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PMC:149350 / 8489-9704 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T2289 0-8 JJ denotes Confocal
T2290 9-19 NN denotes microscopy
T2291 19-144 sentence denotes Lenses were vibratome sectioned into 100–200 μm thick sections along the optical (anterior to posterior) or equatorial axes.
T2292 20-26 NNS denotes Lenses
T2294 27-31 VBD denotes were
T2295 32-41 NN denotes vibratome
T2293 42-51 VBN denotes sectioned
T2296 52-56 IN denotes into
T2297 57-60 CD denotes 100
T2299 60-61 SYM denotes –
T2298 61-64 CD denotes 200
T2300 65-67 NN denotes μm
T2301 68-73 JJ denotes thick
T2302 74-82 NNS denotes sections
T2303 83-88 IN denotes along
T2304 89-92 DT denotes the
T2306 93-100 JJ denotes optical
T2307 101-102 -LRB- denotes (
T2308 102-110 JJ denotes anterior
T2309 111-113 IN denotes to
T2310 114-123 JJ denotes posterior
T2311 123-124 -RRB- denotes )
T2312 125-127 CC denotes or
T2313 128-138 JJ denotes equatorial
T2305 139-143 NNS denotes axes
T2314 143-144 . denotes .
T2315 144-370 sentence denotes Thick sections were fixed in 2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer pH 7.2 at RT for 24 hrs then washed several times in Tris buffered saline (0.5 M Tris, 150 mM NaCl, pH 7.4).
T2316 145-150 JJ denotes Thick
T2317 151-159 NNS denotes sections
T2319 160-164 VBD denotes were
T2318 165-170 VBN denotes fixed
T2320 171-173 IN denotes in
T2321 174-175 CD denotes 2
T2322 175-176 NN denotes %
T2324 177-178 -LRB- denotes (
T2325 178-179 NN denotes w
T2326 179-180 SYM denotes /
T2327 180-181 NN denotes v
T2328 181-182 -RRB- denotes )
T2323 183-199 NN denotes paraformaldehyde
T2329 200-203 CC denotes and
T2330 204-205 CD denotes 2
T2331 205-206 NN denotes %
T2332 207-221 NN denotes glutaraldehyde
T2333 222-223 -LRB- denotes (
T2334 223-224 NN denotes v
T2335 224-225 SYM denotes /
T2336 225-226 NN denotes v
T2337 226-227 -RRB- denotes )
T2338 228-230 IN denotes in
T2339 231-234 CD denotes 0.1
T2340 235-236 NN denotes M
T2342 237-243 NN denotes sodium
T2343 244-254 NN denotes cacodylate
T2341 255-261 NN denotes buffer
T2344 262-264 NN denotes pH
T2345 265-268 CD denotes 7.2
T2346 269-271 IN denotes at
T2347 272-274 NN denotes RT
T2348 275-278 IN denotes for
T2349 279-281 CD denotes 24
T2350 282-285 NNS denotes hrs
T2351 286-290 RB denotes then
T2352 291-297 VBD denotes washed
T2353 298-305 JJ denotes several
T2354 306-311 NNS denotes times
T2355 312-314 IN denotes in
T2356 315-319 NNP denotes Tris
T2357 320-328 VBN denotes buffered
T2358 329-335 NN denotes saline
T2359 336-337 -LRB- denotes (
T2361 337-340 CD denotes 0.5
T2362 341-342 NN denotes M
T2363 343-347 NNP denotes Tris
T2364 347-349 , denotes ,
T2365 349-352 CD denotes 150
T2366 353-355 NN denotes mM
T2367 356-360 NN denotes NaCl
T2368 360-362 , denotes ,
T2360 362-364 NN denotes pH
T2369 365-368 CD denotes 7.4
T2370 368-369 -RRB- denotes )
T2371 369-370 . denotes .
T2372 370-557 sentence denotes To visualize lipid membranes, sections were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI';DiIC18; Molecular Probes) as previously described [24].
