PMC:149366 / 11-9929 JSON TXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T191	0-6	JJ	denotes	mutant
T1848	0-5567	NNS	denotes	mutant mice show long-term, incomplete retention of some afferent inner ear innervation Abstract Background Ears of Brn3c null mutants develop immature hair cells, identifiable only by certain molecular markers, and undergo apoptosis in neonates. This partial development of hair cells could lead to enough neurotrophin expression to sustain sensory neurons through embryonic development. We have therefore investigated in these mutants the patterns of innervation and of expression of known neurotrophins. Results At birth there is a limited expression of BDNF and NT-3 in the mutant sensory epithelia and DiI tracing shows no specific reduction of afferents or efferents that resembles neurotrophin null mutations. At postnatal day 7/8 (P7/8), innervation is severely reduced both qualitatively and quantitatively. 1% of myosin VIIa-positive immature hair cells are present in the mutant cochlea, concentrated in the base. Around 20% of immature hair cells exist in the mutant vestibular sensory epithelia. Despite more severe loss of hair cells (1% compared to 20%), the cochlea retains many more sensory neurons (46% compared to 15%) than vestibular epithelia. Even 6 months old mutant mice have some fibers to all vestibular sensory epithelia and many more to the cochlear apex which lacks MyoVIIa positive hair cells. Topologically organized central cochlea projections exist at least until P8, suggesting that functional hair cells are not required to establish such projections. Conclusion The limited expression of neurotrophins in the cochlea of Brn3c null mice suffices to support many sensory neurons, particularly in the cochlea, until birth. The molecular nature of the long term survival of apical spiral neurons remains unclear. Background Brn3c is a POU domain factor that is crucial for inner ear hair cell development. Targeted null Brn3c mutants have no mature hair cells [1,2]. Close examination has revealed that some 'immature' hair cells form in Brn3c null mutants and express cellular markers such as Myosin VI and VIIa, calretinin and parvalbumin [3]. Furthermore, these immature hair cells of Brn3c null mutants undergo apoptosis in neonates [3]. Consistent with an apparent absence of mature hair cells, initial work suggested that all vestibular and most spiral ganglion cells are lost by postnatal day 14 (P14; [1]). However, more detailed quantification by others [3] reported that at P4 about 77% of vestibular neurons and only 29% of spiral neurons are lost. It was suggested that there is possibly a complete loss in adults [3]. Other than these preliminary statements, no data exists concerning the detailed pattern of loss of innervation in Brn3c null mutants. The initial development and partial differentiation of hair cells in Brn3c mutants could possibly lead to some neurotrophin expression in these cells to sustain sensory neurons through embryonic development and beyond. Data on various single and compound neurotrophin null mutants have shown that the loss of a specific neurotrophin leads to topologically restricted loss of sensory neurons in the embryonic ear [4,5]. Such selective loss in Brn3c null mutants would therefore indicate reduction of a specific neurotrophin in immature hair cells. Moreover, recent work shows that in embryos NT-3 is primarily expressed in supporting cells, moving only around birth into hair cells [6,7]. In fact, the selective loss of vestibular as compared to cochlear sensory neurons (77% versus 29%; [3]) suggests that NT-3 expression may be less downregulated in Brn3c null mutants than BDNF [6,8,9], provided that at least some differentiation of supporting cells takes place. In the ear [4] as well as elsewhere [10] neurotrophins are progressively downregulated in postnatal mammals and possibly replaced by other factors [11]. We have investigated in detail the pattern of innervation in the Brn3c mutants, as well as the expression of NT-3 and BDNF. We want to evaluate a possible correlation between the topology of sensory neuron loss and absence of a specific neurotrophin or topological loss of hair cells at birth and in older animals. This information could be important for an in-depth evaluation of the human deafness related to the Pou4f3 gene, DFNA15 [12]. We report here long term retention of cochlear sensory neurons for at least 6 months, in particular in the cochlear apex, in Brn3c null mutant mice. This retention of afferents and efferents is unrelated to hair cell differentiation as not even immature hair cells can be detected at early postnatal stages with MyoVII immunocytochemistry in this part of the cochlea. This retention of apical spiral neurons is also largely unrelated to neurotrophins which are known to be reduced in their expression in neonatal rodents [4]. Results To appreciate the effects of the Brn3c null mutation on the pattern of the inner ear innervation, we first want to present the effects of BDNF and NT-3 null mutations at birth [6,13,14]. Null mutants of BDNF or its receptor trkB lose all innervation to the semicircular canals and have a reduced innervation to the utricle, saccule and apical turn of the cochlea. In contrast, null mutations of either NT-3 or its receptor trkC result in loss of spiral neurons in the basal turn with formation of an inner spiral bundle of afferents extending to the basal tip. Our null hypothesis for this study would be that Brn3c null mice show severe compromised production of these neurotrophins and should therefore show a comparable pattern of nerve fiber loss. Brn3c null mutants
T2768	0-9918	NNS	denotes	mutant mice show long-term, incomplete retention of some afferent inner ear innervation Abstract Background Ears of Brn3c null mutants develop immature hair cells, identifiable only by certain molecular markers, and undergo apoptosis in neonates. This partial development of hair cells could lead to enough neurotrophin expression to sustain sensory neurons through embryonic development. We have therefore investigated in these mutants the patterns of innervation and of expression of known neurotrophins. Results At birth there is a limited expression of BDNF and NT-3 in the mutant sensory epithelia and DiI tracing shows no specific reduction of afferents or efferents that resembles neurotrophin null mutations. At postnatal day 7/8 (P7/8), innervation is severely reduced both qualitatively and quantitatively. 1% of myosin VIIa-positive immature hair cells are present in the mutant cochlea, concentrated in the base. Around 20% of immature hair cells exist in the mutant vestibular sensory epithelia. Despite more severe loss of hair cells (1% compared to 20%), the cochlea retains many more sensory neurons (46% compared to 15%) than vestibular epithelia. Even 6 months old mutant mice have some fibers to all vestibular sensory epithelia and many more to the cochlear apex which lacks MyoVIIa positive hair cells. Topologically organized central cochlea projections exist at least until P8, suggesting that functional hair cells are not required to establish such projections. Conclusion The limited expression of neurotrophins in the cochlea of Brn3c null mice suffices to support many sensory neurons, particularly in the cochlea, until birth. The molecular nature of the long term survival of apical spiral neurons remains unclear. Background Brn3c is a POU domain factor that is crucial for inner ear hair cell development. Targeted null Brn3c mutants have no mature hair cells [1,2]. Close examination has revealed that some 'immature' hair cells form in Brn3c null mutants and express cellular markers such as Myosin VI and VIIa, calretinin and parvalbumin [3]. Furthermore, these immature hair cells of Brn3c null mutants undergo apoptosis in neonates [3]. Consistent with an apparent absence of mature hair cells, initial work suggested that all vestibular and most spiral ganglion cells are lost by postnatal day 14 (P14; [1]). However, more detailed quantification by others [3] reported that at P4 about 77% of vestibular neurons and only 29% of spiral neurons are lost. It was suggested that there is possibly a complete loss in adults [3]. Other than these preliminary statements, no data exists concerning the detailed pattern of loss of innervation in Brn3c null mutants. The initial development and partial differentiation of hair cells in Brn3c mutants could possibly lead to some neurotrophin expression in these cells to sustain sensory neurons through embryonic development and beyond. Data on various single and compound neurotrophin null mutants have shown that the loss of a specific neurotrophin leads to topologically restricted loss of sensory neurons in the embryonic ear [4,5]. Such selective loss in Brn3c null mutants would therefore indicate reduction of a specific neurotrophin in immature hair cells. Moreover, recent work shows that in embryos NT-3 is primarily expressed in supporting cells, moving only around birth into hair cells [6,7]. In fact, the selective loss of vestibular as compared to cochlear sensory neurons (77% versus 29%; [3]) suggests that NT-3 expression may be less downregulated in Brn3c null mutants than BDNF [6,8,9], provided that at least some differentiation of supporting cells takes place. In the ear [4] as well as elsewhere [10] neurotrophins are progressively downregulated in postnatal mammals and possibly replaced by other factors [11]. We have investigated in detail the pattern of innervation in the Brn3c mutants, as well as the expression of NT-3 and BDNF. We want to evaluate a possible correlation between the topology of sensory neuron loss and absence of a specific neurotrophin or topological loss of hair cells at birth and in older animals. This information could be important for an in-depth evaluation of the human deafness related to the Pou4f3 gene, DFNA15 [12]. We report here long term retention of cochlear sensory neurons for at least 6 months, in particular in the cochlear apex, in Brn3c null mutant mice. This retention of afferents and efferents is unrelated to hair cell differentiation as not even immature hair cells can be detected at early postnatal stages with MyoVII immunocytochemistry in this part of the cochlea. This retention of apical spiral neurons is also largely unrelated to neurotrophins which are known to be reduced in their expression in neonatal rodents [4]. Results To appreciate the effects of the Brn3c null mutation on the pattern of the inner ear innervation, we first want to present the effects of BDNF and NT-3 null mutations at birth [6,13,14]. Null mutants of BDNF or its receptor trkB lose all innervation to the semicircular canals and have a reduced innervation to the utricle, saccule and apical turn of the cochlea. In contrast, null mutations of either NT-3 or its receptor trkC result in loss of spiral neurons in the basal turn with formation of an inner spiral bundle of afferents extending to the basal tip. Our null hypothesis for this study would be that Brn3c null mice show severe compromised production of these neurotrophins and should therefore show a comparable pattern of nerve fiber loss. Brn3c null mutants at birth (P0) Vestibular ganglia are smaller in Brn3c null mutants (Fig. 1b) than in control littermates (Fig. 1a), but larger than in BDNF or trkB null mutants of the same age [13,15]. The reduction in apparent size of the vestibular ganglia is in agreement with quantitative data published previously [3,13]. These data suggest a loss of 80–85% of vestibular sensory neurons in BDNF and trkB null mutants [13] and of 77% of vestibular sensory neurons in P4 Brn3c null mutants [3]. Thus, the size reduction in the vestibular sensory ganglion could be compatible with a loss of BDNF production in the immature hair cells. Figure 1 Size variations of vestibular ganglia in control and mutant littermates labeled with DiI. In newborn animals, the vestibular ganglion shows a dramatic reduction in Brn3c null mutants (b) compared to control littermates (a). Abbreviations for this and other figures: AC, anterior crista; ggl, ganglion; c, spiral capillary; Genic. ggl., geniculate ganglion; GER, greater epithelial ridge; HC, horizontal crista; HaC, vestibular hair cell; IHC, inner hair cell; iHC, immature hair cell; IGSB, intraganglionic spiral bundle; OHC, outer hair cell; PC, posterior crista; S, saccule; SG, spiral ganglion; TM, tectorial membrane; U, utricle; VCN, ventral cochlear nucleus; VG, vestibular ganglion. Bar indicates 1000 μm. However, the Brn3c null mutants show only a reduced density of afferent and efferent fibers to all vestibular sensory epithelia. There is no specific loss of all afferent and efferent innervation to the canal crista (Fig. 2a), a hallmark of both BDNF and trkB null mutations [13,15]. In fact, the reduction of fibers seems to be rather uniform throughout a given sensory epithelium with the crista innervation being qualitatively no more reduced than the innervation of the utricle and saccule. No loss in specific areas, comparable to that in BDNF null mutant mice, is apparent in the saccule or utricle of Brn3c null mice. Similar patterns of innervation are obtained using acteylated tubulin immunocytochemistry (Fig. 2b). Figure 2 Innervation of Brn3c null and control ears are shown for newborn mice. There is no specific loss of fibers to any vestibular endorgan, as visualized by DiI labeling (a) or acetylated tubulin immunoreactivity (b). No major differences in pattern of projection through radial fibers are found in the cochlea of Brn3c null mutants (d,f) as compared to control littermates (c). Note, however, the lack of orderly fiber outgrowth to the outer hair cells (c, d). Efferent fibers to the ear show a well developed intraganglionic spiral bundle (IGSB) with no detectable differences compared to controls (e). Bar indicates 100 μm. Consistent with the finding of Xiang et al. [3] of only a 29% loss of spiral sensory neurons at P4, our data show little difference in the pattern of innervation of the cochlea in P0 Brn3c null mutants (Fig. 2c,2d). No selective loss of spiral neurons is observed in Brn3c null mutants in the basal turn, a feature of either NT-3 or trkC loss [6,9,14]. Likewise, the innervation of the apex (Fig. 2f) shows no detectable abnormality in overall pattern of innervation compared to control animals (data not shown), an indication that BDNF could be expressed in the apex [6]. In addition, the pattern of efferent innervation shows no deviation from normal either (Fig. 2e), whereas they show the same pattern of loss as afferent fibers in neurotrophin null mutants [16]. These data suggest that the spiral sensory neurons develop qualitatively normal at least until P0 and therefore allow normal pathfinding of efferents. Most interestingly, there is no increase in radial fiber spacing in the apex, a specific problem of BDNF null mutants [6,13]. However, there is one qualitative difference not recognized in any single neurotrophin null mutant. Afferents reach all three rows of outer hair cells in the basal turn of control wildtype littermates (Fig. 2c), but both afferent and efferent outgrowth is disorganized to outer hair cells in Brn3c null mutants (Fig. 2d) and does not show any clear organization into three distinct longitudinal fiber bundles paralleling the three rows in the outer hair cell region. These data suggest that fiber organization in the outer hair cell region is partly disrupted in Brn3c null mutant. Brn3c null mutants
T190	7-11	NNS	denotes	mice
T192	12-16	VBP	denotes	show
T193	17-21	JJ	denotes	long
T195	21-22	HYPH	denotes	-
T194	22-26	NN	denotes	term
T197	26-28	,	denotes	,
T198	28-38	JJ	denotes	incomplete
T196	39-48	NN	denotes	retention
T199	49-51	IN	denotes	of
T200	52-56	DT	denotes	some
T202	57-65	JJ	denotes	afferent
T203	66-71	JJ	denotes	inner
T204	72-75	NN	denotes	ear
T201	76-87	NN	denotes	innervation
T205	87-88	sentence	denotes
T206	98-108	sentence	denotes	Background
T207	98-108	NN	denotes	Background
T208	108-247	sentence	denotes	Ears of Brn3c null mutants develop immature hair cells, identifiable only by certain molecular markers, and undergo apoptosis in neonates.
T209	109-113	NNS	denotes	Ears
T211	114-116	IN	denotes	of
T212	117-122	NN	denotes	Brn3c
T214	123-127	JJ	denotes	null
T213	128-135	NNS	denotes	mutants
T210	136-143	VBP	denotes	develop
T215	144-152	JJ	denotes	immature
T217	153-157	NN	denotes	hair
T216	158-163	NNS	denotes	cells
T218	163-165	,	denotes	,
T219	165-177	JJ	denotes	identifiable
T220	178-182	RB	denotes	only
T221	183-185	IN	denotes	by
T222	186-193	JJ	denotes	certain
T224	194-203	JJ	denotes	molecular
T223	204-211	NNS	denotes	markers
T225	211-213	,	denotes	,
T226	213-216	CC	denotes	and
T227	217-224	VBP	denotes	undergo
T228	225-234	NN	denotes	apoptosis
T229	235-237	IN	denotes	in
T230	238-246	NNS	denotes	neonates
T231	246-247	.	denotes	.
T232	247-389	sentence	denotes	This partial development of hair cells could lead to enough neurotrophin expression to sustain sensory neurons through embryonic development.
T233	248-252	DT	denotes	This
T235	253-260	JJ	denotes	partial
T234	261-272	NN	denotes	development
T237	273-275	IN	denotes	of
T238	276-280	NN	denotes	hair
T239	281-286	NNS	denotes	cells
T240	287-292	MD	denotes	could
T236	293-297	VB	denotes	lead
T241	298-300	IN	denotes	to
T242	301-307	JJ	denotes	enough
T244	308-320	NN	denotes	neurotrophin
T243	321-331	NN	denotes	expression
T245	332-334	TO	denotes	to
T246	335-342	VB	denotes	sustain
T247	343-350	JJ	denotes	sensory
T248	351-358	NNS	denotes	neurons
T249	359-366	IN	denotes	through
T250	367-376	JJ	denotes	embryonic
T251	377-388	NN	denotes	development
T252	388-389	.	denotes	.
T253	389-507	sentence	denotes	We have therefore investigated in these mutants the patterns of innervation and of expression of known neurotrophins.
T254	390-392	PRP	denotes	We
T256	393-397	VBP	denotes	have
T257	398-407	RB	denotes	therefore
T255	408-420	VBN	denotes	investigated
T258	421-423	IN	denotes	in
T259	424-429	DT	denotes	these
T260	430-437	NNS	denotes	mutants
T261	438-441	DT	denotes	the
T262	442-450	NNS	denotes	patterns
T263	451-453	IN	denotes	of
T264	454-465	NN	denotes	innervation
T265	466-469	CC	denotes	and
T266	470-472	IN	denotes	of
T267	473-483	NN	denotes	expression
T268	484-486	IN	denotes	of
T269	487-492	JJ	denotes	known
T270	493-506	NNS	denotes	neurotrophins
T271	506-507	.	denotes	.
T272	507-516	sentence	denotes	Results
T273	509-516	NNS	denotes	Results
T274	516-718	sentence	denotes	At birth there is a limited expression of BDNF and NT-3 in the mutant sensory epithelia and DiI tracing shows no specific reduction of afferents or efferents that resembles neurotrophin null mutations.
T275	517-519	IN	denotes	At
T277	520-525	NN	denotes	birth
T278	526-531	EX	denotes	there
T276	532-534	VBZ	denotes	is
T279	535-536	DT	denotes	a
T281	537-544	VBN	denotes	limited
T280	545-555	NN	denotes	expression
T282	556-558	IN	denotes	of
T283	559-563	NN	denotes	BDNF
T284	564-567	CC	denotes	and
T285	568-570	NN	denotes	NT
T286	570-571	HYPH	denotes	-
T287	571-572	CD	denotes	3
T288	573-575	IN	denotes	in
T289	576-579	DT	denotes	the
T291	580-586	JJ	denotes	mutant
T292	587-594	JJ	denotes	sensory
T290	595-604	NNS	denotes	epithelia
T293	605-608	CC	denotes	and
T294	609-612	NN	denotes	DiI
T295	613-620	NN	denotes	tracing
T296	621-626	VBZ	denotes	shows
T297	627-629	DT	denotes	no
T299	630-638	JJ	denotes	specific
T298	639-648	NN	denotes	reduction
T300	649-651	IN	denotes	of
T301	652-661	NNS	denotes	afferents
T302	662-664	CC	denotes	or
T303	665-674	NNS	denotes	efferents
T304	675-679	WDT	denotes	that
T305	680-689	VBZ	denotes	resembles
T306	690-702	NN	denotes	neurotrophin
T308	703-707	JJ	denotes	null
T307	708-717	NNS	denotes	mutations
T309	717-718	.	denotes	.
T310	718-818	sentence	denotes	At postnatal day 7/8 (P7/8), innervation is severely reduced both qualitatively and quantitatively.
T311	719-721	IN	denotes	At
T313	722-731	JJ	denotes	postnatal
T314	732-735	NN	denotes	day
T315	736-737	CD	denotes	7
T316	737-738	HYPH	denotes	/
T317	738-739	CD	denotes	8
T318	740-741	-LRB-	denotes	(
T319	741-743	NN	denotes	P7
T320	743-744	HYPH	denotes	/
T321	744-745	CD	denotes	8
T322	745-746	-RRB-	denotes	)
T323	746-748	,	denotes	,
T324	748-759	NN	denotes	innervation
T325	760-762	VBZ	denotes	is
T326	763-771	RB	denotes	severely
T312	772-779	VBN	denotes	reduced
T327	780-784	CC	denotes	both
T328	785-798	RB	denotes	qualitatively
T329	799-802	CC	denotes	and
T330	803-817	RB	denotes	quantitatively
T331	817-818	.	denotes	.
T332	818-926	sentence	denotes	1% of myosin VIIa-positive immature hair cells are present in the mutant cochlea, concentrated in the base.
T333	819-820	CD	denotes	1
T334	820-821	NN	denotes	%
T336	822-824	IN	denotes	of
T337	825-831	NN	denotes	myosin
T338	832-836	NN	denotes	VIIa
T340	836-837	HYPH	denotes	-
T339	837-845	JJ	denotes	positive
T342	846-854	JJ	denotes	immature
T343	855-859	NN	denotes	hair
T341	860-865	NNS	denotes	cells
T335	866-869	VBP	denotes	are
T344	870-877	JJ	denotes	present
T345	878-880	IN	denotes	in
T346	881-884	DT	denotes	the
T348	885-891	JJ	denotes	mutant
T347	892-899	NN	denotes	cochlea
T349	899-901	,	denotes	,
T350	901-913	VBD	denotes	concentrated
T351	914-916	IN	denotes	in
T352	917-920	DT	denotes	the
T353	921-925	NN	denotes	base
T354	925-926	.	denotes	.
T355	926-1010	sentence	denotes	Around 20% of immature hair cells exist in the mutant vestibular sensory epithelia.
T356	927-933	IN	denotes	Around
T357	934-936	CD	denotes	20
T358	936-937	NN	denotes	%
T360	938-940	IN	denotes	of
T361	941-949	JJ	denotes	immature
T363	950-954	NN	denotes	hair
T362	955-960	NNS	denotes	cells
T359	961-966	VBP	denotes	exist
T364	967-969	IN	denotes	in
T365	970-973	DT	denotes	the
T367	974-980	JJ	denotes	mutant
T368	981-991	JJ	denotes	vestibular
T369	992-999	JJ	denotes	sensory
T366	1000-1009	NNS	denotes	epithelia
T370	1009-1010	.	denotes	.
T371	1010-1166	sentence	denotes	Despite more severe loss of hair cells (1% compared to 20%), the cochlea retains many more sensory neurons (46% compared to 15%) than vestibular epithelia.
T372	1011-1018	IN	denotes	Despite
T374	1019-1023	RBR	denotes	more
T375	1024-1030	JJ	denotes	severe
T376	1031-1035	NN	denotes	loss
T377	1036-1038	IN	denotes	of
T378	1039-1043	NN	denotes	hair
T379	1044-1049	NNS	denotes	cells
T380	1050-1051	-LRB-	denotes	(
T382	1051-1052	CD	denotes	1
T381	1052-1053	NN	denotes	%
T383	1054-1062	VBN	denotes	compared
T384	1063-1065	IN	denotes	to
T385	1066-1068	CD	denotes	20
T386	1068-1069	NN	denotes	%
T387	1069-1070	-RRB-	denotes	)
T388	1070-1072	,	denotes	,
T389	1072-1075	DT	denotes	the
T390	1076-1083	NN	denotes	cochlea
T373	1084-1091	VBZ	denotes	retains
T391	1092-1096	JJ	denotes	many
T393	1097-1101	JJR	denotes	more
T394	1102-1109	JJ	denotes	sensory
T392	1110-1117	NNS	denotes	neurons
T395	1118-1119	-LRB-	denotes	(
T397	1119-1121	CD	denotes	46
T396	1121-1122	NN	denotes	%
T398	1123-1131	VBN	denotes	compared
T399	1132-1134	IN	denotes	to
T400	1135-1137	CD	denotes	15
T401	1137-1138	NN	denotes	%
T402	1138-1139	-RRB-	denotes	)
T403	1140-1144	IN	denotes	than
T404	1145-1155	JJ	denotes	vestibular
T405	1156-1165	NNS	denotes	epithelia
T406	1165-1166	.	denotes	.
T407	1166-1325	sentence	denotes	Even 6 months old mutant mice have some fibers to all vestibular sensory epithelia and many more to the cochlear apex which lacks MyoVIIa positive hair cells.
T408	1167-1171	RB	denotes	Even
T410	1172-1173	CD	denotes	6
T411	1174-1180	NNS	denotes	months
T412	1181-1184	JJ	denotes	old
T413	1185-1191	JJ	denotes	mutant
T409	1192-1196	NNS	denotes	mice
T414	1197-1201	VBP	denotes	have
T415	1202-1206	DT	denotes	some
T416	1207-1213	NNS	denotes	fibers
T417	1214-1216	IN	denotes	to
T418	1217-1220	DT	denotes	all
T420	1221-1231	JJ	denotes	vestibular
T421	1232-1239	JJ	denotes	sensory
T419	1240-1249	NNS	denotes	epithelia
T422	1250-1253	CC	denotes	and
T423	1254-1258	JJ	denotes	many
T424	1259-1263	JJR	denotes	more
T425	1264-1266	IN	denotes	to
T426	1267-1270	DT	denotes	the
T428	1271-1279	JJ	denotes	cochlear
T427	1280-1284	NN	denotes	apex
T429	1285-1290	WDT	denotes	which
T430	1291-1296	VBZ	denotes	lacks
T431	1297-1304	NN	denotes	MyoVIIa
T432	1305-1313	JJ	denotes	positive
T434	1314-1318	NN	denotes	hair
T433	1319-1324	NNS	denotes	cells
T435	1324-1325	.	denotes	.
T436	1325-1488	sentence	denotes	Topologically organized central cochlea projections exist at least until P8, suggesting that functional hair cells are not required to establish such projections.
T437	1326-1339	RB	denotes	Topologically
T438	1340-1349	VBN	denotes	organized
T440	1350-1357	JJ	denotes	central
T441	1358-1365	NN	denotes	cochlea
T439	1366-1377	NNS	denotes	projections
T442	1378-1383	VBP	denotes	exist
T443	1384-1386	RB	denotes	at
T444	1387-1392	RBS	denotes	least
T445	1393-1398	IN	denotes	until
T446	1399-1401	NN	denotes	P8
T447	1401-1403	,	denotes	,
T448	1403-1413	VBG	denotes	suggesting
T449	1414-1418	IN	denotes	that
T451	1419-1429	JJ	denotes	functional
T453	1430-1434	NN	denotes	hair
T452	1435-1440	NNS	denotes	cells
T454	1441-1444	VBP	denotes	are
T455	1445-1448	RB	denotes	not
T450	1449-1457	VBN	denotes	required
T456	1458-1460	TO	denotes	to
T457	1461-1470	VB	denotes	establish
T458	1471-1475	JJ	denotes	such
T459	1476-1487	NNS	denotes	projections
T460	1487-1488	.	denotes	.
