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PMC:1540739 / 8882-10206 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T5752 0-8 NN denotes Exchange
T5753 9-19 NNS denotes constructs
T5754 19-21 : denotes :
T5755 21-27 VBG denotes making
T5756 28-31 DT denotes the
T5757 32-38 NN denotes p53GFP
T5759 39-42 CC denotes and
T5760 43-46 NN denotes p53
T5762 46-47 NN denotes Δ
T5761 47-51 NN denotes PGFP
T5758 52-60 NNS denotes plasmids
T5763 60-284 sentence denotes To make a p53-GFP fusion protein, we first subcloned a SacII-HindIII fragment of the p53 locus (corresponding to part of exon 10 to sequences downstream of the gene) into pBS, then mutated the HindIII site into a FseI site.
T5764 61-63 TO denotes To
T5765 64-68 VB denotes make
T5767 69-70 DT denotes a
T5769 71-74 NN denotes p53
T5771 74-75 HYPH denotes -
T5770 75-78 NN denotes GFP
T5772 79-85 NN denotes fusion
T5768 86-93 NN denotes protein
T5773 93-95 , denotes ,
T5774 95-97 PRP denotes we
T5775 98-103 RB denotes first
T5766 104-113 VBD denotes subcloned
T5776 114-115 DT denotes a
T5778 116-121 NN denotes SacII
T5780 121-122 HYPH denotes -
T5779 122-129 NN denotes HindIII
T5777 130-138 NN denotes fragment
T5781 139-141 IN denotes of
T5782 142-145 DT denotes the
T5784 146-149 NN denotes p53
T5783 150-155 NN denotes locus
T5785 156-157 -LRB- denotes (
T5786 157-170 VBG denotes corresponding
T5787 171-173 IN denotes to
T5788 174-178 NN denotes part
T5789 179-181 IN denotes of
T5790 182-186 NN denotes exon
T5791 187-189 CD denotes 10
T5792 190-192 IN denotes to
T5793 193-202 NNS denotes sequences
T5794 203-213 RB denotes downstream
T5795 214-216 IN denotes of
T5796 217-220 DT denotes the
T5797 221-225 NN denotes gene
T5798 225-226 -RRB- denotes )
T5799 227-231 IN denotes into
T5800 232-235 NN denotes pBS
T5801 235-237 , denotes ,
T5802 237-241 RB denotes then
T5803 242-249 VBD denotes mutated
T5804 250-253 DT denotes the
T5806 254-261 NN denotes HindIII
T5805 262-266 NN denotes site
T5807 267-271 IN denotes into
T5808 272-273 DT denotes a
T5810 274-278 NN denotes FseI
T5809 279-283 NN denotes site
T5811 283-284 . denotes .
T5812 284-675 sentence denotes We next mutated the C-terminal part of the p53 gene in two rounds of PCR mutagenesis, first with primers 5′-GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC-3′ and 5′-GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC-3′, which removed the stop codon and introduced a BamHI site, then with primers 5′-GACGGATCCCCTCTGAATTCCGTCCCCATCACCA-3′ and 5′-TGGTGATGGGGACGGAATACAGAGGGGATCCGTC-3′, which introduced an EcoRI site.
