PMC:1584416 / 5817-24237 JSON TXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T1400	9-19	NN	denotes	Generation
T1401	20-22	IN	denotes	of
T1402	23-26	NN	denotes	Xpd
T1404	27-35	NN	denotes	Compound
T1403	36-49	NNS	denotes	Heterozygotes
T1405	49-202	sentence	denotes	We generated an Xpd knock-in allele with a point mutation encoding a single amino acid change (XPDG602D) found in the XPCS patient XPCS2 (Figure 1A–1C).
T1406	50-52	PRP	denotes	We
T1407	53-62	VBD	denotes	generated
T1408	63-65	DT	denotes	an
T1410	66-69	NN	denotes	Xpd
T1411	70-75	VB	denotes	knock
T1412	75-76	HYPH	denotes	-
T1413	76-78	RP	denotes	in
T1409	79-85	NN	denotes	allele
T1414	86-90	IN	denotes	with
T1415	91-92	DT	denotes	a
T1417	93-98	NN	denotes	point
T1416	99-107	NN	denotes	mutation
T1418	108-116	VBG	denotes	encoding
T1419	117-118	DT	denotes	a
T1421	119-125	JJ	denotes	single
T1422	126-131	NN	denotes	amino
T1423	132-136	NN	denotes	acid
T1420	137-143	NN	denotes	change
T1424	144-145	-LRB-	denotes	(
T1425	145-153	NN	denotes	XPDG602D
T1426	153-154	-RRB-	denotes	)
T1427	155-160	VBN	denotes	found
T1428	161-163	IN	denotes	in
T1429	164-167	DT	denotes	the
T1431	168-172	NN	denotes	XPCS
T1430	173-180	NN	denotes	patient
T1432	181-186	NN	denotes	XPCS2
T1433	187-188	-LRB-	denotes	(
T1435	188-194	NN	denotes	Figure
T1434	195-197	NN	denotes	1A
T1436	197-198	SYM	denotes	–
T1437	198-200	NN	denotes	1C
T1438	200-201	-RRB-	denotes	)
T1439	201-202	.	denotes	.
T1440	202-494	sentence	denotes	mRNA expression from the targeted allele could be detected in embryonic stem cells by RT-PCR (Figure 1D), although expression was reduced approximately 5-fold relative to wt mRNA transcript levels as determined by Northern blotting of RNA from the testis of heterozygous animals (Figure 1E).
T1441	203-207	NN	denotes	mRNA
T1442	208-218	NN	denotes	expression
T1444	219-223	IN	denotes	from
T1445	224-227	DT	denotes	the
T1447	228-236	VBN	denotes	targeted
T1446	237-243	NN	denotes	allele
T1448	244-249	MD	denotes	could
T1449	250-252	VB	denotes	be
T1443	253-261	VBN	denotes	detected
T1450	262-264	IN	denotes	in
T1451	265-274	JJ	denotes	embryonic
T1453	275-279	NN	denotes	stem
T1452	280-285	NNS	denotes	cells
T1454	286-288	IN	denotes	by
T1455	289-291	NN	denotes	RT
T1457	291-292	HYPH	denotes	-
T1456	292-295	NN	denotes	PCR
T1458	296-297	-LRB-	denotes	(
T1460	297-303	NN	denotes	Figure
T1459	304-306	NN	denotes	1D
T1461	306-307	-RRB-	denotes	)
T1462	307-309	,	denotes	,
T1463	309-317	IN	denotes	although
T1465	318-328	NN	denotes	expression
T1466	329-332	VBD	denotes	was
T1464	333-340	VBN	denotes	reduced
T1467	341-354	RB	denotes	approximately
T1468	355-356	CD	denotes	5
T1469	356-357	HYPH	denotes	-
T1470	357-361	RB	denotes	fold
T1471	362-370	JJ	denotes	relative
T1472	371-373	IN	denotes	to
T1473	374-376	NN	denotes	wt
T1475	377-381	NN	denotes	mRNA
T1476	382-392	NN	denotes	transcript
T1474	393-399	NNS	denotes	levels
T1477	400-402	IN	denotes	as
T1478	403-413	VBN	denotes	determined
T1479	414-416	IN	denotes	by
T1480	417-425	NNP	denotes	Northern
T1481	426-434	VBG	denotes	blotting
T1482	435-437	IN	denotes	of
T1483	438-441	NN	denotes	RNA
T1484	442-446	IN	denotes	from
T1485	447-450	DT	denotes	the
T1486	451-457	NN	denotes	testis
T1487	458-460	IN	denotes	of
T1488	461-473	JJ	denotes	heterozygous
T1489	474-481	NNS	denotes	animals
T1490	482-483	-LRB-	denotes	(
T1492	483-489	NN	denotes	Figure
T1491	490-492	NN	denotes	1E
T1493	492-493	-RRB-	denotes	)
T1494	493-494	.	denotes	.
T1495	494-678	sentence	denotes	Because patient XPCS2 was a hemizygote with mutant XPD protein (XPDG602D) expressed from a single allele, the corresponding mutation was expected to be viable in the homozygous state.
T1496	495-502	IN	denotes	Because
T1498	503-510	NN	denotes	patient
T1499	511-516	NN	denotes	XPCS2
T1497	517-520	VBD	denotes	was
T1501	521-522	DT	denotes	a
T1502	523-533	NN	denotes	hemizygote
T1503	534-538	IN	denotes	with
T1504	539-545	JJ	denotes	mutant
T1506	546-549	NN	denotes	XPD
T1505	550-557	NN	denotes	protein
T1507	558-559	-LRB-	denotes	(
T1508	559-567	NN	denotes	XPDG602D
T1509	567-568	-RRB-	denotes	)
T1510	569-578	VBN	denotes	expressed
T1511	579-583	IN	denotes	from
T1512	584-585	DT	denotes	a
T1514	586-592	JJ	denotes	single
T1513	593-599	NN	denotes	allele
T1515	599-601	,	denotes	,
T1516	601-604	DT	denotes	the
T1518	605-618	VBG	denotes	corresponding
T1517	619-627	NN	denotes	mutation
T1519	628-631	VBD	denotes	was
T1500	632-640	VBN	denotes	expected
T1520	641-643	TO	denotes	to
T1521	644-646	VB	denotes	be
T1522	647-653	JJ	denotes	viable
T1523	654-656	IN	denotes	in
T1524	657-660	DT	denotes	the
T1526	661-671	JJ	denotes	homozygous
T1525	672-677	NN	denotes	state
T1527	677-678	.	denotes	.
T1528	678-815	sentence	denotes	However, homozygous mutant mice were not observed, neither amongst live births nor embryonic day 13.5 (E13.5) or E3.5 embryos (Table 1).
T1529	679-686	RB	denotes	However
T1531	686-688	,	denotes	,
T1532	688-698	JJ	denotes	homozygous
T1534	699-705	JJ	denotes	mutant
T1533	706-710	NNS	denotes	mice
T1535	711-715	VBD	denotes	were
T1536	716-719	RB	denotes	not
T1530	720-728	VBN	denotes	observed
T1537	728-730	,	denotes	,
T1538	730-737	CC	denotes	neither
T1539	738-745	IN	denotes	amongst
T1540	746-750	JJ	denotes	live
T1541	751-757	NNS	denotes	births
T1542	758-761	CC	denotes	nor
T1543	762-771	JJ	denotes	embryonic
T1544	772-775	NN	denotes	day
T1546	776-780	CD	denotes	13.5
T1547	781-782	-LRB-	denotes	(
T1548	782-787	NN	denotes	E13.5
T1549	787-788	-RRB-	denotes	)
T1550	789-791	CC	denotes	or
T1551	792-796	NN	denotes	E3.5
T1545	797-804	NNS	denotes	embryos
T1552	805-806	-LRB-	denotes	(
T1553	806-811	NN	denotes	Table
T1554	812-813	CD	denotes	1
T1555	813-814	-RRB-	denotes	)
T1556	814-815	.	denotes	.
T1557	815-910	sentence	denotes	The corresponding hypomorphic, mutant allele was thus designated as homozygous lethal (†XPCS).
T1558	816-819	DT	denotes	The
T1560	820-833	VBG	denotes	corresponding
T1561	834-845	JJ	denotes	hypomorphic
T1562	845-847	,	denotes	,
T1563	847-853	JJ	denotes	mutant
T1559	854-860	NN	denotes	allele
T1565	861-864	VBD	denotes	was
T1566	865-869	RB	denotes	thus
T1564	870-880	VBN	denotes	designated
T1567	881-883	IN	denotes	as
T1568	884-894	JJ	denotes	homozygous
T1569	895-901	JJ	denotes	lethal
T1570	902-903	-LRB-	denotes	(
T1571	903-908	NN	denotes	†XPCS
T1572	908-909	-RRB-	denotes	)
T1573	909-910	.	denotes	.
T1574	910-1100	sentence	denotes	Homozygous lethality of the XPCS allele is likely due to reduced levels of expression of this essential protein as a result of gene targeting (Figure 1A) rather than to the mutation itself.
T1575	911-921	JJ	denotes	Homozygous
T1576	922-931	NN	denotes	lethality
T1578	932-934	IN	denotes	of
T1579	935-938	DT	denotes	the
T1581	939-943	NN	denotes	XPCS
T1580	944-950	NN	denotes	allele
T1577	951-953	VBZ	denotes	is
T1582	954-960	RB	denotes	likely
T1583	961-964	IN	denotes	due
T1584	965-967	IN	denotes	to
T1585	968-975	VBN	denotes	reduced
T1586	976-982	NNS	denotes	levels
T1587	983-985	IN	denotes	of
T1588	986-996	NN	denotes	expression
T1589	997-999	IN	denotes	of
T1590	1000-1004	DT	denotes	this
T1592	1005-1014	JJ	denotes	essential
T1591	1015-1022	NN	denotes	protein
T1593	1023-1025	IN	denotes	as
T1594	1026-1027	DT	denotes	a
T1595	1028-1034	NN	denotes	result
T1596	1035-1037	IN	denotes	of
T1597	1038-1042	NN	denotes	gene
T1598	1043-1052	NN	denotes	targeting
T1599	1053-1054	-LRB-	denotes	(
T1601	1054-1060	NN	denotes	Figure
T1600	1061-1063	NN	denotes	1A
T1602	1063-1064	-RRB-	denotes	)
T1603	1065-1071	JJ	denotes	rather
T1604	1072-1076	IN	denotes	than
T1605	1077-1079	IN	denotes	to
T1606	1080-1083	DT	denotes	the
T1607	1084-1092	NN	denotes	mutation
T1608	1093-1099	PRP	denotes	itself
T1609	1099-1100	.	denotes	.
T1610	1100-1212	sentence	denotes	Xpd ablation (XpdKO /KO ) is similarly incompatible with life beyond the earliest stages of embryogenesis [22].
T1611	1101-1104	NN	denotes	Xpd
T1612	1105-1113	NN	denotes	ablation
T1614	1114-1115	-LRB-	denotes	(
T1616	1115-1120	NN	denotes	XpdKO
T1617	1121-1122	HYPH	denotes	/
T1615	1122-1124	NN	denotes	KO
T1618	1125-1126	-RRB-	denotes	)
T1613	1127-1129	VBZ	denotes	is
T1619	1130-1139	RB	denotes	similarly
T1620	1140-1152	JJ	denotes	incompatible
T1621	1153-1157	IN	denotes	with
T1622	1158-1162	NN	denotes	life
T1623	1163-1169	IN	denotes	beyond
T1624	1170-1173	DT	denotes	the
T1626	1174-1182	JJS	denotes	earliest
T1625	1183-1189	NNS	denotes	stages
T1627	1190-1192	IN	denotes	of
T1628	1193-1206	NN	denotes	embryogenesis
T1629	1207-1208	-LRB-	denotes	[
T1630	1208-1210	CD	denotes	22
T1631	1210-1211	-RRB-	denotes	]
T1632	1211-1212	.	denotes	.
T1633	1212-1499	sentence	denotes	Consistent with this interpretation, a different targeted Xpd mutation encoding XPDR683W, which is associated with XP in the homozygous state in humans, was similarly underexpressed and lethal in the homozygous state (designated as †XP allele) (Figure 1A–1C; Table 1; unpublished data).
T1634	1213-1223	JJ	denotes	Consistent
T1636	1224-1228	IN	denotes	with
T1637	1229-1233	DT	denotes	this
T1638	1234-1248	NN	denotes	interpretation
T1639	1248-1250	,	denotes	,
T1640	1250-1251	DT	denotes	a
T1642	1252-1261	JJ	denotes	different
T1643	1262-1270	VBN	denotes	targeted
T1644	1271-1274	NN	denotes	Xpd
T1641	1275-1283	NN	denotes	mutation
T1645	1284-1292	VBG	denotes	encoding
T1646	1293-1301	NN	denotes	XPDR683W
T1647	1301-1303	,	denotes	,
T1648	1303-1308	WDT	denotes	which
T1650	1309-1311	VBZ	denotes	is
T1649	1312-1322	VBN	denotes	associated
T1651	1323-1327	IN	denotes	with
T1652	1328-1330	NN	denotes	XP
T1653	1331-1333	IN	denotes	in
T1654	1334-1337	DT	denotes	the
T1656	1338-1348	JJ	denotes	homozygous
T1655	1349-1354	NN	denotes	state
T1657	1355-1357	IN	denotes	in
T1658	1358-1364	NNS	denotes	humans
T1659	1364-1366	,	denotes	,
T1635	1366-1369	VBD	denotes	was
T1660	1370-1379	RB	denotes	similarly
T1661	1380-1394	JJ	denotes	underexpressed
T1662	1395-1398	CC	denotes	and
T1663	1399-1405	JJ	denotes	lethal
T1664	1406-1408	IN	denotes	in
T1665	1409-1412	DT	denotes	the
T1667	1413-1423	JJ	denotes	homozygous
T1666	1424-1429	NN	denotes	state
T1668	1430-1431	-LRB-	denotes	(
T1669	1431-1441	VBN	denotes	designated
T1670	1442-1444	IN	denotes	as
T1671	1445-1448	NN	denotes	†XP
T1672	1449-1455	NN	denotes	allele
T1673	1455-1456	-RRB-	denotes	)
T1674	1457-1458	-LRB-	denotes	(
T1676	1458-1464	NN	denotes	Figure
T1675	1465-1467	NN	denotes	1A
T1677	1467-1468	SYM	denotes	–
T1678	1468-1470	NN	denotes	1C
T1679	1470-1471	:	denotes	;
T1680	1472-1477	NN	denotes	Table
T1681	1478-1479	CD	denotes	1
T1682	1479-1480	:	denotes	;
T1683	1481-1492	JJ	denotes	unpublished
T1684	1493-1497	NNS	denotes	data
T1685	1497-1498	-RRB-	denotes	)
T1686	1498-1499	.	denotes	.
T1687	1499-1718	sentence	denotes	Also, a different targeting approach leading to the use of the native 3′UTR and removal of the neo gene resulted in normalisation of XpdXPCS mRNA levels and viable homozygous XpdXPCS/XPCS (XPDG602D/G602D) animals [23].
T1688	1500-1504	RB	denotes	Also
T1690	1504-1506	,	denotes	,
T1691	1506-1507	DT	denotes	a
T1693	1508-1517	JJ	denotes	different
T1694	1518-1527	NN	denotes	targeting
T1692	1528-1536	NN	denotes	approach
T1695	1537-1544	VBG	denotes	leading
T1696	1545-1547	IN	denotes	to
T1697	1548-1551	DT	denotes	the
T1698	1552-1555	NN	denotes	use
T1699	1556-1558	IN	denotes	of
T1700	1559-1562	DT	denotes	the
T1702	1563-1569	JJ	denotes	native
T1703	1570-1571	CD	denotes	3
T1704	1571-1572	SYM	denotes	′
T1701	1572-1575	NN	denotes	UTR
T1705	1576-1579	CC	denotes	and
T1706	1580-1587	NN	denotes	removal
T1707	1588-1590	IN	denotes	of
T1708	1591-1594	DT	denotes	the
T1710	1595-1598	NN	denotes	neo
T1709	1599-1603	NN	denotes	gene
T1689	1604-1612	VBD	denotes	resulted
T1711	1613-1615	IN	denotes	in
T1712	1616-1629	NN	denotes	normalisation
T1713	1630-1632	IN	denotes	of
T1714	1633-1640	NN	denotes	XpdXPCS
T1716	1641-1645	NN	denotes	mRNA
T1715	1646-1652	NNS	denotes	levels
T1717	1653-1656	CC	denotes	and
T1718	1657-1663	JJ	denotes	viable
T1720	1664-1674	JJ	denotes	homozygous
T1721	1675-1682	NN	denotes	XpdXPCS
T1723	1682-1683	HYPH	denotes	/
T1722	1683-1687	NN	denotes	XPCS
T1724	1688-1689	-LRB-	denotes	(
T1726	1689-1697	NN	denotes	XPDG602D
T1727	1697-1698	HYPH	denotes	/
T1725	1698-1703	NN	denotes	G602D
T1728	1703-1704	-RRB-	denotes	)
T1719	1705-1712	NNS	denotes	animals
T1729	1713-1714	-LRB-	denotes	[
T1730	1714-1716	CD	denotes	23
T1731	1716-1717	-RRB-	denotes	]
T1732	1717-1718	.	denotes	.
T7095	1729-1738	NN	denotes	Targeting
T7096	1739-1741	IN	denotes	of
T7097	1742-1745	DT	denotes	the
T7099	1746-1751	NN	denotes	Mouse
T7100	1752-1755	NN	denotes	Xpd
T7098	1756-1760	NN	denotes	Gene
T7101	1760-1881	sentence	denotes	(A) Schematic representation of the genomic structure and partial restriction map of the wt and targeted mouse Xpd loci.
T7102	1761-1762	-LRB-	denotes	(
T7103	1762-1763	LS	denotes	A
T7105	1763-1764	-RRB-	denotes	)
T7106	1765-1774	JJ	denotes	Schematic
T7104	1775-1789	NN	denotes	representation
T7107	1790-1792	IN	denotes	of
T7108	1793-1796	DT	denotes	the
T7110	1797-1804	JJ	denotes	genomic
T7109	1805-1814	NN	denotes	structure
T7111	1815-1818	CC	denotes	and
T7112	1819-1826	JJ	denotes	partial
T7114	1827-1838	NN	denotes	restriction
T7113	1839-1842	NN	denotes	map
T7115	1843-1845	IN	denotes	of
T7116	1846-1849	DT	denotes	the
T7118	1850-1852	NN	denotes	wt
T7119	1853-1856	CC	denotes	and
T7120	1857-1865	VBN	denotes	targeted
T7121	1866-1871	NN	denotes	mouse
T7122	1872-1875	NN	denotes	Xpd
T7117	1876-1880	NNS	denotes	loci
T7123	1880-1881	.	denotes	.
T7124	1881-2006	sentence	denotes	For the wt Xpd allele, shaded boxes represent coding regions of exons 12 and 19–23; the 3′UTR is represented by an open box.
T7125	1882-1885	IN	denotes	For
T7127	1886-1889	DT	denotes	the
T7129	1890-1892	NN	denotes	wt
T7130	1893-1896	NN	denotes	Xpd
T7128	1897-1903	NN	denotes	allele
T7131	1903-1905	,	denotes	,
T7132	1905-1911	JJ	denotes	shaded
T7133	1912-1917	NNS	denotes	boxes
T7134	1918-1927	VBP	denotes	represent
T7135	1928-1934	VBG	denotes	coding
T7136	1935-1942	NNS	denotes	regions
T7137	1943-1945	IN	denotes	of
T7138	1946-1951	NNS	denotes	exons
T7139	1952-1954	CD	denotes	12
T7140	1955-1958	CC	denotes	and
T7141	1959-1961	CD	denotes	19
T7142	1961-1962	SYM	denotes	–
T7143	1962-1964	CD	denotes	23
T7144	1964-1965	:	denotes	;
T7145	1966-1969	DT	denotes	the
T7147	1970-1971	CD	denotes	3
T7148	1971-1972	SYM	denotes	′
T7146	1972-1975	NN	denotes	UTR
T7149	1976-1978	VBZ	denotes	is
T7126	1979-1990	VBN	denotes	represented
T7150	1991-1993	IN	denotes	by
T7151	1994-1996	DT	denotes	an
T7153	1997-2001	JJ	denotes	open
T7152	2002-2005	NN	denotes	box
T7154	2005-2006	.	denotes	.
T7155	2006-2094	sentence	denotes	TGA indicates the translational stop codon; PolyA indicates the polyadenylation signal.
T7156	2007-2010	NN	denotes	TGA
T7157	2011-2020	VBZ	denotes	indicates
T7159	2021-2024	DT	denotes	the
T7161	2025-2038	JJ	denotes	translational
T7162	2039-2043	NN	denotes	stop
T7160	2044-2049	NN	denotes	codon
T7163	2049-2050	:	denotes	;
T7164	2051-2056	NN	denotes	PolyA
T7158	2057-2066	VBZ	denotes	indicates
T7165	2067-2070	DT	denotes	the
T7167	2071-2086	NN	denotes	polyadenylation
T7166	2087-2093	NN	denotes	signal
T7168	2093-2094	.	denotes	.
T7169	2094-2286	sentence	denotes	For the XpdTTD targeted allele, the 194–base pair (bp) human XPD cDNA fragment fused to exon 22 is indicated as a striped box including the TTD (R722W) mutation indicated by a vertical arrow.
T7170	2095-2098	IN	denotes	For
T7172	2099-2102	DT	denotes	the
T7174	2103-2109	NN	denotes	XpdTTD
T7175	2110-2118	VBN	denotes	targeted
T7173	2119-2125	NN	denotes	allele
T7176	2125-2127	,	denotes	,
T7177	2127-2130	DT	denotes	the
T7179	2131-2134	CD	denotes	194
T7181	2134-2135	HYPH	denotes	–
T7182	2135-2139	NN	denotes	base
T7180	2140-2144	NN	denotes	pair
T7183	2145-2146	-LRB-	denotes	(
T7184	2146-2148	NN	denotes	bp
T7185	2148-2149	-RRB-	denotes	)
T7186	2150-2155	JJ	denotes	human
T7187	2156-2159	NN	denotes	XPD
T7188	2160-2164	NN	denotes	cDNA
T7178	2165-2173	NN	denotes	fragment
T7189	2174-2179	VBN	denotes	fused
T7190	2180-2182	IN	denotes	to
T7191	2183-2187	NN	denotes	exon
T7192	2188-2190	CD	denotes	22
T7193	2191-2193	VBZ	denotes	is
T7171	2194-2203	VBN	denotes	indicated
T7194	2204-2206	IN	denotes	as
T7195	2207-2208	DT	denotes	a
T7197	2209-2216	VBN	denotes	striped
T7196	2217-2220	NN	denotes	box
T7198	2221-2230	VBG	denotes	including
T7199	2231-2234	DT	denotes	the
T7201	2235-2238	NN	denotes	TTD
T7202	2239-2240	-LRB-	denotes	(
T7203	2240-2245	NN	denotes	R722W
T7204	2245-2246	-RRB-	denotes	)
T7200	2247-2255	NN	denotes	mutation
T7205	2256-2265	VBN	denotes	indicated
T7206	2266-2268	IN	denotes	by
T7207	2269-2270	DT	denotes	a
T7209	2271-2279	JJ	denotes	vertical
T7208	2280-2285	NN	denotes	arrow
T7210	2285-2286	.	denotes	.
T7211	2286-2458	sentence	denotes	Chicken β-globin exons 2 and 3 including the 3′UTR are indicated as black boxes with corresponding Roman numerals followed by the β-globin polyadenylation signal (PolyA*).
T7212	2287-2294	NN	denotes	Chicken
T7214	2295-2296	NN	denotes	β
T7216	2296-2297	HYPH	denotes	-
T7215	2297-2303	NN	denotes	globin
T7217	2304-2309	NNS	denotes	exons
T7213	2310-2311	CD	denotes	2
T7219	2312-2315	CC	denotes	and
T7220	2316-2317	CD	denotes	3
T7221	2318-2327	VBG	denotes	including
T7222	2328-2331	DT	denotes	the
T7224	2332-2333	CD	denotes	3
T7225	2333-2334	HYPH	denotes	′
T7223	2334-2337	NN	denotes	UTR
T7226	2338-2341	VBP	denotes	are
T7218	2342-2351	VBN	denotes	indicated
T7227	2352-2354	IN	denotes	as
T7228	2355-2360	JJ	denotes	black
T7229	2361-2366	NNS	denotes	boxes
T7230	2367-2371	IN	denotes	with
T7231	2372-2385	VBG	denotes	corresponding
T7233	2386-2391	NNP	denotes	Roman
T7232	2392-2400	NNS	denotes	numerals
T7234	2401-2409	VBN	denotes	followed
T7235	2410-2412	IN	denotes	by
T7236	2413-2416	DT	denotes	the
T7238	2417-2418	NN	denotes	β
T7240	2418-2419	HYPH	denotes	-
T7239	2419-2425	NN	denotes	globin
T7241	2426-2441	NN	denotes	polyadenylation
T7237	2442-2448	NN	denotes	signal
T7242	2449-2450	-LRB-	denotes	(
T7243	2450-2456	NN	denotes	PolyA*
T7244	2456-2457	-RRB-	denotes	)
T7245	2457-2458	.	denotes	.
T7246	2458-2619	sentence	denotes	For the Xpd†XP and Xpd†XPCS targeted alleles, vertical arrows indicate XPCS (G602D-encoding) and XP (R683W-encoding) mutations in exons 19 and 22, respectively.
T7247	2459-2462	IN	denotes	For
T7249	2463-2466	DT	denotes	the
T7251	2467-2473	NN	denotes	Xpd†XP
T7252	2474-2477	CC	denotes	and
T7253	2478-2486	NN	denotes	Xpd†XPCS
T7254	2487-2495	VBN	denotes	targeted
T7250	2496-2503	NNS	denotes	alleles
T7255	2503-2505	,	denotes	,
T7256	2505-2513	JJ	denotes	vertical
T7257	2514-2520	NNS	denotes	arrows
T7248	2521-2529	VBP	denotes	indicate
T7258	2530-2534	NN	denotes	XPCS
T7260	2535-2536	-LRB-	denotes	(
T7262	2536-2541	NN	denotes	G602D
T7263	2541-2542	HYPH	denotes	-
T7261	2542-2550	VBG	denotes	encoding
T7264	2550-2551	-RRB-	denotes	)
T7265	2552-2555	CC	denotes	and
T7266	2556-2558	NN	denotes	XP
T7267	2559-2560	-LRB-	denotes	(
T7269	2560-2565	NN	denotes	R683W
T7270	2565-2566	HYPH	denotes	-
T7268	2566-2574	VBG	denotes	encoding
T7271	2574-2575	-RRB-	denotes	)
T7259	2576-2585	NNS	denotes	mutations
T7272	2586-2588	IN	denotes	in
T7273	2589-2594	NNS	denotes	exons
T7274	2595-2597	CD	denotes	19
T7275	2598-2601	CC	denotes	and
T7276	2602-2604	CD	denotes	22
T7277	2604-2606	,	denotes	,
T7278	2606-2618	RB	denotes	respectively
T7279	2618-2619	.	denotes	.
T7280	2619-2712	sentence	denotes	The unique 3′ probe located outside the targeting construct is marked by a thick black line.
T7281	2620-2623	DT	denotes	The
T7283	2624-2630	JJ	denotes	unique
T7284	2631-2632	CD	denotes	3
T7285	2632-2633	SYM	denotes	′
T7282	2634-2639	NN	denotes	probe
T7287	2640-2647	VBN	denotes	located
T7288	2648-2655	IN	denotes	outside
T7289	2656-2659	DT	denotes	the
T7291	2660-2669	VBG	denotes	targeting
T7290	2670-2679	NN	denotes	construct
T7292	2680-2682	VBZ	denotes	is
T7286	2683-2689	VBN	denotes	marked
T7293	2690-2692	IN	denotes	by
T7294	2693-2694	DT	denotes	a
T7296	2695-2700	JJ	denotes	thick
T7297	2701-2706	JJ	denotes	black
T7295	2707-2711	NN	denotes	line
T7298	2711-2712	.	denotes	.
T7299	2712-2792	sentence	denotes	Restriction sites: B, BamHI; C, ClaI; E, EcoRI; H, HindIII; Hp, HpaI; Sf, SfiI.
T7300	2713-2724	NN	denotes	Restriction
T7301	2725-2730	NNS	denotes	sites
T7302	2730-2732	:	denotes	:
T7303	2732-2733	NN	denotes	B
T7304	2733-2735	,	denotes	,
T7305	2735-2740	NN	denotes	BamHI
T7306	2740-2741	:	denotes	;
T7307	2742-2743	NN	denotes	C
T7308	2743-2745	,	denotes	,
T7309	2745-2749	NN	denotes	ClaI
T7310	2749-2750	:	denotes	;
T7311	2751-2752	NN	denotes	E
T7312	2752-2754	,	denotes	,
T7313	2754-2759	NN	denotes	EcoRI
T7314	2759-2760	:	denotes	;
T7315	2761-2762	NN	denotes	H
T7316	2762-2764	,	denotes	,
T7317	2764-2771	NN	denotes	HindIII
T7318	2771-2772	:	denotes	;
T7319	2773-2775	NN	denotes	Hp
T7320	2775-2777	,	denotes	,
T7321	2777-2781	NN	denotes	HpaI
T7322	2781-2782	:	denotes	;
T7323	2783-2785	NN	denotes	Sf
T7324	2785-2787	,	denotes	,
T7325	2787-2791	NN	denotes	SfiI
T7326	2791-2792	.	denotes	.
T7327	2792-2970	sentence	denotes	(B) Southern blot analysis of EcoRI-digested genomic DNA from wt, Xpd†XPCS/wt, and Xpd†XP/wt recombinant embryonic stem cell clones hybridised with the 3′ probe depicted in (A).
T7328	2793-2794	-LRB-	denotes	(
T7329	2794-2795	LS	denotes	B
T7331	2795-2796	-RRB-	denotes	)
T7332	2797-2805	NNP	denotes	Southern
T7333	2806-2810	NN	denotes	blot
T7330	2811-2819	NN	denotes	analysis
T7334	2820-2822	IN	denotes	of
T7335	2823-2828	NN	denotes	EcoRI
T7337	2828-2829	HYPH	denotes	-
T7336	2829-2837	VBN	denotes	digested
T7339	2838-2845	JJ	denotes	genomic
T7338	2846-2849	NN	denotes	DNA
T7340	2850-2854	IN	denotes	from
T7341	2855-2857	NN	denotes	wt
T7343	2857-2859	,	denotes	,
T7344	2859-2867	NN	denotes	Xpd†XPCS
T7346	2867-2868	HYPH	denotes	/
T7345	2868-2870	NN	denotes	wt
T7347	2870-2872	,	denotes	,
T7348	2872-2875	CC	denotes	and
T7349	2876-2882	NN	denotes	Xpd†XP
T7351	2882-2883	HYPH	denotes	/
T7350	2883-2885	NN	denotes	wt
T7352	2886-2897	JJ	denotes	recombinant
T7353	2898-2907	JJ	denotes	embryonic
T7355	2908-2912	NN	denotes	stem
T7354	2913-2917	NN	denotes	cell
T7342	2918-2924	NNS	denotes	clones
T7356	2925-2935	VBN	denotes	hybridised
T7357	2936-2940	IN	denotes	with
T7358	2941-2944	DT	denotes	the
T7360	2945-2946	CD	denotes	3
T7361	2946-2947	SYM	denotes	′
T7359	2948-2953	NN	denotes	probe
T7362	2954-2962	VBN	denotes	depicted
T7363	2963-2965	IN	denotes	in
T7364	2966-2967	-LRB-	denotes	(
T7365	2967-2968	NN	denotes	A
T7366	2968-2969	-RRB-	denotes	)
T7367	2969-2970	.	denotes	.
T7368	2970-3096	sentence	denotes	The wt allele yields a 6.5-kilobase (kb) fragment, whereas both targeted Xpd†XP and Xpd†XPCS alleles yield a 5.1-kb fragment.
T7369	2971-2974	DT	denotes	The
T7371	2975-2977	NN	denotes	wt
T7370	2978-2984	NN	denotes	allele
T7372	2985-2991	VBZ	denotes	yields
T7373	2992-2993	DT	denotes	a
T7375	2994-2997	CD	denotes	6.5
T7377	2997-2998	HYPH	denotes	-
T7376	2998-3006	NN	denotes	kilobase
T7378	3007-3008	-LRB-	denotes	(
T7379	3008-3010	NN	denotes	kb
T7380	3010-3011	-RRB-	denotes	)
T7374	3012-3020	NN	denotes	fragment
T7381	3020-3022	,	denotes	,
T7382	3022-3029	IN	denotes	whereas
T7384	3030-3034	CC	denotes	both
T7386	3035-3043	VBN	denotes	targeted
T7387	3044-3050	NN	denotes	Xpd†XP
T7388	3051-3054	CC	denotes	and
T7389	3055-3063	NN	denotes	Xpd†XPCS
T7385	3064-3071	NNS	denotes	alleles
T7383	3072-3077	VBP	denotes	yield
T7390	3078-3079	DT	denotes	a
T7392	3080-3083	CD	denotes	5.1
T7394	3083-3084	HYPH	denotes	-
T7393	3084-3086	NN	denotes	kb
T7391	3087-3095	NN	denotes	fragment
T7395	3095-3096	.	denotes	.
T7396	3096-3247	sentence	denotes	(C) Genotyping of wt and targeted alleles by PCR using primers F2, R1, and mR as indicated in (A) yields fragments of 399 bp and 468 bp, respectively.
T7397	3097-3098	-LRB-	denotes	(
T7398	3098-3099	LS	denotes	C
T7400	3099-3100	-RRB-	denotes	)
T7399	3101-3111	NN	denotes	Genotyping
T7401	3112-3114	IN	denotes	of
T7402	3115-3117	NN	denotes	wt
T7404	3118-3121	CC	denotes	and
T7405	3122-3130	VBN	denotes	targeted
T7403	3131-3138	NNS	denotes	alleles
T7406	3139-3141	IN	denotes	by
T7407	3142-3145	NN	denotes	PCR
T7408	3146-3151	VBG	denotes	using
T7409	3152-3159	NNS	denotes	primers
T7410	3160-3162	NN	denotes	F2
T7411	3162-3164	,	denotes	,
T7412	3164-3166	NN	denotes	R1
T7413	3166-3168	,	denotes	,
T7414	3168-3171	CC	denotes	and
T7415	3172-3174	NN	denotes	mR
T7416	3175-3177	IN	denotes	as
T7417	3178-3187	VBN	denotes	indicated
T7418	3188-3190	IN	denotes	in
T7419	3191-3192	-LRB-	denotes	(
T7420	3192-3193	LS	denotes	A
T7422	3193-3194	-RRB-	denotes	)
T7423	3195-3201	VBZ	denotes	yields
T7421	3202-3211	NNS	denotes	fragments
T7424	3212-3214	IN	denotes	of
T7425	3215-3218	CD	denotes	399
T7426	3219-3221	NN	denotes	bp
T7427	3222-3225	CC	denotes	and
T7428	3226-3229	CD	denotes	468
T7429	3230-3232	NN	denotes	bp
T7430	3232-3234	,	denotes	,
T7431	3234-3246	RB	denotes	respectively
T7432	3246-3247	.	denotes	.
T7433	3247-3491	sentence	denotes	(D) RT-PCR detection of mRNA expression originating from the targeted †XP and †XPCS alleles in embryonic stem cell clones using primers F1 (hybridising outside the targeting construct) and mR as indicated in (A) results in a 1,416-bp fragment.
T7434	3248-3249	-LRB-	denotes	(
T7435	3249-3250	LS	denotes	D
T7437	3250-3251	-RRB-	denotes	)
T7438	3252-3254	NN	denotes	RT
T7440	3254-3255	HYPH	denotes	-
T7439	3255-3258	NN	denotes	PCR
T7441	3259-3268	NN	denotes	detection
T7442	3269-3271	IN	denotes	of
T7443	3272-3276	NN	denotes	mRNA
T7444	3277-3287	NN	denotes	expression
T7445	3288-3299	VBG	denotes	originating
T7446	3300-3304	IN	denotes	from
T7447	3305-3308	DT	denotes	the
T7449	3309-3317	VBN	denotes	targeted
T7450	3318-3321	NN	denotes	†XP
T7451	3322-3325	CC	denotes	and
T7452	3326-3331	NN	denotes	†XPCS
T7448	3332-3339	NNS	denotes	alleles
T7453	3340-3342	IN	denotes	in
T7454	3343-3352	JJ	denotes	embryonic
T7456	3353-3357	NN	denotes	stem
T7455	3358-3362	NN	denotes	cell
T7457	3363-3369	NNS	denotes	clones
T7458	3370-3375	VBG	denotes	using
T7459	3376-3383	NNS	denotes	primers
T7460	3384-3386	NN	denotes	F1
T7461	3387-3388	-LRB-	denotes	(
T7462	3388-3399	VBG	denotes	hybridising
T7463	3400-3407	IN	denotes	outside
T7464	3408-3411	DT	denotes	the
T7466	3412-3421	VBG	denotes	targeting
T7465	3422-3431	NN	denotes	construct
T7467	3431-3432	-RRB-	denotes	)
T7468	3433-3436	CC	denotes	and
T7469	3437-3439	NN	denotes	mR
T7470	3440-3442	IN	denotes	as
T7471	3443-3452	VBN	denotes	indicated
T7472	3453-3455	IN	denotes	in
T7473	3456-3457	-LRB-	denotes	(
T7474	3457-3458	NN	denotes	A
T7475	3458-3459	-RRB-	denotes	)
T7436	3460-3467	VBZ	denotes	results
T7476	3468-3470	IN	denotes	in
T7477	3471-3472	DT	denotes	a
T7479	3473-3478	CD	denotes	1,416
T7481	3478-3479	HYPH	denotes	-
T7480	3479-3481	NN	denotes	bp
T7478	3482-3490	NN	denotes	fragment
T7482	3490-3491	.	denotes	.
T7483	3491-3683	sentence	denotes	(E) Northern blot analysis of total RNA isolated from testis of homozygous wt and XpdTTD/TTD, heterozygous Xpd†XPCS/wt and XpdTTD/wt, and compound heterozygous Xpd†XPCS/TTD mice as indicated.
T7484	3492-3493	-LRB-	denotes	(
T7485	3493-3494	LS	denotes	E
T7487	3494-3495	-RRB-	denotes	)
T7488	3496-3504	NNP	denotes	Northern
T7489	3505-3509	NN	denotes	blot
T7486	3510-3518	NN	denotes	analysis
T7490	3519-3521	IN	denotes	of
T7491	3522-3527	JJ	denotes	total
T7492	3528-3531	NN	denotes	RNA
T7493	3532-3540	VBN	denotes	isolated
T7494	3541-3545	IN	denotes	from
T7495	3546-3552	NN	denotes	testis
T7496	3553-3555	IN	denotes	of
T7497	3556-3566	JJ	denotes	homozygous
T7498	3567-3569	NN	denotes	wt
T7500	3570-3573	CC	denotes	and
T7501	3574-3580	NN	denotes	XpdTTD
T7503	3580-3581	HYPH	denotes	/
T7502	3581-3584	NN	denotes	TTD
T7504	3584-3586	,	denotes	,
T7505	3586-3598	JJ	denotes	heterozygous
T7507	3599-3607	NN	denotes	Xpd†XPCS
T7508	3607-3608	HYPH	denotes	/
T7506	3608-3610	NN	denotes	wt
T7509	3611-3614	CC	denotes	and
T7510	3615-3621	NN	denotes	XpdTTD
T7512	3621-3622	HYPH	denotes	/
T7511	3622-3624	NN	denotes	wt
T7513	3624-3626	,	denotes	,
T7514	3626-3629	CC	denotes	and
T7515	3630-3638	JJ	denotes	compound
T7517	3639-3651	JJ	denotes	heterozygous
T7518	3652-3660	NN	denotes	Xpd†XPCS
T7519	3660-3661	HYPH	denotes	/
T7516	3661-3664	NN	denotes	TTD
T7499	3665-3669	NNS	denotes	mice
T7520	3670-3672	IN	denotes	as
T7521	3673-3682	VBN	denotes	indicated
T7522	3682-3683	.	denotes	.
T7523	3683-3821	sentence	denotes	Hybridisation with a 1.4-kb mouse Xpd cDNA probe detects mRNAs of 4, 3.3, and 2.7 kb from wt, Xpd†XPCS, and XpdTTD alleles, respectively.
T7524	3684-3697	NN	denotes	Hybridisation
T7526	3698-3702	IN	denotes	with
T7527	3703-3704	DT	denotes	a
T7529	3705-3708	CD	denotes	1.4
T7531	3708-3709	HYPH	denotes	-
T7530	3709-3711	NN	denotes	kb
T7532	3712-3717	NN	denotes	mouse
T7533	3718-3721	NN	denotes	Xpd
T7534	3722-3726	NN	denotes	cDNA
T7528	3727-3732	NN	denotes	probe
T7525	3733-3740	VBZ	denotes	detects
T7535	3741-3746	NNS	denotes	mRNAs
T7536	3747-3749	IN	denotes	of
T7537	3750-3751	CD	denotes	4
T7539	3751-3753	,	denotes	,
T7540	3753-3756	CD	denotes	3.3
T7541	3756-3758	,	denotes	,
T7542	3758-3761	CC	denotes	and
T7543	3762-3765	CD	denotes	2.7
T7538	3766-3768	NN	denotes	kb
T7544	3769-3773	IN	denotes	from
T7545	3774-3776	NN	denotes	wt
T7547	3776-3778	,	denotes	,
T7548	3778-3786	NN	denotes	Xpd†XPCS
T7549	3786-3788	,	denotes	,
T7550	3788-3791	CC	denotes	and
T7551	3792-3798	NN	denotes	XpdTTD
T7546	3799-3806	NNS	denotes	alleles
T7552	3806-3808	,	denotes	,
T7553	3808-3820	RB	denotes	respectively
T7554	3820-3821	.	denotes	.
T7555	3821-3915	sentence	denotes	An ethidium bromide (EtBr)–stained gel showing the amount of total RNA loaded is shown below.
T7556	3822-3824	DT	denotes	An
T7558	3825-3833	NN	denotes	ethidium
T7559	3834-3841	NN	denotes	bromide
T7561	3842-3843	-LRB-	denotes	(
T7562	3843-3847	NN	denotes	EtBr
T7563	3847-3848	-RRB-	denotes	)
T7564	3848-3849	HYPH	denotes	–
T7560	3849-3856	VBN	denotes	stained
T7557	3857-3860	NN	denotes	gel
T7566	3861-3868	VBG	denotes	showing
T7567	3869-3872	DT	denotes	the
T7568	3873-3879	NN	denotes	amount
T7569	3880-3882	IN	denotes	of
T7570	3883-3888	JJ	denotes	total
T7571	3889-3892	NN	denotes	RNA
T7572	3893-3899	VBN	denotes	loaded
T7573	3900-3902	VBZ	denotes	is
T7565	3903-3908	VBN	denotes	shown
T7574	3909-3914	RB	denotes	below
T7575	3914-3915	.	denotes	.
T9211	3925-3934	NN	denotes	Frequency
T9212	3935-3937	IN	denotes	of
T9213	3938-3944	NN	denotes	Xpd†XP
T9215	3944-3945	HYPH	denotes	/
T9214	3945-3948	NN	denotes	†XP
T9216	3948-3950	,	denotes	,
T9217	3950-3958	NN	denotes	Xpd†XPCS
T9219	3958-3959	HYPH	denotes	/
T9218	3959-3964	NN	denotes	†XPCS
T9220	3964-3966	,	denotes	,
T9221	3966-3969	CC	denotes	and
T9222	3970-3978	NN	denotes	Compound
T9224	3979-3991	JJ	denotes	Heterozygous
T9225	3992-3998	NN	denotes	Xpd†XP
T9226	3998-3999	HYPH	denotes	/
T9223	3999-4004	NN	denotes	†XPCS
T9227	4005-4012	NNS	denotes	Embryos
T9228	4013-4016	CC	denotes	and
T9229	4017-4026	NN	denotes	Offspring
T1954	4028-4029	``	denotes	“
T1956	4029-4033	JJ	denotes	Null
T1957	4033-4034	''	denotes	”
T1955	4035-4041	NN	denotes	Allele
T1959	4042-4045	MD	denotes	Can
T1958	4046-4055	VB	denotes	Alleviate
T1960	4056-4069	JJ	denotes	Developmental
T1961	4070-4075	NN	denotes	Delay
T1962	4075-4077	,	denotes	,
T1963	4077-4081	NN	denotes	Skin
T1965	4081-4083	,	denotes	,
T1966	4083-4086	CC	denotes	and
T1967	4087-4091	NN	denotes	Hair
T1964	4092-4100	NNS	denotes	Features
T1968	4101-4103	IN	denotes	of
T1969	4104-4107	NN	denotes	TTD
T1970	4107-4329	sentence	denotes	To test the potential of a homozygous lethal “null” allele to nevertheless contribute to organismal phenotype, we combined an Xpd†XPCS allele with a viable XpdTTD allele by crossing the corresponding heterozygous animals.
T1971	4108-4110	TO	denotes	To
T1972	4111-4115	VB	denotes	test
T1974	4116-4119	DT	denotes	the
T1975	4120-4129	NN	denotes	potential
T1976	4130-4132	IN	denotes	of
T1977	4133-4134	DT	denotes	a
T1979	4135-4145	JJ	denotes	homozygous
T1980	4146-4152	JJ	denotes	lethal
T1981	4153-4154	``	denotes	“
T1982	4154-4158	JJ	denotes	null
T1983	4158-4159	''	denotes	”
T1978	4160-4166	NN	denotes	allele
T1984	4167-4169	TO	denotes	to
T1986	4170-4182	RB	denotes	nevertheless
T1985	4183-4193	VB	denotes	contribute
T1987	4194-4196	IN	denotes	to
T1988	4197-4207	JJ	denotes	organismal
T1989	4208-4217	NN	denotes	phenotype
T1990	4217-4219	,	denotes	,
T1991	4219-4221	PRP	denotes	we
T1973	4222-4230	VBD	denotes	combined
T1992	4231-4233	DT	denotes	an
T1994	4234-4242	NN	denotes	Xpd†XPCS
T1993	4243-4249	NN	denotes	allele
T1995	4250-4254	IN	denotes	with
T1996	4255-4256	DT	denotes	a
T1998	4257-4263	JJ	denotes	viable
T1999	4264-4270	NN	denotes	XpdTTD
T1997	4271-4277	NN	denotes	allele
T2000	4278-4280	IN	denotes	by
T2001	4281-4289	VBG	denotes	crossing
T2002	4290-4293	DT	denotes	the
T2004	4294-4307	VBG	denotes	corresponding
T2005	4308-4320	JJ	denotes	heterozygous
T2003	4321-4328	NNS	denotes	animals
T2006	4328-4329	.	denotes	.
T2007	4329-4500	sentence	denotes	Similar to hemizygous TTD mice carrying one true Xpd knockout allele (XpdTTD/KO), compound heterozygous XpdTTD/†XPCS mice were born at the expected Mendelian frequencies.
T2008	4330-4337	JJ	denotes	Similar
T2010	4338-4340	IN	denotes	to
T2011	4341-4351	JJ	denotes	hemizygous
T2013	4352-4355	NN	denotes	TTD
T2012	4356-4360	NNS	denotes	mice
T2014	4361-4369	VBG	denotes	carrying
T2015	4370-4373	CD	denotes	one
T2017	4374-4378	JJ	denotes	true
T2018	4379-4382	NN	denotes	Xpd
T2019	4383-4391	NN	denotes	knockout
T2016	4392-4398	NN	denotes	allele
T2020	4399-4400	-LRB-	denotes	(
T2022	4400-4406	NN	denotes	XpdTTD
T2023	4406-4407	HYPH	denotes	/
T2021	4407-4409	NN	denotes	KO
T2024	4409-4410	-RRB-	denotes	)
T2025	4410-4412	,	denotes	,
T2026	4412-4420	NN	denotes	compound
T2028	4421-4433	JJ	denotes	heterozygous
T2029	4434-4440	NN	denotes	XpdTTD
T2031	4440-4441	HYPH	denotes	/
T2030	4441-4446	NN	denotes	†XPCS
T2027	4447-4451	NNS	denotes	mice
T2032	4452-4456	VBD	denotes	were
T2009	4457-4461	VBN	denotes	born
T2033	4462-4464	IN	denotes	at
T2034	4465-4468	DT	denotes	the
T2036	4469-4477	VBN	denotes	expected
T2037	4478-4487	JJ	denotes	Mendelian
T2035	4488-4499	NNS	denotes	frequencies
T2038	4499-4500	.	denotes	.
T2039	4500-4696	sentence	denotes	Expression from the Xpd†XPCS allele was also reduced in the testis of compound heterozygous animals, whereas expression from the XpdTTD allele was increased relative to wt by ~5-fold (Figure 1E).
