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PMC:1540739 / 19660-20558 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T12797 0-2 PRP denotes We
T12799 3-7 RB denotes also
T12798 8-17 VBD denotes performed
T12800 18-22 NN denotes RMCE
T12801 23-25 IN denotes in
T12802 26-33 NN denotes p53RMCE
T12804 33-34 HYPH denotes /
T12805 34-35 SYM denotes −
T12803 36-40 NNS denotes MEFs
T12806 41-45 IN denotes with
T12807 46-49 DT denotes the
T12809 50-58 NN denotes p53ΔPGFP
T12808 59-68 NN denotes construct
T12810 68-69 . denotes .
T12811 69-262 sentence denotes p53ΔPGFP-expressing MEFs were viable, recovered with an efficiency of ∼40%, and, as expected from ES cell experiments, expressed a p53ΔPGFP protein at much lower levels than p53WT (Figure 5B).
T12812 70-78 NN denotes p53ΔPGFP
T12814 78-79 HYPH denotes -
T12813 79-89 VBG denotes expressing
T12815 90-94 NNS denotes MEFs
T12816 95-99 VBD denotes were
T12817 100-106 JJ denotes viable
T12818 106-108 , denotes ,
T12819 108-117 VBD denotes recovered
T12820 118-122 IN denotes with
T12821 123-125 DT denotes an
T12822 126-136 NN denotes efficiency
T12823 137-139 IN denotes of
T12824 140-141 SYM denotes ∼
T12825 141-143 CD denotes 40
T12826 143-144 NN denotes %
T12827 144-146 , denotes ,
T12828 146-149 CC denotes and
T12829 149-151 , denotes ,
T12830 151-153 IN denotes as
T12831 154-162 VBN denotes expected
T12833 163-167 IN denotes from
T12834 168-170 NN denotes ES
T12835 171-175 NN denotes cell
T12836 176-187 NNS denotes experiments
T12837 187-189 , denotes ,
T12832 189-198 VBD denotes expressed
T12838 199-200 DT denotes a
T12840 201-209 NN denotes p53ΔPGFP
T12839 210-217 NN denotes protein
T12841 218-220 IN denotes at
T12842 221-225 RB denotes much
T12843 226-231 JJR denotes lower
T12844 232-238 NNS denotes levels
T12845 239-243 IN denotes than
T12846 244-249 NN denotes p53WT
T12847 250-251 -LRB- denotes (
T12849 251-257 NN denotes Figure
T12848 258-260 NN denotes 5B
T12850 260-261 -RRB- denotes )
T12851 261-262 . denotes .
T12852 262-344 sentence denotes Unlike WT MEFs, p53ΔP/ΔP MEFs are unable to arrest cycling after irradiation (5).
T12853 263-269 IN denotes Unlike
T12855 270-272 NN denotes WT
T12856 273-277 NNS denotes MEFs
T12857 277-279 , denotes ,
T12858 279-284 NN denotes p53ΔP
T12860 284-285 HYPH denotes /
T12859 285-287 NN denotes ΔP
T12861 288-292 NNS denotes MEFs
T12854 293-296 VBP denotes are
T12862 297-303 JJ denotes unable
T12863 304-306 TO denotes to
T12864 307-313 VB denotes arrest
T12865 314-321 VBG denotes cycling
T12866 322-327 IN denotes after
T12867 328-339 NN denotes irradiation
T12868 340-341 -LRB- denotes (
T12869 341-342 CD denotes 5
T12870 342-343 -RRB- denotes )
T12871 343-344 . denotes .
T12872 344-531 sentence denotes Likewise, we found that irradiation doses that arrested p53RMCE/− MEFs (which express a wild-type p53 from the RMCE-ready locus, see Figure 2) did not arrest p53ΔPGFP/− MEFs (Figure 5C).
