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Id Subject Object Predicate Lexical cue
T4597 9-19 NN denotes Generation
T4598 20-22 IN denotes of
T4599 23-26 DT denotes the
T4601 27-33 NN denotes ApcCKO
T4602 34-37 CC denotes and
T4603 38-45 NN denotes ApcΔ580
T4600 46-50 NNS denotes Mice
T4604 50-217 sentence denotes To investigate the role of Apc in development of skin and its appendages, we used the Cre/loxP technology to introduce a conditional mutation of the Apc gene in mice.
T4605 51-53 TO denotes To
T4606 54-65 VB denotes investigate
T4608 66-69 DT denotes the
T4609 70-74 NN denotes role
T4610 75-77 IN denotes of
T4611 78-81 NN denotes Apc
T4612 82-84 IN denotes in
T4613 85-96 NN denotes development
T4614 97-99 IN denotes of
T4615 100-104 NN denotes skin
T4616 105-108 CC denotes and
T4617 109-112 PRP$ denotes its
T4618 113-123 NNS denotes appendages
T4619 123-125 , denotes ,
T4620 125-127 PRP denotes we
T4607 128-132 VBD denotes used
T4621 133-136 DT denotes the
T4623 137-140 NN denotes Cre
T4625 140-141 HYPH denotes /
T4624 141-145 NN denotes loxP
T4622 146-156 NN denotes technology
T4626 157-159 TO denotes to
T4627 160-169 VB denotes introduce
T4628 170-171 DT denotes a
T4630 172-183 JJ denotes conditional
T4629 184-192 NN denotes mutation
T4631 193-195 IN denotes of
T4632 196-199 DT denotes the
T4634 200-203 NN denotes Apc
T4633 204-208 NN denotes gene
T4635 209-211 IN denotes in
T4636 212-216 NNS denotes mice
T4637 216-217 . denotes .
T4638 217-517 sentence denotes We constructed embryonic stem (ES) cells and mice carrying an Apc allele harboring both a pair of loxP sites flanking Apc exon 14 and a pair of FLP recognition target (FRT) sites flanking PGK-neomycin selection cassette by recombineering [12,13] (Figure 1A, ApcCKON allele, N for neomycin cassette).
T4639 218-220 PRP denotes We
T4640 221-232 VBD denotes constructed
T4641 233-242 JJ denotes embryonic
T4642 243-247 NN denotes stem
T4644 248-249 -LRB- denotes (
T4645 249-251 NN denotes ES
T4646 251-252 -RRB- denotes )
T4643 253-258 NNS denotes cells
T4647 259-262 CC denotes and
T4648 263-267 NNS denotes mice
T4649 268-276 VBG denotes carrying
T4650 277-279 DT denotes an
T4652 280-283 NN denotes Apc
T4651 284-290 NN denotes allele
T4653 291-300 VBG denotes harboring
T4654 301-305 CC denotes both
T4656 306-307 DT denotes a
T4655 308-312 NN denotes pair
T4657 313-315 IN denotes of
T4658 316-320 NN denotes loxP
T4659 321-326 NNS denotes sites
T4660 327-335 VBG denotes flanking
T4661 336-339 NN denotes Apc
T4662 340-344 NN denotes exon
T4663 345-347 CD denotes 14
T4664 348-351 CC denotes and
T4665 352-353 DT denotes a
T4666 354-358 NN denotes pair
T4667 359-361 IN denotes of
T4668 362-365 NN denotes FLP
T4670 366-377 NN denotes recognition
T4669 378-384 NN denotes target
T4672 385-386 -LRB- denotes (
T4673 386-389 NN denotes FRT
T4674 389-390 -RRB- denotes )
T4671 391-396 NNS denotes sites
T4675 397-405 VBG denotes flanking
T4676 406-409 NN denotes PGK
T4678 409-410 HYPH denotes -
T4677 410-418 NN denotes neomycin
T4680 419-428 NN denotes selection
T4679 429-437 NN denotes cassette
T4681 438-440 IN denotes by
T4682 441-455 VBG denotes recombineering
T4683 456-457 -LRB- denotes [
T4685 457-459 CD denotes 12
T4686 459-460 , denotes ,
T4684 460-462 CD denotes 13
T4687 462-463 -RRB- denotes ]
T4688 464-465 -LRB- denotes (
T4690 465-471 NN denotes Figure
T4691 472-474 NN denotes 1A
T4692 474-476 , denotes ,
T4693 476-483 NN denotes ApcCKON
T4694 484-490 NN denotes allele
T4695 490-492 , denotes ,
T4689 492-493 NN denotes N
T4696 494-497 IN denotes for
T4697 498-506 NN denotes neomycin
T4698 507-515 NN denotes cassette
T4699 515-516 -RRB- denotes )
T4700 516-517 . denotes .
T4701 517-638 sentence denotes A PGK-neomycin cassette was inserted in the same transcriptional orientation as Apc in intron 14 of the endogenous gene.
T4702 518-519 DT denotes A
T4704 520-523 NN denotes PGK
T4706 523-524 HYPH denotes -
T4705 524-532 NN denotes neomycin
T4703 533-541 NN denotes cassette
T4708 542-545 VBD denotes was
T4707 546-554 VBN denotes inserted
T4709 555-557 IN denotes in
T4710 558-561 DT denotes the
T4712 562-566 JJ denotes same
T4713 567-582 JJ denotes transcriptional
T4711 583-594 NN denotes orientation
T4714 595-597 IN denotes as
T4715 598-601 NN denotes Apc
T4716 602-604 IN denotes in
T4717 605-611 NN denotes intron
T4718 612-614 CD denotes 14
T4719 615-617 IN denotes of
T4720 618-621 DT denotes the
T4722 622-632 JJ denotes endogenous
T4721 633-637 NN denotes gene
T4723 637-638 . denotes .
T4724 638-716 sentence denotes The loxP and FRT sites were used to aid unidirectional recombination [12,13].
T4725 639-642 DT denotes The
T4727 643-647 NN denotes loxP
T4728 648-651 CC denotes and
T4729 652-655 NN denotes FRT
T4726 656-661 NNS denotes sites
T4731 662-666 VBD denotes were
T4730 667-671 VBN denotes used
T4732 672-674 TO denotes to
T4733 675-678 VB denotes aid
T4734 679-693 JJ denotes unidirectional
T4735 694-707 NN denotes recombination
T4736 708-709 -LRB- denotes [
T4738 709-711 CD denotes 12
T4739 711-712 , denotes ,
T4737 712-714 CD denotes 13
T4740 714-715 -RRB- denotes ]
T4741 715-716 . denotes .
T4742 716-866 sentence denotes Two mouse lines containing the same modification were generated from two independent ES clones to ensure that these two lines behave in the same way.
T4743 717-720 CD denotes Two
T4745 721-726 NN denotes mouse
T4744 727-732 NNS denotes lines
T4747 733-743 VBG denotes containing
T4748 744-747 DT denotes the
T4750 748-752 JJ denotes same
T4749 753-765 NN denotes modification
T4751 766-770 VBD denotes were
T4746 771-780 VBN denotes generated
T4752 781-785 IN denotes from
T4753 786-789 CD denotes two
T4755 790-801 JJ denotes independent
T4756 802-804 NN denotes ES
T4754 805-811 NNS denotes clones
T4757 812-814 TO denotes to
T4758 815-821 VB denotes ensure
T4759 822-826 IN denotes that
T4761 827-832 DT denotes these
T4763 833-836 CD denotes two
T4762 837-842 NNS denotes lines
T4760 843-849 VBP denotes behave
T4764 850-852 IN denotes in
T4765 853-856 DT denotes the
T4767 857-861 JJ denotes same
T4766 862-865 NN denotes way
T4768 865-866 . denotes .
T4769 866-1118 sentence denotes These ApcCKON/+ mice were crossed with FLPe-deleter to generate ApcCKO/+ mice that were heterozygous for the final Apc conditional (ApcCKO) allele that removed the PGK-neomycin cassette and contains only the loxP sites in the introns flanking exon 14.
T4770 867-872 DT denotes These
T4772 873-880 NN denotes ApcCKON
T4773 880-881 HYPH denotes /
T4774 881-882 SYM denotes +
T4771 883-887 NNS denotes mice
T4776 888-892 VBD denotes were
T4775 893-900 VBN denotes crossed
T4777 901-905 IN denotes with
T4778 906-910 NN denotes FLPe
T4780 910-911 HYPH denotes -
T4779 911-918 NN denotes deleter
T4781 919-921 TO denotes to
T4782 922-930 VB denotes generate
T4783 931-937 NN denotes ApcCKO
T4785 937-938 HYPH denotes /
T4786 938-939 SYM denotes +
T4784 940-944 NNS denotes mice
T4787 945-949 WDT denotes that
T4788 950-954 VBD denotes were
T4789 955-967 JJ denotes heterozygous
T4790 968-971 IN denotes for
T4791 972-975 DT denotes the
T4793 976-981 JJ denotes final
T4794 982-985 NN denotes Apc
T4795 986-997 JJ denotes conditional
T4796 998-999 -LRB- denotes (
T4797 999-1005 NN denotes ApcCKO
T4798 1005-1006 -RRB- denotes )
T4792 1007-1013 NN denotes allele
T4799 1014-1018 WDT denotes that
T4800 1019-1026 VBD denotes removed
T4801 1027-1030 DT denotes the
T4803 1031-1034 NN denotes PGK
T4805 1034-1035 HYPH denotes -
T4804 1035-1043 NN denotes neomycin
T4802 1044-1052 NN denotes cassette
T4806 1053-1056 CC denotes and
T4807 1057-1065 VBZ denotes contains
T4808 1066-1070 RB denotes only
T4810 1071-1074 DT denotes the
T4811 1075-1079 NN denotes loxP
T4809 1080-1085 NNS denotes sites
T4812 1086-1088 IN denotes in
T4813 1089-1092 DT denotes the
T4814 1093-1100 NNS denotes introns
T4815 1101-1109 VBG denotes flanking
T4816 1110-1114 NN denotes exon
T4817 1115-1117 CD denotes 14
T4818 1117-1118 . denotes .
T4819 1118-1299 sentence denotes To assess the effect of deleting exon 14 in mice, both lines of ApcCKO/+ mice were crossed with the Cre-deleter to generate the germline knockout line of Apc, designated ApcΔ580/+.
T4820 1119-1121 TO denotes To
T4821 1122-1128 VB denotes assess
T4823 1129-1132 DT denotes the
T4824 1133-1139 NN denotes effect
T4825 1140-1142 IN denotes of
T4826 1143-1151 VBG denotes deleting
T4827 1152-1156 NN denotes exon
T4828 1157-1159 CD denotes 14
T4829 1160-1162 IN denotes in
T4830 1163-1167 NNS denotes mice
T4831 1167-1169 , denotes ,
T4832 1169-1173 DT denotes both
T4833 1174-1179 NNS denotes lines
T4834 1180-1182 IN denotes of
T4835 1183-1189 NN denotes ApcCKO
T4837 1189-1190 HYPH denotes /
T4838 1190-1191 SYM denotes +
T4836 1192-1196 NNS denotes mice
T4839 1197-1201 VBD denotes were
T4822 1202-1209 VBN denotes crossed
T4840 1210-1214 IN denotes with
T4841 1215-1218 DT denotes the
T4843 1219-1222 NN denotes Cre
T4844 1222-1223 HYPH denotes -
T4842 1223-1230 NN denotes deleter
T4845 1231-1233 TO denotes to
T4846 1234-1242 VB denotes generate
T4847 1243-1246 DT denotes the
T4849 1247-1255 NN denotes germline
T4850 1256-1264 NN denotes knockout
T4848 1265-1269 NN denotes line
T4851 1270-1272 IN denotes of
T4852 1273-1276 NNP denotes Apc
T4853 1276-1278 , denotes ,
T4854 1278-1288 VBN denotes designated
T4855 1289-1296 NN denotes ApcΔ580
T4856 1296-1297 HYPH denotes /
T4857 1297-1298 SYM denotes +
T4858 1298-1299 . denotes .
T4859 1299-1354 sentence denotes The mutant allele (ApcΔ580) lacks exon 14 (Figure 1A).
T4860 1300-1303 DT denotes The
T4862 1304-1310 JJ denotes mutant
T4861 1311-1317 NN denotes allele
T4864 1318-1319 -LRB- denotes (
T4865 1319-1326 NN denotes ApcΔ580
T4866 1326-1327 -RRB- denotes )
T4863 1328-1333 VBZ denotes lacks
T4867 1334-1338 NN denotes exon
T4868 1339-1341 CD denotes 14
T4869 1342-1343 -LRB- denotes (
T4871 1343-1349 NN denotes Figure
T4870 1350-1352 NN denotes 1A
T4872 1352-1353 -RRB- denotes )
T4873 1353-1354 . denotes .
T4874 1354-1637 sentence denotes The transcript from loss of exon 14 results in a shift in the normal reading frame, resulting in a premature chain termination codon which, if utilized, would result in a truncated polypeptide that is 605 aa in length, of which the first 580 aa correspond to the normal Apc protein.
T4875 1355-1358 DT denotes The
T4876 1359-1369 NN denotes transcript
T4878 1370-1374 IN denotes from
T4879 1375-1379 NN denotes loss
T4880 1380-1382 IN denotes of
T4881 1383-1387 NN denotes exon
T4882 1388-1390 CD denotes 14
T4877 1391-1398 VBZ denotes results
T4883 1399-1401 IN denotes in
T4884 1402-1403 DT denotes a
T4885 1404-1409 NN denotes shift
T4886 1410-1412 IN denotes in
T4887 1413-1416 DT denotes the
T4889 1417-1423 JJ denotes normal
T4890 1424-1431 NN denotes reading
T4888 1432-1437 NN denotes frame
T4891 1437-1439 , denotes ,
T4892 1439-1448 VBG denotes resulting
T4893 1449-1451 IN denotes in
T4894 1452-1453 DT denotes a
T4896 1454-1463 JJ denotes premature
T4897 1464-1469 NN denotes chain
T4898 1470-1481 NN denotes termination
T4895 1482-1487 NN denotes codon
T4899 1488-1493 WDT denotes which
T4901 1493-1495 , denotes ,
T4902 1495-1497 IN denotes if
T4903 1498-1506 VBN denotes utilized
T4904 1506-1508 , denotes ,
T4905 1508-1513 MD denotes would
T4900 1514-1520 VB denotes result
T4906 1521-1523 IN denotes in
T4907 1524-1525 DT denotes a
T4909 1526-1535 VBN denotes truncated
T4908 1536-1547 NN denotes polypeptide
T4910 1548-1552 WDT denotes that
T4911 1553-1555 VBZ denotes is
T4912 1556-1559 CD denotes 605
T4913 1560-1562 NNS denotes aa
T4914 1563-1565 IN denotes in
T4915 1566-1572 NN denotes length
T4916 1572-1574 , denotes ,
T4917 1574-1576 IN denotes of
T4919 1577-1582 WDT denotes which
T4920 1583-1586 DT denotes the
T4922 1587-1592 JJ denotes first
T4923 1593-1596 CD denotes 580
T4921 1597-1599 NNS denotes aa
T4918 1600-1610 VBP denotes correspond
T4924 1611-1613 IN denotes to
T4925 1614-1617 DT denotes the
T4927 1618-1624 JJ denotes normal
T4928 1625-1628 NN denotes Apc
T4926 1629-1636 NN denotes protein
T4929 1636-1637 . denotes .
T4930 1637-3361 sentence denotes Figure 1 Generation of the Conditional Apc Allele (A) Schematic diagram of exons 14 and 15 of the mouse Apc gene, the targeting vector, and the resulting conditional allele with 2 LoxP sites sandwiching the exon 14. The PGK-neomycin cassette was inserted within intron 14 by recombineering technique. This cassette is sandwiched by 2 FRT sites that could be removed by crossing to FLPe-expressing mice. Positions of PCR primers used for genotyping PCR (F2, R2, R4) and RT-PCR (F546 and R721) are indicated. Positions of probe used for Southern blot analysis with NdeI sites are also shown. Upon Cre-mediated recombination, exon 14 is removed and leads to truncated Apc protein, of which the first 580 aa correspond to the normal. (B) Southern blot analysis of NdeI-digested genomic tail DNA isolated from F1 mice of various Apc mouse lines (ApcCKON, ApcΔ580), hybridized to a 600-bp probe. Tail genomic DNA from ApcCKON F1 mice derived from a modified ES clone showed a 12-kb band for the ApcCKON allele and a 10-kb band for the wild-type allele, whereas genomic DNA from the ApcΔ580 mouse was heterozygous for the ApcΔ580 allele (9.2-kb band). (C) Kaplan-Meier survival plot of ApcCKO/+ mice (thin solid line, n = 39), ApcCKO/CKO mice (thin dotted line, n = 57), ApcΔ580/+ mice (solid line, n = 51), and wild-type littermates (broken line, n = 21). Heterozygosity of the ApcΔ580 allele led to a significantly shortened survival (p < 0.0001), whereas those of heterozygous and homozygous ApcCKO mice had no significant difference to that of wild-type littermates. Southern blot analysis of tail DNA from F1 offspring of both ApcCKON and ApcΔ580 lines confirmed the germline transmission of modified Apc allele (Figure 1B).
T4931 3203-3211 NNP denotes Southern
T4932 3212-3216 NN denotes blot
T4933 3217-3225 NN denotes analysis
T4935 3226-3228 IN denotes of
T4936 3229-3233 NN denotes tail
T4937 3234-3237 NN denotes DNA
T4938 3238-3242 IN denotes from
T4939 3243-3245 NN denotes F1
T4940 3246-3255 NN denotes offspring
T4941 3256-3258 IN denotes of
T4942 3259-3263 CC denotes both
T4943 3264-3271 NN denotes ApcCKON
T4945 3272-3275 CC denotes and
T4946 3276-3283 NN denotes ApcΔ580
T4944 3284-3289 NNS denotes lines
T4934 3290-3299 VBD denotes confirmed
T4947 3300-3303 DT denotes the
T4949 3304-3312 NN denotes germline
T4948 3313-3325 NN denotes transmission
T4950 3326-3328 IN denotes of
T4951 3329-3337 VBN denotes modified
T4953 3338-3341 NN denotes Apc
T4952 3342-3348 NN denotes allele
T4954 3349-3350 -LRB- denotes (
T4956 3350-3356 NN denotes Figure
T4955 3357-3359 NN denotes 1B
T4957 3359-3360 -RRB- denotes )
T4958 3360-3361 . denotes .
T4959 3361-3475 sentence denotes Mice that are heterozygous for ApcΔ580 mutation are viable but have a significantly reduced lifespan (Figure 1C).
T4960 3362-3366 NNS denotes Mice
T4962 3367-3371 WDT denotes that
T4963 3372-3375 VBP denotes are
T4964 3376-3388 JJ denotes heterozygous
T4965 3389-3392 IN denotes for
T4966 3393-3400 NN denotes ApcΔ580
T4967 3401-3409 NN denotes mutation
T4961 3410-3413 VBP denotes are
T4968 3414-3420 JJ denotes viable
T4969 3421-3424 CC denotes but
T4970 3425-3429 VBP denotes have
T4971 3430-3431 DT denotes a
T4973 3432-3445 RB denotes significantly
T4974 3446-3453 VBN denotes reduced
T4972 3454-3462 NN denotes lifespan
T4975 3463-3464 -LRB- denotes (
T4977 3464-3470 NN denotes Figure
T4976 3471-3473 NN denotes 1C
T4978 3473-3474 -RRB- denotes )
T4979 3474-3475 . denotes .
T4980 3475-3600 sentence denotes These results suggested that deletion of exon 14 indeed results in either loss or abnormal function of the Apc gene product.
T4981 3476-3481 DT denotes These
T4982 3482-3489 NNS denotes results
T4983 3490-3499 VBD denotes suggested
T4984 3500-3504 IN denotes that
T4986 3505-3513 NN denotes deletion
T4987 3514-3516 IN denotes of
T4988 3517-3521 NN denotes exon
T4989 3522-3524 CD denotes 14
T4990 3525-3531 RB denotes indeed
T4985 3532-3539 VBZ denotes results
T4991 3540-3542 IN denotes in
T4992 3543-3549 CC denotes either
T4993 3550-3554 NN denotes loss
T4994 3555-3557 CC denotes or
T4995 3558-3566 JJ denotes abnormal
T4996 3567-3575 NN denotes function
T4997 3576-3578 IN denotes of
T4998 3579-3582 DT denotes the
T5000 3583-3586 NN denotes Apc
T5001 3587-3591 NN denotes gene
T4999 3592-3599 NN denotes product
T5002 3599-3600 . denotes .
T5003 3600-3718 sentence denotes ApcΔ580/+ mice have median survival of 5 mo of age (Figure 1C), with progressive signs of rectal bleeding and anemia.
T5004 3601-3608 NN denotes ApcΔ580
T5006 3608-3609 HYPH denotes /
T5007 3609-3610 SYM denotes +
T5005 3611-3615 NNS denotes mice
T5008 3616-3620 VBP denotes have
T5009 3621-3627 JJ denotes median
T5010 3628-3636 NN denotes survival
T5011 3637-3639 IN denotes of
T5012 3640-3641 CD denotes 5
T5013 3642-3644 NNS denotes mo
T5014 3645-3647 IN denotes of
T5015 3648-3651 NN denotes age
T5016 3652-3653 -LRB- denotes (
T5018 3653-3659 NN denotes Figure
T5017 3660-3662 NN denotes 1C
T5019 3662-3663 -RRB- denotes )
T5020 3663-3665 , denotes ,
T5021 3665-3669 IN denotes with
T5022 3670-3681 JJ denotes progressive
T5023 3682-3687 NNS denotes signs
T5024 3688-3690 IN denotes of
T5025 3691-3697 JJ denotes rectal
T5026 3698-3706 NN denotes bleeding
T5027 3707-3710 CC denotes and
T5028 3711-3717 NN denotes anemia
T5029 3717-3718 . denotes .
T5030 3718-3933 sentence denotes Similar to the results reported with an independently generated ApcΔ580/+ conditional mouse strain [14], ApcΔ580/+ mice had more than 100 (120 ± 37, n = 11) intestinal tumors at the time of their death (Figure S1).
T5031 3719-3726 JJ denotes Similar
T5033 3727-3729 IN denotes to
T5034 3730-3733 DT denotes the
T5035 3734-3741 NNS denotes results
T5036 3742-3750 VBN denotes reported
T5037 3751-3755 IN denotes with
T5038 3756-3758 DT denotes an
T5040 3759-3772 RB denotes independently
T5041 3773-3782 VBN denotes generated
T5042 3783-3790 NN denotes ApcΔ580
T5044 3790-3791 HYPH denotes /
T5045 3791-3792 SYM denotes +
T5043 3793-3804 JJ denotes conditional
T5046 3805-3810 NN denotes mouse
T5039 3811-3817 NN denotes strain
T5047 3818-3819 -LRB- denotes [
T5048 3819-3821 CD denotes 14
T5049 3821-3822 -RRB- denotes ]
T5050 3822-3824 , denotes ,
T5051 3824-3831 NN denotes ApcΔ580
T5053 3831-3832 HYPH denotes /
T5054 3832-3833 SYM denotes +
T5052 3834-3838 NNS denotes mice
T5032 3839-3842 VBD denotes had
T5055 3843-3847 JJR denotes more
T5057 3848-3852 IN denotes than
T5056 3853-3856 CD denotes 100
T5059 3857-3858 -LRB- denotes (
T5061 3858-3861 CD denotes 120
T5062 3862-3863 SYM denotes ±
T5060 3864-3866 CD denotes 37
T5063 3866-3868 , denotes ,
T5064 3868-3869 NN denotes n
T5066 3870-3871 SYM denotes =
T5065 3872-3874 CD denotes 11
T5067 3874-3875 -RRB- denotes )
T5068 3876-3886 JJ denotes intestinal
T5058 3887-3893 NNS denotes tumors
T5069 3894-3896 IN denotes at
T5070 3897-3900 DT denotes the
T5071 3901-3905 NN denotes time
T5072 3906-3908 IN denotes of
T5073 3909-3914 PRP$ denotes their
T5074 3915-3920 NN denotes death
T5075 3921-3922 -LRB- denotes (
T5077 3922-3928 NN denotes Figure
T5076 3929-3931 NN denotes S1
T5078 3931-3932 -RRB- denotes )
T5079 3932-3933 . denotes .
T5080 3933-4015 sentence denotes Inactivation of wild-type Apc is an important prerequisite for tumor development.
T5081 3934-3946 NN denotes Inactivation
T5083 3947-3949 IN denotes of
T5084 3950-3954 JJ denotes wild
T5086 3954-3955 HYPH denotes -
T5085 3955-3959 NN denotes type
T5087 3960-3963 NN denotes Apc
T5082 3964-3966 VBZ denotes is
T5088 3967-3969 DT denotes an
T5090 3970-3979 JJ denotes important
T5089 3980-3992 NN denotes prerequisite
T5091 3993-3996 IN denotes for
T5092 3997-4002 NN denotes tumor
T5093 4003-4014 NN denotes development
T5094 4014-4015 . denotes .
T5095 4015-4276 sentence denotes We analyzed 30 intestinal tumors from ApcΔ580/+ mice by in vitro transcription and translation assay, but none of them showed truncated Apc products (unpublished data), indicating that the most likely mechanism of wild-type Apc inactivation is by allelic loss.
T5096 4016-4018 PRP denotes We
T5097 4019-4027 VBD denotes analyzed
T5098 4028-4030 CD denotes 30
T5100 4031-4041 JJ denotes intestinal
T5099 4042-4048 NNS denotes tumors
T5101 4049-4053 IN denotes from
T5102 4054-4061 JJ denotes ApcΔ580
T5104 4061-4062 HYPH denotes /
T5105 4062-4063 SYM denotes +
T5103 4064-4068 NNS denotes mice
T5106 4069-4071 IN denotes by
T5107 4072-4074 FW denotes in
T5108 4075-4080 FW denotes vitro
T5109 4081-4094 NN denotes transcription
T5110 4095-4098 CC denotes and
T5111 4099-4110 NN denotes translation
T5112 4111-4116 NN denotes assay
T5113 4116-4118 , denotes ,
T5114 4118-4121 CC denotes but
T5115 4122-4126 NN denotes none
T5117 4127-4129 IN denotes of
T5118 4130-4134 PRP denotes them
T5116 4135-4141 VBD denotes showed
T5119 4142-4151 VBN denotes truncated
T5121 4152-4155 NN denotes Apc
T5120 4156-4164 NNS denotes products
T5122 4165-4166 -LRB- denotes (
T5124 4166-4177 JJ denotes unpublished
T5123 4178-4182 NNS denotes data
T5125 4182-4183 -RRB- denotes )
T5126 4183-4185 , denotes ,
T5127 4185-4195 VBG denotes indicating
T5128 4196-4200 IN denotes that
T5130 4201-4204 DT denotes the
T5132 4205-4209 RBS denotes most
T5133 4210-4216 JJ denotes likely
T5131 4217-4226 NN denotes mechanism
T5134 4227-4229 IN denotes of
T5135 4230-4234 JJ denotes wild
T5137 4234-4235 HYPH denotes -
T5136 4235-4239 NN denotes type
T5139 4240-4243 NN denotes Apc
T5138 4244-4256 NN denotes inactivation
T5129 4257-4259 VBZ denotes is
T5140 4260-4262 IN denotes by
T5141 4263-4270 JJ denotes allelic
T5142 4271-4275 NN denotes loss
T5143 4275-4276 . denotes .
T5144 4276-4474 sentence denotes The mutant allele had to be maintained and transmitted through male mice, as ApcΔ580/+ females were frequently not healthy enough to successfully nurse their own pups because of their tumor burden.
T5145 4277-4280 DT denotes The
T5147 4281-4287 JJ denotes mutant
T5146 4288-4294 NN denotes allele
T5148 4295-4298 VBD denotes had
T5149 4299-4301 TO denotes to
T5151 4302-4304 VB denotes be
T5150 4305-4315 VBN denotes maintained
T5152 4316-4319 CC denotes and
T5153 4320-4331 VBN denotes transmitted
T5154 4332-4339 IN denotes through
T5155 4340-4344 JJ denotes male
T5156 4345-4349 NNS denotes mice
T5157 4349-4351 , denotes ,
T5158 4351-4353 IN denotes as
T5160 4354-4361 NN denotes ApcΔ580
T5162 4361-4362 HYPH denotes /
T5163 4362-4363 SYM denotes +
T5161 4364-4371 NNS denotes females
T5159 4372-4376 VBD denotes were
T5164 4377-4387 RB denotes frequently
T5165 4388-4391 RB denotes not
T5166 4392-4399 JJ denotes healthy
T5167 4400-4406 RB denotes enough
T5168 4407-4409 TO denotes to
T5170 4410-4422 RB denotes successfully
T5169 4423-4428 VB denotes nurse
T5171 4429-4434 PRP$ denotes their
T5173 4435-4438 JJ denotes own
T5172 4439-4443 NNS denotes pups
T5174 4444-4451 IN denotes because
T5175 4452-4454 IN denotes of
T5176 4455-4460 PRP$ denotes their
T5178 4461-4466 NN denotes tumor
T5177 4467-4473 NN denotes burden
T5179 4473-4474 . denotes .
T5180 4474-4540 sentence denotes ApcCKO/+ mice were intercrossed to generate ApcCKO/CKO offspring.
T5181 4475-4481 NN denotes ApcCKO
T5183 4481-4482 HYPH denotes /
T5184 4482-4483 SYM denotes +
T5182 4484-4488 NNS denotes mice
T5186 4489-4493 VBD denotes were
T5185 4494-4506 VBN denotes intercrossed
T5187 4507-4509 TO denotes to
T5188 4510-4518 VB denotes generate
T5189 4519-4525 NN denotes ApcCKO
T5191 4525-4526 HYPH denotes /
T5190 4526-4529 NN denotes CKO
T5192 4530-4539 NN denotes offspring
T5193 4539-4540 . denotes .
T5194 4540-4633 sentence denotes Approximately one-quarter of the offspring (17 of 81) were homozygous for the ApcCKO allele.
T5195 4541-4554 RB denotes Approximately
T5196 4555-4558 CD denotes one
T5198 4558-4559 HYPH denotes -
T5199 4559-4566 JJ denotes quarter
T5200 4567-4569 IN denotes of
T5201 4570-4573 DT denotes the
T5202 4574-4583 NN denotes offspring
T5203 4584-4585 -LRB- denotes (
T5204 4585-4587 CD denotes 17
T5205 4588-4590 IN denotes of
T5206 4591-4593 CD denotes 81
T5207 4593-4594 -RRB- denotes )
T5197 4595-4599 VBD denotes were
T5208 4600-4610 JJ denotes homozygous
T5209 4611-4614 IN denotes for
T5210 4615-4618 DT denotes the
T5212 4619-4625 NN denotes ApcCKO
T5211 4626-4632 NN denotes allele
T5213 4632-4633 . denotes .
T5214 4633-4786 sentence denotes These mice as well as heterozygous mice for ApcCKO allele are normal, showing no differences in their survival to the wild-type littermates (Figure 1C).
T5215 4634-4639 DT denotes These
T5216 4640-4644 NNS denotes mice
T5218 4645-4647 RB denotes as
T5220 4648-4652 RB denotes well
T5219 4653-4655 IN denotes as
T5221 4656-4668 JJ denotes heterozygous
T5222 4669-4673 NNS denotes mice
T5223 4674-4677 IN denotes for
T5224 4678-4684 NN denotes ApcCKO
T5225 4685-4691 NN denotes allele
T5217 4692-4695 VBP denotes are
T5226 4696-4702 JJ denotes normal
T5227 4702-4704 , denotes ,
T5228 4704-4711 VBG denotes showing
T5229 4712-4714 DT denotes no
T5230 4715-4726 NNS denotes differences
T5231 4727-4729 IN denotes in
T5232 4730-4735 PRP$ denotes their
T5233 4736-4744 NN denotes survival
T5234 4745-4747 IN denotes to
T5235 4748-4751 DT denotes the
T5237 4752-4756 JJ denotes wild
T5239 4756-4757 HYPH denotes -
T5238 4757-4761 NN denotes type
T5236 4762-4773 NNS denotes littermates
T5240 4774-4775 -LRB- denotes (
T5242 4775-4781 NN denotes Figure
T5241 4782-4784 NN denotes 1C
T5243 4784-4785 -RRB- denotes )
T5244 4785-4786 . denotes .
T5245 4786-4919 sentence denotes We tested whether our ApcCKO allele can compliment the wild-type allele by crossing the ApcCKO/CKO female with ApcΔ580/+ male mouse.
T5246 4787-4789 PRP denotes We
T5247 4790-4796 VBD denotes tested
T5248 4797-4804 IN denotes whether
T5250 4805-4808 PRP$ denotes our
T5252 4809-4815 NN denotes ApcCKO
T5251 4816-4822 NN denotes allele
T5253 4823-4826 MD denotes can
T5249 4827-4837 VB denotes compliment
T5254 4838-4841 DT denotes the
T5256 4842-4846 JJ denotes wild
T5258 4846-4847 HYPH denotes -
T5257 4847-4851 NN denotes type
T5255 4852-4858 NN denotes allele
T5259 4859-4861 IN denotes by
T5260 4862-4870 VBG denotes crossing
T5261 4871-4874 DT denotes the
T5263 4875-4881 NN denotes ApcCKO
T5265 4881-4882 HYPH denotes /
T5264 4882-4885 NN denotes CKO
T5262 4886-4892 NN denotes female
T5266 4893-4897 IN denotes with
T5267 4898-4905 NN denotes ApcΔ580
T5269 4905-4906 HYPH denotes /
T5270 4906-4907 SYM denotes +
T5271 4908-4912 JJ denotes male
T5268 4913-4918 NN denotes mouse
T5272 4918-4919 . denotes .
T5273 4919-5127 sentence denotes The resultant ApcCKO/Δ580 offspring were viable and born in the Mendelian ratio, suggesting that the presence of loxP sites in introns flanking exon 14 have no adverse effect on the function of the Apc gene.
T5274 4920-4923 DT denotes The
T5276 4924-4933 JJ denotes resultant
T5277 4934-4940 NN denotes ApcCKO
T5279 4940-4941 HYPH denotes /
T5278 4941-4945 NN denotes Δ580
T5275 4946-4955 NN denotes offspring
T5280 4956-4960 VBD denotes were
T5281 4961-4967 JJ denotes viable
T5282 4968-4971 CC denotes and
T5283 4972-4976 VBN denotes born
T5284 4977-4979 IN denotes in
T5285 4980-4983 DT denotes the
T5287 4984-4993 JJ denotes Mendelian
T5286 4994-4999 NN denotes ratio
T5288 4999-5001 , denotes ,
T5289 5001-5011 VBG denotes suggesting
T5290 5012-5016 IN denotes that
T5292 5017-5020 DT denotes the
T5293 5021-5029 NN denotes presence
T5294 5030-5032 IN denotes of
T5295 5033-5037 NN denotes loxP
T5296 5038-5043 NNS denotes sites
T5297 5044-5046 IN denotes in
T5298 5047-5054 NNS denotes introns
T5299 5055-5063 VBG denotes flanking
T5300 5064-5068 NN denotes exon
T5301 5069-5071 CD denotes 14
T5291 5072-5076 VBP denotes have
T5302 5077-5079 DT denotes no
T5304 5080-5087 JJ denotes adverse
T5303 5088-5094 NN denotes effect
T5305 5095-5097 IN denotes on
T5306 5098-5101 DT denotes the
T5307 5102-5110 NN denotes function
T5308 5111-5113 IN denotes of
T5309 5114-5117 DT denotes the
T5311 5118-5121 NN denotes Apc
T5310 5122-5126 NN denotes gene
T5312 5126-5127 . denotes .
T7674 5129-5132 NN denotes K14
T7676 5132-5133 HYPH denotes -
T7675 5133-5139 VBN denotes Driven
T7677 5140-5144 NN denotes Loss
T7679 5145-5147 IN denotes of
T7680 5148-5151 NN denotes Apc
T7678 5152-5159 VBZ denotes Results
T7681 5160-5162 IN denotes in
T7682 5163-5169 JJ denotes Severe
T7684 5170-5176 NN denotes Growth
T7683 5177-5188 NN denotes Retardation
T7685 5189-5192 CC denotes and
T7686 5193-5198 JJ denotes Early
T7687 5199-5208 NN denotes Lethality
T7688 5208-5372 sentence denotes To introduce the mutation of Apc into cells expressing K14, we crossed WW6 ES cell–derived [15] ApcCKO/+ mice with K14-cre recombinase mice in FVB background [16].
T7689 5209-5211 TO denotes To
T7690 5212-5221 VB denotes introduce
T7692 5222-5225 DT denotes the
T7693 5226-5234 NN denotes mutation
T7694 5235-5237 IN denotes of
T7695 5238-5241 NN denotes Apc
T7696 5242-5246 IN denotes into
T7697 5247-5252 NNS denotes cells
T7698 5253-5263 VBG denotes expressing
T7699 5264-5267 NN denotes K14
T7700 5267-5269 , denotes ,
T7701 5269-5271 PRP denotes we
T7691 5272-5279 VBD denotes crossed
T7702 5280-5283 NN denotes WW6
T7704 5284-5286 NN denotes ES
T7705 5287-5291 NN denotes cell
T7707 5291-5292 HYPH denotes –
T7706 5292-5299 VBN denotes derived
T7708 5300-5301 -LRB- denotes [
T7709 5301-5303 CD denotes 15
T7710 5303-5304 -RRB- denotes ]
T7711 5305-5311 NN denotes ApcCKO
T7712 5311-5312 HYPH denotes /
T7713 5312-5313 SYM denotes +
T7703 5314-5318 NNS denotes mice
T7714 5319-5323 IN denotes with
T7715 5324-5327 NN denotes K14
T7717 5327-5328 HYPH denotes -
T7716 5328-5331 NN denotes cre
T7719 5332-5343 NN denotes recombinase
T7718 5344-5348 NNS denotes mice
T7720 5349-5351 IN denotes in
T7721 5352-5355 NN denotes FVB
T7722 5356-5366 NN denotes background
T7723 5367-5368 -LRB- denotes [
T7724 5368-5370 CD denotes 16
T7725 5370-5371 -RRB- denotes ]
T7726 5371-5372 . denotes .
T7727 5372-5443 sentence denotes The K14-cre; ApcCKO/+ mice were normal in appearance and were fertile.
T7728 5373-5376 DT denotes The
T7730 5377-5380 NN denotes K14
T7732 5380-5381 HYPH denotes -
T7731 5381-5384 NN denotes cre
T7733 5384-5385 : denotes ;
T7734 5386-5392 NN denotes ApcCKO
T7735 5392-5393 HYPH denotes /
T7736 5393-5394 SYM denotes +
T7729 5395-5399 NNS denotes mice
T7737 5400-5404 VBD denotes were
T7738 5405-5411 JJ denotes normal
T7739 5412-5414 IN denotes in
T7740 5415-5425 NN denotes appearance
T7741 5426-5429 CC denotes and
T7742 5430-5434 VBD denotes were
T7743 5435-5442 JJ denotes fertile
T7744 5442-5443 . denotes .
T7745 5443-5585 sentence denotes K14-cre; ApcCKO/+ males were crossed to ApcCKO/CKO females to avoid the potential deleter effect in oocytes of K14-cre–positive females [17].
T7746 5444-5447 NN denotes K14
T7748 5447-5448 HYPH denotes -
T7747 5448-5451 NN denotes cre
T7750 5451-5452 : denotes ;
T7751 5453-5459 NN denotes ApcCKO
T7752 5459-5460 HYPH denotes /
T7753 5460-5461 SYM denotes +
T7749 5462-5467 NNS denotes males
T7755 5468-5472 VBD denotes were
T7754 5473-5480 VBN denotes crossed
T7756 5481-5483 IN denotes to
T7757 5484-5490 NN denotes ApcCKO
T7759 5490-5491 HYPH denotes /
T7758 5491-5494 NN denotes CKO
T7760 5495-5502 NNS denotes females
T7761 5503-5505 TO denotes to
T7762 5506-5511 VB denotes avoid
T7763 5512-5515 DT denotes the
T7765 5516-5525 JJ denotes potential
T7766 5526-5533 NN denotes deleter
T7764 5534-5540 NN denotes effect
T7767 5541-5543 IN denotes in
T7768 5544-5551 NNS denotes oocytes
T7769 5552-5554 IN denotes of
T7770 5555-5558 NN denotes K14
T7772 5558-5559 HYPH denotes -
T7771 5559-5562 NN denotes cre
T7774 5562-5563 HYPH denotes –
T7773 5563-5571 JJ denotes positive
T7775 5572-5579 NNS denotes females
T7776 5580-5581 -LRB- denotes [
T7777 5581-5583 CD denotes 17
T7778 5583-5584 -RRB- denotes ]
T7779 5584-5585 . denotes .
T7780 5585-5785 sentence denotes The mice were intercrossed thereafter for maintenance; hence, the mice used for analysis were in a mixed background of FVB, 129/S, and C57BL/6 in similar proportions, with minimal contribution of SJ.
T7781 5586-5589 DT denotes The
T7782 5590-5594 NNS denotes mice
T7784 5595-5599 VBD denotes were
T7783 5600-5612 VBN denotes intercrossed
T7786 5613-5623 RB denotes thereafter
T7787 5624-5627 IN denotes for
T7788 5628-5639 NN denotes maintenance
T7789 5639-5640 : denotes ;
T7790 5641-5646 RB denotes hence
T7791 5646-5648 , denotes ,
T7792 5648-5651 DT denotes the
T7793 5652-5656 NNS denotes mice
T7794 5657-5661 VBN denotes used
T7795 5662-5665 IN denotes for
T7796 5666-5674 NN denotes analysis
T7785 5675-5679 VBD denotes were
T7797 5680-5682 IN denotes in
T7798 5683-5684 DT denotes a
T7800 5685-5690 VBN denotes mixed
T7799 5691-5701 NN denotes background
T7801 5702-5704 IN denotes of
T7802 5705-5708 NN denotes FVB
T7803 5708-5710 , denotes ,
T7804 5710-5713 CD denotes 129
T7806 5713-5714 HYPH denotes /
T7805 5714-5715 NN denotes S
T7807 5715-5717 , denotes ,
T7808 5717-5720 CC denotes and
T7809 5721-5726 NN denotes C57BL
T7810 5726-5727 HYPH denotes /
T7811 5727-5728 CD denotes 6
T7812 5729-5731 IN denotes in
T7813 5732-5739 JJ denotes similar
T7814 5740-5751 NNS denotes proportions
T7815 5751-5753 , denotes ,
T7816 5753-5757 IN denotes with
T7817 5758-5765 JJ denotes minimal
T7818 5766-5778 NN denotes contribution
T7819 5779-5781 IN denotes of
T7820 5782-5784 NN denotes SJ
T7821 5784-5785 . denotes .
T7822 5785-6091 sentence denotes The K14 promoter is a commonly used epidermal cell promoter because of its expression by the mitotically active cells of the epidermis and its appendages in mature skin [18], but most notably it is active in embryonic ectoderm as early as the single layered ectodermal stage of embryonic day (E) 9.5 [19].
T7823 5786-5789 DT denotes The
T7825 5790-5793 NN denotes K14
T7824 5794-5802 NN denotes promoter
T7826 5803-5805 VBZ denotes is
T7827 5806-5807 DT denotes a
T7829 5808-5816 RB denotes commonly
T7830 5817-5821 VBN denotes used
T7831 5822-5831 JJ denotes epidermal
T7832 5832-5836 NN denotes cell
T7828 5837-5845 NN denotes promoter
T7833 5846-5853 IN denotes because
T7834 5854-5856 IN denotes of
T7835 5857-5860 PRP$ denotes its
T7836 5861-5871 NN denotes expression
T7837 5872-5874 IN denotes by
T7838 5875-5878 DT denotes the
T7840 5879-5890 RB denotes mitotically
T7841 5891-5897 JJ denotes active
T7839 5898-5903 NNS denotes cells
T7842 5904-5906 IN denotes of
T7843 5907-5910 DT denotes the
T7844 5911-5920 NN denotes epidermis
T7845 5921-5924 CC denotes and
T7846 5925-5928 PRP$ denotes its
T7847 5929-5939 NNS denotes appendages
T7848 5940-5942 IN denotes in
T7849 5943-5949 JJ denotes mature
T7850 5950-5954 NN denotes skin
T7851 5955-5956 -LRB- denotes [
T7852 5956-5958 CD denotes 18
T7853 5958-5959 -RRB- denotes ]
T7854 5959-5961 , denotes ,
T7855 5961-5964 CC denotes but
T7856 5965-5969 RBS denotes most
T7857 5970-5977 RB denotes notably
T7859 5978-5980 PRP denotes it
T7858 5981-5983 VBZ denotes is
T7860 5984-5990 JJ denotes active
T7861 5991-5993 IN denotes in
T7862 5994-6003 JJ denotes embryonic
T7863 6004-6012 NN denotes ectoderm
T7864 6013-6015 RB denotes as
T7865 6016-6021 RB denotes early
T7866 6022-6024 IN denotes as
T7867 6025-6028 DT denotes the
T7869 6029-6035 JJ denotes single
T7870 6036-6043 VBN denotes layered
T7871 6044-6054 JJ denotes ectodermal
T7868 6055-6060 NN denotes stage
T7872 6061-6063 IN denotes of
T7873 6064-6073 JJ denotes embryonic
T7874 6074-6077 NN denotes day
T7875 6078-6079 -LRB- denotes (
T7876 6079-6080 NN denotes E
T7877 6080-6081 -RRB- denotes )
T7878 6082-6085 CD denotes 9.5
T7879 6086-6087 -LRB- denotes [
T7880 6087-6089 CD denotes 19
T7881 6089-6090 -RRB- denotes ]
T7882 6090-6091 . denotes .
T7883 6091-6208 sentence denotes A restricted expression of K14 is also found in thymic epithelial cells (TECs) in the medulla of normal thymus [20].
T7884 6092-6093 DT denotes A
T7886 6094-6104 JJ denotes restricted
T7885 6105-6115 NN denotes expression
T7888 6116-6118 IN denotes of
T7889 6119-6122 NN denotes K14
T7890 6123-6125 VBZ denotes is
T7891 6126-6130 RB denotes also
T7887 6131-6136 VBN denotes found
T7892 6137-6139 IN denotes in
T7893 6140-6146 JJ denotes thymic
T7895 6147-6157 JJ denotes epithelial
T7894 6158-6163 NNS denotes cells
T7896 6164-6165 -LRB- denotes (
T7897 6165-6169 NNS denotes TECs
T7898 6169-6170 -RRB- denotes )
T7899 6171-6173 IN denotes in
T7900 6174-6177 DT denotes the
T7901 6178-6185 NN denotes medulla
T7902 6186-6188 IN denotes of
T7903 6189-6195 JJ denotes normal
T7904 6196-6202 NN denotes thymus
T7905 6203-6204 -LRB- denotes [
T7906 6204-6206 CD denotes 20
T7907 6206-6207 -RRB- denotes ]
T7908 6207-6208 . denotes .
T7909 6208-6340 sentence denotes We genotyped a total of 458 pups (8–10 d old) from 67 litters resulting from crosses between K14-cre; ApcCKO/+ and ApcCKO/CKO mice.
T7910 6209-6211 PRP denotes We
T7911 6212-6221 VBD denotes genotyped
T7912 6222-6223 DT denotes a
T7914 6224-6229 NN denotes total
T7915 6230-6232 IN denotes of
T7913 6233-6236 CD denotes 458
T7916 6237-6241 NNS denotes pups
T7917 6242-6243 -LRB- denotes (
T7919 6243-6244 CD denotes 8
T7921 6244-6245 SYM denotes –
T7920 6245-6247 CD denotes 10
T7922 6248-6249 NNS denotes d
T7918 6250-6253 JJ denotes old
T7923 6253-6254 -RRB- denotes )
T7924 6255-6259 IN denotes from
T7925 6260-6262 CD denotes 67
T7926 6263-6270 NNS denotes litters
T7927 6271-6280 VBG denotes resulting
T7928 6281-6285 IN denotes from
T7929 6286-6293 NNS denotes crosses
T7930 6294-6301 IN denotes between
T7931 6302-6305 NN denotes K14
T7933 6305-6306 HYPH denotes -
T7932 6306-6309 NN denotes cre
T7935 6309-6310 : denotes ;
T7936 6311-6317 NN denotes ApcCKO
T7937 6317-6318 HYPH denotes /
T7938 6318-6319 SYM denotes +
T7939 6320-6323 CC denotes and
T7940 6324-6330 NN denotes ApcCKO
T7942 6330-6331 HYPH denotes /
T7941 6331-6334 NN denotes CKO
T7934 6335-6339 NNS denotes mice
T7943 6339-6340 . denotes .
T7944 6340-6557 sentence denotes The mutant mice of the genotype K14-cre; ApcCKO/CKO (hereafter, KA mutant) were born, but the observed frequency of KA mutants was much less than expected (78 of 458 [17.0%]; p < 0.0005 Chi-square analysis, Table 1).
T7945 6341-6344 DT denotes The
T7947 6345-6351 JJ denotes mutant
T7946 6352-6356 NNS denotes mice
T7949 6357-6359 IN denotes of
T7950 6360-6363 DT denotes the
T7951 6364-6372 NN denotes genotype
T7952 6373-6376 NN denotes K14
T7954 6376-6377 HYPH denotes -
T7953 6377-6380 NN denotes cre
T7955 6380-6381 : denotes ;
T7956 6382-6388 NN denotes ApcCKO
T7958 6388-6389 HYPH denotes /
T7957 6389-6392 NN denotes CKO
T7959 6393-6394 -LRB- denotes (
T7961 6394-6403 RB denotes hereafter
T7962 6403-6405 , denotes ,
T7963 6405-6407 NN denotes KA
T7960 6408-6414 NN denotes mutant
T7964 6414-6415 -RRB- denotes )
T7965 6416-6420 VBD denotes were
T7948 6421-6425 VBN denotes born
T7966 6425-6427 , denotes ,
T7967 6427-6430 CC denotes but
T7968 6431-6434 DT denotes the
T7970 6435-6443 VBN denotes observed
T7969 6444-6453 NN denotes frequency
T7972 6454-6456 IN denotes of
T7973 6457-6459 NN denotes KA
T7974 6460-6467 NNS denotes mutants
T7971 6468-6471 VBD denotes was
T7975 6472-6476 RB denotes much
T7976 6477-6481 JJR denotes less
T7977 6482-6486 IN denotes than
T7978 6487-6495 VBN denotes expected
T7979 6496-6497 -LRB- denotes (
T7981 6497-6499 CD denotes 78
T7982 6500-6502 IN denotes of
T7983 6503-6506 CD denotes 458
T7984 6507-6508 -LRB- denotes [
T7986 6508-6512 CD denotes 17.0
T7985 6512-6513 NN denotes %
T7987 6513-6514 -RRB- denotes ]
T7988 6514-6515 : denotes ;
T7989 6516-6517 NN denotes p
T7991 6518-6519 SYM denotes <
T7992 6520-6526 CD denotes 0.0005
T7993 6527-6530 NN denotes Chi
T7995 6530-6531 HYPH denotes -
T7994 6531-6537 NN denotes square
T7990 6538-6546 NN denotes analysis
T7996 6546-6548 , denotes ,
T7980 6548-6553 NN denotes Table
T7997 6554-6555 CD denotes 1
T7998 6555-6556 -RRB- denotes )
T7999 6556-6557 . denotes .
T8000 6557-6720 sentence denotes To assess the basis for the neonatal lethality of KA mutants, we monitored three litters from birth to weaning by measuring the body weight of each pup every day.
T8001 6558-6560 TO denotes To
T8002 6561-6567 VB denotes assess
T8004 6568-6571 DT denotes the
T8005 6572-6577 NN denotes basis
T8006 6578-6581 IN denotes for
T8007 6582-6585 DT denotes the
T8009 6586-6594 JJ denotes neonatal
T8008 6595-6604 NN denotes lethality
T8010 6605-6607 IN denotes of
T8011 6608-6610 NN denotes KA
T8012 6611-6618 NNS denotes mutants
T8013 6618-6620 , denotes ,
T8014 6620-6622 PRP denotes we
T8003 6623-6632 VBD denotes monitored
T8015 6633-6638 CD denotes three
T8016 6639-6646 NNS denotes litters
T8017 6647-6651 IN denotes from
T8018 6652-6657 NN denotes birth
T8019 6658-6660 IN denotes to
T8020 6661-6668 VBG denotes weaning
T8021 6669-6671 IN denotes by
T8022 6672-6681 VBG denotes measuring
T8023 6682-6685 DT denotes the
T8025 6686-6690 NN denotes body
T8024 6691-6697 NN denotes weight
T8026 6698-6700 IN denotes of
T8027 6701-6705 DT denotes each
T8028 6706-6709 NN denotes pup
T8029 6710-6715 DT denotes every
T8030 6716-6719 NN denotes day
T8031 6719-6720 . denotes .
T8032 6720-6912 sentence denotes A total of 25 pups were born from three litters, of which 7 (28%) were confirmed to be K14-cre; ApcCKO/CKO by genotyping, indicating that KA mutants were born in the expected Mendelian ratio.
T8033 6721-6722 DT denotes A
T8035 6723-6728 NN denotes total
T8036 6729-6731 IN denotes of
T8034 6732-6734 CD denotes 25
T8037 6735-6739 NNS denotes pups
T8039 6740-6744 VBD denotes were
T8038 6745-6749 VBN denotes born
T8040 6750-6754 IN denotes from
T8041 6755-6760 CD denotes three
T8042 6761-6768 NNS denotes litters
T8043 6768-6770 , denotes ,
T8044 6770-6772 IN denotes of
T8046 6773-6778 WDT denotes which
T8047 6779-6780 CD denotes 7
T8048 6781-6782 -LRB- denotes (
T8050 6782-6784 CD denotes 28
T8049 6784-6785 NN denotes %
T8051 6785-6786 -RRB- denotes )
T8052 6787-6791 VBD denotes were
T8045 6792-6801 VBN denotes confirmed
T8053 6802-6804 TO denotes to
T8054 6805-6807 VB denotes be
T8055 6808-6811 NN denotes K14
T8057 6811-6812 HYPH denotes -
T8056 6812-6815 NN denotes cre
T8058 6815-6816 : denotes ;
T8059 6817-6823 NN denotes ApcCKO
T8061 6823-6824 HYPH denotes /
T8060 6824-6827 NN denotes CKO
T8062 6828-6830 IN denotes by
T8063 6831-6841 NN denotes genotyping
T8064 6841-6843 , denotes ,
T8065 6843-6853 VBG denotes indicating
T8066 6854-6858 IN denotes that
T8068 6859-6861 NN denotes KA
T8069 6862-6869 NNS denotes mutants
T8070 6870-6874 VBD denotes were
T8067 6875-6879 VBN denotes born
T8071 6880-6882 IN denotes in
T8072 6883-6886 DT denotes the
T8074 6887-6895 VBN denotes expected
T8075 6896-6905 JJ denotes Mendelian
T8073 6906-6911 NN denotes ratio
T8076 6911-6912 . denotes .
T8077 6912-7067 sentence denotes The KA mutant pups were nursed normally, and there was milk in their stomachs during the first 2 or 3 d after birth, but they failed to thrive (Figure 2).
T8078 6913-6916 DT denotes The
T8080 6917-6919 NN denotes KA
T8081 6920-6926 JJ denotes mutant
T8079 6927-6931 NNS denotes pups
T8083 6932-6936 VBD denotes were
T8082 6937-6943 VBN denotes nursed
T8084 6944-6952 RB denotes normally
T8085 6952-6954 , denotes ,
T8086 6954-6957 CC denotes and
T8087 6958-6963 EX denotes there
T8088 6964-6967 VBD denotes was
T8089 6968-6972 NN denotes milk
T8090 6973-6975 IN denotes in
T8091 6976-6981 PRP$ denotes their
T8092 6982-6990 NNS denotes stomachs
T8093 6991-6997 IN denotes during
T8094 6998-7001 DT denotes the
T8096 7002-7007 JJ denotes first
T8097 7008-7009 CD denotes 2
T8098 7010-7012 CC denotes or
T8099 7013-7014 CD denotes 3
T8095 7015-7016 NNS denotes d
T8100 7017-7022 IN denotes after
T8101 7023-7028 NN denotes birth
T8102 7028-7030 , denotes ,
T8103 7030-7033 CC denotes but
T8104 7034-7038 PRP denotes they
T8105 7039-7045 VBD denotes failed
T8106 7046-7048 TO denotes to
T8107 7049-7055 VB denotes thrive
T8108 7056-7057 -LRB- denotes (
T8109 7057-7063 NN denotes Figure
T8110 7064-7065 CD denotes 2
T8111 7065-7066 -RRB- denotes )
T8112 7066-7067 . denotes .
T8113 7067-7249 sentence denotes By postnatal day (P) 8–10, at the time of genotyping, many KA mutant pups were considerably smaller than their littermates (Figure 2B–2F) and some have died at or prior to this age.
T8114 7068-7070 IN denotes By
T8116 7071-7080 JJ denotes postnatal
T8117 7081-7084 NN denotes day
T8118 7085-7086 -LRB- denotes (
T8119 7086-7087 NN denotes P
T8120 7087-7088 -RRB- denotes )
T8121 7089-7090 CD denotes 8
T8122 7090-7091 SYM denotes –
T8123 7091-7093 CD denotes 10
T8124 7093-7095 , denotes ,
T8125 7095-7097 IN denotes at
T8126 7098-7101 DT denotes the
T8127 7102-7106 NN denotes time
T8128 7107-7109 IN denotes of
T8129 7110-7120 NN denotes genotyping
T8130 7120-7122 , denotes ,
T8131 7122-7126 JJ denotes many
T8133 7127-7129 NN denotes KA
T8134 7130-7136 JJ denotes mutant
T8132 7137-7141 NNS denotes pups
T8115 7142-7146 VBD denotes were
T8135 7147-7159 RB denotes considerably
T8136 7160-7167 JJR denotes smaller
T8137 7168-7172 IN denotes than
T8138 7173-7178 PRP$ denotes their
T8139 7179-7190 NNS denotes littermates
T8140 7191-7192 -LRB- denotes (
T8142 7192-7198 NN denotes Figure
T8141 7199-7201 NN denotes 2B
T8143 7201-7202 SYM denotes –
T8144 7202-7204 NN denotes 2F
T8145 7204-7205 -RRB- denotes )
T8146 7206-7209 CC denotes and
T8147 7210-7214 DT denotes some
T8149 7215-7219 VBP denotes have
T8148 7220-7224 VBN denotes died
T8150 7225-7227 IN denotes at
T8151 7228-7230 CC denotes or
T8152 7231-7236 JJ denotes prior
T8153 7237-7239 IN denotes to
T8154 7240-7244 DT denotes this
T8155 7245-7248 NN denotes age
T8156 7248-7249 . denotes .
T8157 7249-7293 sentence denotes None of KA mutants survived to weaning age.
T8158 7250-7254 NN denotes None
T8160 7255-7257 IN denotes of
T8161 7258-7260 NN denotes KA
T8162 7261-7268 NNS denotes mutants
T8159 7269-7277 VBD denotes survived
T8163 7278-7280 IN denotes to
T8164 7281-7288 VBG denotes weaning
T8165 7289-7292 NN denotes age
T8166 7292-7293 . denotes .
T8167 7293-8838 sentence denotes Table 1 Genotype Distribution of Progeny from the Matings Figure 2 Postnatal Mortality and Stunted Growth in K14-cre; ApcCKO/CKO Mutant Mice Animals whose genotype is either heterozygous or homozygous for the wild-type Apc allele are referred to as normal (N); those whose genotype are K14-cre; ApcCKO/CKO and show the presence of K14-cre–recombined mutant Apc allele are called mutant (M). (A) Two P3 mutant mice, M1 and M2, and their normal littermates, showing size variation among mutants. (B) P8 mutant mouse (right) and a normal littermate. Note sparseness of hair coat and abnormal ears. (C–D) Vibrissae of whisker pads are short and oddly angled in a P12 mutant mouse (C), relative to control (D). Note the lack of incisors in the mutant. (E) A P17 mutant mouse (right) with its littermate. Its bare forehead, dorsal median line, and abnormal ears are evident. (F) Growth curve of mutants and normal littermates. Mutants exhibit stunted growth, which became more prominent as they aged, and weigh significantly less than littermates from P8 (p < 0.05). (G) Comparison of mutant and normal thymus from P3 mice. The mutant thymus (left) is dramatically smaller for its age compared to the normal littermate (right). The scale bar equals 1 mm. (H) Skeletal preparations of normal (left) and mutant (right), showing differences in development of both incisor (I) and molar (M) teeth. The ability of whole embryos to exclude blue dye was used to examine the epidermal barrier, normally acquired beginning at E16 and complete by E18.5 [21].