T2373 371-373 TO denotes To
T2374 374-383 VB denotes visualize
T2376 384-389 NN denotes lipid
T2377 390-399 NNS denotes membranes
T2378 399-401 , denotes ,
T2379 401-409 NNS denotes sections
T2380 410-414 VBD denotes were
T2375 415-422 VBN denotes stained
T2381 423-427 IN denotes with
T2382 428-429 CD denotes 1
T2384 429-430 , denotes ,
T2385 430-431 CD denotes 1
T2386 431-432 SYM denotes '
T2387 432-433 HYPH denotes -
T2383 433-444 NN denotes dioctadecyl
T2388 444-445 HYPH denotes -
T2389 445-446 CD denotes 3
T2390 446-447 , denotes ,
T2391 447-448 CD denotes 3
T2393 448-449 , denotes ,
T2394 449-450 CD denotes 3
T2395 450-451 SYM denotes '
T2396 451-452 , denotes ,
T2397 452-453 CD denotes 3
T2398 453-454 SYM denotes '
T2399 454-455 HYPH denotes -
T2400 455-482 JJ denotes tetramethylindocarbocyanine
T2392 483-494 NN denotes perchlorate
T2401 495-496 -LRB- denotes (
T2403 496-497 `` denotes '
T2404 497-500 NN denotes DiI
T2405 500-501 '' denotes '
T2406 501-502 : denotes ;
T2407 502-508 NN denotes DiIC18
T2408 508-509 : denotes ;
T2409 510-519 NNP denotes Molecular
T2402 520-526 NNP denotes Probes
T2410 526-527 -RRB- denotes )
T2411 528-530 IN denotes as
T2413 531-541 RB denotes previously
T2412 542-551 VBN denotes described
T2414 552-553 -LRB- denotes [
T2415 553-555 CD denotes 24
T2416 555-556 -RRB- denotes ]
T2417 556-557 . denotes .
T2418 557-812 sentence denotes Sections were then washed several times with Tris buffered saline, and nucleic acids were stained by incubating sections in TBS containing 1 μM SYTOX Green (Molecular Probes) for 10 min at RT followed by several washes with TBS prior to confocal imaging.
T2419 558-566 NNS denotes Sections
T2421 567-571 VBD denotes were
T2422 572-576 RB denotes then
T2420 577-583 VBN denotes washed
T2423 584-591 JJ denotes several
T2424 592-597 NNS denotes times
T2425 598-602 IN denotes with
T2426 603-607 NNP denotes Tris
T2427 608-616 VBN denotes buffered
T2428 617-623 NN denotes saline
T2429 623-625 , denotes ,
T2430 625-628 CC denotes and
T2431 629-636 JJ denotes nucleic
T2432 637-642 NNS denotes acids
T2434 643-647 VBD denotes were
T2433 648-655 VBN denotes stained
T2435 656-658 IN denotes by
T2436 659-669 VBG denotes incubating
T2437 670-678 NNS denotes sections
T2438 679-681 IN denotes in
T2439 682-685 NN denotes TBS
T2440 686-696 VBG denotes containing
T2441 697-698 CD denotes 1
T2442 699-701 NN denotes μM
T2444 702-707 NN denotes SYTOX
T2443 708-713 NN denotes Green
T2445 714-715 -LRB- denotes (
T2447 715-724 JJ denotes Molecular
T2446 725-731 NNP denotes Probes
T2448 731-732 -RRB- denotes )
T2449 733-736 IN denotes for
T2450 737-739 CD denotes 10
T2451 740-743 NN denotes min
T2452 744-746 IN denotes at
T2453 747-749 NN denotes RT
T2454 750-758 VBN denotes followed
T2455 759-761 IN denotes by
T2456 762-769 JJ denotes several
T2457 770-776 NNS denotes washes
T2458 777-781 IN denotes with
T2459 782-785 NN denotes TBS
T2460 786-791 RB denotes prior
T2461 792-794 IN denotes to
T2462 795-803 JJ denotes confocal
T2463 804-811 NN denotes imaging
T2464 811-812 . denotes .
T2465 812-1215 sentence denotes Lens sections were viewed on a Zeiss laser scanning confocal microscope model LSM 410 equipped with an Axiovert 100 inverted microscope, an Argon-Krypton 488/568/647 laser, a KP line selection filter, a FT 488/568 Dichroic beam splitter, a FT 560 Dichroic beam splitter, a LP 590 emission filter for viewing DiI, a BP 515–540 emission filter for viewing SYTOX green, and the software package LSM 3.993.
