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PMC:1482699 / 33845-45803 JSONTXT 4 Projects

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Id Subject Object Predicate Lexical cue
T20228 9-14 NN denotes Mouse
T20229 15-22 NNS denotes strains
T20230 23-26 CC denotes and
T20231 27-36 JJ denotes husbandry
T20232 36-110 sentence denotes CAST/EiJ (stock #000928) mice were purchased from the Jackson Laboratory.
T20233 37-41 NN denotes CAST
T20235 41-42 HYPH denotes /
T20234 42-45 NN denotes EiJ
T20237 46-47 -LRB- denotes (
T20239 47-52 NN denotes stock
T20238 53-54 NN denotes #
T20240 54-60 CD denotes 000928
T20241 60-61 -RRB- denotes )
T20236 62-66 NNS denotes mice
T20243 67-71 VBD denotes were
T20242 72-81 VBN denotes purchased
T20244 82-86 IN denotes from
T20245 87-90 DT denotes the
T20247 91-98 NNP denotes Jackson
T20246 99-109 NNP denotes Laboratory
T20248 109-110 . denotes .
T20249 110-278 sentence denotes The hg mutation was originally identified in a selected outcross line [22] and has been introgressed onto a B6 background via nine backcrosses to create the HG strain.
T20250 111-114 DT denotes The
T20252 115-117 NN denotes hg
T20251 118-126 NN denotes mutation
T20254 127-130 VBD denotes was
T20255 131-141 RB denotes originally
T20253 142-152 VBN denotes identified
T20256 153-155 IN denotes in
T20257 156-157 DT denotes a
T20259 158-166 VBN denotes selected
T20260 167-175 NN denotes outcross
T20258 176-180 NN denotes line
T20261 181-182 -LRB- denotes [
T20262 182-184 CD denotes 22
T20263 184-185 -RRB- denotes ]
T20264 186-189 CC denotes and
T20265 190-193 VBZ denotes has
T20267 194-198 VBN denotes been
T20266 199-211 VBN denotes introgressed
T20268 212-216 IN denotes onto
T20269 217-218 DT denotes a
T20271 219-221 NN denotes B6
T20270 222-232 NN denotes background
T20272 233-236 IN denotes via
T20273 237-241 CD denotes nine
T20274 242-253 NNS denotes backcrosses
T20275 254-256 TO denotes to
T20276 257-263 VB denotes create
T20277 264-267 DT denotes the
T20279 268-270 NN denotes HG
T20278 271-277 NN denotes strain
T20280 277-278 . denotes .
T20281 278-364 sentence denotes HG mice used in this experiment were from the 17th or later generation of inbreeding.
T20282 279-281 NN denotes HG
T20283 282-286 NNS denotes mice
T20285 287-291 VBN denotes used
T20286 292-294 IN denotes in
T20287 295-299 DT denotes this
T20288 300-310 NN denotes experiment
T20284 311-315 VBD denotes were
T20289 316-320 IN denotes from
T20290 321-324 DT denotes the
T20292 325-329 JJ denotes 17th
T20293 330-332 CC denotes or
T20294 333-338 JJ denotes later
T20291 339-349 NN denotes generation
T20295 350-352 IN denotes of
T20296 353-363 NN denotes inbreeding
T20297 363-364 . denotes .
T20298 364-658 sentence denotes Mice were housed in polycarbonate cages under controlled conditions of temperature (21°C ± 2°C), humidity (40–70%) and lighting (14 h light, 10 h dark, lights on at 7 AM), and managed according to the guidelines of the American Association for Accreditation of Laboratory Animal Care (AAALAC).
T20299 365-369 NNS denotes Mice
T20301 370-374 VBD denotes were
T20300 375-381 VBN denotes housed
T20302 382-384 IN denotes in
T20303 385-398 NN denotes polycarbonate
T20304 399-404 NNS denotes cages
T20305 405-410 IN denotes under
T20306 411-421 VBN denotes controlled
T20307 422-432 NNS denotes conditions
T20308 433-435 IN denotes of
T20309 436-447 NN denotes temperature
T20310 448-449 -LRB- denotes (
T20312 449-451 CD denotes 21
T20311 451-453 NN denotes °C
T20313 454-455 SYM denotes ±
T20314 456-457 CD denotes 2
T20315 457-459 NN denotes °C
T20316 459-460 -RRB- denotes )
T20317 460-462 , denotes ,
T20318 462-470 NN denotes humidity
T20319 471-472 -LRB- denotes (
T20321 472-474 CD denotes 40
T20323 474-475 SYM denotes –
T20322 475-477 CD denotes 70
T20320 477-478 NN denotes %
T20324 478-479 -RRB- denotes )
T20325 480-483 CC denotes and
T20326 484-492 NN denotes lighting
T20327 493-494 -LRB- denotes (
T20329 494-496 CD denotes 14
T20330 497-498 NN denotes h
T20331 499-504 NN denotes light
T20332 504-506 , denotes ,
T20333 506-508 CD denotes 10
T20334 509-510 NN denotes h
T20328 511-515 NN denotes dark
T20335 515-517 , denotes ,
T20336 517-523 NNS denotes lights
T20337 524-526 RB denotes on
T20338 527-529 IN denotes at
T20339 530-531 CD denotes 7
T20340 532-534 NN denotes AM
T20341 534-535 -RRB- denotes )
T20342 535-537 , denotes ,
T20343 537-540 CC denotes and
T20344 541-548 VBD denotes managed
T20345 549-558 VBG denotes according
T20346 559-561 IN denotes to
T20347 562-565 DT denotes the
T20348 566-576 NNS denotes guidelines
T20349 577-579 IN denotes of
T20350 580-583 DT denotes the
T20352 584-592 NNP denotes American
T20351 593-604 NNP denotes Association
T20353 605-608 IN denotes for
T20354 609-622 NNP denotes Accreditation
T20355 623-625 IN denotes of
T20356 626-636 NNP denotes Laboratory
T20357 637-643 NNP denotes Animal
T20358 644-648 NNP denotes Care
T20359 649-650 -LRB- denotes (
T20360 650-656 NNP denotes AAALAC
T20361 656-657 -RRB- denotes )
T20362 657-658 . denotes .
T20779 660-670 NN denotes Genotyping
T20780 670-874 sentence denotes DNA for genotyping was isolated from 1.0–2.0 mm tail clips by digesting with Proteinase K (Fisher) at 55°C in a buffer composed of 0.45% NP40 (Sigma), 0.45% Tween 20 (Fisher) and 1X PCR buffer (Promega).
T20781 671-674 NN denotes DNA
T20783 675-678 IN denotes for
T20784 679-689 NN denotes genotyping
T20785 690-693 VBD denotes was
T20782 694-702 VBN denotes isolated
T20786 703-707 IN denotes from
T20787 708-711 CD denotes 1.0
T20789 711-712 SYM denotes –
T20788 712-715 CD denotes 2.0
T20790 716-718 NN denotes mm
T20792 719-723 NN denotes tail
T20791 724-729 NNS denotes clips
T20793 730-732 IN denotes by
T20794 733-742 VBG denotes digesting
T20795 743-747 IN denotes with
T20796 748-758 NN denotes Proteinase
T20797 759-760 NN denotes K
T20798 761-762 -LRB- denotes (
T20799 762-768 NNP denotes Fisher
T20800 768-769 -RRB- denotes )
T20801 770-772 IN denotes at
T20802 773-775 CD denotes 55
T20803 775-777 NN denotes °C
T20804 778-780 IN denotes in
T20805 781-782 DT denotes a
T20806 783-789 NN denotes buffer
T20807 790-798 VBN denotes composed
T20808 799-801 IN denotes of
T20809 802-806 CD denotes 0.45
T20810 806-807 NN denotes %
T20811 808-812 NN denotes NP40
T20812 813-814 -LRB- denotes (
T20813 814-819 NNP denotes Sigma
T20814 819-820 -RRB- denotes )
T20815 820-822 , denotes ,
T20816 822-826 CD denotes 0.45
T20817 826-827 NN denotes %
T20818 828-833 NN denotes Tween
T20819 834-836 CD denotes 20
T20820 837-838 -LRB- denotes (
T20821 838-844 NNP denotes Fisher
T20822 844-845 -RRB- denotes )
T20823 846-849 CC denotes and
T20824 850-852 NN denotes 1X
T20826 853-856 NN denotes PCR
T20825 857-863 NN denotes buffer
T20827 864-865 -LRB- denotes (
T20828 865-872 NNP denotes Promega
T20829 872-873 -RRB- denotes )
T20830 873-874 . denotes .
T20831 874-992 sentence denotes The product of this digestion was diluted (1:10) in sterile H2O and used for genotyping without further purification.
T20832 875-878 DT denotes The
T20833 879-886 NN denotes product
T20835 887-889 IN denotes of
T20836 890-894 DT denotes this
T20837 895-904 NN denotes digestion
T20838 905-908 VBD denotes was
T20834 909-916 VBN denotes diluted
T20839 917-918 -LRB- denotes (
T20840 918-919 CD denotes 1
T20841 919-920 SYM denotes :
T20842 920-922 CD denotes 10
T20843 922-923 -RRB- denotes )
T20844 924-926 IN denotes in
T20845 927-934 JJ denotes sterile
T20846 935-938 NN denotes H2O
T20847 939-942 CC denotes and
T20848 943-947 VBN denotes used
T20849 948-951 IN denotes for
T20850 952-962 NN denotes genotyping
T20851 963-970 IN denotes without
T20852 971-978 JJ denotes further
T20853 979-991 NN denotes purification
T20854 991-992 . denotes .
T20855 992-1086 sentence denotes Microsatellite genotyping was performed using standard PCR and gel electrophoresis protocols.
T20856 993-1007 NN denotes Microsatellite
T20857 1008-1018 NN denotes genotyping
T20859 1019-1022 VBD denotes was
T20858 1023-1032 VBN denotes performed
T20860 1033-1038 VBG denotes using
T20861 1039-1047 JJ denotes standard
T20863 1048-1051 NN denotes PCR
T20864 1052-1055 CC denotes and
T20865 1056-1059 NN denotes gel
T20866 1060-1075 NN denotes electrophoresis
T20862 1076-1085 NNS denotes protocols
T20867 1085-1086 . denotes .
T20868 1086-1161 sentence denotes Reaction conditions for each marker are listed in Additional File 1 and 2.
T20869 1087-1095 NN denotes Reaction
T20870 1096-1106 NNS denotes conditions
T20872 1107-1110 IN denotes for
T20873 1111-1115 DT denotes each
T20874 1116-1122 NN denotes marker
T20875 1123-1126 VBP denotes are
T20871 1127-1133 VBN denotes listed
T20876 1134-1136 IN denotes in
T20877 1137-1147 JJ denotes Additional
T20879 1148-1152 NN denotes File
T20878 1153-1154 CD denotes 1
T20880 1155-1158 CC denotes and
T20881 1159-1160 CD denotes 2
T20882 1160-1161 . denotes .
T20883 1161-1239 sentence denotes MMU2 congenic mice were genotyped for hg using a two primer genotyping assay.
T20884 1162-1166 NN denotes MMU2
T20885 1167-1175 JJ denotes congenic
T20886 1176-1180 NNS denotes mice
T20888 1181-1185 VBD denotes were
T20887 1186-1195 VBN denotes genotyped
T20889 1196-1199 IN denotes for
T20890 1200-1202 NN denotes hg
T20891 1203-1208 VBG denotes using
T20892 1209-1210 DT denotes a
T20894 1211-1214 CD denotes two
T20895 1215-1221 NN denotes primer
T20896 1222-1232 NN denotes genotyping
T20893 1233-1238 NN denotes assay
T20897 1238-1239 . denotes .
T20898 1239-1388 sentence denotes One primer set (HG-F, ctcctgtctgggctgtgag and HG-R, caaaggcagaagtggggtaa) spanned the hg deletion producing a 447 bp product in hg/hg and +/hg mice.
T20899 1240-1243 CD denotes One
T20901 1244-1250 NN denotes primer
T20900 1251-1254 NN denotes set
T20903 1255-1256 -LRB- denotes (
T20904 1256-1258 NN denotes HG
T20906 1258-1259 HYPH denotes -
T20905 1259-1260 NN denotes F
T20907 1260-1262 , denotes ,
T20908 1262-1281 NN denotes ctcctgtctgggctgtgag
T20909 1282-1285 CC denotes and
T20910 1286-1288 NN denotes HG
T20912 1288-1289 HYPH denotes -
T20911 1289-1290 NN denotes R
T20913 1290-1292 , denotes ,
T20914 1292-1312 NN denotes caaaggcagaagtggggtaa
T20915 1312-1313 -RRB- denotes )
T20902 1314-1321 VBD denotes spanned
T20916 1322-1325 DT denotes the
T20918 1326-1328 NN denotes hg
T20917 1329-1337 NN denotes deletion
T20919 1338-1347 VBG denotes producing
T20920 1348-1349 DT denotes a
T20922 1350-1353 CD denotes 447
T20923 1354-1356 NN denotes bp
T20921 1357-1364 NN denotes product
T20924 1365-1367 IN denotes in
T20925 1368-1370 NN denotes hg
T20927 1370-1371 HYPH denotes /
T20926 1371-1373 NN denotes hg
T20929 1374-1377 CC denotes and
T20930 1378-1379 SYM denotes +
T20932 1379-1380 HYPH denotes /
T20931 1380-1382 NN denotes hg
T20928 1383-1387 NNS denotes mice
T20933 1387-1388 . denotes .
T20934 1388-1565 sentence denotes The other set (CRADD3a.F, gtccatcagcattcctgaaa and CRADD3.R, tgtccagcaacagcattgtc) amplified a 232 bp Cradd amplicon (located within the hg deletion) in +/+ and +/hg mice [54].
T20935 1389-1392 DT denotes The
T20937 1393-1398 JJ denotes other
T20936 1399-1402 NN denotes set
T20939 1403-1404 -LRB- denotes (
T20940 1404-1413 NN denotes CRADD3a.F
T20941 1413-1415 , denotes ,
T20942 1415-1435 NN denotes gtccatcagcattcctgaaa
T20943 1436-1439 CC denotes and
T20944 1440-1448 NN denotes CRADD3.R
T20945 1448-1450 , denotes ,
T20946 1450-1470 NN denotes tgtccagcaacagcattgtc
T20947 1470-1471 -RRB- denotes )
T20938 1472-1481 VBD denotes amplified
T20948 1482-1483 DT denotes a
T20950 1484-1487 CD denotes 232
T20951 1488-1490 NN denotes bp
T20952 1491-1496 NN denotes Cradd
T20949 1497-1505 NN denotes amplicon
T20953 1506-1507 -LRB- denotes (
T20954 1507-1514 VBN denotes located
T20955 1515-1521 IN denotes within
T20956 1522-1525 DT denotes the
T20958 1526-1528 NN denotes hg
T20957 1529-1537 NN denotes deletion
T20959 1537-1538 -RRB- denotes )
T20960 1539-1541 IN denotes in
T20961 1542-1543 SYM denotes +
T20963 1543-1544 HYPH denotes /
T20962 1544-1545 SYM denotes +
T20965 1546-1549 CC denotes and
T20966 1550-1551 SYM denotes +
T20968 1551-1552 HYPH denotes /
T20967 1552-1554 NN denotes hg
T20964 1555-1559 NNS denotes mice
T20969 1560-1561 -LRB- denotes [
T20970 1561-1563 CD denotes 54
T20971 1563-1564 -RRB- denotes ]
T20972 1564-1565 . denotes .
T20973 1565-1644 sentence denotes The PCR annealing temperature was 55°C and the MgCl2 concentration was 1.5 mM.
T20974 1566-1569 DT denotes The
T20976 1570-1573 NN denotes PCR
T20977 1574-1583 VBG denotes annealing
T20975 1584-1595 NN denotes temperature
T20978 1596-1599 VBD denotes was
T20979 1600-1602 CD denotes 55
T20980 1602-1604 NN denotes °C
T20981 1605-1608 CC denotes and
T20982 1609-1612 DT denotes the
T20984 1613-1618 NN denotes MgCl2
T20983 1619-1632 NN denotes concentration
T20985 1633-1636 VBD denotes was
T20986 1637-1640 CD denotes 1.5
T20987 1641-1643 NN denotes mM
T20988 1643-1644 . denotes .
T21607 1646-1657 NN denotes Development
T21608 1658-1660 IN denotes of
T21609 1661-1668 NN denotes B6.CAST
T21611 1669-1672 CC denotes and
T21612 1673-1680 NN denotes HG.CAST
T21610 1681-1685 NN denotes MMU2
T21614 1686-1691 NN denotes speed
T21615 1692-1700 JJ denotes congenic
T21613 1701-1708 NNS denotes strains
T21616 1708-1834 sentence denotes All speed congenic strains were developed starting with an initial cross between a CAST male and HG females (Figure 1) [5,6].
T21617 1709-1712 DT denotes All
T21619 1713-1718 NN denotes speed
T21620 1719-1727 JJ denotes congenic
T21618 1728-1735 NNS denotes strains
T21622 1736-1740 VBD denotes were
T21621 1741-1750 VBN denotes developed
T21623 1751-1759 VBG denotes starting
T21624 1760-1764 IN denotes with
T21625 1765-1767 DT denotes an
T21627 1768-1775 JJ denotes initial
T21626 1776-1781 NN denotes cross
T21628 1782-1789 IN denotes between
T21629 1790-1791 DT denotes a
T21631 1792-1796 NN denotes CAST
T21630 1797-1801 NN denotes male
T21632 1802-1805 CC denotes and
T21633 1806-1808 NN denotes HG
T21634 1809-1816 NNS denotes females
T21635 1817-1818 -LRB- denotes (
T21636 1818-1824 NN denotes Figure
T21637 1825-1826 CD denotes 1
T21638 1826-1827 -RRB- denotes )
T21639 1828-1829 -LRB- denotes [
T21641 1829-1830 CD denotes 5
T21642 1830-1831 , denotes ,
T21640 1831-1832 CD denotes 6
T21643 1832-1833 -RRB- denotes ]
T21644 1833-1834 . denotes .
T21645 1834-1884 sentence denotes Male F1 mice were then backcrossed to HG females.
T21646 1835-1839 JJ denotes Male
T21648 1840-1842 NN denotes F1
T21647 1843-1847 NNS denotes mice
T21650 1848-1852 VBD denotes were
T21651 1853-1857 RB denotes then
T21649 1858-1869 VBN denotes backcrossed
T21652 1870-1872 IN denotes to
T21653 1873-1875 NN denotes HG
T21654 1876-1883 NNS denotes females
T21655 1883-1884 . denotes .
T21656 1884-2037 sentence denotes All agouti (the dominant nonagouti (a) locus is located at 154.8 Mbp on MMU2) N2 males were genotyped for 79 microsatellite markers (Additional File 1).
T21657 1885-1888 DT denotes All
T21659 1889-1895 NN denotes agouti
T21660 1896-1897 -LRB- denotes (
T21662 1897-1900 DT denotes the
T21664 1901-1909 JJ denotes dominant
T21665 1910-1919 JJ denotes nonagouti
T21667 1920-1921 -LRB- denotes (
T21666 1921-1922 NN denotes a
T21668 1922-1923 -RRB- denotes )
T21663 1924-1929 NN denotes locus
T21669 1930-1932 VBZ denotes is
T21661 1933-1940 VBN denotes located
T21670 1941-1943 IN denotes at
T21671 1944-1949 CD denotes 154.8
T21672 1950-1953 NN denotes Mbp
T21673 1954-1956 IN denotes on
T21674 1957-1961 NN denotes MMU2
T21675 1961-1962 -RRB- denotes )
T21676 1963-1965 NN denotes N2
T21658 1966-1971 NNS denotes males
T21678 1972-1976 VBD denotes were
T21677 1977-1986 VBN denotes genotyped
T21679 1987-1990 IN denotes for
T21680 1991-1993 CD denotes 79
T21682 1994-2008 NN denotes microsatellite
T21681 2009-2016 NNS denotes markers
T21683 2017-2018 -LRB- denotes (
T21685 2018-2028 JJ denotes Additional
T21684 2029-2033 NN denotes File
T21686 2034-2035 CD denotes 1
T21687 2035-2036 -RRB- denotes )
T21688 2036-2037 . denotes .
T21689 2037-2185 sentence denotes These markers were evenly spaced across the genome, except in regions previously identified as harboring QTL [1], which were more densely screened.
T21690 2038-2043 DT denotes These
T21691 2044-2051 NNS denotes markers
T21693 2052-2056 VBD denotes were
T21694 2057-2063 RB denotes evenly
T21692 2064-2070 VBN denotes spaced
T21695 2071-2077 IN denotes across
T21696 2078-2081 DT denotes the
T21697 2082-2088 NN denotes genome
T21698 2088-2090 , denotes ,
T21699 2090-2096 RB denotes except
T21700 2097-2099 IN denotes in
T21701 2100-2107 NNS denotes regions
T21702 2108-2118 RB denotes previously
T21703 2119-2129 VBN denotes identified
T21704 2130-2132 IN denotes as
T21705 2133-2142 VBG denotes harboring
T21706 2143-2146 NN denotes QTL
T21707 2147-2148 -LRB- denotes [
T21708 2148-2149 CD denotes 1
T21709 2149-2150 -RRB- denotes ]
T21710 2150-2152 , denotes ,
T21711 2152-2157 WDT denotes which
T21713 2158-2162 VBD denotes were
T21714 2163-2167 RBR denotes more
T21715 2168-2175 RB denotes densely
T21712 2176-2184 VBN denotes screened
T21716 2184-2185 . denotes .
T21717 2185-2351 sentence denotes The "best" N2 agouti male with the lowest level of genome-wide unwanted heterozygosity while maintaining CAST alleles for all MMU2 markers was selected for breeding.
T21718 2186-2189 DT denotes The
T21720 2190-2191 `` denotes "
T21721 2191-2195 JJS denotes best
T21722 2195-2196 '' denotes "
T21723 2197-2199 NN denotes N2
T21724 2200-2206 NN denotes agouti
T21719 2207-2211 NN denotes male
T21726 2212-2216 IN denotes with
T21727 2217-2220 DT denotes the
T21729 2221-2227 JJS denotes lowest
T21728 2228-2233 NN denotes level
T21730 2234-2236 IN denotes of
T21731 2237-2243 NN denotes genome
T21733 2243-2244 HYPH denotes -
T21732 2244-2248 JJ denotes wide
T21735 2249-2257 JJ denotes unwanted
T21734 2258-2272 NN denotes heterozygosity
T21736 2273-2278 IN denotes while
T21737 2279-2290 VBG denotes maintaining
T21738 2291-2295 NN denotes CAST
T21739 2296-2303 NNS denotes alleles
T21740 2304-2307 IN denotes for
T21741 2308-2311 DT denotes all
T21743 2312-2316 NN denotes MMU2
T21742 2317-2324 NNS denotes markers
T21744 2325-2328 VBD denotes was
T21725 2329-2337 VBN denotes selected
T21745 2338-2341 IN denotes for
T21746 2342-2350 NN denotes breeding
T21747 2350-2351 . denotes .
T21748 2351-2485 sentence denotes This selection scheme was used at each generation until a N4 male was identified as homozygous HG for all markers typed outside MMU2.
T21749 2352-2356 DT denotes This
T21751 2357-2366 NN denotes selection
T21750 2367-2373 NN denotes scheme
T21753 2374-2377 VBD denotes was
T21752 2378-2382 VBN denotes used
T21754 2383-2385 IN denotes at
T21755 2386-2390 DT denotes each
T21756 2391-2401 NN denotes generation
T21757 2402-2407 IN denotes until
T21759 2408-2409 DT denotes a
T21761 2410-2412 NN denotes N4
T21760 2413-2417 NN denotes male
T21762 2418-2421 VBD denotes was
T21758 2422-2432 VBN denotes identified
T21763 2433-2435 IN denotes as
T21764 2436-2446 JJ denotes homozygous
T21765 2447-2449 NN denotes HG
T21766 2450-2453 IN denotes for
T21767 2454-2457 DT denotes all
T21768 2458-2465 NNS denotes markers
T21769 2466-2471 VBN denotes typed
T21770 2472-2479 IN denotes outside
T21771 2480-2484 NN denotes MMU2
T21772 2484-2485 . denotes .
T21773 2485-2629 sentence denotes After an additional backcross to HG females, recombinant males were identified providing the foundation for the four overlapping donor regions.
T21774 2486-2491 IN denotes After
T21776 2492-2494 DT denotes an
T21778 2495-2505 JJ denotes additional
T21777 2506-2515 NN denotes backcross
T21779 2516-2518 IN denotes to
T21780 2519-2521 NN denotes HG
T21781 2522-2529 NNS denotes females
T21782 2529-2531 , denotes ,
T21783 2531-2542 JJ denotes recombinant
T21784 2543-2548 NNS denotes males
T21785 2549-2553 VBD denotes were
T21775 2554-2564 VBN denotes identified
T21786 2565-2574 VBG denotes providing
T21787 2575-2578 DT denotes the
T21788 2579-2589 NN denotes foundation
T21789 2590-2593 IN denotes for
T21790 2594-2597 DT denotes the
T21792 2598-2602 CD denotes four
T21793 2603-2614 VBG denotes overlapping
T21794 2615-2620 NN denotes donor
T21791 2621-2628 NNS denotes regions
T21795 2628-2629 . denotes .
T21796 2629-2784 sentence denotes Selected recombinant males were then backcrossed to both B6 and HG females to create strains, which were B6 (+/+) or HG (hg/hg) and heterozygous congenic.
T21797 2630-2638 VBN denotes Selected
T21799 2639-2650 JJ denotes recombinant
T21798 2651-2656 NNS denotes males
T21801 2657-2661 VBD denotes were
T21802 2662-2666 RB denotes then
T21800 2667-2678 VBN denotes backcrossed
T21803 2679-2681 IN denotes to
T21804 2682-2686 CC denotes both
T21805 2687-2689 NN denotes B6
T21807 2690-2693 CC denotes and
T21808 2694-2696 NN denotes HG
T21806 2697-2704 NNS denotes females
T21809 2705-2707 TO denotes to
T21810 2708-2714 VB denotes create
T21811 2715-2722 NNS denotes strains
T21812 2722-2724 , denotes ,
T21813 2724-2729 WDT denotes which
T21814 2730-2734 VBD denotes were
T21815 2735-2737 NN denotes B6
T21817 2738-2739 -LRB- denotes (
T21819 2739-2740 SYM denotes +
T21820 2740-2741 HYPH denotes /
T21818 2741-2742 SYM denotes +
T21821 2742-2743 -RRB- denotes )
T21822 2744-2746 CC denotes or
T21823 2747-2749 NN denotes HG
T21824 2750-2751 -LRB- denotes (
T21826 2751-2753 NN denotes hg
T21827 2753-2754 HYPH denotes /
T21825 2754-2756 NN denotes hg
T21828 2756-2757 -RRB- denotes )
T21829 2758-2761 CC denotes and
T21830 2762-2774 JJ denotes heterozygous
T21816 2775-2783 JJ denotes congenic
T21831 2783-2784 . denotes .
T21832 2784-2859 sentence denotes These mice were intermated to produce homozygous founders for each strain.
T21833 2785-2790 DT denotes These
T21834 2791-2795 NNS denotes mice
T21836 2796-2800 VBD denotes were
T21835 2801-2811 VBN denotes intermated
T21837 2812-2814 TO denotes to
T21838 2815-2822 VB denotes produce
T21839 2823-2833 JJ denotes homozygous
T21840 2834-2842 NNS denotes founders
T21841 2843-2846 IN denotes for
T21842 2847-2851 DT denotes each
T21843 2852-2858 NN denotes strain
T21844 2858-2859 . denotes .
T21845 2859-3073 sentence denotes This novel breeding scheme created four identical founder congenics on two backgrounds B6 (+/+) and HG (hg/hg), which formed the basis for our examination of interactions caused by the presence of the hg deletion.
