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PMC:1584416 / 7536-9732 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T7095 10-19 NN denotes Targeting
T7096 20-22 IN denotes of
T7097 23-26 DT denotes the
T7099 27-32 NN denotes Mouse
T7100 33-36 NN denotes Xpd
T7098 37-41 NN denotes Gene
T7101 41-162 sentence denotes (A) Schematic representation of the genomic structure and partial restriction map of the wt and targeted mouse Xpd loci.
T7102 42-43 -LRB- denotes (
T7103 43-44 LS denotes A
T7105 44-45 -RRB- denotes )
T7106 46-55 JJ denotes Schematic
T7104 56-70 NN denotes representation
T7107 71-73 IN denotes of
T7108 74-77 DT denotes the
T7110 78-85 JJ denotes genomic
T7109 86-95 NN denotes structure
T7111 96-99 CC denotes and
T7112 100-107 JJ denotes partial
T7114 108-119 NN denotes restriction
T7113 120-123 NN denotes map
T7115 124-126 IN denotes of
T7116 127-130 DT denotes the
T7118 131-133 NN denotes wt
T7119 134-137 CC denotes and
T7120 138-146 VBN denotes targeted
T7121 147-152 NN denotes mouse
T7122 153-156 NN denotes Xpd
T7117 157-161 NNS denotes loci
T7123 161-162 . denotes .
T7124 162-287 sentence denotes For the wt Xpd allele, shaded boxes represent coding regions of exons 12 and 19–23; the 3′UTR is represented by an open box.
T7125 163-166 IN denotes For
T7127 167-170 DT denotes the
T7129 171-173 NN denotes wt
T7130 174-177 NN denotes Xpd
T7128 178-184 NN denotes allele
T7131 184-186 , denotes ,
T7132 186-192 JJ denotes shaded
T7133 193-198 NNS denotes boxes
T7134 199-208 VBP denotes represent
T7135 209-215 VBG denotes coding
T7136 216-223 NNS denotes regions
T7137 224-226 IN denotes of
T7138 227-232 NNS denotes exons
T7139 233-235 CD denotes 12
T7140 236-239 CC denotes and
T7141 240-242 CD denotes 19
T7142 242-243 SYM denotes –
T7143 243-245 CD denotes 23
T7144 245-246 : denotes ;
T7145 247-250 DT denotes the
T7147 251-252 CD denotes 3
T7148 252-253 SYM denotes ′
T7146 253-256 NN denotes UTR
T7149 257-259 VBZ denotes is
T7126 260-271 VBN denotes represented
T7150 272-274 IN denotes by
T7151 275-277 DT denotes an
T7153 278-282 JJ denotes open
T7152 283-286 NN denotes box
T7154 286-287 . denotes .
T7155 287-375 sentence denotes TGA indicates the translational stop codon; PolyA indicates the polyadenylation signal.
T7156 288-291 NN denotes TGA
T7157 292-301 VBZ denotes indicates
T7159 302-305 DT denotes the
T7161 306-319 JJ denotes translational
T7162 320-324 NN denotes stop
T7160 325-330 NN denotes codon
T7163 330-331 : denotes ;
T7164 332-337 NN denotes PolyA
T7158 338-347 VBZ denotes indicates
T7165 348-351 DT denotes the
T7167 352-367 NN denotes polyadenylation
T7166 368-374 NN denotes signal
T7168 374-375 . denotes .
T7169 375-567 sentence denotes For the XpdTTD targeted allele, the 194–base pair (bp) human XPD cDNA fragment fused to exon 22 is indicated as a striped box including the TTD (R722W) mutation indicated by a vertical arrow.
T7170 376-379 IN denotes For
T7172 380-383 DT denotes the
T7174 384-390 NN denotes XpdTTD
T7175 391-399 VBN denotes targeted
T7173 400-406 NN denotes allele
T7176 406-408 , denotes ,
T7177 408-411 DT denotes the
T7179 412-415 CD denotes 194
T7181 415-416 HYPH denotes –
T7182 416-420 NN denotes base
T7180 421-425 NN denotes pair
T7183 426-427 -LRB- denotes (
T7184 427-429 NN denotes bp
T7185 429-430 -RRB- denotes )
T7186 431-436 JJ denotes human
T7187 437-440 NN denotes XPD
T7188 441-445 NN denotes cDNA
T7178 446-454 NN denotes fragment
T7189 455-460 VBN denotes fused
T7190 461-463 IN denotes to
T7191 464-468 NN denotes exon
T7192 469-471 CD denotes 22
T7193 472-474 VBZ denotes is
T7171 475-484 VBN denotes indicated
T7194 485-487 IN denotes as
T7195 488-489 DT denotes a
T7197 490-497 VBN denotes striped
T7196 498-501 NN denotes box
T7198 502-511 VBG denotes including
T7199 512-515 DT denotes the
T7201 516-519 NN denotes TTD
T7202 520-521 -LRB- denotes (
T7203 521-526 NN denotes R722W
T7204 526-527 -RRB- denotes )
T7200 528-536 NN denotes mutation
T7205 537-546 VBN denotes indicated
T7206 547-549 IN denotes by
T7207 550-551 DT denotes a
T7209 552-560 JJ denotes vertical
T7208 561-566 NN denotes arrow
T7210 566-567 . denotes .
T7211 567-739 sentence denotes Chicken β-globin exons 2 and 3 including the 3′UTR are indicated as black boxes with corresponding Roman numerals followed by the β-globin polyadenylation signal (PolyA*).
