PMC:149350 JSON TXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE

Id	Subject	Object	Predicate	Lexical cue
T137	0-13	JJ	denotes	Morphological
T139	0-94	sentence	denotes	Morphological characterization of the AlphaA- and AlphaB-crystallin double knockout mouse lens
T138	14-30	NN	denotes	characterization
T140	31-33	IN	denotes	of
T141	34-37	DT	denotes	the
T143	38-44	NN	denotes	AlphaA
T145	44-45	HYPH	denotes	-
T146	46-49	CC	denotes	and
T147	50-56	NN	denotes	AlphaB
T148	56-57	HYPH	denotes	-
T144	57-67	NN	denotes	crystallin
T149	68-74	JJ	denotes	double
T150	75-83	NN	denotes	knockout
T151	84-89	NN	denotes	mouse
T142	90-94	NN	denotes	lens
T152	94-95	sentence	denotes
T153	105-115	sentence	denotes	Background
T154	105-115	NN	denotes	Background
T155	115-298	sentence	denotes	One approach to resolving some of the in vivo functions of alpha-crystallin is to generate animal models where one or both of the alpha-crystallin gene products have been eliminated.
T156	116-119	CD	denotes	One
T157	120-128	NN	denotes	approach
T159	129-131	IN	denotes	to
T160	132-141	VBG	denotes	resolving
T161	142-146	DT	denotes	some
T162	147-149	IN	denotes	of
T163	150-153	DT	denotes	the
T165	154-156	FW	denotes	in
T166	157-161	FW	denotes	vivo
T164	162-171	NNS	denotes	functions
T167	172-174	IN	denotes	of
T168	175-180	NN	denotes	alpha
T170	180-181	HYPH	denotes	-
T169	181-191	NN	denotes	crystallin
T158	192-194	VBZ	denotes	is
T171	195-197	TO	denotes	to
T172	198-206	VB	denotes	generate
T173	207-213	NN	denotes	animal
T174	214-220	NNS	denotes	models
T175	221-226	WRB	denotes	where
T177	227-230	CD	denotes	one
T178	231-233	CC	denotes	or
T179	234-238	DT	denotes	both
T180	239-241	IN	denotes	of
T181	242-245	DT	denotes	the
T183	246-251	NN	denotes	alpha
T185	251-252	HYPH	denotes	-
T184	252-262	NN	denotes	crystallin
T186	263-267	NN	denotes	gene
T182	268-276	NNS	denotes	products
T187	277-281	VBP	denotes	have
T188	282-286	VBN	denotes	been
T176	287-297	VBN	denotes	eliminated
T189	297-298	.	denotes	.
T190	298-551	sentence	denotes	In the single alpha-crystallin knockout mice, the remaining alpha-crystallin may fully or partially compensate for some of the functions of the missing protein, especially in the lens, where both alphaA and alphaB are normally expressed at high levels.
T191	299-301	IN	denotes	In
T193	302-305	DT	denotes	the
T195	306-312	JJ	denotes	single
T197	313-318	NN	denotes	alpha
T199	318-319	HYPH	denotes	-
T198	319-329	NN	denotes	crystallin
T196	330-338	NN	denotes	knockout
T194	339-343	NNS	denotes	mice
T200	343-345	,	denotes	,
T201	345-348	DT	denotes	the
T203	349-358	VBG	denotes	remaining
T204	359-364	NN	denotes	alpha
T205	364-365	HYPH	denotes	-
T202	365-375	NN	denotes	crystallin
T206	376-379	MD	denotes	may
T207	380-385	RB	denotes	fully
T208	386-388	CC	denotes	or
T209	389-398	RB	denotes	partially
T192	399-409	VB	denotes	compensate
T210	410-413	IN	denotes	for
T211	414-418	DT	denotes	some
T212	419-421	IN	denotes	of
T213	422-425	DT	denotes	the
T214	426-435	NNS	denotes	functions
T215	436-438	IN	denotes	of
T216	439-442	DT	denotes	the
T218	443-450	VBG	denotes	missing
T217	451-458	NN	denotes	protein
T219	458-460	,	denotes	,
T220	460-470	RB	denotes	especially
T221	471-473	IN	denotes	in
T222	474-477	DT	denotes	the
T223	478-482	NN	denotes	lens
T224	482-484	,	denotes	,
T225	484-489	WRB	denotes	where
T227	490-494	CC	denotes	both
T228	495-501	NN	denotes	alphaA
T229	502-505	CC	denotes	and
T230	506-512	NN	denotes	alphaB
T231	513-516	VBP	denotes	are
T232	517-525	RB	denotes	normally
T226	526-535	VBN	denotes	expressed
T233	536-538	IN	denotes	at
T234	539-543	JJ	denotes	high
T235	544-550	NNS	denotes	levels
T236	550-551	.	denotes	.
T237	551-731	sentence	denotes	The purpose of this study was to characterize gross lenticular morphology in normal mice and mice with the targeted disruption of alphaA- and alphaB-crystallin genes (alphaA/BKO).
T238	552-555	DT	denotes	The
T239	556-563	NN	denotes	purpose
T241	564-566	IN	denotes	of
T242	567-571	DT	denotes	this
T243	572-577	NN	denotes	study
T240	578-581	VBD	denotes	was
T244	582-584	TO	denotes	to
T245	585-597	VB	denotes	characterize
T246	598-603	JJ	denotes	gross
T248	604-614	JJ	denotes	lenticular
T247	615-625	NN	denotes	morphology
T249	626-628	IN	denotes	in
T250	629-635	JJ	denotes	normal
T251	636-640	NNS	denotes	mice
T252	641-644	CC	denotes	and
T253	645-649	NNS	denotes	mice
T254	650-654	IN	denotes	with
T255	655-658	DT	denotes	the
T257	659-667	VBN	denotes	targeted
T256	668-678	NN	denotes	disruption
T258	679-681	IN	denotes	of
T259	682-688	NN	denotes	alphaA
T261	688-689	HYPH	denotes	-
T262	690-693	CC	denotes	and
T263	694-700	NN	denotes	alphaB
T264	700-701	HYPH	denotes	-
T260	701-711	NN	denotes	crystallin
T265	712-717	NNS	denotes	genes
T266	718-719	-LRB-	denotes	(
T267	719-725	NN	denotes	alphaA
T269	725-726	HYPH	denotes	/
T268	726-729	NN	denotes	BKO
T270	729-730	-RRB-	denotes	)
T271	730-731	.	denotes	.
T272	731-740	sentence	denotes	Methods
T273	733-740	NNS	denotes	Methods
T274	740-882	sentence	denotes	Lenses from 129SvEvTac mice and alphaA/BKO mice were examined by standard scanning electron microscopy and confocal microscopy methodologies.
T275	741-747	NNS	denotes	Lenses
T277	748-752	IN	denotes	from
T278	753-763	NN	denotes	129SvEvTac
T279	764-768	NNS	denotes	mice
T280	769-772	CC	denotes	and
T281	773-779	NN	denotes	alphaA
T283	779-780	HYPH	denotes	/
T282	780-783	NN	denotes	BKO
T284	784-788	NNS	denotes	mice
T285	789-793	VBD	denotes	were
T276	794-802	VBN	denotes	examined
T286	803-805	IN	denotes	by
T287	806-814	JJ	denotes	standard
T289	815-823	NN	denotes	scanning
T290	824-832	NN	denotes	electron
T288	833-843	NN	denotes	microscopy
T292	844-847	CC	denotes	and
T293	848-856	JJ	denotes	confocal
T294	857-867	NN	denotes	microscopy
T291	868-881	NNS	denotes	methodologies
T295	881-882	.	denotes	.
T296	882-891	sentence	denotes	Results
T297	884-891	NNS	denotes	Results
T298	891-1026	sentence	denotes	Equatorial and axial (sagittal) dimensions of lenses for alphaA/BKO mice were significantly smaller than age-matched wild type lenses.
T299	892-902	JJ	denotes	Equatorial
T3289	892-902	JJ	denotes	Equatorial
T301	903-906	CC	denotes	and
T3291	903-906	CC	denotes	and
T302	907-912	JJ	denotes	axial
T3292	907-912	JJ	denotes	axial
T304	913-914	-LRB-	denotes	(
T3294	913-914	-LRB-	denotes	(
T303	914-922	JJ	denotes	sagittal
T3293	914-922	JJ	denotes	sagittal
T305	922-923	-RRB-	denotes	)
T3295	922-923	-RRB-	denotes	)
T300	924-934	NNS	denotes	dimensions
T3290	924-934	NNS	denotes	dimensions
T307	935-937	IN	denotes	of
T3297	935-937	IN	denotes	of
T308	938-944	NNS	denotes	lenses
T3298	938-944	NNS	denotes	lenses
T309	945-948	IN	denotes	for
T310	949-955	NN	denotes	alphaA
T312	955-956	HYPH	denotes	/
T311	956-959	NN	denotes	BKO
T313	960-964	NNS	denotes	mice
T306	965-969	VBD	denotes	were
T314	970-983	RB	denotes	significantly
T315	984-991	JJR	denotes	smaller
T316	992-996	IN	denotes	than
T317	997-1000	NN	denotes	age
T319	1000-1001	HYPH	denotes	-
T318	1001-1008	VBN	denotes	matched
T321	1009-1013	JJ	denotes	wild
T322	1014-1018	NN	denotes	type
T320	1019-1025	NNS	denotes	lenses
T323	1025-1026	.	denotes	.
T324	1026-1142	sentence	denotes	No posterior sutures or fiber cells extending to the posterior capsule of the lens were found in alphaA/BKO lenses.
T325	1027-1029	DT	denotes	No
T327	1030-1039	JJ	denotes	posterior
T326	1040-1047	NNS	denotes	sutures
T329	1048-1050	CC	denotes	or
T330	1051-1056	NN	denotes	fiber
T331	1057-1062	NNS	denotes	cells
T332	1063-1072	VBG	denotes	extending
T333	1073-1075	IN	denotes	to
T334	1076-1079	DT	denotes	the
T336	1080-1089	JJ	denotes	posterior
T335	1090-1097	NN	denotes	capsule
T337	1098-1100	IN	denotes	of
T338	1101-1104	DT	denotes	the
T339	1105-1109	NN	denotes	lens
T340	1110-1114	VBD	denotes	were
T328	1115-1120	VBN	denotes	found
T341	1121-1123	IN	denotes	in
T342	1124-1130	NN	denotes	alphaA
T344	1130-1131	HYPH	denotes	/
T343	1131-1134	NN	denotes	BKO
T345	1135-1141	NNS	denotes	lenses
T346	1141-1142	.	denotes	.
T347	1142-1291	sentence	denotes	Ectopical nucleic acid staining was observed in the posterior subcapsular region of 5 wk and anterior subcapsular cortex of 54 wk alphaA/BKO lenses.
T348	1143-1152	JJ	denotes	Ectopical
T350	1153-1160	JJ	denotes	nucleic
T351	1161-1165	NN	denotes	acid
T349	1166-1174	NN	denotes	staining
T353	1175-1178	VBD	denotes	was
T352	1179-1187	VBN	denotes	observed
T354	1188-1190	IN	denotes	in
T355	1191-1194	DT	denotes	the
T357	1195-1204	JJ	denotes	posterior
T358	1205-1216	JJ	denotes	subcapsular
T356	1217-1223	NN	denotes	region
T359	1224-1226	IN	denotes	of
T360	1227-1228	CD	denotes	5
T361	1229-1231	NN	denotes	wk
T362	1232-1235	CC	denotes	and
T363	1236-1244	JJ	denotes	anterior
T365	1245-1256	JJ	denotes	subcapsular
T364	1257-1263	NN	denotes	cortex
T367	1264-1266	IN	denotes	of
T368	1267-1269	CD	denotes	54
T369	1270-1272	NN	denotes	wk
T370	1273-1279	NN	denotes	alphaA
T372	1279-1280	HYPH	denotes	/
T371	1280-1283	NN	denotes	BKO
T366	1284-1290	NNS	denotes	lenses
T373	1290-1291	.	denotes	.
T374	1291-1453	sentence	denotes	Gross morphological differences were also observed in the equatorial/bow, posterior and anterior regions of lenses from alphaA/BKO mice as compared to wild mice.
T375	1292-1297	JJ	denotes	Gross
T377	1298-1311	JJ	denotes	morphological
T376	1312-1323	NNS	denotes	differences
T379	1324-1328	VBD	denotes	were
T380	1329-1333	RB	denotes	also
T378	1334-1342	VBN	denotes	observed
T381	1343-1345	IN	denotes	in
T382	1346-1349	DT	denotes	the
T384	1350-1360	JJ	denotes	equatorial
T386	1360-1361	HYPH	denotes	/
T385	1361-1364	JJ	denotes	bow
T387	1364-1366	,	denotes	,
T388	1366-1375	JJ	denotes	posterior
T389	1376-1379	CC	denotes	and
T390	1380-1388	JJ	denotes	anterior
T383	1389-1396	NNS	denotes	regions
T391	1397-1399	IN	denotes	of
T392	1400-1406	NNS	denotes	lenses
T393	1407-1411	IN	denotes	from
T394	1412-1418	NN	denotes	alphaA
T396	1418-1419	HYPH	denotes	/
T395	1419-1422	NN	denotes	BKO
T397	1423-1427	NNS	denotes	mice
T398	1428-1430	IN	denotes	as
T399	1431-1439	VBN	denotes	compared
T400	1440-1442	IN	denotes	to
T401	1443-1447	JJ	denotes	wild
T402	1448-1452	NNS	denotes	mice
T403	1452-1453	.	denotes	.
T404	1453-1465	sentence	denotes	Conclusion
T405	1455-1465	NN	denotes	Conclusion
T406	1465-1649	sentence	denotes	These results indicated that both alphaA- and alphaB-crystallin are necessary for proper fiber cell formation, and that the absence of alpha-crystallin can lead to cataract formation.
T407	1466-1471	DT	denotes	These
T408	1472-1479	NNS	denotes	results
T409	1480-1489	VBD	denotes	indicated
T410	1490-1494	IN	denotes	that
T412	1495-1499	CC	denotes	both
T413	1500-1506	NN	denotes	alphaA
T415	1506-1507	HYPH	denotes	-
T416	1508-1511	CC	denotes	and
T417	1512-1518	NN	denotes	alphaB
T418	1518-1519	HYPH	denotes	-
T414	1519-1529	NN	denotes	crystallin
T411	1530-1533	VBP	denotes	are
T419	1534-1543	JJ	denotes	necessary
T420	1544-1547	IN	denotes	for
T421	1548-1554	JJ	denotes	proper
T423	1555-1560	NN	denotes	fiber
T424	1561-1565	NN	denotes	cell
T422	1566-1575	NN	denotes	formation
T425	1575-1577	,	denotes	,
T426	1577-1580	CC	denotes	and
T427	1581-1585	IN	denotes	that
T429	1586-1589	DT	denotes	the
T430	1590-1597	NN	denotes	absence
T431	1598-1600	IN	denotes	of
T432	1601-1606	NN	denotes	alpha
T434	1606-1607	HYPH	denotes	-
T433	1607-1617	NN	denotes	crystallin
T435	1618-1621	MD	denotes	can
T428	1622-1626	VB	denotes	lead
T436	1627-1629	IN	denotes	to
T437	1630-1638	NN	denotes	cataract
T438	1639-1648	NN	denotes	formation
T439	1648-1649	.	denotes	.
T815	1662-1667	NN	denotes	Alpha
T817	1667-1668	HYPH	denotes	-
T816	1668-1678	NN	denotes	Crystallin
T819	1679-1681	VBZ	denotes	is
T818	1682-1691	VBN	denotes	comprised
T820	1692-1694	IN	denotes	of
T821	1695-1698	CD	denotes	two
T822	1699-1711	NNS	denotes	polypeptides
T823	1711-1713	,	denotes	,
T824	1713-1719	NN	denotes	alphaA
T826	1719-1720	HYPH	denotes	-
T825	1720-1730	NN	denotes	crystallin
T827	1731-1732	-LRB-	denotes	(
T828	1732-1738	NN	denotes	alphaA
T829	1738-1739	-RRB-	denotes	)
T830	1740-1743	CC	denotes	and
T831	1744-1750	NN	denotes	alphaB
T833	1750-1751	HYPH	denotes	-
T832	1751-1761	NN	denotes	crystallin
T834	1762-1763	-LRB-	denotes	(
T835	1763-1769	NN	denotes	alphaB
T836	1769-1770	-RRB-	denotes	)
T837	1770-1772	,	denotes	,
T838	1772-1777	WDT	denotes	which
T839	1778-1783	VBP	denotes	share
T840	1784-1786	CD	denotes	55
T841	1786-1787	NN	denotes	%
T843	1788-1793	NN	denotes	amino
T842	1794-1798	NN	denotes	acid
T844	1799-1807	NN	denotes	sequence
T845	1808-1816	NN	denotes	homology
T846	1817-1818	-LRB-	denotes	[
T847	1818-1819	CD	denotes	1
T848	1819-1820	-RRB-	denotes	]
T849	1820-1821	.	denotes	.
T850	1821-1993	sentence	denotes	They are the most abundant proteins in lens fiber cells [2,3] and exist as heteroaggregates of approximately 800 kDa that can undergo inter-aggregate subunit exchange [4].
T851	1822-1826	PRP	denotes	They
T852	1827-1830	VBP	denotes	are
T853	1831-1834	DT	denotes	the
T855	1835-1839	RBS	denotes	most
T856	1840-1848	JJ	denotes	abundant
T854	1849-1857	NN	denotes	proteins
T857	1858-1860	IN	denotes	in
T858	1861-1865	NN	denotes	lens
T859	1866-1871	NN	denotes	fiber
T860	1872-1877	NNS	denotes	cells
T861	1878-1879	-LRB-	denotes	[
T863	1879-1880	CD	denotes	2
T864	1880-1881	,	denotes	,
T862	1881-1882	CD	denotes	3
T865	1882-1883	-RRB-	denotes	]
T866	1884-1887	CC	denotes	and
T867	1888-1893	VBP	denotes	exist
T868	1894-1896	IN	denotes	as
T869	1897-1913	NNS	denotes	heteroaggregates
T870	1914-1916	IN	denotes	of
T871	1917-1930	RB	denotes	approximately
T872	1931-1934	CD	denotes	800
T873	1935-1938	NN	denotes	kDa
T874	1939-1943	WDT	denotes	that
T876	1944-1947	MD	denotes	can
T875	1948-1955	VB	denotes	undergo
T877	1956-1971	JJ	denotes	inter-aggregate
T879	1972-1979	NN	denotes	subunit
T878	1980-1988	NN	denotes	exchange
T880	1989-1990	-LRB-	denotes	[
T881	1990-1991	CD	denotes	4
T882	1991-1992	-RRB-	denotes	]
T883	1992-1993	.	denotes	.
T884	1993-2135	sentence	denotes	The expression of these two proteins in the lens epithelium, however, is not uniform throughout different regions of the anterior epithelium.
T885	1994-1997	DT	denotes	The
T886	1998-2008	NN	denotes	expression
T888	2009-2011	IN	denotes	of
T889	2012-2017	DT	denotes	these
T891	2018-2021	CD	denotes	two
T890	2022-2030	NN	denotes	proteins
T892	2031-2033	IN	denotes	in
T893	2034-2037	DT	denotes	the
T895	2038-2042	NN	denotes	lens
T894	2043-2053	NN	denotes	epithelium
T896	2053-2055	,	denotes	,
T897	2055-2062	RB	denotes	however
T898	2062-2064	,	denotes	,
T887	2064-2066	VBZ	denotes	is
T899	2067-2070	RB	denotes	not
T900	2071-2078	JJ	denotes	uniform
T901	2079-2089	IN	denotes	throughout
T902	2090-2099	JJ	denotes	different
T903	2100-2107	NNS	denotes	regions
T904	2108-2110	IN	denotes	of
T905	2111-2114	DT	denotes	the
T907	2115-2123	JJ	denotes	anterior
T906	2124-2134	NN	denotes	epithelium
T908	2134-2135	.	denotes	.
T909	2135-2294	sentence	denotes	AlphaB expression is detected in central epithelium and increases from the central to elongation zones, where epithelial cells differentiate into fiber cells.
T910	2136-2142	NN	denotes	AlphaB
T911	2143-2153	NN	denotes	expression
T913	2154-2156	VBZ	denotes	is
T912	2157-2165	VBN	denotes	detected
T914	2166-2168	IN	denotes	in
T915	2169-2176	JJ	denotes	central
T916	2177-2187	NN	denotes	epithelium
T917	2188-2191	CC	denotes	and
T918	2192-2201	VBZ	denotes	increases
T919	2202-2206	IN	denotes	from
T920	2207-2210	DT	denotes	the
T921	2211-2218	JJ	denotes	central
T922	2219-2221	IN	denotes	to
T923	2222-2232	NN	denotes	elongation
T924	2233-2238	NNS	denotes	zones
T925	2238-2240	,	denotes	,
T926	2240-2245	WRB	denotes	where
T928	2246-2256	JJ	denotes	epithelial
T929	2257-2262	NNS	denotes	cells
T927	2263-2276	VBP	denotes	differentiate
T930	2277-2281	IN	denotes	into
T931	2282-2287	NN	denotes	fiber
T932	2288-2293	NNS	denotes	cells
T933	2293-2294	.	denotes	.
T934	2294-2354	sentence	denotes	AlphaA, however, is not detected in the central epithelium.
T935	2295-2301	NN	denotes	AlphaA
T937	2301-2303	,	denotes	,
T938	2303-2310	RB	denotes	however
T939	2310-2312	,	denotes	,
T940	2312-2314	VBZ	denotes	is
T941	2315-2318	RB	denotes	not
T936	2319-2327	VBN	denotes	detected
T942	2328-2330	IN	denotes	in
T943	2331-2334	DT	denotes	the
T945	2335-2342	JJ	denotes	central
T944	2343-2353	NN	denotes	epithelium
T946	2353-2354	.	denotes	.
T947	2354-2542	sentence	denotes	The relative proportion of alphaA and alphaB changes from a molar ratio of 1:3 in the pericentral and germative zones to a molar ratio of 3:1 in the elongation zone and fiber cells [2,3].
T948	2355-2358	DT	denotes	The
T950	2359-2367	JJ	denotes	relative
T949	2368-2378	NN	denotes	proportion
T952	2379-2381	IN	denotes	of
T953	2382-2388	NN	denotes	alphaA
T954	2389-2392	CC	denotes	and
T955	2393-2399	NN	denotes	alphaB
T951	2400-2407	VBZ	denotes	changes
T956	2408-2412	IN	denotes	from
T957	2413-2414	DT	denotes	a
T959	2415-2420	JJ	denotes	molar
T958	2421-2426	NN	denotes	ratio
T960	2427-2429	IN	denotes	of
T961	2430-2431	CD	denotes	1
T962	2431-2432	SYM	denotes	:
T963	2432-2433	CD	denotes	3
T964	2434-2436	IN	denotes	in
T965	2437-2440	DT	denotes	the
T967	2441-2452	JJ	denotes	pericentral
T968	2453-2456	CC	denotes	and
T969	2457-2466	JJ	denotes	germative
T966	2467-2472	NNS	denotes	zones
T970	2473-2475	IN	denotes	to
T971	2476-2477	DT	denotes	a
T973	2478-2483	JJ	denotes	molar
T972	2484-2489	NN	denotes	ratio
T974	2490-2492	IN	denotes	of
T975	2493-2494	CD	denotes	3
T976	2494-2495	SYM	denotes	:
T977	2495-2496	CD	denotes	1
T978	2497-2499	IN	denotes	in
T979	2500-2503	DT	denotes	the
T981	2504-2514	NN	denotes	elongation
T980	2515-2519	NN	denotes	zone
T982	2520-2523	CC	denotes	and
T983	2524-2529	NN	denotes	fiber
T984	2530-2535	NNS	denotes	cells
T985	2536-2537	-LRB-	denotes	[
T987	2537-2538	CD	denotes	2
T988	2538-2539	,	denotes	,
T986	2539-2540	CD	denotes	3
T989	2540-2541	-RRB-	denotes	]
T990	2541-2542	.	denotes	.
T991	2542-2698	sentence	denotes	These differences in the relative proportions of alphaA and alphaB within the lens suggest different functions for the two subunits in the developing lens.
T992	2543-2548	DT	denotes	These
T993	2549-2560	NNS	denotes	differences
T995	2561-2563	IN	denotes	in
T996	2564-2567	DT	denotes	the
T998	2568-2576	JJ	denotes	relative
T997	2577-2588	NNS	denotes	proportions
T999	2589-2591	IN	denotes	of
T1000	2592-2598	NN	denotes	alphaA
T1001	2599-2602	CC	denotes	and
T1002	2603-2609	NN	denotes	alphaB
T1003	2610-2616	IN	denotes	within
T1004	2617-2620	DT	denotes	the
T1005	2621-2625	NN	denotes	lens
T994	2626-2633	VBP	denotes	suggest
T1006	2634-2643	JJ	denotes	different
T1007	2644-2653	NNS	denotes	functions
T1008	2654-2657	IN	denotes	for
T1009	2658-2661	DT	denotes	the
T1011	2662-2665	CD	denotes	two
T1010	2666-2674	NNS	denotes	subunits
T1012	2675-2677	IN	denotes	in
T1013	2678-2681	DT	denotes	the
T1015	2682-2692	VBG	denotes	developing
T1014	2693-2697	NN	denotes	lens
T1016	2697-2698	.	denotes	.
T1017	2698-2888	sentence	denotes	Prior to the 1990's, alpha-crystallin was thought to be a structural protein whose major cellular function was to produce a dense solution necessary for the refraction of light in the lens.
T1018	2699-2704	RB	denotes	Prior
T1020	2705-2707	IN	denotes	to
T1021	2708-2711	DT	denotes	the
T1022	2712-2716	CD	denotes	1990
T1023	2716-2718	POS	denotes	's
T1024	2718-2720	,	denotes	,
T1025	2720-2725	NN	denotes	alpha
T1027	2725-2726	HYPH	denotes	-
T1026	2726-2736	NN	denotes	crystallin
T1028	2737-2740	VBD	denotes	was
T1019	2741-2748	VBN	denotes	thought
T1029	2749-2751	TO	denotes	to
T1030	2752-2754	VB	denotes	be
T1031	2755-2756	DT	denotes	a
T1033	2757-2767	JJ	denotes	structural
T1032	2768-2775	NN	denotes	protein
T1034	2776-2781	WP$	denotes	whose
T1036	2782-2787	JJ	denotes	major
T1037	2788-2796	JJ	denotes	cellular
T1035	2797-2805	NN	denotes	function
T1038	2806-2809	VBD	denotes	was
T1039	2810-2812	TO	denotes	to
T1040	2813-2820	VB	denotes	produce
T1041	2821-2822	DT	denotes	a
T1043	2823-2828	JJ	denotes	dense
T1042	2829-2837	NN	denotes	solution
T1044	2838-2847	JJ	denotes	necessary
T1045	2848-2851	IN	denotes	for
T1046	2852-2855	DT	denotes	the
T1047	2856-2866	NN	denotes	refraction
T1048	2867-2869	IN	denotes	of
T1049	2870-2875	NN	denotes	light
T1050	2876-2878	IN	denotes	in
T1051	2879-2882	DT	denotes	the
T1052	2883-2887	NN	denotes	lens
T1053	2887-2888	.	denotes	.
T1054	2888-3124	sentence	denotes	During the early 1990's, alpha-crystallin was shown to act as a molecular chaperone, binding to partially denatured proteins, both in vitro [5] and probably in vivo [6], to inhibit further denaturation and aggregation of lens proteins.
T1055	2889-2895	IN	denotes	During
T1057	2896-2899	DT	denotes	the
T1059	2900-2905	JJ	denotes	early
T1058	2906-2910	CD	denotes	1990
T1060	2910-2912	POS	denotes	's
T1061	2912-2914	,	denotes	,
T1062	2914-2919	NN	denotes	alpha
T1064	2919-2920	HYPH	denotes	-
T1063	2920-2930	NN	denotes	crystallin
T1065	2931-2934	VBD	denotes	was
T1056	2935-2940	VBN	denotes	shown
T1066	2941-2943	TO	denotes	to
T1067	2944-2947	VB	denotes	act
T1068	2948-2950	IN	denotes	as
T1069	2951-2952	DT	denotes	a
T1071	2953-2962	JJ	denotes	molecular
T1070	2963-2972	NN	denotes	chaperone
T1072	2972-2974	,	denotes	,
T1073	2974-2981	VBG	denotes	binding
T1074	2982-2984	IN	denotes	to
T1075	2985-2994	RB	denotes	partially
T1076	2995-3004	VBN	denotes	denatured
T1077	3005-3013	NN	denotes	proteins
T1078	3013-3015	,	denotes	,
T1079	3015-3019	CC	denotes	both
T1081	3020-3022	FW	denotes	in
T1082	3023-3028	FW	denotes	vitro
T1083	3029-3030	-LRB-	denotes	[
T1080	3030-3031	CD	denotes	5
T1084	3031-3032	-RRB-	denotes	]
T1085	3033-3036	CC	denotes	and
T1086	3037-3045	RB	denotes	probably
T1087	3046-3048	FW	denotes	in
T1088	3049-3053	FW	denotes	vivo
T1089	3054-3055	-LRB-	denotes	[
T1090	3055-3056	CD	denotes	6
T1091	3056-3057	-RRB-	denotes	]
T1092	3057-3059	,	denotes	,
T1093	3059-3061	TO	denotes	to
T1094	3062-3069	VB	denotes	inhibit
T1095	3070-3077	JJ	denotes	further
T1096	3078-3090	NN	denotes	denaturation
T1097	3091-3094	CC	denotes	and
T1098	3095-3106	NN	denotes	aggregation
T1099	3107-3109	IN	denotes	of
T1100	3110-3114	NN	denotes	lens
T1101	3115-3123	NN	denotes	proteins
T1102	3123-3124	.	denotes	.
T1103	3124-3279	sentence	denotes	AlphaA and alphaB were also shown to have sequence homology with several other proteins that are members of the small heat shock protein (hsp) family [7].
T1104	3125-3131	NN	denotes	AlphaA
T1106	3132-3135	CC	denotes	and
T1107	3136-3142	NN	denotes	alphaB
T1108	3143-3147	VBD	denotes	were
T1109	3148-3152	RB	denotes	also
T1105	3153-3158	VBN	denotes	shown
T1110	3159-3161	TO	denotes	to
T1111	3162-3166	VB	denotes	have
T1112	3167-3175	NN	denotes	sequence
T1113	3176-3184	NN	denotes	homology
T1114	3185-3189	IN	denotes	with
T1115	3190-3197	JJ	denotes	several
T1117	3198-3203	JJ	denotes	other
T1116	3204-3212	NN	denotes	proteins
T1118	3213-3217	WDT	denotes	that
T1119	3218-3221	VBP	denotes	are
T1120	3222-3229	NNS	denotes	members
T1121	3230-3232	IN	denotes	of
T1122	3233-3236	DT	denotes	the
T1124	3237-3242	JJ	denotes	small
T1125	3243-3247	NN	denotes	heat
T1126	3248-3253	NN	denotes	shock
T1127	3254-3261	NN	denotes	protein
T1128	3262-3263	-LRB-	denotes	(
T1129	3263-3266	NN	denotes	hsp
T1130	3266-3267	-RRB-	denotes	)
T1123	3268-3274	NN	denotes	family
T1131	3275-3276	-LRB-	denotes	[
T1132	3276-3277	CD	denotes	7
T1133	3277-3278	-RRB-	denotes	]
T1134	3278-3279	.	denotes	.
T1135	3279-3386	sentence	denotes	Moreover, expression of alpha-crystallin, particularly alphaB, was shown to not be restricted to the lens.
T1136	3280-3288	RB	denotes	Moreover
T1138	3288-3290	,	denotes	,
T1139	3290-3300	NN	denotes	expression
T1140	3301-3303	IN	denotes	of
T1141	3304-3309	NN	denotes	alpha
T1143	3309-3310	HYPH	denotes	-
T1142	3310-3320	NN	denotes	crystallin
T1144	3320-3322	,	denotes	,
T1145	3322-3334	RB	denotes	particularly
T1146	3335-3341	NN	denotes	alphaB
T1147	3341-3343	,	denotes	,
T1148	3343-3346	VBD	denotes	was
T1137	3347-3352	VBN	denotes	shown
T1149	3353-3355	TO	denotes	to
T1151	3356-3359	RB	denotes	not
T1152	3360-3362	VB	denotes	be
T1150	3363-3373	VBN	denotes	restricted
T1153	3374-3376	IN	denotes	to
T1154	3377-3380	DT	denotes	the
T1155	3381-3385	NN	denotes	lens
T1156	3385-3386	.	denotes	.
T1157	3386-3598	sentence	denotes	AlphaB was found to be expressed at significant levels in a variety of nonlenticular tissues, while alphaA has only been detected in small amounts in a few other tissues such as retina, spleen and thymus [8-12].
T1158	3387-3393	NN	denotes	AlphaB
T1160	3394-3397	VBD	denotes	was
T1159	3398-3403	VBN	denotes	found
T1161	3404-3406	TO	denotes	to
T1163	3407-3409	VB	denotes	be
T1162	3410-3419	VBN	denotes	expressed
T1164	3420-3422	IN	denotes	at
T1165	3423-3434	JJ	denotes	significant
T1166	3435-3441	NNS	denotes	levels
T1167	3442-3444	IN	denotes	in
T1168	3445-3446	DT	denotes	a
T1169	3447-3454	NN	denotes	variety
T1170	3455-3457	IN	denotes	of
T1171	3458-3471	JJ	denotes	nonlenticular
T1172	3472-3479	NNS	denotes	tissues
T1173	3479-3481	,	denotes	,
T1174	3481-3486	IN	denotes	while
T1176	3487-3493	NN	denotes	alphaA
T1177	3494-3497	VBZ	denotes	has
T1178	3498-3502	RB	denotes	only
T1179	3503-3507	VBN	denotes	been
T1175	3508-3516	VBN	denotes	detected
T1180	3517-3519	IN	denotes	in
T1181	3520-3525	JJ	denotes	small
T1182	3526-3533	NNS	denotes	amounts
T1183	3534-3536	IN	denotes	in
T1184	3537-3538	DT	denotes	a
T1186	3539-3542	JJ	denotes	few
T1187	3543-3548	JJ	denotes	other
T1185	3549-3556	NNS	denotes	tissues
T1188	3557-3561	JJ	denotes	such
T1189	3562-3564	IN	denotes	as
T1190	3565-3571	NN	denotes	retina
T1191	3571-3573	,	denotes	,
T1192	3573-3579	NN	denotes	spleen
T1193	3580-3583	CC	denotes	and
T1194	3584-3590	NN	denotes	thymus
T1195	3591-3592	-LRB-	denotes	[
T1196	3592-3593	CD	denotes	8
T1197	3593-3594	SYM	denotes	-
T1198	3594-3596	CD	denotes	12
T1199	3596-3597	-RRB-	denotes	]
T1200	3597-3598	.	denotes	.
T1201	3598-3848	sentence	denotes	Collectively, these findings have challenged the dogma that alpha-crystallin is purely a structural protein necessary for light refraction, and have led to the realization that alpha-crystallin may have a variety of biological functions in the lens.
T1202	3599-3611	RB	denotes	Collectively
T1204	3611-3613	,	denotes	,
T1205	3613-3618	DT	denotes	these
T1206	3619-3627	NNS	denotes	findings
T1207	3628-3632	VBP	denotes	have
T1203	3633-3643	VBN	denotes	challenged
T1208	3644-3647	DT	denotes	the
T1209	3648-3653	NN	denotes	dogma
T1210	3654-3658	IN	denotes	that
T1212	3659-3664	NN	denotes	alpha
T1214	3664-3665	HYPH	denotes	-
T1213	3665-3675	NN	denotes	crystallin
T1211	3676-3678	VBZ	denotes	is
T1215	3679-3685	RB	denotes	purely
T1216	3686-3687	DT	denotes	a
T1218	3688-3698	JJ	denotes	structural
T1217	3699-3706	NN	denotes	protein
T1219	3707-3716	JJ	denotes	necessary
T1220	3717-3720	IN	denotes	for
T1221	3721-3726	JJ	denotes	light
T1222	3727-3737	NN	denotes	refraction
T1223	3737-3739	,	denotes	,
T1224	3739-3742	CC	denotes	and
T1225	3743-3747	VBP	denotes	have
T1226	3748-3751	VBN	denotes	led
T1227	3752-3754	IN	denotes	to
T1228	3755-3758	DT	denotes	the
T1229	3759-3770	NN	denotes	realization
T1230	3771-3775	IN	denotes	that
T1232	3776-3781	NN	denotes	alpha
T1234	3781-3782	HYPH	denotes	-
T1233	3782-3792	NN	denotes	crystallin
T1235	3793-3796	MD	denotes	may
T1231	3797-3801	VB	denotes	have
T1236	3802-3803	DT	denotes	a
T1237	3804-3811	NN	denotes	variety
T1238	3812-3814	IN	denotes	of
T1239	3815-3825	JJ	denotes	biological
T1240	3826-3835	NNS	denotes	functions
T1241	3836-3838	IN	denotes	in
T1242	3839-3842	DT	denotes	the
T1243	3843-3847	NN	denotes	lens
T1244	3847-3848	.	denotes	.
T1245	3848-3959	sentence	denotes	A much broader scope of cellular functions of alpha-crystallin in lens is inferred from in vitro observations.
T1246	3849-3850	DT	denotes	A
T1248	3851-3855	RB	denotes	much
T1249	3856-3863	JJR	denotes	broader
T1247	3864-3869	NN	denotes	scope
T1251	3870-3872	IN	denotes	of
T1252	3873-3881	JJ	denotes	cellular
T1253	3882-3891	NNS	denotes	functions
T1254	3892-3894	IN	denotes	of
T1255	3895-3900	NN	denotes	alpha
T1257	3900-3901	HYPH	denotes	-
T1256	3901-3911	NN	denotes	crystallin
T1258	3912-3914	IN	denotes	in
T1259	3915-3919	NN	denotes	lens
T1260	3920-3922	VBZ	denotes	is
T1250	3923-3931	VBN	denotes	inferred
T1261	3932-3936	IN	denotes	from
T1262	3937-3939	FW	denotes	in
T1263	3940-3945	FW	denotes	vitro
T1264	3946-3958	NNS	denotes	observations
T1265	3958-3959	.	denotes	.
T1266	3959-4046	sentence	denotes	Both alphaA and alphaB can bind specifically to actin, in vitro [13] and in vivo [14].
T1267	3960-3964	CC	denotes	Both
T1268	3965-3971	NN	denotes	alphaA
T1270	3972-3975	CC	denotes	and
T1271	3976-3982	NN	denotes	alphaB
T1272	3983-3986	MD	denotes	can
T1269	3987-3991	VB	denotes	bind
T1273	3992-4004	RB	denotes	specifically
T1274	4005-4007	IN	denotes	to
T1275	4008-4013	NN	denotes	actin
T1276	4013-4015	,	denotes	,
T1277	4015-4017	FW	denotes	in
T1279	4018-4023	FW	denotes	vitro
T1280	4024-4025	-LRB-	denotes	[
T1278	4025-4027	CD	denotes	13
T1281	4027-4028	-RRB-	denotes	]
T1282	4029-4032	CC	denotes	and
T1283	4033-4035	FW	denotes	in
T1285	4036-4040	FW	denotes	vivo
T1286	4041-4042	-LRB-	denotes	[
T1284	4042-4044	CD	denotes	14
T1287	4044-4045	-RRB-	denotes	]
T1288	4045-4046	.	denotes	.
T1289	4046-4257	sentence	denotes	Although actin filament formation has been shown to be necessary for differentiation of lens epithelial cells [15], the significance of alpha-crystallin's interaction with actin in differentiation is not known.
T1290	4047-4055	IN	denotes	Although
T1292	4056-4061	NN	denotes	actin
T1293	4062-4070	NN	denotes	filament
T1294	4071-4080	NN	denotes	formation
T1295	4081-4084	VBZ	denotes	has
T1296	4085-4089	VBN	denotes	been
T1291	4090-4095	VBN	denotes	shown
T1298	4096-4098	TO	denotes	to
T1299	4099-4101	VB	denotes	be
T1300	4102-4111	JJ	denotes	necessary
T1301	4112-4115	IN	denotes	for
T1302	4116-4131	NN	denotes	differentiation
T1303	4132-4134	IN	denotes	of
T1304	4135-4139	NN	denotes	lens
T1306	4140-4150	JJ	denotes	epithelial
T1305	4151-4156	NNS	denotes	cells
T1307	4157-4158	-LRB-	denotes	[
T1308	4158-4160	CD	denotes	15
T1309	4160-4161	-RRB-	denotes	]
T1310	4161-4163	,	denotes	,
T1311	4163-4166	DT	denotes	the
T1312	4167-4179	NN	denotes	significance
T1313	4180-4182	IN	denotes	of
T1314	4183-4188	NN	denotes	alpha
T1316	4188-4189	HYPH	denotes	-
T1315	4189-4199	NN	denotes	crystallin
T1318	4199-4201	POS	denotes	's
T1317	4202-4213	NN	denotes	interaction
T1319	4214-4218	IN	denotes	with
T1320	4219-4224	NN	denotes	actin
T1321	4225-4227	IN	denotes	in
T1322	4228-4243	NN	denotes	differentiation
T1323	4244-4246	VBZ	denotes	is
T1324	4247-4250	RB	denotes	not
T1297	4251-4256	VBN	denotes	known
T1325	4256-4257	.	denotes	.
T1326	4257-4498	sentence	denotes	In the lens, alpha-crystallin also associates with type III intermediate filament proteins and the beaded filament proteins CP49 and CP115, and correct beaded filament assembly has been shown depend on the presence of alpha-crystallin [16].
T1327	4258-4260	IN	denotes	In
T1329	4261-4264	DT	denotes	the
T1330	4265-4269	NN	denotes	lens
T1331	4269-4271	,	denotes	,
T1332	4271-4276	NN	denotes	alpha
T1334	4276-4277	HYPH	denotes	-
T1333	4277-4287	NN	denotes	crystallin
T1335	4288-4292	RB	denotes	also
T1328	4293-4303	VBZ	denotes	associates
T1336	4304-4308	IN	denotes	with
T1337	4309-4313	NN	denotes	type
T1339	4314-4317	CD	denotes	III
T1340	4318-4330	JJ	denotes	intermediate
T1341	4331-4339	NN	denotes	filament
T1338	4340-4348	NN	denotes	proteins
T1342	4349-4352	CC	denotes	and
T1343	4353-4356	DT	denotes	the
T1345	4357-4363	VBN	denotes	beaded
T1346	4364-4372	NN	denotes	filament
T1344	4373-4381	NN	denotes	proteins
T1347	4382-4386	NN	denotes	CP49
T1348	4387-4390	CC	denotes	and
T1349	4391-4396	NN	denotes	CP115
T1350	4396-4398	,	denotes	,
T1351	4398-4401	CC	denotes	and
T1352	4402-4409	JJ	denotes	correct
T1354	4410-4416	VBN	denotes	beaded
T1355	4417-4425	NN	denotes	filament
T1353	4426-4434	NN	denotes	assembly
T1357	4435-4438	VBZ	denotes	has
T1358	4439-4443	VBN	denotes	been
T1356	4444-4449	VBN	denotes	shown
T1359	4450-4456	VB	denotes	depend
T1360	4457-4459	IN	denotes	on
T1361	4460-4463	DT	denotes	the
T1362	4464-4472	NN	denotes	presence
T1363	4473-4475	IN	denotes	of
T1364	4476-4481	NN	denotes	alpha
T1366	4481-4482	HYPH	denotes	-
T1365	4482-4492	NN	denotes	crystallin
T1367	4493-4494	-LRB-	denotes	[
T1368	4494-4496	CD	denotes	16
T1369	4496-4497	-RRB-	denotes	]
T1370	4497-4498	.	denotes	.
T1371	4498-4665	sentence	denotes	Beaded filament mRNA levels are greatly increased in differentiating lens epithelium and have been suggested as a pan-specific marker for lens fiber development [17].
T1372	4499-4505	VBN	denotes	Beaded
T1373	4506-4514	NN	denotes	filament
T1375	4515-4519	NN	denotes	mRNA
T1374	4520-4526	NNS	denotes	levels
T1377	4527-4530	VBP	denotes	are
T1378	4531-4538	RB	denotes	greatly
T1376	4539-4548	VBN	denotes	increased
T1379	4549-4551	IN	denotes	in
T1380	4552-4567	VBG	denotes	differentiating
T1382	4568-4572	NN	denotes	lens
T1381	4573-4583	NN	denotes	epithelium
T1383	4584-4587	CC	denotes	and
T1384	4588-4592	VBP	denotes	have
T1386	4593-4597	VBN	denotes	been
T1385	4598-4607	VBN	denotes	suggested
T1387	4608-4610	IN	denotes	as
T1388	4611-4612	DT	denotes	a
T1390	4613-4625	JJ	denotes	pan-specific
T1389	4626-4632	NN	denotes	marker
T1391	4633-4636	IN	denotes	for
T1392	4637-4641	NN	denotes	lens
T1393	4642-4647	NN	denotes	fiber
T1394	4648-4659	NN	denotes	development
T1395	4660-4661	-LRB-	denotes	[
T1396	4661-4663	CD	denotes	17
T1397	4663-4664	-RRB-	denotes	]
T1398	4664-4665	.	denotes	.
T1399	4665-4738	sentence	denotes	Alpha-crystallin has also been shown to interact directly with DNA [18].
T1400	4666-4671	NNP	denotes	Alpha
T1402	4671-4672	HYPH	denotes	-
T1401	4672-4682	NNP	denotes	crystallin
T1404	4683-4686	VBZ	denotes	has
T1405	4687-4691	RB	denotes	also
T1406	4692-4696	VBN	denotes	been
T1403	4697-4702	VBN	denotes	shown
T1407	4703-4705	TO	denotes	to
T1408	4706-4714	VB	denotes	interact
T1409	4715-4723	RB	denotes	directly
T1410	4724-4728	IN	denotes	with
T1411	4729-4732	NN	denotes	DNA
T1412	4733-4734	-LRB-	denotes	[
T1413	4734-4736	CD	denotes	18
T1414	4736-4737	-RRB-	denotes	]
T1415	4737-4738	.	denotes	.
T1416	4738-4893	sentence	denotes	In transfected CHO cells, alphaB has also been shown to ectopically localize to interphase nuclei, suggesting a role for this protein in the nucleus [19].
T1417	4739-4741	IN	denotes	In
T1419	4742-4753	JJ	denotes	transfected
T1421	4754-4757	NN	denotes	CHO
T1420	4758-4763	NNS	denotes	cells
T1422	4763-4765	,	denotes	,
T1423	4765-4771	NN	denotes	alphaB
T1424	4772-4775	VBZ	denotes	has
T1425	4776-4780	RB	denotes	also
T1426	4781-4785	VBN	denotes	been
T1418	4786-4791	VBN	denotes	shown
T1427	4792-4794	TO	denotes	to
T1429	4795-4806	RB	denotes	ectopically
T1428	4807-4815	VB	denotes	localize
T1430	4816-4818	IN	denotes	to
T1431	4819-4829	NN	denotes	interphase
T1432	4830-4836	NNS	denotes	nuclei
T1433	4836-4838	,	denotes	,
T1434	4838-4848	VBG	denotes	suggesting
T1435	4849-4850	DT	denotes	a
T1436	4851-4855	NN	denotes	role
T1437	4856-4859	IN	denotes	for
T1438	4860-4864	DT	denotes	this
T1439	4865-4872	NN	denotes	protein
T1440	4873-4875	IN	denotes	in
T1441	4876-4879	DT	denotes	the
T1442	4880-4887	NN	denotes	nucleus
T1443	4888-4889	-LRB-	denotes	[
T1444	4889-4891	CD	denotes	19
T1445	4891-4892	-RRB-	denotes	]
T1446	4892-4893	.	denotes	.
T1447	4893-5096	sentence	denotes	A nuclear role for alphaB in the lens was supported by the findings that a subset of lens epithelial cells derived from alphaB knockout mice demonstrated hyperproliferation and genomic instability [20].
T1448	4894-4895	DT	denotes	A
T1450	4896-4903	JJ	denotes	nuclear
T1449	4904-4908	NN	denotes	role
T1452	4909-4912	IN	denotes	for
T1453	4913-4919	NN	denotes	alphaB
T1454	4920-4922	IN	denotes	in
T1455	4923-4926	DT	denotes	the
T1456	4927-4931	NN	denotes	lens
T1457	4932-4935	VBD	denotes	was
T1451	4936-4945	VBN	denotes	supported
T1458	4946-4948	IN	denotes	by
T1459	4949-4952	DT	denotes	the
T1460	4953-4961	NNS	denotes	findings
T1461	4962-4966	IN	denotes	that
T1463	4967-4968	DT	denotes	a
T1464	4969-4975	NN	denotes	subset
T1465	4976-4978	IN	denotes	of
T1466	4979-4983	NN	denotes	lens
T1468	4984-4994	JJ	denotes	epithelial
T1467	4995-5000	NNS	denotes	cells
T1469	5001-5008	VBN	denotes	derived
T1470	5009-5013	IN	denotes	from
T1471	5014-5020	NN	denotes	alphaB
T1473	5021-5029	NN	denotes	knockout
T1472	5030-5034	NNS	denotes	mice
T1462	5035-5047	VBD	denotes	demonstrated
T1474	5048-5066	NN	denotes	hyperproliferation
T1475	5067-5070	CC	denotes	and
T1476	5071-5078	JJ	denotes	genomic
T1477	5079-5090	NN	denotes	instability
T1478	5091-5092	-LRB-	denotes	[
T1479	5092-5094	CD	denotes	20
T1480	5094-5095	-RRB-	denotes	]
T1481	5095-5096	.	denotes	.
T1482	5096-5323	sentence	denotes	In addition, the administration of exogenous alpha-crystallin to primary bovine lens epithelial cell cultures resulted in the formation of lentoid bodies, consistent with a role for these proteins in lens differentiation [21].
T1483	5097-5099	IN	denotes	In
T1485	5100-5108	NN	denotes	addition
T1486	5108-5110	,	denotes	,
T1487	5110-5113	DT	denotes	the
T1488	5114-5128	NN	denotes	administration
T1489	5129-5131	IN	denotes	of
T1490	5132-5141	JJ	denotes	exogenous
T1492	5142-5147	NN	denotes	alpha
T1493	5147-5148	HYPH	denotes	-
T1491	5148-5158	NN	denotes	crystallin
T1494	5159-5161	IN	denotes	to
T1495	5162-5169	JJ	denotes	primary
T1497	5170-5176	JJ	denotes	bovine
T1498	5177-5181	NN	denotes	lens
T1500	5182-5192	JJ	denotes	epithelial
T1499	5193-5197	NN	denotes	cell
T1496	5198-5206	NNS	denotes	cultures
T1484	5207-5215	VBD	denotes	resulted
T1501	5216-5218	IN	denotes	in
T1502	5219-5222	DT	denotes	the
T1503	5223-5232	NN	denotes	formation
T1504	5233-5235	IN	denotes	of
T1505	5236-5243	JJ	denotes	lentoid
T1506	5244-5250	NNS	denotes	bodies
T1507	5250-5252	,	denotes	,
T1508	5252-5262	JJ	denotes	consistent
T1509	5263-5267	IN	denotes	with
T1510	5268-5269	DT	denotes	a
T1511	5270-5274	NN	denotes	role
T1512	5275-5278	IN	denotes	for
T1513	5279-5284	DT	denotes	these
T1514	5285-5293	NN	denotes	proteins
T1515	5294-5296	IN	denotes	in
T1516	5297-5301	NN	denotes	lens
T1517	5302-5317	NN	denotes	differentiation
T1518	5318-5319	-LRB-	denotes	[
T1519	5319-5321	CD	denotes	21
T1520	5321-5322	-RRB-	denotes	]
T1521	5322-5323	.	denotes	.
T1522	5323-5412	sentence	denotes	These findings indicate that alpha-crystallin may have a multitude of in vivo functions.
T1523	5324-5329	DT	denotes	These
T1524	5330-5338	NNS	denotes	findings
T1525	5339-5347	VBP	denotes	indicate
T1526	5348-5352	IN	denotes	that
T1528	5353-5358	NN	denotes	alpha
T1530	5358-5359	HYPH	denotes	-
T1529	5359-5369	NN	denotes	crystallin
T1531	5370-5373	MD	denotes	may
T1527	5374-5378	VB	denotes	have
T1532	5379-5380	DT	denotes	a
T1533	5381-5390	NN	denotes	multitude
T1534	5391-5393	IN	denotes	of
T1535	5394-5396	FW	denotes	in
T1536	5397-5401	FW	denotes	vivo
T1537	5402-5411	NNS	denotes	functions
T1538	5411-5412	.	denotes	.
T1539	5412-5595	sentence	denotes	One approach to resolving some of the in vivo functions of alpha-crystallin is to generate animal models where one or both of the alpha-crystallin gene products have been eliminated.
T1540	5413-5416	CD	denotes	One
T1541	5417-5425	NN	denotes	approach
T1543	5426-5428	IN	denotes	to
T1544	5429-5438	VBG	denotes	resolving
T1545	5439-5443	DT	denotes	some
T1546	5444-5446	IN	denotes	of
T1547	5447-5450	DT	denotes	the
T1549	5451-5453	FW	denotes	in
T1550	5454-5458	FW	denotes	vivo
T1548	5459-5468	NNS	denotes	functions
T1551	5469-5471	IN	denotes	of
T1552	5472-5477	NN	denotes	alpha
T1554	5477-5478	HYPH	denotes	-
T1553	5478-5488	NN	denotes	crystallin
T1542	5489-5491	VBZ	denotes	is
T1555	5492-5494	TO	denotes	to
T1556	5495-5503	VB	denotes	generate
T1557	5504-5510	NN	denotes	animal
T1558	5511-5517	NNS	denotes	models
T1559	5518-5523	WRB	denotes	where
T1561	5524-5527	CD	denotes	one
T1562	5528-5530	CC	denotes	or
T1563	5531-5535	DT	denotes	both
T1564	5536-5538	IN	denotes	of
T1565	5539-5542	DT	denotes	the
T1567	5543-5548	NN	denotes	alpha
T1569	5548-5549	HYPH	denotes	-
T1568	5549-5559	NN	denotes	crystallin
T1570	5560-5564	NN	denotes	gene
T1566	5565-5573	NNS	denotes	products
T1571	5574-5578	VBP	denotes	have
T1572	5579-5583	VBN	denotes	been
T1560	5584-5594	VBN	denotes	eliminated
T1573	5594-5595	.	denotes	.
T1574	5595-5837	sentence	denotes	Brady et al. [22] demonstrated, by targeted disruption of the mouse alphaA gene, that this protein was essential for the maintenance of lens transparency, possibly by maintaining the solubility of alphaB, or associated proteins, in the lens.
T1575	5596-5601	NNP	denotes	Brady
T1577	5602-5604	FW	denotes	et
T1578	5605-5608	FW	denotes	al.
T1579	5609-5610	-LRB-	denotes	[
T1580	5610-5612	CD	denotes	22
T1581	5612-5613	-RRB-	denotes	]
T1576	5614-5626	VBD	denotes	demonstrated
T1582	5626-5628	,	denotes	,
T1583	5628-5630	IN	denotes	by
T1584	5631-5639	VBN	denotes	targeted
T1585	5640-5650	NN	denotes	disruption
T1586	5651-5653	IN	denotes	of
T1587	5654-5657	DT	denotes	the
T1589	5658-5663	NN	denotes	mouse
T1590	5664-5670	NN	denotes	alphaA
T1588	5671-5675	NN	denotes	gene
T1591	5675-5677	,	denotes	,
T1592	5677-5681	IN	denotes	that
T1594	5682-5686	DT	denotes	this
T1595	5687-5694	NN	denotes	protein
T1593	5695-5698	VBD	denotes	was
T1596	5699-5708	JJ	denotes	essential
T1597	5709-5712	IN	denotes	for
T1598	5713-5716	DT	denotes	the
T1599	5717-5728	NN	denotes	maintenance
T1600	5729-5731	IN	denotes	of
T1601	5732-5736	NN	denotes	lens
T1602	5737-5749	NN	denotes	transparency
T1603	5749-5751	,	denotes	,
T1604	5751-5759	RB	denotes	possibly
T1605	5760-5762	IN	denotes	by
T1606	5763-5774	VBG	denotes	maintaining
T1607	5775-5778	DT	denotes	the
T1608	5779-5789	NN	denotes	solubility
T1609	5790-5792	IN	denotes	of
T1610	5793-5799	NN	denotes	alphaB
T1611	5799-5801	,	denotes	,
T1612	5801-5803	CC	denotes	or
T1613	5804-5814	VBN	denotes	associated
T1614	5815-5823	NN	denotes	proteins
T1615	5823-5825	,	denotes	,
T1616	5825-5827	IN	denotes	in
T1617	5828-5831	DT	denotes	the
T1618	5832-5836	NN	denotes	lens
T1619	5836-5837	.	denotes	.
T1620	5837-5957	sentence	denotes	These lenses were also reported to be smaller in equatorial and light axial dimensions than age matched wild type lens.
T1621	5838-5843	DT	denotes	These
T1622	5844-5850	NNS	denotes	lenses
T1624	5851-5855	VBD	denotes	were
T1625	5856-5860	RB	denotes	also
T1623	5861-5869	VBN	denotes	reported
T1626	5870-5872	TO	denotes	to
T1627	5873-5875	VB	denotes	be
T1628	5876-5883	JJR	denotes	smaller
T1629	5884-5886	IN	denotes	in
T1630	5887-5897	JJ	denotes	equatorial
T1632	5898-5901	CC	denotes	and
T1633	5902-5907	JJ	denotes	light
T1634	5908-5913	JJ	denotes	axial
T1631	5914-5924	NNS	denotes	dimensions
T1635	5925-5929	IN	denotes	than
T1636	5930-5933	NN	denotes	age
T1637	5934-5941	VBN	denotes	matched
T1639	5942-5946	JJ	denotes	wild
T1640	5947-5951	NN	denotes	type
T1638	5952-5956	NN	denotes	lens
T1641	5956-5957	.	denotes	.
T1642	5957-6108	sentence	denotes	It was not possible using the techniques employed in the study to determine if the smaller lenses were due to reduced volume or number of fiber cells.
T1643	5958-5960	PRP	denotes	It
T1644	5961-5964	VBD	denotes	was
T1645	5965-5968	RB	denotes	not
T1646	5969-5977	JJ	denotes	possible
T1647	5978-5983	VBG	denotes	using
T1648	5984-5987	DT	denotes	the
T1649	5988-5998	NNS	denotes	techniques
T1650	5999-6007	VBN	denotes	employed
T1651	6008-6010	IN	denotes	in
T1652	6011-6014	DT	denotes	the
T1653	6015-6020	NN	denotes	study
T1654	6021-6023	TO	denotes	to
T1655	6024-6033	VB	denotes	determine
T1656	6034-6036	IN	denotes	if
T1658	6037-6040	DT	denotes	the
T1660	6041-6048	JJR	denotes	smaller
T1659	6049-6055	NNS	denotes	lenses
T1657	6056-6060	VBD	denotes	were
T1661	6061-6064	IN	denotes	due
T1662	6065-6067	IN	denotes	to
T1663	6068-6075	VBN	denotes	reduced
T1664	6076-6082	NN	denotes	volume
T1665	6083-6085	CC	denotes	or
T1666	6086-6092	NN	denotes	number
T1667	6093-6095	IN	denotes	of
T1668	6096-6101	NN	denotes	fiber
T1669	6102-6107	NNS	denotes	cells
T1670	6107-6108	.	denotes	.
T1671	6108-6220	sentence	denotes	Targeted disruption of the mouse alphaB gene resulted in lenses similar in size to aged-matched wild type lens.
T1672	6109-6117	VBN	denotes	Targeted
T1673	6118-6128	NN	denotes	disruption
T1675	6129-6131	IN	denotes	of
T1676	6132-6135	DT	denotes	the
T1678	6136-6141	NN	denotes	mouse
T1679	6142-6148	NN	denotes	alphaB
T1677	6149-6153	NN	denotes	gene
T1674	6154-6162	VBD	denotes	resulted
T1680	6163-6165	IN	denotes	in
T1681	6166-6172	NNS	denotes	lenses
T1682	6173-6180	JJ	denotes	similar
T1683	6181-6183	IN	denotes	in
T1684	6184-6188	NN	denotes	size
T1685	6189-6191	IN	denotes	to
T1686	6192-6196	NN	denotes	aged
T1688	6196-6197	HYPH	denotes	-
T1687	6197-6204	VBN	denotes	matched
T1690	6205-6209	JJ	denotes	wild
T1691	6210-6214	NN	denotes	type
T1689	6215-6219	NN	denotes	lens
T1692	6219-6220	.	denotes	.
T1693	6220-6296	sentence	denotes	Moreover, no cataract formation was observed in the alphaB knockout lenses.
T1694	6221-6229	RB	denotes	Moreover
T1696	6229-6231	,	denotes	,
T1697	6231-6233	DT	denotes	no
T1699	6234-6242	NN	denotes	cataract
T1698	6243-6252	NN	denotes	formation
T1700	6253-6256	VBD	denotes	was
T1695	6257-6265	VBN	denotes	observed
T1701	6266-6268	IN	denotes	in
T1702	6269-6272	DT	denotes	the
T1704	6273-6279	NN	denotes	alphaB
T1705	6280-6288	NN	denotes	knockout
T1703	6289-6295	NNS	denotes	lenses
T1706	6295-6296	.	denotes	.
T1707	6296-6457	sentence	denotes	These animals have muscle cell abnormalities, severe postural anomalies, selective muscle degeneration, and shorter life spans compared to normal controls [23].
T1708	6297-6302	DT	denotes	These
T1709	6303-6310	NNS	denotes	animals
T1710	6311-6315	VBP	denotes	have
T1711	6316-6322	NN	denotes	muscle
T1712	6323-6327	NN	denotes	cell
T1713	6328-6341	NNS	denotes	abnormalities
T1714	6341-6343	,	denotes	,
T1715	6343-6349	JJ	denotes	severe
T1717	6350-6358	JJ	denotes	postural
T1716	6359-6368	NNS	denotes	anomalies
T1718	6368-6370	,	denotes	,
T1719	6370-6379	JJ	denotes	selective
T1721	6380-6386	NN	denotes	muscle
T1720	6387-6399	NN	denotes	degeneration
T1722	6399-6401	,	denotes	,
T1723	6401-6404	CC	denotes	and
T1724	6405-6412	JJR	denotes	shorter
T1726	6413-6417	NN	denotes	life
T1725	6418-6423	NNS	denotes	spans
T1727	6424-6432	VBN	denotes	compared
T1728	6433-6435	IN	denotes	to
T1729	6436-6442	JJ	denotes	normal
T1730	6443-6451	NNS	denotes	controls
T1731	6452-6453	-LRB-	denotes	[
T1732	6453-6455	CD	denotes	23
T1733	6455-6456	-RRB-	denotes	]
T1734	6456-6457	.	denotes	.
T1735	6457-6710	sentence	denotes	In the single alpha-crystallin knockout mice, the remaining alpha-crystallin may fully or partially compensate for some of the functions of the missing protein, especially in the lens, where both alphaA and alphaB are normally expressed at high levels.
T1736	6458-6460	IN	denotes	In
T1738	6461-6464	DT	denotes	the
T1740	6465-6471	JJ	denotes	single
T1742	6472-6477	NN	denotes	alpha
T1744	6477-6478	HYPH	denotes	-
T1743	6478-6488	NN	denotes	crystallin
T1741	6489-6497	NN	denotes	knockout
T1739	6498-6502	NNS	denotes	mice
T1745	6502-6504	,	denotes	,
T1746	6504-6507	DT	denotes	the
T1748	6508-6517	VBG	denotes	remaining
T1749	6518-6523	NN	denotes	alpha
T1750	6523-6524	HYPH	denotes	-
T1747	6524-6534	NN	denotes	crystallin
T1751	6535-6538	MD	denotes	may
T1752	6539-6544	RB	denotes	fully
T1753	6545-6547	CC	denotes	or
T1754	6548-6557	RB	denotes	partially
T1737	6558-6568	VB	denotes	compensate
T1755	6569-6572	IN	denotes	for
T1756	6573-6577	DT	denotes	some
T1757	6578-6580	IN	denotes	of
T1758	6581-6584	DT	denotes	the
T1759	6585-6594	NNS	denotes	functions
T1760	6595-6597	IN	denotes	of
T1761	6598-6601	DT	denotes	the
T1763	6602-6609	VBG	denotes	missing
T1762	6610-6617	NN	denotes	protein
T1764	6617-6619	,	denotes	,
T1765	6619-6629	RB	denotes	especially
T1766	6630-6632	IN	denotes	in
T1767	6633-6636	DT	denotes	the
T1768	6637-6641	NN	denotes	lens
T1769	6641-6643	,	denotes	,
T1770	6643-6648	WRB	denotes	where
T1772	6649-6653	CC	denotes	both
T1773	6654-6660	NN	denotes	alphaA
T1774	6661-6664	CC	denotes	and
T1775	6665-6671	NN	denotes	alphaB
T1776	6672-6675	VBP	denotes	are
T1777	6676-6684	RB	denotes	normally
T1771	6685-6694	VBN	denotes	expressed
T1778	6695-6697	IN	denotes	at
T1779	6698-6702	JJ	denotes	high
T1780	6703-6709	NNS	denotes	levels
T1781	6709-6710	.	denotes	.
T1782	6710-7074	sentence	denotes	The objectives of the current report were to characterize gross morphology of young (5 wk) and old (54 wk) mouse lenses with targeted disruption of both the alphaA and alphaB genes, in comparison to age matched wild type lenses, using scanning electron microscopy (SEM) and confocal microscopy, to elucidate the possible functions of alpha-crystallin in the lens.
T1783	6711-6714	DT	denotes	The
T1784	6715-6725	NNS	denotes	objectives
T1786	6726-6728	IN	denotes	of
T1787	6729-6732	DT	denotes	the
T1789	6733-6740	JJ	denotes	current
T1788	6741-6747	NN	denotes	report
T1785	6748-6752	VBD	denotes	were
T1790	6753-6755	TO	denotes	to
T1791	6756-6768	VB	denotes	characterize
T1792	6769-6774	JJ	denotes	gross
T1793	6775-6785	NN	denotes	morphology
T1794	6786-6788	IN	denotes	of
T1795	6789-6794	JJ	denotes	young
T1797	6795-6796	-LRB-	denotes	(
T1799	6796-6797	CD	denotes	5
T1798	6798-6800	NN	denotes	wk
T1800	6800-6801	-RRB-	denotes	)
T1801	6802-6805	CC	denotes	and
T1802	6806-6809	JJ	denotes	old
T1803	6810-6811	-LRB-	denotes	(
T1805	6811-6813	CD	denotes	54
T1804	6814-6816	NN	denotes	wk
T1806	6816-6817	-RRB-	denotes	)
T1807	6818-6823	NN	denotes	mouse
T1796	6824-6830	NNS	denotes	lenses
T1808	6831-6835	IN	denotes	with
T1809	6836-6844	VBN	denotes	targeted
T1810	6845-6855	NN	denotes	disruption
T1811	6856-6858	IN	denotes	of
T1812	6859-6863	CC	denotes	both
T1814	6864-6867	DT	denotes	the
T1815	6868-6874	NN	denotes	alphaA
T1816	6875-6878	CC	denotes	and
T1817	6879-6885	NN	denotes	alphaB
T1813	6886-6891	NNS	denotes	genes
T1818	6891-6893	,	denotes	,
T1819	6893-6895	IN	denotes	in
T1820	6896-6906	NN	denotes	comparison
T1821	6907-6909	IN	denotes	to
T1822	6910-6913	NN	denotes	age
T1823	6914-6921	VBN	denotes	matched
T1825	6922-6926	JJ	denotes	wild
T1826	6927-6931	NN	denotes	type
T1824	6932-6938	NNS	denotes	lenses
T1827	6938-6940	,	denotes	,
T1828	6940-6945	VBG	denotes	using
T1829	6946-6954	VBG	denotes	scanning
T1831	6955-6963	NN	denotes	electron
T1830	6964-6974	NN	denotes	microscopy
T1832	6975-6976	-LRB-	denotes	(
T1833	6976-6979	NN	denotes	SEM
T1834	6979-6980	-RRB-	denotes	)
T1835	6981-6984	CC	denotes	and
T1836	6985-6993	JJ	denotes	confocal
T1837	6994-7004	NN	denotes	microscopy
T1838	7004-7006	,	denotes	,
T1839	7006-7008	TO	denotes	to
T1840	7009-7018	VB	denotes	elucidate
T1841	7019-7022	DT	denotes	the
T1843	7023-7031	JJ	denotes	possible
T1842	7032-7041	NNS	denotes	functions
T1844	7042-7044	IN	denotes	of
T1845	7045-7050	NN	denotes	alpha
T1847	7050-7051	HYPH	denotes	-
T1846	7051-7061	NN	denotes	crystallin
T1848	7062-7064	IN	denotes	in
T1849	7065-7068	DT	denotes	the
T1850	7069-7073	NN	denotes	lens
T1851	7073-7074	.	denotes	.
T1852	7074-7195	sentence	denotes	The results indicate that alpha-crystallin is necessary for proper fiber cell formation and resulting lens transparency.
T1853	7075-7078	DT	denotes	The
T1854	7079-7086	NNS	denotes	results
T1855	7087-7095	VBP	denotes	indicate
T1856	7096-7100	IN	denotes	that
T1858	7101-7106	NN	denotes	alpha
T1860	7106-7107	HYPH	denotes	-
T1859	7107-7117	NN	denotes	crystallin
T1857	7118-7120	VBZ	denotes	is
T1861	7121-7130	JJ	denotes	necessary
T1862	7131-7134	IN	denotes	for
T1863	7135-7141	JJ	denotes	proper
T1865	7142-7147	NN	denotes	fiber
T1866	7148-7152	NN	denotes	cell
T1864	7153-7162	NN	denotes	formation
T1867	7163-7166	CC	denotes	and
T1868	7167-7176	VBG	denotes	resulting
T1870	7177-7181	NN	denotes	lens
T1869	7182-7194	NN	denotes	transparency
T1871	7194-7195	.	denotes	.
T1951	7206-7212	NNS	denotes	Lenses
T1952	7212-7284	sentence	denotes	The wild type mouse strain used in this study was the 129SvEvTac mouse.
T1953	7213-7216	DT	denotes	The
T1955	7217-7221	JJ	denotes	wild
T1956	7222-7226	NN	denotes	type
T1957	7227-7232	NN	denotes	mouse
T1954	7233-7239	NN	denotes	strain
T1959	7240-7244	VBN	denotes	used
T1960	7245-7247	IN	denotes	in
T1961	7248-7252	DT	denotes	this
T1962	7253-7258	NN	denotes	study
T1958	7259-7262	VBD	denotes	was
T1963	7263-7266	DT	denotes	the
T1965	7267-7277	NN	denotes	129SvEvTac
T1964	7278-7283	NN	denotes	mouse
T1966	7283-7284	.	denotes	.
T1967	7284-7366	sentence	denotes	Lenses examined were from 5(8 lenses), 46(8 lenses) and 72(6 lenses) wk old mice.
T1968	7285-7291	NNS	denotes	Lenses
T1970	7292-7300	VBN	denotes	examined
T1969	7301-7305	VBD	denotes	were
T1971	7306-7310	IN	denotes	from
T1972	7311-7312	CD	denotes	5
T1974	7312-7313	-LRB-	denotes	(
T1976	7313-7314	CD	denotes	8
T1975	7315-7321	NNS	denotes	lenses
T1977	7321-7322	-RRB-	denotes	)
T1978	7322-7324	,	denotes	,
T1979	7324-7326	CD	denotes	46
T1980	7326-7327	-LRB-	denotes	(
T1982	7327-7328	CD	denotes	8
T1981	7329-7335	NNS	denotes	lenses
T1983	7335-7336	-RRB-	denotes	)
T1984	7337-7340	CC	denotes	and
T1985	7341-7343	CD	denotes	72
T1986	7343-7344	-LRB-	denotes	(
T1988	7344-7345	CD	denotes	6
T1987	7346-7352	NNS	denotes	lenses
T1989	7352-7353	-RRB-	denotes	)
T1973	7354-7356	NN	denotes	wk
T1990	7357-7360	JJ	denotes	old
T1991	7361-7365	NNS	denotes	mice
T1992	7365-7366	.	denotes	.
T1993	7366-7483	sentence	denotes	AlphaA/BKO was generated by cross breeding alphaAKO-127 [22] and alphaBKO-168 mice [23], also in a 129Sv background.
T1994	7367-7373	NN	denotes	AlphaA
T1996	7373-7374	HYPH	denotes	/
T1995	7374-7377	NN	denotes	BKO
T1998	7378-7381	VBD	denotes	was
T1997	7382-7391	VBN	denotes	generated
T1999	7392-7394	IN	denotes	by
T2000	7395-7400	NN	denotes	cross
T2001	7401-7409	VBG	denotes	breeding
T2002	7410-7418	NN	denotes	alphaAKO
T2004	7418-7419	HYPH	denotes	-
T2003	7419-7422	NN	denotes	127
T2006	7423-7424	-LRB-	denotes	[
T2007	7424-7426	CD	denotes	22
T2008	7426-7427	-RRB-	denotes	]
T2009	7428-7431	CC	denotes	and
T2010	7432-7440	NN	denotes	alphaBKO
T2012	7440-7441	HYPH	denotes	-
T2011	7441-7444	NN	denotes	168
T2005	7445-7449	NNS	denotes	mice
T2013	7450-7451	-LRB-	denotes	[
T2014	7451-7453	CD	denotes	23
T2015	7453-7454	-RRB-	denotes	]
T2016	7454-7456	,	denotes	,
T2017	7456-7460	RB	denotes	also
T2018	7461-7463	IN	denotes	in
T2019	7464-7465	DT	denotes	a
T2021	7466-7471	NN	denotes	129Sv
T2020	7472-7482	NN	denotes	background
T2022	7482-7483	.	denotes	.
T2023	7483-7533	sentence	denotes	These mice also lack the HSPB2 gene product [23].
T2024	7484-7489	DT	denotes	These
T2025	7490-7494	NNS	denotes	mice
T2027	7495-7499	RB	denotes	also
T2026	7500-7504	VBP	denotes	lack
T2028	7505-7508	DT	denotes	the
T2030	7509-7514	NN	denotes	HSPB2
T2031	7515-7519	NN	denotes	gene
T2029	7520-7527	NN	denotes	product
T2032	7528-7529	-LRB-	denotes	[
T2033	7529-7531	CD	denotes	23
T2034	7531-7532	-RRB-	denotes	]
T2035	7532-7533	.	denotes	.
T2036	7533-7607	sentence	denotes	Lenses examined were from 5 wk (6 lenses) and 54 wk (16 lenses) old mice.
T2037	7534-7540	NNS	denotes	Lenses
T2039	7541-7549	VBN	denotes	examined
T2038	7550-7554	VBD	denotes	were
T2040	7555-7559	IN	denotes	from
T2041	7560-7561	CD	denotes	5
T2042	7562-7564	NN	denotes	wk
T2044	7565-7566	-LRB-	denotes	(
T2046	7566-7567	CD	denotes	6
T2045	7568-7574	NNS	denotes	lenses
T2047	7574-7575	-RRB-	denotes	)
T2048	7576-7579	CC	denotes	and
T2049	7580-7582	CD	denotes	54
T2050	7583-7585	NN	denotes	wk
T2051	7586-7587	-LRB-	denotes	(
T2053	7587-7589	CD	denotes	16
T2052	7590-7596	NNS	denotes	lenses
T2054	7596-7597	-RRB-	denotes	)
T2043	7598-7601	JJ	denotes	old
T2055	7602-7606	NNS	denotes	mice
T2056	7606-7607	.	denotes	.
T2057	7607-7776	sentence	denotes	All animals were treated in accordance with the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmic and Vision Research.
T2058	7608-7611	DT	denotes	All
T2059	7612-7619	NNS	denotes	animals
T2061	7620-7624	VBD	denotes	were
T2060	7625-7632	VBN	denotes	treated
T2062	7633-7635	IN	denotes	in
T2063	7636-7646	NN	denotes	accordance
T2064	7647-7651	IN	denotes	with
T2065	7652-7655	DT	denotes	the
T2067	7656-7667	NNP	denotes	Association
T2068	7668-7671	IN	denotes	for
T2069	7672-7680	NNP	denotes	Research
T2070	7681-7683	IN	denotes	in
T2071	7684-7690	NNP	denotes	Vision
T2072	7691-7694	CC	denotes	and
T2073	7695-7708	NNP	denotes	Ophthalmology
T2066	7709-7718	NNP	denotes	Statement
T2074	7719-7722	IN	denotes	for
T2075	7723-7726	DT	denotes	the
T2076	7727-7730	NNP	denotes	Use
T2077	7731-7733	IN	denotes	of
T2078	7734-7741	NNPS	denotes	Animals
T2079	7742-7744	IN	denotes	in
T2080	7745-7755	NNP	denotes	Ophthalmic
T2082	7756-7759	CC	denotes	and
T2083	7760-7766	NNP	denotes	Vision
T2081	7767-7775	NNP	denotes	Research
T2084	7775-7776	.	denotes	.
T2085	7776-7837	sentence	denotes	Immediately after euthanizing animals, eyes were enucleated.
T2086	7777-7788	RB	denotes	Immediately
T2087	7789-7794	IN	denotes	after
T2089	7795-7806	VBG	denotes	euthanizing
T2090	7807-7814	NNS	denotes	animals
T2091	7814-7816	,	denotes	,
T2092	7816-7820	NNS	denotes	eyes
T2093	7821-7825	VBD	denotes	were
T2088	7826-7836	VBN	denotes	enucleated
T2094	7836-7837	.	denotes	.
T2095	7837-8108	sentence	denotes	A scalpel blade was then used to make a small incision into the anterior chamber near the equator and then both eyes from individual animals were immersed in 5 ml fixative (2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer, pH 7.2).
T2096	7838-7839	DT	denotes	A
T2098	7840-7847	NN	denotes	scalpel
T2097	7848-7853	NN	denotes	blade
T2100	7854-7857	VBD	denotes	was
T2101	7858-7862	RB	denotes	then
T2099	7863-7867	VBN	denotes	used
T2102	7868-7870	TO	denotes	to
T2103	7871-7875	VB	denotes	make
T2104	7876-7877	DT	denotes	a
T2106	7878-7883	JJ	denotes	small
T2105	7884-7892	NN	denotes	incision
T2107	7893-7897	IN	denotes	into
T2108	7898-7901	DT	denotes	the
T2110	7902-7910	JJ	denotes	anterior
T2109	7911-7918	NN	denotes	chamber
T2111	7919-7923	IN	denotes	near
T2112	7924-7927	DT	denotes	the
T2113	7928-7935	NN	denotes	equator
T2114	7936-7939	CC	denotes	and
T2115	7940-7944	RB	denotes	then
T2117	7945-7949	DT	denotes	both
T2118	7950-7954	NNS	denotes	eyes
T2119	7955-7959	IN	denotes	from
T2120	7960-7970	JJ	denotes	individual
T2121	7971-7978	NNS	denotes	animals
T2122	7979-7983	VBD	denotes	were
T2116	7984-7992	VBN	denotes	immersed
T2123	7993-7995	IN	denotes	in
T2124	7996-7997	CD	denotes	5
T2125	7998-8000	NN	denotes	ml
T2127	8001-8009	JJ	denotes	fixative
T2128	8010-8011	-LRB-	denotes	(
T2129	8011-8012	CD	denotes	2
T2130	8012-8013	NN	denotes	%
T2131	8014-8015	-LRB-	denotes	(
T2132	8015-8016	NN	denotes	w
T2133	8016-8017	SYM	denotes	/
T2134	8017-8018	NN	denotes	v
T2135	8018-8019	-RRB-	denotes	)
T2126	8020-8036	NN	denotes	paraformaldehyde
T2136	8037-8040	CC	denotes	and
T2137	8041-8042	CD	denotes	2
T2138	8042-8043	NN	denotes	%
T2139	8044-8058	NN	denotes	glutaraldehyde
T2140	8059-8060	-LRB-	denotes	(
T2141	8060-8061	NN	denotes	v
T2142	8061-8062	SYM	denotes	/
T2143	8062-8063	NN	denotes	v
T2144	8063-8064	-RRB-	denotes	)
T2145	8065-8067	IN	denotes	in
T2146	8068-8071	CD	denotes	0.1
T2147	8072-8073	NN	denotes	M
T2149	8074-8080	NN	denotes	sodium
T2150	8081-8091	NN	denotes	cacodylate
T2148	8092-8098	NN	denotes	buffer
T2151	8098-8100	,	denotes	,
T2152	8100-8102	NN	denotes	pH
T2153	8103-8106	CD	denotes	7.2
T2154	8106-8107	-RRB-	denotes	)
T2155	8107-8108	.	denotes	.
T2156	8108-8203	sentence	denotes	Eyes were fixed for at least 24 hr at room temperature (RT) prior to dissection of the lenses.
T2157	8109-8113	NNS	denotes	Eyes
T2159	8114-8118	VBD	denotes	were
T2158	8119-8124	VBN	denotes	fixed
T2160	8125-8128	IN	denotes	for
T2161	8129-8131	RB	denotes	at
T2163	8132-8137	RBS	denotes	least
T2162	8138-8140	CD	denotes	24
T2164	8141-8143	NNS	denotes	hr
T2165	8144-8146	IN	denotes	at
T2166	8147-8151	NN	denotes	room
T2167	8152-8163	NN	denotes	temperature
T2168	8164-8165	-LRB-	denotes	(
T2169	8165-8167	NN	denotes	RT
T2170	8167-8168	-RRB-	denotes	)
T2171	8169-8174	JJ	denotes	prior
T2172	8175-8177	IN	denotes	to
T2173	8178-8188	NN	denotes	dissection
T2174	8189-8191	IN	denotes	of
T2175	8192-8195	DT	denotes	the
T2176	8196-8202	NNS	denotes	lenses
T2177	8202-8203	.	denotes	.
T2178	8203-8306	sentence	denotes	At this time, equatorial and axial dimensions of lenses and gross lenticular appearance were recorded.
T2179	8204-8206	IN	denotes	At
T2181	8207-8211	DT	denotes	this
T2182	8212-8216	NN	denotes	time
T2183	8216-8218	,	denotes	,
T2184	8218-8228	JJ	denotes	equatorial
T2186	8229-8232	CC	denotes	and
T2187	8233-8238	JJ	denotes	axial
T2185	8239-8249	NNS	denotes	dimensions
T2188	8250-8252	IN	denotes	of
T2189	8253-8259	NNS	denotes	lenses
T2190	8260-8263	CC	denotes	and
T2191	8264-8269	JJ	denotes	gross
T2193	8270-8280	JJ	denotes	lenticular
T2192	8281-8291	NN	denotes	appearance
T2194	8292-8296	VBD	denotes	were
T2180	8297-8305	VBN	denotes	recorded
T2195	8305-8306	.	denotes	.
T2196	8306-8487	sentence	denotes	Statistical analyses on data consisted of Student's t-test, means and standard deviation, using the statistical software package StatMost (Dataxiom Software Inc., Los Angeles, CA).
