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PMC:1540739 / 17737-19659 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T12411 0-2 PRP denotes We
T12413 3-7 RB denotes next
T12412 8-18 VBD denotes determined
T12414 19-26 IN denotes whether
T12416 27-30 DT denotes the
T12418 31-35 NN denotes RMCE
T12417 36-44 NN denotes approach
T12419 45-50 MD denotes could
T12420 51-53 VB denotes be
T12415 54-58 VBN denotes used
T12421 59-61 TO denotes to
T12422 62-68 VB denotes target
T12423 69-78 NNS denotes mutations
T12424 79-81 IN denotes at
T12425 82-85 DT denotes the
T12427 86-89 NN denotes p53
T12426 90-96 NN denotes allele
T12428 97-99 IN denotes in
T12429 100-107 JJ denotes somatic
T12430 108-113 NNS denotes cells
T12431 114-115 -LRB- denotes (
T12432 115-121 NN denotes Figure
T12433 122-123 CD denotes 1
T12434 123-125 , denotes ,
T12435 125-129 NN denotes path
T12436 130-131 NN denotes B
T12437 131-132 -RRB- denotes )
T12438 132-133 . denotes .
T12439 133-296 sentence denotes We first verified that the RMCE-ready p53 locus (p53RMCE) could be transmitted through the germline by mating p53RMCE/+ chimeras with p53+/− mice (20) (Figure 4).
T12440 134-136 PRP denotes We
T12442 137-142 RB denotes first
T12441 143-151 VBD denotes verified
T12443 152-156 IN denotes that
T12445 157-160 DT denotes the
T12447 161-165 NN denotes RMCE
T12449 165-166 HYPH denotes -
T12448 166-171 JJ denotes ready
T12450 172-175 NN denotes p53
T12446 176-181 NN denotes locus
T12451 182-183 -LRB- denotes (
T12452 183-190 NN denotes p53RMCE
T12453 190-191 -RRB- denotes )
T12454 192-197 MD denotes could
T12455 198-200 VB denotes be
T12444 201-212 VBN denotes transmitted
T12456 213-220 IN denotes through
T12457 221-224 DT denotes the
T12458 225-233 NN denotes germline
T12459 234-236 IN denotes by
T12460 237-243 VBG denotes mating
T12461 244-251 NN denotes p53RMCE
T12463 251-252 HYPH denotes /
T12464 252-253 SYM denotes +
T12462 254-262 NNS denotes chimeras
T12465 263-267 IN denotes with
T12466 268-271 NN denotes p53
T12468 271-272 SYM denotes +
T12469 272-273 HYPH denotes /
T12470 273-274 SYM denotes −
T12467 275-279 NNS denotes mice
T12471 280-281 -LRB- denotes (
T12472 281-283 CD denotes 20
T12473 283-284 -RRB- denotes )
T12474 285-286 -LRB- denotes (
T12475 286-292 NN denotes Figure
T12476 293-294 CD denotes 4
T12477 294-295 -RRB- denotes )
T12478 295-296 . denotes .
T12479 296-417 sentence denotes Importantly, this allowed us to generate p53RMCE/− MEFs, which were used to test RMCE at the p53 locus in somatic cells.
T12480 297-308 RB denotes Importantly
T12482 308-310 , denotes ,
T12483 310-314 DT denotes this
T12481 315-322 VBD denotes allowed
T12484 323-325 PRP denotes us
T12486 326-328 TO denotes to
T12485 329-337 VB denotes generate
T12487 338-345 NN denotes p53RMCE
T12489 345-346 HYPH denotes /
T12490 346-347 SYM denotes −
T12488 348-352 NNS denotes MEFs
T12491 352-354 , denotes ,
T12492 354-359 WDT denotes which
T12494 360-364 VBD denotes were
T12493 365-369 VBN denotes used
T12495 370-372 TO denotes to
T12496 373-377 VB denotes test
T12497 378-382 NN denotes RMCE
T12498 383-385 IN denotes at
T12499 386-389 DT denotes the
T12501 390-393 NN denotes p53
T12500 394-399 NN denotes locus
T12502 400-402 IN denotes in
T12503 403-410 JJ denotes somatic
T12504 411-416 NNS denotes cells
T12505 416-417 . denotes .
T12506 417-585 sentence denotes We first attempted RMCE in MEFs by electroporating p53RMCE/− MEFs with a Cre-expression plasmid and the p53GFP plasmid, followed by selection with FIAU or ganciclovir.
