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PMC:1540739 / 16527-17736 JSONTXT 5 Projects

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Id Subject Object Predicate Lexical cue
T12164 0-2 TO denotes To
T12165 3-7 VB denotes test
T12167 8-12 DT denotes this
T12168 13-24 NN denotes possibility
T12169 24-26 , denotes ,
T12170 26-28 PRP denotes we
T12166 29-38 VBD denotes performed
T12171 39-43 NN denotes RMCE
T12172 44-48 IN denotes with
T12173 49-50 DT denotes a
T12175 51-54 NN denotes p53
T12176 55-61 NN denotes fusion
T12174 62-66 NN denotes gene
T12177 67-69 IN denotes in
T12179 70-75 WDT denotes which
T12180 76-79 DT denotes the
T12182 80-83 NN denotes p53
T12183 84-91 NN denotes proline
T12185 91-92 HYPH denotes -
T12184 92-96 JJ denotes rich
T12181 97-103 NN denotes domain
T12186 104-105 -LRB- denotes (
T12187 105-108 NN denotes PRD
T12188 108-109 -RRB- denotes )
T12189 110-113 VBD denotes was
T12178 114-121 VBN denotes deleted
T12190 122-123 -LRB- denotes (
T12191 123-128 NN denotes p53ΔP
T12192 128-129 -RRB- denotes )
T12193 129-131 , denotes ,
T12194 131-134 CC denotes and
T12195 135-138 DT denotes the
T12196 139-144 NN denotes p53ΔP
T12198 145-148 VBD denotes was
T12197 149-154 VBN denotes fused
T12199 155-157 IN denotes to
T12200 158-161 NN denotes GFP
T12201 161-162 . denotes .
T12202 162-293 sentence denotes We used this mutant because deleting the proline-rich domain decreases stability and compromises DNA-damage responses in vivo (5).
T12203 163-165 PRP denotes We
T12204 166-170 VBD denotes used
T12205 171-175 DT denotes this
T12206 176-182 NN denotes mutant
T12207 183-190 IN denotes because
T12209 191-199 VBG denotes deleting
T12210 200-203 DT denotes the
T12212 204-211 NN denotes proline
T12214 211-212 HYPH denotes -
T12213 212-216 JJ denotes rich
T12211 217-223 NN denotes domain
T12208 224-233 VBZ denotes decreases
T12215 234-243 NN denotes stability
T12216 244-247 CC denotes and
T12217 248-259 VBZ denotes compromises
T12218 260-263 NN denotes DNA
T12220 263-264 HYPH denotes -
T12219 264-270 NN denotes damage
T12221 271-280 NNS denotes responses
T12222 281-283 FW denotes in
T12223 284-288 FW denotes vivo
T12224 289-290 -LRB- denotes (
T12225 290-291 CD denotes 5
T12226 291-292 -RRB- denotes )
T12227 292-293 . denotes .
T12228 293-388 sentence denotes According to our hypothesis, this hypomorphic mutant should not prevent embryonic development.
T12229 294-303 VBG denotes According
T12231 304-306 IN denotes to
T12232 307-310 PRP$ denotes our
T12233 311-321 NN denotes hypothesis
T12234 321-323 , denotes ,
T12235 323-327 DT denotes this
T12237 328-339 JJ denotes hypomorphic
T12236 340-346 NN denotes mutant
T12238 347-353 MD denotes should
T12239 354-357 RB denotes not
T12230 358-365 VB denotes prevent
T12240 366-375 JJ denotes embryonic
T12241 376-387 NN denotes development
T12242 387-388 . denotes .
T12243 388-575 sentence denotes RMCE with a p53ΔPGFP replacement plasmid was again very efficient and western blots revealed an additional band of the predicted molecular weight only in p53+/ΔPGFP ES cells (Figure 3B).