T21846 2860-2864 DT denotes This
T21848 2865-2870 JJ denotes novel
T21849 2871-2879 VBG denotes breeding
T21847 2880-2886 NN denotes scheme
T21850 2887-2894 VBD denotes created
T21851 2895-2899 CD denotes four
T21853 2900-2909 JJ denotes identical
T21854 2910-2917 NN denotes founder
T21852 2918-2927 NNS denotes congenics
T21855 2928-2930 IN denotes on
T21856 2931-2934 CD denotes two
T21857 2935-2946 NNS denotes backgrounds
T21858 2947-2949 NN denotes B6
T21859 2950-2951 -LRB- denotes (
T21861 2951-2952 SYM denotes +
T21862 2952-2953 HYPH denotes /
T21860 2953-2954 SYM denotes +
T21863 2954-2955 -RRB- denotes )
T21864 2956-2959 CC denotes and
T21865 2960-2962 NN denotes HG
T21866 2963-2964 -LRB- denotes (
T21868 2964-2966 NN denotes hg
T21869 2966-2967 HYPH denotes /
T21867 2967-2969 NN denotes hg
T21870 2969-2970 -RRB- denotes )
T21871 2970-2972 , denotes ,
T21872 2972-2977 WDT denotes which
T21873 2978-2984 VBD denotes formed
T21874 2985-2988 DT denotes the
T21875 2989-2994 NN denotes basis
T21876 2995-2998 IN denotes for
T21877 2999-3002 PRP$ denotes our
T21878 3003-3014 NN denotes examination
T21879 3015-3017 IN denotes of
T21880 3018-3030 NNS denotes interactions
T21881 3031-3037 VBN denotes caused
T21882 3038-3040 IN denotes by
T21883 3041-3044 DT denotes the
T21884 3045-3053 NN denotes presence
T21885 3054-3056 IN denotes of
T21886 3057-3060 DT denotes the
T21888 3061-3063 NN denotes hg
T21887 3064-3072 NN denotes deletion
T21889 3072-3073 . denotes .
T21890 3073-3148 sentence denotes MMU2 speed congenic strains were maintained through brother-sister mating.
T21891 3074-3078 NN denotes MMU2
T21893 3079-3084 NN denotes speed
T21894 3085-3093 JJ denotes congenic
T21892 3094-3101 NNS denotes strains
T21896 3102-3106 VBD denotes were
T21895 3107-3117 VBN denotes maintained
T21897 3118-3125 IN denotes through
T21898 3126-3133 NN denotes brother
T21900 3133-3134 HYPH denotes -
T21899 3134-3140 NN denotes sister
T21901 3141-3147 NN denotes mating
T21902 3147-3148 . denotes .
T21903 3148-3313 sentence denotes Once each congenic was stabilized, 20 additional microsatellite markers were used to refine the position of each congenic recombinant end point (Additional File 2).
T21904 3149-3153 RB denotes Once
T21906 3154-3158 DT denotes each
T21907 3159-3167 JJ denotes congenic
T21908 3168-3171 VBD denotes was
T21905 3172-3182 VBN denotes stabilized
T21910 3182-3184 , denotes ,
T21911 3184-3186 CD denotes 20
T21913 3187-3197 JJ denotes additional
T21914 3198-3212 NN denotes microsatellite
T21912 3213-3220 NNS denotes markers
T21915 3221-3225 VBD denotes were
T21909 3226-3230 VBN denotes used
T21916 3231-3233 TO denotes to
T21917 3234-3240 VB denotes refine
T21918 3241-3244 DT denotes the
T21919 3245-3253 NN denotes position
T21920 3254-3256 IN denotes of
T21921 3257-3261 DT denotes each
T21923 3262-3270 JJ denotes congenic
T21924 3271-3282 JJ denotes recombinant
T21925 3283-3286 NN denotes end
T21922 3287-3292 NN denotes point
T21926 3293-3294 -LRB- denotes (
T21928 3294-3304 JJ denotes Additional
T21927 3305-3309 NN denotes File
T21929 3310-3311 CD denotes 2
T21930 3311-3312 -RRB- denotes )
T21931 3312-3313 . denotes .
T22598 3315-3326 NN denotes Development
T22599 3327-3329 IN denotes of
T22600 3330-3337 NN denotes HG.CAST
T22602 3338-3343 NN denotes speed
T22603 3344-3352 JJ denotes congenic
T22601 3353-3360 NNS denotes strains
T22604 3361-3364 IN denotes for
T22605 3365-3368 NN denotes MMU
T22606 3368-3369 CD denotes 1
T22607 3369-3371 , denotes ,
T22608 3371-3372 CD denotes 5
T22609 3372-3374 , denotes ,
T22610 3374-3375 CD denotes 8
T22611 3375-3377 , denotes ,
T22612 3377-3378 CD denotes 9
T22613 3378-3380 , denotes ,
T22614 3380-3382 CD denotes 11
T22615 3383-3386 CC denotes and
T22616 3387-3389 CD denotes 17
T22617 3389-3692 sentence denotes All black N2 males from the first two crosses described above were genotyped for 12 markers (D1Mit432, -480, D5Mit353, -311, D9Mit60, -262, D11Mit5, -67, D8Mit234, -211 and D17Mit28 and -142), two spanning each of the six QTL harboring regions (MMU1, 5, 8, 9, 11 and 17; Table 1 and Additional File 1).
T22618 3390-3393 DT denotes All
T22620 3394-3399 JJ denotes black
T22621 3400-3402 NN denotes N2
T22619 3403-3408 NNS denotes males
T22623 3409-3413 IN denotes from
T22624 3414-3417 DT denotes the
T22626 3418-3423 JJ denotes first
T22627 3424-3427 CD denotes two
T22625 3428-3435 NNS denotes crosses
T22628 3436-3445 VBN denotes described
T22629 3446-3451 RB denotes above
T22630 3452-3456 VBD denotes were
T22622 3457-3466 VBN denotes genotyped
T22631 3467-3470 IN denotes for
T22632 3471-3473 CD denotes 12
T22633 3474-3481 NNS denotes markers
T22634 3482-3483 -LRB- denotes (
T22635 3483-3491 NN denotes D1Mit432
T22636 3491-3493 , denotes ,
T22637 3493-3494 SYM denotes -
T22638 3494-3497 CD denotes 480
T22639 3497-3499 , denotes ,
T22640 3499-3507 NN denotes D5Mit353
T22641 3507-3509 , denotes ,
T22642 3509-3510 SYM denotes -
T22643 3510-3513 CD denotes 311
T22644 3513-3515 , denotes ,
T22645 3515-3522 NN denotes D9Mit60
T22646 3522-3524 , denotes ,
T22647 3524-3525 SYM denotes -
T22648 3525-3528 CD denotes 262
T22649 3528-3530 , denotes ,
T22650 3530-3537 NN denotes D11Mit5
T22651 3537-3539 , denotes ,
T22652 3539-3540 SYM denotes -
T22653 3540-3542 CD denotes 67
T22654 3542-3544 , denotes ,
T22655 3544-3552 NN denotes D8Mit234
T22656 3552-3554 , denotes ,
T22657 3554-3555 SYM denotes -
T22658 3555-3558 CD denotes 211
T22659 3559-3562 CC denotes and
T22660 3563-3571 NN denotes D17Mit28
T22661 3572-3575 CC denotes and
T22662 3576-3577 SYM denotes -
T22663 3577-3580 CD denotes 142
T22664 3580-3581 -RRB- denotes )
T22665 3581-3583 , denotes ,
T22666 3583-3586 CD denotes two
T22667 3587-3595 VBG denotes spanning
T22668 3596-3600 DT denotes each
T22669 3601-3603 IN denotes of
T22670 3604-3607 DT denotes the
T22672 3608-3611 CD denotes six
T22673 3612-3615 NNS denotes QTL
T22674 3616-3625 VBG denotes harboring
T22671 3626-3633 NNS denotes regions
T22675 3634-3635 -LRB- denotes (
T22676 3635-3638 NN denotes MMU
T22677 3638-3639 CD denotes 1
T22678 3639-3641 , denotes ,
T22679 3641-3642 CD denotes 5
T22680 3642-3644 , denotes ,
T22681 3644-3645 CD denotes 8
T22682 3645-3647 , denotes ,
T22683 3647-3648 CD denotes 9
T22684 3648-3650 , denotes ,
T22685 3650-3652 CD denotes 11
T22686 3653-3656 CC denotes and
T22687 3657-3659 CD denotes 17
T22688 3659-3660 : denotes ;
T22689 3661-3666 NN denotes Table
T22690 3667-3668 CD denotes 1
T22691 3669-3672 CC denotes and
T22692 3673-3683 JJ denotes Additional
T22693 3684-3688 NN denotes File
T22694 3689-3690 CD denotes 1
T22695 3690-3691 -RRB- denotes )
T22696 3691-3692 . denotes .
T22697 3692-3768 sentence denotes Markers were selected to capture, at a minimum, the 2-LOD support interval.
T22698 3693-3700 NNS denotes Markers
T22700 3701-3705 VBD denotes were
T22699 3706-3714 VBN denotes selected
T22701 3715-3717 TO denotes to
T22702 3718-3725 VB denotes capture
T22703 3725-3727 , denotes ,
T22704 3727-3729 IN denotes at
T22705 3730-3731 DT denotes a
T22706 3732-3739 NN denotes minimum
T22707 3739-3741 , denotes ,
T22708 3741-3744 DT denotes the
T22710 3745-3746 CD denotes 2
T22712 3746-3747 HYPH denotes -
T22711 3747-3750 NN denotes LOD
T22713 3751-3758 NN denotes support
T22709 3759-3767 NN denotes interval
T22714 3767-3768 . denotes .
T22715 3768-3935 sentence denotes Two N2 males were selected to propagate the N3 generation; one heterozygous for QTL on MMU1 and 9 and the other heterozygous for QTL on MMU5, 8, 11 and 17 (Figure 1).
T22716 3769-3772 CD denotes Two
T22718 3773-3775 NN denotes N2
T22717 3776-3781 NNS denotes males
T22720 3782-3786 VBD denotes were
T22719 3787-3795 VBN denotes selected
T22721 3796-3798 TO denotes to
T22722 3799-3808 VB denotes propagate
T22723 3809-3812 DT denotes the
T22725 3813-3815 NN denotes N3
T22724 3816-3826 NN denotes generation
T22726 3826-3827 : denotes ;
T22727 3828-3831 CD denotes one
T22728 3832-3844 JJ denotes heterozygous
T22729 3845-3848 IN denotes for
T22730 3849-3852 NN denotes QTL
T22731 3853-3855 IN denotes on
T22732 3856-3859 NN denotes MMU
T22733 3859-3860 CD denotes 1
T22734 3861-3864 CC denotes and
T22735 3865-3866 CD denotes 9
T22736 3867-3870 CC denotes and
T22737 3871-3874 DT denotes the
T22738 3875-3880 JJ denotes other
T22739 3881-3893 NN denotes heterozygous
T22740 3894-3897 IN denotes for
T22741 3898-3901 NN denotes QTL
T22742 3902-3904 IN denotes on
T22743 3905-3908 NN denotes MMU
T22744 3908-3909 CD denotes 5
T22745 3909-3911 , denotes ,
T22746 3911-3912 CD denotes 8
T22747 3912-3914 , denotes ,
T22748 3914-3916 CD denotes 11
T22749 3917-3920 CC denotes and
T22750 3921-3923 CD denotes 17
T22751 3924-3925 -LRB- denotes (
T22752 3925-3931 NN denotes Figure
T22753 3932-3933 CD denotes 1
T22754 3933-3934 -RRB- denotes )
T22755 3934-3935 . denotes .
T22756 3935-4001 sentence denotes Both males were homozygous for HG alleles at all other known QTL.
T22757 3936-3940 DT denotes Both
T22758 3941-3946 NNS denotes males
T22759 3947-3951 VBD denotes were
T22760 3952-3962 JJ denotes homozygous
T22761 3963-3966 IN denotes for
T22762 3967-3969 NN denotes HG
T22763 3970-3977 NNS denotes alleles
T22764 3978-3980 IN denotes at
T22765 3981-3984 DT denotes all
T22767 3985-3990 JJ denotes other
T22768 3991-3996 VBN denotes known
T22766 3997-4000 NN denotes QTL
T22769 4000-4001 . denotes .
T22770 4001-4152 sentence denotes These males were backcrossed to HG females and two of the resulting N3 males inheriting the same sets of QTL as their sire were selected for breeding.
T22771 4002-4007 DT denotes These
T22772 4008-4013 NNS denotes males
T22774 4014-4018 VBD denotes were
T22773 4019-4030 VBN denotes backcrossed
T22775 4031-4033 IN denotes to
T22776 4034-4036 NN denotes HG
T22777 4037-4044 NNS denotes females
T22778 4045-4048 CC denotes and
T22779 4049-4052 CD denotes two
T22781 4053-4055 IN denotes of
T22782 4056-4059 DT denotes the
T22784 4060-4069 VBG denotes resulting
T22785 4070-4072 NN denotes N3
T22783 4073-4078 NNS denotes males
T22786 4079-4089 VBG denotes inheriting
T22787 4090-4093 DT denotes the
T22789 4094-4098 JJ denotes same
T22788 4099-4103 NNS denotes sets
T22790 4104-4106 IN denotes of
T22791 4107-4110 NN denotes QTL
T22792 4111-4113 IN denotes as
T22793 4114-4119 PRP$ denotes their
T22794 4120-4124 NN denotes sire
T22795 4125-4129 VBD denotes were
T22780 4130-4138 VBN denotes selected
T22796 4139-4142 IN denotes for
T22797 4143-4151 NN denotes breeding
T22798 4151-4152 . denotes .
T22799 4152-4345 sentence denotes These males were subsequently backcrossed to HG females and three N4 males were identified heterozygous for the following regions: 1) MMU1 and 9; 2) MMU5 and 11; 3) MMU8, 11 and 17 (Figure 1).
T22800 4153-4158 DT denotes These
T22801 4159-4164 NNS denotes males
T22803 4165-4169 VBD denotes were
T22804 4170-4182 RB denotes subsequently
T22802 4183-4194 VBN denotes backcrossed
T22805 4195-4197 IN denotes to
T22806 4198-4200 NN denotes HG
T22807 4201-4208 NNS denotes females
T22808 4209-4212 CC denotes and
T22809 4213-4218 CD denotes three
T22811 4219-4221 NN denotes N4
T22810 4222-4227 NNS denotes males
T22813 4228-4232 VBD denotes were
T22812 4233-4243 VBN denotes identified
T22814 4244-4256 JJ denotes heterozygous
T22815 4257-4260 IN denotes for
T22816 4261-4264 DT denotes the
T22818 4265-4274 VBG denotes following
T22817 4275-4282 NNS denotes regions
T22819 4282-4284 : denotes :
T22820 4284-4285 LS denotes 1
T22822 4285-4286 -RRB- denotes )
T22823 4287-4290 NN denotes MMU
T22821 4290-4291 CD denotes 1
T22824 4292-4295 CC denotes and
T22825 4296-4297 CD denotes 9
T22826 4297-4298 : denotes ;
T22827 4299-4300 LS denotes 2
T22829 4300-4301 -RRB- denotes )
T22830 4302-4305 NN denotes MMU
T22828 4305-4306 CD denotes 5
T22831 4307-4310 CC denotes and
T22832 4311-4313 CD denotes 11
T22833 4313-4314 : denotes ;
T22834 4315-4316 LS denotes 3
T22836 4316-4317 -RRB- denotes )
T22837 4318-4321 NN denotes MMU
T22835 4321-4322 CD denotes 8
T22838 4322-4324 , denotes ,
T22839 4324-4326 CD denotes 11
T22840 4327-4330 CC denotes and
T22841 4331-4333 CD denotes 17
T22842 4334-4335 -LRB- denotes (
T22843 4335-4341 NN denotes Figure
T22844 4342-4343 CD denotes 1
T22845 4343-4344 -RRB- denotes )
T22846 4344-4345 . denotes .
T22847 4345-4652 sentence denotes Starting at N4 and continuing through N6, the "best" male with the lowest percent of unwanted donor alleles was selected after performing a genome scan using the remaining 67 genome-wide markers (79 total markers minus the 12 markers genotyped in the first two backcrosses spanning the know QTL intervals).
T22848 4346-4354 VBG denotes Starting
T22850 4355-4357 IN denotes at
T22851 4358-4360 NN denotes N4
T22852 4361-4364 CC denotes and
T22853 4365-4375 VBG denotes continuing
T22854 4376-4383 IN denotes through
T22855 4384-4386 NN denotes N6
T22856 4386-4388 , denotes ,
T22857 4388-4391 DT denotes the
T22859 4392-4393 `` denotes "
T22860 4393-4397 JJS denotes best
T22861 4397-4398 '' denotes "
T22858 4399-4403 JJ denotes male
T22862 4404-4408 IN denotes with
T22863 4409-4412 DT denotes the
T22865 4413-4419 JJS denotes lowest
T22864 4420-4427 NN denotes percent
T22866 4428-4430 IN denotes of
T22867 4431-4439 JJ denotes unwanted
T22869 4440-4445 NN denotes donor
T22868 4446-4453 NNS denotes alleles
T22870 4454-4457 VBD denotes was
T22849 4458-4466 VBN denotes selected
T22871 4467-4472 IN denotes after
T22872 4473-4483 VBG denotes performing
T22873 4484-4485 DT denotes a
T22875 4486-4492 NN denotes genome
T22874 4493-4497 NN denotes scan
T22876 4498-4503 VBG denotes using
T22877 4504-4507 DT denotes the
T22879 4508-4517 VBG denotes remaining
T22880 4518-4520 CD denotes 67
T22881 4521-4527 NN denotes genome
T22883 4527-4528 HYPH denotes -
T22882 4528-4532 JJ denotes wide
T22878 4533-4540 NNS denotes markers
T22884 4541-4542 -LRB- denotes (
T22886 4542-4544 CD denotes 79
T22887 4545-4550 JJ denotes total
T22885 4551-4558 NNS denotes markers
T22888 4559-4564 CC denotes minus
T22889 4565-4568 DT denotes the
T22891 4569-4571 CD denotes 12
T22890 4572-4579 NNS denotes markers
T22892 4580-4589 VBN denotes genotyped
T22893 4590-4592 IN denotes in
T22894 4593-4596 DT denotes the
T22896 4597-4602 JJ denotes first
T22897 4603-4606 CD denotes two
T22895 4607-4618 NNS denotes backcrosses
T22898 4619-4627 VBG denotes spanning
T22899 4628-4631 DT denotes the
T22901 4632-4636 JJ denotes know
T22902 4637-4640 NN denotes QTL
T22900 4641-4650 NNS denotes intervals
T22903 4650-4651 -RRB- denotes )
T22904 4651-4652 . denotes .
T22905 4652-4783 sentence denotes At N5 a distinct strain was created for each of the six individual donor regions and heterozygous mice were intermated (Figure 1).
T22906 4653-4655 IN denotes At
T22908 4656-4658 NN denotes N5
T22909 4659-4660 DT denotes a
T22911 4661-4669 JJ denotes distinct
T22910 4670-4676 NN denotes strain
T22912 4677-4680 VBD denotes was
T22907 4681-4688 VBN denotes created
T22913 4689-4692 IN denotes for
T22914 4693-4697 DT denotes each
T22915 4698-4700 IN denotes of
T22916 4701-4704 DT denotes the
T22918 4705-4708 CD denotes six
T22919 4709-4719 JJ denotes individual
T22920 4720-4725 NN denotes donor
T22917 4726-4733 NNS denotes regions
T22921 4734-4737 CC denotes and
T22922 4738-4750 JJ denotes heterozygous
T22923 4751-4755 NNS denotes mice
T22925 4756-4760 VBD denotes were
T22924 4761-4771 VBN denotes intermated
T22926 4772-4773 -LRB- denotes (
T22927 4773-4779 NN denotes Figure
T22928 4780-4781 CD denotes 1
T22929 4781-4782 -RRB- denotes )
T22930 4782-4783 . denotes .
T22931 4783-4872 sentence denotes Homozygous HG.CAST speed congenic strains were maintained through brother-sister mating.
T22932 4784-4794 JJ denotes Homozygous
T22934 4795-4802 NN denotes HG.CAST
T22935 4803-4808 NN denotes speed
T22936 4809-4817 JJ denotes congenic
T22933 4818-4825 NNS denotes strains
T22938 4826-4830 VBD denotes were
T22937 4831-4841 VBN denotes maintained
T22939 4842-4849 IN denotes through
T22940 4850-4857 NN denotes brother
T22942 4857-4858 HYPH denotes -
T22941 4858-4864 NN denotes sister
T22943 4865-4871 NN denotes mating
T22944 4871-4872 . denotes .
T22945 4872-5037 sentence denotes Once each congenic was stabilized, 19 additional microsatellite markers were used to refine the position of each congenic recombinant end point (Additional File 2).
T22946 4873-4877 IN denotes Once
T22948 4878-4882 DT denotes each
T22949 4883-4891 JJ denotes congenic
T22950 4892-4895 VBD denotes was
T22947 4896-4906 VBN denotes stabilized
T22952 4906-4908 , denotes ,
T22953 4908-4910 CD denotes 19
T22955 4911-4921 JJ denotes additional
T22956 4922-4936 NN denotes microsatellite
T22954 4937-4944 NNS denotes markers
T22957 4945-4949 VBD denotes were
T22951 4950-4954 VBN denotes used
T22958 4955-4957 TO denotes to
T22959 4958-4964 VB denotes refine
T22960 4965-4968 DT denotes the
T22961 4969-4977 NN denotes position
T22962 4978-4980 IN denotes of
T22963 4981-4985 DT denotes each
T22965 4986-4994 JJ denotes congenic
T22966 4995-5006 JJ denotes recombinant
T22967 5007-5010 NN denotes end
T22964 5011-5016 NN denotes point
T22968 5017-5018 -LRB- denotes (
T22970 5018-5028 JJ denotes Additional
T22969 5029-5033 NN denotes File
T22971 5034-5035 CD denotes 2
T22972 5035-5036 -RRB- denotes )
T22973 5036-5037 . denotes .
T23420 5039-5050 NN denotes Development
T23421 5051-5053 IN denotes of
T23422 5054-5062 NN denotes B6.CASTC
T23424 5063-5066 CC denotes and
T23425 5067-5075 NN denotes HG.CASTC
T23426 5076-5083 NN denotes control
T23423 5084-5091 NNS denotes strains
T23427 5091-5414 sentence denotes HG is a strain in which the hg deletion has been introgressed onto a B6 background, therefore the only genetic differences between the strains would be the hg locus, tightly linked alleles from the outbred strain on which hg arose and contaminating alleles remaining after the nine backcrosses and fixed during inbreeding.
T23428 5092-5094 NN denotes HG
T23429 5095-5097 VBZ denotes is
T23431 5098-5099 DT denotes a
T23432 5100-5106 NN denotes strain
T23433 5107-5109 IN denotes in
T23435 5110-5115 WDT denotes which
T23436 5116-5119 DT denotes the
T23438 5120-5122 NN denotes hg
T23437 5123-5131 NN denotes deletion
T23439 5132-5135 VBZ denotes has
T23440 5136-5140 VBN denotes been
T23434 5141-5153 VBN denotes introgressed
T23441 5154-5158 IN denotes onto
T23442 5159-5160 DT denotes a
T23444 5161-5163 NN denotes B6
T23443 5164-5174 NN denotes background
T23445 5174-5176 , denotes ,
T23446 5176-5185 RB denotes therefore
T23447 5186-5189 DT denotes the
T23449 5190-5194 JJ denotes only
T23450 5195-5202 JJ denotes genetic
T23448 5203-5214 NNS denotes differences
T23451 5215-5222 IN denotes between
T23452 5223-5226 DT denotes the
T23453 5227-5234 NNS denotes strains
T23454 5235-5240 MD denotes would
T23430 5241-5243 VB denotes be
T23455 5244-5247 DT denotes the
T23457 5248-5250 NN denotes hg
T23456 5251-5256 NN denotes locus
T23458 5256-5258 , denotes ,
T23459 5258-5265 RB denotes tightly
T23460 5266-5272 VBN denotes linked
T23461 5273-5280 NNS denotes alleles
T23462 5281-5285 IN denotes from
T23463 5286-5289 DT denotes the
T23465 5290-5297 VBN denotes outbred
T23464 5298-5304 NN denotes strain
T23466 5305-5307 IN denotes on
T23468 5308-5313 WDT denotes which
T23469 5314-5316 NN denotes hg
T23467 5317-5322 VBD denotes arose
T23470 5323-5326 CC denotes and
T23471 5327-5340 VBG denotes contaminating
T23472 5341-5348 NNS denotes alleles
T23473 5349-5358 VBG denotes remaining
T23474 5359-5364 IN denotes after
T23475 5365-5368 DT denotes the
T23477 5369-5373 CD denotes nine
T23476 5374-5385 NNS denotes backcrosses
T23478 5386-5389 CC denotes and
T23479 5390-5395 VBN denotes fixed
T23480 5396-5402 IN denotes during
T23481 5403-5413 NN denotes inbreeding
T23482 5413-5414 . denotes .
T23483 5414-5628 sentence denotes Instead of using parental B6 and HG strains as controls for phenotypic comparisons with each speed congenic, we choose to develop independent control strains originating from the same cross as the congenic panels.
T23484 5415-5422 RB denotes Instead
T23485 5423-5425 IN denotes of
T23487 5426-5431 VBG denotes using
T23488 5432-5440 JJ denotes parental
T23490 5441-5443 NN denotes B6
T23491 5444-5447 CC denotes and
T23492 5448-5450 NN denotes HG
T23489 5451-5458 NNS denotes strains
T23493 5459-5461 IN denotes as
T23494 5462-5470 NNS denotes controls
T23495 5471-5474 IN denotes for
T23496 5475-5485 JJ denotes phenotypic
T23497 5486-5497 NNS denotes comparisons
T23498 5498-5502 IN denotes with
T23499 5503-5507 DT denotes each
T23500 5508-5513 NN denotes speed
T23501 5514-5522 JJ denotes congenic
T23502 5522-5524 , denotes ,
T23503 5524-5526 PRP denotes we
T23486 5527-5533 VBP denotes choose
T23504 5534-5536 TO denotes to
T23505 5537-5544 VB denotes develop
T23506 5545-5556 JJ denotes independent
T23508 5557-5564 NN denotes control
T23507 5565-5572 NNS denotes strains
T23509 5573-5584 VBG denotes originating
T23510 5585-5589 IN denotes from
T23511 5590-5593 DT denotes the
T23513 5594-5598 JJ denotes same
T23512 5599-5604 NN denotes cross
T23514 5605-5607 IN denotes as
T23515 5608-5611 DT denotes the
T23517 5612-5620 JJ denotes congenic
T23516 5621-5627 NNS denotes panels
T23518 5627-5628 . denotes .
T23519 5628-5745 sentence denotes Separate B6.CAST control (B6C) and HG.CAST control (HGC) strains were developed using mice from the MMU2 experiment.
T23520 5629-5637 JJ denotes Separate
T23522 5638-5645 NN denotes B6.CAST
T23523 5646-5653 NN denotes control
T23524 5654-5655 -LRB- denotes (
T23525 5655-5658 NN denotes B6C
T23526 5658-5659 -RRB- denotes )
T23527 5660-5663 CC denotes and
T23528 5664-5671 NN denotes HG.CAST
T23529 5672-5679 NN denotes control
T23530 5680-5681 -LRB- denotes (
T23531 5681-5684 NN denotes HGC
T23532 5684-5685 -RRB- denotes )
T23521 5686-5693 NNS denotes strains
T23534 5694-5698 VBD denotes were
T23533 5699-5708 VBN denotes developed
T23535 5709-5714 VBG denotes using
T23536 5715-5719 NNS denotes mice
T23537 5720-5724 IN denotes from
T23538 5725-5728 DT denotes the
T23540 5729-5733 NN denotes MMU2
T23539 5734-5744 NN denotes experiment
T23541 5744-5745 . denotes .
T23542 5745-5893 sentence denotes Mice from the last backcross inheriting only B6 or HG MMU2 alleles at markers spanning MMU2 were intermated to serve as the basis for each control.
T23543 5746-5750 NNS denotes Mice
T23545 5751-5755 IN denotes from
T23546 5756-5759 DT denotes the
T23548 5760-5764 JJ denotes last
T23547 5765-5774 NN denotes backcross
T23549 5775-5785 VBG denotes inheriting
T23550 5786-5790 RB denotes only
T23552 5791-5793 NN denotes B6
T23553 5794-5796 CC denotes or
T23554 5797-5799 NN denotes HG
T23555 5800-5804 NN denotes MMU2
T23551 5805-5812 NNS denotes alleles
T23556 5813-5815 IN denotes at
T23557 5816-5823 NNS denotes markers
T23558 5824-5832 VBG denotes spanning
T23559 5833-5837 NN denotes MMU2
T23560 5838-5842 VBD denotes were
T23544 5843-5853 VBN denotes intermated
T23561 5854-5856 TO denotes to
T23562 5857-5862 VB denotes serve
T23563 5863-5865 IN denotes as
T23564 5866-5869 DT denotes the
T23565 5870-5875 NN denotes basis
T23566 5876-5879 IN denotes for
T23567 5880-5884 DT denotes each
T23568 5885-5892 NN denotes control
T23569 5892-5893 . denotes .