T12873 345-353 RB denotes Likewise
T12875 353-355 , denotes ,
T12876 355-357 PRP denotes we
T12874 358-363 VBD denotes found
T12877 364-368 IN denotes that
T12879 369-380 NN denotes irradiation
T12880 381-386 NNS denotes doses
T12881 387-391 WDT denotes that
T12882 392-400 VBD denotes arrested
T12883 401-408 NN denotes p53RMCE
T12885 408-409 HYPH denotes /
T12886 409-410 SYM denotes −
T12884 411-415 NNS denotes MEFs
T12887 416-417 -LRB- denotes (
T12888 417-422 WDT denotes which
T12889 423-430 VBP denotes express
T12890 431-432 DT denotes a
T12892 433-437 JJ denotes wild
T12894 437-438 HYPH denotes -
T12893 438-442 NN denotes type
T12891 443-446 NN denotes p53
T12895 447-451 IN denotes from
T12896 452-455 DT denotes the
T12898 456-460 NN denotes RMCE
T12900 460-461 HYPH denotes -
T12899 461-466 JJ denotes ready
T12897 467-472 NN denotes locus
T12901 472-474 , denotes ,
T12902 474-477 VBP denotes see
T12903 478-484 NN denotes Figure
T12904 485-486 CD denotes 2
T12905 486-487 -RRB- denotes )
T12906 488-491 VBD denotes did
T12907 492-495 RB denotes not
T12878 496-502 VB denotes arrest
T12908 503-511 NN denotes p53ΔPGFP
T12910 511-512 HYPH denotes /
T12911 512-513 SYM denotes −
T12909 514-518 NNS denotes MEFs
T12912 519-520 -LRB- denotes (
T12914 520-526 NN denotes Figure
T12913 527-529 NN denotes 5C
T12915 529-530 -RRB- denotes )
T12916 530-531 . denotes .
T12917 531-645 sentence denotes These data show that a single RMCE-ASAP reaction in heterozygous MEFs enables detection of a recessive phenotype.
T12918 532-537 DT denotes These
T12919 538-542 NNS denotes data
T12920 543-547 VBP denotes show
T12921 548-552 IN denotes that
T12923 553-554 DT denotes a
T12925 555-561 JJ denotes single
T12926 562-566 NN denotes RMCE
T12928 566-567 HYPH denotes -
T12927 567-571 NN denotes ASAP
T12924 572-580 NN denotes reaction
T12929 581-583 IN denotes in
T12930 584-596 JJ denotes heterozygous
T12931 597-601 NNS denotes MEFs
T12922 602-609 VBZ denotes enables
T12932 610-619 NN denotes detection
T12933 620-622 IN denotes of
T12934 623-624 DT denotes a
T12936 625-634 JJ denotes recessive
T12935 635-644 NN denotes phenotype
T12937 644-645 . denotes .
T12938 645-848 sentence denotes The results confirm that deleting the proline rich domain leads to less active p53 with impaired cell cycle control, and also indicate that a GFP C-terminal fusion can dramatically alter p53 regulation.
T12939 646-649 DT denotes The
T12940 650-657 NNS denotes results
T12941 658-665 VBP denotes confirm
T12942 666-670 IN denotes that
T12944 671-679 VBG denotes deleting
T12945 680-683 DT denotes the
T12947 684-691 NN denotes proline
T12948 692-696 JJ denotes rich
T12946 697-703 NN denotes domain
T12943 704-709 VBZ denotes leads
T12949 710-712 IN denotes to
T12950 713-717 RBR denotes less
T12951 718-724 JJ denotes active
T12952 725-728 NN denotes p53
T12953 729-733 IN denotes with
T12954 734-742 JJ denotes impaired
T12956 743-747 NN denotes cell
T12957 748-753 NN denotes cycle
T12955 754-761 NN denotes control
T12958 761-763 , denotes ,
T12959 763-766 CC denotes and
T12960 767-771 RB denotes also
T12961 772-780 VBP denotes indicate
T12962 781-785 IN denotes that
T12964 786-787 DT denotes a
T12966 788-791 NN denotes GFP
T12967 792-793 NN denotes C
T12968 793-794 HYPH denotes -
T12969 794-802 JJ denotes terminal
T12965 803-809 NN denotes fusion
T12970 810-813 MD denotes can
T12971 814-826 RB denotes dramatically
T12963 827-832 VB denotes alter
T12972 833-836 NN denotes p53
T12973 837-847 NN denotes regulation
T12974 847-848 . denotes .
T12975 848-898 sentence denotes A summary of our results is presented in Table 1.
T12976 849-850 DT denotes A
T12977 851-858 NN denotes summary
T12979 859-861 IN denotes of
T12980 862-865 PRP$ denotes our
T12981 866-873 NNS denotes results
T12982 874-876 VBZ denotes is
T12978 877-886 VBN denotes presented
T12983 887-889 IN denotes in
T12984 890-895 NN denotes Table
T12985 896-897 CD denotes 1
T12986 897-898 . denotes .
