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PMC:1540739 / 2670-3415 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2844 0-12 NNS denotes Improvements
T2846 13-15 IN denotes in
T2847 16-23 JJ denotes current
T2848 24-36 NNS denotes technologies
T2849 37-40 VBP denotes are
T2845 41-47 VBN denotes needed
T2850 48-50 TO denotes to
T2851 51-57 VB denotes enable
T2852 58-62 JJ denotes such
T2853 63-71 NNS denotes analyses
T2854 72-74 TO denotes to
T2856 75-77 VB denotes be
T2855 78-85 VBN denotes applied
T2857 86-88 IN denotes to
T2858 89-92 DT denotes the
T2859 93-96 NN denotes p53
T2860 97-99 CC denotes or
T2861 100-105 JJ denotes other
T2862 106-111 NNS denotes genes
T2863 111-112 . denotes .
T2864 112-206 sentence denotes Developing methods to increase targeting efficiency in ES cells is clearly an important goal.
T2865 113-123 VBG denotes Developing
T2867 124-131 NNS denotes methods
T2868 132-134 TO denotes to
T2869 135-143 VB denotes increase
T2870 144-153 VBG denotes targeting
T2871 154-164 NN denotes efficiency
T2872 165-167 IN denotes in
T2873 168-170 NN denotes ES
T2874 171-176 NNS denotes cells
T2866 177-179 VBZ denotes is
T2875 180-187 RB denotes clearly
T2876 188-190 DT denotes an
T2878 191-200 JJ denotes important
T2877 201-205 NN denotes goal
T2879 205-206 . denotes .
T2880 206-307 sentence denotes In addition, efficient methods for gene targeting in fibroblasts could expedite phenotypic analyses.
T2881 207-209 IN denotes In
T2883 210-218 NN denotes addition
T2884 218-220 , denotes ,
T2885 220-229 JJ denotes efficient
T2886 230-237 NNS denotes methods
T2887 238-241 IN denotes for
T2888 242-246 NN denotes gene
T2889 247-256 NN denotes targeting
T2890 257-259 IN denotes in
T2891 260-271 NNS denotes fibroblasts
T2892 272-277 MD denotes could
T2882 278-286 VB denotes expedite
T2893 287-297 JJ denotes phenotypic
T2894 298-306 NNS denotes analyses
T2895 306-307 . denotes .
T2896 307-417 sentence denotes Indeed, siRNAs in fibroblasts often provide a faster read-out than equivalent gene knock-outs in animals (6).
T2897 308-314 RB denotes Indeed
T2899 314-316 , denotes ,
T2900 316-322 NNS denotes siRNAs
T2901 323-325 IN denotes in
T2902 326-337 NNS denotes fibroblasts
T2903 338-343 RB denotes often
T2898 344-351 VBP denotes provide
T2904 352-353 DT denotes a
T2906 354-360 JJR denotes faster
T2907 361-365 NN denotes read
T2908 365-366 HYPH denotes -
T2905 366-369 NN denotes out
T2909 370-374 IN denotes than
T2910 375-385 JJ denotes equivalent
T2912 386-390 NN denotes gene
T2913 391-396 NN denotes knock
T2914 396-397 HYPH denotes -
T2911 397-401 NNS denotes outs
T2915 402-404 IN denotes in
T2916 405-412 NNS denotes animals
T2917 413-414 -LRB- denotes (
T2918 414-415 CD denotes 6
T2919 415-416 -RRB- denotes )
T2920 416-417 . denotes .
T2921 417-553 sentence denotes However, modeling most disease-associated mutations requires generating subtle mutations, not knock-outs or reduced expression alleles.