T461	1488-1500	sentence	denotes	Conclusion
T462	1490-1500	NN	denotes	Conclusion
T463	1500-1658	sentence	denotes	The limited expression of neurotrophins in the cochlea of Brn3c null mice suffices to support many sensory neurons, particularly in the cochlea, until birth.
T464	1501-1504	DT	denotes	The
T466	1505-1512	VBN	denotes	limited
T465	1513-1523	NN	denotes	expression
T468	1524-1526	IN	denotes	of
T469	1527-1540	NNS	denotes	neurotrophins
T470	1541-1543	IN	denotes	in
T471	1544-1547	DT	denotes	the
T472	1548-1555	NN	denotes	cochlea
T473	1556-1558	IN	denotes	of
T474	1559-1564	NN	denotes	Brn3c
T475	1565-1569	JJ	denotes	null
T476	1570-1574	NNS	denotes	mice
T467	1575-1583	VBZ	denotes	suffices
T477	1584-1586	TO	denotes	to
T478	1587-1594	VB	denotes	support
T479	1595-1599	JJ	denotes	many
T481	1600-1607	JJ	denotes	sensory
T480	1608-1615	NNS	denotes	neurons
T482	1615-1617	,	denotes	,
T483	1617-1629	RB	denotes	particularly
T484	1630-1632	IN	denotes	in
T485	1633-1636	DT	denotes	the
T486	1637-1644	NN	denotes	cochlea
T487	1644-1646	,	denotes	,
T488	1646-1651	IN	denotes	until
T489	1652-1657	NN	denotes	birth
T490	1657-1658	.	denotes	.
T491	1658-1747	sentence	denotes	The molecular nature of the long term survival of apical spiral neurons remains unclear.
T492	1659-1662	DT	denotes	The
T494	1663-1672	JJ	denotes	molecular
T493	1673-1679	NN	denotes	nature
T496	1680-1682	IN	denotes	of
T497	1683-1686	DT	denotes	the
T499	1687-1691	JJ	denotes	long
T500	1692-1696	NN	denotes	term
T498	1697-1705	NN	denotes	survival
T501	1706-1708	IN	denotes	of
T502	1709-1715	JJ	denotes	apical
T503	1716-1722	JJ	denotes	spiral
T504	1723-1730	NNS	denotes	neurons
T495	1731-1738	VBZ	denotes	remains
T505	1739-1746	JJ	denotes	unclear
T506	1746-1747	.	denotes	.
T820	1760-1765	NN	denotes	Brn3c
T821	1766-1768	VBZ	denotes	is
T822	1769-1770	DT	denotes	a
T824	1771-1774	NN	denotes	POU
T825	1775-1781	NN	denotes	domain
T823	1782-1788	NN	denotes	factor
T826	1789-1793	WDT	denotes	that
T827	1794-1796	VBZ	denotes	is
T828	1797-1804	JJ	denotes	crucial
T829	1805-1808	IN	denotes	for
T830	1809-1814	JJ	denotes	inner
T831	1815-1818	JJ	denotes	ear
T833	1819-1823	NN	denotes	hair
T834	1824-1828	NN	denotes	cell
T832	1829-1840	NN	denotes	development
T835	1840-1841	.	denotes	.
T836	1841-1902	sentence	denotes	Targeted null Brn3c mutants have no mature hair cells [1,2].
T837	1842-1850	VBN	denotes	Targeted
T839	1851-1855	JJ	denotes	null
T840	1856-1861	NN	denotes	Brn3c
T838	1862-1869	NNS	denotes	mutants
T841	1870-1874	VBP	denotes	have
T842	1875-1877	DT	denotes	no
T844	1878-1884	JJ	denotes	mature
T845	1885-1889	NN	denotes	hair
T843	1890-1895	NNS	denotes	cells
T846	1896-1897	-LRB-	denotes	[
T848	1897-1898	CD	denotes	1
T849	1898-1899	,	denotes	,
T847	1899-1900	CD	denotes	2
T850	1900-1901	-RRB-	denotes	]
T851	1901-1902	.	denotes	.
T852	1902-2081	sentence	denotes	Close examination has revealed that some 'immature' hair cells form in Brn3c null mutants and express cellular markers such as Myosin VI and VIIa, calretinin and parvalbumin [3].
T853	1903-1908	NN	denotes	Close
T854	1909-1920	NN	denotes	examination
T856	1921-1924	VBZ	denotes	has
T855	1925-1933	VBN	denotes	revealed
T857	1934-1938	IN	denotes	that
T859	1939-1943	DT	denotes	some
T861	1944-1945	``	denotes	'
T862	1945-1953	JJ	denotes	immature
T863	1953-1954	''	denotes	'
T864	1955-1959	NN	denotes	hair
T860	1960-1965	NNS	denotes	cells
T858	1966-1970	VBP	denotes	form
T865	1971-1973	IN	denotes	in
T866	1974-1979	NN	denotes	Brn3c
T868	1980-1984	JJ	denotes	null
T867	1985-1992	NNS	denotes	mutants
T869	1993-1996	CC	denotes	and
T870	1997-2004	VBP	denotes	express
T871	2005-2013	JJ	denotes	cellular
T872	2014-2021	NNS	denotes	markers
T873	2022-2026	JJ	denotes	such
T874	2027-2029	IN	denotes	as
T875	2030-2036	NN	denotes	Myosin
T876	2037-2039	NN	denotes	VI
T877	2040-2043	CC	denotes	and
T878	2044-2048	NN	denotes	VIIa
T879	2048-2050	,	denotes	,
T880	2050-2060	NN	denotes	calretinin
T881	2061-2064	CC	denotes	and
T882	2065-2076	NN	denotes	parvalbumin
T883	2077-2078	-LRB-	denotes	[
T884	2078-2079	CD	denotes	3
T885	2079-2080	-RRB-	denotes	]
T886	2080-2081	.	denotes	.
T887	2081-2177	sentence	denotes	Furthermore, these immature hair cells of Brn3c null mutants undergo apoptosis in neonates [3].
T888	2082-2093	RB	denotes	Furthermore
T890	2093-2095	,	denotes	,
T891	2095-2100	DT	denotes	these
T893	2101-2109	JJ	denotes	immature
T894	2110-2114	NN	denotes	hair
T892	2115-2120	NNS	denotes	cells
T895	2121-2123	IN	denotes	of
T896	2124-2129	NN	denotes	Brn3c
T898	2130-2134	JJ	denotes	null
T897	2135-2142	NNS	denotes	mutants
T889	2143-2150	VBP	denotes	undergo
T899	2151-2160	NN	denotes	apoptosis
T900	2161-2163	IN	denotes	in
T901	2164-2172	NNS	denotes	neonates
T902	2173-2174	-LRB-	denotes	[
T903	2174-2175	CD	denotes	3
T904	2175-2176	-RRB-	denotes	]
T905	2176-2177	.	denotes	.
T906	2177-2350	sentence	denotes	Consistent with an apparent absence of mature hair cells, initial work suggested that all vestibular and most spiral ganglion cells are lost by postnatal day 14 (P14; [1]).
T907	2178-2188	JJ	denotes	Consistent
T909	2189-2193	IN	denotes	with
T910	2194-2196	DT	denotes	an
T912	2197-2205	JJ	denotes	apparent
T911	2206-2213	NN	denotes	absence
T913	2214-2216	IN	denotes	of
T914	2217-2223	JJ	denotes	mature
T916	2224-2228	NN	denotes	hair
T915	2229-2234	NNS	denotes	cells
T917	2234-2236	,	denotes	,
T918	2236-2243	JJ	denotes	initial
T919	2244-2248	NN	denotes	work
T908	2249-2258	VBD	denotes	suggested
T920	2259-2263	IN	denotes	that
T922	2264-2267	DT	denotes	all
T923	2268-2278	JJ	denotes	vestibular
T924	2279-2282	CC	denotes	and
T925	2283-2287	DT	denotes	most
T926	2288-2294	JJ	denotes	spiral
T928	2295-2303	NN	denotes	ganglion
T927	2304-2309	NNS	denotes	cells
T929	2310-2313	VBP	denotes	are
T921	2314-2318	VBN	denotes	lost
T930	2319-2321	IN	denotes	by
T931	2322-2331	JJ	denotes	postnatal
T932	2332-2335	NN	denotes	day
T933	2336-2338	CD	denotes	14
T934	2339-2340	-LRB-	denotes	(
T935	2340-2343	NN	denotes	P14
T936	2343-2344	:	denotes	;
T937	2345-2346	-LRB-	denotes	[
T938	2346-2347	CD	denotes	1
T939	2347-2348	-RRB-	denotes	]
T940	2348-2349	-RRB-	denotes	)
T941	2349-2350	.	denotes	.
T942	2350-2495	sentence	denotes	However, more detailed quantification by others [3] reported that at P4 about 77% of vestibular neurons and only 29% of spiral neurons are lost.
T943	2351-2358	RB	denotes	However
T945	2358-2360	,	denotes	,
T946	2360-2364	RBR	denotes	more
T948	2365-2373	JJ	denotes	detailed
T947	2374-2388	NN	denotes	quantification
T949	2389-2391	IN	denotes	by
T950	2392-2398	NNS	denotes	others
T951	2399-2400	-LRB-	denotes	[
T952	2400-2401	CD	denotes	3
T953	2401-2402	-RRB-	denotes	]
T944	2403-2411	VBD	denotes	reported
T954	2412-2416	IN	denotes	that
T956	2417-2419	IN	denotes	at
T957	2420-2422	NN	denotes	P4
T958	2423-2428	IN	denotes	about
T959	2429-2431	CD	denotes	77
T960	2431-2432	NN	denotes	%
T961	2433-2435	IN	denotes	of
T962	2436-2446	JJ	denotes	vestibular
T963	2447-2454	NNS	denotes	neurons
T964	2455-2458	CC	denotes	and
T965	2459-2463	RB	denotes	only
T966	2464-2466	CD	denotes	29
T967	2466-2467	NN	denotes	%
T968	2468-2470	IN	denotes	of
T969	2471-2477	JJ	denotes	spiral
T970	2478-2485	NNS	denotes	neurons
T971	2486-2489	VBP	denotes	are
T955	2490-2494	VBN	denotes	lost
T972	2494-2495	.	denotes	.
T973	2495-2566	sentence	denotes	It was suggested that there is possibly a complete loss in adults [3].
T974	2496-2498	PRP	denotes	It
T976	2499-2502	VBD	denotes	was
T975	2503-2512	VBN	denotes	suggested
T977	2513-2517	IN	denotes	that
T979	2518-2523	EX	denotes	there
T978	2524-2526	VBZ	denotes	is
T980	2527-2535	RB	denotes	possibly
T981	2536-2537	DT	denotes	a
T983	2538-2546	JJ	denotes	complete
T982	2547-2551	NN	denotes	loss
T984	2552-2554	IN	denotes	in
T985	2555-2561	NNS	denotes	adults
T986	2562-2563	-LRB-	denotes	[
T987	2563-2564	CD	denotes	3
T988	2564-2565	-RRB-	denotes	]
T989	2565-2566	.	denotes	.
T990	2566-2700	sentence	denotes	Other than these preliminary statements, no data exists concerning the detailed pattern of loss of innervation in Brn3c null mutants.
T991	2567-2572	RB	denotes	Other
T993	2573-2577	IN	denotes	than
T994	2578-2583	DT	denotes	these
T996	2584-2595	JJ	denotes	preliminary
T995	2596-2606	NNS	denotes	statements
T997	2606-2608	,	denotes	,
T998	2608-2610	DT	denotes	no
T999	2611-2615	NN	denotes	data
T992	2616-2622	VBZ	denotes	exists
T1000	2623-2633	VBG	denotes	concerning
T1001	2634-2637	DT	denotes	the
T1003	2638-2646	JJ	denotes	detailed
T1002	2647-2654	NN	denotes	pattern
T1004	2655-2657	IN	denotes	of
T1005	2658-2662	NN	denotes	loss
T1006	2663-2665	IN	denotes	of
T1007	2666-2677	NN	denotes	innervation
T1008	2678-2680	IN	denotes	in
T1009	2681-2686	NN	denotes	Brn3c
T1010	2687-2691	JJ	denotes	null
T1011	2692-2699	NNS	denotes	mutants
T1012	2699-2700	.	denotes	.
T1013	2700-2919	sentence	denotes	The initial development and partial differentiation of hair cells in Brn3c mutants could possibly lead to some neurotrophin expression in these cells to sustain sensory neurons through embryonic development and beyond.
T1014	2701-2704	DT	denotes	The
T1016	2705-2712	JJ	denotes	initial
T1015	2713-2724	NN	denotes	development
T1018	2725-2728	CC	denotes	and
T1019	2729-2736	JJ	denotes	partial
T1020	2737-2752	NN	denotes	differentiation
T1021	2753-2755	IN	denotes	of
T1022	2756-2760	NN	denotes	hair
T1023	2761-2766	NNS	denotes	cells
T1024	2767-2769	IN	denotes	in
T1025	2770-2775	NN	denotes	Brn3c
T1026	2776-2783	NNS	denotes	mutants
T1027	2784-2789	MD	denotes	could
T1028	2790-2798	RB	denotes	possibly
T1017	2799-2803	VB	denotes	lead
T1029	2804-2806	IN	denotes	to
T1030	2807-2811	DT	denotes	some
T1032	2812-2824	NN	denotes	neurotrophin
T1031	2825-2835	NN	denotes	expression
T1033	2836-2838	IN	denotes	in
T1034	2839-2844	DT	denotes	these
T1035	2845-2850	NNS	denotes	cells
T1036	2851-2853	TO	denotes	to
T1037	2854-2861	VB	denotes	sustain
T1038	2862-2869	JJ	denotes	sensory
T1039	2870-2877	NNS	denotes	neurons
T1040	2878-2885	IN	denotes	through
T1041	2886-2895	JJ	denotes	embryonic
T1042	2896-2907	NN	denotes	development
T1043	2908-2911	CC	denotes	and
T1044	2912-2918	RB	denotes	beyond
T1045	2918-2919	.	denotes	.
T1046	2919-3119	sentence	denotes	Data on various single and compound neurotrophin null mutants have shown that the loss of a specific neurotrophin leads to topologically restricted loss of sensory neurons in the embryonic ear [4,5].
T1047	2920-2924	NNS	denotes	Data
T1049	2925-2927	IN	denotes	on
T1050	2928-2935	JJ	denotes	various
T1052	2936-2942	JJ	denotes	single
T1054	2943-2946	CC	denotes	and
T1055	2947-2955	NN	denotes	compound
T1053	2956-2968	NN	denotes	neurotrophin
T1056	2969-2973	JJ	denotes	null
T1051	2974-2981	NNS	denotes	mutants
T1057	2982-2986	VBP	denotes	have
T1048	2987-2992	VBN	denotes	shown
T1058	2993-2997	IN	denotes	that
T1060	2998-3001	DT	denotes	the
T1061	3002-3006	NN	denotes	loss
T1062	3007-3009	IN	denotes	of
T1063	3010-3011	DT	denotes	a
T1065	3012-3020	JJ	denotes	specific
T1064	3021-3033	NN	denotes	neurotrophin
T1059	3034-3039	VBZ	denotes	leads
T1066	3040-3042	IN	denotes	to
T1067	3043-3056	RB	denotes	topologically
T1068	3057-3067	JJ	denotes	restricted
T1069	3068-3072	NN	denotes	loss
T1070	3073-3075	IN	denotes	of
T1071	3076-3083	JJ	denotes	sensory
T1072	3084-3091	NNS	denotes	neurons
T1073	3092-3094	IN	denotes	in
T1074	3095-3098	DT	denotes	the
T1076	3099-3108	JJ	denotes	embryonic
T1075	3109-3112	NN	denotes	ear
T1077	3113-3114	-LRB-	denotes	[
T1079	3114-3115	CD	denotes	4
T1080	3115-3116	,	denotes	,
T1078	3116-3117	CD	denotes	5
T1081	3117-3118	-RRB-	denotes	]
T1082	3118-3119	.	denotes	.
T1083	3119-3247	sentence	denotes	Such selective loss in Brn3c null mutants would therefore indicate reduction of a specific neurotrophin in immature hair cells.
T1084	3120-3124	JJ	denotes	Such
T1086	3125-3134	JJ	denotes	selective
T1085	3135-3139	NN	denotes	loss
T1088	3140-3142	IN	denotes	in
T1089	3143-3148	NN	denotes	Brn3c
T1091	3149-3153	JJ	denotes	null
T1090	3154-3161	NNS	denotes	mutants
T1092	3162-3167	MD	denotes	would
T1093	3168-3177	RB	denotes	therefore
T1087	3178-3186	VB	denotes	indicate
T1094	3187-3196	NN	denotes	reduction
T1095	3197-3199	IN	denotes	of
T1096	3200-3201	DT	denotes	a
T1098	3202-3210	JJ	denotes	specific
T1097	3211-3223	NN	denotes	neurotrophin
T1099	3224-3226	IN	denotes	in
T1100	3227-3235	JJ	denotes	immature
T1102	3236-3240	NN	denotes	hair
T1101	3241-3246	NNS	denotes	cells
T1103	3246-3247	.	denotes	.
T1104	3247-3388	sentence	denotes	Moreover, recent work shows that in embryos NT-3 is primarily expressed in supporting cells, moving only around birth into hair cells [6,7].
T1105	3248-3256	RB	denotes	Moreover
T1107	3256-3258	,	denotes	,
T1108	3258-3264	JJ	denotes	recent
T1109	3265-3269	NN	denotes	work
T1106	3270-3275	VBZ	denotes	shows
T1110	3276-3280	IN	denotes	that
T1112	3281-3283	IN	denotes	in
T1113	3284-3291	NNS	denotes	embryos
T1114	3292-3294	NN	denotes	NT
T1115	3294-3295	HYPH	denotes	-
T1116	3295-3296	CD	denotes	3
T1117	3297-3299	VBZ	denotes	is
T1118	3300-3309	RB	denotes	primarily
T1111	3310-3319	VBN	denotes	expressed
T1119	3320-3322	IN	denotes	in
T1120	3323-3333	VBG	denotes	supporting
T1121	3334-3339	NNS	denotes	cells
T1122	3339-3341	,	denotes	,
T1123	3341-3347	VBG	denotes	moving
T1124	3348-3352	RB	denotes	only
T1125	3353-3359	IN	denotes	around
T1126	3360-3365	NN	denotes	birth
T1127	3366-3370	IN	denotes	into
T1128	3371-3375	NN	denotes	hair
T1129	3376-3381	NNS	denotes	cells
T1130	3382-3383	-LRB-	denotes	[
T1132	3383-3384	CD	denotes	6
T1133	3384-3385	,	denotes	,
T1131	3385-3386	CD	denotes	7
T1134	3386-3387	-RRB-	denotes	]
T1135	3387-3388	.	denotes	.
T1136	3388-3666	sentence	denotes	In fact, the selective loss of vestibular as compared to cochlear sensory neurons (77% versus 29%; [3]) suggests that NT-3 expression may be less downregulated in Brn3c null mutants than BDNF [6,8,9], provided that at least some differentiation of supporting cells takes place.
T1137	3389-3391	IN	denotes	In
T1139	3392-3396	NN	denotes	fact
T1140	3396-3398	,	denotes	,
T1141	3398-3401	DT	denotes	the
T1143	3402-3411	JJ	denotes	selective
T1142	3412-3416	NN	denotes	loss
T1144	3417-3419	IN	denotes	of
T1145	3420-3430	JJ	denotes	vestibular
T1146	3431-3433	IN	denotes	as
T1147	3434-3442	VBN	denotes	compared
T1148	3443-3445	IN	denotes	to
T1149	3446-3454	JJ	denotes	cochlear
T1150	3455-3462	JJ	denotes	sensory
T1151	3463-3470	NNS	denotes	neurons
T1152	3471-3472	-LRB-	denotes	(
T1154	3472-3474	CD	denotes	77
T1153	3474-3475	NN	denotes	%
T1155	3476-3482	CC	denotes	versus
T1156	3483-3485	CD	denotes	29
T1157	3485-3486	NN	denotes	%
T1158	3486-3487	:	denotes	;
T1159	3488-3489	-LRB-	denotes	[
T1160	3489-3490	CD	denotes	3
T1161	3490-3491	-RRB-	denotes	]
T1162	3491-3492	-RRB-	denotes	)
T1138	3493-3501	VBZ	denotes	suggests
T1163	3502-3506	IN	denotes	that
T1165	3507-3509	NN	denotes	NT
T1167	3509-3510	HYPH	denotes	-
T1168	3510-3511	CD	denotes	3
T1166	3512-3522	NN	denotes	expression
T1169	3523-3526	MD	denotes	may
T1164	3527-3529	VB	denotes	be
T1170	3530-3534	RBR	denotes	less
T1171	3535-3548	VBN	denotes	downregulated
T1172	3549-3551	IN	denotes	in
T1173	3552-3557	NN	denotes	Brn3c
T1175	3558-3562	JJ	denotes	null
T1174	3563-3570	NNS	denotes	mutants
T1176	3571-3575	IN	denotes	than
T1177	3576-3580	NN	denotes	BDNF
T1178	3581-3582	-LRB-	denotes	[
T1180	3582-3583	CD	denotes	6
T1181	3583-3584	,	denotes	,
T1182	3584-3585	CD	denotes	8
T1183	3585-3586	,	denotes	,
T1179	3586-3587	CD	denotes	9
T1184	3587-3588	-RRB-	denotes	]
T1185	3588-3590	,	denotes	,
T1186	3590-3598	VBN	denotes	provided
T1187	3599-3603	IN	denotes	that
T1189	3604-3606	RB	denotes	at
T1190	3607-3612	RBS	denotes	least
T1192	3613-3617	DT	denotes	some
T1191	3618-3633	NN	denotes	differentiation
T1193	3634-3636	IN	denotes	of
T1194	3637-3647	VBG	denotes	supporting
T1195	3648-3653	NNS	denotes	cells
T1188	3654-3659	VBZ	denotes	takes
T1196	3660-3665	NN	denotes	place
T1197	3665-3666	.	denotes	.
T1198	3666-3819	sentence	denotes	In the ear [4] as well as elsewhere [10] neurotrophins are progressively downregulated in postnatal mammals and possibly replaced by other factors [11].
T1199	3667-3669	IN	denotes	In
T1201	3670-3673	DT	denotes	the
T1202	3674-3677	NN	denotes	ear
T1203	3678-3679	-LRB-	denotes	[
T1204	3679-3680	CD	denotes	4
T1205	3680-3681	-RRB-	denotes	]
T1206	3682-3684	RB	denotes	as
T1208	3685-3689	RB	denotes	well
T1207	3690-3692	IN	denotes	as
T1209	3693-3702	RB	denotes	elsewhere
T1211	3703-3704	-LRB-	denotes	[
T1210	3704-3706	CD	denotes	10
T1212	3706-3707	-RRB-	denotes	]
T1213	3708-3721	NNS	denotes	neurotrophins
T1214	3722-3725	VBP	denotes	are
T1215	3726-3739	RB	denotes	progressively
T1200	3740-3753	VBN	denotes	downregulated
T1216	3754-3756	IN	denotes	in
T1217	3757-3766	JJ	denotes	postnatal
T1218	3767-3774	NNS	denotes	mammals
T1219	3775-3778	CC	denotes	and
T1220	3779-3787	RB	denotes	possibly
T1221	3788-3796	VBN	denotes	replaced
T1222	3797-3799	IN	denotes	by
T1223	3800-3805	JJ	denotes	other
T1224	3806-3813	NNS	denotes	factors
T1225	3814-3815	-LRB-	denotes	[
T1226	3815-3817	CD	denotes	11
T1227	3817-3818	-RRB-	denotes	]
T1228	3818-3819	.	denotes	.
T1229	3819-3943	sentence	denotes	We have investigated in detail the pattern of innervation in the Brn3c mutants, as well as the expression of NT-3 and BDNF.
T1230	3820-3822	PRP	denotes	We
T1232	3823-3827	VBP	denotes	have
T1231	3828-3840	VBN	denotes	investigated
T1233	3841-3843	IN	denotes	in
T1234	3844-3850	NN	denotes	detail
T1235	3851-3854	DT	denotes	the
T1236	3855-3862	NN	denotes	pattern
T1237	3863-3865	IN	denotes	of
T1238	3866-3877	NN	denotes	innervation
T1239	3878-3880	IN	denotes	in
T1240	3881-3884	DT	denotes	the
T1242	3885-3890	NN	denotes	Brn3c
T1241	3891-3898	NNS	denotes	mutants
T1243	3898-3900	,	denotes	,
T1244	3900-3902	RB	denotes	as
T1246	3903-3907	RB	denotes	well
T1245	3908-3910	IN	denotes	as
T1247	3911-3914	DT	denotes	the
T1248	3915-3925	NN	denotes	expression
T1249	3926-3928	IN	denotes	of
T1250	3929-3931	NN	denotes	NT
T1251	3931-3932	HYPH	denotes	-
T1252	3932-3933	CD	denotes	3
T1253	3934-3937	CC	denotes	and
T1254	3938-3942	NN	denotes	BDNF
T1255	3942-3943	.	denotes	.
T1256	3943-4134	sentence	denotes	We want to evaluate a possible correlation between the topology of sensory neuron loss and absence of a specific neurotrophin or topological loss of hair cells at birth and in older animals.