T12507 418-420 PRP denotes We
T12509 421-426 RB denotes first
T12508 427-436 VBD denotes attempted
T12510 437-441 NN denotes RMCE
T12511 442-444 IN denotes in
T12512 445-449 NNS denotes MEFs
T12513 450-452 IN denotes by
T12514 453-468 VBG denotes electroporating
T12515 469-476 NN denotes p53RMCE
T12517 476-477 HYPH denotes /
T12518 477-478 SYM denotes −
T12516 479-483 NNS denotes MEFs
T12519 484-488 IN denotes with
T12520 489-490 DT denotes a
T12522 491-494 NN denotes Cre
T12524 494-495 HYPH denotes -
T12523 495-505 NN denotes expression
T12521 506-513 NN denotes plasmid
T12525 514-517 CC denotes and
T12526 518-521 DT denotes the
T12528 522-528 NN denotes p53GFP
T12527 529-536 NN denotes plasmid
T12529 536-538 , denotes ,
T12530 538-546 VBN denotes followed
T12531 547-549 IN denotes by
T12532 550-559 NN denotes selection
T12533 560-564 IN denotes with
T12534 565-569 NN denotes FIAU
T12535 570-572 CC denotes or
T12536 573-584 NN denotes ganciclovir
T12537 584-585 . denotes .
T12538 585-666 sentence denotes Strikingly, no clones with an exchanged allele were identified (data not shown).
T12539 586-596 RB denotes Strikingly
T12541 596-598 , denotes ,
T12542 598-600 DT denotes no
T12543 601-607 NNS denotes clones
T12544 608-612 IN denotes with
T12545 613-615 DT denotes an
T12547 616-625 VBN denotes exchanged
T12546 626-632 NN denotes allele
T12548 633-637 VBD denotes were
T12540 638-648 VBN denotes identified
T12549 649-650 -LRB- denotes (
T12551 650-654 NNS denotes data
T12552 655-658 RB denotes not
T12550 659-664 VBN denotes shown
T12553 664-665 -RRB- denotes )
T12554 665-666 . denotes .
T12555 666-869 sentence denotes RMCE with p53GFP in ES cells showed that p53GFP is expressed at high levels (Figure 3A), and as mentioned before, the p53 pathway that can be activated in MEFs is not readily activated in ES cells (18).
T12556 667-671 NN denotes RMCE
T12558 672-676 IN denotes with
T12559 677-683 NN denotes p53GFP
T12560 684-686 IN denotes in
T12561 687-689 NN denotes ES
T12562 690-695 NNS denotes cells
T12557 696-702 VBD denotes showed
T12563 703-707 IN denotes that
T12565 708-714 NN denotes p53GFP
T12566 715-717 VBZ denotes is
T12564 718-727 VBN denotes expressed
T12567 728-730 IN denotes at
T12568 731-735 JJ denotes high
T12569 736-742 NNS denotes levels
T12570 743-744 -LRB- denotes (
T12572 744-750 NN denotes Figure
T12571 751-753 NN denotes 3A
T12573 753-754 -RRB- denotes )
T12574 754-756 , denotes ,
T12575 756-759 CC denotes and
T12576 760-762 IN denotes as
T12577 763-772 VBN denotes mentioned
T12579 773-779 RB denotes before
T12580 779-781 , denotes ,
T12581 781-784 DT denotes the
T12583 785-788 NN denotes p53
T12582 789-796 NN denotes pathway
T12584 797-801 WDT denotes that
T12586 802-805 MD denotes can
T12587 806-808 VB denotes be
T12585 809-818 VBN denotes activated
T12588 819-821 IN denotes in
T12589 822-826 NNS denotes MEFs
T12590 827-829 VBZ denotes is
T12591 830-833 RB denotes not
T12592 834-841 RB denotes readily
T12578 842-851 VBN denotes activated
T12593 852-854 IN denotes in
T12594 855-857 NN denotes ES
T12595 858-863 NNS denotes cells
T12596 864-865 -LRB- denotes (
T12597 865-867 CD denotes 18
T12598 867-868 -RRB- denotes )
T12599 868-869 . denotes .
T12600 869-1038 sentence denotes The results above suggest that high levels of p53GFP could be tolerated by ES cells but toxic to MEFs, so that MEFs in which an RMCE had occurred failed to proliferate.