T12244 389-393 NN denotes RMCE
T12246 394-398 IN denotes with
T12247 399-400 DT denotes a
T12249 401-409 NN denotes p53ΔPGFP
T12250 410-421 NN denotes replacement
T12248 422-429 NN denotes plasmid
T12245 430-433 VBD denotes was
T12251 434-439 RB denotes again
T12252 440-444 RB denotes very
T12253 445-454 JJ denotes efficient
T12254 455-458 CC denotes and
T12255 459-466 NNP denotes western
T12256 467-472 NNS denotes blots
T12257 473-481 VBD denotes revealed
T12258 482-484 DT denotes an
T12260 485-495 JJ denotes additional
T12259 496-500 NN denotes band
T12261 501-503 IN denotes of
T12262 504-507 DT denotes the
T12264 508-517 VBN denotes predicted
T12265 518-527 JJ denotes molecular
T12263 528-534 NN denotes weight
T12266 535-539 RB denotes only
T12267 540-542 IN denotes in
T12268 543-546 NN denotes p53
T12270 546-547 SYM denotes +
T12271 547-548 HYPH denotes /
T12269 548-553 NN denotes ΔPGFP
T12273 554-556 NN denotes ES
T12272 557-562 NNS denotes cells
T12274 563-564 -LRB- denotes (
T12276 564-570 NN denotes Figure
T12275 571-573 NN denotes 3B
T12277 573-574 -RRB- denotes )
T12278 574-575 . denotes .
T12279 575-727 sentence denotes As expected, the PRD deletion correlated with lower expression levels: p53ΔPGFP was much less abundant than p53WT in all p53+/ΔPGFP clones (Figure 3B).
T12280 576-578 IN denotes As
T12281 579-587 VBN denotes expected
T12283 587-589 , denotes ,
T12284 589-592 DT denotes the
T12286 593-596 NN denotes PRD
T12285 597-605 NN denotes deletion
T12282 606-616 VBD denotes correlated
T12288 617-621 IN denotes with
T12289 622-627 JJR denotes lower
T12291 628-638 NN denotes expression
T12290 639-645 NNS denotes levels
T12292 645-647 : denotes :
T12293 647-655 NN denotes p53ΔPGFP
T12287 656-659 VBD denotes was
T12294 660-664 RB denotes much
T12295 665-669 RBR denotes less
T12296 670-678 JJ denotes abundant
T12297 679-683 IN denotes than
T12298 684-689 NN denotes p53WT
T12299 690-692 IN denotes in
T12300 693-696 DT denotes all
T12302 697-700 NN denotes p53
T12304 700-701 SYM denotes +
T12305 701-702 HYPH denotes /
T12303 702-707 NN denotes ΔPGFP
T12301 708-714 NNS denotes clones
T12306 715-716 -LRB- denotes (
T12308 716-722 NN denotes Figure
T12307 723-725 NN denotes 3B
T12309 725-726 -RRB- denotes )
T12310 726-727 . denotes .
T12311 727-858 sentence denotes We next determined whether p53+/ΔPGFP ES cells could generate chimeric mice and transmit the modified allele through the germline.
T12312 728-730 PRP denotes We
T12314 731-735 RB denotes next
T12313 736-746 VBD denotes determined
T12315 747-754 IN denotes whether
T12317 755-758 NN denotes p53
T12319 758-759 SYM denotes +
T12320 759-760 HYPH denotes /
T12318 760-765 NN denotes ΔPGFP
T12322 766-768 NN denotes ES
T12321 769-774 NNS denotes cells
T12323 775-780 MD denotes could
T12316 781-789 VB denotes generate
T12324 790-798 JJ denotes chimeric
T12325 799-803 NNS denotes mice
T12326 804-807 CC denotes and
T12327 808-816 VB denotes transmit
T12328 817-820 DT denotes the
T12330 821-829 VBN denotes modified
T12329 830-836 NN denotes allele
T12331 837-844 IN denotes through
T12332 845-848 DT denotes the
T12333 849-857 NN denotes germline
T12334 857-858 . denotes .
