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PMC:1540739 / 3416-5012 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T2973 0-4 RB denotes Here
T2975 5-7 PRP denotes we
T2974 8-14 VBP denotes report
T2976 15-17 DT denotes an
T2977 18-26 NN denotes approach
T2978 27-31 WDT denotes that
T2979 32-39 VBZ denotes enables
T2980 40-46 RB denotes highly
T2981 47-56 JJ denotes efficient
T2982 57-66 NN denotes targeting
T2983 67-69 IN denotes at
T2984 70-73 DT denotes the
T2986 74-77 NN denotes p53
T2985 78-83 NN denotes locus
T2987 84-86 IN denotes in
T2988 87-91 CC denotes both
T2990 92-94 NN denotes ES
T2989 95-100 NNS denotes cells
T2991 101-104 CC denotes and
T2992 105-116 NNS denotes fibroblasts
T2993 116-117 . denotes .
T2994 117-413 sentence denotes Recombinase-Mediated Cassette Exchange (RMCE) approaches were developed to improve targeting efficiency using a two-step process: the gene of interest is first replaced by a selection cassette flanked by recombinase target sites (e.g. loxP sites for Cre recombinase, to create a ‘floxed’ locus).
T2995 118-129 NN denotes Recombinase
T2997 129-130 HYPH denotes -
T2996 130-138 JJ denotes Mediated
T2999 139-147 NNP denotes Cassette
T2998 148-156 NNP denotes Exchange
T3001 157-158 -LRB- denotes (
T3002 158-162 NN denotes RMCE
T3003 162-163 -RRB- denotes )
T3000 164-174 NNS denotes approaches
T3005 175-179 VBD denotes were
T3004 180-189 VBN denotes developed
T3007 190-192 TO denotes to
T3008 193-200 VB denotes improve
T3009 201-210 VBG denotes targeting
T3010 211-221 NN denotes efficiency
T3011 222-227 VBG denotes using
T3012 228-229 DT denotes a
T3014 230-233 CD denotes two
T3016 233-234 HYPH denotes -
T3015 234-238 NN denotes step
T3013 239-246 NN denotes process
T3017 246-248 : denotes :
T3018 248-251 DT denotes the
T3019 252-256 NN denotes gene
T3020 257-259 IN denotes of
T3021 260-268 NN denotes interest
T3022 269-271 VBZ denotes is
T3023 272-277 RB denotes first
T3006 278-286 VBN denotes replaced
T3024 287-289 IN denotes by
T3025 290-291 DT denotes a
T3027 292-301 NN denotes selection
T3026 302-310 NN denotes cassette
T3028 311-318 VBN denotes flanked
T3029 319-321 IN denotes by
T3030 322-333 NN denotes recombinase
T3032 334-340 NN denotes target
T3031 341-346 NNS denotes sites
T3033 347-348 -LRB- denotes (
T3035 348-352 FW denotes e.g.
T3036 353-357 NN denotes loxP
T3034 358-363 NNS denotes sites
T3037 364-367 IN denotes for
T3038 368-371 NN denotes Cre
T3039 372-383 NN denotes recombinase
T3040 383-385 , denotes ,
T3041 385-387 TO denotes to
T3042 388-394 VB denotes create
T3043 395-396 DT denotes a
T3045 397-398 `` denotes ‘
T3046 398-404 JJ denotes floxed
T3047 404-405 '' denotes ’
T3044 406-411 NN denotes locus
T3048 411-412 -RRB- denotes )
T3049 412-413 . denotes .
T3050 413-573 sentence denotes Then, Cre-mediated recombination in the presence of a cassette containing a floxed mutant allele removes the resident sequence and inserts the mutant gene (7).
T3051 414-418 RB denotes Then
T3053 418-420 , denotes ,
T3054 420-423 NN denotes Cre
T3056 423-424 HYPH denotes -
T3055 424-432 JJ denotes mediated
T3057 433-446 NN denotes recombination
T3058 447-449 IN denotes in
T3059 450-453 DT denotes the
T3060 454-462 NN denotes presence
T3061 463-465 IN denotes of
T3062 466-467 DT denotes a
T3063 468-476 NN denotes cassette
T3064 477-487 VBG denotes containing
T3065 488-489 DT denotes a
T3067 490-496 VBN denotes floxed
T3068 497-503 JJ denotes mutant
T3066 504-510 NN denotes allele
T3052 511-518 VBZ denotes removes
T3069 519-522 DT denotes the
T3071 523-531 JJ denotes resident
T3070 532-540 NN denotes sequence
T3072 541-544 CC denotes and
T3073 545-552 VBZ denotes inserts
T3074 553-556 DT denotes the
T3076 557-563 JJ denotes mutant
T3075 564-568 NN denotes gene
T3077 569-570 -LRB- denotes (
T3078 570-571 CD denotes 7
T3079 571-572 -RRB- denotes )
T3080 572-573 . denotes .