T2466 813-817 NNP denotes Lens
T2467 818-826 NNS denotes sections
T2469 827-831 VBD denotes were
T2468 832-838 VBN denotes viewed
T2470 839-841 IN denotes on
T2471 842-843 DT denotes a
T2473 844-849 NNP denotes Zeiss
T2474 850-855 NN denotes laser
T2475 856-864 NN denotes scanning
T2476 865-873 JJ denotes confocal
T2472 874-884 NN denotes microscope
T2477 885-890 NN denotes model
T2478 891-894 NN denotes LSM
T2479 895-898 CD denotes 410
T2480 899-907 VBN denotes equipped
T2481 908-912 IN denotes with
T2482 913-915 DT denotes an
T2484 916-924 JJ denotes Axiovert
T2485 925-928 CD denotes 100
T2486 929-937 JJ denotes inverted
T2483 938-948 NN denotes microscope
T2487 948-950 , denotes ,
T2488 950-952 DT denotes an
T2490 953-958 JJ denotes Argon
T2492 958-959 HYPH denotes -
T2491 959-966 JJ denotes Krypton
T2493 967-970 CD denotes 488
T2494 970-971 HYPH denotes /
T2495 971-974 CD denotes 568
T2496 974-975 HYPH denotes /
T2497 975-978 CD denotes 647
T2489 979-984 NN denotes laser
T2498 984-986 , denotes ,
T2499 986-987 DT denotes a
T2501 988-990 NN denotes KP
T2502 991-995 NN denotes line
T2503 996-1005 NN denotes selection
T2500 1006-1012 NN denotes filter
T2504 1012-1014 , denotes ,
T2505 1014-1015 DT denotes a
T2507 1016-1018 NN denotes FT
T2508 1019-1022 CD denotes 488
T2509 1022-1023 HYPH denotes /
T2510 1023-1026 CD denotes 568
T2511 1027-1035 JJ denotes Dichroic
T2512 1036-1040 NN denotes beam
T2506 1041-1049 NN denotes splitter
T2513 1049-1051 , denotes ,
T2514 1051-1052 DT denotes a
T2516 1053-1055 NN denotes FT
T2517 1056-1059 CD denotes 560
T2518 1060-1068 JJ denotes Dichroic
T2519 1069-1073 NN denotes beam
T2515 1074-1082 NN denotes splitter
T2520 1082-1084 , denotes ,
T2521 1084-1085 DT denotes a
T2523 1086-1088 NN denotes LP
T2524 1089-1092 CD denotes 590
T2525 1093-1101 NN denotes emission
T2522 1102-1108 NN denotes filter
T2526 1109-1112 IN denotes for
T2527 1113-1120 VBG denotes viewing
T2528 1121-1124 NNP denotes DiI
T2529 1124-1126 , denotes ,
T2530 1126-1127 DT denotes a
T2532 1128-1130 NN denotes BP
T2533 1131-1134 CD denotes 515
T2534 1134-1135 SYM denotes –
T2535 1135-1138 CD denotes 540
T2536 1139-1147 NN denotes emission
T2531 1148-1154 NN denotes filter
T2537 1155-1158 IN denotes for
T2538 1159-1166 VBG denotes viewing
T2539 1167-1172 NN denotes SYTOX
T2540 1173-1178 NN denotes green
T2541 1178-1180 , denotes ,
T2542 1180-1183 CC denotes and
T2543 1184-1187 DT denotes the
T2545 1188-1196 NN denotes software
T2544 1197-1204 NN denotes package
T2546 1205-1208 NN denotes LSM
T2547 1209-1214 CD denotes 3.993
T2548 1214-1215 . denotes .