T2040	4501-4511	NN	denotes	Expression
T2042	4512-4516	IN	denotes	from
T2043	4517-4520	DT	denotes	the
T2045	4521-4529	NN	denotes	Xpd†XPCS
T2044	4530-4536	NN	denotes	allele
T2046	4537-4540	VBD	denotes	was
T2047	4541-4545	RB	denotes	also
T2041	4546-4553	VBN	denotes	reduced
T2048	4554-4556	IN	denotes	in
T2049	4557-4560	DT	denotes	the
T2050	4561-4567	NN	denotes	testis
T2051	4568-4570	IN	denotes	of
T2052	4571-4579	NN	denotes	compound
T2054	4580-4592	JJ	denotes	heterozygous
T2053	4593-4600	NNS	denotes	animals
T2055	4600-4602	,	denotes	,
T2056	4602-4609	IN	denotes	whereas
T2058	4610-4620	NN	denotes	expression
T2059	4621-4625	IN	denotes	from
T2060	4626-4629	DT	denotes	the
T2062	4630-4636	NN	denotes	XpdTTD
T2061	4637-4643	NN	denotes	allele
T2063	4644-4647	VBD	denotes	was
T2057	4648-4657	VBN	denotes	increased
T2064	4658-4666	JJ	denotes	relative
T2065	4667-4669	IN	denotes	to
T2066	4670-4672	NN	denotes	wt
T2067	4673-4675	IN	denotes	by
T2068	4676-4677	SYM	denotes	~
T2069	4677-4683	RB	denotes	5-fold
T2070	4684-4685	-LRB-	denotes	(
T2072	4685-4691	NN	denotes	Figure
T2071	4692-4694	NN	denotes	1E
T2073	4694-4695	-RRB-	denotes	)
T2074	4695-4696	.	denotes	.
T2075	4696-4949	sentence	denotes	Because of a lack of available antibodies and the inability to distinguish amongst various mutant forms of XPD differing only by single amino acid substitutions, we were unable to ascertain the relative amount of XPD protein from the different alleles.
T2076	4697-4704	IN	denotes	Because
T2078	4705-4707	IN	denotes	of
T2079	4708-4709	DT	denotes	a
T2080	4710-4714	NN	denotes	lack
T2081	4715-4717	IN	denotes	of
T2082	4718-4727	JJ	denotes	available
T2083	4728-4738	NNS	denotes	antibodies
T2084	4739-4742	CC	denotes	and
T2085	4743-4746	DT	denotes	the
T2086	4747-4756	NN	denotes	inability
T2087	4757-4759	TO	denotes	to
T2088	4760-4771	VB	denotes	distinguish
T2089	4772-4779	IN	denotes	amongst
T2090	4780-4787	JJ	denotes	various
T2092	4788-4794	JJ	denotes	mutant
T2091	4795-4800	NNS	denotes	forms
T2093	4801-4803	IN	denotes	of
T2094	4804-4807	NN	denotes	XPD
T2095	4808-4817	VBG	denotes	differing
T2096	4818-4822	RB	denotes	only
T2097	4823-4825	IN	denotes	by
T2098	4826-4832	JJ	denotes	single
T2100	4833-4838	NN	denotes	amino
T2101	4839-4843	NN	denotes	acid
T2099	4844-4857	NNS	denotes	substitutions
T2102	4857-4859	,	denotes	,
T2103	4859-4861	PRP	denotes	we
T2077	4862-4866	VBD	denotes	were
T2104	4867-4873	JJ	denotes	unable
T2105	4874-4876	TO	denotes	to
T2106	4877-4886	VB	denotes	ascertain
T2107	4887-4890	DT	denotes	the
T2109	4891-4899	JJ	denotes	relative
T2108	4900-4906	NN	denotes	amount
T2110	4907-4909	IN	denotes	of
T2111	4910-4913	NN	denotes	XPD
T2112	4914-4921	NN	denotes	protein
T2113	4922-4926	IN	denotes	from
T2114	4927-4930	DT	denotes	the
T2116	4931-4940	JJ	denotes	different
T2115	4941-4948	NNS	denotes	alleles
T2117	4948-4949	.	denotes	.
T2118	4949-5256	sentence	denotes	Despite reduced levels of mRNA expression, the homozygous lethal Xpd†XPCS allele ameliorated multiple XpdTTD-associated disease symptoms in compound heterozygous XpdTTD/†XPCS animals including the hallmark brittle hair and cutaneous features fully penetrant in homo- and hemizygous TTD mice (Figure 2A–2C).
T2119	4950-4957	IN	denotes	Despite
T2121	4958-4965	VBN	denotes	reduced
T2122	4966-4972	NNS	denotes	levels
T2123	4973-4975	IN	denotes	of
T2124	4976-4980	NN	denotes	mRNA
T2125	4981-4991	NN	denotes	expression
T2126	4991-4993	,	denotes	,
T2127	4993-4996	DT	denotes	the
T2129	4997-5007	JJ	denotes	homozygous
T2130	5008-5014	JJ	denotes	lethal
T2131	5015-5023	NN	denotes	Xpd†XPCS
T2128	5024-5030	NN	denotes	allele
T2120	5031-5042	VBD	denotes	ameliorated
T2132	5043-5051	JJ	denotes	multiple
T2134	5052-5058	NN	denotes	XpdTTD
T2136	5058-5059	HYPH	denotes	-
T2135	5059-5069	JJ	denotes	associated
T2137	5070-5077	NN	denotes	disease
T2133	5078-5086	NNS	denotes	symptoms
T2138	5087-5089	IN	denotes	in
T2139	5090-5098	JJ	denotes	compound
T2141	5099-5111	JJ	denotes	heterozygous
T2142	5112-5118	NN	denotes	XpdTTD
T2144	5118-5119	HYPH	denotes	/
T2143	5119-5124	NN	denotes	†XPCS
T2140	5125-5132	NNS	denotes	animals
T2145	5133-5142	VBG	denotes	including
T2146	5143-5146	DT	denotes	the
T2148	5147-5155	NN	denotes	hallmark
T2149	5156-5163	JJ	denotes	brittle
T2147	5164-5168	NN	denotes	hair
T2150	5169-5172	CC	denotes	and
T2151	5173-5182	JJ	denotes	cutaneous
T2152	5183-5191	NNS	denotes	features
T2153	5192-5197	RB	denotes	fully
T2154	5198-5207	JJ	denotes	penetrant
T2155	5208-5210	IN	denotes	in
T2156	5211-5215	AFX	denotes	homo
T2158	5215-5216	HYPH	denotes	-
T2159	5217-5220	CC	denotes	and
T2157	5221-5231	JJ	denotes	hemizygous
T2161	5232-5235	NN	denotes	TTD
T2160	5236-5240	NNS	denotes	mice
T2162	5241-5242	-LRB-	denotes	(
T2164	5242-5248	NN	denotes	Figure
T2163	5249-5251	NN	denotes	2A
T2165	5251-5252	SYM	denotes	–
T2166	5252-5254	NN	denotes	2C
T2167	5254-5255	-RRB-	denotes	)
T2168	5255-5256	.	denotes	.
T2169	5256-5580	sentence	denotes	In marked contrast to XpdTTD/TTD (and XpdTTD/KO) mice, which display complete hair loss in the first hair cycle and partial hair loss in subsequent cycles throughout their lives [21], compound heterozygous XpdTTD/†XPCS mice displayed some hair loss only during the first hair cycle and only locally at the back (Figure 2A).
T2170	5257-5259	IN	denotes	In
T2172	5260-5266	JJ	denotes	marked
T2173	5267-5275	NN	denotes	contrast
T2174	5276-5278	IN	denotes	to
T2175	5279-5285	NN	denotes	XpdTTD
T2177	5285-5286	HYPH	denotes	/
T2176	5286-5289	NN	denotes	TTD
T2179	5290-5291	-LRB-	denotes	(
T2180	5291-5294	CC	denotes	and
T2181	5295-5301	NN	denotes	XpdTTD
T2183	5301-5302	HYPH	denotes	/
T2182	5302-5304	NN	denotes	KO
T2184	5304-5305	-RRB-	denotes	)
T2178	5306-5310	NNS	denotes	mice
T2185	5310-5312	,	denotes	,
T2186	5312-5317	WDT	denotes	which
T2187	5318-5325	VBP	denotes	display
T2188	5326-5334	JJ	denotes	complete
T2190	5335-5339	NN	denotes	hair
T2189	5340-5344	NN	denotes	loss
T2191	5345-5347	IN	denotes	in
T2192	5348-5351	DT	denotes	the
T2194	5352-5357	JJ	denotes	first
T2195	5358-5362	NN	denotes	hair
T2193	5363-5368	NN	denotes	cycle
T2196	5369-5372	CC	denotes	and
T2197	5373-5380	JJ	denotes	partial
T2199	5381-5385	NN	denotes	hair
T2198	5386-5390	NN	denotes	loss
T2200	5391-5393	IN	denotes	in
T2201	5394-5404	JJ	denotes	subsequent
T2202	5405-5411	NNS	denotes	cycles
T2203	5412-5422	IN	denotes	throughout
T2204	5423-5428	PRP$	denotes	their
T2205	5429-5434	NNS	denotes	lives
T2206	5435-5436	-LRB-	denotes	[
T2207	5436-5438	CD	denotes	21
T2208	5438-5439	-RRB-	denotes	]
T2209	5439-5441	,	denotes	,
T2210	5441-5449	NN	denotes	compound
T2212	5450-5462	JJ	denotes	heterozygous
T2213	5463-5475	NN	denotes	XpdTTD/†XPCS
T2211	5476-5480	NNS	denotes	mice
T2171	5481-5490	VBD	denotes	displayed
T2214	5491-5495	DT	denotes	some
T2216	5496-5500	NN	denotes	hair
T2215	5501-5505	NN	denotes	loss
T2217	5506-5510	RB	denotes	only
T2218	5511-5517	IN	denotes	during
T2219	5518-5521	DT	denotes	the
T2221	5522-5527	JJ	denotes	first
T2222	5528-5532	NN	denotes	hair
T2220	5533-5538	NN	denotes	cycle
T2223	5539-5542	CC	denotes	and
T2224	5543-5547	RB	denotes	only
T2225	5548-5555	RB	denotes	locally
T2226	5556-5558	IN	denotes	at
T2227	5559-5562	DT	denotes	the
T2228	5563-5567	NN	denotes	back
T2229	5568-5569	-LRB-	denotes	(
T2231	5569-5575	NN	denotes	Figure
T2230	5576-5578	NN	denotes	2A
T2232	5578-5579	-RRB-	denotes	)
T2233	5579-5580	.	denotes	.
T2234	5580-5774	sentence	denotes	Scanning electron microscope analysis of XpdTTD/†XPCS hair revealed an almost normal appearance, with TTD-like features such as broken hairs found only at very low frequency (unpublished data).
T2235	5581-5589	NN	denotes	Scanning
T2237	5590-5598	NN	denotes	electron
T2236	5599-5609	NN	denotes	microscope
T2238	5610-5618	NN	denotes	analysis
T2240	5619-5621	IN	denotes	of
T2241	5622-5628	NN	denotes	XpdTTD
T2243	5628-5629	HYPH	denotes	/
T2242	5629-5634	NN	denotes	†XPCS
T2244	5635-5639	NN	denotes	hair
T2239	5640-5648	VBD	denotes	revealed
T2245	5649-5651	DT	denotes	an
T2247	5652-5658	RB	denotes	almost
T2248	5659-5665	JJ	denotes	normal
T2246	5666-5676	NN	denotes	appearance
T2249	5676-5678	,	denotes	,
T2250	5678-5682	IN	denotes	with
T2251	5683-5686	NN	denotes	TTD
T2253	5686-5687	HYPH	denotes	-
T2252	5687-5691	JJ	denotes	like
T2254	5692-5700	NNS	denotes	features
T2255	5701-5705	JJ	denotes	such
T2256	5706-5708	IN	denotes	as
T2257	5709-5715	JJ	denotes	broken
T2258	5716-5721	NNS	denotes	hairs
T2259	5722-5727	VBN	denotes	found
T2260	5728-5732	RB	denotes	only
T2261	5733-5735	IN	denotes	at
T2262	5736-5740	RB	denotes	very
T2263	5741-5744	JJ	denotes	low
T2264	5745-5754	NN	denotes	frequency
T2265	5755-5756	-LRB-	denotes	(
T2267	5756-5767	JJ	denotes	unpublished
T2266	5768-5772	NNS	denotes	data
T2268	5772-5773	-RRB-	denotes	)
T2269	5773-5774	.	denotes	.
T2270	5774-5961	sentence	denotes	Amino acid analysis confirmed that cysteine levels in the hair of the XpdTTD/†XPCS mice were significantly higher than in XpdTTD/TTD animals, but remained below the wt level (Figure 2C).
T2271	5775-5780	NN	denotes	Amino
T2272	5781-5785	NN	denotes	acid
T2273	5786-5794	NN	denotes	analysis
T2274	5795-5804	VBD	denotes	confirmed
T2275	5805-5809	IN	denotes	that
T2277	5810-5818	NN	denotes	cysteine
T2278	5819-5825	NNS	denotes	levels
T2279	5826-5828	IN	denotes	in
T2280	5829-5832	DT	denotes	the
T2281	5833-5837	NN	denotes	hair
T2282	5838-5840	IN	denotes	of
T2283	5841-5844	DT	denotes	the
T2285	5845-5851	NN	denotes	XpdTTD
T2287	5851-5852	HYPH	denotes	/
T2286	5852-5857	NN	denotes	†XPCS
T2284	5858-5862	NNS	denotes	mice
T2276	5863-5867	VBD	denotes	were
T2288	5868-5881	RB	denotes	significantly
T2289	5882-5888	JJR	denotes	higher
T2290	5889-5893	IN	denotes	than
T2291	5894-5896	IN	denotes	in
T2292	5897-5903	NN	denotes	XpdTTD
T2294	5903-5904	HYPH	denotes	/
T2293	5904-5907	NN	denotes	TTD
T2295	5908-5915	NNS	denotes	animals
T2296	5915-5917	,	denotes	,
T2297	5917-5920	CC	denotes	but
T2298	5921-5929	VBD	denotes	remained
T2299	5930-5935	IN	denotes	below
T2300	5936-5939	DT	denotes	the
T2302	5940-5942	NN	denotes	wt
T2301	5943-5948	NN	denotes	level
T2303	5949-5950	-LRB-	denotes	(
T2305	5950-5956	NN	denotes	Figure
T2304	5957-5959	NN	denotes	2C
T2306	5959-5960	-RRB-	denotes	)
T2307	5960-5961	.	denotes	.
T2308	5961-6109	sentence	denotes	TTD hemizygotes (XpdTTD/KO) do not display significant differences in cutaneous features and longevity relative to homozygous XpdTTD/TTD mice [21].
T2309	5962-5965	NN	denotes	TTD
T2310	5966-5977	NNS	denotes	hemizygotes
T2312	5978-5979	-LRB-	denotes	(
T2314	5979-5985	NN	denotes	XpdTTD
T2315	5985-5986	HYPH	denotes	/
T2313	5986-5988	NN	denotes	KO
T2316	5988-5989	-RRB-	denotes	)
T2317	5990-5992	VBP	denotes	do
T2318	5993-5996	RB	denotes	not
T2311	5997-6004	VB	denotes	display
T2319	6005-6016	JJ	denotes	significant
T2320	6017-6028	NNS	denotes	differences
T2321	6029-6031	IN	denotes	in
T2322	6032-6041	JJ	denotes	cutaneous
T2323	6042-6050	NNS	denotes	features
T2324	6051-6054	CC	denotes	and
T2325	6055-6064	NN	denotes	longevity
T2326	6065-6073	JJ	denotes	relative
T2327	6074-6076	IN	denotes	to
T2328	6077-6087	JJ	denotes	homozygous
T2330	6088-6094	NN	denotes	XpdTTD
T2332	6094-6095	HYPH	denotes	/
T2331	6095-6098	NN	denotes	TTD
T2329	6099-6103	NNS	denotes	mice
T2333	6104-6105	-LRB-	denotes	[
T2334	6105-6107	CD	denotes	21
T2335	6107-6108	-RRB-	denotes	]
T2336	6108-6109	.	denotes	.
T2337	6109-7769	sentence	denotes	Figure 2 Partial Rescue of TTD Cutaneous, Blood, and Developmental Phenotypes in Compound Heterozygous XpdTTD/†XPCS Mice (A) Photographs of 5-mo-old homozygous XpdTTD/TTD, compound heterozygous XpdTTD/†XPCS, and wt mice. Insets: images of first-round hair loss. (B) Histological analysis of the skin of XpdTTD/TTD, XpdTTD/†XPCS, and wt mice. TTD-associated acanthosis (thicker epidermis, indicated by solid vertical line), pronounced granular layer (indicated by arrows), and sebacious gland hyperplasia (indicated by dotted vertical line) were absent in the epidermis of XpdTTD/†XPCS and wt mice. Magnification 400×. (C) Cysteine content of hair from wt, XpdTTD/TTD, and XpdTTD/†XPCS mice. The p-value indicates significant differences between mutants and wt, as well as between XpdTTD/TTD and XpdTTD/†XPCS mice. Error bars indicate standard error of the mean (SEM). (D) Hematocrit values from blood of XpdTTD/TTD and XpdTTD/†XPCS mice. The p-values indicate the significance of the difference relative to wt. Error bars indicate SEM. (E) Body weights of developing XpdTTD/TTD and XpdTTD/†XPCS mice after weaning plotted as a percentage of the weight of age-matched control wt and heterozygote (hz) littermates (set at 100%). Error bars indicate SEM. Other prominent TTD features in the epidermis, including acanthosis (thickening of the layer of the nucleated cells), hyperkeratosis (prominent thickening of the cornified layer), and pronounced granular layer and sebacious gland hyperplasia (causing greasy appearance of the hair), were absent in the skin of XpdTTD/†XPCS mice, as established by blind microscopic examination of skin sections (Figure 2B).
T7658	6120-6127	JJ	denotes	Partial
T7659	6128-6134	NN	denotes	Rescue
T7660	6135-6137	IN	denotes	of
T7661	6138-6141	NN	denotes	TTD
T7662	6142-6151	JJ	denotes	Cutaneous
T7663	6151-6153	,	denotes	,
T7664	6153-6158	NN	denotes	Blood
T7665	6158-6160	,	denotes	,
T7666	6160-6163	CC	denotes	and
T7667	6164-6177	JJ	denotes	Developmental
T7668	6178-6188	NNS	denotes	Phenotypes
T7669	6189-6191	IN	denotes	in
T7670	6192-6200	NN	denotes	Compound
T7672	6201-6213	JJ	denotes	Heterozygous
T7673	6214-6220	NN	denotes	XpdTTD
T7675	6220-6221	HYPH	denotes	/
T7674	6221-6226	NN	denotes	†XPCS
T7671	6227-6231	NNS	denotes	Mice
T7676	6231-6331	sentence	denotes	(A) Photographs of 5-mo-old homozygous XpdTTD/TTD, compound heterozygous XpdTTD/†XPCS, and wt mice.
T7677	6232-6233	-LRB-	denotes	(
T7678	6233-6234	LS	denotes	A
T7680	6234-6235	-RRB-	denotes	)
T7679	6236-6247	NNS	denotes	Photographs
T7681	6248-6250	IN	denotes	of
T7682	6251-6252	CD	denotes	5
T7684	6252-6253	HYPH	denotes	-
T7683	6253-6255	NN	denotes	mo
T7686	6255-6256	HYPH	denotes	-
T7685	6256-6259	JJ	denotes	old
T7688	6260-6270	JJ	denotes	homozygous
T7690	6271-6277	NN	denotes	XpdTTD
T7691	6277-6278	HYPH	denotes	/
T7689	6278-6281	NN	denotes	TTD
T7692	6281-6283	,	denotes	,
T7693	6283-6291	NN	denotes	compound
T7695	6292-6304	JJ	denotes	heterozygous
T7696	6305-6311	NN	denotes	XpdTTD
T7697	6311-6312	HYPH	denotes	/
T7694	6312-6317	NN	denotes	†XPCS
T7698	6317-6319	,	denotes	,
T7699	6319-6322	CC	denotes	and
T7700	6323-6325	NN	denotes	wt
T7687	6326-6330	NNS	denotes	mice
T7701	6330-6331	.	denotes	.
T7702	6331-6372	sentence	denotes	Insets: images of first-round hair loss.
T7703	6332-6338	NNS	denotes	Insets
T7704	6338-6340	:	denotes	:
T7705	6340-6346	NNS	denotes	images
T7706	6347-6349	IN	denotes	of
T7707	6350-6355	JJ	denotes	first
T7709	6355-6356	HYPH	denotes	-
T7708	6356-6361	NN	denotes	round
T7711	6362-6366	NN	denotes	hair
T7710	6367-6371	NN	denotes	loss
T7712	6371-6372	.	denotes	.
T7713	6372-6452	sentence	denotes	(B) Histological analysis of the skin of XpdTTD/TTD, XpdTTD/†XPCS, and wt mice.
T7714	6373-6374	-LRB-	denotes	(
T7715	6374-6375	LS	denotes	B
T7717	6375-6376	-RRB-	denotes	)
T7718	6377-6389	JJ	denotes	Histological
T7716	6390-6398	NN	denotes	analysis
T7719	6399-6401	IN	denotes	of
T7720	6402-6405	DT	denotes	the
T7721	6406-6410	NN	denotes	skin
T7722	6411-6413	IN	denotes	of
T7723	6414-6420	NN	denotes	XpdTTD
T7725	6420-6421	HYPH	denotes	/
T7724	6421-6424	NN	denotes	TTD
T7727	6424-6426	,	denotes	,
T7728	6426-6432	NN	denotes	XpdTTD
T7730	6432-6433	HYPH	denotes	/
T7729	6433-6438	NN	denotes	†XPCS
T7731	6438-6440	,	denotes	,
T7732	6440-6443	CC	denotes	and
T7733	6444-6446	NN	denotes	wt
T7726	6447-6451	NNS	denotes	mice
T7734	6451-6452	.	denotes	.
T7735	6452-6708	sentence	denotes	TTD-associated acanthosis (thicker epidermis, indicated by solid vertical line), pronounced granular layer (indicated by arrows), and sebacious gland hyperplasia (indicated by dotted vertical line) were absent in the epidermis of XpdTTD/†XPCS and wt mice.
T7736	6453-6456	NN	denotes	TTD
T7738	6456-6457	HYPH	denotes	-
T7737	6457-6467	JJ	denotes	associated
T7739	6468-6478	NN	denotes	acanthosis
T7741	6479-6480	-LRB-	denotes	(
T7743	6480-6487	JJR	denotes	thicker
T7742	6488-6497	NN	denotes	epidermis
T7744	6497-6499	,	denotes	,
T7745	6499-6508	VBN	denotes	indicated
T7746	6509-6511	IN	denotes	by
T7747	6512-6517	JJ	denotes	solid
T7749	6518-6526	JJ	denotes	vertical
T7748	6527-6531	NN	denotes	line
T7750	6531-6532	-RRB-	denotes	)
T7751	6532-6534	,	denotes	,
T7752	6534-6544	VBN	denotes	pronounced
T7754	6545-6553	JJ	denotes	granular
T7753	6554-6559	NN	denotes	layer
T7755	6560-6561	-LRB-	denotes	(
T7756	6561-6570	VBN	denotes	indicated
T7757	6571-6573	IN	denotes	by
T7758	6574-6580	NNS	denotes	arrows
T7759	6580-6581	-RRB-	denotes	)
T7760	6581-6583	,	denotes	,
T7761	6583-6586	CC	denotes	and
T7762	6587-6596	JJ	denotes	sebacious
T7764	6597-6602	NN	denotes	gland
T7763	6603-6614	NN	denotes	hyperplasia
T7765	6615-6616	-LRB-	denotes	(
T7766	6616-6625	VBN	denotes	indicated
T7767	6626-6628	IN	denotes	by
T7768	6629-6635	VBN	denotes	dotted
T7770	6636-6644	JJ	denotes	vertical
T7769	6645-6649	NN	denotes	line
T7771	6649-6650	-RRB-	denotes	)
T7740	6651-6655	VBD	denotes	were
T7772	6656-6662	JJ	denotes	absent
T7773	6663-6665	IN	denotes	in
T7774	6666-6669	DT	denotes	the
T7775	6670-6679	NN	denotes	epidermis
T7776	6680-6682	IN	denotes	of
T7777	6683-6689	NN	denotes	XpdTTD
T7779	6689-6690	HYPH	denotes	/
T7778	6690-6695	NN	denotes	†XPCS
T7781	6696-6699	CC	denotes	and
T7782	6700-6702	NN	denotes	wt
T7780	6703-6707	NNS	denotes	mice
T7783	6707-6708	.	denotes	.
T7784	6708-6728	sentence	denotes	Magnification 400×.
T7785	6709-6722	NN	denotes	Magnification
T7786	6723-6726	CD	denotes	400
T7787	6726-6727	SYM	denotes	×
T7788	6727-6728	.	denotes	.
T7789	6728-6801	sentence	denotes	(C) Cysteine content of hair from wt, XpdTTD/TTD, and XpdTTD/†XPCS mice.
T7790	6729-6730	-LRB-	denotes	(
T7791	6730-6731	LS	denotes	C
T7793	6731-6732	-RRB-	denotes	)
T7794	6733-6741	NN	denotes	Cysteine
T7792	6742-6749	NN	denotes	content
T7795	6750-6752	IN	denotes	of
T7796	6753-6757	NN	denotes	hair
T7797	6758-6762	IN	denotes	from
T7798	6763-6765	NN	denotes	wt
T7800	6765-6767	,	denotes	,
T7801	6767-6773	NN	denotes	XpdTTD
T7803	6773-6774	HYPH	denotes	/
T7802	6774-6777	NN	denotes	TTD
T7804	6777-6779	,	denotes	,
T7805	6779-6782	CC	denotes	and
T7806	6783-6789	NN	denotes	XpdTTD
T7808	6789-6790	HYPH	denotes	/
T7807	6790-6795	NN	denotes	†XPCS
T7799	6796-6800	NNS	denotes	mice
T7809	6800-6801	.	denotes	.
T7810	6801-6924	sentence	denotes	The p-value indicates significant differences between mutants and wt, as well as between XpdTTD/TTD and XpdTTD/†XPCS mice.
T7811	6802-6805	DT	denotes	The
T7813	6806-6807	NN	denotes	p
T7814	6807-6808	HYPH	denotes	-
T7812	6808-6813	NN	denotes	value
T7815	6814-6823	VBZ	denotes	indicates
T7816	6824-6835	JJ	denotes	significant
T7817	6836-6847	NNS	denotes	differences
T7818	6848-6855	IN	denotes	between
T7819	6856-6863	NNS	denotes	mutants
T7820	6864-6867	CC	denotes	and
T7821	6868-6870	NN	denotes	wt
T7822	6870-6872	,	denotes	,
T7823	6872-6874	RB	denotes	as
T7825	6875-6879	RB	denotes	well
T7824	6880-6882	IN	denotes	as
T7826	6883-6890	IN	denotes	between
T7827	6891-6897	NN	denotes	XpdTTD
T7829	6897-6898	HYPH	denotes	/
T7828	6898-6901	NN	denotes	TTD
T7831	6902-6905	CC	denotes	and
T7832	6906-6912	NN	denotes	XpdTTD
T7834	6912-6913	HYPH	denotes	/
T7833	6913-6918	NN	denotes	†XPCS
T7830	6919-6923	NNS	denotes	mice
T7835	6923-6924	.	denotes	.
T7836	6924-6978	sentence	denotes	Error bars indicate standard error of the mean (SEM).
T7837	6925-6930	NN	denotes	Error
T7838	6931-6935	NNS	denotes	bars
T7839	6936-6944	VBP	denotes	indicate
T7840	6945-6953	JJ	denotes	standard
T7841	6954-6959	NN	denotes	error
T7842	6960-6962	IN	denotes	of
T7843	6963-6966	DT	denotes	the
T7844	6967-6971	NN	denotes	mean
T7845	6972-6973	-LRB-	denotes	(
T7846	6973-6976	NN	denotes	SEM
T7847	6976-6977	-RRB-	denotes	)
T7848	6977-6978	.	denotes	.
T7849	6978-7048	sentence	denotes	(D) Hematocrit values from blood of XpdTTD/TTD and XpdTTD/†XPCS mice.
T7850	6979-6980	-LRB-	denotes	(
T7851	6980-6981	LS	denotes	D
T7853	6981-6982	-RRB-	denotes	)
T7854	6983-6993	NN	denotes	Hematocrit
T7852	6994-7000	NNS	denotes	values
T7855	7001-7005	IN	denotes	from
T7856	7006-7011	NN	denotes	blood
T7857	7012-7014	IN	denotes	of
T7858	7015-7021	NN	denotes	XpdTTD
T7860	7021-7022	HYPH	denotes	/
T7859	7022-7025	NN	denotes	TTD
T7862	7026-7029	CC	denotes	and
T7863	7030-7036	NN	denotes	XpdTTD
T7865	7036-7037	HYPH	denotes	/
T7864	7037-7042	NN	denotes	†XPCS
T7861	7043-7047	NNS	denotes	mice
T7866	7047-7048	.	denotes	.
T7867	7048-7121	sentence	denotes	The p-values indicate the significance of the difference relative to wt.
T7868	7049-7052	DT	denotes	The
T7870	7053-7054	NN	denotes	p
T7871	7054-7055	HYPH	denotes	-
T7869	7055-7061	NNS	denotes	values
T7872	7062-7070	VBP	denotes	indicate
T7873	7071-7074	DT	denotes	the
T7874	7075-7087	NN	denotes	significance
T7875	7088-7090	IN	denotes	of
T7876	7091-7094	DT	denotes	the
T7877	7095-7105	NN	denotes	difference
T7878	7106-7114	JJ	denotes	relative
T7879	7115-7117	IN	denotes	to
T7880	7118-7120	NN	denotes	wt
T7881	7120-7121	.	denotes	.
T7882	7121-7146	sentence	denotes	Error bars indicate SEM.
T7883	7122-7127	NN	denotes	Error
T7884	7128-7132	NNS	denotes	bars
T7885	7133-7141	VBP	denotes	indicate
T7886	7142-7145	NN	denotes	SEM
T7887	7145-7146	.	denotes	.
T7888	7146-7337	sentence	denotes	(E) Body weights of developing XpdTTD/TTD and XpdTTD/†XPCS mice after weaning plotted as a percentage of the weight of age-matched control wt and heterozygote (hz) littermates (set at 100%).
T7889	7147-7148	-LRB-	denotes	(
T7890	7148-7149	LS	denotes	E
T7892	7149-7150	-RRB-	denotes	)
T7893	7151-7155	NN	denotes	Body
T7891	7156-7163	NNS	denotes	weights
T7894	7164-7166	IN	denotes	of
T7895	7167-7177	VBG	denotes	developing
T7897	7178-7184	NN	denotes	XpdTTD
T7899	7184-7185	HYPH	denotes	/
T7898	7185-7188	NN	denotes	TTD
T7900	7189-7192	CC	denotes	and
T7901	7193-7199	NN	denotes	XpdTTD
T7903	7199-7200	HYPH	denotes	/
T7902	7200-7205	NN	denotes	†XPCS
T7896	7206-7210	NNS	denotes	mice
T7904	7211-7216	IN	denotes	after
T7905	7217-7224	VBG	denotes	weaning
T7906	7225-7232	VBN	denotes	plotted
T7907	7233-7235	IN	denotes	as
T7908	7236-7237	DT	denotes	a
T7909	7238-7248	NN	denotes	percentage
T7910	7249-7251	IN	denotes	of
T7911	7252-7255	DT	denotes	the
T7912	7256-7262	NN	denotes	weight
T7913	7263-7265	IN	denotes	of
T7914	7266-7269	NN	denotes	age
T7916	7269-7270	HYPH	denotes	-
T7915	7270-7277	JJ	denotes	matched
T7918	7278-7285	NN	denotes	control
T7919	7286-7288	NN	denotes	wt
T7920	7289-7292	CC	denotes	and
T7921	7293-7305	NN	denotes	heterozygote
T7922	7306-7307	-LRB-	denotes	(
T7923	7307-7309	NN	denotes	hz
T7924	7309-7310	-RRB-	denotes	)
T7917	7311-7322	NNS	denotes	littermates
T7925	7323-7324	-LRB-	denotes	(
T7926	7324-7327	VBN	denotes	set
T7927	7328-7330	IN	denotes	at
T7928	7331-7334	CD	denotes	100
T7929	7334-7335	NN	denotes	%
T7930	7335-7336	-RRB-	denotes	)
T7931	7336-7337	.	denotes	.
T7932	7337-7362	sentence	denotes	Error bars indicate SEM.
T7933	7338-7343	NN	denotes	Error
T7934	7344-7348	NNS	denotes	bars
T7935	7349-7357	VBP	denotes	indicate
T7936	7358-7361	NN	denotes	SEM
T7937	7361-7362	.	denotes	.
T2338	7363-7368	JJ	denotes	Other
T2340	7369-7378	JJ	denotes	prominent
T2341	7379-7382	NN	denotes	TTD
T2339	7383-7391	NNS	denotes	features
T2343	7392-7394	IN	denotes	in
T2344	7395-7398	DT	denotes	the
T2345	7399-7408	NN	denotes	epidermis
T2346	7408-7410	,	denotes	,
T2347	7410-7419	VBG	denotes	including
T2348	7420-7430	NN	denotes	acanthosis
T2349	7431-7432	-LRB-	denotes	(
T2350	7432-7442	VBG	denotes	thickening
T2351	7443-7445	IN	denotes	of
T2352	7446-7449	DT	denotes	the
T2353	7450-7455	NN	denotes	layer
T2354	7456-7458	IN	denotes	of
T2355	7459-7462	DT	denotes	the
T2357	7463-7472	JJ	denotes	nucleated
T2356	7473-7478	NNS	denotes	cells
T2358	7478-7479	-RRB-	denotes	)
T2359	7479-7481	,	denotes	,
T2360	7481-7495	NN	denotes	hyperkeratosis
T2361	7496-7497	-LRB-	denotes	(
T2363	7497-7506	JJ	denotes	prominent
T2362	7507-7517	NN	denotes	thickening
T2364	7518-7520	IN	denotes	of
T2365	7521-7524	DT	denotes	the
T2367	7525-7534	VBN	denotes	cornified
T2366	7535-7540	NN	denotes	layer
T2368	7540-7541	-RRB-	denotes	)
T2369	7541-7543	,	denotes	,
T2370	7543-7546	CC	denotes	and
T2371	7547-7557	JJ	denotes	pronounced
T2373	7558-7566	JJ	denotes	granular
T2372	7567-7572	NN	denotes	layer
T2374	7573-7576	CC	denotes	and
T2375	7577-7586	JJ	denotes	sebacious
T2376	7587-7592	NN	denotes	gland
T2377	7593-7604	NN	denotes	hyperplasia
T2378	7605-7606	-LRB-	denotes	(
T2379	7606-7613	VBG	denotes	causing
T2380	7614-7620	JJ	denotes	greasy
T2381	7621-7631	NN	denotes	appearance
T2382	7632-7634	IN	denotes	of
T2383	7635-7638	DT	denotes	the
T2384	7639-7643	NN	denotes	hair
T2385	7643-7644	-RRB-	denotes	)
T2386	7644-7646	,	denotes	,
T2342	7646-7650	VBD	denotes	were
T2387	7651-7657	JJ	denotes	absent
T2388	7658-7660	IN	denotes	in
T2389	7661-7664	DT	denotes	the
T2390	7665-7669	NN	denotes	skin
T2391	7670-7672	IN	denotes	of
T2392	7673-7679	NN	denotes	XpdTTD
T2394	7679-7680	HYPH	denotes	/
T2393	7680-7685	NN	denotes	†XPCS
T2395	7686-7690	NNS	denotes	mice
T2396	7690-7692	,	denotes	,
T2397	7692-7694	IN	denotes	as
T2398	7695-7706	VBN	denotes	established
T2399	7707-7709	IN	denotes	by
T2400	7710-7715	JJ	denotes	blind
T2402	7716-7727	JJ	denotes	microscopic
T2401	7728-7739	NN	denotes	examination
T2403	7740-7742	IN	denotes	of
T2404	7743-7747	NN	denotes	skin
T2405	7748-7756	NNS	denotes	sections
T2406	7757-7758	-LRB-	denotes	(
T2408	7758-7764	NN	denotes	Figure
T2407	7765-7767	NN	denotes	2B
T2409	7767-7768	-RRB-	denotes	)
T2410	7768-7769	.	denotes	.
T2411	7769-7967	sentence	denotes	Furthermore, anaemia and developmental delay present in patients with TTD [24] and in XpdTTD/TTD mice [15] were both partially rescued in compound heterozygous XpdTTD/†XPCS mice (Figure 2D and 2E).
T2412	7770-7781	RB	denotes	Furthermore
T2414	7781-7783	,	denotes	,
T2415	7783-7790	NN	denotes	anaemia
T2416	7791-7794	CC	denotes	and
T2417	7795-7808	JJ	denotes	developmental
T2418	7809-7814	NN	denotes	delay
T2419	7815-7822	JJ	denotes	present
T2420	7823-7825	IN	denotes	in
T2421	7826-7834	NNS	denotes	patients
T2422	7835-7839	IN	denotes	with
T2423	7840-7843	NN	denotes	TTD
T2424	7844-7845	-LRB-	denotes	[
T2425	7845-7847	CD	denotes	24
T2426	7847-7848	-RRB-	denotes	]
T2427	7849-7852	CC	denotes	and
T2428	7853-7855	IN	denotes	in
T2429	7856-7862	NN	denotes	XpdTTD
T2431	7862-7863	HYPH	denotes	/
T2430	7863-7866	NN	denotes	TTD
T2432	7867-7871	NNS	denotes	mice
T2433	7872-7873	-LRB-	denotes	[
T2434	7873-7875	CD	denotes	15
T2435	7875-7876	-RRB-	denotes	]
T2436	7877-7881	VBD	denotes	were
T2437	7882-7886	RB	denotes	both
T2438	7887-7896	RB	denotes	partially
T2413	7897-7904	VBN	denotes	rescued
T2439	7905-7907	IN	denotes	in
T2440	7908-7916	JJ	denotes	compound
T2442	7917-7929	JJ	denotes	heterozygous
T2443	7930-7936	NN	denotes	XpdTTD
T2445	7936-7937	HYPH	denotes	/
T2444	7937-7942	NN	denotes	†XPCS
T2441	7943-7947	NNS	denotes	mice
T2446	7948-7949	-LRB-	denotes	(
T2448	7949-7955	NN	denotes	Figure
T2447	7956-7958	NN	denotes	2D
T2449	7959-7962	CC	denotes	and
T2450	7963-7965	NN	denotes	2E
T2451	7965-7966	-RRB-	denotes	)
T2452	7966-7967	.	denotes	.
T2575	7989-7997	NNS	denotes	Features
T2576	7998-8000	IN	denotes	in
T2577	8001-8004	NN	denotes	TTD
T2578	8005-8009	NNS	denotes	Mice
T2579	8010-8012	IN	denotes	by
T2580	8013-8023	JJ	denotes	Homozygous
T2582	8024-8030	JJ	denotes	Lethal
T2583	8031-8034	NN	denotes	Xpd
T2581	8035-8042	NNS	denotes	Alleles
T2584	8042-8276	sentence	denotes	Because patients with TTD, XPCS, and CS (but not XP) and the corresponding mouse models share similar accelerated progeroid symptoms [12,13,15,23], we next addressed ageing-related parameters in compound heterozygous mice (Figure 3).
T2585	8043-8050	IN	denotes	Because
T2587	8051-8059	NNS	denotes	patients
T2588	8060-8064	IN	denotes	with
T2589	8065-8068	NN	denotes	TTD
T2590	8068-8070	,	denotes	,
T2591	8070-8074	NN	denotes	XPCS
T2592	8074-8076	,	denotes	,
T2593	8076-8079	CC	denotes	and
T2594	8080-8082	NN	denotes	CS
T2595	8083-8084	-LRB-	denotes	(
T2596	8084-8087	CC	denotes	but
T2597	8088-8091	RB	denotes	not
T2598	8092-8094	NN	denotes	XP
T2599	8094-8095	-RRB-	denotes	)
T2600	8096-8099	CC	denotes	and
T2601	8100-8103	DT	denotes	the
T2603	8104-8117	VBG	denotes	corresponding
T2604	8118-8123	NN	denotes	mouse
T2602	8124-8130	NNS	denotes	models
T2586	8131-8136	VBP	denotes	share
T2606	8137-8144	JJ	denotes	similar
T2608	8145-8156	VBN	denotes	accelerated
T2609	8157-8166	NN	denotes	progeroid
T2607	8167-8175	NNS	denotes	symptoms
T2610	8176-8177	-LRB-	denotes	[
T2612	8177-8179	CD	denotes	12
T2613	8179-8180	,	denotes	,
T2614	8180-8182	CD	denotes	13
T2615	8182-8183	,	denotes	,
T2616	8183-8185	CD	denotes	15
T2617	8185-8186	,	denotes	,
T2611	8186-8188	CD	denotes	23
T2618	8188-8189	-RRB-	denotes	]
T2619	8189-8191	,	denotes	,
T2620	8191-8193	PRP	denotes	we
T2621	8194-8198	RB	denotes	next
T2605	8199-8208	VBD	denotes	addressed
T2622	8209-8215	JJ	denotes	ageing
T2624	8215-8216	HYPH	denotes	-
T2623	8216-8223	JJ	denotes	related
T2625	8224-8234	NNS	denotes	parameters
T2626	8235-8237	IN	denotes	in
T2627	8238-8246	JJ	denotes	compound
T2629	8247-8259	JJ	denotes	heterozygous
T2628	8260-8264	NNS	denotes	mice
T2630	8265-8266	-LRB-	denotes	(
T2631	8266-8272	NN	denotes	Figure
T2632	8273-8274	CD	denotes	3
T2633	8274-8275	-RRB-	denotes	)
T2634	8275-8276	.	denotes	.
T2635	8276-8539	sentence	denotes	Whereas XpdTTD/TTD animals show reduced bone mineral density as an indication of the early onset of osteoporosis before ~14 mo of age [15], tail vertebrae from compound heterozygous XpdTTD/†XPCS mice were comparable to wt even at 20 mo of age (Figure 3B and 3C).
T2636	8277-8284	IN	denotes	Whereas
T2638	8285-8291	NN	denotes	XpdTTD
T2640	8291-8292	HYPH	denotes	/
T2639	8292-8295	NN	denotes	TTD
T2641	8296-8303	NNS	denotes	animals
T2637	8304-8308	VBP	denotes	show
T2643	8309-8316	VBN	denotes	reduced
T2645	8317-8321	NN	denotes	bone
T2646	8322-8329	NN	denotes	mineral
T2644	8330-8337	NN	denotes	density
T2647	8338-8340	IN	denotes	as
T2648	8341-8343	DT	denotes	an
T2649	8344-8354	NN	denotes	indication
T2650	8355-8357	IN	denotes	of
T2651	8358-8361	DT	denotes	the
T2653	8362-8367	JJ	denotes	early
T2652	8368-8373	NN	denotes	onset
T2654	8374-8376	IN	denotes	of
T2655	8377-8389	NN	denotes	osteoporosis
T2656	8390-8396	IN	denotes	before
T2657	8397-8398	SYM	denotes	~
T2658	8398-8400	CD	denotes	14
T2659	8401-8403	NNS	denotes	mo
T2660	8404-8406	IN	denotes	of
T2661	8407-8410	NN	denotes	age
T2662	8411-8412	-LRB-	denotes	[
T2663	8412-8414	CD	denotes	15
T2664	8414-8415	-RRB-	denotes	]
T2665	8415-8417	,	denotes	,
T2666	8417-8421	NN	denotes	tail
T2667	8422-8431	NNS	denotes	vertebrae
T2668	8432-8436	IN	denotes	from
T2669	8437-8445	NN	denotes	compound
T2671	8446-8458	JJ	denotes	heterozygous
T2672	8459-8465	NN	denotes	XpdTTD
T2674	8465-8466	HYPH	denotes	/
T2673	8466-8471	NN	denotes	†XPCS
T2670	8472-8476	NNS	denotes	mice
T2642	8477-8481	VBD	denotes	were
T2675	8482-8492	JJ	denotes	comparable
T2676	8493-8495	IN	denotes	to
T2677	8496-8498	NN	denotes	wt
T2678	8499-8503	RB	denotes	even
T2679	8504-8506	IN	denotes	at
T2680	8507-8509	CD	denotes	20
T2681	8510-8512	NNS	denotes	mo
T2682	8513-8515	IN	denotes	of
T2683	8516-8519	NN	denotes	age
T2684	8520-8521	-LRB-	denotes	(
T2686	8521-8527	NN	denotes	Figure
T2685	8528-8530	NN	denotes	3B
T2687	8531-8534	CC	denotes	and
T2688	8535-8537	NN	denotes	3C
T2689	8537-8538	-RRB-	denotes	)
T2690	8538-8539	.	denotes	.
T2691	8539-8711	sentence	denotes	Furthermore, whereas XpdTTD/TTD mice developed kyphosis earlier than wt animals (onset ~3 mo versus 12–20 mo), compound heterozygous XpdTTD/†XPCS mice did not (Figure 3B).
T2692	8540-8551	RB	denotes	Furthermore
T2694	8551-8553	,	denotes	,
T2695	8553-8560	IN	denotes	whereas
T2697	8561-8567	NN	denotes	XpdTTD
T2699	8567-8568	HYPH	denotes	/
T2698	8568-8571	NN	denotes	TTD
T2700	8572-8576	NNS	denotes	mice
T2696	8577-8586	VBD	denotes	developed
T2701	8587-8595	NN	denotes	kyphosis
T2702	8596-8603	RBR	denotes	earlier
T2703	8604-8608	IN	denotes	than
T2704	8609-8611	NN	denotes	wt
T2705	8612-8619	NNS	denotes	animals
T2706	8620-8621	-LRB-	denotes	(
T2707	8621-8626	NN	denotes	onset
T2708	8627-8628	SYM	denotes	~
T2709	8628-8629	CD	denotes	3
T2710	8630-8632	NNS	denotes	mo
T2711	8633-8639	CC	denotes	versus
T2712	8640-8642	CD	denotes	12
T2714	8642-8643	SYM	denotes	–
T2713	8643-8645	CD	denotes	20
T2715	8646-8648	NNS	denotes	mo
T2716	8648-8649	-RRB-	denotes	)
T2717	8649-8651	,	denotes	,
T2718	8651-8659	JJ	denotes	compound
T2720	8660-8672	JJ	denotes	heterozygous
T2721	8673-8679	NN	denotes	XpdTTD
T2723	8679-8680	HYPH	denotes	/
T2722	8680-8685	NN	denotes	†XPCS
T2719	8686-8690	NNS	denotes	mice
T2724	8691-8694	VBD	denotes	did
T2725	8695-8698	RB	denotes	not
T2726	8699-8700	-LRB-	denotes	(
T2727	8700-8706	NN	denotes	Figure
T2693	8707-8709	NN	denotes	3B
T2728	8709-8710	-RRB-	denotes	)
T2729	8710-8711	.	denotes	.
T2730	8711-8923	sentence	denotes	Overall appearance and body weight curves revealed that TTD-associated age-related premature cachexia and lack of general fitness were fully rescued in compound heterozygous XpdTTD/†XPCS mice (Figure 3A and 3D).
T2731	8712-8719	JJ	denotes	Overall
T2732	8720-8730	NN	denotes	appearance
T2734	8731-8734	CC	denotes	and
T2735	8735-8739	NN	denotes	body
T2736	8740-8746	NN	denotes	weight
T2737	8747-8753	NNS	denotes	curves
T2733	8754-8762	VBD	denotes	revealed
T2738	8763-8767	IN	denotes	that
T2740	8768-8771	NN	denotes	TTD
T2742	8771-8772	HYPH	denotes	-
T2741	8772-8782	JJ	denotes	associated
T2744	8783-8786	NN	denotes	age
T2746	8786-8787	HYPH	denotes	-
T2745	8787-8794	JJ	denotes	related
T2747	8795-8804	JJ	denotes	premature
T2743	8805-8813	NN	denotes	cachexia
T2748	8814-8817	CC	denotes	and
T2749	8818-8822	NN	denotes	lack
T2750	8823-8825	IN	denotes	of
T2751	8826-8833	JJ	denotes	general
T2752	8834-8841	NN	denotes	fitness
T2753	8842-8846	VBD	denotes	were
T2754	8847-8852	RB	denotes	fully
T2739	8853-8860	VBN	denotes	rescued
T2755	8861-8863	IN	denotes	in
T2756	8864-8872	JJ	denotes	compound
T2758	8873-8885	JJ	denotes	heterozygous
T2759	8886-8892	NN	denotes	XpdTTD
T2761	8892-8893	HYPH	denotes	/
T2760	8893-8898	NN	denotes	†XPCS
T2757	8899-8903	NNS	denotes	mice
T2762	8904-8905	-LRB-	denotes	(
T2764	8905-8911	NN	denotes	Figure
T2763	8912-8914	NN	denotes	3A
T2765	8915-8918	CC	denotes	and
T2766	8919-8921	NN	denotes	3D
T2767	8921-8922	-RRB-	denotes	)
T2768	8922-8923	.	denotes	.
T2769	8923-9024	sentence	denotes	Finally, the life span of compound heterozygotes was extended relative to XpdTTD/TTD mice (Table 2).