T3081 573-682 sentence denotes Previously, technical difficulties have prevented RMCE from being applied routinely to generate mutant mice.
T3082 574-584 RB denotes Previously
T3084 584-586 , denotes ,
T3085 586-595 JJ denotes technical
T3086 596-608 NNS denotes difficulties
T3087 609-613 VBD denotes have
T3083 614-623 VBN denotes prevented
T3088 624-628 NN denotes RMCE
T3089 629-633 IN denotes from
T3090 634-639 VBG denotes being
T3091 640-647 VBN denotes applied
T3092 648-657 RB denotes routinely
T3093 658-660 TO denotes to
T3094 661-669 VB denotes generate
T3095 670-676 JJ denotes mutant
T3096 677-681 NNS denotes mice
T3097 681-682 . denotes .
T3098 682-828 sentence denotes For example, exchanges using cassettes with directly repeated loxP sites were inefficient because excisions dominated the intended exchanges (8).
T3099 683-686 IN denotes For
T3101 687-694 NN denotes example
T3102 694-696 , denotes ,
T3103 696-705 NNS denotes exchanges
T3104 706-711 VBG denotes using
T3105 712-721 NNS denotes cassettes
T3106 722-726 IN denotes with
T3107 727-735 RB denotes directly
T3108 736-744 VBN denotes repeated
T3110 745-749 NN denotes loxP
T3109 750-755 NNS denotes sites
T3100 756-760 VBD denotes were
T3111 761-772 JJ denotes inefficient
T3112 773-780 IN denotes because
T3114 781-790 NNS denotes excisions
T3113 791-800 VBD denotes dominated
T3115 801-804 DT denotes the
T3117 805-813 VBN denotes intended
T3116 814-823 NNS denotes exchanges
T3118 824-825 -LRB- denotes (
T3119 825-826 CD denotes 8
T3120 826-827 -RRB- denotes )
T3121 827-828 . denotes .
T3122 828-1096 sentence denotes loxP sites with different sequences were generated to overcome this problem, but these sites also underwent intramolecular recombination, making RMCE efficient only if the replacement cassette contained a marker enabling selection of the desired recombinant (7,9–12).
T3123 829-833 NN denotes loxP
T3124 834-839 NNS denotes sites
T3126 840-844 IN denotes with
T3127 845-854 JJ denotes different
T3128 855-864 NNS denotes sequences
T3129 865-869 VBD denotes were
T3125 870-879 VBN denotes generated
T3130 880-882 TO denotes to
T3131 883-891 VB denotes overcome
T3132 892-896 DT denotes this
T3133 897-904 NN denotes problem
T3134 904-906 , denotes ,
T3135 906-909 CC denotes but
T3136 910-915 DT denotes these
T3137 916-921 NNS denotes sites
T3139 922-926 RB denotes also
T3138 927-936 VBD denotes underwent
T3140 937-951 JJ denotes intramolecular
T3141 952-965 NN denotes recombination
T3142 965-967 , denotes ,
T3143 967-973 VBG denotes making
T3144 974-978 NN denotes RMCE
T3145 979-988 JJ denotes efficient
T3146 989-993 RB denotes only
T3148 994-996 IN denotes if
T3149 997-1000 DT denotes the
T3151 1001-1012 NN denotes replacement
T3150 1013-1021 NN denotes cassette
T3147 1022-1031 VBD denotes contained
T3152 1032-1033 DT denotes a
T3153 1034-1040 NN denotes marker
T3154 1041-1049 VBG denotes enabling
T3155 1050-1059 NN denotes selection
T3156 1060-1062 IN denotes of
T3157 1063-1066 DT denotes the
T3159 1067-1074 VBN denotes desired
T3158 1075-1086 JJ denotes recombinant
T3160 1087-1088 -LRB- denotes (
T3161 1088-1089 CD denotes 7
T3162 1089-1090 , denotes ,
T3163 1090-1091 CD denotes 9
T3164 1091-1092 SYM denotes –
T3165 1092-1094 CD denotes 12
T3166 1094-1095 -RRB- denotes )
T3167 1095-1096 . denotes .