R1538 T2289 T2290 amod Confocal,microscopy
R1539 T2292 T2293 nsubjpass Lenses,sectioned
R1540 T2294 T2293 auxpass were,sectioned
R1541 T2295 T2293 dep vibratome,sectioned
R1542 T2296 T2293 prep into,sectioned
R1543 T2297 T2298 quantmod 100,200
R1544 T2298 T2300 nummod 200,μm
R1545 T2299 T2298 punct –,200
R1546 T2300 T2301 npadvmod μm,thick
R1547 T2301 T2302 amod thick,sections
R1548 T2302 T2296 pobj sections,into
R1549 T2303 T2293 prep along,sectioned
R1550 T2304 T2305 det the,axes
R1551 T2305 T2303 pobj axes,along
R1552 T2306 T2305 amod optical,axes
R1553 T2307 T2308 punct (,anterior
R1554 T2308 T2306 parataxis anterior,optical
R1555 T2309 T2308 prep to,anterior
R1556 T2310 T2309 amod posterior,to
R1557 T2311 T2308 punct ),anterior
R1558 T2312 T2306 cc or,optical
R1559 T2313 T2306 conj equatorial,optical
R1560 T2314 T2293 punct .,sectioned
R1561 T2316 T2317 amod Thick,sections
R1562 T2317 T2318 nsubjpass sections,fixed
R1563 T2319 T2318 auxpass were,fixed
R1564 T2320 T2318 prep in,fixed
R1565 T2321 T2322 nummod 2,%
R1566 T2322 T2323 nmod %,paraformaldehyde
R1567 T2323 T2320 pobj paraformaldehyde,in
R1568 T2324 T2325 punct (,w
R1569 T2325 T2322 parataxis w,%
R1570 T2326 T2327 punct /,v
R1571 T2327 T2325 prep v,w
R1572 T2328 T2325 punct ),w
R1573 T2329 T2323 cc and,paraformaldehyde
R1574 T2330 T2331 nummod 2,%
R1575 T2331 T2332 compound %,glutaraldehyde
R1576 T2332 T2323 conj glutaraldehyde,paraformaldehyde
R1577 T2333 T2334 punct (,v
R1578 T2334 T2332 parataxis v,glutaraldehyde
R1579 T2335 T2336 punct /,v
R1580 T2336 T2334 prep v,v
R1581 T2337 T2334 punct ),v
R1582 T2338 T2323 prep in,paraformaldehyde
R1583 T2339 T2340 nummod 0.1,M
R1584 T2340 T2341 compound M,buffer
R1585 T2341 T2338 pobj buffer,in
R1586 T2342 T2343 compound sodium,cacodylate
R1587 T2343 T2341 compound cacodylate,buffer
R1588 T2344 T2323 npadvmod pH,paraformaldehyde
R1589 T2345 T2344 nummod 7.2,pH
R1590 T2346 T2318 prep at,fixed
R1591 T2347 T2346 pobj RT,at
R1592 T2348 T2318 prep for,fixed
R1593 T2349 T2350 nummod 24,hrs
R1594 T2350 T2348 pobj hrs,for
R1595 T2351 T2352 advmod then,washed
R1596 T2352 T2318 dep washed,fixed
R1597 T2353 T2354 amod several,times
R1598 T2354 T2352 npadvmod times,washed
R1599 T2355 T2352 prep in,washed
R1600 T2356 T2357 npadvmod Tris,buffered
R1601 T2357 T2358 amod buffered,saline
R1602 T2358 T2355 pobj saline,in
R1603 T2359 T2360 punct (,pH
R1604 T2360 T2358 parataxis pH,saline
R1605 T2361 T2362 nummod 0.5,M
R1606 T2362 T2363 compound M,Tris
R1607 T2363 T2360 dep Tris,pH
R1608 T2364 T2360 punct ", ",pH
R1609 T2365 T2366 nummod 150,mM
R1610 T2366 T2367 compound mM,NaCl
R1611 T2367 T2360 dep NaCl,pH
R1612 T2368 T2360 punct ", ",pH
R1613 T2369 T2360 nummod 7.4,pH
R1614 T2370 T2360 punct ),pH
R1615 T2371 T2318 punct .,fixed
R1616 T2373 T2374 aux To,visualize
R1617 T2374 T2375 advcl visualize,stained
R1618 T2376 T2377 compound lipid,membranes
R1619 T2377 T2374 dobj membranes,visualize
R1620 T2378 T2375 punct ", ",stained
R1621 T2379 T2375 nsubjpass sections,stained