T7212 568-575 NN denotes Chicken
T7214 576-577 NN denotes β
T7216 577-578 HYPH denotes -
T7215 578-584 NN denotes globin
T7217 585-590 NNS denotes exons
T7213 591-592 CD denotes 2
T7219 593-596 CC denotes and
T7220 597-598 CD denotes 3
T7221 599-608 VBG denotes including
T7222 609-612 DT denotes the
T7224 613-614 CD denotes 3
T7225 614-615 HYPH denotes ′
T7223 615-618 NN denotes UTR
T7226 619-622 VBP denotes are
T7218 623-632 VBN denotes indicated
T7227 633-635 IN denotes as
T7228 636-641 JJ denotes black
T7229 642-647 NNS denotes boxes
T7230 648-652 IN denotes with
T7231 653-666 VBG denotes corresponding
T7233 667-672 NNP denotes Roman
T7232 673-681 NNS denotes numerals
T7234 682-690 VBN denotes followed
T7235 691-693 IN denotes by
T7236 694-697 DT denotes the
T7238 698-699 NN denotes β
T7240 699-700 HYPH denotes -
T7239 700-706 NN denotes globin
T7241 707-722 NN denotes polyadenylation
T7237 723-729 NN denotes signal
T7242 730-731 -LRB- denotes (
T7243 731-737 NN denotes PolyA*
T7244 737-738 -RRB- denotes )
T7245 738-739 . denotes .
T7246 739-900 sentence denotes For the Xpd†XP and Xpd†XPCS targeted alleles, vertical arrows indicate XPCS (G602D-encoding) and XP (R683W-encoding) mutations in exons 19 and 22, respectively.
T7247 740-743 IN denotes For
T7249 744-747 DT denotes the
T7251 748-754 NN denotes Xpd†XP
T7252 755-758 CC denotes and
T7253 759-767 NN denotes Xpd†XPCS
T7254 768-776 VBN denotes targeted
T7250 777-784 NNS denotes alleles
T7255 784-786 , denotes ,
T7256 786-794 JJ denotes vertical
T7257 795-801 NNS denotes arrows
T7248 802-810 VBP denotes indicate
T7258 811-815 NN denotes XPCS
T7260 816-817 -LRB- denotes (
T7262 817-822 NN denotes G602D
T7263 822-823 HYPH denotes -
T7261 823-831 VBG denotes encoding
T7264 831-832 -RRB- denotes )
T7265 833-836 CC denotes and
T7266 837-839 NN denotes XP
T7267 840-841 -LRB- denotes (
T7269 841-846 NN denotes R683W
T7270 846-847 HYPH denotes -
T7268 847-855 VBG denotes encoding
T7271 855-856 -RRB- denotes )
T7259 857-866 NNS denotes mutations
T7272 867-869 IN denotes in
T7273 870-875 NNS denotes exons
T7274 876-878 CD denotes 19
T7275 879-882 CC denotes and
T7276 883-885 CD denotes 22
T7277 885-887 , denotes ,
T7278 887-899 RB denotes respectively
T7279 899-900 . denotes .
T7280 900-993 sentence denotes The unique 3′ probe located outside the targeting construct is marked by a thick black line.
T7281 901-904 DT denotes The
T7283 905-911 JJ denotes unique
T7284 912-913 CD denotes 3
T7285 913-914 SYM denotes ′
T7282 915-920 NN denotes probe
T7287 921-928 VBN denotes located
T7288 929-936 IN denotes outside
T7289 937-940 DT denotes the
T7291 941-950 VBG denotes targeting
T7290 951-960 NN denotes construct
T7292 961-963 VBZ denotes is
T7286 964-970 VBN denotes marked
T7293 971-973 IN denotes by
T7294 974-975 DT denotes a
T7296 976-981 JJ denotes thick
T7297 982-987 JJ denotes black
T7295 988-992 NN denotes line
T7298 992-993 . denotes .
T7299 993-1073 sentence denotes Restriction sites: B, BamHI; C, ClaI; E, EcoRI; H, HindIII; Hp, HpaI; Sf, SfiI.
T7300 994-1005 NN denotes Restriction
T7301 1006-1011 NNS denotes sites
T7302 1011-1013 : denotes :
T7303 1013-1014 NN denotes B
T7304 1014-1016 , denotes ,
T7305 1016-1021 NN denotes BamHI
T7306 1021-1022 : denotes ;
T7307 1023-1024 NN denotes C
T7308 1024-1026 , denotes ,
T7309 1026-1030 NN denotes ClaI
T7310 1030-1031 : denotes ;
T7311 1032-1033 NN denotes E
T7312 1033-1035 , denotes ,
T7313 1035-1040 NN denotes EcoRI
T7314 1040-1041 : denotes ;
T7315 1042-1043 NN denotes H
T7316 1043-1045 , denotes ,
T7317 1045-1052 NN denotes HindIII
T7318 1052-1053 : denotes ;
T7319 1054-1056 NN denotes Hp
T7320 1056-1058 , denotes ,
T7321 1058-1062 NN denotes HpaI
T7322 1062-1063 : denotes ;
T7323 1064-1066 NN denotes Sf
T7324 1066-1068 , denotes ,
T7325 1068-1072 NN denotes SfiI
T7326 1072-1073 . denotes .
T7327 1073-1251 sentence denotes (B) Southern blot analysis of EcoRI-digested genomic DNA from wt, Xpd†XPCS/wt, and Xpd†XP/wt recombinant embryonic stem cell clones hybridised with the 3′ probe depicted in (A).