T2770	8924-8931	RB	denotes	Finally
T2772	8931-8933	,	denotes	,
T2773	8933-8936	DT	denotes	the
T2775	8937-8941	NN	denotes	life
T2774	8942-8946	NN	denotes	span
T2776	8947-8949	IN	denotes	of
T2777	8950-8958	JJ	denotes	compound
T2778	8959-8972	NNS	denotes	heterozygotes
T2779	8973-8976	VBD	denotes	was
T2771	8977-8985	VBN	denotes	extended
T2780	8986-8994	JJ	denotes	relative
T2781	8995-8997	IN	denotes	to
T2782	8998-9004	NN	denotes	XpdTTD
T2784	9004-9005	HYPH	denotes	/
T2783	9005-9008	NN	denotes	TTD
T2785	9009-9013	NNS	denotes	mice
T2786	9014-9015	-LRB-	denotes	(
T2787	9015-9020	NN	denotes	Table
T2788	9021-9022	CD	denotes	2
T2789	9022-9023	-RRB-	denotes	)
T2790	9023-9024	.	denotes	.
T2791	9024-10634	sentence	denotes	Figure 3 Rescue of TTD-Associated Segmental Progeroid Features in Compound Heterozygous Xpd TTD/†XPCS Mice (A) Photographs of 20-mo-old wt, compound heterozygous XpdTTD/†XPCS, and homozygous XpdTTD/TTD mice. Note the extreme cachexia (lack of subcutaneous fat) in the XpdTTD/TTD mouse and the absence of this phenotype in wt and XpdTTD/†XPCS mice. (B) Radiographs of 20-mo-old male wt, XpdTTD/†XPCS, and XpdTTD/TTD mice. Ageing XpdTTD/TTD mice develop kyphosis (curvature of the spinal column) and reduction of bone mineral density as shown in the 6–8 segment of the tail vertebrae counted from the pelvis (see close-up at right). Note the absence of these features in the XpdTTD / † XPCS mouse. (C) Quantification of relative bone mineral density of tail vertebrae from 20-mo-old male wt (n = 3), XpdTTD/†XPCS (n = 4), and XpdTTD/TTD (n = 3) mice. The p-values indicate the significance of the difference relative to XpdTTD/TTD. Error bars indicate SEM. (D) Body weight curves as a function of time. Note that the age-dependent cachexia observed in XpdTTD/TTD mice was rescued in both male and female XpdTTD / †XPCS mice. Significant differences between wt and XpdTTD/TTD but not between wt and XpdTTD/†XPCS mice were observed at 9 and 18 mo of age as indicated by asterisks. Error bars indicate SEM. Table 2 Pleiotropic Xpd Biallelic Effects in Mice and Cells To determine whether the homozygous lethal Xpd†XPCS allele was unique in its ability to ameliorate symptoms associated with the XpdTTD allele, we generated compound heterozygous XpdTTD/†XP mice by crossing the corresponding heterozygous animals.
T8041	9035-9041	NN	denotes	Rescue
T8042	9042-9044	IN	denotes	of
T8043	9045-9048	NN	denotes	TTD
T8045	9048-9049	HYPH	denotes	-
T8044	9049-9059	JJ	denotes	Associated
T8047	9060-9069	JJ	denotes	Segmental
T8048	9070-9079	JJ	denotes	Progeroid
T8046	9080-9088	NNS	denotes	Features
T8049	9089-9091	IN	denotes	in
T8050	9092-9100	JJ	denotes	Compound
T8052	9101-9113	JJ	denotes	Heterozygous
T8053	9114-9121	NN	denotes	Xpd TTD
T8055	9121-9122	HYPH	denotes	/
T8054	9122-9127	NN	denotes	†XPCS
T8051	9128-9132	NNS	denotes	Mice
T8056	9132-9233	sentence	denotes	(A) Photographs of 20-mo-old wt, compound heterozygous XpdTTD/†XPCS, and homozygous XpdTTD/TTD mice.
T8057	9133-9134	-LRB-	denotes	(
T8058	9134-9135	LS	denotes	A
T8060	9135-9136	-RRB-	denotes	)
T8059	9137-9148	NNS	denotes	Photographs
T8061	9149-9151	IN	denotes	of
T8062	9152-9154	CD	denotes	20
T8064	9154-9155	HYPH	denotes	-
T8063	9155-9157	NN	denotes	mo
T8066	9157-9158	HYPH	denotes	-
T8065	9158-9161	JJ	denotes	old
T8068	9162-9164	NN	denotes	wt
T8069	9164-9166	,	denotes	,
T8070	9166-9174	JJ	denotes	compound
T8072	9175-9187	JJ	denotes	heterozygous
T8073	9188-9194	NN	denotes	XpdTTD
T8074	9194-9195	HYPH	denotes	/
T8071	9195-9200	NN	denotes	†XPCS
T8075	9200-9202	,	denotes	,
T8076	9202-9205	CC	denotes	and
T8077	9206-9216	JJ	denotes	homozygous
T8079	9217-9223	NN	denotes	XpdTTD
T8080	9223-9224	HYPH	denotes	/
T8078	9224-9227	NN	denotes	TTD
T8067	9228-9232	NNS	denotes	mice
T8081	9232-9233	.	denotes	.
T8082	9233-9373	sentence	denotes	Note the extreme cachexia (lack of subcutaneous fat) in the XpdTTD/TTD mouse and the absence of this phenotype in wt and XpdTTD/†XPCS mice.
T8083	9234-9238	VB	denotes	Note
T8084	9239-9242	DT	denotes	the
T8086	9243-9250	JJ	denotes	extreme
T8085	9251-9259	NN	denotes	cachexia
T8087	9260-9261	-LRB-	denotes	(
T8088	9261-9265	NN	denotes	lack
T8089	9266-9268	IN	denotes	of
T8090	9269-9281	JJ	denotes	subcutaneous
T8091	9282-9285	NN	denotes	fat
T8092	9285-9286	-RRB-	denotes	)
T8093	9287-9289	IN	denotes	in
T8094	9290-9293	DT	denotes	the
T8096	9294-9300	NN	denotes	XpdTTD
T8098	9300-9301	HYPH	denotes	/
T8097	9301-9304	NN	denotes	TTD
T8095	9305-9310	NN	denotes	mouse
T8099	9311-9314	CC	denotes	and
T8100	9315-9318	DT	denotes	the
T8101	9319-9326	NN	denotes	absence
T8102	9327-9329	IN	denotes	of
T8103	9330-9334	DT	denotes	this
T8104	9335-9344	NN	denotes	phenotype
T8105	9345-9347	IN	denotes	in
T8106	9348-9350	NN	denotes	wt
T8108	9351-9354	CC	denotes	and
T8109	9355-9361	NN	denotes	XpdTTD
T8111	9361-9362	HYPH	denotes	/
T8110	9362-9367	NN	denotes	†XPCS
T8107	9368-9372	NNS	denotes	mice
T8112	9372-9373	.	denotes	.
T8113	9373-9446	sentence	denotes	(B) Radiographs of 20-mo-old male wt, XpdTTD/†XPCS, and XpdTTD/TTD mice.
T8114	9374-9375	-LRB-	denotes	(
T8115	9375-9376	LS	denotes	B
T8117	9376-9377	-RRB-	denotes	)
T8116	9378-9389	NNS	denotes	Radiographs
T8118	9390-9392	IN	denotes	of
T8119	9393-9395	CD	denotes	20
T8121	9395-9396	HYPH	denotes	-
T8120	9396-9398	NN	denotes	mo
T8123	9398-9399	HYPH	denotes	-
T8122	9399-9402	JJ	denotes	old
T8125	9403-9407	JJ	denotes	male
T8126	9408-9410	NN	denotes	wt
T8127	9410-9412	,	denotes	,
T8128	9412-9418	NN	denotes	XpdTTD
T8130	9418-9419	HYPH	denotes	/
T8129	9419-9424	NN	denotes	†XPCS
T8131	9424-9426	,	denotes	,
T8132	9426-9429	CC	denotes	and
T8133	9430-9436	NN	denotes	XpdTTD
T8135	9436-9437	HYPH	denotes	/
T8134	9437-9440	NN	denotes	TTD
T8124	9441-9445	NNS	denotes	mice
T8136	9445-9446	.	denotes	.
T8137	9446-9656	sentence	denotes	Ageing XpdTTD/TTD mice develop kyphosis (curvature of the spinal column) and reduction of bone mineral density as shown in the 6–8 segment of the tail vertebrae counted from the pelvis (see close-up at right).
T8138	9447-9453	VBG	denotes	Ageing
T8140	9454-9460	NN	denotes	XpdTTD
T8142	9460-9461	HYPH	denotes	/
T8141	9461-9464	NN	denotes	TTD
T8139	9465-9469	NNS	denotes	mice
T8143	9470-9477	VBP	denotes	develop
T8144	9478-9486	NN	denotes	kyphosis
T8145	9487-9488	-LRB-	denotes	(
T8146	9488-9497	NN	denotes	curvature
T8147	9498-9500	IN	denotes	of
T8148	9501-9504	DT	denotes	the
T8150	9505-9511	JJ	denotes	spinal
T8149	9512-9518	NN	denotes	column
T8151	9518-9519	-RRB-	denotes	)
T8152	9520-9523	CC	denotes	and
T8153	9524-9533	NN	denotes	reduction
T8154	9534-9536	IN	denotes	of
T8155	9537-9541	NN	denotes	bone
T8156	9542-9549	NN	denotes	mineral
T8157	9550-9557	NN	denotes	density
T8158	9558-9560	IN	denotes	as
T8159	9561-9566	VBN	denotes	shown
T8160	9567-9569	IN	denotes	in
T8161	9570-9573	DT	denotes	the
T8163	9574-9575	CD	denotes	6
T8165	9575-9576	SYM	denotes	–
T8164	9576-9577	CD	denotes	8
T8162	9578-9585	NN	denotes	segment
T8166	9586-9588	IN	denotes	of
T8167	9589-9592	DT	denotes	the
T8169	9593-9597	NN	denotes	tail
T8168	9598-9607	NNS	denotes	vertebrae
T8170	9608-9615	VBN	denotes	counted
T8171	9616-9620	IN	denotes	from
T8172	9621-9624	DT	denotes	the
T8173	9625-9631	NN	denotes	pelvis
T8174	9632-9633	-LRB-	denotes	(
T8175	9633-9636	VB	denotes	see
T8176	9637-9642	JJ	denotes	close
T8177	9642-9643	HYPH	denotes	-
T8178	9643-9645	RP	denotes	up
T8179	9646-9648	IN	denotes	at
T8180	9649-9654	NN	denotes	right
T8181	9654-9655	-RRB-	denotes	)
T8182	9655-9656	.	denotes	.
T8183	9656-9721	sentence	denotes	Note the absence of these features in the XpdTTD / † XPCS mouse.
T8184	9657-9661	VB	denotes	Note
T8185	9662-9665	DT	denotes	the
T8186	9666-9673	NN	denotes	absence
T8187	9674-9676	IN	denotes	of
T8188	9677-9682	DT	denotes	these
T8189	9683-9691	NNS	denotes	features
T8190	9692-9694	IN	denotes	in
T8191	9695-9698	DT	denotes	the
T8193	9699-9706	NN	denotes	XpdTTD
T8195	9706-9707	HYPH	denotes	/
T8194	9707-9714	NN	denotes	† XPCS
T8192	9715-9720	NN	denotes	mouse
T8196	9720-9721	.	denotes	.
T8197	9721-9874	sentence	denotes	(C) Quantification of relative bone mineral density of tail vertebrae from 20-mo-old male wt (n = 3), XpdTTD/†XPCS (n = 4), and XpdTTD/TTD (n = 3) mice.
T8198	9722-9723	-LRB-	denotes	(
T8199	9723-9724	LS	denotes	C
T8201	9724-9725	-RRB-	denotes	)
T8200	9726-9740	NN	denotes	Quantification
T8202	9741-9743	IN	denotes	of
T8203	9744-9752	JJ	denotes	relative
T8205	9753-9757	NN	denotes	bone
T8206	9758-9765	NN	denotes	mineral
T8204	9766-9773	NN	denotes	density
T8207	9774-9776	IN	denotes	of
T8208	9777-9781	NN	denotes	tail
T8209	9782-9791	NNS	denotes	vertebrae
T8210	9792-9796	IN	denotes	from
T8211	9797-9799	CD	denotes	20
T8213	9799-9800	HYPH	denotes	-
T8212	9800-9802	NN	denotes	mo
T8215	9802-9803	HYPH	denotes	-
T8214	9803-9806	JJ	denotes	old
T8217	9807-9811	JJ	denotes	male
T8218	9812-9814	NN	denotes	wt
T8219	9815-9816	-LRB-	denotes	(
T8221	9816-9817	NN	denotes	n
T8222	9818-9819	SYM	denotes	=
T8220	9820-9821	CD	denotes	3
T8223	9821-9822	-RRB-	denotes	)
T8224	9822-9824	,	denotes	,
T8225	9824-9830	NN	denotes	XpdTTD
T8227	9830-9831	HYPH	denotes	/
T8226	9831-9836	NN	denotes	†XPCS
T8228	9837-9838	-LRB-	denotes	(
T8230	9838-9839	NN	denotes	n
T8231	9840-9841	SYM	denotes	=
T8229	9842-9843	CD	denotes	4
T8232	9843-9844	-RRB-	denotes	)
T8233	9844-9846	,	denotes	,
T8234	9846-9849	CC	denotes	and
T8235	9850-9856	NN	denotes	XpdTTD
T8237	9856-9857	HYPH	denotes	/
T8236	9857-9860	NN	denotes	TTD
T8238	9861-9862	-LRB-	denotes	(
T8240	9862-9863	NN	denotes	n
T8241	9864-9865	SYM	denotes	=
T8239	9866-9867	CD	denotes	3
T8242	9867-9868	-RRB-	denotes	)
T8216	9869-9873	NNS	denotes	mice
T8243	9873-9874	.	denotes	.
T8244	9874-9955	sentence	denotes	The p-values indicate the significance of the difference relative to XpdTTD/TTD.
T8245	9875-9878	DT	denotes	The
T8247	9879-9880	NN	denotes	p
T8248	9880-9881	HYPH	denotes	-
T8246	9881-9887	NNS	denotes	values
T8249	9888-9896	VBP	denotes	indicate
T8250	9897-9900	DT	denotes	the
T8251	9901-9913	NN	denotes	significance
T8252	9914-9916	IN	denotes	of
T8253	9917-9920	DT	denotes	the
T8254	9921-9931	NN	denotes	difference
T8255	9932-9940	JJ	denotes	relative
T8256	9941-9943	IN	denotes	to
T8257	9944-9950	NN	denotes	XpdTTD
T8259	9950-9951	HYPH	denotes	/
T8258	9951-9954	NN	denotes	TTD
T8260	9954-9955	.	denotes	.
T8261	9955-9980	sentence	denotes	Error bars indicate SEM.
T8262	9956-9961	NN	denotes	Error
T8263	9962-9966	NNS	denotes	bars
T8264	9967-9975	VBP	denotes	indicate
T8265	9976-9979	NN	denotes	SEM
T8266	9979-9980	.	denotes	.
T8267	9980-10026	sentence	denotes	(D) Body weight curves as a function of time.
T8268	9981-9982	-LRB-	denotes	(
T8269	9982-9983	LS	denotes	D
T8271	9983-9984	-RRB-	denotes	)
T8272	9985-9989	NN	denotes	Body
T8273	9990-9996	NN	denotes	weight
T8270	9997-10003	NNS	denotes	curves
T8274	10004-10006	IN	denotes	as
T8275	10007-10008	DT	denotes	a
T8276	10009-10017	NN	denotes	function
T8277	10018-10020	IN	denotes	of
T8278	10021-10025	NN	denotes	time
T8279	10025-10026	.	denotes	.
T8280	10026-10148	sentence	denotes	Note that the age-dependent cachexia observed in XpdTTD/TTD mice was rescued in both male and female XpdTTD / †XPCS mice.
T8281	10027-10031	VB	denotes	Note
T8282	10032-10036	IN	denotes	that
T8284	10037-10040	DT	denotes	the
T8286	10041-10044	NN	denotes	age
T8288	10044-10045	HYPH	denotes	-
T8287	10045-10054	JJ	denotes	dependent
T8285	10055-10063	NN	denotes	cachexia
T8289	10064-10072	VBN	denotes	observed
T8290	10073-10075	IN	denotes	in
T8291	10076-10082	NN	denotes	XpdTTD
T8293	10082-10083	HYPH	denotes	/
T8292	10083-10086	NN	denotes	TTD
T8294	10087-10091	NNS	denotes	mice
T8295	10092-10095	VBD	denotes	was
T8283	10096-10103	VBN	denotes	rescued
T8296	10104-10106	IN	denotes	in
T8297	10107-10111	CC	denotes	both
T8298	10112-10116	JJ	denotes	male
T8300	10117-10120	CC	denotes	and
T8301	10121-10127	JJ	denotes	female
T8302	10128-10135	NN	denotes	XpdTTD
T8304	10135-10136	HYPH	denotes	/
T8303	10136-10142	NN	denotes	†XPCS
T8299	10143-10147	NNS	denotes	mice
T8305	10147-10148	.	denotes	.
T8306	10148-10302	sentence	denotes	Significant differences between wt and XpdTTD/TTD but not between wt and XpdTTD/†XPCS mice were observed at 9 and 18 mo of age as indicated by asterisks.
T8307	10149-10160	JJ	denotes	Significant
T8308	10161-10172	NNS	denotes	differences
T8310	10173-10180	IN	denotes	between
T8311	10181-10183	NN	denotes	wt
T8312	10184-10187	CC	denotes	and
T8313	10188-10194	NN	denotes	XpdTTD
T8315	10194-10195	HYPH	denotes	/
T8314	10195-10198	NN	denotes	TTD
T8316	10199-10202	CC	denotes	but
T8317	10203-10206	RB	denotes	not
T8318	10207-10214	IN	denotes	between
T8319	10215-10217	NN	denotes	wt
T8321	10218-10221	CC	denotes	and
T8322	10222-10228	NN	denotes	XpdTTD
T8324	10228-10229	HYPH	denotes	/
T8323	10229-10234	NN	denotes	†XPCS
T8320	10235-10239	NNS	denotes	mice
T8325	10240-10244	VBD	denotes	were
T8309	10245-10253	VBN	denotes	observed
T8326	10254-10256	IN	denotes	at
T8327	10257-10258	CD	denotes	9
T8329	10259-10262	CC	denotes	and
T8330	10263-10265	CD	denotes	18
T8328	10266-10268	NNS	denotes	mo
T8331	10269-10271	IN	denotes	of
T8332	10272-10275	NN	denotes	age
T8333	10276-10278	IN	denotes	as
T8334	10279-10288	VBN	denotes	indicated
T8335	10289-10291	IN	denotes	by
T8336	10292-10301	NNS	denotes	asterisks
T8337	10301-10302	.	denotes	.
T8338	10302-10327	sentence	denotes	Error bars indicate SEM.
T8339	10303-10308	NN	denotes	Error
T8340	10309-10313	NNS	denotes	bars
T8341	10314-10322	VBP	denotes	indicate
T8342	10323-10326	NN	denotes	SEM
T8343	10326-10327	.	denotes	.
T9241	10337-10348	JJ	denotes	Pleiotropic
T9243	10349-10352	NN	denotes	Xpd
T9244	10353-10362	JJ	denotes	Biallelic
T9242	10363-10370	NNS	denotes	Effects
T9245	10371-10373	IN	denotes	in
T9246	10374-10378	NNS	denotes	Mice
T9247	10379-10382	CC	denotes	and
T9248	10383-10388	NNS	denotes	Cells
T2792	10389-10391	TO	denotes	To
T2793	10392-10401	VB	denotes	determine
T2795	10402-10409	IN	denotes	whether
T2797	10410-10413	DT	denotes	the
T2799	10414-10424	JJ	denotes	homozygous
T2800	10425-10431	JJ	denotes	lethal
T2801	10432-10440	NN	denotes	Xpd†XPCS
T2798	10441-10447	NN	denotes	allele
T2796	10448-10451	VBD	denotes	was
T2802	10452-10458	JJ	denotes	unique
T2803	10459-10461	IN	denotes	in
T2804	10462-10465	PRP$	denotes	its
T2805	10466-10473	NN	denotes	ability
T2806	10474-10476	TO	denotes	to
T2807	10477-10487	VB	denotes	ameliorate
T2808	10488-10496	NNS	denotes	symptoms
T2809	10497-10507	VBN	denotes	associated
T2810	10508-10512	IN	denotes	with
T2811	10513-10516	DT	denotes	the
T2813	10517-10523	NN	denotes	XpdTTD
T2812	10524-10530	NN	denotes	allele
T2814	10530-10532	,	denotes	,
T2815	10532-10534	PRP	denotes	we
T2794	10535-10544	VBD	denotes	generated
T2816	10545-10553	NN	denotes	compound
T2818	10554-10566	JJ	denotes	heterozygous
T2819	10567-10573	NN	denotes	XpdTTD
T2821	10573-10574	HYPH	denotes	/
T2820	10574-10577	NN	denotes	†XP
T2817	10578-10582	NNS	denotes	mice
T2822	10583-10585	IN	denotes	by
T2823	10586-10594	VBG	denotes	crossing
T2824	10595-10598	DT	denotes	the
T2826	10599-10612	VBG	denotes	corresponding
T2827	10613-10625	JJ	denotes	heterozygous
T2825	10626-10633	NNS	denotes	animals
T2828	10633-10634	.	denotes	.
T2829	10634-11080	sentence	denotes	Similar to the Xpd †XPCS allele, the homozygous lethal Xpd †XP allele rescued cutaneous symptoms including hair loss (except locally during the first round; unpublished data), reduced cysteine content (cysteine index 9.3 ± 0.9 standard deviation [87% of wt], p = 0.01 versus TTD), ageing-associated premature cachexia (males and females were 36.1 ± 6.4 g [93% of wt] and 39.2 ± 3.2 g [116% of wt], respectively), and reduced life span (Table 2).
T2830	10635-10642	JJ	denotes	Similar
T2832	10643-10645	IN	denotes	to
T2833	10646-10649	DT	denotes	the
T2835	10650-10659	NN	denotes	Xpd †XPCS
T2834	10660-10666	NN	denotes	allele
T2836	10666-10668	,	denotes	,
T2837	10668-10671	DT	denotes	the
T2839	10672-10682	JJ	denotes	homozygous
T2840	10683-10689	JJ	denotes	lethal
T2841	10690-10697	NN	denotes	Xpd †XP
T2838	10698-10704	NN	denotes	allele
T2831	10705-10712	VBD	denotes	rescued
T2842	10713-10722	JJ	denotes	cutaneous
T2843	10723-10731	NNS	denotes	symptoms
T2844	10732-10741	VBG	denotes	including
T2845	10742-10746	NN	denotes	hair
T2846	10747-10751	NN	denotes	loss
T2847	10752-10753	-LRB-	denotes	(
T2849	10753-10759	IN	denotes	except
T2850	10760-10767	RB	denotes	locally
T2851	10768-10774	IN	denotes	during
T2852	10775-10778	DT	denotes	the
T2854	10779-10784	JJ	denotes	first
T2853	10785-10790	NN	denotes	round
T2855	10790-10791	:	denotes	;
T2856	10792-10803	JJ	denotes	unpublished
T2848	10804-10808	NNS	denotes	data
T2857	10808-10809	-RRB-	denotes	)
T2858	10809-10811	,	denotes	,
T2859	10811-10818	VBN	denotes	reduced
T2861	10819-10827	NN	denotes	cysteine
T2860	10828-10835	NN	denotes	content
T2862	10836-10837	-LRB-	denotes	(
T2864	10837-10845	NN	denotes	cysteine
T2863	10846-10851	NN	denotes	index
T2865	10852-10855	CD	denotes	9.3
T2866	10856-10857	SYM	denotes	±
T2867	10858-10861	CD	denotes	0.9
T2869	10862-10870	JJ	denotes	standard
T2868	10871-10880	NN	denotes	deviation
T2870	10881-10882	-LRB-	denotes	[
T2872	10882-10884	CD	denotes	87
T2871	10884-10885	NN	denotes	%
T2873	10886-10888	IN	denotes	of
T2874	10889-10891	NN	denotes	wt
T2875	10891-10892	-RRB-	denotes	]
T2876	10892-10894	,	denotes	,
T2877	10894-10895	NN	denotes	p
T2879	10896-10897	SYM	denotes	=
T2880	10898-10902	CD	denotes	0.01
T2881	10903-10909	CC	denotes	versus
T2878	10910-10913	NN	denotes	TTD
T2882	10913-10914	-RRB-	denotes	)
T2883	10914-10916	,	denotes	,
T2884	10916-10922	NN	denotes	ageing
T2886	10922-10923	HYPH	denotes	-
T2885	10923-10933	VBN	denotes	associated
T2888	10934-10943	JJ	denotes	premature
T2887	10944-10952	NN	denotes	cachexia
T2889	10953-10954	-LRB-	denotes	(
T2891	10954-10959	NNS	denotes	males
T2892	10960-10963	CC	denotes	and
T2893	10964-10971	NNS	denotes	females
T2890	10972-10976	VBD	denotes	were
T2894	10977-10981	CD	denotes	36.1
T2896	10982-10983	SYM	denotes	±
T2895	10984-10987	CD	denotes	6.4
T2897	10988-10989	NNS	denotes	g
T2898	10990-10991	-LRB-	denotes	[
T2900	10991-10993	CD	denotes	93
T2899	10993-10994	NN	denotes	%
T2901	10995-10997	IN	denotes	of
T2902	10998-11000	NN	denotes	wt
T2903	11000-11001	-RRB-	denotes	]
T2904	11002-11005	CC	denotes	and
T2905	11006-11010	CD	denotes	39.2
T2907	11011-11012	SYM	denotes	±
T2906	11013-11016	CD	denotes	3.2
T2908	11017-11018	NNS	denotes	g
T2909	11019-11020	-LRB-	denotes	[
T2911	11020-11023	CD	denotes	116
T2910	11023-11024	NN	denotes	%
T2912	11025-11027	IN	denotes	of
T2913	11028-11030	NN	denotes	wt
T2914	11030-11031	-RRB-	denotes	]
T2915	11031-11033	,	denotes	,
T2916	11033-11045	RB	denotes	respectively
T2917	11045-11046	-RRB-	denotes	)
T2918	11046-11048	,	denotes	,
T2919	11048-11051	CC	denotes	and
T2920	11052-11059	VBN	denotes	reduced
T2922	11060-11064	NN	denotes	life
T2921	11065-11069	NN	denotes	span
T2923	11070-11071	-LRB-	denotes	(
T2924	11071-11076	NN	denotes	Table
T2925	11077-11078	CD	denotes	2
T2926	11078-11079	-RRB-	denotes	)
T2927	11079-11080	.	denotes	.
T2928	11080-11289	sentence	denotes	Taken together, these data indicate that two independent alleles, which on their own are unable to support viability (Table 1), were nonetheless able to ameliorate TTD-associated phenotypes in vivo (Table 2).
T2929	11081-11086	VBN	denotes	Taken
T2931	11087-11095	RB	denotes	together
T2932	11095-11097	,	denotes	,
T2933	11097-11102	DT	denotes	these
T2934	11103-11107	NNS	denotes	data
T2930	11108-11116	VBP	denotes	indicate
T2935	11117-11121	IN	denotes	that
T2937	11122-11125	CD	denotes	two
T2939	11126-11137	JJ	denotes	independent
T2938	11138-11145	NNS	denotes	alleles
T2940	11145-11147	,	denotes	,
T2941	11147-11152	WDT	denotes	which
T2943	11153-11155	IN	denotes	on
T2944	11156-11161	PRP$	denotes	their
T2945	11162-11165	NN	denotes	own
T2942	11166-11169	VBP	denotes	are
T2946	11170-11176	JJ	denotes	unable
T2947	11177-11179	TO	denotes	to
T2948	11180-11187	VB	denotes	support
T2949	11188-11197	NN	denotes	viability
T2950	11198-11199	-LRB-	denotes	(
T2951	11199-11204	NN	denotes	Table
T2952	11205-11206	CD	denotes	1
T2953	11206-11207	-RRB-	denotes	)
T2954	11207-11209	,	denotes	,
T2936	11209-11213	VBD	denotes	were
T2955	11214-11225	RB	denotes	nonetheless
T2956	11226-11230	JJ	denotes	able
T2957	11231-11233	TO	denotes	to
T2958	11234-11244	VB	denotes	ameliorate
T2959	11245-11248	NN	denotes	TTD
T2961	11248-11249	HYPH	denotes	-
T2960	11249-11259	VBN	denotes	associated
T2962	11260-11270	NNS	denotes	phenotypes
T2963	11271-11273	FW	denotes	in
T2964	11274-11278	FW	denotes	vivo
T2965	11279-11280	-LRB-	denotes	(
T2966	11280-11285	NN	denotes	Table
T2967	11286-11287	CD	denotes	2
T2968	11287-11288	-RRB-	denotes	)
T2969	11288-11289	.	denotes	.
T3312	11291-11300	JJ	denotes	Molecular
T3313	11301-11311	NNS	denotes	Mechanisms
T3314	11312-11314	IN	denotes	of
T3315	11315-11324	JJ	denotes	Biallelic
T3316	11325-11332	NNS	denotes	Effects
T3317	11332-11631	sentence	denotes	We next turned to UV-based cellular assays including unscheduled DNA synthesis after UV irradiation (UV-UDS), recovery of RNA synthesis after UV irradiation (UV-RRS), and UV survival, which report on the NER subpathways (global genome NER and transcription-coupled NER) and total NER, respectively.
T3318	11333-11335	PRP	denotes	We
T3320	11336-11340	RB	denotes	next
T3319	11341-11347	VBD	denotes	turned
T3321	11348-11350	IN	denotes	to
T3322	11351-11353	NN	denotes	UV
T3324	11353-11354	HYPH	denotes	-
T3323	11354-11359	VBN	denotes	based
T3326	11360-11368	JJ	denotes	cellular
T3325	11369-11375	NNS	denotes	assays
T3327	11376-11385	VBG	denotes	including
T3328	11386-11397	JJ	denotes	unscheduled
T3330	11398-11401	NN	denotes	DNA
T3329	11402-11411	NN	denotes	synthesis
T3331	11412-11417	IN	denotes	after
T3332	11418-11420	NN	denotes	UV
T3333	11421-11432	NN	denotes	irradiation
T3334	11433-11434	-LRB-	denotes	(
T3336	11434-11436	NN	denotes	UV
T3337	11436-11437	HYPH	denotes	-
T3335	11437-11440	NN	denotes	UDS
T3338	11440-11441	-RRB-	denotes	)
T3339	11441-11443	,	denotes	,
T3340	11443-11451	NN	denotes	recovery
T3341	11452-11454	IN	denotes	of
T3342	11455-11458	NN	denotes	RNA
T3343	11459-11468	NN	denotes	synthesis
T3344	11469-11474	IN	denotes	after
T3345	11475-11477	NN	denotes	UV
T3346	11478-11489	NN	denotes	irradiation
T3347	11490-11491	-LRB-	denotes	(
T3349	11491-11493	NN	denotes	UV
T3350	11493-11494	HYPH	denotes	-
T3348	11494-11497	NN	denotes	RRS
T3351	11497-11498	-RRB-	denotes	)
T3352	11498-11500	,	denotes	,
T3353	11500-11503	CC	denotes	and
T3354	11504-11506	NN	denotes	UV
T3355	11507-11515	NN	denotes	survival
T3356	11515-11517	,	denotes	,
T3357	11517-11522	WDT	denotes	which
T3358	11523-11529	VBP	denotes	report
T3359	11530-11532	IN	denotes	on
T3360	11533-11536	DT	denotes	the
T3362	11537-11540	NN	denotes	NER
T3361	11541-11552	NNS	denotes	subpathways
T3363	11553-11554	-LRB-	denotes	(
T3365	11554-11560	JJ	denotes	global
T3366	11561-11567	NN	denotes	genome
T3364	11568-11571	NN	denotes	NER
T3367	11572-11575	CC	denotes	and
T3368	11576-11589	NN	denotes	transcription
T3370	11589-11590	HYPH	denotes	-
T3369	11590-11597	VBN	denotes	coupled
T3371	11598-11601	NN	denotes	NER
T3372	11601-11602	-RRB-	denotes	)
T3373	11603-11606	CC	denotes	and
T3374	11607-11612	JJ	denotes	total
T3375	11613-11616	NN	denotes	NER
T3376	11616-11618	,	denotes	,
T3377	11618-11630	RB	denotes	respectively
T3378	11630-11631	.	denotes	.
T3379	11631-11757	sentence	denotes	In none of these assays was the response to UV improved in compound heterozygotes relative to TTD homozygotes (Figure 4A–4C).
T3380	11632-11634	IN	denotes	In
T3382	11635-11639	NN	denotes	none
T3383	11640-11642	IN	denotes	of
T3384	11643-11648	DT	denotes	these
T3385	11649-11655	NNS	denotes	assays
T3386	11656-11659	VBD	denotes	was
T3387	11660-11663	DT	denotes	the
T3388	11664-11672	NN	denotes	response
T3389	11673-11675	IN	denotes	to
T3390	11676-11678	NN	denotes	UV
T3381	11679-11687	VBN	denotes	improved
T3391	11688-11690	IN	denotes	in
T3392	11691-11699	NN	denotes	compound
T3393	11700-11713	NNS	denotes	heterozygotes
T3394	11714-11722	JJ	denotes	relative
T3395	11723-11725	IN	denotes	to
T3396	11726-11729	NN	denotes	TTD
T3397	11730-11741	NNS	denotes	homozygotes
T3398	11742-11743	-LRB-	denotes	(
T3400	11743-11749	NN	denotes	Figure
T3399	11750-11752	NN	denotes	4A
T3401	11752-11753	SYM	denotes	–
T3402	11753-11755	NN	denotes	4C
T3403	11755-11756	-RRB-	denotes	)
T3404	11756-11757	.	denotes	.
T3405	11757-12103	sentence	denotes	However, unlike the in vivo TTD phenotypes described above, in which XpdTTD/TTD and XpdTTD/KO animals were indistinguishable, XpdTTD dosage effects were observed in UV survival, UV-UDS, and UV-RRS, indicating that cellular parameters as measured in fibroblasts here do not always correlate with the phenotype at the level of the intact organism.
T3406	11758-11765	RB	denotes	However
T3408	11765-11767	,	denotes	,
T3409	11767-11773	IN	denotes	unlike
T3410	11774-11777	DT	denotes	the
T3412	11778-11780	FW	denotes	in
T3413	11781-11785	FW	denotes	vivo
T3414	11786-11789	NN	denotes	TTD
T3411	11790-11800	NNS	denotes	phenotypes
T3415	11801-11810	VBN	denotes	described
T3416	11811-11816	RB	denotes	above
T3417	11816-11818	,	denotes	,
T3418	11818-11820	IN	denotes	in
T3420	11821-11826	WDT	denotes	which
T3421	11827-11833	NN	denotes	XpdTTD
T3423	11833-11834	HYPH	denotes	/
T3422	11834-11837	NN	denotes	TTD
T3425	11838-11841	CC	denotes	and
T3426	11842-11848	NN	denotes	XpdTTD
T3428	11848-11849	HYPH	denotes	/
T3427	11849-11851	NN	denotes	KO
T3424	11852-11859	NNS	denotes	animals
T3419	11860-11864	VBD	denotes	were
T3429	11865-11882	JJ	denotes	indistinguishable
T3430	11882-11884	,	denotes	,
T3431	11884-11890	NN	denotes	XpdTTD
T3433	11891-11897	NN	denotes	dosage
T3432	11898-11905	NNS	denotes	effects
T3434	11906-11910	VBD	denotes	were
T3407	11911-11919	VBN	denotes	observed
T3435	11920-11922	IN	denotes	in
T3436	11923-11925	NN	denotes	UV
T3437	11926-11934	NN	denotes	survival
T3438	11934-11936	,	denotes	,
T3439	11936-11938	NN	denotes	UV
T3441	11938-11939	HYPH	denotes	-
T3440	11939-11942	NN	denotes	UDS
T3442	11942-11944	,	denotes	,
T3443	11944-11947	CC	denotes	and
T3444	11948-11950	NN	denotes	UV
T3446	11950-11951	HYPH	denotes	-
T3445	11951-11954	NN	denotes	RRS
T3447	11954-11956	,	denotes	,
T3448	11956-11966	VBG	denotes	indicating
T3449	11967-11971	IN	denotes	that
T3451	11972-11980	JJ	denotes	cellular
T3452	11981-11991	NNS	denotes	parameters
T3453	11992-11994	IN	denotes	as
T3454	11995-12003	VBN	denotes	measured
T3455	12004-12006	IN	denotes	in
T3456	12007-12018	NNS	denotes	fibroblasts
T3457	12019-12023	RB	denotes	here
T3458	12024-12026	VBP	denotes	do
T3459	12027-12030	RB	denotes	not
T3460	12031-12037	RB	denotes	always
T3450	12038-12047	VB	denotes	correlate
T3461	12048-12052	IN	denotes	with
T3462	12053-12056	DT	denotes	the
T3463	12057-12066	NN	denotes	phenotype
T3464	12067-12069	IN	denotes	at
T3465	12070-12073	DT	denotes	the
T3466	12074-12079	NN	denotes	level
T3467	12080-12082	IN	denotes	of
T3468	12083-12086	DT	denotes	the
T3470	12087-12093	JJ	denotes	intact
T3469	12094-12102	NN	denotes	organism
T3471	12102-12103	.	denotes	.
T3472	12103-12226	sentence	denotes	XpdTTD/KO hemizygous cells were thus used as the baseline on which to compare the activity of compound heterozygous cells.
T3473	12104-12110	NN	denotes	XpdTTD
T3475	12110-12111	HYPH	denotes	/
T3474	12111-12113	NN	denotes	KO
T3477	12114-12124	JJ	denotes	hemizygous
T3476	12125-12130	NNS	denotes	cells
T3479	12131-12135	VBD	denotes	were
T3480	12136-12140	RB	denotes	thus
T3478	12141-12145	VBN	denotes	used
T3481	12146-12148	IN	denotes	as
T3482	12149-12152	DT	denotes	the
T3483	12153-12161	NN	denotes	baseline
T3484	12162-12164	IN	denotes	on
T3486	12165-12170	WDT	denotes	which
T3487	12171-12173	TO	denotes	to
T3485	12174-12181	VB	denotes	compare
T3488	12182-12185	DT	denotes	the
T3489	12186-12194	NN	denotes	activity
T3490	12195-12197	IN	denotes	of
T3491	12198-12206	NN	denotes	compound
T3493	12207-12219	JJ	denotes	heterozygous
T3492	12220-12225	NNS	denotes	cells
T3494	12225-12226	.	denotes	.
T3495	12226-12433	sentence	denotes	Relative to XpdTTD/KO hemizygote cells, UV survival was improved by the homozygous lethal Xpd†XPCS allele in XpdTTD/†XPCS compound heterozygous cells and to a lesser degree by the Xpd†XP allele (Figure 4A).
T3496	12227-12235	JJ	denotes	Relative
T3498	12236-12238	IN	denotes	to
T3499	12239-12245	NN	denotes	XpdTTD
T3501	12245-12246	HYPH	denotes	/
T3500	12246-12248	NN	denotes	KO
T3503	12249-12259	NN	denotes	hemizygote
T3502	12260-12265	NNS	denotes	cells
T3504	12265-12267	,	denotes	,
T3505	12267-12269	NN	denotes	UV
T3506	12270-12278	NN	denotes	survival
T3507	12279-12282	VBD	denotes	was
T3497	12283-12291	VBN	denotes	improved
T3508	12292-12294	IN	denotes	by
T3509	12295-12298	DT	denotes	the
T3511	12299-12309	JJ	denotes	homozygous
T3512	12310-12316	JJ	denotes	lethal
T3513	12317-12325	NN	denotes	Xpd†XPCS
T3510	12326-12332	NN	denotes	allele
T3514	12333-12335	IN	denotes	in
T3515	12336-12342	NN	denotes	XpdTTD
T3517	12342-12343	HYPH	denotes	/
T3516	12343-12348	NN	denotes	†XPCS
T3519	12349-12357	NN	denotes	compound
T3520	12358-12370	JJ	denotes	heterozygous
T3518	12371-12376	NNS	denotes	cells
T3521	12377-12380	CC	denotes	and
T3522	12381-12383	IN	denotes	to
T3524	12384-12385	DT	denotes	a
T3526	12386-12392	JJR	denotes	lesser
T3525	12393-12399	NN	denotes	degree
T3523	12400-12402	IN	denotes	by
T3527	12403-12406	DT	denotes	the
T3529	12407-12413	NN	denotes	Xpd†XP
T3528	12414-12420	NN	denotes	allele
T3530	12421-12422	-LRB-	denotes	(
T3532	12422-12428	NN	denotes	Figure
T3531	12429-12431	NN	denotes	4A
T3533	12431-12432	-RRB-	denotes	)
T3534	12432-12433	.	denotes	.
T3535	12433-12573	sentence	denotes	Because of embryonic and cellular lethality, we were unable to test UV survival associated exclusively with the Xpd†XPCS or Xpd†XP alleles.
T3536	12434-12441	IN	denotes	Because
T3538	12442-12444	IN	denotes	of
T3539	12445-12454	JJ	denotes	embryonic
T3541	12455-12458	CC	denotes	and
T3542	12459-12467	JJ	denotes	cellular
T3540	12468-12477	NN	denotes	lethality
T3543	12477-12479	,	denotes	,
T3544	12479-12481	PRP	denotes	we
T3537	12482-12486	VBD	denotes	were
T3545	12487-12493	JJ	denotes	unable
T3546	12494-12496	TO	denotes	to
T3547	12497-12501	VB	denotes	test
T3548	12502-12504	NN	denotes	UV
T3549	12505-12513	NN	denotes	survival
T3550	12514-12524	VBN	denotes	associated
T3551	12525-12536	RB	denotes	exclusively
T3552	12537-12541	IN	denotes	with
T3553	12542-12545	DT	denotes	the
T3555	12546-12554	NN	denotes	Xpd†XPCS
T3556	12555-12557	CC	denotes	or
T3557	12558-12564	NN	denotes	Xpd†XP
T3554	12565-12572	NNS	denotes	alleles
T3558	12572-12573	.	denotes	.
T3559	12573-12815	sentence	denotes	However, homozygous XPDXP (XPDR683W) and hemizygous XPDXPCS (XPDG602D) human cells are known to be highly sensitive to UV [19,25], as are cells from a homozygous viable XpdXPCS/XPCS (XPDG602D/G602D) mouse model (Figure 4A, dotted line) [23].
T3560	12574-12581	RB	denotes	However
T3562	12581-12583	,	denotes	,
T3563	12583-12593	JJ	denotes	homozygous
T3564	12594-12599	NN	denotes	XPDXP
T3566	12600-12601	-LRB-	denotes	(
T3567	12601-12609	NN	denotes	XPDR683W
T3568	12609-12610	-RRB-	denotes	)
T3569	12611-12614	CC	denotes	and
T3570	12615-12625	JJ	denotes	hemizygous
T3571	12626-12633	NN	denotes	XPDXPCS
T3572	12634-12635	-LRB-	denotes	(
T3573	12635-12643	NN	denotes	XPDG602D
T3574	12643-12644	-RRB-	denotes	)
T3575	12645-12650	JJ	denotes	human
T3565	12651-12656	NNS	denotes	cells
T3576	12657-12660	VBP	denotes	are
T3561	12661-12666	VBN	denotes	known
T3577	12667-12669	TO	denotes	to
T3578	12670-12672	VB	denotes	be
T3579	12673-12679	RB	denotes	highly
T3580	12680-12689	JJ	denotes	sensitive
T3581	12690-12692	IN	denotes	to
T3582	12693-12695	NN	denotes	UV
T3583	12696-12697	-LRB-	denotes	[
T3585	12697-12699	CD	denotes	19
T3586	12699-12700	,	denotes	,
T3584	12700-12702	CD	denotes	25
T3587	12702-12703	-RRB-	denotes	]
T3588	12703-12705	,	denotes	,
T3589	12705-12707	IN	denotes	as
T3590	12708-12711	VBP	denotes	are
T3591	12712-12717	NNS	denotes	cells
T3592	12718-12722	IN	denotes	from
T3593	12723-12724	DT	denotes	a
T3595	12725-12735	JJ	denotes	homozygous
T3596	12736-12742	JJ	denotes	viable
T3597	12743-12750	NN	denotes	XpdXPCS
T3599	12750-12751	HYPH	denotes	/
T3598	12751-12755	NN	denotes	XPCS
T3600	12756-12757	-LRB-	denotes	(
T3602	12757-12765	NN	denotes	XPDG602D
T3603	12765-12766	HYPH	denotes	/
T3601	12766-12771	NN	denotes	G602D
T3604	12771-12772	-RRB-	denotes	)
T3605	12773-12778	NN	denotes	mouse
T3594	12779-12784	NN	denotes	model
T3606	12785-12786	-LRB-	denotes	(
T3608	12786-12792	NN	denotes	Figure
T3607	12793-12795	NN	denotes	4A
T3609	12795-12797	,	denotes	,
T3610	12797-12803	VBN	denotes	dotted
T3611	12804-12808	NN	denotes	line
T3612	12808-12809	-RRB-	denotes	)
T3613	12810-12811	-LRB-	denotes	[
T3614	12811-12813	CD	denotes	23
T3615	12813-12814	-RRB-	denotes	]
T3616	12814-12815	.	denotes	.
T3617	12815-12976	sentence	denotes	Thus, the survival of XpdTTD/†XPCS (and XpdTTD/†XP) cells likely represents a level of UV resistance that neither mutant allele can impart on its own (Table 2).
T3618	12816-12820	RB	denotes	Thus
T3620	12820-12822	,	denotes	,
T3621	12822-12825	DT	denotes	the
T3622	12826-12834	NN	denotes	survival
T3623	12835-12837	IN	denotes	of
T3624	12838-12844	NN	denotes	XpdTTD
T3626	12844-12845	HYPH	denotes	/
T3625	12845-12850	NN	denotes	†XPCS
T3628	12851-12852	-LRB-	denotes	(
T3629	12852-12855	CC	denotes	and
T3630	12856-12862	NN	denotes	XpdTTD
T3632	12862-12863	HYPH	denotes	/
T3631	12863-12866	NN	denotes	†XP
T3633	12866-12867	-RRB-	denotes	)
T3627	12868-12873	NNS	denotes	cells
T3634	12874-12880	RB	denotes	likely
T3619	12881-12891	VBZ	denotes	represents
T3635	12892-12893	DT	denotes	a
T3636	12894-12899	NN	denotes	level
T3637	12900-12902	IN	denotes	of
T3638	12903-12905	NN	denotes	UV
T3639	12906-12916	NN	denotes	resistance
T3640	12917-12921	WDT	denotes	that
T3642	12922-12929	CC	denotes	neither
T3644	12930-12936	JJ	denotes	mutant
T3643	12937-12943	NN	denotes	allele
T3645	12944-12947	MD	denotes	can
T3641	12948-12954	VB	denotes	impart
T3646	12955-12957	IN	denotes	on
T3647	12958-12961	PRP$	denotes	its
T3648	12962-12965	NN	denotes	own
T3649	12966-12967	-LRB-	denotes	(
T3650	12967-12972	NN	denotes	Table
T3651	12973-12974	CD	denotes	2
T3652	12974-12975	-RRB-	denotes	)
T3653	12975-12976	.	denotes	.
T3654	12976-13134	sentence	denotes	Significant effects of compound heterozygosity on NER subpathways relative to XpdTTD/KO cells were observed in XpdTTD/†XP cells but only for UV-UDS activity.
T3655	12977-12988	JJ	denotes	Significant
T3656	12989-12996	NNS	denotes	effects
T3658	12997-12999	IN	denotes	of
T3659	13000-13008	NN	denotes	compound
T3660	13009-13023	NN	denotes	heterozygosity
T3661	13024-13026	IN	denotes	on
T3662	13027-13030	NN	denotes	NER
T3663	13031-13042	NNS	denotes	subpathways
T3664	13043-13051	JJ	denotes	relative
T3665	13052-13054	IN	denotes	to
T3666	13055-13061	NN	denotes	XpdTTD
T3668	13061-13062	HYPH	denotes	/
T3667	13062-13064	NN	denotes	KO
T3669	13065-13070	NNS	denotes	cells
T3670	13071-13075	VBD	denotes	were
T3657	13076-13084	VBN	denotes	observed
T3671	13085-13087	IN	denotes	in
T3672	13088-13094	NN	denotes	XpdTTD
T3674	13094-13095	HYPH	denotes	/
T3673	13095-13098	NN	denotes	†XP
T3675	13099-13104	NNS	denotes	cells
T3676	13105-13108	CC	denotes	but
T3677	13109-13113	RB	denotes	only
T3678	13114-13117	IN	denotes	for
T3679	13118-13120	NN	denotes	UV
T3681	13120-13121	HYPH	denotes	-
T3680	13121-13124	NN	denotes	UDS
T3682	13125-13133	NN	denotes	activity
T3683	13133-13134	.	denotes	.
T3684	13134-13383	sentence	denotes	Finally, none of the mutant TFIIH combinations (carrying alterations associated with TTD [XPDR722W], XPCS [XPDG602D], or XP [XPDR683W]) exhibited synergism in an in vitro NER reaction reconstituted with different mutant TFIIH complexes (Figure 4D).
