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PMC:1540739 / 20559-21382 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T12988 0-5 DT denotes These
T12989 6-10 NNS denotes data
T12990 11-17 VBP denotes report
T12991 18-21 DT denotes the
T12992 22-33 NN denotes development
T12993 34-37 CC denotes and
T12994 38-52 NN denotes implementation
T12995 53-55 IN denotes of
T12996 56-58 DT denotes an
T12998 59-67 VBN denotes improved
T12999 68-72 NN denotes RMCE
T12997 73-81 NN denotes approach
T13000 82-86 WDT denotes that
T13001 87-94 VBZ denotes enables
T13002 95-104 JJ denotes efficient
T13004 105-111 NN denotes allele
T13003 112-124 NN denotes modification
T13005 125-127 IN denotes in
T13006 128-130 NN denotes ES
T13007 131-136 NNS denotes cells
T13008 137-139 TO denotes to
T13009 140-148 VB denotes generate
T13010 149-153 NNS denotes mice
T13011 154-157 CC denotes and
T13012 158-160 IN denotes in
T13014 161-173 JJ denotes heterozygous
T13015 174-178 NNS denotes MEFs
T13016 179-181 TO denotes to
T13013 182-192 VB denotes accelerate
T13017 193-203 JJ denotes phenotypic
T13018 204-212 NNS denotes analyses
T13019 212-213 . denotes .
T13020 213-476 sentence denotes The success of RMCE-ASAP relied on the integrated use of inverted heterologous loxP sites, a positive/negative selection marker that preserves the germline capacity of ES cells, and, for somatic cells, the existence of a knock-out allele of the gene of interest.
T13021 214-217 DT denotes The
T13022 218-225 NN denotes success
T13024 226-228 IN denotes of
T13025 229-233 NN denotes RMCE
T13027 233-234 HYPH denotes -
T13026 234-238 NN denotes ASAP
T13023 239-245 VBN denotes relied
T13028 246-248 IN denotes on
T13029 249-252 DT denotes the
T13031 253-263 VBN denotes integrated
T13030 264-267 NN denotes use
T13032 268-270 IN denotes of
T13033 271-279 VBN denotes inverted
T13035 280-292 JJ denotes heterologous
T13036 293-297 NN denotes loxP
T13034 298-303 NNS denotes sites
T13037 303-305 , denotes ,
T13038 305-306 DT denotes a
T13040 307-315 JJ denotes positive
T13042 315-316 HYPH denotes /
T13041 316-324 JJ denotes negative
T13043 325-334 NN denotes selection
T13039 335-341 NN denotes marker
T13044 342-346 WDT denotes that
T13045 347-356 VBZ denotes preserves
T13046 357-360 DT denotes the
T13048 361-369 NN denotes germline
T13047 370-378 NN denotes capacity
T13049 379-381 IN denotes of
T13050 382-384 NN denotes ES
T13051 385-390 NNS denotes cells
T13052 390-392 , denotes ,
T13053 392-395 CC denotes and
T13054 395-397 , denotes ,
T13055 397-400 IN denotes for
T13057 401-408 JJ denotes somatic
T13058 409-414 NNS denotes cells
T13059 414-416 , denotes ,
T13060 416-419 DT denotes the
T13056 420-429 NN denotes existence
T13061 430-432 IN denotes of
T13062 433-434 DT denotes a
T13064 435-440 VB denotes knock
T13065 440-441 HYPH denotes -
T13066 441-444 RP denotes out
T13063 445-451 NN denotes allele
T13067 452-454 IN denotes of
T13068 455-458 DT denotes the
T13069 459-463 NN denotes gene
T13070 464-466 IN denotes of
T13071 467-475 NN denotes interest
T13072 475-476 . denotes .
T13073 476-663 sentence denotes These characteristics should make RMCE-ASAP a robust and general technology for analysis of mammalian genes under conditions that preserve normal control mechanisms in different tissues.
T13074 477-482 DT denotes These
T13075 483-498 NNS denotes characteristics
T13077 499-505 MD denotes should
T13076 506-510 VB denotes make
T13078 511-515 NN denotes RMCE
T13080 515-516 HYPH denotes -
T13079 516-520 NN denotes ASAP
T13082 521-522 DT denotes a
T13083 523-529 JJ denotes robust
T13084 530-533 CC denotes and
T13085 534-541 JJ denotes general
T13081 542-552 NN denotes technology
T13086 553-556 IN denotes for
T13087 557-565 NN denotes analysis
T13088 566-568 IN denotes of
T13089 569-578 JJ denotes mammalian
T13090 579-584 NNS denotes genes
T13091 585-590 IN denotes under
T13092 591-601 NNS denotes conditions
T13093 602-606 WDT denotes that
T13094 607-615 VBP denotes preserve
T13095 616-622 JJ denotes normal
T13097 623-630 NN denotes control
T13096 631-641 NNS denotes mechanisms
T13098 642-644 IN denotes in
T13099 645-654 JJ denotes different
T13100 655-662 NNS denotes tissues
T13101 662-663 . denotes .