T2197	8307-8318	JJ	denotes	Statistical
T2198	8319-8327	NNS	denotes	analyses
T2200	8328-8330	IN	denotes	on
T2201	8331-8335	NNS	denotes	data
T2199	8336-8345	VBD	denotes	consisted
T2202	8346-8348	IN	denotes	of
T2203	8349-8356	NN	denotes	Student
T2205	8356-8358	POS	denotes	's
T2206	8359-8360	NN	denotes	t
T2207	8360-8361	HYPH	denotes	-
T2204	8361-8365	NN	denotes	test
T2208	8365-8367	,	denotes	,
T2209	8367-8372	NN	denotes	means
T2210	8373-8376	CC	denotes	and
T2211	8377-8385	JJ	denotes	standard
T2212	8386-8395	NN	denotes	deviation
T2213	8395-8397	,	denotes	,
T2214	8397-8402	VBG	denotes	using
T2215	8403-8406	DT	denotes	the
T2217	8407-8418	JJ	denotes	statistical
T2218	8419-8427	NN	denotes	software
T2216	8428-8435	NN	denotes	package
T2219	8436-8444	NN	denotes	StatMost
T2220	8445-8446	-LRB-	denotes	(
T2222	8446-8454	NNP	denotes	Dataxiom
T2224	8455-8463	NNP	denotes	Software
T2223	8464-8468	NNP	denotes	Inc.
T2225	8468-8470	,	denotes	,
T2226	8470-8473	NNP	denotes	Los
T2221	8474-8481	NNP	denotes	Angeles
T2227	8481-8483	,	denotes	,
T2228	8483-8485	NNP	denotes	CA
T2229	8485-8486	-RRB-	denotes	)
T2230	8486-8487	.	denotes	.
T2289	8489-8497	JJ	denotes	Confocal
T2290	8498-8508	NN	denotes	microscopy
T2291	8508-8633	sentence	denotes	Lenses were vibratome sectioned into 100–200 μm thick sections along the optical (anterior to posterior) or equatorial axes.
T2292	8509-8515	NNS	denotes	Lenses
T2294	8516-8520	VBD	denotes	were
T2295	8521-8530	NN	denotes	vibratome
T2293	8531-8540	VBN	denotes	sectioned
T2296	8541-8545	IN	denotes	into
T2297	8546-8549	CD	denotes	100
T2299	8549-8550	SYM	denotes	–
T2298	8550-8553	CD	denotes	200
T2300	8554-8556	NN	denotes	μm
T2301	8557-8562	JJ	denotes	thick
T2302	8563-8571	NNS	denotes	sections
T2303	8572-8577	IN	denotes	along
T2304	8578-8581	DT	denotes	the
T2306	8582-8589	JJ	denotes	optical
T2307	8590-8591	-LRB-	denotes	(
T2308	8591-8599	JJ	denotes	anterior
T2309	8600-8602	IN	denotes	to
T2310	8603-8612	JJ	denotes	posterior
T2311	8612-8613	-RRB-	denotes	)
T2312	8614-8616	CC	denotes	or
T2313	8617-8627	JJ	denotes	equatorial
T2305	8628-8632	NNS	denotes	axes
T2314	8632-8633	.	denotes	.
T2315	8633-8859	sentence	denotes	Thick sections were fixed in 2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer pH 7.2 at RT for 24 hrs then washed several times in Tris buffered saline (0.5 M Tris, 150 mM NaCl, pH 7.4).
T2316	8634-8639	JJ	denotes	Thick
T2317	8640-8648	NNS	denotes	sections
T2319	8649-8653	VBD	denotes	were
T2318	8654-8659	VBN	denotes	fixed
T2320	8660-8662	IN	denotes	in
T2321	8663-8664	CD	denotes	2
T2322	8664-8665	NN	denotes	%
T2324	8666-8667	-LRB-	denotes	(
T2325	8667-8668	NN	denotes	w
T2326	8668-8669	SYM	denotes	/
T2327	8669-8670	NN	denotes	v
T2328	8670-8671	-RRB-	denotes	)
T2323	8672-8688	NN	denotes	paraformaldehyde
T2329	8689-8692	CC	denotes	and
T2330	8693-8694	CD	denotes	2
T2331	8694-8695	NN	denotes	%
T2332	8696-8710	NN	denotes	glutaraldehyde
T2333	8711-8712	-LRB-	denotes	(
T2334	8712-8713	NN	denotes	v
T2335	8713-8714	SYM	denotes	/
T2336	8714-8715	NN	denotes	v
T2337	8715-8716	-RRB-	denotes	)
T2338	8717-8719	IN	denotes	in
T2339	8720-8723	CD	denotes	0.1
T2340	8724-8725	NN	denotes	M
T2342	8726-8732	NN	denotes	sodium
T2343	8733-8743	NN	denotes	cacodylate
T2341	8744-8750	NN	denotes	buffer
T2344	8751-8753	NN	denotes	pH
T2345	8754-8757	CD	denotes	7.2
T2346	8758-8760	IN	denotes	at
T2347	8761-8763	NN	denotes	RT
T2348	8764-8767	IN	denotes	for
T2349	8768-8770	CD	denotes	24
T2350	8771-8774	NNS	denotes	hrs
T2351	8775-8779	RB	denotes	then
T2352	8780-8786	VBD	denotes	washed
T2353	8787-8794	JJ	denotes	several
T2354	8795-8800	NNS	denotes	times
T2355	8801-8803	IN	denotes	in
T2356	8804-8808	NNP	denotes	Tris
T2357	8809-8817	VBN	denotes	buffered
T2358	8818-8824	NN	denotes	saline
T2359	8825-8826	-LRB-	denotes	(
T2361	8826-8829	CD	denotes	0.5
T2362	8830-8831	NN	denotes	M
T2363	8832-8836	NNP	denotes	Tris
T2364	8836-8838	,	denotes	,
T2365	8838-8841	CD	denotes	150
T2366	8842-8844	NN	denotes	mM
T2367	8845-8849	NN	denotes	NaCl
T2368	8849-8851	,	denotes	,
T2360	8851-8853	NN	denotes	pH
T2369	8854-8857	CD	denotes	7.4
T2370	8857-8858	-RRB-	denotes	)
T2371	8858-8859	.	denotes	.
T2372	8859-9046	sentence	denotes	To visualize lipid membranes, sections were stained with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate ('DiI';DiIC18; Molecular Probes) as previously described [24].
T2373	8860-8862	TO	denotes	To
T2374	8863-8872	VB	denotes	visualize
T2376	8873-8878	NN	denotes	lipid
T2377	8879-8888	NNS	denotes	membranes
T2378	8888-8890	,	denotes	,
T2379	8890-8898	NNS	denotes	sections
T2380	8899-8903	VBD	denotes	were
T2375	8904-8911	VBN	denotes	stained
T2381	8912-8916	IN	denotes	with
T2382	8917-8918	CD	denotes	1
T2384	8918-8919	,	denotes	,
T2385	8919-8920	CD	denotes	1
T2386	8920-8921	SYM	denotes	'
T2387	8921-8922	HYPH	denotes	-
T2383	8922-8933	NN	denotes	dioctadecyl
T2388	8933-8934	HYPH	denotes	-
T2389	8934-8935	CD	denotes	3
T2390	8935-8936	,	denotes	,
T2391	8936-8937	CD	denotes	3
T2393	8937-8938	,	denotes	,
T2394	8938-8939	CD	denotes	3
T2395	8939-8940	SYM	denotes	'
T2396	8940-8941	,	denotes	,
T2397	8941-8942	CD	denotes	3
T2398	8942-8943	SYM	denotes	'
T2399	8943-8944	HYPH	denotes	-
T2400	8944-8971	JJ	denotes	tetramethylindocarbocyanine
T2392	8972-8983	NN	denotes	perchlorate
T2401	8984-8985	-LRB-	denotes	(
T2403	8985-8986	``	denotes	'
T2404	8986-8989	NN	denotes	DiI
T2405	8989-8990	''	denotes	'
T2406	8990-8991	:	denotes	;
T2407	8991-8997	NN	denotes	DiIC18
T2408	8997-8998	:	denotes	;
T2409	8999-9008	NNP	denotes	Molecular
T2402	9009-9015	NNP	denotes	Probes
T2410	9015-9016	-RRB-	denotes	)
T2411	9017-9019	IN	denotes	as
T2413	9020-9030	RB	denotes	previously
T2412	9031-9040	VBN	denotes	described
T2414	9041-9042	-LRB-	denotes	[
T2415	9042-9044	CD	denotes	24
T2416	9044-9045	-RRB-	denotes	]
T2417	9045-9046	.	denotes	.
T2418	9046-9301	sentence	denotes	Sections were then washed several times with Tris buffered saline, and nucleic acids were stained by incubating sections in TBS containing 1 μM SYTOX Green (Molecular Probes) for 10 min at RT followed by several washes with TBS prior to confocal imaging.
T2419	9047-9055	NNS	denotes	Sections
T2421	9056-9060	VBD	denotes	were
T2422	9061-9065	RB	denotes	then
T2420	9066-9072	VBN	denotes	washed
T2423	9073-9080	JJ	denotes	several
T2424	9081-9086	NNS	denotes	times
T2425	9087-9091	IN	denotes	with
T2426	9092-9096	NNP	denotes	Tris
T2427	9097-9105	VBN	denotes	buffered
T2428	9106-9112	NN	denotes	saline
T2429	9112-9114	,	denotes	,
T2430	9114-9117	CC	denotes	and
T2431	9118-9125	JJ	denotes	nucleic
T2432	9126-9131	NNS	denotes	acids
T2434	9132-9136	VBD	denotes	were
T2433	9137-9144	VBN	denotes	stained
T2435	9145-9147	IN	denotes	by
T2436	9148-9158	VBG	denotes	incubating
T2437	9159-9167	NNS	denotes	sections
T2438	9168-9170	IN	denotes	in
T2439	9171-9174	NN	denotes	TBS
T2440	9175-9185	VBG	denotes	containing
T2441	9186-9187	CD	denotes	1
T2442	9188-9190	NN	denotes	μM
T2444	9191-9196	NN	denotes	SYTOX
T2443	9197-9202	NN	denotes	Green
T2445	9203-9204	-LRB-	denotes	(
T2447	9204-9213	JJ	denotes	Molecular
T2446	9214-9220	NNP	denotes	Probes
T2448	9220-9221	-RRB-	denotes	)
T2449	9222-9225	IN	denotes	for
T2450	9226-9228	CD	denotes	10
T2451	9229-9232	NN	denotes	min
T2452	9233-9235	IN	denotes	at
T2453	9236-9238	NN	denotes	RT
T2454	9239-9247	VBN	denotes	followed
T2455	9248-9250	IN	denotes	by
T2456	9251-9258	JJ	denotes	several
T2457	9259-9265	NNS	denotes	washes
T2458	9266-9270	IN	denotes	with
T2459	9271-9274	NN	denotes	TBS
T2460	9275-9280	RB	denotes	prior
T2461	9281-9283	IN	denotes	to
T2462	9284-9292	JJ	denotes	confocal
T2463	9293-9300	NN	denotes	imaging
T2464	9300-9301	.	denotes	.
T2465	9301-9704	sentence	denotes	Lens sections were viewed on a Zeiss laser scanning confocal microscope model LSM 410 equipped with an Axiovert 100 inverted microscope, an Argon-Krypton 488/568/647 laser, a KP line selection filter, a FT 488/568 Dichroic beam splitter, a FT 560 Dichroic beam splitter, a LP 590 emission filter for viewing DiI, a BP 515–540 emission filter for viewing SYTOX green, and the software package LSM 3.993.
T2466	9302-9306	NNP	denotes	Lens
T2467	9307-9315	NNS	denotes	sections
T2469	9316-9320	VBD	denotes	were
T2468	9321-9327	VBN	denotes	viewed
T2470	9328-9330	IN	denotes	on
T2471	9331-9332	DT	denotes	a
T2473	9333-9338	NNP	denotes	Zeiss
T2474	9339-9344	NN	denotes	laser
T2475	9345-9353	NN	denotes	scanning
T2476	9354-9362	JJ	denotes	confocal
T2472	9363-9373	NN	denotes	microscope
T2477	9374-9379	NN	denotes	model
T2478	9380-9383	NN	denotes	LSM
T2479	9384-9387	CD	denotes	410
T2480	9388-9396	VBN	denotes	equipped
T2481	9397-9401	IN	denotes	with
T2482	9402-9404	DT	denotes	an
T2484	9405-9413	JJ	denotes	Axiovert
T2485	9414-9417	CD	denotes	100
T2486	9418-9426	JJ	denotes	inverted
T2483	9427-9437	NN	denotes	microscope
T2487	9437-9439	,	denotes	,
T2488	9439-9441	DT	denotes	an
T2490	9442-9447	JJ	denotes	Argon
T2492	9447-9448	HYPH	denotes	-
T2491	9448-9455	JJ	denotes	Krypton
T2493	9456-9459	CD	denotes	488
T2494	9459-9460	HYPH	denotes	/
T2495	9460-9463	CD	denotes	568
T2496	9463-9464	HYPH	denotes	/
T2497	9464-9467	CD	denotes	647
T2489	9468-9473	NN	denotes	laser
T2498	9473-9475	,	denotes	,
T2499	9475-9476	DT	denotes	a
T2501	9477-9479	NN	denotes	KP
T2502	9480-9484	NN	denotes	line
T2503	9485-9494	NN	denotes	selection
T2500	9495-9501	NN	denotes	filter
T2504	9501-9503	,	denotes	,
T2505	9503-9504	DT	denotes	a
T2507	9505-9507	NN	denotes	FT
T2508	9508-9511	CD	denotes	488
T2509	9511-9512	HYPH	denotes	/
T2510	9512-9515	CD	denotes	568
T2511	9516-9524	JJ	denotes	Dichroic
T2512	9525-9529	NN	denotes	beam
T2506	9530-9538	NN	denotes	splitter
T2513	9538-9540	,	denotes	,
T2514	9540-9541	DT	denotes	a
T2516	9542-9544	NN	denotes	FT
T2517	9545-9548	CD	denotes	560
T2518	9549-9557	JJ	denotes	Dichroic
T2519	9558-9562	NN	denotes	beam
T2515	9563-9571	NN	denotes	splitter
T2520	9571-9573	,	denotes	,
T2521	9573-9574	DT	denotes	a
T2523	9575-9577	NN	denotes	LP
T2524	9578-9581	CD	denotes	590
T2525	9582-9590	NN	denotes	emission
T2522	9591-9597	NN	denotes	filter
T2526	9598-9601	IN	denotes	for
T2527	9602-9609	VBG	denotes	viewing
T2528	9610-9613	NNP	denotes	DiI
T2529	9613-9615	,	denotes	,
T2530	9615-9616	DT	denotes	a
T2532	9617-9619	NN	denotes	BP
T2533	9620-9623	CD	denotes	515
T2534	9623-9624	SYM	denotes	–
T2535	9624-9627	CD	denotes	540
T2536	9628-9636	NN	denotes	emission
T2531	9637-9643	NN	denotes	filter
T2537	9644-9647	IN	denotes	for
T2538	9648-9655	VBG	denotes	viewing
T2539	9656-9661	NN	denotes	SYTOX
T2540	9662-9667	NN	denotes	green
T2541	9667-9669	,	denotes	,
T2542	9669-9672	CC	denotes	and
T2543	9673-9676	DT	denotes	the
T2545	9677-9685	NN	denotes	software
T2544	9686-9693	NN	denotes	package
T2546	9694-9697	NN	denotes	LSM
T2547	9698-9703	CD	denotes	3.993
T2548	9703-9704	.	denotes	.
T2588	9706-9714	NN	denotes	Scanning
T2590	9715-9723	NN	denotes	electron
T2589	9724-9734	NN	denotes	microscopy
T2591	9734-9910	sentence	denotes	Lenses bisected along the optical axes were immersion fixed in 2% (w/v) paraformaldehyde and 2% glutaraldehyde (v/v) in 0.1 M sodium cacodylate buffer pH 7.2 at RT for 24 hrs.
T2592	9735-9741	NNS	denotes	Lenses
T2594	9742-9750	VBN	denotes	bisected
T2595	9751-9756	IN	denotes	along
T2596	9757-9760	DT	denotes	the
T2598	9761-9768	JJ	denotes	optical
T2597	9769-9773	NNS	denotes	axes
T2599	9774-9778	VBD	denotes	were
T2600	9779-9788	NN	denotes	immersion
T2593	9789-9794	VBN	denotes	fixed
T2601	9795-9797	IN	denotes	in
T2602	9798-9799	CD	denotes	2
T2603	9799-9800	NN	denotes	%
T2605	9801-9802	-LRB-	denotes	(
T2606	9802-9803	NN	denotes	w
T2607	9803-9804	SYM	denotes	/
T2608	9804-9805	NN	denotes	v
T2609	9805-9806	-RRB-	denotes	)
T2604	9807-9823	NN	denotes	paraformaldehyde
T2610	9824-9827	CC	denotes	and
T2611	9828-9829	CD	denotes	2
T2612	9829-9830	NN	denotes	%
T2613	9831-9845	NN	denotes	glutaraldehyde
T2614	9846-9847	-LRB-	denotes	(
T2615	9847-9848	NN	denotes	v
T2616	9848-9849	SYM	denotes	/
T2617	9849-9850	NN	denotes	v
T2618	9850-9851	-RRB-	denotes	)
T2619	9852-9854	IN	denotes	in
T2620	9855-9858	CD	denotes	0.1
T2621	9859-9860	NN	denotes	M
T2623	9861-9867	NN	denotes	sodium
T2622	9868-9878	NN	denotes	cacodylate
T2624	9879-9885	NN	denotes	buffer
T2625	9886-9888	NN	denotes	pH
T2626	9889-9892	CD	denotes	7.2
T2627	9893-9895	IN	denotes	at
T2628	9896-9898	NN	denotes	RT
T2629	9899-9902	IN	denotes	for
T2630	9903-9905	CD	denotes	24
T2631	9906-9909	NNS	denotes	hrs
T2632	9909-9910	.	denotes	.
T2633	9910-10037	sentence	denotes	Samples were then washed several times with distilled water and postfixed in 1% (v/v) aqueous osmium tetroxide at RT for 1 hr.
T2634	9911-9918	NNS	denotes	Samples
T2636	9919-9923	VBD	denotes	were
T2637	9924-9928	RB	denotes	then
T2635	9929-9935	VBN	denotes	washed
T2638	9936-9943	JJ	denotes	several
T2639	9944-9949	NNS	denotes	times
T2640	9950-9954	IN	denotes	with
T2641	9955-9964	JJ	denotes	distilled
T2642	9965-9970	NN	denotes	water
T2643	9971-9974	CC	denotes	and
T2644	9975-9984	VBN	denotes	postfixed
T2645	9985-9987	IN	denotes	in
T2646	9988-9989	CD	denotes	1
T2647	9989-9990	NN	denotes	%
T2649	9991-9992	-LRB-	denotes	(
T2650	9992-9993	NN	denotes	v
T2651	9993-9994	SYM	denotes	/
T2652	9994-9995	NN	denotes	v
T2653	9995-9996	-RRB-	denotes	)
T2654	9997-10004	JJ	denotes	aqueous
T2655	10005-10011	NN	denotes	osmium
T2648	10012-10021	NN	denotes	tetroxide
T2656	10022-10024	IN	denotes	at
T2657	10025-10027	NN	denotes	RT
T2658	10028-10031	IN	denotes	for
T2659	10032-10033	CD	denotes	1
T2660	10034-10036	NN	denotes	hr
T2661	10036-10037	.	denotes	.
T2662	10037-10111	sentence	denotes	Lens halves were dehydrated in an ascending ethanol series from 50–100 %.
T2663	10038-10042	NNP	denotes	Lens
T2664	10043-10049	NNS	denotes	halves
T2666	10050-10054	VBD	denotes	were
T2665	10055-10065	VBN	denotes	dehydrated
T2667	10066-10068	IN	denotes	in
T2668	10069-10071	DT	denotes	an
T2670	10072-10081	VBG	denotes	ascending
T2671	10082-10089	NN	denotes	ethanol
T2669	10090-10096	NN	denotes	series
T2672	10097-10101	IN	denotes	from
T2673	10102-10104	CD	denotes	50
T2675	10104-10105	SYM	denotes	–
T2674	10105-10108	CD	denotes	100
T2676	10109-10110	NN	denotes	%
T2677	10110-10111	.	denotes	.
T2678	10111-10258	sentence	denotes	Once lens halves were in 100% ethanol, they were critical point dried in carbon dioxide in a Samdri-790B (Tousimis Research Corp., Rockville, MD).
T2679	10112-10116	IN	denotes	Once
T2681	10117-10121	NN	denotes	lens
T2682	10122-10128	NNS	denotes	halves
T2680	10129-10133	VBD	denotes	were
T2684	10134-10136	IN	denotes	in
T2685	10137-10140	CD	denotes	100
T2686	10140-10141	NN	denotes	%
T2687	10142-10149	NN	denotes	ethanol
T2688	10149-10151	,	denotes	,
T2689	10151-10155	PRP	denotes	they
T2690	10156-10160	VBD	denotes	were
T2691	10161-10169	JJ	denotes	critical
T2692	10170-10175	NN	denotes	point
T2683	10176-10181	VBN	denotes	dried
T2693	10182-10184	IN	denotes	in
T2694	10185-10191	NN	denotes	carbon
T2695	10192-10199	NN	denotes	dioxide
T2696	10200-10202	IN	denotes	in
T2697	10203-10204	DT	denotes	a
T2699	10205-10211	NN	denotes	Samdri
T2700	10211-10212	HYPH	denotes	-
T2698	10212-10216	NN	denotes	790B
T2701	10217-10218	-LRB-	denotes	(
T2703	10218-10226	NNP	denotes	Tousimis
T2704	10227-10235	NNP	denotes	Research
T2702	10236-10241	NNP	denotes	Corp.
T2705	10241-10243	,	denotes	,
T2706	10243-10252	NNP	denotes	Rockville
T2707	10252-10254	,	denotes	,
T2708	10254-10256	NNP	denotes	MD
T2709	10256-10257	-RRB-	denotes	)
T2710	10257-10258	.	denotes	.
T2711	10258-10346	sentence	denotes	Critical point dried lens halves were secured on aluminum stubs with double sided tape.
T2712	10259-10267	JJ	denotes	Critical
T2713	10268-10273	NN	denotes	point
T2714	10274-10279	VBN	denotes	dried
T2716	10280-10284	NN	denotes	lens
T2715	10285-10291	NNS	denotes	halves
T2718	10292-10296	VBD	denotes	were
T2717	10297-10304	VBN	denotes	secured
T2719	10305-10307	IN	denotes	on
T2720	10308-10316	NN	denotes	aluminum
T2721	10317-10322	NNS	denotes	stubs
T2722	10323-10327	IN	denotes	with
T2723	10328-10334	NN	denotes	double
T2724	10335-10340	VBN	denotes	sided
T2725	10341-10345	NN	denotes	tape
T2726	10345-10346	.	denotes	.
T2727	10346-10486	sentence	denotes	Mounted specimens were then sputter coated with gold and viewed on a Hitachi S-3500N scanning electron microscope (Tokyo, Japan) at 1–5 kV.
T2728	10347-10354	VBN	denotes	Mounted
T2729	10355-10364	NNS	denotes	specimens
T2731	10365-10369	VBD	denotes	were
T2732	10370-10374	RB	denotes	then
T2733	10375-10382	NN	denotes	sputter
T2730	10383-10389	VBN	denotes	coated
T2734	10390-10394	IN	denotes	with
T2735	10395-10399	NN	denotes	gold
T2736	10400-10403	CC	denotes	and
T2737	10404-10410	VBN	denotes	viewed
T2738	10411-10413	IN	denotes	on
T2739	10414-10415	DT	denotes	a
T2741	10416-10423	NNP	denotes	Hitachi
T2743	10424-10425	NN	denotes	S
T2744	10425-10426	HYPH	denotes	-
T2742	10426-10431	NN	denotes	3500N
T2745	10432-10440	NN	denotes	scanning
T2746	10441-10449	NN	denotes	electron
T2740	10450-10460	NN	denotes	microscope
T2747	10461-10462	-LRB-	denotes	(
T2748	10462-10467	NNP	denotes	Tokyo
T2749	10467-10469	,	denotes	,
T2750	10469-10474	NNP	denotes	Japan
T2751	10474-10475	-RRB-	denotes	)
T2752	10476-10478	IN	denotes	at
T2753	10479-10480	CD	denotes	1
T2755	10480-10481	SYM	denotes	–
T2754	10481-10482	CD	denotes	5
T2756	10483-10485	NN	denotes	kV
T2757	10485-10486	.	denotes	.
T2758	10486-10640	sentence	denotes	This microscope is equipped with Hitachi's patented "Dual-Bias" which allows extremely high emission currents at acceleration voltages of 5 kV and lower.
T2759	10487-10491	DT	denotes	This
T2760	10492-10502	NN	denotes	microscope
T2762	10503-10505	VBZ	denotes	is
T2761	10506-10514	VBN	denotes	equipped
T2763	10515-10519	IN	denotes	with
T2764	10520-10527	NNP	denotes	Hitachi
T2766	10527-10529	POS	denotes	's
T2767	10530-10538	VBN	denotes	patented
T2768	10539-10540	``	denotes	"
T2769	10540-10544	NNP	denotes	Dual
T2770	10544-10545	HYPH	denotes	-
T2765	10545-10549	NNP	denotes	Bias
T2771	10549-10550	''	denotes	"
T2772	10551-10556	WDT	denotes	which
T2773	10557-10563	VBZ	denotes	allows
T2774	10564-10573	RB	denotes	extremely
T2775	10574-10578	JJ	denotes	high
T2777	10579-10587	NN	denotes	emission
T2776	10588-10596	NNS	denotes	currents
T2778	10597-10599	IN	denotes	at
T2779	10600-10612	NN	denotes	acceleration
T2780	10613-10621	NNS	denotes	voltages
T2781	10622-10624	IN	denotes	of
T2782	10625-10626	CD	denotes	5
T2783	10627-10629	NN	denotes	kV
T2784	10630-10633	CC	denotes	and
T2785	10634-10639	JJR	denotes	lower
T2786	10639-10640	.	denotes	.
T3299	10702-10704	,	denotes	,
T3300	10704-10707	CC	denotes	and
T3301	10708-10713	JJ	denotes	gross
T3303	10714-10724	JJ	denotes	lenticular
T3302	10725-10735	NN	denotes	appearance
T3304	10735-10737	,	denotes	,
T3305	10737-10741	VBD	denotes	were
T3296	10742-10750	VBN	denotes	recorded
T3306	10751-10752	-LRB-	denotes	(
T3308	10752-10757	JJ	denotes	Table
T3307	10758-10759	CD	denotes	1
T3309	10759-10760	-RRB-	denotes	)
T3310	10760-10761	.	denotes	.
T3311	10761-10932	sentence	denotes	AlphaA/BKO lenses at 5 wks of age were significantly smaller (p < 0.05), both equatorially and axially, than 5 wk old wild type lenses and remain smaller throughout life.
T3312	10762-10768	NN	denotes	AlphaA
T3314	10768-10769	HYPH	denotes	/
T3313	10769-10772	NN	denotes	BKO
T3315	10773-10779	NNS	denotes	lenses
T3317	10780-10782	IN	denotes	at
T3318	10783-10784	CD	denotes	5
T3319	10785-10788	NNS	denotes	wks
T3320	10789-10791	IN	denotes	of
T3321	10792-10795	NN	denotes	age
T3316	10796-10800	VBD	denotes	were
T3322	10801-10814	RB	denotes	significantly
T3323	10815-10822	JJR	denotes	smaller
T3324	10823-10824	-LRB-	denotes	(
T3326	10824-10825	NN	denotes	p
T3327	10826-10827	SYM	denotes	<
T3325	10828-10832	CD	denotes	0.05
T3328	10832-10833	-RRB-	denotes	)
T3329	10833-10835	,	denotes	,
T3330	10835-10839	CC	denotes	both
T3331	10840-10852	RB	denotes	equatorially
T3332	10853-10856	CC	denotes	and
T3333	10857-10864	RB	denotes	axially
T3334	10864-10866	,	denotes	,
T3335	10866-10870	IN	denotes	than
T3336	10871-10872	CD	denotes	5
T3337	10873-10875	NN	denotes	wk
T3338	10876-10879	JJ	denotes	old
T3340	10880-10884	JJ	denotes	wild
T3341	10885-10889	NN	denotes	type
T3339	10890-10896	NNS	denotes	lenses
T3342	10897-10900	CC	denotes	and
T3343	10901-10907	VBP	denotes	remain
T3344	10908-10915	JJR	denotes	smaller
T3345	10916-10926	IN	denotes	throughout
T3346	10927-10931	NN	denotes	life
T3347	10931-10932	.	denotes	.
T3348	10932-11076	sentence	denotes	Fifty-four wk old alphaA/BKO lenses were much smaller than similarly aged normal lenses, and were similar in size to 5 wk old wild type lenses.
T3349	10933-10938	CD	denotes	Fifty
T3351	10938-10939	HYPH	denotes	-
T3350	10939-10943	CD	denotes	four
T3352	10944-10946	NN	denotes	wk
T3353	10947-10950	JJ	denotes	old
T3355	10951-10957	NN	denotes	alphaA
T3357	10957-10958	HYPH	denotes	/
T3356	10958-10961	NN	denotes	BKO
T3354	10962-10968	NNS	denotes	lenses
T3358	10969-10973	VBD	denotes	were
T3359	10974-10978	RB	denotes	much
T3360	10979-10986	JJR	denotes	smaller
T3361	10987-10991	IN	denotes	than
T3362	10992-11001	RB	denotes	similarly
T3363	11002-11006	VBN	denotes	aged
T3365	11007-11013	JJ	denotes	normal
T3364	11014-11020	NNS	denotes	lenses
T3366	11020-11022	,	denotes	,
T3367	11022-11025	CC	denotes	and
T3368	11026-11030	VBD	denotes	were
T3369	11031-11038	JJ	denotes	similar
T3370	11039-11041	IN	denotes	in
T3371	11042-11046	NN	denotes	size
T3372	11047-11049	IN	denotes	to
T3373	11050-11051	CD	denotes	5
T3374	11052-11054	NN	denotes	wk
T3375	11055-11058	JJ	denotes	old
T3377	11059-11063	JJ	denotes	wild
T3378	11064-11068	NN	denotes	type
T3376	11069-11075	NNS	denotes	lenses
T3379	11075-11076	.	denotes	.
T3380	11076-11228	sentence	denotes	Both the 5 wk old wild type lenses and the 54 wk old alphaA/BKO lenses were significantly smaller (p < 0.01) than the 46 or 72 wk old wild type lenses.
T3381	11077-11081	CC	denotes	Both
T3383	11082-11085	DT	denotes	the
T3384	11086-11087	CD	denotes	5
T3385	11088-11090	NN	denotes	wk
T3386	11091-11094	JJ	denotes	old
T3387	11095-11099	JJ	denotes	wild
T3388	11100-11104	NN	denotes	type
T3382	11105-11111	NNS	denotes	lenses
T3390	11112-11115	CC	denotes	and
T3391	11116-11119	DT	denotes	the
T3393	11120-11122	CD	denotes	54
T3394	11123-11125	NN	denotes	wk
T3395	11126-11129	JJ	denotes	old
T3396	11130-11136	NN	denotes	alphaA
T3398	11136-11137	HYPH	denotes	/
T3397	11137-11140	NN	denotes	BKO
T3392	11141-11147	NNS	denotes	lenses
T3389	11148-11152	VBD	denotes	were
T3399	11153-11166	RB	denotes	significantly
T3400	11167-11174	JJR	denotes	smaller
T3401	11175-11176	-LRB-	denotes	(
T3403	11176-11177	NN	denotes	p
T3404	11178-11179	SYM	denotes	<
T3402	11180-11184	CD	denotes	0.01
T3405	11184-11185	-RRB-	denotes	)
T3406	11186-11190	IN	denotes	than
T3407	11191-11194	DT	denotes	the
T3409	11195-11197	CD	denotes	46
T3411	11198-11200	CC	denotes	or
T3412	11201-11203	CD	denotes	72
T3410	11204-11206	NN	denotes	wk
T3413	11207-11210	JJ	denotes	old
T3414	11211-11215	JJ	denotes	wild
T3415	11216-11220	NN	denotes	type
T3408	11221-11227	NNS	denotes	lenses
T3416	11227-11228	.	denotes	.
T3417	11228-11430	sentence	denotes	AlphaA/BKO lenses at 5 wks of age exhibited vacuoles at the equatorial region (Figure 1A see arrows) and an area of slight light scatter at the posterior subcapsular regions (Figure 1B see arrowheads).
T3418	11229-11235	NN	denotes	AlphaA
T3420	11235-11236	HYPH	denotes	/
T3419	11236-11239	NN	denotes	BKO
T3421	11240-11246	NNS	denotes	lenses
T3423	11247-11249	IN	denotes	at
T3424	11250-11251	CD	denotes	5
T3425	11252-11255	NNS	denotes	wks
T3426	11256-11258	IN	denotes	of
T3427	11259-11262	NN	denotes	age
T3422	11263-11272	VBD	denotes	exhibited
T3428	11273-11281	NNS	denotes	vacuoles
T3429	11282-11284	IN	denotes	at
T3430	11285-11288	DT	denotes	the
T3432	11289-11299	JJ	denotes	equatorial
T3431	11300-11306	NN	denotes	region
T3433	11307-11308	-LRB-	denotes	(
T3435	11308-11314	NN	denotes	Figure
T3434	11315-11317	NN	denotes	1A
T3436	11318-11321	VB	denotes	see
T3437	11322-11328	NNS	denotes	arrows
T3438	11328-11329	-RRB-	denotes	)
T3439	11330-11333	CC	denotes	and
T3440	11334-11336	DT	denotes	an
T3441	11337-11341	NN	denotes	area
T3442	11342-11344	IN	denotes	of
T3443	11345-11351	JJ	denotes	slight
T3445	11352-11357	JJ	denotes	light
T3444	11358-11365	NN	denotes	scatter
T3446	11366-11368	IN	denotes	at
T3447	11369-11372	DT	denotes	the
T3449	11373-11382	JJ	denotes	posterior
T3450	11383-11394	JJ	denotes	subcapsular
T3448	11395-11402	NNS	denotes	regions
T3451	11403-11404	-LRB-	denotes	(
T3453	11404-11410	NN	denotes	Figure
T3452	11411-11413	NN	denotes	1B
T3454	11414-11417	VB	denotes	see
T3455	11418-11428	NNS	denotes	arrowheads
T3456	11428-11429	-RRB-	denotes	)
T3457	11429-11430	.	denotes	.
T3458	11430-11526	sentence	denotes	By 54 wks of age, alphaA/BKO lenses exhibited dense cortical and nuclear cataracts (Figure 1C).
T3459	11431-11433	IN	denotes	By
T3461	11434-11436	CD	denotes	54
T3462	11437-11440	NNS	denotes	wks
T3463	11441-11443	IN	denotes	of
T3464	11444-11447	NN	denotes	age
T3465	11447-11449	,	denotes	,
T3466	11449-11455	NN	denotes	alphaA
T3468	11455-11456	HYPH	denotes	/
T3467	11456-11459	NN	denotes	BKO
T3469	11460-11466	NNS	denotes	lenses
T3460	11467-11476	VBD	denotes	exhibited
T3470	11477-11482	JJ	denotes	dense
T3472	11483-11491	JJ	denotes	cortical
T3473	11492-11495	CC	denotes	and
T3474	11496-11503	JJ	denotes	nuclear
T3471	11504-11513	NNS	denotes	cataracts
T3475	11514-11515	-LRB-	denotes	(
T3477	11515-11521	NN	denotes	Figure
T3476	11522-11524	NN	denotes	1C
T3478	11524-11525	-RRB-	denotes	)
T3479	11525-11526	.	denotes	.
T3480	11526-11603	sentence	denotes	No posterior sutures were observed in any of the alphaA/BKO lenses examined.
T3481	11527-11529	DT	denotes	No
T3483	11530-11539	JJ	denotes	posterior
T3482	11540-11547	NNS	denotes	sutures
T3485	11548-11552	VBD	denotes	were
T3484	11553-11561	VBN	denotes	observed
T3486	11562-11564	IN	denotes	in
T3487	11565-11568	DT	denotes	any
T3488	11569-11571	IN	denotes	of
T3489	11572-11575	DT	denotes	the
T3491	11576-11582	NN	denotes	alphaA
T3493	11582-11583	HYPH	denotes	/
T3492	11583-11586	NN	denotes	BKO
T3490	11587-11593	NNS	denotes	lenses
T3494	11594-11602	VBN	denotes	examined
T3495	11602-11603	.	denotes	.
T3496	11603-11729	sentence	denotes	All wild type lenses were transparent upon removal from the eye (Figure 1D,1E and 1F) and had well defined posterior sutures.
T3497	11604-11607	DT	denotes	All
T3499	11608-11612	JJ	denotes	wild
T3500	11613-11617	NN	denotes	type
T3498	11618-11624	NNS	denotes	lenses
T3501	11625-11629	VBD	denotes	were
T3502	11630-11641	JJ	denotes	transparent
T3503	11642-11646	IN	denotes	upon
T3504	11647-11654	NN	denotes	removal
T3505	11655-11659	IN	denotes	from
T3506	11660-11663	DT	denotes	the
T3507	11664-11667	NN	denotes	eye
T3508	11668-11669	-LRB-	denotes	(
T3510	11669-11675	NN	denotes	Figure
T3509	11676-11678	NN	denotes	1D
T3511	11678-11679	,	denotes	,
T3512	11679-11681	NN	denotes	1E
T3513	11682-11685	CC	denotes	and
T3514	11686-11688	NN	denotes	1F
T3515	11688-11689	-RRB-	denotes	)
T3516	11690-11693	CC	denotes	and
T3517	11694-11697	VBD	denotes	had
T3518	11698-11702	RB	denotes	well
T3519	11703-11710	VBN	denotes	defined
T3521	11711-11720	JJ	denotes	posterior
T3520	11721-11728	NNS	denotes	sutures
T3522	11728-11729	.	denotes	.
T3523	11729-11730	sentence	denotes
T8033	11740-11745	NN	denotes	Light
T8034	11746-11757	NNS	denotes	micrographs
T8035	11758-11760	IN	denotes	of
T8036	11761-11767	NNS	denotes	lenses
T8037	11768-11777	VBN	denotes	dissected
T8038	11778-11782	IN	denotes	from
T8039	11783-11788	VBN	denotes	fixed
T8040	11789-11793	NNS	denotes	eyes
T8041	11794-11796	IN	denotes	of
T8042	11797-11803	NN	denotes	alphaA
T8044	11803-11804	HYPH	denotes	/
T8043	11804-11807	NN	denotes	BKO
T8045	11808-11809	-LRB-	denotes	(
T8046	11809-11810	NN	denotes	A
T8047	11810-11811	SYM	denotes	-
T8048	11811-11812	NN	denotes	C
T8049	11812-11813	-RRB-	denotes	)
T8050	11814-11817	CC	denotes	and
T8051	11818-11822	JJ	denotes	wild
T8052	11823-11827	NN	denotes	type
T8054	11828-11829	-LRB-	denotes	(
T8055	11829-11830	NN	denotes	D
T8056	11830-11831	SYM	denotes	-
T8057	11831-11832	NN	denotes	F
T8058	11832-11833	-RRB-	denotes	)
T8053	11834-11838	NNS	denotes	mice
T8059	11838-11839	.	denotes	.
T8060	11839-11947	sentence	denotes	All micrographs were generated using a 4 × /0.10 objective on a Zeiss LSM 410 using transmitted light mode.
T8061	11840-11843	DT	denotes	All
T8062	11844-11855	NNS	denotes	micrographs
T8064	11856-11860	VBD	denotes	were
T8063	11861-11870	VBN	denotes	generated
T8065	11871-11876	VBG	denotes	using
T8066	11877-11878	DT	denotes	a
T8068	11879-11880	CD	denotes	4
T8069	11881-11882	SYM	denotes	×
T8071	11883-11884	SYM	denotes	/
T8070	11884-11888	CD	denotes	0.10
T8067	11889-11898	NN	denotes	objective
T8072	11899-11901	IN	denotes	on
T8073	11902-11903	DT	denotes	a
T8075	11904-11909	NNP	denotes	Zeiss
T8074	11910-11913	NNP	denotes	LSM
T8076	11914-11917	CD	denotes	410
T8077	11918-11923	VBG	denotes	using
T8078	11924-11935	VBN	denotes	transmitted
T8079	11936-11941	NN	denotes	light
T8080	11942-11946	NN	denotes	mode
T8081	11946-11947	.	denotes	.
T8082	11947-12020	sentence	denotes	Images in (A&B) were enlarged compared to images (C-F) (see scale bars).
T8083	11948-11954	NNS	denotes	Images
T8085	11955-11957	IN	denotes	in
T8086	11958-11959	-LRB-	denotes	(
T8087	11959-11960	NN	denotes	A
T8089	11960-11961	SYM	denotes	&
T8088	11961-11962	NN	denotes	B
T8090	11962-11963	-RRB-	denotes	)
T8084	11964-11968	VBD	denotes	were
T8091	11969-11977	JJ	denotes	enlarged
T8092	11978-11986	VBN	denotes	compared
T8093	11987-11989	IN	denotes	to
T8094	11990-11996	NNS	denotes	images
T8096	11997-11998	-LRB-	denotes	(
T8095	11998-11999	NN	denotes	C
T8097	11999-12000	SYM	denotes	-
T8098	12000-12001	NN	denotes	F
T8099	12001-12002	-RRB-	denotes	)
T8100	12003-12004	-LRB-	denotes	(
T8101	12004-12007	VB	denotes	see
T8102	12008-12013	NN	denotes	scale
T8103	12014-12018	NNS	denotes	bars
T8104	12018-12019	-RRB-	denotes	)
T8105	12019-12020	.	denotes	.
T8106	12020-12175	sentence	denotes	For all micrographs except (B) the objective focal point was set at the equatorial plane of the lens, while (B) was set at the posterior pole of the lens.
T8107	12021-12024	IN	denotes	For
T8109	12025-12028	DT	denotes	all
T8110	12029-12040	NNS	denotes	micrographs
T8111	12041-12047	IN	denotes	except
T8112	12048-12049	-LRB-	denotes	(
T8113	12049-12050	NN	denotes	B
T8114	12050-12051	-RRB-	denotes	)
T8115	12052-12055	DT	denotes	the
T8117	12056-12065	JJ	denotes	objective
T8118	12066-12071	JJ	denotes	focal
T8116	12072-12077	NN	denotes	point
T8119	12078-12081	VBD	denotes	was
T8108	12082-12085	VBN	denotes	set
T8120	12086-12088	IN	denotes	at
T8121	12089-12092	DT	denotes	the
T8123	12093-12103	JJ	denotes	equatorial
T8122	12104-12109	NN	denotes	plane
T8124	12110-12112	IN	denotes	of
T8125	12113-12116	DT	denotes	the
T8126	12117-12121	NN	denotes	lens
T8127	12121-12123	,	denotes	,
T8128	12123-12128	IN	denotes	while
T8130	12129-12130	-LRB-	denotes	(
T8131	12130-12131	NN	denotes	B
T8132	12131-12132	-RRB-	denotes	)
T8133	12133-12136	VBD	denotes	was
T8129	12137-12140	VBN	denotes	set
T8134	12141-12143	IN	denotes	at
T8135	12144-12147	DT	denotes	the
T8137	12148-12157	JJ	denotes	posterior
T8136	12158-12162	NN	denotes	pole
T8138	12163-12165	IN	denotes	of
T8139	12166-12169	DT	denotes	the
T8140	12170-12174	NN	denotes	lens
T8141	12174-12175	.	denotes	.
T8142	12175-12323	sentence	denotes	(A&B) Representative micrographs of lenses from 5 wk alphaA/BKO mice, showing changes in the equatorial (A) and posterior sub capsular (B) regions.
T8143	12176-12177	-LRB-	denotes	(
T8144	12177-12178	LS	denotes	A
T8146	12178-12179	SYM	denotes	&
T8147	12179-12180	LS	denotes	B
T8148	12180-12181	-RRB-	denotes	)
T8149	12182-12196	JJ	denotes	Representative
T8145	12197-12208	NNS	denotes	micrographs
T8150	12209-12211	IN	denotes	of
T8151	12212-12218	NNS	denotes	lenses
T8152	12219-12223	IN	denotes	from
T8153	12224-12225	CD	denotes	5
T8154	12226-12228	NN	denotes	wk
T8156	12229-12235	NN	denotes	alphaA
T8158	12235-12236	HYPH	denotes	/
T8157	12236-12239	NN	denotes	BKO
T8155	12240-12244	NNS	denotes	mice
T8159	12244-12246	,	denotes	,
T8160	12246-12253	VBG	denotes	showing
T8161	12254-12261	NNS	denotes	changes
T8162	12262-12264	IN	denotes	in
T8163	12265-12268	DT	denotes	the
T8165	12269-12279	JJ	denotes	equatorial
T8166	12280-12281	-LRB-	denotes	(
T8167	12281-12282	NN	denotes	A
T8168	12282-12283	-RRB-	denotes	)
T8169	12284-12287	CC	denotes	and
T8170	12288-12297	JJ	denotes	posterior
T8171	12298-12301	AFX	denotes	sub
T8172	12302-12310	JJ	denotes	capsular
T8173	12311-12312	-LRB-	denotes	(
T8174	12312-12313	NN	denotes	B
T8175	12313-12314	-RRB-	denotes	)
T8164	12315-12322	NNS	denotes	regions
T8176	12322-12323	.	denotes	.
T8177	12323-12419	sentence	denotes	(C) Representative micrograph of 54 wk alphaA/BKO mouse lens showing dense whole lens cataract.
T8178	12324-12325	-LRB-	denotes	(
T8179	12325-12326	LS	denotes	C
T8181	12326-12327	-RRB-	denotes	)
T8182	12328-12342	JJ	denotes	Representative
T8180	12343-12353	NN	denotes	micrograph
T8183	12354-12356	IN	denotes	of
T8184	12357-12359	CD	denotes	54
T8185	12360-12362	NN	denotes	wk
T8187	12363-12369	NN	denotes	alphaA
T8189	12369-12370	HYPH	denotes	/
T8188	12370-12373	NN	denotes	BKO
T8190	12374-12379	NN	denotes	mouse
T8186	12380-12384	NN	denotes	lens
T8191	12385-12392	VBG	denotes	showing
T8192	12393-12398	JJ	denotes	dense
T8194	12399-12404	JJ	denotes	whole
T8195	12405-12409	NN	denotes	lens
T8193	12410-12418	NN	denotes	cataract
T8196	12418-12419	.	denotes	.
T8197	12419-12509	sentence	denotes	(D-F) Representative clear lenses from 5 wk (D), 46 wk (E), and wk 72 (F) wild type mice.
T8198	12420-12421	-LRB-	denotes	(
T8199	12421-12422	LS	denotes	D
T8201	12422-12423	SYM	denotes	-
T8202	12423-12424	LS	denotes	F
T8203	12424-12425	-RRB-	denotes	)
T8204	12426-12440	JJ	denotes	Representative
T8205	12441-12446	JJ	denotes	clear
T8200	12447-12453	NNS	denotes	lenses
T8206	12454-12458	IN	denotes	from
T8207	12459-12460	CD	denotes	5
T8208	12461-12463	NN	denotes	wk
T8210	12464-12465	-LRB-	denotes	(
T8211	12465-12466	NN	denotes	D
T8212	12466-12467	-RRB-	denotes	)
T8213	12467-12469	,	denotes	,
T8214	12469-12471	CD	denotes	46
T8215	12472-12474	NN	denotes	wk
T8216	12475-12476	-LRB-	denotes	(
T8217	12476-12477	NN	denotes	E
T8218	12477-12478	-RRB-	denotes	)
T8219	12478-12480	,	denotes	,
T8220	12480-12483	CC	denotes	and
T8221	12484-12486	NN	denotes	wk
T8222	12487-12489	CD	denotes	72
T8223	12490-12491	-LRB-	denotes	(
T8224	12491-12492	NN	denotes	F
T8225	12492-12493	-RRB-	denotes	)
T8226	12494-12498	JJ	denotes	wild
T8227	12499-12503	NN	denotes	type
T8209	12504-12508	NNS	denotes	mice
T8228	12508-12509	.	denotes	.
T8229	12509-12605	sentence	denotes	Arrows (A) indicate vacuoles in the equatorial region of a representative 5 wk alphaA/BKO lens.
T8230	12510-12516	NNS	denotes	Arrows
T8232	12517-12518	-LRB-	denotes	(
T8233	12518-12519	NN	denotes	A
T8234	12519-12520	-RRB-	denotes	)
T8231	12521-12529	VBP	denotes	indicate
T8235	12530-12538	NNS	denotes	vacuoles
T8236	12539-12541	IN	denotes	in
T8237	12542-12545	DT	denotes	the
T8239	12546-12556	JJ	denotes	equatorial
T8238	12557-12563	NN	denotes	region
T8240	12564-12566	IN	denotes	of
T8241	12567-12568	DT	denotes	a
T8243	12569-12583	JJ	denotes	representative
T8244	12584-12585	CD	denotes	5
T8245	12586-12588	NN	denotes	wk
T8246	12589-12595	NN	denotes	alphaA
T8248	12595-12596	HYPH	denotes	/
T8247	12596-12599	NN	denotes	BKO
T8242	12600-12604	NN	denotes	lens
T8249	12604-12605	.	denotes	.
T8250	12605-12739	sentence	denotes	The arrowheads (B) indicate an area of minor light scattering deep to the posterior capsule in a representative 5 wk alphaA/BKO lens.
T8251	12606-12609	DT	denotes	The
T8252	12610-12620	NNS	denotes	arrowheads
T8254	12621-12622	-LRB-	denotes	(
T8255	12622-12623	NN	denotes	B
T8256	12623-12624	-RRB-	denotes	)
T8253	12625-12633	VBP	denotes	indicate
T8257	12634-12636	DT	denotes	an
T8258	12637-12641	NN	denotes	area
T8259	12642-12644	IN	denotes	of
T8260	12645-12650	JJ	denotes	minor
T8261	12651-12656	NN	denotes	light
T8262	12657-12667	VBG	denotes	scattering
T8263	12668-12672	RB	denotes	deep
T8264	12673-12675	IN	denotes	to
T8265	12676-12679	DT	denotes	the
T8267	12680-12689	JJ	denotes	posterior
T8266	12690-12697	NN	denotes	capsule
T8268	12698-12700	IN	denotes	in
T8269	12701-12702	DT	denotes	a
T8271	12703-12717	JJ	denotes	representative
T8272	12718-12719	CD	denotes	5
T8273	12720-12722	NN	denotes	wk
T8274	12723-12729	NN	denotes	alphaA
T8276	12729-12730	HYPH	denotes	/
T8275	12730-12733	NN	denotes	BKO
T8270	12734-12738	NN	denotes	lens
T8277	12738-12739	.	denotes	.
T8890	12749-12759	NNS	denotes	Dimensions
T8891	12760-12763	CC	denotes	and
T8892	12764-12769	JJ	denotes	gross
T8894	12770-12780	JJ	denotes	lenticular
T8893	12781-12791	NN	denotes	appearance
T8895	12792-12794	IN	denotes	of
T8896	12795-12801	NNS	denotes	lenses
T8897	12801-12802	.	denotes	.
T3525	13287-13288	NN	denotes	E
T3524	13287-13288	sentence	denotes	E
T3527	13288-13298	NN	denotes	xamination
T3526	13288-13486	sentence	denotes	xamination of mid-axial sections stained with DiI and SYTOX green revealed unique differences in gross morphology and nucleic acid staining between alphaA/BKO lenses and wild type lenses (Figure 2).
T3529	13299-13301	IN	denotes	of
T3530	13302-13311	JJ	denotes	mid-axial
T3531	13312-13320	NNS	denotes	sections
T3532	13321-13328	VBN	denotes	stained
T3533	13329-13333	IN	denotes	with
T3534	13334-13337	NN	denotes	DiI
T3535	13338-13341	CC	denotes	and
T3536	13342-13347	NN	denotes	SYTOX
T3537	13348-13353	NN	denotes	green
T3528	13354-13362	VBD	denotes	revealed
T3538	13363-13369	JJ	denotes	unique
T3539	13370-13381	NNS	denotes	differences
T3540	13382-13384	IN	denotes	in
T3541	13385-13390	JJ	denotes	gross
T3542	13391-13401	NN	denotes	morphology
T3543	13402-13405	CC	denotes	and
T3544	13406-13413	JJ	denotes	nucleic
T3545	13414-13418	NN	denotes	acid
T3546	13419-13427	NN	denotes	staining
T3547	13428-13435	IN	denotes	between
T3548	13436-13442	NN	denotes	alphaA
T3550	13442-13443	HYPH	denotes	/
T3549	13443-13446	NN	denotes	BKO
T3551	13447-13453	NNS	denotes	lenses
T3552	13454-13457	CC	denotes	and
T3553	13458-13462	JJ	denotes	wild
T3554	13463-13467	NN	denotes	type
T3555	13468-13474	NNS	denotes	lenses
T3556	13475-13476	-LRB-	denotes	(
T3557	13476-13482	NN	denotes	Figure
T3558	13483-13484	CD	denotes	2
T3559	13484-13485	-RRB-	denotes	)
T3560	13485-13486	.	denotes	.
T3561	13486-13648	sentence	denotes	In five week old alphaA/BKO lenses, Sytox green stained nuclei (green) in the anterior epithelium, equatorial/bow, and posterior subcapsular regions (Figure 2A).
T3562	13487-13489	IN	denotes	In
T3564	13490-13494	CD	denotes	five
T3565	13495-13499	NN	denotes	week
T3566	13500-13503	JJ	denotes	old
T3568	13504-13510	NN	denotes	alphaA
T3570	13510-13511	HYPH	denotes	/
T3569	13511-13514	NN	denotes	BKO
T3567	13515-13521	NNS	denotes	lenses
T3571	13521-13523	,	denotes	,
T3572	13523-13528	NN	denotes	Sytox
T3573	13529-13534	JJ	denotes	green
T3574	13535-13542	VBN	denotes	stained
T3563	13543-13549	NNS	denotes	nuclei
T3575	13550-13551	-LRB-	denotes	(
T3576	13551-13556	JJ	denotes	green
T3577	13556-13557	-RRB-	denotes	)
T3578	13558-13560	IN	denotes	in
T3579	13561-13564	DT	denotes	the
T3581	13565-13573	JJ	denotes	anterior
T3582	13574-13584	NN	denotes	epithelium
T3583	13584-13586	,	denotes	,
T3584	13586-13596	JJ	denotes	equatorial
T3586	13596-13597	HYPH	denotes	/
T3585	13597-13600	NN	denotes	bow
T3587	13600-13602	,	denotes	,
T3588	13602-13605	CC	denotes	and
T3589	13606-13615	JJ	denotes	posterior
T3590	13616-13627	JJ	denotes	subcapsular
T3580	13628-13635	NNS	denotes	regions
T3591	13636-13637	-LRB-	denotes	(
T3593	13637-13643	NN	denotes	Figure
T3592	13644-13646	NN	denotes	2A
T3594	13646-13647	-RRB-	denotes	)
T3595	13647-13648	.	denotes	.
T3596	13648-13743	sentence	denotes	Cell nuclei localized to the equatorial/bow region were disorganized as compared to wild type.
T3597	13649-13653	NN	denotes	Cell
T3598	13654-13660	NNS	denotes	nuclei
T3600	13661-13670	VBN	denotes	localized
T3601	13671-13673	IN	denotes	to
T3602	13674-13677	DT	denotes	the
T3604	13678-13688	JJ	denotes	equatorial
T3606	13688-13689	HYPH	denotes	/
T3605	13689-13692	JJ	denotes	bow
T3603	13693-13699	NN	denotes	region
T3607	13700-13704	VBD	denotes	were
T3599	13705-13717	VBN	denotes	disorganized
T3608	13718-13720	IN	denotes	as
T3609	13721-13729	VBN	denotes	compared
T3610	13730-13732	IN	denotes	to
T3611	13733-13737	JJ	denotes	wild
T3612	13738-13742	NN	denotes	type
T3613	13742-13743	.	denotes	.
T3614	13743-13858	sentence	denotes	There were enlarged extracellular spaces between cells in the equatorial/bow region in 5 wk old alphaA/BKO lenses.
T3615	13744-13749	EX	denotes	There
T3616	13750-13754	VBD	denotes	were
T3617	13755-13763	VBN	denotes	enlarged
T3619	13764-13777	JJ	denotes	extracellular
T3618	13778-13784	NNS	denotes	spaces
T3620	13785-13792	IN	denotes	between
T3621	13793-13798	NNS	denotes	cells
T3622	13799-13801	IN	denotes	in
T3623	13802-13805	DT	denotes	the
T3625	13806-13816	JJ	denotes	equatorial
T3627	13816-13817	HYPH	denotes	/
T3626	13817-13820	JJ	denotes	bow
T3624	13821-13827	NN	denotes	region
T3628	13828-13830	IN	denotes	in
T3629	13831-13832	CD	denotes	5
T3630	13833-13835	NN	denotes	wk
T3631	13836-13839	JJ	denotes	old
T3633	13840-13846	NN	denotes	alphaA
T3635	13846-13847	HYPH	denotes	/
T3634	13847-13850	NN	denotes	BKO
T3632	13851-13857	NNS	denotes	lenses
T3636	13857-13858	.	denotes	.
T3637	13858-14127	sentence	denotes	Fifty-four wk old alphaA/BKO lenses (Figure 2B) did not stain for nucleic acid in the posterior subcapsular region, however, the entire anterior subcapsular lens region stained for nucleic acid (refer to Figure 3I for high magnification/resolution image of this area).
T3638	13859-13864	CD	denotes	Fifty
T3640	13864-13865	HYPH	denotes	-
T3639	13865-13869	CD	denotes	four
T3641	13870-13872	NN	denotes	wk
T3642	13873-13876	JJ	denotes	old
T3644	13877-13883	NN	denotes	alphaA
T3646	13883-13884	HYPH	denotes	/
T3645	13884-13887	NN	denotes	BKO
T3643	13888-13894	NNS	denotes	lenses
T3648	13895-13896	-LRB-	denotes	(
T3650	13896-13902	NN	denotes	Figure
T3649	13903-13905	NN	denotes	2B
T3651	13905-13906	-RRB-	denotes	)
T3652	13907-13910	VBD	denotes	did
T3653	13911-13914	RB	denotes	not
T3647	13915-13920	VB	denotes	stain
T3655	13921-13924	IN	denotes	for
T3656	13925-13932	JJ	denotes	nucleic
T3657	13933-13937	NN	denotes	acid
T3658	13938-13940	IN	denotes	in
T3659	13941-13944	DT	denotes	the
T3661	13945-13954	JJ	denotes	posterior
T3662	13955-13966	JJ	denotes	subcapsular
T3660	13967-13973	NN	denotes	region
T3663	13973-13975	,	denotes	,
T3664	13975-13982	RB	denotes	however
T3665	13982-13984	,	denotes	,
T3666	13984-13987	DT	denotes	the
T3668	13988-13994	JJ	denotes	entire
T3669	13995-14003	JJ	denotes	anterior
T3670	14004-14015	JJ	denotes	subcapsular
T3671	14016-14020	NN	denotes	lens
T3667	14021-14027	NN	denotes	region
T3654	14028-14035	VBN	denotes	stained
T3672	14036-14039	IN	denotes	for
T3673	14040-14047	JJ	denotes	nucleic
T3674	14048-14052	NN	denotes	acid
T3675	14053-14054	-LRB-	denotes	(
T3676	14054-14059	VB	denotes	refer
T3677	14060-14062	IN	denotes	to
T3678	14063-14069	NN	denotes	Figure
T3679	14070-14072	NN	denotes	3I
T3680	14073-14076	IN	denotes	for
T3681	14077-14081	JJ	denotes	high
T3683	14082-14095	NN	denotes	magnification
T3685	14095-14096	HYPH	denotes	/
T3684	14096-14106	NN	denotes	resolution
T3682	14107-14112	NN	denotes	image
T3686	14113-14115	IN	denotes	of
T3687	14116-14120	DT	denotes	this
T3688	14121-14125	NN	denotes	area
T3689	14125-14126	-RRB-	denotes	)
T3690	14126-14127	.	denotes	.
T3691	14127-14278	sentence	denotes	A defined equatorial/bow region was not observed in these older lenses, and fiber cells extending to the posterior capsule of the lens were not found.
T3692	14128-14129	DT	denotes	A
T3694	14130-14137	VBN	denotes	defined
T3695	14138-14148	JJ	denotes	equatorial
T3697	14148-14149	HYPH	denotes	/
T3696	14149-14152	JJ	denotes	bow
T3693	14153-14159	NN	denotes	region
T3699	14160-14163	VBD	denotes	was
T3700	14164-14167	RB	denotes	not
T3698	14168-14176	VBN	denotes	observed
T3701	14177-14179	IN	denotes	in
T3702	14180-14185	DT	denotes	these
T3704	14186-14191	JJR	denotes	older
T3703	14192-14198	NNS	denotes	lenses
T3705	14198-14200	,	denotes	,
T3706	14200-14203	CC	denotes	and
T3707	14204-14209	NN	denotes	fiber
T3708	14210-14215	NNS	denotes	cells
T3710	14216-14225	VBG	denotes	extending
T3711	14226-14228	IN	denotes	to
T3712	14229-14232	DT	denotes	the
T3714	14233-14242	JJ	denotes	posterior
T3713	14243-14250	NN	denotes	capsule
T3715	14251-14253	IN	denotes	of
T3716	14254-14257	DT	denotes	the
T3717	14258-14262	NN	denotes	lens
T3718	14263-14267	VBD	denotes	were
T3719	14268-14271	RB	denotes	not
T3709	14272-14277	VBN	denotes	found
T3720	14277-14278	.	denotes	.
T3721	14278-14500	sentence	denotes	Disorganized cellular material capped the anterior region of the embryonic/fetal nucleus, and a distinctive indentation of the lens mass at the equatorial region was apparent in mid-axial sections as well as whole lenses.
T3722	14279-14291	JJ	denotes	Disorganized
T3724	14292-14300	JJ	denotes	cellular
T3723	14301-14309	NN	denotes	material
T3725	14310-14316	VBD	denotes	capped
T3726	14317-14320	DT	denotes	the
T3728	14321-14329	JJ	denotes	anterior
T3727	14330-14336	NN	denotes	region
T3729	14337-14339	IN	denotes	of
T3730	14340-14343	DT	denotes	the
T3732	14344-14353	JJ	denotes	embryonic
T3734	14353-14354	HYPH	denotes	/
T3733	14354-14359	JJ	denotes	fetal
T3731	14360-14367	NN	denotes	nucleus
T3735	14367-14369	,	denotes	,
T3736	14369-14372	CC	denotes	and
T3737	14373-14374	DT	denotes	a
T3739	14375-14386	JJ	denotes	distinctive
T3738	14387-14398	NN	denotes	indentation
T3741	14399-14401	IN	denotes	of
T3742	14402-14405	DT	denotes	the
T3744	14406-14410	NN	denotes	lens
T3743	14411-14415	NN	denotes	mass
T3745	14416-14418	IN	denotes	at
T3746	14419-14422	DT	denotes	the
T3748	14423-14433	JJ	denotes	equatorial
T3747	14434-14440	NN	denotes	region
T3740	14441-14444	VBD	denotes	was
T3749	14445-14453	JJ	denotes	apparent
T3750	14454-14456	IN	denotes	in
T3751	14457-14466	JJ	denotes	mid-axial
T3752	14467-14475	NNS	denotes	sections
T3753	14476-14478	RB	denotes	as
T3755	14479-14483	RB	denotes	well
T3754	14484-14486	IN	denotes	as
T3756	14487-14492	JJ	denotes	whole
T3757	14493-14499	NNS	denotes	lenses
T3758	14499-14500	.	denotes	.
T3759	14500-14654	sentence	denotes	In alphaA/BKO lenses the embryonic/fetal nucleus (primary lens fibers) had migrated posteriorly and appeared to be in contact with the posterior capsule.
T3760	14501-14503	IN	denotes	In
T3762	14504-14510	NN	denotes	alphaA
T3764	14510-14511	HYPH	denotes	/
T3763	14511-14514	NN	denotes	BKO
T3765	14515-14521	NNS	denotes	lenses
T3766	14522-14525	DT	denotes	the
T3768	14526-14535	JJ	denotes	embryonic
T3770	14535-14536	HYPH	denotes	/
T3769	14536-14541	JJ	denotes	fetal
T3767	14542-14549	NN	denotes	nucleus
T3771	14550-14551	-LRB-	denotes	(
T3772	14551-14558	JJ	denotes	primary
T3774	14559-14563	NN	denotes	lens
T3773	14564-14570	NNS	denotes	fibers
T3775	14570-14571	-RRB-	denotes	)
T3776	14572-14575	VBD	denotes	had
T3761	14576-14584	VBN	denotes	migrated
T3777	14585-14596	RB	denotes	posteriorly
T3778	14597-14600	CC	denotes	and
T3779	14601-14609	VBD	denotes	appeared
T3780	14610-14612	TO	denotes	to
T3781	14613-14615	VB	denotes	be
T3782	14616-14618	IN	denotes	in
T3783	14619-14626	NN	denotes	contact
T3784	14627-14631	IN	denotes	with
T3785	14632-14635	DT	denotes	the
T3787	14636-14645	JJ	denotes	posterior
T3786	14646-14653	NN	denotes	capsule
T3788	14653-14654	.	denotes	.
T3789	14654-14898	sentence	denotes	In 5 wk, 46 wk or 72 wk old wild type lenses (Figure 2C,2D and 2E), however, there was no posterior or anterior subcapsular nucleic acid staining and the embryonic-fetal nucleus was centrally located, not in contact with the posterior capsule.
T3790	14655-14657	IN	denotes	In
T3792	14658-14659	CD	denotes	5
T3793	14660-14662	NN	denotes	wk
T3795	14662-14664	,	denotes	,
T3796	14664-14666	CD	denotes	46
T3797	14667-14669	NN	denotes	wk
T3798	14670-14672	CC	denotes	or
T3799	14673-14675	CD	denotes	72
T3800	14676-14678	NN	denotes	wk
T3794	14679-14682	JJ	denotes	old
T3802	14683-14687	JJ	denotes	wild
T3803	14688-14692	NN	denotes	type
T3801	14693-14699	NNS	denotes	lenses
T3804	14700-14701	-LRB-	denotes	(
T3806	14701-14707	NN	denotes	Figure
T3805	14708-14710	NN	denotes	2C
T3807	14710-14711	,	denotes	,
T3808	14711-14713	NN	denotes	2D
T3809	14714-14717	CC	denotes	and
T3810	14718-14720	NN	denotes	2E
T3811	14720-14721	-RRB-	denotes	)
T3812	14721-14723	,	denotes	,
T3813	14723-14730	RB	denotes	however
T3814	14730-14732	,	denotes	,
T3815	14732-14737	EX	denotes	there
T3791	14738-14741	VBD	denotes	was
T3816	14742-14744	DT	denotes	no
T3818	14745-14754	JJ	denotes	posterior
T3819	14755-14757	CC	denotes	or
T3820	14758-14766	JJ	denotes	anterior
T3821	14767-14778	JJ	denotes	subcapsular
T3822	14779-14786	JJ	denotes	nucleic
T3823	14787-14791	NN	denotes	acid
T3817	14792-14800	NN	denotes	staining
T3824	14801-14804	CC	denotes	and
T3825	14805-14808	DT	denotes	the
T3827	14809-14818	JJ	denotes	embryonic
T3829	14818-14819	HYPH	denotes	-
T3828	14819-14824	JJ	denotes	fetal
T3826	14825-14832	NN	denotes	nucleus
T3830	14833-14836	VBD	denotes	was
T3831	14837-14846	RB	denotes	centrally
T3832	14847-14854	JJ	denotes	located
T3833	14854-14856	,	denotes	,
T3834	14856-14859	CC	denotes	not
T3835	14860-14862	IN	denotes	in
T3836	14863-14870	NN	denotes	contact
T3837	14871-14875	IN	denotes	with
T3838	14876-14879	DT	denotes	the
T3840	14880-14889	JJ	denotes	posterior
T3839	14890-14897	NN	denotes	capsule
T3841	14897-14898	.	denotes	.
T3842	14898-14899	sentence	denotes
T8300	14909-14923	JJ	denotes	Representative
T8302	14924-14932	JJ	denotes	confocal
T8303	14933-14940	JJ	denotes	optical
T8301	14941-14949	NNS	denotes	sections
T8304	14950-14955	VBN	denotes	taken
T8305	14956-14960	IN	denotes	from
T8306	14961-14970	JJ	denotes	mid-axial
T8308	14971-14980	NN	denotes	vibratome
T8307	14981-14989	NNS	denotes	sections
T8309	14990-14992	IN	denotes	of
T8310	14993-14999	NN	denotes	alphaA
T8312	14999-15000	HYPH	denotes	/
T8311	15000-15003	NN	denotes	BKO
T8313	15004-15005	-LRB-	denotes	(
T8315	15005-15006	NN	denotes	A
T8316	15006-15007	SYM	denotes	&
T8314	15007-15008	NN	denotes	B
T8317	15008-15009	-RRB-	denotes	)
T8318	15010-15013	CC	denotes	and
T8319	15014-15018	JJ	denotes	wild
T8320	15019-15023	NN	denotes	type
T8322	15024-15025	-LRB-	denotes	(
T8323	15025-15026	NN	denotes	C
T8324	15026-15027	SYM	denotes	-
T8325	15027-15028	NN	denotes	E
T8326	15028-15029	-RRB-	denotes	)
T8321	15030-15036	NNS	denotes	lenses
T8327	15036-15037	.	denotes	.
T8328	15037-15121	sentence	denotes	All optical sections were generated using a 4 × /0.10 objective on a Zeiss LSM 410.
T8329	15038-15041	DT	denotes	All
T8331	15042-15049	JJ	denotes	optical
T8330	15050-15058	NNS	denotes	sections
T8333	15059-15063	VBD	denotes	were
T8332	15064-15073	VBN	denotes	generated
T8334	15074-15079	VBG	denotes	using
T8335	15080-15081	DT	denotes	a
T8337	15082-15083	CD	denotes	4
T8338	15084-15085	SYM	denotes	×
T8340	15086-15087	SYM	denotes	/
T8339	15087-15091	CD	denotes	0.10
T8336	15092-15101	NN	denotes	objective
T8341	15102-15104	IN	denotes	on
T8342	15105-15106	DT	denotes	a
T8344	15107-15112	NNP	denotes	Zeiss
T8343	15113-15116	NNP	denotes	LSM
T8345	15117-15120	CD	denotes	410
T8346	15120-15121	.	denotes	.
T8347	15121-15232	sentence	denotes	The red color represents DiI staining (membrane), while the green color represents SYTOX green staining (DNA).
T8348	15122-15125	DT	denotes	The
T8350	15126-15129	JJ	denotes	red
T8349	15130-15135	NN	denotes	color
T8351	15136-15146	VBZ	denotes	represents
T8352	15147-15150	NN	denotes	DiI
T8353	15151-15159	NN	denotes	staining
T8354	15160-15161	-LRB-	denotes	(
T8355	15161-15169	NN	denotes	membrane
T8356	15169-15170	-RRB-	denotes	)
T8357	15170-15172	,	denotes	,
T8358	15172-15177	IN	denotes	while
T8360	15178-15181	DT	denotes	the
T8362	15182-15187	JJ	denotes	green
T8361	15188-15193	NN	denotes	color
T8359	15194-15204	VBZ	denotes	represents
T8363	15205-15210	NN	denotes	SYTOX
T8364	15211-15216	JJ	denotes	green
T8365	15217-15225	NN	denotes	staining
T8366	15226-15227	-LRB-	denotes	(
T8367	15227-15230	NN	denotes	DNA
T8368	15230-15231	-RRB-	denotes	)
T8369	15231-15232	.	denotes	.
T8370	15232-15302	sentence	denotes	(A&B) were taken from 5 wk and 54 wk alphaA/BKO lenses, respectively.
T8371	15233-15234	-LRB-	denotes	(
T8373	15234-15235	NN	denotes	A
T8374	15235-15236	CC	denotes	&
T8375	15236-15237	NN	denotes	B
T8376	15237-15238	-RRB-	denotes	)
T8377	15239-15243	VBD	denotes	were
T8372	15244-15249	VBN	denotes	taken
T8378	15250-15254	IN	denotes	from
T8379	15255-15256	CD	denotes	5
T8380	15257-15259	NN	denotes	wk
T8382	15260-15263	CC	denotes	and
T8383	15264-15266	CD	denotes	54
T8384	15267-15269	NN	denotes	wk
T8385	15270-15276	NN	denotes	alphaA
T8387	15276-15277	HYPH	denotes	/
T8386	15277-15280	NN	denotes	BKO
T8381	15281-15287	NNS	denotes	lenses
T8388	15287-15289	,	denotes	,
T8389	15289-15301	RB	denotes	respectively
T8390	15301-15302	.	denotes	.
T8391	15302-15379	sentence	denotes	(C-E) were taken from 5 wk, 46 wk, and 72 wk wild type lenses, respectively.
T8392	15303-15304	-LRB-	denotes	(
T8394	15304-15305	NN	denotes	C
T8395	15305-15306	SYM	denotes	-
T8396	15306-15307	NN	denotes	E
T8397	15307-15308	-RRB-	denotes	)
T8398	15309-15313	VBD	denotes	were
T8393	15314-15319	VBN	denotes	taken
T8399	15320-15324	IN	denotes	from
T8400	15325-15326	CD	denotes	5
T8401	15327-15329	NN	denotes	wk
T8403	15329-15331	,	denotes	,
T8404	15331-15333	CD	denotes	46
T8405	15334-15336	NN	denotes	wk
T8406	15336-15338	,	denotes	,
T8407	15338-15341	CC	denotes	and
T8408	15342-15344	CD	denotes	72
T8409	15345-15347	NN	denotes	wk
T8410	15348-15352	JJ	denotes	wild
T8411	15353-15357	NN	denotes	type
T8402	15358-15364	NNS	denotes	lenses
T8412	15364-15366	,	denotes	,
T8413	15366-15378	RB	denotes	respectively
T8414	15378-15379	.	denotes	.
T8415	15379-15471	sentence	denotes	The arrowheads indicate representative anterior epithelial nuclei stained with SYTOX green.
T8416	15380-15383	DT	denotes	The
T8417	15384-15394	NNS	denotes	arrowheads
T8418	15395-15403	VBP	denotes	indicate
T8419	15404-15418	JJ	denotes	representative
T8421	15419-15427	JJ	denotes	anterior
T8422	15428-15438	JJ	denotes	epithelial
T8420	15439-15445	NNS	denotes	nuclei
T8423	15446-15453	VBN	denotes	stained
T8424	15454-15458	IN	denotes	with
T8425	15459-15464	NN	denotes	SYTOX
T8426	15465-15470	NN	denotes	green
T8427	15470-15471	.	denotes	.
T8471	15482-15496	JJ	denotes	Representative
T8473	15497-15505	JJ	denotes	confocal
T8474	15506-15513	JJ	denotes	optical
T8472	15514-15522	NNS	denotes	sections
T8475	15523-15527	IN	denotes	from
T8476	15528-15534	NN	denotes	alphaA
T8478	15534-15535	HYPH	denotes	/
T8477	15535-15538	NN	denotes	BKO
T8480	15539-15540	-LRB-	denotes	(
T8481	15540-15541	NN	denotes	A
T8482	15541-15542	SYM	denotes	-
T8483	15542-15543	NN	denotes	J
T8484	15543-15544	-RRB-	denotes	)
T8485	15545-15548	CC	denotes	and
T8486	15549-15553	JJ	denotes	wild
T8487	15554-15558	NN	denotes	type
T8488	15559-15560	-LRB-	denotes	(
T8489	15560-15561	NN	denotes	K
T8490	15561-15562	SYM	denotes	-
T8491	15562-15563	NN	denotes	T
T8492	15563-15564	-RRB-	denotes	)
T8479	15565-15571	NNS	denotes	lenses
T8493	15571-15572	.	denotes	.
T8494	15572-15656	sentence	denotes	All optical sections were generated using a 63 × /1.4 objective on a Zeiss LSM 410.
T8495	15573-15576	DT	denotes	All
T8497	15577-15584	JJ	denotes	optical
T8496	15585-15593	NNS	denotes	sections
T8499	15594-15598	VBD	denotes	were
T8498	15599-15608	VBN	denotes	generated
T8500	15609-15614	VBG	denotes	using
T8501	15615-15616	DT	denotes	a
T8503	15617-15619	CD	denotes	63
T8504	15620-15621	SYM	denotes	×
T8506	15622-15623	SYM	denotes	/
T8505	15623-15626	CD	denotes	1.4
T8502	15627-15636	NN	denotes	objective
T8507	15637-15639	IN	denotes	on
T8508	15640-15641	DT	denotes	a
T8510	15642-15647	NNP	denotes	Zeiss
T8509	15648-15651	NNP	denotes	LSM
T8511	15652-15655	CD	denotes	410
T8512	15655-15656	.	denotes	.
T8513	15656-15767	sentence	denotes	The red color represents DiI staining (membrane), while the green color represents SYTOX green staining (DNA).
T8514	15657-15660	DT	denotes	The
T8516	15661-15664	JJ	denotes	red
T8515	15665-15670	NN	denotes	color
T8517	15671-15681	VBZ	denotes	represents
T8518	15682-15685	NN	denotes	DiI
T8519	15686-15694	NN	denotes	staining
T8520	15695-15696	-LRB-	denotes	(
T8521	15696-15704	NN	denotes	membrane
T8522	15704-15705	-RRB-	denotes	)
T8523	15705-15707	,	denotes	,
T8524	15707-15712	IN	denotes	while
T8526	15713-15716	DT	denotes	the
T8528	15717-15722	JJ	denotes	green
T8527	15723-15728	NN	denotes	color
T8525	15729-15739	VBZ	denotes	represents
T8529	15740-15745	NN	denotes	SYTOX
T8530	15746-15751	JJ	denotes	green
T8531	15752-15760	NN	denotes	staining
T8532	15761-15762	-LRB-	denotes	(
T8533	15762-15765	NN	denotes	DNA
T8534	15765-15766	-RRB-	denotes	)
T8535	15766-15767	.	denotes	.
T8536	15767-15806	sentence	denotes	(A-E) are from 5 wk alphaA/BKO lenses.
T8537	15768-15769	-LRB-	denotes	(
T8539	15769-15770	NN	denotes	A
T8540	15770-15771	SYM	denotes	-
T8541	15771-15772	NN	denotes	E
T8542	15772-15773	-RRB-	denotes	)
T8538	15774-15777	VBP	denotes	are
T8543	15778-15782	IN	denotes	from
T8544	15783-15784	CD	denotes	5
T8545	15785-15787	NN	denotes	wk
T8547	15788-15794	NN	denotes	alphaA
T8549	15794-15795	HYPH	denotes	/
T8548	15795-15798	NN	denotes	BKO
T8546	15799-15805	NNS	denotes	lenses
T8550	15805-15806	.	denotes	.
T8551	15806-15846	sentence	denotes	(F-J) are from 54 wk alphaA/BKO lenses.
T8552	15807-15808	-LRB-	denotes	(
T8554	15808-15809	NN	denotes	F
T8555	15809-15810	SYM	denotes	-
T8556	15810-15811	NN	denotes	J
T8557	15811-15812	-RRB-	denotes	)
T8553	15813-15816	VBP	denotes	are
T8558	15817-15821	IN	denotes	from
T8559	15822-15824	CD	denotes	54
T8560	15825-15827	NN	denotes	wk
T8562	15828-15834	NN	denotes	alphaA
T8564	15834-15835	HYPH	denotes	/
T8563	15835-15838	NN	denotes	BKO
T8561	15839-15845	NNS	denotes	lenses
T8565	15845-15846	.	denotes	.
T8566	15846-15884	sentence	denotes	(K-O) are from 5 wk wild type lenses.
T8567	15847-15848	-LRB-	denotes	(
T8569	15848-15849	NN	denotes	K
T8570	15849-15850	SYM	denotes	-
T8571	15850-15851	NN	denotes	O
T8572	15851-15852	-RRB-	denotes	)
T8568	15853-15856	VBP	denotes	are
T8573	15857-15861	IN	denotes	from
T8574	15862-15863	CD	denotes	5
T8575	15864-15866	NN	denotes	wk
T8577	15867-15871	JJ	denotes	wild
T8578	15872-15876	NN	denotes	type
T8576	15877-15883	NNS	denotes	lenses
T8579	15883-15884	.	denotes	.
T8580	15884-15923	sentence	denotes	(P-T) are from 46 wk wild type lenses.
T8581	15885-15886	-LRB-	denotes	(
T8583	15886-15887	NN	denotes	P
T8584	15887-15888	SYM	denotes	-
T8585	15888-15889	NN	denotes	T
T8586	15889-15890	-RRB-	denotes	)
T8582	15891-15894	VBP	denotes	are
T8587	15895-15899	IN	denotes	from
T8588	15900-15902	CD	denotes	46
T8589	15903-15905	NN	denotes	wk
T8591	15906-15910	JJ	denotes	wild
T8592	15911-15915	NN	denotes	type
T8590	15916-15922	NNS	denotes	lenses
T8593	15922-15923	.	denotes	.
T8594	15923-15984	sentence	denotes	(A, F, K, and P) are anterior central cortex and epithelium.
T8595	15924-15925	-LRB-	denotes	(
T8597	15925-15926	NN	denotes	A
T8598	15926-15928	,	denotes	,
T8599	15928-15929	NN	denotes	F
T8600	15929-15931	,	denotes	,
T8601	15931-15932	NN	denotes	K
T8602	15932-15934	,	denotes	,
T8603	15934-15937	CC	denotes	and
T8604	15938-15939	NN	denotes	P
T8605	15939-15940	-RRB-	denotes	)
T8596	15941-15944	VBP	denotes	are
T8606	15945-15953	JJ	denotes	anterior
T8608	15954-15961	JJ	denotes	central
T8607	15962-15968	NN	denotes	cortex
T8609	15969-15972	CC	denotes	and
T8610	15973-15983	NN	denotes	epithelium
T8611	15983-15984	.	denotes	.
T8612	15984-16028	sentence	denotes	(B, G, L, and Q) are equatorial bow region.
T8613	15985-15986	-LRB-	denotes	(
T8615	15986-15987	NN	denotes	B
T8616	15987-15989	,	denotes	,
T8617	15989-15990	NN	denotes	G
T8618	15990-15992	,	denotes	,
T8619	15992-15993	NN	denotes	L
T8620	15993-15995	,	denotes	,
T8621	15995-15998	CC	denotes	and
T8622	15999-16000	NN	denotes	Q
T8623	16000-16001	-RRB-	denotes	)
T8614	16002-16005	VBP	denotes	are
T8624	16006-16016	JJ	denotes	equatorial
T8625	16017-16020	NN	denotes	bow
T8626	16021-16027	NN	denotes	region
T8627	16027-16028	.	denotes	.
