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PMC:149350 / 7197-10640 JSONTXT 4 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T1951 9-15 NNS denotes Lenses
T1952 15-87 sentence denotes The wild type mouse strain used in this study was the 129SvEvTac mouse.
T1953 16-19 DT denotes The
T1955 20-24 JJ denotes wild
T1956 25-29 NN denotes type
T1957 30-35 NN denotes mouse
T1954 36-42 NN denotes strain
T1959 43-47 VBN denotes used
T1960 48-50 IN denotes in
T1961 51-55 DT denotes this
T1962 56-61 NN denotes study
T1958 62-65 VBD denotes was
T1963 66-69 DT denotes the
T1965 70-80 NN denotes 129SvEvTac
T1964 81-86 NN denotes mouse
T1966 86-87 . denotes .
T1967 87-169 sentence denotes Lenses examined were from 5(8 lenses), 46(8 lenses) and 72(6 lenses) wk old mice.
T1968 88-94 NNS denotes Lenses
T1970 95-103 VBN denotes examined
T1969 104-108 VBD denotes were
T1971 109-113 IN denotes from
T1972 114-115 CD denotes 5
T1974 115-116 -LRB- denotes (
T1976 116-117 CD denotes 8
T1975 118-124 NNS denotes lenses
T1977 124-125 -RRB- denotes )
T1978 125-127 , denotes ,
T1979 127-129 CD denotes 46
T1980 129-130 -LRB- denotes (
T1982 130-131 CD denotes 8
T1981 132-138 NNS denotes lenses
T1983 138-139 -RRB- denotes )
T1984 140-143 CC denotes and
T1985 144-146 CD denotes 72
T1986 146-147 -LRB- denotes (
T1988 147-148 CD denotes 6
T1987 149-155 NNS denotes lenses
T1989 155-156 -RRB- denotes )
T1973 157-159 NN denotes wk
T1990 160-163 JJ denotes old
T1991 164-168 NNS denotes mice
T1992 168-169 . denotes .
T1993 169-286 sentence denotes AlphaA/BKO was generated by cross breeding alphaAKO-127 [22] and alphaBKO-168 mice [23], also in a 129Sv background.
T1994 170-176 NN denotes AlphaA
T1996 176-177 HYPH denotes /
T1995 177-180 NN denotes BKO
T1998 181-184 VBD denotes was
T1997 185-194 VBN denotes generated
T1999 195-197 IN denotes by
T2000 198-203 NN denotes cross
T2001 204-212 VBG denotes breeding
T2002 213-221 NN denotes alphaAKO
T2004 221-222 HYPH denotes -
T2003 222-225 NN denotes 127
T2006 226-227 -LRB- denotes [
T2007 227-229 CD denotes 22
T2008 229-230 -RRB- denotes ]
T2009 231-234 CC denotes and
T2010 235-243 NN denotes alphaBKO
T2012 243-244 HYPH denotes -
T2011 244-247 NN denotes 168
T2005 248-252 NNS denotes mice
T2013 253-254 -LRB- denotes [
T2014 254-256 CD denotes 23
T2015 256-257 -RRB- denotes ]
T2016 257-259 , denotes ,
T2017 259-263 RB denotes also
T2018 264-266 IN denotes in
T2019 267-268 DT denotes a
T2021 269-274 NN denotes 129Sv
T2020 275-285 NN denotes background
T2022 285-286 . denotes .
T2023 286-336 sentence denotes These mice also lack the HSPB2 gene product [23].
T2024 287-292 DT denotes These
T2025 293-297 NNS denotes mice
T2027 298-302 RB denotes also
T2026 303-307 VBP denotes lack
T2028 308-311 DT denotes the
T2030 312-317 NN denotes HSPB2
T2031 318-322 NN denotes gene
T2029 323-330 NN denotes product
T2032 331-332 -LRB- denotes [
T2033 332-334 CD denotes 23
T2034 334-335 -RRB- denotes ]
T2035 335-336 . denotes .
T2036 336-410 sentence denotes Lenses examined were from 5 wk (6 lenses) and 54 wk (16 lenses) old mice.
T2037 337-343 NNS denotes Lenses
T2039 344-352 VBN denotes examined
T2038 353-357 VBD denotes were
T2040 358-362 IN denotes from
T2041 363-364 CD denotes 5
T2042 365-367 NN denotes wk
T2044 368-369 -LRB- denotes (
T2046 369-370 CD denotes 6
T2045 371-377 NNS denotes lenses
T2047 377-378 -RRB- denotes )
T2048 379-382 CC denotes and
T2049 383-385 CD denotes 54
T2050 386-388 NN denotes wk
T2051 389-390 -LRB- denotes (
T2053 390-392 CD denotes 16
T2052 393-399 NNS denotes lenses
T2054 399-400 -RRB- denotes )
T2043 401-404 JJ denotes old
T2055 405-409 NNS denotes mice
T2056 409-410 . denotes .
T2057 410-579 sentence denotes All animals were treated in accordance with the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research.
T2058 411-414 DT denotes All
T2059 415-422 NNS denotes animals
T2061 423-427 VBD denotes were
T2060 428-435 VBN denotes treated
T2062 436-438 IN denotes in
T2063 439-449 NN denotes accordance
T2064 450-454 IN denotes with
T2065 455-458 DT denotes the
T2067 459-470 NNP denotes Association
T2068 471-474 IN denotes for
T2069 475-483 NNP denotes Research
T2070 484-486 IN denotes in
T2071 487-493 NNP denotes Vision
T2072 494-497 CC denotes and
T2073 498-511 NNP denotes Ophthalmology
T2066 512-521 NNP denotes Statement
T2074 522-525 IN denotes for
T2075 526-529 DT denotes the
T2076 530-533 NNP denotes Use
T2077 534-536 IN denotes of
T2078 537-544 NNPS denotes Animals
T2079 545-547 IN denotes in
T2080 548-558 NNP denotes Ophthalmic
T2082 559-562 CC denotes and
T2083 563-569 NNP denotes Vision
T2081 570-578 NNP denotes Research
T2084 578-579 . denotes .
T2085 579-640 sentence denotes Immediately after euthanizing animals, eyes were enucleated.
T2086 580-591 RB denotes Immediately
T2087 592-597 IN denotes after
T2089 598-609 VBG denotes euthanizing
T2090 610-617 NNS denotes animals
T2091 617-619 , denotes ,
T2092 619-623 NNS denotes eyes
T2093 624-628 VBD denotes were
T2088 629-639 VBN denotes enucleated
T2094 639-640 . denotes .
T2095 640-911 sentence denotes A scalpel blade was then used to make a small incision into the anterior chamber near the equator and then both eyes from individual animals were immersed in 5 ml fixative (2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer, pH 7.2).
T2096 641-642 DT denotes A
T2098 643-650 NN denotes scalpel
T2097 651-656 NN denotes blade
T2100 657-660 VBD denotes was
T2101 661-665 RB denotes then
T2099 666-670 VBN denotes used
T2102 671-673 TO denotes to
T2103 674-678 VB denotes make
T2104 679-680 DT denotes a
T2106 681-686 JJ denotes small
T2105 687-695 NN denotes incision
T2107 696-700 IN denotes into
T2108 701-704 DT denotes the
T2110 705-713 JJ denotes anterior
T2109 714-721 NN denotes chamber
T2111 722-726 IN denotes near
T2112 727-730 DT denotes the
T2113 731-738 NN denotes equator
T2114 739-742 CC denotes and
T2115 743-747 RB denotes then
T2117 748-752 DT denotes both
T2118 753-757 NNS denotes eyes
T2119 758-762 IN denotes from
T2120 763-773 JJ denotes individual
T2121 774-781 NNS denotes animals
T2122 782-786 VBD denotes were
T2116 787-795 VBN denotes immersed
T2123 796-798 IN denotes in
T2124 799-800 CD denotes 5
T2125 801-803 NN denotes ml
T2127 804-812 JJ denotes fixative
T2128 813-814 -LRB- denotes (
T2129 814-815 CD denotes 2
T2130 815-816 NN denotes %
T2131 817-818 -LRB- denotes (
T2132 818-819 NN denotes w
T2133 819-820 SYM denotes /
T2134 820-821 NN denotes v
T2135 821-822 -RRB- denotes )
T2126 823-839 NN denotes paraformaldehyde
T2136 840-843 CC denotes and
T2137 844-845 CD denotes 2
T2138 845-846 NN denotes %
T2139 847-861 NN denotes glutaraldehyde
T2140 862-863 -LRB- denotes (
T2141 863-864 NN denotes v
T2142 864-865 SYM denotes /
T2143 865-866 NN denotes v
T2144 866-867 -RRB- denotes )
T2145 868-870 IN denotes in
T2146 871-874 CD denotes 0.1
T2147 875-876 NN denotes M
T2149 877-883 NN denotes sodium
T2150 884-894 NN denotes cacodylate
T2148 895-901 NN denotes buffer
T2151 901-903 , denotes ,
T2152 903-905 NN denotes pH
T2153 906-909 CD denotes 7.2
T2154 909-910 -RRB- denotes )
T2155 910-911 . denotes .