T5813 285-287 PRP denotes We
T5815 288-292 RB denotes next
T5814 293-300 VBD denotes mutated
T5816 301-304 DT denotes the
T5818 305-306 NN denotes C
T5820 306-307 HYPH denotes -
T5819 307-315 JJ denotes terminal
T5817 316-320 NN denotes part
T5821 321-323 IN denotes of
T5822 324-327 DT denotes the
T5824 328-331 NN denotes p53
T5823 332-336 NN denotes gene
T5825 337-339 IN denotes in
T5826 340-343 CD denotes two
T5827 344-350 NNS denotes rounds
T5828 351-353 IN denotes of
T5829 354-357 NN denotes PCR
T5830 358-369 NN denotes mutagenesis
T5831 369-371 , denotes ,
T5832 371-376 RB denotes first
T5833 377-381 IN denotes with
T5834 382-389 NNS denotes primers
T5836 390-391 CD denotes 5
T5837 391-392 SYM denotes ′
T5838 392-393 HYPH denotes -
T5835 393-429 NN denotes GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
T5839 429-430 HYPH denotes -
T5840 430-431 CD denotes 3
T5841 431-432 SYM denotes ′
T5842 433-436 CC denotes and
T5843 437-438 CD denotes 5
T5845 438-439 SYM denotes ′
T5846 439-440 HYPH denotes -
T5844 440-476 NN denotes GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
T5847 476-477 HYPH denotes -
T5848 477-478 CD denotes 3
T5849 478-479 SYM denotes ′
T5850 479-481 , denotes ,
T5851 481-486 WDT denotes which
T5852 487-494 VBD denotes removed
T5853 495-498 DT denotes the
T5855 499-503 NN denotes stop
T5854 504-509 NN denotes codon
T5856 510-513 CC denotes and
T5857 514-524 VBD denotes introduced
T5858 525-526 DT denotes a
T5860 527-532 NN denotes BamHI
T5859 533-537 NN denotes site
T5861 537-539 , denotes ,
T5862 539-543 RB denotes then
T5863 544-548 IN denotes with
T5864 549-556 NNS denotes primers
T5866 557-558 CD denotes 5
T5867 558-559 SYM denotes ′
T5868 559-560 HYPH denotes -
T5865 560-594 NN denotes GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
T5869 594-595 HYPH denotes -
T5870 595-596 CD denotes 3
T5871 596-597 SYM denotes ′
T5872 598-601 CC denotes and
T5873 602-603 CD denotes 5
T5875 603-604 SYM denotes ′
T5876 604-605 HYPH denotes -
T5874 605-639 NN denotes TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
T5877 639-640 HYPH denotes -
T5878 640-641 CD denotes 3
T5879 641-642 SYM denotes ′
T5880 642-644 , denotes ,
T5881 644-649 WDT denotes which
T5882 650-660 VBD denotes introduced
T5883 661-663 DT denotes an
T5885 664-669 NN denotes EcoRI
T5884 670-674 NN denotes site
T5886 674-675 . denotes .
T5887 675-862 sentence denotes We verified the sequence from the mutated plasmid, then digested it with BamHI and EcoRI, to insert in frame GFP sequences from a Bam HI-EcoRI fragment of plasmid phr-GFP-1 (Stratagene).
T5888 676-678 PRP denotes We
T5889 679-687 VBD denotes verified
T5890 688-691 DT denotes the
T5891 692-700 NN denotes sequence
T5892 701-705 IN denotes from
T5893 706-709 DT denotes the
T5895 710-717 VBN denotes mutated
T5894 718-725 NN denotes plasmid
T5896 725-727 , denotes ,
T5897 727-731 RB denotes then
T5898 732-740 VBD denotes digested
T5899 741-743 PRP denotes it
T5900 744-748 IN denotes with
T5901 749-754 NN denotes BamHI
T5902 755-758 CC denotes and
T5903 759-764 NN denotes EcoRI
T5904 764-766 , denotes ,
T5905 766-768 TO denotes to
T5906 769-775 VB denotes insert
T5907 776-778 IN denotes in
T5908 779-784 NN denotes frame
T5909 785-788 NN denotes GFP
T5910 789-798 NNS denotes sequences
T5911 799-803 IN denotes from
T5912 804-805 DT denotes a
T5914 806-809 NN denotes Bam
T5916 810-812 NN denotes HI
T5917 812-813 HYPH denotes -
T5915 813-818 NN denotes EcoRI
T5913 819-827 NN denotes fragment
T5918 828-830 IN denotes of
T5919 831-838 NN denotes plasmid
T5921 839-842 NN denotes phr
T5922 842-843 HYPH denotes -
T5920 843-846 NN denotes GFP
T5923 846-847 HYPH denotes -
T5924 847-848 CD denotes 1
T5925 849-850 -LRB- denotes (
T5926 850-860 NNP denotes Stratagene
T5927 860-861 -RRB- denotes )
T5928 861-862 . denotes .
T5929 862-1105 sentence denotes We verified the sequence of this p53-GFP fusion fragment, then swapped it in the L3-p53PmlEagPuroΔTK-1L plasmid (see above) by HindIII and FseI digestion, resulting in the p53GFP exchange construct, the sequence which was verified before use.