T23570 5893-5974 sentence denotes Both control strains were subsequently maintained through brother-sister mating.
T23571 5894-5898 DT denotes Both
T23573 5899-5906 NN denotes control
T23572 5907-5914 NNS denotes strains
T23575 5915-5919 VBD denotes were
T23576 5920-5932 RB denotes subsequently
T23574 5933-5943 VBN denotes maintained
T23577 5944-5951 IN denotes through
T23578 5952-5959 NN denotes brother
T23580 5959-5960 HYPH denotes -
T23579 5960-5966 NN denotes sister
T23581 5967-5973 NN denotes mating
T23582 5973-5974 . denotes .
T23583 5974-6198 sentence denotes The control strains were coisogenic with the parental B6 or HG strain with the exception of mutations that arose during congenic construction and a small percentage of contaminating donor alleles missed after 6 backcrosses.
T23584 5975-5978 DT denotes The
T23586 5979-5986 NN denotes control
T23585 5987-5994 NNS denotes strains
T23587 5995-5999 VBD denotes were
T23588 6000-6010 JJ denotes coisogenic
T23589 6011-6015 IN denotes with
T23590 6016-6019 DT denotes the
T23592 6020-6028 JJ denotes parental
T23593 6029-6031 NN denotes B6
T23594 6032-6034 CC denotes or
T23595 6035-6037 NN denotes HG
T23591 6038-6044 NN denotes strain
T23596 6045-6049 IN denotes with
T23597 6050-6053 DT denotes the
T23598 6054-6063 NN denotes exception
T23599 6064-6066 IN denotes of
T23600 6067-6076 NNS denotes mutations
T23601 6077-6081 WDT denotes that
T23602 6082-6087 VBD denotes arose
T23603 6088-6094 IN denotes during
T23604 6095-6103 JJ denotes congenic
T23605 6104-6116 NN denotes construction
T23606 6117-6120 CC denotes and
T23607 6121-6122 DT denotes a
T23609 6123-6128 JJ denotes small
T23608 6129-6139 NN denotes percentage
T23610 6140-6142 IN denotes of
T23611 6143-6156 VBG denotes contaminating
T23613 6157-6162 NN denotes donor
T23612 6163-6170 NNS denotes alleles
T23614 6171-6177 VBN denotes missed
T23615 6178-6183 IN denotes after
T23616 6184-6185 CD denotes 6
T23617 6186-6197 NNS denotes backcrosses
T23618 6197-6198 . denotes .
T23619 6198-6417 sentence denotes Therefore, since the congenics and controls were developed through the same selection scheme and possibly share common contaminating regions, the B6C and HGC strains are the most ideal control to compare each congenic.
T23620 6199-6208 RB denotes Therefore
T23622 6208-6210 , denotes ,
T23623 6210-6215 IN denotes since
T23625 6216-6219 DT denotes the
T23626 6220-6229 NNS denotes congenics
T23627 6230-6233 CC denotes and
T23628 6234-6242 NNS denotes controls
T23629 6243-6247 VBD denotes were
T23624 6248-6257 VBN denotes developed
T23630 6258-6265 IN denotes through
T23631 6266-6269 DT denotes the
T23633 6270-6274 JJ denotes same
T23634 6275-6284 NN denotes selection
T23632 6285-6291 NN denotes scheme
T23635 6292-6295 CC denotes and
T23636 6296-6304 RB denotes possibly
T23637 6305-6310 VBP denotes share
T23638 6311-6317 JJ denotes common
T23640 6318-6331 VBG denotes contaminating
T23639 6332-6339 NNS denotes regions
T23641 6339-6341 , denotes ,
T23642 6341-6344 DT denotes the
T23644 6345-6348 NN denotes B6C
T23645 6349-6352 CC denotes and
T23646 6353-6356 NN denotes HGC
T23643 6357-6364 NNS denotes strains
T23621 6365-6368 VBP denotes are
T23647 6369-6372 DT denotes the
T23649 6373-6377 RBS denotes most
T23650 6378-6383 JJ denotes ideal
T23648 6384-6391 NN denotes control
T23651 6392-6394 TO denotes to
T23652 6395-6402 VB denotes compare
T23653 6403-6407 DT denotes each
T23654 6408-6416 NN denotes congenic
T23655 6416-6417 . denotes .
T24427 6419-6429 JJ denotes Phenotypic
T24428 6430-6446 NN denotes characterization
T24429 6446-6553 sentence denotes Trait data were collected on approximately 40 mice (20 of each sex) from each congenic and control strain.
T24430 6447-6452 NN denotes Trait
T24431 6453-6457 NNS denotes data
T24433 6458-6462 VBD denotes were
T24432 6463-6472 VBN denotes collected
T24434 6473-6475 IN denotes on
T24435 6476-6489 RB denotes approximately
T24436 6490-6492 CD denotes 40
T24437 6493-6497 NNS denotes mice
T24438 6498-6499 -LRB- denotes (
T24439 6499-6501 CD denotes 20
T24440 6502-6504 IN denotes of
T24441 6505-6509 DT denotes each
T24442 6510-6513 NN denotes sex
T24443 6513-6514 -RRB- denotes )
T24444 6515-6519 IN denotes from
T24445 6520-6524 DT denotes each
T24447 6525-6533 JJ denotes congenic
T24448 6534-6537 CC denotes and
T24449 6538-6545 NN denotes control
T24446 6546-6552 NN denotes strain
T24450 6552-6553 . denotes .
T24451 6553-6710 sentence denotes To eliminate parity and reduce litter size effects only progeny from uniparous dams were characterized and all litters were standardized to 5–7 pups/litter.
T24452 6554-6556 TO denotes To
T24453 6557-6566 VB denotes eliminate
T24455 6567-6573 NN denotes parity
T24456 6574-6577 CC denotes and
T24457 6578-6584 VB denotes reduce
T24458 6585-6591 NN denotes litter
T24459 6592-6596 NN denotes size
T24460 6597-6604 NNS denotes effects
T24461 6605-6609 RB denotes only
T24462 6610-6617 NN denotes progeny
T24463 6618-6622 IN denotes from
T24464 6623-6632 JJ denotes uniparous
T24465 6633-6637 NNS denotes dams
T24466 6638-6642 VBD denotes were
T24454 6643-6656 VBN denotes characterized
T24467 6657-6660 CC denotes and
T24468 6661-6664 DT denotes all
T24469 6665-6672 NNS denotes litters
T24471 6673-6677 VBD denotes were
T24470 6678-6690 VBN denotes standardized
T24472 6691-6693 IN denotes to
T24473 6694-6695 CD denotes 5
T24475 6695-6696 SYM denotes –
T24474 6696-6697 CD denotes 7
T24476 6698-6702 NNS denotes pups
T24477 6702-6703 SYM denotes /
T24478 6703-6709 NN denotes litter
T24479 6709-6710 . denotes .
T24480 6710-6746 sentence denotes Mice were weaned at 3 weeks of age.
T24481 6711-6715 NNS denotes Mice
T24483 6716-6720 VBD denotes were
T24482 6721-6727 VBN denotes weaned
T24484 6728-6730 IN denotes at
T24485 6731-6732 CD denotes 3
T24486 6733-6738 NNS denotes weeks
T24487 6739-6741 IN denotes of
T24488 6742-6745 NN denotes age
T24489 6745-6746 . denotes .
T24490 6746-6837 sentence denotes Feed (Purina 5008; 23.5% protein, 6.5% fat, 3.3 Kcal/g) and water were offered ad libitum.
T24491 6747-6751 NN denotes Feed
T24493 6752-6753 -LRB- denotes (
T24495 6753-6759 NNP denotes Purina
T24496 6760-6764 CD denotes 5008
T24497 6764-6765 : denotes ;
T24498 6766-6770 CD denotes 23.5
T24499 6770-6771 NN denotes %
T24494 6772-6779 NN denotes protein
T24500 6779-6781 , denotes ,
T24501 6781-6784 CD denotes 6.5
T24502 6784-6785 NN denotes %
T24503 6786-6789 NN denotes fat
T24504 6789-6791 , denotes ,
T24505 6791-6794 CD denotes 3.3
T24506 6795-6799 NN denotes Kcal
T24507 6799-6800 SYM denotes /
T24508 6800-6801 NN denotes g
T24509 6801-6802 -RRB- denotes )
T24510 6803-6806 CC denotes and
T24511 6807-6812 NN denotes water
T24512 6813-6817 VBD denotes were
T24492 6818-6825 VBN denotes offered
T24513 6826-6828 FW denotes ad
T24514 6829-6836 FW denotes libitum
T24515 6836-6837 . denotes .
T24516 6837-6910 sentence denotes Mice were weighed to the nearest 0.1 g at 2WK, 3WK, 6WK, and 9WK of age.
T24517 6838-6842 NNS denotes Mice
T24519 6843-6847 VBD denotes were
T24518 6848-6855 VBN denotes weighed
T24520 6856-6858 IN denotes to
T24521 6859-6862 DT denotes the
T24523 6863-6870 JJS denotes nearest
T24524 6871-6874 CD denotes 0.1
T24522 6875-6876 NN denotes g
T24525 6877-6879 IN denotes at
T24526 6880-6883 NN denotes 2WK
T24527 6883-6885 , denotes ,
T24528 6885-6888 NN denotes 3WK
T24529 6888-6890 , denotes ,
T24530 6890-6893 NN denotes 6WK
T24531 6893-6895 , denotes ,
T24532 6895-6898 CC denotes and
T24533 6899-6902 NN denotes 9WK
T24534 6903-6905 IN denotes of
T24535 6906-6909 NN denotes age
T24536 6909-6910 . denotes .
T24537 6910-7049 sentence denotes At 9WK ( ± 5 days) mice were anesthetized under isoflurane and nasal-anal length (NA) and nasal-tail (NT) were measured to the nearest mm.
T24538 6911-6913 IN denotes At
T24540 6914-6917 NN denotes 9WK
T24541 6918-6919 -LRB- denotes (
T24542 6920-6921 SYM denotes ±
T24543 6922-6923 CD denotes 5
T24544 6924-6928 NNS denotes days
T24545 6928-6929 -RRB- denotes )
T24546 6930-6934 NNS denotes mice
T24547 6935-6939 VBD denotes were
T24539 6940-6952 VBN denotes anesthetized
T24548 6953-6958 IN denotes under
T24549 6959-6969 NN denotes isoflurane
T24550 6970-6973 CC denotes and
T24551 6974-6979 JJ denotes nasal
T24553 6979-6980 HYPH denotes -
T24552 6980-6984 JJ denotes anal
T24554 6985-6991 NN denotes length
T24556 6992-6993 -LRB- denotes (
T24557 6993-6995 NN denotes NA
T24558 6995-6996 -RRB- denotes )
T24559 6997-7000 CC denotes and
T24560 7001-7006 JJ denotes nasal
T24562 7006-7007 HYPH denotes -
T24561 7007-7011 NN denotes tail
T24563 7012-7013 -LRB- denotes (
T24564 7013-7015 NN denotes NT
T24565 7015-7016 -RRB- denotes )
T24566 7017-7021 VBD denotes were
T24555 7022-7030 VBN denotes measured
T24567 7031-7033 IN denotes to
T24568 7034-7037 DT denotes the
T24570 7038-7045 JJS denotes nearest
T24569 7046-7048 NN denotes mm
T24571 7048-7049 . denotes .
T24572 7049-7092 sentence denotes Tail length was calculated as NA minus NT.
T24573 7050-7054 NN denotes Tail
T24574 7055-7061 NN denotes length
T24576 7062-7065 VBD denotes was
T24575 7066-7076 VBN denotes calculated
T24577 7077-7079 IN denotes as
T24578 7080-7082 NN denotes NA
T24579 7083-7088 CC denotes minus
T24580 7089-7091 NN denotes NT
T24581 7091-7092 . denotes .
T24582 7092-7166 sentence denotes Anesthetized mice were then sacrificed by decapitation and exsanguinated.
T24583 7093-7105 VBN denotes Anesthetized
T24584 7106-7110 NNS denotes mice
T24586 7111-7115 VBD denotes were
T24587 7116-7120 RB denotes then
T24585 7121-7131 VBN denotes sacrificed
T24588 7132-7134 IN denotes by
T24589 7135-7147 NN denotes decapitation
T24590 7148-7151 CC denotes and
T24591 7152-7165 VBN denotes exsanguinated
T24592 7165-7166 . denotes .
T24593 7166-7315 sentence denotes Femoral fat pad (FFP), gonadal fat pad (GFP), mesenteric fat pad (MFP) and retroperitoneal fat pad (RFP) were removed and weighed to the nearest mg.
T24594 7167-7174 JJ denotes Femoral
T24596 7175-7178 NN denotes fat
T24595 7179-7182 NN denotes pad
T24598 7183-7184 -LRB- denotes (
T24599 7184-7187 NN denotes FFP
T24600 7187-7188 -RRB- denotes )
T24601 7188-7190 , denotes ,
T24602 7190-7197 JJ denotes gonadal
T24604 7198-7201 NN denotes fat
T24603 7202-7205 NN denotes pad
T24605 7206-7207 -LRB- denotes (
T24606 7207-7210 NN denotes GFP
T24607 7210-7211 -RRB- denotes )
T24608 7211-7213 , denotes ,
T24609 7213-7223 JJ denotes mesenteric
T24611 7224-7227 NN denotes fat
T24610 7228-7231 NN denotes pad
T24612 7232-7233 -LRB- denotes (
T24613 7233-7236 NN denotes MFP
T24614 7236-7237 -RRB- denotes )
T24615 7238-7241 CC denotes and
T24616 7242-7257 JJ denotes retroperitoneal
T24618 7258-7261 NN denotes fat
T24617 7262-7265 NN denotes pad
T24619 7266-7267 -LRB- denotes (
T24620 7267-7270 NN denotes RFP
T24621 7270-7271 -RRB- denotes )
T24622 7272-7276 VBD denotes were
T24597 7277-7284 VBN denotes removed
T24623 7285-7288 CC denotes and
T24624 7289-7296 VBN denotes weighed
T24625 7297-7299 IN denotes to
T24626 7300-7303 DT denotes the
T24628 7304-7311 JJS denotes nearest
T24627 7312-7314 NN denotes mg
T24629 7314-7315 . denotes .
T24630 7315-7450 sentence denotes Chemical compositional analysis was performed for HGC, HG11 and HG17 carcasses as previously described with slight modifications [24].
T24631 7316-7324 JJ denotes Chemical
T24633 7325-7338 JJ denotes compositional
T24632 7339-7347 NN denotes analysis
T24635 7348-7351 VBD denotes was
T24634 7352-7361 VBN denotes performed
T24636 7362-7365 IN denotes for
T24637 7366-7369 NN denotes HGC
T24639 7369-7371 , denotes ,
T24640 7371-7375 NN denotes HG11
T24641 7376-7379 CC denotes and
T24642 7380-7384 NN denotes HG17
T24638 7385-7394 NNS denotes carcasses
T24643 7395-7397 IN denotes as
T24645 7398-7408 RB denotes previously
T24644 7409-7418 VBN denotes described
T24646 7419-7423 IN denotes with
T24647 7424-7430 JJ denotes slight
T24648 7431-7444 NNS denotes modifications
T24649 7445-7446 -LRB- denotes [
T24650 7446-7448 CD denotes 24
T24651 7448-7449 -RRB- denotes ]
T24652 7449-7450 . denotes .
T24653 7450-7516 sentence denotes Briefly, after weighing each fat pad was returned to the carcass.
T24654 7451-7458 RB denotes Briefly
T24656 7458-7460 , denotes ,
T24657 7460-7465 IN denotes after
T24658 7466-7474 VBG denotes weighing
T24659 7475-7479 DT denotes each
T24661 7480-7483 NN denotes fat
T24660 7484-7487 NN denotes pad
T24662 7488-7491 VBD denotes was
T24655 7492-7500 VBN denotes returned
T24663 7501-7503 IN denotes to
T24664 7504-7507 DT denotes the
T24665 7508-7515 NN denotes carcass
T24666 7515-7516 . denotes .
T24667 7516-7614 sentence denotes The entire gastrointestinal (GI) tract was subsequently removed and carcasses were again weighed.
T24668 7517-7520 DT denotes The
T24670 7521-7527 JJ denotes entire
T24671 7528-7544 JJ denotes gastrointestinal
T24672 7545-7546 -LRB- denotes (
T24673 7546-7548 NN denotes GI
T24674 7548-7549 -RRB- denotes )
T24669 7550-7555 NN denotes tract
T24676 7556-7559 VBD denotes was
T24677 7560-7572 RB denotes subsequently
T24675 7573-7580 VBN denotes removed
T24678 7581-7584 CC denotes and
T24679 7585-7594 NNS denotes carcasses
T24681 7595-7599 VBD denotes were
T24682 7600-7605 RB denotes again
T24680 7606-7613 VBN denotes weighed
T24683 7613-7614 . denotes .
T24684 7614-7663 sentence denotes This represented the empty carcass weight (ECW).
T24685 7615-7619 DT denotes This
T24686 7620-7631 VBD denotes represented
T24687 7632-7635 DT denotes the
T24689 7636-7641 JJ denotes empty
T24690 7642-7649 NN denotes carcass
T24688 7650-7656 NN denotes weight
T24691 7657-7658 -LRB- denotes (
T24692 7658-7661 NN denotes ECW
T24693 7661-7662 -RRB- denotes )
T24694 7662-7663 . denotes .
T24695 7663-7772 sentence denotes Carcasses were labeled and secured in two layers of cheesecloth (Fisher) and frozen at -20°C until analysis.
T24696 7664-7673 NNS denotes Carcasses
T24698 7674-7678 VBD denotes were
T24697 7679-7686 VBN denotes labeled
T24699 7687-7690 CC denotes and
T24700 7691-7698 VBN denotes secured
T24701 7699-7701 IN denotes in
T24702 7702-7705 CD denotes two
T24703 7706-7712 NNS denotes layers
T24704 7713-7715 IN denotes of
T24705 7716-7727 NN denotes cheesecloth
T24706 7728-7729 -LRB- denotes (
T24707 7729-7735 NNP denotes Fisher
T24708 7735-7736 -RRB- denotes )
T24709 7737-7740 CC denotes and
T24710 7741-7747 VBN denotes frozen
T24711 7748-7750 IN denotes at
T24712 7751-7752 SYM denotes -
T24713 7752-7754 CD denotes 20
T24714 7754-7756 NN denotes °C
T24715 7757-7762 IN denotes until
T24716 7763-7771 NN denotes analysis
T24717 7771-7772 . denotes .
T24718 7772-7911 sentence denotes At this time, carcasses were freeze-dried for seven days and water content was determined by subtracting the freeze-dried weight from ECW.
T24719 7773-7775 IN denotes At
T24721 7776-7780 DT denotes this
T24722 7781-7785 NN denotes time
T24723 7785-7787 , denotes ,
T24724 7787-7796 NNS denotes carcasses
T24725 7797-7801 VBD denotes were
T24726 7802-7808 NN denotes freeze
T24727 7808-7809 HYPH denotes -
T24720 7809-7814 VBN denotes dried
T24728 7815-7818 IN denotes for
T24729 7819-7824 CD denotes seven
T24730 7825-7829 NNS denotes days
T24731 7830-7833 CC denotes and
T24732 7834-7839 NN denotes water
T24733 7840-7847 NN denotes content
T24735 7848-7851 VBD denotes was
T24734 7852-7862 VBN denotes determined
T24736 7863-7865 IN denotes by
T24737 7866-7877 VBG denotes subtracting
T24738 7878-7881 DT denotes the
T24740 7882-7888 NN denotes freeze
T24742 7888-7889 HYPH denotes -
T24741 7889-7894 VBN denotes dried
T24739 7895-7901 NN denotes weight
T24743 7902-7906 IN denotes from
T24744 7907-7910 NNP denotes ECW
T24745 7910-7911 . denotes .
T24746 7911-8026 sentence denotes FAT was then extracted with ether for 7 days, followed by acetone for an additional 7 days in a Soxhlet apparatus.
T24747 7912-7915 NN denotes FAT
T24749 7916-7919 VBD denotes was
T24750 7920-7924 RB denotes then
T24751 7925-7934 VBN denotes extracted
T24752 7935-7939 IN denotes with
T24753 7940-7945 NN denotes ether
T24754 7946-7949 IN denotes for
T24755 7950-7951 CD denotes 7
T24756 7952-7956 NNS denotes days
T24757 7956-7958 , denotes ,
T24748 7958-7966 VBN denotes followed
T24758 7967-7969 IN denotes by
T24759 7970-7977 NN denotes acetone
T24760 7978-7981 IN denotes for
T24761 7982-7984 DT denotes an
T24763 7985-7995 JJ denotes additional
T24764 7996-7997 CD denotes 7
T24762 7998-8002 NNS denotes days
T24765 8003-8005 IN denotes in
T24766 8006-8007 DT denotes a
T24768 8008-8015 NNP denotes Soxhlet
T24767 8016-8025 NN denotes apparatus
T24769 8025-8026 . denotes .
T24770 8026-8120 sentence denotes Carcass ash (ASH) was determined measuring the remains after a 16 hour incineration at 575°C.
T24771 8027-8034 NN denotes Carcass
T24772 8035-8038 NN denotes ash
T24774 8039-8040 -LRB- denotes (
T24775 8040-8043 NN denotes ASH
T24776 8043-8044 -RRB- denotes )
T24777 8045-8048 VBD denotes was
T24773 8049-8059 VBN denotes determined
T24778 8060-8069 VBG denotes measuring
T24779 8070-8073 DT denotes the
T24780 8074-8081 NNS denotes remains
T24781 8082-8087 IN denotes after
T24782 8088-8089 DT denotes a
T24784 8090-8092 CD denotes 16
T24785 8093-8097 NN denotes hour
T24783 8098-8110 NN denotes incineration
T24786 8111-8113 IN denotes at
T24787 8114-8117 CD denotes 575
T24788 8117-8119 NN denotes °C
T24789 8119-8120 . denotes .
T24790 8120-8241 sentence denotes Carcass protein (PROT) was calculated as the remaining portion after carcass fat (FAT) and ASH were subtracted from ECW.
T24791 8121-8128 NN denotes Carcass
T24792 8129-8136 NN denotes protein
T24794 8137-8138 -LRB- denotes (
T24795 8138-8142 NN denotes PROT
T24796 8142-8143 -RRB- denotes )
T24797 8144-8147 VBD denotes was
T24793 8148-8158 VBN denotes calculated
T24798 8159-8161 IN denotes as
T24799 8162-8165 DT denotes the
T24801 8166-8175 VBG denotes remaining
T24800 8176-8183 NN denotes portion
T24802 8184-8189 IN denotes after
T24804 8190-8197 NN denotes carcass
T24805 8198-8201 NN denotes fat
T24806 8202-8203 -LRB- denotes (
T24807 8203-8206 NN denotes FAT
T24808 8206-8207 -RRB- denotes )
T24809 8208-8211 CC denotes and
T24810 8212-8215 NN denotes ASH
T24811 8216-8220 VBD denotes were
T24803 8221-8231 VBN denotes subtracted
T24812 8232-8236 IN denotes from
T24813 8237-8240 NN denotes ECW
T24814 8240-8241 . denotes .
T25106 8243-8254 JJ denotes Statistical
T25107 8255-8263 NN denotes analysis
T25108 8263-8403 sentence denotes The MEANS and UNIVARIATE procedures of SAS were used to generate descriptive statistics and test normality assumptions for each trait [55].
T25109 8264-8267 DT denotes The
T25111 8268-8273 NN denotes MEANS
T25112 8274-8277 CC denotes and
T25113 8278-8288 NN denotes UNIVARIATE
T25110 8289-8299 NNS denotes procedures
T25115 8300-8302 IN denotes of
T25116 8303-8306 NNP denotes SAS
T25117 8307-8311 VBD denotes were
T25114 8312-8316 VBN denotes used
T25118 8317-8319 TO denotes to
T25119 8320-8328 VB denotes generate
T25120 8329-8340 JJ denotes descriptive
T25121 8341-8351 NNS denotes statistics
T25122 8352-8355 CC denotes and
T25123 8356-8360 VB denotes test
T25124 8361-8370 NN denotes normality
T25125 8371-8382 NNS denotes assumptions
T25126 8383-8386 IN denotes for
T25127 8387-8391 DT denotes each
T25128 8392-8397 NN denotes trait
T25129 8398-8399 -LRB- denotes [
T25130 8399-8401 CD denotes 55
T25131 8401-8402 -RRB- denotes ]
T25132 8402-8403 . denotes .
T25133 8403-8625 sentence denotes All data were then analyzed using the GLM procedure of SAS [55] with a linear model that included the fixed effects of strain, sex and strain by sex interaction; dam's weight at breeding by strain was used as a covariate.
T25134 8404-8407 DT denotes All
T25135 8408-8412 NNS denotes data
T25137 8413-8417 VBD denotes were
T25138 8418-8422 RB denotes then
T25136 8423-8431 VBN denotes analyzed
T25140 8432-8437 VBG denotes using
T25141 8438-8441 DT denotes the
T25143 8442-8445 NN denotes GLM
T25142 8446-8455 NN denotes procedure
T25144 8456-8458 IN denotes of
T25145 8459-8462 NN denotes SAS
T25146 8463-8464 -LRB- denotes [
T25147 8464-8466 CD denotes 55
T25148 8466-8467 -RRB- denotes ]
T25149 8468-8472 IN denotes with
T25150 8473-8474 DT denotes a
T25152 8475-8481 JJ denotes linear
T25151 8482-8487 NN denotes model
T25153 8488-8492 WDT denotes that
T25154 8493-8501 VBD denotes included
T25155 8502-8505 DT denotes the
T25157 8506-8511 VBN denotes fixed
T25156 8512-8519 NNS denotes effects
T25158 8520-8522 IN denotes of
T25159 8523-8529 NN denotes strain
T25160 8529-8531 , denotes ,
T25161 8531-8534 NN denotes sex
T25162 8535-8538 CC denotes and
T25163 8539-8545 NN denotes strain
T25165 8546-8548 IN denotes by
T25166 8549-8552 NN denotes sex
T25164 8553-8564 NN denotes interaction
T25167 8564-8565 : denotes ;
T25168 8566-8569 NN denotes dam
T25170 8569-8571 POS denotes 's
T25169 8572-8578 NN denotes weight
T25171 8579-8581 IN denotes at
T25172 8582-8590 NN denotes breeding
T25173 8591-8593 IN denotes by
T25174 8594-8600 NN denotes strain
T25175 8601-8604 VBD denotes was
T25139 8605-8609 VBN denotes used
T25176 8610-8612 IN denotes as
T25177 8613-8614 DT denotes a
T25178 8615-8624 NN denotes covariate
T25179 8624-8625 . denotes .
T25180 8625-8719 sentence denotes A second linear model was used to test for strain by hg genotype (+/+ or hg/hg) interactions.
T25181 8626-8627 DT denotes A
T25183 8628-8634 JJ denotes second
T25184 8635-8641 JJ denotes linear
T25182 8642-8647 NN denotes model
T25186 8648-8651 VBD denotes was
T25185 8652-8656 VBN denotes used
T25187 8657-8659 TO denotes to
T25188 8660-8664 VB denotes test
T25189 8665-8668 IN denotes for
T25190 8669-8675 NN denotes strain
T25192 8676-8678 IN denotes by
T25193 8679-8681 NN denotes hg
T25194 8682-8690 NN denotes genotype
T25195 8691-8692 -LRB- denotes (
T25197 8692-8693 SYM denotes +
T25198 8693-8694 HYPH denotes /
T25196 8694-8695 SYM denotes +
T25199 8696-8698 CC denotes or
T25200 8699-8701 NN denotes hg
T25202 8701-8702 HYPH denotes /
T25201 8702-8704 NN denotes hg
T25203 8704-8705 -RRB- denotes )
T25191 8706-8718 NNS denotes interactions
T25204 8718-8719 . denotes .
T25205 8719-8843 sentence denotes This model included the fixed effects of donor region, sex and HG genotype and all possible two and three-way interactions.
T25206 8720-8724 DT denotes This
T25207 8725-8730 NN denotes model
T25208 8731-8739 VBD denotes included
T25209 8740-8743 DT denotes the
T25211 8744-8749 VBN denotes fixed
T25210 8750-8757 NNS denotes effects
T25212 8758-8760 IN denotes of
T25213 8761-8766 NN denotes donor
T25214 8767-8773 NN denotes region
T25215 8773-8775 , denotes ,
T25216 8775-8778 NN denotes sex
T25217 8779-8782 CC denotes and
T25218 8783-8785 NN denotes HG
T25219 8786-8794 NN denotes genotype
T25220 8795-8798 CC denotes and
T25221 8799-8802 DT denotes all
T25223 8803-8811 JJ denotes possible
T25224 8812-8815 CD denotes two
T25226 8816-8819 CC denotes and
T25227 8820-8825 CD denotes three
T25228 8825-8826 HYPH denotes -
T25225 8826-8829 NN denotes way
T25222 8830-8842 NNS denotes interactions
T25229 8842-8843 . denotes .