T13102 663-823 sentence denotes In addition, RMCE-ASAP could be used to generate fibroblastic cell lines tailored for the repeated targeting of widely studied genes (p53, c-myc, NF-KB, etc.).
T13103 664-666 IN denotes In
T13105 667-675 NN denotes addition
T13106 675-677 , denotes ,
T13107 677-681 NN denotes RMCE
T13109 681-682 HYPH denotes -
T13108 682-686 NN denotes ASAP
T13110 687-692 MD denotes could
T13111 693-695 VB denotes be
T13104 696-700 VBN denotes used
T13112 701-703 TO denotes to
T13113 704-712 VB denotes generate
T13114 713-725 JJ denotes fibroblastic
T13116 726-730 NN denotes cell
T13115 731-736 NNS denotes lines
T13117 737-745 VBN denotes tailored
T13118 746-749 IN denotes for
T13119 750-753 DT denotes the
T13121 754-762 VBN denotes repeated
T13120 763-772 NN denotes targeting
T13122 773-775 IN denotes of
T13123 776-782 RB denotes widely
T13124 783-790 VBN denotes studied
T13125 791-796 NNS denotes genes
T13126 797-798 -LRB- denotes (
T13127 798-801 NN denotes p53
T13128 801-803 , denotes ,
T13129 803-804 NN denotes c
T13131 804-805 HYPH denotes -
T13130 805-808 NN denotes myc
T13132 808-810 , denotes ,
T13133 810-812 NN denotes NF
T13135 812-813 HYPH denotes -
T13134 813-815 NN denotes KB
T13136 815-817 , denotes ,
T13137 817-821 FW denotes etc.
T13138 821-822 -RRB- denotes )
T13139 822-823 . denotes .
R3850 T13012 T13013 prep in,accelerate
R3852 T13013 T13001 conj accelerate,enables
R3853 T13014 T13015 amod heterozygous,MEFs
R3855 T13015 T13012 pobj MEFs,in
R3856 T13016 T13013 aux to,accelerate
R3857 T13017 T13018 amod phenotypic,analyses
R3859 T13018 T13013 dobj analyses,accelerate
R3860 T13019 T12990 punct .,report
R3861 T13021 T13022 det The,success
R3862 T13022 T13023 nsubj success,relied
R3864 T13024 T13022 prep of,success
R3865 T13025 T13026 compound RMCE,ASAP
R3867 T13026 T13024 pobj ASAP,of
R3868 T13027 T13026 punct -,ASAP
R3869 T13028 T13023 prep on,relied
R3870 T13029 T13030 det the,use
R3871 T13030 T13028 pobj use,on
R3873 T13031 T13030 amod integrated,use
R3874 T13032 T13030 prep of,use
R3875 T13033 T13034 amod inverted,sites
R3877 T13034 T13032 pobj sites,of
R3878 T13035 T13036 amod heterologous,loxP
R3879 T13036 T13034 compound loxP,sites
R3880 T13037 T13034 punct ", ",sites
R3882 T13038 T13039 det a,marker
R3883 T13039 T13034 conj marker,sites
R3885 T13040 T13041 amod positive,negative
R3886 T13041 T13043 nmod negative,selection
R3887 T13042 T13041 punct /,negative
R3889 T13043 T13039 compound selection,marker
R3890 T13044 T13045 dep that,preserves
R3891 T13045 T13039 relcl preserves,marker
R3892 T13046 T13047 det the,capacity
R3894 T13047 T13045 dobj capacity,preserves
R3895 T13048 T13047 compound germline,capacity
R3896 T13049 T13047 prep of,capacity
R3898 T13050 T13051 compound ES,cells
R3899 T13051 T13049 pobj cells,of
R3900 T13052 T13039 punct ", ",marker
R3902 T13053 T13039 cc and,marker
R3903 T13054 T13039 punct ", ",marker
R3906 T13055 T13056 prep for,existence
R3907 T13056 T13039 conj existence,marker
R3908 T13057 T13058 amod somatic,cells
R3910 T13058 T13055 pobj cells,for
R3911 T13059 T13056 punct ", ",existence
R3912 T13060 T13056 det the,existence
R3913 T13061 T13056 prep of,existence
R3914 T13062 T13063 det a,allele
R3915 T13063 T13061 pobj allele,of
R3917 T13064 T13063 amod knock,allele
R3918 T13065 T13064 punct -,knock
R3919 T13066 T13064 prt out,knock
R3921 T13067 T13063 prep of,allele
R3922 T13068 T13069 det the,gene