R1622 T2380 T2375 auxpass were,stained
R1623 T2381 T2375 prep with,stained
R1624 T2382 T2383 nummod 1,dioctadecyl
R1625 T2383 T2381 pobj dioctadecyl,with
R1626 T2384 T2382 punct ",",1
R1627 T2385 T2382 appos 1,1
R1628 T2386 T2385 punct ',1
R1629 T2387 T2383 punct -,dioctadecyl
R1630 T2388 T2383 punct -,dioctadecyl
R1631 T2389 T2383 nummod 3,dioctadecyl
R1632 T2390 T2383 punct ",",dioctadecyl
R1633 T2391 T2392 nummod 3,perchlorate
R1634 T2392 T2383 appos perchlorate,dioctadecyl
R1635 T2393 T2391 punct ",",3
R1636 T2394 T2391 appos 3,3
R1637 T2395 T2394 punct ',3
R1638 T2396 T2391 punct ",",3
R1639 T2397 T2391 appos 3,3
R1640 T2398 T2397 punct ',3
R1641 T2399 T2391 punct -,3
R1642 T2400 T2391 amod tetramethylindocarbocyanine,3
R1643 T2401 T2402 punct (,Probes
R1644 T2402 T2383 parataxis Probes,dioctadecyl
R1645 T2403 T2402 punct ',Probes
R1646 T2404 T2402 dep DiI,Probes
R1647 T2405 T2402 punct ',Probes
R1648 T2406 T2402 punct ;,Probes
R1649 T2407 T2402 dep DiIC18,Probes
R1650 T2408 T2402 punct ;,Probes
R1651 T2409 T2402 compound Molecular,Probes
R1652 T2410 T2402 punct ),Probes
R1653 T2411 T2412 mark as,described
R1654 T2412 T2375 advcl described,stained
R1655 T2413 T2412 advmod previously,described
R1656 T2414 T2415 punct [,24
R1657 T2415 T2375 parataxis 24,stained
R1658 T2416 T2415 punct ],24
R1659 T2417 T2375 punct .,stained
R1660 T2419 T2420 nsubjpass Sections,washed
R1661 T2421 T2420 auxpass were,washed
R1662 T2422 T2420 advmod then,washed
R1663 T2423 T2424 amod several,times
R1664 T2424 T2420 npadvmod times,washed
R1665 T2425 T2420 prep with,washed
R1666 T2426 T2427 npadvmod Tris,buffered
R1667 T2427 T2428 amod buffered,saline
R1668 T2428 T2425 pobj saline,with
R1669 T2429 T2420 punct ", ",washed
R1670 T2430 T2420 cc and,washed
R1671 T2431 T2432 amod nucleic,acids
R1672 T2432 T2433 nsubjpass acids,stained
R1673 T2433 T2420 conj stained,washed
R1674 T2434 T2433 auxpass were,stained
R1675 T2435 T2433 prep by,stained
R1676 T2436 T2435 pcomp incubating,by
R1677 T2437 T2436 dobj sections,incubating
R1678 T2438 T2436 prep in,incubating
R1679 T2439 T2438 pobj TBS,in
R1680 T2440 T2439 acl containing,TBS
R1681 T2441 T2442 nummod 1,μM
R1682 T2442 T2443 compound μM,Green
R1683 T2443 T2440 dobj Green,containing
R1684 T2444 T2443 compound SYTOX,Green
R1685 T2445 T2446 punct (,Probes
R1686 T2446 T2440 parataxis Probes,containing
R1687 T2447 T2446 amod Molecular,Probes
R1688 T2448 T2446 punct ),Probes
R1689 T2449 T2436 prep for,incubating
R1690 T2450 T2451 nummod 10,min
R1691 T2451 T2449 pobj min,for
R1692 T2452 T2436 prep at,incubating
R1693 T2453 T2452 pobj RT,at
R1694 T2454 T2436 advcl followed,incubating
R1695 T2455 T2454 agent by,followed
R1696 T2456 T2457 amod several,washes
R1697 T2457 T2455 pobj washes,by
R1698 T2458 T2457 prep with,washes
R1699 T2459 T2458 pobj TBS,with
R1700 T2460 T2457 advmod prior,washes
R1701 T2461 T2460 prep to,prior
R1702 T2462 T2463 amod confocal,imaging
R1703 T2463 T2461 pobj imaging,to
R1704 T2464 T2433 punct .,stained