T3685	13135-13142	RB	denotes	Finally
T3687	13142-13144	,	denotes	,
T3688	13144-13148	NN	denotes	none
T3689	13149-13151	IN	denotes	of
T3690	13152-13155	DT	denotes	the
T3692	13156-13162	JJ	denotes	mutant
T3693	13163-13168	NN	denotes	TFIIH
T3691	13169-13181	NNS	denotes	combinations
T3694	13182-13183	-LRB-	denotes	(
T3695	13183-13191	VBG	denotes	carrying
T3696	13192-13203	NNS	denotes	alterations
T3697	13204-13214	VBN	denotes	associated
T3698	13215-13219	IN	denotes	with
T3699	13220-13223	NN	denotes	TTD
T3700	13224-13225	-LRB-	denotes	[
T3701	13225-13233	NN	denotes	XPDR722W
T3702	13233-13234	-RRB-	denotes	]
T3703	13234-13236	,	denotes	,
T3704	13236-13240	NN	denotes	XPCS
T3705	13241-13242	-LRB-	denotes	[
T3706	13242-13250	NN	denotes	XPDG602D
T3707	13250-13251	-RRB-	denotes	]
T3708	13251-13253	,	denotes	,
T3709	13253-13255	CC	denotes	or
T3710	13256-13258	NN	denotes	XP
T3711	13259-13260	-LRB-	denotes	[
T3712	13260-13268	NN	denotes	XPDR683W
T3713	13268-13269	-RRB-	denotes	]
T3714	13269-13270	-RRB-	denotes	)
T3686	13271-13280	VBD	denotes	exhibited
T3715	13281-13290	NN	denotes	synergism
T3716	13291-13293	IN	denotes	in
T3717	13294-13296	DT	denotes	an
T3719	13297-13299	FW	denotes	in
T3720	13300-13305	FW	denotes	vitro
T3721	13306-13309	NN	denotes	NER
T3718	13310-13318	NN	denotes	reaction
T3722	13319-13332	VBN	denotes	reconstituted
T3723	13333-13337	IN	denotes	with
T3724	13338-13347	JJ	denotes	different
T3726	13348-13354	JJ	denotes	mutant
T3727	13355-13360	NN	denotes	TFIIH
T3725	13361-13370	NNS	denotes	complexes
T3728	13371-13372	-LRB-	denotes	(
T3730	13372-13378	NN	denotes	Figure
T3729	13379-13381	NN	denotes	4D
T3731	13381-13382	-RRB-	denotes	)
T3732	13382-13383	.	denotes	.
T3733	13383-13618	sentence	denotes	Taken together, these data are consistent with interallelic complementation of UV sensitivity in cells but underscore the lack of any correlation between UV-related repair characteristics and TTD progeroid phenotypes in animal models.
T3734	13384-13389	VBN	denotes	Taken
T3736	13390-13398	RB	denotes	together
T3737	13398-13400	,	denotes	,
T3738	13400-13405	DT	denotes	these
T3739	13406-13410	NNS	denotes	data
T3735	13411-13414	VBP	denotes	are
T3740	13415-13425	JJ	denotes	consistent
T3741	13426-13430	IN	denotes	with
T3742	13431-13443	JJ	denotes	interallelic
T3743	13444-13459	NN	denotes	complementation
T3744	13460-13462	IN	denotes	of
T3745	13463-13465	NN	denotes	UV
T3746	13466-13477	NN	denotes	sensitivity
T3747	13478-13480	IN	denotes	in
T3748	13481-13486	NNS	denotes	cells
T3749	13487-13490	CC	denotes	but
T3750	13491-13501	VBP	denotes	underscore
T3751	13502-13505	DT	denotes	the
T3752	13506-13510	NN	denotes	lack
T3753	13511-13513	IN	denotes	of
T3754	13514-13517	DT	denotes	any
T3755	13518-13529	NN	denotes	correlation
T3756	13530-13537	IN	denotes	between
T3757	13538-13540	NN	denotes	UV
T3759	13540-13541	HYPH	denotes	-
T3758	13541-13548	VBN	denotes	related
T3761	13549-13555	NN	denotes	repair
T3760	13556-13571	NNS	denotes	characteristics
T3762	13572-13575	CC	denotes	and
T3763	13576-13579	NN	denotes	TTD
T3765	13580-13589	NN	denotes	progeroid
T3764	13590-13600	NNS	denotes	phenotypes
T3766	13601-13603	IN	denotes	in
T3767	13604-13610	NN	denotes	animal
T3768	13611-13617	NNS	denotes	models
T3769	13617-13618	.	denotes	.
T3770	13618-13619	sentence	denotes
T8564	13629-13634	NN	denotes	TFIIH
T8565	13635-13644	NNS	denotes	Functions
T8566	13645-13648	CC	denotes	and
T8567	13649-13659	NNS	denotes	Mechanisms
T8568	13660-13662	IN	denotes	of
T8569	13663-13666	NN	denotes	XPD
T8571	13666-13667	HYPH	denotes	-
T8570	13667-13677	VBN	denotes	Associated
T8573	13678-13685	NN	denotes	Disease
T8572	13686-13696	NN	denotes	Pleiotropy
T8574	13696-13740	sentence	denotes	(A) Cellular survival after UV irradiation.
T8575	13697-13698	-LRB-	denotes	(
T8576	13698-13699	LS	denotes	A
T8578	13699-13700	-RRB-	denotes	)
T8579	13701-13709	JJ	denotes	Cellular
T8577	13710-13718	NN	denotes	survival
T8580	13719-13724	IN	denotes	after
T8581	13725-13727	NN	denotes	UV
T8582	13728-13739	NN	denotes	irradiation
T8583	13739-13740	.	denotes	.
T8584	13740-13866	sentence	denotes	Rescue of hemizygous XpdTTD/KO survival by Xpd†XPCS and Xpd†XP alleles is illustrated by arrows marked A and B, respectively.
T8585	13741-13747	NN	denotes	Rescue
T8587	13748-13750	IN	denotes	of
T8588	13751-13761	JJ	denotes	hemizygous
T8590	13762-13768	NN	denotes	XpdTTD
T8592	13768-13769	HYPH	denotes	/
T8591	13769-13771	NN	denotes	KO
T8589	13772-13780	NN	denotes	survival
T8593	13781-13783	IN	denotes	by
T8594	13784-13792	NN	denotes	Xpd†XPCS
T8596	13793-13796	CC	denotes	and
T8597	13797-13803	NN	denotes	Xpd†XP
T8595	13804-13811	NNS	denotes	alleles
T8598	13812-13814	VBZ	denotes	is
T8586	13815-13826	VBN	denotes	illustrated
T8599	13827-13829	IN	denotes	by
T8600	13830-13836	NNS	denotes	arrows
T8601	13837-13843	VBN	denotes	marked
T8602	13844-13845	NN	denotes	A
T8603	13846-13849	CC	denotes	and
T8604	13850-13851	NN	denotes	B
T8605	13851-13853	,	denotes	,
T8606	13853-13865	RB	denotes	respectively
T8607	13865-13866	.	denotes	.
T8608	13866-14004	sentence	denotes	UV survival of homozygous XpdXPCS/XPCS cells (asterisk) from the normally expressed viable allele (XpdXPCS) is depicted by a dotted line.
T8609	13867-13869	NN	denotes	UV
T8610	13870-13878	NN	denotes	survival
T8612	13879-13881	IN	denotes	of
T8613	13882-13892	JJ	denotes	homozygous
T8615	13893-13900	NN	denotes	XpdXPCS
T8617	13900-13901	HYPH	denotes	/
T8616	13901-13905	NN	denotes	XPCS
T8614	13906-13911	NNS	denotes	cells
T8618	13912-13913	-LRB-	denotes	(
T8619	13913-13921	NN	denotes	asterisk
T8620	13921-13922	-RRB-	denotes	)
T8621	13923-13927	IN	denotes	from
T8622	13928-13931	DT	denotes	the
T8624	13932-13940	RB	denotes	normally
T8625	13941-13950	VBN	denotes	expressed
T8626	13951-13957	JJ	denotes	viable
T8623	13958-13964	NN	denotes	allele
T8627	13965-13966	-LRB-	denotes	(
T8628	13966-13973	NN	denotes	XpdXPCS
T8629	13973-13974	-RRB-	denotes	)
T8630	13975-13977	VBZ	denotes	is
T8611	13978-13986	VBN	denotes	depicted
T8631	13987-13989	IN	denotes	by
T8632	13990-13991	DT	denotes	a
T8634	13992-13998	VBN	denotes	dotted
T8633	13999-14003	NN	denotes	line
T8635	14003-14004	.	denotes	.
T8636	14004-14136	sentence	denotes	Survival curves represent an average of four independent experiments; 1–2 cell lines per genotype were included in each experiment.
T8637	14005-14013	NN	denotes	Survival
T8638	14014-14020	NNS	denotes	curves
T8639	14021-14030	VBP	denotes	represent
T8641	14031-14033	DT	denotes	an
T8642	14034-14041	NN	denotes	average
T8643	14042-14044	IN	denotes	of
T8644	14045-14049	CD	denotes	four
T8646	14050-14061	JJ	denotes	independent
T8645	14062-14073	NNS	denotes	experiments
T8647	14073-14074	:	denotes	;
T8648	14075-14076	CD	denotes	1
T8650	14076-14077	SYM	denotes	–
T8649	14077-14078	CD	denotes	2
T8652	14079-14083	NN	denotes	cell
T8651	14084-14089	NNS	denotes	lines
T8653	14090-14093	IN	denotes	per
T8654	14094-14102	NN	denotes	genotype
T8655	14103-14107	VBD	denotes	were
T8640	14108-14116	VBN	denotes	included
T8656	14117-14119	IN	denotes	in
T8657	14120-14124	DT	denotes	each
T8658	14125-14135	NN	denotes	experiment
T8659	14135-14136	.	denotes	.
T8660	14136-14181	sentence	denotes	Error bars indicate SEM between experiments.
T8661	14137-14142	NN	denotes	Error
T8662	14143-14147	NNS	denotes	bars
T8663	14148-14156	VBP	denotes	indicate
T8664	14157-14160	NN	denotes	SEM
T8665	14161-14168	IN	denotes	between
T8666	14169-14180	NNS	denotes	experiments
T8667	14180-14181	.	denotes	.
T8668	14181-14228	sentence	denotes	(B) UV-UDS, a measure of global genome repair.
T8669	14182-14183	-LRB-	denotes	(
T8670	14183-14184	LS	denotes	B
T8672	14184-14185	-RRB-	denotes	)
T8673	14186-14188	NN	denotes	UV
T8674	14188-14189	HYPH	denotes	-
T8671	14189-14192	NN	denotes	UDS
T8675	14192-14194	,	denotes	,
T8676	14194-14195	DT	denotes	a
T8677	14196-14203	NN	denotes	measure
T8678	14204-14206	IN	denotes	of
T8679	14207-14213	JJ	denotes	global
T8681	14214-14220	NN	denotes	genome
T8680	14221-14227	NN	denotes	repair
T8682	14227-14228	.	denotes	.
T8683	14228-14397	sentence	denotes	Number of experiments: n = 15 (XpdTTD/TTD), n = 6 (XpdTTD/KO), n = 4 (XpdTTD/†XPCS ), n = 2 (XpdTTD/†XP ); 1–2 cell lines per genotype were included in each experiment.
T8684	14229-14235	NN	denotes	Number
T8686	14236-14238	IN	denotes	of
T8687	14239-14250	NNS	denotes	experiments
T8688	14250-14252	:	denotes	:
T8689	14252-14253	NN	denotes	n
T8691	14254-14255	SYM	denotes	=
T8690	14256-14258	CD	denotes	15
T8692	14259-14260	-LRB-	denotes	(
T8693	14260-14266	NN	denotes	XpdTTD
T8694	14266-14267	SYM	denotes	/
T8695	14267-14270	NN	denotes	TTD
T8696	14270-14271	-RRB-	denotes	)
T8697	14271-14273	,	denotes	,
T8698	14273-14274	NN	denotes	n
T8700	14275-14276	SYM	denotes	=
T8699	14277-14278	CD	denotes	6
T8701	14279-14280	-LRB-	denotes	(
T8703	14280-14286	NN	denotes	XpdTTD
T8704	14286-14287	HYPH	denotes	/
T8702	14287-14289	NN	denotes	KO
T8705	14289-14290	-RRB-	denotes	)
T8706	14290-14292	,	denotes	,
T8707	14292-14293	NN	denotes	n
T8709	14294-14295	SYM	denotes	=
T8708	14296-14297	CD	denotes	4
T8710	14298-14299	-LRB-	denotes	(
T8712	14299-14305	NN	denotes	XpdTTD
T8713	14305-14306	HYPH	denotes	/
T8711	14306-14311	NN	denotes	†XPCS
T8714	14312-14313	-RRB-	denotes	)
T8715	14313-14315	,	denotes	,
T8716	14315-14316	NN	denotes	n
T8718	14317-14318	SYM	denotes	=
T8717	14319-14320	CD	denotes	2
T8719	14321-14322	-LRB-	denotes	(
T8721	14322-14328	NN	denotes	XpdTTD
T8722	14328-14329	HYPH	denotes	/
T8720	14329-14332	NN	denotes	†XP
T8723	14333-14334	-RRB-	denotes	)
T8724	14334-14335	:	denotes	;
T8725	14336-14337	CD	denotes	1
T8727	14337-14338	SYM	denotes	–
T8726	14338-14339	CD	denotes	2
T8729	14340-14344	NN	denotes	cell
T8728	14345-14350	NNS	denotes	lines
T8730	14351-14354	IN	denotes	per
T8731	14355-14363	NN	denotes	genotype
T8732	14364-14368	VBD	denotes	were
T8685	14369-14377	VBN	denotes	included
T8733	14378-14380	IN	denotes	in
T8734	14381-14385	DT	denotes	each
T8735	14386-14396	NN	denotes	experiment
T8736	14396-14397	.	denotes	.
T8737	14397-14517	sentence	denotes	The asterisk indicates significant difference with XpdTTD/TTD; crosses indicate significant differences with XpdTTD/KO.
T8738	14398-14401	DT	denotes	The
T8739	14402-14410	NN	denotes	asterisk
T8740	14411-14420	VBZ	denotes	indicates
T8742	14421-14432	JJ	denotes	significant
T8743	14433-14443	NN	denotes	difference
T8744	14444-14448	IN	denotes	with
T8745	14449-14455	NN	denotes	XpdTTD
T8747	14455-14456	HYPH	denotes	/
T8746	14456-14459	NN	denotes	TTD
T8748	14459-14460	:	denotes	;
T8749	14461-14468	NNS	denotes	crosses
T8741	14469-14477	VBP	denotes	indicate
T8750	14478-14489	JJ	denotes	significant
T8751	14490-14501	NNS	denotes	differences
T8752	14502-14506	IN	denotes	with
T8753	14507-14513	NN	denotes	XpdTTD
T8755	14513-14514	HYPH	denotes	/
T8754	14514-14516	NN	denotes	KO
T8756	14516-14517	.	denotes	.
T8757	14517-14594	sentence	denotes	(C) UV-RRS, a measure of transcription-coupled repair of UV-induced lesions.
T8758	14518-14519	-LRB-	denotes	(
T8759	14519-14520	LS	denotes	C
T8761	14520-14521	-RRB-	denotes	)
T8762	14522-14524	NN	denotes	UV
T8763	14524-14525	HYPH	denotes	-
T8760	14525-14528	NN	denotes	RRS
T8764	14528-14530	,	denotes	,
T8765	14530-14531	DT	denotes	a
T8766	14532-14539	NN	denotes	measure
T8767	14540-14542	IN	denotes	of
T8768	14543-14556	NN	denotes	transcription
T8770	14556-14557	HYPH	denotes	-
T8769	14557-14564	VBN	denotes	coupled
T8771	14565-14571	NN	denotes	repair
T8772	14572-14574	IN	denotes	of
T8773	14575-14577	NN	denotes	UV
T8775	14577-14578	HYPH	denotes	-
T8774	14578-14585	VBN	denotes	induced
T8776	14586-14593	NNS	denotes	lesions
T8777	14593-14594	.	denotes	.
T8778	14594-14762	sentence	denotes	Number of experiments: n = 7 (XpdTTD/TTD), n = 2 (XpdTTD/KO), n = 4 (XpdTTD/†XPCS ), n = 2 (XpdTTD/†XP ); 1–2 cell lines per genotype were included in each experiment.
T8779	14595-14601	NN	denotes	Number
T8781	14602-14604	IN	denotes	of
T8782	14605-14616	NNS	denotes	experiments
T8783	14616-14618	:	denotes	:
T8784	14618-14619	NN	denotes	n
T8786	14620-14621	SYM	denotes	=
T8785	14622-14623	CD	denotes	7
T8787	14624-14625	-LRB-	denotes	(
T8789	14625-14631	NN	denotes	XpdTTD
T8790	14631-14632	HYPH	denotes	/
T8788	14632-14635	NN	denotes	TTD
T8791	14635-14636	-RRB-	denotes	)
T8792	14636-14638	,	denotes	,
T8793	14638-14639	NN	denotes	n
T8795	14640-14641	SYM	denotes	=
T8794	14642-14643	CD	denotes	2
T8796	14644-14645	-LRB-	denotes	(
T8798	14645-14651	NN	denotes	XpdTTD
T8799	14651-14652	HYPH	denotes	/
T8797	14652-14654	NN	denotes	KO
T8800	14654-14655	-RRB-	denotes	)
T8801	14655-14657	,	denotes	,
T8802	14657-14658	NN	denotes	n
T8804	14659-14660	SYM	denotes	=
T8803	14661-14662	CD	denotes	4
T8805	14663-14664	-LRB-	denotes	(
T8807	14664-14670	NN	denotes	XpdTTD
T8808	14670-14671	HYPH	denotes	/
T8806	14671-14676	NN	denotes	†XPCS
T8809	14677-14678	-RRB-	denotes	)
T8810	14678-14680	,	denotes	,
T8811	14680-14681	NN	denotes	n
T8813	14682-14683	SYM	denotes	=
T8812	14684-14685	CD	denotes	2
T8814	14686-14687	-LRB-	denotes	(
T8816	14687-14693	NN	denotes	XpdTTD
T8817	14693-14694	HYPH	denotes	/
T8815	14694-14697	NN	denotes	†XP
T8818	14698-14699	-RRB-	denotes	)
T8819	14699-14700	:	denotes	;
T8820	14701-14702	CD	denotes	1
T8822	14702-14703	SYM	denotes	–
T8821	14703-14704	CD	denotes	2
T8824	14705-14709	NN	denotes	cell
T8823	14710-14715	NNS	denotes	lines
T8825	14716-14719	IN	denotes	per
T8826	14720-14728	NN	denotes	genotype
T8827	14729-14733	VBD	denotes	were
T8780	14734-14742	VBN	denotes	included
T8828	14743-14745	IN	denotes	in
T8829	14746-14750	DT	denotes	each
T8830	14751-14761	NN	denotes	experiment
T8831	14761-14762	.	denotes	.
T8832	14762-14869	sentence	denotes	(D) Incision/excision activity of combinations of altered TFIIH complexes in a reconstituted NER reaction.
T8833	14763-14764	-LRB-	denotes	(
T8834	14764-14765	LS	denotes	D
T8836	14765-14766	-RRB-	denotes	)
T8837	14767-14775	NN	denotes	Incision
T8839	14775-14776	HYPH	denotes	/
T8838	14776-14784	NN	denotes	excision
T8835	14785-14793	NN	denotes	activity
T8840	14794-14796	IN	denotes	of
T8841	14797-14809	NNS	denotes	combinations
T8842	14810-14812	IN	denotes	of
T8843	14813-14820	VBN	denotes	altered
T8845	14821-14826	NN	denotes	TFIIH
T8844	14827-14836	NNS	denotes	complexes
T8846	14837-14839	IN	denotes	in
T8847	14840-14841	DT	denotes	a
T8849	14842-14855	VBN	denotes	reconstituted
T8850	14856-14859	NN	denotes	NER
T8848	14860-14868	NN	denotes	reaction
T8851	14868-14869	.	denotes	.
T8852	14869-15123	sentence	denotes	Equal amounts of single or mixed populations of recombinant TFIIHs (containing XPD, XPB, p62, p52, His-p44, Flag-p34, cdk7, cyclin H, Mat1, and p8) were mixed with recombinant XPG, XPF/ERCC1, XPC/hHR23B, RPA, and a radiolabelled synthetic NER substrate.
T8853	14870-14875	JJ	denotes	Equal
T8854	14876-14883	NNS	denotes	amounts
T8856	14884-14886	IN	denotes	of
T8857	14887-14893	JJ	denotes	single
T8859	14894-14896	CC	denotes	or
T8860	14897-14902	JJ	denotes	mixed
T8858	14903-14914	NNS	denotes	populations
T8861	14915-14917	IN	denotes	of
T8862	14918-14929	JJ	denotes	recombinant
T8863	14930-14936	NNS	denotes	TFIIHs
T8864	14937-14938	-LRB-	denotes	(
T8865	14938-14948	VBG	denotes	containing
T8866	14949-14952	NN	denotes	XPD
T8867	14952-14954	,	denotes	,
T8868	14954-14957	NN	denotes	XPB
T8869	14957-14959	,	denotes	,
T8870	14959-14962	NN	denotes	p62
T8871	14962-14964	,	denotes	,
T8872	14964-14967	NN	denotes	p52
T8873	14967-14969	,	denotes	,
T8874	14969-14972	NN	denotes	His
T8876	14972-14973	HYPH	denotes	-
T8875	14973-14976	NN	denotes	p44
T8877	14976-14978	,	denotes	,
T8878	14978-14982	NN	denotes	Flag
T8880	14982-14983	HYPH	denotes	-
T8879	14983-14986	NN	denotes	p34
T8881	14986-14988	,	denotes	,
T8882	14988-14992	NN	denotes	cdk7
T8883	14992-14994	,	denotes	,
T8884	14994-15000	NN	denotes	cyclin
T8885	15001-15002	NN	denotes	H
T8886	15002-15004	,	denotes	,
T8887	15004-15008	NN	denotes	Mat1
T8888	15008-15010	,	denotes	,
T8889	15010-15013	CC	denotes	and
T8890	15014-15016	NN	denotes	p8
T8891	15016-15017	-RRB-	denotes	)
T8892	15018-15022	VBD	denotes	were
T8855	15023-15028	VBN	denotes	mixed
T8893	15029-15033	IN	denotes	with
T8894	15034-15045	JJ	denotes	recombinant
T8895	15046-15049	NN	denotes	XPG
T8896	15049-15051	,	denotes	,
T8897	15051-15054	NN	denotes	XPF
T8899	15054-15055	HYPH	denotes	/
T8898	15055-15060	NN	denotes	ERCC1
T8900	15060-15062	,	denotes	,
T8901	15062-15065	NN	denotes	XPC
T8903	15065-15066	HYPH	denotes	/
T8902	15066-15072	NN	denotes	hHR23B
T8904	15072-15074	,	denotes	,
T8905	15074-15077	NN	denotes	RPA
T8906	15077-15079	,	denotes	,
T8907	15079-15082	CC	denotes	and
T8908	15083-15084	DT	denotes	a
T8910	15085-15098	VBN	denotes	radiolabelled
T8911	15099-15108	JJ	denotes	synthetic
T8912	15109-15112	NN	denotes	NER
T8909	15113-15122	NN	denotes	substrate
T8913	15122-15123	.	denotes	.
T8914	15123-15266	sentence	denotes	The excision products (26–34 nucleotides in length) were visualised at nucleotide resolution on a denaturing polyacrylamide gel as indicated .
T8915	15124-15127	DT	denotes	The
T8917	15128-15136	NN	denotes	excision
T8916	15137-15145	NNS	denotes	products
T8919	15146-15147	-LRB-	denotes	(
T8921	15147-15149	CD	denotes	26
T8923	15149-15150	SYM	denotes	–
T8922	15150-15152	CD	denotes	34
T8920	15153-15164	NNS	denotes	nucleotides
T8924	15165-15167	IN	denotes	in
T8925	15168-15174	NN	denotes	length
T8926	15174-15175	-RRB-	denotes	)
T8927	15176-15180	VBD	denotes	were
T8918	15181-15191	VBN	denotes	visualised
T8928	15192-15194	IN	denotes	at
T8929	15195-15205	NN	denotes	nucleotide
T8930	15206-15216	NN	denotes	resolution
T8931	15217-15219	IN	denotes	on
T8932	15220-15221	DT	denotes	a
T8934	15222-15232	VBG	denotes	denaturing
T8935	15233-15247	NN	denotes	polyacrylamide
T8933	15248-15251	NN	denotes	gel
T8936	15252-15254	IN	denotes	as
T8937	15255-15264	VBN	denotes	indicated
T8938	15265-15266	.	denotes	.
T8939	15266-15417	sentence	denotes	Note the weak activity corresponding to each single and combined TFIIH complex (lanes 3–8) relative to the wt (lane 1) and negative controls (lane 2).
T8940	15267-15271	VB	denotes	Note
T8941	15272-15275	DT	denotes	the
T8943	15276-15280	JJ	denotes	weak
T8942	15281-15289	NN	denotes	activity
T8944	15290-15303	VBG	denotes	corresponding
T8945	15304-15306	IN	denotes	to
T8946	15307-15311	DT	denotes	each
T8948	15312-15318	JJ	denotes	single
T8949	15319-15322	CC	denotes	and
T8950	15323-15331	VBN	denotes	combined
T8951	15332-15337	NN	denotes	TFIIH
T8947	15338-15345	NN	denotes	complex
T8952	15346-15347	-LRB-	denotes	(
T8954	15347-15352	NNS	denotes	lanes
T8953	15353-15354	CD	denotes	3
T8955	15354-15355	SYM	denotes	–
T8956	15355-15356	CD	denotes	8
T8957	15356-15357	-RRB-	denotes	)
T8958	15358-15366	JJ	denotes	relative
T8959	15367-15369	IN	denotes	to
T8960	15370-15373	DT	denotes	the
T8961	15374-15376	NN	denotes	wt
T8962	15377-15378	-LRB-	denotes	(
T8963	15378-15382	NN	denotes	lane
T8964	15383-15384	CD	denotes	1
T8965	15384-15385	-RRB-	denotes	)
T8966	15386-15389	CC	denotes	and
T8967	15390-15398	JJ	denotes	negative
T8968	15399-15407	NNS	denotes	controls
T8969	15408-15409	-LRB-	denotes	(
T8970	15409-15413	NN	denotes	lane
T8971	15414-15415	CD	denotes	2
T8972	15415-15416	-RRB-	denotes	)
T8973	15416-15417	.	denotes	.
T8974	15417-15630	sentence	denotes	(E) Xpd dose-dependent reduction of TFIIH in homozygous XpdTTD/TTD, hemizygous XpdTTD/KO, and compound heterozygous XpdTTD/†XPCS and XpdTTD/†XP cells by comparative immunofluorescence of the p62 subunit of TFIIH.
T8975	15418-15419	-LRB-	denotes	(
T8976	15419-15420	LS	denotes	E
T8978	15420-15421	-RRB-	denotes	)
T8979	15422-15425	NN	denotes	Xpd
T8980	15426-15430	NN	denotes	dose
T8982	15430-15431	HYPH	denotes	-
T8981	15431-15440	JJ	denotes	dependent
T8977	15441-15450	NN	denotes	reduction
T8983	15451-15453	IN	denotes	of
T8984	15454-15459	NN	denotes	TFIIH
T8985	15460-15462	IN	denotes	in
T8986	15463-15473	JJ	denotes	homozygous
T8988	15474-15480	NN	denotes	XpdTTD
T8989	15480-15481	HYPH	denotes	/
T8987	15481-15484	NN	denotes	TTD
T8991	15484-15486	,	denotes	,
T8992	15486-15496	JJ	denotes	hemizygous
T8994	15497-15503	NN	denotes	XpdTTD
T8995	15503-15504	HYPH	denotes	/
T8993	15504-15506	NN	denotes	KO
T8996	15506-15508	,	denotes	,
T8997	15508-15511	CC	denotes	and
T8998	15512-15520	NN	denotes	compound
T9000	15521-15533	JJ	denotes	heterozygous
T9001	15534-15540	NN	denotes	XpdTTD
T9002	15540-15541	HYPH	denotes	/
T8999	15541-15546	NN	denotes	†XPCS
T9003	15547-15550	CC	denotes	and
T9004	15551-15557	NN	denotes	XpdTTD
T9006	15557-15558	HYPH	denotes	/
T9005	15558-15561	NN	denotes	†XP
T8990	15562-15567	NNS	denotes	cells
T9007	15568-15570	IN	denotes	by
T9008	15571-15582	JJ	denotes	comparative
T9009	15583-15601	NN	denotes	immunofluorescence
T9010	15602-15604	IN	denotes	of
T9011	15605-15608	DT	denotes	the
T9013	15609-15612	NN	denotes	p62
T9012	15613-15620	NN	denotes	subunit
T9014	15621-15623	IN	denotes	of
T9015	15624-15629	NN	denotes	TFIIH
T9016	15629-15630	.	denotes	.
T9017	15630-16048	sentence	denotes	Roman numerals represent different microscopic slides and Arabic numerals different cell lines labelled as follows: (I) wt cells (1) labelled with 2-μm beads, XpdTTD/TTD cells (2) with 0.79-μm beads, and XpdTTD/KO cells (3) with no beads; (II) wt cells (1) labelled with 0.79-μm beads and XpdTTD/†XPCS cells (4) with no beads; and (III) wt cells (1) labelled with 0.79-μm beads and XpdTTD/†XP cells (5) with no beads.
T9018	15631-15636	JJ	denotes	Roman
T9019	15637-15645	NNS	denotes	numerals
T9020	15646-15655	VBP	denotes	represent
T9021	15656-15665	JJ	denotes	different
T9023	15666-15677	JJ	denotes	microscopic
T9022	15678-15684	NNS	denotes	slides
T9024	15685-15688	CC	denotes	and
T9025	15689-15695	JJ	denotes	Arabic
T9026	15696-15704	NNS	denotes	numerals
T9028	15705-15714	JJ	denotes	different
T9029	15715-15719	NN	denotes	cell
T9027	15720-15725	NNS	denotes	lines
T9030	15726-15734	VBN	denotes	labelled
T9031	15735-15737	IN	denotes	as
T9032	15738-15745	VBZ	denotes	follows
T9033	15745-15747	:	denotes	:
T9034	15747-15748	-LRB-	denotes	(
T9035	15748-15749	LS	denotes	I
T9037	15749-15750	-RRB-	denotes	)
T9038	15751-15753	NN	denotes	wt
T9036	15754-15759	NNS	denotes	cells
T9039	15760-15761	-LRB-	denotes	(
T9040	15761-15762	CD	denotes	1
T9041	15762-15763	-RRB-	denotes	)
T9042	15764-15772	VBN	denotes	labelled
T9043	15773-15777	IN	denotes	with
T9044	15778-15779	CD	denotes	2
T9046	15779-15780	HYPH	denotes	-
T9045	15780-15782	NN	denotes	μm
T9047	15783-15788	NNS	denotes	beads
T9048	15788-15790	,	denotes	,
T9049	15790-15796	NN	denotes	XpdTTD
T9051	15796-15797	HYPH	denotes	/
T9050	15797-15800	NN	denotes	TTD
T9052	15801-15806	NNS	denotes	cells
T9053	15807-15808	-LRB-	denotes	(
T9054	15808-15809	CD	denotes	2
T9055	15809-15810	-RRB-	denotes	)
T9056	15811-15815	IN	denotes	with
T9057	15816-15820	CD	denotes	0.79
T9059	15820-15821	HYPH	denotes	-
T9058	15821-15823	NN	denotes	μm
T9060	15824-15829	NNS	denotes	beads
T9061	15829-15831	,	denotes	,
T9062	15831-15834	CC	denotes	and
T9063	15835-15841	NN	denotes	XpdTTD
T9065	15841-15842	HYPH	denotes	/
T9064	15842-15844	NN	denotes	KO
T9066	15845-15850	NNS	denotes	cells
T9067	15851-15852	-LRB-	denotes	(
T9068	15852-15853	CD	denotes	3
T9069	15853-15854	-RRB-	denotes	)
T9070	15855-15859	IN	denotes	with
T9071	15860-15862	DT	denotes	no
T9072	15863-15868	NNS	denotes	beads
T9073	15868-15869	:	denotes	;
T9074	15870-15871	-LRB-	denotes	(
T9075	15871-15873	LS	denotes	II
T9077	15873-15874	-RRB-	denotes	)
T9078	15875-15877	NN	denotes	wt
T9076	15878-15883	NNS	denotes	cells
T9079	15884-15885	-LRB-	denotes	(
T9080	15885-15886	CD	denotes	1
T9081	15886-15887	-RRB-	denotes	)
T9082	15888-15896	VBN	denotes	labelled
T9083	15897-15901	IN	denotes	with
T9084	15902-15906	CD	denotes	0.79
T9086	15906-15907	HYPH	denotes	-
T9085	15907-15909	NN	denotes	μm
T9087	15910-15915	NNS	denotes	beads
T9088	15916-15919	CC	denotes	and
T9089	15920-15926	NN	denotes	XpdTTD
T9091	15926-15927	HYPH	denotes	/
T9090	15927-15932	NN	denotes	†XPCS
T9092	15933-15938	NNS	denotes	cells
T9093	15939-15940	-LRB-	denotes	(
T9094	15940-15941	CD	denotes	4
T9095	15941-15942	-RRB-	denotes	)
T9096	15943-15947	IN	denotes	with
T9097	15948-15950	DT	denotes	no
T9098	15951-15956	NNS	denotes	beads
T9099	15956-15957	:	denotes	;
T9100	15958-15961	CC	denotes	and
T9101	15962-15963	-LRB-	denotes	(
T9102	15963-15966	LS	denotes	III
T9104	15966-15967	-RRB-	denotes	)
T9105	15968-15970	NN	denotes	wt
T9103	15971-15976	NNS	denotes	cells
T9106	15977-15978	-LRB-	denotes	(
T9107	15978-15979	CD	denotes	1
T9108	15979-15980	-RRB-	denotes	)
T9109	15981-15989	VBN	denotes	labelled
T9110	15990-15994	IN	denotes	with
T9111	15995-15999	CD	denotes	0.79
T9113	15999-16000	HYPH	denotes	-
T9112	16000-16002	NN	denotes	μm
T9114	16003-16008	NNS	denotes	beads
T9115	16009-16012	CC	denotes	and
T9116	16013-16019	NN	denotes	XpdTTD
T9118	16019-16020	HYPH	denotes	/
T9117	16020-16023	NN	denotes	†XP
T9119	16024-16029	NNS	denotes	cells
T9120	16030-16031	-LRB-	denotes	(
T9121	16031-16032	CD	denotes	5
T9122	16032-16033	-RRB-	denotes	)
T9123	16034-16038	IN	denotes	with
T9124	16039-16041	DT	denotes	no
T9125	16042-16047	NNS	denotes	beads
T9126	16047-16048	.	denotes	.
T9127	16048-16167	sentence	denotes	(F) Quantification of immunofluorescent signal from at least 50 nuclei per cell line and 2–6 experiments per genotype.
T9128	16049-16050	-LRB-	denotes	(
T9129	16050-16051	LS	denotes	F
T9131	16051-16052	-RRB-	denotes	)
T9130	16053-16067	NN	denotes	Quantification
T9132	16068-16070	IN	denotes	of
T9133	16071-16088	JJ	denotes	immunofluorescent
T9134	16089-16095	NN	denotes	signal
T9135	16096-16100	IN	denotes	from
T9136	16101-16103	RB	denotes	at
T9138	16104-16109	RBS	denotes	least
T9137	16110-16112	CD	denotes	50
T9139	16113-16119	NNS	denotes	nuclei
T9140	16120-16123	IN	denotes	per
T9141	16124-16128	NN	denotes	cell
T9142	16129-16133	NN	denotes	line
T9143	16134-16137	CC	denotes	and
T9144	16138-16139	CD	denotes	2
T9146	16139-16140	SYM	denotes	–
T9145	16140-16141	CD	denotes	6
T9147	16142-16153	NNS	denotes	experiments
T9148	16154-16157	IN	denotes	per
T9149	16158-16166	NN	denotes	genotype
T9150	16166-16167	.	denotes	.
T9151	16167-16278	sentence	denotes	Bars representing cells analysed on the same microscopic slide are depicted side by side, with wt set at 100%.
T9152	16168-16172	NNS	denotes	Bars
T9154	16173-16185	VBG	denotes	representing
T9155	16186-16191	NNS	denotes	cells
T9156	16192-16200	VBN	denotes	analysed
T9157	16201-16203	IN	denotes	on
T9158	16204-16207	DT	denotes	the
T9160	16208-16212	JJ	denotes	same
T9161	16213-16224	JJ	denotes	microscopic
T9159	16225-16230	NN	denotes	slide
T9162	16231-16234	VBP	denotes	are
T9153	16235-16243	VBN	denotes	depicted
T9163	16244-16248	NN	denotes	side
T9164	16249-16251	IN	denotes	by
T9165	16252-16256	NN	denotes	side
T9166	16256-16258	,	denotes	,
T9167	16258-16262	IN	denotes	with
T9168	16263-16265	NN	denotes	wt
T9169	16266-16269	VBN	denotes	set
T9170	16270-16272	IN	denotes	at
T9171	16273-16276	CD	denotes	100
T9172	16276-16277	NN	denotes	%
T9173	16277-16278	.	denotes	.
T9174	16278-16433	sentence	denotes	The p-value indicates minimum significant difference between wt and the indicated cell lines analysed on the same microscopic slide within one experiment.
T9175	16279-16282	DT	denotes	The
T9177	16283-16284	NN	denotes	p
T9178	16284-16285	HYPH	denotes	-
T9176	16285-16290	NN	denotes	value
T9179	16291-16300	VBZ	denotes	indicates
T9180	16301-16308	JJ	denotes	minimum
T9182	16309-16320	JJ	denotes	significant
T9181	16321-16331	NN	denotes	difference
T9183	16332-16339	IN	denotes	between
T9184	16340-16342	NN	denotes	wt
T9185	16343-16346	CC	denotes	and
T9186	16347-16350	DT	denotes	the
T9188	16351-16360	VBN	denotes	indicated
T9189	16361-16365	NN	denotes	cell
T9187	16366-16371	NNS	denotes	lines
T9190	16372-16380	VBN	denotes	analysed
T9191	16381-16383	IN	denotes	on
T9192	16384-16387	DT	denotes	the
T9194	16388-16392	JJ	denotes	same
T9195	16393-16404	JJ	denotes	microscopic
T9193	16405-16410	NN	denotes	slide
T9196	16411-16417	IN	denotes	within
T9197	16418-16421	CD	denotes	one
T9198	16422-16432	NN	denotes	experiment
T9199	16432-16433	.	denotes	.
T3772	16434-16436	NN	denotes	Ne
T3771	16434-16443	sentence	denotes	Next we a
T3773	16436-16438	NNS	denotes	xt
T3774	16438-16439	:	denotes
T3775	16439-16441	VBD	denotes	we
T3776	16442-16443	DT	denotes	a
T3778	16443-16445	NN	denotes	sk
T3777	16443-16453	sentence	denotes	sked wheth
T3779	16445-16446	VBZ	denotes	e
T3781	16446-16447	NN	denotes	d
T3782	16448-16450	IN	denotes	wh
T3783	16450-16451	NNS	denotes	e
T3780	16451-16452	VBP	denotes	t
T3784	16452-16453	IN	denotes	h
T3786	16453-16455	NN	denotes	er
T3785	16453-16457	sentence	denotes	er t
T3787	16456-16457	NN	denotes	t
T3789	16457-16458	DT	denotes	h
T3788	16457-16459	sentence	denotes	he
T3790	16458-16459	NN	denotes	e
T3791	16459-16460	sentence	denotes
T3793	16460-16461	NN	denotes	X
T3792	16460-16463	sentence	denotes	Xpd
T3794	16461-16462	NN	denotes	p
T3795	16462-16463	NN	denotes	d
T3797	16463-16468	NN	denotes	†XPCS
T3796	16463-16482	sentence	denotes	†XPCS and Xpd†XP al
T3798	16469-16472	CC	denotes	and
T3799	16473-16476	NN	denotes	Xpd
T3800	16476-16479	NN	denotes	†XP
T3801	16480-16481	JJ	denotes	a
T3802	16481-16482	NN	denotes	l
T3804	16482-16483	NNS	denotes	l
T3803	16482-16500	sentence	denotes	leles, despite dec
T3805	16483-16484	VBP	denotes	e
T3806	16484-16487	NNS	denotes	les
T3807	16487-16489	,	denotes	,
T3808	16489-16490	NN	denotes	d
T3809	16490-16492	NNS	denotes	es
T3810	16492-16493	NN	denotes	p
T3811	16493-16495	IN	denotes	it
T3812	16495-16496	NNS	denotes	e
T3813	16497-16498	VBN	denotes	d
T3814	16498-16499	NNS	denotes	e
T3815	16499-16500	NNS	denotes	c
T3817	16500-16502	JJ	denotes	re
T3816	16500-16508	sentence	denotes	reased m
T3818	16502-16503	NN	denotes	a
T3819	16503-16504	RBS	denotes	s
T3820	16504-16505	NNS	denotes	e
T3821	16505-16506	CC	denotes	d
T3822	16507-16508	NNS	denotes	m
T3826	16508-16511	NN	denotes	RNA
T3823	16508-16641	sentence	denotes	RNA levels, ameliorated TTD symptoms by increasing overall TFIIH levels in compound heterozygous XpdTTD/ †XPCS and XpdTTD/ †XP cells.
T3825	16512-16518	NNS	denotes	levels
T3827	16518-16520	,	denotes	,
T3824	16520-16531	VBN	denotes	ameliorated
T3828	16532-16535	NN	denotes	TTD
T3829	16536-16544	NNS	denotes	symptoms
T3830	16545-16547	IN	denotes	by
T3831	16548-16558	VBG	denotes	increasing
T3833	16559-16566	JJ	denotes	overall
T3834	16567-16572	NN	denotes	TFIIH
T3832	16573-16579	NNS	denotes	levels
T3835	16580-16582	IN	denotes	in
T3836	16583-16591	NN	denotes	compound
T3838	16592-16604	JJ	denotes	heterozygous
T3839	16605-16611	NN	denotes	XpdTTD
T3841	16611-16612	HYPH	denotes	/
T3840	16612-16618	NN	denotes	†XPCS
T3842	16619-16622	CC	denotes	and
T3843	16623-16629	NN	denotes	XpdTTD
T3845	16629-16630	HYPH	denotes	/
T3844	16630-16634	NN	denotes	†XP
T3837	16635-16640	NNS	denotes	cells
T3846	16640-16641	.	denotes	.
T3847	16641-16863	sentence	denotes	Previously, using comparative immunohistochemistry, we and others have shown an up to 70% reduction of TFIIH levels in primary fibroblasts from patients with TTD compared with wt controls due to reduced stability [16,17].
T3848	16642-16652	RB	denotes	Previously
T3850	16652-16654	,	denotes	,
T3851	16654-16659	VBG	denotes	using
T3852	16660-16671	JJ	denotes	comparative
T3853	16672-16692	NN	denotes	immunohistochemistry
T3854	16692-16694	,	denotes	,
T3855	16694-16696	PRP	denotes	we
T3856	16697-16700	CC	denotes	and
T3857	16701-16707	NNS	denotes	others
T3858	16708-16712	VBP	denotes	have
T3849	16713-16718	VBN	denotes	shown
T3859	16719-16721	DT	denotes	an
T3861	16722-16724	IN	denotes	up
T3863	16725-16727	IN	denotes	to
T3862	16728-16730	CD	denotes	70
T3864	16730-16731	NN	denotes	%
T3860	16732-16741	NN	denotes	reduction
T3865	16742-16744	IN	denotes	of
T3866	16745-16750	NN	denotes	TFIIH
T3867	16751-16757	NNS	denotes	levels
T3868	16758-16760	IN	denotes	in
T3869	16761-16768	JJ	denotes	primary
T3870	16769-16780	NNS	denotes	fibroblasts
T3871	16781-16785	IN	denotes	from
T3872	16786-16794	NNS	denotes	patients
T3873	16795-16799	IN	denotes	with
T3874	16800-16803	NN	denotes	TTD
T3875	16804-16812	VBN	denotes	compared
T3876	16813-16817	IN	denotes	with
T3877	16818-16820	NN	denotes	wt
T3878	16821-16829	NNS	denotes	controls
T3879	16830-16833	IN	denotes	due
T3880	16834-16836	IN	denotes	to
T3881	16837-16844	VBN	denotes	reduced
T3882	16845-16854	NN	denotes	stability
T3883	16855-16856	-LRB-	denotes	[
T3885	16856-16858	CD	denotes	16
T3886	16858-16859	,	denotes	,
T3884	16859-16861	CD	denotes	17
T3887	16861-16862	-RRB-	denotes	]
T3888	16862-16863	.	denotes	.
T3889	16863-17117	sentence	denotes	Despite overexpression of mRNA from the XpdTTD allele relative to the wt allele (Figure 1E), TFIIH protein levels were reduced by 50% in primary mouse XpdTTD/TTD fibroblasts (Figure 4E and 4F), thereby mimicking the situation in human patients with TTD.
T3890	16864-16871	IN	denotes	Despite
T3892	16872-16886	NN	denotes	overexpression
T3893	16887-16889	IN	denotes	of
T3894	16890-16894	NN	denotes	mRNA
T3895	16895-16899	IN	denotes	from
T3896	16900-16903	DT	denotes	the
T3898	16904-16910	NN	denotes	XpdTTD
T3897	16911-16917	NN	denotes	allele
T3899	16918-16926	JJ	denotes	relative
T3900	16927-16929	IN	denotes	to
T3901	16930-16933	DT	denotes	the
T3903	16934-16936	NN	denotes	wt
T3902	16937-16943	NN	denotes	allele
T3904	16944-16945	-LRB-	denotes	(
T3906	16945-16951	NN	denotes	Figure
T3905	16952-16954	NN	denotes	1E
T3907	16954-16955	-RRB-	denotes	)
T3908	16955-16957	,	denotes	,
T3909	16957-16962	NN	denotes	TFIIH
T3911	16963-16970	NN	denotes	protein
T3910	16971-16977	NNS	denotes	levels
T3912	16978-16982	VBD	denotes	were
T3891	16983-16990	VBN	denotes	reduced
T3913	16991-16993	IN	denotes	by
T3914	16994-16996	CD	denotes	50
T3915	16996-16997	NN	denotes	%
T3916	16998-17000	IN	denotes	in
T3917	17001-17008	JJ	denotes	primary
T3919	17009-17014	NN	denotes	mouse
T3920	17015-17021	NN	denotes	XpdTTD
T3922	17021-17022	HYPH	denotes	/
T3921	17022-17025	NN	denotes	TTD
T3918	17026-17037	NNS	denotes	fibroblasts
T3923	17038-17039	-LRB-	denotes	(
T3925	17039-17045	NN	denotes	Figure
T3924	17046-17048	NN	denotes	4E
T3926	17049-17052	CC	denotes	and
T3927	17053-17055	NN	denotes	4F
T3928	17055-17056	-RRB-	denotes	)
T3929	17056-17058	,	denotes	,
T3930	17058-17065	RB	denotes	thereby
T3931	17066-17075	VBG	denotes	mimicking
T3932	17076-17079	DT	denotes	the
T3933	17080-17089	NN	denotes	situation
T3934	17090-17092	IN	denotes	in
T3935	17093-17098	JJ	denotes	human
T3936	17099-17107	NNS	denotes	patients
T3937	17108-17112	IN	denotes	with
T3938	17113-17116	NN	denotes	TTD
T3939	17116-17117	.	denotes	.
T3940	17117-17246	sentence	denotes	In accordance with the gene dosage, a further reduction of up to 70% of the wt level was observed in hemizygous XpdTTD/KO cells.
T3941	17118-17120	IN	denotes	In
T3943	17121-17131	NN	denotes	accordance
T3944	17132-17136	IN	denotes	with
T3945	17137-17140	DT	denotes	the
T3947	17141-17145	NN	denotes	gene
T3946	17146-17152	NN	denotes	dosage
T3948	17152-17154	,	denotes	,
T3949	17154-17155	DT	denotes	a
T3951	17156-17163	JJ	denotes	further
T3950	17164-17173	NN	denotes	reduction
T3952	17174-17176	IN	denotes	of
T3953	17177-17179	IN	denotes	up
T3955	17180-17182	IN	denotes	to
T3954	17183-17185	CD	denotes	70
T3956	17185-17186	NN	denotes	%
T3957	17187-17189	IN	denotes	of
T3958	17190-17193	DT	denotes	the
T3960	17194-17196	NN	denotes	wt
T3959	17197-17202	NN	denotes	level
T3961	17203-17206	VBD	denotes	was
T3942	17207-17215	VBN	denotes	observed
T3962	17216-17218	IN	denotes	in
T3963	17219-17229	JJ	denotes	hemizygous
T3965	17230-17236	NN	denotes	XpdTTD
T3967	17236-17237	HYPH	denotes	/
T3966	17237-17239	NN	denotes	KO
T3964	17240-17245	NNS	denotes	cells
T3968	17245-17246	.	denotes	.
T3969	17246-17440	sentence	denotes	Consistent with low mRNA expression levels, neither the Xpd†XPCS nor the Xpd†XP allele was able to restore TFIIH abundance to wt levels in XpdTTD compound heterozygote cells (Figure 4E and 4F).