T8628	16028-16074	sentence	denotes	(C, H, M, and R) are embryonic/fetal nucleus.
T8629	16029-16030	-LRB-	denotes	(
T8631	16030-16031	NN	denotes	C
T8632	16031-16033	,	denotes	,
T8633	16033-16034	NN	denotes	H
T8634	16034-16036	,	denotes	,
T8635	16036-16037	NN	denotes	M
T8636	16037-16039	,	denotes	,
T8637	16039-16042	CC	denotes	and
T8638	16043-16044	NN	denotes	R
T8639	16044-16045	-RRB-	denotes	)
T8630	16046-16049	VBP	denotes	are
T8640	16050-16059	JJ	denotes	embryonic
T8642	16059-16060	HYPH	denotes	/
T8641	16060-16065	JJ	denotes	fetal
T8643	16066-16073	NN	denotes	nucleus
T8644	16073-16074	.	denotes	.
T8645	16074-16117	sentence	denotes	(D, I, N, and S) are deep anterior cortex.
T8646	16075-16076	-LRB-	denotes	(
T8648	16076-16077	NN	denotes	D
T8649	16077-16079	,	denotes	,
T8650	16079-16080	NN	denotes	I
T8651	16080-16082	,	denotes	,
T8652	16082-16083	NN	denotes	N
T8653	16083-16085	,	denotes	,
T8654	16085-16088	CC	denotes	and
T8655	16089-16090	NN	denotes	S
T8656	16090-16091	-RRB-	denotes	)
T8647	16092-16095	VBP	denotes	are
T8657	16096-16100	JJ	denotes	deep
T8659	16101-16109	JJ	denotes	anterior
T8658	16110-16116	NN	denotes	cortex
T8660	16116-16117	.	denotes	.
T8661	16117-16168	sentence	denotes	(E, J, O, and T) are posterior subcapsular region.
T8662	16118-16119	-LRB-	denotes	(
T8664	16119-16120	NN	denotes	E
T8665	16120-16122	,	denotes	,
T8666	16122-16123	NN	denotes	J
T8667	16123-16125	,	denotes	,
T8668	16125-16126	NN	denotes	O
T8669	16126-16128	,	denotes	,
T8670	16128-16131	CC	denotes	and
T8671	16132-16133	NN	denotes	T
T8672	16133-16134	-RRB-	denotes	)
T8663	16135-16138	VBP	denotes	are
T8673	16139-16148	JJ	denotes	posterior
T8675	16149-16160	JJ	denotes	subcapsular
T8674	16161-16167	NN	denotes	region
T8676	16167-16168	.	denotes	.
T8677	16168-16227	sentence	denotes	(*) indicates areas devoid of cellular material (B and G).
T8678	16169-16170	-LRB-	denotes	(
T8680	16170-16171	SYM	denotes	*
T8681	16171-16172	-RRB-	denotes	)
T8679	16173-16182	VBZ	denotes	indicates
T8682	16183-16188	NNS	denotes	areas
T8683	16189-16195	JJ	denotes	devoid
T8684	16196-16198	IN	denotes	of
T8685	16199-16207	JJ	denotes	cellular
T8686	16208-16216	NN	denotes	material
T8687	16217-16218	-LRB-	denotes	(
T8688	16218-16219	NN	denotes	B
T8689	16220-16223	CC	denotes	and
T8690	16224-16225	NN	denotes	G
T8691	16225-16226	-RRB-	denotes	)
T8692	16226-16227	.	denotes	.
T8693	16227-16265	sentence	denotes	Arrow indicates posterior suture (O).
T8694	16228-16233	NNP	denotes	Arrow
T8695	16234-16243	VBZ	denotes	indicates
T8696	16244-16253	JJ	denotes	posterior
T8697	16254-16260	NN	denotes	suture
T8698	16261-16262	-LRB-	denotes	(
T8699	16262-16263	NN	denotes	O
T8700	16263-16264	-RRB-	denotes	)
T8701	16264-16265	.	denotes	.
T8702	16265-16368	sentence	denotes	Arrowheads indicate representative nuclei stained with SYTOX green (A, B, E, F, G, I, K, L, P, and Q).
T8703	16266-16276	NNS	denotes	Arrowheads
T8704	16277-16285	VBP	denotes	indicate
T8705	16286-16300	JJ	denotes	representative
T8706	16301-16307	NNS	denotes	nuclei
T8707	16308-16315	VBN	denotes	stained
T8708	16316-16320	IN	denotes	with
T8709	16321-16326	NN	denotes	SYTOX
T8710	16327-16332	NN	denotes	green
T8711	16333-16334	-LRB-	denotes	(
T8712	16334-16335	NN	denotes	A
T8713	16335-16337	,	denotes	,
T8714	16337-16338	NN	denotes	B
T8715	16338-16340	,	denotes	,
T8716	16340-16341	NN	denotes	E
T8717	16341-16343	,	denotes	,
T8718	16343-16344	NN	denotes	F
T8719	16344-16346	,	denotes	,
T8720	16346-16347	NN	denotes	G
T8721	16347-16349	,	denotes	,
T8722	16349-16350	NN	denotes	I
T8723	16350-16352	,	denotes	,
T8724	16352-16353	NN	denotes	K
T8725	16353-16355	,	denotes	,
T8726	16355-16356	NN	denotes	L
T8727	16356-16358	,	denotes	,
T8728	16358-16359	NN	denotes	P
T8729	16359-16361	,	denotes	,
T8730	16361-16364	CC	denotes	and
T8731	16365-16366	NN	denotes	Q
T8732	16366-16367	-RRB-	denotes	)
T8733	16367-16368	.	denotes	.
T3844	16369-16370	NN	denotes	A
T3843	16369-16372	sentence	denotes	At
T3845	16370-16371	NN	denotes	t
T3847	16372-16373	DT	denotes	h
T3846	16372-16379	sentence	denotes	higher
T3848	16373-16374	NN	denotes	i
T3849	16374-16375	NN	denotes	g
T3850	16375-16377	IN	denotes	he
T3851	16377-16378	JJ	denotes	r
T3853	16379-16380	IN	denotes	m
T3852	16379-16381	sentence	denotes	ma
T3854	16380-16381	CC	denotes	a
T3856	16381-16383	NN	denotes	gn
T3855	16381-16383	sentence	denotes	gn
T3858	16383-16384	NN	denotes	i
T3857	16383-16384	sentence	denotes	i
T3861	16384-16385	JJ	denotes	f
T3859	16384-16392	sentence	denotes	fication
T3862	16385-16388	JJ	denotes	ica
T3860	16388-16392	NNS	denotes	tion
T3864	16392-16394	,	denotes	,
T3863	16392-16395	sentence	denotes	, e
T3865	16394-16395	IN	denotes	e
T3867	16395-16396	NN	denotes	x
T3866	16395-16398	sentence	denotes	xam
T3868	16396-16397	CC	denotes	a
T3869	16397-16398	NN	denotes	m
T3871	16398-16399	JJ	denotes	i
T3870	16398-16400	sentence	denotes	in
T3872	16399-16400	NN	denotes	n
T3874	16400-16401	CC	denotes	a
T3873	16400-16402	sentence	denotes	at
T3875	16401-16402	JJ	denotes	t
T3877	16402-16404	JJ	denotes	io
T3876	16402-16404	sentence	denotes	io
T3879	16404-16405	CC	denotes	n
T3878	16404-16407	sentence	denotes	n o
T3880	16406-16407	JJ	denotes	o
T3882	16407-16408	IN	denotes	f
T3881	16407-16409	sentence	denotes	f
T3884	16409-16410	VBZ	denotes	s
T3883	16409-16411	sentence	denotes	se
T3885	16410-16411	JJ	denotes	e
T3887	16411-16413	VBP	denotes	ct
T3886	16411-16414	sentence	denotes	cti
T3888	16413-16414	JJ	denotes	i
T3891	16414-16416	NN	denotes	on
T3889	16414-16546	sentence	denotes	ons stained with DiI and SYTOX green revealed ultrastructural differences between alphaA/BKO lenses and wild type lenses (Figure 3).
T3892	16416-16417	NNS	denotes	s
T3893	16418-16425	VBN	denotes	stained
T3894	16426-16430	IN	denotes	with
T3895	16431-16434	NN	denotes	DiI
T3896	16435-16438	CC	denotes	and
T3897	16439-16444	NN	denotes	SYTOX
T3898	16445-16450	NN	denotes	green
T3890	16451-16459	VBD	denotes	revealed
T3899	16460-16475	JJ	denotes	ultrastructural
T3900	16476-16487	NNS	denotes	differences
T3901	16488-16495	IN	denotes	between
T3902	16496-16502	NN	denotes	alphaA
T3904	16502-16503	HYPH	denotes	/
T3903	16503-16506	NN	denotes	BKO
T3905	16507-16513	NNS	denotes	lenses
T3906	16514-16517	CC	denotes	and
T3907	16518-16522	JJ	denotes	wild
T3908	16523-16527	NN	denotes	type
T3909	16528-16534	NNS	denotes	lenses
T3910	16535-16536	-LRB-	denotes	(
T3911	16536-16542	NN	denotes	Figure
T3912	16543-16544	CD	denotes	3
T3913	16544-16545	-RRB-	denotes	)
T3914	16545-16546	.	denotes	.
T3915	16546-16716	sentence	denotes	Anterior epithelial staining in 5 wk old alphaA/BKO (Figure 3A) and 5 wk old wild type lenses were similar (Figure 3K) with respect to nuclear staining with SYTOX green.
T3916	16547-16555	JJ	denotes	Anterior
T3918	16556-16566	JJ	denotes	epithelial
T3917	16567-16575	NN	denotes	staining
T3920	16576-16578	IN	denotes	in
T3921	16579-16580	CD	denotes	5
T3922	16581-16583	NN	denotes	wk
T3923	16584-16587	JJ	denotes	old
T3925	16588-16594	NN	denotes	alphaA
T3926	16594-16595	HYPH	denotes	/
T3924	16595-16598	NN	denotes	BKO
T3927	16599-16600	-LRB-	denotes	(
T3929	16600-16606	NN	denotes	Figure
T3928	16607-16609	NN	denotes	3A
T3930	16609-16610	-RRB-	denotes	)
T3931	16611-16614	CC	denotes	and
T3932	16615-16616	CD	denotes	5
T3933	16617-16619	NN	denotes	wk
T3934	16620-16623	JJ	denotes	old
T3936	16624-16628	JJ	denotes	wild
T3935	16629-16633	NN	denotes	type
T3937	16634-16640	NNS	denotes	lenses
T3919	16641-16645	VBD	denotes	were
T3938	16646-16653	JJ	denotes	similar
T3939	16654-16655	-LRB-	denotes	(
T3941	16655-16661	NN	denotes	Figure
T3940	16662-16664	NN	denotes	3K
T3942	16664-16665	-RRB-	denotes	)
T3943	16666-16670	IN	denotes	with
T3944	16671-16678	NN	denotes	respect
T3945	16679-16681	IN	denotes	to
T3946	16682-16689	JJ	denotes	nuclear
T3947	16690-16698	NN	denotes	staining
T3948	16699-16703	IN	denotes	with
T3949	16704-16709	NN	denotes	SYTOX
T3950	16710-16715	NN	denotes	green
T3951	16715-16716	.	denotes	.
T3952	16716-16928	sentence	denotes	However, some differences in 54 wk old alphaA/BKO lenses (Figure 3F) were observed in the central anterior epithelium compared to 5 wk (Figure 3K), 46 wk (Figure 3P), and 72 wk (data not shown) wild type lenses.
T3953	16717-16724	RB	denotes	However
T3955	16724-16726	,	denotes	,
T3956	16726-16730	DT	denotes	some
T3957	16731-16742	NNS	denotes	differences
T3958	16743-16745	IN	denotes	in
T3959	16746-16748	CD	denotes	54
T3960	16749-16751	NN	denotes	wk
T3961	16752-16755	JJ	denotes	old
T3963	16756-16762	NN	denotes	alphaA
T3965	16762-16763	HYPH	denotes	/
T3964	16763-16766	NN	denotes	BKO
T3962	16767-16773	NNS	denotes	lenses
T3966	16774-16775	-LRB-	denotes	(
T3968	16775-16781	NN	denotes	Figure
T3967	16782-16784	NN	denotes	3F
T3969	16784-16785	-RRB-	denotes	)
T3970	16786-16790	VBD	denotes	were
T3954	16791-16799	VBN	denotes	observed
T3971	16800-16802	IN	denotes	in
T3972	16803-16806	DT	denotes	the
T3974	16807-16814	JJ	denotes	central
T3975	16815-16823	JJ	denotes	anterior
T3973	16824-16834	NN	denotes	epithelium
T3976	16835-16843	VBN	denotes	compared
T3977	16844-16846	IN	denotes	to
T3978	16847-16848	CD	denotes	5
T3979	16849-16851	NN	denotes	wk
T3981	16852-16853	-LRB-	denotes	(
T3983	16853-16859	NN	denotes	Figure
T3982	16860-16862	NN	denotes	3K
T3984	16862-16863	-RRB-	denotes	)
T3985	16863-16865	,	denotes	,
T3986	16865-16867	CD	denotes	46
T3987	16868-16870	NN	denotes	wk
T3988	16871-16872	-LRB-	denotes	(
T3990	16872-16878	NN	denotes	Figure
T3989	16879-16881	NN	denotes	3P
T3991	16881-16882	-RRB-	denotes	)
T3992	16882-16884	,	denotes	,
T3993	16884-16887	CC	denotes	and
T3994	16888-16890	CD	denotes	72
T3995	16891-16893	NN	denotes	wk
T3996	16894-16895	-LRB-	denotes	(
T3998	16895-16899	NNS	denotes	data
T3999	16900-16903	RB	denotes	not
T3997	16904-16909	VBN	denotes	shown
T4000	16909-16910	-RRB-	denotes	)
T4001	16911-16915	JJ	denotes	wild
T4002	16916-16920	NN	denotes	type
T3980	16921-16927	NNS	denotes	lenses
T4003	16927-16928	.	denotes	.
T4004	16928-17058	sentence	denotes	These differences included changes in central epithelial nuclear staining, nuclear shape and epithelial thickness and continuity.
T4005	16929-16934	DT	denotes	These
T4006	16935-16946	NNS	denotes	differences
T4007	16947-16955	VBD	denotes	included
T4008	16956-16963	NNS	denotes	changes
T4009	16964-16966	IN	denotes	in
T4010	16967-16974	JJ	denotes	central
T4012	16975-16985	JJ	denotes	epithelial
T4013	16986-16993	JJ	denotes	nuclear
T4011	16994-17002	NN	denotes	staining
T4014	17002-17004	,	denotes	,
T4015	17004-17011	JJ	denotes	nuclear
T4016	17012-17017	NN	denotes	shape
T4017	17018-17021	CC	denotes	and
T4018	17022-17032	JJ	denotes	epithelial
T4019	17033-17042	NN	denotes	thickness
T4020	17043-17046	CC	denotes	and
T4021	17047-17057	NN	denotes	continuity
T4022	17057-17058	.	denotes	.
T4023	17058-17219	sentence	denotes	Superficial and deep anterior cortical staining was grossly different between alphaA/BKO (Figure 3A,3D,3F and 3I) and wild type lenses (Figure 3K,3N,3P and 3S).
T4024	17059-17070	JJ	denotes	Superficial
T4026	17071-17074	CC	denotes	and
T4027	17075-17079	JJ	denotes	deep
T4028	17080-17088	JJ	denotes	anterior
T4029	17089-17097	JJ	denotes	cortical
T4025	17098-17106	NN	denotes	staining
T4030	17107-17110	VBD	denotes	was
T4031	17111-17118	RB	denotes	grossly
T4032	17119-17128	JJ	denotes	different
T4033	17129-17136	IN	denotes	between
T4034	17137-17143	NN	denotes	alphaA
T4036	17143-17144	HYPH	denotes	/
T4035	17144-17147	NN	denotes	BKO
T4038	17148-17149	-LRB-	denotes	(
T4040	17149-17155	NN	denotes	Figure
T4039	17156-17158	NN	denotes	3A
T4041	17158-17159	,	denotes	,
T4042	17159-17161	NN	denotes	3D
T4043	17161-17162	,	denotes	,
T4044	17162-17164	NN	denotes	3F
T4045	17165-17168	CC	denotes	and
T4046	17169-17171	NN	denotes	3I
T4047	17171-17172	-RRB-	denotes	)
T4048	17173-17176	CC	denotes	and
T4049	17177-17181	JJ	denotes	wild
T4050	17182-17186	NN	denotes	type
T4037	17187-17193	NNS	denotes	lenses
T4051	17194-17195	-LRB-	denotes	(
T4053	17195-17201	NN	denotes	Figure
T4052	17202-17204	NN	denotes	3K
T4054	17204-17205	,	denotes	,
T4055	17205-17207	NN	denotes	3N
T4056	17207-17208	,	denotes	,
T4057	17208-17210	NN	denotes	3P
T4058	17211-17214	CC	denotes	and
T4059	17215-17217	NN	denotes	3S
T4060	17217-17218	-RRB-	denotes	)
T4061	17218-17219	.	denotes	.
T4062	17219-17431	sentence	denotes	In 5 and 54 wk old alphaA/BKO lenses, superficial and deep anterior cortical regions, stained for lipid membranes, did not reveal any patterns typical of fiber cells cut in cross-section or longitudinal section.
T4063	17220-17222	IN	denotes	In
T4065	17223-17224	CD	denotes	5
T4067	17225-17228	CC	denotes	and
T4068	17229-17231	CD	denotes	54
T4066	17232-17234	NN	denotes	wk
T4069	17235-17238	JJ	denotes	old
T4071	17239-17245	NN	denotes	alphaA
T4073	17245-17246	HYPH	denotes	/
T4072	17246-17249	NN	denotes	BKO
T4070	17250-17256	NNS	denotes	lenses
T4074	17256-17258	,	denotes	,
T4075	17258-17269	JJ	denotes	superficial
T4077	17270-17273	CC	denotes	and
T4078	17274-17278	JJ	denotes	deep
T4079	17279-17287	JJ	denotes	anterior
T4080	17288-17296	JJ	denotes	cortical
T4076	17297-17304	NNS	denotes	regions
T4081	17304-17306	,	denotes	,
T4082	17306-17313	VBN	denotes	stained
T4083	17314-17317	IN	denotes	for
T4084	17318-17323	NN	denotes	lipid
T4085	17324-17333	NNS	denotes	membranes
T4086	17333-17335	,	denotes	,
T4087	17335-17338	VBD	denotes	did
T4088	17339-17342	RB	denotes	not
T4064	17343-17349	VB	denotes	reveal
T4089	17350-17353	DT	denotes	any
T4090	17354-17362	NNS	denotes	patterns
T4091	17363-17370	JJ	denotes	typical
T4092	17371-17373	IN	denotes	of
T4093	17374-17379	NN	denotes	fiber
T4094	17380-17385	NNS	denotes	cells
T4095	17386-17389	VBN	denotes	cut
T4096	17390-17392	IN	denotes	in
T4097	17393-17406	NN	denotes	cross-section
T4098	17407-17409	CC	denotes	or
T4099	17410-17422	JJ	denotes	longitudinal
T4100	17423-17430	NN	denotes	section
T4101	17430-17431	.	denotes	.
T4102	17431-17588	sentence	denotes	In 5 wk alphaA/BKO lenses there was a high density of stained membranes per unit area throughout the cortex, with no nucleic acid staining in these regions.
T4103	17432-17434	IN	denotes	In
T4105	17435-17436	CD	denotes	5
T4106	17437-17439	NN	denotes	wk
T4108	17440-17446	NN	denotes	alphaA
T4110	17446-17447	HYPH	denotes	/
T4109	17447-17450	NN	denotes	BKO
T4107	17451-17457	NNS	denotes	lenses
T4111	17458-17463	EX	denotes	there
T4104	17464-17467	VBD	denotes	was
T4112	17468-17469	DT	denotes	a
T4114	17470-17474	JJ	denotes	high
T4113	17475-17482	NN	denotes	density
T4115	17483-17485	IN	denotes	of
T4116	17486-17493	VBN	denotes	stained
T4117	17494-17503	NNS	denotes	membranes
T4118	17504-17507	IN	denotes	per
T4119	17508-17512	NN	denotes	unit
T4120	17513-17517	NN	denotes	area
T4121	17518-17528	IN	denotes	throughout
T4122	17529-17532	DT	denotes	the
T4123	17533-17539	NN	denotes	cortex
T4124	17539-17541	,	denotes	,
T4125	17541-17545	IN	denotes	with
T4126	17546-17548	DT	denotes	no
T4128	17549-17556	JJ	denotes	nucleic
T4129	17557-17561	NN	denotes	acid
T4127	17562-17570	NN	denotes	staining
T4130	17571-17573	IN	denotes	in
T4131	17574-17579	DT	denotes	these
T4132	17580-17587	NNS	denotes	regions
T4133	17587-17588	.	denotes	.
T4134	17588-17739	sentence	denotes	In 54 wk alphaA/BKO lenses, large, irregularly shaped cells were observed, interspersed among regions of high membrane staining density per unit area.
T4135	17589-17591	IN	denotes	In
T4137	17592-17594	CD	denotes	54
T4138	17595-17597	NN	denotes	wk
T4140	17598-17604	NN	denotes	alphaA
T4142	17604-17605	HYPH	denotes	/
T4141	17605-17608	NN	denotes	BKO
T4139	17609-17615	NNS	denotes	lenses
T4143	17615-17617	,	denotes	,
T4144	17617-17622	JJ	denotes	large
T4146	17622-17624	,	denotes	,
T4147	17624-17635	RB	denotes	irregularly
T4148	17636-17642	VBN	denotes	shaped
T4145	17643-17648	NNS	denotes	cells
T4149	17649-17653	VBD	denotes	were
T4136	17654-17662	VBN	denotes	observed
T4150	17662-17664	,	denotes	,
T4151	17664-17676	JJ	denotes	interspersed
T4152	17677-17682	IN	denotes	among
T4153	17683-17690	NNS	denotes	regions
T4154	17691-17693	IN	denotes	of
T4155	17694-17698	JJ	denotes	high
T4157	17699-17707	NN	denotes	membrane
T4158	17708-17716	NN	denotes	staining
T4156	17717-17724	NN	denotes	density
T4159	17725-17728	IN	denotes	per
T4160	17729-17733	NN	denotes	unit
T4161	17734-17738	NN	denotes	area
T4162	17738-17739	.	denotes	.
T4163	17739-17954	sentence	denotes	These large objects were not vacuoles, because examination of the interior of these structures by transmitted and reflected light microscopy showed that the membranes encompassed cellular material (data not shown).
T4164	17740-17745	DT	denotes	These
T4166	17746-17751	JJ	denotes	large
T4165	17752-17759	NNS	denotes	objects
T4167	17760-17764	VBD	denotes	were
T4168	17765-17768	RB	denotes	not
T4169	17769-17777	NNS	denotes	vacuoles
T4170	17777-17779	,	denotes	,
T4171	17779-17786	IN	denotes	because
T4173	17787-17798	NN	denotes	examination
T4174	17799-17801	IN	denotes	of
T4175	17802-17805	DT	denotes	the
T4176	17806-17814	NN	denotes	interior
T4177	17815-17817	IN	denotes	of
T4178	17818-17823	DT	denotes	these
T4179	17824-17834	NNS	denotes	structures
T4180	17835-17837	IN	denotes	by
T4181	17838-17849	VBN	denotes	transmitted
T4183	17850-17853	CC	denotes	and
T4184	17854-17863	VBN	denotes	reflected
T4185	17864-17869	JJ	denotes	light
T4182	17870-17880	NN	denotes	microscopy
T4172	17881-17887	VBD	denotes	showed
T4186	17888-17892	IN	denotes	that
T4188	17893-17896	DT	denotes	the
T4189	17897-17906	NNS	denotes	membranes
T4187	17907-17918	VBD	denotes	encompassed
T4190	17919-17927	JJ	denotes	cellular
T4191	17928-17936	NN	denotes	material
T4192	17937-17938	-LRB-	denotes	(
T4194	17938-17942	NNS	denotes	data
T4195	17943-17946	RB	denotes	not
T4193	17947-17952	VBN	denotes	shown
T4196	17952-17953	-RRB-	denotes	)
T4197	17953-17954	.	denotes	.
T4198	17954-18091	sentence	denotes	In addition, nucleic acid staining was observed (Figure 3F and 3I) within many of these cells exhibiting large cross-sectional profiles.
T4199	17955-17957	IN	denotes	In
T4201	17958-17966	NN	denotes	addition
T4202	17966-17968	,	denotes	,
T4203	17968-17975	JJ	denotes	nucleic
T4204	17976-17980	NN	denotes	acid
T4205	17981-17989	NN	denotes	staining
T4206	17990-17993	VBD	denotes	was
T4200	17994-18002	VBN	denotes	observed
T4207	18003-18004	-LRB-	denotes	(
T4209	18004-18010	NN	denotes	Figure
T4208	18011-18013	NN	denotes	3F
T4210	18014-18017	CC	denotes	and
T4211	18018-18020	NN	denotes	3I
T4212	18020-18021	-RRB-	denotes	)
T4213	18022-18028	IN	denotes	within
T4214	18029-18033	JJ	denotes	many
T4215	18034-18036	IN	denotes	of
T4216	18037-18042	DT	denotes	these
T4217	18043-18048	NNS	denotes	cells
T4218	18049-18059	VBG	denotes	exhibiting
T4219	18060-18065	JJ	denotes	large
T4220	18066-18081	JJ	denotes	cross-sectional
T4221	18082-18090	NNS	denotes	profiles
T4222	18090-18091	.	denotes	.
T4223	18091-18232	sentence	denotes	In the wild type lenses, typical cross-sectional patterns of fiber cells organized in radial columns were observed (Figure 3K,3N,3P and 3S).
T4224	18092-18094	IN	denotes	In
T4226	18095-18098	DT	denotes	the
T4228	18099-18103	JJ	denotes	wild
T4229	18104-18108	NN	denotes	type
T4227	18109-18115	NNS	denotes	lenses
T4230	18115-18117	,	denotes	,
T4231	18117-18124	JJ	denotes	typical
T4233	18125-18140	JJ	denotes	cross-sectional
T4232	18141-18149	NNS	denotes	patterns
T4234	18150-18152	IN	denotes	of
T4235	18153-18158	NN	denotes	fiber
T4236	18159-18164	NNS	denotes	cells
T4237	18165-18174	VBN	denotes	organized
T4238	18175-18177	IN	denotes	in
T4239	18178-18184	JJ	denotes	radial
T4240	18185-18192	NNS	denotes	columns
T4241	18193-18197	VBD	denotes	were
T4225	18198-18206	VBN	denotes	observed
T4242	18207-18208	-LRB-	denotes	(
T4244	18208-18214	NN	denotes	Figure
T4243	18215-18217	NN	denotes	3K
T4245	18217-18218	,	denotes	,
T4246	18218-18220	NN	denotes	3N
T4247	18220-18221	,	denotes	,
T4248	18221-18223	NN	denotes	3P
T4249	18224-18227	CC	denotes	and
T4250	18228-18230	NN	denotes	3S
T4251	18230-18231	-RRB-	denotes	)
T4252	18231-18232	.	denotes	.
T4253	18232-18394	sentence	denotes	Ultrastructural differences at the equatorial/bow region were also observed between alphaA/BKO lenses (Figure 3B and 3G) and wild type lenses (Figure 3L and 3Q).
T4254	18233-18248	JJ	denotes	Ultrastructural
T4255	18249-18260	NNS	denotes	differences
T4257	18261-18263	IN	denotes	at
T4258	18264-18267	DT	denotes	the
T4260	18268-18278	JJ	denotes	equatorial
T4262	18278-18279	HYPH	denotes	/
T4261	18279-18282	JJ	denotes	bow
T4259	18283-18289	NN	denotes	region
T4263	18290-18294	VBD	denotes	were
T4264	18295-18299	RB	denotes	also
T4256	18300-18308	VBN	denotes	observed
T4265	18309-18316	IN	denotes	between
T4266	18317-18323	NN	denotes	alphaA
T4268	18323-18324	HYPH	denotes	/
T4267	18324-18327	NN	denotes	BKO
T4269	18328-18334	NNS	denotes	lenses
T4270	18335-18336	-LRB-	denotes	(
T4272	18336-18342	NN	denotes	Figure
T4271	18343-18345	NN	denotes	3B
T4273	18346-18349	CC	denotes	and
T4274	18350-18352	NN	denotes	3G
T4275	18352-18353	-RRB-	denotes	)
T4276	18354-18357	CC	denotes	and
T4277	18358-18362	JJ	denotes	wild
T4278	18363-18367	NN	denotes	type
T4279	18368-18374	NNS	denotes	lenses
T4280	18375-18376	-LRB-	denotes	(
T4282	18376-18382	NN	denotes	Figure
T4281	18383-18385	NN	denotes	3L
T4283	18386-18389	CC	denotes	and
T4284	18390-18392	NN	denotes	3Q
T4285	18392-18393	-RRB-	denotes	)
T4286	18393-18394	.	denotes	.
T4287	18394-18745	sentence	denotes	In contrast to wild type lenses (Figure 3L and 3Q), the alphaA/BKO lenses (Figure 3B and 3G) contained large areas devoid of cellular material, their nuclei were not limited to a well defined equatorial/bow region, and ordered radial columns of elongating fiber cells extending from the posterior capsule to the anterior epithelium were not observed.
T4288	18395-18397	IN	denotes	In
T4290	18398-18406	NN	denotes	contrast
T4291	18407-18409	IN	denotes	to
T4292	18410-18414	JJ	denotes	wild
T4293	18415-18419	NN	denotes	type
T4294	18420-18426	NNS	denotes	lenses
T4295	18427-18428	-LRB-	denotes	(
T4297	18428-18434	NN	denotes	Figure
T4296	18435-18437	NN	denotes	3L
T4298	18438-18441	CC	denotes	and
T4299	18442-18444	NN	denotes	3Q
T4300	18444-18445	-RRB-	denotes	)
T4301	18445-18447	,	denotes	,
T4302	18447-18450	DT	denotes	the
T4304	18451-18457	NN	denotes	alphaA
T4306	18457-18458	HYPH	denotes	/
T4305	18458-18461	NN	denotes	BKO
T4303	18462-18468	NNS	denotes	lenses
T4307	18469-18470	-LRB-	denotes	(
T4309	18470-18476	NN	denotes	Figure
T4308	18477-18479	NN	denotes	3B
T4310	18480-18483	CC	denotes	and
T4311	18484-18486	NN	denotes	3G
T4312	18486-18487	-RRB-	denotes	)
T4289	18488-18497	VBD	denotes	contained
T4313	18498-18503	JJ	denotes	large
T4314	18504-18509	NNS	denotes	areas
T4315	18510-18516	JJ	denotes	devoid
T4316	18517-18519	IN	denotes	of
T4317	18520-18528	JJ	denotes	cellular
T4318	18529-18537	NN	denotes	material
T4319	18537-18539	,	denotes	,
T4320	18539-18544	PRP$	denotes	their
T4321	18545-18551	NNS	denotes	nuclei
T4323	18552-18556	VBD	denotes	were
T4324	18557-18560	RB	denotes	not
T4322	18561-18568	VBN	denotes	limited
T4325	18569-18571	IN	denotes	to
T4326	18572-18573	DT	denotes	a
T4328	18574-18578	RB	denotes	well
T4329	18579-18586	VBN	denotes	defined
T4330	18587-18597	HYPH	denotes	equatorial
T4332	18597-18598	HYPH	denotes	/
T4331	18598-18601	JJ	denotes	bow
T4327	18602-18608	NN	denotes	region
T4333	18608-18610	,	denotes	,
T4334	18610-18613	CC	denotes	and
T4335	18614-18621	VBN	denotes	ordered
T4337	18622-18628	JJ	denotes	radial
T4336	18629-18636	NNS	denotes	columns
T4339	18637-18639	IN	denotes	of
T4340	18640-18650	VBG	denotes	elongating
T4342	18651-18656	NN	denotes	fiber
T4341	18657-18662	NNS	denotes	cells
T4343	18663-18672	VBG	denotes	extending
T4344	18673-18677	IN	denotes	from
T4345	18678-18681	DT	denotes	the
T4347	18682-18691	JJ	denotes	posterior
T4346	18692-18699	NN	denotes	capsule
T4348	18700-18702	IN	denotes	to
T4349	18703-18706	DT	denotes	the
T4351	18707-18715	JJ	denotes	anterior
T4350	18716-18726	NN	denotes	epithelium
T4352	18727-18731	VBD	denotes	were
T4353	18732-18735	RB	denotes	not
T4338	18736-18744	VBN	denotes	observed
T4354	18744-18745	.	denotes	.
T4355	18745-18961	sentence	denotes	In addition to the equatorial region, ultrastructural differences between alphaA/BKO lenses (Figure 3C and 3H) and wild type lenses (Figure 3M and 3R) were observed in fiber cells of the embryonic and fetal nucleus.
T4356	18746-18748	IN	denotes	In
T4358	18749-18757	NN	denotes	addition
T4359	18758-18760	IN	denotes	to
T4360	18761-18764	DT	denotes	the
T4362	18765-18775	JJ	denotes	equatorial
T4361	18776-18782	NN	denotes	region
T4363	18782-18784	,	denotes	,
T4364	18784-18799	JJ	denotes	ultrastructural
T4365	18800-18811	NNS	denotes	differences
T4366	18812-18819	IN	denotes	between
T4367	18820-18826	NN	denotes	alphaA
T4369	18826-18827	HYPH	denotes	/
T4368	18827-18830	NN	denotes	BKO
T4370	18831-18837	NNS	denotes	lenses
T4371	18838-18839	-LRB-	denotes	(
T4373	18839-18845	NN	denotes	Figure
T4372	18846-18848	NN	denotes	3C
T4374	18849-18852	CC	denotes	and
T4375	18853-18855	NN	denotes	3H
T4376	18855-18856	-RRB-	denotes	)
T4377	18857-18860	CC	denotes	and
T4378	18861-18865	JJ	denotes	wild
T4379	18866-18870	NN	denotes	type
T4380	18871-18877	NNS	denotes	lenses
T4381	18878-18879	-LRB-	denotes	(
T4383	18879-18885	NN	denotes	Figure
T4382	18886-18888	NN	denotes	3M
T4384	18889-18892	CC	denotes	and
T4385	18893-18895	NN	denotes	3R
T4386	18895-18896	-RRB-	denotes	)
T4387	18897-18901	VBD	denotes	were
T4357	18902-18910	VBN	denotes	observed
T4388	18911-18913	IN	denotes	in
T4389	18914-18919	NN	denotes	fiber
T4390	18920-18925	NNS	denotes	cells
T4391	18926-18928	IN	denotes	of
T4392	18929-18932	DT	denotes	the
T4394	18933-18942	JJ	denotes	embryonic
T4395	18943-18946	CC	denotes	and
T4396	18947-18952	JJ	denotes	fetal
T4393	18953-18960	NN	denotes	nucleus
T4397	18960-18961	.	denotes	.
T4398	18961-19295	sentence	denotes	In 5 wk alphaA/BKO lenses (Figure 3C), a greater variation in cross sectional diameter was observed, with cell diameters ranging from less than 2 microns up to approximately 25 microns, compared to the 5 wk and 46 wk wild type lenses, whose diameters ranged from less than 2 microns up to approximately 10 microns (Figure 3M and 3R).
T4399	18962-18964	IN	denotes	In
T4401	18965-18966	CD	denotes	5
T4402	18967-18969	NN	denotes	wk
T4404	18970-18976	NN	denotes	alphaA
T4406	18976-18977	HYPH	denotes	/
T4405	18977-18980	NN	denotes	BKO
T4403	18981-18987	NNS	denotes	lenses
T4407	18988-18989	-LRB-	denotes	(
T4409	18989-18995	NN	denotes	Figure
T4408	18996-18998	NN	denotes	3C
T4410	18998-18999	-RRB-	denotes	)
T4411	18999-19001	,	denotes	,
T4412	19001-19002	DT	denotes	a
T4414	19003-19010	JJR	denotes	greater
T4413	19011-19020	NN	denotes	variation
T4415	19021-19023	IN	denotes	in
T4416	19024-19029	AFX	denotes	cross
T4417	19030-19039	JJ	denotes	sectional
T4418	19040-19048	NN	denotes	diameter
T4419	19049-19052	VBD	denotes	was
T4400	19053-19061	VBN	denotes	observed
T4420	19061-19063	,	denotes	,
T4421	19063-19067	IN	denotes	with
T4422	19068-19072	NN	denotes	cell
T4423	19073-19082	NNS	denotes	diameters
T4424	19083-19090	VBG	denotes	ranging
T4425	19091-19095	IN	denotes	from
T4426	19096-19100	JJR	denotes	less
T4428	19101-19105	IN	denotes	than
T4427	19106-19107	CD	denotes	2
T4429	19108-19115	NNS	denotes	microns
T4430	19116-19118	RB	denotes	up
T4431	19119-19121	IN	denotes	to
T4432	19122-19135	RB	denotes	approximately
T4433	19136-19138	CD	denotes	25
T4434	19139-19146	NNS	denotes	microns
T4435	19146-19148	,	denotes	,
T4436	19148-19156	VBN	denotes	compared
T4437	19157-19159	IN	denotes	to
T4438	19160-19163	DT	denotes	the
T4440	19164-19165	CD	denotes	5
T4441	19166-19168	NN	denotes	wk
T4442	19169-19172	CC	denotes	and
T4443	19173-19175	CD	denotes	46
T4444	19176-19178	NN	denotes	wk
T4445	19179-19183	JJ	denotes	wild
T4446	19184-19188	NN	denotes	type
T4439	19189-19195	NNS	denotes	lenses
T4447	19195-19197	,	denotes	,
T4448	19197-19202	WP$	denotes	whose
T4449	19203-19212	NNS	denotes	diameters
T4450	19213-19219	VBD	denotes	ranged
T4451	19220-19224	IN	denotes	from
T4452	19225-19229	JJR	denotes	less
T4454	19230-19234	IN	denotes	than
T4453	19235-19236	CD	denotes	2
T4455	19237-19244	NNS	denotes	microns
T4456	19245-19247	RB	denotes	up
T4457	19248-19250	IN	denotes	to
T4458	19251-19264	RB	denotes	approximately
T4459	19265-19267	CD	denotes	10
T4460	19268-19275	NNS	denotes	microns
T4461	19276-19277	-LRB-	denotes	(
T4463	19277-19283	NN	denotes	Figure
T4462	19284-19286	NN	denotes	3M
T4464	19287-19290	CC	denotes	and
T4465	19291-19293	NN	denotes	3R
T4466	19293-19294	-RRB-	denotes	)
T4467	19294-19295	.	denotes	.
T4468	19295-19481	sentence	denotes	At 54 wk, these cells in the alphaA/BKO lenses were larger in cross sectional diameter than wild type, and exhibited a much greater variation in the cross sectional size and cell shape.
T4469	19296-19298	IN	denotes	At
T4471	19299-19301	CD	denotes	54
T4472	19302-19304	NNS	denotes	wk
T4473	19304-19306	,	denotes	,
T4474	19306-19311	DT	denotes	these
T4475	19312-19317	NNS	denotes	cells
T4476	19318-19320	IN	denotes	in
T4477	19321-19324	DT	denotes	the
T4479	19325-19331	NN	denotes	alphaA
T4481	19331-19332	HYPH	denotes	/
T4480	19332-19335	NN	denotes	BKO
T4478	19336-19342	NNS	denotes	lenses
T4470	19343-19347	VBD	denotes	were
T4482	19348-19354	JJR	denotes	larger
T4483	19355-19357	IN	denotes	in
T4484	19358-19363	JJ	denotes	cross
T4485	19364-19373	JJ	denotes	sectional
T4486	19374-19382	NN	denotes	diameter
T4487	19383-19387	IN	denotes	than
T4488	19388-19392	JJ	denotes	wild
T4489	19393-19397	NN	denotes	type
T4490	19397-19399	,	denotes	,
T4491	19399-19402	CC	denotes	and
T4492	19403-19412	VBD	denotes	exhibited
T4493	19413-19414	DT	denotes	a
T4495	19415-19419	RB	denotes	much
T4496	19420-19427	JJR	denotes	greater
T4494	19428-19437	NN	denotes	variation
T4497	19438-19440	IN	denotes	in
T4498	19441-19444	DT	denotes	the
T4500	19445-19450	AFX	denotes	cross
T4501	19451-19460	JJ	denotes	sectional
T4499	19461-19465	NN	denotes	size
T4502	19466-19469	CC	denotes	and
T4503	19470-19474	NN	denotes	cell
T4504	19475-19480	NN	denotes	shape
T4505	19480-19481	.	denotes	.
T4506	19481-19658	sentence	denotes	Cells adjacent to the posterior capsule of 5 wk alphaA/BKO lenses (Figure 3E) exhibited nucleic acid staining, were small and non-elongated, with numerous membrane projections.
T4507	19482-19487	NNS	denotes	Cells
T4509	19488-19496	JJ	denotes	adjacent
T4510	19497-19499	IN	denotes	to
T4511	19500-19503	DT	denotes	the
T4513	19504-19513	JJ	denotes	posterior
T4512	19514-19521	NN	denotes	capsule
T4514	19522-19524	IN	denotes	of
T4515	19525-19526	CD	denotes	5
T4516	19527-19529	NN	denotes	wk
T4518	19530-19536	NN	denotes	alphaA
T4520	19536-19537	HYPH	denotes	/
T4519	19537-19540	NN	denotes	BKO
T4517	19541-19547	NNS	denotes	lenses
T4521	19548-19549	-LRB-	denotes	(
T4523	19549-19555	NN	denotes	Figure
T4522	19556-19558	NN	denotes	3E
T4524	19558-19559	-RRB-	denotes	)
T4508	19560-19569	VBD	denotes	exhibited
T4525	19570-19577	JJ	denotes	nucleic
T4526	19578-19582	NN	denotes	acid
T4527	19583-19591	NN	denotes	staining
T4528	19591-19593	,	denotes	,
T4529	19593-19597	VBD	denotes	were
T4530	19598-19603	JJ	denotes	small
T4531	19604-19607	CC	denotes	and
T4532	19608-19621	JJ	denotes	non-elongated
T4533	19621-19623	,	denotes	,
T4534	19623-19627	IN	denotes	with
T4535	19628-19636	JJ	denotes	numerous
T4537	19637-19645	NN	denotes	membrane
T4536	19646-19657	NNS	denotes	projections
T4538	19657-19658	.	denotes	.
T4539	19658-19660	,	denotes	,
T4541	19658-19721	sentence	denotes	, These were, however, not observed in 54 wk alphaA/BKO lenses.
T4542	19660-19665	DT	denotes	These
T4543	19666-19670	VBD	denotes	were
T4544	19670-19672	,	denotes	,
T4545	19672-19679	RB	denotes	however
T4546	19679-19681	,	denotes	,
T4547	19681-19684	RB	denotes	not
T4540	19685-19693	VBN	denotes	observed
T4548	19694-19696	IN	denotes	in
T4549	19697-19699	CD	denotes	54
T4550	19700-19702	NN	denotes	wk
T4552	19703-19709	NN	denotes	alphaA
T4554	19709-19710	HYPH	denotes	/
T4553	19710-19713	NN	denotes	BKO
T4551	19714-19720	NNS	denotes	lenses
T4555	19720-19721	.	denotes	.
T4556	19721-19891	sentence	denotes	In 5 wk alphaA/BKO lenses, deep to the posterior capsule, larger diameter cells, similar to those observed in the embryonic/fetal nucleus were observed (data not shown).
T4557	19722-19724	IN	denotes	In
T4559	19725-19726	CD	denotes	5
T4560	19727-19729	NN	denotes	wk
T4562	19730-19736	NN	denotes	alphaA
T4564	19736-19737	HYPH	denotes	/
T4563	19737-19740	NN	denotes	BKO
T4561	19741-19747	NNS	denotes	lenses
T4565	19747-19749	,	denotes	,
T4566	19749-19753	RB	denotes	deep
T4567	19754-19756	IN	denotes	to
T4568	19757-19760	DT	denotes	the
T4570	19761-19770	JJ	denotes	posterior
T4569	19771-19778	NN	denotes	capsule
T4571	19778-19780	,	denotes	,
T4572	19780-19786	JJR	denotes	larger
T4574	19787-19795	NN	denotes	diameter
T4573	19796-19801	NNS	denotes	cells
T4575	19801-19803	,	denotes	,
T4576	19803-19810	JJ	denotes	similar
T4577	19811-19813	IN	denotes	to
T4578	19814-19819	DT	denotes	those
T4579	19820-19828	VBN	denotes	observed
T4580	19829-19831	IN	denotes	in
T4581	19832-19835	DT	denotes	the
T4583	19836-19845	JJ	denotes	embryonic
T4585	19845-19846	HYPH	denotes	/
T4584	19846-19851	JJ	denotes	fetal
T4582	19852-19859	NN	denotes	nucleus
T4586	19860-19864	VBD	denotes	were
T4558	19865-19873	VBN	denotes	observed
T4587	19874-19875	-LRB-	denotes	(
T4589	19875-19879	NNS	denotes	data
T4590	19880-19883	RB	denotes	not
T4588	19884-19889	VBN	denotes	shown
T4591	19889-19890	-RRB-	denotes	)
T4592	19890-19891	.	denotes	.
T4593	19891-20048	sentence	denotes	Fiber cells of similar dimension and appearance to embryonic/fetal nuclear fibers were seen at the posterior capsule in 54 wk alphaA/BKO lenses (Figure 3J).
T4594	19892-19897	NN	denotes	Fiber
T4595	19898-19903	NNS	denotes	cells
T4597	19904-19906	IN	denotes	of
T4598	19907-19914	JJ	denotes	similar
T4599	19915-19924	NN	denotes	dimension
T4600	19925-19928	CC	denotes	and
T4601	19929-19939	NN	denotes	appearance
T4602	19940-19942	IN	denotes	to
T4603	19943-19952	JJ	denotes	embryonic
T4605	19952-19953	HYPH	denotes	/
T4604	19953-19958	JJ	denotes	fetal
T4607	19959-19966	JJ	denotes	nuclear
T4606	19967-19973	NNS	denotes	fibers
T4608	19974-19978	VBD	denotes	were
T4596	19979-19983	VBN	denotes	seen
T4609	19984-19986	IN	denotes	at
T4610	19987-19990	DT	denotes	the
T4612	19991-20000	JJ	denotes	posterior
T4611	20001-20008	NN	denotes	capsule
T4613	20009-20011	IN	denotes	in
T4614	20012-20014	CD	denotes	54
T4615	20015-20017	NN	denotes	wk
T4617	20018-20024	NN	denotes	alphaA
T4619	20024-20025	HYPH	denotes	/
T4618	20025-20028	NN	denotes	BKO
T4616	20029-20035	NNS	denotes	lenses
T4620	20036-20037	-LRB-	denotes	(
T4622	20037-20043	NN	denotes	Figure
T4621	20044-20046	NN	denotes	3J
T4623	20046-20047	-RRB-	denotes	)
T4624	20047-20048	.	denotes	.
T4625	20048-20179	sentence	denotes	In both 5 and 54 wk alphaA/BKO lenses, no posterior sutures could be found in any axial or equatorial vibratome sections examined.
T4626	20049-20051	IN	denotes	In
T4628	20052-20056	DT	denotes	both
T4629	20057-20058	CD	denotes	5
T4631	20059-20062	CC	denotes	and
T4632	20063-20065	CD	denotes	54
T4630	20066-20068	NN	denotes	wk
T4634	20069-20075	NN	denotes	alphaA
T4636	20075-20076	HYPH	denotes	/
T4635	20076-20079	NN	denotes	BKO
T4633	20080-20086	NNS	denotes	lenses
T4637	20086-20088	,	denotes	,
T4638	20088-20090	DT	denotes	no
T4640	20091-20100	JJ	denotes	posterior
T4639	20101-20108	NNS	denotes	sutures
T4641	20109-20114	MD	denotes	could
T4642	20115-20117	VB	denotes	be
T4627	20118-20123	VBN	denotes	found
T4643	20124-20126	IN	denotes	in
T4644	20127-20130	DT	denotes	any
T4646	20131-20136	JJ	denotes	axial
T4647	20137-20139	CC	denotes	or
T4648	20140-20150	JJ	denotes	equatorial
T4649	20151-20160	NN	denotes	vibratome
T4645	20161-20169	NNS	denotes	sections
T4650	20170-20178	VBN	denotes	examined
T4651	20178-20179	.	denotes	.
T4652	20179-20278	sentence	denotes	Posterior sutures were readily found in vibratome sections of wild type lenses (Figure 3O, arrow).
T4653	20180-20189	JJ	denotes	Posterior
T4654	20190-20197	NNS	denotes	sutures
T4656	20198-20202	VBD	denotes	were
T4657	20203-20210	RB	denotes	readily
T4655	20211-20216	VBN	denotes	found
T4658	20217-20219	IN	denotes	in
T4659	20220-20229	NN	denotes	vibratome
T4660	20230-20238	NNS	denotes	sections
T4661	20239-20241	IN	denotes	of
T4662	20242-20246	JJ	denotes	wild
T4663	20247-20251	NN	denotes	type
T4664	20252-20258	NNS	denotes	lenses
T4665	20259-20260	-LRB-	denotes	(
T4667	20260-20266	NN	denotes	Figure
T4668	20267-20269	CD	denotes	3O
T4669	20269-20271	,	denotes	,
T4666	20271-20276	NN	denotes	arrow
T4670	20276-20277	-RRB-	denotes	)
T4671	20277-20278	.	denotes	.
T4672	20278-20447	sentence	denotes	Fiber cells attached to the posterior capsule and extending anteriorly were observed in 5 wk (Figure 3O), 46 wk (Figure 3T) and 72 wk (data not shown) wild type lenses.
T4673	20279-20284	NN	denotes	Fiber
T4674	20285-20290	NNS	denotes	cells
T4676	20291-20299	VBN	denotes	attached
T4677	20300-20302	IN	denotes	to
T4678	20303-20306	DT	denotes	the
T4680	20307-20316	JJ	denotes	posterior
T4679	20317-20324	NN	denotes	capsule
T4681	20325-20328	CC	denotes	and
T4682	20329-20338	VBG	denotes	extending
T4683	20339-20349	RB	denotes	anteriorly
T4684	20350-20354	VBD	denotes	were
T4675	20355-20363	VBN	denotes	observed
T4685	20364-20366	IN	denotes	in
T4686	20367-20368	CD	denotes	5
T4687	20369-20371	NN	denotes	wk
T4689	20372-20373	-LRB-	denotes	(
T4691	20373-20379	NN	denotes	Figure
T4690	20380-20382	NN	denotes	3O
T4692	20382-20383	-RRB-	denotes	)
T4693	20383-20385	,	denotes	,
T4694	20385-20387	CD	denotes	46
T4695	20388-20390	NN	denotes	wk
T4696	20391-20392	-LRB-	denotes	(
T4698	20392-20398	NN	denotes	Figure
T4697	20399-20401	NN	denotes	3T
T4699	20401-20402	-RRB-	denotes	)
T4700	20403-20406	CC	denotes	and
T4701	20407-20409	CD	denotes	72
T4702	20410-20412	NN	denotes	wk
T4703	20413-20414	-LRB-	denotes	(
T4705	20414-20418	NNS	denotes	data
T4706	20419-20422	RB	denotes	not
T4704	20423-20428	VBN	denotes	shown
T4707	20428-20429	-RRB-	denotes	)
T4708	20430-20434	JJ	denotes	wild
T4709	20435-20439	NN	denotes	type
T4688	20440-20446	NNS	denotes	lenses
T4710	20446-20447	.	denotes	.
T4711	20447-20515	sentence	denotes	SEM confirmed many of the observations made by confocal microscopy.
T4712	20448-20451	NN	denotes	SEM
T4713	20452-20461	VBD	denotes	confirmed
T4714	20462-20466	JJ	denotes	many
T4715	20467-20469	IN	denotes	of
T4716	20470-20473	DT	denotes	the
T4717	20474-20486	NNS	denotes	observations
T4718	20487-20491	VBN	denotes	made
T4719	20492-20494	IN	denotes	by
T4720	20495-20503	JJ	denotes	confocal
T4721	20504-20514	NN	denotes	microscopy
T4722	20514-20515	.	denotes	.
T4723	20515-20880	sentence	denotes	In the equatorial/bow region, a disorganized array of relatively small irregular shaped cells were observed in 5 wk (Figure 4A) and 54 wk (Figure 4D) alphaA/BKO lenses, while elongated fiber cells organized into radial columns, with ball and socket interdigitations, were observed in 5 wk (Figure 4G), 46 wk (Figure 4J) and 72 wk (data not shown) wild type lenses.
T4724	20516-20518	IN	denotes	In
T4726	20519-20522	DT	denotes	the
T4728	20523-20533	JJ	denotes	equatorial
T4730	20533-20534	HYPH	denotes	/
T4729	20534-20537	JJ	denotes	bow
T4727	20538-20544	NN	denotes	region
T4731	20544-20546	,	denotes	,
T4732	20546-20547	DT	denotes	a
T4734	20548-20560	JJ	denotes	disorganized
T4733	20561-20566	NN	denotes	array
T4735	20567-20569	IN	denotes	of
T4736	20570-20580	RB	denotes	relatively
T4737	20581-20586	JJ	denotes	small
T4739	20587-20596	JJ	denotes	irregular
T4740	20597-20603	VBN	denotes	shaped
T4738	20604-20609	NNS	denotes	cells
T4741	20610-20614	VBD	denotes	were
T4725	20615-20623	VBN	denotes	observed
T4742	20624-20626	IN	denotes	in
T4743	20627-20628	CD	denotes	5
T4744	20629-20631	NN	denotes	wk
T4746	20632-20633	-LRB-	denotes	(
T4748	20633-20639	NN	denotes	Figure
T4747	20640-20642	NN	denotes	4A
T4749	20642-20643	-RRB-	denotes	)
T4750	20644-20647	CC	denotes	and
T4751	20648-20650	CD	denotes	54
T4752	20651-20653	NN	denotes	wk
T4753	20654-20655	-LRB-	denotes	(
T4755	20655-20661	NN	denotes	Figure
T4754	20662-20664	NN	denotes	4D
T4756	20664-20665	-RRB-	denotes	)
T4757	20666-20672	NN	denotes	alphaA
T4759	20672-20673	HYPH	denotes	/
T4758	20673-20676	NN	denotes	BKO
T4745	20677-20683	NNS	denotes	lenses
T4760	20683-20685	,	denotes	,
T4761	20685-20690	IN	denotes	while
T4763	20691-20700	VBN	denotes	elongated
T4765	20701-20706	NN	denotes	fiber
T4764	20707-20712	NNS	denotes	cells
T4766	20713-20722	VBN	denotes	organized
T4767	20723-20727	IN	denotes	into
T4768	20728-20734	JJ	denotes	radial
T4769	20735-20742	NNS	denotes	columns
T4770	20742-20744	,	denotes	,
T4771	20744-20748	IN	denotes	with
T4772	20749-20753	NN	denotes	ball
T4774	20754-20757	CC	denotes	and
T4775	20758-20764	NN	denotes	socket
T4773	20765-20781	NNS	denotes	interdigitations
T4776	20781-20783	,	denotes	,
T4777	20783-20787	VBD	denotes	were
T4762	20788-20796	VBN	denotes	observed
T4778	20797-20799	IN	denotes	in
T4779	20800-20801	CD	denotes	5
T4780	20802-20804	NN	denotes	wk
T4782	20805-20806	-LRB-	denotes	(
T4784	20806-20812	NN	denotes	Figure
T4783	20813-20815	NN	denotes	4G
T4785	20815-20816	-RRB-	denotes	)
T4786	20816-20818	,	denotes	,
T4787	20818-20820	CD	denotes	46
T4788	20821-20823	NN	denotes	wk
T4789	20824-20825	-LRB-	denotes	(
T4791	20825-20831	NN	denotes	Figure
T4790	20832-20834	NN	denotes	4J
T4792	20834-20835	-RRB-	denotes	)
T4793	20836-20839	CC	denotes	and
T4794	20840-20842	CD	denotes	72
T4795	20843-20845	NN	denotes	wk
T4796	20846-20847	-LRB-	denotes	(
T4798	20847-20851	NNS	denotes	data
T4799	20852-20855	RB	denotes	not
T4797	20856-20861	VBN	denotes	shown
T4800	20861-20862	-RRB-	denotes	)
T4801	20863-20867	JJ	denotes	wild
T4802	20868-20872	NN	denotes	type
T4781	20873-20879	NNS	denotes	lenses
T4803	20879-20880	.	denotes	.
T4804	20880-21109	sentence	denotes	In the anterior cortical region of 5 wk alphaA/BKO lenses, radial columns of cells with numerous cell surface projections were observed (Figure 4B), but these radial columns were no longer present at 54 weeks of age (Figure 4E).
T4805	20881-20883	IN	denotes	In
T4807	20884-20887	DT	denotes	the
T4809	20888-20896	JJ	denotes	anterior
T4810	20897-20905	JJ	denotes	cortical
T4808	20906-20912	NN	denotes	region
T4811	20913-20915	IN	denotes	of
T4812	20916-20917	CD	denotes	5
T4813	20918-20920	NN	denotes	wk
T4815	20921-20927	NN	denotes	alphaA
T4817	20927-20928	HYPH	denotes	/
T4816	20928-20931	NN	denotes	BKO
T4814	20932-20938	NNS	denotes	lenses
T4818	20938-20940	,	denotes	,
T4819	20940-20946	JJ	denotes	radial
T4820	20947-20954	NNS	denotes	columns
T4821	20955-20957	IN	denotes	of
T4822	20958-20963	NNS	denotes	cells
T4823	20964-20968	IN	denotes	with
T4824	20969-20977	JJ	denotes	numerous
T4826	20978-20982	NN	denotes	cell
T4827	20983-20990	NN	denotes	surface
T4825	20991-21002	NNS	denotes	projections
T4828	21003-21007	VBD	denotes	were
T4806	21008-21016	VBN	denotes	observed
T4829	21017-21018	-LRB-	denotes	(
T4831	21018-21024	NN	denotes	Figure
T4830	21025-21027	NN	denotes	4B
T4832	21027-21028	-RRB-	denotes	)
T4833	21028-21030	,	denotes	,
T4834	21030-21033	CC	denotes	but
T4835	21034-21039	DT	denotes	these
T4837	21040-21046	JJ	denotes	radial
T4836	21047-21054	NNS	denotes	columns
T4838	21055-21059	VBD	denotes	were
T4839	21060-21062	RB	denotes	no
T4840	21063-21069	RBR	denotes	longer
T4841	21070-21077	JJ	denotes	present
T4842	21078-21080	IN	denotes	at
T4843	21081-21083	CD	denotes	54
T4844	21084-21089	NNS	denotes	weeks
T4845	21090-21092	IN	denotes	of
T4846	21093-21096	NN	denotes	age
T4847	21097-21098	-LRB-	denotes	(
T4849	21098-21104	NN	denotes	Figure
T4848	21105-21107	NN	denotes	4E
T4850	21107-21108	-RRB-	denotes	)
T4851	21108-21109	.	denotes	.
T4852	21109-21230	sentence	denotes	In these older lenses, irregularly shaped, convoluted cells, with no recognizable pattern of organization, were present.
T4853	21110-21112	IN	denotes	In
T4855	21113-21118	DT	denotes	these
T4857	21119-21124	JJR	denotes	older
T4856	21125-21131	NNS	denotes	lenses
T4858	21131-21133	,	denotes	,
T4859	21133-21144	RB	denotes	irregularly
T4860	21145-21151	VBN	denotes	shaped
T4862	21151-21153	,	denotes	,
T4863	21153-21163	VBN	denotes	convoluted
T4861	21164-21169	NNS	denotes	cells
T4864	21169-21171	,	denotes	,
T4865	21171-21175	IN	denotes	with
T4866	21176-21178	DT	denotes	no
T4868	21179-21191	JJ	denotes	recognizable
T4867	21192-21199	NN	denotes	pattern
T4869	21200-21202	IN	denotes	of
T4870	21203-21215	NN	denotes	organization
T4871	21215-21217	,	denotes	,
T4854	21217-21221	VBD	denotes	were
T4872	21222-21229	JJ	denotes	present
T4873	21229-21230	.	denotes	.
T4874	21230-21480	sentence	denotes	In contrast, radial columns of elongated fiber cells of uniform size and shape, with ball and socket interdigitations, were present in the anterior cortical region of 5 wk (Figure 4H), 46 wk (Figure 4K) and 72 week (data not shown) wild type lenses.
T4875	21231-21233	IN	denotes	In
T4877	21234-21242	NN	denotes	contrast
T4878	21242-21244	,	denotes	,
T4879	21244-21250	JJ	denotes	radial
T4880	21251-21258	NNS	denotes	columns
T4881	21259-21261	IN	denotes	of
T4882	21262-21271	VBN	denotes	elongated
T4884	21272-21277	NN	denotes	fiber
T4883	21278-21283	NNS	denotes	cells
T4885	21284-21286	IN	denotes	of
T4886	21287-21294	JJ	denotes	uniform
T4887	21295-21299	NN	denotes	size
T4888	21300-21303	CC	denotes	and
T4889	21304-21309	NN	denotes	shape
T4890	21309-21311	,	denotes	,
T4891	21311-21315	IN	denotes	with
T4892	21316-21320	NN	denotes	ball
T4894	21321-21324	CC	denotes	and
T4895	21325-21331	NN	denotes	socket
T4893	21332-21348	NNS	denotes	interdigitations
T4896	21348-21350	,	denotes	,
T4876	21350-21354	VBD	denotes	were
T4897	21355-21362	JJ	denotes	present
T4898	21363-21365	IN	denotes	in
T4899	21366-21369	DT	denotes	the
T4901	21370-21378	JJ	denotes	anterior
T4902	21379-21387	JJ	denotes	cortical
T4900	21388-21394	NN	denotes	region
T4903	21395-21397	IN	denotes	of
T4904	21398-21399	CD	denotes	5
T4905	21400-21402	NN	denotes	wk
T4907	21403-21404	-LRB-	denotes	(
T4909	21404-21410	NN	denotes	Figure
T4908	21411-21413	NN	denotes	4H
T4910	21413-21414	-RRB-	denotes	)
T4911	21414-21416	,	denotes	,
T4912	21416-21418	CD	denotes	46
T4913	21419-21421	NN	denotes	wk
T4914	21422-21423	-LRB-	denotes	(
T4916	21423-21429	NN	denotes	Figure
T4915	21430-21432	NN	denotes	4K
T4917	21432-21433	-RRB-	denotes	)
T4918	21434-21437	CC	denotes	and
T4919	21438-21440	CD	denotes	72
T4920	21441-21445	NN	denotes	week
T4921	21446-21447	-LRB-	denotes	(
T4923	21447-21451	NNS	denotes	data
T4924	21452-21455	RB	denotes	not
T4922	21456-21461	VBN	denotes	shown
T4925	21461-21462	-RRB-	denotes	)
T4926	21463-21467	JJ	denotes	wild
T4927	21468-21472	NN	denotes	type
T4906	21473-21479	NNS	denotes	lenses
T4928	21479-21480	.	denotes	.
T4929	21480-21656	sentence	denotes	The posterior subcapsular regions of 5 wk (Figure 4C) and 54 wk (Figure 4F) alphaA/BKO lenses were filled with irregularly shaped cells with numerous cell surface projections.
T4930	21481-21484	DT	denotes	The
T4932	21485-21494	JJ	denotes	posterior
T4933	21495-21506	JJ	denotes	subcapsular
T4931	21507-21514	NNS	denotes	regions
T4935	21515-21517	IN	denotes	of
T4936	21518-21519	CD	denotes	5
T4937	21520-21522	NN	denotes	wk
T4939	21523-21524	-LRB-	denotes	(
T4941	21524-21530	NN	denotes	Figure
T4940	21531-21533	NN	denotes	4C
T4942	21533-21534	-RRB-	denotes	)
T4943	21535-21538	CC	denotes	and
T4944	21539-21541	CD	denotes	54
T4945	21542-21544	NN	denotes	wk
T4946	21545-21546	-LRB-	denotes	(
T4948	21546-21552	NN	denotes	Figure
T4947	21553-21555	NN	denotes	4F
T4949	21555-21556	-RRB-	denotes	)
T4950	21557-21563	NN	denotes	alphaA
T4952	21563-21564	HYPH	denotes	/
T4951	21564-21567	NN	denotes	BKO
T4938	21568-21574	NNS	denotes	lenses
T4953	21575-21579	VBD	denotes	were
T4934	21580-21586	VBN	denotes	filled
T4954	21587-21591	IN	denotes	with
T4955	21592-21603	RB	denotes	irregularly
T4956	21604-21610	VBN	denotes	shaped
T4957	21611-21616	NNS	denotes	cells
T4958	21617-21621	IN	denotes	with
T4959	21622-21630	JJ	denotes	numerous
T4961	21631-21635	NN	denotes	cell
T4962	21636-21643	NN	denotes	surface
T4960	21644-21655	NNS	denotes	projections
T4963	21655-21656	.	denotes	.
T4964	21656-21803	sentence	denotes	In the fetal/embryonic nucleus, elongated fiber cells with numerous long, finger-like projections and furrowed membranes were evident (not shown).
T4965	21657-21659	IN	denotes	In
T4967	21660-21663	DT	denotes	the
T4969	21664-21669	JJ	denotes	fetal
T4971	21669-21670	HYPH	denotes	/
T4970	21670-21679	JJ	denotes	embryonic
T4968	21680-21687	NN	denotes	nucleus
T4972	21687-21689	,	denotes	,
T4973	21689-21698	VBN	denotes	elongated
T4975	21699-21704	NN	denotes	fiber
T4974	21705-21710	NNS	denotes	cells
T4976	21711-21715	IN	denotes	with
T4977	21716-21724	JJ	denotes	numerous
T4979	21725-21729	JJ	denotes	long
T4980	21729-21731	,	denotes	,
T4981	21731-21737	JJ	denotes	finger
T4983	21737-21738	HYPH	denotes	-
T4982	21738-21742	JJ	denotes	like
T4978	21743-21754	NNS	denotes	projections
T4984	21755-21758	CC	denotes	and
T4985	21759-21767	VBN	denotes	furrowed
T4986	21768-21777	NNS	denotes	membranes
T4966	21778-21782	VBD	denotes	were
T4987	21783-21790	JJ	denotes	evident
T4988	21791-21792	-LRB-	denotes	(
T4990	21792-21795	RB	denotes	not
T4989	21796-21801	VBN	denotes	shown
T4991	21801-21802	-RRB-	denotes	)
T4992	21802-21803	.	denotes	.
T4993	21803-21913	sentence	denotes	No elongated cells extending from the bow region to the posterior capsule of alphaA/BKO lenses were observed.
T4994	21804-21806	DT	denotes	No
T4996	21807-21816	VBN	denotes	elongated
T4995	21817-21822	NNS	denotes	cells
T4998	21823-21832	VBG	denotes	extending
T4999	21833-21837	IN	denotes	from
T5000	21838-21841	DT	denotes	the
T5002	21842-21845	NN	denotes	bow
T5001	21846-21852	NN	denotes	region
T5003	21853-21855	IN	denotes	to
T5004	21856-21859	DT	denotes	the
T5006	21860-21869	JJ	denotes	posterior
T5005	21870-21877	NN	denotes	capsule
T5007	21878-21880	IN	denotes	of
T5008	21881-21887	NN	denotes	alphaA
T5010	21887-21888	HYPH	denotes	/
T5009	21888-21891	NN	denotes	BKO
T5011	21892-21898	NNS	denotes	lenses
T5012	21899-21903	VBD	denotes	were
T4997	21904-21912	VBN	denotes	observed
T5013	21912-21913	.	denotes	.
T5014	21913-22299	sentence	denotes	In contrast, radial columns of elongated fiber cells of uniform size and shape containing ball and socket interdigitations were observed in the posterior subcapsular region in 5 wk (Figure 4I), 46 wk (Figure 4L) and 72 wk (data not shown) wild type lenses, and fiber cells in contact with the posterior capsule could be traced back to the equatorial bow region and anterior epithelium.
T5015	21914-21916	IN	denotes	In
T5017	21917-21925	NN	denotes	contrast
T5018	21925-21927	,	denotes	,
T5019	21927-21933	JJ	denotes	radial
T5020	21934-21941	NNS	denotes	columns
T5021	21942-21944	IN	denotes	of
T5022	21945-21954	VBN	denotes	elongated
T5024	21955-21960	NN	denotes	fiber
T5023	21961-21966	NNS	denotes	cells
T5025	21967-21969	IN	denotes	of
T5026	21970-21977	JJ	denotes	uniform
T5027	21978-21982	NN	denotes	size
T5028	21983-21986	CC	denotes	and
T5029	21987-21992	NN	denotes	shape
T5030	21993-22003	VBG	denotes	containing
T5031	22004-22008	NN	denotes	ball
T5033	22009-22012	CC	denotes	and
T5034	22013-22019	NN	denotes	socket
T5032	22020-22036	NNS	denotes	interdigitations
T5035	22037-22041	VBD	denotes	were
T5016	22042-22050	VBN	denotes	observed
T5036	22051-22053	IN	denotes	in
T5037	22054-22057	DT	denotes	the
T5039	22058-22067	JJ	denotes	posterior
T5040	22068-22079	JJ	denotes	subcapsular
T5038	22080-22086	NN	denotes	region
T5041	22087-22089	IN	denotes	in
T5042	22090-22091	CD	denotes	5
T5043	22092-22094	NN	denotes	wk
T5045	22095-22096	-LRB-	denotes	(
T5047	22096-22102	NN	denotes	Figure
T5046	22103-22105	NN	denotes	4I
T5048	22105-22106	-RRB-	denotes	)
T5049	22106-22108	,	denotes	,
T5050	22108-22110	CD	denotes	46
T5051	22111-22113	NN	denotes	wk
T5052	22114-22115	-LRB-	denotes	(
T5054	22115-22121	NN	denotes	Figure
T5053	22122-22124	NN	denotes	4L
T5055	22124-22125	-RRB-	denotes	)
T5056	22126-22129	CC	denotes	and
T5057	22130-22132	CD	denotes	72
T5058	22133-22135	NN	denotes	wk
T5059	22136-22137	-LRB-	denotes	(
T5061	22137-22141	NNS	denotes	data
T5062	22142-22145	RB	denotes	not
T5060	22146-22151	VBN	denotes	shown
T5063	22151-22152	-RRB-	denotes	)
T5064	22153-22157	JJ	denotes	wild
T5065	22158-22162	NN	denotes	type
T5044	22163-22169	NNS	denotes	lenses
T5066	22169-22171	,	denotes	,
T5067	22171-22174	CC	denotes	and
T5068	22175-22180	NN	denotes	fiber
T5069	22181-22186	NNS	denotes	cells
T5071	22187-22189	IN	denotes	in
T5072	22190-22197	NN	denotes	contact
T5073	22198-22202	IN	denotes	with
T5074	22203-22206	DT	denotes	the
T5076	22207-22216	JJ	denotes	posterior
T5075	22217-22224	NN	denotes	capsule
T5077	22225-22230	MD	denotes	could
T5078	22231-22233	VB	denotes	be
T5070	22234-22240	VBN	denotes	traced
T5079	22241-22245	RB	denotes	back
T5080	22246-22248	IN	denotes	to
T5081	22249-22252	DT	denotes	the
T5083	22253-22263	JJ	denotes	equatorial
T5084	22264-22267	NN	denotes	bow
T5082	22268-22274	NN	denotes	region
T5085	22275-22278	CC	denotes	and
T5086	22279-22287	JJ	denotes	anterior
T5087	22288-22298	NN	denotes	epithelium
T5088	22298-22299	.	denotes	.
T8758	22310-22324	JJ	denotes	Representative
T8760	22325-22333	NN	denotes	scanning
T8761	22334-22342	NN	denotes	electron
T8759	22343-22354	NNS	denotes	micrographs
T8762	22355-22357	IN	denotes	of
T8763	22358-22364	NN	denotes	alphaA
T8765	22364-22365	HYPH	denotes	/
T8764	22365-22368	NN	denotes	BKO
T8767	22369-22370	-LRB-	denotes	(
T8768	22370-22371	NN	denotes	A
T8769	22371-22372	SYM	denotes	-
T8770	22372-22373	NN	denotes	F
T8771	22373-22374	-RRB-	denotes	)
T8772	22375-22378	CC	denotes	and
T8773	22379-22383	JJ	denotes	wild
T8774	22384-22388	NN	denotes	type
T8775	22389-22390	-LRB-	denotes	(
T8776	22390-22391	NN	denotes	G
T8777	22391-22392	SYM	denotes	-
T8778	22392-22393	NN	denotes	L
T8779	22393-22394	-RRB-	denotes	)
T8766	22395-22401	NNS	denotes	lenses
T8780	22401-22402	.	denotes	.
T8781	22402-22459	sentence	denotes	(A-C) Representative images from 5 wk alphaA/BKO lenses.
T8782	22403-22404	-LRB-	denotes	(
T8783	22404-22405	LS	denotes	A
T8785	22405-22406	SYM	denotes	-
T8786	22406-22407	LS	denotes	C
T8787	22407-22408	-RRB-	denotes	)
T8788	22409-22423	JJ	denotes	Representative
T8784	22424-22430	NNS	denotes	images
T8789	22431-22435	IN	denotes	from
T8790	22436-22437	CD	denotes	5
T8791	22438-22440	NN	denotes	wk
T8793	22441-22447	NN	denotes	alphaA
T8795	22447-22448	HYPH	denotes	/
T8794	22448-22451	NN	denotes	BKO
T8792	22452-22458	NNS	denotes	lenses
T8796	22458-22459	.	denotes	.
T8797	22459-22517	sentence	denotes	(D-F) Representative images from 54 wk alphaA/BKO lenses.
T8798	22460-22461	-LRB-	denotes	(
T8799	22461-22462	LS	denotes	D
T8801	22462-22463	SYM	denotes	-
T8802	22463-22464	LS	denotes	F
T8803	22464-22465	-RRB-	denotes	)
T8804	22466-22480	JJ	denotes	Representative
T8800	22481-22487	NNS	denotes	images
T8805	22488-22492	IN	denotes	from
T8806	22493-22495	CD	denotes	54
T8807	22496-22498	NN	denotes	wk
T8809	22499-22505	NN	denotes	alphaA
T8811	22505-22506	HYPH	denotes	/
T8810	22506-22509	NN	denotes	BKO
T8808	22510-22516	NNS	denotes	lenses
T8812	22516-22517	.	denotes	.
T8813	22517-22573	sentence	denotes	(G-I) Representative images from 5 wk wild type lenses.
T8814	22518-22519	-LRB-	denotes	(
T8815	22519-22520	LS	denotes	G
T8817	22520-22521	SYM	denotes	-
T8818	22521-22522	LS	denotes	I
T8819	22522-22523	-RRB-	denotes	)
T8820	22524-22538	JJ	denotes	Representative
T8816	22539-22545	NNS	denotes	images
T8821	22546-22550	IN	denotes	from
T8822	22551-22552	CD	denotes	5
T8823	22553-22555	NN	denotes	wk
T8825	22556-22560	JJ	denotes	wild
T8826	22561-22565	NN	denotes	type
T8824	22566-22572	NNS	denotes	lenses
T8827	22572-22573	.	denotes	.
T8828	22573-22630	sentence	denotes	(J-L) Representative images from 46 wk wild type lenses.
T8829	22574-22575	-LRB-	denotes	(
T8830	22575-22576	LS	denotes	J
T8832	22576-22577	SYM	denotes	-
T8833	22577-22578	LS	denotes	L
T8834	22578-22579	-RRB-	denotes	)
T8835	22580-22594	JJ	denotes	Representative
T8831	22595-22601	NNS	denotes	images
T8836	22602-22606	IN	denotes	from
T8837	22607-22609	CD	denotes	46
T8838	22610-22612	NN	denotes	wk
T8840	22613-22617	JJ	denotes	wild
T8841	22618-22622	NN	denotes	type
T8839	22623-22629	NNS	denotes	lenses
T8842	22629-22630	.	denotes	.
T8843	22630-22670	sentence	denotes	(A, D, G, and J) Equatorial/bow region.
T8844	22631-22632	-LRB-	denotes	(
T8845	22632-22633	LS	denotes	A
T8847	22633-22635	,	denotes	,
T8848	22635-22636	LS	denotes	D
T8849	22636-22638	,	denotes	,
T8850	22638-22639	LS	denotes	G
T8851	22639-22641	,	denotes	,
T8852	22641-22644	CC	denotes	and
T8853	22645-22646	LS	denotes	J
T8854	22646-22647	-RRB-	denotes	)
T8855	22648-22658	JJ	denotes	Equatorial
T8857	22658-22659	HYPH	denotes	/
T8856	22659-22662	JJ	denotes	bow
T8846	22663-22669	NN	denotes	region
T8858	22669-22670	.	denotes	.
T8859	22670-22704	sentence	denotes	(B, E, H, and K) Anterior cortex.
T8860	22671-22672	-LRB-	denotes	(
T8861	22672-22673	LS	denotes	B
T8863	22673-22675	,	denotes	,
T8864	22675-22676	LS	denotes	E
T8865	22676-22678	,	denotes	,
T8866	22678-22679	LS	denotes	H
T8867	22679-22681	,	denotes	,
T8868	22681-22684	CC	denotes	and
T8869	22685-22686	LS	denotes	K
T8870	22686-22687	-RRB-	denotes	)
T8871	22688-22696	JJ	denotes	Anterior
T8862	22697-22703	NN	denotes	cortex
T8872	22703-22704	.	denotes	.
T8873	22704-22751	sentence	denotes	C, (F, I, and L) Posterior subcapsular region.
T8874	22705-22706	LS	denotes	C
T8876	22706-22708	,	denotes	,
T8877	22708-22709	-LRB-	denotes	(
T8878	22709-22710	LS	denotes	F
T8879	22710-22712	,	denotes	,
T8880	22712-22713	LS	denotes	I
T8881	22713-22715	,	denotes	,
T8882	22715-22718	CC	denotes	and
T8883	22719-22720	LS	denotes	L
T8884	22720-22721	-RRB-	denotes	)
T8885	22722-22731	JJ	denotes	Posterior
T8886	22732-22743	JJ	denotes	subcapsular
T8875	22744-22750	NN	denotes	region
T8887	22750-22751	.	denotes	.
T5961	22764-22767	CD	denotes	One
T5962	22768-22776	NN	denotes	approach
T5964	22777-22779	IN	denotes	to
T5965	22780-22789	VBG	denotes	resolving
T5966	22790-22794	DT	denotes	some
T5967	22795-22797	IN	denotes	of
T5968	22798-22801	DT	denotes	the
T5970	22802-22804	FW	denotes	in
T5971	22805-22809	FW	denotes	vivo
T5969	22810-22819	NNS	denotes	functions
T5972	22820-22822	IN	denotes	of
T5973	22823-22828	NN	denotes	alpha
T5975	22828-22829	HYPH	denotes	-
T5974	22829-22839	NN	denotes	crystallin
T5963	22840-22842	VBZ	denotes	is
T5976	22843-22845	TO	denotes	to
T5977	22846-22854	VB	denotes	generate
T5978	22855-22861	NN	denotes	animal
T5979	22862-22868	NNS	denotes	models
T5980	22869-22874	WRB	denotes	where
T5982	22875-22878	CD	denotes	one
T5983	22879-22881	CC	denotes	or
T5984	22882-22886	DT	denotes	both
T5985	22887-22889	IN	denotes	of
T5986	22890-22893	DT	denotes	the
T5988	22894-22899	NN	denotes	alpha
T5990	22899-22900	HYPH	denotes	-
T5989	22900-22910	NN	denotes	crystallin
T5991	22911-22915	NN	denotes	gene
T5987	22916-22924	NNS	denotes	products
T5992	22925-22929	VBP	denotes	have
T5993	22930-22934	VBN	denotes	been
T5981	22935-22945	VBN	denotes	eliminated
T5994	22945-22946	.	denotes	.
T5995	22946-23188	sentence	denotes	Brady et al. [22] demonstrated, by targeted disruption of the mouse alphaA gene, that this protein was essential for the maintenance of lens transparency, possibly by maintaining the solubility of alphaB, or associated proteins, in the lens.
T5996	22947-22952	NNP	denotes	Brady
T5998	22953-22955	FW	denotes	et
T5999	22956-22959	FW	denotes	al.
T6000	22960-22961	-LRB-	denotes	[
T6001	22961-22963	CD	denotes	22
T6002	22963-22964	-RRB-	denotes	]
T5997	22965-22977	VBD	denotes	demonstrated
T6003	22977-22979	,	denotes	,
T6004	22979-22981	IN	denotes	by
T6005	22982-22990	VBN	denotes	targeted
T6006	22991-23001	NN	denotes	disruption
T6007	23002-23004	IN	denotes	of
T6008	23005-23008	DT	denotes	the
T6010	23009-23014	NN	denotes	mouse
T6011	23015-23021	NN	denotes	alphaA
T6009	23022-23026	NN	denotes	gene
T6012	23026-23028	,	denotes	,
T6013	23028-23032	IN	denotes	that
T6015	23033-23037	DT	denotes	this
T6016	23038-23045	NN	denotes	protein
T6014	23046-23049	VBD	denotes	was
T6017	23050-23059	JJ	denotes	essential
T6018	23060-23063	IN	denotes	for
T6019	23064-23067	DT	denotes	the
T6020	23068-23079	NN	denotes	maintenance
T6021	23080-23082	IN	denotes	of
T6022	23083-23087	NN	denotes	lens
T6023	23088-23100	NN	denotes	transparency
T6024	23100-23102	,	denotes	,
T6025	23102-23110	RB	denotes	possibly
T6026	23111-23113	IN	denotes	by
T6027	23114-23125	VBG	denotes	maintaining
T6028	23126-23129	DT	denotes	the
T6029	23130-23140	NN	denotes	solubility
T6030	23141-23143	IN	denotes	of
T6031	23144-23150	NN	denotes	alphaB
T6032	23150-23152	,	denotes	,
T6033	23152-23154	CC	denotes	or
T6034	23155-23165	VBN	denotes	associated
T6035	23166-23174	NN	denotes	proteins
T6036	23174-23176	,	denotes	,
T6037	23176-23178	IN	denotes	in
T6038	23179-23182	DT	denotes	the
T6039	23183-23187	NN	denotes	lens
T6040	23187-23188	.	denotes	.
T6041	23188-23383	sentence	denotes	These lenses were also reported to be smaller in equatorial and axial dimensions than age matched wild type lens, which was very similar to that which was observed with the double knockout lens.
