6152	pobj	RanBP2,of
R3594	T6154	T6155	compound	chaperone,activity
R3595	T6155	T6151	dobj	activity,exhibits
R3596	T6156	T6155	prep	toward,activity
R3597	T6157	T6158	compound	folding,intermediates
R3598	T6158	T6156	pobj	intermediates,toward
R3599	T6159	T6158	prep	of,intermediates
R36	T268	T270	nsubjpass	partners,identified
R360	T1062	T1059	punct	],1
R3600	T6160	T6159	pobj	Cox11,of
R3601	T6161	T6158	punct	", ",intermediates
R3602	T6162	T6158	cc	and,intermediates
R3603	T6163	T6164	advmod	possibly,HKI
R3604	T6164	T6158	conj	HKI,intermediates
R3605	T6165	T6164	punct	", ",HKI
R3606	T6166	T6164	det	the,HKI
R3607	T6167	T6164	amod	mature,HKI
R3608	T6168	T6169	punct	(,Figure
R3609	T6169	T6151	parataxis	Figure,exhibits
R361	T1063	T1043	punct	.,is
R3610	T6170	T6169	nummod	1,Figure
R3611	T6171	T6169	punct	),Figure
R3612	T6172	T6151	punct	.,exhibits
R3613	T6174	T6175	nsubj	Cox11,inhibits
R3614	T6176	T6175	advmod	noncompetitively,inhibits
R3615	T6177	T6178	det	the,activity
R3616	T6178	T6175	dobj	activity,inhibits
R3617	T6179	T6178	prep	of,activity
R3618	T6180	T6179	pobj	HKI,of
R3619	T6181	T6182	mark	with,required
R362	T1065	T1066	amod	Several,roles
R3620	T6182	T6175	advcl	required,inhibits
R3621	T6183	T6184	punct	~,3
R3622	T6184	T6187	nummod	3,molecules
R3623	T6185	T6184	quantmod	2,3
R3624	T6186	T6184	punct	–,3
R3625	T6187	T6182	nsubj	molecules,required
R3626	T6188	T6187	prep	of,molecules
R3627	T6189	T6188	pobj	Cox11,of
R3628	T6190	T6191	punct	(,assuming
R3629	T6191	T6187	parataxis	assuming,molecules
R363	T1066	T1067	nsubj	roles,emerged
R3630	T6192	T6191	dobj	formation,assuming
R3631	T6193	T6192	prep	of,formation
R3632	T6194	T6195	compound	Cox11,dimer
R3633	T6195	T6193	pobj	dimer,of
R3634	T6196	T6191	punct	),assuming
R3635	T6197	T6198	aux	to,inhibit
R3636	T6198	T6182	advcl	inhibit,required
R3637	T6199	T6198	advmod	completely,inhibit
R3638	T6200	T6201	det	the,activity
R3639	T6201	T6198	dobj	activity,inhibit
R364	T1068	T1066	prep	of,roles
R3640	T6202	T6201	prep	of,activity
R3641	T6203	T6204	det	a,molecule
R3642	T6204	T6202	pobj	molecule,of
R3643	T6205	T6204	prep	of,molecule
R3644	T6206	T6205	pobj	HKI,of
R3645	T6207	T6208	punct	(,2A
R3646	T6208	T6175	parataxis	2A,inhibits
R3647	T6209	T6208	compound	Figure,2A
R3648	T6210	T6208	cc	and,2A
R3649	T6211	T6208	conj	2B,2A
R365	T1069	T1068	pobj	RanBP2,of
R3650	T6212	T6208	punct	),2A
R3651	T6213	T6175	punct	.,inhibits
R3652	T6215	T6216	nsubj	Cox11,sequesters
R3653	T6217	T6216	dobj	HKI,sequesters
R3654	T6218	T6216	prep	by,sequesters
R3655	T6219	T6218	pcomp	binding,by
R3656	T6220	T6219	prep	to,binding
R3657	T6221	T6220	pobj	HKI,to
R3658	T6222	T6219	prep	at,binding
R3659	T6223	T6224	det	a,site
R366	T1070	T1067	aux	have,emerged
R3660	T6224	T6222	pobj	site,at
R3661	T6225	T6226	dep	that,is
R3662	T6226	T6224	relcl	is,site
R3663	T6227	T6226	acomp	distinct,is
R3664	T6228	T6227	prep	from,distinct
R3665	T6229	T6230	det	the,site
R3666	T6230	T6228	pobj	site,from
R3667	T6231	T6230	amod	active,site
R3668	T6232	T6226	cc	and,is
R3669	T6233	T6234	advmod	effectively,reduces
R367	T1071	T1072	dep	that,implicate
R3670	T6234	T6226	conj	reduces,is
R3671	T6235	T6236	det	the,availability
R3672	T6236	T6234	dobj	availability,reduces
R3673	T6237	T6236	prep	of,availability
R3674	T6238	T6239	punct	[,tot
R3675	T6239	T6237	pobj	tot,of
R3676	T6240	T6239	nmod	HKI,tot
R3677	T6241	T6239	punct	],tot
R3678	T6242	T6236	prep	for,availability
R3679	T6243	T6242	pobj	catalysis,for
R368	T1072	T1067	ccomp	implicate,emerged
R3680	T6244	T6216	punct	.,sequesters
R3681	T6246	T6247	nsubjpass	This,reflected
R3682	T6248	T6247	auxpass	is,reflected
R3683	T6249	T6247	agent	by,reflected
R3684	T6250	T6251	det	a,reduction
R3685	T6251	T6249	pobj	reduction,by
R3686	T6252	T6251	prep	of,reduction
R3687	T6253	T6254	det	the,V max
R3688	T6254	T6252	pobj	V max,of
R3689	T6255	T6254	prep	of,V max
R369	T1073	T1072	dobj	RanBP2,implicate
R3690	T6256	T6255	pobj	HKI,of
R3691	T6257	T6251	prep	without,reduction
R3692	T6258	T6259	advmod	significantly,affecting
R3693	T6259	T6257	pcomp	affecting,without
R3694	T6260	T6261	det	the,K m
R3695	T6261	T6259	dobj	K m,affecting
R3696	T6262	T6261	prep	of,K m
R3697	T6263	T6264	det	the,site
R3698	T6264	T6262	pobj	site,of
R3699	T6265	T6264	amod	active,site
R37	T269	T268	amod	molecular,partners
R370	T1074	T1072	prep	in,implicate
R3700	T6266	T6264	prep	of,site
R3701	T6267	T6266	pobj	HKI,of
R3702	T6268	T6261	prep	toward,K m
R3703	T6269	T6268	pobj	glucose,toward
R3704	T6270	T6271	punct	(,2A
R3705	T6271	T6247	parataxis	2A,reflected
R3706	T6272	T6271	compound	Figure,2A
R3707	T6273	T6271	cc	and,2A
R3708	T6274	T6271	conj	2B,2A
R3709	T6275	T6271	punct	),2A
R371	T1075	T1076	amod	nucleocytoplasmic,trafficking
R3710	T6276	T6247	punct	.,reflected
R3711	T6278	T6279	det	The,property
R3712	T6279	T6281	nsubjpass	property,suppressed
R3713	T6280	T6279	amod	inhibitory,property
R3714	T6282	T6279	prep	of,property
R3715	T6283	T6282	pobj	Cox11,of
R3716	T6284	T6279	prep	over,property
R3717	T6285	T6284	pobj	HKI,over
R3718	T6286	T6281	auxpass	is,suppressed
R3719	T6287	T6281	agent	by,suppressed
R372	T1076	T1074	pobj	trafficking,in
R3720	T6288	T6287	pobj	RanBP2,by
R3721	T6289	T6290	punct	(,2C
R3722	T6290	T6288	parataxis	2C,RanBP2
R3723	T6291	T6290	compound	Figure,2C
R3724	T6292	T6290	punct	),2C
R3725	T6293	T6288	punct	", ",RanBP2
R3726	T6294	T6295	dep	which,has
R3727	T6295	T6288	relcl	has,RanBP2
R3728	T6296	T6295	prep	by,has
R3729	T6297	T6296	pobj	itself,by
R373	T1077	T1078	punct	[,5
R3730	T6298	T6299	det	a,activity
R3731	T6299	T6295	dobj	activity,has
R3732	T6300	T6299	amod	weak,activity
R3733	T6301	T6300	cc	but,weak
R3734	T6302	T6300	conj	significant,weak
R3735	T6303	T6299	amod	inhibitory,activity
R3736	T6304	T6299	prep	over,activity
R3737	T6305	T6304	pobj	HKI,over
R3738	T6306	T6307	punct	(,2D
R3739	T6307	T6295	parataxis	2D,has
R374	T1078	T1076	parataxis	5,trafficking
R3740	T6308	T6307	compound	Figure,2D
R3741	T6309	T6307	punct	),2D
R3742	T6310	T6281	punct	.,suppressed
R3743	T6312	T6313	det	The,effect
R3744	T6313	T6315	nsubj	effect,supports
R3745	T6314	T6313	amod	sub-stochiometry,effect
R3746	T6316	T6313	prep	of,effect
R3747	T6317	T6318	det	the,LD
R3748	T6318	T6316	pobj	LD,of
R3749	T6319	T6318	prep	of,LD
R375	T1079	T1078	nummod	3,5
R3750	T6320	T6319	pobj	RanBP2,of
R3751	T6321	T6313	prep	over,effect
R3752	T6322	T6323	det	the,inhibition
R3753	T6323	T6321	pobj	inhibition,over
R3754	T6324	T6323	prep	of,inhibition
R3755	T6325	T6324	pobj	HKI,of
R3756	T6326	T6323	prep	by,inhibition
R3757	T6327	T6326	pobj	Cox11,by
R3758	T6328	T6329	mark	that,underlies
R3759	T6329	T6315	advcl	underlies,supports
R376	T1080	T1078	punct	",",5
R3760	T6330	T6331	det	a,mechanism
R3761	T6331	T6329	nsubj	mechanism,underlies
R3762	T6332	T6333	npadvmod	LD,dependent
R3763	T6333	T6331	amod	dependent,mechanism
R3764	T6334	T6333	punct	-,dependent
R3765	T6335	T6336	npadvmod	chaperonin,like
R3766	T6336	T6331	amod	like,mechanism
R3767	T6337	T6336	punct	-,like
R3768	T6338	T6339	det	the,suppression
R3769	T6339	T6329	dobj	suppression,underlies
R377	T1081	T1078	punct	],5
R3770	T6340	T6339	prep	of,suppression
R3771	T6341	T6342	amod	Cox11,dependent
R3772	T6342	T6344	amod	dependent,inhibition
R3773	T6343	T6342	punct	-,dependent
R3774	T6344	T6340	pobj	inhibition,of
R3775	T6345	T6344	prep	of,inhibition
R3776	T6346	T6345	pobj	HKI,of
R3777	T6347	T6344	prep	by,inhibition
R3778	T6348	T6347	pobj	RanBP2,by
R3779	T6349	T6329	punct	", ",underlies
R378	T1082	T1076	punct	", ",trafficking
R3780	T6350	T6329	cc	and,underlies
R3781	T6351	T6352	mark	that,acts
R3782	T6352	T6329	conj	acts,underlies
R3783	T6353	T6352	nsubj	RanBP2,acts
R3784	T6354	T6352	prep	as,acts
R3785	T6355	T6356	det	a,buffer
R3786	T6356	T6354	pobj	buffer,as
R3787	T6357	T6356	amod	molecular,buffer
R3788	T6358	T6356	punct	“,buffer
R3789	T6359	T6356	punct	”,buffer
R379	T1083	T1084	compound	protein,biogenesis
R3790	T6360	T6356	prep	over,buffer
R3791	T6361	T6362	nmod	HK1,activities
R3792	T6362	T6360	pobj	activities,over
R3793	T6363	T6361	cc	and,HK1
R3794	T6364	T6361	conj	Cox11,HK1
R3795	T6365	T6315	punct	.,supports
R3796	T6367	T6368	det	The,loss
R3797	T6368	T6370	nsubj	loss,leads
R3798	T6369	T6368	amod	partial,loss
R3799	T6371	T6368	prep	of,loss
R38	T271	T268	prep	with,partners
R380	T1084	T1076	conj	biogenesis,trafficking
R3800	T6372	T6373	det	the,activity
R3801	T6373	T6371	pobj	activity,of
R3802	T6374	T6373	compound	RanBP2,activity
R3803	T6375	T6373	compound	chaperone,activity
R3804	T6376	T6368	prep	in,loss
R3805	T6377	T6378	nmod	RanBP2,mice
R3806	T6378	T6376	pobj	mice,in
R3807	T6379	T6377	punct	+,RanBP2
R3808	T6380	T6377	punct	/,RanBP2
R3809	T6381	T6377	punct	−,RanBP2
R381	T1085	T1086	punct	[,7
R3810	T6382	T6370	advmod	also,leads
R3811	T6383	T6370	prep	to,leads
R3812	T6384	T6383	pobj	deficits,to
R3813	T6385	T6384	prep	in,deficits
R3814	T6386	T6387	det	the,sequestration
R3815	T6387	T6385	pobj	sequestration,in
R3816	T6388	T6387	prep	of,sequestration
R3817	T6389	T6388	pobj	HKI,of
R3818	T6390	T6384	prep	in,deficits
R3819	T6391	T6392	det	the,compartment
R382	T1086	T1084	parataxis	7,biogenesis
R3820	T6392	T6390	pobj	compartment,in
R3821	T6393	T6392	amod	ellipsoid,compartment
R3822	T6394	T6392	prep	of,compartment
R3823	T6395	T6396	amod	photosensory,neurons
R3824	T6396	T6394	pobj	neurons,of
R3825	T6397	T6370	punct	.,leads
R3826	T6399	T6400	nsubj	This,underlies
R3827	T6401	T6400	advmod	possibly,underlies
R3828	T6402	T6403	det	the,mistargeting
R3829	T6403	T6400	dobj	mistargeting,underlies
R383	T1087	T1086	nummod	6,7
R3830	T6404	T6403	prep	of,mistargeting
R3831	T6405	T6404	pobj	HKI,of
R3832	T6406	T6403	prep	to,mistargeting
R3833	T6407	T6408	det	the,compartment
R3834	T6408	T6406	pobj	compartment,to
R3835	T6409	T6408	amod	myoid,compartment
R3836	T6410	T6408	prep	of,compartment
R3837	T6411	T6412	det	these,neurons
R3838	T6412	T6410	pobj	neurons,of
R3839	T6413	T6414	punct	(,S2
R384	T1088	T1086	punct	",",7
R3840	T6414	T6400	parataxis	S2,underlies
R3841	T6415	T6414	compound	Figure,S2
R3842	T6416	T6414	punct	),S2
R3843	T6417	T6400	punct	.,underlies
R3844	T6419	T6420	det	The,data
R3845	T6420	T6421	nsubj	data,support
R3846	T6422	T6423	mark	that,is
R3847	T6423	T6421	ccomp	is,support
R3848	T6424	T6425	det	the,outcome
R3849	T6425	T6423	nsubj	outcome,is
R385	T1089	T1086	punct	],7
R3850	T6426	T6425	amod	ultimate,outcome
R3851	T6427	T6425	amod	pathophysiological,outcome
R3852	T6428	T6425	prep	in,outcome
R3853	T6429	T6428	pobj	HKI,in
R3854	T6430	T6425	punct	", ",outcome
R3855	T6431	T6425	acl	caused,outcome
R3856	T6432	T6431	agent	by,caused
R3857	T6433	T6434	det	a,reduction
R3858	T6434	T6432	pobj	reduction,by
R3859	T6435	T6434	prep	in,reduction
R386	T1090	T1084	punct	", ",biogenesis
R3860	T6436	T6437	compound	RanBP2,levels
R3861	T6437	T6435	pobj	levels,in
R3862	T6438	T6437	cc	and,levels
R3863	T6439	T6440	poss	its,activity
R3864	T6440	T6437	conj	activity,levels
R3865	T6441	T6440	compound	chaperone,activity
R3866	T6442	T6423	punct	", ",is
R3867	T6443	T6444	det	the,degradation
R3868	T6444	T6423	attr	degradation,is
R3869	T6445	T6444	amod	selective,degradation
R387	T1091	T1092	det	the,formation
R3870	T6446	T6444	prep	of,degradation
R3871	T6447	T6446	pobj	HKI,of
R3872	T6448	T6449	mark	as,reflected
R3873	T6449	T6423	advcl	reflected,is
R3874	T6450	T6449	agent	by,reflected
R3875	T6451	T6452	det	the,levels
R3876	T6452	T6450	pobj	levels,by
R3877	T6453	T6452	amod	reduced,levels
R3878	T6454	T6452	prep	of,levels
R3879	T6455	T6454	pobj	HKI,of
R388	T1092	T1084	conj	formation,biogenesis
R3880	T6456	T6455	punct	(,HKI
R3881	T6457	T6455	cc	and,HKI
R3882	T6458	T6455	conj	ATP,HKI
R3883	T6459	T6454	punct	),of
R3884	T6460	T6454	cc	but,of
R3885	T6461	T6460	neg	not,but
R3886	T6462	T6454	conj	of,of
R3887	T6463	T6464	amod	other,components
R3888	T6464	T6462	pobj	components,of
R3889	T6465	T6464	amod	mitochondrial,components
R389	T1093	T1092	prep	of,formation
R3890	T6466	T6465	cc	and,mitochondrial
R3891	T6467	T6465	conj	NPC,mitochondrial
R3892	T6468	T6469	punct	(,Figure
R3893	T6469	T6423	parataxis	Figure,is
R3894	T6470	T6469	nummod	5,Figure
R3895	T6471	T6469	punct	),Figure
R3896	T6472	T6421	punct	.,support
R3897	T6474	T6475	prep	Through,is
R3898	T6476	T6477	det	this,process
R3899	T6477	T6474	pobj	process,Through
R39	T272	T273	amod	distinct,functions
R390	T1094	T1095	det	the,spindle
R3900	T6478	T6475	punct	", ",is
R3901	T6479	T6475	nsubj	it,is
R3902	T6480	T6475	advmod	also,is
R3903	T6481	T6475	acomp	possible,is
R3904	T6482	T6483	mark	that,cause
R3905	T6483	T6475	ccomp	cause,is
R3906	T6484	T6483	nsubj	deficits,cause
R3907	T6485	T6484	prep	in,deficits
R3908	T6486	T6485	pobj	RanBP2,in
R3909	T6487	T6488	det	a,disturbance
R391	T1095	T1093	pobj	spindle,of
R3910	T6488	T6483	dobj	disturbance,cause
R3911	T6489	T6488	prep	in,disturbance
R3912	T6490	T6491	det	the,equilibrium
R3913	T6491	T6489	pobj	equilibrium,in
R3914	T6492	T6491	prep	between,equilibrium
R3915	T6493	T6492	pobj	Cox11,between
R3916	T6494	T6493	punct	", ",Cox11
R3917	T6495	T6493	conj	HK1,Cox11
R3918	T6496	T6495	punct	", ",HK1
R3919	T6497	T6495	cc	and,HK1
R392	T1096	T1095	amod	mitotic,spindle
R3920	T6498	T6495	conj	RanBP2,HK1
R3921	T6499	T6483	prep	by,cause
R3922	T6500	T6499	pcomp	leading,by
R3923	T6501	T6500	prep	to,leading
R3924	T6502	T6503	det	an,increase
R3925	T6503	T6501	pobj	increase,to
R3926	T6504	T6503	prep	of,increase
R3927	T6505	T6506	det	the,activity
R3928	T6506	T6504	pobj	activity,of
R3929	T6507	T6506	amod	inhibitory,activity
R393	T1097	T1092	punct	", ",formation
R3930	T6508	T6506	prep	of,activity
R3931	T6509	T6508	pobj	Cox11,of
R3932	T6510	T6506	prep	over,activity
R3933	T6511	T6510	pobj	HKI,over
R3934	T6512	T6513	dep	that,promotes
R3935	T6513	T6506	relcl	promotes,activity
R3936	T6514	T6515	det	the,uncoupling
R3937	T6515	T6513	dobj	uncoupling,promotes
R3938	T6516	T6515	prep	of,uncoupling
R3939	T6517	T6518	det	the,interaction
R394	T1098	T1092	conj	assembly,formation
R3940	T6518	T6516	pobj	interaction,of
R3941	T6519	T6518	prep	of,interaction
R3942	T6520	T6519	pobj	HKI,of
R3943	T6521	T6515	prep	from,uncoupling
R3944	T6522	T6521	pobj	RanBP2,from
R3945	T6523	T6513	punct	", ",promotes
R3946	T6524	T6525	advmod	ultimately,causing
R3947	T6525	T6513	advcl	causing,promotes
R3948	T6526	T6527	compound	HKI,degradation
R3949	T6527	T6525	dobj	degradation,causing
R395	T1099	T1098	prep	of,assembly
R3950	T6528	T6475	punct	.,is
R3951	T6530	T6531	advmod	Regardless,contribute
R3952	T6532	T6531	punct	", ",contribute
R3953	T6533	T6534	amod	lower,levels
R3954	T6534	T6531	nsubj	levels,contribute
R3955	T6535	T6534	prep	of,levels
R3956	T6536	T6535	pobj	HKI,of
R3957	T6537	T6531	advmod	likely,contribute
R3958	T6538	T6531	prep	to,contribute
R3959	T6539	T6540	det	the,levels
R396	T1100	T1101	det	the,envelope
R3960	T6540	T6538	pobj	levels,to
R3961	T6541	T6540	amod	decreased,levels
R3962	T6542	T6540	prep	in,levels
R3963	T6543	T6542	pobj	ATP,in
R3964	T6544	T6540	punct	", ",levels
R3965	T6545	T6546	amod	slower,rates
R3966	T6546	T6540	conj	rates,levels
R3967	T6547	T6546	compound	growth,rates
R3968	T6548	T6546	punct	", ",rates
R3969	T6549	T6546	cc	and,rates
R397	T1101	T1099	pobj	envelope,of
R3970	T6550	T6551	amod	diminished,ability
R3971	T6551	T6546	conj	ability,rates
R3972	T6552	T6553	aux	to,metabolize
R3973	T6553	T6551	acl	metabolize,ability
R3974	T6554	T6553	dobj	glucose,metabolize
R3975	T6555	T6554	prep	of,glucose
R3976	T6556	T6557	nmod	RanBP2,mice
R3977	T6557	T6555	pobj	mice,of
R3978	T6558	T6556	punct	+,RanBP2
R3979	T6559	T6556	punct	/,RanBP2
R398	T1102	T1101	amod	nuclear,envelope
R3980	T6560	T6556	punct	−,RanBP2
R3981	T6561	T6562	punct	(,Figures
R3982	T6562	T6531	parataxis	Figures,contribute
R3983	T6563	T6562	nummod	5,Figures
R3984	T6564	T6565	punct	–,7
R3985	T6565	T6562	prep	7,Figures
R3986	T6566	T6562	punct	),Figures
R3987	T6567	T6531	punct	.,contribute
R3988	T6569	T6570	mark	As,discussed
R3989	T6570	T6571	advcl	discussed,account
R399	T1103	T1104	punct	[,8
R3990	T6572	T6570	advmod	subsequently,discussed
R3991	T6573	T6571	punct	", ",account
R3992	T6574	T6575	det	the,deficits
R3993	T6575	T6571	nsubj	deficits,account
R3994	T6576	T6575	compound	ATP,deficits
R3995	T6577	T6575	prep	in,deficits
R3996	T6578	T6579	det	the,retina
R3997	T6579	T6577	pobj	retina,in
R3998	T6580	T6571	advmod	likely,account
R3999	T6581	T6571	prep	for,account
R40	T273	T271	pobj	functions,with
R400	T1104	T1098	parataxis	8,assembly
R4000	T6582	T6583	det	the,responses
R4001	T6583	T6581	pobj	responses,for
R4002	T6584	T6583	amod	reduced,responses
R4003	T6585	T6583	amod	electrophysiological,responses
R4004	T6586	T6583	prep	of,responses
R4005	T6587	T6588	amod	retinal,neurons
R4006	T6588	T6586	pobj	neurons,of
R4007	T6589	T6590	punct	(,Figure
R4008	T6590	T6571	parataxis	Figure,account
R4009	T6591	T6590	nummod	8,Figure
R401	T1105	T1104	punct	],8
R4010	T6592	T6590	punct	),Figure
R4011	T6593	T6571	punct	.,account
R4012	T6595	T6596	amod	Various,domains
R4013	T6596	T6597	nsubjpass	domains,implicated
R4014	T6598	T6596	prep	of,domains
R4015	T6599	T6598	pobj	RanBP2,of
R4016	T6600	T6597	aux	have,implicated
R4017	T6601	T6597	auxpass	been,implicated
R4018	T6602	T6597	advmod	previously,implicated
R4019	T6603	T6597	prep	with,implicated
R402	T1106	T1098	punct	", ",assembly
R4020	T6604	T6605	det	a,role
R4021	T6605	T6603	pobj	role,with
R4022	T6606	T6605	compound	chaperone,role
R4023	T6607	T6605	prep	in,role
R4024	T6608	T6609	det	the,cell
R4025	T6609	T6607	pobj	cell,in
R4026	T6610	T6597	punct	.,implicated
R4027	T6612	T6613	nsubj	These,include
R4028	T6614	T6615	det	the,enhancement
R4029	T6615	T6613	dobj	enhancement,include
R403	T1107	T1098	cc	and,assembly
R4030	T6616	T6615	prep	of,enhancement
R4031	T6617	T6618	det	the,biogenesis
R4032	T6618	T6616	pobj	biogenesis,of
R4033	T6619	T6618	prep	of,biogenesis
R4034	T6620	T6621	amod	red,green
R4035	T6621	T6623	amod	green,opsin
R4036	T6622	T6621	punct	/,green
R4037	T6623	T6619	pobj	opsin,of
R4038	T6624	T6615	prep	by,enhancement
R4039	T6625	T6626	det	the,combination
R404	T1108	T1109	det	the,integration
R4040	T6626	T6624	pobj	combination,by
R4041	T6627	T6626	prep	of,combination
R4042	T6628	T6629	det	the,domains
R4043	T6629	T6627	pobj	domains,of
R4044	T6630	T6631	compound	RBD4,CY
R4045	T6631	T6629	compound	CY,domains
R4046	T6632	T6631	punct	-,CY
R4047	T6633	T6634	punct	[,7
R4048	T6634	T6615	parataxis	7,enhancement
R4049	T6635	T6634	nummod	6,7
R405	T1109	T1098	conj	integration,assembly
R4050	T6636	T6634	punct	",",7
R4051	T6637	T6634	punct	],7
R4052	T6638	T6615	cc	and,enhancement
R4053	T6639	T6640	det	the,stabilization
R4054	T6640	T6615	conj	stabilization,enhancement
R4055	T6641	T6640	prep	by,stabilization
R4056	T6642	T6643	det	the,domains
R4057	T6643	T6641	pobj	domains,by
R4058	T6644	T6645	npadvmod	Ran,binding
R4059	T6645	T6643	amod	binding,domains
R406	T1110	T1109	prep	of,integration
R4060	T6646	T6645	punct	-,binding
R4061	T6647	T6643	prep	of,domains
R4062	T6648	T6647	pobj	RanBP2,of
R4063	T6649	T6640	prep	of,stabilization
R4064	T6650	T6651	det	the,state
R4065	T6651	T6649	pobj	state,of
R4066	T6652	T6653	compound	guanosine,triphosphate
R4067	T6653	T6654	npadvmod	triphosphate,bound
R4068	T6654	T6651	amod	bound,state
R4069	T6655	T6654	punct	-,bound
R407	T1111	T1112	det	the,breakdown
R4070	T6656	T6651	amod	conformational,state
R4071	T6657	T6651	prep	of,state
R4072	T6658	T6657	pobj	RanGTPase,of
R4073	T6659	T6640	cc	and,stabilization
R4074	T6660	T6640	conj	interaction,stabilization
R4075	T6661	T6660	prep	of,interaction
R4076	T6662	T6661	pobj	Ran,of
R4077	T6663	T6660	prep	with,interaction
R4078	T6664	T6665	compound	importin,β
R4079	T6665	T6663	pobj	β,with
R408	T1112	T1110	pobj	breakdown,of
R4080	T6666	T6665	punct	-,β
R4081	T6667	T6668	punct	[,41
R4082	T6668	T6613	parataxis	41,include
R4083	T6669	T6668	nummod	11,41
R4084	T6670	T6668	punct	",",41
R4085	T6671	T6668	nummod	40,41
R4086	T6672	T6668	punct	",",41
R4087	T6673	T6668	punct	],41
R4088	T6674	T6613	punct	.,include
R4089	T6676	T6677	det	The,data
R409	T1113	T1114	amod	nuclear,envelope
R4090	T6677	T6678	nsubj	data,show
R4091	T6679	T6677	advmod	herein,data
R4092	T6680	T6681	mark	that,extends
R4093	T6681	T6678	ccomp	extends,show
R4094	T6682	T6683	det	the,role
R4095	T6683	T6681	nsubj	role,extends
R4096	T6684	T6683	amod	multi-chaperone,role
R4097	T6685	T6683	prep	of,role
R4098	T6686	T6685	pobj	RanBP2,of
R4099	T6687	T6681	advmod	also,extends
R41	T274	T273	acl	interacting,functions
R410	T1114	T1112	compound	envelope,breakdown
R4100	T6688	T6681	prep	to,extends
R4101	T6689	T6690	poss	its,LD
R4102	T6690	T6688	pobj	LD,to
R4103	T6691	T6681	prep	in,extends
R4104	T6692	T6691	pobj	light,in
R4105	T6693	T6692	prep	of,light
R4106	T6694	T6695	poss	its,ability
R4107	T6695	T6693	pobj	ability,of
R4108	T6696	T6697	aux	to,associate
R4109	T6697	T6695	acl	associate,ability
R411	T1115	T1109	prep	with,integration
R4110	T6698	T6697	prep	to,associate
R4111	T6699	T6700	amod	distinct,species
R4112	T6700	T6698	pobj	species,to
R4113	T6701	T6700	compound	folding,species
R4114	T6702	T6700	prep	of,species
R4115	T6703	T6702	pobj	Cox11,of
R4116	T6704	T6697	cc	and,associate
R4117	T6705	T6706	aux	to,prevent
R4118	T6706	T6697	conj	prevent,associate
R4119	T6707	T6706	dobj	HKI,prevent
R412	T1116	T1117	compound	kinetochore,formation
R4120	T6708	T6706	prep	from,prevent
R4121	T6709	T6710	auxpass	being,degraded
R4122	T6710	T6708	pcomp	degraded,from
R4123	T6711	T6678	punct	.,show
R4124	T6713	T6714	det	This,function
R4125	T6714	T6716	nsubj	function,is
R4126	T6715	T6714	compound	chaperone,function
R4127	T6717	T6716	acomp	likely,is
R4128	T6718	T6719	aux	to,complemented
R4129	T6719	T6717	xcomp	complemented,likely
R413	T1117	T1115	pobj	formation,with
R4130	T6720	T6719	auxpass	be,complemented
R4131	T6721	T6719	agent	by,complemented
R4132	T6722	T6723	amod	other,partners
R4133	T6723	T6721	pobj	partners,by
R4134	T6724	T6723	prep	of,partners
R4135	T6725	T6724	pobj	RanBP2,of
R4136	T6726	T6723	prep	with,partners
R4137	T6727	T6728	amod	similar,functions
R4138	T6728	T6726	pobj	functions,with
R4139	T6729	T6727	cc	and,similar
R414	T1118	T1117	cc	and,formation
R4140	T6730	T6727	conj	pleiotropic,similar
R4141	T6731	T6716	punct	.,is
R4142	T6733	T6734	prep	For,contribute
R4143	T6735	T6733	pobj	example,For
R4144	T6736	T6734	punct	", ",contribute
R4145	T6737	T6738	det	the,combination
R4146	T6738	T6734	nsubj	combination,contribute
R4147	T6739	T6738	prep	of,combination
R4148	T6740	T6741	det	the,CLD
R4149	T6741	T6739	pobj	CLD,of
R415	T1119	T1117	conj	maturation,formation
R4150	T6742	T6741	prep	of,CLD
R4151	T6743	T6742	pobj	RanBP2,of
R4152	T6744	T6738	prep	with,combination
R4153	T6745	T6746	amod	several,subunits
R4154	T6746	T6744	pobj	subunits,with
R4155	T6747	T6746	prep	of,subunits
R4156	T6748	T6749	det	the,cap
R4157	T6749	T6747	pobj	cap,of
R4158	T6750	T6749	compound	19S,cap
R4159	T6751	T6749	prep	of,cap
R416	T1120	T1109	prep	during,integration
R4160	T6752	T6753	det	the,proteasome
R4161	T6753	T6751	pobj	proteasome,of
R4162	T6754	T6755	punct	[,14
R4163	T6755	T6746	parataxis	14,subunits
R4164	T6756	T6755	punct	],14
R4165	T6757	T6738	punct	", ",combination
R4166	T6758	T6738	cc	and,combination
R4167	T6759	T6760	prep	of,with
R4168	T6760	T6738	conj	with,combination
R4169	T6761	T6762	poss	its,repeat
R417	T1121	T1122	amod	early,progression
R4170	T6762	T6759	pobj	repeat,of
R4171	T6763	T6762	amod	neighboring,repeat
R4172	T6764	T6762	amod	internal,repeat
R4173	T6765	T6762	punct	", ",repeat
R4174	T6766	T6767	compound	W1W2,IR
R4175	T6767	T6762	appos	IR,repeat
R4176	T6768	T6767	punct	/,IR
R4177	T6769	T6770	det	the,ligase
R4178	T6770	T6760	pobj	ligase,with
R4179	T6771	T6772	compound	E3,ubiquitin
R418	T1122	T1120	pobj	progression,during
R4180	T6772	T6770	compound	ubiquitin,ligase
R4181	T6773	T6772	punct	-,ubiquitin
R4182	T6774	T6770	punct	", ",ligase
R4183	T6775	T6770	appos	parkin,ligase
R4184	T6776	T6777	punct	[,20
R4185	T6777	T6775	parataxis	20,parkin
R4186	T6778	T6777	punct	],20
R4187	T6779	T6770	punct	", ",ligase
R4188	T6780	T6770	cc	and,ligase
R4189	T6781	T6782	det	the,protein
R419	T1123	T1122	amod	mitotic,progression
R4190	T6782	T6770	conj	protein,ligase
R4191	T6783	T6782	nmod	E2,protein
R4192	T6784	T6785	npadvmod	SUMO,conjugating
R4193	T6785	T6782	amod	conjugating,protein
R4194	T6786	T6784	punct	-,SUMO
R4195	T6787	T6784	nummod	1,SUMO
R4196	T6788	T6785	punct	-,conjugating
R4197	T6789	T6782	punct	", ",protein
R4198	T6790	T6782	appos	Ubc9,protein
R4199	T6791	T6792	punct	[,15
R42	T275	T274	advmod	specifically,interacting
R420	T1124	T1125	punct	[,9
R4200	T6792	T6790	parataxis	15,Ubc9
R4201	T6793	T6792	punct	],15
R4202	T6794	T6734	aux	may,contribute
R4203	T6795	T6734	prep	to,contribute
R4204	T6796	T6797	det	the,regulation
R4205	T6797	T6795	pobj	regulation,to
R4206	T6798	T6797	amod	down,regulation
R4207	T6799	T6797	punct	-,regulation
R4208	T6800	T6797	prep	of,regulation
R4209	T6801	T6800	pobj	HKI,of
R421	T1125	T1109	parataxis	9,integration
R4210	T6802	T6797	prep	by,regulation
R4211	T6803	T6804	compound	26S,proteasome
R4212	T6804	T6805	npadvmod	proteasome,mediated
R4213	T6805	T6807	amod	mediated,proteolysis
R4214	T6806	T6805	punct	-,mediated
R4215	T6807	T6802	pobj	proteolysis,by
R4216	T6808	T6807	cc	and,proteolysis
R4217	T6809	T6807	conj	modulation,proteolysis
R4218	T6810	T6807	prep	of,proteolysis
R4219	T6811	T6812	det	the,partitioning
R422	T1126	T1125	punct	],9
R4220	T6812	T6810	pobj	partitioning,of
R4221	T6813	T6812	amod	molecular,partitioning
R4222	T6814	T6813	cc	and,molecular
R4223	T6815	T6813	conj	subcellular,molecular
R4224	T6816	T6812	prep	of,partitioning
R4225	T6817	T6818	det	these,partners
R4226	T6818	T6816	pobj	partners,of
R4227	T6819	T6734	punct	.,contribute
R4228	T6821	T6822	prep	In,be
R4229	T6823	T6824	det	this,regard
R423	T1127	T1067	punct	.,emerged
R4230	T6824	T6821	pobj	regard,In
R4231	T6825	T6822	punct	", ",be
R4232	T6826	T6822	nsubj	it,be
R4233	T6827	T6822	aux	will,be
R4234	T6828	T6822	acomp	interesting,be
R4235	T6829	T6830	aux	to,probe
R4236	T6830	T6822	xcomp	probe,be
R4237	T6831	T6832	mark	whether,causes
R4238	T6832	T6830	ccomp	causes,probe
R4239	T6833	T6832	nsubj	parkin,causes
R424	T1129	T1130	det	The,interaction
R4240	T6834	T6832	advmod	also,causes
R4241	T6835	T6832	dobj	deficits,causes
R4242	T6836	T6835	prep	in,deficits
R4243	T6837	T6836	pobj	HKI,in
R4244	T6838	T6832	prep	in,causes
R4245	T6839	T6840	amod	dopaminergic,neurons
R4246	T6840	T6838	pobj	neurons,in
R4247	T6841	T6822	punct	", ",be
R4248	T6842	T6843	mark	since,reported
R4249	T6843	T6822	advcl	reported,be
R425	T1130	T1132	nsubj	interaction,reflects
R4250	T6844	T6843	nsubjpass	parkin,reported
R4251	T6845	T6843	auxpass	was,reported
R4252	T6846	T6847	aux	to,modulate
R4253	T6847	T6843	xcomp	modulate,reported
R4254	T6848	T6849	compound	RanBP2,turnover
R4255	T6849	T6847	dobj	turnover,modulate
R4256	T6850	T6851	punct	[,20
R4257	T6851	T6843	parataxis	20,reported
R4258	T6852	T6851	punct	],20
R4259	T6853	T6843	punct	", ",reported
R426	T1131	T1130	amod	specific,interaction
R4260	T6854	T6843	cc	and,reported
R4261	T6855	T6856	compound	parkin,loss
R4262	T6856	T6857	nsubj	loss,causes
R4263	T6857	T6843	conj	causes,reported
R4264	T6858	T6856	punct	-,loss
R4265	T6859	T6856	prep	of,loss
R4266	T6860	T6859	punct	-,of
R4267	T6861	T6859	pobj	function,of
R4268	T6862	T6857	advmod	also,causes
R4269	T6863	T6864	nmod	energy,deficits
R427	T1133	T1130	prep	of,interaction
R4270	T6864	T6857	dobj	deficits,causes
R4271	T6865	T6863	cc	and,energy
R4272	T6866	T6863	conj	growth,energy
R4273	T6867	T6868	punct	[,42
R4274	T6868	T6857	parataxis	42,causes
R4275	T6869	T6868	dep	26,42
R4276	T6870	T6871	punct	–,29
R4277	T6871	T6869	prep	29,26
R4278	T6872	T6868	punct	",",42
R4279	T6873	T6868	punct	],42
R428	T1134	T1133	pobj	RanBP2,of
R4280	T6874	T6822	punct	.,be
R4281	T6876	T6877	det	These,data
R4282	T6877	T6878	nsubj	data,add
R4283	T6879	T6880	det	a,dimension
R4284	T6880	T6878	dobj	dimension,add
R4285	T6881	T6880	amod	new,dimension
R4286	T6882	T6878	prep	to,add
R4287	T6883	T6884	det	the,complexity
R4288	T6884	T6882	pobj	complexity,to
R4289	T6885	T6884	prep	of,complexity
R429	T1135	T1130	prep	with,interaction
R4290	T6886	T6887	det	the,regulation
R4291	T6887	T6885	pobj	regulation,of
R4292	T6888	T6887	prep	of,regulation
R4293	T6889	T6890	det	the,pathway
R4294	T6890	T6888	pobj	pathway,of
R4295	T6891	T6890	amod	glycolytic,pathway
R4296	T6892	T6887	punct	", ",regulation
R4297	T6893	T6894	prep	in,in
R4298	T6894	T6887	prep	in,regulation
R4299	T6895	T6893	amod	particular,in
R43	T276	T274	prep	with,interacting
R430	T1136	T1137	det	a,set
R4300	T6896	T6897	det	the,CNS
R4301	T6897	T6894	pobj	CNS,in
R4302	T6898	T6897	punct	", ",CNS
R4303	T6899	T6900	advmod	where,plays
R4304	T6900	T6897	advcl	plays,CNS
R4305	T6901	T6900	nsubj	glycolysis,plays
R4306	T6902	T6903	det	a,role
R4307	T6903	T6900	dobj	role,plays
R4308	T6904	T6903	amod	major,role
R4309	T6905	T6900	prep	in,plays
R431	T1137	T1135	pobj	set,with
R4310	T6906	T6905	pcomp	supplying,in
R4311	T6907	T6906	dobj	energy,supplying
R4312	T6908	T6900	cc	and,plays
R4313	T6909	T6910	advmod	where,play
R4314	T6910	T6900	conj	play,plays
R4315	T6911	T6912	det	the,machinery
R4316	T6912	T6910	nsubj	machinery,play
R4317	T6913	T6912	compound	proteasome,machinery
R4318	T6914	T6910	aux	may,play
R4319	T6915	T6916	det	a,role
R432	T1138	T1137	amod	diverse,set
R4320	T6916	T6910	dobj	role,play
R4321	T6917	T6916	amod	critical,role
R4322	T6918	T6910	prep	in,play
R4323	T6919	T6918	pcomp	modulating,in
R4324	T6920	T6919	dobj	components,modulating
R4325	T6921	T6920	prep	of,components
R4326	T6922	T6923	det	the,machinery
R4327	T6923	T6921	pobj	machinery,of
R4328	T6924	T6925	compound	energy,supply
R4329	T6925	T6923	compound	supply,machinery
R433	T1139	T1137	prep	of,set
R4330	T6926	T6927	punct	[,43
R4331	T6927	T6910	parataxis	43,play
R4332	T6928	T6927	punct	],43
R4333	T6929	T6878	punct	.,add
R4334	T6931	T6932	nsubjpass	This,evidenced
R4335	T6933	T6932	auxpass	is,evidenced
R4336	T6934	T6932	advmod	further,evidenced
R4337	T6935	T6932	agent	by,evidenced
R4338	T6936	T6937	det	the,presence
R4339	T6937	T6935	pobj	presence,by
R434	T1140	T1139	pobj	partners,of
R4340	T6938	T6937	prep	of,presence
R4341	T6939	T6940	amod	genetic,modifiers
R4342	T6940	T6938	pobj	modifiers,of
R4343	T6941	T6937	prep	in,presence
R4344	T6942	T6943	nmod	RanBP2,mice
R4345	T6943	T6941	pobj	mice,in
R4346	T6944	T6942	punct	+,RanBP2
R4347	T6945	T6942	punct	/,RanBP2
R4348	T6946	T6942	punct	−,RanBP2
R4349	T6947	T6943	prep	on,mice
R435	T1141	T1132	advmod	likely,reflects
R4350	T6948	T6949	det	a,background
R4351	T6949	T6947	pobj	background,on
R4352	T6950	T6949	amod	mixed,background
R4353	T6951	T6949	amod	genetic,background
R4354	T6952	T6953	dep	that,compensates
R4355	T6953	T6949	relcl	compensates,background
R4356	T6954	T6953	prep	for,compensates
R4357	T6955	T6954	pobj	deficits,for
R4358	T6956	T6955	prep	in,deficits
R4359	T6957	T6956	pobj	RanBP2,in
R436	T1142	T1143	det	a,role
R4360	T6958	T6955	cc	and,deficits
R4361	T6959	T6955	conj	deregulation,deficits
R4362	T6960	T6959	prep	of,deregulation
R4363	T6961	T6962	poss	its,partners
R4364	T6962	T6960	pobj	partners,of
R4365	T6963	T6962	prep	in,partners
R4366	T6964	T6965	compound	glucose,energy
R4367	T6965	T6967	compound	energy,homeostasis
R4368	T6966	T6965	punct	/,energy
R4369	T6967	T6963	pobj	homeostasis,in
R437	T1143	T1132	dobj	role,reflects
R4370	T6968	T6969	mark	as,observed
R4371	T6969	T6953	advcl	observed,compensates
R4372	T6970	T6969	prep	in,observed
R4373	T6971	T6972	det	the,line
R4374	T6972	T6970	pobj	line,in
R4375	T6973	T6972	amod	coisogenic,line
R4376	T6974	T6932	punct	.,evidenced
R4377	T6976	T6977	det	The,presence
R4378	T6977	T6978	nsubjpass	presence,supported
R4379	T6979	T6977	prep	of,presence
R438	T1144	T1143	amod	pleiotropic,role
R4380	T6980	T6981	amod	such,mechanisms
R4381	T6981	T6979	pobj	mechanisms,of
R4382	T6982	T6981	amod	compensatory,mechanisms
R4383	T6983	T6978	auxpass	is,supported
R4384	T6984	T6978	advmod	also,supported