T1257	3944-3946	PRP	denotes	We
T1258	3947-3951	VBP	denotes	want
T1259	3952-3954	TO	denotes	to
T1260	3955-3963	VB	denotes	evaluate
T1261	3964-3965	DT	denotes	a
T1263	3966-3974	JJ	denotes	possible
T1262	3975-3986	NN	denotes	correlation
T1264	3987-3994	IN	denotes	between
T1265	3995-3998	DT	denotes	the
T1266	3999-4007	NN	denotes	topology
T1267	4008-4010	IN	denotes	of
T1268	4011-4018	JJ	denotes	sensory
T1269	4019-4025	NN	denotes	neuron
T1270	4026-4030	NN	denotes	loss
T1271	4031-4034	CC	denotes	and
T1272	4035-4042	NN	denotes	absence
T1273	4043-4045	IN	denotes	of
T1274	4046-4047	DT	denotes	a
T1276	4048-4056	JJ	denotes	specific
T1275	4057-4069	NN	denotes	neurotrophin
T1277	4070-4072	CC	denotes	or
T1278	4073-4084	JJ	denotes	topological
T1279	4085-4089	NN	denotes	loss
T1280	4090-4092	IN	denotes	of
T1281	4093-4097	NN	denotes	hair
T1282	4098-4103	NNS	denotes	cells
T1283	4104-4106	IN	denotes	at
T1284	4107-4112	NN	denotes	birth
T1285	4113-4116	CC	denotes	and
T1286	4117-4119	IN	denotes	in
T1287	4120-4125	JJR	denotes	older
T1288	4126-4133	NNS	denotes	animals
T1289	4133-4134	.	denotes	.
T1290	4134-4260	sentence	denotes	This information could be important for an in-depth evaluation of the human deafness related to the Pou4f3 gene, DFNA15 [12].
T1291	4135-4139	DT	denotes	This
T1292	4140-4151	NN	denotes	information
T1294	4152-4157	MD	denotes	could
T1293	4158-4160	VB	denotes	be
T1295	4161-4170	JJ	denotes	important
T1296	4171-4174	IN	denotes	for
T1297	4175-4177	DT	denotes	an
T1299	4178-4180	IN	denotes	in
T1300	4180-4181	HYPH	denotes	-
T1301	4181-4186	NN	denotes	depth
T1298	4187-4197	NN	denotes	evaluation
T1302	4198-4200	IN	denotes	of
T1303	4201-4204	DT	denotes	the
T1305	4205-4210	JJ	denotes	human
T1304	4211-4219	NN	denotes	deafness
T1306	4220-4227	VBN	denotes	related
T1307	4228-4230	IN	denotes	to
T1308	4231-4234	DT	denotes	the
T1310	4235-4241	NN	denotes	Pou4f3
T1309	4242-4246	NN	denotes	gene
T1311	4246-4248	,	denotes	,
T1312	4248-4254	NN	denotes	DFNA15
T1313	4255-4256	-LRB-	denotes	[
T1314	4256-4258	CD	denotes	12
T1315	4258-4259	-RRB-	denotes	]
T1316	4259-4260	.	denotes	.
T1317	4260-4409	sentence	denotes	We report here long term retention of cochlear sensory neurons for at least 6 months, in particular in the cochlear apex, in Brn3c null mutant mice.
T1318	4261-4263	PRP	denotes	We
T1319	4264-4270	VBP	denotes	report
T1320	4271-4275	RB	denotes	here
T1321	4276-4280	JJ	denotes	long
T1322	4281-4285	NN	denotes	term
T1323	4286-4295	NN	denotes	retention
T1324	4296-4298	IN	denotes	of
T1325	4299-4307	JJ	denotes	cochlear
T1327	4308-4315	JJ	denotes	sensory
T1326	4316-4323	NNS	denotes	neurons
T1328	4324-4327	IN	denotes	for
T1329	4328-4330	RB	denotes	at
T1331	4331-4336	RBS	denotes	least
T1330	4337-4338	CD	denotes	6
T1332	4339-4345	NNS	denotes	months
T1333	4345-4347	,	denotes	,
T1334	4347-4349	IN	denotes	in
T1336	4350-4360	JJ	denotes	particular
T1335	4361-4363	IN	denotes	in
T1337	4364-4367	DT	denotes	the
T1339	4368-4376	JJ	denotes	cochlear
T1338	4377-4381	NN	denotes	apex
T1340	4381-4383	,	denotes	,
T1341	4383-4385	IN	denotes	in
T1342	4386-4391	NN	denotes	Brn3c
T1343	4392-4396	JJ	denotes	null
T1345	4397-4403	JJ	denotes	mutant
T1344	4404-4408	NNS	denotes	mice
T1346	4408-4409	.	denotes	.
T1347	4409-4628	sentence	denotes	This retention of afferents and efferents is unrelated to hair cell differentiation as not even immature hair cells can be detected at early postnatal stages with MyoVII immunocytochemistry in this part of the cochlea.
T1348	4410-4414	DT	denotes	This
T1349	4415-4424	NN	denotes	retention
T1351	4425-4427	IN	denotes	of
T1352	4428-4437	NNS	denotes	afferents
T1353	4438-4441	CC	denotes	and
T1354	4442-4451	NNS	denotes	efferents
T1350	4452-4454	VBZ	denotes	is
T1355	4455-4464	JJ	denotes	unrelated
T1356	4465-4467	IN	denotes	to
T1357	4468-4472	NN	denotes	hair
T1358	4473-4477	NN	denotes	cell
T1359	4478-4493	NN	denotes	differentiation
T1360	4494-4496	IN	denotes	as
T1362	4497-4500	RB	denotes	not
T1364	4501-4505	RB	denotes	even
T1365	4506-4514	JJ	denotes	immature
T1366	4515-4519	NN	denotes	hair
T1363	4520-4525	NNS	denotes	cells
T1367	4526-4529	MD	denotes	can
T1368	4530-4532	VB	denotes	be
T1361	4533-4541	VBN	denotes	detected
T1369	4542-4544	IN	denotes	at
T1370	4545-4550	JJ	denotes	early
T1372	4551-4560	JJ	denotes	postnatal
T1371	4561-4567	NNS	denotes	stages
T1373	4568-4572	IN	denotes	with
T1374	4573-4579	NN	denotes	MyoVII
T1375	4580-4599	NN	denotes	immunocytochemistry
T1376	4600-4602	IN	denotes	in
T1377	4603-4607	DT	denotes	this
T1378	4608-4612	NN	denotes	part
T1379	4613-4615	IN	denotes	of
T1380	4616-4619	DT	denotes	the
T1381	4620-4627	NN	denotes	cochlea
T1382	4627-4628	.	denotes	.
T1383	4628-4786	sentence	denotes	This retention of apical spiral neurons is also largely unrelated to neurotrophins which are known to be reduced in their expression in neonatal rodents [4].
T1384	4629-4633	DT	denotes	This
T1385	4634-4643	NN	denotes	retention
T1387	4644-4646	IN	denotes	of
T1388	4647-4653	JJ	denotes	apical
T1390	4654-4660	JJ	denotes	spiral
T1389	4661-4668	NNS	denotes	neurons
T1386	4669-4671	VBZ	denotes	is
T1391	4672-4676	RB	denotes	also
T1392	4677-4684	RB	denotes	largely
T1393	4685-4694	JJ	denotes	unrelated
T1394	4695-4697	IN	denotes	to
T1395	4698-4711	NNS	denotes	neurotrophins
T1396	4712-4717	WDT	denotes	which
T1398	4718-4721	VBP	denotes	are
T1397	4722-4727	VBN	denotes	known
T1399	4728-4730	TO	denotes	to
T1401	4731-4733	VB	denotes	be
T1400	4734-4741	VBN	denotes	reduced
T1402	4742-4744	IN	denotes	in
T1403	4745-4750	PRP$	denotes	their
T1404	4751-4761	NN	denotes	expression
T1405	4762-4764	IN	denotes	in
T1406	4765-4773	JJ	denotes	neonatal
T1407	4774-4781	NNS	denotes	rodents
T1408	4782-4783	-LRB-	denotes	[
T1409	4783-4784	CD	denotes	4
T1410	4784-4785	-RRB-	denotes	]
T1411	4785-4786	.	denotes	.
T1476	4796-4798	TO	denotes	To
T1477	4799-4809	VB	denotes	appreciate
T1479	4810-4813	DT	denotes	the
T1480	4814-4821	NNS	denotes	effects
T1481	4822-4824	IN	denotes	of
T1482	4825-4828	DT	denotes	the
T1484	4829-4834	NN	denotes	Brn3c
T1485	4835-4839	JJ	denotes	null
T1483	4840-4848	NN	denotes	mutation
T1486	4849-4851	IN	denotes	on
T1487	4852-4855	DT	denotes	the
T1488	4856-4863	NN	denotes	pattern
T1489	4864-4866	IN	denotes	of
T1490	4867-4870	DT	denotes	the
T1492	4871-4876	JJ	denotes	inner
T1493	4877-4880	NN	denotes	ear
T1491	4881-4892	NN	denotes	innervation
T1494	4892-4894	,	denotes	,
T1495	4894-4896	PRP	denotes	we
T1496	4897-4902	RB	denotes	first
T1478	4903-4907	VBP	denotes	want
T1497	4908-4910	TO	denotes	to
T1498	4911-4918	VB	denotes	present
T1499	4919-4922	DT	denotes	the
T1500	4923-4930	NNS	denotes	effects
T1501	4931-4933	IN	denotes	of
T1502	4934-4938	NN	denotes	BDNF
T1504	4939-4942	CC	denotes	and
T1505	4943-4945	NN	denotes	NT
T1506	4945-4946	HYPH	denotes	-
T1507	4946-4947	CD	denotes	3
T1508	4948-4952	JJ	denotes	null
T1503	4953-4962	NNS	denotes	mutations
T1509	4963-4965	IN	denotes	at
T1510	4966-4971	NN	denotes	birth
T1511	4972-4973	-LRB-	denotes	[
T1513	4973-4974	CD	denotes	6
T1514	4974-4975	,	denotes	,
T1515	4975-4977	CD	denotes	13
T1516	4977-4978	,	denotes	,
T1512	4978-4980	CD	denotes	14
T1517	4980-4981	-RRB-	denotes	]
T1518	4981-4982	.	denotes	.
T1519	4982-5159	sentence	denotes	Null mutants of BDNF or its receptor trkB lose all innervation to the semicircular canals and have a reduced innervation to the utricle, saccule and apical turn of the cochlea.
T1520	4983-4987	JJ	denotes	Null
T1521	4988-4995	NNS	denotes	mutants
T1523	4996-4998	IN	denotes	of
T1524	4999-5003	NN	denotes	BDNF
T1525	5004-5006	CC	denotes	or
T1526	5007-5010	PRP$	denotes	its
T1527	5011-5019	NN	denotes	receptor
T1528	5020-5024	NN	denotes	trkB
T1522	5025-5029	VBP	denotes	lose
T1529	5030-5033	DT	denotes	all
T1530	5034-5045	NN	denotes	innervation
T1531	5046-5048	IN	denotes	to
T1532	5049-5052	DT	denotes	the
T1534	5053-5065	JJ	denotes	semicircular
T1533	5066-5072	NNS	denotes	canals
T1535	5073-5076	CC	denotes	and
T1536	5077-5081	VBP	denotes	have
T1537	5082-5083	DT	denotes	a
T1539	5084-5091	VBN	denotes	reduced
T1538	5092-5103	NN	denotes	innervation
T1540	5104-5106	IN	denotes	to
T1541	5107-5110	DT	denotes	the
T1542	5111-5118	NN	denotes	utricle
T1543	5118-5120	,	denotes	,
T1544	5120-5127	NN	denotes	saccule
T1545	5128-5131	CC	denotes	and
T1546	5132-5138	JJ	denotes	apical
T1547	5139-5143	NN	denotes	turn
T1548	5144-5146	IN	denotes	of
T1549	5147-5150	DT	denotes	the
T1550	5151-5158	NN	denotes	cochlea
T1551	5158-5159	.	denotes	.
T1552	5159-5356	sentence	denotes	In contrast, null mutations of either NT-3 or its receptor trkC result in loss of spiral neurons in the basal turn with formation of an inner spiral bundle of afferents extending to the basal tip.
T1553	5160-5162	IN	denotes	In
T1555	5163-5171	NN	denotes	contrast
T1556	5171-5173	,	denotes	,
T1557	5173-5177	JJ	denotes	null
T1558	5178-5187	NNS	denotes	mutations
T1559	5188-5190	IN	denotes	of
T1560	5191-5197	CC	denotes	either
T1561	5198-5200	NN	denotes	NT
T1562	5200-5201	HYPH	denotes	-
T1563	5201-5202	CD	denotes	3
T1564	5203-5205	CC	denotes	or
T1565	5206-5209	PRP$	denotes	its
T1566	5210-5218	NN	denotes	receptor
T1567	5219-5223	NN	denotes	trkC
T1554	5224-5230	VBP	denotes	result
T1568	5231-5233	IN	denotes	in
T1569	5234-5238	NN	denotes	loss
T1570	5239-5241	IN	denotes	of
T1571	5242-5248	JJ	denotes	spiral
T1572	5249-5256	NNS	denotes	neurons
T1573	5257-5259	IN	denotes	in
T1574	5260-5263	DT	denotes	the
T1576	5264-5269	JJ	denotes	basal
T1575	5270-5274	NN	denotes	turn
T1577	5275-5279	IN	denotes	with
T1578	5280-5289	NN	denotes	formation
T1579	5290-5292	IN	denotes	of
T1580	5293-5295	DT	denotes	an
T1582	5296-5301	JJ	denotes	inner
T1583	5302-5308	JJ	denotes	spiral
T1581	5309-5315	NN	denotes	bundle
T1584	5316-5318	IN	denotes	of
T1585	5319-5328	NNS	denotes	afferents
T1586	5329-5338	VBG	denotes	extending
T1587	5339-5341	IN	denotes	to
T1588	5342-5345	DT	denotes	the
T1590	5346-5351	JJ	denotes	basal
T1589	5352-5355	NN	denotes	tip
T1591	5355-5356	.	denotes	.
T1592	5356-5547	sentence	denotes	Our null hypothesis for this study would be that Brn3c null mice show severe compromised production of these neurotrophins and should therefore show a comparable pattern of nerve fiber loss.
T1593	5357-5360	PRP$	denotes	Our
T1595	5361-5365	JJ	denotes	null
T1594	5366-5376	NN	denotes	hypothesis
T1597	5377-5380	IN	denotes	for
T1598	5381-5385	DT	denotes	this
T1599	5386-5391	NN	denotes	study
T1600	5392-5397	MD	denotes	would
T1596	5398-5400	VB	denotes	be
T1601	5401-5405	IN	denotes	that
T1603	5406-5411	NN	denotes	Brn3c
T1605	5412-5416	JJ	denotes	null
T1604	5417-5421	NNS	denotes	mice
T1602	5422-5426	VBP	denotes	show
T1606	5427-5433	JJ	denotes	severe
T1608	5434-5445	VBN	denotes	compromised
T1607	5446-5456	NN	denotes	production
T1609	5457-5459	IN	denotes	of
T1610	5460-5465	DT	denotes	these
T1611	5466-5479	NNS	denotes	neurotrophins
T1612	5480-5483	CC	denotes	and
T1613	5484-5490	MD	denotes	should
T1615	5491-5500	RB	denotes	therefore
T1614	5501-5505	VB	denotes	show
T1616	5506-5507	DT	denotes	a
T1618	5508-5518	JJ	denotes	comparable
T1617	5519-5526	NN	denotes	pattern
T1619	5527-5529	IN	denotes	of
T1620	5530-5535	NN	denotes	nerve
T1621	5536-5541	NN	denotes	fiber
T1622	5542-5546	NN	denotes	loss
T1623	5546-5547	.	denotes	.
T1849	5568-5570	IN	denotes	at
T1850	5571-5576	NN	denotes	birth
T1851	5577-5578	-LRB-	denotes	(
T1852	5578-5580	NN	denotes	P0
T1853	5580-5581	-RRB-	denotes	)
T1854	5581-5753	sentence	denotes	Vestibular ganglia are smaller in Brn3c null mutants (Fig. 1b) than in control littermates (Fig. 1a), but larger than in BDNF or trkB null mutants of the same age [13,15].
T1855	5582-5592	JJ	denotes	Vestibular
T1856	5593-5600	NNS	denotes	ganglia
T1857	5601-5604	VBP	denotes	are
T1858	5605-5612	JJR	denotes	smaller
T1859	5613-5615	IN	denotes	in
T1860	5616-5621	NN	denotes	Brn3c
T1862	5622-5626	JJ	denotes	null
T1861	5627-5634	NNS	denotes	mutants
T1863	5635-5636	-LRB-	denotes	(
T1865	5636-5640	NN	denotes	Fig.
T1864	5641-5643	NN	denotes	1b
T1866	5643-5644	-RRB-	denotes	)
T1867	5645-5649	IN	denotes	than
T1868	5650-5652	IN	denotes	in
T1869	5653-5660	NN	denotes	control
T1870	5661-5672	NNS	denotes	littermates
T1871	5673-5674	-LRB-	denotes	(
T1873	5674-5678	NN	denotes	Fig.
T1872	5679-5681	NN	denotes	1a
T1874	5681-5682	-RRB-	denotes	)
T1875	5682-5684	,	denotes	,
T1876	5684-5687	CC	denotes	but
T1877	5688-5694	JJR	denotes	larger
T1878	5695-5699	IN	denotes	than
T1879	5700-5702	IN	denotes	in
T1880	5703-5707	NN	denotes	BDNF
T1882	5708-5710	CC	denotes	or
T1883	5711-5715	NN	denotes	trkB
T1881	5716-5720	JJ	denotes	null
T1884	5721-5728	NNS	denotes	mutants
T1885	5729-5731	IN	denotes	of
T1886	5732-5735	DT	denotes	the
T1888	5736-5740	JJ	denotes	same
T1887	5741-5744	NN	denotes	age
T1889	5745-5746	-LRB-	denotes	[
T1891	5746-5748	CD	denotes	13
T1892	5748-5749	,	denotes	,
T1890	5749-5751	CD	denotes	15
T1893	5751-5752	-RRB-	denotes	]
T1894	5752-5753	.	denotes	.
T1895	5753-5878	sentence	denotes	The reduction in apparent size of the vestibular ganglia is in agreement with quantitative data published previously [3,13].
T1896	5754-5757	DT	denotes	The
T1897	5758-5767	NN	denotes	reduction
T1899	5768-5770	IN	denotes	in
T1900	5771-5779	JJ	denotes	apparent
T1901	5780-5784	NN	denotes	size
T1902	5785-5787	IN	denotes	of
T1903	5788-5791	DT	denotes	the
T1905	5792-5802	JJ	denotes	vestibular
T1904	5803-5810	NNS	denotes	ganglia
T1898	5811-5813	VBZ	denotes	is
T1906	5814-5816	IN	denotes	in
T1907	5817-5826	NN	denotes	agreement
T1908	5827-5831	IN	denotes	with
T1909	5832-5844	JJ	denotes	quantitative
T1910	5845-5849	NNS	denotes	data
T1911	5850-5859	VBN	denotes	published
T1912	5860-5870	RB	denotes	previously
T1913	5871-5872	-LRB-	denotes	[
T1915	5872-5873	CD	denotes	3
T1916	5873-5874	,	denotes	,
T1914	5874-5876	CD	denotes	13
T1917	5876-5877	-RRB-	denotes	]
T1918	5877-5878	.	denotes	.
T1919	5878-6050	sentence	denotes	These data suggest a loss of 80–85% of vestibular sensory neurons in BDNF and trkB null mutants [13] and of 77% of vestibular sensory neurons in P4 Brn3c null mutants [3].
T1920	5879-5884	DT	denotes	These
T1921	5885-5889	NNS	denotes	data
T1922	5890-5897	VBP	denotes	suggest
T1923	5898-5899	DT	denotes	a
T1924	5900-5904	NN	denotes	loss
T1925	5905-5907	IN	denotes	of
T1926	5908-5910	CD	denotes	80
T1928	5910-5911	SYM	denotes	–
T1927	5911-5913	CD	denotes	85
T1929	5913-5914	NN	denotes	%
T1930	5915-5917	IN	denotes	of
T1931	5918-5928	JJ	denotes	vestibular
T1933	5929-5936	JJ	denotes	sensory
T1932	5937-5944	NNS	denotes	neurons
T1934	5945-5947	IN	denotes	in
T1935	5948-5952	NN	denotes	BDNF
T1937	5953-5956	CC	denotes	and
T1938	5957-5961	NN	denotes	trkB
T1936	5962-5966	JJ	denotes	null
T1939	5967-5974	NNS	denotes	mutants
T1940	5975-5976	-LRB-	denotes	[
T1941	5976-5978	CD	denotes	13
T1942	5978-5979	-RRB-	denotes	]
T1943	5980-5983	CC	denotes	and
T1944	5984-5986	IN	denotes	of
T1945	5987-5989	CD	denotes	77
T1946	5989-5990	NN	denotes	%
T1947	5991-5993	IN	denotes	of
T1948	5994-6004	JJ	denotes	vestibular
T1950	6005-6012	JJ	denotes	sensory
T1949	6013-6020	NNS	denotes	neurons
T1951	6021-6023	IN	denotes	in
T1952	6024-6026	NN	denotes	P4
T1953	6027-6032	NN	denotes	Brn3c
T1954	6033-6037	JJ	denotes	null
T1955	6038-6045	NNS	denotes	mutants
T1956	6046-6047	-LRB-	denotes	[
T1957	6047-6048	CD	denotes	3
T1958	6048-6049	-RRB-	denotes	]
T1959	6049-6050	.	denotes	.
T1960	6050-6189	sentence	denotes	Thus, the size reduction in the vestibular sensory ganglion could be compatible with a loss of BDNF production in the immature hair cells.
T1961	6051-6055	RB	denotes	Thus
T1963	6055-6057	,	denotes	,
T1964	6057-6060	DT	denotes	the
T1966	6061-6065	NN	denotes	size
T1965	6066-6075	NN	denotes	reduction
T1967	6076-6078	IN	denotes	in
T1968	6079-6082	DT	denotes	the
T1970	6083-6093	JJ	denotes	vestibular
T1971	6094-6101	JJ	denotes	sensory
T1969	6102-6110	NN	denotes	ganglion
T1972	6111-6116	MD	denotes	could
T1962	6117-6119	VB	denotes	be
T1973	6120-6130	JJ	denotes	compatible
T1974	6131-6135	IN	denotes	with
T1975	6136-6137	DT	denotes	a
T1976	6138-6142	NN	denotes	loss
T1977	6143-6145	IN	denotes	of
T1978	6146-6150	NN	denotes	BDNF
T1979	6151-6161	NN	denotes	production
T1980	6162-6164	IN	denotes	in
T1981	6165-6168	DT	denotes	the
T1983	6169-6177	JJ	denotes	immature
T1984	6178-6182	NN	denotes	hair
T1982	6183-6188	NNS	denotes	cells
T1985	6188-6189	.	denotes	.
T1986	6189-6190	sentence	denotes
T8591	6200-6204	NN	denotes	Size
T8592	6205-6215	NNS	denotes	variations
T8593	6216-6218	IN	denotes	of
T8594	6219-6229	JJ	denotes	vestibular
T8595	6230-6237	NNS	denotes	ganglia
T8596	6238-6240	IN	denotes	in
T8597	6241-6248	NN	denotes	control
T8599	6249-6252	CC	denotes	and
T8600	6253-6259	JJ	denotes	mutant
T8598	6260-6271	NNS	denotes	littermates
T8601	6272-6279	VBN	denotes	labeled
T8602	6280-6284	IN	denotes	with
T8603	6285-6288	NN	denotes	DiI
T8604	6288-6289	.	denotes	.
T8605	6289-6423	sentence	denotes	In newborn animals, the vestibular ganglion shows a dramatic reduction in Brn3c null mutants (b) compared to control littermates (a).
T8606	6290-6292	IN	denotes	In
T8608	6293-6300	JJ	denotes	newborn
T8609	6301-6308	NNS	denotes	animals
T8610	6308-6310	,	denotes	,
T8611	6310-6313	DT	denotes	the
T8613	6314-6324	JJ	denotes	vestibular
T8612	6325-6333	NN	denotes	ganglion
T8607	6334-6339	VBZ	denotes	shows
T8614	6340-6341	DT	denotes	a
T8616	6342-6350	JJ	denotes	dramatic
T8615	6351-6360	NN	denotes	reduction
T8617	6361-6363	IN	denotes	in
T8618	6364-6369	NN	denotes	Brn3c
T8619	6370-6374	JJ	denotes	null
T8620	6375-6382	NNS	denotes	mutants
T8621	6383-6384	-LRB-	denotes	(
T8622	6384-6385	NN	denotes	b
T8623	6385-6386	-RRB-	denotes	)
T8624	6387-6395	VBN	denotes	compared
T8625	6396-6398	IN	denotes	to
T8626	6399-6406	NN	denotes	control
T8627	6407-6418	NNS	denotes	littermates
T8628	6419-6420	-LRB-	denotes	(
T8629	6420-6421	DT	denotes	a
T8630	6421-6422	-RRB-	denotes	)
T8631	6422-6423	.	denotes	.
T8632	6423-6890	sentence	denotes	Abbreviations for this and other figures: AC, anterior crista; ggl, ganglion; c, spiral capillary; Genic. ggl., geniculate ganglion; GER, greater epithelial ridge; HC, horizontal crista; HaC, vestibular hair cell; IHC, inner hair cell; iHC, immature hair cell; IGSB, intraganglionic spiral bundle; OHC, outer hair cell; PC, posterior crista; S, saccule; SG, spiral ganglion; TM, tectorial membrane; U, utricle; VCN, ventral cochlear nucleus; VG, vestibular ganglion.
T8633	6424-6437	NNS	denotes	Abbreviations
T8635	6438-6441	IN	denotes	for
T8636	6442-6446	DT	denotes	this
T8637	6447-6450	CC	denotes	and
T8638	6451-6456	JJ	denotes	other
T8639	6457-6464	NNS	denotes	figures
T8640	6464-6466	:	denotes	:
T8634	6466-6468	NN	denotes	AC
T8641	6468-6470	,	denotes	,
T8642	6470-6478	JJ	denotes	anterior
T8643	6479-6485	NN	denotes	crista
T8644	6485-6486	:	denotes	;
T8645	6487-6490	NN	denotes	ggl
T8646	6490-6492	,	denotes	,
T8647	6492-6500	NN	denotes	ganglion
T8648	6500-6501	:	denotes	;
T8649	6502-6503	NN	denotes	c
T8650	6503-6505	,	denotes	,
T8651	6505-6511	JJ	denotes	spiral
T8652	6512-6521	NN	denotes	capillary
T8653	6521-6522	:	denotes	;
T8654	6523-6529	NNP	denotes	Genic.