T12601 870-873 DT denotes The
T12602 874-881 NNS denotes results
T12604 882-887 RB denotes above
T12603 888-895 VBP denotes suggest
T12605 896-900 IN denotes that
T12607 901-905 JJ denotes high
T12608 906-912 NNS denotes levels
T12609 913-915 IN denotes of
T12610 916-922 NN denotes p53GFP
T12611 923-928 MD denotes could
T12606 929-931 VB denotes be
T12612 932-941 VBN denotes tolerated
T12613 942-944 IN denotes by
T12614 945-947 NN denotes ES
T12615 948-953 NNS denotes cells
T12616 954-957 CC denotes but
T12617 958-963 JJ denotes toxic
T12618 964-966 IN denotes to
T12619 967-971 NNS denotes MEFs
T12620 971-973 , denotes ,
T12621 973-975 IN denotes so
T12623 976-980 IN denotes that
T12624 981-985 NNS denotes MEFs
T12625 986-988 IN denotes in
T12627 989-994 WDT denotes which
T12628 995-997 DT denotes an
T12629 998-1002 NN denotes RMCE
T12630 1003-1006 VBD denotes had
T12626 1007-1015 VBN denotes occurred
T12622 1016-1022 VBD denotes failed
T12631 1023-1025 TO denotes to
T12632 1026-1037 VB denotes proliferate
T12633 1037-1038 . denotes .
T12634 1038-1248 sentence denotes To test this possibility, p53RMCE/− MEFs were electroporated with the p53GFP replacement construct with or without a Cre-expression plasmid, then analyzed by fluorescence microscopy 48 h after electroporation.
T12635 1039-1041 TO denotes To
T12636 1042-1046 VB denotes test
T12638 1047-1051 DT denotes this
T12639 1052-1063 NN denotes possibility
T12640 1063-1065 , denotes ,
T12641 1065-1072 NN denotes p53RMCE
T12643 1072-1073 HYPH denotes /
T12644 1073-1074 SYM denotes −
T12642 1075-1079 NNS denotes MEFs
T12645 1080-1084 VBD denotes were
T12637 1085-1099 VBN denotes electroporated
T12646 1100-1104 IN denotes with
T12647 1105-1108 DT denotes the
T12649 1109-1115 NN denotes p53GFP
T12650 1116-1127 NN denotes replacement
T12648 1128-1137 NN denotes construct
T12651 1138-1142 IN denotes with
T12652 1143-1145 CC denotes or
T12653 1146-1153 IN denotes without
T12654 1154-1155 DT denotes a
T12656 1156-1159 NN denotes Cre
T12658 1159-1160 HYPH denotes -
T12657 1160-1170 NN denotes expression
T12655 1171-1178 NN denotes plasmid
T12659 1178-1180 , denotes ,
T12660 1180-1184 RB denotes then
T12661 1185-1193 VBN denotes analyzed
T12662 1194-1196 IN denotes by
T12663 1197-1209 NN denotes fluorescence
T12664 1210-1220 NN denotes microscopy
T12665 1221-1223 CD denotes 48
T12666 1224-1225 NN denotes h
T12667 1226-1231 IN denotes after
T12668 1232-1247 NN denotes electroporation
T12669 1247-1248 . denotes .
T12670 1248-1466 sentence denotes The experiment was done without selection to enable observation of cells under conditions where a failure to proliferate would not derive from FIAU or ganciclovir toxicity but rather solely from the effects of p53GFP.
T12671 1249-1252 DT denotes The
T12672 1253-1263 NN denotes experiment
T12674 1264-1267 VBD denotes was
T12673 1268-1272 VBN denotes done
T12675 1273-1280 IN denotes without
T12676 1281-1290 NN denotes selection
T12677 1291-1293 TO denotes to
T12678 1294-1300 VB denotes enable
T12679 1301-1312 NN denotes observation
T12680 1313-1315 IN denotes of
T12681 1316-1321 NNS denotes cells
T12682 1322-1327 IN denotes under
T12683 1328-1338 NNS denotes conditions
T12684 1339-1344 WRB denotes where
T12686 1345-1346 DT denotes a
T12687 1347-1354 NN denotes failure
T12688 1355-1357 TO denotes to
T12689 1358-1369 VB denotes proliferate
T12690 1370-1375 MD denotes would
T12691 1376-1379 RB denotes not
T12685 1380-1386 VB denotes derive
T12692 1387-1391 IN denotes from
T12693 1392-1396 NN denotes FIAU
T12695 1397-1399 CC denotes or
T12696 1400-1411 NN denotes ganciclovir
T12694 1412-1420 NN denotes toxicity
T12697 1421-1424 CC denotes but
T12698 1425-1431 RB denotes rather
T12699 1432-1438 RB denotes solely
T12700 1439-1443 IN denotes from
T12701 1444-1447 DT denotes the
T12702 1448-1455 NNS denotes effects
T12703 1456-1458 IN denotes of
T12704 1459-1465 NN denotes p53GFP
T12705 1465-1466 . denotes .
T12706 1466-1609 sentence denotes We observed a few fluorescent cells only when the Cre expression plasmid was co-electroporated, suggesting that such cells resulted from RMCE.