T5930 863-865 PRP denotes We
T5931 866-874 VBD denotes verified
T5932 875-878 DT denotes the
T5933 879-887 NN denotes sequence
T5934 888-890 IN denotes of
T5935 891-895 DT denotes this
T5937 896-899 NN denotes p53
T5939 899-900 HYPH denotes -
T5938 900-903 NN denotes GFP
T5940 904-910 NN denotes fusion
T5936 911-919 NN denotes fragment
T5941 919-921 , denotes ,
T5942 921-925 RB denotes then
T5943 926-933 VBD denotes swapped
T5944 934-936 PRP denotes it
T5945 937-939 IN denotes in
T5946 940-943 DT denotes the
T5948 944-946 NN denotes L3
T5950 946-947 HYPH denotes -
T5951 947-963 NN denotes p53PmlEagPuroΔTK
T5952 963-964 HYPH denotes -
T5949 964-966 NN denotes 1L
T5947 967-974 NN denotes plasmid
T5953 975-976 -LRB- denotes (
T5954 976-979 VB denotes see
T5955 980-985 RB denotes above
T5956 985-986 -RRB- denotes )
T5957 987-989 IN denotes by
T5958 990-997 NN denotes HindIII
T5960 998-1001 CC denotes and
T5961 1002-1006 NN denotes FseI
T5959 1007-1016 NN denotes digestion
T5962 1016-1018 , denotes ,
T5963 1018-1027 VBG denotes resulting
T5964 1028-1030 IN denotes in
T5965 1031-1034 DT denotes the
T5967 1035-1041 NN denotes p53GFP
T5968 1042-1050 NN denotes exchange
T5966 1051-1060 NN denotes construct
T5969 1060-1062 , denotes ,
T5970 1062-1065 DT denotes the
T5971 1066-1074 NN denotes sequence
T5972 1075-1080 WDT denotes which
T5974 1081-1084 VBD denotes was
T5973 1085-1093 VBN denotes verified
T5975 1094-1100 IN denotes before
T5976 1101-1104 NN denotes use
T5977 1104-1105 . denotes .
T5978 1105-1281 sentence denotes The p53ΔPGFP exchange construct was engineered by combining sequences from the p53GFP exchange plasmid and sequences from the p53ΔP targeting construct described recently (5).
T5979 1106-1109 DT denotes The
T5981 1110-1118 NN denotes p53ΔPGFP
T5982 1119-1127 NN denotes exchange
T5980 1128-1137 NN denotes construct
T5984 1138-1141 VBD denotes was
T5983 1142-1152 VBN denotes engineered
T5985 1153-1155 IN denotes by
T5986 1156-1165 VBG denotes combining
T5987 1166-1175 NNS denotes sequences
T5988 1176-1180 IN denotes from
T5989 1181-1184 DT denotes the
T5991 1185-1191 NN denotes p53GFP
T5992 1192-1200 NN denotes exchange
T5990 1201-1208 NN denotes plasmid
T5993 1209-1212 CC denotes and
T5994 1213-1222 NNS denotes sequences
T5995 1223-1227 IN denotes from
T5996 1228-1231 DT denotes the
T5998 1232-1237 NN denotes p53ΔP
T5999 1238-1247 NN denotes targeting
T5997 1248-1257 NN denotes construct
T6000 1258-1267 VBN denotes described
T6001 1268-1276 RB denotes recently
T6002 1277-1278 -LRB- denotes (
T6003 1278-1279 CD denotes 5
T6004 1279-1280 -RRB- denotes )
T6005 1280-1281 . denotes .
T6006 1281-1324 sentence denotes Its sequence was also verified before use.
T6007 1282-1285 PRP$ denotes Its
T6008 1286-1294 NN denotes sequence
T6010 1295-1298 VBD denotes was
T6011 1299-1303 RB denotes also
T6009 1304-1312 VBN denotes verified
T6012 1313-1319 IN denotes before
T6013 1320-1323 NN denotes use
T6014 1323-1324 . denotes .