T2156 911-1006 sentence denotes Eyes were fixed for at least 24 hr at room temperature (RT) prior to dissection of the lenses.
T2157 912-916 NNS denotes Eyes
T2159 917-921 VBD denotes were
T2158 922-927 VBN denotes fixed
T2160 928-931 IN denotes for
T2161 932-934 RB denotes at
T2163 935-940 RBS denotes least
T2162 941-943 CD denotes 24
T2164 944-946 NNS denotes hr
T2165 947-949 IN denotes at
T2166 950-954 NN denotes room
T2167 955-966 NN denotes temperature
T2168 967-968 -LRB- denotes (
T2169 968-970 NN denotes RT
T2170 970-971 -RRB- denotes )
T2171 972-977 JJ denotes prior
T2172 978-980 IN denotes to
T2173 981-991 NN denotes dissection
T2174 992-994 IN denotes of
T2175 995-998 DT denotes the
T2176 999-1005 NNS denotes lenses
T2177 1005-1006 . denotes .
T2178 1006-1109 sentence denotes At this time, equatorial and axial dimensions of lenses and gross lenticular appearance were recorded.
T2179 1007-1009 IN denotes At
T2181 1010-1014 DT denotes this
T2182 1015-1019 NN denotes time
T2183 1019-1021 , denotes ,
T2184 1021-1031 JJ denotes equatorial
T2186 1032-1035 CC denotes and
T2187 1036-1041 JJ denotes axial
T2185 1042-1052 NNS denotes dimensions
T2188 1053-1055 IN denotes of
T2189 1056-1062 NNS denotes lenses
T2190 1063-1066 CC denotes and
T2191 1067-1072 JJ denotes gross
T2193 1073-1083 JJ denotes lenticular
T2192 1084-1094 NN denotes appearance
T2194 1095-1099 VBD denotes were
T2180 1100-1108 VBN denotes recorded
T2195 1108-1109 . denotes .
T2196 1109-1290 sentence denotes Statistical analyses on data consisted of Student's t-test, means and standard deviation, using the statistical software package StatMost (Dataxiom Software Inc., Los Angeles, CA).
T2197 1110-1121 JJ denotes Statistical
T2198 1122-1130 NNS denotes analyses
T2200 1131-1133 IN denotes on
T2201 1134-1138 NNS denotes data
T2199 1139-1148 VBD denotes consisted
T2202 1149-1151 IN denotes of
T2203 1152-1159 NN denotes Student
T2205 1159-1161 POS denotes 's
T2206 1162-1163 NN denotes t
T2207 1163-1164 HYPH denotes -
T2204 1164-1168 NN denotes test
T2208 1168-1170 , denotes ,
T2209 1170-1175 NN denotes means
T2210 1176-1179 CC denotes and
T2211 1180-1188 JJ denotes standard
T2212 1189-1198 NN denotes deviation
T2213 1198-1200 , denotes ,
T2214 1200-1205 VBG denotes using
T2215 1206-1209 DT denotes the
T2217 1210-1221 JJ denotes statistical
T2218 1222-1230 NN denotes software
T2216 1231-1238 NN denotes package
T2219 1239-1247 NN denotes StatMost
T2220 1248-1249 -LRB- denotes (
T2222 1249-1257 NNP denotes Dataxiom
T2224 1258-1266 NNP denotes Software
T2223 1267-1271 NNP denotes Inc.
T2225 1271-1273 , denotes ,
T2226 1273-1276 NNP denotes Los
T2221 1277-1284 NNP denotes Angeles
T2227 1284-1286 , denotes ,
T2228 1286-1288 NNP denotes CA
T2229 1288-1289 -RRB- denotes )
T2230 1289-1290 . denotes .
T2289 1292-1300 JJ denotes Confocal
T2290 1301-1311 NN denotes microscopy
T2291 1311-1436 sentence denotes Lenses were vibratome sectioned into 100–200 μm thick sections along the optical (anterior to posterior) or equatorial axes.
T2292 1312-1318 NNS denotes Lenses
T2294 1319-1323 VBD denotes were
T2295 1324-1333 NN denotes vibratome
T2293 1334-1343 VBN denotes sectioned
T2296 1344-1348 IN denotes into
T2297 1349-1352 CD denotes 100
T2299 1352-1353 SYM denotes –
T2298 1353-1356 CD denotes 200
T2300 1357-1359 NN denotes μm
T2301 1360-1365 JJ denotes thick
T2302 1366-1374 NNS denotes sections
T2303 1375-1380 IN denotes along
T2304 1381-1384 DT denotes the
T2306 1385-1392 JJ denotes optical
T2307 1393-1394 -LRB- denotes (
T2308 1394-1402 JJ denotes anterior
T2309 1403-1405 IN denotes to
T2310 1406-1415 JJ denotes posterior
T2311 1415-1416 -RRB- denotes )
T2312 1417-1419 CC denotes or
T2313 1420-1430 JJ denotes equatorial
T2305 1431-1435 NNS denotes axes
T2314 1435-1436 . denotes .
T2315 1436-1662 sentence denotes Thick sections were fixed in 2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer pH 7.2 at RT for 24 hrs then washed several times in Tris buffered saline (0.5 M Tris, 150 mM NaCl, pH 7.4).
T2316 1437-1442 JJ denotes Thick
T2317 1443-1451 NNS denotes sections
T2319 1452-1456 VBD denotes were
T2318 1457-1462 VBN denotes fixed
T2320 1463-1465 IN denotes in
T2321 1466-1467 CD denotes 2
T2322 1467-1468 NN denotes %
T2324 1469-1470 -LRB- denotes (
T2325 1470-1471 NN denotes w
T2326 1471-1472 SYM denotes /
T2327 1472-1473 NN denotes v
T2328 1473-1474 -RRB- denotes )
T2323 1475-1491 NN denotes paraformaldehyde
T2329 1492-1495 CC denotes and
T2330 1496-1497 CD denotes 2
T2331 1497-1498 NN denotes %
T2332 1499-1513 NN denotes glutaraldehyde
T2333 1514-1515 -LRB- denotes (
T2334 1515-1516 NN denotes v
T2335 1516-1517 SYM denotes /
T2336 1517-1518 NN denotes v
T2337 1518-1519 -RRB- denotes )
T2338 1520-1522 IN denotes in
T2339 1523-1526 CD denotes 0.1
T2340 1527-1528 NN denotes M
T2342 1529-1535 NN denotes sodium
T2343 1536-1546 NN denotes cacodylate
T2341 1547-1553 NN denotes buffer
T2344 1554-1556 NN denotes pH
T2345 1557-1560 CD denotes 7.2
T2346 1561-1563 IN denotes at
T2347 1564-1566 NN denotes RT
T2348 1567-1570 IN denotes for
T2349 1571-1573 CD denotes 24
T2350 1574-1577 NNS denotes hrs
T2351 1578-1582 RB denotes then
T2352 1583-1589 VBD denotes washed
T2353 1590-1597 JJ denotes several
T2354 1598-1603 NNS denotes times
T2355 1604-1606 IN denotes in
T2356 1607-1611 NNP denotes Tris
T2357 1612-1620 VBN denotes buffered
T2358 1621-1627 NN denotes saline
T2359 1628-1629 -LRB- denotes (
T2361 1629-1632 CD denotes 0.5
T2362 1633-1634 NN denotes M
T2363 1635-1639 NNP denotes Tris
T2364 1639-1641 , denotes ,
T2365 1641-1644 CD denotes 150
T2366 1645-1647 NN denotes mM
T2367 1648-1652 NN denotes NaCl
T2368 1652-1654 , denotes ,
T2360 1654-1656 NN denotes pH
T2369 1657-1660 CD denotes 7.4
T2370 1660-1661 -RRB- denotes )
T2371 1661-1662 . denotes .
T2372 1662-1849 sentence denotes To visualize lipid membranes, sections were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI';DiIC18; Molecular Probes) as previously described [24].