T6042	23189-23194	DT	denotes	These
T6043	23195-23201	NNS	denotes	lenses
T6045	23202-23206	VBD	denotes	were
T6046	23207-23211	RB	denotes	also
T6044	23212-23220	VBN	denotes	reported
T6047	23221-23223	TO	denotes	to
T6048	23224-23226	VB	denotes	be
T6049	23227-23234	JJR	denotes	smaller
T6050	23235-23237	IN	denotes	in
T6051	23238-23248	JJ	denotes	equatorial
T6053	23249-23252	CC	denotes	and
T6054	23253-23258	JJ	denotes	axial
T6052	23259-23269	NNS	denotes	dimensions
T6055	23270-23274	IN	denotes	than
T6056	23275-23278	NN	denotes	age
T6057	23279-23286	VBN	denotes	matched
T6059	23287-23291	JJ	denotes	wild
T6060	23292-23296	NN	denotes	type
T6058	23297-23301	NN	denotes	lens
T6061	23301-23303	,	denotes	,
T6062	23303-23308	WDT	denotes	which
T6063	23309-23312	VBD	denotes	was
T6064	23313-23317	RB	denotes	very
T6065	23318-23325	JJ	denotes	similar
T6066	23326-23328	IN	denotes	to
T6067	23329-23333	DT	denotes	that
T6068	23334-23339	WDT	denotes	which
T6070	23340-23343	VBD	denotes	was
T6069	23344-23352	VBN	denotes	observed
T6071	23353-23357	IN	denotes	with
T6072	23358-23361	DT	denotes	the
T6074	23362-23368	NN	denotes	double
T6075	23369-23377	NN	denotes	knockout
T6073	23378-23382	NN	denotes	lens
T6076	23382-23383	.	denotes	.
T6077	23383-23537	sentence	denotes	Targeted disruption of the mouse alphaB gene, however, resulted in lenses similar in size to aged-matched wild type lens with no cataracts reported [23].
T6078	23384-23392	VBN	denotes	Targeted
T6079	23393-23403	NN	denotes	disruption
T6081	23404-23406	IN	denotes	of
T6082	23407-23410	DT	denotes	the
T6084	23411-23416	NN	denotes	mouse
T6085	23417-23423	NN	denotes	alphaB
T6083	23424-23428	NN	denotes	gene
T6086	23428-23430	,	denotes	,
T6087	23430-23437	RB	denotes	however
T6088	23437-23439	,	denotes	,
T6080	23439-23447	VBD	denotes	resulted
T6089	23448-23450	IN	denotes	in
T6090	23451-23457	NNS	denotes	lenses
T6091	23458-23465	JJ	denotes	similar
T6092	23466-23468	IN	denotes	in
T6093	23469-23473	NN	denotes	size
T6094	23474-23476	IN	denotes	to
T6095	23477-23481	JJ	denotes	aged
T6097	23481-23482	HYPH	denotes	-
T6096	23482-23489	JJ	denotes	matched
T6099	23490-23494	JJ	denotes	wild
T6100	23495-23499	NN	denotes	type
T6098	23500-23504	NN	denotes	lens
T6101	23505-23509	IN	denotes	with
T6102	23510-23512	DT	denotes	no
T6103	23513-23522	NNS	denotes	cataracts
T6104	23523-23531	VBN	denotes	reported
T6105	23532-23533	-LRB-	denotes	[
T6106	23533-23535	CD	denotes	23
T6107	23535-23536	-RRB-	denotes	]
T6108	23536-23537	.	denotes	.
T6109	23537-23645	sentence	denotes	This indicates that alphaA may play a greater role in maintaining the transparency of the lens then alphaB.
T6110	23538-23542	DT	denotes	This
T6111	23543-23552	VBZ	denotes	indicates
T6112	23553-23557	IN	denotes	that
T6114	23558-23564	NN	denotes	alphaA
T6115	23565-23568	MD	denotes	may
T6113	23569-23573	VB	denotes	play
T6116	23574-23575	DT	denotes	a
T6118	23576-23583	JJR	denotes	greater
T6117	23584-23588	NN	denotes	role
T6119	23589-23591	IN	denotes	in
T6120	23592-23603	VBG	denotes	maintaining
T6121	23604-23607	DT	denotes	the
T6122	23608-23620	NN	denotes	transparency
T6123	23621-23623	IN	denotes	of
T6124	23624-23627	DT	denotes	the
T6125	23628-23632	NN	denotes	lens
T6126	23633-23637	IN	denotes	then
T6127	23638-23644	NN	denotes	alphaB
T6128	23644-23645	.	denotes	.
T6129	23645-23898	sentence	denotes	In the single alpha-crystallin knockout mice, the remaining alpha-crystallin may fully or partially compensate for some of the functions of the missing protein, especially in the lens, where both alphaA and alphaB are normally expressed at high levels.
T6130	23646-23648	IN	denotes	In
T6132	23649-23652	DT	denotes	the
T6134	23653-23659	JJ	denotes	single
T6136	23660-23665	NN	denotes	alpha
T6138	23665-23666	HYPH	denotes	-
T6137	23666-23676	NN	denotes	crystallin
T6135	23677-23685	NN	denotes	knockout
T6133	23686-23690	NNS	denotes	mice
T6139	23690-23692	,	denotes	,
T6140	23692-23695	DT	denotes	the
T6142	23696-23705	VBG	denotes	remaining
T6143	23706-23711	NN	denotes	alpha
T6144	23711-23712	HYPH	denotes	-
T6141	23712-23722	NN	denotes	crystallin
T6145	23723-23726	MD	denotes	may
T6146	23727-23732	RB	denotes	fully
T6147	23733-23735	CC	denotes	or
T6148	23736-23745	RB	denotes	partially
T6131	23746-23756	VB	denotes	compensate
T6149	23757-23760	IN	denotes	for
T6150	23761-23765	DT	denotes	some
T6151	23766-23768	IN	denotes	of
T6152	23769-23772	DT	denotes	the
T6153	23773-23782	NNS	denotes	functions
T6154	23783-23785	IN	denotes	of
T6155	23786-23789	DT	denotes	the
T6157	23790-23797	VBG	denotes	missing
T6156	23798-23805	NN	denotes	protein
T6158	23805-23807	,	denotes	,
T6159	23807-23817	RB	denotes	especially
T6160	23818-23820	IN	denotes	in
T6161	23821-23824	DT	denotes	the
T6162	23825-23829	NN	denotes	lens
T6163	23829-23831	,	denotes	,
T6164	23831-23836	WRB	denotes	where
T6166	23837-23841	CC	denotes	both
T6167	23842-23848	NN	denotes	alphaA
T6168	23849-23852	CC	denotes	and
T6169	23853-23859	NN	denotes	alphaB
T6170	23860-23863	VBP	denotes	are
T6171	23864-23872	RB	denotes	normally
T6165	23873-23882	VBN	denotes	expressed
T6172	23883-23885	IN	denotes	at
T6173	23886-23890	JJ	denotes	high
T6174	23891-23897	NNS	denotes	levels
T6175	23897-23898	.	denotes	.
T6176	23898-24320	sentence	denotes	This was supported by the morphological observation made in this study of no posterior sutures or fiber cells extending to the posterior capsule of the lens, ectopically staining nucleic acids in the posterior subcapsular region of 5 wk and anterior subcapsular cortex of 54 wk, gross morphological differences in the equatorial/bow, posterior and anterior regions of lenses from alphaA/BKO mice as compared to wild mice.
T6177	23899-23903	DT	denotes	This
T6179	23904-23907	VBD	denotes	was
T6178	23908-23917	VBN	denotes	supported
T6180	23918-23920	IN	denotes	by
T6181	23921-23924	DT	denotes	the
T6183	23925-23938	JJ	denotes	morphological
T6182	23939-23950	NN	denotes	observation
T6184	23951-23955	VBN	denotes	made
T6185	23956-23958	IN	denotes	in
T6186	23959-23963	DT	denotes	this
T6187	23964-23969	NN	denotes	study
T6188	23970-23972	IN	denotes	of
T6189	23973-23975	DT	denotes	no
T6191	23976-23985	JJ	denotes	posterior
T6190	23986-23993	NNS	denotes	sutures
T6192	23994-23996	CC	denotes	or
T6193	23997-24002	NN	denotes	fiber
T6194	24003-24008	NNS	denotes	cells
T6195	24009-24018	VBG	denotes	extending
T6196	24019-24021	IN	denotes	to
T6197	24022-24025	DT	denotes	the
T6199	24026-24035	JJ	denotes	posterior
T6198	24036-24043	NN	denotes	capsule
T6200	24044-24046	IN	denotes	of
T6201	24047-24050	DT	denotes	the
T6202	24051-24055	NN	denotes	lens
T6203	24055-24057	,	denotes	,
T6204	24057-24068	RB	denotes	ectopically
T6205	24069-24077	VBG	denotes	staining
T6207	24078-24085	JJ	denotes	nucleic
T6206	24086-24091	NNS	denotes	acids
T6208	24092-24094	IN	denotes	in
T6209	24095-24098	DT	denotes	the
T6211	24099-24108	JJ	denotes	posterior
T6212	24109-24120	JJ	denotes	subcapsular
T6210	24121-24127	NN	denotes	region
T6213	24128-24130	IN	denotes	of
T6214	24131-24132	CD	denotes	5
T6215	24133-24135	NN	denotes	wk
T6216	24136-24139	CC	denotes	and
T6217	24140-24148	JJ	denotes	anterior
T6219	24149-24160	JJ	denotes	subcapsular
T6218	24161-24167	NN	denotes	cortex
T6220	24168-24170	IN	denotes	of
T6221	24171-24173	CD	denotes	54
T6222	24174-24176	NN	denotes	wk
T6223	24176-24178	,	denotes	,
T6224	24178-24183	JJ	denotes	gross
T6226	24184-24197	JJ	denotes	morphological
T6225	24198-24209	NNS	denotes	differences
T6227	24210-24212	IN	denotes	in
T6228	24213-24216	DT	denotes	the
T6230	24217-24227	JJ	denotes	equatorial
T6232	24227-24228	HYPH	denotes	/
T6231	24228-24231	JJ	denotes	bow
T6233	24231-24233	,	denotes	,
T6234	24233-24242	JJ	denotes	posterior
T6235	24243-24246	CC	denotes	and
T6236	24247-24255	JJ	denotes	anterior
T6229	24256-24263	NNS	denotes	regions
T6237	24264-24266	IN	denotes	of
T6238	24267-24273	NNS	denotes	lenses
T6239	24274-24278	IN	denotes	from
T6240	24279-24285	NN	denotes	alphaA
T6242	24285-24286	HYPH	denotes	/
T6241	24286-24289	NN	denotes	BKO
T6243	24290-24294	NNS	denotes	mice
T6244	24295-24297	IN	denotes	as
T6245	24298-24306	VBN	denotes	compared
T6246	24307-24309	IN	denotes	to
T6247	24310-24314	JJ	denotes	wild
T6248	24315-24319	NNS	denotes	mice
T6249	24319-24320	.	denotes	.
T6250	24320-24425	sentence	denotes	None of these morphological differences have been reported in the single alphaA or alphaB knockout mice.
T6251	24321-24325	NN	denotes	None
T6253	24326-24328	IN	denotes	of
T6254	24329-24334	DT	denotes	these
T6256	24335-24348	JJ	denotes	morphological
T6255	24349-24360	NNS	denotes	differences
T6257	24361-24365	VBP	denotes	have
T6258	24366-24370	VBN	denotes	been
T6252	24371-24379	VBN	denotes	reported
T6259	24380-24382	IN	denotes	in
T6260	24383-24386	DT	denotes	the
T6262	24387-24393	JJ	denotes	single
T6264	24394-24400	NN	denotes	alphaA
T6265	24401-24403	CC	denotes	or
T6266	24404-24410	NN	denotes	alphaB
T6263	24411-24419	NN	denotes	knockout
T6261	24420-24424	NNS	denotes	mice
T6267	24424-24425	.	denotes	.
T6268	24425-24618	sentence	denotes	It must be noted, however, that the alphaA/BKO mice also lack the HSPB2 gene product [23] and the contribution of this protein to normal lens morphology and functions should not be overlooked.
T6269	24426-24428	PRP	denotes	It
T6271	24429-24433	MD	denotes	must
T6272	24434-24436	VB	denotes	be
T6270	24437-24442	VBN	denotes	noted
T6273	24442-24444	,	denotes	,
T6274	24444-24451	RB	denotes	however
T6275	24451-24453	,	denotes	,
T6276	24453-24457	IN	denotes	that
T6278	24458-24461	DT	denotes	the
T6280	24462-24468	NN	denotes	alphaA
T6282	24468-24469	HYPH	denotes	/
T6281	24469-24472	NN	denotes	BKO
T6279	24473-24477	NNS	denotes	mice
T6283	24478-24482	RB	denotes	also
T6277	24483-24487	VBP	denotes	lack
T6284	24488-24491	DT	denotes	the
T6286	24492-24497	NN	denotes	HSPB2
T6287	24498-24502	NN	denotes	gene
T6285	24503-24510	NN	denotes	product
T6288	24511-24512	-LRB-	denotes	[
T6289	24512-24514	CD	denotes	23
T6290	24514-24515	-RRB-	denotes	]
T6291	24516-24519	CC	denotes	and
T6292	24520-24523	DT	denotes	the
T6293	24524-24536	NN	denotes	contribution
T6295	24537-24539	IN	denotes	of
T6296	24540-24544	DT	denotes	this
T6297	24545-24552	NN	denotes	protein
T6298	24553-24555	IN	denotes	to
T6299	24556-24562	JJ	denotes	normal
T6301	24563-24567	NN	denotes	lens
T6300	24568-24578	NN	denotes	morphology
T6302	24579-24582	CC	denotes	and
T6303	24583-24592	NNS	denotes	functions
T6304	24593-24599	MD	denotes	should
T6305	24600-24603	RB	denotes	not
T6306	24604-24606	VB	denotes	be
T6294	24607-24617	VBN	denotes	overlooked
T6307	24617-24618	.	denotes	.
T6308	24618-24696	sentence	denotes	Future studies should address the possible functions of HSPB2 in normal lens.
T6309	24619-24625	JJ	denotes	Future
T6310	24626-24633	NNS	denotes	studies
T6312	24634-24640	MD	denotes	should
T6311	24641-24648	VB	denotes	address
T6313	24649-24652	DT	denotes	the
T6315	24653-24661	JJ	denotes	possible
T6314	24662-24671	NNS	denotes	functions
T6316	24672-24674	IN	denotes	of
T6317	24675-24680	NN	denotes	HSPB2
T6318	24681-24683	IN	denotes	in
T6319	24684-24690	JJ	denotes	normal
T6320	24691-24695	NN	denotes	lens
T6321	24695-24696	.	denotes	.
T6322	24696-24850	sentence	denotes	The results of the current study support the hypothesis that alpha-crystallin plays an active role in the differentiation and growth of lens fiber cells.
T6323	24697-24700	DT	denotes	The
T6324	24701-24708	NNS	denotes	results
T6326	24709-24711	IN	denotes	of
T6327	24712-24715	DT	denotes	the
T6329	24716-24723	JJ	denotes	current
T6328	24724-24729	NN	denotes	study
T6325	24730-24737	VBP	denotes	support
T6330	24738-24741	DT	denotes	the
T6331	24742-24752	NN	denotes	hypothesis
T6332	24753-24757	IN	denotes	that
T6334	24758-24763	NN	denotes	alpha
T6336	24763-24764	HYPH	denotes	-
T6335	24764-24774	NN	denotes	crystallin
T6333	24775-24780	VBZ	denotes	plays
T6337	24781-24783	DT	denotes	an
T6339	24784-24790	JJ	denotes	active
T6338	24791-24795	NN	denotes	role
T6340	24796-24798	IN	denotes	in
T6341	24799-24802	DT	denotes	the
T6342	24803-24818	NN	denotes	differentiation
T6343	24819-24822	CC	denotes	and
T6344	24823-24829	NN	denotes	growth
T6345	24830-24832	IN	denotes	of
T6346	24833-24837	NN	denotes	lens
T6347	24838-24843	NN	denotes	fiber
T6348	24844-24849	NNS	denotes	cells
T6349	24849-24850	.	denotes	.
T6350	24850-25103	sentence	denotes	Normal differentiation of lens fiber cells consists of a progression from a simple cuboidal epithelial cell, containing a nucleus and a minimal numbers of organelles, to a stratified layer of elongated fiber-like cells, devoid of nuclei and organelles.
T6351	24851-24857	JJ	denotes	Normal
T6352	24858-24873	NN	denotes	differentiation
T6354	24874-24876	IN	denotes	of
T6355	24877-24881	NN	denotes	lens
T6356	24882-24887	NN	denotes	fiber
T6357	24888-24893	NNS	denotes	cells
T6353	24894-24902	VBZ	denotes	consists
T6358	24903-24905	IN	denotes	of
T6359	24906-24907	DT	denotes	a
T6360	24908-24919	NN	denotes	progression
T6361	24920-24924	IN	denotes	from
T6362	24925-24926	DT	denotes	a
T6364	24927-24933	JJ	denotes	simple
T6365	24934-24942	JJ	denotes	cuboidal
T6366	24943-24953	JJ	denotes	epithelial
T6363	24954-24958	NN	denotes	cell
T6367	24958-24960	,	denotes	,
T6368	24960-24970	VBG	denotes	containing
T6369	24971-24972	DT	denotes	a
T6370	24973-24980	NN	denotes	nucleus
T6371	24981-24984	CC	denotes	and
T6372	24985-24986	DT	denotes	a
T6374	24987-24994	JJ	denotes	minimal
T6373	24995-25002	NNS	denotes	numbers
T6375	25003-25005	IN	denotes	of
T6376	25006-25016	NNS	denotes	organelles
T6377	25016-25018	,	denotes	,
T6378	25018-25020	IN	denotes	to
T6379	25021-25022	DT	denotes	a
T6381	25023-25033	JJ	denotes	stratified
T6380	25034-25039	NN	denotes	layer
T6382	25040-25042	IN	denotes	of
T6383	25043-25052	VBN	denotes	elongated
T6385	25053-25058	NN	denotes	fiber
T6387	25058-25059	HYPH	denotes	-
T6386	25059-25063	JJ	denotes	like
T6384	25064-25069	NNS	denotes	cells
T6388	25069-25071	,	denotes	,
T6389	25071-25077	JJ	denotes	devoid
T6390	25078-25080	IN	denotes	of
T6391	25081-25087	NNS	denotes	nuclei
T6392	25088-25091	CC	denotes	and
T6393	25092-25102	NNS	denotes	organelles
T6394	25102-25103	.	denotes	.
T6395	25103-25392	sentence	denotes	Differentiation of epithelial cells occurs in the equatorial/bow region of the lens, where epithelial cells begin to elongate and differentiate into fiber cells of uniform cellular shape, arranged in radial columns of cells extending from the anterior epithelium to the posterior capsule.
T6396	25104-25119	NN	denotes	Differentiation
T6398	25120-25122	IN	denotes	of
T6399	25123-25133	JJ	denotes	epithelial
T6400	25134-25139	NNS	denotes	cells
T6397	25140-25146	VBZ	denotes	occurs
T6401	25147-25149	IN	denotes	in
T6402	25150-25153	DT	denotes	the
T6404	25154-25164	JJ	denotes	equatorial
T6406	25164-25165	HYPH	denotes	/
T6405	25165-25168	JJ	denotes	bow
T6403	25169-25175	NN	denotes	region
T6407	25176-25178	IN	denotes	of
T6408	25179-25182	DT	denotes	the
T6409	25183-25187	NN	denotes	lens
T6410	25187-25189	,	denotes	,
T6411	25189-25194	WRB	denotes	where
T6413	25195-25205	JJ	denotes	epithelial
T6414	25206-25211	NNS	denotes	cells
T6412	25212-25217	VBP	denotes	begin
T6415	25218-25220	TO	denotes	to
T6416	25221-25229	VB	denotes	elongate
T6417	25230-25233	CC	denotes	and
T6418	25234-25247	VB	denotes	differentiate
T6419	25248-25252	IN	denotes	into
T6420	25253-25258	NN	denotes	fiber
T6421	25259-25264	NNS	denotes	cells
T6422	25265-25267	IN	denotes	of
T6423	25268-25275	JJ	denotes	uniform
T6425	25276-25284	JJ	denotes	cellular
T6424	25285-25290	NN	denotes	shape
T6426	25290-25292	,	denotes	,
T6427	25292-25300	VBN	denotes	arranged
T6428	25301-25303	IN	denotes	in
T6429	25304-25310	JJ	denotes	radial
T6430	25311-25318	NNS	denotes	columns
T6431	25319-25321	IN	denotes	of
T6432	25322-25327	NNS	denotes	cells
T6433	25328-25337	VBG	denotes	extending
T6434	25338-25342	IN	denotes	from
T6435	25343-25346	DT	denotes	the
T6437	25347-25355	JJ	denotes	anterior
T6436	25356-25366	NN	denotes	epithelium
T6438	25367-25369	IN	denotes	to
T6439	25370-25373	DT	denotes	the
T6441	25374-25383	JJ	denotes	posterior
T6440	25384-25391	NN	denotes	capsule
T6442	25391-25392	.	denotes	.
T6443	25392-25484	sentence	denotes	This process did not appear to have proceeded normally in lenses lacking alphaA and alphaB.
T6444	25393-25397	DT	denotes	This
T6445	25398-25405	NN	denotes	process
T6447	25406-25409	VBD	denotes	did
T6448	25410-25413	RB	denotes	not
T6446	25414-25420	VB	denotes	appear
T6449	25421-25423	TO	denotes	to
T6451	25424-25428	VB	denotes	have
T6450	25429-25438	VBN	denotes	proceeded
T6452	25439-25447	RB	denotes	normally
T6453	25448-25450	IN	denotes	in
T6454	25451-25457	NNS	denotes	lenses
T6455	25458-25465	VBG	denotes	lacking
T6456	25466-25472	NN	denotes	alphaA
T6457	25473-25476	CC	denotes	and
T6458	25477-25483	NN	denotes	alphaB
T6459	25483-25484	.	denotes	.
T6460	25484-25801	sentence	denotes	The morphological observations presented in this study demonstrate that fiber cells in lenses lacking alphaA and alphaB fail to elongate symmetrically from the bow region and therefore do not establish the typical "onion skin" conformation in which cells extend from the anterior epithelium to the posterior capsule.
T6461	25485-25488	DT	denotes	The
T6463	25489-25502	JJ	denotes	morphological
T6462	25503-25515	NNS	denotes	observations
T6465	25516-25525	VBN	denotes	presented
T6466	25526-25528	IN	denotes	in
T6467	25529-25533	DT	denotes	this
T6468	25534-25539	NN	denotes	study
T6464	25540-25551	VBP	denotes	demonstrate
T6469	25552-25556	IN	denotes	that
T6471	25557-25562	NN	denotes	fiber
T6472	25563-25568	NNS	denotes	cells
T6473	25569-25571	IN	denotes	in
T6474	25572-25578	NNS	denotes	lenses
T6475	25579-25586	VBG	denotes	lacking
T6476	25587-25593	NN	denotes	alphaA
T6477	25594-25597	CC	denotes	and
T6478	25598-25604	NN	denotes	alphaB
T6470	25605-25609	VBP	denotes	fail
T6479	25610-25612	TO	denotes	to
T6480	25613-25621	VB	denotes	elongate
T6481	25622-25635	RB	denotes	symmetrically
T6482	25636-25640	IN	denotes	from
T6483	25641-25644	DT	denotes	the
T6485	25645-25648	NN	denotes	bow
T6484	25649-25655	NN	denotes	region
T6486	25656-25659	CC	denotes	and
T6487	25660-25669	RB	denotes	therefore
T6489	25670-25672	VBP	denotes	do
T6490	25673-25676	RB	denotes	not
T6488	25677-25686	VB	denotes	establish
T6491	25687-25690	DT	denotes	the
T6493	25691-25698	JJ	denotes	typical
T6494	25699-25700	``	denotes	"
T6495	25700-25705	NN	denotes	onion
T6496	25706-25710	NN	denotes	skin
T6497	25710-25711	''	denotes	"
T6492	25712-25724	NN	denotes	conformation
T6498	25725-25727	IN	denotes	in
T6500	25728-25733	WDT	denotes	which
T6501	25734-25739	NNS	denotes	cells
T6499	25740-25746	VBP	denotes	extend
T6502	25747-25751	IN	denotes	from
T6503	25752-25755	DT	denotes	the
T6505	25756-25764	JJ	denotes	anterior
T6504	25765-25775	NN	denotes	epithelium
T6506	25776-25778	IN	denotes	to
T6507	25779-25782	DT	denotes	the
T6509	25783-25792	JJ	denotes	posterior
T6508	25793-25800	NN	denotes	capsule
T6510	25800-25801	.	denotes	.
T6511	25801-26008	sentence	denotes	Additionally, in lenses from 54 wk alphaA/BKO lenses, there was a persistence of cell nuclei in deeper cortical regions, and ectopic cell nuclei were present in large numbers in the anterior central cortex.
T6512	25802-25814	RB	denotes	Additionally
T6514	25814-25816	,	denotes	,
T6515	25816-25818	IN	denotes	in
T6516	25819-25825	NNS	denotes	lenses
T6517	25826-25830	IN	denotes	from
T6518	25831-25833	CD	denotes	54
T6519	25834-25836	NN	denotes	wk
T6521	25837-25843	NN	denotes	alphaA
T6523	25843-25844	HYPH	denotes	/
T6522	25844-25847	NN	denotes	BKO
T6520	25848-25854	NNS	denotes	lenses
T6524	25854-25856	,	denotes	,
T6525	25856-25861	EX	denotes	there
T6513	25862-25865	VBD	denotes	was
T6526	25866-25867	DT	denotes	a
T6527	25868-25879	NN	denotes	persistence
T6528	25880-25882	IN	denotes	of
T6529	25883-25887	NN	denotes	cell
T6530	25888-25894	NNS	denotes	nuclei
T6531	25895-25897	IN	denotes	in
T6532	25898-25904	JJR	denotes	deeper
T6534	25905-25913	JJ	denotes	cortical
T6533	25914-25921	NNS	denotes	regions
T6535	25921-25923	,	denotes	,
T6536	25923-25926	CC	denotes	and
T6537	25927-25934	JJ	denotes	ectopic
T6539	25935-25939	NN	denotes	cell
T6538	25940-25946	NNS	denotes	nuclei
T6540	25947-25951	VBD	denotes	were
T6541	25952-25959	JJ	denotes	present
T6542	25960-25962	IN	denotes	in
T6543	25963-25968	JJ	denotes	large
T6544	25969-25976	NNS	denotes	numbers
T6545	25977-25979	IN	denotes	in
T6546	25980-25983	DT	denotes	the
T6548	25984-25992	JJ	denotes	anterior
T6549	25993-26000	JJ	denotes	central
T6547	26001-26007	NN	denotes	cortex
T6550	26007-26008	.	denotes	.
T6551	26008-26085	sentence	denotes	At 5 wks of age cell nuclei were present, adjacent to the posterior capsule.
T6552	26009-26011	IN	denotes	At
T6554	26012-26013	CD	denotes	5
T6555	26014-26017	NNS	denotes	wks
T6556	26018-26020	IN	denotes	of
T6557	26021-26024	NN	denotes	age
T6558	26025-26029	NN	denotes	cell
T6559	26030-26036	NNS	denotes	nuclei
T6553	26037-26041	VBD	denotes	were
T6560	26042-26049	JJ	denotes	present
T6561	26049-26051	,	denotes	,
T6562	26051-26059	JJ	denotes	adjacent
T6563	26060-26062	IN	denotes	to
T6564	26063-26066	DT	denotes	the
T6566	26067-26076	JJ	denotes	posterior
T6565	26077-26084	NN	denotes	capsule
T6567	26084-26085	.	denotes	.
T6568	26085-26228	sentence	denotes	These morphological observations are consistent with a defect in the normal differentiation pathway of lens epithelial cells into fiber cells.
T6569	26086-26091	DT	denotes	These
T6571	26092-26105	JJ	denotes	morphological
T6570	26106-26118	NNS	denotes	observations
T6572	26119-26122	VBP	denotes	are
T6573	26123-26133	JJ	denotes	consistent
T6574	26134-26138	IN	denotes	with
T6575	26139-26140	DT	denotes	a
T6576	26141-26147	NN	denotes	defect
T6577	26148-26150	IN	denotes	in
T6578	26151-26154	DT	denotes	the
T6580	26155-26161	JJ	denotes	normal
T6581	26162-26177	NN	denotes	differentiation
T6579	26178-26185	NN	denotes	pathway
T6582	26186-26188	IN	denotes	of
T6583	26189-26193	NN	denotes	lens
T6585	26194-26204	JJ	denotes	epithelial
T6584	26205-26210	NNS	denotes	cells
T6586	26211-26215	IN	denotes	into
T6587	26216-26221	NN	denotes	fiber
T6588	26222-26227	NNS	denotes	cells
T6589	26227-26228	.	denotes	.
T6590	26228-26471	sentence	denotes	It is unlikely that these alterations in alphaA/BKO mouse lenses result from increased susceptibility of these lenses to light-induced damage in the absence of the molecular chaperone protection afforded by alphaA and alphaB in normal lenses.
T6591	26229-26231	PRP	denotes	It
T6592	26232-26234	VBZ	denotes	is
T6593	26235-26243	JJ	denotes	unlikely
T6594	26244-26248	IN	denotes	that
T6596	26249-26254	DT	denotes	these
T6597	26255-26266	NNS	denotes	alterations
T6598	26267-26269	IN	denotes	in
T6599	26270-26276	NN	denotes	alphaA
T6601	26276-26277	HYPH	denotes	/
T6600	26277-26280	NN	denotes	BKO
T6603	26281-26286	NN	denotes	mouse
T6602	26287-26293	NNS	denotes	lenses
T6595	26294-26300	VBP	denotes	result
T6604	26301-26305	IN	denotes	from
T6605	26306-26315	VBN	denotes	increased
T6606	26316-26330	NN	denotes	susceptibility
T6607	26331-26333	IN	denotes	of
T6608	26334-26339	DT	denotes	these
T6609	26340-26346	NNS	denotes	lenses
T6610	26347-26349	IN	denotes	to
T6611	26350-26355	NN	denotes	light
T6613	26355-26356	HYPH	denotes	-
T6612	26356-26363	VBN	denotes	induced
T6614	26364-26370	NN	denotes	damage
T6615	26371-26373	IN	denotes	in
T6616	26374-26377	DT	denotes	the
T6617	26378-26385	NN	denotes	absence
T6618	26386-26388	IN	denotes	of
T6619	26389-26392	DT	denotes	the
T6621	26393-26402	JJ	denotes	molecular
T6622	26403-26412	NN	denotes	chaperone
T6620	26413-26423	NN	denotes	protection
T6623	26424-26432	VBN	denotes	afforded
T6624	26433-26435	IN	denotes	by
T6625	26436-26442	NN	denotes	alphaA
T6626	26443-26446	CC	denotes	and
T6627	26447-26453	NN	denotes	alphaB
T6628	26454-26456	IN	denotes	in
T6629	26457-26463	JJ	denotes	normal
T6630	26464-26470	NNS	denotes	lenses
T6631	26470-26471	.	denotes	.
T6632	26471-26667	sentence	denotes	With the normal time of eye opening at approximately 14 days after birth, the 5 wk old mice had their eyes open and lenses exposed to light for only about 3 weeks prior to morphological analysis.
T6633	26472-26476	IN	denotes	With
T6635	26477-26480	DT	denotes	the
T6637	26481-26487	JJ	denotes	normal
T6636	26488-26492	NN	denotes	time
T6638	26493-26495	IN	denotes	of
T6639	26496-26499	NN	denotes	eye
T6640	26500-26507	NN	denotes	opening
T6641	26508-26510	IN	denotes	at
T6642	26511-26524	RB	denotes	approximately
T6643	26525-26527	CD	denotes	14
T6644	26528-26532	NNS	denotes	days
T6645	26533-26538	IN	denotes	after
T6646	26539-26544	NN	denotes	birth
T6647	26544-26546	,	denotes	,
T6648	26546-26549	DT	denotes	the
T6650	26550-26551	CD	denotes	5
T6651	26552-26554	NN	denotes	wk
T6652	26555-26558	JJ	denotes	old
T6649	26559-26563	NNS	denotes	mice
T6634	26564-26567	VBD	denotes	had
T6653	26568-26573	PRP$	denotes	their
T6654	26574-26578	NNS	denotes	eyes
T6655	26579-26583	JJ	denotes	open
T6656	26584-26587	CC	denotes	and
T6657	26588-26594	NNS	denotes	lenses
T6658	26595-26602	VBN	denotes	exposed
T6659	26603-26605	IN	denotes	to
T6660	26606-26611	NN	denotes	light
T6661	26612-26615	IN	denotes	for
T6662	26616-26620	RB	denotes	only
T6664	26621-26626	RB	denotes	about
T6663	26627-26628	CD	denotes	3
T6665	26629-26634	NNS	denotes	weeks
T6666	26635-26640	RB	denotes	prior
T6667	26641-26643	IN	denotes	to
T6668	26644-26657	JJ	denotes	morphological
T6669	26658-26666	NN	denotes	analysis
T6670	26666-26667	.	denotes	.
T6671	26667-26804	sentence	denotes	Moreover, these animals had been exposed to only animal facility fluorescent lighting and were protected from UV light by plastic cages.
T6672	26668-26676	RB	denotes	Moreover
T6674	26676-26678	,	denotes	,
T6675	26678-26683	DT	denotes	these
T6676	26684-26691	NNS	denotes	animals
T6677	26692-26695	VBD	denotes	had
T6678	26696-26700	VBN	denotes	been
T6673	26701-26708	VBN	denotes	exposed
T6679	26709-26711	IN	denotes	to
T6680	26712-26716	RB	denotes	only
T6682	26717-26723	JJ	denotes	animal
T6683	26724-26732	NN	denotes	facility
T6684	26733-26744	JJ	denotes	fluorescent
T6681	26745-26753	NN	denotes	lighting
T6685	26754-26757	CC	denotes	and
T6687	26758-26762	VBD	denotes	were
T6686	26763-26772	VBN	denotes	protected
T6688	26773-26777	IN	denotes	from
T6689	26778-26780	NN	denotes	UV
T6690	26781-26786	NN	denotes	light
T6691	26787-26789	IN	denotes	by
T6692	26790-26797	JJ	denotes	plastic
T6693	26798-26803	NNS	denotes	cages
T6694	26803-26804	.	denotes	.
T6695	26804-27101	sentence	denotes	If lack of protection from light-induced damage was the major factor affecting the changes in these lenses, then the bulk of the damage should have resided along the visual axis, particularly in the central anterior epithelium and subcapsular cortex in the 5 wk lenses, but this was not the case.
T6696	26805-26807	IN	denotes	If
T6698	26808-26812	NN	denotes	lack
T6699	26813-26815	IN	denotes	of
T6700	26816-26826	NN	denotes	protection
T6701	26827-26831	IN	denotes	from
T6702	26832-26837	NN	denotes	light
T6704	26837-26838	HYPH	denotes	-
T6703	26838-26845	VBN	denotes	induced
T6705	26846-26852	NN	denotes	damage
T6697	26853-26856	VBD	denotes	was
T6707	26857-26860	DT	denotes	the
T6709	26861-26866	JJ	denotes	major
T6708	26867-26873	NN	denotes	factor
T6710	26874-26883	VBG	denotes	affecting
T6711	26884-26887	DT	denotes	the
T6712	26888-26895	NNS	denotes	changes
T6713	26896-26898	IN	denotes	in
T6714	26899-26904	DT	denotes	these
T6715	26905-26911	NNS	denotes	lenses
T6716	26911-26913	,	denotes	,
T6717	26913-26917	RB	denotes	then
T6718	26918-26921	DT	denotes	the
T6719	26922-26926	NN	denotes	bulk
T6720	26927-26929	IN	denotes	of
T6721	26930-26933	DT	denotes	the
T6722	26934-26940	NN	denotes	damage
T6723	26941-26947	MD	denotes	should
T6724	26948-26952	VB	denotes	have
T6706	26953-26960	VBN	denotes	resided
T6725	26961-26966	IN	denotes	along
T6726	26967-26970	DT	denotes	the
T6728	26971-26977	JJ	denotes	visual
T6727	26978-26982	NN	denotes	axis
T6729	26982-26984	,	denotes	,
T6730	26984-26996	RB	denotes	particularly
T6731	26997-26999	IN	denotes	in
T6732	27000-27003	DT	denotes	the
T6734	27004-27011	JJ	denotes	central
T6735	27012-27020	JJ	denotes	anterior
T6733	27021-27031	NN	denotes	epithelium
T6736	27032-27035	CC	denotes	and
T6737	27036-27047	JJ	denotes	subcapsular
T6738	27048-27054	NN	denotes	cortex
T6739	27055-27057	IN	denotes	in
T6740	27058-27061	DT	denotes	the
T6742	27062-27063	CD	denotes	5
T6743	27064-27066	NN	denotes	wk
T6741	27067-27073	NNS	denotes	lenses
T6744	27073-27075	,	denotes	,
T6745	27075-27078	CC	denotes	but
T6746	27079-27083	DT	denotes	this
T6747	27084-27087	VBD	denotes	was
T6748	27088-27091	RB	denotes	not
T6749	27092-27095	DT	denotes	the
T6750	27096-27100	NN	denotes	case
T6751	27100-27101	.	denotes	.
T6752	27101-27213	sentence	denotes	In these lenses, gross morphological changes were apparent in the equatorial and posterior subcapsular regions.
T6753	27102-27104	IN	denotes	In
T6755	27105-27110	DT	denotes	these
T6756	27111-27117	NNS	denotes	lenses
T6757	27117-27119	,	denotes	,
T6758	27119-27124	JJ	denotes	gross
T6760	27125-27138	JJ	denotes	morphological
T6759	27139-27146	NNS	denotes	changes
T6754	27147-27151	VBD	denotes	were
T6761	27152-27160	JJ	denotes	apparent
T6762	27161-27163	IN	denotes	in
T6763	27164-27167	DT	denotes	the
T6765	27168-27178	JJ	denotes	equatorial
T6766	27179-27182	CC	denotes	and
T6767	27183-27192	JJ	denotes	posterior
T6768	27193-27204	JJ	denotes	subcapsular
T6764	27205-27212	NNS	denotes	regions
T6769	27212-27213	.	denotes	.
T6770	27213-27418	sentence	denotes	These changes included posterior subcapsular nucleic acid staining, absence of posterior sutures, and small irregularly shaped cells, not arranged in any discernable pattern, in the equatorial/bow region.
T6771	27214-27219	DT	denotes	These
T6772	27220-27227	NNS	denotes	changes
T6773	27228-27236	VBD	denotes	included
T6774	27237-27246	JJ	denotes	posterior
T6776	27247-27258	JJ	denotes	subcapsular
T6777	27259-27266	JJ	denotes	nucleic
T6778	27267-27271	NN	denotes	acid
T6775	27272-27280	NN	denotes	staining
T6779	27280-27282	,	denotes	,
T6780	27282-27289	NN	denotes	absence
T6781	27290-27292	IN	denotes	of
T6782	27293-27302	JJ	denotes	posterior
T6783	27303-27310	NNS	denotes	sutures
T6784	27310-27312	,	denotes	,
T6785	27312-27315	CC	denotes	and
T6786	27316-27321	JJ	denotes	small
T6788	27322-27333	RB	denotes	irregularly
T6789	27334-27340	VBN	denotes	shaped
T6787	27341-27346	NNS	denotes	cells
T6790	27346-27348	,	denotes	,
T6791	27348-27351	RB	denotes	not
T6792	27352-27360	VBN	denotes	arranged
T6793	27361-27363	IN	denotes	in
T6794	27364-27367	DT	denotes	any
T6796	27368-27379	JJ	denotes	discernable
T6795	27380-27387	NN	denotes	pattern
T6797	27387-27389	,	denotes	,
T6798	27389-27391	IN	denotes	in
T6799	27392-27395	DT	denotes	the
T6801	27396-27406	JJ	denotes	equatorial
T6803	27406-27407	HYPH	denotes	/
T6802	27407-27410	JJ	denotes	bow
T6800	27411-27417	NN	denotes	region
T6804	27417-27418	.	denotes	.
T6805	27418-27630	sentence	denotes	Systemic stress factors crossing the blood/aqueous barrier might explain some morphological changes at the equatorial region, but this would not explain nucleic acid staining in the posterior subcapsular region.
T6806	27419-27427	JJ	denotes	Systemic
T6807	27428-27434	NN	denotes	stress
T6808	27435-27442	NNS	denotes	factors
T6810	27443-27451	VBG	denotes	crossing
T6811	27452-27455	DT	denotes	the
T6813	27456-27461	NN	denotes	blood
T6815	27461-27462	HYPH	denotes	/
T6814	27462-27469	JJ	denotes	aqueous
T6812	27470-27477	NN	denotes	barrier
T6816	27478-27483	MD	denotes	might
T6809	27484-27491	VB	denotes	explain
T6817	27492-27496	DT	denotes	some
T6819	27497-27510	JJ	denotes	morphological
T6818	27511-27518	NNS	denotes	changes
T6820	27519-27521	IN	denotes	at
T6821	27522-27525	DT	denotes	the
T6823	27526-27536	JJ	denotes	equatorial
T6822	27537-27543	NN	denotes	region
T6824	27543-27545	,	denotes	,
T6825	27545-27548	CC	denotes	but
T6826	27549-27553	DT	denotes	this
T6828	27554-27559	MD	denotes	would
T6829	27560-27563	RB	denotes	not
T6827	27564-27571	VB	denotes	explain
T6830	27572-27579	JJ	denotes	nucleic
T6831	27580-27584	NN	denotes	acid
T6832	27585-27593	NN	denotes	staining
T6833	27594-27596	IN	denotes	in
T6834	27597-27600	DT	denotes	the
T6836	27601-27610	JJ	denotes	posterior
T6837	27611-27622	JJ	denotes	subcapsular
T6835	27623-27629	NN	denotes	region
T6838	27629-27630	.	denotes	.
T6839	27630-27886	sentence	denotes	In the 5 wk alphaA/BKO lenses, nucleic acid staining in the posterior subcapsular region is consistent with either anterior epithelial cells migrating aberrantly to the posterior pole, or primary fiber cells failing to fully differentiate by 5 wks of age.
T6840	27631-27633	IN	denotes	In
T6842	27634-27637	DT	denotes	the
T6844	27638-27639	CD	denotes	5
T6845	27640-27642	NN	denotes	wk
T6846	27643-27649	NN	denotes	alphaA
T6848	27649-27650	HYPH	denotes	/
T6847	27650-27653	NN	denotes	BKO
T6843	27654-27660	NNS	denotes	lenses
T6849	27660-27662	,	denotes	,
T6850	27662-27669	JJ	denotes	nucleic
T6851	27670-27674	NN	denotes	acid
T6852	27675-27683	NN	denotes	staining
T6853	27684-27686	IN	denotes	in
T6854	27687-27690	DT	denotes	the
T6856	27691-27700	JJ	denotes	posterior
T6857	27701-27712	JJ	denotes	subcapsular
T6855	27713-27719	NN	denotes	region
T6841	27720-27722	VBZ	denotes	is
T6858	27723-27733	JJ	denotes	consistent
T6859	27734-27738	IN	denotes	with
T6860	27739-27745	CC	denotes	either
T6862	27746-27754	JJ	denotes	anterior
T6863	27755-27765	JJ	denotes	epithelial
T6861	27766-27771	NNS	denotes	cells
T6864	27772-27781	VBG	denotes	migrating
T6865	27782-27792	RB	denotes	aberrantly
T6866	27793-27795	IN	denotes	to
T6867	27796-27799	DT	denotes	the
T6869	27800-27809	JJ	denotes	posterior
T6868	27810-27814	NN	denotes	pole
T6870	27814-27816	,	denotes	,
T6871	27816-27818	CC	denotes	or
T6872	27819-27826	JJ	denotes	primary
T6874	27827-27832	NN	denotes	fiber
T6873	27833-27838	NNS	denotes	cells
T6875	27839-27846	VBG	denotes	failing
T6876	27847-27849	TO	denotes	to
T6878	27850-27855	RB	denotes	fully
T6877	27856-27869	VB	denotes	differentiate
T6879	27870-27872	IN	denotes	by
T6880	27873-27874	CD	denotes	5
T6881	27875-27878	NNS	denotes	wks
T6882	27879-27881	IN	denotes	of
T6883	27882-27885	NN	denotes	age
T6884	27885-27886	.	denotes	.
T6885	27886-28016	sentence	denotes	These two possibilities could not be differentiated in the methods employed, and were beyond the objectives of the current study.
T6886	27887-27892	DT	denotes	These
T6888	27893-27896	CD	denotes	two
T6887	27897-27910	NNS	denotes	possibilities
T6890	27911-27916	MD	denotes	could
T6891	27917-27920	RB	denotes	not
T6892	27921-27923	VB	denotes	be
T6889	27924-27938	VBN	denotes	differentiated
T6893	27939-27941	IN	denotes	in
T6894	27942-27945	DT	denotes	the
T6895	27946-27953	NNS	denotes	methods
T6896	27954-27962	VBN	denotes	employed
T6897	27962-27964	,	denotes	,
T6898	27964-27967	CC	denotes	and
T6899	27968-27972	VBD	denotes	were
T6900	27973-27979	IN	denotes	beyond
T6901	27980-27983	DT	denotes	the
T6902	27984-27994	NNS	denotes	objectives
T6903	27995-27997	IN	denotes	of
T6904	27998-28001	DT	denotes	the
T6906	28002-28009	JJ	denotes	current
T6905	28010-28015	NN	denotes	study
T6907	28015-28016	.	denotes	.
T6908	28016-28163	sentence	denotes	Future studies are being designed to address which of these two processes might explain nucleic acid staining in the posterior subcapsular region.
T6909	28017-28023	JJ	denotes	Future
T6910	28024-28031	NNS	denotes	studies
T6912	28032-28035	VBP	denotes	are
T6913	28036-28041	VBG	denotes	being
T6911	28042-28050	VBN	denotes	designed
T6914	28051-28053	TO	denotes	to
T6915	28054-28061	VB	denotes	address
T6916	28062-28067	WDT	denotes	which
T6918	28068-28070	IN	denotes	of
T6919	28071-28076	DT	denotes	these
T6921	28077-28080	CD	denotes	two
T6920	28081-28090	NNS	denotes	processes
T6922	28091-28096	MD	denotes	might
T6917	28097-28104	VB	denotes	explain
T6923	28105-28112	JJ	denotes	nucleic
T6924	28113-28117	NN	denotes	acid
T6925	28118-28126	NN	denotes	staining
T6926	28127-28129	IN	denotes	in
T6927	28130-28133	DT	denotes	the
T6929	28134-28143	JJ	denotes	posterior
T6930	28144-28155	JJ	denotes	subcapsular
T6928	28156-28162	NN	denotes	region
T6931	28162-28163	.	denotes	.
T6932	28163-28276	sentence	denotes	Staining in this region was not observed in older alphaA/BKO lenses, suggesting that this pattern was transient.
T6933	28164-28172	VBG	denotes	Staining
T6935	28173-28175	IN	denotes	in
T6936	28176-28180	DT	denotes	this
T6937	28181-28187	NN	denotes	region
T6938	28188-28191	VBD	denotes	was
T6939	28192-28195	RB	denotes	not
T6934	28196-28204	VBN	denotes	observed
T6940	28205-28207	IN	denotes	in
T6941	28208-28213	JJR	denotes	older
T6943	28214-28220	NN	denotes	alphaA
T6945	28220-28221	HYPH	denotes	/
T6944	28221-28224	NN	denotes	BKO
T6942	28225-28231	NNS	denotes	lenses
T6946	28231-28233	,	denotes	,
T6947	28233-28243	VBG	denotes	suggesting
T6948	28244-28248	IN	denotes	that
T6950	28249-28253	DT	denotes	this
T6951	28254-28261	NN	denotes	pattern
T6949	28262-28265	VBD	denotes	was
T6952	28266-28275	JJ	denotes	transient
T6953	28275-28276	.	denotes	.
T6954	28276-28447	sentence	denotes	The fate of the nucleic acid-containing cells in the posterior capsular region of younger lenses is not known at this time, nor is the morphology of earlier stage lenses.
T6955	28277-28280	DT	denotes	The
T6956	28281-28285	NN	denotes	fate
T6958	28286-28288	IN	denotes	of
T6959	28289-28292	DT	denotes	the
T6961	28293-28300	JJ	denotes	nucleic
T6962	28301-28305	NN	denotes	acid
T6964	28305-28306	HYPH	denotes	-
T6963	28306-28316	VBG	denotes	containing
T6960	28317-28322	NNS	denotes	cells
T6965	28323-28325	IN	denotes	in
T6966	28326-28329	DT	denotes	the
T6968	28330-28339	JJ	denotes	posterior
T6969	28340-28348	JJ	denotes	capsular
T6967	28349-28355	NN	denotes	region
T6970	28356-28358	IN	denotes	of
T6971	28359-28366	JJR	denotes	younger
T6972	28367-28373	NNS	denotes	lenses
T6973	28374-28376	VBZ	denotes	is
T6974	28377-28380	RB	denotes	not
T6957	28381-28386	VBN	denotes	known
T6975	28387-28389	IN	denotes	at
T6976	28390-28394	DT	denotes	this
T6977	28395-28399	NN	denotes	time
T6978	28399-28401	,	denotes	,
T6979	28401-28404	CC	denotes	nor
T6980	28405-28407	VBZ	denotes	is
T6981	28408-28411	DT	denotes	the
T6982	28412-28422	NN	denotes	morphology
T6983	28423-28425	IN	denotes	of
T6984	28426-28433	JJR	denotes	earlier
T6985	28434-28439	NN	denotes	stage
T6986	28440-28446	NNS	denotes	lenses
T6987	28446-28447	.	denotes	.
T6988	28447-28593	sentence	denotes	Future studies with defined objectives to address the development and progression of morphological changes seen in this study are being designed.
T6989	28448-28454	JJ	denotes	Future
T6990	28455-28462	NNS	denotes	studies
T6992	28463-28467	IN	denotes	with
T6993	28468-28475	VBN	denotes	defined
T6994	28476-28486	NNS	denotes	objectives
T6995	28487-28489	TO	denotes	to
T6996	28490-28497	VB	denotes	address
T6997	28498-28501	DT	denotes	the
T6998	28502-28513	NN	denotes	development
T6999	28514-28517	CC	denotes	and
T7000	28518-28529	NN	denotes	progression
T7001	28530-28532	IN	denotes	of
T7002	28533-28546	JJ	denotes	morphological
T7003	28547-28554	NNS	denotes	changes
T7004	28555-28559	VBN	denotes	seen
T7005	28560-28562	IN	denotes	in
T7006	28563-28567	DT	denotes	this
T7007	28568-28573	NN	denotes	study
T7008	28574-28577	VBP	denotes	are
T7009	28578-28583	VBG	denotes	being
T6991	28584-28592	VBN	denotes	designed
T7010	28592-28593	.	denotes	.
T7011	28593-28821	sentence	denotes	The morphological differences in alphaA/BKO lenses, compared to age matched wild type lenses, were consistent with the hypothesis that alpha-crystallin plays an active role in the differentiation and growth of lens fiber cells.
T7012	28594-28597	DT	denotes	The
T7014	28598-28611	JJ	denotes	morphological
T7013	28612-28623	NNS	denotes	differences
T7016	28624-28626	IN	denotes	in
T7017	28627-28633	NN	denotes	alphaA
T7019	28633-28634	HYPH	denotes	/
T7018	28634-28637	NN	denotes	BKO
T7020	28638-28644	NNS	denotes	lenses
T7021	28644-28646	,	denotes	,
T7022	28646-28654	VBN	denotes	compared
T7023	28655-28657	IN	denotes	to
T7024	28658-28661	NN	denotes	age
T7025	28662-28669	VBN	denotes	matched
T7027	28670-28674	JJ	denotes	wild
T7028	28675-28679	NN	denotes	type
T7026	28680-28686	NNS	denotes	lenses
T7029	28686-28688	,	denotes	,
T7015	28688-28692	VBD	denotes	were
T7030	28693-28703	JJ	denotes	consistent
T7031	28704-28708	IN	denotes	with
T7032	28709-28712	DT	denotes	the
T7033	28713-28723	NN	denotes	hypothesis
T7034	28724-28728	IN	denotes	that
T7036	28729-28734	NN	denotes	alpha
T7038	28734-28735	HYPH	denotes	-
T7037	28735-28745	NN	denotes	crystallin
T7035	28746-28751	VBZ	denotes	plays
T7039	28752-28754	DT	denotes	an
T7041	28755-28761	JJ	denotes	active
T7040	28762-28766	NN	denotes	role
T7042	28767-28769	IN	denotes	in
T7043	28770-28773	DT	denotes	the
T7044	28774-28789	NN	denotes	differentiation
T7045	28790-28793	CC	denotes	and
T7046	28794-28800	NN	denotes	growth
T7047	28801-28803	IN	denotes	of
T7048	28804-28808	NN	denotes	lens
T7049	28809-28814	NN	denotes	fiber
T7050	28815-28820	NNS	denotes	cells
T7051	28820-28821	.	denotes	.
T7052	28821-28915	sentence	denotes	In addition, it was clearly evident that alpha-crystallin is necessary for lens transparency.
T7053	28822-28824	IN	denotes	In
T7055	28825-28833	NN	denotes	addition
T7056	28833-28835	,	denotes	,
T7057	28835-28837	PRP	denotes	it
T7054	28838-28841	VBD	denotes	was
T7058	28842-28849	RB	denotes	clearly
T7059	28850-28857	JJ	denotes	evident
T7060	28858-28862	IN	denotes	that
T7062	28863-28868	NN	denotes	alpha
T7064	28868-28869	HYPH	denotes	-
T7063	28869-28879	NN	denotes	crystallin
T7061	28880-28882	VBZ	denotes	is
T7065	28883-28892	JJ	denotes	necessary
T7066	28893-28896	IN	denotes	for
T7067	28897-28901	NN	denotes	lens
T7068	28902-28914	NN	denotes	transparency
T7069	28914-28915	.	denotes	.
T7070	28915-29095	sentence	denotes	The final biological event in a lens epithelial cell's life is the transformation from an epithelial cell into a fiber cell, which occurs at the equatorial/bow region of the lens.
T7071	28916-28919	DT	denotes	The
T7073	28920-28925	JJ	denotes	final
T7074	28926-28936	JJ	denotes	biological
T7072	28937-28942	NN	denotes	event
T7076	28943-28945	IN	denotes	in
T7077	28946-28947	DT	denotes	a
T7079	28948-28952	NN	denotes	lens
T7080	28953-28963	JJ	denotes	epithelial
T7078	28964-28968	NN	denotes	cell
T7082	28968-28970	POS	denotes	's
T7081	28971-28975	NN	denotes	life
T7075	28976-28978	VBZ	denotes	is
T7083	28979-28982	DT	denotes	the
T7084	28983-28997	NN	denotes	transformation
T7085	28998-29002	IN	denotes	from
T7086	29003-29005	DT	denotes	an
T7088	29006-29016	JJ	denotes	epithelial
T7087	29017-29021	NN	denotes	cell
T7089	29022-29026	IN	denotes	into
T7090	29027-29028	DT	denotes	a
T7092	29029-29034	NN	denotes	fiber
T7091	29035-29039	NN	denotes	cell
T7093	29039-29041	,	denotes	,
T7094	29041-29046	WDT	denotes	which
T7095	29047-29053	VBZ	denotes	occurs
T7096	29054-29056	IN	denotes	at
T7097	29057-29060	DT	denotes	the
T7099	29061-29071	JJ	denotes	equatorial
T7101	29071-29072	HYPH	denotes	/
T7100	29072-29075	JJ	denotes	bow
T7098	29076-29082	NN	denotes	region
T7102	29083-29085	IN	denotes	of
T7103	29086-29089	DT	denotes	the
T7104	29090-29094	NN	denotes	lens
T7105	29094-29095	.	denotes	.
T7106	29095-29269	sentence	denotes	The newly forming fiber cell continues to differentiate in the cortex until a mature fiber cell devoid of organelles with suture formation at the ends of the cell is formed.
T7107	29096-29099	DT	denotes	The
T7109	29100-29105	RB	denotes	newly
T7110	29106-29113	VBG	denotes	forming
T7111	29114-29119	NN	denotes	fiber
T7108	29120-29124	NN	denotes	cell
T7112	29125-29134	VBZ	denotes	continues
T7113	29135-29137	TO	denotes	to
T7114	29138-29151	VB	denotes	differentiate
T7115	29152-29154	IN	denotes	in
T7116	29155-29158	DT	denotes	the
T7117	29159-29165	NN	denotes	cortex
T7118	29166-29171	IN	denotes	until
T7120	29172-29173	DT	denotes	a
T7122	29174-29180	JJ	denotes	mature
T7123	29181-29186	NN	denotes	fiber
T7121	29187-29191	NN	denotes	cell
T7124	29192-29198	JJ	denotes	devoid
T7125	29199-29201	IN	denotes	of
T7126	29202-29212	NNS	denotes	organelles
T7127	29213-29217	IN	denotes	with
T7128	29218-29224	NN	denotes	suture
T7129	29225-29234	NN	denotes	formation
T7130	29235-29237	IN	denotes	at
T7131	29238-29241	DT	denotes	the
T7132	29242-29246	NNS	denotes	ends
T7133	29247-29249	IN	denotes	of
T7134	29250-29253	DT	denotes	the
T7135	29254-29258	NN	denotes	cell
T7136	29259-29261	VBZ	denotes	is
T7119	29262-29268	VBN	denotes	formed
T7137	29268-29269	.	denotes	.
T7138	29269-29367	sentence	denotes	This entire process from epithelial cell to mature fiber cell is defined as lens differentiation.
T7139	29270-29274	DT	denotes	This
T7141	29275-29281	JJ	denotes	entire
T7140	29282-29289	NN	denotes	process
T7143	29290-29294	IN	denotes	from
T7144	29295-29305	JJ	denotes	epithelial
T7145	29306-29310	NN	denotes	cell
T7146	29311-29313	IN	denotes	to
T7147	29314-29320	JJ	denotes	mature
T7149	29321-29326	NN	denotes	fiber
T7148	29327-29331	NN	denotes	cell
T7150	29332-29334	VBZ	denotes	is
T7142	29335-29342	VBN	denotes	defined
T7151	29343-29345	IN	denotes	as
T7152	29346-29350	NN	denotes	lens
T7153	29351-29366	NN	denotes	differentiation
T7154	29366-29367	.	denotes	.
T7155	29367-29622	sentence	denotes	The precise spatial and temporal expression of the crystallin proteins in the developing lens may not be simply a consequence of the differentiation process, but instead may play an important, if not essential, role in the differentiation process itself.
T7156	29368-29371	DT	denotes	The
T7158	29372-29379	JJ	denotes	precise
T7159	29380-29387	JJ	denotes	spatial
T7160	29388-29391	CC	denotes	and
T7161	29392-29400	JJ	denotes	temporal
T7157	29401-29411	NN	denotes	expression
T7163	29412-29414	IN	denotes	of
T7164	29415-29418	DT	denotes	the
T7166	29419-29429	NN	denotes	crystallin
T7165	29430-29438	NN	denotes	proteins
T7167	29439-29441	IN	denotes	in
T7168	29442-29445	DT	denotes	the
T7170	29446-29456	VBG	denotes	developing
T7169	29457-29461	NN	denotes	lens
T7171	29462-29465	MD	denotes	may
T7172	29466-29469	RB	denotes	not
T7162	29470-29472	VB	denotes	be
T7173	29473-29479	RB	denotes	simply
T7174	29480-29481	DT	denotes	a
T7175	29482-29493	NN	denotes	consequence
T7176	29494-29496	IN	denotes	of
T7177	29497-29500	DT	denotes	the
T7179	29501-29516	NN	denotes	differentiation
T7178	29517-29524	NN	denotes	process
T7180	29524-29526	,	denotes	,
T7181	29526-29529	CC	denotes	but
T7182	29530-29537	RB	denotes	instead
T7184	29538-29541	MD	denotes	may
T7183	29542-29546	VB	denotes	play
T7185	29547-29549	DT	denotes	an
T7187	29550-29559	JJ	denotes	important
T7188	29559-29561	,	denotes	,
T7189	29561-29563	IN	denotes	if
T7190	29564-29567	RB	denotes	not
T7191	29568-29577	JJ	denotes	essential
T7192	29577-29579	,	denotes	,
T7186	29579-29583	NN	denotes	role
T7193	29584-29586	IN	denotes	in
T7194	29587-29590	DT	denotes	the
T7196	29591-29606	NN	denotes	differentiation
T7195	29607-29614	NN	denotes	process
T7197	29615-29621	PRP	denotes	itself
T7198	29621-29622	.	denotes	.
T7199	29622-29680	sentence	denotes	The results of the current study support this hypothesis.
T7200	29623-29626	DT	denotes	The
T7201	29627-29634	NNS	denotes	results
T7203	29635-29637	IN	denotes	of
T7204	29638-29641	DT	denotes	the
T7206	29642-29649	JJ	denotes	current
T7205	29650-29655	NN	denotes	study
T7202	29656-29663	VBP	denotes	support
T7207	29664-29668	DT	denotes	this
T7208	29669-29679	NN	denotes	hypothesis
T7209	29679-29680	.	denotes	.
T7210	29680-29863	sentence	denotes	The exact in vivo molecular mechanisms, by which alpha-crystallin might influence lens epithelial cell differentiation, and maintenance of lens transparency, remain to be determined.
T7211	29681-29684	DT	denotes	The
T7213	29685-29690	JJ	denotes	exact
T7214	29691-29693	FW	denotes	in
T7215	29694-29698	FW	denotes	vivo
T7216	29699-29708	JJ	denotes	molecular
T7212	29709-29719	NNS	denotes	mechanisms
T7218	29719-29721	,	denotes	,
T7219	29721-29723	IN	denotes	by
T7221	29724-29729	WDT	denotes	which
T7222	29730-29735	NN	denotes	alpha
T7224	29735-29736	HYPH	denotes	-
T7223	29736-29746	NN	denotes	crystallin
T7225	29747-29752	MD	denotes	might
T7220	29753-29762	VB	denotes	influence
T7226	29763-29767	NN	denotes	lens
T7228	29768-29778	JJ	denotes	epithelial
T7227	29779-29783	NN	denotes	cell
T7229	29784-29799	NN	denotes	differentiation
T7230	29799-29801	,	denotes	,
T7231	29801-29804	CC	denotes	and
T7232	29805-29816	NN	denotes	maintenance
T7233	29817-29819	IN	denotes	of
T7234	29820-29824	NN	denotes	lens
T7235	29825-29837	NN	denotes	transparency
T7236	29837-29839	,	denotes	,
T7217	29839-29845	VBP	denotes	remain
T7237	29846-29848	TO	denotes	to
T7239	29849-29851	VB	denotes	be
T7238	29852-29862	VBN	denotes	determined
T7240	29862-29863	.	denotes	.
T7241	29863-29917	sentence	denotes	AlphaA and alphaB are members of the shsp family [7].
T7242	29864-29870	NN	denotes	AlphaA
T7244	29871-29874	CC	denotes	and
T7245	29875-29881	NN	denotes	alphaB
T7243	29882-29885	VBP	denotes	are
T7246	29886-29893	NNS	denotes	members
T7247	29894-29896	IN	denotes	of
T7248	29897-29900	DT	denotes	the
T7250	29901-29905	NN	denotes	shsp
T7249	29906-29912	NN	denotes	family
T7251	29913-29914	-LRB-	denotes	[
T7252	29914-29915	CD	denotes	7
T7253	29915-29916	-RRB-	denotes	]
T7254	29916-29917	.	denotes	.
T7255	29917-30129	sentence	denotes	Previous studies have shown that the alpha-crystallin possesses molecular chaperone activity, binding to partially denatured proteins, both in vitro [5] and probably in vivo [6], to inhibit further denaturation.
T7256	29918-29926	JJ	denotes	Previous
T7257	29927-29934	NNS	denotes	studies
T7259	29935-29939	VBP	denotes	have
T7258	29940-29945	VBN	denotes	shown
T7260	29946-29950	IN	denotes	that
T7262	29951-29954	DT	denotes	the
T7264	29955-29960	NN	denotes	alpha
T7265	29960-29961	HYPH	denotes	-
T7263	29961-29971	NN	denotes	crystallin
T7261	29972-29981	VBZ	denotes	possesses
T7266	29982-29991	JJ	denotes	molecular
T7268	29992-30001	NN	denotes	chaperone
T7267	30002-30010	NN	denotes	activity
T7269	30010-30012	,	denotes	,
T7270	30012-30019	VBG	denotes	binding
T7271	30020-30022	IN	denotes	to
T7272	30023-30032	RB	denotes	partially
T7273	30033-30042	VBN	denotes	denatured
T7274	30043-30051	NN	denotes	proteins
T7275	30051-30053	,	denotes	,
T7276	30053-30057	CC	denotes	both
T7278	30058-30060	FW	denotes	in
T7279	30061-30066	FW	denotes	vitro
T7280	30067-30068	-LRB-	denotes	[
T7277	30068-30069	CD	denotes	5
T7281	30069-30070	-RRB-	denotes	]
T7282	30071-30074	CC	denotes	and
T7283	30075-30083	RB	denotes	probably
T7284	30084-30086	FW	denotes	in
T7286	30087-30091	FW	denotes	vivo
T7287	30092-30093	-LRB-	denotes	[
T7285	30093-30094	CD	denotes	6
T7288	30094-30095	-RRB-	denotes	]
T7289	30095-30097	,	denotes	,
T7290	30097-30099	TO	denotes	to
T7291	30100-30107	VB	denotes	inhibit
T7292	30108-30115	JJ	denotes	further
T7293	30116-30128	NN	denotes	denaturation
T7294	30128-30129	.	denotes	.
T7295	30129-30322	sentence	denotes	Although this property may be a major contributor to the maintenance of lens clarity, the early changes in the alphaA/BKO lenses indicate a much broader cellular function for alpha-crystallin.
T7296	30130-30138	IN	denotes	Although
T7298	30139-30143	DT	denotes	this
T7299	30144-30152	NN	denotes	property
T7300	30153-30156	MD	denotes	may
T7297	30157-30159	VB	denotes	be
T7302	30160-30161	DT	denotes	a
T7304	30162-30167	JJ	denotes	major
T7303	30168-30179	NN	denotes	contributor
T7305	30180-30182	IN	denotes	to
T7306	30183-30186	DT	denotes	the
T7307	30187-30198	NN	denotes	maintenance
T7308	30199-30201	IN	denotes	of
T7309	30202-30206	NN	denotes	lens
T7310	30207-30214	NN	denotes	clarity
T7311	30214-30216	,	denotes	,
T7312	30216-30219	DT	denotes	the
T7314	30220-30225	JJ	denotes	early
T7313	30226-30233	NNS	denotes	changes
T7315	30234-30236	IN	denotes	in
T7316	30237-30240	DT	denotes	the
T7318	30241-30247	NN	denotes	alphaA
T7320	30247-30248	HYPH	denotes	/
T7319	30248-30251	NN	denotes	BKO
T7317	30252-30258	NNS	denotes	lenses
T7301	30259-30267	VBP	denotes	indicate
T7321	30268-30269	DT	denotes	a
T7323	30270-30274	RB	denotes	much
T7324	30275-30282	JJR	denotes	broader
T7325	30283-30291	JJ	denotes	cellular
T7322	30292-30300	NN	denotes	function
T7326	30301-30304	IN	denotes	for
T7327	30305-30310	NN	denotes	alpha
T7329	30310-30311	HYPH	denotes	-
T7328	30311-30321	NN	denotes	crystallin
T7330	30321-30322	.	denotes	.
T7331	30322-30437	sentence	denotes	Stress proteins have been shown to be expressed in non-stressed cells during development and differentiation [25].
T7332	30323-30329	NN	denotes	Stress
T7333	30330-30338	NN	denotes	proteins
T7335	30339-30343	VBP	denotes	have
T7336	30344-30348	VBN	denotes	been
T7334	30349-30354	VBN	denotes	shown
T7337	30355-30357	TO	denotes	to
T7339	30358-30360	VB	denotes	be
T7338	30361-30370	VBN	denotes	expressed
T7340	30371-30373	IN	denotes	in
T7341	30374-30386	JJ	denotes	non-stressed
T7342	30387-30392	NNS	denotes	cells
T7343	30393-30399	IN	denotes	during
T7344	30400-30411	NN	denotes	development
T7345	30412-30415	CC	denotes	and
T7346	30416-30431	NN	denotes	differentiation
T7347	30432-30433	-LRB-	denotes	[
T7348	30433-30435	CD	denotes	25
T7349	30435-30436	-RRB-	denotes	]
T7350	30436-30437	.	denotes	.
T7351	30437-30559	sentence	denotes	Hsps were shown to be expressed during the differentiation of mammalian osteoblasts and promelocytic leukemia cells [26].
T7352	30438-30442	NNS	denotes	Hsps
T7354	30443-30447	VBD	denotes	were
T7353	30448-30453	VBN	denotes	shown
T7355	30454-30456	TO	denotes	to
T7357	30457-30459	VB	denotes	be
T7356	30460-30469	VBN	denotes	expressed
T7358	30470-30476	IN	denotes	during
T7359	30477-30480	DT	denotes	the
T7360	30481-30496	NN	denotes	differentiation
T7361	30497-30499	IN	denotes	of
T7362	30500-30509	JJ	denotes	mammalian
T7363	30510-30521	NNS	denotes	osteoblasts
T7364	30522-30525	CC	denotes	and
T7365	30526-30538	JJ	denotes	promelocytic
T7367	30539-30547	NN	denotes	leukemia
T7366	30548-30553	NNS	denotes	cells
T7368	30554-30555	-LRB-	denotes	[
T7369	30555-30557	CD	denotes	26
T7370	30557-30558	-RRB-	denotes	]
T7371	30558-30559	.	denotes	.
T7372	30559-30709	sentence	denotes	In addition, hsp expression has been shown to accompany growth arrest in human B lymphocytes [27] and macrophage differentiation of HL 60 cells [28].
T7373	30560-30562	IN	denotes	In
T7375	30563-30571	NN	denotes	addition
T7376	30571-30573	,	denotes	,
T7377	30573-30576	NN	denotes	hsp
T7378	30577-30587	NN	denotes	expression
T7379	30588-30591	VBZ	denotes	has
T7380	30592-30596	VBN	denotes	been
T7374	30597-30602	VBN	denotes	shown
T7381	30603-30605	TO	denotes	to
T7382	30606-30615	VB	denotes	accompany
T7383	30616-30622	NN	denotes	growth
T7384	30623-30629	NN	denotes	arrest
T7385	30630-30632	IN	denotes	in
T7386	30633-30638	JJ	denotes	human
T7388	30639-30640	NN	denotes	B
T7387	30641-30652	NNS	denotes	lymphocytes
T7389	30653-30654	-LRB-	denotes	[
T7390	30654-30656	CD	denotes	27
T7391	30656-30657	-RRB-	denotes	]
T7392	30658-30661	CC	denotes	and
T7393	30662-30672	NN	denotes	macrophage
T7394	30673-30688	NN	denotes	differentiation
T7395	30689-30691	IN	denotes	of
T7396	30692-30694	NN	denotes	HL
T7398	30695-30697	CD	denotes	60
T7397	30698-30703	NNS	denotes	cells
T7399	30704-30705	-LRB-	denotes	[
T7400	30705-30707	CD	denotes	28
T7401	30707-30708	-RRB-	denotes	]
T7402	30708-30709	.	denotes	.
T7403	30709-30869	sentence	denotes	During myogenic differentiation, mRNA for alphaB increases in conjunction with the induction of mRNA for myogenin, the earliest known event in myogenesis [29].
T7404	30710-30716	IN	denotes	During
T7406	30717-30725	JJ	denotes	myogenic
T7407	30726-30741	NN	denotes	differentiation
T7408	30741-30743	,	denotes	,
T7409	30743-30747	NN	denotes	mRNA
T7410	30748-30751	IN	denotes	for
T7411	30752-30758	NN	denotes	alphaB
T7405	30759-30768	VBZ	denotes	increases
T7412	30769-30771	IN	denotes	in
T7413	30772-30783	NN	denotes	conjunction
T7414	30784-30788	IN	denotes	with
T7415	30789-30792	DT	denotes	the
T7416	30793-30802	NN	denotes	induction
T7417	30803-30805	IN	denotes	of
T7418	30806-30810	NN	denotes	mRNA
T7419	30811-30814	IN	denotes	for
T7420	30815-30823	NN	denotes	myogenin
T7421	30823-30825	,	denotes	,
T7422	30825-30828	DT	denotes	the
T7424	30829-30837	JJS	denotes	earliest
T7425	30838-30843	VBN	denotes	known
T7423	30844-30849	NN	denotes	event
T7426	30850-30852	IN	denotes	in
T7427	30853-30863	NN	denotes	myogenesis
T7428	30864-30865	-LRB-	denotes	[
T7429	30865-30867	CD	denotes	29
T7430	30867-30868	-RRB-	denotes	]
T7431	30868-30869	.	denotes	.
T7432	30869-31044	sentence	denotes	The addition of exogenous alpha-crystallin to primary bovine lens epithelium was shown to induce rapid changes in cell shape, leading to the formation of lentoid bodies [21].
T7433	30870-30873	DT	denotes	The
T7434	30874-30882	NN	denotes	addition
T7436	30883-30885	IN	denotes	of
T7437	30886-30895	JJ	denotes	exogenous
T7439	30896-30901	NN	denotes	alpha
T7440	30901-30902	HYPH	denotes	-
T7438	30902-30912	NN	denotes	crystallin
T7441	30913-30915	IN	denotes	to
T7442	30916-30923	JJ	denotes	primary
T7444	30924-30930	JJ	denotes	bovine
T7445	30931-30935	NN	denotes	lens
T7443	30936-30946	NN	denotes	epithelium
T7446	30947-30950	VBD	denotes	was
T7435	30951-30956	VBN	denotes	shown
T7447	30957-30959	TO	denotes	to
T7448	30960-30966	VB	denotes	induce
T7449	30967-30972	JJ	denotes	rapid
T7450	30973-30980	NNS	denotes	changes
T7451	30981-30983	IN	denotes	in
T7452	30984-30988	NN	denotes	cell
T7453	30989-30994	NN	denotes	shape
T7454	30994-30996	,	denotes	,
T7455	30996-31003	VBG	denotes	leading
T7456	31004-31006	IN	denotes	to
T7457	31007-31010	DT	denotes	the
T7458	31011-31020	NN	denotes	formation
T7459	31021-31023	IN	denotes	of
T7460	31024-31031	JJ	denotes	lentoid
T7461	31032-31038	NNS	denotes	bodies
T7462	31039-31040	-LRB-	denotes	[
T7463	31040-31042	CD	denotes	21
T7464	31042-31043	-RRB-	denotes	]
T7465	31043-31044	.	denotes	.
T7466	31044-31187	sentence	denotes	These studies strongly suggest that the hsp family of proteins has other functions in addition to protecting proteins and cells during stress.
T7467	31045-31050	DT	denotes	These
T7468	31051-31058	NNS	denotes	studies
T7470	31059-31067	RB	denotes	strongly
T7469	31068-31075	VBP	denotes	suggest
T7471	31076-31080	IN	denotes	that
T7473	31081-31084	DT	denotes	the
T7475	31085-31088	NN	denotes	hsp
T7474	31089-31095	NN	denotes	family
T7476	31096-31098	IN	denotes	of
T7477	31099-31107	NN	denotes	proteins
T7472	31108-31111	VBZ	denotes	has
T7478	31112-31117	JJ	denotes	other
T7479	31118-31127	NNS	denotes	functions
T7480	31128-31130	IN	denotes	in
T7481	31131-31139	NN	denotes	addition
T7482	31140-31142	IN	denotes	to
T7483	31143-31153	VBG	denotes	protecting
T7484	31154-31162	NN	denotes	proteins
T7485	31163-31166	CC	denotes	and
T7486	31167-31172	NNS	denotes	cells
T7487	31173-31179	IN	denotes	during
T7488	31180-31186	NN	denotes	stress
T7489	31186-31187	.	denotes	.
T7490	31187-31301	sentence	denotes	Alpha-crystallin may play a functional role in the cell nucleus and may have a role in regulating the cell cycle.
T7491	31188-31193	NN	denotes	Alpha
T7493	31193-31194	HYPH	denotes	-
T7492	31194-31204	NN	denotes	crystallin
T7495	31205-31208	MD	denotes	may
T7494	31209-31213	VB	denotes	play
T7496	31214-31215	DT	denotes	a
T7498	31216-31226	JJ	denotes	functional
T7497	31227-31231	NN	denotes	role
T7499	31232-31234	IN	denotes	in
T7500	31235-31238	DT	denotes	the
T7502	31239-31243	NN	denotes	cell
T7501	31244-31251	NN	denotes	nucleus
T7503	31252-31255	CC	denotes	and
T7504	31256-31259	MD	denotes	may
T7505	31260-31264	VB	denotes	have
T7506	31265-31266	DT	denotes	a
T7507	31267-31271	NN	denotes	role
T7508	31272-31274	IN	denotes	in
T7509	31275-31285	VBG	denotes	regulating
T7510	31286-31289	DT	denotes	the
T7512	31290-31294	NN	denotes	cell
T7511	31295-31300	NN	denotes	cycle
T7513	31300-31301	.	denotes	.
T7514	31301-31463	sentence	denotes	Several heat shock proteins have been found in cell nuclei in the absence of stress [30], and alpha-crystallin has been shown to interact directly with DNA [18].
T7515	31302-31309	JJ	denotes	Several
T7517	31310-31314	NN	denotes	heat
T7518	31315-31320	NN	denotes	shock
T7516	31321-31329	NN	denotes	proteins
T7520	31330-31334	VBP	denotes	have
T7521	31335-31339	VBN	denotes	been
T7519	31340-31345	VBN	denotes	found
T7522	31346-31348	IN	denotes	in
T7523	31349-31353	NN	denotes	cell
T7524	31354-31360	NNS	denotes	nuclei
T7525	31361-31363	IN	denotes	in
T7526	31364-31367	DT	denotes	the
T7527	31368-31375	NN	denotes	absence
T7528	31376-31378	IN	denotes	of
T7529	31379-31385	NN	denotes	stress
T7530	31386-31387	-LRB-	denotes	[
T7531	31387-31389	CD	denotes	30
T7532	31389-31390	-RRB-	denotes	]
T7533	31390-31392	,	denotes	,
T7534	31392-31395	CC	denotes	and
T7535	31396-31401	NN	denotes	alpha
T7537	31401-31402	HYPH	denotes	-
T7536	31402-31412	NN	denotes	crystallin
T7539	31413-31416	VBZ	denotes	has
T7540	31417-31421	VBN	denotes	been
T7538	31422-31427	VBN	denotes	shown
T7541	31428-31430	TO	denotes	to
T7542	31431-31439	VB	denotes	interact
T7543	31440-31448	RB	denotes	directly
T7544	31449-31453	IN	denotes	with
T7545	31454-31457	NN	denotes	DNA
T7546	31458-31459	-LRB-	denotes	[
T7547	31459-31461	CD	denotes	18
T7548	31461-31462	-RRB-	denotes	]
T7549	31462-31463	.	denotes	.
T7550	31463-31635	sentence	denotes	AlphaB, expressed in transfected CHO cells, has been shown to ectopically localize to interphase nuclei, suggesting a regulatory role for this protein in the nucleus [19].
T7551	31464-31470	NN	denotes	AlphaB
T7553	31470-31472	,	denotes	,
T7554	31472-31481	VBN	denotes	expressed
T7555	31482-31484	IN	denotes	in
T7556	31485-31496	VBN	denotes	transfected
T7558	31497-31500	NN	denotes	CHO
T7557	31501-31506	NNS	denotes	cells
T7559	31506-31508	,	denotes	,
T7560	31508-31511	VBZ	denotes	has
T7561	31512-31516	VBN	denotes	been
T7552	31517-31522	VBN	denotes	shown
T7562	31523-31525	TO	denotes	to
T7564	31526-31537	RB	denotes	ectopically
T7563	31538-31546	VB	denotes	localize
T7565	31547-31549	IN	denotes	to
T7566	31550-31560	NN	denotes	interphase
T7567	31561-31567	NNS	denotes	nuclei
T7568	31567-31569	,	denotes	,
T7569	31569-31579	VBG	denotes	suggesting
T7570	31580-31581	DT	denotes	a
T7572	31582-31592	JJ	denotes	regulatory
T7571	31593-31597	NN	denotes	role
T7573	31598-31601	IN	denotes	for
T7574	31602-31606	DT	denotes	this
T7575	31607-31614	NN	denotes	protein
T7576	31615-31617	IN	denotes	in
T7577	31618-31621	DT	denotes	the
T7578	31622-31629	NN	denotes	nucleus
T7579	31630-31631	-LRB-	denotes	[
T7580	31631-31633	CD	denotes	19
T7581	31633-31634	-RRB-	denotes	]
T7582	31634-31635	.	denotes	.
T7583	31635-31816	sentence	denotes	A subset of immortalized lens epithelial cells from alphaBKO mice have been shown to hyperproliferate [20] suggesting that alphaB may be important in maintaining genomic stability.
T7584	31636-31637	DT	denotes	A
T7585	31638-31644	NN	denotes	subset
T7587	31645-31647	IN	denotes	of
T7588	31648-31660	VBN	denotes	immortalized
T7590	31661-31665	NN	denotes	lens
T7591	31666-31676	JJ	denotes	epithelial
T7589	31677-31682	NNS	denotes	cells
T7592	31683-31687	IN	denotes	from
T7593	31688-31696	NN	denotes	alphaBKO
T7594	31697-31701	NNS	denotes	mice
T7595	31702-31706	VBP	denotes	have
T7596	31707-31711	VBN	denotes	been
T7586	31712-31717	VBN	denotes	shown
T7597	31718-31720	TO	denotes	to
T7598	31721-31737	VB	denotes	hyperproliferate
T7599	31738-31739	-LRB-	denotes	[
T7600	31739-31741	CD	denotes	20
T7601	31741-31742	-RRB-	denotes	]
T7602	31743-31753	VBG	denotes	suggesting
T7603	31754-31758	IN	denotes	that
T7605	31759-31765	NN	denotes	alphaB
T7606	31766-31769	MD	denotes	may
T7604	31770-31772	VB	denotes	be
T7607	31773-31782	JJ	denotes	important
T7608	31783-31785	IN	denotes	in
T7609	31786-31797	VBG	denotes	maintaining
T7610	31798-31805	JJ	denotes	genomic
T7611	31806-31815	NN	denotes	stability
T7612	31815-31816	.	denotes	.
T7613	31816-32000	sentence	denotes	In lens epithelium derived from alphaAKO lenses, cell growth rates were reported to be 50% lower compared to wild type [31], suggesting a role for alphaA in regulating the cell cycle.
T7614	31817-31819	IN	denotes	In
T7616	31820-31824	NN	denotes	lens
T7617	31825-31835	NN	denotes	epithelium
T7618	31836-31843	VBN	denotes	derived
T7619	31844-31848	IN	denotes	from
T7620	31849-31857	NN	denotes	alphaAKO
T7621	31858-31864	NNS	denotes	lenses
T7622	31864-31866	,	denotes	,
T7623	31866-31870	NN	denotes	cell
T7624	31871-31877	NN	denotes	growth
T7625	31878-31883	NNS	denotes	rates
T7626	31884-31888	VBD	denotes	were
T7615	31889-31897	VBN	denotes	reported
T7627	31898-31900	TO	denotes	to
T7628	31901-31903	VB	denotes	be
T7629	31904-31906	CD	denotes	50
T7630	31906-31907	NN	denotes	%
T7631	31908-31913	JJR	denotes	lower
T7632	31914-31922	VBN	denotes	compared
T7633	31923-31925	IN	denotes	to
T7634	31926-31930	JJ	denotes	wild
T7635	31931-31935	NN	denotes	type
T7636	31936-31937	-LRB-	denotes	[
T7637	31937-31939	CD	denotes	31
T7638	31939-31940	-RRB-	denotes	]
T7639	31940-31942	,	denotes	,
T7640	31942-31952	VBG	denotes	suggesting
T7641	31953-31954	DT	denotes	a
T7642	31955-31959	NN	denotes	role
T7643	31960-31963	IN	denotes	for
T7644	31964-31970	NN	denotes	alphaA
T7645	31971-31973	IN	denotes	in
T7646	31974-31984	VBG	denotes	regulating
T7647	31985-31988	DT	denotes	the
T7649	31989-31993	NN	denotes	cell
T7648	31994-31999	NN	denotes	cycle
T7650	31999-32000	.	denotes	.