R1618 T5752 T5753 compound Exchange,constructs
R1619 T5754 T5753 punct : ,constructs
R1620 T5755 T5753 acl making,constructs
R1621 T5756 T5757 det the,p53GFP
R1622 T5757 T5758 nmod p53GFP,plasmids
R1623 T5758 T5755 dobj plasmids,making
R1624 T5759 T5757 cc and,p53GFP
R1625 T5760 T5761 compound p53,PGFP
R1626 T5761 T5757 conj PGFP,p53GFP
R1627 T5762 T5761 compound Δ,PGFP
R1628 T5764 T5765 aux To,make
R1629 T5765 T5766 advcl make,subcloned
R1630 T5767 T5768 det a,protein
R1631 T5768 T5765 dobj protein,make
R1632 T5769 T5770 compound p53,GFP
R1633 T5770 T5768 compound GFP,protein
R1634 T5771 T5770 punct -,GFP
R1635 T5772 T5768 compound fusion,protein
R1636 T5773 T5766 punct ", ",subcloned
R1637 T5774 T5766 nsubj we,subcloned
R1638 T5775 T5766 advmod first,subcloned
R1639 T5776 T5777 det a,fragment
R1640 T5777 T5766 dobj fragment,subcloned
R1641 T5778 T5779 compound SacII,HindIII
R1642 T5779 T5777 compound HindIII,fragment
R1643 T5780 T5779 punct -,HindIII
R1644 T5781 T5777 prep of,fragment
R1645 T5782 T5783 det the,locus
R1646 T5783 T5781 pobj locus,of
R1647 T5784 T5783 compound p53,locus
R1648 T5785 T5777 punct (,fragment
R1649 T5786 T5777 acl corresponding,fragment
R1650 T5787 T5786 prep to,corresponding
R1651 T5788 T5787 pobj part,to
R1652 T5789 T5788 prep of,part
R1653 T5790 T5789 pobj exon,of
R1654 T5791 T5790 nummod 10,exon
R1655 T5792 T5786 prep to,corresponding
R1656 T5793 T5792 pobj sequences,to
R1657 T5794 T5793 advmod downstream,sequences
R1658 T5795 T5794 prep of,downstream
R1659 T5796 T5797 det the,gene
R1660 T5797 T5795 pobj gene,of
R1661 T5798 T5766 punct ),subcloned
R1662 T5799 T5766 prep into,subcloned
R1663 T5800 T5799 pobj pBS,into
R1664 T5801 T5766 punct ", ",subcloned
R1665 T5802 T5803 advmod then,mutated
R1666 T5803 T5766 dep mutated,subcloned
R1667 T5804 T5805 det the,site
R1668 T5805 T5803 dobj site,mutated
R1669 T5806 T5805 compound HindIII,site
R1670 T5807 T5803 prep into,mutated
R1671 T5808 T5809 det a,site
R1672 T5809 T5807 pobj site,into
R1673 T5810 T5809 compound FseI,site
R1674 T5811 T5766 punct .,subcloned
R1675 T5813 T5814 nsubj We,mutated
R1676 T5815 T5814 advmod next,mutated
R1677 T5816 T5817 det the,part
R1678 T5817 T5814 dobj part,mutated
R1679 T5818 T5819 npadvmod C,terminal
R1680 T5819 T5817 amod terminal,part
R1681 T5820 T5819 punct -,terminal
R1682 T5821 T5817 prep of,part
R1683 T5822 T5823 det the,gene
R1684 T5823 T5821 pobj gene,of
R1685 T5824 T5823 compound p53,gene
R1686 T5825 T5814 prep in,mutated
R1687 T5826 T5827 nummod two,rounds
R1688 T5827 T5825 pobj rounds,in
R1689 T5828 T5827 prep of,rounds
R1690 T5829 T5830 compound PCR,mutagenesis
R1691 T5830 T5828 pobj mutagenesis,of
R1692 T5831 T5814 punct ", ",mutated
R1693 T5832 T5833 advmod first,with
R1694 T5833 T5814 prep with,mutated
R1695 T5834 T5835 nmod primers,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1696 T5835 T5833 pobj GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC,with
R1697 T5836 T5835 nummod 5,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1698 T5837 T5836 punct ′,5
R1699 T5838 T5835 punct -,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1700 T5839 T5835 punct -,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1701 T5840 T5835 nummod 3,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1702 T5841 T5835 punct ′,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1703 T5842 T5835 cc and,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1704 T5843 T5844 nummod 5,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1705 T5844 T5835 conj GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1706 T5845 T5843 punct ′,5