T2373 1663-1665 TO denotes To
T2374 1666-1675 VB denotes visualize
T2376 1676-1681 NN denotes lipid
T2377 1682-1691 NNS denotes membranes
T2378 1691-1693 , denotes ,
T2379 1693-1701 NNS denotes sections
T2380 1702-1706 VBD denotes were
T2375 1707-1714 VBN denotes stained
T2381 1715-1719 IN denotes with
T2382 1720-1721 CD denotes 1
T2384 1721-1722 , denotes ,
T2385 1722-1723 CD denotes 1
T2386 1723-1724 SYM denotes '
T2387 1724-1725 HYPH denotes -
T2383 1725-1736 NN denotes dioctadecyl
T2388 1736-1737 HYPH denotes -
T2389 1737-1738 CD denotes 3
T2390 1738-1739 , denotes ,
T2391 1739-1740 CD denotes 3
T2393 1740-1741 , denotes ,
T2394 1741-1742 CD denotes 3
T2395 1742-1743 SYM denotes '
T2396 1743-1744 , denotes ,
T2397 1744-1745 CD denotes 3
T2398 1745-1746 SYM denotes '
T2399 1746-1747 HYPH denotes -
T2400 1747-1774 JJ denotes tetramethylindocarbocyanine
T2392 1775-1786 NN denotes perchlorate
T2401 1787-1788 -LRB- denotes (
T2403 1788-1789 `` denotes '
T2404 1789-1792 NN denotes DiI
T2405 1792-1793 '' denotes '
T2406 1793-1794 : denotes ;
T2407 1794-1800 NN denotes DiIC18
T2408 1800-1801 : denotes ;
T2409 1802-1811 NNP denotes Molecular
T2402 1812-1818 NNP denotes Probes
T2410 1818-1819 -RRB- denotes )
T2411 1820-1822 IN denotes as
T2413 1823-1833 RB denotes previously
T2412 1834-1843 VBN denotes described
T2414 1844-1845 -LRB- denotes [
T2415 1845-1847 CD denotes 24
T2416 1847-1848 -RRB- denotes ]
T2417 1848-1849 . denotes .
T2418 1849-2104 sentence denotes Sections were then washed several times with Tris buffered saline, and nucleic acids were stained by incubating sections in TBS containing 1 μM SYTOX Green (Molecular Probes) for 10 min at RT followed by several washes with TBS prior to confocal imaging.
T2419 1850-1858 NNS denotes Sections
T2421 1859-1863 VBD denotes were
T2422 1864-1868 RB denotes then
T2420 1869-1875 VBN denotes washed
T2423 1876-1883 JJ denotes several
T2424 1884-1889 NNS denotes times
T2425 1890-1894 IN denotes with
T2426 1895-1899 NNP denotes Tris
T2427 1900-1908 VBN denotes buffered
T2428 1909-1915 NN denotes saline
T2429 1915-1917 , denotes ,
T2430 1917-1920 CC denotes and
T2431 1921-1928 JJ denotes nucleic
T2432 1929-1934 NNS denotes acids
T2434 1935-1939 VBD denotes were
T2433 1940-1947 VBN denotes stained
T2435 1948-1950 IN denotes by
T2436 1951-1961 VBG denotes incubating
T2437 1962-1970 NNS denotes sections
T2438 1971-1973 IN denotes in
T2439 1974-1977 NN denotes TBS
T2440 1978-1988 VBG denotes containing
T2441 1989-1990 CD denotes 1
T2442 1991-1993 NN denotes μM
T2444 1994-1999 NN denotes SYTOX
T2443 2000-2005 NN denotes Green
T2445 2006-2007 -LRB- denotes (
T2447 2007-2016 JJ denotes Molecular
T2446 2017-2023 NNP denotes Probes
T2448 2023-2024 -RRB- denotes )
T2449 2025-2028 IN denotes for
T2450 2029-2031 CD denotes 10
T2451 2032-2035 NN denotes min
T2452 2036-2038 IN denotes at
T2453 2039-2041 NN denotes RT
T2454 2042-2050 VBN denotes followed
T2455 2051-2053 IN denotes by
T2456 2054-2061 JJ denotes several
T2457 2062-2068 NNS denotes washes
T2458 2069-2073 IN denotes with
T2459 2074-2077 NN denotes TBS
T2460 2078-2083 RB denotes prior
T2461 2084-2086 IN denotes to
T2462 2087-2095 JJ denotes confocal
T2463 2096-2103 NN denotes imaging
T2464 2103-2104 . denotes .
T2465 2104-2507 sentence denotes Lens sections were viewed on a Zeiss laser scanning confocal microscope model LSM 410 equipped with an Axiovert 100 inverted microscope, an Argon-Krypton 488/568/647 laser, a KP line selection filter, a FT 488/568 Dichroic beam splitter, a FT 560 Dichroic beam splitter, a LP 590 emission filter for viewing DiI, a BP 515–540 emission filter for viewing SYTOX green, and the software package LSM 3.993.
T2466 2105-2109 NNP denotes Lens
T2467 2110-2118 NNS denotes sections
T2469 2119-2123 VBD denotes were
T2468 2124-2130 VBN denotes viewed
T2470 2131-2133 IN denotes on
T2471 2134-2135 DT denotes a
T2473 2136-2141 NNP denotes Zeiss
T2474 2142-2147 NN denotes laser
T2475 2148-2156 NN denotes scanning
T2476 2157-2165 JJ denotes confocal
T2472 2166-2176 NN denotes microscope
T2477 2177-2182 NN denotes model
T2478 2183-2186 NN denotes LSM
T2479 2187-2190 CD denotes 410
T2480 2191-2199 VBN denotes equipped
T2481 2200-2204 IN denotes with
T2482 2205-2207 DT denotes an
T2484 2208-2216 JJ denotes Axiovert
T2485 2217-2220 CD denotes 100
T2486 2221-2229 JJ denotes inverted
T2483 2230-2240 NN denotes microscope
T2487 2240-2242 , denotes ,
T2488 2242-2244 DT denotes an
T2490 2245-2250 JJ denotes Argon
T2492 2250-2251 HYPH denotes -
T2491 2251-2258 JJ denotes Krypton
T2493 2259-2262 CD denotes 488
T2494 2262-2263 HYPH denotes /
T2495 2263-2266 CD denotes 568
T2496 2266-2267 HYPH denotes /
T2497 2267-2270 CD denotes 647
T2489 2271-2276 NN denotes laser
T2498 2276-2278 , denotes ,
T2499 2278-2279 DT denotes a
T2501 2280-2282 NN denotes KP
T2502 2283-2287 NN denotes line
T2503 2288-2297 NN denotes selection
T2500 2298-2304 NN denotes filter
T2504 2304-2306 , denotes ,
T2505 2306-2307 DT denotes a
T2507 2308-2310 NN denotes FT
T2508 2311-2314 CD denotes 488
T2509 2314-2315 HYPH denotes /
T2510 2315-2318 CD denotes 568
T2511 2319-2327 JJ denotes Dichroic
T2512 2328-2332 NN denotes beam
T2506 2333-2341 NN denotes splitter
T2513 2341-2343 , denotes ,
T2514 2343-2344 DT denotes a
T2516 2345-2347 NN denotes FT
T2517 2348-2351 CD denotes 560
T2518 2352-2360 JJ denotes Dichroic
T2519 2361-2365 NN denotes beam
T2515 2366-2374 NN denotes splitter
T2520 2374-2376 , denotes ,
T2521 2376-2377 DT denotes a
T2523 2378-2380 NN denotes LP
T2524 2381-2384 CD denotes 590
T2525 2385-2393 NN denotes emission
T2522 2394-2400 NN denotes filter
T2526 2401-2404 IN denotes for
T2527 2405-2412 VBG denotes viewing
T2528 2413-2416 NNP denotes DiI
T2529 2416-2418 , denotes ,
T2530 2418-2419 DT denotes a
T2532 2420-2422 NN denotes BP
T2533 2423-2426 CD denotes 515
T2534 2426-2427 SYM denotes –
T2535 2427-2430 CD denotes 540
T2536 2431-2439 NN denotes emission
T2531 2440-2446 NN denotes filter
T2537 2447-2450 IN denotes for
T2538 2451-2458 VBG denotes viewing
T2539 2459-2464 NN denotes SYTOX
T2540 2465-2470 NN denotes green
T2541 2470-2472 , denotes ,
T2542 2472-2475 CC denotes and
T2543 2476-2479 DT denotes the
T2545 2480-2488 NN denotes software
T2544 2489-2496 NN denotes package
T2546 2497-2500 NN denotes LSM
T2547 2501-2506 CD denotes 3.993
T2548 2506-2507 . denotes .
T2588 2509-2517 NN denotes Scanning
T2590 2518-2526 NN denotes electron
T2589 2527-2537 NN denotes microscopy
T2591 2537-2713 sentence denotes Lenses bisected along the optical axes were immersion fixed in 2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer pH 7.2 at RT for 24 hrs.