R4385	T6985	T6978	agent	by,supported
R4386	T6986	T6987	det	the,identification
R4387	T6987	T6985	pobj	identification,by
R4388	T6988	T6987	prep	of,identification
R4389	T6989	T6990	det	a,locus
R439	T1145	T1143	prep	of,role
R4390	T6990	T6988	pobj	locus,of
R4391	T6991	T6990	amod	quantitative,locus
R4392	T6992	T6990	compound	trait,locus
R4393	T6993	T6990	punct	", ",locus
R4394	T6994	T6995	dep	which,encompasses
R4395	T6995	T6990	relcl	encompasses,locus
R4396	T6996	T6995	dobj	RanBP2,encompasses
R4397	T6997	T6978	punct	", ",supported
R4398	T6998	T6978	cc	and,supported
R4399	T6999	T6978	conj	modifies,supported
R44	T277	T278	amod	selective,modules
R440	T1146	T1145	pobj	RanBP2,of
R4400	T7000	T7001	det	the,expression
R4401	T7001	T6999	dobj	expression,modifies
R4402	T7002	T7001	prep	of,expression
R4403	T7003	T7004	npadvmod	diabetes,related
R4404	T7004	T7006	amod	related,phenotypes
R4405	T7005	T7004	punct	-,related
R4406	T7006	T7002	pobj	phenotypes,of
R4407	T7007	T7008	punct	[,44
R4408	T7008	T6999	parataxis	44,modifies
R4409	T7009	T7008	punct	],44
R441	T1147	T1143	prep	in,role
R4410	T7010	T6978	punct	.,supported
R4411	T7012	T7013	det	The,data
R4412	T7013	T7014	nsubj	data,show
R4413	T7015	T7013	advmod	herein,data
R4414	T7016	T7014	dobj	tissues,show
R4415	T7017	T7016	prep	with,tissues
R4416	T7018	T7019	det	a,decrease
R4417	T7019	T7017	pobj	decrease,with
R4418	T7020	T7019	prep	of,decrease
R4419	T7021	T7020	pobj	RanBP2,of
R442	T1148	T1149	compound	cell,function
R4420	T7022	T7014	cc	and,show
R4421	T7023	T7024	compound	HKI,levels
R4422	T7024	T7025	nsubj	levels,mirror
R4423	T7025	T7014	conj	mirror,show
R4424	T7026	T7027	det	a,reduction
R4425	T7027	T7025	dobj	reduction,mirror
R4426	T7028	T7027	prep	in,reduction
R4427	T7029	T7030	det	the,levels
R4428	T7030	T7028	pobj	levels,in
R4429	T7031	T7030	compound	ATP,levels
R443	T1149	T1147	pobj	function,in
R4430	T7032	T7025	punct	.,mirror
R4431	T7034	T7035	mark	Since,consumes
R4432	T7035	T7065	advcl	consumes,underlies
R4433	T7036	T7037	det	the,pump
R4434	T7037	T7035	nsubj	pump,consumes
R4435	T7038	T7037	amod	constitutive,pump
R4436	T7039	T7040	compound	Na+,K+
R4437	T7040	T7037	compound	K+,pump
R4438	T7041	T7040	punct	/,K+
R4439	T7042	T7037	compound	ATPase,pump
R444	T1150	T1143	punct	", ",role
R4440	T7043	T7037	cc	and,pump
R4441	T7044	T7045	det	the,conversion
R4442	T7045	T7037	conj	conversion,pump
R4443	T7046	T7047	npadvmod	ATP,dependent
R4444	T7047	T7045	amod	dependent,conversion
R4445	T7048	T7047	punct	-,dependent
R4446	T7049	T7045	prep	of,conversion
R4447	T7050	T7049	pobj	glutamine,of
R4448	T7051	T7045	prep	to,conversion
R4449	T7052	T7051	pobj	glutamate,to
R445	T1151	T1152	advmod	possibly,through
R4450	T7053	T7035	punct	(,consumes
R4451	T7054	T7055	nsubj	both,fundamental
R4452	T7055	T7035	advcl	fundamental,consumes
R4453	T7056	T7057	aux	to,maintain
R4454	T7057	T7055	advcl	maintain,fundamental
R4455	T7058	T7059	det	the,activity
R4456	T7059	T7057	dobj	activity,maintain
R4457	T7060	T7059	amod	electrical,activity
R4458	T7061	T7057	prep	in,maintain
R4459	T7062	T7063	det	the,CNS
R446	T1152	T1143	prep	through,role
R4460	T7063	T7061	pobj	CNS,in
R4461	T7064	T7035	punct	),consumes
R4462	T7066	T7067	det	the,majority
R4463	T7067	T7035	dobj	majority,consumes
R4464	T7068	T7067	amod	vast,majority
R4465	T7069	T7067	prep	of,majority
R4466	T7070	T7071	det	the,energy
R4467	T7071	T7069	pobj	energy,of
R4468	T7072	T7071	acl	produced,energy
R4469	T7073	T7072	agent	by,produced
R447	T1153	T1154	det	the,integration
R4470	T7074	T7075	det	the,CNS
R4471	T7075	T7073	pobj	CNS,by
R4472	T7076	T7077	punct	[,45
R4473	T7077	T7035	parataxis	45,consumes
R4474	T7078	T7079	punct	–,47
R4475	T7079	T7077	prep	47,45
R4476	T7080	T7077	punct	],45
R4477	T7081	T7065	punct	", ",underlies
R4478	T7082	T7065	nsubj	this,underlies
R4479	T7083	T7065	advmod	likely,underlies
R448	T1154	T1152	pobj	integration,through
R4480	T7084	T7085	det	the,suppression
R4481	T7085	T7065	dobj	suppression,underlies
R4482	T7086	T7085	prep	of,suppression
R4483	T7087	T7088	det	the,responses
R4484	T7088	T7086	pobj	responses,of
R4485	T7089	T7090	amod	electrophysiological,output
R4486	T7090	T7088	compound	output,responses
R4487	T7091	T7088	prep	of,responses
R4488	T7092	T7093	amod	retinal,neurons
R4489	T7093	T7091	pobj	neurons,of
R449	T1155	T1154	prep	of,integration
R4490	T7094	T7095	punct	(,Figure
R4491	T7095	T7065	parataxis	Figure,underlies
R4492	T7096	T7095	nummod	8,Figure
R4493	T7097	T7095	punct	),Figure
R4494	T7098	T7065	punct	.,underlies
R4495	T7100	T7101	advmod	Moreover,lead
R4496	T7102	T7101	punct	", ",lead
R4497	T7103	T7101	nsubj	deficits,lead
R4498	T7104	T7103	prep	in,deficits
R4499	T7105	T7104	pobj	HKI,in
R45	T278	T276	pobj	modules,with
R450	T1156	T1157	amod	multiple,pathways
R4500	T7106	T7101	aux	may,lead
R4501	T7107	T7101	prep	to,lead
R4502	T7108	T7109	amod	intracellular,hyperglycemia
R4503	T7109	T7107	pobj	hyperglycemia,to
R4504	T7110	T7101	prep	in,lead
R4505	T7111	T7112	det	the,CNS
R4506	T7112	T7110	pobj	CNS,in
R4507	T7113	T7101	punct	", ",lead
R4508	T7114	T7101	conj	promote,lead
R4509	T7115	T7116	npadvmod	sorbitol,induced
R451	T1157	T1155	pobj	pathways,of
R4510	T7116	T7118	amod	induced,stress
R4511	T7117	T7116	punct	-,induced
R4512	T7118	T7114	dobj	stress,promote
R4513	T7119	T7118	amod	osmotic,stress
R4514	T7120	T7114	punct	", ",promote
R4515	T7121	T7114	cc	and,promote
R4516	T7122	T7114	conj	compromise,promote
R4517	T7123	T7122	advmod	further,compromise
R4518	T7124	T7125	det	the,activity
R4519	T7125	T7122	dobj	activity,compromise
R452	T1158	T1132	punct	.,reflects
R4520	T7126	T7127	npadvmod	ATPase,dependent
R4521	T7127	T7125	amod	dependent,activity
R4522	T7128	T7127	punct	-,dependent
R4523	T7129	T7130	compound	Na+,K+
R4524	T7130	T7125	compound	K+,activity
R4525	T7131	T7130	punct	/,K+
R4526	T7132	T7125	compound	pump,activity
R4527	T7133	T7134	punct	[,48
R4528	T7134	T7122	parataxis	48,compromise
R4529	T7135	T7136	punct	–,50
R453	T1160	T1161	prep	On,impart
R4530	T7136	T7134	prep	50,48
R4531	T7137	T7134	punct	],48
R4532	T7138	T7101	punct	.,lead
R4533	T7140	T7141	nsubjpass	This,exacerbated
R4534	T7142	T7141	aux	may,exacerbated
R4535	T7143	T7141	auxpass	be,exacerbated
R4536	T7144	T7141	agent	by,exacerbated
R4537	T7145	T7146	det	a,decrease
R4538	T7146	T7144	pobj	decrease,by
R4539	T7147	T7146	prep	of,decrease
R454	T1162	T1163	det	the,hand
R4540	T7148	T7147	pobj	ATP,of
R4541	T7149	T7141	punct	", ",exacerbated
R4542	T7150	T7151	mark	since,stimulated
R4543	T7151	T7141	advcl	stimulated,exacerbated
R4544	T7152	T7153	det	the,activity
R4545	T7153	T7151	nsubjpass	activity,stimulated
R4546	T7154	T7153	prep	of,activity
R4547	T7155	T7154	pobj	HKI,of
R4548	T7156	T7151	auxpass	is,stimulated
R4549	T7157	T7151	advmod	also,stimulated
R455	T1163	T1160	pobj	hand,On
R4550	T7158	T7151	agent	by,stimulated
R4551	T7159	T7158	pobj	ATP,by
R4552	T7160	T7161	punct	[,51
R4553	T7161	T7151	parataxis	51,stimulated
R4554	T7162	T7161	punct	],51
R4555	T7163	T7141	punct	.,exacerbated
R4556	T7165	T7166	det	The,effects
R4557	T7166	T7168	nsubjpass	effects,known
R4558	T7167	T7166	amod	cumulative,effects
R4559	T7169	T7166	prep	of,effects
R456	T1164	T1163	amod	other,hand
R4560	T7170	T7171	det	a,reduction
R4561	T7171	T7169	pobj	reduction,of
R4562	T7172	T7171	prep	in,reduction
R4563	T7173	T7172	pobj	ATP,in
R4564	T7174	T7171	cc	and,reduction
R4565	T7175	T7176	amod	intracellular,hyperglycemia
R4566	T7176	T7171	conj	hyperglycemia,reduction
R4567	T7177	T7168	auxpass	are,known
R4568	T7178	T7179	aux	to,act
R4569	T7179	T7168	xcomp	act,known
R457	T1165	T1161	punct	", ",impart
R4570	T7180	T7179	advmod	synergistically,act
R4571	T7181	T7179	cc	and,act
R4572	T7182	T7179	conj	modulate,act
R4573	T7183	T7184	det	the,properties
R4574	T7184	T7182	dobj	properties,modulate
R4575	T7185	T7184	amod	electrophysiological,properties
R4576	T7186	T7184	prep	of,properties
R4577	T7187	T7188	amod	neuronal,activity
R4578	T7188	T7186	pobj	activity,of
R4579	T7189	T7168	punct	.,known
R458	T1166	T1167	det	the,interaction
R4580	T7191	T7192	nsubj	Figure,integrates
R4581	T7193	T7191	nummod	9,Figure
R4582	T7194	T7192	prep	in,integrates
R4583	T7195	T7196	det	a,model
R4584	T7196	T7194	pobj	model,in
R4585	T7197	T7198	det	these,variables
R4586	T7198	T7192	dobj	variables,integrates
R4587	T7199	T7198	cc	and,variables
R4588	T7200	T7198	conj	implications,variables
R4589	T7201	T7200	prep	of,implications
R459	T1167	T1161	nsubj	interaction,impart
R4590	T7202	T7203	det	the,data
R4591	T7203	T7201	pobj	data,of
R4592	T7204	T7203	acl	presented,data
R4593	T7205	T7204	advmod	herein,presented
R4594	T7206	T7192	punct	.,integrates
R4595	T7208	T7209	advmod	Still,become
R4596	T7210	T7209	punct	", ",become
R4597	T7211	T7212	amod	other,partners
R4598	T7212	T7209	nsubj	partners,become
R4599	T7213	T7214	advmod	bona,fide
R46	T279	T278	prep	of,modules
R460	T1168	T1169	npadvmod	cell,selective
R4600	T7214	T7212	amod	fide,partners
R4601	T7215	T7212	compound	RanBP2,partners
R4602	T7216	T7217	advmod	previously,identified
R4603	T7217	T7212	acl	identified,partners
R4604	T7218	T7217	cc	and,identified
R4605	T7219	T7217	conj	described,identified
R4606	T7220	T7219	prep	in,described
R4607	T7221	T7222	det	the,Introduction
R4608	T7222	T7220	pobj	Introduction,in
R4609	T7223	T7209	advmod	also,become
R461	T1169	T1167	amod	selective,interaction
R4610	T7224	T7225	amod	strong,candidates
R4611	T7225	T7209	attr	candidates,become
R4612	T7226	T7227	aux	to,play
R4613	T7227	T7225	advcl	play,candidates
R4614	T7228	T7229	det	a,role
R4615	T7229	T7227	dobj	role,play
R4616	T7230	T7227	prep	in,play
R4617	T7231	T7232	compound	energy,homeostasis
R4618	T7232	T7230	pobj	homeostasis,in
R4619	T7233	T7209	punct	.,become
R462	T1170	T1168	punct	(,cell
R4620	T7235	T7236	prep	For,modulated
R4621	T7274	T7267	punct	),degradation
R4622	T7237	T7235	pobj	example,For
R4623	T7275	T7276	punct	[,43
R4624	T7276	T7264	parataxis	43,contribute
R4625	T7238	T7236	punct	", ",modulated
R4626	T7277	T7276	punct	],43
R4627	T7278	T7236	punct	", ",modulated
R4628	T7239	T7240	det	the,activity
R4629	T7279	T7280	mark	while,mediate
R463	T1171	T1168	appos	tissue,cell
R4630	T7280	T7236	advcl	mediate,modulated
R4631	T7281	T7280	nsubj	association,mediate
R4632	T7282	T7281	prep	of,association
R4633	T7283	T7284	amod	nuclear,receptor
R4634	T7284	T7282	pobj	receptor,of
R4635	T7240	T7236	nsubjpass	activity,modulated
R4636	T7285	T7284	compound	import,receptor
R4637	T7286	T7284	punct	", ",receptor
R4638	T7287	T7288	compound	importin,β
R4639	T7241	T7242	amod	unfolding,chaperone
R464	T1172	T1169	punct	),selective
R4640	T7288	T7284	appos	β,receptor
R4641	T7289	T7288	punct	-,β
R4642	T7290	T7281	prep	with,association
R4643	T7242	T7240	compound	chaperone,activity
R4644	T7291	T7292	det	the,domains
R4645	T7292	T7290	pobj	domains,with
R4646	T7293	T7294	npadvmod	Ran,binding
R4647	T7243	T7242	cc	and,chaperone
R4648	T7294	T7292	amod	binding,domains
R4649	T7295	T7294	punct	-,binding
R465	T1173	T1169	punct	-,selective
R4650	T7244	T7240	prep	of,activity
R4651	T7296	T7292	prep	of,domains
R4652	T7297	T7296	pobj	RanBP2,of
R4653	T7298	T7299	punct	[,11
R4654	T7245	T7244	pobj	components,of
R4655	T7299	T7281	parataxis	11,association
R4656	T7300	T7299	nummod	10,11
R4657	T7301	T7299	punct	",",11
R4658	T7246	T7245	prep	of,components
R4659	T7302	T7299	punct	],11
R466	T1174	T1167	prep	of,interaction
R4660	T7303	T7280	punct	", ",mediate
R4661	T7247	T7248	det	the,cap
R4662	T7304	T7280	aux	may,mediate
R4663	T7305	T7306	det	the,translocation
R4664	T7306	T7280	dobj	translocation,mediate
R4665	T7307	T7306	amod	nuclear,translocation
R4666	T7248	T7246	pobj	cap,of
R4667	T7308	T7306	prep	of,translocation
R4668	T7309	T7310	amod	multifunctional,substrates
R4669	T7249	T7248	compound	19S,cap
R467	T1175	T1174	pobj	RanBP2,of
R4670	T7310	T7308	pobj	substrates,of
R4671	T7311	T7310	punct	", ",substrates
R4672	T7312	T7313	amod	such,as
R4673	T7250	T7248	prep	of,cap
R4674	T7313	T7310	prep	as,substrates
R4675	T7314	T7315	nmod	glyceraldehyde,phosphate
R4676	T7251	T7252	det	the,proteasome
R4677	T7315	T7319	compound	phosphate,dehydrogenase
R4678	T7252	T7250	pobj	proteasome,of
R4679	T7316	T7315	punct	-,phosphate
R468	T1176	T1167	prep	with,interaction
R4680	T7317	T7315	nummod	3,phosphate
R4681	T7318	T7315	punct	-,phosphate
R4682	T7319	T7313	pobj	dehydrogenase,as
R4683	T7320	T7236	punct	.,modulated
R4684	T7253	T7236	aux	may,modulated
R4685	T7322	T7323	det	This,process
R4686	T7323	T7325	nsubjpass	process,inhibited
R4687	T7254	T7236	auxpass	be,modulated
R4688	T7324	T7323	compound	trafficking,process
R4689	T7326	T7325	auxpass	is,inhibited
R469	T1177	T1176	pobj	some,with
R4690	T7255	T7236	agent	by,modulated
R4691	T7327	T7325	advmod	selectively,inhibited
R4692	T7328	T7325	agent	by,inhibited
R4693	T7329	T7330	det	the,drug
R4694	T7256	T7257	det	the,CLD
R4695	T7330	T7328	pobj	drug,by
R4696	T7331	T7330	amod	neuroprotective,drug
R4697	T7332	T7330	punct	", ",drug
R4698	T7257	T7255	pobj	CLD,by
R4699	T7333	T7334	nmod	R,deprenyl
R47	T280	T279	pobj	RanBP2,of
R470	T1178	T1177	prep	of,some
R4700	T7334	T7330	appos	deprenyl,drug
R4701	T7258	T7257	prep	of,CLD
R4702	T7335	T7333	punct	-,R
R4703	T7336	T7333	punct	(,R
R4704	T7337	T7333	punct	-,R
R4705	T7259	T7258	pobj	RanBP2,of
R4706	T7338	T7333	punct	),R
R4707	T7339	T7333	punct	-,R
R4708	T7260	T7261	punct	[,14
R4709	T7340	T7330	cc	and,drug
R471	T1179	T1180	poss	its,partners
R4710	T7341	T7330	conj	derivates,drug
R4711	T7342	T7341	advmod	thereof,derivates
R4712	T7261	T7236	parataxis	14,modulated
R4713	T7343	T7325	punct	", ",inhibited
R4714	T7344	T7325	cc	and,inhibited
R4715	T7262	T7261	punct	],14
R4716	T7345	T7346	auxpass	are,employed
R4717	T7346	T7325	conj	employed,inhibited
R4718	T7347	T7346	advmod	often,employed
R4719	T7263	T7236	cc	and,modulated
R472	T1180	T1178	pobj	partners,of
R4720	T7348	T7349	aux	to,treat
R4721	T7349	T7346	advcl	treat,employed
R4722	T7350	T7351	compound	Parkinson,disease
R4723	T7264	T7236	conj	contribute,modulated
R4724	T7351	T7349	dobj	disease,treat
R4725	T7352	T7353	punct	[,52
R4726	T7353	T7346	parataxis	52,employed
R4727	T7265	T7264	prep	to,contribute
R4728	T7354	T7355	punct	–,57
R4729	T7355	T7353	prep	57,52
R473	T1181	T1161	aux	may,impart
R4730	T7356	T7353	punct	],52
R4731	T7266	T7267	det	the,degradation
R4732	T7357	T7325	punct	.,inhibited
R4733	T7359	T7360	advmod	Hence,emerge
R4734	T7361	T7360	punct	", ",emerge
R4735	T7267	T7265	pobj	degradation,to
R4736	T7362	T7360	nsubj	RanBP2,emerge
R4737	T7363	T7362	cc	and,RanBP2
R4738	T7364	T7365	poss	its,partners
R4739	T7268	T7267	amod	selective,degradation
R474	T1182	T1161	advmod	also,impart
R4740	T7365	T7362	conj	partners,RanBP2
R4741	T7366	T7360	prep	as,emerge
R4742	T7367	T7368	amod	key,players
R4743	T7269	T7267	nmod	HKI,degradation
R4744	T7368	T7366	pobj	players,as
R4745	T7270	T7269	punct	(,HKI
R4746	T7271	T7269	cc	and,HKI
R4747	T7369	T7368	cc	and,players
R4748	T7370	T7371	compound	target,genes
R4749	T7371	T7368	conj	genes,players
R475	T1183	T1184	npadvmod	cell,restricted
R4750	T7272	T7273	amod	other,substrates
R4751	T7372	T7360	prep	in,emerge
R4752	T7373	T7372	pcomp	mediating,in
R4753	T7374	T7375	amod	neuropathophysiological,mechanisms
R4754	T7273	T7269	conj	substrates,HKI
R4755	T7375	T7373	dobj	mechanisms,mediating
R4756	T7376	T7375	acl	implicated,mechanisms
R4757	T7377	T7376	prep	in,implicated
R4758	T7379	T7377	pobj	lesions,in
R4759	T7378	T7379	amod	various,lesions
R476	T1184	T1186	amod	restricted,roles
R4760	T7380	T7379	amod	genetic,lesions
R4761	T7381	T7380	cc	and,genetic
R4762	T7382	T7380	conj	environmental,genetic
R4763	T7383	T7379	prep	to,lesions
R4764	T7384	T7385	det	the,CNS
R4765	T7385	T7383	pobj	CNS,to
R4766	T7455	T7456	advmod	"Figure 9 Model Depicting a Role of RanBP2 and Some of Its Partners in Metabolic and Neuronal Function RanBP2 interacts with Cox11 and HKI and the triad is in equilibrium under normal physiological conditions. RanBP2 prevents the inhibition of HKI by Cox11 and its degradation. The ultimate effect of RanBP2 on its partners is the stimulation of the glycolytic pathway and production of ATP. The glycolytic pathway is critical to fuel the constitutive Na+/K+-ATPase pump to maintain the dark current between the inner and outer segment compartments of photosensory neurons. A deficit (haploinsufficiency) in RanBP2 disturbs the equilibrium between RanBP2, HKI, and Cox11. This pathophysiological event promotes the destabilization and degradation of HKI and a decrease in ATP production required to maintain the depolarization state neurons, and, hence, a reduction in the response of receptoral and postreceptoral neurons. A reduction in ATP levels also negatively modulates HKI activity/level. Decreased levels of HKI promote intracellular hyperglycemia and activate stress kinases, which modulate negatively the Na+/K+-ATPase pump by phosphorylation. Pathophysiological pathways promoted by RanBP2 haploinsufficiency are represented by dash lines. RIS, rod inner segment; ROS, rod outer segment. Finally",appears
R4767	T7386	T7379	punct	", ",lesions
R4768	T7457	T7456	punct	", ",appears
R4769	T7458	T7456	nsubj	RanBP2,appears
R477	T1185	T1184	punct	-,restricted
R4770	T7387	T7379	prep	as,lesions
R4771	T7459	T7460	aux	to,join
R4772	T7460	T7456	xcomp	join,appears
R4773	T7388	T7387	prep	in,as
R4774	T7461	T7462	amod	other,nucleoporins
R4775	T7462	T7460	dobj	nucleoporins,join
R4776	T7463	T7462	punct	", ",nucleoporins
R4777	T7389	T7388	pobj	patients,in
R4778	T7464	T7465	amod	such,as
R4779	T7390	T7389	prep	with,patients
R478	T1186	T1161	dobj	roles,impart
R4780	T7465	T7462	prep	as,nucleoporins
R4781	T7466	T7465	pobj	Nup96,as
R4782	T7467	T7468	punct	[,58
R4783	T7391	T7390	pobj	Parkinson,with
R4784	T7392	T7391	punct	", ",Parkinson
R4785	T7393	T7391	conj	diabetes,Parkinson
R4786	T7468	T7460	parataxis	58,join
R4787	T7469	T7468	punct	],58
R4788	T7470	T7460	punct	", ",join
R4789	T7394	T7393	prep	with,diabetes
R479	T1187	T1161	prep	to,impart
R4790	T7471	T7460	prep	in,join
R4791	T7472	T7473	amod	novel,functions
R4792	T7473	T7471	pobj	functions,in
R4793	T7395	T7396	compound	insulin,resistance
R4794	T7474	T7473	amod	physiological,functions
R4795	T7475	T7476	dep	that,are
R4796	T7476	T7473	advcl	are,functions
R4797	T7396	T7394	pobj	resistance,with
R4798	T7477	T7476	acomp	vital,are
R4799	T7478	T7477	prep	for,vital
R48	T281	T270	aux	have,identified
R480	T1188	T1187	pobj	RanBP2,to
R4800	T7397	T7396	punct	-,resistance
R4801	T7479	T7480	amod	complex,organisms
R4802	T7480	T7478	pobj	organisms,for
R4803	T7481	T7476	cc	and,are
R4804	T7398	T7393	punct	", ",diabetes
R4805	T7482	T7483	dep	that,anticipated
R4806	T7483	T7476	conj	anticipated,are
R4807	T7484	T7483	auxpass	were,anticipated
R4808	T7399	T7393	cc	and,diabetes
R4809	T7485	T7483	neg	not,anticipated
R481	T1189	T1161	punct	.,impart
R4810	T7486	T7483	prep	from,anticipated
R4811	T7487	T7488	npadvmod	cell,based
R4812	T7400	T7401	amod	other,neuropathies
R4813	T7488	T7490	amod	based,studies
R4814	T7489	T7488	punct	-,based
R4815	T7490	T7486	pobj	studies,from
R4816	T7401	T7393	conj	neuropathies,diabetes
R4817	T7491	T7456	punct	.,appears
R4818	T7402	T7401	cc	and,neuropathies
R4819	T7493	T7494	det	The,expression
R482	T1191	T1192	prep	For,associate
R4820	T7494	T7496	nsubj	expression,generates
R4821	T7495	T7494	amod	reduced,expression
R4822	T7403	T7404	amod	neurodegenerative,diseases
R4823	T7497	T7494	prep	of,expression
R4824	T7404	T7401	conj	diseases,neuropathies
R4825	T7498	T7499	det	these,proteins
R4826	T7499	T7497	pobj	proteins,of
R4827	T7405	T7401	punct	", ",neuropathies
R4828	T7500	T7494	prep	in,expression
R4829	T7501	T7502	amod	whole,animal
R483	T1193	T1191	pobj	example,For
R4830	T7502	T7504	compound	animal,models
R4831	T7406	T7407	advmod	often,linked
R4832	T7503	T7502	punct	-,animal
R4833	T7504	T7500	pobj	models,in
R4834	T7407	T7401	acl	linked,neuropathies
R4835	T7505	T7496	dobj	phenotypes,generates
R4836	T7506	T7507	dep	that,are
R4837	T7507	T7505	relcl	are,phenotypes
R4838	T7508	T7509	npadvmod	tissue,restricted
R4839	T7509	T7507	acomp	restricted,are
R484	T1194	T1192	punct	", ",associate
R4840	T7510	T7509	punct	-,restricted
R4841	T7408	T7407	prep	to,linked
R4842	T7511	T7507	cc	and,are
R4843	T7512	T7513	advmod	more,importantly
R4844	T7513	T7514	advmod	importantly,seem
R4845	T7514	T7507	conj	seem,are
R4846	T7409	T7410	compound	aging,manifestations
R4847	T7515	T7514	punct	", ",seem
R4848	T7516	T7514	aux	do,seem
R4849	T7517	T7514	neg	not,seem
R485	T1195	T1196	det	the,domains
R4850	T7410	T7408	pobj	manifestations,to
R4851	T7518	T7519	aux	to,recapitulate
R4852	T7519	T7514	xcomp	recapitulate,seem
R4853	T7411	T7360	punct	.,emerge
R4854	T7520	T7519	dobj	phenotypes,recapitulate
R4855	T7521	T7520	acl	observed,phenotypes
R4856	T7522	T7521	prep	with,observed
R4857	T7523	T7524	compound	knockdown,experiments
R4858	T7413	T7414	prep	To,serve
R4859	T7524	T7522	pobj	experiments,with
R486	T1196	T1192	nsubj	domains,associate
R4860	T7525	T7524	prep	in,experiments
R4861	T7526	T7527	npadvmod	cell,based
R4862	T7415	T7416	det	this,effect
R4863	T7416	T7413	pobj	effect,To
R4864	T7527	T7529	amod	based,assays
R4865	T7528	T7527	punct	-,based
R4866	T7529	T7525	pobj	assays,in
R4867	T7417	T7414	punct	", ",serve
R4868	T7530	T7529	compound	culture,assays
R4869	T7531	T7496	punct	.,generates
R487	T1197	T1198	npadvmod	Ran,binding
R4870	T7418	T7419	det	the,model
R4871	T7533	T7534	prep	For,shown
R4872	T7419	T7414	nsubj	model,serve
R4873	T7535	T7533	pobj	example,For
R4874	T7536	T7534	punct	", ",shown
R4875	T7537	T7538	amod	short,knockdown
R4876	T7420	T7419	compound	RanBP2,model
R4877	T7421	T7419	compound	mouse,model
R4878	T7538	T7534	nsubj	knockdown,shown
R4879	T7422	T7414	aux	will,serve
R488	T1198	T1196	amod	binding,domains
R4880	T7539	T7538	amod	interfering,knockdown
R4881	T7540	T7541	npadvmod	RNA,mediated
R4882	T7423	T7414	prep	as,serve
R4883	T7541	T7538	amod	mediated,knockdown
R4884	T7542	T7541	punct	-,mediated
R4885	T7543	T7538	prep	of,knockdown
R4886	T7424	T7425	det	a,tool
R4887	T7544	T7543	pobj	RanBP2,of
R4888	T7545	T7546	punct	[,9
R4889	T7546	T7538	parataxis	9,knockdown
R489	T1199	T1198	punct	-,binding
R4890	T7547	T7546	punct	],9
R4891	T7548	T7538	cc	and,knockdown
R4892	T7425	T7423	pobj	tool,as
R4893	T7549	T7538	conj	members,knockdown
R4894	T7550	T7549	prep	of,members
R4895	T7551	T7552	det	the,complex
R4896	T7426	T7425	amod	unique,tool
R4897	T7552	T7550	pobj	complex,of
R4898	T7553	T7554	compound	Nup107,Nup160
R4899	T7554	T7552	compound	Nup160,complex
R49	T282	T270	auxpass	been,identified
R490	T1200	T1201	nsubj	RBDn,1
R4900	T7427	T7425	amod	genetic,tool
R4901	T7555	T7554	punct	-,Nup160
R4902	T7556	T7557	punct	[,59
R4903	T7557	T7549	parataxis	59,members
R4904	T7428	T7429	aux	to,probe
R4905	T7558	T7559	punct	–,61
R4906	T7559	T7557	prep	61,59
R4907	T7560	T7557	punct	],59
R4908	T7429	T7425	advcl	probe,tool
R4909	T7561	T7534	aux	have,shown
R491	T1201	T1196	appos	1,domains
R4910	T7562	T7534	punct	", ",shown
R4911	T7563	T7534	advmod	respectively,shown
R4912	T7430	T7431	amod	selective,pathways
R4913	T7564	T7534	punct	", ",shown
R4914	T7565	T7566	aux	to,cause
R4915	T7566	T7534	xcomp	cause,shown
R4916	T7431	T7429	dobj	pathways,probe
R4917	T7567	T7568	amod	mitotic,arrest
R4918	T7568	T7566	dobj	arrest,cause
R4919	T7432	T7430	punct	", ",selective
R492	T1202	T1201	punct	=,1
R4920	T7569	T7568	cc	and,arrest
R4921	T7570	T7568	conj	disruption,arrest
R4922	T7571	T7570	prep	of,disruption
R4923	T7433	T7430	conj	multiple,selective
R4924	T7572	T7573	amod	nuclear,pore
R4925	T7573	T7574	compound	pore,assembly
R4926	T7434	T7433	cc	and,multiple
R4927	T7435	T7433	conj	novel,multiple
R4928	T7436	T7431	punct	", ",pathways
R4929	T7574	T7571	pobj	assembly,of
R493	T1203	T1204	punct	-,4
R4930	T7575	T7570	cc	and,disruption
R4931	T7437	T7438	dep	which,anticipated
R4932	T7576	T7570	conj	deficits,disruption
R4933	T7577	T7576	prep	in,deficits
R4934	T7438	T7431	relcl	anticipated,pathways
R4935	T7578	T7579	compound	mRNA,export
R4936	T7579	T7577	pobj	export,in
R4937	T7580	T7566	prep	in,cause
R4938	T7439	T7438	aux	may,anticipated
R4939	T7581	T7582	compound	cell,culture
R494	T1204	T1201	prep	4,1
R4940	T7582	T7580	pobj	culture,in
R4941	T7583	T7534	punct	.,shown
R4942	T7585	T7586	advmod	Yet,produce
R4943	T7440	T7438	aux	have,anticipated
R4944	T7587	T7586	punct	", ",produce
R4945	T7588	T7586	nsubj	haploinsufficiency,produce
R4946	T7441	T7438	neg	not,anticipated
R4947	T7589	T7588	prep	of,haploinsufficiency
R4948	T7590	T7589	pobj	RanBP2,of
R4949	T7591	T7592	punct	(,work
R495	T1205	T1196	prep	of,domains
R4950	T7442	T7438	auxpass	been,anticipated
R4951	T7592	T7590	parataxis	work,RanBP2
R4952	T7593	T7592	det	this,work
R4953	T7594	T7592	punct	),work
R4954	T7443	T7444	aux	to,linked
R4955	T7595	T7590	cc	and,RanBP2
R4956	T7596	T7590	conj	Nup96,RanBP2
R4957	T7597	T7598	punct	[,58
R4958	T7444	T7438	xcomp	linked,anticipated
R4959	T7598	T7596	parataxis	58,Nup96
R496	T1206	T1205	pobj	RanBP2,of
R4960	T7599	T7598	punct	],58
R4961	T7600	T7586	advmod	predominantly,produce
R4962	T7445	T7444	auxpass	be,linked
R4963	T7601	T7586	punct	", ",produce
R4964	T7602	T7586	advmod	instead,produce
R4965	T7603	T7586	punct	", ",produce
R4966	T7446	T7444	prep	to,linked
R4967	T7604	T7605	npadvmod	CNS,restricted
R4968	T7605	T7607	amod	restricted,deficits
R4969	T7606	T7605	punct	-,restricted
R497	T1207	T1192	prep	with,associate
R4970	T7447	T7448	amod	metabolic,processes
R4971	T7607	T7586	dobj	deficits,produce
R4972	T7608	T7607	prep	in,deficits
R4973	T7609	T7610	compound	energy,metabolism
R4974	T7448	T7446	pobj	processes,to
R4975	T7610	T7608	pobj	metabolism,in
R4976	T7611	T7607	cc	and,deficits
R4977	T7449	T7448	cc	and,processes
R4978	T7612	T7607	conj	alterations,deficits
R4979	T7450	T7451	amod	allied,states
R498	T1208	T1209	det	the,co-receptor
R4980	T7613	T7612	prep	in,alterations
R4981	T7614	T7615	det	the,system
R4982	T7451	T7448	conj	states,processes
R4983	T7615	T7613	pobj	system,in
R4984	T7616	T7615	amod	immune,system
R4985	T7617	T7612	acl	linked,alterations
R4986	T7452	T7451	amod	pathophysiological,states
R4987	T7618	T7617	prep	to,linked
R4988	T7619	T7620	det	the,regulation
R4989	T7453	T7414	punct	.,serve
R499	T1209	T1207	pobj	co-receptor,with
R4990	T7620	T7618	pobj	regulation,to
R4991	T7621	T7620	amod	down,regulation
R4992	T7622	T7620	punct	-,regulation
R4993	T7623	T7620	prep	of,regulation
R4994	T7624	T7625	compound	interferon,β
R4995	T7625	T7627	npadvmod	β,regulated
R4996	T7680	T7670	dobj	stage,set
R4997	T7626	T7625	punct	-,β
R4998	T7627	T7629	amod	regulated,proteins
R4999	T7628	T7627	punct	-,regulated
R5	T234	T235	nmod	Cox11,Activities
R50	T283	T270	punct	.,identified
R500	T1210	T1209	amod	nuclear,co-receptor
R5000	T7629	T7623	pobj	proteins,of
R5001	T7630	T7620	cc	and,regulation
R5002	T7681	T7682	aux	to,probe
R5003	T7631	T7632	amod	increased,susceptibility
R5004	T7632	T7620	conj	susceptibility,regulation
R5005	T7633	T7632	prep	of,susceptibility
R5006	T7682	T7670	advcl	probe,set
R5007	T7634	T7635	amod	viral,infection
R5008	T7635	T7633	pobj	infection,of
R5009	T7636	T7617	punct	", ",linked
R501	T1211	T1209	compound	import,co-receptor
R5010	T7683	T7684	amod	novel,pathways
R5011	T7637	T7617	advmod	respectively,linked
R5012	T7638	T7586	punct	.,produce
R5013	T7684	T7682	dobj	pathways,probe
R5014	T7640	T7641	mark	While,remain
R5015	T7641	T7649	advcl	remain,result
R5016	T7685	T7684	amod	molecular,pathways
R5017	T7642	T7643	det	these,disparities
R5018	T7643	T7641	nsubj	disparities,remain
R5019	T7644	T7643	amod	apparent,disparities
R502	T1212	T1209	punct	", ",co-receptor
R5020	T7686	T7682	prep	in,probe
R5021	T7645	T7643	compound	outcome,disparities
R5022	T7646	T7643	prep	among,disparities
R5023	T7647	T7648	amod	experimental,systems
R5024	T7687	T7688	amod	various,contexts
R5025	T7648	T7646	pobj	systems,among
R5026	T7650	T7641	acomp	unclear,remain
R5027	T7688	T7686	pobj	contexts,in
R5028	T7651	T7649	punct	", ",result
R5029	T7652	T7649	nsubj	they,result
R503	T1213	T1214	compound	importin,β
R5030	T7653	T7649	aux	could,result
R5031	T7689	T7688	amod	physiological,contexts
R5032	T7654	T7649	advmod	potentially,result
R5033	T7655	T7649	prep	from,result
R5034	T7656	T7655	pobj	variations,from
R5035	T7690	T7689	cc	and,physiological
R5036	T7657	T7656	prep	in,variations
R5037	T7658	T7657	pobj	redundancies,in
R5038	T7691	T7689	conj	genetic,physiological
R5039	T7659	T7658	cc	and,redundancies
R504	T1214	T1209	appos	β,co-receptor
R5040	T7660	T7661	amod	compensatory,mechanisms
R5041	T7661	T7658	conj	mechanisms,redundancies
R5042	T7662	T7658	amod	inherent,redundancies
R5043	T7692	T7682	punct	", ",probe
R5044	T7663	T7662	prep	to,inherent
R5045	T7664	T7665	det	each,system
R5046	T7665	T7663	pobj	system,to
R5047	T7666	T7665	amod	experimental,system
R5048	T7667	T7649	punct	.,result
R5049	T7693	T7682	cc	and,probe
R505	T1215	T1214	punct	-,β
R5050	T7669	T7670	advmod	Regardless,set
R5051	T7694	T7682	conj	provide,probe
R5052	T7671	T7670	punct	", ",set
R5053	T7672	T7673	det	these,models
R5054	T7673	T7670	nsubj	models,set
R5055	T7695	T7694	advmod	also,provide
R5056	T7674	T7673	amod	genetic,models
R5057	T7675	T7674	cc	and,genetic
R5058	T7676	T7677	amod	whole,animal
R5059	T7696	T7697	det	a,link
R506	T1216	T1217	punct	[,11
R5060	T7677	T7674	conj	animal,genetic
R5061	T7678	T7677	punct	-,animal
R5062	T7679	T7680	det	the,stage
R5063	T7697	T7694	dobj	link,provide
R5064	T7698	T7697	prep	to,link
R5065	T7699	T7700	amod	pathophysiological,processes
R5066	T7700	T7698	pobj	processes,to
R5067	T7701	T7700	acl	underlying,processes
R5068	T7702	T7703	amod	several,diseases
R5069	T7703	T7701	dobj	diseases,underlying
R507	T1217	T1192	parataxis	11,associate
R5070	T7704	T7703	amod	human,diseases
R5071	T7705	T7670	punct	.,set
R5072	T7787	T7788	nmod	Yeast,screening
R5073	T7789	T7790	nummod	two,hybrid
R5074	T7790	T7788	compound	hybrid,screening
R5075	T7791	T7790	punct	-,hybrid
R5076	T7792	T7788	cc	and,screening
R5077	T7793	T7788	conj	assays,screening
R5078	T7794	T7788	punct	.,screening
R5079	T7796	T7797	det	The,LD
R508	T1218	T1217	nummod	10,11
R5080	T7797	T7798	nsubjpass	LD,subcloned
R5081	T7799	T7797	prep	of,LD
R5082	T7800	T7801	amod	human,RanBP2
R5083	T7801	T7799	pobj	RanBP2,of
R5084	T7802	T7803	punct	(,62
R5085	T7803	T7797	parataxis	62,LD
R5086	T7804	T7803	nmod	residues,62
R5087	T7805	T7806	punct	–,711
R5088	T7806	T7803	prep	711,62
R5089	T7807	T7803	punct	),62
R509	T1219	T1217	punct	",",11
R5090	T7808	T7798	auxpass	was,subcloned
R5091	T7809	T7798	prep	in,subcloned
R5092	T7810	T7809	punct	-,in
R5093	T7811	T7809	pobj	frame,in
R5094	T7812	T7798	prep	into,subcloned
R5095	T7813	T7814	det	the,domain
R5096	T7814	T7812	pobj	domain,into
R5097	T7815	T7814	compound	GAL4,domain
R5098	T7816	T7817	compound	DNA,binding
R5099	T7817	T7814	compound	binding,domain
R51	T285	T286	advmod	Yet,remain
R510	T1220	T1217	punct	],11
R5100	T7818	T7817	punct	-,binding
R5101	T7819	T7814	prep	of,domain
R5102	T7820	T7821	compound	bait,vector
R5103	T7821	T7819	pobj	vector,of
R5104	T7822	T7821	punct	", ",vector
R5105	T7823	T7821	conj	pBUTE,vector
R5106	T7824	T7823	punct	(,pBUTE
R5107	T7825	T7826	det	a,version
R5108	T7826	T7823	appos	version,pBUTE
R5109	T7827	T7828	npadvmod	kanamycin,resistant
R511	T1221	T1192	punct	", ",associate
R5110	T7828	T7826	amod	resistant,version
R5111	T7829	T7828	punct	-,resistant
R5112	T7830	T7826	prep	of,version
R5113	T7831	T7832	compound	GAL4,vector
R5114	T7832	T7830	pobj	vector,of
R5115	T7833	T7832	compound	bait,vector
R5116	T7834	T7832	appos	pGBDUC1,vector
R5117	T7835	T7823	punct	),pBUTE
R5118	T7836	T7837	punct	[,62
R5119	T7837	T7823	parataxis	62,pBUTE
R512	T1222	T1192	cc	and,associate
R5120	T7838	T7837	punct	],62
R5121	T7839	T7823	cc	and,pBUTE
R5122	T7840	T7841	compound	HybriZAP,vector
R5123	T7841	T7823	conj	vector,pBUTE
R5124	T7842	T7843	compound	pBD,GAL4
R5125	T7843	T7841	compound	GAL4,vector
R5126	T7844	T7843	punct	-,GAL4
R5127	T7845	T7846	punct	(,Stratagene