T12707 1467-1469 PRP denotes We
T12708 1470-1478 VBD denotes observed
T12709 1479-1480 DT denotes a
T12711 1481-1484 JJ denotes few
T12712 1485-1496 JJ denotes fluorescent
T12710 1497-1502 NNS denotes cells
T12713 1503-1507 RB denotes only
T12714 1508-1512 WRB denotes when
T12716 1513-1516 DT denotes the
T12718 1517-1520 NN denotes Cre
T12719 1521-1531 NN denotes expression
T12717 1532-1539 NN denotes plasmid
T12720 1540-1543 VBD denotes was
T12715 1544-1561 VBN denotes co-electroporated
T12721 1561-1563 , denotes ,
T12722 1563-1573 VBG denotes suggesting
T12723 1574-1578 IN denotes that
T12725 1579-1583 JJ denotes such
T12726 1584-1589 NNS denotes cells
T12724 1590-1598 VBD denotes resulted
T12727 1599-1603 IN denotes from
T12728 1604-1608 NN denotes RMCE
T12729 1608-1609 . denotes .
T12730 1609-1792 sentence denotes Importantly, the rare fluorescent cells had a flat, ‘fried-egg’ appearance typical of senescent cells (Figure 5A), and when plates were observed 5 days later, the cells had detached.
T12731 1610-1621 RB denotes Importantly
T12733 1621-1623 , denotes ,
T12734 1623-1626 DT denotes the
T12736 1627-1631 JJ denotes rare
T12737 1632-1643 JJ denotes fluorescent
T12735 1644-1649 NNS denotes cells
T12732 1650-1653 VBD denotes had
T12738 1654-1655 DT denotes a
T12740 1656-1660 JJ denotes flat
T12741 1660-1662 , denotes ,
T12742 1662-1663 `` denotes ‘
T12743 1663-1668 JJ denotes fried
T12745 1668-1669 HYPH denotes -
T12744 1669-1672 NN denotes egg
T12746 1672-1673 '' denotes ’
T12739 1674-1684 NN denotes appearance
T12747 1685-1692 JJ denotes typical
T12748 1693-1695 IN denotes of
T12749 1696-1705 JJ denotes senescent
T12750 1706-1711 NNS denotes cells
T12751 1712-1713 -LRB- denotes (
T12753 1713-1719 NN denotes Figure
T12752 1720-1722 NN denotes 5A
T12754 1722-1723 -RRB- denotes )
T12755 1723-1725 , denotes ,
T12756 1725-1728 CC denotes and
T12757 1729-1733 WRB denotes when
T12759 1734-1740 NNS denotes plates
T12760 1741-1745 VBD denotes were
T12758 1746-1754 VBN denotes observed
T12762 1755-1756 CD denotes 5
T12763 1757-1761 NNS denotes days
T12764 1762-1767 RB denotes later
T12765 1767-1769 , denotes ,
T12766 1769-1772 DT denotes the
T12767 1773-1778 NNS denotes cells
T12768 1779-1782 VBD denotes had
T12761 1783-1791 VBN denotes detached
T12769 1791-1792 . denotes .
T12770 1792-1922 sentence denotes Altogether, the results suggest that RMCE can give rise to p53GFP-expressing MEFs, but they die rapidly owing to p53GFP toxicity.
T12771 1793-1803 RB denotes Altogether
T12773 1803-1805 , denotes ,
T12774 1805-1808 DT denotes the
T12775 1809-1816 NNS denotes results
T12772 1817-1824 VBP denotes suggest
T12776 1825-1829 IN denotes that
T12778 1830-1834 NN denotes RMCE
T12779 1835-1838 MD denotes can
T12777 1839-1843 VB denotes give
T12780 1844-1848 NN denotes rise
T12781 1849-1851 IN denotes to
T12782 1852-1858 NN denotes p53GFP
T12784 1858-1859 HYPH denotes -
T12783 1859-1869 VBG denotes expressing
T12785 1870-1874 NNS denotes MEFs
T12786 1874-1876 , denotes ,
T12787 1876-1879 CC denotes but
T12788 1880-1884 PRP denotes they
T12789 1885-1888 VBP denotes die
T12790 1889-1896 RB denotes rapidly
T12791 1897-1902 VBG denotes owing
T12792 1903-1905 IN denotes to
T12793 1906-1912 NN denotes p53GFP
T12794 1913-1921 NN denotes toxicity
T12795 1921-1922 . denotes .