T2592 2538-2544 NNS denotes Lenses
T2594 2545-2553 VBN denotes bisected
T2595 2554-2559 IN denotes along
T2596 2560-2563 DT denotes the
T2598 2564-2571 JJ denotes optical
T2597 2572-2576 NNS denotes axes
T2599 2577-2581 VBD denotes were
T2600 2582-2591 NN denotes immersion
T2593 2592-2597 VBN denotes fixed
T2601 2598-2600 IN denotes in
T2602 2601-2602 CD denotes 2
T2603 2602-2603 NN denotes %
T2605 2604-2605 -LRB- denotes (
T2606 2605-2606 NN denotes w
T2607 2606-2607 SYM denotes /
T2608 2607-2608 NN denotes v
T2609 2608-2609 -RRB- denotes )
T2604 2610-2626 NN denotes paraformaldehyde
T2610 2627-2630 CC denotes and
T2611 2631-2632 CD denotes 2
T2612 2632-2633 NN denotes %
T2613 2634-2648 NN denotes glutaraldehyde
T2614 2649-2650 -LRB- denotes (
T2615 2650-2651 NN denotes v
T2616 2651-2652 SYM denotes /
T2617 2652-2653 NN denotes v
T2618 2653-2654 -RRB- denotes )
T2619 2655-2657 IN denotes in
T2620 2658-2661 CD denotes 0.1
T2621 2662-2663 NN denotes M
T2623 2664-2670 NN denotes sodium
T2622 2671-2681 NN denotes cacodylate
T2624 2682-2688 NN denotes buffer
T2625 2689-2691 NN denotes pH
T2626 2692-2695 CD denotes 7.2
T2627 2696-2698 IN denotes at
T2628 2699-2701 NN denotes RT
T2629 2702-2705 IN denotes for
T2630 2706-2708 CD denotes 24
T2631 2709-2712 NNS denotes hrs
T2632 2712-2713 . denotes .
T2633 2713-2840 sentence denotes Samples were then washed several times with distilled water and postfixed in 1% (v/v) aqueous osmium tetroxide at RT for 1 hr.
T2634 2714-2721 NNS denotes Samples
T2636 2722-2726 VBD denotes were
T2637 2727-2731 RB denotes then
T2635 2732-2738 VBN denotes washed
T2638 2739-2746 JJ denotes several
T2639 2747-2752 NNS denotes times
T2640 2753-2757 IN denotes with
T2641 2758-2767 JJ denotes distilled
T2642 2768-2773 NN denotes water
T2643 2774-2777 CC denotes and
T2644 2778-2787 VBN denotes postfixed
T2645 2788-2790 IN denotes in
T2646 2791-2792 CD denotes 1
T2647 2792-2793 NN denotes %
T2649 2794-2795 -LRB- denotes (
T2650 2795-2796 NN denotes v
T2651 2796-2797 SYM denotes /
T2652 2797-2798 NN denotes v
T2653 2798-2799 -RRB- denotes )
T2654 2800-2807 JJ denotes aqueous
T2655 2808-2814 NN denotes osmium
T2648 2815-2824 NN denotes tetroxide
T2656 2825-2827 IN denotes at
T2657 2828-2830 NN denotes RT
T2658 2831-2834 IN denotes for
T2659 2835-2836 CD denotes 1
T2660 2837-2839 NN denotes hr
T2661 2839-2840 . denotes .
T2662 2840-2914 sentence denotes Lens halves were dehydrated in an ascending ethanol series from 50–100 %.
T2663 2841-2845 NNP denotes Lens
T2664 2846-2852 NNS denotes halves
T2666 2853-2857 VBD denotes were
T2665 2858-2868 VBN denotes dehydrated
T2667 2869-2871 IN denotes in
T2668 2872-2874 DT denotes an
T2670 2875-2884 VBG denotes ascending
T2671 2885-2892 NN denotes ethanol
T2669 2893-2899 NN denotes series
T2672 2900-2904 IN denotes from
T2673 2905-2907 CD denotes 50
T2675 2907-2908 SYM denotes –
T2674 2908-2911 CD denotes 100
T2676 2912-2913 NN denotes %
T2677 2913-2914 . denotes .
T2678 2914-3061 sentence denotes Once lens halves were in 100% ethanol, they were critical point dried in carbon dioxide in a Samdri-790B (Tousimis Research Corp., Rockville, MD).
T2679 2915-2919 IN denotes Once
T2681 2920-2924 NN denotes lens
T2682 2925-2931 NNS denotes halves
T2680 2932-2936 VBD denotes were
T2684 2937-2939 IN denotes in
T2685 2940-2943 CD denotes 100
T2686 2943-2944 NN denotes %
T2687 2945-2952 NN denotes ethanol
T2688 2952-2954 , denotes ,
T2689 2954-2958 PRP denotes they
T2690 2959-2963 VBD denotes were
T2691 2964-2972 JJ denotes critical
T2692 2973-2978 NN denotes point
T2683 2979-2984 VBN denotes dried
T2693 2985-2987 IN denotes in
T2694 2988-2994 NN denotes carbon
T2695 2995-3002 NN denotes dioxide
T2696 3003-3005 IN denotes in
T2697 3006-3007 DT denotes a
T2699 3008-3014 NN denotes Samdri
T2700 3014-3015 HYPH denotes -
T2698 3015-3019 NN denotes 790B
T2701 3020-3021 -LRB- denotes (
T2703 3021-3029 NNP denotes Tousimis
T2704 3030-3038 NNP denotes Research
T2702 3039-3044 NNP denotes Corp.
T2705 3044-3046 , denotes ,
T2706 3046-3055 NNP denotes Rockville
T2707 3055-3057 , denotes ,
T2708 3057-3059 NNP denotes MD
T2709 3059-3060 -RRB- denotes )
T2710 3060-3061 . denotes .
T2711 3061-3149 sentence denotes Critical point dried lens halves were secured on aluminum stubs with double sided tape.
T2712 3062-3070 JJ denotes Critical
T2713 3071-3076 NN denotes point
T2714 3077-3082 VBN denotes dried
T2716 3083-3087 NN denotes lens
T2715 3088-3094 NNS denotes halves
T2718 3095-3099 VBD denotes were
T2717 3100-3107 VBN denotes secured
T2719 3108-3110 IN denotes on
T2720 3111-3119 NN denotes aluminum
T2721 3120-3125 NNS denotes stubs
T2722 3126-3130 IN denotes with
T2723 3131-3137 NN denotes double
T2724 3138-3143 VBN denotes sided
T2725 3144-3148 NN denotes tape
T2726 3148-3149 . denotes .
T2727 3149-3289 sentence denotes Mounted specimens were then sputter coated with gold and viewed on a Hitachi S-3500N scanning electron microscope (Tokyo, Japan) at 1–5 kV.
T2728 3150-3157 VBN denotes Mounted
T2729 3158-3167 NNS denotes specimens
T2731 3168-3172 VBD denotes were
T2732 3173-3177 RB denotes then
T2733 3178-3185 NN denotes sputter
T2730 3186-3192 VBN denotes coated
T2734 3193-3197 IN denotes with
T2735 3198-3202 NN denotes gold
T2736 3203-3206 CC denotes and
T2737 3207-3213 VBN denotes viewed
T2738 3214-3216 IN denotes on
T2739 3217-3218 DT denotes a
T2741 3219-3226 NNP denotes Hitachi
T2743 3227-3228 NN denotes S
T2744 3228-3229 HYPH denotes -
T2742 3229-3234 NN denotes 3500N
T2745 3235-3243 NN denotes scanning
T2746 3244-3252 NN denotes electron
T2740 3253-3263 NN denotes microscope
T2747 3264-3265 -LRB- denotes (
T2748 3265-3270 NNP denotes Tokyo
T2749 3270-3272 , denotes ,
T2750 3272-3277 NNP denotes Japan
T2751 3277-3278 -RRB- denotes )
T2752 3279-3281 IN denotes at
T2753 3282-3283 CD denotes 1
T2755 3283-3284 SYM denotes –
T2754 3284-3285 CD denotes 5
T2756 3286-3288 NN denotes kV
T2757 3288-3289 . denotes .
T2758 3289-3443 sentence denotes This microscope is equipped with Hitachi's patented "Dual-Bias" which allows extremely high emission currents at acceleration voltages of 5 kV and lower.
T2759 3290-3294 DT denotes This
T2760 3295-3305 NN denotes microscope
T2762 3306-3308 VBZ denotes is
T2761 3309-3317 VBN denotes equipped
T2763 3318-3322 IN denotes with
T2764 3323-3330 NNP denotes Hitachi
T2766 3330-3332 POS denotes 's
T2767 3333-3341 VBN denotes patented
T2768 3342-3343 `` denotes "
T2769 3343-3347 NNP denotes Dual
T2770 3347-3348 HYPH denotes -
T2765 3348-3352 NNP denotes Bias
T2771 3352-3353 '' denotes "
T2772 3354-3359 WDT denotes which
T2773 3360-3366 VBZ denotes allows
T2774 3367-3376 RB denotes extremely
T2775 3377-3381 JJ denotes high
T2777 3382-3390 NN denotes emission
T2776 3391-3399 NNS denotes currents
T2778 3400-3402 IN denotes at
T2779 3403-3415 NN denotes acceleration
T2780 3416-3424 NNS denotes voltages
T2781 3425-3427 IN denotes of
T2782 3428-3429 CD denotes 5
T2783 3430-3432 NN denotes kV
T2784 3433-3436 CC denotes and
T2785 3437-3442 JJR denotes lower
T2786 3442-3443 . denotes .