T7328 1074-1075 -LRB- denotes (
T7329 1075-1076 LS denotes B
T7331 1076-1077 -RRB- denotes )
T7332 1078-1086 NNP denotes Southern
T7333 1087-1091 NN denotes blot
T7330 1092-1100 NN denotes analysis
T7334 1101-1103 IN denotes of
T7335 1104-1109 NN denotes EcoRI
T7337 1109-1110 HYPH denotes -
T7336 1110-1118 VBN denotes digested
T7339 1119-1126 JJ denotes genomic
T7338 1127-1130 NN denotes DNA
T7340 1131-1135 IN denotes from
T7341 1136-1138 NN denotes wt
T7343 1138-1140 , denotes ,
T7344 1140-1148 NN denotes Xpd†XPCS
T7346 1148-1149 HYPH denotes /
T7345 1149-1151 NN denotes wt
T7347 1151-1153 , denotes ,
T7348 1153-1156 CC denotes and
T7349 1157-1163 NN denotes Xpd†XP
T7351 1163-1164 HYPH denotes /
T7350 1164-1166 NN denotes wt
T7352 1167-1178 JJ denotes recombinant
T7353 1179-1188 JJ denotes embryonic
T7355 1189-1193 NN denotes stem
T7354 1194-1198 NN denotes cell
T7342 1199-1205 NNS denotes clones
T7356 1206-1216 VBN denotes hybridised
T7357 1217-1221 IN denotes with
T7358 1222-1225 DT denotes the
T7360 1226-1227 CD denotes 3
T7361 1227-1228 SYM denotes ′
T7359 1229-1234 NN denotes probe
T7362 1235-1243 VBN denotes depicted
T7363 1244-1246 IN denotes in
T7364 1247-1248 -LRB- denotes (
T7365 1248-1249 NN denotes A
T7366 1249-1250 -RRB- denotes )
T7367 1250-1251 . denotes .
T7368 1251-1377 sentence denotes The wt allele yields a 6.5-kilobase (kb) fragment, whereas both targeted Xpd†XP and Xpd†XPCS alleles yield a 5.1-kb fragment.
T7369 1252-1255 DT denotes The
T7371 1256-1258 NN denotes wt
T7370 1259-1265 NN denotes allele
T7372 1266-1272 VBZ denotes yields
T7373 1273-1274 DT denotes a
T7375 1275-1278 CD denotes 6.5
T7377 1278-1279 HYPH denotes -
T7376 1279-1287 NN denotes kilobase
T7378 1288-1289 -LRB- denotes (
T7379 1289-1291 NN denotes kb
T7380 1291-1292 -RRB- denotes )
T7374 1293-1301 NN denotes fragment
T7381 1301-1303 , denotes ,
T7382 1303-1310 IN denotes whereas
T7384 1311-1315 CC denotes both
T7386 1316-1324 VBN denotes targeted
T7387 1325-1331 NN denotes Xpd†XP
T7388 1332-1335 CC denotes and
T7389 1336-1344 NN denotes Xpd†XPCS
T7385 1345-1352 NNS denotes alleles
T7383 1353-1358 VBP denotes yield
T7390 1359-1360 DT denotes a
T7392 1361-1364 CD denotes 5.1
T7394 1364-1365 HYPH denotes -
T7393 1365-1367 NN denotes kb
T7391 1368-1376 NN denotes fragment
T7395 1376-1377 . denotes .
T7396 1377-1528 sentence denotes (C) Genotyping of wt and targeted alleles by PCR using primers F2, R1, and mR as indicated in (A) yields fragments of 399 bp and 468 bp, respectively.
T7397 1378-1379 -LRB- denotes (
T7398 1379-1380 LS denotes C
T7400 1380-1381 -RRB- denotes )
T7399 1382-1392 NN denotes Genotyping
T7401 1393-1395 IN denotes of
T7402 1396-1398 NN denotes wt
T7404 1399-1402 CC denotes and
T7405 1403-1411 VBN denotes targeted
T7403 1412-1419 NNS denotes alleles
T7406 1420-1422 IN denotes by
T7407 1423-1426 NN denotes PCR
T7408 1427-1432 VBG denotes using
T7409 1433-1440 NNS denotes primers
T7410 1441-1443 NN denotes F2
T7411 1443-1445 , denotes ,
T7412 1445-1447 NN denotes R1
T7413 1447-1449 , denotes ,
T7414 1449-1452 CC denotes and
T7415 1453-1455 NN denotes mR
T7416 1456-1458 IN denotes as
T7417 1459-1468 VBN denotes indicated
T7418 1469-1471 IN denotes in
T7419 1472-1473 -LRB- denotes (
T7420 1473-1474 LS denotes A
T7422 1474-1475 -RRB- denotes )
T7423 1476-1482 VBZ denotes yields
T7421 1483-1492 NNS denotes fragments
T7424 1493-1495 IN denotes of
T7425 1496-1499 CD denotes 399
T7426 1500-1502 NN denotes bp
T7427 1503-1506 CC denotes and
T7428 1507-1510 CD denotes 468
T7429 1511-1513 NN denotes bp
T7430 1513-1515 , denotes ,
T7431 1515-1527 RB denotes respectively
T7432 1527-1528 . denotes .
T7433 1528-1772 sentence denotes (D) RT-PCR detection of mRNA expression originating from the targeted †XP and †XPCS alleles in embryonic stem cell clones using primers F1 (hybridising outside the targeting construct) and mR as indicated in (A) results in a 1,416-bp fragment.