T8168 7294-8687 DT denotes Table 1 Genotype Distribution of Progeny from the Matings Figure 2 Postnatal Mortality and Stunted Growth in K14-cre; ApcCKO/CKO Mutant Mice Animals whose genotype is either heterozygous or homozygous for the wild-type Apc allele are referred to as normal (N); those whose genotype are K14-cre; ApcCKO/CKO and show the presence of K14-cre–recombined mutant Apc allele are called mutant (M). (A) Two P3 mutant mice, M1 and M2, and their normal littermates, showing size variation among mutants. (B) P8 mutant mouse (right) and a normal littermate. Note sparseness of hair coat and abnormal ears. (C–D) Vibrissae of whisker pads are short and oddly angled in a P12 mutant mouse (C), relative to control (D). Note the lack of incisors in the mutant. (E) A P17 mutant mouse (right) with its littermate. Its bare forehead, dorsal median line, and abnormal ears are evident. (F) Growth curve of mutants and normal littermates. Mutants exhibit stunted growth, which became more prominent as they aged, and weigh significantly less than littermates from P8 (p < 0.05). (G) Comparison of mutant and normal thymus from P3 mice. The mutant thymus (left) is dramatically smaller for its age compared to the normal littermate (right). The scale bar equals 1 mm. (H) Skeletal preparations of normal (left) and mutant (right), showing differences in development of both incisor (I) and molar (M) teeth. The
T8169 8688-8695 NN denotes ability
T8171 8696-8698 IN denotes of
T8172 8699-8704 JJ denotes whole
T8173 8705-8712 NNS denotes embryos
T8174 8713-8715 TO denotes to
T8175 8716-8723 VB denotes exclude
T8176 8724-8728 JJ denotes blue
T8177 8729-8732 NN denotes dye
T8178 8733-8736 VBD denotes was
T8170 8737-8741 VBN denotes used
T8179 8742-8744 TO denotes to
T8180 8745-8752 VB denotes examine
T8181 8753-8756 DT denotes the
T8183 8757-8766 JJ denotes epidermal
T8182 8767-8774 NN denotes barrier
T8184 8774-8776 , denotes ,
T8185 8776-8784 RB denotes normally
T8186 8785-8793 VBN denotes acquired
T8187 8794-8803 VBG denotes beginning
T8188 8804-8806 IN denotes at
T8189 8807-8810 NN denotes E16
T8190 8811-8814 CC denotes and
T8191 8815-8823 JJ denotes complete
T8192 8824-8826 IN denotes by
T8193 8827-8832 NN denotes E18.5
T8194 8833-8834 -LRB- denotes [
T8195 8834-8836 CD denotes 21
T8196 8836-8837 -RRB- denotes ]
T8197 8837-8838 . denotes .
T8198 8838-8983 sentence denotes Analyses of E17.5–E18.5 KA mutants showed that they were able to exclude blue dye, indicating that the epidermal barrier was intact (Figure S2).
T8199 8839-8847 NNS denotes Analyses
T8201 8848-8850 IN denotes of
T8202 8851-8856 NN denotes E17.5
T8204 8856-8857 SYM denotes –
T8205 8857-8862 NN denotes E18.5
T8206 8863-8865 NN denotes KA
T8203 8866-8873 NNS denotes mutants
T8200 8874-8880 VBD denotes showed
T8207 8881-8885 IN denotes that
T8209 8886-8890 PRP denotes they
T8208 8891-8895 VBD denotes were
T8210 8896-8900 JJ denotes able
T8211 8901-8903 TO denotes to
T8212 8904-8911 VB denotes exclude
T8213 8912-8916 JJ denotes blue
T8214 8917-8920 NN denotes dye
T8215 8920-8922 , denotes ,
T8216 8922-8932 VBG denotes indicating
T8217 8933-8937 IN denotes that
T8219 8938-8941 DT denotes the
T8221 8942-8951 JJ denotes epidermal
T8220 8952-8959 NN denotes barrier
T8218 8960-8963 VBD denotes was
T8222 8964-8970 JJ denotes intact
T8223 8971-8972 -LRB- denotes (
T8225 8972-8978 NN denotes Figure
T8224 8979-8981 NN denotes S2
T8226 8981-8982 -RRB- denotes )
T8227 8982-8983 . denotes .
T8228 8983-9231 sentence denotes At these embryonic ages, there were no differences in size between the mutants and their littermates, but the mutants showed a patch of “birthmark” or dark pigmentation on their foreheads and a dark median line that ran caudally from head to tail.
T8229 8984-8986 IN denotes At
T8231 8987-8992 DT denotes these
T8233 8993-9002 JJ denotes embryonic
T8232 9003-9007 NNS denotes ages
T8234 9007-9009 , denotes ,
T8235 9009-9014 EX denotes there
T8230 9015-9019 VBD denotes were
T8236 9020-9022 DT denotes no
T8237 9023-9034 NNS denotes differences
T8238 9035-9037 IN denotes in
T8239 9038-9042 NN denotes size
T8240 9043-9050 IN denotes between
T8241 9051-9054 DT denotes the
T8242 9055-9062 NNS denotes mutants
T8243 9063-9066 CC denotes and
T8244 9067-9072 PRP$ denotes their
T8245 9073-9084 NNS denotes littermates
T8246 9084-9086 , denotes ,
T8247 9086-9089 CC denotes but
T8248 9090-9093 DT denotes the
T8249 9094-9101 NNS denotes mutants
T8250 9102-9108 VBD denotes showed
T8251 9109-9110 DT denotes a
T8252 9111-9116 NN denotes patch
T8253 9117-9119 IN denotes of
T8254 9120-9121 `` denotes “
T8255 9121-9130 NN denotes birthmark
T8256 9130-9131 '' denotes ”
T8257 9132-9134 CC denotes or
T8258 9135-9139 JJ denotes dark
T8259 9140-9152 NN denotes pigmentation
T8260 9153-9155 IN denotes on
T8261 9156-9161 PRP$ denotes their
T8262 9162-9171 NNS denotes foreheads
T8263 9172-9175 CC denotes and
T8264 9176-9177 DT denotes a
T8266 9178-9182 JJ denotes dark
T8267 9183-9189 JJ denotes median
T8265 9190-9194 NN denotes line
T8268 9195-9199 WDT denotes that
T8269 9200-9203 VBD denotes ran
T8270 9204-9212 RB denotes caudally
T8271 9213-9217 IN denotes from
T8272 9218-9222 NN denotes head
T8273 9223-9225 IN denotes to
T8274 9226-9230 NN denotes tail
T8275 9230-9231 . denotes .
T8276 9231-9338 sentence denotes Their external ears or pinnae were shriveled in appearance and pigmented compared to those of littermates.
T8277 9232-9237 PRP$ denotes Their
T8279 9238-9246 JJ denotes external
T8278 9247-9251 NNS denotes ears
T8281 9252-9254 CC denotes or
T8282 9255-9261 NNS denotes pinnae
T8280 9262-9266 VBD denotes were
T8283 9267-9276 JJ denotes shriveled
T8284 9277-9279 IN denotes in
T8285 9280-9290 NN denotes appearance
T8286 9291-9294 CC denotes and
T8287 9295-9304 JJ denotes pigmented
T8288 9305-9313 VBN denotes compared
T8289 9314-9316 IN denotes to
T8290 9317-9322 DT denotes those
T8291 9323-9325 IN denotes of
T8292 9326-9337 NNS denotes littermates
T8293 9337-9338 . denotes .
T8294 9338-9481 sentence denotes External characteristics of KA mutants that were evident at E18.5 persisted after birth and became more prominent as they grew (Figure 2A–2F).
T8295 9339-9347 JJ denotes External
T8296 9348-9363 NNS denotes characteristics
T8298 9364-9366 IN denotes of
T8299 9367-9369 NN denotes KA
T8300 9370-9377 NNS denotes mutants
T8301 9378-9382 WDT denotes that
T8302 9383-9387 VBD denotes were
T8303 9388-9395 JJ denotes evident
T8304 9396-9398 IN denotes at
T8305 9399-9404 NN denotes E18.5
T8297 9405-9414 VBD denotes persisted
T8306 9415-9420 IN denotes after
T8307 9421-9426 NN denotes birth
T8308 9427-9430 CC denotes and
T8309 9431-9437 VBD denotes became
T8310 9438-9442 RBR denotes more
T8311 9443-9452 JJ denotes prominent
T8312 9453-9455 IN denotes as
T8314 9456-9460 PRP denotes they
T8313 9461-9465 VBD denotes grew
T8315 9466-9467 -LRB- denotes (
T8317 9467-9473 NN denotes Figure
T8316 9474-9476 NN denotes 2A
T8318 9476-9477 SYM denotes –
T8319 9477-9479 NN denotes 2F
T8320 9479-9480 -RRB- denotes )
T8321 9480-9481 . denotes .
T8322 9481-9541 sentence denotes Growth of pelage hair was generally delayed in the mutants.
T8323 9482-9488 NN denotes Growth
T8325 9489-9491 IN denotes of
T8326 9492-9498 NN denotes pelage
T8327 9499-9503 NN denotes hair
T8328 9504-9507 VBD denotes was
T8329 9508-9517 RB denotes generally
T8324 9518-9525 VBN denotes delayed
T8330 9526-9528 IN denotes in
T8331 9529-9532 DT denotes the
T8332 9533-9540 NNS denotes mutants
T8333 9540-9541 . denotes .
T8334 9541-9696 sentence denotes At around P8, the KA mutants were hairless and had wrinkled skin while their phenotypically normal littermates had a smooth thin coat of hair (Figure 2B).
T8335 9542-9544 IN denotes At
T8337 9545-9551 RB denotes around
T8338 9552-9554 NN denotes P8
T8339 9554-9556 , denotes ,
T8340 9556-9559 DT denotes the
T8342 9560-9562 NN denotes KA
T8341 9563-9570 NNS denotes mutants
T8336 9571-9575 VBD denotes were
T8343 9576-9584 JJ denotes hairless
T8344 9585-9588 CC denotes and
T8345 9589-9592 VBD denotes had
T8346 9593-9601 VBN denotes wrinkled
T8347 9602-9606 NN denotes skin
T8348 9607-9612 IN denotes while
T8350 9613-9618 PRP$ denotes their
T8352 9619-9633 RB denotes phenotypically
T8353 9634-9640 JJ denotes normal
T8351 9641-9652 NNS denotes littermates
T8349 9653-9656 VBD denotes had
T8354 9657-9658 DT denotes a
T8356 9659-9665 JJ denotes smooth
T8357 9666-9670 JJ denotes thin
T8355 9671-9675 NN denotes coat
T8358 9676-9678 IN denotes of
T8359 9679-9683 NN denotes hair
T8360 9684-9685 -LRB- denotes (
T8362 9685-9691 NN denotes Figure
T8361 9692-9694 NN denotes 2B
T8363 9694-9695 -RRB- denotes )
T8364 9695-9696 . denotes .
T8365 9696-9825 sentence denotes At this age, two lower incisors start to erupt in normal littermates and these were absent in the KA mutants (Figure 2C and 2D).
T8366 9697-9699 IN denotes At
T8368 9700-9704 DT denotes this
T8369 9705-9708 NN denotes age
T8370 9708-9710 , denotes ,
T8371 9710-9713 CD denotes two
T8373 9714-9719 JJR denotes lower
T8372 9720-9728 NNS denotes incisors
T8367 9729-9734 VBP denotes start
T8374 9735-9737 TO denotes to
T8375 9738-9743 VB denotes erupt
T8376 9744-9746 IN denotes in
T8377 9747-9753 JJ denotes normal
T8378 9754-9765 NNS denotes littermates
T8379 9766-9769 CC denotes and
T8380 9770-9775 DT denotes these
T8381 9776-9780 VBD denotes were
T8382 9781-9787 JJ denotes absent
T8383 9788-9790 IN denotes in
T8384 9791-9794 DT denotes the
T8386 9795-9797 NN denotes KA
T8385 9798-9805 NNS denotes mutants
T8387 9806-9807 -LRB- denotes (
T8389 9807-9813 NN denotes Figure
T8388 9814-9816 NN denotes 2C
T8390 9817-9820 CC denotes and
T8391 9821-9823 NN denotes 2D
T8392 9823-9824 -RRB- denotes )
T8393 9824-9825 . denotes .
T8394 9825-9980 sentence denotes Animals also tended to be smaller and around P10–P12 displayed abnormally short and misshapen vibrissae and short, shaggy pelage hairs (Figure 2C and 2D).
T8395 9826-9833 NNS denotes Animals
T8397 9834-9838 RB denotes also
T8396 9839-9845 VBD denotes tended
T8398 9846-9848 TO denotes to
T8399 9849-9851 VB denotes be
T8400 9852-9859 JJR denotes smaller
T8401 9860-9863 CC denotes and
T8402 9864-9870 IN denotes around
T8404 9871-9874 NN denotes P10
T8405 9874-9875 SYM denotes –
T8406 9875-9878 NN denotes P12
T8403 9879-9888 VBD denotes displayed
T8407 9889-9899 RB denotes abnormally
T8408 9900-9905 JJ denotes short
T8410 9906-9909 CC denotes and
T8411 9910-9919 JJ denotes misshapen
T8409 9920-9929 NNS denotes vibrissae
T8412 9930-9933 CC denotes and
T8413 9934-9939 JJ denotes short
T8415 9939-9941 , denotes ,
T8416 9941-9947 JJ denotes shaggy
T8417 9948-9954 NN denotes pelage
T8414 9955-9960 NNS denotes hairs
T8418 9961-9962 -LRB- denotes (
T8420 9962-9968 NN denotes Figure
T8419 9969-9971 NN denotes 2C
T8421 9972-9975 CC denotes and
T8422 9976-9978 NN denotes 2D
T8423 9978-9979 -RRB- denotes )
T8424 9979-9980 . denotes .
T8425 9980-10119 sentence denotes Development of thick ridges in their skin, particularly around the ears, eyelids, forehead, nose, and paws, became noticeable (Figure 2E).
T8426 9981-9992 NN denotes Development
T8428 9993-9995 IN denotes of
T8429 9996-10001 JJ denotes thick
T8430 10002-10008 NNS denotes ridges
T8431 10009-10011 IN denotes in
T8432 10012-10017 PRP$ denotes their
T8433 10018-10022 NN denotes skin
T8434 10022-10024 , denotes ,
T8435 10024-10036 RB denotes particularly
T8436 10037-10043 IN denotes around
T8437 10044-10047 DT denotes the
T8438 10048-10052 NNS denotes ears
T8439 10052-10054 , denotes ,
T8440 10054-10061 NNS denotes eyelids
T8441 10061-10063 , denotes ,
T8442 10063-10071 NN denotes forehead
T8443 10071-10073 , denotes ,
T8444 10073-10077 NN denotes nose
T8445 10077-10079 , denotes ,
T8446 10079-10082 CC denotes and
T8447 10083-10087 NNS denotes paws
T8448 10087-10089 , denotes ,
T8427 10089-10095 VBD denotes became
T8449 10096-10106 JJ denotes noticeable
T8450 10107-10108 -LRB- denotes (
T8452 10108-10114 NN denotes Figure
T8451 10115-10117 NN denotes 2E
T8453 10117-10118 -RRB- denotes )
T8454 10118-10119 . denotes .
T8455 10119-10194 sentence denotes These regions looked scaly, and these animals hardly kept their eyes open.
T8456 10120-10125 DT denotes These
T8457 10126-10133 NNS denotes regions
T8458 10134-10140 VBD denotes looked
T8459 10141-10146 JJ denotes scaly
T8460 10146-10148 , denotes ,
T8461 10148-10151 CC denotes and
T8462 10152-10157 DT denotes these
T8463 10158-10165 NNS denotes animals
T8465 10166-10172 RB denotes hardly
T8464 10173-10177 VBD denotes kept
T8466 10178-10183 PRP$ denotes their
T8467 10184-10188 NNS denotes eyes
T8468 10189-10193 JJ denotes open
T8469 10193-10194 . denotes .
T8470 10194-10443 sentence denotes In contrast to the normal littermates that consistently increased their body weight with age, surviving KA mutants started to lose weight from P10 onwards; by P16–P17 they were all lethargic, and none of them survived to weaning (Figure 2E and 2F).
T8471 10195-10197 IN denotes In
T8473 10198-10206 NN denotes contrast
T8474 10207-10209 IN denotes to
T8475 10210-10213 DT denotes the
T8477 10214-10220 JJ denotes normal
T8476 10221-10232 NNS denotes littermates
T8478 10233-10237 WDT denotes that
T8480 10238-10250 RB denotes consistently
T8479 10251-10260 VBD denotes increased
T8481 10261-10266 PRP$ denotes their
T8483 10267-10271 NN denotes body
T8482 10272-10278 NN denotes weight
T8484 10279-10283 IN denotes with
T8485 10284-10287 NN denotes age
T8486 10287-10289 , denotes ,
T8487 10289-10298 VBG denotes surviving
T8489 10299-10301 NN denotes KA
T8488 10302-10309 NNS denotes mutants
T8472 10310-10317 VBD denotes started
T8491 10318-10320 TO denotes to
T8492 10321-10325 VB denotes lose
T8493 10326-10332 NN denotes weight
T8494 10333-10337 IN denotes from
T8495 10338-10341 NN denotes P10
T8496 10342-10349 RB denotes onwards
T8497 10349-10350 : denotes ;
T8498 10351-10353 IN denotes by
T8499 10354-10357 NN denotes P16
T8500 10357-10358 SYM denotes –
T8501 10358-10361 NN denotes P17
T8502 10362-10366 PRP denotes they
T8490 10367-10371 VBD denotes were
T8503 10372-10375 RB denotes all
T8504 10376-10385 JJ denotes lethargic
T8505 10385-10387 , denotes ,
T8506 10387-10390 CC denotes and
T8507 10391-10395 NN denotes none
T8509 10396-10398 IN denotes of
T8510 10399-10403 PRP denotes them
T8508 10404-10412 VBD denotes survived
T8511 10413-10415 IN denotes to
T8512 10416-10423 NN denotes weaning
T8513 10424-10425 -LRB- denotes (
T8515 10425-10431 NN denotes Figure
T8514 10432-10434 NN denotes 2E
T8516 10435-10438 CC denotes and
T8517 10439-10441 NN denotes 2F
T8518 10441-10442 -RRB- denotes )
T8519 10442-10443 . denotes .
T8520 10443-10736 sentence denotes At the time of autopsy all the mutants were toothless, without incisors or molars, and their stomachs were consistently small and had no solid food, unlike their age-matched littermates, suggesting that the observed weight loss could be the result of failure to ingest solid food (Figure 2F).
T8521 10444-10446 IN denotes At
T8523 10447-10450 DT denotes the
T8524 10451-10455 NN denotes time
T8525 10456-10458 IN denotes of
T8526 10459-10466 NN denotes autopsy
T8527 10467-10470 PDT denotes all
T8529 10471-10474 DT denotes the
T8528 10475-10482 NNS denotes mutants
T8522 10483-10487 VBD denotes were
T8530 10488-10497 JJ denotes toothless
T8531 10497-10499 , denotes ,
T8532 10499-10506 IN denotes without
T8533 10507-10515 NNS denotes incisors
T8534 10516-10518 CC denotes or
T8535 10519-10525 NNS denotes molars
T8536 10525-10527 , denotes ,
T8537 10527-10530 CC denotes and
T8538 10531-10536 PRP$ denotes their
T8539 10537-10545 NNS denotes stomachs
T8540 10546-10550 VBD denotes were
T8541 10551-10563 RB denotes consistently
T8542 10564-10569 JJ denotes small
T8543 10570-10573 CC denotes and
T8544 10574-10577 VBD denotes had
T8545 10578-10580 DT denotes no
T8547 10581-10586 JJ denotes solid
T8546 10587-10591 NN denotes food
T8548 10591-10593 , denotes ,
T8549 10593-10599 IN denotes unlike
T8550 10600-10605 PRP$ denotes their
T8552 10606-10609 NN denotes age
T8554 10609-10610 HYPH denotes -
T8553 10610-10617 JJ denotes matched
T8551 10618-10629 NNS denotes littermates
T8555 10629-10631 , denotes ,
T8556 10631-10641 VBG denotes suggesting
T8557 10642-10646 IN denotes that
T8559 10647-10650 DT denotes the
T8561 10651-10659 VBN denotes observed
T8562 10660-10666 NN denotes weight
T8560 10667-10671 NN denotes loss
T8563 10672-10677 MD denotes could
T8558 10678-10680 VB denotes be
T8564 10681-10684 DT denotes the
T8565 10685-10691 NN denotes result
T8566 10692-10694 IN denotes of
T8567 10695-10702 NN denotes failure
T8568 10703-10705 TO denotes to
T8569 10706-10712 VB denotes ingest
T8570 10713-10718 JJ denotes solid
T8571 10719-10723 NN denotes food
T8572 10724-10725 -LRB- denotes (
T8574 10725-10731 NN denotes Figure
T8573 10732-10734 NN denotes 2F
T8575 10734-10735 -RRB- denotes )
T8576 10735-10736 . denotes .
T8577 10736-10952 sentence denotes Interestingly, changes in body weights and timing of hair growth varied considerably among mutant pups even if they were from the same litter, whereas those of phenotypically normal littermates tended to be similar.
T8578 10737-10750 RB denotes Interestingly
T8580 10750-10752 , denotes ,
T8581 10752-10759 NNS denotes changes
T8582 10760-10762 IN denotes in
T8583 10763-10767 NN denotes body
T8584 10768-10775 NNS denotes weights
T8585 10776-10779 CC denotes and
T8586 10780-10786 NN denotes timing
T8587 10787-10789 IN denotes of
T8588 10790-10794 NN denotes hair
T8589 10795-10801 NN denotes growth
T8579 10802-10808 VBD denotes varied
T8590 10809-10821 RB denotes considerably
T8591 10822-10827 IN denotes among
T8592 10828-10834 JJ denotes mutant
T8593 10835-10839 NNS denotes pups
T8594 10840-10844 RB denotes even
T8596 10845-10847 IN denotes if
T8597 10848-10852 PRP denotes they
T8595 10853-10857 VBD denotes were
T8598 10858-10862 IN denotes from
T8599 10863-10866 DT denotes the
T8601 10867-10871 JJ denotes same
T8600 10872-10878 NN denotes litter
T8602 10878-10880 , denotes ,
T8603 10880-10887 IN denotes whereas
T8605 10888-10893 DT denotes those
T8606 10894-10896 IN denotes of
T8607 10897-10911 RB denotes phenotypically
T8608 10912-10918 JJ denotes normal
T8609 10919-10930 NNS denotes littermates
T8604 10931-10937 VBD denotes tended
T8610 10938-10940 TO denotes to
T8611 10941-10943 VB denotes be
T8612 10944-10951 JJ denotes similar
T8613 10951-10952 . denotes .
T8614 10952-11138 sentence denotes This difference was also reflected in the variation in timing of death in mutants: some mutant pups were born alive but died within a day or two, some survived close to the weaning age.
T8615 10953-10957 DT denotes This
T8616 10958-10968 NN denotes difference
T8618 10969-10972 VBD denotes was
T8619 10973-10977 RB denotes also
T8617 10978-10987 VBN denotes reflected
T8621 10988-10990 IN denotes in
T8622 10991-10994 DT denotes the
T8623 10995-11004 NN denotes variation
T8624 11005-11007 IN denotes in
T8625 11008-11014 NN denotes timing
T8626 11015-11017 IN denotes of
T8627 11018-11023 NN denotes death
T8628 11024-11026 IN denotes in
T8629 11027-11034 NNS denotes mutants
T8630 11034-11036 : denotes :
T8631 11036-11040 DT denotes some
T8633 11041-11047 JJ denotes mutant
T8632 11048-11052 NNS denotes pups
T8635 11053-11057 VBD denotes were
T8634 11058-11062 VBN denotes born
T8636 11063-11068 JJ denotes alive
T8637 11069-11072 CC denotes but
T8638 11073-11077 VBD denotes died
T8639 11078-11084 IN denotes within
T8640 11085-11086 DT denotes a
T8641 11087-11090 NN denotes day
T8642 11091-11093 CC denotes or
T8643 11094-11097 CD denotes two
T8644 11097-11099 , denotes ,
T8645 11099-11103 DT denotes some
T8620 11104-11112 VBD denotes survived
T8646 11113-11118 RB denotes close
T8647 11119-11121 IN denotes to
T8648 11122-11125 DT denotes the
T8650 11126-11133 JJ denotes weaning
T8649 11134-11137 NN denotes age
T8651 11137-11138 . denotes .
T8652 11138-11267 sentence denotes This variability of the mutant phenotypes suggests possible variation in the timing and efficiency of cre-mediated Apc deletion.
T8653 11139-11143 DT denotes This
T8654 11144-11155 NN denotes variability
T8656 11156-11158 IN denotes of
T8657 11159-11162 DT denotes the
T8659 11163-11169 JJ denotes mutant
T8658 11170-11180 NNS denotes phenotypes
T8655 11181-11189 VBZ denotes suggests
T8660 11190-11198 JJ denotes possible
T8661 11199-11208 NN denotes variation
T8662 11209-11211 IN denotes in
T8663 11212-11215 DT denotes the
T8664 11216-11222 NN denotes timing
T8665 11223-11226 CC denotes and
T8666 11227-11237 NN denotes efficiency
T8667 11238-11240 IN denotes of
T8668 11241-11244 NN denotes cre
T8670 11244-11245 HYPH denotes -
T8669 11245-11253 JJ denotes mediated
T8672 11254-11257 NN denotes Apc
T8671 11258-11266 NN denotes deletion
T8673 11266-11267 . denotes .
T8674 11267-11350 sentence denotes It is possible that the genetic background has a role to play in this variability.
T8675 11268-11270 PRP denotes It
T8676 11271-11273 VBZ denotes is
T8677 11274-11282 JJ denotes possible
T8678 11283-11287 IN denotes that
T8680 11288-11291 DT denotes the
T8682 11292-11299 JJ denotes genetic
T8681 11300-11310 NN denotes background
T8679 11311-11314 VBZ denotes has
T8683 11315-11316 DT denotes a
T8684 11317-11321 NN denotes role
T8685 11322-11324 TO denotes to
T8686 11325-11329 VB denotes play
T8687 11330-11332 IN denotes in
T8688 11333-11337 DT denotes this
T8689 11338-11349 NN denotes variability
T8690 11349-11350 . denotes .
T8691 11350-11566 sentence denotes Gross examination of internal organs also showed that the mutants' thymi were consistently inconspicuous and were very small for their age, whereas those of their littermates were very prominent in size (Figure 2G).
T8692 11351-11356 JJ denotes Gross
T8693 11357-11368 NN denotes examination
T8695 11369-11371 IN denotes of
T8696 11372-11380 JJ denotes internal
T8697 11381-11387 NNS denotes organs
T8698 11388-11392 RB denotes also
T8694 11393-11399 VBD denotes showed
T8699 11400-11404 IN denotes that
T8701 11405-11408 DT denotes the
T8702 11409-11416 NNS denotes mutants
T8704 11416-11417 POS denotes '
T8703 11418-11423 NNS denotes thymi
T8700 11424-11428 VBD denotes were
T8705 11429-11441 RB denotes consistently
T8706 11442-11455 JJ denotes inconspicuous
T8707 11456-11459 CC denotes and
T8708 11460-11464 VBD denotes were
T8709 11465-11469 RB denotes very
T8710 11470-11475 JJ denotes small
T8711 11476-11479 IN denotes for
T8712 11480-11485 PRP$ denotes their
T8713 11486-11489 NN denotes age
T8714 11489-11491 , denotes ,
T8715 11491-11498 IN denotes whereas
T8717 11499-11504 DT denotes those
T8718 11505-11507 IN denotes of
T8719 11508-11513 PRP$ denotes their
T8720 11514-11525 NNS denotes littermates
T8716 11526-11530 VBD denotes were
T8721 11531-11535 RB denotes very
T8722 11536-11545 JJ denotes prominent
T8723 11546-11548 IN denotes in
T8724 11549-11553 NN denotes size
T8725 11554-11555 -LRB- denotes (
T8727 11555-11561 NN denotes Figure
T8726 11562-11564 NN denotes 2G
T8728 11564-11565 -RRB- denotes )
T8729 11565-11566 . denotes .
T8730 11566-11610 sentence denotes This difference was evident as early as P3.
T8731 11567-11571 DT denotes This
T8732 11572-11582 NN denotes difference
T8733 11583-11586 VBD denotes was
T8734 11587-11594 JJ denotes evident
T8735 11595-11597 RB denotes as
T8736 11598-11603 RB denotes early
T8737 11604-11606 IN denotes as
T8738 11607-11609 NN denotes P3
T8739 11609-11610 . denotes .
T8740 11610-11731 sentence denotes Quite frequently mutant thymi in P12–P17 mutant mice also contained black deposits within the tissue (unpublished data).
T8741 11611-11616 RB denotes Quite
T8742 11617-11627 RB denotes frequently
T8744 11628-11634 JJ denotes mutant
T8745 11635-11640 NNS denotes thymi
T8746 11641-11643 IN denotes in
T8747 11644-11647 NN denotes P12
T8749 11647-11648 SYM denotes –
T8750 11648-11651 NN denotes P17
T8751 11652-11658 JJ denotes mutant
T8748 11659-11663 NNS denotes mice
T8752 11664-11668 RB denotes also
T8743 11669-11678 VBD denotes contained
T8753 11679-11684 JJ denotes black
T8754 11685-11693 NNS denotes deposits
T8755 11694-11700 IN denotes within
T8756 11701-11704 DT denotes the
T8757 11705-11711 NN denotes tissue
T8758 11712-11713 -LRB- denotes (
T8760 11713-11724 JJ denotes unpublished
T8759 11725-11729 NNS denotes data
T8761 11729-11730 -RRB- denotes )
T8762 11730-11731 . denotes .
T8763 11731-11868 sentence denotes Mutant mice were also examined for any skeletal abnormalities by preparing skeletal specimens of P16–P17 mice stained with Alizarin red.
T8764 11732-11738 JJ denotes Mutant
T8765 11739-11743 NNS denotes mice
T8767 11744-11748 VBD denotes were
T8768 11749-11753 RB denotes also
T8766 11754-11762 VBN denotes examined
T8769 11763-11766 IN denotes for
T8770 11767-11770 DT denotes any
T8772 11771-11779 JJ denotes skeletal
T8771 11780-11793 NNS denotes abnormalities
T8773 11794-11796 IN denotes by
T8774 11797-11806 VBG denotes preparing
T8775 11807-11815 JJ denotes skeletal
T8776 11816-11825 NNS denotes specimens
T8777 11826-11828 IN denotes of
T8778 11829-11832 NN denotes P16
T8780 11832-11833 SYM denotes –
T8781 11833-11836 NN denotes P17
T8779 11837-11841 NNS denotes mice
T8782 11842-11849 VBN denotes stained
T8783 11850-11854 IN denotes with
T8784 11855-11863 NN denotes Alizarin
T8785 11864-11867 NN denotes red
T8786 11867-11868 . denotes .
T8787 11868-12060 sentence denotes No differences between the normal and KA mutant mice in the mandibular bone can be detected, but the mutant mice lacked or had underdeveloped set of maxillary incisors and molars (Figure 2H).
T8788 11869-11871 DT denotes No
T8789 11872-11883 NNS denotes differences
T8791 11884-11891 IN denotes between
T8792 11892-11895 DT denotes the
T8794 11896-11902 JJ denotes normal
T8795 11903-11906 CC denotes and
T8796 11907-11909 NN denotes KA
T8797 11910-11916 JJ denotes mutant
T8793 11917-11921 NNS denotes mice
T8798 11922-11924 IN denotes in
T8799 11925-11928 DT denotes the
T8801 11929-11939 JJ denotes mandibular
T8800 11940-11944 NN denotes bone
T8802 11945-11948 MD denotes can
T8803 11949-11951 VB denotes be
T8790 11952-11960 VBN denotes detected
T8804 11960-11962 , denotes ,
T8805 11962-11965 CC denotes but
T8806 11966-11969 DT denotes the
T8808 11970-11976 JJ denotes mutant
T8807 11977-11981 NNS denotes mice
T8809 11982-11988 VBD denotes lacked
T8810 11989-11991 CC denotes or
T8811 11992-11995 VBD denotes had
T8812 11996-12010 VBN denotes underdeveloped
T8813 12011-12014 NN denotes set
T8814 12015-12017 IN denotes of
T8815 12018-12027 JJ denotes maxillary
T8816 12028-12036 NNS denotes incisors
T8817 12037-12040 CC denotes and
T8818 12041-12047 NNS denotes molars
T8819 12048-12049 -LRB- denotes (
T8821 12049-12055 NN denotes Figure
T8820 12056-12058 NN denotes 2H
T8822 12058-12059 -RRB- denotes )
T8823 12059-12060 . denotes .
T8824 12060-12111 sentence denotes We detected no other major skeletal abnormalities.
T8825 12061-12063 PRP denotes We
T8826 12064-12072 VBD denotes detected
T8827 12073-12075 DT denotes no
T8829 12076-12081 JJ denotes other
T8830 12082-12087 JJ denotes major
T8831 12088-12096 JJ denotes skeletal
T8828 12097-12110 NNS denotes abnormalities
T8832 12110-12111 . denotes .
T9607 12113-12121 NN denotes Genotype
T9609 12121-12122 HYPH denotes -
T9610 12123-12126 CC denotes and
T9611 12127-12133 NN denotes Tissue
T9612 12133-12134 HYPH denotes -
T9608 12134-12142 JJ denotes Specific
T9613 12143-12153 NN denotes Expression
T9614 12154-12156 IN denotes of
T9615 12157-12160 DT denotes the
T9617 12161-12170 VBN denotes Truncated
T9618 12171-12174 NN denotes Apc
T9616 12175-12186 NNS denotes Transcripts
T9619 12186-12320 sentence denotes To assess the molecular effects of the K14-cre–mediated recombination, we screened for the presence of deleted Apc (ApcΔ580) alleles.
T9620 12187-12189 TO denotes To
T9621 12190-12196 VB denotes assess
T9623 12197-12200 DT denotes the
T9625 12201-12210 JJ denotes molecular
T9624 12211-12218 NNS denotes effects
T9626 12219-12221 IN denotes of
T9627 12222-12225 DT denotes the
T9629 12226-12229 NN denotes K14
T9631 12229-12230 HYPH denotes -
T9630 12230-12233 NN denotes cre
T9633 12233-12234 HYPH denotes –
T9632 12234-12242 JJ denotes mediated
T9628 12243-12256 NN denotes recombination
T9634 12256-12258 , denotes ,
T9635 12258-12260 PRP denotes we
T9622 12261-12269 VBD denotes screened
T9636 12270-12273 IN denotes for
T9637 12274-12277 DT denotes the
T9638 12278-12286 NN denotes presence
T9639 12287-12289 IN denotes of
T9640 12290-12297 VBN denotes deleted
T9641 12298-12301 NN denotes Apc
T9643 12302-12303 -LRB- denotes (
T9644 12303-12310 NN denotes ApcΔ580
T9645 12310-12311 -RRB- denotes )
T9642 12312-12319 NNS denotes alleles
T9646 12319-12320 . denotes .
T9647 12320-12472 sentence denotes Genomic DNA was extracted from liver, thymus, and skin from all 4 possible genotypes: K14-cre; ApcCKO/CKO, K14-cre; ApcCKO/+, ApcCKO/CKO, and ApcCKO/+.
T9648 12321-12328 JJ denotes Genomic
T9649 12329-12332 NN denotes DNA
T9651 12333-12336 VBD denotes was
T9650 12337-12346 VBN denotes extracted
T9652 12347-12351 IN denotes from
T9653 12352-12357 NN denotes liver
T9654 12357-12359 , denotes ,
T9655 12359-12365 NN denotes thymus
T9656 12365-12367 , denotes ,
T9657 12367-12370 CC denotes and
T9658 12371-12375 NN denotes skin
T9659 12376-12380 IN denotes from
T9660 12381-12384 DT denotes all
T9662 12385-12386 CD denotes 4
T9663 12387-12395 JJ denotes possible
T9661 12396-12405 NNS denotes genotypes
T9664 12405-12407 : denotes :
T9665 12407-12410 NN denotes K14
T9667 12410-12411 HYPH denotes -
T9666 12411-12414 NN denotes cre
T9668 12414-12415 : denotes ;
T9669 12416-12422 NN denotes ApcCKO
T9671 12422-12423 HYPH denotes /
T9670 12423-12426 NN denotes CKO
T9672 12426-12428 , denotes ,
T9673 12428-12431 NN denotes K14
T9675 12431-12432 HYPH denotes -
T9674 12432-12435 NN denotes cre
T9676 12435-12436 : denotes ;
T9677 12437-12443 NN denotes ApcCKO
T9678 12443-12444 HYPH denotes /
T9679 12444-12445 SYM denotes +
T9680 12445-12447 , denotes ,
T9681 12447-12453 NN denotes ApcCKO
T9683 12453-12454 HYPH denotes /
T9682 12454-12457 NN denotes CKO
T9684 12457-12459 , denotes ,
T9685 12459-12462 CC denotes and
T9686 12463-12469 NN denotes ApcCKO
T9687 12469-12470 HYPH denotes /
T9688 12470-12471 SYM denotes +
T9689 12471-12472 . denotes .
T9690 12472-12638 sentence denotes Genotyping on genomic DNA from these tissues showed that the ApcΔ580 allele (500-bp product) was detected only from the skin and thymus of the K14-cre–positive mice.
T9691 12473-12483 VBG denotes Genotyping
T9693 12484-12486 IN denotes on
T9694 12487-12494 JJ denotes genomic
T9695 12495-12498 NN denotes DNA
T9696 12499-12503 IN denotes from
T9697 12504-12509 DT denotes these
T9698 12510-12517 NNS denotes tissues
T9692 12518-12524 VBD denotes showed
T9699 12525-12529 IN denotes that
T9701 12530-12533 DT denotes the
T9703 12534-12541 NN denotes ApcΔ580
T9702 12542-12548 NN denotes allele
T9704 12549-12550 -LRB- denotes (
T9706 12550-12553 CD denotes 500
T9708 12553-12554 HYPH denotes -
T9707 12554-12556 NN denotes bp
T9705 12557-12564 NN denotes product
T9709 12564-12565 -RRB- denotes )
T9710 12566-12569 VBD denotes was
T9700 12570-12578 VBN denotes detected
T9711 12579-12583 RB denotes only
T9712 12584-12588 IN denotes from
T9713 12589-12592 DT denotes the
T9714 12593-12597 NN denotes skin
T9715 12598-12601 CC denotes and
T9716 12602-12608 NN denotes thymus
T9717 12609-12611 IN denotes of
T9718 12612-12615 DT denotes the
T9720 12616-12619 NN denotes K14
T9722 12619-12620 HYPH denotes -
T9721 12620-12623 NN denotes cre
T9724 12623-12624 HYPH denotes –
T9723 12624-12632 JJ denotes positive
T9719 12633-12637 NNS denotes mice
T9725 12637-12638 . denotes .
T9726 12638-12823 sentence denotes The presence of mutant Apc allele in the thymus of K14-cre; ApcCKO/+ mice was consistently much less than the DNA from the skin of the same animal or other tissues from the KA mutants.
T9727 12639-12642 DT denotes The
T9728 12643-12651 NN denotes presence
T9730 12652-12654 IN denotes of
T9731 12655-12661 JJ denotes mutant
T9733 12662-12665 NN denotes Apc
T9732 12666-12672 NN denotes allele
T9734 12673-12675 IN denotes in
T9735 12676-12679 DT denotes the
T9736 12680-12686 NN denotes thymus
T9737 12687-12689 IN denotes of
T9738 12690-12693 NN denotes K14
T9740 12693-12694 HYPH denotes -
T9739 12694-12697 NN denotes cre
T9742 12697-12698 : denotes ;
T9743 12699-12705 NN denotes ApcCKO
T9744 12705-12706 HYPH denotes /
T9745 12706-12707 SYM denotes +
T9741 12708-12712 NNS denotes mice
T9729 12713-12716 VBD denotes was
T9746 12717-12729 RB denotes consistently
T9747 12730-12734 RB denotes much
T9748 12735-12739 JJR denotes less
T9749 12740-12744 IN denotes than
T9750 12745-12748 DT denotes the
T9751 12749-12752 NN denotes DNA
T9752 12753-12757 IN denotes from
T9753 12758-12761 DT denotes the
T9754 12762-12766 NN denotes skin
T9755 12767-12769 IN denotes of
T9756 12770-12773 DT denotes the
T9758 12774-12778 JJ denotes same
T9757 12779-12785 NN denotes animal
T9759 12786-12788 CC denotes or
T9760 12789-12794 JJ denotes other
T9761 12795-12802 NNS denotes tissues
T9762 12803-12807 IN denotes from
T9763 12808-12811 DT denotes the
T9765 12812-12814 NN denotes KA
T9764 12815-12822 NNS denotes mutants
T9766 12822-12823 . denotes .
T9767 12823-13109 sentence denotes In addition, this product was not detected at all in either the liver of K14-cre–positive or in any of the K14-cre–negative mouse tissues samples, establishing that Cre-mediated recombination has taken place in the tissue-specific manner in the mice that inherited K14-cre (Figure 3A).
T9768 12824-12826 IN denotes In
T9770 12827-12835 NN denotes addition
T9771 12835-12837 , denotes ,
T9772 12837-12841 DT denotes this
T9773 12842-12849 NN denotes product
T9774 12850-12853 VBD denotes was
T9775 12854-12857 RB denotes not
T9769 12858-12866 VBN denotes detected
T9776 12867-12869 RB denotes at
T9777 12870-12873 RB denotes all
T9778 12874-12876 IN denotes in
T9779 12877-12883 CC denotes either
T9781 12884-12887 DT denotes the
T9780 12888-12893 NN denotes liver
T9782 12894-12896 IN denotes of
T9783 12897-12900 NN denotes K14
T9785 12900-12901 HYPH denotes -
T9784 12901-12904 NN denotes cre
T9787 12904-12905 HYPH denotes –
T9786 12905-12913 JJ denotes positive
T9788 12914-12916 CC denotes or
T9789 12917-12919 IN denotes in
T9790 12920-12923 DT denotes any
T9791 12924-12926 IN denotes of
T9792 12927-12930 DT denotes the
T9794 12931-12934 NN denotes K14
T9796 12934-12935 HYPH denotes -
T9795 12935-12938 NN denotes cre
T9797 12938-12939 HYPH denotes –
T9793 12939-12947 JJ denotes negative
T9798 12948-12953 NN denotes mouse
T9800 12954-12961 NNS denotes tissues
T9799 12962-12969 NNS denotes samples
T9801 12969-12971 , denotes ,
T9802 12971-12983 VBG denotes establishing
T9803 12984-12988 IN denotes that
T9805 12989-12992 NN denotes Cre
T9807 12992-12993 HYPH denotes -
T9806 12993-13001 JJ denotes mediated
T9808 13002-13015 NN denotes recombination
T9809 13016-13019 VBZ denotes has
T9804 13020-13025 VBN denotes taken
T9810 13026-13031 NN denotes place
T9811 13032-13034 IN denotes in
T9812 13035-13038 DT denotes the
T9814 13039-13045 NN denotes tissue
T9816 13045-13046 HYPH denotes -
T9815 13046-13054 JJ denotes specific
T9813 13055-13061 NN denotes manner
T9817 13062-13064 IN denotes in
T9818 13065-13068 DT denotes the
T9819 13069-13073 NNS denotes mice
T9820 13074-13078 WDT denotes that
T9821 13079-13088 VBD denotes inherited
T9822 13089-13092 NN denotes K14
T9824 13092-13093 HYPH denotes -
T9823 13093-13096 NN denotes cre
T9825 13097-13098 -LRB- denotes (
T9827 13098-13104 NN denotes Figure
T9826 13105-13107 NN denotes 3A
T9828 13107-13108 -RRB- denotes )
T9829 13108-13109 . denotes .
T9830 13109-13831 sentence denotes Figure 3 Tissue-Specific Detection and Expression of Deleted Apc Alleles (A) Tissue-specific genotyping PCR. Only genomic DNA samples from the skin (S) and thymus (T), but not liver (L) of mice positive for K14-cre show the presence of deleted ApcΔ580 allele. (B) Genotype- and tissue-specific expression of the truncated Apc transcripts. A representative gel of RT-PCR using primers F546 and R721, showing that only RNA from the skin and thymus but not liver of mice positive for K14-cre have transcripts from both wild-type (528 bp) and deleted (313 bp) Apc alleles. Apc transcripts were also analyzed by RT-PCR with primers spanning exon 14 (Figure 1A) using total RNA isolated from the corresponding tissue samples.
T9831 13681-13684 NN denotes Apc
T9832 13685-13696 NNS denotes transcripts
T9834 13697-13701 VBD denotes were
T9835 13702-13706 RB denotes also
T9833 13707-13715 VBN denotes analyzed
T9836 13716-13718 IN denotes by
T9837 13719-13721 NN denotes RT
T9839 13721-13722 HYPH denotes -
T9838 13722-13725 NN denotes PCR
T9840 13726-13730 IN denotes with
T9841 13731-13738 NNS denotes primers
T9842 13739-13747 VBG denotes spanning
T9843 13748-13752 NN denotes exon
T9844 13753-13755 CD denotes 14
T9845 13756-13757 -LRB- denotes (
T9847 13757-13763 NN denotes Figure
T9846 13764-13766 NN denotes 1A
T9848 13766-13767 -RRB- denotes )
T9849 13768-13773 VBG denotes using
T9850 13774-13779 JJ denotes total
T9851 13780-13783 NN denotes RNA
T9852 13784-13792 VBN denotes isolated
T9853 13793-13797 IN denotes from
T9854 13798-13801 DT denotes the
T9856 13802-13815 VBG denotes corresponding
T9857 13816-13822 NN denotes tissue
T9855 13823-13830 NNS denotes samples
T9858 13830-13831 . denotes .
T9859 13831-14017 sentence denotes We detected the expected RT-PCR product (313 bp) from the truncated Apc (ApcΔ580) allele only in the tissues where Cre recombinase is known to be expressed in the K14-cre–positive mice.
T9860 13832-13834 PRP denotes We
T9861 13835-13843 VBD denotes detected
T9862 13844-13847 DT denotes the
T9864 13848-13856 VBN denotes expected
T9865 13857-13859 NN denotes RT
T9867 13859-13860 HYPH denotes -
T9866 13860-13863 NN denotes PCR
T9863 13864-13871 NN denotes product
T9868 13872-13873 -LRB- denotes (
T9870 13873-13876 CD denotes 313
T9869 13877-13879 NN denotes bp
T9871 13879-13880 -RRB- denotes )
T9872 13881-13885 IN denotes from
T9873 13886-13889 DT denotes the
T9875 13890-13899 VBN denotes truncated
T9876 13900-13903 NN denotes Apc
T9877 13904-13905 -LRB- denotes (
T9878 13905-13912 NN denotes ApcΔ580
T9879 13912-13913 -RRB- denotes )
T9874 13914-13920 NN denotes allele
T9880 13921-13925 RB denotes only
T9881 13926-13928 IN denotes in
T9882 13929-13932 DT denotes the
T9883 13933-13940 NNS denotes tissues
T9884 13941-13946 WRB denotes where
T9886 13947-13950 NN denotes Cre
T9887 13951-13962 NN denotes recombinase
T9888 13963-13965 VBZ denotes is
T9885 13966-13971 VBN denotes known
T9889 13972-13974 TO denotes to
T9891 13975-13977 VB denotes be
T9890 13978-13987 VBN denotes expressed
T9892 13988-13990 IN denotes in
T9893 13991-13994 DT denotes the
T9895 13995-13998 NN denotes K14
T9897 13998-13999 HYPH denotes -
T9896 13999-14002 NN denotes cre
T9899 14002-14003 HYPH denotes –
T9898 14003-14011 JJ denotes positive
T9894 14012-14016 NNS denotes mice
T9900 14016-14017 . denotes .
T9901 14017-14365 sentence denotes However, this product was not detected in either the K14-cre–negative mouse tissues samples or the liver of K14-cre–positive mice, and only the product from the wild-type allele (528 bp) was detected from these RNA samples, further confirming that Cre-mediated recombination has taken place in the tissue- and genotype-specific manner (Figure 3B).
T9902 14018-14025 RB denotes However
T9904 14025-14027 , denotes ,
T9905 14027-14031 DT denotes this
T9906 14032-14039 NN denotes product
T9907 14040-14043 VBD denotes was
T9908 14044-14047 RB denotes not
T9903 14048-14056 VBN denotes detected
T9909 14057-14059 IN denotes in
T9910 14060-14066 CC denotes either
T9912 14067-14070 DT denotes the
T9913 14071-14074 NN denotes K14
T9915 14074-14075 HYPH denotes -
T9914 14075-14078 NN denotes cre
T9917 14078-14079 HYPH denotes –
T9916 14079-14087 JJ denotes negative
T9918 14088-14093 NN denotes mouse
T9919 14094-14101 NNS denotes tissues
T9911 14102-14109 NNS denotes samples
T9920 14110-14112 CC denotes or
T9921 14113-14116 DT denotes the
T9922 14117-14122 NN denotes liver
T9923 14123-14125 IN denotes of
T9924 14126-14129 NN denotes K14
T9926 14129-14130 HYPH denotes -
T9925 14130-14133 NN denotes cre
T9928 14133-14134 HYPH denotes –
T9927 14134-14142 JJ denotes positive
T9929 14143-14147 NNS denotes mice
T9930 14147-14149 , denotes ,
T9931 14149-14152 CC denotes and
T9932 14153-14157 RB denotes only
T9934 14158-14161 DT denotes the
T9933 14162-14169 NN denotes product
T9936 14170-14174 IN denotes from
T9937 14175-14178 DT denotes the
T9939 14179-14183 JJ denotes wild
T9941 14183-14184 HYPH denotes -
T9940 14184-14188 NN denotes type
T9938 14189-14195 NN denotes allele
T9942 14196-14197 -LRB- denotes (
T9944 14197-14200 CD denotes 528
T9943 14201-14203 NN denotes bp
T9945 14203-14204 -RRB- denotes )
T9946 14205-14208 VBD denotes was
T9935 14209-14217 VBN denotes detected
T9947 14218-14222 IN denotes from
T9948 14223-14228 DT denotes these
T9950 14229-14232 NN denotes RNA
T9949 14233-14240 NNS denotes samples
T9951 14240-14242 , denotes ,
T9952 14242-14249 RB denotes further
T9953 14250-14260 VBG denotes confirming
T9954 14261-14265 IN denotes that
T9956 14266-14269 NN denotes Cre
T9958 14269-14270 HYPH denotes -
T9957 14270-14278 JJ denotes mediated
T9959 14279-14292 NN denotes recombination
T9960 14293-14296 VBZ denotes has
T9955 14297-14302 VBN denotes taken
T9961 14303-14308 NN denotes place
T9962 14309-14311 IN denotes in
T9963 14312-14315 DT denotes the
T9965 14316-14322 NN denotes tissue
T9967 14322-14323 HYPH denotes -
T9968 14324-14327 CC denotes and
T9969 14328-14336 NN denotes genotype
T9970 14336-14337 HYPH denotes -
T9966 14337-14345 JJ denotes specific
T9964 14346-14352 NN denotes manner
T9971 14353-14354 -LRB- denotes (
T9973 14354-14360 NN denotes Figure
T9972 14361-14363 NN denotes 3B
T9974 14363-14364 -RRB- denotes )
T9975 14364-14365 . denotes .
T12568 14367-14370 JJ denotes K14
T12570 14370-14371 HYPH denotes -
T12569 14371-14374 JJ denotes cre
T12572 14374-14375 HYPH denotes –
T12571 14375-14381 JJ denotes Driven
T12574 14382-14385 NN denotes Apc
T12575 14386-14390 NN denotes Loss
T12576 14391-14398 VBN denotes Induced
T12577 14399-14407 NN denotes Aberrant
T12578 14408-14412 NN denotes Hair
T12573 14413-14422 NNS denotes Follicles
T12579 14423-14433 IN denotes throughout
T12580 14434-14437 DT denotes the
T12581 14438-14447 NN denotes Epidermis
T12582 14447-14608 sentence denotes To understand the basis for delayed and abnormal hair development in the KA mutants, we conducted a histological and immunohistochemical examination (Figure 4).
T12583 14448-14450 TO denotes To
T12584 14451-14461 VB denotes understand
T12586 14462-14465 DT denotes the
T12587 14466-14471 NN denotes basis
T12588 14472-14475 IN denotes for
T12589 14476-14483 VBN denotes delayed
T12591 14484-14487 CC denotes and
T12592 14488-14496 JJ denotes abnormal
T12593 14497-14501 NN denotes hair
T12590 14502-14513 NN denotes development
T12594 14514-14516 IN denotes in
T12595 14517-14520 DT denotes the
T12597 14521-14523 NN denotes KA
T12596 14524-14531 NNS denotes mutants
T12598 14531-14533 , denotes ,
T12599 14533-14535 PRP denotes we
T12585 14536-14545 VBD denotes conducted
T12600 14546-14547 DT denotes a
T12602 14548-14560 JJ denotes histological
T12603 14561-14564 CC denotes and
T12604 14565-14584 JJ denotes immunohistochemical
T12601 14585-14596 NN denotes examination
T12605 14597-14598 -LRB- denotes (
T12606 14598-14604 NN denotes Figure
T12607 14605-14606 CD denotes 4
T12608 14606-14607 -RRB- denotes )
T12609 14607-14608 . denotes .
T12610 14608-14757 sentence denotes The hair follicle is an epidermal appendage that consists of an upper permanent portion, and a lower cycling portion that produces the hair [22,23].
T12611 14609-14612 DT denotes The
T12613 14613-14617 NN denotes hair
T12612 14618-14626 NN denotes follicle
T12614 14627-14629 VBZ denotes is
T12615 14630-14632 DT denotes an
T12617 14633-14642 JJ denotes epidermal
T12616 14643-14652 NN denotes appendage
T12618 14653-14657 WDT denotes that
T12619 14658-14666 VBZ denotes consists
T12620 14667-14669 IN denotes of
T12621 14670-14672 DT denotes an
T12623 14673-14678 JJ denotes upper
T12624 14679-14688 JJ denotes permanent
T12622 14689-14696 NN denotes portion
T12625 14696-14698 , denotes ,
T12626 14698-14701 CC denotes and
T12627 14702-14703 DT denotes a
T12629 14704-14709 JJR denotes lower
T12630 14710-14717 NN denotes cycling
T12628 14718-14725 NN denotes portion
T12631 14726-14730 WDT denotes that
T12632 14731-14739 VBZ denotes produces
T12633 14740-14743 DT denotes the
T12634 14744-14748 NN denotes hair
T12635 14749-14750 -LRB- denotes [
T12637 14750-14752 CD denotes 22
T12638 14752-14753 , denotes ,
T12636 14753-14755 CD denotes 23
T12639 14755-14756 -RRB- denotes ]
T12640 14756-14757 . denotes .
T12641 14757-14867 sentence denotes The outer root sheath (ORS) is contiguous with and biochemically similar to the basal layer of the epidermis.
T12642 14758-14761 DT denotes The
T12644 14762-14767 JJ denotes outer
T12645 14768-14772 NN denotes root
T12643 14773-14779 NN denotes sheath
T12647 14780-14781 -LRB- denotes (
T12648 14781-14784 NN denotes ORS
T12649 14784-14785 -RRB- denotes )
T12646 14786-14788 VBZ denotes is
T12650 14789-14799 JJ denotes contiguous
T12651 14800-14804 IN denotes with
T12652 14805-14808 CC denotes and
T12653 14809-14822 RB denotes biochemically
T12654 14823-14830 JJ denotes similar
T12655 14831-14833 IN denotes to
T12656 14834-14837 DT denotes the
T12658 14838-14843 JJ denotes basal
T12657 14844-14849 NN denotes layer
T12659 14850-14852 IN denotes of
T12660 14853-14856 DT denotes the
T12661 14857-14866 NN denotes epidermis
T12662 14866-14867 . denotes .
T12663 14867-15011 sentence denotes The inner layers of the hair follicle include three concentric layers of inner root sheath and three concentric layers of hair-producing cells.
T12664 14868-14871 DT denotes The
T12666 14872-14877 JJ denotes inner
T12665 14878-14884 NNS denotes layers
T12668 14885-14887 IN denotes of
T12669 14888-14891 DT denotes the
T12671 14892-14896 NN denotes hair
T12670 14897-14905 NN denotes follicle
T12667 14906-14913 VBP denotes include
T12672 14914-14919 CD denotes three
T12674 14920-14930 JJ denotes concentric
T12673 14931-14937 NNS denotes layers
T12675 14938-14940 IN denotes of
T12676 14941-14946 JJ denotes inner
T12678 14947-14951 NN denotes root
T12677 14952-14958 NN denotes sheath
T12679 14959-14962 CC denotes and
T12680 14963-14968 CD denotes three
T12682 14969-14979 JJ denotes concentric
T12681 14980-14986 NNS denotes layers
T12683 14987-14989 IN denotes of
T12684 14990-14994 NN denotes hair
T12686 14994-14995 HYPH denotes -
T12685 14995-15004 VBG denotes producing
T12687 15005-15010 NNS denotes cells
T12688 15010-15011 . denotes .
T12689 15011-15241 sentence denotes At the base of the hair follicle is the germinative hair follicle bulb, which contains rapidly proliferating “matrix” cells that differentiate to populate all of the layers of the inner root sheath and the hair shaft itself [22].
T12690 15012-15014 IN denotes At
T12692 15015-15018 DT denotes the
T12693 15019-15023 NN denotes base
T12694 15024-15026 IN denotes of
T12695 15027-15030 DT denotes the
T12697 15031-15035 NN denotes hair
T12696 15036-15044 NN denotes follicle
T12691 15045-15047 VBZ denotes is
T12698 15048-15051 DT denotes the
T12700 15052-15063 JJ denotes germinative
T12701 15064-15068 NN denotes hair
T12702 15069-15077 NN denotes follicle
T12699 15078-15082 NN denotes bulb
T12703 15082-15084 , denotes ,
T12704 15084-15089 WDT denotes which
T12705 15090-15098 VBZ denotes contains
T12706 15099-15106 RB denotes rapidly
T12707 15107-15120 VBG denotes proliferating
T12709 15121-15122 `` denotes “
T12710 15122-15128 NN denotes matrix
T12711 15128-15129 '' denotes ”
T12708 15130-15135 NNS denotes cells
T12712 15136-15140 WDT denotes that
T12713 15141-15154 VBP denotes differentiate
T12714 15155-15157 TO denotes to
T12715 15158-15166 VB denotes populate
T12716 15167-15170 DT denotes all
T12717 15171-15173 IN denotes of
T12718 15174-15177 DT denotes the
T12719 15178-15184 NNS denotes layers
T12720 15185-15187 IN denotes of
T12721 15188-15191 DT denotes the
T12723 15192-15197 JJ denotes inner
T12724 15198-15202 NN denotes root
T12722 15203-15209 NN denotes sheath
T12725 15210-15213 CC denotes and
T12726 15214-15217 DT denotes the
T12728 15218-15222 NN denotes hair
T12727 15223-15228 NN denotes shaft
T12729 15229-15235 PRP denotes itself
T12730 15236-15237 -LRB- denotes [
T12731 15237-15239 CD denotes 22
T12732 15239-15240 -RRB- denotes ]
T12733 15240-15241 . denotes .
T12734 15241-15498 sentence denotes During the anagen phase of the hair cycle (until P15), hair follicles of phenotypically normal mice grew deeply into the subcutaneous fat and were uniformly spaced and aligned in parallel arrays at a specific angle relative to the skin surface (Figure 4A).