R1281 T1953 T1954 det The,strain
R1282 T1954 T1958 nsubj strain,was
R1283 T1955 T1956 amod wild,type
R1284 T1956 T1954 compound type,strain
R1285 T1957 T1954 compound mouse,strain
R1286 T1959 T1954 acl used,strain
R1287 T1960 T1959 prep in,used
R1288 T1961 T1962 det this,study
R1289 T1962 T1960 pobj study,in
R1290 T1963 T1964 det the,mouse
R1291 T1964 T1958 attr mouse,was
R1292 T1965 T1964 compound 129SvEvTac,mouse
R1293 T1966 T1958 punct .,was
R1294 T1968 T1969 nsubj Lenses,were
R1295 T1970 T1968 acl examined,Lenses
R1296 T1971 T1969 prep from,were
R1297 T1972 T1973 nummod 5,wk
R1298 T1973 T1990 npadvmod wk,old
R1299 T1974 T1975 punct (,lenses
R1300 T1975 T1972 parataxis lenses,5
R1301 T1976 T1975 nummod 8,lenses
R1302 T1977 T1975 punct ),lenses
R1303 T1978 T1972 punct ", ",5
R1304 T1979 T1972 conj 46,5
R1305 T1980 T1981 punct (,lenses
R1306 T1981 T1979 parataxis lenses,46
R1307 T1982 T1981 nummod 8,lenses
R1308 T1983 T1981 punct ),lenses
R1309 T1984 T1979 cc and,46
R1310 T1985 T1979 conj 72,46
R1311 T1986 T1987 punct (,lenses
R1312 T1987 T1985 parataxis lenses,72
R1313 T1988 T1987 nummod 6,lenses
R1314 T1989 T1987 punct ),lenses
R1315 T1990 T1991 amod old,mice
R1316 T1991 T1971 pobj mice,from
R1317 T1992 T1969 punct .,were
R1318 T1994 T1995 compound AlphaA,BKO
R1319 T1995 T1997 nsubjpass BKO,generated
R1320 T1996 T1995 punct /,BKO
R1321 T1998 T1997 auxpass was,generated
R1322 T1999 T1997 prep by,generated
R1323 T2000 T2001 dep cross,breeding
R1324 T2001 T1999 pcomp breeding,by
R1325 T2002 T2003 nmod alphaAKO,127
R1326 T2003 T2005 nmod 127,mice
R1327 T2004 T2003 punct -,127
R1328 T2005 T2001 dobj mice,breeding
R1329 T2006 T2007 punct [,22
R1330 T2007 T2003 parataxis 22,127
R1331 T2008 T2007 punct ],22
R1332 T2009 T2003 cc and,127
R1333 T2010 T2011 compound alphaBKO,168
R1334 T2011 T2003 conj 168,127
R1335 T2012 T2011 punct -,168
R1336 T2013 T2014 punct [,23
R1337 T2014 T2005 parataxis 23,mice
R1338 T2015 T2014 punct ],23
R1339 T2016 T2001 punct ", ",breeding
R1340 T2017 T2018 advmod also,in
R1341 T2018 T2001 prep in,breeding
R1342 T2019 T2020 det a,background
R1343 T2020 T2018 pobj background,in
R1344 T2021 T2020 compound 129Sv,background
R1345 T2022 T1997 punct .,generated
R1346 T2024 T2025 det These,mice
R1347 T2025 T2026 nsubj mice,lack
R1348 T2027 T2026 advmod also,lack
R1349 T2028 T2029 det the,product
R1350 T2029 T2026 dobj product,lack
R1351 T2030 T2029 compound HSPB2,product
R1352 T2031 T2029 compound gene,product
R1353 T2032 T2033 punct [,23
R1354 T2033 T2026 parataxis 23,lack
R1355 T2034 T2033 punct ],23
R1356 T2035 T2026 punct .,lack
R1357 T2037 T2038 nsubj Lenses,were
R1358 T2039 T2037 acl examined,Lenses
R1359 T2040 T2038 prep from,were
R1360 T2041 T2042 nummod 5,wk
R1361 T2042 T2043 npadvmod wk,old
R1362 T2043 T2055 amod old,mice
R1363 T2044 T2045 punct (,lenses
R1364 T2045 T2042 parataxis lenses,wk
R1365 T2046 T2045 nummod 6,lenses
R1366 T2047 T2045 punct ),lenses
R1367 T2048 T2042 cc and,wk
R1368 T2049 T2050 nummod 54,wk
R1369 T2050 T2042 conj wk,wk
R1370 T2051 T2052 punct (,lenses
R1371 T2052 T2050 parataxis lenses,wk
R1372 T2053 T2052 nummod 16,lenses
R1373 T2054 T2052 punct ),lenses
R1374 T2055 T2040 pobj mice,from
R1375 T2056 T2038 punct .,were
R1376 T2058 T2059 det All,animals
R1377 T2059 T2060 nsubjpass animals,treated
R1378 T2061 T2060 auxpass were,treated
R1379 T2062 T2060 prep in,treated
R1380 T2063 T2062 pobj accordance,in
R1381 T2064 T2063 prep with,accordance
R1382 T2065 T2066 det the,Statement
R1383 T2066 T2064 pobj Statement,with
R1384 T2067 T2066 nmod Association,Statement
R1385 T2068 T2067 prep for,Association
R1386 T2069 T2068 pobj Research,for
R1387 T2070 T2069 prep in,Research
R1388 T2071 T2070 pobj Vision,in
R1389 T2072 T2071 cc and,Vision
R1390 T2073 T2071 conj Ophthalmology,Vision
R1391 T2074 T2066 prep for,Statement
R1392 T2075 T2076 det the,Use
R1393 T2076 T2074 pobj Use,for
R1394 T2077 T2076 prep of,Use
R1395 T2078 T2077 pobj Animals,of
R1396 T2079 T2076 prep in,Use
R1397 T2080 T2081 nmod Ophthalmic,Research
R1398 T2081 T2079 pobj Research,in
R1399 T2082 T2080 cc and,Ophthalmic
R1400 T2083 T2080 conj Vision,Ophthalmic
R1401 T2084 T2060 punct .,treated
R1402 T2086 T2087 advmod Immediately,after
R1403 T2087 T2088 prep after,enucleated
R1404 T2089 T2087 pcomp euthanizing,after
R1405 T2090 T2089 dobj animals,euthanizing
R1406 T2091 T2088 punct ", ",enucleated
R1407 T2092 T2088 nsubjpass eyes,enucleated
R1408 T2093 T2088 auxpass were,enucleated
R1409 T2094 T2088 punct .,enucleated
R1410 T2096 T2097 det A,blade
R1411 T2097 T2099 nsubjpass blade,used
R1412 T2098 T2097 compound scalpel,blade
R1413 T2100 T2099 auxpass was,used
R1414 T2101 T2099 advmod then,used
R1415 T2102 T2103 aux to,make
R1416 T2103 T2099 advcl make,used
R1417 T2104 T2105 det a,incision
R1418 T2105 T2103 dobj incision,make
R1419 T2106 T2105 amod small,incision
R1420 T2107 T2105 prep into,incision
R1421 T2108 T2109 det the,chamber
R1422 T2109 T2107 pobj chamber,into
R1423 T2110 T2109 amod anterior,chamber
R1424 T2111 T2109 prep near,chamber
R1425 T2112 T2113 det the,equator
R1426 T2113 T2111 pobj equator,near
R1427 T2114 T2099 cc and,used
R1428 T2115 T2116 advmod then,immersed
R1429 T2116 T2099 conj immersed,used
R1430 T2117 T2118 det both,eyes
R1431 T2118 T2116 nsubjpass eyes,immersed
R1432 T2119 T2118 prep from,eyes
R1433 T2120 T2121 amod individual,animals
R1434 T2121 T2119 pobj animals,from
R1435 T2122 T2116 auxpass were,immersed
R1436 T2123 T2116 prep in,immersed
R1437 T2124 T2125 nummod 5,ml
R1438 T2125 T2126 nmod ml,paraformaldehyde
R1439 T2126 T2123 pobj paraformaldehyde,in
R1440 T2127 T2125 amod fixative,ml
R1441 T2128 T2125 punct (,ml
R1442 T2129 T2130 nummod 2,%
R1443 T2130 T2125 appos %,ml
R1444 T2131 T2132 punct (,w
R1445 T2132 T2130 parataxis w,%
R1446 T2133 T2134 punct /,v
R1447 T2134 T2132 prep v,w
R1448 T2135 T2126 punct ),paraformaldehyde
R1449 T2136 T2126 cc and,paraformaldehyde
R1450 T2137 T2138 nummod 2,%
R1451 T2138 T2139 compound %,glutaraldehyde
R1452 T2139 T2126 conj glutaraldehyde,paraformaldehyde
R1453 T2140 T2141 punct (,v
R1454 T2141 T2139 parataxis v,glutaraldehyde
R1455 T2142 T2143 punct /,v
R1456 T2143 T2141 prep v,v
R1457 T2144 T2141 punct ),v