T7434 1529-1530 -LRB- denotes (
T7435 1530-1531 LS denotes D
T7437 1531-1532 -RRB- denotes )
T7438 1533-1535 NN denotes RT
T7440 1535-1536 HYPH denotes -
T7439 1536-1539 NN denotes PCR
T7441 1540-1549 NN denotes detection
T7442 1550-1552 IN denotes of
T7443 1553-1557 NN denotes mRNA
T7444 1558-1568 NN denotes expression
T7445 1569-1580 VBG denotes originating
T7446 1581-1585 IN denotes from
T7447 1586-1589 DT denotes the
T7449 1590-1598 VBN denotes targeted
T7450 1599-1602 NN denotes †XP
T7451 1603-1606 CC denotes and
T7452 1607-1612 NN denotes †XPCS
T7448 1613-1620 NNS denotes alleles
T7453 1621-1623 IN denotes in
T7454 1624-1633 JJ denotes embryonic
T7456 1634-1638 NN denotes stem
T7455 1639-1643 NN denotes cell
T7457 1644-1650 NNS denotes clones
T7458 1651-1656 VBG denotes using
T7459 1657-1664 NNS denotes primers
T7460 1665-1667 NN denotes F1
T7461 1668-1669 -LRB- denotes (
T7462 1669-1680 VBG denotes hybridising
T7463 1681-1688 IN denotes outside
T7464 1689-1692 DT denotes the
T7466 1693-1702 VBG denotes targeting
T7465 1703-1712 NN denotes construct
T7467 1712-1713 -RRB- denotes )
T7468 1714-1717 CC denotes and
T7469 1718-1720 NN denotes mR
T7470 1721-1723 IN denotes as
T7471 1724-1733 VBN denotes indicated
T7472 1734-1736 IN denotes in
T7473 1737-1738 -LRB- denotes (
T7474 1738-1739 NN denotes A
T7475 1739-1740 -RRB- denotes )
T7436 1741-1748 VBZ denotes results
T7476 1749-1751 IN denotes in
T7477 1752-1753 DT denotes a
T7479 1754-1759 CD denotes 1,416
T7481 1759-1760 HYPH denotes -
T7480 1760-1762 NN denotes bp
T7478 1763-1771 NN denotes fragment
T7482 1771-1772 . denotes .
T7483 1772-1964 sentence denotes (E) Northern blot analysis of total RNA isolated from testis of homozygous wt and XpdTTD/TTD, heterozygous Xpd†XPCS/wt and XpdTTD/wt, and compound heterozygous Xpd†XPCS/TTD mice as indicated.
T7484 1773-1774 -LRB- denotes (
T7485 1774-1775 LS denotes E
T7487 1775-1776 -RRB- denotes )
T7488 1777-1785 NNP denotes Northern
T7489 1786-1790 NN denotes blot
T7486 1791-1799 NN denotes analysis
T7490 1800-1802 IN denotes of
T7491 1803-1808 JJ denotes total
T7492 1809-1812 NN denotes RNA
T7493 1813-1821 VBN denotes isolated
T7494 1822-1826 IN denotes from
T7495 1827-1833 NN denotes testis
T7496 1834-1836 IN denotes of
T7497 1837-1847 JJ denotes homozygous
T7498 1848-1850 NN denotes wt
T7500 1851-1854 CC denotes and
T7501 1855-1861 NN denotes XpdTTD
T7503 1861-1862 HYPH denotes /
T7502 1862-1865 NN denotes TTD
T7504 1865-1867 , denotes ,
T7505 1867-1879 JJ denotes heterozygous
T7507 1880-1888 NN denotes Xpd†XPCS
T7508 1888-1889 HYPH denotes /
T7506 1889-1891 NN denotes wt
T7509 1892-1895 CC denotes and
T7510 1896-1902 NN denotes XpdTTD
T7512 1902-1903 HYPH denotes /
T7511 1903-1905 NN denotes wt
T7513 1905-1907 , denotes ,
T7514 1907-1910 CC denotes and
T7515 1911-1919 JJ denotes compound
T7517 1920-1932 JJ denotes heterozygous
T7518 1933-1941 NN denotes Xpd†XPCS
T7519 1941-1942 HYPH denotes /
T7516 1942-1945 NN denotes TTD
T7499 1946-1950 NNS denotes mice
T7520 1951-1953 IN denotes as
T7521 1954-1963 VBN denotes indicated
T7522 1963-1964 . denotes .
T7523 1964-2102 sentence denotes Hybridisation with a 1.4-kb mouse Xpd cDNA probe detects mRNAs of 4, 3.3, and 2.7 kb from wt, Xpd†XPCS, and XpdTTD alleles, respectively.
T7524 1965-1978 NN denotes Hybridisation
T7526 1979-1983 IN denotes with
T7527 1984-1985 DT denotes a
T7529 1986-1989 CD denotes 1.4
T7531 1989-1990 HYPH denotes -
T7530 1990-1992 NN denotes kb
T7532 1993-1998 NN denotes mouse
T7533 1999-2002 NN denotes Xpd
T7534 2003-2007 NN denotes cDNA
T7528 2008-2013 NN denotes probe
T7525 2014-2021 VBZ denotes detects
T7535 2022-2027 NNS denotes mRNAs
T7536 2028-2030 IN denotes of
T7537 2031-2032 CD denotes 4
T7539 2032-2034 , denotes ,
T7540 2034-2037 CD denotes 3.3
T7541 2037-2039 , denotes ,
T7542 2039-2042 CC denotes and
T7543 2043-2046 CD denotes 2.7
T7538 2047-2049 NN denotes kb
T7544 2050-2054 IN denotes from
T7545 2055-2057 NN denotes wt
T7547 2057-2059 , denotes ,
T7548 2059-2067 NN denotes Xpd†XPCS
T7549 2067-2069 , denotes ,
T7550 2069-2072 CC denotes and
T7551 2073-2079 NN denotes XpdTTD
T7546 2080-2087 NNS denotes alleles
T7552 2087-2089 , denotes ,
T7553 2089-2101 RB denotes respectively
T7554 2101-2102 . denotes .
T7555 2102-2196 sentence denotes An ethidium bromide (EtBr)–stained gel showing the amount of total RNA loaded is shown below.
T7556 2103-2105 DT denotes An
T7558 2106-2114 NN denotes ethidium
T7559 2115-2122 NN denotes bromide
T7561 2123-2124 -LRB- denotes (
T7562 2124-2128 NN denotes EtBr
T7563 2128-2129 -RRB- denotes )
T7564 2129-2130 HYPH denotes –
T7560 2130-2137 VBN denotes stained
T7557 2138-2141 NN denotes gel
T7566 2142-2149 VBG denotes showing
T7567 2150-2153 DT denotes the
T7568 2154-2160 NN denotes amount
T7569 2161-2163 IN denotes of
T7570 2164-2169 JJ denotes total
T7571 2170-2173 NN denotes RNA
T7572 2174-2180 VBN denotes loaded
T7573 2181-2183 VBZ denotes is
T7565 2184-2189 VBN denotes shown
T7574 2190-2195 RB denotes below
T7575 2195-2196 . denotes .