T12735 15242-15248 IN denotes During
T12737 15249-15252 DT denotes the
T12739 15253-15259 NN denotes anagen
T12738 15260-15265 NN denotes phase
T12740 15266-15268 IN denotes of
T12741 15269-15272 DT denotes the
T12743 15273-15277 NN denotes hair
T12742 15278-15283 NN denotes cycle
T12744 15284-15285 -LRB- denotes (
T12745 15285-15290 IN denotes until
T12746 15291-15294 NN denotes P15
T12747 15294-15295 -RRB- denotes )
T12748 15295-15297 , denotes ,
T12749 15297-15301 NN denotes hair
T12750 15302-15311 NNS denotes follicles
T12751 15312-15314 IN denotes of
T12752 15315-15329 RB denotes phenotypically
T12754 15330-15336 JJ denotes normal
T12753 15337-15341 NNS denotes mice
T12736 15342-15346 VBD denotes grew
T12755 15347-15353 RB denotes deeply
T12756 15354-15358 IN denotes into
T12757 15359-15362 DT denotes the
T12759 15363-15375 JJ denotes subcutaneous
T12758 15376-15379 NN denotes fat
T12760 15380-15383 CC denotes and
T12761 15384-15388 VBD denotes were
T12763 15389-15398 RB denotes uniformly
T12762 15399-15405 VBN denotes spaced
T12764 15406-15409 CC denotes and
T12765 15410-15417 VBN denotes aligned
T12766 15418-15420 IN denotes in
T12767 15421-15429 JJ denotes parallel
T12768 15430-15436 NNS denotes arrays
T12769 15437-15439 IN denotes at
T12770 15440-15441 DT denotes a
T12772 15442-15450 JJ denotes specific
T12771 15451-15456 NN denotes angle
T12773 15457-15465 JJ denotes relative
T12774 15466-15468 IN denotes to
T12775 15469-15472 DT denotes the
T12777 15473-15477 NN denotes skin
T12776 15478-15485 NN denotes surface
T12778 15486-15487 -LRB- denotes (
T12780 15487-15493 NN denotes Figure
T12779 15494-15496 NN denotes 4A
T12781 15496-15497 -RRB- denotes )
T12782 15497-15498 . denotes .
T12783 15498-15716 sentence denotes In contrast, KA mutant follicles were irregularly spaced and often seen as disoriented and clamped invaginations at P3 that became even more remarkable at P12 when the mutant mice were covered by fur coat (Figure 4F).
T12784 15499-15501 IN denotes In
T12786 15502-15510 NN denotes contrast
T12787 15510-15512 , denotes ,
T12788 15512-15514 NN denotes KA
T12790 15515-15521 JJ denotes mutant
T12789 15522-15531 NNS denotes follicles
T12791 15532-15536 VBD denotes were
T12792 15537-15548 RB denotes irregularly
T12785 15549-15555 VBN denotes spaced
T12793 15556-15559 CC denotes and
T12794 15560-15565 RB denotes often
T12795 15566-15570 VBN denotes seen
T12796 15571-15573 IN denotes as
T12797 15574-15585 JJ denotes disoriented
T12799 15586-15589 CC denotes and
T12800 15590-15597 JJ denotes clamped
T12798 15598-15611 NNS denotes invaginations
T12801 15612-15614 IN denotes at
T12802 15615-15617 NN denotes P3
T12803 15618-15622 WDT denotes that
T12804 15623-15629 VBD denotes became
T12805 15630-15634 RB denotes even
T12806 15635-15639 RBR denotes more
T12807 15640-15650 JJ denotes remarkable
T12808 15651-15653 IN denotes at
T12809 15654-15657 NN denotes P12
T12810 15658-15662 WRB denotes when
T12812 15663-15666 DT denotes the
T12814 15667-15673 JJ denotes mutant
T12813 15674-15678 NNS denotes mice
T12815 15679-15683 VBD denotes were
T12811 15684-15691 VBN denotes covered
T12816 15692-15694 IN denotes by
T12817 15695-15698 NN denotes fur
T12818 15699-15703 NN denotes coat
T12819 15704-15705 -LRB- denotes (
T12821 15705-15711 NN denotes Figure
T12820 15712-15714 NN denotes 4F
T12822 15714-15715 -RRB- denotes )
T12823 15715-15716 . denotes .
T12824 15716-15844 sentence denotes Bulbs were often bent in addition to being irregularly angled to one another and their sizes and locations were often variable.
T12825 15717-15722 NNS denotes Bulbs
T12826 15723-15727 VBD denotes were
T12827 15728-15733 RB denotes often
T12828 15734-15738 JJ denotes bent
T12829 15739-15741 IN denotes in
T12830 15742-15750 NN denotes addition
T12831 15751-15753 IN denotes to
T12832 15754-15759 VBG denotes being
T12833 15760-15771 RB denotes irregularly
T12834 15772-15778 JJ denotes angled
T12835 15779-15781 IN denotes to
T12836 15782-15785 NN denotes one
T12837 15786-15793 NN denotes another
T12838 15794-15797 CC denotes and
T12839 15798-15803 PRP$ denotes their
T12840 15804-15809 NNS denotes sizes
T12842 15810-15813 CC denotes and
T12843 15814-15823 NNS denotes locations
T12841 15824-15828 VBD denotes were
T12844 15829-15834 RB denotes often
T12845 15835-15843 JJ denotes variable
T12846 15843-15844 . denotes .
T12847 15844-16019 sentence denotes Clusters of multiple invaginations or dysplastic follicular structures were frequently observed throughout the epidermis, whereas other regions showed gaps with no follicles.
T12848 15845-15853 NNS denotes Clusters
T12850 15854-15856 IN denotes of
T12851 15857-15865 JJ denotes multiple
T12852 15866-15879 NNS denotes invaginations
T12853 15880-15882 CC denotes or
T12854 15883-15893 JJ denotes dysplastic
T12856 15894-15904 JJ denotes follicular
T12855 15905-15915 NNS denotes structures
T12857 15916-15920 VBD denotes were
T12858 15921-15931 RB denotes frequently
T12849 15932-15940 VBN denotes observed
T12859 15941-15951 IN denotes throughout
T12860 15952-15955 DT denotes the
T12861 15956-15965 NN denotes epidermis
T12862 15965-15967 , denotes ,
T12863 15967-15974 IN denotes whereas
T12865 15975-15980 JJ denotes other
T12866 15981-15988 NNS denotes regions
T12864 15989-15995 VBD denotes showed
T12867 15996-16000 NNS denotes gaps
T12868 16001-16005 IN denotes with
T12869 16006-16008 DT denotes no
T12870 16009-16018 NNS denotes follicles
T12871 16018-16019 . denotes .
T12872 16019-16151 sentence denotes Serial sectioning indicated that some of the hair follicles in the P12 mutant skin were not properly formed or shorter than normal.
T12873 16020-16026 JJ denotes Serial
T12874 16027-16037 NN denotes sectioning
T12875 16038-16047 VBD denotes indicated
T12876 16048-16052 IN denotes that
T12878 16053-16057 DT denotes some
T12879 16058-16060 IN denotes of
T12880 16061-16064 DT denotes the
T12882 16065-16069 NN denotes hair
T12881 16070-16079 NNS denotes follicles
T12883 16080-16082 IN denotes in
T12884 16083-16086 DT denotes the
T12886 16087-16090 NN denotes P12
T12887 16091-16097 JJ denotes mutant
T12885 16098-16102 NN denotes skin
T12877 16103-16107 VBD denotes were
T12888 16108-16111 RB denotes not
T12889 16112-16120 RB denotes properly
T12890 16121-16127 JJ denotes formed
T12891 16128-16130 CC denotes or
T12892 16131-16138 JJR denotes shorter
T12893 16139-16143 IN denotes than
T12894 16144-16150 JJ denotes normal
T12895 16150-16151 . denotes .
T12896 16151-16310 sentence denotes Taken together, these features could account for the apparently delayed, followed by outgrowth, of the short and shaggy-looking fur coat of these mutant mice.
T12897 16152-16157 VBN denotes Taken
T12899 16158-16166 RB denotes together
T12900 16166-16168 , denotes ,
T12901 16168-16173 DT denotes these
T12902 16174-16182 NNS denotes features
T12903 16183-16188 MD denotes could
T12898 16189-16196 VB denotes account
T12904 16197-16200 IN denotes for
T12905 16201-16204 DT denotes the
T12907 16205-16215 RB denotes apparently
T12906 16216-16223 VBN denotes delayed
T12908 16223-16225 , denotes ,
T12909 16225-16233 VBN denotes followed
T12910 16234-16236 IN denotes by
T12911 16237-16246 NN denotes outgrowth
T12912 16246-16248 , denotes ,
T12913 16248-16250 IN denotes of
T12914 16251-16254 DT denotes the
T12916 16255-16260 JJ denotes short
T12917 16261-16264 CC denotes and
T12918 16265-16271 JJ denotes shaggy
T12920 16271-16272 HYPH denotes -
T12919 16272-16279 JJ denotes looking
T12921 16280-16283 NN denotes fur
T12915 16284-16288 NN denotes coat
T12922 16289-16291 IN denotes of
T12923 16292-16297 DT denotes these
T12925 16298-16304 JJ denotes mutant
T12924 16305-16309 NNS denotes mice
T12926 16309-16310 . denotes .
T12927 16310-17283 sentence denotes Figure 4 Histological and Immunochemical Examination of P12 Skin and Teeth (A–E) P12 normal skin. (F–J) P12 mutant skin. (K–N) P12 normal oral cavity. (O–R) P12 mutant oral cavity. Stained with H&E for histology (A, F, K–L, O–P), Ki67 (B, G), β-catenin (C, H, M, Q), K14 (D, I, N, R), and K6 (E, J). Aberrant follicular morphogenesis, characterized by formation of irregularly spaced, nonpolarized hair follicles, in mutant skin is evident. Despite the abnormal histology, proliferation seems to be confined to hair bulb-like structures (arrows in [G], inset [G′] at higher magnification), but in mutant skin (arrows in [H], inset [H′] at higher magnification) and oral cavity (arrows in insets [Q′] at higher magnification) elevated cytosolic localization of β-catenin is detected in some cells. Scale bars: 50 μm for (A–F), (H–J); 250 μm for (K) and (O); 100 μm for (G), (L–N), (P–R); 20 μm for (Q′). Apc is a regulator of β-catenin that is important for Wnt signaling.
T12928 17215-17218 NN denotes Apc
T12929 17219-17221 VBZ denotes is
T12930 17222-17223 DT denotes a
T12931 17224-17233 NN denotes regulator
T12932 17234-17236 IN denotes of
T12933 17237-17238 NN denotes β
T12935 17238-17239 HYPH denotes -
T12934 17239-17246 NN denotes catenin
T12936 17247-17251 WDT denotes that
T12937 17252-17254 VBZ denotes is
T12938 17255-17264 JJ denotes important
T12939 17265-17268 IN denotes for
T12940 17269-17272 NN denotes Wnt
T12941 17273-17282 NN denotes signaling
T12942 17282-17283 . denotes .
T12943 17283-17360 sentence denotes We examined the patterns of expression of β-catenin in the affected tissues.
T12944 17284-17286 PRP denotes We
T12945 17287-17295 VBD denotes examined
T12946 17296-17299 DT denotes the
T12947 17300-17308 NNS denotes patterns
T12948 17309-17311 IN denotes of
T12949 17312-17322 NN denotes expression
T12950 17323-17325 IN denotes of
T12951 17326-17327 NN denotes β
T12953 17327-17328 HYPH denotes -
T12952 17328-17335 NN denotes catenin
T12954 17336-17338 IN denotes in
T12955 17339-17342 DT denotes the
T12957 17343-17351 JJ denotes affected
T12956 17352-17359 NNS denotes tissues
T12958 17359-17360 . denotes .
T12959 17360-17735 sentence denotes In the normal skin, β-catenin, a member of the adherens junction complex, was found in the ORS of hair follicles and basal layer of epidermis, where K14 expression is also observed (Figure 4C and 4D), whereas the expression of K1, involucrin, and loricrin (markers for spinous and granular layers of epidermis) was only observed in the nonbasal epidermis (unpublished data).
T12960 17361-17363 IN denotes In
T12962 17364-17367 DT denotes the
T12964 17368-17374 JJ denotes normal
T12963 17375-17379 NN denotes skin
T12965 17379-17381 , denotes ,
T12966 17381-17382 NN denotes β
T12968 17382-17383 HYPH denotes -
T12967 17383-17390 NN denotes catenin
T12969 17390-17392 , denotes ,
T12970 17392-17393 DT denotes a
T12971 17394-17400 NN denotes member
T12972 17401-17403 IN denotes of
T12973 17404-17407 DT denotes the
T12975 17408-17416 NNS denotes adherens
T12976 17417-17425 NN denotes junction
T12974 17426-17433 NN denotes complex
T12977 17433-17435 , denotes ,
T12978 17435-17438 VBD denotes was
T12961 17439-17444 VBN denotes found
T12979 17445-17447 IN denotes in
T12980 17448-17451 DT denotes the
T12981 17452-17455 NN denotes ORS
T12982 17456-17458 IN denotes of
T12983 17459-17463 NN denotes hair
T12984 17464-17473 NNS denotes follicles
T12985 17474-17477 CC denotes and
T12986 17478-17483 JJ denotes basal
T12987 17484-17489 NN denotes layer
T12988 17490-17492 IN denotes of
T12989 17493-17502 NN denotes epidermis
T12990 17502-17504 , denotes ,
T12991 17504-17509 WRB denotes where
T12993 17510-17513 NN denotes K14
T12994 17514-17524 NN denotes expression
T12995 17525-17527 VBZ denotes is
T12996 17528-17532 RB denotes also
T12992 17533-17541 VBN denotes observed
T12997 17542-17543 -LRB- denotes (
T12999 17543-17549 NN denotes Figure
T12998 17550-17552 NN denotes 4C
T13000 17553-17556 CC denotes and
T13001 17557-17559 NN denotes 4D
T13002 17559-17560 -RRB- denotes )
T13003 17560-17562 , denotes ,
T13004 17562-17569 IN denotes whereas
T13006 17570-17573 DT denotes the
T13007 17574-17584 NN denotes expression
T13008 17585-17587 IN denotes of
T13009 17588-17590 NN denotes K1
T13010 17590-17592 , denotes ,
T13011 17592-17602 NN denotes involucrin
T13012 17602-17604 , denotes ,
T13013 17604-17607 CC denotes and
T13014 17608-17616 NN denotes loricrin
T13015 17617-17618 -LRB- denotes (
T13016 17618-17625 NNS denotes markers
T13017 17626-17629 IN denotes for
T13018 17630-17637 JJ denotes spinous
T13020 17638-17641 CC denotes and
T13021 17642-17650 JJ denotes granular
T13019 17651-17657 NNS denotes layers
T13022 17658-17660 IN denotes of
T13023 17661-17670 NN denotes epidermis
T13024 17670-17671 -RRB- denotes )
T13025 17672-17675 VBD denotes was
T13026 17676-17680 RB denotes only
T13005 17681-17689 VBN denotes observed
T13027 17690-17692 IN denotes in
T13028 17693-17696 DT denotes the
T13030 17697-17705 JJ denotes nonbasal
T13029 17706-17715 NN denotes epidermis
T13031 17716-17717 -LRB- denotes (
T13033 17717-17728 JJ denotes unpublished
T13032 17729-17733 NNS denotes data
T13034 17733-17734 -RRB- denotes )
T13035 17734-17735 . denotes .
T13036 17735-17947 sentence denotes The patterns of expression of K14, K1, involucrin, and loricrin, in skin from mutant and normal littermate mice at P3–P17, showed no significant differences in the terminal differentiation (Figure 4A–4D, 4F–4I).
T13037 17736-17739 DT denotes The
T13038 17740-17748 NNS denotes patterns
T13040 17749-17751 IN denotes of
T13041 17752-17762 NN denotes expression
T13042 17763-17765 IN denotes of
T13043 17766-17769 NN denotes K14
T13044 17769-17771 , denotes ,
T13045 17771-17773 NN denotes K1
T13046 17773-17775 , denotes ,
T13047 17775-17785 NN denotes involucrin
T13048 17785-17787 , denotes ,
T13049 17787-17790 CC denotes and
T13050 17791-17799 NN denotes loricrin
T13051 17799-17801 , denotes ,
T13052 17801-17803 IN denotes in
T13053 17804-17808 NN denotes skin
T13054 17809-17813 IN denotes from
T13055 17814-17820 JJ denotes mutant
T13057 17821-17824 CC denotes and
T13058 17825-17831 JJ denotes normal
T13056 17832-17842 JJ denotes littermate
T13059 17843-17847 NNS denotes mice
T13060 17848-17850 IN denotes at
T13061 17851-17853 NN denotes P3
T13062 17853-17854 SYM denotes –
T13063 17854-17857 NN denotes P17
T13064 17857-17859 , denotes ,
T13039 17859-17865 VBD denotes showed
T13065 17866-17868 DT denotes no
T13067 17869-17880 JJ denotes significant
T13066 17881-17892 NNS denotes differences
T13068 17893-17895 IN denotes in
T13069 17896-17899 DT denotes the
T13071 17900-17908 JJ denotes terminal
T13070 17909-17924 NN denotes differentiation
T13072 17925-17926 -LRB- denotes (
T13074 17926-17932 NN denotes Figure
T13073 17933-17935 NN denotes 4A
T13075 17935-17936 SYM denotes –
T13076 17936-17938 NN denotes 4D
T13077 17938-17940 , denotes ,
T13078 17940-17942 NN denotes 4F
T13079 17942-17943 SYM denotes –
T13080 17943-17945 NN denotes 4I
T13081 17945-17946 -RRB- denotes )
T13082 17946-17947 . denotes .
T13083 17947-18143 sentence denotes Similarly, the pattern of expression of K6, which is normally only expressed in the suprabasal or inner layer of the ORS of the hair follicle but not in the epidermis (Figure 4E), did not change.
T13084 17948-17957 RB denotes Similarly
T13086 17957-17959 , denotes ,
T13087 17959-17962 DT denotes the
T13088 17963-17970 NN denotes pattern
T13089 17971-17973 IN denotes of
T13090 17974-17984 NN denotes expression
T13091 17985-17987 IN denotes of
T13092 17988-17990 NN denotes K6
T13093 17990-17992 , denotes ,
T13094 17992-17997 WDT denotes which
T13096 17998-18000 VBZ denotes is
T13097 18001-18009 RB denotes normally
T13098 18010-18014 RB denotes only
T13095 18015-18024 VBN denotes expressed
T13099 18025-18027 IN denotes in
T13100 18028-18031 DT denotes the
T13102 18032-18042 JJ denotes suprabasal
T13103 18043-18045 CC denotes or
T13104 18046-18051 JJ denotes inner
T13101 18052-18057 NN denotes layer
T13105 18058-18060 IN denotes of
T13106 18061-18064 DT denotes the
T13107 18065-18068 NN denotes ORS
T13108 18069-18071 IN denotes of
T13109 18072-18075 DT denotes the
T13111 18076-18080 NN denotes hair
T13110 18081-18089 NN denotes follicle
T13112 18090-18093 CC denotes but
T13113 18094-18097 RB denotes not
T13114 18098-18100 IN denotes in
T13115 18101-18104 DT denotes the
T13116 18105-18114 NN denotes epidermis
T13117 18115-18116 -LRB- denotes (
T13119 18116-18122 NN denotes Figure
T13118 18123-18125 NN denotes 4E
T13120 18125-18126 -RRB- denotes )
T13121 18126-18128 , denotes ,
T13122 18128-18131 VBD denotes did
T13123 18132-18135 RB denotes not
T13085 18136-18142 VB denotes change
T13124 18142-18143 . denotes .
T13125 18143-18290 sentence denotes Due to the abnormal and disorganized structure of hair follicles themselves, K6 localization highlighted the histological abnormality (Figure 4J).
T13126 18144-18147 IN denotes Due
T13128 18148-18150 IN denotes to
T13129 18151-18154 DT denotes the
T13131 18155-18163 JJ denotes abnormal
T13132 18164-18167 CC denotes and
T13133 18168-18180 JJ denotes disorganized
T13130 18181-18190 NN denotes structure
T13134 18191-18193 IN denotes of
T13135 18194-18198 NN denotes hair
T13136 18199-18208 NNS denotes follicles
T13137 18209-18219 PRP denotes themselves
T13138 18219-18221 , denotes ,
T13139 18221-18223 NN denotes K6
T13140 18224-18236 NN denotes localization
T13127 18237-18248 VBD denotes highlighted
T13141 18249-18252 DT denotes the
T13143 18253-18265 JJ denotes histological
T13142 18266-18277 NN denotes abnormality
T13144 18278-18279 -LRB- denotes (
T13146 18279-18285 NN denotes Figure
T13145 18286-18288 NN denotes 4J
T13147 18288-18289 -RRB- denotes )
T13148 18289-18290 . denotes .
T13149 18290-18459 sentence denotes Yet as in the normal skin, K6 was principally seen only in the suprabasal layer of the ORS that did not colocalize with the basal markers, K14 or K5 (Figure 4I and 4J).
T13150 18291-18294 RB denotes Yet
T13152 18295-18297 IN denotes as
T13153 18298-18300 IN denotes in
T13154 18301-18304 DT denotes the
T13156 18305-18311 JJ denotes normal
T13155 18312-18316 NN denotes skin
T13157 18316-18318 , denotes ,
T13158 18318-18320 NN denotes K6
T13159 18321-18324 VBD denotes was
T13160 18325-18336 RB denotes principally
T13151 18337-18341 VBN denotes seen
T13161 18342-18346 RB denotes only
T13162 18347-18349 IN denotes in
T13163 18350-18353 DT denotes the
T13165 18354-18364 JJ denotes suprabasal
T13164 18365-18370 NN denotes layer
T13166 18371-18373 IN denotes of
T13167 18374-18377 DT denotes the
T13168 18378-18381 NN denotes ORS
T13169 18382-18386 WDT denotes that
T13171 18387-18390 VBD denotes did
T13172 18391-18394 RB denotes not
T13170 18395-18405 VB denotes colocalize
T13173 18406-18410 IN denotes with
T13174 18411-18414 DT denotes the
T13176 18415-18420 JJ denotes basal
T13175 18421-18428 NNS denotes markers
T13177 18428-18430 , denotes ,
T13178 18430-18433 NN denotes K14
T13179 18434-18436 CC denotes or
T13180 18437-18439 NN denotes K5
T13181 18440-18441 -LRB- denotes (
T13183 18441-18447 NN denotes Figure
T13182 18448-18450 NN denotes 4I
T13184 18451-18454 CC denotes and
T13185 18455-18457 NN denotes 4J
T13186 18457-18458 -RRB- denotes )
T13187 18458-18459 . denotes .
T13188 18459-18611 sentence denotes In normal skin, proliferating cells were detected in either the basal layer of epidermis or in germinative hair follicle bulbs at the base (Figure 4B).
T13189 18460-18462 IN denotes In
T13191 18463-18469 JJ denotes normal
T13192 18470-18474 NN denotes skin
T13193 18474-18476 , denotes ,
T13194 18476-18489 VBG denotes proliferating
T13195 18490-18495 NNS denotes cells
T13196 18496-18500 VBD denotes were
T13190 18501-18509 VBN denotes detected
T13197 18510-18512 IN denotes in
T13198 18513-18519 CC denotes either
T13199 18520-18523 DT denotes the
T13201 18524-18529 JJ denotes basal
T13200 18530-18535 NN denotes layer
T13202 18536-18538 IN denotes of
T13203 18539-18548 NN denotes epidermis
T13204 18549-18551 CC denotes or
T13205 18552-18554 IN denotes in
T13206 18555-18566 JJ denotes germinative
T13208 18567-18571 NN denotes hair
T13209 18572-18580 NN denotes follicle
T13207 18581-18586 NNS denotes bulbs
T13210 18587-18589 IN denotes at
T13211 18590-18593 DT denotes the
T13212 18594-18598 NN denotes base
T13213 18599-18600 -LRB- denotes (
T13215 18600-18606 NN denotes Figure
T13214 18607-18609 NN denotes 4B
T13216 18609-18610 -RRB- denotes )
T13217 18610-18611 . denotes .
T13218 18611-18869 sentence denotes In the mutant skin, either BrdU incorporation or Ki67 expression was observed not only in cells in bulbs at the base of the hair follicle but also in bulb-like structures that were budding out from the ORS of the existing hair follicles (Figure 4G and 4G′).
T13219 18612-18614 IN denotes In
T13221 18615-18618 DT denotes the
T13223 18619-18625 JJ denotes mutant
T13222 18626-18630 NN denotes skin
T13224 18630-18632 , denotes ,
T13225 18632-18638 CC denotes either
T13227 18639-18643 NN denotes BrdU
T13226 18644-18657 NN denotes incorporation
T13228 18658-18660 CC denotes or
T13229 18661-18665 NN denotes Ki67
T13230 18666-18676 NN denotes expression
T13231 18677-18680 VBD denotes was
T13220 18681-18689 VBN denotes observed
T13232 18690-18693 RB denotes not
T13234 18694-18698 RB denotes only
T13233 18699-18701 IN denotes in
T13235 18702-18707 NNS denotes cells
T13236 18708-18710 IN denotes in
T13237 18711-18716 NNS denotes bulbs
T13238 18717-18719 IN denotes at
T13239 18720-18723 DT denotes the
T13240 18724-18728 NN denotes base
T13241 18729-18731 IN denotes of
T13242 18732-18735 DT denotes the
T13244 18736-18740 NN denotes hair
T13243 18741-18749 NN denotes follicle
T13245 18750-18753 CC denotes but
T13246 18754-18758 RB denotes also
T13247 18759-18761 IN denotes in
T13248 18762-18766 NN denotes bulb
T13250 18766-18767 HYPH denotes -
T13249 18767-18771 JJ denotes like
T13251 18772-18782 NNS denotes structures
T13252 18783-18787 WDT denotes that
T13254 18788-18792 VBD denotes were
T13253 18793-18800 VBG denotes budding
T13255 18801-18804 RP denotes out
T13256 18805-18809 IN denotes from
T13257 18810-18813 DT denotes the
T13258 18814-18817 NN denotes ORS
T13259 18818-18820 IN denotes of
T13260 18821-18824 DT denotes the
T13262 18825-18833 VBG denotes existing
T13263 18834-18838 NN denotes hair
T13261 18839-18848 NNS denotes follicles
T13264 18849-18850 -LRB- denotes (
T13266 18850-18856 NN denotes Figure
T13265 18857-18859 NN denotes 4G
T13267 18860-18863 CC denotes and
T13268 18864-18866 NN denotes 4G
T13269 18866-18867 SYM denotes ′
T13270 18867-18868 -RRB- denotes )
T13271 18868-18869 . denotes .
T13272 18869-18957 sentence denotes Each budding tip was becoming like a hair follicle bulb containing proliferating cells.
T13273 18870-18874 DT denotes Each
T13275 18875-18882 VBG denotes budding
T13274 18883-18886 NN denotes tip
T13277 18887-18890 VBD denotes was
T13276 18891-18899 VBG denotes becoming
T13278 18900-18904 IN denotes like
T13279 18905-18906 DT denotes a
T13281 18907-18911 NN denotes hair
T13282 18912-18920 NN denotes follicle
T13280 18921-18925 NN denotes bulb
T13283 18926-18936 VBG denotes containing
T13284 18937-18950 VBG denotes proliferating
T13285 18951-18956 NNS denotes cells
T13286 18956-18957 . denotes .
T13287 18957-19117 sentence denotes Hence, despite the abnormal histology in the mutant skin, proliferation seems to be confined to bulb-like structures as in the normal skin (Figure 4G and 4G′).
T13288 18958-18963 RB denotes Hence
T13290 18963-18965 , denotes ,
T13291 18965-18972 IN denotes despite
T13292 18973-18976 DT denotes the
T13294 18977-18985 JJ denotes abnormal
T13293 18986-18995 NN denotes histology
T13295 18996-18998 IN denotes in
T13296 18999-19002 DT denotes the
T13298 19003-19009 JJ denotes mutant
T13297 19010-19014 NN denotes skin
T13299 19014-19016 , denotes ,
T13300 19016-19029 NN denotes proliferation
T13289 19030-19035 VBZ denotes seems
T13301 19036-19038 TO denotes to
T13303 19039-19041 VB denotes be
T13302 19042-19050 VBN denotes confined
T13304 19051-19053 IN denotes to
T13305 19054-19058 NN denotes bulb
T13307 19058-19059 HYPH denotes -
T13306 19059-19063 JJ denotes like
T13308 19064-19074 NNS denotes structures
T13309 19075-19077 IN denotes as
T13310 19078-19080 IN denotes in
T13311 19081-19084 DT denotes the
T13313 19085-19091 JJ denotes normal
T13312 19092-19096 NN denotes skin
T13314 19097-19098 -LRB- denotes (
T13316 19098-19104 NN denotes Figure
T13315 19105-19107 NN denotes 4G
T13317 19108-19111 CC denotes and
T13318 19112-19114 NN denotes 4G
T13319 19114-19115 SYM denotes ′
T13320 19115-19116 -RRB- denotes )
T13321 19116-19117 . denotes .
T13322 19117-19251 sentence denotes The exact locations of hair follicle bulbs were not as easy to define for some mutant follicles due to their disorganized structures.
T13323 19118-19121 DT denotes The
T13325 19122-19127 JJ denotes exact
T13324 19128-19137 NNS denotes locations
T13327 19138-19140 IN denotes of
T13328 19141-19145 NN denotes hair
T13329 19146-19154 NN denotes follicle
T13330 19155-19160 NNS denotes bulbs
T13326 19161-19165 VBD denotes were
T13331 19166-19169 RB denotes not
T13332 19170-19172 RB denotes as
T13333 19173-19177 JJ denotes easy
T13334 19178-19180 TO denotes to
T13335 19181-19187 VB denotes define
T13336 19188-19191 IN denotes for
T13337 19192-19196 DT denotes some
T13339 19197-19203 JJ denotes mutant
T13338 19204-19213 NNS denotes follicles
T13340 19214-19217 IN denotes due
T13341 19218-19220 IN denotes to
T13342 19221-19226 PRP$ denotes their
T13344 19227-19239 JJ denotes disorganized
T13343 19240-19250 NNS denotes structures
T13345 19250-19251 . denotes .
T13346 19251-19462 sentence denotes Interestingly, in the mutant skin, in addition to diffuse membrane-bound localization as in the normal skin, cells with strong cytosolic β-catenin localization were also observed frequently (Figure 4H and 4H′).
T13347 19252-19265 RB denotes Interestingly
T13349 19265-19267 , denotes ,
T13350 19267-19269 IN denotes in
T13351 19270-19273 DT denotes the
T13353 19274-19280 JJ denotes mutant
T13352 19281-19285 NN denotes skin
T13354 19285-19287 , denotes ,
T13355 19287-19289 IN denotes in
T13356 19290-19298 NN denotes addition
T13357 19299-19301 IN denotes to
T13358 19302-19309 JJ denotes diffuse
T13360 19310-19318 NN denotes membrane
T13362 19318-19319 HYPH denotes -
T13361 19319-19324 JJ denotes bound
T13359 19325-19337 NN denotes localization
T13363 19338-19340 IN denotes as
T13364 19341-19343 IN denotes in
T13365 19344-19347 DT denotes the
T13367 19348-19354 JJ denotes normal
T13366 19355-19359 NN denotes skin
T13368 19359-19361 , denotes ,
T13369 19361-19366 NNS denotes cells
T13370 19367-19371 IN denotes with
T13371 19372-19378 JJ denotes strong
T13373 19379-19388 JJ denotes cytosolic
T13374 19389-19390 NN denotes β
T13375 19390-19391 HYPH denotes -
T13376 19391-19398 NN denotes catenin
T13372 19399-19411 NN denotes localization
T13377 19412-19416 VBD denotes were
T13378 19417-19421 RB denotes also
T13348 19422-19430 VBN denotes observed
T13379 19431-19441 RB denotes frequently
T13380 19442-19443 -LRB- denotes (
T13382 19443-19449 NN denotes Figure
T13381 19450-19452 NN denotes 4H
T13383 19453-19456 CC denotes and
T13384 19457-19459 NN denotes 4H
T13385 19459-19460 SYM denotes ′
T13386 19460-19461 -RRB- denotes )
T13387 19461-19462 . denotes .
T13388 19462-19582 sentence denotes These elevated β-catenin–expressing cells were usually surrounded by proliferating cells, forming bulb-like structures.
T13389 19463-19468 DT denotes These
T13391 19469-19477 JJ denotes elevated
T13392 19478-19479 NN denotes β
T13394 19479-19480 HYPH denotes -
T13393 19480-19487 NN denotes catenin
T13396 19487-19488 HYPH denotes –
T13395 19488-19498 VBG denotes expressing
T13390 19499-19504 NNS denotes cells
T13398 19505-19509 VBD denotes were
T13399 19510-19517 RB denotes usually
T13397 19518-19528 VBN denotes surrounded
T13400 19529-19531 IN denotes by
T13401 19532-19545 VBG denotes proliferating
T13402 19546-19551 NNS denotes cells
T13403 19551-19553 , denotes ,
T13404 19553-19560 VBG denotes forming
T13405 19561-19565 NN denotes bulb
T13407 19565-19566 HYPH denotes -
T13406 19566-19570 JJ denotes like
T13408 19571-19581 NNS denotes structures
T13409 19581-19582 . denotes .
T13410 19582-19851 sentence denotes Comparison of immunochemically stained serial sections showed that these intense cytosolic β-catenin stainings were usually found in either K14-positive K1-negative basal epidermis or K14-positive K6-negative basal ORS cells, and are surrounded by proliferating cells.
T13411 19583-19593 NN denotes Comparison
T13413 19594-19596 IN denotes of
T13414 19597-19613 RB denotes immunochemically
T13415 19614-19621 VBN denotes stained
T13417 19622-19628 JJ denotes serial
T13416 19629-19637 NNS denotes sections
T13412 19638-19644 VBD denotes showed
T13418 19645-19649 IN denotes that
T13420 19650-19655 DT denotes these
T13422 19656-19663 JJ denotes intense
T13423 19664-19673 JJ denotes cytosolic
T13424 19674-19675 NN denotes β
T13425 19675-19676 HYPH denotes -
T13426 19676-19683 NN denotes catenin
T13421 19684-19693 NNS denotes stainings
T13427 19694-19698 VBD denotes were
T13428 19699-19706 RB denotes usually
T13419 19707-19712 VBN denotes found
T13429 19713-19715 IN denotes in
T13430 19716-19722 CC denotes either
T13432 19723-19726 NN denotes K14
T13434 19726-19727 HYPH denotes -
T13433 19727-19735 JJ denotes positive
T13435 19736-19738 NN denotes K1
T13437 19738-19739 HYPH denotes -
T13436 19739-19747 JJ denotes negative
T13438 19748-19753 JJ denotes basal
T13431 19754-19763 NN denotes epidermis
T13439 19764-19766 CC denotes or
T13440 19767-19770 NN denotes K14
T13442 19770-19771 HYPH denotes -
T13441 19771-19779 JJ denotes positive
T13444 19780-19782 NN denotes K6
T13446 19782-19783 HYPH denotes -
T13445 19783-19791 JJ denotes negative
T13447 19792-19797 JJ denotes basal
T13448 19798-19801 NN denotes ORS
T13443 19802-19807 NNS denotes cells
T13449 19807-19809 , denotes ,
T13450 19809-19812 CC denotes and
T13451 19813-19816 VBP denotes are
T13452 19817-19827 VBN denotes surrounded
T13453 19828-19830 IN denotes by
T13454 19831-19844 VBG denotes proliferating
T13455 19845-19850 NNS denotes cells
T13456 19850-19851 . denotes .
T13457 19851-20051 sentence denotes To determine the initiation of hair follicle morphogenesis in these mutants, we examined the expression pattern of Sonic hedgehog (Shh), a factor expressed in hair bulbs in embryonic skin (Figure 5).
T13458 19852-19854 TO denotes To
T13459 19855-19864 VB denotes determine
T13461 19865-19868 DT denotes the
T13462 19869-19879 NN denotes initiation
T13463 19880-19882 IN denotes of
T13464 19883-19887 NN denotes hair
T13465 19888-19896 NN denotes follicle
T13466 19897-19910 NN denotes morphogenesis
T13467 19911-19913 IN denotes in
T13468 19914-19919 DT denotes these
T13469 19920-19927 NNS denotes mutants
T13470 19927-19929 , denotes ,
T13471 19929-19931 PRP denotes we
T13460 19932-19940 VBD denotes examined
T13472 19941-19944 DT denotes the
T13474 19945-19955 NN denotes expression
T13473 19956-19963 NN denotes pattern
T13475 19964-19966 IN denotes of
T13476 19967-19972 NNP denotes Sonic
T13477 19973-19981 NNP denotes hedgehog
T13478 19982-19983 -LRB- denotes (
T13479 19983-19986 NN denotes Shh
T13480 19986-19987 -RRB- denotes )
T13481 19987-19989 , denotes ,
T13482 19989-19990 DT denotes a
T13483 19991-19997 NN denotes factor
T13484 19998-20007 VBN denotes expressed
T13485 20008-20010 IN denotes in
T13486 20011-20015 NN denotes hair
T13487 20016-20021 NNS denotes bulbs
T13488 20022-20024 IN denotes in
T13489 20025-20034 JJ denotes embryonic
T13490 20035-20039 NN denotes skin
T13491 20040-20041 -LRB- denotes (
T13492 20041-20047 NN denotes Figure
T13493 20048-20049 CD denotes 5
T13494 20049-20050 -RRB- denotes )
T13495 20050-20051 . denotes .
T13496 20051-20311 sentence denotes The aberrant hair follicle morphogenesis is evident as early as E14.5 in mutant embryonic skin, by multiple apolarized expression of Shh throughout the epidermis (Figure 5B), whereas that of control embryos was well polarized and regularly spaced (Figure 5A).
T13497 20052-20055 DT denotes The
T13499 20056-20064 JJ denotes aberrant
T13500 20065-20069 NN denotes hair
T13501 20070-20078 NN denotes follicle
T13498 20079-20092 NN denotes morphogenesis
T13502 20093-20095 VBZ denotes is
T13503 20096-20103 JJ denotes evident
T13504 20104-20106 RB denotes as
T13505 20107-20112 RB denotes early
T13506 20113-20115 IN denotes as
T13507 20116-20121 NN denotes E14.5
T13508 20122-20124 IN denotes in
T13509 20125-20131 JJ denotes mutant
T13511 20132-20141 JJ denotes embryonic
T13510 20142-20146 NN denotes skin
T13512 20146-20148 , denotes ,
T13513 20148-20150 IN denotes by
T13514 20151-20159 JJ denotes multiple
T13516 20160-20170 JJ denotes apolarized
T13515 20171-20181 NN denotes expression
T13517 20182-20184 IN denotes of
T13518 20185-20188 NN denotes Shh
T13519 20189-20199 IN denotes throughout
T13520 20200-20203 DT denotes the
T13521 20204-20213 NN denotes epidermis
T13522 20214-20215 -LRB- denotes (
T13524 20215-20221 NN denotes Figure
T13523 20222-20224 NN denotes 5B
T13525 20224-20225 -RRB- denotes )
T13526 20225-20227 , denotes ,
T13527 20227-20234 IN denotes whereas
T13529 20235-20239 DT denotes that
T13530 20240-20242 IN denotes of
T13531 20243-20250 NN denotes control
T13532 20251-20258 NNS denotes embryos
T13528 20259-20262 VBD denotes was
T13533 20263-20267 RB denotes well
T13534 20268-20277 JJ denotes polarized
T13535 20278-20281 CC denotes and
T13536 20282-20291 RB denotes regularly
T13537 20292-20298 JJ denotes spaced
T13538 20299-20300 -LRB- denotes (
T13540 20300-20306 NN denotes Figure
T13539 20307-20309 NN denotes 5A
T13541 20309-20310 -RRB- denotes )
T13542 20310-20311 . denotes .
T13543 20311-20499 sentence denotes With development, control mouse hair follicles invaginate downward in a polarized manner (Figure 5C), whereas those of mutant embryos were completely irregular and apolarized (Figure 5D).
T13544 20312-20316 IN denotes With
T13546 20317-20328 NN denotes development
T13547 20328-20330 , denotes ,
T13548 20330-20337 NN denotes control
T13549 20338-20343 NN denotes mouse
T13551 20344-20348 NN denotes hair
T13550 20349-20358 NNS denotes follicles
T13545 20359-20369 VBP denotes invaginate
T13552 20370-20378 RB denotes downward
T13553 20379-20381 IN denotes in
T13554 20382-20383 DT denotes a
T13556 20384-20393 VBN denotes polarized
T13555 20394-20400 NN denotes manner
T13557 20401-20402 -LRB- denotes (
T13559 20402-20408 NN denotes Figure
T13558 20409-20411 NN denotes 5C
T13560 20411-20412 -RRB- denotes )
T13561 20412-20414 , denotes ,
T13562 20414-20421 IN denotes whereas
T13564 20422-20427 DT denotes those
T13565 20428-20430 IN denotes of
T13566 20431-20437 JJ denotes mutant
T13567 20438-20445 NNS denotes embryos
T13563 20446-20450 VBD denotes were
T13568 20451-20461 RB denotes completely
T13569 20462-20471 JJ denotes irregular
T13570 20472-20475 CC denotes and
T13571 20476-20486 JJ denotes apolarized
T13572 20487-20488 -LRB- denotes (
T13574 20488-20494 NN denotes Figure
T13573 20495-20497 NN denotes 5D
T13575 20497-20498 -RRB- denotes )
T13576 20498-20499 . denotes .
T13577 20499-20616 sentence denotes It was also noted that the size of each “budding” follicle, as detected by Shh expression, was variable (Figure 5D).
T13578 20500-20502 PRP denotes It
T13580 20503-20506 VBD denotes was
T13581 20507-20511 RB denotes also
T13579 20512-20517 VBN denotes noted
T13582 20518-20522 IN denotes that
T13584 20523-20526 DT denotes the
T13585 20527-20531 NN denotes size
T13586 20532-20534 IN denotes of
T13587 20535-20539 DT denotes each
T13589 20540-20541 `` denotes “
T13590 20541-20548 NN denotes budding
T13591 20548-20549 '' denotes ”
T13588 20550-20558 NN denotes follicle
T13592 20558-20560 , denotes ,
T13593 20560-20562 IN denotes as
T13594 20563-20571 VBN denotes detected
T13595 20572-20574 IN denotes by
T13596 20575-20578 NN denotes Shh
T13597 20579-20589 NN denotes expression
T13598 20589-20591 , denotes ,
T13583 20591-20594 VBD denotes was
T13599 20595-20603 JJ denotes variable
T13600 20604-20605 -LRB- denotes (
T13602 20605-20611 NN denotes Figure
T13601 20612-20614 NN denotes 5D
T13603 20614-20615 -RRB- denotes )
T13604 20615-20616 . denotes .
T13605 20616-20709 sentence denotes The intensity of Shh staining was generally stronger in mutant skin than in the normal skin.
T13606 20617-20620 DT denotes The
T13607 20621-20630 NN denotes intensity
T13609 20631-20633 IN denotes of
T13610 20634-20637 NN denotes Shh
T13611 20638-20646 NN denotes staining
T13608 20647-20650 VBD denotes was
T13612 20651-20660 RB denotes generally
T13613 20661-20669 JJR denotes stronger
T13614 20670-20672 IN denotes in
T13615 20673-20679 JJ denotes mutant
T13616 20680-20684 NN denotes skin
T13617 20685-20689 IN denotes than
T13618 20690-20692 IN denotes in
T13619 20693-20696 DT denotes the
T13621 20697-20703 JJ denotes normal
T13620 20704-20708 NN denotes skin
T13622 20708-20709 . denotes .
T13623 20709-20923 sentence denotes The aberrant initiation of multiple hair placodes during early hair follicle morphogenesis was also evident by the whole-mount in situ hybridization (ISH) of E15.5 mutant embryos for β-catenin (Figure 5F and 5F′).
T13624 20710-20713 DT denotes The
T13626 20714-20722 JJ denotes aberrant
T13625 20723-20733 NN denotes initiation
T13628 20734-20736 IN denotes of
T13629 20737-20745 JJ denotes multiple
T13631 20746-20750 NN denotes hair
T13630 20751-20759 NNS denotes placodes
T13632 20760-20766 IN denotes during
T13633 20767-20772 JJ denotes early
T13635 20773-20777 NN denotes hair
T13636 20778-20786 NN denotes follicle
T13634 20787-20800 NN denotes morphogenesis
T13627 20801-20804 VBD denotes was
T13637 20805-20809 RB denotes also
T13638 20810-20817 JJ denotes evident
T13639 20818-20820 IN denotes by
T13640 20821-20824 DT denotes the
T13642 20825-20830 JJ denotes whole
T13643 20830-20831 HYPH denotes -
T13641 20831-20836 NN denotes mount
T13644 20837-20839 FW denotes in
T13645 20840-20844 FW denotes situ
T13646 20845-20858 NN denotes hybridization
T13647 20859-20860 -LRB- denotes (
T13648 20860-20863 NN denotes ISH
T13649 20863-20864 -RRB- denotes )
T13650 20865-20867 IN denotes of
T13651 20868-20873 NN denotes E15.5
T13653 20874-20880 JJ denotes mutant
T13652 20881-20888 NNS denotes embryos
T13654 20889-20892 IN denotes for
T13655 20893-20894 NN denotes β
T13657 20894-20895 HYPH denotes -
T13656 20895-20902 NN denotes catenin
T13658 20903-20904 -LRB- denotes (
T13660 20904-20910 NN denotes Figure
T13659 20911-20913 NN denotes 5F
T13661 20914-20917 CC denotes and
T13662 20918-20920 NN denotes 5F
T13663 20920-20921 SYM denotes ′
T13664 20921-20922 -RRB- denotes )
T13665 20922-20923 . denotes .
T13666 20923-21233 sentence denotes The expression pattern of β-catenin in embryos clearly demonstrated the formation of regular arrays of hair placodes in the normal embryonic skin (Figure 5E and 5E′), but such regular patterning was lost, and often tightly clustered abnormal hair placodes were initiated in mutant embryonic skin (Figure 5F′).
T13667 20924-20927 DT denotes The
T13669 20928-20938 NN denotes expression
T13668 20939-20946 NN denotes pattern
T13671 20947-20949 IN denotes of
T13672 20950-20951 NN denotes β
T13674 20951-20952 HYPH denotes -
T13673 20952-20959 NN denotes catenin
T13675 20960-20962 IN denotes in
T13676 20963-20970 NNS denotes embryos
T13677 20971-20978 RB denotes clearly
T13670 20979-20991 VBD denotes demonstrated
T13678 20992-20995 DT denotes the
T13679 20996-21005 NN denotes formation
T13680 21006-21008 IN denotes of
T13681 21009-21016 JJ denotes regular
T13682 21017-21023 NNS denotes arrays
T13683 21024-21026 IN denotes of
T13684 21027-21031 NN denotes hair
T13685 21032-21040 NNS denotes placodes
T13686 21041-21043 IN denotes in
T13687 21044-21047 DT denotes the
T13689 21048-21054 JJ denotes normal
T13690 21055-21064 JJ denotes embryonic
T13688 21065-21069 NN denotes skin
T13691 21070-21071 -LRB- denotes (
T13693 21071-21077 NN denotes Figure
T13692 21078-21080 NN denotes 5E
T13694 21081-21084 CC denotes and
T13695 21085-21087 NN denotes 5E
T13696 21087-21088 SYM denotes ′
T13697 21088-21089 -RRB- denotes )
T13698 21089-21091 , denotes ,
T13699 21091-21094 CC denotes but
T13700 21095-21099 JJ denotes such
T13702 21100-21107 JJ denotes regular
T13701 21108-21118 NN denotes patterning
T13704 21119-21122 VBD denotes was
T13703 21123-21127 VBN denotes lost
T13705 21127-21129 , denotes ,
T13706 21129-21132 CC denotes and
T13707 21133-21138 RB denotes often
T13709 21139-21146 RB denotes tightly
T13710 21147-21156 VBN denotes clustered
T13712 21157-21165 JJ denotes abnormal
T13713 21166-21170 NN denotes hair
T13711 21171-21179 NNS denotes placodes
T13714 21180-21184 VBD denotes were
T13708 21185-21194 VBN denotes initiated
T13715 21195-21197 IN denotes in
T13716 21198-21204 JJ denotes mutant
T13717 21205-21214 JJ denotes embryonic
T13718 21215-21219 NN denotes skin
T13719 21220-21221 -LRB- denotes (
T13721 21221-21227 NN denotes Figure
T13720 21228-21230 NN denotes 5F
T13722 21230-21231 SYM denotes ′
T13723 21231-21232 -RRB- denotes )
T13724 21232-21233 . denotes .
T13725 21233-21410 sentence denotes Aberrant hair placodes were also evident throughout the skin surface of limbs in E15.5 mutants (Figure 5F), whereas those of the control embryos had not yet formed (Figure 5E).
T13726 21234-21242 JJ denotes Aberrant
T13728 21243-21247 NN denotes hair
T13727 21248-21256 NNS denotes placodes
T13729 21257-21261 VBD denotes were
T13730 21262-21266 RB denotes also
T13731 21267-21274 JJ denotes evident
T13732 21275-21285 IN denotes throughout
T13733 21286-21289 DT denotes the
T13735 21290-21294 NN denotes skin
T13734 21295-21302 NN denotes surface
T13736 21303-21305 IN denotes of
T13737 21306-21311 NNS denotes limbs
T13738 21312-21314 IN denotes in
T13739 21315-21320 NN denotes E15.5
T13740 21321-21328 NNS denotes mutants
T13741 21329-21330 -LRB- denotes (
T13743 21330-21336 NN denotes Figure
T13742 21337-21339 NN denotes 5F
T13744 21339-21340 -RRB- denotes )
T13745 21340-21342 , denotes ,
T13746 21342-21349 IN denotes whereas
T13748 21350-21355 DT denotes those
T13749 21356-21358 IN denotes of
T13750 21359-21362 DT denotes the
T13752 21363-21370 NN denotes control
T13751 21371-21378 NNS denotes embryos
T13753 21379-21382 VBD denotes had
T13754 21383-21386 RB denotes not
T13755 21387-21390 RB denotes yet
T13747 21391-21397 VBN denotes formed
T13756 21398-21399 -LRB- denotes (
T13758 21399-21405 NN denotes Figure
T13757 21406-21408 NN denotes 5E
T13759 21408-21409 -RRB- denotes )
T13760 21409-21410 . denotes .
T13761 21410-21694 sentence denotes Most interestingly, in the mutant footpads, where hair placodes do not normally form (Figure 5G), we also found ectopic irregularly sized and spaced hair placodes, indicating that the footpads still have the potential to form hair placodes in the absence of the Apc gene (Figure 5H).
T13762 21411-21415 RBS denotes Most
T13763 21416-21429 RB denotes interestingly
T13765 21429-21431 , denotes ,
T13766 21431-21433 IN denotes in
T13767 21434-21437 DT denotes the
T13769 21438-21444 JJ denotes mutant
T13768 21445-21453 NNS denotes footpads
T13770 21453-21455 , denotes ,
T13771 21455-21460 WRB denotes where
T13773 21461-21465 NN denotes hair
T13774 21466-21474 NNS denotes placodes
T13775 21475-21477 VBP denotes do
T13776 21478-21481 RB denotes not
T13777 21482-21490 RB denotes normally
T13772 21491-21495 VB denotes form
T13778 21496-21497 -LRB- denotes (
T13780 21497-21503 NN denotes Figure
T13779 21504-21506 NN denotes 5G
T13781 21506-21507 -RRB- denotes )
T13782 21507-21509 , denotes ,
T13783 21509-21511 PRP denotes we
T13784 21512-21516 RB denotes also
T13764 21517-21522 VBD denotes found
T13785 21523-21530 JJ denotes ectopic
T13787 21531-21542 RB denotes irregularly
T13788 21543-21548 VBN denotes sized
T13789 21549-21552 CC denotes and
T13790 21553-21559 VBN denotes spaced
T13791 21560-21564 NN denotes hair
T13786 21565-21573 NNS denotes placodes
T13792 21573-21575 , denotes ,
T13793 21575-21585 VBG denotes indicating
T13794 21586-21590 IN denotes that
T13796 21591-21594 DT denotes the
T13797 21595-21603 NNS denotes footpads
T13798 21604-21609 RB denotes still
T13795 21610-21614 VBP denotes have
T13799 21615-21618 DT denotes the
T13800 21619-21628 NN denotes potential
T13801 21629-21631 TO denotes to
T13802 21632-21636 VB denotes form
T13803 21637-21641 NN denotes hair
T13804 21642-21650 NNS denotes placodes
T13805 21651-21653 IN denotes in
T13806 21654-21657 DT denotes the
T13807 21658-21665 NN denotes absence
T13808 21666-21668 IN denotes of
T13809 21669-21672 DT denotes the
T13811 21673-21676 NN denotes Apc
T13810 21677-21681 NN denotes gene
T13812 21682-21683 -LRB- denotes (
T13814 21683-21689 NN denotes Figure
T13813 21690-21692 NN denotes 5H
T13815 21692-21693 -RRB- denotes )
T13816 21693-21694 . denotes .
T13817 21694-22641 sentence denotes Figure 5 Expression of Shh and β-catenin Transcripts in Normal (ApcCKO/CKO) and Mutant (K14-cre; ApcCKO/CKO) Embryonic Skin (A–D) Section ISH with Shh probe in E14.5 normal (A), E14.5 mutant (B), E16.5 normal (C), and E16.5 mutant (D) skin. Broken lines indicate the interface between epithelium and mesenchyme. Scale bars: 50 μm. Whole mount in situ detection of β-catenin in E15.5 normal (E, G), mutant (F, H) embryos. Aberrant initiation of multiple hair placodes is evident at E14.5. Loss of K14-driven Apc loss caused aberrant pattern formation (F′) and formed ectopic hair placodes in normally hairless foot pads (H, arrows) which are absent in normal (G). These results collectively suggest that the terminal differentiation does take place normally in the mutant skin, but initiation of embryonic hair follicle morphogenesis is severely disrupted, accompanied by a continuous ectopic hair follicle morphogenesis in postnatal mutant skin.
T13818 22359-22364 DT denotes These
T13819 22365-22372 NNS denotes results
T13821 22373-22385 RB denotes collectively
T13820 22386-22393 VBP denotes suggest
T13822 22394-22398 IN denotes that
T13824 22399-22402 DT denotes the
T13826 22403-22411 JJ denotes terminal
T13825 22412-22427 NN denotes differentiation
T13827 22428-22432 VBZ denotes does
T13823 22433-22437 VB denotes take
T13828 22438-22443 NN denotes place
T13829 22444-22452 RB denotes normally
T13830 22453-22455 IN denotes in
T13831 22456-22459 DT denotes the
T13833 22460-22466 JJ denotes mutant
T13832 22467-22471 NN denotes skin
T13834 22471-22473 , denotes ,
T13835 22473-22476 CC denotes but
T13836 22477-22487 NN denotes initiation
T13838 22488-22490 IN denotes of
T13839 22491-22500 JJ denotes embryonic
T13841 22501-22505 NN denotes hair
T13842 22506-22514 NN denotes follicle
T13840 22515-22528 NN denotes morphogenesis
T13843 22529-22531 VBZ denotes is
T13844 22532-22540 RB denotes severely
T13845 22541-22550 VBN denotes disrupted
T13846 22550-22552 , denotes ,
T13837 22552-22563 VBN denotes accompanied
T13847 22564-22566 IN denotes by
T13848 22567-22568 DT denotes a
T13850 22569-22579 JJ denotes continuous
T13851 22580-22587 JJ denotes ectopic
T13852 22588-22592 NN denotes hair
T13853 22593-22601 NN denotes follicle
T13849 22602-22615 NN denotes morphogenesis
T13854 22616-22618 IN denotes in
T13855 22619-22628 JJ denotes postnatal
T13857 22629-22635 JJ denotes mutant
T13856 22636-22640 NN denotes skin
T13858 22640-22641 . denotes .
T14505 22643-22650 NNS denotes Effects
T14506 22651-22653 IN denotes of
T14507 22654-22657 NN denotes K14
T14509 22657-22658 HYPH denotes -
T14508 22658-22661 NN denotes cre
T14511 22661-22662 HYPH denotes –
T14510 22662-22668 VBN denotes Driven
T14512 22669-22673 NN denotes Loss
T14513 22674-22676 IN denotes of
T14514 22677-22680 NN denotes Apc
T14515 22681-22683 IN denotes in
T14516 22684-22689 JJ denotes Other
T14518 22690-22699 JJ denotes Epidermal
T14517 22700-22710 NNS denotes Appendages
T14519 22710-22911 sentence denotes Similar to the biology of hair follicles, K14-cre–driven loss of Apc also affected the development of other epidermal appendages that depend on epithelial–mesenchymal interactions for their formation.
T14520 22711-22718 JJ denotes Similar
T14522 22719-22721 IN denotes to
T14523 22722-22725 DT denotes the
T14524 22726-22733 NN denotes biology
T14525 22734-22736 IN denotes of
T14526 22737-22741 NN denotes hair
T14527 22742-22751 NNS denotes follicles
T14528 22751-22753 , denotes ,
T14529 22753-22756 NN denotes K14
T14531 22756-22757 HYPH denotes -
T14530 22757-22760 NN denotes cre
T14533 22760-22761 HYPH denotes –
T14532 22761-22767 VBN denotes driven
T14534 22768-22772 NN denotes loss
T14535 22773-22775 IN denotes of
T14536 22776-22779 NN denotes Apc
T14537 22780-22784 RB denotes also
T14521 22785-22793 VBD denotes affected
T14538 22794-22797 DT denotes the
T14539 22798-22809 NN denotes development
T14540 22810-22812 IN denotes of
T14541 22813-22818 JJ denotes other
T14543 22819-22828 JJ denotes epidermal
T14542 22829-22839 NNS denotes appendages
T14544 22840-22844 WDT denotes that
T14545 22845-22851 VBP denotes depend
T14546 22852-22854 IN denotes on
T14547 22855-22865 JJ denotes epithelial
T14549 22865-22866 HYPH denotes –
T14548 22866-22877 JJ denotes mesenchymal
T14550 22878-22890 NNS denotes interactions
T14551 22891-22894 IN denotes for
T14552 22895-22900 PRP$ denotes their
T14553 22901-22910 NN denotes formation
T14554 22910-22911 . denotes .
T14555 22911-22975 sentence denotes The most striking of these was dental dysplasia (Figure 4K–4R).
T14556 22912-22915 DT denotes The
T14558 22916-22920 RBS denotes most
T14557 22921-22929 JJ denotes striking
T14560 22930-22932 IN denotes of
T14561 22933-22938 DT denotes these
T14559 22939-22942 VBD denotes was
T14562 22943-22949 JJ denotes dental
T14563 22950-22959 NN denotes dysplasia
T14564 22960-22961 -LRB- denotes (
T14566 22961-22967 NN denotes Figure
T14565 22968-22970 NN denotes 4K
T14567 22970-22971 SYM denotes –
T14568 22971-22973 NN denotes 4R
T14569 22973-22974 -RRB- denotes )
T14570 22974-22975 . denotes .
T14571 22975-23177 sentence denotes Tooth development is normally initiated between E11 and E12 by invagination of ectodermally derived oral epithelium into the underlying cranial neural crest–derived mesenchyme, generating a tooth germ.