T25230 8843-8994 sentence denotes Choosing a nominal P value of 0.05 and applying the Bonferroni correction for multiple comparisons established significant differences in the ANOVA's.
T25231 8844-8852 VBG denotes Choosing
T25233 8853-8854 DT denotes a
T25235 8855-8862 JJ denotes nominal
T25236 8863-8864 NN denotes P
T25234 8865-8870 NN denotes value
T25237 8871-8873 IN denotes of
T25238 8874-8878 CD denotes 0.05
T25239 8879-8882 CC denotes and
T25240 8883-8891 VBG denotes applying
T25241 8892-8895 DT denotes the
T25243 8896-8906 NNP denotes Bonferroni
T25242 8907-8917 NN denotes correction
T25244 8918-8921 IN denotes for
T25245 8922-8930 JJ denotes multiple
T25246 8931-8942 NNS denotes comparisons
T25232 8943-8954 VBD denotes established
T25247 8955-8966 JJ denotes significant
T25248 8967-8978 NNS denotes differences
T25249 8979-8981 IN denotes in
T25250 8982-8985 DT denotes the
T25251 8986-8991 NNP denotes ANOVA
T25252 8991-8993 AFX denotes 's
T25253 8993-8994 . denotes .
T25254 8994-9050 sentence denotes The critical P values used are indicated in each table.
T25255 8995-8998 DT denotes The
T25257 8999-9007 JJ denotes critical
T25258 9008-9009 NN denotes P
T25256 9010-9016 NNS denotes values
T25260 9017-9021 VBN denotes used
T25261 9022-9025 VBP denotes are
T25259 9026-9035 VBN denotes indicated
T25262 9036-9038 IN denotes in
T25263 9039-9043 DT denotes each
T25264 9044-9049 NN denotes table
T25265 9049-9050 . denotes .
T25440 9052-9066 NN denotes Identification
T25441 9067-9069 IN denotes of
T25442 9070-9079 NN denotes candidate
T25443 9080-9085 NNS denotes genes
T25444 9085-9179 sentence denotes Genes were identified by manual data mining of primary literature, reviews and book chapters.
T25445 9086-9091 NNS denotes Genes
T25447 9092-9096 VBD denotes were
T25446 9097-9107 VBN denotes identified
T25448 9108-9110 IN denotes by
T25449 9111-9117 JJ denotes manual
T25451 9118-9122 NNS denotes data
T25450 9123-9129 NN denotes mining
T25452 9130-9132 IN denotes of
T25453 9133-9140 JJ denotes primary
T25454 9141-9151 NN denotes literature
T25455 9151-9153 , denotes ,
T25456 9153-9160 NNS denotes reviews
T25457 9161-9164 CC denotes and
T25458 9165-9169 NN denotes book
T25459 9170-9178 NNS denotes chapters
T25460 9178-9179 . denotes .
T25461 9179-9360 sentence denotes To organize and collate genomic and functional information for each gene we created a custom Gh signaling Gene Map Annotator and Pathway Profiler (GenMAPP) pathway [56] (Figure 5).
T25462 9180-9182 TO denotes To
T25463 9183-9191 VB denotes organize
T25465 9192-9195 CC denotes and
T25466 9196-9203 VB denotes collate
T25467 9204-9211 JJ denotes genomic
T25469 9212-9215 CC denotes and
T25470 9216-9226 JJ denotes functional
T25468 9227-9238 NN denotes information
T25471 9239-9242 IN denotes for
T25472 9243-9247 DT denotes each
T25473 9248-9252 NN denotes gene
T25474 9253-9255 PRP denotes we
T25464 9256-9263 VBD denotes created
T25475 9264-9265 DT denotes a
T25477 9266-9272 NN denotes custom
T25478 9273-9275 NN denotes Gh
T25479 9276-9285 NN denotes signaling
T25480 9286-9290 NN denotes Gene
T25481 9291-9294 NN denotes Map
T25476 9295-9304 NN denotes Annotator
T25482 9305-9308 CC denotes and
T25483 9309-9316 NN denotes Pathway
T25484 9317-9325 NN denotes Profiler
T25486 9326-9327 -LRB- denotes (
T25487 9327-9334 NNP denotes GenMAPP
T25488 9334-9335 -RRB- denotes )
T25485 9336-9343 NN denotes pathway
T25489 9344-9345 -LRB- denotes [
T25490 9345-9347 CD denotes 56
T25491 9347-9348 -RRB- denotes ]
T25492 9349-9350 -LRB- denotes (
T25493 9350-9356 NN denotes Figure
T25494 9357-9358 CD denotes 5
T25495 9358-9359 -RRB- denotes )
T25496 9359-9360 . denotes .
T25497 9360-9503 sentence denotes Visualization and color-coding of genes using GenMAPP aided the selection of candidate genes mapping within QTL regions on MMU2, 9, 11 and 17.
T25498 9361-9374 NN denotes Visualization
T25500 9375-9378 CC denotes and
T25501 9379-9384 NN denotes color
T25502 9384-9385 HYPH denotes -
T25503 9385-9391 VBG denotes coding
T25504 9392-9394 IN denotes of
T25505 9395-9400 NNS denotes genes
T25506 9401-9406 VBG denotes using
T25507 9407-9414 NNP denotes GenMAPP
T25499 9415-9420 VBD denotes aided
T25508 9421-9424 DT denotes the
T25509 9425-9434 NN denotes selection
T25510 9435-9437 IN denotes of
T25511 9438-9447 NN denotes candidate
T25512 9448-9453 NNS denotes genes
T25513 9454-9461 VBG denotes mapping
T25514 9462-9468 IN denotes within
T25515 9469-9472 NNS denotes QTL
T25516 9473-9480 NNS denotes regions
T25517 9481-9483 IN denotes on
T25518 9484-9487 NN denotes MMU
T25519 9487-9488 CD denotes 2
T25520 9488-9490 , denotes ,
T25521 9490-9491 CD denotes 9
T25522 9491-9493 , denotes ,
T25523 9493-9495 CD denotes 11
T25524 9496-9499 CC denotes and
T25525 9500-9502 CD denotes 17
T25526 9502-9503 . denotes .
T26350 9505-9514 NN denotes Candidate
T26351 9515-9519 NN denotes gene
T26352 9520-9530 NN denotes sequencing
T26353 9530-9769 sentence denotes PCR amplicons covering the coding sequence and partial 5' and 3' untranslated regions for each selected candidate gene were amplified, purified and sequenced from the CAST strain using protocols outlined in [57] with slight modifications.
T26354 9531-9534 NN denotes PCR
T26355 9535-9544 NNS denotes amplicons
T26357 9545-9553 VBG denotes covering
T26358 9554-9557 DT denotes the
T26360 9558-9564 NN denotes coding
T26359 9565-9573 NN denotes sequence
T26361 9574-9577 CC denotes and
T26362 9578-9585 JJ denotes partial
T26364 9586-9587 CD denotes 5
T26365 9587-9588 SYM denotes '
T26366 9589-9592 CC denotes and
T26367 9593-9594 CD denotes 3
T26368 9594-9595 SYM denotes '
T26369 9596-9608 JJ denotes untranslated
T26363 9609-9616 NNS denotes regions
T26370 9617-9620 IN denotes for
T26371 9621-9625 DT denotes each
T26373 9626-9634 VBN denotes selected
T26374 9635-9644 NN denotes candidate
T26372 9645-9649 NN denotes gene
T26375 9650-9654 VBD denotes were
T26356 9655-9664 VBN denotes amplified
T26376 9664-9666 , denotes ,
T26377 9666-9674 VBN denotes purified
T26378 9675-9678 CC denotes and
T26379 9679-9688 VBN denotes sequenced
T26380 9689-9693 IN denotes from
T26381 9694-9697 DT denotes the
T26383 9698-9702 NN denotes CAST
T26382 9703-9709 NN denotes strain
T26384 9710-9715 VBG denotes using
T26385 9716-9725 NNS denotes protocols
T26386 9726-9734 VBN denotes outlined
T26387 9735-9737 IN denotes in
T26388 9738-9739 -LRB- denotes [
T26389 9739-9741 CD denotes 57
T26390 9741-9742 -RRB- denotes ]
T26391 9743-9747 IN denotes with
T26392 9748-9754 JJ denotes slight
T26393 9755-9768 NNS denotes modifications
T26394 9768-9769 . denotes .
T26395 9769-9888 sentence denotes Total RNA from brain, liver, spleen, lung and testis was isolated from an adult CAST male mouse using Trizol (Ambion).
T26396 9770-9775 JJ denotes Total
T26397 9776-9779 NN denotes RNA
T26399 9780-9784 IN denotes from
T26400 9785-9790 NN denotes brain
T26401 9790-9792 , denotes ,
T26402 9792-9797 NN denotes liver
T26403 9797-9799 , denotes ,
T26404 9799-9805 NN denotes spleen
T26405 9805-9807 , denotes ,
T26406 9807-9811 NN denotes lung
T26407 9812-9815 CC denotes and
T26408 9816-9822 NN denotes testis
T26409 9823-9826 VBD denotes was
T26398 9827-9835 VBN denotes isolated
T26410 9836-9840 IN denotes from
T26411 9841-9843 DT denotes an
T26413 9844-9849 JJ denotes adult
T26414 9850-9854 NN denotes CAST
T26415 9855-9859 JJ denotes male
T26412 9860-9865 NN denotes mouse
T26416 9866-9871 VBG denotes using
T26417 9872-9878 NN denotes Trizol
T26418 9879-9880 -LRB- denotes (
T26419 9880-9886 NNP denotes Ambion
T26420 9886-9887 -RRB- denotes )
T26421 9887-9888 . denotes .
T26422 9888-9948 sentence denotes cDNA was produced from total RNA using standard procedures.
T26423 9889-9893 NN denotes cDNA
T26425 9894-9897 VBD denotes was
T26424 9898-9906 VBN denotes produced
T26426 9907-9911 IN denotes from
T26427 9912-9917 JJ denotes total
T26428 9918-9921 NN denotes RNA
T26429 9922-9927 VBG denotes using
T26430 9928-9936 JJ denotes standard
T26431 9937-9947 NNS denotes procedures
T26432 9947-9948 . denotes .
T26433 9948-10032 sentence denotes PCR primer sets for each gene were designed using Primer3 [58] (Additional File 5).
T26434 9949-9952 NN denotes PCR
T26436 9953-9959 NN denotes primer
T26435 9960-9964 NNS denotes sets
T26438 9965-9968 IN denotes for
T26439 9969-9973 DT denotes each
T26440 9974-9978 NN denotes gene
T26441 9979-9983 VBD denotes were
T26437 9984-9992 VBN denotes designed
T26442 9993-9998 VBG denotes using
T26443 9999-10006 NN denotes Primer3
T26444 10007-10008 -LRB- denotes [
T26445 10008-10010 CD denotes 58
T26446 10010-10011 -RRB- denotes ]
T26447 10012-10013 -LRB- denotes (
T26449 10013-10023 JJ denotes Additional
T26448 10024-10028 NN denotes File
T26450 10029-10030 CD denotes 5
T26451 10030-10031 -RRB- denotes )
T26452 10031-10032 . denotes .
T26453 10032-10168 sentence denotes The initial amplification was performed in 10 μl PCR reactions using the Invitrogen Platinum TaqPCRx amplification system (Invitrogen).
T26454 10033-10036 DT denotes The
T26456 10037-10044 JJ denotes initial
T26455 10045-10058 NN denotes amplification
T26458 10059-10062 VBD denotes was
T26457 10063-10072 VBN denotes performed
T26459 10073-10075 IN denotes in
T26460 10076-10078 CD denotes 10
T26461 10079-10081 NN denotes μl
T26463 10082-10085 NN denotes PCR
T26462 10086-10095 NNS denotes reactions
T26464 10096-10101 VBG denotes using
T26465 10102-10105 DT denotes the
T26467 10106-10116 NNP denotes Invitrogen
T26468 10117-10125 NN denotes Platinum
T26469 10126-10133 NN denotes TaqPCRx
T26470 10134-10147 NN denotes amplification
T26466 10148-10154 NN denotes system
T26471 10155-10156 -LRB- denotes (
T26472 10156-10166 NNP denotes Invitrogen
T26473 10166-10167 -RRB- denotes )
T26474 10167-10168 . denotes .
T26475 10168-10347 sentence denotes The reactions contained 1X PCR buffer, 1X Enhancer solution, 1.5 mM MgSO4, 0.17 mM dNTPs, 1 μM each primer, 0.1 unit of Platinum Taq (Invitrogen) and approximately 25 ng of cDNA.
T26476 10169-10172 DT denotes The
T26477 10173-10182 NNS denotes reactions
T26478 10183-10192 VBD denotes contained
T26479 10193-10195 NN denotes 1X
T26481 10196-10199 NN denotes PCR
T26480 10200-10206 NN denotes buffer
T26482 10206-10208 , denotes ,
T26483 10208-10210 NN denotes 1X
T26485 10211-10219 NN denotes Enhancer
T26484 10220-10228 NN denotes solution
T26486 10228-10230 , denotes ,
T26487 10230-10233 CD denotes 1.5
T26488 10234-10236 NN denotes mM
T26489 10237-10242 NN denotes MgSO4
T26490 10242-10244 , denotes ,
T26491 10244-10248 CD denotes 0.17
T26492 10249-10251 NN denotes mM
T26493 10252-10257 NNS denotes dNTPs
T26494 10257-10259 , denotes ,
T26495 10259-10260 CD denotes 1
T26496 10261-10263 NN denotes μM
T26498 10264-10268 DT denotes each
T26497 10269-10275 NN denotes primer
T26499 10275-10277 , denotes ,
T26500 10277-10280 CD denotes 0.1
T26501 10281-10285 NN denotes unit
T26502 10286-10288 IN denotes of
T26503 10289-10297 NN denotes Platinum
T26504 10298-10301 NN denotes Taq
T26505 10302-10303 -LRB- denotes (
T26506 10303-10313 NNP denotes Invitrogen
T26507 10313-10314 -RRB- denotes )
T26508 10315-10318 CC denotes and
T26509 10319-10332 RB denotes approximately
T26510 10333-10335 CD denotes 25
T26511 10336-10338 NN denotes ng
T26512 10339-10341 IN denotes of
T26513 10342-10346 NN denotes cDNA
T26514 10346-10347 . denotes .
T26515 10347-10528 sentence denotes Reactions were incubated for 5 min at 95°C, then cycled for 45 s at 95°C, 45 s at 55°C, 1 min at 72°C for 35 cycles with a final 72°C extension for 10 min on a MJ Research PTC-200.
T26516 10348-10357 NNS denotes Reactions
T26518 10358-10362 VBD denotes were
T26517 10363-10372 VBN denotes incubated
T26519 10373-10376 IN denotes for
T26520 10377-10378 CD denotes 5
T26521 10379-10382 NN denotes min
T26522 10383-10385 IN denotes at
T26523 10386-10388 CD denotes 95
T26524 10388-10390 NN denotes °C
T26525 10390-10392 , denotes ,
T26526 10392-10396 RB denotes then
T26527 10397-10403 VBN denotes cycled
T26528 10404-10407 IN denotes for
T26529 10408-10410 CD denotes 45
T26530 10411-10412 NN denotes s
T26531 10413-10415 IN denotes at
T26532 10416-10418 CD denotes 95
T26533 10418-10420 NN denotes °C
T26534 10420-10422 , denotes ,
T26535 10422-10424 CD denotes 45
T26536 10425-10426 NN denotes s
T26537 10427-10429 IN denotes at
T26538 10430-10432 CD denotes 55
T26539 10432-10434 NN denotes °C
T26540 10434-10436 , denotes ,
T26541 10436-10437 CD denotes 1
T26542 10438-10441 NN denotes min
T26543 10442-10444 IN denotes at
T26544 10445-10447 CD denotes 72
T26545 10447-10449 NN denotes °C
T26546 10450-10453 IN denotes for
T26547 10454-10456 CD denotes 35
T26548 10457-10463 NNS denotes cycles
T26549 10464-10468 IN denotes with
T26550 10469-10470 DT denotes a
T26552 10471-10476 JJ denotes final
T26553 10477-10479 CD denotes 72
T26554 10479-10481 NN denotes °C
T26551 10482-10491 NN denotes extension
T26555 10492-10495 IN denotes for
T26556 10496-10498 CD denotes 10
T26557 10499-10502 NN denotes min
T26558 10503-10505 IN denotes on
T26559 10506-10507 DT denotes a
T26561 10508-10510 NNP denotes MJ
T26562 10511-10519 NNP denotes Research
T26560 10520-10523 NNP denotes PTC
T26563 10523-10524 HYPH denotes -
T26564 10524-10527 CD denotes 200
T26565 10527-10528 . denotes .
T26566 10528-10606 sentence denotes The products were visualized on 1.5% agarose gels containing 0.06 μg/ml EtBr.
T26567 10529-10532 DT denotes The
T26568 10533-10541 NNS denotes products
T26570 10542-10546 VBD denotes were
T26569 10547-10557 VBN denotes visualized
T26571 10558-10560 IN denotes on
T26572 10561-10564 CD denotes 1.5
T26573 10564-10565 NN denotes %
T26574 10566-10573 NN denotes agarose
T26575 10574-10578 NNS denotes gels
T26576 10579-10589 VBG denotes containing
T26577 10590-10594 CD denotes 0.06
T26578 10595-10597 NN denotes μg
T26580 10597-10598 SYM denotes /
T26581 10598-10600 NN denotes ml
T26579 10601-10605 NN denotes EtBr
T26582 10605-10606 . denotes .
T26583 10606-10717 sentence denotes Bands of the correct size were excised and incubated in 100 μl of sterile H2O at 80°C for 10 min to elute DNA.
T26584 10607-10612 NNS denotes Bands
T26586 10613-10615 IN denotes of
T26587 10616-10619 DT denotes the
T26589 10620-10627 JJ denotes correct
T26588 10628-10632 NN denotes size
T26590 10633-10637 VBD denotes were
T26585 10638-10645 VBN denotes excised
T26591 10646-10649 CC denotes and
T26592 10650-10659 VBN denotes incubated
T26593 10660-10662 IN denotes in
T26594 10663-10666 CD denotes 100
T26595 10667-10669 NN denotes μl
T26596 10670-10672 IN denotes of
T26597 10673-10680 JJ denotes sterile
T26598 10681-10684 NN denotes H2O
T26599 10685-10687 IN denotes at
T26600 10688-10690 CD denotes 80
T26601 10690-10692 NN denotes °C
T26602 10693-10696 IN denotes for
T26603 10697-10699 CD denotes 10
T26604 10700-10703 NN denotes min
T26605 10704-10706 TO denotes to
T26606 10707-10712 VB denotes elute
T26607 10713-10716 NN denotes DNA
T26608 10716-10717 . denotes .
T26609 10717-10892 sentence denotes Reamplification reactions consisted of 1X PCR buffer, 1.5 mM MgCl2, 0.17 mM dNTPs, 1 μM each primer, 0.1 unit of Taq (Promega) and 10 μl of eluate in a total volume of 50 μl.
T26610 10718-10733 NN denotes Reamplification
T26611 10734-10743 NNS denotes reactions
T26612 10744-10753 VBD denotes consisted
T26613 10754-10756 IN denotes of
T26614 10757-10759 NN denotes 1X
T26616 10760-10763 NN denotes PCR
T26615 10764-10770 NN denotes buffer
T26617 10770-10772 , denotes ,
T26618 10772-10775 CD denotes 1.5
T26619 10776-10778 NN denotes mM
T26620 10779-10784 NN denotes MgCl2
T26621 10784-10786 , denotes ,
T26622 10786-10790 CD denotes 0.17
T26623 10791-10793 NN denotes mM
T26624 10794-10799 NNS denotes dNTPs
T26625 10799-10801 , denotes ,
T26626 10801-10802 CD denotes 1
T26627 10803-10805 NN denotes μM
T26629 10806-10810 DT denotes each
T26628 10811-10817 NN denotes primer
T26630 10817-10819 , denotes ,
T26631 10819-10822 CD denotes 0.1
T26632 10823-10827 NN denotes unit
T26633 10828-10830 IN denotes of
T26634 10831-10834 NN denotes Taq
T26635 10835-10836 -LRB- denotes (
T26636 10836-10843 NNP denotes Promega
T26637 10843-10844 -RRB- denotes )
T26638 10845-10848 CC denotes and
T26639 10849-10851 CD denotes 10
T26640 10852-10854 NN denotes μl
T26641 10855-10857 IN denotes of
T26642 10858-10864 NN denotes eluate
T26643 10865-10867 IN denotes in
T26644 10868-10869 DT denotes a
T26646 10870-10875 JJ denotes total
T26645 10876-10882 NN denotes volume
T26647 10883-10885 IN denotes of
T26648 10886-10888 CD denotes 50
T26649 10889-10891 NN denotes μl
T26650 10891-10892 . denotes .
T26651 10892-10961 sentence denotes The same cycling parameters were used for reamplification reactions.
T26652 10893-10896 DT denotes The
T26654 10897-10901 JJ denotes same
T26655 10902-10909 NN denotes cycling
T26653 10910-10920 NNS denotes parameters
T26657 10921-10925 VBD denotes were
T26656 10926-10930 VBN denotes used
T26658 10931-10934 IN denotes for
T26659 10935-10950 NN denotes reamplification
T26660 10951-10960 NNS denotes reactions
T26661 10960-10961 . denotes .
T26662 10961-11104 sentence denotes Products were visualized on 1% 0.5X TBE agarose gels containing 0.06 μg/ml EtBr, excised and purfied using PCR purification columns (Promega).
T26663 10962-10970 NNS denotes Products
T26665 10971-10975 VBD denotes were
T26664 10976-10986 VBN denotes visualized
T26666 10987-10989 IN denotes on
T26667 10990-10991 CD denotes 1
T26668 10991-10992 NN denotes %
T26670 10993-10997 NN denotes 0.5X
T26671 10998-11001 NN denotes TBE
T26672 11002-11009 NN denotes agarose
T26669 11010-11014 NNS denotes gels
T26673 11015-11025 VBG denotes containing
T26674 11026-11030 CD denotes 0.06
T26675 11031-11033 NN denotes μg
T26677 11033-11034 SYM denotes /
T26678 11034-11036 NN denotes ml
T26676 11037-11041 NN denotes EtBr
T26679 11041-11043 , denotes ,
T26680 11043-11050 VBN denotes excised
T26681 11051-11054 CC denotes and
T26682 11055-11062 VBN denotes purfied
T26683 11063-11068 VBG denotes using
T26684 11069-11072 NN denotes PCR
T26685 11073-11085 NN denotes purification
T26686 11086-11093 NNS denotes columns
T26687 11094-11095 -LRB- denotes (
T26688 11095-11102 NNP denotes Promega
T26689 11102-11103 -RRB- denotes )
T26690 11103-11104 . denotes .
T26691 11104-11261 sentence denotes To quantity each fragment 1 μl of each purified product was run on a 1.0% agarose gel containing 0.06 μg/ml EtBr, along with a DNA mass ladder (Invitrogen).
T26692 11105-11107 TO denotes To
T26693 11108-11116 VB denotes quantity
T26695 11117-11121 DT denotes each
T26696 11122-11130 NN denotes fragment
T26697 11131-11132 CD denotes 1
T26698 11133-11135 NN denotes μl
T26699 11136-11138 IN denotes of
T26700 11139-11143 DT denotes each
T26702 11144-11152 VBN denotes purified
T26701 11153-11160 NN denotes product
T26703 11161-11164 VBD denotes was
T26694 11165-11168 VBN denotes run
T26704 11169-11171 IN denotes on
T26705 11172-11173 DT denotes a
T26707 11174-11177 CD denotes 1.0
T26708 11177-11178 NN denotes %
T26709 11179-11186 NN denotes agarose
T26706 11187-11190 NN denotes gel
T26710 11191-11201 VBG denotes containing
T26711 11202-11206 CD denotes 0.06
T26712 11207-11209 NN denotes μg
T26714 11209-11210 SYM denotes /
T26715 11210-11212 NN denotes ml
T26713 11213-11217 NN denotes EtBr
T26716 11217-11219 , denotes ,
T26717 11219-11224 IN denotes along
T26718 11225-11229 IN denotes with
T26719 11230-11231 DT denotes a
T26721 11232-11235 NN denotes DNA
T26722 11236-11240 NN denotes mass
T26720 11241-11247 NN denotes ladder
T26723 11248-11249 -LRB- denotes (
T26724 11249-11259 NNP denotes Invitrogen
T26725 11259-11260 -RRB- denotes )
T26726 11260-11261 . denotes .
T26727 11261-11351 sentence denotes In a 96 well plate, 15 ng of each PCR product was added to 5 pM of primer for sequencing.
T26728 11262-11264 IN denotes In
T26730 11265-11266 DT denotes a
T26732 11267-11269 CD denotes 96
T26733 11270-11274 NN denotes well
T26731 11275-11280 NN denotes plate
T26734 11280-11282 , denotes ,
T26735 11282-11284 CD denotes 15
T26736 11285-11287 NN denotes ng
T26737 11288-11290 IN denotes of
T26738 11291-11295 DT denotes each
T26740 11296-11299 NN denotes PCR
T26739 11300-11307 NN denotes product
T26741 11308-11311 VBD denotes was
T26729 11312-11317 VBN denotes added
T26742 11318-11320 IN denotes to
T26743 11321-11322 CD denotes 5
T26744 11323-11325 NN denotes pM
T26745 11326-11328 IN denotes of
T26746 11329-11335 NN denotes primer
T26747 11336-11339 IN denotes for
T26748 11340-11350 NN denotes sequencing
T26749 11350-11351 . denotes .
T26750 11351-11523 sentence denotes The College of Agriculture and Environmental Sciences (CAES) Genomics Facility at the University of California, Davis, performed bidirectional sequencing of each amplicon.
T26751 11352-11355 DT denotes The
T26752 11356-11363 NNP denotes College
T26754 11364-11366 IN denotes of
T26755 11367-11378 NNP denotes Agriculture
T26757 11379-11382 CC denotes and
T26758 11383-11396 NNP denotes Environmental
T26756 11397-11405 NNPS denotes Sciences
T26759 11406-11407 -LRB- denotes (
T26760 11407-11411 NNP denotes CAES
T26761 11411-11412 -RRB- denotes )
T26762 11413-11421 NNP denotes Genomics
T26753 11422-11430 NN denotes Facility
T26764 11431-11433 IN denotes at
T26765 11434-11437 DT denotes the
T26766 11438-11448 NNP denotes University
T26767 11449-11451 IN denotes of
T26768 11452-11462 NNP denotes California
T26769 11462-11464 , denotes ,
T26770 11464-11469 NNP denotes Davis
T26771 11469-11471 , denotes ,
T26763 11471-11480 VBD denotes performed
T26772 11481-11494 JJ denotes bidirectional
T26773 11495-11505 NN denotes sequencing
T26774 11506-11508 IN denotes of
T26775 11509-11513 DT denotes each
T26776 11514-11522 NN denotes amplicon
T26777 11522-11523 . denotes .
T26935 11525-11533 NN denotes Sequence
T26936 11534-11542 NN denotes analysis
T26937 11542-11657 sentence denotes B6 mRNA sequences for each gene were downloaded from the May 2004 (mm5) UCSC (NCBI Build 33) genome assembly [28].
T26938 11543-11545 NN denotes B6
T26940 11546-11550 NN denotes mRNA
T26939 11551-11560 NNS denotes sequences
T26942 11561-11564 IN denotes for
T26943 11565-11569 DT denotes each
T26944 11570-11574 NN denotes gene
T26945 11575-11579 VBD denotes were
T26941 11580-11590 VBN denotes downloaded
T26946 11591-11595 IN denotes from
T26947 11596-11599 DT denotes the
T26949 11600-11603 NNP denotes May
T26950 11604-11608 CD denotes 2004
T26951 11609-11610 -LRB- denotes (
T26952 11610-11613 NN denotes mm5
T26953 11613-11614 -RRB- denotes )
T26954 11615-11619 NNP denotes UCSC
T26955 11620-11621 -LRB- denotes (
T26957 11621-11625 NNP denotes NCBI
T26956 11626-11631 NNP denotes Build
T26958 11632-11634 CD denotes 33
T26959 11634-11635 -RRB- denotes )
T26960 11636-11642 NN denotes genome
T26948 11643-11651 NN denotes assembly
T26961 11652-11653 -LRB- denotes [
T26962 11653-11655 CD denotes 28
T26963 11655-11656 -RRB- denotes ]
T26964 11656-11657 . denotes .