T12335 858-966 sentence denotes Two p53+/ΔPGFP ES cell clones were injected into blastocysts and highly chimeric (>80%) mice were obtained.
T12336 859-862 CD denotes Two
T12338 863-866 NN denotes p53
T12340 866-867 SYM denotes +
T12341 867-868 HYPH denotes /
T12339 868-873 NN denotes ΔPGFP
T12342 874-876 NN denotes ES
T12343 877-881 NN denotes cell
T12337 882-888 NNS denotes clones
T12345 889-893 VBD denotes were
T12344 894-902 VBN denotes injected
T12346 903-907 IN denotes into
T12347 908-919 NNS denotes blastocysts
T12348 920-923 CC denotes and
T12349 924-930 RB denotes highly
T12350 931-939 JJ denotes chimeric
T12352 940-941 -LRB- denotes (
T12353 941-942 SYM denotes >
T12354 942-944 CD denotes 80
T12351 944-945 NN denotes %
T12356 945-946 -RRB- denotes )
T12355 947-951 NNS denotes mice
T12358 952-956 VBD denotes were
T12357 957-965 VBN denotes obtained
T12359 965-966 . denotes .
T12360 966-1054 sentence denotes Heterozygote pups were recovered from the mating the chimeras with WT mice (Figure 3C).
T12361 967-979 NN denotes Heterozygote
T12362 980-984 NNS denotes pups
T12364 985-989 VBD denotes were
T12363 990-999 VBN denotes recovered
T12365 1000-1004 IN denotes from
T12366 1005-1008 DT denotes the
T12367 1009-1015 NN denotes mating
T12368 1016-1019 DT denotes the
T12369 1020-1028 NNS denotes chimeras
T12370 1029-1033 IN denotes with
T12371 1034-1036 NN denotes WT
T12372 1037-1041 NNS denotes mice
T12373 1042-1043 -LRB- denotes (
T12375 1043-1049 NN denotes Figure
T12374 1050-1052 NN denotes 3C
T12376 1052-1053 -RRB- denotes )
T12377 1053-1054 . denotes .
T12378 1054-1209 sentence denotes These data demonstrate that marker-free RMCE is very efficient in ES cells and allows germline transmission of a targeted mutation (see Figure 1, path A).
T12379 1055-1060 DT denotes These
T12380 1061-1065 NNS denotes data
T12381 1066-1077 VBP denotes demonstrate
T12382 1078-1082 IN denotes that
T12384 1083-1089 NN denotes marker
T12386 1089-1090 HYPH denotes -
T12385 1090-1094 JJ denotes free
T12387 1095-1099 NN denotes RMCE
T12383 1100-1102 VBZ denotes is
T12388 1103-1107 RB denotes very
T12389 1108-1117 JJ denotes efficient
T12390 1118-1120 IN denotes in
T12391 1121-1123 NN denotes ES
T12392 1124-1129 NNS denotes cells
T12393 1130-1133 CC denotes and
T12394 1134-1140 VBZ denotes allows
T12395 1141-1149 NN denotes germline
T12396 1150-1162 NN denotes transmission
T12397 1163-1165 IN denotes of
T12398 1166-1167 DT denotes a
T12400 1168-1176 VBN denotes targeted
T12399 1177-1185 NN denotes mutation
T12401 1186-1187 -LRB- denotes (
T12402 1187-1190 VB denotes see
T12403 1191-1197 NN denotes Figure
T12404 1198-1199 CD denotes 1
T12405 1199-1201 , denotes ,
T12406 1201-1205 NN denotes path
T12407 1206-1207 NN denotes A
T12408 1207-1208 -RRB- denotes )
T12409 1208-1209 . denotes .