R3342 T12411 T12412 nsubj We,determined
R3344 T12413 T12412 advmod next,determined
R3345 T12414 T12415 mark whether,used
R3346 T12415 T12412 ccomp used,determined
R3348 T12416 T12417 det the,approach
R3349 T12417 T12415 nsubjpass approach,used
R3350 T12418 T12417 compound RMCE,approach
R3352 T12419 T12415 aux could,used
R3353 T12420 T12415 auxpass be,used
R3354 T12421 T12422 aux to,target
R3356 T12422 T12415 advcl target,used
R3357 T12423 T12422 dobj mutations,target
R3358 T12424 T12423 prep at,mutations
R3360 T12425 T12426 det the,allele
R3361 T12426 T12424 pobj allele,at
R3362 T12427 T12426 compound p53,allele
R3364 T12428 T12423 prep in,mutations
R3365 T12429 T12430 amod somatic,cells
R3366 T12430 T12428 pobj cells,in
R3368 T12431 T12432 punct (,Figure
R3369 T12432 T12415 parataxis Figure,used
R3370 T12433 T12432 nummod 1,Figure
R3372 T12434 T12432 punct ", ",Figure
R3373 T12435 T12436 compound path,B
R3374 T12436 T12432 appos B,Figure
R3376 T12437 T12432 punct ),Figure
R3377 T12438 T12412 punct .,determined
R3379 T12440 T12441 nsubj We,verified
R3381 T12442 T12441 advmod first,verified
R3382 T12443 T12444 mark that,transmitted
R3385 T12444 T12441 ccomp transmitted,verified
R3386 T12445 T12446 det the,locus
R3388 T12446 T12444 nsubjpass locus,transmitted
R3389 T12447 T12448 npadvmod RMCE,ready
R3391 T12448 T12446 amod ready,locus
R3392 T12449 T12448 punct -,ready
R3393 T12450 T12446 compound p53,locus
R3394 T12451 T12446 punct (,locus
R3395 T12452 T12446 appos p53RMCE,locus
R3396 T12453 T12444 punct ),transmitted
R3398 T12454 T12444 aux could,transmitted
R3399 T12455 T12444 auxpass be,transmitted
R3400 T12456 T12444 prep through,transmitted
R3402 T12457 T12458 det the,germline
R3403 T12458 T12456 pobj germline,through
R3404 T12459 T12444 prep by,transmitted
R3406 T12460 T12459 pcomp mating,by
R3407 T12461 T12462 nmod p53RMCE,chimeras
R3408 T12483 T12481 nsubj this,allowed
R3409 T12462 T12460 dobj chimeras,mating
R3410 T12463 T12461 punct /,p53RMCE
R3411 T12464 T12461 punct +,p53RMCE
R3412 T12465 T12460 prep with,mating
R3413 T12466 T12467 nmod p53,mice
R3414 T12484 T12485 nsubj us,generate
R3415 T12467 T12465 pobj mice,with
R3416 T12468 T12466 punct +,p53
R3417 T12469 T12466 punct /,p53
R3418 T12485 T12481 ccomp generate,allowed
R3419 T12470 T12466 punct −,p53
R3420 T12471 T12472 punct (,20
R3421 T12472 T12444 parataxis 20,transmitted
R3422 T12486 T12485 aux to,generate
R3423 T12473 T12472 punct ),20
R3424 T12474 T12475 punct (,Figure
R3425 T12475 T12441 parataxis Figure,verified
R3426 T12487 T12488 nmod p53RMCE,MEFs
R3427 T12476 T12475 nummod 4,Figure
R3428 T12477 T12475 punct ),Figure
R3429 T12478 T12441 punct .,verified
R3430 T12488 T12485 dobj MEFs,generate
R3431 T12480 T12481 advmod Importantly,allowed
R3432 T12489 T12487 punct /,p53RMCE
R3433 T12482 T12481 punct ", ",allowed
R3434 T12490 T12487 punct −,p53RMCE
R3435 T12491 T12488 punct ", ",MEFs
R3436 T12492 T12493 dep which,used
R3437 T12493 T12488 relcl used,MEFs
R3438 T12589 T12588 pobj MEFs,in
R3439 T12590 T12578 auxpass is,activated
R3440 T12494 T12493 auxpass were,used
R3441 T12591 T12578 neg not,activated
R3442 T12592 T12578 advmod readily,activated
R3443 T12593 T12578 prep in,activated
R3444 T12594 T12595 compound ES,cells
R3445 T12595 T12593 pobj cells,in
R3446 T12596 T12597 punct (,18
R3447 T12495 T12496 aux to,test
R3448 T12597 T12578 parataxis 18,activated
R3449 T12598 T12597 punct ),18
R3450 T12599 T12578 punct .,activated
R3451 T12496 T12493 advcl test,used