T26965 11657-11809 sentence denotes These sequences were imported along with all CAST sequence traces into the SeqMan sequence assembly program (DNASTAR) and manually curated for quality.
T26966 11658-11663 DT denotes These
T26967 11664-11673 NNS denotes sequences
T26969 11674-11678 VBD denotes were
T26968 11679-11687 VBN denotes imported
T26970 11688-11693 IN denotes along
T26971 11694-11698 IN denotes with
T26972 11699-11702 DT denotes all
T26974 11703-11707 NN denotes CAST
T26975 11708-11716 NN denotes sequence
T26973 11717-11723 NNS denotes traces
T26976 11724-11728 IN denotes into
T26977 11729-11732 DT denotes the
T26979 11733-11739 NNP denotes SeqMan
T26980 11740-11748 NN denotes sequence
T26981 11749-11757 NN denotes assembly
T26978 11758-11765 NN denotes program
T26982 11766-11767 -LRB- denotes (
T26983 11767-11774 NNP denotes DNASTAR
T26984 11774-11775 -RRB- denotes )
T26985 11776-11779 CC denotes and
T26986 11780-11788 RB denotes manually
T26987 11789-11796 VBN denotes curated
T26988 11797-11800 IN denotes for
T26989 11801-11808 NN denotes quality
T26990 11808-11809 . denotes .
T26991 11809-11846 sentence denotes Poor quality reads were resequenced.
T26992 11810-11814 JJ denotes Poor
T26993 11815-11822 NN denotes quality
T26994 11823-11828 NNS denotes reads
T26996 11829-11833 VBD denotes were
T26995 11834-11845 VBN denotes resequenced
T26997 11845-11846 . denotes .
T26998 11846-11958 sentence denotes Individual contigs were created for each gene and polymorphisms were detected and curated by manual inspection.
T26999 11847-11857 JJ denotes Individual
T27000 11858-11865 NNS denotes contigs
T27002 11866-11870 VBD denotes were
T27001 11871-11878 VBN denotes created
T27003 11879-11882 IN denotes for
T27004 11883-11887 DT denotes each
T27005 11888-11892 NN denotes gene
T27006 11893-11896 CC denotes and
T27007 11897-11910 NNS denotes polymorphisms
T27009 11911-11915 VBD denotes were
T27008 11916-11924 VBN denotes detected
T27010 11925-11928 CC denotes and
T27011 11929-11936 VBN denotes curated
T27012 11937-11939 IN denotes by
T27013 11940-11946 JJ denotes manual
T27014 11947-11957 NN denotes inspection
T27015 11957-11958 . denotes .
R6266 T20228 T20229 compound Mouse,strains
R6267 T20230 T20229 cc and,strains
R6268 T20231 T20229 conj husbandry,strains
R6269 T20233 T20234 nmod CAST,EiJ
R6270 T20234 T20236 nmod EiJ,mice
R6271 T20235 T20234 punct /,EiJ
R6272 T20236 T20242 nsubjpass mice,purchased
R6273 T20237 T20238 punct (,#
R6274 T20238 T20236 parataxis #,mice
R6275 T20239 T20238 compound stock,#
R6276 T20240 T20238 nummod 000928,#
R6277 T20241 T20238 punct ),#
R6278 T20243 T20242 auxpass were,purchased
R6279 T20244 T20242 prep from,purchased
R6280 T20245 T20246 det the,Laboratory
R6281 T20246 T20244 pobj Laboratory,from
R6282 T20247 T20246 compound Jackson,Laboratory
R6283 T20248 T20242 punct .,purchased
R6284 T20250 T20251 det The,mutation
R6285 T20251 T20253 nsubjpass mutation,identified
R6286 T20252 T20251 compound hg,mutation
R6287 T20254 T20253 auxpass was,identified
R6288 T20255 T20253 advmod originally,identified
R6289 T20256 T20253 prep in,identified
R6290 T20257 T20258 det a,line
R6291 T20258 T20256 pobj line,in
R6292 T20259 T20258 amod selected,line
R6293 T20260 T20258 compound outcross,line
R6294 T20261 T20262 punct [,22
R6295 T20262 T20253 parataxis 22,identified
R6296 T20263 T20262 punct ],22
R6297 T20264 T20253 cc and,identified
R6298 T20265 T20266 aux has,introgressed
R6299 T20266 T20253 conj introgressed,identified
R6300 T20267 T20266 auxpass been,introgressed
R6301 T20268 T20266 prep onto,introgressed
R6302 T20269 T20270 det a,background
R6303 T20270 T20268 pobj background,onto
R6304 T20271 T20270 compound B6,background
R6305 T20272 T20266 prep via,introgressed
R6306 T20273 T20274 nummod nine,backcrosses
R6307 T20274 T20272 pobj backcrosses,via
R6308 T20275 T20276 aux to,create
R6309 T20276 T20266 advcl create,introgressed
R6310 T20277 T20278 det the,strain
R6311 T20278 T20276 dobj strain,create
R6312 T20279 T20278 compound HG,strain
R6313 T20280 T20253 punct .,identified
R6314 T20282 T20283 compound HG,mice
R6315 T20283 T20284 nsubj mice,were
R6316 T20285 T20283 acl used,mice
R6317 T20286 T20285 prep in,used
R6318 T20287 T20288 det this,experiment
R6319 T20288 T20286 pobj experiment,in
R6320 T20289 T20284 prep from,were
R6321 T20290 T20291 det the,generation
R6322 T20291 T20289 pobj generation,from
R6323 T20292 T20291 amod 17th,generation
R6324 T20293 T20292 cc or,17th
R6325 T20294 T20292 conj later,17th
R6326 T20295 T20291 prep of,generation
R6327 T20296 T20295 pobj inbreeding,of
R6328 T20297 T20284 punct .,were
R6329 T20299 T20300 nsubjpass Mice,housed
R6330 T20301 T20300 auxpass were,housed
R6331 T20302 T20300 prep in,housed
R6332 T20303 T20304 compound polycarbonate,cages
R6333 T20304 T20302 pobj cages,in
R6334 T20305 T20300 prep under,housed
R6335 T20306 T20307 amod controlled,conditions
R6336 T20307 T20305 pobj conditions,under
R6337 T20308 T20307 prep of,conditions
R6338 T20309 T20308 pobj temperature,of
R6339 T20310 T20311 punct (,°C
R6340 T20311 T20309 parataxis °C,temperature
R6341 T20312 T20311 nummod 21,°C
R6342 T20313 T20311 punct ±,°C
R6343 T20314 T20315 nummod 2,°C
R6344 T20315 T20311 appos °C,°C
R6345 T20316 T20311 punct ),°C
R6346 T20317 T20309 punct ", ",temperature
R6347 T20318 T20309 conj humidity,temperature
R6348 T20319 T20320 punct (,%
R6349 T20320 T20318 parataxis %,humidity
R6350 T20321 T20322 quantmod 40,70
R6351 T20322 T20320 nummod 70,%
R6352 T20323 T20322 punct –,70
R6353 T20324 T20320 punct ),%
R6354 T20325 T20318 cc and,humidity
R6355 T20326 T20318 conj lighting,humidity
R6356 T20327 T20328 punct (,dark
R6357 T20328 T20326 parataxis dark,lighting
R6358 T20329 T20330 nummod 14,h
R6359 T20330 T20331 compound h,light
R6360 T20331 T20328 dep light,dark
R6361 T20332 T20328 punct ", ",dark
R6362 T20333 T20334 nummod 10,h
R6363 T20334 T20328 compound h,dark
R6364 T20335 T20328 punct ", ",dark
R6365 T20336 T20337 nsubj lights,on
R6366 T20337 T20328 advmod on,dark
R6367 T20338 T20337 prep at,on
R6368 T20339 T20340 nummod 7,AM
R6369 T20340 T20338 pobj AM,at
R6370 T20341 T20328 punct ),dark
R6371 T20342 T20300 punct ", ",housed
R6372 T20343 T20300 cc and,housed
R6373 T20344 T20300 conj managed,housed
R6374 T20345 T20344 prep according,managed
R6375 T20346 T20345 prep to,according
R6376 T20347 T20348 det the,guidelines
R6377 T20348 T20346 pobj guidelines,to
R6378 T20349 T20348 prep of,guidelines
R6379 T20350 T20351 det the,Association
R6380 T20351 T20349 pobj Association,of
R6381 T20352 T20351 compound American,Association
R6382 T20353 T20351 prep for,Association
R6383 T20354 T20353 pobj Accreditation,for
R6384 T20355 T20354 prep of,Accreditation
R6385 T20356 T20357 compound Laboratory,Animal
R6386 T20357 T20358 compound Animal,Care
R6387 T20358 T20355 pobj Care,of
R6388 T20359 T20351 punct (,Association
R6389 T20360 T20351 appos AAALAC,Association
R6390 T20361 T20300 punct ),housed
R6391 T20362 T20300 punct .,housed
R6392 T20781 T20782 nsubjpass DNA,isolated
R6393 T20783 T20781 prep for,DNA
R6394 T20784 T20783 pobj genotyping,for
R6395 T20785 T20782 auxpass was,isolated
R6396 T20786 T20782 prep from,isolated
R6397 T20787 T20788 quantmod 1.0,2.0
R6398 T20788 T20790 nummod 2.0,mm
R6399 T20789 T20788 punct –,2.0
R6400 T20790 T20791 compound mm,clips
R6401 T20791 T20786 pobj clips,from
R6402 T20792 T20791 compound tail,clips
R6403 T20793 T20782 prep by,isolated
R6404 T20794 T20793 pcomp digesting,by
R6405 T20795 T20794 prep with,digesting
R6406 T20796 T20797 compound Proteinase,K
R6407 T20797 T20795 pobj K,with
R6408 T20798 T20799 punct (,Fisher
R6409 T20799 T20797 parataxis Fisher,K
R6410 T20800 T20799 punct ),Fisher
R6411 T20801 T20794 prep at,digesting
R6412 T20802 T20803 nummod 55,°C
R6413 T20803 T20801 pobj °C,at
R6414 T20804 T20794 prep in,digesting
R6415 T20805 T20806 det a,buffer
R6416 T20806 T20804 pobj buffer,in
R6417 T20807 T20806 acl composed,buffer
R6418 T20808 T20807 prep of,composed
R6419 T20809 T20810 nummod 0.45,%
R6420 T20810 T20811 compound %,NP40
R6421 T20811 T20808 pobj NP40,of
R6422 T20812 T20813 punct (,Sigma
R6423 T20813 T20811 parataxis Sigma,NP40
R6424 T20814 T20813 punct ),Sigma
R6425 T20815 T20811 punct ", ",NP40
R6426 T20816 T20817 nummod 0.45,%
R6427 T20817 T20818 compound %,Tween
R6428 T20818 T20811 conj Tween,NP40
R6429 T20819 T20818 nummod 20,Tween
R6430 T20820 T20821 punct (,Fisher
R6431 T20821 T20818 parataxis Fisher,Tween
R6432 T20822 T20821 punct ),Fisher
R6433 T20823 T20818 cc and,Tween
R6434 T20824 T20825 compound 1X,buffer
R6435 T20825 T20818 conj buffer,Tween
R6436 T20826 T20825 compound PCR,buffer
R6437 T20827 T20828 punct (,Promega
R6438 T20828 T20825 parataxis Promega,buffer
R6439 T20829 T20828 punct ),Promega
R6440 T20830 T20782 punct .,isolated
R6441 T20832 T20833 det The,product
R6442 T20833 T20834 nsubjpass product,diluted
R6443 T20835 T20833 prep of,product
R6444 T20836 T20837 det this,digestion
R6445 T20837 T20835 pobj digestion,of
R6446 T20838 T20834 auxpass was,diluted
R6447 T20839 T20840 punct (,1
R6448 T20840 T20834 parataxis 1,diluted
R6449 T20841 T20842 punct :,10
R6450 T20842 T20840 prep 10,1
R6451 T20843 T20840 punct ),1
R6452 T20844 T20834 prep in,diluted
R6453 T20845 T20846 amod sterile,H2O
R6454 T20846 T20844 pobj H2O,in
R6455 T20847 T20834 cc and,diluted
R6456 T20848 T20834 conj used,diluted
R6457 T20849 T20848 prep for,used
R6458 T20850 T20849 pobj genotyping,for
R6459 T20851 T20848 prep without,used
R6460 T20852 T20853 amod further,purification
R6461 T20853 T20851 pobj purification,without
R6462 T20854 T20834 punct .,diluted
R6463 T20856 T20857 compound Microsatellite,genotyping
R6464 T20857 T20858 nsubjpass genotyping,performed
R6465 T20859 T20858 auxpass was,performed
R6466 T20860 T20858 advcl using,performed
R6467 T20861 T20862 amod standard,protocols
R6468 T20862 T20860 dobj protocols,using
R6469 T20863 T20862 nmod PCR,protocols
R6470 T20864 T20863 cc and,PCR
R6471 T20865 T20866 compound gel,electrophoresis
R6472 T20866 T20863 conj electrophoresis,PCR
R6473 T20867 T20858 punct .,performed
R6474 T20869 T20870 compound Reaction,conditions
R6475 T20870 T20871 nsubjpass conditions,listed
R6476 T20872 T20870 prep for,conditions
R6477 T20873 T20874 det each,marker
R6478 T20874 T20872 pobj marker,for
R6479 T20875 T20871 auxpass are,listed
R6480 T20876 T20871 prep in,listed
R6481 T20877 T20878 amod Additional,1
R6482 T20878 T20876 pobj 1,in
R6483 T20879 T20878 nmod File,1
R6484 T20880 T20878 cc and,1
R6485 T20881 T20878 conj 2,1
R6486 T20882 T20871 punct .,listed
R6487 T20884 T20885 npadvmod MMU2,congenic
R6488 T20885 T20886 amod congenic,mice
R6489 T20886 T20887 nsubjpass mice,genotyped
R6490 T20888 T20887 auxpass were,genotyped
R6491 T20889 T20887 prep for,genotyped
R6492 T20890 T20889 pobj hg,for
R6493 T20891 T20887 advcl using,genotyped
R6494 T20892 T20893 det a,assay
R6495 T20893 T20891 dobj assay,using
R6496 T20894 T20895 nummod two,primer
R6497 T20895 T20893 compound primer,assay
R6498 T20896 T20893 compound genotyping,assay
R6499 T20897 T20887 punct .,genotyped
R6500 T20899 T20900 nummod One,set
R6501 T20900 T20902 nsubj set,spanned
R6502 T20901 T20900 compound primer,set
R6503 T20903 T20900 punct (,set
R6504 T20904 T20905 compound HG,F
R6505 T20905 T20900 appos F,set
R6506 T20906 T20905 punct -,F
R6507 T20907 T20905 punct ", ",F
R6508 T20908 T20905 appos ctcctgtctgggctgtgag,F
R6509 T20909 T20905 cc and,F
R6510 T20910 T20911 compound HG,R
R6511 T20911 T20905 conj R,F
R6512 T20912 T20911 punct -,R
R6513 T20913 T20911 punct ", ",R
R6514 T20914 T20911 appos caaaggcagaagtggggtaa,R
R6515 T20915 T20902 punct ),spanned
R6516 T20916 T20917 det the,deletion
R6517 T20917 T20902 dobj deletion,spanned
R6518 T20918 T20917 compound hg,deletion
R6519 T20919 T20902 advcl producing,spanned
R6520 T20920 T20921 det a,product
R6521 T20921 T20919 dobj product,producing
R6522 T20922 T20923 nummod 447,bp
R6523 T20923 T20921 compound bp,product
R6524 T20924 T20919 prep in,producing
R6525 T20925 T20926 nmod hg,hg
R6526 T20926 T20928 nmod hg,mice
R6527 T20927 T20926 punct /,hg
R6528 T20928 T20924 pobj mice,in
R6529 T20929 T20926 cc and,hg
R6530 T20930 T20931 punct +,hg
R6531 T20931 T20926 conj hg,hg
R6532 T20932 T20931 punct /,hg
R6533 T20933 T20902 punct .,spanned
R6534 T20935 T20936 det The,set
R6535 T20936 T20938 nsubj set,amplified
R6536 T20937 T20936 amod other,set
R6537 T20939 T20936 punct (,set
R6538 T20940 T20936 appos CRADD3a.F,set
R6539 T20941 T20940 punct ", ",CRADD3a.F
R6540 T20942 T20940 appos gtccatcagcattcctgaaa,CRADD3a.F
R6541 T20943 T20940 cc and,CRADD3a.F
R6542 T20944 T20940 conj CRADD3.R,CRADD3a.F
R6543 T20945 T20944 punct ", ",CRADD3.R
R6544 T20946 T20944 appos tgtccagcaacagcattgtc,CRADD3.R
R6545 T20947 T20938 punct ),amplified
R6546 T20948 T20949 det a,amplicon
R6547 T20949 T20938 dobj amplicon,amplified
R6548 T20950 T20951 nummod 232,bp
R6549 T20951 T20949 compound bp,amplicon
R6550 T20952 T20949 compound Cradd,amplicon
R6551 T20953 T20949 punct (,amplicon
R6552 T20954 T20949 acl located,amplicon
R6553 T20955 T20954 prep within,located
R6554 T20956 T20957 det the,deletion
R6555 T20957 T20955 pobj deletion,within
R6556 T20958 T20957 compound hg,deletion
R6557 T20959 T20938 punct ),amplified
R6558 T20960 T20938 prep in,amplified
R6559 T20961 T20962 punct +,+
R6560 T20962 T20964 punct +,mice
R6561 T20963 T20962 punct /,+
R6562 T20964 T20960 pobj mice,in
R6563 T20965 T20962 cc and,+
R6564 T20966 T20967 punct +,hg
R6565 T20967 T20962 conj hg,+
R6566 T20968 T20967 punct /,hg
R6567 T20969 T20970 punct [,54
R6568 T20970 T20938 parataxis 54,amplified
R6569 T20971 T20970 punct ],54
R6570 T20972 T20938 punct .,amplified
R6571 T20974 T20975 det The,temperature
R6572 T20975 T20978 nsubj temperature,was
R6573 T20976 T20977 npadvmod PCR,annealing
R6574 T20977 T20975 amod annealing,temperature
R6575 T20979 T20980 nummod 55,°C
R6576 T20980 T20978 attr °C,was
R6577 T20981 T20978 cc and,was
R6578 T20982 T20983 det the,concentration
R6579 T20983 T20985 nsubj concentration,was
R6580 T20984 T20983 compound MgCl2,concentration
R6581 T20985 T20978 conj was,was
R6582 T20986 T20987 nummod 1.5,mM
R6583 T20987 T20985 attr mM,was
R6584 T20988 T20985 punct .,was
R6585 T21608 T21607 prep of,Development
R6586 T21609 T21610 nmod B6.CAST,MMU2
R6587 T21610 T21613 nmod MMU2,strains
R6588 T21611 T21609 cc and,B6.CAST
R6589 T21612 T21609 conj HG.CAST,B6.CAST
R6590 T21613 T21608 pobj strains,of
R6591 T21614 T21615 npadvmod speed,congenic
R6592 T21615 T21613 amod congenic,strains
R6593 T21617 T21618 det All,strains
R6594 T21618 T21621 nsubjpass strains,developed
R6595 T21619 T21620 npadvmod speed,congenic
R6596 T21620 T21618 amod congenic,strains
R6597 T21622 T21621 auxpass were,developed
R6598 T21623 T21621 advcl starting,developed
R6599 T21624 T21623 prep with,starting
R6600 T21625 T21626 det an,cross
R6601 T21626 T21624 pobj cross,with
R6602 T21627 T21626 amod initial,cross
R6603 T21628 T21626 prep between,cross
R6604 T21629 T21630 det a,male
R6605 T21630 T21628 pobj male,between
R6606 T21631 T21630 compound CAST,male
R6607 T21632 T21630 cc and,male
R6608 T21633 T21634 compound HG,females
R6609 T21634 T21630 conj females,male
R6610 T21635 T21636 punct (,Figure
R6611 T21636 T21623 parataxis Figure,starting
R6612 T21637 T21636 nummod 1,Figure
R6613 T21638 T21636 punct ),Figure
R6614 T21639 T21640 punct [,6
R6615 T21640 T21621 parataxis 6,developed
R6616 T21641 T21640 nummod 5,6
R6617 T21642 T21640 punct ",",6
R6618 T21643 T21640 punct ],6
R6619 T21644 T21621 punct .,developed
R6620 T21646 T21647 amod Male,mice
R6621 T21647 T21649 nsubjpass mice,backcrossed
R6622 T21648 T21647 compound F1,mice
R6623 T21650 T21649 auxpass were,backcrossed
R6624 T21651 T21649 advmod then,backcrossed
R6625 T21652 T21649 prep to,backcrossed
R6626 T21653 T21654 compound HG,females
R6627 T21654 T21652 pobj females,to
R6628 T21655 T21649 punct .,backcrossed
R6629 T21657 T21658 det All,males
R6630 T21658 T21677 nsubjpass males,genotyped
R6631 T21659 T21658 nmod agouti,males
R6632 T21660 T21661 punct (,located
R6633 T21661 T21658 parataxis located,males
R6634 T21662 T21663 det the,locus
R6635 T21663 T21661 nsubjpass locus,located
R6636 T21664 T21663 amod dominant,locus
R6637 T21665 T21666 amod nonagouti,a
R6638 T21666 T21663 amod a,locus
R6639 T21667 T21666 punct (,a
R6640 T21668 T21663 punct ),locus
R6641 T21669 T21661 auxpass is,located
R6642 T21670 T21661 prep at,located
R6643 T21671 T21672 nummod 154.8,Mbp
R6644 T21672 T21670 pobj Mbp,at
R6645 T21673 T21661 prep on,located
R6646 T21674 T21673 pobj MMU2,on
R6647 T21675 T21661 punct ),located
R6648 T21676 T21658 compound N2,males
R6649 T21678 T21677 auxpass were,genotyped
R6650 T21679 T21677 prep for,genotyped
R6651 T21680 T21681 nummod 79,markers
R6652 T21681 T21679 pobj markers,for
R6653 T21682 T21681 compound microsatellite,markers
R6654 T21683 T21684 punct (,File
R6655 T21684 T21677 parataxis File,genotyped
R6656 T21685 T21684 amod Additional,File
R6657 T21686 T21684 nummod 1,File
R6658 T21687 T21684 punct ),File
R6659 T21688 T21677 punct .,genotyped
R6660 T21690 T21691 det These,markers
R6661 T21691 T21692 nsubjpass markers,spaced
R6662 T21693 T21692 auxpass were,spaced
R6663 T21694 T21692 advmod evenly,spaced
R6664 T21695 T21692 prep across,spaced
R6665 T21696 T21697 det the,genome
R6666 T21697 T21695 pobj genome,across
R6667 T21698 T21692 punct ", ",spaced
R6668 T21699 T21700 advmod except,in
R6669 T21700 T21692 prep in,spaced
R6670 T21701 T21700 pobj regions,in
R6671 T21702 T21703 advmod previously,identified
R6672 T21703 T21701 acl identified,regions
R6673 T21704 T21703 prep as,identified
R6674 T21705 T21704 pcomp harboring,as
R6675 T21706 T21705 dobj QTL,harboring
R6676 T21707 T21708 punct [,1
R6677 T21708 T21705 parataxis 1,harboring
R6678 T21709 T21708 punct ],1
R6679 T21710 T21701 punct ", ",regions
R6680 T21711 T21712 dep which,screened
R6681 T21712 T21701 relcl screened,regions
R6682 T21713 T21712 auxpass were,screened
R6683 T21714 T21715 advmod more,densely
R6684 T21715 T21712 advmod densely,screened
R6685 T21716 T21692 punct .,spaced
R6686 T21718 T21719 det The,male
R6687 T21719 T21725 nsubjpass male,selected
R6688 T21720 T21719 punct """",male
R6689 T21721 T21719 amod best,male
R6690 T21722 T21719 punct """",male
R6691 T21723 T21724 compound N2,agouti
R6692 T21724 T21719 compound agouti,male
R6693 T21726 T21719 prep with,male
R6694 T21727 T21728 det the,level
R6695 T21728 T21726 pobj level,with
R6696 T21729 T21728 amod lowest,level
R6697 T21730 T21728 prep of,level
R6698 T21731 T21732 npadvmod genome,wide
R6699 T21732 T21734 amod wide,heterozygosity
R6700 T21733 T21732 punct -,wide
R6701 T21734 T21730 pobj heterozygosity,of
R6702 T21735 T21734 amod unwanted,heterozygosity
R6703 T21736 T21737 mark while,maintaining
R6704 T21737 T21725 advcl maintaining,selected
R6705 T21738 T21739 compound CAST,alleles
R6706 T21739 T21737 dobj alleles,maintaining
R6707 T21740 T21739 prep for,alleles
R6708 T21741 T21742 det all,markers
R6709 T21742 T21740 pobj markers,for
R6710 T21743 T21742 compound MMU2,markers
R6711 T21744 T21725 auxpass was,selected
R6712 T21745 T21725 prep for,selected
R6713 T21746 T21745 pobj breeding,for
R6714 T21747 T21725 punct .,selected
R6715 T21749 T21750 det This,scheme
R6716 T21750 T21752 nsubjpass scheme,used
R6717 T21751 T21750 compound selection,scheme
R6718 T21753 T21752 auxpass was,used
R6719 T21754 T21752 prep at,used
R6720 T21755 T21756 det each,generation
R6721 T21756 T21754 pobj generation,at
R6722 T21757 T21758 mark until,identified
R6723 T21758 T21752 advcl identified,used
R6724 T21759 T21760 det a,male
R6725 T21760 T21758 nsubjpass male,identified
R6726 T21761 T21760 compound N4,male
R6727 T21762 T21758 auxpass was,identified
R6728 T21763 T21758 prep as,identified
R6729 T21764 T21765 amod homozygous,HG
R6730 T21765 T21763 pobj HG,as
R6731 T21766 T21765 prep for,HG
R6732 T21767 T21768 det all,markers
R6733 T21768 T21766 pobj markers,for
R6734 T21769 T21768 acl typed,markers
R6735 T21770 T21769 prep outside,typed
R6736 T21771 T21770 pobj MMU2,outside
R6737 T21772 T21752 punct .,used
R6738 T21774 T21775 prep After,identified
R6739 T21776 T21777 det an,backcross
R6740 T21777 T21774 pobj backcross,After
R6741 T21778 T21777 amod additional,backcross
R6742 T21779 T21777 prep to,backcross
R6743 T21780 T21781 compound HG,females
R6744 T21781 T21779 pobj females,to
R6745 T21782 T21775 punct ", ",identified
R6746 T21783 T21784 amod recombinant,males
R6747 T21784 T21775 nsubjpass males,identified
R6748 T21785 T21775 auxpass were,identified
R6749 T21786 T21775 advcl providing,identified
R6750 T21787 T21788 det the,foundation
R6751 T21788 T21786 dobj foundation,providing
R6752 T21789 T21788 prep for,foundation
R6753 T21790 T21791 det the,regions
R6754 T21791 T21789 pobj regions,for
R6755 T21792 T21791 nummod four,regions
R6756 T21793 T21791 amod overlapping,regions
R6757 T21794 T21791 compound donor,regions
R6758 T21795 T21775 punct .,identified
R6759 T21797 T21798 amod Selected,males
R6760 T21798 T21800 nsubjpass males,backcrossed
R6761 T21799 T21798 amod recombinant,males
R6762 T21801 T21800 auxpass were,backcrossed
R6763 T21802 T21800 advmod then,backcrossed
R6764 T21803 T21800 prep to,backcrossed
R6765 T21804 T21805 preconj both,B6
R6766 T21805 T21806 nmod B6,females
R6767 T21806 T21803 pobj females,to
R6768 T21807 T21805 cc and,B6
R6769 T21808 T21805 conj HG,B6
R6770 T21809 T21810 aux to,create
R6771 T21810 T21800 advcl create,backcrossed
R6772 T21811 T21810 dobj strains,create
R6773 T21812 T21811 punct ", ",strains
R6774 T21813 T21814 dep which,were
R6775 T21814 T21811 relcl were,strains
R6776 T21815 T21816 npadvmod B6,congenic
R6777 T21816 T21814 acomp congenic,were
R6778 T21817 T21818 punct (,+
R6779 T21818 T21815 punct +,B6
R6780 T21819 T21818 punct +,+
R6781 T21820 T21818 punct /,+
R6782 T21821 T21818 punct ),+