R5128	T7846	T7841	parataxis	Stratagene,vector
R5129	T7847	T7846	punct	", ",Stratagene
R513	T1223	T1224	nsubj	antibodies,inhibit
R5130	T7848	T7849	compound	La,Jolla
R5131	T7849	T7846	npadvmod	Jolla,Stratagene
R5132	T7850	T7846	punct	", ",Stratagene
R5133	T7851	T7846	npadvmod	California,Stratagene
R5134	T7852	T7846	punct	", ",Stratagene
R5135	T7853	T7854	compound	United,States
R5136	T7854	T7846	npadvmod	States,Stratagene
R5137	T7855	T7846	punct	),Stratagene
R5138	T7856	T7798	punct	.,subcloned
R5139	T7858	T7859	det	The,former
R514	T1224	T1192	conj	inhibit,associate
R5140	T7859	T7860	nsubjpass	former,used
R5141	T7861	T7860	auxpass	was,used
R5142	T7862	T7863	aux	to,screen
R5143	T7863	T7860	advcl	screen,used
R5144	T7864	T7865	punct	~,million
R5145	T7865	T7867	nummod	million,clones
R5146	T7866	T7865	compound	18,million
R5147	T7867	T7863	dobj	clones,screen
R5148	T7868	T7867	prep	via,clones
R5149	T7869	T7868	pobj	mating,via
R515	T1225	T1223	prep	against,antibodies
R5150	T7870	T7867	prep	from,clones
R5151	T7871	T7872	nmod	murine,libraries
R5152	T7872	T7870	pobj	libraries,from
R5153	T7873	T7874	quantmod	9,10
R5154	T7874	T7877	nummod	10,d
R5155	T7875	T7874	punct	-,10
R5156	T7876	T7874	quantmod	to,10
R5157	T7877	T7879	npadvmod	d,old
R5158	T7878	T7874	punct	-,10
R5159	T7879	T7872	amod	old,libraries
R516	T1226	T1225	pobj	RanBP2,against
R5160	T7880	T7879	punct	-,old
R5161	T7881	T7872	nmod	embryo,libraries
R5162	T7882	T7881	cc	and,embryo
R5163	T7883	T7881	conj	brain,embryo
R5164	T7884	T7872	compound	cDNA,libraries
R5165	T7885	T7872	prep	at,libraries
R5166	T7886	T7887	det	the,Facility
R5167	T7887	T7885	pobj	Facility,at
R5168	T7888	T7887	compound	Molecular,Facility
R5169	T7889	T7887	compound	Interaction,Facility
R517	T1227	T1228	det	the,pathway
R5170	T7890	T7887	punct	(,Facility
R5171	T7891	T7887	appos	MIF,Facility
R5172	T7892	T7887	punct	),Facility
R5173	T7893	T7887	punct	", ",Facility
R5174	T7894	T7887	npadvmod	University,Facility
R5175	T7895	T7894	prep	of,University
R5176	T7896	T7895	pobj	Wisconsin,of
R5177	T7897	T7894	punct	", ",University
R5178	T7898	T7894	npadvmod	Madison,University
R5179	T7899	T7894	punct	", ",University
R518	T1228	T1224	dobj	pathway,inhibit
R5180	T7900	T7894	npadvmod	Wisconsin,University
R5181	T7901	T7860	punct	.,used
R5182	T7903	T7904	det	The,latter
R5183	T7904	T7905	nsubjpass	latter,used
R5184	T7906	T7905	auxpass	was,used
R5185	T7907	T7908	aux	to,screen
R5186	T7908	T7905	advcl	screen,used
R5187	T7909	T7908	prep	via,screen
R5188	T7910	T7909	pobj	transformation,via
R5189	T7911	T7910	prep	of,transformation
R519	T1229	T1230	amod	nuclear,import
R5190	T7912	T7913	punct	~,million
R5191	T7913	T7915	nummod	million,clones
R5192	T7914	T7913	compound	5,million
R5193	T7915	T7911	pobj	clones,of
R5194	T7916	T7915	prep	from,clones
R5195	T7917	T7918	amod	bovine,libraries
R5196	T7918	T7916	pobj	libraries,from
R5197	T7919	T7918	amod	retinal,libraries
R5198	T7920	T7918	compound	cDNA,libraries
R5199	T7921	T7922	punct	[,32
R52	T287	T286	punct	", ",remain
R520	T1230	T1228	compound	import,pathway
R5200	T7922	T7908	parataxis	32,screen
R5201	T7923	T7922	punct	],32
R5202	T7924	T7905	punct	.,used
R5203	T7926	T7927	det	The,screens
R5204	T7927	T7928	nsubj	screens,generated
R5205	T7929	T7930	nummod	six,clones
R5206	T7930	T7928	dobj	clones,generated
R5207	T7931	T7928	punct	.,generated
R5208	T7933	T7934	nummod	One,clones
R5209	T7934	T7940	nsubjpass	clones,isolated
R521	T1231	T1228	prep	in,pathway
R5210	T7935	T7933	cc	and,One
R5211	T7936	T7933	conj	two,One
R5212	T7937	T7934	nmod	in,clones
R5213	T7938	T7937	punct	-,in
R5214	T7939	T7937	pobj	frame,in
R5215	T7941	T7940	auxpass	were,isolated
R5216	T7942	T7940	advmod	independently,isolated
R5217	T7943	T7940	prep	from,isolated
R5218	T7944	T7945	det	the,libraries
R5219	T7945	T7943	pobj	libraries,from
R522	T1232	T1233	compound	HeLa,cells
R5220	T7946	T7945	amod	embryonic,libraries
R5221	T7947	T7946	cc	and,embryonic
R5222	T7948	T7946	conj	adult,embryonic
R5223	T7949	T7945	compound	brain,libraries
R5224	T7950	T7940	punct	", ",isolated
R5225	T7951	T7940	advmod	respectively,isolated
R5226	T7952	T7940	punct	", ",isolated
R5227	T7953	T7940	cc	and,isolated
R5228	T7954	T7955	det	the,interactions
R5229	T7955	T7956	nsubjpass	interactions,validated
R523	T1233	T1231	pobj	cells,in
R5230	T7956	T7940	conj	validated,isolated
R5231	T7957	T7956	auxpass	were,validated
R5232	T7958	T7956	punct	.,validated
R5233	T7960	T7961	det	The,clones
R5234	T7961	T7963	nsubj	clones,encoded
R5235	T7962	T7961	nummod	three,clones
R5236	T7964	T7963	dobj	Cox11,encoded
R5237	T7965	T7963	punct	.,encoded
R5238	T7967	T7968	nsubjpass	Interactions,quantified
R5239	T7969	T7967	prep	between,Interactions
R524	T1234	T1235	punct	[,3
R5240	T7970	T7969	pobj	Cox11,between
R5241	T7971	T7970	punct	", ",Cox11
R5242	T7972	T7970	conj	LD,Cox11
R5243	T7973	T7972	punct	", ",LD
R5244	T7974	T7972	cc	and,LD
R5245	T7975	T7972	conj	subdomains,LD
R5246	T7976	T7975	advmod	thereof,subdomains
R5247	T7977	T7968	auxpass	were,quantified
R5248	T7978	T7968	prep	by,quantified
R5249	T7979	T7980	amod	liquid,galactosidase
R525	T1235	T1224	parataxis	3,inhibit
R5250	T7980	T7978	pobj	galactosidase,by
R5251	T7981	T7980	compound	β,galactosidase
R5252	T7982	T7980	punct	-,galactosidase
R5253	T7983	T7984	punct	(,Biosystems
R5254	T7984	T7980	parataxis	Biosystems,galactosidase
R5255	T7985	T7984	compound	Applied,Biosystems
R5256	T7986	T7984	punct	", ",Biosystems
R5257	T7987	T7988	compound	Foster,City
R5258	T7988	T7984	npadvmod	City,Biosystems
R5259	T7989	T7984	punct	", ",Biosystems
R526	T1236	T1235	punct	],3
R5260	T7990	T7984	npadvmod	California,Biosystems
R5261	T7991	T7984	punct	", ",Biosystems
R5262	T7992	T7993	compound	United,States
R5263	T7993	T7984	npadvmod	States,Biosystems
R5264	T7994	T7984	punct	),Biosystems
R5265	T7995	T7980	cc	and,galactosidase
R5266	T7996	T7997	compound	growth,assays
R5267	T7997	T7980	conj	assays,galactosidase
R5268	T7998	T7999	punct	[,63
R5269	T7999	T7968	parataxis	63,quantified
R527	T1237	T1224	punct	;,inhibit
R5270	T8000	T7999	punct	],63
R5271	T8001	T7968	punct	.,quantified
R5272	T8003	T8004	det	The,speed
R5273	T8004	T8008	nsubjpass	speed,determined
R5274	T8005	T8004	nmod	maximum,speed
R5275	T8006	T8004	amod	specific,speed
R5276	T8007	T8004	compound	growth,speed
R5277	T8009	T8004	punct	(,speed
R5278	T8010	T8004	appos	μmax,speed
R5279	T8011	T8008	punct	),determined
R528	T1238	T1224	cc	but,inhibit
R5280	T8012	T8008	auxpass	was,determined
R5281	T8013	T8008	prep	by,determined
R5282	T8014	T8013	pcomp	calculating,by
R5283	T8015	T8016	nsubj	μmax,x t
R5284	T8016	T8014	ccomp	x t,calculating
R5285	T8017	T8016	punct	=,x t
R5286	T8018	T8016	punct	(,x t
R5287	T8019	T8016	nmod	ln,x t
R5288	T8020	T8016	punct	(,x t
R5289	T8021	T8016	punct	),x t
R529	T1239	T1240	predet	such,role
R5290	T8022	T8016	punct	−,x t
R5291	T8023	T8024	nmod	ln,x 0
R5292	T8024	T8016	appos	x 0,x t
R5293	T8025	T8024	punct	(,x 0
R5294	T8026	T8016	punct	),x t
R5295	T8027	T8016	punct	),x t
R5296	T8028	T8016	punct	/,x t
R5297	T8029	T8016	appos	t,x t
R5298	T8030	T8016	punct	", ",x t
R5299	T8031	T8032	advmod	where,is
R53	T288	T289	det	the,significance
R530	T1240	T1242	nsubj	role,seems
R5300	T8032	T8016	advcl	is,x t
R5301	T8033	T8032	nsubj	x t,is
R5302	T8034	T8035	det	the,OD600
R5303	T8035	T8032	attr	OD600,is
R5304	T8036	T8035	prep	of,OD600
R5305	T8037	T8038	det	the,culture
R5306	T8038	T8036	pobj	culture,of
R5307	T8039	T8035	prep	at,OD600
R5308	T8040	T8041	nsubj	t,t
R5309	T8041	T8039	pcomp	t,at
R531	T1241	T1240	det	a,role
R5310	T8042	T8041	punct	=,t
R5311	T8043	T8032	punct	", ",is
R5312	T8044	T8045	nsubj	x 0,is
R5313	T8045	T8032	conj	is,is
R5314	T8046	T8047	det	the,OD600
R5315	T8047	T8045	attr	OD600,is
R5316	T8048	T8045	prep	at,is
R5317	T8049	T8050	nsubj	t,0
R5318	T8050	T8048	pcomp	0,at
R5319	T8051	T8050	punct	=,0
R532	T1242	T1224	conj	seems,inhibit
R5320	T8052	T8045	cc	and,is
R5321	T8053	T8054	nsubj	t,is
R5322	T8054	T8045	conj	is,is
R5323	T8055	T8056	det	the,time
R5324	T8056	T8054	attr	time,is
R5325	T8057	T8056	prep	between,time
R5326	T8058	T8057	pobj	x 0,between
R5327	T8059	T8058	cc	and,x 0
R5328	T8060	T8058	conj	x t,x 0
R5329	T8061	T8008	punct	.,determined
R533	T1243	T1242	oprd	dispensable,seems
R5330	T8063	T8064	nsubjpass	Assays,performed
R5331	T8065	T8064	auxpass	were,performed
R5332	T8066	T8064	prep	with,performed
R5333	T8067	T8068	nummod	three,clones
R5334	T8068	T8066	pobj	clones,with
R5335	T8069	T8068	amod	independent,clones
R5336	T8070	T8068	cc	and,clones
R5337	T8071	T8072	nummod	three,samples
R5338	T8072	T8068	conj	samples,clones
R5339	T8073	T8072	prep	of,samples
R534	T1244	T1242	prep	in,seems
R5340	T8074	T8075	det	each,clone
R5341	T8075	T8073	pobj	clone,of
R5342	T8076	T8064	punct	", ",performed
R5343	T8077	T8078	det	the,results
R5344	T8078	T8079	nsubjpass	results,averaged
R5345	T8079	T8064	conj	averaged,performed
R5346	T8080	T8079	auxpass	were,averaged
R5347	T8081	T8079	punct	", ",averaged
R5348	T8082	T8079	cc	and,averaged
R5349	T8083	T8084	det	the,deviations
R535	T1245	T1246	compound	Xenopus,oocytes
R5350	T8084	T8086	nsubj	deviations,calculated
R5351	T8085	T8084	amod	standard,deviations
R5352	T8086	T8079	conj	calculated,averaged
R5353	T8087	T8086	punct	.,calculated
R5354	T8119	T8120	npadvmod	Site,directed
R5355	T8120	T8122	amod	directed,mutagenesis
R5356	T8121	T8120	punct	-,directed
R5357	T8123	T8120	cc	and,directed
R5358	T8124	T8120	conj	deletion,directed
R5359	T8125	T8122	cc	and,mutagenesis
R536	T1246	T1244	pobj	oocytes,in
R5360	T8126	T8127	compound	plasmid,construction
R5361	T8127	T8122	conj	construction,mutagenesis
R5362	T8128	T8127	punct	.,construction
R5363	T8130	T8131	compound	Deletion,mutagenesis
R5364	T8131	T8132	nsubjpass	mutagenesis,carried
R5365	T8133	T8132	auxpass	was,carried
R5366	T8134	T8132	prt	out,carried
R5367	T8135	T8132	prep	with,carried
R5368	T8136	T8135	pobj	pairs,with
R5369	T8137	T8136	prep	of,pairs
R537	T1247	T1248	punct	[,12
R5370	T8138	T8137	pobj	primers,of
R5371	T8139	T8138	prep	against,primers
R5372	T8140	T8139	pobj	domains,against
R5373	T8141	T8140	prep	of,domains
R5374	T8142	T8141	pobj	interest,of
R5375	T8143	T8140	acl	described,domains
R5376	T8144	T8143	prep	in,described
R5377	T8145	T8146	det	the,legends
R5378	T8146	T8144	pobj	legends,in
R5379	T8147	T8146	compound	figure,legends
R538	T1248	T1242	parataxis	12,seems
R5380	T8148	T8132	punct	.,carried
R5381	T8150	T8151	compound	PCR,products
R5382	T8151	T8152	nsubjpass	products,subcloned
R5383	T8153	T8152	auxpass	were,subcloned
R5384	T8154	T8152	prep	into,subcloned
R5385	T8155	T8156	compound	pGEX,KG
R5386	T8156	T8154	pobj	KG,into
R5387	T8157	T8156	punct	-,KG
R5388	T8158	T8159	punct	[,64
R5389	T8159	T8156	parataxis	64,KG
R539	T1249	T1248	punct	],12
R5390	T8160	T8159	punct	],64
R5391	T8161	T8156	punct	", ",KG
R5392	T8162	T8163	compound	HybriZAP,vector
R5393	T8163	T8156	conj	vector,KG
R5394	T8164	T8165	compound	pBD,GAL4
R5395	T8165	T8163	compound	GAL4,vector
R5396	T8166	T8165	punct	-,GAL4
R5397	T8167	T8168	punct	(,Stratagene
R5398	T8168	T8163	parataxis	Stratagene,vector
R5399	T8169	T8168	punct	),Stratagene
R54	T289	T286	nsubj	significance,remain
R540	T1250	T1242	punct	.,seems
R5400	T8170	T8163	punct	", ",vector
R5401	T8171	T8163	cc	and,vector
R5402	T8172	T8173	nmod	pBUTE,vectors
R5403	T8173	T8163	conj	vectors,vector
R5404	T8174	T8175	punct	[,62
R5405	T8175	T8172	parataxis	62,pBUTE
R5406	T8176	T8175	punct	],62
R5407	T8177	T8152	punct	.,subcloned
R5408	T8179	T8180	compound	GST,LDZIP
R5409	T8180	T8182	nsubj	LDZIP,comprised
R541	T1252	T1253	prep	In,associates
R5410	T8181	T8180	punct	-,LDZIP
R5411	T8183	T8180	advmod	alone,LDZIP
R5412	T8184	T8185	nmod	residues,447
R5413	T8185	T8182	dobj	447,comprised
R5414	T8186	T8187	punct	–,483
R5415	T8187	T8185	prep	483,447
R5416	T8188	T8185	prep	of,447
R5417	T8189	T8190	amod	human,RanBP2
R5418	T8190	T8188	pobj	RanBP2,of
R5419	T8191	T8182	punct	.,comprised
R542	T1254	T1252	pobj	addition,In
R5420	T8257	T8258	nmod	GST,assays
R5421	T8259	T8258	amod	pull,assays
R5422	T8260	T8259	punct	-,pull
R5423	T8261	T8259	prt	down,pull
R5424	T8262	T8259	cc	and,pull
R5425	T8263	T8259	conj	immunoprecipitation,pull
R5426	T8264	T8258	punct	.,assays
R5427	T8266	T8267	npadvmod	CHAPS,solubilized
R5428	T8267	T8269	amod	solubilized,extracts
R5429	T8268	T8267	punct	-,solubilized
R543	T1255	T1253	punct	", ",associates
R5430	T8269	T8271	nsubjpass	extracts,prepared
R5431	T8270	T8269	amod	retinal,extracts
R5432	T8272	T8269	punct	", ",extracts
R5433	T8273	T8269	conj	expression,extracts
R5434	T8274	T8273	punct	", ",expression
R5435	T8275	T8273	cc	and,expression
R5436	T8276	T8273	conj	purification,expression
R5437	T8277	T8276	prep	of,purification
R5438	T8278	T8279	npadvmod	GST,fused
R5439	T8279	T8281	amod	fused,constructs
R544	T1256	T1257	det	the,combination
R5440	T8280	T8279	punct	-,fused
R5441	T8281	T8277	pobj	constructs,of
R5442	T8282	T8271	auxpass	were,prepared
R5443	T8283	T8284	mark	as,described
R5444	T8284	T8271	advcl	described,prepared
R5445	T8285	T8284	advmod	previously,described
R5446	T8286	T8287	punct	[,65
R5447	T8287	T8271	parataxis	65,prepared
R5448	T8288	T8287	punct	],65
R5449	T8289	T8271	punct	.,prepared
R545	T1257	T1253	nsubj	combination,associates
R5450	T8291	T8292	nmod	GST,assays
R5451	T8292	T8296	nsubjpass	assays,carried
R5452	T8293	T8292	amod	pull,assays
R5453	T8294	T8293	punct	-,pull
R5454	T8295	T8293	prt	down,pull
R5455	T8297	T8296	auxpass	were,carried
R5456	T8298	T8296	prt	out,carried
R5457	T8299	T8296	prep	with,carried
R5458	T8300	T8301	quantmod	0.5,2.2
R5459	T8301	T8303	nummod	2.2,μM
R546	T1258	T1257	prep	of,combination
R5460	T8302	T8301	punct	–,2.2
R5461	T8303	T8299	pobj	μM,with
R5462	T8304	T8303	prep	of,μM
R5463	T8305	T8306	npadvmod	GST,fused
R5464	T8306	T8308	amod	fused,proteins
R5465	T8307	T8306	punct	-,fused
R5466	T8308	T8304	pobj	proteins,of
R5467	T8309	T8310	punct	[,65
R5468	T8310	T8296	parataxis	65,carried
R5469	T8311	T8310	punct	],65
R547	T1259	T1260	det	the,domains
R5470	T8312	T8296	punct	.,carried
R5471	T8314	T8315	nsubjpass	Co-precipitates,resolved
R5472	T8316	T8315	auxpass	were,resolved
R5473	T8317	T8315	prep	on,resolved
R5474	T8318	T8319	compound	SDS,PAGE
R5475	T8319	T8317	pobj	PAGE,on
R5476	T8320	T8319	punct	-,PAGE
R5477	T8321	T8315	cc	and,resolved
R5478	T8322	T8315	conj	analyzed,resolved
R5479	T8323	T8322	prep	by,analyzed
R548	T1260	T1258	pobj	domains,of
R5480	T8324	T8325	compound	Western,blot
R5481	T8325	T8323	pobj	blot,by
R5482	T8326	T8322	prep	with,analyzed
R5483	T8327	T8326	pobj	antibodies,with
R5484	T8328	T8327	acl	described,antibodies
R5485	T8329	T8328	prep	in,described
R5486	T8330	T8331	det	the,section
R5487	T8331	T8329	pobj	section,in
R5488	T8332	T8331	compound	Results,section
R5489	T8333	T8315	punct	.,resolved
R549	T1261	T1262	npadvmod	C,terminal
R5490	T8335	T8336	amod	Unfolded,Cox11
R5491	T8336	T8340	nsubjpass	Cox11,generated
R5492	T8337	T8335	cc	and,Unfolded
R5493	T8338	T8339	advmod	partially,denatured
R5494	T8339	T8335	conj	denatured,Unfolded
R5495	T8341	T8340	auxpass	were,generated
R5496	T8342	T8340	prep	by,generated
R5497	T8343	T8342	pcomp	incubating,by
R5498	T8344	T8345	amod	recombinant,Cox11
R5499	T8345	T8343	dobj	Cox11,incubating
R55	T290	T289	prep	of,significance
R550	T1262	T1260	amod	terminal,domains
R5500	T8346	T8344	cc	and,recombinant
R5501	T8347	T8344	conj	native,recombinant
R5502	T8348	T8340	advmod	overnight,generated
R5503	T8349	T8340	prep	with,generated
R5504	T8350	T8351	nummod	5.6,M
R5505	T8351	T8349	pobj	M,with
R5506	T8352	T8350	cc	and,5.6
R5507	T8353	T8350	conj	7,5.6
R5508	T8354	T8355	compound	guanidine,hydrochloride
R5509	T8355	T8351	appos	hydrochloride,M
R551	T1263	T1262	punct	-,terminal
R5510	T8356	T8355	cc	and,hydrochloride
R5511	T8357	T8355	conj	urea,hydrochloride
R5512	T8358	T8340	punct	", ",generated
R5513	T8359	T8340	advmod	respectively,generated
R5514	T8360	T8340	punct	.,generated
R5515	T8362	T8363	det	The,conformers
R5516	T8363	T8365	nsubjpass	conformers,diluted
R5517	T8364	T8363	compound	Cox11,conformers
R5518	T8366	T8365	auxpass	were,diluted
R5519	T8367	T8365	advmod	then,diluted
R552	T1264	T1260	punct	", ",domains
R5520	T8368	T8369	punct	~,20-fold
R5521	T8369	T8365	advmod	20-fold,diluted
R5522	T8370	T8365	prep	in,diluted
R5523	T8371	T8372	compound	CHAPS,binding
R5524	T8372	T8374	compound	binding,buffer
R5525	T8373	T8372	punct	-,binding
R5526	T8374	T8370	pobj	buffer,in
R5527	T8375	T8374	acl	containing,buffer
R5528	T8376	T8377	compound	GST,LD
R5529	T8377	T8375	dobj	LD,containing
R553	T1265	T1260	appos	RBD4,domains
R5530	T8378	T8377	punct	-,LD
R5531	T8379	T8365	punct	.,diluted
R5532	T8381	T8382	compound	Immunoprecipitation,assays
R5533	T8382	T8383	nsubjpass	assays,performed
R5534	T8384	T8382	prep	with,assays
R5535	T8385	T8386	nummod	5,μg
R5536	T8386	T8384	pobj	μg,with
R5537	T8387	T8386	prep	of,μg
R5538	T8388	T8387	pobj	antibody,of
R5539	T8389	T8382	cc	and,assays
R554	T1266	T1265	cc	and,RBD4
R5540	T8390	T8391	compound	Western,blots
R5541	T8391	T8382	conj	blots,assays
R5542	T8392	T8393	punct	(,ng
R5543	T8393	T8391	parataxis	ng,blots
R5544	T8394	T8395	punct	~,400
R5545	T8395	T8393	nummod	400,ng
R5546	T8396	T8395	quantmod	200,400
R5547	T8397	T8395	punct	–,400
R5548	T8398	T8399	punct	/,ml
R5549	T8399	T8393	prep	ml,ng
R555	T1267	T1265	conj	CY,RBD4
R5550	T8400	T8399	prep	of,ml
R5551	T8401	T8400	pobj	antibody,of
R5552	T8402	T8393	punct	),ng
R5553	T8403	T8383	auxpass	were,performed
R5554	T8404	T8405	advmod	exactly,described
R5555	T8405	T8383	advcl	described,performed
R5556	T8406	T8405	mark	as,described
R5557	T8407	T8405	advmod	previously,described
R5558	T8408	T8409	punct	[,13
R5559	T8409	T8405	parataxis	13,described
R556	T1268	T1260	punct	(,domains
R5560	T8410	T8409	punct	],13
R5561	T8411	T8383	punct	.,performed
R5562	T8483	T8484	nmod	Hexokinase,assay
R5563	T8485	T8483	nummod	I,Hexokinase
R5564	T8486	T8484	punct	.,assay
R5565	T8488	T8489	nmod	Hexokinase,activity
R5566	T8489	T8491	nsubjpass	activity,determined
R5567	T8490	T8488	nummod	I,Hexokinase
R5568	T8492	T8491	auxpass	was,determined
R5569	T8493	T8491	advmod	spectrophotometrically,determined
R557	T1269	T1260	prep	of,domains
R5570	T8494	T8491	prep	at,determined
R5571	T8495	T8496	nummod	25,°C
R5572	T8496	T8494	pobj	°C,at
R5573	T8497	T8491	prep	by,determined
R5574	T8498	T8499	det	the,method
R5575	T8499	T8497	pobj	method,by
R5576	T8500	T8499	prep	of,method
R5577	T8501	T8500	pcomp	coupling,of
R5578	T8502	T8503	det	the,production
R5579	T8503	T8501	dobj	production,coupling
R558	T1270	T1269	pobj	RanBP2,of
R5580	T8504	T8505	nmod	glucose,phosphate
R5581	T8505	T8503	compound	phosphate,production
R5582	T8506	T8505	punct	-,phosphate
R5583	T8507	T8505	nummod	6,phosphate
R5584	T8508	T8505	punct	-,phosphate
R5585	T8509	T8503	prep	via,production
R5586	T8510	T8511	nmod	glucose,phosphate
R5587	T8511	T8515	compound	phosphate,dehydrogenase
R5588	T8512	T8511	punct	-,phosphate
R5589	T8513	T8511	nummod	6,phosphate
R559	T1271	T1253	punct	),associates
R5590	T8514	T8511	punct	-,phosphate
R5591	T8515	T8509	pobj	dehydrogenase,via
R5592	T8516	T8501	prep	with,coupling
R5593	T8517	T8518	det	the,change
R5594	T8518	T8516	pobj	change,with
R5595	T8519	T8518	prep	in,change
R5596	T8520	T8521	det	the,absorbance
R5597	T8521	T8519	pobj	absorbance,in
R5598	T8522	T8521	prep	of,absorbance
R5599	T8523	T8522	pobj	NADPH,of
R56	T291	T292	det	these,interactions
R560	T1272	T1253	punct	", ",associates
R5600	T8524	T8518	prep	at,change
R5601	T8525	T8526	nummod	340,nm
R5602	T8526	T8524	pobj	nm,at
R5603	T8527	T8497	cc	and,by
R5604	T8528	T8529	mark	as,described
R5605	T8529	T8497	conj	described,by
R5606	T8530	T8529	agent	by,described
R5607	T8531	T8532	punct	[,66
R5608	T8532	T8530	pobj	66,by
R5609	T8533	T8491	punct	],determined
R561	T1273	T1253	prep	with,associates
R5610	T8534	T8491	punct	.,determined
R5611	T8536	T8537	det	The,reaction
R5612	T8537	T8538	nsubjpass	reaction,started
R5613	T8539	T8538	auxpass	was,started
R5614	T8540	T8538	agent	by,started
R5615	T8541	T8542	det	the,addition
R5616	T8542	T8540	pobj	addition,by
R5617	T8543	T8542	prep	of,addition
R5618	T8544	T8545	amod	purified,hexokinase
R5619	T8545	T8543	pobj	hexokinase,of
R562	T1274	T1275	det	a,subset
R5620	T8546	T8545	compound	brain,hexokinase
R5621	T8547	T8545	nummod	I,hexokinase
R5622	T8548	T8549	punct	(,μg
R5623	T8549	T8545	parataxis	μg,hexokinase
R5624	T8550	T8549	nummod	0.24,μg
R5625	T8551	T8549	punct	),μg
R5626	T8552	T8553	punct	(,gift
R5627	T8553	T8545	parataxis	gift,hexokinase
R5628	T8554	T8553	prep	from,gift
R5629	T8555	T8556	compound	J.,Wilson
R563	T1275	T1273	pobj	subset,with
R5630	T8556	T8554	pobj	Wilson,from
R5631	T8557	T8553	punct	),gift
R5632	T8558	T8542	prep	to,addition
R5633	T8559	T8560	nummod	1.0,ml
R5634	T8560	T8558	pobj	ml,to
R5635	T8561	T8560	prep	of,ml
R5636	T8562	T8563	compound	reaction,mixture
R5637	T8563	T8561	pobj	mixture,of
R5638	T8564	T8560	acl	containing,ml
R5639	T8565	T8566	nummod	0.05,M
R564	T1276	T1275	prep	of,subset
R5640	T8566	T8567	compound	M,HCl
R5641	T8567	T8564	dobj	HCl,containing
R5642	T8568	T8567	compound	Tris,HCl
R5643	T8569	T8567	punct	-,HCl
R5644	T8570	T8571	punct	(,pH
R5645	T8571	T8567	parataxis	pH,HCl
R5646	T8572	T8571	nummod	8.5,pH
R5647	T8573	T8571	punct	),pH
R5648	T8574	T8567	punct	", ",HCl
R5649	T8575	T8576	nummod	7.4,mM
R565	T1277	T1278	compound	G,protein
R5650	T8576	T8577	compound	mM,MgCl2
R5651	T8577	T8567	conj	MgCl2,HCl
R5652	T8578	T8577	punct	", ",MgCl2
R5653	T8579	T8580	nummod	6.6,mM
R5654	T8580	T8581	compound	mM,ATP
R5655	T8581	T8577	conj	ATP,MgCl2
R5656	T8582	T8581	punct	", ",ATP
R5657	T8583	T8584	nummod	0.65,mM
R5658	T8584	T8585	compound	mM,NADP
R5659	T8585	T8581	conj	NADP,ATP
R566	T1278	T1279	npadvmod	protein,coupled
R5660	T8586	T8585	punct	", ",NADP
R5661	T8587	T8588	nummod	11.1,mM
R5662	T8588	T8589	compound	mM,monothioglycerol
R5663	T8589	T8585	conj	monothioglycerol,NADP
R5664	T8590	T8589	punct	", ",monothioglycerol
R5665	T8591	T8589	cc	and,monothioglycerol
R5666	T8592	T8593	nummod	1,unit
R5667	T8593	T8589	conj	unit,monothioglycerol
R5668	T8594	T8593	prep	of,unit
R5669	T8595	T8596	nmod	glucose,phosphate
R567	T1279	T1281	amod	coupled,receptors
R5670	T8596	T8599	compound	phosphate,dehydrogenase
R5671	T8597	T8596	nummod	6,phosphate
R5672	T8598	T8596	punct	-,phosphate
R5673	T8599	T8594	pobj	dehydrogenase,of
R5674	T8600	T8538	punct	.,started
R5675	T8602	T8603	prep	In,incubated
R5676	T8604	T8605	det	the,case
R5677	T8605	T8602	pobj	case,In
R5678	T8606	T8605	prep	of,case
R5679	T8607	T8608	compound	hexokinase,activity
R568	T1280	T1279	punct	-,coupled
R5680	T8608	T8606	pobj	activity,of
R5681	T8609	T8608	acl	measured,activity
R5682	T8610	T8609	prep	in,measured
R5683	T8611	T8612	det	the,presence
R5684	T8612	T8610	pobj	presence,in
R5685	T8613	T8612	prep	of,presence
R5686	T8614	T8613	pobj	Cox11,of
R5687	T8615	T8614	cc	and,Cox11
R5688	T8616	T8614	conj	LD,Cox11
R5689	T8617	T8603	punct	", ",incubated
R569	T1281	T1276	pobj	receptors,of
R5690	T8618	T8619	det	the,HKI
R5691	T8619	T8603	nsubjpass	HKI,incubated
R5692	T8620	T8619	amod	purified,HKI
R5693	T8621	T8603	auxpass	was,incubated
R5694	T8622	T8603	prep	with,incubated
R5695	T8623	T8624	det	the,proteins
R5696	T8624	T8622	pobj	proteins,with
R5697	T8625	T8624	amod	recombinant,proteins
R5698	T8626	T8603	prep	for,incubated
R5699	T8627	T8628	nummod	15,min
R57	T292	T290	pobj	interactions,of
R570	T1282	T1275	punct	", ",subset
R5700	T8628	T8626	pobj	min,for
R5701	T8629	T8603	prep	at,incubated
R5702	T8630	T8631	nummod	4,°C
R5703	T8631	T8629	pobj	°C,at
R5704	T8632	T8603	prep	before,incubated
R5705	T8633	T8632	pobj	measurement,before
R5706	T8634	T8633	prep	of,measurement
R5707	T8635	T8636	det	the,activity
R5708	T8636	T8634	pobj	activity,of
R5709	T8637	T8603	punct	.,incubated
R571	T1283	T1284	det	the,opsin
R5710	T8639	T8640	det	The,data
R5711	T8640	T8641	nsubjpass	data,fitted
R5712	T8642	T8641	auxpass	were,fitted
R5713	T8643	T8641	advmod	directly,fitted
R5714	T8644	T8641	prep	into,fitted
R5715	T8645	T8646	det	the,equation
R5716	T8646	T8644	pobj	equation,into
R5717	T8647	T8648	compound	Michaelis,Menten
R5718	T8648	T8646	compound	Menten,equation
R5719	T8649	T8648	punct	-,Menten
R572	T1284	T1275	appos	opsin,subset
R5720	T8650	T8641	advcl	using,fitted
R5721	T8651	T8650	dobj	SIGMAPLOT,using
R5722	T8652	T8653	punct	(,Science
R5723	T8653	T8651	parataxis	Science,SIGMAPLOT
R5724	T8654	T8653	compound	SPSS,Science
R5725	T8655	T8653	punct	),Science
R5726	T8656	T8641	punct	.,fitted
R5727	T8701	T8700	punct	.,Antibodies
R5728	T8703	T8704	compound	Rabbit,antisera
R5729	T8704	T8705	nsubjpass	antisera,raised
R573	T1285	T1286	amod	red,green
R5730	T8706	T8705	auxpass	were,raised
R5731	T8707	T8705	prep	against,raised
R5732	T8708	T8709	det	the,Cox11
R5733	T8709	T8707	pobj	Cox11,against
R5734	T8710	T8709	amod	recombinant,Cox11
R5735	T8711	T8709	amod	murine,Cox11
R5736	T8712	T8713	punct	(,40
R5737	T8713	T8707	parataxis	40,against
R5738	T8714	T8713	nmod	residues,40
R5739	T8715	T8716	punct	–,275
R574	T1286	T1284	amod	green,opsin
R5740	T8716	T8713	prep	275,40
R5741	T8717	T8713	punct	),40
R5742	T8718	T8705	cc	and,raised
R5743	T8719	T8720	dep	affinity,purified
R5744	T8720	T8705	conj	purified,raised
R5745	T8721	T8720	punct	-,purified
R5746	T8722	T8720	prep	under,purified
R5747	T8723	T8724	compound	non-denaturing,conditions
R5748	T8724	T8722	pobj	conditions,under
R5749	T8725	T8726	punct	(,Stereogene
R575	T1287	T1286	punct	/,green
R5750	T8726	T8724	parataxis	Stereogene,conditions
R5751	T8727	T8726	punct	),Stereogene
R5752	T8728	T8729	mark	as,described
R5753	T8729	T8720	advcl	described,purified
R5754	T8730	T8729	advmod	previously,described
R5755	T8731	T8732	punct	[,13
R5756	T8732	T8729	parataxis	13,described
R5757	T8733	T8732	punct	],13
R5758	T8734	T8705	punct	.,raised
R5759	T8736	T8737	compound	Cox11,antibodies
R576	T1288	T1284	punct	", ",opsin
R5760	T8737	T8738	nsubjpass	antibodies,used
R5761	T8739	T8738	auxpass	were,used
R5762	T8740	T8738	prep	at,used
R5763	T8741	T8742	nummod	200,ng
R5764	T8742	T8740	pobj	ng,at
R5765	T8743	T8744	punct	/,ml
R5766	T8744	T8742	prep	ml,ng
R5767	T8745	T8738	prep	for,used
R5768	T8746	T8747	compound	Western,analysis
R5769	T8747	T8745	pobj	analysis,for
R577	T1289	T1284	acl	expressed,opsin
R5770	T8748	T8738	punct	.,used
R5771	T8750	T8751	det	The,Hsp70
R5772	T8751	T8754	nsubj	Hsp70,was
R5773	T8752	T8751	amod	monoclonal,Hsp70
R5774	T8753	T8751	compound	antibody,Hsp70
R5775	T8755	T8754	prep	from,was
R5776	T8756	T8757	compound	Affinity,Bioreagents
R5777	T8757	T8755	pobj	Bioreagents,from
R5778	T8758	T8759	punct	(,Golden
R5779	T8759	T8757	parataxis	Golden,Bioreagents
R578	T1290	T1289	prep	in,expressed
R5780	T8760	T8759	punct	", ",Golden
R5781	T8761	T8759	npadvmod	Colorado,Golden
R5782	T8762	T8759	punct	", ",Golden
R5783	T8763	T8764	compound	United,States
R5784	T8764	T8759	npadvmod	States,Golden
R5785	T8765	T8759	punct	),Golden
R5786	T8766	T8754	cc	and,was
R5787	T8767	T8768	nsubjpass	antibodies,described
R5788	T8768	T8754	conj	described,was
R5789	T8769	T8767	prep	against,antibodies
R579	T1291	T1292	amod	photosensory,neurons
R5790	T8770	T8771	det	the,domains
R5791	T8771	T8769	pobj	domains,against
R5792	T8772	T8771	nmod	KBD,domains
R5793	T8773	T8774	punct	(,JX2
R5794	T8774	T8772	parataxis	JX2,KBD
R5795	T8775	T8774	punct	),JX2
R5796	T8776	T8772	cc	and,KBD
R5797	T8777	T8772	conj	ZnF,KBD
R5798	T8778	T8771	prep	of,domains
R5799	T8779	T8778	pobj	RanBP2,of
R58	T293	T292	prep	with,interactions
R580	T1292	T1290	pobj	neurons,in
R5800	T8780	T8768	aux	have,described
R5801	T8781	T8768	auxpass	been,described
R5802	T8782	T8783	punct	[,16
R5803	T8783	T8768	parataxis	16,described
R5804	T8784	T8783	nummod	13,16
R5805	T8785	T8783	punct	",",16
R5806	T8786	T8783	punct	],16
R5807	T8787	T8768	punct	.,described
R5808	T8789	T8790	amod	Polyclonal,antibodies
R5809	T8790	T8793	nsubjpass	antibodies,provided
R581	T1293	T1253	cc	and,associates
R5810	T8791	T8789	cc	and,Polyclonal
R5811	T8792	T8789	conj	monoclonal,Polyclonal
R5812	T8794	T8790	prep	against,antibodies
R5813	T8795	T8794	pobj	Hexokinase,against
R5814	T8796	T8795	nmod	I,Hexokinase
R5815	T8797	T8793	auxpass	were,provided
R5816	T8798	T8793	agent	by,provided
R5817	T8799	T8800	compound	J.,Wilson
R5818	T8800	T8798	pobj	Wilson,by
R5819	T8801	T8793	punct	.,provided
R582	T1294	T1253	conj	enhances,associates
R5820	T8803	T8804	det	The,mAb414
R5821	T8804	T8805	nsubjpass	mAb414,purchased
R5822	T8806	T8805	auxpass	was,purchased
R5823	T8807	T8805	prep	from,purchased
R5824	T8808	T8807	pobj	Abcam,from
R5825	T8809	T8810	punct	(,Cambridge
R5826	T8810	T8808	parataxis	Cambridge,Abcam
R5827	T8811	T8810	punct	", ",Cambridge
R5828	T8812	T8810	npadvmod	Massachusetts,Cambridge
R5829	T8813	T8810	punct	", ",Cambridge
R583	T1295	T1296	nmod	opsin,production
R5830	T8814	T8815	compound	United,States
R5831	T8815	T8810	npadvmod	States,Cambridge
R5832	T8816	T8810	punct	),Cambridge
R5833	T8817	T8805	punct	.,purchased
R5834	T8884	T8883	cc	and,Immunocytochemistry
R5835	T8885	T8883	conj	microscopy,Immunocytochemistry
R5836	T8886	T8885	punct	.,microscopy
R5837	T8888	T8889	compound	Retina,dissections
R5838	T8889	T8890	nsubjpass	dissections,carried
R5839	T8891	T8889	punct	", ",dissections
R584	T1296	T1294	dobj	production,enhances
R5840	T8892	T8893	amod	radial,cryosections
R5841	T8893	T8889	conj	cryosections,dissections
R5842	T8894	T8895	punct	(,μm
R5843	T8895	T8893	parataxis	μm,cryosections
R5844	T8896	T8897	punct	~,6
R5845	T8897	T8895	nummod	6,μm
R5846	T8898	T8895	punct	),μm
R5847	T8899	T8893	punct	", ",cryosections
R5848	T8900	T8893	cc	and,cryosections
R5849	T8901	T8902	compound	immunohistochemistry,procedures
R585	T1297	T1296	amod	functional,production
R5850	T8902	T8893	conj	procedures,cryosections
R5851	T8903	T8890	auxpass	were,carried
R5852	T8904	T8890	prt	out,carried
R5853	T8905	T8906	mark	as,described
R5854	T8906	T8890	advcl	described,carried
R5855	T8907	T8906	advmod	elsewhere,described
R5856	T8908	T8909	punct	[,19
R5857	T8909	T8906	parataxis	19,described
R5858	T8910	T8909	punct	],19
R5859	T8911	T8890	punct	.,carried
R586	T1298	T1299	punct	[,7
R5860	T8913	T8914	nsubjpass	Brains,fixed
R5861	T8915	T8913	prep	from,Brains
R5862	T8916	T8917	quantmod	3,5
R5863	T8917	T8920	nummod	5,mo
R5864	T8918	T8917	punct	-,5
R5865	T8919	T8917	quantmod	to,5
R5866	T8920	T8922	npadvmod	mo,old
R5867	T8921	T8920	punct	-,mo
R5868	T8922	T8924	amod	old,mice
R5869	T8923	T8922	punct	-,old
R587	T1299	T1294	parataxis	7,enhances
R5870	T8924	T8915	pobj	mice,from
R5871	T8925	T8924	nmod	C57Bl,mice
R5872	T8926	T8925	punct	/,C57Bl
R5873	T8927	T8925	nummod	6,C57Bl
R5874	T8928	T8914	auxpass	were,fixed
R5875	T8929	T8914	advmod	overnight,fixed
R5876	T8930	T8914	prep	in,fixed
R5877	T8931	T8932	nummod	2,%
R5878	T8932	T8933	compound	%,paraformaldehyde
R5879	T8933	T8930	pobj	paraformaldehyde,in
R588	T1300	T1299	nummod	6,7
R5880	T8934	T8914	punct	", ",fixed
R5881	T8935	T8914	conj	infused,fixed
R5882	T8936	T8935	prep	with,infused
R5883	T8937	T8938	nummod	30,%
R5884	T8938	T8939	compound	%,sucrose
R5885	T8939	T8936	pobj	sucrose,with
R5886	T8940	T8935	punct	", ",infused
R5887	T8941	T8935	cc	and,infused
R5888	T8942	T8935	conj	processed,infused
R5889	T8943	T8942	prep	for,processed
R589	T1301	T1299	punct	",",7
R5890	T8944	T8943	pobj	cryosectioning,for
R5891	T8945	T8914	punct	.,fixed
R5892	T8947	T8948	amod	Primary,neurons
R5893	T8948	T8950	nsubjpass	neurons,prepared
R5894	T8949	T8948	compound	brain,neurons
R5895	T8951	T8948	cc	and,neurons
R5896	T8952	T8953	amod	glial,cells
R5897	T8953	T8948	conj	cells,neurons
R5898	T8954	T8950	auxpass	were,prepared
R5899	T8955	T8950	prep	from,prepared
R59	T294	T293	pobj	RanBP2,with
R590	T1302	T1299	punct	],7
R5900	T8956	T8957	det	the,cortex
R5901	T8957	T8955	pobj	cortex,from
R5902	T8958	T8957	amod	cerebral,cortex
R5903	T8959	T8950	punct	.,prepared
R5904	T8961	T8962	nsubjpass	This,macerated
R5905	T8963	T8962	auxpass	was,macerated
R5906	T8964	T8962	prep	in,macerated
R5907	T8965	T8966	det	the,solution