T14572 22976-22981 NN denotes Tooth
T14573 22982-22993 NN denotes development
T14575 22994-22996 VBZ denotes is
T14576 22997-23005 RB denotes normally
T14574 23006-23015 VBN denotes initiated
T14577 23016-23023 IN denotes between
T14578 23024-23027 NN denotes E11
T14579 23028-23031 CC denotes and
T14580 23032-23035 NN denotes E12
T14581 23036-23038 IN denotes by
T14582 23039-23051 NN denotes invagination
T14583 23052-23054 IN denotes of
T14584 23055-23067 RB denotes ectodermally
T14585 23068-23075 VBN denotes derived
T14587 23076-23080 JJ denotes oral
T14586 23081-23091 NN denotes epithelium
T14588 23092-23096 IN denotes into
T14589 23097-23100 DT denotes the
T14591 23101-23111 VBG denotes underlying
T14592 23112-23119 JJ denotes cranial
T14594 23120-23126 JJ denotes neural
T14593 23127-23132 NN denotes crest
T14596 23132-23133 HYPH denotes –
T14595 23133-23140 VBN denotes derived
T14590 23141-23151 NN denotes mesenchyme
T14597 23151-23153 , denotes ,
T14598 23153-23163 VBG denotes generating
T14599 23164-23165 DT denotes a
T14601 23166-23171 JJ denotes tooth
T14600 23172-23176 NN denotes germ
T14602 23176-23177 . denotes .
T14603 23177-23341 sentence denotes Despite the grossly toothless phenotype of KA mutants, histological analysis of their oral cavities revealed the formation of multiple tooth buds at each location.
T14604 23178-23185 IN denotes Despite
T14606 23186-23189 DT denotes the
T14608 23190-23197 RB denotes grossly
T14609 23198-23207 JJ denotes toothless
T14607 23208-23217 NN denotes phenotype
T14610 23218-23220 IN denotes of
T14611 23221-23223 NN denotes KA
T14612 23224-23231 NNS denotes mutants
T14613 23231-23233 , denotes ,
T14614 23233-23245 JJ denotes histological
T14615 23246-23254 NN denotes analysis
T14616 23255-23257 IN denotes of
T14617 23258-23263 PRP$ denotes their
T14619 23264-23268 JJ denotes oral
T14618 23269-23277 NNS denotes cavities
T14605 23278-23286 VBD denotes revealed
T14620 23287-23290 DT denotes the
T14621 23291-23300 NN denotes formation
T14622 23301-23303 IN denotes of
T14623 23304-23312 JJ denotes multiple
T14625 23313-23318 JJ denotes tooth
T14624 23319-23323 NNS denotes buds
T14626 23324-23326 IN denotes at
T14627 23327-23331 DT denotes each
T14628 23332-23340 NN denotes location
T14629 23340-23341 . denotes .
T14630 23341-23446 sentence denotes These aberrant teeth obviously failed to grow out during the dietary transition from milk to solid food.
T14631 23342-23347 DT denotes These
T14633 23348-23356 JJ denotes aberrant
T14632 23357-23362 NNS denotes teeth
T14635 23363-23372 RB denotes obviously
T14634 23373-23379 VBD denotes failed
T14636 23380-23382 TO denotes to
T14637 23383-23387 VB denotes grow
T14638 23388-23391 RP denotes out
T14639 23392-23398 IN denotes during
T14640 23399-23402 DT denotes the
T14642 23403-23410 JJ denotes dietary
T14641 23411-23421 NN denotes transition
T14643 23422-23426 IN denotes from
T14644 23427-23431 NN denotes milk
T14645 23432-23434 IN denotes to
T14646 23435-23440 JJ denotes solid
T14647 23441-23445 NN denotes food
T14648 23445-23446 . denotes .
T14649 23446-23650 sentence denotes Analogous to the expression patterns of K14 and β-catenin in the normal skin, diffuse membrane-bound expression of β-catenin was detected in K14-expressing oral epithelium and ameloblasts of normal mice.
T14650 23447-23456 JJ denotes Analogous
T14652 23457-23459 IN denotes to
T14653 23460-23463 DT denotes the
T14655 23464-23474 NN denotes expression
T14654 23475-23483 NNS denotes patterns
T14656 23484-23486 IN denotes of
T14657 23487-23490 NN denotes K14
T14658 23491-23494 CC denotes and
T14659 23495-23496 NN denotes β
T14661 23496-23497 HYPH denotes -
T14660 23497-23504 NN denotes catenin
T14662 23505-23507 IN denotes in
T14663 23508-23511 DT denotes the
T14665 23512-23518 JJ denotes normal
T14664 23519-23523 NN denotes skin
T14666 23523-23525 , denotes ,
T14667 23525-23532 JJ denotes diffuse
T14669 23533-23541 NN denotes membrane
T14671 23541-23542 HYPH denotes -
T14670 23542-23547 VBN denotes bound
T14668 23548-23558 NN denotes expression
T14672 23559-23561 IN denotes of
T14673 23562-23563 NN denotes β
T14675 23563-23564 HYPH denotes -
T14674 23564-23571 NN denotes catenin
T14676 23572-23575 VBD denotes was
T14651 23576-23584 VBN denotes detected
T14677 23585-23587 IN denotes in
T14678 23588-23591 NN denotes K14
T14680 23591-23592 HYPH denotes -
T14679 23592-23602 VBG denotes expressing
T14682 23603-23607 JJ denotes oral
T14681 23608-23618 NN denotes epithelium
T14683 23619-23622 CC denotes and
T14684 23623-23634 NNS denotes ameloblasts
T14685 23635-23637 IN denotes of
T14686 23638-23644 JJ denotes normal
T14687 23645-23649 NNS denotes mice
T14688 23649-23650 . denotes .
T14689 23650-23809 sentence denotes In mutants, some of the K14-expressing cells also showed strong cytosolic/nuclear β-catenin staining, as observed in the mutant skin (Figure 4Q, 4Q′, and 4R).
T14690 23651-23653 IN denotes In
T14692 23654-23661 NNS denotes mutants
T14693 23661-23663 , denotes ,
T14694 23663-23667 DT denotes some
T14695 23668-23670 IN denotes of
T14696 23671-23674 DT denotes the
T14698 23675-23678 NN denotes K14
T14700 23678-23679 HYPH denotes -
T14699 23679-23689 VBG denotes expressing
T14697 23690-23695 NNS denotes cells
T14701 23696-23700 RB denotes also
T14691 23701-23707 VBD denotes showed
T14702 23708-23714 JJ denotes strong
T14704 23715-23724 JJ denotes cytosolic
T14706 23724-23725 HYPH denotes /
T14707 23725-23732 JJ denotes nuclear
T14705 23733-23734 NN denotes β
T14708 23734-23735 HYPH denotes -
T14709 23735-23742 NN denotes catenin
T14703 23743-23751 NN denotes staining
T14710 23751-23753 , denotes ,
T14711 23753-23755 IN denotes as
T14712 23756-23764 VBN denotes observed
T14713 23765-23767 IN denotes in
T14714 23768-23771 DT denotes the
T14716 23772-23778 JJ denotes mutant
T14715 23779-23783 NN denotes skin
T14717 23784-23785 -LRB- denotes (
T14719 23785-23791 NN denotes Figure
T14718 23792-23794 NN denotes 4Q
T14720 23794-23796 , denotes ,
T14721 23796-23798 NN denotes 4Q
T14722 23798-23799 SYM denotes ′
T14723 23799-23801 , denotes ,
T14724 23801-23804 CC denotes and
T14725 23805-23807 NN denotes 4R
T14726 23807-23808 -RRB- denotes )
T14727 23808-23809 . denotes .
T14728 23809-23962 sentence denotes Initiation of ectopic tooth buds in the mutant mice was evident at E15.5 by extra dots of Shh expression adjacent to the primary teeth (data not shown).
T14729 23810-23820 NN denotes Initiation
T14731 23821-23823 IN denotes of
T14732 23824-23831 JJ denotes ectopic
T14734 23832-23837 JJ denotes tooth
T14733 23838-23842 NNS denotes buds
T14735 23843-23845 IN denotes in
T14736 23846-23849 DT denotes the
T14738 23850-23856 JJ denotes mutant
T14737 23857-23861 NNS denotes mice
T14730 23862-23865 VBD denotes was
T14739 23866-23873 JJ denotes evident
T14740 23874-23876 IN denotes at
T14741 23877-23882 NN denotes E15.5
T14742 23883-23885 IN denotes by
T14743 23886-23891 JJ denotes extra
T14744 23892-23896 NNS denotes dots
T14745 23897-23899 IN denotes of
T14746 23900-23903 NN denotes Shh
T14747 23904-23914 NN denotes expression
T14748 23915-23923 JJ denotes adjacent
T14749 23924-23926 IN denotes to
T14750 23927-23930 DT denotes the
T14752 23931-23938 JJ denotes primary
T14751 23939-23944 NNS denotes teeth
T14753 23945-23946 -LRB- denotes (
T14755 23946-23950 NNS denotes data
T14756 23951-23954 RB denotes not
T14754 23955-23960 VBN denotes shown
T14757 23960-23961 -RRB- denotes )
T14758 23961-23962 . denotes .
T14759 23962-24090 sentence denotes Loss of Apc also leads to hyperplasia in squamous epithelia of cornea, oral, salivary, and Hardarian glands (unpublished data).
T14760 23963-23967 NN denotes Loss
T14762 23968-23970 IN denotes of
T14763 23971-23974 NN denotes Apc
T14764 23975-23979 RB denotes also
T14761 23980-23985 VBZ denotes leads
T14765 23986-23988 IN denotes to
T14766 23989-24000 NN denotes hyperplasia
T14767 24001-24003 IN denotes in
T14768 24004-24012 JJ denotes squamous
T14769 24013-24022 NNS denotes epithelia
T14770 24023-24025 IN denotes of
T14771 24026-24032 NN denotes cornea
T14773 24032-24034 , denotes ,
T14774 24034-24038 JJ denotes oral
T14775 24038-24040 , denotes ,
T14776 24040-24048 JJ denotes salivary
T14777 24048-24050 , denotes ,
T14778 24050-24053 CC denotes and
T14779 24054-24063 JJ denotes Hardarian
T14772 24064-24070 NNS denotes glands
T14780 24071-24072 -LRB- denotes (
T14782 24072-24083 JJ denotes unpublished
T14781 24084-24088 NNS denotes data
T14783 24088-24089 -RRB- denotes )
T14784 24089-24090 . denotes .
T14785 24090-24218 sentence denotes Squamous metaplasia to hair follicle–like structures, ectopic hair follicle morphogenesis was also observed in these epithelia.
T14786 24091-24099 JJ denotes Squamous
T14787 24100-24110 NN denotes metaplasia
T14789 24111-24113 IN denotes to
T14790 24114-24118 NN denotes hair
T14791 24119-24127 NN denotes follicle
T14793 24127-24128 HYPH denotes –
T14792 24128-24132 JJ denotes like
T14794 24133-24143 NNS denotes structures
T14795 24143-24145 , denotes ,
T14796 24145-24152 JJ denotes ectopic
T14798 24153-24157 NN denotes hair
T14799 24158-24166 NN denotes follicle
T14797 24167-24180 NN denotes morphogenesis
T14800 24181-24184 VBD denotes was
T14801 24185-24189 RB denotes also
T14788 24190-24198 VBN denotes observed
T14802 24199-24201 IN denotes in
T14803 24202-24207 DT denotes these
T14804 24208-24217 NNS denotes epithelia
T14805 24217-24218 . denotes .
T17241 24220-24223 NN denotes K14
T17243 24223-24224 HYPH denotes -
T17242 24224-24227 NN denotes cre
T17245 24227-24228 HYPH denotes –
T17244 24228-24234 VBN denotes Driven
T17247 24235-24238 NN denotes Apc
T17246 24239-24243 NN denotes Loss
T17248 24244-24251 VBZ denotes Results
T17249 24252-24254 IN denotes in
T17250 24255-24266 JJ denotes Hypoplastic
T17252 24266-24267 HYPH denotes /
T17251 24267-24274 JJ denotes Athymic
T17253 24275-24279 NNS denotes Mice
T17254 24279-24346 sentence denotes Thymus is an organ that is also known to have K14 expression [16].
T17255 24280-24286 NN denotes Thymus
T17256 24287-24289 VBZ denotes is
T17257 24290-24292 DT denotes an
T17258 24293-24298 NN denotes organ
T17259 24299-24303 WDT denotes that
T17261 24304-24306 VBZ denotes is
T17262 24307-24311 RB denotes also
T17260 24312-24317 VBN denotes known
T17263 24318-24320 TO denotes to
T17264 24321-24325 VB denotes have
T17265 24326-24329 NN denotes K14
T17266 24330-24340 NN denotes expression
T17267 24341-24342 -LRB- denotes [
T17268 24342-24344 CD denotes 16
T17269 24344-24345 -RRB- denotes ]
T17270 24345-24346 . denotes .
T17271 24346-24428 sentence denotes It represents the primary lymphoid organ for thymocyte development and selection.
T17272 24347-24349 PRP denotes It
T17273 24350-24360 VBZ denotes represents
T17274 24361-24364 DT denotes the
T17276 24365-24372 JJ denotes primary
T17277 24373-24381 JJ denotes lymphoid
T17275 24382-24387 NN denotes organ
T17278 24388-24391 IN denotes for
T17279 24392-24401 NN denotes thymocyte
T17280 24402-24413 NN denotes development
T17281 24414-24417 CC denotes and
T17282 24418-24427 NN denotes selection
T17283 24427-24428 . denotes .
T17284 24428-24521 sentence denotes Distinct population of TECs of cortex and medulla mediates both of these critical functions.
T17285 24429-24437 JJ denotes Distinct
T17286 24438-24448 NN denotes population
T17288 24449-24451 IN denotes of
T17289 24452-24456 NNS denotes TECs
T17290 24457-24459 IN denotes of
T17291 24460-24466 NN denotes cortex
T17292 24467-24470 CC denotes and
T17293 24471-24478 NN denotes medulla
T17287 24479-24487 VBZ denotes mediates
T17294 24488-24492 DT denotes both
T17295 24493-24495 IN denotes of
T17296 24496-24501 DT denotes these
T17298 24502-24510 JJ denotes critical
T17297 24511-24520 NNS denotes functions
T17299 24520-24521 . denotes .
T17300 24521-24648 sentence denotes Cortical and medullary TEC subsets are characterized by differential expression of four keratin species: K8, K18, K5, and K14.
T17301 24522-24530 JJ denotes Cortical
T17303 24531-24534 CC denotes and
T17304 24535-24544 JJ denotes medullary
T17305 24545-24548 NN denotes TEC
T17302 24549-24556 NNS denotes subsets
T17307 24557-24560 VBP denotes are
T17306 24561-24574 VBN denotes characterized
T17308 24575-24577 IN denotes by
T17309 24578-24590 JJ denotes differential
T17310 24591-24601 NN denotes expression
T17311 24602-24604 IN denotes of
T17312 24605-24609 CD denotes four
T17314 24610-24617 NN denotes keratin
T17313 24618-24625 NNS denotes species
T17315 24625-24627 : denotes :
T17316 24627-24629 NN denotes K8
T17317 24629-24631 , denotes ,
T17318 24631-24634 NN denotes K18
T17319 24634-24636 , denotes ,
T17320 24636-24638 NN denotes K5
T17321 24638-24640 , denotes ,
T17322 24640-24643 CC denotes and
T17323 24644-24647 NN denotes K14
T17324 24647-24648 . denotes .
T17325 24648-24809 sentence denotes The normal thymus is a lobulated lymphoid organ, each lobule clearly showing the two distinct TEC compartments, an outer cortex and an inner medulla (Figure 6).
T17326 24649-24652 DT denotes The
T17328 24653-24659 JJ denotes normal
T17327 24660-24666 NN denotes thymus
T17329 24667-24669 VBZ denotes is
T17330 24670-24671 DT denotes a
T17332 24672-24681 VBN denotes lobulated
T17333 24682-24690 JJ denotes lymphoid
T17331 24691-24696 NN denotes organ
T17334 24696-24698 , denotes ,
T17335 24698-24702 DT denotes each
T17336 24703-24709 NN denotes lobule
T17338 24710-24717 RB denotes clearly
T17337 24718-24725 VBG denotes showing
T17339 24726-24729 DT denotes the
T17341 24730-24733 CD denotes two
T17342 24734-24742 JJ denotes distinct
T17343 24743-24746 NN denotes TEC
T17340 24747-24759 NNS denotes compartments
T17344 24759-24761 , denotes ,
T17345 24761-24763 DT denotes an
T17347 24764-24769 JJ denotes outer
T17346 24770-24776 NN denotes cortex
T17348 24777-24780 CC denotes and
T17349 24781-24783 DT denotes an
T17351 24784-24789 JJ denotes inner
T17350 24790-24797 NN denotes medulla
T17352 24798-24799 -LRB- denotes (
T17353 24799-24805 NN denotes Figure
T17354 24806-24807 CD denotes 6
T17355 24807-24808 -RRB- denotes )
T17356 24808-24809 . denotes .
T17357 24809-24933 sentence denotes There were no major differences in the histology of thymus between the ages P3 to P17 in phenotypically normal littermates.
T17358 24810-24815 EX denotes There
T17359 24816-24820 VBD denotes were
T17360 24821-24823 DT denotes no
T17362 24824-24829 JJ denotes major
T17361 24830-24841 NNS denotes differences
T17363 24842-24844 IN denotes in
T17364 24845-24848 DT denotes the
T17365 24849-24858 NN denotes histology
T17366 24859-24861 IN denotes of
T17367 24862-24868 NN denotes thymus
T17368 24869-24876 IN denotes between
T17369 24877-24880 DT denotes the
T17370 24881-24885 NNS denotes ages
T17371 24886-24888 NN denotes P3
T17372 24889-24891 IN denotes to
T17373 24892-24895 NN denotes P17
T17374 24896-24898 IN denotes in
T17375 24899-24913 RB denotes phenotypically
T17376 24914-24920 JJ denotes normal
T17377 24921-24932 NNS denotes littermates
T17378 24932-24933 . denotes .
T17379 24933-25052 sentence denotes As shown in the H&E staining of thymus, the cortex was formed of dense lymphoid tissue that lacks nodules (Figure 6A).
T17380 24934-24936 IN denotes As
T17381 24937-24942 VBN denotes shown
T17383 24943-24945 IN denotes in
T17384 24946-24949 DT denotes the
T17386 24950-24951 NN denotes H
T17387 24951-24952 CC denotes &
T17388 24952-24953 NN denotes E
T17385 24954-24962 NN denotes staining
T17389 24963-24965 IN denotes of
T17390 24966-24972 NN denotes thymus
T17391 24972-24974 , denotes ,
T17392 24974-24977 DT denotes the
T17393 24978-24984 NN denotes cortex
T17394 24985-24988 VBD denotes was
T17382 24989-24995 VBN denotes formed
T17395 24996-24998 IN denotes of
T17396 24999-25004 JJ denotes dense
T17398 25005-25013 JJ denotes lymphoid
T17397 25014-25020 NN denotes tissue
T17399 25021-25025 WDT denotes that
T17400 25026-25031 VBZ denotes lacks
T17401 25032-25039 NNS denotes nodules
T17402 25040-25041 -LRB- denotes (
T17404 25041-25047 NN denotes Figure
T17403 25048-25050 NN denotes 6A
T17405 25050-25051 -RRB- denotes )
T17406 25051-25052 . denotes .
T17407 25052-25196 sentence denotes Since the stroma of the medulla is less heavily infiltrated with lymphocytes than the cortex, the medulla stained more lightly than the cortex.
T17408 25053-25058 IN denotes Since
T17410 25059-25062 DT denotes the
T17411 25063-25069 NN denotes stroma
T17412 25070-25072 IN denotes of
T17413 25073-25076 DT denotes the
T17414 25077-25084 NN denotes medulla
T17409 25085-25087 VBZ denotes is
T17416 25088-25092 RBR denotes less
T17417 25093-25100 RB denotes heavily
T17418 25101-25112 JJ denotes infiltrated
T17419 25113-25117 IN denotes with
T17420 25118-25129 NNS denotes lymphocytes
T17421 25130-25134 IN denotes than
T17422 25135-25138 DT denotes the
T17423 25139-25145 NN denotes cortex
T17424 25145-25147 , denotes ,
T17425 25147-25150 DT denotes the
T17426 25151-25158 NN denotes medulla
T17415 25159-25166 VBD denotes stained
T17427 25167-25171 RBR denotes more
T17428 25172-25179 RB denotes lightly
T17429 25180-25184 IN denotes than
T17430 25185-25188 DT denotes the
T17431 25189-25195 NN denotes cortex
T17432 25195-25196 . denotes .
T17433 25196-25303 sentence denotes In normal mice, the thymus retains its size until the young adult age and regresses thereafter by atrophy.
T17434 25197-25199 IN denotes In
T17436 25200-25206 JJ denotes normal
T17437 25207-25211 NNS denotes mice
T17438 25211-25213 , denotes ,
T17439 25213-25216 DT denotes the
T17440 25217-25223 NN denotes thymus
T17435 25224-25231 VBZ denotes retains
T17441 25232-25235 PRP$ denotes its
T17442 25236-25240 NN denotes size
T17443 25241-25246 IN denotes until
T17444 25247-25250 DT denotes the
T17446 25251-25256 JJ denotes young
T17447 25257-25262 JJ denotes adult
T17445 25263-25266 NN denotes age
T17448 25267-25270 CC denotes and
T17449 25271-25280 VBZ denotes regresses
T17450 25281-25291 RB denotes thereafter
T17451 25292-25294 IN denotes by
T17452 25295-25302 NN denotes atrophy
T17453 25302-25303 . denotes .
T17454 25303-25468 sentence denotes In the normal young mice we examined (P3–P17), it is evident that thymocytes were mitotically active in the cortex as determined by BrdU immunostaining (Figure 6B).
T17455 25304-25306 IN denotes In
T17457 25307-25310 DT denotes the
T17459 25311-25317 JJ denotes normal
T17460 25318-25323 JJ denotes young
T17458 25324-25328 NNS denotes mice
T17461 25329-25331 PRP denotes we
T17462 25332-25340 VBD denotes examined
T17463 25341-25342 -LRB- denotes (
T17464 25342-25344 NN denotes P3
T17465 25344-25345 SYM denotes –
T17466 25345-25348 NN denotes P17
T17467 25348-25349 -RRB- denotes )
T17468 25349-25351 , denotes ,
T17469 25351-25353 PRP denotes it
T17456 25354-25356 VBZ denotes is
T17470 25357-25364 JJ denotes evident
T17471 25365-25369 IN denotes that
T17473 25370-25380 NNS denotes thymocytes
T17472 25381-25385 VBD denotes were
T17474 25386-25397 RB denotes mitotically
T17475 25398-25404 JJ denotes active
T17476 25405-25407 IN denotes in
T17477 25408-25411 DT denotes the
T17478 25412-25418 NN denotes cortex
T17479 25419-25421 IN denotes as
T17480 25422-25432 VBN denotes determined
T17481 25433-25435 IN denotes by
T17482 25436-25440 NN denotes BrdU
T17483 25441-25455 NN denotes immunostaining
T17484 25456-25457 -LRB- denotes (
T17486 25457-25463 NN denotes Figure
T17485 25464-25466 NN denotes 6B
T17487 25466-25467 -RRB- denotes )
T17488 25467-25468 . denotes .
T17489 25468-25728 sentence denotes Immunohistochemistry of normal thymus from P3 to P17 mice showed a similar staining pattern for K14 in that its expression was restricted to a small population of TECs in the inner medullary region and in the keratinocytes in Hassall's corpuscles (Figure 6D).
T17490 25469-25489 NN denotes Immunohistochemistry
T17492 25490-25492 IN denotes of
T17493 25493-25499 JJ denotes normal
T17494 25500-25506 NN denotes thymus
T17495 25507-25511 IN denotes from
T17496 25512-25514 NN denotes P3
T17498 25515-25517 IN denotes to
T17499 25518-25521 NN denotes P17
T17497 25522-25526 NNS denotes mice
T17491 25527-25533 VBD denotes showed
T17500 25534-25535 DT denotes a
T17502 25536-25543 JJ denotes similar
T17503 25544-25552 NN denotes staining
T17501 25553-25560 NN denotes pattern
T17504 25561-25564 IN denotes for
T17505 25565-25568 NN denotes K14
T17506 25569-25571 IN denotes in
T17508 25572-25576 IN denotes that
T17509 25577-25580 PRP$ denotes its
T17510 25581-25591 NN denotes expression
T17511 25592-25595 VBD denotes was
T17507 25596-25606 VBN denotes restricted
T17512 25607-25609 IN denotes to
T17513 25610-25611 DT denotes a
T17515 25612-25617 JJ denotes small
T17514 25618-25628 NN denotes population
T17516 25629-25631 IN denotes of
T17517 25632-25636 NNS denotes TECs
T17518 25637-25639 IN denotes in
T17519 25640-25643 DT denotes the
T17521 25644-25649 JJ denotes inner
T17522 25650-25659 JJ denotes medullary
T17520 25660-25666 NN denotes region
T17523 25667-25670 CC denotes and
T17524 25671-25673 IN denotes in
T17525 25674-25677 DT denotes the
T17526 25678-25691 NNS denotes keratinocytes
T17527 25692-25694 IN denotes in
T17528 25695-25702 NNP denotes Hassall
T17530 25702-25704 POS denotes 's
T17529 25705-25715 NNS denotes corpuscles
T17531 25716-25717 -LRB- denotes (
T17533 25717-25723 NN denotes Figure
T17532 25724-25726 NN denotes 6D
T17534 25726-25727 -RRB- denotes )
T17535 25727-25728 . denotes .
T17536 25728-25836 sentence denotes Diffuse cytoplasmic staining for β-catenin was also detected in the medullary epithelial cells (Figure 6C).
T17537 25729-25736 JJ denotes Diffuse
T17539 25737-25748 JJ denotes cytoplasmic
T17538 25749-25757 NN denotes staining
T17541 25758-25761 IN denotes for
T17542 25762-25763 NN denotes β
T17544 25763-25764 HYPH denotes -
T17543 25764-25771 NN denotes catenin
T17545 25772-25775 VBD denotes was
T17546 25776-25780 RB denotes also
T17540 25781-25789 VBN denotes detected
T17547 25790-25792 IN denotes in
T17548 25793-25796 DT denotes the
T17550 25797-25806 JJ denotes medullary
T17551 25807-25817 JJ denotes epithelial
T17549 25818-25823 NNS denotes cells
T17552 25824-25825 -LRB- denotes (
T17554 25825-25831 NN denotes Figure
T17553 25832-25834 NN denotes 6C
T17555 25834-25835 -RRB- denotes )
T17556 25835-25836 . denotes .
T17557 25836-25968 sentence denotes In contrast to K14 expression, diffuse staining for K8 was observed in epithelial cells both in the medulla and cortex (Figure S3).
T17558 25837-25839 IN denotes In
T17560 25840-25848 NN denotes contrast
T17561 25849-25851 IN denotes to
T17562 25852-25855 NN denotes K14
T17563 25856-25866 NN denotes expression
T17564 25866-25868 , denotes ,
T17565 25868-25875 JJ denotes diffuse
T17566 25876-25884 NN denotes staining
T17567 25885-25888 IN denotes for
T17568 25889-25891 NN denotes K8
T17569 25892-25895 VBD denotes was
T17559 25896-25904 VBN denotes observed
T17570 25905-25907 IN denotes in
T17571 25908-25918 JJ denotes epithelial
T17572 25919-25924 NNS denotes cells
T17573 25925-25929 CC denotes both
T17574 25930-25932 IN denotes in
T17575 25933-25936 DT denotes the
T17576 25937-25944 NN denotes medulla
T17577 25945-25948 CC denotes and
T17578 25949-25955 NN denotes cortex
T17579 25956-25957 -LRB- denotes (
T17581 25957-25963 NN denotes Figure
T17580 25964-25966 NN denotes S3
T17582 25966-25967 -RRB- denotes )
T17583 25967-25968 . denotes .
T17584 25968-26128 sentence denotes K1 staining was not detected in young mice at P3 but in older mice it was detected in differentiated keratinocytes in some of Hassall's corpuscles (Figure S3).
T17585 25969-25971 NN denotes K1
T17586 25972-25980 NN denotes staining
T17588 25981-25984 VBD denotes was
T17589 25985-25988 RB denotes not
T17587 25989-25997 VBN denotes detected
T17590 25998-26000 IN denotes in
T17591 26001-26006 JJ denotes young
T17592 26007-26011 NNS denotes mice
T17593 26012-26014 IN denotes at
T17594 26015-26017 NN denotes P3
T17595 26018-26021 CC denotes but
T17596 26022-26024 IN denotes in
T17598 26025-26030 JJR denotes older
T17599 26031-26035 NNS denotes mice
T17600 26036-26038 PRP denotes it
T17601 26039-26042 VBD denotes was
T17597 26043-26051 VBN denotes detected
T17602 26052-26054 IN denotes in
T17603 26055-26069 VBN denotes differentiated
T17604 26070-26083 NNS denotes keratinocytes
T17605 26084-26086 IN denotes in
T17606 26087-26091 DT denotes some
T17607 26092-26094 IN denotes of
T17608 26095-26102 NNP denotes Hassall
T17610 26102-26104 POS denotes 's
T17609 26105-26115 NNS denotes corpuscles
T17611 26116-26117 -LRB- denotes (
T17613 26117-26123 NN denotes Figure
T17612 26124-26126 NN denotes S3
T17614 26126-26127 -RRB- denotes )
T17615 26127-26128 . denotes .
T17616 26128-26747 sentence denotes Figure 6 Histological and Immunochemical Examination of Thymus (A–D) P3 normal thymus. (E–G) Mild P3 mutant thymus. (H–K) Severe P3 mutant thymus. (L–O) P13 mutant thymus. Stained with H&E for histology (A, E, H, L), BrdU (B, I, M), β-catenin (C, F, J, N), and K14 (D, G, K, O). (B) Actively dividing thymocytes are visible at the superficial edge of cortex of normal P3 thymus. Note the progression of histological abnormalities in the mutant thymus from mild P3, severe P3 to P13 (A, E, H, L). Scale bars, 20 μm. The histological abnormalities of thymus were evident as early as P3 in KA mutants (Figure 6E and 6H).
T17617 26645-26648 DT denotes The
T17619 26649-26661 JJ denotes histological
T17618 26662-26675 NNS denotes abnormalities
T17621 26676-26678 IN denotes of
T17622 26679-26685 NN denotes thymus
T17620 26686-26690 VBD denotes were
T17623 26691-26698 JJ denotes evident
T17624 26699-26701 RB denotes as
T17625 26702-26707 RB denotes early
T17626 26708-26710 IN denotes as
T17627 26711-26713 NN denotes P3
T17628 26714-26716 IN denotes in
T17629 26717-26719 NN denotes KA
T17630 26720-26727 NNS denotes mutants
T17631 26728-26729 -LRB- denotes (
T17633 26729-26735 NN denotes Figure
T17632 26736-26738 NN denotes 6E
T17634 26739-26742 CC denotes and
T17635 26743-26745 NN denotes 6H
T17636 26745-26746 -RRB- denotes )
T17637 26746-26747 . denotes .
T17638 26747-26911 sentence denotes The thymus was made of two lobules as in the normal mice but the mutant thymus was significantly smaller in size than that of the age-matched controls (Figure 2G).
T17639 26748-26751 DT denotes The
T17640 26752-26758 NN denotes thymus
T17642 26759-26762 VBD denotes was
T17641 26763-26767 VBN denotes made
T17643 26768-26770 IN denotes of
T17644 26771-26774 CD denotes two
T17645 26775-26782 NNS denotes lobules
T17646 26783-26785 IN denotes as
T17647 26786-26788 IN denotes in
T17648 26789-26792 DT denotes the
T17650 26793-26799 JJ denotes normal
T17649 26800-26804 NNS denotes mice
T17651 26805-26808 CC denotes but
T17652 26809-26812 DT denotes the
T17654 26813-26819 JJ denotes mutant
T17653 26820-26826 NN denotes thymus
T17655 26827-26830 VBD denotes was
T17656 26831-26844 RB denotes significantly
T17657 26845-26852 JJR denotes smaller
T17658 26853-26855 IN denotes in
T17659 26856-26860 NN denotes size
T17660 26861-26865 IN denotes than
T17661 26866-26870 DT denotes that
T17662 26871-26873 IN denotes of
T17663 26874-26877 DT denotes the
T17665 26878-26881 NN denotes age
T17667 26881-26882 HYPH denotes -
T17666 26882-26889 JJ denotes matched
T17664 26890-26898 NNS denotes controls
T17668 26899-26900 -LRB- denotes (
T17670 26900-26906 NN denotes Figure
T17669 26907-26909 NN denotes 2G
T17671 26909-26910 -RRB- denotes )
T17672 26910-26911 . denotes .
T17673 26911-27071 sentence denotes Interestingly, variations in the phenotypic severity of the mutant pups at P3 were prominently reflected in the extent of histological abnormalities of thymus.
T17674 26912-26925 RB denotes Interestingly
T17676 26925-26927 , denotes ,
T17677 26927-26937 NNS denotes variations
T17678 26938-26940 IN denotes in
T17679 26941-26944 DT denotes the
T17681 26945-26955 JJ denotes phenotypic
T17680 26956-26964 NN denotes severity
T17682 26965-26967 IN denotes of
T17683 26968-26971 DT denotes the
T17685 26972-26978 JJ denotes mutant
T17684 26979-26983 NNS denotes pups
T17686 26984-26986 IN denotes at
T17687 26987-26989 NN denotes P3
T17688 26990-26994 VBD denotes were
T17689 26995-27006 RB denotes prominently
T17675 27007-27016 VBN denotes reflected
T17690 27017-27019 IN denotes in
T17691 27020-27023 DT denotes the
T17692 27024-27030 NN denotes extent
T17693 27031-27033 IN denotes of
T17694 27034-27046 JJ denotes histological
T17695 27047-27060 NNS denotes abnormalities
T17696 27061-27063 IN denotes of
T17697 27064-27070 NN denotes thymus
T17698 27070-27071 . denotes .
T17699 27071-27312 sentence denotes A P3 KA mutant pup that showed milder phenotype with a comparable body weight to its normal littermates (Figure 2A, M2) showed milder thymus abnormalities (Figure 6E) compared to its more severe mutant littermate (Figure 2A, M1; Figure 6H).
T17700 27072-27073 DT denotes A
T17702 27074-27076 NN denotes P3
T17703 27077-27079 NN denotes KA
T17704 27080-27086 JJ denotes mutant
T17701 27087-27090 NN denotes pup
T17706 27091-27095 WDT denotes that
T17707 27096-27102 VBD denotes showed
T17708 27103-27109 JJR denotes milder
T17709 27110-27119 NN denotes phenotype
T17710 27120-27124 IN denotes with
T17711 27125-27126 DT denotes a
T17713 27127-27137 JJ denotes comparable
T17714 27138-27142 NN denotes body
T17712 27143-27149 NN denotes weight
T17715 27150-27152 IN denotes to
T17716 27153-27156 PRP$ denotes its
T17718 27157-27163 JJ denotes normal
T17717 27164-27175 NNS denotes littermates
T17719 27176-27177 -LRB- denotes (
T17721 27177-27183 NN denotes Figure
T17720 27184-27186 NN denotes 2A
T17722 27186-27188 , denotes ,
T17723 27188-27190 NN denotes M2
T17724 27190-27191 -RRB- denotes )
T17705 27192-27198 VBD denotes showed
T17725 27199-27205 JJR denotes milder
T17727 27206-27212 NN denotes thymus
T17726 27213-27226 NNS denotes abnormalities
T17728 27227-27228 -LRB- denotes (
T17730 27228-27234 NN denotes Figure
T17729 27235-27237 NN denotes 6E
T17731 27237-27238 -RRB- denotes )
T17732 27239-27247 VBN denotes compared
T17733 27248-27250 IN denotes to
T17734 27251-27254 PRP$ denotes its
T17736 27255-27259 RBR denotes more
T17737 27260-27266 JJ denotes severe
T17738 27267-27273 JJ denotes mutant
T17735 27274-27284 NN denotes littermate
T17739 27285-27286 -LRB- denotes (
T17741 27286-27292 NN denotes Figure
T17740 27293-27295 NN denotes 2A
T17742 27295-27297 , denotes ,
T17743 27297-27299 NN denotes M1
T17744 27299-27300 : denotes ;
T17745 27301-27307 NN denotes Figure
T17746 27308-27310 NN denotes 6H
T17747 27310-27311 -RRB- denotes )
T17748 27311-27312 . denotes .
T17749 27312-27570 sentence denotes The milder P3 mutant thymus was already much smaller in size compared to those of normal littermates (data not shown) but two epithelial compartments of thymus were histologically still distinguishable, with colonization of thymocytes evident in the cortex.
T17750 27313-27316 DT denotes The
T17752 27317-27323 JJR denotes milder
T17753 27324-27326 NN denotes P3
T17754 27327-27333 JJ denotes mutant
T17751 27334-27340 NN denotes thymus
T17755 27341-27344 VBD denotes was
T17756 27345-27352 RB denotes already
T17757 27353-27357 RB denotes much
T17758 27358-27365 JJR denotes smaller
T17759 27366-27368 IN denotes in
T17760 27369-27373 NN denotes size
T17761 27374-27382 VBN denotes compared
T17762 27383-27385 IN denotes to
T17763 27386-27391 DT denotes those
T17764 27392-27394 IN denotes of
T17765 27395-27401 JJ denotes normal
T17766 27402-27413 NNS denotes littermates
T17767 27414-27415 -LRB- denotes (
T17769 27415-27419 NNS denotes data
T17770 27420-27423 RB denotes not
T17768 27424-27429 VBN denotes shown
T17771 27429-27430 -RRB- denotes )
T17772 27431-27434 CC denotes but
T17773 27435-27438 CD denotes two
T17775 27439-27449 JJ denotes epithelial
T17774 27450-27462 NNS denotes compartments
T17777 27463-27465 IN denotes of
T17778 27466-27472 NN denotes thymus
T17776 27473-27477 VBD denotes were
T17779 27478-27492 RB denotes histologically
T17780 27493-27498 RB denotes still
T17781 27499-27514 JJ denotes distinguishable
T17782 27514-27516 , denotes ,
T17783 27516-27520 IN denotes with
T17785 27521-27533 NN denotes colonization
T17786 27534-27536 IN denotes of
T17787 27537-27547 NNS denotes thymocytes
T17784 27548-27555 JJ denotes evident
T17788 27556-27558 IN denotes in
T17789 27559-27562 DT denotes the
T17790 27563-27569 NN denotes cortex
T17791 27569-27570 . denotes .
T17792 27570-27903 sentence denotes However, there were small populations of lightly stained cells by H&E extending from the edge of the outer cortex towards inner medulla (Figure 6E), and these cells showed intense nuclear β-catenin staining whereas the rest of the medullary cells showed diffuse β-catenin staining pattern similar to that of the control (Figure 6F).
T17793 27571-27578 RB denotes However
T17795 27578-27580 , denotes ,
T17796 27580-27585 EX denotes there
T17794 27586-27590 VBD denotes were
T17797 27591-27596 JJ denotes small
T17798 27597-27608 NNS denotes populations
T17799 27609-27611 IN denotes of
T17800 27612-27619 RB denotes lightly
T17801 27620-27627 VBN denotes stained
T17802 27628-27633 NNS denotes cells
T17803 27634-27636 IN denotes by
T17804 27637-27638 NN denotes H
T17805 27638-27639 CC denotes &
T17806 27639-27640 NN denotes E
T17807 27641-27650 VBG denotes extending
T17808 27651-27655 IN denotes from
T17809 27656-27659 DT denotes the
T17810 27660-27664 NN denotes edge
T17811 27665-27667 IN denotes of
T17812 27668-27671 DT denotes the
T17814 27672-27677 JJ denotes outer
T17813 27678-27684 NN denotes cortex
T17815 27685-27692 IN denotes towards
T17816 27693-27698 JJ denotes inner
T17817 27699-27706 NN denotes medulla
T17818 27707-27708 -LRB- denotes (
T17820 27708-27714 NN denotes Figure
T17819 27715-27717 NN denotes 6E
T17821 27717-27718 -RRB- denotes )
T17822 27718-27720 , denotes ,
T17823 27720-27723 CC denotes and
T17824 27724-27729 DT denotes these
T17825 27730-27735 NNS denotes cells
T17826 27736-27742 VBD denotes showed
T17827 27743-27750 JJ denotes intense
T17829 27751-27758 JJ denotes nuclear
T17830 27759-27760 NN denotes β
T17832 27760-27761 HYPH denotes -
T17831 27761-27768 NN denotes catenin
T17828 27769-27777 NN denotes staining
T17833 27778-27785 IN denotes whereas
T17835 27786-27789 DT denotes the
T17836 27790-27794 NN denotes rest
T17837 27795-27797 IN denotes of
T17838 27798-27801 DT denotes the
T17840 27802-27811 JJ denotes medullary
T17839 27812-27817 NNS denotes cells
T17834 27818-27824 VBD denotes showed
T17841 27825-27832 JJ denotes diffuse
T17843 27833-27834 NN denotes β
T17845 27834-27835 HYPH denotes -
T17844 27835-27842 NN denotes catenin
T17846 27843-27851 NN denotes staining
T17842 27852-27859 NN denotes pattern
T17847 27860-27867 JJ denotes similar
T17848 27868-27870 IN denotes to
T17849 27871-27875 DT denotes that
T17850 27876-27878 IN denotes of
T17851 27879-27882 DT denotes the
T17852 27883-27890 NN denotes control
T17853 27891-27892 -LRB- denotes (
T17855 27892-27898 NN denotes Figure
T17854 27899-27901 NN denotes 6F
T17856 27901-27902 -RRB- denotes )
T17857 27902-27903 . denotes .
T17858 27903-28019 sentence denotes Localization of K14 was limited to a few cells in the medulla and some overlapped with K8 localization (Figure 6G).
T17859 27904-27916 NN denotes Localization
T17861 27917-27919 IN denotes of
T17862 27920-27923 NN denotes K14
T17863 27924-27927 VBD denotes was
T17860 27928-27935 VBN denotes limited
T17864 27936-27938 IN denotes to
T17865 27939-27940 DT denotes a
T17867 27941-27944 JJ denotes few
T17866 27945-27950 NNS denotes cells
T17868 27951-27953 IN denotes in
T17869 27954-27957 DT denotes the
T17870 27958-27965 NN denotes medulla
T17871 27966-27969 CC denotes and
T17872 27970-27974 DT denotes some
T17873 27975-27985 VBN denotes overlapped
T17874 27986-27990 IN denotes with
T17875 27991-27993 NN denotes K8
T17876 27994-28006 NN denotes localization
T17877 28007-28008 -LRB- denotes (
T17879 28008-28014 NN denotes Figure
T17878 28015-28017 NN denotes 6G
T17880 28017-28018 -RRB- denotes )
T17881 28018-28019 . denotes .
T17882 28019-28211 sentence denotes In the other P3 mutant thymus the distinct thymic epithelial compartments have been lost completely, and only a few lymphocytes were remaining at the edges and some in the middle (Figure 6H).
T17883 28020-28022 IN denotes In
T17885 28023-28026 DT denotes the
T17887 28027-28032 JJ denotes other
T17888 28033-28035 NN denotes P3
T17889 28036-28042 JJ denotes mutant
T17886 28043-28049 NN denotes thymus
T17890 28050-28053 DT denotes the
T17892 28054-28062 JJ denotes distinct
T17893 28063-28069 JJ denotes thymic
T17894 28070-28080 JJ denotes epithelial
T17891 28081-28093 NNS denotes compartments
T17895 28094-28098 VBP denotes have
T17896 28099-28103 VBN denotes been
T17884 28104-28108 VBN denotes lost
T17897 28109-28119 RB denotes completely
T17898 28119-28121 , denotes ,
T17899 28121-28124 CC denotes and
T17900 28125-28129 RB denotes only
T17902 28130-28131 DT denotes a
T17903 28132-28135 JJ denotes few
T17901 28136-28147 NNS denotes lymphocytes
T17905 28148-28152 VBD denotes were
T17904 28153-28162 VBG denotes remaining
T17906 28163-28165 IN denotes at
T17907 28166-28169 DT denotes the
T17908 28170-28175 NNS denotes edges
T17909 28176-28179 CC denotes and
T17910 28180-28184 DT denotes some
T17911 28185-28187 IN denotes in
T17912 28188-28191 DT denotes the
T17913 28192-28198 JJ denotes middle
T17914 28199-28200 -LRB- denotes (
T17916 28200-28206 NN denotes Figure
T17915 28207-28209 NN denotes 6H
T17917 28209-28210 -RRB- denotes )
T17918 28210-28211 . denotes .
T17919 28211-28395 sentence denotes Proliferative activities were no longer observed in thymocytes as prominently as in the normal thymus, but the epithelial cells seemed to be forming concentric structures (Figure 6I).
T17920 28212-28225 JJ denotes Proliferative
T17921 28226-28236 NNS denotes activities
T17923 28237-28241 VBD denotes were
T17924 28242-28244 RB denotes no
T17925 28245-28251 RBR denotes longer
T17922 28252-28260 VBN denotes observed
T17926 28261-28263 IN denotes in
T17927 28264-28274 NNS denotes thymocytes
T17928 28275-28277 RB denotes as
T17929 28278-28289 RB denotes prominently
T17930 28290-28292 IN denotes as
T17931 28293-28295 IN denotes in
T17932 28296-28299 DT denotes the
T17934 28300-28306 JJ denotes normal
T17933 28307-28313 NN denotes thymus
T17935 28313-28315 , denotes ,
T17936 28315-28318 CC denotes but
T17937 28319-28322 DT denotes the
T17939 28323-28333 JJ denotes epithelial
T17938 28334-28339 NNS denotes cells
T17940 28340-28346 VBD denotes seemed
T17941 28347-28349 TO denotes to
T17943 28350-28352 VB denotes be
T17942 28353-28360 VBG denotes forming
T17944 28361-28371 JJ denotes concentric
T17945 28372-28382 NNS denotes structures
T17946 28383-28384 -LRB- denotes (
T17948 28384-28390 NN denotes Figure
T17947 28391-28393 NN denotes 6I
T17949 28393-28394 -RRB- denotes )
T17950 28394-28395 . denotes .
T17951 28395-28546 sentence denotes Unlike in the normal or mild mutant thymi, the severe P3 mutant thymus showed extensive K14 expression that overlapped with K8 expression (Figure 6K).
T17952 28396-28402 IN denotes Unlike
T17954 28403-28405 IN denotes in
T17955 28406-28409 DT denotes the
T17957 28410-28416 JJ denotes normal
T17958 28417-28419 CC denotes or
T17959 28420-28424 JJ denotes mild
T17960 28425-28431 JJ denotes mutant
T17956 28432-28437 NNS denotes thymi
T17961 28437-28439 , denotes ,
T17962 28439-28442 DT denotes the
T17964 28443-28449 JJ denotes severe
T17965 28450-28452 NN denotes P3
T17966 28453-28459 JJ denotes mutant
T17963 28460-28466 NN denotes thymus
T17953 28467-28473 VBD denotes showed
T17967 28474-28483 JJ denotes extensive
T17969 28484-28487 NN denotes K14
T17968 28488-28498 NN denotes expression
T17970 28499-28503 WDT denotes that
T17971 28504-28514 VBD denotes overlapped
T17972 28515-28519 IN denotes with
T17973 28520-28522 NN denotes K8
T17974 28523-28533 NN denotes expression
T17975 28534-28535 -LRB- denotes (
T17977 28535-28541 NN denotes Figure
T17976 28542-28544 NN denotes 6K
T17978 28544-28545 -RRB- denotes )
T17979 28545-28546 . denotes .
T17980 28546-28741 sentence denotes These cells were more like basal cells of the skin than TECs and were adjacent to the most immature looking cells that were showing strong nuclear and cytoplasmic β-catenin staining (Figure 6J).
T17981 28547-28552 DT denotes These
T17982 28553-28558 NNS denotes cells
T17983 28559-28563 VBD denotes were
T17984 28564-28568 RBR denotes more
T17985 28569-28573 IN denotes like
T17986 28574-28579 JJ denotes basal
T17987 28580-28585 NNS denotes cells
T17988 28586-28588 IN denotes of
T17989 28589-28592 DT denotes the
T17990 28593-28597 NN denotes skin
T17991 28598-28602 IN denotes than
T17992 28603-28607 NNS denotes TECs
T17993 28608-28611 CC denotes and
T17994 28612-28616 VBD denotes were
T17995 28617-28625 JJ denotes adjacent
T17996 28626-28628 IN denotes to
T17997 28629-28632 DT denotes the
T17999 28633-28637 RBS denotes most
T18000 28638-28646 JJ denotes immature
T18001 28647-28654 VBG denotes looking
T17998 28655-28660 NNS denotes cells
T18002 28661-28665 WDT denotes that
T18004 28666-28670 VBD denotes were
T18003 28671-28678 VBG denotes showing
T18005 28679-28685 JJ denotes strong
T18007 28686-28693 JJ denotes nuclear
T18008 28694-28697 CC denotes and
T18009 28698-28709 JJ denotes cytoplasmic
T18010 28710-28711 NN denotes β
T18012 28711-28712 HYPH denotes -
T18011 28712-28719 NN denotes catenin
T18006 28720-28728 NN denotes staining
T18013 28729-28730 -LRB- denotes (
T18015 28730-28736 NN denotes Figure
T18014 28737-28739 NN denotes 6J
T18016 28739-28740 -RRB- denotes )
T18017 28740-28741 . denotes .
T18018 28741-28838 sentence denotes The nuclear staining of β-catenin was not observed in the normal age-matched thymus (Figure 6C).
T18019 28742-28745 DT denotes The
T18021 28746-28753 JJ denotes nuclear
T18020 28754-28762 NN denotes staining
T18023 28763-28765 IN denotes of
T18024 28766-28767 NN denotes β
T18026 28767-28768 HYPH denotes -
T18025 28768-28775 NN denotes catenin
T18027 28776-28779 VBD denotes was
T18028 28780-28783 RB denotes not
T18022 28784-28792 VBN denotes observed
T18029 28793-28795 IN denotes in
T18030 28796-28799 DT denotes the
T18032 28800-28806 JJ denotes normal
T18033 28807-28810 NN denotes age
T18035 28810-28811 HYPH denotes -
T18034 28811-28818 JJ denotes matched
T18031 28819-28825 NN denotes thymus
T18036 28826-28827 -LRB- denotes (
T18038 28827-28833 NN denotes Figure
T18037 28834-28836 NN denotes 6C
T18039 28836-28837 -RRB- denotes )
T18040 28837-28838 . denotes .
T18041 28838-28936 sentence denotes Most notably, the K14 and β-catenin staining patterns were mutually exclusive (Figure 6J and 6K).
T18042 28839-28843 RBS denotes Most
T18043 28844-28851 RB denotes notably
T18045 28851-28853 , denotes ,
T18046 28853-28856 DT denotes the
T18048 28857-28860 NN denotes K14
T18049 28861-28864 CC denotes and
T18050 28865-28866 NN denotes β
T18052 28866-28867 HYPH denotes -
T18051 28867-28874 NN denotes catenin
T18053 28875-28883 NN denotes staining
T18047 28884-28892 NNS denotes patterns
T18044 28893-28897 VBD denotes were
T18054 28898-28906 RB denotes mutually
T18055 28907-28916 JJ denotes exclusive
T18056 28917-28918 -LRB- denotes (
T18058 28918-28924 NN denotes Figure
T18057 28925-28927 NN denotes 6J
T18059 28928-28931 CC denotes and
T18060 28932-28934 NN denotes 6K
T18061 28934-28935 -RRB- denotes )
T18062 28935-28936 . denotes .
T18063 28936-29160 sentence denotes At P10–P13, the mutant thymus consisted of numerous enlarged Hassall's corpuscle–like structures, made of arrays of K14- and K8-expressing keratinizing epithelial cells surrounding large keratin deposits (Figure 6L and 6O).
T18064 28937-28939 IN denotes At
T18066 28940-28943 NN denotes P10
T18067 28943-28944 SYM denotes –
T18068 28944-28947 NN denotes P13
T18069 28947-28949 , denotes ,
T18070 28949-28952 DT denotes the
T18072 28953-28959 JJ denotes mutant
T18071 28960-28966 NN denotes thymus
T18065 28967-28976 VBD denotes consisted
T18073 28977-28979 IN denotes of
T18074 28980-28988 JJ denotes numerous
T18076 28989-28997 VBN denotes enlarged
T18077 28998-29005 NNP denotes Hassall
T18078 29005-29007 POS denotes 's
T18079 29008-29017 NN denotes corpuscle
T18081 29017-29018 HYPH denotes –
T18080 29018-29022 JJ denotes like
T18075 29023-29033 NNS denotes structures
T18082 29033-29035 , denotes ,
T18083 29035-29039 VBN denotes made
T18084 29040-29042 IN denotes of
T18085 29043-29049 NNS denotes arrays
T18086 29050-29052 IN denotes of
T18087 29053-29056 NN denotes K14
T18089 29056-29057 HYPH denotes -
T18090 29058-29061 CC denotes and
T18091 29062-29064 NN denotes K8
T18092 29064-29065 HYPH denotes -
T18088 29065-29075 VBG denotes expressing
T18094 29076-29088 VBG denotes keratinizing
T18095 29089-29099 JJ denotes epithelial
T18093 29100-29105 NNS denotes cells
T18096 29106-29117 VBG denotes surrounding
T18097 29118-29123 JJ denotes large
T18099 29124-29131 NN denotes keratin
T18098 29132-29140 NNS denotes deposits
T18100 29141-29142 -LRB- denotes (
T18102 29142-29148 NN denotes Figure
T18101 29149-29151 NN denotes 6L
T18103 29152-29155 CC denotes and
T18104 29156-29158 NN denotes 6O
T18105 29158-29159 -RRB- denotes )
T18106 29159-29160 . denotes .
T18107 29160-29317 sentence denotes There were numerous neutrophils and macrophages infiltrating the thymus in response to these keratins; hence, these structures could be called pyogranuloma.
T18108 29161-29166 EX denotes There
T18109 29167-29171 VBD denotes were
T18111 29172-29180 JJ denotes numerous
T18112 29181-29192 NNS denotes neutrophils
T18113 29193-29196 CC denotes and
T18114 29197-29208 NNS denotes macrophages
T18115 29209-29221 VBG denotes infiltrating
T18116 29222-29225 DT denotes the
T18117 29226-29232 NN denotes thymus
T18118 29233-29235 IN denotes in
T18119 29236-29244 NN denotes response
T18120 29245-29247 IN denotes to
T18121 29248-29253 DT denotes these
T18122 29254-29262 NNS denotes keratins
T18123 29262-29263 : denotes ;
T18124 29264-29269 RB denotes hence
T18125 29269-29271 , denotes ,
T18126 29271-29276 DT denotes these
T18127 29277-29287 NNS denotes structures
T18128 29288-29293 MD denotes could
T18129 29294-29296 VB denotes be
T18110 29297-29303 VBN denotes called
T18130 29304-29316 NN denotes pyogranuloma
T18131 29316-29317 . denotes .
T18132 29317-29534 sentence denotes Varying degrees of differentiation-specific markers depending on the age of mice, in this case K1 and involucrin that are normally present only in Hassall's corpuscles, were also detected in mutant thymi (Figure S3).
T18133 29318-29325 VBG denotes Varying
T18134 29326-29333 NNS denotes degrees
T18136 29334-29336 IN denotes of
T18137 29337-29352 NN denotes differentiation
T18139 29352-29353 HYPH denotes -
T18138 29353-29361 JJ denotes specific
T18140 29362-29369 NNS denotes markers
T18141 29370-29379 VBG denotes depending
T18142 29380-29382 IN denotes on
T18143 29383-29386 DT denotes the
T18144 29387-29390 NN denotes age
T18145 29391-29393 IN denotes of
T18146 29394-29398 NNS denotes mice
T18147 29398-29400 , denotes ,
T18148 29400-29402 IN denotes in
T18150 29403-29407 DT denotes this
T18151 29408-29412 NN denotes case
T18149 29413-29415 NN denotes K1
T18152 29416-29419 CC denotes and
T18153 29420-29430 NN denotes involucrin
T18154 29431-29435 WDT denotes that
T18155 29436-29439 VBP denotes are
T18156 29440-29448 RB denotes normally
T18157 29449-29456 JJ denotes present
T18158 29457-29461 RB denotes only
T18159 29462-29464 IN denotes in
T18160 29465-29472 NNP denotes Hassall
T18162 29472-29474 POS denotes 's
T18161 29475-29485 NNS denotes corpuscles
T18163 29485-29487 , denotes ,
T18164 29487-29491 VBD denotes were
T18165 29492-29496 RB denotes also
T18135 29497-29505 VBN denotes detected
T18166 29506-29508 IN denotes in
T18167 29509-29515 JJ denotes mutant
T18168 29516-29521 NNS denotes thymi
T18169 29522-29523 -LRB- denotes (
T18171 29523-29529 NN denotes Figure
T18170 29530-29532 NN denotes S3
T18172 29532-29533 -RRB- denotes )
T18173 29533-29534 . denotes .
T18174 29534-29579 sentence denotes In these mice no thymocytes were detectable.
T18175 29535-29537 IN denotes In
T18177 29538-29543 DT denotes these
T18178 29544-29548 NNS denotes mice
T18179 29549-29551 DT denotes no
T18180 29552-29562 NNS denotes thymocytes
T18176 29563-29567 VBD denotes were
T18181 29568-29578 JJ denotes detectable
T18182 29578-29579 . denotes .
T18183 29579-29727 sentence denotes BrdU incorporation was only observed in very few keratinizing epithelial cells, looking somewhat similar to the pattern of mature skin (Figure 6M).
T18184 29580-29584 NN denotes BrdU
T18185 29585-29598 NN denotes incorporation
T18187 29599-29602 VBD denotes was
T18188 29603-29607 RB denotes only
T18189 29608-29616 VBN denotes observed
T18190 29617-29619 IN denotes in
T18191 29620-29624 RB denotes very
T18192 29625-29628 JJ denotes few
T18194 29629-29641 VBG denotes keratinizing
T18195 29642-29652 JJ denotes epithelial
T18193 29653-29658 NNS denotes cells
T18196 29658-29660 , denotes ,
T18186 29660-29667 VBG denotes looking
T18197 29668-29676 RB denotes somewhat
T18198 29677-29684 JJ denotes similar
T18199 29685-29687 IN denotes to
T18200 29688-29691 DT denotes the
T18201 29692-29699 NN denotes pattern
T18202 29700-29702 IN denotes of
T18203 29703-29709 JJ denotes mature
T18204 29710-29714 NN denotes skin
T18205 29715-29716 -LRB- denotes (
T18207 29716-29722 NN denotes Figure
T18206 29723-29725 NN denotes 6M
T18208 29725-29726 -RRB- denotes )
T18209 29726-29727 . denotes .
T18210 29727-29893 sentence denotes The diffuse expression of β-catenin was also present in these epithelial cells, and at this age fewer cells were positive for nuclear β-catenin staining (Figure 6N).
T18211 29728-29731 DT denotes The
T18213 29732-29739 JJ denotes diffuse
T18212 29740-29750 NN denotes expression
T18215 29751-29753 IN denotes of
T18216 29754-29755 NN denotes β
T18218 29755-29756 HYPH denotes -
T18217 29756-29763 NN denotes catenin
T18214 29764-29767 VBD denotes was
T18219 29768-29772 RB denotes also
T18220 29773-29780 JJ denotes present
T18221 29781-29783 IN denotes in
T18222 29784-29789 DT denotes these
T18224 29790-29800 JJ denotes epithelial
T18223 29801-29806 NNS denotes cells
T18225 29806-29808 , denotes ,
T18226 29808-29811 CC denotes and
T18227 29812-29814 IN denotes at
T18229 29815-29819 DT denotes this
T18230 29820-29823 NN denotes age
T18231 29824-29829 JJR denotes fewer
T18232 29830-29835 NNS denotes cells
T18228 29836-29840 VBD denotes were
T18233 29841-29849 JJ denotes positive
T18234 29850-29853 IN denotes for
T18235 29854-29861 JJ denotes nuclear
T18237 29862-29863 NN denotes β
T18239 29863-29864 HYPH denotes -
T18238 29864-29871 NN denotes catenin
T18236 29872-29880 NN denotes staining
T18240 29881-29882 -LRB- denotes (
T18242 29882-29888 NN denotes Figure
T18241 29889-29891 NN denotes 6N
T18243 29891-29892 -RRB- denotes )
T18244 29892-29893 . denotes .
T18245 29893-30054 sentence denotes As in the younger mutant mice, however, nuclear localization of β-catenin was only observed in K14-negative cells that looked like undifferentiated basal cells.