T3970	17247-17257	JJ	denotes	Consistent
T3972	17258-17262	IN	denotes	with
T3973	17263-17266	JJ	denotes	low
T3975	17267-17271	NN	denotes	mRNA
T3976	17272-17282	NN	denotes	expression
T3974	17283-17289	NNS	denotes	levels
T3977	17289-17291	,	denotes	,
T3978	17291-17298	CC	denotes	neither
T3980	17299-17302	DT	denotes	the
T3981	17303-17311	NN	denotes	Xpd†XPCS
T3982	17312-17315	CC	denotes	nor
T3983	17316-17319	DT	denotes	the
T3984	17320-17326	NN	denotes	Xpd†XP
T3979	17327-17333	NN	denotes	allele
T3971	17334-17337	VBD	denotes	was
T3985	17338-17342	JJ	denotes	able
T3986	17343-17345	TO	denotes	to
T3987	17346-17353	VB	denotes	restore
T3988	17354-17359	NN	denotes	TFIIH
T3989	17360-17369	NN	denotes	abundance
T3990	17370-17372	IN	denotes	to
T3991	17373-17375	NN	denotes	wt
T3992	17376-17382	NNS	denotes	levels
T3993	17383-17385	IN	denotes	in
T3994	17386-17392	NN	denotes	XpdTTD
T3996	17393-17401	NN	denotes	compound
T3997	17402-17414	NN	denotes	heterozygote
T3995	17415-17420	NNS	denotes	cells
T3998	17421-17422	-LRB-	denotes	(
T4000	17422-17428	NN	denotes	Figure
T3999	17429-17431	NN	denotes	4E
T4001	17432-17435	CC	denotes	and
T4002	17436-17438	NN	denotes	4F
T4003	17438-17439	-RRB-	denotes	)
T4004	17439-17440	.	denotes	.
T4005	17440-17717	sentence	denotes	Thus, the improved UV survival observed in compound heterozygote cells (Figure 4A) and likely the rescue of TTD progeroid symptoms (Figure 3) were not due to normalisation of TFIIH levels, suggesting a qualitative rather than a quantitative effect on these phenotypes in vivo.
T4006	17441-17445	RB	denotes	Thus
T4008	17445-17447	,	denotes	,
T4009	17447-17450	DT	denotes	the
T4011	17451-17459	VBN	denotes	improved
T4012	17460-17462	NN	denotes	UV
T4010	17463-17471	NN	denotes	survival
T4013	17472-17480	VBN	denotes	observed
T4014	17481-17483	IN	denotes	in
T4015	17484-17492	NN	denotes	compound
T4017	17493-17505	NN	denotes	heterozygote
T4016	17506-17511	NNS	denotes	cells
T4018	17512-17513	-LRB-	denotes	(
T4020	17513-17519	NN	denotes	Figure
T4019	17520-17522	NN	denotes	4A
T4021	17522-17523	-RRB-	denotes	)
T4022	17524-17527	CC	denotes	and
T4023	17528-17534	RB	denotes	likely
T4025	17535-17538	DT	denotes	the
T4024	17539-17545	NN	denotes	rescue
T4026	17546-17548	IN	denotes	of
T4027	17549-17552	NN	denotes	TTD
T4029	17553-17562	NN	denotes	progeroid
T4028	17563-17571	NNS	denotes	symptoms
T4030	17572-17573	-LRB-	denotes	(
T4031	17573-17579	NN	denotes	Figure
T4032	17580-17581	CD	denotes	3
T4033	17581-17582	-RRB-	denotes	)
T4007	17583-17587	VBD	denotes	were
T4034	17588-17591	RB	denotes	not
T4035	17592-17595	IN	denotes	due
T4036	17596-17598	IN	denotes	to
T4037	17599-17612	NN	denotes	normalisation
T4038	17613-17615	IN	denotes	of
T4039	17616-17621	NN	denotes	TFIIH
T4040	17622-17628	NNS	denotes	levels
T4041	17628-17630	,	denotes	,
T4042	17630-17640	VBG	denotes	suggesting
T4043	17641-17642	DT	denotes	a
T4044	17643-17654	JJ	denotes	qualitative
T4046	17655-17661	RB	denotes	rather
T4047	17662-17666	IN	denotes	than
T4048	17667-17668	DT	denotes	a
T4049	17669-17681	JJ	denotes	quantitative
T4045	17682-17688	NN	denotes	effect
T4050	17689-17691	IN	denotes	on
T4051	17692-17697	DT	denotes	these
T4052	17698-17708	NNS	denotes	phenotypes
T4053	17709-17711	FW	denotes	in
T4054	17712-17716	FW	denotes	vivo
T4055	17716-17717	.	denotes	.
T4056	17717-17866	sentence	denotes	In contrast, the level of XPCS mRNA expression did affect the ability of the encoded protein (XPDG602D) to restore the TTD hair phenotype to normal.
T4057	17718-17720	IN	denotes	In
T4059	17721-17729	NN	denotes	contrast
T4060	17729-17731	,	denotes	,
T4061	17731-17734	DT	denotes	the
T4062	17735-17740	NN	denotes	level
T4063	17741-17743	IN	denotes	of
T4064	17744-17748	NN	denotes	XPCS
T4066	17749-17753	NN	denotes	mRNA
T4065	17754-17764	NN	denotes	expression
T4067	17765-17768	VBD	denotes	did
T4058	17769-17775	VB	denotes	affect
T4068	17776-17779	DT	denotes	the
T4069	17780-17787	NN	denotes	ability
T4070	17788-17790	IN	denotes	of
T4071	17791-17794	DT	denotes	the
T4073	17795-17802	VBN	denotes	encoded
T4072	17803-17810	NN	denotes	protein
T4074	17811-17812	-LRB-	denotes	(
T4075	17812-17820	NN	denotes	XPDG602D
T4076	17820-17821	-RRB-	denotes	)
T4077	17822-17824	TO	denotes	to
T4078	17825-17832	VB	denotes	restore
T4079	17833-17836	DT	denotes	the
T4081	17837-17840	NN	denotes	TTD
T4082	17841-17845	NN	denotes	hair
T4080	17846-17855	NN	denotes	phenotype
T4083	17856-17858	IN	denotes	to
T4084	17859-17865	JJ	denotes	normal
T4085	17865-17866	.	denotes	.
T4086	17866-18129	sentence	denotes	Notably, XpdTTD/ †XPCS animals had a partial TTD hair phenotype, correlating with low levels of Xpd†XPCS expression, whereas XpdTTD/XPCS animals had wt hair, correlating with normal expression levels from the viable XpdXPCS allele (Table 2 and unpublished data).
T4087	17867-17874	RB	denotes	Notably
T4089	17874-17876	,	denotes	,
T4090	17876-17882	NN	denotes	XpdTTD
T4092	17882-17883	HYPH	denotes	/
T4091	17883-17889	NN	denotes	†XPCS
T4093	17890-17897	NNS	denotes	animals
T4088	17898-17901	VBD	denotes	had
T4094	17902-17903	DT	denotes	a
T4096	17904-17911	JJ	denotes	partial
T4097	17912-17915	NN	denotes	TTD
T4098	17916-17920	NN	denotes	hair
T4095	17921-17930	NN	denotes	phenotype
T4099	17930-17932	,	denotes	,
T4100	17932-17943	VBG	denotes	correlating
T4101	17944-17948	IN	denotes	with
T4102	17949-17952	JJ	denotes	low
T4103	17953-17959	NNS	denotes	levels
T4104	17960-17962	IN	denotes	of
T4105	17963-17971	NN	denotes	Xpd†XPCS
T4106	17972-17982	NN	denotes	expression
T4107	17982-17984	,	denotes	,
T4108	17984-17991	IN	denotes	whereas
T4110	17992-17998	NN	denotes	XpdTTD
T4112	17998-17999	HYPH	denotes	/
T4111	17999-18003	NN	denotes	XPCS
T4113	18004-18011	NNS	denotes	animals
T4109	18012-18015	VBD	denotes	had
T4114	18016-18018	NN	denotes	wt
T4115	18019-18023	NN	denotes	hair
T4116	18023-18025	,	denotes	,
T4117	18025-18036	VBG	denotes	correlating
T4118	18037-18041	IN	denotes	with
T4119	18042-18048	JJ	denotes	normal
T4121	18049-18059	NN	denotes	expression
T4120	18060-18066	NNS	denotes	levels
T4122	18067-18071	IN	denotes	from
T4123	18072-18075	DT	denotes	the
T4125	18076-18082	JJ	denotes	viable
T4126	18083-18090	NN	denotes	XpdXPCS
T4124	18091-18097	NN	denotes	allele
T4127	18098-18099	-LRB-	denotes	(
T4128	18099-18104	NN	denotes	Table
T4129	18105-18106	CD	denotes	2
T4130	18107-18110	CC	denotes	and
T4131	18111-18122	JJ	denotes	unpublished
T4132	18123-18127	NNS	denotes	data
T4133	18127-18128	-RRB-	denotes	)
T4134	18128-18129	.	denotes	.
T4135	18129-18257	sentence	denotes	Thus, the range of expression levels from these two mutant alleles affected their ability to complement some phenotypes (hair).
T4136	18130-18134	RB	denotes	Thus
T4138	18134-18136	,	denotes	,
T4139	18136-18139	DT	denotes	the
T4140	18140-18145	NN	denotes	range
T4141	18146-18148	IN	denotes	of
T4142	18149-18159	NN	denotes	expression
T4143	18160-18166	NNS	denotes	levels
T4144	18167-18171	IN	denotes	from
T4145	18172-18177	DT	denotes	these
T4147	18178-18181	CD	denotes	two
T4148	18182-18188	JJ	denotes	mutant
T4146	18189-18196	NNS	denotes	alleles
T4137	18197-18205	VBD	denotes	affected
T4149	18206-18211	PRP$	denotes	their
T4150	18212-18219	NN	denotes	ability
T4151	18220-18222	TO	denotes	to
T4152	18223-18233	VB	denotes	complement
T4153	18234-18238	DT	denotes	some
T4154	18239-18249	NNS	denotes	phenotypes
T4155	18250-18251	-LRB-	denotes	(
T4156	18251-18255	NN	denotes	hair
T4157	18255-18256	-RRB-	denotes	)
T4158	18256-18257	.	denotes	.
T4159	18257-18420	sentence	denotes	An overview of the functional relationships between Xpd alleles, phenotypes, and the presumed underlying TFIIH function in mice and cells is presented in Table 2.
T4160	18258-18260	DT	denotes	An
T4161	18261-18269	NN	denotes	overview
T4163	18270-18272	IN	denotes	of
T4164	18273-18276	DT	denotes	the
T4166	18277-18287	JJ	denotes	functional
T4165	18288-18301	NNS	denotes	relationships
T4167	18302-18309	IN	denotes	between
T4168	18310-18313	NN	denotes	Xpd
T4169	18314-18321	NNS	denotes	alleles
T4170	18321-18323	,	denotes	,
T4171	18323-18333	NNS	denotes	phenotypes
T4172	18333-18335	,	denotes	,
T4173	18335-18338	CC	denotes	and
T4174	18339-18342	DT	denotes	the
T4176	18343-18351	VBN	denotes	presumed
T4177	18352-18362	VBG	denotes	underlying
T4178	18363-18368	NN	denotes	TFIIH
T4175	18369-18377	NN	denotes	function
T4179	18378-18380	IN	denotes	in
T4180	18381-18385	NNS	denotes	mice
T4181	18386-18389	CC	denotes	and
T4182	18390-18395	NNS	denotes	cells
T4183	18396-18398	VBZ	denotes	is
T4162	18399-18408	VBN	denotes	presented
T4184	18409-18411	IN	denotes	in
T4185	18412-18417	NN	denotes	Table
T4186	18418-18419	CD	denotes	2
T4187	18419-18420	.	denotes	.
R1000	T1496	T1497	mark	Because,was
R1001	T1497	T1500	advcl	was,expected
R1002	T1498	T1499	compound	patient,XPCS2
R1003	T1499	T1497	nsubj	XPCS2,was
R1004	T1501	T1502	det	a,hemizygote
R1005	T1502	T1497	attr	hemizygote,was
R1006	T1503	T1502	prep	with,hemizygote
R1007	T1504	T1505	amod	mutant,protein
R1008	T1505	T1503	pobj	protein,with
R1009	T1506	T1505	compound	XPD,protein
R1010	T1507	T1508	punct	(,XPDG602D
R1011	T1508	T1505	parataxis	XPDG602D,protein
R1012	T1509	T1508	punct	),XPDG602D
R1013	T1510	T1505	acl	expressed,protein
R1014	T1511	T1510	prep	from,expressed
R1015	T1512	T1513	det	a,allele
R1016	T1513	T1511	pobj	allele,from
R1017	T1514	T1513	amod	single,allele
R1018	T1515	T1500	punct	", ",expected
R1019	T1516	T1517	det	the,mutation
R1020	T1517	T1500	nsubjpass	mutation,expected
R1021	T1518	T1517	amod	corresponding,mutation
R1022	T1519	T1500	auxpass	was,expected
R1023	T1520	T1521	aux	to,be
R1024	T1521	T1500	xcomp	be,expected
R1025	T1522	T1521	acomp	viable,be
R1026	T1523	T1521	prep	in,be
R1027	T1524	T1525	det	the,state
R1028	T1525	T1523	pobj	state,in
R1029	T1526	T1525	amod	homozygous,state
R1030	T1527	T1500	punct	.,expected
R1031	T1529	T1530	advmod	However,observed
R1032	T1531	T1530	punct	", ",observed
R1033	T1532	T1533	amod	homozygous,mice
R1034	T1533	T1530	nsubjpass	mice,observed
R1035	T1534	T1533	amod	mutant,mice
R1036	T1535	T1530	auxpass	were,observed
R1037	T1536	T1530	neg	not,observed
R1038	T1537	T1530	punct	", ",observed
R1039	T1538	T1539	preconj	neither,amongst
R1040	T1539	T1530	prep	amongst,observed
R1041	T1540	T1541	amod	live,births
R1042	T1541	T1539	pobj	births,amongst
R1043	T1542	T1541	cc	nor,births
R1044	T1543	T1544	amod	embryonic,day
R1045	T1544	T1545	nmod	day,embryos
R1046	T1545	T1541	conj	embryos,births
R1047	T1546	T1544	nummod	13.5,day
R1048	T1547	T1544	punct	(,day
R1049	T1548	T1544	appos	E13.5,day
R1050	T1549	T1544	punct	),day
R1051	T1550	T1544	cc	or,day
R1052	T1551	T1544	conj	E3.5,day
R1053	T1552	T1553	punct	(,Table
R1054	T1553	T1530	parataxis	Table,observed
R1055	T1554	T1553	nummod	1,Table
R1056	T1555	T1553	punct	),Table
R1057	T1556	T1530	punct	.,observed
R1058	T1558	T1559	det	The,allele
R1059	T1559	T1564	nsubjpass	allele,designated
R1060	T1560	T1559	amod	corresponding,allele
R1061	T1561	T1559	amod	hypomorphic,allele
R1062	T1562	T1559	punct	", ",allele
R1063	T1563	T1559	amod	mutant,allele
R1064	T1565	T1564	auxpass	was,designated
R1065	T1566	T1564	advmod	thus,designated
R1066	T1567	T1564	prep	as,designated
R1067	T1568	T1569	amod	homozygous,lethal
R1068	T1569	T1567	pobj	lethal,as
R1069	T1570	T1571	punct	(,†XPCS
R1070	T1571	T1569	parataxis	†XPCS,lethal
R1071	T1572	T1571	punct	),†XPCS
R1072	T1573	T1564	punct	.,designated
R1073	T1575	T1576	amod	Homozygous,lethality
R1074	T1576	T1577	nsubj	lethality,is
R1075	T1578	T1576	prep	of,lethality
R1076	T1579	T1580	det	the,allele
R1077	T1580	T1578	pobj	allele,of
R1078	T1581	T1580	compound	XPCS,allele
R1079	T1582	T1583	advmod	likely,due
R1080	T1583	T1577	prep	due,is
R1081	T1584	T1583	prep	to,due
R1082	T1585	T1586	amod	reduced,levels
R1083	T1586	T1584	pobj	levels,to
R1084	T1587	T1586	prep	of,levels
R1085	T1588	T1587	pobj	expression,of
R1086	T1589	T1588	prep	of,expression
R1087	T1590	T1591	det	this,protein
R1088	T1591	T1589	pobj	protein,of
R1089	T1592	T1591	amod	essential,protein
R1090	T1593	T1586	prep	as,levels
R1091	T1594	T1595	det	a,result
R1092	T1595	T1593	pobj	result,as
R1093	T1596	T1595	prep	of,result
R1094	T1597	T1598	compound	gene,targeting
R1095	T1598	T1596	pobj	targeting,of
R1096	T1599	T1600	punct	(,1A
R1097	T1600	T1598	parataxis	1A,targeting
R1098	T1601	T1600	compound	Figure,1A
R1099	T1602	T1600	punct	),1A
R1100	T1603	T1604	amod	rather,than
R1101	T1604	T1584	cc	than,to
R1102	T1605	T1584	conj	to,to
R1103	T1606	T1607	det	the,mutation
R1104	T1607	T1605	pobj	mutation,to
R1105	T1608	T1607	appos	itself,mutation
R1106	T1609	T1577	punct	.,is
R1107	T1611	T1612	compound	Xpd,ablation
R1108	T1612	T1613	nsubj	ablation,is
R1109	T1614	T1615	punct	(,KO
R1110	T1615	T1612	parataxis	KO,ablation
R1111	T1616	T1615	compound	XpdKO,KO
R1112	T1617	T1615	punct	/,KO
R1113	T1618	T1615	punct	),KO
R1114	T1619	T1620	advmod	similarly,incompatible
R1115	T1620	T1613	acomp	incompatible,is
R1116	T1621	T1620	prep	with,incompatible
R1117	T1622	T1621	pobj	life,with
R1118	T1623	T1622	prep	beyond,life
R1119	T1624	T1625	det	the,stages
R1120	T1625	T1623	pobj	stages,beyond
R1121	T1626	T1625	amod	earliest,stages
R1122	T1627	T1625	prep	of,stages
R1123	T1628	T1627	pobj	embryogenesis,of
R1124	T1629	T1630	punct	[,22
R1125	T1630	T1613	parataxis	22,is
R1126	T1631	T1630	punct	],22
R1127	T1632	T1613	punct	.,is
R1128	T1634	T1635	advcl	Consistent,was
R1129	T1636	T1634	prep	with,Consistent
R1130	T1637	T1638	det	this,interpretation
R1131	T1638	T1636	pobj	interpretation,with
R1132	T1639	T1635	punct	", ",was
R1133	T1640	T1641	det	a,mutation
R1134	T1641	T1635	nsubj	mutation,was
R1135	T1642	T1641	amod	different,mutation
R1136	T1643	T1641	amod	targeted,mutation
R1137	T1644	T1641	compound	Xpd,mutation
R1138	T1645	T1641	acl	encoding,mutation
R1139	T1646	T1645	dobj	XPDR683W,encoding
R1140	T1647	T1646	punct	", ",XPDR683W
R1141	T1648	T1649	dep	which,associated
R1142	T1649	T1646	relcl	associated,XPDR683W
R1143	T1650	T1649	auxpass	is,associated
R1144	T1651	T1649	prep	with,associated
R1145	T1652	T1651	pobj	XP,with
R1146	T1653	T1649	prep	in,associated
R1147	T1654	T1655	det	the,state
R1148	T1655	T1653	pobj	state,in
R1149	T1656	T1655	amod	homozygous,state
R1150	T1657	T1649	prep	in,associated
R1151	T1658	T1657	pobj	humans,in
R1152	T1659	T1635	punct	", ",was
R1153	T1660	T1661	advmod	similarly,underexpressed
R1154	T1661	T1635	acomp	underexpressed,was
R1155	T1662	T1661	cc	and,underexpressed
R1156	T1663	T1661	conj	lethal,underexpressed
R1157	T1664	T1635	prep	in,was
R1158	T1665	T1666	det	the,state
R1159	T1666	T1664	pobj	state,in
R1160	T1667	T1666	amod	homozygous,state
R1161	T1668	T1669	punct	(,designated
R1162	T1669	T1635	parataxis	designated,was
R1163	T1670	T1669	prep	as,designated
R1164	T1671	T1672	compound	†XP,allele
R1165	T1672	T1670	pobj	allele,as
R1166	T1673	T1669	punct	),designated
R1167	T1674	T1675	punct	(,1A
R1168	T1675	T1635	parataxis	1A,was
R1169	T1676	T1675	compound	Figure,1A
R1170	T1677	T1678	punct	–,1C
R1171	T1678	T1675	prep	1C,1A
R1172	T1679	T1675	punct	;,1A
R1173	T1680	T1675	appos	Table,1A
R1174	T1681	T1680	nummod	1,Table
R1175	T1682	T1675	punct	;,1A
R1176	T1683	T1684	amod	unpublished,data
R1177	T1684	T1675	appos	data,1A
R1178	T1685	T1675	punct	),1A
R1179	T1686	T1635	punct	.,was
R1180	T1688	T1689	advmod	Also,resulted
R1181	T1690	T1689	punct	", ",resulted
R1182	T1691	T1692	det	a,approach
R1183	T1692	T1689	nsubj	approach,resulted
R1184	T1693	T1692	amod	different,approach
R1185	T1694	T1692	compound	targeting,approach
R1186	T1695	T1692	acl	leading,approach
R1187	T1696	T1695	prep	to,leading
R1188	T1697	T1698	det	the,use
R1189	T1698	T1696	pobj	use,to
R1190	T1699	T1698	prep	of,use
R1191	T1700	T1701	det	the,UTR
R1192	T1701	T1699	pobj	UTR,of
R1193	T1702	T1701	amod	native,UTR
R1194	T1703	T1701	nummod	3,UTR
R1195	T1704	T1703	punct	′,3
R1196	T1705	T1698	cc	and,use
R1197	T1706	T1698	conj	removal,use
R1198	T1707	T1706	prep	of,removal
R1199	T1708	T1709	det	the,gene
R1200	T1709	T1707	pobj	gene,of
R1201	T1710	T1709	compound	neo,gene
R1202	T1711	T1689	prep	in,resulted
R1203	T1712	T1711	pobj	normalisation,in
R1204	T1713	T1712	prep	of,normalisation
R1205	T1714	T1715	compound	XpdXPCS,levels
R1206	T1715	T1713	pobj	levels,of
R1207	T1716	T1715	compound	mRNA,levels
R1208	T1717	T1712	cc	and,normalisation
R1209	T1718	T1719	amod	viable,animals
R1210	T1719	T1712	conj	animals,normalisation
R1211	T1720	T1719	amod	homozygous,animals
R1212	T1721	T1722	nmod	XpdXPCS,XPCS
R1213	T1722	T1719	nmod	XPCS,animals
R1214	T1723	T1722	punct	/,XPCS
R1215	T1724	T1725	punct	(,G602D
R1216	T1725	T1722	parataxis	G602D,XPCS
R1217	T1726	T1725	compound	XPDG602D,G602D
R1218	T1727	T1725	punct	/,G602D
R1219	T1728	T1725	punct	),G602D
R1220	T1729	T1730	punct	[,23
R1221	T1730	T1689	parataxis	23,resulted
R1222	T1731	T1730	punct	],23
R1223	T1732	T1689	punct	.,resulted
R1226	T1954	T1955	punct	“,Allele
R1227	T1955	T1958	nsubj	Allele,Alleviate
R1228	T1956	T1955	amod	Null,Allele
R1229	T1957	T1955	punct	”,Allele
R1230	T1959	T1958	aux	Can,Alleviate
R1231	T1960	T1961	amod	Developmental,Delay
R1232	T1961	T1958	dobj	Delay,Alleviate
R1233	T1962	T1961	punct	", ",Delay
R1234	T1963	T1964	nmod	Skin,Features
R1235	T1964	T1961	appos	Features,Delay
R1236	T1965	T1963	punct	", ",Skin
R1237	T1966	T1963	cc	and,Skin
R1238	T1967	T1963	conj	Hair,Skin
R1239	T1968	T1964	prep	of,Features
R1240	T1969	T1968	pobj	TTD,of
R1241	T1971	T1972	aux	To,test
R1242	T1972	T1973	advcl	test,combined
R1243	T1974	T1975	det	the,potential
R1244	T1975	T1972	dobj	potential,test
R1245	T1976	T1975	prep	of,potential
R1246	T1977	T1978	det	a,allele
R1247	T1978	T1976	pobj	allele,of
R1248	T1979	T1978	amod	homozygous,allele
R1249	T1980	T1978	amod	lethal,allele
R1250	T1981	T1978	punct	“,allele
R1251	T1982	T1978	amod	null,allele
R1252	T1983	T1978	punct	”,allele
R1253	T1984	T1985	aux	to,contribute
R1254	T1985	T1975	acl	contribute,potential
R1255	T1986	T1985	advmod	nevertheless,contribute
R1256	T1987	T1985	prep	to,contribute
R1257	T1988	T1989	amod	organismal,phenotype
R1258	T1989	T1987	pobj	phenotype,to
R1259	T1990	T1973	punct	", ",combined
R1260	T1991	T1973	nsubj	we,combined
R1261	T1992	T1993	det	an,allele
R1262	T1993	T1973	dobj	allele,combined
R1263	T1994	T1993	compound	Xpd†XPCS,allele
R1264	T1995	T1993	prep	with,allele
R1265	T1996	T1997	det	a,allele
R1266	T1997	T1995	pobj	allele,with
R1267	T1998	T1997	amod	viable,allele
R1268	T1999	T1997	compound	XpdTTD,allele
R1269	T2000	T1973	prep	by,combined
R1270	T2001	T2000	pcomp	crossing,by
R1271	T2002	T2003	det	the,animals
R1272	T2003	T2001	dobj	animals,crossing
R1273	T2004	T2003	amod	corresponding,animals
R1274	T2005	T2003	amod	heterozygous,animals
R1275	T2006	T1973	punct	.,combined
R1276	T2008	T2009	advcl	Similar,born
R1277	T2010	T2008	prep	to,Similar
R1278	T2011	T2012	amod	hemizygous,mice
R1279	T2012	T2010	pobj	mice,to
R1280	T2013	T2012	compound	TTD,mice
R1281	T2014	T2012	acl	carrying,mice
R1282	T2015	T2016	nummod	one,allele
R1283	T2016	T2014	dobj	allele,carrying
R1284	T2017	T2016	amod	true,allele
R1285	T2018	T2019	compound	Xpd,knockout
R1286	T2019	T2016	compound	knockout,allele
R1287	T2020	T2021	punct	(,KO
R1288	T2021	T2016	parataxis	KO,allele
R1289	T2022	T2021	compound	XpdTTD,KO
R1290	T2023	T2021	punct	/,KO
R1291	T2024	T2021	punct	),KO
R1292	T2025	T2009	punct	", ",born
R1293	T2026	T2027	nmod	compound,mice
R1294	T2027	T2009	nsubjpass	mice,born
R1295	T2028	T2027	amod	heterozygous,mice
R1296	T2029	T2030	compound	XpdTTD,†XPCS
R1297	T2030	T2027	compound	†XPCS,mice
R1298	T2031	T2030	punct	/,†XPCS
R1299	T2032	T2009	auxpass	were,born
R1300	T2033	T2009	prep	at,born
R1301	T2034	T2035	det	the,frequencies
R1302	T2035	T2033	pobj	frequencies,at
R1303	T2036	T2035	amod	expected,frequencies
R1304	T2037	T2035	amod	Mendelian,frequencies
R1305	T2038	T2009	punct	.,born
R1306	T2040	T2041	nsubjpass	Expression,reduced
R1307	T2042	T2040	prep	from,Expression
R1308	T2043	T2044	det	the,allele
R1309	T2044	T2042	pobj	allele,from
R1310	T2045	T2044	compound	Xpd†XPCS,allele
R1311	T2046	T2041	auxpass	was,reduced
R1312	T2047	T2041	advmod	also,reduced
R1313	T2048	T2041	prep	in,reduced
R1314	T2049	T2050	det	the,testis
R1315	T2050	T2048	pobj	testis,in
R1316	T2051	T2050	prep	of,testis
R1317	T2052	T2053	nmod	compound,animals
R1318	T2053	T2051	pobj	animals,of
R1319	T2054	T2053	amod	heterozygous,animals
R1320	T2055	T2041	punct	", ",reduced
R1321	T2056	T2057	mark	whereas,increased
R1322	T2057	T2041	advcl	increased,reduced
R1323	T2058	T2057	nsubjpass	expression,increased
R1324	T2059	T2058	prep	from,expression
R1325	T2060	T2061	det	the,allele
R1326	T2061	T2059	pobj	allele,from
R1327	T2062	T2061	compound	XpdTTD,allele
R1328	T2063	T2057	auxpass	was,increased
R1329	T2064	T2057	advcl	relative,increased
R1330	T2065	T2064	prep	to,relative
R1331	T2066	T2065	pobj	wt,to
R1332	T2067	T2057	prep	by,increased
R1333	T2068	T2069	punct	~,5-fold
R1334	T2069	T2067	pobj	5-fold,by
R1335	T2070	T2071	punct	(,1E
R1336	T2071	T2057	parataxis	1E,increased
R1337	T2072	T2071	compound	Figure,1E
R1338	T2073	T2071	punct	),1E
R1339	T2074	T2041	punct	.,reduced
R1340	T2076	T2077	prep	Because,were
R1341	T2078	T2076	pcomp	of,Because
R1342	T2079	T2080	det	a,lack
R1343	T2080	T2076	pobj	lack,Because
R1344	T2081	T2080	prep	of,lack
R1345	T2082	T2083	amod	available,antibodies
R1346	T2083	T2081	pobj	antibodies,of
R1347	T2084	T2083	cc	and,antibodies
R1348	T2085	T2086	det	the,inability
R1349	T2086	T2083	conj	inability,antibodies
R1350	T2087	T2088	aux	to,distinguish
R1351	T2088	T2086	acl	distinguish,inability
R1352	T2089	T2088	prep	amongst,distinguish
R1353	T2090	T2091	amod	various,forms
R1354	T2091	T2089	pobj	forms,amongst
R1355	T2092	T2091	amod	mutant,forms
R1356	T2093	T2091	prep	of,forms
R1357	T2094	T2093	pobj	XPD,of
R1358	T2095	T2091	acl	differing,forms
R1359	T2096	T2095	advmod	only,differing
R1360	T2097	T2095	prep	by,differing
R1361	T2098	T2099	amod	single,substitutions
R1362	T2099	T2097	pobj	substitutions,by
R1363	T2100	T2101	compound	amino,acid
R1364	T2101	T2099	compound	acid,substitutions
R1365	T2102	T2077	punct	", ",were
R1366	T2103	T2077	nsubj	we,were
R1367	T2104	T2077	acomp	unable,were
R1368	T2105	T2106	aux	to,ascertain
R1369	T2106	T2104	xcomp	ascertain,unable
R1370	T2107	T2108	det	the,amount
R1371	T2108	T2106	dobj	amount,ascertain
R1372	T2109	T2108	amod	relative,amount
R1373	T2110	T2108	prep	of,amount
R1374	T2111	T2112	compound	XPD,protein
R1375	T2112	T2110	pobj	protein,of
R1376	T2113	T2112	prep	from,protein
R1377	T2114	T2115	det	the,alleles
R1378	T2115	T2113	pobj	alleles,from
R1379	T2116	T2115	amod	different,alleles
R1380	T2117	T2077	punct	.,were
R1381	T2119	T2120	prep	Despite,ameliorated
R1382	T2121	T2122	amod	reduced,levels
R1383	T2122	T2119	pobj	levels,Despite
R1384	T2123	T2122	prep	of,levels
R1385	T2124	T2125	compound	mRNA,expression
R1386	T2125	T2123	pobj	expression,of
R1387	T2126	T2120	punct	", ",ameliorated
R1388	T2127	T2128	det	the,allele
R1389	T2128	T2120	nsubj	allele,ameliorated
R1390	T2129	T2128	amod	homozygous,allele
R1391	T2130	T2128	amod	lethal,allele
R1392	T2131	T2128	compound	Xpd†XPCS,allele
R1393	T2132	T2133	amod	multiple,symptoms
R1394	T2133	T2120	dobj	symptoms,ameliorated
R1395	T2134	T2135	npadvmod	XpdTTD,associated
R1396	T2135	T2133	amod	associated,symptoms
R1397	T2136	T2135	punct	-,associated
R1398	T2137	T2133	compound	disease,symptoms
R1399	T2138	T2120	prep	in,ameliorated
R1400	T2139	T2140	amod	compound,animals
R1401	T2140	T2138	pobj	animals,in
R1402	T2141	T2140	amod	heterozygous,animals
R1403	T2142	T2143	compound	XpdTTD,†XPCS
R1404	T2143	T2140	compound	†XPCS,animals
R1405	T2144	T2143	punct	/,†XPCS
R1406	T2145	T2120	prep	including,ameliorated
R1407	T2146	T2147	det	the,hair
R1408	T2147	T2145	pobj	hair,including
R1409	T2148	T2147	nmod	hallmark,hair
R1410	T2149	T2147	amod	brittle,hair
R1411	T2150	T2147	cc	and,hair
R1412	T2151	T2152	amod	cutaneous,features
R1413	T2152	T2147	conj	features,hair
R1414	T2153	T2154	advmod	fully,penetrant
R1415	T2154	T2147	amod	penetrant,hair
R1416	T2155	T2154	prep	in,penetrant
R1417	T2156	T2157	advmod	homo,hemizygous
R1418	T2157	T2160	amod	hemizygous,mice
R1419	T2158	T2157	punct	-,hemizygous
R1420	T2159	T2157	cc	and,hemizygous
R1421	T2160	T2155	pobj	mice,in
R1422	T2161	T2160	compound	TTD,mice
R1423	T2162	T2163	punct	(,2A
R1424	T2163	T2120	parataxis	2A,ameliorated
R1425	T2164	T2163	compound	Figure,2A
R1426	T2165	T2166	punct	–,2C
R1427	T2166	T2163	prep	2C,2A
R1428	T2167	T2163	punct	),2A
R1429	T2168	T2120	punct	.,ameliorated
R1430	T2170	T2171	prep	In,displayed
R1431	T2172	T2173	amod	marked,contrast
R1432	T2173	T2170	pobj	contrast,In
R1433	T2174	T2173	prep	to,contrast
R1434	T2175	T2176	nmod	XpdTTD,TTD
R1435	T2176	T2178	nmod	TTD,mice
R1436	T2177	T2176	punct	/,TTD
R1437	T2178	T2174	pobj	mice,to
R1438	T2179	T2176	punct	(,TTD
R1439	T2180	T2176	cc	and,TTD
R1440	T2181	T2182	compound	XpdTTD,KO
R1441	T2182	T2176	conj	KO,TTD
R1442	T2183	T2182	punct	/,KO
R1443	T2184	T2178	punct	),mice
R1444	T2185	T2178	punct	", ",mice
R1445	T2186	T2187	dep	which,display
R1446	T2187	T2178	relcl	display,mice
R1447	T2188	T2189	amod	complete,loss
R1448	T2189	T2187	dobj	loss,display
R1449	T2190	T2189	compound	hair,loss
R1450	T2191	T2189	prep	in,loss
R1451	T2192	T2193	det	the,cycle
R1452	T2193	T2191	pobj	cycle,in
R1453	T2194	T2193	amod	first,cycle
R1454	T2195	T2193	compound	hair,cycle
R1455	T2196	T2189	cc	and,loss
R1456	T2197	T2198	amod	partial,loss
R1457	T2198	T2189	conj	loss,loss
R1458	T2199	T2198	compound	hair,loss
R1459	T2200	T2198	prep	in,loss
R1460	T2201	T2202	amod	subsequent,cycles
R1461	T2202	T2200	pobj	cycles,in
R1462	T2203	T2187	prep	throughout,display
R1463	T2204	T2205	poss	their,lives
R1464	T2205	T2203	pobj	lives,throughout
R1465	T2206	T2207	punct	[,21
R1466	T2207	T2187	parataxis	21,display
R1467	T2208	T2207	punct	],21
R1468	T2209	T2171	punct	", ",displayed
R1469	T2210	T2211	nmod	compound,mice
R1470	T2211	T2171	nsubj	mice,displayed
R1471	T2212	T2211	amod	heterozygous,mice
R1472	T2213	T2211	compound	XpdTTD/†XPCS,mice
R1473	T2214	T2215	det	some,loss
R1474	T2215	T2171	dobj	loss,displayed
R1475	T2216	T2215	compound	hair,loss
R1476	T2217	T2218	advmod	only,during
R1477	T2218	T2171	prep	during,displayed
R1478	T2219	T2220	det	the,cycle
R1479	T2220	T2218	pobj	cycle,during
R1480	T2221	T2220	amod	first,cycle
R1481	T2222	T2220	compound	hair,cycle
R1482	T2223	T2218	cc	and,during
R1483	T2224	T2225	advmod	only,locally
R1484	T2225	T2226	advmod	locally,at
R1485	T2226	T2218	conj	at,during
R1486	T2227	T2228	det	the,back
R1487	T2228	T2226	pobj	back,at
R1488	T2229	T2230	punct	(,2A
R1489	T2230	T2171	parataxis	2A,displayed
R1490	T2231	T2230	compound	Figure,2A
R1491	T2232	T2230	punct	),2A
R1492	T2233	T2171	punct	.,displayed
R1493	T2235	T2236	compound	Scanning,microscope
R1494	T2236	T2238	compound	microscope,analysis
R1495	T2237	T2236	compound	electron,microscope
R1496	T2238	T2239	nsubj	analysis,revealed
R1497	T2240	T2238	prep	of,analysis
R1498	T2241	T2242	compound	XpdTTD,†XPCS
R1499	T2242	T2244	compound	†XPCS,hair
R1500	T2243	T2242	punct	/,†XPCS
R1501	T2244	T2240	pobj	hair,of
R1502	T2245	T2246	det	an,appearance
R1503	T2246	T2239	dobj	appearance,revealed
R1504	T2247	T2248	advmod	almost,normal
R1505	T2248	T2246	amod	normal,appearance
R1506	T2249	T2246	punct	", ",appearance
R1507	T2250	T2246	prep	with,appearance
R1508	T2251	T2252	npadvmod	TTD,like
R1509	T2252	T2254	amod	like,features
R1510	T2253	T2252	punct	-,like
R1511	T2254	T2250	pobj	features,with
R1512	T2255	T2256	amod	such,as
R1513	T2256	T2254	prep	as,features
R1514	T2257	T2258	amod	broken,hairs
R1515	T2258	T2256	pobj	hairs,as
R1516	T2259	T2254	acl	found,features
R1517	T2260	T2261	advmod	only,at
R1518	T2261	T2259	prep	at,found
R1519	T2262	T2263	advmod	very,low
R1520	T2263	T2264	amod	low,frequency
R1521	T2264	T2261	pobj	frequency,at
R1522	T2265	T2266	punct	(,data
R1523	T2266	T2239	parataxis	data,revealed
R1524	T2267	T2266	amod	unpublished,data
R1525	T2268	T2266	punct	),data
R1526	T2269	T2239	punct	.,revealed
R1527	T2271	T2272	compound	Amino,acid
R1528	T2272	T2273	compound	acid,analysis
R1529	T2273	T2274	nsubj	analysis,confirmed
R1530	T2275	T2276	mark	that,were
R1531	T2276	T2274	ccomp	were,confirmed
R1532	T2277	T2278	compound	cysteine,levels
R1533	T2278	T2276	nsubj	levels,were
R1534	T2279	T2278	prep	in,levels
R1535	T2280	T2281	det	the,hair
R1536	T2281	T2279	pobj	hair,in
R1537	T2282	T2281	prep	of,hair
R1538	T2283	T2284	det	the,mice
R1539	T2284	T2282	pobj	mice,of
R1540	T2285	T2286	compound	XpdTTD,†XPCS
R1541	T2286	T2284	compound	†XPCS,mice
R1542	T2287	T2286	punct	/,†XPCS
R1543	T2288	T2289	advmod	significantly,higher
R1544	T2289	T2276	acomp	higher,were
R1545	T2290	T2289	prep	than,higher
R1546	T2291	T2290	prep	in,than
R1547	T2292	T2293	compound	XpdTTD,TTD
R1548	T2293	T2295	compound	TTD,animals
R1549	T2294	T2293	punct	/,TTD
R1550	T2295	T2291	pobj	animals,in
R1551	T2296	T2276	punct	", ",were
R1552	T2297	T2276	cc	but,were
R1553	T2298	T2276	conj	remained,were
R1554	T2299	T2298	prep	below,remained
R1555	T2300	T2301	det	the,level
R1556	T2301	T2299	pobj	level,below
R1557	T2302	T2301	compound	wt,level
R1558	T2303	T2304	punct	(,2C
R1559	T2304	T2274	parataxis	2C,confirmed
R1560	T2305	T2304	compound	Figure,2C
R1561	T2306	T2304	punct	),2C
R1562	T2307	T2274	punct	.,confirmed
R1563	T2309	T2310	compound	TTD,hemizygotes
R1564	T2310	T2311	nsubj	hemizygotes,display
R1565	T2312	T2313	punct	(,KO
R1566	T2313	T2310	parataxis	KO,hemizygotes
R1567	T2314	T2313	compound	XpdTTD,KO
R1568	T2315	T2313	punct	/,KO
R1569	T2316	T2313	punct	),KO
R1570	T2317	T2311	aux	do,display
R1571	T2318	T2311	neg	not,display
R1572	T2319	T2320	amod	significant,differences
R1573	T2320	T2311	dobj	differences,display
R1574	T2321	T2320	prep	in,differences
R1575	T2322	T2323	amod	cutaneous,features
R1576	T2323	T2321	pobj	features,in
R1577	T2324	T2323	cc	and,features
R1578	T2325	T2323	conj	longevity,features
R1579	T2326	T2320	amod	relative,differences
R1580	T2327	T2326	prep	to,relative
R1581	T2328	T2329	amod	homozygous,mice
R1582	T2329	T2327	pobj	mice,to
R1583	T2330	T2331	compound	XpdTTD,TTD
R1584	T2331	T2329	compound	TTD,mice
R1585	T2332	T2331	punct	/,TTD
R1586	T2333	T2334	punct	[,21
R1587	T2334	T2311	parataxis	21,display
R1588	T2335	T2334	punct	],21
R1589	T2336	T2311	punct	.,display
R1590	T2338	T2339	amod	Other,features
R1591	T2339	T2342	nsubj	features,were
R1592	T2340	T2339	amod	prominent,features
R1593	T2341	T2339	compound	TTD,features
R1594	T2343	T2339	prep	in,features
R1595	T2344	T2345	det	the,epidermis
R1596	T2345	T2343	pobj	epidermis,in
R1597	T2346	T2339	punct	", ",features
R1598	T2347	T2339	prep	including,features
R1599	T2348	T2347	pobj	acanthosis,including
R1600	T2349	T2350	punct	(,thickening
R1601	T2350	T2348	parataxis	thickening,acanthosis
R1602	T2351	T2350	prep	of,thickening
R1603	T2352	T2353	det	the,layer
R1604	T2353	T2351	pobj	layer,of
R1605	T2354	T2353	prep	of,layer
R1606	T2355	T2356	det	the,cells
R1607	T2356	T2354	pobj	cells,of
R1608	T2357	T2356	amod	nucleated,cells
R1609	T2358	T2350	punct	),thickening
R1610	T2359	T2348	punct	", ",acanthosis
R1611	T2360	T2348	conj	hyperkeratosis,acanthosis
R1612	T2361	T2362	punct	(,thickening
R1613	T2362	T2360	parataxis	thickening,hyperkeratosis
R1614	T2363	T2362	amod	prominent,thickening
R1615	T2364	T2362	prep	of,thickening
R1616	T2365	T2366	det	the,layer
R1617	T2366	T2364	pobj	layer,of
R1618	T2367	T2366	amod	cornified,layer
R1619	T2368	T2362	punct	),thickening
R1620	T2369	T2360	punct	", ",hyperkeratosis
R1621	T2370	T2360	cc	and,hyperkeratosis
R1622	T2371	T2372	amod	pronounced,layer
R1623	T2372	T2360	conj	layer,hyperkeratosis
R1624	T2373	T2372	amod	granular,layer
R1625	T2374	T2372	cc	and,layer
R1626	T2375	T2376	amod	sebacious,gland
R1627	T2376	T2377	compound	gland,hyperplasia
R1628	T2377	T2372	conj	hyperplasia,layer
R1629	T2378	T2379	punct	(,causing
R1630	T2379	T2377	parataxis	causing,hyperplasia
R1631	T2380	T2381	amod	greasy,appearance
R1632	T2381	T2379	dobj	appearance,causing
R1633	T2382	T2381	prep	of,appearance
R1634	T2383	T2384	det	the,hair
R1635	T2384	T2382	pobj	hair,of
R1636	T2385	T2379	punct	),causing
R1637	T2386	T2342	punct	", ",were
R1638	T2387	T2342	acomp	absent,were
R1639	T2388	T2342	prep	in,were
R1640	T2389	T2390	det	the,skin
R1641	T2390	T2388	pobj	skin,in
R1642	T2391	T2390	prep	of,skin
R1643	T2392	T2393	compound	XpdTTD,†XPCS
R1644	T2393	T2395	compound	†XPCS,mice
R1645	T2394	T2393	punct	/,†XPCS
R1646	T2395	T2391	pobj	mice,of
R1647	T2396	T2342	punct	", ",were
R1648	T2397	T2398	mark	as,established
R1649	T2398	T2342	advcl	established,were
R1650	T2399	T2398	prep	by,established
R1651	T2400	T2401	amod	blind,examination
R1652	T2401	T2399	pobj	examination,by
R1653	T2402	T2401	amod	microscopic,examination
R1654	T2403	T2401	prep	of,examination
R1655	T2404	T2405	compound	skin,sections
R1656	T2405	T2403	pobj	sections,of
R1657	T2406	T2407	punct	(,2B
R1658	T2407	T2342	parataxis	2B,were
R1659	T2408	T2407	compound	Figure,2B
R1660	T2409	T2407	punct	),2B
R1661	T2410	T2342	punct	.,were
R1662	T2412	T2413	advmod	Furthermore,rescued
R1663	T2414	T2413	punct	", ",rescued
R1664	T2415	T2413	nsubjpass	anaemia,rescued
R1665	T2416	T2415	cc	and,anaemia
R1666	T2417	T2418	amod	developmental,delay
R1667	T2418	T2415	conj	delay,anaemia
R1668	T2419	T2415	amod	present,anaemia
R1669	T2420	T2419	prep	in,present
R1670	T2421	T2420	pobj	patients,in
R1671	T2422	T2421	prep	with,patients
R1672	T2423	T2422	pobj	TTD,with
R1673	T2424	T2425	punct	[,24
R1674	T2425	T2421	parataxis	24,patients
R1675	T2426	T2425	punct	],24
R1676	T2427	T2420	cc	and,in
R1677	T2428	T2420	conj	in,in
R1678	T2429	T2430	compound	XpdTTD,TTD
R1679	T2430	T2432	compound	TTD,mice
R1680	T2431	T2430	punct	/,TTD
R1681	T2432	T2428	pobj	mice,in
R1682	T2433	T2434	punct	[,15
R1683	T2434	T2432	parataxis	15,mice
R1684	T2435	T2434	punct	],15
R1685	T2436	T2413	auxpass	were,rescued
R1686	T2437	T2413	advmod	both,rescued
R1687	T2438	T2413	advmod	partially,rescued
R1688	T2439	T2413	prep	in,rescued
R1689	T2440	T2441	amod	compound,mice
R1690	T2441	T2439	pobj	mice,in
R1691	T2442	T2441	amod	heterozygous,mice
R1692	T2443	T2444	compound	XpdTTD,†XPCS
R1693	T2444	T2441	compound	†XPCS,mice
R1694	T2445	T2444	punct	/,†XPCS
R1695	T2446	T2447	punct	(,2D
R1696	T2447	T2413	parataxis	2D,rescued
R1697	T2448	T2447	compound	Figure,2D