T7651	32000-32158	sentence	denotes	All of these findings raise many questions as to the possible role(s) of alpha-crystallin in the nucleus and in cell cycle regulation during differentiation.
T7652	32001-32004	DT	denotes	All
T7654	32005-32007	IN	denotes	of
T7655	32008-32013	DT	denotes	these
T7656	32014-32022	NNS	denotes	findings
T7653	32023-32028	VBP	denotes	raise
T7657	32029-32033	JJ	denotes	many
T7658	32034-32043	NNS	denotes	questions
T7659	32044-32046	IN	denotes	as
T7660	32047-32049	IN	denotes	to
T7661	32050-32053	DT	denotes	the
T7663	32054-32062	JJ	denotes	possible
T7664	32063-32067	NN	denotes	role
T7665	32067-32068	-LRB-	denotes	(
T7662	32068-32069	NNS	denotes	s
T7666	32069-32070	-RRB-	denotes	)
T7667	32071-32073	IN	denotes	of
T7668	32074-32079	NN	denotes	alpha
T7670	32079-32080	HYPH	denotes	-
T7669	32080-32090	NN	denotes	crystallin
T7671	32091-32093	IN	denotes	in
T7672	32094-32097	DT	denotes	the
T7673	32098-32105	NN	denotes	nucleus
T7674	32106-32109	CC	denotes	and
T7675	32110-32112	IN	denotes	in
T7676	32113-32117	NN	denotes	cell
T7677	32118-32123	NN	denotes	cycle
T7678	32124-32134	NN	denotes	regulation
T7679	32135-32141	IN	denotes	during
T7680	32142-32157	NN	denotes	differentiation
T7681	32157-32158	.	denotes	.
T7682	32158-32455	sentence	denotes	In the current study, the observation of a disorganized pattern of nuclei localized to the equatorial bow region of alphaA/BKO lenses, and nucleic acid staining of structures throughout the anterior cortex of 54 wk alphaA/BKO lenses, is consistent with a role for alpha-crystallin in the nucleus.
T7683	32159-32161	IN	denotes	In
T7685	32162-32165	DT	denotes	the
T7687	32166-32173	JJ	denotes	current
T7686	32174-32179	NN	denotes	study
T7688	32179-32181	,	denotes	,
T7689	32181-32184	DT	denotes	the
T7690	32185-32196	NN	denotes	observation
T7691	32197-32199	IN	denotes	of
T7692	32200-32201	DT	denotes	a
T7694	32202-32214	JJ	denotes	disorganized
T7693	32215-32222	NN	denotes	pattern
T7695	32223-32225	IN	denotes	of
T7696	32226-32232	NNS	denotes	nuclei
T7697	32233-32242	VBN	denotes	localized
T7698	32243-32245	IN	denotes	to
T7699	32246-32249	DT	denotes	the
T7701	32250-32260	JJ	denotes	equatorial
T7702	32261-32264	NN	denotes	bow
T7700	32265-32271	NN	denotes	region
T7703	32272-32274	IN	denotes	of
T7704	32275-32281	NN	denotes	alphaA
T7706	32281-32282	HYPH	denotes	/
T7705	32282-32285	NN	denotes	BKO
T7707	32286-32292	NNS	denotes	lenses
T7708	32292-32294	,	denotes	,
T7709	32294-32297	CC	denotes	and
T7710	32298-32305	JJ	denotes	nucleic
T7711	32306-32310	NN	denotes	acid
T7712	32311-32319	NN	denotes	staining
T7713	32320-32322	IN	denotes	of
T7714	32323-32333	NNS	denotes	structures
T7715	32334-32344	IN	denotes	throughout
T7716	32345-32348	DT	denotes	the
T7718	32349-32357	JJ	denotes	anterior
T7717	32358-32364	NN	denotes	cortex
T7719	32365-32367	IN	denotes	of
T7720	32368-32370	CD	denotes	54
T7721	32371-32373	NN	denotes	wk
T7723	32374-32380	NN	denotes	alphaA
T7725	32380-32381	HYPH	denotes	/
T7724	32381-32384	NN	denotes	BKO
T7722	32385-32391	NNS	denotes	lenses
T7726	32391-32393	,	denotes	,
T7684	32393-32395	VBZ	denotes	is
T7727	32396-32406	JJ	denotes	consistent
T7728	32407-32411	IN	denotes	with
T7729	32412-32413	DT	denotes	a
T7730	32414-32418	NN	denotes	role
T7731	32419-32422	IN	denotes	for
T7732	32423-32428	NN	denotes	alpha
T7734	32428-32429	HYPH	denotes	-
T7733	32429-32439	NN	denotes	crystallin
T7735	32440-32442	IN	denotes	in
T7736	32443-32446	DT	denotes	the
T7737	32447-32454	NN	denotes	nucleus
T7738	32454-32455	.	denotes	.
T7739	32455-32588	sentence	denotes	There is extensive evidence from previous studies demonstrating that alpha-crystallin plays a role in the cytoskeletal organization.
T7740	32456-32461	EX	denotes	There
T7741	32462-32464	VBZ	denotes	is
T7742	32465-32474	JJ	denotes	extensive
T7743	32475-32483	NN	denotes	evidence
T7744	32484-32488	IN	denotes	from
T7745	32489-32497	JJ	denotes	previous
T7746	32498-32505	NNS	denotes	studies
T7747	32506-32519	VBG	denotes	demonstrating
T7748	32520-32524	IN	denotes	that
T7750	32525-32530	NN	denotes	alpha
T7752	32530-32531	HYPH	denotes	-
T7751	32531-32541	NN	denotes	crystallin
T7749	32542-32547	VBZ	denotes	plays
T7753	32548-32549	DT	denotes	a
T7754	32550-32554	NN	denotes	role
T7755	32555-32557	IN	denotes	in
T7756	32558-32561	DT	denotes	the
T7758	32562-32574	JJ	denotes	cytoskeletal
T7757	32575-32587	NN	denotes	organization
T7759	32587-32588	.	denotes	.
T7760	32588-32680	sentence	denotes	Both alphaA and alphaB can bind specifically to actin, both in vitro [13] and in vivo [14].
T7761	32589-32593	CC	denotes	Both
T7762	32594-32600	NN	denotes	alphaA
T7764	32601-32604	CC	denotes	and
T7765	32605-32611	NN	denotes	alphaB
T7766	32612-32615	MD	denotes	can
T7763	32616-32620	VB	denotes	bind
T7767	32621-32633	RB	denotes	specifically
T7768	32634-32636	IN	denotes	to
T7769	32637-32642	NN	denotes	actin
T7770	32642-32644	,	denotes	,
T7771	32644-32648	CC	denotes	both
T7773	32649-32651	FW	denotes	in
T7774	32652-32657	FW	denotes	vitro
T7775	32658-32659	-LRB-	denotes	[
T7772	32659-32661	CD	denotes	13
T7776	32661-32662	-RRB-	denotes	]
T7777	32663-32666	CC	denotes	and
T7778	32667-32669	FW	denotes	in
T7780	32670-32674	FW	denotes	vivo
T7781	32675-32676	-LRB-	denotes	[
T7779	32676-32678	CD	denotes	14
T7782	32678-32679	-RRB-	denotes	]
T7783	32679-32680	.	denotes	.
T7784	32680-32893	sentence	denotes	Actin filament formation has been shown to be necessary for the differentiation of lens epithelial cells [15], however, the significance of alpha-crystallin interaction with actin in differentiation is not known.
T7785	32681-32686	NN	denotes	Actin
T7786	32687-32695	NN	denotes	filament
T7787	32696-32705	NN	denotes	formation
T7789	32706-32709	VBZ	denotes	has
T7790	32710-32714	VBN	denotes	been
T7788	32715-32720	VBN	denotes	shown
T7792	32721-32723	TO	denotes	to
T7793	32724-32726	VB	denotes	be
T7794	32727-32736	JJ	denotes	necessary
T7795	32737-32740	IN	denotes	for
T7796	32741-32744	DT	denotes	the
T7797	32745-32760	NN	denotes	differentiation
T7798	32761-32763	IN	denotes	of
T7799	32764-32768	NN	denotes	lens
T7801	32769-32779	JJ	denotes	epithelial
T7800	32780-32785	NNS	denotes	cells
T7802	32786-32787	-LRB-	denotes	[
T7803	32787-32789	CD	denotes	15
T7804	32789-32790	-RRB-	denotes	]
T7805	32790-32792	,	denotes	,
T7806	32792-32799	RB	denotes	however
T7807	32799-32801	,	denotes	,
T7808	32801-32804	DT	denotes	the
T7809	32805-32817	NN	denotes	significance
T7810	32818-32820	IN	denotes	of
T7811	32821-32826	NN	denotes	alpha
T7813	32826-32827	HYPH	denotes	-
T7812	32827-32837	NN	denotes	crystallin
T7814	32838-32849	NN	denotes	interaction
T7815	32850-32854	IN	denotes	with
T7816	32855-32860	NN	denotes	actin
T7817	32861-32863	IN	denotes	in
T7818	32864-32879	NN	denotes	differentiation
T7819	32880-32882	VBZ	denotes	is
T7820	32883-32886	RB	denotes	not
T7791	32887-32892	VBN	denotes	known
T7821	32892-32893	.	denotes	.
T7822	32893-33109	sentence	denotes	In the lens, alpha-crystallin also forms a complex with type III intermediate filament proteins and the lens-specific beaded filament proteins CP49 and CP115, which may be critical for proper filament assembly [16].
T7823	32894-32896	IN	denotes	In
T7825	32897-32900	DT	denotes	the
T7826	32901-32905	NN	denotes	lens
T7827	32905-32907	,	denotes	,
T7828	32907-32912	NN	denotes	alpha
T7830	32912-32913	HYPH	denotes	-
T7829	32913-32923	NN	denotes	crystallin
T7831	32924-32928	RB	denotes	also
T7824	32929-32934	VBZ	denotes	forms
T7832	32935-32936	DT	denotes	a
T7833	32937-32944	NN	denotes	complex
T7834	32945-32949	IN	denotes	with
T7835	32950-32954	NN	denotes	type
T7837	32955-32958	CD	denotes	III
T7838	32959-32971	JJ	denotes	intermediate
T7839	32972-32980	NN	denotes	filament
T7836	32981-32989	NN	denotes	proteins
T7840	32990-32993	CC	denotes	and
T7841	32994-32997	DT	denotes	the
T7843	32998-33002	NN	denotes	lens
T7845	33002-33003	HYPH	denotes	-
T7844	33003-33011	JJ	denotes	specific
T7846	33012-33018	VBN	denotes	beaded
T7847	33019-33027	NN	denotes	filament
T7842	33028-33036	NN	denotes	proteins
T7848	33037-33041	NN	denotes	CP49
T7849	33042-33045	CC	denotes	and
T7850	33046-33051	NN	denotes	CP115
T7851	33051-33053	,	denotes	,
T7852	33053-33058	WDT	denotes	which
T7854	33059-33062	MD	denotes	may
T7853	33063-33065	VB	denotes	be
T7855	33066-33074	JJ	denotes	critical
T7856	33075-33078	IN	denotes	for
T7857	33079-33085	JJ	denotes	proper
T7859	33086-33094	NN	denotes	filament
T7858	33095-33103	NN	denotes	assembly
T7860	33104-33105	-LRB-	denotes	[
T7861	33105-33107	CD	denotes	16
T7862	33107-33108	-RRB-	denotes	]
T7863	33108-33109	.	denotes	.
T7864	33109-33272	sentence	denotes	Beaded filament mRNA levels increase greatly in differentiating lens epithelial cells, and have been suggested as a pan-specific marker for lens fiber cells [17].
T7865	33110-33116	VBN	denotes	Beaded
T7866	33117-33125	NN	denotes	filament
T7868	33126-33130	NN	denotes	mRNA
T7867	33131-33137	NNS	denotes	levels
T7869	33138-33146	VBP	denotes	increase
T7870	33147-33154	RB	denotes	greatly
T7871	33155-33157	IN	denotes	in
T7872	33158-33173	VBG	denotes	differentiating
T7874	33174-33178	NN	denotes	lens
T7875	33179-33189	JJ	denotes	epithelial
T7873	33190-33195	NNS	denotes	cells
T7876	33195-33197	,	denotes	,
T7877	33197-33200	CC	denotes	and
T7878	33201-33205	VBP	denotes	have
T7880	33206-33210	VBN	denotes	been
T7879	33211-33220	VBN	denotes	suggested
T7881	33221-33223	IN	denotes	as
T7882	33224-33225	DT	denotes	a
T7884	33226-33238	JJ	denotes	pan-specific
T7883	33239-33245	NN	denotes	marker
T7885	33246-33249	IN	denotes	for
T7886	33250-33254	NN	denotes	lens
T7887	33255-33260	NN	denotes	fiber
T7888	33261-33266	NNS	denotes	cells
T7889	33267-33268	-LRB-	denotes	[
T7890	33268-33270	CD	denotes	17
T7891	33270-33271	-RRB-	denotes	]
T7892	33271-33272	.	denotes	.
T7893	33272-33510	sentence	denotes	It is therefore possible that increased synthesis of alpha-crystallin in epithelial cells early in the differentiation process may have profound effects upon the cytoskeleton, which in turn may profoundly affect cell shape and migration.
T7894	33273-33275	PRP	denotes	It
T7895	33276-33278	VBZ	denotes	is
T7896	33279-33288	RB	denotes	therefore
T7897	33289-33297	JJ	denotes	possible
T7898	33298-33302	IN	denotes	that
T7900	33303-33312	VBN	denotes	increased
T7901	33313-33322	NN	denotes	synthesis
T7902	33323-33325	IN	denotes	of
T7903	33326-33331	NN	denotes	alpha
T7905	33331-33332	HYPH	denotes	-
T7904	33332-33342	NN	denotes	crystallin
T7906	33343-33345	IN	denotes	in
T7907	33346-33356	JJ	denotes	epithelial
T7908	33357-33362	NNS	denotes	cells
T7909	33363-33368	RB	denotes	early
T7910	33369-33371	IN	denotes	in
T7911	33372-33375	DT	denotes	the
T7913	33376-33391	NN	denotes	differentiation
T7912	33392-33399	NN	denotes	process
T7914	33400-33403	MD	denotes	may
T7899	33404-33408	VB	denotes	have
T7915	33409-33417	JJ	denotes	profound
T7916	33418-33425	NNS	denotes	effects
T7917	33426-33430	IN	denotes	upon
T7918	33431-33434	DT	denotes	the
T7919	33435-33447	NN	denotes	cytoskeleton
T7920	33447-33449	,	denotes	,
T7921	33449-33454	WDT	denotes	which
T7923	33455-33457	IN	denotes	in
T7924	33458-33462	NN	denotes	turn
T7925	33463-33466	MD	denotes	may
T7926	33467-33477	RB	denotes	profoundly
T7922	33478-33484	VB	denotes	affect
T7927	33485-33489	NN	denotes	cell
T7928	33490-33495	NN	denotes	shape
T7929	33496-33499	CC	denotes	and
T7930	33500-33509	NN	denotes	migration
T7931	33509-33510	.	denotes	.
T7932	33510-33646	sentence	denotes	The lack of cellar organization and uniform cell shape at the equatorial region observed in alphaA/BKO lenses supports this hypothesis.
T7933	33511-33514	DT	denotes	The
T7934	33515-33519	NN	denotes	lack
T7936	33520-33522	IN	denotes	of
T7937	33523-33529	JJ	denotes	cellar
T7938	33530-33542	NN	denotes	organization
T7939	33543-33546	CC	denotes	and
T7940	33547-33554	JJ	denotes	uniform
T7942	33555-33559	NN	denotes	cell
T7941	33560-33565	NN	denotes	shape
T7943	33566-33568	IN	denotes	at
T7944	33569-33572	DT	denotes	the
T7946	33573-33583	JJ	denotes	equatorial
T7945	33584-33590	NN	denotes	region
T7947	33591-33599	VBN	denotes	observed
T7948	33600-33602	IN	denotes	in
T7949	33603-33609	NN	denotes	alphaA
T7951	33609-33610	HYPH	denotes	/
T7950	33610-33613	NN	denotes	BKO
T7952	33614-33620	NNS	denotes	lenses
T7935	33621-33629	VBZ	denotes	supports
T7953	33630-33634	DT	denotes	this
T7954	33635-33645	NN	denotes	hypothesis
T7955	33645-33646	.	denotes	.
T7956	33646-33743	sentence	denotes	Studies are currently underway to characterize cytoskeletal organization in the alphaA/BKO lens.
T7957	33647-33654	NNS	denotes	Studies
T7958	33655-33658	VBP	denotes	are
T7959	33659-33668	RB	denotes	currently
T7960	33669-33677	JJ	denotes	underway
T7961	33678-33680	TO	denotes	to
T7962	33681-33693	VB	denotes	characterize
T7963	33694-33706	JJ	denotes	cytoskeletal
T7964	33707-33719	NN	denotes	organization
T7965	33720-33722	IN	denotes	in
T7966	33723-33726	DT	denotes	the
T7968	33727-33733	NN	denotes	alphaA
T7970	33733-33734	HYPH	denotes	/
T7969	33734-33737	NN	denotes	BKO
T7967	33738-33742	NN	denotes	lens
T7971	33742-33743	.	denotes	.
T7972	34214-34217	DT	denotes	The
T7974	34218-34233	JJ	denotes	pre-publication
T7973	34234-34241	NN	denotes	history
T7976	34242-34245	IN	denotes	for
T7977	34246-34250	DT	denotes	this
T7978	34251-34256	NN	denotes	paper
T7979	34257-34260	MD	denotes	can
T7980	34261-34263	VB	denotes	be
T7975	34264-34272	VBN	denotes	accessed
T7981	34273-34277	RB	denotes	here
T7982	34277-34278	:	denotes	:
R10	T142	T140	pobj	lens,of
R100	T241	T239	prep	of,purpose
R1000	T1540	T1541	nummod	One,approach
R1001	T1645	T1644	neg	not,was
R1002	T1646	T1644	acomp	possible,was
R1003	T1647	T1644	advcl	using,was
R1004	T1541	T1542	nsubj	approach,is
R1005	T1648	T1649	det	the,techniques
R1006	T1649	T1647	dobj	techniques,using
R1007	T1650	T1649	acl	employed,techniques
R1008	T1543	T1541	prep	to,approach
R1009	T1651	T1650	prep	in,employed
R101	T242	T243	det	this,study
R1010	T1652	T1653	det	the,study
R1011	T1653	T1651	pobj	study,in
R1012	T1654	T1655	aux	to,determine
R1013	T1544	T1543	pcomp	resolving,to
R1014	T1655	T1644	xcomp	determine,was
R1015	T1656	T1657	mark	if,were
R1016	T1657	T1655	ccomp	were,determine
R1017	T1658	T1659	det	the,lenses
R1018	T1659	T1657	nsubj	lenses,were
R1019	T1660	T1659	amod	smaller,lenses
R102	T243	T241	pobj	study,of
R1020	T1545	T1544	dobj	some,resolving
R1021	T1661	T1657	prep	due,were
R1022	T1662	T1661	pcomp	to,due
R1023	T1663	T1664	amod	reduced,volume
R1024	T1664	T1661	pobj	volume,due
R1025	T1546	T1545	prep	of,some
R1026	T1665	T1664	cc	or,volume
R1027	T1666	T1664	conj	number,volume
R1028	T1667	T1666	prep	of,number
R1029	T1547	T1548	det	the,functions
R103	T244	T245	aux	to,characterize
R1030	T1668	T1669	compound	fiber,cells
R1031	T1669	T1667	pobj	cells,of
R1032	T1548	T1546	pobj	functions,of
R1033	T1670	T1644	punct	.,was
R1034	T1672	T1673	amod	Targeted,disruption
R1035	T1549	T1550	advmod	in,vivo
R1036	T1673	T1674	nsubj	disruption,resulted
R1037	T1675	T1673	prep	of,disruption
R1038	T1550	T1548	amod	vivo,functions
R1039	T1676	T1677	det	the,gene
R104	T245	T240	xcomp	characterize,was
R1040	T1677	T1675	pobj	gene,of
R1041	T1678	T1677	compound	mouse,gene
R1042	T1551	T1548	prep	of,functions
R1043	T1679	T1677	compound	alphaB,gene
R1044	T1680	T1674	prep	in,resulted
R1045	T1552	T1553	compound	alpha,crystallin
R1046	T1681	T1680	pobj	lenses,in
R1047	T1682	T1681	amod	similar,lenses
R1048	T1683	T1682	prep	in,similar
R1049	T1553	T1551	pobj	crystallin,of
R105	T246	T247	amod	gross,morphology
R1050	T1684	T1683	pobj	size,in
R1051	T1685	T1682	prep	to,similar
R1052	T1686	T1687	npadvmod	aged,matched
R1053	T1554	T1553	punct	-,crystallin
R1054	T1687	T1689	amod	matched,lens
R1055	T1688	T1687	punct	-,matched
R1056	T1689	T1685	pobj	lens,to
R1057	T1690	T1691	amod	wild,type
R1058	T1555	T1556	aux	to,generate
R1059	T1691	T1689	compound	type,lens
R106	T247	T245	dobj	morphology,characterize
R1060	T1556	T1542	xcomp	generate,is
R1061	T1692	T1674	punct	.,resulted
R1062	T1557	T1558	compound	animal,models
R1063	T1694	T1695	advmod	Moreover,observed
R1064	T1558	T1556	dobj	models,generate
R1065	T1696	T1695	punct	", ",observed
R1066	T1697	T1698	det	no,formation
R1067	T1698	T1695	nsubjpass	formation,observed
R1068	T1559	T1560	advmod	where,eliminated
R1069	T1699	T1698	compound	cataract,formation
R107	T248	T247	amod	lenticular,morphology
R1070	T1700	T1695	auxpass	was,observed
R1071	T1701	T1695	prep	in,observed
R1072	T1702	T1703	det	the,lenses
R1073	T1703	T1701	pobj	lenses,in
R1074	T1560	T1558	relcl	eliminated,models
R1075	T1704	T1705	compound	alphaB,knockout
R1076	T1705	T1703	compound	knockout,lenses
R1077	T1706	T1695	punct	.,observed
R1078	T1561	T1560	nsubjpass	one,eliminated
R1079	T1708	T1709	det	These,animals
R108	T249	T245	prep	in,characterize
R1080	T1709	T1710	nsubj	animals,have
R1081	T1562	T1561	cc	or,one
R1082	T1711	T1712	compound	muscle,cell
R1083	T1712	T1713	compound	cell,abnormalities
R1084	T1563	T1561	conj	both,one
R1085	T1713	T1710	dobj	abnormalities,have
R1086	T1714	T1713	punct	", ",abnormalities
R1087	T1564	T1561	prep	of,one
R1088	T1565	T1566	det	the,products
R1089	T1566	T1564	pobj	products,of
R109	T250	T251	amod	normal,mice
R1090	T1567	T1568	compound	alpha,crystallin
R1091	T1715	T1716	amod	severe,anomalies
R1092	T1716	T1713	conj	anomalies,abnormalities
R1093	T1568	T1566	compound	crystallin,products
R1094	T1717	T1716	amod	postural,anomalies
R1095	T1718	T1716	punct	", ",anomalies
R1096	T1569	T1568	punct	-,crystallin
R1097	T1719	T1720	amod	selective,degeneration
R1098	T1720	T1716	conj	degeneration,anomalies
R1099	T1721	T1720	compound	muscle,degeneration
R11	T143	T144	nmod	AlphaA,crystallin
R110	T251	T249	pobj	mice,in
R1100	T1570	T1566	compound	gene,products
R1101	T1722	T1720	punct	", ",degeneration
R1102	T1723	T1720	cc	and,degeneration
R1103	T1724	T1725	amod	shorter,spans
R1104	T1725	T1720	conj	spans,degeneration
R1105	T1571	T1560	aux	have,eliminated
R1106	T1726	T1725	compound	life,spans
R1107	T1727	T1713	acl	compared,abnormalities
R1108	T1572	T1560	auxpass	been,eliminated
R1109	T1728	T1727	prep	to,compared
R111	T252	T251	cc	and,mice
R1110	T1729	T1730	amod	normal,controls
R1111	T1730	T1728	pobj	controls,to
R1112	T1573	T1542	punct	.,is
R1113	T1731	T1732	punct	[,23
R1114	T1732	T1710	parataxis	23,have
R1115	T1733	T1732	punct	],23
R1116	T1575	T1576	nsubj	Brady,demonstrated
R1117	T1734	T1710	punct	.,have
R1118	T1736	T1737	prep	In,compensate
R1119	T1577	T1578	advmod	et,al.
R112	T253	T251	conj	mice,mice
R1120	T1738	T1739	det	the,mice
R1121	T1739	T1736	pobj	mice,In
R1122	T1740	T1741	amod	single,knockout
R1123	T1741	T1739	compound	knockout,mice
R1124	T1742	T1743	compound	alpha,crystallin
R1125	T1743	T1741	compound	crystallin,knockout
R1126	T1744	T1743	punct	-,crystallin
R1127	T1578	T1575	advmod	al.,Brady
R1128	T1745	T1737	punct	", ",compensate
R1129	T1746	T1747	det	the,crystallin
R113	T254	T253	prep	with,mice
R1130	T1747	T1737	nsubj	crystallin,compensate
R1131	T1579	T1580	punct	[,22
R1132	T1748	T1747	amod	remaining,crystallin
R1133	T1749	T1747	compound	alpha,crystallin
R1134	T1750	T1747	punct	-,crystallin
R1135	T1580	T1575	parataxis	22,Brady
R1136	T1751	T1737	aux	may,compensate
R1137	T1752	T1737	advmod	fully,compensate
R1138	T1581	T1580	punct	],22
R1139	T1753	T1752	cc	or,fully
R114	T255	T256	det	the,disruption
R1140	T1754	T1752	conj	partially,fully
R1141	T1755	T1737	prep	for,compensate
R1142	T1756	T1755	pobj	some,for
R1143	T1582	T1576	punct	", ",demonstrated
R1144	T1757	T1756	prep	of,some
R1145	T1758	T1759	det	the,functions
R1146	T1583	T1576	prep	by,demonstrated
R1147	T1759	T1757	pobj	functions,of
R1148	T1760	T1759	prep	of,functions
R1149	T1761	T1762	det	the,protein
R115	T256	T254	pobj	disruption,with
R1150	T1584	T1585	amod	targeted,disruption
R1151	T1762	T1760	pobj	protein,of
R1152	T1763	T1762	amod	missing,protein
R1153	T1585	T1583	pobj	disruption,by
R1154	T1764	T1737	punct	", ",compensate
R1155	T1765	T1766	advmod	especially,in
R1156	T1766	T1737	prep	in,compensate
R1157	T1586	T1585	prep	of,disruption
R1158	T1767	T1768	det	the,lens
R1159	T1768	T1766	pobj	lens,in
R116	T257	T256	amod	targeted,disruption
R1160	T1587	T1588	det	the,gene
R1161	T1769	T1768	punct	", ",lens
R1162	T1770	T1771	advmod	where,expressed
R1163	T1771	T1768	relcl	expressed,lens
R1164	T1588	T1586	pobj	gene,of
R1165	T1772	T1773	preconj	both,alphaA
R1166	T1773	T1771	nsubjpass	alphaA,expressed
R1167	T1774	T1773	cc	and,alphaA
R1168	T1775	T1773	conj	alphaB,alphaA
R1169	T1589	T1588	compound	mouse,gene
R117	T258	T256	prep	of,disruption
R1170	T1776	T1771	auxpass	are,expressed
R1171	T1777	T1771	advmod	normally,expressed
R1172	T1590	T1588	compound	alphaA,gene
R1173	T1778	T1771	prep	at,expressed
R1174	T1779	T1780	amod	high,levels
R1175	T1591	T1576	punct	", ",demonstrated
R1176	T1780	T1778	pobj	levels,at
R1177	T1781	T1737	punct	.,compensate
R1178	T1592	T1593	mark	that,was
R1179	T1783	T1784	det	The,objectives
R118	T259	T260	nmod	alphaA,crystallin
R1180	T1784	T1785	nsubj	objectives,were
R1181	T1593	T1576	ccomp	was,demonstrated
R1182	T1786	T1784	prep	of,objectives
R1183	T1787	T1788	det	the,report
R1184	T1788	T1786	pobj	report,of
R1185	T1594	T1595	det	this,protein
R1186	T1789	T1788	amod	current,report
R1187	T1790	T1791	aux	to,characterize
R1188	T1595	T1593	nsubj	protein,was
R1189	T1791	T1785	xcomp	characterize,were
R119	T260	T265	compound	crystallin,genes
R1190	T1792	T1793	amod	gross,morphology
R1191	T1793	T1791	dobj	morphology,characterize
R1192	T1596	T1593	acomp	essential,was
R1193	T1794	T1793	prep	of,morphology
R1194	T1795	T1796	amod	young,lenses
R1195	T1796	T1794	pobj	lenses,of
R1196	T1597	T1596	prep	for,essential
R1197	T1797	T1798	punct	(,wk
R1198	T1798	T1795	parataxis	wk,young
R1199	T1799	T1798	nummod	5,wk
R12	T144	T142	nmod	crystallin,lens
R120	T261	T259	punct	-,alphaA
R1200	T1598	T1599	det	the,maintenance
R1201	T1800	T1798	punct	),wk
R1202	T1801	T1795	cc	and,young
R1203	T1599	T1597	pobj	maintenance,for
R1204	T1802	T1795	conj	old,young
R1205	T1803	T1804	punct	(,wk
R1206	T1804	T1802	parataxis	wk,old
R1207	T1805	T1804	nummod	54,wk
R1208	T1600	T1599	prep	of,maintenance
R1209	T1806	T1804	punct	),wk
R121	T262	T259	cc	and,alphaA
R1210	T1807	T1796	compound	mouse,lenses
R1211	T1601	T1602	compound	lens,transparency
R1212	T1808	T1791	prep	with,characterize
R1213	T1809	T1810	amod	targeted,disruption
R1214	T1602	T1600	pobj	transparency,of
R1215	T1810	T1808	pobj	disruption,with
R1216	T1811	T1810	prep	of,disruption
R1217	T1812	T1813	preconj	both,genes
R1218	T1813	T1811	pobj	genes,of
R1219	T1603	T1593	punct	", ",was
R122	T263	T259	conj	alphaB,alphaA
R1220	T1814	T1813	det	the,genes
R1221	T1815	T1813	nmod	alphaA,genes
R1222	T1816	T1815	cc	and,alphaA
R1223	T1604	T1605	advmod	possibly,by
R1224	T1817	T1815	conj	alphaB,alphaA
R1225	T1818	T1791	punct	", ",characterize
R1226	T1605	T1593	prep	by,was
R1227	T1819	T1791	prep	in,characterize
R1228	T1820	T1819	pobj	comparison,in
R1229	T1606	T1605	pcomp	maintaining,by
R123	T264	T260	punct	-,crystallin
R1230	T1607	T1608	det	the,solubility
R1231	T1821	T1820	prep	to,comparison
R1232	T1822	T1823	npadvmod	age,matched
R1233	T1823	T1824	amod	matched,lenses
R1234	T1608	T1606	dobj	solubility,maintaining
R1235	T1824	T1821	pobj	lenses,to
R1236	T1825	T1826	amod	wild,type
R1237	T1826	T1824	compound	type,lenses
R1238	T1827	T1791	punct	", ",characterize
R1239	T1828	T1791	advcl	using,characterize
R124	T265	T258	pobj	genes,of
R1240	T1829	T1830	amod	scanning,microscopy
R1241	T1830	T1828	dobj	microscopy,using
R1242	T1831	T1830	compound	electron,microscopy
R1243	T1832	T1830	punct	(,microscopy
R1244	T1833	T1830	appos	SEM,microscopy
R1245	T1834	T1830	punct	),microscopy
R1246	T1835	T1830	cc	and,microscopy
R1247	T1836	T1837	amod	confocal,microscopy
R1248	T1837	T1830	conj	microscopy,microscopy
R1249	T1838	T1828	punct	", ",using
R125	T266	T265	punct	(,genes
R1250	T1839	T1840	aux	to,elucidate
R1251	T1840	T1828	advcl	elucidate,using
R1252	T1841	T1842	det	the,functions
R1253	T1842	T1840	dobj	functions,elucidate
R1254	T1843	T1842	amod	possible,functions
R1255	T1844	T1842	prep	of,functions
R1256	T1845	T1846	compound	alpha,crystallin
R1257	T1846	T1844	pobj	crystallin,of
R1258	T1847	T1846	punct	-,crystallin
R1259	T1848	T1842	prep	in,functions
R126	T267	T268	compound	alphaA,BKO
R1260	T1849	T1850	det	the,lens
R1261	T1850	T1848	pobj	lens,in
R1262	T1851	T1785	punct	.,were
R1263	T1853	T1854	det	The,results
R1264	T1854	T1855	nsubj	results,indicate
R1265	T1856	T1857	mark	that,is
R1266	T1857	T1855	ccomp	is,indicate
R1267	T1858	T1859	compound	alpha,crystallin
R1268	T1859	T1857	nsubj	crystallin,is
R1269	T1860	T1859	punct	-,crystallin
R127	T268	T265	parataxis	BKO,genes
R1270	T1861	T1857	acomp	necessary,is
R1271	T1862	T1861	prep	for,necessary
R1272	T1863	T1864	amod	proper,formation
R1273	T1864	T1862	pobj	formation,for
R1274	T1865	T1866	compound	fiber,cell
R1275	T1866	T1864	compound	cell,formation
R1276	T1867	T1864	cc	and,formation
R1277	T1868	T1869	amod	resulting,transparency
R1278	T1869	T1864	conj	transparency,formation
R1279	T1870	T1869	compound	lens,transparency
R128	T269	T268	punct	/,BKO
R1280	T1871	T1855	punct	.,indicate
R1281	T1953	T1954	det	The,strain
R1282	T1954	T1958	nsubj	strain,was
R1283	T1955	T1956	amod	wild,type
R1284	T1956	T1954	compound	type,strain
R1285	T1957	T1954	compound	mouse,strain
R1286	T1959	T1954	acl	used,strain
R1287	T1960	T1959	prep	in,used
R1288	T1961	T1962	det	this,study
R1289	T1962	T1960	pobj	study,in
R129	T270	T268	punct	),BKO
R1290	T1963	T1964	det	the,mouse
R1291	T1964	T1958	attr	mouse,was
R1292	T1965	T1964	compound	129SvEvTac,mouse
R1293	T1966	T1958	punct	.,was
R1294	T1968	T1969	nsubj	Lenses,were
R1295	T1970	T1968	acl	examined,Lenses
R1296	T1971	T1969	prep	from,were
R1297	T1972	T1973	nummod	5,wk
R1298	T1973	T1990	npadvmod	wk,old
R1299	T1974	T1975	punct	(,lenses
R13	T145	T143	punct	-,AlphaA
R130	T271	T240	punct	.,was
R1300	T1975	T1972	parataxis	lenses,5
R1301	T1976	T1975	nummod	8,lenses
R1302	T1977	T1975	punct	),lenses
R1303	T1978	T1972	punct	", ",5
R1304	T1979	T1972	conj	46,5
R1305	T1980	T1981	punct	(,lenses
R1306	T1981	T1979	parataxis	lenses,46
R1307	T1982	T1981	nummod	8,lenses
R1308	T1983	T1981	punct	),lenses
R1309	T1984	T1979	cc	and,46
R131	T275	T276	nsubjpass	Lenses,examined
R1310	T1985	T1979	conj	72,46
R1311	T1986	T1987	punct	(,lenses
R1312	T1987	T1985	parataxis	lenses,72
R1313	T1988	T1987	nummod	6,lenses
R1314	T1989	T1987	punct	),lenses
R1315	T1990	T1991	amod	old,mice
R1316	T1991	T1971	pobj	mice,from
R1317	T1992	T1969	punct	.,were
R1318	T1994	T1995	compound	AlphaA,BKO
R1319	T1995	T1997	nsubjpass	BKO,generated
R132	T277	T275	prep	from,Lenses
R1320	T1996	T1995	punct	/,BKO
R1321	T1998	T1997	auxpass	was,generated
R1322	T1999	T1997	prep	by,generated
R1323	T2000	T2001	dep	cross,breeding
R1324	T2001	T1999	pcomp	breeding,by
R1325	T2002	T2003	nmod	alphaAKO,127
R1326	T2003	T2005	nmod	127,mice
R1327	T2004	T2003	punct	-,127
R1328	T2005	T2001	dobj	mice,breeding
R1329	T2006	T2007	punct	[,22
R133	T278	T279	compound	129SvEvTac,mice
R1330	T2007	T2003	parataxis	22,127
R1331	T2008	T2007	punct	],22
R1332	T2009	T2003	cc	and,127
R1333	T2010	T2011	compound	alphaBKO,168
R1334	T2011	T2003	conj	168,127
R1335	T2012	T2011	punct	-,168
R1336	T2013	T2014	punct	[,23
R1337	T2014	T2005	parataxis	23,mice
R1338	T2015	T2014	punct	],23
R1339	T2016	T2001	punct	", ",breeding
R134	T279	T277	pobj	mice,from
R1340	T2017	T2018	advmod	also,in
R1341	T2018	T2001	prep	in,breeding
R1342	T2019	T2020	det	a,background
R1343	T2020	T2018	pobj	background,in
R1344	T2021	T2020	compound	129Sv,background
R1345	T2022	T1997	punct	.,generated
R1346	T2024	T2025	det	These,mice
R1347	T2025	T2026	nsubj	mice,lack
R1348	T2027	T2026	advmod	also,lack
R1349	T2028	T2029	det	the,product
R135	T280	T279	cc	and,mice
R1350	T2029	T2026	dobj	product,lack
R1351	T2030	T2029	compound	HSPB2,product
R1352	T2031	T2029	compound	gene,product
R1353	T2032	T2033	punct	[,23
R1354	T2033	T2026	parataxis	23,lack
R1355	T2034	T2033	punct	],23
R1356	T2035	T2026	punct	.,lack
R1357	T2037	T2038	nsubj	Lenses,were
R1358	T2039	T2037	acl	examined,Lenses
R1359	T2040	T2038	prep	from,were
R136	T281	T282	compound	alphaA,BKO
R1360	T2041	T2042	nummod	5,wk
R1361	T2042	T2043	npadvmod	wk,old
R1362	T2043	T2055	amod	old,mice
R1363	T2044	T2045	punct	(,lenses
R1364	T2045	T2042	parataxis	lenses,wk
R1365	T2046	T2045	nummod	6,lenses
R1366	T2047	T2045	punct	),lenses
R1367	T2048	T2042	cc	and,wk
R1368	T2049	T2050	nummod	54,wk
R1369	T2050	T2042	conj	wk,wk
R137	T282	T284	compound	BKO,mice
R1370	T2051	T2052	punct	(,lenses
R1371	T2052	T2050	parataxis	lenses,wk
R1372	T2053	T2052	nummod	16,lenses
R1373	T2054	T2052	punct	),lenses
R1374	T2055	T2040	pobj	mice,from
R1375	T2056	T2038	punct	.,were
R1376	T2058	T2059	det	All,animals
R1377	T2059	T2060	nsubjpass	animals,treated
R1378	T2061	T2060	auxpass	were,treated
R1379	T2062	T2060	prep	in,treated
R138	T283	T282	punct	/,BKO
R1380	T2063	T2062	pobj	accordance,in
R1381	T2064	T2063	prep	with,accordance
R1382	T2065	T2066	det	the,Statement
R1383	T2066	T2064	pobj	Statement,with
R1384	T2067	T2066	nmod	Association,Statement
R1385	T2068	T2067	prep	for,Association
R1386	T2069	T2068	pobj	Research,for
R1387	T2070	T2069	prep	in,Research
R1388	T2071	T2070	pobj	Vision,in
R1389	T2072	T2071	cc	and,Vision
R139	T284	T279	conj	mice,mice
R1390	T2073	T2071	conj	Ophthalmology,Vision
R1391	T2074	T2066	prep	for,Statement
R1392	T2075	T2076	det	the,Use
R1393	T2076	T2074	pobj	Use,for
R1394	T2077	T2076	prep	of,Use
R1395	T2078	T2077	pobj	Animals,of
R1396	T2079	T2076	prep	in,Use
R1397	T2080	T2081	nmod	Ophthalmic,Research
R1398	T2081	T2079	pobj	Research,in
R1399	T2082	T2080	cc	and,Ophthalmic
R14	T146	T143	cc	and,AlphaA
R140	T285	T276	auxpass	were,examined
R1400	T2083	T2080	conj	Vision,Ophthalmic
R1401	T2084	T2060	punct	.,treated
R1402	T2086	T2087	advmod	Immediately,after
R1403	T2087	T2088	prep	after,enucleated
R1404	T2089	T2087	pcomp	euthanizing,after
R1405	T2090	T2089	dobj	animals,euthanizing
R1406	T2091	T2088	punct	", ",enucleated
R1407	T2092	T2088	nsubjpass	eyes,enucleated
R1408	T2093	T2088	auxpass	were,enucleated
R1409	T2094	T2088	punct	.,enucleated
R141	T286	T276	prep	by,examined
R1410	T2096	T2097	det	A,blade
R1411	T2097	T2099	nsubjpass	blade,used
R1412	T2098	T2097	compound	scalpel,blade
R1413	T2100	T2099	auxpass	was,used
R1414	T2101	T2099	advmod	then,used
R1415	T2102	T2103	aux	to,make
R1416	T2103	T2099	advcl	make,used
R1417	T2104	T2105	det	a,incision
R1418	T2105	T2103	dobj	incision,make
R1419	T2106	T2105	amod	small,incision
R142	T287	T288	amod	standard,microscopy
R1420	T2107	T2105	prep	into,incision
R1421	T2108	T2109	det	the,chamber
R1422	T2109	T2107	pobj	chamber,into
R1423	T2110	T2109	amod	anterior,chamber
R1424	T2111	T2109	prep	near,chamber
R1425	T2112	T2113	det	the,equator
R1426	T2113	T2111	pobj	equator,near
R1427	T2114	T2099	cc	and,used
R1428	T2115	T2116	advmod	then,immersed
R1429	T2116	T2099	conj	immersed,used
R143	T288	T291	nmod	microscopy,methodologies
R1430	T2117	T2118	det	both,eyes
R1431	T2118	T2116	nsubjpass	eyes,immersed
R1432	T2119	T2118	prep	from,eyes
R1433	T2120	T2121	amod	individual,animals
R1434	T2121	T2119	pobj	animals,from
R1435	T2122	T2116	auxpass	were,immersed
R1436	T2123	T2116	prep	in,immersed
R1437	T2124	T2125	nummod	5,ml
R1438	T2125	T2126	nmod	ml,paraformaldehyde
R1439	T2126	T2123	pobj	paraformaldehyde,in
R144	T289	T288	nmod	scanning,microscopy
R1440	T2127	T2125	amod	fixative,ml
R1441	T2128	T2125	punct	(,ml
R1442	T2129	T2130	nummod	2,%
R1443	T2130	T2125	appos	%,ml
R1444	T2131	T2132	punct	(,w
R1445	T2132	T2130	parataxis	w,%
R1446	T2133	T2134	punct	/,v
R1447	T2134	T2132	prep	v,w
R1448	T2135	T2126	punct	),paraformaldehyde
R1449	T2136	T2126	cc	and,paraformaldehyde
R145	T290	T288	nmod	electron,microscopy
R1450	T2137	T2138	nummod	2,%
R1451	T2138	T2139	compound	%,glutaraldehyde
R1452	T2139	T2126	conj	glutaraldehyde,paraformaldehyde
R1453	T2140	T2141	punct	(,v
R1454	T2141	T2139	parataxis	v,glutaraldehyde
R1455	T2142	T2143	punct	/,v
R1456	T2143	T2141	prep	v,v
R1457	T2144	T2141	punct	),v
R1458	T2145	T2126	prep	in,paraformaldehyde
R1459	T2146	T2147	nummod	0.1,M
R146	T291	T286	pobj	methodologies,by
R1460	T2147	T2148	compound	M,buffer
R1461	T2148	T2145	pobj	buffer,in
R1462	T2149	T2150	compound	sodium,cacodylate
R1463	T2150	T2148	compound	cacodylate,buffer
R1464	T2151	T2126	punct	", ",paraformaldehyde
R1465	T2152	T2126	npadvmod	pH,paraformaldehyde
R1466	T2153	T2152	nummod	7.2,pH
R1467	T2154	T2116	punct	),immersed
R1468	T2155	T2116	punct	.,immersed
R1469	T2157	T2158	nsubjpass	Eyes,fixed
R147	T292	T288	cc	and,microscopy
R1470	T2159	T2158	auxpass	were,fixed
R1471	T2160	T2158	prep	for,fixed
R1472	T2161	T2162	advmod	at,24
R1473	T2162	T2164	nummod	24,hr
R1474	T2163	T2162	advmod	least,24
R1475	T2164	T2160	pobj	hr,for
R1476	T2165	T2158	prep	at,fixed
R1477	T2166	T2167	compound	room,temperature
R1478	T2167	T2165	pobj	temperature,at
R1479	T2168	T2167	punct	(,temperature
R148	T293	T294	amod	confocal,microscopy
R1480	T2169	T2167	appos	RT,temperature
R1481	T2170	T2158	punct	),fixed
R1482	T2171	T2172	amod	prior,to
R1483	T2172	T2158	prep	to,fixed
R1484	T2173	T2172	pobj	dissection,to
R1485	T2174	T2173	prep	of,dissection
R1486	T2175	T2176	det	the,lenses
R1487	T2176	T2174	pobj	lenses,of
R1488	T2177	T2158	punct	.,fixed
R1489	T2179	T2180	prep	At,recorded
R149	T294	T288	conj	microscopy,microscopy
R1490	T2181	T2182	det	this,time
R1491	T2182	T2179	pobj	time,At
R1492	T2183	T2180	punct	", ",recorded
R1493	T2184	T2185	amod	equatorial,dimensions
R1494	T2185	T2180	nsubjpass	dimensions,recorded
R1495	T2186	T2184	cc	and,equatorial
R1496	T2187	T2184	conj	axial,equatorial
R1497	T2188	T2185	prep	of,dimensions
R1498	T2189	T2188	pobj	lenses,of
R1499	T2190	T2185	cc	and,dimensions
R15	T147	T143	conj	AlphaB,AlphaA
R150	T295	T276	punct	.,examined
R1500	T2191	T2192	amod	gross,appearance
R1501	T2192	T2185	conj	appearance,dimensions
R1502	T2193	T2192	amod	lenticular,appearance
R1503	T2194	T2180	auxpass	were,recorded
R1504	T2195	T2180	punct	.,recorded
R1505	T2197	T2198	amod	Statistical,analyses
R1506	T2198	T2199	nsubj	analyses,consisted
R1507	T2200	T2198	prep	on,analyses
R1508	T2201	T2200	pobj	data,on
R1509	T2202	T2199	prep	of,consisted
R151	T299	T300	amod	Equatorial,dimensions
R1510	T2203	T2204	poss	Student,test
R1511	T2204	T2202	pobj	test,of
R1512	T2205	T2203	case	's,Student
R1513	T2206	T2204	compound	t,test
R1514	T2207	T2204	punct	-,test
R1515	T2208	T2204	punct	", ",test
R1516	T2209	T2204	conj	means,test
R1517	T2210	T2209	cc	and,means
R1518	T2211	T2212	amod	standard,deviation
R1519	T2212	T2209	conj	deviation,means
R152	T300	T306	nsubj	dimensions,were
R1520	T2213	T2199	punct	", ",consisted
R1521	T2214	T2199	advcl	using,consisted
R1522	T2215	T2216	det	the,package
R1523	T2216	T2214	dobj	package,using
R1524	T2217	T2216	amod	statistical,package
R1525	T2218	T2216	compound	software,package
R1526	T2219	T2216	appos	StatMost,package
R1527	T2220	T2221	punct	(,Angeles
R1528	T2221	T2199	parataxis	Angeles,consisted
R1529	T2222	T2223	compound	Dataxiom,Inc.
R153	T301	T299	cc	and,Equatorial
R1530	T2223	T2221	dep	Inc.,Angeles
R1531	T2224	T2223	compound	Software,Inc.
R1532	T2225	T2221	punct	", ",Angeles
R1533	T2226	T2221	compound	Los,Angeles
R1534	T2227	T2221	punct	", ",Angeles
R1535	T2228	T2221	npadvmod	CA,Angeles
R1536	T2229	T2221	punct	),Angeles
R1537	T2230	T2199	punct	.,consisted
R1538	T2289	T2290	amod	Confocal,microscopy
R1539	T2292	T2293	nsubjpass	Lenses,sectioned
R154	T302	T303	amod	axial,sagittal
R1540	T2294	T2293	auxpass	were,sectioned
R1541	T2295	T2293	dep	vibratome,sectioned
R1542	T2296	T2293	prep	into,sectioned
R1543	T2297	T2298	quantmod	100,200
R1544	T2298	T2300	nummod	200,μm
R1545	T2299	T2298	punct	–,200
R1546	T2300	T2301	npadvmod	μm,thick
R1547	T2301	T2302	amod	thick,sections
R1548	T2302	T2296	pobj	sections,into
R1549	T2303	T2293	prep	along,sectioned
R155	T303	T299	conj	sagittal,Equatorial
R1550	T2304	T2305	det	the,axes
R1551	T2305	T2303	pobj	axes,along
R1552	T2306	T2305	amod	optical,axes
R1553	T2307	T2308	punct	(,anterior
R1554	T2308	T2306	parataxis	anterior,optical
R1555	T2309	T2308	prep	to,anterior
R1556	T2310	T2309	amod	posterior,to
R1557	T2311	T2308	punct	),anterior
R1558	T2312	T2306	cc	or,optical
R1559	T2313	T2306	conj	equatorial,optical
R156	T304	T303	punct	(,sagittal
R1560	T2314	T2293	punct	.,sectioned
R1561	T2316	T2317	amod	Thick,sections
R1562	T2317	T2318	nsubjpass	sections,fixed
R1563	T2319	T2318	auxpass	were,fixed
R1564	T2320	T2318	prep	in,fixed
R1565	T2321	T2322	nummod	2,%
R1566	T2322	T2323	nmod	%,paraformaldehyde
R1567	T2323	T2320	pobj	paraformaldehyde,in
R1568	T2324	T2325	punct	(,w
R1569	T2325	T2322	parataxis	w,%
R157	T305	T303	punct	),sagittal
R1570	T2326	T2327	punct	/,v
R1571	T2327	T2325	prep	v,w
R1572	T2328	T2325	punct	),w
R1573	T2329	T2323	cc	and,paraformaldehyde
R1574	T2330	T2331	nummod	2,%
R1575	T2331	T2332	compound	%,glutaraldehyde
R1576	T2332	T2323	conj	glutaraldehyde,paraformaldehyde
R1577	T2333	T2334	punct	(,v
R1578	T2334	T2332	parataxis	v,glutaraldehyde
R1579	T2335	T2336	punct	/,v
R158	T307	T300	prep	of,dimensions
R1580	T2336	T2334	prep	v,v
R1581	T2337	T2334	punct	),v
R1582	T2338	T2323	prep	in,paraformaldehyde
R1583	T2339	T2340	nummod	0.1,M
R1584	T2340	T2341	compound	M,buffer
R1585	T2341	T2338	pobj	buffer,in
R1586	T2342	T2343	compound	sodium,cacodylate
R1587	T2343	T2341	compound	cacodylate,buffer
R1588	T2344	T2323	npadvmod	pH,paraformaldehyde
R1589	T2345	T2344	nummod	7.2,pH
R159	T308	T307	pobj	lenses,of
R1590	T2346	T2318	prep	at,fixed
R1591	T2347	T2346	pobj	RT,at
R1592	T2348	T2318	prep	for,fixed
R1593	T2349	T2350	nummod	24,hrs
R1594	T2350	T2348	pobj	hrs,for
R1595	T2351	T2352	advmod	then,washed
R1596	T2352	T2318	dep	washed,fixed
R1597	T2353	T2354	amod	several,times
R1598	T2354	T2352	npadvmod	times,washed
R1599	T2355	T2352	prep	in,washed
R16	T148	T144	punct	-,crystallin
R160	T309	T308	prep	for,lenses
R1600	T2356	T2357	npadvmod	Tris,buffered
R1601	T2357	T2358	amod	buffered,saline
R1602	T2358	T2355	pobj	saline,in
R1603	T2359	T2360	punct	(,pH
R1604	T2360	T2358	parataxis	pH,saline
R1605	T2361	T2362	nummod	0.5,M
R1606	T2362	T2363	compound	M,Tris
R1607	T2363	T2360	dep	Tris,pH
R1608	T2364	T2360	punct	", ",pH
R1609	T2365	T2366	nummod	150,mM
R161	T310	T311	compound	alphaA,BKO
R1610	T2366	T2367	compound	mM,NaCl
R1611	T2367	T2360	dep	NaCl,pH
R1612	T2368	T2360	punct	", ",pH
R1613	T2369	T2360	nummod	7.4,pH
R1614	T2370	T2360	punct	),pH
R1615	T2371	T2318	punct	.,fixed
R1616	T2373	T2374	aux	To,visualize
R1617	T2374	T2375	advcl	visualize,stained
R1618	T2376	T2377	compound	lipid,membranes
R1619	T2377	T2374	dobj	membranes,visualize
R162	T311	T313	compound	BKO,mice
R1620	T2378	T2375	punct	", ",stained
R1621	T2379	T2375	nsubjpass	sections,stained
R1622	T2380	T2375	auxpass	were,stained
R1623	T2381	T2375	prep	with,stained
R1624	T2382	T2383	nummod	1,dioctadecyl
R1625	T2383	T2381	pobj	dioctadecyl,with
R1626	T2384	T2382	punct	",",1
R1627	T2385	T2382	appos	1,1
R1628	T2386	T2385	punct	',1
R1629	T2387	T2383	punct	-,dioctadecyl
R163	T312	T311	punct	/,BKO
R1630	T2388	T2383	punct	-,dioctadecyl
R1631	T2389	T2383	nummod	3,dioctadecyl
R1632	T2390	T2383	punct	",",dioctadecyl
R1633	T2391	T2392	nummod	3,perchlorate
R1634	T2392	T2383	appos	perchlorate,dioctadecyl
R1635	T2393	T2391	punct	",",3
R1636	T2394	T2391	appos	3,3
R1637	T2395	T2394	punct	',3
R1638	T2396	T2391	punct	",",3
R1639	T2397	T2391	appos	3,3
R164	T313	T309	pobj	mice,for
R1640	T2398	T2397	punct	',3
R1641	T2399	T2391	punct	-,3
R1642	T2400	T2391	amod	tetramethylindocarbocyanine,3
R1643	T2401	T2402	punct	(,Probes
R1644	T2402	T2383	parataxis	Probes,dioctadecyl
R1645	T2403	T2402	punct	',Probes
R1646	T2404	T2402	dep	DiI,Probes
R1647	T2405	T2402	punct	',Probes
R1648	T2406	T2402	punct	;,Probes
R1649	T2407	T2402	dep	DiIC18,Probes
R165	T314	T315	advmod	significantly,smaller
R1650	T2408	T2402	punct	;,Probes
R1651	T2409	T2402	compound	Molecular,Probes
R1652	T2410	T2402	punct	),Probes
R1653	T2411	T2412	mark	as,described
R1654	T2412	T2375	advcl	described,stained
R1655	T2413	T2412	advmod	previously,described
R1656	T2414	T2415	punct	[,24
R1657	T2415	T2375	parataxis	24,stained
R1658	T2416	T2415	punct	],24
R1659	T2417	T2375	punct	.,stained
R166	T315	T306	acomp	smaller,were
R1660	T2419	T2420	nsubjpass	Sections,washed
R1661	T2421	T2420	auxpass	were,washed
R1662	T2422	T2420	advmod	then,washed
R1663	T2423	T2424	amod	several,times
R1664	T2424	T2420	npadvmod	times,washed
R1665	T2425	T2420	prep	with,washed
R1666	T2426	T2427	npadvmod	Tris,buffered
R1667	T2427	T2428	amod	buffered,saline
R1668	T2428	T2425	pobj	saline,with
R1669	T2429	T2420	punct	", ",washed
R167	T316	T315	prep	than,smaller
R1670	T2430	T2420	cc	and,washed
R1671	T2431	T2432	amod	nucleic,acids
R1672	T2432	T2433	nsubjpass	acids,stained
R1673	T2433	T2420	conj	stained,washed
R1674	T2434	T2433	auxpass	were,stained
R1675	T2435	T2433	prep	by,stained
R1676	T2436	T2435	pcomp	incubating,by
R1677	T2437	T2436	dobj	sections,incubating
R1678	T2438	T2436	prep	in,incubating
R1679	T2439	T2438	pobj	TBS,in
R168	T317	T318	npadvmod	age,matched
R1680	T2440	T2439	acl	containing,TBS
R1681	T2441	T2442	nummod	1,μM
R1682	T2442	T2443	compound	μM,Green
R1683	T2443	T2440	dobj	Green,containing
R1684	T2444	T2443	compound	SYTOX,Green
R1685	T2445	T2446	punct	(,Probes
R1686	T2446	T2440	parataxis	Probes,containing
R1687	T2447	T2446	amod	Molecular,Probes
R1688	T2448	T2446	punct	),Probes
R1689	T2449	T2436	prep	for,incubating
R169	T318	T320	amod	matched,lenses
R1690	T2450	T2451	nummod	10,min
R1691	T2451	T2449	pobj	min,for
R1692	T2452	T2436	prep	at,incubating
R1693	T2453	T2452	pobj	RT,at
R1694	T2454	T2436	advcl	followed,incubating
R1695	T2455	T2454	agent	by,followed
R1696	T2456	T2457	amod	several,washes
R1697	T2457	T2455	pobj	washes,by
R1698	T2458	T2457	prep	with,washes
R1699	T2459	T2458	pobj	TBS,with
R17	T149	T150	amod	double,knockout
R170	T319	T318	punct	-,matched
R1700	T2460	T2457	advmod	prior,washes
R1701	T2461	T2460	prep	to,prior
R1702	T2462	T2463	amod	confocal,imaging
R1703	T2463	T2461	pobj	imaging,to
R1704	T2464	T2433	punct	.,stained
R1705	T2466	T2467	compound	Lens,sections
R1706	T2467	T2468	nsubjpass	sections,viewed
R1707	T2469	T2468	auxpass	were,viewed
R1708	T2470	T2468	prep	on,viewed
R1709	T2471	T2472	det	a,microscope
R171	T320	T316	pobj	lenses,than
R1710	T2472	T2470	pobj	microscope,on
R1711	T2473	T2474	nmod	Zeiss,laser
R1712	T2474	T2472	nmod	laser,microscope
R1713	T2475	T2472	nmod	scanning,microscope
R1714	T2476	T2472	amod	confocal,microscope
R1715	T2477	T2478	compound	model,LSM
R1716	T2478	T2472	appos	LSM,microscope
R1717	T2479	T2478	nummod	410,LSM
R1718	T2480	T2472	acl	equipped,microscope
R1719	T2481	T2480	prep	with,equipped
R172	T321	T322	amod	wild,type
R1720	T2482	T2483	det	an,microscope
R1721	T2483	T2481	pobj	microscope,with
R1722	T2484	T2485	amod	Axiovert,100
R1723	T2485	T2483	nummod	100,microscope
R1724	T2486	T2483	amod	inverted,microscope
R1725	T2487	T2483	punct	", ",microscope
R1726	T2488	T2489	det	an,laser
R1727	T2489	T2483	conj	laser,microscope
R1728	T2490	T2491	amod	Argon,Krypton
R1729	T2491	T2489	nmod	Krypton,laser
R173	T322	T320	compound	type,lenses
R1730	T2492	T2491	punct	-,Krypton
R1731	T2493	T2491	nummod	488,Krypton
R1732	T2494	T2491	punct	/,Krypton
R1733	T2495	T2491	nummod	568,Krypton
R1734	T2496	T2491	punct	/,Krypton
R1735	T2497	T2491	nummod	647,Krypton
R1736	T2498	T2489	punct	", ",laser
R1737	T2499	T2500	det	a,filter
R1738	T2500	T2489	conj	filter,laser
R1739	T2501	T2500	compound	KP,filter
R174	T323	T306	punct	.,were
R1740	T2502	T2503	compound	line,selection
R1741	T2503	T2500	compound	selection,filter
R1742	T2504	T2500	punct	", ",filter
R1743	T2505	T2506	det	a,splitter
R1744	T2506	T2500	conj	splitter,filter
R1745	T2507	T2506	nmod	FT,splitter
R1746	T2508	T2507	nummod	488,FT
R1747	T2509	T2507	punct	/,FT
R1748	T2510	T2507	nummod	568,FT
R1749	T2511	T2506	amod	Dichroic,splitter
R175	T325	T326	det	No,sutures
R1750	T2512	T2506	compound	beam,splitter
R1751	T2513	T2506	punct	", ",splitter
R1752	T2514	T2515	det	a,splitter
R1753	T2515	T2506	conj	splitter,splitter
R1754	T2516	T2515	nmod	FT,splitter
R1755	T2517	T2516	nummod	560,FT
R1756	T2518	T2515	amod	Dichroic,splitter
R1757	T2519	T2515	compound	beam,splitter
R1758	T2520	T2515	punct	", ",splitter
R1759	T2521	T2522	det	a,filter
R176	T326	T328	nsubjpass	sutures,found
R1760	T2522	T2515	conj	filter,splitter
R1761	T2523	T2522	nmod	LP,filter
R1762	T2524	T2523	nummod	590,LP
R1763	T2525	T2522	compound	emission,filter
R1764	T2526	T2522	prep	for,filter
R1765	T2527	T2526	pcomp	viewing,for
R1766	T2528	T2527	dobj	DiI,viewing
R1767	T2529	T2522	punct	", ",filter
R1768	T2530	T2531	det	a,filter
R1769	T2531	T2522	conj	filter,filter
R177	T327	T326	amod	posterior,sutures
R1770	T2532	T2531	nmod	BP,filter
R1771	T2533	T2532	nummod	515,BP
R1772	T2534	T2535	punct	–,540
R1773	T2535	T2532	prep	540,BP
R1774	T2536	T2531	compound	emission,filter
R1775	T2537	T2531	prep	for,filter
R1776	T2538	T2537	pcomp	viewing,for
R1777	T2539	T2540	compound	SYTOX,green
R1778	T2540	T2538	dobj	green,viewing
R1779	T2541	T2531	punct	", ",filter
R178	T329	T326	cc	or,sutures
R1780	T2542	T2531	cc	and,filter
R1781	T2543	T2544	det	the,package
R1782	T2544	T2531	conj	package,filter
R1783	T2545	T2544	compound	software,package
R1784	T2546	T2544	appos	LSM,package
R1785	T2547	T2546	nummod	3.993,LSM
R1786	T2548	T2468	punct	.,viewed
R1787	T2588	T2589	compound	Scanning,microscopy
R1788	T2590	T2589	compound	electron,microscopy
R1789	T2592	T2593	nsubjpass	Lenses,fixed
R179	T330	T331	compound	fiber,cells
R1790	T2594	T2592	acl	bisected,Lenses
R1791	T2595	T2594	prep	along,bisected
R1792	T2596	T2597	det	the,axes
R1793	T2597	T2595	pobj	axes,along
R1794	T2598	T2597	amod	optical,axes
R1795	T2599	T2593	auxpass	were,fixed
R1796	T2600	T2593	dep	immersion,fixed
R1797	T2601	T2593	prep	in,fixed
R1798	T2602	T2603	nummod	2,%
R1799	T2603	T2604	nmod	%,paraformaldehyde
R18	T150	T142	compound	knockout,lens
R180	T331	T326	conj	cells,sutures
R1800	T2604	T2601	pobj	paraformaldehyde,in
R1801	T2605	T2606	punct	(,w
R1802	T2606	T2603	parataxis	w,%
R1803	T2607	T2608	punct	/,v
R1804	T2608	T2606	prep	v,w
R1805	T2609	T2606	punct	),w
R1806	T2610	T2604	cc	and,paraformaldehyde
R1807	T2611	T2612	nummod	2,%
R1808	T2612	T2613	compound	%,glutaraldehyde
R1809	T2613	T2604	conj	glutaraldehyde,paraformaldehyde
R181	T332	T326	acl	extending,sutures
R1810	T2614	T2615	punct	(,v
R1811	T2615	T2613	parataxis	v,glutaraldehyde
R1812	T2616	T2617	punct	/,v
R1813	T2617	T2615	prep	v,v
R1814	T2618	T2615	punct	),v
R1815	T2619	T2604	prep	in,paraformaldehyde
R1816	T2620	T2621	nummod	0.1,M
R1817	T2621	T2622	compound	M,cacodylate
R1818	T2622	T2624	compound	cacodylate,buffer
R1819	T2623	T2622	compound	sodium,cacodylate
R182	T333	T332	prep	to,extending
R1820	T2624	T2619	pobj	buffer,in
R1821	T2625	T2624	npadvmod	pH,buffer
R1822	T2626	T2625	nummod	7.2,pH
R1823	T2627	T2593	prep	at,fixed
R1824	T2628	T2627	pobj	RT,at
R1825	T2629	T2593	prep	for,fixed
R1826	T2630	T2631	nummod	24,hrs
R1827	T2631	T2629	pobj	hrs,for
R1828	T2632	T2593	punct	.,fixed
R1829	T2634	T2635	nsubjpass	Samples,washed
R183	T334	T335	det	the,capsule
R1830	T2636	T2635	auxpass	were,washed
R1831	T2637	T2635	advmod	then,washed
R1832	T2638	T2639	amod	several,times
R1833	T2639	T2635	npadvmod	times,washed
R1834	T2640	T2635	prep	with,washed
R1835	T2641	T2642	amod	distilled,water
R1836	T2642	T2640	pobj	water,with
R1837	T2643	T2635	cc	and,washed
R1838	T2644	T2635	conj	postfixed,washed
R1839	T2645	T2644	prep	in,postfixed
R184	T335	T333	pobj	capsule,to
R1840	T2646	T2647	nummod	1,%
R1841	T2647	T2648	nmod	%,tetroxide
R1842	T2648	T2645	pobj	tetroxide,in
R1843	T2649	T2650	punct	(,v
R1844	T2650	T2648	parataxis	v,tetroxide
R1845	T2651	T2652	punct	/,v
R1846	T2652	T2650	prep	v,v
R1847	T2653	T2650	punct	),v
R1848	T2654	T2648	amod	aqueous,tetroxide
R1849	T2655	T2648	compound	osmium,tetroxide
R185	T336	T335	amod	posterior,capsule
R1850	T2656	T2644	prep	at,postfixed
R1851	T2657	T2656	pobj	RT,at
R1852	T2658	T2644	prep	for,postfixed
R1853	T2659	T2660	nummod	1,hr
R1854	T2660	T2658	pobj	hr,for
R1855	T2661	T2635	punct	.,washed
R1856	T2663	T2664	compound	Lens,halves
R1857	T2664	T2665	nsubjpass	halves,dehydrated
R1858	T2666	T2665	auxpass	were,dehydrated
R1859	T2667	T2665	prep	in,dehydrated
R186	T337	T335	prep	of,capsule
R1860	T2668	T2669	det	an,series
R1861	T2669	T2667	pobj	series,in
R1862	T2670	T2669	amod	ascending,series
R1863	T2671	T2669	compound	ethanol,series
R1864	T2672	T2669	prep	from,series
R1865	T2673	T2674	quantmod	50,100
R1866	T2674	T2676	nummod	100,%
R1867	T2675	T2674	punct	–,100
R1868	T2676	T2672	pobj	%,from
R1869	T2677	T2665	punct	.,dehydrated
R187	T338	T339	det	the,lens
R1870	T2679	T2680	mark	Once,were
R1871	T2680	T2683	advcl	were,dried
R1872	T2681	T2682	compound	lens,halves
R1873	T2682	T2680	nsubj	halves,were
R1874	T2684	T2680	prep	in,were
R1875	T2685	T2686	nummod	100,%
R1876	T2686	T2687	compound	%,ethanol
R1877	T2687	T2684	pobj	ethanol,in
R1878	T2688	T2683	punct	", ",dried
R1879	T2689	T2683	nsubjpass	they,dried
R188	T339	T337	pobj	lens,of
R1880	T2690	T2683	auxpass	were,dried
R1881	T2691	T2692	amod	critical,point
R1882	T2692	T2683	npadvmod	point,dried
R1883	T2693	T2683	prep	in,dried
R1884	T2694	T2695	compound	carbon,dioxide
R1885	T2695	T2693	pobj	dioxide,in
R1886	T2696	T2683	prep	in,dried
R1887	T2697	T2698	det	a,790B
R1888	T2698	T2696	pobj	790B,in
R1889	T2699	T2698	compound	Samdri,790B
R189	T340	T328	auxpass	were,found
R1890	T2700	T2698	punct	-,790B
R1891	T2701	T2702	punct	(,Corp.
R1892	T2702	T2698	parataxis	Corp.,790B
R1893	T2703	T2702	compound	Tousimis,Corp.
R1894	T2704	T2702	compound	Research,Corp.
R1895	T2705	T2702	punct	", ",Corp.
R1896	T2706	T2702	npadvmod	Rockville,Corp.
R1897	T2707	T2702	punct	", ",Corp.
R1898	T2708	T2702	npadvmod	MD,Corp.
R1899	T2709	T2702	punct	),Corp.
