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PMC:1540739 / 11042-11452 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T6727 0-4 NN denotes Cell
T6728 5-12 NN denotes culture
T6729 13-23 NNS denotes conditions
T6730 23-151 sentence denotes Primary MEFs, isolated from 13.5 day embryos, were cultured in DMEM with 15% FBS, 100 mM BME, 2 mM l-glutamine and antibiotics.
T6731 24-31 JJ denotes Primary
T6732 32-36 NNS denotes MEFs
T6734 36-38 , denotes ,
T6735 38-46 VBN denotes isolated
T6736 47-51 IN denotes from
T6737 52-56 CD denotes 13.5
T6738 57-60 NN denotes day
T6739 61-68 NNS denotes embryos
T6740 68-70 , denotes ,
T6741 70-74 VBD denotes were
T6733 75-83 VBN denotes cultured
T6742 84-86 IN denotes in
T6743 87-91 NN denotes DMEM
T6744 92-96 IN denotes with
T6745 97-99 CD denotes 15
T6746 99-100 NN denotes %
T6747 101-104 NN denotes FBS
T6748 104-106 , denotes ,
T6749 106-109 CD denotes 100
T6750 110-112 NN denotes mM
T6751 113-116 NN denotes BME
T6752 116-118 , denotes ,
T6753 118-119 CD denotes 2
T6754 120-122 NN denotes mM
T6756 123-124 NN denotes l
T6757 124-125 HYPH denotes -
T6755 125-134 NN denotes glutamine
T6758 135-138 CC denotes and
T6759 139-150 NNS denotes antibiotics
T6760 150-151 . denotes .
T6761 151-327 sentence denotes 129/SvJae ES cells were grown in the same medium supplemented with 1000 U/ml ESGRO (Chemicon), on a layer of mitomycin C-treated SNLPuro-7/4 feeders (kind gift of A. Bradley).
T6762 152-155 CD denotes 129
T6764 155-156 HYPH denotes /
T6763 156-161 NN denotes SvJae
T6766 162-164 NN denotes ES
T6765 165-170 NNS denotes cells
T6768 171-175 VBD denotes were
T6767 176-181 VBN denotes grown
T6769 182-184 IN denotes in
T6770 185-188 DT denotes the
T6772 189-193 JJ denotes same
T6771 194-200 NN denotes medium
T6773 201-213 VBN denotes supplemented
T6774 214-218 IN denotes with
T6775 219-223 CD denotes 1000
T6776 224-225 NN denotes U
T6778 225-226 SYM denotes /
T6779 226-228 NN denotes ml
T6777 229-234 NN denotes ESGRO
T6780 235-236 -LRB- denotes (
T6781 236-244 NNP denotes Chemicon
T6782 244-245 -RRB- denotes )
T6783 245-247 , denotes ,
T6784 247-249 IN denotes on
T6785 250-251 DT denotes a
T6786 252-257 NN denotes layer
T6787 258-260 IN denotes of
T6788 261-270 NN denotes mitomycin
T6789 271-272 NN denotes C
T6791 272-273 HYPH denotes -
T6790 273-280 JJ denotes treated
T6793 281-288 NN denotes SNLPuro
T6794 288-289 HYPH denotes -
T6795 289-290 CD denotes 7
T6796 290-291 HYPH denotes /
T6797 291-292 CD denotes 4
T6792 293-300 NNS denotes feeders
T6798 301-302 -LRB- denotes (
T6800 302-306 JJ denotes kind
T6799 307-311 NN denotes gift
T6801 312-314 IN denotes of
T6802 315-317 NNP denotes A.
T6803 318-325 NNP denotes Bradley
T6804 325-326 -RRB- denotes )
T6805 326-327 . denotes .
T6806 327-410 sentence denotes Selections were performed with 2 μg/ml puromycin, 0.2 μM FIAU or 2 μM ganciclovir.
T6807 328-338 NNS denotes Selections
T6809 339-343 VBD denotes were
T6808 344-353 VBN denotes performed
T6810 354-358 IN denotes with
T6811 359-360 CD denotes 2
T6812 361-363 NN denotes μg
T6814 363-364 SYM denotes /
T6815 364-366 NN denotes ml
T6813 367-376 NN denotes puromycin
T6816 376-378 , denotes ,
T6817 378-381 CD denotes 0.2
T6818 382-384 NN denotes μM
T6819 385-389 NN denotes FIAU
T6820 390-392 CC denotes or
T6821 393-394 CD denotes 2
T6822 395-397 NN denotes μM
T6823 398-409 NN denotes ganciclovir
T6824 409-410 . denotes .