T18246 29894-29896 IN denotes As
T18248 29897-29899 IN denotes in
T18249 29900-29903 DT denotes the
T18251 29904-29911 JJR denotes younger
T18252 29912-29918 JJ denotes mutant
T18250 29919-29923 NNS denotes mice
T18253 29923-29925 , denotes ,
T18254 29925-29932 RB denotes however
T18255 29932-29934 , denotes ,
T18256 29934-29941 JJ denotes nuclear
T18257 29942-29954 NN denotes localization
T18258 29955-29957 IN denotes of
T18259 29958-29959 NN denotes β
T18261 29959-29960 HYPH denotes -
T18260 29960-29967 NN denotes catenin
T18262 29968-29971 VBD denotes was
T18263 29972-29976 RB denotes only
T18247 29977-29985 VBN denotes observed
T18264 29986-29988 IN denotes in
T18265 29989-29992 NN denotes K14
T18267 29992-29993 HYPH denotes -
T18266 29993-30001 JJ denotes negative
T18268 30002-30007 NNS denotes cells
T18269 30008-30012 WDT denotes that
T18270 30013-30019 VBD denotes looked
T18271 30020-30024 IN denotes like
T18272 30025-30041 JJ denotes undifferentiated
T18274 30042-30047 JJ denotes basal
T18273 30048-30053 NNS denotes cells
T18275 30053-30054 . denotes .
T18276 30054-30313 sentence denotes In older P17 mice, the histopathology and keratin expression pattern of the mutant thymus was similar to that of P13 except for the fact that β-catenin expression became increasingly diffuse and appeared to colocalize with K8/K14 expression (data not shown).
T18277 30055-30057 IN denotes In
T18279 30058-30063 JJR denotes older
T18281 30064-30067 NN denotes P17
T18280 30068-30072 NNS denotes mice
T18282 30072-30074 , denotes ,
T18283 30074-30077 DT denotes the
T18284 30078-30092 NN denotes histopathology
T18285 30093-30096 CC denotes and
T18286 30097-30104 NN denotes keratin
T18288 30105-30115 NN denotes expression
T18287 30116-30123 NN denotes pattern
T18289 30124-30126 IN denotes of
T18290 30127-30130 DT denotes the
T18292 30131-30137 JJ denotes mutant
T18291 30138-30144 NN denotes thymus
T18278 30145-30148 VBD denotes was
T18293 30149-30156 JJ denotes similar
T18294 30157-30159 IN denotes to
T18295 30160-30164 DT denotes that
T18296 30165-30167 IN denotes of
T18297 30168-30171 NN denotes P13
T18298 30172-30178 IN denotes except
T18299 30179-30182 IN denotes for
T18300 30183-30186 DT denotes the
T18301 30187-30191 NN denotes fact
T18302 30192-30196 IN denotes that
T18304 30197-30198 NN denotes β
T18306 30198-30199 HYPH denotes -
T18305 30199-30206 NN denotes catenin
T18307 30207-30217 NN denotes expression
T18303 30218-30224 VBD denotes became
T18308 30225-30237 RB denotes increasingly
T18309 30238-30245 JJ denotes diffuse
T18310 30246-30249 CC denotes and
T18311 30250-30258 VBD denotes appeared
T18312 30259-30261 TO denotes to
T18313 30262-30272 VB denotes colocalize
T18314 30273-30277 IN denotes with
T18315 30278-30280 NN denotes K8
T18317 30280-30281 HYPH denotes /
T18316 30281-30284 NN denotes K14
T18318 30285-30295 NN denotes expression
T18319 30296-30297 -LRB- denotes (
T18321 30297-30301 NNS denotes data
T18322 30302-30305 RB denotes not
T18320 30306-30311 VBN denotes shown
T18323 30311-30312 -RRB- denotes )
T18324 30312-30313 . denotes .
T18325 30313-30382 sentence denotes This coincided with fewer immature cells in the older mutant thymus.
T18326 30314-30318 DT denotes This
T18327 30319-30328 VBD denotes coincided
T18328 30329-30333 IN denotes with
T18329 30334-30339 JJR denotes fewer
T18331 30340-30348 JJ denotes immature
T18330 30349-30354 NNS denotes cells
T18332 30355-30357 IN denotes in
T18333 30358-30361 DT denotes the
T18335 30362-30367 JJR denotes older
T18336 30368-30374 JJ denotes mutant
T18334 30375-30381 NN denotes thymus
T18337 30381-30382 . denotes .
T18338 30382-30667 sentence denotes Collectively, these results suggest that loss of Apc and consequent stabilization of β-catenin in K14-expressing TECs lead to their aberrant proliferation and differentiation to keratinocytes, causing massive squamous metaplasia, rather than to form either medullary or cortical TECs.
T18339 30383-30395 RB denotes Collectively
T18341 30395-30397 , denotes ,
T18342 30397-30402 DT denotes these
T18343 30403-30410 NNS denotes results
T18340 30411-30418 VBP denotes suggest
T18344 30419-30423 IN denotes that
T18346 30424-30428 NN denotes loss
T18347 30429-30431 IN denotes of
T18348 30432-30435 NN denotes Apc
T18349 30436-30439 CC denotes and
T18350 30440-30450 JJ denotes consequent
T18351 30451-30464 NN denotes stabilization
T18352 30465-30467 IN denotes of
T18353 30468-30469 NN denotes β
T18355 30469-30470 HYPH denotes -
T18354 30470-30477 NN denotes catenin
T18356 30478-30480 IN denotes in
T18357 30481-30484 NN denotes K14
T18359 30484-30485 HYPH denotes -
T18358 30485-30495 VBG denotes expressing
T18360 30496-30500 NNS denotes TECs
T18345 30501-30505 VBP denotes lead
T18361 30506-30508 IN denotes to
T18362 30509-30514 PRP$ denotes their
T18364 30515-30523 JJ denotes aberrant
T18363 30524-30537 NN denotes proliferation
T18365 30538-30541 CC denotes and
T18366 30542-30557 NN denotes differentiation
T18367 30558-30560 IN denotes to
T18368 30561-30574 NNS denotes keratinocytes
T18369 30574-30576 , denotes ,
T18370 30576-30583 VBG denotes causing
T18371 30584-30591 JJ denotes massive
T18373 30592-30600 JJ denotes squamous
T18372 30601-30611 NN denotes metaplasia
T18374 30611-30613 , denotes ,
T18375 30613-30619 IN denotes rather
T18376 30620-30624 IN denotes than
T18377 30625-30627 TO denotes to
T18378 30628-30632 VB denotes form
T18379 30633-30639 CC denotes either
T18380 30640-30649 JJ denotes medullary
T18382 30650-30652 CC denotes or
T18383 30653-30661 JJ denotes cortical
T18381 30662-30666 NNS denotes TECs
T18384 30666-30667 . denotes .
T18385 30667-30788 sentence denotes Loss of proper TEC compartments consequently resulted in loss of thymocytes for maturation and the mice to be “athymic.”
T18386 30668-30672 NN denotes Loss
T18388 30673-30675 IN denotes of
T18389 30676-30682 JJ denotes proper
T18391 30683-30686 NN denotes TEC
T18390 30687-30699 NNS denotes compartments
T18392 30700-30712 RB denotes consequently
T18387 30713-30721 VBD denotes resulted
T18393 30722-30724 IN denotes in
T18394 30725-30729 NN denotes loss
T18395 30730-30732 IN denotes of
T18396 30733-30743 NNS denotes thymocytes
T18397 30744-30747 IN denotes for
T18398 30748-30758 NN denotes maturation
T18399 30759-30762 CC denotes and
T18400 30763-30766 DT denotes the
T18401 30767-30771 NNS denotes mice
T18403 30772-30774 TO denotes to
T18402 30775-30777 VB denotes be
T18404 30778-30779 `` denotes “
T18405 30779-30786 JJ denotes athymic
T18406 30786-30787 . denotes .
T18407 30787-30788 '' denotes ”
R1000 T4684 T4640 parataxis 13,constructed
R1001 T4685 T4684 nummod 12,13
R1002 T4686 T4684 punct ",",13
R1003 T4687 T4684 punct ],13
R1004 T4688 T4689 punct (,N
R1005 T4689 T4640 parataxis N,constructed
R1006 T4690 T4691 compound Figure,1A
R1007 T4691 T4689 dep 1A,N
R1008 T4692 T4689 punct ", ",N
R1009 T4693 T4694 compound ApcCKON,allele
R1010 T4694 T4689 dep allele,N
R1011 T4695 T4689 punct ", ",N
R1012 T4696 T4689 prep for,N
R1013 T4697 T4698 compound neomycin,cassette
R1014 T4698 T4696 pobj cassette,for
R1015 T4699 T4689 punct ),N
R1016 T4700 T4640 punct .,constructed
R1017 T4702 T4703 det A,cassette
R1018 T4703 T4707 nsubjpass cassette,inserted
R1019 T4704 T4705 compound PGK,neomycin
R1020 T4705 T4703 compound neomycin,cassette
R1021 T4706 T4705 punct -,neomycin
R1022 T4708 T4707 auxpass was,inserted
R1023 T4709 T4707 prep in,inserted
R1024 T4710 T4711 det the,orientation
R1025 T4711 T4709 pobj orientation,in
R1026 T4712 T4711 amod same,orientation
R1027 T4713 T4711 amod transcriptional,orientation
R1028 T4714 T4711 prep as,orientation
R1029 T4715 T4714 pobj Apc,as
R1030 T4716 T4707 prep in,inserted
R1031 T4717 T4716 pobj intron,in
R1032 T4718 T4717 nummod 14,intron
R1033 T4719 T4717 prep of,intron
R1034 T4720 T4721 det the,gene
R1035 T4721 T4719 pobj gene,of
R1036 T4722 T4721 amod endogenous,gene
R1037 T4723 T4707 punct .,inserted
R1038 T4725 T4726 det The,sites
R1039 T4726 T4730 nsubjpass sites,used
R1040 T4727 T4726 nmod loxP,sites
R1041 T4728 T4727 cc and,loxP
R1042 T4729 T4727 conj FRT,loxP
R1043 T4731 T4730 auxpass were,used
R1044 T4732 T4733 aux to,aid
R1045 T4733 T4730 advcl aid,used
R1046 T4734 T4735 amod unidirectional,recombination
R1047 T4735 T4733 dobj recombination,aid
R1048 T4736 T4737 punct [,13
R1049 T4737 T4730 parataxis 13,used
R1050 T4738 T4737 nummod 12,13
R1051 T4739 T4737 punct ",",13
R1052 T4740 T4737 punct ],13
R1053 T4741 T4730 punct .,used
R1054 T4743 T4744 nummod Two,lines
R1055 T4744 T4746 nsubjpass lines,generated
R1056 T4745 T4744 compound mouse,lines
R1057 T4747 T4744 acl containing,lines
R1058 T4748 T4749 det the,modification
R1059 T4749 T4747 dobj modification,containing
R1060 T4750 T4749 amod same,modification
R1061 T4751 T4746 auxpass were,generated
R1062 T4752 T4746 prep from,generated
R1063 T4753 T4754 nummod two,clones
R1064 T4754 T4752 pobj clones,from
R1065 T4755 T4754 amod independent,clones
R1066 T4756 T4754 compound ES,clones
R1067 T4757 T4758 aux to,ensure
R1068 T4758 T4746 advcl ensure,generated
R1069 T4759 T4760 mark that,behave
R1070 T4760 T4758 ccomp behave,ensure
R1071 T4761 T4762 det these,lines
R1072 T4762 T4760 nsubj lines,behave
R1073 T4763 T4762 nummod two,lines
R1074 T4764 T4760 prep in,behave
R1075 T4765 T4766 det the,way
R1076 T4766 T4764 pobj way,in
R1077 T4767 T4766 amod same,way
R1078 T4768 T4746 punct .,generated
R1079 T4770 T4771 det These,mice
R1080 T4771 T4775 nsubjpass mice,crossed
R1081 T4772 T4771 nmod ApcCKON,mice
R1082 T4773 T4772 punct /,ApcCKON
R1083 T4774 T4772 punct +,ApcCKON
R1084 T4776 T4775 auxpass were,crossed
R1085 T4777 T4775 prep with,crossed
R1086 T4778 T4779 compound FLPe,deleter
R1087 T4779 T4777 pobj deleter,with
R1088 T4780 T4779 punct -,deleter
R1089 T4781 T4782 aux to,generate
R1090 T4782 T4775 advcl generate,crossed
R1091 T4783 T4784 nmod ApcCKO,mice
R1092 T4784 T4782 dobj mice,generate
R1093 T4785 T4783 punct /,ApcCKO
R1094 T4786 T4783 punct +,ApcCKO
R1095 T4787 T4788 dep that,were
R1096 T4788 T4784 relcl were,mice
R1097 T4789 T4788 acomp heterozygous,were
R1098 T4790 T4789 prep for,heterozygous
R1099 T4791 T4792 det the,allele
R1100 T4792 T4790 pobj allele,for
R1101 T4793 T4792 amod final,allele
R1102 T4794 T4795 npadvmod Apc,conditional
R1103 T4795 T4792 amod conditional,allele
R1104 T4796 T4797 punct (,ApcCKO
R1105 T4797 T4795 parataxis ApcCKO,conditional
R1106 T4798 T4797 punct ),ApcCKO
R1107 T4799 T4800 dep that,removed
R1108 T4800 T4792 relcl removed,allele
R1109 T4801 T4802 det the,cassette
R1110 T4802 T4800 dobj cassette,removed
R1111 T4803 T4804 compound PGK,neomycin
R1112 T4804 T4802 compound neomycin,cassette
R1113 T4805 T4804 punct -,neomycin
R1114 T4806 T4800 cc and,removed
R1115 T4807 T4800 conj contains,removed
R1116 T4808 T4809 advmod only,sites
R1117 T4809 T4807 dobj sites,contains
R1118 T4810 T4809 det the,sites
R1119 T4811 T4809 compound loxP,sites
R1120 T4812 T4807 prep in,contains
R1121 T4813 T4814 det the,introns
R1122 T4814 T4812 pobj introns,in
R1123 T4815 T4814 acl flanking,introns
R1124 T4816 T4815 dobj exon,flanking
R1125 T4817 T4816 nummod 14,exon
R1126 T4818 T4775 punct .,crossed
R1127 T4820 T4821 aux To,assess
R1128 T4821 T4822 advcl assess,crossed
R1129 T4823 T4824 det the,effect
R1130 T4824 T4821 dobj effect,assess
R1131 T4825 T4824 prep of,effect
R1132 T4826 T4825 pcomp deleting,of
R1133 T4827 T4826 dobj exon,deleting
R1134 T4828 T4827 nummod 14,exon
R1135 T4829 T4821 prep in,assess
R1136 T4830 T4829 pobj mice,in
R1137 T4831 T4822 punct ", ",crossed
R1138 T4832 T4833 det both,lines
R1139 T4833 T4822 nsubjpass lines,crossed
R1140 T4834 T4833 prep of,lines
R1141 T4835 T4836 nmod ApcCKO,mice
R1142 T4836 T4834 pobj mice,of
R1143 T4837 T4835 punct /,ApcCKO
R1144 T4838 T4835 punct +,ApcCKO
R1145 T4839 T4822 auxpass were,crossed
R1146 T4840 T4822 prep with,crossed
R1147 T4841 T4842 det the,deleter
R1148 T4842 T4840 pobj deleter,with
R1149 T4843 T4842 compound Cre,deleter
R1150 T4844 T4842 punct -,deleter
R1151 T4845 T4846 aux to,generate
R1152 T4846 T4822 advcl generate,crossed
R1153 T4847 T4848 det the,line
R1154 T4848 T4846 dobj line,generate
R1155 T4849 T4850 compound germline,knockout
R1156 T4850 T4848 compound knockout,line
R1157 T4851 T4848 prep of,line
R1158 T4852 T4851 pobj Apc,of
R1159 T4853 T4848 punct ", ",line
R1160 T4854 T4848 acl designated,line
R1161 T4855 T4854 oprd ApcΔ580,designated
R1162 T4856 T4855 punct /,ApcΔ580
R1163 T4857 T4855 punct +,ApcΔ580
R1164 T4858 T4822 punct .,crossed
R1165 T4860 T4861 det The,allele
R1166 T4861 T4863 nsubj allele,lacks
R1167 T4862 T4861 amod mutant,allele
R1168 T4864 T4861 punct (,allele
R1169 T4865 T4861 appos ApcΔ580,allele
R1170 T4866 T4863 punct ),lacks
R1171 T4867 T4863 dobj exon,lacks
R1172 T4868 T4867 nummod 14,exon
R1173 T4869 T4870 punct (,1A
R1174 T4870 T4863 parataxis 1A,lacks
R1175 T4871 T4870 compound Figure,1A
R1176 T4872 T4870 punct ),1A
R1177 T4873 T4863 punct .,lacks
R1178 T4875 T4876 det The,transcript
R1179 T4876 T4877 nsubj transcript,results
R1180 T4878 T4876 prep from,transcript
R1181 T4879 T4878 pobj loss,from
R1182 T4880 T4879 prep of,loss
R1183 T4881 T4880 pobj exon,of
R1184 T4882 T4881 nummod 14,exon
R1185 T4883 T4877 prep in,results
R1186 T4884 T4885 det a,shift
R1187 T4885 T4883 pobj shift,in
R1188 T4886 T4885 prep in,shift
R1189 T4887 T4888 det the,frame
R1190 T4888 T4886 pobj frame,in
R1191 T4889 T4888 amod normal,frame
R1192 T4890 T4888 compound reading,frame
R1193 T4891 T4877 punct ", ",results
R1194 T4892 T4877 advcl resulting,results
R1195 T4893 T4892 prep in,resulting
R1196 T4894 T4895 det a,codon
R1197 T4895 T4893 pobj codon,in
R1198 T4896 T4895 amod premature,codon
R1199 T4897 T4898 compound chain,termination
R1200 T4898 T4895 compound termination,codon
R1201 T4899 T4900 dep which,result
R1202 T4900 T4895 relcl result,codon
R1203 T4901 T4900 punct ", ",result
R1204 T4902 T4903 mark if,utilized
R1205 T4903 T4900 advcl utilized,result
R1206 T4904 T4900 punct ", ",result
R1207 T4905 T4900 aux would,result
R1208 T4906 T4900 prep in,result
R1209 T4907 T4908 det a,polypeptide
R1210 T4908 T4906 pobj polypeptide,in
R1211 T4909 T4908 amod truncated,polypeptide
R1212 T4910 T4911 dep that,is
R1213 T4911 T4908 relcl is,polypeptide
R1214 T4912 T4913 nummod 605,aa
R1215 T4913 T4911 attr aa,is
R1216 T4914 T4913 prep in,aa
R1217 T4915 T4914 pobj length,in
R1218 T4916 T4913 punct ", ",aa
R1219 T4917 T4918 prep of,correspond
R1220 T4918 T4913 relcl correspond,aa
R1221 T4919 T4917 pobj which,of
R1222 T4920 T4921 det the,aa
R1223 T4921 T4918 nsubj aa,correspond
R1224 T4922 T4921 amod first,aa
R1225 T4923 T4921 nummod 580,aa
R1226 T4924 T4918 prep to,correspond
R1227 T4925 T4926 det the,protein
R1228 T4926 T4924 pobj protein,to
R1229 T4927 T4926 amod normal,protein
R1230 T4928 T4926 compound Apc,protein
R1231 T4929 T4877 punct .,results
R1232 T4931 T4932 compound Southern,blot
R1233 T4932 T4933 compound blot,analysis
R1234 T4933 T4934 nsubj analysis,confirmed
R1235 T4935 T4933 prep of,analysis
R1236 T4936 T4937 compound tail,DNA
R1237 T4937 T4935 pobj DNA,of
R1238 T4938 T4937 prep from,DNA
R1239 T4939 T4940 compound F1,offspring
R1240 T4940 T4938 pobj offspring,from
R1241 T4941 T4940 prep of,offspring
R1242 T4942 T4943 preconj both,ApcCKON
R1243 T4943 T4944 nmod ApcCKON,lines
R1244 T4944 T4941 pobj lines,of
R1245 T4945 T4943 cc and,ApcCKON
R1246 T4946 T4943 conj ApcΔ580,ApcCKON
R1247 T4947 T4948 det the,transmission
R1248 T4948 T4934 dobj transmission,confirmed
R1249 T4949 T4948 compound germline,transmission
R1250 T4950 T4948 prep of,transmission
R1251 T4951 T4952 amod modified,allele
R1252 T4952 T4950 pobj allele,of
R1253 T4953 T4952 compound Apc,allele
R1254 T4954 T4955 punct (,1B
R1255 T4955 T4934 parataxis 1B,confirmed
R1256 T4956 T4955 compound Figure,1B
R1257 T4957 T4955 punct ),1B
R1258 T4958 T4934 punct .,confirmed
R1259 T4960 T4961 nsubj Mice,are
R1260 T4962 T4963 dep that,are
R1261 T4963 T4960 relcl are,Mice
R1262 T4964 T4963 acomp heterozygous,are
R1263 T4965 T4964 prep for,heterozygous
R1264 T4966 T4967 compound ApcΔ580,mutation
R1265 T4967 T4965 pobj mutation,for
R1266 T4968 T4961 acomp viable,are
R1267 T4969 T4961 cc but,are
R1268 T4970 T4961 conj have,are
R1269 T4971 T4972 det a,lifespan
R1270 T4972 T4970 dobj lifespan,have
R1271 T4973 T4974 advmod significantly,reduced
R1272 T4974 T4972 amod reduced,lifespan
R1273 T4975 T4976 punct (,1C
R1274 T4976 T4970 parataxis 1C,have
R1275 T4977 T4976 compound Figure,1C
R1276 T4978 T4976 punct ),1C
R1277 T4979 T4961 punct .,are
R1278 T4981 T4982 det These,results
R1279 T4982 T4983 nsubj results,suggested
R1280 T4984 T4985 mark that,results
R1281 T4985 T4983 ccomp results,suggested
R1282 T4986 T4985 nsubj deletion,results
R1283 T4987 T4986 prep of,deletion
R1284 T4988 T4987 pobj exon,of
R1285 T4989 T4988 nummod 14,exon
R1286 T4990 T4985 advmod indeed,results
R1287 T4991 T4985 prep in,results
R1288 T4992 T4993 preconj either,loss
R1289 T4993 T4991 pobj loss,in
R1290 T4994 T4993 cc or,loss
R1291 T4995 T4996 amod abnormal,function
R1292 T4996 T4993 conj function,loss
R1293 T4997 T4993 prep of,loss
R1294 T4998 T4999 det the,product
R1295 T4999 T4997 pobj product,of
R1296 T5000 T5001 compound Apc,gene
R1297 T5001 T4999 compound gene,product
R1298 T5002 T4983 punct .,suggested
R1299 T5004 T5005 nmod ApcΔ580,mice
R1300 T5005 T5008 nsubj mice,have
R1301 T5006 T5004 punct /,ApcΔ580
R1302 T5007 T5004 punct +,ApcΔ580
R1303 T5009 T5010 amod median,survival
R1304 T5010 T5008 dobj survival,have
R1305 T5011 T5010 prep of,survival
R1306 T5012 T5013 nummod 5,mo
R1307 T5013 T5011 pobj mo,of
R1308 T5014 T5013 prep of,mo
R1309 T5015 T5014 pobj age,of
R1310 T5016 T5017 punct (,1C
R1311 T5017 T5010 parataxis 1C,survival
R1312 T5018 T5017 compound Figure,1C
R1313 T5019 T5017 punct ),1C
R1314 T5020 T5010 punct ", ",survival
R1315 T5021 T5010 prep with,survival
R1316 T5022 T5023 amod progressive,signs
R1317 T5023 T5021 pobj signs,with
R1318 T5024 T5023 prep of,signs
R1319 T5025 T5026 amod rectal,bleeding
R1320 T5026 T5024 pobj bleeding,of
R1321 T5131 T5129 nsubj mechanism,is
R1322 T5027 T5026 cc and,bleeding
R1323 T5028 T5026 conj anemia,bleeding
R1324 T5132 T5133 advmod most,likely
R1325 T5029 T5008 punct .,have
R1326 T5133 T5131 amod likely,mechanism
R1327 T5031 T5032 advcl Similar,had
R1328 T5033 T5031 prep to,Similar
R1329 T5134 T5131 prep of,mechanism
R1330 T5034 T5035 det the,results
R1331 T5035 T5033 pobj results,to
R1332 T5135 T5136 amod wild,type
R1333 T5036 T5035 acl reported,results
R1334 T5037 T5036 prep with,reported
R1335 T5038 T5039 det an,strain
R1336 T5136 T5138 compound type,inactivation
R1337 T5039 T5037 pobj strain,with
R1338 T5040 T5039 advmod independently,strain
R1339 T5041 T5039 amod generated,strain
R1340 T5137 T5136 punct -,type
R1341 T5042 T5043 npadvmod ApcΔ580,conditional
R1342 T5043 T5039 amod conditional,strain
R1343 T5044 T5042 punct /,ApcΔ580
R1344 T5138 T5134 pobj inactivation,of
R1345 T5045 T5042 punct +,ApcΔ580
R1346 T5046 T5039 compound mouse,strain
R1347 T5047 T5048 punct [,14
R1348 T5139 T5138 compound Apc,inactivation
R1349 T5048 T5036 parataxis 14,reported
R1350 T5049 T5048 punct ],14
R1351 T5050 T5032 punct ", ",had
R1352 T5140 T5129 prep by,is
R1353 T5051 T5052 nmod ApcΔ580,mice
R1354 T5052 T5032 nsubj mice,had
R1355 T5053 T5051 punct /,ApcΔ580
R1356 T5141 T5142 amod allelic,loss
R1357 T5054 T5051 punct +,ApcΔ580
R1358 T5055 T5056 amod more,100
R1359 T5056 T5058 nummod 100,tumors
R1360 T5142 T5140 pobj loss,by
R1361 T5057 T5056 quantmod than,100
R1362 T5143 T5116 punct .,showed
R1363 T5058 T5032 dobj tumors,had
R1364 T5059 T5060 punct (,37
R1365 T5060 T5056 parataxis 37,100
R1366 T5061 T5060 nummod 120,37
R1367 T5062 T5060 punct ±,37
R1368 T5145 T5146 det The,allele
R1369 T5063 T5060 punct ", ",37
R1370 T5064 T5065 nsubj n,11
R1371 T5065 T5060 ccomp 11,37
R1372 T5066 T5065 punct =,11
R1373 T5067 T5060 punct ),37
R1374 T5068 T5058 amod intestinal,tumors
R1375 T5069 T5032 prep at,had
R1376 T5070 T5071 det the,time
R1377 T5146 T5148 nsubj allele,had
R1378 T5071 T5069 pobj time,at
R1379 T5072 T5071 prep of,time
R1380 T5073 T5074 poss their,death
R1381 T5074 T5072 pobj death,of
R1382 T5147 T5146 amod mutant,allele
R1383 T5075 T5076 punct (,S1
R1384 T5076 T5032 parataxis S1,had
R1385 T5077 T5076 compound Figure,S1
R1386 T5149 T5150 aux to,maintained
R1387 T5078 T5076 punct ),S1
R1388 T5079 T5032 punct .,had
R1389 T5081 T5082 nsubj Inactivation,is
R1390 T5150 T5148 xcomp maintained,had
R1391 T5083 T5081 prep of,Inactivation
R1392 T5084 T5085 amod wild,type
R1393 T5151 T5150 auxpass be,maintained
R1394 T5085 T5087 compound type,Apc
R1395 T5086 T5085 punct -,type
R1396 T5087 T5083 pobj Apc,of
R1397 T5152 T5150 cc and,maintained
R1398 T5088 T5089 det an,prerequisite
R1399 T5089 T5082 attr prerequisite,is
R1400 T5090 T5089 amod important,prerequisite
R1401 T5153 T5150 conj transmitted,maintained
R1402 T5091 T5089 prep for,prerequisite
R1403 T5092 T5093 compound tumor,development
R1404 T5093 T5091 pobj development,for
R1405 T5154 T5153 prep through,transmitted
R1406 T5094 T5082 punct .,is
R1407 T5155 T5156 amod male,mice
R1408 T5096 T5097 nsubj We,analyzed
R1409 T5098 T5099 nummod 30,tumors
R1410 T5156 T5154 pobj mice,through
R1411 T5099 T5097 dobj tumors,analyzed
R1412 T5100 T5099 amod intestinal,tumors
R1413 T5157 T5153 punct ", ",transmitted
R1414 T5101 T5099 prep from,tumors
R1415 T5102 T5103 nmod ApcΔ580,mice
R1416 T5103 T5101 pobj mice,from
R1417 T5104 T5102 punct /,ApcΔ580
R1418 T5158 T5159 mark as,were
R1419 T5105 T5102 punct +,ApcΔ580
R1420 T5106 T5097 prep by,analyzed
R1421 T5159 T5153 advcl were,transmitted
R1422 T5160 T5161 nmod ApcΔ580,females
R1423 T5107 T5108 advmod in,vitro
R1424 T5108 T5109 amod vitro,transcription
R1425 T5109 T5106 pobj transcription,by
R1426 T5110 T5109 cc and,transcription
R1427 T5111 T5112 compound translation,assay
R1428 T5112 T5109 conj assay,transcription
R1429 T5161 T5159 nsubj females,were
R1430 T5113 T5097 punct ", ",analyzed
R1431 T5114 T5097 cc but,analyzed
R1432 T5115 T5116 nsubj none,showed
R1433 T5162 T5160 punct /,ApcΔ580
R1434 T5116 T5097 conj showed,analyzed
R1435 T5117 T5115 prep of,none
R1436 T5163 T5160 punct +,ApcΔ580
R1437 T5118 T5117 pobj them,of
R1438 T5119 T5120 amod truncated,products
R1439 T5120 T5116 dobj products,showed
R1440 T5164 T5159 advmod frequently,were
R1441 T5121 T5120 compound Apc,products
R1442 T5122 T5123 punct (,data
R1443 T5123 T5116 meta data,showed
R1444 T5165 T5159 neg not,were
R1445 T5124 T5123 amod unpublished,data
R1446 T5125 T5116 punct ),showed
R1447 T5126 T5116 punct ", ",showed
R1448 T5166 T5159 acomp healthy,were
R1449 T5127 T5116 advcl indicating,showed
R1450 T5128 T5129 mark that,is
R1451 T5129 T5127 ccomp is,indicating
R1452 T5167 T5166 advmod enough,healthy
R1453 T5130 T5131 det the,mechanism
R1454 T5168 T5169 aux to,nurse
R1455 T5169 T5166 xcomp nurse,healthy
R1456 T5170 T5169 advmod successfully,nurse
R1457 T5171 T5172 poss their,pups
R1458 T5237 T5238 amod wild,type
R1459 T5172 T5169 dobj pups,nurse
R1460 T5238 T5236 compound type,littermates
R1461 T5239 T5238 punct -,type
R1462 T5173 T5172 amod own,pups
R1463 T5240 T5241 punct (,1C
R1464 T5241 T5228 parataxis 1C,showing
R1465 T5242 T5241 compound Figure,1C
R1466 T5174 T5159 prep because,were
R1467 T5243 T5241 punct ),1C
R1468 T5244 T5217 punct .,are
R1469 T5175 T5174 pcomp of,because
R1470 T5246 T5247 nsubj We,tested
R1471 T5176 T5177 poss their,burden
R1472 T5248 T5249 mark whether,compliment
R1473 T5249 T5247 ccomp compliment,tested
R1474 T5177 T5174 pobj burden,because
R1475 T5250 T5251 poss our,allele
R1476 T5251 T5249 nsubj allele,compliment
R1477 T5252 T5251 compound ApcCKO,allele
R1478 T5253 T5249 aux can,compliment
R1479 T5254 T5255 det the,allele
R1480 T5255 T5249 dobj allele,compliment
R1481 T5256 T5257 amod wild,type
R1482 T5178 T5177 compound tumor,burden
R1483 T5257 T5255 compound type,allele
R1484 T5258 T5257 punct -,type
R1485 T5259 T5249 prep by,compliment
R1486 T5179 T5148 punct .,had
R1487 T5260 T5259 pcomp crossing,by
R1488 T5261 T5262 det the,female
R1489 T5262 T5260 dobj female,crossing
R1490 T5181 T5182 nmod ApcCKO,mice
R1491 T5263 T5264 compound ApcCKO,CKO
R1492 T5264 T5262 compound CKO,female
R1493 T5265 T5264 punct /,CKO
R1494 T5266 T5260 prep with,crossing
R1495 T5267 T5268 nmod ApcΔ580,mouse
R1496 T5268 T5266 pobj mouse,with
R1497 T5182 T5185 nsubjpass mice,intercrossed
R1498 T5269 T5267 punct /,ApcΔ580
R1499 T5270 T5267 punct +,ApcΔ580
R1500 T5271 T5268 amod male,mouse
R1501 T5183 T5181 punct /,ApcCKO
R1502 T5272 T5247 punct .,tested
R1503 T5184 T5181 punct +,ApcCKO
R1504 T5274 T5275 det The,offspring
R1505 T5275 T5280 nsubj offspring,were
R1506 T5276 T5275 amod resultant,offspring
R1507 T5186 T5185 auxpass were,intercrossed
R1508 T5277 T5278 compound ApcCKO,Δ580
R1509 T5278 T5275 compound Δ580,offspring
R1510 T5279 T5278 punct /,Δ580
R1511 T5187 T5188 aux to,generate
R1512 T5188 T5185 advcl generate,intercrossed
R1513 T5189 T5190 compound ApcCKO,CKO
R1514 T5281 T5280 acomp viable,were
R1515 T5190 T5192 compound CKO,offspring
R1516 T5191 T5190 punct /,CKO
R1517 T5282 T5281 cc and,viable
R1518 T5283 T5281 conj born,viable
R1519 T5192 T5188 dobj offspring,generate
R1520 T5284 T5283 prep in,born
R1521 T5285 T5286 det the,ratio
R1522 T5286 T5284 pobj ratio,in
R1523 T5193 T5185 punct .,intercrossed
R1524 T5287 T5286 amod Mendelian,ratio
R1525 T5288 T5280 punct ", ",were
R1526 T5289 T5280 advcl suggesting,were
R1527 T5195 T5196 advmod Approximately,one
R1528 T5290 T5291 mark that,have
R1529 T5291 T5289 ccomp have,suggesting
R1530 T5292 T5293 det the,presence
R1531 T5293 T5291 nsubj presence,have
R1532 T5294 T5293 prep of,presence
R1533 T5196 T5197 nsubj one,were
R1534 T5295 T5296 compound loxP,sites
R1535 T5296 T5294 pobj sites,of
R1536 T5297 T5293 prep in,presence
R1537 T5198 T5196 punct -,one
R1538 T5298 T5297 pobj introns,in
R1539 T5299 T5298 acl flanking,introns
R1540 T5300 T5299 dobj exon,flanking
R1541 T5301 T5300 nummod 14,exon
R1542 T5199 T5196 amod quarter,one
R1543 T5302 T5303 det no,effect
R1544 T5200 T5196 prep of,one
R1545 T5201 T5202 det the,offspring
R1546 T5202 T5200 pobj offspring,of
R1547 T5303 T5291 dobj effect,have
R1548 T5203 T5204 punct (,17
R1549 T5304 T5303 amod adverse,effect
R1550 T5305 T5303 prep on,effect
R1551 T5204 T5196 parataxis 17,one
R1552 T5306 T5307 det the,function
R1553 T5307 T5305 pobj function,on
R1554 T5308 T5307 prep of,function
R1555 T5205 T5204 prep of,17
R1556 T5309 T5310 det the,gene
R1557 T5310 T5308 pobj gene,of
R1558 T5311 T5310 compound Apc,gene
R1559 T5206 T5205 pobj 81,of
R1560 T5312 T5280 punct .,were
R1561 T5207 T5204 punct ),17
R1562 T5208 T5197 acomp homozygous,were
R1563 T5209 T5208 prep for,homozygous
R1564 T5210 T5211 det the,allele
R1565 T5211 T5209 pobj allele,for
R1566 T5212 T5211 compound ApcCKO,allele
R1567 T5213 T5197 punct .,were
R1568 T5215 T5216 det These,mice
R1569 T5216 T5217 nsubj mice,are
R1570 T5218 T5219 advmod as,as
R1571 T5219 T5216 cc as,mice
R1572 T5220 T5219 advmod well,as
R1573 T5221 T5222 amod heterozygous,mice
R1574 T5222 T5216 conj mice,mice
R1575 T5223 T5222 prep for,mice
R1576 T5224 T5225 compound ApcCKO,allele
R1577 T5225 T5223 pobj allele,for
R1578 T5226 T5217 acomp normal,are
R1579 T5227 T5217 punct ", ",are
R1580 T5228 T5217 advcl showing,are
R1581 T5229 T5230 det no,differences
R1582 T5230 T5228 dobj differences,showing
R1583 T5231 T5230 prep in,differences
R1584 T5232 T5233 poss their,survival
R1585 T5233 T5231 pobj survival,in
R1586 T5234 T5230 prep to,differences
R1587 T5235 T5236 det the,littermates
R1588 T5236 T5234 pobj littermates,to
R1595 T7674 T7675 npadvmod K14,Driven
R1596 T7675 T7677 amod Driven,Loss
R1597 T7676 T7675 punct -,Driven
R1598 T7677 T7678 nsubj Loss,Results
R1599 T7679 T7677 prep of,Loss
R1600 T7680 T7679 pobj Apc,of
R1601 T7681 T7678 prep in,Results
R1602 T7682 T7683 amod Severe,Retardation
R1603 T7683 T7681 pobj Retardation,in
R1604 T7684 T7683 compound Growth,Retardation
R1605 T7685 T7683 cc and,Retardation
R1606 T7686 T7687 amod Early,Lethality
R1607 T7687 T7683 conj Lethality,Retardation
R1608 T7689 T7690 aux To,introduce
R1609 T7690 T7691 advcl introduce,crossed
R1610 T7692 T7693 det the,mutation
R1611 T7693 T7690 dobj mutation,introduce
R1612 T7694 T7693 prep of,mutation
R1613 T7695 T7694 pobj Apc,of
R1614 T7696 T7690 prep into,introduce
R1615 T7697 T7696 pobj cells,into
R1616 T7698 T7697 acl expressing,cells
R1617 T7699 T7698 dobj K14,expressing
R1618 T7700 T7691 punct ", ",crossed
R1619 T7701 T7691 nsubj we,crossed
R1620 T7702 T7703 nmod WW6,mice
R1621 T7703 T7691 dobj mice,crossed
R1622 T7704 T7705 compound ES,cell
R1623 T7705 T7706 npadvmod cell,derived
R1624 T7706 T7703 amod derived,mice
R1625 T7707 T7706 punct –,derived
R1626 T7708 T7709 punct [,15
R1627 T7709 T7706 parataxis 15,derived
R1628 T7710 T7709 punct ],15
R1629 T7711 T7703 nmod ApcCKO,mice
R1630 T7712 T7711 punct /,ApcCKO
R1631 T7713 T7711 punct +,ApcCKO
R1632 T7714 T7703 prep with,mice
R1633 T7715 T7716 compound K14,cre
R1634 T7716 T7718 compound cre,mice
R1635 T7717 T7716 punct -,cre
R1636 T7718 T7714 pobj mice,with
R1637 T7719 T7718 compound recombinase,mice
R1638 T7720 T7703 prep in,mice
R1639 T7721 T7722 compound FVB,background
R1640 T7722 T7720 pobj background,in
R1641 T7723 T7724 punct [,16
R1642 T7724 T7691 parataxis 16,crossed
R1643 T7725 T7724 punct ],16
R1644 T7726 T7691 punct .,crossed
R1645 T7728 T7729 det The,mice
R1646 T7729 T7737 nsubj mice,were
R1647 T7730 T7731 nmod K14,cre
R1648 T7731 T7729 nmod cre,mice
R1649 T7732 T7731 punct -,cre
R1650 T7733 T7731 punct ;,cre
R1651 T7734 T7731 appos ApcCKO,cre
R1652 T7735 T7734 punct /,ApcCKO
R1653 T7736 T7734 punct +,ApcCKO
R1654 T7738 T7737 acomp normal,were
R1655 T7739 T7737 prep in,were
R1656 T7740 T7739 pobj appearance,in
R1657 T7741 T7737 cc and,were
R1658 T7742 T7737 conj were,were
R1659 T7743 T7742 acomp fertile,were
R1660 T7744 T7737 punct .,were
R1661 T7746 T7747 nmod K14,cre
R1662 T7747 T7749 nmod cre,males
R1663 T7748 T7747 punct -,cre
R1664 T7749 T7754 nsubjpass males,crossed
R1665 T7750 T7747 punct ;,cre
R1666 T7751 T7747 appos ApcCKO,cre
R1667 T7752 T7751 punct /,ApcCKO
R1668 T7753 T7751 punct +,ApcCKO
R1669 T7755 T7754 auxpass were,crossed
R1670 T7756 T7754 prep to,crossed
R1671 T7757 T7758 compound ApcCKO,CKO
R1672 T7758 T7760 compound CKO,females
R1673 T7759 T7758 punct /,CKO
R1674 T7760 T7756 pobj females,to
R1675 T7761 T7762 aux to,avoid
R1676 T7762 T7754 advcl avoid,crossed
R1677 T7763 T7764 det the,effect
R1678 T7764 T7762 dobj effect,avoid
R1679 T7765 T7764 amod potential,effect
R1680 T7766 T7764 compound deleter,effect
R1681 T7767 T7762 prep in,avoid
R1682 T7768 T7767 pobj oocytes,in
R1683 T7769 T7768 prep of,oocytes
R1684 T7770 T7771 compound K14,cre
R1685 T7771 T7773 npadvmod cre,positive
R1686 T7772 T7771 punct -,cre
R1687 T7773 T7775 amod positive,females
R1688 T7774 T7773 punct –,positive
R1689 T7775 T7769 pobj females,of
R1690 T7776 T7777 punct [,17
R1691 T7777 T7754 parataxis 17,crossed
R1692 T7778 T7777 punct ],17
R1693 T7779 T7754 punct .,crossed
R1694 T7781 T7782 det The,mice
R1695 T7782 T7783 nsubjpass mice,intercrossed
R1696 T7783 T7785 ccomp intercrossed,were
R1697 T7784 T7783 auxpass were,intercrossed
R1698 T7786 T7783 advmod thereafter,intercrossed
R1699 T7787 T7783 prep for,intercrossed
R1700 T7788 T7787 pobj maintenance,for
R1701 T7789 T7785 punct ;,were
R1702 T7790 T7785 advmod hence,were
R1703 T7791 T7785 punct ", ",were
R1704 T7792 T7793 det the,mice
R1705 T7793 T7785 nsubj mice,were
R1706 T7794 T7793 acl used,mice
R1707 T7795 T7794 prep for,used
R1708 T7796 T7795 pobj analysis,for
R1709 T7797 T7785 prep in,were
R1710 T7798 T7799 det a,background
R1711 T7799 T7797 pobj background,in
R1712 T7800 T7799 amod mixed,background
R1713 T7801 T7799 prep of,background
R1714 T7802 T7801 pobj FVB,of
R1715 T7803 T7802 punct ", ",FVB
R1716 T7804 T7805 nummod 129,S
R1717 T7805 T7802 conj S,FVB
R1718 T7806 T7805 punct /,S
R1719 T7807 T7805 punct ", ",S
R1720 T7808 T7805 cc and,S
R1721 T7809 T7805 conj C57BL,S
R1722 T7810 T7809 punct /,C57BL
R1723 T7811 T7809 nummod 6,C57BL
R1724 T7812 T7802 prep in,FVB
R1725 T7813 T7814 amod similar,proportions
R1726 T7814 T7812 pobj proportions,in
R1727 T7815 T7802 punct ", ",FVB
R1728 T7816 T7802 prep with,FVB
R1729 T7817 T7818 amod minimal,contribution
R1730 T7818 T7816 pobj contribution,with
R1731 T7819 T7818 prep of,contribution
R1732 T7820 T7819 pobj SJ,of
R1733 T7821 T7785 punct .,were
R1734 T7824 T7826 nsubj promoter,is
R1735 T7823 T7824 det The,promoter
R1736 T7825 T7824 compound K14,promoter
R1737 T7929 T7928 pobj crosses,from
R1738 T7827 T7828 det a,promoter
R1739 T7930 T7929 prep between,crosses
R1740 T7828 T7826 attr promoter,is
R1741 T7829 T7830 advmod commonly,used
R1742 T7830 T7828 amod used,promoter
R1743 T7831 T7832 amod epidermal,cell
R1744 T7832 T7828 compound cell,promoter
R1745 T7833 T7826 prep because,is
R1746 T7931 T7932 nmod K14,cre
R1747 T7834 T7833 pcomp of,because
R1748 T7835 T7836 poss its,expression
R1749 T7836 T7833 pobj expression,because
R1750 T7932 T7934 nmod cre,mice
R1751 T7837 T7836 prep by,expression
R1752 T7838 T7839 det the,cells
R1753 T7839 T7837 pobj cells,by
R1754 T7933 T7932 punct -,cre
R1755 T7840 T7841 advmod mitotically,active
R1756 T7841 T7839 amod active,cells
R1757 T7842 T7839 prep of,cells
R1758 T7934 T7930 pobj mice,between
R1759 T7843 T7844 det the,epidermis
R1760 T7844 T7842 pobj epidermis,of
R1761 T7845 T7844 cc and,epidermis
R1762 T7846 T7847 poss its,appendages
R1763 T7847 T7844 conj appendages,epidermis
R1764 T7848 T7836 prep in,expression
R1765 T7935 T7932 punct ;,cre
R1766 T7849 T7850 amod mature,skin
R1767 T7850 T7848 pobj skin,in
R1768 T7851 T7852 punct [,18
R1769 T7936 T7932 appos ApcCKO,cre
R1770 T7852 T7836 parataxis 18,expression
R1771 T7853 T7852 punct ],18
R1772 T7937 T7936 punct /,ApcCKO
R1773 T7938 T7936 punct +,ApcCKO
R1774 T7854 T7826 punct ", ",is
R1775 T7939 T7932 cc and,cre
R1776 T7855 T7826 cc but,is
R1777 T7856 T7857 advmod most,notably
R1778 T7857 T7858 advmod notably,is
R1779 T7858 T7826 conj is,is
R1780 T7940 T7941 compound ApcCKO,CKO
R1781 T7859 T7858 nsubj it,is
R1782 T7860 T7858 acomp active,is
R1783 T7941 T7932 conj CKO,cre
R1784 T7861 T7858 prep in,is
R1785 T7862 T7863 amod embryonic,ectoderm
R1786 T7942 T7941 punct /,CKO
R1787 T7863 T7861 pobj ectoderm,in
R1788 T7864 T7865 advmod as,early
R1789 T7865 T7858 advmod early,is
R1790 T7943 T7911 punct .,genotyped
R1791 T7866 T7865 prep as,early
R1792 T7867 T7868 det the,stage
R1793 T7868 T7866 pobj stage,as
R1794 T7945 T7946 det The,mice
R1795 T7869 T7870 amod single,layered
R1796 T7870 T7868 amod layered,stage
R1797 T7871 T7868 amod ectodermal,stage
R1798 T7872 T7868 prep of,stage
R1799 T7873 T7874 amod embryonic,day
R1800 T7874 T7872 pobj day,of
R1801 T7875 T7876 punct (,E
R1802 T7946 T7948 nsubjpass mice,born
R1803 T7876 T7874 parataxis E,day
R1804 T7877 T7876 punct ),E
R1805 T7878 T7874 nummod 9.5,day
R1806 T7879 T7880 punct [,19
R1807 T7947 T7946 amod mutant,mice
R1808 T7880 T7858 parataxis 19,is
R1809 T7881 T7880 punct ],19
R1810 T7949 T7946 prep of,mice
R1811 T7882 T7858 punct .,is
R1812 T7884 T7885 det A,expression
R1813 T7885 T7887 nsubjpass expression,found
R1814 T7886 T7885 amod restricted,expression
R1815 T7950 T7951 det the,genotype
R1816 T7888 T7885 prep of,expression
R1817 T7889 T7888 pobj K14,of
R1818 T7890 T7887 auxpass is,found
R1819 T7951 T7949 pobj genotype,of
R1820 T7891 T7887 advmod also,found
R1821 T7892 T7887 prep in,found
R1822 T7893 T7894 amod thymic,cells
R1823 T7952 T7953 compound K14,cre
R1824 T7894 T7892 pobj cells,in
R1825 T7895 T7894 amod epithelial,cells
R1826 T7953 T7951 appos cre,genotype
R1827 T7896 T7894 punct (,cells
R1828 T7897 T7894 appos TECs,cells
R1829 T7898 T7887 punct ),found
R1830 T7954 T7953 punct -,cre
R1831 T7899 T7887 prep in,found
R1832 T7900 T7901 det the,medulla
R1833 T7901 T7899 pobj medulla,in
R1834 T7902 T7901 prep of,medulla
R1835 T7955 T7953 punct ;,cre
R1836 T7903 T7904 amod normal,thymus
R1837 T7904 T7902 pobj thymus,of
R1838 T7905 T7906 punct [,20
R1839 T7956 T7957 compound ApcCKO,CKO
R1840 T7906 T7887 parataxis 20,found
R1841 T7907 T7906 punct ],20
R1842 T7908 T7887 punct .,found
R1843 T7957 T7953 appos CKO,cre
R1844 T7910 T7911 nsubj We,genotyped
R1845 T7958 T7957 punct /,CKO
R1846 T7912 T7913 quantmod a,458
R1847 T7913 T7916 nummod 458,pups
R1848 T7914 T7913 quantmod total,458
R1849 T7915 T7913 quantmod of,458
R1850 T7916 T7911 dobj pups,genotyped
R1851 T7959 T7960 punct (,mutant
R1852 T7917 T7918 punct (,old
R1853 T7918 T7916 parataxis old,pups
R1854 T7919 T7920 quantmod 8,10
R1855 T7920 T7922 nummod 10,d
R1856 T7960 T7946 parataxis mutant,mice
R1857 T7921 T7920 punct –,10
R1858 T7922 T7918 npadvmod d,old
R1859 T7923 T7918 punct ),old
R1860 T7961 T7960 advmod hereafter,mutant
R1861 T7924 T7916 prep from,pups
R1862 T7925 T7926 nummod 67,litters
R1863 T7926 T7924 pobj litters,from
R1864 T7962 T7960 punct ", ",mutant
R1865 T7927 T7926 acl resulting,litters
R1866 T7928 T7927 prep from,resulting
R1867 T7963 T7960 compound KA,mutant
R1868 T7964 T7960 punct ),mutant
R1869 T7965 T7948 auxpass were,born
R1870 T7966 T7948 punct ", ",born
R1871 T7967 T7948 cc but,born
R1872 T7968 T7969 det the,frequency
R1873 T8034 T8037 nummod 25,pups
R1874 T7969 T7971 nsubj frequency,was
R1875 T8035 T8034 quantmod total,25
R1876 T8036 T8034 quantmod of,25
R1877 T7970 T7969 amod observed,frequency
R1878 T8037 T8038 nsubjpass pups,born
R1879 T8039 T8038 auxpass were,born
R1880 T7971 T7948 conj was,born
R1881 T8040 T8038 prep from,born
R1882 T8041 T8042 nummod three,litters
R1883 T7972 T7969 prep of,frequency
R1884 T8042 T8040 pobj litters,from
R1885 T8043 T8042 punct ", ",litters
R1886 T8044 T8045 prep of,confirmed
R1887 T7973 T7974 compound KA,mutants
R1888 T8045 T8042 relcl confirmed,litters
R1889 T8046 T8044 pobj which,of
R1890 T8047 T8045 nsubjpass 7,confirmed
R1891 T7974 T7972 pobj mutants,of
R1892 T8048 T8049 punct (,%
R1893 T8049 T8047 parataxis %,7
R1894 T7975 T7976 advmod much,less
R1895 T8050 T8049 nummod 28,%
R1896 T8051 T8049 punct ),%
R1897 T8052 T8045 auxpass were,confirmed
R1898 T8053 T8054 aux to,be
R1899 T8054 T8045 xcomp be,confirmed
R1900 T8055 T8056 compound K14,cre
R1901 T8056 T8054 attr cre,be
R1902 T7976 T7971 acomp less,was
R1903 T8057 T8056 punct -,cre
R1904 T8058 T8056 punct ;,cre
R1905 T8059 T8060 compound ApcCKO,CKO
R1906 T7977 T7978 mark than,expected
R1907 T8060 T8056 appos CKO,cre
R1908 T8061 T8060 punct /,CKO
R1909 T8062 T8045 prep by,confirmed
R1910 T7978 T7976 advcl expected,less
R1911 T8063 T8062 pobj genotyping,by
R1912 T8064 T8045 punct ", ",confirmed
R1913 T7979 T7980 punct (,Table
R1914 T8065 T8045 advcl indicating,confirmed
R1915 T8066 T8067 mark that,born
R1916 T8067 T8065 ccomp born,indicating
R1917 T7980 T7976 parataxis Table,less
R1918 T8068 T8069 compound KA,mutants
R1919 T8069 T8067 nsubjpass mutants,born
R1920 T8070 T8067 auxpass were,born
R1921 T7981 T7980 dep 78,Table
R1922 T8071 T8067 prep in,born
R1923 T8072 T8073 det the,ratio
R1924 T8073 T8071 pobj ratio,in
R1925 T7982 T7981 prep of,78
R1926 T8074 T8073 amod expected,ratio
R1927 T8075 T8073 amod Mendelian,ratio
R1928 T7983 T7982 pobj 458,of
R1929 T8076 T8038 punct .,born
R1930 T8078 T8079 det The,pups
R1931 T7984 T7985 punct [,%
R1932 T8079 T8082 nsubjpass pups,nursed
R1933 T8080 T8079 nmod KA,pups
R1934 T8081 T8079 amod mutant,pups
R1935 T7985 T7981 parataxis %,78
R1936 T8083 T8082 auxpass were,nursed
R1937 T7986 T7985 nummod 17.0,%
R1938 T8084 T8082 advmod normally,nursed
R1939 T8085 T8082 punct ", ",nursed
R1940 T8086 T8082 cc and,nursed
R1941 T7987 T7985 punct ],%
R1942 T8087 T8088 expl there,was
R1943 T8088 T8082 conj was,nursed
R1944 T8089 T8088 attr milk,was
R1945 T7988 T7980 punct ;,Table
R1946 T8090 T8088 prep in,was
R1947 T8091 T8092 poss their,stomachs
R1948 T7989 T7990 nsubj p,analysis
R1949 T8092 T8090 pobj stomachs,in
R1950 T8093 T8088 prep during,was
R1951 T8094 T8095 det the,d
R1952 T8095 T8093 pobj d,during
R1953 T8096 T8095 amod first,d
R1954 T8097 T8095 nummod 2,d
R1955 T8098 T8097 cc or,2
R1956 T7990 T7980 ccomp analysis,Table
R1957 T8099 T8097 conj 3,2
R1958 T8100 T8095 prep after,d
R1959 T8101 T8100 pobj birth,after
R1960 T7991 T7990 punct <,analysis
R1961 T8102 T8082 punct ", ",nursed
R1962 T8103 T8082 cc but,nursed
R1963 T8104 T8105 nsubj they,failed
R1964 T7992 T7990 nummod 0.0005,analysis
R1965 T8105 T8082 conj failed,nursed
R1966 T7993 T7994 compound Chi,square
R1967 T8106 T8107 aux to,thrive
R1968 T8107 T8105 xcomp thrive,failed
R1969 T7994 T7990 compound square,analysis
R1970 T8108 T8109 punct (,Figure
R1971 T8109 T8107 parataxis Figure,thrive
R1972 T8110 T8109 nummod 2,Figure
R1973 T7995 T7994 punct -,square
R1974 T8111 T8109 punct ),Figure
R1975 T8112 T8105 punct .,failed
R1976 T7996 T7980 punct ", ",Table
R1977 T8114 T8115 prep By,were
R1978 T7997 T7980 nummod 1,Table
R1979 T8116 T8117 amod postnatal,day
R1980 T8117 T8114 pobj day,By
R1981 T7998 T7980 punct ),Table
R1982 T8118 T8117 punct (,day
R1983 T8119 T8117 appos P,day
R1984 T7999 T7971 punct .,was
R1985 T8120 T8117 punct ),day
R1986 T8121 T8117 appos 8,day
R1987 T8122 T8123 punct –,10
R1988 T8001 T8002 aux To,assess
R1989 T8123 T8121 prep 10,8
R1990 T8124 T8115 punct ", ",were
R1991 T8125 T8115 prep at,were
R1992 T8002 T8003 advcl assess,monitored
R1993 T8126 T8127 det the,time
R1994 T8127 T8125 pobj time,at
R1995 T8128 T8127 prep of,time
R1996 T8129 T8128 pobj genotyping,of
R1997 T8004 T8005 det the,basis
R1998 T8130 T8115 punct ", ",were
R1999 T8131 T8132 amod many,pups
R2000 T8005 T8002 dobj basis,assess
R2001 T8132 T8115 nsubj pups,were
R2002 T8133 T8132 nmod KA,pups
R2003 T8134 T8132 amod mutant,pups
R2004 T8135 T8136 advmod considerably,smaller
R2005 T8006 T8005 prep for,basis
R2006 T8136 T8115 acomp smaller,were
R2007 T8137 T8136 prep than,smaller
R2008 T8138 T8139 poss their,littermates
R2009 T8007 T8008 det the,lethality
R2010 T8139 T8137 pobj littermates,than
R2011 T8008 T8006 pobj lethality,for
R2012 T8009 T8008 amod neonatal,lethality
R2013 T8010 T8008 prep of,lethality
R2014 T8140 T8141 punct (,2B
R2015 T8141 T8115 parataxis 2B,were
R2016 T8011 T8012 compound KA,mutants
R2017 T8142 T8141 compound Figure,2B
R2018 T8143 T8144 punct –,2F
R2019 T8144 T8141 prep 2F,2B
R2020 T8012 T8010 pobj mutants,of
R2021 T8145 T8141 punct ),2B
R2022 T8146 T8115 cc and,were
R2023 T8013 T8003 punct ", ",monitored
R2024 T8147 T8148 nsubj some,died
R2025 T8148 T8115 conj died,were
R2026 T8149 T8148 aux have,died
R2027 T8014 T8003 nsubj we,monitored
R2028 T8150 T8148 prep at,died
R2029 T8151 T8150 cc or,at
R2030 T8015 T8016 nummod three,litters
R2031 T8152 T8153 amod prior,to
R2032 T8153 T8150 conj to,at
R2033 T8154 T8155 det this,age
R2034 T8016 T8003 dobj litters,monitored
R2035 T8155 T8153 pobj age,to
R2036 T8156 T8115 punct .,were
R2037 T8017 T8003 prep from,monitored
R2038 T8158 T8159 nsubj None,survived
R2039 T8018 T8017 pobj birth,from
R2040 T8160 T8158 prep of,None
R2041 T8161 T8162 compound KA,mutants
R2042 T8162 T8160 pobj mutants,of
R2043 T8019 T8003 prep to,monitored
R2044 T8163 T8159 prep to,survived
R2045 T8164 T8165 amod weaning,age
R2046 T8165 T8163 pobj age,to
R2047 T8020 T8019 pobj weaning,to
R2048 T8166 T8159 punct .,survived
R2049 T8021 T8003 prep by,monitored
R2050 T8168 T8169 det "Table 1 Genotype Distribution of Progeny from the Matings Figure 2 Postnatal Mortality and Stunted Growth in K14-cre; ApcCKO/CKO Mutant Mice Animals whose genotype is either heterozygous or homozygous for the wild-type Apc allele are referred to as normal (N); those whose genotype are K14-cre; ApcCKO/CKO and show the presence of K14-cre–recombined mutant Apc allele are called mutant (M). (A) Two P3 mutant mice, M1 and M2, and their normal littermates, showing size variation among mutants. (B) P8 mutant mouse (right) and a normal littermate. Note sparseness of hair coat and abnormal ears. (C–D) Vibrissae of whisker pads are short and oddly angled in a P12 mutant mouse (C), relative to control (D). Note the lack of incisors in the mutant. (E) A P17 mutant mouse (right) with its littermate. Its bare forehead, dorsal median line, and abnormal ears are evident. (F) Growth curve of mutants and normal littermates. Mutants exhibit stunted growth, which became more prominent as they aged, and weigh significantly less than littermates from P8 (p < 0.05). (G) Comparison of mutant and normal thymus from P3 mice. The mutant thymus (left) is dramatically smaller for its age compared to the normal littermate (right). The scale bar equals 1 mm. (H) Skeletal preparations of normal (left) and mutant (right), showing differences in development of both incisor (I) and molar (M) teeth. The",ability
R2051 T8169 T8170 nsubjpass ability,used
R2052 T8022 T8021 pcomp measuring,by
R2053 T8171 T8169 prep of,ability
R2054 T8172 T8173 amod whole,embryos
R2055 T8173 T8171 pobj embryos,of
R2056 T8174 T8175 aux to,exclude
R2057 T8175 T8169 acl exclude,ability
R2058 T8176 T8177 amod blue,dye
R2059 T8023 T8024 det the,weight
R2060 T8177 T8175 dobj dye,exclude
R2061 T8178 T8170 auxpass was,used
R2062 T8179 T8180 aux to,examine
R2063 T8024 T8022 dobj weight,measuring
R2064 T8180 T8170 advcl examine,used