R1705 T2466 T2467 compound Lens,sections
R1706 T2467 T2468 nsubjpass sections,viewed
R1707 T2469 T2468 auxpass were,viewed
R1708 T2470 T2468 prep on,viewed
R1709 T2471 T2472 det a,microscope
R1710 T2472 T2470 pobj microscope,on
R1711 T2473 T2474 nmod Zeiss,laser
R1712 T2474 T2472 nmod laser,microscope
R1713 T2475 T2472 nmod scanning,microscope
R1714 T2476 T2472 amod confocal,microscope
R1715 T2477 T2478 compound model,LSM
R1716 T2478 T2472 appos LSM,microscope
R1717 T2479 T2478 nummod 410,LSM
R1718 T2480 T2472 acl equipped,microscope
R1719 T2481 T2480 prep with,equipped
R1720 T2482 T2483 det an,microscope
R1721 T2483 T2481 pobj microscope,with
R1722 T2484 T2485 amod Axiovert,100
R1723 T2485 T2483 nummod 100,microscope
R1724 T2486 T2483 amod inverted,microscope
R1725 T2487 T2483 punct ", ",microscope
R1726 T2488 T2489 det an,laser
R1727 T2489 T2483 conj laser,microscope
R1728 T2490 T2491 amod Argon,Krypton
R1729 T2491 T2489 nmod Krypton,laser
R1730 T2492 T2491 punct -,Krypton
R1731 T2493 T2491 nummod 488,Krypton
R1732 T2494 T2491 punct /,Krypton
R1733 T2495 T2491 nummod 568,Krypton
R1734 T2496 T2491 punct /,Krypton
R1735 T2497 T2491 nummod 647,Krypton
R1736 T2498 T2489 punct ", ",laser
R1737 T2499 T2500 det a,filter
R1738 T2500 T2489 conj filter,laser
R1739 T2501 T2500 compound KP,filter
R1740 T2502 T2503 compound line,selection
R1741 T2503 T2500 compound selection,filter
R1742 T2504 T2500 punct ", ",filter
R1743 T2505 T2506 det a,splitter
R1744 T2506 T2500 conj splitter,filter
R1745 T2507 T2506 nmod FT,splitter
R1746 T2508 T2507 nummod 488,FT
R1747 T2509 T2507 punct /,FT
R1748 T2510 T2507 nummod 568,FT
R1749 T2511 T2506 amod Dichroic,splitter
R1750 T2512 T2506 compound beam,splitter
R1751 T2513 T2506 punct ", ",splitter
R1752 T2514 T2515 det a,splitter
R1753 T2515 T2506 conj splitter,splitter
R1754 T2516 T2515 nmod FT,splitter
R1755 T2517 T2516 nummod 560,FT
R1756 T2518 T2515 amod Dichroic,splitter
R1757 T2519 T2515 compound beam,splitter
R1758 T2520 T2515 punct ", ",splitter
R1759 T2521 T2522 det a,filter
R1760 T2522 T2515 conj filter,splitter
R1761 T2523 T2522 nmod LP,filter
R1762 T2524 T2523 nummod 590,LP
R1763 T2525 T2522 compound emission,filter
R1764 T2526 T2522 prep for,filter
R1765 T2527 T2526 pcomp viewing,for
R1766 T2528 T2527 dobj DiI,viewing
R1767 T2529 T2522 punct ", ",filter
R1768 T2530 T2531 det a,filter
R1769 T2531 T2522 conj filter,filter
R1770 T2532 T2531 nmod BP,filter
R1771 T2533 T2532 nummod 515,BP
R1772 T2534 T2535 punct –,540
R1773 T2535 T2532 prep 540,BP
R1774 T2536 T2531 compound emission,filter
R1775 T2537 T2531 prep for,filter
R1776 T2538 T2537 pcomp viewing,for
R1777 T2539 T2540 compound SYTOX,green
R1778 T2540 T2538 dobj green,viewing
R1779 T2541 T2531 punct ", ",filter
R1780 T2542 T2531 cc and,filter
R1781 T2543 T2544 det the,package
R1782 T2544 T2531 conj package,filter
R1783 T2545 T2544 compound software,package
R1784 T2546 T2544 appos LSM,package
R1785 T2547 T2546 nummod 3.993,LSM
R1786 T2548 T2468 punct .,viewed