R3050 T12167 T12168 det this,possibility
R3056 T12168 T12165 dobj possibility,test
R3060 T12169 T12166 punct ", ",performed
R3064 T12170 T12166 nsubj we,performed
R3067 T12171 T12166 dobj RMCE,performed
R3071 T12172 T12166 prep with,performed
R3076 T12173 T12174 det a,gene
R3078 T12174 T12172 pobj gene,with
R3080 T12175 T12176 compound p53,fusion
R3087 T12176 T12174 compound fusion,gene
R3091 T12177 T12178 prep in,deleted
R3095 T12178 T12174 advcl deleted,gene
R3098 T12179 T12177 pobj which,in
R3102 T12180 T12181 det the,domain
R3104 T12181 T12178 nsubjpass domain,deleted
R3108 T12182 T12181 nmod p53,domain
R3112 T12183 T12184 npadvmod proline,rich
R3116 T12184 T12181 amod rich,domain
R3119 T12185 T12184 punct -,rich
R3123 T12186 T12181 punct (,domain
R3126 T12187 T12181 appos PRD,domain
R3131 T12188 T12178 punct ),deleted
R3137 T12189 T12178 auxpass was,deleted
R3141 T12190 T12191 punct (,p53ΔP
R3145 T12191 T12178 parataxis p53ΔP,deleted
R3149 T12192 T12191 punct ),p53ΔP
R3152 T12193 T12178 punct ", ",deleted
R3156 T12194 T12178 cc and,deleted
R3159 T12195 T12196 det the,p53ΔP
R3160 T12164 T12165 aux To,test
R3161 T12165 T12166 advcl test,performed
R3162 T12196 T12197 nsubjpass p53ΔP,fused
R3163 T12197 T12178 conj fused,deleted
R3164 T12198 T12197 auxpass was,fused
R3165 T12273 T12272 compound ES,cells
R3166 T12274 T12275 punct (,3B
R3167 T12199 T12197 prep to,fused
R3168 T12275 T12257 parataxis 3B,revealed
R3169 T12276 T12275 compound Figure,3B
R3170 T12277 T12275 punct ),3B
R3171 T12200 T12199 pobj GFP,to
R3172 T12278 T12257 punct .,revealed
R3173 T12201 T12166 punct .,performed
R3174 T12280 T12281 mark As,expected
R3175 T12203 T12204 nsubj We,used
R3176 T12281 T12282 advcl expected,correlated
R3177 T12282 T12287 ccomp correlated,was
R3178 T12283 T12282 punct ", ",correlated
R3179 T12205 T12206 det this,mutant
R3180 T12284 T12285 det the,deletion
R3181 T12285 T12282 nsubj deletion,correlated
R3182 T12286 T12285 compound PRD,deletion
R3183 T12206 T12204 dobj mutant,used
R3184 T12288 T12282 prep with,correlated
R3185 T12289 T12290 amod lower,levels
R3186 T12290 T12288 pobj levels,with
R3187 T12291 T12290 compound expression,levels
R3188 T12292 T12287 punct : ,was
R3189 T12207 T12208 mark because,decreases
R3190 T12293 T12287 nsubj p53ΔPGFP,was
R3191 T12294 T12295 advmod much,less
R3192 T12295 T12296 advmod less,abundant
R3193 T12208 T12204 advcl decreases,used
R3194 T12296 T12287 acomp abundant,was
R3195 T12297 T12296 prep than,abundant
R3196 T12298 T12297 pobj p53WT,than
R3197 T12209 T12208 csubj deleting,decreases
R3198 T12299 T12298 prep in,p53WT
R3199 T12300 T12301 det all,clones
R3200 T12301 T12299 pobj clones,in
R3201 T12210 T12211 det the,domain
R3202 T12302 T12303 nmod p53,ΔPGFP
R3203 T12303 T12301 compound ΔPGFP,clones
R3204 T12304 T12303 punct +,ΔPGFP
R3205 T12211 T12209 dobj domain,deleting
R3206 T12305 T12303 punct /,ΔPGFP
R3207 T12306 T12307 punct (,3B