R1707 T5846 T5844 punct -,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1708 T5847 T5844 punct -,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1709 T5848 T5844 nummod 3,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1710 T5849 T5844 punct ′,GACGGGATGCAGAGGGGATCCGTCTGAGTCAGGCCC
R1711 T5850 T5835 punct ", ",GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1712 T5851 T5852 dep which,removed
R1713 T5852 T5835 relcl removed,GGGCCTGACTCAGACGGATCCCCTCTGCATCCCGTC
R1714 T5853 T5854 det the,codon
R1715 T5854 T5852 dobj codon,removed
R1716 T5855 T5854 compound stop,codon
R1717 T5856 T5852 cc and,removed
R1718 T5857 T5852 conj introduced,removed
R1719 T5858 T5859 det a,site
R1720 T5859 T5857 dobj site,introduced
R1721 T5860 T5859 compound BamHI,site
R1722 T5861 T5833 punct ", ",with
R1723 T5862 T5863 advmod then,with
R1724 T5863 T5833 prep with,with
R1725 T5864 T5865 nmod primers,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1726 T5865 T5863 pobj GACGGATCCCCTCTGAATTCCGTCCCCATCACCA,with
R1727 T5866 T5865 nummod 5,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1728 T5867 T5866 punct ′,5
R1729 T5868 T5865 punct -,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1730 T5869 T5865 punct -,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1731 T5870 T5865 nummod 3,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1732 T5871 T5865 punct ′,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1733 T5872 T5865 cc and,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1734 T5873 T5874 nummod 5,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1735 T5874 T5865 conj TGGTGATGGGGACGGAATACAGAGGGGATCCGTC,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1736 T5875 T5873 punct ′,5
R1737 T5876 T5874 punct -,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1738 T5877 T5874 punct -,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1739 T5878 T5874 nummod 3,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1740 T5879 T5874 punct ′,TGGTGATGGGGACGGAATACAGAGGGGATCCGTC
R1741 T5880 T5865 punct ", ",GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1742 T5881 T5882 dep which,introduced
R1743 T5882 T5865 relcl introduced,GACGGATCCCCTCTGAATTCCGTCCCCATCACCA
R1744 T5883 T5884 det an,site
R1745 T5884 T5882 dobj site,introduced
R1746 T5885 T5884 compound EcoRI,site
R1747 T5886 T5814 punct .,mutated
R1748 T5888 T5889 nsubj We,verified
R1749 T5890 T5891 det the,sequence
R1750 T5891 T5889 dobj sequence,verified
R1751 T5892 T5891 prep from,sequence
R1752 T5893 T5894 det the,plasmid
R1753 T5894 T5892 pobj plasmid,from
R1754 T5895 T5894 amod mutated,plasmid
R1755 T5896 T5889 punct ", ",verified
R1756 T5897 T5898 advmod then,digested
R1757 T5898 T5889 dep digested,verified
R1758 T5899 T5898 dobj it,digested
R1759 T5900 T5898 prep with,digested
R1760 T5901 T5900 pobj BamHI,with
R1761 T5902 T5901 cc and,BamHI
R1762 T5903 T5901 conj EcoRI,BamHI
R1763 T5904 T5889 punct ", ",verified
R1764 T5905 T5906 aux to,insert
R1765 T5906 T5889 advcl insert,verified
R1766 T5907 T5906 prep in,insert
R1767 T5908 T5909 compound frame,GFP
R1768 T5909 T5910 compound GFP,sequences
R1769 T5910 T5907 pobj sequences,in
R1770 T5911 T5910 prep from,sequences
R1771 T5912 T5913 det a,fragment
R1772 T5913 T5911 pobj fragment,from
R1773 T5914 T5915 compound Bam,EcoRI
R1774 T5915 T5913 compound EcoRI,fragment