R5908	T8966	T8964	pobj	solution,in
R5909	T8967	T8968	poss	Hank,Salt
R591	T1303	T1253	punct	", ",associates
R5910	T8968	T8966	compound	Salt,solution
R5911	T8969	T8967	case	's,Hank
R5912	T8970	T8968	compound	Balanced,Salt
R5913	T8971	T8962	cc	and,macerated
R5914	T8972	T8962	conj	triturated,macerated
R5915	T8973	T8962	punct	.,macerated
R5916	T8975	T8976	amod	Primary,cells
R5917	T8976	T8977	nsubjpass	cells,cultured
R5918	T8978	T8977	auxpass	were,cultured
R5919	T8979	T8977	prep	in,cultured
R592	T1304	T1305	mark	while,occurs
R5920	T8980	T8979	pobj	DMEM,in
R5921	T8981	T8982	punct	(,GIBCO
R5922	T8982	T8980	parataxis	GIBCO,DMEM
R5923	T8983	T8982	punct	", ",GIBCO
R5924	T8984	T8985	compound	San,Diego
R5925	T8985	T8982	npadvmod	Diego,GIBCO
R5926	T8986	T8982	punct	", ",GIBCO
R5927	T8987	T8982	npadvmod	California,GIBCO
R5928	T8988	T8982	punct	", ",GIBCO
R5929	T8989	T8990	compound	United,States
R593	T1305	T1253	advcl	occurs,associates
R5930	T8990	T8982	npadvmod	States,GIBCO
R5931	T8991	T8982	punct	),GIBCO
R5932	T8992	T8980	cc	and,DMEM
R5933	T8993	T8994	npadvmod	collagen,coated
R5934	T8994	T8996	amod	coated,dishes
R5935	T8995	T8994	punct	-,coated
R5936	T8996	T8980	conj	dishes,DMEM
R5937	T8997	T8998	nummod	35,mm
R5938	T8998	T8996	compound	mm,dishes
R5939	T8999	T8998	punct	-,mm
R594	T1306	T1307	det	the,interaction
R5940	T9000	T9001	compound	glass,bottom
R5941	T9001	T8996	compound	bottom,dishes
R5942	T9002	T8996	compound	culture,dishes
R5943	T9003	T9004	punct	(,Corporation
R5944	T9004	T8996	parataxis	Corporation,dishes
R5945	T9005	T9004	compound	MatTek,Corporation
R5946	T9006	T9004	punct	", ",Corporation
R5947	T9007	T9004	npadvmod	Ashland,Corporation
R5948	T9008	T9004	punct	", ",Corporation
R5949	T9009	T9004	npadvmod	Maine,Corporation
R595	T1307	T1305	nsubj	interaction,occurs
R5950	T9010	T9004	punct	),Corporation
R5951	T9011	T8977	prep	at,cultured
R5952	T9012	T9013	nummod	5,%
R5953	T9013	T9014	compound	%,CO2
R5954	T9014	T9011	pobj	CO2,at
R5955	T9015	T9014	punct	/,CO2
R5956	T9016	T9017	nummod	37,°C
R5957	T9017	T9014	appos	°C,CO2
R5958	T9018	T8977	prep	for,cultured
R5959	T9019	T9020	punct	~,2
R596	T1308	T1307	prep	of,interaction
R5960	T9020	T9021	nummod	2,d
R5961	T9021	T9018	pobj	d,for
R5962	T9022	T8977	cc	and,cultured
R5963	T9023	T8977	conj	processed,cultured
R5964	T9024	T9023	prep	for,processed
R5965	T9025	T9024	pobj	immunocytochemistry,for
R5966	T9026	T8977	punct	.,cultured
R5967	T9028	T9029	amod	Primary,antibodies
R5968	T9029	T9030	nsubjpass	antibodies,used
R5969	T9031	T9030	auxpass	were,used
R597	T1309	T1310	det	the,KBD
R5970	T9032	T9030	prep	at,used
R5971	T9033	T9032	pobj	concentrations,at
R5972	T9034	T9035	punct	~,10
R5973	T9035	T9038	nummod	10,μg
R5974	T9036	T9035	quantmod	2.5,10
R5975	T9037	T9035	punct	–,10
R5976	T9038	T9033	npadvmod	μg,concentrations
R5977	T9039	T9040	punct	/,ml
R5978	T9040	T9038	prep	ml,μg
R5979	T9041	T9030	punct	.,used
R598	T1310	T1308	pobj	KBD,of
R5980	T9043	T9044	npadvmod	Alexa,conjugated
R5981	T9044	T9051	amod	conjugated,antibodies
R5982	T9045	T9043	nummod	488,Alexa
R5983	T9046	T9043	punct	-,Alexa
R5984	T9047	T9043	cc	and,Alexa
R5985	T9048	T9043	conj	Alexa,Alexa
R5986	T9049	T9048	amod	594,Alexa
R5987	T9050	T9044	punct	-,conjugated
R5988	T9051	T9053	nsubjpass	antibodies,used
R5989	T9052	T9051	amod	secondary,antibodies
R599	T1311	T1310	prep	of,KBD
R5990	T9054	T9055	punct	(,μg
R5991	T9055	T9051	parataxis	μg,antibodies
R5992	T9056	T9055	nummod	2.5,μg
R5993	T9057	T9058	punct	/,ml
R5994	T9058	T9055	prep	ml,μg
R5995	T9059	T9055	punct	),μg
R5996	T9060	T9061	punct	(,Probes
R5997	T9061	T9051	parataxis	Probes,antibodies
R5998	T9062	T9061	compound	Molecular,Probes
R5999	T9063	T9061	punct	),Probes
R6	T235	T232	dobj	Activities,Modulates
R60	T295	T289	cc	and,significance
R600	T1312	T1311	pobj	RanBP2,of
R6000	T9064	T9053	auxpass	were,used
R6001	T9065	T9053	prep	for,used
R6002	T9066	T9065	pobj	visualization,for
R6003	T9067	T9066	prep	of,visualization
R6004	T9068	T9067	pobj	proteins,of
R6005	T9069	T9053	punct	.,used
R6006	T9071	T9072	nsubjpass	Crossover,found
R6007	T9073	T9071	prep	of,Crossover
R6008	T9074	T9075	amod	fluorescent,probes
R6009	T9075	T9073	pobj	probes,of
R601	T1313	T1307	prep	with,interaction
R6010	T9076	T9075	punct	", ",probes
R6011	T9077	T9075	conj	background,probes
R6012	T9078	T9077	punct	", ",background
R6013	T9079	T9077	cc	and,background
R6014	T9080	T9077	conj	autofluorescence,background
R6015	T9081	T9072	auxpass	were,found
R6016	T9082	T9083	aux	to,be
R6017	T9083	T9072	xcomp	be,found
R6018	T9084	T9083	acomp	negligible,be
R6019	T9085	T9072	punct	.,found
R602	T1314	T1315	det	a,subset
R6020	T9087	T9088	nsubjpass	Visualization,carried
R6021	T9089	T9087	prep	of,Visualization
R6022	T9090	T9089	pobj	specimens,of
R6023	T9091	T9087	cc	and,Visualization
R6024	T9092	T9087	conj	localization,Visualization
R6025	T9093	T9092	prep	of,localization
R6026	T9094	T9093	pobj	proteins,of
R6027	T9095	T9088	auxpass	were,carried
R6028	T9096	T9088	prt	out,carried
R6029	T9097	T9088	prep	by,carried
R603	T1315	T1313	pobj	subset,with
R6030	T9098	T9099	amod	wide,field
R6031	T9099	T9101	compound	field,microscopy
R6032	T9100	T9099	punct	-,field
R6033	T9101	T9097	pobj	microscopy,by
R6034	T9102	T9101	compound	epifluorescence,microscopy
R6035	T9103	T9088	prep	on,carried
R6036	T9104	T9105	nmod	Nikon,upright
R6037	T9105	T9103	pobj	upright,on
R6038	T9106	T9107	punct	(,Tokyo
R6039	T9107	T9104	parataxis	Tokyo,Nikon
R604	T1316	T1315	prep	of,subset
R6040	T9108	T9107	punct	", ",Tokyo
R6041	T9109	T9107	npadvmod	Japan,Tokyo
R6042	T9110	T9107	punct	),Tokyo
R6043	T9111	T9105	compound	E600,upright
R6044	T9112	T9105	cc	and,upright
R6045	T9113	T9114	nmod	TE2000U,microscopes
R6046	T9114	T9105	conj	microscopes,upright
R6047	T9115	T9113	amod	inverted,TE2000U
R6048	T9116	T9114	compound	research,microscopes
R6049	T9117	T9105	acl	equipped,upright
R605	T1317	T1318	det	the,proteins
R6050	T9118	T9117	prep	with,equipped
R6051	T9119	T9120	amod	similar,objectives
R6052	T9120	T9118	pobj	objectives,with
R6053	T9121	T9120	compound	Apochromat,objectives
R6054	T9122	T9088	punct	.,carried
R6055	T9124	T9125	nsubjpass	Images,acquired
R6056	T9126	T9124	prep	with,Images
R6057	T9127	T9128	det	the,E600
R6058	T9128	T9126	pobj	E600,with
R6059	T9129	T9128	compound	Nikon,E600
R606	T1318	T1316	pobj	proteins,of
R6060	T9130	T9125	auxpass	were,acquired
R6061	T9131	T9125	prep	with,acquired
R6062	T9132	T9133	det	a,camera
R6063	T9133	T9131	pobj	camera,with
R6064	T9134	T9135	nmod	SPOT,RT
R6065	T9135	T9133	nmod	RT,camera
R6066	T9136	T9135	punct	-,RT
R6067	T9137	T9133	amod	digital,camera
R6068	T9138	T9133	acl	coupled,camera
R6069	T9139	T9138	prep	to,coupled
R607	T1319	T1318	amod	conventional,proteins
R6070	T9140	T9141	det	the,microscope
R6071	T9141	T9139	pobj	microscope,to
R6072	T9142	T9138	cc	and,coupled
R6073	T9143	T9138	conj	driven,coupled
R6074	T9144	T9143	agent	by,driven
R6075	T9145	T9146	compound	SPOT,v4.0
R6076	T9146	T9148	compound	v4.0,software
R6077	T9147	T9146	compound	Imaging,v4.0
R6078	T9148	T9144	pobj	software,by
R6079	T9149	T9150	punct	(,Instruments
R608	T1320	T1321	npadvmod	microtubule,based
R6080	T9150	T9148	parataxis	Instruments,software
R6081	T9151	T9150	compound	Diagnostic,Instruments
R6082	T9152	T9150	punct	", ",Instruments
R6083	T9153	T9154	compound	Sterling,Heights
R6084	T9154	T9150	npadvmod	Heights,Instruments
R6085	T9155	T9150	punct	", ",Instruments
R6086	T9156	T9150	npadvmod	Michigan,Instruments
R6087	T9157	T9150	punct	", ",Instruments
R6088	T9158	T9159	compound	United,States
R6089	T9159	T9150	npadvmod	States,Instruments
R609	T1321	T1318	amod	based,proteins
R6090	T9160	T9150	punct	),Instruments
R6091	T9161	T9125	punct	.,acquired
R6092	T9163	T9164	det	All,images
R6093	T9164	T9165	nsubjpass	images,captured
R6094	T9166	T9165	auxpass	were,captured
R6095	T9167	T9165	prep	at,captured
R6096	T9168	T9169	compound	nonsaturating,levels
R6097	T9169	T9167	pobj	levels,at
R6098	T9170	T9169	compound	integration,levels
R6099	T9171	T9169	punct	", ",levels
R61	T296	T297	det	the,role
R610	T1322	T1321	punct	-,based
R6100	T9172	T9173	nummod	12,bit
R6101	T9173	T9175	nmod	bit,white
R6102	T9174	T9173	punct	-,bit
R6103	T9175	T9169	appos	white,levels
R6104	T9176	T9175	nmod	mono,white
R6105	T9177	T9175	amod	black,white
R6106	T9178	T9175	punct	/,white
R6107	T9179	T9165	punct	", ",captured
R6108	T9180	T9165	cc	and,captured
R6109	T9181	T9182	advmod	then,pseudo-colored
R611	T1323	T1318	compound	motor,proteins
R6110	T9182	T9165	conj	pseudo-colored,captured
R6111	T9183	T9165	punct	.,captured
R6112	T9185	T9186	compound	Protein,colocalization
R6113	T9186	T9187	compound	colocalization,analysis
R6114	T9187	T9188	nsubjpass	analysis,carried
R6115	T9189	T9188	auxpass	was,carried
R6116	T9190	T9188	prt	out,carried
R6117	T9191	T9188	prep	on,carried
R6118	T9192	T9193	det	the,microscope
R6119	T9193	T9191	pobj	microscope,on
R612	T1324	T1315	punct	", ",subset
R6120	T9194	T9195	compound	Nikon,TE2000U
R6121	T9195	T9193	compound	TE2000U,microscope
R6122	T9196	T9193	acl	equipped,microscope
R6123	T9197	T9196	prep	with,equipped
R6124	T9198	T9199	amod	appropriate,wheels
R6125	T9199	T9197	pobj	wheels,with
R6126	T9200	T9199	nmod	excitation,wheels
R6127	T9201	T9200	cc	and,excitation
R6128	T9202	T9200	conj	emission,excitation
R6129	T9203	T9199	compound	filter,wheels
R613	T1325	T1326	det	the,kinesins
R6130	T9204	T9199	punct	", ",wheels
R6131	T9205	T9206	compound	cube,filters
R6132	T9206	T9199	appos	filters,wheels
R6133	T9207	T9199	punct	", ",wheels
R6134	T9208	T9209	nummod	100,W
R6135	T9209	T9210	compound	W,source
R6136	T9210	T9199	appos	source,wheels
R6137	T9211	T9210	compound	mercury,source
R6138	T9212	T9210	compound	light,source
R6139	T9213	T9199	punct	", ",wheels
R614	T1326	T1315	appos	kinesins,subset
R6140	T9214	T9215	nmod	Nomarski,DIC
R6141	T9215	T9217	nmod	DIC,optics
R6142	T9216	T9215	punct	/,DIC
R6143	T9217	T9199	appos	optics,wheels
R6144	T9218	T9215	punct	", ",DIC
R6145	T9219	T9215	cc	and,DIC
R6146	T9220	T9221	compound	Plan,Apochromat
R6147	T9221	T9215	conj	Apochromat,DIC
R6148	T9222	T9217	punct	(,optics
R6149	T9223	T9224	nummod	100,objectives
R615	T1327	T1326	punct	", ",kinesins
R6150	T9224	T9217	appos	objectives,optics
R6151	T9225	T9223	punct	×,100
R6152	T9226	T9223	punct	", ",100
R6153	T9227	T9223	conj	60,100
R6154	T9228	T9227	punct	×,60
R6155	T9229	T9227	punct	", ",60
R6156	T9230	T9227	cc	and,60
R6157	T9231	T9227	conj	40,60
R6158	T9232	T9231	punct	×,40
R6159	T9233	T9224	compound	oil,objectives
R616	T1328	T1326	appos	KIF5B,kinesins
R6160	T9234	T9224	prep	with,objectives
R6161	T9235	T9234	pobj	NA,with
R6162	T9236	T9235	prep	of,NA
R6163	T9237	T9236	pobj	1.4,of
R6164	T9238	T9224	punct	", ",objectives
R6165	T9239	T9240	nummod	40,LWD
R6166	T9240	T9242	nmod	LWD,objectives
R6167	T9241	T9239	punct	×,40
R6168	T9242	T9224	conj	objectives,objectives
R6169	T9243	T9240	punct	", ",LWD
R617	T1329	T1328	cc	and,KIF5B
R6170	T9244	T9240	cc	and,LWD
R6171	T9245	T9246	nummod	20,LWD
R6172	T9246	T9240	conj	LWD,LWD
R6173	T9247	T9245	punct	×,20
R6174	T9248	T9242	cc	and,objectives
R6175	T9249	T9250	amod	encoded,Stage
R6176	T9250	T9242	conj	Stage,objectives
R6177	T9251	T9250	amod	motorized,Stage
R6178	T9252	T9250	compound	Z,Stage
R6179	T9253	T9250	punct	-,Stage
R618	T1330	T1328	conj	KIF5C,KIF5B
R6180	T9254	T9255	punct	(,Scientific
R6181	T9255	T9250	parataxis	Scientific,Stage
R6182	T9256	T9255	compound	Prior,Scientific
R6183	T9257	T9255	punct	", ",Scientific
R6184	T9258	T9255	npadvmod	Rockland,Scientific
R6185	T9259	T9255	punct	", ",Scientific
R6186	T9260	T9255	npadvmod	Maryland,Scientific
R6187	T9261	T9255	punct	", ",Scientific
R6188	T9262	T9263	compound	United,States
R6189	T9263	T9255	npadvmod	States,Scientific
R619	T1331	T1305	punct	", ",occurs
R6190	T9264	T9255	punct	),Scientific
R6191	T9265	T9188	punct	.,carried
R6192	T9267	T9268	nsubjpass	Images,obtained
R6193	T9269	T9267	prep	with,Images
R6194	T9270	T9271	det	the,microscope
R6195	T9271	T9269	pobj	microscope,with
R6196	T9272	T9271	amod	inverted,microscope
R6197	T9273	T9274	compound	Nikon,TE2000U
R6198	T9274	T9271	compound	TE2000U,microscope
R6199	T9275	T9268	auxpass	were,obtained
R62	T297	T289	conj	role,significance
R620	T1332	T1305	advmod	selectively,occurs
R6200	T9276	T9268	advcl	using,obtained
R6201	T9277	T9278	det	a,camera
R6202	T9278	T9276	dobj	camera,using
R6203	T9279	T9278	compound	CCD,camera
R6204	T9280	T9278	punct	(,camera
R6205	T9281	T9282	compound	CoolSNAP,HQ
R6206	T9282	T9278	appos	HQ,camera
R6207	T9283	T9284	punct	;,Scientific
R6208	T9284	T9278	parataxis	Scientific,camera
R6209	T9285	T9284	compound	Roper,Scientific
R621	T1333	T1305	prep	in,occurs
R6210	T9286	T9284	punct	", ",Scientific
R6211	T9287	T9284	npadvmod	Trenton,Scientific
R6212	T9288	T9284	punct	", ",Scientific
R6213	T9289	T9290	compound	New,Jersey
R6214	T9290	T9284	npadvmod	Jersey,Scientific
R6215	T9291	T9284	punct	", ",Scientific
R6216	T9292	T9293	compound	United,States
R6217	T9293	T9284	npadvmod	States,Scientific
R6218	T9294	T9284	punct	),Scientific
R6219	T9295	T9268	punct	.,obtained
R622	T1334	T1335	det	the,system
R6220	T9297	T9298	nsubjpass	Images,analyzed
R6221	T9299	T9297	acl	acquired,Images
R6222	T9300	T9299	prep	under,acquired
R6223	T9301	T9302	amod	identical,parameters
R6224	T9302	T9300	pobj	parameters,under
R6225	T9303	T9302	compound	acquisition,parameters
R6226	T9304	T9298	auxpass	were,analyzed
R6227	T9305	T9298	advcl	using,analyzed
R6228	T9306	T9307	compound	Metamorph,v6.2
R6229	T9307	T9305	dobj	v6.2,using
R623	T1335	T1333	pobj	system,in
R6230	T9308	T9307	compound	Software,v6.2
R6231	T9309	T9310	punct	(,Imaging
R6232	T9310	T9307	parataxis	Imaging,v6.2
R6233	T9311	T9310	compound	Universal,Imaging
R6234	T9312	T9310	punct	", ",Imaging
R6235	T9313	T9310	npadvmod	Glendale,Imaging
R6236	T9314	T9310	punct	", ",Imaging
R6237	T9315	T9310	npadvmod	Wisconsin,Imaging
R6238	T9316	T9310	punct	", ",Imaging
R6239	T9317	T9318	compound	United,States
R624	T1336	T1335	amod	central,system
R6240	T9318	T9310	npadvmod	States,Imaging
R6241	T9319	T9310	punct	),Imaging
R6242	T9320	T9298	punct	.,analyzed
R6243	T9322	T9323	advmod	Whenever,applicable
R6244	T9323	T9324	advcl	applicable,captured
R6245	T9325	T9324	punct	", ",captured
R6246	T9326	T9327	amod	serial,stacks
R6247	T9327	T9324	nsubjpass	stacks,captured
R6248	T9328	T9327	amod	optical,stacks
R6249	T9329	T9327	compound	Z,stacks
R625	T1337	T1335	amod	nervous,system
R6250	T9330	T9327	punct	-,stacks
R6251	T9331	T9327	punct	(,stacks
R6252	T9332	T9333	quantmod	20,30
R6253	T9333	T9335	nummod	30,planes
R6254	T9334	T9333	punct	–,30
R6255	T9335	T9327	appos	planes,stacks
R6256	T9336	T9335	amod	focal,planes
R6257	T9337	T9335	prep	at,planes
R6258	T9338	T9339	nummod	100,nm
R6259	T9339	T9341	compound	nm,intervals
R626	T1338	T1335	punct	(,system
R6260	T9340	T9339	punct	-,nm
R6261	T9341	T9337	pobj	intervals,at
R6262	T9342	T9324	punct	),captured
R6263	T9343	T9324	auxpass	were,captured
R6264	T9344	T9324	cc	and,captured
R6265	T9345	T9346	advmod	computationally,processed
R6266	T9346	T9324	conj	processed,captured
R6267	T9347	T9346	prep	by,processed
R6268	T9348	T9349	nummod	3,D
R6269	T9349	T9351	nmod	D,methods
R627	T1339	T1335	appos	CNS,system
R6270	T9350	T9349	punct	-,D
R6271	T9351	T9347	pobj	methods,by
R6272	T9352	T9351	amod	blind,methods
R6273	T9353	T9351	compound	deconvolution,methods
R6274	T9354	T9346	prep	with,processed
R6275	T9355	T9356	det	the,software
R6276	T9356	T9354	pobj	software,with
R6277	T9357	T9356	amod	same,software
R6278	T9358	T9324	punct	.,captured
R6279	T9492	T9491	prep	of,Generation
R628	T1340	T1305	punct	),occurs
R6280	T9493	T9494	det	a,line
R6281	T9494	T9492	pobj	line,of
R6282	T9495	T9494	compound	mouse,line
R6283	T9496	T9494	prep	with,line
R6284	T9497	T9498	det	a,locus
R6285	T9498	T9496	pobj	locus,with
R6286	T9499	T9498	amod	disrupted,locus
R6287	T9500	T9498	compound	RanBP2,locus
R6288	T9501	T9491	cc	and,Generation
R6289	T9502	T9503	amod	histological,analysis
R629	T1341	T1342	punct	[,13
R6290	T9503	T9491	conj	analysis,Generation
R6291	T9504	T9503	prep	of,analysis
R6292	T9505	T9506	amod	trapped,mice
R6293	T9506	T9504	pobj	mice,of
R6294	T9507	T9506	compound	RanBP2,mice
R6295	T9508	T9503	punct	.,analysis
R6296	T9510	T9511	det	A,line
R6297	T9511	T9518	nsubjpass	line,obtained
R6298	T9512	T9513	npadvmod	feeder,independent
R6299	T9513	T9511	amod	independent,line
R63	T298	T297	amod	genetic,role
R630	T1342	T1305	parataxis	13,occurs
R6300	T9514	T9513	punct	-,independent
R6301	T9515	T9511	compound	mouse,line
R6302	T9516	T9517	compound	ES,cell
R6303	T9517	T9511	compound	cell,line
R6304	T9519	T9511	acl	derived,line
R6305	T9520	T9519	prep	from,derived
R6306	T9521	T9522	det	the,strain
R6307	T9522	T9520	pobj	strain,from
R6308	T9523	T9524	compound	129P2,OlaHsd
R6309	T9524	T9522	compound	OlaHsd,strain
R631	T1343	T1342	punct	],13
R6310	T9525	T9524	punct	/,OlaHsd
R6311	T9526	T9519	cc	and,derived
R6312	T9527	T9519	conj	harboring,derived
R6313	T9528	T9529	det	a,locus
R6314	T9529	T9527	dobj	locus,harboring
R6315	T9530	T9529	amod	disrupted,locus
R6316	T9531	T9529	compound	RanBP2,locus
R6317	T9532	T9529	prep	by,locus
R6318	T9533	T9534	compound	insertion,mutagenesis
R6319	T9534	T9532	pobj	mutagenesis,by
R632	T1344	T1253	punct	.,associates
R6320	T9535	T9534	prep	with,mutagenesis
R6321	T9536	T9537	det	the,vector
R6322	T9537	T9535	pobj	vector,with
R6323	T9538	T9537	compound	gene,vector
R6324	T9539	T9537	compound	trap,vector
R6325	T9540	T9537	punct	", ",vector
R6326	T9541	T9537	appos	pGT0pfs,vector
R6327	T9542	T9543	punct	[,68
R6328	T9543	T9541	parataxis	68,pGT0pfs
R6329	T9544	T9543	nummod	67,68
R633	T1346	T1347	det	A,set
R6330	T9545	T9543	punct	",",68
R6331	T9546	T9543	punct	],68
R6332	T9547	T9537	punct	(,vector
R6333	T9548	T9537	acl	generated,vector
R6334	T9549	T9548	agent	by,generated
R6335	T9550	T9551	compound	W.,Skarnes
R6336	T9551	T9549	pobj	Skarnes,by
R6337	T9552	T9518	punct	),obtained
R6338	T9553	T9518	punct	", ",obtained
R6339	T9554	T9518	auxpass	was,obtained
R634	T1347	T1349	nsubj	set,are
R6340	T9555	T9518	prep	from,obtained
R6341	T9556	T9557	det	the,Center
R6342	T9557	T9555	pobj	Center,from
R6343	T9558	T9559	compound	Mutant,Mouse
R6344	T9559	T9557	compound	Mouse,Center
R6345	T9560	T9557	compound	Regional,Center
R6346	T9561	T9557	compound	Resource,Center
R6347	T9562	T9563	punct	(,University
R6348	T9563	T9557	parataxis	University,Center
R6349	T9564	T9563	prep	of,University
R635	T1348	T1347	amod	diverse,set
R6350	T9565	T9566	compound	California,Davis
R6351	T9566	T9564	pobj	Davis,of
R6352	T9567	T9563	punct	", ",University
R6353	T9568	T9563	npadvmod	Davis,University
R6354	T9569	T9563	punct	", ",University
R6355	T9570	T9563	npadvmod	California,University
R6356	T9571	T9563	punct	", ",University
R6357	T9572	T9573	compound	United,States
R6358	T9573	T9563	npadvmod	States,University
R6359	T9574	T9563	punct	),University
R636	T1350	T1347	prep	of,set
R6360	T9575	T9518	punct	.,obtained
R6361	T9577	T9578	det	The,vector
R6362	T9578	T9581	nsubj	vector,contains
R6363	T9579	T9580	compound	gene,trap
R6364	T9580	T9578	compound	trap,vector
R6365	T9582	T9583	det	a,gene
R6366	T9583	T9581	dobj	gene,contains
R6367	T9584	T9583	amod	promoterless,gene
R6368	T9585	T9583	compound	neo-lacZ,gene
R6369	T9586	T9583	compound	fusion,gene
R637	T1351	T1352	amod	additional,partners
R6370	T9587	T9583	prep	with,gene
R6371	T9588	T9589	det	a,site
R6372	T9589	T9587	pobj	site,with
R6373	T9590	T9589	compound	splice,site
R6374	T9591	T9589	compound	acceptor,site
R6375	T9592	T9589	prep	at,site
R6376	T9593	T9594	det	the,end
R6377	T9594	T9592	pobj	end,at
R6378	T9595	T9594	nummod	5,end
R6379	T9596	T9595	punct	′,5
R638	T1352	T1350	pobj	partners,of
R6380	T9597	T9589	acl	followed,site
R6381	T9598	T9597	agent	by,followed
R6382	T9599	T9600	det	an,site
R6383	T9600	T9598	pobj	site,by
R6384	T9601	T9600	amod	internal,site
R6385	T9602	T9600	compound	ribosome,site
R6386	T9603	T9600	compound	entry,site
R6387	T9604	T9600	punct	(,site
R6388	T9605	T9600	appos	IRES,site
R6389	T9606	T9600	punct	),site
R639	T1353	T1352	amod	molecular,partners
R6390	T9607	T9600	cc	and,site
R6391	T9608	T9609	det	the,cassette
R6392	T9609	T9600	conj	cassette,site
R6393	T9610	T9609	amod	human,cassette
R6394	T9611	T9612	amod	placental,phosphatase
R6395	T9612	T9609	nmod	phosphatase,cassette
R6396	T9613	T9612	nmod	alkaline,phosphatase
R6397	T9614	T9612	punct	(,phosphatase
R6398	T9615	T9612	appos	PLAP,phosphatase
R6399	T9616	T9609	punct	),cassette
R64	T299	T298	cc	and,genetic
R640	T1354	T1347	punct	", ",set
R6400	T9617	T9609	compound	reporter,cassette
R6401	T9618	T9581	punct	.,contains
R6402	T9620	T9621	det	The,line
R6403	T9621	T9625	nsubjpass	line,injected
R6404	T9622	T9623	compound	RanBP2,129Ola
R6405	T9623	T9621	compound	129Ola,line
R6406	T9624	T9621	compound	ES,line
R6407	T9626	T9625	auxpass	was,injected
R6408	T9627	T9625	prep	into,injected
R6409	T9628	T9629	compound	C57BL,6J
R641	T1355	T1347	appos	each,set
R6410	T9629	T9631	compound	6J,blastocysts
R6411	T9630	T9629	punct	/,6J
R6412	T9631	T9627	pobj	blastocysts,into
R6413	T9632	T9625	cc	and,injected
R6414	T9633	T9634	nummod	four,chimeras
R6415	T9634	T9636	nsubjpass	chimeras,generated
R6416	T9635	T9634	amod	male,chimeras
R6417	T9636	T9625	conj	generated,injected
R6418	T9637	T9636	auxpass	were,generated
R6419	T9638	T9636	punct	.,generated
R642	T1356	T1355	acl	associating,each
R6420	T9640	T9641	nsubjpass	These,bred
R6421	T9642	T9641	auxpass	were,bred
R6422	T9643	T9641	prep	into,bred
R6423	T9644	T9645	nmod	129P2,OlaHsd
R6424	T9645	T9647	nmod	OlaHsd,females
R6425	T9646	T9645	punct	/,OlaHsd
R6426	T9647	T9643	pobj	females,into
R6427	T9648	T9649	punct	(,Harlan
R6428	T9649	T9645	parataxis	Harlan,OlaHsd
R6429	T9650	T9649	punct	", ",Harlan
R643	T1357	T1356	advmod	specifically,associating
R6430	T9651	T9649	npadvmod	Indianapolis,Harlan
R6431	T9652	T9649	punct	", ",Harlan
R6432	T9653	T9649	npadvmod	Indiana,Harlan
R6433	T9654	T9649	punct	", ",Harlan
R6434	T9655	T9656	compound	United,States
R6435	T9656	T9649	npadvmod	States,Harlan
R6436	T9657	T9649	punct	),Harlan
R6437	T9658	T9645	cc	and,OlaHsd
R6438	T9659	T9660	compound	C57BL,6J
R6439	T9660	T9645	conj	6J,OlaHsd
R644	T1358	T1356	prep	with,associating
R6440	T9661	T9660	punct	/,6J
R6441	T9662	T9663	punct	(,Laboratory
R6442	T9663	T9660	parataxis	Laboratory,6J
R6443	T9664	T9663	compound	Jackson,Laboratory
R6444	T9665	T9663	punct	", ",Laboratory
R6445	T9666	T9667	compound	Bar,Harbor
R6446	T9667	T9663	npadvmod	Harbor,Laboratory
R6447	T9668	T9663	punct	", ",Laboratory
R6448	T9669	T9663	npadvmod	Maine,Laboratory
R6449	T9670	T9663	punct	", ",Laboratory
R645	T1359	T1360	det	a,domain
R6450	T9671	T9672	compound	United,States
R6451	T9672	T9663	npadvmod	States,Laboratory
R6452	T9673	T9663	punct	),Laboratory
R6453	T9674	T9641	punct	.,bred
R6454	T9676	T9677	det	The,lines
R6455	T9677	T9683	nsubjpass	lines,tested
R6456	T9678	T9677	nummod	two,lines
R6457	T9679	T9677	amod	resulting,lines
R6458	T9680	T9679	cc	and,resulting
R6459	T9681	T9679	conj	independent,resulting
R646	T1360	T1358	pobj	domain,with
R6460	T9682	T9677	compound	F1s,lines
R6461	T9684	T9677	punct	", ",lines
R6462	T9685	T9677	prep	with,lines
R6463	T9686	T9687	compound	129P2,OlaHsd
R6464	T9687	T9685	pobj	OlaHsd,with
R6465	T9688	T9687	punct	/,OlaHsd
R6466	T9689	T9687	amod	inbred,OlaHsd
R6467	T9690	T9687	cc	and,OlaHsd
R6468	T9691	T9692	nmod	129P2,6J
R6469	T9692	T9698	nmod	6J,backgrounds
R647	T1361	T1360	amod	selective,domain
R6470	T9693	T9692	punct	/,6J
R6471	T9694	T9692	nmod	OlaHsd,6J
R6472	T9695	T9692	punct	/,6J
R6473	T9696	T9692	nmod	C57BL,6J
R6474	T9697	T9692	punct	/,6J
R6475	T9698	T9687	conj	backgrounds,OlaHsd
R6476	T9699	T9692	amod	mixed,6J
R6477	T9700	T9692	amod	genetic,6J
R6478	T9701	T9683	punct	", ",tested
R6479	T9702	T9683	auxpass	were,tested
R648	T1362	T1360	prep	of,domain
R6480	T9703	T9683	prep	for,tested
R6481	T9704	T9705	compound	germline,transmission
R6482	T9705	T9703	pobj	transmission,for
R6483	T9706	T9683	prep	by,tested
R6484	T9707	T9708	nmod	PCR,analysis
R6485	T9708	T9706	pobj	analysis,by
R6486	T9709	T9707	cc	and,PCR
R6487	T9710	T9711	compound	Southern,blot
R6488	T9711	T9707	conj	blot,PCR
R6489	T9712	T9708	prep	of,analysis
R649	T1363	T1362	pobj	RanBP2,of
R6490	T9713	T9714	nmod	tail,DNA
R6491	T9714	T9712	pobj	DNA,of
R6492	T9715	T9714	amod	genomic,DNA
R6493	T9716	T9683	punct	.,tested
R6494	T9718	T9719	nmod	β,gal
R6495	T9719	T9721	nmod	gal,activities
R6496	T9720	T9719	punct	-,gal
R6497	T9721	T9724	nsubjpass	activities,detected
R6498	T9722	T9719	cc	and,gal
R6499	T9723	T9719	conj	PLAP,gal
R65	T300	T298	conj	physiological,genetic
R650	T1364	T1349	punct	", ",are
R6500	T9725	T9721	prep	in,activities
R6501	T9726	T9727	amod	whole,mount
R6502	T9727	T9728	compound	mount,embryos
R6503	T9728	T9725	pobj	embryos,in
R6504	T9729	T9728	cc	and,embryos
R6505	T9730	T9731	amod	retinal,sections
R6506	T9731	T9728	conj	sections,embryos
R6507	T9732	T9724	auxpass	were,detected
R6508	T9733	T9724	punct	", ",detected
R6509	T9734	T9724	advmod	respectively,detected
R651	T1365	T1349	acomp	likely,are
R6510	T9735	T9724	punct	", ",detected
R6511	T9736	T9724	prep	by,detected
R6512	T9737	T9736	pobj	incubation,by
R6513	T9738	T9737	prep	with,incubation
R6514	T9739	T9740	nummod	5,bromo
R6515	T9740	T9742	nmod	bromo,galactopyranoside
R6516	T9741	T9740	punct	-,bromo
R6517	T9742	T9755	nmod	galactopyranoside,buffer
R6518	T9743	T9742	punct	-,galactopyranoside
R6519	T9744	T9742	nummod	4,galactopyranoside
R652	T1366	T1367	aux	to,impart
R6520	T9745	T9744	punct	-,4
R6521	T9746	T9742	nmod	chloro,galactopyranoside
R6522	T9747	T9742	punct	-,galactopyranoside
R6523	T9748	T9749	nummod	3,β
R6524	T9749	T9742	nmod	β,galactopyranoside
R6525	T9750	T9749	punct	-,β
R6526	T9751	T9749	nmod	indolyl,β
R6527	T9752	T9742	punct	-,galactopyranoside
R6528	T9753	T9742	nmod	D,galactopyranoside
R6529	T9754	T9742	punct	-,galactopyranoside
R653	T1367	T1365	xcomp	impart,likely
R6530	T9755	T9738	pobj	buffer,with
R6531	T9756	T9742	cc	and,galactopyranoside
R6532	T9757	T9742	conj	AP,galactopyranoside
R6533	T9758	T9755	compound	staining,buffer
R6534	T9759	T9760	punct	(,tetrazolium
R6535	T9760	T9737	parataxis	tetrazolium,incubation
R6536	T9761	T9762	nummod	0.1,mg
R6537	T9762	T9763	nmod	mg,phosphate
R6538	T9763	T9760	dep	phosphate,tetrazolium
R6539	T9764	T9765	punct	/,ml
R654	T1368	T1367	cc	and,impart
R6540	T9765	T9762	prep	ml,mg
R6541	T9766	T9767	nummod	5,bromo
R6542	T9767	T9763	nmod	bromo,phosphate
R6543	T9768	T9767	punct	-,bromo
R6544	T9769	T9763	punct	-,phosphate
R6545	T9770	T9771	nummod	4,chloro
R6546	T9771	T9763	nmod	chloro,phosphate
R6547	T9772	T9771	punct	-,chloro
R6548	T9773	T9763	punct	-,phosphate
R6549	T9774	T9763	nummod	3,phosphate
R655	T1369	T1367	conj	integrate,impart
R6550	T9775	T9763	punct	-,phosphate
R6551	T9776	T9763	compound	indolyl,phosphate
R6552	T9777	T9760	punct	", ",tetrazolium
R6553	T9778	T9779	nummod	1,mg
R6554	T9779	T9760	nmod	mg,tetrazolium
R6555	T9780	T9781	punct	/,ml
R6556	T9781	T9779	prep	ml,mg
R6557	T9782	T9760	amod	nitroblue,tetrazolium
R6558	T9783	T9760	prep	in,tetrazolium
R6559	T9784	T9785	nummod	100,mM
R656	T1370	T1371	amod	additional,roles
R6560	T9785	T9786	compound	mM,HCl
R6561	T9786	T9783	pobj	HCl,in
R6562	T9787	T9786	compound	Tris,HCl
R6563	T9788	T9786	punct	-,HCl
R6564	T9789	T9790	punct	(,pH
R6565	T9790	T9786	parataxis	pH,HCl
R6566	T9791	T9790	nummod	9.5,pH
R6567	T9792	T9790	punct	),pH
R6568	T9793	T9786	punct	", ",HCl
R6569	T9794	T9795	nummod	100,mM
R657	T1371	T1369	dobj	roles,integrate
R6570	T9795	T9796	compound	mM,NaCl
R6571	T9796	T9786	appos	NaCl,HCl
R6572	T9797	T9786	punct	", ",HCl
R6573	T9798	T9799	nummod	5,mM
R6574	T9799	T9800	compound	mM,MgCl2
R6575	T9800	T9786	appos	MgCl2,HCl
R6576	T9801	T9760	punct	),tetrazolium
R6577	T9802	T9803	mark	as,described
R6578	T9803	T9724	advcl	described,detected
R6579	T9804	T9803	advmod	elsewhere,described
R658	T1372	T1371	prep	to,roles
R6580	T9805	T9806	punct	[,68
R6581	T9806	T9803	parataxis	68,described
R6582	T9807	T9806	nummod	67,68
R6583	T9808	T9806	punct	",",68
R6584	T9809	T9806	punct	],68
R6585	T9810	T9724	punct	.,detected
R6586	T9862	T9863	compound	Western,blot
R6587	T9864	T9863	cc	and,blot
R6588	T9865	T9866	compound	quantitation,analysis
R6589	T9866	T9863	conj	analysis,blot
R659	T1373	T1372	pobj	RanBP2,to
R6590	T9867	T9863	prep	of,blot
R6591	T9868	T9869	compound	protein,expression
R6592	T9869	T9867	pobj	expression,of
R6593	T9870	T9869	cc	and,expression
R6594	T9871	T9872	compound	ATP,levels
R6595	T9872	T9869	conj	levels,expression
R6596	T9873	T9863	punct	.,blot
R6597	T9875	T9876	nsubjpass	Homogenates,prepared
R6598	T9877	T9875	cc	and,Homogenates
R6599	T9878	T9879	npadvmod	detergent,solubilized
R66	T301	T297	punct	(,role
R660	T1374	T1349	punct	.,are
R6600	T9879	T9880	amod	solubilized,extracts
R6601	T9880	T9875	conj	extracts,Homogenates
R6602	T9881	T9875	prep	of,Homogenates
R6603	T9882	T9881	pobj	tissues,of
R6604	T9883	T9882	cc	and,tissues
R6605	T9884	T9882	conj	immunoblots,tissues
R6606	T9885	T9876	auxpass	were,prepared
R6607	T9886	T9887	mark	as,described
R6608	T9887	T9876	advcl	described,prepared
R6609	T9888	T9887	advmod	elsewhere,described
R661	T1376	T1377	prep	For,associate
R6610	T9889	T9890	punct	[,69
R6611	T9890	T9887	parataxis	69,described
R6612	T9891	T9890	punct	],69
R6613	T9892	T9876	punct	.,prepared
R6614	T9894	T9895	nsubjpass	Quantitation,carried
R6615	T9896	T9894	prep	of,Quantitation
R6616	T9897	T9898	compound	immunoblot,bands
R6617	T9898	T9896	pobj	bands,of
R6618	T9899	T9894	acl	calibrated,Quantitation
R6619	T9900	T9899	prep	against,calibrated
R662	T1378	T1376	pobj	example,For
R6620	T9901	T9902	amod	internal,bands
R6621	T9902	T9900	pobj	bands,against
R6622	T9903	T9902	compound	marker,bands
R6623	T9904	T9902	punct	", ",bands
R6624	T9905	T9906	amod	such,as
R6625	T9906	T9902	prep	as,bands
R6626	T9907	T9906	pobj	mHsp70,as
R6627	T9908	T9907	cc	and,mHsp70
R6628	T9909	T9907	conj	Nup153,mHsp70
R6629	T9910	T9895	punct	", ",carried
R663	T1379	T1377	punct	", ",associate
R6630	T9911	T9895	auxpass	was,carried
R6631	T9912	T9895	prt	out,carried
R6632	T9913	T9895	prep	with,carried
R6633	T9914	T9915	compound	Gel,Analyzer
R6634	T9915	T9913	pobj	Analyzer,with
R6635	T9916	T9915	compound	Pro,Analyzer
R6636	T9917	T9918	punct	(,MediaCybernetics
R6637	T9918	T9915	parataxis	MediaCybernetics,Analyzer
R6638	T9919	T9918	punct	", ",MediaCybernetics
R6639	T9920	T9921	compound	San,Diego
R664	T1380	T1381	det	the,domain
R6640	T9921	T9918	npadvmod	Diego,MediaCybernetics
R6641	T9922	T9918	punct	", ",MediaCybernetics
R6642	T9923	T9918	npadvmod	California,MediaCybernetics
R6643	T9924	T9918	punct	", ",MediaCybernetics
R6644	T9925	T9926	compound	United,States
R6645	T9926	T9918	npadvmod	States,MediaCybernetics
R6646	T9927	T9918	punct	),MediaCybernetics
R6647	T9928	T9895	punct	.,carried
R6648	T9930	T9931	prep	For,homogenized
R6649	T9932	T9930	pobj	determination,For
R665	T1381	T1377	nsubj	domain,associate
R6650	T9933	T9932	prep	of,determination
R6651	T9934	T9935	compound	ATP,levels
R6652	T9935	T9933	pobj	levels,of
R6653	T9936	T9931	punct	", ",homogenized
R6654	T9937	T9938	advmod	freshly,dissected
R6655	T9938	T9939	amod	dissected,tissues
R6656	T9939	T9931	nsubjpass	tissues,homogenized
R6657	T9940	T9938	cc	and,dissected
R6658	T9941	T9942	npadvmod	flash,frozen
R6659	T9942	T9938	conj	frozen,dissected
R666	T1382	T1383	npadvmod	cyclophilin,like
R6660	T9943	T9942	punct	-,frozen
R6661	T9944	T9931	auxpass	were,homogenized
R6662	T9945	T9931	prep	in,homogenized
R6663	T9946	T9947	nummod	2.5,%
R6664	T9947	T9945	pobj	%,in
R6665	T9948	T9949	amod	trichloroacetic,acid
R6666	T9949	T9947	appos	acid,%
R6667	T9950	T9949	punct	(,acid
R6668	T9951	T9949	appos	TCA,acid
R6669	T9952	T9931	punct	),homogenized
R667	T1383	T1381	amod	like,domain
R6670	T9953	T9931	punct	", ",homogenized
R6671	T9954	T9931	conj	neutralized,homogenized
R6672	T9955	T9954	punct	", ",neutralized
R6673	T9956	T9954	cc	and,neutralized
R6674	T9957	T9954	conj	diluted,neutralized