T3168 1096-1252 sentence denotes However, interference resulting from expression of the selection marker and the endogenous gene (13) necessitates strategies to remove the selectable gene.
T3169 1097-1104 RB denotes However
T3171 1104-1106 , denotes ,
T3172 1106-1118 NN denotes interference
T3173 1119-1128 VBG denotes resulting
T3174 1129-1133 IN denotes from
T3175 1134-1144 NN denotes expression
T3176 1145-1147 IN denotes of
T3177 1148-1151 DT denotes the
T3179 1152-1161 NN denotes selection
T3178 1162-1168 NN denotes marker
T3180 1169-1172 CC denotes and
T3181 1173-1176 DT denotes the
T3183 1177-1187 JJ denotes endogenous
T3182 1188-1192 NN denotes gene
T3184 1193-1194 -LRB- denotes (
T3185 1194-1196 CD denotes 13
T3186 1196-1197 -RRB- denotes )
T3170 1198-1210 VBZ denotes necessitates
T3187 1211-1221 NNS denotes strategies
T3188 1222-1224 TO denotes to
T3189 1225-1231 VB denotes remove
T3190 1232-1235 DT denotes the
T3192 1236-1246 JJ denotes selectable
T3191 1247-1251 NN denotes gene
T3193 1251-1252 . denotes .
T3194 1252-1596 sentence denotes Together, previous studies indicate that an optimal RMCE requires (i) inverted heterologous loxP sites diverging by at least 2 nt to maximize the efficiency of exchange and (ii) an expression cassette enabling both positive selection to identify the initial recombinant and then negative selection to obtain a ‘marker-free’ mutant allele (14).
T3195 1253-1261 RB denotes Together
T3197 1261-1263 , denotes ,
T3198 1263-1271 JJ denotes previous
T3199 1272-1279 NNS denotes studies
T3196 1280-1288 VBP denotes indicate
T3200 1289-1293 IN denotes that
T3202 1294-1296 DT denotes an
T3204 1297-1304 JJ denotes optimal
T3203 1305-1309 NN denotes RMCE
T3201 1310-1318 VBZ denotes requires
T3205 1319-1320 -LRB- denotes (
T3206 1320-1321 LS denotes i
T3208 1321-1322 -RRB- denotes )
T3209 1323-1331 VBN denotes inverted
T3210 1332-1344 JJ denotes heterologous
T3211 1345-1349 NN denotes loxP
T3207 1350-1355 NNS denotes sites
T3212 1356-1365 VBG denotes diverging
T3213 1366-1368 IN denotes by
T3214 1369-1371 RB denotes at
T3216 1372-1377 RBS denotes least
T3215 1378-1379 CD denotes 2
T3217 1380-1382 NN denotes nt
T3218 1383-1385 TO denotes to
T3219 1386-1394 VB denotes maximize
T3220 1395-1398 DT denotes the
T3221 1399-1409 NN denotes efficiency
T3222 1410-1412 IN denotes of
T3223 1413-1421 NN denotes exchange
T3224 1422-1425 CC denotes and
T3225 1426-1427 -LRB- denotes (
T3226 1427-1429 LS denotes ii
T3228 1429-1430 -RRB- denotes )
T3229 1431-1433 DT denotes an
T3230 1434-1444 NN denotes expression
T3227 1445-1453 NN denotes cassette
T3231 1454-1462 VBG denotes enabling
T3232 1463-1467 CC denotes both
T3234 1468-1476 JJ denotes positive
T3233 1477-1486 NN denotes selection
T3235 1487-1489 TO denotes to
T3236 1490-1498 VB denotes identify
T3237 1499-1502 DT denotes the
T3239 1503-1510 JJ denotes initial
T3238 1511-1522 JJ denotes recombinant
T3240 1523-1526 CC denotes and
T3241 1527-1531 RB denotes then
T3243 1532-1540 JJ denotes negative
T3242 1541-1550 NN denotes selection
T3244 1551-1553 TO denotes to
T3245 1554-1560 VB denotes obtain
T3246 1561-1562 DT denotes a
T3248 1563-1564 `` denotes ‘
T3250 1564-1570 NN denotes marker
T3251 1570-1571 HYPH denotes -
T3249 1571-1575 JJ denotes free
T3252 1575-1576 '' denotes ’
T3253 1577-1583 JJ denotes mutant
T3247 1584-1590 NN denotes allele
T3254 1591-1592 -LRB- denotes (
T3255 1592-1594 CD denotes 14
T3256 1594-1595 -RRB- denotes )
T3257 1595-1596 . denotes .