R2065 T8181 T8182 det the,barrier
R2066 T8182 T8180 dobj barrier,examine
R2067 T8183 T8182 amod epidermal,barrier
R2068 T8025 T8024 compound body,weight
R2069 T8184 T8182 punct ", ",barrier
R2070 T8185 T8186 advmod normally,acquired
R2071 T8186 T8182 acl acquired,barrier
R2072 T8187 T8186 advcl beginning,acquired
R2073 T8026 T8024 prep of,weight
R2074 T8188 T8187 prep at,beginning
R2075 T8189 T8188 pobj E16,at
R2076 T8190 T8187 cc and,beginning
R2077 T8027 T8028 det each,pup
R2078 T8191 T8187 conj complete,beginning
R2079 T8028 T8026 pobj pup,of
R2080 T8192 T8191 prep by,complete
R2081 T8029 T8030 det every,day
R2082 T8193 T8192 pobj E18.5,by
R2083 T8194 T8195 punct [,21
R2084 T8195 T8186 parataxis 21,acquired
R2085 T8030 T8022 npadvmod day,measuring
R2086 T8196 T8195 punct ],21
R2087 T8197 T8170 punct .,used
R2088 T8031 T8003 punct .,monitored
R2089 T8199 T8200 nsubj Analyses,showed
R2090 T8201 T8199 prep of,Analyses
R2091 T8033 T8034 quantmod A,25
R2092 T8202 T8203 nmod E17.5,mutants
R2093 T8203 T8201 pobj mutants,of
R2094 T8204 T8205 punct –,E18.5
R2095 T8246 T8230 punct ", ",were
R2096 T8205 T8202 prep E18.5,E17.5
R2097 T8206 T8203 compound KA,mutants
R2098 T8207 T8208 mark that,were
R2099 T8208 T8200 ccomp were,showed
R2100 T8209 T8208 nsubj they,were
R2101 T8247 T8230 cc but,were
R2102 T8210 T8208 acomp able,were
R2103 T8211 T8212 aux to,exclude
R2104 T8248 T8249 det the,mutants
R2105 T8212 T8210 xcomp exclude,able
R2106 T8213 T8214 amod blue,dye
R2107 T8214 T8212 dobj dye,exclude
R2108 T8215 T8200 punct ", ",showed
R2109 T8216 T8200 advcl indicating,showed
R2110 T8249 T8250 nsubj mutants,showed
R2111 T8217 T8218 mark that,was
R2112 T8218 T8216 ccomp was,indicating
R2113 T8219 T8220 det the,barrier
R2114 T8250 T8230 conj showed,were
R2115 T8220 T8218 nsubj barrier,was
R2116 T8221 T8220 amod epidermal,barrier
R2117 T8222 T8218 acomp intact,was
R2118 T8251 T8252 det a,patch
R2119 T8223 T8224 punct (,S2
R2120 T8224 T8218 parataxis S2,was
R2121 T8225 T8224 compound Figure,S2
R2122 T8252 T8250 dobj patch,showed
R2123 T8226 T8224 punct ),S2
R2124 T8227 T8200 punct .,showed
R2125 T8253 T8252 prep of,patch
R2126 T8229 T8230 prep At,were
R2127 T8254 T8255 punct “,birthmark
R2128 T8231 T8232 det these,ages
R2129 T8232 T8229 pobj ages,At
R2130 T8233 T8232 amod embryonic,ages
R2131 T8255 T8253 pobj birthmark,of
R2132 T8234 T8230 punct ", ",were
R2133 T8235 T8230 expl there,were
R2134 T8236 T8237 det no,differences
R2135 T8256 T8255 punct ”,birthmark
R2136 T8237 T8230 attr differences,were
R2137 T8238 T8237 prep in,differences
R2138 T8239 T8238 pobj size,in
R2139 T8257 T8255 cc or,birthmark
R2140 T8240 T8237 prep between,differences
R2141 T8241 T8242 det the,mutants
R2142 T8242 T8240 pobj mutants,between
R2143 T8258 T8259 amod dark,pigmentation
R2144 T8243 T8242 cc and,mutants
R2145 T8244 T8245 poss their,littermates
R2146 T8245 T8242 conj littermates,mutants
R2147 T8259 T8255 conj pigmentation,birthmark
R2148 T8260 T8252 prep on,patch
R2149 T8261 T8262 poss their,foreheads
R2150 T8262 T8260 pobj foreheads,on
R2151 T8352 T8353 advmod phenotypically,normal
R2152 T8263 T8252 cc and,patch
R2153 T8353 T8351 amod normal,littermates
R2154 T8354 T8355 det a,coat
R2155 T8355 T8349 dobj coat,had
R2156 T8264 T8265 det a,line
R2157 T8356 T8355 amod smooth,coat
R2158 T8357 T8355 amod thin,coat
R2159 T8358 T8355 prep of,coat
R2160 T8359 T8358 pobj hair,of
R2161 T8360 T8361 punct (,2B
R2162 T8265 T8252 conj line,patch
R2163 T8361 T8349 parataxis 2B,had
R2164 T8362 T8361 compound Figure,2B
R2165 T8363 T8361 punct ),2B
R2166 T8364 T8336 punct .,were
R2167 T8266 T8265 amod dark,line
R2168 T8267 T8265 amod median,line
R2169 T8366 T8367 prep At,start
R2170 T8268 T8269 dep that,ran
R2171 T8368 T8369 det this,age
R2172 T8369 T8366 pobj age,At
R2173 T8269 T8265 relcl ran,line
R2174 T8370 T8367 punct ", ",start
R2175 T8371 T8372 nummod two,incisors
R2176 T8372 T8367 nsubj incisors,start
R2177 T8270 T8269 advmod caudally,ran
R2178 T8373 T8372 amod lower,incisors
R2179 T8374 T8375 aux to,erupt
R2180 T8375 T8367 xcomp erupt,start
R2181 T8271 T8269 prep from,ran
R2182 T8376 T8375 prep in,erupt
R2183 T8377 T8378 amod normal,littermates
R2184 T8272 T8271 pobj head,from
R2185 T8378 T8376 pobj littermates,in
R2186 T8379 T8367 cc and,start
R2187 T8273 T8269 prep to,ran
R2188 T8380 T8381 nsubj these,were
R2189 T8381 T8367 conj were,start
R2190 T8382 T8381 acomp absent,were
R2191 T8274 T8273 pobj tail,to
R2192 T8383 T8381 prep in,were
R2193 T8384 T8385 det the,mutants
R2194 T8385 T8383 pobj mutants,in
R2195 T8275 T8250 punct .,showed
R2196 T8386 T8385 compound KA,mutants
R2197 T8387 T8388 punct (,2C
R2198 T8277 T8278 poss Their,ears
R2199 T8388 T8381 parataxis 2C,were
R2200 T8389 T8388 compound Figure,2C
R2201 T8390 T8388 cc and,2C
R2202 T8391 T8388 conj 2D,2C
R2203 T8392 T8388 punct ),2C
R2204 T8278 T8280 nsubj ears,were
R2205 T8393 T8381 punct .,were
R2206 T8395 T8396 nsubj Animals,tended
R2207 T8279 T8278 amod external,ears
R2208 T8397 T8396 advmod also,tended
R2209 T8281 T8278 cc or,ears
R2210 T8398 T8399 aux to,be
R2211 T8399 T8396 xcomp be,tended
R2212 T8400 T8399 acomp smaller,be
R2213 T8401 T8396 cc and,tended
R2214 T8402 T8403 prep around,displayed
R2215 T8403 T8396 conj displayed,tended
R2216 T8404 T8402 pobj P10,around
R2217 T8405 T8406 punct –,P12
R2218 T8282 T8278 conj pinnae,ears
R2219 T8406 T8404 prep P12,P10
R2220 T8407 T8408 advmod abnormally,short
R2221 T8408 T8409 amod short,vibrissae
R2222 T8283 T8280 acomp shriveled,were
R2223 T8409 T8403 dobj vibrissae,displayed
R2224 T8410 T8408 cc and,short
R2225 T8411 T8408 conj misshapen,short
R2226 T8284 T8283 prep in,shriveled
R2227 T8412 T8409 cc and,vibrissae
R2228 T8413 T8414 amod short,hairs
R2229 T8414 T8409 conj hairs,vibrissae
R2230 T8285 T8284 pobj appearance,in
R2231 T8415 T8414 punct ", ",hairs
R2232 T8286 T8283 cc and,shriveled
R2233 T8287 T8283 conj pigmented,shriveled
R2234 T8416 T8414 amod shaggy,hairs
R2235 T8288 T8280 prep compared,were
R2236 T8417 T8414 compound pelage,hairs
R2237 T8418 T8419 punct (,2C
R2238 T8289 T8288 prep to,compared
R2239 T8419 T8403 parataxis 2C,displayed
R2240 T8420 T8419 compound Figure,2C
R2241 T8421 T8419 cc and,2C
R2242 T8290 T8289 pobj those,to
R2243 T8422 T8419 conj 2D,2C
R2244 T8423 T8419 punct ),2C
R2245 T8424 T8396 punct .,tended
R2246 T8291 T8290 prep of,those
R2247 T8426 T8427 nsubj Development,became
R2248 T8292 T8291 pobj littermates,of
R2249 T8428 T8426 prep of,Development
R2250 T8429 T8430 amod thick,ridges
R2251 T8293 T8280 punct .,were
R2252 T8430 T8428 pobj ridges,of
R2253 T8431 T8430 prep in,ridges
R2254 T8432 T8433 poss their,skin
R2255 T8295 T8296 amod External,characteristics
R2256 T8433 T8431 pobj skin,in
R2257 T8434 T8430 punct ", ",ridges
R2258 T8435 T8436 advmod particularly,around
R2259 T8436 T8430 prep around,ridges
R2260 T8296 T8297 nsubj characteristics,persisted
R2261 T8437 T8438 det the,ears
R2262 T8438 T8436 pobj ears,around
R2263 T8439 T8438 punct ", ",ears
R2264 T8440 T8438 conj eyelids,ears
R2265 T8441 T8440 punct ", ",eyelids
R2266 T8298 T8296 prep of,characteristics
R2267 T8442 T8440 conj forehead,eyelids
R2268 T8443 T8442 punct ", ",forehead
R2269 T8444 T8442 conj nose,forehead
R2270 T8445 T8444 punct ", ",nose
R2271 T8446 T8444 cc and,nose
R2272 T8447 T8444 conj paws,nose
R2273 T8299 T8300 compound KA,mutants
R2274 T8448 T8427 punct ", ",became
R2275 T8449 T8427 acomp noticeable,became
R2276 T8450 T8451 punct (,2E
R2277 T8300 T8298 pobj mutants,of
R2278 T8301 T8302 dep that,were
R2279 T8451 T8427 parataxis 2E,became
R2280 T8302 T8300 relcl were,mutants
R2281 T8452 T8451 compound Figure,2E
R2282 T8453 T8451 punct ),2E
R2283 T8303 T8302 acomp evident,were
R2284 T8454 T8427 punct .,became
R2285 T8304 T8302 prep at,were
R2286 T8456 T8457 det These,regions
R2287 T8457 T8458 nsubj regions,looked
R2288 T8305 T8304 pobj E18.5,at
R2289 T8306 T8297 prep after,persisted
R2290 T8307 T8306 pobj birth,after
R2291 T8459 T8458 acomp scaly,looked
R2292 T8460 T8458 punct ", ",looked
R2293 T8308 T8297 cc and,persisted
R2294 T8461 T8458 cc and,looked
R2295 T8309 T8297 conj became,persisted
R2296 T8462 T8463 det these,animals
R2297 T8463 T8464 nsubj animals,kept
R2298 T8464 T8458 conj kept,looked
R2299 T8310 T8311 advmod more,prominent
R2300 T8465 T8464 advmod hardly,kept
R2301 T8466 T8467 poss their,eyes
R2302 T8311 T8309 acomp prominent,became
R2303 T8467 T8464 dobj eyes,kept
R2304 T8468 T8464 oprd open,kept
R2305 T8469 T8464 punct .,kept
R2306 T8312 T8313 mark as,grew
R2307 T8471 T8472 prep In,started
R2308 T8313 T8309 advcl grew,became
R2309 T8472 T8490 ccomp started,were
R2310 T8473 T8471 pobj contrast,In
R2311 T8474 T8473 prep to,contrast
R2312 T8314 T8313 nsubj they,grew
R2313 T8475 T8476 det the,littermates
R2314 T8476 T8474 pobj littermates,to
R2315 T8477 T8476 amod normal,littermates
R2316 T8478 T8479 dep that,increased
R2317 T8479 T8476 relcl increased,littermates
R2318 T8480 T8479 advmod consistently,increased
R2319 T8315 T8316 punct (,2A
R2320 T8481 T8482 poss their,weight
R2321 T8482 T8479 dobj weight,increased
R2322 T8483 T8482 compound body,weight
R2323 T8316 T8309 parataxis 2A,became
R2324 T8484 T8479 prep with,increased
R2325 T8485 T8484 pobj age,with
R2326 T8317 T8316 compound Figure,2A
R2327 T8486 T8472 punct ", ",started
R2328 T8487 T8488 amod surviving,mutants
R2329 T8488 T8472 nsubj mutants,started
R2330 T8318 T8319 punct –,2F
R2331 T8489 T8488 compound KA,mutants
R2332 T8491 T8492 aux to,lose
R2333 T8319 T8316 prep 2F,2A
R2334 T8492 T8472 xcomp lose,started
R2335 T8493 T8492 dobj weight,lose
R2336 T8494 T8492 prep from,lose
R2337 T8320 T8316 punct ),2A
R2338 T8495 T8494 pobj P10,from
R2339 T8496 T8495 advmod onwards,P10
R2340 T8497 T8490 punct ;,were
R2341 T8321 T8297 punct .,persisted
R2342 T8498 T8490 prep by,were
R2343 T8499 T8498 pobj P16,by
R2344 T8500 T8501 punct –,P17
R2345 T8323 T8324 nsubjpass Growth,delayed
R2346 T8501 T8499 prep P17,P16
R2347 T8502 T8490 nsubj they,were
R2348 T8503 T8490 advmod all,were
R2349 T8504 T8490 acomp lethargic,were
R2350 T8325 T8323 prep of,Growth
R2351 T8505 T8490 punct ", ",were
R2352 T8506 T8490 cc and,were
R2353 T8507 T8508 nsubj none,survived
R2354 T8508 T8490 conj survived,were
R2355 T8326 T8327 compound pelage,hair
R2356 T8509 T8507 prep of,none
R2357 T8510 T8509 pobj them,of
R2358 T8511 T8508 prep to,survived
R2359 T8327 T8325 pobj hair,of
R2360 T8512 T8511 pobj weaning,to
R2361 T8513 T8514 punct (,2E
R2362 T8514 T8508 parataxis 2E,survived
R2363 T8328 T8324 auxpass was,delayed
R2364 T8515 T8514 compound Figure,2E
R2365 T8516 T8514 cc and,2E
R2366 T8517 T8514 conj 2F,2E
R2367 T8518 T8514 punct ),2E
R2368 T8519 T8490 punct .,were
R2369 T8329 T8324 advmod generally,delayed
R2370 T8521 T8522 prep At,were
R2371 T8330 T8324 prep in,delayed
R2372 T8523 T8524 det the,time
R2373 T8524 T8521 pobj time,At
R2374 T8525 T8524 prep of,time
R2375 T8331 T8332 det the,mutants
R2376 T8526 T8525 pobj autopsy,of
R2377 T8527 T8528 predet all,mutants
R2378 T8528 T8522 nsubj mutants,were
R2379 T8332 T8330 pobj mutants,in
R2380 T8529 T8528 det the,mutants
R2381 T8530 T8522 acomp toothless,were
R2382 T8531 T8522 punct ", ",were
R2383 T8532 T8522 prep without,were
R2384 T8333 T8324 punct .,delayed
R2385 T8533 T8532 pobj incisors,without
R2386 T8534 T8533 cc or,incisors
R2387 T8535 T8533 conj molars,incisors
R2388 T8335 T8336 prep At,were
R2389 T8536 T8522 punct ", ",were
R2390 T8537 T8522 cc and,were
R2391 T8337 T8338 advmod around,P8
R2392 T8538 T8539 poss their,stomachs
R2393 T8539 T8540 nsubj stomachs,were
R2394 T8540 T8522 conj were,were
R2395 T8338 T8335 pobj P8,At
R2396 T8541 T8540 advmod consistently,were
R2397 T8542 T8540 acomp small,were
R2398 T8543 T8540 cc and,were
R2399 T8339 T8336 punct ", ",were
R2400 T8544 T8540 conj had,were
R2401 T8545 T8546 det no,food
R2402 T8340 T8341 det the,mutants
R2403 T8546 T8544 dobj food,had
R2404 T8547 T8546 amod solid,food
R2405 T8341 T8336 nsubj mutants,were
R2406 T8548 T8544 punct ", ",had
R2407 T8549 T8544 prep unlike,had
R2408 T8550 T8551 poss their,littermates
R2409 T8342 T8341 compound KA,mutants
R2410 T8551 T8549 pobj littermates,unlike
R2411 T8552 T8553 npadvmod age,matched
R2412 T8553 T8551 amod matched,littermates
R2413 T8343 T8336 acomp hairless,were
R2414 T8554 T8553 punct -,matched
R2415 T8555 T8522 punct ", ",were
R2416 T8556 T8522 advcl suggesting,were
R2417 T8344 T8336 cc and,were
R2418 T8557 T8558 mark that,be
R2419 T8558 T8556 ccomp be,suggesting
R2420 T8559 T8560 det the,loss
R2421 T8560 T8558 nsubj loss,be
R2422 T8561 T8560 amod observed,loss
R2423 T8345 T8336 conj had,were
R2424 T8562 T8560 compound weight,loss
R2425 T8563 T8558 aux could,be
R2426 T8346 T8347 amod wrinkled,skin
R2427 T8347 T8345 dobj skin,had
R2428 T8564 T8565 det the,result
R2429 T8348 T8349 mark while,had
R2430 T8565 T8558 attr result,be
R2431 T8349 T8336 advcl had,were
R2432 T8566 T8565 prep of,result
R2433 T8567 T8566 pobj failure,of
R2434 T8568 T8569 aux to,ingest
R2435 T8569 T8567 acl ingest,failure
R2436 T8350 T8351 poss their,littermates
R2437 T8570 T8571 amod solid,food
R2438 T8571 T8569 dobj food,ingest
R2439 T8572 T8573 punct (,2F
R2440 T8351 T8349 nsubj littermates,had
R2441 T8573 T8558 parataxis 2F,be
R2442 T8574 T8573 compound Figure,2F
R2443 T8575 T8573 punct ),2F
R2444 T8670 T8669 punct -,mediated
R2445 T8576 T8522 punct .,were
R2446 T8578 T8579 advmod Interestingly,varied
R2447 T8671 T8667 pobj deletion,of
R2448 T8580 T8579 punct ", ",varied
R2449 T8581 T8579 nsubj changes,varied
R2450 T8582 T8581 prep in,changes
R2451 T8672 T8671 compound Apc,deletion
R2452 T8583 T8584 compound body,weights
R2453 T8584 T8582 pobj weights,in
R2454 T8585 T8584 cc and,weights
R2455 T8673 T8655 punct .,suggests
R2456 T8586 T8584 conj timing,weights
R2457 T8587 T8586 prep of,timing
R2458 T8588 T8589 compound hair,growth
R2459 T8675 T8676 nsubj It,is
R2460 T8589 T8587 pobj growth,of
R2461 T8590 T8579 advmod considerably,varied
R2462 T8591 T8579 prep among,varied
R2463 T8592 T8593 amod mutant,pups
R2464 T8677 T8676 acomp possible,is
R2465 T8593 T8591 pobj pups,among
R2466 T8594 T8595 advmod even,were
R2467 T8595 T8579 advcl were,varied
R2468 T8596 T8595 mark if,were
R2469 T8678 T8679 mark that,has
R2470 T8597 T8595 nsubj they,were
R2471 T8598 T8595 prep from,were
R2472 T8599 T8600 det the,litter
R2473 T8600 T8598 pobj litter,from
R2474 T8601 T8600 amod same,litter
R2475 T8602 T8595 punct ", ",were
R2476 T8679 T8676 ccomp has,is
R2477 T8603 T8604 mark whereas,tended
R2478 T8604 T8595 advcl tended,were
R2479 T8605 T8604 nsubj those,tended
R2480 T8680 T8681 det the,background
R2481 T8606 T8605 prep of,those
R2482 T8607 T8608 advmod phenotypically,normal
R2483 T8681 T8679 nsubj background,has
R2484 T8608 T8609 amod normal,littermates
R2485 T8609 T8606 pobj littermates,of
R2486 T8610 T8611 aux to,be
R2487 T8682 T8681 amod genetic,background
R2488 T8611 T8604 xcomp be,tended
R2489 T8612 T8611 acomp similar,be
R2490 T8613 T8579 punct .,varied
R2491 T8683 T8684 det a,role
R2492 T8615 T8616 det This,difference
R2493 T8616 T8617 nsubjpass difference,reflected
R2494 T8684 T8679 dobj role,has
R2495 T8617 T8620 ccomp reflected,survived
R2496 T8618 T8617 auxpass was,reflected
R2497 T8685 T8686 aux to,play
R2498 T8619 T8617 advmod also,reflected
R2499 T8621 T8617 prep in,reflected
R2500 T8686 T8684 advcl play,role
R2501 T8687 T8686 prep in,play
R2502 T8622 T8623 det the,variation
R2503 T8623 T8621 pobj variation,in
R2504 T8688 T8689 det this,variability
R2505 T8624 T8623 prep in,variation
R2506 T8625 T8624 pobj timing,in
R2507 T8626 T8625 prep of,timing
R2508 T8689 T8687 pobj variability,in
R2509 T8627 T8626 pobj death,of
R2510 T8628 T8623 prep in,variation
R2511 T8629 T8628 pobj mutants,in
R2512 T8630 T8620 punct : ,survived
R2513 T8690 T8676 punct .,is
R2514 T8631 T8632 det some,pups
R2515 T8632 T8634 nsubjpass pups,born
R2516 T8692 T8693 amod Gross,examination
R2517 T8633 T8632 amod mutant,pups
R2518 T8634 T8620 ccomp born,survived
R2519 T8635 T8634 auxpass were,born
R2520 T8636 T8634 advcl alive,born
R2521 T8693 T8694 nsubj examination,showed
R2522 T8637 T8634 cc but,born
R2523 T8638 T8634 conj died,born
R2524 T8639 T8638 prep within,died
R2525 T8640 T8641 nummod a,day
R2526 T8641 T8639 pobj day,within
R2527 T8642 T8643 cc or,two
R2528 T8643 T8641 nummod two,day
R2529 T8695 T8693 prep of,examination
R2530 T8644 T8620 punct ", ",survived
R2531 T8645 T8620 nsubj some,survived
R2532 T8646 T8620 advmod close,survived
R2533 T8647 T8646 prep to,close
R2534 T8696 T8697 amod internal,organs
R2535 T8648 T8649 det the,age
R2536 T8649 T8647 pobj age,to
R2537 T8650 T8649 amod weaning,age
R2538 T8697 T8695 pobj organs,of
R2539 T8651 T8620 punct .,survived
R2540 T8698 T8694 advmod also,showed
R2541 T8653 T8654 det This,variability
R2542 T8654 T8655 nsubj variability,suggests
R2543 T8699 T8700 mark that,were
R2544 T8656 T8654 prep of,variability
R2545 T8657 T8658 det the,phenotypes
R2546 T8658 T8656 pobj phenotypes,of
R2547 T8659 T8658 amod mutant,phenotypes
R2548 T8700 T8694 ccomp were,showed
R2549 T8660 T8661 amod possible,variation
R2550 T8661 T8655 dobj variation,suggests
R2551 T8701 T8702 det the,mutants
R2552 T8662 T8661 prep in,variation
R2553 T8663 T8664 det the,timing
R2554 T8664 T8662 pobj timing,in
R2555 T8665 T8664 cc and,timing
R2556 T8702 T8703 poss mutants,thymi
R2557 T8666 T8664 conj efficiency,timing
R2558 T8667 T8664 prep of,timing
R2559 T8668 T8669 npadvmod cre,mediated
R2560 T8703 T8700 nsubj thymi,were
R2561 T8669 T8671 amod mediated,deletion
R2562 T8704 T8702 case ',mutants
R2563 T8705 T8706 advmod consistently,inconspicuous
R2564 T8776 T8774 dobj specimens,preparing
R2565 T8706 T8700 acomp inconspicuous,were
R2566 T8777 T8776 prep of,specimens
R2567 T8778 T8779 nmod P16,mice
R2568 T8779 T8777 pobj mice,of
R2569 T8707 T8700 cc and,were
R2570 T8780 T8781 punct –,P17
R2571 T8781 T8778 prep P17,P16
R2572 T8782 T8779 acl stained,mice
R2573 T8783 T8782 prep with,stained
R2574 T8784 T8785 compound Alizarin,red
R2575 T8785 T8783 pobj red,with
R2576 T8786 T8766 punct .,examined
R2577 T8708 T8700 conj were,were
R2578 T8788 T8789 det No,differences
R2579 T8789 T8790 nsubjpass differences,detected
R2580 T8709 T8710 advmod very,small
R2581 T8791 T8789 prep between,differences
R2582 T8792 T8793 det the,mice
R2583 T8710 T8708 acomp small,were
R2584 T8711 T8710 prep for,small
R2585 T8793 T8791 pobj mice,between
R2586 T8712 T8713 poss their,age
R2587 T8794 T8793 amod normal,mice
R2588 T8795 T8794 cc and,normal
R2589 T8796 T8797 npadvmod KA,mutant
R2590 T8713 T8711 pobj age,for
R2591 T8797 T8794 conj mutant,normal
R2592 T8798 T8789 prep in,differences
R2593 T8799 T8800 det the,bone
R2594 T8714 T8700 punct ", ",were
R2595 T8800 T8798 pobj bone,in
R2596 T8801 T8800 amod mandibular,bone
R2597 T8802 T8790 aux can,detected
R2598 T8715 T8716 mark whereas,were
R2599 T8803 T8790 auxpass be,detected
R2600 T8804 T8790 punct ", ",detected
R2601 T8716 T8700 advcl were,were
R2602 T8805 T8790 cc but,detected
R2603 T8717 T8716 nsubj those,were
R2604 T8806 T8807 det the,mice
R2605 T8807 T8809 nsubj mice,lacked
R2606 T8718 T8717 prep of,those
R2607 T8719 T8720 poss their,littermates
R2608 T8808 T8807 amod mutant,mice
R2609 T8720 T8718 pobj littermates,of
R2610 T8721 T8722 advmod very,prominent
R2611 T8722 T8716 acomp prominent,were
R2612 T8809 T8790 conj lacked,detected
R2613 T8810 T8809 cc or,lacked
R2614 T8723 T8722 prep in,prominent
R2615 T8811 T8809 conj had,lacked
R2616 T8812 T8813 amod underdeveloped,set
R2617 T8813 T8811 dative set,had
R2618 T8724 T8723 pobj size,in
R2619 T8814 T8813 prep of,set
R2620 T8815 T8816 amod maxillary,incisors
R2621 T8816 T8811 dobj incisors,had
R2622 T8725 T8726 punct (,2G
R2623 T8817 T8816 cc and,incisors
R2624 T8818 T8816 conj molars,incisors
R2625 T8819 T8820 punct (,2H
R2626 T8820 T8811 parataxis 2H,had
R2627 T8821 T8820 compound Figure,2H
R2628 T8822 T8820 punct ),2H
R2629 T8726 T8716 parataxis 2G,were
R2630 T8823 T8809 punct .,lacked
R2631 T8825 T8826 nsubj We,detected
R2632 T8727 T8726 compound Figure,2G
R2633 T8827 T8828 det no,abnormalities
R2634 T8828 T8826 dobj abnormalities,detected
R2635 T8728 T8726 punct ),2G
R2636 T8829 T8828 amod other,abnormalities
R2637 T8830 T8828 amod major,abnormalities
R2638 T8729 T8694 punct .,showed
R2639 T8831 T8828 amod skeletal,abnormalities
R2640 T8832 T8826 punct .,detected
R2641 T8731 T8732 det This,difference
R2642 T8732 T8733 nsubj difference,was
R2643 T8734 T8733 acomp evident,was
R2644 T8735 T8736 advmod as,early
R2645 T8736 T8733 advmod early,was
R2646 T8737 T8736 prep as,early
R2647 T8738 T8737 pobj P3,as
R2648 T8739 T8733 punct .,was
R2649 T8741 T8742 advmod Quite,frequently
R2650 T8742 T8743 advmod frequently,contained
R2651 T8744 T8745 amod mutant,thymi
R2652 T8745 T8743 nsubj thymi,contained
R2653 T8746 T8745 prep in,thymi
R2654 T8747 T8748 nmod P12,mice
R2655 T8748 T8746 pobj mice,in
R2656 T8749 T8750 punct –,P17
R2657 T8750 T8747 prep P17,P12
R2658 T8751 T8748 amod mutant,mice
R2659 T8752 T8743 advmod also,contained
R2660 T8753 T8754 amod black,deposits
R2661 T8754 T8743 dobj deposits,contained
R2662 T8755 T8743 prep within,contained
R2663 T8756 T8757 det the,tissue
R2664 T8757 T8755 pobj tissue,within
R2665 T8758 T8759 punct (,data
R2666 T8759 T8743 meta data,contained
R2667 T8760 T8759 amod unpublished,data
R2668 T8761 T8759 punct ),data
R2669 T8762 T8743 punct .,contained
R2670 T8764 T8765 amod Mutant,mice
R2671 T8765 T8766 nsubjpass mice,examined
R2672 T8767 T8766 auxpass were,examined
R2673 T8768 T8766 advmod also,examined
R2674 T8769 T8766 prep for,examined
R2675 T8770 T8771 det any,abnormalities
R2676 T8771 T8769 pobj abnormalities,for
R2677 T8772 T8771 amod skeletal,abnormalities
R2678 T8773 T8766 prep by,examined
R2679 T8774 T8773 pcomp preparing,by
R2680 T8775 T8776 amod skeletal,specimens
R2681 T9607 T9608 npadvmod Genotype,Specific
R2682 T9608 T9613 amod Specific,Expression
R2683 T9609 T9607 punct -,Genotype
R2684 T9610 T9607 cc and,Genotype
R2685 T9611 T9607 conj Tissue,Genotype
R2686 T9612 T9608 punct -,Specific
R2687 T9614 T9613 prep of,Expression
R2688 T9615 T9616 det the,Transcripts
R2689 T9616 T9614 pobj Transcripts,of
R2690 T9617 T9618 amod Truncated,Apc
R2691 T9618 T9616 compound Apc,Transcripts
R2692 T9620 T9621 aux To,assess
R2693 T9621 T9622 advcl assess,screened
R2694 T9623 T9624 det the,effects
R2695 T9624 T9621 dobj effects,assess
R2696 T9625 T9624 amod molecular,effects
R2697 T9626 T9624 prep of,effects
R2698 T9627 T9628 det the,recombination
R2699 T9628 T9626 pobj recombination,of
R2700 T9629 T9630 compound K14,cre
R2701 T9630 T9632 npadvmod cre,mediated
R2702 T9631 T9630 punct -,cre
R2703 T9632 T9628 amod mediated,recombination
R2704 T9633 T9632 punct –,mediated
R2705 T9634 T9622 punct ", ",screened
R2706 T9635 T9622 nsubj we,screened
R2707 T9636 T9622 prep for,screened
R2708 T9637 T9638 det the,presence
R2709 T9638 T9636 pobj presence,for
R2710 T9639 T9638 prep of,presence
R2711 T9640 T9641 amod deleted,Apc
R2712 T9641 T9642 nmod Apc,alleles
R2713 T9642 T9639 pobj alleles,of
R2714 T9643 T9641 punct (,Apc
R2715 T9644 T9641 appos ApcΔ580,Apc
R2716 T9645 T9642 punct ),alleles
R2717 T9646 T9622 punct .,screened
R2718 T9648 T9649 amod Genomic,DNA
R2719 T9649 T9650 nsubjpass DNA,extracted
R2720 T9651 T9650 auxpass was,extracted
R2721 T9652 T9650 prep from,extracted
R2722 T9653 T9652 pobj liver,from
R2723 T9654 T9653 punct ", ",liver
R2724 T9655 T9653 conj thymus,liver
R2725 T9656 T9655 punct ", ",thymus
R2726 T9657 T9655 cc and,thymus
R2727 T9658 T9655 conj skin,thymus
R2728 T9659 T9653 prep from,liver
R2729 T9660 T9661 det all,genotypes
R2730 T9661 T9659 pobj genotypes,from
R2731 T9662 T9661 nummod 4,genotypes
R2732 T9663 T9661 amod possible,genotypes
R2733 T9664 T9661 punct : ,genotypes
R2734 T9665 T9666 compound K14,cre
R2735 T9666 T9661 appos cre,genotypes
R2736 T9667 T9666 punct -,cre
R2737 T9668 T9666 punct ;,cre
R2738 T9669 T9670 compound ApcCKO,CKO
R2739 T9670 T9666 appos CKO,cre
R2740 T9671 T9670 punct /,CKO
R2741 T9672 T9666 punct ", ",cre
R2742 T9673 T9674 compound K14,cre
R2743 T9674 T9666 conj cre,cre
R2744 T9675 T9674 punct -,cre
R2745 T9676 T9674 punct ;,cre
R2746 T9677 T9674 appos ApcCKO,cre
R2747 T9678 T9677 punct /,ApcCKO
R2748 T9679 T9677 punct +,ApcCKO
R2749 T9680 T9674 punct ", ",cre
R2750 T9681 T9682 compound ApcCKO,CKO
R2751 T9682 T9674 conj CKO,cre
R2752 T9683 T9682 punct /,CKO
R2753 T9684 T9682 punct ", ",CKO
R2754 T9685 T9682 cc and,CKO
R2755 T9686 T9682 conj ApcCKO,CKO
R2756 T9687 T9686 punct /,ApcCKO
R2757 T9688 T9686 punct +,ApcCKO
R2758 T9689 T9650 punct .,extracted
R2759 T9691 T9692 nsubj Genotyping,showed
R2760 T9693 T9691 prep on,Genotyping
R2761 T9694 T9695 amod genomic,DNA
R2762 T9695 T9693 pobj DNA,on
R2763 T9696 T9695 prep from,DNA
R2764 T9697 T9698 det these,tissues
R2765 T9698 T9696 pobj tissues,from
R2766 T9699 T9700 mark that,detected
R2767 T9700 T9692 ccomp detected,showed
R2768 T9701 T9702 det the,allele
R2769 T9702 T9700 nsubjpass allele,detected
R2770 T9703 T9702 compound ApcΔ580,allele
R2771 T9704 T9705 punct (,product
R2772 T9705 T9702 parataxis product,allele
R2773 T9706 T9707 nummod 500,bp
R2774 T9707 T9705 compound bp,product
R2775 T9708 T9707 punct -,bp
R2776 T9709 T9705 punct ),product
R2777 T9710 T9700 auxpass was,detected
R2778 T9711 T9712 advmod only,from
R2779 T9712 T9700 prep from,detected
R2780 T9713 T9714 det the,skin
R2781 T9714 T9712 pobj skin,from
R2782 T9715 T9714 cc and,skin
R2783 T9716 T9714 conj thymus,skin
R2784 T9717 T9714 prep of,skin
R2785 T9718 T9719 det the,mice
R2786 T9719 T9717 pobj mice,of
R2787 T9720 T9721 compound K14,cre
R2788 T9721 T9723 npadvmod cre,positive
R2789 T9722 T9721 punct -,cre
R2790 T9723 T9719 amod positive,mice
R2791 T9724 T9723 punct –,positive
R2792 T9725 T9692 punct .,showed
R2793 T9727 T9728 det The,presence
R2794 T9728 T9729 nsubj presence,was
R2795 T9730 T9728 prep of,presence
R2796 T9731 T9732 amod mutant,allele
R2797 T9732 T9730 pobj allele,of
R2798 T9733 T9732 compound Apc,allele
R2799 T9734 T9732 prep in,allele
R2800 T9735 T9736 det the,thymus
R2801 T9736 T9734 pobj thymus,in
R2802 T9737 T9736 prep of,thymus
R2803 T9738 T9739 nmod K14,cre
R2804 T9739 T9741 nmod cre,mice
R2805 T9740 T9739 punct -,cre
R2806 T9741 T9737 pobj mice,of
R2807 T9742 T9739 punct ;,cre
R2808 T9743 T9739 appos ApcCKO,cre
R2809 T9744 T9743 punct /,ApcCKO
R2810 T9745 T9743 punct +,ApcCKO
R2811 T9746 T9729 advmod consistently,was
R2812 T9747 T9748 advmod much,less
R2813 T9748 T9729 acomp less,was
R2814 T9749 T9748 prep than,less
R2815 T9750 T9751 det the,DNA
R2816 T9751 T9749 pobj DNA,than
R2817 T9752 T9751 prep from,DNA
R2818 T9753 T9754 det the,skin
R2819 T9754 T9752 pobj skin,from
R2820 T9755 T9754 prep of,skin
R2821 T9756 T9757 det the,animal
R2822 T9757 T9755 pobj animal,of
R2823 T9758 T9757 amod same,animal
R2824 T9759 T9751 cc or,DNA
R2825 T9760 T9761 amod other,tissues
R2826 T9761 T9751 conj tissues,DNA
R2827 T9762 T9761 prep from,tissues
R2828 T9763 T9764 det the,mutants
R2829 T9764 T9762 pobj mutants,from
R2830 T9765 T9764 compound KA,mutants
R2831 T9766 T9729 punct .,was
R2832 T9768 T9769 prep In,detected
R2833 T9770 T9768 pobj addition,In
R2834 T9771 T9769 punct ", ",detected
R2835 T9772 T9773 det this,product
R2836 T9773 T9769 nsubjpass product,detected
R2837 T9774 T9769 auxpass was,detected
R2838 T9775 T9769 neg not,detected
R2839 T9776 T9777 advmod at,all
R2840 T9777 T9769 advmod all,detected
R2841 T9778 T9769 prep in,detected
R2842 T9779 T9780 preconj either,liver
R2843 T9780 T9778 pobj liver,in
R2844 T9781 T9780 det the,liver
R2845 T9782 T9780 prep of,liver
R2846 T9783 T9784 compound K14,cre
R2847 T9784 T9786 npadvmod cre,positive
R2848 T9785 T9784 punct -,cre
R2849 T9786 T9782 amod positive,of
R2850 T9787 T9786 punct –,positive
R2851 T9788 T9778 cc or,in
R2852 T9789 T9778 conj in,in
R2853 T9790 T9789 pobj any,in
R2854 T9791 T9790 prep of,any
R2855 T9792 T9793 det the,negative
R2856 T9793 T9791 pobj negative,of
R2857 T9794 T9795 compound K14,cre
R2858 T9795 T9793 npadvmod cre,negative
R2859 T9796 T9795 punct -,cre
R2860 T9797 T9793 punct –,negative
R2861 T9798 T9799 compound mouse,samples
R2862 T9799 T9789 pobj samples,in
R2863 T9800 T9799 compound tissues,samples
R2864 T9801 T9769 punct ", ",detected
R2865 T9802 T9769 advcl establishing,detected
R2866 T9803 T9804 mark that,taken
R2867 T9804 T9802 ccomp taken,establishing
R2868 T9805 T9806 npadvmod Cre,mediated
R2869 T9806 T9808 amod mediated,recombination
R2870 T9807 T9806 punct -,mediated
R2871 T9808 T9804 nsubj recombination,taken
R2872 T9809 T9804 aux has,taken
R2873 T9810 T9804 dobj place,taken
R2874 T9811 T9804 prep in,taken
R2875 T9812 T9813 det the,manner
R2876 T9813 T9811 pobj manner,in
R2877 T9814 T9815 npadvmod tissue,specific
R2878 T9815 T9813 amod specific,manner
R2879 T9816 T9815 punct -,specific
R2880 T9817 T9804 prep in,taken
R2881 T9818 T9819 det the,mice
R2882 T9819 T9817 pobj mice,in
R2883 T9820 T9821 dep that,inherited
R2884 T9821 T9819 relcl inherited,mice
R2885 T9822 T9823 compound K14,cre
R2886 T9823 T9821 dobj cre,inherited
R2887 T9824 T9823 punct -,cre
R2888 T9825 T9826 punct (,3A
R2889 T9826 T9769 parataxis 3A,detected
R2890 T9827 T9826 compound Figure,3A
R2891 T9828 T9826 punct ),3A
R2892 T9829 T9769 punct .,detected
R2893 T9831 T9832 compound Apc,transcripts
R2894 T9832 T9833 nsubjpass transcripts,analyzed
R2895 T9834 T9833 auxpass were,analyzed
R2896 T9835 T9833 advmod also,analyzed
R2897 T9836 T9833 prep by,analyzed
R2898 T9837 T9838 compound RT,PCR
R2899 T9838 T9836 pobj PCR,by
R2900 T9839 T9838 punct -,PCR
R2901 T9840 T9838 prep with,PCR
R2902 T9841 T9840 pobj primers,with
R2903 T9842 T9841 acl spanning,primers
R2904 T9843 T9842 dobj exon,spanning
R2905 T9844 T9843 nummod 14,exon
R2906 T9845 T9846 punct (,1A
R2907 T9846 T9833 parataxis 1A,analyzed
R2908 T9847 T9846 compound Figure,1A
R2909 T9848 T9846 punct ),1A
R2910 T9849 T9833 advcl using,analyzed
R2911 T9850 T9851 amod total,RNA
R2912 T9851 T9849 dobj RNA,using
R2913 T9852 T9851 acl isolated,RNA
R2914 T9853 T9852 prep from,isolated
R2915 T9854 T9855 det the,samples
R2916 T9855 T9853 pobj samples,from
R2917 T9856 T9855 amod corresponding,samples
R2918 T9857 T9855 compound tissue,samples
R2919 T9858 T9833 punct .,analyzed
R2920 T9860 T9861 nsubj We,detected
R2921 T9862 T9863 det the,product
R2922 T9863 T9861 dobj product,detected
R2923 T9864 T9863 amod expected,product
R2924 T9865 T9866 compound RT,PCR
R2925 T9866 T9863 compound PCR,product
R2926 T9867 T9866 punct -,PCR
R2927 T9868 T9869 punct (,bp
R2928 T9869 T9863 parataxis bp,product
R2929 T9870 T9869 nummod 313,bp
R2930 T9871 T9869 punct ),bp
R2931 T9872 T9863 prep from,product
R2932 T9873 T9874 det the,allele
R2933 T9874 T9872 pobj allele,from
R2934 T9875 T9876 amod truncated,Apc
R2935 T9876 T9874 nmod Apc,allele
R2936 T9877 T9876 punct (,Apc
R2937 T9878 T9876 appos ApcΔ580,Apc
R2938 T9879 T9874 punct ),allele
R2939 T9880 T9881 advmod only,in
R2940 T9881 T9861 prep in,detected
R2941 T9882 T9883 det the,tissues
R2942 T9883 T9881 pobj tissues,in
R2943 T9884 T9885 advmod where,known
R2944 T9885 T9883 advcl known,tissues
R2945 T9886 T9887 compound Cre,recombinase
R2946 T9887 T9885 nsubjpass recombinase,known
R2947 T9888 T9885 auxpass is,known
R2948 T9889 T9890 aux to,expressed
R2949 T9890 T9885 xcomp expressed,known
R2950 T9891 T9890 auxpass be,expressed
R2951 T9892 T9890 prep in,expressed
R2952 T9893 T9894 det the,mice
R2953 T9894 T9892 pobj mice,in
R2954 T9895 T9896 compound K14,cre
R2955 T9896 T9898 npadvmod cre,positive
R2956 T9897 T9896 punct -,cre
R2957 T9898 T9894 amod positive,mice
R2958 T9899 T9898 punct –,positive
R2959 T9900 T9861 punct .,detected
R2960 T9902 T9903 advmod However,detected
R2961 T9904 T9903 punct ", ",detected
R2962 T9905 T9906 det this,product
R2963 T9906 T9903 nsubjpass product,detected
R2964 T9907 T9903 auxpass was,detected
R2965 T9908 T9903 neg not,detected
R2966 T9909 T9903 prep in,detected
R2967 T9910 T9911 preconj either,samples
R2968 T9911 T9909 pobj samples,in
R2969 T9912 T9911 det the,samples
R2970 T9913 T9914 compound K14,cre
R2971 T9914 T9916 npadvmod cre,negative
R2972 T9915 T9914 punct -,cre
R2973 T9916 T9911 amod negative,samples
R2974 T9917 T9916 punct –,negative
R2975 T9918 T9919 compound mouse,tissues
R2976 T9919 T9911 compound tissues,samples
R2977 T9920 T9911 cc or,samples
R2978 T9921 T9922 det the,liver
R2979 T9922 T9911 conj liver,samples
R2980 T9923 T9922 prep of,liver
R2981 T9924 T9925 compound K14,cre
R2982 T9925 T9927 npadvmod cre,positive
R2983 T9926 T9925 punct -,cre
R2984 T9927 T9929 amod positive,mice
R2985 T9928 T9927 punct –,positive
R2986 T9929 T9923 pobj mice,of
R2987 T9930 T9903 punct ", ",detected
R2988 T9931 T9903 cc and,detected
R2989 T9932 T9933 advmod only,product
R2990 T9933 T9935 nsubjpass product,detected
R2991 T9934 T9933 det the,product
R2992 T9935 T9903 conj detected,detected
R2993 T9936 T9933 prep from,product
R2994 T9937 T9938 det the,allele
R2995 T9938 T9936 pobj allele,from
R2996 T9939 T9940 amod wild,type
R2997 T9940 T9938 compound type,allele
R2998 T9941 T9940 punct -,type
R2999 T9942 T9943 punct (,bp
R3000 T9943 T9933 parataxis bp,product
R3001 T9944 T9943 nummod 528,bp
R3002 T9945 T9943 punct ),bp
R3003 T9946 T9935 auxpass was,detected
R3004 T9947 T9935 prep from,detected
R3005 T9948 T9949 det these,samples
R3006 T9949 T9947 pobj samples,from
R3007 T9950 T9949 compound RNA,samples
R3008 T9951 T9935 punct ", ",detected
R3009 T9952 T9953 advmod further,confirming
R3010 T9953 T9935 advcl confirming,detected
R3011 T9954 T9955 mark that,taken
R3012 T9955 T9953 ccomp taken,confirming
R3013 T9956 T9957 npadvmod Cre,mediated
R3014 T9957 T9959 amod mediated,recombination
R3015 T9958 T9957 punct -,mediated
R3016 T9959 T9955 nsubj recombination,taken
R3017 T9960 T9955 aux has,taken
R3018 T9961 T9955 dobj place,taken
R3019 T9962 T9955 prep in,taken
R3020 T9963 T9964 det the,manner
R3021 T9964 T9962 pobj manner,in
R3022 T9965 T9966 npadvmod tissue,specific
R3023 T9966 T9964 amod specific,manner
R3024 T9967 T9965 punct -,tissue
R3025 T9968 T9965 cc and,tissue
R3026 T9969 T9965 conj genotype,tissue
R3027 T9970 T9966 punct -,specific
R3028 T9971 T9972 punct (,3B
R3029 T9972 T9935 parataxis 3B,detected
R3030 T9973 T9972 compound Figure,3B
R3031 T9974 T9972 punct ),3B
R3032 T9975 T9903 punct .,detected
R3047 T12568 T12569 amod K14,cre
R3048 T12569 T12571 amod cre,Driven
R3049 T12570 T12569 punct -,cre
R3050 T12571 T12573 amod Driven,Follicles
R3051 T12572 T12571 punct –,Driven
R3052 T12574 T12575 compound Apc,Loss
R3053 T12575 T12576 npadvmod Loss,Induced
R3054 T12576 T12573 amod Induced,Follicles
R3055 T12577 T12573 compound Aberrant,Follicles
R3056 T12578 T12573 compound Hair,Follicles
R3057 T12579 T12573 prep throughout,Follicles
R3058 T12580 T12581 det the,Epidermis
R3059 T12581 T12579 pobj Epidermis,throughout
R3060 T12583 T12584 aux To,understand
R3061 T12584 T12585 advcl understand,conducted
R3062 T12586 T12587 det the,basis
R3063 T12587 T12584 dobj basis,understand
R3064 T12588 T12587 prep for,basis
R3065 T12589 T12590 amod delayed,development
R3066 T12590 T12588 pobj development,for
R3067 T12591 T12589 cc and,delayed
R3068 T12592 T12589 conj abnormal,delayed
R3069 T12593 T12590 compound hair,development
R3070 T12594 T12590 prep in,development
R3071 T12595 T12596 det the,mutants
R3072 T12596 T12594 pobj mutants,in
R3073 T12597 T12596 compound KA,mutants
R3074 T12598 T12585 punct ", ",conducted
R3075 T12599 T12585 nsubj we,conducted
R3076 T12600 T12601 det a,examination
R3077 T12601 T12585 dobj examination,conducted
R3078 T12602 T12601 amod histological,examination
R3079 T12603 T12602 cc and,histological
R3080 T12604 T12602 conj immunohistochemical,histological
R3081 T12605 T12606 punct (,Figure
R3082 T12606 T12585 parataxis Figure,conducted
R3083 T12607 T12606 nummod 4,Figure
R3084 T12608 T12606 punct ),Figure
R3085 T12609 T12585 punct .,conducted
R3086 T12611 T12612 det The,follicle
R3087 T12612 T12614 nsubj follicle,is
R3088 T12613 T12612 compound hair,follicle
R3089 T12615 T12616 det an,appendage
R3090 T12616 T12614 attr appendage,is
R3091 T12617 T12616 amod epidermal,appendage
R3092 T12618 T12619 dep that,consists
R3093 T12619 T12616 relcl consists,appendage
R3094 T12620 T12619 prep of,consists
R3095 T12621 T12622 det an,portion
R3096 T12622 T12620 pobj portion,of
R3097 T12623 T12622 amod upper,portion
R3098 T12624 T12622 amod permanent,portion
R3099 T12625 T12622 punct ", ",portion
R3100 T12626 T12622 cc and,portion
R3101 T12627 T12628 det a,portion
R3102 T12628 T12622 conj portion,portion
R3103 T12629 T12628 amod lower,portion
R3104 T12630 T12628 compound cycling,portion
R3105 T12631 T12632 dep that,produces
R3106 T12632 T12628 relcl produces,portion
R3107 T12633 T12634 det the,hair
R3108 T12634 T12632 dobj hair,produces
R3109 T12635 T12636 punct [,23
R3110 T12636 T12614 parataxis 23,is
R3111 T12637 T12636 nummod 22,23
R3112 T12638 T12636 punct ",",23
R3113 T12639 T12636 punct ],23
R3114 T12640 T12614 punct .,is
R3115 T12642 T12643 det The,sheath
R3116 T12643 T12646 nsubj sheath,is
R3117 T12644 T12643 amod outer,sheath
R3118 T12645 T12643 compound root,sheath
R3119 T12647 T12643 punct (,sheath
R3120 T12648 T12643 appos ORS,sheath
R3121 T12649 T12646 punct ),is
R3122 T12650 T12646 acomp contiguous,is
R3123 T12651 T12650 prep with,contiguous
R3124 T12652 T12650 cc and,contiguous
R3125 T12653 T12654 advmod biochemically,similar
R3126 T12654 T12650 conj similar,contiguous
R3127 T12655 T12654 prep to,similar
R3128 T12656 T12657 det the,layer
R3129 T12657 T12654 npadvmod layer,similar
R3130 T12658 T12657 amod basal,layer
R3131 T12659 T12657 prep of,layer
R3132 T12660 T12661 det the,epidermis
R3133 T12661 T12659 pobj epidermis,of
R3134 T12662 T12646 punct .,is
R3135 T12664 T12665 det The,layers
R3136 T12665 T12667 nsubj layers,include
R3137 T12666 T12665 amod inner,layers
R3138 T12668 T12665 prep of,layers
R3139 T12669 T12670 det the,follicle
R3140 T12670 T12668 pobj follicle,of
R3141 T12671 T12670 compound hair,follicle
R3142 T12672 T12673 nummod three,layers
R3143 T12673 T12667 dobj layers,include
R3144 T12674 T12673 amod concentric,layers
R3145 T12675 T12673 prep of,layers
R3146 T12676 T12677 amod inner,sheath
R3147 T12677 T12675 pobj sheath,of
R3148 T12678 T12677 compound root,sheath
R3149 T12679 T12673 cc and,layers
R3150 T12680 T12681 nummod three,layers
R3151 T12681 T12673 conj layers,layers
R3152 T12682 T12681 amod concentric,layers
R3153 T12683 T12681 prep of,layers
R3154 T12684 T12685 npadvmod hair,producing
R3155 T12685 T12687 amod producing,cells
R3156 T12686 T12685 punct -,producing
R3157 T12687 T12683 pobj cells,of
R3158 T12688 T12667 punct .,include
R3159 T12690 T12691 prep At,is
R3160 T12692 T12693 det the,base
R3161 T12693 T12690 pobj base,At
R3162 T12694 T12693 prep of,base
R3163 T12695 T12696 det the,follicle
R3164 T12696 T12694 pobj follicle,of
R3165 T12697 T12696 compound hair,follicle
R3166 T12698 T12699 det the,bulb
R3167 T12699 T12691 nsubj bulb,is
R3168 T12700 T12699 amod germinative,bulb
R3169 T12701 T12702 compound hair,follicle
R3170 T12702 T12699 compound follicle,bulb
R3171 T12703 T12699 punct ", ",bulb
R3172 T12704 T12705 dep which,contains
R3173 T12705 T12699 relcl contains,bulb
R3174 T12706 T12707 advmod rapidly,proliferating
R3175 T12707 T12708 amod proliferating,cells
R3176 T12708 T12705 dobj cells,contains
R3177 T12709 T12708 punct “,cells
R3178 T12710 T12708 nmod matrix,cells
R3179 T12711 T12708 punct ”,cells
R3180 T12712 T12713 dep that,differentiate
R3181 T12713 T12708 relcl differentiate,cells
R3182 T12714 T12715 aux to,populate
R3183 T12715 T12713 advcl populate,differentiate
R3184 T12716 T12715 dobj all,populate
R3185 T12717 T12716 prep of,all
R3186 T12718 T12719 det the,layers
R3187 T12719 T12717 pobj layers,of
R3188 T12720 T12719 prep of,layers
R3189 T12721 T12722 det the,sheath
R3190 T12722 T12720 pobj sheath,of
R3191 T12723 T12722 amod inner,sheath
R3192 T12724 T12722 compound root,sheath
R3193 T12725 T12716 cc and,all
R3194 T12726 T12727 det the,shaft
R3195 T12727 T12716 conj shaft,all
R3196 T12728 T12727 compound hair,shaft
R3197 T12729 T12727 appos itself,shaft
R3198 T12730 T12731 punct [,22
R3199 T12731 T12691 parataxis 22,is
R3200 T12732 T12731 punct ],22
R3201 T12733 T12691 punct .,is
R3202 T12735 T12736 prep During,grew
R3203 T12737 T12738 det the,phase
R3204 T12738 T12735 pobj phase,During
R3205 T12739 T12738 compound anagen,phase
R3206 T12740 T12738 prep of,phase
R3207 T12741 T12742 det the,cycle
R3208 T12742 T12740 pobj cycle,of
R3209 T12743 T12742 compound hair,cycle
R3210 T12744 T12745 punct (,until
R3211 T12745 T12738 parataxis until,phase
R3212 T12746 T12745 pobj P15,until
R3213 T12747 T12745 punct ),until
R3214 T12748 T12736 punct ", ",grew
R3215 T12749 T12750 compound hair,follicles
R3216 T12750 T12736 nsubj follicles,grew
R3217 T12751 T12750 prep of,follicles
R3218 T12752 T12753 advmod phenotypically,mice
R3219 T12753 T12751 pobj mice,of
R3220 T12754 T12753 amod normal,mice
R3221 T12755 T12736 advmod deeply,grew
R3222 T12756 T12736 prep into,grew
R3223 T12757 T12758 det the,fat
R3224 T12758 T12756 pobj fat,into
R3225 T12759 T12758 amod subcutaneous,fat
R3226 T12760 T12736 cc and,grew
R3227 T12761 T12762 auxpass were,spaced
R3228 T12762 T12736 conj spaced,grew
R3229 T12763 T12762 advmod uniformly,spaced
R3230 T12764 T12762 cc and,spaced
R3231 T12765 T12762 conj aligned,spaced
R3232 T12766 T12765 prep in,aligned
R3233 T12767 T12768 amod parallel,arrays
R3234 T12768 T12766 pobj arrays,in
R3235 T12769 T12765 prep at,aligned
R3236 T12770 T12771 det a,angle
R3237 T12771 T12769 pobj angle,at
R3238 T12772 T12771 amod specific,angle
R3239 T12773 T12771 amod relative,angle
R3240 T12774 T12773 prep to,relative
R3241 T12775 T12776 det the,surface
R3242 T12776 T12774 pobj surface,to
R3243 T12777 T12776 compound skin,surface
R3244 T12778 T12779 punct (,4A
R3245 T12779 T12765 parataxis 4A,aligned
R3246 T12780 T12779 compound Figure,4A
R3247 T12781 T12779 punct ),4A
R3248 T12782 T12736 punct .,grew
R3249 T12784 T12785 prep In,spaced
R3250 T12786 T12784 pobj contrast,In
R3251 T12787 T12785 punct ", ",spaced
R3252 T12788 T12789 nmod KA,follicles
R3253 T12789 T12785 nsubjpass follicles,spaced
R3254 T12790 T12789 amod mutant,follicles
R3255 T12791 T12785 auxpass were,spaced
R3256 T12792 T12785 advmod irregularly,spaced
R3257 T12793 T12785 cc and,spaced
R3258 T12794 T12795 advmod often,seen
R3259 T12795 T12785 conj seen,spaced
R3260 T12796 T12795 prep as,seen
R3261 T12797 T12798 amod disoriented,invaginations
R3262 T12798 T12796 pobj invaginations,as
R3263 T12799 T12797 cc and,disoriented
R3264 T12800 T12797 conj clamped,disoriented
R3265 T12801 T12798 prep at,invaginations
R3266 T12802 T12801 pobj P3,at
R3267 T12803 T12804 dep that,became
R3268 T12804 T12798 relcl became,invaginations
R3269 T12805 T12806 advmod even,more
R3270 T12806 T12807 advmod more,remarkable
R3271 T12807 T12804 acomp remarkable,became
R3272 T12808 T12804 prep at,became
R3273 T12809 T12808 pobj P12,at
R3274 T12810 T12811 advmod when,covered
R3275 T12811 T12809 advcl covered,P12
R3276 T12812 T12813 det the,mice
R3277 T12813 T12811 nsubjpass mice,covered
R3278 T12814 T12813 amod mutant,mice
R3279 T12815 T12811 auxpass were,covered
R3280 T12816 T12811 agent by,covered
R3281 T12817 T12818 compound fur,coat
R3282 T12818 T12816 pobj coat,by
R3283 T12819 T12820 punct (,4F
R3284 T12820 T12795 parataxis 4F,seen
R3285 T12821 T12820 compound Figure,4F
R3286 T12822 T12820 punct ),4F
R3287 T12823 T12785 punct .,spaced
R3288 T12825 T12826 nsubj Bulbs,were
R3289 T12827 T12826 advmod often,were
R3290 T12828 T12826 acomp bent,were
R3291 T12829 T12826 prep in,were
R3292 T12830 T12829 pobj addition,in
R3293 T12831 T12830 prep to,addition
R3294 T12832 T12831 pcomp being,to
R3295 T12833 T12834 advmod irregularly,angled
R3296 T12834 T12832 acomp angled,being
R3297 T12835 T12834 prep to,angled
R3298 T12836 T12837 compound one,another
R3299 T12837 T12835 pobj another,to
R3300 T12838 T12826 cc and,were
R3301 T12839 T12840 poss their,sizes
R3302 T12840 T12841 nsubj sizes,were
R3303 T12841 T12826 conj were,were
R3304 T12842 T12840 cc and,sizes
R3305 T12843 T12840 conj locations,sizes
R3306 T12844 T12841 advmod often,were
R3307 T12845 T12841 acomp variable,were
R3308 T12846 T12841 punct .,were
R3309 T12848 T12849 nsubjpass Clusters,observed
R3310 T12850 T12848 prep of,Clusters
R3311 T12851 T12852 amod multiple,invaginations
R3312 T12852 T12850 pobj invaginations,of
R3313 T12853 T12852 cc or,invaginations
R3314 T12854 T12855 amod dysplastic,structures
R3315 T12855 T12852 conj structures,invaginations
R3316 T12856 T12855 amod follicular,structures
R3317 T12857 T12849 auxpass were,observed
R3318 T12858 T12849 advmod frequently,observed
R3319 T12859 T12849 prep throughout,observed
R3320 T12860 T12861 det the,epidermis
R3321 T12861 T12859 pobj epidermis,throughout
R3322 T12862 T12849 punct ", ",observed
R3323 T12863 T12864 mark whereas,showed
R3324 T12864 T12849 advcl showed,observed
R3325 T12865 T12866 amod other,regions
R3326 T12866 T12864 nsubj regions,showed
R3327 T12867 T12864 dobj gaps,showed
R3328 T12868 T12867 prep with,gaps
R3329 T12869 T12870 det no,follicles
R3330 T12870 T12868 pobj follicles,with