R3208 T12307 T12287 parataxis 3B,was
R3209 T12212 T12213 npadvmod proline,rich
R3210 T12308 T12307 compound Figure,3B
R3211 T12309 T12307 punct ),3B
R3212 T12310 T12287 punct .,was
R3213 T12213 T12211 amod rich,domain
R3214 T12312 T12313 nsubj We,determined
R3215 T12214 T12213 punct -,rich
R3216 T12314 T12313 advmod next,determined
R3217 T12315 T12316 mark whether,generate
R3218 T12215 T12208 dobj stability,decreases
R3219 T12316 T12313 ccomp generate,determined
R3220 T12317 T12318 nmod p53,ΔPGFP
R3221 T12216 T12208 cc and,decreases
R3222 T12318 T12321 compound ΔPGFP,cells
R3223 T12217 T12208 conj compromises,decreases
R3224 T12319 T12318 punct +,ΔPGFP
R3225 T12218 T12219 compound DNA,damage
R3226 T12320 T12318 punct /,ΔPGFP
R3227 T12321 T12316 nsubj cells,generate
R3228 T12219 T12221 compound damage,responses
R3229 T12322 T12321 compound ES,cells
R3230 T12220 T12219 punct -,damage
R3231 T12323 T12316 aux could,generate
R3232 T12324 T12325 amod chimeric,mice
R3233 T12325 T12316 dobj mice,generate
R3234 T12326 T12316 cc and,generate
R3235 T12221 T12217 dobj responses,compromises
R3236 T12327 T12316 conj transmit,generate
R3237 T12328 T12329 det the,allele
R3238 T12329 T12327 dobj allele,transmit
R3239 T12330 T12329 amod modified,allele
R3240 T12331 T12327 prep through,transmit
R3241 T12332 T12333 det the,germline
R3242 T12222 T12223 advmod in,vivo
R3243 T12333 T12331 pobj germline,through
R3244 T12334 T12313 punct .,determined
R3245 T12223 T12217 advmod vivo,compromises
R3246 T12336 T12337 nummod Two,clones
R3247 T12337 T12344 nsubjpass clones,injected
R3248 T12338 T12339 nmod p53,ΔPGFP
R3249 T12224 T12225 punct (,5
R3250 T12339 T12337 compound ΔPGFP,clones
R3251 T12340 T12339 punct +,ΔPGFP
R3252 T12341 T12339 punct /,ΔPGFP
R3253 T12225 T12217 parataxis 5,compromises
R3254 T12342 T12343 compound ES,cell
R3255 T12343 T12337 compound cell,clones
R3256 T12226 T12225 punct ),5
R3257 T12345 T12344 auxpass were,injected
R3258 T12346 T12344 prep into,injected
R3259 T12227 T12204 punct .,used
R3260 T12347 T12346 pobj blastocysts,into
R3261 T12348 T12344 cc and,injected
R3262 T12349 T12350 advmod highly,chimeric
R3263 T12229 T12230 prep According,prevent
R3264 T12350 T12351 amod chimeric,%
R3265 T12351 T12355 amod %,mice
R3266 T12352 T12351 punct (,%
R3267 T12353 T12351 punct >,%
R3268 T12231 T12229 prep to,According
R3269 T12354 T12351 nummod 80,%
R3270 T12355 T12357 nsubjpass mice,obtained
R3271 T12356 T12351 punct ),%
R3272 T12357 T12344 conj obtained,injected
R3273 T12232 T12233 poss our,hypothesis
R3274 T12358 T12357 auxpass were,obtained
R3275 T12359 T12357 punct .,obtained
R3276 T12233 T12231 pobj hypothesis,to
R3277 T12361 T12362 compound Heterozygote,pups
R3278 T12362 T12363 nsubjpass pups,recovered
R3279 T12234 T12230 punct ", ",prevent
R3280 T12364 T12363 auxpass were,recovered
R3281 T12365 T12363 prep from,recovered
R3282 T12235 T12236 det this,mutant
