PMC:1564426 / 57367-58443 JSONTXT 3 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T30572 0-3 IN denotes For
T30574 4-24 NN denotes immunohistochemistry
T30575 24-26 , denotes ,
T30576 26-27 CD denotes 5
T30578 27-28 HYPH denotes -
T30577 28-30 NNS denotes μm
T30580 31-39 NN denotes paraffin
T30582 39-40 HYPH denotes -
T30581 40-48 VBN denotes embedded
T30583 49-55 NN denotes tissue
T30579 56-64 NNS denotes sections
T30584 65-69 VBD denotes were
T30573 70-83 VBN denotes deparafinized
T30585 84-86 IN denotes in
T30586 87-93 NN denotes xylene
T30587 93-95 , denotes ,
T30588 95-103 VBN denotes followed
T30589 104-106 IN denotes by
T30590 107-114 NN denotes alcohol
T30591 115-126 NN denotes rehydration
T30592 126-127 . denotes .
T30593 127-359 sentence denotes After quenching endogenous peroxidases in 3% H2O2 in methanol, the slides were rinsed in distilled water, and an antigen retrieval step was carried out in a microwave oven for a total of 10 min in preheated citrate buffer (pH 6.0).
T30594 128-133 IN denotes After
T30596 134-143 VBG denotes quenching
T30597 144-154 JJ denotes endogenous
T30598 155-166 NNS denotes peroxidases
T30599 167-169 IN denotes in
T30600 170-171 CD denotes 3
T30601 171-172 NN denotes %
T30602 173-177 NN denotes H2O2
T30603 178-180 IN denotes in
T30604 181-189 NN denotes methanol
T30605 189-191 , denotes ,
T30606 191-194 DT denotes the
T30607 195-201 NNS denotes slides
T30608 202-206 VBD denotes were
T30595 207-213 VBN denotes rinsed
T30609 214-216 IN denotes in
T30610 217-226 JJ denotes distilled
T30611 227-232 NN denotes water
T30612 232-234 , denotes ,
T30613 234-237 CC denotes and
T30614 238-240 DT denotes an
T30616 241-248 NN denotes antigen
T30617 249-258 NN denotes retrieval
T30615 259-263 NN denotes step
T30619 264-267 VBD denotes was
T30618 268-275 VBN denotes carried
T30620 276-279 RP denotes out
T30621 280-282 IN denotes in
T30622 283-284 DT denotes a
T30623 285-294 NN denotes microwave
T30624 295-299 VBN denotes oven
T30625 300-303 IN denotes for
T30626 304-305 DT denotes a
T30627 306-311 NN denotes total
T30628 312-314 IN denotes of
T30629 315-317 CD denotes 10
T30630 318-321 NNS denotes min
T30631 322-324 IN denotes in
T30632 325-334 JJ denotes preheated
T30634 335-342 NN denotes citrate
T30633 343-349 NN denotes buffer
T30635 350-351 -LRB- denotes (
T30636 351-353 NN denotes pH
T30637 354-357 CD denotes 6.0
T30638 357-358 -RRB- denotes )
T30639 358-359 . denotes .
T30640 359-445 sentence denotes The slides were then incubated with primary antibodies at room temperature overnight.
T30641 360-363 DT denotes The
T30642 364-370 NNS denotes slides
T30644 371-375 VBD denotes were
T30645 376-380 RB denotes then
T30643 381-390 VBN denotes incubated
T30646 391-395 IN denotes with
T30647 396-403 JJ denotes primary
T30648 404-414 NNS denotes antibodies
T30649 415-417 IN denotes at
T30650 418-422 NN denotes room
T30651 423-434 NN denotes temperature
T30652 435-444 RB denotes overnight
T30653 444-445 . denotes .
T30654 445-758 sentence denotes Antibodies used were β-catenin (BD Transduction Lab, San Diego, California, United States), keratins 1, 5, 6, 14, involucrin, loricrin (Covance, Berkeley, California, United States), keratin 8 (Abcam, Cambridge, United Kingdom), Ki67 (Vector Laboratories, Burlingame, California, United States) and BrdU (Roche).