R3331 T12871 T12849 punct .,observed
R3332 T12873 T12874 amod Serial,sectioning
R3333 T12874 T12875 nsubj sectioning,indicated
R3334 T12876 T12877 mark that,were
R3335 T12877 T12875 ccomp were,indicated
R3336 T12878 T12877 nsubj some,were
R3337 T12879 T12878 prep of,some
R3338 T12880 T12881 det the,follicles
R3339 T12881 T12879 pobj follicles,of
R3340 T12882 T12881 compound hair,follicles
R3341 T12883 T12881 prep in,follicles
R3342 T12884 T12885 det the,skin
R3343 T12885 T12883 pobj skin,in
R3344 T12886 T12885 nmod P12,skin
R3345 T12887 T12885 amod mutant,skin
R3346 T12888 T12877 neg not,were
R3347 T12889 T12890 advmod properly,formed
R3348 T12890 T12877 acomp formed,were
R3349 T12891 T12877 cc or,were
R3350 T12892 T12877 conj shorter,were
R3351 T12893 T12892 prep than,shorter
R3352 T12894 T12893 amod normal,than
R3353 T12895 T12875 punct .,indicated
R3354 T12897 T12898 advcl Taken,account
R3355 T12899 T12897 advmod together,Taken
R3356 T12900 T12898 punct ", ",account
R3357 T12901 T12902 det these,features
R3358 T12902 T12898 nsubj features,account
R3359 T12903 T12898 aux could,account
R3360 T12904 T12898 prep for,account
R3361 T12905 T12906 det the,delayed
R3362 T12906 T12904 pobj delayed,for
R3363 T12907 T12906 advmod apparently,delayed
R3364 T12908 T12906 punct ", ",delayed
R3365 T12909 T12906 acl followed,delayed
R3366 T12910 T12909 agent by,followed
R3367 T12911 T12910 pobj outgrowth,by
R3368 T12912 T12906 punct ", ",delayed
R3369 T12913 T12906 prep of,delayed
R3370 T12914 T12915 det the,coat
R3371 T12915 T12913 pobj coat,of
R3372 T12916 T12915 amod short,coat
R3373 T12917 T12916 cc and,short
R3374 T12918 T12919 amod shaggy,looking
R3375 T12919 T12916 conj looking,short
R3376 T12920 T12919 punct -,looking
R3377 T12921 T12915 compound fur,coat
R3378 T12922 T12915 prep of,coat
R3379 T12923 T12924 det these,mice
R3380 T12924 T12922 pobj mice,of
R3381 T12925 T12924 amod mutant,mice
R3382 T12926 T12898 punct .,account
R3383 T12928 T12929 nsubj Apc,is
R3384 T12930 T12931 det a,regulator
R3385 T12931 T12929 attr regulator,is
R3386 T12932 T12931 prep of,regulator
R3387 T12933 T12934 compound β,catenin
R3388 T12934 T12932 pobj catenin,of
R3389 T12935 T12934 punct -,catenin
R3390 T12936 T12937 dep that,is
R3391 T12937 T12931 relcl is,regulator
R3392 T12938 T12937 acomp important,is
R3393 T12939 T12938 prep for,important
R3394 T12940 T12941 compound Wnt,signaling
R3395 T12941 T12939 pobj signaling,for
R3396 T12942 T12929 punct .,is
R3397 T12944 T12945 nsubj We,examined
R3398 T12946 T12947 det the,patterns
R3399 T12947 T12945 dobj patterns,examined
R3400 T12948 T12947 prep of,patterns
R3401 T12949 T12948 pobj expression,of
R3402 T12950 T12949 prep of,expression
R3403 T12951 T12952 compound β,catenin
R3404 T12952 T12950 pobj catenin,of
R3405 T12953 T12952 punct -,catenin
R3406 T12954 T12945 prep in,examined
R3407 T12955 T12956 det the,tissues
R3408 T12956 T12954 pobj tissues,in
R3409 T12957 T12956 amod affected,tissues
R3410 T12958 T12945 punct .,examined
R3411 T12960 T12961 prep In,found
R3412 T12962 T12963 det the,skin
R3413 T12963 T12960 pobj skin,In
R3414 T12964 T12963 amod normal,skin
R3415 T12965 T12961 punct ", ",found
R3416 T12966 T12967 compound β,catenin
R3417 T12967 T12961 nsubjpass catenin,found
R3418 T12968 T12967 punct -,catenin
R3419 T12969 T12967 punct ", ",catenin
R3420 T13040 T13038 prep of,patterns
R3421 T12970 T12971 det a,member
R3422 T13041 T13040 pobj expression,of
R3423 T12971 T12967 appos member,catenin
R3424 T13042 T13041 prep of,expression
R3425 T13043 T13042 pobj K14,of
R3426 T12972 T12971 prep of,member
R3427 T13044 T13043 punct ", ",K14
R3428 T13045 T13043 conj K1,K14
R3429 T13046 T13045 punct ", ",K1
R3430 T12973 T12974 det the,complex
R3431 T13047 T13045 conj involucrin,K1
R3432 T13048 T13047 punct ", ",involucrin
R3433 T12974 T12972 pobj complex,of
R3434 T13049 T13047 cc and,involucrin
R3435 T13050 T13047 conj loricrin,involucrin
R3436 T13051 T13041 punct ", ",expression
R3437 T12975 T12976 compound adherens,junction
R3438 T13052 T13041 prep in,expression
R3439 T13053 T13052 pobj skin,in
R3440 T13054 T13053 prep from,skin
R3441 T12976 T12974 compound junction,complex
R3442 T13055 T13056 amod mutant,littermate
R3443 T13056 T13059 nmod littermate,mice
R3444 T13057 T13055 cc and,mutant
R3445 T13058 T13055 conj normal,mutant
R3446 T13059 T13054 pobj mice,from
R3447 T13060 T13041 prep at,expression
R3448 T13061 T13060 pobj P3,at
R3449 T12977 T12961 punct ", ",found
R3450 T13062 T13063 punct –,P17
R3451 T13063 T13061 prep P17,P3
R3452 T13064 T13039 punct ", ",showed
R3453 T12978 T12961 auxpass was,found
R3454 T13065 T13066 det no,differences
R3455 T13066 T13039 dobj differences,showed
R3456 T13067 T13066 amod significant,differences
R3457 T12979 T12961 prep in,found
R3458 T13068 T13066 prep in,differences
R3459 T13069 T13070 det the,differentiation
R3460 T12980 T12981 det the,ORS
R3461 T13070 T13068 pobj differentiation,in
R3462 T13071 T13070 amod terminal,differentiation
R3463 T13072 T13073 punct (,4A
R3464 T12981 T12979 pobj ORS,in
R3465 T13073 T13066 parataxis 4A,differences
R3466 T13074 T13073 compound Figure,4A
R3467 T13075 T13076 punct –,4D
R3468 T13076 T13073 prep 4D,4A
R3469 T12982 T12981 prep of,ORS
R3470 T13077 T13073 punct ", ",4A
R3471 T13078 T13073 appos 4F,4A
R3472 T12983 T12984 compound hair,follicles
R3473 T13079 T13080 punct –,4I
R3474 T13080 T13078 prep 4I,4F
R3475 T12984 T12982 pobj follicles,of
R3476 T12985 T12981 cc and,ORS
R3477 T13081 T13073 punct ),4A
R3478 T13082 T13039 punct .,showed
R3479 T12986 T12987 amod basal,layer
R3480 T13084 T13085 advmod Similarly,change
R3481 T12987 T12981 conj layer,ORS
R3482 T13086 T13085 punct ", ",change
R3483 T13087 T13088 det the,pattern
R3484 T12988 T12987 prep of,layer
R3485 T13088 T13085 nsubj pattern,change
R3486 T13089 T13088 prep of,pattern
R3487 T13090 T13089 pobj expression,of
R3488 T12989 T12988 pobj epidermis,of
R3489 T13091 T13088 prep of,pattern
R3490 T13092 T13091 pobj K6,of
R3491 T12990 T12981 punct ", ",ORS
R3492 T13093 T13092 punct ", ",K6
R3493 T13094 T13095 dep which,expressed
R3494 T12991 T12992 advmod where,observed
R3495 T13095 T13092 relcl expressed,K6
R3496 T13096 T13095 auxpass is,expressed
R3497 T13097 T13095 advmod normally,expressed
R3498 T13098 T13095 advmod only,expressed
R3499 T13099 T13095 prep in,expressed
R3500 T13100 T13101 det the,layer
R3501 T13101 T13099 pobj layer,in
R3502 T12992 T12981 advcl observed,ORS
R3503 T13102 T13101 amod suprabasal,layer
R3504 T13103 T13102 cc or,suprabasal
R3505 T13104 T13102 conj inner,suprabasal
R3506 T12993 T12994 compound K14,expression
R3507 T13105 T13101 prep of,layer
R3508 T13106 T13107 det the,ORS
R3509 T13107 T13105 pobj ORS,of
R3510 T13108 T13107 prep of,ORS
R3511 T12994 T12992 nsubjpass expression,observed
R3512 T13109 T13110 det the,follicle
R3513 T13110 T13108 pobj follicle,of
R3514 T12995 T12992 auxpass is,observed
R3515 T13111 T13110 compound hair,follicle
R3516 T13112 T13099 cc but,in
R3517 T13113 T13112 neg not,but
R3518 T12996 T12992 advmod also,observed
R3519 T13114 T13099 conj in,in
R3520 T13115 T13116 det the,epidermis
R3521 T13116 T13114 pobj epidermis,in
R3522 T13117 T13118 punct (,4E
R3523 T12997 T12998 punct (,4C
R3524 T13118 T13088 parataxis 4E,pattern
R3525 T13119 T13118 compound Figure,4E
R3526 T13120 T13118 punct ),4E
R3527 T12998 T12961 parataxis 4C,found
R3528 T13121 T13085 punct ", ",change
R3529 T13122 T13085 aux did,change
R3530 T13123 T13085 neg not,change
R3531 T12999 T12998 compound Figure,4C
R3532 T13124 T13085 punct .,change
R3533 T13000 T12998 cc and,4C
R3534 T13126 T13127 prep Due,highlighted
R3535 T13128 T13126 pcomp to,Due
R3536 T13001 T12998 conj 4D,4C
R3537 T13129 T13130 det the,structure
R3538 T13130 T13126 pobj structure,Due
R3539 T13131 T13130 amod abnormal,structure
R3540 T13002 T12998 punct ),4C
R3541 T13132 T13131 cc and,abnormal
R3542 T13133 T13131 conj disorganized,abnormal
R3543 T13003 T12961 punct ", ",found
R3544 T13134 T13130 prep of,structure
R3545 T13135 T13136 compound hair,follicles
R3546 T13136 T13134 pobj follicles,of
R3547 T13004 T13005 mark whereas,observed
R3548 T13137 T13136 appos themselves,follicles
R3549 T13138 T13127 punct ", ",highlighted
R3550 T13139 T13140 compound K6,localization
R3551 T13140 T13127 nsubj localization,highlighted
R3552 T13141 T13142 det the,abnormality
R3553 T13005 T12961 advcl observed,found
R3554 T13142 T13127 dobj abnormality,highlighted
R3555 T13143 T13142 amod histological,abnormality
R3556 T13144 T13145 punct (,4J
R3557 T13006 T13007 det the,expression
R3558 T13007 T13005 nsubjpass expression,observed
R3559 T13008 T13007 prep of,expression
R3560 T13145 T13127 parataxis 4J,highlighted
R3561 T13009 T13008 pobj K1,of
R3562 T13146 T13145 compound Figure,4J
R3563 T13147 T13145 punct ),4J
R3564 T13010 T13009 punct ", ",K1
R3565 T13148 T13127 punct .,highlighted
R3566 T13150 T13151 advmod Yet,seen
R3567 T13011 T13009 conj involucrin,K1
R3568 T13152 T13151 prep as,seen
R3569 T13012 T13011 punct ", ",involucrin
R3570 T13153 T13152 prep in,as
R3571 T13154 T13155 det the,skin
R3572 T13013 T13011 cc and,involucrin
R3573 T13155 T13153 pobj skin,in
R3574 T13156 T13155 amod normal,skin
R3575 T13157 T13151 punct ", ",seen
R3576 T13158 T13151 nsubjpass K6,seen
R3577 T13014 T13011 conj loricrin,involucrin
R3578 T13159 T13151 auxpass was,seen
R3579 T13160 T13151 advmod principally,seen
R3580 T13161 T13162 advmod only,in
R3581 T13015 T13009 punct (,K1
R3582 T13162 T13151 prep in,seen
R3583 T13163 T13164 det the,layer
R3584 T13164 T13162 pobj layer,in
R3585 T13016 T13009 appos markers,K1
R3586 T13165 T13164 amod suprabasal,layer
R3587 T13166 T13164 prep of,layer
R3588 T13167 T13168 det the,ORS
R3589 T13017 T13016 prep for,markers
R3590 T13018 T13019 amod spinous,layers
R3591 T13168 T13166 pobj ORS,of
R3592 T13169 T13170 dep that,colocalize
R3593 T13019 T13017 pobj layers,for
R3594 T13170 T13168 relcl colocalize,ORS
R3595 T13171 T13170 aux did,colocalize
R3596 T13172 T13170 neg not,colocalize
R3597 T13020 T13018 cc and,spinous
R3598 T13173 T13170 prep with,colocalize
R3599 T13174 T13175 det the,markers
R3600 T13175 T13173 pobj markers,with
R3601 T13176 T13175 amod basal,markers
R3602 T13177 T13175 punct ", ",markers
R3603 T13178 T13175 appos K14,markers
R3604 T13021 T13018 conj granular,spinous
R3605 T13179 T13178 cc or,K14
R3606 T13180 T13178 conj K5,K14
R3607 T13181 T13182 punct (,4I
R3608 T13182 T13151 parataxis 4I,seen
R3609 T13022 T13019 prep of,layers
R3610 T13183 T13182 compound Figure,4I
R3611 T13184 T13182 cc and,4I
R3612 T13023 T13022 pobj epidermis,of
R3613 T13185 T13182 conj 4J,4I
R3614 T13186 T13182 punct ),4I
R3615 T13187 T13151 punct .,seen
R3616 T13024 T13005 punct ),observed
R3617 T13189 T13190 prep In,detected
R3618 T13025 T13005 auxpass was,observed
R3619 T13191 T13192 amod normal,skin
R3620 T13192 T13189 pobj skin,In
R3621 T13193 T13190 punct ", ",detected
R3622 T13026 T13005 advmod only,observed
R3623 T13194 T13195 amod proliferating,cells
R3624 T13195 T13190 nsubjpass cells,detected
R3625 T13027 T13005 prep in,observed
R3626 T13196 T13190 auxpass were,detected
R3627 T13197 T13190 prep in,detected
R3628 T13198 T13197 preconj either,in
R3629 T13028 T13029 det the,epidermis
R3630 T13199 T13200 det the,layer
R3631 T13200 T13197 conj layer,in
R3632 T13201 T13200 amod basal,layer
R3633 T13202 T13200 prep of,layer
R3634 T13029 T13027 pobj epidermis,in
R3635 T13203 T13202 pobj epidermis,of
R3636 T13204 T13197 cc or,in
R3637 T13205 T13197 conj in,in
R3638 T13030 T13029 amod nonbasal,epidermis
R3639 T13206 T13207 amod germinative,bulbs
R3640 T13207 T13205 pobj bulbs,in
R3641 T13031 T13032 punct (,data
R3642 T13208 T13209 compound hair,follicle
R3643 T13209 T13207 compound follicle,bulbs
R3644 T13210 T13207 prep at,bulbs
R3645 T13032 T13005 parataxis data,observed
R3646 T13211 T13212 det the,base
R3647 T13212 T13210 pobj base,at
R3648 T13213 T13214 punct (,4B
R3649 T13214 T13190 parataxis 4B,detected
R3650 T13033 T13032 amod unpublished,data
R3651 T13215 T13214 compound Figure,4B
R3652 T13216 T13214 punct ),4B
R3653 T13217 T13190 punct .,detected
R3654 T13219 T13220 prep In,observed
R3655 T13034 T13032 punct ),data
R3656 T13221 T13222 det the,skin
R3657 T13222 T13219 pobj skin,In
R3658 T13223 T13222 amod mutant,skin
R3659 T13035 T12961 punct .,found
R3660 T13224 T13220 punct ", ",observed
R3661 T13225 T13226 preconj either,incorporation
R3662 T13226 T13220 nsubjpass incorporation,observed
R3663 T13227 T13226 compound BrdU,incorporation
R3664 T13037 T13038 det The,patterns
R3665 T13228 T13226 cc or,incorporation
R3666 T13229 T13230 compound Ki67,expression
R3667 T13230 T13226 conj expression,incorporation
R3668 T13231 T13220 auxpass was,observed
R3669 T13038 T13039 nsubj patterns,showed
R3670 T13232 T13233 preconj not,in
R3671 T13233 T13220 prep in,observed
R3672 T13234 T13232 advmod only,not
R3673 T13235 T13233 pobj cells,in
R3674 T13251 T13247 pobj structures,in
R3675 T13236 T13235 prep in,cells
R3676 T13237 T13236 pobj bulbs,in
R3677 T13238 T13237 prep at,bulbs
R3678 T13239 T13240 det the,base
R3679 T13252 T13253 dep that,budding
R3680 T13240 T13238 pobj base,at
R3681 T13241 T13240 prep of,base
R3682 T13242 T13243 det the,follicle
R3683 T13243 T13241 pobj follicle,of
R3684 T13253 T13251 relcl budding,structures
R3685 T13244 T13243 compound hair,follicle
R3686 T13245 T13233 cc but,in
R3687 T13246 T13245 advmod also,but
R3688 T13254 T13253 aux were,budding
R3689 T13247 T13233 conj in,in
R3690 T13248 T13249 npadvmod bulb,like
R3691 T13249 T13251 amod like,structures
R3692 T13255 T13253 prt out,budding
R3693 T13250 T13249 punct -,like
R3694 T13256 T13253 prep from,budding
R3695 T13257 T13258 det the,ORS
R3696 T13258 T13256 pobj ORS,from
R3697 T13259 T13258 prep of,ORS
R3698 T13260 T13261 det the,follicles
R3699 T13261 T13259 pobj follicles,of
R3700 T13357 T13356 prep to,addition
R3701 T13358 T13359 amod diffuse,localization
R3702 T13262 T13261 amod existing,follicles
R3703 T13359 T13357 pobj localization,to
R3704 T13360 T13361 npadvmod membrane,bound
R3705 T13263 T13261 compound hair,follicles
R3706 T13361 T13359 amod bound,localization
R3707 T13362 T13361 punct -,bound
R3708 T13363 T13348 prep as,observed
R3709 T13364 T13363 prep in,as
R3710 T13365 T13366 det the,skin
R3711 T13366 T13364 pobj skin,in
R3712 T13264 T13265 punct (,4G
R3713 T13367 T13366 amod normal,skin
R3714 T13368 T13348 punct ", ",observed
R3715 T13265 T13220 parataxis 4G,observed
R3716 T13369 T13348 nsubjpass cells,observed
R3717 T13370 T13369 prep with,cells
R3718 T13371 T13372 amod strong,localization
R3719 T13266 T13265 compound Figure,4G
R3720 T13372 T13370 pobj localization,with
R3721 T13373 T13374 amod cytosolic,β
R3722 T13374 T13372 nmod β,localization
R3723 T13267 T13265 cc and,4G
R3724 T13375 T13374 punct -,β
R3725 T13376 T13374 appos catenin,β
R3726 T13268 T13265 conj 4G,4G
R3727 T13377 T13348 auxpass were,observed
R3728 T13378 T13348 advmod also,observed
R3729 T13269 T13268 punct ′,4G
R3730 T13379 T13348 advmod frequently,observed
R3731 T13380 T13381 punct (,4H
R3732 T13381 T13348 parataxis 4H,observed
R3733 T13270 T13265 punct ),4G
R3734 T13382 T13381 compound Figure,4H
R3735 T13383 T13381 cc and,4H
R3736 T13271 T13220 punct .,observed
R3737 T13384 T13381 conj 4H,4H
R3738 T13385 T13384 punct ′,4H
R3739 T13386 T13381 punct ),4H
R3740 T13387 T13348 punct .,observed
R3741 T13273 T13274 det Each,tip
R3742 T13389 T13390 det These,cells
R3743 T13390 T13397 nsubjpass cells,surrounded
R3744 T13274 T13276 nsubj tip,becoming
R3745 T13391 T13390 amod elevated,cells
R3746 T13392 T13393 compound β,catenin
R3747 T13393 T13395 npadvmod catenin,expressing
R3748 T13275 T13274 amod budding,tip
R3749 T13394 T13393 punct -,catenin
R3750 T13395 T13390 amod expressing,cells
R3751 T13396 T13395 punct –,expressing
R3752 T13277 T13276 aux was,becoming
R3753 T13398 T13397 auxpass were,surrounded
R3754 T13399 T13397 advmod usually,surrounded
R3755 T13400 T13397 agent by,surrounded
R3756 T13278 T13276 prep like,becoming
R3757 T13401 T13402 amod proliferating,cells
R3758 T13402 T13400 pobj cells,by
R3759 T13403 T13397 punct ", ",surrounded
R3760 T13404 T13397 advcl forming,surrounded
R3761 T13405 T13406 npadvmod bulb,like
R3762 T13279 T13280 det a,bulb
R3763 T13406 T13408 amod like,structures
R3764 T13407 T13406 punct -,like
R3765 T13408 T13404 dobj structures,forming
R3766 T13409 T13397 punct .,surrounded
R3767 T13280 T13278 pobj bulb,like
R3768 T13411 T13412 nsubj Comparison,showed
R3769 T13281 T13282 compound hair,follicle
R3770 T13413 T13411 prep of,Comparison
R3771 T13282 T13280 compound follicle,bulb
R3772 T13414 T13415 advmod immunochemically,stained
R3773 T13415 T13416 amod stained,sections
R3774 T13416 T13413 pobj sections,of
R3775 T13283 T13280 acl containing,bulb
R3776 T13417 T13416 amod serial,sections
R3777 T13418 T13419 mark that,found
R3778 T13284 T13285 amod proliferating,cells
R3779 T13285 T13283 dobj cells,containing
R3780 T13419 T13412 ccomp found,showed
R3781 T13286 T13276 punct .,becoming
R3782 T13420 T13421 det these,stainings
R3783 T13421 T13419 nsubjpass stainings,found
R3784 T13422 T13421 amod intense,stainings
R3785 T13288 T13289 advmod Hence,seems
R3786 T13423 T13424 amod cytosolic,β
R3787 T13424 T13421 nmod β,stainings
R3788 T13425 T13424 punct -,β
R3789 T13290 T13289 punct ", ",seems
R3790 T13426 T13424 appos catenin,β
R3791 T13427 T13419 auxpass were,found
R3792 T13428 T13419 advmod usually,found
R3793 T13429 T13419 prep in,found
R3794 T13291 T13289 prep despite,seems
R3795 T13430 T13431 preconj either,epidermis
R3796 T13431 T13429 pobj epidermis,in
R3797 T13292 T13293 det the,histology
R3798 T13432 T13433 npadvmod K14,positive
R3799 T13433 T13431 amod positive,epidermis
R3800 T13434 T13433 punct -,positive
R3801 T13293 T13291 pobj histology,despite
R3802 T13435 T13436 npadvmod K1,negative
R3803 T13436 T13431 amod negative,epidermis
R3804 T13294 T13293 amod abnormal,histology
R3805 T13437 T13436 punct -,negative
R3806 T13438 T13431 amod basal,epidermis
R3807 T13439 T13431 cc or,epidermis
R3808 T13295 T13293 prep in,histology
R3809 T13440 T13441 npadvmod K14,positive
R3810 T13441 T13443 amod positive,cells
R3811 T13442 T13441 punct -,positive
R3812 T13443 T13431 conj cells,epidermis
R3813 T13444 T13445 npadvmod K6,negative
R3814 T13445 T13443 amod negative,cells
R3815 T13296 T13297 det the,skin
R3816 T13446 T13445 punct -,negative
R3817 T13447 T13443 amod basal,cells
R3818 T13448 T13443 compound ORS,cells
R3819 T13297 T13295 pobj skin,in
R3820 T13449 T13419 punct ", ",found
R3821 T13450 T13419 cc and,found
R3822 T13298 T13297 amod mutant,skin
R3823 T13451 T13452 auxpass are,surrounded
R3824 T13452 T13419 conj surrounded,found
R3825 T13453 T13452 agent by,surrounded
R3826 T13299 T13289 punct ", ",seems
R3827 T13454 T13455 amod proliferating,cells
R3828 T13455 T13453 pobj cells,by
R3829 T13456 T13412 punct .,showed
R3830 T13300 T13289 nsubj proliferation,seems
R3831 T13458 T13459 aux To,determine
R3832 T13301 T13302 aux to,confined
R3833 T13459 T13460 advcl determine,examined
R3834 T13461 T13462 det the,initiation
R3835 T13302 T13289 xcomp confined,seems
R3836 T13303 T13302 auxpass be,confined
R3837 T13304 T13302 prep to,confined
R3838 T13305 T13306 npadvmod bulb,like
R3839 T13462 T13459 dobj initiation,determine
R3840 T13463 T13462 prep of,initiation
R3841 T13306 T13308 amod like,structures
R3842 T13464 T13465 compound hair,follicle
R3843 T13465 T13466 compound follicle,morphogenesis
R3844 T13466 T13463 pobj morphogenesis,of
R3845 T13307 T13306 punct -,like
R3846 T13467 T13459 prep in,determine
R3847 T13468 T13469 det these,mutants
R3848 T13308 T13304 pobj structures,to
R3849 T13469 T13467 pobj mutants,in
R3850 T13470 T13460 punct ", ",examined
R3851 T13471 T13460 nsubj we,examined
R3852 T13309 T13302 prep as,confined
R3853 T13472 T13473 det the,pattern
R3854 T13473 T13460 dobj pattern,examined
R3855 T13310 T13309 prep in,as
R3856 T13474 T13473 compound expression,pattern
R3857 T13475 T13473 prep of,pattern
R3858 T13476 T13477 compound Sonic,hedgehog
R3859 T13311 T13312 det the,skin
R3860 T13477 T13475 pobj hedgehog,of
R3861 T13478 T13477 punct (,hedgehog
R3862 T13479 T13477 appos Shh,hedgehog
R3863 T13480 T13477 punct ),hedgehog
R3864 T13481 T13477 punct ", ",hedgehog
R3865 T13482 T13483 det a,factor
R3866 T13312 T13310 pobj skin,in
R3867 T13483 T13477 appos factor,hedgehog
R3868 T13484 T13483 acl expressed,factor
R3869 T13485 T13484 prep in,expressed
R3870 T13313 T13312 amod normal,skin
R3871 T13486 T13487 compound hair,bulbs
R3872 T13487 T13485 pobj bulbs,in
R3873 T13314 T13315 punct (,4G
R3874 T13488 T13487 prep in,bulbs
R3875 T13489 T13490 amod embryonic,skin
R3876 T13490 T13488 pobj skin,in
R3877 T13315 T13302 parataxis 4G,confined
R3878 T13491 T13492 punct (,Figure
R3879 T13492 T13460 parataxis Figure,examined
R3880 T13316 T13315 compound Figure,4G
R3881 T13493 T13492 nummod 5,Figure
R3882 T13494 T13492 punct ),Figure
R3883 T13495 T13460 punct .,examined
R3884 T13317 T13315 cc and,4G
R3885 T13497 T13498 det The,morphogenesis
R3886 T13318 T13315 conj 4G,4G
R3887 T13498 T13502 nsubj morphogenesis,is
R3888 T13499 T13498 amod aberrant,morphogenesis
R3889 T13500 T13501 compound hair,follicle
R3890 T13319 T13318 punct ′,4G
R3891 T13501 T13498 compound follicle,morphogenesis
R3892 T13503 T13502 acomp evident,is
R3893 T13320 T13315 punct ),4G
R3894 T13504 T13505 advmod as,early
R3895 T13505 T13502 advmod early,is
R3896 T13321 T13289 punct .,seems
R3897 T13506 T13505 prep as,early
R3898 T13507 T13506 pobj E14.5,as
R3899 T13323 T13324 det The,locations
R3900 T13508 T13502 prep in,is
R3901 T13324 T13326 nsubj locations,were
R3902 T13509 T13510 amod mutant,skin
R3903 T13510 T13508 pobj skin,in
R3904 T13511 T13510 amod embryonic,skin
R3905 T13325 T13324 amod exact,locations
R3906 T13512 T13502 punct ", ",is
R3907 T13513 T13502 prep by,is
R3908 T13327 T13324 prep of,locations
R3909 T13514 T13515 amod multiple,expression
R3910 T13515 T13513 pobj expression,by
R3911 T13516 T13515 amod apolarized,expression
R3912 T13517 T13515 prep of,expression
R3913 T13328 T13329 compound hair,follicle
R3914 T13518 T13517 pobj Shh,of
R3915 T13519 T13515 prep throughout,expression
R3916 T13520 T13521 det the,epidermis
R3917 T13521 T13519 pobj epidermis,throughout
R3918 T13522 T13523 punct (,5B
R3919 T13329 T13330 compound follicle,bulbs
R3920 T13523 T13502 parataxis 5B,is
R3921 T13524 T13523 compound Figure,5B
R3922 T13525 T13523 punct ),5B
R3923 T13330 T13327 pobj bulbs,of
R3924 T13526 T13502 punct ", ",is
R3925 T13527 T13528 mark whereas,was
R3926 T13528 T13502 advcl was,is
R3927 T13331 T13326 neg not,were
R3928 T13529 T13528 nsubj that,was
R3929 T13530 T13529 prep of,that
R3930 T13531 T13532 compound control,embryos
R3931 T13332 T13333 advmod as,easy
R3932 T13532 T13530 pobj embryos,of
R3933 T13533 T13534 advmod well,polarized
R3934 T13534 T13528 acomp polarized,was
R3935 T13535 T13534 cc and,polarized
R3936 T13333 T13326 acomp easy,were
R3937 T13536 T13537 advmod regularly,spaced
R3938 T13537 T13534 conj spaced,polarized
R3939 T13538 T13539 punct (,5A
R3940 T13334 T13335 aux to,define
R3941 T13539 T13528 parataxis 5A,was
R3942 T13540 T13539 compound Figure,5A
R3943 T13541 T13539 punct ),5A
R3944 T13335 T13333 advcl define,easy
R3945 T13542 T13502 punct .,is
R3946 T13336 T13326 prep for,were
R3947 T13544 T13545 prep With,invaginate
R3948 T13546 T13544 pobj development,With
R3949 T13337 T13338 det some,follicles
R3950 T13547 T13545 punct ", ",invaginate
R3951 T13548 T13549 compound control,mouse
R3952 T13338 T13336 pobj follicles,for
R3953 T13549 T13550 compound mouse,follicles
R3954 T13550 T13545 nsubj follicles,invaginate
R3955 T13551 T13550 compound hair,follicles
R3956 T13552 T13545 advmod downward,invaginate
R3957 T13339 T13338 amod mutant,follicles
R3958 T13553 T13545 prep in,invaginate
R3959 T13554 T13555 det a,manner
R3960 T13340 T13326 prep due,were
R3961 T13555 T13553 pobj manner,in
R3962 T13556 T13555 amod polarized,manner
R3963 T13557 T13558 punct (,5C
R3964 T13341 T13340 pcomp to,due
R3965 T13558 T13545 parataxis 5C,invaginate
R3966 T13559 T13558 compound Figure,5C
R3967 T13560 T13558 punct ),5C
R3968 T13561 T13545 punct ", ",invaginate
R3969 T13562 T13563 mark whereas,were
R3970 T13563 T13545 advcl were,invaginate
R3971 T13564 T13563 nsubj those,were
R3972 T13342 T13343 poss their,structures
R3973 T13565 T13564 prep of,those
R3974 T13566 T13567 amod mutant,embryos
R3975 T13567 T13565 pobj embryos,of
R3976 T13343 T13340 pobj structures,due
R3977 T13344 T13343 amod disorganized,structures
R3978 T13345 T13326 punct .,were
R3979 T13347 T13348 advmod Interestingly,observed
R3980 T13568 T13569 advmod completely,irregular
R3981 T13349 T13348 punct ", ",observed
R3982 T13569 T13563 acomp irregular,were
R3983 T13570 T13569 cc and,irregular
R3984 T13571 T13569 conj apolarized,irregular
R3985 T13572 T13573 punct (,5D
R3986 T13350 T13348 prep in,observed
R3987 T13573 T13563 parataxis 5D,were
R3988 T13574 T13573 compound Figure,5D
R3989 T13351 T13352 det the,skin
R3990 T13575 T13573 punct ),5D
R3991 T13576 T13545 punct .,invaginate
R3992 T13352 T13350 pobj skin,in
R3993 T13578 T13579 nsubjpass It,noted
R3994 T13353 T13352 amod mutant,skin
R3995 T13580 T13579 auxpass was,noted
R3996 T13581 T13579 advmod also,noted
R3997 T13582 T13583 mark that,was
R3998 T13354 T13348 punct ", ",observed
R3999 T13355 T13348 prep in,observed
R4000 T13583 T13579 ccomp was,noted
R4001 T13356 T13355 pobj addition,in
R4002 T13584 T13585 det the,size
R4003 T13585 T13583 nsubj size,was
R4004 T13586 T13585 prep of,size
R4005 T13674 T13673 punct -,catenin
R4006 T13587 T13588 det each,follicle
R4007 T13588 T13586 pobj follicle,of
R4008 T13589 T13588 punct “,follicle
R4009 T13590 T13588 nmod budding,follicle
R4010 T13675 T13668 prep in,pattern
R4011 T13591 T13588 punct ”,follicle
R4012 T13592 T13585 punct ", ",size
R4013 T13676 T13675 pobj embryos,in
R4014 T13593 T13594 mark as,detected
R4015 T13594 T13585 advcl detected,size
R4016 T13595 T13594 prep by,detected
R4017 T13596 T13597 compound Shh,expression
R4018 T13597 T13595 pobj expression,by
R4019 T13598 T13583 punct ", ",was
R4020 T13677 T13670 advmod clearly,demonstrated
R4021 T13599 T13583 acomp variable,was
R4022 T13600 T13601 punct (,5D
R4023 T13678 T13679 det the,formation
R4024 T13601 T13583 parataxis 5D,was
R4025 T13602 T13601 compound Figure,5D
R4026 T13603 T13601 punct ),5D
R4027 T13679 T13670 dobj formation,demonstrated
R4028 T13604 T13579 punct .,noted
R4029 T13680 T13679 prep of,formation
R4030 T13606 T13607 det The,intensity
R4031 T13607 T13608 nsubj intensity,was
R4032 T13609 T13607 prep of,intensity
R4033 T13610 T13611 compound Shh,staining
R4034 T13611 T13609 pobj staining,of
R4035 T13681 T13682 amod regular,arrays
R4036 T13612 T13608 advmod generally,was
R4037 T13613 T13608 acomp stronger,was
R4038 T13682 T13680 pobj arrays,of
R4039 T13614 T13608 prep in,was
R4040 T13615 T13616 amod mutant,skin
R4041 T13616 T13614 pobj skin,in
R4042 T13683 T13682 prep of,arrays
R4043 T13617 T13608 prep than,was
R4044 T13618 T13617 prep in,than
R4045 T13619 T13620 det the,skin
R4046 T13684 T13685 compound hair,placodes
R4047 T13620 T13618 pobj skin,in
R4048 T13621 T13620 amod normal,skin
R4049 T13622 T13608 punct .,was
R4050 T13685 T13683 pobj placodes,of
R4051 T13624 T13625 det The,initiation
R4052 T13625 T13627 nsubj initiation,was
R4053 T13686 T13670 prep in,demonstrated
R4054 T13626 T13625 amod aberrant,initiation
R4055 T13687 T13688 det the,skin
R4056 T13628 T13625 prep of,initiation
R4057 T13629 T13630 amod multiple,placodes
R4058 T13630 T13628 pobj placodes,of
R4059 T13631 T13630 compound hair,placodes
R4060 T13688 T13686 pobj skin,in
R4061 T13632 T13625 prep during,initiation
R4062 T13633 T13634 amod early,morphogenesis
R4063 T13689 T13688 amod normal,skin
R4064 T13634 T13632 pobj morphogenesis,during
R4065 T13635 T13636 compound hair,follicle
R4066 T13636 T13634 compound follicle,morphogenesis
R4067 T13637 T13627 advmod also,was
R4068 T13638 T13627 acomp evident,was
R4069 T13639 T13627 prep by,was
R4070 T13640 T13641 det the,mount
R4071 T13641 T13639 pobj mount,by
R4072 T13690 T13688 amod embryonic,skin
R4073 T13642 T13641 amod whole,mount
R4074 T13643 T13641 punct -,mount
R4075 T13644 T13645 advmod in,situ
R4076 T13645 T13646 amod situ,hybridization
R4077 T13691 T13692 punct (,5E
R4078 T13646 T13641 appos hybridization,mount
R4079 T13647 T13646 punct (,hybridization
R4080 T13648 T13646 appos ISH,hybridization
R4081 T13649 T13641 punct ),mount
R4082 T13692 T13670 parataxis 5E,demonstrated
R4083 T13650 T13641 prep of,mount
R4084 T13651 T13652 nmod E15.5,embryos
R4085 T13652 T13650 pobj embryos,of
R4086 T13693 T13692 compound Figure,5E
R4087 T13653 T13652 amod mutant,embryos
R4088 T13654 T13641 prep for,mount
R4089 T13694 T13692 cc and,5E
R4090 T13655 T13656 compound β,catenin
R4091 T13656 T13654 pobj catenin,for
R4092 T13657 T13656 punct -,catenin
R4093 T13658 T13659 punct (,5F
R4094 T13695 T13692 conj 5E,5E
R4095 T13659 T13627 parataxis 5F,was
R4096 T13660 T13659 compound Figure,5F
R4097 T13696 T13695 punct ′,5E
R4098 T13661 T13659 cc and,5F
R4099 T13662 T13659 conj 5F,5F
R4100 T13663 T13662 punct ′,5F
R4101 T13664 T13659 punct ),5F
R4102 T13697 T13692 punct ),5E
R4103 T13665 T13627 punct .,was
R4104 T13667 T13668 det The,pattern
R4105 T13698 T13670 punct ", ",demonstrated
R4106 T13668 T13670 nsubj pattern,demonstrated
R4107 T13669 T13668 compound expression,pattern
R4108 T13699 T13670 cc but,demonstrated
R4109 T13671 T13668 prep of,pattern
R4110 T13672 T13673 compound β,catenin
R4111 T13700 T13701 amod such,patterning
R4112 T13673 T13671 pobj catenin,of
R4113 T13701 T13703 nsubjpass patterning,lost
R4114 T13702 T13701 amod regular,patterning
R4115 T13703 T13670 conj lost,demonstrated
R4116 T13704 T13703 auxpass was,lost
R4117 T13705 T13703 punct ", ",lost
R4118 T13780 T13779 compound Figure,5G
R4119 T13706 T13703 cc and,lost
R4120 T13781 T13779 punct ),5G
R4121 T13782 T13764 punct ", ",found
R4122 T13783 T13764 nsubj we,found
R4123 T13784 T13764 advmod also,found
R4124 T13785 T13786 amod ectopic,placodes
R4125 T13707 T13708 advmod often,initiated
R4126 T13786 T13764 dobj placodes,found
R4127 T13787 T13788 advmod irregularly,sized
R4128 T13788 T13786 amod sized,placodes
R4129 T13708 T13703 conj initiated,lost
R4130 T13789 T13788 cc and,sized
R4131 T13790 T13788 conj spaced,sized
R4132 T13791 T13786 compound hair,placodes
R4133 T13709 T13710 advmod tightly,clustered
R4134 T13792 T13764 punct ", ",found
R4135 T13793 T13764 advcl indicating,found
R4136 T13710 T13711 amod clustered,placodes
R4137 T13794 T13795 mark that,have
R4138 T13795 T13793 ccomp have,indicating
R4139 T13796 T13797 det the,footpads
R4140 T13711 T13708 nsubjpass placodes,initiated
R4141 T13797 T13795 nsubj footpads,have
R4142 T13798 T13795 advmod still,have
R4143 T13799 T13800 det the,potential
R4144 T13712 T13711 amod abnormal,placodes
R4145 T13800 T13795 dobj potential,have
R4146 T13801 T13802 aux to,form
R4147 T13713 T13711 compound hair,placodes
R4148 T13802 T13800 acl form,potential
R4149 T13803 T13804 compound hair,placodes
R4150 T13804 T13802 dobj placodes,form
R4151 T13714 T13708 auxpass were,initiated
R4152 T13805 T13802 prep in,form
R4153 T13806 T13807 det the,absence
R4154 T13807 T13805 pobj absence,in
R4155 T13715 T13708 prep in,initiated
R4156 T13808 T13807 prep of,absence
R4157 T13809 T13810 det the,gene
R4158 T13810 T13808 pobj gene,of
R4159 T13716 T13717 amod mutant,embryonic
R4160 T13811 T13810 compound Apc,gene
R4161 T13812 T13813 punct (,5H
R4162 T13813 T13764 parataxis 5H,found
R4163 T13717 T13718 amod embryonic,skin
R4164 T13814 T13813 compound Figure,5H
R4165 T13815 T13813 punct ),5H
R4166 T13718 T13715 pobj skin,in
R4167 T13816 T13764 punct .,found
R4168 T13818 T13819 det These,results
R4169 T13819 T13820 nsubj results,suggest
R4170 T13719 T13720 punct (,5F
R4171 T13821 T13820 advmod collectively,suggest
R4172 T13720 T13708 parataxis 5F,initiated
R4173 T13822 T13823 mark that,take
R4174 T13823 T13820 ccomp take,suggest
R4175 T13824 T13825 det the,differentiation
R4176 T13825 T13823 nsubj differentiation,take
R4177 T13826 T13825 amod terminal,differentiation
R4178 T13827 T13823 aux does,take
R4179 T13721 T13720 compound Figure,5F
R4180 T13828 T13823 dobj place,take
R4181 T13829 T13823 advmod normally,take
R4182 T13830 T13823 prep in,take
R4183 T13831 T13832 det the,skin
R4184 T13722 T13720 punct ′,5F
R4185 T13832 T13830 pobj skin,in
R4186 T13833 T13832 amod mutant,skin
R4187 T13723 T13720 punct ),5F
R4188 T13834 T13823 punct ", ",take
R4189 T13835 T13823 cc but,take
R4190 T13836 T13837 nsubjpass initiation,accompanied
R4191 T13724 T13703 punct .,lost
R4192 T13726 T13727 amod Aberrant,placodes
R4193 T13837 T13823 conj accompanied,take
R4194 T13838 T13836 prep of,initiation
R4195 T13839 T13840 amod embryonic,morphogenesis
R4196 T13840 T13838 pobj morphogenesis,of
R4197 T13727 T13729 nsubj placodes,were
R4198 T13841 T13842 compound hair,follicle
R4199 T13842 T13840 compound follicle,morphogenesis
R4200 T13728 T13727 compound hair,placodes
R4201 T13843 T13837 auxpass is,accompanied
R4202 T13844 T13837 advmod severely,accompanied
R4203 T13845 T13837 aux disrupted,accompanied
R4204 T13846 T13837 punct ", ",accompanied
R4205 T13730 T13729 advmod also,were
R4206 T13847 T13837 agent by,accompanied
R4207 T13848 T13849 det a,morphogenesis
R4208 T13849 T13847 pobj morphogenesis,by
R4209 T13850 T13849 amod continuous,morphogenesis
R4210 T13731 T13729 acomp evident,were
R4211 T13851 T13849 amod ectopic,morphogenesis
R4212 T13852 T13853 compound hair,follicle
R4213 T13732 T13729 prep throughout,were
R4214 T13853 T13849 compound follicle,morphogenesis
R4215 T13854 T13837 prep in,accompanied
R4216 T13855 T13856 amod postnatal,skin
R4217 T13733 T13734 det the,surface
R4218 T13856 T13854 pobj skin,in
R4219 T13857 T13856 amod mutant,skin
R4220 T13734 T13732 pobj surface,throughout
R4221 T13858 T13820 punct .,suggest
R4222 T13735 T13734 compound skin,surface
R4223 T13736 T13734 prep of,surface
R4224 T13737 T13736 pobj limbs,of
R4225 T13738 T13729 prep in,were
R4226 T13739 T13740 compound E15.5,mutants
R4227 T13740 T13738 pobj mutants,in
R4228 T13741 T13742 punct (,5F
R4229 T13742 T13729 parataxis 5F,were
R4230 T13743 T13742 compound Figure,5F
R4231 T13744 T13742 punct ),5F
R4232 T13745 T13729 punct ", ",were
R4233 T13746 T13747 mark whereas,formed
R4234 T13747 T13729 advcl formed,were
R4235 T13748 T13747 nsubj those,formed
R4236 T13749 T13748 prep of,those
R4237 T13750 T13751 det the,embryos
R4238 T13751 T13749 pobj embryos,of
R4239 T13752 T13751 compound control,embryos
R4240 T13753 T13747 aux had,formed
R4241 T13754 T13755 neg not,yet
R4242 T13755 T13747 advmod yet,formed
R4243 T13756 T13757 punct (,5E
R4244 T13757 T13747 parataxis 5E,formed
R4245 T13758 T13757 compound Figure,5E
R4246 T13759 T13757 punct ),5E
R4247 T13760 T13729 punct .,were
R4248 T13762 T13763 advmod Most,interestingly
R4249 T13763 T13764 advmod interestingly,found
R4250 T13765 T13764 punct ", ",found
R4251 T13766 T13764 prep in,found
R4252 T13767 T13768 det the,footpads
R4253 T13768 T13766 pobj footpads,in
R4254 T13769 T13768 amod mutant,footpads
R4255 T13770 T13768 punct ", ",footpads
R4256 T13771 T13772 advmod where,form
R4257 T13772 T13768 relcl form,footpads
R4258 T13773 T13774 compound hair,placodes
R4259 T13774 T13772 nsubj placodes,form
R4260 T13775 T13772 aux do,form
R4261 T13776 T13772 neg not,form
R4262 T13777 T13772 advmod normally,form
R4263 T13778 T13779 punct (,5G
R4264 T13779 T13772 parataxis 5G,form
R4273 T14506 T14505 prep of,Effects
R4274 T14507 T14508 compound K14,cre
R4275 T14508 T14510 npadvmod cre,Driven
R4276 T14509 T14508 punct -,cre
R4277 T14510 T14512 amod Driven,Loss
R4278 T14511 T14510 punct –,Driven
R4279 T14512 T14506 pobj Loss,of
R4280 T14513 T14512 prep of,Loss
R4281 T14514 T14513 pobj Apc,of
R4282 T14515 T14505 prep in,Effects
R4283 T14516 T14517 amod Other,Appendages
R4284 T14517 T14515 pobj Appendages,in
R4285 T14518 T14517 amod Epidermal,Appendages
R4286 T14520 T14521 advcl Similar,affected
R4287 T14522 T14520 prep to,Similar
R4288 T14523 T14524 det the,biology
R4289 T14524 T14522 pobj biology,to
R4290 T14525 T14524 prep of,biology
R4291 T14526 T14527 compound hair,follicles
R4292 T14527 T14525 pobj follicles,of
R4293 T14528 T14521 punct ", ",affected
R4294 T14529 T14530 compound K14,cre
R4295 T14530 T14532 npadvmod cre,driven
R4296 T14531 T14530 punct -,cre
R4297 T14532 T14534 amod driven,loss
R4298 T14533 T14532 punct –,driven
R4299 T14534 T14521 nsubj loss,affected
R4300 T14535 T14534 prep of,loss
R4301 T14536 T14535 pobj Apc,of
R4302 T14537 T14521 advmod also,affected
R4303 T14538 T14539 det the,development
R4304 T14539 T14521 dobj development,affected
R4305 T14540 T14539 prep of,development
R4306 T14541 T14542 amod other,appendages
R4307 T14542 T14540 pobj appendages,of
R4308 T14543 T14542 amod epidermal,appendages
R4309 T14544 T14545 dep that,depend
R4310 T14545 T14542 relcl depend,appendages
R4311 T14546 T14545 prep on,depend
R4312 T14547 T14548 amod epithelial,mesenchymal
R4313 T14548 T14550 amod mesenchymal,interactions
R4314 T14549 T14548 punct –,mesenchymal
R4315 T14550 T14546 pobj interactions,on
R4316 T14551 T14545 prep for,depend
R4317 T14552 T14553 poss their,formation
R4318 T14553 T14551 pobj formation,for
R4319 T14554 T14521 punct .,affected
R4320 T14556 T14557 det The,striking
R4321 T14557 T14559 nsubj striking,was
R4322 T14558 T14557 advmod most,striking
R4323 T14560 T14557 prep of,striking
R4324 T14561 T14560 pobj these,of
R4325 T14562 T14563 amod dental,dysplasia
R4326 T14563 T14559 attr dysplasia,was
R4327 T14564 T14565 punct (,4K
R4328 T14565 T14559 parataxis 4K,was
R4329 T14566 T14565 compound Figure,4K
R4330 T14567 T14568 punct –,4R
R4331 T14568 T14565 prep 4R,4K
R4332 T14569 T14565 punct ),4K
R4333 T14570 T14559 punct .,was
R4334 T14572 T14573 compound Tooth,development
R4335 T14573 T14574 nsubjpass development,initiated
R4336 T14575 T14574 auxpass is,initiated
R4337 T14576 T14574 advmod normally,initiated
R4338 T14577 T14574 prep between,initiated
R4339 T14578 T14577 pobj E11,between
R4340 T14579 T14578 cc and,E11
R4341 T14580 T14578 conj E12,E11
R4342 T14581 T14574 prep by,initiated
R4343 T14582 T14581 pobj invagination,by
R4344 T14583 T14582 prep of,invagination
R4345 T14584 T14585 advmod ectodermally,derived
R4346 T14585 T14586 amod derived,epithelium
R4347 T14586 T14583 pobj epithelium,of
R4348 T14587 T14586 amod oral,epithelium
R4349 T14588 T14582 prep into,invagination
R4350 T14589 T14590 det the,mesenchyme
R4351 T14590 T14588 pobj mesenchyme,into
R4352 T14591 T14590 amod underlying,mesenchyme
R4353 T14592 T14593 amod cranial,crest
R4354 T14593 T14595 npadvmod crest,derived
R4355 T14594 T14593 amod neural,crest
R4356 T14595 T14590 amod derived,mesenchyme
R4357 T14596 T14595 punct –,derived
R4358 T14597 T14574 punct ", ",initiated
R4359 T14598 T14574 advcl generating,initiated
R4360 T14599 T14600 det a,germ
R4361 T14600 T14598 dobj germ,generating
R4362 T14601 T14600 amod tooth,germ
R4363 T14602 T14574 punct .,initiated
R4364 T14604 T14605 prep Despite,revealed
R4365 T14606 T14607 det the,phenotype
R4366 T14607 T14604 pobj phenotype,Despite
R4367 T14608 T14609 advmod grossly,toothless
R4368 T14609 T14607 amod toothless,phenotype
R4369 T14610 T14607 prep of,phenotype
R4370 T14611 T14612 compound KA,mutants
R4371 T14612 T14610 pobj mutants,of
R4372 T14613 T14605 punct ", ",revealed
R4373 T14614 T14615 amod histological,analysis
R4374 T14615 T14605 nsubj analysis,revealed
R4375 T14616 T14615 prep of,analysis
R4376 T14617 T14618 poss their,cavities
R4377 T14618 T14616 pobj cavities,of
R4378 T14619 T14618 amod oral,cavities
R4379 T14620 T14621 det the,formation
R4380 T14621 T14605 dobj formation,revealed
R4381 T14622 T14621 prep of,formation
R4382 T14623 T14624 amod multiple,buds
R4383 T14624 T14622 pobj buds,of
R4384 T14625 T14624 amod tooth,buds
R4385 T14626 T14605 prep at,revealed
R4386 T14627 T14628 det each,location
R4387 T14628 T14626 pobj location,at
R4388 T14629 T14605 punct .,revealed
R4389 T14631 T14632 det These,teeth
R4390 T14632 T14634 nsubj teeth,failed
R4391 T14633 T14632 amod aberrant,teeth
R4392 T14635 T14634 advmod obviously,failed
R4393 T14636 T14637 aux to,grow
R4394 T14637 T14634 xcomp grow,failed
R4395 T14638 T14637 prt out,grow
R4396 T14639 T14637 prep during,grow
R4397 T14640 T14641 det the,transition
R4398 T14641 T14639 pobj transition,during
R4399 T14642 T14641 amod dietary,transition
R4400 T14643 T14641 prep from,transition
R4401 T14644 T14643 pobj milk,from
R4402 T14645 T14641 prep to,transition
R4403 T14646 T14647 amod solid,food
R4404 T14647 T14645 pobj food,to
R4405 T14648 T14634 punct .,failed
R4406 T14650 T14651 advcl Analogous,detected
R4407 T14652 T14650 prep to,Analogous
R4408 T14653 T14654 det the,patterns
R4409 T14654 T14652 pobj patterns,to
R4410 T14655 T14654 compound expression,patterns
R4411 T14656 T14654 prep of,patterns
R4412 T14657 T14656 pobj K14,of
R4413 T14658 T14657 cc and,K14
R4414 T14659 T14660 compound β,catenin
R4415 T14660 T14657 conj catenin,K14
R4416 T14661 T14660 punct -,catenin
R4417 T14662 T14654 prep in,patterns
R4418 T14663 T14664 det the,skin
R4419 T14664 T14662 pobj skin,in
R4420 T14665 T14664 amod normal,skin
R4421 T14666 T14651 punct ", ",detected
R4422 T14667 T14668 amod diffuse,expression
R4423 T14668 T14651 nsubjpass expression,detected
R4424 T14669 T14670 npadvmod membrane,bound
R4425 T14670 T14668 amod bound,expression
R4426 T14671 T14670 punct -,bound
R4427 T14672 T14668 prep of,expression
R4428 T14673 T14674 compound β,catenin
R4429 T14674 T14672 pobj catenin,of
R4430 T14675 T14674 punct -,catenin
R4431 T14676 T14651 auxpass was,detected
R4432 T14677 T14651 prep in,detected
R4433 T14678 T14679 npadvmod K14,expressing
R4434 T14679 T14681 amod expressing,epithelium
R4435 T14680 T14679 punct -,expressing
R4436 T14681 T14677 pobj epithelium,in
R4437 T14682 T14681 amod oral,epithelium
R4438 T14683 T14681 cc and,epithelium
R4439 T14684 T14681 conj ameloblasts,epithelium
R4440 T14685 T14681 prep of,epithelium
R4441 T14686 T14687 amod normal,mice
R4442 T14687 T14685 pobj mice,of
R4443 T14688 T14651 punct .,detected
R4444 T14690 T14691 prep In,showed
R4445 T14692 T14690 pobj mutants,In
R4446 T14693 T14691 punct ", ",showed
R4447 T14694 T14691 nsubj some,showed
R4448 T14695 T14694 prep of,some
R4449 T14696 T14697 det the,cells
R4450 T14697 T14695 pobj cells,of
R4451 T14698 T14699 npadvmod K14,expressing
R4452 T14699 T14697 amod expressing,cells
R4453 T14700 T14699 punct -,expressing
R4454 T14701 T14691 advmod also,showed
R4455 T14702 T14703 amod strong,staining
R4456 T14703 T14691 dobj staining,showed
R4457 T14704 T14705 amod cytosolic,β
R4458 T14705 T14703 nmod β,staining
R4459 T14706 T14705 punct /,β
R4460 T14707 T14705 amod nuclear,β
R4461 T14708 T14705 punct -,β
R4462 T14709 T14705 appos catenin,β
R4463 T14710 T14691 punct ", ",showed
R4464 T14711 T14712 mark as,observed
R4465 T14712 T14691 advcl observed,showed
R4466 T14713 T14712 prep in,observed
R4467 T14714 T14715 det the,skin
R4468 T14715 T14713 pobj skin,in
R4469 T14716 T14715 amod mutant,skin
R4470 T14717 T14718 punct (,4Q
R4471 T14718 T14691 parataxis 4Q,showed
R4472 T14719 T14718 compound Figure,4Q
R4473 T14720 T14718 punct ", ",4Q
R4474 T14721 T14718 conj 4Q,4Q
R4475 T14722 T14721 punct ′,4Q
R4476 T14723 T14721 punct ", ",4Q
R4477 T14724 T14721 cc and,4Q
R4478 T14725 T14721 conj 4R,4Q
R4479 T14726 T14718 punct ),4Q
R4480 T14727 T14691 punct .,showed
R4481 T14729 T14730 nsubj Initiation,was
R4482 T14731 T14729 prep of,Initiation
R4483 T14732 T14733 amod ectopic,buds
R4484 T14733 T14731 pobj buds,of
R4485 T14734 T14733 amod tooth,buds
R4486 T14735 T14729 prep in,Initiation
R4487 T14736 T14737 det the,mice
R4488 T14737 T14735 pobj mice,in
R4489 T14738 T14737 amod mutant,mice
R4490 T14739 T14730 acomp evident,was
R4491 T14740 T14730 prep at,was
R4492 T14741 T14740 pobj E15.5,at
R4493 T14742 T14730 prep by,was
R4494 T14743 T14744 amod extra,dots
R4495 T14744 T14742 pobj dots,by
R4496 T14745 T14744 prep of,dots
R4497 T14746 T14747 compound Shh,expression
R4498 T14747 T14745 pobj expression,of
R4499 T14748 T14744 amod adjacent,dots
R4500 T14749 T14748 prep to,adjacent
R4501 T14750 T14751 det the,teeth
R4502 T14751 T14749 pobj teeth,to
R4503 T14752 T14751 amod primary,teeth
R4504 T14753 T14754 punct (,shown
R4505 T14754 T14730 parataxis shown,was
R4506 T14755 T14754 nsubj data,shown
R4507 T14756 T14754 neg not,shown
R4508 T14757 T14754 punct ),shown
R4509 T14758 T14730 punct .,was
R4510 T14760 T14761 nsubj Loss,leads
R4511 T14762 T14760 prep of,Loss
R4512 T14763 T14762 pobj Apc,of
R4513 T14764 T14761 advmod also,leads
R4514 T14765 T14761 prep to,leads
R4515 T14766 T14765 pobj hyperplasia,to
R4516 T14767 T14766 prep in,hyperplasia
R4517 T14768 T14769 amod squamous,epithelia
R4518 T14769 T14767 pobj epithelia,in
R4519 T14770 T14769 prep of,epithelia
R4520 T14771 T14772 nmod cornea,glands
R4521 T14772 T14770 pobj glands,of
R4522 T14773 T14771 punct ", ",cornea
R4523 T14774 T14771 conj oral,cornea
R4524 T14775 T14774 punct ", ",oral
R4525 T14776 T14774 conj salivary,oral
R4526 T14777 T14776 punct ", ",salivary