T8655	6530-6534	NNP	denotes	ggl.
T8656	6534-6536	,	denotes	,
T8657	6536-6546	JJ	denotes	geniculate
T8658	6547-6555	NN	denotes	ganglion
T8659	6555-6556	:	denotes	;
T8660	6557-6560	NN	denotes	GER
T8661	6560-6562	,	denotes	,
T8662	6562-6569	JJR	denotes	greater
T8664	6570-6580	JJ	denotes	epithelial
T8663	6581-6586	NN	denotes	ridge
T8665	6586-6587	:	denotes	;
T8666	6588-6590	NN	denotes	HC
T8667	6590-6592	,	denotes	,
T8668	6592-6602	JJ	denotes	horizontal
T8669	6603-6609	NN	denotes	crista
T8670	6609-6610	:	denotes	;
T8671	6611-6614	NN	denotes	HaC
T8672	6614-6616	,	denotes	,
T8673	6616-6626	JJ	denotes	vestibular
T8675	6627-6631	NN	denotes	hair
T8674	6632-6636	NN	denotes	cell
T8676	6636-6637	:	denotes	;
T8677	6638-6641	NN	denotes	IHC
T8678	6641-6643	,	denotes	,
T8679	6643-6648	JJ	denotes	inner
T8681	6649-6653	NN	denotes	hair
T8680	6654-6658	NN	denotes	cell
T8682	6658-6659	:	denotes	;
T8683	6660-6663	NN	denotes	iHC
T8684	6663-6665	,	denotes	,
T8685	6665-6673	JJ	denotes	immature
T8687	6674-6678	NN	denotes	hair
T8686	6679-6683	NN	denotes	cell
T8688	6683-6684	:	denotes	;
T8689	6685-6689	NN	denotes	IGSB
T8690	6689-6691	,	denotes	,
T8691	6691-6706	JJ	denotes	intraganglionic
T8693	6707-6713	JJ	denotes	spiral
T8692	6714-6720	NN	denotes	bundle
T8694	6720-6721	:	denotes	;
T8695	6722-6725	NN	denotes	OHC
T8696	6725-6727	,	denotes	,
T8697	6727-6732	JJ	denotes	outer
T8699	6733-6737	NN	denotes	hair
T8698	6738-6742	NN	denotes	cell
T8700	6742-6743	:	denotes	;
T8701	6744-6746	NN	denotes	PC
T8702	6746-6748	,	denotes	,
T8703	6748-6757	JJ	denotes	posterior
T8704	6758-6764	NN	denotes	crista
T8705	6764-6765	:	denotes	;
T8706	6766-6767	NN	denotes	S
T8707	6767-6769	,	denotes	,
T8708	6769-6776	NN	denotes	saccule
T8709	6776-6777	:	denotes	;
T8710	6778-6780	NN	denotes	SG
T8711	6780-6782	,	denotes	,
T8712	6782-6788	JJ	denotes	spiral
T8713	6789-6797	NN	denotes	ganglion
T8714	6797-6798	:	denotes	;
T8715	6799-6801	NN	denotes	TM
T8716	6801-6803	,	denotes	,
T8717	6803-6812	JJ	denotes	tectorial
T8718	6813-6821	NN	denotes	membrane
T8719	6821-6822	:	denotes	;
T8720	6823-6824	NN	denotes	U
T8721	6824-6826	,	denotes	,
T8722	6826-6833	NN	denotes	utricle
T8723	6833-6834	:	denotes	;
T8724	6835-6838	NN	denotes	VCN
T8725	6838-6840	,	denotes	,
T8726	6840-6847	JJ	denotes	ventral
T8728	6848-6856	JJ	denotes	cochlear
T8727	6857-6864	NN	denotes	nucleus
T8729	6864-6865	:	denotes	;
T8730	6866-6868	NN	denotes	VG
T8731	6868-6870	,	denotes	,
T8732	6870-6880	JJ	denotes	vestibular
T8733	6881-6889	NN	denotes	ganglion
T8734	6889-6890	.	denotes	.
T8735	6890-6913	sentence	denotes	Bar indicates 1000 μm.
T8736	6891-6894	NNP	denotes	Bar
T8737	6895-6904	VBZ	denotes	indicates
T8738	6905-6909	CD	denotes	1000
T8739	6910-6912	NNS	denotes	μm
T8740	6912-6913	.	denotes	.
T1988	6914-6915	NN	denotes	H
T1987	6914-6916	sentence	denotes	Ho
T1989	6915-6916	JJ	denotes	o
T1991	6916-6921	RB	denotes	wever
T1990	6916-7042	sentence	denotes	wever, the Brn3c null mutants show only a reduced density of afferent and efferent fibers to all vestibular sensory epithelia.
T1993	6921-6923	,	denotes	,
T1994	6923-6926	DT	denotes	the
T1996	6927-6932	NN	denotes	Brn3c
T1997	6933-6937	JJ	denotes	null
T1995	6938-6945	NNS	denotes	mutants
T1992	6946-6950	VBP	denotes	show
T1998	6951-6955	RB	denotes	only
T2000	6956-6957	DT	denotes	a
T2001	6958-6965	VBN	denotes	reduced
T1999	6966-6973	NN	denotes	density
T2002	6974-6976	IN	denotes	of
T2003	6977-6985	JJ	denotes	afferent
T2005	6986-6989	CC	denotes	and
T2006	6990-6998	JJ	denotes	efferent
T2004	6999-7005	NNS	denotes	fibers
T2007	7006-7008	IN	denotes	to
T2008	7009-7012	DT	denotes	all
T2010	7013-7023	JJ	denotes	vestibular
T2011	7024-7031	JJ	denotes	sensory
T2009	7032-7041	NNS	denotes	epithelia
T2012	7041-7042	.	denotes	.
T2013	7042-7197	sentence	denotes	There is no specific loss of all afferent and efferent innervation to the canal crista (Fig. 2a), a hallmark of both BDNF and trkB null mutations [13,15].
T2014	7043-7048	EX	denotes	There
T2015	7049-7051	VBZ	denotes	is
T2016	7052-7054	DT	denotes	no
T2018	7055-7063	JJ	denotes	specific
T2017	7064-7068	NN	denotes	loss
T2019	7069-7071	IN	denotes	of
T2020	7072-7075	DT	denotes	all
T2022	7076-7084	JJ	denotes	afferent
T2023	7085-7088	CC	denotes	and
T2024	7089-7097	JJ	denotes	efferent
T2021	7098-7109	NN	denotes	innervation
T2025	7110-7112	IN	denotes	to
T2026	7113-7116	DT	denotes	the
T2028	7117-7122	JJ	denotes	canal
T2027	7123-7129	NN	denotes	crista
T2029	7130-7131	-LRB-	denotes	(
T2031	7131-7135	NN	denotes	Fig.
T2030	7136-7138	NN	denotes	2a
T2032	7138-7139	-RRB-	denotes	)
T2033	7139-7141	,	denotes	,
T2034	7141-7142	DT	denotes	a
T2035	7143-7151	NN	denotes	hallmark
T2036	7152-7154	IN	denotes	of
T2037	7155-7159	CC	denotes	both
T2038	7160-7164	NN	denotes	BDNF
T2040	7165-7168	CC	denotes	and
T2041	7169-7173	NN	denotes	trkB
T2039	7174-7178	JJ	denotes	null
T2042	7179-7188	NNS	denotes	mutations
T2043	7189-7190	-LRB-	denotes	[
T2045	7190-7192	CD	denotes	13
T2046	7192-7193	,	denotes	,
T2044	7193-7195	CD	denotes	15
T2047	7195-7196	-RRB-	denotes	]
T2048	7196-7197	.	denotes	.
T2049	7197-7408	sentence	denotes	In fact, the reduction of fibers seems to be rather uniform throughout a given sensory epithelium with the crista innervation being qualitatively no more reduced than the innervation of the utricle and saccule.
T2050	7198-7200	IN	denotes	In
T2052	7201-7205	NN	denotes	fact
T2053	7205-7207	,	denotes	,
T2054	7207-7210	DT	denotes	the
T2055	7211-7220	NN	denotes	reduction
T2056	7221-7223	IN	denotes	of
T2057	7224-7230	NNS	denotes	fibers
T2051	7231-7236	VBZ	denotes	seems
T2058	7237-7239	TO	denotes	to
T2059	7240-7242	VB	denotes	be
T2060	7243-7249	RB	denotes	rather
T2061	7250-7257	JJ	denotes	uniform
T2062	7258-7268	IN	denotes	throughout
T2063	7269-7270	DT	denotes	a
T2065	7271-7276	VBN	denotes	given
T2066	7277-7284	JJ	denotes	sensory
T2064	7285-7295	NN	denotes	epithelium
T2067	7296-7300	IN	denotes	with
T2068	7301-7304	DT	denotes	the
T2070	7305-7311	NN	denotes	crista
T2069	7312-7323	NN	denotes	innervation
T2071	7324-7329	VBG	denotes	being
T2072	7330-7343	RB	denotes	qualitatively
T2073	7344-7346	DT	denotes	no
T2074	7347-7351	RBR	denotes	more
T2075	7352-7359	VBN	denotes	reduced
T2076	7360-7364	IN	denotes	than
T2077	7365-7368	DT	denotes	the
T2078	7369-7380	NN	denotes	innervation
T2079	7381-7383	IN	denotes	of
T2080	7384-7387	DT	denotes	the
T2081	7388-7395	NN	denotes	utricle
T2082	7396-7399	CC	denotes	and
T2083	7400-7407	NN	denotes	saccule
T2084	7407-7408	.	denotes	.
T2085	7408-7538	sentence	denotes	No loss in specific areas, comparable to that in BDNF null mutant mice, is apparent in the saccule or utricle of Brn3c null mice.
T2086	7409-7411	DT	denotes	No
T2087	7412-7416	NN	denotes	loss
T2089	7417-7419	IN	denotes	in
T2090	7420-7428	JJ	denotes	specific
T2091	7429-7434	NNS	denotes	areas
T2092	7434-7436	,	denotes	,
T2093	7436-7446	JJ	denotes	comparable
T2094	7447-7449	IN	denotes	to
T2095	7450-7454	DT	denotes	that
T2096	7455-7457	IN	denotes	in
T2097	7458-7462	NN	denotes	BDNF
T2098	7463-7467	JJ	denotes	null
T2100	7468-7474	JJ	denotes	mutant
T2099	7475-7479	NNS	denotes	mice
T2101	7479-7481	,	denotes	,
T2088	7481-7483	VBZ	denotes	is
T2102	7484-7492	JJ	denotes	apparent
T2103	7493-7495	IN	denotes	in
T2104	7496-7499	DT	denotes	the
T2105	7500-7507	NN	denotes	saccule
T2106	7508-7510	CC	denotes	or
T2107	7511-7518	NN	denotes	utricle
T2108	7519-7521	IN	denotes	of
T2109	7522-7527	NN	denotes	Brn3c
T2110	7528-7532	JJ	denotes	null
T2111	7533-7537	NNS	denotes	mice
T2112	7537-7538	.	denotes	.
T2113	7538-7639	sentence	denotes	Similar patterns of innervation are obtained using acteylated tubulin immunocytochemistry (Fig. 2b).
T2114	7539-7546	JJ	denotes	Similar
T2115	7547-7555	NNS	denotes	patterns
T2117	7556-7558	IN	denotes	of
T2118	7559-7570	NN	denotes	innervation
T2119	7571-7574	VBP	denotes	are
T2116	7575-7583	VBN	denotes	obtained
T2120	7584-7589	VBG	denotes	using
T2121	7590-7600	VBN	denotes	acteylated
T2122	7601-7608	NN	denotes	tubulin
T2123	7609-7628	NN	denotes	immunocytochemistry
T2124	7629-7630	-LRB-	denotes	(
T2126	7630-7634	NN	denotes	Fig.
T2125	7635-7637	NN	denotes	2b
T2127	7637-7638	-RRB-	denotes	)
T2128	7638-7639	.	denotes	.
T2129	7639-7640	sentence	denotes
T8782	7650-7661	NN	denotes	Innervation
T8784	7662-7664	IN	denotes	of
T8785	7665-7670	NN	denotes	Brn3c
T8787	7671-7675	JJ	denotes	null
T8789	7676-7679	CC	denotes	and
T8788	7680-7687	NN	denotes	control
T8786	7688-7692	NNS	denotes	ears
T8790	7693-7696	VBP	denotes	are
T8783	7697-7702	VBN	denotes	shown
T8791	7703-7706	IN	denotes	for
T8792	7707-7714	JJ	denotes	newborn
T8793	7715-7719	NNS	denotes	mice
T8794	7719-7720	.	denotes	.
T8795	7720-7862	sentence	denotes	There is no specific loss of fibers to any vestibular endorgan, as visualized by DiI labeling (a) or acetylated tubulin immunoreactivity (b).
T8796	7721-7726	EX	denotes	There
T8797	7727-7729	VBZ	denotes	is
T8798	7730-7732	DT	denotes	no
T8800	7733-7741	JJ	denotes	specific
T8799	7742-7746	NN	denotes	loss
T8801	7747-7749	IN	denotes	of
T8802	7750-7756	NNS	denotes	fibers
T8803	7757-7759	IN	denotes	to
T8804	7760-7763	DT	denotes	any
T8806	7764-7774	JJ	denotes	vestibular
T8805	7775-7783	NN	denotes	endorgan
T8807	7783-7785	,	denotes	,
T8808	7785-7787	IN	denotes	as
T8809	7788-7798	VBN	denotes	visualized
T8810	7799-7801	IN	denotes	by
T8811	7802-7805	NN	denotes	DiI
T8812	7806-7814	NN	denotes	labeling
T8813	7815-7816	-LRB-	denotes	(
T8814	7816-7817	DT	denotes	a
T8815	7817-7818	-RRB-	denotes	)
T8816	7819-7821	CC	denotes	or
T8817	7822-7832	VBN	denotes	acetylated
T8818	7833-7840	NN	denotes	tubulin
T8819	7841-7857	NN	denotes	immunoreactivity
T8820	7858-7859	-LRB-	denotes	(
T8821	7859-7860	NN	denotes	b
T8822	7860-7861	-RRB-	denotes	)
T8823	7861-7862	.	denotes	.
T8824	7862-8023	sentence	denotes	No major differences in pattern of projection through radial fibers are found in the cochlea of Brn3c null mutants (d,f) as compared to control littermates (c).
T8825	7863-7865	DT	denotes	No
T8827	7866-7871	JJ	denotes	major
T8826	7872-7883	NNS	denotes	differences
T8829	7884-7886	IN	denotes	in
T8830	7887-7894	NN	denotes	pattern
T8831	7895-7897	IN	denotes	of
T8832	7898-7908	NN	denotes	projection
T8833	7909-7916	IN	denotes	through
T8834	7917-7923	NN	denotes	radial
T8835	7924-7930	NNS	denotes	fibers
T8836	7931-7934	VBP	denotes	are
T8828	7935-7940	VBN	denotes	found
T8837	7941-7943	IN	denotes	in
T8838	7944-7947	DT	denotes	the
T8839	7948-7955	NN	denotes	cochlea
T8840	7956-7958	IN	denotes	of
T8841	7959-7964	NN	denotes	Brn3c
T8843	7965-7969	JJ	denotes	null
T8842	7970-7977	NNS	denotes	mutants
T8844	7978-7979	-LRB-	denotes	(
T8846	7979-7980	NN	denotes	d
T8847	7980-7981	,	denotes	,
T8845	7981-7982	NN	denotes	f
T8848	7982-7983	-RRB-	denotes	)
T8849	7984-7986	IN	denotes	as
T8850	7987-7995	VBN	denotes	compared
T8851	7996-7998	IN	denotes	to
T8852	7999-8006	NN	denotes	control
T8853	8007-8018	NNS	denotes	littermates
T8854	8019-8020	-LRB-	denotes	(
T8855	8020-8021	NN	denotes	c
T8856	8021-8022	-RRB-	denotes	)
T8857	8022-8023	.	denotes	.
T8858	8023-8106	sentence	denotes	Note, however, the lack of orderly fiber outgrowth to the outer hair cells (c, d).
T8859	8024-8028	VB	denotes	Note
T8860	8028-8030	,	denotes	,
T8861	8030-8037	RB	denotes	however
T8862	8037-8039	,	denotes	,
T8863	8039-8042	DT	denotes	the
T8864	8043-8047	NN	denotes	lack
T8865	8048-8050	IN	denotes	of
T8866	8051-8058	JJ	denotes	orderly
T8868	8059-8064	NN	denotes	fiber
T8867	8065-8074	NN	denotes	outgrowth
T8869	8075-8077	IN	denotes	to
T8870	8078-8081	DT	denotes	the
T8872	8082-8087	JJ	denotes	outer
T8873	8088-8092	NN	denotes	hair
T8871	8093-8098	NNS	denotes	cells
T8874	8099-8100	-LRB-	denotes	(
T8876	8100-8101	NN	denotes	c
T8877	8101-8103	,	denotes	,
T8875	8103-8104	NN	denotes	d
T8878	8104-8105	-RRB-	denotes	)
T8879	8105-8106	.	denotes	.
T8880	8106-8249	sentence	denotes	Efferent fibers to the ear show a well developed intraganglionic spiral bundle (IGSB) with no detectable differences compared to controls (e).
T8881	8107-8115	JJ	denotes	Efferent
T8882	8116-8122	NNS	denotes	fibers
T8884	8123-8125	IN	denotes	to
T8885	8126-8129	DT	denotes	the
T8886	8130-8133	NN	denotes	ear
T8883	8134-8138	VBP	denotes	show
T8887	8139-8140	DT	denotes	a
T8889	8141-8145	RB	denotes	well
T8890	8146-8155	VBN	denotes	developed
T8891	8156-8171	JJ	denotes	intraganglionic
T8892	8172-8178	JJ	denotes	spiral
T8888	8179-8185	NN	denotes	bundle
T8893	8186-8187	-LRB-	denotes	(
T8894	8187-8191	NN	denotes	IGSB
T8895	8191-8192	-RRB-	denotes	)
T8896	8193-8197	IN	denotes	with
T8897	8198-8200	DT	denotes	no
T8899	8201-8211	JJ	denotes	detectable
T8898	8212-8223	NNS	denotes	differences
T8900	8224-8232	VBN	denotes	compared
T8901	8233-8235	IN	denotes	to
T8902	8236-8244	NNS	denotes	controls
T8903	8245-8246	-LRB-	denotes	(
T8904	8246-8247	NN	denotes	e
T8905	8247-8248	-RRB-	denotes	)
T8906	8248-8249	.	denotes	.
T8907	8249-8271	sentence	denotes	Bar indicates 100 μm.
T8908	8250-8253	NNP	denotes	Bar
T8909	8254-8263	VBZ	denotes	indicates
T8910	8264-8267	CD	denotes	100
T8911	8268-8270	NNS	denotes	μm
T8912	8270-8271	.	denotes	.
T2131	8272-8282	JJ	denotes	Consistent
T2130	8272-8487	sentence	denotes	Consistent with the finding of Xiang et al. [3] of only a 29% loss of spiral sensory neurons at P4, our data show little difference in the pattern of innervation of the cochlea in P0 Brn3c null mutants (Fig. 2c,2d).
T2133	8283-8287	IN	denotes	with
T2134	8288-8291	DT	denotes	the
T2135	8292-8299	NN	denotes	finding
T2136	8300-8302	IN	denotes	of
T2137	8303-8308	NNP	denotes	Xiang
T2138	8309-8311	FW	denotes	et
T2139	8312-8315	FW	denotes	al.
T2140	8316-8317	-LRB-	denotes	[
T2141	8317-8318	CD	denotes	3
T2142	8318-8319	-RRB-	denotes	]
T2143	8320-8322	IN	denotes	of
T2144	8323-8327	RB	denotes	only
T2146	8328-8329	DT	denotes	a
T2147	8330-8332	CD	denotes	29
T2148	8332-8333	NN	denotes	%
T2145	8334-8338	NN	denotes	loss
T2149	8339-8341	IN	denotes	of
T2150	8342-8348	JJ	denotes	spiral
T2152	8349-8356	JJ	denotes	sensory
T2151	8357-8364	NNS	denotes	neurons
T2153	8365-8367	IN	denotes	at
T2154	8368-8370	NN	denotes	P4
T2155	8370-8372	,	denotes	,
T2156	8372-8375	PRP$	denotes	our
T2157	8376-8380	NNS	denotes	data
T2132	8381-8385	VBP	denotes	show
T2158	8386-8392	JJ	denotes	little
T2159	8393-8403	NN	denotes	difference
T2160	8404-8406	IN	denotes	in
T2161	8407-8410	DT	denotes	the
T2162	8411-8418	NN	denotes	pattern
T2163	8419-8421	IN	denotes	of
T2164	8422-8433	NN	denotes	innervation
T2165	8434-8436	IN	denotes	of
T2166	8437-8440	DT	denotes	the
T2167	8441-8448	NN	denotes	cochlea
T2168	8449-8451	IN	denotes	in
T2169	8452-8454	NN	denotes	P0
T2171	8455-8460	NN	denotes	Brn3c
T2172	8461-8465	JJ	denotes	null
T2170	8466-8473	NNS	denotes	mutants
T2173	8474-8475	-LRB-	denotes	(
T2175	8475-8479	NNP	denotes	Fig.
T2176	8480-8482	NN	denotes	2c
T2177	8482-8483	,	denotes	,
T2174	8483-8485	NN	denotes	2d
T2178	8485-8486	-RRB-	denotes	)
T2179	8486-8487	.	denotes	.
T2180	8487-8624	sentence	denotes	No selective loss of spiral neurons is observed in Brn3c null mutants in the basal turn, a feature of either NT-3 or trkC loss [6,9,14].
T2181	8488-8490	DT	denotes	No
T2183	8491-8500	JJ	denotes	selective
T2182	8501-8505	NN	denotes	loss
T2185	8506-8508	IN	denotes	of
T2186	8509-8515	JJ	denotes	spiral
T2187	8516-8523	NNS	denotes	neurons
T2188	8524-8526	VBZ	denotes	is
T2184	8527-8535	VBN	denotes	observed
T2189	8536-8538	IN	denotes	in
T2190	8539-8544	NN	denotes	Brn3c
T2192	8545-8549	JJ	denotes	null
T2191	8550-8557	NNS	denotes	mutants
T2193	8558-8560	IN	denotes	in
T2194	8561-8564	DT	denotes	the
T2196	8565-8570	JJ	denotes	basal
T2195	8571-8575	NN	denotes	turn
T2197	8575-8577	,	denotes	,
T2198	8577-8578	DT	denotes	a
T2199	8579-8586	NN	denotes	feature
T2200	8587-8589	IN	denotes	of
T2201	8590-8596	CC	denotes	either
T2202	8597-8599	NN	denotes	NT
T2204	8599-8600	HYPH	denotes	-
T2205	8600-8601	CD	denotes	3
T2206	8602-8604	CC	denotes	or
T2207	8605-8609	NN	denotes	trkC
T2203	8610-8614	NN	denotes	loss
T2208	8615-8616	-LRB-	denotes	[
T2210	8616-8617	CD	denotes	6
T2211	8617-8618	,	denotes	,
T2212	8618-8619	CD	denotes	9
T2213	8619-8620	,	denotes	,
T2209	8620-8622	CD	denotes	14
T2214	8622-8623	-RRB-	denotes	]
T2215	8623-8624	.	denotes	.
T2216	8624-8844	sentence	denotes	Likewise, the innervation of the apex (Fig. 2f) shows no detectable abnormality in overall pattern of innervation compared to control animals (data not shown), an indication that BDNF could be expressed in the apex [6].
T2217	8625-8633	RB	denotes	Likewise
T2219	8633-8635	,	denotes	,
T2220	8635-8638	DT	denotes	the
T2221	8639-8650	NN	denotes	innervation
T2222	8651-8653	IN	denotes	of
T2223	8654-8657	DT	denotes	the
T2224	8658-8662	NN	denotes	apex
T2225	8663-8664	-LRB-	denotes	(
T2227	8664-8668	NN	denotes	Fig.
T2226	8669-8671	NN	denotes	2f
T2228	8671-8672	-RRB-	denotes	)
T2218	8673-8678	VBZ	denotes	shows
T2229	8679-8681	DT	denotes	no
T2231	8682-8692	JJ	denotes	detectable
T2230	8693-8704	NN	denotes	abnormality
T2232	8705-8707	IN	denotes	in
T2233	8708-8715	JJ	denotes	overall
T2234	8716-8723	NN	denotes	pattern
T2235	8724-8726	IN	denotes	of
T2236	8727-8738	NN	denotes	innervation
T2237	8739-8747	VBN	denotes	compared
T2238	8748-8750	IN	denotes	to
T2239	8751-8758	NN	denotes	control
T2240	8759-8766	NNS	denotes	animals
T2241	8767-8768	-LRB-	denotes	(
T2243	8768-8772	NNS	denotes	data
T2244	8773-8776	RB	denotes	not
T2242	8777-8782	VBN	denotes	shown
T2245	8782-8783	-RRB-	denotes	)
T2246	8783-8785	,	denotes	,
T2247	8785-8787	DT	denotes	an
T2248	8788-8798	NN	denotes	indication
T2249	8799-8803	IN	denotes	that
T2251	8804-8808	NN	denotes	BDNF
T2252	8809-8814	MD	denotes	could
T2253	8815-8817	VB	denotes	be
T2250	8818-8827	VBN	denotes	expressed
T2254	8828-8830	IN	denotes	in
T2255	8831-8834	DT	denotes	the
T2256	8835-8839	NN	denotes	apex
T2257	8840-8841	-LRB-	denotes	[
T2258	8841-8842	CD	denotes	6
T2259	8842-8843	-RRB-	denotes	]
T2260	8843-8844	.	denotes	.