T30655 446-456 NNS denotes Antibodies
T30657 457-461 VBN denotes used
T30656 462-466 VBD denotes were
T30658 467-468 NN denotes β
T30660 468-469 HYPH denotes -
T30659 469-476 NN denotes catenin
T30661 477-478 -LRB- denotes (
T30663 478-480 NNP denotes BD
T30664 481-493 NNP denotes Transduction
T30662 494-497 NNP denotes Lab
T30665 497-499 , denotes ,
T30666 499-502 NNP denotes San
T30667 503-508 NNP denotes Diego
T30668 508-510 , denotes ,
T30669 510-520 NNP denotes California
T30670 520-522 , denotes ,
T30671 522-528 NNP denotes United
T30672 529-535 NNP denotes States
T30673 535-536 -RRB- denotes )
T30674 536-538 , denotes ,
T30675 538-546 NNS denotes keratins
T30677 547-548 CD denotes 1
T30678 548-550 , denotes ,
T30679 550-551 CD denotes 5
T30680 551-553 , denotes ,
T30681 553-554 CD denotes 6
T30682 554-556 , denotes ,
T30676 556-558 CD denotes 14
T30683 558-560 , denotes ,
T30684 560-570 NN denotes involucrin
T30685 570-572 , denotes ,
T30686 572-580 NN denotes loricrin
T30687 581-582 -LRB- denotes (
T30688 582-589 NNP denotes Covance
T30689 589-591 , denotes ,
T30690 591-599 NNP denotes Berkeley
T30691 599-601 , denotes ,
T30692 601-611 NNP denotes California
T30693 611-613 , denotes ,
T30694 613-619 NNP denotes United
T30695 620-626 NNP denotes States
T30696 626-627 -RRB- denotes )
T30697 627-629 , denotes ,
T30698 629-636 NN denotes keratin
T30699 637-638 CD denotes 8
T30700 639-640 -LRB- denotes (
T30701 640-645 NNP denotes Abcam
T30702 645-647 , denotes ,
T30703 647-656 NNP denotes Cambridge
T30704 656-658 , denotes ,
T30705 658-664 NNP denotes United
T30706 665-672 NNP denotes Kingdom
T30707 672-673 -RRB- denotes )
T30708 673-675 , denotes ,
T30709 675-679 NN denotes Ki67
T30710 680-681 -LRB- denotes (
T30712 681-687 NNP denotes Vector
T30711 688-700 NNP denotes Laboratories
T30713 700-702 , denotes ,
T30714 702-712 NNP denotes Burlingame
T30715 712-714 , denotes ,
T30716 714-724 NNP denotes California
T30717 724-726 , denotes ,
T30718 726-732 NNP denotes United
T30719 733-739 NNP denotes States
T30720 739-740 -RRB- denotes )
T30721 741-744 CC denotes and
T30722 745-749 NN denotes BrdU
T30723 750-751 -LRB- denotes (
T30724 751-756 NNP denotes Roche
T30725 756-757 -RRB- denotes )
T30726 757-758 . denotes .
T30727 758-998 sentence denotes Biotinylated secondary antibodies (donkey anti-rabbit and goat anti-mouse IgG, 1:250; Jackson ImmunoResearch, West Grove, Pennsylvania, United States), followed by the Vectastain Elite ABC kit (Vector Laboratories) were used for detection.
T30728 759-771 VBN denotes Biotinylated
T30730 772-781 JJ denotes secondary
T30729 782-792 NNS denotes antibodies
T30732 793-794 -LRB- denotes (
T30734 794-800 NN denotes donkey
T30735 801-812 JJ denotes anti-rabbit
T30737 813-816 CC denotes and
T30738 817-821 NN denotes goat
T30739 822-832 JJ denotes anti-mouse
T30736 833-836 NN denotes IgG
T30740 836-838 , denotes ,
T30741 838-839 CD denotes 1
T30742 839-840 SYM denotes :
T30743 840-843 CD denotes 250
T30744 843-844 : denotes ;
T30745 845-852 NNP denotes Jackson
T30733 853-867 NNP denotes ImmunoResearch
T30746 867-869 , denotes ,
T30747 869-873 NNP denotes West
T30748 874-879 NNP denotes Grove
T30749 879-881 , denotes ,
T30750 881-893 NNP denotes Pennsylvania
T30751 893-895 , denotes ,
T30752 895-901 NNP denotes United
T30753 902-908 NNP denotes States
T30754 908-909 -RRB- denotes )
T30755 909-911 , denotes ,
T30756 911-919 VBN denotes followed
T30757 920-922 IN denotes by
T30758 923-926 DT denotes the
T30760 927-937 NNP denotes Vectastain
T30761 938-943 NNP denotes Elite
T30762 944-947 NNP denotes ABC
T30759 948-951 NN denotes kit
T30763 952-953 -LRB- denotes (
T30765 953-959 NNP denotes Vector
T30764 960-972 NNP denotes Laboratories
T30766 972-973 -RRB- denotes )
T30767 974-978 VBD denotes were
T30731 979-983 VBN denotes used
T30768 984-987 IN denotes for
T30769 988-997 NN denotes detection
T30770 997-998 . denotes .