R3283 T12366 T12367 det the,mating
R3284 T12367 T12365 pobj mating,from
R3285 T12236 T12230 nsubj mutant,prevent
R3286 T12237 T12236 amod hypomorphic,mutant
R3287 T12368 T12369 det the,chimeras
R3288 T12369 T12367 nmod chimeras,mating
R3289 T12370 T12367 prep with,mating
R3290 T12371 T12372 compound WT,mice
R3291 T12372 T12370 pobj mice,with
R3292 T12373 T12374 punct (,3C
R3293 T12374 T12363 parataxis 3C,recovered
R3294 T12238 T12230 aux should,prevent
R3295 T12375 T12374 compound Figure,3C
R3296 T12376 T12374 punct ),3C
R3297 T12377 T12363 punct .,recovered
R3298 T12239 T12230 neg not,prevent
R3299 T12240 T12241 amod embryonic,development
R3300 T12241 T12230 dobj development,prevent
R3301 T12242 T12230 punct .,prevent
R3302 T12379 T12380 det These,data
R3303 T12380 T12381 nsubj data,demonstrate
R3304 T12244 T12245 nsubj RMCE,was
R3305 T12382 T12383 mark that,is
R3306 T12383 T12381 ccomp is,demonstrate
R3307 T12384 T12385 npadvmod marker,free
R3308 T12246 T12244 prep with,RMCE
R3309 T12385 T12387 amod free,RMCE
R3310 T12386 T12385 punct -,free
R3311 T12387 T12383 nsubj RMCE,is
R3312 T12388 T12389 advmod very,efficient
R3313 T12247 T12248 det a,plasmid
R3314 T12389 T12383 acomp efficient,is
R3315 T12390 T12383 prep in,is
R3316 T12391 T12392 compound ES,cells
R3317 T12248 T12246 pobj plasmid,with
R3318 T12392 T12390 pobj cells,in
R3319 T12393 T12383 cc and,is
R3320 T12394 T12383 conj allows,is
R3321 T12249 T12248 compound p53ΔPGFP,plasmid
R3322 T12395 T12396 compound germline,transmission
R3323 T12396 T12394 dobj transmission,allows
R3324 T12397 T12396 prep of,transmission
R3325 T12250 T12248 compound replacement,plasmid
R3326 T12398 T12399 det a,mutation
R3327 T12399 T12397 pobj mutation,of
R3328 T12251 T12245 advmod again,was
R3329 T12400 T12399 amod targeted,mutation
R3330 T12401 T12402 punct (,see
R3331 T12402 T12394 parataxis see,allows
R3332 T12252 T12253 advmod very,efficient
R3333 T12403 T12402 dobj Figure,see
R3334 T12404 T12403 nummod 1,Figure
R3335 T12405 T12403 punct ", ",Figure
R3336 T12253 T12245 acomp efficient,was
R3337 T12406 T12407 compound path,A
R3338 T12407 T12403 appos A,Figure
R3339 T12254 T12245 cc and,was
R3340 T12408 T12402 punct ),see
R3341 T12409 T12381 punct .,demonstrate
R3343 T12255 T12256 compound western,blots
R3347 T12256 T12257 nsubj blots,revealed
R3351 T12257 T12245 conj revealed,was
R3355 T12258 T12259 det an,band
R3359 T12259 T12257 dobj band,revealed
R3363 T12260 T12259 amod additional,band
R3367 T12261 T12259 prep of,band
R3371 T12262 T12263 det the,weight
R3375 T12263 T12261 pobj weight,of
R3378 T12264 T12263 amod predicted,weight
R3380 T12265 T12263 amod molecular,weight
R3383 T12266 T12267 advmod only,in
R3384 T12267 T12257 prep in,revealed
R3387 T12268 T12269 nmod p53,ΔPGFP
R3390 T12269 T12272 compound ΔPGFP,cells
R3397 T12270 T12269 punct +,ΔPGFP
R3401 T12271 T12269 punct /,ΔPGFP
R3405 T12272 T12267 pobj cells,in