T2261	8844-9039	sentence	denotes	In addition, the pattern of efferent innervation shows no deviation from normal either (Fig. 2e), whereas they show the same pattern of loss as afferent fibers in neurotrophin null mutants [16].
T2262	8845-8847	IN	denotes	In
T2264	8848-8856	NN	denotes	addition
T2265	8856-8858	,	denotes	,
T2266	8858-8861	DT	denotes	the
T2267	8862-8869	NN	denotes	pattern
T2268	8870-8872	IN	denotes	of
T2269	8873-8881	JJ	denotes	efferent
T2270	8882-8893	NN	denotes	innervation
T2263	8894-8899	VBZ	denotes	shows
T2271	8900-8902	DT	denotes	no
T2272	8903-8912	NN	denotes	deviation
T2273	8913-8917	IN	denotes	from
T2274	8918-8924	JJ	denotes	normal
T2275	8925-8931	RB	denotes	either
T2276	8932-8933	-LRB-	denotes	(
T2278	8933-8937	NNP	denotes	Fig.
T2277	8938-8940	NNP	denotes	2e
T2279	8940-8941	-RRB-	denotes	)
T2280	8941-8943	,	denotes	,
T2281	8943-8950	IN	denotes	whereas
T2283	8951-8955	PRP	denotes	they
T2282	8956-8960	VBP	denotes	show
T2284	8961-8964	DT	denotes	the
T2286	8965-8969	JJ	denotes	same
T2285	8970-8977	NN	denotes	pattern
T2287	8978-8980	IN	denotes	of
T2288	8981-8985	NN	denotes	loss
T2289	8986-8988	IN	denotes	as
T2290	8989-8997	JJ	denotes	afferent
T2291	8998-9004	NNS	denotes	fibers
T2292	9005-9007	IN	denotes	in
T2293	9008-9020	NN	denotes	neurotrophin
T2295	9021-9025	JJ	denotes	null
T2294	9026-9033	NNS	denotes	mutants
T2296	9034-9035	-LRB-	denotes	[
T2297	9035-9037	CD	denotes	16
T2298	9037-9038	-RRB-	denotes	]
T2299	9038-9039	.	denotes	.
T2300	9039-9190	sentence	denotes	These data suggest that the spiral sensory neurons develop qualitatively normal at least until P0 and therefore allow normal pathfinding of efferents.
T2301	9040-9045	DT	denotes	These
T2302	9046-9050	NNS	denotes	data
T2303	9051-9058	VBP	denotes	suggest
T2304	9059-9063	IN	denotes	that
T2306	9064-9067	DT	denotes	the
T2308	9068-9074	JJ	denotes	spiral
T2309	9075-9082	JJ	denotes	sensory
T2307	9083-9090	NNS	denotes	neurons
T2305	9091-9098	VBP	denotes	develop
T2310	9099-9112	RB	denotes	qualitatively
T2311	9113-9119	JJ	denotes	normal
T2312	9120-9122	RB	denotes	at
T2313	9123-9128	RBS	denotes	least
T2314	9129-9134	IN	denotes	until
T2315	9135-9137	NN	denotes	P0
T2316	9138-9141	CC	denotes	and
T2317	9142-9151	RB	denotes	therefore
T2318	9152-9157	VBP	denotes	allow
T2319	9158-9164	JJ	denotes	normal
T2320	9165-9176	NN	denotes	pathfinding
T2321	9177-9179	IN	denotes	of
T2322	9180-9189	NNS	denotes	efferents
T2323	9189-9190	.	denotes	.
T2324	9190-9316	sentence	denotes	Most interestingly, there is no increase in radial fiber spacing in the apex, a specific problem of BDNF null mutants [6,13].
T2325	9191-9195	RBS	denotes	Most
T2326	9196-9209	RB	denotes	interestingly
T2328	9209-9211	,	denotes	,
T2329	9211-9216	EX	denotes	there
T2327	9217-9219	VBZ	denotes	is
T2330	9220-9222	DT	denotes	no
T2331	9223-9231	NN	denotes	increase
T2332	9232-9234	IN	denotes	in
T2333	9235-9241	JJ	denotes	radial
T2334	9242-9247	NN	denotes	fiber
T2335	9248-9255	NN	denotes	spacing
T2336	9256-9258	IN	denotes	in
T2337	9259-9262	DT	denotes	the
T2338	9263-9267	NN	denotes	apex
T2339	9267-9269	,	denotes	,
T2340	9269-9270	DT	denotes	a
T2342	9271-9279	JJ	denotes	specific
T2341	9280-9287	NN	denotes	problem
T2343	9288-9290	IN	denotes	of
T2344	9291-9295	NN	denotes	BDNF
T2346	9296-9300	JJ	denotes	null
T2345	9301-9308	NNS	denotes	mutants
T2347	9309-9310	-LRB-	denotes	[
T2349	9310-9311	CD	denotes	6
T2350	9311-9312	,	denotes	,
T2348	9312-9314	CD	denotes	13
T2351	9314-9315	-RRB-	denotes	]
T2352	9315-9316	.	denotes	.
T2353	9316-9416	sentence	denotes	However, there is one qualitative difference not recognized in any single neurotrophin null mutant.
T2354	9317-9324	RB	denotes	However
T2356	9324-9326	,	denotes	,
T2357	9326-9331	EX	denotes	there
T2355	9332-9334	VBZ	denotes	is
T2358	9335-9338	CD	denotes	one
T2360	9339-9350	JJ	denotes	qualitative
T2359	9351-9361	NN	denotes	difference
T2361	9362-9365	RB	denotes	not
T2362	9366-9376	VBN	denotes	recognized
T2363	9377-9379	IN	denotes	in
T2364	9380-9383	DT	denotes	any
T2366	9384-9390	JJ	denotes	single
T2367	9391-9403	NN	denotes	neurotrophin
T2368	9404-9408	JJ	denotes	null
T2365	9409-9415	NN	denotes	mutant
T2369	9415-9416	.	denotes	.
T2370	9416-9783	sentence	denotes	Afferents reach all three rows of outer hair cells in the basal turn of control wildtype littermates (Fig. 2c), but both afferent and efferent outgrowth is disorganized to outer hair cells in Brn3c null mutants (Fig. 2d) and does not show any clear organization into three distinct longitudinal fiber bundles paralleling the three rows in the outer hair cell region.
T2371	9417-9426	NNS	denotes	Afferents
T2372	9427-9432	VBP	denotes	reach
T2373	9433-9436	DT	denotes	all
T2375	9437-9442	CD	denotes	three
T2374	9443-9447	NNS	denotes	rows
T2376	9448-9450	IN	denotes	of
T2377	9451-9456	JJ	denotes	outer
T2379	9457-9461	NN	denotes	hair
T2378	9462-9467	NNS	denotes	cells
T2380	9468-9470	IN	denotes	in
T2381	9471-9474	DT	denotes	the
T2383	9475-9480	JJ	denotes	basal
T2382	9481-9485	NN	denotes	turn
T2384	9486-9488	IN	denotes	of
T2385	9489-9496	NN	denotes	control
T2387	9497-9505	JJ	denotes	wildtype
T2386	9506-9517	NNS	denotes	littermates
T2388	9518-9519	-LRB-	denotes	(
T2390	9519-9523	NN	denotes	Fig.
T2389	9524-9526	NN	denotes	2c
T2391	9526-9527	-RRB-	denotes	)
T2392	9527-9529	,	denotes	,
T2393	9529-9532	CC	denotes	but
T2394	9533-9537	CC	denotes	both
T2395	9538-9546	JJ	denotes	afferent
T2397	9547-9550	CC	denotes	and
T2398	9551-9559	JJ	denotes	efferent
T2396	9560-9569	NN	denotes	outgrowth
T2399	9570-9572	VBZ	denotes	is
T2400	9573-9585	JJ	denotes	disorganized
T2401	9586-9588	IN	denotes	to
T2402	9589-9594	JJ	denotes	outer
T2404	9595-9599	NN	denotes	hair
T2403	9600-9605	NNS	denotes	cells
T2405	9606-9608	IN	denotes	in
T2406	9609-9614	NN	denotes	Brn3c
T2407	9615-9619	JJ	denotes	null
T2408	9620-9627	NNS	denotes	mutants
T2409	9628-9629	-LRB-	denotes	(
T2411	9629-9633	NNP	denotes	Fig.
T2410	9634-9636	NNP	denotes	2d
T2412	9636-9637	-RRB-	denotes	)
T2413	9638-9641	CC	denotes	and
T2414	9642-9646	VBZ	denotes	does
T2416	9647-9650	RB	denotes	not
T2415	9651-9655	VB	denotes	show
T2417	9656-9659	DT	denotes	any
T2419	9660-9665	JJ	denotes	clear
T2418	9666-9678	NN	denotes	organization
T2420	9679-9683	IN	denotes	into
T2421	9684-9689	CD	denotes	three
T2423	9690-9698	JJ	denotes	distinct
T2424	9699-9711	JJ	denotes	longitudinal
T2425	9712-9717	NN	denotes	fiber
T2422	9718-9725	NNS	denotes	bundles
T2426	9726-9737	VBG	denotes	paralleling
T2427	9738-9741	DT	denotes	the
T2429	9742-9747	CD	denotes	three
T2428	9748-9752	NNS	denotes	rows
T2430	9753-9755	IN	denotes	in
T2431	9756-9759	DT	denotes	the
T2433	9760-9765	JJ	denotes	outer
T2434	9766-9770	NN	denotes	hair
T2435	9771-9775	NN	denotes	cell
T2432	9776-9782	NN	denotes	region
T2436	9782-9783	.	denotes	.
T2437	9783-9898	sentence	denotes	These data suggest that fiber organization in the outer hair cell region is partly disrupted in Brn3c null mutant.
T2438	9784-9789	DT	denotes	These
T2439	9790-9794	NNS	denotes	data
T2440	9795-9802	VBP	denotes	suggest
T2441	9803-9807	IN	denotes	that
T2443	9808-9813	NN	denotes	fiber
T2444	9814-9826	NN	denotes	organization
T2445	9827-9829	IN	denotes	in
T2446	9830-9833	DT	denotes	the
T2448	9834-9839	JJ	denotes	outer
T2449	9840-9844	NN	denotes	hair
T2450	9845-9849	NN	denotes	cell
T2447	9850-9856	NN	denotes	region
T2451	9857-9859	VBZ	denotes	is
T2452	9860-9866	RB	denotes	partly
T2442	9867-9876	VBN	denotes	disrupted
T2453	9877-9879	IN	denotes	in
T2454	9880-9885	NN	denotes	Brn3c
T2455	9886-9890	JJ	denotes	null
T2456	9891-9897	NN	denotes	mutant
T2457	9897-9898	.	denotes	.
R10	T197	T196	punct	", ",retention
R100	T300	T298	prep	of,reduction
R1001	T1849	T1848	prep	at,"mutant mice show long-term, incomplete retention of some afferent inner ear innervation Abstract Background Ears of Brn3c null mutants develop immature hair cells, identifiable only by certain molecular markers, and undergo apoptosis in neonates. This partial development of hair cells could lead to enough neurotrophin expression to sustain sensory neurons through embryonic development. We have therefore investigated in these mutants the patterns of innervation and of expression of known neurotrophins. Results At birth there is a limited expression of BDNF and NT-3 in the mutant sensory epithelia and DiI tracing shows no specific reduction of afferents or efferents that resembles neurotrophin null mutations. At postnatal day 7/8 (P7/8), innervation is severely reduced both qualitatively and quantitatively. 1% of myosin VIIa-positive immature hair cells are present in the mutant cochlea, concentrated in the base. Around 20% of immature hair cells exist in the mutant vestibular sensory epithelia. Despite more severe loss of hair cells (1% compared to 20%), the cochlea retains many more sensory neurons (46% compared to 15%) than vestibular epithelia. Even 6 months old mutant mice have some fibers to all vestibular sensory epithelia and many more to the cochlear apex which lacks MyoVIIa positive hair cells. Topologically organized central cochlea projections exist at least until P8, suggesting that functional hair cells are not required to establish such projections. Conclusion The limited expression of neurotrophins in the cochlea of Brn3c null mice suffices to support many sensory neurons, particularly in the cochlea, until birth. The molecular nature of the long term survival of apical spiral neurons remains unclear. Background Brn3c is a POU domain factor that is crucial for inner ear hair cell development. Targeted null Brn3c mutants have no mature hair cells [1,2]. Close examination has revealed that some 'immature' hair cells form in Brn3c null mutants and express cellular markers such as Myosin VI and VIIa, calretinin and parvalbumin [3]. Furthermore, these immature hair cells of Brn3c null mutants undergo apoptosis in neonates [3]. Consistent with an apparent absence of mature hair cells, initial work suggested that all vestibular and most spiral ganglion cells are lost by postnatal day 14 (P14; [1]). However, more detailed quantification by others [3] reported that at P4 about 77% of vestibular neurons and only 29% of spiral neurons are lost. It was suggested that there is possibly a complete loss in adults [3]. Other than these preliminary statements, no data exists concerning the detailed pattern of loss of innervation in Brn3c null mutants. The initial development and partial differentiation of hair cells in Brn3c mutants could possibly lead to some neurotrophin expression in these cells to sustain sensory neurons through embryonic development and beyond. Data on various single and compound neurotrophin null mutants have shown that the loss of a specific neurotrophin leads to topologically restricted loss of sensory neurons in the embryonic ear [4,5]. Such selective loss in Brn3c null mutants would therefore indicate reduction of a specific neurotrophin in immature hair cells. Moreover, recent work shows that in embryos NT-3 is primarily expressed in supporting cells, moving only around birth into hair cells [6,7]. In fact, the selective loss of vestibular as compared to cochlear sensory neurons (77% versus 29%; [3]) suggests that NT-3 expression may be less downregulated in Brn3c null mutants than BDNF [6,8,9], provided that at least some differentiation of supporting cells takes place. In the ear [4] as well as elsewhere [10] neurotrophins are progressively downregulated in postnatal mammals and possibly replaced by other factors [11]. We have investigated in detail the pattern of innervation in the Brn3c mutants, as well as the expression of NT-3 and BDNF. We want to evaluate a possible correlation between the topology of sensory neuron loss and absence of a specific neurotrophin or topological loss of hair cells at birth and in older animals. This information could be important for an in-depth evaluation of the human deafness related to the Pou4f3 gene, DFNA15 [12]. We report here long term retention of cochlear sensory neurons for at least 6 months, in particular in the cochlear apex, in Brn3c null mutant mice. This retention of afferents and efferents is unrelated to hair cell differentiation as not even immature hair cells can be detected at early postnatal stages with MyoVII immunocytochemistry in this part of the cochlea. This retention of apical spiral neurons is also largely unrelated to neurotrophins which are known to be reduced in their expression in neonatal rodents [4]. Results To appreciate the effects of the Brn3c null mutation on the pattern of the inner ear innervation, we first want to present the effects of BDNF and NT-3 null mutations at birth [6,13,14]. Null mutants of BDNF or its receptor trkB lose all innervation to the semicircular canals and have a reduced innervation to the utricle, saccule and apical turn of the cochlea. In contrast, null mutations of either NT-3 or its receptor trkC result in loss of spiral neurons in the basal turn with formation of an inner spiral bundle of afferents extending to the basal tip. Our null hypothesis for this study would be that Brn3c null mice show severe compromised production of these neurotrophins and should therefore show a comparable pattern of nerve fiber loss. Brn3c null mutants"
R1002	T1850	T1849	pobj	birth,at
R1003	T1851	T1850	punct	(,birth
R1004	T1852	T1850	appos	P0,birth
R1005	T1853	T1850	punct	),birth
R1006	T1855	T1856	amod	Vestibular,ganglia
R1007	T1856	T1857	nsubj	ganglia,are
R1008	T1858	T1857	acomp	smaller,are
R1009	T1859	T1858	prep	in,smaller
R101	T301	T300	pobj	afferents,of
R1010	T1860	T1861	nmod	Brn3c,mutants
R1011	T1861	T1859	pobj	mutants,in
R1012	T1862	T1861	amod	null,mutants
R1013	T1863	T1864	punct	(,1b
R1014	T1864	T1858	parataxis	1b,smaller
R1015	T1865	T1864	compound	Fig.,1b
R1016	T1866	T1864	punct	),1b
R1017	T1867	T1858	prep	than,smaller
R1018	T1868	T1867	prep	in,than
R1019	T1869	T1870	compound	control,littermates
R102	T302	T301	cc	or,afferents
R1020	T1870	T1868	pobj	littermates,in
R1021	T1871	T1872	punct	(,1a
R1022	T1872	T1868	parataxis	1a,in
R1023	T1873	T1872	compound	Fig.,1a
R1024	T1874	T1872	punct	),1a
R1025	T1875	T1858	punct	", ",smaller
R1026	T1876	T1858	cc	but,smaller
R1027	T1877	T1858	conj	larger,smaller
R1028	T1878	T1877	prep	than,larger
R1029	T1879	T1878	prep	in,than
R103	T303	T301	conj	efferents,afferents
R1030	T1880	T1881	npadvmod	BDNF,null
R1031	T1881	T1884	amod	null,mutants
R1032	T1882	T1880	cc	or,BDNF
R1033	T1883	T1880	conj	trkB,BDNF
R1034	T1884	T1879	pobj	mutants,in
R1035	T1885	T1884	prep	of,mutants
R1036	T1886	T1887	det	the,age
R1037	T1887	T1885	pobj	age,of
R1038	T1888	T1887	amod	same,age
R1039	T1889	T1890	punct	[,15
R104	T304	T305	dep	that,resembles
R1040	T1890	T1857	parataxis	15,are
R1041	T1891	T1890	nummod	13,15
R1042	T1892	T1890	punct	",",15
R1043	T1893	T1890	punct	],15
R1044	T1894	T1857	punct	.,are
R1045	T1896	T1897	det	The,reduction
R1046	T1897	T1898	nsubj	reduction,is
R1047	T1899	T1897	prep	in,reduction
R1048	T1900	T1901	amod	apparent,size
R1049	T1901	T1899	pobj	size,in
R105	T305	T301	relcl	resembles,afferents
R1050	T1902	T1897	prep	of,reduction
R1051	T1903	T1904	det	the,ganglia
R1052	T1904	T1902	pobj	ganglia,of
R1053	T1905	T1904	amod	vestibular,ganglia
R1054	T1906	T1898	prep	in,is
R1055	T1907	T1906	pobj	agreement,in
R1056	T1908	T1907	prep	with,agreement
R1057	T1909	T1910	amod	quantitative,data
R1058	T1910	T1908	pobj	data,with
R1059	T1911	T1910	acl	published,data
R106	T306	T307	nmod	neurotrophin,mutations
R1060	T1912	T1911	advmod	previously,published
R1061	T1913	T1914	punct	[,13
R1062	T1914	T1898	parataxis	13,is
R1063	T1915	T1914	nummod	3,13
R1064	T1916	T1914	punct	",",13
R1065	T1917	T1914	punct	],13
R1066	T1918	T1898	punct	.,is
R1067	T1920	T1921	det	These,data
R1068	T1921	T1922	nsubj	data,suggest
R1069	T1923	T1924	det	a,loss
R107	T307	T305	dobj	mutations,resembles
R1070	T1924	T1922	dobj	loss,suggest
R1071	T1925	T1924	prep	of,loss
R1072	T1926	T1927	quantmod	80,85
R1073	T1927	T1929	nummod	85,%
R1074	T1928	T1927	punct	–,85
R1075	T1929	T1925	pobj	%,of
R1076	T1930	T1929	prep	of,%
R1077	T1931	T1932	amod	vestibular,neurons
R1078	T1932	T1930	pobj	neurons,of
R1079	T1933	T1932	amod	sensory,neurons
R108	T308	T307	amod	null,mutations
R1080	T1934	T1929	prep	in,%
R1081	T1935	T1936	npadvmod	BDNF,null
R1082	T1936	T1939	amod	null,mutants
R1083	T1937	T1935	cc	and,BDNF
R1084	T1938	T1935	conj	trkB,BDNF
R1085	T1939	T1934	pobj	mutants,in
R1086	T1940	T1941	punct	[,13
R1087	T1941	T1939	parataxis	13,mutants
R1088	T1942	T1941	punct	],13
R1089	T1943	T1925	cc	and,of
R109	T309	T276	punct	.,is
R1090	T1944	T1925	conj	of,of
R1091	T1945	T1946	nummod	77,%
R1092	T1946	T1944	pobj	%,of
R1093	T1947	T1946	prep	of,%
R1094	T1948	T1949	amod	vestibular,neurons
R1095	T1949	T1947	pobj	neurons,of
R1096	T1950	T1949	amod	sensory,neurons
R1097	T1951	T1946	prep	in,%
R1098	T1952	T1953	compound	P4,Brn3c
R1099	T1953	T1954	npadvmod	Brn3c,null
R11	T198	T196	amod	incomplete,retention
R110	T311	T312	prep	At,reduced
R1100	T1954	T1955	amod	null,mutants
R1101	T1955	T1951	pobj	mutants,in
R1102	T1956	T1957	punct	[,3
R1103	T1957	T1955	parataxis	3,mutants
R1104	T1958	T1957	punct	],3
R1105	T1959	T1922	punct	.,suggest
R1106	T1961	T1962	advmod	Thus,be
R1107	T1963	T1962	punct	", ",be
R1108	T1964	T1965	det	the,reduction
R1109	T1965	T1962	nsubj	reduction,be
R111	T313	T314	amod	postnatal,day
R1110	T1966	T1965	compound	size,reduction
R1111	T1967	T1965	prep	in,reduction
R1112	T1968	T1969	det	the,ganglion
R1113	T1969	T1967	pobj	ganglion,in
R1114	T1970	T1969	amod	vestibular,ganglion
R1115	T1971	T1969	amod	sensory,ganglion
R1116	T1972	T1962	aux	could,be
R1117	T1973	T1962	acomp	compatible,be
R1118	T1974	T1973	prep	with,compatible
R1119	T1975	T1976	det	a,loss
R112	T314	T311	pobj	day,At
R1120	T1976	T1974	pobj	loss,with
R1121	T1977	T1976	prep	of,loss
R1122	T1978	T1979	compound	BDNF,production
R1123	T1979	T1977	pobj	production,of
R1124	T1980	T1976	prep	in,loss
R1125	T1981	T1982	det	the,cells
R1126	T1982	T1980	pobj	cells,in
R1127	T1983	T1982	amod	immature,cells
R1128	T1984	T1982	compound	hair,cells
R1129	T1985	T1962	punct	.,be
R113	T315	T314	nummod	7,day
R1130	T1991	T1992	advmod	wever,show
R1131	T1993	T1992	punct	", ",show
R1132	T1994	T1995	det	the,mutants
R1133	T1995	T1992	nsubj	mutants,show
R1134	T1996	T1995	nmod	Brn3c,mutants
R1135	T1997	T1995	amod	null,mutants
R1136	T1998	T1999	advmod	only,density
R1137	T1999	T1992	dobj	density,show
R1138	T2000	T1999	det	a,density
R1139	T2001	T1999	amod	reduced,density
R114	T316	T314	punct	/,day
R1140	T2002	T1999	prep	of,density
R1141	T2003	T2004	amod	afferent,fibers
R1142	T2004	T2002	pobj	fibers,of
R1143	T2005	T2003	cc	and,afferent
R1144	T2006	T2003	conj	efferent,afferent
R1145	T2007	T1992	prep	to,show
R1146	T2008	T2009	det	all,epithelia
R1147	T2009	T2007	pobj	epithelia,to
R1148	T2010	T2009	amod	vestibular,epithelia
R1149	T2011	T2009	amod	sensory,epithelia
R115	T317	T314	nummod	8,day
R1150	T2012	T1992	punct	.,show
R1151	T2014	T2015	expl	There,is
R1152	T2016	T2017	det	no,loss
R1153	T2017	T2015	attr	loss,is
R1154	T2018	T2017	amod	specific,loss
R1155	T2019	T2017	prep	of,loss
R1156	T2020	T2021	det	all,innervation
R1157	T2021	T2019	pobj	innervation,of
R1158	T2022	T2021	amod	afferent,innervation
R1159	T2023	T2022	cc	and,afferent
R116	T318	T314	punct	(,day
R1160	T2024	T2022	conj	efferent,afferent
R1161	T2025	T2017	prep	to,loss
R1162	T2026	T2027	det	the,crista
R1163	T2027	T2025	pobj	crista,to
R1164	T2028	T2027	amod	canal,crista
R1165	T2029	T2030	punct	(,2a
R1166	T2030	T2027	parataxis	2a,crista
R1167	T2031	T2030	compound	Fig.,2a
R1168	T2032	T2030	punct	),2a
R1169	T2033	T2017	punct	", ",loss
R117	T319	T314	appos	P7,day
R1170	T2034	T2035	det	a,hallmark
R1171	T2035	T2017	appos	hallmark,loss
R1172	T2036	T2035	prep	of,hallmark
R1173	T2037	T2038	preconj	both,BDNF
R1174	T2038	T2039	npadvmod	BDNF,null
R1175	T2039	T2042	amod	null,mutations
R1176	T2040	T2038	cc	and,BDNF
R1177	T2041	T2038	conj	trkB,BDNF
R1178	T2042	T2036	pobj	mutations,of
R1179	T2043	T2044	punct	[,15
R118	T320	T319	punct	/,P7
R1180	T2044	T2017	parataxis	15,loss
R1181	T2045	T2044	nummod	13,15
R1182	T2046	T2044	punct	",",15
R1183	T2047	T2044	punct	],15
R1184	T2048	T2015	punct	.,is
R1185	T2050	T2051	prep	In,seems
R1186	T2052	T2050	pobj	fact,In
R1187	T2053	T2051	punct	", ",seems
R1188	T2054	T2055	det	the,reduction
R1189	T2055	T2051	nsubj	reduction,seems
R119	T321	T319	nummod	8,P7
R1190	T2056	T2055	prep	of,reduction
R1191	T2057	T2056	pobj	fibers,of
R1192	T2058	T2059	aux	to,be
R1193	T2059	T2051	xcomp	be,seems
R1194	T2060	T2061	advmod	rather,uniform
R1195	T2061	T2059	acomp	uniform,be
R1196	T2062	T2059	prep	throughout,be
R1197	T2063	T2064	det	a,epithelium
R1198	T2064	T2062	pobj	epithelium,throughout
R1199	T2065	T2064	amod	given,epithelium
R12	T199	T196	prep	of,retention
R120	T322	T312	punct	),reduced
R1200	T2066	T2064	amod	sensory,epithelium
R1201	T2067	T2059	prep	with,be
R1202	T2068	T2069	det	the,innervation
R1203	T2069	T2071	nsubj	innervation,being
R1204	T2070	T2069	compound	crista,innervation
R1205	T2071	T2067	pcomp	being,with
R1206	T2072	T2071	advmod	qualitatively,being
R1207	T2073	T2074	neg	no,more
R1208	T2074	T2075	advmod	more,reduced
R1209	T2075	T2071	acomp	reduced,being
R121	T323	T312	punct	", ",reduced
R1210	T2076	T2075	prep	than,reduced
R1211	T2077	T2078	det	the,innervation
R1212	T2078	T2076	pobj	innervation,than
R1213	T2079	T2078	prep	of,innervation
R1214	T2080	T2081	det	the,utricle
R1215	T2081	T2079	pobj	utricle,of
R1216	T2082	T2081	cc	and,utricle
R1217	T2083	T2081	conj	saccule,utricle
R1218	T2084	T2051	punct	.,seems
R1219	T2086	T2087	det	No,loss
R122	T324	T312	nsubjpass	innervation,reduced
R1220	T2087	T2088	nsubj	loss,is
R1221	T2089	T2087	prep	in,loss
R1222	T2090	T2091	amod	specific,areas
R1223	T2091	T2089	pobj	areas,in
R1224	T2092	T2087	punct	", ",loss
R1225	T2093	T2087	amod	comparable,loss
R1226	T2094	T2093	prep	to,comparable
R1227	T2095	T2094	pobj	that,to
R1228	T2096	T2095	prep	in,that
R1229	T2097	T2098	npadvmod	BDNF,null
R123	T325	T312	auxpass	is,reduced
R1230	T2098	T2099	amod	null,mice
R1231	T2099	T2096	pobj	mice,in
R1232	T2100	T2099	amod	mutant,mice
R1233	T2101	T2088	punct	", ",is
R1234	T2102	T2088	acomp	apparent,is
R1235	T2103	T2088	prep	in,is
R1236	T2104	T2105	det	the,saccule
R1237	T2105	T2103	pobj	saccule,in
R1238	T2106	T2105	cc	or,saccule
R1239	T2107	T2105	conj	utricle,saccule
R124	T326	T312	advmod	severely,reduced
R1240	T2108	T2105	prep	of,saccule
R1241	T2109	T2110	npadvmod	Brn3c,null
R1242	T2110	T2111	amod	null,mice
R1243	T2111	T2108	pobj	mice,of
R1244	T2112	T2088	punct	.,is
R1245	T2114	T2115	amod	Similar,patterns
R1246	T2115	T2116	nsubjpass	patterns,obtained
R1247	T2117	T2115	prep	of,patterns
R1248	T2118	T2117	pobj	innervation,of
R1249	T2119	T2116	auxpass	are,obtained
R125	T327	T328	preconj	both,qualitatively
R1250	T2120	T2116	advcl	using,obtained
R1251	T2121	T2122	amod	acteylated,tubulin
R1252	T2122	T2123	compound	tubulin,immunocytochemistry
R1253	T2123	T2120	dobj	immunocytochemistry,using
R1254	T2124	T2125	punct	(,2b
R1255	T2125	T2116	parataxis	2b,obtained
R1256	T2126	T2125	compound	Fig.,2b
R1257	T2127	T2125	punct	),2b
R1258	T2128	T2116	punct	.,obtained
R1259	T2131	T2132	advcl	Consistent,show
R126	T328	T312	advmod	qualitatively,reduced
R1260	T2133	T2131	prep	with,Consistent
R1261	T2134	T2135	det	the,finding
R1262	T2135	T2133	pobj	finding,with
R1263	T2136	T2135	prep	of,finding
R1264	T2137	T2136	pobj	Xiang,of
R1265	T2138	T2139	advmod	et,al.