T30771 998-1076 sentence denotes The slides were stained with DAB and counterstained with Mayer's hematoxylin.
T30772 999-1002 DT denotes The
T30773 1003-1009 NNS denotes slides
T30775 1010-1014 VBD denotes were
T30774 1015-1022 VBN denotes stained
T30776 1023-1027 IN denotes with
T30777 1028-1031 NN denotes DAB
T30778 1032-1035 CC denotes and
T30779 1036-1050 VBN denotes counterstained
T30780 1051-1055 IN denotes with
T30781 1056-1061 NNP denotes Mayer
T30783 1061-1063 POS denotes 's
T30782 1064-1075 NN denotes hematoxylin
T30784 1075-1076 . denotes .
R9260 T30572 T30573 prep For,deparafinized
R9261 T30574 T30572 pobj immunohistochemistry,For
R9262 T30575 T30573 punct ", ",deparafinized
R9263 T30576 T30577 nummod 5,μm
R9264 T30577 T30579 nmod μm,sections
R9265 T30578 T30577 punct -,μm
R9266 T30579 T30573 nsubjpass sections,deparafinized
R9267 T30580 T30581 npadvmod paraffin,embedded
R9268 T30581 T30579 amod embedded,sections
R9269 T30582 T30581 punct -,embedded
R9270 T30583 T30579 compound tissue,sections
R9271 T30584 T30573 auxpass were,deparafinized
R9272 T30585 T30573 prep in,deparafinized
R9273 T30586 T30585 pobj xylene,in
R9274 T30587 T30573 punct ", ",deparafinized
R9275 T30588 T30573 advcl followed,deparafinized
R9276 T30589 T30588 agent by,followed
R9277 T30590 T30591 compound alcohol,rehydration
R9278 T30591 T30589 pobj rehydration,by
R9279 T30592 T30573 punct .,deparafinized
R9280 T30594 T30595 prep After,rinsed
R9281 T30596 T30594 pcomp quenching,After
R9282 T30597 T30598 amod endogenous,peroxidases
R9283 T30598 T30596 dobj peroxidases,quenching
R9284 T30599 T30596 prep in,quenching
R9285 T30600 T30601 nummod 3,%
R9286 T30601 T30602 compound %,H2O2
R9287 T30602 T30599 pobj H2O2,in
R9288 T30603 T30596 prep in,quenching
R9289 T30604 T30603 pobj methanol,in
R9290 T30605 T30595 punct ", ",rinsed
R9291 T30606 T30607 det the,slides
R9292 T30607 T30595 nsubjpass slides,rinsed
R9293 T30608 T30595 auxpass were,rinsed
R9294 T30609 T30595 prep in,rinsed
R9295 T30610 T30611 amod distilled,water
R9296 T30611 T30609 pobj water,in
R9297 T30612 T30595 punct ", ",rinsed
R9298 T30613 T30595 cc and,rinsed
R9299 T30614 T30615 det an,step
R9300 T30615 T30618 nsubjpass step,carried
R9301 T30616 T30615 compound antigen,step
R9302 T30617 T30615 compound retrieval,step
R9303 T30618 T30595 conj carried,rinsed
R9304 T30619 T30618 auxpass was,carried
R9305 T30620 T30618 prt out,carried
R9306 T30621 T30618 prep in,carried
R9307 T30622 T30623 det a,microwave
R9308 T30623 T30621 pobj microwave,in
R9309 T30624 T30623 amod oven,microwave
R9310 T30625 T30618 prep for,carried
R9311 T30626 T30627 det a,total
R9312 T30627 T30625 pobj total,for
R9313 T30628 T30627 prep of,total
R9314 T30629 T30630 nummod 10,min
R9315 T30630 T30628 pobj min,of
R9316 T30631 T30618 prep in,carried
R9317 T30632 T30633 amod preheated,buffer
R9318 T30633 T30631 pobj buffer,in