R6783 T21822 T21815 cc or,B6
R6784 T21823 T21815 conj HG,B6
R6785 T21824 T21825 punct (,hg
R6786 T21825 T21823 parataxis hg,HG
R6787 T21826 T21825 compound hg,hg
R6788 T21827 T21825 punct /,hg
R6789 T21828 T21825 punct ),hg
R6790 T21829 T21815 cc and,B6
R6791 T21830 T21815 conj heterozygous,B6
R6792 T21831 T21800 punct .,backcrossed
R6793 T21833 T21834 det These,mice
R6794 T21834 T21835 nsubjpass mice,intermated
R6795 T21836 T21835 auxpass were,intermated
R6796 T21837 T21838 aux to,produce
R6797 T21838 T21835 advcl produce,intermated
R6798 T21839 T21840 amod homozygous,founders
R6799 T21840 T21838 dobj founders,produce
R6800 T21841 T21838 prep for,produce
R6801 T21842 T21843 det each,strain
R6802 T21843 T21841 pobj strain,for
R6803 T21844 T21835 punct .,intermated
R6804 T21846 T21847 det This,scheme
R6805 T21847 T21850 nsubj scheme,created
R6806 T21848 T21847 amod novel,scheme
R6807 T21849 T21847 amod breeding,scheme
R6808 T21851 T21852 nummod four,congenics
R6809 T21852 T21850 dobj congenics,created
R6810 T21853 T21852 amod identical,congenics
R6811 T21854 T21852 compound founder,congenics
R6812 T21855 T21852 prep on,congenics
R6813 T21856 T21857 nummod two,backgrounds
R6814 T21857 T21855 pobj backgrounds,on
R6815 T21858 T21857 appos B6,backgrounds
R6816 T21859 T21860 punct (,+
R6817 T21860 T21858 punct +,B6
R6818 T21861 T21860 punct +,+
R6819 T21862 T21860 punct /,+
R6820 T21863 T21860 punct ),+
R6821 T21864 T21858 cc and,B6
R6822 T21865 T21858 conj HG,B6
R6823 T21866 T21867 punct (,hg
R6824 T21867 T21865 parataxis hg,HG
R6825 T21868 T21867 compound hg,hg
R6826 T21869 T21867 punct /,hg
R6827 T21870 T21867 punct ),hg
R6828 T21871 T21857 punct ", ",backgrounds
R6829 T21872 T21873 dep which,formed
R6830 T21873 T21857 relcl formed,backgrounds
R6831 T21874 T21875 det the,basis
R6832 T21875 T21873 dobj basis,formed
R6833 T21876 T21875 prep for,basis
R6834 T21877 T21878 poss our,examination
R6835 T21878 T21876 pobj examination,for
R6836 T21879 T21878 prep of,examination
R6837 T21880 T21879 pobj interactions,of
R6838 T21881 T21880 acl caused,interactions
R6839 T21882 T21881 agent by,caused
R6840 T21883 T21884 det the,presence
R6841 T21884 T21882 pobj presence,by
R6842 T21885 T21884 prep of,presence
R6843 T21886 T21887 det the,deletion
R6844 T21887 T21885 pobj deletion,of
R6845 T21888 T21887 compound hg,deletion
R6846 T21889 T21850 punct .,created
R6847 T21891 T21892 nmod MMU2,strains
R6848 T21892 T21895 nsubjpass strains,maintained
R6849 T21893 T21894 npadvmod speed,congenic
R6850 T21894 T21892 amod congenic,strains
R6851 T21896 T21895 auxpass were,maintained
R6852 T21897 T21895 prep through,maintained
R6853 T21898 T21899 compound brother,sister
R6854 T21899 T21901 compound sister,mating
R6855 T21900 T21899 punct -,sister
R6856 T21901 T21897 pobj mating,through
R6857 T21902 T21895 punct .,maintained
R6858 T21904 T21905 advmod Once,stabilized
R6859 T21905 T21909 advcl stabilized,used
R6860 T21906 T21907 det each,congenic
R6861 T21907 T21905 nsubjpass congenic,stabilized
R6862 T21908 T21905 auxpass was,stabilized
R6863 T21910 T21909 punct ", ",used
R6864 T21911 T21912 nummod 20,markers
R6865 T21912 T21909 nsubjpass markers,used
R6866 T21913 T21912 amod additional,markers
R6867 T21914 T21912 compound microsatellite,markers
R6868 T21915 T21909 auxpass were,used
R6869 T21916 T21917 aux to,refine
R6870 T21917 T21909 advcl refine,used
R6871 T21918 T21919 det the,position
R6872 T21919 T21917 dobj position,refine
R6873 T21920 T21919 prep of,position
R6874 T21921 T21922 det each,point
R6875 T21922 T21920 pobj point,of
R6876 T21923 T21922 amod congenic,point
R6877 T21924 T21922 amod recombinant,point
R6878 T21925 T21922 compound end,point
R6879 T21926 T21927 punct (,File
R6880 T21927 T21909 parataxis File,used
R6881 T21928 T21927 amod Additional,File
R6882 T21929 T21927 nummod 2,File
R6883 T21930 T21927 punct ),File
R6884 T21931 T21909 punct .,used
R6885 T22599 T22598 prep of,Development
R6886 T22600 T22601 nmod HG.CAST,strains
R6887 T22601 T22599 pobj strains,of
R6888 T22602 T22603 npadvmod speed,congenic
R6889 T22603 T22601 amod congenic,strains
R6890 T22604 T22601 prep for,strains
R6891 T22605 T22606 nmod MMU,1
R6892 T22606 T22604 pobj 1,for
R6893 T22607 T22606 punct ", ",1
R6894 T22608 T22606 conj 5,1
R6895 T22609 T22608 punct ", ",5
R6896 T22610 T22608 conj 8,5
R6897 T22611 T22610 punct ", ",8
R6898 T22612 T22610 conj 9,8
R6899 T22613 T22612 punct ", ",9
R6900 T22614 T22612 conj 11,9
R6901 T22615 T22614 cc and,11
R6902 T22616 T22614 conj 17,11
R6903 T22618 T22619 det All,males
R6904 T22619 T22622 nsubjpass males,genotyped
R6905 T22620 T22619 amod black,males
R6906 T22621 T22619 compound N2,males
R6907 T22623 T22619 prep from,males
R6908 T22624 T22625 det the,crosses
R6909 T22625 T22623 pobj crosses,from
R6910 T22626 T22625 amod first,crosses
R6911 T22627 T22625 nummod two,crosses
R6912 T22628 T22625 acl described,crosses
R6913 T22629 T22628 advmod above,described
R6914 T22630 T22622 auxpass were,genotyped
R6915 T22631 T22622 prep for,genotyped
R6916 T22632 T22633 nummod 12,markers
R6917 T22633 T22631 pobj markers,for
R6918 T22634 T22633 punct (,markers
R6919 T22635 T22633 appos D1Mit432,markers
R6920 T22636 T22635 punct ", ",D1Mit432
R6921 T22637 T22638 punct -,480
R6922 T22638 T22635 conj 480,D1Mit432
R6923 T22639 T22638 punct ", ",480
R6924 T22640 T22638 conj D5Mit353,480
R6925 T22641 T22640 punct ", ",D5Mit353
R6926 T22642 T22643 punct -,311
R6927 T22643 T22640 conj 311,D5Mit353
R6928 T22644 T22643 punct ", ",311
R6929 T22645 T22643 conj D9Mit60,311
R6930 T22646 T22645 punct ", ",D9Mit60
R6931 T22647 T22648 punct -,262
R6932 T22648 T22645 conj 262,D9Mit60
R6933 T22649 T22648 punct ", ",262
R6934 T22650 T22648 conj D11Mit5,262
R6935 T22651 T22650 punct ", ",D11Mit5
R6936 T22652 T22653 punct -,67
R6937 T22653 T22650 conj 67,D11Mit5
R6938 T22654 T22653 punct ", ",67
R6939 T22655 T22653 conj D8Mit234,67
R6940 T22656 T22655 punct ", ",D8Mit234
R6941 T22657 T22658 punct -,211
R6942 T22658 T22655 conj 211,D8Mit234
R6943 T22659 T22658 cc and,211
R6944 T22660 T22658 conj D17Mit28,211
R6945 T22661 T22660 cc and,D17Mit28
R6946 T22662 T22663 punct -,142
R6947 T22663 T22660 conj 142,D17Mit28
R6948 T22664 T22633 punct ),markers
R6949 T22665 T22633 punct ", ",markers
R6950 T22666 T22633 appos two,markers
R6951 T22667 T22666 acl spanning,two
R6952 T22668 T22667 dobj each,spanning
R6953 T22669 T22668 prep of,each
R6954 T22670 T22671 det the,regions
R6955 T22671 T22669 pobj regions,of
R6956 T22672 T22671 nummod six,regions
R6957 T22673 T22674 npadvmod QTL,harboring
R6958 T22674 T22671 amod harboring,regions
R6959 T22675 T22671 punct (,regions
R6960 T22676 T22677 nmod MMU,1
R6961 T22677 T22671 appos 1,regions
R6962 T22678 T22677 punct ", ",1
R6963 T22679 T22677 conj 5,1
R6964 T22680 T22679 punct ", ",5
R6965 T22681 T22679 conj 8,5
R6966 T22682 T22681 punct ", ",8
R6967 T22683 T22681 conj 9,8
R6968 T22684 T22683 punct ", ",9
R6969 T22685 T22683 conj 11,9
R6970 T22686 T22685 cc and,11
R6971 T22687 T22685 conj 17,11
R6972 T22688 T22689 punct ;,Table
R6973 T22689 T22622 parataxis Table,genotyped
R6974 T22690 T22689 nummod 1,Table
R6975 T22691 T22689 cc and,Table
R6976 T22692 T22693 amod Additional,File
R6977 T22693 T22689 conj File,Table
R6978 T22694 T22693 nummod 1,File
R6979 T22695 T22689 punct ),Table
R6980 T22696 T22622 punct .,genotyped
R6981 T22698 T22699 nsubjpass Markers,selected
R6982 T22700 T22699 auxpass were,selected
R6983 T22701 T22702 aux to,capture
R6984 T22702 T22699 advcl capture,selected
R6985 T22703 T22702 punct ", ",capture
R6986 T22704 T22702 prep at,capture
R6987 T22705 T22706 det a,minimum
R6988 T22706 T22704 pobj minimum,at
R6989 T22707 T22702 punct ", ",capture
R6990 T22708 T22709 det the,interval
R6991 T22709 T22702 dobj interval,capture
R6992 T22710 T22711 nummod 2,LOD
R6993 T22711 T22709 compound LOD,interval
R6994 T22712 T22711 punct -,LOD
R6995 T22713 T22709 compound support,interval
R6996 T22714 T22699 punct .,selected
R6997 T22716 T22717 nummod Two,males
R6998 T22717 T22719 nsubjpass males,selected
R6999 T22718 T22717 compound N2,males
R7000 T22720 T22719 auxpass were,selected
R7001 T22721 T22722 aux to,propagate
R7002 T22722 T22719 xcomp propagate,selected
R7003 T22723 T22724 det the,generation
R7004 T22724 T22722 dobj generation,propagate
R7005 T22725 T22724 compound N3,generation
R7006 T22726 T22719 punct ;,selected
R7007 T22727 T22719 dobj one,selected
R7008 T22728 T22727 amod heterozygous,one
R7009 T22729 T22728 prep for,heterozygous
R7010 T22730 T22729 pobj QTL,for
R7011 T22731 T22730 prep on,QTL
R7012 T22732 T22733 nmod MMU,1
R7013 T22733 T22731 pobj 1,on
R7014 T22734 T22733 cc and,1
R7015 T22735 T22733 conj 9,1
R7016 T22736 T22727 cc and,one
R7017 T22737 T22738 det the,other
R7018 T22738 T22727 conj other,one
R7019 T22739 T22738 amod heterozygous,other
R7020 T22740 T22739 prep for,heterozygous
R7021 T22741 T22740 pobj QTL,for
R7022 T22742 T22741 prep on,QTL
R7023 T22743 T22744 nmod MMU,5
R7024 T22744 T22742 pobj 5,on
R7025 T22745 T22744 punct ", ",5
R7026 T22746 T22744 conj 8,5
R7027 T22747 T22746 punct ", ",8
R7028 T22748 T22746 conj 11,8
R7029 T22749 T22748 cc and,11
R7030 T22750 T22748 conj 17,11
R7031 T22751 T22752 punct (,Figure
R7032 T22752 T22719 parataxis Figure,selected
R7033 T22753 T22752 nummod 1,Figure
R7034 T22754 T22752 punct ),Figure
R7035 T22755 T22719 punct .,selected
R7036 T22757 T22758 det Both,males
R7037 T22758 T22759 nsubj males,were
R7038 T22760 T22759 acomp homozygous,were
R7039 T22761 T22760 prep for,homozygous
R7040 T22762 T22763 compound HG,alleles
R7041 T22763 T22761 pobj alleles,for
R7042 T22764 T22759 prep at,were
R7043 T22765 T22766 det all,QTL
R7044 T22766 T22764 pobj QTL,at
R7045 T22767 T22766 amod other,QTL
R7046 T22768 T22766 amod known,QTL
R7047 T22769 T22759 punct .,were
R7048 T22771 T22772 det These,males
R7049 T22772 T22773 nsubjpass males,backcrossed
R7050 T22774 T22773 auxpass were,backcrossed
R7051 T22775 T22773 prep to,backcrossed
R7052 T22776 T22777 compound HG,females
R7053 T22777 T22775 pobj females,to
R7054 T22778 T22773 cc and,backcrossed
R7055 T22779 T22780 nsubjpass two,selected
R7056 T22780 T22773 conj selected,backcrossed
R7057 T22781 T22779 prep of,two
R7058 T22782 T22783 det the,males
R7059 T22783 T22781 pobj males,of
R7060 T22784 T22783 amod resulting,males
R7061 T22785 T22783 compound N3,males
R7062 T22786 T22783 acl inheriting,males
R7063 T22787 T22788 det the,sets
R7064 T22788 T22786 dobj sets,inheriting
R7065 T22789 T22788 amod same,sets
R7066 T22790 T22788 prep of,sets
R7067 T22791 T22790 pobj QTL,of
R7068 T22792 T22788 prep as,sets
R7069 T22793 T22794 poss their,sire
R7070 T22794 T22792 pobj sire,as
R7071 T22795 T22780 auxpass were,selected
R7072 T22796 T22780 prep for,selected
R7073 T22797 T22796 pobj breeding,for
R7074 T22798 T22780 punct .,selected
R7075 T22800 T22801 det These,males
R7076 T22801 T22802 nsubjpass males,backcrossed
R7077 T22803 T22802 auxpass were,backcrossed
R7078 T22804 T22802 advmod subsequently,backcrossed
R7079 T22805 T22802 prep to,backcrossed
R7080 T22806 T22807 compound HG,females
R7081 T22807 T22805 pobj females,to
R7082 T22808 T22802 cc and,backcrossed
R7083 T22809 T22810 nummod three,males
R7084 T22810 T22812 nsubjpass males,identified
R7085 T22811 T22810 compound N4,males
R7086 T22812 T22802 conj identified,backcrossed
R7087 T22813 T22812 auxpass were,identified
R7088 T22814 T22812 acomp heterozygous,identified
R7089 T22815 T22814 prep for,heterozygous
R7090 T22816 T22817 det the,regions
R7091 T22817 T22815 pobj regions,for
R7092 T22818 T22817 amod following,regions
R7093 T22819 T22817 punct : ,regions
R7094 T22820 T22821 meta 1,1
R7095 T22821 T22817 appos 1,regions
R7096 T22822 T22820 punct ),1
R7097 T22823 T22821 nmod MMU,1
R7098 T22824 T22821 cc and,1
R7099 T22825 T22821 conj 9,1
R7100 T22826 T22821 punct ;,1
R7101 T22827 T22828 meta 2,5
R7102 T22828 T22821 appos 5,1
R7103 T22829 T22827 punct ),2
R7104 T22830 T22828 nmod MMU,5
R7105 T22831 T22828 cc and,5
R7106 T22832 T22828 conj 11,5
R7107 T22833 T22821 punct ;,1
R7108 T22834 T22835 meta 3,8
R7109 T22835 T22821 appos 8,1
R7110 T22836 T22834 punct ),3
R7111 T22837 T22835 nmod MMU,8
R7112 T22838 T22835 punct ", ",8
R7113 T22839 T22835 conj 11,8
R7114 T22840 T22839 cc and,11
R7115 T22841 T22839 conj 17,11
R7116 T22842 T22843 punct (,Figure
R7117 T22843 T22812 parataxis Figure,identified
R7118 T22844 T22843 nummod 1,Figure
R7119 T22845 T22843 punct ),Figure
R7120 T22846 T22812 punct .,identified
R7121 T22848 T22849 advcl Starting,selected
R7122 T22850 T22848 prep at,Starting
R7123 T22851 T22850 pobj N4,at
R7124 T22852 T22848 cc and,Starting
R7125 T22853 T22848 conj continuing,Starting
R7126 T22854 T22853 prep through,continuing
R7127 T22855 T22854 pobj N6,through
R7128 T22856 T22849 punct ", ",selected
R7129 T22857 T22858 det the,male
R7130 T22858 T22849 nsubjpass male,selected
R7131 T22859 T22858 punct """",male
R7132 T22860 T22858 amod best,male
R7133 T22861 T22858 punct """",male
R7134 T22862 T22858 prep with,male
R7135 T22863 T22864 det the,percent
R7136 T22864 T22862 pobj percent,with
R7137 T22865 T22864 amod lowest,percent
R7138 T22866 T22864 prep of,percent
R7139 T22867 T22868 amod unwanted,alleles
R7140 T22868 T22866 pobj alleles,of
R7141 T22869 T22868 compound donor,alleles
R7142 T22870 T22849 auxpass was,selected
R7143 T22871 T22849 prep after,selected
R7144 T22872 T22871 pcomp performing,after
R7145 T22873 T22874 det a,scan
R7146 T22874 T22872 dobj scan,performing
R7147 T22875 T22874 compound genome,scan
R7148 T22876 T22872 advcl using,performing
R7149 T22877 T22878 det the,markers
R7150 T22878 T22876 dobj markers,using
R7151 T22879 T22878 amod remaining,markers
R7152 T22880 T22878 nummod 67,markers
R7153 T22881 T22882 npadvmod genome,wide
R7154 T22882 T22878 amod wide,markers
R7155 T22883 T22882 punct -,wide
R7156 T22884 T22885 punct (,markers
R7157 T22885 T22878 parataxis markers,markers
R7158 T22886 T22885 nummod 79,markers
R7159 T22887 T22885 amod total,markers
R7160 T22888 T22885 cc minus,markers
R7161 T22889 T22890 det the,markers
R7162 T22890 T22885 conj markers,markers
R7163 T22891 T22890 nummod 12,markers
R7164 T22892 T22890 acl genotyped,markers
R7165 T22893 T22892 prep in,genotyped
R7166 T22894 T22895 det the,backcrosses
R7167 T22895 T22893 pobj backcrosses,in
R7168 T22896 T22895 amod first,backcrosses
R7169 T22897 T22895 nummod two,backcrosses
R7170 T22898 T22895 acl spanning,backcrosses
R7171 T22899 T22900 det the,intervals
R7172 T22900 T22898 dobj intervals,spanning
R7173 T22901 T22900 amod know,intervals
R7174 T22902 T22900 compound QTL,intervals
R7175 T22903 T22885 punct ),markers
R7176 T22904 T22849 punct .,selected
R7177 T22906 T22907 prep At,created
R7178 T22908 T22906 pobj N5,At
R7179 T22909 T22910 det a,strain
R7180 T22910 T22907 nsubjpass strain,created
R7181 T22911 T22910 amod distinct,strain
R7182 T22912 T22907 auxpass was,created
R7183 T22913 T22907 prep for,created
R7184 T22914 T22913 pobj each,for
R7185 T22915 T22914 prep of,each
R7186 T22916 T22917 det the,regions
R7187 T22917 T22915 pobj regions,of
R7188 T22918 T22917 nummod six,regions
R7189 T22919 T22917 amod individual,regions
R7190 T22920 T22917 compound donor,regions
R7191 T22921 T22907 cc and,created
R7192 T22922 T22923 amod heterozygous,mice
R7193 T22923 T22924 nsubjpass mice,intermated
R7194 T22924 T22907 conj intermated,created
R7195 T22925 T22924 auxpass were,intermated
R7196 T22926 T22927 punct (,Figure
R7197 T22927 T22907 parataxis Figure,created
R7198 T22928 T22927 nummod 1,Figure
R7199 T22929 T22927 punct ),Figure
R7200 T22930 T22907 punct .,created
R7201 T22932 T22933 amod Homozygous,strains
R7202 T22933 T22937 nsubjpass strains,maintained
R7203 T22934 T22933 nmod HG.CAST,strains
R7204 T22935 T22936 npadvmod speed,congenic
R7205 T22936 T22933 amod congenic,strains
R7206 T22938 T22937 auxpass were,maintained
R7207 T22939 T22937 prep through,maintained
R7208 T22940 T22941 compound brother,sister
R7209 T22941 T22943 compound sister,mating
R7210 T22942 T22941 punct -,sister
R7211 T22943 T22939 pobj mating,through
R7212 T22944 T22937 punct .,maintained
R7213 T22946 T22947 mark Once,stabilized
R7214 T22947 T22951 advcl stabilized,used
R7215 T22948 T22949 det each,congenic
R7216 T22949 T22947 nsubjpass congenic,stabilized
R7217 T22950 T22947 auxpass was,stabilized
R7218 T22952 T22951 punct ", ",used
R7219 T22953 T22954 nummod 19,markers
R7220 T22954 T22951 nsubjpass markers,used
R7221 T22955 T22954 amod additional,markers
R7222 T22956 T22954 compound microsatellite,markers
R7223 T22957 T22951 auxpass were,used
R7224 T22958 T22959 aux to,refine
R7225 T22959 T22951 advcl refine,used
R7226 T22960 T22961 det the,position
R7227 T22961 T22959 dobj position,refine
R7228 T22962 T22961 prep of,position
R7229 T22963 T22964 det each,point
R7230 T22964 T22962 pobj point,of
R7231 T22965 T22964 amod congenic,point
R7232 T22966 T22964 amod recombinant,point
R7233 T22967 T22964 compound end,point
R7234 T22968 T22969 punct (,File
R7235 T22969 T22951 parataxis File,used
R7236 T22970 T22969 amod Additional,File
R7237 T22971 T22969 nummod 2,File
R7238 T22972 T22969 punct ),File
R7239 T22973 T22951 punct .,used
R7240 T23421 T23420 prep of,Development
R7241 T23422 T23423 nmod B6.CASTC,strains
R7242 T23423 T23421 pobj strains,of
R7243 T23424 T23422 cc and,B6.CASTC
R7244 T23425 T23422 conj HG.CASTC,B6.CASTC
R7245 T23426 T23423 compound control,strains
R7246 T23428 T23429 nsubj HG,is
R7247 T23429 T23430 ccomp is,be
R7248 T23431 T23432 det a,strain
R7249 T23432 T23429 attr strain,is
R7250 T23433 T23434 prep in,introgressed
R7251 T23434 T23432 relcl introgressed,strain
R7252 T23435 T23433 pobj which,in
R7253 T23436 T23437 det the,deletion
R7254 T23437 T23434 nsubjpass deletion,introgressed
R7255 T23438 T23437 compound hg,deletion
R7256 T23439 T23434 aux has,introgressed
R7257 T23440 T23434 auxpass been,introgressed
R7258 T23441 T23434 prep onto,introgressed
R7259 T23442 T23443 det a,background
R7260 T23443 T23441 pobj background,onto
R7261 T23444 T23443 compound B6,background
R7262 T23445 T23430 punct ", ",be
R7263 T23446 T23430 advmod therefore,be
R7264 T23447 T23448 det the,differences
R7265 T23448 T23430 nsubj differences,be
R7266 T23449 T23448 amod only,differences
R7267 T23450 T23448 amod genetic,differences
R7268 T23451 T23448 prep between,differences
R7269 T23452 T23453 det the,strains
R7270 T23453 T23451 pobj strains,between
R7271 T23454 T23430 aux would,be
R7272 T23455 T23456 det the,locus
R7273 T23456 T23430 attr locus,be
R7274 T23457 T23456 compound hg,locus
R7275 T23458 T23456 punct ", ",locus
R7276 T23459 T23460 advmod tightly,linked
R7277 T23460 T23461 amod linked,alleles
R7278 T23461 T23456 conj alleles,locus
R7279 T23462 T23461 prep from,alleles
R7280 T23463 T23464 det the,strain
R7281 T23464 T23462 pobj strain,from
R7282 T23465 T23464 amod outbred,strain
R7283 T23466 T23467 prep on,arose
R7284 T23467 T23464 relcl arose,strain
R7285 T23468 T23466 pobj which,on
R7286 T23469 T23467 nsubj hg,arose
R7287 T23470 T23461 cc and,alleles
R7288 T23471 T23472 amod contaminating,alleles
R7289 T23472 T23461 conj alleles,alleles
R7290 T23473 T23472 acl remaining,alleles
R7291 T23474 T23473 prep after,remaining
R7292 T23475 T23476 det the,backcrosses
R7293 T23476 T23474 pobj backcrosses,after
R7294 T23477 T23476 nummod nine,backcrosses
R7295 T23478 T23473 cc and,remaining
R7296 T23479 T23473 conj fixed,remaining
R7297 T23480 T23479 prep during,fixed
R7298 T23481 T23480 pobj inbreeding,during
R7299 T23482 T23430 punct .,be
R7300 T23484 T23485 advmod Instead,of
R7301 T23485 T23486 prep of,choose
R7302 T23487 T23485 pcomp using,of
R7303 T23488 T23489 amod parental,strains
R7304 T23489 T23487 dobj strains,using
R7305 T23490 T23489 nmod B6,strains
R7306 T23491 T23490 cc and,B6
R7307 T23492 T23490 conj HG,B6
R7308 T23493 T23487 prep as,using
R7309 T23494 T23493 pobj controls,as
R7310 T23495 T23494 prep for,controls
R7311 T23496 T23497 amod phenotypic,comparisons
R7312 T23497 T23495 pobj comparisons,for
R7313 T23498 T23497 prep with,comparisons
R7314 T23499 T23500 det each,speed
R7315 T23500 T23498 pobj speed,with
R7316 T23501 T23500 amod congenic,speed
R7317 T23502 T23486 punct ", ",choose
R7318 T23503 T23486 nsubj we,choose
R7319 T23504 T23505 aux to,develop
R7320 T23505 T23486 xcomp develop,choose
R7321 T23506 T23507 amod independent,strains
R7322 T23507 T23505 dobj strains,develop
R7323 T23508 T23507 compound control,strains
R7324 T23509 T23507 acl originating,strains
R7325 T23510 T23509 prep from,originating
R7326 T23511 T23512 det the,cross
R7327 T23512 T23510 pobj cross,from
R7328 T23513 T23512 amod same,cross
R7329 T23514 T23512 prep as,cross
R7330 T23515 T23516 det the,panels
R7331 T23516 T23514 pobj panels,as
R7332 T23517 T23516 amod congenic,panels
R7333 T23518 T23486 punct .,choose
R7334 T23520 T23521 amod Separate,strains
R7335 T23521 T23533 nsubjpass strains,developed
R7336 T23522 T23523 nmod B6.CAST,control
R7337 T23523 T23521 nmod control,strains
R7338 T23524 T23523 punct (,control
R7339 T23525 T23523 appos B6C,control
R7340 T23526 T23523 punct ),control
R7341 T23527 T23523 cc and,control
R7342 T23528 T23529 compound HG.CAST,control
R7343 T23529 T23523 conj control,control
R7344 T23530 T23529 punct (,control
R7345 T23531 T23529 appos HGC,control
R7346 T23532 T23521 punct ),strains
R7347 T23534 T23533 auxpass were,developed
R7348 T23535 T23533 advcl using,developed
R7349 T23536 T23535 dobj mice,using
R7350 T23537 T23536 prep from,mice
R7351 T23538 T23539 det the,experiment
R7352 T23539 T23537 pobj experiment,from
R7353 T23540 T23539 compound MMU2,experiment
R7354 T23541 T23533 punct .,developed
R7355 T23543 T23544 nsubjpass Mice,intermated
R7356 T23545 T23543 prep from,Mice
R7357 T23546 T23547 det the,backcross
R7358 T23547 T23545 pobj backcross,from
R7359 T23548 T23547 amod last,backcross
R7360 T23549 T23543 acl inheriting,Mice