R3452 T12601 T12602 det The,results
R3453 T12497 T12496 dobj RMCE,test
R3454 T12602 T12603 nsubj results,suggest
R3455 T12604 T12602 advmod above,results
R3456 T12498 T12496 prep at,test
R3457 T12605 T12606 mark that,be
R3458 T12606 T12603 ccomp be,suggest
R3459 T12607 T12608 amod high,levels
R3460 T12499 T12500 det the,locus
R3461 T12608 T12606 nsubj levels,be
R3462 T12609 T12608 prep of,levels
R3463 T12500 T12498 pobj locus,at
R3464 T12610 T12609 pobj p53GFP,of
R3465 T12611 T12606 aux could,be
R3466 T12612 T12606 dep tolerated,be
R3467 T12501 T12500 compound p53,locus
R3468 T12613 T12612 agent by,tolerated
R3469 T12614 T12615 compound ES,cells
R3470 T12615 T12613 pobj cells,by
R3471 T12616 T12612 cc but,tolerated
R3472 T12502 T12496 prep in,test
R3473 T12617 T12612 conj toxic,tolerated
R3474 T12618 T12617 prep to,toxic
R3475 T12503 T12504 amod somatic,cells
R3476 T12619 T12618 pobj MEFs,to
R3477 T12620 T12606 punct ", ",be
R3478 T12621 T12622 mark so,failed
R3479 T12504 T12502 pobj cells,in
R3480 T12622 T12606 advcl failed,be
R3481 T12505 T12481 punct .,allowed
R3482 T12623 T12622 mark that,failed
R3483 T12624 T12622 nsubj MEFs,failed
R3484 T12507 T12508 nsubj We,attempted
R3485 T12625 T12626 prep in,occurred
R3486 T12626 T12624 advcl occurred,MEFs
R3487 T12627 T12625 pobj which,in
R3488 T12628 T12629 det an,RMCE
R3489 T12509 T12508 advmod first,attempted
R3490 T12629 T12626 nsubj RMCE,occurred
R3491 T12630 T12626 aux had,occurred
R3492 T12631 T12632 aux to,proliferate
R3493 T12632 T12622 xcomp proliferate,failed
R3494 T12510 T12508 dobj RMCE,attempted
R3495 T12633 T12603 punct .,suggest
R3496 T12635 T12636 aux To,test
R3497 T12636 T12637 advcl test,electroporated
R3498 T12511 T12508 prep in,attempted
R3499 T12638 T12639 det this,possibility
R3500 T12639 T12636 dobj possibility,test
R3501 T12640 T12637 punct ", ",electroporated
R3502 T12512 T12511 pobj MEFs,in
R3503 T12641 T12642 nmod p53RMCE,MEFs
R3504 T12642 T12637 nsubjpass MEFs,electroporated
R3505 T12643 T12641 punct /,p53RMCE
R3506 T12513 T12508 prep by,attempted
R3507 T12644 T12641 punct −,p53RMCE
R3508 T12645 T12637 auxpass were,electroporated
R3509 T12646 T12637 prep with,electroporated
R3510 T12514 T12513 pcomp electroporating,by
R3511 T12647 T12648 det the,construct
R3512 T12648 T12646 pobj construct,with
R3513 T12649 T12648 compound p53GFP,construct
R3514 T12515 T12516 nmod p53RMCE,MEFs
R3515 T12650 T12648 compound replacement,construct
R3516 T12651 T12637 prep with,electroporated
R3517 T12516 T12514 dobj MEFs,electroporating
R3518 T12652 T12651 cc or,with
R3519 T12653 T12651 conj without,with
R3520 T12654 T12655 det a,plasmid
R3521 T12517 T12515 punct /,p53RMCE
R3522 T12655 T12653 pobj plasmid,without
R3523 T12656 T12657 compound Cre,expression
R3524 T12657 T12655 compound expression,plasmid
R3525 T12518 T12515 punct −,p53RMCE
R3526 T12658 T12657 punct -,expression
R3527 T12659 T12637 punct ", ",electroporated
R3528 T12660 T12661 advmod then,analyzed
R3529 T12661 T12637 dep analyzed,electroporated
R3530 T12519 T12514 prep with,electroporating
R3531 T12662 T12661 prep by,analyzed
R3532 T12663 T12664 compound fluorescence,microscopy
R3533 T12520 T12521 det a,plasmid
R3534 T12664 T12662 pobj microscopy,by
R3535 T12665 T12666 nummod 48,h
R3536 T12666 T12667 npadvmod h,after
R3537 T12521 T12519 pobj plasmid,with
R3538 T12667 T12661 prep after,analyzed
R3539 T12668 T12667 pobj electroporation,after
R3540 T12669 T12637 punct .,electroporated
R3541 T12522 T12523 compound Cre,expression