R2046 T6727 T6728 compound Cell,culture
R2047 T6728 T6729 compound culture,conditions
R2048 T6731 T6732 amod Primary,MEFs
R2049 T6732 T6733 nsubjpass MEFs,cultured
R2050 T6734 T6732 punct ", ",MEFs
R2051 T6735 T6732 acl isolated,MEFs
R2052 T6736 T6735 prep from,isolated
R2053 T6737 T6738 nummod 13.5,day
R2054 T6738 T6739 compound day,embryos
R2055 T6739 T6736 pobj embryos,from
R2056 T6740 T6733 punct ", ",cultured
R2057 T6741 T6733 auxpass were,cultured
R2058 T6742 T6733 prep in,cultured
R2059 T6743 T6742 pobj DMEM,in
R2060 T6744 T6733 prep with,cultured
R2061 T6745 T6746 nummod 15,%
R2062 T6746 T6747 compound %,FBS
R2063 T6747 T6744 pobj FBS,with
R2064 T6748 T6747 punct ", ",FBS
R2065 T6749 T6750 nummod 100,mM
R2066 T6750 T6751 compound mM,BME
R2067 T6751 T6747 conj BME,FBS
R2068 T6752 T6751 punct ", ",BME
R2069 T6753 T6754 nummod 2,mM
R2070 T6754 T6755 compound mM,glutamine
R2071 T6755 T6751 conj glutamine,BME
R2072 T6756 T6755 compound l,glutamine
R2073 T6757 T6755 punct -,glutamine
R2074 T6758 T6755 cc and,glutamine
R2075 T6759 T6755 conj antibiotics,glutamine
R2076 T6760 T6733 punct .,cultured
R2077 T6762 T6763 nummod 129,SvJae
R2078 T6763 T6765 compound SvJae,cells
R2079 T6764 T6763 punct /,SvJae
R2080 T6765 T6767 nsubjpass cells,grown
R2081 T6766 T6765 compound ES,cells
R2082 T6768 T6767 auxpass were,grown
R2083 T6769 T6767 prep in,grown
R2084 T6770 T6771 det the,medium
R2085 T6771 T6769 pobj medium,in
R2086 T6772 T6771 amod same,medium
R2087 T6773 T6771 acl supplemented,medium
R2088 T6774 T6773 prep with,supplemented
R2089 T6775 T6776 nummod 1000,U
R2090 T6776 T6777 nmod U,ESGRO
R2091 T6777 T6774 pobj ESGRO,with
R2092 T6778 T6779 punct /,ml
R2093 T6779 T6776 prep ml,U
R2094 T6780 T6781 punct (,Chemicon
R2095 T6781 T6777 parataxis Chemicon,ESGRO
R2096 T6782 T6781 punct ),Chemicon
R2097 T6783 T6767 punct ", ",grown
R2098 T6784 T6767 prep on,grown
R2099 T6785 T6786 det a,layer
R2100 T6786 T6784 pobj layer,on
R2101 T6787 T6786 prep of,layer
R2102 T6788 T6789 compound mitomycin,C
R2103 T6789 T6790 npadvmod C,treated
R2104 T6790 T6792 amod treated,feeders
R2105 T6791 T6790 punct -,treated
R2106 T6792 T6787 pobj feeders,of
R2107 T6793 T6792 nmod SNLPuro,feeders
R2108 T6794 T6793 punct -,SNLPuro
R2109 T6795 T6793 nummod 7,SNLPuro
R2110 T6796 T6793 punct /,SNLPuro
R2111 T6797 T6793 nummod 4,SNLPuro
R2112 T6798 T6799 punct (,gift
R2113 T6799 T6786 parataxis gift,layer
R2114 T6800 T6799 amod kind,gift
R2115 T6801 T6799 prep of,gift
R2116 T6802 T6803 compound A.,Bradley
R2117 T6803 T6801 pobj Bradley,of
R2118 T6804 T6799 punct ),gift
R2119 T6805 T6767 punct .,grown
R2120 T6807 T6808 nsubjpass Selections,performed
R2121 T6809 T6808 auxpass were,performed
R2122 T6810 T6808 prep with,performed
R2123 T6811 T6812 nummod 2,μg
R2124 T6812 T6813 nmod μg,puromycin
R2125 T6813 T6810 pobj puromycin,with
R2126 T6814 T6815 punct /,ml
R2127 T6815 T6812 prep ml,μg
R2128 T6816 T6813 punct ", ",puromycin
R2129 T6817 T6818 nummod 0.2,μM
R2130 T6818 T6819 compound μM,FIAU
R2131 T6819 T6813 conj FIAU,puromycin
R2132 T6820 T6819 cc or,FIAU
R2133 T6821 T6822 nummod 2,μM
R2134 T6822 T6823 compound μM,ganciclovir
R2135 T6823 T6819 conj ganciclovir,FIAU
R2136 T6824 T6808 punct .,performed