R1266	T2139	T2137	advmod	al.,Xiang
R1267	T2140	T2141	punct	[,3
R1268	T2141	T2137	parataxis	3,Xiang
R1269	T2142	T2141	punct	],3
R127	T329	T328	cc	and,qualitatively
R1270	T2143	T2135	prep	of,finding
R1271	T2144	T2145	advmod	only,loss
R1272	T2145	T2143	pobj	loss,of
R1273	T2146	T2145	det	a,loss
R1274	T2147	T2148	nummod	29,%
R1275	T2148	T2145	compound	%,loss
R1276	T2149	T2145	prep	of,loss
R1277	T2150	T2151	amod	spiral,neurons
R1278	T2151	T2149	pobj	neurons,of
R1279	T2152	T2151	amod	sensory,neurons
R128	T330	T328	conj	quantitatively,qualitatively
R1280	T2153	T2145	prep	at,loss
R1281	T2154	T2153	pobj	P4,at
R1282	T2155	T2132	punct	", ",show
R1283	T2156	T2157	poss	our,data
R1284	T2157	T2132	nsubj	data,show
R1285	T2158	T2159	amod	little,difference
R1286	T2159	T2132	dobj	difference,show
R1287	T2160	T2159	prep	in,difference
R1288	T2161	T2162	det	the,pattern
R1289	T2162	T2160	pobj	pattern,in
R129	T331	T312	punct	.,reduced
R1290	T2163	T2162	prep	of,pattern
R1291	T2164	T2163	pobj	innervation,of
R1292	T2165	T2164	prep	of,innervation
R1293	T2166	T2167	det	the,cochlea
R1294	T2167	T2165	pobj	cochlea,of
R1295	T2168	T2162	prep	in,pattern
R1296	T2169	T2170	nmod	P0,mutants
R1297	T2170	T2168	pobj	mutants,in
R1298	T2171	T2170	nmod	Brn3c,mutants
R1299	T2172	T2170	amod	null,mutants
R13	T200	T201	det	some,innervation
R130	T333	T334	nummod	1,%
R1300	T2173	T2174	punct	(,2d
R1301	T2174	T2132	parataxis	2d,show
R1302	T2175	T2174	nmod	Fig.,2d
R1303	T2176	T2174	nmod	2c,2d
R1304	T2177	T2174	punct	",",2d
R1305	T2178	T2174	punct	),2d
R1306	T2179	T2132	punct	.,show
R1307	T2181	T2182	det	No,loss
R1308	T2182	T2184	nsubjpass	loss,observed
R1309	T2183	T2182	amod	selective,loss
R131	T334	T335	nsubj	%,are
R1310	T2185	T2182	prep	of,loss
R1311	T2186	T2187	amod	spiral,neurons
R1312	T2187	T2185	pobj	neurons,of
R1313	T2188	T2184	auxpass	is,observed
R1314	T2189	T2184	prep	in,observed
R1315	T2190	T2191	nmod	Brn3c,mutants
R1316	T2191	T2189	pobj	mutants,in
R1317	T2192	T2191	amod	null,mutants
R1318	T2193	T2184	prep	in,observed
R1319	T2194	T2195	det	the,turn
R132	T336	T334	prep	of,%
R1320	T2195	T2193	pobj	turn,in
R1321	T2196	T2195	amod	basal,turn
R1322	T2197	T2184	punct	", ",observed
R1323	T2198	T2199	det	a,feature
R1324	T2199	T2184	npadvmod	feature,observed
R1325	T2200	T2199	prep	of,feature
R1326	T2201	T2202	preconj	either,NT
R1327	T2202	T2203	nmod	NT,loss
R1328	T2203	T2200	pobj	loss,of
R1329	T2204	T2202	punct	-,NT
R133	T337	T338	compound	myosin,VIIa
R1330	T2205	T2202	nummod	3,NT
R1331	T2206	T2202	cc	or,NT
R1332	T2207	T2202	conj	trkC,NT
R1333	T2208	T2209	punct	[,14
R1334	T2209	T2184	parataxis	14,observed
R1335	T2210	T2209	nummod	6,14
R1336	T2211	T2209	punct	",",14
R1337	T2212	T2209	nummod	9,14
R1338	T2213	T2209	punct	",",14
R1339	T2214	T2209	punct	],14
R134	T338	T339	npadvmod	VIIa,positive
R1340	T2215	T2184	punct	.,observed
R1341	T2217	T2218	advmod	Likewise,shows
R1342	T2219	T2218	punct	", ",shows
R1343	T2220	T2221	det	the,innervation
R1344	T2221	T2218	nsubj	innervation,shows
R1345	T2222	T2221	prep	of,innervation
R1346	T2223	T2224	det	the,apex
R1347	T2224	T2222	pobj	apex,of
R1348	T2225	T2226	punct	(,2f
R1349	T2226	T2221	parataxis	2f,innervation
R135	T339	T341	amod	positive,cells
R1350	T2227	T2226	compound	Fig.,2f
R1351	T2228	T2226	punct	),2f
R1352	T2229	T2230	det	no,abnormality
R1353	T2230	T2218	dobj	abnormality,shows
R1354	T2231	T2230	amod	detectable,abnormality
R1355	T2232	T2230	prep	in,abnormality
R1356	T2233	T2234	amod	overall,pattern
R1357	T2234	T2232	pobj	pattern,in
R1358	T2235	T2234	prep	of,pattern
R1359	T2236	T2235	pobj	innervation,of
R136	T340	T339	punct	-,positive
R1360	T2237	T2230	prep	compared,abnormality
R1361	T2238	T2237	prep	to,compared
R1362	T2239	T2240	compound	control,animals
R1363	T2240	T2238	pobj	animals,to
R1364	T2241	T2242	punct	(,shown
R1365	T2242	T2230	parataxis	shown,abnormality
R1366	T2243	T2242	nsubj	data,shown
R1367	T2244	T2242	neg	not,shown
R1368	T2245	T2242	punct	),shown
R1369	T2246	T2218	punct	", ",shows
R137	T341	T336	pobj	cells,of
R1370	T2247	T2248	det	an,indication
R1371	T2248	T2218	npadvmod	indication,shows
R1372	T2249	T2250	mark	that,expressed
R1373	T2250	T2248	acl	expressed,indication
R1374	T2251	T2250	nsubjpass	BDNF,expressed
R1375	T2252	T2250	aux	could,expressed
R1376	T2253	T2250	auxpass	be,expressed
R1377	T2254	T2250	prep	in,expressed
R1378	T2255	T2256	det	the,apex
R1379	T2256	T2254	pobj	apex,in
R138	T342	T341	amod	immature,cells
R1380	T2257	T2258	punct	[,6
R1381	T2258	T2218	parataxis	6,shows
R1382	T2259	T2258	punct	],6
R1383	T2260	T2218	punct	.,shows
R1384	T2262	T2263	prep	In,shows
R1385	T2264	T2262	pobj	addition,In
R1386	T2265	T2263	punct	", ",shows
R1387	T2266	T2267	det	the,pattern
R1388	T2267	T2263	nsubj	pattern,shows
R1389	T2268	T2267	prep	of,pattern
R139	T343	T341	compound	hair,cells
R1390	T2269	T2270	amod	efferent,innervation
R1391	T2270	T2268	pobj	innervation,of
R1392	T2271	T2272	det	no,deviation
R1393	T2272	T2263	dobj	deviation,shows
R1394	T2273	T2272	prep	from,deviation
R1395	T2274	T2273	pobj	normal,from
R1396	T2275	T2263	advmod	either,shows
R1397	T2276	T2277	punct	(,2e
R1398	T2277	T2263	parataxis	2e,shows
R1399	T2278	T2277	compound	Fig.,2e
R14	T201	T199	pobj	innervation,of
R140	T344	T335	acomp	present,are
R1400	T2279	T2277	punct	),2e
R1401	T2280	T2263	punct	", ",shows
R1402	T2281	T2282	mark	whereas,show
R1403	T2282	T2263	advcl	show,shows
R1404	T2283	T2282	nsubj	they,show
R1405	T2284	T2285	det	the,pattern
R1406	T2285	T2282	dobj	pattern,show
R1407	T2286	T2285	amod	same,pattern
R1408	T2287	T2285	prep	of,pattern
R1409	T2288	T2287	pobj	loss,of
R141	T345	T335	prep	in,are
R1410	T2289	T2285	prep	as,pattern
R1411	T2290	T2291	amod	afferent,fibers
R1412	T2291	T2289	pobj	fibers,as
R1413	T2292	T2291	prep	in,fibers
R1414	T2293	T2294	nmod	neurotrophin,mutants
R1415	T2294	T2292	pobj	mutants,in
R1416	T2295	T2294	amod	null,mutants
R1417	T2296	T2297	punct	[,16
R1418	T2297	T2282	parataxis	16,show
R1419	T2298	T2297	punct	],16
R142	T346	T347	det	the,cochlea
R1420	T2299	T2263	punct	.,shows
R1421	T2301	T2302	det	These,data
R1422	T2302	T2303	nsubj	data,suggest
R1423	T2304	T2305	mark	that,develop
R1424	T2305	T2303	ccomp	develop,suggest
R1425	T2306	T2307	det	the,neurons
R1426	T2307	T2305	nsubj	neurons,develop
R1427	T2308	T2307	amod	spiral,neurons
R1428	T2309	T2307	amod	sensory,neurons
R1429	T2310	T2311	advmod	qualitatively,normal
R143	T347	T345	pobj	cochlea,in
R1430	T2311	T2305	advcl	normal,develop
R1431	T2312	T2313	advmod	at,least
R1432	T2313	T2314	advmod	least,until
R1433	T2314	T2305	prep	until,develop
R1434	T2315	T2314	pobj	P0,until
R1435	T2316	T2305	cc	and,develop
R1436	T2317	T2318	advmod	therefore,allow
R1437	T2318	T2305	conj	allow,develop
R1438	T2319	T2320	amod	normal,pathfinding
R1439	T2320	T2318	dobj	pathfinding,allow
R144	T348	T347	amod	mutant,cochlea
R1440	T2321	T2320	prep	of,pathfinding
R1441	T2322	T2321	pobj	efferents,of
R1442	T2323	T2303	punct	.,suggest
R1443	T2325	T2326	advmod	Most,interestingly
R1444	T2326	T2327	advmod	interestingly,is
R1445	T2328	T2327	punct	", ",is
R1446	T2329	T2327	expl	there,is
R1447	T2330	T2331	det	no,increase
R1448	T2331	T2327	attr	increase,is
R1449	T2332	T2331	prep	in,increase
R145	T349	T335	punct	", ",are
R1450	T2333	T2334	amod	radial,fiber
R1451	T2334	T2335	compound	fiber,spacing
R1452	T2335	T2332	pobj	spacing,in
R1453	T2336	T2335	prep	in,spacing
R1454	T2337	T2338	det	the,apex
R1455	T2338	T2336	pobj	apex,in
R1456	T2339	T2327	punct	", ",is
R1457	T2340	T2341	det	a,problem
R1458	T2341	T2327	npadvmod	problem,is
R1459	T2342	T2341	amod	specific,problem
R146	T350	T335	advcl	concentrated,are
R1460	T2343	T2341	prep	of,problem
R1461	T2344	T2345	nmod	BDNF,mutants
R1462	T2345	T2343	pobj	mutants,of
R1463	T2346	T2345	amod	null,mutants
R1464	T2347	T2348	punct	[,13
R1465	T2348	T2327	parataxis	13,is
R1466	T2349	T2348	nummod	6,13
R1467	T2350	T2348	punct	",",13
R1468	T2351	T2348	punct	],13
R1469	T2352	T2327	punct	.,is
R147	T351	T350	prep	in,concentrated
R1470	T2354	T2355	advmod	However,is
R1471	T2356	T2355	punct	", ",is
R1472	T2357	T2355	expl	there,is
R1473	T2358	T2359	nummod	one,difference
R1474	T2359	T2355	attr	difference,is
R1475	T2360	T2359	amod	qualitative,difference
R1476	T2361	T2362	neg	not,recognized
R1477	T2362	T2359	acl	recognized,difference
R1478	T2363	T2362	prep	in,recognized
R1479	T2364	T2365	det	any,mutant
R148	T352	T353	det	the,base
R1480	T2365	T2363	pobj	mutant,in
R1481	T2366	T2367	amod	single,neurotrophin
R1482	T2367	T2368	npadvmod	neurotrophin,null
R1483	T2368	T2365	amod	null,mutant
R1484	T2369	T2355	punct	.,is
R1485	T2371	T2372	nsubj	Afferents,reach
R1486	T2373	T2374	det	all,rows
R1487	T2374	T2372	dobj	rows,reach
R1488	T2375	T2374	nummod	three,rows
R1489	T2376	T2374	prep	of,rows
R149	T353	T351	pobj	base,in
R1490	T2377	T2378	amod	outer,cells
R1491	T2378	T2376	pobj	cells,of
R1492	T2379	T2378	compound	hair,cells
R1493	T2380	T2372	prep	in,reach
R1494	T2381	T2382	det	the,turn
R1495	T2382	T2380	pobj	turn,in
R1496	T2383	T2382	amod	basal,turn
R1497	T2384	T2382	prep	of,turn
R1498	T2385	T2386	nmod	control,littermates
R1499	T2386	T2384	pobj	littermates,of
R15	T202	T201	amod	afferent,innervation
R150	T354	T335	punct	.,are
R1500	T2387	T2386	amod	wildtype,littermates
R1501	T2388	T2389	punct	(,2c
R1502	T2389	T2372	parataxis	2c,reach
R1503	T2390	T2389	compound	Fig.,2c
R1504	T2391	T2389	punct	),2c
R1505	T2392	T2372	punct	", ",reach
R1506	T2393	T2372	cc	but,reach
R1507	T2394	T2395	preconj	both,afferent
R1508	T2395	T2396	amod	afferent,outgrowth
R1509	T2396	T2399	nsubj	outgrowth,is
R151	T356	T357	quantmod	Around,20
R1510	T2397	T2395	cc	and,afferent
R1511	T2398	T2395	conj	efferent,afferent
R1512	T2399	T2372	conj	is,reach
R1513	T2400	T2399	acomp	disorganized,is
R1514	T2401	T2400	prep	to,disorganized
R1515	T2402	T2403	amod	outer,cells
R1516	T2403	T2401	pobj	cells,to
R1517	T2404	T2403	compound	hair,cells
R1518	T2405	T2399	prep	in,is
R1519	T2406	T2407	npadvmod	Brn3c,null
R152	T357	T358	nummod	20,%
R1520	T2407	T2408	amod	null,mutants
R1521	T2408	T2405	pobj	mutants,in
R1522	T2409	T2410	punct	(,2d
R1523	T2410	T2399	parataxis	2d,is
R1524	T2411	T2410	compound	Fig.,2d
R1525	T2412	T2410	punct	),2d
R1526	T2413	T2399	cc	and,is
R1527	T2414	T2415	aux	does,show
R1528	T2415	T2399	conj	show,is
R1529	T2416	T2415	neg	not,show
R153	T358	T359	nsubj	%,exist
R1530	T2417	T2418	det	any,organization
R1531	T2418	T2415	dobj	organization,show
R1532	T2419	T2418	amod	clear,organization
R1533	T2420	T2418	prep	into,organization
R1534	T2421	T2422	nummod	three,bundles
R1535	T2422	T2420	pobj	bundles,into
R1536	T2423	T2422	amod	distinct,bundles
R1537	T2424	T2422	amod	longitudinal,bundles
R1538	T2425	T2422	compound	fiber,bundles
R1539	T2426	T2422	acl	paralleling,bundles
R154	T360	T358	prep	of,%
R1540	T2427	T2428	det	the,rows
R1541	T2428	T2426	dobj	rows,paralleling
R1542	T2429	T2428	nummod	three,rows
R1543	T2430	T2428	prep	in,rows
R1544	T2431	T2432	det	the,region
R1545	T2432	T2430	pobj	region,in
R1546	T2433	T2432	amod	outer,region
R1547	T2434	T2435	compound	hair,cell
R1548	T2435	T2432	compound	cell,region
R1549	T2436	T2399	punct	.,is
R155	T361	T362	amod	immature,cells
R1550	T2438	T2439	det	These,data
R1551	T2439	T2440	nsubj	data,suggest
R1552	T2441	T2442	mark	that,disrupted
R1553	T2442	T2440	ccomp	disrupted,suggest
R1554	T2443	T2444	compound	fiber,organization
R1555	T2444	T2442	nsubjpass	organization,disrupted
R1556	T2445	T2444	prep	in,organization
R1557	T2446	T2447	det	the,region
R1558	T2447	T2445	pobj	region,in
R1559	T2448	T2447	amod	outer,region
R156	T362	T360	pobj	cells,of
R1560	T2449	T2450	compound	hair,cell
R1561	T2450	T2447	compound	cell,region
R1562	T2451	T2442	auxpass	is,disrupted
R1563	T2452	T2442	advmod	partly,disrupted
R1564	T2453	T2442	prep	in,disrupted
R1565	T2454	T2455	npadvmod	Brn3c,null
R1566	T2455	T2456	amod	null,mutant
R1567	T2456	T2453	pobj	mutant,in
R1568	T2457	T2440	punct	.,suggest
R157	T363	T362	compound	hair,cells
R158	T364	T359	prep	in,exist
R159	T365	T366	det	the,epithelia
R16	T203	T204	amod	inner,ear
R160	T366	T364	pobj	epithelia,in
R161	T367	T366	amod	mutant,epithelia
R162	T368	T366	amod	vestibular,epithelia
R163	T369	T366	amod	sensory,epithelia
R164	T370	T359	punct	.,exist
R165	T372	T373	prep	Despite,retains
R166	T374	T375	advmod	more,severe
R167	T375	T376	amod	severe,loss
R168	T376	T372	pobj	loss,Despite
R169	T377	T376	prep	of,loss
R17	T204	T201	compound	ear,innervation
R170	T378	T379	compound	hair,cells
R171	T379	T377	pobj	cells,of
R172	T380	T381	punct	(,%
R173	T381	T376	parataxis	%,loss
R174	T382	T381	nummod	1,%
R175	T383	T381	prep	compared,%
R176	T384	T383	prep	to,compared
R177	T385	T386	nummod	20,%
R178	T386	T384	pobj	%,to
R179	T387	T381	punct	),%
R18	T209	T210	nsubj	Ears,develop
R180	T388	T373	punct	", ",retains
R181	T389	T390	det	the,cochlea
R182	T390	T373	nsubj	cochlea,retains
R183	T391	T392	amod	many,neurons
R184	T392	T373	dobj	neurons,retains
R185	T393	T392	amod	more,neurons
R186	T394	T392	amod	sensory,neurons
R187	T395	T396	punct	(,%
R188	T396	T392	parataxis	%,neurons
R189	T397	T396	nummod	46,%
R19	T211	T209	prep	of,Ears
R190	T398	T396	prep	compared,%
R191	T399	T398	prep	to,compared
R192	T400	T401	nummod	15,%
R193	T401	T399	pobj	%,to
R194	T402	T396	punct	),%
R195	T403	T392	prep	than,neurons
R196	T404	T405	amod	vestibular,epithelia
R197	T405	T403	pobj	epithelia,than
R198	T406	T373	punct	.,retains
R199	T408	T409	advmod	Even,mice
R20	T212	T213	nmod	Brn3c,mutants
R200	T409	T414	nsubj	mice,have
R201	T410	T411	nummod	6,months
R202	T411	T412	npadvmod	months,old
R203	T412	T409	amod	old,mice
R204	T413	T409	amod	mutant,mice
R205	T415	T416	det	some,fibers
R206	T416	T414	dobj	fibers,have
R207	T417	T416	prep	to,fibers
R208	T418	T419	det	all,epithelia
R209	T419	T417	pobj	epithelia,to
R21	T213	T211	pobj	mutants,of
R210	T420	T419	amod	vestibular,epithelia
R211	T421	T419	amod	sensory,epithelia
R212	T422	T416	cc	and,fibers
R213	T423	T424	amod	many,more
R214	T424	T416	conj	more,fibers
R215	T425	T424	prep	to,more
R216	T426	T427	det	the,apex
R217	T427	T425	pobj	apex,to
R218	T428	T427	amod	cochlear,apex
R219	T429	T430	dep	which,lacks
R22	T214	T213	amod	null,mutants
R220	T430	T427	relcl	lacks,apex
R221	T431	T432	npadvmod	MyoVIIa,positive
R222	T432	T433	amod	positive,cells
R223	T433	T430	dobj	cells,lacks
R224	T434	T433	compound	hair,cells
R225	T435	T414	punct	.,have
R226	T437	T438	advmod	Topologically,organized
R227	T438	T439	amod	organized,projections
R228	T439	T442	nsubj	projections,exist
R229	T440	T441	amod	central,cochlea