R1698	T2449	T2447	cc	and,2D
R1699	T2450	T2447	conj	2E,2D
R1700	T2451	T2447	punct	),2D
R1701	T2452	T2413	punct	.,rescued
R1706	T2577	T2578	compound	TTD,Mice
R1707	T2578	T2576	pobj	Mice,in
R1709	T2580	T2581	amod	Homozygous,Alleles
R1710	T2581	T2579	pobj	Alleles,by
R1711	T2582	T2581	amod	Lethal,Alleles
R1712	T2583	T2581	compound	Xpd,Alleles
R1713	T2585	T2586	mark	Because,share
R1714	T2586	T2605	advcl	share,addressed
R1715	T2587	T2586	nsubj	patients,share
R1716	T2588	T2587	prep	with,patients
R1717	T2589	T2588	pobj	TTD,with
R1718	T2590	T2589	punct	", ",TTD
R1719	T2591	T2589	conj	XPCS,TTD
R1720	T2592	T2591	punct	", ",XPCS
R1721	T2593	T2591	cc	and,XPCS
R1722	T2594	T2591	conj	CS,XPCS
R1723	T2595	T2589	punct	(,TTD
R1724	T2596	T2589	cc	but,TTD
R1725	T2597	T2596	neg	not,but
R1726	T2598	T2589	conj	XP,TTD
R1727	T2599	T2587	punct	),patients
R1728	T2600	T2587	cc	and,patients
R1729	T2601	T2602	det	the,models
R1730	T2602	T2587	conj	models,patients
R1731	T2603	T2602	amod	corresponding,models
R1732	T2604	T2602	compound	mouse,models
R1733	T2606	T2607	amod	similar,symptoms
R1734	T2607	T2586	dobj	symptoms,share
R1735	T2608	T2607	amod	accelerated,symptoms
R1736	T2609	T2607	compound	progeroid,symptoms
R1737	T2610	T2611	punct	[,23
R1738	T2611	T2586	parataxis	23,share
R1739	T2612	T2611	nummod	12,23
R1740	T2613	T2611	punct	",",23
R1741	T2614	T2611	nummod	13,23
R1742	T2615	T2611	punct	",",23
R1743	T2616	T2611	nummod	15,23
R1744	T2617	T2611	punct	",",23
R1745	T2618	T2611	punct	],23
R1746	T2619	T2605	punct	", ",addressed
R1747	T2620	T2605	nsubj	we,addressed
R1748	T2621	T2605	advmod	next,addressed
R1749	T2622	T2623	amod	ageing,related
R1750	T2623	T2625	amod	related,parameters
R1751	T2624	T2623	punct	-,related
R1752	T2625	T2605	dobj	parameters,addressed
R1753	T2626	T2625	prep	in,parameters
R1754	T2627	T2628	amod	compound,mice
R1755	T2628	T2626	pobj	mice,in
R1756	T2629	T2628	amod	heterozygous,mice
R1757	T2630	T2631	punct	(,Figure
R1758	T2631	T2605	parataxis	Figure,addressed
R1759	T2632	T2631	nummod	3,Figure
R1760	T2633	T2631	punct	),Figure
R1761	T2634	T2605	punct	.,addressed
R1762	T2636	T2637	mark	Whereas,show
R1763	T2637	T2642	advcl	show,were
R1764	T2638	T2639	compound	XpdTTD,TTD
R1765	T2639	T2641	compound	TTD,animals
R1766	T2640	T2639	punct	/,TTD
R1767	T2641	T2637	nsubj	animals,show
R1768	T2643	T2644	amod	reduced,density
R1769	T2644	T2637	dobj	density,show
R1770	T2645	T2646	compound	bone,mineral
R1771	T2646	T2644	compound	mineral,density
R1772	T2647	T2637	prep	as,show
R1773	T2648	T2649	det	an,indication
R1774	T2649	T2647	pobj	indication,as
R1775	T2650	T2649	prep	of,indication
R1776	T2651	T2652	det	the,onset
R1777	T2652	T2650	pobj	onset,of
R1778	T2653	T2652	amod	early,onset
R1779	T2654	T2652	prep	of,onset
R1780	T2655	T2654	pobj	osteoporosis,of
R1781	T2656	T2652	prep	before,onset
R1782	T2657	T2658	punct	~,14
R1783	T2658	T2659	nummod	14,mo
R1784	T2659	T2656	pobj	mo,before
R1785	T2660	T2659	prep	of,mo
R1786	T2661	T2660	pobj	age,of
R1787	T2662	T2663	punct	[,15
R1788	T2663	T2637	parataxis	15,show
R1789	T2664	T2663	punct	],15
R1790	T2665	T2642	punct	", ",were
R1791	T2666	T2667	compound	tail,vertebrae
R1792	T2667	T2642	nsubj	vertebrae,were
R1793	T2668	T2667	prep	from,vertebrae
R1794	T2669	T2670	nmod	compound,mice
R1795	T2670	T2668	pobj	mice,from
R1796	T2671	T2670	amod	heterozygous,mice
R1797	T2672	T2673	compound	XpdTTD,†XPCS
R1798	T2673	T2670	compound	†XPCS,mice
R1799	T2674	T2673	punct	/,†XPCS
R1800	T2675	T2642	acomp	comparable,were
R1801	T2676	T2675	prep	to,comparable
R1802	T2677	T2676	pobj	wt,to
R1803	T2678	T2679	advmod	even,at
R1804	T2679	T2642	prep	at,were
R1805	T2680	T2681	nummod	20,mo
R1806	T2681	T2679	pobj	mo,at
R1807	T2682	T2681	prep	of,mo
R1808	T2683	T2682	pobj	age,of
R1809	T2684	T2685	punct	(,3B
R1810	T2685	T2642	parataxis	3B,were
R1811	T2686	T2685	compound	Figure,3B
R1812	T2687	T2685	cc	and,3B
R1813	T2688	T2685	conj	3C,3B
R1814	T2689	T2685	punct	),3B
R1815	T2690	T2642	punct	.,were
R1816	T2692	T2693	advmod	Furthermore,3B
R1817	T2694	T2693	punct	", ",3B
R1818	T2695	T2696	mark	whereas,developed
R1819	T2696	T2693	advcl	developed,3B
R1820	T2697	T2698	compound	XpdTTD,TTD
R1821	T2698	T2700	compound	TTD,mice
R1822	T2699	T2698	punct	/,TTD
R1823	T2700	T2696	nsubj	mice,developed
R1824	T2701	T2696	dobj	kyphosis,developed
R1825	T2702	T2696	advmod	earlier,developed
R1826	T2703	T2702	prep	than,earlier
R1827	T2704	T2705	compound	wt,animals
R1828	T2705	T2703	pobj	animals,than
R1829	T2706	T2707	punct	(,onset
R1830	T2707	T2702	parataxis	onset,earlier
R1831	T2708	T2709	punct	~,3
R1832	T2709	T2710	nummod	3,mo
R1833	T2710	T2707	npadvmod	mo,onset
R1834	T2711	T2710	cc	versus,mo
R1835	T2712	T2713	quantmod	12,20
R1836	T2713	T2715	nummod	20,mo
R1837	T2714	T2713	punct	–,20
R1838	T2715	T2710	conj	mo,mo
R1839	T2716	T2707	punct	),onset
R1840	T2717	T2693	punct	", ",3B
R1841	T2718	T2719	amod	compound,mice
R1842	T2719	T2693	nsubj	mice,3B
R1843	T2720	T2719	amod	heterozygous,mice
R1844	T2721	T2722	compound	XpdTTD,†XPCS
R1845	T2722	T2719	compound	†XPCS,mice
R1846	T2723	T2722	punct	/,†XPCS
R1847	T2724	T2693	aux	did,3B
R1848	T2725	T2693	neg	not,3B
R1849	T2726	T2693	punct	(,3B
R1850	T2727	T2693	compound	Figure,3B
R1851	T2728	T2693	punct	),3B
R1852	T2729	T2693	punct	.,3B
R1853	T2731	T2732	amod	Overall,appearance
R1854	T2732	T2733	nsubj	appearance,revealed
R1855	T2734	T2732	cc	and,appearance
R1856	T2735	T2736	compound	body,weight
R1857	T2736	T2737	compound	weight,curves
R1858	T2737	T2732	conj	curves,appearance
R1859	T2738	T2739	mark	that,rescued
R1860	T2739	T2733	ccomp	rescued,revealed
R1861	T2740	T2741	npadvmod	TTD,associated
R1862	T2741	T2743	amod	associated,cachexia
R1863	T2742	T2741	punct	-,associated
R1864	T2743	T2739	nsubjpass	cachexia,rescued
R1865	T2744	T2745	npadvmod	age,related
R1866	T2745	T2743	amod	related,cachexia
R1867	T2746	T2745	punct	-,related
R1868	T2747	T2743	amod	premature,cachexia
R1869	T2748	T2743	cc	and,cachexia
R1870	T2749	T2743	conj	lack,cachexia
R1871	T2750	T2749	prep	of,lack
R1872	T2751	T2752	amod	general,fitness
R1873	T2752	T2750	pobj	fitness,of
R1874	T2753	T2739	auxpass	were,rescued
R1875	T2754	T2739	advmod	fully,rescued
R1876	T2755	T2739	prep	in,rescued
R1877	T2756	T2757	amod	compound,mice
R1878	T2757	T2755	pobj	mice,in
R1879	T2758	T2757	amod	heterozygous,mice
R1880	T2759	T2760	compound	XpdTTD,†XPCS
R1881	T2760	T2757	compound	†XPCS,mice
R1882	T2761	T2760	punct	/,†XPCS
R1883	T2762	T2763	punct	(,3A
R1884	T2763	T2733	parataxis	3A,revealed
R1885	T2764	T2763	compound	Figure,3A
R1886	T2765	T2763	cc	and,3A
R1887	T2766	T2763	conj	3D,3A
R1888	T2767	T2763	punct	),3A
R1889	T2768	T2733	punct	.,revealed
R1890	T2770	T2771	advmod	Finally,extended
R1891	T2772	T2771	punct	", ",extended
R1892	T2773	T2774	det	the,span
R1893	T2774	T2771	nsubjpass	span,extended
R1894	T2775	T2774	compound	life,span
R1895	T2776	T2774	prep	of,span
R1896	T2777	T2778	amod	compound,heterozygotes
R1897	T2778	T2776	pobj	heterozygotes,of
R1898	T2779	T2771	auxpass	was,extended
R1899	T2780	T2771	advcl	relative,extended
R1900	T2781	T2780	prep	to,relative
R1901	T2782	T2783	compound	XpdTTD,TTD
R1902	T2783	T2785	compound	TTD,mice
R1903	T2784	T2783	punct	/,TTD
R1904	T2785	T2781	pobj	mice,to
R1905	T2786	T2787	punct	(,Table
R1906	T2787	T2771	parataxis	Table,extended
R1907	T2788	T2787	nummod	2,Table
R1908	T2789	T2787	punct	),Table
R1909	T2790	T2771	punct	.,extended
R1910	T2792	T2793	aux	To,determine
R1911	T2793	T2794	advcl	determine,generated
R1912	T2795	T2796	mark	whether,was
R1913	T2796	T2793	ccomp	was,determine
R1914	T2797	T2798	det	the,allele
R1915	T2798	T2796	nsubj	allele,was
R1916	T2799	T2798	amod	homozygous,allele
R1917	T2800	T2798	amod	lethal,allele
R1918	T2801	T2798	compound	Xpd†XPCS,allele
R1919	T2802	T2796	acomp	unique,was
R1920	T2803	T2802	prep	in,unique
R1921	T2804	T2805	poss	its,ability
R1922	T2805	T2803	pobj	ability,in
R1923	T2806	T2807	aux	to,ameliorate
R1924	T2807	T2805	acl	ameliorate,ability
R1925	T2808	T2807	dobj	symptoms,ameliorate
R1926	T2809	T2808	acl	associated,symptoms
R1927	T2810	T2809	prep	with,associated
R1928	T2811	T2812	det	the,allele
R1929	T2812	T2810	pobj	allele,with
R1930	T2813	T2812	compound	XpdTTD,allele
R1931	T2814	T2794	punct	", ",generated
R1932	T2815	T2794	nsubj	we,generated
R1933	T2816	T2817	nmod	compound,mice
R1934	T2817	T2794	dobj	mice,generated
R1935	T2818	T2817	amod	heterozygous,mice
R1936	T2819	T2820	compound	XpdTTD,†XP
R1937	T2820	T2817	compound	†XP,mice
R1938	T2821	T2820	punct	/,†XP
R1939	T2822	T2794	prep	by,generated
R1940	T2823	T2822	pcomp	crossing,by
R1941	T2824	T2825	det	the,animals
R1942	T2825	T2823	dobj	animals,crossing
R1943	T2826	T2825	amod	corresponding,animals
R1944	T2827	T2825	amod	heterozygous,animals
R1945	T2828	T2794	punct	.,generated
R1946	T2830	T2831	advcl	Similar,rescued
R1947	T2832	T2830	prep	to,Similar
R1948	T2833	T2834	det	the,allele
R1949	T2834	T2832	pobj	allele,to
R1950	T2835	T2834	compound	Xpd †XPCS,allele
R1951	T2836	T2831	punct	", ",rescued
R1952	T2837	T2838	det	the,allele
R1953	T2838	T2831	nsubj	allele,rescued
R1954	T2839	T2838	amod	homozygous,allele
R1955	T2840	T2838	amod	lethal,allele
R1956	T2841	T2838	compound	Xpd †XP,allele
R1957	T2842	T2843	amod	cutaneous,symptoms
R1958	T2843	T2831	dobj	symptoms,rescued
R1959	T2844	T2843	prep	including,symptoms
R1960	T2845	T2846	compound	hair,loss
R1961	T2846	T2844	pobj	loss,including
R1962	T2847	T2848	punct	(,data
R1963	T2848	T2846	parataxis	data,loss
R1964	T2849	T2848	prep	except,data
R1965	T2850	T2849	pcomp	locally,except
R1966	T2851	T2850	prep	during,locally
R1967	T2852	T2853	det	the,round
R1968	T2853	T2851	pobj	round,during
R1969	T2854	T2853	amod	first,round
R1970	T2855	T2848	punct	;,data
R1971	T2856	T2848	amod	unpublished,data
R1972	T2857	T2848	punct	),data
R1973	T2858	T2846	punct	", ",loss
R1974	T2859	T2860	amod	reduced,content
R1975	T2860	T2846	conj	content,loss
R1976	T2861	T2860	compound	cysteine,content
R1977	T2862	T2863	punct	(,index
R1978	T2863	T2860	parataxis	index,content
R1979	T2864	T2863	compound	cysteine,index
R1980	T2865	T2863	npadvmod	9.3,index
R1981	T2866	T2865	punct	±,9.3
R1982	T2867	T2868	nummod	0.9,deviation
R1983	T2868	T2865	appos	deviation,9.3
R1984	T2869	T2868	amod	standard,deviation
R1985	T2870	T2871	punct	[,%
R1986	T2871	T2865	parataxis	%,9.3
R1987	T2872	T2871	nummod	87,%
R1988	T2873	T2871	prep	of,%
R1989	T2874	T2873	pobj	wt,of
R1990	T2875	T2871	punct	],%
R1991	T2876	T2863	punct	", ",index
R1992	T2877	T2878	nsubj	p,TTD
R1993	T2878	T2863	ccomp	TTD,index
R1994	T2879	T2878	punct	=,TTD
R1995	T2880	T2878	nummod	0.01,TTD
R1996	T2881	T2878	cc	versus,TTD
R1997	T2882	T2863	punct	),index
R1998	T2883	T2860	punct	", ",content
R1999	T2884	T2885	npadvmod	ageing,associated
R2000	T2885	T2887	amod	associated,cachexia
R2001	T2886	T2885	punct	-,associated
R2002	T2887	T2860	conj	cachexia,content
R2003	T2888	T2887	amod	premature,cachexia
R2004	T2889	T2890	punct	(,were
R2005	T2890	T2887	parataxis	were,cachexia
R2006	T2891	T2890	nsubj	males,were
R2007	T2892	T2891	cc	and,males
R2008	T2893	T2891	conj	females,males
R2009	T2894	T2895	nummod	36.1,6.4
R2010	T2895	T2897	nummod	6.4,g
R2011	T2896	T2895	punct	±,6.4
R2012	T2897	T2890	attr	g,were
R2013	T2898	T2899	punct	[,%
R2014	T2899	T2897	parataxis	%,g
R2015	T2900	T2899	nummod	93,%
R2016	T2901	T2899	prep	of,%
R2017	T2902	T2901	pobj	wt,of
R2018	T2903	T2899	punct	],%
R2019	T2904	T2897	cc	and,g
R2020	T2905	T2906	nummod	39.2,3.2
R2021	T2906	T2908	nummod	3.2,g
R2022	T2907	T2906	punct	±,3.2
R2023	T2908	T2897	conj	g,g
R2024	T2909	T2910	punct	[,%
R2025	T2910	T2908	parataxis	%,g
R2026	T2911	T2910	nummod	116,%
R2027	T2912	T2910	prep	of,%
R2028	T2913	T2912	pobj	wt,of
R2029	T2914	T2910	punct	],%
R2030	T2915	T2890	punct	", ",were
R2031	T2916	T2890	advmod	respectively,were
R2032	T2917	T2890	punct	),were
R2033	T2918	T2887	punct	", ",cachexia
R2034	T2919	T2887	cc	and,cachexia
R2035	T2920	T2921	amod	reduced,span
R2036	T2921	T2887	conj	span,cachexia
R2037	T2922	T2921	compound	life,span
R2038	T2923	T2924	punct	(,Table
R2039	T2924	T2831	parataxis	Table,rescued
R2040	T2925	T2924	nummod	2,Table
R2041	T2926	T2924	punct	),Table
R2042	T2927	T2831	punct	.,rescued
R2043	T2929	T2930	advcl	Taken,indicate
R2044	T2931	T2929	advmod	together,Taken
R2045	T2932	T2930	punct	", ",indicate
R2046	T2933	T2934	det	these,data
R2047	T2934	T2930	nsubj	data,indicate
R2048	T2935	T2936	mark	that,were
R2049	T2936	T2930	ccomp	were,indicate
R2050	T2937	T2938	nummod	two,alleles
R2051	T2938	T2936	nsubj	alleles,were
R2052	T2939	T2938	amod	independent,alleles
R2053	T2940	T2938	punct	", ",alleles
R2054	T2941	T2942	dep	which,are
R2055	T2942	T2938	relcl	are,alleles
R2056	T2943	T2942	prep	on,are
R2057	T2944	T2945	poss	their,own
R2058	T2945	T2943	pobj	own,on
R2059	T2946	T2942	acomp	unable,are
R2060	T2947	T2948	aux	to,support
R2061	T2948	T2946	xcomp	support,unable
R2062	T2949	T2948	dobj	viability,support
R2063	T2950	T2951	punct	(,Table
R2064	T2951	T2948	parataxis	Table,support
R2065	T2952	T2951	nummod	1,Table
R2066	T2953	T2951	punct	),Table
R2067	T2954	T2936	punct	", ",were
R2068	T2955	T2936	advmod	nonetheless,were
R2069	T2956	T2936	acomp	able,were
R2070	T2957	T2958	aux	to,ameliorate
R2071	T2958	T2956	xcomp	ameliorate,able
R2072	T2959	T2960	npadvmod	TTD,associated
R2073	T2960	T2962	amod	associated,phenotypes
R2074	T2961	T2960	punct	-,associated
R2075	T2962	T2958	dobj	phenotypes,ameliorate
R2076	T2963	T2964	advmod	in,vivo
R2077	T2964	T2958	advmod	vivo,ameliorate
R2078	T2965	T2966	punct	(,Table
R2079	T2966	T2958	parataxis	Table,ameliorate
R2080	T2967	T2966	nummod	2,Table
R2081	T2968	T2966	punct	),Table
R2082	T2969	T2930	punct	.,indicate
R2083	T3312	T3313	amod	Molecular,Mechanisms
R2084	T3314	T3313	prep	of,Mechanisms
R2085	T3315	T3316	amod	Biallelic,Effects
R2086	T3316	T3314	pobj	Effects,of
R2087	T3318	T3319	nsubj	We,turned
R2088	T3320	T3319	advmod	next,turned
R2089	T3321	T3319	prep	to,turned
R2090	T3322	T3323	npadvmod	UV,based
R2091	T3323	T3325	amod	based,assays
R2092	T3324	T3323	punct	-,based
R2093	T3325	T3321	pobj	assays,to
R2094	T3326	T3325	amod	cellular,assays
R2095	T3327	T3325	prep	including,assays
R2096	T3328	T3329	amod	unscheduled,synthesis
R2097	T3329	T3327	pobj	synthesis,including
R2098	T3330	T3329	compound	DNA,synthesis
R2099	T3331	T3329	prep	after,synthesis
R2100	T3332	T3333	compound	UV,irradiation
R2101	T3333	T3331	pobj	irradiation,after
R2102	T3334	T3335	punct	(,UDS
R2103	T3335	T3329	parataxis	UDS,synthesis
R2104	T3336	T3335	compound	UV,UDS
R2105	T3337	T3335	punct	-,UDS
R2106	T3338	T3335	punct	),UDS
R2107	T3339	T3329	punct	", ",synthesis
R2108	T3340	T3329	conj	recovery,synthesis
R2109	T3341	T3340	prep	of,recovery
R2110	T3342	T3343	compound	RNA,synthesis
R2111	T3343	T3341	pobj	synthesis,of
R2112	T3344	T3340	prep	after,recovery
R2113	T3345	T3346	compound	UV,irradiation
R2114	T3346	T3344	pobj	irradiation,after
R2115	T3347	T3348	punct	(,RRS
R2116	T3348	T3340	parataxis	RRS,recovery
R2117	T3349	T3348	compound	UV,RRS
R2118	T3350	T3348	punct	-,RRS
R2119	T3351	T3348	punct	),RRS
R2120	T3352	T3340	punct	", ",recovery
R2121	T3353	T3340	cc	and,recovery
R2122	T3354	T3355	compound	UV,survival
R2123	T3355	T3340	conj	survival,recovery
R2124	T3356	T3329	punct	", ",synthesis
R2125	T3357	T3358	dep	which,report
R2126	T3358	T3329	relcl	report,synthesis
R2127	T3359	T3358	prep	on,report
R2128	T3360	T3361	det	the,subpathways
R2129	T3361	T3359	pobj	subpathways,on
R2130	T3362	T3361	compound	NER,subpathways
R2131	T3363	T3364	punct	(,NER
R2132	T3364	T3361	parataxis	NER,subpathways
R2133	T3365	T3364	amod	global,NER
R2134	T3366	T3364	compound	genome,NER
R2135	T3367	T3364	cc	and,NER
R2136	T3368	T3369	npadvmod	transcription,coupled
R2137	T3369	T3371	amod	coupled,NER
R2138	T3370	T3369	punct	-,coupled
R2139	T3371	T3364	conj	NER,NER
R2140	T3372	T3364	punct	),NER
R2141	T3373	T3361	cc	and,subpathways
R2142	T3374	T3375	amod	total,NER
R2143	T3375	T3361	conj	NER,subpathways
R2144	T3376	T3358	punct	", ",report
R2145	T3377	T3358	advmod	respectively,report
R2146	T3378	T3319	punct	.,turned
R2147	T3380	T3381	prep	In,improved
R2148	T3382	T3380	pobj	none,In
R2149	T3383	T3382	prep	of,none
R2150	T3384	T3385	det	these,assays
R2151	T3385	T3383	pobj	assays,of
R2152	T3386	T3381	auxpass	was,improved
R2153	T3387	T3388	det	the,response
R2154	T3388	T3381	nsubjpass	response,improved
R2155	T3389	T3388	prep	to,response
R2156	T3390	T3389	pobj	UV,to
R2157	T3391	T3381	prep	in,improved
R2158	T3392	T3393	compound	compound,heterozygotes
R2159	T3393	T3391	pobj	heterozygotes,in
R2160	T3394	T3381	advcl	relative,improved
R2161	T3395	T3394	prep	to,relative
R2162	T3396	T3397	compound	TTD,homozygotes
R2163	T3397	T3395	pobj	homozygotes,to
R2164	T3398	T3399	punct	(,4A
R2165	T3399	T3381	parataxis	4A,improved
R2166	T3400	T3399	compound	Figure,4A
R2167	T3401	T3402	punct	–,4C
R2168	T3402	T3399	prep	4C,4A
R2169	T3403	T3399	punct	),4A
R2170	T3404	T3381	punct	.,improved
R2171	T3406	T3407	advmod	However,observed
R2172	T3408	T3407	punct	", ",observed
R2173	T3409	T3407	prep	unlike,observed
R2174	T3410	T3411	det	the,phenotypes
R2175	T3411	T3409	pobj	phenotypes,unlike
R2176	T3412	T3413	advmod	in,vivo
R2177	T3413	T3411	amod	vivo,phenotypes
R2178	T3414	T3411	compound	TTD,phenotypes
R2179	T3415	T3411	acl	described,phenotypes
R2180	T3416	T3415	advmod	above,described
R2181	T3417	T3411	punct	", ",phenotypes
R2182	T3418	T3419	prep	in,were
R2183	T3419	T3411	relcl	were,phenotypes
R2184	T3420	T3418	pobj	which,in
R2185	T3421	T3422	nmod	XpdTTD,TTD
R2186	T3422	T3424	nmod	TTD,animals
R2187	T3423	T3422	punct	/,TTD
R2188	T3424	T3419	nsubj	animals,were
R2189	T3425	T3422	cc	and,TTD
R2190	T3426	T3427	compound	XpdTTD,KO
R2191	T3427	T3422	conj	KO,TTD
R2192	T3428	T3427	punct	/,KO
R2193	T3429	T3419	acomp	indistinguishable,were
R2194	T3430	T3407	punct	", ",observed
R2195	T3431	T3432	compound	XpdTTD,effects
R2196	T3432	T3407	nsubjpass	effects,observed
R2197	T3433	T3432	compound	dosage,effects
R2198	T3434	T3407	auxpass	were,observed
R2199	T3435	T3407	prep	in,observed
R2200	T3436	T3437	compound	UV,survival
R2201	T3437	T3435	pobj	survival,in
R2202	T3438	T3437	punct	", ",survival
R2203	T3439	T3440	compound	UV,UDS
R2204	T3440	T3437	conj	UDS,survival
R2205	T3441	T3440	punct	-,UDS
R2206	T3442	T3440	punct	", ",UDS
R2207	T3443	T3440	cc	and,UDS
R2208	T3444	T3445	compound	UV,RRS
R2209	T3445	T3440	conj	RRS,UDS
R2210	T3446	T3445	punct	-,RRS
R2211	T3447	T3407	punct	", ",observed
R2212	T3448	T3407	advcl	indicating,observed
R2213	T3449	T3450	mark	that,correlate
R2214	T3450	T3448	ccomp	correlate,indicating
R2215	T3451	T3452	amod	cellular,parameters
R2216	T3452	T3450	nsubj	parameters,correlate
R2217	T3453	T3454	mark	as,measured
R2218	T3454	T3452	advcl	measured,parameters
R2219	T3455	T3454	prep	in,measured
R2220	T3456	T3455	pobj	fibroblasts,in
R2221	T3457	T3454	advmod	here,measured
R2222	T3458	T3450	aux	do,correlate
R2223	T3459	T3450	neg	not,correlate
R2224	T3460	T3450	advmod	always,correlate
R2225	T3461	T3450	prep	with,correlate
R2226	T3462	T3463	det	the,phenotype
R2227	T3463	T3461	pobj	phenotype,with
R2228	T3464	T3450	prep	at,correlate
R2229	T3465	T3466	det	the,level
R2230	T3466	T3464	pobj	level,at
R2231	T3467	T3466	prep	of,level
R2232	T3468	T3469	det	the,organism
R2233	T3469	T3467	pobj	organism,of
R2234	T3470	T3469	amod	intact,organism
R2235	T3471	T3407	punct	.,observed
R2236	T3473	T3474	nmod	XpdTTD,KO
R2237	T3474	T3476	nmod	KO,cells
R2238	T3475	T3474	punct	/,KO
R2239	T3476	T3478	nsubjpass	cells,used
R2240	T3477	T3476	amod	hemizygous,cells
R2241	T3479	T3478	auxpass	were,used
R2242	T3480	T3478	advmod	thus,used
R2243	T3481	T3478	prep	as,used
R2244	T3482	T3483	det	the,baseline
R2245	T3483	T3481	pobj	baseline,as
R2246	T3484	T3485	prep	on,compare
R2247	T3485	T3483	relcl	compare,baseline
R2248	T3486	T3484	pobj	which,on
R2249	T3487	T3485	aux	to,compare
R2250	T3488	T3489	det	the,activity
R2251	T3489	T3485	dobj	activity,compare
R2252	T3490	T3489	prep	of,activity
R2253	T3491	T3492	nmod	compound,cells
R2254	T3492	T3490	pobj	cells,of
R2255	T3493	T3492	amod	heterozygous,cells
R2256	T3494	T3478	punct	.,used
R2257	T3496	T3497	advcl	Relative,improved
R2258	T3498	T3496	prep	to,Relative
R2259	T3499	T3500	compound	XpdTTD,KO
R2260	T3500	T3502	compound	KO,cells
R2261	T3501	T3500	punct	/,KO
R2262	T3502	T3498	pobj	cells,to
R2263	T3503	T3502	compound	hemizygote,cells
R2264	T3504	T3497	punct	", ",improved
R2265	T3505	T3506	compound	UV,survival
R2266	T3506	T3497	nsubjpass	survival,improved
R2267	T3507	T3497	auxpass	was,improved
R2268	T3508	T3497	prep	by,improved
R2269	T3509	T3510	det	the,allele
R2270	T3510	T3508	pobj	allele,by
R2271	T3511	T3510	amod	homozygous,allele
R2272	T3512	T3510	amod	lethal,allele
R2273	T3513	T3510	compound	Xpd†XPCS,allele
R2274	T3514	T3510	prep	in,allele
R2275	T3515	T3516	nmod	XpdTTD,†XPCS
R2276	T3516	T3518	nmod	†XPCS,cells
R2277	T3517	T3516	punct	/,†XPCS
R2278	T3518	T3514	pobj	cells,in
R2279	T3519	T3518	nmod	compound,cells
R2280	T3520	T3518	amod	heterozygous,cells
R2281	T3521	T3508	cc	and,by
R2282	T3522	T3523	prep	to,by
R2283	T3523	T3508	conj	by,by
R2284	T3524	T3525	det	a,degree
R2285	T3525	T3522	pobj	degree,to
R2286	T3526	T3525	amod	lesser,degree
R2287	T3527	T3528	det	the,allele
R2288	T3528	T3523	pobj	allele,by
R2289	T3529	T3528	compound	Xpd†XP,allele
R2290	T3530	T3531	punct	(,4A
R2291	T3531	T3497	parataxis	4A,improved
R2292	T3532	T3531	compound	Figure,4A
R2293	T3533	T3531	punct	),4A
R2294	T3534	T3497	punct	.,improved
R2295	T3536	T3537	prep	Because,were
R2296	T3538	T3536	pcomp	of,Because
R2297	T3539	T3540	amod	embryonic,lethality
R2298	T3540	T3536	pobj	lethality,Because
R2299	T3541	T3539	cc	and,embryonic
R2300	T3542	T3539	conj	cellular,embryonic
R2301	T3543	T3537	punct	", ",were
R2302	T3544	T3537	nsubj	we,were
R2303	T3545	T3537	acomp	unable,were
R2304	T3546	T3547	aux	to,test
R2305	T3547	T3545	xcomp	test,unable
R2306	T3548	T3549	compound	UV,survival
R2307	T3549	T3547	dobj	survival,test
R2308	T3550	T3549	acl	associated,survival
R2309	T3551	T3550	advmod	exclusively,associated
R2310	T3552	T3550	prep	with,associated
R2311	T3553	T3554	det	the,alleles
R2312	T3554	T3552	pobj	alleles,with
R2313	T3555	T3554	nmod	Xpd†XPCS,alleles
R2314	T3556	T3555	cc	or,Xpd†XPCS
R2315	T3557	T3555	conj	Xpd†XP,Xpd†XPCS
R2316	T3558	T3537	punct	.,were
R2317	T3560	T3561	advmod	However,known
R2318	T3562	T3561	punct	", ",known
R2319	T3563	T3564	amod	homozygous,XPDXP
R2320	T3564	T3565	nmod	XPDXP,cells
R2321	T3565	T3561	nsubjpass	cells,known
R2322	T3566	T3567	punct	(,XPDR683W
R2323	T3567	T3564	parataxis	XPDR683W,XPDXP
R2324	T3568	T3567	punct	),XPDR683W
R2325	T3569	T3564	cc	and,XPDXP
R2326	T3570	T3571	amod	hemizygous,XPDXPCS
R2327	T3571	T3564	conj	XPDXPCS,XPDXP
R2328	T3572	T3573	punct	(,XPDG602D
R2329	T3573	T3571	parataxis	XPDG602D,XPDXPCS
R2330	T3574	T3573	punct	),XPDG602D
R2331	T3575	T3565	amod	human,cells
R2332	T3576	T3561	auxpass	are,known
R2333	T3577	T3578	aux	to,be
R2334	T3578	T3561	xcomp	be,known
R2335	T3579	T3580	advmod	highly,sensitive
R2336	T3580	T3578	acomp	sensitive,be
R2337	T3581	T3580	prep	to,sensitive
R2338	T3582	T3581	pobj	UV,to
R2339	T3583	T3584	punct	[,25
R2340	T3584	T3578	parataxis	25,be
R2341	T3585	T3584	nummod	19,25
R2342	T3586	T3584	punct	",",25
R2343	T3587	T3584	punct	],25
R2344	T3588	T3561	punct	", ",known
R2345	T3589	T3590	mark	as,are
R2346	T3590	T3561	advcl	are,known
R2347	T3591	T3590	nsubj	cells,are
R2348	T3592	T3591	prep	from,cells
R2349	T3593	T3594	det	a,model
R2350	T3594	T3592	pobj	model,from
R2351	T3595	T3594	amod	homozygous,model
R2352	T3596	T3594	amod	viable,model
R2353	T3597	T3598	nmod	XpdXPCS,XPCS
R2354	T3598	T3594	nmod	XPCS,model
R2355	T3599	T3598	punct	/,XPCS
R2356	T3600	T3601	punct	(,G602D
R2357	T3601	T3598	parataxis	G602D,XPCS
R2358	T3602	T3601	compound	XPDG602D,G602D
R2359	T3603	T3601	punct	/,G602D
R2360	T3604	T3601	punct	),G602D
R2361	T3605	T3594	compound	mouse,model
R2362	T3606	T3607	punct	(,4A
R2363	T3607	T3590	parataxis	4A,are
R2364	T3608	T3607	compound	Figure,4A
R2365	T3609	T3607	punct	", ",4A
R2366	T3610	T3611	amod	dotted,line
R2367	T3611	T3607	appos	line,4A
R2368	T3612	T3607	punct	),4A
R2369	T3613	T3614	punct	[,23
R2370	T3614	T3561	parataxis	23,known
R2371	T3615	T3614	punct	],23
R2372	T3616	T3561	punct	.,known
R2373	T3618	T3619	advmod	Thus,represents
R2374	T3620	T3619	punct	", ",represents
R2375	T3621	T3622	det	the,survival
R2376	T3622	T3619	nsubj	survival,represents
R2377	T3623	T3622	prep	of,survival
R2378	T3624	T3625	nmod	XpdTTD,†XPCS
R2379	T3625	T3627	nmod	†XPCS,cells
R2380	T3626	T3625	punct	/,†XPCS
R2381	T3627	T3623	pobj	cells,of
R2382	T3628	T3625	punct	(,†XPCS
R2383	T3629	T3625	cc	and,†XPCS
R2384	T3630	T3631	compound	XpdTTD,†XP
R2385	T3631	T3625	conj	†XP,†XPCS
R2386	T3632	T3631	punct	/,†XP
R2387	T3633	T3627	punct	),cells
R2388	T3634	T3619	advmod	likely,represents
R2389	T3635	T3636	det	a,level
R2390	T3636	T3619	dobj	level,represents
R2391	T3637	T3636	prep	of,level
R2392	T3638	T3639	compound	UV,resistance
R2393	T3639	T3637	pobj	resistance,of
R2394	T3640	T3641	dep	that,impart
R2395	T3641	T3636	relcl	impart,level
R2396	T3642	T3643	preconj	neither,allele
R2397	T3643	T3641	nsubj	allele,impart
R2398	T3644	T3643	amod	mutant,allele
R2399	T3645	T3641	aux	can,impart
R2400	T3646	T3641	prep	on,impart
R2401	T3647	T3648	poss	its,own
R2402	T3648	T3646	pobj	own,on
R2403	T3649	T3650	punct	(,Table
R2404	T3650	T3619	parataxis	Table,represents
R2405	T3651	T3650	nummod	2,Table
R2406	T3652	T3650	punct	),Table
R2407	T3653	T3619	punct	.,represents
R2408	T3655	T3656	amod	Significant,effects
R2409	T3656	T3657	nsubjpass	effects,observed
R2410	T3658	T3656	prep	of,effects
R2411	T3659	T3660	compound	compound,heterozygosity
R2412	T3660	T3658	pobj	heterozygosity,of
R2413	T3661	T3656	prep	on,effects
R2414	T3662	T3663	compound	NER,subpathways
R2415	T3663	T3661	pobj	subpathways,on
R2416	T3664	T3656	amod	relative,effects
R2417	T3665	T3664	prep	to,relative
R2418	T3666	T3667	compound	XpdTTD,KO
R2419	T3667	T3669	compound	KO,cells
R2420	T3668	T3667	punct	/,KO
R2421	T3669	T3665	pobj	cells,to
R2422	T3670	T3657	auxpass	were,observed
R2423	T3671	T3657	prep	in,observed
R2424	T3672	T3673	compound	XpdTTD,†XP
R2425	T3673	T3675	compound	†XP,cells
R2426	T3674	T3673	punct	/,†XP
R2427	T3675	T3671	pobj	cells,in
R2428	T3676	T3671	cc	but,in
R2429	T3677	T3678	advmod	only,for
R2430	T3678	T3671	conj	for,in
R2431	T3679	T3680	compound	UV,UDS
R2432	T3680	T3682	compound	UDS,activity
R2433	T3681	T3680	punct	-,UDS
R2434	T3682	T3678	pobj	activity,for
R2435	T3683	T3657	punct	.,observed
R2436	T3685	T3686	advmod	Finally,exhibited
R2437	T3687	T3686	punct	", ",exhibited
R2438	T3688	T3686	nsubj	none,exhibited
R2439	T3689	T3688	prep	of,none
R2440	T3690	T3691	det	the,combinations
R2441	T3691	T3689	pobj	combinations,of
R2442	T3692	T3691	amod	mutant,combinations
R2443	T3693	T3691	compound	TFIIH,combinations
R2444	T3694	T3691	punct	(,combinations
R2445	T3695	T3691	acl	carrying,combinations
R2446	T3696	T3695	dobj	alterations,carrying
R2447	T3697	T3696	acl	associated,alterations
R2448	T3698	T3697	prep	with,associated
R2449	T3699	T3698	pobj	TTD,with
R2450	T3700	T3701	punct	[,XPDR722W
R2451	T3701	T3699	parataxis	XPDR722W,TTD
R2452	T3702	T3701	punct	],XPDR722W
R2453	T3703	T3699	punct	", ",TTD
R2454	T3704	T3699	conj	XPCS,TTD
R2455	T3705	T3706	punct	[,XPDG602D
R2456	T3706	T3704	parataxis	XPDG602D,XPCS
R2457	T3707	T3706	punct	],XPDG602D
R2458	T3708	T3704	punct	", ",XPCS
R2459	T3709	T3704	cc	or,XPCS
R2460	T3710	T3704	conj	XP,XPCS
R2461	T3711	T3712	punct	[,XPDR683W
R2462	T3712	T3710	parataxis	XPDR683W,XP
R2463	T3713	T3712	punct	],XPDR683W
R2464	T3714	T3686	punct	),exhibited
R2465	T3715	T3686	dobj	synergism,exhibited
R2466	T3716	T3686	prep	in,exhibited
R2467	T3717	T3718	det	an,reaction
R2468	T3718	T3716	pobj	reaction,in
R2469	T3719	T3720	advmod	in,vitro
R2470	T3720	T3718	amod	vitro,reaction
R2471	T3721	T3718	compound	NER,reaction
R2472	T3722	T3718	acl	reconstituted,reaction
R2473	T3723	T3722	prep	with,reconstituted
R2474	T3724	T3725	amod	different,complexes
R2475	T3725	T3723	pobj	complexes,with
R2476	T3726	T3725	amod	mutant,complexes
R2477	T3727	T3725	compound	TFIIH,complexes
R2478	T3728	T3729	punct	(,4D
R2479	T3729	T3686	parataxis	4D,exhibited
R2480	T3730	T3729	compound	Figure,4D
R2481	T3731	T3729	punct	),4D
R2482	T3732	T3686	punct	.,exhibited
R2483	T3734	T3735	advcl	Taken,are
R2484	T3736	T3734	advmod	together,Taken
R2485	T3737	T3735	punct	", ",are
R2486	T3738	T3739	det	these,data
R2487	T3739	T3735	nsubj	data,are
R2488	T3740	T3735	acomp	consistent,are
R2489	T3741	T3740	prep	with,consistent
R2490	T3742	T3743	amod	interallelic,complementation
R2491	T3743	T3741	pobj	complementation,with
R2492	T3744	T3743	prep	of,complementation
R2493	T3745	T3746	compound	UV,sensitivity
R2494	T3746	T3744	pobj	sensitivity,of
R2495	T3747	T3743	prep	in,complementation
R2496	T3748	T3747	pobj	cells,in
R2497	T3749	T3735	cc	but,are
R2498	T3750	T3735	conj	underscore,are
R2499	T3751	T3752	det	the,lack
R2500	T3752	T3750	dobj	lack,underscore
R2501	T3753	T3752	prep	of,lack
R2502	T3754	T3755	det	any,correlation
R2503	T3755	T3753	pobj	correlation,of
R2504	T3756	T3755	prep	between,correlation
R2505	T3757	T3758	npadvmod	UV,related
R2506	T3758	T3760	amod	related,characteristics
R2507	T3759	T3758	punct	-,related
R2508	T3760	T3756	pobj	characteristics,between
R2509	T3761	T3760	compound	repair,characteristics
R2510	T3762	T3760	cc	and,characteristics
R2511	T3763	T3764	compound	TTD,phenotypes
R2512	T3764	T3760	conj	phenotypes,characteristics
R2513	T3765	T3764	compound	progeroid,phenotypes
R2514	T3766	T3755	prep	in,correlation
R2515	T3767	T3768	compound	animal,models
R2516	T3768	T3766	pobj	models,in
R2517	T3769	T3735	punct	.,are
R2518	T3778	T3779	nsubj	sk,e
R2519	T3779	T3780	ccomp	e,t
R2520	T3781	T3779	dobj	d,e
R2521	T3782	T3781	prep	wh,d
R2522	T3783	T3780	nsubj	e,t
R2523	T3789	T3790	det	h,e
R2524	T3798	T3797	cc	and,†XPCS
R2525	T3799	T3800	compound	Xpd,†XP
R2526	T3800	T3797	conj	†XP,†XPCS
R2527	T3801	T3802	amod	a,l
R2528	T3804	T3805	nsubj	l,e
R2529	T3806	T3805	dobj	les,e
R2530	T3812	T3811	pobj	e,it
R2531	T3817	T3818	amod	re,a
R2532	T3821	T3820	cc	d,e
R2533	T3822	T3820	conj	m,e
R2534	T3826	T3825	compound	RNA,levels
R2535	T3827	T3824	punct	", ",ameliorated
R2536	T3828	T3829	compound	TTD,symptoms
R2537	T3829	T3824	dobj	symptoms,ameliorated
R2538	T3830	T3824	prep	by,ameliorated
R2539	T3831	T3832	amod	increasing,levels
R2540	T3832	T3830	pobj	levels,by
R2541	T3833	T3832	amod	overall,levels
R2542	T3834	T3832	compound	TFIIH,levels
R2543	T3835	T3824	prep	in,ameliorated
R2544	T3836	T3837	nmod	compound,cells
R2545	T3837	T3835	pobj	cells,in
R2546	T3838	T3837	amod	heterozygous,cells
R2547	T3839	T3840	nmod	XpdTTD, †XPCS
R2548	T3840	T3837	nmod	†XPCS,cells
R2549	T3841	T3840	punct	/, †XPCS
R2550	T3842	T3840	cc	and, †XPCS
R2551	T3843	T3844	compound	XpdTTD, †XP
R2552	T3844	T3840	conj	†XP, †XPCS
R2553	T3845	T3844	punct	/, †XP
R2554	T3848	T3849	advmod	Previously,shown
R2555	T3850	T3849	punct	", ",shown
R2556	T3851	T3849	advcl	using,shown
R2557	T3852	T3853	amod	comparative,immunohistochemistry
R2558	T3853	T3851	dobj	immunohistochemistry,using
R2559	T3854	T3849	punct	", ",shown
R2560	T3855	T3849	nsubj	we,shown
R2561	T3856	T3855	cc	and,we
R2562	T3857	T3855	conj	others,we
R2563	T3858	T3849	aux	have,shown
R2564	T3859	T3860	det	an,reduction
R2565	T3860	T3849	dobj	reduction,shown
R2566	T3861	T3862	quantmod	up,70
R2567	T3862	T3864	nummod	70,%
R2568	T3863	T3862	quantmod	to,70
R2569	T3864	T3860	compound	%,reduction
R2570	T3865	T3860	prep	of,reduction
R2571	T3866	T3867	compound	TFIIH,levels
R2572	T3867	T3865	pobj	levels,of
R2573	T3868	T3867	prep	in,levels
R2574	T3869	T3870	amod	primary,fibroblasts
R2575	T3870	T3868	pobj	fibroblasts,in
R2576	T3871	T3870	prep	from,fibroblasts
R2577	T3872	T3871	pobj	patients,from
R2578	T3873	T3872	prep	with,patients
R2579	T3874	T3873	pobj	TTD,with
R2580	T3875	T3860	prep	compared,reduction
R2581	T3876	T3875	prep	with,compared
R2582	T3877	T3878	compound	wt,controls
R2583	T3878	T3876	pobj	controls,with
R2584	T3879	T3860	prep	due,reduction
R2585	T3880	T3879	pcomp	to,due
R2586	T3881	T3882	amod	reduced,stability
R2587	T3882	T3879	pobj	stability,due
R2588	T3883	T3884	punct	[,17
R2589	T3884	T3849	parataxis	17,shown
R2590	T3885	T3884	nummod	16,17
R2591	T3886	T3884	punct	",",17
R2592	T3887	T3884	punct	],17
R2593	T3888	T3849	punct	.,shown
R2594	T3890	T3891	prep	Despite,reduced
R2595	T3892	T3890	pobj	overexpression,Despite
R2596	T3893	T3892	prep	of,overexpression
R2597	T3894	T3893	pobj	mRNA,of
R2598	T3895	T3894	prep	from,mRNA
R2599	T3896	T3897	det	the,allele
R2600	T3897	T3895	pobj	allele,from
R2601	T3898	T3897	compound	XpdTTD,allele
R2602	T3899	T3892	amod	relative,overexpression
R2603	T3900	T3899	prep	to,relative
R2604	T3901	T3902	det	the,allele
R2605	T3902	T3900	pobj	allele,to
R2606	T3903	T3902	compound	wt,allele
R2607	T3904	T3905	punct	(,1E
R2608	T3905	T3892	parataxis	1E,overexpression
R2609	T3906	T3905	compound	Figure,1E
R2610	T3907	T3905	punct	),1E
R2611	T3908	T3891	punct	", ",reduced
R2612	T3909	T3910	compound	TFIIH,levels
R2613	T3910	T3891	nsubjpass	levels,reduced
R2614	T3911	T3910	compound	protein,levels
R2615	T3912	T3891	auxpass	were,reduced
R2616	T3913	T3891	prep	by,reduced
R2617	T3914	T3915	nummod	50,%
R2618	T3915	T3913	pobj	%,by
R2619	T3916	T3891	prep	in,reduced
R2620	T3917	T3918	amod	primary,fibroblasts
R2621	T3918	T3916	pobj	fibroblasts,in
R2622	T3919	T3918	compound	mouse,fibroblasts
R2623	T3920	T3921	compound	XpdTTD,TTD
R2624	T3921	T3918	compound	TTD,fibroblasts
R2625	T3922	T3921	punct	/,TTD
R2626	T3923	T3924	punct	(,4E
R2627	T3924	T3891	parataxis	4E,reduced
R2628	T3925	T3924	compound	Figure,4E
R2629	T3926	T3924	cc	and,4E
R2630	T3927	T3924	conj	4F,4E
R2631	T3928	T3924	punct	),4E
R2632	T3929	T3891	punct	", ",reduced
R2633	T3930	T3931	advmod	thereby,mimicking
R2634	T3931	T3891	advcl	mimicking,reduced
R2635	T3932	T3933	det	the,situation
R2636	T3933	T3931	dobj	situation,mimicking
R2637	T3934	T3931	prep	in,mimicking
R2638	T3935	T3936	amod	human,patients
R2639	T3936	T3934	pobj	patients,in
R2640	T3937	T3936	prep	with,patients
R2641	T3938	T3937	pobj	TTD,with
R2642	T3939	T3891	punct	.,reduced
R2643	T3941	T3942	prep	In,observed
R2644	T3943	T3941	pobj	accordance,In