R543 T3013 T3011 dobj process,using
R545 T3014 T3015 nummod two,step
R546 T3015 T3013 compound step,process
R547 T3016 T3015 punct -,step
R549 T3017 T3006 punct : ,replaced
R550 T3018 T3019 det the,gene
R552 T3019 T3006 nsubjpass gene,replaced
R553 T3020 T3019 prep of,gene
R554 T3021 T3020 pobj interest,of
R556 T3022 T3006 auxpass is,replaced
R557 T3023 T3006 advmod first,replaced
R558 T3024 T3006 agent by,replaced
R560 T3025 T3026 det a,cassette
R561 T3026 T3024 pobj cassette,by
R562 T3027 T3026 compound selection,cassette
R563 T3028 T3026 acl flanked,cassette
R564 T3029 T3028 agent by,flanked
R565 T3030 T3031 compound recombinase,sites
R567 T3031 T3029 pobj sites,by
R568 T3032 T3031 compound target,sites
R569 T3033 T3034 punct (,sites
R571 T3034 T3031 parataxis sites,sites
R572 T3035 T3034 advmod e.g.,sites
R573 T3036 T3034 compound loxP,sites
R575 T3037 T3034 prep for,sites
R576 T3038 T3039 compound Cre,recombinase
R577 T3039 T3037 pobj recombinase,for
R579 T3040 T3034 punct ", ",sites
R581 T3041 T3042 aux to,create
R582 T3042 T3034 advcl create,sites
R584 T3043 T3044 det a,locus
R585 T3044 T3042 dobj locus,create
R586 T3045 T3044 punct ‘,locus
R588 T3046 T3044 amod floxed,locus
R589 T3047 T3044 punct ’,locus
R590 T3048 T3034 punct ),sites
R591 T3049 T3006 punct .,replaced
R593 T3051 T3052 advmod Then,removes
R594 T3053 T3052 punct ", ",removes
R597 T3054 T3055 npadvmod Cre,mediated
R598 T3055 T3057 amod mediated,recombination
R599 T3056 T3055 punct -,mediated
R601 T3057 T3052 nsubj recombination,removes
R602 T3058 T3057 prep in,recombination
R603 T3059 T3060 det the,presence
R605 T3060 T3058 pobj presence,in
R606 T3061 T3060 prep of,presence
R607 T3062 T3063 det a,cassette
R609 T3063 T3061 pobj cassette,of
R610 T3064 T3063 acl containing,cassette
R612 T3065 T3066 det a,allele
R613 T3066 T3064 dobj allele,containing
R614 T3067 T3066 amod floxed,allele
R615 T3068 T3066 amod mutant,allele
R616 T3069 T3070 det the,sequence
R617 T3070 T3052 dobj sequence,removes
R619 T3071 T3070 amod resident,sequence
R620 T3072 T3052 cc and,removes
R621 T3073 T3052 conj inserts,removes
R622 T3074 T3075 det the,gene
R624 T3075 T3073 dobj gene,inserts
R625 T3076 T3075 amod mutant,gene
R626 T3077 T3078 punct (,7
R628 T3078 T3073 parataxis 7,inserts
R629 T3079 T3078 punct ),7
R630 T3080 T3052 punct .,removes
R632 T3082 T3083 advmod Previously,prevented
R635 T3084 T3083 punct ", ",prevented
R636 T3085 T3086 amod technical,difficulties
R637 T3086 T3083 nsubj difficulties,prevented
R638 T3087 T3083 aux have,prevented
R640 T3088 T3083 dobj RMCE,prevented
R641 T3089 T3083 prep from,prevented
R642 T3090 T3091 auxpass being,applied
R644 T3091 T3089 pcomp applied,from
R645 T3092 T3091 advmod routinely,applied
R646 T3093 T3094 aux to,generate
R648 T3094 T3091 advcl generate,applied
R649 T3095 T3096 amod mutant,mice
R650 T3096 T3094 dobj mice,generate
R652 T3097 T3083 punct .,prevented
R654 T3099 T3100 prep For,were
R655 T3101 T3099 pobj example,For
R657 T3102 T3100 punct ", ",were
R658 T3103 T3100 nsubj exchanges,were
R659 T3104 T3103 acl using,exchanges
R661 T3105 T3104 dobj cassettes,using
R662 T3106 T3104 prep with,using
R663 T3107 T3108 advmod directly,repeated
R665 T3108 T3109 amod repeated,sites
R666 T3109 T3106 pobj sites,with