R6675	T9958	T9957	prep	to,diluted
R6676	T9959	T9960	det	a,concentration
R6677	T9960	T9958	pobj	concentration,to
R6678	T9961	T9960	amod	final,concentration
R6679	T9962	T9960	prep	of,concentration
R668	T1384	T1383	punct	-,like
R6680	T9963	T9964	nummod	0.1,%
R6681	T9964	T9962	pobj	%,of
R6682	T9965	T9957	prep	with,diluted
R6683	T9966	T9967	compound	Tris,Acetate
R6684	T9967	T9965	pobj	Acetate,with
R6685	T9968	T9967	punct	-,Acetate
R6686	T9969	T9970	punct	(,pH
R6687	T9970	T9967	parataxis	pH,Acetate
R6688	T9971	T9970	nummod	7.75,pH
R6689	T9972	T9970	punct	),pH
R669	T1385	T1381	punct	(,domain
R6690	T9973	T9931	punct	.,homogenized
R6691	T9975	T9976	compound	ATP,measurements
R6692	T9976	T9977	nsubjpass	measurements,carried
R6693	T9978	T9977	auxpass	were,carried
R6694	T9979	T9977	prt	out,carried
R6695	T9980	T9977	prep	with,carried
R6696	T9981	T9982	det	the,Kit
R6697	T9982	T9980	pobj	Kit,with
R6698	T9983	T9982	compound	ENLITEN,Kit
R6699	T9984	T9985	compound	ATP,System
R67	T302	T297	nmod	s,role
R670	T1386	T1381	appos	CLD,domain
R6700	T9985	T9982	compound	System,Kit
R6701	T9986	T9985	compound	Assay,System
R6702	T9987	T9988	compound	Bioluminescence,Detection
R6703	T9988	T9982	compound	Detection,Kit
R6704	T9989	T9982	prep	for,Kit
R6705	T9990	T9989	pobj	ATP,for
R6706	T9991	T9992	punct	(,Promega
R6707	T9992	T9982	parataxis	Promega,Kit
R6708	T9993	T9992	punct	", ",Promega
R6709	T9994	T9992	npadvmod	Madison,Promega
R671	T1387	T1381	punct	),domain
R6710	T9995	T9992	punct	", ",Promega
R6711	T9996	T9992	npadvmod	Wisconsin,Promega
R6712	T9997	T9992	punct	", ",Promega
R6713	T9998	T9999	compound	United,States
R6714	T9999	T9992	npadvmod	States,Promega
R6715	T10000	T9992	punct	),Promega
R6716	T10001	T9977	prep	as,carried
R6717	T10002	T10001	prep	per,as
R6718	T10003	T10004	det	the,manufacturer
R6719	T10004	T10005	poss	manufacturer,instruction
R672	T1388	T1381	punct	", ",domain
R6720	T10005	T10002	pobj	instruction,per
R6721	T10006	T10004	case	's,manufacturer
R6722	T10007	T9977	prep	in,carried
R6723	T10008	T10009	compound	SpectraMax,M5
R6724	T10009	T10007	pobj	M5,in
R6725	T10010	T10009	punct	-,M5
R6726	T10011	T10012	punct	(,Devices
R6727	T10012	T10009	parataxis	Devices,M5
R6728	T10013	T10012	compound	Molecular,Devices
R6729	T10014	T10012	punct	", ",Devices
R673	T1389	T1390	det	the,domains
R6730	T10015	T10012	npadvmod	Sunnyvale,Devices
R6731	T10016	T10012	punct	", ",Devices
R6732	T10017	T10012	npadvmod	California,Devices
R6733	T10018	T10012	punct	", ",Devices
R6734	T10019	T10020	compound	United,States
R6735	T10020	T10012	npadvmod	States,Devices
R6736	T10021	T10012	punct	),Devices
R6737	T10022	T9977	punct	.,carried
R6738	T10024	T10025	compound	Protein,concentration
R6739	T10025	T10026	nsubjpass	concentration,measured
R674	T1390	T1381	appos	domains,domain
R6740	T10027	T10026	auxpass	was,measured
R6741	T10028	T10026	prep	by,measured
R6742	T10029	T10030	det	the,method
R6743	T10030	T10028	pobj	method,by
R6744	T10031	T10030	compound	BCA,method
R6745	T10032	T10033	punct	(,BioRad
R6746	T10033	T10030	parataxis	BioRad,method
R6747	T10034	T10033	punct	", ",BioRad
R6748	T10035	T10033	npadvmod	Hercules,BioRad
R6749	T10036	T10033	punct	", ",BioRad
R675	T1391	T1392	amod	internal,repeat
R6750	T10037	T10033	npadvmod	California,BioRad
R6751	T10038	T10033	punct	", ",BioRad
R6752	T10039	T10040	compound	United,States
R6753	T10040	T10033	npadvmod	States,BioRad
R6754	T10041	T10033	punct	),BioRad
R6755	T10042	T10026	advcl	using,measured
R6756	T10043	T10042	dobj	BSA,using
R6757	T10044	T10042	prep	as,using
R6758	T10045	T10046	det	a,standard
R6759	T10046	T10044	pobj	standard,as
R676	T1392	T1390	nmod	repeat,domains
R6760	T10047	T10026	punct	.,measured
R6761	T10131	T10132	amod	Metabolic,assays
R6762	T10133	T10132	punct	.,assays
R6763	T10135	T10136	nummod	Two,groups
R6764	T10136	T10137	nsubjpass	groups,kept
R6765	T10138	T10136	prep	of,groups
R6766	T10139	T10140	amod	male,mice
R6767	T10140	T10138	pobj	mice,of
R6768	T10141	T10137	auxpass	were,kept
R6769	T10142	T10137	prep	under,kept
R677	T1393	T1394	punct	(,IR
R6770	T10143	T10144	amod	separate,diet
R6771	T10144	T10142	pobj	diet,under
R6772	T10145	T10137	prep	since,kept
R6773	T10146	T10145	pobj	gestation,since
R6774	T10147	T10146	cc	or,gestation
R6775	T10148	T10146	conj	birth,gestation
R6776	T10149	T10137	punct	.,kept
R6777	T10151	T10152	nummod	One,group
R6778	T10152	T10153	nsubjpass	group,fed
R6779	T10154	T10153	auxpass	was,fed
R678	T1394	T1392	parataxis	IR,repeat
R6780	T10155	T10153	prep	with,fed
R6781	T10156	T10157	det	a,diet
R6782	T10157	T10155	pobj	diet,with
R6783	T10158	T10157	amod	normal,diet
R6784	T10159	T10157	compound	chow,diet
R6785	T10160	T10157	punct	(,diet
R6786	T10161	T10162	compound	PicoLab,Diet
R6787	T10162	T10157	appos	Diet,diet
R6788	T10163	T10162	compound	Rodent,Diet
R6789	T10164	T10162	nummod	20,Diet
R679	T1395	T1394	compound	W1W2,IR
R6790	T10165	T10162	punct	−,Diet
R6791	T10166	T10162	nummod	5053,Diet
R6792	T10167	T10168	punct	;,LabDiet
R6793	T10168	T10157	parataxis	LabDiet,diet
R6794	T10169	T10168	punct	", ",LabDiet
R6795	T10170	T10168	npadvmod	Richmond,LabDiet
R6796	T10171	T10168	punct	", ",LabDiet
R6797	T10172	T10168	npadvmod	Indiana,LabDiet
R6798	T10173	T10168	punct	", ",LabDiet
R6799	T10174	T10175	compound	United,States
R68	T303	T297	punct	),role
R680	T1396	T1394	punct	/,IR
R6800	T10175	T10168	npadvmod	States,LabDiet
R6801	T10176	T10168	punct	),LabDiet
R6802	T10177	T10153	punct	", ",fed
R6803	T10178	T10179	mark	while,placed
R6804	T10179	T10153	advcl	placed,fed
R6805	T10180	T10181	det	the,other
R6806	T10181	T10179	nsubjpass	other,placed
R6807	T10182	T10179	auxpass	was,placed
R6808	T10183	T10179	prep	on,placed
R6809	T10184	T10185	det	a,diet
R681	T1397	T1394	punct	),IR
R6810	T10185	T10183	pobj	diet,on
R6811	T10186	T10187	amod	higher,energy
R6812	T10187	T10185	nmod	energy,diet
R6813	T10188	T10187	cc	and,energy
R6814	T10189	T10187	conj	fat,energy
R6815	T10190	T10185	punct	(,diet
R6816	T10191	T10192	compound	PicoLab,Diet
R6817	T10192	T10185	appos	Diet,diet
R6818	T10193	T10192	compound	Mouse,Diet
R6819	T10194	T10192	nummod	20,Diet
R682	T1398	T1392	punct	", ",repeat
R6820	T10195	T10192	punct	−,Diet
R6821	T10196	T10192	nummod	5058,Diet
R6822	T10197	T10198	punct	;,LabDiet
R6823	T10198	T10185	parataxis	LabDiet,diet
R6824	T10199	T10198	punct	),LabDiet
R6825	T10200	T10153	punct	.,fed
R6826	T10202	T10203	compound	Glucose,tolerance
R6827	T10203	T10204	compound	tolerance,test
R6828	T10204	T10205	nsubjpass	test,performed
R6829	T10206	T10205	auxpass	was,performed
R683	T1399	T1392	cc	and,repeat
R6830	T10207	T10205	prep	on,performed
R6831	T10208	T10209	amod	overnight,fasted
R6832	T10209	T10211	amod	fasted,mice
R6833	T10210	T10209	punct	-,fasted
R6834	T10211	T10207	pobj	mice,on
R6835	T10212	T10211	acl	injected,mice
R6836	T10213	T10212	advmod	intraperitoneally,injected
R6837	T10214	T10212	prep	with,injected
R6838	T10215	T10216	nummod	2,g
R6839	T10216	T10214	pobj	g,with
R684	T1400	T1401	compound	zinc,finger
R6840	T10217	T10216	prep	of,g
R6841	T10218	T10217	pobj	glucose,of
R6842	T10219	T10216	prep	per,g
R6843	T10220	T10219	pobj	kg,per
R6844	T10221	T10220	prep	of,kg
R6845	T10222	T10223	compound	body,weight
R6846	T10223	T10221	pobj	weight,of
R6847	T10224	T10205	punct	.,performed
R6848	T10226	T10227	amod	Venous,values
R6849	T10227	T10230	nsubjpass	values,determined
R685	T1401	T1403	npadvmod	finger,rich
R6850	T10228	T10229	compound	blood,glucose
R6851	T10229	T10227	compound	glucose,values
R6852	T10231	T10230	auxpass	were,determined
R6853	T10232	T10230	prep	at,determined
R6854	T10233	T10234	advmod	immediately,before
R6855	T10234	T10232	prep	before,at
R6856	T10235	T10236	punct	(,min
R6857	T10236	T10234	parataxis	min,before
R6858	T10237	T10236	nummod	0,min
R6859	T10238	T10236	punct	),min
R686	T1402	T1401	punct	-,finger
R6860	T10239	T10234	punct	", ",before
R6861	T10240	T10234	cc	and,before
R6862	T10241	T10242	nummod	15,min
R6863	T10242	T10250	npadvmod	min,injection
R6864	T10243	T10241	punct	", ",15
R6865	T10244	T10241	conj	30,15
R6866	T10245	T10244	punct	", ",30
R6867	T10246	T10244	conj	60,30
R6868	T10247	T10246	punct	", ",60
R6869	T10248	T10246	cc	and,60
R687	T1403	T1392	conj	rich,repeat
R6870	T10249	T10246	conj	120,60
R6871	T10250	T10234	conj	injection,before
R6872	T10251	T10242	prep	after,min
R6873	T10252	T10250	det	the,injection
R6874	T10253	T10250	prep	with,injection
R6875	T10254	T10255	det	an,instrument
R6876	T10255	T10253	pobj	instrument,with
R6877	T10256	T10255	amod	automatic,instrument
R6878	T10257	T10255	compound	glucose,instrument
R6879	T10258	T10255	compound	monitor,instrument
R688	T1404	T1405	punct	(,ZnF
R6880	T10259	T10255	punct	(,instrument
R6881	T10260	T10261	nummod	One,Ultra
R6882	T10261	T10255	appos	Ultra,instrument
R6883	T10262	T10261	compound	Touch,Ultra
R6884	T10263	T10264	punct	", ",Lifescan
R6885	T10264	T10255	parataxis	Lifescan,instrument
R6886	T10265	T10264	punct	),Lifescan
R6887	T10266	T10230	punct	.,determined
R6888	T10268	T10269	compound	Insulin,tolerance
R6889	T10269	T10270	compound	tolerance,test
R689	T1405	T1403	parataxis	ZnF,rich
R6890	T10270	T10271	nsubjpass	test,performed
R6891	T10272	T10271	auxpass	was,performed
R6892	T10273	T10274	det	the,way
R6893	T10274	T10271	npadvmod	way,performed
R6894	T10275	T10274	amod	same,way
R6895	T10276	T10274	prep	as,way
R6896	T10277	T10276	pobj	that,as
R6897	T10278	T10277	acl	described,that
R6898	T10279	T10278	prep	for,described
R6899	T10280	T10281	det	the,test
R69	T304	T297	prep	of,role
R690	T1406	T1405	punct	),ZnF
R6900	T10281	T10279	pobj	test,for
R6901	T10282	T10283	compound	glucose,tolerance
R6902	T10283	T10281	compound	tolerance,test
R6903	T10284	T10274	prep	with,way
R6904	T10285	T10286	det	the,exception
R6905	T10286	T10284	pobj	exception,with
R6906	T10287	T10288	mark	that,injected
R6907	T10288	T10286	acl	injected,exception
R6908	T10289	T10290	amod	human,insulin
R6909	T10290	T10288	nsubjpass	insulin,injected
R691	T1407	T1381	prep	of,domain
R6910	T10291	T10292	punct	(,U
R6911	T10292	T10290	parataxis	U,insulin
R6912	T10293	T10292	nummod	0.75,U
R6913	T10294	T10295	punct	/,kg
R6914	T10295	T10292	prep	kg,U
R6915	T10296	T10295	prep	of,kg
R6916	T10297	T10298	compound	body,mass
R6917	T10298	T10296	pobj	mass,of
R6918	T10299	T10292	punct	),U
R6919	T10300	T10288	auxpass	was,injected
R692	T1408	T1407	pobj	RanBP2,of
R6920	T10301	T10288	advmod	intraperitoneally,injected
R6921	T10302	T10288	prep	in,injected
R6922	T10303	T10304	amod	non-fasted,mice
R6923	T10304	T10302	pobj	mice,in
R6924	T10305	T10271	punct	.,performed
R6925	T10307	T10308	compound	Pyruvate,challenge
R6926	T10308	T10309	compound	challenge,test
R6927	T10309	T10310	nsubjpass	test,performed
R6928	T10311	T10310	auxpass	was,performed
R6929	T10312	T10313	det	the,way
R693	T1409	T1377	advmod	specifically,associate
R6930	T10313	T10310	npadvmod	way,performed
R6931	T10314	T10313	amod	same,way
R6932	T10315	T10313	prep	as,way
R6933	T10316	T10315	pobj	that,as
R6934	T10317	T10316	acl	described,that
R6935	T10318	T10317	prep	for,described
R6936	T10319	T10320	det	the,test
R6937	T10320	T10318	pobj	test,for
R6938	T10321	T10322	compound	glucose,tolerance
R6939	T10322	T10320	compound	tolerance,test
R694	T1410	T1377	prep	with,associate
R6940	T10323	T10313	prep	with,way
R6941	T10324	T10325	det	the,exception
R6942	T10325	T10323	pobj	exception,with
R6943	T10326	T10327	mark	that,injected
R6944	T10327	T10325	acl	injected,exception
R6945	T10328	T10327	nsubjpass	pyruvate,injected
R6946	T10329	T10328	acl	dissolved,pyruvate
R6947	T10330	T10329	prep	in,dissolved
R6948	T10331	T10330	pobj	saline,in
R6949	T10332	T10333	punct	(,g
R695	T1411	T1410	pobj	components,with
R6950	T10333	T10331	parataxis	g,saline
R6951	T10334	T10333	nummod	2,g
R6952	T10335	T10336	punct	/,kg
R6953	T10336	T10333	prep	kg,g
R6954	T10337	T10336	prep	of,kg
R6955	T10338	T10339	compound	body,mass
R6956	T10339	T10337	pobj	mass,of
R6957	T10340	T10333	punct	),g
R6958	T10341	T10327	auxpass	was,injected
R6959	T10342	T10327	advmod	intraperitoneally,injected
R696	T1412	T1411	prep	of,components
R6960	T10343	T10327	prep	in,injected
R6961	T10344	T10345	amod	overnight,fasted
R6962	T10345	T10347	amod	fasted,mice
R6963	T10346	T10345	punct	-,fasted
R6964	T10347	T10343	pobj	mice,in
R6965	T10348	T10310	punct	.,performed
R6966	T10408	T10407	punct	.,Electroretinography
R6967	T10410	T10411	nummod	Five,mice
R6968	T10411	T10428	nsubjpass	mice,kept
R6969	T10412	T10413	punct	~,6
R697	T1413	T1414	det	the,cap
R6970	T10413	T10414	nummod	6,mo
R6971	T10414	T10416	npadvmod	mo,old
R6972	T10415	T10414	punct	-,mo
R6973	T10416	T10411	amod	old,mice
R6974	T10417	T10416	punct	-,old
R6975	T10418	T10411	amod	male,mice
R6976	T10419	T10411	nmod	RanBP2,mice
R6977	T10420	T10419	punct	+,RanBP2
R6978	T10421	T10419	punct	/,RanBP2
R6979	T10422	T10419	punct	+,RanBP2
R698	T1414	T1412	pobj	cap,of
R6980	T10423	T10419	cc	and,RanBP2
R6981	T10424	T10419	conj	RanBP2,RanBP2
R6982	T10425	T10424	punct	+,RanBP2
R6983	T10426	T10424	punct	/,RanBP2
R6984	T10427	T10424	punct	−,RanBP2
R6985	T10429	T10411	prep	in,mice
R6986	T10430	T10431	compound	129ola,background
R6987	T10431	T10429	pobj	background,in
R6988	T10432	T10428	auxpass	were,kept
R6989	T10433	T10428	prep	in,kept
R699	T1415	T1414	compound	19S,cap
R6990	T10434	T10435	nmod	dim,light
R6991	T10435	T10433	pobj	light,in
R6992	T10436	T10435	amod	cyclic,light
R6993	T10437	T10438	punct	(,lux
R6994	T10438	T10435	parataxis	lux,light
R6995	T10439	T10438	nummod	10,lux
R6996	T10440	T10438	punct	),lux
R6997	T10441	T10428	prep	for,kept
R6998	T10442	T10443	nummod	2,wk
R6999	T10443	T10441	pobj	wk,for
R7	T236	T234	cc	and,Cox11
R70	T307	T308	det	a,model
R700	T1416	T1414	prep	of,cap
R7000	T10444	T10445	mark	before,recorded
R7001	T10445	T10428	advcl	recorded,kept
R7002	T10446	T10447	amod	electroretinographic,responses
R7003	T10447	T10445	nsubjpass	responses,recorded
R7004	T10448	T10445	auxpass	were,recorded
R7005	T10449	T10428	punct	.,kept
R7006	T10451	T10452	det	The,mice
R7007	T10452	T10453	nsubjpass	mice,anesthetized
R7008	T10454	T10453	auxpass	were,anesthetized
R7009	T10455	T10453	advmod	intraperitoneally,anesthetized
R701	T1417	T1418	det	the,proteasome
R7010	T10456	T10453	prep	with,anesthetized
R7011	T10457	T10456	pobj	ketamine,with
R7012	T10458	T10459	punct	(,mg
R7013	T10459	T10457	parataxis	mg,ketamine
R7014	T10460	T10459	nummod	80,mg
R7015	T10461	T10462	punct	/,kg
R7016	T10462	T10459	prep	kg,mg
R7017	T10463	T10459	punct	),mg
R7018	T10464	T10457	cc	and,ketamine
R7019	T10465	T10457	conj	xylazine,ketamine
R702	T1418	T1416	pobj	proteasome,of
R7020	T10466	T10467	punct	(,mg
R7021	T10467	T10465	parataxis	mg,xylazine
R7022	T10468	T10467	nummod	4,mg
R7023	T10469	T10470	punct	/,kg
R7024	T10470	T10467	prep	kg,mg
R7025	T10471	T10467	punct	),mg
R7026	T10472	T10453	punct	.,anesthetized
R7027	T10474	T10475	compound	Body,temperature
R7028	T10475	T10476	nsubjpass	temperature,maintained
R7029	T10477	T10476	auxpass	was,maintained
R703	T1419	T1420	punct	[,14
R7030	T10478	T10476	prep	with,maintained
R7031	T10479	T10480	det	a,pad
R7032	T10480	T10478	pobj	pad,with
R7033	T10481	T10480	compound	heating,pad
R7034	T10482	T10476	prep	at,maintained
R7035	T10483	T10484	quantmod	37.5,1
R7036	T10484	T10486	nummod	1,°C
R7037	T10485	T10484	punct	±,1
R7038	T10486	T10482	pobj	°C,at
R7039	T10487	T10476	prep	throughout,maintained
R704	T1420	T1414	parataxis	14,cap
R7040	T10488	T10489	det	the,recording
R7041	T10489	T10487	pobj	recording,throughout
R7042	T10490	T10476	punct	.,maintained
R7043	T10492	T10493	det	The,pupils
R7044	T10493	T10494	nsubjpass	pupils,dilated
R7045	T10495	T10494	auxpass	were,dilated
R7046	T10496	T10494	advmod	fully,dilated
R7047	T10497	T10494	prep	with,dilated
R7048	T10498	T10499	nummod	0.5,%
R7049	T10499	T10500	compound	%,tropicamide
R705	T1421	T1420	punct	],14
R7050	T10500	T10497	pobj	tropicamide,with
R7051	T10501	T10500	cc	and,tropicamide
R7052	T10502	T10503	nummod	2.5,%
R7053	T10503	T10504	compound	%,HCl
R7054	T10504	T10500	conj	HCl,tropicamide
R7055	T10505	T10504	compound	phenylephrine,HCl
R7056	T10506	T10494	punct	", ",dilated
R7057	T10507	T10494	cc	and,dilated
R7058	T10508	T10509	det	both,eyes
R7059	T10509	T10510	nsubjpass	eyes,recorded
R706	T1422	T1414	punct	", ",cap
R7060	T10510	T10494	conj	recorded,dilated
R7061	T10511	T10510	auxpass	were,recorded
R7062	T10512	T10510	advmod	simultaneously,recorded
R7063	T10513	T10510	punct	.,recorded
R7064	T10515	T10516	amod	Corneal,loops
R7065	T10516	T10520	nsubjpass	loops,used
R7066	T10517	T10518	amod	gold,wire
R7067	T10518	T10516	compound	wire,loops
R7068	T10519	T10518	punct	-,wire
R7069	T10521	T10520	auxpass	were,used
R707	T1423	T1424	det	the,enzyme
R7070	T10522	T10520	prep	as,used
R7071	T10523	T10524	det	the,electrodes
R7072	T10524	T10522	pobj	electrodes,as
R7073	T10525	T10524	amod	active,electrodes
R7074	T10526	T10520	prep	under,used
R7075	T10527	T10528	nummod	0.5,%
R7076	T10528	T10529	nmod	%,hydrochloride
R7077	T10529	T10531	nmod	hydrochloride,anesthesia
R7078	T10530	T10529	nmod	proparacaine,hydrochloride
R7079	T10531	T10526	pobj	anesthesia,under
R708	T1424	T1414	conj	enzyme,cap
R7080	T10532	T10531	amod	topical,anesthesia
R7081	T10533	T10520	punct	.,used
R7082	T10535	T10536	amod	Gold,wire
R7083	T10536	T10538	compound	wire,electrodes
R7084	T10537	T10536	punct	-,wire
R7085	T10538	T10539	nsubj	electrodes,served
R7086	T10540	T10538	acl	placed,electrodes
R7087	T10541	T10540	prep	on,placed
R7088	T10542	T10543	det	the,sclera
R7089	T10543	T10541	pobj	sclera,on
R709	T1425	T1424	nmod	E2,enzyme
R7090	T10544	T10543	prep	near,sclera
R7091	T10545	T10546	det	the,limbus
R7092	T10546	T10544	pobj	limbus,near
R7093	T10547	T10539	prep	as,served
R7094	T10548	T10549	compound	reference,electrodes
R7095	T10549	T10547	pobj	electrodes,as
R7096	T10550	T10539	punct	.,served
R7097	T10552	T10553	det	A,wire
R7098	T10553	T10555	nsubjpass	wire,attached
R7099	T10554	T10553	compound	ground,wire
R71	T308	T306	pobj	model,in
R710	T1426	T1427	npadvmod	SUMO,conjugating
R7100	T10556	T10555	auxpass	was,attached
R7101	T10557	T10555	prep	to,attached
R7102	T10558	T10559	det	the,ear
R7103	T10559	T10557	pobj	ear,to
R7104	T10560	T10555	punct	.,attached
R7105	T10562	T10563	amod	Scotopic,responses
R7106	T10563	T10564	nsubjpass	responses,recorded
R7107	T10565	T10564	auxpass	were,recorded
R7108	T10566	T10564	prep	from,recorded
R7109	T10567	T10566	pobj	threshold,from
R711	T1427	T1424	amod	conjugating,enzyme
R7110	T10568	T10566	prep	to,from
R7111	T10569	T10570	nummod	9,units
R7112	T10570	T10568	pobj	units,to
R7113	T10571	T10570	compound	log,units
R7114	T10572	T10570	prep	of,units
R7115	T10573	T10572	pobj	intensity,of
R7116	T10574	T10570	prep	above,units
R7117	T10575	T10574	pobj	threshold,above
R7118	T10576	T10564	punct	", ",recorded
R7119	T10577	T10564	conj	amplified,recorded
R712	T1428	T1426	punct	-,SUMO
R7120	T10578	T10577	punct	", ",amplified
R7121	T10579	T10577	cc	and,amplified
R7122	T10580	T10577	conj	filtered,amplified
R7123	T10581	T10582	punct	(,Hz
R7124	T10582	T10580	parataxis	Hz,filtered
R7125	T10583	T10584	nummod	"5,000",gain
R7126	T10584	T10582	dep	gain,Hz
R7127	T10585	T10582	punct	", ",Hz
R7128	T10586	T10587	quantmod	0.1,"1,000"
R7129	T10587	T10582	nummod	"1,000",Hz
R713	T1429	T1426	nummod	1,SUMO
R7130	T10588	T10587	punct	–,"1,000"
R7131	T10589	T10582	punct	),Hz
R7132	T10590	T10564	punct	.,recorded
R7133	T10592	T10593	prep	For,used
R7134	T10594	T10595	amod	photopic,recordings
R7135	T10595	T10592	pobj	recordings,For
R7136	T10596	T10597	compound	background,light
R7137	T10597	T10593	nsubjpass	light,used
R7138	T10598	T10597	prep	at,light
R7139	T10599	T10600	nummod	34,cd
R714	T1430	T1426	punct	-,SUMO
R7140	T10600	T10598	pobj	cd,at
R7141	T10601	T10602	punct	/,m2
R7142	T10602	T10600	prep	m2,cd
R7143	T10603	T10593	auxpass	was,used
R7144	T10604	T10605	aux	to,suppress
R7145	T10605	T10593	advcl	suppress,used
R7146	T10606	T10607	compound	rod,function
R7147	T10607	T10605	dobj	function,suppress
R7148	T10608	T10593	punct	.,used
R715	T1431	T1424	punct	(,enzyme
R7150	T10852	T10853	det	The,LD
R7151	T10853	T10854	nsubj	LD,Interacts
R7152	T10855	T10853	prep	of,LD
R7153	T10856	T10855	pobj	RanBP2,of
R7154	T10857	T10854	prep	with,Interacts
R7155	T10858	T10857	pobj	Cox11,with
R7156	T10859	T10858	cc	and,Cox11
R7157	T10860	T10858	conj	HKI,Cox11
R7158	T10862	T10863	punct	(,A
R7159	T10863	T10864	meta	A,structure
R716	T1432	T1424	appos	Ubc9,enzyme
R7160	T10865	T10863	punct	),A
R7161	T10866	T10864	amod	Primary,structure
R7162	T10867	T10864	prep	of,structure
R7163	T10868	T10867	pobj	RanBP2,of
R7164	T10869	T10864	cc	and,structure
R7165	T10870	T10871	poss	its,domains
R7166	T10871	T10864	conj	domains,structure
R7167	T10872	T10873	amod	structural,functional
R7168	T10873	T10871	amod	functional,domains
R7169	T10874	T10873	punct	/,functional
R717	T1433	T1424	punct	),enzyme
R7170	T10875	T10871	punct	.,domains
R7171	T10877	T10878	det	The,LD
R7172	T10878	T10882	nsubjpass	LD,underlined
R7173	T10879	T10880	npadvmod	N,terminal
R7174	T10880	T10878	amod	terminal,LD
R7175	T10881	T10880	punct	-,terminal
R7176	T10883	T10878	prep	of,LD
R7177	T10884	T10883	pobj	RanBP2,of
R7178	T10885	T10882	auxpass	is,underlined
R7179	T10886	T10882	punct	.,underlined
R718	T1434	T1435	punct	[,15
R7180	T10888	T10889	punct	(,B
R7181	T10889	T10890	meta	B,alignment
R7182	T10891	T10889	punct	),B
R7183	T10892	T10890	compound	Sequence,alignment
R7184	T10893	T10890	prep	of,alignment
R7185	T10894	T10895	amod	murine,Cox11
R7186	T10895	T10893	pobj	Cox11,of
R7187	T10896	T10894	cc	and,murine
R7188	T10897	T10894	conj	yeast,murine
R7189	T10898	T10890	punct	.,alignment
R719	T1435	T1424	parataxis	15,enzyme
R7190	T10900	T10901	det	The,domains
R7191	T10901	T10910	nsubjpass	domains,conserved
R7192	T10902	T10901	nmod	yeast,domains
R7193	T10903	T10901	nmod	Cox11,domains
R7194	T10904	T10905	npadvmod	C,terminal
R7195	T10905	T10901	amod	terminal,domains
R7196	T10906	T10904	punct	-,C
R7197	T10907	T10904	cc	and,C
R7198	T10908	T10904	conj	N,C
R7199	T10909	T10905	punct	-,terminal
R72	T309	T310	amod	whole,animal
R720	T1436	T1435	punct	],15
R7200	T10911	T10910	auxpass	are,conserved
R7201	T10912	T10910	advmod	poorly,conserved
R7202	T10913	T10910	punct	.,conserved
R7203	T10915	T10916	nsubj	Arrow,denote
R7204	T10917	T10915	cc	and,Arrow
R7205	T10918	T10919	amod	solid,line
R7206	T10919	T10915	conj	line,Arrow
R7207	T10920	T10921	det	the,site
R7208	T10921	T10916	dobj	site,denote
R7209	T10922	T10921	amod	predicted,site
R721	T1437	T1424	punct	", ",enzyme
R7210	T10923	T10924	amod	mitochondrial,cleavage
R7211	T10924	T10921	compound	cleavage,site
R7212	T10925	T10921	cc	and,site
R7213	T10926	T10927	npadvmod	membrane,spanning
R7214	T10927	T10929	amod	spanning,domain
R7215	T10928	T10927	punct	-,spanning
R7216	T10929	T10921	conj	domain,site
R7217	T10930	T10916	punct	.,denote
R7218	T10932	T10933	det	The,lines
R7219	T10933	T10937	nsubj	lines,represent
R722	T1438	T1424	cc	and,enzyme
R7220	T10934	T10933	amod	dotted,lines
R7221	T10935	T10934	cc	and,dotted
R7222	T10936	T10934	conj	dashed,dotted
R7223	T10938	T10933	prep	above,lines
R7224	T10939	T10940	det	the,sequences
R7225	T10940	T10938	pobj	sequences,above
R7226	T10941	T10940	amod	aligned,sequences
R7227	T10942	T10937	punct	", ",represent
R7228	T10943	T10937	advmod	respectively,represent
R7229	T10944	T10937	punct	", ",represent
R723	T1439	T1440	det	the,receptor
R7230	T10945	T10946	nmod	Cox11,N
R7231	T10946	T10948	nmod	N,constructs
R7232	T10947	T10946	punct	-,N
R7233	T10948	T10937	dobj	constructs,represent
R7234	T10949	T10946	cc	and,N
R7235	T10950	T10951	compound	Cox11,C
R7236	T10951	T10946	conj	C,N
R7237	T10952	T10951	punct	-,C
R7238	T10953	T10948	acl	shown,constructs
R7239	T10954	T10953	prep	in,shown
R724	T1440	T1424	conj	receptor,enzyme
R7240	T10955	T10956	compound	Figure,1C
R7241	T10956	T10954	pobj	1C,in
R7242	T10957	T10937	punct	.,represent
R7243	T10959	T10960	punct	(,C
R7244	T10960	T10961	meta	C,analysis
R7245	T10962	T10960	punct	),C
R7246	T10963	T10964	compound	Structure,function
R7247	T10964	T10961	compound	function,analysis
R7248	T10965	T10964	punct	-,function
R7249	T10966	T10961	prep	of,analysis
R725	T1441	T1442	amod	nuclear,export
R7250	T10967	T10968	det	the,interaction
R7251	T10968	T10966	pobj	interaction,of
R7252	T10969	T10968	prep	between,interaction
R7253	T10970	T10971	det	the,LD
R7254	T10971	T10969	pobj	LD,between
R7255	T10972	T10971	prep	of,LD
R7256	T10973	T10972	pobj	RanBP2,of
R7257	T10974	T10971	cc	and,LD
R7258	T10975	T10971	conj	Cox11,LD
R7259	T10976	T10961	punct	.,analysis
R726	T1442	T1440	compound	export,receptor
R7260	T10978	T10979	amod	Optimal,interaction
R7261	T10979	T10980	nsubj	interaction,occurred
R7262	T10981	T10979	prep	between,interaction
R7263	T10982	T10983	det	the,LD
R7264	T10983	T10981	pobj	LD,between
R7265	T10984	T10983	cc	and,LD
R7266	T10985	T10983	conj	Cox11,LD
R7267	T10986	T10980	prep	in,occurred
R7268	T10987	T10988	det	the,presence
R7269	T10988	T10986	pobj	presence,in
R727	T1443	T1440	punct	", ",receptor
R7270	T10989	T10988	prep	of,presence
R7271	T10990	T10989	pobj	constructs,of
R7272	T10991	T10990	acl	comprising,constructs
R7273	T10992	T10993	preconj	both,LD
R7274	T10993	T10991	dobj	LD,comprising
R7275	T10994	T10993	det	the,LD
R7276	T10995	T10993	amod	complete,LD
R7277	T10996	T10993	cc	and,LD
R7278	T10997	T10993	conj	Cox11,LD
R7279	T10998	T10980	punct	.,occurred
R728	T1444	T1445	compound	CRM1,exportin
R7280	T11000	T11001	mark	Although,decreased
R7281	T11001	T11015	advcl	decreased,retained
R7282	T11002	T11001	nsubj	removal,decreased
R7283	T11003	T11002	prep	of,removal
R7284	T11004	T11005	det	the,N
R7285	T11005	T11003	pobj	N,of
R7286	T11006	T11005	amod	cytosolic,N
R7287	T11007	T11005	punct	-,N
R7288	T11008	T11005	amod	terminal,N
R7289	T11009	T11002	punct	(,removal
R729	T1445	T1440	appos	exportin,receptor
R7290	T11010	T11011	compound	Cox11,C
R7291	T11011	T11002	appos	C,removal
R7292	T11012	T11011	punct	-,C
R7293	T11013	T11001	punct	),decreased
R7294	T11014	T11001	advmod	significantly,decreased
R7295	T11016	T11017	det	the,interaction
R7296	T11017	T11001	dobj	interaction,decreased
R7297	T11018	T11017	prep	with,interaction
R7298	T11019	T11018	pobj	LD,with
R7299	T11020	T11015	punct	", ",retained
R73	T310	T308	compound	animal,model
R730	T1446	T1445	punct	/,exportin
R7300	T11021	T11022	det	the,domain
R7301	T11022	T11015	nsubj	domain,retained
R7302	T11023	T11022	amod	mitochondrial,domain
R7303	T11024	T11022	compound	intermembrane,domain
R7304	T11025	T11022	prep	of,domain
R7305	T11026	T11025	pobj	Cox11,of
R7306	T11027	T11022	punct	(,domain
R7307	T11028	T11029	compound	Cox11,C
R7308	T11029	T11022	appos	C,domain
R7309	T11030	T11029	punct	-,C
R731	T1447	T1445	punct	-,exportin
R7310	T11031	T11022	punct	),domain
R7311	T11032	T11022	advmod	together,domain
R7312	T11033	T11032	prep	with,together
R7313	T11034	T11035	det	the,half
R7314	T11035	T11033	pobj	half,with
R7315	T11036	T11037	npadvmod	C,terminal
R7316	T11037	T11035	amod	terminal,half
R7317	T11038	T11037	punct	-,terminal
R7318	T11039	T11035	prep	of,half
R7319	T11040	T11039	pobj	LD,of
R732	T1448	T1445	nummod	1,exportin
R7320	T11041	T11035	punct	(,half
R7321	T11042	T11043	compound	LD,C
R7322	T11043	T11035	appos	C,half
R7323	T11044	T11043	punct	-,C
R7324	T11045	T11015	punct	),retained
R7325	T11046	T11015	dobj	most,retained
R7326	T11047	T11046	prep	of,most
R7327	T11048	T11049	det	the,activity
R7328	T11049	T11047	pobj	activity,of
R7329	T11050	T11049	compound	interaction,activity
R733	T1449	T1450	punct	[,16
R7330	T11051	T11015	punct	.,retained
R7331	T11053	T11054	compound	LD,N
R7332	T11054	T11056	nsubj	N,ended
R7333	T11055	T11054	punct	-,N
R7334	T11057	T11054	cc	and,N
R7335	T11058	T11059	compound	LD,C
R7336	T11059	T11054	conj	C,N
R7337	T11060	T11059	punct	-,C
R7338	T11061	T11056	cc	and,ended
R7339	T11062	T11056	conj	began,ended
R734	T1450	T1440	parataxis	16,receptor
R7340	T11063	T11062	prep	with,began
R7341	T11064	T11065	det	the,domain
R7342	T11065	T11063	pobj	domain,with
R7343	T11066	T11067	compound	leucine,zipper
R7344	T11067	T11065	compound	zipper,domain
R7345	T11068	T11065	prep	of,domain
R7346	T11069	T11068	pobj	RanBP2,of
R7347	T11070	T11056	punct	.,ended
R7348	T11072	T11073	amod	White,bars
R7349	T11073	T11076	nsubj	bars,denote
R735	T1451	T1450	punct	],16
R7350	T11074	T11072	cc	and,White
R7351	T11075	T11072	conj	black,White
R7352	T11077	T11078	compound	β,galactosidase
R7353	T11078	T11080	compound	galactosidase,activity
R7354	T11079	T11078	punct	-,galactosidase
R7355	T11080	T11076	dobj	activity,denote
R7356	T11081	T11080	cc	and,activity
R7357	T11082	T11083	compound	growth,rates
R7358	T11083	T11080	conj	rates,activity
R7359	T11084	T11083	prep	in,rates
R736	T1452	T1377	punct	", ",associate
R7360	T11085	T11086	amod	selective,medium
R7361	T11086	T11084	pobj	medium,in
R7362	T11087	T11086	compound	growth,medium
R7363	T11088	T11076	punct	", ",denote
R7364	T11089	T11076	advmod	respectively,denote
R7365	T11090	T11076	punct	.,denote
R7366	T11092	T11093	nsubj	Results,represent
R7367	T11093	T11095	ccomp	represent,3
R7368	T11094	T11092	acl	shown,Results
R7369	T11096	T11097	det	the,SD
R737	T1453	T1377	advmod	respectively,associate
R7370	T11097	T11093	dobj	SD,represent
R7371	T11098	T11097	nmod	mean,SD
R7372	T11099	T11097	punct	±,SD
R7373	T11100	T11095	punct	", ",3
R7374	T11101	T11095	nsubj	n,3
R7375	T11102	T11095	punct	=,3
R7376	T11103	T11095	punct	.,3
R7377	T11105	T11106	punct	(,D
R7378	T11106	T11107	meta	D,assays
R7379	T11108	T11106	punct	),D
R738	T1454	T1377	punct	.,associate
R7380	T11109	T11107	nmod	GST,assays
R7381	T11110	T11107	amod	pull,assays
R7382	T11111	T11110	punct	-,pull
R7383	T11112	T11110	prt	down,pull
R7384	T11113	T11107	prep	with,assays
R7385	T11114	T11115	det	the,LD
R7386	T11115	T11113	pobj	LD,with
R7387	T11116	T11115	prep	of,LD
R7388	T11117	T11116	pobj	RanBP2,of
R7389	T11118	T11115	cc	and,LD
R739	T1456	T1457	nsubjpass	RanBP2,found
R7390	T11119	T11120	poss	its,domain
R7391	T11120	T11115	conj	domain,LD
R7392	T11121	T11122	compound	leucine,zipper
R7393	T11122	T11120	compound	zipper,domain
R7394	T11123	T11120	cc	and,domain
R7395	T11124	T11125	amod	retinal,extracts
R7396	T11125	T11120	conj	extracts,domain
R7397	T11126	T11107	punct	.,assays
R7398	T11128	T11129	det	The,LD
R7399	T11129	T11130	nsubj	LD,associate
R74	T311	T310	punct	-,animal
R740	T1458	T1457	npadvmod	itself,found
R7400	T11131	T11129	punct	", ",LD
R7401	T11132	T11129	cc	but,LD
R7402	T11133	T11132	neg	not,but
R7403	T11134	T11135	det	the,domain
R7404	T11135	T11129	conj	domain,LD
R7405	T11136	T11137	compound	leucine,zipper
R7406	T11137	T11135	compound	zipper,domain
R7407	T11138	T11129	prep	of,LD
R7408	T11139	T11138	pobj	RanBP2,of
R7409	T11140	T11130	punct	", ",associate
R741	T1459	T1457	auxpass	was,found
R7410	T11141	T11130	prep	with,associate
R7411	T11142	T11141	pobj	Cox11,with
R7412	T11143	T11144	punct	(,lane
R7413	T11144	T11142	parataxis	lane,Cox11
R7414	T11145	T11146	amod	top,panel
R7415	T11146	T11144	dep	panel,lane
R7416	T11147	T11144	punct	", ",lane
R7417	T11148	T11144	nummod	1,lane
R7418	T11149	T11144	punct	),lane
R7419	T11150	T11142	cc	and,Cox11
R742	T1460	T1457	advmod	also,found
R7420	T11151	T11142	conj	HKI,Cox11
R7421	T11152	T11153	punct	(,lane
R7422	T11153	T11151	parataxis	lane,HKI
R7423	T11154	T11155	amod	bottom,panel
R7424	T11155	T11153	dep	panel,lane
R7425	T11156	T11153	punct	", ",lane
R7426	T11157	T11153	nummod	1,lane
R7427	T11158	T11153	punct	),lane
R7428	T11159	T11130	punct	.,associate
R7429	T11161	T11162	punct	(,E
R743	T1461	T1462	aux	to,exhibit
R7430	T11162	T11163	meta	E,shows
R7431	T11164	T11162	punct	),E
R7432	T11165	T11163	nsubj	Coimmunoprecipitation,shows
R7433	T11166	T11165	prep	of,Coimmunoprecipitation
R7434	T11167	T11166	pobj	RanBP2,of
R7435	T11168	T11165	prep	with,Coimmunoprecipitation
R7436	T11169	T11168	pobj	antibodies,with
R7437	T11170	T11169	prep	against,antibodies
R7438	T11171	T11172	poss	its,partners