R1458 T2145 T2126 prep in,paraformaldehyde
R1459 T2146 T2147 nummod 0.1,M
R1460 T2147 T2148 compound M,buffer
R1461 T2148 T2145 pobj buffer,in
R1462 T2149 T2150 compound sodium,cacodylate
R1463 T2150 T2148 compound cacodylate,buffer
R1464 T2151 T2126 punct ", ",paraformaldehyde
R1465 T2152 T2126 npadvmod pH,paraformaldehyde
R1466 T2153 T2152 nummod 7.2,pH
R1467 T2154 T2116 punct ),immersed
R1468 T2155 T2116 punct .,immersed
R1469 T2157 T2158 nsubjpass Eyes,fixed
R1470 T2159 T2158 auxpass were,fixed
R1471 T2160 T2158 prep for,fixed
R1472 T2161 T2162 advmod at,24
R1473 T2162 T2164 nummod 24,hr
R1474 T2163 T2162 advmod least,24
R1475 T2164 T2160 pobj hr,for
R1476 T2165 T2158 prep at,fixed
R1477 T2166 T2167 compound room,temperature
R1478 T2167 T2165 pobj temperature,at
R1479 T2168 T2167 punct (,temperature
R1480 T2169 T2167 appos RT,temperature
R1481 T2170 T2158 punct ),fixed
R1482 T2171 T2172 amod prior,to
R1483 T2172 T2158 prep to,fixed
R1484 T2173 T2172 pobj dissection,to
R1485 T2174 T2173 prep of,dissection
R1486 T2175 T2176 det the,lenses
R1487 T2176 T2174 pobj lenses,of
R1488 T2177 T2158 punct .,fixed
R1489 T2179 T2180 prep At,recorded
R1490 T2181 T2182 det this,time
R1491 T2182 T2179 pobj time,At
R1492 T2183 T2180 punct ", ",recorded
R1493 T2184 T2185 amod equatorial,dimensions
R1494 T2185 T2180 nsubjpass dimensions,recorded
R1495 T2186 T2184 cc and,equatorial
R1496 T2187 T2184 conj axial,equatorial
R1497 T2188 T2185 prep of,dimensions
R1498 T2189 T2188 pobj lenses,of
R1499 T2190 T2185 cc and,dimensions
R1500 T2191 T2192 amod gross,appearance
R1501 T2192 T2185 conj appearance,dimensions
R1502 T2193 T2192 amod lenticular,appearance
R1503 T2194 T2180 auxpass were,recorded
R1504 T2195 T2180 punct .,recorded
R1505 T2197 T2198 amod Statistical,analyses
R1506 T2198 T2199 nsubj analyses,consisted
R1507 T2200 T2198 prep on,analyses
R1508 T2201 T2200 pobj data,on
R1509 T2202 T2199 prep of,consisted
R1510 T2203 T2204 poss Student,test
R1511 T2204 T2202 pobj test,of
R1512 T2205 T2203 case 's,Student
R1513 T2206 T2204 compound t,test
R1514 T2207 T2204 punct -,test
R1515 T2208 T2204 punct ", ",test
R1516 T2209 T2204 conj means,test
R1517 T2210 T2209 cc and,means
R1518 T2211 T2212 amod standard,deviation
R1519 T2212 T2209 conj deviation,means
R1520 T2213 T2199 punct ", ",consisted
R1521 T2214 T2199 advcl using,consisted
R1522 T2215 T2216 det the,package
R1523 T2216 T2214 dobj package,using
R1524 T2217 T2216 amod statistical,package
R1525 T2218 T2216 compound software,package
R1526 T2219 T2216 appos StatMost,package
R1527 T2220 T2221 punct (,Angeles
R1528 T2221 T2199 parataxis Angeles,consisted
R1529 T2222 T2223 compound Dataxiom,Inc.
R1530 T2223 T2221 dep Inc.,Angeles
R1531 T2224 T2223 compound Software,Inc.
R1532 T2225 T2221 punct ", ",Angeles
R1533 T2226 T2221 compound Los,Angeles
R1534 T2227 T2221 punct ", ",Angeles
R1535 T2228 T2221 npadvmod CA,Angeles
R1536 T2229 T2221 punct ),Angeles
R1537 T2230 T2199 punct .,consisted
R1538 T2289 T2290 amod Confocal,microscopy
R1539 T2292 T2293 nsubjpass Lenses,sectioned
R1540 T2294 T2293 auxpass were,sectioned
R1541 T2295 T2293 dep vibratome,sectioned
R1542 T2296 T2293 prep into,sectioned
R1543 T2297 T2298 quantmod 100,200
R1544 T2298 T2300 nummod 200,μm
R1545 T2299 T2298 punct –,200
R1546 T2300 T2301 npadvmod μm,thick
R1547 T2301 T2302 amod thick,sections
R1548 T2302 T2296 pobj sections,into
R1549 T2303 T2293 prep along,sectioned
R1550 T2304 T2305 det the,axes
R1551 T2305 T2303 pobj axes,along
R1552 T2306 T2305 amod optical,axes
R1553 T2307 T2308 punct (,anterior
R1554 T2308 T2306 parataxis anterior,optical
R1555 T2309 T2308 prep to,anterior
R1556 T2310 T2309 amod posterior,to
R1557 T2311 T2308 punct ),anterior
R1558 T2312 T2306 cc or,optical
R1559 T2313 T2306 conj equatorial,optical
R1560 T2314 T2293 punct .,sectioned
R1561 T2316 T2317 amod Thick,sections
R1562 T2317 T2318 nsubjpass sections,fixed
R1563 T2319 T2318 auxpass were,fixed
R1564 T2320 T2318 prep in,fixed
R1565 T2321 T2322 nummod 2,%
R1566 T2322 T2323 nmod %,paraformaldehyde
R1567 T2323 T2320 pobj paraformaldehyde,in
R1568 T2324 T2325 punct (,w
R1569 T2325 T2322 parataxis w,%
R1570 T2326 T2327 punct /,v
R1571 T2327 T2325 prep v,w
R1572 T2328 T2325 punct ),w
R1573 T2329 T2323 cc and,paraformaldehyde
R1574 T2330 T2331 nummod 2,%
R1575 T2331 T2332 compound %,glutaraldehyde
R1576 T2332 T2323 conj glutaraldehyde,paraformaldehyde
R1577 T2333 T2334 punct (,v
R1578 T2334 T2332 parataxis v,glutaraldehyde
R1579 T2335 T2336 punct /,v
R1580 T2336 T2334 prep v,v
R1581 T2337 T2334 punct ),v
R1582 T2338 T2323 prep in,paraformaldehyde
R1583 T2339 T2340 nummod 0.1,M
R1584 T2340 T2341 compound M,buffer
R1585 T2341 T2338 pobj buffer,in
R1586 T2342 T2343 compound sodium,cacodylate
R1587 T2343 T2341 compound cacodylate,buffer
R1588 T2344 T2323 npadvmod pH,paraformaldehyde
R1589 T2345 T2344 nummod 7.2,pH
R1590 T2346 T2318 prep at,fixed
R1591 T2347 T2346 pobj RT,at
R1592 T2348 T2318 prep for,fixed
R1593 T2349 T2350 nummod 24,hrs
R1594 T2350 T2348 pobj hrs,for
R1595 T2351 T2352 advmod then,washed
R1596 T2352 T2318 dep washed,fixed
R1597 T2353 T2354 amod several,times
R1598 T2354 T2352 npadvmod times,washed
R1599 T2355 T2352 prep in,washed
R1600 T2356 T2357 npadvmod Tris,buffered
R1601 T2357 T2358 amod buffered,saline
R1602 T2358 T2355 pobj saline,in
R1603 T2359 T2360 punct (,pH
R1604 T2360 T2358 parataxis pH,saline
R1605 T2361 T2362 nummod 0.5,M
R1606 T2362 T2363 compound M,Tris
R1607 T2363 T2360 dep Tris,pH
R1608 T2364 T2360 punct ", ",pH
R1609 T2365 T2366 nummod 150,mM
R1610 T2366 T2367 compound mM,NaCl
R1611 T2367 T2360 dep NaCl,pH
R1612 T2368 T2360 punct ", ",pH
R1613 T2369 T2360 nummod 7.4,pH
R1614 T2370 T2360 punct ),pH
R1615 T2371 T2318 punct .,fixed
R1616 T2373 T2374 aux To,visualize
R1617 T2374 T2375 advcl visualize,stained
R1618 T2376 T2377 compound lipid,membranes
R1619 T2377 T2374 dobj membranes,visualize
R1620 T2378 T2375 punct ", ",stained
R1621 T2379 T2375 nsubjpass sections,stained
R1622 T2380 T2375 auxpass were,stained
R1623 T2381 T2375 prep with,stained
R1624 T2382 T2383 nummod 1,dioctadecyl
R1625 T2383 T2381 pobj dioctadecyl,with
R1626 T2384 T2382 punct ",",1
R1627 T2385 T2382 appos 1,1