R667 T3110 T3109 compound loxP,sites
R668 T3111 T3100 acomp inefficient,were
R669 T3112 T3113 mark because,dominated
R670 T3113 T3100 advcl dominated,were
R672 T3114 T3113 nsubj excisions,dominated
R673 T3115 T3116 det the,exchanges
R674 T3116 T3113 dobj exchanges,dominated
R676 T3117 T3116 amod intended,exchanges
R677 T3118 T3119 punct (,8
R680 T3119 T3113 parataxis 8,dominated
R681 T2973 T2974 advmod Here,report
R682 T3120 T3119 punct ),8
R683 T3121 T3100 punct .,were
R684 T3123 T3124 compound loxP,sites
R685 T2975 T2974 nsubj we,report
R686 T3124 T3125 nsubjpass sites,generated
R687 T3126 T3124 prep with,sites
R688 T2976 T2977 det an,approach
R689 T3127 T3128 amod different,sequences
R690 T3128 T3126 pobj sequences,with
R691 T2977 T2974 dobj approach,report
R692 T3129 T3125 auxpass were,generated
R693 T3130 T3131 aux to,overcome
R694 T2978 T2979 dep that,enables
R695 T3131 T3125 advcl overcome,generated
R696 T3132 T3133 det this,problem
R697 T3133 T3131 dobj problem,overcome
R698 T2979 T2977 relcl enables,approach
R699 T3134 T3125 punct ", ",generated
R700 T3135 T3125 cc but,generated
R701 T3136 T3137 det these,sites
R702 T2980 T2981 advmod highly,efficient
R703 T3137 T3138 nsubj sites,underwent
R704 T3138 T3125 conj underwent,generated
R705 T3139 T3138 advmod also,underwent
R706 T2981 T2982 amod efficient,targeting
R707 T2982 T2979 dobj targeting,enables
R708 T3140 T3141 amod intramolecular,recombination
R709 T3141 T3138 dobj recombination,underwent
R710 T2983 T2979 prep at,enables
R711 T3142 T3138 punct ", ",underwent
R712 T3143 T3138 advcl making,underwent
R713 T2984 T2985 det the,locus
R714 T3144 T3145 nsubj RMCE,efficient
R715 T3145 T3143 ccomp efficient,making
R716 T3146 T3147 advmod only,contained
R717 T3147 T3143 advcl contained,making
R718 T3148 T3147 mark if,contained
R719 T2985 T2983 pobj locus,at
R720 T3149 T3150 det the,cassette
R721 T3150 T3147 nsubj cassette,contained
R722 T3151 T3150 compound replacement,cassette
R723 T3152 T3153 det a,marker
R724 T2986 T2985 compound p53,locus
R725 T3153 T3147 dobj marker,contained
R726 T3154 T3153 acl enabling,marker
R727 T2987 T2979 prep in,enables
R728 T3155 T3154 dobj selection,enabling
R729 T3156 T3155 prep of,selection
R730 T3157 T3158 det the,recombinant
R731 T3158 T3156 pobj recombinant,of
R732 T2988 T2989 preconj both,cells
R733 T3159 T3158 amod desired,recombinant
R734 T3160 T3161 punct (,7
R735 T2989 T2987 pobj cells,in
R736 T3161 T3147 parataxis 7,contained
R737 T3162 T3161 punct ",",7
R738 T3163 T3161 appos 9,7
R739 T2990 T2989 compound ES,cells
R740 T3164 T3165 punct –,12
R741 T3165 T3163 prep 12,9
R742 T2991 T2989 cc and,cells
R743 T3166 T3161 punct ),7
R744 T3167 T3138 punct .,underwent
R745 T2992 T2989 conj fibroblasts,cells
R746 T3169 T3170 advmod However,necessitates
R747 T3171 T3170 punct ", ",necessitates
R748 T3172 T3170 nsubj interference,necessitates
R749 T2993 T2974 punct .,report
R750 T3173 T3172 acl resulting,interference
R751 T3174 T3173 prep from,resulting
R752 T2995 T2996 npadvmod Recombinase,Mediated
R753 T3175 T3174 pobj expression,from
R754 T3176 T3175 prep of,expression
R755 T3177 T3178 det the,marker
R756 T3178 T3176 pobj marker,of
R757 T2996 T2998 amod Mediated,Exchange
R758 T3179 T3178 compound selection,marker