R3542 T12671 T12672 det The,experiment
R3543 T12672 T12673 nsubjpass experiment,done
R3544 T12523 T12521 compound expression,plasmid
R3545 T12674 T12673 auxpass was,done
R3546 T12675 T12673 prep without,done
R3547 T12524 T12523 punct -,expression
R3548 T12676 T12675 pobj selection,without
R3549 T12677 T12678 aux to,enable
R3550 T12678 T12673 advcl enable,done
R3551 T12679 T12678 dobj observation,enable
R3552 T12680 T12679 prep of,observation
R3553 T12525 T12521 cc and,plasmid
R3554 T12681 T12680 pobj cells,of
R3555 T12682 T12678 prep under,enable
R3556 T12526 T12527 det the,plasmid
R3557 T12683 T12682 pobj conditions,under
R3558 T12684 T12685 advmod where,derive
R3559 T12685 T12683 relcl derive,conditions
R3560 T12686 T12687 det a,failure
R3561 T12527 T12521 conj plasmid,plasmid
R3562 T12687 T12685 nsubj failure,derive
R3563 T12688 T12689 aux to,proliferate
R3564 T12528 T12527 compound p53GFP,plasmid
R3565 T12689 T12687 acl proliferate,failure
R3566 T12690 T12685 aux would,derive
R3567 T12691 T12685 neg not,derive
R3568 T12529 T12521 punct ", ",plasmid
R3569 T12692 T12685 prep from,derive
R3570 T12693 T12694 nmod FIAU,toxicity
R3571 T12694 T12692 pobj toxicity,from
R3572 T12530 T12521 acl followed,plasmid
R3573 T12531 T12530 prep by,followed
R3574 T12532 T12531 pobj selection,by
R3575 T12695 T12693 cc or,FIAU
R3576 T12533 T12532 prep with,selection
R3577 T12696 T12693 conj ganciclovir,FIAU
R3578 T12697 T12692 cc but,from
R3579 T12698 T12697 advmod rather,but
R3580 T12534 T12533 pobj FIAU,with
R3581 T12699 T12700 advmod solely,from
R3582 T12700 T12692 conj from,from
R3583 T12701 T12702 det the,effects
R3584 T12535 T12534 cc or,FIAU
R3585 T12702 T12700 pobj effects,from
R3586 T12703 T12702 prep of,effects
R3587 T12536 T12534 conj ganciclovir,FIAU
R3588 T12704 T12703 pobj p53GFP,of
R3589 T12705 T12673 punct .,done
R3590 T12537 T12508 punct .,attempted
R3591 T12707 T12708 nsubj We,observed
R3592 T12539 T12540 advmod Strikingly,identified
R3593 T12709 T12710 det a,cells
R3594 T12710 T12708 dobj cells,observed
R3595 T12541 T12540 punct ", ",identified
R3596 T12711 T12710 amod few,cells
R3597 T12542 T12543 det no,clones
R3598 T12712 T12710 amod fluorescent,cells
R3599 T12713 T12714 advmod only,when
R3600 T12714 T12715 advmod when,co-electroporated
R3601 T12715 T12710 advcl co-electroporated,cells
R3602 T12543 T12540 nsubjpass clones,identified
R3603 T12716 T12717 det the,plasmid
R3604 T12717 T12715 nsubjpass plasmid,co-electroporated
R3605 T12718 T12719 compound Cre,expression
R3606 T12544 T12543 prep with,clones
R3607 T12719 T12717 compound expression,plasmid
R3608 T12720 T12715 auxpass was,co-electroporated
R3609 T12721 T12715 punct ", ",co-electroporated
R3610 T12545 T12546 det an,allele
R3611 T12722 T12715 advcl suggesting,co-electroporated
R3612 T12723 T12724 mark that,resulted
R3613 T12724 T12722 ccomp resulted,suggesting
R3614 T12546 T12544 pobj allele,with
R3615 T12725 T12726 amod such,cells
R3616 T12726 T12724 nsubj cells,resulted
R3617 T12727 T12724 prep from,resulted
R3618 T12547 T12546 amod exchanged,allele
R3619 T12728 T12727 pobj RMCE,from
R3620 T12729 T12708 punct .,observed
R3621 T12548 T12540 auxpass were,identified
R3622 T12731 T12732 advmod Importantly,had
R3623 T12733 T12732 punct ", ",had
R3624 T12549 T12550 punct (,shown
R3625 T12734 T12735 det the,cells
R3626 T12735 T12732 nsubj cells,had
R3627 T12736 T12735 amod rare,cells
R3628 T12550 T12540 parataxis shown,identified
R3629 T12737 T12735 amod fluorescent,cells