R2645	T3944	T3943	prep	with,accordance
R2646	T3945	T3946	det	the,dosage
R2647	T3946	T3944	pobj	dosage,with
R2648	T3947	T3946	compound	gene,dosage
R2649	T3948	T3942	punct	", ",observed
R2650	T3949	T3950	det	a,reduction
R2651	T3950	T3942	nsubjpass	reduction,observed
R2652	T3951	T3950	amod	further,reduction
R2653	T3952	T3950	prep	of,reduction
R2654	T3953	T3954	quantmod	up,70
R2655	T3954	T3956	nummod	70,%
R2656	T3955	T3954	quantmod	to,70
R2657	T3956	T3952	pobj	%,of
R2658	T3957	T3956	prep	of,%
R2659	T3958	T3959	det	the,level
R2660	T3959	T3957	pobj	level,of
R2661	T3960	T3959	compound	wt,level
R2662	T3961	T3942	auxpass	was,observed
R2663	T3962	T3942	prep	in,observed
R2664	T3963	T3964	amod	hemizygous,cells
R2665	T3964	T3962	pobj	cells,in
R2666	T3965	T3966	compound	XpdTTD,KO
R2667	T3966	T3964	compound	KO,cells
R2668	T3967	T3966	punct	/,KO
R2669	T3968	T3942	punct	.,observed
R2670	T3970	T3971	advcl	Consistent,was
R2671	T3972	T3970	prep	with,Consistent
R2672	T3973	T3974	amod	low,levels
R2673	T3974	T3972	pobj	levels,with
R2674	T3975	T3976	compound	mRNA,expression
R2675	T3976	T3974	compound	expression,levels
R2676	T3977	T3971	punct	", ",was
R2677	T3978	T3979	preconj	neither,allele
R2678	T3979	T3971	nsubj	allele,was
R2679	T3980	T3981	det	the,Xpd†XPCS
R2680	T3981	T3979	nmod	Xpd†XPCS,allele
R2681	T3982	T3981	cc	nor,Xpd†XPCS
R2682	T3983	T3984	det	the,Xpd†XP
R2683	T3984	T3981	conj	Xpd†XP,Xpd†XPCS
R2684	T3985	T3971	acomp	able,was
R2685	T3986	T3987	aux	to,restore
R2686	T3987	T3985	xcomp	restore,able
R2687	T3988	T3989	compound	TFIIH,abundance
R2688	T3989	T3987	dobj	abundance,restore
R2689	T3990	T3987	prep	to,restore
R2690	T3991	T3992	compound	wt,levels
R2691	T3992	T3990	pobj	levels,to
R2692	T3993	T3987	prep	in,restore
R2693	T3994	T3995	compound	XpdTTD,cells
R2694	T3995	T3993	pobj	cells,in
R2695	T3996	T3995	compound	compound,cells
R2696	T3997	T3995	compound	heterozygote,cells
R2697	T3998	T3999	punct	(,4E
R2698	T3999	T3971	parataxis	4E,was
R2699	T4000	T3999	compound	Figure,4E
R2700	T4001	T3999	cc	and,4E
R2701	T4002	T3999	conj	4F,4E
R2702	T4003	T3999	punct	),4E
R2703	T4004	T3971	punct	.,was
R2704	T4006	T4007	advmod	Thus,were
R2705	T4008	T4007	punct	", ",were
R2706	T4009	T4010	det	the,survival
R2707	T4010	T4007	nsubj	survival,were
R2708	T4011	T4010	amod	improved,survival
R2709	T4012	T4010	compound	UV,survival
R2710	T4013	T4010	acl	observed,survival
R2711	T4014	T4013	prep	in,observed
R2712	T4015	T4016	compound	compound,cells
R2713	T4016	T4014	pobj	cells,in
R2714	T4017	T4016	compound	heterozygote,cells
R2715	T4018	T4019	punct	(,4A
R2716	T4019	T4013	parataxis	4A,observed
R2717	T4020	T4019	compound	Figure,4A
R2718	T4021	T4019	punct	),4A
R2719	T4022	T4013	cc	and,observed
R2720	T4023	T4024	advmod	likely,rescue
R2721	T4024	T4013	conj	rescue,observed
R2722	T4025	T4024	det	the,rescue
R2723	T4026	T4024	prep	of,rescue
R2724	T4027	T4028	compound	TTD,symptoms
R2725	T4028	T4026	pobj	symptoms,of
R2726	T4029	T4028	compound	progeroid,symptoms
R2727	T4030	T4031	punct	(,Figure
R2728	T4031	T4024	parataxis	Figure,rescue
R2729	T4032	T4031	nummod	3,Figure
R2730	T4033	T4031	punct	),Figure
R2731	T4034	T4007	neg	not,were
R2732	T4035	T4007	prep	due,were
R2733	T4036	T4035	pcomp	to,due
R2734	T4037	T4035	pobj	normalisation,due
R2735	T4038	T4037	prep	of,normalisation
R2736	T4039	T4040	compound	TFIIH,levels
R2737	T4040	T4038	pobj	levels,of
R2738	T4041	T4007	punct	", ",were
R2739	T4042	T4007	advcl	suggesting,were
R2740	T4043	T4044	advmod	a,qualitative
R2741	T4044	T4045	amod	qualitative,effect
R2742	T4045	T4042	dobj	effect,suggesting
R2743	T4046	T4047	advmod	rather,than
R2744	T4047	T4044	cc	than,qualitative
R2745	T4048	T4049	advmod	a,quantitative
R2746	T4049	T4044	conj	quantitative,qualitative
R2747	T4050	T4045	prep	on,effect
R2748	T4051	T4052	det	these,phenotypes
R2749	T4052	T4050	pobj	phenotypes,on
R2750	T4053	T4054	advmod	in,vivo
R2751	T4054	T4045	advmod	vivo,effect
R2752	T4055	T4007	punct	.,were
R2753	T4057	T4058	prep	In,affect
R2754	T4059	T4057	pobj	contrast,In
R2755	T4060	T4058	punct	", ",affect
R2756	T4061	T4062	det	the,level
R2757	T4062	T4058	nsubj	level,affect
R2758	T4063	T4062	prep	of,level
R2759	T4064	T4065	compound	XPCS,expression
R2760	T4065	T4063	pobj	expression,of
R2761	T4066	T4065	compound	mRNA,expression
R2762	T4067	T4058	aux	did,affect
R2763	T4068	T4069	det	the,ability
R2764	T4069	T4058	dobj	ability,affect
R2765	T4070	T4069	prep	of,ability
R2766	T4071	T4072	det	the,protein
R2767	T4072	T4070	pobj	protein,of
R2768	T4073	T4072	amod	encoded,protein
R2769	T4074	T4072	punct	(,protein
R2770	T4075	T4072	appos	XPDG602D,protein
R2771	T4076	T4069	punct	),ability
R2772	T4077	T4078	aux	to,restore
R2773	T4078	T4069	acl	restore,ability
R2774	T4079	T4080	det	the,phenotype
R2775	T4080	T4078	dobj	phenotype,restore
R2776	T4081	T4080	compound	TTD,phenotype
R2777	T4082	T4080	compound	hair,phenotype
R2778	T4083	T4078	prep	to,restore
R2779	T4084	T4083	amod	normal,to
R2780	T4085	T4058	punct	.,affect
R2781	T4087	T4088	advmod	Notably,had
R2782	T4089	T4088	punct	", ",had
R2783	T4090	T4091	compound	XpdTTD, †XPCS
R2784	T4091	T4093	compound	†XPCS,animals
R2785	T4092	T4091	punct	/, †XPCS
R2786	T4093	T4088	nsubj	animals,had
R2787	T4094	T4095	det	a,phenotype
R2788	T4095	T4088	dobj	phenotype,had
R2789	T4096	T4095	amod	partial,phenotype
R2790	T4097	T4095	compound	TTD,phenotype
R2791	T4098	T4095	compound	hair,phenotype
R2792	T4099	T4095	punct	", ",phenotype
R2793	T4100	T4095	acl	correlating,phenotype
R2794	T4101	T4100	prep	with,correlating
R2795	T4102	T4103	amod	low,levels
R2796	T4103	T4101	pobj	levels,with
R2797	T4104	T4103	prep	of,levels
R2798	T4105	T4106	compound	Xpd†XPCS,expression
R2799	T4106	T4104	pobj	expression,of
R2800	T4107	T4088	punct	", ",had
R2801	T4108	T4109	mark	whereas,had
R2802	T4109	T4088	advcl	had,had
R2803	T4110	T4111	compound	XpdTTD,XPCS
R2804	T4111	T4113	compound	XPCS,animals
R2805	T4112	T4111	punct	/,XPCS
R2806	T4113	T4109	nsubj	animals,had
R2807	T4114	T4115	compound	wt,hair
R2808	T4115	T4109	dobj	hair,had
R2809	T4116	T4115	punct	", ",hair
R2810	T4117	T4115	acl	correlating,hair
R2811	T4118	T4117	prep	with,correlating
R2812	T4119	T4120	amod	normal,levels
R2813	T4120	T4118	pobj	levels,with
R2814	T4121	T4120	compound	expression,levels
R2815	T4122	T4120	prep	from,levels
R2816	T4123	T4124	det	the,allele
R2817	T4124	T4122	pobj	allele,from
R2818	T4125	T4124	amod	viable,allele
R2819	T4126	T4124	compound	XpdXPCS,allele
R2820	T4127	T4128	punct	(,Table
R2821	T4128	T4088	parataxis	Table,had
R2822	T4129	T4128	nummod	2,Table
R2823	T4130	T4128	cc	and,Table
R2824	T4131	T4132	amod	unpublished,data
R2825	T4132	T4128	conj	data,Table
R2826	T4133	T4128	punct	),Table
R2827	T4134	T4088	punct	.,had
R2828	T4136	T4137	advmod	Thus,affected
R2829	T4138	T4137	punct	", ",affected
R2830	T4139	T4140	det	the,range
R2831	T4140	T4137	nsubj	range,affected
R2832	T4141	T4140	prep	of,range
R2833	T4142	T4143	compound	expression,levels
R2834	T4143	T4141	pobj	levels,of
R2835	T4144	T4140	prep	from,range
R2836	T4145	T4146	det	these,alleles
R2837	T4146	T4144	pobj	alleles,from
R2838	T4147	T4146	nummod	two,alleles
R2839	T4148	T4146	amod	mutant,alleles
R2840	T4149	T4150	poss	their,ability
R2841	T4150	T4137	dobj	ability,affected
R2842	T4151	T4152	aux	to,complement
R2843	T4152	T4150	acl	complement,ability
R2844	T4153	T4154	det	some,phenotypes
R2845	T4154	T4152	dobj	phenotypes,complement
R2846	T4155	T4156	punct	(,hair
R2847	T4156	T4154	parataxis	hair,phenotypes
R2848	T4157	T4156	punct	),hair
R2849	T4158	T4137	punct	.,affected
R2850	T4160	T4161	det	An,overview
R2851	T4161	T4162	nsubjpass	overview,presented
R2852	T4163	T4161	prep	of,overview
R2853	T4164	T4165	det	the,relationships
R2854	T4165	T4163	pobj	relationships,of
R2855	T4166	T4165	amod	functional,relationships
R2856	T4167	T4165	prep	between,relationships
R2857	T4168	T4169	compound	Xpd,alleles
R2858	T4169	T4167	pobj	alleles,between
R2859	T4170	T4169	punct	", ",alleles
R2860	T4171	T4169	conj	phenotypes,alleles
R2861	T4172	T4171	punct	", ",phenotypes
R2862	T4173	T4171	cc	and,phenotypes
R2863	T4174	T4175	det	the,function
R2864	T4175	T4171	conj	function,phenotypes
R2865	T4176	T4175	amod	presumed,function
R2866	T4177	T4175	amod	underlying,function
R2867	T4178	T4175	compound	TFIIH,function
R2868	T4179	T4165	prep	in,relationships
R2869	T4180	T4179	pobj	mice,in
R2870	T4181	T4180	cc	and,mice
R2871	T4182	T4180	conj	cells,mice
R2872	T4183	T4162	auxpass	is,presented
R2873	T4184	T4162	prep	in,presented
R2874	T4185	T4184	pobj	Table,in
R2875	T4186	T4185	nummod	2,Table
R2876	T4187	T4162	punct	.,presented
R4726	T7096	T7095	prep	of,Targeting
R4727	T7097	T7098	det	the,Gene
R4728	T7098	T7096	pobj	Gene,of
R4729	T7099	T7098	compound	Mouse,Gene
R4730	T7100	T7098	compound	Xpd,Gene
R4731	T7102	T7103	punct	(,A
R4732	T7103	T7104	meta	A,representation
R4733	T7105	T7103	punct	),A
R4734	T7106	T7104	amod	Schematic,representation
R4735	T7107	T7104	prep	of,representation
R4736	T7108	T7109	det	the,structure
R4737	T7109	T7107	pobj	structure,of
R4738	T7110	T7109	amod	genomic,structure
R4739	T7111	T7104	cc	and,representation
R4740	T7112	T7113	amod	partial,map
R4741	T7113	T7104	conj	map,representation
R4742	T7114	T7113	compound	restriction,map
R4743	T7115	T7113	prep	of,map
R4744	T7116	T7117	det	the,loci
R4745	T7117	T7115	pobj	loci,of
R4746	T7118	T7117	nmod	wt,loci
R4747	T7119	T7118	cc	and,wt
R4748	T7120	T7118	conj	targeted,wt
R4749	T7121	T7117	compound	mouse,loci
R4750	T7122	T7117	compound	Xpd,loci
R4751	T7123	T7113	punct	.,map
R4752	T7125	T7126	prep	For,represented
R4753	T7127	T7128	det	the,allele
R4754	T7128	T7125	pobj	allele,For
R4755	T7129	T7128	compound	wt,allele
R4756	T7130	T7128	compound	Xpd,allele
R4757	T7131	T7126	punct	", ",represented
R4758	T7132	T7133	amod	shaded,boxes
R4759	T7133	T7134	nsubj	boxes,represent
R4760	T7134	T7126	ccomp	represent,represented
R4761	T7135	T7136	amod	coding,regions
R4762	T7136	T7134	dobj	regions,represent
R4763	T7137	T7136	prep	of,regions
R4764	T7138	T7139	nmod	exons,12
R4765	T7139	T7137	pobj	12,of
R4766	T7140	T7139	cc	and,12
R4767	T7141	T7139	conj	19,12
R4768	T7142	T7143	punct	–,23
R4769	T7143	T7141	prep	23,19
R4770	T7144	T7126	punct	;,represented
R4771	T7145	T7146	det	the,UTR
R4772	T7146	T7126	nsubjpass	UTR,represented
R4773	T7147	T7146	nummod	3,UTR
R4774	T7148	T7147	punct	′,3
R4775	T7149	T7126	auxpass	is,represented
R4776	T7150	T7126	agent	by,represented
R4777	T7151	T7152	det	an,box
R4778	T7152	T7150	pobj	box,by
R4779	T7153	T7152	amod	open,box
R4780	T7154	T7126	punct	.,represented
R4781	T7156	T7157	nsubj	TGA,indicates
R4782	T7157	T7158	ccomp	indicates,indicates
R4783	T7159	T7160	det	the,codon
R4784	T7160	T7157	dobj	codon,indicates
R4785	T7161	T7160	amod	translational,codon
R4786	T7162	T7160	compound	stop,codon
R4787	T7163	T7158	punct	;,indicates
R4788	T7164	T7158	nsubj	PolyA,indicates
R4789	T7165	T7166	det	the,signal
R4790	T7166	T7158	dobj	signal,indicates
R4791	T7167	T7166	compound	polyadenylation,signal
R4792	T7168	T7158	punct	.,indicates
R4793	T7170	T7171	prep	For,indicated
R4794	T7172	T7173	det	the,allele
R4795	T7173	T7170	pobj	allele,For
R4796	T7174	T7173	nmod	XpdTTD,allele
R4797	T7175	T7173	amod	targeted,allele
R4798	T7176	T7171	punct	", ",indicated
R4799	T7177	T7178	det	the,fragment
R4800	T7178	T7171	nsubjpass	fragment,indicated
R4801	T7179	T7180	nummod	194,pair
R4802	T7180	T7178	nmod	pair,fragment
R4803	T7181	T7180	punct	–,pair
R4804	T7182	T7180	nmod	base,pair
R4805	T7183	T7180	punct	(,pair
R4806	T7184	T7180	appos	bp,pair
R4807	T7185	T7178	punct	),fragment
R4808	T7186	T7178	amod	human,fragment
R4809	T7187	T7178	compound	XPD,fragment
R4810	T7188	T7178	compound	cDNA,fragment
R4811	T7189	T7178	acl	fused,fragment
R4812	T7190	T7189	prep	to,fused
R4813	T7191	T7190	pobj	exon,to
R4814	T7192	T7191	nummod	22,exon
R4815	T7193	T7171	auxpass	is,indicated
R4816	T7194	T7171	prep	as,indicated
R4817	T7195	T7196	det	a,box
R4818	T7196	T7194	pobj	box,as
R4819	T7197	T7196	amod	striped,box
R4820	T7198	T7171	prep	including,indicated
R4821	T7199	T7200	det	the,mutation
R4822	T7200	T7198	pobj	mutation,including
R4823	T7201	T7200	nmod	TTD,mutation
R4824	T7202	T7203	punct	(,R722W
R4825	T7203	T7201	parataxis	R722W,TTD
R4826	T7204	T7203	punct	),R722W
R4827	T7205	T7200	acl	indicated,mutation
R4828	T7206	T7205	agent	by,indicated
R4829	T7207	T7208	det	a,arrow
R4830	T7208	T7206	pobj	arrow,by
R4831	T7209	T7208	amod	vertical,arrow
R4832	T7210	T7171	punct	.,indicated
R4833	T7212	T7213	nmod	Chicken,2
R4834	T7213	T7218	nsubjpass	2,indicated
R4835	T7214	T7215	nmod	β,globin
R4836	T7215	T7213	nmod	globin,2
R4837	T7216	T7215	punct	-,globin
R4838	T7217	T7213	nmod	exons,2
R4839	T7219	T7213	cc	and,2
R4840	T7220	T7213	conj	3,2
R4841	T7221	T7213	prep	including,2
R4842	T7222	T7223	det	the,UTR
R4843	T7223	T7221	pobj	UTR,including
R4844	T7224	T7223	nummod	3,UTR
R4845	T7225	T7224	punct	′,3
R4846	T7226	T7218	auxpass	are,indicated
R4847	T7227	T7218	prep	as,indicated
R4848	T7228	T7229	amod	black,boxes
R4849	T7229	T7227	pobj	boxes,as
R4850	T7230	T7229	prep	with,boxes
R4851	T7231	T7232	amod	corresponding,numerals
R4852	T7232	T7230	pobj	numerals,with
R4853	T7233	T7232	compound	Roman,numerals
R4854	T7234	T7232	acl	followed,numerals
R4855	T7235	T7234	agent	by,followed
R4856	T7236	T7237	det	the,signal
R4857	T7237	T7235	pobj	signal,by
R4858	T7238	T7239	compound	β,globin
R4859	T7239	T7237	compound	globin,signal
R4860	T7240	T7239	punct	-,globin
R4861	T7241	T7237	compound	polyadenylation,signal
R4862	T7242	T7243	punct	(,PolyA*
R4863	T7243	T7237	parataxis	PolyA*,signal
R4864	T7244	T7243	punct	),PolyA*
R4865	T7245	T7218	punct	.,indicated
R4866	T7247	T7248	prep	For,indicate
R4867	T7249	T7250	det	the,alleles
R4868	T7250	T7247	pobj	alleles,For
R4869	T7251	T7250	nmod	Xpd†XP,alleles
R4870	T7252	T7251	cc	and,Xpd†XP
R4871	T7253	T7251	conj	Xpd†XPCS,Xpd†XP
R4872	T7254	T7250	amod	targeted,alleles
R4873	T7255	T7248	punct	", ",indicate
R4874	T7256	T7257	amod	vertical,arrows
R4875	T7257	T7248	nsubj	arrows,indicate
R4876	T7258	T7259	nmod	XPCS,mutations
R4877	T7259	T7248	dobj	mutations,indicate
R4878	T7260	T7261	punct	(,encoding
R4879	T7261	T7258	parataxis	encoding,XPCS
R4880	T7262	T7261	npadvmod	G602D,encoding
R4881	T7263	T7261	punct	-,encoding
R4882	T7264	T7261	punct	),encoding
R4883	T7265	T7258	cc	and,XPCS
R4884	T7266	T7258	conj	XP,XPCS
R4885	T7267	T7268	punct	(,encoding
R4886	T7268	T7266	parataxis	encoding,XP
R4887	T7269	T7268	npadvmod	R683W,encoding
R4888	T7270	T7268	punct	-,encoding
R4889	T7271	T7268	punct	),encoding
R4890	T7272	T7259	prep	in,mutations
R4891	T7273	T7274	nmod	exons,19
R4892	T7274	T7272	pobj	19,in
R4893	T7275	T7274	cc	and,19
R4894	T7276	T7274	conj	22,19
R4895	T7277	T7248	punct	", ",indicate
R4896	T7278	T7248	advmod	respectively,indicate
R4897	T7279	T7248	punct	.,indicate
R4898	T7281	T7282	det	The,probe
R4899	T7282	T7286	nsubjpass	probe,marked
R4900	T7283	T7282	amod	unique,probe
R4901	T7284	T7282	nummod	3,probe
R4902	T7285	T7284	punct	′,3
R4903	T7287	T7282	acl	located,probe
R4904	T7288	T7287	prep	outside,located
R4905	T7289	T7290	det	the,construct
R4906	T7290	T7288	pobj	construct,outside
R4907	T7291	T7290	amod	targeting,construct
R4908	T7292	T7286	auxpass	is,marked
R4909	T7293	T7286	agent	by,marked
R4910	T7294	T7295	det	a,line
R4911	T7295	T7293	pobj	line,by
R4912	T7296	T7295	amod	thick,line
R4913	T7297	T7295	amod	black,line
R4914	T7298	T7286	punct	.,marked
R4915	T7300	T7301	compound	Restriction,sites
R4916	T7302	T7301	punct	: ,sites
R4917	T7303	T7301	appos	B,sites
R4918	T7304	T7303	punct	", ",B
R4919	T7305	T7303	appos	BamHI,B
R4920	T7306	T7303	punct	;,B
R4921	T7307	T7303	appos	C,B
R4922	T7308	T7307	punct	", ",C
R4923	T7309	T7307	appos	ClaI,C
R4924	T7310	T7303	punct	;,B
R4925	T7311	T7303	appos	E,B
R4926	T7312	T7311	punct	", ",E
R4927	T7313	T7311	appos	EcoRI,E
R4928	T7314	T7303	punct	;,B
R4929	T7315	T7303	appos	H,B
R4930	T7316	T7315	punct	", ",H
R4931	T7317	T7315	appos	HindIII,H
R4932	T7318	T7303	punct	;,B
R4933	T7319	T7303	appos	Hp,B
R4934	T7320	T7319	punct	", ",Hp
R4935	T7321	T7319	appos	HpaI,Hp
R4936	T7322	T7303	punct	;,B
R4937	T7323	T7303	appos	Sf,B
R4938	T7324	T7323	punct	", ",Sf
R4939	T7325	T7323	appos	SfiI,Sf
R4940	T7326	T7301	punct	.,sites
R4941	T7328	T7329	punct	(,B
R4942	T7329	T7330	meta	B,analysis
R4943	T7331	T7329	punct	),B
R4944	T7332	T7333	compound	Southern,blot
R4945	T7333	T7330	compound	blot,analysis
R4946	T7334	T7330	prep	of,analysis
R4947	T7335	T7336	npadvmod	EcoRI,digested
R4948	T7336	T7338	amod	digested,DNA
R4949	T7337	T7336	punct	-,digested
R4950	T7338	T7334	pobj	DNA,of
R4951	T7339	T7338	amod	genomic,DNA
R4952	T7340	T7338	prep	from,DNA
R4953	T7341	T7342	nmod	wt,clones
R4954	T7342	T7340	pobj	clones,from
R4955	T7343	T7341	punct	", ",wt
R4956	T7344	T7345	compound	Xpd†XPCS,wt
R4957	T7345	T7341	conj	wt,wt
R4958	T7346	T7345	punct	/,wt
R4959	T7347	T7345	punct	", ",wt
R4960	T7348	T7345	cc	and,wt
R4961	T7349	T7350	compound	Xpd†XP,wt
R4962	T7350	T7345	conj	wt,wt
R4963	T7351	T7350	punct	/,wt
R4964	T7352	T7342	amod	recombinant,clones
R4965	T7353	T7354	amod	embryonic,cell
R4966	T7354	T7342	compound	cell,clones
R4967	T7355	T7354	compound	stem,cell
R4968	T7356	T7342	acl	hybridised,clones
R4969	T7357	T7356	prep	with,hybridised
R4970	T7358	T7359	det	the,probe
R4971	T7359	T7357	pobj	probe,with
R4972	T7360	T7359	nummod	3,probe
R4973	T7361	T7360	punct	′,3
R4974	T7362	T7359	acl	depicted,probe
R4975	T7363	T7362	prep	in,depicted
R4976	T7364	T7363	punct	(,in
R4977	T7365	T7363	pobj	A,in
R4978	T7366	T7330	punct	),analysis
R4979	T7367	T7330	punct	.,analysis
R4980	T7369	T7370	det	The,allele
R4981	T7370	T7372	nsubj	allele,yields
R4982	T7371	T7370	compound	wt,allele
R4983	T7373	T7374	det	a,fragment
R4984	T7374	T7372	dobj	fragment,yields
R4985	T7375	T7376	nummod	6.5,kilobase
R4986	T7376	T7374	nmod	kilobase,fragment
R4987	T7377	T7376	punct	-,kilobase
R4988	T7378	T7376	punct	(,kilobase
R4989	T7379	T7376	appos	kb,kilobase
R4990	T7380	T7374	punct	),fragment
R4991	T7381	T7372	punct	", ",yields
R4992	T7382	T7383	mark	whereas,yield
R4993	T7383	T7372	advcl	yield,yields
R4994	T7384	T7385	preconj	both,alleles
R4995	T7385	T7383	nsubj	alleles,yield
R4996	T7386	T7385	amod	targeted,alleles
R4997	T7387	T7385	nmod	Xpd†XP,alleles
R4998	T7388	T7387	cc	and,Xpd†XP
R4999	T7389	T7387	conj	Xpd†XPCS,Xpd†XP
R5000	T7390	T7391	det	a,fragment
R5001	T7391	T7383	dobj	fragment,yield
R5002	T7392	T7393	nummod	5.1,kb
R5003	T7393	T7391	compound	kb,fragment
R5004	T7394	T7393	punct	-,kb
R5005	T7395	T7372	punct	.,yields
R5006	T7397	T7398	punct	(,C
R5007	T7398	T7399	meta	C,Genotyping
R5008	T7400	T7398	punct	),C
R5009	T7401	T7399	prep	of,Genotyping
R5010	T7402	T7403	nmod	wt,alleles
R5011	T7403	T7401	pobj	alleles,of
R5012	T7404	T7402	cc	and,wt
R5013	T7405	T7402	conj	targeted,wt
R5014	T7406	T7399	prep	by,Genotyping
R5015	T7407	T7406	pobj	PCR,by
R5016	T7408	T7399	acl	using,Genotyping
R5017	T7409	T7408	dobj	primers,using
R5018	T7410	T7409	appos	F2,primers
R5019	T7411	T7410	punct	", ",F2
R5020	T7412	T7410	conj	R1,F2
R5021	T7413	T7412	punct	", ",R1
R5022	T7414	T7412	cc	and,R1
R5023	T7415	T7412	conj	mR,R1
R5024	T7416	T7417	mark	as,indicated
R5025	T7417	T7408	advcl	indicated,using
R5026	T7418	T7408	prep	in,using
R5027	T7419	T7420	punct	(,A
R5028	T7420	T7421	meta	A,fragments
R5029	T7421	T7418	pobj	fragments,in
R5030	T7422	T7420	punct	),A
R5031	T7423	T7421	nmod	yields,fragments
R5032	T7424	T7421	prep	of,fragments
R5033	T7425	T7426	nummod	399,bp
R5034	T7426	T7424	pobj	bp,of
R5035	T7427	T7426	cc	and,bp
R5036	T7428	T7429	nummod	468,bp
R5037	T7429	T7426	conj	bp,bp
R5038	T7430	T7408	punct	", ",using
R5039	T7431	T7408	advmod	respectively,using
R5040	T7432	T7399	punct	.,Genotyping
R5041	T7434	T7435	punct	(,D
R5042	T7435	T7436	meta	D,results
R5043	T7437	T7435	punct	),D
R5044	T7438	T7439	compound	RT,PCR
R5045	T7439	T7441	compound	PCR,detection
R5046	T7440	T7439	punct	-,PCR
R5047	T7441	T7436	nsubj	detection,results
R5048	T7442	T7441	prep	of,detection
R5049	T7443	T7444	compound	mRNA,expression
R5050	T7444	T7442	pobj	expression,of
R5051	T7445	T7444	acl	originating,expression
R5052	T7446	T7445	prep	from,originating
R5053	T7447	T7448	det	the,alleles
R5054	T7448	T7446	pobj	alleles,from
R5055	T7449	T7448	amod	targeted,alleles
R5056	T7450	T7448	nmod	†XP,alleles
R5057	T7451	T7450	cc	and,†XP
R5058	T7452	T7450	conj	†XPCS,†XP
R5059	T7453	T7441	prep	in,detection
R5060	T7454	T7455	amod	embryonic,cell
R5061	T7455	T7457	compound	cell,clones
R5062	T7456	T7455	compound	stem,cell
R5063	T7457	T7453	pobj	clones,in
R5064	T7458	T7441	acl	using,detection
R5065	T7459	T7458	dobj	primers,using
R5066	T7460	T7459	appos	F1,primers
R5067	T7461	T7462	punct	(,hybridising
R5068	T7462	T7460	parataxis	hybridising,F1
R5069	T7463	T7462	prep	outside,hybridising
R5070	T7464	T7465	det	the,construct
R5071	T7465	T7463	pobj	construct,outside
R5072	T7466	T7465	amod	targeting,construct
R5073	T7467	T7462	punct	),hybridising
R5074	T7468	T7460	cc	and,F1
R5075	T7469	T7460	conj	mR,F1
R5076	T7470	T7471	mark	as,indicated
R5077	T7471	T7458	advcl	indicated,using
R5078	T7472	T7471	prep	in,indicated
R5079	T7473	T7472	punct	(,in
R5080	T7474	T7472	pobj	A,in
R5081	T7475	T7436	punct	),results
R5082	T7476	T7436	prep	in,results
R5083	T7477	T7478	det	a,fragment
R5084	T7478	T7476	pobj	fragment,in
R5085	T7479	T7480	nummod	"1,416",bp
R5086	T7480	T7478	compound	bp,fragment
R5087	T7481	T7480	punct	-,bp
R5088	T7482	T7436	punct	.,results
R5089	T7484	T7485	punct	(,E
R5090	T7485	T7486	meta	E,analysis
R5091	T7487	T7485	punct	),E
R5092	T7488	T7489	compound	Northern,blot
R5093	T7489	T7486	compound	blot,analysis
R5094	T7490	T7486	prep	of,analysis
R5095	T7491	T7492	amod	total,RNA
R5096	T7492	T7490	pobj	RNA,of
R5097	T7493	T7492	acl	isolated,RNA
R5098	T7494	T7493	prep	from,isolated
R5099	T7495	T7494	pobj	testis,from
R5100	T7496	T7495	prep	of,testis
R5101	T7497	T7498	amod	homozygous,wt
R5102	T7498	T7499	nmod	wt,mice
R5103	T7499	T7496	pobj	mice,of
R5104	T7500	T7498	cc	and,wt
R5105	T7501	T7502	compound	XpdTTD,TTD
R5106	T7502	T7498	conj	TTD,wt
R5107	T7503	T7502	punct	/,TTD
R5108	T7504	T7498	punct	", ",wt
R5109	T7505	T7506	amod	heterozygous,wt
R5110	T7506	T7498	conj	wt,wt
R5111	T7507	T7506	compound	Xpd†XPCS,wt
R5112	T7508	T7506	punct	/,wt
R5113	T7509	T7506	cc	and,wt
R5114	T7510	T7511	compound	XpdTTD,wt
R5115	T7511	T7506	conj	wt,wt
R5116	T7512	T7511	punct	/,wt
R5117	T7513	T7506	punct	", ",wt
R5118	T7514	T7506	cc	and,wt
R5119	T7515	T7516	amod	compound,TTD
R5120	T7516	T7506	conj	TTD,wt
R5121	T7517	T7516	amod	heterozygous,TTD
R5122	T7518	T7516	compound	Xpd†XPCS,TTD
R5123	T7519	T7516	punct	/,TTD
R5124	T7520	T7521	mark	as,indicated
R5125	T7521	T7493	advcl	indicated,isolated
R5126	T7522	T7486	punct	.,analysis
R5127	T7524	T7525	nsubj	Hybridisation,detects
R5128	T7526	T7524	prep	with,Hybridisation
R5129	T7527	T7528	det	a,probe
R5130	T7528	T7526	pobj	probe,with
R5131	T7529	T7530	nummod	1.4,kb
R5132	T7530	T7528	compound	kb,probe
R5133	T7531	T7530	punct	-,kb
R5134	T7532	T7528	compound	mouse,probe
R5135	T7533	T7528	compound	Xpd,probe
R5136	T7534	T7528	compound	cDNA,probe
R5137	T7535	T7525	dobj	mRNAs,detects
R5138	T7536	T7535	prep	of,mRNAs
R5139	T7537	T7538	nummod	4,kb
R5140	T7538	T7536	pobj	kb,of
R5141	T7539	T7537	punct	", ",4
R5142	T7540	T7537	conj	3.3,4
R5143	T7541	T7540	punct	", ",3.3
R5144	T7542	T7540	cc	and,3.3
R5145	T7543	T7540	conj	2.7,3.3
R5146	T7544	T7535	prep	from,mRNAs
R5147	T7545	T7546	nmod	wt,alleles
R5148	T7546	T7544	pobj	alleles,from
R5149	T7547	T7545	punct	", ",wt
R5150	T7548	T7545	conj	Xpd†XPCS,wt
R5151	T7549	T7548	punct	", ",Xpd†XPCS
R5152	T7550	T7548	cc	and,Xpd†XPCS
R5153	T7551	T7548	conj	XpdTTD,Xpd†XPCS
R5154	T7552	T7535	punct	", ",mRNAs
R5155	T7553	T7535	advmod	respectively,mRNAs
R5156	T7554	T7525	punct	.,detects
R5157	T7556	T7557	det	An,gel
R5158	T7557	T7565	nsubjpass	gel,shown
R5159	T7558	T7559	compound	ethidium,bromide
R5160	T7559	T7560	npadvmod	bromide,stained
R5161	T7560	T7557	amod	stained,gel
R5162	T7561	T7559	punct	(,bromide
R5163	T7562	T7559	appos	EtBr,bromide
R5164	T7563	T7560	punct	),stained
R5165	T7564	T7560	punct	–,stained
R5166	T7566	T7557	acl	showing,gel
R5167	T7567	T7568	det	the,amount
R5168	T7568	T7566	dobj	amount,showing
R5169	T7569	T7568	prep	of,amount
R5170	T7570	T7571	amod	total,RNA
R5171	T7571	T7569	pobj	RNA,of
R5172	T7572	T7571	acl	loaded,RNA
R5173	T7573	T7565	auxpass	is,shown
R5174	T7574	T7565	advmod	below,shown
R5175	T7575	T7565	punct	.,shown
R5176	T7658	T7659	amod	Partial,Rescue
R5177	T7660	T7659	prep	of,Rescue
R5178	T7661	T7660	pobj	TTD,of
R5179	T7662	T7661	amod	Cutaneous,TTD
R5180	T7663	T7661	punct	", ",TTD
R5181	T7664	T7661	conj	Blood,TTD
R5182	T7665	T7664	punct	", ",Blood
R5183	T7666	T7664	cc	and,Blood
R5184	T7667	T7668	amod	Developmental,Phenotypes
R5185	T7668	T7664	conj	Phenotypes,Blood
R5186	T7669	T7659	prep	in,Rescue
R5187	T7670	T7671	nmod	Compound,Mice
R5188	T7671	T7669	pobj	Mice,in
R5189	T7672	T7671	amod	Heterozygous,Mice
R5190	T7673	T7674	compound	XpdTTD,†XPCS
R5191	T7674	T7671	compound	†XPCS,Mice
R5192	T7675	T7674	punct	/,†XPCS
R5193	T7677	T7678	punct	(,A
R5194	T7678	T7679	meta	A,Photographs
R5195	T7680	T7678	punct	),A
R5196	T7681	T7679	prep	of,Photographs
R5197	T7682	T7683	nummod	5,mo
R5198	T7683	T7685	npadvmod	mo,old
R5199	T7684	T7683	punct	-,mo
R5200	T7685	T7687	amod	old,mice
R5201	T7686	T7685	punct	-,old
R5202	T7687	T7681	pobj	mice,of
R5203	T7688	T7689	amod	homozygous,TTD
R5204	T7689	T7687	nmod	TTD,mice
R5205	T7690	T7689	nmod	XpdTTD,TTD
R5206	T7691	T7689	punct	/,TTD
R5207	T7692	T7689	punct	", ",TTD
R5208	T7693	T7694	nmod	compound,†XPCS
R5209	T7694	T7689	conj	†XPCS,TTD
R5210	T7695	T7694	amod	heterozygous,†XPCS
R5211	T7696	T7694	compound	XpdTTD,†XPCS
R5212	T7697	T7694	punct	/,†XPCS
R5213	T7698	T7694	punct	", ",†XPCS
R5214	T7699	T7694	cc	and,†XPCS
R5215	T7700	T7694	conj	wt,†XPCS
R5216	T7701	T7679	punct	.,Photographs
R5217	T7704	T7703	punct	: ,Insets
R5218	T7705	T7703	appos	images,Insets
R5219	T7706	T7705	prep	of,images
R5220	T7707	T7708	amod	first,round
R5221	T7708	T7710	compound	round,loss
R5222	T7709	T7708	punct	-,round
R5223	T7710	T7706	pobj	loss,of
R5224	T7711	T7710	compound	hair,loss
R5225	T7712	T7703	punct	.,Insets
R5226	T7714	T7715	punct	(,B
R5227	T7715	T7716	meta	B,analysis
R5228	T7717	T7715	punct	),B
R5229	T7718	T7716	amod	Histological,analysis
R5230	T7719	T7716	prep	of,analysis
R5231	T7720	T7721	det	the,skin
R5232	T7721	T7719	pobj	skin,of
R5233	T7722	T7721	prep	of,skin
R5234	T7723	T7724	nmod	XpdTTD,TTD
R5235	T7724	T7726	nmod	TTD,mice
R5236	T7725	T7724	punct	/,TTD
R5237	T7726	T7722	pobj	mice,of
R5238	T7727	T7724	punct	", ",TTD
R5239	T7728	T7729	compound	XpdTTD,†XPCS
R5240	T7729	T7724	conj	†XPCS,TTD
R5241	T7730	T7729	punct	/,†XPCS
R5242	T7731	T7729	punct	", ",†XPCS
R5243	T7732	T7729	cc	and,†XPCS
R5244	T7733	T7729	conj	wt,†XPCS
R5245	T7734	T7716	punct	.,analysis
R5246	T7736	T7737	npadvmod	TTD,associated
R5247	T7737	T7739	amod	associated,acanthosis
R5248	T7738	T7737	punct	-,associated
R5249	T7739	T7740	nsubj	acanthosis,were
R5250	T7741	T7742	punct	(,epidermis
R5251	T7742	T7739	parataxis	epidermis,acanthosis
R5252	T7743	T7742	amod	thicker,epidermis
R5253	T7744	T7742	punct	", ",epidermis
R5254	T7745	T7742	acl	indicated,epidermis
R5255	T7746	T7745	agent	by,indicated
R5256	T7747	T7748	amod	solid,line
R5257	T7748	T7746	pobj	line,by
R5258	T7749	T7748	amod	vertical,line
R5259	T7750	T7742	punct	),epidermis
R5260	T7751	T7739	punct	", ",acanthosis
R5261	T7752	T7753	amod	pronounced,layer
R5262	T7753	T7739	conj	layer,acanthosis
R5263	T7754	T7753	amod	granular,layer
R5264	T7755	T7753	punct	(,layer
R5265	T7756	T7753	acl	indicated,layer
R5266	T7757	T7756	agent	by,indicated
R5267	T7758	T7757	pobj	arrows,by
R5268	T7759	T7753	punct	),layer
R5269	T7760	T7753	punct	", ",layer
R5270	T7761	T7753	cc	and,layer
R5271	T7762	T7763	amod	sebacious,hyperplasia
R5272	T7763	T7753	conj	hyperplasia,layer
R5273	T7764	T7763	compound	gland,hyperplasia
R5274	T7765	T7763	punct	(,hyperplasia
R5275	T7766	T7763	acl	indicated,hyperplasia
R5276	T7767	T7766	agent	by,indicated
R5277	T7768	T7769	amod	dotted,line
R5278	T7769	T7767	pobj	line,by
R5279	T7770	T7769	amod	vertical,line
R5280	T7771	T7740	punct	),were
R5281	T7772	T7740	acomp	absent,were
R5282	T7773	T7740	prep	in,were
R5283	T7774	T7775	det	the,epidermis
R5284	T7775	T7773	pobj	epidermis,in
R5285	T7776	T7775	prep	of,epidermis
R5286	T7777	T7778	nmod	XpdTTD,†XPCS
R5287	T7778	T7780	nmod	†XPCS,mice
R5288	T7779	T7778	punct	/,†XPCS
R5289	T7780	T7776	pobj	mice,of
R5290	T7781	T7778	cc	and,†XPCS
R5291	T7782	T7778	conj	wt,†XPCS
R5292	T7783	T7740	punct	.,were
R5293	T7786	T7785	appos	400,Magnification
R5294	T7787	T7786	punct	×,400
R5295	T7788	T7785	punct	.,Magnification
R5296	T7790	T7791	punct	(,C
R5297	T7791	T7792	meta	C,content
R5298	T7793	T7791	punct	),C
R5299	T7794	T7792	compound	Cysteine,content
R5300	T7795	T7792	prep	of,content
R5301	T7796	T7795	pobj	hair,of
R5302	T7797	T7796	prep	from,hair
R5303	T7798	T7799	nmod	wt,mice
R5304	T7799	T7797	pobj	mice,from
R5305	T7800	T7798	punct	", ",wt
R5306	T7801	T7802	compound	XpdTTD,TTD
R5307	T7802	T7798	conj	TTD,wt
R5308	T7803	T7802	punct	/,TTD
R5309	T7804	T7802	punct	", ",TTD
R5310	T7805	T7802	cc	and,TTD
R5311	T7806	T7807	compound	XpdTTD,†XPCS
R5312	T7807	T7802	conj	†XPCS,TTD
R5313	T7808	T7807	punct	/,†XPCS
R5314	T7809	T7792	punct	.,content
R5315	T7811	T7812	det	The,value
R5316	T7812	T7815	nsubj	value,indicates
R5317	T7813	T7812	compound	p,value
R5318	T7814	T7812	punct	-,value
R5319	T7816	T7817	amod	significant,differences
R5320	T7817	T7815	dobj	differences,indicates
R5321	T7818	T7817	prep	between,differences
R5322	T7819	T7818	pobj	mutants,between
R5323	T7820	T7819	cc	and,mutants
R5324	T7821	T7819	conj	wt,mutants
R5325	T7822	T7818	punct	", ",between
R5326	T7823	T7824	advmod	as,as
R5327	T7824	T7818	cc	as,between
R5328	T7825	T7824	advmod	well,as
R5329	T7826	T7818	conj	between,between
R5330	T7827	T7828	nmod	XpdTTD,TTD
R5331	T7828	T7830	nmod	TTD,mice
R5332	T7829	T7828	punct	/,TTD
R5333	T7830	T7826	pobj	mice,between
R5334	T7831	T7828	cc	and,TTD
R5335	T7832	T7833	compound	XpdTTD,†XPCS
R5336	T7833	T7828	conj	†XPCS,TTD
R5337	T7834	T7833	punct	/,†XPCS
R5338	T7835	T7815	punct	.,indicates
R5339	T7837	T7838	compound	Error,bars
R5340	T7838	T7839	nsubj	bars,indicate
R5341	T7840	T7841	amod	standard,error
R5342	T7841	T7839	dobj	error,indicate
R5343	T7842	T7841	prep	of,error
R5344	T7843	T7844	det	the,mean
R5345	T7844	T7842	pobj	mean,of
R5346	T7845	T7841	punct	(,error
R5347	T7846	T7841	appos	SEM,error
R5348	T7847	T7839	punct	),indicate
R5349	T7848	T7839	punct	.,indicate
R5350	T7850	T7851	punct	(,D
R5351	T7851	T7852	meta	D,values
R5352	T7853	T7851	punct	),D
R5353	T7854	T7852	compound	Hematocrit,values
R5354	T7855	T7852	prep	from,values
R5355	T7856	T7855	pobj	blood,from
R5356	T7857	T7856	prep	of,blood
R5357	T7858	T7859	nmod	XpdTTD,TTD
R5358	T7859	T7861	nmod	TTD,mice
R5359	T7860	T7859	punct	/,TTD
R5360	T7861	T7857	pobj	mice,of
R5361	T7862	T7859	cc	and,TTD
R5362	T7863	T7864	compound	XpdTTD,†XPCS
R5363	T7864	T7859	conj	†XPCS,TTD
R5364	T7865	T7864	punct	/,†XPCS
R5365	T7866	T7852	punct	.,values
R5366	T7868	T7869	det	The,values
R5367	T7869	T7872	nsubj	values,indicate
R5368	T7870	T7869	compound	p,values
R5369	T7871	T7869	punct	-,values
R5370	T7873	T7874	det	the,significance
R5371	T7874	T7872	dobj	significance,indicate
R5372	T7875	T7874	prep	of,significance
R5373	T7876	T7877	det	the,difference
R5374	T7877	T7875	pobj	difference,of
R5375	T7878	T7872	advcl	relative,indicate
R5376	T7879	T7878	prep	to,relative
R5377	T7880	T7879	pobj	wt,to
R5378	T7881	T7872	punct	.,indicate
R5379	T7883	T7884	compound	Error,bars
R5380	T7884	T7885	nsubj	bars,indicate
R5381	T7886	T7885	dobj	SEM,indicate
R5382	T7887	T7885	punct	.,indicate
R5383	T7889	T7890	punct	(,E
R5384	T7890	T7891	meta	E,weights
R5385	T7892	T7890	punct	),E
R5386	T7893	T7891	compound	Body,weights
R5387	T7894	T7891	prep	of,weights
R5388	T7895	T7896	amod	developing,mice
R5389	T7896	T7894	pobj	mice,of
R5390	T7897	T7898	nmod	XpdTTD,TTD
R5391	T7898	T7896	nmod	TTD,mice
R5392	T7899	T7898	punct	/,TTD
R5393	T7900	T7898	cc	and,TTD
R5394	T7901	T7902	compound	XpdTTD,†XPCS
R5395	T7902	T7898	conj	†XPCS,TTD
R5396	T7903	T7902	punct	/,†XPCS
R5397	T7904	T7896	prep	after,mice
R5398	T7905	T7904	pobj	weaning,after
R5399	T7906	T7891	acl	plotted,weights
R5400	T7907	T7906	prep	as,plotted
R5401	T7908	T7909	det	a,percentage
R5402	T7909	T7907	pobj	percentage,as
R5403	T7910	T7909	prep	of,percentage
R5404	T7911	T7912	det	the,weight
R5405	T7912	T7910	pobj	weight,of
R5406	T7913	T7912	prep	of,weight
R5407	T7914	T7915	npadvmod	age,matched
R5408	T7915	T7917	amod	matched,littermates
R5409	T7916	T7915	punct	-,matched
R5410	T7917	T7913	pobj	littermates,of
R5411	T7918	T7917	nmod	control,littermates
R5412	T7919	T7917	nmod	wt,littermates
R5413	T7920	T7919	cc	and,wt
R5414	T7921	T7919	conj	heterozygote,wt
R5415	T7922	T7923	punct	(,hz
R5416	T7923	T7921	parataxis	hz,heterozygote
R5417	T7924	T7923	punct	),hz
R5418	T7925	T7926	punct	(,set
R5419	T7926	T7909	parataxis	set,percentage
R5420	T7927	T7926	prep	at,set
R5421	T7928	T7929	nummod	100,%
R5422	T7929	T7927	pobj	%,at
R5423	T7930	T7926	punct	),set
R5424	T7931	T7891	punct	.,weights
R5425	T7933	T7934	compound	Error,bars
R5426	T7934	T7935	nsubj	bars,indicate
R5427	T7936	T7935	dobj	SEM,indicate
R5428	T7937	T7935	punct	.,indicate
R5429	T8042	T8041	prep	of,Rescue
R5430	T8043	T8044	npadvmod	TTD,Associated
R5431	T8044	T8046	amod	Associated,Features
R5432	T8045	T8044	punct	-,Associated
R5433	T8046	T8042	pobj	Features,of
R5434	T8047	T8046	amod	Segmental,Features
R5435	T8048	T8046	amod	Progeroid,Features
R5436	T8049	T8046	prep	in,Features
R5437	T8050	T8051	amod	Compound,Mice
R5438	T8051	T8049	pobj	Mice,in
R5439	T8052	T8051	amod	Heterozygous,Mice
R5440	T8053	T8054	compound	Xpd TTD,†XPCS
R5441	T8054	T8051	compound	†XPCS,Mice
R5442	T8055	T8054	punct	/,†XPCS
R5443	T8057	T8058	punct	(,A
R5444	T8058	T8059	meta	A,Photographs
R5445	T8060	T8058	punct	),A
R5446	T8061	T8059	prep	of,Photographs
R5447	T8062	T8063	nummod	20,mo
R5448	T8063	T8065	npadvmod	mo,old
R5449	T8064	T8063	punct	-,mo
R5450	T8065	T8067	amod	old,mice
R5451	T8066	T8065	punct	-,old
R5452	T8067	T8061	pobj	mice,of
R5453	T8068	T8067	nmod	wt,mice