R7439	T11172	T11170	pobj	partners,against
R744	T1462	T1457	xcomp	exhibit,found
R7440	T11173	T11172	amod	molecular,partners
R7441	T11174	T11175	mark	that,forms
R7442	T11175	T11163	ccomp	forms,shows
R7443	T11176	T11175	nsubj	RanBP2,forms
R7444	T11177	T11178	det	a,complex
R7445	T11178	T11175	dobj	complex,forms
R7446	T11179	T11180	advmod	in,vivo
R7447	T11180	T11175	advmod	vivo,forms
R7448	T11181	T11175	prep	with,forms
R7449	T11182	T11181	pobj	HKI,with
R745	T1463	T1464	compound	SUMO1,ligase
R7450	T11183	T11184	punct	(,1
R7451	T11184	T11182	parataxis	1,HKI
R7452	T11185	T11184	nmod	lanes,1
R7453	T11186	T11184	cc	and,1
R7454	T11187	T11184	conj	2,1
R7455	T11188	T11184	punct	),1
R7456	T11189	T11182	punct	", ",HKI
R7457	T11190	T11182	conj	mHsp70,HKI
R7458	T11191	T11192	punct	(,lane
R7459	T11192	T11190	parataxis	lane,mHsp70
R746	T1464	T1466	compound	ligase,activity
R7460	T11193	T11192	nummod	3,lane
R7461	T11194	T11192	punct	),lane
R7462	T11195	T11190	punct	", ",mHsp70
R7463	T11196	T11190	cc	and,mHsp70
R7464	T11197	T11190	conj	Cox11,mHsp70
R7465	T11198	T11199	punct	(,lane
R7466	T11199	T11197	parataxis	lane,Cox11
R7467	T11200	T11199	nummod	4,lane
R7468	T11201	T11199	punct	),lane
R7469	T11202	T11163	punct	.,shows
R747	T1465	T1464	compound	E3,ligase
R7470	T11204	T11205	nmod	Lanes,5
R7471	T11205	T11206	nsubj	5,are
R7472	T11207	T11205	punct	", ",5
R7473	T11208	T11205	conj	6,5
R7474	T11209	T11208	punct	", ",6
R7475	T11210	T11208	cc	and,6
R7476	T11211	T11208	conj	7,6
R7477	T11212	T11213	compound	control,reactions
R7478	T11213	T11206	attr	reactions,are
R7479	T11214	T11213	compound	immunoprecipitation,reactions
R748	T1466	T1462	dobj	activity,exhibit
R7480	T11215	T11213	prep	with,reactions
R7481	T11216	T11217	amod	different,antibodies
R7482	T11217	T11215	pobj	antibodies,with
R7483	T11218	T11217	prep	against,antibodies
R7484	T11219	T11220	det	the,domains
R7485	T11220	T11218	pobj	domains,against
R7486	T11221	T11220	compound	RanBP2,domains
R7487	T11222	T11220	punct	", ",domains
R7488	T11223	T11220	appos	KBD,domains
R7489	T11224	T11223	punct	", ",KBD
R749	T1467	T1468	punct	[,17
R7490	T11225	T11223	conj	ZnF,KBD
R7491	T11226	T11225	punct	", ",ZnF
R7492	T11227	T11225	cc	and,ZnF
R7493	T11228	T11225	conj	XAFXFG,ZnF
R7494	T11229	T11220	prep	of,domains
R7495	T11230	T11229	pobj	nucleoporins,of
R7496	T11231	T11206	punct	.,are
R7497	T11233	T11234	punct	(,F
R7498	T11234	T11235	meta	F,coimmunoprecipitation
R7499	T11236	T11234	punct	),F
R75	T312	T286	acomp	elusive,remain
R750	T1468	T1462	parataxis	17,exhibit
R7500	T11237	T11235	amod	Reciprocal,coimmunoprecipitation
R7501	T11238	T11235	prep	of,coimmunoprecipitation
R7502	T11239	T11238	pobj	HKI,of
R7503	T11240	T11235	prep	with,coimmunoprecipitation
R7504	T11241	T11240	pobj	antibodies,with
R7505	T11242	T11241	prep	against,antibodies
R7506	T11243	T11242	pobj	RanBP2,against
R7507	T11244	T11241	punct	(,antibodies
R7508	T11245	T11241	acl	used,antibodies
R7509	T11246	T11245	cc	and,used
R751	T1469	T1468	punct	],17
R7510	T11247	T11245	conj	shown,used
R7511	T11248	T11247	prep	in,shown
R7512	T11249	T11248	punct	(,in
R7513	T11250	T11248	pobj	E,in
R7514	T11251	T11235	punct	),coimmunoprecipitation
R7515	T11252	T11235	punct	),coimmunoprecipitation
R7516	T11253	T11235	punct	.,coimmunoprecipitation
R7517	T11255	T11256	punct	(,G
R7518	T11256	T11257	meta	G,assays
R7519	T11258	T11256	punct	),G
R752	T1470	T1462	punct	", ",exhibit
R7520	T11259	T11257	nmod	Reconstitution,assays
R7521	T11260	T11257	amod	pull,assays
R7522	T11261	T11260	punct	-,pull
R7523	T11262	T11260	prt	down,pull
R7524	T11263	T11257	prep	with,assays
R7525	T11264	T11265	amod	purified,LD
R7526	T11265	T11263	pobj	LD,with
R7527	T11266	T11265	cc	and,LD
R7528	T11267	T11268	amod	increasing,concentrations
R7529	T11268	T11265	conj	concentrations,LD
R753	T1471	T1462	advcl	supporting,exhibit
R7530	T11269	T11268	prep	of,concentrations
R7531	T11270	T11271	amod	native,Cox11
R7532	T11271	T11269	pobj	Cox11,of
R7533	T11272	T11273	punct	(,panel
R7534	T11273	T11270	parataxis	panel,native
R7535	T11274	T11273	amod	top,panel
R7536	T11275	T11273	punct	),panel
R7537	T11276	T11270	punct	", ",native
R7538	T11277	T11270	conj	denatured,native
R7539	T11278	T11279	punct	(,panel
R754	T1472	T1473	det	a,link
R7540	T11279	T11277	parataxis	panel,denatured
R7541	T11280	T11279	amod	middle,panel
R7542	T11281	T11279	punct	),panel
R7543	T11282	T11277	punct	", ",denatured
R7544	T11283	T11277	cc	and,denatured
R7545	T11284	T11285	advmod	partially,denatured
R7546	T11285	T11277	conj	denatured,denatured
R7547	T11286	T11287	punct	(,panel
R7548	T11287	T11285	parataxis	panel,denatured
R7549	T11288	T11287	amod	bottom,panel
R755	T1473	T1471	dobj	link,supporting
R7550	T11289	T11287	punct	),panel
R7551	T11290	T11268	punct	", ",concentrations
R7552	T11291	T11268	advmod	respectively,concentrations
R7553	T11292	T11257	punct	", ",assays
R7554	T11293	T11257	prep	in,assays
R7555	T11294	T11295	det	the,absence
R7556	T11295	T11293	pobj	absence,in
R7557	T11296	T11295	cc	and,absence
R7558	T11297	T11295	conj	presence,absence
R7559	T11298	T11295	prep	of,absence
R756	T1474	T1473	amod	direct,link
R7560	T11299	T11300	amod	denaturating,agent
R7561	T11300	T11298	pobj	agent,of
R7562	T11301	T11300	punct	", ",agent
R7563	T11302	T11300	appos	GnHCl,agent
R7564	T11303	T11300	cc	and,agent
R7565	T11304	T11305	amod	chaotropic,agent
R7566	T11305	T11300	conj	agent,agent
R7567	T11306	T11305	punct	", ",agent
R7568	T11307	T11305	appos	urea,agent
R7569	T11308	T11257	punct	.,assays
R757	T1475	T1473	prep	between,link
R7570	T11310	T11311	compound	Folding,intermediates
R7571	T11311	T11312	nsubj	intermediates,exhibit
R7572	T11313	T11314	punct	(,panel
R7573	T11314	T11311	parataxis	panel,intermediates
R7574	T11315	T11314	amod	lower,panel
R7575	T11316	T11314	punct	),panel
R7576	T11317	T11311	prep	of,intermediates
R7577	T11318	T11317	pobj	Cox11,of
R7578	T11319	T11320	det	the,activity
R7579	T11320	T11312	dobj	activity,exhibit
R758	T1476	T1477	npadvmod	RanBP2,mediated
R7580	T11321	T11320	amod	highest,activity
R7581	T11322	T11320	compound	binding,activity
R7582	T11323	T11320	prep	toward,activity
R7583	T11324	T11325	det	the,LD
R7584	T11325	T11323	pobj	LD,toward
R7585	T11326	T11325	prep	of,LD
R7586	T11327	T11326	pobj	RanBP2,of
R7587	T11328	T11312	punct	.,exhibit
R7588	T11330	T11331	punct	(,H
R7589	T11331	T11332	meta	H,experiments
R759	T1477	T1479	amod	mediated,modification
R7590	T11333	T11331	punct	),H
R7591	T11334	T11332	amod	Similar,experiments
R7592	T11335	T11332	prep	as,experiments
R7593	T11336	T11335	prep	in,as
R7594	T11337	T11336	punct	(,in
R7595	T11338	T11336	pobj	G,in
R7596	T11339	T11332	punct	),experiments
R7597	T11340	T11332	prep	but,experiments
R7598	T11341	T11340	prep	in,but
R7599	T11342	T11343	det	the,presence
R76	T313	T286	punct	.,remain
R760	T1478	T1477	punct	-,mediated
R7600	T11343	T11341	pobj	presence,in
R7601	T11344	T11343	prep	of,presence
R7602	T11345	T11346	amod	native,Cox11
R7603	T11346	T11344	pobj	Cox11,of
R7604	T11347	T11346	acl	expressed,Cox11
R7605	T11348	T11347	prep	in,expressed
R7606	T11349	T11350	det	the,absence
R7607	T11350	T11348	pobj	absence,in
R7608	T11351	T11352	punct	(,panel
R7609	T11352	T11350	parataxis	panel,absence
R761	T1479	T1475	pobj	modification,between
R7610	T11353	T11352	amod	top,panel
R7611	T11354	T11352	punct	),panel
R7612	T11355	T11350	cc	or,absence
R7613	T11356	T11350	conj	presence,absence
R7614	T11357	T11358	punct	(,panel
R7615	T11358	T11356	parataxis	panel,presence
R7616	T11359	T11358	amod	bottom,panel
R7617	T11360	T11358	punct	),panel
R7618	T11361	T11350	prep	of,absence
R7619	T11362	T11361	pobj	CuSO4,of
R762	T1480	T1481	nmod	SUMO,substrate
R7620	T11363	T11332	punct	.,experiments
R7621	T11365	T11366	det	The,isoform
R7622	T11366	T11368	nsubj	isoform,has
R7623	T11367	T11366	amod	mature,isoform
R7624	T11369	T11366	prep	of,isoform
R7625	T11370	T11371	det	the,metallochaperone
R7626	T11371	T11369	pobj	metallochaperone,of
R7627	T11372	T11373	det	an,affinity
R7628	T11373	T11368	dobj	affinity,has
R7629	T11374	T11373	amod	increased,affinity
R763	T1481	T1479	compound	substrate,modification
R7630	T11375	T11373	prep	toward,affinity
R7631	T11376	T11377	det	the,LD
R7632	T11377	T11375	pobj	LD,toward
R7633	T11378	T11377	prep	of,LD
R7634	T11379	T11378	pobj	RanBP2,of
R7635	T11380	T11368	punct	.,has
R7636	T11383	T11382	punct	", ",LD
R7637	T11384	T11385	npadvmod	leucine,rich
R7638	T11385	T11387	amod	rich,domain
R7639	T11386	T11385	punct	-,rich
R764	T1482	T1480	punct	-,SUMO
R7640	T11387	T11382	appos	domain,LD
R7641	T11388	T11382	punct	;,LD
R7642	T11389	T11382	appos	LZ,LD
R7643	T11390	T11389	punct	", ",LZ
R7644	T11391	T11392	compound	leucine,zipper
R7645	T11392	T11393	compound	zipper,domain
R7646	T11393	T11389	appos	domain,LZ
R7647	T11394	T11382	punct	;,LD
R7648	T11395	T11382	appos	RBD1,LD
R7649	T11396	T11397	punct	–,4
R765	T1483	T1480	nummod	1,SUMO
R7650	T11397	T11395	prep	4,RBD1
R7651	T11398	T11395	punct	", ",RBD1
R7652	T11399	T11400	compound	Ran,binding
R7653	T11400	T11402	compound	binding,domains
R7654	T11401	T11400	punct	-,binding
R7655	T11402	T11395	appos	domains,RBD1
R7656	T11403	T11404	quantmod	1,4
R7657	T11404	T11402	npadvmod	4,domains
R7658	T11405	T11404	punct	–,4
R7659	T11406	T11382	punct	;,LD
R766	T1484	T1479	cc	and,modification
R7660	T11407	T11382	appos	ZnF,LD
R7661	T11408	T11407	punct	", ",ZnF
R7662	T11409	T11410	compound	zinc,finger
R7663	T11410	T11411	compound	finger,domain
R7664	T11411	T11407	appos	domain,ZnF
R7665	T11412	T11411	compound	cluster,domain
R7666	T11413	T11382	punct	;,LD
R7667	T11414	T11382	appos	KBD,LD
R7668	T11415	T11414	punct	", ",KBD
R7669	T11416	T11417	nmod	kinesin,binding
R767	T1485	T1479	conj	relocation,modification
R7670	T11417	T11424	compound	binding,domain
R7671	T11418	T11416	punct	(,kinesin
R7672	T11419	T11420	compound	KIF5B,KIF5C
R7673	T11420	T11416	appos	KIF5C,kinesin
R7674	T11421	T11420	punct	/,KIF5C
R7675	T11422	T11417	punct	),binding
R7676	T11423	T11417	punct	-,binding
R7677	T11424	T11414	appos	domain,KBD
R7678	T11425	T11382	punct	;,LD
R7679	T11426	T11382	appos	CLD,LD
R768	T1486	T1485	prep	of,relocation
R7680	T11427	T11426	punct	", ",CLD
R7681	T11428	T11429	npadvmod	cyclophilin,like
R7682	T11429	T11431	amod	like,domain
R7683	T11430	T11429	punct	-,like
R7684	T11431	T11426	appos	domain,CLD
R7685	T11432	T11382	punct	;,LD
R7686	T11433	T11382	appos	IR,LD
R7687	T11434	T11433	punct	", ",IR
R7688	T11435	T11436	amod	internal,repeat
R7689	T11436	T11437	compound	repeat,domain
R769	T1487	T1488	npadvmod	SUMO,modified
R7690	T11437	T11433	appos	domain,IR
R7691	T11438	T11382	punct	;,LD
R7692	T11439	T11382	appos	CY,LD
R7693	T11440	T11439	punct	", ",CY
R7694	T11441	T11442	compound	cyclophilin,domain
R7695	T11442	T11439	appos	domain,CY
R7696	T11443	T11382	punct	.,LD
R7697	T11521	T11520	prep	of,Effect
R7698	T11522	T11521	pobj	Cox11,of
R7699	T11523	T11522	cc	and,Cox11
R77	T315	T316	advmod	Here,report
R770	T1488	T1491	amod	modified,substrates
R7700	T11524	T11522	conj	RanBP2,Cox11
R7701	T11525	T11520	prep	on,Effect
R7702	T11526	T11527	compound	HKI,Activity
R7703	T11527	T11525	pobj	Activity,on
R7704	T11529	T11530	punct	(,A
R7705	T11530	T11531	meta	A,kinetics
R7706	T11532	T11530	punct	),A
R7707	T11533	T11531	compound	Saturation,kinetics
R7708	T11534	T11531	punct	", ",kinetics
R7709	T11535	T11531	npadvmod	rate,kinetics
R771	T1489	T1487	punct	-,SUMO
R7710	T11536	T11535	cc	versus,rate
R7711	T11537	T11535	conj	glucose,rate
R7712	T11538	T11531	prep	of,kinetics
R7713	T11539	T11538	pobj	HKI,of
R7714	T11540	T11541	punct	(,μg
R7715	T11541	T11539	parataxis	μg,HKI
R7716	T11542	T11541	nummod	0.24,μg
R7717	T11543	T11541	punct	),μg
R7718	T11544	T11531	prep	in,kinetics
R7719	T11545	T11546	det	the,absence
R772	T1490	T1487	nummod	1,SUMO
R7720	T11546	T11544	pobj	absence,in
R7721	T11547	T11548	punct	(,circles
R7722	T11548	T11546	parataxis	circles,absence
R7723	T11549	T11548	amod	solid,circles
R7724	T11550	T11548	punct	),circles
R7725	T11551	T11546	cc	and,absence
R7726	T11552	T11546	conj	presence,absence
R7727	T11553	T11546	prep	of,absence
R7728	T11554	T11553	pobj	Cox11,of
R7729	T11555	T11556	punct	(,nM
R773	T1491	T1486	pobj	substrates,of
R7730	T11556	T11531	parataxis	nM,kinetics
R7731	T11557	T11558	amod	open,circles
R7732	T11558	T11559	dep	circles,nM
R7733	T11559	T11556	dep	nM,nM
R7734	T11560	T11559	punct	", ",nM
R7735	T11561	T11559	nummod	0.25,nM
R7736	T11562	T11556	punct	;,nM
R7737	T11563	T11564	amod	solid,triangles
R7738	T11564	T11556	dep	triangles,nM
R7739	T11565	T11556	punct	", ",nM
R774	T1492	T1491	amod	cytosolic,substrates
R7740	T11566	T11556	nummod	7.5,nM
R7741	T11567	T11556	punct	),nM
R7742	T11568	T11531	punct	.,kinetics
R7743	T11570	T11571	det	The,activity
R7744	T11571	T11572	nsubj	activity,decreases
R7745	T11573	T11571	prep	of,activity
R7746	T11574	T11573	pobj	HKI,of
R7747	T11575	T11572	prep	with,decreases
R7748	T11576	T11577	amod	increasing,concentrations
R7749	T11577	T11575	pobj	concentrations,with
R775	T1493	T1491	punct	(,substrates
R7750	T11578	T11577	prep	of,concentrations
R7751	T11579	T11578	pobj	Cox11,of
R7752	T11580	T11572	punct	.,decreases
R7753	T11582	T11583	det	No,activity
R7754	T11583	T11586	nsubjpass	activity,recorded
R7755	T11584	T11583	amod	measurable,activity
R7756	T11585	T11583	compound	HKI,activity
R7757	T11587	T11586	auxpass	was,recorded
R7758	T11588	T11586	prep	in,recorded
R7759	T11589	T11590	det	the,presence
R776	T1494	T1495	advmod	e.g.,RanGAP
R7760	T11590	T11588	pobj	presence,in
R7761	T11591	T11590	prep	of,presence
R7762	T11592	T11593	nummod	15,nM
R7763	T11593	T11591	pobj	nM,of
R7764	T11594	T11593	prep	of,nM
R7765	T11595	T11594	pobj	Cox11,of
R7766	T11596	T11597	punct	(,data
R7767	T11597	T11586	meta	data,recorded
R7768	T11598	T11597	amod	unpublished,data
R7769	T11599	T11597	punct	),data
R777	T1495	T1491	appos	RanGAP,substrates
R7770	T11600	T11586	punct	.,recorded
R7771	T11602	T11603	punct	(,B
R7772	T11603	T11604	meta	B,plot
R7773	T11605	T11603	punct	),B
R7774	T11606	T11607	compound	Hanes,Wolf
R7775	T11607	T11604	compound	Wolf,plot
R7776	T11608	T11607	punct	-,Wolf
R7777	T11609	T11604	prep	of,plot
R7778	T11610	T11611	punct	(,A
R7779	T11611	T11609	parataxis	A,of
R778	T1496	T1495	punct	", ",RanGAP
R7780	T11612	T11611	punct	),A
R7781	T11613	T11609	punct	(,of
R7782	T11614	T11609	pobj	1,of
R7783	T11615	T11616	punct	/,rate
R7784	T11616	T11614	prep	rate,1
R7785	T11617	T11614	cc	versus,1
R7786	T11618	T11614	conj	glucose,1
R7787	T11619	T11604	punct	),plot
R7788	T11620	T11604	prep	in,plot
R7789	T11621	T11622	det	the,absence
R779	T1497	T1485	punct	),relocation
R7790	T11622	T11620	pobj	absence,in
R7791	T11623	T11622	cc	and,absence
R7792	T11624	T11622	conj	presence,absence
R7793	T11625	T11622	prep	of,absence
R7794	T11626	T11627	amod	fixed,concentrations
R7795	T11627	T11625	pobj	concentrations,of
R7796	T11628	T11627	prep	of,concentrations
R7797	T11629	T11628	pobj	Cox11,of
R7798	T11630	T11604	punct	.,plot
R7799	T11632	T11633	nsubj	Linearity,supported
R78	T317	T316	punct	", ",report
R780	T1498	T1485	prep	to,relocation
R7800	T11634	T11632	prep	of,Linearity
R7801	T11635	T11636	amod	reciprocal,plots
R7802	T11636	T11634	pobj	plots,of
R7803	T11637	T11633	advmod	also,supported
R7804	T11638	T11639	det	the,behavior
R7805	T11639	T11633	dobj	behavior,supported
R7806	T11640	T11639	amod	hyperbolic,behavior
R7807	T11641	T11639	prep	of,behavior
R7808	T11642	T11643	det	the,reactions
R7809	T11643	T11641	pobj	reactions,of
R781	T1499	T1500	det	the,face
R7810	T11644	T11645	punct	(,data
R7811	T11645	T11633	meta	data,supported
R7812	T11646	T11645	amod	unpublished,data
R7813	T11647	T11645	punct	),data
R7814	T11648	T11633	punct	.,supported
R7815	T11650	T11651	nsubj	Cox11,behaves
R7816	T11652	T11651	prep	as,behaves
R7817	T11653	T11654	det	a,inhibitor
R7818	T11654	T11652	pobj	inhibitor,as
R7819	T11655	T11654	amod	noncompetitive,inhibitor
R782	T1500	T1498	pobj	face,to
R7820	T11656	T11654	prep	of,inhibitor
R7821	T11657	T11656	pobj	HKI,of
R7822	T11658	T11651	prep	by,behaves
R7823	T11659	T11658	pcomp	reducing,by
R7824	T11660	T11661	det	the,V max
R7825	T11661	T11659	dobj	V max,reducing
R7826	T11662	T11661	prep	of,V max
R7827	T11663	T11662	pobj	HKI,of
R7828	T11664	T11661	cc	but,V max
R7829	T11665	T11664	neg	not,but
R783	T1501	T1500	amod	cytosolic,face
R7830	T11666	T11667	poss	its,K m
R7831	T11667	T11661	conj	K m,V max
R7832	T11668	T11667	prep	toward,K m
R7833	T11669	T11668	pobj	glucose,toward
R7834	T11670	T11651	punct	.,behaves
R7835	T11672	T11673	punct	(,C
R7836	T11673	T11674	meta	C,plotted
R7837	T11675	T11673	punct	),C
R7838	T11676	T11677	compound	HKI,rate
R7839	T11677	T11674	nsubjpass	rate,plotted
R784	T1502	T1500	prep	of,face
R7840	T11678	T11674	auxpass	is,plotted
R7841	T11679	T11674	prep	as,plotted
R7842	T11680	T11681	det	a,function
R7843	T11681	T11679	pobj	function,as
R7844	T11682	T11681	prep	of,function
R7845	T11683	T11684	compound	LD,concentration
R7846	T11684	T11682	pobj	concentration,of
R7847	T11685	T11674	prep	at,plotted
R7848	T11686	T11687	amod	saturating,glucose
R7849	T11687	T11688	nmod	glucose,concentrations
R785	T1503	T1504	det	the,complex
R7850	T11688	T11685	pobj	concentrations,at
R7851	T11689	T11687	cc	and,glucose
R7852	T11690	T11691	amod	fixed,Cox11
R7853	T11691	T11687	conj	Cox11,glucose
R7854	T11692	T11693	punct	(,nM
R7855	T11693	T11691	parataxis	nM,Cox11
R7856	T11694	T11693	nummod	7.5,nM
R7857	T11695	T11693	punct	),nM
R7858	T11696	T11674	punct	.,plotted
R7859	T11699	T11700	mark	that,reverse
R786	T1504	T1502	pobj	complex,of
R7860	T11700	T11698	ccomp	reverse,Note
R7861	T11701	T11702	amod	increasing,concentrations
R7862	T11702	T11700	nsubj	concentrations,reverse
R7863	T11703	T11702	prep	of,concentrations
R7864	T11704	T11705	det	the,LD
R7865	T11705	T11703	pobj	LD,of
R7866	T11706	T11705	prep	of,LD
R7867	T11707	T11706	pobj	RanBP2,of
R7868	T11708	T11709	det	the,inhibition
R7869	T11709	T11700	dobj	inhibition,reverse
R787	T1505	T1504	amod	nuclear,complex
R7870	T11710	T11709	prep	of,inhibition
R7871	T11711	T11712	compound	HKI,activity
R7872	T11712	T11710	pobj	activity,of
R7873	T11713	T11709	prep	by,inhibition
R7874	T11714	T11713	pobj	Cox11,by
R7875	T11715	T11698	punct	.,Note
R7876	T11717	T11718	det	A,effect
R7877	T11718	T11722	nsubjpass	effect,observed
R7878	T11719	T11720	advmod	half,maximal
R7879	T11720	T11718	amod	maximal,effect
R788	T1506	T1504	compound	pore,complex
R7880	T11721	T11720	punct	-,maximal
R7881	T11723	T11718	prep	of,effect
R7882	T11724	T11725	det	the,LD
R7883	T11725	T11723	pobj	LD,of
R7884	T11726	T11725	prep	of,LD
R7885	T11727	T11726	pobj	RanBP2,of
R7886	T11728	T11718	prep	on,effect
R7887	T11729	T11730	compound	HKI,activity
R7888	T11730	T11728	pobj	activity,on
R7889	T11731	T11718	prep	in,effect
R789	T1507	T1504	punct	(,complex
R7890	T11732	T11733	det	the,presence
R7891	T11733	T11731	pobj	presence,in
R7892	T11734	T11733	prep	of,presence
R7893	T11735	T11736	nummod	7.5,nM
R7894	T11736	T11734	pobj	nM,of
R7895	T11737	T11736	prep	of,nM
R7896	T11738	T11737	pobj	Cox11,of
R7897	T11739	T11722	auxpass	was,observed
R7898	T11740	T11722	prep	at,observed
R7899	T11741	T11742	det	a,concentration
R79	T318	T316	nsubj	we,report
R790	T1508	T1504	appos	NPC,complex
R7900	T11742	T11740	pobj	concentration,at
R7901	T11743	T11742	prep	of,concentration
R7902	T11744	T11745	punct	~,0.05
R7903	T11745	T11746	nummod	0.05,nM
R7904	T11746	T11743	pobj	nM,of
R7905	T11747	T11746	prep	of,nM
R7906	T11748	T11747	pobj	LD,of
R7907	T11749	T11722	punct	.,observed
R7908	T11751	T11752	punct	(,D
R7909	T11752	T11753	meta	D,plot
R791	T1509	T1471	punct	),supporting
R7910	T11754	T11752	punct	),D
R7911	T11755	T11753	nmod	Rate,plot
R7912	T11756	T11755	cc	versus,Rate
R7913	T11757	T11755	conj	glucose,Rate
R7914	T11758	T11753	prep	in,plot
R7915	T11759	T11760	det	the,absence
R7916	T11760	T11758	pobj	absence,in
R7917	T11761	T11760	cc	and,absence
R7918	T11762	T11760	conj	presence,absence
R7919	T11763	T11760	prep	of,absence
R792	T1510	T1511	punct	[,18
R7920	T11764	T11765	det	the,LD
R7921	T11765	T11763	pobj	LD,of
R7922	T11766	T11765	prep	of,LD
R7923	T11767	T11766	pobj	RanBP2,of
R7924	T11768	T11753	punct	.,plot
R7925	T11770	T11771	prep	At,reduced
R7926	T11772	T11773	det	a,concentration
R7927	T11773	T11770	pobj	concentration,At
R7928	T11774	T11773	compound	saturating,concentration
R7929	T11775	T11773	prep	of,concentration
R793	T1511	T1471	parataxis	18,supporting
R7930	T11776	T11777	det	the,LD
R7931	T11777	T11775	pobj	LD,of
R7932	T11778	T11777	prep	of,LD
R7933	T11779	T11778	pobj	RanBP2,of
R7934	T11780	T11781	punct	(,nM
R7935	T11781	T11773	parataxis	nM,concentration
R7936	T11782	T11781	nummod	3.75,nM
R7937	T11783	T11781	punct	),nM
R7938	T11784	T11771	punct	", ",reduced
R7939	T11785	T11786	det	the,activity
R794	T1512	T1511	punct	],18
R7940	T11786	T11771	nsubjpass	activity,reduced
R7941	T11787	T11786	compound	HKI,activity
R7942	T11788	T11771	auxpass	was,reduced
R7943	T11789	T11771	prep	by,reduced
R7944	T11790	T11791	quantmod	about,20
R7945	T11791	T11792	nummod	20,%
R7946	T11792	T11789	pobj	%,by
R7947	T11793	T11771	punct	.,reduced
R7948	T11796	T11795	punct	", ",v
R7949	T11797	T11795	appos	rate,v
R795	T1513	T1457	punct	.,found
R7950	T11798	T11795	punct	;,v
R7951	T11799	T11795	appos	S,v
R7952	T11800	T11799	punct	", ",S
R7953	T11801	T11799	appos	glucose,S
R7954	T11802	T11795	punct	.,v
R7957	T11946	T11945	prep	of,Localization
R7958	T11947	T11946	pobj	RanBP2,of
R7959	T11948	T11947	cc	and,RanBP2
R796	T1515	T1516	advmod	Finally,found
R7960	T11949	T11950	poss	Its,Partners
R7961	T11950	T11947	conj	Partners,RanBP2
R7962	T11951	T11950	nmod	LD,Partners
R7963	T11952	T11950	amod	Molecular,Partners
R7964	T11954	T11955	punct	(,are
R7965	T11956	T11955	nsubj	A,are
R7966	T11957	T11958	punct	–,F
R7967	T11958	T11956	prep	F,A
R7968	T11959	T11955	punct	),are
R7969	T11960	T11961	amod	thin,cryosections
R797	T1517	T1516	punct	", ",found
R7970	T11961	T11955	attr	cryosections,are
R7971	T11962	T11961	prep	of,cryosections
R7972	T11963	T11964	det	an,area
R7973	T11964	T11962	pobj	area,of
R7974	T11965	T11964	prep	of,area
R7975	T11966	T11967	det	the,hipocampus
R7976	T11967	T11965	pobj	hipocampus,of
R7977	T11968	T11969	punct	(,neurons
R7978	T11969	T11967	parataxis	neurons,hipocampus
R7979	T11970	T11969	compound	CA1,neurons
R798	T1518	T1516	nsubjpass	RanBP2,found
R7980	T11971	T11969	punct	),neurons
R7981	T11972	T11967	cc	and,hipocampus
R7982	T11973	T11974	amod	cerebral,cortex
R7983	T11974	T11967	conj	cortex,hipocampus
R7984	T11975	T11967	punct	", ",hipocampus
R7985	T11976	T11967	advmod	respectively,hipocampus
R7986	T11977	T11961	punct	", ",cryosections
R7987	T11978	T11961	acl	immunostained,cryosections
R7988	T11979	T11978	prep	against,immunostained
R7989	T11980	T11979	pobj	HKI,against
R799	T1519	T1516	auxpass	was,found
R7990	T11981	T11982	punct	(,A
R7991	T11982	T11980	parataxis	A,HKI
R7992	T11983	T11982	cc	and,A
R7993	T11984	T11982	conj	D,A
R7994	T11985	T11982	punct	),A
R7995	T11986	T11980	punct	", ",HKI
R7996	T11987	T11980	conj	RanBP2,HKI
R7997	T11988	T11989	punct	(,B
R7998	T11989	T11987	parataxis	B,RanBP2
R7999	T11990	T11989	cc	and,B
R8	T237	T234	conj	Hexokinase,Cox11
R80	T319	T320	det	the,identification
R800	T1520	T1516	advmod	also,found
R8000	T11991	T11989	conj	E,B
R8001	T11992	T11989	punct	),B
R8002	T11993	T11987	punct	", ",RanBP2
R8003	T11994	T11987	cc	and,RanBP2
R8004	T11995	T11996	amod	merged,images
R8005	T11996	T11987	conj	images,RanBP2
R8006	T11997	T11996	advmod	thereof,images
R8007	T11998	T11999	punct	(,C
R8008	T11999	T11996	parataxis	C,images
R8009	T12000	T11999	cc	and,C
R801	T1521	T1522	aux	to,localize
R8010	T12001	T11999	conj	F,C
R8011	T12002	T11999	punct	),C
R8012	T12003	T11955	punct	.,are
R8013	T12006	T12007	mark	that,restricted
R8014	T12007	T12005	ccomp	restricted,Note
R8015	T12008	T12009	mark	while,expressed
R8016	T12009	T12007	advcl	expressed,restricted
R8017	T12010	T12009	nsubjpass	RanBP2,expressed
R8018	T12011	T12010	cc	and,RanBP2
R8019	T12012	T12010	conj	HKI,RanBP2
R802	T1522	T1516	xcomp	localize,found
R8020	T12013	T12009	auxpass	are,expressed
R8021	T12014	T12009	advmod	widely,expressed
R8022	T12015	T12009	prep	among,expressed
R8023	T12016	T12009	cc	and,expressed
R8024	T12017	T12009	conj	colocalize,expressed
R8025	T12018	T12017	prep	to,colocalize
R8026	T12019	T12020	amod	hippocampal,neurons
R8027	T12020	T12017	dobj	neurons,colocalize
R8028	T12021	T12022	punct	(,C
R8029	T12022	T12017	parataxis	C,colocalize
R803	T1523	T1522	advmod	prominently,localize
R8030	T12023	T12022	punct	),C
R8031	T12024	T12007	punct	", ",restricted
R8032	T12025	T12026	compound	HKI,expression
R8033	T12026	T12007	nsubjpass	expression,restricted
R8034	T12027	T12026	cc	and,expression
R8035	T12028	T12026	conj	localization,expression
R8036	T12029	T12026	prep	with,expression
R8037	T12030	T12029	pobj	RanBP2,with
R8038	T12031	T12007	auxpass	is,restricted
R8039	T12032	T12007	prep	to,restricted
R804	T1524	T1522	prep	to,localize
R8040	T12033	T12034	det	a,subset
R8041	T12034	T12032	pobj	subset,to
R8042	T12035	T12034	prep	of,subset
R8043	T12036	T12037	amod	cortical,neurons
R8044	T12037	T12035	pobj	neurons,of
R8045	T12038	T12034	punct	(,subset
R8046	T12039	T12040	amod	likely,interneurons
R8047	T12040	T12034	appos	interneurons,subset
R8048	T12041	T12007	punct	),restricted
R8049	T12042	T12043	punct	(,F
R805	T1525	T1526	det	the,compartment
R8050	T12043	T12007	parataxis	F,restricted
R8051	T12044	T12043	punct	),F
R8052	T12045	T12005	punct	.,Note
R8053	T12047	T12048	nsubjpass	Images,immunostained
R8054	T12049	T12047	prep	of,Images
R8055	T12050	T12051	det	the,region
R8056	T12051	T12049	pobj	region,of
R8057	T12052	T12051	amod	distal,region
R8058	T12053	T12051	prep	of,region
R8059	T12054	T12055	amod	bovine,cryosections
R806	T1526	T1524	pobj	compartment,to
R8060	T12055	T12053	pobj	cryosections,of
R8061	T12056	T12055	amod	retinal,cryosections
R8062	T12057	T12055	acl	comprising,cryosections
R8063	T12058	T12057	dobj	part,comprising
R8064	T12059	T12058	prep	of,part
R8065	T12060	T12061	det	the,layer
R8066	T12061	T12059	pobj	layer,of
R8067	T12062	T12061	amod	nuclear,layer
R8068	T12063	T12061	prep	of,layer
R8069	T12064	T12065	compound	photoreceptor,neurons
R807	T1527	T1528	npadvmod	mitochondria,rich
R8070	T12065	T12063	pobj	neurons,of
R8071	T12066	T12065	cc	and,neurons
R8072	T12067	T12068	poss	their,compartment
R8073	T12068	T12065	conj	compartment,neurons
R8074	T12069	T12068	amod	inner,compartment
R8075	T12070	T12068	punct	(,compartment
R8076	T12071	T12068	amod	myoid,compartment
R8077	T12072	T12071	cc	and,myoid
R8078	T12073	T12071	conj	ellipsoid,myoid
R8079	T12074	T12068	punct	),compartment
R808	T1528	T1526	amod	rich,compartment
R8080	T12075	T12068	compound	segment,compartment
R8081	T12076	T12077	punct	(,G
R8082	T12077	T12057	parataxis	G,comprising
R8083	T12078	T12079	punct	–,O
R8084	T12079	T12077	prep	O,G
R8085	T12080	T12077	punct	),G
R8086	T12081	T12048	auxpass	are,immunostained
R8087	T12082	T12048	prep	against,immunostained
R8088	T12083	T12082	pobj	mHsp70,against
R8089	T12084	T12085	punct	(,G
R809	T1529	T1528	punct	-,rich
R8090	T12085	T12083	parataxis	G,mHsp70
R8091	T12086	T12085	punct	),G
R8092	T12087	T12083	cc	and,mHsp70
R8093	T12088	T12083	conj	RanBP2,mHsp70
R8094	T12089	T12090	punct	(,H
R8095	T12090	T12088	parataxis	H,RanBP2
R8096	T12091	T12090	punct	),H
R8097	T12092	T12088	punct	", ",RanBP2
R8098	T12093	T12088	conj	mHsp70,RanBP2
R8099	T12094	T12095	punct	(,J
R81	T320	T316	dobj	identification,report
R810	T1530	T1526	amod	ellipsoid,compartment
R8100	T12095	T12093	parataxis	J,mHsp70
R8101	T12096	T12095	punct	),J
R8102	T12097	T12093	cc	and,mHsp70
R8103	T12098	T12093	conj	Cox11,mHsp70
R8104	T12099	T12100	punct	(,K
R8105	T12100	T12098	parataxis	K,Cox11
R8106	T12101	T12100	punct	),K
R8107	T12102	T12098	punct	", ",Cox11
R8108	T12103	T12098	conj	HKI,Cox11
R8109	T12104	T12105	punct	(,M
R811	T1531	T1526	amod	subcellular,compartment
R8110	T12105	T12103	parataxis	M,HKI
R8111	T12106	T12105	punct	),M
R8112	T12107	T12103	cc	and,HKI
R8113	T12108	T12103	conj	Cox11,HKI
R8114	T12109	T12110	punct	(,N
R8115	T12110	T12108	parataxis	N,Cox11
R8116	T12111	T12110	punct	),N
R8117	T12112	T12108	punct	", ",Cox11
R8118	T12113	T12108	cc	and,Cox11
R8119	T12114	T12115	amod	merged,images
R812	T1532	T1526	prep	of,compartment
R8120	T12115	T12108	conj	images,Cox11
R8121	T12116	T12115	advmod	thereof,images
R8122	T12117	T12118	punct	(,I
R8123	T12118	T12048	parataxis	I,immunostained
R8124	T12119	T12120	punct	–,O
R8125	T12120	T12118	prep	O,I
R8126	T12121	T12118	punct	),I
R8127	T12122	T12048	punct	.,immunostained
R8128	T12125	T12126	det	the,localization
R8129	T12126	T12124	dobj	localization,Note
R813	T1533	T1534	amod	photosensory,neurons
R8130	T12127	T12126	amod	prominent,localization
R8131	T12128	T12126	prep	of,localization
R8132	T12129	T12128	pobj	RanBP2,of
R8133	T12130	T12129	punct	", ",RanBP2
R8134	T12131	T12129	conj	mHsp70,RanBP2
R8135	T12132	T12131	punct	", ",mHsp70
R8136	T12133	T12131	cc	and,mHsp70
R8137	T12134	T12131	conj	Cox11,mHsp70
R8138	T12135	T12126	prep	at,localization
R8139	T12136	T12137	det	the,compartment
R814	T1534	T1532	pobj	neurons,of
R8140	T12137	T12135	pobj	compartment,at
R8141	T12138	T12139	npadvmod	mitochondria,rich
R8142	T12139	T12137	amod	rich,compartment
R8143	T12140	T12139	punct	-,rich
R8144	T12141	T12137	amod	ellipsoid,compartment
R8145	T12142	T12137	prep	of,compartment
R8146	T12143	T12142	pobj	photoreceptors,of
R8147	T12144	T12126	cc	and,localization
R8148	T12145	T12146	det	the,colocalization
R8149	T12146	T12126	conj	colocalization,localization
R815	T1535	T1536	punct	[,19
R8150	T12147	T12146	prep	of,colocalization
R8151	T12148	T12147	pobj	RanBP2,of
R8152	T12149	T12148	cc	and,RanBP2
R8153	T12150	T12148	conj	Cox11,RanBP2
R8154	T12151	T12146	prep	with,colocalization
R8155	T12152	T12151	pobj	mHsp70,with
R8156	T12153	T12154	punct	(,I
R8157	T12154	T12146	parataxis	I,colocalization
R8158	T12155	T12154	cc	and,I
R8159	T12156	T12154	conj	L,I
R816	T1536	T1526	parataxis	19,compartment
R8160	T12157	T12154	punct	),I
R8161	T12158	T12124	punct	", ",Note
R8162	T12159	T12160	mark	while,limited
R8163	T12160	T12124	advcl	limited,Note
R8164	T12161	T12162	compound	HKI,colocalization
R8165	T12162	T12160	nsubjpass	colocalization,limited
R8166	T12163	T12162	prep	with,colocalization
R8167	T12164	T12163	pobj	Cox11,with
R8168	T12165	T12160	auxpass	was,limited
R8169	T12166	T12160	prep	to,limited
R817	T1537	T1536	punct	],19
R8170	T12167	T12168	amod	restricted,foci
R8171	T12168	T12166	pobj	foci,to
R8172	T12169	T12170	punct	(,arrowheads
R8173	T12170	T12160	parataxis	arrowheads,limited
R8174	T12171	T12170	dep	R,arrowheads
R8175	T12172	T12170	punct	", ",arrowheads
R8176	T12173	T12170	punct	),arrowheads
R8177	T12174	T12124	punct	.,Note
R8178	T12176	T12177	amod	High,resolution
R8179	T12177	T12179	compound	resolution,images
R818	T1538	T1524	cc	and,to
R8180	T12178	T12177	punct	-,resolution
R8181	T12179	T12180	nsubj	images,confirmed
R8182	T12181	T12179	prep	of,images
R8183	T12182	T12183	amod	dissociated,neurons
R8184	T12183	T12181	pobj	neurons,of
R8185	T12184	T12183	amod	primary,neurons
R8186	T12185	T12183	amod	cerebral,neurons
R8187	T12186	T12183	cc	and,neurons
R8188	T12187	T12188	amod	glial,cells
R8189	T12188	T12183	conj	cells,neurons
R819	T1539	T1524	conj	to,to
R8190	T12189	T12190	mark	that,was
R8191	T12190	T12180	ccomp	was,confirmed
R8192	T12191	T12192	det	the,colocalization
R8193	T12192	T12190	nsubj	colocalization,was
R8194	T12193	T12192	prep	of,colocalization
R8195	T12194	T12193	pobj	HKI,of
R8196	T12195	T12194	cc	and,HKI
R8197	T12196	T12194	conj	Cox11,HKI
R8198	T12197	T12198	advmod	highly,restricted
R8199	T12198	T12190	acomp	restricted,was
R82	T321	T320	prep	of,identification
R820	T1540	T1541	npadvmod	RanGTPase,restricted
R8200	T12199	T12200	punct	(,P
R8201	T12200	T12198	parataxis	P,restricted
R8202	T12201	T12202	punct	–,R
R8203	T12202	T12200	prep	R,P
R8204	T12203	T12200	punct	),P
R8205	T12204	T12190	punct	", ",was
R8206	T12205	T12206	mark	while,colocalized