R1628 T2386 T2385 punct ',1
R1629 T2387 T2383 punct -,dioctadecyl
R1630 T2388 T2383 punct -,dioctadecyl
R1631 T2389 T2383 nummod 3,dioctadecyl
R1632 T2390 T2383 punct ",",dioctadecyl
R1633 T2391 T2392 nummod 3,perchlorate
R1634 T2392 T2383 appos perchlorate,dioctadecyl
R1635 T2393 T2391 punct ",",3
R1636 T2394 T2391 appos 3,3
R1637 T2395 T2394 punct ',3
R1638 T2396 T2391 punct ",",3
R1639 T2397 T2391 appos 3,3
R1640 T2398 T2397 punct ',3
R1641 T2399 T2391 punct -,3
R1642 T2400 T2391 amod tetramethylindocarbocyanine,3
R1643 T2401 T2402 punct (,Probes
R1644 T2402 T2383 parataxis Probes,dioctadecyl
R1645 T2403 T2402 punct ',Probes
R1646 T2404 T2402 dep DiI,Probes
R1647 T2405 T2402 punct ',Probes
R1648 T2406 T2402 punct ;,Probes
R1649 T2407 T2402 dep DiIC18,Probes
R1650 T2408 T2402 punct ;,Probes
R1651 T2409 T2402 compound Molecular,Probes
R1652 T2410 T2402 punct ),Probes
R1653 T2411 T2412 mark as,described
R1654 T2412 T2375 advcl described,stained
R1655 T2413 T2412 advmod previously,described
R1656 T2414 T2415 punct [,24
R1657 T2415 T2375 parataxis 24,stained
R1658 T2416 T2415 punct ],24
R1659 T2417 T2375 punct .,stained
R1660 T2419 T2420 nsubjpass Sections,washed
R1661 T2421 T2420 auxpass were,washed
R1662 T2422 T2420 advmod then,washed
R1663 T2423 T2424 amod several,times
R1664 T2424 T2420 npadvmod times,washed
R1665 T2425 T2420 prep with,washed
R1666 T2426 T2427 npadvmod Tris,buffered
R1667 T2427 T2428 amod buffered,saline
R1668 T2428 T2425 pobj saline,with
R1669 T2429 T2420 punct ", ",washed
R1670 T2430 T2420 cc and,washed
R1671 T2431 T2432 amod nucleic,acids
R1672 T2432 T2433 nsubjpass acids,stained
R1673 T2433 T2420 conj stained,washed
R1674 T2434 T2433 auxpass were,stained
R1675 T2435 T2433 prep by,stained
R1676 T2436 T2435 pcomp incubating,by
R1677 T2437 T2436 dobj sections,incubating
R1678 T2438 T2436 prep in,incubating
R1679 T2439 T2438 pobj TBS,in
R1680 T2440 T2439 acl containing,TBS
R1681 T2441 T2442 nummod 1,μM
R1682 T2442 T2443 compound μM,Green
R1683 T2443 T2440 dobj Green,containing
R1684 T2444 T2443 compound SYTOX,Green
R1685 T2445 T2446 punct (,Probes
R1686 T2446 T2440 parataxis Probes,containing
R1687 T2447 T2446 amod Molecular,Probes
R1688 T2448 T2446 punct ),Probes
R1689 T2449 T2436 prep for,incubating
R1690 T2450 T2451 nummod 10,min
R1691 T2451 T2449 pobj min,for
R1692 T2452 T2436 prep at,incubating
R1693 T2453 T2452 pobj RT,at
R1694 T2454 T2436 advcl followed,incubating
R1695 T2455 T2454 agent by,followed
R1696 T2456 T2457 amod several,washes
R1697 T2457 T2455 pobj washes,by
R1698 T2458 T2457 prep with,washes
R1699 T2459 T2458 pobj TBS,with
R1700 T2460 T2457 advmod prior,washes
R1701 T2461 T2460 prep to,prior
R1702 T2462 T2463 amod confocal,imaging
R1703 T2463 T2461 pobj imaging,to
R1704 T2464 T2433 punct .,stained
R1705 T2466 T2467 compound Lens,sections
R1706 T2467 T2468 nsubjpass sections,viewed
R1707 T2469 T2468 auxpass were,viewed
R1708 T2470 T2468 prep on,viewed
R1709 T2471 T2472 det a,microscope
R1710 T2472 T2470 pobj microscope,on
R1711 T2473 T2474 nmod Zeiss,laser
R1712 T2474 T2472 nmod laser,microscope
R1713 T2475 T2472 nmod scanning,microscope
R1714 T2476 T2472 amod confocal,microscope
R1715 T2477 T2478 compound model,LSM
R1716 T2478 T2472 appos LSM,microscope
R1717 T2479 T2478 nummod 410,LSM
R1718 T2480 T2472 acl equipped,microscope
R1719 T2481 T2480 prep with,equipped
R1720 T2482 T2483 det an,microscope
R1721 T2483 T2481 pobj microscope,with
R1722 T2484 T2485 amod Axiovert,100
R1723 T2485 T2483 nummod 100,microscope
R1724 T2486 T2483 amod inverted,microscope
R1725 T2487 T2483 punct ", ",microscope
R1726 T2488 T2489 det an,laser
R1727 T2489 T2483 conj laser,microscope
R1728 T2490 T2491 amod Argon,Krypton
R1729 T2491 T2489 nmod Krypton,laser
R1730 T2492 T2491 punct -,Krypton
R1731 T2493 T2491 nummod 488,Krypton
R1732 T2494 T2491 punct /,Krypton
R1733 T2495 T2491 nummod 568,Krypton
R1734 T2496 T2491 punct /,Krypton
R1735 T2497 T2491 nummod 647,Krypton
R1736 T2498 T2489 punct ", ",laser
R1737 T2499 T2500 det a,filter
R1738 T2500 T2489 conj filter,laser
R1739 T2501 T2500 compound KP,filter
R1740 T2502 T2503 compound line,selection
R1741 T2503 T2500 compound selection,filter
R1742 T2504 T2500 punct ", ",filter
R1743 T2505 T2506 det a,splitter
R1744 T2506 T2500 conj splitter,filter
R1745 T2507 T2506 nmod FT,splitter
R1746 T2508 T2507 nummod 488,FT
R1747 T2509 T2507 punct /,FT
R1748 T2510 T2507 nummod 568,FT
R1749 T2511 T2506 amod Dichroic,splitter
R1750 T2512 T2506 compound beam,splitter
R1751 T2513 T2506 punct ", ",splitter
R1752 T2514 T2515 det a,splitter
R1753 T2515 T2506 conj splitter,splitter
R1754 T2516 T2515 nmod FT,splitter
R1755 T2517 T2516 nummod 560,FT
R1756 T2518 T2515 amod Dichroic,splitter
R1757 T2519 T2515 compound beam,splitter
R1758 T2520 T2515 punct ", ",splitter
R1759 T2521 T2522 det a,filter
R1760 T2522 T2515 conj filter,splitter
R1761 T2523 T2522 nmod LP,filter
R1762 T2524 T2523 nummod 590,LP
R1763 T2525 T2522 compound emission,filter
R1764 T2526 T2522 prep for,filter
R1765 T2527 T2526 pcomp viewing,for
R1766 T2528 T2527 dobj DiI,viewing
R1767 T2529 T2522 punct ", ",filter
R1768 T2530 T2531 det a,filter
R1769 T2531 T2522 conj filter,filter
R1770 T2532 T2531 nmod BP,filter
R1771 T2533 T2532 nummod 515,BP
R1772 T2534 T2535 punct –,540
R1773 T2535 T2532 prep 540,BP
R1774 T2536 T2531 compound emission,filter
R1775 T2537 T2531 prep for,filter
R1776 T2538 T2537 pcomp viewing,for
R1777 T2539 T2540 compound SYTOX,green
R1778 T2540 T2538 dobj green,viewing
R1779 T2541 T2531 punct ", ",filter
R1780 T2542 T2531 cc and,filter
R1781 T2543 T2544 det the,package
R1782 T2544 T2531 conj package,filter
R1783 T2545 T2544 compound software,package
R1784 T2546 T2544 appos LSM,package
R1785 T2547 T2546 nummod 3.993,LSM
R1786 T2548 T2468 punct .,viewed
R1787 T2588 T2589 compound Scanning,microscopy
R1788 T2590 T2589 compound electron,microscopy
R1789 T2592 T2593 nsubjpass Lenses,fixed
R1790 T2594 T2592 acl bisected,Lenses
R1791 T2595 T2594 prep along,bisected
R1792 T2596 T2597 det the,axes
R1793 T2597 T2595 pobj axes,along
R1794 T2598 T2597 amod optical,axes
R1795 T2599 T2593 auxpass were,fixed
R1796 T2600 T2593 dep immersion,fixed
R1797 T2601 T2593 prep in,fixed