R9319 T30634 T30633 compound citrate,buffer
R9320 T30635 T30636 punct (,pH
R9321 T30636 T30633 parataxis pH,buffer
R9322 T30637 T30636 nummod 6.0,pH
R9323 T30638 T30636 punct ),pH
R9324 T30639 T30595 punct .,rinsed
R9325 T30641 T30642 det The,slides
R9326 T30642 T30643 nsubjpass slides,incubated
R9327 T30644 T30643 auxpass were,incubated
R9328 T30645 T30643 advmod then,incubated
R9329 T30646 T30643 prep with,incubated
R9330 T30647 T30648 amod primary,antibodies
R9331 T30648 T30646 pobj antibodies,with
R9332 T30649 T30643 prep at,incubated
R9333 T30650 T30651 compound room,temperature
R9334 T30651 T30649 pobj temperature,at
R9335 T30652 T30643 advmod overnight,incubated
R9336 T30653 T30643 punct .,incubated
R9337 T30655 T30656 nsubj Antibodies,were
R9338 T30657 T30655 acl used,Antibodies
R9339 T30658 T30659 compound β,catenin
R9340 T30659 T30656 attr catenin,were
R9341 T30660 T30659 punct -,catenin
R9342 T30661 T30662 punct (,Lab
R9343 T30662 T30659 parataxis Lab,catenin
R9344 T30663 T30662 compound BD,Lab
R9345 T30664 T30662 compound Transduction,Lab
R9346 T30665 T30662 punct ", ",Lab
R9347 T30666 T30667 compound San,Diego
R9348 T30667 T30662 npadvmod Diego,Lab
R9349 T30668 T30662 punct ", ",Lab
R9350 T30669 T30662 npadvmod California,Lab
R9351 T30670 T30662 punct ", ",Lab
R9352 T30671 T30672 compound United,States
R9353 T30672 T30662 npadvmod States,Lab
R9354 T30673 T30662 punct ),Lab
R9355 T30674 T30659 punct ", ",catenin
R9356 T30675 T30676 nmod keratins,14
R9357 T30676 T30659 conj 14,catenin
R9358 T30677 T30676 nummod 1,14
R9359 T30678 T30676 punct ", ",14
R9360 T30679 T30676 nummod 5,14
R9361 T30680 T30676 punct ", ",14
R9362 T30681 T30676 nummod 6,14
R9363 T30682 T30676 punct ", ",14
R9364 T30683 T30676 punct ", ",14
R9365 T30684 T30676 conj involucrin,14
R9366 T30685 T30684 punct ", ",involucrin
R9367 T30686 T30684 conj loricrin,involucrin
R9368 T30687 T30688 punct (,Covance
R9369 T30688 T30686 parataxis Covance,loricrin
R9370 T30689 T30688 punct ", ",Covance
R9371 T30690 T30688 npadvmod Berkeley,Covance
R9372 T30691 T30688 punct ", ",Covance
R9373 T30692 T30688 npadvmod California,Covance
R9374 T30693 T30688 punct ", ",Covance
R9375 T30694 T30695 compound United,States
R9376 T30695 T30688 npadvmod States,Covance
R9377 T30696 T30688 punct ),Covance
R9378 T30697 T30686 punct ", ",loricrin
R9379 T30698 T30686 conj keratin,loricrin
R9380 T30699 T30698 nummod 8,keratin
R9381 T30700 T30701 punct (,Abcam
R9382 T30701 T30698 parataxis Abcam,keratin
R9383 T30702 T30701 punct ", ",Abcam
R9384 T30703 T30701 npadvmod Cambridge,Abcam
R9385 T30704 T30701 punct ", ",Abcam
R9386 T30705 T30706 compound United,Kingdom
R9387 T30706 T30701 npadvmod Kingdom,Abcam
R9388 T30707 T30701 punct ),Abcam
R9389 T30708 T30698 punct ", ",keratin
R9390 T30709 T30698 conj Ki67,keratin
R9391 T30710 T30711 punct (,Laboratories
R9392 T30711 T30709 parataxis Laboratories,Ki67