R8207	T12206	T12190	advcl	colocalized,was
R8208	T12207	T12206	nsubj	RanBP2,colocalized
R8209	T12208	T12206	advmod	extensively,colocalized
R821	T1541	T1543	amod	restricted,foci
R8210	T12209	T12206	prep	with,colocalized
R8211	T12210	T12209	pobj	HKI,with
R8212	T12211	T12212	punct	(,S
R8213	T12212	T12210	parataxis	S,HKI
R8214	T12213	T12214	punct	–,U
R8215	T12214	T12212	prep	U,S
R8216	T12215	T12212	punct	),S
R8217	T12216	T12210	cc	and,HKI
R8218	T12217	T12210	conj	mHsp70,HKI
R8219	T12218	T12219	punct	(,V
R822	T1542	T1541	punct	-,restricted
R8220	T12219	T12217	parataxis	V,mHsp70
R8221	T12220	T12221	punct	–,Z
R8222	T12221	T12219	prep	Z,V
R8223	T12222	T12219	punct	),V
R8224	T12223	T12180	punct	.,confirmed
R8225	T12225	T12226	compound	Scale,bars
R8226	T12226	T12227	nsubj	bars,are
R8227	T12228	T12226	prep	in,bars
R8228	T12229	T12228	pobj	A,in
R8229	T12230	T12231	punct	–,O
R823	T1543	T1539	pobj	foci,to
R8230	T12231	T12229	prep	O,A
R8231	T12232	T12229	cc	and,A
R8232	T12233	T12229	conj	P,A
R8233	T12234	T12235	punct	–,Z
R8234	T12235	T12233	prep	Z,P
R8235	T12236	T12237	nummod	40,μm
R8236	T12237	T12227	attr	μm,are
R8237	T12238	T12236	cc	and,40
R8238	T12239	T12236	conj	10,40
R8239	T12240	T12227	punct	", ",are
R824	T1544	T1543	prep	along,foci
R8240	T12241	T12227	advmod	respectively,are
R8241	T12242	T12227	punct	.,are
R8242	T12245	T12244	punct	", ",ONL
R8243	T12246	T12247	amod	outer,layer
R8244	T12247	T12244	appos	layer,ONL
R8245	T12248	T12247	amod	nuclear,layer
R8246	T12249	T12244	punct	.,ONL
R825	T1545	T1546	amod	cytoplasmic,tracks
R8251	T12535	T12536	compound	Insertion,Mutagenesis
R8252	T12537	T12536	prep	of,Mutagenesis
R8253	T12538	T12539	det	the,Gene
R8254	T12539	T12537	pobj	Gene,of
R8255	T12540	T12539	amod	Murine,Gene
R8256	T12541	T12539	compound	RanBP2,Gene
R8257	T12543	T12544	punct	(,A
R8258	T12544	T12545	meta	A,Diagram
R8259	T12546	T12544	punct	),A
R826	T1546	T1544	pobj	tracks,along
R8260	T12547	T12545	prep	of,Diagram
R8261	T12548	T12549	det	the,region
R8262	T12549	T12547	pobj	region,of
R8263	T12550	T12549	amod	genomic,region
R8264	T12551	T12549	prep	of,region
R8265	T12552	T12551	pobj	RanBP2,of
R8266	T12553	T12549	acl	disrupted,region
R8267	T12554	T12553	agent	by,disrupted
R8268	T12555	T12556	compound	insertion,trap
R8269	T12556	T12557	compound	trap,mutagenesis
R827	T1547	T1548	punct	[,19
R8270	T12557	T12554	pobj	mutagenesis,by
R8271	T12558	T12557	prep	with,mutagenesis
R8272	T12559	T12560	det	a,vector
R8273	T12560	T12558	pobj	vector,with
R8274	T12561	T12560	amod	bicistronic,vector
R8275	T12562	T12560	compound	reporter,vector
R8276	T12563	T12560	prep	between,vector
R8277	T12564	T12565	nmod	exon,1
R8278	T12565	T12563	pobj	1,between
R8279	T12566	T12565	cc	and,1
R828	T1548	T1546	parataxis	19,tracks
R8280	T12567	T12565	conj	2,1
R8281	T12568	T12545	punct	.,Diagram
R8282	T12570	T12571	det	The,transcript
R8283	T12571	T12573	nsubj	transcript,produces
R8284	T12572	T12571	amod	bicistronic,transcript
R8285	T12574	T12575	nummod	two,proteins
R8286	T12575	T12573	dobj	proteins,produces
R8287	T12576	T12573	prep	under,produces
R8288	T12577	T12576	pobj	regulation,under
R8289	T12578	T12577	prep	of,regulation
R829	T1549	T1548	punct	],19
R8290	T12579	T12578	pobj	RanBP2,of
R8291	T12580	T12573	punct	.,produces
R8292	T12582	T12583	prep	Upon,generated
R8293	T12584	T12582	pobj	splicing,Upon
R8294	T12585	T12584	prep	of,splicing
R8295	T12586	T12585	pobj	RanBP2,of
R8296	T12587	T12583	punct	", ",generated
R8297	T12588	T12589	det	a,fusion
R8298	T12589	T12583	nsubjpass	fusion,generated
R8299	T12590	T12589	prep	between,fusion
R83	T322	T323	nummod	two,partners
R830	T1550	T1522	punct	", ",localize
R8300	T12591	T12590	pobj	exon,between
R8301	T12592	T12591	nummod	1,exon
R8302	T12593	T12591	cc	and,exon
R8303	T12594	T12595	compound	β,geo
R8304	T12595	T12591	conj	geo,exon
R8305	T12596	T12595	punct	-,geo
R8306	T12597	T12595	punct	(,geo
R8307	T12598	T12599	det	a,fusion
R8308	T12599	T12595	appos	fusion,geo
R8309	T12600	T12599	prep	between,fusion
R831	T1551	T1522	prep	in,localize
R8310	T12601	T12602	det	the,genes
R8311	T12602	T12600	pobj	genes,between
R8312	T12603	T12604	nmod	β,gal
R8313	T12604	T12602	nmod	gal,genes
R8314	T12605	T12604	punct	-,gal
R8315	T12606	T12604	cc	and,gal
R8316	T12607	T12604	conj	neo,gal
R8317	T12608	T12583	punct	),generated
R8318	T12609	T12583	auxpass	is,generated
R8319	T12610	T12583	punct	", ",generated
R832	T1552	T1551	pobj	addition,in
R8320	T12611	T12612	mark	while,translated
R8321	T12612	T12583	advcl	translated,generated
R8322	T12613	T12614	amod	human,phophatase
R8323	T12614	T12612	nsubjpass	phophatase,translated
R8324	T12615	T12614	amod	placental,phophatase
R8325	T12616	T12614	compound	alkaline,phophatase
R8326	T12617	T12614	punct	(,phophatase
R8327	T12618	T12614	appos	PLAP,phophatase
R8328	T12619	T12612	punct	),translated
R8329	T12620	T12612	auxpass	is,translated
R833	T1553	T1552	prep	to,addition
R8330	T12621	T12612	advmod	independently,translated
R8331	T12622	T12612	advcl	using,translated
R8332	T12623	T12624	det	the,site
R8333	T12624	T12622	dobj	site,using
R8334	T12625	T12626	amod	internal,entry
R8335	T12626	T12624	compound	entry,site
R8336	T12627	T12626	compound	ribosome,entry
R8337	T12628	T12583	punct	.,generated
R8338	T12630	T12631	advcl	Consistent,directed
R8339	T12632	T12630	prep	with,Consistent
R834	T1554	T1555	poss	its,localization
R8340	T12633	T12634	amod	previous,studies
R8341	T12634	T12632	pobj	studies,with
R8342	T12635	T12631	punct	", ",directed
R8343	T12636	T12637	det	the,expression
R8344	T12637	T12631	nsubjpass	expression,directed
R8345	T12638	T12637	prep	of,expression
R8346	T12639	T12640	det	the,former
R8347	T12640	T12638	pobj	former,of
R8348	T12641	T12631	auxpass	is,directed
R8349	T12642	T12631	prep	to,directed
R835	T1555	T1553	pobj	localization,to
R8350	T12643	T12644	compound	cell,bodies
R8351	T12644	T12642	pobj	bodies,to
R8352	T12645	T12631	punct	", ",directed
R8353	T12646	T12647	mark	while,targeted
R8354	T12647	T12631	advcl	targeted,directed
R8355	T12648	T12647	nsubjpass	expression,targeted
R8356	T12649	T12648	prep	of,expression
R8357	T12650	T12651	det	the,latter
R8358	T12651	T12649	pobj	latter,of
R8359	T12652	T12647	auxpass	is,targeted
R836	T1556	T1555	prep	at,localization
R8360	T12653	T12647	prep	to,targeted
R8361	T12654	T12655	det	the,processes
R8362	T12655	T12653	pobj	processes,to
R8363	T12656	T12655	amod	axonal,processes
R8364	T12657	T12658	punct	[,68
R8365	T12658	T12647	parataxis	68,targeted
R8366	T12659	T12658	nummod	67,68
R8367	T12660	T12658	punct	",",68
R8368	T12661	T12658	punct	],68
R8369	T12662	T12631	punct	.,directed
R837	T1557	T1558	amod	cytoplasmic,fibrils
R8370	T12664	T12665	amod	Transcriptional,analysis
R8371	T12665	T12669	nsubj	analysis,detects
R8372	T12666	T12665	nummod	5,analysis
R8373	T12667	T12666	punct	′,5
R8374	T12668	T12665	compound	RACE,analysis
R8375	T12670	T12671	det	a,fusion
R8376	T12671	T12669	dobj	fusion,detects
R8377	T12672	T12671	prep	between,fusion
R8378	T12673	T12672	pobj	exon,between
R8379	T12674	T12673	nummod	1,exon
R838	T1558	T1556	pobj	fibrils,at
R8380	T12675	T12673	cc	and,exon
R8381	T12676	T12677	compound	β,geo
R8382	T12677	T12673	conj	geo,exon
R8383	T12678	T12677	punct	-,geo
R8384	T12679	T12669	punct	.,detects
R8385	T12681	T12682	punct	(,B
R8386	T12682	T12683	meta	B,analysis
R8387	T12684	T12682	punct	),B
R8388	T12685	T12683	compound	Southern,analysis
R8389	T12686	T12683	prep	of,analysis
R839	T1559	T1558	acl	emanating,fibrils
R8390	T12687	T12688	det	the,locus
R8391	T12688	T12686	pobj	locus,of
R8392	T12689	T12688	compound	RanBP2,locus
R8393	T12690	T12688	prep	of,locus
R8394	T12691	T12692	amod	wild,type
R8395	T12692	T12694	nmod	type,DNA
R8396	T12693	T12692	punct	-,type
R8397	T12694	T12690	pobj	DNA,of
R8398	T12695	T12692	cc	and,type
R8399	T12696	T12692	conj	heterozygous,type
R84	T323	T321	pobj	partners,of
R840	T1560	T1559	prep	from,emanating
R8400	T12697	T12694	amod	genomic,DNA
R8401	T12698	T12694	prep	of,DNA
R8402	T12699	T12698	pobj	tails,of
R8403	T12700	T12699	prep	of,tails
R8404	T12701	T12702	compound	F1,mice
R8405	T12702	T12700	pobj	mice,of
R8406	T12703	T12702	acl	digested,mice
R8407	T12704	T12703	prep	with,digested
R8408	T12705	T12704	pobj	PpuMI,with
R8409	T12706	T12707	punct	(,panel
R841	T1561	T1562	amod	nuclear,pores
R8410	T12707	T12705	parataxis	panel,PpuMI
R8411	T12708	T12707	amod	left,panel
R8412	T12709	T12707	punct	),panel
R8413	T12710	T12705	cc	and,PpuMI
R8414	T12711	T12705	conj	HindIII,PpuMI
R8415	T12712	T12713	punct	(,panel
R8416	T12713	T12711	parataxis	panel,HindIII
R8417	T12714	T12713	amod	right,panel
R8418	T12715	T12713	punct	),panel
R8419	T12716	T12703	prep	with,digested
R842	T1562	T1560	pobj	pores,from
R8420	T12717	T12716	pobj	probes,with
R8421	T12718	T12717	prep	at,probes
R8422	T12719	T12720	det	the,regions
R8423	T12720	T12718	pobj	regions,at
R8424	T12721	T12720	nummod	3,regions
R8425	T12722	T12721	punct	′,3
R8426	T12723	T12724	punct	(,panel
R8427	T12724	T12721	parataxis	panel,3
R8428	T12725	T12724	amod	left,panel
R8429	T12726	T12724	punct	),panel
R843	T1563	T1564	punct	[,19
R8430	T12727	T12721	cc	and,3
R8431	T12728	T12721	conj	5,3
R8432	T12729	T12728	punct	′,5
R8433	T12730	T12731	punct	(,panel
R8434	T12731	T12728	parataxis	panel,5
R8435	T12732	T12731	amod	right,panel
R8436	T12733	T12731	punct	),panel
R8437	T12734	T12720	compound	flanking,regions
R8438	T12735	T12720	prep	of,regions
R8439	T12736	T12737	det	the,breakpoint
R844	T1564	T1522	parataxis	19,localize
R8440	T12737	T12735	pobj	breakpoint,of
R8441	T12738	T12737	compound	insertion,breakpoint
R8442	T12739	T12683	punct	.,analysis
R8443	T12741	T12742	nsubj	Q1,is
R8444	T12743	T12744	det	a,cosmid
R8445	T12744	T12742	attr	cosmid,is
R8446	T12745	T12744	acl	containing,cosmid
R8447	T12746	T12747	det	the,gene
R8448	T12747	T12745	dobj	gene,containing
R8449	T12748	T12747	compound	RanBP2,gene
R845	T1565	T1564	nummod	2,19
R8450	T12749	T12745	prep	up,containing
R8451	T12750	T12749	prep	to,up
R8452	T12751	T12750	pobj	exon,to
R8453	T12752	T12751	nummod	20,exon
R8454	T12753	T12754	punct	[,4
R8455	T12754	T12745	parataxis	4,containing
R8456	T12755	T12754	punct	],4
R8457	T12756	T12742	punct	.,is
R8458	T12758	T12759	punct	(,C
R8459	T12759	T12760	meta	C,view
R846	T1566	T1564	punct	",",19
R8460	T12761	T12759	punct	),C
R8461	T12762	T12760	amod	Lateroventral,view
R8462	T12763	T12760	prep	of,view
R8463	T12764	T12765	det	a,stain
R8464	T12765	T12763	pobj	stain,of
R8465	T12766	T12767	amod	whole,mount
R8466	T12767	T12765	compound	mount,stain
R8467	T12768	T12767	punct	-,mount
R8468	T12769	T12765	prep	of,stain
R8469	T12770	T12771	det	a,embryo
R847	T1567	T1564	nummod	3,19
R8470	T12771	T12769	pobj	embryo,of
R8471	T12772	T12773	punct	~,12.5
R8472	T12773	T12774	nummod	12.5,dpc
R8473	T12774	T12771	nmod	dpc,embryo
R8474	T12775	T12771	amod	heterozygous,embryo
R8475	T12776	T12765	prep	for,stain
R8476	T12777	T12778	nmod	PLAP,activities
R8477	T12778	T12776	pobj	activities,for
R8478	T12779	T12777	cc	and,PLAP
R8479	T12780	T12781	compound	β,gal
R848	T1568	T1564	punct	",",19
R8480	T12781	T12777	conj	gal,PLAP
R8481	T12782	T12781	punct	-,gal
R8482	T12783	T12784	punct	(,picture
R8483	T12784	T12781	parataxis	picture,gal
R8484	T12785	T12784	compound	inset,picture
R8485	T12786	T12784	punct	),picture
R8486	T12787	T12760	punct	.,view
R8487	T12789	T12790	mark	Although,expressed
R8488	T12790	T12794	advcl	expressed,was
R8489	T12791	T12790	nsubjpass	PLAP,expressed
R849	T1569	T1564	nummod	10,19
R8490	T12792	T12790	auxpass	was,expressed
R8491	T12793	T12790	advmod	broadly,expressed
R8492	T12795	T12790	punct	(,expressed
R8493	T12796	T12797	advmod	e.g.,somites
R8494	T12797	T12790	npadvmod	somites,expressed
R8495	T12798	T12797	punct	", ",somites
R8496	T12799	T12797	punct	", ",somites
R8497	T12800	T12797	conj	limbs,somites
R8498	T12801	T12800	punct	", ",limbs
R8499	T12802	T12800	cc	and,limbs
R85	T324	T323	amod	novel,partners
R850	T1570	T1564	punct	",",19
R8500	T12803	T12800	conj	CNS,limbs
R8501	T12804	T12794	punct	),was
R8502	T12805	T12794	punct	", ",was
R8503	T12806	T12807	det	the,expression
R8504	T12807	T12794	nsubj	expression,was
R8505	T12808	T12807	nmod	PLAP,expression
R8506	T12809	T12808	cc	and,PLAP
R8507	T12810	T12811	compound	β,Gal
R8508	T12811	T12808	conj	Gal,PLAP
R8509	T12812	T12811	punct	-,Gal
R851	T1571	T1564	nummod	12,19
R8510	T12813	T12814	punct	(,picture
R8511	T12814	T12811	parataxis	picture,Gal
R8512	T12815	T12814	compound	inset,picture
R8513	T12816	T12814	punct	),picture
R8514	T12817	T12818	advmod	particularly,high
R8515	T12818	T12794	acomp	high,was
R8516	T12819	T12794	prep	in,was
R8517	T12820	T12821	det	the,vesicle
R8518	T12821	T12819	pobj	vesicle,in
R8519	T12822	T12821	amod	optic,vesicle
R852	T1572	T1564	punct	",",19
R8520	T12823	T12824	punct	(,arrow
R8521	T12824	T12794	parataxis	arrow,was
R8522	T12825	T12824	punct	),arrow
R8523	T12826	T12794	punct	.,was
R8524	T12828	T12829	nmod	X,gal
R8525	T12829	T12831	nmod	gal,staining
R8526	T12830	T12829	punct	-,gal
R8527	T12832	T12831	amod	single,staining
R8528	T12833	T12834	punct	(,D
R8529	T12834	T12832	parataxis	D,single
R853	T1573	T1564	punct	],19
R8530	T12835	T12834	punct	),D
R8531	T12836	T12832	cc	and,single
R8532	T12837	T12832	conj	combined,single
R8533	T12838	T12831	prep	with,staining
R8534	T12839	T12838	pobj	PLAP,with
R8535	T12840	T12841	punct	(,E
R8536	T12841	T12839	parataxis	E,PLAP
R8537	T12842	T12841	punct	),E
R8538	T12843	T12831	prep	of,staining
R8539	T12844	T12845	det	a,section
R854	T1574	T1516	punct	.,found
R8540	T12845	T12843	pobj	section,of
R8541	T12846	T12845	amod	retinal,section
R8542	T12847	T12845	prep	of,section
R8543	T12848	T12849	det	a,mouse
R8544	T12849	T12847	pobj	mouse,of
R8545	T12850	T12851	nummod	3,mo
R8546	T12851	T12853	npadvmod	mo,old
R8547	T12852	T12851	punct	-,mo
R8548	T12853	T12849	amod	old,mouse
R8549	T12854	T12853	punct	-,old
R855	T1576	T1577	amod	Emerging,evidence
R8550	T12855	T12849	nmod	RanBP2,mouse
R8551	T12856	T12855	punct	+,RanBP2
R8552	T12857	T12855	punct	/,RanBP2
R8553	T12858	T12855	punct	−,RanBP2
R8554	T12859	T12831	punct	.,staining
R8555	T12861	T12862	advcl	Consistent,detected
R8556	T12863	T12861	prep	with,Consistent
R8557	T12864	T12865	amod	previous,studies
R8558	T12865	T12863	pobj	studies,with
R8559	T12866	T12865	compound	immunocytochemistry,studies
R856	T1577	T1578	nsubj	evidence,supports
R8560	T12867	T12862	punct	", ",detected
R8561	T12868	T12869	compound	β,Gal
R8562	T12869	T12871	compound	Gal,activity
R8563	T12870	T12869	punct	-,Gal
R8564	T12871	T12862	nsubjpass	activity,detected
R8565	T12872	T12862	auxpass	is,detected
R8566	T12873	T12862	prep	in,detected
R8567	T12874	T12875	det	the,bodies
R8568	T12875	T12873	pobj	bodies,in
R8569	T12876	T12875	amod	neuroretinal,bodies
R857	T1579	T1580	mark	that,underlie
R8570	T12877	T12875	cc	and,bodies
R8571	T12878	T12879	amod	inner,compartment
R8572	T12879	T12875	conj	compartment,bodies
R8573	T12880	T12879	compound	segment,compartment
R8574	T12881	T12875	prep	of,bodies
R8575	T12882	T12881	pobj	photoreceptors,of
R8576	T12883	T12882	prep	with,photoreceptors
R8577	T12884	T12885	advmod	conspicuously,strong
R8578	T12885	T12886	amod	strong,expression
R8579	T12886	T12883	pobj	expression,with
R858	T1580	T1578	ccomp	underlie,supports
R8580	T12887	T12886	prep	in,expression
R8581	T12888	T12889	compound	ganglion,cells
R8582	T12889	T12887	pobj	cells,in
R8583	T12890	T12862	punct	.,detected
R8584	T12892	T12893	compound	PLAP,expression
R8585	T12893	T12894	nsubjpass	expression,found
R8586	T12895	T12894	auxpass	is,found
R8587	T12896	T12894	prep	throughout,found
R8588	T12897	T12898	det	the,layers
R8589	T12898	T12896	pobj	layers,throughout
R859	T1581	T1582	det	the,effects
R8590	T12899	T12900	amod	plexiform,synaptic
R8591	T12900	T12898	amod	synaptic,layers
R8592	T12901	T12900	punct	/,synaptic
R8593	T12902	T12898	cc	and,layers
R8594	T12903	T12904	amod	outer,segment
R8595	T12904	T12898	conj	segment,layers
R8596	T12905	T12904	prep	of,segment
R8597	T12906	T12905	pobj	photoreceptors,of
R8598	T12907	T12908	punct	(,E
R8599	T12908	T12894	parataxis	E,found
R86	T325	T323	prep	of,partners
R860	T1582	T1580	nsubj	effects,underlie
R8600	T12909	T12908	punct	),E
R8601	T12910	T12894	punct	.,found
R8602	T12913	T12912	punct	", ",GC
R8603	T12914	T12915	compound	ganglion,cell
R8604	T12915	T12912	appos	cell,GC
R8605	T12916	T12912	punct	;,GC
R8606	T12917	T12912	appos	PLAP,GC
R8607	T12918	T12917	punct	", ",PLAP
R8608	T12919	T12920	amod	human,phophatase
R8609	T12920	T12917	appos	phophatase,PLAP
R861	T1583	T1584	npadvmod	CNS,selective
R8610	T12921	T12920	amod	placental,phophatase
R8611	T12922	T12920	compound	alkaline,phophatase
R8612	T12923	T12912	punct	;,GC
R8613	T12924	T12912	appos	ROS,GC
R8614	T12925	T12924	punct	", ",ROS
R8615	T12926	T12927	nmod	rod,segment
R8616	T12927	T12924	appos	segment,ROS
R8617	T12928	T12927	amod	outer,segment
R8618	T12929	T12912	punct	;,GC
R8619	T12930	T12912	appos	RIS,GC
R862	T1584	T1582	amod	selective,effects
R8620	T12931	T12930	punct	", ",RIS
R8621	T12932	T12933	nmod	rod,segment
R8622	T12933	T12930	appos	segment,RIS
R8623	T12934	T12933	amod	inner,segment
R8624	T12935	T12912	punct	;,GC
R8625	T12936	T12912	appos	ONL,GC
R8626	T12937	T12936	punct	", ",ONL
R8627	T12938	T12939	amod	outer,layer
R8628	T12939	T12936	appos	layer,ONL
R8629	T12940	T12939	amod	nuclear,layer
R863	T1585	T1584	punct	-,selective
R8630	T12941	T12912	punct	;,GC
R8631	T12942	T12912	appos	OPL,GC
R8632	T12943	T12942	punct	", ",OPL
R8633	T12944	T12945	amod	outer,layer
R8634	T12945	T12942	appos	layer,OPL
R8635	T12946	T12945	amod	plexiform,layer
R8636	T12947	T12945	punct	(,layer
R8637	T12948	T12945	amod	synaptic,layer
R8638	T12949	T12945	punct	),layer
R8639	T12950	T12912	punct	;,GC
R864	T1586	T1582	prep	of,effects
R8640	T12951	T12912	appos	INL,GC
R8641	T12952	T12951	punct	", ",INL
R8642	T12953	T12954	amod	inner,layer
R8643	T12954	T12951	appos	layer,INL
R8644	T12955	T12954	amod	nuclear,layer
R8645	T12956	T12912	punct	;,GC
R8646	T12957	T12912	appos	IPL,GC
R8647	T12958	T12957	punct	", ",IPL
R8648	T12959	T12960	amod	inner,layer
R8649	T12960	T12957	appos	layer,IPL
R865	T1587	T1586	pobj	RanBP2,of
R8650	T12961	T12960	amod	plexiform,layer
R8651	T12962	T12960	punct	(,layer
R8652	T12963	T12960	amod	synaptic,layer
R8653	T12964	T12960	punct	),layer
R8654	T12965	T12912	punct	;,GC
R8655	T12966	T12912	appos	GC,GC
R8656	T12967	T12966	punct	", ",GC
R8657	T12968	T12969	compound	ganglion,cell
R8658	T12969	T12970	compound	cell,layer
R8659	T12970	T12966	appos	layer,GC
R866	T1588	T1580	aux	may,underlie
R8660	T12971	T12912	punct	.,GC
R8661	T13139	T13140	nsubj	Haploinsufficiency,Causes
R8662	T13141	T13139	prep	of,Haploinsufficiency
R8663	T13142	T13141	pobj	RanBP2,of
R8664	T13143	T13144	det	a,Decrease
R8665	T13144	T13140	dobj	Decrease,Causes
R8666	T13145	T13144	prep	in,Decrease
R8667	T13146	T13147	compound	HKI,Protein
R8668	T13147	T13145	pobj	Protein,in
R8669	T13148	T13147	cc	and,Protein
R867	T1589	T1580	advmod	also,underlie
R8670	T13149	T13150	compound	ATP,Levels
R8671	T13150	T13147	conj	Levels,Protein
R8672	T13152	T13153	punct	(,A
R8673	T13153	T13154	meta	A,analysis
R8674	T13155	T13153	punct	),A
R8675	T13156	T13154	amod	Quantitative,analysis
R8676	T13157	T13154	prep	of,analysis
R8677	T13158	T13157	pobj	NPCs,of
R8678	T13159	T13154	prep	in,analysis
R8679	T13160	T13161	amod	dissociated,neurons
R868	T1590	T1591	det	the,pathogenesis
R8680	T13161	T13159	pobj	neurons,in
R8681	T13162	T13161	amod	hippocampal,neurons
R8682	T13163	T13161	prep	of,neurons
R8683	T13164	T13165	amod	wild,type
R8684	T13165	T13167	nmod	type,mice
R8685	T13166	T13165	punct	-,type
R8686	T13167	T13163	pobj	mice,of
R8687	T13168	T13169	punct	(,+
R8688	T13169	T13165	punct	+,type
R8689	T13170	T13169	punct	+,+
R869	T1591	T1580	dobj	pathogenesis,underlie
R8690	T13171	T13169	punct	/,+
R8691	T13172	T13169	punct	),+
R8692	T13173	T13165	cc	and,type
R8693	T13174	T13165	conj	heterozygote,type
R8694	T13175	T13176	punct	(,−
R8695	T13176	T13174	punct	−,heterozygote
R8696	T13177	T13176	punct	+,−
R8697	T13178	T13176	punct	/,−
R8698	T13179	T13176	punct	),−
R8699	T13180	T13154	prep	upon,analysis
R87	T326	T325	pobj	RanBP2,of
R870	T1592	T1591	prep	of,pathogenesis
R8700	T13181	T13180	pobj	immunostaining,upon
R8701	T13182	T13181	prep	with,immunostaining
R8702	T13183	T13182	pobj	mAb414,with
R8703	T13184	T13154	punct	.,analysis
R8704	T13186	T13187	det	No,difference
R8705	T13187	T13188	nsubjpass	difference,found
R8706	T13189	T13187	prep	in,difference
R8707	T13190	T13191	det	the,density
R8708	T13191	T13189	pobj	density,in
R8709	T13192	T13191	prep	of,density
R871	T1593	T1594	amod	certain,neuropathies
R8710	T13193	T13192	pobj	NPCs,of
R8711	T13194	T13191	punct	(,density
R8712	T13195	T13196	quantmod	3,4
R8713	T13196	T13198	nummod	4,NPC
R8714	T13197	T13196	punct	–,4
R8715	T13198	T13191	appos	NPC,density
R8716	T13199	T13200	punct	/,μm2
R8717	T13200	T13198	prep	μm2,NPC
R8718	T13201	T13191	punct	),density
R8719	T13202	T13191	prep	at,density
R872	T1594	T1592	pobj	neuropathies,of
R8720	T13203	T13204	det	the,envelope
R8721	T13204	T13202	pobj	envelope,at
R8722	T13205	T13204	amod	nuclear,envelope
R8723	T13206	T13188	auxpass	was,found
R8724	T13207	T13188	prep	between,found
R8725	T13208	T13209	nmod	RanBP2,mice
R8726	T13209	T13207	pobj	mice,between
R8727	T13210	T13208	punct	+,RanBP2
R8728	T13211	T13208	punct	/,RanBP2
R8729	T13212	T13208	punct	+,RanBP2
R873	T1595	T1578	punct	.,supports
R8730	T13213	T13208	cc	and,RanBP2
R8731	T13214	T13208	conj	RanBP2,RanBP2
R8732	T13215	T13214	punct	+,RanBP2
R8733	T13216	T13214	punct	/,RanBP2
R8734	T13217	T13214	punct	−,RanBP2
R8735	T13218	T13188	punct	.,found
R8736	T13220	T13221	punct	(,B
R8737	T13221	T13222	meta	B,Immunoblots
R8738	T13223	T13221	punct	),B
R8739	T13224	T13222	prep	with,Immunoblots
R874	T1597	T1598	nsubjpass	Parkin,expressed
R8740	T13225	T13226	nmod	anti-RanBP2,Nup62
R8741	T13226	T13230	nmod	Nup62,antibodies
R8742	T13227	T13226	punct	/,Nup62
R8743	T13228	T13226	nmod	Nup153,Nup62
R8744	T13229	T13226	punct	/,Nup62
R8745	T13230	T13224	pobj	antibodies,with
R8746	T13231	T13226	punct	(,Nup62
R8747	T13232	T13226	appos	mAb414,Nup62
R8748	T13233	T13226	punct	),Nup62
R8749	T13234	T13226	punct	", ",Nup62
R875	T1599	T1598	auxpass	is,expressed
R8750	T13235	T13236	punct	−,HKI
R8751	T13236	T13226	conj	HKI,Nup62
R8752	T13237	T13236	punct	", ",HKI
R8753	T13238	T13239	punct	−,mHsp70
R8754	T13239	T13236	conj	mHsp70,HKI
R8755	T13240	T13239	punct	", ",mHsp70
R8756	T13241	T13239	cc	and,mHsp70
R8757	T13242	T13243	punct	−,Cox11
R8758	T13243	T13239	conj	Cox11,mHsp70
R8759	T13244	T13230	prep	of,antibodies
R876	T1600	T1598	advmod	ubiquitously,expressed
R8760	T13245	T13246	amod	retinal,homogenates
R8761	T13246	T13244	pobj	homogenates,of
R8762	T13247	T13248	punct	(,panel
R8763	T13248	T13245	parataxis	panel,retinal
R8764	T13249	T13248	amod	top,panel
R8765	T13250	T13248	punct	),panel
R8766	T13251	T13245	cc	and,retinal
R8767	T13252	T13245	conj	hippocampal,retinal
R8768	T13253	T13246	prep	of,homogenates
R8769	T13254	T13255	punct	+,+
R877	T1601	T1598	cc	and,expressed
R8770	T13255	T13257	punct	+,mice
R8771	T13256	T13255	punct	/,+
R8772	T13257	T13253	pobj	mice,of
R8773	T13258	T13255	cc	and,+
R8774	T13259	T13260	punct	+,−
R8775	T13260	T13255	conj	−,+
R8776	T13261	T13260	punct	/,−
R8777	T13262	T13222	punct	.,Immunoblots
R8778	T13264	T13265	prep	In,exhibit
R8779	T13266	T13264	pobj	comparison,In
R878	T1602	T1598	conj	interacts,expressed
R8780	T13267	T13266	prep	to,comparison
R8781	T13268	T13267	pobj	RanBP2,to
R8782	T13269	T13268	punct	+,RanBP2
R8783	T13270	T13268	punct	/,RanBP2
R8784	T13271	T13268	punct	+,RanBP2
R8785	T13272	T13265	punct	", ",exhibit
R8786	T13273	T13274	nmod	RanBP2,mice
R8787	T13274	T13265	nsubj	mice,exhibit
R8788	T13275	T13273	punct	+,RanBP2
R8789	T13276	T13273	punct	/,RanBP2
R879	T1603	T1602	prep	with,interacts
R8790	T13277	T13273	punct	−,RanBP2
R8791	T13278	T13279	det	a,reduction
R8792	T13279	T13265	dobj	reduction,exhibit
R8793	T13280	T13279	prep	in,reduction
R8794	T13281	T13282	det	the,levels
R8795	T13282	T13280	pobj	levels,in
R8796	T13283	T13282	compound	expression,levels
R8797	T13284	T13282	prep	of,levels
R8798	T13285	T13284	pobj	RanBP2,of
R8799	T13286	T13285	cc	and,RanBP2
R88	T327	T323	cc	and,partners
R880	T1604	T1605	det	the,domain
R8800	T13287	T13285	conj	HKI,RanBP2
R8801	T13288	T13284	cc	but,of
R8802	T13289	T13288	neg	not,but
R8803	T13290	T13284	conj	of,of
R8804	T13291	T13292	amod	other,proteins
R8805	T13292	T13290	pobj	proteins,of
R8806	T13293	T13265	punct	.,exhibit
R8807	T13295	T13296	punct	(,C
R8808	T13296	T13297	meta	C,analysis
R8809	T13298	T13296	punct	),C
R881	T1605	T1603	pobj	domain,with
R8810	T13299	T13297	amod	Quantitative,analysis
R8811	T13300	T13297	prep	of,analysis
R8812	T13301	T13302	amod	relative,levels
R8813	T13302	T13300	pobj	levels,of
R8814	T13303	T13304	compound	protein,expression
R8815	T13304	T13302	compound	expression,levels
R8816	T13305	T13302	prep	of,levels
R8817	T13306	T13305	pobj	RanBP2,of
R8818	T13307	T13306	punct	", ",RanBP2
R8819	T13308	T13306	conj	Cox11,RanBP2
R882	T1606	T1607	npadvmod	Ubc9,interacting
R8820	T13309	T13308	punct	", ",Cox11
R8821	T13310	T13308	conj	HKI,Cox11
R8822	T13311	T13310	punct	", ",HKI
R8823	T13312	T13310	cc	and,HKI
R8824	T13313	T13310	conj	mHsp70,HKI
R8825	T13314	T13297	prep	in,analysis
R8826	T13315	T13316	det	the,hippocampus
R8827	T13316	T13314	pobj	hippocampus,in
R8828	T13317	T13316	prep	of,hippocampus
R8829	T13318	T13319	nmod	RanBP2,mice
R883	T1607	T1605	amod	interacting,domain
R8830	T13319	T13317	pobj	mice,of
R8831	T13320	T13318	punct	+,RanBP2
R8832	T13321	T13318	punct	/,RanBP2
R8833	T13322	T13318	punct	+,RanBP2
R8834	T13323	T13318	cc	and,RanBP2
R8835	T13324	T13318	conj	RanBP2,RanBP2
R8836	T13325	T13324	punct	+,RanBP2
R8837	T13326	T13324	punct	/,RanBP2
R8838	T13327	T13324	punct	−,RanBP2
R8839	T13328	T13297	punct	.,analysis
R884	T1608	T1607	punct	-,interacting
R8840	T13330	T13331	expl	There,is
R8841	T13332	T13333	punct	~,2
R8842	T13333	T13334	nummod	2,reduction
R8843	T13334	T13331	attr	reduction,is
R8844	T13335	T13333	punct	-,2
R8845	T13336	T13333	cc	and,2
R8846	T13337	T13333	conj	4-fold,2
R8847	T13338	T13334	prep	of,reduction
R8848	T13339	T13338	pobj	RanBP2,of
R8849	T13340	T13339	cc	and,RanBP2
R885	T1609	T1605	prep	of,domain
R8850	T13341	T13339	conj	HKI,RanBP2
R8851	T13342	T13331	prep	in,is
R8852	T13343	T13344	compound	heterozygote,mice
R8853	T13344	T13342	pobj	mice,in
R8854	T13345	T13331	punct	.,is
R8855	T13347	T13348	punct	(,D
R8856	T13348	T13349	meta	D,reduced
R8857	T13350	T13348	punct	),D
R8858	T13351	T13352	det	The,level
R8859	T13352	T13349	nsubjpass	level,reduced
R886	T1610	T1609	pobj	RanBP2,of
R8860	T13353	T13352	prep	of,level
R8861	T13354	T13353	pobj	HKI,of
R8862	T13355	T13349	auxpass	is,reduced
R8863	T13356	T13349	prep	in,reduced
R8864	T13357	T13358	det	the,brain
R8865	T13358	T13356	pobj	brain,in
R8866	T13359	T13356	cc	but,in
R8867	T13360	T13359	neg	not,but
R8868	T13361	T13356	conj	in,in
R8869	T13362	T13363	amod	other,tissues
R887	T1611	T1612	punct	[,20
R8870	T13363	T13361	pobj	tissues,in
R8871	T13364	T13363	amod	non-neuronal,tissues
R8872	T13365	T13363	acl	tested,tissues
R8873	T13366	T13363	punct	(,tissues
R8874	T13367	T13363	appos	muscle,tissues
R8875	T13368	T13367	punct	", ",muscle
R8876	T13369	T13367	conj	spleen,muscle
R8877	T13370	T13369	punct	", ",spleen
R8878	T13371	T13369	cc	and,spleen
R8879	T13372	T13369	conj	liver,spleen
R888	T1612	T1602	parataxis	20,interacts
R8880	T13373	T13349	punct	),reduced
R8881	T13374	T13349	punct	.,reduced
R8882	T13376	T13377	punct	(,E
R8883	T13377	T13378	meta	E,reduced
R8884	T13379	T13377	punct	),E
R8885	T13380	T13381	det	The,level
R8886	T13381	T13378	nsubjpass	level,reduced
R8887	T13382	T13381	amod	total,level
R8888	T13383	T13381	compound	ATP,level
R8889	T13384	T13378	auxpass	is,reduced
R889	T1613	T1612	punct	],20
R8890	T13385	T13378	prep	in,reduced
R8891	T13386	T13387	det	the,tissues
R8892	T13387	T13385	pobj	tissues,in
R8893	T13388	T13387	compound	CNS,tissues
R8894	T13389	T13387	punct	(,tissues
R8895	T13390	T13387	appos	brain,tissues
R8896	T13391	T13390	cc	and,brain
R8897	T13392	T13390	conj	retina,brain
R8898	T13393	T13385	punct	),in
R8899	T13394	T13385	cc	but,in
R89	T328	T329	det	a,role
R890	T1614	T1598	punct	.,expressed
R8900	T13395	T13394	neg	not,but
R8901	T13396	T13385	conj	in,in
R8902	T13397	T13398	amod	non-neuronal,tissues
R8903	T13398	T13396	pobj	tissues,in
R8904	T13399	T13398	acl	tested,tissues
R8905	T13400	T13398	punct	(,tissues
R8906	T13401	T13402	advmod	e.g.,spleen
R8907	T13402	T13398	appos	spleen,tissues
R8908	T13403	T13402	punct	", ",spleen
R8909	T13404	T13378	punct	),reduced
R891	T1616	T1617	nsubj	This,promotes
R8910	T13405	T13378	punct	.,reduced
R8911	T13498	T13499	nmod	RanBP2,Mice
R8912	T13499	T13503	nsubj	Mice,Exhibit
R8913	T13500	T13498	punct	+,RanBP2
R8914	T13501	T13498	punct	/,RanBP2
R8915	T13502	T13498	punct	−,RanBP2
R8916	T13504	T13499	prep	on,Mice
R8917	T13505	T13506	amod	High,Fat
R8918	T13506	T13508	compound	Fat,Diet
R8919	T13507	T13506	punct	-,Fat
R892	T1618	T1619	det	the,ubiquitination
R8920	T13508	T13504	pobj	Diet,on
R8921	T13509	T13503	dobj	Deficits,Exhibit
R8922	T13510	T13509	prep	in,Deficits
R8923	T13511	T13510	pobj	Growth,in
R8924	T13513	T13514	punct	(,A
R8925	T13514	T13515	meta	A,show
R8926	T13516	T13514	punct	),A
R8927	T13517	T13515	prep	In,show
R8928	T13518	T13517	pobj	comparison,In
R8929	T13519	T13518	prep	to,comparison
R893	T1619	T1617	dobj	ubiquitination,promotes
R8930	T13520	T13521	amod	wild,type
R8931	T13521	T13523	compound	type,mice
R8932	T13522	T13521	punct	-,type
R8933	T13523	T13519	pobj	mice,to
R8934	T13524	T13515	punct	", ",show
R8935	T13525	T13526	nmod	RanBP2,mice
R8936	T13526	T13515	nsubj	mice,show
R8937	T13527	T13525	punct	+,RanBP2
R8938	T13528	T13525	punct	/,RanBP2
R8939	T13529	T13525	punct	−,RanBP2
R894	T1620	T1619	cc	and,ubiquitination
R8940	T13530	T13531	amod	slower,rates
R8941	T13531	T13515	dobj	rates,show
R8942	T13532	T13531	compound	growth,rates
R8943	T13533	T13515	advcl	beginning,show
R8944	T13534	T13533	prep	at,beginning
R8945	T13535	T13536	nummod	4,mo
R8946	T13536	T13534	pobj	mo,at
R8947	T13537	T13536	prep	of,mo
R8948	T13538	T13537	pobj	age,of
R8949	T13539	T13540	punct	(,arrow
R895	T1621	T1619	conj	degradation,ubiquitination
R8950	T13540	T13515	parataxis	arrow,show
R8951	T13541	T13540	punct	),arrow
R8952	T13542	T13515	punct	", ",show
R8953	T13543	T13515	cc	and,show
R8954	T13544	T13545	det	the,difference
R8955	T13545	T13546	nsubjpass	difference,maintained
R8956	T13546	T13515	conj	maintained,show
R8957	T13547	T13545	prep	in,difference
R8958	T13548	T13549	compound	body,weight
R8959	T13549	T13547	pobj	weight,in
R896	T1622	T1619	prep	of,ubiquitination
R8960	T13550	T13545	prep	between,difference
R8961	T13551	T13550	pobj	these,between
R8962	T13552	T13546	auxpass	is,maintained
R8963	T13553	T13546	advmod	afterward,maintained
R8964	T13554	T13546	punct	.,maintained
R8965	T13557	T13558	mark	that,lack
R8966	T13558	T13556	ccomp	lack,Note
R8967	T13559	T13560	nmod	RanBP2,mice
R8968	T13560	T13558	nsubj	mice,lack
R8969	T13561	T13559	punct	+,RanBP2
R897	T1623	T1622	pobj	RanBP2,of
R8970	T13562	T13559	punct	/,RanBP2
R8971	T13563	T13559	punct	−,RanBP2
R8972	T13564	T13565	det	the,spur
R8973	T13565	T13558	dobj	spur,lack
R8974	T13566	T13565	compound	growth,spur
R8975	T13567	T13565	acl	observed,spur
R8976	T13568	T13567	prep	in,observed
R8977	T13569	T13570	amod	wild,type
R8978	T13570	T13572	compound	type,mice
R8979	T13571	T13570	punct	-,type
R898	T1624	T1625	punct	[,20
R8980	T13572	T13568	pobj	mice,in
R8981	T13573	T13572	prep	between,mice
R8982	T13574	T13575	nummod	3,mo
R8983	T13575	T13573	pobj	mo,between
R8984	T13576	T13574	cc	and,3
R8985	T13577	T13574	conj	4,3
R8986	T13578	T13575	prep	of,mo
R8987	T13579	T13578	pobj	age,of
R8988	T13580	T13556	punct	.,Note
R8989	T13582	T13583	punct	(,B
R899	T1625	T1619	parataxis	20,ubiquitination