R1798 T2602 T2603 nummod 2,%
R1799 T2603 T2604 nmod %,paraformaldehyde
R1800 T2604 T2601 pobj paraformaldehyde,in
R1801 T2605 T2606 punct (,w
R1802 T2606 T2603 parataxis w,%
R1803 T2607 T2608 punct /,v
R1804 T2608 T2606 prep v,w
R1805 T2609 T2606 punct ),w
R1806 T2610 T2604 cc and,paraformaldehyde
R1807 T2611 T2612 nummod 2,%
R1808 T2612 T2613 compound %,glutaraldehyde
R1809 T2613 T2604 conj glutaraldehyde,paraformaldehyde
R1810 T2614 T2615 punct (,v
R1811 T2615 T2613 parataxis v,glutaraldehyde
R1812 T2616 T2617 punct /,v
R1813 T2617 T2615 prep v,v
R1814 T2618 T2615 punct ),v
R1815 T2619 T2604 prep in,paraformaldehyde
R1816 T2620 T2621 nummod 0.1,M
R1817 T2621 T2622 compound M,cacodylate
R1818 T2622 T2624 compound cacodylate,buffer
R1819 T2623 T2622 compound sodium,cacodylate
R1820 T2624 T2619 pobj buffer,in
R1821 T2625 T2624 npadvmod pH,buffer
R1822 T2626 T2625 nummod 7.2,pH
R1823 T2627 T2593 prep at,fixed
R1824 T2628 T2627 pobj RT,at
R1825 T2629 T2593 prep for,fixed
R1826 T2630 T2631 nummod 24,hrs
R1827 T2631 T2629 pobj hrs,for
R1828 T2632 T2593 punct .,fixed
R1829 T2634 T2635 nsubjpass Samples,washed
R1830 T2636 T2635 auxpass were,washed
R1831 T2637 T2635 advmod then,washed
R1832 T2638 T2639 amod several,times
R1833 T2639 T2635 npadvmod times,washed
R1834 T2640 T2635 prep with,washed
R1835 T2641 T2642 amod distilled,water
R1836 T2642 T2640 pobj water,with
R1837 T2643 T2635 cc and,washed
R1838 T2644 T2635 conj postfixed,washed
R1839 T2645 T2644 prep in,postfixed
R1840 T2646 T2647 nummod 1,%
R1841 T2647 T2648 nmod %,tetroxide
R1842 T2648 T2645 pobj tetroxide,in
R1843 T2649 T2650 punct (,v
R1844 T2650 T2648 parataxis v,tetroxide
R1845 T2651 T2652 punct /,v
R1846 T2652 T2650 prep v,v
R1847 T2653 T2650 punct ),v
R1848 T2654 T2648 amod aqueous,tetroxide
R1849 T2655 T2648 compound osmium,tetroxide
R1850 T2656 T2644 prep at,postfixed
R1851 T2657 T2656 pobj RT,at
R1852 T2658 T2644 prep for,postfixed
R1853 T2659 T2660 nummod 1,hr
R1854 T2660 T2658 pobj hr,for
R1855 T2661 T2635 punct .,washed
R1856 T2663 T2664 compound Lens,halves
R1857 T2664 T2665 nsubjpass halves,dehydrated
R1858 T2666 T2665 auxpass were,dehydrated
R1859 T2667 T2665 prep in,dehydrated
R1860 T2668 T2669 det an,series
R1861 T2669 T2667 pobj series,in
R1862 T2670 T2669 amod ascending,series
R1863 T2671 T2669 compound ethanol,series
R1864 T2672 T2669 prep from,series
R1865 T2673 T2674 quantmod 50,100
R1866 T2674 T2676 nummod 100,%
R1867 T2675 T2674 punct –,100
R1868 T2676 T2672 pobj %,from
R1869 T2677 T2665 punct .,dehydrated
R1870 T2679 T2680 mark Once,were
R1871 T2680 T2683 advcl were,dried
R1872 T2681 T2682 compound lens,halves
R1873 T2682 T2680 nsubj halves,were
R1874 T2684 T2680 prep in,were
R1875 T2685 T2686 nummod 100,%
R1876 T2686 T2687 compound %,ethanol
R1877 T2687 T2684 pobj ethanol,in
R1878 T2688 T2683 punct ", ",dried
R1879 T2689 T2683 nsubjpass they,dried
R1880 T2690 T2683 auxpass were,dried
R1881 T2691 T2692 amod critical,point
R1882 T2692 T2683 npadvmod point,dried
R1883 T2693 T2683 prep in,dried
R1884 T2694 T2695 compound carbon,dioxide
R1885 T2695 T2693 pobj dioxide,in
R1886 T2696 T2683 prep in,dried
R1887 T2697 T2698 det a,790B
R1888 T2698 T2696 pobj 790B,in
R1889 T2699 T2698 compound Samdri,790B
R1890 T2700 T2698 punct -,790B
R1891 T2701 T2702 punct (,Corp.
R1892 T2702 T2698 parataxis Corp.,790B
R1893 T2703 T2702 compound Tousimis,Corp.
R1894 T2704 T2702 compound Research,Corp.
R1895 T2705 T2702 punct ", ",Corp.
R1896 T2706 T2702 npadvmod Rockville,Corp.
R1897 T2707 T2702 punct ", ",Corp.
R1898 T2708 T2702 npadvmod MD,Corp.
R1899 T2709 T2702 punct ),Corp.
R1900 T2710 T2683 punct .,dried
R1901 T2712 T2713 amod Critical,point
R1902 T2713 T2714 npadvmod point,dried
R1903 T2714 T2715 amod dried,halves
R1904 T2715 T2717 nsubjpass halves,secured
R1905 T2716 T2715 compound lens,halves
R1906 T2718 T2717 auxpass were,secured
R1907 T2719 T2717 prep on,secured
R1908 T2720 T2721 compound aluminum,stubs
R1909 T2721 T2719 pobj stubs,on
R1910 T2722 T2717 prep with,secured
R1911 T2723 T2724 npadvmod double,sided
R1912 T2724 T2725 amod sided,tape
R1913 T2725 T2722 pobj tape,with
R1914 T2726 T2717 punct .,secured
R1915 T2728 T2729 amod Mounted,specimens
R1916 T2729 T2730 nsubjpass specimens,coated
R1917 T2731 T2730 auxpass were,coated
R1918 T2732 T2730 advmod then,coated
R1919 T2733 T2730 dep sputter,coated
R1920 T2734 T2730 prep with,coated
R1921 T2735 T2734 pobj gold,with
R1922 T2736 T2730 cc and,coated
R1923 T2737 T2730 conj viewed,coated
R1924 T2738 T2737 prep on,viewed
R1925 T2739 T2740 det a,microscope
R1926 T2740 T2738 pobj microscope,on
R1927 T2741 T2742 compound Hitachi,3500N
R1928 T2742 T2740 compound 3500N,microscope
R1929 T2743 T2742 compound S,3500N
R1930 T2744 T2742 punct -,3500N
R1931 T2745 T2740 compound scanning,microscope
R1932 T2746 T2740 compound electron,microscope
R1933 T2747 T2748 punct (,Tokyo
R1934 T2748 T2740 parataxis Tokyo,microscope
R1935 T2749 T2748 punct ", ",Tokyo
R1936 T2750 T2748 npadvmod Japan,Tokyo
R1937 T2751 T2748 punct ),Tokyo
R1938 T2752 T2737 prep at,viewed
R1939 T2753 T2754 quantmod 1,5
R1940 T2754 T2756 nummod 5,kV
R1941 T2755 T2754 punct –,5
R1942 T2756 T2752 pobj kV,at
R1943 T2757 T2730 punct .,coated
R1944 T2759 T2760 det This,microscope
R1945 T2760 T2761 nsubjpass microscope,equipped
R1946 T2762 T2761 auxpass is,equipped
R1947 T2763 T2761 prep with,equipped
R1948 T2764 T2765 poss Hitachi,Bias
R1949 T2765 T2763 pobj Bias,with
R1950 T2766 T2764 case 's,Hitachi
R1951 T2767 T2765 amod patented,Bias
R1952 T2768 T2765 punct """",Bias
R1953 T2769 T2765 compound Dual,Bias
R1954 T2770 T2765 punct -,Bias
R1955 T2771 T2765 punct """",Bias
R1956 T2772 T2773 dep which,allows
R1957 T2773 T2765 relcl allows,Bias
R1958 T2774 T2775 advmod extremely,high
R1959 T2775 T2776 amod high,currents
R1960 T2776 T2773 dobj currents,allows
R1961 T2777 T2776 compound emission,currents
R1962 T2778 T2773 prep at,allows
R1963 T2779 T2780 compound acceleration,voltages
R1964 T2780 T2778 pobj voltages,at
R1965 T2781 T2780 prep of,voltages
R1966 T2782 T2783 nummod 5,kV
R1967 T2783 T2781 pobj kV,of
R1968 T2784 T2783 cc and,kV
R1969 T2785 T2783 conj lower,kV
R1970 T2786 T2761 punct .,equipped