T2922 418-425 RB denotes However
T2924 425-427 , denotes ,
T2925 427-435 VBG denotes modeling
T2926 436-440 RBS denotes most
T2928 441-448 NN denotes disease
T2930 448-449 HYPH denotes -
T2929 449-459 JJ denotes associated
T2927 460-469 NNS denotes mutations
T2923 470-478 VBZ denotes requires
T2931 479-489 VBG denotes generating
T2932 490-496 JJ denotes subtle
T2933 497-506 NNS denotes mutations
T2934 506-508 , denotes ,
T2935 508-511 CC denotes not
T2936 512-517 NN denotes knock
T2938 517-518 HYPH denotes -
T2937 518-522 NNS denotes outs
T2939 523-525 CC denotes or
T2940 526-533 VBN denotes reduced
T2941 534-544 NN denotes expression
T2942 545-552 NNS denotes alleles
T2943 552-553 . denotes .
T2944 553-745 sentence denotes Targeting point mutations in fibroblasts by homologous recombination is extremely inefficient, and targeting both alleles is required to reveal the phenotype of recessive autosomal mutations.
T2945 554-563 VBG denotes Targeting
T2947 564-569 NN denotes point
T2948 570-579 NNS denotes mutations
T2949 580-582 IN denotes in
T2950 583-594 NNS denotes fibroblasts
T2951 595-597 IN denotes by
T2952 598-608 JJ denotes homologous
T2953 609-622 NN denotes recombination
T2946 623-625 VBZ denotes is
T2954 626-635 RB denotes extremely
T2955 636-647 JJ denotes inefficient
T2956 647-649 , denotes ,
T2957 649-652 CC denotes and
T2958 653-662 VBG denotes targeting
T2960 663-667 DT denotes both
T2961 668-675 NNS denotes alleles
T2962 676-678 VBZ denotes is
T2959 679-687 VBN denotes required
T2963 688-690 TO denotes to
T2964 691-697 VB denotes reveal
T2965 698-701 DT denotes the
T2966 702-711 NN denotes phenotype
T2967 712-714 IN denotes of
T2968 715-724 JJ denotes recessive
T2969 725-734 JJ denotes autosomal
T2970 735-744 NNS denotes mutations
T2971 744-745 . denotes .
R460 T2844 T2845 nsubjpass Improvements,needed
R462 T2846 T2844 prep in,Improvements
R464 T2847 T2848 amod current,technologies
R465 T2848 T2846 pobj technologies,in
R466 T2849 T2845 auxpass are,needed
R468 T2850 T2851 aux to,enable
R469 T2851 T2845 advcl enable,needed
R470 T2907 T2905 compound read,out
R471 T2852 T2853 amod such,analyses
R472 T2853 T2851 dobj analyses,enable
R473 T2854 T2855 aux to,applied
R474 T2855 T2851 xcomp applied,enable
R475 T2908 T2905 punct -,out
R476 T2856 T2855 auxpass be,applied
R477 T2857 T2855 dative to,applied
R478 T2858 T2859 det the,p53
R479 T2909 T2905 prep than,out
R480 T2859 T2857 pobj p53,to
R481 T2860 T2859 cc or,p53
R482 T2910 T2911 amod equivalent,outs
R483 T2861 T2862 amod other,genes
R484 T2862 T2859 conj genes,p53
R485 T2863 T2845 punct .,needed
R486 T2911 T2909 pobj outs,than
R487 T2865 T2866 csubj Developing,is
R488 T2912 T2911 compound gene,outs
R489 T2867 T2865 dobj methods,Developing
R490 T2868 T2869 aux to,increase
R491 T2913 T2911 compound knock,outs
R492 T2869 T2867 advcl increase,methods
R493 T2870 T2871 amod targeting,efficiency
R494 T2871 T2869 dobj efficiency,increase
R495 T2872 T2869 prep in,increase
R496 T2914 T2911 punct -,outs