R1775 T5916 T5915 compound HI,EcoRI
R1776 T5917 T5915 punct -,EcoRI
R1777 T5918 T5913 prep of,fragment
R1778 T5919 T5920 compound plasmid,GFP
R1779 T5920 T5918 pobj GFP,of
R1780 T5921 T5920 compound phr,GFP
R1781 T5922 T5920 punct -,GFP
R1782 T5923 T5920 punct -,GFP
R1783 T5924 T5920 nummod 1,GFP
R1784 T5925 T5926 punct (,Stratagene
R1785 T5926 T5913 parataxis Stratagene,fragment
R1786 T5927 T5926 punct ),Stratagene
R1787 T5928 T5889 punct .,verified
R1788 T5930 T5931 nsubj We,verified
R1789 T5932 T5933 det the,sequence
R1790 T5933 T5931 dobj sequence,verified
R1791 T5934 T5933 prep of,sequence
R1792 T5935 T5936 det this,fragment
R1793 T5936 T5934 pobj fragment,of
R1794 T5937 T5938 compound p53,GFP
R1795 T5938 T5940 compound GFP,fusion
R1796 T5939 T5938 punct -,GFP
R1797 T5940 T5936 compound fusion,fragment
R1798 T5941 T5931 punct ", ",verified
R1799 T5942 T5943 advmod then,swapped
R1800 T5943 T5931 dep swapped,verified
R1801 T5944 T5943 dobj it,swapped
R1802 T5945 T5943 prep in,swapped
R1803 T5946 T5947 det the,plasmid
R1804 T5947 T5945 pobj plasmid,in
R1805 T5948 T5949 compound L3,1L
R1806 T5949 T5947 compound 1L,plasmid
R1807 T5950 T5949 punct -,1L
R1808 T5951 T5949 compound p53PmlEagPuroΔTK,1L
R1809 T5952 T5949 punct -,1L
R1810 T5953 T5954 punct (,see
R1811 T5954 T5943 parataxis see,swapped
R1812 T5955 T5954 advmod above,see
R1813 T5956 T5954 punct ),see
R1814 T5957 T5943 prep by,swapped
R1815 T5958 T5959 nmod HindIII,digestion
R1816 T5959 T5957 pobj digestion,by
R1817 T5960 T5958 cc and,HindIII
R1818 T5961 T5958 conj FseI,HindIII
R1819 T5962 T5943 punct ", ",swapped
R1820 T5963 T5943 advcl resulting,swapped
R1821 T5964 T5963 prep in,resulting
R1822 T5965 T5966 det the,construct
R1823 T5966 T5964 pobj construct,in
R1824 T5967 T5968 compound p53GFP,exchange
R1825 T5968 T5966 compound exchange,construct
R1826 T5969 T5966 punct ", ",construct
R1827 T5970 T5971 det the,sequence
R1828 T5971 T5966 appos sequence,construct
R1829 T5972 T5973 dep which,verified
R1830 T5973 T5971 relcl verified,sequence
R1831 T5974 T5973 auxpass was,verified
R1832 T5975 T5973 prep before,verified
R1833 T5976 T5975 pobj use,before
R1834 T5977 T5931 punct .,verified
R1835 T5979 T5980 det The,construct
R1836 T5980 T5983 nsubjpass construct,engineered
R1837 T5981 T5982 compound p53ΔPGFP,exchange
R1838 T5982 T5980 compound exchange,construct
R1839 T5984 T5983 auxpass was,engineered
R1840 T5985 T5983 prep by,engineered
R1841 T5986 T5985 pcomp combining,by
R1842 T5987 T5986 dobj sequences,combining
R1843 T5988 T5987 prep from,sequences
R1844 T5989 T5990 det the,plasmid
R1845 T5990 T5988 pobj plasmid,from
R1846 T5991 T5990 compound p53GFP,plasmid
R1847 T5992 T5990 compound exchange,plasmid
R1848 T5993 T5987 cc and,sequences
R1849 T5994 T5987 conj sequences,sequences
R1850 T5995 T5994 prep from,sequences
R1851 T5996 T5997 det the,construct
R1852 T5997 T5995 pobj construct,from
R1853 T5998 T5997 compound p53ΔP,construct
R1854 T5999 T5997 compound targeting,construct
R1855 T6000 T5987 acl described,sequences
R1856 T6001 T6000 advmod recently,described
R1857 T6002 T6003 punct (,5
R1858 T6003 T5986 parataxis 5,combining
R1859 T6004 T6003 punct ),5
R1860 T6005 T5983 punct .,engineered
R1861 T6007 T6008 poss Its,sequence
R1862 T6008 T6009 nsubjpass sequence,verified
R1863 T6010 T6009 auxpass was,verified
R1864 T6011 T6009 advmod also,verified
R1865 T6012 T6009 prep before,verified
R1866 T6013 T6012 pobj use,before
R1867 T6014 T6009 punct .,verified