R4527 T14778 T14776 cc and,salivary
R4528 T14779 T14776 conj Hardarian,salivary
R4529 T14780 T14781 punct (,data
R4530 T14781 T14761 meta data,leads
R4531 T14782 T14781 amod unpublished,data
R4532 T14783 T14781 punct ),data
R4533 T14784 T14761 punct .,leads
R4534 T14786 T14787 amod Squamous,metaplasia
R4535 T14787 T14788 nsubjpass metaplasia,observed
R4536 T14789 T14787 prep to,metaplasia
R4537 T14790 T14791 compound hair,follicle
R4538 T14791 T14792 npadvmod follicle,like
R4539 T14792 T14794 amod like,structures
R4540 T14793 T14792 punct –,like
R4541 T14794 T14789 pobj structures,to
R4542 T14795 T14787 punct ", ",metaplasia
R4543 T14796 T14797 amod ectopic,morphogenesis
R4544 T14797 T14787 appos morphogenesis,metaplasia
R4545 T14798 T14799 compound hair,follicle
R4546 T14799 T14797 compound follicle,morphogenesis
R4547 T14800 T14788 auxpass was,observed
R4548 T14801 T14788 advmod also,observed
R4549 T14802 T14788 prep in,observed
R4550 T14803 T14804 det these,epithelia
R4551 T14804 T14802 pobj epithelia,in
R4552 T14805 T14788 punct .,observed
R4564 T17241 T17242 compound K14,cre
R4565 T17242 T17244 npadvmod cre,Driven
R4566 T17243 T17242 punct -,cre
R4567 T17244 T17246 amod Driven,Loss
R4568 T17245 T17244 punct –,Driven
R4569 T17246 T17248 nsubj Loss,Results
R4570 T17247 T17246 compound Apc,Loss
R4571 T17249 T17248 prep in,Results
R4572 T17250 T17251 amod Hypoplastic,Athymic
R4573 T17251 T17253 amod Athymic,Mice
R4574 T17252 T17251 punct /,Athymic
R4575 T17253 T17249 pobj Mice,in
R4576 T17255 T17256 nsubj Thymus,is
R4577 T17257 T17258 det an,organ
R4578 T17258 T17256 attr organ,is
R4579 T17259 T17260 dep that,known
R4580 T17260 T17258 relcl known,organ
R4581 T17261 T17260 auxpass is,known
R4582 T17262 T17260 advmod also,known
R4583 T17263 T17264 aux to,have
R4584 T17264 T17260 xcomp have,known
R4585 T17265 T17266 compound K14,expression
R4586 T17266 T17264 dobj expression,have
R4587 T17267 T17268 punct [,16
R4588 T17268 T17258 parataxis 16,organ
R4589 T17269 T17268 punct ],16
R4590 T17270 T17256 punct .,is
R4591 T17272 T17273 nsubj It,represents
R4592 T17274 T17275 det the,organ
R4593 T17275 T17273 dobj organ,represents
R4594 T17276 T17275 amod primary,organ
R4595 T17277 T17275 amod lymphoid,organ
R4596 T17278 T17275 prep for,organ
R4597 T17279 T17280 compound thymocyte,development
R4598 T17280 T17278 pobj development,for
R4599 T17281 T17280 cc and,development
R4600 T17282 T17280 conj selection,development
R4601 T17283 T17273 punct .,represents
R4602 T17285 T17286 amod Distinct,population
R4603 T17286 T17287 nsubj population,mediates
R4604 T17288 T17286 prep of,population
R4605 T17289 T17288 pobj TECs,of
R4606 T17290 T17289 prep of,TECs
R4607 T17291 T17290 pobj cortex,of
R4608 T17292 T17291 cc and,cortex
R4609 T17293 T17291 conj medulla,cortex
R4610 T17294 T17287 dobj both,mediates
R4611 T17295 T17294 prep of,both
R4612 T17296 T17297 det these,functions
R4613 T17297 T17295 pobj functions,of
R4614 T17298 T17297 amod critical,functions
R4615 T17299 T17287 punct .,mediates
R4616 T17301 T17302 amod Cortical,subsets
R4617 T17302 T17306 nsubjpass subsets,characterized
R4618 T17303 T17301 cc and,Cortical
R4619 T17304 T17301 conj medullary,Cortical
R4620 T17305 T17302 compound TEC,subsets
R4621 T17307 T17306 auxpass are,characterized
R4622 T17308 T17306 agent by,characterized
R4623 T17309 T17310 amod differential,expression
R4624 T17310 T17308 pobj expression,by
R4625 T17311 T17310 prep of,expression
R4626 T17312 T17313 nummod four,species
R4627 T17313 T17311 pobj species,of
R4628 T17314 T17313 compound keratin,species
R4629 T17315 T17313 punct : ,species
R4630 T17316 T17313 appos K8,species
R4631 T17317 T17316 punct ", ",K8
R4632 T17318 T17316 conj K18,K8
R4633 T17319 T17318 punct ", ",K18
R4634 T17320 T17318 conj K5,K18
R4635 T17321 T17320 punct ", ",K5
R4636 T17322 T17320 cc and,K5
R4637 T17323 T17320 conj K14,K5
R4638 T17324 T17306 punct .,characterized
R4639 T17326 T17327 det The,thymus
R4640 T17327 T17329 nsubj thymus,is
R4641 T17328 T17327 amod normal,thymus
R4642 T17330 T17331 det a,organ
R4643 T17331 T17329 attr organ,is
R4644 T17332 T17331 amod lobulated,organ
R4645 T17333 T17331 amod lymphoid,organ
R4646 T17334 T17329 punct ", ",is
R4647 T17335 T17336 det each,lobule
R4648 T17336 T17337 nsubj lobule,showing
R4649 T17337 T17329 advcl showing,is
R4650 T17338 T17337 advmod clearly,showing
R4651 T17339 T17340 det the,compartments
R4652 T17340 T17337 dobj compartments,showing
R4653 T17341 T17340 nummod two,compartments
R4654 T17342 T17340 amod distinct,compartments
R4655 T17343 T17340 compound TEC,compartments
R4656 T17344 T17340 punct ", ",compartments
R4657 T17345 T17346 det an,cortex
R4658 T17346 T17340 appos cortex,compartments
R4659 T17347 T17346 amod outer,cortex
R4660 T17348 T17346 cc and,cortex
R4661 T17349 T17350 det an,medulla
R4662 T17350 T17346 conj medulla,cortex
R4663 T17351 T17350 amod inner,medulla
R4664 T17352 T17353 punct (,Figure
R4665 T17353 T17329 parataxis Figure,is
R4666 T17354 T17353 nummod 6,Figure
R4667 T17355 T17353 punct ),Figure
R4668 T17356 T17329 punct .,is
R4669 T17358 T17359 expl There,were
R4670 T17360 T17361 det no,differences
R4671 T17361 T17359 attr differences,were
R4672 T17362 T17361 amod major,differences
R4673 T17363 T17361 prep in,differences
R4674 T17364 T17365 det the,histology
R4675 T17365 T17363 pobj histology,in
R4676 T17366 T17365 prep of,histology
R4677 T17367 T17366 pobj thymus,of
R4678 T17368 T17359 prep between,were
R4679 T17369 T17370 det the,ages
R4680 T17370 T17368 pobj ages,between
R4681 T17371 T17370 appos P3,ages
R4682 T17372 T17371 prep to,P3
R4683 T17373 T17372 pobj P17,to
R4684 T17374 T17359 prep in,were
R4685 T17375 T17376 advmod phenotypically,normal
R4686 T17376 T17377 amod normal,littermates
R4687 T17377 T17374 pobj littermates,in
R4688 T17378 T17359 punct .,were
R4689 T17380 T17381 mark As,shown
R4690 T17381 T17382 advcl shown,formed
R4691 T17383 T17381 prep in,shown
R4692 T17384 T17385 det the,staining
R4693 T17385 T17383 pobj staining,in
R4694 T17386 T17385 nmod H,staining
R4695 T17387 T17386 cc &,H
R4696 T17388 T17386 conj E,H
R4697 T17389 T17385 prep of,staining
R4698 T17390 T17389 pobj thymus,of
R4699 T17391 T17382 punct ", ",formed
R4700 T17392 T17393 det the,cortex
R4701 T17393 T17382 nsubjpass cortex,formed
R4702 T17394 T17382 auxpass was,formed
R4703 T17395 T17382 prep of,formed
R4704 T17396 T17397 amod dense,tissue
R4705 T17397 T17395 pobj tissue,of
R4706 T17398 T17397 amod lymphoid,tissue
R4707 T17399 T17400 dep that,lacks
R4708 T17400 T17397 relcl lacks,tissue
R4709 T17401 T17400 dobj nodules,lacks
R4710 T17402 T17403 punct (,6A
R4711 T17403 T17382 parataxis 6A,formed
R4712 T17404 T17403 compound Figure,6A
R4713 T17405 T17403 punct ),6A
R4714 T17406 T17382 punct .,formed
R4715 T17408 T17409 mark Since,is
R4716 T17409 T17415 advcl is,stained
R4717 T17410 T17411 det the,stroma
R4718 T17411 T17409 nsubj stroma,is
R4719 T17412 T17411 prep of,stroma
R4720 T17413 T17414 det the,medulla
R4721 T17414 T17412 pobj medulla,of
R4722 T17416 T17417 advmod less,heavily
R4723 T17417 T17418 advmod heavily,infiltrated
R4724 T17418 T17409 acomp infiltrated,is
R4725 T17419 T17418 prep with,infiltrated
R4726 T17420 T17419 pobj lymphocytes,with
R4727 T17421 T17418 prep than,infiltrated
R4728 T17422 T17423 det the,cortex
R4729 T17423 T17421 pobj cortex,than
R4730 T17424 T17415 punct ", ",stained
R4731 T17425 T17426 det the,medulla
R4732 T17426 T17415 nsubj medulla,stained
R4733 T17427 T17428 advmod more,lightly
R4734 T17428 T17415 advmod lightly,stained
R4735 T17429 T17428 prep than,lightly
R4736 T17430 T17431 det the,cortex
R4737 T17431 T17429 pobj cortex,than
R4738 T17432 T17415 punct .,stained
R4739 T17434 T17435 prep In,retains
R4740 T17436 T17437 amod normal,mice
R4741 T17437 T17434 pobj mice,In
R4742 T17438 T17435 punct ", ",retains
R4743 T17439 T17440 det the,thymus
R4744 T17440 T17435 nsubj thymus,retains
R4745 T17441 T17442 poss its,size
R4746 T17442 T17435 dobj size,retains
R4747 T17443 T17435 prep until,retains
R4748 T17444 T17445 det the,age
R4749 T17445 T17443 pobj age,until
R4750 T17446 T17447 amod young,adult
R4751 T17447 T17445 nmod adult,age
R4752 T17448 T17435 cc and,retains
R4753 T17449 T17435 conj regresses,retains
R4754 T17450 T17449 advmod thereafter,regresses
R4755 T17451 T17449 prep by,regresses
R4756 T17452 T17451 pobj atrophy,by
R4757 T17453 T17435 punct .,retains
R4758 T17455 T17456 prep In,is
R4759 T17457 T17458 det the,mice
R4760 T17458 T17455 pobj mice,In
R4761 T17459 T17458 amod normal,mice
R4762 T17460 T17458 amod young,mice
R4763 T17461 T17462 nsubj we,examined
R4764 T17462 T17458 advcl examined,mice
R4765 T17463 T17464 punct (,P3
R4766 T17464 T17458 parataxis P3,mice
R4767 T17465 T17466 punct –,P17
R4768 T17466 T17464 prep P17,P3
R4769 T17467 T17464 punct ),P3
R4770 T17468 T17456 punct ", ",is
R4771 T17469 T17456 nsubj it,is
R4772 T17470 T17456 acomp evident,is
R4773 T17471 T17472 mark that,were
R4774 T17472 T17456 ccomp were,is
R4775 T17473 T17472 nsubj thymocytes,were
R4776 T17474 T17475 advmod mitotically,active
R4777 T17475 T17472 acomp active,were
R4778 T17476 T17472 prep in,were
R4779 T17477 T17478 det the,cortex
R4780 T17478 T17476 pobj cortex,in
R4781 T17479 T17480 mark as,determined
R4782 T17480 T17472 advcl determined,were
R4783 T17481 T17480 prep by,determined
R4784 T17482 T17483 compound BrdU,immunostaining
R4785 T17483 T17481 pobj immunostaining,by
R4786 T17484 T17485 punct (,6B
R4787 T17485 T17456 parataxis 6B,is
R4788 T17486 T17485 compound Figure,6B
R4789 T17487 T17485 punct ),6B
R4790 T17488 T17456 punct .,is
R4791 T17490 T17491 nsubj Immunohistochemistry,showed
R4792 T17492 T17490 prep of,Immunohistochemistry
R4793 T17493 T17494 amod normal,thymus
R4794 T17494 T17492 pobj thymus,of
R4795 T17495 T17494 prep from,thymus
R4796 T17496 T17497 nmod P3,mice
R4797 T17497 T17495 pobj mice,from
R4798 T17498 T17496 prep to,P3
R4799 T17499 T17498 pobj P17,to
R4800 T17500 T17501 det a,pattern
R4801 T17501 T17491 dobj pattern,showed
R4802 T17502 T17501 amod similar,pattern
R4803 T17503 T17501 compound staining,pattern
R4804 T17504 T17501 prep for,pattern
R4805 T17505 T17504 pobj K14,for
R4806 T17506 T17507 mark in,restricted
R4807 T17507 T17491 advcl restricted,showed
R4808 T17508 T17507 mark that,restricted
R4809 T17509 T17510 poss its,expression
R4810 T17510 T17507 nsubjpass expression,restricted
R4811 T17511 T17507 auxpass was,restricted
R4812 T17512 T17507 prep to,restricted
R4813 T17513 T17514 det a,population
R4814 T17514 T17512 pobj population,to
R4815 T17515 T17514 amod small,population
R4816 T17516 T17514 prep of,population
R4817 T17517 T17516 pobj TECs,of
R4818 T17518 T17514 prep in,population
R4819 T17519 T17520 det the,region
R4820 T17520 T17518 pobj region,in
R4821 T17521 T17520 amod inner,region
R4822 T17522 T17520 amod medullary,region
R4823 T17523 T17518 cc and,in
R4824 T17524 T17518 conj in,in
R4825 T17525 T17526 det the,keratinocytes
R4826 T17526 T17524 pobj keratinocytes,in
R4827 T17527 T17526 prep in,keratinocytes
R4828 T17528 T17529 poss Hassall,corpuscles
R4829 T17529 T17527 pobj corpuscles,in
R4830 T17530 T17528 case 's,Hassall
R4831 T17531 T17532 punct (,6D
R4832 T17532 T17491 parataxis 6D,showed
R4833 T17533 T17532 compound Figure,6D
R4834 T17534 T17532 punct ),6D
R4835 T17535 T17491 punct .,showed
R4836 T17537 T17538 amod Diffuse,staining
R4837 T17538 T17540 nsubjpass staining,detected
R4838 T17539 T17538 amod cytoplasmic,staining
R4839 T17541 T17538 prep for,staining
R4840 T17542 T17543 compound β,catenin
R4841 T17543 T17541 pobj catenin,for
R4842 T17544 T17543 punct -,catenin
R4843 T17545 T17540 auxpass was,detected
R4844 T17546 T17540 advmod also,detected
R4845 T17547 T17540 prep in,detected
R4846 T17548 T17549 det the,cells
R4847 T17549 T17547 pobj cells,in
R4848 T17550 T17549 amod medullary,cells
R4849 T17551 T17549 amod epithelial,cells
R4850 T17552 T17553 punct (,6C
R4851 T17553 T17540 parataxis 6C,detected
R4852 T17554 T17553 compound Figure,6C
R4853 T17555 T17553 punct ),6C
R4854 T17556 T17540 punct .,detected
R4855 T17558 T17559 prep In,observed
R4856 T17560 T17558 pobj contrast,In
R4857 T17561 T17560 prep to,contrast
R4858 T17562 T17563 compound K14,expression
R4859 T17563 T17561 pobj expression,to
R4860 T17564 T17559 punct ", ",observed
R4861 T17565 T17566 amod diffuse,staining
R4862 T17566 T17559 nsubjpass staining,observed
R4863 T17567 T17566 prep for,staining
R4864 T17568 T17567 pobj K8,for
R4865 T17569 T17559 auxpass was,observed
R4866 T17570 T17559 prep in,observed
R4867 T17571 T17572 amod epithelial,cells
R4868 T17572 T17570 pobj cells,in
R4869 T17573 T17574 preconj both,in
R4870 T17574 T17559 prep in,observed
R4871 T17575 T17576 det the,medulla
R4872 T17576 T17574 pobj medulla,in
R4873 T17577 T17576 cc and,medulla
R4874 T17578 T17576 conj cortex,medulla
R4875 T17579 T17580 punct (,S3
R4876 T17580 T17559 parataxis S3,observed
R4877 T17581 T17580 compound Figure,S3
R4878 T17582 T17580 punct ),S3
R4879 T17583 T17559 punct .,observed
R4880 T17585 T17586 compound K1,staining
R4881 T17586 T17587 nsubjpass staining,detected
R4882 T17588 T17587 auxpass was,detected
R4883 T17589 T17587 neg not,detected
R4884 T17590 T17587 prep in,detected
R4885 T17591 T17592 amod young,mice
R4886 T17592 T17590 pobj mice,in
R4887 T17593 T17587 prep at,detected
R4888 T17594 T17593 pobj P3,at
R4889 T17595 T17587 cc but,detected
R4890 T17596 T17597 prep in,detected
R4891 T17597 T17587 conj detected,detected
R4892 T17598 T17599 amod older,mice
R4893 T17599 T17596 pobj mice,in
R4894 T17600 T17597 nsubjpass it,detected
R4895 T17601 T17597 auxpass was,detected
R4896 T17602 T17597 prep in,detected
R4897 T17603 T17604 amod differentiated,keratinocytes
R4898 T17604 T17602 pobj keratinocytes,in
R4899 T17605 T17597 prep in,detected
R4900 T17606 T17605 pobj some,in
R4901 T17607 T17606 prep of,some
R4902 T17608 T17609 poss Hassall,corpuscles
R4903 T17609 T17607 pobj corpuscles,of
R4904 T17610 T17608 case 's,Hassall
R4905 T17611 T17612 punct (,S3
R4906 T17612 T17597 parataxis S3,detected
R4907 T17613 T17612 compound Figure,S3
R4908 T17614 T17612 punct ),S3
R4909 T17615 T17597 punct .,detected
R4910 T17617 T17618 det The,abnormalities
R4911 T17618 T17620 nsubj abnormalities,were
R4912 T17619 T17618 amod histological,abnormalities
R4913 T17621 T17618 prep of,abnormalities
R4914 T17622 T17621 pobj thymus,of
R4915 T17623 T17620 acomp evident,were
R4916 T17624 T17625 advmod as,early
R4917 T17625 T17620 advmod early,were
R4918 T17626 T17625 prep as,early
R4919 T17627 T17626 pobj P3,as
R4920 T17628 T17620 prep in,were
R4921 T17629 T17630 compound KA,mutants
R4922 T17630 T17628 pobj mutants,in
R4923 T17631 T17632 punct (,6E
R4924 T17632 T17620 parataxis 6E,were
R4925 T17633 T17632 compound Figure,6E
R4926 T17634 T17632 cc and,6E
R4927 T17635 T17632 conj 6H,6E
R4928 T17636 T17632 punct ),6E
R4929 T17637 T17620 punct .,were
R4930 T17639 T17640 det The,thymus
R4931 T17640 T17641 nsubjpass thymus,made
R4932 T17642 T17641 auxpass was,made
R4933 T17643 T17641 prep of,made
R4934 T17644 T17645 nummod two,lobules
R4935 T17645 T17643 pobj lobules,of
R4936 T17646 T17641 prep as,made
R4937 T17647 T17646 prep in,as
R4938 T17648 T17649 det the,mice
R4939 T17649 T17647 pobj mice,in
R4940 T17650 T17649 amod normal,mice
R4941 T17651 T17641 cc but,made
R4942 T17652 T17653 det the,thymus
R4943 T17653 T17655 nsubj thymus,was
R4944 T17654 T17653 amod mutant,thymus
R4945 T17655 T17641 conj was,made
R4946 T17656 T17657 advmod significantly,smaller
R4947 T17657 T17655 acomp smaller,was
R4948 T17658 T17657 prep in,smaller
R4949 T17659 T17658 pobj size,in
R4950 T17660 T17657 prep than,smaller
R4951 T17661 T17660 pobj that,than
R4952 T17662 T17661 prep of,that
R4953 T17663 T17664 det the,controls
R4954 T17664 T17662 pobj controls,of
R4955 T17665 T17666 npadvmod age,matched
R4956 T17666 T17664 amod matched,controls
R4957 T17667 T17666 punct -,matched
R4958 T17668 T17669 punct (,2G
R4959 T17669 T17655 parataxis 2G,was
R4960 T17670 T17669 compound Figure,2G
R4961 T17671 T17669 punct ),2G
R4962 T17672 T17655 punct .,was
R4963 T17674 T17675 advmod Interestingly,reflected
R4964 T17676 T17675 punct ", ",reflected
R4965 T17677 T17675 nsubjpass variations,reflected
R4966 T17678 T17677 prep in,variations
R4967 T17679 T17680 det the,severity
R4968 T17680 T17678 pobj severity,in
R4969 T17681 T17680 amod phenotypic,severity
R4970 T17682 T17680 prep of,severity
R4971 T17683 T17684 det the,pups
R4972 T17684 T17682 pobj pups,of
R4973 T17685 T17684 amod mutant,pups
R4974 T17686 T17677 prep at,variations
R4975 T17687 T17686 pobj P3,at
R4976 T17688 T17675 auxpass were,reflected
R4977 T17689 T17675 advmod prominently,reflected
R4978 T17690 T17675 prep in,reflected
R4979 T17691 T17692 det the,extent
R4980 T17692 T17690 pobj extent,in
R4981 T17693 T17692 prep of,extent
R4982 T17694 T17695 amod histological,abnormalities
R4983 T17695 T17693 pobj abnormalities,of
R4984 T17696 T17695 prep of,abnormalities
R4985 T17697 T17696 pobj thymus,of
R4986 T17698 T17675 punct .,reflected
R4987 T17700 T17701 det A,pup
R4988 T17701 T17705 nsubj pup,showed
R4989 T17702 T17701 nmod P3,pup
R4990 T17703 T17701 nmod KA,pup
R4991 T17704 T17701 amod mutant,pup
R4992 T17706 T17707 dep that,showed
R4993 T17707 T17701 relcl showed,pup
R4994 T17708 T17709 amod milder,phenotype
R4995 T17709 T17707 dobj phenotype,showed
R4996 T17710 T17709 prep with,phenotype
R4997 T17711 T17712 det a,weight
R4998 T17712 T17710 pobj weight,with
R4999 T17713 T17712 amod comparable,weight
R5000 T17714 T17712 compound body,weight
R5001 T17715 T17712 prep to,weight
R5002 T17716 T17717 poss its,littermates
R5003 T17717 T17715 pobj littermates,to
R5004 T17718 T17717 amod normal,littermates
R5005 T17719 T17720 punct (,2A
R5006 T17720 T17707 parataxis 2A,showed
R5007 T17721 T17720 compound Figure,2A
R5008 T17722 T17720 punct ", ",2A
R5009 T17723 T17720 npadvmod M2,2A
R5010 T17724 T17720 punct ),2A
R5011 T17725 T17726 amod milder,abnormalities
R5012 T17726 T17705 dobj abnormalities,showed
R5013 T17727 T17726 compound thymus,abnormalities
R5014 T17728 T17729 punct (,6E
R5015 T17729 T17726 parataxis 6E,abnormalities
R5016 T17730 T17729 compound Figure,6E
R5017 T17731 T17729 punct ),6E
R5018 T17732 T17705 prep compared,showed
R5019 T17733 T17732 prep to,compared
R5020 T17734 T17735 poss its,littermate
R5021 T17735 T17733 pobj littermate,to
R5022 T17736 T17737 advmod more,severe
R5023 T17737 T17735 amod severe,littermate
R5024 T17738 T17735 amod mutant,littermate
R5025 T17739 T17740 punct (,2A
R5026 T17740 T17705 parataxis 2A,showed
R5027 T17741 T17740 compound Figure,2A
R5028 T17742 T17740 punct ", ",2A
R5029 T17743 T17740 npadvmod M1,2A
R5030 T17744 T17740 punct ;,2A
R5031 T17745 T17746 compound Figure,6H
R5032 T17746 T17740 appos 6H,2A
R5033 T17747 T17740 punct ),2A
R5034 T17748 T17705 punct .,showed
R5035 T17750 T17751 det The,thymus
R5036 T17751 T17755 nsubj thymus,was
R5037 T17752 T17751 amod milder,thymus
R5038 T17753 T17751 nmod P3,thymus
R5039 T17754 T17751 amod mutant,thymus
R5040 T17756 T17755 advmod already,was
R5041 T17757 T17758 advmod much,smaller
R5042 T17758 T17755 acomp smaller,was
R5043 T17759 T17758 prep in,smaller
R5044 T17760 T17759 pobj size,in
R5045 T17761 T17755 prep compared,was
R5046 T17762 T17761 prep to,compared
R5047 T17763 T17762 pobj those,to
R5048 T17764 T17763 prep of,those
R5049 T17765 T17766 amod normal,littermates
R5050 T17766 T17764 pobj littermates,of
R5051 T17767 T17768 punct (,shown
R5052 T17768 T17755 parataxis shown,was
R5053 T17769 T17768 nsubj data,shown
R5054 T17770 T17768 neg not,shown
R5055 T17771 T17768 punct ),shown
R5056 T17772 T17755 cc but,was
R5057 T17773 T17774 nummod two,compartments
R5058 T17774 T17776 nsubj compartments,were
R5059 T17775 T17774 amod epithelial,compartments
R5060 T17776 T17755 conj were,was
R5061 T17777 T17774 prep of,compartments
R5062 T17778 T17777 pobj thymus,of
R5063 T17779 T17776 advmod histologically,were
R5064 T17780 T17776 advmod still,were
R5065 T17781 T17776 acomp distinguishable,were
R5066 T17782 T17776 punct ", ",were
R5067 T17783 T17784 mark with,evident
R5068 T17784 T17776 advcl evident,were
R5069 T17785 T17784 nsubj colonization,evident
R5070 T17786 T17785 prep of,colonization
R5071 T17787 T17786 pobj thymocytes,of
R5072 T17788 T17784 prep in,evident
R5073 T17789 T17790 det the,cortex
R5074 T17790 T17788 pobj cortex,in
R5075 T17791 T17776 punct .,were
R5076 T17793 T17794 advmod However,were
R5077 T17795 T17794 punct ", ",were
R5078 T17796 T17794 expl there,were
R5079 T17797 T17798 amod small,populations
R5080 T17798 T17794 attr populations,were
R5081 T17799 T17798 prep of,populations
R5082 T17800 T17801 advmod lightly,stained
R5083 T17801 T17802 amod stained,cells
R5084 T17802 T17799 pobj cells,of
R5085 T17803 T17798 prep by,populations
R5086 T17804 T17803 pobj H,by
R5087 T17805 T17804 cc &,H
R5088 T17806 T17804 conj E,H
R5089 T17807 T17798 acl extending,populations
R5090 T17808 T17807 prep from,extending
R5091 T17809 T17810 det the,edge
R5092 T17810 T17808 pobj edge,from
R5093 T17811 T17810 prep of,edge
R5094 T17812 T17813 det the,cortex
R5095 T17813 T17811 pobj cortex,of
R5096 T17814 T17813 amod outer,cortex
R5097 T17815 T17807 prep towards,extending
R5098 T17816 T17817 amod inner,medulla
R5099 T17817 T17815 pobj medulla,towards
R5100 T17818 T17819 punct (,6E
R5101 T17819 T17794 parataxis 6E,were
R5102 T17820 T17819 compound Figure,6E
R5103 T17821 T17819 punct ),6E
R5104 T17822 T17794 punct ", ",were
R5105 T17823 T17794 cc and,were
R5106 T17824 T17825 det these,cells
R5107 T17825 T17826 nsubj cells,showed
R5108 T17826 T17794 conj showed,were
R5109 T17827 T17828 amod intense,staining
R5110 T17828 T17826 dobj staining,showed
R5111 T17829 T17828 amod nuclear,staining
R5112 T17830 T17831 compound β,catenin
R5113 T17831 T17828 compound catenin,staining
R5114 T17832 T17831 punct -,catenin
R5115 T17833 T17834 mark whereas,showed
R5116 T17834 T17826 advcl showed,showed
R5117 T17835 T17836 det the,rest
R5118 T17836 T17834 nsubj rest,showed
R5119 T17837 T17836 prep of,rest
R5120 T17838 T17839 det the,cells
R5121 T17839 T17837 pobj cells,of
R5122 T17840 T17839 amod medullary,cells
R5123 T17841 T17842 amod diffuse,pattern
R5124 T17842 T17834 dobj pattern,showed
R5125 T17843 T17844 compound β,catenin
R5126 T17844 T17842 compound catenin,pattern
R5127 T17845 T17844 punct -,catenin
R5128 T17846 T17842 compound staining,pattern
R5129 T17847 T17842 amod similar,pattern
R5130 T17848 T17847 prep to,similar
R5131 T17849 T17848 pobj that,to
R5132 T17850 T17849 prep of,that
R5133 T17851 T17852 det the,control
R5134 T17852 T17850 pobj control,of
R5135 T17853 T17854 punct (,6F
R5136 T17854 T17826 parataxis 6F,showed
R5137 T17855 T17854 compound Figure,6F
R5138 T17856 T17854 punct ),6F
R5139 T17857 T17826 punct .,showed
R5140 T17859 T17860 nsubjpass Localization,limited
R5141 T17861 T17859 prep of,Localization
R5142 T17862 T17861 pobj K14,of
R5143 T17863 T17860 auxpass was,limited
R5144 T17864 T17860 prep to,limited
R5145 T17865 T17866 det a,cells
R5146 T17866 T17864 pobj cells,to
R5147 T17867 T17866 amod few,cells
R5148 T17868 T17866 prep in,cells
R5149 T17869 T17870 det the,medulla
R5150 T17870 T17868 pobj medulla,in
R5151 T17871 T17860 cc and,limited
R5152 T17872 T17873 nsubj some,overlapped
R5153 T17873 T17860 conj overlapped,limited
R5154 T17874 T17873 prep with,overlapped
R5155 T17875 T17876 compound K8,localization
R5156 T17876 T17874 pobj localization,with
R5157 T17877 T17878 punct (,6G
R5158 T17878 T17873 parataxis 6G,overlapped
R5159 T17879 T17878 compound Figure,6G
R5160 T17880 T17878 punct ),6G
R5161 T17881 T17873 punct .,overlapped
R5162 T17883 T17884 prep In,lost
R5163 T17885 T17886 det the,thymus
R5164 T17886 T17883 pobj thymus,In
R5165 T17887 T17886 amod other,thymus
R5166 T17888 T17886 nmod P3,thymus
R5167 T17889 T17886 amod mutant,thymus
R5168 T17890 T17891 det the,compartments
R5169 T17891 T17884 nsubjpass compartments,lost
R5170 T17892 T17891 amod distinct,compartments
R5171 T17893 T17894 amod thymic,epithelial
R5172 T17894 T17891 amod epithelial,compartments
R5173 T17895 T17884 aux have,lost
R5174 T17896 T17884 auxpass been,lost
R5175 T17897 T17884 advmod completely,lost
R5176 T17898 T17884 punct ", ",lost
R5177 T17899 T17884 cc and,lost
R5178 T17900 T17901 advmod only,lymphocytes
R5179 T17901 T17904 nsubj lymphocytes,remaining
R5180 T17902 T17901 det a,lymphocytes
R5181 T17903 T17901 amod few,lymphocytes
R5182 T17904 T17884 conj remaining,lost
R5183 T17905 T17904 aux were,remaining
R5184 T17906 T17904 prep at,remaining
R5185 T17907 T17908 det the,edges
R5186 T17908 T17906 pobj edges,at
R5187 T17909 T17904 cc and,remaining
R5188 T17910 T17904 conj some,remaining
R5189 T17911 T17910 prep in,some
R5190 T17912 T17913 det the,middle
R5191 T17913 T17911 pobj middle,in
R5192 T17914 T17915 punct (,6H
R5193 T17915 T17910 parataxis 6H,some
R5194 T17916 T17915 compound Figure,6H
R5195 T17917 T17915 punct ),6H
R5196 T17918 T17904 punct .,remaining
R5197 T17920 T17921 amod Proliferative,activities
R5198 T17921 T17922 nsubjpass activities,observed
R5199 T17923 T17922 auxpass were,observed
R5200 T17924 T17925 neg no,longer
R5201 T17925 T17922 advmod longer,observed
R5202 T17926 T17922 prep in,observed
R5203 T17927 T17926 pobj thymocytes,in
R5204 T17928 T17929 advmod as,prominently
R5205 T17929 T17922 advmod prominently,observed
R5206 T17930 T17929 prep as,prominently
R5207 T17931 T17930 prep in,as
R5208 T17932 T17933 det the,thymus
R5209 T17933 T17931 pobj thymus,in
R5210 T17934 T17933 amod normal,thymus
R5211 T17935 T17922 punct ", ",observed
R5212 T17936 T17922 cc but,observed
R5213 T17937 T17938 det the,cells
R5214 T17938 T17940 nsubj cells,seemed
R5215 T17939 T17938 amod epithelial,cells
R5216 T17940 T17922 conj seemed,observed
R5217 T17941 T17942 aux to,forming
R5218 T17942 T17940 xcomp forming,seemed
R5219 T17943 T17942 aux be,forming
R5220 T17944 T17945 amod concentric,structures
R5221 T17945 T17942 dobj structures,forming
R5222 T17946 T17947 punct (,6I
R5223 T17947 T17942 parataxis 6I,forming
R5224 T17948 T17947 compound Figure,6I
R5225 T17949 T17947 punct ),6I
R5226 T17950 T17940 punct .,seemed
R5227 T17952 T17953 prep Unlike,showed
R5228 T17954 T17952 prep in,Unlike
R5229 T17955 T17956 det the,thymi
R5230 T17956 T17954 pobj thymi,in
R5231 T17957 T17956 amod normal,thymi
R5232 T17958 T17957 cc or,normal
R5233 T17959 T17957 conj mild,normal
R5234 T17960 T17956 amod mutant,thymi
R5235 T17961 T17953 punct ", ",showed
R5236 T17962 T17963 det the,thymus
R5237 T17963 T17953 nsubj thymus,showed
R5238 T17964 T17963 amod severe,thymus
R5239 T17965 T17963 nmod P3,thymus
R5240 T17966 T17963 amod mutant,thymus
R5241 T17967 T17968 amod extensive,expression
R5242 T17968 T17953 dobj expression,showed
R5243 T17969 T17968 compound K14,expression
R5244 T17970 T17971 dep that,overlapped
R5245 T17971 T17968 relcl overlapped,expression
R5246 T17972 T17971 prep with,overlapped
R5247 T17973 T17974 compound K8,expression
R5248 T17974 T17972 pobj expression,with
R5249 T17975 T17976 punct (,6K
R5250 T17976 T17953 parataxis 6K,showed
R5251 T17977 T17976 compound Figure,6K
R5252 T17978 T17976 punct ),6K
R5253 T17979 T17953 punct .,showed
R5254 T17981 T17982 det These,cells
R5255 T17982 T17983 nsubj cells,were
R5256 T17984 T17985 advmod more,like
R5257 T17985 T17983 prep like,were
R5258 T17986 T17987 amod basal,cells
R5259 T17987 T17985 pobj cells,like
R5260 T17988 T17987 prep of,cells
R5261 T17989 T17990 det the,skin
R5262 T17990 T17988 pobj skin,of
R5263 T17991 T17983 prep than,were
R5264 T17992 T17991 pobj TECs,than
R5265 T17993 T17983 cc and,were
R5266 T17994 T17983 conj were,were
R5267 T17995 T17994 acomp adjacent,were
R5268 T17996 T17995 prep to,adjacent
R5269 T17997 T17998 det the,cells
R5270 T17998 T17996 pobj cells,to
R5271 T17999 T18000 advmod most,immature
R5272 T18000 T17998 amod immature,cells
R5273 T18001 T17998 amod looking,cells
R5274 T18002 T18003 dep that,showing
R5275 T18003 T17998 relcl showing,cells
R5276 T18004 T18003 aux were,showing
R5277 T18005 T18006 amod strong,staining
R5278 T18006 T18003 dobj staining,showing
R5279 T18007 T18006 amod nuclear,staining
R5280 T18008 T18007 cc and,nuclear
R5281 T18009 T18007 conj cytoplasmic,nuclear
R5282 T18010 T18011 compound β,catenin
R5283 T18011 T18006 compound catenin,staining
R5284 T18012 T18011 punct -,catenin
R5285 T18013 T18014 punct (,6J
R5286 T18014 T17994 parataxis 6J,were
R5287 T18015 T18014 compound Figure,6J
R5288 T18016 T18014 punct ),6J
R5289 T18017 T17983 punct .,were
R5290 T18019 T18020 det The,staining
R5291 T18020 T18022 nsubjpass staining,observed
R5292 T18021 T18020 amod nuclear,staining
R5293 T18023 T18020 prep of,staining
R5294 T18024 T18025 compound β,catenin
R5295 T18025 T18023 pobj catenin,of
R5296 T18026 T18025 punct -,catenin
R5297 T18027 T18022 auxpass was,observed
R5298 T18028 T18022 neg not,observed
R5299 T18029 T18022 prep in,observed
R5300 T18030 T18031 det the,thymus
R5301 T18031 T18029 pobj thymus,in
R5302 T18032 T18031 amod normal,thymus
R5303 T18033 T18034 npadvmod age,matched
R5304 T18034 T18031 amod matched,thymus
R5305 T18035 T18034 punct -,matched
R5306 T18036 T18037 punct (,6C
R5307 T18037 T18022 parataxis 6C,observed
R5308 T18038 T18037 compound Figure,6C
R5309 T18039 T18037 punct ),6C
R5310 T18040 T18022 punct .,observed
R5311 T18042 T18043 advmod Most,notably
R5312 T18043 T18044 advmod notably,were
R5313 T18045 T18044 punct ", ",were
R5314 T18046 T18047 det the,patterns
R5315 T18047 T18044 nsubj patterns,were
R5316 T18048 T18047 nmod K14,patterns
R5317 T18049 T18048 cc and,K14
R5318 T18050 T18051 compound β,catenin
R5319 T18051 T18048 conj catenin,K14
R5320 T18052 T18051 punct -,catenin
R5321 T18053 T18047 compound staining,patterns
R5322 T18054 T18055 advmod mutually,exclusive
R5323 T18055 T18044 acomp exclusive,were
R5324 T18056 T18057 punct (,6J
R5325 T18057 T18044 parataxis 6J,were
R5326 T18058 T18057 compound Figure,6J
R5327 T18059 T18057 cc and,6J
R5328 T18060 T18057 conj 6K,6J
R5329 T18061 T18057 punct ),6J
R5330 T18062 T18044 punct .,were
R5331 T18064 T18065 prep At,consisted
R5332 T18066 T18064 pobj P10,At
R5333 T18067 T18068 punct –,P13
R5334 T18068 T18066 prep P13,P10
R5335 T18069 T18065 punct ", ",consisted
R5336 T18070 T18071 det the,thymus
R5337 T18071 T18065 nsubj thymus,consisted
R5338 T18072 T18071 amod mutant,thymus
R5339 T18073 T18065 prep of,consisted
R5340 T18074 T18075 amod numerous,structures
R5341 T18075 T18073 pobj structures,of
R5342 T18076 T18075 amod enlarged,structures
R5343 T18077 T18075 poss Hassall,structures
R5344 T18078 T18077 case 's,Hassall
R5345 T18079 T18080 npadvmod corpuscle,like
R5346 T18080 T18075 amod like,structures
R5347 T18081 T18080 punct –,like
R5348 T18082 T18075 punct ", ",structures
R5349 T18083 T18075 acl made,structures
R5350 T18084 T18083 prep of,made
R5351 T18085 T18084 pobj arrays,of
R5352 T18086 T18085 prep of,arrays
R5353 T18087 T18088 npadvmod K14,expressing
R5354 T18088 T18093 amod expressing,cells
R5355 T18089 T18087 punct -,K14
R5356 T18090 T18087 cc and,K14
R5357 T18137 T18138 npadvmod differentiation,specific
R5358 T18091 T18087 conj K8,K14
R5359 T18138 T18140 amod specific,markers
R5360 T18092 T18088 punct -,expressing
R5361 T18139 T18138 punct -,specific
R5362 T18140 T18136 pobj markers,of
R5363 T18093 T18086 pobj cells,of
R5364 T18141 T18140 prep depending,markers
R5365 T18142 T18141 prep on,depending
R5366 T18143 T18144 det the,age
R5367 T18094 T18093 amod keratinizing,cells
R5368 T18144 T18142 pobj age,on
R5369 T18145 T18144 prep of,age
R5370 T18095 T18093 amod epithelial,cells
R5371 T18146 T18145 pobj mice,of
R5372 T18147 T18140 punct ", ",markers
R5373 T18148 T18149 prep in,K1
R5374 T18096 T18093 acl surrounding,cells
R5375 T18149 T18140 appos K1,markers
R5376 T18150 T18151 det this,case
R5377 T18151 T18148 pobj case,in
R5378 T18097 T18098 amod large,deposits
R5379 T18098 T18096 dobj deposits,surrounding
R5380 T18152 T18149 cc and,K1
R5381 T18153 T18149 conj involucrin,K1
R5382 T18154 T18155 dep that,are
R5383 T18155 T18149 relcl are,K1
R5384 T18156 T18155 advmod normally,are
R5385 T18099 T18098 compound keratin,deposits
R5386 T18157 T18155 acomp present,are
R5387 T18158 T18159 advmod only,in
R5388 T18159 T18155 prep in,are
R5389 T18160 T18161 poss Hassall,corpuscles
R5390 T18100 T18101 punct (,6L
R5391 T18161 T18159 pobj corpuscles,in
R5392 T18162 T18160 case 's,Hassall
R5393 T18101 T18065 parataxis 6L,consisted
R5394 T18163 T18135 punct ", ",detected
R5395 T18164 T18135 auxpass were,detected
R5396 T18165 T18135 advmod also,detected
R5397 T18102 T18101 compound Figure,6L
R5398 T18166 T18135 prep in,detected
R5399 T18103 T18101 cc and,6L
R5400 T18167 T18168 amod mutant,thymi
R5401 T18168 T18166 pobj thymi,in
R5402 T18169 T18170 punct (,S3
R5403 T18170 T18135 parataxis S3,detected
R5404 T18171 T18170 compound Figure,S3
R5405 T18104 T18101 conj 6O,6L
R5406 T18172 T18170 punct ),S3
R5407 T18173 T18135 punct .,detected
R5408 T18105 T18101 punct ),6L
R5409 T18175 T18176 prep In,were
R5410 T18106 T18065 punct .,consisted
R5411 T18177 T18178 det these,mice
R5412 T18178 T18175 pobj mice,In
R5413 T18179 T18180 det no,thymocytes
R5414 T18108 T18109 expl There,were
R5415 T18180 T18176 nsubj thymocytes,were
R5416 T18181 T18176 acomp detectable,were
R5417 T18182 T18176 punct .,were
R5418 T18109 T18110 ccomp were,called
R5419 T18184 T18185 compound BrdU,incorporation
R5420 T18185 T18186 nsubjpass incorporation,looking
R5421 T18111 T18112 amod numerous,neutrophils
R5422 T18187 T18186 auxpass was,looking
R5423 T18188 T18186 advmod only,looking
R5424 T18189 T18186 aux observed,looking
R5425 T18190 T18186 prep in,looking
R5426 T18112 T18109 attr neutrophils,were
R5427 T18191 T18192 advmod very,few
R5428 T18192 T18193 amod few,cells
R5429 T18113 T18112 cc and,neutrophils
R5430 T18193 T18190 pobj cells,in
R5431 T18194 T18193 amod keratinizing,cells
R5432 T18195 T18193 amod epithelial,cells
R5433 T18114 T18112 conj macrophages,neutrophils
R5434 T18196 T18186 punct ", ",looking
R5435 T18197 T18198 advmod somewhat,similar
R5436 T18198 T18186 acomp similar,looking
R5437 T18199 T18198 prep to,similar
R5438 T18200 T18201 det the,pattern
R5439 T18201 T18199 pobj pattern,to
R5440 T18115 T18112 acl infiltrating,neutrophils
R5441 T18202 T18201 prep of,pattern
R5442 T18203 T18204 amod mature,skin
R5443 T18204 T18202 pobj skin,of
R5444 T18116 T18117 det the,thymus
R5445 T18205 T18206 punct (,6M
R5446 T18206 T18186 parataxis 6M,looking
R5447 T18207 T18206 compound Figure,6M
R5448 T18208 T18206 punct ),6M
R5449 T18117 T18115 dobj thymus,infiltrating
R5450 T18209 T18186 punct .,looking
R5451 T18118 T18115 prep in,infiltrating
R5452 T18211 T18212 det The,expression
R5453 T18212 T18214 nsubj expression,was
R5454 T18213 T18212 amod diffuse,expression
R5455 T18119 T18118 pobj response,in
R5456 T18215 T18212 prep of,expression
R5457 T18120 T18119 prep to,response
R5458 T18216 T18217 compound β,catenin
R5459 T18217 T18215 pobj catenin,of
R5460 T18218 T18217 punct -,catenin
R5461 T18121 T18122 det these,keratins
R5462 T18219 T18214 advmod also,was
R5463 T18220 T18214 acomp present,was
R5464 T18221 T18214 prep in,was
R5465 T18122 T18120 pobj keratins,to
R5466 T18222 T18223 det these,cells
R5467 T18223 T18221 pobj cells,in
R5468 T18224 T18223 amod epithelial,cells
R5469 T18123 T18110 punct ;,called
R5470 T18225 T18214 punct ", ",was
R5471 T18226 T18214 cc and,was
R5472 T18227 T18228 prep at,were
R5473 T18124 T18110 advmod hence,called
R5474 T18228 T18214 conj were,was
R5475 T18229 T18230 det this,age
R5476 T18230 T18227 pobj age,at
R5477 T18231 T18232 amod fewer,cells
R5478 T18125 T18110 punct ", ",called
R5479 T18232 T18228 nsubj cells,were
R5480 T18233 T18228 acomp positive,were
R5481 T18126 T18127 det these,structures
R5482 T18234 T18233 prep for,positive
R5483 T18235 T18236 amod nuclear,staining
R5484 T18127 T18110 nsubjpass structures,called
R5485 T18236 T18234 pobj staining,for
R5486 T18237 T18238 compound β,catenin
R5487 T18238 T18236 compound catenin,staining
R5488 T18239 T18238 punct -,catenin
R5489 T18128 T18110 aux could,called
R5490 T18240 T18241 punct (,6N
R5491 T18241 T18228 parataxis 6N,were
R5492 T18242 T18241 compound Figure,6N
R5493 T18129 T18110 auxpass be,called
R5494 T18130 T18110 oprd pyogranuloma,called
R5495 T18131 T18110 punct .,called
R5496 T18133 T18134 amod Varying,degrees
R5497 T18243 T18241 punct ),6N
R5498 T18134 T18135 nsubjpass degrees,detected
R5499 T18244 T18228 punct .,were
R5500 T18246 T18247 prep As,observed
R5501 T18136 T18134 prep of,degrees
R5502 T18248 T18246 prep in,As
R5503 T18249 T18250 det the,mice
R5504 T18349 T18346 cc and,loss
R5505 T18250 T18248 pobj mice,in
R5506 T18251 T18250 amod younger,mice
R5507 T18252 T18250 amod mutant,mice
R5508 T18253 T18247 punct ", ",observed
R5509 T18350 T18351 amod consequent,stabilization
R5510 T18254 T18247 advmod however,observed
R5511 T18255 T18247 punct ", ",observed
R5512 T18351 T18346 conj stabilization,loss
R5513 T18256 T18257 amod nuclear,localization
R5514 T18257 T18247 nsubjpass localization,observed
R5515 T18258 T18257 prep of,localization
R5516 T18352 T18351 prep of,stabilization
R5517 T18259 T18260 compound β,catenin
R5518 T18260 T18258 pobj catenin,of
R5519 T18261 T18260 punct -,catenin
R5520 T18353 T18354 compound β,catenin
R5521 T18262 T18247 auxpass was,observed
R5522 T18263 T18247 advmod only,observed
R5523 T18264 T18247 prep in,observed
R5524 T18354 T18352 pobj catenin,of
R5525 T18265 T18266 npadvmod K14,negative
R5526 T18266 T18268 amod negative,cells
R5527 T18267 T18266 punct -,negative
R5528 T18268 T18264 pobj cells,in
R5529 T18355 T18354 punct -,catenin
R5530 T18269 T18270 dep that,looked
R5531 T18270 T18268 relcl looked,cells
R5532 T18356 T18346 prep in,loss
R5533 T18271 T18270 prep like,looked
R5534 T18272 T18273 amod undifferentiated,cells
R5535 T18273 T18271 pobj cells,like
R5536 T18357 T18358 npadvmod K14,expressing
R5537 T18274 T18273 amod basal,cells
R5538 T18275 T18247 punct .,observed
R5539 T18277 T18278 prep In,was
R5540 T18279 T18280 amod older,mice
R5541 T18358 T18360 amod expressing,TECs
R5542 T18280 T18277 pobj mice,In
R5543 T18281 T18280 compound P17,mice
R5544 T18282 T18278 punct ", ",was
R5545 T18359 T18358 punct -,expressing
R5546 T18283 T18284 det the,histopathology
R5547 T18284 T18278 nsubj histopathology,was
R5548 T18285 T18284 cc and,histopathology
R5549 T18360 T18356 pobj TECs,in
R5550 T18286 T18287 compound keratin,pattern
R5551 T18287 T18284 conj pattern,histopathology
R5552 T18361 T18345 prep to,lead
R5553 T18288 T18287 compound expression,pattern
R5554 T18289 T18284 prep of,histopathology
R5555 T18290 T18291 det the,thymus
R5556 T18362 T18363 poss their,proliferation
R5557 T18291 T18289 pobj thymus,of
R5558 T18292 T18291 amod mutant,thymus
R5559 T18293 T18278 acomp similar,was
R5560 T18363 T18361 pobj proliferation,to
R5561 T18294 T18293 prep to,similar
R5562 T18295 T18294 pobj that,to
R5563 T18296 T18295 prep of,that
R5564 T18364 T18363 amod aberrant,proliferation
R5565 T18297 T18296 pobj P13,of
R5566 T18298 T18278 prep except,was
R5567 T18365 T18363 cc and,proliferation
R5568 T18299 T18298 prep for,except
R5569 T18300 T18301 det the,fact
R5570 T18301 T18299 pobj fact,for
R5571 T18302 T18303 mark that,became
R5572 T18366 T18363 conj differentiation,proliferation
R5573 T18303 T18301 acl became,fact
R5574 T18304 T18305 compound β,catenin
R5575 T18367 T18366 prep to,differentiation
R5576 T18305 T18307 compound catenin,expression
R5577 T18306 T18305 punct -,catenin
R5578 T18307 T18303 nsubj expression,became
R5579 T18368 T18367 pobj keratinocytes,to
R5580 T18308 T18309 advmod increasingly,diffuse
R5581 T18309 T18303 acomp diffuse,became
R5582 T18310 T18303 cc and,became
R5583 T18311 T18303 conj appeared,became
R5584 T18369 T18345 punct ", ",lead
R5585 T18312 T18313 aux to,colocalize
R5586 T18313 T18311 xcomp colocalize,appeared
R5587 T18370 T18345 advcl causing,lead
R5588 T18314 T18313 prep with,colocalize
R5589 T18315 T18316 compound K8,K14
R5590 T18316 T18318 compound K14,expression
R5591 T18317 T18316 punct /,K14
R5592 T18318 T18314 pobj expression,with
R5593 T18319 T18320 punct (,shown
R5594 T18371 T18372 amod massive,metaplasia
R5595 T18320 T18278 parataxis shown,was
R5596 T18321 T18320 nsubj data,shown
R5597 T18322 T18320 neg not,shown
R5598 T18372 T18370 dobj metaplasia,causing
R5599 T18373 T18372 amod squamous,metaplasia
R5600 T18323 T18320 punct ),shown
R5601 T18374 T18370 punct ", ",causing
R5602 T18324 T18278 punct .,was
R5603 T18375 T18370 cc rather,causing
R5604 T18326 T18327 nsubj This,coincided
R5605 T18376 T18375 dep than,rather
R5606 T18328 T18327 prep with,coincided
R5607 T18329 T18330 amod fewer,cells
R5608 T18330 T18328 pobj cells,with
R5609 T18377 T18378 aux to,form
R5610 T18331 T18330 amod immature,cells
R5611 T18332 T18330 prep in,cells
R5612 T18378 T18370 conj form,causing
R5613 T18333 T18334 det the,thymus
R5614 T18334 T18332 pobj thymus,in
R5615 T18379 T18380 preconj either,medullary
R5616 T18335 T18334 amod older,thymus
R5617 T18336 T18334 amod mutant,thymus
R5618 T18337 T18327 punct .,coincided
R5619 T18380 T18381 amod medullary,TECs
R5620 T18339 T18340 advmod Collectively,suggest
R5621 T18381 T18378 dobj TECs,form
R5622 T18341 T18340 punct ", ",suggest
R5623 T18342 T18343 det these,results
R5624 T18382 T18380 cc or,medullary
R5625 T18343 T18340 nsubj results,suggest
R5626 T18344 T18345 mark that,lead
R5627 T18383 T18380 conj cortical,medullary
R5628 T18345 T18340 ccomp lead,suggest
R5629 T18346 T18345 nsubj loss,lead
R5630 T18347 T18346 prep of,loss
R5631 T18384 T18340 punct .,suggest
R5632 T18348 T18347 pobj Apc,of
R5633 T18386 T18387 nsubj Loss,resulted
R5634 T18388 T18386 prep of,Loss
R5635 T18389 T18390 amod proper,compartments
R5636 T18390 T18388 pobj compartments,of
R5637 T18391 T18390 compound TEC,compartments
R5638 T18392 T18387 advmod consequently,resulted
R5639 T18393 T18387 prep in,resulted
R5640 T18394 T18393 pobj loss,in
R5641 T18395 T18394 prep of,loss
R5642 T18396 T18395 pobj thymocytes,of
R5643 T18397 T18396 prep for,thymocytes
R5644 T18398 T18397 pobj maturation,for
R5645 T18399 T18394 cc and,loss
R5646 T18400 T18401 det the,mice
R5647 T18401 T18402 nsubj mice,be
R5648 T18402 T18394 conj be,loss
R5649 T18403 T18402 aux to,be
R5650 T18404 T18405 punct “,athymic
R5651 T18405 T18402 acomp athymic,be
R5652 T18406 T18387 punct .,resulted
R5653 T18407 T18387 punct ”,resulted
R918 T4598 T4597 prep of,Generation
R919 T4599 T4600 det the,Mice
R920 T4600 T4598 pobj Mice,of
R921 T4601 T4600 nmod ApcCKO,Mice
R922 T4602 T4601 cc and,ApcCKO
R923 T4603 T4601 conj ApcΔ580,ApcCKO
R924 T4605 T4606 aux To,investigate
R925 T4606 T4607 advcl investigate,used
R926 T4608 T4609 det the,role
R927 T4609 T4606 dobj role,investigate
R928 T4610 T4609 prep of,role
R929 T4611 T4610 pobj Apc,of
R930 T4612 T4609 prep in,role
R931 T4613 T4612 pobj development,in
R932 T4614 T4613 prep of,development
R933 T4615 T4614 pobj skin,of
R934 T4616 T4615 cc and,skin
R935 T4617 T4618 poss its,appendages
R936 T4618 T4615 conj appendages,skin
R937 T4619 T4607 punct ", ",used
R938 T4620 T4607 nsubj we,used
R939 T4621 T4622 det the,technology
R940 T4622 T4607 dobj technology,used
R941 T4623 T4624 compound Cre,loxP
R942 T4624 T4622 compound loxP,technology
R943 T4625 T4624 punct /,loxP
R944 T4626 T4627 aux to,introduce
R945 T4627 T4607 advcl introduce,used
R946 T4628 T4629 det a,mutation
R947 T4629 T4627 dobj mutation,introduce
R948 T4630 T4629 amod conditional,mutation
R949 T4631 T4629 prep of,mutation
R950 T4632 T4633 det the,gene
R951 T4633 T4631 pobj gene,of
R952 T4634 T4633 compound Apc,gene
R953 T4635 T4627 prep in,introduce
R954 T4636 T4635 pobj mice,in
R955 T4637 T4607 punct .,used
R956 T4639 T4640 nsubj We,constructed
R957 T4641 T4642 amod embryonic,stem
R958 T4642 T4643 nmod stem,cells
R959 T4643 T4640 dobj cells,constructed
R960 T4644 T4642 punct (,stem
R961 T4645 T4642 appos ES,stem
R962 T4646 T4643 punct ),cells
R963 T4647 T4643 cc and,cells
R964 T4648 T4643 conj mice,cells
R965 T4649 T4648 acl carrying,mice
R966 T4650 T4651 det an,allele
R967 T4651 T4649 dobj allele,carrying
R968 T4652 T4651 compound Apc,allele
R969 T4653 T4651 acl harboring,allele
R970 T4654 T4655 preconj both,pair
R971 T4655 T4653 dobj pair,harboring
R972 T4656 T4655 det a,pair
R973 T4657 T4655 prep of,pair
R974 T4658 T4659 compound loxP,sites
R975 T4659 T4657 pobj sites,of
R976 T4660 T4655 acl flanking,pair
R977 T4661 T4662 compound Apc,exon
R978 T4662 T4660 dobj exon,flanking
R979 T4663 T4662 nummod 14,exon
R980 T4664 T4655 cc and,pair
R981 T4665 T4666 det a,pair
R982 T4666 T4655 conj pair,pair
R983 T4667 T4666 prep of,pair
R984 T4668 T4669 nmod FLP,target
R985 T4669 T4671 nmod target,sites
R986 T4670 T4669 nmod recognition,target
R987 T4671 T4667 pobj sites,of
R988 T4672 T4669 punct (,target
R989 T4673 T4669 appos FRT,target
R990 T4674 T4671 punct ),sites
R991 T4675 T4666 acl flanking,pair
R992 T4676 T4677 compound PGK,neomycin
R993 T4677 T4679 compound neomycin,cassette
R994 T4678 T4677 punct -,neomycin
R995 T4679 T4675 dobj cassette,flanking
R996 T4680 T4679 compound selection,cassette
R997 T4681 T4640 prep by,constructed
R998 T4682 T4681 pobj recombineering,by
R999 T4683 T4684 punct [,13