R19	T151	T142	compound	mouse,lens
R190	T341	T328	prep	in,found
R1900	T2710	T2683	punct	.,dried
R1901	T2712	T2713	amod	Critical,point
R1902	T2713	T2714	npadvmod	point,dried
R1903	T2714	T2715	amod	dried,halves
R1904	T2715	T2717	nsubjpass	halves,secured
R1905	T2716	T2715	compound	lens,halves
R1906	T2718	T2717	auxpass	were,secured
R1907	T2719	T2717	prep	on,secured
R1908	T2720	T2721	compound	aluminum,stubs
R1909	T2721	T2719	pobj	stubs,on
R191	T342	T343	compound	alphaA,BKO
R1910	T2722	T2717	prep	with,secured
R1911	T2723	T2724	npadvmod	double,sided
R1912	T2724	T2725	amod	sided,tape
R1913	T2725	T2722	pobj	tape,with
R1914	T2726	T2717	punct	.,secured
R1915	T2728	T2729	amod	Mounted,specimens
R1916	T2729	T2730	nsubjpass	specimens,coated
R1917	T2731	T2730	auxpass	were,coated
R1918	T2732	T2730	advmod	then,coated
R1919	T2733	T2730	dep	sputter,coated
R192	T343	T345	compound	BKO,lenses
R1920	T2734	T2730	prep	with,coated
R1921	T2735	T2734	pobj	gold,with
R1922	T2736	T2730	cc	and,coated
R1923	T2737	T2730	conj	viewed,coated
R1924	T2738	T2737	prep	on,viewed
R1925	T2739	T2740	det	a,microscope
R1926	T2740	T2738	pobj	microscope,on
R1927	T2741	T2742	compound	Hitachi,3500N
R1928	T2742	T2740	compound	3500N,microscope
R1929	T2743	T2742	compound	S,3500N
R193	T344	T343	punct	/,BKO
R1930	T2744	T2742	punct	-,3500N
R1931	T2745	T2740	compound	scanning,microscope
R1932	T2746	T2740	compound	electron,microscope
R1933	T2747	T2748	punct	(,Tokyo
R1934	T2748	T2740	parataxis	Tokyo,microscope
R1935	T2749	T2748	punct	", ",Tokyo
R1936	T2750	T2748	npadvmod	Japan,Tokyo
R1937	T2751	T2748	punct	),Tokyo
R1938	T2752	T2737	prep	at,viewed
R1939	T2753	T2754	quantmod	1,5
R194	T345	T341	pobj	lenses,in
R1940	T2754	T2756	nummod	5,kV
R1941	T2755	T2754	punct	–,5
R1942	T2756	T2752	pobj	kV,at
R1943	T2757	T2730	punct	.,coated
R1944	T2759	T2760	det	This,microscope
R1945	T2760	T2761	nsubjpass	microscope,equipped
R1946	T2762	T2761	auxpass	is,equipped
R1947	T2763	T2761	prep	with,equipped
R1948	T2764	T2765	poss	Hitachi,Bias
R1949	T2765	T2763	pobj	Bias,with
R195	T346	T328	punct	.,found
R1950	T2766	T2764	case	's,Hitachi
R1951	T2767	T2765	amod	patented,Bias
R1952	T2768	T2765	punct	"""",Bias
R1953	T2769	T2765	compound	Dual,Bias
R1954	T2770	T2765	punct	-,Bias
R1955	T2771	T2765	punct	"""",Bias
R1956	T2772	T2773	dep	which,allows
R1957	T2773	T2765	relcl	allows,Bias
R1958	T2774	T2775	advmod	extremely,high
R1959	T2775	T2776	amod	high,currents
R196	T348	T349	amod	Ectopical,staining
R1960	T2776	T2773	dobj	currents,allows
R1961	T2777	T2776	compound	emission,currents
R1962	T2778	T2773	prep	at,allows
R1963	T2779	T2780	compound	acceleration,voltages
R1964	T2780	T2778	pobj	voltages,at
R1965	T2781	T2780	prep	of,voltages
R1966	T2782	T2783	nummod	5,kV
R1967	T2783	T2781	pobj	kV,of
R1968	T2784	T2783	cc	and,kV
R1969	T2785	T2783	conj	lower,kV
R197	T349	T352	nsubjpass	staining,observed
R1970	T2786	T2761	punct	.,equipped
R1977	T3289	T3290	amod	Equatorial,dimensions
R1978	T3290	T3296	nsubjpass	dimensions,recorded
R1979	T3291	T3289	cc	and,Equatorial
R198	T350	T351	amod	nucleic,acid
R1980	T3292	T3293	amod	axial,sagittal
R1981	T3293	T3289	conj	sagittal,Equatorial
R1982	T3294	T3293	punct	(,sagittal
R1983	T3295	T3290	punct	),dimensions
R1984	T3297	T3290	prep	of,dimensions
R1985	T3298	T3297	pobj	lenses,of
R1986	T3299	T3290	punct	", ",dimensions
R1987	T3300	T3290	cc	and,dimensions
R1988	T3301	T3302	amod	gross,appearance
R1989	T3302	T3290	conj	appearance,dimensions
R199	T351	T349	compound	acid,staining
R1990	T3303	T3302	amod	lenticular,appearance
R1991	T3304	T3296	punct	", ",recorded
R1992	T3305	T3296	auxpass	were,recorded
R1993	T3306	T3307	punct	(,1
R1994	T3307	T3296	parataxis	1,recorded
R1995	T3308	T3307	amod	Table,1
R1996	T3309	T3307	punct	),1
R1997	T3310	T3296	punct	.,recorded
R1998	T3312	T3313	compound	AlphaA,BKO
R1999	T3313	T3315	compound	BKO,lenses
R20	T156	T157	nummod	One,approach
R200	T353	T352	auxpass	was,observed
R2000	T3314	T3313	punct	/,BKO
R2001	T3315	T3316	nsubj	lenses,were
R2002	T3317	T3315	prep	at,lenses
R2003	T3318	T3319	nummod	5,wks
R2004	T3319	T3317	pobj	wks,at
R2005	T3320	T3319	prep	of,wks
R2006	T3321	T3320	pobj	age,of
R2007	T3322	T3323	advmod	significantly,smaller
R2008	T3323	T3316	acomp	smaller,were
R2009	T3324	T3325	punct	(,0.05
R201	T354	T352	prep	in,observed
R2010	T3325	T3323	parataxis	0.05,smaller
R2011	T3326	T3325	nsubj	p,0.05
R2012	T3327	T3325	punct	<,0.05
R2013	T3328	T3325	punct	),0.05
R2014	T3329	T3316	punct	", ",were
R2015	T3330	T3331	preconj	both,equatorially
R2016	T3331	T3316	advmod	equatorially,were
R2017	T3332	T3331	cc	and,equatorially
R2018	T3333	T3331	conj	axially,equatorially
R2019	T3334	T3316	punct	", ",were
R202	T355	T356	det	the,region
R2020	T3335	T3316	prep	than,were
R2021	T3336	T3337	nummod	5,wk
R2022	T3337	T3338	npadvmod	wk,old
R2023	T3338	T3339	amod	old,lenses
R2024	T3339	T3335	pobj	lenses,than
R2025	T3340	T3341	amod	wild,type
R2026	T3341	T3339	compound	type,lenses
R2027	T3342	T3316	cc	and,were
R2028	T3343	T3316	conj	remain,were
R2029	T3344	T3343	oprd	smaller,remain
R203	T356	T354	pobj	region,in
R2030	T3345	T3343	prep	throughout,remain
R2031	T3346	T3345	pobj	life,throughout
R2032	T3347	T3316	punct	.,were
R2033	T3349	T3350	compound	Fifty,four
R2034	T3350	T3352	nummod	four,wk
R2035	T3351	T3350	punct	-,four
R2036	T3352	T3353	npadvmod	wk,old
R2037	T3353	T3354	amod	old,lenses
R2038	T3354	T3358	nsubj	lenses,were
R2039	T3355	T3356	compound	alphaA,BKO
R204	T357	T356	amod	posterior,region
R2040	T3356	T3354	compound	BKO,lenses
R2041	T3357	T3356	punct	/,BKO
R2042	T3359	T3360	advmod	much,smaller
R2043	T3360	T3358	acomp	smaller,were
R2044	T3361	T3360	prep	than,smaller
R2045	T3362	T3363	advmod	similarly,aged
R2046	T3363	T3364	amod	aged,lenses
R2047	T3364	T3361	pobj	lenses,than
R2048	T3365	T3364	amod	normal,lenses
R2049	T3366	T3358	punct	", ",were
R205	T358	T356	amod	subcapsular,region
R2050	T3367	T3358	cc	and,were
R2051	T3368	T3358	conj	were,were
R2052	T3369	T3368	acomp	similar,were
R2053	T3370	T3369	prep	in,similar
R2054	T3371	T3370	pobj	size,in
R2055	T3372	T3369	prep	to,similar
R2056	T3373	T3374	nummod	5,wk
R2057	T3374	T3375	npadvmod	wk,old
R2058	T3375	T3376	amod	old,lenses
R2059	T3376	T3372	pobj	lenses,to
R206	T359	T356	prep	of,region
R2060	T3377	T3378	amod	wild,type
R2061	T3378	T3376	compound	type,lenses
R2062	T3379	T3358	punct	.,were
R2063	T3381	T3382	preconj	Both,lenses
R2064	T3382	T3389	nsubj	lenses,were
R2065	T3383	T3382	det	the,lenses
R2066	T3384	T3385	nummod	5,wk
R2067	T3385	T3386	npadvmod	wk,old
R2068	T3386	T3382	amod	old,lenses
R2069	T3387	T3388	amod	wild,type
R207	T360	T361	nummod	5,wk
R2070	T3388	T3382	compound	type,lenses
R2071	T3390	T3382	cc	and,lenses
R2072	T3391	T3392	det	the,lenses
R2073	T3392	T3382	conj	lenses,lenses
R2074	T3393	T3394	nummod	54,wk
R2075	T3394	T3395	npadvmod	wk,old
R2076	T3395	T3392	amod	old,lenses
R2077	T3396	T3397	compound	alphaA,BKO
R2078	T3397	T3392	compound	BKO,lenses
R2079	T3398	T3397	punct	/,BKO
R208	T361	T359	pobj	wk,of
R2080	T3399	T3400	advmod	significantly,smaller
R2081	T3400	T3389	acomp	smaller,were
R2082	T3401	T3402	punct	(,0.01
R2083	T3402	T3400	parataxis	0.01,smaller
R2084	T3403	T3402	nsubj	p,0.01
R2085	T3404	T3402	punct	<,0.01
R2086	T3405	T3402	punct	),0.01
R2087	T3406	T3400	prep	than,smaller
R2088	T3407	T3408	det	the,lenses
R2089	T3408	T3406	pobj	lenses,than
R209	T362	T356	cc	and,region
R2090	T3409	T3410	nummod	46,wk
R2091	T3410	T3413	npadvmod	wk,old
R2092	T3411	T3409	cc	or,46
R2093	T3412	T3409	conj	72,46
R2094	T3413	T3408	amod	old,lenses
R2095	T3414	T3415	amod	wild,type
R2096	T3415	T3408	compound	type,lenses
R2097	T3416	T3389	punct	.,were
R2098	T3418	T3419	compound	AlphaA,BKO
R2099	T3419	T3421	compound	BKO,lenses
R21	T157	T158	nsubj	approach,is
R210	T363	T364	amod	anterior,cortex
R2100	T3420	T3419	punct	/,BKO
R2101	T3421	T3422	nsubj	lenses,exhibited
R2102	T3423	T3421	prep	at,lenses
R2103	T3424	T3425	nummod	5,wks
R2104	T3425	T3423	pobj	wks,at
R2105	T3426	T3425	prep	of,wks
R2106	T3427	T3426	pobj	age,of
R2107	T3428	T3422	dobj	vacuoles,exhibited
R2108	T3429	T3428	prep	at,vacuoles
R2109	T3430	T3431	det	the,region
R211	T364	T366	nmod	cortex,lenses
R2110	T3431	T3429	pobj	region,at
R2111	T3432	T3431	amod	equatorial,region
R2112	T3433	T3434	punct	(,1A
R2113	T3434	T3428	parataxis	1A,vacuoles
R2114	T3435	T3434	compound	Figure,1A
R2115	T3436	T3434	advcl	see,1A
R2116	T3437	T3436	dobj	arrows,see
R2117	T3438	T3434	punct	),1A
R2118	T3439	T3428	cc	and,vacuoles
R2119	T3440	T3441	det	an,area
R212	T365	T364	amod	subcapsular,cortex
R2120	T3441	T3428	conj	area,vacuoles
R2121	T3442	T3441	prep	of,area
R2122	T3443	T3444	amod	slight,scatter
R2123	T3444	T3442	pobj	scatter,of
R2124	T3445	T3444	amod	light,scatter
R2125	T3446	T3441	prep	at,area
R2126	T3447	T3448	det	the,regions
R2127	T3448	T3446	pobj	regions,at
R2128	T3449	T3448	amod	posterior,regions
R2129	T3450	T3448	amod	subcapsular,regions
R213	T366	T356	conj	lenses,region
R2130	T3451	T3452	punct	(,1B
R2131	T3452	T3441	parataxis	1B,area
R2132	T3453	T3452	compound	Figure,1B
R2133	T3454	T3452	advcl	see,1B
R2134	T3455	T3454	dobj	arrowheads,see
R2135	T3456	T3452	punct	),1B
R2136	T3457	T3422	punct	.,exhibited
R2137	T3459	T3460	prep	By,exhibited
R2138	T3461	T3462	nummod	54,wks
R2139	T3462	T3459	pobj	wks,By
R214	T367	T364	prep	of,cortex
R2140	T3463	T3462	prep	of,wks
R2141	T3464	T3463	pobj	age,of
R2142	T3465	T3460	punct	", ",exhibited
R2143	T3466	T3467	compound	alphaA,BKO
R2144	T3467	T3469	compound	BKO,lenses
R2145	T3468	T3467	punct	/,BKO
R2146	T3469	T3460	nsubj	lenses,exhibited
R2147	T3470	T3471	amod	dense,cataracts
R2148	T3471	T3460	dobj	cataracts,exhibited
R2149	T3472	T3471	amod	cortical,cataracts
R215	T368	T369	nummod	54,wk
R2150	T3473	T3472	cc	and,cortical
R2151	T3474	T3472	conj	nuclear,cortical
R2152	T3475	T3476	punct	(,1C
R2153	T3476	T3460	parataxis	1C,exhibited
R2154	T3477	T3476	compound	Figure,1C
R2155	T3478	T3476	punct	),1C
R2156	T3479	T3460	punct	.,exhibited
R2157	T3481	T3482	det	No,sutures
R2158	T3482	T3484	nsubjpass	sutures,observed
R2159	T3483	T3482	amod	posterior,sutures
R216	T369	T367	pobj	wk,of
R2160	T3485	T3484	auxpass	were,observed
R2161	T3486	T3484	prep	in,observed
R2162	T3487	T3486	pobj	any,in
R2163	T3488	T3487	prep	of,any
R2164	T3489	T3490	det	the,lenses
R2165	T3490	T3488	pobj	lenses,of
R2166	T3491	T3492	compound	alphaA,BKO
R2167	T3492	T3490	compound	BKO,lenses
R2168	T3493	T3492	punct	/,BKO
R2169	T3494	T3490	acl	examined,lenses
R217	T370	T371	compound	alphaA,BKO
R2170	T3495	T3484	punct	.,observed
R2171	T3497	T3498	det	All,lenses
R2172	T3498	T3501	nsubj	lenses,were
R2173	T3499	T3500	amod	wild,type
R2174	T3500	T3498	compound	type,lenses
R2175	T3502	T3501	acomp	transparent,were
R2176	T3503	T3501	prep	upon,were
R2177	T3504	T3503	pobj	removal,upon
R2178	T3505	T3504	prep	from,removal
R2179	T3506	T3507	det	the,eye
R218	T371	T366	compound	BKO,lenses
R2180	T3507	T3505	pobj	eye,from
R2181	T3508	T3509	punct	(,1D
R2182	T3509	T3501	parataxis	1D,were
R2183	T3510	T3509	compound	Figure,1D
R2184	T3511	T3509	punct	",",1D
R2185	T3512	T3509	conj	1E,1D
R2186	T3513	T3512	cc	and,1E
R2187	T3514	T3512	conj	1F,1E
R2188	T3515	T3509	punct	),1D
R2189	T3516	T3501	cc	and,were
R219	T372	T371	punct	/,BKO
R2190	T3517	T3501	conj	had,were
R2191	T3518	T3519	advmod	well,defined
R2192	T3519	T3520	amod	defined,sutures
R2193	T3520	T3517	dobj	sutures,had
R2194	T3521	T3520	amod	posterior,sutures
R2195	T3522	T3501	punct	.,were
R2196	T3527	T3528	nsubj	xamination,revealed
R2197	T3529	T3527	prep	of,xamination
R2198	T3530	T3531	amod	mid-axial,sections
R2199	T3531	T3529	pobj	sections,of
R22	T159	T157	prep	to,approach
R220	T373	T352	punct	.,observed
R2200	T3532	T3531	acl	stained,sections
R2201	T3533	T3532	prep	with,stained
R2202	T3534	T3533	pobj	DiI,with
R2203	T3535	T3534	cc	and,DiI
R2204	T3536	T3537	compound	SYTOX,green
R2205	T3537	T3534	conj	green,DiI
R2206	T3538	T3539	amod	unique,differences
R2207	T3539	T3528	dobj	differences,revealed
R2208	T3540	T3539	prep	in,differences
R2209	T3541	T3542	amod	gross,morphology
R221	T375	T376	amod	Gross,differences
R2210	T3542	T3540	pobj	morphology,in
R2211	T3543	T3542	cc	and,morphology
R2212	T3544	T3545	amod	nucleic,acid
R2213	T3545	T3546	compound	acid,staining
R2214	T3546	T3542	conj	staining,morphology
R2215	T3547	T3539	prep	between,differences
R2216	T3548	T3549	compound	alphaA,BKO
R2217	T3549	T3551	compound	BKO,lenses
R2218	T3550	T3549	punct	/,BKO
R2219	T3551	T3547	pobj	lenses,between
R222	T376	T378	nsubjpass	differences,observed
R2220	T3552	T3551	cc	and,lenses
R2221	T3553	T3554	amod	wild,type
R2222	T3554	T3555	compound	type,lenses
R2223	T3555	T3551	conj	lenses,lenses
R2224	T3556	T3557	punct	(,Figure
R2225	T3557	T3528	parataxis	Figure,revealed
R2226	T3558	T3557	nummod	2,Figure
R2227	T3559	T3557	punct	),Figure
R2228	T3560	T3528	punct	.,revealed
R2229	T3562	T3563	prep	In,nuclei
R223	T377	T376	amod	morphological,differences
R2230	T3564	T3565	nummod	five,week
R2231	T3565	T3566	npadvmod	week,old
R2232	T3566	T3567	amod	old,lenses
R2233	T3567	T3562	pobj	lenses,In
R2234	T3568	T3569	compound	alphaA,BKO
R2235	T3569	T3567	compound	BKO,lenses
R2236	T3570	T3569	punct	/,BKO
R2237	T3571	T3563	punct	", ",nuclei
R2238	T3572	T3563	nmod	Sytox,nuclei
R2239	T3573	T3574	amod	green,stained
R224	T379	T378	auxpass	were,observed
R2240	T3574	T3563	amod	stained,nuclei
R2241	T3575	T3576	punct	(,green
R2242	T3576	T3563	parataxis	green,nuclei
R2243	T3577	T3576	punct	),green
R2244	T3578	T3563	prep	in,nuclei
R2245	T3579	T3580	det	the,regions
R2246	T3580	T3578	pobj	regions,in
R2247	T3581	T3582	amod	anterior,epithelium
R2248	T3582	T3580	nmod	epithelium,regions
R2249	T3583	T3582	punct	", ",epithelium
R225	T380	T378	advmod	also,observed
R2250	T3584	T3585	amod	equatorial,bow
R2251	T3585	T3582	conj	bow,epithelium
R2252	T3586	T3585	punct	/,bow
R2253	T3587	T3585	punct	", ",bow
R2254	T3588	T3585	cc	and,bow
R2255	T3589	T3590	amod	posterior,subcapsular
R2256	T3590	T3585	conj	subcapsular,bow
R2257	T3591	T3592	punct	(,2A
R2258	T3592	T3563	parataxis	2A,nuclei
R2259	T3593	T3592	compound	Figure,2A
R226	T381	T378	prep	in,observed
R2260	T3594	T3592	punct	),2A
R2261	T3595	T3563	punct	.,nuclei
R2262	T3597	T3598	compound	Cell,nuclei
R2263	T3598	T3599	nsubjpass	nuclei,disorganized
R2264	T3600	T3598	acl	localized,nuclei
R2265	T3601	T3600	prep	to,localized
R2266	T3602	T3603	det	the,region
R2267	T3603	T3601	pobj	region,to
R2268	T3604	T3605	amod	equatorial,bow
R2269	T3605	T3603	amod	bow,region
R227	T382	T383	det	the,regions
R2270	T3606	T3605	punct	/,bow
R2271	T3607	T3599	auxpass	were,disorganized
R2272	T3608	T3609	mark	as,compared
R2273	T3609	T3599	advcl	compared,disorganized
R2274	T3610	T3609	prep	to,compared
R2275	T3611	T3612	amod	wild,type
R2276	T3612	T3610	pobj	type,to
R2277	T3613	T3599	punct	.,disorganized
R2278	T3615	T3616	expl	There,were
R2279	T3617	T3618	amod	enlarged,spaces
R228	T383	T381	pobj	regions,in
R2280	T3618	T3616	attr	spaces,were
R2281	T3619	T3618	amod	extracellular,spaces
R2282	T3620	T3616	prep	between,were
R2283	T3621	T3620	pobj	cells,between
R2284	T3622	T3616	prep	in,were
R2285	T3623	T3624	det	the,region
R2286	T3624	T3622	pobj	region,in
R2287	T3625	T3626	amod	equatorial,bow
R2288	T3626	T3624	amod	bow,region
R2289	T3627	T3626	punct	/,bow
R229	T384	T385	amod	equatorial,bow
R2290	T3628	T3616	prep	in,were
R2291	T3629	T3630	nummod	5,wk
R2292	T3630	T3631	npadvmod	wk,old
R2293	T3631	T3632	amod	old,lenses
R2294	T3632	T3628	pobj	lenses,in
R2295	T3633	T3634	compound	alphaA,BKO
R2296	T3634	T3632	compound	BKO,lenses
R2297	T3635	T3634	punct	/,BKO
R2298	T3636	T3616	punct	.,were
R2299	T3638	T3639	compound	Fifty,four
R23	T160	T159	pcomp	resolving,to
R230	T385	T383	amod	bow,regions
R2300	T3639	T3641	nummod	four,wk
R2301	T3640	T3639	punct	-,four
R2302	T3641	T3642	npadvmod	wk,old
R2303	T3642	T3643	amod	old,lenses
R2304	T3643	T3647	nsubj	lenses,stain
R2305	T3644	T3645	compound	alphaA,BKO
R2306	T3645	T3643	compound	BKO,lenses
R2307	T3646	T3645	punct	/,BKO
R2308	T3647	T3654	ccomp	stain,stained
R2309	T3648	T3649	punct	(,2B
R231	T386	T385	punct	/,bow
R2310	T3649	T3643	parataxis	2B,lenses
R2311	T3650	T3649	compound	Figure,2B
R2312	T3651	T3649	punct	),2B
R2313	T3652	T3647	aux	did,stain
R2314	T3653	T3647	neg	not,stain
R2315	T3655	T3647	prep	for,stain
R2316	T3656	T3657	amod	nucleic,acid
R2317	T3657	T3655	pobj	acid,for
R2318	T3658	T3647	prep	in,stain
R2319	T3659	T3660	det	the,region
R232	T387	T385	punct	", ",bow
R2320	T3660	T3658	pobj	region,in
R2321	T3661	T3660	amod	posterior,region
R2322	T3662	T3660	amod	subcapsular,region
R2323	T3663	T3654	punct	", ",stained
R2324	T3664	T3654	advmod	however,stained
R2325	T3665	T3654	punct	", ",stained
R2326	T3666	T3667	det	the,region
R2327	T3667	T3654	nsubj	region,stained
R2328	T3668	T3667	amod	entire,region
R2329	T3669	T3667	amod	anterior,region
R233	T388	T385	conj	posterior,bow
R2330	T3670	T3671	amod	subcapsular,lens
R2331	T3671	T3667	compound	lens,region
R2332	T3672	T3654	prep	for,stained
R2333	T3673	T3674	amod	nucleic,acid
R2334	T3674	T3672	pobj	acid,for
R2335	T3675	T3676	punct	(,refer
R2336	T3676	T3654	parataxis	refer,stained
R2337	T3677	T3676	prep	to,refer
R2338	T3678	T3679	compound	Figure,3I
R2339	T3679	T3677	pobj	3I,to
R234	T389	T388	cc	and,posterior
R2340	T3680	T3676	prep	for,refer
R2341	T3681	T3682	amod	high,image
R2342	T3682	T3680	pobj	image,for
R2343	T3683	T3684	compound	magnification,resolution
R2344	T3684	T3682	compound	resolution,image
R2345	T3685	T3684	punct	/,resolution
R2346	T3686	T3682	prep	of,image
R2347	T3687	T3688	det	this,area
R2348	T3688	T3686	pobj	area,of
R2349	T3689	T3676	punct	),refer
R235	T390	T388	conj	anterior,posterior
R2350	T3690	T3654	punct	.,stained
R2351	T3692	T3693	det	A,region
R2352	T3693	T3698	nsubjpass	region,observed
R2353	T3694	T3693	amod	defined,region
R2354	T3695	T3696	amod	equatorial,bow
R2355	T3696	T3693	amod	bow,region
R2356	T3697	T3696	punct	/,bow
R2357	T3699	T3698	auxpass	was,observed
R2358	T3700	T3698	neg	not,observed
R2359	T3701	T3698	prep	in,observed
R236	T391	T383	prep	of,regions
R2360	T3702	T3703	det	these,lenses
R2361	T3703	T3701	pobj	lenses,in
R2362	T3704	T3703	amod	older,lenses
R2363	T3705	T3698	punct	", ",observed
R2364	T3706	T3698	cc	and,observed
R2365	T3707	T3708	compound	fiber,cells
R2366	T3708	T3709	nsubjpass	cells,found
R2367	T3709	T3698	conj	found,observed
R2368	T3710	T3708	acl	extending,cells
R2369	T3711	T3710	prep	to,extending
R237	T392	T391	pobj	lenses,of
R2370	T3712	T3713	det	the,capsule
R2371	T3713	T3711	pobj	capsule,to
R2372	T3714	T3713	amod	posterior,capsule
R2373	T3715	T3713	prep	of,capsule
R2374	T3716	T3717	det	the,lens
R2375	T3717	T3715	pobj	lens,of
R2376	T3718	T3709	auxpass	were,found
R2377	T3719	T3709	neg	not,found
R2378	T3720	T3709	punct	.,found
R2379	T3722	T3723	amod	Disorganized,material
R238	T393	T392	prep	from,lenses
R2380	T3723	T3725	nsubj	material,capped
R2381	T3724	T3723	amod	cellular,material
R2382	T3726	T3727	det	the,region
R2383	T3727	T3725	dobj	region,capped
R2384	T3728	T3727	amod	anterior,region
R2385	T3729	T3727	prep	of,region
R2386	T3730	T3731	det	the,nucleus
R2387	T3731	T3729	pobj	nucleus,of
R2388	T3732	T3733	amod	embryonic,fetal
R2389	T3733	T3731	amod	fetal,nucleus
R239	T394	T395	compound	alphaA,BKO
R2390	T3734	T3733	punct	/,fetal
R2391	T3735	T3725	punct	", ",capped
R2392	T3736	T3725	cc	and,capped
R2393	T3737	T3738	det	a,indentation
R2394	T3738	T3740	nsubj	indentation,was
R2395	T3739	T3738	amod	distinctive,indentation
R2396	T3740	T3725	conj	was,capped
R2397	T3741	T3738	prep	of,indentation
R2398	T3742	T3743	det	the,mass
R2399	T3743	T3741	pobj	mass,of
R24	T161	T160	dobj	some,resolving
R240	T395	T397	compound	BKO,mice
R2400	T3744	T3743	compound	lens,mass
R2401	T3745	T3738	prep	at,indentation
R2402	T3746	T3747	det	the,region
R2403	T3747	T3745	pobj	region,at
R2404	T3748	T3747	amod	equatorial,region
R2405	T3749	T3740	acomp	apparent,was
R2406	T3750	T3740	prep	in,was
R2407	T3751	T3752	amod	mid-axial,sections
R2408	T3752	T3750	pobj	sections,in
R2409	T3753	T3754	advmod	as,as
R241	T396	T395	punct	/,BKO
R2410	T3754	T3752	cc	as,sections
R2411	T3755	T3754	advmod	well,as
R2412	T3756	T3757	amod	whole,lenses
R2413	T3757	T3752	conj	lenses,sections
R2414	T3758	T3740	punct	.,was
R2415	T3760	T3761	prep	In,migrated
R2416	T3762	T3763	compound	alphaA,BKO
R2417	T3763	T3765	compound	BKO,lenses
R2418	T3764	T3763	punct	/,BKO
R2419	T3765	T3760	pobj	lenses,In
R242	T397	T393	pobj	mice,from
R2420	T3766	T3767	det	the,nucleus
R2421	T3767	T3761	nsubj	nucleus,migrated
R2422	T3768	T3769	amod	embryonic,fetal
R2423	T3769	T3767	amod	fetal,nucleus
R2424	T3770	T3769	punct	/,fetal
R2425	T3771	T3767	punct	(,nucleus
R2426	T3772	T3773	amod	primary,fibers
R2427	T3773	T3767	appos	fibers,nucleus
R2428	T3774	T3773	compound	lens,fibers
R2429	T3775	T3761	punct	),migrated
R243	T398	T399	mark	as,compared
R2430	T3776	T3761	aux	had,migrated
R2431	T3777	T3761	advmod	posteriorly,migrated
R2432	T3778	T3761	cc	and,migrated
R2433	T3779	T3761	conj	appeared,migrated
R2434	T3780	T3781	aux	to,be
R2435	T3781	T3779	xcomp	be,appeared
R2436	T3782	T3781	prep	in,be
R2437	T3783	T3782	pobj	contact,in
R2438	T3784	T3783	prep	with,contact
R2439	T3785	T3786	det	the,capsule
R244	T399	T383	advcl	compared,regions
R2440	T3786	T3784	pobj	capsule,with
R2441	T3787	T3786	amod	posterior,capsule
R2442	T3788	T3761	punct	.,migrated
R2443	T3790	T3791	prep	In,was
R2444	T3792	T3793	nummod	5,wk
R2445	T3793	T3794	npadvmod	wk,old
R2446	T3794	T3801	amod	old,lenses
R2447	T3795	T3793	punct	", ",wk
R2448	T3796	T3797	nummod	46,wk
R2449	T3797	T3793	conj	wk,wk
R245	T400	T399	prep	to,compared
R2450	T3798	T3797	cc	or,wk
R2451	T3799	T3800	nummod	72,wk
R2452	T3800	T3797	conj	wk,wk
R2453	T3801	T3790	pobj	lenses,In
R2454	T3802	T3803	amod	wild,type
R2455	T3803	T3801	compound	type,lenses
R2456	T3804	T3805	punct	(,2C
R2457	T3805	T3801	parataxis	2C,lenses
R2458	T3806	T3805	compound	Figure,2C
R2459	T3807	T3805	punct	",",2C
R246	T401	T402	amod	wild,mice
R2460	T3808	T3805	conj	2D,2C
R2461	T3809	T3808	cc	and,2D
R2462	T3810	T3808	conj	2E,2D
R2463	T3811	T3805	punct	),2C
R2464	T3812	T3791	punct	", ",was
R2465	T3813	T3791	advmod	however,was
R2466	T3814	T3791	punct	", ",was
R2467	T3815	T3791	expl	there,was
R2468	T3816	T3817	det	no,staining
R2469	T3817	T3791	attr	staining,was
R247	T402	T400	pobj	mice,to
R2470	T3818	T3817	amod	posterior,staining
R2471	T3819	T3818	cc	or,posterior
R2472	T3820	T3818	conj	anterior,posterior
R2473	T3821	T3817	amod	subcapsular,staining
R2474	T3822	T3823	amod	nucleic,acid
R2475	T3823	T3817	compound	acid,staining
R2476	T3824	T3791	cc	and,was
R2477	T3825	T3826	det	the,nucleus
R2478	T3826	T3830	nsubj	nucleus,was
R2479	T3827	T3828	amod	embryonic,fetal
R248	T403	T378	punct	.,observed
R2480	T3828	T3826	amod	fetal,nucleus
R2481	T3829	T3828	punct	-,fetal
R2482	T3830	T3791	conj	was,was
R2483	T3831	T3832	advmod	centrally,located
R2484	T3832	T3830	acomp	located,was
R2485	T3833	T3832	punct	", ",located
R2486	T3834	T3832	cc	not,located
R2487	T3835	T3832	conj	in,located
R2488	T3836	T3835	pobj	contact,in
R2489	T3837	T3836	prep	with,contact
R249	T407	T408	det	These,results
R2490	T3838	T3839	det	the,capsule
R2491	T3839	T3837	pobj	capsule,with
R2492	T3840	T3839	amod	posterior,capsule
R2493	T3841	T3791	punct	.,was
R2494	T3848	T3849	compound	i,g
R2495	T3861	T3860	amod	f,tion
R2496	T3862	T3860	amod	ica,tion
R2497	T3868	T3867	cc	a,x
R2498	T3869	T3867	conj	m,x
R2499	T3893	T3892	acl	stained,s
R25	T162	T161	prep	of,some
R250	T408	T409	nsubj	results,indicated
R2500	T3894	T3893	prep	with,stained
R2501	T3895	T3894	pobj	DiI,with
R2502	T3896	T3895	cc	and,DiI
R2503	T3897	T3898	compound	SYTOX,green
R2504	T3898	T3895	conj	green,DiI
R2505	T3899	T3900	amod	ultrastructural,differences
R2506	T3900	T3890	dobj	differences,revealed
R2507	T3901	T3890	prep	between,revealed
R2508	T3902	T3903	compound	alphaA,BKO
R2509	T3903	T3905	compound	BKO,lenses
R251	T410	T411	mark	that,are
R2510	T3904	T3903	punct	/,BKO
R2511	T3905	T3901	pobj	lenses,between
R2512	T3906	T3905	cc	and,lenses
R2513	T3907	T3908	amod	wild,type
R2514	T3908	T3909	compound	type,lenses
R2515	T3909	T3905	conj	lenses,lenses
R2516	T3910	T3911	punct	(,Figure
R2517	T3911	T3890	parataxis	Figure,revealed
R2518	T3912	T3911	nummod	3,Figure
R2519	T3913	T3911	punct	),Figure
R252	T411	T409	advcl	are,indicated
R2520	T3914	T3890	punct	.,revealed
R2521	T3916	T3917	amod	Anterior,staining
R2522	T3917	T3919	nsubj	staining,were
R2523	T3918	T3917	amod	epithelial,staining
R2524	T3920	T3917	prep	in,staining
R2525	T3921	T3922	nummod	5,wk
R2526	T3922	T3923	npadvmod	wk,old
R2527	T3923	T3924	amod	old,BKO
R2528	T3924	T3920	pobj	BKO,in
R2529	T3925	T3924	compound	alphaA,BKO
R253	T412	T413	preconj	both,alphaA
R2530	T3926	T3924	punct	/,BKO
R2531	T3927	T3928	punct	(,3A
R2532	T3928	T3924	parataxis	3A,BKO
R2533	T3929	T3928	compound	Figure,3A
R2534	T3930	T3928	punct	),3A
R2535	T3931	T3924	cc	and,BKO
R2536	T3932	T3933	nummod	5,wk
R2537	T3933	T3934	npadvmod	wk,old
R2538	T3934	T3935	amod	old,type
R2539	T3935	T3937	compound	type,lenses
R254	T413	T414	nmod	alphaA,crystallin
R2540	T3936	T3935	amod	wild,type
R2541	T3937	T3924	conj	lenses,BKO
R2542	T3938	T3919	acomp	similar,were
R2543	T3939	T3940	punct	(,3K
R2544	T3940	T3919	parataxis	3K,were
R2545	T3941	T3940	compound	Figure,3K
R2546	T3942	T3940	punct	),3K
R2547	T3943	T3919	prep	with,were
R2548	T3944	T3943	pobj	respect,with
R2549	T3945	T3944	prep	to,respect
R255	T414	T411	nsubj	crystallin,are
R2550	T3946	T3947	amod	nuclear,staining
R2551	T3947	T3945	pobj	staining,to
R2552	T3948	T3947	prep	with,staining
R2553	T3949	T3950	compound	SYTOX,green
R2554	T3950	T3948	pobj	green,with
R2555	T3951	T3919	punct	.,were
R2556	T3953	T3954	advmod	However,observed
R2557	T3955	T3954	punct	", ",observed
R2558	T3956	T3957	det	some,differences
R2559	T3957	T3954	nsubjpass	differences,observed
R256	T415	T413	punct	-,alphaA
R2560	T3958	T3957	prep	in,differences
R2561	T3959	T3960	nummod	54,wk
R2562	T3960	T3961	npadvmod	wk,old
R2563	T3961	T3962	amod	old,lenses
R2564	T3962	T3958	pobj	lenses,in
R2565	T3963	T3964	compound	alphaA,BKO
R2566	T3964	T3962	compound	BKO,lenses
R2567	T3965	T3964	punct	/,BKO
R2568	T3966	T3967	punct	(,3F
R2569	T3967	T3957	parataxis	3F,differences
R257	T416	T413	cc	and,alphaA
R2570	T3968	T3967	compound	Figure,3F
R2571	T3969	T3967	punct	),3F
R2572	T3970	T3954	auxpass	were,observed
R2573	T3971	T3954	prep	in,observed
R2574	T3972	T3973	det	the,epithelium
R2575	T3973	T3971	pobj	epithelium,in
R2576	T3974	T3973	amod	central,epithelium
R2577	T3975	T3973	amod	anterior,epithelium
R2578	T3976	T3954	prep	compared,observed
R2579	T3977	T3976	prep	to,compared
R258	T417	T413	conj	alphaB,alphaA
R2580	T3978	T3979	nummod	5,wk
R2581	T3979	T3980	nmod	wk,lenses
R2582	T3980	T3977	pobj	lenses,to
R2583	T3981	T3982	punct	(,3K
R2584	T3982	T3979	parataxis	3K,wk
R2585	T3983	T3982	compound	Figure,3K
R2586	T3984	T3982	punct	),3K
R2587	T3985	T3979	punct	", ",wk
R2588	T3986	T3987	nummod	46,wk
R2589	T3987	T3979	conj	wk,wk
R259	T418	T414	punct	-,crystallin
R2590	T3988	T3989	punct	(,3P
R2591	T3989	T3987	parataxis	3P,wk
R2592	T3990	T3989	compound	Figure,3P
R2593	T3991	T3989	punct	),3P
R2594	T3992	T3987	punct	", ",wk
R2595	T3993	T3987	cc	and,wk
R2596	T3994	T3995	nummod	72,wk
R2597	T3995	T3987	conj	wk,wk
R2598	T3996	T3997	punct	(,shown
R2599	T3997	T3995	parataxis	shown,wk
R26	T163	T164	det	the,functions
R260	T419	T411	acomp	necessary,are
R2600	T3998	T3997	nsubj	data,shown
R2601	T3999	T3997	neg	not,shown
R2602	T4000	T3997	punct	),shown
R2603	T4001	T4002	amod	wild,type
R2604	T4002	T3980	compound	type,lenses
R2605	T4003	T3954	punct	.,observed
R2606	T4005	T4006	det	These,differences
R2607	T4006	T4007	nsubj	differences,included
R2608	T4008	T4007	dobj	changes,included
R2609	T4009	T4008	prep	in,changes
R261	T420	T419	prep	for,necessary
R2610	T4010	T4011	amod	central,staining
R2611	T4011	T4009	pobj	staining,in
R2612	T4012	T4011	amod	epithelial,staining
R2613	T4013	T4011	amod	nuclear,staining
R2614	T4014	T4011	punct	", ",staining
R2615	T4015	T4016	amod	nuclear,shape
R2616	T4016	T4011	conj	shape,staining
R2617	T4017	T4016	cc	and,shape
R2618	T4018	T4019	amod	epithelial,thickness
R2619	T4019	T4016	conj	thickness,shape
R262	T421	T422	amod	proper,formation
R2620	T4020	T4019	cc	and,thickness
R2621	T4021	T4019	conj	continuity,thickness
R2622	T4022	T4007	punct	.,included
R2623	T4024	T4025	amod	Superficial,staining
R2624	T4025	T4030	nsubj	staining,was
R2625	T4026	T4024	cc	and,Superficial
R2626	T4027	T4024	conj	deep,Superficial
R2627	T4028	T4025	amod	anterior,staining
R2628	T4029	T4025	amod	cortical,staining
R2629	T4031	T4032	advmod	grossly,different
R263	T422	T420	pobj	formation,for
R2630	T4032	T4030	acomp	different,was
R2631	T4033	T4030	prep	between,was
R2632	T4034	T4035	nmod	alphaA,BKO
R2633	T4035	T4037	nmod	BKO,lenses
R2634	T4036	T4035	punct	/,BKO
R2635	T4037	T4033	pobj	lenses,between
R2636	T4038	T4039	punct	(,3A
R2637	T4039	T4035	parataxis	3A,BKO
R2638	T4040	T4039	compound	Figure,3A
R2639	T4041	T4039	punct	",",3A
R264	T423	T424	compound	fiber,cell
R2640	T4042	T4039	conj	3D,3A
R2641	T4043	T4042	punct	",",3D
R2642	T4044	T4042	conj	3F,3D
R2643	T4045	T4044	cc	and,3F
R2644	T4046	T4044	conj	3I,3F
R2645	T4047	T4039	punct	),3A
R2646	T4048	T4035	cc	and,BKO
R2647	T4049	T4050	amod	wild,type
R2648	T4050	T4035	conj	type,BKO
R2649	T4051	T4052	punct	(,3K
R265	T424	T422	compound	cell,formation
R2650	T4052	T4037	parataxis	3K,lenses
R2651	T4053	T4052	compound	Figure,3K
R2652	T4054	T4052	punct	",",3K
R2653	T4055	T4052	conj	3N,3K
R2654	T4056	T4055	punct	",",3N
R2655	T4057	T4055	conj	3P,3N
R2656	T4058	T4057	cc	and,3P
R2657	T4059	T4057	conj	3S,3P
R2658	T4060	T4052	punct	),3K
R2659	T4061	T4030	punct	.,was
R266	T425	T411	punct	", ",are
R2660	T4063	T4064	prep	In,reveal
R2661	T4065	T4066	nummod	5,wk
R2662	T4066	T4069	npadvmod	wk,old
R2663	T4067	T4065	cc	and,5
R2664	T4068	T4065	conj	54,5
R2665	T4069	T4070	amod	old,lenses
R2666	T4070	T4063	pobj	lenses,In
R2667	T4071	T4072	compound	alphaA,BKO
R2668	T4072	T4070	compound	BKO,lenses
R2669	T4073	T4072	punct	/,BKO
R267	T426	T411	cc	and,are
R2670	T4074	T4064	punct	", ",reveal
R2671	T4075	T4076	amod	superficial,regions
R2672	T4076	T4064	nsubj	regions,reveal
R2673	T4077	T4075	cc	and,superficial
R2674	T4078	T4075	conj	deep,superficial
R2675	T4079	T4076	amod	anterior,regions
R2676	T4080	T4076	amod	cortical,regions
R2677	T4081	T4076	punct	", ",regions
R2678	T4082	T4076	acl	stained,regions
R2679	T4083	T4082	prep	for,stained
R268	T427	T428	mark	that,lead
R2680	T4084	T4085	compound	lipid,membranes
R2681	T4085	T4083	pobj	membranes,for
R2682	T4086	T4064	punct	", ",reveal
R2683	T4087	T4064	aux	did,reveal
R2684	T4088	T4064	neg	not,reveal
R2685	T4089	T4090	det	any,patterns
R2686	T4090	T4064	dobj	patterns,reveal
R2687	T4091	T4090	amod	typical,patterns
R2688	T4092	T4091	prep	of,typical
R2689	T4093	T4094	compound	fiber,cells
R269	T428	T411	conj	lead,are
R2690	T4094	T4092	pobj	cells,of
R2691	T4095	T4094	acl	cut,cells
R2692	T4096	T4095	prep	in,cut
R2693	T4097	T4096	pobj	cross-section,in
R2694	T4098	T4097	cc	or,cross-section
R2695	T4099	T4100	amod	longitudinal,section
R2696	T4100	T4097	conj	section,cross-section
R2697	T4101	T4064	punct	.,reveal
R2698	T4103	T4104	prep	In,was
R2699	T4105	T4106	nummod	5,wk
R27	T164	T162	pobj	functions,of
R270	T429	T430	det	the,absence
R2700	T4106	T4107	compound	wk,lenses
R2701	T4107	T4103	pobj	lenses,In
R2702	T4108	T4109	compound	alphaA,BKO
R2703	T4109	T4107	compound	BKO,lenses
R2704	T4110	T4109	punct	/,BKO
R2705	T4111	T4104	expl	there,was
R2706	T4112	T4113	det	a,density
R2707	T4113	T4104	attr	density,was
R2708	T4114	T4113	amod	high,density
R2709	T4115	T4113	prep	of,density
R271	T430	T428	nsubj	absence,lead
R2710	T4116	T4117	amod	stained,membranes
R2711	T4117	T4115	pobj	membranes,of
R2712	T4118	T4113	prep	per,density
R2713	T4119	T4120	compound	unit,area
R2714	T4120	T4118	pobj	area,per
R2715	T4121	T4113	prep	throughout,density
R2716	T4122	T4123	det	the,cortex
R2717	T4123	T4121	pobj	cortex,throughout
R2718	T4124	T4104	punct	", ",was
R2719	T4125	T4104	prep	with,was
R272	T431	T430	prep	of,absence
R2720	T4126	T4127	det	no,staining
R2721	T4127	T4125	pobj	staining,with
R2722	T4128	T4129	amod	nucleic,acid
R2723	T4129	T4127	compound	acid,staining
R2724	T4130	T4127	prep	in,staining
R2725	T4131	T4132	det	these,regions
R2726	T4132	T4130	pobj	regions,in
R2727	T4133	T4104	punct	.,was
R2728	T4135	T4136	prep	In,observed
R2729	T4137	T4138	nummod	54,wk
R273	T432	T433	compound	alpha,crystallin
R2730	T4138	T4139	compound	wk,lenses
R2731	T4139	T4135	pobj	lenses,In
R2732	T4140	T4141	compound	alphaA,BKO
R2733	T4141	T4139	compound	BKO,lenses
R2734	T4142	T4141	punct	/,BKO
R2735	T4143	T4136	punct	", ",observed
R2736	T4144	T4145	amod	large,cells
R2737	T4145	T4136	nsubjpass	cells,observed
R2738	T4146	T4145	punct	", ",cells
R2739	T4147	T4148	advmod	irregularly,shaped
R274	T433	T431	pobj	crystallin,of
R2740	T4148	T4145	amod	shaped,cells
R2741	T4149	T4136	auxpass	were,observed
R2742	T4150	T4136	punct	", ",observed
R2743	T4151	T4136	advcl	interspersed,observed
R2744	T4152	T4151	prep	among,interspersed
R2745	T4153	T4152	pobj	regions,among
R2746	T4154	T4153	prep	of,regions
R2747	T4202	T4200	punct	", ",observed
R2748	T4155	T4156	amod	high,density
R2749	T4203	T4204	amod	nucleic,acid
R275	T434	T433	punct	-,crystallin
R2750	T4156	T4154	pobj	density,of
R2751	T4204	T4205	compound	acid,staining
R2752	T4205	T4200	nsubjpass	staining,observed
R2753	T4206	T4200	auxpass	was,observed
R2754	T4207	T4208	punct	(,3F
R2755	T4208	T4200	parataxis	3F,observed
R2756	T4209	T4208	compound	Figure,3F
R2757	T4157	T4158	compound	membrane,staining
R2758	T4210	T4208	cc	and,3F
R2759	T4211	T4208	conj	3I,3F
R276	T435	T428	aux	can,lead
R2760	T4212	T4208	punct	),3F
R2761	T4213	T4200	prep	within,observed
R2762	T4158	T4156	compound	staining,density
R2763	T4214	T4213	pobj	many,within
R2764	T4215	T4214	prep	of,many
R2765	T4216	T4217	det	these,cells
R2766	T4159	T4156	prep	per,density
R2767	T4217	T4215	pobj	cells,of
R2768	T4218	T4217	acl	exhibiting,cells
R2769	T4219	T4218	amod	large,exhibiting
R277	T436	T428	prep	to,lead
R2770	T4160	T4161	compound	unit,area
R2771	T4220	T4218	amod	cross-sectional,exhibiting
R2772	T4221	T4218	dep	profiles,exhibiting
R2773	T4222	T4200	punct	.,observed
R2774	T4161	T4159	pobj	area,per
R2775	T4224	T4225	prep	In,observed
R2776	T4162	T4136	punct	.,observed
R2777	T4226	T4227	det	the,lenses
R2778	T4227	T4224	pobj	lenses,In
R2779	T4164	T4165	det	These,objects
R278	T437	T438	compound	cataract,formation
R2780	T4228	T4229	amod	wild,type
R2781	T4229	T4227	compound	type,lenses
R2782	T4230	T4225	punct	", ",observed
R2783	T4231	T4232	amod	typical,patterns
R2784	T4165	T4167	nsubj	objects,were
R2785	T4232	T4225	nsubjpass	patterns,observed
R2786	T4233	T4232	amod	cross-sectional,patterns
R2787	T4166	T4165	amod	large,objects
R2788	T4234	T4232	prep	of,patterns
R2789	T4235	T4236	compound	fiber,cells
R279	T438	T436	pobj	formation,to
R2790	T4236	T4234	pobj	cells,of
R2791	T4237	T4232	acl	organized,patterns
R2792	T4238	T4237	prep	in,organized
R2793	T4168	T4167	neg	not,were
R2794	T4239	T4240	amod	radial,columns
R2795	T4240	T4238	pobj	columns,in
R2796	T4241	T4225	auxpass	were,observed
R2797	T4242	T4243	punct	(,3K
R2798	T4169	T4167	attr	vacuoles,were
R2799	T4243	T4225	parataxis	3K,observed
R28	T165	T166	advmod	in,vivo
R280	T439	T409	punct	.,indicated
R2800	T4244	T4243	compound	Figure,3K
R2801	T4170	T4167	punct	", ",were
R2802	T4245	T4243	punct	",",3K
R2803	T4246	T4243	conj	3N,3K
R2804	T4247	T4246	punct	",",3N
R2805	T4248	T4246	conj	3P,3N
R2806	T4171	T4172	mark	because,showed
R2807	T4249	T4248	cc	and,3P
R2808	T4250	T4248	conj	3S,3P
R2809	T4172	T4167	advcl	showed,were
R2810	T4251	T4243	punct	),3K
R2811	T4252	T4225	punct	.,observed
R2812	T4173	T4172	nsubj	examination,showed
R2813	T4254	T4255	amod	Ultrastructural,differences
R2814	T4255	T4256	nsubjpass	differences,observed
R2815	T4174	T4173	prep	of,examination
R2816	T4257	T4255	prep	at,differences
R2817	T4258	T4259	det	the,region
R2818	T4259	T4257	pobj	region,at
R2819	T4175	T4176	det	the,interior
R282	T137	T138	amod	Morphological,characterization
R2820	T4260	T4261	amod	equatorial,bow
R2821	T4261	T4259	amod	bow,region
R2822	T4176	T4174	pobj	interior,of
R2823	T4262	T4261	punct	/,bow
R2824	T4263	T4256	auxpass	were,observed
R2825	T4264	T4256	advmod	also,observed
R2826	T4177	T4176	prep	of,interior
R2827	T4265	T4256	prep	between,observed
R2828	T4266	T4267	compound	alphaA,BKO
R2829	T4267	T4269	compound	BKO,lenses
R2830	T4178	T4179	det	these,structures
R2831	T4268	T4267	punct	/,BKO
R2832	T4269	T4265	pobj	lenses,between
R2833	T4270	T4271	punct	(,3B
R2834	T4271	T4269	parataxis	3B,lenses
R2835	T4179	T4177	pobj	structures,of
R2836	T4272	T4271	compound	Figure,3B
R2837	T4273	T4271	cc	and,3B
R2838	T4274	T4271	conj	3G,3B
R2839	T4180	T4173	prep	by,examination
R2840	T4275	T4271	punct	),3B
R2841	T4276	T4269	cc	and,lenses
R2842	T4181	T4182	amod	transmitted,microscopy
R2843	T4277	T4278	amod	wild,type
R2844	T4278	T4279	compound	type,lenses
R2845	T4279	T4269	conj	lenses,lenses
R2846	T4280	T4281	punct	(,3L
R2847	T4182	T4180	pobj	microscopy,by
R2848	T4281	T4279	parataxis	3L,lenses
R2849	T4282	T4281	compound	Figure,3L
R2850	T4283	T4281	cc	and,3L
R2851	T4183	T4181	cc	and,transmitted
R2852	T4284	T4281	conj	3Q,3L
R2853	T4285	T4281	punct	),3L
R2854	T4286	T4256	punct	.,observed
R2855	T4184	T4181	conj	reflected,transmitted
R2856	T4288	T4289	prep	In,contained
R2857	T4290	T4288	pobj	contrast,In
R2858	T4291	T4290	prep	to,contrast
R2859	T4185	T4182	amod	light,microscopy
R2860	T4292	T4293	amod	wild,type
R2861	T4293	T4294	compound	type,lenses
R2862	T4294	T4291	pobj	lenses,to
R2863	T4295	T4296	punct	(,3L
R2864	T4186	T4187	mark	that,encompassed
R2865	T4296	T4294	parataxis	3L,lenses
R2866	T4297	T4296	compound	Figure,3L
R2867	T4187	T4172	ccomp	encompassed,showed
R2868	T4298	T4296	cc	and,3L
R2869	T4299	T4296	conj	3Q,3L
R2870	T4300	T4296	punct	),3L
R2871	T4188	T4189	det	the,membranes
R2872	T4301	T4289	punct	", ",contained
R2873	T4302	T4303	det	the,lenses
R2874	T4303	T4289	nsubj	lenses,contained
R2875	T4304	T4305	compound	alphaA,BKO
R2876	T4189	T4187	nsubj	membranes,encompassed
R2877	T4305	T4303	compound	BKO,lenses
R2878	T4306	T4305	punct	/,BKO
R2879	T4190	T4191	amod	cellular,material
R2880	T4191	T4187	dobj	material,encompassed
R2881	T4192	T4193	punct	(,shown
R2882	T4307	T4308	punct	(,3B
R2883	T4193	T4172	parataxis	shown,showed
R2884	T4308	T4303	parataxis	3B,lenses
R2885	T4309	T4308	compound	Figure,3B
R2886	T4194	T4193	nsubj	data,shown
R2887	T4310	T4308	cc	and,3B
R2888	T4311	T4308	conj	3G,3B
R2889	T4312	T4308	punct	),3B
R289	T815	T816	compound	Alpha,Crystallin
R2890	T4195	T4193	neg	not,shown
R2891	T4313	T4314	amod	large,areas
R2892	T4314	T4289	dobj	areas,contained
R2893	T4315	T4314	amod	devoid,areas
R2894	T4196	T4172	punct	),showed
R2895	T4316	T4315	prep	of,devoid
R2896	T4317	T4318	amod	cellular,material
R2897	T4197	T4167	punct	.,were
R2898	T4318	T4316	pobj	material,of
R2899	T4319	T4289	punct	", ",contained
R29	T166	T164	amod	vivo,functions
R290	T816	T818	nsubjpass	Crystallin,comprised
R2900	T4320	T4321	poss	their,nuclei
R2901	T4199	T4200	prep	In,observed
R2902	T4321	T4322	nsubjpass	nuclei,limited
R2903	T4322	T4289	conj	limited,contained
R2904	T4323	T4322	auxpass	were,limited
R2905	T4324	T4322	neg	not,limited
R2906	T4201	T4199	pobj	addition,In
R2907	T4325	T4322	prep	to,limited
R2908	T4326	T4327	det	a,region
R2909	T4327	T4325	pobj	region,to
R291	T817	T816	punct	-,Crystallin
R2910	T4328	T4329	advmod	well,defined
R2911	T4329	T4327	amod	defined,region
R2912	T4330	T4331	punct	equatorial,bow
R2913	T4331	T4327	amod	bow,region
R2914	T4332	T4331	punct	/,bow
R2915	T4412	T4413	det	a,variation
R2916	T4413	T4400	nsubjpass	variation,observed
R2917	T4333	T4322	punct	", ",limited
R2918	T4414	T4413	amod	greater,variation
R2919	T4334	T4322	cc	and,limited
R292	T819	T818	auxpass	is,comprised
R2920	T4415	T4413	prep	in,variation
R2921	T4335	T4336	amod	ordered,columns
R2922	T4336	T4338	nsubjpass	columns,observed
R2923	T4416	T4417	advmod	cross,sectional
R2924	T4337	T4336	amod	radial,columns
R2925	T4338	T4322	conj	observed,limited
R2926	T4417	T4418	amod	sectional,diameter
R2927	T4339	T4336	prep	of,columns
R2928	T4340	T4341	amod	elongating,cells
R2929	T4341	T4339	pobj	cells,of
R293	T820	T818	prep	of,comprised
R2930	T4342	T4341	compound	fiber,cells
R2931	T4418	T4415	pobj	diameter,in
R2932	T4343	T4336	acl	extending,columns
R2933	T4344	T4343	prep	from,extending
R2934	T4419	T4400	auxpass	was,observed
R2935	T4345	T4346	det	the,capsule
R2936	T4346	T4344	pobj	capsule,from
R2937	T4347	T4346	amod	posterior,capsule
R2938	T4348	T4343	prep	to,extending
R2939	T4420	T4400	punct	", ",observed
R294	T821	T822	nummod	two,polypeptides
R2940	T4349	T4350	det	the,epithelium
R2941	T4350	T4348	pobj	epithelium,to
R2942	T4421	T4400	prep	with,observed
R2943	T4351	T4350	amod	anterior,epithelium
R2944	T4352	T4338	auxpass	were,observed
R2945	T4353	T4338	neg	not,observed
R2946	T4354	T4289	punct	.,contained
R2947	T4422	T4423	compound	cell,diameters
R2948	T4423	T4424	nsubj	diameters,ranging
R2949	T4356	T4357	prep	In,observed
R295	T822	T820	pobj	polypeptides,of
R2950	T4358	T4356	pobj	addition,In
R2951	T4359	T4358	prep	to,addition
R2952	T4424	T4421	pcomp	ranging,with
R2953	T4360	T4361	det	the,region
R2954	T4361	T4359	pobj	region,to
R2955	T4362	T4361	amod	equatorial,region
R2956	T4363	T4357	punct	", ",observed
R2957	T4425	T4424	prep	from,ranging
R2958	T4364	T4365	amod	ultrastructural,differences
R2959	T4365	T4357	nsubjpass	differences,observed
R296	T823	T822	punct	", ",polypeptides
R2960	T4366	T4365	prep	between,differences
R2961	T4426	T4427	amod	less,2
R2962	T4367	T4368	compound	alphaA,BKO
R2963	T4368	T4370	compound	BKO,lenses
R2964	T4369	T4368	punct	/,BKO
R2965	T4370	T4366	pobj	lenses,between
R2966	T4371	T4372	punct	(,3C
R2967	T4372	T4370	parataxis	3C,lenses
R2968	T4427	T4429	nummod	2,microns
R2969	T4373	T4372	compound	Figure,3C
R297	T824	T825	compound	alphaA,crystallin
R2970	T4374	T4372	cc	and,3C
R2971	T4375	T4372	conj	3H,3C
R2972	T4376	T4372	punct	),3C
R2973	T4428	T4427	quantmod	than,2
R2974	T4377	T4370	cc	and,lenses
R2975	T4378	T4379	amod	wild,type
R2976	T4379	T4380	compound	type,lenses
R2977	T4429	T4425	pobj	microns,from
R2978	T4380	T4370	conj	lenses,lenses
R2979	T4381	T4382	punct	(,3M
R298	T825	T822	appos	crystallin,polypeptides
R2980	T4430	T4431	advmod	up,to
R2981	T4382	T4380	parataxis	3M,lenses
R2982	T4383	T4382	compound	Figure,3M
R2983	T4384	T4382	cc	and,3M
R2984	T4385	T4382	conj	3R,3M
R2985	T4431	T4425	prep	to,from
R2986	T4386	T4382	punct	),3M
R2987	T4387	T4357	auxpass	were,observed
R2988	T4388	T4357	prep	in,observed
R2989	T4432	T4433	advmod	approximately,25
R299	T826	T825	punct	-,crystallin
R2990	T4389	T4390	compound	fiber,cells
R2991	T4390	T4388	pobj	cells,in
R2992	T4391	T4390	prep	of,cells
R2993	T4433	T4434	nummod	25,microns
R2994	T4392	T4393	det	the,nucleus
R2995	T4393	T4391	pobj	nucleus,of
R2996	T4394	T4393	amod	embryonic,nucleus
R2997	T4434	T4431	pobj	microns,to
R2998	T4395	T4394	cc	and,embryonic
R2999	T4396	T4394	conj	fetal,embryonic
R30	T167	T164	prep	of,functions
R300	T827	T825	punct	(,crystallin
R3000	T4435	T4424	punct	", ",ranging
R3001	T4397	T4357	punct	.,observed
R3002	T4399	T4400	prep	In,observed
R3003	T4436	T4424	prep	compared,ranging
R3004	T4401	T4402	nummod	5,wk
R3005	T4402	T4403	compound	wk,lenses
R3006	T4437	T4436	prep	to,compared
R3007	T4403	T4399	pobj	lenses,In
R3008	T4404	T4405	compound	alphaA,BKO
R3009	T4438	T4439	det	the,lenses
R301	T828	T825	appos	alphaA,crystallin
R3010	T4405	T4403	compound	BKO,lenses
R3011	T4406	T4405	punct	/,BKO
R3012	T4407	T4408	punct	(,3C
R3013	T4408	T4403	parataxis	3C,lenses
R3014	T4409	T4408	compound	Figure,3C
R3015	T4410	T4408	punct	),3C
R3016	T4411	T4400	punct	", ",observed
R3017	T4439	T4437	pobj	lenses,to
R3018	T4440	T4441	nummod	5,wk
R3019	T4441	T4439	nmod	wk,lenses
R302	T829	T825	punct	),crystallin
R3020	T4442	T4441	cc	and,wk
R3021	T4518	T4519	compound	alphaA,BKO
R3022	T4519	T4517	compound	BKO,lenses
R3023	T4443	T4444	nummod	46,wk
R3024	T4520	T4519	punct	/,BKO
R3025	T4521	T4522	punct	(,3E
R3026	T4522	T4517	parataxis	3E,lenses
R3027	T4444	T4441	conj	wk,wk
R3028	T4523	T4522	compound	Figure,3E
R3029	T4524	T4522	punct	),3E
R303	T830	T825	cc	and,crystallin
R3030	T4445	T4446	amod	wild,type
R3031	T4525	T4526	amod	nucleic,acid
R3032	T4526	T4527	compound	acid,staining
R3033	T4527	T4508	dobj	staining,exhibited
R3034	T4528	T4508	punct	", ",exhibited
R3035	T4446	T4439	compound	type,lenses
R3036	T4529	T4508	dep	were,exhibited
R3037	T4530	T4529	acomp	small,were
R3038	T4531	T4530	cc	and,small
R3039	T4447	T4439	punct	", ",lenses
R304	T831	T832	compound	alphaB,crystallin
R3040	T4532	T4530	conj	non-elongated,small
R3041	T4533	T4529	punct	", ",were
R3042	T4534	T4529	prep	with,were
R3043	T4448	T4449	poss	whose,diameters
R3044	T4535	T4536	amod	numerous,projections
R3045	T4536	T4534	pobj	projections,with
R3046	T4537	T4536	compound	membrane,projections
R3047	T4449	T4450	dep	diameters,ranged
R3048	T4538	T4508	punct	.,exhibited
R3049	T4539	T4540	punct	", ",observed
R305	T832	T825	conj	crystallin,crystallin
R3050	T4450	T4439	relcl	ranged,lenses
R3051	T4542	T4540	nsubjpass	These,observed
R3052	T4451	T4450	prep	from,ranged
R3053	T4543	T4540	auxpass	were,observed
R3054	T4544	T4540	punct	", ",observed
R3055	T4545	T4540	advmod	however,observed
R3056	T4452	T4453	amod	less,2
R3057	T4546	T4540	punct	", ",observed
R3058	T4547	T4540	neg	not,observed
R3059	T4548	T4540	prep	in,observed
R306	T833	T832	punct	-,crystallin
R3060	T4453	T4455	nummod	2,microns
R3061	T4549	T4550	nummod	54,wk
R3062	T4550	T4551	compound	wk,lenses
R3063	T4551	T4548	pobj	lenses,in
R3064	T4454	T4453	quantmod	than,2
R3065	T4552	T4553	compound	alphaA,BKO
R3066	T4553	T4551	compound	BKO,lenses
R3067	T4554	T4553	punct	/,BKO
R3068	T4555	T4540	punct	.,observed
R3069	T4557	T4558	prep	In,observed
R307	T834	T832	punct	(,crystallin
R3070	T4455	T4451	pobj	microns,from
R3071	T4559	T4560	nummod	5,wk
R3072	T4560	T4561	compound	wk,lenses
R3073	T4456	T4457	advmod	up,to
R3074	T4561	T4557	pobj	lenses,In
R3075	T4562	T4563	compound	alphaA,BKO
R3076	T4563	T4561	compound	BKO,lenses
R3077	T4457	T4451	prep	to,from
R3078	T4564	T4563	punct	/,BKO
R3079	T4565	T4558	punct	", ",observed
R308	T835	T832	appos	alphaB,crystallin
R3080	T4566	T4558	advmod	deep,observed
R3081	T4458	T4459	advmod	approximately,10
R3082	T4567	T4566	prep	to,deep
R3083	T4568	T4569	det	the,capsule
R3084	T4569	T4567	pobj	capsule,to
R3085	T4459	T4460	nummod	10,microns
R3086	T4570	T4569	amod	posterior,capsule
R3087	T4571	T4558	punct	", ",observed
R3088	T4572	T4573	amod	larger,cells
R3089	T4573	T4558	nsubjpass	cells,observed
R309	T836	T822	punct	),polypeptides
R3090	T4460	T4457	pobj	microns,to
R3091	T4574	T4573	compound	diameter,cells
R3092	T4575	T4573	punct	", ",cells
R3093	T4461	T4462	punct	(,3M
R3094	T4576	T4573	amod	similar,cells
R3095	T4577	T4576	prep	to,similar
R3096	T4578	T4577	pobj	those,to
R3097	T4462	T4450	parataxis	3M,ranged
R3098	T4579	T4578	acl	observed,those
R3099	T4580	T4579	prep	in,observed
R31	T168	T169	compound	alpha,crystallin
R310	T837	T822	punct	", ",polypeptides
R3100	T4581	T4582	det	the,nucleus
R3101	T4463	T4462	compound	Figure,3M
R3102	T4582	T4580	pobj	nucleus,in
R3103	T4583	T4584	amod	embryonic,fetal
R3104	T4584	T4582	amod	fetal,nucleus
R3105	T4464	T4462	cc	and,3M
R3106	T4585	T4584	punct	/,fetal
R3107	T4586	T4558	auxpass	were,observed
R3108	T4465	T4462	conj	3R,3M
R3109	T4587	T4588	punct	(,shown
R311	T838	T839	dep	which,share
R3110	T4466	T4462	punct	),3M
R3111	T4588	T4558	parataxis	shown,observed
R3112	T4589	T4588	nsubj	data,shown
R3113	T4590	T4588	neg	not,shown
R3114	T4467	T4400	punct	.,observed
R3115	T4591	T4588	punct	),shown
R3116	T4592	T4558	punct	.,observed
R3117	T4469	T4470	prep	At,were
R3118	T4594	T4595	compound	Fiber,cells
R3119	T4595	T4596	nsubjpass	cells,seen
R312	T839	T822	relcl	share,polypeptides
R3120	T4597	T4595	prep	of,cells
R3121	T4598	T4599	amod	similar,dimension
R3122	T4599	T4597	pobj	dimension,of
R3123	T4471	T4472	nummod	54,wk
R3124	T4600	T4599	cc	and,dimension
R3125	T4601	T4599	conj	appearance,dimension
R3126	T4602	T4599	prep	to,dimension
R3127	T4603	T4604	amod	embryonic,fetal
R3128	T4604	T4606	amod	fetal,fibers
R3129	T4605	T4604	punct	/,fetal
R313	T840	T841	nummod	55,%
R3130	T4472	T4469	pobj	wk,At
R3131	T4606	T4602	pobj	fibers,to
R3132	T4607	T4606	amod	nuclear,fibers
R3133	T4608	T4596	auxpass	were,seen
R3134	T4473	T4470	punct	", ",were
R3135	T4609	T4596	prep	at,seen
R3136	T4610	T4611	det	the,capsule
R3137	T4611	T4609	pobj	capsule,at
R3138	T4474	T4475	det	these,cells
R3139	T4612	T4611	amod	posterior,capsule
R314	T841	T842	compound	%,acid
R3140	T4613	T4596	prep	in,seen
R3141	T4614	T4615	nummod	54,wk
R3142	T4475	T4470	nsubj	cells,were
R3143	T4615	T4616	compound	wk,lenses
R3144	T4616	T4613	pobj	lenses,in
R3145	T4617	T4618	compound	alphaA,BKO
R3146	T4476	T4475	prep	in,cells
R3147	T4618	T4616	compound	BKO,lenses
R3148	T4619	T4618	punct	/,BKO
R3149	T4477	T4478	det	the,lenses
R315	T842	T844	compound	acid,sequence
R3150	T4620	T4621	punct	(,3J
R3151	T4621	T4596	parataxis	3J,seen
R3152	T4622	T4621	compound	Figure,3J
R3153	T4478	T4476	pobj	lenses,in
R3154	T4479	T4480	compound	alphaA,BKO
R3155	T4480	T4478	compound	BKO,lenses
R3156	T4623	T4621	punct	),3J
R3157	T4481	T4480	punct	/,BKO
R3158	T4624	T4596	punct	.,seen
R3159	T4482	T4470	acomp	larger,were
R316	T843	T842	compound	amino,acid
R3160	T4626	T4627	prep	In,found
R3161	T4628	T4629	quantmod	both,5
R3162	T4483	T4470	prep	in,were
R3163	T4629	T4630	nummod	5,wk
R3164	T4484	T4485	amod	cross,sectional
R3165	T4630	T4633	compound	wk,lenses
R3166	T4631	T4629	cc	and,5
R3167	T4632	T4629	conj	54,5
R3168	T4633	T4626	pobj	lenses,In
R3169	T4485	T4486	amod	sectional,diameter
R317	T844	T845	compound	sequence,homology
R3170	T4634	T4635	compound	alphaA,BKO
R3171	T4635	T4633	compound	BKO,lenses
R3172	T4636	T4635	punct	/,BKO
R3173	T4637	T4627	punct	", ",found
R3174	T4638	T4639	det	no,sutures
R3175	T4639	T4627	nsubjpass	sutures,found
R3176	T4486	T4483	pobj	diameter,in
R3177	T4640	T4639	amod	posterior,sutures
R3178	T4641	T4627	aux	could,found
R3179	T4642	T4627	auxpass	be,found
R318	T845	T839	dobj	homology,share
R3180	T4487	T4470	prep	than,were
R3181	T4643	T4627	prep	in,found
R3182	T4644	T4645	det	any,sections
R3183	T4645	T4643	pobj	sections,in
R3184	T4488	T4489	amod	wild,type
R3185	T4646	T4645	amod	axial,sections
R3186	T4647	T4646	cc	or,axial
R3187	T4489	T4487	pobj	type,than
R3188	T4648	T4646	conj	equatorial,axial
R3189	T4649	T4645	compound	vibratome,sections
R319	T846	T847	punct	[,1
R3190	T4650	T4645	acl	examined,sections
R3191	T4490	T4470	punct	", ",were
R3192	T4651	T4627	punct	.,found
R3193	T4653	T4654	amod	Posterior,sutures
R3194	T4491	T4470	cc	and,were
R3195	T4654	T4655	nsubjpass	sutures,found
R3196	T4492	T4470	conj	exhibited,were
R3197	T4656	T4655	auxpass	were,found
R3198	T4657	T4655	advmod	readily,found
R3199	T4658	T4655	prep	in,found
R32	T169	T167	pobj	crystallin,of
R320	T847	T818	parataxis	1,comprised
R3200	T4659	T4660	compound	vibratome,sections
R3201	T4493	T4494	det	a,variation
R3202	T4660	T4658	pobj	sections,in
R3203	T4661	T4660	prep	of,sections
R3204	T4494	T4492	dobj	variation,exhibited
R3205	T4662	T4663	amod	wild,type
R3206	T4663	T4664	compound	type,lenses
R3207	T4664	T4661	pobj	lenses,of
R3208	T4495	T4496	advmod	much,greater
R3209	T4665	T4666	punct	(,arrow
R321	T848	T847	punct	],1
R3210	T4666	T4655	parataxis	arrow,found
R3211	T4667	T4666	dep	Figure,arrow
R3212	T4496	T4494	amod	greater,variation
R3213	T4668	T4667	nummod	3O,Figure
R3214	T4669	T4666	punct	", ",arrow
R3215	T4670	T4666	punct	),arrow
R3216	T4497	T4492	prep	in,exhibited
R3217	T4671	T4655	punct	.,found
R3218	T4673	T4674	compound	Fiber,cells
R3219	T4498	T4499	det	the,size
R322	T849	T818	punct	.,comprised
R3220	T4674	T4675	nsubjpass	cells,observed
R3221	T4676	T4674	acl	attached,cells
R3222	T4677	T4676	prep	to,attached
R3223	T4499	T4497	pobj	size,in
R3224	T4678	T4679	det	the,capsule
R3225	T4500	T4501	advmod	cross,sectional
R3226	T4679	T4677	pobj	capsule,to
R3227	T4680	T4679	amod	posterior,capsule
R3228	T4501	T4499	amod	sectional,size
R3229	T4681	T4676	cc	and,attached
R323	T851	T852	nsubj	They,are
R3230	T4682	T4676	conj	extending,attached
R3231	T4683	T4682	advmod	anteriorly,extending
R3232	T4502	T4499	cc	and,size
R3233	T4684	T4675	auxpass	were,observed
R3234	T4685	T4675	prep	in,observed
R3235	T4686	T4687	nummod	5,wk
R3236	T4503	T4504	compound	cell,shape
R3237	T4687	T4688	nmod	wk,lenses
R3238	T4688	T4685	pobj	lenses,in
R3239	T4504	T4499	conj	shape,size
R324	T853	T854	det	the,proteins
R3240	T4689	T4690	punct	(,3O
R3241	T4690	T4687	parataxis	3O,wk
R3242	T4505	T4470	punct	.,were
R3243	T4691	T4690	compound	Figure,3O
R3244	T4692	T4690	punct	),3O
R3245	T4693	T4687	punct	", ",wk
R3246	T4507	T4508	nsubj	Cells,exhibited
R3247	T4694	T4695	nummod	46,wk
R3248	T4695	T4687	conj	wk,wk
R3249	T4696	T4697	punct	(,3T
R325	T854	T852	attr	proteins,are
R3250	T4697	T4695	parataxis	3T,wk
R3251	T4698	T4697	compound	Figure,3T
R3252	T4509	T4507	amod	adjacent,Cells
R3253	T4699	T4697	punct	),3T
R3254	T4700	T4695	cc	and,wk
R3255	T4701	T4702	nummod	72,wk
R3256	T4702	T4695	conj	wk,wk
R3257	T4703	T4704	punct	(,shown
R3258	T4510	T4509	prep	to,adjacent
R3259	T4704	T4702	parataxis	shown,wk
R326	T855	T856	advmod	most,abundant
R3260	T4705	T4704	nsubj	data,shown
R3261	T4706	T4704	neg	not,shown
R3262	T4511	T4512	det	the,capsule
R3263	T4707	T4704	punct	),shown
R3264	T4708	T4709	amod	wild,type
R3265	T4709	T4688	compound	type,lenses
R3266	T4512	T4510	pobj	capsule,to
R3267	T4710	T4675	punct	.,observed
R3268	T4513	T4512	amod	posterior,capsule
R3269	T4712	T4713	nsubj	SEM,confirmed
R327	T856	T854	amod	abundant,proteins
R3270	T4514	T4512	prep	of,capsule
R3271	T4714	T4713	dobj	many,confirmed
R3272	T4715	T4714	prep	of,many
R3273	T4716	T4717	det	the,observations
R3274	T4717	T4715	pobj	observations,of
R3275	T4718	T4717	acl	made,observations
R3276	T4719	T4718	agent	by,made
R3277	T4515	T4516	nummod	5,wk
R3278	T4720	T4721	amod	confocal,microscopy
R3279	T4516	T4517	compound	wk,lenses
R328	T857	T854	prep	in,proteins
R3280	T4721	T4719	pobj	microscopy,by
R3281	T4517	T4514	pobj	lenses,of
R3282	T4722	T4713	punct	.,confirmed
R3283	T4728	T4729	amod	equatorial,bow
R3284	T4724	T4725	prep	In,observed
R3285	T4726	T4727	det	the,region
R3286	T4729	T4727	amod	bow,region
R3287	T4727	T4724	pobj	region,In
R3288	T4730	T4729	punct	/,bow
R3289	T4731	T4725	punct	", ",observed
R329	T858	T859	compound	lens,fiber
R3290	T4732	T4733	det	a,array
R3291	T4833	T4806	punct	", ",observed
R3292	T4733	T4725	nsubjpass	array,observed
R3293	T4834	T4806	cc	but,observed
R3294	T4835	T4836	det	these,columns
R3295	T4836	T4838	nsubj	columns,were
R3296	T4837	T4836	amod	radial,columns
R3297	T4734	T4733	amod	disorganized,array
R3298	T4838	T4806	conj	were,observed
R3299	T4839	T4840	neg	no,longer
R33	T170	T169	punct	-,crystallin
R330	T859	T860	compound	fiber,cells
R3300	T4840	T4838	advmod	longer,were
R3301	T4735	T4733	prep	of,array
R3302	T4841	T4838	acomp	present,were
R3303	T4842	T4838	prep	at,were
R3304	T4736	T4737	advmod	relatively,small
R3305	T4843	T4844	nummod	54,weeks
R3306	T4844	T4842	pobj	weeks,at
R3307	T4845	T4844	prep	of,weeks
R3308	T4737	T4738	amod	small,cells
R3309	T4738	T4735	pobj	cells,of
R331	T860	T857	pobj	cells,in
R3310	T4846	T4845	pobj	age,of
R3311	T4847	T4848	punct	(,4E
R3312	T4739	T4740	amod	irregular,shaped
R3313	T4848	T4838	parataxis	4E,were
R3314	T4849	T4848	compound	Figure,4E
R3315	T4850	T4848	punct	),4E
R3316	T4740	T4738	amod	shaped,cells
R3317	T4851	T4838	punct	.,were
R3318	T4741	T4725	auxpass	were,observed
R3319	T4853	T4854	prep	In,were
R332	T861	T862	punct	[,3
R3320	T4855	T4856	det	these,lenses
R3321	T4742	T4725	prep	in,observed
R3322	T4856	T4853	pobj	lenses,In
R3323	T4857	T4856	amod	older,lenses
R3324	T4743	T4744	nummod	5,wk
R3325	T4858	T4854	punct	", ",were
R3326	T4859	T4860	advmod	irregularly,shaped
R3327	T4860	T4861	amod	shaped,cells
R3328	T4861	T4854	nsubj	cells,were
R3329	T4862	T4861	punct	", ",cells
R333	T862	T852	parataxis	3,are
R3330	T4744	T4745	nmod	wk,lenses
R3331	T4863	T4861	amod	convoluted,cells
R3332	T4864	T4861	punct	", ",cells
R3333	T4865	T4861	prep	with,cells
R3334	T4745	T4742	pobj	lenses,in
R3335	T4866	T4867	det	no,pattern
R3336	T4867	T4865	pobj	pattern,with
R3337	T4868	T4867	amod	recognizable,pattern
R3338	T4869	T4867	prep	of,pattern
R3339	T4746	T4747	punct	(,4A
R334	T863	T862	nummod	2,3
R3340	T4870	T4869	pobj	organization,of
R3341	T4871	T4854	punct	", ",were
R3342	T4872	T4854	acomp	present,were
R3343	T4747	T4744	parataxis	4A,wk
R3344	T4873	T4854	punct	.,were
R3345	T4875	T4876	prep	In,were
R3346	T4748	T4747	compound	Figure,4A
R3347	T4877	T4875	pobj	contrast,In
R3348	T4878	T4876	punct	", ",were
R3349	T4749	T4747	punct	),4A
R335	T864	T862	punct	",",3
R3350	T4879	T4880	amod	radial,columns
R3351	T4880	T4876	nsubj	columns,were
R3352	T4881	T4880	prep	of,columns
R3353	T4750	T4744	cc	and,wk
R3354	T4882	T4883	amod	elongated,cells
R3355	T4883	T4881	pobj	cells,of
R3356	T4884	T4883	compound	fiber,cells
R3357	T4751	T4752	nummod	54,wk
R3358	T4885	T4883	prep	of,cells
R3359	T4886	T4887	amod	uniform,size
R336	T865	T862	punct	],3
R3360	T4887	T4885	pobj	size,of
R3361	T4752	T4744	conj	wk,wk
R3362	T4888	T4887	cc	and,size
R3363	T4889	T4887	conj	shape,size
R3364	T4890	T4880	punct	", ",columns
R3365	T4891	T4880	prep	with,columns
R3366	T4753	T4754	punct	(,4D
R3367	T4892	T4893	nmod	ball,interdigitations
R3368	T4893	T4891	pobj	interdigitations,with
R3369	T4754	T4752	parataxis	4D,wk
R337	T866	T852	cc	and,are
R3370	T4894	T4892	cc	and,ball
R3371	T4895	T4892	conj	socket,ball
R3372	T4896	T4876	punct	", ",were
R3373	T4897	T4876	acomp	present,were
R3374	T4755	T4754	compound	Figure,4D
R3375	T4898	T4876	prep	in,were
R3376	T4899	T4900	det	the,region
R3377	T4756	T4754	punct	),4D
R3378	T4900	T4898	pobj	region,in
R3379	T4901	T4900	amod	anterior,region
R338	T867	T852	conj	exist,are
R3380	T4902	T4900	amod	cortical,region
R3381	T4903	T4900	prep	of,region
R3382	T4904	T4905	nummod	5,wk
R3383	T4905	T4906	nmod	wk,lenses
R3384	T4757	T4758	compound	alphaA,BKO
R3385	T4906	T4903	pobj	lenses,of
R3386	T4907	T4908	punct	(,4H
R3387	T4908	T4905	parataxis	4H,wk
R3388	T4909	T4908	compound	Figure,4H
R3389	T4758	T4745	compound	BKO,lenses
R339	T868	T867	prep	as,exist
R3390	T4910	T4908	punct	),4H
R3391	T4911	T4905	punct	", ",wk
R3392	T4912	T4913	nummod	46,wk
R3393	T4759	T4758	punct	/,BKO
R3394	T4913	T4905	conj	wk,wk
R3395	T4914	T4915	punct	(,4K
R3396	T4915	T4913	parataxis	4K,wk
R3397	T4760	T4725	punct	", ",observed
R3398	T4916	T4915	compound	Figure,4K
R3399	T4917	T4915	punct	),4K
R34	T171	T172	aux	to,generate
R340	T869	T868	pobj	heteroaggregates,as
R3400	T4918	T4913	cc	and,wk
R3401	T4761	T4762	mark	while,observed
R3402	T4919	T4920	nummod	72,week
R3403	T4920	T4913	conj	week,wk
R3404	T4921	T4922	punct	(,shown
R3405	T4762	T4725	advcl	observed,observed
R3406	T4922	T4920	parataxis	shown,week
R3407	T4923	T4922	nsubj	data,shown
R3408	T4924	T4922	neg	not,shown
R3409	T4763	T4764	amod	elongated,cells
R341	T870	T869	prep	of,heteroaggregates
R3410	T4925	T4922	punct	),shown
R3411	T4926	T4927	amod	wild,type
R3412	T4927	T4906	compound	type,lenses
R3413	T4764	T4762	nsubjpass	cells,observed
R3414	T4928	T4876	punct	.,were
R3415	T4930	T4931	det	The,regions
R3416	T4765	T4764	compound	fiber,cells
R3417	T4766	T4764	acl	organized,cells
R3418	T4931	T4934	nsubjpass	regions,filled
R3419	T4932	T4931	amod	posterior,regions
R342	T871	T872	advmod	approximately,800
R3420	T4933	T4931	amod	subcapsular,regions
R3421	T4767	T4766	prep	into,organized
R3422	T4935	T4931	prep	of,regions
R3423	T4936	T4937	nummod	5,wk
R3424	T4768	T4769	amod	radial,columns
R3425	T4937	T4938	nmod	wk,lenses
R3426	T4938	T4935	pobj	lenses,of
R3427	T4769	T4767	pobj	columns,into
R3428	T4770	T4764	punct	", ",cells
R3429	T4771	T4764	prep	with,cells
R343	T872	T873	nummod	800,kDa
R3430	T4939	T4940	punct	(,4C
R3431	T4940	T4937	parataxis	4C,wk
R3432	T4941	T4940	compound	Figure,4C
R3433	T4942	T4940	punct	),4C
R3434	T4943	T4937	cc	and,wk
R3435	T4772	T4773	nmod	ball,interdigitations
R3436	T4944	T4945	nummod	54,wk
R3437	T4945	T4937	conj	wk,wk
R3438	T4946	T4947	punct	(,4F
R3439	T4773	T4771	pobj	interdigitations,with
R344	T873	T870	pobj	kDa,of
R3440	T4947	T4945	parataxis	4F,wk
R3441	T4948	T4947	compound	Figure,4F
R3442	T4949	T4947	punct	),4F
R3443	T4774	T4772	cc	and,ball
R3444	T4950	T4951	compound	alphaA,BKO
R3445	T4951	T4938	compound	BKO,lenses
R3446	T4952	T4951	punct	/,BKO
R3447	T4775	T4772	conj	socket,ball
R3448	T4953	T4934	auxpass	were,filled
R3449	T4954	T4934	prep	with,filled
R345	T874	T875	dep	that,undergo
R3450	T4955	T4956	advmod	irregularly,shaped
R3451	T4776	T4762	punct	", ",observed
R3452	T4956	T4957	amod	shaped,cells
R3453	T4957	T4954	pobj	cells,with
R3454	T4958	T4957	prep	with,cells
R3455	T4777	T4762	auxpass	were,observed
R3456	T4959	T4960	amod	numerous,projections
R3457	T4960	T4958	pobj	projections,with
R3458	T4961	T4960	compound	cell,projections
R3459	T4778	T4762	prep	in,observed
R346	T875	T869	relcl	undergo,heteroaggregates
R3460	T4962	T4960	compound	surface,projections
R3461	T4963	T4934	punct	.,filled
R3462	T4779	T4780	nummod	5,wk
R3463	T4965	T4966	prep	In,were
R3464	T4967	T4968	det	the,nucleus
R3465	T4780	T4781	nmod	wk,lenses
R3466	T4968	T4965	pobj	nucleus,In
R3467	T4969	T4970	amod	fetal,embryonic
R3468	T4970	T4968	amod	embryonic,nucleus
R3469	T4781	T4778	pobj	lenses,in
R347	T876	T875	aux	can,undergo
R3470	T4971	T4970	punct	/,embryonic
R3471	T4972	T4966	punct	", ",were
R3472	T4973	T4974	amod	elongated,cells
R3473	T4782	T4783	punct	(,4G
R3474	T4974	T4966	nsubj	cells,were
R3475	T4975	T4974	compound	fiber,cells
R3476	T4976	T4974	prep	with,cells
R3477	T4783	T4780	parataxis	4G,wk
R3478	T4977	T4978	amod	numerous,projections
R3479	T4978	T4976	pobj	projections,with
R348	T877	T878	amod	inter-aggregate,exchange
R3480	T4979	T4978	amod	long,projections
R3481	T4784	T4783	compound	Figure,4G
R3482	T4980	T4978	punct	", ",projections
R3483	T4981	T4982	amod	finger,like
R3484	T4982	T4978	amod	like,projections
R3485	T4983	T4982	punct	-,like
R3486	T4984	T4978	cc	and,projections
R3487	T4985	T4986	amod	furrowed,membranes
R3488	T4785	T4783	punct	),4G
R3489	T4986	T4978	conj	membranes,projections
R349	T878	T875	dobj	exchange,undergo
R3490	T4987	T4966	acomp	evident,were
R3491	T4786	T4780	punct	", ",wk
R3492	T4988	T4989	punct	(,shown
R3493	T4989	T4966	parataxis	shown,were
R3494	T4990	T4989	neg	not,shown
R3495	T4991	T4989	punct	),shown
R3496	T4787	T4788	nummod	46,wk
R3497	T4992	T4966	punct	.,were
R3498	T4994	T4995	det	No,cells
R3499	T4788	T4780	conj	wk,wk
R35	T172	T158	xcomp	generate,is
R350	T879	T878	compound	subunit,exchange
R3500	T4995	T4997	nsubjpass	cells,observed
R3501	T4996	T4995	amod	elongated,cells
R3502	T4789	T4790	punct	(,4J
R3503	T4998	T4995	acl	extending,cells
R3504	T4999	T4998	prep	from,extending
R3505	T4790	T4788	parataxis	4J,wk
R3506	T5000	T5001	det	the,region
R3507	T5001	T4999	pobj	region,from
R3508	T5002	T5001	compound	bow,region
R3509	T4791	T4790	compound	Figure,4J
R351	T880	T881	punct	[,4
R3510	T5003	T4998	prep	to,extending
R3511	T5004	T5005	det	the,capsule
R3512	T5005	T5003	pobj	capsule,to
R3513	T5006	T5005	amod	posterior,capsule
R3514	T4792	T4790	punct	),4J
R3515	T5007	T4998	prep	of,extending
R3516	T5008	T5009	compound	alphaA,BKO
R3517	T4793	T4788	cc	and,wk
R3518	T5009	T5011	compound	BKO,lenses
R3519	T5010	T5009	punct	/,BKO
R352	T881	T867	parataxis	4,exist
R3520	T5011	T5007	pobj	lenses,of
R3521	T4794	T4795	nummod	72,wk
R3522	T5012	T4997	auxpass	were,observed
R3523	T5013	T4997	punct	.,observed
R3524	T5015	T5016	prep	In,observed
R3525	T4795	T4788	conj	wk,wk
R3526	T5017	T5015	pobj	contrast,In
R3527	T4796	T4797	punct	(,shown
R3528	T5018	T5016	punct	", ",observed
R3529	T5019	T5020	amod	radial,columns
R353	T882	T881	punct	],4
R3530	T4797	T4795	parataxis	shown,wk
R3531	T5020	T5016	nsubjpass	columns,observed
R3532	T4798	T4797	nsubj	data,shown
R3533	T5021	T5020	prep	of,columns
R3534	T5022	T5023	amod	elongated,cells
R3535	T5023	T5021	pobj	cells,of
R3536	T5024	T5023	compound	fiber,cells
R3537	T5025	T5023	prep	of,cells
R3538	T4799	T4797	neg	not,shown
R3539	T5026	T5027	amod	uniform,size
R354	T883	T852	punct	.,are
R3540	T5027	T5025	pobj	size,of
R3541	T5028	T5027	cc	and,size
R3542	T4800	T4797	punct	),shown
R3543	T5029	T5027	conj	shape,size
R3544	T5030	T5023	acl	containing,cells
R3545	T5031	T5032	nmod	ball,interdigitations
R3546	T4801	T4802	amod	wild,type
R3547	T5032	T5030	dobj	interdigitations,containing
R3548	T5033	T5031	cc	and,ball
R3549	T5034	T5031	conj	socket,ball
R355	T885	T886	det	The,expression
R3550	T4802	T4781	compound	type,lenses
R3551	T5035	T5016	auxpass	were,observed
R3552	T5036	T5016	prep	in,observed
R3553	T5037	T5038	det	the,region
R3554	T4803	T4725	punct	.,observed
R3555	T5038	T5036	pobj	region,in
R3556	T5039	T5038	amod	posterior,region
R3557	T5040	T5038	amod	subcapsular,region
R3558	T4805	T4806	prep	In,observed
R3559	T5041	T5038	prep	in,region
R356	T886	T887	nsubj	expression,is
R3560	T5042	T5043	nummod	5,wk
R3561	T5043	T5044	nmod	wk,lenses
R3562	T5044	T5041	pobj	lenses,in
R3563	T4807	T4808	det	the,region
R3564	T4808	T4805	pobj	region,In
R3565	T5045	T5046	punct	(,4I
R3566	T5046	T5043	parataxis	4I,wk
R3567	T5047	T5046	compound	Figure,4I
R3568	T5048	T5046	punct	),4I
R3569	T4809	T4808	amod	anterior,region
R357	T888	T886	prep	of,expression
R3570	T5049	T5043	punct	", ",wk
R3571	T5050	T5051	nummod	46,wk
R3572	T5051	T5043	conj	wk,wk
R3573	T4810	T4808	amod	cortical,region
R3574	T5052	T5053	punct	(,4L
R3575	T5053	T5051	parataxis	4L,wk
R3576	T4811	T4808	prep	of,region
R3577	T5054	T5053	compound	Figure,4L
R3578	T5055	T5053	punct	),4L
R3579	T5056	T5051	cc	and,wk
R358	T889	T890	det	these,proteins
R3580	T4812	T4813	nummod	5,wk
R3581	T5057	T5058	nummod	72,wk
R3582	T5058	T5051	conj	wk,wk
R3583	T5059	T5060	punct	(,shown
R3584	T4813	T4814	compound	wk,lenses
R3585	T5060	T5058	parataxis	shown,wk
R3586	T5061	T5060	nsubj	data,shown
R3587	T5062	T5060	neg	not,shown
R3588	T5063	T5060	punct	),shown
R3589	T5064	T5065	amod	wild,type
R359	T890	T888	pobj	proteins,of
R3590	T5065	T5044	compound	type,lenses
R3591	T4814	T4811	pobj	lenses,of
R3592	T5066	T5016	punct	", ",observed
R3593	T5067	T5016	cc	and,observed
R3594	T5068	T5069	compound	fiber,cells
R3595	T5069	T5070	nsubjpass	cells,traced
R3596	T4815	T4816	compound	alphaA,BKO
R3597	T5070	T5016	conj	traced,observed
R3598	T5071	T5069	prep	in,cells
R3599	T5072	T5071	pobj	contact,in
R36	T173	T174	compound	animal,models
R360	T891	T890	nummod	two,proteins
R3600	T4816	T4814	compound	BKO,lenses
R3601	T5073	T5072	prep	with,contact
R3602	T5074	T5075	det	the,capsule
R3603	T4817	T4816	punct	/,BKO
R3604	T5075	T5073	pobj	capsule,with
R3605	T5076	T5075	amod	posterior,capsule
R3606	T5077	T5070	aux	could,traced
R3607	T5078	T5070	auxpass	be,traced
R3608	T4818	T4806	punct	", ",observed
R3609	T5079	T5070	advmod	back,traced
R361	T892	T886	prep	in,expression
R3610	T5080	T5079	prep	to,back
R3611	T5081	T5082	det	the,region
R3612	T4819	T4820	amod	radial,columns
R3613	T5082	T5080	pobj	region,to
R3614	T5083	T5084	amod	equatorial,bow
R3615	T5084	T5082	compound	bow,region
R3616	T4820	T4806	nsubjpass	columns,observed
R3617	T5085	T5082	cc	and,region
R3618	T5086	T5087	amod	anterior,epithelium
R3619	T5087	T5082	conj	epithelium,region
R362	T893	T894	det	the,epithelium
R3620	T4821	T4820	prep	of,columns
R3621	T5088	T5016	punct	.,observed
R3622	T4822	T4821	pobj	cells,of
R3623	T4823	T4822	prep	with,cells
R3624	T4824	T4825	amod	numerous,projections
R3625	T4825	T4823	pobj	projections,with
R3626	T4826	T4827	compound	cell,surface
R3627	T4827	T4825	compound	surface,projections
R3628	T4828	T4806	auxpass	were,observed
R3629	T4829	T4830	punct	(,4B
R363	T894	T892	pobj	epithelium,in
R3630	T4830	T4806	parataxis	4B,observed
R3631	T4831	T4830	compound	Figure,4B
R3632	T4832	T4830	punct	),4B
R364	T895	T894	compound	lens,epithelium
R365	T896	T887	punct	", ",is
R366	T897	T887	advmod	however,is
R3663	T5961	T5962	nummod	One,approach
R3664	T5962	T5963	nsubj	approach,is
R3665	T5964	T5962	prep	to,approach
R3666	T5965	T5964	pcomp	resolving,to
R3667	T5966	T5965	dobj	some,resolving
R3668	T5967	T5966	prep	of,some
R3669	T5968	T5969	det	the,functions
R367	T898	T887	punct	", ",is
R3670	T5969	T5967	pobj	functions,of
R3671	T5970	T5971	advmod	in,vivo
R3672	T5971	T5969	amod	vivo,functions
R3673	T5972	T5969	prep	of,functions
R3674	T5973	T5974	compound	alpha,crystallin
R3675	T5974	T5972	pobj	crystallin,of
R3676	T5975	T5974	punct	-,crystallin
R3677	T5976	T5977	aux	to,generate
R3678	T5977	T5963	xcomp	generate,is
R3679	T5978	T5979	compound	animal,models
R368	T899	T887	neg	not,is
R3680	T5979	T5977	dobj	models,generate
R3681	T5980	T5981	advmod	where,eliminated
R3682	T5981	T5979	relcl	eliminated,models
R3683	T5982	T5981	nsubjpass	one,eliminated
R3684	T5983	T5982	cc	or,one
R3685	T5984	T5982	conj	both,one
R3686	T5985	T5982	prep	of,one
R3687	T5986	T5987	det	the,products
R3688	T5987	T5985	pobj	products,of
R3689	T5988	T5989	compound	alpha,crystallin
R369	T900	T887	acomp	uniform,is
R3690	T5989	T5987	compound	crystallin,products
R3691	T5990	T5989	punct	-,crystallin
R3692	T5991	T5987	compound	gene,products
R3693	T5992	T5981	aux	have,eliminated
R3694	T5993	T5981	auxpass	been,eliminated
R3695	T5994	T5963	punct	.,is
R3696	T5996	T5997	nsubj	Brady,demonstrated
R3697	T5998	T5999	advmod	et,al.