R4726 T7096 T7095 prep of,Targeting
R4727 T7097 T7098 det the,Gene
R4728 T7098 T7096 pobj Gene,of
R4729 T7099 T7098 compound Mouse,Gene
R4730 T7100 T7098 compound Xpd,Gene
R4731 T7102 T7103 punct (,A
R4732 T7103 T7104 meta A,representation
R4733 T7105 T7103 punct ),A
R4734 T7106 T7104 amod Schematic,representation
R4735 T7107 T7104 prep of,representation
R4736 T7108 T7109 det the,structure
R4737 T7109 T7107 pobj structure,of
R4738 T7110 T7109 amod genomic,structure
R4739 T7111 T7104 cc and,representation
R4740 T7112 T7113 amod partial,map
R4741 T7113 T7104 conj map,representation
R4742 T7114 T7113 compound restriction,map
R4743 T7115 T7113 prep of,map
R4744 T7116 T7117 det the,loci
R4745 T7117 T7115 pobj loci,of
R4746 T7118 T7117 nmod wt,loci
R4747 T7119 T7118 cc and,wt
R4748 T7120 T7118 conj targeted,wt
R4749 T7121 T7117 compound mouse,loci
R4750 T7122 T7117 compound Xpd,loci
R4751 T7123 T7113 punct .,map
R4752 T7125 T7126 prep For,represented
R4753 T7127 T7128 det the,allele
R4754 T7128 T7125 pobj allele,For
R4755 T7129 T7128 compound wt,allele
R4756 T7130 T7128 compound Xpd,allele
R4757 T7131 T7126 punct ", ",represented
R4758 T7132 T7133 amod shaded,boxes
R4759 T7133 T7134 nsubj boxes,represent
R4760 T7134 T7126 ccomp represent,represented
R4761 T7135 T7136 amod coding,regions
R4762 T7136 T7134 dobj regions,represent
R4763 T7137 T7136 prep of,regions
R4764 T7138 T7139 nmod exons,12
R4765 T7139 T7137 pobj 12,of
R4766 T7140 T7139 cc and,12
R4767 T7141 T7139 conj 19,12
R4768 T7142 T7143 punct –,23
R4769 T7143 T7141 prep 23,19
R4770 T7144 T7126 punct ;,represented
R4771 T7145 T7146 det the,UTR
R4772 T7146 T7126 nsubjpass UTR,represented
R4773 T7147 T7146 nummod 3,UTR
R4774 T7148 T7147 punct ′,3
R4775 T7149 T7126 auxpass is,represented
R4776 T7150 T7126 agent by,represented
R4777 T7151 T7152 det an,box
R4778 T7152 T7150 pobj box,by
R4779 T7153 T7152 amod open,box
R4780 T7154 T7126 punct .,represented
R4781 T7156 T7157 nsubj TGA,indicates
R4782 T7157 T7158 ccomp indicates,indicates
R4783 T7159 T7160 det the,codon
R4784 T7160 T7157 dobj codon,indicates
R4785 T7161 T7160 amod translational,codon
R4786 T7162 T7160 compound stop,codon
R4787 T7163 T7158 punct ;,indicates
R4788 T7164 T7158 nsubj PolyA,indicates
R4789 T7165 T7166 det the,signal
R4790 T7166 T7158 dobj signal,indicates
R4791 T7167 T7166 compound polyadenylation,signal
R4792 T7168 T7158 punct .,indicates
R4793 T7170 T7171 prep For,indicated
R4794 T7172 T7173 det the,allele
R4795 T7173 T7170 pobj allele,For
R4796 T7174 T7173 nmod XpdTTD,allele
R4797 T7175 T7173 amod targeted,allele
R4798 T7176 T7171 punct ", ",indicated
R4799 T7177 T7178 det the,fragment
R4800 T7178 T7171 nsubjpass fragment,indicated
R4801 T7179 T7180 nummod 194,pair
R4802 T7180 T7178 nmod pair,fragment
R4803 T7181 T7180 punct –,pair
R4804 T7182 T7180 nmod base,pair
R4805 T7183 T7180 punct (,pair
R4806 T7184 T7180 appos bp,pair
R4807 T7185 T7178 punct ),fragment
R4808 T7186 T7178 amod human,fragment
R4809 T7187 T7178 compound XPD,fragment
R4810 T7188 T7178 compound cDNA,fragment
R4811 T7189 T7178 acl fused,fragment
R4812 T7190 T7189 prep to,fused
R4813 T7191 T7190 pobj exon,to
R4814 T7192 T7191 nummod 22,exon
R4815 T7193 T7171 auxpass is,indicated
R4816 T7194 T7171 prep as,indicated
R4817 T7195 T7196 det a,box
R4818 T7196 T7194 pobj box,as
R4819 T7197 T7196 amod striped,box
R4820 T7198 T7171 prep including,indicated
R4821 T7199 T7200 det the,mutation
R4822 T7200 T7198 pobj mutation,including
R4823 T7201 T7200 nmod TTD,mutation
R4824 T7202 T7203 punct (,R722W
R4825 T7203 T7201 parataxis R722W,TTD
R4826 T7204 T7203 punct ),R722W
R4827 T7205 T7200 acl indicated,mutation
R4828 T7206 T7205 agent by,indicated
R4829 T7207 T7208 det a,arrow
R4830 T7208 T7206 pobj arrow,by
R4831 T7209 T7208 amod vertical,arrow
R4832 T7210 T7171 punct .,indicated
R4833 T7212 T7213 nmod Chicken,2
R4834 T7213 T7218 nsubjpass 2,indicated
R4835 T7214 T7215 nmod β,globin