R3630 T12738 T12739 det a,appearance
R3631 T12739 T12732 dobj appearance,had
R3632 T12551 T12550 nsubj data,shown
R3633 T12740 T12739 amod flat,appearance
R3634 T12741 T12739 punct ", ",appearance
R3635 T12742 T12739 punct ‘,appearance
R3636 T12552 T12550 neg not,shown
R3637 T12743 T12744 amod fried,egg
R3638 T12744 T12739 nmod egg,appearance
R3639 T12745 T12744 punct -,egg
R3640 T12553 T12550 punct ),shown
R3641 T12746 T12739 punct ’,appearance
R3642 T12747 T12739 amod typical,appearance
R3643 T12748 T12747 prep of,typical
R3644 T12554 T12540 punct .,identified
R3645 T12749 T12750 amod senescent,cells
R3646 T12750 T12748 pobj cells,of
R3647 T12751 T12752 punct (,5A
R3648 T12556 T12557 nsubj RMCE,showed
R3649 T12752 T12732 parataxis 5A,had
R3650 T12753 T12752 compound Figure,5A
R3651 T12754 T12752 punct ),5A
R3652 T12755 T12732 punct ", ",had
R3653 T12756 T12732 cc and,had
R3654 T12757 T12758 advmod when,observed
R3655 T12758 T12761 advcl observed,detached
R3656 T12558 T12556 prep with,RMCE
R3657 T12759 T12758 nsubjpass plates,observed
R3658 T12760 T12758 auxpass were,observed
R3659 T12761 T12732 conj detached,had
R3660 T12762 T12763 nummod 5,days
R3661 T12559 T12558 pobj p53GFP,with
R3662 T12763 T12764 npadvmod days,later
R3663 T12764 T12758 advmod later,observed
R3664 T12765 T12761 punct ", ",detached
R3665 T12560 T12556 prep in,RMCE
R3666 T12766 T12767 det the,cells
R3667 T12767 T12761 nsubj cells,detached
R3668 T12561 T12562 compound ES,cells
R3669 T12768 T12761 aux had,detached
R3670 T12769 T12761 punct .,detached
R3671 T12562 T12560 pobj cells,in
R3672 T12771 T12772 advmod Altogether,suggest
R3673 T12773 T12772 punct ", ",suggest
R3674 T12563 T12564 mark that,expressed
R3675 T12774 T12775 det the,results
R3676 T12775 T12772 nsubj results,suggest
R3677 T12776 T12777 mark that,give
R3678 T12564 T12557 ccomp expressed,showed
R3679 T12777 T12772 ccomp give,suggest
R3680 T12778 T12777 nsubj RMCE,give
R3681 T12779 T12777 aux can,give
R3682 T12565 T12564 nsubjpass p53GFP,expressed
R3683 T12780 T12777 dobj rise,give
R3684 T12781 T12777 prep to,give
R3685 T12782 T12783 npadvmod p53GFP,expressing
R3686 T12566 T12564 auxpass is,expressed
R3687 T12783 T12785 amod expressing,MEFs
R3688 T12784 T12783 punct -,expressing
R3689 T12567 T12564 prep at,expressed
R3690 T12785 T12781 pobj MEFs,to
R3691 T12786 T12777 punct ", ",give
R3692 T12787 T12777 cc but,give
R3693 T12788 T12789 nsubj they,die
R3694 T12568 T12569 amod high,levels
R3695 T12789 T12777 conj die,give
R3696 T12790 T12789 advmod rapidly,die
R3697 T12569 T12567 pobj levels,at
R3698 T12791 T12789 prep owing,die
R3699 T12792 T12791 prep to,owing
R3700 T12793 T12794 compound p53GFP,toxicity
R3701 T12570 T12571 punct (,3A
R3702 T12794 T12792 pobj toxicity,to
R3703 T12795 T12772 punct .,suggest
R3704 T12571 T12564 parataxis 3A,expressed
R3707 T12572 T12571 compound Figure,3A
R3709 T12573 T12571 punct ),3A
R3710 T12574 T12557 punct ", ",showed
R3713 T12575 T12557 cc and,showed
R3716 T12576 T12577 mark as,mentioned
R3719 T12577 T12578 advcl mentioned,activated
R3722 T12578 T12557 conj activated,showed
R3725 T12579 T12577 advmod before,mentioned
R3729 T12580 T12578 punct ", ",activated
R3731 T12581 T12582 det the,pathway
R3735 T12582 T12578 nsubjpass pathway,activated
R3738 T12583 T12582 compound p53,pathway
R3742 T12584 T12585 dep that,activated
R3746 T12585 T12582 relcl activated,pathway
R3750 T12586 T12585 aux can,activated
R3754 T12587 T12585 auxpass be,activated
R3760 T12588 T12585 prep in,activated