R497 T2873 T2874 compound ES,cells
R498 T2874 T2872 pobj cells,in
R499 T2915 T2911 prep in,outs
R500 T2875 T2866 advmod clearly,is
R501 T2876 T2877 det an,goal
R502 T2877 T2866 attr goal,is
R503 T2916 T2915 pobj animals,in
R504 T2878 T2877 amod important,goal
R505 T2917 T2918 punct (,6
R506 T2879 T2866 punct .,is
R507 T2918 T2898 parataxis 6,provide
R508 T2881 T2882 prep In,expedite
R509 T2883 T2881 pobj addition,In
R510 T2884 T2882 punct ", ",expedite
R511 T2885 T2886 amod efficient,methods
R512 T2919 T2918 punct ),6
R513 T2886 T2882 nsubj methods,expedite
R514 T2887 T2886 prep for,methods
R515 T2888 T2889 compound gene,targeting
R516 T2920 T2898 punct .,provide
R517 T2889 T2887 pobj targeting,for
R518 T2890 T2889 prep in,targeting
R519 T2922 T2923 advmod However,requires
R520 T2891 T2890 pobj fibroblasts,in
R521 T2892 T2882 aux could,expedite
R522 T2924 T2923 punct ", ",requires
R523 T2893 T2894 amod phenotypic,analyses
R524 T2894 T2882 dobj analyses,expedite
R525 T2895 T2882 punct .,expedite
R526 T2897 T2898 advmod Indeed,provide
R527 T2925 T2923 csubj modeling,requires
R528 T2899 T2898 punct ", ",provide
R529 T2926 T2927 advmod most,mutations
R530 T2900 T2898 nsubj siRNAs,provide
R531 T2901 T2900 prep in,siRNAs
R532 T2902 T2901 pobj fibroblasts,in
R533 T2927 T2925 dobj mutations,modeling
R534 T2903 T2898 advmod often,provide
R535 T2904 T2905 det a,out
R536 T2905 T2898 dobj out,provide
R537 T2906 T2905 amod faster,out
R538 T2928 T2929 npadvmod disease,associated
R539 T2929 T2927 amod associated,mutations
R540 T2930 T2929 punct -,associated
R541 T2931 T2923 xcomp generating,requires
R542 T2932 T2933 amod subtle,mutations
R544 T2933 T2931 dobj mutations,generating
R548 T2934 T2933 punct ", ",mutations
R551 T2935 T2933 cc not,mutations
R555 T2936 T2937 compound knock,outs
R559 T2937 T2933 conj outs,mutations
R566 T2938 T2937 punct -,outs
R570 T2939 T2937 cc or,outs
R574 T2940 T2941 amod reduced,expression
R578 T2941 T2942 compound expression,alleles
R580 T2942 T2937 conj alleles,outs
R583 T2943 T2923 punct .,requires
R587 T2945 T2946 csubj Targeting,is
R592 T2947 T2948 compound point,mutations
R595 T2948 T2945 dobj mutations,Targeting
R596 T2949 T2945 prep in,Targeting
R600 T2950 T2949 pobj fibroblasts,in
R604 T2951 T2945 prep by,Targeting
R608 T2952 T2953 amod homologous,recombination
R611 T2953 T2951 pobj recombination,by
R618 T2954 T2955 advmod extremely,inefficient
R623 T2955 T2946 acomp inefficient,is
R627 T2956 T2946 punct ", ",is
R631 T2957 T2946 cc and,is
R633 T2958 T2959 csubjpass targeting,required
R634 T2959 T2946 conj required,is
R639 T2960 T2961 det both,alleles
R643 T2961 T2958 dobj alleles,targeting
R647 T2962 T2959 auxpass is,required
R651 T2963 T2964 aux to,reveal
R653 T2964 T2959 advcl reveal,required
R656 T2965 T2966 det the,phenotype
R660 T2966 T2964 dobj phenotype,reveal
R664 T2967 T2966 prep of,phenotype
R671 T2968 T2969 amod recessive,autosomal
R675 T2969 T2970 amod autosomal,mutations
R678 T2970 T2967 pobj mutations,of
R679 T2971 T2959 punct .,required