R4836 T7215 T7213 nmod globin,2
R4837 T7216 T7215 punct -,globin
R4838 T7217 T7213 nmod exons,2
R4839 T7219 T7213 cc and,2
R4840 T7220 T7213 conj 3,2
R4841 T7221 T7213 prep including,2
R4842 T7222 T7223 det the,UTR
R4843 T7223 T7221 pobj UTR,including
R4844 T7224 T7223 nummod 3,UTR
R4845 T7225 T7224 punct ′,3
R4846 T7226 T7218 auxpass are,indicated
R4847 T7227 T7218 prep as,indicated
R4848 T7228 T7229 amod black,boxes
R4849 T7229 T7227 pobj boxes,as
R4850 T7230 T7229 prep with,boxes
R4851 T7231 T7232 amod corresponding,numerals
R4852 T7232 T7230 pobj numerals,with
R4853 T7233 T7232 compound Roman,numerals
R4854 T7234 T7232 acl followed,numerals
R4855 T7235 T7234 agent by,followed
R4856 T7236 T7237 det the,signal
R4857 T7237 T7235 pobj signal,by
R4858 T7238 T7239 compound β,globin
R4859 T7239 T7237 compound globin,signal
R4860 T7240 T7239 punct -,globin
R4861 T7241 T7237 compound polyadenylation,signal
R4862 T7242 T7243 punct (,PolyA*
R4863 T7243 T7237 parataxis PolyA*,signal
R4864 T7244 T7243 punct ),PolyA*
R4865 T7245 T7218 punct .,indicated
R4866 T7247 T7248 prep For,indicate
R4867 T7249 T7250 det the,alleles
R4868 T7250 T7247 pobj alleles,For
R4869 T7251 T7250 nmod Xpd†XP,alleles
R4870 T7252 T7251 cc and,Xpd†XP
R4871 T7253 T7251 conj Xpd†XPCS,Xpd†XP
R4872 T7254 T7250 amod targeted,alleles
R4873 T7255 T7248 punct ", ",indicate
R4874 T7256 T7257 amod vertical,arrows
R4875 T7257 T7248 nsubj arrows,indicate
R4876 T7258 T7259 nmod XPCS,mutations
R4877 T7259 T7248 dobj mutations,indicate
R4878 T7260 T7261 punct (,encoding
R4879 T7261 T7258 parataxis encoding,XPCS
R4880 T7262 T7261 npadvmod G602D,encoding
R4881 T7263 T7261 punct -,encoding
R4882 T7264 T7261 punct ),encoding
R4883 T7265 T7258 cc and,XPCS
R4884 T7266 T7258 conj XP,XPCS
R4885 T7267 T7268 punct (,encoding
R4886 T7268 T7266 parataxis encoding,XP
R4887 T7269 T7268 npadvmod R683W,encoding
R4888 T7270 T7268 punct -,encoding
R4889 T7271 T7268 punct ),encoding
R4890 T7272 T7259 prep in,mutations
R4891 T7273 T7274 nmod exons,19
R4892 T7274 T7272 pobj 19,in
R4893 T7275 T7274 cc and,19
R4894 T7276 T7274 conj 22,19
R4895 T7277 T7248 punct ", ",indicate
R4896 T7278 T7248 advmod respectively,indicate
R4897 T7279 T7248 punct .,indicate
R4898 T7281 T7282 det The,probe
R4899 T7282 T7286 nsubjpass probe,marked
R4900 T7283 T7282 amod unique,probe
R4901 T7284 T7282 nummod 3,probe
R4902 T7285 T7284 punct ′,3
R4903 T7287 T7282 acl located,probe
R4904 T7288 T7287 prep outside,located
R4905 T7289 T7290 det the,construct
R4906 T7290 T7288 pobj construct,outside
R4907 T7291 T7290 amod targeting,construct
R4908 T7292 T7286 auxpass is,marked
R4909 T7293 T7286 agent by,marked
R4910 T7294 T7295 det a,line
R4911 T7295 T7293 pobj line,by
R4912 T7296 T7295 amod thick,line
R4913 T7297 T7295 amod black,line
R4914 T7298 T7286 punct .,marked
R4915 T7300 T7301 compound Restriction,sites
R4916 T7302 T7301 punct : ,sites
R4917 T7303 T7301 appos B,sites
R4918 T7304 T7303 punct ", ",B
R4919 T7305 T7303 appos BamHI,B
R4920 T7306 T7303 punct ;,B
R4921 T7307 T7303 appos C,B
R4922 T7308 T7307 punct ", ",C
R4923 T7309 T7307 appos ClaI,C
R4924 T7310 T7303 punct ;,B
R4925 T7311 T7303 appos E,B
R4926 T7312 T7311 punct ", ",E
R4927 T7313 T7311 appos EcoRI,E
R4928 T7314 T7303 punct ;,B
R4929 T7315 T7303 appos H,B
R4930 T7316 T7315 punct ", ",H
R4931 T7317 T7315 appos HindIII,H
R4932 T7318 T7303 punct ;,B
R4933 T7319 T7303 appos Hp,B
R4934 T7320 T7319 punct ", ",Hp
R4935 T7321 T7319 appos HpaI,Hp
R4936 T7322 T7303 punct ;,B
R4937 T7323 T7303 appos Sf,B
R4938 T7324 T7323 punct ", ",Sf
R4939 T7325 T7323 appos SfiI,Sf
R4940 T7326 T7301 punct .,sites
R4941 T7328 T7329 punct (,B
R4942 T7329 T7330 meta B,analysis
R4943 T7331 T7329 punct ),B
R4944 T7332 T7333 compound Southern,blot
R4945 T7333 T7330 compound blot,analysis
R4946 T7334 T7330 prep of,analysis
R4947 T7335 T7336 npadvmod EcoRI,digested
R4948 T7336 T7338 amod digested,DNA