R3923 T13069 T13067 pobj gene,of
R3925 T13070 T13069 prep of,gene
R3926 T13071 T13070 pobj interest,of
R3928 T13072 T13023 punct .,relied
R3929 T13074 T13075 det These,characteristics
R3931 T13075 T13076 nsubj characteristics,make
R3932 T13077 T13076 aux should,make
R3934 T13078 T13079 compound RMCE,ASAP
R3935 T13079 T13081 nsubj ASAP,technology
R3936 T13080 T13079 punct -,ASAP
R3938 T13081 T13076 ccomp technology,make
R3939 T13082 T13081 det a,technology
R3940 T13083 T13081 amod robust,technology
R3942 T13084 T13083 cc and,robust
R3943 T13085 T13083 conj general,robust
R3944 T13086 T13081 prep for,technology
R3946 T13087 T13086 pobj analysis,for
R3947 T13088 T13087 prep of,analysis
R3948 T13089 T13090 amod mammalian,genes
R3950 T13090 T13088 pobj genes,of
R3951 T13091 T13087 prep under,analysis
R3953 T13092 T13091 pobj conditions,under
R3954 T13093 T13094 dep that,preserve
R3955 T13094 T13092 relcl preserve,conditions
R3957 T13095 T13096 amod normal,mechanisms
R3958 T13096 T13094 dobj mechanisms,preserve
R3959 T13097 T13096 compound control,mechanisms
R3960 T13098 T13094 prep in,preserve
R3962 T13099 T13100 amod different,tissues
R3963 T13100 T13098 pobj tissues,in
R3965 T13101 T13076 punct .,make
R3967 T13103 T13104 prep In,used
R3969 T13105 T13103 pobj addition,In
R3970 T13106 T13104 punct ", ",used
R3971 T13107 T13108 compound RMCE,ASAP
R3973 T13108 T13104 nsubjpass ASAP,used
R3974 T13109 T13108 punct -,ASAP
R3976 T13110 T13104 aux could,used
R3977 T13111 T13104 auxpass be,used
R3978 T13112 T13113 aux to,generate
R3980 T13113 T13104 advcl generate,used
R3981 T13114 T13115 amod fibroblastic,lines
R3982 T13115 T13113 dobj lines,generate
R3984 T13116 T13115 compound cell,lines
R3985 T13117 T13115 acl tailored,lines
R3988 T13118 T13117 prep for,tailored
R3990 T13119 T13120 det the,targeting
R3991 T13120 T13118 pobj targeting,for
R3992 T13121 T13120 amod repeated,targeting
R3994 T13122 T13120 prep of,targeting
R3995 T13123 T13124 advmod widely,studied
R3996 T13124 T13125 amod studied,genes
R3998 T13125 T13122 pobj genes,of
R3999 T13126 T13125 punct (,genes
R4000 T13127 T13125 appos p53,genes
R4002 T13128 T13127 punct ", ",p53
R4003 T13129 T13130 compound c,myc
R4004 T13130 T13127 conj myc,p53
R4006 T13131 T13130 punct -,myc
R4007 T13132 T13130 punct ", ",myc
R4008 T13133 T13134 compound NF,KB
R4010 T13134 T13130 conj KB,myc
R4011 T13135 T13134 punct -,KB
R4012 T13136 T13134 punct ", ",KB
R4014 T13137 T13134 conj etc.,KB
R4015 T13138 T13104 punct ),used
R4016 T13139 T13104 punct .,used
R4021 T12988 T12989 det These,data
R4022 T12989 T12990 nsubj data,report
R4023 T12991 T12992 det the,development
R4024 T12992 T12990 dobj development,report
R4025 T12993 T12992 cc and,development
R4026 T12994 T12992 conj implementation,development
R4027 T12995 T12992 prep of,development
R4028 T12996 T12997 det an,approach
R4029 T12997 T12995 pobj approach,of
R4030 T12998 T12999 amod improved,RMCE
R4031 T12999 T12997 compound RMCE,approach
R4032 T13000 T13001 dep that,enables
R4033 T13001 T12997 relcl enables,approach
R4034 T13002 T13003 amod efficient,modification
R4035 T13003 T13001 dobj modification,enables
R4036 T13004 T13003 compound allele,modification
R4037 T13005 T13001 prep in,enables
R4038 T13006 T13007 compound ES,cells
R4039 T13007 T13005 pobj cells,in
R4040 T13008 T13009 aux to,generate
R4041 T13009 T13001 advcl generate,enables
R4042 T13010 T13009 dobj mice,generate
R4043 T13011 T13001 cc and,enables