R8990	T13583	T13584	meta	B,masked
R8991	T13585	T13583	punct	),B
R8992	T13586	T13584	prep	In,masked
R8993	T13587	T13586	pobj	comparison,In
R8994	T13588	T13587	prep	to,comparison
R8995	T13589	T13590	amod	inbred,mice
R8996	T13590	T13588	pobj	mice,to
R8997	T13591	T13590	nmod	RanBP2,mice
R8998	T13592	T13591	punct	+,RanBP2
R8999	T13593	T13591	punct	/,RanBP2
R9	T238	T237	nummod	I,Hexokinase
R90	T329	T323	conj	role,partners
R900	T1626	T1625	punct	],20
R9000	T13594	T13591	punct	−,RanBP2
R9001	T13595	T13590	punct	(,mice
R9002	T13596	T13597	nmod	129Ola,background
R9003	T13597	T13590	appos	background,mice
R9004	T13598	T13597	amod	genetic,background
R9005	T13599	T13584	punct	),masked
R9006	T13600	T13584	punct	", ",masked
R9007	T13601	T13602	det	the,difference
R9008	T13602	T13584	nsubjpass	difference,masked
R9009	T13603	T13602	prep	in,difference
R901	T1627	T1617	punct	", ",promotes
R9010	T13604	T13605	compound	body,weight
R9011	T13605	T13603	pobj	weight,in
R9012	T13606	T13602	prep	between,difference
R9013	T13607	T13608	nmod	RanBP2,mice
R9014	T13608	T13606	pobj	mice,between
R9015	T13609	T13607	punct	+,RanBP2
R9016	T13610	T13607	punct	/,RanBP2
R9017	T13611	T13607	punct	+,RanBP2
R9018	T13612	T13607	cc	and,RanBP2
R9019	T13613	T13607	conj	RanBP2,RanBP2
R902	T1628	T1629	advmod	possibly,via
R9020	T13614	T13613	punct	+,RanBP2
R9021	T13615	T13613	punct	/,RanBP2
R9022	T13616	T13613	punct	−,RanBP2
R9023	T13617	T13584	auxpass	is,masked
R9024	T13618	T13584	prep	upon,masked
R9025	T13619	T13618	pcomp	placing,upon
R9026	T13620	T13619	dobj	these,placing
R9027	T13621	T13619	prep	on,placing
R9028	T13622	T13623	det	a,background
R9029	T13623	T13621	pobj	background,on
R903	T1629	T1617	prep	via,promotes
R9030	T13624	T13623	amod	mixed,background
R9031	T13625	T13626	nmod	129Ola,C57Bl6
R9032	T13626	T13623	nmod	C57Bl6,background
R9033	T13627	T13626	punct	/,C57Bl6
R9034	T13628	T13623	amod	genetic,background
R9035	T13629	T13584	punct	.,masked
R9036	T13631	T13632	punct	(,C
R9037	T13632	T13633	meta	C,exhibit
R9038	T13634	T13632	punct	),C
R9039	T13635	T13636	nmod	RanBP2,mice
R904	T1630	T1631	det	the,interaction
R9040	T13636	T13633	nsubj	mice,exhibit
R9041	T13637	T13635	punct	+,RanBP2
R9042	T13638	T13635	punct	/,RanBP2
R9043	T13639	T13635	punct	+,RanBP2
R9044	T13640	T13635	cc	and,RanBP2
R9045	T13641	T13635	conj	RanBP2,RanBP2
R9046	T13642	T13641	punct	+,RanBP2
R9047	T13643	T13641	punct	/,RanBP2
R9048	T13644	T13641	punct	−,RanBP2
R9049	T13645	T13636	amod	inbred,mice
R905	T1631	T1629	pobj	interaction,via
R9050	T13646	T13647	amod	similar,rates
R9051	T13647	T13633	dobj	rates,exhibit
R9052	T13648	T13647	prep	of,rates
R9053	T13649	T13650	compound	food,consumption
R9054	T13650	T13648	pobj	consumption,of
R9055	T13651	T13633	punct	.,exhibit
R9056	T13653	T13654	nsubjpass	Mice,placed
R9057	T13655	T13653	prep	in,Mice
R9058	T13656	T13655	punct	(,in
R9059	T13657	T13655	pobj	A,in
R906	T1632	T1631	prep	of,interaction
R9060	T13658	T13657	punct	),A
R9061	T13659	T13657	punct	", ",A
R9062	T13660	T13661	punct	(,B
R9063	T13661	T13657	conj	B,A
R9064	T13662	T13661	punct	),B
R9065	T13663	T13661	punct	", ",B
R9066	T13664	T13661	cc	and,B
R9067	T13665	T13666	punct	(,C
R9068	T13666	T13661	conj	C,B
R9069	T13667	T13654	punct	),placed
R907	T1633	T1634	det	the,domain
R9070	T13668	T13654	auxpass	were,placed
R9071	T13669	T13654	prep	on,placed
R9072	T13670	T13671	det	a,diet
R9073	T13671	T13669	pobj	diet,on
R9074	T13672	T13673	amod	high,fat
R9075	T13673	T13671	compound	fat,diet
R9076	T13674	T13673	punct	-,fat
R9077	T13675	T13654	prep	since,placed
R9078	T13676	T13675	pobj	birth,since
R9079	T13677	T13678	punct	(,5
R908	T1634	T1632	pobj	domain,of
R9080	T13678	T13654	parataxis	5,placed
R9081	T13679	T13678	nsubj	n,5
R9082	T13680	T13678	punct	=,5
R9083	T13681	T13678	punct	),5
R9084	T13682	T13654	punct	.,placed
R9085	T13772	T13773	amod	Metabolic,Phenotypes
R9086	T13774	T13773	prep	of,Phenotypes
R9087	T13775	T13776	nmod	RanBP2,Mice
R9088	T13776	T13774	pobj	Mice,of
R9089	T13777	T13775	punct	+,RanBP2
R909	T1635	T1634	compound	CLD,domain
R9090	T13778	T13775	punct	/,RanBP2
R9091	T13779	T13775	punct	−,RanBP2
R9092	T13780	T13776	amod	Inbred,Mice
R9093	T13781	T13776	prep	on,Mice
R9094	T13782	T13783	amod	High,Fat
R9095	T13783	T13785	compound	Fat,Diet
R9096	T13784	T13783	punct	-,Fat
R9097	T13785	T13781	pobj	Diet,on
R9098	T13787	T13788	punct	(,A
R9099	T13788	T13789	meta	A,have
R91	T330	T329	amod	novel,role
R910	T1636	T1634	prep	of,domain
R9100	T13790	T13788	punct	),A
R9101	T13791	T13792	nummod	3,mo
R9102	T13792	T13794	npadvmod	mo,old
R9103	T13793	T13792	punct	-,mo
R9104	T13794	T13796	amod	old,mice
R9105	T13795	T13794	punct	-,old
R9106	T13796	T13789	nsubj	mice,have
R9107	T13797	T13796	amod	inbred,mice
R9108	T13798	T13796	nmod	RanBP2,mice
R9109	T13799	T13798	punct	+,RanBP2
R911	T1637	T1636	pobj	RanBP2,of
R9110	T13800	T13798	punct	/,RanBP2
R9111	T13801	T13798	punct	−,RanBP2
R9112	T13802	T13803	punct	(,5
R9113	T13803	T13796	parataxis	5,mice
R9114	T13804	T13803	nsubj	n,5
R9115	T13805	T13803	punct	=,5
R9116	T13806	T13803	punct	),5
R9117	T13807	T13808	amod	normal,rates
R9118	T13808	T13789	dobj	rates,have
R9119	T13809	T13810	compound	glucose,clearance
R912	T1638	T1631	prep	with,interaction
R9120	T13810	T13808	compound	clearance,rates
R9121	T13811	T13789	prep	upon,have
R9122	T13812	T13813	compound	glucose,challenge
R9123	T13813	T13811	pobj	challenge,upon
R9124	T13814	T13813	cc	and,challenge
R9125	T13815	T13816	amod	overnight,fasting
R9126	T13816	T13813	conj	fasting,challenge
R9127	T13817	T13789	punct	.,have
R9128	T13819	T13820	punct	(,B
R9129	T13820	T13821	meta	B,have
R913	T1639	T1640	det	the,subunits
R9130	T13822	T13820	punct	),B
R9131	T13823	T13821	prep	In,have
R9132	T13824	T13823	pobj	contrast,In
R9133	T13825	T13821	punct	", ",have
R9134	T13826	T13827	nummod	6,mo
R9135	T13827	T13829	npadvmod	mo,old
R9136	T13828	T13827	punct	-,mo
R9137	T13829	T13831	amod	old,mice
R9138	T13830	T13829	punct	-,old
R9139	T13831	T13821	nsubj	mice,have
R914	T1640	T1638	pobj	subunits,with
R9140	T13832	T13831	amod	inbred,mice
R9141	T13833	T13831	nmod	RanBP2,mice
R9142	T13834	T13833	punct	+,RanBP2
R9143	T13835	T13833	punct	/,RanBP2
R9144	T13836	T13833	punct	−,RanBP2
R9145	T13837	T13838	punct	(,5
R9146	T13838	T13831	parataxis	5,mice
R9147	T13839	T13838	nsubj	n,5
R9148	T13840	T13838	punct	=,5
R9149	T13841	T13838	punct	),5
R915	T1641	T1642	compound	19S,cap
R9150	T13842	T13843	advmod	significantly,decreased
R9151	T13843	T13844	amod	decreased,rates
R9152	T13844	T13821	dobj	rates,have
R9153	T13845	T13846	compound	glucose,clearance
R9154	T13846	T13844	compound	clearance,rates
R9155	T13847	T13821	prep	upon,have
R9156	T13848	T13849	compound	glucose,challenge
R9157	T13849	T13847	pobj	challenge,upon
R9158	T13850	T13849	cc	and,challenge
R9159	T13851	T13852	amod	overnight,fasting
R916	T1642	T1640	compound	cap,subunits
R9160	T13852	T13849	conj	fasting,challenge
R9161	T13853	T13821	punct	.,have
R9162	T13855	T13856	punct	(,C
R9163	T13856	T13857	meta	C,have
R9164	T13858	T13856	punct	),C
R9165	T13859	T13860	amod	Fasted,mice
R9166	T13860	T13857	nsubj	mice,have
R9167	T13861	T13862	quantmod	6,8
R9168	T13862	T13865	nummod	8,mo
R9169	T13863	T13862	punct	-,8
R917	T1643	T1640	prep	of,subunits
R9170	T13864	T13862	quantmod	to,8
R9171	T13865	T13867	npadvmod	mo,old
R9172	T13866	T13865	punct	-,mo
R9173	T13867	T13860	amod	old,mice
R9174	T13868	T13867	punct	-,old
R9175	T13869	T13860	nmod	RanBP2,mice
R9176	T13870	T13869	punct	+,RanBP2
R9177	T13871	T13869	punct	/,RanBP2
R9178	T13872	T13869	punct	+,RanBP2
R9179	T13873	T13869	cc	and,RanBP2
R918	T1644	T1645	det	the,proteasome
R9180	T13874	T13869	conj	RanBP2,RanBP2
R9181	T13875	T13874	punct	+,RanBP2
R9182	T13876	T13874	punct	/,RanBP2
R9183	T13877	T13874	punct	−,RanBP2
R9184	T13878	T13879	det	no,difference
R9185	T13879	T13857	dobj	difference,have
R9186	T13880	T13879	prep	in,difference
R9187	T13881	T13882	npadvmod	insulin,mediated
R9188	T13882	T13884	amod	mediated,uptake
R9189	T13883	T13882	punct	-,mediated
R919	T1645	T1643	pobj	proteasome,of
R9190	T13884	T13880	pobj	uptake,in
R9191	T13885	T13884	compound	glucose,uptake
R9192	T13886	T13887	mark	as,assayed
R9193	T13887	T13857	advcl	assayed,have
R9194	T13888	T13887	prep	by,assayed
R9195	T13889	T13890	compound	insulin,tolerance
R9196	T13890	T13891	compound	tolerance,test
R9197	T13891	T13888	pobj	test,by
R9198	T13892	T13893	punct	(,5
R9199	T13893	T13857	parataxis	5,have
R92	T331	T329	amod	physiological,role
R920	T1646	T1647	punct	[,14
R9200	T13894	T13893	nsubj	n,5
R9201	T13895	T13893	punct	=,5
R9202	T13896	T13893	punct	),5
R9203	T13897	T13857	punct	.,have
R9204	T13899	T13900	punct	(,D
R9205	T13900	T13901	meta	D,shows
R9206	T13902	T13900	punct	),D
R9207	T13903	T13904	compound	Pyruvate,tolerance
R9208	T13904	T13905	compound	tolerance,test
R9209	T13905	T13901	nsubj	test,shows
R921	T1647	T1631	parataxis	14,interaction
R9210	T13906	T13907	amod	normal,rise
R9211	T13907	T13901	dobj	rise,shows
R9212	T13908	T13907	prep	in,rise
R9213	T13909	T13908	pobj	glucose,in
R9214	T13910	T13907	cc	but,rise
R9215	T13911	T13912	amod	decreased,clearance
R9216	T13912	T13907	conj	clearance,rise
R9217	T13913	T13912	compound	glucose,clearance
R9218	T13914	T13907	prep	between,rise
R9219	T13915	T13916	amod	inbred,mice
R922	T1648	T1647	punct	],14
R9220	T13916	T13914	pobj	mice,between
R9221	T13917	T13916	nmod	RanBP2,mice
R9222	T13918	T13917	punct	+,RanBP2
R9223	T13919	T13917	punct	/,RanBP2
R9224	T13920	T13917	punct	+,RanBP2
R9225	T13921	T13917	cc	and,RanBP2
R9226	T13922	T13917	conj	RanBP2,RanBP2
R9227	T13923	T13922	punct	+,RanBP2
R9228	T13924	T13922	punct	/,RanBP2
R9229	T13925	T13922	punct	−,RanBP2
R923	T1649	T1617	punct	.,promotes
R9230	T13926	T13927	punct	(,5
R9231	T13927	T13901	parataxis	5,shows
R9232	T13928	T13927	nsubj	n,5
R9233	T13929	T13927	punct	=,5
R9234	T13930	T13927	punct	),5
R9235	T13931	T13901	punct	.,shows
R924	T1651	T1652	advmod	Interestingly,required
R9244	T14066	T14065	prep	from,Electroretinograms
R9245	T14067	T14068	nummod	6,Mo
R9246	T14068	T14070	npadvmod	Mo,Old
R9247	T14069	T14068	punct	-,Mo
R9248	T14070	T14072	amod	Old,Mice
R9249	T14071	T14070	punct	-,Old
R925	T1653	T1652	punct	", ",required
R9250	T14072	T14066	pobj	Mice,from
R9251	T14073	T14072	nmod	RanBP2,Mice
R9252	T14074	T14073	punct	+,RanBP2
R9253	T14075	T14073	punct	/,RanBP2
R9254	T14076	T14073	punct	−,RanBP2
R9255	T14077	T14073	cc	and,RanBP2
R9256	T14078	T14073	conj	RanBP2,RanBP2
R9257	T14079	T14078	punct	+,RanBP2
R9258	T14080	T14078	punct	/,RanBP2
R9259	T14081	T14078	punct	+,RanBP2
R926	T1654	T1655	det	the,protein
R9260	T14082	T14072	amod	Inbred,Mice
R9261	T14083	T14072	acl	Showing,Mice
R9262	T14084	T14085	nmod	Photoreceptor,Neuron
R9263	T14085	T14088	nmod	Neuron,Phenotypes
R9264	T14086	T14084	cc	and,Photoreceptor
R9265	T14087	T14084	conj	Postreceptor,Photoreceptor
R9266	T14088	T14083	dobj	Phenotypes,Showing
R9267	T14089	T14090	amod	Electrophysiological,Response
R9268	T14090	T14088	compound	Response,Phenotypes
R9269	T14092	T14093	punct	(,A
R927	T1655	T1652	nsubjpass	protein,required
R9270	T14093	T14094	meta	A,have
R9271	T14095	T14093	punct	),A
R9272	T14096	T14097	amod	Scotopic,responses
R9273	T14097	T14094	nsubj	responses,have
R9274	T14098	T14099	punct	(,adapted
R9275	T14099	T14096	parataxis	adapted,Scotopic
R9276	T14100	T14099	dep	dark,adapted
R9277	T14101	T14099	punct	-,adapted
R9278	T14102	T14099	punct	),adapted
R9279	T14103	T14097	prep	from,responses
R928	T1656	T1655	amod	small,protein
R9280	T14104	T14105	nmod	RanBP2,mice
R9281	T14105	T14103	pobj	mice,from
R9282	T14106	T14104	punct	+,RanBP2
R9283	T14107	T14104	punct	/,RanBP2
R9284	T14108	T14104	punct	−,RanBP2
R9285	T14109	T14097	prep	to,responses
R9286	T14110	T14111	amod	light,stimuli
R9287	T14111	T14109	pobj	stimuli,to
R9288	T14112	T14111	prep	of,stimuli
R9289	T14113	T14114	amod	increasing,intensity
R929	T1657	T1655	nmod	yeast,protein
R9290	T14114	T14112	pobj	intensity,of
R9291	T14115	T14094	punct	", ",have
R9292	T14116	T14094	advcl	beginning,have
R9293	T14117	T14116	prep	at,beginning
R9294	T14118	T14117	pobj	threshold,at
R9295	T14119	T14094	punct	", ",have
R9296	T14120	T14121	amod	reduced,amplitudes
R9297	T14121	T14094	dobj	amplitudes,have
R9298	T14122	T14094	prep	compared,have
R9299	T14123	T14122	prep	to,compared
R93	T332	T329	prep	of,role
R930	T1658	T1659	npadvmod	Ran,binding
R9300	T14124	T14123	pobj	those,to
R9301	T14125	T14124	acl	observed,those
R9302	T14126	T14125	prep	in,observed
R9303	T14127	T14128	nmod	RanBP2,mice
R9304	T14128	T14126	pobj	mice,in
R9305	T14129	T14127	punct	+,RanBP2
R9306	T14130	T14127	punct	/,RanBP2
R9307	T14131	T14127	punct	+,RanBP2
R9308	T14132	T14094	punct	.,have
R9309	T14134	T14135	det	The,intensities
R931	T1659	T1655	amod	binding,protein
R9310	T14135	T14138	nsubj	intensities,represent
R9311	T14136	T14135	nummod	three,intensities
R9312	T14137	T14135	amod	lower,intensities
R9313	T14139	T14138	dobj	responses,represent
R9314	T14140	T14139	acl	generated,responses
R9315	T14141	T14140	prep	in,generated
R9316	T14142	T14143	det	the,pathway
R9317	T14143	T14141	pobj	pathway,in
R9318	T14144	T14145	nmod	rod,photoreceptor
R9319	T14145	T14143	nmod	photoreceptor,pathway
R932	T1660	T1659	punct	-,binding
R9320	T14146	T14143	amod	neuronal,pathway
R9321	T14147	T14138	punct	.,represent
R9322	T14149	T14150	det	The,intensities
R9323	T14150	T14152	nsubjpass	intensities,comprised
R9324	T14151	T14150	amod	upper,intensities
R9325	T14153	T14152	auxpass	are,comprised
R9326	T14154	T14152	prep	of,comprised
R9327	T14155	T14154	pobj	responses,of
R9328	T14156	T14155	acl	generated,responses
R9329	T14157	T14156	prep	in,generated
R933	T1661	T1655	nummod	1,protein
R9330	T14158	T14159	preconj	both,pathways
R9331	T14159	T14157	pobj	pathways,in
R9332	T14160	T14159	det	the,pathways
R9333	T14161	T14159	nmod	rod,pathways
R9334	T14162	T14161	cc	and,rod
R9335	T14163	T14161	conj	cone,rod
R9336	T14164	T14152	punct	.,comprised
R9337	T14166	T14167	punct	(,B
R9338	T14167	T14168	meta	B,exhibited
R9339	T14169	T14167	punct	),B
R934	T1662	T1655	punct	(,protein
R9340	T14170	T14171	amod	Photopic,responses
R9341	T14171	T14168	nsubj	responses,exhibited
R9342	T14172	T14173	punct	(,pathway
R9343	T14173	T14170	parataxis	pathway,Photopic
R9344	T14174	T14175	npadvmod	light,adapted
R9345	T14175	T14173	amod	adapted,pathway
R9346	T14176	T14175	punct	-,adapted
R9347	T14177	T14173	punct	", ",pathway
R9348	T14178	T14179	compound	cone,photoreceptor
R9349	T14179	T14173	compound	photoreceptor,pathway
R935	T1663	T1655	appos	RanBP1,protein
R9350	T14180	T14173	punct	),pathway
R9351	T14181	T14171	prep	of,responses
R9352	T14182	T14183	nmod	RanBP2,mice
R9353	T14183	T14181	pobj	mice,of
R9354	T14184	T14182	punct	+,RanBP2
R9355	T14185	T14182	punct	/,RanBP2
R9356	T14186	T14182	punct	−,RanBP2
R9357	T14187	T14171	prep	to,responses
R9358	T14188	T14189	amod	increasing,intensities
R9359	T14189	T14187	pobj	intensities,to
R936	T1664	T1655	punct	),protein
R9360	T14190	T14191	amod	light,stimulus
R9361	T14191	T14189	compound	stimulus,intensities
R9362	T14192	T14168	advmod	also,exhibited
R9363	T14193	T14194	amod	reduced,amplitudes
R9364	T14194	T14168	dobj	amplitudes,exhibited
R9365	T14195	T14168	prep	compared,exhibited
R9366	T14196	T14195	prep	to,compared
R9367	T14197	T14196	pobj	those,to
R9368	T14198	T14197	acl	observed,those
R9369	T14199	T14198	prep	in,observed
R937	T1665	T1655	punct	", ",protein
R9370	T14200	T14201	nmod	RanBP2,mice
R9371	T14201	T14199	pobj	mice,in
R9372	T14202	T14200	punct	+,RanBP2
R9373	T14203	T14200	punct	/,RanBP2
R9374	T14204	T14200	punct	+,RanBP2
R9375	T14205	T14168	punct	.,exhibited
R9376	T14207	T14208	punct	(,C
R9377	T14208	T14209	meta	C,amplitudes
R9378	T14210	T14208	punct	),C
R9379	T14211	T14212	nmod	Average,SE
R938	T1666	T1655	appos	Yrb1p,protein
R9380	T14212	T14209	nmod	SE,amplitudes
R9381	T14213	T14212	punct	±,SE
R9382	T14214	T14215	punct	(,9
R9383	T14215	T14212	parataxis	9,SE
R9384	T14216	T14215	nsubj	n,9
R9385	T14217	T14215	punct	=,9
R9386	T14218	T14215	punct	),9
R9387	T14219	T14209	amod	scotopic,amplitudes
R9388	T14220	T14221	compound	b,wave
R9389	T14221	T14209	compound	wave,amplitudes
R939	T1667	T1655	punct	", ",protein
R9390	T14222	T14221	punct	-,wave
R9391	T14223	T14209	prep	from,amplitudes
R9392	T14224	T14225	nmod	RanBP2,mice
R9393	T14225	T14223	pobj	mice,from
R9394	T14226	T14224	punct	+,RanBP2
R9395	T14227	T14224	punct	/,RanBP2
R9396	T14228	T14224	punct	−,RanBP2
R9397	T14229	T14230	punct	(,circles
R9398	T14230	T14224	parataxis	circles,RanBP2
R9399	T14231	T14230	amod	open,circles
R94	T333	T332	pobj	RanBP2,of
R940	T1668	T1655	prep	with,protein
R9400	T14232	T14230	punct	),circles
R9401	T14233	T14224	cc	and,RanBP2
R9402	T14234	T14224	conj	RanBP2,RanBP2
R9403	T14235	T14234	punct	+,RanBP2
R9404	T14236	T14234	punct	/,RanBP2
R9405	T14237	T14234	punct	+,RanBP2
R9406	T14238	T14239	punct	(,squares
R9407	T14239	T14234	parataxis	squares,RanBP2
R9408	T14240	T14239	amod	filled,squares
R9409	T14241	T14239	punct	),squares
R941	T1669	T1670	amod	strong,homology
R9410	T14242	T14209	acl	representing,amplitudes
R9411	T14243	T14244	amod	postreceptoral,neuron
R9412	T14244	T14245	compound	neuron,function
R9413	T14245	T14242	dobj	function,representing
R9414	T14246	T14209	punct	.,amplitudes
R9415	T14248	T14249	punct	(,scale
R9416	T14250	T14249	dep	Note,scale
R9417	T14251	T14249	punct	: ,scale
R9418	T14252	T14253	compound	log,amplitude
R9419	T14253	T14249	compound	amplitude,scale
R942	T1670	T1668	pobj	homology,with
R9420	T14254	T14249	punct	.,scale
R9421	T14255	T14249	punct	),scale
R9422	T14257	T14258	punct	(,D
R9423	T14258	T14259	meta	D,amplitudes
R9424	T14260	T14258	punct	),D
R9425	T14261	T14262	amod	Average,SE
R9426	T14262	T14259	nmod	SE,amplitudes
R9427	T14263	T14262	punct	±,SE
R9428	T14264	T14265	punct	(,5
R9429	T14265	T14262	parataxis	5,SE
R943	T1671	T1670	prep	to,homology
R9430	T14266	T14265	nsubj	n,5
R9431	T14267	T14265	punct	=,5
R9432	T14268	T14265	punct	),5
R9433	T14269	T14259	amod	scotopic,amplitudes
R9434	T14270	T14271	compound	a,wave
R9435	T14271	T14259	compound	wave,amplitudes
R9436	T14272	T14271	punct	-,wave
R9437	T14273	T14259	punct	", ",amplitudes
R9438	T14274	T14259	acl	representing,amplitudes
R9439	T14275	T14276	compound	photoreceptor,function
R944	T1672	T1673	det	the,domains
R9440	T14276	T14274	dobj	function,representing
R9441	T14277	T14274	punct	", ",representing
R9442	T14278	T14274	prep	for,representing
R9443	T14279	T14280	nmod	RanBP2,mice
R9444	T14280	T14278	pobj	mice,for
R9445	T14281	T14279	punct	+,RanBP2
R9446	T14282	T14279	punct	/,RanBP2
R9447	T14283	T14279	punct	−,RanBP2
R9448	T14284	T14279	cc	and,RanBP2
R9449	T14285	T14279	conj	RanBP2,RanBP2
R945	T1673	T1671	pobj	domains,to
R9450	T14286	T14285	punct	+,RanBP2
R9451	T14287	T14285	punct	/,RanBP2
R9452	T14288	T14285	punct	+,RanBP2
R9453	T14289	T14274	prep	in,representing
R9454	T14290	T14289	pobj	response,in
R9455	T14291	T14290	prep	to,response
R9456	T14292	T14293	amod	bright,flashes
R9457	T14293	T14291	pobj	flashes,to
R9458	T14294	T14259	punct	.,amplitudes
R9459	T14296	T14297	nsubj	Amplitudes,were
R946	T1674	T1675	npadvmod	Ran,binding
R9460	T14298	T14296	prep	of,Amplitudes
R9461	T14299	T14298	pobj	responses,of
R9462	T14300	T14299	prep	from,responses
R9463	T14301	T14302	nmod	RanBP2,mice
R9464	T14302	T14300	pobj	mice,from
R9465	T14303	T14301	punct	+,RanBP2
R9466	T14304	T14301	punct	/,RanBP2
R9467	T14305	T14301	punct	−,RanBP2
R9468	T14306	T14297	acomp	lower,were
R9469	T14307	T14297	prep	over,were
R947	T1675	T1673	amod	binding,domains
R9470	T14308	T14309	det	the,range
R9471	T14309	T14307	pobj	range,over
R9472	T14310	T14309	amod	entire,range
R9473	T14311	T14309	prep	of,range
R9474	T14312	T14313	compound	stimulus,intensities
R9475	T14313	T14311	pobj	intensities,of
R9476	T14314	T14313	prep	for,intensities
R9477	T14315	T14316	preconj	both,b
R9478	T14316	T14317	nmod	b,waves
R9479	T14317	T14314	pobj	waves,for
R948	T1676	T1675	punct	-,binding
R9480	T14318	T14316	punct	-,b
R9481	T14319	T14316	cc	and,b
R9482	T14320	T14316	conj	a,b
R9483	T14321	T14320	punct	-,a
R9484	T14322	T14297	punct	.,were
R9485	T14324	T14325	nsubj	Asterisks,represent
R9486	T14326	T14327	amod	significant,differences
R9487	T14327	T14325	dobj	differences,represent
R9488	T14328	T14327	prep	between,differences
R9489	T14329	T14330	det	the,groups
R949	T1677	T1673	prep	of,domains
R9490	T14330	T14328	pobj	groups,between
R9491	T14331	T14332	punct	(,test
R9492	T14332	T14325	parataxis	test,represent
R9493	T14333	T14332	poss	Student,test
R9494	T14334	T14333	case	's,Student
R9495	T14335	T14332	compound	t,test
R9496	T14336	T14332	punct	", ",test
R9497	T14337	T14338	nsubj	p,0.05
R9498	T14338	T14332	ccomp	0.05,test
R9499	T14339	T14338	punct	<,0.05
R95	T334	T329	prep	in,role
R950	T1678	T1677	pobj	RanBP2,of
R9500	T14340	T14332	punct	),test
R9501	T14341	T14325	punct	.,represent
R9502	T14343	T14344	amod	Statistical,significance
R9503	T14344	T14345	nsubjpass	significance,found
R9504	T14346	T14345	auxpass	was,found
R9505	T14347	T14345	prep	across,found
R9506	T14348	T14349	det	all,intensities
R9507	T14349	T14347	pobj	intensities,across
R9508	T14350	T14345	prep	for,found
R9509	T14351	T14352	compound	b,wave
R951	T1679	T1652	punct	", ",required
R9510	T14352	T14354	compound	wave,amplitudes
R9511	T14353	T14352	punct	-,wave
R9512	T14354	T14350	pobj	amplitudes,for
R9513	T14355	T14356	punct	(,ANOVA
R9514	T14356	T14354	parataxis	ANOVA,amplitudes
R9515	T14357	T14358	nummod	2,way
R9516	T14358	T14356	compound	way,ANOVA
R9517	T14359	T14358	punct	-,way
R9518	T14360	T14356	punct	", ",ANOVA
R9519	T14361	T14362	nsubj	p,0.0001
R952	T1680	T1652	auxpass	is,required
R9520	T14362	T14356	ccomp	0.0001,ANOVA
R9521	T14363	T14362	punct	<,0.0001
R9522	T14364	T14356	punct	),ANOVA
R9523	T14365	T14350	punct	", ",for
R9524	T14366	T14350	cc	but,for
R9525	T14367	T14366	neg	not,but
R9526	T14368	T14350	conj	for,for
R9527	T14369	T14370	det	the,wave
R9528	T14370	T14368	pobj	wave,for
R9529	T14371	T14370	compound	a,wave
R953	T1681	T1652	advmod	also,required
R9530	T14372	T14370	punct	-,wave
R9531	T14373	T14345	punct	.,found
R9536	T14526	T14525	acl	Depicting,Model
R9537	T14527	T14528	det	a,Role
R9538	T14528	T14526	dobj	Role,Depicting
R9539	T14529	T14528	prep	of,Role
R954	T1682	T1652	prep	for,required
R9540	T14530	T14529	pobj	RanBP2,of
R9541	T14531	T14530	cc	and,RanBP2
R9542	T14532	T14530	conj	Some,RanBP2
R9543	T14533	T14532	prep	of,Some
R9544	T14534	T14535	poss	Its,Partners
R9545	T14535	T14533	pobj	Partners,of
R9546	T14536	T14528	prep	in,Role
R9547	T14537	T14538	amod	Metabolic,Function
R9548	T14538	T14536	pobj	Function,in
R9549	T14539	T14537	cc	and,Metabolic
R955	T1683	T1684	compound	cell,cycle
R9550	T14540	T14537	conj	Neuronal,Metabolic
R9551	T14542	T14543	nsubj	RanBP2,interacts
R9552	T14544	T14543	prep	with,interacts
R9553	T14545	T14544	pobj	Cox11,with
R9554	T14546	T14545	cc	and,Cox11
R9555	T14547	T14545	conj	HKI,Cox11
R9556	T14548	T14543	cc	and,interacts
R9557	T14549	T14550	det	the,triad
R9558	T14550	T14551	nsubj	triad,is
R9559	T14551	T14543	conj	is,interacts
R956	T1684	T1686	npadvmod	cycle,regulated
R9560	T14552	T14551	prep	in,is
R9561	T14553	T14552	pobj	equilibrium,in
R9562	T14554	T14551	prep	under,is
R9563	T14555	T14556	amod	normal,conditions
R9564	T14556	T14554	pobj	conditions,under
R9565	T14557	T14556	amod	physiological,conditions
R9566	T14558	T14551	punct	.,is
R9567	T14560	T14561	nsubj	RanBP2,prevents
R9568	T14562	T14563	det	the,inhibition
R9569	T14563	T14561	dobj	inhibition,prevents
R957	T1685	T1684	punct	-,cycle
R9570	T14564	T14563	prep	of,inhibition
R9571	T14565	T14564	pobj	HKI,of
R9572	T14566	T14563	prep	by,inhibition
R9573	T14567	T14566	pobj	Cox11,by
R9574	T14568	T14567	cc	and,Cox11
R9575	T14569	T14570	poss	its,degradation
R9576	T14570	T14567	conj	degradation,Cox11
R9577	T14571	T14561	punct	.,prevents
R9578	T14573	T14574	det	The,effect
R9579	T14574	T14576	nsubj	effect,is
R958	T1686	T1687	amod	regulated,degradation
R9580	T14575	T14574	amod	ultimate,effect
R9581	T14577	T14574	prep	of,effect
R9582	T14578	T14577	pobj	RanBP2,of
R9583	T14579	T14574	prep	on,effect
R9584	T14580	T14581	poss	its,partners
R9585	T14581	T14579	pobj	partners,on
R9586	T14582	T14583	det	the,stimulation
R9587	T14583	T14576	attr	stimulation,is
R9588	T14584	T14583	prep	of,stimulation
R9589	T14585	T14586	det	the,pathway
R959	T1687	T1682	pobj	degradation,for
R9590	T14586	T14584	pobj	pathway,of
R9591	T14587	T14586	amod	glycolytic,pathway
R9592	T14588	T14583	cc	and,stimulation
R9593	T14589	T14583	conj	production,stimulation
R9594	T14590	T14589	prep	of,production
R9595	T14591	T14590	pobj	ATP,of
R9596	T14592	T14576	punct	.,is
R9597	T14594	T14595	det	The,pathway
R9598	T14595	T14597	nsubj	pathway,is
R9599	T14596	T14595	amod	glycolytic,pathway
R96	T335	T336	det	a,model
R960	T1688	T1687	compound	protein,degradation
R9600	T14598	T14597	acomp	critical,is
R9601	T14599	T14600	aux	to,fuel
R9602	T14600	T14597	advcl	fuel,is
R9603	T14601	T14602	det	the,pump
R9604	T14602	T14600	dobj	pump,fuel
R9605	T14603	T14602	amod	constitutive,pump
R9606	T14604	T14605	nmod	Na+,K+
R9607	T14605	T14607	compound	K+,ATPase
R9608	T14606	T14605	punct	/,K+
R9609	T14607	T14602	compound	ATPase,pump
R961	T1689	T1690	punct	[,21
R9610	T14608	T14607	punct	-,ATPase
R9611	T14609	T14610	aux	to,maintain
R9612	T14610	T14600	advcl	maintain,fuel
R9613	T14611	T14612	det	the,current
R9614	T14612	T14610	dobj	current,maintain
R9615	T14613	T14612	amod	dark,current
R9616	T14614	T14612	prep	between,current
R9617	T14615	T14616	det	the,compartments
R9618	T14616	T14614	pobj	compartments,between
R9619	T14617	T14616	amod	inner,compartments
R962	T1690	T1652	parataxis	21,required
R9620	T14618	T14617	cc	and,inner
R9621	T14619	T14617	conj	outer,inner
R9622	T14620	T14616	compound	segment,compartments
R9623	T14621	T14616	prep	of,compartments
R9624	T14622	T14623	amod	photosensory,neurons
R9625	T14623	T14621	pobj	neurons,of
R9626	T14624	T14597	punct	.,is
R9627	T14626	T14627	det	A,deficit
R9628	T14627	T14628	nsubj	deficit,disturbs
R9629	T14629	T14627	punct	(,deficit
R963	T1691	T1690	punct	],21
R9630	T14630	T14627	appos	haploinsufficiency,deficit
R9631	T14631	T14627	punct	),deficit
R9632	T14632	T14627	prep	in,deficit
R9633	T14633	T14632	pobj	RanBP2,in
R9634	T14634	T14635	det	the,equilibrium
R9635	T14635	T14628	dobj	equilibrium,disturbs
R9636	T14636	T14635	prep	between,equilibrium
R9637	T14637	T14636	pobj	RanBP2,between
R9638	T14638	T14637	punct	", ",RanBP2
R9639	T14639	T14637	conj	HKI,RanBP2
R964	T1692	T1652	punct	.,required
R9640	T14640	T14639	punct	", ",HKI
R9641	T14641	T14639	cc	and,HKI
R9642	T14642	T14639	conj	Cox11,HKI
R9643	T14643	T14628	punct	.,disturbs
R9644	T14645	T14646	det	This,event
R9645	T14646	T14648	nsubj	event,promotes
R9646	T14647	T14646	amod	pathophysiological,event
R9647	T14649	T14650	det	the,destabilization
R9648	T14650	T14648	dobj	destabilization,promotes
R9649	T14651	T14650	cc	and,destabilization
R965	T1694	T1695	nsubj	Parkin,has
R9650	T14652	T14650	conj	degradation,destabilization
R9651	T14653	T14650	prep	of,destabilization
R9652	T14654	T14653	pobj	HKI,of
R9653	T14655	T14650	cc	and,destabilization
R9654	T14656	T14657	det	a,decrease
R9655	T14657	T14650	conj	decrease,destabilization
R9656	T14658	T14657	prep	in,decrease
R9657	T14659	T14660	compound	ATP,production
R9658	T14660	T14658	pobj	production,in
R9659	T14661	T14660	acl	required,production
R966	T1696	T1694	punct	", ",Parkin
R9660	T14662	T14663	aux	to,maintain
R9661	T14663	T14661	advcl	maintain,required
R9662	T14664	T14665	det	the,neurons
R9663	T14665	T14663	dobj	neurons,maintain
R9664	T14666	T14665	compound	depolarization,neurons
R9665	T14667	T14665	compound	state,neurons
R9666	T14668	T14657	punct	", ",decrease
R9667	T14669	T14657	cc	and,decrease
R9668	T14670	T14657	punct	", ",decrease
R9669	T14671	T14672	advmod	hence,reduction
R967	T1697	T1694	prep	like,Parkin
R9670	T14672	T14657	conj	reduction,decrease
R9671	T14673	T14672	punct	", ",reduction
R9672	T14674	T14672	det	a,reduction
R9673	T14675	T14672	prep	in,reduction
R9674	T14676	T14677	det	the,response
R9675	T14677	T14675	pobj	response,in
R9676	T14678	T14677	prep	of,response
R9677	T14679	T14680	amod	receptoral,neurons
R9678	T14680	T14678	pobj	neurons,of
R9679	T14681	T14679	cc	and,receptoral
R968	T1698	T1697	pobj	RanBP2,like
R9680	T14682	T14679	conj	postreceptoral,receptoral
R9681	T14683	T14648	punct	.,promotes
R9682	T14685	T14686	det	A,reduction
R9683	T14686	T14687	nsubj	reduction,modulates
R9684	T14688	T14686	prep	in,reduction
R9685	T14689	T14690	compound	ATP,levels
R9686	T14690	T14688	pobj	levels,in
R9687	T14691	T14687	advmod	also,modulates
R9688	T14692	T14687	advmod	negatively,modulates
R9689	T14693	T14694	compound	HKI,activity
R969	T1699	T1700	punct	[,17
R9690	T14694	T14687	dobj	activity,modulates
R9691	T14695	T14696	punct	/,level
R9692	T14696	T14694	prep	level,activity
R9693	T14697	T14687	punct	.,modulates
R9694	T14699	T14700	amod	Decreased,levels
R9695	T14700	T14701	nsubj	levels,promote
R9696	T14702	T14700	prep	of,levels
R9697	T14703	T14702	pobj	HKI,of
R9698	T14704	T14705	amod	intracellular,hyperglycemia
R9699	T14705	T14701	dobj	hyperglycemia,promote
R97	T336	T334	pobj	model,in
R970	T1700	T1694	parataxis	17,Parkin
R9700	T14706	T14701	cc	and,promote
R9701	T14707	T14701	conj	activate,promote
R9702	T14708	T14709	compound	stress,kinases
R9703	T14709	T14707	dobj	kinases,activate
R9704	T14710	T14709	punct	", ",kinases
R9705	T14711	T14712	dep	which,modulate
R9706	T14712	T14709	relcl	modulate,kinases
R9707	T14713	T14712	advmod	negatively,modulate
R9708	T14714	T14715	det	the,pump
R9709	T14715	T14712	dobj	pump,modulate
R971	T1701	T1700	punct	],17
R9710	T14716	T14717	compound	Na+,K+
R9711	T14717	T14719	compound	K+,ATPase
R9712	T14718	T14717	punct	/,K+
R9713	T14719	T14715	compound	ATPase,pump
R9714	T14720	T14719	punct	-,ATPase
R9715	T14721	T14712	prep	by,modulate
R9716	T14722	T14721	pobj	phosphorylation,by
R9717	T14723	T14701	punct	.,promote
R9718	T14725	T14726	amod	Pathophysiological,pathways
R9719	T14726	T14727	nsubjpass	pathways,represented
R972	T1702	T1695	punct	", ",has
R9720	T14728	T14726	acl	promoted,pathways
R9721	T14729	T14728	agent	by,promoted
R9722	T14730	T14731	compound	RanBP2,haploinsufficiency
R9723	T14731	T14729	pobj	haploinsufficiency,by
R9724	T14732	T14727	auxpass	are,represented
R9725	T14733	T14727	agent	by,represented
R9726	T14734	T14735	compound	dash,lines
R9727	T14735	T14733	pobj	lines,by
R9728	T14736	T14727	punct	.,represented
R9729	T14739	T14738	punct	", ",RIS
R973	T1703	T1704	compound	E3,ligase
R9730	T14740	T14741	nmod	rod,segment
R9731	T14741	T14738	appos	segment,RIS
R9732	T14742	T14741	amod	inner,segment
R9733	T14743	T14738	punct	;,RIS
R9734	T14744	T14738	appos	ROS,RIS
R9735	T14745	T14744	punct	", ",ROS
R9736	T14746	T14747	nmod	rod,segment
R9737	T14747	T14744	appos	segment,ROS
R9738	T14748	T14747	amod	outer,segment
R9739	T14749	T14738	punct	.,RIS
R974	T1704	T1706	compound	ligase,activity
R975	T1705	T1704	punct	-,ligase
R976	T1706	T1695	dobj	activity,has
R977	T1707	T1708	punct	[,22
R978	T1708	T1695	parataxis	22,has
R979	T1709	T1710	punct	–,24
R98	T337	T336	compound	mouse,model
R980	T1710	T1708	prep	24,22
R981	T1711	T1708	punct	],22
R982	T1712	T1695	punct	;,has
R983	T1713	T1695	cc	and,has
R984	T1714	T1715	nsubj	loss,leads
R985	T1715	T1695	conj	leads,has
R986	T1716	T1714	punct	-,loss
R987	T1717	T1714	prep	of,loss
R988	T1718	T1717	punct	-,of
R989	T1719	T1717	pobj	function,of
R99	T338	T316	punct	.,report
R990	T1720	T1714	prep	of,loss
R991	T1721	T1720	pobj	parkin,of
R992	T1722	T1715	prep	to,leads
R993	T1723	T1724	amod	early,onset
R994	T1724	T1725	nmod	onset,Parkinsonism
R995	T1725	T1722	pobj	Parkinsonism,to
R996	T1726	T1727	amod	autosomal,recessive
R997	T1727	T1725	amod	recessive,Parkinsonism
R998	T1728	T1725	amod	juvenile,Parkinsonism
R999	T1729	T1730	punct	[,25

PMC:1626108 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

PMC:1626108 JSON TXT 8 Projects