R4949 T7337 T7336 punct -,digested
R4950 T7338 T7334 pobj DNA,of
R4951 T7339 T7338 amod genomic,DNA
R4952 T7340 T7338 prep from,DNA
R4953 T7341 T7342 nmod wt,clones
R4954 T7342 T7340 pobj clones,from
R4955 T7343 T7341 punct ", ",wt
R4956 T7344 T7345 compound Xpd†XPCS,wt
R4957 T7345 T7341 conj wt,wt
R4958 T7346 T7345 punct /,wt
R4959 T7347 T7345 punct ", ",wt
R4960 T7348 T7345 cc and,wt
R4961 T7349 T7350 compound Xpd†XP,wt
R4962 T7350 T7345 conj wt,wt
R4963 T7351 T7350 punct /,wt
R4964 T7352 T7342 amod recombinant,clones
R4965 T7353 T7354 amod embryonic,cell
R4966 T7354 T7342 compound cell,clones
R4967 T7355 T7354 compound stem,cell
R4968 T7356 T7342 acl hybridised,clones
R4969 T7357 T7356 prep with,hybridised
R4970 T7358 T7359 det the,probe
R4971 T7359 T7357 pobj probe,with
R4972 T7360 T7359 nummod 3,probe
R4973 T7361 T7360 punct ′,3
R4974 T7362 T7359 acl depicted,probe
R4975 T7363 T7362 prep in,depicted
R4976 T7364 T7363 punct (,in
R4977 T7365 T7363 pobj A,in
R4978 T7366 T7330 punct ),analysis
R4979 T7367 T7330 punct .,analysis
R4980 T7369 T7370 det The,allele
R4981 T7370 T7372 nsubj allele,yields
R4982 T7371 T7370 compound wt,allele
R4983 T7373 T7374 det a,fragment
R4984 T7374 T7372 dobj fragment,yields
R4985 T7375 T7376 nummod 6.5,kilobase
R4986 T7376 T7374 nmod kilobase,fragment
R4987 T7377 T7376 punct -,kilobase
R4988 T7378 T7376 punct (,kilobase
R4989 T7379 T7376 appos kb,kilobase
R4990 T7380 T7374 punct ),fragment
R4991 T7381 T7372 punct ", ",yields
R4992 T7382 T7383 mark whereas,yield
R4993 T7383 T7372 advcl yield,yields
R4994 T7384 T7385 preconj both,alleles
R4995 T7385 T7383 nsubj alleles,yield
R4996 T7386 T7385 amod targeted,alleles
R4997 T7387 T7385 nmod Xpd†XP,alleles
R4998 T7388 T7387 cc and,Xpd†XP
R4999 T7389 T7387 conj Xpd†XPCS,Xpd†XP
R5000 T7390 T7391 det a,fragment
R5001 T7391 T7383 dobj fragment,yield
R5002 T7392 T7393 nummod 5.1,kb
R5003 T7393 T7391 compound kb,fragment
R5004 T7394 T7393 punct -,kb
R5005 T7395 T7372 punct .,yields
R5006 T7397 T7398 punct (,C
R5007 T7398 T7399 meta C,Genotyping
R5008 T7400 T7398 punct ),C
R5009 T7401 T7399 prep of,Genotyping
R5010 T7402 T7403 nmod wt,alleles
R5011 T7403 T7401 pobj alleles,of
R5012 T7404 T7402 cc and,wt
R5013 T7405 T7402 conj targeted,wt
R5014 T7406 T7399 prep by,Genotyping
R5015 T7407 T7406 pobj PCR,by
R5016 T7408 T7399 acl using,Genotyping
R5017 T7409 T7408 dobj primers,using
R5018 T7410 T7409 appos F2,primers
R5019 T7411 T7410 punct ", ",F2
R5020 T7412 T7410 conj R1,F2
R5021 T7413 T7412 punct ", ",R1
R5022 T7414 T7412 cc and,R1
R5023 T7415 T7412 conj mR,R1
R5024 T7416 T7417 mark as,indicated
R5025 T7417 T7408 advcl indicated,using
R5026 T7418 T7408 prep in,using
R5027 T7419 T7420 punct (,A
R5028 T7420 T7421 meta A,fragments
R5029 T7421 T7418 pobj fragments,in
R5030 T7422 T7420 punct ),A
R5031 T7423 T7421 nmod yields,fragments
R5032 T7424 T7421 prep of,fragments
R5033 T7425 T7426 nummod 399,bp
R5034 T7426 T7424 pobj bp,of
R5035 T7427 T7426 cc and,bp
R5036 T7428 T7429 nummod 468,bp
R5037 T7429 T7426 conj bp,bp
R5038 T7430 T7408 punct ", ",using
R5039 T7431 T7408 advmod respectively,using
R5040 T7432 T7399 punct .,Genotyping
R5041 T7434 T7435 punct (,D
R5042 T7435 T7436 meta D,results
R5043 T7437 T7435 punct ),D
R5044 T7438 T7439 compound RT,PCR
R5045 T7439 T7441 compound PCR,detection
R5046 T7440 T7439 punct -,PCR
R5047 T7441 T7436 nsubj detection,results
R5048 T7442 T7441 prep of,detection
R5049 T7443 T7444 compound mRNA,expression
R5050 T7444 T7442 pobj expression,of
R5051 T7445 T7444 acl originating,expression
R5052 T7446 T7445 prep from,originating
R5053 T7447 T7448 det the,alleles
R5054 T7448 T7446 pobj alleles,from
R5055 T7449 T7448 amod targeted,alleles
R5056 T7450 T7448 nmod †XP,alleles
R5057 T7451 T7450 cc and,†XP
R5058 T7452 T7450 conj †XPCS,†XP
R5059 T7453 T7441 prep in,detection
R5060 T7454 T7455 amod embryonic,cell
R5061 T7455 T7457 compound cell,clones
R5062 T7456 T7455 compound stem,cell