R759 T3180 T3178 cc and,marker
R760 T3181 T3182 det the,gene
R761 T2997 T2996 punct -,Mediated
R762 T3182 T3178 conj gene,marker
R763 T3183 T3182 amod endogenous,gene
R764 T3184 T3185 punct (,13
R765 T2998 T3000 nmod Exchange,approaches
R766 T3185 T3173 parataxis 13,resulting
R767 T3186 T3185 punct ),13
R768 T3187 T3170 dobj strategies,necessitates
R769 T3188 T3189 aux to,remove
R770 T3189 T3187 advcl remove,strategies
R771 T3190 T3191 det the,gene
R772 T3191 T3189 dobj gene,remove
R773 T2999 T2998 nmod Cassette,Exchange
R774 T3192 T3191 amod selectable,gene
R775 T3193 T3170 punct .,necessitates
R776 T3000 T3004 nsubjpass approaches,developed
R777 T3195 T3196 advmod Together,indicate
R778 T3197 T3196 punct ", ",indicate
R779 T3001 T2998 punct (,Exchange
R780 T3198 T3199 amod previous,studies
R781 T3199 T3196 nsubj studies,indicate
R782 T3200 T3201 mark that,requires
R783 T3002 T2998 appos RMCE,Exchange
R784 T3201 T3196 ccomp requires,indicate
R785 T3202 T3203 det an,RMCE
R786 T3003 T3000 punct ),approaches
R787 T3203 T3201 nsubj RMCE,requires
R788 T3204 T3203 amod optimal,RMCE
R789 T3205 T3206 punct (,i
R790 T3004 T3006 ccomp developed,replaced
R791 T3206 T3207 meta i,sites
R792 T3207 T3201 dobj sites,requires
R793 T3208 T3206 punct ),i
R794 T3005 T3004 auxpass were,developed
R795 T3209 T3207 amod inverted,sites
R796 T3007 T3008 aux to,improve
R797 T3210 T3207 amod heterologous,sites
R798 T3211 T3207 compound loxP,sites
R799 T3212 T3207 acl diverging,sites
R800 T3213 T3212 prep by,diverging
R801 T3214 T3215 quantmod at,2
R802 T3215 T3217 nummod 2,nt
R803 T3008 T3004 advcl improve,developed
R804 T3216 T3214 advmod least,at
R805 T3217 T3213 pobj nt,by
R806 T3218 T3219 aux to,maximize
R807 T3009 T3010 amod targeting,efficiency
R808 T3219 T3207 advcl maximize,sites
R809 T3220 T3221 det the,efficiency
R810 T3221 T3219 dobj efficiency,maximize
R811 T3010 T3008 dobj efficiency,improve
R812 T3222 T3221 prep of,efficiency
R813 T3223 T3222 pobj exchange,of
R814 T3011 T3008 advcl using,improve
R815 T3012 T3013 det a,process
R816 T3224 T3207 cc and,sites
R819 T3225 T3226 punct (,ii
R820 T3226 T3227 meta ii,cassette
R822 T3227 T3207 conj cassette,sites
R823 T3228 T3226 punct ),ii
R824 T3229 T3227 det an,cassette
R825 T3230 T3227 compound expression,cassette
R826 T3231 T3227 acl enabling,cassette
R827 T3232 T3233 preconj both,selection
R829 T3233 T3231 dobj selection,enabling
R830 T3234 T3233 amod positive,selection
R832 T3235 T3236 aux to,identify
R833 T3236 T3231 advcl identify,enabling
R834 T3237 T3238 det the,recombinant
R836 T3238 T3236 dobj recombinant,identify
R837 T3239 T3238 amod initial,recombinant
R838 T3240 T3231 cc and,enabling
R840 T3241 T3242 advmod then,selection
R841 T3242 T3231 conj selection,enabling
R842 T3243 T3242 amod negative,selection
R843 T3244 T3245 aux to,obtain
R845 T3245 T3242 advcl obtain,selection
R846 T3246 T3247 det a,allele
R847 T3247 T3245 dobj allele,obtain
R849 T3248 T3249 punct ‘,free
R850 T3249 T3247 amod free,allele
R852 T3250 T3249 npadvmod marker,free
R853 T3251 T3249 punct -,free
R854 T3252 T3249 punct ’,free
R856 T3253 T3247 amod mutant,allele
R857 T3254 T3255 punct (,14
R858 T3255 T3242 parataxis 14,selection
R860 T3256 T3255 punct ),14
R861 T3257 T3196 punct .,indicate