R3698	T5999	T5996	advmod	al.,Brady
R3699	T6000	T6001	punct	[,22
R37	T174	T172	dobj	models,generate
R370	T901	T887	prep	throughout,is
R3700	T6001	T5996	parataxis	22,Brady
R3701	T6002	T6001	punct	],22
R3702	T6003	T5997	punct	", ",demonstrated
R3703	T6004	T5997	prep	by,demonstrated
R3704	T6005	T6006	amod	targeted,disruption
R3705	T6006	T6004	pobj	disruption,by
R3706	T6007	T6006	prep	of,disruption
R3707	T6008	T6009	det	the,gene
R3708	T6009	T6007	pobj	gene,of
R3709	T6010	T6009	compound	mouse,gene
R371	T902	T903	amod	different,regions
R3710	T6011	T6009	compound	alphaA,gene
R3711	T6012	T5997	punct	", ",demonstrated
R3712	T6013	T6014	mark	that,was
R3713	T6014	T5997	ccomp	was,demonstrated
R3714	T6015	T6016	det	this,protein
R3715	T6016	T6014	nsubj	protein,was
R3716	T6017	T6014	acomp	essential,was
R3717	T6018	T6017	prep	for,essential
R3718	T6019	T6020	det	the,maintenance
R3719	T6020	T6018	pobj	maintenance,for
R372	T903	T901	pobj	regions,throughout
R3720	T6021	T6020	prep	of,maintenance
R3721	T6022	T6023	compound	lens,transparency
R3722	T6023	T6021	pobj	transparency,of
R3723	T6024	T6014	punct	", ",was
R3724	T6025	T6026	advmod	possibly,by
R3725	T6026	T6014	prep	by,was
R3726	T6027	T6026	pcomp	maintaining,by
R3727	T6028	T6029	det	the,solubility
R3728	T6029	T6027	dobj	solubility,maintaining
R3729	T6030	T6029	prep	of,solubility
R373	T904	T903	prep	of,regions
R3730	T6031	T6030	pobj	alphaB,of
R3731	T6032	T6031	punct	", ",alphaB
R3732	T6033	T6031	cc	or,alphaB
R3733	T6034	T6035	amod	associated,proteins
R3734	T6035	T6031	conj	proteins,alphaB
R3735	T6036	T6029	punct	", ",solubility
R3736	T6037	T6029	prep	in,solubility
R3737	T6038	T6039	det	the,lens
R3738	T6039	T6037	pobj	lens,in
R3739	T6040	T5997	punct	.,demonstrated
R374	T905	T906	det	the,epithelium
R3740	T6042	T6043	det	These,lenses
R3741	T6043	T6044	nsubjpass	lenses,reported
R3742	T6045	T6044	auxpass	were,reported
R3743	T6046	T6044	advmod	also,reported
R3744	T6047	T6048	aux	to,be
R3745	T6048	T6044	xcomp	be,reported
R3746	T6049	T6048	acomp	smaller,be
R3747	T6050	T6049	prep	in,smaller
R3748	T6051	T6052	amod	equatorial,dimensions
R3749	T6052	T6050	pobj	dimensions,in
R375	T906	T904	pobj	epithelium,of
R3750	T6053	T6051	cc	and,equatorial
R3751	T6054	T6051	conj	axial,equatorial
R3752	T6055	T6049	prep	than,smaller
R3753	T6056	T6057	npadvmod	age,matched
R3754	T6057	T6058	amod	matched,lens
R3755	T6058	T6055	pobj	lens,than
R3756	T6059	T6060	amod	wild,type
R3757	T6060	T6058	compound	type,lens
R3758	T6061	T6048	punct	", ",be
R3759	T6062	T6063	dep	which,was
R376	T907	T906	amod	anterior,epithelium
R3760	T6063	T6048	advcl	was,be
R3761	T6064	T6065	advmod	very,similar
R3762	T6065	T6063	acomp	similar,was
R3763	T6066	T6065	prep	to,similar
R3764	T6067	T6066	pobj	that,to
R3765	T6068	T6069	dep	which,observed
R3766	T6069	T6067	relcl	observed,that
R3767	T6070	T6069	auxpass	was,observed
R3768	T6071	T6069	prep	with,observed
R3769	T6072	T6073	det	the,lens
R377	T908	T887	punct	.,is
R3770	T6073	T6071	pobj	lens,with
R3771	T6074	T6075	compound	double,knockout
R3772	T6075	T6073	compound	knockout,lens
R3773	T6076	T6044	punct	.,reported
R3774	T6078	T6079	amod	Targeted,disruption
R3775	T6079	T6080	nsubj	disruption,resulted
R3776	T6081	T6079	prep	of,disruption
R3777	T6082	T6083	det	the,gene
R3778	T6083	T6081	pobj	gene,of
R3779	T6084	T6083	compound	mouse,gene
R378	T910	T911	compound	AlphaB,expression
R3780	T6085	T6083	compound	alphaB,gene
R3781	T6086	T6080	punct	", ",resulted
R3782	T6087	T6080	advmod	however,resulted
R3783	T6088	T6080	punct	", ",resulted
R3784	T6089	T6080	prep	in,resulted
R3785	T6090	T6089	pobj	lenses,in
R3786	T6091	T6090	amod	similar,lenses
R3787	T6092	T6091	prep	in,similar
R3788	T6093	T6092	pobj	size,in
R3789	T6094	T6091	prep	to,similar
R379	T911	T912	nsubjpass	expression,detected
R3790	T6095	T6096	amod	aged,matched
R3791	T6096	T6098	amod	matched,lens
R3792	T6097	T6096	punct	-,matched
R3793	T6098	T6094	pobj	lens,to
R3794	T6099	T6100	amod	wild,type
R3795	T6100	T6098	compound	type,lens
R3796	T6101	T6080	prep	with,resulted
R3797	T6102	T6103	det	no,cataracts
R3798	T6103	T6101	pobj	cataracts,with
R3799	T6104	T6103	acl	reported,cataracts
R38	T175	T176	advmod	where,eliminated
R380	T913	T912	auxpass	is,detected
R3800	T6105	T6106	punct	[,23
R3801	T6106	T6080	parataxis	23,resulted
R3802	T6107	T6106	punct	],23
R3803	T6108	T6080	punct	.,resulted
R3804	T6110	T6111	nsubj	This,indicates
R3805	T6112	T6113	mark	that,play
R3806	T6113	T6111	ccomp	play,indicates
R3807	T6114	T6113	nsubj	alphaA,play
R3808	T6115	T6113	aux	may,play
R3809	T6116	T6117	det	a,role
R381	T914	T912	prep	in,detected
R3810	T6117	T6113	dobj	role,play
R3811	T6118	T6117	amod	greater,role
R3812	T6119	T6117	prep	in,role
R3813	T6120	T6119	pcomp	maintaining,in
R3814	T6121	T6122	det	the,transparency
R3815	T6122	T6120	dobj	transparency,maintaining
R3816	T6123	T6122	prep	of,transparency
R3817	T6124	T6125	det	the,lens
R3818	T6125	T6123	pobj	lens,of
R3819	T6126	T6117	prep	then,role
R382	T915	T916	amod	central,epithelium
R3820	T6127	T6126	pobj	alphaB,then
R3821	T6128	T6111	punct	.,indicates
R3822	T6130	T6131	prep	In,compensate
R3823	T6132	T6133	det	the,mice
R3824	T6133	T6130	pobj	mice,In
R3825	T6134	T6135	amod	single,knockout
R3826	T6135	T6133	compound	knockout,mice
R3827	T6136	T6137	compound	alpha,crystallin
R3828	T6137	T6135	compound	crystallin,knockout
R3829	T6138	T6137	punct	-,crystallin
R383	T916	T914	pobj	epithelium,in
R3830	T6139	T6131	punct	", ",compensate
R3831	T6140	T6141	det	the,crystallin
R3832	T6141	T6131	nsubj	crystallin,compensate
R3833	T6142	T6141	amod	remaining,crystallin
R3834	T6143	T6141	compound	alpha,crystallin
R3835	T6144	T6141	punct	-,crystallin
R3836	T6145	T6131	aux	may,compensate
R3837	T6146	T6131	advmod	fully,compensate
R3838	T6147	T6146	cc	or,fully
R3839	T6148	T6146	conj	partially,fully
R384	T917	T912	cc	and,detected
R3840	T6149	T6131	prep	for,compensate
R3841	T6150	T6149	pobj	some,for
R3842	T6151	T6150	prep	of,some
R3843	T6152	T6153	det	the,functions
R3844	T6153	T6151	pobj	functions,of
R3845	T6154	T6153	prep	of,functions
R3846	T6155	T6156	det	the,protein
R3847	T6156	T6154	pobj	protein,of
R3848	T6157	T6156	amod	missing,protein
R3849	T6158	T6131	punct	", ",compensate
R385	T918	T912	conj	increases,detected
R3850	T6159	T6160	advmod	especially,in
R3851	T6160	T6131	prep	in,compensate
R3852	T6161	T6162	det	the,lens
R3853	T6162	T6160	pobj	lens,in
R3854	T6163	T6162	punct	", ",lens
R3855	T6164	T6165	advmod	where,expressed
R3856	T6165	T6162	relcl	expressed,lens
R3857	T6166	T6167	preconj	both,alphaA
R3858	T6167	T6165	nsubjpass	alphaA,expressed
R3859	T6168	T6167	cc	and,alphaA
R386	T919	T918	prep	from,increases
R3860	T6169	T6167	conj	alphaB,alphaA
R3861	T6170	T6165	auxpass	are,expressed
R3862	T6171	T6165	advmod	normally,expressed
R3863	T6172	T6165	prep	at,expressed
R3864	T6173	T6174	amod	high,levels
R3865	T6174	T6172	pobj	levels,at
R3866	T6175	T6131	punct	.,compensate
R3867	T6177	T6178	nsubjpass	This,supported
R3868	T6179	T6178	auxpass	was,supported
R3869	T6180	T6178	agent	by,supported
R387	T920	T921	det	the,central
R3870	T6181	T6182	det	the,observation
R3871	T6182	T6180	pobj	observation,by
R3872	T6183	T6182	amod	morphological,observation
R3873	T6184	T6182	acl	made,observation
R3874	T6185	T6184	prep	in,made
R3875	T6186	T6187	det	this,study
R3876	T6187	T6185	pobj	study,in
R3877	T6188	T6182	prep	of,observation
R3878	T6189	T6190	det	no,sutures
R3879	T6190	T6188	pobj	sutures,of
R388	T921	T919	pobj	central,from
R3880	T6191	T6190	amod	posterior,sutures
R3881	T6192	T6190	cc	or,sutures
R3882	T6193	T6194	compound	fiber,cells
R3883	T6194	T6190	conj	cells,sutures
R3884	T6195	T6190	acl	extending,sutures
R3885	T6196	T6195	prep	to,extending
R3886	T6197	T6198	det	the,capsule
R3887	T6198	T6196	pobj	capsule,to
R3888	T6199	T6198	amod	posterior,capsule
R3889	T6200	T6198	prep	of,capsule
R389	T922	T919	prep	to,from
R3890	T6201	T6202	det	the,lens
R3891	T6202	T6200	pobj	lens,of
R3892	T6203	T6190	punct	", ",sutures
R3893	T6204	T6205	advmod	ectopically,staining
R3894	T6205	T6206	amod	staining,acids
R3895	T6206	T6190	appos	acids,sutures
R3896	T6207	T6206	amod	nucleic,acids
R3897	T6208	T6206	prep	in,acids
R3898	T6209	T6210	det	the,region
R3899	T6210	T6208	pobj	region,in
R39	T176	T174	relcl	eliminated,models
R390	T923	T922	pobj	elongation,to
R3900	T6211	T6210	amod	posterior,region
R3901	T6212	T6210	amod	subcapsular,region
R3902	T6213	T6210	prep	of,region
R3903	T6214	T6215	nummod	5,wk
R3904	T6215	T6213	pobj	wk,of
R3905	T6216	T6210	cc	and,region
R3906	T6217	T6218	amod	anterior,cortex
R3907	T6218	T6210	conj	cortex,region
R3908	T6219	T6218	amod	subcapsular,cortex
R3909	T6220	T6218	prep	of,cortex
R391	T924	T919	pobj	zones,from
R3910	T6221	T6222	nummod	54,wk
R3911	T6222	T6220	pobj	wk,of
R3912	T6223	T6190	punct	", ",sutures
R3913	T6224	T6225	amod	gross,differences
R3914	T6225	T6190	appos	differences,sutures
R3915	T6226	T6225	amod	morphological,differences
R3916	T6227	T6225	prep	in,differences
R3917	T6228	T6229	det	the,regions
R3918	T6229	T6227	pobj	regions,in
R3919	T6230	T6231	amod	equatorial,bow
R392	T925	T919	punct	", ",from
R3920	T6231	T6229	amod	bow,regions
R3921	T6232	T6231	punct	/,bow
R3922	T6233	T6229	punct	", ",regions
R3923	T6234	T6229	amod	posterior,regions
R3924	T6235	T6234	cc	and,posterior
R3925	T6236	T6234	conj	anterior,posterior
R3926	T6237	T6229	prep	of,regions
R3927	T6238	T6237	pobj	lenses,of
R3928	T6239	T6238	prep	from,lenses
R3929	T6240	T6241	compound	alphaA,BKO
R393	T926	T927	advmod	where,differentiate
R3930	T6241	T6243	compound	BKO,mice
R3931	T6242	T6241	punct	/,BKO
R3932	T6243	T6239	pobj	mice,from
R3933	T6244	T6245	mark	as,compared
R3934	T6245	T6225	advcl	compared,differences
R3935	T6246	T6245	prep	to,compared
R3936	T6247	T6248	amod	wild,mice
R3937	T6248	T6246	pobj	mice,to
R3938	T6249	T6178	punct	.,supported
R3939	T6251	T6252	nsubjpass	None,reported
R394	T927	T919	pcomp	differentiate,from
R3940	T6253	T6251	prep	of,None
R3941	T6254	T6255	det	these,differences
R3942	T6255	T6253	pobj	differences,of
R3943	T6256	T6255	amod	morphological,differences
R3944	T6257	T6252	aux	have,reported
R3945	T6258	T6252	auxpass	been,reported
R3946	T6259	T6252	prep	in,reported
R3947	T6260	T6261	det	the,mice
R3948	T6261	T6259	pobj	mice,in
R3949	T6262	T6263	amod	single,knockout
R395	T928	T929	amod	epithelial,cells
R3950	T6263	T6261	compound	knockout,mice
R3951	T6264	T6263	nmod	alphaA,knockout
R3952	T6265	T6264	cc	or,alphaA
R3953	T6266	T6264	conj	alphaB,alphaA
R3954	T6267	T6252	punct	.,reported
R3955	T6269	T6270	nsubjpass	It,noted
R3956	T6271	T6270	aux	must,noted
R3957	T6272	T6270	auxpass	be,noted
R3958	T6273	T6270	punct	", ",noted
R3959	T6274	T6270	advmod	however,noted
R396	T929	T927	nsubj	cells,differentiate
R3960	T6275	T6270	punct	", ",noted
R3961	T6276	T6277	mark	that,lack
R3962	T6277	T6270	ccomp	lack,noted
R3963	T6278	T6279	det	the,mice
R3964	T6279	T6277	nsubj	mice,lack
R3965	T6280	T6281	compound	alphaA,BKO
R3966	T6281	T6279	compound	BKO,mice
R3967	T6282	T6281	punct	/,BKO
R3968	T6283	T6277	advmod	also,lack
R3969	T6284	T6285	det	the,product
R397	T930	T927	prep	into,differentiate
R3970	T6285	T6277	dobj	product,lack
R3971	T6286	T6287	compound	HSPB2,gene
R3972	T6287	T6285	compound	gene,product
R3973	T6288	T6289	punct	[,23
R3974	T6289	T6277	parataxis	23,lack
R3975	T6290	T6289	punct	],23
R3976	T6291	T6277	cc	and,lack
R3977	T6292	T6293	det	the,contribution
R3978	T6293	T6294	nsubjpass	contribution,overlooked
R3979	T6294	T6277	conj	overlooked,lack
R398	T931	T932	compound	fiber,cells
R3980	T6295	T6293	prep	of,contribution
R3981	T6296	T6297	det	this,protein
R3982	T6297	T6295	pobj	protein,of
R3983	T6298	T6293	prep	to,contribution
R3984	T6299	T6300	amod	normal,morphology
R3985	T6300	T6298	pobj	morphology,to
R3986	T6301	T6300	compound	lens,morphology
R3987	T6302	T6300	cc	and,morphology
R3988	T6303	T6300	conj	functions,morphology
R3989	T6304	T6294	aux	should,overlooked
R399	T932	T930	pobj	cells,into
R3990	T6305	T6294	neg	not,overlooked
R3991	T6306	T6294	auxpass	be,overlooked
R3992	T6307	T6270	punct	.,noted
R3993	T6309	T6310	amod	Future,studies
R3994	T6310	T6311	nsubj	studies,address
R3995	T6312	T6311	aux	should,address
R3996	T6313	T6314	det	the,functions
R3997	T6314	T6311	dobj	functions,address
R3998	T6315	T6314	amod	possible,functions
R3999	T6316	T6314	prep	of,functions
R40	T177	T176	nsubjpass	one,eliminated
R400	T933	T912	punct	.,detected
R4000	T6317	T6316	pobj	HSPB2,of
R4001	T6318	T6314	prep	in,functions
R4002	T6319	T6320	amod	normal,lens
R4003	T6320	T6318	pobj	lens,in
R4004	T6321	T6311	punct	.,address
R4005	T6323	T6324	det	The,results
R4006	T6324	T6325	nsubj	results,support
R4007	T6326	T6324	prep	of,results
R4008	T6327	T6328	det	the,study
R4009	T6328	T6326	pobj	study,of
R401	T935	T936	nsubjpass	AlphaA,detected
R4010	T6329	T6328	amod	current,study
R4011	T6330	T6331	det	the,hypothesis
R4012	T6331	T6325	dobj	hypothesis,support
R4013	T6332	T6333	mark	that,plays
R4014	T6333	T6331	acl	plays,hypothesis
R4015	T6334	T6335	compound	alpha,crystallin
R4016	T6335	T6333	nsubj	crystallin,plays
R4017	T6336	T6335	punct	-,crystallin
R4018	T6337	T6338	det	an,role
R4019	T6338	T6333	dobj	role,plays
R402	T937	T936	punct	", ",detected
R4020	T6339	T6338	amod	active,role
R4021	T6340	T6333	prep	in,plays
R4022	T6341	T6342	det	the,differentiation
R4023	T6342	T6340	pobj	differentiation,in
R4024	T6343	T6342	cc	and,differentiation
R4025	T6344	T6342	conj	growth,differentiation
R4026	T6345	T6342	prep	of,differentiation
R4027	T6346	T6347	compound	lens,fiber
R4028	T6347	T6348	compound	fiber,cells
R4029	T6348	T6345	pobj	cells,of
R403	T938	T936	advmod	however,detected
R4030	T6349	T6325	punct	.,support
R4031	T6351	T6352	amod	Normal,differentiation
R4032	T6352	T6353	nsubj	differentiation,consists
R4033	T6354	T6352	prep	of,differentiation
R4034	T6355	T6356	compound	lens,fiber
R4035	T6356	T6357	compound	fiber,cells
R4036	T6357	T6354	pobj	cells,of
R4037	T6358	T6353	prep	of,consists
R4038	T6359	T6360	det	a,progression
R4039	T6360	T6358	pobj	progression,of
R404	T939	T936	punct	", ",detected
R4040	T6361	T6360	prep	from,progression
R4041	T6362	T6363	det	a,cell
R4042	T6363	T6361	pobj	cell,from
R4043	T6364	T6363	amod	simple,cell
R4044	T6365	T6363	amod	cuboidal,cell
R4045	T6366	T6363	amod	epithelial,cell
R4046	T6367	T6363	punct	", ",cell
R4047	T6368	T6363	acl	containing,cell
R4048	T6369	T6370	det	a,nucleus
R4049	T6370	T6368	dobj	nucleus,containing
R405	T940	T936	auxpass	is,detected
R4050	T6371	T6370	cc	and,nucleus
R4051	T6372	T6373	det	a,numbers
R4052	T6373	T6370	conj	numbers,nucleus
R4053	T6374	T6373	amod	minimal,numbers
R4054	T6375	T6373	prep	of,numbers
R4055	T6376	T6375	pobj	organelles,of
R4056	T6377	T6360	punct	", ",progression
R4057	T6378	T6360	prep	to,progression
R4058	T6379	T6380	det	a,layer
R4059	T6380	T6378	pobj	layer,to
R406	T941	T936	neg	not,detected
R4060	T6381	T6380	amod	stratified,layer
R4061	T6382	T6380	prep	of,layer
R4062	T6383	T6384	amod	elongated,cells
R4063	T6384	T6382	pobj	cells,of
R4064	T6385	T6386	npadvmod	fiber,like
R4065	T6386	T6384	amod	like,cells
R4066	T6387	T6386	punct	-,like
R4067	T6388	T6384	punct	", ",cells
R4068	T6389	T6384	amod	devoid,cells
R4069	T6390	T6389	prep	of,devoid
R407	T942	T936	prep	in,detected
R4070	T6391	T6390	pobj	nuclei,of
R4071	T6392	T6391	cc	and,nuclei
R4072	T6393	T6391	conj	organelles,nuclei
R4073	T6394	T6353	punct	.,consists
R4074	T6396	T6397	nsubj	Differentiation,occurs
R4075	T6398	T6396	prep	of,Differentiation
R4076	T6399	T6400	amod	epithelial,cells
R4077	T6400	T6398	pobj	cells,of
R4078	T6401	T6397	prep	in,occurs
R4079	T6402	T6403	det	the,region
R408	T943	T944	det	the,epithelium
R4080	T6403	T6401	pobj	region,in
R4081	T6404	T6405	amod	equatorial,bow
R4082	T6405	T6403	amod	bow,region
R4083	T6406	T6405	punct	/,bow
R4084	T6407	T6403	prep	of,region
R4085	T6408	T6409	det	the,lens
R4086	T6409	T6407	pobj	lens,of
R4087	T6410	T6403	punct	", ",region
R4088	T6411	T6412	advmod	where,begin
R4089	T6412	T6403	relcl	begin,region
R409	T944	T942	pobj	epithelium,in
R4090	T6413	T6414	amod	epithelial,cells
R4091	T6414	T6412	nsubj	cells,begin
R4092	T6415	T6416	aux	to,elongate
R4093	T6416	T6412	xcomp	elongate,begin
R4094	T6417	T6416	cc	and,elongate
R4095	T6418	T6416	conj	differentiate,elongate
R4096	T6419	T6418	prep	into,differentiate
R4097	T6420	T6421	compound	fiber,cells
R4098	T6421	T6419	pobj	cells,into
R4099	T6422	T6421	prep	of,cells
R41	T178	T177	cc	or,one
R410	T945	T944	amod	central,epithelium
R4100	T6423	T6424	amod	uniform,shape
R4101	T6424	T6422	pobj	shape,of
R4102	T6425	T6424	amod	cellular,shape
R4103	T6426	T6421	punct	", ",cells
R4104	T6427	T6421	acl	arranged,cells
R4105	T6428	T6427	prep	in,arranged
R4106	T6429	T6430	amod	radial,columns
R4107	T6430	T6428	pobj	columns,in
R4108	T6431	T6430	prep	of,columns
R4109	T6432	T6431	pobj	cells,of
R411	T946	T936	punct	.,detected
R4110	T6433	T6430	acl	extending,columns
R4111	T6434	T6433	prep	from,extending
R4112	T6435	T6436	det	the,epithelium
R4113	T6436	T6434	pobj	epithelium,from
R4114	T6437	T6436	amod	anterior,epithelium
R4115	T6438	T6433	prep	to,extending
R4116	T6439	T6440	det	the,capsule
R4117	T6440	T6438	pobj	capsule,to
R4118	T6441	T6440	amod	posterior,capsule
R4119	T6442	T6397	punct	.,occurs
R412	T948	T949	det	The,proportion
R4120	T6444	T6445	det	This,process
R4121	T6445	T6446	nsubj	process,appear
R4122	T6447	T6446	aux	did,appear
R4123	T6448	T6446	neg	not,appear
R4124	T6449	T6450	aux	to,proceeded
R4125	T6450	T6446	xcomp	proceeded,appear
R4126	T6451	T6450	aux	have,proceeded
R4127	T6452	T6450	advmod	normally,proceeded
R4128	T6453	T6450	prep	in,proceeded
R4129	T6454	T6453	pobj	lenses,in
R413	T949	T951	nsubj	proportion,changes
R4130	T6455	T6454	acl	lacking,lenses
R4131	T6456	T6455	dobj	alphaA,lacking
R4132	T6457	T6456	cc	and,alphaA
R4133	T6458	T6456	conj	alphaB,alphaA
R4134	T6459	T6446	punct	.,appear
R4135	T6461	T6462	det	The,observations
R4136	T6462	T6464	nsubj	observations,demonstrate
R4137	T6463	T6462	amod	morphological,observations
R4138	T6465	T6462	acl	presented,observations
R4139	T6466	T6465	prep	in,presented
R414	T950	T949	amod	relative,proportion
R4140	T6467	T6468	det	this,study
R4141	T6468	T6466	pobj	study,in
R4142	T6469	T6470	mark	that,fail
R4143	T6470	T6464	ccomp	fail,demonstrate
R4144	T6471	T6472	compound	fiber,cells
R4145	T6472	T6470	nsubj	cells,fail
R4146	T6473	T6472	prep	in,cells
R4147	T6474	T6473	pobj	lenses,in
R4148	T6475	T6474	acl	lacking,lenses
R4149	T6476	T6475	dobj	alphaA,lacking
R415	T952	T949	prep	of,proportion
R4150	T6477	T6476	cc	and,alphaA
R4151	T6478	T6476	conj	alphaB,alphaA
R4152	T6479	T6480	aux	to,elongate
R4153	T6480	T6470	xcomp	elongate,fail
R4154	T6481	T6480	advmod	symmetrically,elongate
R4155	T6482	T6480	prep	from,elongate
R4156	T6483	T6484	det	the,region
R4157	T6484	T6482	pobj	region,from
R4158	T6485	T6484	compound	bow,region
R4159	T6486	T6470	cc	and,fail
R416	T953	T952	pobj	alphaA,of
R4160	T6487	T6488	advmod	therefore,establish
R4161	T6488	T6470	conj	establish,fail
R4162	T6489	T6488	aux	do,establish
R4163	T6490	T6488	neg	not,establish
R4164	T6491	T6492	det	the,conformation
R4165	T6492	T6488	dobj	conformation,establish
R4166	T6493	T6492	amod	typical,conformation
R4167	T6494	T6492	punct	"""",conformation
R4168	T6495	T6496	nmod	onion,skin
R4169	T6496	T6492	nmod	skin,conformation
R417	T954	T953	cc	and,alphaA
R4170	T6497	T6492	punct	"""",conformation
R4171	T6498	T6499	prep	in,extend
R4172	T6499	T6492	relcl	extend,conformation
R4173	T6500	T6498	pobj	which,in
R4174	T6501	T6499	nsubj	cells,extend
R4175	T6502	T6499	prep	from,extend
R4176	T6503	T6504	det	the,epithelium
R4177	T6504	T6502	pobj	epithelium,from
R4178	T6505	T6504	amod	anterior,epithelium
R4179	T6506	T6499	prep	to,extend
R418	T955	T953	conj	alphaB,alphaA
R4180	T6507	T6508	det	the,capsule
R4181	T6508	T6506	pobj	capsule,to
R4182	T6509	T6508	amod	posterior,capsule
R4183	T6510	T6464	punct	.,demonstrate
R4184	T6512	T6513	advmod	Additionally,was
R4185	T6514	T6513	punct	", ",was
R4186	T6515	T6513	prep	in,was
R4187	T6516	T6515	pobj	lenses,in
R4188	T6517	T6516	prep	from,lenses
R4189	T6518	T6519	nummod	54,wk
R419	T956	T951	prep	from,changes
R4190	T6519	T6520	compound	wk,lenses
R4191	T6520	T6517	pobj	lenses,from
R4192	T6521	T6522	compound	alphaA,BKO
R4193	T6522	T6520	compound	BKO,lenses
R4194	T6523	T6522	punct	/,BKO
R4195	T6524	T6513	punct	", ",was
R4196	T6525	T6513	expl	there,was
R4197	T6526	T6527	det	a,persistence
R4198	T6527	T6513	attr	persistence,was
R4199	T6528	T6527	prep	of,persistence
R42	T179	T177	conj	both,one
R420	T957	T958	det	a,ratio
R4200	T6529	T6530	compound	cell,nuclei
R4201	T6530	T6528	pobj	nuclei,of
R4202	T6531	T6527	prep	in,persistence
R4203	T6532	T6533	amod	deeper,regions
R4204	T6533	T6531	pobj	regions,in
R4205	T6534	T6533	amod	cortical,regions
R4206	T6535	T6513	punct	", ",was
R4207	T6536	T6513	cc	and,was
R4208	T6537	T6538	amod	ectopic,nuclei
R4209	T6538	T6540	nsubj	nuclei,were
R421	T958	T956	pobj	ratio,from
R4210	T6539	T6538	compound	cell,nuclei
R4211	T6540	T6513	conj	were,was
R4212	T6541	T6540	acomp	present,were
R4213	T6542	T6540	prep	in,were
R4214	T6543	T6544	amod	large,numbers
R4215	T6544	T6542	pobj	numbers,in
R4216	T6545	T6540	prep	in,were
R4217	T6546	T6547	det	the,cortex
R4218	T6547	T6545	pobj	cortex,in
R4219	T6548	T6547	amod	anterior,cortex
R422	T959	T958	amod	molar,ratio
R4220	T6549	T6547	amod	central,cortex
R4221	T6550	T6513	punct	.,was
R4222	T6552	T6553	prep	At,were
R4223	T6554	T6555	nummod	5,wks
R4224	T6555	T6552	pobj	wks,At
R4225	T6556	T6555	prep	of,wks
R4226	T6557	T6556	pobj	age,of
R4227	T6558	T6559	compound	cell,nuclei
R4228	T6559	T6553	nsubj	nuclei,were
R4229	T6560	T6553	acomp	present,were
R423	T960	T958	prep	of,ratio
R4230	T6561	T6553	punct	", ",were
R4231	T6562	T6553	advcl	adjacent,were
R4232	T6563	T6562	prep	to,adjacent
R4233	T6564	T6565	det	the,capsule
R4234	T6565	T6563	pobj	capsule,to
R4235	T6566	T6565	amod	posterior,capsule
R4236	T6567	T6553	punct	.,were
R4237	T6569	T6570	det	These,observations
R4238	T6570	T6572	nsubj	observations,are
R4239	T6571	T6570	amod	morphological,observations
R424	T961	T960	pobj	1,of
R4240	T6573	T6572	acomp	consistent,are
R4241	T6574	T6573	prep	with,consistent
R4242	T6575	T6576	det	a,defect
R4243	T6576	T6574	pobj	defect,with
R4244	T6577	T6576	prep	in,defect
R4245	T6578	T6579	det	the,pathway
R4246	T6579	T6577	pobj	pathway,in
R4247	T6580	T6579	amod	normal,pathway
R4248	T6581	T6579	compound	differentiation,pathway
R4249	T6582	T6579	prep	of,pathway
R425	T962	T963	punct	:,3
R4250	T6583	T6584	nmod	lens,cells
R4251	T6584	T6582	pobj	cells,of
R4252	T6585	T6584	amod	epithelial,cells
R4253	T6586	T6579	prep	into,pathway
R4254	T6587	T6588	compound	fiber,cells
R4255	T6588	T6586	pobj	cells,into
R4256	T6589	T6572	punct	.,are
R4257	T6591	T6592	nsubj	It,is
R4258	T6593	T6592	acomp	unlikely,is
R4259	T6594	T6595	mark	that,result
R426	T963	T961	prep	3,1
R4260	T6595	T6592	ccomp	result,is
R4261	T6596	T6597	det	these,alterations
R4262	T6597	T6595	nsubj	alterations,result
R4263	T6598	T6597	prep	in,alterations
R4264	T6599	T6600	compound	alphaA,BKO
R4265	T6600	T6602	compound	BKO,lenses
R4266	T6601	T6600	punct	/,BKO
R4267	T6602	T6598	pobj	lenses,in
R4268	T6603	T6602	compound	mouse,lenses
R4269	T6604	T6595	prep	from,result
R427	T964	T958	prep	in,ratio
R4270	T6605	T6606	amod	increased,susceptibility
R4271	T6606	T6604	pobj	susceptibility,from
R4272	T6607	T6606	prep	of,susceptibility
R4273	T6608	T6609	det	these,lenses
R4274	T6609	T6607	pobj	lenses,of
R4275	T6610	T6606	prep	to,susceptibility
R4276	T6611	T6612	npadvmod	light,induced
R4277	T6612	T6614	amod	induced,damage
R4278	T6613	T6612	punct	-,induced
R4279	T6614	T6610	pobj	damage,to
R428	T965	T966	det	the,zones
R4280	T6615	T6606	prep	in,susceptibility
R4281	T6616	T6617	det	the,absence
R4282	T6617	T6615	pobj	absence,in
R4283	T6618	T6617	prep	of,absence
R4284	T6619	T6620	det	the,protection
R4285	T6620	T6618	pobj	protection,of
R4286	T6621	T6620	amod	molecular,protection
R4287	T6622	T6620	compound	chaperone,protection
R4288	T6623	T6620	acl	afforded,protection
R4289	T6624	T6623	agent	by,afforded
R429	T966	T964	pobj	zones,in
R4290	T6625	T6624	pobj	alphaA,by
R4291	T6626	T6625	cc	and,alphaA
R4292	T6627	T6625	conj	alphaB,alphaA
R4293	T6628	T6623	prep	in,afforded
R4294	T6629	T6630	amod	normal,lenses
R4295	T6630	T6628	pobj	lenses,in
R4296	T6631	T6592	punct	.,is
R4297	T6633	T6634	prep	With,had
R4298	T6635	T6636	det	the,time
R4299	T6636	T6633	pobj	time,With
R43	T180	T177	prep	of,one
R430	T967	T966	amod	pericentral,zones
R4300	T6637	T6636	amod	normal,time
R4301	T6638	T6636	prep	of,time
R4302	T6639	T6640	compound	eye,opening
R4303	T6640	T6638	pobj	opening,of
R4304	T6641	T6636	prep	at,time
R4305	T6642	T6643	advmod	approximately,14
R4306	T6643	T6644	nummod	14,days
R4307	T6644	T6641	pobj	days,at
R4308	T6645	T6644	prep	after,days
R4309	T6646	T6645	pobj	birth,after
R431	T968	T967	cc	and,pericentral
R4310	T6647	T6634	punct	", ",had
R4311	T6648	T6649	det	the,mice
R4312	T6649	T6634	nsubj	mice,had
R4313	T6650	T6651	nummod	5,wk
R4314	T6651	T6652	npadvmod	wk,old
R4315	T6652	T6649	amod	old,mice
R4316	T6653	T6654	poss	their,eyes
R4317	T6654	T6655	nsubj	eyes,open
R4318	T6655	T6634	ccomp	open,had
R4319	T6656	T6655	cc	and,open
R432	T969	T967	conj	germative,pericentral
R4320	T6657	T6658	nsubj	lenses,exposed
R4321	T6658	T6655	conj	exposed,open
R4322	T6659	T6658	prep	to,exposed
R4323	T6660	T6659	pobj	light,to
R4324	T6661	T6655	prep	for,open
R4325	T6662	T6663	advmod	only,3
R4326	T6663	T6665	nummod	3,weeks
R4327	T6664	T6663	advmod	about,3
R4328	T6665	T6661	pobj	weeks,for
R4329	T6666	T6655	advmod	prior,open
R433	T970	T951	prep	to,changes
R4330	T6667	T6666	prep	to,prior
R4331	T6668	T6669	amod	morphological,analysis
R4332	T6669	T6667	pobj	analysis,to
R4333	T6670	T6634	punct	.,had
R4334	T6672	T6673	advmod	Moreover,exposed
R4335	T6674	T6673	punct	", ",exposed
R4336	T6675	T6676	det	these,animals
R4337	T6676	T6673	nsubjpass	animals,exposed
R4338	T6677	T6673	aux	had,exposed
R4339	T6678	T6673	auxpass	been,exposed
R434	T971	T972	det	a,ratio
R4340	T6679	T6673	prep	to,exposed
R4341	T6680	T6681	advmod	only,lighting
R4342	T6681	T6679	pobj	lighting,to
R4343	T6682	T6683	amod	animal,facility
R4344	T6683	T6681	nmod	facility,lighting
R4345	T6684	T6681	amod	fluorescent,lighting
R4346	T6685	T6686	cc	and,protected
R4347	T6686	T6673	dep	protected,exposed
R4348	T6687	T6686	auxpass	were,protected
R4349	T6688	T6686	prep	from,protected
R435	T972	T970	pobj	ratio,to
R4350	T6689	T6690	compound	UV,light
R4351	T6690	T6688	pobj	light,from
R4352	T6691	T6686	prep	by,protected
R4353	T6692	T6693	amod	plastic,cages
R4354	T6693	T6691	pobj	cages,by
R4355	T6694	T6673	punct	.,exposed
R4356	T6696	T6697	mark	If,was
R4357	T6697	T6706	advcl	was,resided
R4358	T6698	T6697	nsubj	lack,was
R4359	T6699	T6698	prep	of,lack
R436	T973	T972	amod	molar,ratio
R4360	T6700	T6699	pobj	protection,of
R4361	T6701	T6700	prep	from,protection
R4362	T6702	T6703	npadvmod	light,induced
R4363	T6703	T6705	amod	induced,damage
R4364	T6704	T6703	punct	-,induced
R4365	T6705	T6701	pobj	damage,from
R4366	T6707	T6708	det	the,factor
R4367	T6708	T6697	attr	factor,was
R4368	T6709	T6708	amod	major,factor
R4369	T6710	T6708	acl	affecting,factor
R437	T974	T972	prep	of,ratio
R4370	T6711	T6712	det	the,changes
R4371	T6712	T6710	dobj	changes,affecting
R4372	T6713	T6710	prep	in,affecting
R4373	T6714	T6715	det	these,lenses
R4374	T6715	T6713	pobj	lenses,in
R4375	T6716	T6706	punct	", ",resided
R4376	T6717	T6706	advmod	then,resided
R4377	T6718	T6719	det	the,bulk
R4378	T6719	T6706	nsubj	bulk,resided
R4379	T6720	T6719	prep	of,bulk
R438	T975	T974	pobj	3,of
R4380	T6721	T6722	det	the,damage
R4381	T6722	T6720	pobj	damage,of
R4382	T6723	T6706	aux	should,resided
R4383	T6724	T6706	aux	have,resided
R4384	T6725	T6706	prep	along,resided
R4385	T6726	T6727	det	the,axis
R4386	T6727	T6725	pobj	axis,along
R4387	T6728	T6727	amod	visual,axis
R4388	T6729	T6706	punct	", ",resided
R4389	T6730	T6731	advmod	particularly,in
R439	T976	T977	punct	:,1
R4390	T6731	T6706	prep	in,resided
R4391	T6732	T6733	det	the,epithelium
R4392	T6733	T6731	pobj	epithelium,in
R4393	T6734	T6733	amod	central,epithelium
R4394	T6735	T6733	amod	anterior,epithelium
R4395	T6736	T6733	cc	and,epithelium
R4396	T6737	T6738	amod	subcapsular,cortex
R4397	T6738	T6733	conj	cortex,epithelium
R4398	T6739	T6706	prep	in,resided
R4399	T6740	T6741	det	the,lenses
R44	T181	T182	det	the,products
R440	T977	T975	prep	1,3
R4400	T6741	T6739	pobj	lenses,in
R4401	T6742	T6743	nummod	5,wk
R4402	T6743	T6741	npadvmod	wk,lenses
R4403	T6744	T6706	punct	", ",resided
R4404	T6745	T6706	cc	but,resided
R4405	T6746	T6747	nsubj	this,was
R4406	T6747	T6706	conj	was,resided
R4407	T6748	T6747	neg	not,was
R4408	T6749	T6750	det	the,case
R4409	T6750	T6747	attr	case,was
R441	T978	T972	prep	in,ratio
R4410	T6751	T6747	punct	.,was
R4411	T6753	T6754	prep	In,were
R4412	T6755	T6756	det	these,lenses
R4413	T6756	T6753	pobj	lenses,In
R4414	T6757	T6754	punct	", ",were
R4415	T6758	T6759	amod	gross,changes
R4416	T6759	T6754	nsubj	changes,were
R4417	T6760	T6759	amod	morphological,changes
R4418	T6761	T6754	acomp	apparent,were
R4419	T6762	T6754	prep	in,were
R442	T979	T980	det	the,zone
R4420	T6763	T6764	det	the,regions
R4421	T6764	T6762	pobj	regions,in
R4422	T6765	T6764	amod	equatorial,regions
R4423	T6766	T6765	cc	and,equatorial
R4424	T6767	T6765	conj	posterior,equatorial
R4425	T6768	T6764	amod	subcapsular,regions
R4426	T6769	T6754	punct	.,were
R4427	T6771	T6772	det	These,changes
R4428	T6772	T6773	nsubj	changes,included
R4429	T6774	T6775	amod	posterior,staining
R443	T980	T978	pobj	zone,in
R4430	T6775	T6773	dobj	staining,included
R4431	T6776	T6775	amod	subcapsular,staining
R4432	T6777	T6778	amod	nucleic,acid
R4433	T6778	T6775	compound	acid,staining
R4434	T6779	T6775	punct	", ",staining
R4435	T6780	T6775	conj	absence,staining
R4436	T6781	T6780	prep	of,absence
R4437	T6782	T6783	amod	posterior,sutures
R4438	T6783	T6781	pobj	sutures,of
R4439	T6784	T6780	punct	", ",absence
R444	T981	T980	compound	elongation,zone
R4440	T6785	T6780	cc	and,absence
R4441	T6786	T6787	amod	small,cells
R4442	T6787	T6780	conj	cells,absence
R4443	T6788	T6789	advmod	irregularly,shaped
R4444	T6789	T6787	amod	shaped,cells
R4445	T6790	T6787	punct	", ",cells
R4446	T6791	T6792	neg	not,arranged
R4447	T6792	T6787	acl	arranged,cells
R4448	T6793	T6792	prep	in,arranged
R4449	T6794	T6795	det	any,pattern
R445	T982	T980	cc	and,zone
R4450	T6795	T6793	pobj	pattern,in
R4451	T6796	T6795	amod	discernable,pattern
R4452	T6797	T6792	punct	", ",arranged
R4453	T6798	T6792	prep	in,arranged
R4454	T6799	T6800	det	the,region
R4455	T6800	T6798	pobj	region,in
R4456	T6801	T6802	amod	equatorial,bow
R4457	T6802	T6800	amod	bow,region
R4458	T6803	T6802	punct	/,bow
R4459	T6804	T6773	punct	.,included
R446	T983	T984	compound	fiber,cells
R4460	T6806	T6807	amod	Systemic,stress
R4461	T6807	T6808	compound	stress,factors
R4462	T6808	T6809	nsubj	factors,explain
R4463	T6810	T6808	acl	crossing,factors
R4464	T6811	T6812	det	the,barrier
R4465	T6812	T6810	dobj	barrier,crossing
R4466	T6813	T6814	npadvmod	blood,aqueous
R4467	T6814	T6812	amod	aqueous,barrier
R4468	T6815	T6814	punct	/,aqueous
R4469	T6816	T6809	aux	might,explain
R447	T984	T980	conj	cells,zone
R4470	T6817	T6818	det	some,changes
R4471	T6818	T6809	dobj	changes,explain
R4472	T6819	T6818	amod	morphological,changes
R4473	T6820	T6818	prep	at,changes
R4474	T6821	T6822	det	the,region
R4475	T6822	T6820	pobj	region,at
R4476	T6823	T6822	amod	equatorial,region
R4477	T6824	T6809	punct	", ",explain
R4478	T6825	T6809	cc	but,explain
R4479	T6826	T6827	nsubj	this,explain
R448	T985	T986	punct	[,3
R4480	T6827	T6809	conj	explain,explain
R4481	T6828	T6827	aux	would,explain
R4482	T6829	T6827	neg	not,explain
R4483	T6830	T6831	amod	nucleic,acid
R4484	T6831	T6832	compound	acid,staining
R4485	T6832	T6827	dobj	staining,explain
R4486	T6833	T6832	prep	in,staining
R4487	T6834	T6835	det	the,region
R4488	T6835	T6833	pobj	region,in
R4489	T6836	T6835	amod	posterior,region
R449	T986	T951	parataxis	3,changes
R4490	T6837	T6835	amod	subcapsular,region
R4491	T6838	T6827	punct	.,explain
R4492	T6873	T6861	conj	cells,cells
R4493	T6840	T6841	prep	In,is
R4494	T6874	T6873	compound	fiber,cells
R4495	T6875	T6873	acl	failing,cells
R4496	T6842	T6843	det	the,lenses
R4497	T6876	T6877	aux	to,differentiate
R4498	T6877	T6875	xcomp	differentiate,failing
R4499	T6878	T6877	advmod	fully,differentiate
R45	T182	T180	pobj	products,of
R450	T987	T986	nummod	2,3
R4500	T6843	T6840	pobj	lenses,In
R4501	T6879	T6877	prep	by,differentiate
R4502	T6880	T6881	nummod	5,wks
R4503	T6881	T6879	pobj	wks,by
R4504	T6844	T6845	nummod	5,wk
R4505	T6882	T6881	prep	of,wks
R4506	T6883	T6882	pobj	age,of
R4507	T6845	T6843	compound	wk,lenses
R4508	T6884	T6841	punct	.,is
R4509	T6886	T6887	det	These,possibilities
R451	T988	T986	punct	",",3
R4510	T6846	T6847	compound	alphaA,BKO
R4511	T6887	T6889	nsubjpass	possibilities,differentiated
R4512	T6888	T6887	nummod	two,possibilities
R4513	T6847	T6843	compound	BKO,lenses
R4514	T6890	T6889	aux	could,differentiated
R4515	T6891	T6889	neg	not,differentiated
R4516	T6848	T6847	punct	/,BKO
R4517	T6892	T6889	auxpass	be,differentiated
R4518	T6893	T6889	prep	in,differentiated
R4519	T6894	T6895	det	the,methods
R452	T989	T986	punct	],3
R4520	T6895	T6893	pobj	methods,in
R4521	T6896	T6895	acl	employed,methods
R4522	T6849	T6841	punct	", ",is
R4523	T6897	T6889	punct	", ",differentiated
R4524	T6898	T6889	cc	and,differentiated
R4525	T6899	T6889	conj	were,differentiated
R4526	T6900	T6899	prep	beyond,were
R4527	T6850	T6851	amod	nucleic,acid
R4528	T6901	T6902	det	the,objectives
R4529	T6902	T6900	pobj	objectives,beyond
R453	T990	T951	punct	.,changes
R4530	T6851	T6852	compound	acid,staining
R4531	T6903	T6902	prep	of,objectives
R4532	T6904	T6905	det	the,study
R4533	T6852	T6841	nsubj	staining,is
R4534	T6905	T6903	pobj	study,of
R4535	T6906	T6905	amod	current,study
R4536	T6907	T6889	punct	.,differentiated
R4537	T6909	T6910	amod	Future,studies
R4538	T6853	T6852	prep	in,staining
R4539	T6910	T6911	nsubjpass	studies,designed
R454	T992	T993	det	These,differences
R4540	T6854	T6855	det	the,region
R4541	T6912	T6911	aux	are,designed
R4542	T6913	T6911	auxpass	being,designed
R4543	T6914	T6915	aux	to,address
R4544	T6855	T6853	pobj	region,in
R4545	T6915	T6911	advcl	address,designed
R4546	T6916	T6917	dep	which,explain
R4547	T6856	T6855	amod	posterior,region
R4548	T6857	T6855	amod	subcapsular,region
R4549	T6917	T6915	ccomp	explain,address
R455	T993	T994	nsubj	differences,suggest
R4550	T6918	T6916	prep	of,which
R4551	T6858	T6841	acomp	consistent,is
R4552	T6919	T6920	det	these,processes
R4553	T6920	T6918	pobj	processes,of
R4554	T6921	T6920	nummod	two,processes
R4555	T6859	T6858	prep	with,consistent
R4556	T6922	T6917	aux	might,explain
R4557	T6923	T6924	amod	nucleic,acid
R4558	T6860	T6861	preconj	either,cells
R4559	T6924	T6925	compound	acid,staining
R456	T995	T993	prep	in,differences
R4560	T6925	T6917	dobj	staining,explain
R4561	T6926	T6925	prep	in,staining
R4562	T6861	T6859	pobj	cells,with
R4563	T6927	T6928	det	the,region
R4564	T6928	T6926	pobj	region,in
R4565	T6862	T6861	amod	anterior,cells
R4566	T6929	T6928	amod	posterior,region
R4567	T6930	T6928	amod	subcapsular,region
R4568	T6931	T6911	punct	.,designed
R4569	T6863	T6861	amod	epithelial,cells
R457	T996	T997	det	the,proportions
R4570	T6933	T6934	nsubjpass	Staining,observed
R4571	T6864	T6861	acl	migrating,cells
R4572	T6935	T6933	prep	in,Staining
R4573	T6936	T6937	det	this,region
R4574	T6937	T6935	pobj	region,in
R4575	T6938	T6934	auxpass	was,observed
R4576	T6865	T6864	advmod	aberrantly,migrating
R4577	T6939	T6934	neg	not,observed
R4578	T6940	T6934	prep	in,observed
R4579	T6941	T6942	amod	older,lenses
R458	T997	T995	pobj	proportions,in
R4580	T6866	T6864	prep	to,migrating
R4581	T6942	T6940	pobj	lenses,in
R4582	T6943	T6944	compound	alphaA,BKO
R4583	T6944	T6942	compound	BKO,lenses
R4584	T6867	T6868	det	the,pole
R4585	T6945	T6944	punct	/,BKO
R4586	T6946	T6934	punct	", ",observed
R4587	T6947	T6934	advcl	suggesting,observed
R4588	T6868	T6866	pobj	pole,to
R4589	T6948	T6949	mark	that,was
R459	T998	T997	amod	relative,proportions
R4590	T6949	T6947	ccomp	was,suggesting
R4591	T6869	T6868	amod	posterior,pole
R4592	T6950	T6951	det	this,pattern
R4593	T6951	T6949	nsubj	pattern,was
R4594	T6952	T6949	acomp	transient,was
R4595	T6870	T6861	punct	", ",cells
R4596	T6953	T6934	punct	.,observed
R4597	T6955	T6956	det	The,fate
R4598	T6871	T6861	cc	or,cells
R4599	T6956	T6957	nsubjpass	fate,known
R46	T183	T184	compound	alpha,crystallin
R460	T999	T997	prep	of,proportions
R4600	T6958	T6956	prep	of,fate
R4601	T6872	T6873	amod	primary,cells
R4602	T6959	T6960	det	the,cells
R4603	T6960	T6958	pobj	cells,of
R4604	T6961	T6962	amod	nucleic,acid
R4605	T6979	T6957	cc	nor,known
R4606	T6962	T6963	npadvmod	acid,containing
R4607	T6963	T6960	amod	containing,cells
R4608	T6980	T6957	conj	is,known
R4609	T6964	T6963	punct	-,containing
R461	T1000	T999	pobj	alphaA,of
R4610	T6965	T6960	prep	in,cells
R4611	T6966	T6967	det	the,region
R4612	T6981	T6982	det	the,morphology
R4613	T6967	T6965	pobj	region,in
R4614	T6968	T6967	amod	posterior,region
R4615	T6969	T6967	amod	capsular,region
R4616	T6982	T6980	nsubj	morphology,is
R4617	T6970	T6967	prep	of,region
R4618	T6971	T6972	amod	younger,lenses
R4619	T6972	T6970	pobj	lenses,of
R462	T1001	T1000	cc	and,alphaA
R4620	T6983	T6982	prep	of,morphology
R4621	T6973	T6957	auxpass	is,known
R4622	T6974	T6957	neg	not,known
R4623	T6975	T6957	prep	at,known
R4624	T6976	T6977	det	this,time
R4625	T6977	T6975	pobj	time,at
R4626	T6978	T6957	punct	", ",known
R4627	T6984	T6985	amod	earlier,stage
R4628	T6985	T6986	compound	stage,lenses
R4629	T6986	T6983	pobj	lenses,of
R463	T1002	T1000	conj	alphaB,alphaA
R4630	T6987	T6980	punct	.,is
R4631	T7085	T7084	prep	from,transformation
R4632	T6989	T6990	amod	Future,studies
R4633	T7086	T7087	det	an,cell
R4634	T7087	T7085	pobj	cell,from
R4635	T7088	T7087	amod	epithelial,cell
R4636	T7089	T7084	prep	into,transformation
R4637	T6990	T6991	nsubjpass	studies,designed
R4638	T7090	T7091	det	a,cell
R4639	T7091	T7089	pobj	cell,into
R464	T1003	T997	prep	within,proportions
R4640	T7092	T7091	compound	fiber,cell
R4641	T6992	T6990	prep	with,studies
R4642	T6993	T6994	amod	defined,objectives
R4643	T7093	T7084	punct	", ",transformation
R4644	T7094	T7095	dep	which,occurs
R4645	T7095	T7084	relcl	occurs,transformation
R4646	T6994	T6992	pobj	objectives,with
R4647	T7096	T7095	prep	at,occurs
R4648	T7097	T7098	det	the,region
R4649	T6995	T6996	aux	to,address
R465	T1004	T1005	det	the,lens
R4650	T7098	T7096	pobj	region,at
R4651	T7099	T7100	amod	equatorial,bow
R4652	T7100	T7098	amod	bow,region
R4653	T6996	T6994	acl	address,objectives
R4654	T7101	T7100	punct	/,bow
R4655	T7102	T7098	prep	of,region
R4656	T6997	T6998	det	the,development
R4657	T7103	T7104	det	the,lens
R4658	T7104	T7102	pobj	lens,of
R4659	T6998	T6996	dobj	development,address
R466	T1005	T1003	pobj	lens,within
R4660	T7105	T7075	punct	.,is
R4661	T7107	T7108	det	The,cell
R4662	T6999	T6998	cc	and,development
R4663	T7108	T7112	nsubj	cell,continues
R4664	T7109	T7110	advmod	newly,forming
R4665	T7110	T7108	amod	forming,cell
R4666	T7000	T6998	conj	progression,development
R4667	T7111	T7108	compound	fiber,cell
R4668	T7001	T6998	prep	of,development
R4669	T7113	T7114	aux	to,differentiate
R467	T1006	T1007	amod	different,functions
R4670	T7114	T7112	xcomp	differentiate,continues
R4671	T7115	T7114	prep	in,differentiate
R4672	T7116	T7117	det	the,cortex
R4673	T7002	T7003	amod	morphological,changes
R4674	T7117	T7115	pobj	cortex,in
R4675	T7118	T7119	mark	until,formed
R4676	T7119	T7114	advcl	formed,differentiate
R4677	T7120	T7121	det	a,cell
R4678	T7121	T7119	nsubjpass	cell,formed
R4679	T7003	T7001	pobj	changes,of
R468	T1007	T994	dobj	functions,suggest
R4680	T7122	T7121	amod	mature,cell
R4681	T7123	T7121	compound	fiber,cell
R4682	T7124	T7121	amod	devoid,cell
R4683	T7125	T7124	prep	of,devoid
R4684	T7004	T7003	acl	seen,changes
R4685	T7126	T7125	pobj	organelles,of
R4686	T7127	T7121	prep	with,cell
R4687	T7128	T7129	compound	suture,formation
R4688	T7005	T7004	prep	in,seen
R4689	T7129	T7127	pobj	formation,with
R469	T1008	T994	prep	for,suggest
R4690	T7130	T7129	prep	at,formation
R4691	T7006	T7007	det	this,study
R4692	T7131	T7132	det	the,ends
R4693	T7132	T7130	pobj	ends,at
R4694	T7133	T7132	prep	of,ends
R4695	T7134	T7135	det	the,cell
R4696	T7135	T7133	pobj	cell,of
R4697	T7007	T7005	pobj	study,in
R4698	T7136	T7119	auxpass	is,formed
R4699	T7137	T7112	punct	.,continues
R47	T184	T182	compound	crystallin,products
R470	T1009	T1010	det	the,subunits
R4700	T7008	T6991	aux	are,designed
R4701	T7139	T7140	det	This,process
R4702	T7140	T7142	nsubjpass	process,defined
R4703	T7141	T7140	amod	entire,process
R4704	T7009	T6991	auxpass	being,designed
R4705	T7143	T7140	prep	from,process
R4706	T7010	T6991	punct	.,designed
R4707	T7144	T7145	amod	epithelial,cell
R4708	T7012	T7013	det	The,differences
R4709	T7145	T7143	pobj	cell,from
R471	T1010	T1008	pobj	subunits,for
R4710	T7146	T7140	prep	to,process
R4711	T7147	T7148	amod	mature,cell
R4712	T7013	T7015	nsubj	differences,were
R4713	T7148	T7146	pobj	cell,to
R4714	T7149	T7148	compound	fiber,cell
R4715	T7150	T7142	auxpass	is,defined
R4716	T7014	T7013	amod	morphological,differences
R4717	T7151	T7142	prep	as,defined
R4718	T7152	T7153	compound	lens,differentiation
R4719	T7153	T7151	pobj	differentiation,as
R472	T1011	T1010	nummod	two,subunits
R4720	T7016	T7013	prep	in,differences
R4721	T7154	T7142	punct	.,defined
R4722	T7156	T7157	det	The,expression
R4723	T7157	T7162	nsubj	expression,be
R4724	T7158	T7157	amod	precise,expression
R4725	T7017	T7018	compound	alphaA,BKO
R4726	T7159	T7157	amod	spatial,expression
R4727	T7160	T7159	cc	and,spatial
R4728	T7161	T7159	conj	temporal,spatial
R4729	T7163	T7157	prep	of,expression
R473	T1012	T1010	prep	in,subunits
R4730	T7164	T7165	det	the,proteins
R4731	T7018	T7020	compound	BKO,lenses
R4732	T7165	T7163	pobj	proteins,of
R4733	T7166	T7165	compound	crystallin,proteins
R4734	T7167	T7157	prep	in,expression
R4735	T7168	T7169	det	the,lens
R4736	T7019	T7018	punct	/,BKO
R4737	T7169	T7167	pobj	lens,in
R4738	T7170	T7169	amod	developing,lens
R4739	T7171	T7162	aux	may,be
R474	T1013	T1014	det	the,lens
R4740	T7020	T7016	pobj	lenses,in
R4741	T7172	T7162	neg	not,be
R4742	T7173	T7162	advmod	simply,be
R4743	T7174	T7175	det	a,consequence
R4744	T7021	T7013	punct	", ",differences
R4745	T7175	T7162	attr	consequence,be
R4746	T7176	T7175	prep	of,consequence
R4747	T7022	T7013	prep	compared,differences
R4748	T7177	T7178	det	the,process
R4749	T7178	T7176	pobj	process,of
R475	T1014	T1012	pobj	lens,in
R4750	T7179	T7178	compound	differentiation,process
R4751	T7023	T7022	prep	to,compared
R4752	T7024	T7025	npadvmod	age,matched
R4753	T7180	T7162	punct	", ",be
R4754	T7181	T7162	cc	but,be
R4755	T7025	T7026	amod	matched,lenses
R4756	T7182	T7183	advmod	instead,play
R4757	T7183	T7162	conj	play,be
R4758	T7184	T7183	aux	may,play
R4759	T7026	T7023	pobj	lenses,to
R476	T1015	T1014	amod	developing,lens
R4760	T7185	T7186	det	an,role
R4761	T7186	T7183	dobj	role,play
R4762	T7027	T7028	amod	wild,type
R4763	T7187	T7186	amod	important,role
R4764	T7188	T7187	punct	", ",important
R4765	T7189	T7187	cc	if,important
R4766	T7028	T7026	compound	type,lenses
R4767	T7190	T7189	neg	not,if
R4768	T7191	T7187	conj	essential,important
R4769	T7029	T7015	punct	", ",were
R477	T1016	T994	punct	.,suggest
R4770	T7030	T7015	acomp	consistent,were
R4771	T7031	T7030	prep	with,consistent
R4772	T7192	T7186	punct	", ",role
R4773	T7032	T7033	det	the,hypothesis
R4774	T7193	T7186	prep	in,role
R4775	T7194	T7195	det	the,process
R4776	T7195	T7193	pobj	process,in
R4777	T7033	T7031	pobj	hypothesis,with
R4778	T7196	T7195	compound	differentiation,process
R4779	T7197	T7195	appos	itself,process
R478	T1018	T1019	advmod	Prior,thought
R4780	T7198	T7162	punct	.,be
R4781	T7200	T7201	det	The,results
R4782	T7201	T7202	nsubj	results,support
R4783	T7034	T7035	mark	that,plays
R4784	T7203	T7201	prep	of,results
R4785	T7204	T7205	det	the,study
R4786	T7035	T7033	acl	plays,hypothesis
R4787	T7205	T7203	pobj	study,of
R4788	T7206	T7205	amod	current,study
R4789	T7036	T7037	compound	alpha,crystallin
R479	T1020	T1018	prep	to,Prior
R4790	T7207	T7208	det	this,hypothesis
R4791	T7208	T7202	dobj	hypothesis,support
R4792	T7209	T7202	punct	.,support
R4793	T7037	T7035	nsubj	crystallin,plays
R4794	T7211	T7212	det	The,mechanisms
R4795	T7212	T7217	nsubj	mechanisms,remain
R4796	T7038	T7037	punct	-,crystallin
R4797	T7213	T7212	amod	exact,mechanisms
R4798	T7214	T7215	advmod	in,vivo
R4799	T7215	T7212	amod	vivo,mechanisms
R48	T185	T184	punct	-,crystallin
R480	T1021	T1022	det	the,1990
R4800	T7039	T7040	det	an,role
R4801	T7216	T7212	amod	molecular,mechanisms
R4802	T7218	T7212	punct	", ",mechanisms
R4803	T7040	T7035	dobj	role,plays
R4804	T7219	T7220	prep	by,influence
R4805	T7220	T7212	relcl	influence,mechanisms
R4806	T7221	T7219	pobj	which,by
R4807	T7041	T7040	amod	active,role
R4808	T7222	T7223	compound	alpha,crystallin
R4809	T7223	T7220	nsubj	crystallin,influence
R481	T1022	T1020	pobj	1990,to
R4810	T7042	T7035	prep	in,plays
R4811	T7224	T7223	punct	-,crystallin
R4812	T7225	T7220	aux	might,influence
R4813	T7226	T7227	nmod	lens,cell
R4814	T7043	T7044	det	the,differentiation
R4815	T7227	T7229	compound	cell,differentiation
R4816	T7228	T7227	amod	epithelial,cell
R4817	T7229	T7220	dobj	differentiation,influence
R4818	T7044	T7042	pobj	differentiation,in
R4819	T7230	T7229	punct	", ",differentiation
R482	T1023	T1022	case	's,1990
R4820	T7231	T7229	cc	and,differentiation
R4821	T7232	T7229	conj	maintenance,differentiation
R4822	T7045	T7044	cc	and,differentiation
R4823	T7233	T7232	prep	of,maintenance
R4824	T7234	T7235	compound	lens,transparency
R4825	T7235	T7233	pobj	transparency,of
R4826	T7046	T7044	conj	growth,differentiation
R4827	T7236	T7217	punct	", ",remain
R4828	T7237	T7238	aux	to,determined
R4829	T7238	T7217	xcomp	determined,remain
R483	T1024	T1019	punct	", ",thought
R4830	T7047	T7044	prep	of,differentiation
R4831	T7239	T7238	auxpass	be,determined
R4832	T7240	T7217	punct	.,remain
R4833	T7048	T7049	compound	lens,fiber
R4834	T7242	T7243	nsubj	AlphaA,are
R4835	T7244	T7242	cc	and,AlphaA
R4836	T7245	T7242	conj	alphaB,AlphaA
R4837	T7246	T7243	attr	members,are
R4838	T7049	T7050	compound	fiber,cells
R4839	T7247	T7246	prep	of,members
R484	T1025	T1026	compound	alpha,crystallin
R4840	T7248	T7249	det	the,family
R4841	T7249	T7247	pobj	family,of
R4842	T7250	T7249	compound	shsp,family
R4843	T7050	T7047	pobj	cells,of
R4844	T7251	T7252	punct	[,7
R4845	T7252	T7243	parataxis	7,are
R4846	T7051	T7015	punct	.,were
R4847	T7253	T7252	punct	],7
R4848	T7254	T7243	punct	.,are
R4849	T7053	T7054	prep	In,was
R485	T1026	T1019	nsubjpass	crystallin,thought
R4850	T7256	T7257	amod	Previous,studies
R4851	T7257	T7258	nsubj	studies,shown
R4852	T7259	T7258	aux	have,shown
R4853	T7055	T7053	pobj	addition,In
R4854	T7260	T7261	mark	that,possesses
R4855	T7261	T7258	ccomp	possesses,shown
R4856	T7262	T7263	det	the,crystallin
R4857	T7263	T7261	nsubj	crystallin,possesses
R4858	T7056	T7054	punct	", ",was
R4859	T7264	T7263	compound	alpha,crystallin
R486	T1027	T1026	punct	-,crystallin
R4860	T7265	T7263	punct	-,crystallin
R4861	T7057	T7054	nsubj	it,was
R4862	T7266	T7267	amod	molecular,activity
R4863	T7267	T7261	dobj	activity,possesses
R4864	T7268	T7267	compound	chaperone,activity
R4865	T7058	T7059	advmod	clearly,evident
R4866	T7059	T7054	acomp	evident,was
R4867	T7269	T7261	punct	", ",possesses
R4868	T7270	T7261	advcl	binding,possesses
R4869	T7060	T7061	mark	that,is
R487	T1028	T1019	auxpass	was,thought
R4870	T7271	T7270	prep	to,binding
R4871	T7272	T7273	advmod	partially,denatured
R4872	T7273	T7274	amod	denatured,proteins
R4873	T7061	T7054	ccomp	is,was
R4874	T7274	T7271	pobj	proteins,to
R4875	T7275	T7270	punct	", ",binding
R4876	T7276	T7277	preconj	both,5
R4877	T7062	T7063	compound	alpha,crystallin
R4878	T7277	T7270	advmod	5,binding
R4879	T7278	T7277	advmod	in,5
R488	T1029	T1030	aux	to,be
R4880	T7063	T7061	nsubj	crystallin,is
R4881	T7279	T7277	advmod	vitro,5
R4882	T7280	T7277	punct	[,5
R4883	T7281	T7277	punct	],5
R4884	T7064	T7063	punct	-,crystallin
R4885	T7282	T7277	cc	and,5
R4886	T7283	T7277	conj	probably,5
R4887	T7284	T7285	advmod	in,6
R4888	T7285	T7283	advmod	6,probably
R4889	T7286	T7285	advmod	vivo,6
R489	T1030	T1019	xcomp	be,thought
R4890	T7065	T7061	acomp	necessary,is
R4891	T7287	T7285	punct	[,6
R4892	T7288	T7285	punct	],6
R4893	T7289	T7270	punct	", ",binding
R4894	T7290	T7291	aux	to,inhibit
R4895	T7066	T7065	prep	for,necessary
R4896	T7291	T7270	advcl	inhibit,binding
R4897	T7292	T7293	amod	further,denaturation
R4898	T7067	T7068	compound	lens,transparency
R4899	T7293	T7291	dobj	denaturation,inhibit
R49	T186	T182	compound	gene,products
R490	T1031	T1032	det	a,protein
R4900	T7294	T7258	punct	.,shown
R4901	T7068	T7066	pobj	transparency,for
R4902	T7296	T7297	mark	Although,be
R4903	T7297	T7301	advcl	be,indicate
R4904	T7069	T7054	punct	.,was
R4905	T7071	T7072	det	The,event
R4906	T7072	T7075	nsubj	event,is
R4907	T7073	T7072	amod	final,event
R4908	T7298	T7299	det	this,property
R4909	T7299	T7297	nsubj	property,be
R491	T1032	T1030	attr	protein,be
R4910	T7300	T7297	aux	may,be
R4911	T7074	T7072	amod	biological,event
R4912	T7302	T7303	det	a,contributor
R4913	T7076	T7072	prep	in,event
R4914	T7303	T7297	attr	contributor,be
R4915	T7304	T7303	amod	major,contributor
R4916	T7305	T7303	prep	to,contributor
R4917	T7306	T7307	det	the,maintenance
R4918	T7077	T7078	det	a,cell
R4919	T7307	T7305	pobj	maintenance,to
R492	T1033	T1032	amod	structural,protein
R4920	T7308	T7307	prep	of,maintenance
R4921	T7309	T7310	compound	lens,clarity
R4922	T7078	T7081	poss	cell,life
R4923	T7310	T7308	pobj	clarity,of
R4924	T7311	T7301	punct	", ",indicate
R4925	T7079	T7078	nmod	lens,cell
R4926	T7312	T7313	det	the,changes
R4927	T7313	T7301	nsubj	changes,indicate
R4928	T7314	T7313	amod	early,changes
R4929	T7080	T7078	amod	epithelial,cell
R493	T1034	T1035	poss	whose,function
R4930	T7315	T7313	prep	in,changes
R4931	T7316	T7317	det	the,lenses
R4932	T7317	T7315	pobj	lenses,in
R4933	T7081	T7076	pobj	life,in
R4934	T7318	T7319	compound	alphaA,BKO
R4935	T7319	T7317	compound	BKO,lenses
R4936	T7082	T7078	case	's,cell
R4937	T7320	T7319	punct	/,BKO
R4938	T7321	T7322	det	a,function
R4939	T7322	T7301	dobj	function,indicate
R494	T1035	T1038	dep	function,was
R4940	T7323	T7324	advmod	much,broader
R4941	T7324	T7322	amod	broader,function
R4942	T7325	T7322	amod	cellular,function
R4943	T7083	T7084	det	the,transformation
R4944	T7326	T7322	prep	for,function
R4945	T7327	T7328	compound	alpha,crystallin
R4946	T7328	T7326	pobj	crystallin,for
R4947	T7329	T7328	punct	-,crystallin
R4948	T7084	T7075	attr	transformation,is
R4949	T7330	T7301	punct	.,indicate
R495	T1036	T1035	amod	major,function
R4950	T7404	T7405	prep	During,increases
R4951	T7332	T7333	compound	Stress,proteins
R4952	T7333	T7334	nsubjpass	proteins,shown
R4953	T7335	T7334	aux	have,shown
R4954	T7406	T7407	amod	myogenic,differentiation
R4955	T7336	T7334	auxpass	been,shown
R4956	T7337	T7338	aux	to,expressed
R4957	T7338	T7334	xcomp	expressed,shown
R4958	T7339	T7338	auxpass	be,expressed
R4959	T7340	T7338	prep	in,expressed
R496	T1037	T1035	amod	cellular,function
R4960	T7341	T7342	amod	non-stressed,cells
R4961	T7407	T7404	pobj	differentiation,During
R4962	T7342	T7340	pobj	cells,in
R4963	T7343	T7338	prep	during,expressed
R4964	T7408	T7405	punct	", ",increases
R4965	T7344	T7343	pobj	development,during
R4966	T7345	T7344	cc	and,development
R4967	T7346	T7344	conj	differentiation,development
R4968	T7409	T7405	nsubj	mRNA,increases
R4969	T7347	T7348	punct	[,25
R497	T1038	T1032	relcl	was,protein
R4970	T7348	T7334	parataxis	25,shown
R4971	T7410	T7409	prep	for,mRNA
R4972	T7349	T7348	punct	],25
R4973	T7350	T7334	punct	.,shown
R4974	T7411	T7410	pobj	alphaB,for
R4975	T7352	T7353	nsubjpass	Hsps,shown
R4976	T7354	T7353	auxpass	were,shown
R4977	T7412	T7405	prep	in,increases
R4978	T7355	T7356	aux	to,expressed
R4979	T7413	T7412	pobj	conjunction,in
R498	T1039	T1040	aux	to,produce
R4980	T7414	T7413	prep	with,conjunction
R4981	T7356	T7353	xcomp	expressed,shown
R4982	T7357	T7356	auxpass	be,expressed
R4983	T7415	T7416	det	the,induction
R4984	T7358	T7356	prep	during,expressed
R4985	T7359	T7360	det	the,differentiation
R4986	T7416	T7414	pobj	induction,with
R4987	T7360	T7358	pobj	differentiation,during
R4988	T7361	T7360	prep	of,differentiation
R4989	T7362	T7363	amod	mammalian,osteoblasts
R499	T1040	T1038	xcomp	produce,was
R4990	T7363	T7361	pobj	osteoblasts,of
R4991	T7364	T7363	cc	and,osteoblasts
R4992	T7365	T7366	amod	promelocytic,cells
R4993	T7417	T7416	prep	of,induction
R4994	T7366	T7363	conj	cells,osteoblasts
R4995	T7367	T7366	compound	leukemia,cells
R4996	T7368	T7369	punct	[,26
R4997	T7418	T7417	pobj	mRNA,of
R4998	T7369	T7353	parataxis	26,shown
R4999	T7370	T7369	punct	],26
R50	T187	T176	aux	have,eliminated
R500	T1041	T1042	det	a,solution
R5000	T7371	T7353	punct	.,shown
R5001	T7419	T7416	prep	for,induction
R5002	T7373	T7374	prep	In,shown
R5003	T7420	T7419	pobj	myogenin,for
R5004	T7375	T7373	pobj	addition,In
R5005	T7376	T7374	punct	", ",shown
R5006	T7421	T7416	punct	", ",induction
R5007	T7377	T7378	compound	hsp,expression
R5008	T7378	T7374	nsubjpass	expression,shown
R5009	T7379	T7374	aux	has,shown
R501	T1042	T1040	dobj	solution,produce
R5010	T7422	T7423	det	the,event
R5011	T7380	T7374	auxpass	been,shown
R5012	T7381	T7382	aux	to,accompany
R5013	T7382	T7374	xcomp	accompany,shown
R5014	T7423	T7416	appos	event,induction
R5015	T7383	T7384	compound	growth,arrest
R5016	T7384	T7382	dobj	arrest,accompany
R5017	T7385	T7384	prep	in,arrest
R5018	T7424	T7423	amod	earliest,event
R5019	T7386	T7387	amod	human,lymphocytes
R502	T1043	T1042	amod	dense,solution
R5020	T7387	T7385	pobj	lymphocytes,in
R5021	T7388	T7387	compound	B,lymphocytes
R5022	T7425	T7423	amod	known,event
R5023	T7389	T7390	punct	[,27
R5024	T7390	T7387	parataxis	27,lymphocytes
R5025	T7391	T7390	punct	],27
R5026	T7426	T7423	prep	in,event
R5027	T7392	T7384	cc	and,arrest
R5028	T7427	T7426	pobj	myogenesis,in
R5029	T7393	T7394	compound	macrophage,differentiation
R503	T1044	T1042	amod	necessary,solution
R5030	T7394	T7384	conj	differentiation,arrest
R5031	T7395	T7394	prep	of,differentiation
R5032	T7396	T7397	nmod	HL,cells
R5033	T7428	T7429	punct	[,29
R5034	T7397	T7395	pobj	cells,of
R5035	T7398	T7397	nummod	60,cells
R5036	T7399	T7400	punct	[,28
R5037	T7429	T7405	parataxis	29,increases
R5038	T7400	T7374	parataxis	28,shown
R5039	T7401	T7400	punct	],28
R504	T1045	T1044	prep	for,necessary
R5040	T7402	T7374	punct	.,shown
R5041	T7430	T7429	punct	],29
R5042	T7431	T7405	punct	.,increases
R5043	T7433	T7434	det	The,addition
R5044	T7434	T7435	nsubjpass	addition,shown
R5045	T7510	T7511	det	the,cycle
R5046	T7511	T7509	dobj	cycle,regulating
R5047	T7436	T7434	prep	of,addition
R5048	T7512	T7511	compound	cell,cycle
R5049	T7513	T7494	punct	.,play
R505	T1046	T1047	det	the,refraction
R5050	T7515	T7516	amod	Several,proteins
R5051	T7437	T7438	amod	exogenous,crystallin
R5052	T7516	T7519	nsubjpass	proteins,found
R5053	T7517	T7518	compound	heat,shock
R5054	T7518	T7516	compound	shock,proteins
R5055	T7438	T7436	pobj	crystallin,of
R5056	T7520	T7519	aux	have,found
R5057	T7521	T7519	auxpass	been,found
R5058	T7439	T7438	compound	alpha,crystallin
R5059	T7522	T7519	prep	in,found
R506	T1047	T1045	pobj	refraction,for
R5060	T7523	T7524	compound	cell,nuclei
R5061	T7440	T7438	punct	-,crystallin
R5062	T7524	T7522	pobj	nuclei,in
R5063	T7525	T7519	prep	in,found
R5064	T7526	T7527	det	the,absence
R5065	T7441	T7434	prep	to,addition
R5066	T7527	T7525	pobj	absence,in
R5067	T7528	T7527	prep	of,absence
R5068	T7529	T7528	pobj	stress,of
R5069	T7442	T7443	amod	primary,epithelium
R507	T1048	T1047	prep	of,refraction
R5070	T7443	T7441	pobj	epithelium,to
R5071	T7530	T7531	punct	[,30
R5072	T7531	T7519	parataxis	30,found
R5073	T7444	T7445	amod	bovine,lens
R5074	T7532	T7531	punct	],30
R5075	T7533	T7519	punct	", ",found
R5076	T7534	T7519	cc	and,found
R5077	T7445	T7443	compound	lens,epithelium
R5078	T7535	T7536	compound	alpha,crystallin
R5079	T7536	T7538	nsubjpass	crystallin,shown
R508	T1049	T1048	pobj	light,of
R5080	T7446	T7435	auxpass	was,shown
R5081	T7537	T7536	punct	-,crystallin
R5082	T7447	T7448	aux	to,induce
R5083	T7448	T7435	xcomp	induce,shown
R5084	T7538	T7519	conj	shown,found
R5085	T7449	T7450	amod	rapid,changes
R5086	T7539	T7538	aux	has,shown
R5087	T7540	T7538	auxpass	been,shown
R5088	T7450	T7448	dobj	changes,induce
R5089	T7541	T7542	aux	to,interact
R509	T1050	T1047	prep	in,refraction
R5090	T7542	T7538	xcomp	interact,shown
R5091	T7451	T7450	prep	in,changes
R5092	T7543	T7542	advmod	directly,interact
R5093	T7544	T7542	prep	with,interact
R5094	T7545	T7544	pobj	DNA,with
R5095	T7546	T7547	punct	[,18
R5096	T7547	T7538	parataxis	18,shown
R5097	T7548	T7547	punct	],18
R5098	T7452	T7453	compound	cell,shape
R5099	T7549	T7538	punct	.,shown
R51	T188	T176	auxpass	been,eliminated
R510	T1051	T1052	det	the,lens
R5100	T7551	T7552	nsubjpass	AlphaB,shown
R5101	T7553	T7551	punct	", ",AlphaB
R5102	T7453	T7451	pobj	shape,in
R5103	T7554	T7551	acl	expressed,AlphaB
R5104	T7555	T7554	prep	in,expressed
R5105	T7556	T7557	amod	transfected,cells
R5106	T7454	T7448	punct	", ",induce
R5107	T7557	T7555	pobj	cells,in
R5108	T7558	T7557	compound	CHO,cells
R5109	T7559	T7552	punct	", ",shown
R511	T1052	T1050	pobj	lens,in
R5110	T7455	T7448	advcl	leading,induce
R5111	T7560	T7552	aux	has,shown
R5112	T7561	T7552	auxpass	been,shown
R5113	T7562	T7563	aux	to,localize
R5114	T7456	T7455	prep	to,leading
R5115	T7563	T7552	xcomp	localize,shown
R5116	T7564	T7563	advmod	ectopically,localize
R5117	T7565	T7563	prep	to,localize
R5118	T7457	T7458	det	the,formation
R5119	T7566	T7567	compound	interphase,nuclei
R512	T1053	T1019	punct	.,thought
R5120	T7567	T7565	pobj	nuclei,to
R5121	T7568	T7552	punct	", ",shown
R5122	T7458	T7456	pobj	formation,to
R5123	T7569	T7552	advcl	suggesting,shown
R5124	T7570	T7571	det	a,role
R5125	T7459	T7458	prep	of,formation
R5126	T7571	T7569	dobj	role,suggesting
R5127	T7572	T7571	amod	regulatory,role
R5128	T7573	T7571	prep	for,role
R5129	T7574	T7575	det	this,protein
R513	T1055	T1056	prep	During,shown
R5130	T7460	T7461	amod	lentoid,bodies
R5131	T7575	T7573	pobj	protein,for