R5063 T7457 T7453 pobj clones,in
R5064 T7458 T7441 acl using,detection
R5065 T7459 T7458 dobj primers,using
R5066 T7460 T7459 appos F1,primers
R5067 T7461 T7462 punct (,hybridising
R5068 T7462 T7460 parataxis hybridising,F1
R5069 T7463 T7462 prep outside,hybridising
R5070 T7464 T7465 det the,construct
R5071 T7465 T7463 pobj construct,outside
R5072 T7466 T7465 amod targeting,construct
R5073 T7467 T7462 punct ),hybridising
R5074 T7468 T7460 cc and,F1
R5075 T7469 T7460 conj mR,F1
R5076 T7470 T7471 mark as,indicated
R5077 T7471 T7458 advcl indicated,using
R5078 T7472 T7471 prep in,indicated
R5079 T7473 T7472 punct (,in
R5080 T7474 T7472 pobj A,in
R5081 T7475 T7436 punct ),results
R5082 T7476 T7436 prep in,results
R5083 T7477 T7478 det a,fragment
R5084 T7478 T7476 pobj fragment,in
R5085 T7479 T7480 nummod "1,416",bp
R5086 T7480 T7478 compound bp,fragment
R5087 T7481 T7480 punct -,bp
R5088 T7482 T7436 punct .,results
R5089 T7484 T7485 punct (,E
R5090 T7485 T7486 meta E,analysis
R5091 T7487 T7485 punct ),E
R5092 T7488 T7489 compound Northern,blot
R5093 T7489 T7486 compound blot,analysis
R5094 T7490 T7486 prep of,analysis
R5095 T7491 T7492 amod total,RNA
R5096 T7492 T7490 pobj RNA,of
R5097 T7493 T7492 acl isolated,RNA
R5098 T7494 T7493 prep from,isolated
R5099 T7495 T7494 pobj testis,from
R5100 T7496 T7495 prep of,testis
R5101 T7497 T7498 amod homozygous,wt
R5102 T7498 T7499 nmod wt,mice
R5103 T7499 T7496 pobj mice,of
R5104 T7500 T7498 cc and,wt
R5105 T7501 T7502 compound XpdTTD,TTD
R5106 T7502 T7498 conj TTD,wt
R5107 T7503 T7502 punct /,TTD
R5108 T7504 T7498 punct ", ",wt
R5109 T7505 T7506 amod heterozygous,wt
R5110 T7506 T7498 conj wt,wt
R5111 T7507 T7506 compound Xpd†XPCS,wt
R5112 T7508 T7506 punct /,wt
R5113 T7509 T7506 cc and,wt
R5114 T7510 T7511 compound XpdTTD,wt
R5115 T7511 T7506 conj wt,wt
R5116 T7512 T7511 punct /,wt
R5117 T7513 T7506 punct ", ",wt
R5118 T7514 T7506 cc and,wt
R5119 T7515 T7516 amod compound,TTD
R5120 T7516 T7506 conj TTD,wt
R5121 T7517 T7516 amod heterozygous,TTD
R5122 T7518 T7516 compound Xpd†XPCS,TTD
R5123 T7519 T7516 punct /,TTD
R5124 T7520 T7521 mark as,indicated
R5125 T7521 T7493 advcl indicated,isolated
R5126 T7522 T7486 punct .,analysis
R5127 T7524 T7525 nsubj Hybridisation,detects
R5128 T7526 T7524 prep with,Hybridisation
R5129 T7527 T7528 det a,probe
R5130 T7528 T7526 pobj probe,with
R5131 T7529 T7530 nummod 1.4,kb
R5132 T7530 T7528 compound kb,probe
R5133 T7531 T7530 punct -,kb
R5134 T7532 T7528 compound mouse,probe
R5135 T7533 T7528 compound Xpd,probe
R5136 T7534 T7528 compound cDNA,probe
R5137 T7535 T7525 dobj mRNAs,detects
R5138 T7536 T7535 prep of,mRNAs
R5139 T7537 T7538 nummod 4,kb
R5140 T7538 T7536 pobj kb,of
R5141 T7539 T7537 punct ", ",4
R5142 T7540 T7537 conj 3.3,4
R5143 T7541 T7540 punct ", ",3.3
R5144 T7542 T7540 cc and,3.3
R5145 T7543 T7540 conj 2.7,3.3
R5146 T7544 T7535 prep from,mRNAs
R5147 T7545 T7546 nmod wt,alleles
R5148 T7546 T7544 pobj alleles,from
R5149 T7547 T7545 punct ", ",wt
R5150 T7548 T7545 conj Xpd†XPCS,wt
R5151 T7549 T7548 punct ", ",Xpd†XPCS
R5152 T7550 T7548 cc and,Xpd†XPCS
R5153 T7551 T7548 conj XpdTTD,Xpd†XPCS
R5154 T7552 T7535 punct ", ",mRNAs
R5155 T7553 T7535 advmod respectively,mRNAs
R5156 T7554 T7525 punct .,detects
R5157 T7556 T7557 det An,gel
R5158 T7557 T7565 nsubjpass gel,shown
R5159 T7558 T7559 compound ethidium,bromide
R5160 T7559 T7560 npadvmod bromide,stained
R5161 T7560 T7557 amod stained,gel
R5162 T7561 T7559 punct (,bromide
R5163 T7562 T7559 appos EtBr,bromide
R5164 T7563 T7560 punct ),stained
R5165 T7564 T7560 punct –,stained
R5166 T7566 T7557 acl showing,gel
R5167 T7567 T7568 det the,amount
R5168 T7568 T7566 dobj amount,showing
R5169 T7569 T7568 prep of,amount
R5170 T7570 T7571 amod total,RNA
R5171 T7571 T7569 pobj RNA,of
R5172 T7572 T7571 acl loaded,RNA
R5173 T7573 T7565 auxpass is,shown
R5174 T7574 T7565 advmod below,shown
R5175 T7575 T7565 punct .,shown