R23	T215	T216	amod	immature,cells
R230	T441	T439	compound	cochlea,projections
R231	T443	T444	advmod	at,least
R232	T444	T445	advmod	least,until
R233	T445	T442	prep	until,exist
R234	T446	T445	pobj	P8,until
R235	T447	T442	punct	", ",exist
R236	T448	T442	advcl	suggesting,exist
R237	T449	T450	mark	that,required
R238	T450	T448	ccomp	required,suggesting
R239	T451	T452	amod	functional,cells
R24	T216	T210	dobj	cells,develop
R240	T452	T450	nsubjpass	cells,required
R241	T453	T452	compound	hair,cells
R242	T454	T450	auxpass	are,required
R243	T455	T450	neg	not,required
R244	T456	T457	aux	to,establish
R245	T457	T450	advcl	establish,required
R246	T458	T459	amod	such,projections
R247	T459	T457	dobj	projections,establish
R248	T460	T442	punct	.,exist
R249	T464	T465	det	The,expression
R25	T217	T216	compound	hair,cells
R250	T465	T467	nsubj	expression,suffices
R251	T466	T465	amod	limited,expression
R252	T468	T465	prep	of,expression
R253	T469	T468	pobj	neurotrophins,of
R254	T470	T465	prep	in,expression
R255	T471	T472	det	the,cochlea
R256	T472	T470	pobj	cochlea,in
R257	T473	T472	prep	of,cochlea
R258	T474	T475	npadvmod	Brn3c,null
R259	T475	T476	amod	null,mice
R26	T218	T216	punct	", ",cells
R260	T476	T473	pobj	mice,of
R261	T477	T478	aux	to,support
R262	T478	T467	xcomp	support,suffices
R263	T479	T480	amod	many,neurons
R264	T480	T478	dobj	neurons,support
R265	T481	T480	amod	sensory,neurons
R266	T482	T478	punct	", ",support
R267	T483	T484	advmod	particularly,in
R268	T484	T478	prep	in,support
R269	T485	T486	det	the,cochlea
R27	T219	T216	amod	identifiable,cells
R270	T486	T484	pobj	cochlea,in
R271	T487	T478	punct	", ",support
R272	T488	T478	prep	until,support
R273	T489	T488	pobj	birth,until
R274	T490	T467	punct	.,suffices
R275	T492	T493	det	The,nature
R276	T493	T495	nsubj	nature,remains
R277	T494	T493	amod	molecular,nature
R278	T496	T493	prep	of,nature
R279	T497	T498	det	the,survival
R28	T220	T221	advmod	only,by
R280	T498	T496	pobj	survival,of
R281	T499	T500	amod	long,term
R282	T500	T498	compound	term,survival
R283	T501	T498	prep	of,survival
R284	T502	T503	amod	apical,spiral
R285	T503	T504	nmod	spiral,neurons
R286	T504	T501	pobj	neurons,of
R287	T505	T495	oprd	unclear,remains
R288	T506	T495	punct	.,remains
R29	T221	T219	prep	by,identifiable
R30	T222	T223	amod	certain,markers
R300	T820	T821	nsubj	Brn3c,is
R301	T822	T823	det	a,factor
R302	T823	T821	attr	factor,is
R303	T824	T825	compound	POU,domain
R304	T825	T823	compound	domain,factor
R305	T826	T827	dep	that,is
R306	T827	T823	relcl	is,factor
R307	T828	T827	acomp	crucial,is
R308	T829	T828	prep	for,crucial
R309	T830	T831	amod	inner,ear
R31	T223	T221	pobj	markers,by
R310	T831	T832	nmod	ear,development
R311	T832	T829	pobj	development,for
R312	T833	T834	compound	hair,cell
R313	T834	T832	compound	cell,development
R314	T835	T821	punct	.,is
R315	T837	T838	amod	Targeted,mutants
R316	T838	T841	nsubj	mutants,have
R317	T839	T838	amod	null,mutants
R318	T840	T838	compound	Brn3c,mutants
R319	T842	T843	det	no,cells
R32	T224	T223	amod	molecular,markers
R320	T843	T841	dobj	cells,have
R321	T844	T843	amod	mature,cells
R322	T845	T843	compound	hair,cells
R323	T846	T847	punct	[,2
R324	T847	T841	parataxis	2,have
R325	T848	T847	nummod	1,2
R326	T849	T847	punct	",",2
R327	T850	T847	punct	],2
R328	T851	T841	punct	.,have
R329	T853	T854	compound	Close,examination
R33	T225	T210	punct	", ",develop
R330	T854	T855	nsubj	examination,revealed
R331	T856	T855	aux	has,revealed
R332	T857	T858	mark	that,form
R333	T858	T855	ccomp	form,revealed
R334	T859	T860	det	some,cells
R335	T860	T858	nsubj	cells,form
R336	T861	T860	punct	',cells
R337	T862	T860	amod	immature,cells
R338	T863	T860	punct	',cells
R339	T864	T860	compound	hair,cells
R34	T226	T210	cc	and,develop
R340	T865	T858	prep	in,form
R341	T866	T867	nmod	Brn3c,mutants
R342	T867	T865	pobj	mutants,in
R343	T868	T867	amod	null,mutants
R344	T869	T858	cc	and,form
R345	T870	T858	conj	express,form
R346	T871	T872	amod	cellular,markers
R347	T872	T870	dobj	markers,express
R348	T873	T874	amod	such,as
R349	T874	T872	prep	as,markers
R35	T227	T210	conj	undergo,develop
R350	T875	T876	compound	Myosin,VI
R351	T876	T874	pobj	VI,as
R352	T877	T876	cc	and,VI
R353	T878	T876	conj	VIIa,VI
R354	T879	T876	punct	", ",VI
R355	T880	T876	conj	calretinin,VI
R356	T881	T880	cc	and,calretinin
R357	T882	T880	conj	parvalbumin,calretinin
R358	T883	T884	punct	[,3
R359	T884	T855	parataxis	3,revealed
R36	T228	T227	dobj	apoptosis,undergo
R360	T885	T884	punct	],3
R361	T886	T855	punct	.,revealed
R362	T888	T889	advmod	Furthermore,undergo
R363	T890	T889	punct	", ",undergo
R364	T891	T892	det	these,cells
R365	T892	T889	nsubj	cells,undergo
R366	T893	T892	amod	immature,cells
R367	T894	T892	compound	hair,cells
R368	T895	T892	prep	of,cells
R369	T896	T897	nmod	Brn3c,mutants
R37	T229	T227	prep	in,undergo
R370	T897	T895	pobj	mutants,of
R371	T898	T897	amod	null,mutants
R372	T899	T889	dobj	apoptosis,undergo
R373	T900	T889	prep	in,undergo
R374	T901	T900	pobj	neonates,in
R375	T902	T903	punct	[,3
R376	T903	T889	parataxis	3,undergo
R377	T904	T903	punct	],3
R378	T905	T889	punct	.,undergo
R379	T907	T908	advcl	Consistent,suggested
R38	T230	T229	pobj	neonates,in
R380	T909	T907	prep	with,Consistent
R381	T910	T911	det	an,absence
R382	T911	T909	pobj	absence,with
R383	T912	T911	amod	apparent,absence
R384	T913	T911	prep	of,absence
R385	T914	T915	amod	mature,cells
R386	T915	T913	pobj	cells,of
R387	T916	T915	compound	hair,cells
R388	T917	T908	punct	", ",suggested
R389	T918	T919	amod	initial,work
R39	T231	T210	punct	.,develop
R390	T919	T908	nsubj	work,suggested
R391	T920	T921	mark	that,lost
R392	T921	T908	ccomp	lost,suggested
R393	T922	T923	det	all,vestibular
R394	T923	T921	nsubjpass	vestibular,lost
R395	T924	T923	cc	and,vestibular
R396	T925	T926	det	most,spiral
R397	T926	T927	nmod	spiral,cells
R398	T927	T923	conj	cells,vestibular
R399	T928	T927	compound	ganglion,cells
R4	T190	T192	nsubj	mice,show
R40	T233	T234	det	This,development
R400	T929	T921	auxpass	are,lost
R401	T930	T921	prep	by,lost
R402	T931	T932	amod	postnatal,day
R403	T932	T930	pobj	day,by
R404	T933	T932	nummod	14,day
R405	T934	T932	punct	(,day
R406	T935	T932	appos	P14,day
R407	T936	T921	punct	;,lost
R408	T937	T938	punct	[,1
R409	T938	T921	parataxis	1,lost
R41	T234	T236	nsubj	development,lead
R410	T939	T938	punct	],1
R411	T940	T921	punct	),lost
R412	T941	T908	punct	.,suggested
R413	T943	T944	advmod	However,reported
R414	T945	T944	punct	", ",reported
R415	T946	T947	advmod	more,quantification
R416	T947	T944	nsubj	quantification,reported
R417	T948	T947	amod	detailed,quantification
R418	T949	T947	prep	by,quantification
R419	T950	T949	pobj	others,by
R42	T235	T234	amod	partial,development
R420	T951	T952	punct	[,3
R421	T952	T947	parataxis	3,quantification
R422	T953	T952	punct	],3
R423	T954	T955	mark	that,lost
R424	T955	T944	ccomp	lost,reported
R425	T956	T955	prep	at,lost
R426	T957	T956	pobj	P4,at
R427	T958	T959	quantmod	about,77
R428	T959	T960	nummod	77,%
R429	T960	T955	nsubjpass	%,lost
R43	T237	T234	prep	of,development
R430	T961	T960	prep	of,%
R431	T962	T963	amod	vestibular,neurons
R432	T963	T961	pobj	neurons,of
R433	T964	T960	cc	and,%
R434	T965	T966	advmod	only,29
R435	T966	T967	nummod	29,%
R436	T967	T960	conj	%,%
R437	T968	T967	prep	of,%
R438	T969	T970	amod	spiral,neurons
R439	T970	T968	pobj	neurons,of
R44	T238	T239	compound	hair,cells
R440	T971	T955	auxpass	are,lost
R441	T972	T944	punct	.,reported
R442	T974	T975	nsubjpass	It,suggested
R443	T976	T975	auxpass	was,suggested
R444	T977	T978	mark	that,is
R445	T978	T975	ccomp	is,suggested
R446	T979	T978	expl	there,is
R447	T980	T978	advmod	possibly,is
R448	T981	T982	det	a,loss
R449	T982	T978	attr	loss,is
R45	T239	T237	pobj	cells,of
R450	T983	T982	amod	complete,loss
R451	T984	T978	prep	in,is
R452	T985	T984	pobj	adults,in
R453	T986	T987	punct	[,3
R454	T987	T975	parataxis	3,suggested
R455	T988	T987	punct	],3
R456	T989	T975	punct	.,suggested
R457	T991	T992	advmod	Other,exists
R458	T993	T991	prep	than,Other
R459	T994	T995	det	these,statements
R46	T240	T236	aux	could,lead
R460	T995	T993	pobj	statements,than
R461	T996	T995	amod	preliminary,statements
R462	T997	T992	punct	", ",exists
R463	T998	T999	det	no,data
R464	T999	T992	nsubj	data,exists
R465	T1000	T992	prep	concerning,exists
R466	T1001	T1002	det	the,pattern
R467	T1002	T1000	pobj	pattern,concerning
R468	T1003	T1002	amod	detailed,pattern
R469	T1004	T1002	prep	of,pattern
R47	T241	T236	prep	to,lead
R470	T1005	T1004	pobj	loss,of
R471	T1006	T1005	prep	of,loss
R472	T1007	T1006	pobj	innervation,of
R473	T1008	T1005	prep	in,loss
R474	T1009	T1010	npadvmod	Brn3c,null
R475	T1010	T1011	amod	null,mutants
R476	T1011	T1008	pobj	mutants,in
R477	T1012	T992	punct	.,exists
R478	T1014	T1015	det	The,development
R479	T1015	T1017	nsubj	development,lead
R48	T242	T243	amod	enough,expression
R480	T1016	T1015	amod	initial,development
R481	T1018	T1015	cc	and,development
R482	T1019	T1020	amod	partial,differentiation
R483	T1020	T1015	conj	differentiation,development
R484	T1021	T1015	prep	of,development
R485	T1022	T1023	compound	hair,cells
R486	T1023	T1021	pobj	cells,of
R487	T1024	T1015	prep	in,development
R488	T1025	T1026	compound	Brn3c,mutants
R489	T1026	T1024	pobj	mutants,in
R49	T243	T241	pobj	expression,to
R490	T1027	T1017	aux	could,lead
R491	T1028	T1017	advmod	possibly,lead
R492	T1029	T1017	prep	to,lead
R493	T1030	T1031	det	some,expression
R494	T1031	T1029	pobj	expression,to
R495	T1032	T1031	compound	neurotrophin,expression
R496	T1033	T1031	prep	in,expression
R497	T1034	T1035	det	these,cells
R498	T1035	T1033	pobj	cells,in
R499	T1036	T1037	aux	to,sustain
R5	T191	T190	amod	mutant,mice
R50	T244	T243	compound	neurotrophin,expression
R500	T1037	T1017	advcl	sustain,lead
R501	T1038	T1039	amod	sensory,neurons
R502	T1039	T1037	dobj	neurons,sustain
R503	T1040	T1037	prep	through,sustain
R504	T1041	T1042	amod	embryonic,development
R505	T1042	T1040	pobj	development,through
R506	T1043	T1040	cc	and,through
R507	T1044	T1040	conj	beyond,through
R508	T1045	T1017	punct	.,lead
R509	T1047	T1048	nsubj	Data,shown
R51	T245	T246	aux	to,sustain
R510	T1049	T1047	prep	on,Data
R511	T1050	T1051	amod	various,mutants
R512	T1051	T1049	pobj	mutants,on
R513	T1052	T1053	amod	single,neurotrophin
R514	T1053	T1056	npadvmod	neurotrophin,null
R515	T1054	T1052	cc	and,single
R516	T1055	T1052	conj	compound,single
R517	T1056	T1051	amod	null,mutants
R518	T1057	T1048	aux	have,shown
R519	T1058	T1059	mark	that,leads
R52	T246	T243	acl	sustain,expression
R520	T1059	T1048	ccomp	leads,shown
R521	T1060	T1061	det	the,loss
R522	T1061	T1059	nsubj	loss,leads
R523	T1062	T1061	prep	of,loss
R524	T1063	T1064	det	a,neurotrophin
R525	T1064	T1062	pobj	neurotrophin,of
R526	T1065	T1064	amod	specific,neurotrophin
R527	T1066	T1059	prep	to,leads
R528	T1067	T1068	advmod	topologically,restricted
R529	T1068	T1069	amod	restricted,loss
R53	T247	T248	amod	sensory,neurons
R530	T1069	T1066	pobj	loss,to
R531	T1070	T1069	prep	of,loss
R532	T1071	T1072	amod	sensory,neurons
R533	T1072	T1070	pobj	neurons,of
R534	T1073	T1069	prep	in,loss
R535	T1074	T1075	det	the,ear
R536	T1075	T1073	pobj	ear,in
R537	T1076	T1075	amod	embryonic,ear
R538	T1077	T1078	punct	[,5
R539	T1078	T1048	parataxis	5,shown
R54	T248	T246	dobj	neurons,sustain
R540	T1079	T1078	nummod	4,5
R541	T1080	T1078	punct	",",5
R542	T1081	T1078	punct	],5
R543	T1082	T1048	punct	.,shown
R544	T1084	T1085	amod	Such,loss
R545	T1085	T1087	nsubj	loss,indicate
R546	T1086	T1085	amod	selective,loss
R547	T1088	T1085	prep	in,loss
R548	T1089	T1090	nmod	Brn3c,mutants
R549	T1090	T1088	pobj	mutants,in
R55	T249	T246	prep	through,sustain
R550	T1091	T1090	amod	null,mutants
R551	T1092	T1087	aux	would,indicate
R552	T1093	T1087	advmod	therefore,indicate
R553	T1094	T1087	dobj	reduction,indicate
R554	T1095	T1094	prep	of,reduction
R555	T1096	T1097	det	a,neurotrophin
R556	T1097	T1095	pobj	neurotrophin,of
R557	T1098	T1097	amod	specific,neurotrophin
R558	T1099	T1094	prep	in,reduction
R559	T1100	T1101	amod	immature,cells
R56	T250	T251	amod	embryonic,development
R560	T1101	T1099	pobj	cells,in
R5602	T8591	T8592	compound	Size,variations
R5603	T8593	T8592	prep	of,variations
R5604	T8594	T8595	amod	vestibular,ganglia
R5605	T8595	T8593	pobj	ganglia,of
R5606	T8596	T8592	prep	in,variations
R5607	T8597	T8598	nmod	control,littermates
R5608	T8598	T8596	pobj	littermates,in
R5609	T8599	T8597	cc	and,control
R561	T1102	T1101	compound	hair,cells
R5610	T8600	T8597	conj	mutant,control
R5611	T8601	T8592	acl	labeled,variations
R5612	T8602	T8601	prep	with,labeled
R5613	T8603	T8602	pobj	DiI,with
R5614	T8604	T8592	punct	.,variations
R5615	T8606	T8607	prep	In,shows
R5616	T8608	T8609	amod	newborn,animals
R5617	T8609	T8606	pobj	animals,In
R5618	T8610	T8607	punct	", ",shows
R5619	T8611	T8612	det	the,ganglion
R562	T1103	T1087	punct	.,indicate
R5620	T8612	T8607	nsubj	ganglion,shows
R5621	T8613	T8612	amod	vestibular,ganglion
R5622	T8614	T8615	det	a,reduction
R5623	T8615	T8607	dobj	reduction,shows
R5624	T8616	T8615	amod	dramatic,reduction
R5625	T8617	T8615	prep	in,reduction
R5626	T8618	T8619	npadvmod	Brn3c,null
R5627	T8619	T8620	amod	null,mutants
R5628	T8620	T8617	pobj	mutants,in
R5629	T8621	T8622	punct	(,b
R563	T1105	T1106	advmod	Moreover,shows
R5630	T8622	T8620	parataxis	b,mutants
R5631	T8623	T8622	punct	),b
R5632	T8624	T8615	prep	compared,reduction
R5633	T8625	T8624	prep	to,compared
R5634	T8626	T8627	compound	control,littermates
R5635	T8627	T8625	pobj	littermates,to
R5636	T8628	T8629	punct	(,a
R5637	T8629	T8627	parataxis	a,littermates
R5638	T8630	T8629	punct	),a
R5639	T8631	T8607	punct	.,shows
R564	T1107	T1106	punct	", ",shows
R5640	T8633	T8634	dep	Abbreviations,AC
R5641	T8635	T8633	prep	for,Abbreviations
R5642	T8636	T8635	pobj	this,for
R5643	T8637	T8636	cc	and,this
R5644	T8638	T8639	nmod	other,figures
R5645	T8639	T8636	conj	figures,this
R5646	T8640	T8634	punct	: ,AC
R5647	T8641	T8634	punct	", ",AC
R5648	T8642	T8643	amod	anterior,crista
R5649	T8643	T8634	appos	crista,AC
R565	T1108	T1109	amod	recent,work
R5650	T8644	T8634	punct	;,AC
R5651	T8645	T8634	appos	ggl,AC
R5652	T8646	T8645	punct	", ",ggl
R5653	T8647	T8645	appos	ganglion,ggl
R5654	T8648	T8634	punct	;,AC
R5655	T8649	T8634	appos	c,AC
R5656	T8650	T8649	punct	", ",c
R5657	T8651	T8652	amod	spiral,capillary
R5658	T8652	T8649	appos	capillary,c
R5659	T8653	T8634	punct	;,AC
R566	T1109	T1106	nsubj	work,shows
R5660	T8654	T8655	compound	Genic.,ggl.
R5661	T8655	T8634	appos	ggl.,AC
R5662	T8656	T8655	punct	", ",ggl.
R5663	T8657	T8658	amod	geniculate,ganglion
R5664	T8658	T8655	appos	ganglion,ggl.