R7361 T23550 T23551 advmod only,alleles
R7362 T23551 T23549 dobj alleles,inheriting
R7363 T23552 T23551 nmod B6,alleles
R7364 T23553 T23552 cc or,B6
R7365 T23554 T23552 conj HG,B6
R7366 T23555 T23551 compound MMU2,alleles
R7367 T23556 T23549 prep at,inheriting
R7368 T23557 T23556 pobj markers,at
R7369 T23558 T23557 acl spanning,markers
R7370 T23559 T23558 dobj MMU2,spanning
R7371 T23560 T23544 auxpass were,intermated
R7372 T23561 T23562 aux to,serve
R7373 T23562 T23544 advcl serve,intermated
R7374 T23563 T23562 prep as,serve
R7375 T23564 T23565 det the,basis
R7376 T23565 T23563 pobj basis,as
R7377 T23566 T23565 prep for,basis
R7378 T23567 T23568 det each,control
R7379 T23568 T23566 pobj control,for
R7380 T23569 T23544 punct .,intermated
R7381 T23571 T23572 det Both,strains
R7382 T23572 T23574 nsubjpass strains,maintained
R7383 T23573 T23572 compound control,strains
R7384 T23575 T23574 auxpass were,maintained
R7385 T23576 T23574 advmod subsequently,maintained
R7386 T23577 T23574 prep through,maintained
R7387 T23578 T23579 compound brother,sister
R7388 T23579 T23581 compound sister,mating
R7389 T23580 T23579 punct -,sister
R7390 T23581 T23577 pobj mating,through
R7391 T23582 T23574 punct .,maintained
R7392 T23584 T23585 det The,strains
R7393 T23585 T23587 nsubj strains,were
R7394 T23586 T23585 compound control,strains
R7395 T23588 T23587 acomp coisogenic,were
R7396 T23589 T23588 prep with,coisogenic
R7397 T23590 T23591 det the,strain
R7398 T23591 T23589 pobj strain,with
R7399 T23592 T23591 amod parental,strain
R7400 T23593 T23591 nmod B6,strain
R7401 T23594 T23593 cc or,B6
R7402 T23595 T23593 conj HG,B6
R7403 T23596 T23587 prep with,were
R7404 T23597 T23598 det the,exception
R7405 T23598 T23596 pobj exception,with
R7406 T23599 T23598 prep of,exception
R7407 T23600 T23599 pobj mutations,of
R7408 T23601 T23602 dep that,arose
R7409 T23602 T23600 relcl arose,mutations
R7410 T23603 T23602 prep during,arose
R7411 T23604 T23605 amod congenic,construction
R7412 T23605 T23603 pobj construction,during
R7413 T23606 T23600 cc and,mutations
R7414 T23607 T23608 det a,percentage
R7415 T23608 T23600 conj percentage,mutations
R7416 T23609 T23608 amod small,percentage
R7417 T23610 T23608 prep of,percentage
R7418 T23611 T23612 amod contaminating,alleles
R7419 T23612 T23610 pobj alleles,of
R7420 T23613 T23612 compound donor,alleles
R7421 T23614 T23612 acl missed,alleles
R7422 T23615 T23614 prep after,missed
R7423 T23616 T23617 nummod 6,backcrosses
R7424 T23617 T23615 pobj backcrosses,after
R7425 T23618 T23587 punct .,were
R7426 T23620 T23621 advmod Therefore,are
R7427 T23622 T23621 punct ", ",are
R7428 T23623 T23624 mark since,developed
R7429 T23624 T23621 advcl developed,are
R7430 T23625 T23626 det the,congenics
R7431 T23626 T23624 nsubjpass congenics,developed
R7432 T23627 T23626 cc and,congenics
R7433 T23628 T23626 conj controls,congenics
R7434 T23629 T23624 auxpass were,developed
R7435 T23630 T23624 prep through,developed
R7436 T23631 T23632 det the,scheme
R7437 T23632 T23630 pobj scheme,through
R7438 T23633 T23632 amod same,scheme
R7439 T23634 T23632 compound selection,scheme
R7440 T23635 T23624 cc and,developed
R7441 T23636 T23637 advmod possibly,share
R7442 T23637 T23624 conj share,developed
R7443 T23638 T23639 amod common,regions
R7444 T23639 T23637 dobj regions,share
R7445 T23640 T23639 amod contaminating,regions
R7446 T23641 T23621 punct ", ",are
R7447 T23642 T23643 det the,strains
R7448 T23643 T23621 nsubj strains,are
R7449 T23644 T23643 nmod B6C,strains
R7450 T23645 T23644 cc and,B6C
R7451 T23646 T23644 conj HGC,B6C
R7452 T23647 T23648 det the,control
R7453 T23648 T23621 attr control,are
R7454 T23649 T23648 advmod most,control
R7455 T23650 T23648 amod ideal,control
R7456 T23651 T23652 aux to,compare
R7457 T23652 T23648 advcl compare,control
R7458 T23653 T23654 det each,congenic
R7459 T23654 T23652 dobj congenic,compare
R7460 T23655 T23621 punct .,are
R7463 T24427 T24428 amod Phenotypic,characterization
R7464 T24430 T24431 compound Trait,data
R7465 T24431 T24432 nsubjpass data,collected
R7466 T24433 T24432 auxpass were,collected
R7467 T24434 T24432 prep on,collected
R7468 T24435 T24436 advmod approximately,40
R7469 T24436 T24437 nummod 40,mice
R7470 T24437 T24434 pobj mice,on
R7471 T24438 T24439 punct (,20
R7472 T24439 T24437 parataxis 20,mice
R7473 T24440 T24439 prep of,20
R7474 T24441 T24442 det each,sex
R7475 T24442 T24440 pobj sex,of
R7476 T24443 T24439 punct ),20
R7477 T24444 T24437 prep from,mice
R7478 T24445 T24446 det each,strain
R7479 T24446 T24444 pobj strain,from
R7480 T24447 T24446 amod congenic,strain
R7481 T24448 T24447 cc and,congenic
R7482 T24449 T24447 conj control,congenic
R7483 T24450 T24432 punct .,collected
R7484 T24452 T24453 aux To,eliminate
R7485 T24453 T24454 advcl eliminate,characterized
R7486 T24455 T24453 dobj parity,eliminate
R7487 T24456 T24453 cc and,eliminate
R7488 T24457 T24453 conj reduce,eliminate
R7489 T24458 T24459 compound litter,size
R7490 T24459 T24460 compound size,effects
R7491 T24460 T24457 dobj effects,reduce
R7492 T24461 T24462 advmod only,progeny
R7493 T24462 T24454 nsubjpass progeny,characterized
R7494 T24463 T24462 prep from,progeny
R7495 T24464 T24465 amod uniparous,dams
R7496 T24465 T24463 pobj dams,from
R7497 T24466 T24454 auxpass were,characterized
R7498 T24467 T24454 cc and,characterized
R7499 T24468 T24469 det all,litters
R7500 T24469 T24470 nsubjpass litters,standardized
R7501 T24470 T24454 conj standardized,characterized
R7502 T24471 T24470 auxpass were,standardized
R7503 T24472 T24470 prep to,standardized
R7504 T24473 T24474 quantmod 5,7
R7505 T24474 T24476 nummod 7,pups
R7506 T24475 T24474 punct –,7
R7507 T24476 T24472 pobj pups,to
R7508 T24477 T24478 punct /,litter
R7509 T24478 T24476 prep litter,pups
R7510 T24479 T24470 punct .,standardized
R7511 T24481 T24482 nsubjpass Mice,weaned
R7512 T24483 T24482 auxpass were,weaned
R7513 T24484 T24482 prep at,weaned
R7514 T24485 T24486 nummod 3,weeks
R7515 T24486 T24484 pobj weeks,at
R7516 T24487 T24486 prep of,weeks
R7517 T24488 T24487 pobj age,of
R7518 T24489 T24482 punct .,weaned
R7519 T24491 T24492 nsubjpass Feed,offered
R7520 T24493 T24494 punct (,protein
R7521 T24494 T24491 parataxis protein,Feed
R7522 T24495 T24494 dep Purina,protein
R7523 T24496 T24495 nummod 5008,Purina
R7524 T24497 T24494 punct ;,protein
R7525 T24498 T24499 nummod 23.5,%
R7526 T24499 T24494 compound %,protein
R7527 T24500 T24494 punct ", ",protein
R7528 T24501 T24502 nummod 6.5,%
R7529 T24502 T24503 compound %,fat
R7530 T24503 T24494 appos fat,protein
R7531 T24504 T24494 punct ", ",protein
R7532 T24505 T24506 nummod 3.3,Kcal
R7533 T24506 T24494 appos Kcal,protein
R7534 T24507 T24508 punct /,g
R7535 T24508 T24506 prep g,Kcal
R7536 T24509 T24494 punct ),protein
R7537 T24510 T24491 cc and,Feed
R7538 T24511 T24491 conj water,Feed
R7539 T24512 T24492 auxpass were,offered
R7540 T24513 T24514 advmod ad,libitum
R7541 T24514 T24492 advmod libitum,offered
R7542 T24515 T24492 punct .,offered
R7543 T24517 T24518 nsubjpass Mice,weighed
R7544 T24519 T24518 auxpass were,weighed
R7545 T24520 T24518 prep to,weighed
R7546 T24521 T24522 det the,g
R7547 T24522 T24520 pobj g,to
R7548 T24523 T24522 amod nearest,g
R7549 T24524 T24522 nummod 0.1,g
R7550 T24525 T24518 prep at,weighed
R7551 T24526 T24525 pobj 2WK,at
R7552 T24527 T24526 punct ", ",2WK
R7553 T24528 T24526 conj 3WK,2WK
R7554 T24529 T24528 punct ", ",3WK
R7555 T24530 T24528 conj 6WK,3WK
R7556 T24531 T24530 punct ", ",6WK
R7557 T24532 T24530 cc and,6WK
R7558 T24533 T24530 conj 9WK,6WK
R7559 T24534 T24526 prep of,2WK
R7560 T24535 T24534 pobj age,of
R7561 T24536 T24518 punct .,weighed
R7562 T24538 T24539 prep At,anesthetized
R7563 T24540 T24538 pobj 9WK,At
R7564 T24541 T24540 punct (,9WK
R7565 T24542 T24540 punct ±,9WK
R7566 T24543 T24544 nummod 5,days
R7567 T24544 T24540 appos days,9WK
R7568 T24545 T24540 punct ),9WK
R7569 T24546 T24539 nsubjpass mice,anesthetized
R7570 T24547 T24539 auxpass were,anesthetized
R7571 T24548 T24539 prep under,anesthetized
R7572 T24549 T24548 pobj isoflurane,under
R7573 T24550 T24539 cc and,anesthetized
R7574 T24551 T24552 amod nasal,anal
R7575 T24552 T24554 amod anal,length
R7576 T24553 T24552 punct -,anal
R7577 T24554 T24555 nsubjpass length,measured
R7578 T24555 T24539 conj measured,anesthetized
R7579 T24556 T24554 punct (,length
R7580 T24557 T24554 appos NA,length
R7581 T24558 T24554 punct ),length
R7582 T24559 T24554 cc and,length
R7583 T24560 T24561 amod nasal,tail
R7584 T24561 T24554 conj tail,length
R7585 T24562 T24561 punct -,tail
R7586 T24563 T24561 punct (,tail
R7587 T24564 T24561 appos NT,tail
R7588 T24565 T24555 punct ),measured
R7589 T24566 T24555 auxpass were,measured
R7590 T24567 T24555 prep to,measured
R7591 T24568 T24569 det the,mm
R7592 T24569 T24567 pobj mm,to
R7593 T24570 T24569 amod nearest,mm
R7594 T24571 T24539 punct .,anesthetized
R7595 T24573 T24574 compound Tail,length
R7596 T24574 T24575 nsubjpass length,calculated
R7597 T24576 T24575 auxpass was,calculated
R7598 T24577 T24575 prep as,calculated
R7599 T24578 T24577 pobj NA,as
R7600 T24579 T24578 cc minus,NA
R7601 T24580 T24578 conj NT,NA
R7602 T24581 T24575 punct .,calculated
R7603 T24583 T24584 amod Anesthetized,mice
R7604 T24584 T24585 nsubjpass mice,sacrificed
R7605 T24586 T24585 auxpass were,sacrificed
R7606 T24587 T24585 advmod then,sacrificed
R7607 T24588 T24585 prep by,sacrificed
R7608 T24589 T24588 pobj decapitation,by
R7609 T24590 T24585 cc and,sacrificed
R7610 T24591 T24585 conj exsanguinated,sacrificed
R7611 T24592 T24585 punct .,sacrificed
R7612 T24594 T24595 amod Femoral,pad
R7613 T24595 T24597 nsubjpass pad,removed
R7614 T24596 T24595 compound fat,pad
R7615 T24598 T24595 punct (,pad
R7616 T24599 T24595 appos FFP,pad
R7617 T24600 T24595 punct ),pad
R7618 T24601 T24595 punct ", ",pad
R7619 T24602 T24603 amod gonadal,pad
R7620 T24603 T24595 conj pad,pad
R7621 T24604 T24603 compound fat,pad
R7622 T24605 T24603 punct (,pad
R7623 T24606 T24603 appos GFP,pad
R7624 T24607 T24603 punct ),pad
R7625 T24608 T24603 punct ", ",pad
R7626 T24609 T24610 amod mesenteric,pad
R7627 T24610 T24603 conj pad,pad
R7628 T24611 T24610 compound fat,pad
R7629 T24612 T24610 punct (,pad
R7630 T24613 T24610 appos MFP,pad
R7631 T24614 T24610 punct ),pad
R7632 T24615 T24610 cc and,pad
R7633 T24616 T24617 amod retroperitoneal,pad
R7634 T24617 T24610 conj pad,pad
R7635 T24618 T24617 compound fat,pad
R7636 T24619 T24617 punct (,pad
R7637 T24620 T24617 appos RFP,pad
R7638 T24621 T24597 punct ),removed
R7639 T24622 T24597 auxpass were,removed
R7640 T24623 T24597 cc and,removed
R7641 T24624 T24597 conj weighed,removed
R7642 T24625 T24624 prep to,weighed
R7643 T24626 T24627 det the,mg
R7644 T24627 T24625 pobj mg,to
R7645 T24628 T24627 amod nearest,mg
R7646 T24629 T24597 punct .,removed
R7647 T24631 T24632 amod Chemical,analysis
R7648 T24632 T24634 nsubjpass analysis,performed
R7649 T24633 T24632 amod compositional,analysis
R7650 T24635 T24634 auxpass was,performed
R7651 T24636 T24634 prep for,performed
R7652 T24637 T24638 nmod HGC,carcasses
R7653 T24638 T24636 pobj carcasses,for
R7654 T24639 T24637 punct ", ",HGC
R7655 T24640 T24637 conj HG11,HGC
R7656 T24641 T24640 cc and,HG11
R7657 T24642 T24640 conj HG17,HG11
R7658 T24643 T24644 mark as,described
R7659 T24644 T24634 advcl described,performed
R7660 T24645 T24644 advmod previously,described
R7661 T24646 T24634 prep with,performed
R7662 T24647 T24648 amod slight,modifications
R7663 T24648 T24646 pobj modifications,with
R7664 T24649 T24650 punct [,24
R7665 T24650 T24634 parataxis 24,performed
R7666 T24651 T24650 punct ],24
R7667 T24652 T24634 punct .,performed
R7668 T24654 T24655 advmod Briefly,returned
R7669 T24656 T24655 punct ", ",returned
R7670 T24657 T24655 prep after,returned
R7671 T24658 T24657 pobj weighing,after
R7672 T24659 T24660 det each,pad
R7673 T24660 T24655 nsubjpass pad,returned
R7674 T24661 T24660 compound fat,pad
R7675 T24662 T24655 auxpass was,returned
R7676 T24663 T24655 prep to,returned
R7677 T24664 T24665 det the,carcass
R7678 T24665 T24663 pobj carcass,to
R7679 T24666 T24655 punct .,returned
R7680 T24668 T24669 det The,tract
R7681 T24669 T24675 nsubjpass tract,removed
R7682 T24670 T24669 amod entire,tract
R7683 T24671 T24669 amod gastrointestinal,tract
R7684 T24672 T24673 punct (,GI
R7685 T24673 T24669 parataxis GI,tract
R7686 T24674 T24673 punct ),GI
R7687 T24676 T24675 auxpass was,removed
R7688 T24677 T24675 advmod subsequently,removed
R7689 T24678 T24675 cc and,removed
R7690 T24679 T24680 nsubjpass carcasses,weighed
R7691 T24680 T24675 conj weighed,removed
R7692 T24681 T24680 auxpass were,weighed
R7693 T24682 T24680 advmod again,weighed
R7694 T24683 T24680 punct .,weighed
R7695 T24685 T24686 nsubj This,represented
R7696 T24687 T24688 det the,weight
R7697 T24688 T24686 dobj weight,represented
R7698 T24689 T24690 amod empty,carcass
R7699 T24690 T24688 compound carcass,weight
R7700 T24691 T24688 punct (,weight
R7701 T24692 T24688 appos ECW,weight
R7702 T24693 T24686 punct ),represented
R7703 T24694 T24686 punct .,represented
R7704 T24696 T24697 nsubjpass Carcasses,labeled
R7705 T24698 T24697 auxpass were,labeled
R7706 T24699 T24697 cc and,labeled
R7707 T24700 T24697 conj secured,labeled
R7708 T24701 T24700 prep in,secured
R7709 T24702 T24703 nummod two,layers
R7710 T24703 T24701 pobj layers,in
R7711 T24704 T24703 prep of,layers
R7712 T24705 T24704 pobj cheesecloth,of
R7713 T24706 T24707 punct (,Fisher
R7714 T24707 T24705 parataxis Fisher,cheesecloth
R7715 T24708 T24707 punct ),Fisher
R7716 T24709 T24700 cc and,secured
R7717 T24710 T24700 conj frozen,secured
R7718 T24711 T24710 prep at,frozen
R7719 T24712 T24713 punct -,20
R7720 T24713 T24714 nummod 20,°C
R7721 T24714 T24711 pobj °C,at
R7722 T24715 T24710 prep until,frozen
R7723 T24716 T24715 pobj analysis,until
R7724 T24717 T24697 punct .,labeled
R7725 T24719 T24720 prep At,dried
R7726 T24721 T24722 det this,time
R7727 T24722 T24719 pobj time,At
R7728 T24723 T24720 punct ", ",dried
R7729 T24724 T24720 nsubjpass carcasses,dried
R7730 T24725 T24720 auxpass were,dried
R7731 T24726 T24720 dep freeze,dried
R7732 T24727 T24720 punct -,dried
R7733 T24728 T24720 prep for,dried
R7734 T24729 T24730 nummod seven,days
R7735 T24730 T24728 pobj days,for
R7736 T24731 T24720 cc and,dried
R7737 T24732 T24733 compound water,content
R7738 T24733 T24734 nsubjpass content,determined
R7739 T24734 T24720 conj determined,dried
R7740 T24735 T24734 auxpass was,determined
R7741 T24736 T24734 prep by,determined
R7742 T24737 T24736 pcomp subtracting,by
R7743 T24738 T24739 det the,weight
R7744 T24739 T24737 dobj weight,subtracting
R7745 T24740 T24741 npadvmod freeze,dried
R7746 T24741 T24739 amod dried,weight
R7747 T24742 T24741 punct -,dried
R7748 T24743 T24737 prep from,subtracting
R7749 T24744 T24743 pobj ECW,from
R7750 T24745 T24720 punct .,dried
R7751 T24747 T24748 nsubjpass FAT,followed
R7752 T24749 T24748 auxpass was,followed
R7753 T24750 T24748 advmod then,followed
R7754 T24751 T24748 aux extracted,followed
R7755 T24752 T24748 prep with,followed
R7756 T24753 T24752 pobj ether,with
R7757 T24754 T24748 prep for,followed
R7758 T24755 T24756 nummod 7,days
R7759 T24756 T24754 pobj days,for
R7760 T24757 T24748 punct ", ",followed
R7761 T24758 T24748 agent by,followed
R7762 T24759 T24758 pobj acetone,by
R7763 T24760 T24748 prep for,followed
R7764 T24761 T24762 det an,days
R7765 T24762 T24760 pobj days,for
R7766 T24763 T24762 amod additional,days
R7767 T24764 T24762 nummod 7,days
R7768 T24765 T24748 prep in,followed
R7769 T24766 T24767 det a,apparatus
R7770 T24767 T24765 pobj apparatus,in
R7771 T24768 T24767 compound Soxhlet,apparatus
R7772 T24769 T24748 punct .,followed
R7773 T24771 T24772 compound Carcass,ash
R7774 T24772 T24773 nsubjpass ash,determined
R7775 T24774 T24772 punct (,ash
R7776 T24775 T24772 appos ASH,ash
R7777 T24776 T24773 punct ),determined
R7778 T24777 T24773 auxpass was,determined
R7779 T24778 T24773 advcl measuring,determined
R7780 T24779 T24780 det the,remains
R7781 T24780 T24778 dobj remains,measuring
R7782 T24781 T24778 prep after,measuring
R7783 T24782 T24783 det a,incineration
R7784 T24783 T24781 pobj incineration,after
R7785 T24784 T24785 nummod 16,hour
R7786 T24785 T24783 compound hour,incineration
R7787 T24786 T24783 prep at,incineration
R7788 T24787 T24788 nummod 575,°C
R7789 T24788 T24786 pobj °C,at
R7790 T24789 T24773 punct .,determined
R7791 T24791 T24792 compound Carcass,protein
R7792 T24792 T24793 nsubjpass protein,calculated
R7793 T24794 T24792 punct (,protein
R7794 T24795 T24792 appos PROT,protein
R7795 T24796 T24793 punct ),calculated
R7796 T24797 T24793 auxpass was,calculated
R7797 T24798 T24793 prep as,calculated
R7798 T24799 T24800 det the,portion
R7799 T24800 T24798 pobj portion,as
R7800 T24801 T24800 amod remaining,portion
R7801 T24802 T24803 mark after,subtracted
R7802 T24803 T24800 advcl subtracted,portion
R7803 T24804 T24805 compound carcass,fat
R7804 T24805 T24803 nsubjpass fat,subtracted
R7805 T24806 T24805 punct (,fat
R7806 T24807 T24805 appos FAT,fat
R7807 T24808 T24805 punct ),fat
R7808 T24809 T24805 cc and,fat
R7809 T24810 T24805 conj ASH,fat
R7810 T24811 T24803 auxpass were,subtracted
R7811 T24812 T24803 prep from,subtracted
R7812 T24813 T24812 pobj ECW,from
R7813 T24814 T24793 punct .,calculated
R7814 T25106 T25107 amod Statistical,analysis
R7815 T25109 T25110 det The,procedures
R7816 T25110 T25114 nsubjpass procedures,used
R7817 T25111 T25110 nmod MEANS,procedures
R7818 T25112 T25111 cc and,MEANS
R7819 T25113 T25111 conj UNIVARIATE,MEANS
R7820 T25115 T25110 prep of,procedures
R7821 T25116 T25115 pobj SAS,of
R7822 T25117 T25114 auxpass were,used
R7823 T25118 T25119 aux to,generate
R7824 T25119 T25114 advcl generate,used
R7825 T25120 T25121 amod descriptive,statistics
R7826 T25121 T25119 dobj statistics,generate
R7827 T25122 T25119 cc and,generate
R7828 T25123 T25119 conj test,generate
R7829 T25124 T25125 compound normality,assumptions
R7830 T25125 T25123 dobj assumptions,test
R7831 T25126 T25125 prep for,assumptions
R7832 T25127 T25128 det each,trait
R7833 T25128 T25126 pobj trait,for
R7834 T25129 T25130 punct [,55
R7835 T25130 T25114 parataxis 55,used
R7836 T25131 T25130 punct ],55
R7837 T25132 T25114 punct .,used
R7838 T25134 T25135 det All,data
R7839 T25135 T25136 nsubjpass data,analyzed
R7840 T25136 T25139 ccomp analyzed,used
R7841 T25137 T25136 auxpass were,analyzed
R7842 T25138 T25136 advmod then,analyzed
R7843 T25140 T25136 advcl using,analyzed
R7844 T25141 T25142 det the,procedure
R7845 T25142 T25140 dobj procedure,using
R7846 T25143 T25142 compound GLM,procedure
R7847 T25144 T25142 prep of,procedure
R7848 T25145 T25144 pobj SAS,of
R7849 T25146 T25147 punct [,55
R7850 T25147 T25140 parataxis 55,using
R7851 T25148 T25147 punct ],55
R7852 T25149 T25140 prep with,using
R7853 T25150 T25151 det a,model
R7854 T25151 T25149 pobj model,with
R7855 T25152 T25151 amod linear,model
R7856 T25153 T25154 dep that,included
R7857 T25154 T25151 relcl included,model
R7858 T25155 T25156 det the,effects
R7859 T25156 T25154 dobj effects,included
R7860 T25157 T25156 amod fixed,effects
R7861 T25158 T25156 prep of,effects
R7862 T25159 T25158 pobj strain,of
R7863 T25160 T25159 punct ", ",strain
R7864 T25161 T25159 conj sex,strain
R7865 T25162 T25161 cc and,sex
R7866 T25163 T25164 nmod strain,interaction
R7867 T25164 T25161 conj interaction,sex
R7868 T25165 T25163 prep by,strain
R7869 T25166 T25165 pobj sex,by
R7870 T25167 T25139 punct ;,used
R7871 T25168 T25169 poss dam,weight
R7872 T25169 T25139 nsubjpass weight,used
R7873 T25170 T25168 case 's,dam
R7874 T25171 T25169 prep at,weight
R7875 T25172 T25171 pobj breeding,at
R7876 T25173 T25172 prep by,breeding
R7877 T25174 T25173 pobj strain,by
R7878 T25175 T25139 auxpass was,used
R7879 T25176 T25139 prep as,used
R7880 T25177 T25178 det a,covariate
R7881 T25178 T25176 pobj covariate,as
R7882 T25179 T25139 punct .,used
R7883 T25181 T25182 det A,model
R7884 T25182 T25185 nsubjpass model,used
R7885 T25183 T25182 amod second,model
R7886 T25184 T25182 amod linear,model
R7887 T25186 T25185 auxpass was,used
R7888 T25187 T25188 aux to,test
R7889 T25188 T25185 advcl test,used
R7890 T25189 T25188 prep for,test
R7891 T25190 T25191 nmod strain,interactions
R7892 T25191 T25189 pobj interactions,for
R7893 T25192 T25190 prep by,strain
R7894 T25193 T25194 compound hg,genotype
R7895 T25194 T25192 pobj genotype,by
R7896 T25195 T25196 punct (,+
R7897 T25196 T25191 punct +,interactions
R7898 T25197 T25196 punct +,+
R7899 T25198 T25196 punct /,+
R7900 T25199 T25196 cc or,+
R7901 T25200 T25201 compound hg,hg
R7902 T25201 T25196 conj hg,+
R7903 T25202 T25201 punct /,hg
R7904 T25203 T25196 punct ),+
R7905 T25204 T25185 punct .,used
R7906 T25206 T25207 det This,model
R7907 T25207 T25208 nsubj model,included
R7908 T25209 T25210 det the,effects
R7909 T25210 T25208 dobj effects,included
R7910 T25211 T25210 amod fixed,effects
R7911 T25212 T25210 prep of,effects
R7912 T25213 T25214 compound donor,region
R7913 T25214 T25212 pobj region,of
R7914 T25215 T25214 punct ", ",region
R7915 T25216 T25214 conj sex,region
R7916 T25217 T25216 cc and,sex
R7917 T25218 T25219 compound HG,genotype
R7918 T25219 T25216 conj genotype,sex
R7919 T25220 T25210 cc and,effects
R7920 T25221 T25222 det all,interactions
R7921 T25222 T25210 conj interactions,effects
R7922 T25223 T25222 amod possible,interactions
R7923 T25224 T25225 nummod two,way
R7924 T25225 T25222 compound way,interactions
R7925 T25226 T25224 cc and,two
R7926 T25227 T25224 conj three,two
R7927 T25228 T25225 punct -,way
R7928 T25229 T25208 punct .,included
R7929 T25231 T25232 csubj Choosing,established
R7930 T25233 T25234 det a,value
R7931 T25234 T25231 dobj value,Choosing
R7932 T25235 T25234 amod nominal,value
R7933 T25236 T25234 compound P,value
R7934 T25237 T25234 prep of,value
R7935 T25238 T25237 pobj 0.05,of
R7936 T25239 T25231 cc and,Choosing
R7937 T25240 T25231 conj applying,Choosing