R5132	T7576	T7569	prep	in,suggesting
R5133	T7461	T7459	pobj	bodies,of
R5134	T7577	T7578	det	the,nucleus
R5135	T7578	T7576	pobj	nucleus,in
R5136	T7579	T7580	punct	[,19
R5137	T7462	T7463	punct	[,21
R5138	T7580	T7552	parataxis	19,shown
R5139	T7581	T7580	punct	],19
R514	T1057	T1058	det	the,1990
R5140	T7463	T7435	parataxis	21,shown
R5141	T7582	T7552	punct	.,shown
R5142	T7464	T7463	punct	],21
R5143	T7584	T7585	det	A,subset
R5144	T7585	T7586	nsubjpass	subset,shown
R5145	T7465	T7435	punct	.,shown
R5146	T7587	T7585	prep	of,subset
R5147	T7588	T7589	amod	immortalized,cells
R5148	T7589	T7587	pobj	cells,of
R5149	T7590	T7589	nmod	lens,cells
R515	T1058	T1055	pobj	1990,During
R5150	T7591	T7589	amod	epithelial,cells
R5151	T7592	T7589	prep	from,cells
R5152	T7467	T7468	det	These,studies
R5153	T7593	T7594	compound	alphaBKO,mice
R5154	T7594	T7592	pobj	mice,from
R5155	T7595	T7586	aux	have,shown
R5156	T7596	T7586	auxpass	been,shown
R5157	T7468	T7469	nsubj	studies,suggest
R5158	T7597	T7598	aux	to,hyperproliferate
R5159	T7598	T7586	xcomp	hyperproliferate,shown
R516	T1059	T1058	amod	early,1990
R5160	T7599	T7600	punct	[,20
R5161	T7470	T7469	advmod	strongly,suggest
R5162	T7600	T7598	parataxis	20,hyperproliferate
R5163	T7601	T7600	punct	],20
R5164	T7602	T7598	advcl	suggesting,hyperproliferate
R5165	T7603	T7604	mark	that,be
R5166	T7471	T7472	mark	that,has
R5167	T7604	T7602	ccomp	be,suggesting
R5168	T7605	T7604	nsubj	alphaB,be
R5169	T7472	T7469	ccomp	has,suggest
R517	T1060	T1058	case	's,1990
R5170	T7606	T7604	aux	may,be
R5171	T7607	T7604	acomp	important,be
R5172	T7608	T7604	prep	in,be
R5173	T7473	T7474	det	the,family
R5174	T7609	T7608	pcomp	maintaining,in
R5175	T7610	T7611	amod	genomic,stability
R5176	T7611	T7609	dobj	stability,maintaining
R5177	T7474	T7472	nsubj	family,has
R5178	T7612	T7586	punct	.,shown
R5179	T7614	T7615	prep	In,reported
R518	T1061	T1056	punct	", ",shown
R5180	T7475	T7474	compound	hsp,family
R5181	T7476	T7474	prep	of,family
R5182	T7477	T7476	pobj	proteins,of
R5183	T7478	T7479	amod	other,functions
R5184	T7616	T7617	compound	lens,epithelium
R5185	T7479	T7472	dobj	functions,has
R5186	T7480	T7479	prep	in,functions
R5187	T7617	T7614	pobj	epithelium,In
R5188	T7618	T7617	acl	derived,epithelium
R5189	T7481	T7480	pobj	addition,in
R519	T1062	T1063	compound	alpha,crystallin
R5190	T7619	T7618	prep	from,derived
R5191	T7620	T7621	compound	alphaAKO,lenses
R5192	T7621	T7619	pobj	lenses,from
R5193	T7482	T7481	prep	to,addition
R5194	T7622	T7615	punct	", ",reported
R5195	T7623	T7624	compound	cell,growth
R5196	T7624	T7625	compound	growth,rates
R5197	T7483	T7482	pcomp	protecting,to
R5198	T7625	T7615	nsubjpass	rates,reported
R5199	T7626	T7615	auxpass	were,reported
R52	T189	T158	punct	.,is
R520	T1063	T1056	nsubjpass	crystallin,shown
R5200	T7627	T7628	aux	to,be
R5201	T7628	T7615	xcomp	be,reported
R5202	T7484	T7483	dobj	proteins,protecting
R5203	T7629	T7630	nummod	50,%
R5204	T7630	T7631	npadvmod	%,lower
R5205	T7631	T7628	acomp	lower,be
R5206	T7632	T7628	prep	compared,be
R5207	T7485	T7484	cc	and,proteins
R5208	T7633	T7632	prep	to,compared
R5209	T7634	T7635	amod	wild,type
R521	T1064	T1063	punct	-,crystallin
R5210	T7486	T7484	conj	cells,proteins
R5211	T7635	T7633	pobj	type,to
R5212	T7636	T7637	punct	[,31
R5213	T7637	T7628	parataxis	31,be
R5214	T7487	T7483	prep	during,protecting
R5215	T7638	T7637	punct	],31
R5216	T7639	T7628	punct	", ",be
R5217	T7640	T7628	advcl	suggesting,be
R5218	T7488	T7487	pobj	stress,during
R5219	T7641	T7642	det	a,role
R522	T1065	T1056	auxpass	was,shown
R5220	T7642	T7640	dobj	role,suggesting
R5221	T7643	T7642	prep	for,role
R5222	T7489	T7469	punct	.,suggest
R5223	T7644	T7643	pobj	alphaA,for
R5224	T7645	T7642	prep	in,role
R5225	T7646	T7645	pcomp	regulating,in
R5226	T7491	T7492	compound	Alpha,crystallin
R5227	T7647	T7648	det	the,cycle
R5228	T7648	T7646	dobj	cycle,regulating
R5229	T7649	T7648	compound	cell,cycle
R523	T1066	T1067	aux	to,act
R5230	T7650	T7615	punct	.,reported
R5231	T7492	T7494	nsubj	crystallin,play
R5232	T7652	T7653	nsubj	All,raise
R5233	T7493	T7492	punct	-,crystallin
R5234	T7654	T7652	prep	of,All
R5235	T7655	T7656	det	these,findings
R5236	T7495	T7494	aux	may,play
R5237	T7656	T7654	pobj	findings,of
R5238	T7657	T7658	amod	many,questions
R5239	T7658	T7653	dobj	questions,raise
R524	T1067	T1056	xcomp	act,shown
R5240	T7659	T7658	prep	as,questions
R5241	T7496	T7497	det	a,role
R5242	T7660	T7659	prep	to,as
R5243	T7661	T7662	det	the,s
R5244	T7662	T7660	pobj	s,to
R5245	T7497	T7494	dobj	role,play
R5246	T7663	T7662	amod	possible,s
R5247	T7664	T7662	nmod	role,s
R5248	T7665	T7662	punct	(,s
R5249	T7498	T7497	amod	functional,role
R525	T1068	T1067	prep	as,act
R5250	T7666	T7662	punct	),s
R5251	T7667	T7662	prep	of,s
R5252	T7668	T7669	compound	alpha,crystallin
R5253	T7669	T7667	pobj	crystallin,of
R5254	T7670	T7669	punct	-,crystallin
R5255	T7499	T7494	prep	in,play
R5256	T7671	T7662	prep	in,s
R5257	T7672	T7673	det	the,nucleus
R5258	T7673	T7671	pobj	nucleus,in
R5259	T7500	T7501	det	the,nucleus
R526	T1069	T1070	det	a,chaperone
R5260	T7674	T7671	cc	and,in
R5261	T7675	T7671	conj	in,in
R5262	T7676	T7677	compound	cell,cycle
R5263	T7501	T7499	pobj	nucleus,in
R5264	T7677	T7678	compound	cycle,regulation
R5265	T7678	T7675	pobj	regulation,in
R5266	T7679	T7662	prep	during,s
R5267	T7680	T7679	pobj	differentiation,during
R5268	T7502	T7501	compound	cell,nucleus
R5269	T7681	T7653	punct	.,raise
R527	T1070	T1068	pobj	chaperone,as
R5270	T7503	T7494	cc	and,play
R5271	T7683	T7684	prep	In,is
R5272	T7685	T7686	det	the,study
R5273	T7504	T7505	aux	may,have
R5274	T7686	T7683	pobj	study,In
R5275	T7687	T7686	amod	current,study
R5276	T7505	T7494	conj	have,play
R5277	T7688	T7684	punct	", ",is
R5278	T7689	T7690	det	the,observation
R5279	T7690	T7684	nsubj	observation,is
R528	T1071	T1070	amod	molecular,chaperone
R5280	T7691	T7690	prep	of,observation
R5281	T7506	T7507	det	a,role
R5282	T7692	T7693	det	a,pattern
R5283	T7693	T7691	pobj	pattern,of
R5284	T7507	T7505	dobj	role,have
R5285	T7694	T7693	amod	disorganized,pattern
R5286	T7695	T7693	prep	of,pattern
R5287	T7696	T7695	pobj	nuclei,of
R5288	T7697	T7693	acl	localized,pattern
R5289	T7508	T7505	prep	in,have
R529	T1072	T1067	punct	", ",act
R5290	T7698	T7697	prep	to,localized
R5291	T7699	T7700	det	the,region
R5292	T7509	T7508	pcomp	regulating,in
R5293	T7700	T7698	pobj	region,to
R5294	T7722	T7719	pobj	lenses,of
R5295	T7701	T7702	amod	equatorial,bow
R5296	T7702	T7700	compound	bow,region
R5297	T7723	T7724	compound	alphaA,BKO
R5298	T7703	T7700	prep	of,region
R5299	T7704	T7705	compound	alphaA,BKO
R53	T191	T192	prep	In,compensate
R530	T1073	T1067	advcl	binding,act
R5300	T7705	T7707	compound	BKO,lenses
R5301	T7724	T7722	compound	BKO,lenses
R5302	T7706	T7705	punct	/,BKO
R5303	T7707	T7703	pobj	lenses,of
R5304	T7708	T7693	punct	", ",pattern
R5305	T7709	T7693	cc	and,pattern
R5306	T7710	T7711	amod	nucleic,acid
R5307	T7725	T7724	punct	/,BKO
R5308	T7711	T7712	compound	acid,staining
R5309	T7712	T7693	conj	staining,pattern
R531	T1074	T1073	prep	to,binding
R5310	T7713	T7712	prep	of,staining
R5311	T7726	T7684	punct	", ",is
R5312	T7714	T7713	pobj	structures,of
R5313	T7715	T7712	prep	throughout,staining
R5314	T7727	T7684	acomp	consistent,is
R5315	T7716	T7717	det	the,cortex
R5316	T7717	T7715	pobj	cortex,throughout
R5317	T7718	T7717	amod	anterior,cortex
R5318	T7719	T7717	prep	of,cortex
R5319	T7728	T7727	prep	with,consistent
R532	T1075	T1076	advmod	partially,denatured
R5320	T7720	T7721	nummod	54,wk
R5321	T7721	T7722	compound	wk,lenses
R5322	T7729	T7730	det	a,role
R5323	T7730	T7728	pobj	role,with
R5324	T7731	T7730	prep	for,role
R5325	T7828	T7829	compound	alpha,crystallin
R5326	T7732	T7733	compound	alpha,crystallin
R5327	T7829	T7824	nsubj	crystallin,forms
R5328	T7830	T7829	punct	-,crystallin
R5329	T7831	T7824	advmod	also,forms
R533	T1076	T1077	amod	denatured,proteins
R5330	T7733	T7731	pobj	crystallin,for
R5331	T7832	T7833	det	a,complex
R5332	T7833	T7824	dobj	complex,forms
R5333	T7834	T7824	prep	with,forms
R5334	T7734	T7733	punct	-,crystallin
R5335	T7835	T7836	nmod	type,proteins
R5336	T7836	T7834	pobj	proteins,with
R5337	T7837	T7835	nummod	III,type
R5338	T7735	T7730	prep	in,role
R5339	T7838	T7839	amod	intermediate,filament
R534	T1077	T1074	pobj	proteins,to
R5340	T7839	T7836	compound	filament,proteins
R5341	T7840	T7836	cc	and,proteins
R5342	T7736	T7737	det	the,nucleus
R5343	T7841	T7842	det	the,proteins
R5344	T7842	T7836	conj	proteins,proteins
R5345	T7843	T7844	npadvmod	lens,specific
R5346	T7737	T7735	pobj	nucleus,in
R5347	T7844	T7842	amod	specific,proteins
R5348	T7845	T7844	punct	-,specific
R5349	T7738	T7684	punct	.,is
R535	T1078	T1073	punct	", ",binding
R5350	T7846	T7847	amod	beaded,filament
R5351	T7847	T7842	compound	filament,proteins
R5352	T7848	T7842	appos	CP49,proteins
R5353	T7740	T7741	expl	There,is
R5354	T7849	T7848	cc	and,CP49
R5355	T7850	T7848	conj	CP115,CP49
R5356	T7851	T7824	punct	", ",forms
R5357	T7852	T7853	dep	which,be
R5358	T7853	T7824	advcl	be,forms
R5359	T7854	T7853	aux	may,be
R536	T1079	T1080	preconj	both,5
R5360	T7855	T7853	acomp	critical,be
R5361	T7856	T7855	prep	for,critical
R5362	T7742	T7743	amod	extensive,evidence
R5363	T7857	T7858	amod	proper,assembly
R5364	T7858	T7856	pobj	assembly,for
R5365	T7859	T7858	compound	filament,assembly
R5366	T7860	T7861	punct	[,16
R5367	T7743	T7741	attr	evidence,is
R5368	T7861	T7824	parataxis	16,forms
R5369	T7862	T7861	punct	],16
R537	T1080	T1073	advmod	5,binding
R5370	T7863	T7824	punct	.,forms
R5371	T7744	T7743	prep	from,evidence
R5372	T7865	T7866	amod	Beaded,filament
R5373	T7866	T7867	compound	filament,levels
R5374	T7745	T7746	amod	previous,studies
R5375	T7867	T7869	nsubj	levels,increase
R5376	T7868	T7867	compound	mRNA,levels
R5377	T7746	T7744	pobj	studies,from
R5378	T7870	T7869	advmod	greatly,increase
R5379	T7871	T7869	prep	in,increase
R538	T1081	T1080	advmod	in,5
R5380	T7747	T7743	acl	demonstrating,evidence
R5381	T7872	T7873	amod	differentiating,cells
R5382	T7748	T7749	mark	that,plays
R5383	T7873	T7871	pobj	cells,in
R5384	T7749	T7747	ccomp	plays,demonstrating
R5385	T7874	T7873	nmod	lens,cells
R5386	T7875	T7873	amod	epithelial,cells
R5387	T7876	T7869	punct	", ",increase
R5388	T7750	T7751	compound	alpha,crystallin
R5389	T7877	T7869	cc	and,increase
R539	T1082	T1080	advmod	vitro,5
R5390	T7878	T7879	aux	have,suggested
R5391	T7879	T7869	conj	suggested,increase
R5392	T7751	T7749	nsubj	crystallin,plays
R5393	T7880	T7879	auxpass	been,suggested
R5394	T7881	T7879	prep	as,suggested
R5395	T7752	T7751	punct	-,crystallin
R5396	T7882	T7883	det	a,marker
R5397	T7883	T7881	pobj	marker,as
R5398	T7753	T7754	det	a,role
R5399	T7884	T7883	amod	pan-specific,marker
R54	T193	T194	det	the,mice
R540	T1083	T1080	punct	[,5
R5400	T7885	T7883	prep	for,marker
R5401	T7886	T7887	compound	lens,fiber
R5402	T7754	T7749	dobj	role,plays
R5403	T7887	T7888	compound	fiber,cells
R5404	T7888	T7885	pobj	cells,for
R5405	T7889	T7890	punct	[,17
R5406	T7755	T7749	prep	in,plays
R5407	T7890	T7879	parataxis	17,suggested
R5408	T7891	T7890	punct	],17
R5409	T7892	T7869	punct	.,increase
R541	T1084	T1080	punct	],5
R5410	T7894	T7895	nsubj	It,is
R5411	T7756	T7757	det	the,organization
R5412	T7896	T7895	advmod	therefore,is
R5413	T7897	T7895	acomp	possible,is
R5414	T7757	T7755	pobj	organization,in
R5415	T7898	T7899	mark	that,have
R5416	T7899	T7895	ccomp	have,is
R5417	T7900	T7901	amod	increased,synthesis
R5418	T7901	T7899	nsubj	synthesis,have
R5419	T7758	T7757	amod	cytoskeletal,organization
R542	T1085	T1080	cc	and,5
R5420	T7902	T7901	prep	of,synthesis
R5421	T7903	T7904	compound	alpha,crystallin
R5422	T7904	T7902	pobj	crystallin,of
R5423	T7759	T7741	punct	.,is
R5424	T7905	T7904	punct	-,crystallin
R5425	T7906	T7901	prep	in,synthesis
R5426	T7907	T7908	amod	epithelial,cells
R5427	T7761	T7762	preconj	Both,alphaA
R5428	T7908	T7906	pobj	cells,in
R5429	T7909	T7901	advmod	early,synthesis
R543	T1086	T1080	conj	probably,5
R5430	T7910	T7909	prep	in,early
R5431	T7762	T7763	nsubj	alphaA,bind
R5432	T7911	T7912	det	the,process
R5433	T7912	T7910	pobj	process,in
R5434	T7913	T7912	compound	differentiation,process
R5435	T7914	T7899	aux	may,have
R5436	T7764	T7762	cc	and,alphaA
R5437	T7915	T7916	amod	profound,effects
R5438	T7916	T7899	dobj	effects,have
R5439	T7917	T7899	prep	upon,have
R544	T1087	T1086	advmod	in,probably
R5440	T7765	T7762	conj	alphaB,alphaA
R5441	T7918	T7919	det	the,cytoskeleton
R5442	T7919	T7917	pobj	cytoskeleton,upon
R5443	T7920	T7919	punct	", ",cytoskeleton
R5444	T7766	T7763	aux	can,bind
R5445	T7921	T7922	dep	which,affect
R5446	T7922	T7919	relcl	affect,cytoskeleton
R5447	T7923	T7922	prep	in,affect
R5448	T7767	T7763	advmod	specifically,bind
R5449	T7924	T7923	pobj	turn,in
R545	T1088	T1086	advmod	vivo,probably
R5450	T7925	T7922	aux	may,affect
R5451	T7768	T7763	prep	to,bind
R5452	T7769	T7768	pobj	actin,to
R5453	T7926	T7922	advmod	profoundly,affect
R5454	T7770	T7763	punct	", ",bind
R5455	T7927	T7928	compound	cell,shape
R5456	T7928	T7922	dobj	shape,affect
R5457	T7771	T7772	preconj	both,13
R5458	T7929	T7928	cc	and,shape
R5459	T7930	T7928	conj	migration,shape
R546	T1089	T1090	punct	[,6
R5460	T7772	T7763	advmod	13,bind
R5461	T7931	T7895	punct	.,is
R5462	T7933	T7934	det	The,lack
R5463	T7773	T7772	advmod	in,13
R5464	T7774	T7772	advmod	vitro,13
R5465	T7934	T7935	nsubj	lack,supports
R5466	T7775	T7772	punct	[,13
R5467	T7936	T7934	prep	of,lack
R5468	T7776	T7772	punct	],13
R5469	T7937	T7938	amod	cellar,organization
R547	T1090	T1086	parataxis	6,probably
R5470	T7938	T7936	pobj	organization,of
R5471	T7939	T7938	cc	and,organization
R5472	T7777	T7772	cc	and,13
R5473	T7940	T7941	amod	uniform,shape
R5474	T7941	T7938	conj	shape,organization
R5475	T7778	T7779	advmod	in,14
R5476	T7942	T7941	compound	cell,shape
R5477	T7943	T7934	prep	at,lack
R5478	T7944	T7945	det	the,region
R5479	T7779	T7772	conj	14,13
R548	T1091	T1090	punct	],6
R5480	T7945	T7943	pobj	region,at
R5481	T7946	T7945	amod	equatorial,region
R5482	T7947	T7934	acl	observed,lack
R5483	T7780	T7779	advmod	vivo,14
R5484	T7948	T7947	prep	in,observed
R5485	T7949	T7950	compound	alphaA,BKO
R5486	T7781	T7779	punct	[,14
R5487	T7950	T7952	compound	BKO,lenses
R5488	T7951	T7950	punct	/,BKO
R5489	T7952	T7948	pobj	lenses,in
R549	T1092	T1073	punct	", ",binding
R5490	T7782	T7779	punct	],14
R5491	T7953	T7954	det	this,hypothesis
R5492	T7954	T7935	dobj	hypothesis,supports
R5493	T7955	T7935	punct	.,supports
R5494	T7783	T7763	punct	.,bind
R5495	T7957	T7958	nsubj	Studies,are
R5496	T7785	T7786	compound	Actin,filament
R5497	T7959	T7960	advmod	currently,underway
R5498	T7960	T7958	acomp	underway,are
R5499	T7786	T7787	compound	filament,formation
R55	T194	T191	pobj	mice,In
R550	T1093	T1094	aux	to,inhibit
R5500	T7787	T7788	nsubjpass	formation,shown
R5501	T7788	T7791	ccomp	shown,known
R5502	T7961	T7962	aux	to,characterize
R5503	T7789	T7788	aux	has,shown
R5504	T7962	T7958	advcl	characterize,are
R5505	T7963	T7964	amod	cytoskeletal,organization
R5506	T7964	T7962	dobj	organization,characterize
R5507	T7790	T7788	auxpass	been,shown
R5508	T7965	T7962	prep	in,characterize
R5509	T7966	T7967	det	the,lens
R551	T1094	T1073	advcl	inhibit,binding
R5510	T7792	T7793	aux	to,be
R5511	T7967	T7965	pobj	lens,in
R5512	T7968	T7969	compound	alphaA,BKO
R5513	T7969	T7967	compound	BKO,lens
R5514	T7970	T7969	punct	/,BKO
R5515	T7971	T7958	punct	.,are
R5516	T7793	T7788	xcomp	be,shown
R5517	T7794	T7793	acomp	necessary,be
R5518	T7795	T7794	prep	for,necessary
R5519	T7796	T7797	det	the,differentiation
R552	T1095	T1096	amod	further,denaturation
R5520	T7797	T7795	pobj	differentiation,for
R5521	T7798	T7797	prep	of,differentiation
R5522	T7799	T7800	nmod	lens,cells
R5523	T7800	T7798	pobj	cells,of
R5524	T7801	T7800	amod	epithelial,cells
R5525	T7802	T7803	punct	[,15
R5526	T7803	T7788	parataxis	15,shown
R5527	T7804	T7803	punct	],15
R5528	T7805	T7791	punct	", ",known
R5529	T7806	T7791	advmod	however,known
R553	T1096	T1094	dobj	denaturation,inhibit
R5530	T7807	T7791	punct	", ",known
R5531	T7808	T7809	det	the,significance
R5532	T7809	T7791	nsubjpass	significance,known
R5533	T7810	T7809	prep	of,significance
R5534	T7811	T7812	compound	alpha,crystallin
R5535	T7812	T7814	compound	crystallin,interaction
R5536	T7813	T7812	punct	-,crystallin
R5537	T7814	T7810	pobj	interaction,of
R5538	T7815	T7814	prep	with,interaction
R5539	T7816	T7815	pobj	actin,with
R554	T1097	T1096	cc	and,denaturation
R5540	T7817	T7814	prep	in,interaction
R5541	T7818	T7817	pobj	differentiation,in
R5542	T7819	T7791	auxpass	is,known
R5543	T7820	T7791	neg	not,known
R5544	T7821	T7791	punct	.,known
R5545	T7823	T7824	prep	In,forms
R5546	T7825	T7826	det	the,lens
R5547	T7826	T7823	pobj	lens,In
R5548	T7827	T7824	punct	", ",forms
R5549	T7972	T7973	det	The,history
R555	T1098	T1096	conj	aggregation,denaturation
R5550	T7973	T7975	nsubjpass	history,accessed
R5551	T7974	T7973	amod	pre-publication,history
R5552	T7976	T7973	prep	for,history
R5553	T7977	T7978	det	this,paper
R5554	T7978	T7976	pobj	paper,for
R5555	T7979	T7975	aux	can,accessed
R5556	T7980	T7975	auxpass	be,accessed
R5557	T7981	T7975	advmod	here,accessed
R5558	T7982	T7975	punct	:,accessed
R556	T1099	T1096	prep	of,denaturation
R5561	T8033	T8034	compound	Light,micrographs
R5562	T8035	T8034	prep	of,micrographs
R5563	T8036	T8035	pobj	lenses,of
R5564	T8037	T8036	acl	dissected,lenses
R5565	T8038	T8037	prep	from,dissected
R5566	T8039	T8040	amod	fixed,eyes
R5567	T8040	T8038	pobj	eyes,from
R5568	T8041	T8040	prep	of,eyes
R5569	T8042	T8043	compound	alphaA,BKO
R557	T1100	T1101	compound	lens,proteins
R5570	T8043	T8041	pobj	BKO,of
R5571	T8044	T8043	punct	/,BKO
R5572	T8045	T8046	punct	(,A
R5573	T8046	T8034	parataxis	A,micrographs
R5574	T8047	T8048	punct	-,C
R5575	T8048	T8046	prep	C,A
R5576	T8049	T8046	punct	),A
R5577	T8050	T8034	cc	and,micrographs
R5578	T8051	T8052	amod	wild,type
R5579	T8052	T8053	nmod	type,mice
R558	T1101	T1099	pobj	proteins,of
R5580	T8053	T8034	conj	mice,micrographs
R5581	T8054	T8055	punct	(,D
R5582	T8055	T8053	parataxis	D,mice
R5583	T8056	T8057	punct	-,F
R5584	T8057	T8055	prep	F,D
R5585	T8058	T8055	punct	),D
R5586	T8059	T8053	punct	.,mice
R5587	T8061	T8062	det	All,micrographs
R5588	T8062	T8063	nsubjpass	micrographs,generated
R5589	T8064	T8063	auxpass	were,generated
R559	T1102	T1056	punct	.,shown
R5590	T8065	T8063	advcl	using,generated
R5591	T8066	T8067	det	a,objective
R5592	T8067	T8065	dobj	objective,using
R5593	T8068	T8067	nummod	4,objective
R5594	T8069	T8070	punct	×,0.10
R5595	T8070	T8068	prep	0.10,4
R5596	T8071	T8070	punct	/,0.10
R5597	T8072	T8067	prep	on,objective
R5598	T8073	T8074	det	a,LSM
R5599	T8074	T8072	pobj	LSM,on
R56	T195	T196	amod	single,knockout
R560	T1104	T1105	nsubjpass	AlphaA,shown
R5600	T8075	T8074	compound	Zeiss,LSM
R5601	T8076	T8074	nummod	410,LSM
R5602	T8077	T8074	acl	using,LSM
R5603	T8078	T8079	amod	transmitted,light
R5604	T8079	T8080	compound	light,mode
R5605	T8080	T8077	dobj	mode,using
R5606	T8081	T8063	punct	.,generated
R5607	T8083	T8084	nsubj	Images,were
R5608	T8085	T8083	prep	in,Images
R5609	T8086	T8085	punct	(,in
R561	T1106	T1104	cc	and,AlphaA
R5610	T8087	T8088	nmod	A,B
R5611	T8088	T8085	pobj	B,in
R5612	T8089	T8088	punct	&,B
R5613	T8090	T8084	punct	),were
R5614	T8091	T8084	acomp	enlarged,were
R5615	T8092	T8084	prep	compared,were
R5616	T8093	T8092	prep	to,compared
R5617	T8094	T8095	nmod	images,C
R5618	T8095	T8093	pobj	C,to
R5619	T8096	T8095	punct	(,C
R562	T1107	T1104	conj	alphaB,AlphaA
R5620	T8097	T8098	punct	-,F
R5621	T8098	T8095	prep	F,C
R5622	T8099	T8095	punct	),C
R5623	T8100	T8101	punct	(,see
R5624	T8101	T8095	parataxis	see,C
R5625	T8102	T8103	compound	scale,bars
R5626	T8103	T8101	dobj	bars,see
R5627	T8104	T8101	punct	),see
R5628	T8105	T8084	punct	.,were
R5629	T8107	T8108	prep	For,set
R563	T1108	T1105	auxpass	were,shown
R5630	T8109	T8110	det	all,micrographs
R5631	T8110	T8107	pobj	micrographs,For
R5632	T8111	T8110	prep	except,micrographs
R5633	T8112	T8111	punct	(,except
R5634	T8113	T8111	pobj	B,except
R5635	T8114	T8108	punct	),set
R5636	T8115	T8116	det	the,point
R5637	T8116	T8108	nsubjpass	point,set
R5638	T8117	T8116	amod	objective,point
R5639	T8118	T8116	amod	focal,point
R564	T1109	T1105	advmod	also,shown
R5640	T8119	T8108	auxpass	was,set
R5641	T8120	T8108	prep	at,set
R5642	T8121	T8122	det	the,plane
R5643	T8122	T8120	pobj	plane,at
R5644	T8123	T8122	amod	equatorial,plane
R5645	T8124	T8122	prep	of,plane
R5646	T8125	T8126	det	the,lens
R5647	T8126	T8124	pobj	lens,of
R5648	T8127	T8108	punct	", ",set
R5649	T8128	T8129	mark	while,set
R565	T1110	T1111	aux	to,have
R5650	T8129	T8108	advcl	set,set
R5651	T8130	T8129	punct	(,set
R5652	T8131	T8129	nsubjpass	B,set
R5653	T8132	T8129	punct	),set
R5654	T8133	T8129	auxpass	was,set
R5655	T8134	T8129	prep	at,set
R5656	T8135	T8136	det	the,pole
R5657	T8136	T8134	pobj	pole,at
R5658	T8137	T8136	amod	posterior,pole
R5659	T8138	T8136	prep	of,pole
R566	T1111	T1105	xcomp	have,shown
R5660	T8139	T8140	det	the,lens
R5661	T8140	T8138	pobj	lens,of
R5662	T8141	T8108	punct	.,set
R5663	T8143	T8144	punct	(,A
R5664	T8144	T8145	meta	A,micrographs
R5665	T8146	T8144	punct	&,A
R5666	T8147	T8144	dep	B,A
R5667	T8148	T8144	punct	),A
R5668	T8149	T8145	amod	Representative,micrographs
R5669	T8150	T8145	prep	of,micrographs
R567	T1112	T1113	compound	sequence,homology
R5670	T8151	T8150	pobj	lenses,of
R5671	T8152	T8151	prep	from,lenses
R5672	T8153	T8154	nummod	5,wk
R5673	T8154	T8155	compound	wk,mice
R5674	T8155	T8152	pobj	mice,from
R5675	T8156	T8157	compound	alphaA,BKO
R5676	T8157	T8155	compound	BKO,mice
R5677	T8158	T8157	punct	/,BKO
R5678	T8159	T8145	punct	", ",micrographs
R5679	T8160	T8145	acl	showing,micrographs
R568	T1113	T1111	dobj	homology,have
R5680	T8161	T8160	dobj	changes,showing
R5681	T8162	T8160	prep	in,showing
R5682	T8163	T8164	det	the,regions
R5683	T8164	T8162	pobj	regions,in
R5684	T8165	T8164	amod	equatorial,regions
R5685	T8166	T8167	punct	(,A
R5686	T8167	T8165	parataxis	A,equatorial
R5687	T8168	T8167	punct	),A
R5688	T8169	T8165	cc	and,equatorial
R5689	T8170	T8165	conj	posterior,equatorial
R569	T1114	T1113	prep	with,homology
R5690	T8171	T8172	advmod	sub,capsular
R5691	T8172	T8164	amod	capsular,regions
R5692	T8173	T8174	punct	(,B
R5693	T8174	T8164	parataxis	B,regions
R5694	T8175	T8174	punct	),B
R5695	T8176	T8145	punct	.,micrographs
R5696	T8178	T8179	punct	(,C
R5697	T8179	T8180	meta	C,micrograph
R5698	T8181	T8179	punct	),C
R5699	T8182	T8180	amod	Representative,micrograph
R57	T196	T194	compound	knockout,mice
R570	T1115	T1116	amod	several,proteins
R5700	T8183	T8180	prep	of,micrograph
R5701	T8184	T8185	nummod	54,wk
R5702	T8185	T8186	compound	wk,lens
R5703	T8186	T8183	pobj	lens,of
R5704	T8187	T8188	compound	alphaA,BKO
R5705	T8188	T8186	compound	BKO,lens
R5706	T8189	T8188	punct	/,BKO
R5707	T8190	T8186	compound	mouse,lens
R5708	T8191	T8186	acl	showing,lens
R5709	T8192	T8193	amod	dense,cataract
R571	T1116	T1114	pobj	proteins,with
R5710	T8193	T8191	dobj	cataract,showing
R5711	T8194	T8195	amod	whole,lens
R5712	T8195	T8193	compound	lens,cataract
R5713	T8196	T8180	punct	.,micrograph
R5714	T8198	T8199	punct	(,D
R5715	T8199	T8200	meta	D,lenses
R5716	T8201	T8199	punct	-,D
R5717	T8202	T8199	dep	F,D
R5718	T8203	T8199	punct	),D
R5719	T8204	T8200	amod	Representative,lenses
R572	T1117	T1116	amod	other,proteins
R5720	T8205	T8200	amod	clear,lenses
R5721	T8206	T8200	prep	from,lenses
R5722	T8207	T8208	nummod	5,wk
R5723	T8208	T8209	nmod	wk,mice
R5724	T8209	T8206	pobj	mice,from
R5725	T8210	T8211	punct	(,D
R5726	T8211	T8208	parataxis	D,wk
R5727	T8212	T8211	punct	),D
R5728	T8213	T8208	punct	", ",wk
R5729	T8214	T8215	nummod	46,wk
R573	T1118	T1119	dep	that,are
R5730	T8215	T8208	conj	wk,wk
R5731	T8216	T8217	punct	(,E
R5732	T8217	T8215	parataxis	E,wk
R5733	T8218	T8217	punct	),E
R5734	T8219	T8215	punct	", ",wk
R5735	T8220	T8215	cc	and,wk
R5736	T8221	T8215	conj	wk,wk
R5737	T8222	T8221	nummod	72,wk
R5738	T8223	T8224	punct	(,F
R5739	T8224	T8221	parataxis	F,wk
R574	T1119	T1116	relcl	are,proteins
R5740	T8225	T8224	punct	),F
R5741	T8226	T8227	amod	wild,type
R5742	T8227	T8209	compound	type,mice
R5743	T8228	T8200	punct	.,lenses
R5744	T8230	T8231	nsubj	Arrows,indicate
R5745	T8232	T8233	punct	(,A
R5746	T8233	T8230	parataxis	A,Arrows
R5747	T8234	T8233	punct	),A
R5748	T8235	T8231	dobj	vacuoles,indicate
R5749	T8236	T8235	prep	in,vacuoles
R575	T1120	T1119	attr	members,are
R5750	T8237	T8238	det	the,region
R5751	T8238	T8236	pobj	region,in
R5752	T8239	T8238	amod	equatorial,region
R5753	T8240	T8238	prep	of,region
R5754	T8241	T8242	det	a,lens
R5755	T8242	T8240	pobj	lens,of
R5756	T8243	T8242	amod	representative,lens
R5757	T8244	T8245	nummod	5,wk
R5758	T8245	T8242	compound	wk,lens
R5759	T8246	T8247	compound	alphaA,BKO
R576	T1121	T1120	prep	of,members
R5760	T8247	T8242	compound	BKO,lens
R5761	T8248	T8247	punct	/,BKO
R5762	T8249	T8231	punct	.,indicate
R5763	T8251	T8252	det	The,arrowheads
R5764	T8252	T8253	nsubj	arrowheads,indicate
R5765	T8254	T8255	punct	(,B
R5766	T8255	T8252	parataxis	B,arrowheads
R5767	T8256	T8255	punct	),B
R5768	T8257	T8258	det	an,area
R5769	T8258	T8253	dobj	area,indicate
R577	T1122	T1123	det	the,family
R5770	T8259	T8258	prep	of,area
R5771	T8260	T8261	amod	minor,light
R5772	T8261	T8259	pobj	light,of
R5773	T8262	T8261	acl	scattering,light
R5774	T8263	T8262	advmod	deep,scattering
R5775	T8264	T8262	prep	to,scattering
R5776	T8265	T8266	det	the,capsule
R5777	T8266	T8264	pobj	capsule,to
R5778	T8267	T8266	amod	posterior,capsule
R5779	T8268	T8258	prep	in,area
R578	T1123	T1121	pobj	family,of
R5780	T8269	T8270	det	a,lens
R5781	T8270	T8268	pobj	lens,in
R5782	T8271	T8270	amod	representative,lens
R5783	T8272	T8273	nummod	5,wk
R5784	T8273	T8270	compound	wk,lens
R5785	T8274	T8275	compound	alphaA,BKO
R5786	T8275	T8270	compound	BKO,lens
R5787	T8276	T8275	punct	/,BKO
R5788	T8277	T8253	punct	.,indicate
R5789	T8300	T8301	amod	Representative,sections
R579	T1124	T1123	amod	small,family
R5790	T8302	T8301	amod	confocal,sections
R5791	T8303	T8301	amod	optical,sections
R5792	T8304	T8301	acl	taken,sections
R5793	T8305	T8304	prep	from,taken
R5794	T8306	T8307	amod	mid-axial,sections
R5795	T8307	T8305	pobj	sections,from
R5796	T8308	T8307	compound	vibratome,sections
R5797	T8309	T8307	prep	of,sections
R5798	T8310	T8311	compound	alphaA,BKO
R5799	T8311	T8309	pobj	BKO,of
R58	T197	T198	compound	alpha,crystallin
R580	T1125	T1126	nmod	heat,shock
R5800	T8312	T8311	punct	/,BKO
R5801	T8313	T8314	punct	(,B
R5802	T8314	T8307	parataxis	B,sections
R5803	T8315	T8314	nmod	A,B
R5804	T8316	T8314	punct	&,B
R5805	T8317	T8314	punct	),B
R5806	T8318	T8307	cc	and,sections
R5807	T8319	T8320	amod	wild,type
R5808	T8320	T8321	nmod	type,lenses
R5809	T8321	T8307	conj	lenses,sections
R581	T1126	T1127	nmod	shock,protein
R5810	T8322	T8323	punct	(,C
R5811	T8323	T8321	parataxis	C,lenses
R5812	T8324	T8325	punct	-,E
R5813	T8325	T8323	prep	E,C
R5814	T8326	T8323	punct	),C
R5815	T8327	T8301	punct	.,sections
R5816	T8329	T8330	det	All,sections
R5817	T8330	T8332	nsubjpass	sections,generated
R5818	T8331	T8330	amod	optical,sections
R5819	T8333	T8332	auxpass	were,generated
R582	T1127	T1123	nmod	protein,family
R5820	T8334	T8332	advcl	using,generated
R5821	T8335	T8336	det	a,objective
R5822	T8336	T8334	dobj	objective,using
R5823	T8337	T8336	nummod	4,objective
R5824	T8338	T8339	punct	×,0.10
R5825	T8339	T8337	prep	0.10,4
R5826	T8340	T8339	punct	/,0.10
R5827	T8341	T8336	prep	on,objective
R5828	T8342	T8343	det	a,LSM
R5829	T8343	T8341	pobj	LSM,on
R583	T1128	T1127	punct	(,protein
R5830	T8344	T8343	compound	Zeiss,LSM
R5831	T8345	T8343	nummod	410,LSM
R5832	T8346	T8332	punct	.,generated
R5833	T8348	T8349	det	The,color
R5834	T8349	T8351	nsubj	color,represents
R5835	T8350	T8349	amod	red,color
R5836	T8352	T8353	compound	DiI,staining
R5837	T8353	T8351	dobj	staining,represents
R5838	T8354	T8355	punct	(,membrane
R5839	T8355	T8353	parataxis	membrane,staining
R584	T1129	T1127	appos	hsp,protein
R5840	T8356	T8355	punct	),membrane
R5841	T8357	T8351	punct	", ",represents
R5842	T8358	T8359	mark	while,represents
R5843	T8359	T8351	advcl	represents,represents
R5844	T8360	T8361	det	the,color
R5845	T8361	T8359	nsubj	color,represents
R5846	T8362	T8361	amod	green,color
R5847	T8363	T8364	npadvmod	SYTOX,green
R5848	T8364	T8365	amod	green,staining
R5849	T8365	T8359	dobj	staining,represents
R585	T1130	T1123	punct	),family
R5850	T8366	T8367	punct	(,DNA
R5851	T8367	T8359	parataxis	DNA,represents
R5852	T8368	T8367	punct	),DNA
R5853	T8369	T8351	punct	.,represents
R5854	T8371	T8372	punct	(,taken
R5855	T8373	T8372	nsubjpass	A,taken
R5856	T8374	T8373	cc	&,A
R5857	T8375	T8373	conj	B,A
R5858	T8376	T8372	punct	),taken
R5859	T8377	T8372	auxpass	were,taken
R586	T1131	T1132	punct	[,7
R5860	T8378	T8372	prep	from,taken
R5861	T8379	T8380	nummod	5,wk
R5862	T8380	T8381	nmod	wk,lenses
R5863	T8381	T8378	pobj	lenses,from
R5864	T8382	T8380	cc	and,wk
R5865	T8383	T8384	nummod	54,wk
R5866	T8384	T8380	conj	wk,wk
R5867	T8385	T8386	compound	alphaA,BKO
R5868	T8386	T8381	compound	BKO,lenses
R5869	T8387	T8386	punct	/,BKO
R587	T1132	T1105	parataxis	7,shown
R5870	T8388	T8372	punct	", ",taken
R5871	T8389	T8372	advmod	respectively,taken
R5872	T8390	T8372	punct	.,taken
R5873	T8392	T8393	punct	(,taken
R5874	T8394	T8393	nsubjpass	C,taken
R5875	T8395	T8396	punct	-,E
R5876	T8396	T8394	prep	E,C
R5877	T8397	T8393	punct	),taken
R5878	T8398	T8393	auxpass	were,taken
R5879	T8399	T8393	prep	from,taken
R588	T1133	T1132	punct	],7
R5880	T8400	T8401	nummod	5,wk
R5881	T8401	T8402	nmod	wk,lenses
R5882	T8402	T8399	pobj	lenses,from
R5883	T8403	T8401	punct	", ",wk
R5884	T8404	T8405	nummod	46,wk
R5885	T8405	T8401	conj	wk,wk
R5886	T8406	T8405	punct	", ",wk
R5887	T8407	T8405	cc	and,wk
R5888	T8408	T8409	nummod	72,wk
R5889	T8409	T8405	conj	wk,wk
R589	T1134	T1105	punct	.,shown
R5890	T8410	T8411	amod	wild,type
R5891	T8411	T8402	compound	type,lenses
R5892	T8412	T8393	punct	", ",taken
R5893	T8413	T8393	advmod	respectively,taken
R5894	T8414	T8393	punct	.,taken
R5895	T8416	T8417	det	The,arrowheads
R5896	T8417	T8418	nsubj	arrowheads,indicate
R5897	T8419	T8420	amod	representative,nuclei
R5898	T8420	T8418	dobj	nuclei,indicate
R5899	T8421	T8420	amod	anterior,nuclei
R59	T198	T196	compound	crystallin,knockout
R590	T1136	T1137	advmod	Moreover,shown
R5900	T8422	T8420	amod	epithelial,nuclei
R5901	T8423	T8420	acl	stained,nuclei
R5902	T8424	T8423	prep	with,stained
R5903	T8425	T8426	compound	SYTOX,green
R5904	T8426	T8424	pobj	green,with
R5905	T8427	T8418	punct	.,indicate
R5906	T8471	T8472	amod	Representative,sections
R5907	T8473	T8472	amod	confocal,sections
R5908	T8474	T8472	amod	optical,sections
R5909	T8475	T8472	prep	from,sections
R591	T1138	T1137	punct	", ",shown
R5910	T8476	T8477	nmod	alphaA,BKO
R5911	T8477	T8479	nmod	BKO,lenses
R5912	T8478	T8477	punct	/,BKO
R5913	T8479	T8475	pobj	lenses,from
R5914	T8480	T8481	punct	(,A
R5915	T8481	T8477	parataxis	A,BKO
R5916	T8482	T8483	punct	-,J
R5917	T8483	T8481	prep	J,A
R5918	T8484	T8481	punct	),A
R5919	T8485	T8477	cc	and,BKO
R592	T1139	T1137	nsubjpass	expression,shown
R5920	T8486	T8487	amod	wild,type
R5921	T8487	T8477	conj	type,BKO
R5922	T8488	T8489	punct	(,K
R5923	T8489	T8487	parataxis	K,type
R5924	T8490	T8491	punct	-,T
R5925	T8491	T8489	prep	T,K
R5926	T8492	T8489	punct	),K
R5927	T8493	T8472	punct	.,sections
R5928	T8495	T8496	det	All,sections
R5929	T8496	T8498	nsubjpass	sections,generated
R593	T1140	T1139	prep	of,expression
R5930	T8497	T8496	amod	optical,sections
R5931	T8499	T8498	auxpass	were,generated
R5932	T8500	T8498	advcl	using,generated
R5933	T8501	T8502	det	a,objective
R5934	T8502	T8500	dobj	objective,using
R5935	T8503	T8502	nummod	63,objective
R5936	T8504	T8505	punct	×,1.4
R5937	T8505	T8503	prep	1.4,63
R5938	T8506	T8505	punct	/,1.4
R5939	T8507	T8502	prep	on,objective
R594	T1141	T1142	compound	alpha,crystallin
R5940	T8508	T8509	det	a,LSM
R5941	T8509	T8507	pobj	LSM,on
R5942	T8510	T8509	compound	Zeiss,LSM
R5943	T8511	T8509	nummod	410,LSM
R5944	T8512	T8498	punct	.,generated
R5945	T8514	T8515	det	The,color
R5946	T8515	T8517	nsubj	color,represents
R5947	T8516	T8515	amod	red,color
R5948	T8518	T8519	compound	DiI,staining
R5949	T8519	T8517	dobj	staining,represents
R595	T1142	T1140	pobj	crystallin,of
R5950	T8520	T8521	punct	(,membrane
R5951	T8521	T8519	parataxis	membrane,staining
R5952	T8522	T8521	punct	),membrane
R5953	T8523	T8517	punct	", ",represents
R5954	T8524	T8525	mark	while,represents
R5955	T8525	T8517	advcl	represents,represents
R5956	T8526	T8527	det	the,color
R5957	T8527	T8525	nsubj	color,represents
R5958	T8528	T8527	amod	green,color
R5959	T8529	T8530	npadvmod	SYTOX,green
R596	T1143	T1142	punct	-,crystallin
R5960	T8530	T8531	amod	green,staining
R5961	T8531	T8525	dobj	staining,represents
R5962	T8532	T8533	punct	(,DNA
R5963	T8533	T8531	parataxis	DNA,staining
R5964	T8534	T8533	punct	),DNA
R5965	T8535	T8517	punct	.,represents
R5966	T8537	T8538	punct	(,are
R5967	T8539	T8538	nsubj	A,are
R5968	T8540	T8541	punct	-,E
R5969	T8541	T8539	prep	E,A
R597	T1144	T1142	punct	", ",crystallin
R5970	T8542	T8538	punct	),are
R5971	T8543	T8538	prep	from,are
R5972	T8544	T8545	nummod	5,wk
R5973	T8545	T8546	compound	wk,lenses
R5974	T8546	T8543	pobj	lenses,from
R5975	T8547	T8548	compound	alphaA,BKO
R5976	T8548	T8546	compound	BKO,lenses
R5977	T8549	T8548	punct	/,BKO
R5978	T8550	T8538	punct	.,are
R5979	T8552	T8553	punct	(,are
R598	T1145	T1146	advmod	particularly,alphaB
R5980	T8554	T8553	nsubj	F,are
R5981	T8555	T8556	punct	-,J
R5982	T8556	T8554	prep	J,F
R5983	T8557	T8553	punct	),are
R5984	T8558	T8553	prep	from,are
R5985	T8559	T8560	nummod	54,wk
R5986	T8560	T8561	compound	wk,lenses
R5987	T8561	T8558	pobj	lenses,from
R5988	T8562	T8563	compound	alphaA,BKO
R5989	T8563	T8561	compound	BKO,lenses
R599	T1146	T1142	appos	alphaB,crystallin
R5990	T8564	T8563	punct	/,BKO
R5991	T8565	T8553	punct	.,are
R5992	T8567	T8568	punct	(,are
R5993	T8569	T8568	nsubj	K,are
R5994	T8570	T8571	punct	-,O
R5995	T8571	T8569	prep	O,K
R5996	T8572	T8568	punct	),are
R5997	T8573	T8568	prep	from,are
R5998	T8574	T8575	nummod	5,wk
R5999	T8575	T8576	nmod	wk,lenses
R60	T199	T198	punct	-,crystallin
R600	T1147	T1137	punct	", ",shown
R6000	T8576	T8573	pobj	lenses,from
R6001	T8577	T8578	amod	wild,type
R6002	T8578	T8576	compound	type,lenses
R6003	T8579	T8568	punct	.,are
R6004	T8581	T8582	punct	(,are
R6005	T8583	T8582	nsubj	P,are
R6006	T8584	T8585	punct	-,T
R6007	T8585	T8583	prep	T,P
R6008	T8586	T8582	punct	),are
R6009	T8587	T8582	prep	from,are
R601	T1148	T1137	auxpass	was,shown
R6010	T8588	T8589	nummod	46,wk
R6011	T8589	T8590	nmod	wk,lenses
R6012	T8590	T8587	pobj	lenses,from
R6013	T8591	T8592	amod	wild,type
R6014	T8592	T8590	compound	type,lenses
R6015	T8593	T8582	punct	.,are
R6016	T8595	T8596	punct	(,are
R6017	T8597	T8596	nsubj	A,are
R6018	T8598	T8597	punct	", ",A
R6019	T8599	T8597	conj	F,A
R602	T1149	T1150	aux	to,restricted
R6020	T8600	T8599	punct	", ",F
R6021	T8601	T8599	conj	K,F
R6022	T8602	T8601	punct	", ",K
R6023	T8603	T8601	cc	and,K
R6024	T8604	T8601	conj	P,K
R6025	T8605	T8596	punct	),are
R6026	T8606	T8607	amod	anterior,cortex
R6027	T8607	T8596	attr	cortex,are
R6028	T8608	T8607	amod	central,cortex
R6029	T8609	T8607	cc	and,cortex
R603	T1150	T1137	xcomp	restricted,shown
R6030	T8610	T8607	conj	epithelium,cortex
R6031	T8611	T8596	punct	.,are
R6032	T8613	T8614	punct	(,are
R6033	T8615	T8614	nsubj	B,are
R6034	T8616	T8615	punct	", ",B
R6035	T8617	T8615	conj	G,B
R6036	T8618	T8617	punct	", ",G
R6037	T8619	T8617	conj	L,G
R6038	T8620	T8619	punct	", ",L
R6039	T8621	T8619	cc	and,L
R604	T1151	T1150	neg	not,restricted
R6040	T8622	T8619	conj	Q,L
R6041	T8623	T8614	punct	),are
R6042	T8624	T8625	amod	equatorial,bow
R6043	T8625	T8626	compound	bow,region
R6044	T8626	T8614	attr	region,are
R6045	T8627	T8614	punct	.,are
R6046	T8629	T8630	punct	(,are
R6047	T8631	T8630	nsubj	C,are
R6048	T8632	T8631	punct	", ",C
R6049	T8633	T8631	conj	H,C
R605	T1152	T1150	auxpass	be,restricted
R6050	T8634	T8633	punct	", ",H
R6051	T8635	T8633	conj	M,H
R6052	T8636	T8635	punct	", ",M
R6053	T8637	T8635	cc	and,M
R6054	T8638	T8635	conj	R,M
R6055	T8639	T8630	punct	),are
R6056	T8640	T8641	amod	embryonic,fetal
R6057	T8641	T8643	amod	fetal,nucleus
R6058	T8642	T8641	punct	/,fetal
R6059	T8643	T8630	attr	nucleus,are
R606	T1153	T1150	prep	to,restricted
R6060	T8644	T8630	punct	.,are
R6061	T8646	T8647	punct	(,are
R6062	T8648	T8647	nsubj	D,are
R6063	T8649	T8648	punct	", ",D
R6064	T8650	T8648	conj	I,D
R6065	T8651	T8650	punct	", ",I
R6066	T8652	T8650	conj	N,I
R6067	T8653	T8652	punct	", ",N
R6068	T8654	T8652	cc	and,N
R6069	T8655	T8652	conj	S,N
R607	T1154	T1155	det	the,lens
R6070	T8656	T8647	punct	),are
R6071	T8657	T8658	amod	deep,cortex
R6072	T8658	T8647	attr	cortex,are
R6073	T8659	T8658	amod	anterior,cortex
R6074	T8660	T8647	punct	.,are
R6075	T8662	T8663	punct	(,are
R6076	T8664	T8663	nsubj	E,are
R6077	T8665	T8664	punct	", ",E
R6078	T8666	T8664	conj	J,E
R6079	T8667	T8666	punct	", ",J
R608	T1155	T1153	pobj	lens,to
R6080	T8668	T8666	conj	O,J
R6081	T8669	T8668	punct	", ",O
R6082	T8670	T8668	cc	and,O
R6083	T8671	T8668	conj	T,O
R6084	T8672	T8663	punct	),are
R6085	T8673	T8674	amod	posterior,region
R6086	T8674	T8663	attr	region,are
R6087	T8675	T8674	amod	subcapsular,region
R6088	T8676	T8663	punct	.,are
R6089	T8678	T8679	punct	(,indicates
R609	T1156	T1137	punct	.,shown
R6090	T8680	T8679	nsubj	*,indicates
R6091	T8681	T8679	punct	),indicates
R6092	T8682	T8679	dobj	areas,indicates
R6093	T8683	T8682	amod	devoid,areas
R6094	T8684	T8683	prep	of,devoid
R6095	T8685	T8686	amod	cellular,material
R6096	T8686	T8684	pobj	material,of
R6097	T8687	T8688	punct	(,B
R6098	T8688	T8679	parataxis	B,indicates
R6099	T8689	T8688	cc	and,B
R61	T200	T192	punct	", ",compensate
R610	T1158	T1159	nsubjpass	AlphaB,found
R6100	T8690	T8688	conj	G,B
R6101	T8691	T8688	punct	),B
R6102	T8692	T8679	punct	.,indicates
R6103	T8694	T8695	nsubj	Arrow,indicates
R6104	T8696	T8697	amod	posterior,suture
R6105	T8697	T8695	dobj	suture,indicates
R6106	T8698	T8699	punct	(,O
R6107	T8699	T8695	parataxis	O,indicates
R6108	T8700	T8699	punct	),O
R6109	T8701	T8695	punct	.,indicates
R611	T1160	T1159	auxpass	was,found
R6110	T8703	T8704	nsubj	Arrowheads,indicate
R6111	T8705	T8706	amod	representative,nuclei
R6112	T8706	T8704	dobj	nuclei,indicate
R6113	T8707	T8706	acl	stained,nuclei
R6114	T8708	T8707	prep	with,stained
R6115	T8709	T8710	compound	SYTOX,green
R6116	T8710	T8708	pobj	green,with
R6117	T8711	T8712	punct	(,A
R6118	T8712	T8704	parataxis	A,indicate
R6119	T8713	T8712	punct	", ",A
R612	T1161	T1162	aux	to,expressed
R6120	T8714	T8712	conj	B,A
R6121	T8715	T8714	punct	", ",B
R6122	T8716	T8714	conj	E,B
R6123	T8717	T8716	punct	", ",E
R6124	T8718	T8716	conj	F,E
R6125	T8719	T8718	punct	", ",F
R6126	T8720	T8718	conj	G,F
R6127	T8721	T8720	punct	", ",G
R6128	T8722	T8720	conj	I,G
R6129	T8723	T8722	punct	", ",I
R613	T1162	T1159	xcomp	expressed,found
R6130	T8724	T8722	conj	K,I
R6131	T8725	T8724	punct	", ",K
R6132	T8726	T8724	conj	L,K
R6133	T8727	T8726	punct	", ",L
R6134	T8728	T8726	conj	P,L
R6135	T8729	T8728	punct	", ",P
R6136	T8730	T8728	cc	and,P
R6137	T8731	T8728	conj	Q,P
R6138	T8732	T8712	punct	),A
R6139	T8733	T8704	punct	.,indicate
R614	T1163	T1162	auxpass	be,expressed
R6140	T8758	T8759	amod	Representative,micrographs
R6141	T8760	T8759	compound	scanning,micrographs
R6142	T8761	T8759	compound	electron,micrographs
R6143	T8762	T8759	prep	of,micrographs
R6144	T8763	T8764	nmod	alphaA,BKO
R6145	T8764	T8766	nmod	BKO,lenses
R6146	T8765	T8764	punct	/,BKO
R6147	T8766	T8762	pobj	lenses,of
R6148	T8767	T8768	punct	(,A
R6149	T8768	T8764	parataxis	A,BKO
R615	T1164	T1162	prep	at,expressed
R6150	T8769	T8770	punct	-,F
R6151	T8770	T8768	prep	F,A
R6152	T8771	T8768	punct	),A
R6153	T8772	T8764	cc	and,BKO
R6154	T8773	T8774	amod	wild,type
R6155	T8774	T8764	conj	type,BKO
R6156	T8775	T8776	punct	(,G
R6157	T8776	T8774	parataxis	G,type
R6158	T8777	T8778	punct	-,L
R6159	T8778	T8776	prep	L,G
R616	T1165	T1166	amod	significant,levels
R6160	T8779	T8776	punct	),G
R6161	T8780	T8759	punct	.,micrographs
R6162	T8782	T8783	punct	(,A
R6163	T8783	T8784	meta	A,images
R6164	T8785	T8783	punct	-,A
R6165	T8786	T8783	dep	C,A
R6166	T8787	T8783	punct	),A
R6167	T8788	T8784	amod	Representative,images
R6168	T8789	T8784	prep	from,images
R6169	T8790	T8791	nummod	5,wk
R617	T1166	T1164	pobj	levels,at
R6170	T8791	T8792	compound	wk,lenses
R6171	T8792	T8789	pobj	lenses,from
R6172	T8793	T8794	compound	alphaA,BKO
R6173	T8794	T8792	compound	BKO,lenses
R6174	T8795	T8794	punct	/,BKO
R6175	T8796	T8784	punct	.,images
R6176	T8798	T8799	punct	(,D
R6177	T8799	T8800	meta	D,images
R6178	T8801	T8799	punct	-,D
R6179	T8802	T8799	dep	F,D
R618	T1167	T1162	prep	in,expressed
R6180	T8803	T8799	punct	),D
R6181	T8804	T8800	amod	Representative,images
R6182	T8805	T8800	prep	from,images
R6183	T8806	T8807	nummod	54,wk
R6184	T8807	T8808	compound	wk,lenses
R6185	T8808	T8805	pobj	lenses,from
R6186	T8809	T8810	compound	alphaA,BKO
R6187	T8810	T8808	compound	BKO,lenses
R6188	T8811	T8810	punct	/,BKO
R6189	T8812	T8800	punct	.,images
R619	T1168	T1169	det	a,variety
R6190	T8814	T8815	punct	(,G
R6191	T8815	T8816	meta	G,images
R6192	T8817	T8815	punct	-,G
R6193	T8818	T8815	dep	I,G
R6194	T8819	T8815	punct	),G
R6195	T8820	T8816	amod	Representative,images
R6196	T8821	T8816	prep	from,images
R6197	T8822	T8823	nummod	5,wk
R6198	T8823	T8824	nmod	wk,lenses
R6199	T8824	T8821	pobj	lenses,from
R62	T201	T202	det	the,crystallin
R620	T1169	T1167	pobj	variety,in
R6200	T8825	T8826	amod	wild,type
R6201	T8826	T8824	compound	type,lenses
R6202	T8827	T8816	punct	.,images
R6203	T8829	T8830	punct	(,J
R6204	T8830	T8831	meta	J,images
R6205	T8832	T8830	punct	-,J
R6206	T8833	T8830	dep	L,J
R6207	T8834	T8830	punct	),J
R6208	T8835	T8831	amod	Representative,images
R6209	T8836	T8831	prep	from,images
R621	T1170	T1169	prep	of,variety
R6210	T8837	T8838	nummod	46,wk
R6211	T8838	T8839	nmod	wk,lenses
R6212	T8839	T8836	pobj	lenses,from
R6213	T8840	T8841	amod	wild,type
R6214	T8841	T8839	compound	type,lenses
R6215	T8842	T8831	punct	.,images
R6216	T8844	T8845	punct	(,A
R6217	T8845	T8846	meta	A,region
R6218	T8847	T8845	punct	", ",A
R6219	T8848	T8845	conj	D,A
R622	T1171	T1172	amod	nonlenticular,tissues
R6220	T8849	T8848	punct	", ",D
R6221	T8850	T8848	conj	G,D
R6222	T8851	T8850	punct	", ",G
R6223	T8852	T8850	cc	and,G
R6224	T8853	T8850	conj	J,G
R6225	T8854	T8853	punct	),J
R6226	T8855	T8856	amod	Equatorial,bow
R6227	T8856	T8846	amod	bow,region
R6228	T8857	T8856	punct	/,bow
R6229	T8858	T8846	punct	.,region
R623	T1172	T1170	pobj	tissues,of
R6230	T8860	T8861	punct	(,B
R6231	T8861	T8862	meta	B,cortex
R6232	T8863	T8861	punct	", ",B
R6233	T8864	T8861	conj	E,B
R6234	T8865	T8864	punct	", ",E
R6235	T8866	T8864	conj	H,E
R6236	T8867	T8866	punct	", ",H
R6237	T8868	T8866	cc	and,H
R6238	T8869	T8866	conj	K,H
R6239	T8870	T8869	punct	),K
R624	T1173	T1159	punct	", ",found
R6240	T8871	T8862	amod	Anterior,cortex
R6241	T8872	T8862	punct	.,cortex
R6242	T8874	T8875	meta	C,region
R6243	T8876	T8874	punct	", ",C
R6244	T8877	T8878	punct	(,F
R6245	T8878	T8874	conj	F,C
R6246	T8879	T8878	punct	", ",F
R6247	T8880	T8878	conj	I,F
R6248	T8881	T8880	punct	", ",I
R6249	T8882	T8880	cc	and,I
R625	T1174	T1175	mark	while,detected
R6250	T8883	T8880	conj	L,I
R6251	T8884	T8883	punct	),L
R6252	T8885	T8875	amod	Posterior,region
R6253	T8886	T8875	amod	subcapsular,region
R6254	T8887	T8875	punct	.,region
R6255	T8891	T8890	cc	and,Dimensions
R6256	T8892	T8893	amod	gross,appearance
R6257	T8893	T8890	conj	appearance,Dimensions
R6258	T8894	T8893	amod	lenticular,appearance
R6259	T8895	T8890	prep	of,Dimensions
R626	T1175	T1159	advcl	detected,found
R6260	T8896	T8895	pobj	lenses,of
R6261	T8897	T8890	punct	.,Dimensions
R627	T1176	T1175	nsubjpass	alphaA,detected
R628	T1177	T1175	aux	has,detected
R629	T1178	T1175	advmod	only,detected
R63	T202	T192	nsubj	crystallin,compensate
R630	T1179	T1175	auxpass	been,detected
R631	T1180	T1175	prep	in,detected
R632	T1181	T1182	amod	small,amounts
R633	T1182	T1180	pobj	amounts,in
R634	T1183	T1175	prep	in,detected
R635	T1184	T1185	det	a,tissues
R636	T1185	T1183	pobj	tissues,in
R637	T1186	T1185	amod	few,tissues
R638	T1187	T1185	amod	other,tissues
R639	T1188	T1189	amod	such,as
R64	T203	T202	amod	remaining,crystallin
R640	T1189	T1185	prep	as,tissues
R641	T1190	T1189	pobj	retina,as
R642	T1191	T1190	punct	", ",retina
R643	T1192	T1190	conj	spleen,retina
R644	T1193	T1192	cc	and,spleen
R645	T1194	T1192	conj	thymus,spleen
R646	T1195	T1196	punct	[,8
R647	T1196	T1159	parataxis	8,found
R648	T1197	T1198	punct	-,12
R649	T1198	T1196	prep	12,8
R65	T204	T202	compound	alpha,crystallin
R650	T1199	T1196	punct	],8
R651	T1200	T1159	punct	.,found
R652	T1202	T1203	advmod	Collectively,challenged
R653	T1204	T1203	punct	", ",challenged
R654	T1205	T1206	det	these,findings
R655	T1206	T1203	nsubj	findings,challenged
R656	T1207	T1203	aux	have,challenged
R657	T1208	T1209	det	the,dogma
R658	T1209	T1203	dobj	dogma,challenged
R659	T1210	T1211	mark	that,is
R66	T205	T202	punct	-,crystallin
R660	T1211	T1209	acl	is,dogma
R661	T1212	T1213	compound	alpha,crystallin
R662	T1213	T1211	nsubj	crystallin,is
R663	T1214	T1213	punct	-,crystallin
R664	T1215	T1211	advmod	purely,is
R665	T1216	T1217	det	a,protein
R666	T1217	T1211	attr	protein,is
R667	T1218	T1217	amod	structural,protein
R668	T1219	T1217	amod	necessary,protein
R669	T1220	T1219	prep	for,necessary
R67	T206	T192	aux	may,compensate
R670	T1221	T1222	amod	light,refraction
R671	T1222	T1220	pobj	refraction,for
R672	T1223	T1203	punct	", ",challenged
R673	T1224	T1203	cc	and,challenged
R674	T1225	T1226	aux	have,led
R675	T1226	T1203	conj	led,challenged
R676	T1227	T1226	prep	to,led
R677	T1228	T1229	det	the,realization
R678	T1229	T1227	pobj	realization,to
R679	T1230	T1231	mark	that,have
R68	T207	T192	advmod	fully,compensate
R680	T1231	T1229	acl	have,realization
R681	T1232	T1233	compound	alpha,crystallin
R682	T1233	T1231	nsubj	crystallin,have
R683	T1234	T1233	punct	-,crystallin
R684	T1235	T1231	aux	may,have
R685	T1236	T1237	det	a,variety
R686	T1237	T1231	dobj	variety,have
R687	T1238	T1237	prep	of,variety
R688	T1239	T1240	amod	biological,functions
R689	T1240	T1238	pobj	functions,of
R69	T208	T207	cc	or,fully
R690	T1241	T1231	prep	in,have
R691	T1242	T1243	det	the,lens
R692	T1243	T1241	pobj	lens,in
R693	T1244	T1203	punct	.,challenged
R694	T1246	T1247	det	A,scope
R695	T1247	T1250	nsubjpass	scope,inferred
R696	T1248	T1249	advmod	much,broader
R697	T1249	T1247	amod	broader,scope
R698	T1251	T1247	prep	of,scope
R699	T1252	T1253	amod	cellular,functions
R70	T209	T207	conj	partially,fully
R700	T1253	T1251	pobj	functions,of
R701	T1254	T1253	prep	of,functions
R702	T1255	T1256	compound	alpha,crystallin
R703	T1256	T1254	pobj	crystallin,of
R704	T1257	T1256	punct	-,crystallin
R705	T1258	T1253	prep	in,functions
R706	T1259	T1258	pobj	lens,in
R707	T1260	T1250	auxpass	is,inferred
R708	T1261	T1250	prep	from,inferred
R709	T1262	T1263	advmod	in,vitro
R71	T210	T192	prep	for,compensate
R710	T1263	T1264	amod	vitro,observations
R711	T1264	T1261	pobj	observations,from
R712	T1265	T1250	punct	.,inferred
R713	T1267	T1268	preconj	Both,alphaA
R714	T1268	T1269	nsubj	alphaA,bind
R715	T1270	T1268	cc	and,alphaA
R716	T1271	T1268	conj	alphaB,alphaA
R717	T1272	T1269	aux	can,bind
R718	T1273	T1269	advmod	specifically,bind
R719	T1274	T1269	prep	to,bind
R72	T211	T210	pobj	some,for
R720	T1275	T1274	pobj	actin,to
R721	T1276	T1269	punct	", ",bind
R722	T1277	T1278	advmod	in,13
R723	T1278	T1269	advmod	13,bind
R724	T1279	T1278	advmod	vitro,13
R725	T1280	T1278	punct	[,13
R726	T1281	T1278	punct	],13
R727	T1282	T1278	cc	and,13
R728	T1283	T1284	advmod	in,14
R729	T1284	T1278	conj	14,13
R73	T212	T211	prep	of,some
R730	T1285	T1284	advmod	vivo,14
R731	T1286	T1284	punct	[,14
R732	T1287	T1284	punct	],14
R733	T1288	T1269	punct	.,bind
R734	T1290	T1291	mark	Although,shown
R735	T1291	T1297	advcl	shown,known
R736	T1292	T1293	compound	actin,filament
R737	T1293	T1294	compound	filament,formation
R738	T1294	T1291	nsubjpass	formation,shown
R739	T1295	T1291	aux	has,shown
R74	T213	T214	det	the,functions
R740	T1296	T1291	auxpass	been,shown
R741	T1298	T1299	aux	to,be
R742	T1299	T1291	xcomp	be,shown
R743	T1300	T1299	acomp	necessary,be
R744	T1301	T1300	prep	for,necessary
R745	T1302	T1301	pobj	differentiation,for
R746	T1303	T1302	prep	of,differentiation
R747	T1304	T1305	nmod	lens,cells
R748	T1305	T1303	pobj	cells,of
R749	T1306	T1305	amod	epithelial,cells
R75	T214	T212	pobj	functions,of
R750	T1307	T1308	punct	[,15
R751	T1308	T1291	parataxis	15,shown
R752	T1309	T1308	punct	],15
R753	T1310	T1297	punct	", ",known
R754	T1311	T1312	det	the,significance
R755	T1312	T1297	nsubjpass	significance,known
R756	T1313	T1312	prep	of,significance
R757	T1314	T1315	compound	alpha,crystallin
R758	T1315	T1317	poss	crystallin,interaction
R759	T1316	T1315	punct	-,crystallin
R76	T215	T214	prep	of,functions
R760	T1317	T1313	pobj	interaction,of
R761	T1318	T1315	case	's,crystallin
R762	T1319	T1317	prep	with,interaction
R763	T1320	T1319	pobj	actin,with
R764	T1321	T1317	prep	in,interaction
R765	T1322	T1321	pobj	differentiation,in
R766	T1323	T1297	auxpass	is,known
R767	T1324	T1297	neg	not,known
R768	T1325	T1297	punct	.,known
R769	T1327	T1328	prep	In,associates
R77	T216	T217	det	the,protein
R770	T1329	T1330	det	the,lens
R771	T1330	T1327	pobj	lens,In
R772	T1331	T1328	punct	", ",associates
R773	T1332	T1333	compound	alpha,crystallin
R774	T1333	T1328	nsubj	crystallin,associates
R775	T1334	T1333	punct	-,crystallin
R776	T1335	T1328	advmod	also,associates
R777	T1336	T1328	prep	with,associates
R778	T1337	T1338	nmod	type,proteins
R779	T1338	T1336	pobj	proteins,with
R78	T217	T215	pobj	protein,of
R780	T1339	T1337	nummod	III,type
R781	T1340	T1341	amod	intermediate,filament
R782	T1341	T1338	compound	filament,proteins
R783	T1342	T1338	cc	and,proteins
R784	T1343	T1344	det	the,proteins
R785	T1344	T1338	conj	proteins,proteins
R786	T1345	T1346	amod	beaded,filament
R787	T1346	T1344	compound	filament,proteins
R788	T1347	T1344	appos	CP49,proteins
R789	T1348	T1347	cc	and,CP49
R79	T218	T217	amod	missing,protein
R790	T1349	T1347	conj	CP115,CP49
R791	T1350	T1328	punct	", ",associates
R792	T1351	T1328	cc	and,associates
R793	T1352	T1353	amod	correct,assembly
R794	T1353	T1356	nsubjpass	assembly,shown
R795	T1354	T1355	amod	beaded,filament
R796	T1355	T1353	compound	filament,assembly
R797	T1356	T1328	conj	shown,associates
R798	T1357	T1356	aux	has,shown
R799	T1358	T1356	auxpass	been,shown
R8	T140	T138	prep	of,characterization
R80	T219	T192	punct	", ",compensate
R800	T1359	T1356	xcomp	depend,shown
R801	T1360	T1359	prep	on,depend
R802	T1361	T1362	det	the,presence
R803	T1362	T1360	pobj	presence,on
R804	T1363	T1362	prep	of,presence
R805	T1364	T1365	compound	alpha,crystallin
R806	T1365	T1363	pobj	crystallin,of
R807	T1366	T1365	punct	-,crystallin
R808	T1367	T1368	punct	[,16
R809	T1368	T1356	parataxis	16,shown
R81	T220	T221	advmod	especially,in
R810	T1369	T1368	punct	],16
R811	T1370	T1328	punct	.,associates
R812	T1372	T1373	amod	Beaded,filament
R813	T1373	T1374	compound	filament,levels
R814	T1374	T1376	nsubjpass	levels,increased
R815	T1375	T1374	compound	mRNA,levels
R816	T1377	T1376	auxpass	are,increased
R817	T1378	T1376	advmod	greatly,increased
R818	T1379	T1376	prep	in,increased
R819	T1380	T1381	amod	differentiating,epithelium
R82	T221	T192	prep	in,compensate
R820	T1381	T1379	pobj	epithelium,in
R821	T1382	T1381	compound	lens,epithelium
R822	T1383	T1376	cc	and,increased
R823	T1384	T1385	aux	have,suggested
R824	T1385	T1376	conj	suggested,increased
R825	T1386	T1385	auxpass	been,suggested
R826	T1387	T1385	prep	as,suggested
R827	T1388	T1389	det	a,marker
R828	T1389	T1387	pobj	marker,as
R829	T1390	T1389	amod	pan-specific,marker
R83	T222	T223	det	the,lens
R830	T1391	T1389	prep	for,marker
R831	T1392	T1393	compound	lens,fiber
R832	T1393	T1394	compound	fiber,development
R833	T1394	T1391	pobj	development,for
R834	T1395	T1396	punct	[,17
R835	T1396	T1376	parataxis	17,increased
R836	T1397	T1396	punct	],17
R837	T1398	T1376	punct	.,increased
R838	T1400	T1401	compound	Alpha,crystallin
R839	T1401	T1403	nsubjpass	crystallin,shown
R84	T223	T221	pobj	lens,in
R840	T1402	T1401	punct	-,crystallin
R841	T1404	T1403	aux	has,shown
R842	T1405	T1403	advmod	also,shown
R843	T1406	T1403	auxpass	been,shown
R844	T1407	T1408	aux	to,interact
R845	T1408	T1403	xcomp	interact,shown
R846	T1409	T1408	advmod	directly,interact
R847	T1410	T1408	prep	with,interact
R848	T1411	T1410	pobj	DNA,with
R849	T1412	T1413	punct	[,18
R85	T224	T223	punct	", ",lens
R850	T1413	T1403	parataxis	18,shown
R851	T1414	T1413	punct	],18
R852	T1415	T1403	punct	.,shown
R853	T1417	T1418	prep	In,shown
R854	T1419	T1420	amod	transfected,cells
R855	T1420	T1417	pobj	cells,In
R856	T1421	T1420	compound	CHO,cells
R857	T1422	T1418	punct	", ",shown
R858	T1423	T1418	nsubjpass	alphaB,shown
R859	T1424	T1418	aux	has,shown
R86	T225	T226	advmod	where,expressed
R860	T1425	T1418	advmod	also,shown
R861	T1426	T1418	auxpass	been,shown
R862	T1427	T1428	aux	to,localize
R863	T1428	T1418	xcomp	localize,shown
R864	T1429	T1428	advmod	ectopically,localize
R865	T1430	T1428	prep	to,localize
R866	T1431	T1432	compound	interphase,nuclei
R867	T1432	T1430	pobj	nuclei,to
R868	T1433	T1428	punct	", ",localize
R869	T1434	T1428	advcl	suggesting,localize
R87	T226	T223	relcl	expressed,lens
R870	T1435	T1436	det	a,role
R871	T1436	T1434	dobj	role,suggesting
R872	T1437	T1436	prep	for,role
R873	T1438	T1439	det	this,protein
R874	T1439	T1437	pobj	protein,for
R875	T1440	T1436	prep	in,role
R876	T1441	T1442	det	the,nucleus
R877	T1442	T1440	pobj	nucleus,in
R878	T1443	T1444	punct	[,19
R879	T1444	T1418	parataxis	19,shown
R88	T227	T228	preconj	both,alphaA
R880	T1445	T1444	punct	],19
R881	T1446	T1418	punct	.,shown
R882	T1448	T1449	det	A,role
R883	T1449	T1451	nsubjpass	role,supported
R884	T1450	T1449	amod	nuclear,role
R885	T1452	T1449	prep	for,role
R886	T1453	T1452	pobj	alphaB,for
R887	T1454	T1449	prep	in,role
R888	T1455	T1456	det	the,lens
R889	T1456	T1454	pobj	lens,in
R89	T228	T226	nsubjpass	alphaA,expressed
R890	T1457	T1451	auxpass	was,supported
R891	T1458	T1451	agent	by,supported
R892	T1459	T1460	det	the,findings
R893	T1460	T1458	pobj	findings,by
R894	T1461	T1462	mark	that,demonstrated
R895	T1462	T1460	acl	demonstrated,findings
R896	T1463	T1464	det	a,subset
R897	T1464	T1462	nsubj	subset,demonstrated
R898	T1465	T1464	prep	of,subset
R899	T1466	T1467	nmod	lens,cells
R9	T141	T142	det	the,lens
R90	T229	T228	cc	and,alphaA
R900	T1467	T1465	pobj	cells,of
R901	T1468	T1467	amod	epithelial,cells
R902	T1469	T1467	acl	derived,cells
R903	T1470	T1469	prep	from,derived
R904	T1471	T1472	compound	alphaB,mice
R905	T1472	T1470	pobj	mice,from
R906	T1473	T1472	compound	knockout,mice
R907	T1474	T1462	dobj	hyperproliferation,demonstrated
R908	T1475	T1474	cc	and,hyperproliferation
R909	T1476	T1477	amod	genomic,instability
R91	T230	T228	conj	alphaB,alphaA
R910	T1477	T1474	conj	instability,hyperproliferation
R911	T1478	T1479	punct	[,20
R912	T1479	T1451	parataxis	20,supported
R913	T1480	T1479	punct	],20
R914	T1481	T1451	punct	.,supported
R915	T1483	T1484	prep	In,resulted
R916	T1485	T1483	pobj	addition,In
R917	T1486	T1484	punct	", ",resulted
R918	T1487	T1488	det	the,administration
R919	T1488	T1484	nsubj	administration,resulted
R92	T231	T226	auxpass	are,expressed
R920	T1489	T1488	prep	of,administration
R921	T1490	T1491	amod	exogenous,crystallin
R922	T1491	T1489	pobj	crystallin,of
R923	T1492	T1491	compound	alpha,crystallin
R924	T1493	T1491	punct	-,crystallin
R925	T1494	T1488	prep	to,administration
R926	T1495	T1496	amod	primary,cultures
R927	T1496	T1494	pobj	cultures,to
R928	T1497	T1498	amod	bovine,lens
R929	T1498	T1499	nmod	lens,cell
R93	T232	T226	advmod	normally,expressed
R930	T1499	T1496	compound	cell,cultures
R931	T1500	T1499	amod	epithelial,cell
R932	T1501	T1484	prep	in,resulted
R933	T1502	T1503	det	the,formation
R934	T1503	T1501	pobj	formation,in
R935	T1504	T1503	prep	of,formation
R936	T1505	T1506	amod	lentoid,bodies
R937	T1506	T1504	pobj	bodies,of
R938	T1507	T1484	punct	", ",resulted
R939	T1508	T1484	advcl	consistent,resulted
R94	T233	T226	prep	at,expressed
R940	T1509	T1508	prep	with,consistent
R941	T1510	T1511	det	a,role
R942	T1511	T1509	pobj	role,with
R943	T1512	T1511	prep	for,role
R944	T1513	T1514	det	these,proteins
R945	T1514	T1512	pobj	proteins,for
R946	T1515	T1511	prep	in,role
R947	T1516	T1517	compound	lens,differentiation
R948	T1517	T1515	pobj	differentiation,in
R949	T1518	T1519	punct	[,21
R95	T234	T235	amod	high,levels
R950	T1519	T1484	parataxis	21,resulted
R951	T1520	T1519	punct	],21
R952	T1521	T1484	punct	.,resulted
R953	T1523	T1524	det	These,findings
R954	T1524	T1525	nsubj	findings,indicate
R955	T1526	T1527	mark	that,have
R956	T1609	T1608	prep	of,solubility
R957	T1527	T1525	ccomp	have,indicate
R958	T1610	T1609	pobj	alphaB,of
R959	T1528	T1529	compound	alpha,crystallin
R96	T235	T233	pobj	levels,at
R960	T1611	T1610	punct	", ",alphaB
R961	T1612	T1610	cc	or,alphaB
R962	T1613	T1614	amod	associated,proteins
R963	T1529	T1527	nsubj	crystallin,have
R964	T1614	T1610	conj	proteins,alphaB
R965	T1615	T1608	punct	", ",solubility
R966	T1616	T1608	prep	in,solubility
R967	T1617	T1618	det	the,lens
R968	T1618	T1616	pobj	lens,in
R969	T1530	T1529	punct	-,crystallin
R97	T236	T192	punct	.,compensate
R970	T1619	T1576	punct	.,demonstrated
R971	T1621	T1622	det	These,lenses
R972	T1531	T1527	aux	may,have
R973	T1622	T1623	nsubjpass	lenses,reported
R974	T1532	T1533	det	a,multitude
R975	T1624	T1623	auxpass	were,reported
R976	T1625	T1623	advmod	also,reported
R977	T1626	T1627	aux	to,be
R978	T1533	T1527	dobj	multitude,have
R979	T1627	T1623	xcomp	be,reported
R98	T238	T239	det	The,purpose
R980	T1628	T1627	acomp	smaller,be
R981	T1629	T1628	prep	in,smaller
R982	T1630	T1631	amod	equatorial,dimensions
R983	T1534	T1533	prep	of,multitude
R984	T1631	T1629	pobj	dimensions,in
R985	T1632	T1630	cc	and,equatorial
R986	T1633	T1634	amod	light,axial
R987	T1535	T1536	advmod	in,vivo
R988	T1634	T1630	conj	axial,equatorial
R989	T1635	T1628	prep	than,smaller
R99	T239	T240	nsubj	purpose,was
R990	T1536	T1537	amod	vivo,functions
R991	T1636	T1637	npadvmod	age,matched
R992	T1637	T1638	amod	matched,lens
R993	T1638	T1635	pobj	lens,than
R994	T1537	T1534	pobj	functions,of
R995	T1639	T1640	amod	wild,type
R996	T1640	T1638	compound	type,lens
R997	T1538	T1525	punct	.,indicate
R998	T1641	T1623	punct	.,reported
R999	T1643	T1644	nsubj	It,was

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PMC:149350 JSON TXT 7 Projects