R3705 T12797 T12798 nsubj We,performed
R3706 T12799 T12798 advmod also,performed
R3708 T12800 T12798 dobj RMCE,performed
R3711 T12801 T12798 prep in,performed
R3712 T12802 T12803 nmod p53RMCE,MEFs
R3714 T12803 T12801 pobj MEFs,in
R3715 T12804 T12802 punct /,p53RMCE
R3717 T12805 T12802 punct −,p53RMCE
R3718 T12806 T12798 prep with,performed
R3720 T12807 T12808 det the,construct
R3721 T12808 T12806 pobj construct,with
R3723 T12809 T12808 compound p53ΔPGFP,construct
R3724 T12810 T12798 punct .,performed
R3726 T12812 T12813 npadvmod p53ΔPGFP,expressing
R3727 T12813 T12815 amod expressing,MEFs
R3728 T12814 T12813 punct -,expressing
R3730 T12815 T12816 nsubj MEFs,were
R3732 T12817 T12816 acomp viable,were
R3733 T12818 T12816 punct ", ",were
R3734 T12819 T12816 conj recovered,were
R3736 T12820 T12819 prep with,recovered
R3737 T12821 T12822 det an,efficiency
R3739 T12822 T12820 pobj efficiency,with
R3740 T12823 T12822 prep of,efficiency
R3741 T12824 T12825 punct ∼,40
R3743 T12825 T12826 nummod 40,%
R3744 T12826 T12823 pobj %,of
R3745 T12827 T12819 punct ", ",recovered
R3747 T12828 T12819 cc and,recovered
R3748 T12829 T12819 punct ", ",recovered
R3749 T12830 T12831 mark as,expected
R3751 T12831 T12832 advcl expected,expressed
R3752 T12832 T12819 conj expressed,recovered
R3753 T12833 T12831 prep from,expected
R3755 T12834 T12835 compound ES,cell
R3756 T12835 T12836 compound cell,experiments
R3757 T12836 T12833 pobj experiments,from
R3758 T12837 T12832 punct ", ",expressed
R3759 T12838 T12839 det a,protein
R3761 T12839 T12832 dobj protein,expressed
R3762 T12840 T12839 compound p53ΔPGFP,protein
R3763 T12841 T12832 prep at,expressed
R3764 T12842 T12843 advmod much,lower
R3765 T12906 T12878 aux did,arrest
R3766 T12843 T12844 amod lower,levels
R3767 T12844 T12841 pobj levels,at
R3768 T12845 T12844 prep than,levels
R3769 T12846 T12845 pobj p53WT,than
R3770 T12907 T12878 neg not,arrest
R3771 T12847 T12848 punct (,5B
R3772 T12848 T12832 parataxis 5B,expressed
R3773 T12849 T12848 compound Figure,5B
R3774 T12850 T12848 punct ),5B
R3775 T12908 T12909 nmod p53ΔPGFP,MEFs
R3776 T12851 T12816 punct .,were
R3777 T12909 T12878 dobj MEFs,arrest
R3778 T12853 T12854 prep Unlike,are
R3779 T12855 T12856 compound WT,MEFs
R3780 T12910 T12908 punct /,p53ΔPGFP
R3781 T12856 T12853 pobj MEFs,Unlike
R3782 T12857 T12854 punct ", ",are
R3783 T12858 T12859 compound p53ΔP,ΔP
R3784 T12911 T12908 punct −,p53ΔPGFP
R3785 T12859 T12861 compound ΔP,MEFs
R3786 T12860 T12859 punct /,ΔP
R3787 T12912 T12913 punct (,5C
R3788 T12861 T12854 nsubj MEFs,are
R3789 T12862 T12854 acomp unable,are
R3790 T12863 T12864 aux to,arrest
R3791 T12913 T12878 parataxis 5C,arrest
R3792 T12864 T12862 xcomp arrest,unable
R3793 T12865 T12864 dobj cycling,arrest
R3794 T12866 T12864 prep after,arrest
R3795 T12914 T12913 compound Figure,5C
R3796 T12867 T12866 pobj irradiation,after
R3797 T12868 T12869 punct (,5
R3798 T12869 T12854 parataxis 5,are
R3799 T12915 T12913 punct ),5C
R3800 T12870 T12869 punct ),5
R3801 T12871 T12854 punct .,are
R3802 T12916 T12874 punct .,found
R3803 T12873 T12874 advmod Likewise,found
R3804 T12918 T12919 det These,data
R3805 T12875 T12874 punct ", ",found
R3806 T12876 T12874 nsubj we,found
R3807 T12877 T12878 mark that,arrest
R3808 T12919 T12920 nsubj data,show
R3809 T12878 T12874 ccomp arrest,found
R3810 T12921 T12922 mark that,enables