R7938 T25241 T25242 det the,correction
R7939 T25242 T25240 dobj correction,applying
R7940 T25243 T25242 compound Bonferroni,correction
R7941 T25244 T25242 prep for,correction
R7942 T25245 T25246 amod multiple,comparisons
R7943 T25246 T25244 pobj comparisons,for
R7944 T25247 T25248 amod significant,differences
R7945 T25248 T25232 dobj differences,established
R7946 T25249 T25248 prep in,differences
R7947 T25250 T25251 det the,ANOVA
R7948 T25251 T25249 pobj ANOVA,in
R7949 T25252 T25251 nmod 's,ANOVA
R7950 T25253 T25232 punct .,established
R7951 T25255 T25256 det The,values
R7952 T25256 T25259 nsubjpass values,indicated
R7953 T25257 T25256 amod critical,values
R7954 T25258 T25256 compound P,values
R7955 T25260 T25256 acl used,values
R7956 T25261 T25259 auxpass are,indicated
R7957 T25262 T25259 prep in,indicated
R7958 T25263 T25264 det each,table
R7959 T25264 T25262 pobj table,in
R7960 T25265 T25259 punct .,indicated
R7961 T25441 T25440 prep of,Identification
R7962 T25442 T25443 compound candidate,genes
R7963 T25443 T25441 pobj genes,of
R7964 T25445 T25446 nsubjpass Genes,identified
R7965 T25447 T25446 auxpass were,identified
R7966 T25448 T25446 agent by,identified
R7967 T25449 T25450 amod manual,mining
R7968 T25450 T25448 pobj mining,by
R7969 T25451 T25450 compound data,mining
R7970 T25452 T25450 prep of,mining
R7971 T25453 T25454 amod primary,literature
R7972 T25454 T25452 pobj literature,of
R7973 T25455 T25454 punct ", ",literature
R7974 T25456 T25454 conj reviews,literature
R7975 T25457 T25456 cc and,reviews
R7976 T25458 T25459 compound book,chapters
R7977 T25459 T25456 conj chapters,reviews
R7978 T25460 T25446 punct .,identified
R7979 T25462 T25463 aux To,organize
R7980 T25463 T25464 advcl organize,created
R7981 T25465 T25463 cc and,organize
R7982 T25466 T25463 conj collate,organize
R7983 T25467 T25468 amod genomic,information
R7984 T25468 T25466 dobj information,collate
R7985 T25469 T25467 cc and,genomic
R7986 T25470 T25467 conj functional,genomic
R7987 T25471 T25468 prep for,information
R7988 T25472 T25473 det each,gene
R7989 T25473 T25471 pobj gene,for
R7990 T25474 T25464 nsubj we,created
R7991 T25475 T25476 det a,Annotator
R7992 T25476 T25464 dobj Annotator,created
R7993 T25477 T25478 compound custom,Gh
R7994 T25478 T25479 compound Gh,signaling
R7995 T25479 T25476 compound signaling,Annotator
R7996 T25480 T25481 compound Gene,Map
R7997 T25481 T25476 compound Map,Annotator
R7998 T25482 T25476 cc and,Annotator
R7999 T25483 T25484 nmod Pathway,Profiler
R8000 T25484 T25485 nmod Profiler,pathway
R8001 T25485 T25476 conj pathway,Annotator
R8002 T25486 T25487 punct (,GenMAPP
R8003 T25487 T25484 parataxis GenMAPP,Profiler
R8004 T25488 T25487 punct ),GenMAPP
R8005 T25489 T25490 punct [,56
R8006 T25490 T25464 parataxis 56,created
R8007 T25491 T25490 punct ],56
R8008 T25492 T25493 punct (,Figure
R8009 T25493 T25464 parataxis Figure,created
R8010 T25494 T25493 nummod 5,Figure
R8011 T25495 T25493 punct ),Figure
R8012 T25496 T25464 punct .,created
R8013 T25498 T25499 nsubj Visualization,aided
R8014 T25500 T25498 cc and,Visualization
R8015 T25501 T25498 conj color,Visualization
R8016 T25502 T25501 punct -,color
R8017 T25503 T25501 amod coding,color
R8018 T25504 T25498 prep of,Visualization
R8019 T25505 T25504 pobj genes,of
R8020 T25506 T25498 acl using,Visualization
R8021 T25507 T25506 dobj GenMAPP,using
R8022 T25508 T25509 det the,selection
R8023 T25509 T25499 dobj selection,aided
R8024 T25510 T25509 prep of,selection
R8025 T25511 T25512 compound candidate,genes
R8026 T25512 T25510 pobj genes,of
R8027 T25513 T25512 acl mapping,genes
R8028 T25514 T25513 prep within,mapping
R8029 T25515 T25516 compound QTL,regions
R8030 T25516 T25514 pobj regions,within
R8031 T25517 T25516 prep on,regions
R8032 T25518 T25519 nmod MMU,2
R8033 T25519 T25517 pobj 2,on
R8034 T25520 T25519 punct ", ",2
R8035 T25521 T25519 conj 9,2
R8036 T25522 T25521 punct ", ",9
R8037 T25523 T25521 conj 11,9
R8038 T25524 T25523 cc and,11
R8039 T25525 T25523 conj 17,11
R8040 T25526 T25499 punct .,aided
R8043 T26350 T26351 compound Candidate,gene
R8044 T26351 T26352 compound gene,sequencing
R8045 T26354 T26355 compound PCR,amplicons
R8046 T26355 T26356 nsubjpass amplicons,amplified
R8047 T26357 T26355 acl covering,amplicons
R8048 T26358 T26359 det the,sequence
R8049 T26359 T26357 dobj sequence,covering
R8050 T26360 T26359 compound coding,sequence
R8051 T26361 T26355 cc and,amplicons
R8052 T26362 T26363 amod partial,regions
R8053 T26363 T26355 conj regions,amplicons
R8054 T26364 T26363 nummod 5,regions
R8055 T26365 T26364 punct ',5
R8056 T26366 T26364 cc and,5
R8057 T26367 T26364 conj 3,5
R8058 T26368 T26367 punct ',3
R8059 T26369 T26363 amod untranslated,regions
R8060 T26370 T26363 prep for,regions
R8061 T26371 T26372 det each,gene
R8062 T26372 T26370 pobj gene,for
R8063 T26373 T26372 amod selected,gene
R8064 T26374 T26372 compound candidate,gene
R8065 T26375 T26356 auxpass were,amplified
R8066 T26376 T26356 punct ", ",amplified
R8067 T26377 T26356 conj purified,amplified
R8068 T26378 T26377 cc and,purified
R8069 T26379 T26377 conj sequenced,purified
R8070 T26380 T26379 prep from,sequenced
R8071 T26381 T26382 det the,strain
R8072 T26382 T26380 pobj strain,from
R8073 T26383 T26382 compound CAST,strain
R8074 T26384 T26379 advcl using,sequenced
R8075 T26385 T26384 dobj protocols,using
R8076 T26386 T26385 acl outlined,protocols
R8077 T26387 T26386 prep in,outlined
R8078 T26388 T26389 punct [,57
R8079 T26389 T26387 pobj 57,in
R8080 T26390 T26389 punct ],57
R8081 T26391 T26385 prep with,protocols
R8082 T26392 T26393 amod slight,modifications
R8083 T26393 T26391 pobj modifications,with
R8084 T26394 T26356 punct .,amplified
R8085 T26396 T26397 amod Total,RNA
R8086 T26397 T26398 nsubjpass RNA,isolated
R8087 T26399 T26397 prep from,RNA
R8088 T26400 T26399 pobj brain,from
R8089 T26401 T26400 punct ", ",brain
R8090 T26402 T26400 conj liver,brain
R8091 T26403 T26402 punct ", ",liver
R8092 T26404 T26402 conj spleen,liver
R8093 T26405 T26404 punct ", ",spleen
R8094 T26406 T26404 conj lung,spleen
R8095 T26407 T26406 cc and,lung
R8096 T26408 T26406 conj testis,lung
R8097 T26409 T26398 auxpass was,isolated
R8098 T26410 T26398 prep from,isolated
R8099 T26411 T26412 det an,mouse
R8100 T26412 T26410 pobj mouse,from
R8101 T26413 T26412 amod adult,mouse
R8102 T26414 T26412 nmod CAST,mouse
R8103 T26415 T26412 amod male,mouse
R8104 T26416 T26398 advcl using,isolated
R8105 T26417 T26416 dobj Trizol,using
R8106 T26418 T26419 punct (,Ambion
R8107 T26419 T26417 parataxis Ambion,Trizol
R8108 T26420 T26419 punct ),Ambion
R8109 T26421 T26398 punct .,isolated
R8110 T26423 T26424 nsubjpass cDNA,produced
R8111 T26425 T26424 auxpass was,produced
R8112 T26426 T26424 prep from,produced
R8113 T26427 T26428 amod total,RNA
R8114 T26428 T26426 pobj RNA,from
R8115 T26429 T26424 advcl using,produced
R8116 T26430 T26431 amod standard,procedures
R8117 T26431 T26429 dobj procedures,using
R8118 T26432 T26424 punct .,produced
R8119 T26434 T26435 compound PCR,sets
R8120 T26435 T26437 nsubjpass sets,designed
R8121 T26436 T26435 compound primer,sets
R8122 T26438 T26435 prep for,sets
R8123 T26439 T26440 det each,gene
R8124 T26440 T26438 pobj gene,for
R8125 T26441 T26437 auxpass were,designed
R8126 T26442 T26437 advcl using,designed
R8127 T26443 T26442 dobj Primer3,using
R8128 T26444 T26445 punct [,58
R8129 T26445 T26437 parataxis 58,designed
R8130 T26446 T26445 punct ],58
R8131 T26447 T26448 punct (,File
R8132 T26448 T26437 parataxis File,designed
R8133 T26449 T26448 amod Additional,File
R8134 T26450 T26448 nummod 5,File
R8135 T26451 T26448 punct ),File
R8136 T26452 T26437 punct .,designed
R8137 T26454 T26455 det The,amplification
R8138 T26455 T26457 nsubjpass amplification,performed
R8139 T26456 T26455 amod initial,amplification
R8140 T26458 T26457 auxpass was,performed
R8141 T26459 T26457 prep in,performed
R8142 T26460 T26461 nummod 10,μl
R8143 T26461 T26462 compound μl,reactions
R8144 T26462 T26459 pobj reactions,in
R8145 T26463 T26462 compound PCR,reactions
R8146 T26464 T26457 advcl using,performed
R8147 T26465 T26466 det the,system
R8148 T26466 T26464 dobj system,using
R8149 T26467 T26466 compound Invitrogen,system
R8150 T26468 T26466 compound Platinum,system
R8151 T26469 T26470 compound TaqPCRx,amplification
R8152 T26470 T26466 compound amplification,system
R8153 T26471 T26472 punct (,Invitrogen
R8154 T26472 T26466 parataxis Invitrogen,system
R8155 T26473 T26472 punct ),Invitrogen
R8156 T26474 T26457 punct .,performed
R8157 T26476 T26477 det The,reactions
R8158 T26477 T26478 nsubj reactions,contained
R8159 T26479 T26480 compound 1X,buffer
R8160 T26480 T26478 dobj buffer,contained
R8161 T26481 T26480 compound PCR,buffer
R8162 T26482 T26480 punct ", ",buffer
R8163 T26483 T26484 compound 1X,solution
R8164 T26484 T26480 conj solution,buffer
R8165 T26485 T26484 compound Enhancer,solution
R8166 T26486 T26484 punct ", ",solution
R8167 T26487 T26488 nummod 1.5,mM
R8168 T26488 T26489 compound mM,MgSO4
R8169 T26489 T26484 conj MgSO4,solution
R8170 T26490 T26489 punct ", ",MgSO4
R8171 T26491 T26492 nummod 0.17,mM
R8172 T26492 T26493 compound mM,dNTPs
R8173 T26493 T26489 conj dNTPs,MgSO4
R8174 T26494 T26493 punct ", ",dNTPs
R8175 T26495 T26496 nummod 1,μM
R8176 T26496 T26497 nmod μM,primer
R8177 T26497 T26493 conj primer,dNTPs
R8178 T26498 T26497 det each,primer
R8179 T26499 T26497 punct ", ",primer
R8180 T26500 T26501 nummod 0.1,unit
R8181 T26501 T26497 conj unit,primer
R8182 T26502 T26501 prep of,unit
R8183 T26503 T26504 compound Platinum,Taq
R8184 T26504 T26502 pobj Taq,of
R8185 T26505 T26506 punct (,Invitrogen
R8186 T26506 T26504 parataxis Invitrogen,Taq
R8187 T26507 T26506 punct ),Invitrogen
R8188 T26508 T26501 cc and,unit
R8189 T26509 T26510 advmod approximately,25
R8190 T26510 T26511 nummod 25,ng
R8191 T26511 T26501 conj ng,unit
R8192 T26512 T26511 prep of,ng
R8193 T26513 T26512 pobj cDNA,of
R8194 T26514 T26478 punct .,contained
R8195 T26516 T26517 nsubjpass Reactions,incubated
R8196 T26518 T26517 auxpass were,incubated
R8197 T26519 T26517 prep for,incubated
R8198 T26520 T26521 nummod 5,min
R8199 T26521 T26519 pobj min,for
R8200 T26522 T26517 prep at,incubated
R8201 T26523 T26524 nummod 95,°C
R8202 T26524 T26522 pobj °C,at
R8203 T26525 T26517 punct ", ",incubated
R8204 T26526 T26527 advmod then,cycled
R8205 T26527 T26517 dep cycled,incubated
R8206 T26528 T26527 prep for,cycled
R8207 T26529 T26530 nummod 45,s
R8208 T26530 T26528 pobj s,for
R8209 T26531 T26530 prep at,s
R8210 T26532 T26533 nummod 95,°C
R8211 T26533 T26531 pobj °C,at
R8212 T26534 T26530 punct ", ",s
R8213 T26535 T26536 nummod 45,s
R8214 T26536 T26530 appos s,s
R8215 T26537 T26536 prep at,s
R8216 T26538 T26539 nummod 55,°C
R8217 T26539 T26537 pobj °C,at
R8218 T26540 T26530 punct ", ",s
R8219 T26541 T26542 nummod 1,min
R8220 T26542 T26530 appos min,s
R8221 T26543 T26542 prep at,min
R8222 T26544 T26545 nummod 72,°C
R8223 T26545 T26543 pobj °C,at
R8224 T26546 T26542 prep for,min
R8225 T26547 T26548 nummod 35,cycles
R8226 T26548 T26546 pobj cycles,for
R8227 T26549 T26527 prep with,cycled
R8228 T26550 T26551 det a,extension
R8229 T26551 T26549 pobj extension,with
R8230 T26552 T26551 amod final,extension
R8231 T26553 T26554 nummod 72,°C
R8232 T26554 T26551 compound °C,extension
R8233 T26555 T26551 prep for,extension
R8234 T26556 T26557 nummod 10,min
R8235 T26557 T26555 pobj min,for
R8236 T26558 T26551 prep on,extension
R8237 T26559 T26560 det a,PTC
R8238 T26560 T26558 pobj PTC,on
R8239 T26561 T26562 compound MJ,Research
R8240 T26562 T26560 compound Research,PTC
R8241 T26563 T26560 punct -,PTC
R8242 T26564 T26560 nummod 200,PTC
R8243 T26565 T26517 punct .,incubated
R8244 T26567 T26568 det The,products
R8245 T26568 T26569 nsubjpass products,visualized
R8246 T26570 T26569 auxpass were,visualized
R8247 T26571 T26569 prep on,visualized
R8248 T26572 T26573 nummod 1.5,%
R8249 T26573 T26574 compound %,agarose
R8250 T26574 T26575 compound agarose,gels
R8251 T26575 T26571 pobj gels,on
R8252 T26576 T26575 acl containing,gels
R8253 T26577 T26578 nummod 0.06,μg
R8254 T26578 T26579 nmod μg,EtBr
R8255 T26579 T26576 dobj EtBr,containing
R8256 T26580 T26581 punct /,ml
R8257 T26581 T26578 prep ml,μg
R8258 T26582 T26569 punct .,visualized
R8259 T26584 T26585 nsubjpass Bands,excised
R8260 T26586 T26584 prep of,Bands
R8261 T26587 T26588 det the,size
R8262 T26588 T26586 pobj size,of
R8263 T26589 T26588 amod correct,size
R8264 T26590 T26585 auxpass were,excised
R8265 T26591 T26585 cc and,excised
R8266 T26592 T26585 conj incubated,excised
R8267 T26593 T26592 prep in,incubated
R8268 T26594 T26595 nummod 100,μl
R8269 T26595 T26593 pobj μl,in
R8270 T26596 T26595 prep of,μl
R8271 T26597 T26598 amod sterile,H2O
R8272 T26598 T26596 pobj H2O,of
R8273 T26599 T26598 prep at,H2O
R8274 T26600 T26601 nummod 80,°C
R8275 T26601 T26599 pobj °C,at
R8276 T26602 T26592 prep for,incubated
R8277 T26603 T26604 nummod 10,min
R8278 T26604 T26602 pobj min,for
R8279 T26605 T26606 aux to,elute
R8280 T26606 T26592 advcl elute,incubated
R8281 T26607 T26606 dobj DNA,elute
R8282 T26608 T26585 punct .,excised
R8283 T26610 T26611 compound Reamplification,reactions
R8284 T26611 T26612 nsubj reactions,consisted
R8285 T26613 T26612 prep of,consisted
R8286 T26614 T26615 compound 1X,buffer
R8287 T26615 T26613 pobj buffer,of
R8288 T26616 T26615 compound PCR,buffer
R8289 T26617 T26615 punct ", ",buffer
R8290 T26618 T26619 nummod 1.5,mM
R8291 T26619 T26620 compound mM,MgCl2
R8292 T26620 T26615 conj MgCl2,buffer
R8293 T26621 T26620 punct ", ",MgCl2
R8294 T26622 T26623 nummod 0.17,mM
R8295 T26623 T26624 compound mM,dNTPs
R8296 T26624 T26620 conj dNTPs,MgCl2
R8297 T26625 T26624 punct ", ",dNTPs
R8298 T26626 T26627 nummod 1,μM
R8299 T26627 T26628 nmod μM,primer
R8300 T26628 T26624 conj primer,dNTPs
R8301 T26629 T26628 det each,primer
R8302 T26630 T26628 punct ", ",primer
R8303 T26631 T26632 nummod 0.1,unit
R8304 T26632 T26628 conj unit,primer
R8305 T26633 T26632 prep of,unit
R8306 T26634 T26633 pobj Taq,of
R8307 T26635 T26636 punct (,Promega
R8308 T26636 T26634 parataxis Promega,Taq
R8309 T26637 T26636 punct ),Promega
R8310 T26638 T26632 cc and,unit
R8311 T26639 T26640 nummod 10,μl
R8312 T26640 T26632 conj μl,unit
R8313 T26641 T26640 prep of,μl
R8314 T26642 T26641 pobj eluate,of
R8315 T26643 T26615 prep in,buffer
R8316 T26644 T26645 det a,volume
R8317 T26645 T26643 pobj volume,in
R8318 T26646 T26645 amod total,volume
R8319 T26647 T26645 prep of,volume
R8320 T26648 T26649 nummod 50,μl
R8321 T26649 T26647 pobj μl,of
R8322 T26650 T26612 punct .,consisted
R8323 T26652 T26653 det The,parameters
R8324 T26653 T26656 nsubjpass parameters,used
R8325 T26654 T26653 amod same,parameters
R8326 T26655 T26653 compound cycling,parameters
R8327 T26657 T26656 auxpass were,used
R8328 T26658 T26656 prep for,used
R8329 T26659 T26660 compound reamplification,reactions
R8330 T26660 T26658 pobj reactions,for
R8331 T26661 T26656 punct .,used
R8332 T26663 T26664 nsubjpass Products,visualized
R8333 T26665 T26664 auxpass were,visualized
R8334 T26666 T26664 prep on,visualized
R8335 T26667 T26668 nummod 1,%
R8336 T26668 T26669 compound %,gels
R8337 T26669 T26666 pobj gels,on
R8338 T26670 T26669 compound 0.5X,gels
R8339 T26671 T26669 compound TBE,gels
R8340 T26672 T26669 compound agarose,gels
R8341 T26673 T26669 acl containing,gels
R8342 T26674 T26675 nummod 0.06,μg
R8343 T26675 T26676 nmod μg,EtBr
R8344 T26676 T26673 dobj EtBr,containing
R8345 T26677 T26678 punct /,ml
R8346 T26678 T26675 prep ml,μg
R8347 T26679 T26664 punct ", ",visualized
R8348 T26680 T26664 conj excised,visualized
R8349 T26681 T26680 cc and,excised
R8350 T26682 T26680 conj purfied,excised
R8351 T26683 T26682 advcl using,purfied
R8352 T26684 T26685 compound PCR,purification
R8353 T26685 T26686 compound purification,columns
R8354 T26686 T26683 dobj columns,using
R8355 T26687 T26688 punct (,Promega
R8356 T26688 T26686 parataxis Promega,columns
R8357 T26689 T26688 punct ),Promega
R8358 T26690 T26664 punct .,visualized
R8359 T26692 T26693 aux To,quantity
R8360 T26693 T26694 advcl quantity,run
R8361 T26695 T26696 det each,fragment
R8362 T26696 T26693 dobj fragment,quantity
R8363 T26697 T26698 nummod 1,μl
R8364 T26698 T26694 nsubjpass μl,run
R8365 T26699 T26698 prep of,μl
R8366 T26700 T26701 det each,product
R8367 T26701 T26699 pobj product,of
R8368 T26702 T26701 amod purified,product
R8369 T26703 T26694 auxpass was,run
R8370 T26704 T26694 prep on,run
R8371 T26705 T26706 det a,gel
R8372 T26706 T26704 pobj gel,on
R8373 T26707 T26708 nummod 1.0,%
R8374 T26708 T26706 compound %,gel
R8375 T26709 T26706 compound agarose,gel
R8376 T26710 T26706 acl containing,gel
R8377 T26711 T26712 nummod 0.06,μg
R8378 T26712 T26713 nmod μg,EtBr
R8379 T26713 T26710 dobj EtBr,containing
R8380 T26714 T26715 punct /,ml
R8381 T26715 T26712 prep ml,μg
R8382 T26716 T26706 punct ", ",gel
R8383 T26717 T26706 prep along,gel
R8384 T26718 T26717 prep with,along
R8385 T26719 T26720 det a,ladder
R8386 T26720 T26718 pobj ladder,with
R8387 T26721 T26720 compound DNA,ladder
R8388 T26722 T26720 compound mass,ladder
R8389 T26723 T26724 punct (,Invitrogen
R8390 T26724 T26720 parataxis Invitrogen,ladder
R8391 T26725 T26724 punct ),Invitrogen
R8392 T26726 T26694 punct .,run
R8393 T26728 T26729 prep In,added
R8394 T26730 T26731 det a,plate
R8395 T26731 T26728 pobj plate,In
R8396 T26732 T26733 nummod 96,well
R8397 T26733 T26731 compound well,plate
R8398 T26734 T26729 punct ", ",added
R8399 T26735 T26736 nummod 15,ng
R8400 T26736 T26729 nsubjpass ng,added
R8401 T26737 T26736 prep of,ng
R8402 T26738 T26739 det each,product
R8403 T26739 T26737 pobj product,of
R8404 T26740 T26739 compound PCR,product
R8405 T26741 T26729 auxpass was,added
R8406 T26742 T26729 prep to,added
R8407 T26743 T26744 nummod 5,pM
R8408 T26744 T26742 pobj pM,to
R8409 T26745 T26744 prep of,pM
R8410 T26746 T26745 pobj primer,of
R8411 T26747 T26729 prep for,added
R8412 T26748 T26747 pobj sequencing,for
R8413 T26749 T26729 punct .,added
R8414 T26751 T26752 det The,College
R8415 T26752 T26753 nmod College,Facility
R8416 T26753 T26763 nsubj Facility,performed
R8417 T26754 T26752 prep of,College
R8418 T26755 T26756 nmod Agriculture,Sciences
R8419 T26756 T26754 pobj Sciences,of
R8420 T26757 T26755 cc and,Agriculture
R8421 T26758 T26755 conj Environmental,Agriculture
R8422 T26759 T26752 punct (,College
R8423 T26760 T26752 appos CAES,College
R8424 T26761 T26753 punct ),Facility
R8425 T26762 T26753 compound Genomics,Facility
R8426 T26764 T26753 prep at,Facility
R8427 T26765 T26766 det the,University
R8428 T26766 T26764 pobj University,at
R8429 T26767 T26766 prep of,University
R8430 T26768 T26767 pobj California,of
R8431 T26769 T26766 punct ", ",University
R8432 T26770 T26766 npadvmod Davis,University
R8433 T26771 T26763 punct ", ",performed
R8434 T26772 T26773 amod bidirectional,sequencing
R8435 T26773 T26763 dobj sequencing,performed
R8436 T26774 T26773 prep of,sequencing
R8437 T26775 T26776 det each,amplicon
R8438 T26776 T26774 pobj amplicon,of
R8439 T26777 T26763 punct .,performed
R8440 T26935 T26936 compound Sequence,analysis
R8441 T26938 T26939 compound B6,sequences
R8442 T26939 T26941 nsubjpass sequences,downloaded
R8443 T26940 T26939 compound mRNA,sequences
R8444 T26942 T26939 prep for,sequences
R8445 T26943 T26944 det each,gene
R8446 T26944 T26942 pobj gene,for
R8447 T26945 T26941 auxpass were,downloaded
R8448 T26946 T26941 prep from,downloaded
R8449 T26947 T26948 det the,assembly
R8450 T26948 T26946 pobj assembly,from
R8451 T26949 T26948 nmod May,assembly
R8452 T26950 T26949 nummod 2004,May
R8453 T26951 T26952 punct (,mm5
R8454 T26952 T26948 parataxis mm5,assembly
R8455 T26953 T26952 punct ),mm5
R8456 T26954 T26948 nmod UCSC,assembly
R8457 T26955 T26956 punct (,Build
R8458 T26956 T26948 parataxis Build,assembly
R8459 T26957 T26956 compound NCBI,Build
R8460 T26958 T26956 nummod 33,Build
R8461 T26959 T26956 punct ),Build
R8462 T26960 T26948 compound genome,assembly
R8463 T26961 T26962 punct [,28
R8464 T26962 T26941 parataxis 28,downloaded
R8465 T26963 T26962 punct ],28
R8466 T26964 T26941 punct .,downloaded
R8467 T26966 T26967 det These,sequences
R8468 T26967 T26968 nsubjpass sequences,imported
R8469 T26969 T26968 auxpass were,imported
R8470 T26970 T26968 prep along,imported
R8471 T26971 T26970 prep with,along
R8472 T26972 T26973 det all,traces
R8473 T26973 T26971 pobj traces,with
R8474 T26974 T26975 compound CAST,sequence
R8475 T26975 T26973 compound sequence,traces
R8476 T26976 T26968 prep into,imported
R8477 T26977 T26978 det the,program
R8478 T26978 T26976 pobj program,into
R8479 T26979 T26978 compound SeqMan,program
R8480 T26980 T26981 compound sequence,assembly
R8481 T26981 T26978 compound assembly,program
R8482 T26982 T26983 punct (,DNASTAR
R8483 T26983 T26978 parataxis DNASTAR,program
R8484 T26984 T26983 punct ),DNASTAR
R8485 T26985 T26968 cc and,imported
R8486 T26986 T26987 advmod manually,curated
R8487 T26987 T26968 conj curated,imported
R8488 T26988 T26987 prep for,curated
R8489 T26989 T26988 pobj quality,for
R8490 T26990 T26968 punct .,imported
R8491 T26992 T26993 amod Poor,quality
R8492 T26993 T26994 compound quality,reads
R8493 T26994 T26995 nsubjpass reads,resequenced
R8494 T26996 T26995 auxpass were,resequenced
R8495 T26997 T26995 punct .,resequenced
R8496 T26999 T27000 amod Individual,contigs
R8497 T27000 T27001 nsubjpass contigs,created
R8498 T27002 T27001 auxpass were,created
R8499 T27003 T27001 prep for,created
R8500 T27004 T27005 det each,gene
R8501 T27005 T27003 pobj gene,for
R8502 T27006 T27001 cc and,created
R8503 T27007 T27008 nsubjpass polymorphisms,detected
R8504 T27008 T27001 conj detected,created
R8505 T27009 T27008 auxpass were,detected
R8506 T27010 T27008 cc and,detected
R8507 T27011 T27008 conj curated,detected
R8508 T27012 T27011 prep by,curated
R8509 T27013 T27014 amod manual,inspection
R8510 T27014 T27012 pobj inspection,by
R8511 T27015 T27008 punct .,detected