R5665	T8659	T8660	punct	;,GER
R5666	T8660	T8634	appos	GER,AC
R5667	T8661	T8660	punct	", ",GER
R5668	T8662	T8663	amod	greater,ridge
R5669	T8663	T8660	appos	ridge,GER
R567	T1110	T1111	mark	that,expressed
R5670	T8664	T8663	amod	epithelial,ridge
R5671	T8665	T8634	punct	;,AC
R5672	T8666	T8634	appos	HC,AC
R5673	T8667	T8666	punct	", ",HC
R5674	T8668	T8669	amod	horizontal,crista
R5675	T8669	T8666	appos	crista,HC
R5676	T8670	T8634	punct	;,AC
R5677	T8671	T8634	appos	HaC,AC
R5678	T8672	T8671	punct	", ",HaC
R5679	T8673	T8674	amod	vestibular,cell
R568	T1111	T1106	ccomp	expressed,shows
R5680	T8674	T8671	appos	cell,HaC
R5681	T8675	T8674	compound	hair,cell
R5682	T8676	T8634	punct	;,AC
R5683	T8677	T8634	appos	IHC,AC
R5684	T8678	T8677	punct	", ",IHC
R5685	T8679	T8680	amod	inner,cell
R5686	T8680	T8677	appos	cell,IHC
R5687	T8681	T8680	compound	hair,cell
R5688	T8682	T8634	punct	;,AC
R5689	T8683	T8634	appos	iHC,AC
R569	T1112	T1111	prep	in,expressed
R5690	T8684	T8683	punct	", ",iHC
R5691	T8685	T8686	amod	immature,cell
R5692	T8686	T8683	appos	cell,iHC
R5693	T8687	T8686	compound	hair,cell
R5694	T8688	T8634	punct	;,AC
R5695	T8689	T8634	appos	IGSB,AC
R5696	T8690	T8689	punct	", ",IGSB
R5697	T8691	T8692	amod	intraganglionic,bundle
R5698	T8692	T8689	appos	bundle,IGSB
R5699	T8693	T8692	amod	spiral,bundle
R57	T251	T249	pobj	development,through
R570	T1113	T1112	pobj	embryos,in
R5700	T8694	T8634	punct	;,AC
R5701	T8695	T8634	appos	OHC,AC
R5702	T8696	T8695	punct	", ",OHC
R5703	T8697	T8698	amod	outer,cell
R5704	T8698	T8695	appos	cell,OHC
R5705	T8699	T8698	compound	hair,cell
R5706	T8700	T8634	punct	;,AC
R5707	T8701	T8634	appos	PC,AC
R5708	T8702	T8701	punct	", ",PC
R5709	T8703	T8704	amod	posterior,crista
R571	T1114	T1111	nsubjpass	NT,expressed
R5710	T8704	T8701	appos	crista,PC
R5711	T8705	T8634	punct	;,AC
R5712	T8706	T8634	appos	S,AC
R5713	T8707	T8706	punct	", ",S
R5714	T8708	T8706	appos	saccule,S
R5715	T8709	T8634	punct	;,AC
R5716	T8710	T8634	appos	SG,AC
R5717	T8711	T8710	punct	", ",SG
R5718	T8712	T8713	amod	spiral,ganglion
R5719	T8713	T8710	appos	ganglion,SG
R572	T1115	T1114	punct	-,NT
R5720	T8714	T8634	punct	;,AC
R5721	T8715	T8634	appos	TM,AC
R5722	T8716	T8715	punct	", ",TM
R5723	T8717	T8718	amod	tectorial,membrane
R5724	T8718	T8715	appos	membrane,TM
R5725	T8719	T8634	punct	;,AC
R5726	T8720	T8634	appos	U,AC
R5727	T8721	T8720	punct	", ",U
R5728	T8722	T8720	appos	utricle,U
R5729	T8723	T8634	punct	;,AC
R573	T1116	T1114	nummod	3,NT
R5730	T8724	T8634	appos	VCN,AC
R5731	T8725	T8724	punct	", ",VCN
R5732	T8726	T8727	amod	ventral,nucleus
R5733	T8727	T8724	appos	nucleus,VCN
R5734	T8728	T8727	amod	cochlear,nucleus
R5735	T8729	T8634	punct	;,AC
R5736	T8730	T8634	appos	VG,AC
R5737	T8731	T8730	punct	", ",VG
R5738	T8732	T8733	amod	vestibular,ganglion
R5739	T8733	T8730	appos	ganglion,VG
R574	T1117	T1111	auxpass	is,expressed
R5740	T8734	T8634	punct	.,AC
R5741	T8736	T8737	nsubj	Bar,indicates
R5742	T8738	T8739	nummod	1000,μm
R5743	T8739	T8737	dobj	μm,indicates
R5744	T8740	T8737	punct	.,indicates
R5745	T8782	T8783	nsubjpass	Innervation,shown
R5746	T8784	T8782	prep	of,Innervation
R5747	T8785	T8786	nmod	Brn3c,ears
R5748	T8786	T8784	pobj	ears,of
R5749	T8787	T8788	amod	null,control
R575	T1118	T1111	advmod	primarily,expressed
R5750	T8788	T8786	compound	control,ears
R5751	T8789	T8788	cc	and,control
R5752	T8790	T8783	auxpass	are,shown
R5753	T8791	T8783	prep	for,shown
R5754	T8792	T8793	amod	newborn,mice
R5755	T8793	T8791	pobj	mice,for
R5756	T8794	T8783	punct	.,shown
R5757	T8796	T8797	expl	There,is
R5758	T8798	T8799	det	no,loss
R5759	T8799	T8797	attr	loss,is
R576	T1119	T1111	prep	in,expressed
R5760	T8800	T8799	amod	specific,loss
R5761	T8801	T8799	prep	of,loss
R5762	T8802	T8801	pobj	fibers,of
R5763	T8803	T8799	prep	to,loss
R5764	T8804	T8805	det	any,endorgan
R5765	T8805	T8803	pobj	endorgan,to
R5766	T8806	T8805	amod	vestibular,endorgan
R5767	T8807	T8797	punct	", ",is
R5768	T8808	T8809	mark	as,visualized
R5769	T8809	T8797	advcl	visualized,is
R577	T1120	T1121	amod	supporting,cells
R5770	T8810	T8809	agent	by,visualized
R5771	T8811	T8812	compound	DiI,labeling
R5772	T8812	T8810	pobj	labeling,by
R5773	T8813	T8814	punct	(,a
R5774	T8814	T8812	parataxis	a,labeling
R5775	T8815	T8814	punct	),a
R5776	T8816	T8812	cc	or,labeling
R5777	T8817	T8818	amod	acetylated,tubulin
R5778	T8818	T8819	compound	tubulin,immunoreactivity
R5779	T8819	T8812	conj	immunoreactivity,labeling
R578	T1121	T1119	pobj	cells,in
R5780	T8820	T8821	punct	(,b
R5781	T8821	T8797	parataxis	b,is
R5782	T8822	T8821	punct	),b
R5783	T8823	T8797	punct	.,is
R5784	T8825	T8826	det	No,differences
R5785	T8826	T8828	nsubjpass	differences,found
R5786	T8827	T8826	amod	major,differences
R5787	T8829	T8826	prep	in,differences
R5788	T8830	T8829	pobj	pattern,in
R5789	T8831	T8830	prep	of,pattern
R579	T1122	T1111	punct	", ",expressed
R5790	T8832	T8831	pobj	projection,of
R5791	T8833	T8832	prep	through,projection
R5792	T8834	T8835	compound	radial,fibers
R5793	T8835	T8833	pobj	fibers,through
R5794	T8836	T8828	auxpass	are,found
R5795	T8837	T8828	prep	in,found
R5796	T8838	T8839	det	the,cochlea
R5797	T8839	T8837	pobj	cochlea,in
R5798	T8840	T8839	prep	of,cochlea
R5799	T8841	T8842	nmod	Brn3c,mutants
R58	T252	T236	punct	.,lead
R580	T1123	T1111	advcl	moving,expressed
R5800	T8842	T8840	pobj	mutants,of
R5801	T8843	T8842	amod	null,mutants
R5802	T8844	T8845	punct	(,f
R5803	T8845	T8842	parataxis	f,mutants
R5804	T8846	T8845	nmod	d,f
R5805	T8847	T8845	punct	",",f
R5806	T8848	T8845	punct	),f
R5807	T8849	T8828	prep	as,found
R5808	T8850	T8849	prep	compared,as
R5809	T8851	T8850	prep	to,compared
R581	T1124	T1125	advmod	only,around
R5810	T8852	T8853	compound	control,littermates
R5811	T8853	T8851	pobj	littermates,to
R5812	T8854	T8855	punct	(,c
R5813	T8855	T8853	parataxis	c,littermates
R5814	T8856	T8855	punct	),c
R5815	T8857	T8828	punct	.,found
R5816	T8860	T8859	punct	", ",Note
R5817	T8861	T8859	advmod	however,Note
R5818	T8862	T8859	punct	", ",Note
R5819	T8863	T8864	det	the,lack
R582	T1125	T1123	prep	around,moving
R5820	T8864	T8859	dobj	lack,Note
R5821	T8865	T8864	prep	of,lack
R5822	T8866	T8867	amod	orderly,outgrowth
R5823	T8867	T8865	pobj	outgrowth,of
R5824	T8868	T8867	compound	fiber,outgrowth
R5825	T8869	T8867	prep	to,outgrowth
R5826	T8870	T8871	det	the,cells
R5827	T8871	T8869	pobj	cells,to
R5828	T8872	T8871	amod	outer,cells
R5829	T8873	T8871	compound	hair,cells
R583	T1126	T1125	pobj	birth,around
R5830	T8874	T8875	punct	(,d
R5831	T8875	T8859	parataxis	d,Note
R5832	T8876	T8875	nmod	c,d
R5833	T8877	T8875	punct	", ",d
R5834	T8878	T8875	punct	),d
R5835	T8879	T8859	punct	.,Note
R5836	T8881	T8882	amod	Efferent,fibers
R5837	T8882	T8883	nsubj	fibers,show
R5838	T8884	T8882	prep	to,fibers
R5839	T8885	T8886	det	the,ear
R584	T1127	T1123	prep	into,moving
R5840	T8886	T8884	pobj	ear,to
R5841	T8887	T8888	det	a,bundle
R5842	T8888	T8883	dobj	bundle,show
R5843	T8889	T8890	advmod	well,developed
R5844	T8890	T8888	amod	developed,bundle
R5845	T8891	T8888	amod	intraganglionic,bundle
R5846	T8892	T8888	amod	spiral,bundle
R5847	T8893	T8888	punct	(,bundle
R5848	T8894	T8888	appos	IGSB,bundle
R5849	T8895	T8888	punct	),bundle
R585	T1128	T1129	compound	hair,cells
R5850	T8896	T8888	prep	with,bundle
R5851	T8897	T8898	det	no,differences
R5852	T8898	T8896	pobj	differences,with
R5853	T8899	T8898	amod	detectable,differences
R5854	T8900	T8898	prep	compared,differences
R5855	T8901	T8900	prep	to,compared
R5856	T8902	T8901	pobj	controls,to
R5857	T8903	T8904	punct	(,e
R5858	T8904	T8883	parataxis	e,show
R5859	T8905	T8904	punct	),e
R586	T1129	T1127	pobj	cells,into
R5860	T8906	T8883	punct	.,show
R5861	T8908	T8909	nsubj	Bar,indicates
R5862	T8910	T8911	nummod	100,μm
R5863	T8911	T8909	dobj	μm,indicates
R5864	T8912	T8909	punct	.,indicates
R587	T1130	T1131	punct	[,7
R588	T1131	T1106	parataxis	7,shows
R589	T1132	T1131	nummod	6,7
R59	T254	T255	nsubj	We,investigated
R590	T1133	T1131	punct	",",7
R591	T1134	T1131	punct	],7
R592	T1135	T1106	punct	.,shows
R593	T1137	T1138	prep	In,suggests
R594	T1139	T1137	pobj	fact,In
R595	T1140	T1138	punct	", ",suggests
R596	T1141	T1142	det	the,loss
R597	T1142	T1138	nsubj	loss,suggests
R598	T1143	T1142	amod	selective,loss
R599	T1144	T1142	prep	of,loss
R6	T193	T194	amod	long,term
R60	T256	T255	aux	have,investigated
R600	T1145	T1144	pobj	vestibular,of
R601	T1146	T1145	prep	as,vestibular
R602	T1147	T1146	prep	compared,as
R603	T1148	T1147	prep	to,compared
R604	T1149	T1148	pobj	cochlear,to
R605	T1150	T1151	amod	sensory,neurons
R606	T1151	T1145	appos	neurons,vestibular
R607	T1152	T1153	punct	(,%
R608	T1153	T1145	parataxis	%,vestibular
R609	T1154	T1153	nummod	77,%
R61	T257	T255	advmod	therefore,investigated
R610	T1155	T1153	cc	versus,%
R611	T1156	T1157	nummod	29,%
R612	T1157	T1153	conj	%,%
R613	T1158	T1153	punct	;,%
R614	T1159	T1160	punct	[,3
R615	T1160	T1153	parataxis	3,%
R616	T1161	T1160	punct	],3
R617	T1162	T1153	punct	),%
R618	T1163	T1164	mark	that,be
R619	T1164	T1138	ccomp	be,suggests
R62	T258	T255	prep	in,investigated
R620	T1165	T1166	nmod	NT,expression
R621	T1166	T1164	nsubj	expression,be
R622	T1167	T1165	punct	-,NT
R623	T1168	T1165	nummod	3,NT
R624	T1169	T1164	aux	may,be
R625	T1170	T1171	advmod	less,downregulated
R626	T1171	T1164	acomp	downregulated,be
R627	T1172	T1164	prep	in,be
R628	T1173	T1174	nmod	Brn3c,mutants
R629	T1174	T1172	pobj	mutants,in
R63	T259	T260	det	these,mutants
R630	T1175	T1174	amod	null,mutants
R631	T1176	T1164	prep	than,be
R632	T1177	T1176	pobj	BDNF,than
R633	T1178	T1179	punct	[,9
R634	T1179	T1164	parataxis	9,be
R635	T1180	T1179	nummod	6,9
R636	T1181	T1179	punct	",",9
R637	T1182	T1179	nummod	8,9
R638	T1183	T1179	punct	",",9
R639	T1184	T1179	punct	],9
R64	T260	T258	pobj	mutants,in
R640	T1185	T1164	punct	", ",be
R641	T1186	T1164	prep	provided,be
R642	T1187	T1188	mark	that,takes
R643	T1188	T1186	pcomp	takes,provided
R644	T1189	T1190	advmod	at,least
R645	T1190	T1191	advmod	least,differentiation
R646	T1191	T1188	nsubj	differentiation,takes
R647	T1192	T1191	det	some,differentiation
R648	T1193	T1191	prep	of,differentiation
R649	T1194	T1195	amod	supporting,cells
R65	T261	T262	det	the,patterns
R650	T1195	T1193	pobj	cells,of
R651	T1196	T1188	dobj	place,takes
R652	T1197	T1138	punct	.,suggests
R653	T1199	T1200	prep	In,downregulated
R654	T1201	T1202	det	the,ear
R655	T1202	T1199	pobj	ear,In
R656	T1203	T1204	punct	[,4
R657	T1204	T1199	parataxis	4,In
R658	T1205	T1204	punct	],4
R659	T1206	T1207	advmod	as,as
R66	T262	T255	dobj	patterns,investigated
R660	T1207	T1199	cc	as,In
R661	T1208	T1207	advmod	well,as
R662	T1209	T1210	advmod	elsewhere,10
R663	T1210	T1199	conj	10,In
R664	T1211	T1210	punct	[,10
R665	T1212	T1210	punct	],10
R666	T1213	T1200	nsubjpass	neurotrophins,downregulated
R667	T1214	T1200	auxpass	are,downregulated
R668	T1215	T1200	advmod	progressively,downregulated
R669	T1216	T1200	prep	in,downregulated
R67	T263	T262	prep	of,patterns
R670	T1217	T1218	amod	postnatal,mammals
R671	T1218	T1216	pobj	mammals,in
R672	T1219	T1200	cc	and,downregulated
R673	T1220	T1221	advmod	possibly,replaced
R674	T1221	T1200	conj	replaced,downregulated
R675	T1222	T1221	agent	by,replaced
R676	T1223	T1224	amod	other,factors
R677	T1224	T1222	pobj	factors,by
R678	T1225	T1226	punct	[,11
R679	T1226	T1221	parataxis	11,replaced
R68	T264	T263	pobj	innervation,of
R680	T1227	T1226	punct	],11
R681	T1228	T1200	punct	.,downregulated
R682	T1230	T1231	nsubj	We,investigated
R683	T1232	T1231	aux	have,investigated
R684	T1233	T1231	prep	in,investigated
R685	T1234	T1233	pobj	detail,in
R686	T1235	T1236	det	the,pattern
R687	T1236	T1231	dobj	pattern,investigated
R688	T1237	T1236	prep	of,pattern
R689	T1238	T1237	pobj	innervation,of
R69	T265	T263	cc	and,of
R690	T1239	T1236	prep	in,pattern
R691	T1240	T1241	det	the,mutants
R692	T1241	T1239	pobj	mutants,in
R693	T1242	T1241	compound	Brn3c,mutants
R694	T1243	T1236	punct	", ",pattern
R695	T1244	T1245	advmod	as,as
R696	T1245	T1236	cc	as,pattern
R697	T1246	T1245	advmod	well,as
R698	T1247	T1248	det	the,expression
R699	T1248	T1236	conj	expression,pattern
R7	T194	T196	nmod	term,retention
R70	T266	T263	conj	of,of
R700	T1249	T1248	prep	of,expression
R701	T1250	T1249	pobj	NT,of
R702	T1251	T1250	punct	-,NT
R703	T1252	T1250	nummod	3,NT
R704	T1253	T1250	cc	and,NT
R705	T1254	T1250	conj	BDNF,NT
R706	T1255	T1231	punct	.,investigated
R707	T1257	T1258	nsubj	We,want
R708	T1259	T1260	aux	to,evaluate
R709	T1260	T1258	xcomp	evaluate,want
R71	T267	T266	pobj	expression,of
R710	T1261	T1262	det	a,correlation
R711	T1262	T1260	dobj	correlation,evaluate
R712	T1263	T1262	amod	possible,correlation
R713	T1264	T1262	prep	between,correlation
R714	T1265	T1266	det	the,topology
R715	T1266	T1264	pobj	topology,between
R716	T1267	T1266	prep	of,topology
R717	T1268	T1269	amod	sensory,neuron
R718	T1269	T1270	compound	neuron,loss
R719	T1270	T1267	pobj	loss,of
R72	T268	T266	prep	of,of
R720	T1271	T1266	cc	and,topology
R721	T1272	T1266	conj	absence,topology
R722	T1273	T1272	prep	of,absence
R723	T1274	T1275	det	a,neurotrophin
R724	T1275	T1273	pobj	neurotrophin,of
R725	T1276	T1275	amod	specific,neurotrophin
R726	T1277	T1275	cc	or,neurotrophin
R727	T1278	T1279	amod	topological,loss
R728	T1279	T1275	conj	loss,neurotrophin
R729	T1280	T1279	prep	of,loss
R73	T269	T270	amod	known,neurotrophins
R730	T1281	T1282	compound	hair,cells
R731	T1282	T1280	pobj	cells,of
R732	T1283	T1262	prep	at,correlation
R733	T1284	T1283	pobj	birth,at
R734	T1285	T1283	cc	and,at
R735	T1286	T1283	conj	in,at
R736	T1287	T1288	amod	older,animals
R737	T1288	T1286	pobj	animals,in
R738	T1289	T1258	punct	.,want
R739	T1291	T1292	det	This,information
R74	T270	T268	pobj	neurotrophins,of
R740	T1292	T1293	nsubj	information,be
R741	T1294	T1293	aux	could,be
R742	T1295	T1293	acomp	important,be
R743	T1296	T1295	prep	for,important
R744	T1297	T1298	det	an,evaluation
R745	T1298	T1296	pobj	evaluation,for
R746	T1299	T1298	nmod	in,evaluation
R747	T1300	T1299	punct	-,in
R748	T1301	T1299	pobj	depth,in
R749	T1302	T1298	prep	of,evaluation
R75	T271	T255	punct	.,investigated
R750	T1303	T1304	det	the,deafness
R751	T1304	T1302	pobj	deafness,of
R752	T1305	T1304	amod	human,deafness
R753	T1306	T1304	acl	related,deafness
R754	T1307	T1306	prep	to,related
R755	T1308	T1309	det	the,gene
R756	T1309	T1307	pobj	gene,to
R757	T1310	T1309	compound	Pou4f3,gene
R758	T1311	T1309	punct	", ",gene
R759	T1312	T1309	appos	DFNA15,gene
R76	T275	T276	prep	At,is
R760	T1313	T1314	punct	[,12
R761	T1314	T1293	parataxis	12,be
R762	T1315	T1314	punct	],12
R763	T1316	T1293	punct	.,be
R764	T1318	T1319	nsubj	We,report
R765	T1320	T1319	advmod	here,report
R766	T1321	T1322	amod	long,term
R767	T1322	T1323	compound	term,retention
R768	T1323	T1319	dobj	retention,report
R769	T1324	T1323	prep	of,retention
R77	T277	T275	pobj	birth,At
R770	T1325	T1326	amod	cochlear,neurons
R771	T1326	T1324	pobj	neurons,of
R772	T1327	T1326	amod	sensory,neurons
R773	T1328	T1323	prep	for,retention
R774	T1329	T1330	advmod	at,6
R775	T1330	T1332	nummod	6,months
R776	T1331	T1330	advmod	least,6
R777	T1332	T1328	pobj	months,for
R778	T1333	T1323	punct	", ",retention
R779	T1334	T1335	prep	in,in
R78	T278	T276	expl	there,is
R780	T1335	T1323	prep	in,retention
R781	T1336	T1334	amod	particular,in
R782	T1337	T1338	det	the,apex
R783	T1338	T1335	pobj	apex,in
R784	T1339	T1338	amod	cochlear,apex
R785	T1340	T1323	punct	", ",retention
R786	T1341	T1323	prep	in,retention
R787	T1342	T1343	npadvmod	Brn3c,null
R788	T1343	T1344	amod	null,mice
R789	T1344	T1341	pobj	mice,in
R79	T279	T280	det	a,expression
R790	T1345	T1344	amod	mutant,mice
R791	T1346	T1319	punct	.,report
R792	T1348	T1349	det	This,retention
R793	T1349	T1350	nsubj	retention,is
R794	T1351	T1349	prep	of,retention
R795	T1352	T1351	pobj	afferents,of
R796	T1353	T1352	cc	and,afferents
R797	T1354	T1352	conj	efferents,afferents
R798	T1355	T1350	acomp	unrelated,is
R799	T1356	T1355	prep	to,unrelated
R8	T195	T194	punct	-,term
R80	T280	T276	attr	expression,is
R800	T1357	T1358	compound	hair,cell
R801	T1358	T1359	compound	cell,differentiation
R802	T1359	T1356	pobj	differentiation,to
R803	T1360	T1361	mark	as,detected
R804	T1361	T1350	advcl	detected,is
R805	T1362	T1363	neg	not,cells
R806	T1363	T1361	nsubjpass	cells,detected
R807	T1364	T1363	advmod	even,cells
R808	T1365	T1363	amod	immature,cells
R809	T1366	T1363	compound	hair,cells
R81	T281	T280	amod	limited,expression
R810	T1367	T1361	aux	can,detected
R811	T1368	T1361	auxpass	be,detected
R812	T1369	T1361	prep	at,detected
R813	T1370	T1371	amod	early,stages
R814	T1371	T1369	pobj	stages,at
R815	T1372	T1371	amod	postnatal,stages
R816	T1373	T1361	prep	with,detected
R817	T1374	T1375	compound	MyoVII,immunocytochemistry
R818	T1375	T1373	pobj	immunocytochemistry,with
R819	T1376	T1361	prep	in,detected
R82	T282	T280	prep	of,expression
R820	T1377	T1378	det	this,part
R821	T1378	T1376	pobj	part,in
R822	T1379	T1378	prep	of,part
R823	T1380	T1381	det	the,cochlea
R824	T1381	T1379	pobj	cochlea,of
R825	T1382	T1350	punct	.,is
R826	T1384	T1385	det	This,retention
R827	T1385	T1386	nsubj	retention,is
R828	T1387	T1385	prep	of,retention
R829	T1388	T1389	amod	apical,neurons
R83	T283	T282	pobj	BDNF,of
R830	T1389	T1387	pobj	neurons,of
R831	T1390	T1389	amod	spiral,neurons
R832	T1391	T1386	advmod	also,is
R833	T1392	T1393	advmod	largely,unrelated
R834	T1393	T1386	acomp	unrelated,is
R835	T1394	T1393	prep	to,unrelated
R836	T1395	T1394	pobj	neurotrophins,to
R837	T1396	T1397	dep	which,known
R838	T1397	T1395	relcl	known,neurotrophins
R839	T1398	T1397	auxpass	are,known
R84	T284	T283	cc	and,BDNF
R840	T1399	T1400	aux	to,reduced
R841	T1400	T1397	xcomp	reduced,known
R842	T1401	T1400	auxpass	be,reduced
R843	T1402	T1400	prep	in,reduced
R844	T1403	T1404	poss	their,expression
R845	T1404	T1402	pobj	expression,in
R846	T1405	T1397	prep	in,known
R847	T1406	T1407	amod	neonatal,rodents
R848	T1407	T1405	pobj	rodents,in
R849	T1408	T1409	punct	[,4
R85	T285	T283	conj	NT,BDNF
R850	T1409	T1386	parataxis	4,is
R851	T1410	T1409	punct	],4
R852	T1411	T1386	punct	.,is
R854	T1476	T1477	aux	To,appreciate
R855	T1477	T1478	advcl	appreciate,want
R856	T1479	T1480	det	the,effects
R857	T1480	T1477	dobj	effects,appreciate
R858	T1481	T1480	prep	of,effects
R859	T1482	T1483	det	the,mutation
R86	T286	T285	punct	-,NT
R860	T1483	T1481	pobj	mutation,of
R861	T1484	T1483	nmod	Brn3c,mutation
R862	T1485	T1483	amod	null,mutation
R863	T1486	T1480	prep	on,effects
R864	T1487	T1488	det	the,pattern
R865	T1488	T1486	pobj	pattern,on
R866	T1489	T1488	prep	of,pattern
R867	T1490	T1491	det	the,innervation
R868	T1491	T1489	pobj	innervation,of
R869	T1492	T1493	amod	inner,ear
R87	T287	T285	nummod	3,NT
R870	T1493	T1491	compound	ear,innervation
R871	T1494	T1478	punct	", ",want
R872	T1495	T1478	nsubj	we,want
R873	T1496	T1478	advmod	first,want
R874	T1497	T1498	aux	to,present
R875	T1498	T1478	xcomp	present,want
R876	T1499	T1500	det	the,effects
R877	T1500	T1498	dobj	effects,present
R878	T1501	T1500	prep	of,effects
R879	T1502	T1503	nmod	BDNF,mutations
R88	T288	T276	prep	in,is
R880	T1503	T1501	pobj	mutations,of
R881	T1504	T1502	cc	and,BDNF
R882	T1505	T1502	conj	NT,BDNF
R883	T1506	T1505	punct	-,NT
R884	T1507	T1505	nummod	3,NT
R885	T1508	T1503	amod	null,mutations
R886	T1509	T1503	prep	at,mutations
R887	T1510	T1509	pobj	birth,at
R888	T1511	T1512	punct	[,14
R889	T1512	T1478	parataxis	14,want
R89	T289	T290	det	the,epithelia
R890	T1513	T1512	nummod	6,14
R891	T1514	T1512	punct	",",14
R892	T1515	T1512	nummod	13,14
R893	T1516	T1512	punct	",",14
R894	T1517	T1512	punct	],14
R895	T1518	T1478	punct	.,want
R896	T1520	T1521	amod	Null,mutants
R897	T1521	T1522	nsubj	mutants,lose
R898	T1523	T1521	prep	of,mutants
R899	T1524	T1523	pobj	BDNF,of
R9	T196	T192	dobj	retention,show
R90	T290	T288	pobj	epithelia,in
R900	T1525	T1524	cc	or,BDNF
R901	T1526	T1527	poss	its,receptor
R902	T1527	T1524	conj	receptor,BDNF
R903	T1528	T1527	appos	trkB,receptor
R904	T1529	T1530	det	all,innervation
R905	T1530	T1522	dobj	innervation,lose
R906	T1531	T1530	prep	to,innervation
R907	T1532	T1533	det	the,canals
R908	T1533	T1531	pobj	canals,to
R909	T1534	T1533	amod	semicircular,canals
R91	T291	T290	amod	mutant,epithelia
R910	T1535	T1522	cc	and,lose
R911	T1536	T1522	conj	have,lose
R912	T1537	T1538	det	a,innervation
R913	T1538	T1536	dobj	innervation,have
R914	T1539	T1538	amod	reduced,innervation
R915	T1540	T1538	prep	to,innervation
R916	T1541	T1542	det	the,utricle
R917	T1542	T1540	pobj	utricle,to
R918	T1543	T1542	punct	", ",utricle
R919	T1544	T1542	conj	saccule,utricle
R92	T292	T290	amod	sensory,epithelia
R920	T1545	T1544	cc	and,saccule
R921	T1546	T1547	amod	apical,turn
R922	T1547	T1544	conj	turn,saccule
R923	T1548	T1547	prep	of,turn
R924	T1549	T1550	det	the,cochlea
R925	T1550	T1548	pobj	cochlea,of
R926	T1551	T1522	punct	.,lose
R927	T1553	T1554	prep	In,result
R928	T1555	T1553	pobj	contrast,In
R929	T1556	T1554	punct	", ",result
R93	T293	T276	cc	and,is
R930	T1557	T1558	amod	null,mutations
R931	T1558	T1554	nsubj	mutations,result
R932	T1559	T1558	prep	of,mutations
R933	T1560	T1561	preconj	either,NT
R934	T1561	T1559	pobj	NT,of
R935	T1562	T1561	punct	-,NT
R936	T1563	T1561	nummod	3,NT
R937	T1564	T1561	cc	or,NT
R938	T1565	T1566	poss	its,receptor
R939	T1566	T1561	conj	receptor,NT
R94	T294	T295	compound	DiI,tracing
R940	T1567	T1566	appos	trkC,receptor
R941	T1568	T1554	prep	in,result
R942	T1569	T1568	pobj	loss,in
R943	T1570	T1569	prep	of,loss
R944	T1571	T1572	amod	spiral,neurons
R945	T1572	T1570	pobj	neurons,of
R946	T1573	T1569	prep	in,loss
R947	T1574	T1575	det	the,turn
R948	T1575	T1573	pobj	turn,in
R949	T1576	T1575	amod	basal,turn
R95	T295	T296	nsubj	tracing,shows
R950	T1577	T1569	prep	with,loss
R951	T1578	T1577	pobj	formation,with
R952	T1579	T1578	prep	of,formation
R953	T1580	T1581	det	an,bundle
R954	T1581	T1579	pobj	bundle,of
R955	T1582	T1583	amod	inner,spiral
R956	T1583	T1581	nmod	spiral,bundle
R957	T1584	T1581	prep	of,bundle
R958	T1585	T1584	pobj	afferents,of
R959	T1586	T1581	acl	extending,bundle
R96	T296	T276	conj	shows,is
R960	T1587	T1586	prep	to,extending
R961	T1588	T1589	det	the,tip
R962	T1589	T1587	pobj	tip,to
R963	T1590	T1589	amod	basal,tip
R964	T1591	T1554	punct	.,result
R965	T1593	T1594	poss	Our,hypothesis
R966	T1594	T1596	nsubj	hypothesis,be
R967	T1595	T1594	amod	null,hypothesis
R968	T1597	T1594	prep	for,hypothesis
R969	T1598	T1599	det	this,study
R97	T297	T298	det	no,reduction
R970	T1599	T1597	pobj	study,for
R971	T1600	T1596	aux	would,be
R972	T1601	T1602	mark	that,show
R973	T1602	T1596	ccomp	show,be
R974	T1603	T1604	nmod	Brn3c,mice
R975	T1604	T1602	nsubj	mice,show
R976	T1605	T1604	amod	null,mice
R977	T1606	T1607	amod	severe,production
R978	T1607	T1602	dobj	production,show
R979	T1608	T1607	amod	compromised,production
R98	T298	T296	dobj	reduction,shows
R980	T1609	T1607	prep	of,production
R981	T1610	T1611	det	these,neurotrophins
R982	T1611	T1609	pobj	neurotrophins,of
R983	T1612	T1602	cc	and,show
R984	T1613	T1614	aux	should,show
R985	T1614	T1602	conj	show,show
R986	T1615	T1614	advmod	therefore,show
R987	T1616	T1617	det	a,pattern
R988	T1617	T1614	dobj	pattern,show
R989	T1618	T1617	amod	comparable,pattern
R99	T299	T298	amod	specific,reduction
R990	T1619	T1617	prep	of,pattern
R991	T1620	T1621	compound	nerve,fiber
R992	T1621	T1622	compound	fiber,loss
R993	T1622	T1619	pobj	loss,of
R994	T1623	T1596	punct	.,be

PMC:149366 / 11-9929 JSONTXT 4 Projects

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PMC:149366 / 11-9929 JSON TXT 4 Projects