R3811 T12879 T12880 compound irradiation,doses
R3812 T12880 T12878 nsubj doses,arrest
R3813 T12881 T12882 dep that,arrested
R3814 T12882 T12880 relcl arrested,doses
R3815 T12922 T12920 ccomp enables,show
R3816 T12883 T12884 nmod p53RMCE,MEFs
R3817 T12884 T12882 dobj MEFs,arrested
R3818 T12923 T12924 det a,reaction
R3819 T12885 T12883 punct /,p53RMCE
R3820 T12886 T12883 punct −,p53RMCE
R3821 T12887 T12880 punct (,doses
R3822 T12924 T12922 nsubj reaction,enables
R3823 T12888 T12889 dep which,express
R3824 T12889 T12880 relcl express,doses
R3825 T12890 T12891 det a,p53
R3826 T12925 T12924 amod single,reaction
R3827 T12891 T12889 dobj p53,express
R3828 T12892 T12893 amod wild,type
R3829 T12926 T12927 compound RMCE,ASAP
R3830 T12893 T12891 compound type,p53
R3831 T12894 T12893 punct -,type
R3832 T12895 T12891 prep from,p53
R3833 T12927 T12924 compound ASAP,reaction
R3834 T12896 T12897 det the,locus
R3835 T12897 T12895 pobj locus,from
R3836 T12898 T12899 npadvmod RMCE,ready
R3837 T12928 T12927 punct -,ASAP
R3838 T12899 T12897 amod ready,locus
R3839 T12900 T12899 punct -,ready
R3840 T12901 T12878 punct ", ",arrest
R3841 T12929 T12924 prep in,reaction
R3842 T12902 T12878 parataxis see,arrest
R3843 T12903 T12902 dobj Figure,see
R3844 T12930 T12931 amod heterozygous,MEFs
R3845 T12904 T12903 nummod 2,Figure
R3846 T12905 T12878 punct ),arrest
R3847 T12931 T12929 pobj MEFs,in
R3848 T12932 T12922 dobj detection,enables
R3849 T12933 T12932 prep of,detection
R3851 T12934 T12935 det a,phenotype
R3854 T12935 T12933 pobj phenotype,of
R3858 T12936 T12935 amod recessive,phenotype
R3863 T12937 T12920 punct .,show
R3866 T12939 T12940 det The,results
R3872 T12940 T12941 nsubj results,confirm
R3876 T12942 T12943 mark that,leads
R3881 T12943 T12941 ccomp leads,confirm
R3884 T12944 T12943 csubj deleting,leads
R3888 T12945 T12946 det the,domain
R3893 T12946 T12944 dobj domain,deleting
R3897 T12947 T12948 npadvmod proline,rich
R3901 T12948 T12946 amod rich,domain
R3904 T12949 T12943 prep to,leads
R3905 T12950 T12951 advmod less,active
R3909 T12951 T12952 amod active,p53
R3916 T12952 T12949 pobj p53,to
R3920 T12953 T12952 prep with,p53
R3924 T12954 T12955 amod impaired,control
R3927 T12955 T12953 pobj control,with
R3930 T12956 T12957 compound cell,cycle
R3933 T12957 T12955 compound cycle,control
R3937 T12958 T12941 punct ", ",confirm
R3941 T12959 T12941 cc and,confirm
R3945 T12960 T12961 advmod also,indicate
R3949 T12961 T12941 conj indicate,confirm
R3952 T12962 T12963 mark that,alter
R3956 T12963 T12961 ccomp alter,indicate
R3961 T12964 T12965 det a,fusion
R3964 T12965 T12963 nsubj fusion,alter
R3966 T12966 T12965 nmod GFP,fusion
R3968 T12967 T12965 nmod C,fusion
R3972 T12968 T12967 punct -,C
R3975 T12969 T12967 amod terminal,C
R3979 T12970 T12963 aux can,alter
R3983 T12971 T12963 advmod dramatically,alter
R3986 T12972 T12973 compound p53,regulation
R3987 T12973 T12963 dobj regulation,alter
R3989 T12974 T12941 punct .,confirm
R3993 T12976 T12977 det A,summary
R3997 T12977 T12978 nsubjpass summary,presented
R4001 T12979 T12977 prep of,summary
R4005 T12980 T12981 poss our,results
R4009 T12981 T12979 pobj results,of
R4013 T12982 T12978 auxpass is,presented
R4017 T12983 T12978 prep in,presented
R4018 T12984 T12983 pobj Table,in
R4019 T12985 T12984 nummod 1,Table
R4020 T12986 T12978 punct .,presented