R5454	T8069	T8068	punct	", ",wt
R5455	T8070	T8071	amod	compound,†XPCS
R5456	T8071	T8068	conj	†XPCS,wt
R5457	T8072	T8071	amod	heterozygous,†XPCS
R5458	T8073	T8071	compound	XpdTTD,†XPCS
R5459	T8074	T8071	punct	/,†XPCS
R5460	T8075	T8071	punct	", ",†XPCS
R5461	T8076	T8071	cc	and,†XPCS
R5462	T8077	T8078	amod	homozygous,TTD
R5463	T8078	T8071	conj	TTD,†XPCS
R5464	T8079	T8078	compound	XpdTTD,TTD
R5465	T8080	T8078	punct	/,TTD
R5466	T8081	T8059	punct	.,Photographs
R5467	T8084	T8085	det	the,cachexia
R5468	T8085	T8083	dobj	cachexia,Note
R5469	T8086	T8085	amod	extreme,cachexia
R5470	T8087	T8085	punct	(,cachexia
R5471	T8088	T8085	appos	lack,cachexia
R5472	T8089	T8088	prep	of,lack
R5473	T8090	T8091	amod	subcutaneous,fat
R5474	T8091	T8089	pobj	fat,of
R5475	T8092	T8085	punct	),cachexia
R5476	T8093	T8085	prep	in,cachexia
R5477	T8094	T8095	det	the,mouse
R5478	T8095	T8093	pobj	mouse,in
R5479	T8096	T8097	compound	XpdTTD,TTD
R5480	T8097	T8095	compound	TTD,mouse
R5481	T8098	T8097	punct	/,TTD
R5482	T8099	T8083	cc	and,Note
R5483	T8100	T8101	det	the,absence
R5484	T8101	T8083	conj	absence,Note
R5485	T8102	T8101	prep	of,absence
R5486	T8103	T8104	det	this,phenotype
R5487	T8104	T8102	pobj	phenotype,of
R5488	T8105	T8101	prep	in,absence
R5489	T8106	T8107	nmod	wt,mice
R5490	T8107	T8105	pobj	mice,in
R5491	T8108	T8106	cc	and,wt
R5492	T8109	T8110	compound	XpdTTD,†XPCS
R5493	T8110	T8106	conj	†XPCS,wt
R5494	T8111	T8110	punct	/,†XPCS
R5495	T8112	T8083	punct	.,Note
R5496	T8114	T8115	punct	(,B
R5497	T8115	T8116	meta	B,Radiographs
R5498	T8117	T8115	punct	),B
R5499	T8118	T8116	prep	of,Radiographs
R5500	T8119	T8120	nummod	20,mo
R5501	T8120	T8122	npadvmod	mo,old
R5502	T8121	T8120	punct	-,mo
R5503	T8122	T8124	amod	old,mice
R5504	T8123	T8122	punct	-,old
R5505	T8124	T8118	pobj	mice,of
R5506	T8125	T8124	amod	male,mice
R5507	T8126	T8124	nmod	wt,mice
R5508	T8127	T8126	punct	", ",wt
R5509	T8128	T8129	compound	XpdTTD,†XPCS
R5510	T8129	T8126	conj	†XPCS,wt
R5511	T8130	T8129	punct	/,†XPCS
R5512	T8131	T8129	punct	", ",†XPCS
R5513	T8132	T8129	cc	and,†XPCS
R5514	T8133	T8134	compound	XpdTTD,TTD
R5515	T8134	T8129	conj	TTD,†XPCS
R5516	T8135	T8134	punct	/,TTD
R5517	T8136	T8116	punct	.,Radiographs
R5518	T8138	T8139	amod	Ageing,mice
R5519	T8139	T8143	nsubj	mice,develop
R5520	T8140	T8141	compound	XpdTTD,TTD
R5521	T8141	T8139	compound	TTD,mice
R5522	T8142	T8141	punct	/,TTD
R5523	T8144	T8143	dobj	kyphosis,develop
R5524	T8145	T8144	punct	(,kyphosis
R5525	T8146	T8144	appos	curvature,kyphosis
R5526	T8147	T8146	prep	of,curvature
R5527	T8148	T8149	det	the,column
R5528	T8149	T8147	pobj	column,of
R5529	T8150	T8149	amod	spinal,column
R5530	T8151	T8144	punct	),kyphosis
R5531	T8152	T8144	cc	and,kyphosis
R5532	T8153	T8144	conj	reduction,kyphosis
R5533	T8154	T8153	prep	of,reduction
R5534	T8155	T8156	compound	bone,mineral
R5535	T8156	T8157	compound	mineral,density
R5536	T8157	T8154	pobj	density,of
R5537	T8158	T8159	mark	as,shown
R5538	T8159	T8143	advcl	shown,develop
R5539	T8160	T8159	prep	in,shown
R5540	T8161	T8162	det	the,segment
R5541	T8162	T8160	pobj	segment,in
R5542	T8163	T8164	quantmod	6,8
R5543	T8164	T8162	nummod	8,segment
R5544	T8165	T8164	punct	–,8
R5545	T8166	T8162	prep	of,segment
R5546	T8167	T8168	det	the,vertebrae
R5547	T8168	T8166	pobj	vertebrae,of
R5548	T8169	T8168	compound	tail,vertebrae
R5549	T8170	T8162	acl	counted,segment
R5550	T8171	T8170	prep	from,counted
R5551	T8172	T8173	det	the,pelvis
R5552	T8173	T8171	pobj	pelvis,from
R5553	T8174	T8175	punct	(,see
R5554	T8175	T8143	parataxis	see,develop
R5555	T8176	T8175	dobj	close,see
R5556	T8177	T8176	punct	-,close
R5557	T8178	T8176	prt	up,close
R5558	T8179	T8176	prep	at,close
R5559	T8180	T8179	pobj	right,at
R5560	T8181	T8175	punct	),see
R5561	T8182	T8143	punct	.,develop
R5562	T8185	T8186	det	the,absence
R5563	T8186	T8184	dobj	absence,Note
R5564	T8187	T8186	prep	of,absence
R5565	T8188	T8189	det	these,features
R5566	T8189	T8187	pobj	features,of
R5567	T8190	T8186	prep	in,absence
R5568	T8191	T8192	det	the,mouse
R5569	T8192	T8190	pobj	mouse,in
R5570	T8193	T8194	compound	XpdTTD , † XPCS
R5571	T8194	T8192	compound	† XPCS,mouse
R5572	T8195	T8194	punct	/, † XPCS
R5573	T8196	T8184	punct	.,Note
R5574	T8198	T8199	punct	(,C
R5575	T8199	T8200	meta	C,Quantification
R5576	T8201	T8199	punct	),C
R5577	T8202	T8200	prep	of,Quantification
R5578	T8203	T8204	amod	relative,density
R5579	T8204	T8202	pobj	density,of
R5580	T8205	T8206	compound	bone,mineral
R5581	T8206	T8204	compound	mineral,density
R5582	T8207	T8204	prep	of,density
R5583	T8208	T8209	compound	tail,vertebrae
R5584	T8209	T8207	pobj	vertebrae,of
R5585	T8210	T8209	prep	from,vertebrae
R5586	T8211	T8212	nummod	20,mo
R5587	T8212	T8214	npadvmod	mo,old
R5588	T8213	T8212	punct	-,mo
R5589	T8214	T8216	amod	old,mice
R5590	T8215	T8214	punct	-,old
R5591	T8216	T8210	pobj	mice,from
R5592	T8217	T8216	amod	male,mice
R5593	T8218	T8216	nmod	wt,mice
R5594	T8219	T8220	punct	(,3
R5595	T8220	T8218	parataxis	3,wt
R5596	T8221	T8220	nsubj	n,3
R5597	T8222	T8220	punct	=,3
R5598	T8223	T8220	punct	),3
R5599	T8224	T8218	punct	", ",wt
R5600	T8225	T8226	compound	XpdTTD,†XPCS
R5601	T8226	T8218	conj	†XPCS,wt
R5602	T8227	T8226	punct	/,†XPCS
R5603	T8228	T8229	punct	(,4
R5604	T8229	T8226	parataxis	4,†XPCS
R5605	T8230	T8229	nsubj	n,4
R5606	T8231	T8229	punct	=,4
R5607	T8232	T8229	punct	),4
R5608	T8233	T8226	punct	", ",†XPCS
R5609	T8234	T8226	cc	and,†XPCS
R5610	T8235	T8236	compound	XpdTTD,TTD
R5611	T8236	T8226	conj	TTD,†XPCS
R5612	T8237	T8236	punct	/,TTD
R5613	T8238	T8239	punct	(,3
R5614	T8239	T8236	parataxis	3,TTD
R5615	T8240	T8239	nsubj	n,3
R5616	T8241	T8239	punct	=,3
R5617	T8242	T8239	punct	),3
R5618	T8243	T8200	punct	.,Quantification
R5619	T8245	T8246	det	The,values
R5620	T8246	T8249	nsubj	values,indicate
R5621	T8247	T8246	compound	p,values
R5622	T8248	T8246	punct	-,values
R5623	T8250	T8251	det	the,significance
R5624	T8251	T8249	dobj	significance,indicate
R5625	T8252	T8251	prep	of,significance
R5626	T8253	T8254	det	the,difference
R5627	T8254	T8252	pobj	difference,of
R5628	T8255	T8249	advcl	relative,indicate
R5629	T8256	T8255	prep	to,relative
R5630	T8257	T8258	compound	XpdTTD,TTD
R5631	T8258	T8256	pobj	TTD,to
R5632	T8259	T8258	punct	/,TTD
R5633	T8260	T8249	punct	.,indicate
R5634	T8262	T8263	compound	Error,bars
R5635	T8263	T8264	nsubj	bars,indicate
R5636	T8265	T8264	dobj	SEM,indicate
R5637	T8266	T8264	punct	.,indicate
R5638	T8268	T8269	punct	(,D
R5639	T8269	T8270	meta	D,curves
R5640	T8271	T8269	punct	),D
R5641	T8272	T8273	compound	Body,weight
R5642	T8273	T8270	compound	weight,curves
R5643	T8274	T8270	prep	as,curves
R5644	T8275	T8276	det	a,function
R5645	T8276	T8274	pobj	function,as
R5646	T8277	T8276	prep	of,function
R5647	T8278	T8277	pobj	time,of
R5648	T8279	T8270	punct	.,curves
R5649	T8282	T8283	mark	that,rescued
R5650	T8283	T8281	ccomp	rescued,Note
R5651	T8284	T8285	det	the,cachexia
R5652	T8285	T8283	nsubjpass	cachexia,rescued
R5653	T8286	T8287	npadvmod	age,dependent
R5654	T8287	T8285	amod	dependent,cachexia
R5655	T8288	T8287	punct	-,dependent
R5656	T8289	T8285	acl	observed,cachexia
R5657	T8290	T8289	prep	in,observed
R5658	T8291	T8292	compound	XpdTTD,TTD
R5659	T8292	T8294	compound	TTD,mice
R5660	T8293	T8292	punct	/,TTD
R5661	T8294	T8290	pobj	mice,in
R5662	T8295	T8283	auxpass	was,rescued
R5663	T8296	T8283	prep	in,rescued
R5664	T8297	T8298	preconj	both,male
R5665	T8298	T8299	nmod	male,mice
R5666	T8299	T8296	pobj	mice,in
R5667	T8300	T8298	cc	and,male
R5668	T8301	T8298	conj	female,male
R5669	T8302	T8303	compound	XpdTTD , †XPCS
R5670	T8303	T8299	compound	†XPCS,mice
R5671	T8304	T8303	punct	/, †XPCS
R5672	T8305	T8281	punct	.,Note
R5673	T8307	T8308	amod	Significant,differences
R5674	T8308	T8309	nsubjpass	differences,observed
R5675	T8310	T8308	prep	between,differences
R5676	T8311	T8310	pobj	wt,between
R5677	T8312	T8311	cc	and,wt
R5678	T8313	T8314	compound	XpdTTD,TTD
R5679	T8314	T8311	conj	TTD,wt
R5680	T8315	T8314	punct	/,TTD
R5681	T8316	T8310	cc	but,between
R5682	T8317	T8316	neg	not,but
R5683	T8318	T8310	conj	between,between
R5684	T8319	T8320	nmod	wt,mice
R5685	T8320	T8318	pobj	mice,between
R5686	T8321	T8319	cc	and,wt
R5687	T8322	T8323	compound	XpdTTD,†XPCS
R5688	T8323	T8319	conj	†XPCS,wt
R5689	T8324	T8323	punct	/,†XPCS
R5690	T8325	T8309	auxpass	were,observed
R5691	T8326	T8309	prep	at,observed
R5692	T8327	T8328	nummod	9,mo
R5693	T8328	T8326	pobj	mo,at
R5694	T8329	T8327	cc	and,9
R5695	T8330	T8327	conj	18,9
R5696	T8331	T8328	prep	of,mo
R5697	T8332	T8331	pobj	age,of
R5698	T8333	T8334	mark	as,indicated
R5699	T8334	T8309	advcl	indicated,observed
R5700	T8335	T8334	agent	by,indicated
R5701	T8336	T8335	pobj	asterisks,by
R5702	T8337	T8309	punct	.,observed
R5703	T8339	T8340	compound	Error,bars
R5704	T8340	T8341	nsubj	bars,indicate
R5705	T8342	T8341	dobj	SEM,indicate
R5706	T8343	T8341	punct	.,indicate
R5707	T8564	T8565	compound	TFIIH,Functions
R5708	T8566	T8565	cc	and,Functions
R5709	T8567	T8565	conj	Mechanisms,Functions
R5710	T8568	T8567	prep	of,Mechanisms
R5711	T8569	T8570	npadvmod	XPD,Associated
R5712	T8570	T8572	amod	Associated,Pleiotropy
R5713	T8571	T8570	punct	-,Associated
R5714	T8572	T8568	pobj	Pleiotropy,of
R5715	T8573	T8572	compound	Disease,Pleiotropy
R5716	T8575	T8576	punct	(,A
R5717	T8576	T8577	meta	A,survival
R5718	T8578	T8576	punct	),A
R5719	T8579	T8577	amod	Cellular,survival
R5720	T8580	T8577	prep	after,survival
R5721	T8581	T8582	compound	UV,irradiation
R5722	T8582	T8580	pobj	irradiation,after
R5723	T8583	T8577	punct	.,survival
R5724	T8585	T8586	nsubjpass	Rescue,illustrated
R5725	T8587	T8585	prep	of,Rescue
R5726	T8588	T8589	amod	hemizygous,survival
R5727	T8589	T8587	pobj	survival,of
R5728	T8590	T8591	compound	XpdTTD,KO
R5729	T8591	T8589	compound	KO,survival
R5730	T8592	T8591	punct	/,KO
R5731	T8593	T8585	prep	by,Rescue
R5732	T8594	T8595	nmod	Xpd†XPCS,alleles
R5733	T8595	T8593	pobj	alleles,by
R5734	T8596	T8594	cc	and,Xpd†XPCS
R5735	T8597	T8594	conj	Xpd†XP,Xpd†XPCS
R5736	T8598	T8586	auxpass	is,illustrated
R5737	T8599	T8586	agent	by,illustrated
R5738	T8600	T8599	pobj	arrows,by
R5739	T8601	T8600	acl	marked,arrows
R5740	T8602	T8601	oprd	A,marked
R5741	T8603	T8602	cc	and,A
R5742	T8604	T8602	conj	B,A
R5743	T8605	T8601	punct	", ",marked
R5744	T8606	T8601	advmod	respectively,marked
R5745	T8607	T8586	punct	.,illustrated
R5746	T8609	T8610	compound	UV,survival
R5747	T8610	T8611	nsubjpass	survival,depicted
R5748	T8612	T8610	prep	of,survival
R5749	T8613	T8614	amod	homozygous,cells
R5750	T8614	T8612	pobj	cells,of
R5751	T8615	T8616	compound	XpdXPCS,XPCS
R5752	T8616	T8614	compound	XPCS,cells
R5753	T8617	T8616	punct	/,XPCS
R5754	T8618	T8619	punct	(,asterisk
R5755	T8619	T8614	parataxis	asterisk,cells
R5756	T8620	T8619	punct	),asterisk
R5757	T8621	T8614	prep	from,cells
R5758	T8622	T8623	det	the,allele
R5759	T8623	T8621	pobj	allele,from
R5760	T8624	T8625	advmod	normally,expressed
R5761	T8625	T8623	amod	expressed,allele
R5762	T8626	T8623	amod	viable,allele
R5763	T8627	T8628	punct	(,XpdXPCS
R5764	T8628	T8623	parataxis	XpdXPCS,allele
R5765	T8629	T8628	punct	),XpdXPCS
R5766	T8630	T8611	auxpass	is,depicted
R5767	T8631	T8611	prep	by,depicted
R5768	T8632	T8633	det	a,line
R5769	T8633	T8631	pobj	line,by
R5770	T8634	T8633	amod	dotted,line
R5771	T8635	T8611	punct	.,depicted
R5772	T8637	T8638	compound	Survival,curves
R5773	T8638	T8639	nsubj	curves,represent
R5774	T8639	T8640	ccomp	represent,included
R5775	T8641	T8642	det	an,average
R5776	T8642	T8639	dobj	average,represent
R5777	T8643	T8642	prep	of,average
R5778	T8644	T8645	nummod	four,experiments
R5779	T8645	T8643	pobj	experiments,of
R5780	T8646	T8645	amod	independent,experiments
R5781	T8647	T8640	punct	;,included
R5782	T8648	T8649	quantmod	1,2
R5783	T8649	T8651	nummod	2,lines
R5784	T8650	T8649	punct	–,2
R5785	T8651	T8640	nsubjpass	lines,included
R5786	T8652	T8651	compound	cell,lines
R5787	T8653	T8651	prep	per,lines
R5788	T8654	T8653	pobj	genotype,per
R5789	T8655	T8640	auxpass	were,included
R5790	T8656	T8640	prep	in,included
R5791	T8657	T8658	det	each,experiment
R5792	T8658	T8656	pobj	experiment,in
R5793	T8659	T8640	punct	.,included
R5794	T8661	T8662	compound	Error,bars
R5795	T8662	T8663	nsubj	bars,indicate
R5796	T8664	T8663	dobj	SEM,indicate
R5797	T8665	T8664	prep	between,SEM
R5798	T8666	T8665	pobj	experiments,between
R5799	T8667	T8663	punct	.,indicate
R5800	T8669	T8670	punct	(,B
R5801	T8670	T8671	meta	B,UDS
R5802	T8672	T8670	punct	),B
R5803	T8673	T8671	compound	UV,UDS
R5804	T8674	T8671	punct	-,UDS
R5805	T8675	T8671	punct	", ",UDS
R5806	T8676	T8677	det	a,measure
R5807	T8677	T8671	appos	measure,UDS
R5808	T8678	T8677	prep	of,measure
R5809	T8679	T8680	amod	global,repair
R5810	T8680	T8678	pobj	repair,of
R5811	T8681	T8680	compound	genome,repair
R5812	T8682	T8671	punct	.,UDS
R5813	T8684	T8685	dep	Number,included
R5814	T8686	T8684	prep	of,Number
R5815	T8687	T8686	pobj	experiments,of
R5816	T8688	T8685	punct	: ,included
R5817	T8689	T8690	nsubj	n,15
R5818	T8690	T8685	ccomp	15,included
R5819	T8691	T8690	punct	=,15
R5820	T8692	T8693	punct	(,XpdTTD
R5821	T8693	T8690	parataxis	XpdTTD,15
R5822	T8694	T8693	punct	/,XpdTTD
R5823	T8695	T8693	dep	TTD,XpdTTD
R5824	T8696	T8693	punct	),XpdTTD
R5825	T8697	T8685	punct	", ",included
R5826	T8698	T8699	nsubj	n,6
R5827	T8699	T8685	ccomp	6,included
R5828	T8700	T8699	punct	=,6
R5829	T8701	T8702	punct	(,KO
R5830	T8702	T8699	parataxis	KO,6
R5831	T8703	T8702	compound	XpdTTD,KO
R5832	T8704	T8702	punct	/,KO
R5833	T8705	T8702	punct	),KO
R5834	T8706	T8685	punct	", ",included
R5835	T8707	T8708	nsubj	n,4
R5836	T8708	T8685	ccomp	4,included
R5837	T8709	T8708	punct	=,4
R5838	T8710	T8711	punct	(,†XPCS
R5839	T8711	T8708	parataxis	†XPCS,4
R5840	T8712	T8711	compound	XpdTTD,†XPCS
R5841	T8713	T8711	punct	/,†XPCS
R5842	T8714	T8711	punct	),†XPCS
R5843	T8715	T8685	punct	", ",included
R5844	T8716	T8717	nsubj	n,2
R5845	T8717	T8685	ccomp	2,included
R5846	T8718	T8717	punct	=,2
R5847	T8719	T8720	punct	(,†XP
R5848	T8720	T8717	parataxis	†XP,2
R5849	T8721	T8720	compound	XpdTTD,†XP
R5850	T8722	T8720	punct	/,†XP
R5851	T8723	T8720	punct	),†XP
R5852	T8724	T8685	punct	;,included
R5853	T8725	T8726	quantmod	1,2
R5854	T8726	T8728	nummod	2,lines
R5855	T8727	T8726	punct	–,2
R5856	T8728	T8685	nsubjpass	lines,included
R5857	T8729	T8728	compound	cell,lines
R5858	T8730	T8728	prep	per,lines
R5859	T8731	T8730	pobj	genotype,per
R5860	T8732	T8685	auxpass	were,included
R5861	T8733	T8685	prep	in,included
R5862	T8734	T8735	det	each,experiment
R5863	T8735	T8733	pobj	experiment,in
R5864	T8736	T8685	punct	.,included
R5865	T8738	T8739	det	The,asterisk
R5866	T8739	T8740	nsubj	asterisk,indicates
R5867	T8740	T8741	ccomp	indicates,indicate
R5868	T8742	T8743	amod	significant,difference
R5869	T8743	T8740	dobj	difference,indicates
R5870	T8744	T8743	prep	with,difference
R5871	T8745	T8746	compound	XpdTTD,TTD
R5872	T8746	T8744	pobj	TTD,with
R5873	T8747	T8746	punct	/,TTD
R5874	T8748	T8741	punct	;,indicate
R5875	T8749	T8741	nsubj	crosses,indicate
R5876	T8750	T8751	amod	significant,differences
R5877	T8751	T8741	dobj	differences,indicate
R5878	T8752	T8751	prep	with,differences
R5879	T8753	T8754	compound	XpdTTD,KO
R5880	T8754	T8752	pobj	KO,with
R5881	T8755	T8754	punct	/,KO
R5882	T8756	T8741	punct	.,indicate
R5883	T8758	T8759	punct	(,C
R5884	T8759	T8760	meta	C,RRS
R5885	T8761	T8759	punct	),C
R5886	T8762	T8760	compound	UV,RRS
R5887	T8763	T8760	punct	-,RRS
R5888	T8764	T8760	punct	", ",RRS
R5889	T8765	T8766	det	a,measure
R5890	T8766	T8760	appos	measure,RRS
R5891	T8767	T8766	prep	of,measure
R5892	T8768	T8769	npadvmod	transcription,coupled
R5893	T8769	T8771	amod	coupled,repair
R5894	T8770	T8769	punct	-,coupled
R5895	T8771	T8767	pobj	repair,of
R5896	T8772	T8771	prep	of,repair
R5897	T8773	T8774	npadvmod	UV,induced
R5898	T8774	T8776	amod	induced,lesions
R5899	T8775	T8774	punct	-,induced
R5900	T8776	T8772	pobj	lesions,of
R5901	T8777	T8760	punct	.,RRS
R5902	T8779	T8780	dep	Number,included
R5903	T8781	T8779	prep	of,Number
R5904	T8782	T8781	pobj	experiments,of
R5905	T8783	T8780	punct	: ,included
R5906	T8784	T8785	nsubj	n,7
R5907	T8785	T8780	ccomp	7,included
R5908	T8786	T8785	punct	=,7
R5909	T8787	T8788	punct	(,TTD
R5910	T8788	T8785	parataxis	TTD,7
R5911	T8789	T8788	compound	XpdTTD,TTD
R5912	T8790	T8788	punct	/,TTD
R5913	T8791	T8788	punct	),TTD
R5914	T8792	T8780	punct	", ",included
R5915	T8793	T8794	nsubj	n,2
R5916	T8794	T8780	ccomp	2,included
R5917	T8795	T8794	punct	=,2
R5918	T8796	T8797	punct	(,KO
R5919	T8797	T8794	parataxis	KO,2
R5920	T8798	T8797	compound	XpdTTD,KO
R5921	T8799	T8797	punct	/,KO
R5922	T8800	T8797	punct	),KO
R5923	T8801	T8780	punct	", ",included
R5924	T8802	T8803	nsubj	n,4
R5925	T8803	T8780	ccomp	4,included
R5926	T8804	T8803	punct	=,4
R5927	T8805	T8806	punct	(,†XPCS
R5928	T8806	T8803	parataxis	†XPCS,4
R5929	T8807	T8806	compound	XpdTTD,†XPCS
R5930	T8808	T8806	punct	/,†XPCS
R5931	T8809	T8806	punct	),†XPCS
R5932	T8810	T8780	punct	", ",included
R5933	T8811	T8812	nsubj	n,2
R5934	T8812	T8780	ccomp	2,included
R5935	T8813	T8812	punct	=,2
R5936	T8814	T8815	punct	(,†XP
R5937	T8815	T8812	parataxis	†XP,2
R5938	T8816	T8815	compound	XpdTTD,†XP
R5939	T8817	T8815	punct	/,†XP
R5940	T8818	T8815	punct	),†XP
R5941	T8819	T8780	punct	;,included
R5942	T8820	T8821	quantmod	1,2
R5943	T8821	T8823	nummod	2,lines
R5944	T8822	T8821	punct	–,2
R5945	T8823	T8780	nsubjpass	lines,included
R5946	T8824	T8823	compound	cell,lines
R5947	T8825	T8823	prep	per,lines
R5948	T8826	T8825	pobj	genotype,per
R5949	T8827	T8780	auxpass	were,included
R5950	T8828	T8780	prep	in,included
R5951	T8829	T8830	det	each,experiment
R5952	T8830	T8828	pobj	experiment,in
R5953	T8831	T8780	punct	.,included
R5954	T8833	T8834	punct	(,D
R5955	T8834	T8835	meta	D,activity
R5956	T8836	T8834	punct	),D
R5957	T8837	T8838	compound	Incision,excision
R5958	T8838	T8835	compound	excision,activity
R5959	T8839	T8838	punct	/,excision
R5960	T8840	T8835	prep	of,activity
R5961	T8841	T8840	pobj	combinations,of
R5962	T8842	T8841	prep	of,combinations
R5963	T8843	T8844	amod	altered,complexes
R5964	T8844	T8842	pobj	complexes,of
R5965	T8845	T8844	compound	TFIIH,complexes
R5966	T8846	T8844	prep	in,complexes
R5967	T8847	T8848	det	a,reaction
R5968	T8848	T8846	pobj	reaction,in
R5969	T8849	T8848	amod	reconstituted,reaction
R5970	T8850	T8848	compound	NER,reaction
R5971	T8851	T8835	punct	.,activity
R5972	T8853	T8854	amod	Equal,amounts
R5973	T8854	T8855	nsubjpass	amounts,mixed
R5974	T8856	T8854	prep	of,amounts
R5975	T8857	T8858	amod	single,populations
R5976	T8858	T8856	pobj	populations,of
R5977	T8859	T8857	cc	or,single
R5978	T8860	T8857	conj	mixed,single
R5979	T8861	T8858	prep	of,populations
R5980	T8862	T8863	amod	recombinant,TFIIHs
R5981	T8863	T8861	pobj	TFIIHs,of
R5982	T8864	T8858	punct	(,populations
R5983	T8865	T8858	acl	containing,populations
R5984	T8866	T8865	dobj	XPD,containing
R5985	T8867	T8866	punct	", ",XPD
R5986	T8868	T8866	conj	XPB,XPD
R5987	T8869	T8868	punct	", ",XPB
R5988	T8870	T8868	conj	p62,XPB
R5989	T8871	T8870	punct	", ",p62
R5990	T8872	T8870	conj	p52,p62
R5991	T8873	T8872	punct	", ",p52
R5992	T8874	T8875	compound	His,p44
R5993	T8875	T8872	conj	p44,p52
R5994	T8876	T8875	punct	-,p44
R5995	T8877	T8875	punct	", ",p44
R5996	T8878	T8879	compound	Flag,p34
R5997	T8879	T8875	conj	p34,p44
R5998	T8880	T8879	punct	-,p34
R5999	T8881	T8879	punct	", ",p34
R6000	T8882	T8879	conj	cdk7,p34
R6001	T8883	T8882	punct	", ",cdk7
R6002	T8884	T8885	compound	cyclin,H
R6003	T8885	T8882	conj	H,cdk7
R6004	T8886	T8885	punct	", ",H
R6005	T8887	T8885	conj	Mat1,H
R6006	T8888	T8887	punct	", ",Mat1
R6007	T8889	T8887	cc	and,Mat1
R6008	T8890	T8887	conj	p8,Mat1
R6009	T8891	T8855	punct	),mixed
R6010	T8892	T8855	auxpass	were,mixed
R6011	T8893	T8855	prep	with,mixed
R6012	T8894	T8895	amod	recombinant,XPG
R6013	T8895	T8893	pobj	XPG,with
R6014	T8896	T8895	punct	", ",XPG
R6015	T8897	T8898	compound	XPF,ERCC1
R6016	T8898	T8895	appos	ERCC1,XPG
R6017	T8899	T8898	punct	/,ERCC1
R6018	T8900	T8895	punct	", ",XPG
R6019	T8901	T8902	compound	XPC,hHR23B
R6020	T8902	T8895	appos	hHR23B,XPG
R6021	T8903	T8902	punct	/,hHR23B
R6022	T8904	T8895	punct	", ",XPG
R6023	T8905	T8895	appos	RPA,XPG
R6024	T8906	T8895	punct	", ",XPG
R6025	T8907	T8895	cc	and,XPG
R6026	T8908	T8909	det	a,substrate
R6027	T8909	T8895	conj	substrate,XPG
R6028	T8910	T8909	amod	radiolabelled,substrate
R6029	T8911	T8909	amod	synthetic,substrate
R6030	T8912	T8909	compound	NER,substrate
R6031	T8913	T8855	punct	.,mixed
R6032	T8915	T8916	det	The,products
R6033	T8916	T8918	nsubjpass	products,visualised
R6034	T8917	T8916	compound	excision,products
R6035	T8919	T8920	punct	(,nucleotides
R6036	T8920	T8916	parataxis	nucleotides,products
R6037	T8921	T8922	quantmod	26,34
R6038	T8922	T8920	nummod	34,nucleotides
R6039	T8923	T8922	punct	–,34
R6040	T8924	T8920	prep	in,nucleotides
R6041	T8925	T8924	pobj	length,in
R6042	T8926	T8920	punct	),nucleotides
R6043	T8927	T8918	auxpass	were,visualised
R6044	T8928	T8918	prep	at,visualised
R6045	T8929	T8930	compound	nucleotide,resolution
R6046	T8930	T8928	pobj	resolution,at
R6047	T8931	T8918	prep	on,visualised
R6048	T8932	T8933	det	a,gel
R6049	T8933	T8931	pobj	gel,on
R6050	T8934	T8933	amod	denaturing,gel
R6051	T8935	T8933	compound	polyacrylamide,gel
R6052	T8936	T8937	mark	as,indicated
R6053	T8937	T8918	advcl	indicated,visualised
R6054	T8938	T8918	punct	.,visualised
R6055	T8941	T8942	det	the,activity
R6056	T8942	T8940	dobj	activity,Note
R6057	T8943	T8942	amod	weak,activity
R6058	T8944	T8942	acl	corresponding,activity
R6059	T8945	T8944	prep	to,corresponding
R6060	T8946	T8947	det	each,complex
R6061	T8947	T8945	pobj	complex,to
R6062	T8948	T8947	amod	single,complex
R6063	T8949	T8948	cc	and,single
R6064	T8950	T8948	conj	combined,single
R6065	T8951	T8947	compound	TFIIH,complex
R6066	T8952	T8953	punct	(,3
R6067	T8953	T8947	parataxis	3,complex
R6068	T8954	T8953	nmod	lanes,3
R6069	T8955	T8956	punct	–,8
R6070	T8956	T8953	prep	8,3
R6071	T8957	T8953	punct	),3
R6072	T8958	T8944	advcl	relative,corresponding
R6073	T8959	T8958	prep	to,relative
R6074	T8960	T8961	det	the,wt
R6075	T8961	T8959	pobj	wt,to
R6076	T8962	T8963	punct	(,lane
R6077	T8963	T8961	parataxis	lane,wt
R6078	T8964	T8963	nummod	1,lane
R6079	T8965	T8963	punct	),lane
R6080	T8966	T8961	cc	and,wt
R6081	T8967	T8968	amod	negative,controls
R6082	T8968	T8961	conj	controls,wt
R6083	T8969	T8970	punct	(,lane
R6084	T8970	T8968	parataxis	lane,controls
R6085	T8971	T8970	nummod	2,lane
R6086	T8972	T8970	punct	),lane
R6087	T8973	T8940	punct	.,Note
R6088	T8975	T8976	punct	(,E
R6089	T8976	T8977	meta	E,reduction
R6090	T8978	T8976	punct	),E
R6091	T8979	T8977	nmod	Xpd,reduction
R6092	T8980	T8981	npadvmod	dose,dependent
R6093	T8981	T8977	amod	dependent,reduction
R6094	T8982	T8981	punct	-,dependent
R6095	T8983	T8977	prep	of,reduction
R6096	T8984	T8983	pobj	TFIIH,of
R6097	T8985	T8977	prep	in,reduction
R6098	T8986	T8987	amod	homozygous,TTD
R6099	T8987	T8990	nmod	TTD,cells
R6100	T8988	T8987	nmod	XpdTTD,TTD
R6101	T8989	T8987	punct	/,TTD
R6102	T8990	T8985	pobj	cells,in
R6103	T8991	T8987	punct	", ",TTD
R6104	T8992	T8993	amod	hemizygous,KO
R6105	T8993	T8987	conj	KO,TTD
R6106	T8994	T8993	compound	XpdTTD,KO
R6107	T8995	T8993	punct	/,KO
R6108	T8996	T8993	punct	", ",KO
R6109	T8997	T8993	cc	and,KO
R6110	T8998	T8999	nmod	compound,†XPCS
R6111	T8999	T8993	conj	†XPCS,KO
R6112	T9000	T8999	amod	heterozygous,†XPCS
R6113	T9001	T8999	compound	XpdTTD,†XPCS
R6114	T9002	T8999	punct	/,†XPCS
R6115	T9003	T8999	cc	and,†XPCS
R6116	T9004	T9005	compound	XpdTTD,†XP
R6117	T9005	T8999	conj	†XP,†XPCS
R6118	T9006	T9005	punct	/,†XP
R6119	T9007	T8977	prep	by,reduction
R6120	T9008	T9009	amod	comparative,immunofluorescence
R6121	T9009	T9007	pobj	immunofluorescence,by
R6122	T9010	T9009	prep	of,immunofluorescence
R6123	T9011	T9012	det	the,subunit
R6124	T9012	T9010	pobj	subunit,of
R6125	T9013	T9012	compound	p62,subunit
R6126	T9014	T9012	prep	of,subunit
R6127	T9015	T9014	pobj	TFIIH,of
R6128	T9016	T8977	punct	.,reduction
R6129	T9018	T9019	amod	Roman,numerals
R6130	T9019	T9020	nsubj	numerals,represent
R6131	T9021	T9022	amod	different,slides
R6132	T9022	T9020	dobj	slides,represent
R6133	T9023	T9022	amod	microscopic,slides
R6134	T9024	T9020	cc	and,represent
R6135	T9025	T9026	amod	Arabic,numerals
R6136	T9026	T9027	nsubj	numerals,lines
R6137	T9027	T9020	conj	lines,represent
R6138	T9028	T9027	amod	different,lines
R6139	T9029	T9027	compound	cell,lines
R6140	T9030	T9027	acl	labelled,lines
R6141	T9031	T9032	mark	as,follows
R6142	T9032	T9030	advcl	follows,labelled
R6143	T9033	T9027	punct	: ,lines
R6144	T9034	T9035	punct	(,I
R6145	T9035	T9036	meta	I,cells
R6146	T9036	T9027	dep	cells,lines
R6147	T9037	T9035	punct	),I
R6148	T9038	T9036	compound	wt,cells
R6149	T9039	T9040	punct	(,1
R6150	T9040	T9036	parataxis	1,cells
R6151	T9041	T9040	punct	),1
R6152	T9042	T9036	acl	labelled,cells
R6153	T9043	T9042	prep	with,labelled
R6154	T9044	T9045	nummod	2,μm
R6155	T9045	T9047	compound	μm,beads
R6156	T9046	T9045	punct	-,μm
R6157	T9047	T9043	pobj	beads,with
R6158	T9048	T9036	punct	", ",cells
R6159	T9049	T9050	compound	XpdTTD,TTD
R6160	T9050	T9052	compound	TTD,cells
R6161	T9051	T9050	punct	/,TTD
R6162	T9052	T9036	conj	cells,cells
R6163	T9053	T9054	punct	(,2
R6164	T9054	T9052	parataxis	2,cells
R6165	T9055	T9054	punct	),2
R6166	T9056	T9052	prep	with,cells
R6167	T9057	T9058	nummod	0.79,μm
R6168	T9058	T9060	compound	μm,beads
R6169	T9059	T9058	punct	-,μm
R6170	T9060	T9056	pobj	beads,with
R6171	T9061	T9052	punct	", ",cells
R6172	T9062	T9052	cc	and,cells
R6173	T9063	T9064	compound	XpdTTD,KO
R6174	T9064	T9066	compound	KO,cells
R6175	T9065	T9064	punct	/,KO
R6176	T9066	T9052	conj	cells,cells
R6177	T9067	T9068	punct	(,3
R6178	T9068	T9066	parataxis	3,cells
R6179	T9069	T9068	punct	),3
R6180	T9070	T9066	prep	with,cells
R6181	T9071	T9072	det	no,beads
R6182	T9072	T9070	pobj	beads,with
R6183	T9073	T9036	punct	;,cells
R6184	T9074	T9075	punct	(,II
R6185	T9075	T9076	meta	II,cells
R6186	T9076	T9036	conj	cells,cells
R6187	T9077	T9075	punct	),II
R6188	T9078	T9076	compound	wt,cells
R6189	T9079	T9080	punct	(,1
R6190	T9080	T9076	parataxis	1,cells
R6191	T9081	T9080	punct	),1
R6192	T9082	T9076	acl	labelled,cells
R6193	T9083	T9082	prep	with,labelled
R6194	T9084	T9085	nummod	0.79,μm
R6195	T9085	T9087	compound	μm,beads
R6196	T9086	T9085	punct	-,μm
R6197	T9087	T9083	pobj	beads,with
R6198	T9088	T9076	cc	and,cells
R6199	T9089	T9090	compound	XpdTTD,†XPCS
R6200	T9090	T9092	compound	†XPCS,cells
R6201	T9091	T9090	punct	/,†XPCS
R6202	T9092	T9076	conj	cells,cells
R6203	T9093	T9094	punct	(,4
R6204	T9094	T9092	parataxis	4,cells
R6205	T9095	T9094	punct	),4
R6206	T9096	T9092	prep	with,cells
R6207	T9097	T9098	det	no,beads
R6208	T9098	T9096	pobj	beads,with
R6209	T9099	T9076	punct	;,cells
R6210	T9100	T9076	cc	and,cells
R6211	T9101	T9102	punct	(,III
R6212	T9102	T9103	meta	III,cells
R6213	T9103	T9076	conj	cells,cells
R6214	T9104	T9102	punct	),III
R6215	T9105	T9103	compound	wt,cells
R6216	T9106	T9107	punct	(,1
R6217	T9107	T9103	parataxis	1,cells
R6218	T9108	T9107	punct	),1
R6219	T9109	T9103	acl	labelled,cells
R6220	T9110	T9109	prep	with,labelled
R6221	T9111	T9112	nummod	0.79,μm
R6222	T9112	T9114	compound	μm,beads
R6223	T9113	T9112	punct	-,μm
R6224	T9114	T9110	pobj	beads,with
R6225	T9115	T9103	cc	and,cells
R6226	T9116	T9117	compound	XpdTTD,†XP
R6227	T9117	T9119	compound	†XP,cells
R6228	T9118	T9117	punct	/,†XP
R6229	T9119	T9103	conj	cells,cells
R6230	T9120	T9121	punct	(,5
R6231	T9121	T9119	parataxis	5,cells
R6232	T9122	T9121	punct	),5
R6233	T9123	T9119	prep	with,cells
R6234	T9124	T9125	det	no,beads
R6235	T9125	T9123	pobj	beads,with
R6236	T9126	T9027	punct	.,lines
R6237	T9128	T9129	punct	(,F
R6238	T9129	T9130	meta	F,Quantification
R6239	T9131	T9129	punct	),F
R6240	T9132	T9130	prep	of,Quantification
R6241	T9133	T9134	amod	immunofluorescent,signal
R6242	T9134	T9132	pobj	signal,of
R6243	T9135	T9134	prep	from,signal
R6244	T9136	T9137	advmod	at,50
R6245	T9137	T9139	nummod	50,nuclei
R6246	T9138	T9137	advmod	least,50
R6247	T9139	T9135	pobj	nuclei,from
R6248	T9140	T9139	prep	per,nuclei
R6249	T9141	T9142	compound	cell,line
R6250	T9142	T9140	pobj	line,per
R6251	T9143	T9139	cc	and,nuclei
R6252	T9144	T9145	quantmod	2,6
R6253	T9145	T9147	nummod	6,experiments
R6254	T9146	T9145	punct	–,6
R6255	T9147	T9139	conj	experiments,nuclei
R6256	T9148	T9147	prep	per,experiments
R6257	T9149	T9148	pobj	genotype,per
R6258	T9150	T9130	punct	.,Quantification
R6259	T9152	T9153	nsubjpass	Bars,depicted
R6260	T9154	T9152	acl	representing,Bars
R6261	T9155	T9154	dobj	cells,representing
R6262	T9156	T9155	acl	analysed,cells
R6263	T9157	T9156	prep	on,analysed
R6264	T9158	T9159	det	the,slide
R6265	T9159	T9157	pobj	slide,on
R6266	T9160	T9159	amod	same,slide
R6267	T9161	T9159	amod	microscopic,slide
R6268	T9162	T9153	auxpass	are,depicted
R6269	T9163	T9153	npadvmod	side,depicted
R6270	T9164	T9163	prep	by,side
R6271	T9165	T9164	pobj	side,by
R6272	T9166	T9153	punct	", ",depicted
R6273	T9167	T9153	prep	with,depicted
R6274	T9168	T9167	pobj	wt,with
R6275	T9169	T9168	acl	set,wt
R6276	T9170	T9169	prep	at,set
R6277	T9171	T9172	nummod	100,%
R6278	T9172	T9170	pobj	%,at
R6279	T9173	T9153	punct	.,depicted
R6280	T9175	T9176	det	The,value
R6281	T9176	T9179	nsubj	value,indicates
R6282	T9177	T9176	compound	p,value
R6283	T9178	T9176	punct	-,value
R6284	T9180	T9181	amod	minimum,difference
R6285	T9181	T9179	dobj	difference,indicates
R6286	T9182	T9181	amod	significant,difference
R6287	T9183	T9181	prep	between,difference
R6288	T9184	T9183	pobj	wt,between
R6289	T9185	T9184	cc	and,wt
R6290	T9186	T9187	det	the,lines
R6291	T9187	T9184	conj	lines,wt
R6292	T9188	T9187	amod	indicated,lines
R6293	T9189	T9187	compound	cell,lines
R6294	T9190	T9187	acl	analysed,lines
R6295	T9191	T9190	prep	on,analysed
R6296	T9192	T9193	det	the,slide
R6297	T9193	T9191	pobj	slide,on
R6298	T9194	T9193	amod	same,slide
R6299	T9195	T9193	amod	microscopic,slide
R6300	T9196	T9181	prep	within,difference
R6301	T9197	T9198	nummod	one,experiment
R6302	T9198	T9196	pobj	experiment,within
R6303	T9199	T9179	punct	.,indicates
R6304	T9212	T9211	prep	of,Frequency
R6305	T9213	T9214	compound	Xpd†XP,†XP
R6306	T9214	T9212	pobj	†XP,of
R6307	T9215	T9214	punct	/,†XP
R6308	T9216	T9214	punct	", ",†XP
R6309	T9217	T9218	compound	Xpd†XPCS,†XPCS
R6310	T9218	T9214	conj	†XPCS,†XP
R6311	T9219	T9218	punct	/,†XPCS
R6312	T9220	T9218	punct	", ",†XPCS
R6313	T9221	T9218	cc	and,†XPCS
R6314	T9222	T9223	nmod	Compound,†XPCS
R6315	T9223	T9218	conj	†XPCS,†XPCS
R6316	T9224	T9223	amod	Heterozygous,†XPCS
R6317	T9225	T9223	compound	Xpd†XP,†XPCS
R6318	T9226	T9223	punct	/,†XPCS
R6319	T9227	T9214	appos	Embryos,†XP
R6320	T9228	T9227	cc	and,Embryos
R6321	T9229	T9227	conj	Offspring,Embryos
R6322	T9241	T9242	amod	Pleiotropic,Effects
R6323	T9243	T9242	nmod	Xpd,Effects
R6324	T9244	T9242	amod	Biallelic,Effects
R6325	T9245	T9242	prep	in,Effects
R6326	T9246	T9245	pobj	Mice,in
R6327	T9247	T9246	cc	and,Mice
R6328	T9248	T9246	conj	Cells,Mice
R910	T1401	T1400	prep	of,Generation
R911	T1402	T1403	compound	Xpd,Heterozygotes
R912	T1403	T1401	pobj	Heterozygotes,of
R913	T1404	T1403	compound	Compound,Heterozygotes
R914	T1406	T1407	nsubj	We,generated
R915	T1408	T1409	det	an,allele
R916	T1409	T1407	dobj	allele,generated
R917	T1410	T1409	nmod	Xpd,allele
R918	T1411	T1409	amod	knock,allele
R919	T1412	T1411	punct	-,knock
R920	T1413	T1411	prt	in,knock
R921	T1414	T1409	prep	with,allele
R922	T1415	T1416	det	a,mutation
R923	T1416	T1414	pobj	mutation,with
R924	T1417	T1416	compound	point,mutation
R925	T1418	T1416	acl	encoding,mutation
R926	T1419	T1420	det	a,change
R927	T1420	T1418	dobj	change,encoding
R928	T1421	T1420	amod	single,change
R929	T1422	T1423	compound	amino,acid
R930	T1423	T1420	compound	acid,change
R931	T1424	T1425	punct	(,XPDG602D
R932	T1425	T1420	parataxis	XPDG602D,change
R933	T1426	T1425	punct	),XPDG602D
R934	T1427	T1420	acl	found,change
R935	T1428	T1427	prep	in,found
R936	T1429	T1430	det	the,patient
R937	T1430	T1428	pobj	patient,in
R938	T1431	T1430	compound	XPCS,patient
R939	T1432	T1430	appos	XPCS2,patient
R940	T1433	T1434	punct	(,1A
R941	T1434	T1407	parataxis	1A,generated
R942	T1435	T1434	compound	Figure,1A
R943	T1436	T1437	punct	–,1C
R944	T1437	T1434	prep	1C,1A
R945	T1438	T1434	punct	),1A
R946	T1439	T1407	punct	.,generated
R947	T1441	T1442	compound	mRNA,expression
R948	T1442	T1443	nsubjpass	expression,detected
R949	T1444	T1442	prep	from,expression
R950	T1445	T1446	det	the,allele
R951	T1446	T1444	pobj	allele,from
R952	T1447	T1446	amod	targeted,allele
R953	T1448	T1443	aux	could,detected
R954	T1449	T1443	auxpass	be,detected
R955	T1450	T1443	prep	in,detected
R956	T1451	T1452	amod	embryonic,cells
R957	T1452	T1450	pobj	cells,in
R958	T1453	T1452	compound	stem,cells
R959	T1454	T1443	prep	by,detected
R960	T1455	T1456	compound	RT,PCR
R961	T1456	T1454	pobj	PCR,by
R962	T1457	T1456	punct	-,PCR
R963	T1458	T1459	punct	(,1D
R964	T1459	T1443	parataxis	1D,detected
R965	T1460	T1459	compound	Figure,1D
R966	T1461	T1459	punct	),1D
R967	T1462	T1443	punct	", ",detected
R968	T1463	T1464	mark	although,reduced
R969	T1464	T1443	advcl	reduced,detected
R970	T1465	T1464	nsubjpass	expression,reduced
R971	T1466	T1464	auxpass	was,reduced
R972	T1467	T1468	advmod	approximately,5
R973	T1468	T1464	npadvmod	5,reduced
R974	T1469	T1468	punct	-,5
R975	T1470	T1468	advmod	fold,5
R976	T1471	T1464	advcl	relative,reduced
R977	T1472	T1471	prep	to,relative
R978	T1473	T1474	compound	wt,levels
R979	T1474	T1472	pobj	levels,to
R980	T1475	T1474	compound	mRNA,levels
R981	T1476	T1474	compound	transcript,levels
R982	T1477	T1478	mark	as,determined
R983	T1478	T1464	advcl	determined,reduced
R984	T1479	T1478	prep	by,determined
R985	T1480	T1479	pobj	Northern,by
R986	T1481	T1480	amod	blotting,Northern
R987	T1482	T1480	prep	of,Northern
R988	T1483	T1482	pobj	RNA,of
R989	T1484	T1483	prep	from,RNA
R990	T1485	T1486	det	the,testis
R991	T1486	T1484	pobj	testis,from
R992	T1487	T1486	prep	of,testis
R993	T1488	T1489	amod	heterozygous,animals
R994	T1489	T1487	pobj	animals,of
R995	T1490	T1491	punct	(,1E
R996	T1491	T1443	parataxis	1E,detected
R997	T1492	T1491	compound	Figure,1E
R998	T1493	T1491	punct	),1E
R999	T1494	T1443	punct	.,detected

PMC:1584416 / 5817-24237 JSONTXT 5 Projects

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PMC:1584416 / 5817-24237 JSON TXT 5 Projects