R9393 T30712 T30711 compound Vector,Laboratories
R9394 T30713 T30711 punct ", ",Laboratories
R9395 T30714 T30711 npadvmod Burlingame,Laboratories
R9396 T30715 T30711 punct ", ",Laboratories
R9397 T30716 T30711 npadvmod California,Laboratories
R9398 T30717 T30711 punct ", ",Laboratories
R9399 T30718 T30719 compound United,States
R9400 T30719 T30711 npadvmod States,Laboratories
R9401 T30720 T30711 punct ),Laboratories
R9402 T30721 T30709 cc and,Ki67
R9403 T30722 T30709 conj BrdU,Ki67
R9404 T30723 T30724 punct (,Roche
R9405 T30724 T30722 parataxis Roche,BrdU
R9406 T30725 T30724 punct ),Roche
R9407 T30726 T30656 punct .,were
R9408 T30728 T30729 amod Biotinylated,antibodies
R9409 T30729 T30731 nsubjpass antibodies,used
R9410 T30730 T30729 amod secondary,antibodies
R9411 T30732 T30733 punct (,ImmunoResearch
R9412 T30733 T30729 parataxis ImmunoResearch,antibodies
R9413 T30734 T30735 npadvmod donkey,anti-rabbit
R9414 T30735 T30736 amod anti-rabbit,IgG
R9415 T30736 T30733 dep IgG,ImmunoResearch
R9416 T30737 T30735 cc and,anti-rabbit
R9417 T30738 T30739 npadvmod goat,anti-mouse
R9418 T30739 T30735 conj anti-mouse,anti-rabbit
R9419 T30740 T30736 punct ", ",IgG
R9420 T30741 T30736 npadvmod 1,IgG
R9421 T30742 T30743 punct :,250
R9422 T30743 T30741 prep 250,1
R9423 T30744 T30733 punct ;,ImmunoResearch
R9424 T30745 T30733 compound Jackson,ImmunoResearch
R9425 T30746 T30733 punct ", ",ImmunoResearch
R9426 T30747 T30748 compound West,Grove
R9427 T30748 T30733 npadvmod Grove,ImmunoResearch
R9428 T30749 T30733 punct ", ",ImmunoResearch
R9429 T30750 T30733 npadvmod Pennsylvania,ImmunoResearch
R9430 T30751 T30733 punct ", ",ImmunoResearch
R9431 T30752 T30753 compound United,States
R9432 T30753 T30733 npadvmod States,ImmunoResearch
R9433 T30754 T30733 punct ),ImmunoResearch
R9434 T30755 T30729 punct ", ",antibodies
R9435 T30756 T30729 acl followed,antibodies
R9436 T30757 T30756 agent by,followed
R9437 T30758 T30759 det the,kit
R9438 T30759 T30757 pobj kit,by
R9439 T30760 T30759 compound Vectastain,kit
R9440 T30761 T30759 compound Elite,kit
R9441 T30762 T30759 compound ABC,kit
R9442 T30763 T30764 punct (,Laboratories
R9443 T30764 T30759 parataxis Laboratories,kit
R9444 T30765 T30764 compound Vector,Laboratories
R9445 T30766 T30764 punct ),Laboratories
R9446 T30767 T30731 auxpass were,used
R9447 T30768 T30731 prep for,used
R9448 T30769 T30768 pobj detection,for
R9449 T30770 T30731 punct .,used
R9450 T30772 T30773 det The,slides
R9451 T30773 T30774 nsubjpass slides,stained
R9452 T30775 T30774 auxpass were,stained
R9453 T30776 T30774 prep with,stained
R9454 T30777 T30776 pobj DAB,with
R9455 T30778 T30774 cc and,stained
R9456 T30779 T30774 conj counterstained,stained
R9457 T30780 T30779 prep with,counterstained
R9458 T30781 T30782 poss Mayer,hematoxylin
R9459 T30782 T30780 pobj hematoxylin,with
R9460 T30783 T30781 case 's,Mayer
R9461 T30784 T30774 punct .,stained